LOCUS       DAZFYC010000001       117552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFYC010000001 DAZFYC010000000
VERSION     DAZFYC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 117552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 117552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.001"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHKW5_000001"
     CDS             complement(<1..209)
                     /locus_tag="ACHKW5_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7509464.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKW5_000002"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKW5_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO7509465.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKW5_000003"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKW5_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO7509466.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKW5_000004"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKW5_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO7509467.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKW5_000005"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKW5_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO7509468.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLIGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKW5_000006"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKW5_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO7509469.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKW5_000007"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKW5_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO7509470.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTYDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKW5_000008"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKW5_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7509471.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKW5_000009"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKW5_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7509472.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKW5_000010"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKW5_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO7509473.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKW5_000011"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKW5_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7509474.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKW5_000012"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKW5_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO7509475.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKW5_000013"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKW5_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO7509476.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKW5_000014"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKW5_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO7509477.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLVG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKW5_000015"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKW5_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7509478.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKW5_000016"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKW5_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7509479.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKSFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKW5_000017"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKW5_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO7509480.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKW5_000018"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKW5_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO7509481.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKW5_000019"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKW5_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO7509482.1"
                     /translation="MQARVKWVEGLIFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKW5_000020"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKW5_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO7509483.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKW5_000021"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKW5_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO7509484.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKW5_000022"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKW5_000022"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO7509485.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKW5_000023"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKW5_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO7509486.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKW5_000024"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKW5_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO7509487.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKW5_000025"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKW5_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO7509488.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKW5_000026"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKW5_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO7509489.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKW5_000027"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKW5_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO7509490.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKW5_000028"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKW5_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO7509491.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTEDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKW5_000029"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKW5_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO7509492.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKW5_000030"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="ACHKW5_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO7509493.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKW5_000031"
     CDS             26708..28081
                     /gene="cysG"
                     /locus_tag="ACHKW5_000031"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO7509494.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /gene="yhfL"
                     /locus_tag="ACHKW5_000032"
     CDS             28328..28495
                     /gene="yhfL"
                     /locus_tag="ACHKW5_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO7509495.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30127
                     /gene="frlA"
                     /locus_tag="ACHKW5_000033"
     CDS             28790..30127
                     /gene="frlA"
                     /locus_tag="ACHKW5_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO7509496.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGAQGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30148..31170
                     /gene="frlB"
                     /locus_tag="ACHKW5_000034"
     CDS             30148..31170
                     /gene="frlB"
                     /locus_tag="ACHKW5_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO7509497.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQHGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31220..32050
                     /gene="frlC"
                     /locus_tag="ACHKW5_000035"
     CDS             31220..32050
                     /gene="frlC"
                     /locus_tag="ACHKW5_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO7509498.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32047..32832
                     /gene="frlD"
                     /locus_tag="ACHKW5_000036"
     CDS             32047..32832
                     /gene="frlD"
                     /locus_tag="ACHKW5_000036"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO7509499.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32932..33663
                     /gene="frlR"
                     /locus_tag="ACHKW5_000037"
                     /gene_synonym="yhfR"
     CDS             32932..33663
                     /gene="frlR"
                     /locus_tag="ACHKW5_000037"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO7509500.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33815..34900)
                     /gene="yhfS"
                     /locus_tag="ACHKW5_000038"
     CDS             complement(33815..34900)
                     /gene="yhfS"
                     /locus_tag="ACHKW5_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7509501.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANISTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34912..36216)
                     /gene="yhfT"
                     /locus_tag="ACHKW5_000039"
     CDS             complement(34912..36216)
                     /gene="yhfT"
                     /locus_tag="ACHKW5_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO7509502.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36228..36581)
                     /gene="yhfU"
                     /locus_tag="ACHKW5_000040"
     CDS             complement(36228..36581)
                     /gene="yhfU"
                     /locus_tag="ACHKW5_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO7509503.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36592..37470)
                     /gene="php"
                     /locus_tag="ACHKW5_000041"
     CDS             complement(36592..37470)
                     /gene="php"
                     /locus_tag="ACHKW5_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO7509504.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37467..38693)
                     /gene="yhfW"
                     /locus_tag="ACHKW5_000042"
     CDS             complement(37467..38693)
                     /gene="yhfW"
                     /locus_tag="ACHKW5_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7509505.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38693..39856)
                     /gene="yhfX"
                     /locus_tag="ACHKW5_000043"
     CDS             complement(38693..39856)
                     /gene="yhfX"
                     /locus_tag="ACHKW5_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO7509506.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIVIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(39940..40302)
                     /gene="yhfY"
                     /locus_tag="ACHKW5_000044"
     CDS             complement(39940..40302)
                     /gene="yhfY"
                     /locus_tag="ACHKW5_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO7509507.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40319..41224)
                     /gene="yhfZ"
                     /locus_tag="ACHKW5_000045"
     CDS             complement(40319..41224)
                     /gene="yhfZ"
                     /locus_tag="ACHKW5_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO7509508.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41493..42497)
                     /gene="trpS"
                     /locus_tag="ACHKW5_000046"
     CDS             complement(41493..42497)
                     /gene="trpS"
                     /locus_tag="ACHKW5_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO7509509.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42490..43248)
                     /gene="gph"
                     /locus_tag="ACHKW5_000047"
     CDS             complement(42490..43248)
                     /gene="gph"
                     /locus_tag="ACHKW5_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO7509510.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43241..43918)
                     /gene="rpe"
                     /locus_tag="ACHKW5_000048"
     CDS             complement(43241..43918)
                     /gene="rpe"
                     /locus_tag="ACHKW5_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO7509511.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43936..44772)
                     /gene="dam"
                     /locus_tag="ACHKW5_000049"
     CDS             complement(43936..44772)
                     /gene="dam"
                     /locus_tag="ACHKW5_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7509512.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44879..46165)
                     /gene="damX"
                     /locus_tag="ACHKW5_000050"
     CDS             complement(44879..46165)
                     /gene="damX"
                     /locus_tag="ACHKW5_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO7509513.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPATKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYGNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46257..47345)
                     /gene="aroB"
                     /locus_tag="ACHKW5_000051"
     CDS             complement(46257..47345)
                     /gene="aroB"
                     /locus_tag="ACHKW5_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO7509514.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47402..47923)
                     /gene="aroK"
                     /locus_tag="ACHKW5_000052"
     CDS             complement(47402..47923)
                     /gene="aroK"
                     /locus_tag="ACHKW5_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO7509515.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48324..49562)
                     /gene="hofQ"
                     /locus_tag="ACHKW5_000053"
     CDS             complement(48324..49562)
                     /gene="hofQ"
                     /locus_tag="ACHKW5_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO7509516.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49474..49878)
                     /gene="hofP"
                     /locus_tag="ACHKW5_000054"
     CDS             complement(49474..49878)
                     /gene="hofP"
                     /locus_tag="ACHKW5_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO7509517.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49868..50308)
                     /gene="hofO"
                     /locus_tag="ACHKW5_000055"
     CDS             complement(49868..50308)
                     /gene="hofO"
                     /locus_tag="ACHKW5_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO7509518.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50292..50831)
                     /gene="hofN"
                     /locus_tag="ACHKW5_000056"
     CDS             complement(50292..50831)
                     /gene="hofN"
                     /locus_tag="ACHKW5_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO7509519.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50831..51610)
                     /gene="hofM"
                     /locus_tag="ACHKW5_000057"
     CDS             complement(50831..51610)
                     /gene="hofM"
                     /locus_tag="ACHKW5_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO7509520.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            51730..54282
                     /gene="mrcA"
                     /locus_tag="ACHKW5_000058"
     CDS             51730..54282
                     /gene="mrcA"
                     /locus_tag="ACHKW5_000058"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO7509521.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54447..55007)
                     /gene="nudE"
                     /locus_tag="ACHKW5_000059"
     CDS             complement(54447..55007)
                     /gene="nudE"
                     /locus_tag="ACHKW5_000059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO7509522.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55327..57462
                     /gene="igaA"
                     /locus_tag="ACHKW5_000060"
     CDS             55327..57462
                     /gene="igaA"
                     /locus_tag="ACHKW5_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO7509523.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57527..58195
                     /gene="yrfG"
                     /locus_tag="ACHKW5_000061"
     CDS             57527..58195
                     /gene="yrfG"
                     /locus_tag="ACHKW5_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO7509524.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58206..58607
                     /gene="hslR"
                     /locus_tag="ACHKW5_000062"
     CDS             58206..58607
                     /gene="hslR"
                     /locus_tag="ACHKW5_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO7509525.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58632..59510
                     /gene="hslO"
                     /locus_tag="ACHKW5_000063"
     CDS             58632..59510
                     /gene="hslO"
                     /locus_tag="ACHKW5_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO7509526.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59645..61369)
                     /gene="yhgE"
                     /locus_tag="ACHKW5_000064"
     CDS             complement(59645..61369)
                     /gene="yhgE"
                     /locus_tag="ACHKW5_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO7509527.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61747..63369
                     /gene="pckA"
                     /locus_tag="ACHKW5_000065"
     CDS             61747..63369
                     /gene="pckA"
                     /locus_tag="ACHKW5_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO7509528.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63445..64797)
                     /gene="envZ"
                     /locus_tag="ACHKW5_000066"
     CDS             complement(63445..64797)
                     /gene="envZ"
                     /locus_tag="ACHKW5_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO7509529.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64794..65513)
                     /gene="ompR"
                     /locus_tag="ACHKW5_000067"
     CDS             complement(64794..65513)
                     /gene="ompR"
                     /locus_tag="ACHKW5_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO7509530.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65741..66217
                     /gene="greB"
                     /locus_tag="ACHKW5_000068"
     CDS             65741..66217
                     /gene="greB"
                     /locus_tag="ACHKW5_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO7509531.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66314..68635
                     /gene="yhgF"
                     /locus_tag="ACHKW5_000069"
     CDS             66314..68635
                     /gene="yhgF"
                     /locus_tag="ACHKW5_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO7509532.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69073..69300
                     /gene="feoA"
                     /locus_tag="ACHKW5_000070"
     CDS             69073..69300
                     /gene="feoA"
                     /locus_tag="ACHKW5_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO7509533.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69317..71638
                     /gene="feoB"
                     /locus_tag="ACHKW5_000071"
     CDS             69317..71638
                     /gene="feoB"
                     /locus_tag="ACHKW5_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO7509534.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEMQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71638..71874
                     /gene="feoC"
                     /locus_tag="ACHKW5_000072"
     CDS             71638..71874
                     /gene="feoC"
                     /locus_tag="ACHKW5_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO7509535.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72077..72955
                     /gene="rpnA"
                     /locus_tag="ACHKW5_000073"
     CDS             72077..72955
                     /gene="rpnA"
                     /locus_tag="ACHKW5_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO7509536.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGQVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(72984..73754)
                     /gene="bioH"
                     /locus_tag="ACHKW5_000074"
     CDS             complement(72984..73754)
                     /gene="bioH"
                     /locus_tag="ACHKW5_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO7509537.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73792..74475
                     /gene="gntX"
                     /locus_tag="ACHKW5_000075"
     CDS             73792..74475
                     /gene="gntX"
                     /locus_tag="ACHKW5_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO7509538.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSKAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74534..75109
                     /gene="nfuA"
                     /locus_tag="ACHKW5_000076"
     CDS             74534..75109
                     /gene="nfuA"
                     /locus_tag="ACHKW5_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO7509539.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75470..76786
                     /gene="gntT"
                     /locus_tag="ACHKW5_000077"
     CDS             75470..76786
                     /gene="gntT"
                     /locus_tag="ACHKW5_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7509540.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(76831..78915)
                     /gene="malQ"
                     /locus_tag="ACHKW5_000078"
     CDS             complement(76831..78915)
                     /gene="malQ"
                     /locus_tag="ACHKW5_000078"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO7509541.1"
                     /translation="MESKRLDNAALAAGISPNYINAYGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDHE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(78925..81318)
                     /gene="malP"
                     /locus_tag="ACHKW5_000079"
     CDS             complement(78925..81318)
                     /gene="malP"
                     /locus_tag="ACHKW5_000079"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO7509542.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            81930..84635
                     /gene="malT"
                     /locus_tag="ACHKW5_000080"
     CDS             81930..84635
                     /gene="malT"
                     /locus_tag="ACHKW5_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO7509543.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(84678..85694)
                     /gene="rtcA"
                     /locus_tag="ACHKW5_000081"
     CDS             complement(84678..85694)
                     /gene="rtcA"
                     /locus_tag="ACHKW5_000081"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO7509544.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGIVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(85698..86924)
                     /gene="rtcB"
                     /locus_tag="ACHKW5_000082"
     CDS             complement(85698..86924)
                     /gene="rtcB"
                     /locus_tag="ACHKW5_000082"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO7509545.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            87113..88711
                     /gene="rtcR"
                     /locus_tag="ACHKW5_000083"
     CDS             87113..88711
                     /gene="rtcR"
                     /locus_tag="ACHKW5_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO7509546.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSVFQLISGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(88693..89451)
                     /locus_tag="ACHKW5_000084"
     CDS             complement(88693..89451)
                     /locus_tag="ACHKW5_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO7509547.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(89468..90298)
                     /gene="glpG"
                     /locus_tag="ACHKW5_000085"
     CDS             complement(89468..90298)
                     /gene="glpG"
                     /locus_tag="ACHKW5_000085"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO7509548.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(90343..90669)
                     /gene="glpE"
                     /locus_tag="ACHKW5_000086"
     CDS             complement(90343..90669)
                     /gene="glpE"
                     /locus_tag="ACHKW5_000086"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO7509549.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            90859..92364
                     /gene="glpD"
                     /locus_tag="ACHKW5_000087"
     CDS             90859..92364
                     /gene="glpD"
                     /locus_tag="ACHKW5_000087"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO7509550.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(92416..93021)
                     /locus_tag="ACHKW5_000088"
     CDS             complement(92416..93021)
                     /locus_tag="ACHKW5_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509551.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(93024..93791)
                     /locus_tag="ACHKW5_000089"
     CDS             complement(93024..93791)
                     /locus_tag="ACHKW5_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509552.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDETVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(93794..94504)
                     /locus_tag="ACHKW5_000090"
     CDS             complement(93794..94504)
                     /locus_tag="ACHKW5_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509553.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(94520..96028)
                     /locus_tag="ACHKW5_000091"
     CDS             complement(94520..96028)
                     /locus_tag="ACHKW5_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7509554.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(96153..98600)
                     /gene="glgP"
                     /locus_tag="ACHKW5_000092"
     CDS             complement(96153..98600)
                     /gene="glgP"
                     /locus_tag="ACHKW5_000092"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO7509555.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(98619..100052)
                     /gene="glgA"
                     /locus_tag="ACHKW5_000093"
     CDS             complement(98619..100052)
                     /gene="glgA"
                     /locus_tag="ACHKW5_000093"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO7509556.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(100052..101347)
                     /gene="glgC"
                     /locus_tag="ACHKW5_000094"
     CDS             complement(100052..101347)
                     /gene="glgC"
                     /locus_tag="ACHKW5_000094"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO7509557.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(101365..103338)
                     /gene="glgX"
                     /locus_tag="ACHKW5_000095"
     CDS             complement(101365..103338)
                     /gene="glgX"
                     /locus_tag="ACHKW5_000095"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO7509558.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(103335..105521)
                     /gene="glgB"
                     /locus_tag="ACHKW5_000096"
     CDS             complement(103335..105521)
                     /gene="glgB"
                     /locus_tag="ACHKW5_000096"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO7509559.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(105794..106897)
                     /gene="asd"
                     /locus_tag="ACHKW5_000097"
     CDS             complement(105794..106897)
                     /gene="asd"
                     /locus_tag="ACHKW5_000097"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7509560.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            107090..107683
                     /gene="yhgN"
                     /locus_tag="ACHKW5_000098"
     CDS             107090..107683
                     /gene="yhgN"
                     /locus_tag="ACHKW5_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO7509561.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(107729..109024)
                     /locus_tag="ACHKW5_000099"
     CDS             complement(107729..109024)
                     /locus_tag="ACHKW5_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO7509562.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHENDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(108931..111027)
                     /locus_tag="ACHKW5_000100"
     CDS             complement(108931..111027)
                     /locus_tag="ACHKW5_000100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO7509563.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(111167..112507)
                     /gene="gntU"
                     /locus_tag="ACHKW5_000101"
     CDS             complement(111167..112507)
                     /gene="gntU"
                     /locus_tag="ACHKW5_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7509564.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPIIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(112511..113038)
                     /gene="gntK"
                     /locus_tag="ACHKW5_000102"
     CDS             complement(112511..113038)
                     /gene="gntK"
                     /locus_tag="ACHKW5_000102"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO7509565.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(113177..114172)
                     /gene="gntR"
                     /locus_tag="ACHKW5_000103"
     CDS             complement(113177..114172)
                     /gene="gntR"
                     /locus_tag="ACHKW5_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO7509566.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(114396..115091)
                     /gene="yhhW"
                     /locus_tag="ACHKW5_000104"
     CDS             complement(114396..115091)
                     /gene="yhhW"
                     /locus_tag="ACHKW5_000104"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO7509567.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(115214..116251)
                     /gene="yhhX"
                     /locus_tag="ACHKW5_000105"
     CDS             complement(115214..116251)
                     /gene="yhhX"
                     /locus_tag="ACHKW5_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7509568.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            116583..117071
                     /gene="yhhY"
                     /locus_tag="ACHKW5_000106"
     CDS             116583..117071
                     /gene="yhhY"
                     /locus_tag="ACHKW5_000106"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7509569.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            117308..>117552
                     /gene="tssD"
                     /locus_tag="ACHKW5_000107"
     CDS             117308..>117552
                     /gene="tssD"
                     /locus_tag="ACHKW5_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO7509570.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNE"
BASE COUNT        28473 a        31883 c        29461 g        27735 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agaaaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccaatcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcataggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatggac
     9361 caattgcaat tcctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccacca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 agcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaaattaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg caggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aagcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataat tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacaaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tacccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg gcgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactg aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gagctggaag ttgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttattttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaacgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgcg tatgtgggtc ttgggatcat cttgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 tggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacgcgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtggtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctcatggtag cggtctcctc tggcggcacc tctccggttc tggcacgcct
    27121 gttgcgcgaa aaacttgaat cactgctgcc gttgcatctg ggccaggtag cgaaatacgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgggtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgatcgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaaccaccg aacagttaat caacgaaccg cttgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaaccatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatatccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 ttgataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacata acatcatatg ttgttatatt catcatgcat tgtcatgtta ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccctac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29401 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29461 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29521 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29581 gccgatttcc gacgccctga cctggatccc cgcactcggc agcaccgctg ggatctttgt
    29641 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccaacc
    29701 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29761 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgcagg ggatcttctt
    29821 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29881 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29941 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    30001 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat
    30061 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30121 gtcgtaatgt tatctggaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30181 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30241 gtgcacacca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30301 tgcggatcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30361 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30421 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30481 ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc
    30541 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30601 cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg
    30661 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30721 cgcacctggg aagaaaaagg ccgccagcat ggtgaactgg ccagccagtg gccgatgatt
    30781 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30841 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30901 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30961 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    31021 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31081 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31141 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccgaat
    31201 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31261 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31321 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31381 gctggcgcag acgtatcaga tgccgattat cggctatacg ccagaaacca acggctatcc
    31441 gtataacatg atgctgggcg atgaacatat gcgtcgcgaa agcctcgaca tgatcaagct
    31501 ggcgatggat atggcaaaag agatgaacgc gggttatacg ctgatttccg cggcccacgc
    31561 gggctatctc acgccaccta atgttatctg gggacggctg gcagagaacc taagcgaact
    31621 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31681 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31741 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31801 ttatttcgac aaactgggtg ataaattgcg tcatctgcat attgtcgaca gcgacggggc
    31861 cagtgacaca cattacattc ccggcgaagg caaaatgccg ctgcgggagc tgatgcgcga
    31921 tattatcgac cggggctatg acggttactg cacggtggaa ctggtgacga tgtatatgaa
    31981 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    32041 tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32101 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32161 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32221 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32281 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32341 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32401 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32461 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32521 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32581 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32641 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32701 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32761 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32821 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32881 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32941 acggaccgct actctcatca actcctctac gctaccgtcc gccagcgact gctggatgat
    33001 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33061 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33121 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33181 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc gacgaaagag
    33241 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33301 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33361 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33421 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33481 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33541 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33601 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa ctattgataa taaaagacat
    33661 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcgttgaatt
    33721 ttacatcccg tacgttcctc tcaccctaac cctctcccca aaggggcgag gggaccgttc
    33781 agtacggaat ttagcttgac ggtgctatcc agcattaaat actggcaata ctctcacgca
    33841 aaatccgcag caccgtctct tcaccgctgc gattcgggtt aatgcgaatc gcacaatgtt
    33901 ctgattgtgg attcgcctgg cgaaacgttc cggaaaggcg ataaaagagc ggcgggattt
    33961 catatttcga ctctgcaccc accgggtaag gcaaggcacc gcgcttttgc gcctcttcca
    34021 gcactctggc ggcaatcggc tgatgaaact cgacaatcaa caccttcgac tgcgcattag
    34081 caatcaccgc gcttttcact tccggcaccg caccaccgtt aagcaaagcc agcaaccgtt
    34141 cagatacccc tgcctgcacc gcgtgcatca ctggcgcaaa caccagacca cgcaagactt
    34201 ccagcgcctg tgcgccctgg atctggctac cgccggagta aagcgtggcg cgaatacggt
    34261 tgataacatc agcatcgccg accactgcac caacaccctc tggcccaaat agcttgaagc
    34321 aggaaaatgt cgagatattc gcgccgcatt cacagccgat tcgcgccacc ttcatcaccg
    34381 catagttgtc atcggttaac gctggaacac ctgccgcgcg caacgttgcc agcacatctg
    34441 ccagcacgta gctgtcctgc ggctgctggc gcgtatgctg caccagcgcc gcatccggtt
    34501 gttgctcgtc gacgacctgc ttcagtgccg acaggtcatt gaaatcaaca gtaataagcg
    34561 tcagccccat ctgctcaata ataacccgtg tcgtcgggta aacaggcgcg tcatgcacca
    34621 gaagacgctg ccccggtttg agcaaagccg ccagcccggc gcgaatcgcg ccagtccccg
    34681 cgccctgcac cagcgccgca gcctgtgcgt gaaatgcatc agccagcacc tgttccacac
    34741 gctgggtaac gcgcggttga ttcagtcctg gcgttaagcc taaatcaccg ccagtaagaa
    34801 actcgctgcc ggggaaatgg cgacaaatgc tatccaccag cgcaaactgc ttttgctgcg
    34861 cctcaataat cgtcaggctt tgcagaggaa acgtcttcat aggtgccttc cttaagccgg
    34921 aacgaacagg ccaagccagt aaagaacatt cagcagaata ccggtgatca ttactgccac
    34981 gaccggtgcc gccattttct gtaccggacg gcccagggat tcgttgagga agtaaatggc
    35041 aaccgcgata gagaatccgg tataacccgc catcttaatt gctgcgaaaa tcgaaccgac
    35101 cagcagcgcc acttccatca gcatattcat ggcgttacgg atgttatccg acgcattacg
    35161 caccgacggg tagcgtccca gccatttgcc gatcgaacga agcagcaaga cttccgccga
    35221 aataaccact gcgcctaata ccgctgcaac catcggattc ggcgagagat agcccaccgc
    35281 ataaacaaag gtaaagcccg caactgcata cacacccgtt gctaaggcgg tggtggcaat
    35341 caacggtaca aaacccagcc cgcgcataaa ttctgccagt gccgcctggt tgatcagcgt
    35401 ttgcgattgt tccggcgtta cgcctgcgga gtacgctttc tccagagtga agatcgacac
    35461 ttcactgccg gcgaaaatct tcatgctggc aacggcggca atcaatgctc ccacgatggc
    35521 gatatagggt aagtttttga taatccgcga cgtgcgttct tcaaacaccg aaagcccgct
    35581 ggcatcaata tcattttcat cacgatgacg caggtcgtga gttatcgcga tccccagcag
    35641 catcaccatg ccaataaaga tttcgatgga ttcagggtta agatgtggga aatagcgcac
    35701 gactaccaca cgggtcatca gaacaaccac ggcggcgacc agactttgct tccagccaaa
    35761 ctggtagaaa atcgccacca gcgggaacag tgcaaaagca gaaaccaccg gcgagcttaa
    35821 ttccccgagg ctacctaata catccaccgg cagcgcggtc agcagctggt ttactggcaa
    35881 caggcaagta aggatcaata cgccccagat agcgccaaga ccaaacgcca tcaggctgtt
    35941 tatcgccagt acgccgagga tatcggtagg aagaaagagt aaccatgcgt tgagcagtcc
    36001 ggttttcagg gtaaaagaga tccccaccga ggccacaaaa ccaatgctca gaccaaaagc
    36061 gatactcccc gcctcgcgac ggttcatata gccttcaatc agttgcggca ggatcgggcg
    36121 gatgccgtca tgaaaaacag ccgccgagcg atgcgccaga agcgatgtca tacccgtcag
    36181 gcacgccacc acgataatct gaatatacag atccatagtc gtgcctttta tttcagatgg
    36241 ttaatcagca tcggaatcgc gtgttcgacg tgctcaacgg aaaggccaaa cgccactttt
    36301 ccttcagcga tcattttggc gatatgctcg tctttcgctt taatgcctgg tttggcaatg
    36361 gtgcaacttt tgttatagcc aatcaccgcg atggcaatcg acaatgccgc gcccgcaccg
    36421 gtattacacg caccgatgta gtaatccagt tgcccggatt tcactttcat tgccgcttcc
    36481 atgtcgttgt gaatgaaaac ttcaaaactg ccaggagccg tcgcttcaat ggttttttta
    36541 atctgctcac gctgtaagcc tgcaacgcca atctttttca tgagtctgtc cttattggaa
    36601 aaattgagag ggagtttcac gtaacatcac atccacatcg gcctgactga atcctgactg
    36661 gcgcaattgc ggaataaagg tggtcaataa atagtcatag ccataaccac cattggcttt
    36721 taaatgggag cggcgcgtaa tatccatcga cagcatgacg cggttcagca acccacggtc
    36781 acgtagcgcg tggagcatcg caatgcgctt ttcgtccggg tagtaactgt tcttaccaat
    36841 ggtgtcgaac tgcacgtacg cgccgagatc gatcatcttc aaaatgttgt cgaggttgtc
    36901 tttcagatcg cagtgaccaa cggtgacgcg cgaaagatca actccgtggg cttgcaacag
    36961 agccagttgt tccagcccca tggtgctgaa cgaagtatgc gtggagatcg ggcgtccggt
    37021 ctggttatgc gccagcgcag cggcaataaa caccttctct tccagcggcg taatcttccc
    37081 ttcgctagag ccgatctccg cgataatccc ggctttcagg tcggtgccat cgataccctg
    37141 ttcaatttca tcgaccatct cctgcgccag ttcctgcacg ctgcgggtcg ccacatgctc
    37201 cgggaaaaaa gcgtcctggt aataaccggt gcaggccacc acgttgatcc ccgtctcgcg
    37261 cattacatcg agcataaatt gcgcattgcg ccccatgtaa cggttggtca tctcaatcac
    37321 attacgcacg ccccgggtca tcaggtcgtt catctcctgg caaatgaacg catactgatc
    37381 aaggcggcag tccacgttgt ttttaaagcc ggagagatca atatgcagat gctcatgggc
    37441 cagggtgtaa cccgtcggat caaaactcat agggtgtctc ctgcaaaccg gagggaggaa
    37501 agaaaagagc gaccattttg tggcggtggc gcacggaaaa attcacacac ggtagccccc
    37561 acatctgaaa gcgtggtgcg cacaccgagc tgcgtagcta tcatcccttg ctgataaacc
    37621 agcactggca ccacttcgcg ggtatggtgg ctgtgaccaa tggtcggatc gttaccgtga
    37681 tccgccatca cgaccaggca atcatctggc tgcatcgcct caacaagccg ggcaaggtta
    37741 cggtcaacga cctgcaaacg ttcggcataa cgtgcgacgt cttctgcatg accagcgagg
    37801 tcggtttcct ggatattggt gcaaataaac gccgtcggat gcgtgttaaa ttcgtcaagg
    37861 gtgatatcca taatccgctg gctatccacc aggttttgcc agctcacgcc ataaggattg
    37921 ctgacgatat ctgccacttt acctaccagc acggttggca cgcccgcctc atacagtttt
    37981 tgcggcacct gcactttttc atcaacaccg tagcccatat gcacgacctg gaaaccgttg
    38041 tcataagcgc cagaacgcgg cgcattgatg ccaataaagc gcccttcttt gctttctgcg
    38101 gcatcgagaa tacgctgact gtcggttaac aagccgccaa atgtaatgac ccgaccgacc
    38161 tgtacctgct cacgcacgat acgaccaatt ttgattgcgt cgtcaaaaga gatcacagag
    38221 agattggcgg taatgttata gacttggcct aaatccgcct cgagattatc gccaatcgca
    38281 accgcctgat tgacccacag aaattgcaga tcatcgccac ggcgctccac ctgccagcca
    38341 gcggaaacta atgcctgctc aacacggtca atcacatcgc gaaaaggcat ccgcagcggc
    38401 ggtaacgggc gcgtgcctaa aatttcctga tgccccataa aggtatcgcc accttcatgt
    38461 tgcagctctg ccacgcccca ggttgcggaa tctgacggct gcatatcgcc tggcgcataa
    38521 cccaatgcgt tgattagccc cagcttctcc agcgttggta gctgcaaatg cggcaactgg
    38581 ctcaggatgt gaccacaggt attcgctccc gcatcttgcg gacgcaccag cgtgacatct
    38641 ttcattgccc ctacgccaaa gctatcaatc actaacacca caaatcgcgc cattacgccc
    38701 ccagagagtt tccaagactg tcataacgac caacgatttt cggttcgcca cggtgaatac
    38761 cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcggaaa cagagcacca
    38821 ctgcactgct taccggaaac tcgcctgcca gcgacagggt gtagtcgata ctgctgtcat
    38881 ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac accaacgcat
    38941 gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg ccacggaaat
    39001 gatgggagat ttcactcaac cagagcatcg cgatacgttc agactgatcg ccctgctggt
    39061 ttgccggaat agtgcccgtc agcgcatgac cgggttcggc atgagtcaca ccgtattgcg
    39121 ccagtaatgg cagcgaagtg cagctggtcg ctgaaggcgc gttcagttgc tcaatcacaa
    39181 taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc ggtgtcggca
    39241 aaactttccc ggcagcctca tcccaaagca ggcaagggaa atgggtaagt ccggctaaat
    39301 gcagccccgg cagtttctta atttcagcaa ccacctcgtg cagcgaatgc tgtacaaaac
    39361 cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc agcacagact
    39421 ggattcgccc ggccttcacc gccgccgcag aaacttcccg cgctttgtcg agagtaaaca
    39481 cggtgatgac gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa gggatttgta
    39541 ccagatgccc ctgatgcgcc accggcaaac cagcgcggcg catgactcgc gcctctttgt
    39601 aatccaccgc cacaatgccg ctgtagccca acgccagcag tttttccgcc agccatgggt
    39661 tacgaccgaa ttgtttggtc atcagataca gttcaatgcc gtaaagccgc gccgtctcaa
    39721 tcagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg tccggggcga
    39781 ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc tggcgtttca
    39841 atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata ttcatttttt
    39901 tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt cgttagcagc
    39961 catccataat ccataaaggt tggccagcaa atagccttct tcacacgggt taacttccag
    40021 tgcgaattcc ttcaacaata cctgatgcaa ttgcacaaca gcttgccagt ggctggattg
    40081 cgccagctct gccagcaatt cgttatccag cggctctatt tcttcaccac ggcgactgcg
    40141 catcagtgca ctcgccatat gtgtaatcgc catcgttcct tgctcactgc gcactggcag
    40201 atggcactct gtttccagta cgttgacgac ctgcatcatg cctttgcaga tgtccttatc
    40261 aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg cctttcgttt
    40321 aataactcgg ttcctgttcc ccactcacca cgcgttgttg atgggcgagc agggcgtgtt
    40381 tatctacaac ggcacgcagc agttgttgca ttgggtaatc atcggctcgc gtcaggacca
    40441 cggcttcggt ggcctgtaaa aatcgcggat cgtctgtgag cggcgtcgcc tctaatccca
    40501 gcatggtcag ttcgttttcc gccaccacgt tccagataac cgcatcgaca tcgcctttaa
    40561 caatgcgttg taaactctcg tgataagaga gatcgacacg ttccacatca ctatcgccaa
    40621 aaaagacatc ggtcatgatt ttctgatccg ccgaacggtt atccagcccc acgcgcttca
    40681 cgtttgcgga ctcgccttta cggcaaatca actggtgctc gccaacgtag gtgtgcggcc
    40741 ccaactccag cgcgaggcaa aacccttttt gcgtgagata actttccgcc gccagtcggg
    40801 aaaccaccgc catgtcatac acgccgttaa gtaaacactc cacgcgaata tccgcgccgc
    40861 gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg ctcgctaaac
    40921 cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg atatccacat
    40981 gactcagcaa tgctttgtta tccatctcga ccagataact gccattgcgc ccacggcgtt
    41041 caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc aggccaacgg
    41101 atgaacggca ttcagttgcc agctcatcta tggttttcaa cctattaccg catttttcac
    41161 ccaacaaata acgggccagc gtcgtgataa cgacgccttc ttttttgata aacgttcgac
    41221 gcataatata ttttcaataa aatgaatgtt tatatcttca ttaaaatgaa gataagtggc
    41281 aactgtgaga tgtggaaacg gcgaggcgct tcacattttt tcggattatt gcaaatgagg
    41341 ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat tcgacaatgc
    41401 gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc ctctccccaa
    41461 aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa accaatcgct
    41521 tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc gccgtctttc
    41581 atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc ctgcaattca
    41641 gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata catcttgcct
    41701 tcgaactgtt tttccagttc tggaatgctc tggcccgtta ccgcagaaag gatatccagc
    41761 aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg ctcgtcggag
    41821 tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag caggccgata
    41881 acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag cagcgacatt
    41941 acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc gccatacagc
    42001 gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg gtcttcaccc
    42061 accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac cggatagtca
    42121 aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa ctgggtcatg
    42181 cggctcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg cgcatgttct
    42241 ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca agccagatac
    42301 aaggccagcg tatccagcgt cgctttacgc agcttctgtg catcctggcg cacggtgatc
    42361 gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt tacccactga
    42421 cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc gccactaaaa
    42481 acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc ctaatgcggg
    42541 cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga tagcctcgcc
    42601 gtagttatat ccgtaggtta agccaactga tgggcaacct gccgcttttg ccgcctgaat
    42661 atcattgcgt gagtcgccga caaacagcat ctgttgtggg gcaattccca tccgctcagc
    42721 caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc cgccgataac
    42781 cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga acggcgtcgg
    42841 tttgttggtg accaggccta gcggcaggcc tttagcctgc aacgcgccca acgtatcggc
    42901 aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc gatcgaacag
    42961 tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg gcggtttacc
    43021 cattgtttta cgctgagtcg cacgttcctg acgcgcccag gtcaatgcgc gctccatcag
    43081 aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga cgggcaactc
    43141 cagcgcatac agcgccatat ctaccgcagc agcaagaccg ggagcactgt cgaccagcgt
    43201 accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga cttacctttg
    43261 ccagttcact gcgcatttca tcaatgactt ttttgtagtc tggctggtcg aagattgccg
    43321 aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg ttcaccttca
    43381 cgccaccgtc tacttccaga cgaatgtcaa agccagactc gtcgatacga cgacgtactt
    43441 cgcgcagttt atccagtgtt tgaggaatga acgactgacc gccgaaacca ggattgacgg
    43501 acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc agcggtgtcg
    43561 ccgggttaaa taccagtccc gctttacagc cattttcttt aatcagttgc agcgtgcggt
    43621 caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg gcagcgaaat
    43681 caggcacaat gcgatcgaca ggtttcacca tcaggtgtac gtcgataggg gcggtaatgc
    43741 catagttacg caaggatttc agcaccattg gcccaatcgt cagattggga acatagtggt
    43801 tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt gcggtatctt
    43861 cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac tgtttcatcc
    43921 gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag
    43981 cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac
    44041 gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat
    44101 cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat gcgcttgttg
    44161 ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggtgc
    44221 ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgcgccatgc tatcggcgta
    44281 agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc
    44341 cgggaaatag ggttttttgt agcggccaaa cggcacatta aactcaccgc gcagattgta
    44401 acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa
    44461 cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg cgcaatttgt
    44521 ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat
    44581 gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt
    44641 gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc ccttgggcaa
    44701 atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca aaaaagcgcg
    44761 atttttcttc atgctgacta actaattaca ccttctccgg ctgtggagaa agctccgaca
    44821 gcatccagcg cttcaggcaa ctcccgcaac gataaaccat tgcgggagat aaccttgatt
    44881 acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg gcctggacat
    44941 ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac acgccagaaa
    45001 ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag tttttcagat
    45061 tctctttctt cgcccaaccg ttcaggttgc cgtagttaga ggaactgctc agctgcagag
    45121 tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc ttccctccag
    45181 cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc gtagtcgccg
    45241 tctcttttgt tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc acaggggcag
    45301 ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg ctccgttttc acggttgctt
    45361 gcggtttttt cggttcaatc actgcctgct ggcgagctgg acgcgtagtg gccggacgtt
    45421 cagcggtttg cgttttcgcc gtttcacggg atgcattgcc attgcgaaca ggcgcgacag
    45481 tcgcgggttc ggtcggcaat gtggaattga ccgccacatt gttcaactgt tgctgatttt
    45541 gtggttgggt cagcgcattg ttcaggtcac cctgcacttc aacacgttgt tgaccatccg
    45601 ttgccgccgg ggtttgccct tgagtcggcg tagaagagat cggcggcaga gaaacatcct
    45661 gctgagtatt ttccgcagac gtggttcccg gcgctggctg cacgccattg gcctgatcgg
    45721 tcgcattgcc agcaagatca atactcttct cgccagacgc ggtttgatcg ctggaagagg
    45781 tcgagggggc ttttagcgca gaaccgatac cgatgatcaa cagcagtaga accagaatgc
    45841 cgacgcccat catcatatac tgacgagaag cgggtttact ggctgctttt ttgcgcttac
    45901 gcggacgacg ctctacgcgc tcttcatcca cggtttcatc ttcggattcg tcaatttctt
    45961 cttcgatttc cggttcctca ttgcgctctt tttgcgcacg agtcggacgg cgatcgtcag
    46021 tgtcatcaag ttcaatatca tcaaaattga tctgcggttc gccacgttca gtacgctcag
    46081 aacgttcaga agattgacga gaacgaccag tacgacgatc gctgggatcg ggtttcagct
    46141 cgtcttctgg tttgaattca tccatttaac accccactaa aaggttaatg cttaccacgt
    46201 tgcaattaac ctgaagctaa tagaccgctt gataagcggc ctgacctttc ttgttgttac
    46261 gctgattgac aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc gctgcgaact
    46321 tcactcttac caattgccaa cggaagaatt aagcgcatct ctcccgcaag gactttcttg
    46381 tcacgcagca tatgcggtaa atacgcctgc gcggacattt cgcgcggccc attgaccggt
    46441 aacccagccc gcgtgagcag ggttataata cgctgcgttt cggcagaact aaactgcccg
    46501 agacgttccg acgtccgcgc cgccatcacc atacccgcag cgaccgcttc accatgtaac
    46561 caattgccat accccatttc agcttcaatg gcatgaccaa aggtgtgtcc cagattcagt
    46621 aaagcacgta acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt cagttcacaa
    46681 caacggcgaa tacagtacgc cattgccgga ccgtccagac gcaacaacgc atccagattc
    46741 tcttccagcc agttaaaaaa cgcaccgtca agaataatgc cgtatttgat gacttctgcc
    46801 agccccgacg ctaactcacg cgggggaagc gttttcagac agtcgagatc caccaccact
    46861 gaagcaggct ggtagaacgc gccaatcatg tttttaccga ggggatggtt gaccgcagtt
    46921 ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg tcgggacttg aatgaaacga
    46981 acaccgcgct gataactcgc cgccgcgaag ccggtcagat cgcccactac gccgccgcca
    47041 agcgccacca gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt aaagacggta
    47101 tcgagtacag ccaggctttt atactgctcg ccgtcaggga ggataacgct atcgacgtta
    47161 acacccgcct gttcaagtac gccgcggacc ttatcgagat acagaggagc cagggtttcg
    47221 ttggtgacca acatgacctg ctcgcccgat ttcagcggta agaatgaagc tggttcatta
    47281 aacaaaccag atgcgatggt aattgggtaa ctacgttccc cgagagtaac gacaatcctc
    47341 tccataacgc gacatccacc ttaattactg tacccgcaga cgagtgtata taaagccaga
    47401 attagttgct ttccagcatg tgaataatct ggtttgcaac cactttagcg ctttgatcat
    47461 cagtacgaat ggtcacgtcg gcaatctctt catacagcgg attgcgttca ttggccaacg
    47521 cttccagaac ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt ttatcacgct
    47581 gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata aacgacaacg ccacgagcgg
    47641 aaagacggtt acgcgtttca cgggatttca cagagccgcc gccagtagcc agcacaatac
    47701 cctgtttctc ggtcaactca ttgatgacct tttcttcgcg atcgcggaag ccttcttcgc
    47761 cttctaaatc gaaaacccag cccacatcag ctccggttcg tttctcaatc tcttgatcgg
    47821 aatcgtaaaa ttccatattg agttgttgag ctaactggcg cccaatagtg ctttttccgg
    47881 cacccatagg cccaaccaga aagatattgc gtttctctgc cattttttcg gtactactaa
    47941 gactattcgt taatgataaa cccgcttcgc tcagagagcg ccgcaggaca tgaactgaaa
    48001 cctcataaga tattgcgaga gtcagactga aaattatctc aatactccag cgggtttggc
    48061 aactgaataa atcacccatc cccctgcata tctggtcgct gcaagcgcgc tgccttgcta
    48121 ccaccgctct ggcgataaat caccgggtaa gattagcgta aaaaagacag caaaatgccg
    48181 cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac tctcaaatcg
    48241 gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcataaa
    48301 aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc
    48361 actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga
    48421 atgtcgccaa gcaacggtac gctatcctga cccgatttat ttttacgggt aaaaatgccg
    48481 cccagcgcca acgtttctcc gcttttgacc tcgacctgcg tttcgatctc ctgcttatca
    48541 atcgccagca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg
    48601 tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtgacctc catccccagg
    48661 acggcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac ctgatatgga
    48721 atttcgctcc cctgtttaat gctggcaggc tgaagatgtg aggccagcag acgcggactg
    48781 gcgataatat ccagctgctg tttttgttcg agcgcagaaa gctcaagatc cagcaagcgt
    48841 ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg
    48901 ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc cagcgtccat
    48961 ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca
    49021 acctgcccga ccggcagatc catttgcgct acccactgtt caagcgcgct taacgccgtt
    49081 ttgttatctc gtagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggca
    49141 ctcagtagct tctcccccgc tttcgccagt tctcccgcgt cggcgtattg cagggttata
    49201 ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcctc ctggcgggcg
    49261 atattgttat tctgccaggc aatggaatgc actgagagaa tgttgtcttc ctgccgcgtt
    49321 atcagtccgg cgcttttcac tacagtttgt agtgcctgct tccagggaac atctgttaga
    49381 tgtaacgaca ccgtaccgct gacgtctggc gacacgacca ggttcaactt ctcctgttca
    49441 gccagcgcct gcaacacctg agctaccgga acgtcatcca ccatcagcgt cactttttgc
    49501 ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc
    49561 atttgtatct ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg tcccggttcc
    49621 cagcgttagt gcgtctggcg tcagctgtaa aattgtccag ccgttttcca gcacatcgtt
    49681 ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt acaccgatga tgcgctcgcc
    49741 tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc
    49801 cggcggttta aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa
    49861 ccagcgttta accctcatgc ggcgtctcca gttgtagcgt gaacaaaaga tcatcacctt
    49921 ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg
    49981 acggcactgc ttcccacagc gttttcaacg ccaactcacc tccctgcgcg gatggatgcc
    50041 aggaaaccag ttgcgcgccg gataactgaa aatccagagg cgaaaagggc agcgtttttt
    50101 cctcgctgaa gggagtggtg tctaccaggc gatacagtgc ggcccactgc tggtgatgac
    50161 ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgat agaaaaatga
    50221 gcgtaactaa catcagcaac cagaatgccc agcaaagctg gcggaggcgg ggtgatgtgg
    50281 cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat
    50341 tgccagcgtc cctgcgcatc ctgctgcgtg gccccccgct gattgagatg aaaagaagca
    50401 tcctggcgaa gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt aagccccttg
    50461 atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgttc aggtaaaaga
    50521 gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgcctt
    50581 tgcaaacgct gctcacgaat ttgttggcgc tccagcaaac gtggtttcgt tatctgtaag
    50641 ctgtgggcga gttgttgttc cccctgaagc aaaacggcgt ctacgcgagc ttcggcgctg
    50701 cttgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc
    50761 agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt
    50821 ggcgggttca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca aagtctccac
    50881 ctgacggcgg tagcggcggc tgacgaacag aaacggcctc ccagggatca aatccccctt
    50941 cgccacatat cgcgacgctt tcgggatcaa cggataacgc tgccgccagc tccttcgcgc
    51001 tagtcatccc caccgccagt ttgcgccccc agcgatagcg tgtcgcccac agccactgtt
    51061 cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta
    51121 atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct gccagagtaa
    51181 gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataagcgggg ctgagtgagt
    51241 cttcgctata atcgaagcgc agggagtccg gatccatatc cagctcgcgg gccatcgtcc
    51301 ctgacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgc ggaaatgacc
    51361 gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc
    51421 gactccaagg taacaacgtt ttagccagct gctgcgcatc aacaatccgc ccatctttga
    51481 taatgtcgtt ctccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc
    51541 gtacgatcgc aaccgctacc gcttcttgct gttgtaaatg caaaccaatt tgccagatct
    51601 taaatgccat tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc
    51661 tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg
    51721 aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg
    51781 ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggcg
    51841 acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg
    51901 attgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccaccggag
    51961 atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac
    52021 ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg
    52081 gcgagtacca ttacccagca gctggcgaga aatttcttcc tcagtccaga acgcacgctg
    52141 atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac
    52201 gagatcctcg agctttatct gaacaagatt tacctcggtt accgcgccta tggtgtcggt
    52261 gctgcggcac aagtctattt cggaaaaacg gtcgaccaac tgacgctgaa cgaaatggcg
    52321 gtgatagccg ggctgccgaa agcgccttcc accttcaacc cgctctactc aatggatcgt
    52381 gccgtcgcgc ggcgtaacgt cgtactgtcg cggatgctgg atgaagggta tatcacccaa
    52441 caacagttcg atcagacacg cactgaggcg attaacgcta actatcacgc gccggagatt
    52501 gctttctctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc
    52561 gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag
    52621 gccgcacagc aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc
    52681 ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc
    52741 gatacgctga aggcgctgcc aacctatggt ccgctgctgc ctgccgcagt caccagcgcc
    52801 aatcctcagc aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa
    52861 ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc aaggaccgac gccgcgtaaa
    52921 gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg
    52981 tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc gcaaaacggt
    53041 gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc
    53101 caggcactgc gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat
    53161 aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt
    53221 gccggttctg actggcagcc gaagaactca ccaccgcagt atgctggtcc aattcgctta
    53281 cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc
    53341 gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac
    53401 accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac
    53461 gcggtcatgg cgaacggtgg cttcctggtg gacccgtggt ttatcagcaa aattgaaaac
    53521 gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt
    53581 ccggtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat
    53641 gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca
    53701 aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact
    53761 ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga accaggctgg
    53821 cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc
    53881 ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc
    53941 tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga
    54001 gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg
    54061 gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgcctccg
    54121 ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc
    54181 cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga
    54241 acgaccatta tcgataatgg tgaggcacag gaattgttct gattaaaaag gcgcttcggc
    54301 gccttttcag ttggctgaca aagtgcattt gtttatgccg gatgcgggtg aacgcgttat
    54361 ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa acccataagc ctgataagcg
    54421 ttgtgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc
    54481 acgaggaaca gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc
    54541 atcatatgcg ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc
    54601 gattccgggt agagatcttg cgcgaccacg atattcattt tgctggaaaa gtaagacggt
    54661 gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt
    54721 agctcgcggt tagcggcttc gtagacgctt tcacccggat caattaatcc tttcgaaaaa
    54781 cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc
    54841 acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc
    54901 cgcacgccat tgctgaactc cagatccacg ctctcgacgg taaacagtcg ggaacgggct
    54961 acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg
    55021 ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg
    55081 ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag
    55141 gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc
    55201 ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc
    55261 gcagaatcat gtattgtgtc tggtgcgact gaccacgcct gacagactaa gtaagatggg
    55321 gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg
    55381 ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg
    55441 gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag
    55501 agcctgacgc aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatctct
    55561 ctggcgctga atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttac
    55621 ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac
    55681 ctgccccctt tctgggaaca atacatcaac gatgagaata ccgttgaact gatttatacc
    55741 gattcgctgc cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa
    55801 acgcgcggct atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag
    55861 agtgagcaaa tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt
    55921 cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc
    55981 tgcctgatta ccccagatgt atttgttccg tggctggcag gcggcgcatt actgctgctg
    56041 ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg agaaattcat
    56101 tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc
    56161 aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt
    56221 gctcaggatc tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cgtagtgcgt
    56281 caggggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg
    56341 cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt attctggatc
    56401 ccgctggata tgccgctgaa attcactctc tcatggatga aaggcgcgca gaccattgaa
    56461 gccaccagcg taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt
    56521 agcggtacgg gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca
    56581 ccttttttac cgtttgactg ctcgcagatc atctggaatg acgcccgctc attaccatta
    56641 ccagaatctg aactggtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg
    56701 caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc gattcaaaaa
    56761 tccggcatgg tattgcttga tgattttggc gacattgtac tgaagacagc ggatttatgt
    56821 tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa
    56881 gactgggacg cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta
    56941 ttacgcccgg tgagtgcaga atcgctggat aacctggtgg caacctccac cgcgccgttt
    57001 atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc
    57061 ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt
    57121 tacgactacc cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg
    57181 catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc caatggtacg
    57241 cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc
    57301 acattgctgc tactgacgat gctgggtagc gccatttaca atggcgtaca ggcctggcgt
    57361 cgttaccagc gtcatcgcac tcgcatgatg gagattcagg cctattatga aagctgcctg
    57421 aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt
    57481 tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat
    57541 tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc
    57601 cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcca aaaacggggt
    57661 tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct
    57721 aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac
    57781 caccgagatg ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc ttgaggcact
    57841 gaaagccagc ggtaagcagc gaattttgct caccaatgcg catccgcaca acctggcggt
    57901 aaaacttgag cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt
    57961 tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa
    58021 agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt
    58081 tggtattcgt tactgcctcg gcgtgactaa tcctgattcc gggattgccg agaaacagta
    58141 tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag
    58201 acgccatgaa agagaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc
    58261 gtttttataa aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca
    58321 acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc
    58381 agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca
    58441 gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg
    58501 cgatggcacg taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa
    58561 aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa
    58621 gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aattttgccg
    58681 ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag aaccacgatt
    58741 atccgcagcc cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg
    58801 ctacgctgaa gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc
    58861 tggcggttat taacggtaac aataaccagc aaatgcgcgg tgtggcgcgc gtgcagggcg
    58921 aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca
    58981 ttaccccgag cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt gataccctgg
    59041 cggcctgcct ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc
    59101 gcaccggcga cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg
    59161 cgcaaaatgc ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa
    59221 ccgaagaact gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag
    59281 aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt
    59341 gcgccgatgc gctgaaaacg ctgcctgatg aagaagttga tagcatcctg gcggaagatg
    59401 gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata
    59461 ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc
    59521 cggcagagag ggctctgccg gattttacga cgtaatggcc tgatgtgctg cgcttaccag
    59581 gcctgtgaaa taaatcgcaa attattgttg gtcagataca gcgtcgtatc cgttaaaaga
    59641 gttgtcactt acttaagttt atttccagcg tttcaccatc taccgtcaga tcgcgccacg
    59701 ctttcggcct cgtccccagt tttccatcct tcgcagctgt gagtaatttt tctttcgtta
    59761 tttcgttcgg aagtaaactc atatcaagcg cgtcccattc ttttctgtct gagtcaaagc
    59821 catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc tgaccatcac
    59881 tattcagatc ttgctcgacc aggacgcagg cgtctttttc aatacaggtc atcacgttat
    59941 agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaacagaa ccaggggcaa
    60001 ttaacacatt ttcggctaat actttttccg atacctgttc ctgaagatgt tgctctccat
    60061 ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg gcatcgcttt
    60121 tcagcgactc aagcgcagca cgtccatagc gaccgctctg ctcgagcatg tagatagtta
    60181 cctggtctga cgtgtttttg ccgctctgat aacgcgccat atggctgttc acgctaatgc
    60241 gcatactgtc cagcaccggc gaattaagaa gcaccagtat caccaacacc aataacgaaa
    60301 ccgcaaggtt cactttgccc tgaagaacaa cgggattttg ccctttacgc cagacgatgc
    60361 tgacaaaata ccccagcgac cacaccagta acaccagcac cgccagcacg ccctgcaagc
    60421 ggtcgacagt ccagccgtac tgagcgaccc gcagccataa cgcccaggcg gcgacgaaca
    60481 catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaacggc cctgtccagg
    60541 gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc aaaaaggcca
    60601 gcgttaacag caacccggcg gcggagatgt ggcgagaaat cgcgctcagg cccgtaaacg
    60661 gcagagtgat gataaacatc agggttagca atgatactaa cggcagcaac cccgtggcga
    60721 ttaacgtgaa caacttttga atagagtcga ttaaacgtga ctgtgttcgc gcgaggatca
    60781 ccgccagcgc ggtaaccaga cctaacgtga gataaataaa ccagtcggtt gcaaaaaaga
    60841 gcgtattgaa aaacgtgatg ccaacaagtt taaacaactc actccacagt aaaagcacca
    60901 gccacgtcaa gccattggcg agaaaaatca ccagtaatat aagtacgtta tgccatactg
    60961 actggtaaaa atagcgatag cggctactgt cattgcggat gcgaaggctt tgttgtatcc
    61021 acggtagcaa caacattgcc atcagcagca gatagcaacc aaaatcccaa agcgcctttt
    61081 cagctctcca ggggttcatg ccatcagttt gccacttcag ccagccgctc atccccagcg
    61141 tggcaataaa caccagcgcc agccatcccc aaaggcgctt ctgtttaaaa gagatcacgg
    61201 agaagagtaa aacggatgaa aacgcaacgg tcgcaggcac gccataaaca atccaactgt
    61261 gattttttcc ggcaagccag gcgatcaaca ggtagcaaac cagcccctga agtaatccgg
    61321 tagcaatcat cccccagcgt gttgccggtg aaagttcaac gttatccatg ttggttatcc
    61381 agaatcaaaa gtgggttaat tatcgcatcc gggcagtagt attttgcttt tttcagaaaa
    61441 taatcaaaaa aagttagcgt ggtgaatcga tactttaccg attgaatttg catcaatttc
    61501 attaaggaat gcgattccac tcacaatatt cccgccatat aaactaagat ttaacctttt
    61561 gagaacattt tccacaccta aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag
    61621 gaatcacgga gttttttgtc aaatatgaat ttctccagat acgtaaatct atgagccttg
    61681 tcgcggttaa cacccccaaa aagactttac tattcaggca atacatattg gctaaggagc
    61741 agtgaaatgc gcgttaacaa tggtttgacc ccgcaagaac tcgaggctta tggtatcagt
    61801 gacgtacatg atatcgttta caacccaagc tacgacctgc tgtatcagga agagctcgat
    61861 ccgagcctga caggttatga gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat
    61921 accgggatct tcaccggtcg ttcaccaaaa gataagtata tcgtccgtga cgataccact
    61981 cgcgatactt tctggtgggc agacaaaggc aaaggtaaga acgacaacaa acctctctct
    62041 ccggaaacct ggcagcatct gaaaggcctg gtgaccaggc agctttccgg caaacgtctg
    62101 ttcgttgtcg acgctttctg tggtgcgaac ccggatactc gtctttccgt ccgtttcatc
    62161 accgaagtgg cctggcaggc gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa
    62221 gagctggcag gtttcaaacc agactttatc gttatgaacg gcgcgaagtg cactaacccg
    62281 cagtggaaag aacagggtct gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc
    62341 atgcagctga ttggcggcac ctggtacggc ggcgaaatga agaaagggat gttctcgatg
    62401 atgaactacc tgctgccgct gaaaggtatc gcttctatgc actgctccgc caacgttggt
    62461 gagaaaggcg atgttgcggt gttcttcggc ctttccggca ccggtaaaac caccctttcc
    62521 accgacccga aacgtcgcct gattggtgat gacgagcacg gctgggacga tgacggcgtg
    62581 tttaacttcg aaggcggctg ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct
    62641 gaaatctaca acgctatccg tcgcgatgcg ctgctggaaa acgtcaccgt gcgtgaagat
    62701 ggcactatcg actttgatga tggttcaaaa accgagaaca cccgcgtttc ttatccgatc
    62761 tatcacatcg ataacattgt taagccggtt tccaaagcgg gccacgcgac taaggttatc
    62821 ttcctgactg ctgatgcttt cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa
    62881 acccagtatc acttcctctc tggcttcacc gccaaactgg ccggtactga gcgtggcatc
    62941 accgaaccga cgccaacctt ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg
    63001 actcagtacg cagaagtgct ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg
    63061 gttaacactg gctggaacgg cactggcaaa cgtatctcga ttaaagatac ccgcgccatt
    63121 atcgacgcca tcctcaacgg ttcgctggat aatgcagaaa ccttcactct gccgatgttt
    63181 aacctggcga tcccaaccga actgccgggc gtagacacga agattctcga tccgcgtaac
    63241 acctacgctt ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc
    63301 gacaacttcg ataaatacac cgacacccct gcgggtgccg cgctggtagc ggctggtccg
    63361 aaactgtaat gatttgaagc tggagaatat ctatccagta tcttatagaa agcaaaacgg
    63421 gaggcacctt cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac
    63481 cggcactggc agccaggcgc gaatggaaag cccgccccgc tcgctggtgc caagctccag
    63541 catcccgtta tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct
    63601 aatggtgcgc gcactgtcgc cgcggacaaa cggctggaac aggtgcttac gttgttccgg
    63661 cgcaattccc ggaccgtcat cttccacctg gaaccaggcg cgattcggct ccgttccgct
    63721 gctgactttg atccagccat tgccataacg ggcggcgttg accaccatat tcgccaccgc
    63781 gcgtttgatc gacagcgggt gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc
    63841 aatttcccgc tcatagccac tttcggcagc aatcacctca ccgagtactg cattaagatc
    63901 cgccatttcc atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat
    63961 ggcgttgcac tcttcgatat ctttattgat cgattctgcc agatagccat cctgctcgct
    64021 catcatctca gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc
    64081 cgccatcagc agcgtgcggt catccgccag ttgcttaaca ccagccgcca tatggttaaa
    64141 ggcacgggta acggaacgca cctccgaagc gccatactca cgcagcggcg gcggaataat
    64201 ccctttacca acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt tctggatacg
    64261 aataaacagc cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag
    64321 cggagagaaa tcgccctgat gaatttcggt cagcggcacg cgtacccaga tattgggcga
    64381 cagccaggtt ttcagccaga cgacaggcga acttttgttg acctcaacgc gcacttccgt
    64441 cgggccgccc agttgctgtg ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg
    64501 cagacctgcc tcttcggcag cctcgttgga gtagagagag atccccagct cacggtagat
    64561 ctcccgacgg aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc
    64621 ggtcatcaac atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa
    64681 aatcgcgaag ttcagcacca ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac
    64741 gatgagcaat aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct
    64801 ttagagccgt ccggtacaaa gacgtagccc aggccccaaa cggtctgaat gtaacgcgga
    64861 tgcgctggat cttcttctac catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt
    64921 tccattgcgg aatattcacg accacgggca aggttcatca gcttatcgcg ggagagcggc
    64981 tcacgcggat ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc
    65041 ggctcatctt cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca
    65101 attaccgcct cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc
    65161 gcacgaatac gggccagcag ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg
    65221 ccaatctcca ggcctacgat acggtccact tcttcccctt tcgccgtcac cataatgatc
    65281 ggcatcgggt tgctctgact acgaagacgt cggcaaatcg acaagccatc ttcaccaggt
    65341 aacattaaat ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt
    65401 tctgcattag cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc
    65461 gcacgcaggc gcatgtcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt
    65521 actcccaaag gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca
    65581 ccagctaaat ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct
    65641 taagtataca atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc
    65701 caatgtgcgc attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac
    65761 ccgggaaggg tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc
    65821 ggaggtcaca aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga
    65881 ctatcagtat aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa
    65941 atgcatggaa aatctcaaaa tcgtcgatta ctcccctcag caggaaggca aagtcttttt
    66001 tggcgcgtgg gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg
    66061 ctacgatgaa atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc
    66121 attgctgaaa aaagaagtcg gcgatctggc tgtggtgaat actcctgccg gggaagcgag
    66181 ctggtatgtt aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg
    66241 atggctggca tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag
    66301 atgaactcaa accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc
    66361 gcgcccggaa caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt
    66421 tatcgcacgt tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct
    66481 ggaaacgcgt ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc
    66541 catttccgag caaggcaaac tcaccgatga tctggcgaag gccatcaacg ccaccctaag
    66601 caaaaccgaa ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca
    66661 aatcgccatt gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca
    66721 cacgccagaa gtcgcggctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa
    66781 agccgcgctg gacggcgcgc gctatatcct gatggaacgt tttgccgaag atgccgcgct
    66841 gctggcgaaa gtgcgtgatt atctgtggaa gaacgcgcat ctggtctcaa ccgtggtgag
    66901 cggtaaagaa gaggaagggg caaaattccg cgactatttc gatcatcacg aaccgttgtc
    66961 cacggtgcct tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtactcca
    67021 gctttcgctg aatgccgatc cgcagttcga tgagccgccc aaagagagct attgcgagca
    67081 aatcatcatg gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa
    67141 aggcgtagtg agctggacct ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat
    67201 gggtaccgtg cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca
    67261 cgatctgctg atggcggccc ctgccggact gcgtgcaacg atgggcctcg atccgggtct
    67321 gcgtactggg gtaaaagtgg cggtggtcga tgccactggc aaactggtag cgaccgatac
    67381 catttacccg cacaccggac aagccgcaaa agcagcgatg actgttgctg cgctgtgtga
    67441 aaagcataac gttgaactgg tggcgatcgg caacggtaca gcttcccgcg aaaccgaacg
    67501 tttctatctc gacgtgcaga agcagttccc gaaagtgacc gcacagaaag tgatcgtcag
    67561 cgaagctggc gcgtcggttt actcagcttc cgagctggca gcgcaggagt tcccggatct
    67621 cgacgtttcg ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc cgctggcgga
    67681 gctggtgaaa atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca
    67741 gacgcaactg gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt
    67801 cgatctcaac accgcttctg ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat
    67861 ggcgcaaaac atcgtggcct ggcgcgatga gaacggccag ttcaagaacc gtcagcaact
    67921 gctgaaagtc agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat
    67981 taaccacggt gataacccgc tggacgcctc taccgttcac ccggaagcct atccggtggt
    68041 ggaacgcatt ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga
    68101 actgcgtaac ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac
    68161 tgacatcatc aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc
    68221 tcagtttgcc gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg
    68281 cgctgtcacc aacgtcacca actttggcgc gtttgtcgat attggcgtgc atcaggacgg
    68341 cctggttcac atctcttcat tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa
    68401 agcgggcgac attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc
    68461 cctgaccatg cgtctggacg agcagcctgg cgaaaccaac gcccgtcgcg gcggcggtaa
    68521 tgaacgcccg caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc
    68581 cggtaatagc gcgatgatgg atgcgctggc ggcggcaatg ggcaaaaaac gttaaacgcc
    68641 ctgattgacc cctaaacctg cgcgataagg tgtccacgcc gcatctggca acgaacgcct
    68701 tgtctgacat aaaagtgccg gagaatatct ccggcctttt tattccacaa ccaaactcat
    68761 aatatattcc ggcaatattt atcacttcat taacaactga aaccttaatt aaacattagc
    68821 cagtccgggt aattcactat tcgaattata ttttcgctgc gatataacct tgagccacat
    68881 caacattgag tcagattatt attcaaacca acattcgcac acattttaag tattgctgat
    68941 agaaaccatt ctcattatca ttgtgttgtt gattatttaa tctctccttt gttggcaaat
    69001 catctggtct catgtcgctg tcaaacgccc catgaggtag ttatccagtt aatgagaaac
    69061 aagtaggcac ctatgcaata cactccagat actgcgtgga aaatcactgg cttttcccgt
    69121 gaaatcagcc cggcatatcg ccaaaaactg ctttctcttg gcatgttacc tggctcctct
    69181 tttaatgtgg tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg
    69241 agcctggtat tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa
    69301 tacggtgata acaacaatga aaaaattaac cataggctta attggtaatc caaattctgg
    69361 caagacaacc ttatttaacc agctcactgg cgcacgtcag cgtgtaggta actgggctgg
    69421 cgttaccgtc gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt
    69481 ggacctgccc ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca
    69541 aatcgcctgt cactacattt tgagtggcga cgccgacctg ctgattaacg tggtggatgc
    69601 gtctaacctt gagcgtaacc tgtacctgac gctacaactg ctggaactcg gcattccctg
    69661 cattgtggca ctgaacatgc tcgacattgc cgagatgcaa aatattcgta ttgaaattga
    69721 tgctctgtcg gcgcgtcttg gctgcccagt gatcccgctg gtttcaaccc gtggtcgcgg
    69781 tattgaagcg ctcaagctgg cgattgatcg ctataaagct aacgagaatg tggaactggt
    69841 gcattacgca cagccgctac tcaacgaagc agattcactg gcaaaagtga tgccttccga
    69901 catcccgctg aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag
    69961 ccgcgcctac gccggtgaag cgtcgcagca tctggatgcc gccctcgccc gtctgcgtaa
    70021 tgagatggac gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat
    70081 ctgtgatgtg gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga
    70141 taaaatcgtg ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat
    70201 gttcctgctg gctatcaaca tcggcggggc gttacagccg ctgtttgacg tcggctccgt
    70261 ggcgctattt gtgcatggta ttcaatggat tggctacacg ctccacttcc cggactggct
    70321 gactatcttc ctcgcccagg gcctgggtgg cggcattaac accgtgctgc cactggtgcc
    70381 gcagattggc atgatgtacc tgttcctctc cttccttgag gactccgggt atatggcgcg
    70441 tgcggcgttt gtgatggacc gtctgatgca ggcgctgggc ttgccgggga aatcctttgt
    70501 gccgttgatc gtcggtttcg gttgtaacgt accgtcggta atgggtgcac gtacgcttga
    70561 tgcaccgcgt gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg
    70621 tctggctatc ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg cgctggcggt
    70681 cttctcgctg tatatgctgg gtattgtgat ggcggtgctg actggcctga tgctcaagta
    70741 caccatcatg cgcggtgaag cgacgccgtt tgtcatggag ttgccggtct atcatgtacc
    70801 acacgttaaa agcctgatta tccagacctg gcagcgtctg aaaggcttcg ttctgcgtgc
    70861 tggtaaagtg atcatcatcg tcagcatttt cctgagcgct ttcaacagct tctcgctgag
    70921 cgggaaaatc gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgatcac
    70981 cccggtcttc aagccgattg gcgtacatga agataactgg caggcaacgg ttggcctgtt
    71041 tacgggcgca atggcgaaag aagtggtggt gggtacgctc aacaccctct acaccgcaga
    71101 aaatattcag gacgaagagt tcaatccggc ggaattcaac ctcggtgaag agctgttcag
    71161 tgcggtagat gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa
    71221 ccccattgaa gctagcaaag gcgacggcga aatgggtacc ggggcgatgg gcgtgatgga
    71281 tcagaaattc ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtatgt
    71341 accgtgtatc tcggtgatgg gggctatcgc ccgtgaatca agccgtggct ggatgggctt
    71401 ctccatcctg tgggggctga atatcgctta ctcactggca acattgttct atcaggtcgc
    71461 cagctacagt cagcatccaa cttacagcct ggtgtgcatt ctggcggtta tcctgtttaa
    71521 catcgtggtt atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc
    71581 aacccgcaag tcggtaagca gttgctgcgc agccagcacc accggtgatt gccattaatg
    71641 gcttcactta ttcaggtgcg cgatttgctg gcgttacggg gccgtatgga agcggcccag
    71701 ataagccaga cattgaacac tccacagcca atgattaacg ccatgctgca acaactggaa
    71761 agtatgggca aagccgtgcg gattcaggaa gaacctgacg gctgcctctc tggcagttgt
    71821 aaaagctgcc cggaaggaaa agcctgtctg cgcgagtggt gggcgctgcg ttaaccttac
    71881 tccatcgcct gttttggaaa gcccggtatg cgtctgcatc cgggcttttt tgcgtgcggc
    71941 tttccataaa aatgcaactc ttgcagcacg gcgtaagttc ctttgaaagc atctcgcagg
    72001 gatgaaaact cgctaataca caggagtgaa gtggcgcgta gagtcgcggc attcaaacaa
    72061 caggtgaagg aacgccatga gcaaaaagca gagttccacc ccacacgatg cgctgttcaa
    72121 actcttttta cgccaaccgg acacggctcg tgattttctt gcgtttcatt taccggcacc
    72181 cattcacgcg ctttgtgata tgaaaaccct caagctggag tcgagcagct ttattgatga
    72241 cgatctgcgt gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg
    72301 atacatctat tgtctgattg aacatcaaag cacctcaaac aaactgatcg catttcgcat
    72361 gatgcgttac gctattgccg caatgcaaaa tcaccttgat gccggataca aaacgttgcc
    72421 gatggtggtg ccattgttgt tttaccacgg tattgaaagc ccctatccct attcgctgtg
    72481 ttggctggat tgtttcgccg atcccaaact ggcaaggcag ctttatgcct ccgcatttcc
    72541 gctgattgat gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac gtatggcgct
    72601 gctggagtta attcaaaaac atattcgtca acgcgacctg atggggcagg tagagcaaat
    72661 ggcctgctta ttaagtagtg gatacgctaa tgacagacaa atcaaagggc tgtttaatta
    72721 catactgcaa accggcgacg cggtacgttt taacgatttt atcgacggcg ttgccgaacg
    72781 ttcaccgaaa cacaaggaga gtttaatgac aattgcggaa agattgcggc aggaggggga
    72841 acaatccaaa gccctgcata tagccaaaat aatgcttgaa tccggagtcc ctcttacaga
    72901 catcatgcgc tttaccgggg tgtcagaaga agagttggct gcggcgagcc agtaaatttc
    72961 tgtctcgcca tttcaaaagt tgcctacacc ctctgcttca acgccaccag caggtgacaa
    73021 aactcgaccg gatgcgaaat aaacggcgca tgggccgctt tggcgaagat atatgattcg
    73081 ctgtgaggcc aaagtttatc cagcatcggc accactttgc gcggcaccag accgtcgaga
    73141 tagccataca atcgcaaaaa cggcatgggc acgttttgca gcggctgacg gagatcgacc
    73201 gttttcagga tttccagccc gccattaagc acgtcaacct ccggcatcgg taacgccaga
    73261 acggttttct tcaacgcccg cgcatcctgg cgcgccgttt cagtccccat ggtttgtaac
    73321 gccaggaacc gctccactgt acgctgaaaa tcatcactga gttgctgctg aaatcccgcc
    73381 agcacgtccg gttttatccc cggccactcg tcacgagcac taaaacaagg tgacgacgcc
    73441 acggtgacca gcgcctgaac acgctcggga tgggttaacg caatctggct tgccaccagc
    73501 ccgcccagac tccagcctaa ccaaatggct ttatcaggtg cctgttgcag cacggcttcg
    73561 gccatatcag caagtgacag cgcaccaaat ccccggctac gcccgaagcc gggcaggtca
    73621 acaaggtgca gcgtaaaatg cgagctaagt tcctcgtcaa tgcaacgcca cacttcggca
    73681 ttcagtcccc atccgtgcag cagcacaaga tgaacattcc cctgaccttt ggtctgccac
    73741 cagatgttat tcatccgcta ttgttctctt ttgacttaca aggatgaaca tatgctaaca
    73801 gtaccgggat tatgctggct atgccgaatg ccactggcgt taggtcattg ggggatttgt
    73861 tcggtctgct cacgcgctgc ccgtacagat aaaacgttat gcccacaatg tggattaccc
    73921 gccacacact cacatcttcc ctgcggtcgc tgcctgcaaa aaccaccgcc ctggcaaaga
    73981 ctggtcacgg ttgccgacta tgcgccgccg ttaagtccgc ttatccacca gcttaaattt
    74041 tcccggcgca gtgaaatcgc cagcgccctg tcacgtctgt tactattgga agtcttacac
    74101 gctcgtcgca ccaccgggtt gcaattgccg gatcgcatca tcagcgttcc gttatggcag
    74161 cggcgtcact ggcgtcgggg atttaatcag agcgatttgc tgtgtcagcc gttatcacgc
    74221 tggttgcact gccgatggga tagcaaagcc gtcacacgaa cacgggccac tgcgacccag
    74281 cattttctca gtgcccggct acgcaagcgc aacctgaaaa atgcctttcg tcttgaattg
    74341 cccgtgcaag gtcgccatat ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc
    74401 gcagagattg cgcagttgct tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt
    74461 tgtcgaacct tgtagagcct cgatgatggg cgtattataa ccaactaaaa tagtcaacta
    74521 ttaggccatt actatgatcc gtatttccga tgctgcacaa gcgcactttg ccaaactgct
    74581 ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg attaaccctg gcacgcctaa
    74641 cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg gaagccaccg acactgccct
    74701 gaaatttgac ctgctgaccg cgtatgttga tgagttaagc gcaccatacc tggaagatgc
    74761 agagatcgac tttgttaccg accagttggg ttctcagtta acgctgaaag ccccgaacgc
    74821 caaaatgcgt aaagtggcag acgatgcacc gctgatggag cgcgtggagt atatgctgca
    74881 gtcgcagatc aacccacagc ttgctggtca cggtggtcgc gtttcgctga tggaaatcac
    74941 cgaagacggt tacgccattc tgcaatttgg cggcggctgt aacggttgtt ccatggtcga
    75001 tgtgacgctg aaagaaggga tcgagaagca gctgctgaac gaattcccgg agctgaaagg
    75061 tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc tactactaag ttatcccctc
    75121 ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc
    75181 cagacgttgg cacagcattg ctttggcaaa tccctccctt cgtttccccg cgataatatg
    75241 accaacctct cataatttaa atttaccccg ctctggtgat tctcaaacgc cagatgttac
    75301 ccgtatcatt cacatgggta ccaaacatac tcctgacatc tgactacaat aattagtttt
    75361 agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg attgggataa ttagagtttg
    75421 tcgtcagaaa attgacgtta cccataacaa atgaaaggcc aggtaaatca tgccattagt
    75481 cattgttgct atcggtgtaa tcttgttgtt gctcctgatg atccgcttca aaatgaacgg
    75541 cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta atgcaaggaa tgccgctgga
    75601 taaagttatt ggctccatca aagccggtgt cggcgggacg ctcggtagcc ttgccctgat
    75661 catgggtttt ggcgcaatgc tgggcaaaat gctggcagac tgcggtggcg cacaacgtat
    75721 cgccaccacg ctgattgcca aatttggtaa aaagcacatc cagtgggcgg tggtgttaac
    75781 cggttttacc gttggttttg ccctgttcta tgaagtgggc ttcgtgctga tgctgcctct
    75841 ggtgtttacc atcgcggctt ccgcgaatat tccactgctt tatgttggtg taccaatggc
    75901 ggctgcactg tctgtgaccc acggcttcct gccaccgcat ccgggcccga ctgcgattgc
    75961 caccattttc aatgccgata tgggtaaaac cctgctgtac ggtactattc tggcaatccc
    76021 gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg aaaggtatcg ataagccaat
    76081 cccggaaggt ctctacagcg cgaaaacctt cagcgaagaa gagatgccga gctttggcgt
    76141 cagcgtctgg acctctctgg tgccggtagt actgatggcg atgcgtgcga ttgccgaaat
    76201 gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag ttcctcggtg acccggtaat
    76261 ggctacgctg attgccgtgc tgattgccat gttcaccttt ggtctgaacc gtggtcgttc
    76321 aatggatcag attaacgaca cgctggtttc ttccatcaaa atcattgcga tgatgctgtt
    76381 gatcatcggt ggtggcggtg cgttcaagca ggtgctggta gacagcggcg tggacaaata
    76441 cattgcttcc atgatgcacg aaaccaacat ttctccgctg ctgatggcct ggtcgattgc
    76501 tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg gcaatcactg cgggtggtat
    76561 cgcggcaccg ctgattgcaa cgacgggtgt tagcccggag ctgatggtta ttgcggtggg
    76621 ttccggtagt gtgattttct ctcatgtgaa cgatccgggc ttctggctgt tcaaagagta
    76681 ctttaacctg actatcggcg agaccatcaa atcctggtcg atgctggaaa ccattatctc
    76741 ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg atttgataac actgactgcc
    76801 ggatgcatcg catccggcat caaccgcact ctacttcttc ttcgctgcag ctctgcgccg
    76861 tctgtccaaa tccttcagca acttgttcac gccatcatcg gcaaacatcg actcaagcgt
    76921 tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg gtgccaggaa tattcaccgg
    76981 ttcggccata tccagccagt cttccggctg tagtcctaac agagcactgt tactgtcggc
    77041 aatgtagcgc tgcaaaccac ggttcagcgt cggcgtcatc gacatcaacg atgccttatg
    77101 cccggcacgt tttggcagac aaccatattt atgcagtgca tccagcagcc cttgcttcgc
    77161 cagttcgcga tcctgataca gaccgcgcag taccacttca tccggataca gccccagggt
    77221 tttgcccagc gttagatccc cgctctccca gtaaccgcgt agcgtaggca ggtcatgtgt
    77281 cgccgcaacc gccatcgact gctccggata cgctttcggt gcacggaacg tcttctcgtg
    77341 gtcgttttcg aaatagagca ctttgtagga gtacacaccg ctgctgcgca gcttaccgac
    77401 aatctctacc ggtacggtac cgagatcttc accaatcacc atacagcgat gacgtttact
    77461 ttcgagtgcc agaatcgaga gcagatcatc caccggatag tgaacatacg cgccctgatc
    77521 tgccgtctcg ccatacggta tccaccacaa acgcagcatc gacatcacat ggtcaattcg
    77581 taatgcgccg cagttttgca tattggcacg caacagctcg ataaacggtt cataggcacg
    77641 cgcggtgatg atatgcgggt ccattggcgg taatccccag ttctgcccca acgggccgag
    77701 gatatccggc ggcgcgccaa ccgacgcttt caggcaatat agttcacggt cacaccaggt
    77761 ttccgcccca ccttccgcta cgccaaccgc cagatcacga tacaagccaa tcggcatttc
    77821 atagccctgg cttatctccc agcaggcggc aaactggctg taagccagcc actgcaacca
    77881 gagataaaaa tcgacgtcat cacgatgttc ttcgcagaac tgacgcactt ctggtgaatc
    77941 cacgttctga tacatctctg gccatgcagg ccagccccag cgcatttcgt cctctttcac
    78001 ttgctgggca tgtagcgcat caaaggctgc ctgccagaac aggctgtcgc cctgctctgc
    78061 aacaaactgg cgaaacgcgg tcatctgctc atgatcacgt tgcgcgaaac ctttccacgc
    78121 cattcgtaat gctgtcattt ttagggcggt aaccgtggag taatcgaccc agtcggcatc
    78181 gcgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc caccaggcct gagcctcttc
    78241 gctaagatgg aaatcttcaa cggcgttaac gtcgatataa atcacattca gccaacggcg
    78301 agaagacggg ctgtatgggc tggcactctc cggatttgcc ggatagagcg catgaatcgg
    78361 gttcaggccg atgaacgacc cgccacgttt tgccacatcc accagcatcg ctttgagatc
    78421 gccaaaatcc ccaatacccc agtttttttc cgatcgcagc gtataaagct gaacgcaggc
    78481 accccacagc ttttgtttat tcagcaacgc ctgcggttcg taacagcgtt tcggggcgac
    78541 aatcacccgg caatgcgcgc gctggtcgtc ctgggtgagt gtcagcgtgt gataaccttc
    78601 cggcagcttc gtcggtagat tgaacgcttt gccccccgtt acatggcctt tgtactgtgt
    78661 tccttcttcg gtggtcagca gccagctata ttcgccgctg ccctccacca ccatcggcat
    78721 ttttttgccg ctggtataaa ccatgacatt cgggactggc gttaccgcca ctttcgtggc
    78781 ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt tcggcgctaa tcgactgcgg
    78841 tttaccgtag gcattgatgt aattggggct aatccccgcc gccagcgcgg cattatccag
    78901 acgtttgctt tccatcgagc ttccttagcg ttttgcctgc cagatacgag cctgataatc
    78961 gcgaatagag cgatccgagc taaacatacc gcagcgggcg gtattgagga tcgccgcgcg
    79021 agtccaggcc tcctggtcgc ggtacagcac atccacctgc ttttgtgcct caacataggc
    79081 tgcgaaatcc gccatcacca gatacggatc gccgccttgt ttgccgatac tgtgcagcat
    79141 ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg ctttccagct ctttcaatac
    79201 tgcgtccagc accttatctt tcttccgcca tttcaccggg tcgtagcctt tggccagaat
    79261 agccttcact tgttccacgg tatgaccaaa aataaagata ttttcttcac cgactttctc
    79321 ggcgatttca acgttcgcac catccagcgt accgacagta agcgcaccat tgagcgccag
    79381 tttcatattg ccggtaccgg aagcttcttt acctgcagtc gaaatttgtt cggagatatc
    79441 cgccgccggg atcagttttt ccgccgccga aacgcaataa tccggcagga acaccacctt
    79501 caacttatcg ccaaccagtg gatcgttgtt gatcacgtca gccactttgt tgatcgcaaa
    79561 gataatattc ttcgccaggt agtagcccgg tgccgctttc gcgccgaaga ggaagacgcg
    79621 cggtacgcga tcagcctgcg ggttttcacg aatttctttg tacaacgcca gaatatgcag
    79681 cagattcagg tgctggcgtt tgtactcatg cagacgtttg atctgaatat cgaaaatcgc
    79741 ctgtggattg atctcaatac cagtacgaac tttcacaaac tccgccagac ggactttatt
    79801 cgcctgcttg atctcgcgat attgctgacg gaatttcgca tcatcagcga atttttccag
    79861 attgatcagc tgatcgagat cgttagccca ctctttttgc agtgatttat ccaacagagc
    79921 cgccagtgca gggttgcact gtttgatcca gcgacgtggg gtaataccgt tggtgacgtt
    79981 atggaatttg ttcggccata gctggtgata ttccgggaac agatctttca ccaccagatc
    80041 cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca accacacaca ggttcgccat
    80101 atgcacttgt ttatcgtgca ccaccgccag tttggcccac actttttcat cgcccggcca
    80161 ggttttctcc accagcgttt taaagcgagt attaatttcg ttaataatct gcatatggcg
    80221 cggcagtaag cctttcacca gtttcacatc ccagcgttcc agcgcttctg gcatcagggt
    80281 atggttggtg taagcgaaag ttttgctggt aatggcccag gcgtcatccc agctcatctg
    80341 gtgctcatcg atcagcacgc gcagcagttc tggaatcgcg atagttgggt gggtatcgtt
    80401 cagctgaata acttcgtaat ccgccagttc gtgcagttta cgccccgcca gatgatggcg
    80461 acgcaaaata tccgctaccg aacaggcaca ctggaagtac tgctgcatca agcgcagctt
    80521 tttaccggca gtatggttgt cgtttggata gagaactttg gtcagttttt ccgcattgat
    80581 gccctgctgt tcggcacgca ggaaatcacc gtcgttaaat ttagtcagat caaacggatg
    80641 cgcgtgcgtc gcctgccaca gacgcagcgg ctgcgccacg ccattacgat agccgacaac
    80701 ggggagatcc cacgcttgac cggtaatggt aaactccggc tcccagcgtc cgtctttcgt
    80761 cactttaccg ccaatcccta cctgcacatc cagtgcttcg ttgtggcgga accacgggta
    80821 gttactgcgg tgccagtcat ccggcgcttc aacctgtttg ccatcgacaa aagactggcg
    80881 gaacaaacca tattgatagt tcagaccgta acccgtcgca gactgaccga cagttgccat
    80941 tgagtcgagg aagcacgccg ccagacgtcc cagaccaccg ttacccagcg ccgggtcgat
    81001 ctcttcttcc agcaggtccg tcagattgat gtcataagcc ttcaacgaat cctgtacatc
    81061 ctgataccag ccgagattca acaggttgtt gcccgtcagg cgaccaatca aaaactccat
    81121 tgagatgtag ttaacatgtc gctgattcgc caccggcttg gcgaatggct gagcacgcag
    81181 catttcggcc agtgcttcac tcactgctag ccaccactgg cgaggagtca tttcagccgc
    81241 agaatttaag ccataacgct gccactgacg tgaaagcgct tcctgaaatt gcttatcgtt
    81301 aaaaataggt tgtgacatag gagttccact tttcttagat tttcaacaca acgttatcgc
    81361 tagtttgcca ggctcgatgt tgaccttcct catcctgcgg gggattaggc agggaggagt
    81421 tgcggggatg agcaaggaaa tgtgatctca accacttaaa gctagtgcaa accacaggat
    81481 tagcagcaaa tcaatgcaat acagcgcaga aaatctgtat ctaagtgcaa aaaatggccg
    81541 ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta agtaaaggag tgaaacagtt
    81601 tcataagtaa aatatccagt gtgctccatc tcattcttaa tagatttatt aagatcatct
    81661 ttttagatgg cactttcatc aggaatgaag aagaaaccct tgcttaaatg aatctgatga
    81721 acataaggga aaccagtatt cacgctggat cagcgtcgtt ttaggtgagt tgttaataaa
    81781 gatttggaat tgtgacacag tgcaaattca gacacataaa aaaacgtcat cgcttgcatt
    81841 agaaaggttt ctggccgacc ttataaccat taattacgaa gcgcaaaaaa aataatattt
    81901 cctcattttc cacagtgaag tgattaacta tgctgattcc gtcaaaacta agtcgtccgg
    81961 ttcgactcga ccataccgtg gttcgtgagc gcctgctggc taaactttcc ggcgcgaaca
    82021 acttccggct ggcgctgatc acgagtcctg cgggctacgg aaagaccacc ctcatttccc
    82081 agtgggcggc aggcaaaaac gatatcggct ggtactcgct ggatgaaggt gataaccagc
    82141 aagagcgttt cgccagctat ctcattgccg ccgtgcagca ggcaaccaac ggtcactgtg
    82201 cgatatgtga gacgatggcg caaaaacggc aatatgccag cctgacgtca ctcttcgccc
    82261 agcttttcat tgagctggcg gaatggcata gcccacttta tctggtcatc gatgactatc
    82321 atctgatcac taatccagtg atccacgagt caatgcgctt ctttattcgc catcaaccag
    82381 aaaatctcac cctggtggtg ttgtcacgca accttccgca actgggcatt gccaatctgc
    82441 gtgttcgtga tcaactgctg gaaattggca gtcagcaact ggcatttacc catcaggaag
    82501 cgaagcagtt ttttgattgc cgtctgtcat cgccgattga agctgcagaa agcagtcgga
    82561 tttgcgatga cgtttccggt tgggcgacgg cactacagct aatcgccctc tccgcccggc
    82621 agaataccca ctcagcccat aagtcggcac gccgcctggc gggaatcaat gccagccatc
    82681 tttcggatta tctggtcgat gaggttttgg ataacgtcga tctcgcaacg cgccattttc
    82741 tgttgaaaag cgccattttg cgctcaatga acgatgccct catcacccgt gtgaccggcg
    82801 aagaaaacgg gcaaatgcgt ctcgaagaga ttgagcgtca ggggctgttt ttacagcgga
    82861 tggatgatac cggcgagtgg ttctgctatc acccgctgtt tggcaacttc ctgcgccagc
    82921 gctgccagtg ggaactggcg gcggagctgc cggaaatcca ccgtgccgcc gcagaaagct
    82981 ggatggccca gggatttccc agcgaagcga ttcatcatgc gctggcggca ggcgatgcgc
    83041 tgatgctgcg cgatattctg cttaatcacg cctggagttt gttcaaccat agcgaactgt
    83101 cgctgctgga agagtcgctt aaggccctgc cgtgggacag tttgctggaa aatccgcagt
    83161 tagtgttatt gcaggcgtgg ctaatgcaaa gccaacatcg ctatggcgaa gttaacaccc
    83221 tgctagcccg tgctgaacat gaaatcaagg acatcagaga aggcaccatg cacgcagaat
    83281 ttaacgctct gcgcgcccag gtggcgatta acgatggtaa tccggatgaa gcggaacggc
    83341 tggcaaaact ggcactggaa gagctgccgc cgggctggtt ctatagccgc attgtggcaa
    83401 cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt gacccgctca ctggcgctaa
    83461 tgcagcaaac cgaacagatg gcacgccagc acgacgtctg gcactacgcc ttatggagtt
    83521 taatccagca aagtgaaatt ctgtttgccc aagggttcct gcaaaccgcg tgggaaacac
    83581 aggaaaaagc gttccagctg atcaacgagc agcatctgga acagctgccg atgcatgagt
    83641 ttctggtgcg catccgagca caactgttat gggcctgggc acggctggat gaagccgaag
    83701 cgtcggcgcg tagcgggatt gaagtcttgt cgtcttatca gccacagcaa cagcttcagt
    83761 gcctggcaat gttgattcaa tgctcgctgg cccgtggtga tttagataac gcccgtagcc
    83821 agctgaaccg tctggaaaac ctgctgggga atggcaaata tcacagcgac tggatctcta
    83881 acgccaacaa agtccgggtg atttactggc aaatgaccgg cgataaagcc gccgctgcca
    83941 actggttgcg tcatacggct aaaccggagt ttgcgaacaa ccacttcctg caaggtcaat
    84001 ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt tgaacctgcg gaaattgttc
    84061 tcgaagaact caatgaaaat gcccgcagtc tgcggttgat gagcgatctc aaccgtaacc
    84121 tgttgctgct taatcaactg tactggcagg ccggacgtaa aagtgacgcc cagcgcgtgt
    84181 tgctggacgc attaaaactg gcgaatcgca ccggatttat cagccatttt gtcatcgaag
    84241 gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact caatacgctg ccggaactgg
    84301 aacagcatcg cgcacagcgt attctgcgag aaatcaatca acatcatcgg cataaattcg
    84361 cccatttcga tgagaatttc gttgaacgtc tgctaaatca tccggaagtg ccagaactga
    84421 tccgcaccag cccgttgacc caacgtgaat ggcaggtcct ggggctgatt tactccggtt
    84481 acagcaatga acaaattgcg ggcgaactgg aagtggcggc aaccaccatc aagacgcata
    84541 tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca ggctgccgtg cagcacgcgc
    84601 agaaactgct gaagatgatg ggttatgggg tgtgagttta gccggataat gcattggatc
    84661 cggtttacat ctttacatca ttcaatactc acccgcgtta cgccatctgt ttctatcaaa
    84721 ccaaaccgca ccggcaagaa acgctccacc accgcgatat tggtcagcag atggcatgaa
    84781 ggatgggcga ccttaaattc tcccgcgccc gccagcgcca tcggcagtac cagttggtcg
    84841 gcgagatatt cccccaccgc cgctgggctt gccaggtagc gtttcacctc tttcactaac
    84901 tgcgcggcta ccacctcggc actgacgcgc ttttcaccga cgacgaaaaa gcgttcagtg
    84961 atattttcac tttcgacttc cagcgagacg gtattacccg gcccctggtc gcgcggcagg
    85021 ttatgaatat tctgttcatg cagggaaaaa ctccccgcca gtgtagcgat ttcacgctca
    85081 gcaacatggc gcggcacacc agctaataga acctctccac gcatctgcac aatgttcccg
    85141 cgctcgccaa gttgcaaggt gttaaacgat gccacgggcg agacttccgt tgccaccacg
    85201 ccgcctccgg caggataaaa accgtgacgt aacagcgtgg tttgctgatg aattcctatt
    85261 ttcgccagca gcggctccag cacccggcgg ataaaatccg caggcggggc cgacgggtta
    85321 tcggtgcctc cgctcacttc aacacgcgaa ggtccatcgg caaaccacag cgcgggcagc
    85381 accgtttgca gcaccagcgt acagcttccg gcactaccga tagcaaagcg gtactcgccg
    85441 ccgcgcacga tgccaggccg gaagaccaga cgctgcgacc ccagctccgc gccttccacc
    85501 gttgccctac aaatttccgc agccgctttt accgcggtca gatgctggcg caacagcccc
    85561 ggtttcgccc gcccggcacg aatgctggtg atggtaaatg gctggccggt tatcatcgac
    85621 aggctcagcg ccgagcgcag gatctgcccg ccaccttcgc cctgtgcgcc atccagcgca
    85681 atcatccttt tcatcattta tccttttacg cacaccacct gacgcagggt atagataact
    85741 tccaccagat cgctttgtgc cgccatcacc gcatcaatat ctttatacgc catcgggatt
    85801 tcgtcgatca cttcggcatc tttacggcat tccacatgcg cggtggcacg aatttgatct
    85861 tccacgctga acagtttttt cgctttagta cggctcatta cccgcccggc accgtggctg
    85921 cacgaacaga aagattcttc atttcccagc ccacggacga taaagctttt tgctcccatc
    85981 gaccctggaa taattccata ttgaccagca cgcgcagaca ccgcgccttt acgcgtcacg
    86041 tagatctctt caccaaagtg ctgttctttt tgcacatagt tgtggtgaca gttgatctct
    86101 tccatcgcca gcgtttgtgg ctgtctgacc gttttctgcg taatgctctg caatgccgtt
    86161 accacgtttt ccatcatcgc atcgcggtta aggctggcaa aaagctgcgc ccaggccacg
    86221 gctttcaggt aatcatcaaa gtattccgta ccttccataa agtacgccag atcacgcgac
    86281 ggcaacgtct caagcgtttc ctgcatctct ttttgtgcca gatcgataaa gtaagtcccg
    86341 atggcgttac caattccgcg tgaaccggag tgcagcataa tccacacctg gtccgactca
    86401 tcaaggcaga tttcaataaa gtggttaccg gttcccagcg ttcccaggtg tttatagtta
    86461 ttggtattca ggaaacgggg atatttttgc gttaaccact gataaccggc ttcaagctca
    86521 gcccatttcg catcgacgtt aacaggcggg ttttcccatg cgcctttatc acgtttacaa
    86581 cggccagtgg tacgcccgtg cggcacggcc atttcaatcg cctgacgcag ctctgccagg
    86641 ttttcaggca ggtcttccgc cgttaacgcg gtacgcagcg cgttcattcc acagccaata
    86701 tccacgccca ccgccgccgg aataatcgcc cctttggtcg ggatcacgct accaatggtg
    86761 gaacctttac ccaggtgtac atcaggcatt accgcaatat gtttgaaaat aaacggcatc
    86821 ttcgccgtat taataagttg ctgacgcgca tcggcttcta ccggcacgcc tttggtccac
    86881 atttttaccg gggcattttc agtggtcagt aattcgtaat tcattttgtt tttctctttt
    86941 cgttgtttgc tgtcgtgata attgcaaccg tcgtgccaga aaattaaata agcagattaa
    87001 ttttttaatt cattgttttt aaataggtta aatctttacg tccgtaaacg gagattttcc
    87061 acaaaaccaa ttcaccgata atgaaatatc gtcttttata aggatatcta agatgcgtaa
    87121 aacagtggct tttggctttg tcggtaccgt gctggattat gccggacgcg gcagtcagcg
    87181 ctggtcgaaa tggcgtccga cgctttgttt atgccagcaa gaatcgttag tcatcgatcg
    87241 gctggaattg ttgcacgaca cccgctcgcg ctcgctattc gaaacgctta aacgcgatat
    87301 cgccagcgtt tcgccagaaa cagaagtcgt gggcgttgag attgaactgc ataacccgtg
    87361 ggatttcgaa gaggtttacg cctgcctgca tgatttcgcc cgtggttacg agtttcagcc
    87421 agaaaaagaa gactatttaa ttcacatcac caccggcacc cacgtcgcgc agatttgctg
    87481 gtttctgctg gcggaagcgc gttacctgcc cgcccggctg atacaatctt cacctccgcg
    87541 caaaaaagaa cagccgcgcg gcgcaggtga agtgacgatt atcgatctcg atttaagccg
    87601 ttataacgcc atcgccagcc gctttgccga ggaacgccag caaacgcttg attttcttaa
    87661 gtccggcatt gccacgcgta acccccactt caaccgcatg attgagcaga tcgaaaaagt
    87721 ggcgatcaaa tcccgcgcgc cgattctgct taacggtcca accggcgcgg gcaagtcatt
    87781 tctggcgcga cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg gcgcgtttgt
    87841 ggaagtgaac tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt ttggtcatgt
    87901 aaaaggcgcg tttaccgggg cgcgggaatc tcgtgaaggt ttattacgca gcgccaacgg
    87961 cggaatgttg tttcttgatg agattggcga actgggcgca gacgaacagg caatgctgct
    88021 aaaagccatt gaagagaaaa ccttttaccc gtttggcagc gatcgccagg tgagcagtgt
    88081 ttttcagctt atctccggaa cggtgcgcga tttgcgccag ctggttgccg aaggcaaatt
    88141 tcgcgaagat ctgtacgcgc ggatcaatct ctggaccttc accctgccgg gtctacgcca
    88201 gcgccaggaa gatattgaac cgaaccttga ttatgaagtg gagcgccacg ccacactcac
    88261 tggcgacagc gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt ttgcgacctc
    88321 tccccaggca acatggcgcg gtaactttcg cgaactttct gccagcgtca cgcggatggc
    88381 cacctttgcc actagcggac gcatcactct ggacgtggtt gaagatgaga taaaccgtct
    88441 gcgctataac tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg gcgcagaggc
    88501 agaaaacatc gacctttttg acaggctgca actggaacac gtgatagctc tctgtcgcca
    88561 ggcaaagtcg ctttccgctg ccggacgcct gctttttgat gtttcgcgcc agggcaaagc
    88621 cagcgtcaat gacgcggatc ggctacgcaa atacctggcg cgttttggtc tgacgtggga
    88681 agccgtgcag gatcagcaca gctccagttg aatatgatgg tccgtcagca cctgcattac
    88741 gcttactggc ggcggggcgt cggtgtagac ggcatctacc atgctgatgc tgcccatatt
    88801 gaccatcgcg ttacggccaa atttcgagtg atcgacaacc agcataacgt ggcgcgagtt
    88861 ctcaataatg gcgcgcttgg tgcgaacttc gtggtaatcg aactccagca gcgagccgtc
    88921 gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg agataaaatc
    88981 gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt aattcgccac cggcgagaat
    89041 aatgcggaaa tcttctttta ccatcaacgt gttagcaaca ttgagattgt tggtgacaat
    89101 acgcaaattg ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg tgccgatatc
    89161 gataaacagt gtcgagccgt tggggatttg ctccgccact ttgcgggcaa tcctctcttt
    89221 ttcttcggtc tgggtggctt tgcgatcgtg ccacggcgtg ttaaccgaac tggaaggcag
    89281 cgccgcaccg ccatggtgac gcaggatcag gttttgctcc gccagctcat tgaggtcgcg
    89341 gcgaatagtc tgcgggctga cggagaaatg ctctaccagc tcttcagtac tgacataacc
    89401 ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca tttataaatc
    89461 cctggaatta ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc gctaaaccca
    89521 cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa tcaaaccatc
    89581 cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa atgccacttt
    89641 gcggatcgcg ttcaccacgt agccagacgt agcccatcag cgcatacacc acgccagaaa
    89701 gcccgccaaa ccacggcccg ctgaattttt gctgcacata gccgcttaac agggcgctaa
    89761 tgagagtaat aacaattagc ttaccgctac cgaggcgttt ttccaccgca ccgccgagat
    89821 accaccacca gagcaggtta aagaggatat gcatcagcga gaagtgcatt aacgcgtggg
    89881 tgaagtaacg ccagaactca aatttcagtg ttggatcgaa tggccaggcc agccataaca
    89941 tcacttcctg atcgccgaga atttgcatgg cgataaacac caccacgcag gcgatcatca
    90001 ccacccaggt taccggacct gcgcgttcac gcaaggcggc aaagaaagga taacggcgat
    90061 aatgcaggcc actgccggta tggcctgcct gccagctggc cgccagataa cgcggatctg
    90121 ccgggttttc gagaaaacgc gccagctccg cccgtacgcg ctcggcctgg gactcatccg
    90181 ccagccagac atcgctttgg ttatgttgtt gaatcgtgag gataacaccc tgcgtcgcca
    90241 tgtaatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc atcaacatcg
    90301 ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc cgtacgccac
    90361 ctctgcggga aactgacgtt gccaggcttc aaagccgccg tcaatgctat agaccacatc
    90421 gtagccctgt tgcagcagat actgcgccgc gcctttgctg ctattgccgt gataacacat
    90481 caccatcacc ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg tgtcgttggt
    90541 taaatggaaa gcctgcaccg catgccccat agcgaaactc tgtggatcgc gaatatcgac
    90601 cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa tacattcgaa
    90661 ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccgc acagtttacg
    90721 tcgaagcggc agataaacgc cataatgtta tacatatcac tctaaaatgt tttttcaatg
    90781 ttacctaaag cgcgattctt tgctaatatg ttcgataacg aacatttatg agctttaacg
    90841 aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggtgg cggcatcaat
    90901 ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat gctggaggcg
    90961 caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg tggcctgcgc
    91021 taccttgagc actatgaatt ccgcctggtc agcgaggcgc tggctgaacg tgaagtgctg
    91081 ctgaaaatgg ctccgcatat cgccttcccg atgcgttttc gcctgccaca tcgtccgcat
    91141 ctgcgcccgg cgtggatgat tcggattggt ctgtttatgt acgatcatct gggtaaacgc
    91201 accagcctgc cgggatcaac cggtttgcgt tttggcgcaa actcagtgtt aaaaccggaa
    91261 attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct ggtactcgcc
    91321 aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg cgccacctct
    91381 gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac cggcaaaaaa
    91441 tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa acagttcttc
    91501 gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg cagccatatt
    91561 gtggtgccgc gtgtgcatac ccagaagcag gcctacattc tgcaaaacga agataaacgt
    91621 attgtgttcg tgatcccgtg gatggacgaa ttttccatca tcggcaccac cgacgtagag
    91681 tataaaggcg atccgaaagc ggtgaagatt gaagagagtg aaatcaacta tctgctgaaa
    91741 gtgtataacg cgcactttaa aaaacagctt agccgtgacg atatcgtctg gacctactcc
    91801 ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc aggctattac ccgtgattac
    91861 acccttgata tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt cggcggtaag
    91921 ctgaccacct accgaaaact ggcagaacat gcgctggaaa aactgacgcc gtattatcag
    91981 ggtattggcc cggcgtggac gaaagagagc gtgctgccgg gtggtgccat tgaaggcgac
    92041 cgcgacgatt acgcagctcg tctgcgccgt cgctatccat tcctgactga atcgctggcg
    92101 cgtcattacg cacgcactta cggcagcaac agcgagctgc tgctcggcaa tgcgggggcg
    92161 ataagcgacc tcggggagga tttcggtcat gagttctacg aagcggaact gaaatacctg
    92221 gtggatcacg aatgggtccg ccgcaccgac gatgccctgt ggcgtcgcac aaaacaaggc
    92281 atgtggctga atgcggatca acaatctcgt gtgagtcagt ggctggtgga atatacgcag
    92341 cagaagttgt cgctggcgtc gtaaggatga tgcccggttg aagtgcaacc gggctatttg
    92401 gattccttag actattcatt gtaaataatt gaataacggc ccctgagcta aaatcgccaa
    92461 acttaagcac gttacccaat cgccccactt cttcctctgg tttctatttt ttctgcacca
    92521 atggcaataa tatcccaatg caaaaccaag ctgtgcacta atcgggccga caaaatcagt
    92581 ccaaggctgc ggccaaaaag gggaaaaatt caggggaggc cagacaaacg tcagaaagaa
    92641 atttcggata ttttccagca acgctgattt ctctggagaa atataaagaa tgaaattcga
    92701 gagaattaac cagaaataga ccggcgaaaa gaccagccag caatgcagtc tgttttttaa
    92761 ttcaggaggc gttctgcggg agaattgcca accgatgcca gccattacac tgaaaaaaag
    92821 tgcatattca accattaact caagttgtaa ttcctgaaga gaaatacgca caaccaggag
    92881 cggaaaggag aatattattt ctgccagaaa agttaacgtg aatataagta cggctaagac
    92941 aataaccgtc ggccatacca ataatggcac gataacaaaa ccgagaatta cagggggaat
    93001 ataaagccgg aatttaatca tttttatttt cctggtgatt cggatgatgc gtcatacatc
    93061 gcgcataaac actcatcaat tatccagtcg tctacccaga cctgatgctg tgtgccctgt
    93121 tctggctgct ttggtgggtt ctcaatcgcc tgccgcgcgc gttctttcaa gatggattga
    93181 ttgttgaagg cccaggcatt gatttgggtg gtaatgacgt tatcgatatc gtcctggtta
    93241 tctctgatat aatcctcaca atatctgttc aataaacagg caagagaaac ggtcgaccaa
    93301 aagccgggaa ctgaattaat ggcatccagc aatggcgcaa gagcgatatc ggcacggaaa
    93361 aacatttcca gcatatactg taattcgaca gtttcatcgc cggataccca atcagtcata
    93421 tactggcagg caaatcgttg caaaattttc cattcatcct cacgccaatt ttctttactg
    93481 gcaaggttga tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg cccctgtgca
    93541 acaaattcca gtaaacgcgg cagaaaatag cggacctcat cgctattttg cggatcaggc
    93601 cctgggctac tattccaggc attgattaag gaatacggga tcgctcgtaa cgatgtactc
    93661 cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac aaatttctgc
    93721 ggcacccgat aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg catggcgggg
    93781 ctgggttgca tagttacccc ctcgcccgct gatcgcgtgt aaacggttga tagacaaaac
    93841 gagcattcgc cgcgttcagg cgaataaggg cgctacgcga aagggaaaag ctggggcgaa
    93901 tgcaactatt gcagtctcga cagctttcct ctgcgcaatt ttccactgag taatgacgcg
    93961 tgtagagttt cagttcggcc tgcttatcgc gaaatccgcg taaactgtta ttgttatgaa
    94021 atttccaggc atctttaaag acctgaaaga tatagacatc ctcaatccag gagtcacatt
    94081 ttttaagcgg gcgctttgct attatcaccc gctcatccgg atttagcatc gccgtaaatt
    94141 gtacggtgac gtcatttgtc gtgttcaggt cacattgaac atctggtaca tcactgatat
    94201 aggggtcaaa tccaaaatca gcaccacagg cacaaagacg gctaatggtg gcggtatcga
    94261 gggaaaatcc cggccataca ttgctggacg ttaagccaat atttaatgtg acattatatc
    94321 gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc cccttcatcc agggcctggt
    94381 taatgagttg ctgaacaatg ccttcatccc agtatgccga tttgcagttg gcaatcgtaa
    94441 tatccacgcc tgtgaaggga tcagtaccgg caaggcttgc aaataatgta ttaactaatt
    94501 ccatttttat tatccagctt taaggtagcg tcttatacat cgggacataa cctacatctt
    94561 ccaccaaact ctgtccctgc ggcgttaaaa accactccaa caatttttgt gtctctgacg
    94621 tcgtattttc ccgcgttacc ataaaggcat cgacgatata gggatatttg ccgttgcgaa
    94681 tgttttcggc agtcggtgca ataccgttaa tcgctagcag ttttatattt ttatcgtcgt
    94741 tcatttgtgt tgcgtaataa cggaaggtgt aacctattgc gttgtttgta ttacggtatt
    94801 cggcaacgac tttaatcatt ccctccatca tacttgccac ttctgtttcc tgcggcgaga
    94861 tcatgcggac atttttcatg acctgggatt gcatcaccgt ctggctacca gagtcttccg
    94921 ggcgttgcca ggtctggatc tcctgatcgt taccgccaac cgtgcgccag ttggtaattg
    94981 caccactgaa gatgtcacgc acttgttgtt cggtcaggga attaaccggg ttatctgcat
    95041 tgacgatgaa aacaaacgct tcacgggcaa aaggcgtgta aatcaaagtg acgcccgatt
    95101 cctccgcgcg ttttttctgc ccaccggaag gttgagccac aaagataata tcggcattac
    95161 ctttaacaat cttgttatat gcttcgggag tacgagagtt cgccagatat tcccattcgt
    95221 gaaaatcttc cggcaaaacg cttagtgcat aaaaggcaga ggcataaatg gggtacgccg
    95281 ccgttgcgcc atcaagtcgc ggccagtttt gcgtgaactg aatttgcggt ttaccacgca
    95341 gtggtgtcag ttgattattg agtttatcgg gtcgccaggc ccaggtatca atattttcac
    95401 gcacaccgac gccgggatta agctgtttat ataaataagc ctggtaaccg gcgacaaaac
    95461 caagcatgag aaggataaac aagagagaat tacggcaacg taacccggac gtgcccgtta
    95521 aaggatatct ggtcggccag ccgtacccca gcgaaaaact gatatgcgcg ccaacaggta
    95581 taatgactaa ccaccagtac tcaccaccga ctaatgaggc taacaaaacg cccagccagg
    95641 ggagcataag atcagccaac caacccgctc ccatctggtg aaattgataa tctgccagat
    95701 gcagcgcgat tatccagacc cccagggtta ataatgcggg tactaaaaaa agacattgtc
    95761 gccagacggc tatttctctt ttttctggtg cactatcgtc cgcccacact cgccccgaga
    95821 aaaatagcat taatgcgccc agcgccagca taaaaatgtt gagcagaagg taaattgttg
    95881 gcgtgataaa gatattacaa acttcaataa taccggcaag tccgacaccc agcatggcaa
    95941 taaccgccgc caaaaactgc gccagaatgg ggatgccgaa aaaagtcatt accagcgagg
    96001 tcaaaatcca ttttctgttt tgcattattc tttccattct ttctgaatgg cgaaattata
    96061 ctccccgagt ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg
    96121 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc aatatgccag
    96181 atatgatcgg cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
    96241 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcatc gactttatcc
    96301 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
    96361 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
    96421 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
    96481 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaagataaa gatattgtca
    96541 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
    96601 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc
    96661 tgttcagaca gatcggccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
    96721 atgaacacga ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg
    96781 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
    96841 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
    96901 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
    96961 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
    97021 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
    97081 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
    97141 tcgtccagca cggctgaaag cgatgggttc gctaccgcta gccagcgacg cggcgtcaca
    97201 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
    97261 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
    97321 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
    97381 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
    97441 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
    97501 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
    97561 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
    97621 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
    97681 gttttatgca actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag
    97741 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
    97801 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
    97861 aatttaccga ggttaatttc gctactggct tgggcactcc acaaacgcaa cgtgttggtc
    97921 gcgtcggtat cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
    97981 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
    98041 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
    98101 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
    98161 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
    98221 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
    98281 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
    98341 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
    98401 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
    98461 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
    98521 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
    98581 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
    98641 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
    98701 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
    98761 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
    98821 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
    98881 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
    98941 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
    99001 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
    99061 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagacccgg taaggcttcc
    99121 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
    99181 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct
    99241 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
    99301 ttctcgtcca cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac
    99361 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
    99421 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
    99481 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
    99541 gcataaaaca tgccttgata ggccaagttg tgcacagtaa acaccgactt cgccggacgc
    99601 ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca
    99661 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
    99721 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
    99781 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
    99841 aacaacagcg tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggtc
    99901 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
    99961 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
   100021 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
   100081 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
   100141 cttcctctgc gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga
   100201 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
   100261 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
   100321 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
   100381 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
   100441 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
   100501 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
   100561 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
   100621 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
   100681 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
   100741 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
   100801 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
   100861 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
   100921 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
   100981 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
   101041 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
   101101 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt
   101161 tgatgcagtt agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg
   101221 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
   101281 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
   101341 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
   101401 ctgccagaca gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
   101461 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
   101521 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
   101581 aggttgagca tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt
   101641 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
   101701 accactgctt gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt
   101761 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg
   101821 ggagagcaac aacgttgtta acagggcgtg aatgctgtcg cgccgccgtt caaccaggtc
   101881 aagagaaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
   101941 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
   102001 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
   102061 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
   102121 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
   102181 cgggaaattt cccacctgat aaccaccagg agcgatatcc cacggttcag caattaactt
   102241 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
   102301 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
   102361 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
   102421 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
   102481 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
   102541 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
   102601 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
   102661 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
   102721 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
   102781 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
   102841 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
   102901 cgtgcgcggc ggggcatcat cttcccagtc atagtgatca accaccacta cgcatttcgg
   102961 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
   103021 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
   103081 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
   103141 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata
   103201 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
   103261 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
   103321 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
   103381 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
   103441 ccattgcctg cattactgcc gtgatagtgc atggaatcgg tattgaggat ttcacgccat
   103501 ttgcccggct ggtttatgcc gaagcgataa tcatgacgcg gtaccggcgt aaagttactg
   103561 gcaacgatga tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
   103621 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
   103681 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
   103741 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
   103801 tgggcaaatt cgttacccat gaacagtagt ttcttgcccg ggaatgccca catccagcca
   103861 tagtaggcgc gcaggttcgc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
   103921 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
   103981 ttgtagagaa tcccgaaggt cagtttatcg tgatgatact gacgataaac cgggtcgagc
   104041 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
   104101 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
   104161 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
   104221 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
   104281 tcgcgataaa tcattgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
   104341 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
   104401 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
   104461 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
   104521 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
   104581 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
   104641 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
   104701 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
   104761 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
   104821 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
   104881 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
   104941 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
   105001 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttaaattgc
   105061 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
   105121 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
   105181 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
   105241 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
   105301 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
   105361 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
   105421 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
   105481 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
   105541 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
   105601 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
   105661 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
   105721 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
   105781 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
   105841 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
   105901 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
   105961 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
   106021 cttccacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
   106081 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
   106141 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
   106201 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
   106261 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
   106321 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
   106381 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
   106441 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
   106501 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
   106561 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
   106621 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
   106681 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
   106741 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
   106801 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
   106861 ccataccgcg ccagccgata aaaccaacat ttttcataag cgtttttttc ctgcaaagat
   106921 gtgtgctgta taaatgtgcc ggtctcctct tggcacatct ttcaccatac aaaaagcagc
   106981 caaagtcgca agtgaaatta atcaatgata gcgaagccat cagtaatgcg acttatcctg
   107041 ttttgttagc acgcagaaag tccgcggcaa ttatcaggga atttgagtta tgaatgaaat
   107101 catttctgca gcagttttat tgatcctgat tatggatccg ctcggaaacc tacctatttt
   107161 catgtccgta ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg tgcgagagtt
   107221 gcttattgct ctcctggtga tgctggtgtt cctgtttgcg ggtgagaaaa ttctggcatt
   107281 tctcagcctg cgagcagaaa ccgtctccat ttctggcggc atcattctgt ttctaatcgc
   107341 cattaagatg attttcccca gcgcttcagg aaatagcagc gggcttccgg caggtgaaga
   107401 gccatttatc gtgccgttgg caattccgtt agtcgccggg ccgactattc tcgccacgct
   107461 gatgttgctg tctcaccagt acccaaatca gatgggacat ctggtgattg ctctgctgct
   107521 ggcctggggc ggcacctttg tcatcctgct acagtcttcg ctatttttac gtctgctggg
   107581 cgagaaaggg gtgaacgcac ttgaacgcct gatgggattg attctggtga tgatggcaac
   107641 ccagatgttc ctcgacggca ttagaatgtg gatgaagggg taaggttcaa acaggtgctg
   107701 cacacaacca gacgcggcac ctgttttatt acctgaactg ataatgacct tcgcccaatc
   107761 ggataaccct tccctcttct tctgcgcggt tcagccacac actaatttgt tcgatggtta
   107821 aaccagtaca agacgacagc ctttcaatac ttatcgattc ctttgcaaag atcgccagtt
   107881 ccgccacaaa gagttgataa aaatcgacag gcgtatgcgc gggcattact gcgtaatttg
   107941 cgtcattttc atgcagcgaa aacagttctg gttgagcgtt acggagtgct ctgtcataca
   108001 tcagcccagg aacgtctgac agtctgatcg gcacattctc agcctgttca gcactccatg
   108061 acgccccatt ttctactaat cgtgaattgg ctgattgcat atcctgattt ggtttaacct
   108121 gcacaggcag ccactgatgt tttaatgcct ctatcgcccc ggttatcgtt ccaccggtca
   108181 ttcccgcacg tacaaccatt gcgctttcag caaggcaata aatatattta tttcgcgcca
   108241 tcgcattgcc gacggtgaaa cgcacctctg ggtaaaacgg cgaaatcaac accaggttgc
   108301 ctgctataag cccttcacgc catttcacta acgtactcgt ttttaacaag ctatcagcta
   108361 atacgccaac ggcagttccc ccggcctcca gtgccgacgc cattgcacat tcatcaatac
   108421 ctcgcgcacc accagagata acgcaaatcc cctgttgggc cagtttcgcg gccagttgtt
   108481 gggtatagcg caaatcgtca gtcggagcat ctcgcgagcc aacaatcgcc ataccttccg
   108541 cttgcagtaa tgctttattc ccgcagccaa ataaaacggg aggtgcatcc gttcgcaatc
   108601 ggtttttcaa gcgaacggga taatcagcat ctcctcgggt tatgatccat aaaccggcgc
   108661 gttgccactt atctaccgcc aacgccagac tatgaccgcg cgccagcaag ccaagaatac
   108721 gctcctgaga aatacgcgga tcgctccagc ctgtaagttt ctcttgcggt tgcggcacca
   108781 gaagctcggc gggactgata cgttggtgct tcagccataa tgcaaacttc ccccattcac
   108841 tattactaag aggtttatgc tcactcgccg ccgcgcgaga aaaatcgctg gttagcagga
   108901 gagtcgcttg tgcattggct gaaagattca tcaatttttt accgaggtag aagcaagggc
   108961 aagaggataa accgtcgggc aacctgcctg gcgtaacagt gctgtcagaa ctgtcagcgt
   109021 ccatgcagaa tccacgatat cgtcaaccag caacgccgga cctggcatca aaggaggggt
   109081 aatcacaaac gccccgtcga gattttgaca ctggtgaaaa cggttttgct gcattttctg
   109141 cggtggattg tccacgactt tttcaatggc atcaataaaa ggtaagccaa gtttcgccgc
   109201 cagtcgccgg gcaaaatcag gaaccaggtc gaggtgcctt aatgaaggaa cgcagcatac
   109261 ccaggttggt tcaggatgcg gattccagcg ttggcgaacc atttccgcac atgcctccac
   109321 cagctcatca ctgaatcgcc ccgttttttt ctcctgtgct acctgctttc cccatccgga
   109381 atctccccaa cgagaaagga ctcgccccgt agatccttgt aaacccgcag gcaagttccc
   109441 tttaaatccg tattgcgtaa aagctccaga tgctacctgt ttattgagat ttaatggcag
   109501 gtcagcgtgt ttaataaata aatttgccgc atggagtaag ccgctatcaa tatcgggcga
   109561 caacaacgga tgctgaagac aactgctaca tttaccacaa cgttcggcac ttggctcatc
   109621 gagtgcgcgg cgcagaaatt gcattttgca ctccttggtc gtgatatagc tttcaacatc
   109681 tgccagctca ttttttctct gattcatcaa atggttaatt cgttcatggt caggagagaa
   109741 actgacgata gttctgcgcc ataatttctc ggtatacacc acaggcgatg gattttcagc
   109801 taccaataat ttcagtgctt tttctatttg cccataacgg agattcgtcc gttgttcaat
   109861 gcctcgtagg gtaagaccgt cattctcgct aagtacgttg agtatttcat gaatttgcgc
   109921 ctccgcagga aaggcgcttt cacggaagaa tttatgaata gcgcggtctt caccaccaca
   109981 aagcaatatg ccaacagcgg aatctatagc acgcccggca cgccctacct gttggtagta
   110041 accgacaata gaccccggca tctggtaatg aataacaaac cccaaatccg gtttgtcaaa
   110101 gcccattccc agcgccgtcg tcgcgaccag aactttaatt ttatttgcca gcaacgcctg
   110161 ctccagatat tccctggcag tattagaatc ttccgcgcct tcacaagtca cgccgctgta
   110221 gtaagcaaat gcactgatac catttgtcct taaccactcg gcgacaaggt cggcatcgcg
   110281 agtagtcagg gtataaacaa ttcccgattt ggaaaactga gggataaccg ttgctaacca
   110341 tgccagacgg gatgactgtt cacctaagac taaggcatca agcgccaaac tttcgcgagc
   110401 cagcgttcca cgctgaatca caatgtcacc caattgctga cggatatcct caacgacacg
   110461 gttattcgct gtcgcggttg tacccagaac tggggtattc gcaggcagtt ggcgcaaaat
   110521 atctaatata cgtcggtaat ccgggcgaaa atcgtggccc caatcagaga tgcagtgcgc
   110581 ctcgtcgacc acaagcaagc caatacgatc ggctatagga tataaaaccg tttcgataaa
   110641 gtcttggttt gccaaacgtt ccggggaaac cagcaagcaa tcgacctcac cttgcagcaa
   110701 cttatcgcta atacgctgcc attcttccct gtttgtagaa ttcaacgttt cagccgtaat
   110761 acctaaccgt tcagcggcgg caacctggtt acgcatcagg gcaagcaaag gagagatgat
   110821 aattgtcggg ccagcaccgc gatcgcggaa gattttactg gcgatgaagt acacggcact
   110881 tttcccccag ccagtgcgtt ggacaaccag cagttttcgt cgttggttca ccaccgcgtc
   110941 tatggcttcc cattgccctt ccctgaaagt agcggtggta tttcctaaca tccgctgtaa
   111001 aagcaattca gctgcgtgct tttccatctt tatctcctgc gatctttagg cagtgattag
   111061 aacggattat tagcaggtaa atttgcgaga gggatcaaaa agatgaagag aggagataag
   111121 agaaatgaat tactgcgagc aagctcgcag taatttgtcg cagtaattaa ctcaacagct
   111181 ggaacgcaat catcccaacg atggcaccga cagtgccgag gatggtttcc atcatggtcc
   111241 aggttttcag cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg
   111301 cgtcgttaac gtggctgaca acaatcgaac caccagcgat acaaatcgac agcgccgcca
   111361 tttgcgcacc ggagtagttc agttgttcaa taatcggcat caccagtcct accgccgtta
   111421 aacaggctac ggtggcagaa ccctgaatga tgcgcactgc agctgccagc acgaagcagg
   111481 tgatagcaat cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag
   111541 agtcaaccag cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg
   111601 ccggttgcag cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca
   111661 ttgccaggcc ataaatcgct accagacaag caaccagaat cgcggtaaac ggatgaccaa
   111721 taaactcgaa ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg gcaatggttt
   111781 tcagccccac cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaagatggca
   111841 ttttgccttc gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtagc
   111901 ggctgatgaa attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca
   111961 ggccaatcag gatcatccag ccaaaatcgg cgttcatctg cgatgccagc agcattggcg
   112021 ctggccctgg caccaggaac gccgccgctg ccgccacgcc tgcgaataat gggattacca
   112081 gcttcaccag gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa
   112141 caatcgccac ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat
   112201 aatgcgcgcg gctgtgaccg aaggatttaa gcattttgac ggcaatctga tcgactgcgc
   112261 cggtttcatg taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc
   112321 cgagggtgcc tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg
   112381 aaaaaaggcc agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct
   112441 tcatgactaa aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg
   112501 tagtcactac ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca
   112561 gcggttgatc gatatccacc accagtacat cagtttcgtc cgcacccggc tcctgcagcg
   112621 tttcaaactg cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc
   112681 ggctttcaat cacgtcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt
   112741 cacgcagcaa gtcgcgatag tgttttttca atgcagaaca gacgatcagt gacactttat
   112801 tagtgcgctg catagcaaac gcggcgtcgt tcagcgcctg caaccacggt ttgcgatcgt
   112861 cgtcattcag cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat
   112921 cgccatcaag aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc
   112981 cgctgcctga gacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag
   113041 tggtgccccc acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt
   113101 ccggccggac aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta
   113161 cttcaaattt gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca
   113221 ttttcggtgt cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc
   113281 ccatccgctc acgcggcgtc agcacgctcg ccagtcgtgg ctccatcacc tgaccaatgt
   113341 catgaccgtg gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac
   113401 attcaaacgc cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg
   113461 gatattcccg ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct
   113521 caaccatcac gctatacggc accaggcctg catccagcat cgcctgttcg tatcccttct
   113581 gtttgatgat agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc
   113641 gcgcaataat ggcggtagtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga
   113701 tatcgaggca cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa
   113761 tcatctttaa ggtgcgcggc gtgtgggtac gttcggtgag gatcaggccg tcgatattcc
   113821 aggacagcat ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgcgcca
   113881 gcatcgtctg ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga
   113941 aaacctggtt ggtgagagaa ggtaacagga cgccaatcgc ccggctggta gcgttagaga
   114001 ggatatcggg cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc
   114061 cgcgtagagc gacggaaacc tgctccgggt tacgcaaaaa gcggctgacc gtcattttgg
   114121 tcacgcctac acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga
   114181 aggtacaaaa gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga
   114241 aaacagtgct tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta
   114301 acgacgcggc aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc
   114361 tgcaaaaagg caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt
   114421 aacacttcgc tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga
   114481 ccatcgctgg tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc
   114541 cagacgcggc gttcagcggc aatctgatgc accgactgct catctttcag caacgcccaa
   114601 cgatacagtt ccatatcctg atgcactttc agcgaaccat ctcgcgcatc cggcgagagc
   114661 accagctgtt tgccctgcac ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca
   114721 ttttcttcag gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg
   114781 tactctgagt gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc
   114841 tgctctttat tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa
   114901 atttccatat ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg
   114961 ttaatcacgc gcagcgcgga aaagcccata aagttcggat cgtaatagtt ggcaaaagag
   115021 aaagtatgcc aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt
   115081 aagtagatca tttgtattcg ccccatgaat gatttcgatg ggcttagtgt ggacccgatc
   115141 tgcctgggat gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga
   115201 ggggccatca aacttactta gcgagagtta ctgtggaggg agaggcttgc tcaaatccgc
   115261 gttcaaggat ttccagattc gtaagaactt cagattcctt gacgtaattt ggcgcaccgt
   115321 gggtgatggt ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca
   115381 tctcttctct gaccgtcacg ccctcgtcat tgacatactc cagcacaccg accgaatcat
   115441 ccgctgcgaa tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt
   115501 cgataccgta tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga
   115561 ttttcaccag atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt
   115621 caaaggtgtc gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat
   115681 ccgggcgacc gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg
   115741 cgccatcctg cggcagccca ggtttggtct ctgccaccgg gcggtaatag tcaaaatggc
   115801 tttccacttc aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga
   115861 agcaggagtc aaagcgacga ttctgatacg gcgtgacggt cagccctttg cttttcgcca
   115921 acgcaaacag ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat
   115981 ttttcccggc ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga
   116041 caacaaccag cttaacatcg ggatcgttta gtacttcgtc gagatcgctg gtgaaatgga
   116101 tatgggaata aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga
   116161 catgccagct atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc
   116221 cgaagccaat aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta
   116281 caccttagcg caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc
   116341 ccgtggataa attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat
   116401 actggaagca atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg
   116461 cgagacaata ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat
   116521 ttgactcgca tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag
   116581 gaatgagtga gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga
   116641 ttcacgccca gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata
   116701 tgtggcagga gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg
   116761 gagacgtcgt gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg
   116821 ccgattttgg tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga
   116881 tgcgagagat gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg
   116941 tgtttgtcga taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag
   117001 ggactggtaa gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc
   117061 gggtgaagta agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac
   117121 ataccttcct ttaaggtatt tatttgccca atacatatat tgagatttaa ctcataatta
   117181 cttcctaaag tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat
   117241 aaataacaat taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt
   117301 ttcagggatg agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc
   117361 cgcaggttgt gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga
   117421 aatatttaca ttctcactct taaataatat taataatacg gggcttggtt cacagttcca
   117481 tggtataaca ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa
   117541 caataatgaa ca
//
LOCUS       DAZFYC010000002       112489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFYC010000002 DAZFYC010000000
VERSION     DAZFYC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 112489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 112489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..112489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.002"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..530
                     /locus_tag="ACHKW5_000108"
     CDS             126..530
                     /locus_tag="ACHKW5_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO7509571.1"
                     /translation="MSAGLTSESLNQWLSVSSLAAVIAGVPPEVALGSLAGAVIFVTS
                     AVEYPIRRRVLLSMLSFLCGLLFYKPAASILIGIASLIPTITQDSFEKGIVFSAGAFV
                     SAIVAVRIGIWLYHRSDNPRELIPGRKDDGNA"
     gene            517..825
                     /locus_tag="ACHKW5_000109"
     CDS             517..825
                     /locus_tag="ACHKW5_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7509572.1"
                     /translation="MVTHDLFLLITNAVICTGIAIRVVTFQRNGSQHRRWGGWIAYFL
                     IVAAASIPVRVVYAIWLRTPMAVDLSEVIINAVMLAAVIKTRGNVVQIFKITRSKHGD
                     "
     gene            815..1444
                     /locus_tag="ACHKW5_000110"
     CDS             815..1444
                     /locus_tag="ACHKW5_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HFO7509573.1"
                     /translation="MEIKQFQRAAGISEALAARWFSHITSAMKEFGISKTEDQAMFIA
                     QVGHESGGFTRLQENFNYSVSGLANFVRAGRLTQGQANALGRRAGEPPLPLERQRAIA
                     NLVYSKRMGNNAPGDGWNYRGRGLIQITGLNNYRDCGNGLKVDLLENPELLAQDEYAA
                     RSAAWFFSGKGCMKYTGDIARVTLIINGGRNGIDDRRARYITASKVLTV"
     gene            1441..1923
                     /locus_tag="ACHKW5_000111"
     CDS             1441..1923
                     /locus_tag="ACHKW5_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO7509574.1"
                     /translation="MILTFVKSYWKQLLIVLMLAALFIVGVVAWNIHGSRQYDAGYAQ
                     AEEDRKAEEDKVRQYYEQEKVTNEREAQQRIDQARSDALDAAARAGRLQRQLVAIREQ
                     LRQYNAIVGAGASAADTGVLLADVFSKSLERNRQLAEYADRAAEAGRVCEKQYDTLTR
                     "
     gene            complement(2142..2681)
                     /gene="yfgI"
                     /locus_tag="ACHKW5_000112"
     CDS             complement(2142..2681)
                     /gene="yfgI"
                     /locus_tag="ACHKW5_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO7509575.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(2697..3215)
                     /gene="yfgH"
                     /locus_tag="ACHKW5_000113"
     CDS             complement(2697..3215)
                     /gene="yfgH"
                     /locus_tag="ACHKW5_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7509576.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKKCQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(3526..3717)
                     /gene="yfgG"
                     /locus_tag="ACHKW5_000114"
     CDS             complement(3526..3717)
                     /gene="yfgG"
                     /locus_tag="ACHKW5_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO7509577.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            3735..3887
                     /locus_tag="ACHKW5_000115"
     CDS             3735..3887
                     /locus_tag="ACHKW5_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509578.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            4069..6312
                     /gene="pdeF"
                     /locus_tag="ACHKW5_000116"
     CDS             4069..6312
                     /gene="pdeF"
                     /locus_tag="ACHKW5_000116"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO7509579.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(6351..7892)
                     /gene="ppx"
                     /locus_tag="ACHKW5_000117"
     CDS             complement(6351..7892)
                     /gene="ppx"
                     /locus_tag="ACHKW5_000117"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO7509580.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(7897..9963)
                     /gene="ppk1"
                     /locus_tag="ACHKW5_000118"
     CDS             complement(7897..9963)
                     /gene="ppk1"
                     /locus_tag="ACHKW5_000118"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO7509581.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(10134..10772)
                     /gene="purN"
                     /locus_tag="ACHKW5_000119"
     CDS             complement(10134..10772)
                     /gene="purN"
                     /locus_tag="ACHKW5_000119"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO7509582.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(10772..11809)
                     /gene="purM"
                     /locus_tag="ACHKW5_000120"
     CDS             complement(10772..11809)
                     /gene="purM"
                     /locus_tag="ACHKW5_000120"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO7509583.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            12134..12760
                     /gene="upp"
                     /locus_tag="ACHKW5_000121"
     CDS             12134..12760
                     /gene="upp"
                     /locus_tag="ACHKW5_000121"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO7509584.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            12846..14135
                     /gene="uraA"
                     /locus_tag="ACHKW5_000122"
     CDS             12846..14135
                     /gene="uraA"
                     /locus_tag="ACHKW5_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO7509585.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            14230..14931
                     /gene="hda"
                     /locus_tag="ACHKW5_000123"
     CDS             14230..14931
                     /gene="hda"
                     /locus_tag="ACHKW5_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO7509586.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(15069..15428)
                     /gene="arsC"
                     /locus_tag="ACHKW5_000124"
     CDS             complement(15069..15428)
                     /gene="arsC"
                     /locus_tag="ACHKW5_000124"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO7509587.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(15449..16912)
                     /gene="bepA"
                     /locus_tag="ACHKW5_000125"
     CDS             complement(15449..16912)
                     /gene="bepA"
                     /locus_tag="ACHKW5_000125"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO7509588.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            17125..18186
                     /gene="yfgO"
                     /locus_tag="ACHKW5_000126"
     CDS             17125..18186
                     /gene="yfgO"
                     /locus_tag="ACHKW5_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7509589.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(18224..19075)
                     /gene="focB"
                     /locus_tag="ACHKW5_000127"
     CDS             complement(18224..19075)
                     /gene="focB"
                     /locus_tag="ACHKW5_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO7509590.1"
                     /translation="MHEKQTLFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGV
                     FMAIGFTFYLSVIADTPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSR
                     GVISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHT
                     FTESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIP
                     FAIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRA
                     IYLRQES"
     gene            complement(19093..21105)
                     /gene="hyfR"
                     /locus_tag="ACHKW5_000128"
     CDS             complement(19093..21105)
                     /gene="hyfR"
                     /locus_tag="ACHKW5_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO7509591.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHFDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRSMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(21135..21548)
                     /gene="hyfJ"
                     /locus_tag="ACHKW5_000129"
     CDS             complement(21135..21548)
                     /gene="hyfJ"
                     /locus_tag="ACHKW5_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO7509592.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(21541..22298)
                     /gene="hyfI"
                     /locus_tag="ACHKW5_000130"
                     /pseudo
     CDS             complement(21541..22298)
                     /gene="hyfI"
                     /locus_tag="ACHKW5_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(22295..22833)
                     /gene="hyfH"
                     /locus_tag="ACHKW5_000131"
                     /pseudo
     CDS             complement(22295..22833)
                     /gene="hyfH"
                     /locus_tag="ACHKW5_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
     gene            complement(22843..24510)
                     /gene="hyfG"
                     /locus_tag="ACHKW5_000132"
     CDS             complement(22843..24510)
                     /gene="hyfG"
                     /locus_tag="ACHKW5_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO7509593.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(24500..26086)
                     /gene="hyfF"
                     /locus_tag="ACHKW5_000133"
     CDS             complement(24500..26086)
                     /gene="hyfF"
                     /locus_tag="ACHKW5_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO7509594.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(26091..26741)
                     /gene="hyfE"
                     /locus_tag="ACHKW5_000134"
     CDS             complement(26091..26741)
                     /gene="hyfE"
                     /locus_tag="ACHKW5_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO7509595.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(26753..27983)
                     /locus_tag="ACHKW5_000135"
                     /pseudo
     CDS             complement(26753..27983)
                     /locus_tag="ACHKW5_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(28000..28947)
                     /gene="hyfC"
                     /locus_tag="ACHKW5_000136"
     CDS             complement(28000..28947)
                     /gene="hyfC"
                     /locus_tag="ACHKW5_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO7509596.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(28958..30976)
                     /gene="hyfB"
                     /locus_tag="ACHKW5_000137"
     CDS             complement(28958..30976)
                     /gene="hyfB"
                     /locus_tag="ACHKW5_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO7509597.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQSLRVVFSALFRLRQQFDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(30976..31593)
                     /gene="hyfA"
                     /locus_tag="ACHKW5_000138"
     CDS             complement(30976..31593)
                     /gene="hyfA"
                     /locus_tag="ACHKW5_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO7509598.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(31846..32316)
                     /gene="bcp"
                     /locus_tag="ACHKW5_000139"
     CDS             complement(31846..32316)
                     /gene="bcp"
                     /locus_tag="ACHKW5_000139"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO7509599.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(32316..32888)
                     /gene="gcvR"
                     /locus_tag="ACHKW5_000140"
     CDS             complement(32316..32888)
                     /gene="gcvR"
                     /locus_tag="ACHKW5_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO7509600.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            33034..33912
                     /gene="dapA"
                     /locus_tag="ACHKW5_000141"
     CDS             33034..33912
                     /gene="dapA"
                     /locus_tag="ACHKW5_000141"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO7509601.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            33929..34963
                     /gene="bamC"
                     /locus_tag="ACHKW5_000142"
     CDS             33929..34963
                     /gene="bamC"
                     /locus_tag="ACHKW5_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO7509602.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            35176..35889
                     /gene="purC"
                     /locus_tag="ACHKW5_000143"
     CDS             35176..35889
                     /gene="purC"
                     /locus_tag="ACHKW5_000143"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO7509603.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            36043..36270
                     /gene="vapB"
                     /locus_tag="ACHKW5_000144"
     CDS             36043..36270
                     /gene="vapB"
                     /locus_tag="ACHKW5_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO7509604.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            36270..36668
                     /locus_tag="ACHKW5_000145"
     CDS             36270..36668
                     /locus_tag="ACHKW5_000145"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO7509605.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            36815..37678
                     /gene="ypfJ"
                     /locus_tag="ACHKW5_000146"
     CDS             36815..37678
                     /gene="ypfJ"
                     /locus_tag="ACHKW5_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO7509606.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            37693..39708
                     /gene="tmcA"
                     /locus_tag="ACHKW5_000147"
     CDS             37693..39708
                     /gene="tmcA"
                     /locus_tag="ACHKW5_000147"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO7509607.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            39782..40480
                     /gene="ypfH"
                     /locus_tag="ACHKW5_000148"
     CDS             39782..40480
                     /gene="ypfH"
                     /locus_tag="ACHKW5_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7509608.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(40590..40790)
                     /gene="ypfN"
                     /locus_tag="ACHKW5_000149"
     CDS             complement(40590..40790)
                     /gene="ypfN"
                     /locus_tag="ACHKW5_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO7509609.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(40818..41945)
                     /gene="dapE"
                     /locus_tag="ACHKW5_000150"
     CDS             complement(40818..41945)
                     /gene="dapE"
                     /locus_tag="ACHKW5_000150"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO7509610.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(41949..42305)
                     /gene="yffB"
                     /locus_tag="ACHKW5_000151"
     CDS             complement(41949..42305)
                     /gene="yffB"
                     /locus_tag="ACHKW5_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO7509611.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            42686..42745
                     /gene="ypfM"
                     /locus_tag="ACHKW5_000152"
     CDS             42686..42745
                     /gene="ypfM"
                     /locus_tag="ACHKW5_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO7509612.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(42844..45957)
                     /gene="acrD"
                     /locus_tag="ACHKW5_000153"
     CDS             complement(42844..45957)
                     /gene="acrD"
                     /locus_tag="ACHKW5_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO7509613.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46121..47821)
                     /gene="narQ"
                     /locus_tag="ACHKW5_000154"
     CDS             complement(46121..47821)
                     /gene="narQ"
                     /locus_tag="ACHKW5_000154"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO7509614.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            48027..50006
                     /gene="aegA"
                     /locus_tag="ACHKW5_000155"
     CDS             48027..50006
                     /gene="aegA"
                     /locus_tag="ACHKW5_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO7509615.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            50074..50649
                     /gene="nudK"
                     /locus_tag="ACHKW5_000156"
     CDS             50074..50649
                     /gene="nudK"
                     /locus_tag="ACHKW5_000156"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO7509616.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            50775..51818
                     /gene="ypfG"
                     /locus_tag="ACHKW5_000157"
     CDS             50775..51818
                     /gene="ypfG"
                     /locus_tag="ACHKW5_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO7509617.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(51914..53917)
                     /gene="tkt"
                     /locus_tag="ACHKW5_000158"
     CDS             complement(51914..53917)
                     /gene="tkt"
                     /locus_tag="ACHKW5_000158"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7509618.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(53937..54887)
                     /gene="tal"
                     /locus_tag="ACHKW5_000159"
     CDS             complement(53937..54887)
                     /gene="tal"
                     /locus_tag="ACHKW5_000159"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7509619.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGIFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            55176..57455
                     /gene="maeB"
                     /locus_tag="ACHKW5_000160"
     CDS             55176..57455
                     /gene="maeB"
                     /locus_tag="ACHKW5_000160"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO7509620.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            57748..58083
                     /gene="eutS"
                     /locus_tag="ACHKW5_000161"
     CDS             57748..58083
                     /gene="eutS"
                     /locus_tag="ACHKW5_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO7509621.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVDLWLGGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            58096..58575
                     /gene="eutP"
                     /locus_tag="ACHKW5_000162"
     CDS             58096..58575
                     /gene="eutP"
                     /locus_tag="ACHKW5_000162"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO7509622.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLMDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            58550..59251
                     /gene="eutQ"
                     /locus_tag="ACHKW5_000163"
     CDS             58550..59251
                     /gene="eutQ"
                     /locus_tag="ACHKW5_000163"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO7509623.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            59248..60051
                     /gene="eutT"
                     /locus_tag="ACHKW5_000164"
     CDS             59248..60051
                     /gene="eutT"
                     /locus_tag="ACHKW5_000164"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO7509624.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            60048..61064
                     /gene="pta"
                     /locus_tag="ACHKW5_000165"
     CDS             60048..61064
                     /gene="pta"
                     /locus_tag="ACHKW5_000165"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7509625.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     PLGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            61103..61396
                     /gene="eutM"
                     /locus_tag="ACHKW5_000166"
     CDS             61103..61396
                     /gene="eutM"
                     /locus_tag="ACHKW5_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO7509626.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            61503..61790
                     /gene="eutN"
                     /locus_tag="ACHKW5_000167"
     CDS             61503..61790
                     /gene="eutN"
                     /locus_tag="ACHKW5_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO7509627.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            61802..63205
                     /gene="eutE"
                     /locus_tag="ACHKW5_000168"
     CDS             61802..63205
                     /gene="eutE"
                     /locus_tag="ACHKW5_000168"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO7509628.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            63216..64052
                     /gene="eutJ"
                     /locus_tag="ACHKW5_000169"
     CDS             63216..64052
                     /gene="eutJ"
                     /locus_tag="ACHKW5_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO7509629.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            64042..65229
                     /gene="eutG"
                     /locus_tag="ACHKW5_000170"
     CDS             64042..65229
                     /gene="eutG"
                     /locus_tag="ACHKW5_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO7509630.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            65346..66572
                     /gene="eutH"
                     /locus_tag="ACHKW5_000171"
     CDS             65346..66572
                     /gene="eutH"
                     /locus_tag="ACHKW5_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO7509631.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            66569..67972
                     /gene="eutA"
                     /locus_tag="ACHKW5_000172"
     CDS             66569..67972
                     /gene="eutA"
                     /locus_tag="ACHKW5_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO7509632.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            67984..69345
                     /gene="eutB"
                     /locus_tag="ACHKW5_000173"
     CDS             67984..69345
                     /gene="eutB"
                     /locus_tag="ACHKW5_000173"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO7509633.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            69366..70253
                     /gene="eutC"
                     /locus_tag="ACHKW5_000174"
     CDS             69366..70253
                     /gene="eutC"
                     /locus_tag="ACHKW5_000174"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO7509634.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            70263..70922
                     /gene="eutL"
                     /locus_tag="ACHKW5_000175"
     CDS             70263..70922
                     /gene="eutL"
                     /locus_tag="ACHKW5_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO7509635.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            70935..71435
                     /gene="eutK"
                     /locus_tag="ACHKW5_000176"
     CDS             70935..71435
                     /gene="eutK"
                     /locus_tag="ACHKW5_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO7509636.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPQQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            71481..72533
                     /gene="eutR"
                     /locus_tag="ACHKW5_000177"
     CDS             71481..72533
                     /gene="eutR"
                     /locus_tag="ACHKW5_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO7509637.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(72539..73438)
                     /gene="hemF"
                     /locus_tag="ACHKW5_000178"
     CDS             complement(72539..73438)
                     /gene="hemF"
                     /locus_tag="ACHKW5_000178"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO7509638.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(73442..74311)
                     /gene="amiA"
                     /locus_tag="ACHKW5_000179"
     CDS             complement(73442..74311)
                     /gene="amiA"
                     /locus_tag="ACHKW5_000179"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO7509639.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            74525..74950
                     /gene="ypeA"
                     /locus_tag="ACHKW5_000180"
     CDS             74525..74950
                     /gene="ypeA"
                     /locus_tag="ACHKW5_000180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO7509640.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            74937..75386
                     /gene="yfeZ"
                     /locus_tag="ACHKW5_000181"
     CDS             74937..75386
                     /gene="yfeZ"
                     /locus_tag="ACHKW5_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO7509641.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            75447..76022
                     /gene="yfeY"
                     /locus_tag="ACHKW5_000182"
     CDS             75447..76022
                     /gene="yfeY"
                     /locus_tag="ACHKW5_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO7509642.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            76118..77017
                     /gene="yfeX"
                     /locus_tag="ACHKW5_000183"
     CDS             76118..77017
                     /gene="yfeX"
                     /locus_tag="ACHKW5_000183"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO7509643.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(77075..78376)
                     /gene="pbp4b"
                     /locus_tag="ACHKW5_000184"
     CDS             complement(77075..78376)
                     /gene="pbp4b"
                     /locus_tag="ACHKW5_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO7509644.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(78381..79805)
                     /gene="murP"
                     /locus_tag="ACHKW5_000185"
     CDS             complement(78381..79805)
                     /gene="murP"
                     /locus_tag="ACHKW5_000185"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO7509645.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(79809..80705)
                     /gene="murQ"
                     /locus_tag="ACHKW5_000186"
     CDS             complement(79809..80705)
                     /gene="murQ"
                     /locus_tag="ACHKW5_000186"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO7509646.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            80869..81726
                     /gene="murR"
                     /locus_tag="ACHKW5_000187"
     CDS             80869..81726
                     /gene="murR"
                     /locus_tag="ACHKW5_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO7509647.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            81855..82646
                     /gene="ucpA"
                     /locus_tag="ACHKW5_000188"
     CDS             81855..82646
                     /gene="ucpA"
                     /locus_tag="ACHKW5_000188"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO7509648.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            82817..83833
                     /gene="cysP"
                     /locus_tag="ACHKW5_000189"
     CDS             82817..83833
                     /gene="cysP"
                     /locus_tag="ACHKW5_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO7509649.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            83833..84666
                     /gene="cysT"
                     /locus_tag="ACHKW5_000190"
     CDS             83833..84666
                     /gene="cysT"
                     /locus_tag="ACHKW5_000190"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO7509650.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            84666..85541
                     /gene="cysW"
                     /locus_tag="ACHKW5_000191"
     CDS             84666..85541
                     /gene="cysW"
                     /locus_tag="ACHKW5_000191"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO7509651.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            85531..86628
                     /gene="cysA"
                     /locus_tag="ACHKW5_000192"
     CDS             85531..86628
                     /gene="cysA"
                     /locus_tag="ACHKW5_000192"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO7509652.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            86762..87673
                     /gene="cysM"
                     /locus_tag="ACHKW5_000193"
     CDS             86762..87673
                     /gene="cysM"
                     /locus_tag="ACHKW5_000193"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO7509653.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(87676..88044)
                     /gene="yfeK"
                     /locus_tag="ACHKW5_000194"
     CDS             complement(87676..88044)
                     /gene="yfeK"
                     /locus_tag="ACHKW5_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO7509654.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            88149..89000
                     /gene="pdxK"
                     /locus_tag="ACHKW5_000195"
                     /gene_synonym="thiJ"
     CDS             88149..89000
                     /gene="pdxK"
                     /locus_tag="ACHKW5_000195"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO7509655.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(89042..89551)
                     /gene="crr"
                     /locus_tag="ACHKW5_000196"
     CDS             complement(89042..89551)
                     /gene="crr"
                     /locus_tag="ACHKW5_000196"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO7509656.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(89592..91319)
                     /gene="ptsI"
                     /locus_tag="ACHKW5_000197"
     CDS             complement(89592..91319)
                     /gene="ptsI"
                     /locus_tag="ACHKW5_000197"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO7509657.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(91364..91621)
                     /gene="ptsH"
                     /locus_tag="ACHKW5_000198"
     CDS             complement(91364..91621)
                     /gene="ptsH"
                     /locus_tag="ACHKW5_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO7509658.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(92005..92976)
                     /gene="cysK"
                     /locus_tag="ACHKW5_000199"
     CDS             complement(92005..92976)
                     /gene="cysK"
                     /locus_tag="ACHKW5_000199"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO7509659.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(93161..93922)
                     /gene="cysZ"
                     /locus_tag="ACHKW5_000200"
     CDS             complement(93161..93922)
                     /gene="cysZ"
                     /locus_tag="ACHKW5_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO7509660.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            94152..95138
                     /gene="zipA"
                     /locus_tag="ACHKW5_000201"
     CDS             94152..95138
                     /gene="zipA"
                     /locus_tag="ACHKW5_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO7509661.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            95209..97224
                     /gene="ligA"
                     /locus_tag="ACHKW5_000202"
     CDS             95209..97224
                     /gene="ligA"
                     /locus_tag="ACHKW5_000202"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO7509662.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            97226..97444
                     /gene="ypeB"
                     /locus_tag="ACHKW5_000203"
     CDS             97226..97444
                     /gene="ypeB"
                     /locus_tag="ACHKW5_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO7509663.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEVYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(97441..98439)
                     /gene="yfeH"
                     /locus_tag="ACHKW5_000204"
     CDS             complement(97441..98439)
                     /gene="yfeH"
                     /locus_tag="ACHKW5_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO7509664.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            98529..99455
                     /gene="yfeR"
                     /locus_tag="ACHKW5_000205"
     CDS             98529..99455
                     /gene="yfeR"
                     /locus_tag="ACHKW5_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7509665.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(99494..100258)
                     /gene="yfeN"
                     /locus_tag="ACHKW5_000206"
     CDS             complement(99494..100258)
                     /gene="yfeN"
                     /locus_tag="ACHKW5_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO7509666.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFSLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            100507..101340
                     /gene="xapA"
                     /locus_tag="ACHKW5_000207"
     CDS             100507..101340
                     /gene="xapA"
                     /locus_tag="ACHKW5_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO7509667.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHDHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            101400..102656
                     /gene="xapB"
                     /locus_tag="ACHKW5_000208"
     CDS             101400..102656
                     /gene="xapB"
                     /locus_tag="ACHKW5_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO7509668.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            102709..102867
                     /locus_tag="ACHKW5_000209"
     CDS             102709..102867
                     /locus_tag="ACHKW5_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO7509669.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMQ"
     gene            102908..103792
                     /gene="xapR"
                     /locus_tag="ACHKW5_000210"
     CDS             102908..103792
                     /gene="xapR"
                     /locus_tag="ACHKW5_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO7509670.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLRIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(103901..103976)
                     /locus_tag="ACHKW5_000211"
     tRNA            complement(103901..103976)
                     /locus_tag="ACHKW5_000211"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103941..103943),aa:Lys,seq:ttt)
     gene            complement(103981..104056)
                     /locus_tag="ACHKW5_000212"
     tRNA            complement(103981..104056)
                     /locus_tag="ACHKW5_000212"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104021..104023),aa:Val,seq:tac)
     gene            complement(104103..104178)
                     /locus_tag="ACHKW5_000213"
     tRNA            complement(104103..104178)
                     /locus_tag="ACHKW5_000213"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104143..104145),aa:Val,seq:tac)
     gene            complement(104223..104298)
                     /locus_tag="ACHKW5_000214"
     tRNA            complement(104223..104298)
                     /locus_tag="ACHKW5_000214"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104263..104265),aa:Val,seq:tac)
     gene            104557..105972
                     /gene="gltX"
                     /locus_tag="ACHKW5_000215"
     CDS             104557..105972
                     /gene="gltX"
                     /locus_tag="ACHKW5_000215"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO7509671.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APLPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(106024..106416)
                     /gene="yfeD"
                     /locus_tag="ACHKW5_000216"
     CDS             complement(106024..106416)
                     /gene="yfeD"
                     /locus_tag="ACHKW5_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7509672.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(106418..106777)
                     /gene="yfeC"
                     /locus_tag="ACHKW5_000217"
     CDS             complement(106418..106777)
                     /gene="yfeC"
                     /locus_tag="ACHKW5_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7509673.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            106998..107073
                     /locus_tag="ACHKW5_000218"
     tRNA            106998..107073
                     /locus_tag="ACHKW5_000218"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:107031..107033,aa:Ala,seq:ggc)
     gene            107113..107188
                     /locus_tag="ACHKW5_000219"
     tRNA            107113..107188
                     /locus_tag="ACHKW5_000219"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:107146..107148,aa:Ala,seq:ggc)
     gene            107396..109585
                     /gene="pdeA"
                     /locus_tag="ACHKW5_000220"
     CDS             107396..109585
                     /gene="pdeA"
                     /locus_tag="ACHKW5_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO7509674.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(109635..110837)
                     /gene="nupC"
                     /locus_tag="ACHKW5_000221"
     CDS             complement(109635..110837)
                     /gene="nupC"
                     /locus_tag="ACHKW5_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO7509675.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            111173..112411
                     /gene="mntH"
                     /locus_tag="ACHKW5_000222"
     CDS             111173..112411
                     /gene="mntH"
                     /locus_tag="ACHKW5_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO7509676.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
BASE COUNT        26878 a        28422 c        30223 g        26966 t
ORIGIN      
        1 tagataattg ctcctgttct ttcttccatt cataaaatta aattatgttt atccatatat
       61 ggtttgttgt gtatgatata cccactcaac taagggggtg tcatgaacag taaacggtgg
      121 ttgcaatgtc tgctggtcta acaagtgaat cattaaatca gtggcttagt gtgagttctc
      181 ttgctgcggt gatagcggga gttcctccag aggtcgcttt gggatctttg gctggtgcgg
      241 taatttttgt tacctctgca gtagaatacc caatccgtcg tcgtgttctt ctgtcgatgc
      301 tcagctttct ctgcggcctt cttttctaca aaccggcagc atcaattctt atcggcatag
      361 ctagcctgat tccaaccatc acgcaggact cattcgagaa agggattgta ttttctgcag
      421 gtgcgttcgt gtcggcaatt gtcgctgttc gtattggtat atggctctat caccgttccg
      481 ataatccacg cgagttaatt ccggggagaa aagacgatgg taacgcatga ccttttttta
      541 cttatcacca atgcagttat ttgtactggc atagcaattc gcgttgtcac tttccagcgt
      601 aacggatctc aacaccgaag atggggtggg tggattgctt atttcctgat tgttgctgcg
      661 gccagtattc ctgttcgtgt cgtttatgca atctggttac gcacgccaat ggctgtggat
      721 ttatctgagg tcattatcaa cgctgtcatg cttgctgcgg ttattaaaac gcgcggtaac
      781 gttgtgcaga tttttaaaat aacgaggtct aaacatggag attaaacaat tccagcgagc
      841 tgctggtatt agcgaggcgt tggccgcacg ctggttctcg catataactt ctgcgatgaa
      901 agagtttggt atcagcaaaa ccgaagatca ggcaatgttt attgctcagg tcgggcatga
      961 gtctggtggt ttcacccggt tgcaggagaa tttcaactac agtgtcagcg gtctagctaa
     1021 cttcgttcgg gctgggcgtc tcactcaggg gcaggctaat gcactgggtc gccgtgctgg
     1081 tgaaccacca ttgccacttg agcgccagcg cgcgatcgca aatctggtat acagcaaacg
     1141 catgggtaac aatgctcccg gcgatggctg gaattaccgt gggcgcgggc ttatccagat
     1201 taccggtttg aataactatc gtgactgtgg aaacggtctg aaagttgacc tgctggagaa
     1261 tcctgaactg ctggcgcagg acgaatacgc ggctcgtagc gcggcgtggt tcttctccgg
     1321 caaaggctgc atgaagtata ccggcgatat tgctcgtgta actctgatta tcaatggtgg
     1381 caggaacggc atagacgacc ggcgagcgcg atacatcact gccagtaagg tgctgacggt
     1441 atgatcctga cattcgtaaa atcatattgg aaacagctgc ttatcgtgtt gatgcttgct
     1501 gctctgttca tcgtcggagt ggttgcctgg aatatacacg gcagccgcca gtacgatgcc
     1561 gggtatgcgc aggcggaaga agaccgcaaa gccgaagaag acaaagttcg tcagtactac
     1621 gaacaggaga aagtgaccaa tgaacgtgaa gcgcagcaga gaatcgatca ggcgcgcagt
     1681 gatgctcttg atgctgccgc tcgcgctggc aggttgcagc gacagctcgt tgccatccgt
     1741 gagcagctca ggcaatataa cgccattgtc ggcgctgggg cgtcagccgc agataccgga
     1801 gttttgcttg ccgacgtgtt cagcaaatct ctcgagagaa atagacaact ggcagagtat
     1861 gctgaccggg cagccgaagc cggaagagtc tgcgaaaaac agtatgacac cctgaccaga
     1921 tagcatggta tttttcatgg tactgatttc cggtgacggt atataaaacg gtacgggaaa
     1981 aattgagctt tggaaaaatg ttaccactca attggttatg gtatccgtaa ataattgagt
     2041 ggaatgattt taatccctgc actatgaatg aacaaaaccc tctgttacta cagagggttt
     2101 tttatcttca agaatcatag gcttgaagtt actaacatcg attaattaaa ccagctgtcc
     2161 gatttgttct cttctgcttt gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata
     2221 tttctgttgg cgtcagcttc agattgcacc acatcggttt gcgcagcttt gtgcttcagt
     2281 tcctgatcga taaattcgtt ttctcgctta acgcgggctt cttcttttgc cagcgccagt
     2341 ttttgtttct gaatctctaa gctgcgtagc tcatcttcat aactttgatc gcgttttttg
     2401 tccgcagagg cttcggcgtc cagtttatcc tgacgagctt tcttatttgc tgctgccgtt
     2461 gccgctcttt tgttagcggc ggcctgggca tttgcgcgac gttgcttctc ttgctggatt
     2521 tccctgttgc gctccgcgac ccattcgtca tgctgctttt gctcttcatt tttaccttgc
     2581 tgttccgctt ctgcgacagc cgagagttga tcctgcaatg atgaggcgat agccggatag
     2641 cttaaggagg ccaagatggc gcaaagaaaa actttcttca tgactcctcc tgattattag
     2701 ctcttttcag gacatttagt atttggctga atacgcgttt cgttatacgt cgtggtaata
     2761 acaacggcta aacctgtcgt aaactggcac tttttaccca cctgggtgga ggtatacact
     2821 ttggtgcctt ccttatacgt taaagaaaca ccttccacta aggttttatc attcaccata
     2881 gaacccgctg ccgcgcctac agctccgccg ccaactgccc ctgccgtcgt tccggaattg
     2941 ctgccagaac cgacgttgtg gccgataaca ccgccagcga ctgcgccaat aagcgcgccg
     3001 aaggcttgtg cgttccgttt attttgggag ttgtctacgg caacttttgc gggaagaatg
     3061 gaaataatat taacggtttt agtttcttgt ttggtattca gttgatcggt ttgataaaca
     3121 tcggcggcat gatcgtcagc atttgactgg catcctgcca gagtgaatga cgctaacatt
     3181 gctacaggca gaagacattt tttaaatttc atcactattc cttgttatac acattacgaa
     3241 aaataagaca tttaagatca tcaaaagagg aaagtaaatg tattgcgtat aaagggatag
     3301 attaaattta ttaaaattca cgccgggaat aatttataaa aatatatcat tacaacatat
     3361 atattaattt tatatttatt tcgcagccaa acaggataaa catcagtcag gacaaagacg
     3421 taaaatttaa gatgaaagaa tatctgccgc tttgataatc ccaaagagca aaagcatccg
     3481 acagcgaagt gccgttaggt tctgtgaaag tggagaaggt aaccgctaac gttgtaccgg
     3541 tgattcgacg gagagtgtgg attgctgagc ttcttttttg ctctgatggt gctgccaggc
     3601 accgacggac gagtagataa aacggccaaa gaagaagata aagctgatga gcagtacgat
     3661 acgggtcatg cgactgttaa atcggtgtcg ttttcgcata ctggttgcct gactcacaaa
     3721 aggttccttg aagtatgtcc cacgctgtgg acggtactta cattaaggca caacaggaca
     3781 aaatggtcaa ttcttcgtta tgtaaaaaag cgtcagtgga tacatatttt aatgttatgg
     3841 aagttaattt aattatttac aatgatgatg taataatgat gaattaacat gataaacgta
     3901 ataattcatt aatcagggtt attttgtttg atatatatca attggaactt tcatatgaca
     3961 cttagaatca atgctctcta tagtgatgaa taatcatcat tcgaagtcag gtgggatgcc
     4021 tgtctgaata cacctccttc aggatgtggg ggattctgct gagcatctat gaaactgaat
     4081 gcaacttata taaaaatacg tgataaatgg tgggggcttc cgctgttcct gccttcttta
     4141 atcttgccca ttttcgccca cattaatact ttcgcgcata tttcttccgg tgaggttttt
     4201 ctcttttatc tgcctttggc actgatgatc agcatgatga tgtttttcag ctgggcggca
     4261 ttgccaggga tcaccttagg gatttttgtc cgcaaatatg cagagctggg tttttacgaa
     4321 acgctctcat taacggctaa ttttattatc attatcattc tctgttgggg cggttacagg
     4381 gtctttactc cccggcgtaa caacgtttca catggtgata cccgtttaat ttcccagcgt
     4441 atattctggc agatagtgtt tcctgcaacg ctgtttctga tacttttcca gtttgctgca
     4501 tttgtaggat tactggcgag cagagaaaat ctggtcggtg tcatgccttt taacctcggg
     4561 actttaatca attatcaggc cttactggtg ggtaatctga tcggtgtccc gctgtgctac
     4621 ttcatcattc gggtagtgcg aaatccattt tatttacgta gctattattc gcaattaaaa
     4681 cagcaggttg atgccaaagt caccaaaaaa gagttcgcgc tctggctact ggcattaggt
     4741 gctttattgt tgctgttatg catgccgtta aatgaaaaaa gcacaatttt tagcaccaat
     4801 tataccttgt cattattgtt gcccctgatg atgtggggag caatgcgcta tggttataag
     4861 ctgatttcgc tgctctgggc ggtcgtgttg atgatcagca tccacagcta tcaaaattac
     4921 attcccattt atcctggcta taccacgcag ctgaccataa cctcctccag ttatctggta
     4981 ttctctttta ttgtcaatta tatggctgta ctggcaactc gtcagcgagc ggtagtcaga
     5041 cgcattcagc ggcttgcgta tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg
     5101 aatcgcgcgt tgcgtgatgc cccctggtct gcgctttgtt atttacgcat ccctggcatg
     5161 gaaatgctgg ttaagaacta tggcatcatg ctgcggattc aatacaagca aaaactttct
     5221 cactggctgt cacccttgct ggaaccgggt gaagatgttt atcagctttc gggtaacgat
     5281 ctcgcgctgc ggctgaatac agaatcgcac caggagcgca ttaccgcact ggatagccat
     5341 ctcaagcaat ttcgtttctt ttgggatggc atgccgatgc aaccgcagat tggcgtcagt
     5401 tactgctatg tgcgctcgcc agtgaatcat atctacctgc tgctgggaga gctaaatacg
     5461 gtcgccgaac tttccatcgt gaccaacgcc ccggaaaata tgcagcgtcg cggggcaatg
     5521 tatttgcaac gcgaattgaa agataaagtc gcgatgatga atcgactaca gcaggcgctg
     5581 gaacacaacc attttttcct gatggcccag ccgattaccg gtatgcgtgg tgatgtttac
     5641 catgaaattc ttctgcgcat gaaaggtgag aatgatgaac tgatcggccc cgatagcttc
     5701 ttgccggtcg cgcacgaatt tggtttatcg tcgagtatcg acatgtgggt cattgagcat
     5761 acgctgcaat ttatggctga aaacagagcg aagatgcccg ctcaccgttt tgctattaat
     5821 ctgtctccaa cctcggtatg tcaggctcgt tttcctgttg aagtcagtca gttgctggct
     5881 aaatatcaga ttgaagcgtg gcaacttatt tttgaagtca ccgaaagtaa tgctctgacc
     5941 aatgttaagc aggcgcaaat caccttgcag catcttcagg aattaggctg ccagattgcg
     6001 attgatgatt tcggcaccgg ctacgccagc tatgcacggc ttaaaaatgt gaatgccgat
     6061 ctgcttaaaa ttgacggcag ttttatccgc aatattgtgt caaatagtct ggattatcag
     6121 atagtggcat cgatttgcca cctggcgcga atgaagaaaa tgcgggtagt ggcagagtac
     6181 gttgaaaacg aagagatccg cgaggcggtg ctctctttgg ggatcgatta tatgcagggt
     6241 tatcttattg gtaagccgca gccgttaatt gatacgttga atgaaatcga acccattcgc
     6301 gaaagtgcct gaataatgcg ggccgacatt tctcgtcggc ccgcaaagca ttaagcggcg
     6361 atttcaggtg tactttcttc ttcaattttc aaccgccagc cagccacgcc ttcccagtat
     6421 tcttgctcct tttccagatc aagcagtacc agcgcattct gactaaacca gtcatgcggg
     6481 aaacgcagtg tccagtgact gtcatcagta atcagtgtca atgttggcgg tgtggtggtt
     6541 gcctgacgtt gattgtttag taatacgcca aggcgcaata gctgtatcag tggcaggaac
     6601 tgtttcttct taaacaaggt aaagcgcggc agatcgtcga gcttaatcgc tttacggtga
     6661 tagcgcacca gtgtcgccat catcagctgc tgttcctgat taaaacccgg caagtcactg
     6721 ttttgcagaa tataagcgga gtggcgatgc aaaccgctgt ggttgatatt caacccgacc
     6781 tcatgcagca tggcggccca tcgcagtagc gcctccagtt gcggatgcgc cagcttcggt
     6841 tgctgttccc gccactgttc gtacatttgc atagtggtat ccagcactcg tcgggcctgt
     6901 tcgctgtcga tgtgatactg gttggcgagg ctgctggcgg tgcgactacg cacatcctga
     6961 tgacggaaac gtccttccat ttcatacagt acgccttcgc gtaacgcccc gtcagaaagg
     7021 cgcagttcac ggatggctaa agcatcaaac acaccgcata aaatcgccag tccaggaacg
     7081 aagactgttt tccgctcttc ggaaagaccc ggtaaactca gcgatgcgaa attacggtga
     7141 cgtaaaactt cttttaccag tttttccaga cgttccgggg taattatccc gtctttctcg
     7201 cccatttcca ttaacacttc atgggcggct tttatggtac cggaagcgcc cattgccacg
     7261 ttccagccct gaatacggaa ttgccaggtt aaagtttcca gtttttgtgc tgccgccata
     7321 cgagcgcgct gaaaattctc tttattgatg accccgccag ggaagtaaag ctgggcaaag
     7381 ctgacacaac ccatccggcg gctttcaacg agaatcggtt cgaaattttc accaatcacc
     7441 agttccgtag atccgccgcc aatatcaata accagtttgc gacctttctc cggttgggta
     7501 tgttccacgc ccataaaaat cagacgggct tcttcattac cggaaataat ttcaatcggg
     7561 tagggaatga ccttttccgc gcgtttcaga aagtcagtgg cgttcagcgc ctgacgcagc
     7621 gtatgggtgc cgactataca gacgctggca ggagaaaacc cttgtagccg ttcggcaaac
     7681 agcgacagac agttcaaacc gcgcgtcatt gcctcttcac tcaacatatt atctggcccc
     7741 aggccgtccg ccagatgcac ccgctgtttc aggcggccaa taatctgcat ggcaccatct
     7801 accacacggg ctatgaccat gtgaaaactg tttgaaccaa gatcgaccgc agcaaactcc
     7861 tgcggacgag gggatttatc gtgtattggc atagggttat tcaggttgtt cgagtgattt
     7921 gatgtagtcg taaatcgcca actgcgcccg tactttgcgg cgattgccgc ggggaacgta
     7981 gcgattactg agttctttat cgatataacg tgctttgacc gtatcgctga acaatatgtc
     8041 gatgatgtcc agcacccgct gcttcaggcg cggatcgagc agcggtgtcg ccacttcaat
     8101 acgataatca atattgcgcg tcatccagtc ggcggaagaa aggtagacct ttttatcgcc
     8161 gccattttca aaaatataaa cccggtcatg ttcaaggtaa cggtcaacaa tactgatggc
     8221 acgaatgttg tcgctaatgc cttccagatt ggggatcagc gaacacattc cgcgaaccag
     8281 cagattaacc ggtacgccgg agctggaggc cgcatacaga cgatcaacca ggcctttatc
     8341 gacaaggtta tttagcttca gggtgatacc actgggcagc ccttgctgcg cgttggcgat
     8401 ttcgcggtct accatttcat ataacagacg gcgagagttt tgcggtgaca ccattaaata
     8461 atcaaatgtc accgggcggt atgggttttc aataaagtta aatacccgcc gtacttcgtt
     8521 ggtgatacgc gcatctgcgg tcagcaacga atagtcagta taaagacgcg cggttttttc
     8581 gttaaagttc ccggtcccga tgtgtgcgta acggaccact tcaccgtttt ctttacgtga
     8641 aatcaggaac agtttggcgt gaattttcag ccccggcgca gagaagataa cgtgcacgcc
     8701 tgcttcggtc aggcgcttcg cccagtgaat gttggcttct tcgtcgaaac gcgcctgtaa
     8761 ctcaaccacc acggtgactt tcttaccgtt atgtgcggcg tggatcatcg agtcgatgat
     8821 gcgtgaatct ttcgccacgc ggtaaatgtt aattttaatc gccagtacgc tcgggtcgaa
     8881 cgaagcctga cgcagcagtt ccagcacatg ctcaaaggtg tgataaggat aatagagcaa
     8941 cacatcgcgt tcgcgaatgg catcaaaacc attgcggaac tgggctttat caaaccaaat
     9001 gtggcgtaaa cgcggcagtg gtttgttcac cagattggct ttgccgacat tggggaaatt
     9061 aataaagtct ttaaaattat gataacgacc gccggggacg atggagtcgt agcgggaaat
     9121 agtcagtttt tcgcgtaaca cttcaaccag cgcattgggc atatcgcgct gataaacaaa
     9181 acgcaccggc tcagcagtta aacgctgctt gagactggaa gacattaact ccatcaggct
     9241 ggcttccatc tcatgcacta aatcgtattc ggcatcgcgg gtcatcttca ttgaataggc
     9301 attcagcgcg tcataatcaa agaagccttt gaaaatatca tcgaggcagt aacgcagaat
     9361 gttatccaaa agaatcatcg gcttgcgtcg acgcggtgct tctggcggta aattcacaaa
     9421 gcgcggcact ttatctgatg ggatctccag cagcgcgtaa cggatggtat cgccacggat
     9481 aatttccacc gccagatagg tgtaatcatc tttcaggaac tgcactaagt cagtgtcagg
     9541 attgattaaa atcggcgtaa tgtgctgacg cagatactgc ttaaaataat gacgcagcca
     9601 gttttgttga ttgacggaga gctggcgttc attaatcagg aagatctggt tgcgcgccat
     9661 ctccagcaat agctcgttgt agaggccgtc gaattcctga tcggctttca gcacccggga
     9721 ctgaattttg cccagtaaat ggcgggaatg agagttggag ccttgttctt cgctaataat
     9781 gatgcgtcgc ttcagttcag cgaagcggac tttatagaac tcatcaaggt tattggaata
     9841 gatccccagg aaacgcatcc tttcaatcag cgggttagat ttgtccgccg cttcctgaag
     9901 cacgcgttca ttgaacgata accaactgag ctctttttcg atgtatagct tttcctgacc
     9961 cattaccact tttactccgt tttattcacg ggacactgcc tggatattat ggcgagcaat
    10021 attggcagat gtccaactat gccagaaaag ggggaaaaaa actgctggag cttgcgattt
    10081 acaggtgaat tgaaaaacgc cagcggcaga gctggcgctt taactacggg gcattactcg
    10141 tcggcagcgt agccctgcgg cggcagacgt tgaccatcca gccacgcggc gttttcgtgc
    10201 attttcagac gaccatcggc aaaccagcta atcaccagtg gataaatagc gtgttcctgg
    10261 gtttgcacgc gggcggtgat gtcatcttcc gtatcaccag caaataccgg gactttcgcc
    10321 tgtaaaataa ccgggccacc gtccagttca tcggtgacga aatgcaccga tgtaccgtgc
    10381 tcttcatcac cattttccag cgcctgacga tgggtgtgta atccgggata tttcggcagc
    10441 agagaagggt gaatgttcag caaacgcccg gcatagtggg agacaaacgc cgggctgaga
    10501 atgcgcataa aaccagccag cacgaccaca tcgggtgcgt acatgtcgat ttcatgaatc
    10561 aactcccggt cataggcttc acgactgtca aacgcgctgg cgatgagcgt atgcgttgca
    10621 atacccgcct ggcgggcgcg ttcaaggccg aacgcgtcgg ccttattgct gaaaactgcc
    10681 cgtacggtgc ctttaatttt gttagtttta caggcgtcaa taattgcctg taaattactt
    10741 ccgttgccgg aaataagcac cacaatattc attattcgat aaccacgcgt tgttcggaat
    10801 cagaggcttt gatgataccg attttccacg cgttttcacc gttggagttt agcagggcga
    10861 gggctttgtc cacttccgga gcaggcaggg cgataatcat cccgacgccg cagttgaagg
    10921 tgcgatacat ttcatggcgc tcaacgttac ctgccgtttg cagccagttg aacacttccg
    10981 gccactgcca ggaagattca tcaatcactg cctgagtatt atctggcaat acgcgtggaa
    11041 tgttttccca gaagccgccg ccggtcaggt gcgcaatggc atgcacatcg accttttcaa
    11101 tcaactccag cactgacttc acgtaaatgc gggtcggtgc cagcagatga tcggctaatg
    11161 gcttaccatc aagttcggtg gtttgcggat cacaaccgct gacttcaaga attttgcgca
    11221 ccagcgaata gccgttcgag tgcggaccgc tggaaccgag tgcaatcagc acatcgccgt
    11281 cgctgacttt agagccgtcg atgatttctg atttttctac cacgccaacg cagaaacccg
    11341 cgacatcgta atcctcaccg tgatacatcc ccggcatttc tgccgtttcg ccacccacca
    11401 gtgaacagcc cgattgcaga catccttccg cgataccgct gatcaccgct gaagcggtat
    11461 caacatccag ttttccggtt gcgtaatagt cgaggaaaaa cagcggctct gcaccttgca
    11521 ccaccaggtc attaacgcac atggcgacca gatcaatacc aatggtgtcg tgacgtttta
    11581 agtccatcgc cagacgcagc ttggtaccta cgccgtcagt accggaaacc agcacaggtt
    11641 cacgatattt ttgcggcaat gcacacagcg caccgaagcc gccaagaccg cccatcactt
    11701 ccggacgacg cgttttcttc actacgcctt tgattcttcc aaccagagca ttacccgcgt
    11761 caatatcaac accggcatct ttgtagctaa gagaggtttt atcggtcact gcttgggtcc
    11821 ccacgcgtta cttgcggtag aaaaataaaa ttcggcgcaa ttctaacagg gaaagcaaac
    11881 gtttgcgaga ctgctttaca caaccttttt gcacgtcttt tgcccaggca cgcggcgaaa
    11941 gaagacttgt gccagggtaa aggttagttt tcggatggaa taatcttctt tcataaccat
    12001 ttgaatataa aataacttta tctcaaaccg ttatcatttt gactaaagtc aacgaaaaga
    12061 atattgccgc cttgaagaaa ggaggtataa tccgtcgatt ttttttgtgg ctgcccctca
    12121 aaggagaaag agtatgaaga tcgtggaagt caaacaccca ctcgtcaaac acaagctggg
    12181 actgatgcgt gagcaagata tcagcaccaa gcgctttcgc gaactcgctt ccgaagtggg
    12241 tagcctgctg acttacgaag cgaccgccga cctcgaaacg gaaaaagtaa ctatcgaagg
    12301 ctggaacggc ccggtagaaa tcgaccagat caaaggtaag aaaattaccg ttgtgccaat
    12361 tctgcgtgcg ggtcttggta tgatggacgg tgtgctggaa aacgttccga gcgcgcgcat
    12421 cagcgttgtc ggtatgtacc gtaatgaaga aacgctggag ccggtaccgt acttccagaa
    12481 actggtttct aacatcgatg agcgtatggc gctgatcgtt gacccaatgc tggcaaccgg
    12541 tggttccgtt atcgcgacca tcgacctgct gaaaaaagcg ggctgcagca gcatcaaagt
    12601 tctggtgctg gtcgctgcgc cagaaggtat cgctgcgctg gaaaaagcgc acccggacgt
    12661 cgaactgtat accgcatcga ttgatcaggg actgaacgag cacggataca ttattccggg
    12721 cctcggcgat gccggtgaca aaatctttgg tacgaaataa agaataaaaa taattaaagc
    12781 cgactttaag agtcggcttt tttttgagta aagcgcctat aacacataat acagaggata
    12841 atactatgac gcgccgtgct atcggggtga gtgaaagacc gccactttta cagacaatcc
    12901 cgcttagttt gcaacatttg ttcgccatgt ttggtgcaac cgtcctggtg cccgtcttat
    12961 ttcatattaa cccggcgact gtactgttat ttaacggtat tggaacgctg ctgtatctct
    13021 tcatctgtaa agggaaaatt ccggcttatc ttggttccag ctttgccttt atttcaccgg
    13081 tattgttact gttgccgtta gggtatgaag tcgcgctggg cggctttatt atgtgcggcg
    13141 tgctgttctg cctggtttct tttatcgtga agaaagcggg gaccggctgg ctggacgtgc
    13201 tgtttccacc tgcggcaatg ggcgcaatcg ttgccgtcat cggtctggag ctggcgggcg
    13261 tagctgccgg tatggcgggt ttactcccgg ctgaagggca aacgccagac tccaaaacca
    13321 tcatcatctc tattaccacc ctggcggtca cggttttagg ttccgtgctg tttcgtggtt
    13381 tcctggcaat tatcccgatt ttaattggcg tgctggtggg ctacgcgctc tctttcgcaa
    13441 tgggaattgt cgataccacg ccgattatta atgctcactg gtttgcgctg ccaaccctct
    13501 atacgccgcg cttcgagtgg tttgccattc tgactattct gccagcggcg ttagtggtta
    13561 ttgccgaaca cgtagggcac ctggtagtaa cggctaatat cgtcaaaaaa gatctgctgc
    13621 gcgatccagg tctgcaccgt tcgatgtttg ctaacggcct gtcgaccgtg atctccggct
    13681 tctttggctc tacgccaaat accacttacg gagaaaacat tggcgtgatg gcgatcaccc
    13741 gtgtttacag tacctgggtt atcggtgggg cggcgatttt cgctatcctg ctttcctgcg
    13801 tcggtaaact ggctgccgct atccagatga tcccattgcc ggtgatgggc ggcgtttcgc
    13861 tgctgcttta tggtgtcatc ggtgcttccg gtattcgtgt tttgatcgaa tcgaaagtgg
    13921 attacaacaa agcacagaac ctgatcctga cttccgtgat tttgatcatc ggcgtcagtg
    13981 gcgcgaaggt aaacatcggc gcggcggagt taaaaggtat ggcgctggcg accatcgtcg
    14041 gtatcggctt aagtttgatt ttcaaactga tcagtgtgct gcgccctgaa gaagtggtgc
    14101 tggacgcaga agacgcggat ataacagaca aataatgtac cgaccgggca gtgttgctgc
    14161 ccggttcaaa catcatggga ttctgtggta aacttctcgc gattttgtga aatcctggtt
    14221 gaggtatctc tgaacacacc ggcacagctc tctttgccac tttatcttcc tgacgacgaa
    14281 acctttgcaa gtttctggcc gggggataac tcctctttac tggccgcgct gcaaaacgtg
    14341 ctgcgtcagg aacatagcgg ttacatctat ctctgggcac gcgaaggcgc ggggcgcagc
    14401 catctgctgc acgcggcttg cgcggaattg tcgcagcgtg gcgatgcggt gggctatgtc
    14461 ccgctggata aacgcacctg gtttgttccg gaagtgctcg acggtatgga gcatttgtcg
    14521 ctggtctgta tcgacaacat tgagtgtatt gcaggcgatg agttgtggga gatggcgatt
    14581 ttcgatctct acaatcgaat tctggaatcg ggcaaaacac ggttgttgat caccggcgat
    14641 cgtccaccgc ggcagttgaa tctgggatta ccggatctcg cgtcgcgact cgactggggg
    14701 cagatctaca aattgcagcc actttctgat gaagataagt tgcaggcgct acagttacgc
    14761 gcgcgtttgc gtggttttga actgccggaa gatgtggggc gtttcttgct gaagcggctc
    14821 gacagagaaa tgcgcacgct atttatgacg ttggatcagt tggatcgtgc gtcgattacc
    14881 gcgcaacgta agctgaccat tccgtttgtg aaagaaattc tgaagttgta gataaggtgt
    14941 ttattgtcgg atgcggcgcg aacgccttat ccgaactacg cgaagttaat ccatagtatt
    15001 cgtaggccgg ataagacgtg gcaagcgtcg catccggcaa tgtgcttcaa ctagcgatgg
    15061 agcacatctt aacccacgat ttccagtacc tgctccggtg gacgaccaat ccgcgctttg
    15121 ccattcgcga ccacaatcgg gcgctccatt agcttcggat tatccaccat tgcctgaatc
    15181 agcgcctctt cgctaagcga actgtcagcc aggttcagtt ctttataaag atcctctttc
    15241 tggcgcatca attcacgggc gctattcatc cccagcattt tcagcaaatc gcgcagcgtt
    15301 gccgcatcgg cgggtgtctc aaggtagagt accacttctg gctccacgcc gttttctttc
    15361 agcaggttca gcgtttcccg gctctttgaa cagcgtgggt tatggtagat ttttacctgt
    15421 ttggtcattg ttcttccttt aatgcgaatt acatcttggt ataaggctta aagcgttcct
    15481 gcagctggcg caactggtcg atgcgcgcat cgtaacgcgc ttgttgcagg ctgcctaatt
    15541 tcacctgcga actggcgcta ctcaacagcg aaatggcctg atcgagtcgt ccggcgagcg
    15601 cataaccttc tgctcgcgca gccagctcct gatcgcggtt atttagcgcg gcttccgcct
    15661 gtgccagtaa atcccagccg ttgctgtcat ctttattatt aaaggtgtag cgattcagaa
    15721 tattggccgc ttcttgtggt tgaccgcctt gcagataagc gttcgccagg ttgagctgca
    15781 acaccggatt ggtgcgtaaa tcgcgggcat ttttcagacg attgatcgcc tcattggctt
    15841 tgttttgccc aagatcgata tcagtagcca gatcgagata ccatgcgttg ccaggttctg
    15901 ccgccagtaa cggttgcagc gttttacgcg cctcgtcgta tttattggct tccatcgcct
    15961 gtaaagcacg accatattgt gctgctcgtt gctgacgaac gtttcctttc gcccattcat
    16021 ctagcaaatc actggtgagc tggttacgtc cggaattata catccccagt gtgcgcgctt
    16081 tcgccagata gaaatcttcc gacgactgca ccaccatcgg gcgcatctga ttagcacggt
    16141 tgcgggcatc tgccagacga ctttccggca acgggtgagt caataaaatt tccggcggac
    16201 gcgaggagta acgcgcctga tcgagtaatt tttcgaggaa ggttggcatc gcctgcggat
    16261 cgaatcccga gcgttgcagc acctgaatac caatgcggtc cgcttcctgt tcattttgct
    16321 gggtgaaact gatcatcccc tgacgcgttc ccgccagtgt accggtcagc gccgccatcc
    16381 ccgcctgcgg actggccatc gccagtaaaa tagaacctaa cgcgccgacc caggtcagcg
    16441 gcgcgctgcg ctgctgatct tccatcgctc gcgccaggtg acgttgggtg acgtgggaga
    16501 tttcgtgcgc cataactgaa gccagttgac tttcgttatc ggaataacgg aacagggcag
    16561 agtgcagcac cacgttgccg ccaaagaaag caaaggcgtt aatttcgtcg ttgttgatca
    16621 gaaagaaatg aaacggtgtc ttaaccgaat tggcatgcga aaccagacgc atccccagcg
    16681 aattaatata ttgcgttaac agcgggtcat taattaacgg cgcgctgccg cgtagctggc
    16741 ggacataata gtcgcccatc tgcatttcct gaccaatgga aagcgtgctt cctgcggagg
    16801 ttcccatatc cggcaaggtg tctgcgctgt cggcaaacgc cggggctacc tgaccaatgg
    16861 tcatagcagc aatgagggtt gcaaccaggt tttttttcaa ctgcctgaac ataacctcta
    16921 tcctgtattt ctgaagatag tcatttgacc gatactgtta aatatcgttc ccaccgcgcc
    16981 gcctttgagt gtaacggcga agattagccc actcaaatga cttttcagtt tcctgaatcc
    17041 gtcataaaaa gcgaagtctt ttttgtgaca tttcgataca attgcggatc gcaatcccgc
    17101 tcttgaggtt cagggaaggt ctttatgctc gaaatgttga tgcaatggta tcgccgccgt
    17161 tttagcgacc cggaagcgat tgccttgctg gttattttag ttgccggatt tggcattatc
    17221 tttttcttta gtggcctgct tgctccgttg ctggtggcta ttgtgctggc ctatttgctg
    17281 gaatggccaa ctgtgcgcct gcaatctatt ggctgctccc gccgctgggc gacgtcgatt
    17341 gtattggtgg tttttgtcgg tatattgcta ctgatggcgt tcgtggtact gcctatcgcc
    17401 tggcaacagg gcatctactt aattcgcgat atgccgggga tgctcaataa gctttctgac
    17461 tttgccgcca cgttgccgcg ccgctatccg gcgttaatgg acgcgggcat tattgatgca
    17521 atggccgaaa atatgcgcag tcggatgctg accatgggcg attcggtggt gaaaatttcc
    17581 ctcgcctcgc tggtcggttt gctgaccatt gccgtctatc tggtgctggt gccattgatg
    17641 gtcttcttcc tgctgaaaga caaagagcag atgctgaacg ccgttcgtcg ggtgctgccg
    17701 cgcaaccgtg gactggcagg acaggtgtgg aaggagatga atcaacaaat caccaactat
    17761 atccgcggca aagtgctgga gatgatcgtg gtggggatcg ccacctggct ggggttcttg
    17821 ctctttgggc tgaactattc gctgctgctg gcggtgctgg tcggcttctc ggttcttatt
    17881 ccgtacattg gcgcatttgt ggtgaccatt ccggtggttg gcgtggcgct attccagttt
    17941 ggcgcaggca cggaattctg gagctgcttc gcggtgtatc tgattattca ggcgctggac
    18001 ggcaacctgc tagtaccggt gttgttctcc gaagcggtta acctgcatcc gctggtgatt
    18061 attttatcgg tggtgatctt cggtggtttg tggggattct ggggcgtatt cttcgccatt
    18121 ccattggcga cgctgatcaa agccgtgatc cacgcctggc ccgatgggca aatcgcgcaa
    18181 gaataatctt cagcccgacg aactgtttcg ccgggctgat ttttcaggat tcctgacgta
    18241 aataaatagc ccgataacac atactcacca gcactgcacc gccgataata ttacccagca
    18301 tcaccgggag cagattggcg gtaataaaat ggctgaccgt cagtgccgga aaattgtctg
    18361 cgctactgtg cgccagttgc cagaaggagg taggggcgaa atggcgaatg gcaatggcga
    18421 acggaatcac aaacaaattg gcgatacagt gctcaaagcc actggcgaca aacagggtga
    18481 tgggcaaaat catggcgacg attttgtcgc ataacgaacg cccgcaataa ctcatccaca
    18541 gcgccaggca aaccatcaga ttgcacataa tgccgaggct gacagattca gtaaatgtat
    18601 gatgcatttt gccctcggcg cagtgtaaaa ccgcgactcc ccacatcgcg ttttcactca
    18661 tcaccagccc ggaaaaccag attaacaaac tgaaacaggc aatacctgcc agattaccgc
    18721 aggccaccag aagtgcgtta atcagccaag ttcgccaact aataacgccc cgactttttg
    18781 ccattaccgt cattaccgac gaggtgaaca ggttggtgcc gcaaaccgcc agcaaaataa
    18841 agccaagtgt aaagcaaagg ccgcccacca gatgggttaa tgcctgtgaa gacggggtat
    18901 cggcgataac ggaaaggtaa aaagtaaatc cgatcgccat aaacacgcca gcggacatcg
    18961 ccattaaaaa gaccgacact ttactgcggg caattttatg ggcggcaatc cgttcagcga
    19021 ttgccgcttt tctggcgctc aactgcaagt cgaagagagt ttgtttctca tgcatactcc
    19081 tgcaaaagca gattacaaca cctcgcgaac cgagattccc agacgctgca ttcgtgacag
    19141 cagcgtggtg cgcttcatcc ctaatcgtgt cgccgcgcca cggggcccgg caacaatgcc
    19201 attggtttct cgcaatacct gaacaatgcg ctgacgttct tcttcgtcat tctctggcgt
    19261 cgtcgggtgc agcaactgcg ccattgaact gcgaagcgct gaatcttcat ttagcgtcgg
    19321 cagtaaacgg ctttgtcgga cattaagatg taaattcaga ctgttaccgc gagtcagcag
    19381 taccgctcgc tcaatcacgt tttccagctc gcgcacgttg cccggccagt cccacgacat
    19441 caactggcgt agcgcctcgg tcgggatggc gtcaattgaa cgattcatat ggcgcgccat
    19501 tttttgcgtg aaatgctttg ctaaaagagg gatatcttcc ggacggtcgc gcagcggcgg
    19561 caattccagt gggaagacat tcaggcgata aaagagatcg ctgcgaaact ggcgatcttc
    19621 aaccatttgc cacaaatcac ggttggtggc ggcaatgact ctgacattta ccgggatcgt
    19681 tctactcccg ccgagacgct caatctcccg ttcctgcaat acgcgcagca gtttaggctg
    19741 aagttctaac ggcagatcgc caatttcatc gagaaacaac gtgccgccat cggcaatttc
    19801 aaaacgacca cgatgggtat taatcgcacc agtaaacgcc cctttgtcat gaccgaataa
    19861 ctcactttcc agaagactgg cggggatggc agcgcagttg attttgacca gcggcttgtc
    19921 gcgtcgcggg ctaagttgat ggatcgctct ggcgatcacc tctttgccgg ttccggtttc
    19981 accgcaaatc aacaccgtac tgtcgctctt cgccacaata tctacctgct ggagtaggtc
    20041 ttccattgcc tggctttgat agataatgtc accgatgccc agattcgaaa ggagctgctc
    20101 gctaagctgg tggttttctt gctgcaaact ttcctgcaaa tcggtcatgc tacgccaggc
    20161 atcggcattg ccaacggcaa tagcgatgcg atcggctatg tgttgtagta gctggcagtt
    20221 ttcctcacta aagagagtgg aagaggtatg cgccagcaac aatgcacccg gtgtatggtt
    20281 gccaaaggta agcggtataa ggagcgcaga ttcgcagccg ttagatgcaa gtaagagtaa
    20341 taacgggtcg cgttgccaga gaaacagatc gtctgcacgg tgcgtcaagg tcggttgtcg
    20401 ggtttgtagc gtttgtgtca gcaatacact gtcgccaggc atactgcgtg gcagacacgc
    20461 acaatgtgag gcagaaagat cgctgcacca caggctaaac ttctcgttct ttcgatgatc
    20521 gcccagtacc atactgactg aagccagacc gaaaaaatga tggatctcac gagcgacgtc
    20581 agcgatcaga tcgtcgagat caaaatgtga cagcactgca ttagtaatat ccaccagtac
    20641 ccgatgatga tcgcgatctt tctccagcaa acgcagcgcc acctgctgtt cttcatcatt
    20701 gagccatgat tgcagaaccg gggtgattat ccgcgccagc cagttaaaca gtgtgcaatc
    20761 ctggggagta aaacggctgc cgtgggtacg aataaagcgt agctccgcca ttccactact
    20821 gccgagaagg acggcgtgac gcgaataatc gaccatctcg ccctgatact ctgttgttgc
    20881 cgtcacatcg ctgccatcat catggtagcg gtaatagaag ttgcttttga gaacatgcag
    20941 ttcaataagt tgtaccgggc tgaacggcgg cggcatcggg atgaaggcgc gtaataaaga
    21001 cgccttaccg tgtttctgtg ctaaccgctc cgcgagcatt ccgtttaccg cttcaattgt
    21061 tattccttgt ggcggggcaa acatcgcctc gtctgacata gccatcaaca gtccttatta
    21121 atgagaaatc cttctcacgc aatttttctg gccatcagat agatggccgg ttcctgcaca
    21181 atttcatcca acagatgaat gaattgaatg ctacgcgcct gccacacaga cgtcgcgtca
    21241 tctgccagtg gcgcgaatgc ccgggtcaac agtgcggtgt ggctggcgtc aataacaatc
    21301 tcaccaaaag tcatcacccc cagcatctta cgtcgggctt gctcctcttc gaccagtgca
    21361 agccaatctt cgtattccgt cagtgggcag cggaaggcga cattcagaca atcaatcacg
    21421 ccaacgtgat gaccgatagc cagcgagtaa tacattacct gggagctgtg ttccggcatc
    21481 tcgtcgctac tggcgacaaa cttttttcgc agcgtccaga aaacaatttc cccgcactcc
    21541 tcagccatgt aacccccgga gaacgcgaaa gagttgctga acgatactat tgagacgcgg
    21601 atcgtcagct tcgcgcaacc aggtgttaac ccgatttcct gtaggatcgt cgcttaaatg
    21661 gcgcaggagc cgatcgcaaa tttctcgccc ctgacgatag cccgccagct gccgcgcttc
    21721 tcgctcaatg gcgatacgct gtgatggcgg gatctgcggc cacagcggtt gcatggtcac
    21781 cccggtggga tcgcgataat ccacagcgtg aatcttctgt tgcagcaaac cgagcgccac
    21841 ggcgaaaccg tgaatggtgg cggccggtgt tggcgggcag ccggggatcc aaacatcaat
    21901 ggggacaatg gtgtcgctgc cgccccagac gctgtagaga tcgtggaaaa tccgccgccg
    21961 gcaccgcacg cgccgtagga aacacagatt ttatgatcgg gggcagactc atacgcccga
    22021 agtgcaggca tacgcatcgc ccgggtgagt gcgccagtaa ataacaaaat atcggcgtga
    22081 cgcggtgatg aaacaacctt aatgccaaaa cgttctgcgt cgaatactgg tgtaatggcg
    22141 gcaaaaattt cgatttcaca ggcgttgcag ccaccgcaat cgacgcgata aacgtaggct
    22201 gagcgacgga tatcctgtag caaatgccgc ttcatctttt gcgtttgctc gtccagcgtg
    22261 atgggctggc tgacatattg cgtaagcact ggactcatag ctgctcctta gccaccagcg
    22321 gtacatctgt atcgtcgttg agcagcgtcg cgcgttgttt gcactccggg cagacgctcg
    22381 cttgcgccca taacatttcg cggttttgtg gcgcattttg ttgctgtgcc aacaattcag
    22441 cagccagtgc gacggttttt tgctgggcaa acgggcgttc gcaacggctg caacgttgta
    22501 gatggaacgt cgcgcgggta tagagatcgg ctttattggt gacggtcagt tcaaagttat
    22561 tggtaagctg atggctctgg tcgggcacac ttcttcacaa cgtccgcagt aaatacaacg
    22621 cctcagatag agctgccagg tgcgcgaatt ttgctggtcg tcggtctgga tagtcagcgc
    22681 atttgccgga caagcacagg cgccgcaggc aatacattgg ctgggcatca ggtccggttt
    22741 cccgcgaaag cctgggctga cctccagtgg cgcgaaggga tatttcaccg tcgcggttcc
    22801 ggcgcgcata atagttttca gtaacttcag catcttctgt ccttacttca gcggcgaacg
    22861 gttacgatca atgccgtagc gttcgatctc tttatacggc acggtttttg actggcgctt
    22921 gcgcacatct accagcgtca cacggtcggt acaggagtag cagggatcaa ggctaccgat
    22981 aatcagcggt gcgtcagaaa cggtattgcc gcgcagcatg taacgcaaca ccggccagtt
    23041 ggcgtaggtg gcggcacggc agcgccagcg gaacaatttt tggttatcac cgagcatgct
    23101 ccagtgcacg tcttcaccgc gtggcgcttc aacaaagccc agcgcgaatg cgtgaggttt
    23161 atagctaaag ccttcggtca gcagtggggt atccggcatg ttgtcgagag caaattctag
    23221 cattgccagc gaatcaaacg tctctttgac acggaccatc acgcgggaga aaacatcgcc
    23281 gccggtaaag gtaaacagtg tttttggaat attgccgtag tcggcgtagg ggtgatcaaa
    23341 gcgcaaatca cgggcgattt gtcggtcgag aatgccaatg ccctgagtgc gttgttccat
    23401 attcggcgta gcaagcagca tctctaccag ctccgacacg tcggcgcgca tctcgcgcac
    23461 cagtttcagc gtttgcagac gttgctcttt gagaatatcg cggcgaacac caccaatcag
    23521 attcagaccg taggttttac gcgacccgat cagcaattcc gccatcgtca tcgacttttc
    23581 ccgcacgcgg aaaaattgca taaagccggt atcaaaacca acgaaatggc aggagaggcc
    23641 aaggttaagc aaatgactgt gtagccgttc gacttccagc agaatcgagc gaatagtatg
    23701 tgctcgttgc ggcacctcaa tccccagtgc attttcaacc gagttggtat aggccacact
    23761 gtgggcaaaa ccgcaaatcc cacacacgcg gtccgataag aaggtcactt cgttgtagcc
    23821 catccgcgtt tctgccagtt tctccatgcc gcgatggaca taaaacaggc ggtaatcagc
    23881 atcgacaatt tgctcgccat ccacaaacaa gcggaagtga cccagttcat cggaggtgat
    23941 atgcagcggg ccgacaggga tcacctgcgc atcgctgttg ccctcattga taaacggata
    24001 cgtttcggaa tcagtcgtcg gttcagggcg cagtcgataa tccatcgcat ctttgcgcag
    24061 cggatgcata tcttccggcc agtcatcggg caacaccagg cgacgctgat ccggcaggcc
    24121 aaccggaatc agcccgtaca tatcgcgaat ttctcgctcg ccccagaccg cggcagggac
    24181 gcgcggtgtg actgacggaa actcccgact gtcggcatcg accagtgcct tcaccacgat
    24241 ccagcatttt tcggcccctt ccattgaaag ggcataataa accgcgtaat gaccgttaag
    24301 tgtccgctcg tcgttgccaa acaggactgg aagccagcca tcatgttgat aataaagata
    24361 atgtacaacg tcaggcagca gattgatttt caccgtaatg gtgacctgtt caggcgtttg
    24421 tcgctcttca tccagcaccg cgccggggaa ctgcgttttc agggcggcga gaatcgcttc
    24481 gccacgattt gacgatgaat taacgttcac tgtgtttctc cgaaacagat gcggtgcctg
    24541 aaggcaaaaa atcatgccag gtgctacgtt gtgcaggcag gtcgtgcgtc cctgagagga
    24601 ctatagtgga agcgcccgcc aggatcctga tgacaggttg tggaatatgc gttcccatcg
    24661 ccagcatcat gaccagcaga atcaccattg gcgaggtggt caaccagccg agatcacccc
    24721 ggttaacggc ctgcggcggt ttcgccatta acacccgcgc tgccatccgt accaggcccg
    24781 ccagcaccag cgttaacagc aataacagca ggacgataat cagcaggtga ttacgtgcca
    24841 gtccggcggt aatggtcata aattcgctaa gaaaaatgtt gaagggcggc atccctgcca
    24901 gcgccagcgc cagcgcaccg ccgccaaaca gcacggcggt aaatggcatg attttgagca
    24961 tcccacagac gacgttgaga tcgcgcgtgc cgtacttgag cagtacattg ccggaaccgc
    25021 agaacagcag cgtttttgcc agactgtggt ttaaggtgtg cagcagcgcg gcaaaaattc
    25081 ccagtggccc gccaatgcct agcgccaccg cgaccagtcc catgttctcc acgctggagt
    25141 acgccagcag acgcttaatg tcccgctgta ccagaatgaa aaatgccgcc acggcaaccg
    25201 acaacatgcc gaagatgagc aacaaccggt tggggaaatc gctgccgatg gcctggcaga
    25261 taatgatgta atagcgaatt agcaccaaca gcgcgcagtt cagcaatacg gcggagagca
    25321 gggcgctgac cggactcggt gcttcactgt gagcatccgg cagccaggcg tgcatgggaa
    25381 atagcccggt tttggtgcca aagccaatta gcacaaacac aaaggccaac agcattaatg
    25441 ttgggtcaag caaggacgat tgcttaagaa cctcgctcca gaatatcgcc atttctgcct
    25501 gcggcataac gctggcgacg ttggcgtata ccagcacggt accgaacaga ccaaaagcga
    25561 caccaacagt acaaataatg atgtacttcc atgctgcttc cagcgatgaa cgctgaccgt
    25621 aaatgcctac cagaaacgcc gagcttaagg tggtggcttc gatcgccgcc cacatcacaa
    25681 tcaggttatt gctggtaaca accagcagca tggtaaacaa aaacagatgg aagaaaccgt
    25741 agtaatcgca cagcgtaacg ggggaaagct cgccgtgtgc cacttcatga cgcatgtagc
    25801 caatcgagta aacaccggtg agaaagccaa tcacaccaag aatggcgagg aacaaaccgc
    25861 ccagaccatc aatatgaagc cacagtcccg cagcgaatat ttctcctgca tcagcggcag
    25921 tttggaccac ccagagtgcc agaatcagca gcagtgtgat ccctaagcta tgtaatacag
    25981 tcaccgtaca agtcgcagag agcccccgtt tccggcaggc aaaacagagc agcgaaaaaa
    26041 gcagcggcgt gagcaggagt aaagcgaaca tcacagaata actcatctca ttatcccttc
    26101 agcgcggtca agttgttcac gtccagcgtg ccgtgggtac gccatatttt tcttgccagt
    26161 aacaccatca caatgacggc gaagatggcg tcggtagcga tacctatttc caccagttcc
    26221 ggtgctcgcc aggcaagaag cgccagcacc agatgggagc cgttttccat caggcagtaa
    26281 ccaaaaattt gccgcaggat attgcgctgg ctgacaatgc acagcaggcc aagcagaaaa
    26341 tgacctaacg ctaccgccag cgccggtttc agcccggtag ccatcggtag cttcacgggc
    26401 tgaacgacaa atgcgcaaag caggacaatt aacgccgcga gcagtgccat cattgccgga
    26461 ccgaataacg ctttttccgg gatgttctgg ggaatatttc gtgcagcgta agtcatgatt
    26521 aacggtacca gcagcacttt ggtgataaag gcgctggcgg accagatcag cagttgctct
    26581 gcggcgaaca ggcacgagag agtggcgaaa atagacacca gcaccagcga ctggcaggcg
    26641 taaaatccgc atgacaggcg atagcttttg acgctaatca caaacagcga tgtcagcatc
    26701 atcagtcccg ccagattatt tacgatcata gaaccggtca ttcatcatct ccttactgca
    26761 accaggtcgc ggcgattacg ctggaaatca gcaacatcac aatcagtaca atcaacacca
    26821 ggcgcattga ccctggcagc ggtgcggcat cggcgacggc ctcgctcggt ttgccaggca
    26881 caacgcgacc aaaccagcga ataaaccagg cgaaactggc gaccgattca atcatcagca
    26941 gaatcatggc gggcagcagg atccagtact ccactgacaa cgcaaaaccg gcagcaaaca
    27001 gcgggaattt actgaagaag ccgttgaacg gcggcacgcc ggtaatcgcc agcgctgcca
    27061 cgcagaaacc cacgcctggc aacggcaggg tgtgcaatac gccacgcaga cgcggcaaca
    27121 agcgcgtgcc gcagctgtaa ctcagcgcac ctgctacaag gaaaaacagg cttttagcga
    27181 acgcgtggtt gacgatgtag gcgatgctgc cctccagtgc cagccgccca ctttcaccat
    27241 cgatgcggcg tggagatagg cgctgatcgg tgttggcgct tccattgcat ccggtagcca
    27301 cgcttgcatc ggtagctggg ccgatttccc ccacgcggca aacaggatgc cgccataaac
    27361 cagataacgt gcgtcgccat gtaactcgct catcgcgcta agcacaaacg ttccggtctg
    27421 caaaaacagc gtggcggcgg caagatacaa ccccaacgaa ccgatatggg tgataagcaa
    27481 cgcttttagt gctgaacgct gcgctttatc gctctggtaa taactgatca acgcccagga
    27541 gcagccgccc gtaatttcaa aaaacaacaa ctgaccgagc agcgtcgagg agagtaccag
    27601 tcccgccatc gcgccgataa acaccagcaa aaatgcgtaa taacgattcg tgccgttatg
    27661 cgggtgttcg cgatttttat ccgtcagata acccgtggag tagatcgtga ccagcaaacc
    27721 gagaaacacc accacaaaca gaatcagcgt actcacgcga tcaatgacca ggccaaacaa
    27781 tgccacatcc cccacgttga ccaacgtcaa cgtgacggca actttatcgg cctgataaaa
    27841 cgcggagatc agcgacaaca tgcacagcgt ggtcagtgcg gcgaacaaaa cccccattcg
    27901 gcggcccgac gttgtggcga aaacgaaacg accagtgcgc cgataaaagg cagcattaac
    27961 gtcgtcagag caagattttc catattccgt cagtgctcct tacagaccgg ttaaccagaa
    28021 gacccatgca agcgcagcaa ggctgaagcc aagccaggtc acatggtgaa tgagtaaaaa
    28081 acgcccgcgt gccagcgtgt tttctgcgat tgaggccagt acaaaaatca gcaaaacctt
    28141 gagcagcgta acgacaagtg aagtcagcag gcaggcgaga gaaagttctt gcgcgcgccc
    28201 aaagggcaga aacagggcca caaacagtgc tgccatcacg acctgtttca gccccagccc
    28261 ccatttcgct agcgccagcc cggcaccgga atattcggtc agcgggcctt cctgtaattc
    28321 ctgttctgct tcagcaacat caaagggaat ttttcccatc tcaatgaagc aggcaaaacc
    28381 acaggccagt aacgccagta cggtggttag cggcgagttc cagcccgttg ccagcgtatt
    28441 gctgatcatc tcgatatgcg tggaacctgc tatcagcgcc aataccagca gtgagagaat
    28501 gagcatgggt tcgaccagaa tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa
    28561 cgggcttccg gtatccagcc cggaaagagc aaagaaaaaa cgaaacaggg caagaagata
    28621 gataagggtg atcagatcgc cgccgcccgc aaaaggggaa acggtaataa aaagtggtag
    28681 ggccatcgcc agcaccagca tgctgctgat taatacccac ggcatcaggc ggaacatcag
    28741 acctgaagat ataggcgcaa cttcctggcg tttaaacagt ttgtggatat cgcgataatc
    28801 ctgccagatc cccggcccgc ggcgggagtg catacgcgcg cgtatctgcc gggaaatacc
    28861 cgtaaaaagc ggggttagca tcagcagaat cacggcctgt gctaacgcaa aaatgaccag
    28921 atatccgtcg taaagagttt gtctcatggt gatttcctta gacggcaata gcgattagca
    28981 gcacaaccag tgcggcgacc acatacaggc aatagagacg aaagtcgccg ctttgcagat
    29041 gctgtatttc tttggccagc cgttgggtgg cgctcacgat ggggcggatc acccgctcat
    29101 cccagaaggg ttctgtgctc tgagccctgg cggtgacgtg cgcaagacct ttatttagcc
    29161 tcagcgtagg gtcgaactgt tgtcgtagac gaaatagcgc agaaaagacc acacgcagcg
    29221 actgcatcac gccattgcct gacggggcca tcgcattttc ccagccgtag ccgcatgccc
    29281 aggcatctcc tgtgcgacga aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc
    29341 caggcagcaa aggcatcgcc agtagtaaca gcaacagtaa tgagggggtg acctgtgtat
    29401 gaaacgtgcc gggtacaagt gctattccgc tggcgacagt gacgggaggg gtattggtaa
    29461 acgcatgggc aatatgcatt atcttcggtg ccagccaact ggcactaagc gcaattaata
    29521 cgcagagtgc cgcgagcagt agcatggcga cgatcatcgt atttggcact tcctgtgcct
    29581 cttcagcgtg tgtactgcgc ggcgcaccac agaaagtaat accgtacatt tttacgaagc
    29641 acattactgc cagcccacca gtgactgcca gcattaccat agcaatagga cccgcaagtt
    29701 gtagcgctac ggcttccaca cgacttagtg agaacagcga ctgccaggtg taccattcgc
    29761 tgataaaacc attcagcgga ggaatggctg atatcgcgag gcaaccaatc aggcatgctg
    29821 cggctgtcca cggcatccgt ttcgccagtg cccccatttt ttccatgtcg tgggtatgca
    29881 aacgcgaaat aatcgcgccc gcgccgagaa atagcagccc tttgaacagc gcatggttga
    29941 gcagatgaaa cagtgcgccg agcagtccaa ccacggtgag cagcgggtcg tgcagtgaca
    30001 gaccgaccat cgccacaccg actgccagca aaataatgcc gacgttttcg acggtactcc
    30061 aggccagcag ccgtttgata tcctgttccg ccagcgcata cagcacgccc aggagcgcgg
    30121 agattgcgcc gatcgccatc accagaatgc cccaccacag cggcaaaccc gtttgcgcca
    30181 gcaggtccat cgctactttc aggatgccga aaataccgat tttgaccatt acgccagaca
    30241 tcaacgccga agcgtgcgat ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg
    30301 gcatcatccc ggctttcgcg ccaaaaccga aaaaggccag caggaacacc gccgacgcca
    30361 gccccggaga aagtgaaagc gtgcggaaac tggcaaaatc gaggctgccg ctttcgcgcc
    30421 ccatcagcaa gaaggcgatc ataatcagca ccgatccggc gtgggcgata aaaaagtaga
    30481 gcatgccagc gttgatcgac gttttatcct gcctggcaat gaccagaaac caggaagaca
    30541 gcgacatcat ttcaaacagc acgatgaacc aaaaagcgtt gtccatcacc agcagggcaa
    30601 ccatcgatgc gatgaaaaga ttcataaaga agcccatcgc cgccgcgcct ttgccctcgt
    30661 attcgcgcat ataagtcaat gaatagagcg aacaaaccac caccagcaag gagataacca
    30721 gcaccataaa tgccgagagg ctatccattc gcagggtaaa atcggcaaag tcaaaagggg
    30781 tggcaaaacg ggcgactaaa gttacaccag catgtaattg cgtaattccg ctgatgatgc
    30841 caatcacgcc gcccaccagc gatgtgatgc cggaaagctt aatagccagt ctgtccagac
    30901 cgagtaaaaa cagcgaagcc agactggcaa acagatagag gatcagcgac caggttaata
    30961 attgcagggc atccattagc gttgctcctg agtgagggaa aggggatcct ccccgccatt
    31021 ggcaaaccag cttgcggcaa ggcgctgttt ttctttcatc tgtcgttgca ggctatcgtc
    31081 ggtgatcagc cgtaacgcct gattcgggca agcgcgaacg caggccggac cttctggcaa
    31141 gaaatcacac aggtcgcatt tcaccgcgac ggtctggaca cccggttccc agcgcagcaa
    31201 tgcatgttgg ggcggcacgt tttcttcgcc gtctttgagt gagccttcag cctgaaaaac
    31261 atattgcgca ggggcattca ccggacggct tcctgaagcg ctgattgcgc caaatgggca
    31321 gaccacggcg caaagcttgc agccaataca gaggctttcg ttgagttgga tcgcatcatc
    31381 cctctgggag atggcattaa ccgggcagac ctgcaggcaa ggggcttcct cacagtgatg
    31441 acacacgaca ggggcagtga gtgttgacgt cttcgccagg gccaggcgcg ggtgttgctg
    31501 taaaccttgc gttttatgca cgtccgaaca ggcagcgaga caggtattac atcctgtaca
    31561 ccacagtggt tcggccacca caaagcggtt catgtcggct ccttgaaaat gatatgaaac
    31621 attgcatttc atattgcata aaggatgcca ttctgattaa ctcaatatat tcaataagtt
    31681 atgtattgac tggattctga acggtacaaa aatgatggtc attaacgttc agcaaaaaat
    31741 ggattcgaca cccgtgtcgc aggggaaagc gagaatgtga tcttgatcac gagaagtgag
    31801 gtatgctggc cgcaagcgca gccagcacgg aatggagcaa agtaatcagg cgtgttcttt
    31861 cagccagttc agcacaacgt cgtggtgatt gctggttttg aaatcgtcaa agacatgttc
    31921 gattttgcca tcagcgtcaa tcaggaagct gatgcgatga atgccatcgt aggttttgcc
    31981 catgaaggac ttttcacccc agacgccgaa ttgttcgcac acctggtggt cctcatcaga
    32041 taggagcgta aagttaagca gctctttttc cgcaaaacgg gagagttttt cgggtttatc
    32101 ggtgctgata cccagcacat caacgcccgc ttttttcaac tcatccatgt tatcgcgtaa
    32161 gccgcaggcc tgtacggtac agccgggggt catggctttc gggtagaaat aaaccagaac
    32221 acgctgtccc tggaagtcgg tcaaattaac ttgttctccg tcttgatccg gcaagctaaa
    32281 tttcggtgcg atatcaccgg ctttcagtgg attcattact taactccatc ctgttcatca
    32341 tgttgggaat aattgacgac gttaatactg ccttgtgcat tgagttctgt acatagggct
    32401 ttgaacgctt gctcaatatt tgctgcgtcc gcagatgcgg ggctgtgggc ggttatctga
    32461 atatgcaact gcgcagcccg ttcattttca gcaggttgcg tgcgcgacac cagctccgca
    32521 atgttcatat gatgcgcgtc gaaaagtgct gtgaagcgtt caattaaatg cggggagtct
    32581 gccacatcga cctgaaccca gacagatgct ggcattggcg gacgcggacg cgccgtcgtg
    32641 cgcttcatca cgattaaaag atccagttcg gcacctttca acggtaacgt tgattcaatc
    32701 agagtaatgg cattccatga accggaaagc agcataataa acgtgaactc ttctcccagc
    32761 atcgccaggc gactgtcttc aatattacag ccgcaactac tgacatgacg ggtgatggtg
    32821 ttcacaattc cagggcgatc ggcacccaac gcagtgatca ccagataatg ttgcgatgac
    32881 agtgtcaaac tggttattcc tttaaggggt gagttgttct taaggaaagc ataaaaaaaa
    32941 catgcataca acaatcagaa cggttctgtc tgcttgcttt taatgccata ccaaacgtac
    33001 cattgagaca cttgtttgca cagaggatgg cccatgttca cgggaagtat tgtcgcgatt
    33061 gttactccga tggatgaaaa aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat
    33121 tatcatgtcg ccagcggtac ttcggcgatc gtttctgttg gcaccactgg cgagtccgct
    33181 accttaaatc atgacgaaca tgctgatgtg gtgatgatga cgctggagct ggctgatggg
    33241 cgcattccgg taattgccgg gaccggcgct aacgctactg cggaagccat tagcctgacg
    33301 cagcgcttca atgacagtgg tatcgtcggc tgcctgacgg taacccctta ttacaatcgt
    33361 ccgtcgcaag aaggtttgta tcagcatttc aaagccatcg ctgagcatac tgacctgccg
    33421 caaattctgt ataatgtgcc gtcccgtact ggctgcgatc tgctcccgga aacggtgggc
    33481 cgtctggcga aagtaaaaaa tattatcgga atcaaagagg caacagggaa cttaacgcgt
    33541 gtaaaccaga tcaaagagct ggtttcagat gattttgttc tgctgagcgg cgatgatgcg
    33601 agcgcgctgg acttcatgca attgggcggt catggggtta tttccgttac ggctaacgtc
    33661 gcagcgcgtg atatggccca gatgtgcaaa ctggcagcag aagggcattt tgccgaggca
    33721 cgcgttatta atcagcgtct gatgccatta cacaacaaac tatttgtcga acccaatcca
    33781 atcccggtga aatgggcatg taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg
    33841 ccaatgacac caatcaccga cagtggtcgt gagacggtca gagcggcgct taagcatgcc
    33901 ggtttgctgt aaagtttagg gagatttgat ggcttactct gttcaaaagt cgcgcctggc
    33961 aaaggttgcg ggtgtttcgc ttgttttatt actcgctgcc tgtagttctg actcacgcta
    34021 taagcgtcag gtcagtggtg atgaagccta cctggaagcg gcaccgcttg cggagcttca
    34081 tgccccggct ggaatgattt tgccggtgac ctccggtgat tatgcaatcc cggtgaccaa
    34141 cggtagtggt gctgtcggta aggcgctgga cattcgtcca ccagcccagc cgctggcact
    34201 ggtttctggc gcgcgtaccc agttcacggg cgataccgct tcattgctgg tggaaaatgg
    34261 tcgtggcaat actctgtggc cgcaggtggt tagcgtgctg caggcgaaaa actacaccat
    34321 cacccaacgt gatgatgctg gtcagacact gaccaccgat tgggtacaat ggaaccgtct
    34381 ggacgaagac gagcagtatc gtggtcgtta tcaaatctct gttaagccgc agggttatca
    34441 gcaggcggtt acggttaaac tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc
    34501 ggcttccatg cagcgttaca gcacggagat gatgaacgtt atttccgccg gtctggataa
    34561 atctgccact gacgccgcga acgctgcgca aaatcgtgcc tccaccacta tggacgtaca
    34621 aagtgcagct gatgacaccg gtttaccaat gctggtcgta cgcgggccgt tcaatgtggt
    34681 ttggcaacgt ctgccagcgg cgctggaaaa agtgggcatg aaagtgaccg acagcacccg
    34741 ttcgcagggc aacatggccg taacttataa gccgctgtct gacagcgact ggcaggaact
    34801 gggcgcgagc gatccaggcc tggcatccgg tgactataaa ctgcaggttg gcgatttaga
    34861 taaccgcagc agcctacagt tcatcgatcc gaaaggtcat actctgactc agagtcagaa
    34921 cgacgcgctg gtagctgtct tccaggctgc gtttagcaag taaaaataca gggctggaat
    34981 catccggccc ttttttctga tatgatacgc aaacgtgtgc gtcggcagga aaacgcgatt
    35041 ttagcggtaa ttcgcacgaa atttgtttgt cggacgtagt tcggataagg cgttcacgcc
    35101 gcatccgaca aaacatccgg cacaccagac agcaaaagat tttaaaacgt taattcacac
    35161 ccaggagtga taaagatgca aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta
    35221 tacagcacgg aaaacccgga cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg
    35281 gatggcgcgc gcattgagca gtttgatcgc aaaggtatgg tgaacaacaa gttcaattac
    35341 ttcattatga gcaaactggc tgaagcgggt atcccgactc aaatggagcg tctgctctcc
    35401 gataccgaat gtctggtgaa aaagctggat atggtgccgg ttgagtgtgt cgtgcgtaac
    35461 cgtgctgctg gctctctggt gaaacgtctt ggaatcgaag aaggtattga gctgaacccg
    35521 ccgctgttcg atctgttcct gaaaaacgac gccatgcacg atccgatggt caacgaatct
    35581 tactgcgaaa cctttggctg ggtgagcaaa gagaacctgg cgcgtatgaa agagctgacc
    35641 tacaaagcga acgacgtgct gaaaaaactg ttcgatgatg ctggtctgat tctggtcgac
    35701 ttcaagctgg agtttggcct gtacaaaggt gaagtggtac tgggcgatga gttctccccg
    35761 gacggcagcc gcctgtggga caaagaaaca ctggagaaaa tggacaaaga ccgtttccgc
    35821 cagagcctcg gtggcctgat cgaagcctat gaagccgtcg cccgccgcct gggtgtacag
    35881 ctggactgat ttttctggtt gcttgtttct ttagccggag ggtactccgg ctaaatgtct
    35941 ttgatacccc cgctacaata cttctcatta tctccctttg acaggaaagt cataccaggc
    36001 caatatgtat atacataacg tatatcccaa aaaggggggg ctatggaaac tactgtattt
    36061 ttaagtaatc gcagtcaggc cgtcagatta ccgaaagcgg tcgctctgcc agaagacgtt
    36121 aagaaagtag aaattattgc gattggtcgt acaagaatca tcactcccgc cggtgagtcc
    36181 tgggacagtt ggtttgatgg cgaaaatgtg agtgcggatt ttatggatat ccgtgatcaa
    36241 cccgcaatgc aggaaaggga atcattttga tgctgaaatt catgctggat accaatatct
    36301 gtattttcac tattaagaat aaaccagtac atgtcagaga gcggtttaac ctgaattctg
    36361 gccggatgtg tataagttct gtaacattga tggagcttat ctatggggca gaaaaaagtc
    36421 agatgccaga acgcaatttg gcggttattg aaggtttcgt atctcgtttg gtagtgcttg
    36481 attatgatag tgctgctgcg acgcataccg ggcaaatcag ggccgagctt gcccggcagg
    36541 gaaggcctgt tggacctttt gaccagatga tagcgggaca tgctcgcagt cgcggtttga
    36601 ttgtggttac caacaatacc cgagagtttg agcgagtggc gggaatcaga ctagaagact
    36661 ggagctaaca ttctctttta cttctaacat tcgtaaatta tataacgtgg catgaagcct
    36721 taattcctcc gccatttcat cccgctttcc tgtggtaatc ttcctctgaa ccacctacga
    36781 tcatttctat aatgaatata ttgttgaggt atctatgcgt tggcaaggac gacgtgaaag
    36841 tgacaatgtt gaagacaggc gcaacagctc cggtggtccg tctatgggcg gtcccggttt
    36901 tcgcctgcca agcggtaaag gcgggctgat tttactgtta gtcgtgctgg ttgcaggcta
    36961 ctatggtgtt gatttaaccg ggttaatgac tggacagccg gtttcccaac aacaatcaac
    37021 gcggtccatt agccctaatg aagacgaagc tgcaaaattc acctcggtga ttctggcaac
    37081 cacggaagac acctggggac aacagttcga gaagatgggt aagacctatc agcaaccgaa
    37141 gctggtcatg taccgtggaa tgacgcgcac cggctgcggg gctggtcagt ccattatggg
    37201 gccgttctat tgcccggcgg atggcacagt ttatatcgat ctctccttct atgatgacat
    37261 gaaagacaaa cttggcgcgg atggcgattt tgcccagggg tacgttatcg cccatgaagt
    37321 cggtcatcat gtgcagaaac tgttaggcat cgagccgaaa gttcgtcaac tgcaacaaaa
    37381 cgcgacgcag gcggaagtga accgcttatc tgtgcgtatg gaactccagg ccgactgttt
    37441 tgccggtgtc tgggggcata gtatgcagca gcaaggcgtt ctggaaaccg gcgatctgga
    37501 agaggcgctg aacgcggcgc aggccatcgg cgatgaccgt ttacaacagc aaagtcaggg
    37561 gcgagtagta ccagatagtt tcacacatgg cacttctcag caacgctaca gctggtttaa
    37621 acgtggtttc gacagcggcg atccggcaca atgcaatact tttggtaaaa gcatttaact
    37681 tccggggcag ggatggctga actgactgcg cttcacacat taacagcgca aatgaaacgt
    37741 gaagggatcc gccgcttgct ggtgttgagc ggggaagagg gttggtgttt tgatcatgcg
    37801 cttaagttgc gtgatgcctt acctggcgac tggctgtgga tttcgccgca gccagatgct
    37861 gaaaaccact gttcaccctc ggcgctacaa actttacttg ggcgcgagtt ccggcatgcg
    37921 gtattcgacg cccgccacgg ctttgatgcc gctgcctttg cagcacttag cggaacgttg
    37981 aaagcgggaa gctggctggt tttgttactc cctgtatggg aagagtggga aaaccaacct
    38041 gatgccgatt cgctgcgctg gagtgattgc cctgacccta ttgcgacgcc gcattttgtc
    38101 cagcatctca aacgcgtact tacggcggat aacgatgcta tcctctggcg gcaaaaccag
    38161 ccgttctcgt tggcgcattt tactccccgt actgactggc accccgcgac cggcgcacca
    38221 caaccagaac aacagcaact cttacagcag ctactgacca tgcctccggg cgtggcagcg
    38281 gtaacggctg cgcgtgggcg cggtaagtcg gcgttggcag ggcaactcat ttctcgtatt
    38341 gcgggcagtg cgattgtcac cgcgcccgca aaagcggcaa cggatgtact ggcacaattt
    38401 gcgggcgaga agtttcgctt tattgcaccg gatgccttgt tagccagcga tgagcaagcc
    38461 gactggctgg tggtcgacga agccgcagcc atacctgcgc cgttgttgca tcaactggta
    38521 tcgcgttttc ctcgaacgtt gttaaccact acggtgcagg gctacgaagg caccggacgt
    38581 ggttttttgc tgaaattttg cgctcgcttt ccgcatttac accgttttga actgcaacag
    38641 ccgatccgct gggcacaggg atgcccgctg gaaaaaatgg ttagtgaggc actggttttt
    38701 gacgatgaaa acttcaccca tacaccacaa ggcaatatcg tcatttccgc atttgaacaa
    38761 acgttatggc gaagcgagcc agaaacgccg ttaaaggttt atcagttatt gtctggtgcg
    38821 cactaccgga cttcgccgct ggatttacgc cggatgatgg atgcaccagg gcaacatttt
    38881 ttacaggcgg ctggcgaaaa cgagattgcc ggagcgctgt ggctggtgga tgagggggga
    38941 ttatctcaac aactcagtca ggcggtatgg gcaggttttc gtcgcccgcg gggtaatctg
    39001 gtggcccagt cgctggcggc gcacggtagc aatccactgg cagcgacatt gcgtggacgg
    39061 cgggtcagcc ggatagcagt tcatccggcg cgtcagcggg aaggcacagg gcagcaactt
    39121 attgctggtg ctttgcaata tacgcgtgac ctcgactatc tttcggtgag ttttggttac
    39181 accggggagt tatggcgttt ctggcaccgc tgcggttttg tgctggtgcg gatgggtaat
    39241 catcgtgaag ccagcagcgg ttgctatacg gcgatggcgc tgttaccgat gagtaatgcg
    39301 ggtaaacaac tggctgaacg tgagcattac cgtttacgtc gcgatgcgca agctctcgca
    39361 aagtggaatg gcgaaacgct tcctgttgat ccactaaacg atgccgtcct ttctgacgac
    39421 gactggcttg aactggccgg ttttgctttc gctcatcgtc cgctattaac atcgttaggt
    39481 tgcttattgc gtctgctaca aaccagtgaa ctggcattac cggcgctgcg tgggcgttta
    39541 cagaaaaacg tcagcgacgc gcagttatgt accacactta aactttcagg ccgcaagatg
    39601 ttactggtcc gtcagcggga agaggccgcg caggcgctgt tcgcacttaa tgatgttcgc
    39661 actgagcgtc tgcgcgatcg cataacgcaa tggcaatttt ttcactgact ccttcaattt
    39721 tctcccatgg tcattgtgag tcagcggcgt aaaattaatg acatcagtta aggagttcgc
    39781 catgaaacat gaccattttg ttgttcaaag cccggataaa cctgcgcaac agttgttact
    39841 gcttttccac ggtgtcgggg ataacccggt ggcaatgggg gaaataggta gctggtttgc
    39901 accgctgttt cctgatgcgc tggtggtgag tgtcggtggc gcggagccga gcggtaatcc
    39961 ggcggggcgt cagtggtttt cagtgcaggg tattacggaa gataatcgcc aggcgcgtgt
    40021 ggatgccatc atgccgacgt ttattgaaac ggtacgctac tggcagaaac agagcggagt
    40081 aggggctaac gccacggcgc taattggttt ctcacaaggc gcgattatgg cgctggagag
    40141 cattaaagcc gaaccgggcc ttgcttcgcg cgtcattgcc tttaacggac gctatgcgag
    40201 cctgccggag accgcgtcta cagccacgac aattcacttg attcatggtg gtgaagatcc
    40261 ggttatcgat ctggcgcatg cagttgcggc gcaggaagcg ttaatcagtg ccggtggtga
    40321 cgtgacgctg gatatcgtgg aggatttagg ccatgcgatc gacaatcgca gtatgcaatt
    40381 tgccctggat cacttgcgtt ataccattcc gaaacattat ttcgacgaag cgctaagtgg
    40441 cggtaagcct ggcgatgatg atgtgattga aatgatgtaa aagacagatt acctggcctg
    40501 ataagacgcg tgagcgtcgc atcaggcatc gttcaccatt gccggatgtg gcgttaacgc
    40561 cttatccggc ctacggttga gcactcagct tacttcttcg gttgatcctt tttcggccag
    40621 tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg ggggaagctc cggcttgttg
    40681 gcgagaaatt tcttgtggtc gacgcgcttg agatctttaa tcacgttcag caggacgcct
    40741 accaaaaaga caatcaccag aatccaccaa tatttagcca gccagtccat gcttatttcc
    40801 tcttgcagaa ccactcatca ggcgacgagc tgttccatga tacgttgata catacgggca
    40861 agtagctgca ggtcggcagc gttcacacat tcattaattt tatgaatagt ggcattgacc
    40921 ggcccgagtt ccaccacctg cgcccccatg cgagcaataa agcgcccgtc ggacgttccg
    40981 cctgtggtca gtagctgcgg tttaatttca ttatagtgct caaccgcgtt aacgaccgca
    41041 tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg aaagccacca gtccaccgta
    41101 taacgcagtt gatgtttttc aagcagggca agcacctgtg ctttgatcat ctcatcagtc
    41161 agttcggtgc tgaagcggaa gttaaactgc acaaacagtt cacccggaat aacattgtta
    41221 ctgcccgttc ccgcctgaat attagcaatc tgcatactgg tcgccgggaa aaattcattg
    41281 ccctgatccc actcaatagc cactaattca ttaaggaaag gtgctgcgcg atgtactgga
    41341 ttgtcagcca gatgcgggta ggccacatgc ccctgaacgc catgaatggt aaggttgcag
    41401 gttaacgatc cgcgacgacc atttttcacc acatcaccaa ccacttcgat actcgacggt
    41461 tcgccgacca ggcagtaatc gagacgctca ttacgtgcca tcaacgcttc gacgactttt
    41521 accgtaccgt tgtgggcact ggcttcttca tcagaggtga tcagaaatgc cagtcgcccc
    41581 gtatggttgg gatgttgtgc gacaaagcgt tctgccgcta ccaccatcgc cgccagcgag
    41641 cctttcatat ctgccgcacc gcgtccgaat aacatgccat cacgaatggt gggttcaaac
    41701 ggcggattga tccaacgatc ggcgtcgcca ggcggcacca cgtcggtatg cccggcaaag
    41761 gctaacgttt caccttgccc acgccatgcc caaaaattct gcgtatcggc aaagtccatg
    41821 cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca acaaagcctg gcatcctgcg
    41881 tcatcaggac tcagggaagg gcggcgaata agctgttgtg tcagctcaat aaccgggcac
    41941 gacatagact acacctcatg gaaaaattgc tgataactgg aatcactgaa acccagcagc
    42001 ataggcttac caggcgcgca gagcaatgga cgtttgataa ttgcaggcat ttcagtcatt
    42061 aatgccgccg cagaggccgc atcggtgatt ttattgcggg tggtttcgtc cagtttacgc
    42121 caggttgtac cacgggtgtt cagtaacgct tcccagccta attcgttgat aaaaccgttc
    42181 aataattcgc tgtccagccc atcgacgcgg taatcatgaa aacgatagtc gatgttattg
    42241 gcttccagcc aacggcgagc ctttttaatg gtgtcacaat ttttgatgcc gtaaagtgta
    42301 accataatga atcctttttc gatttatgag aataaatact catttaaggg tgaaaacatt
    42361 ttgcctgcaa atgattatca ggaaatataa cattatcgaa gtattcaaat taacaactat
    42421 taaataatat taaacatttt taacgtaaac attaagaata ttcatcttgt tatctgtctg
    42481 tattttaacc agaattaaat cagtattaga tgccgtatga atatctcgaa atgttgctgt
    42541 gtatcacata caaattatca ccgcaggacc atagtattta cataaggttt gtcccccgga
    42601 ggtagtcatc gcagcaatcg agtaaattag cttcaccacg gtatattgtt tccgtagaat
    42661 acccataatt ataagagagg ttgttatgat tgaacgtgaa ctggggaact ggaaagactt
    42721 tatcgaagtt atgcttcgta agtaattccc gagacaaagt ataaacagca agaacccgct
    42781 gagcaggttc ttaatcggaa agttgaaaaa ggcgacacat tggcatgtcg ccttttttat
    42841 tgcttattcc gggcgcggct tcagcgggaa gcggcggcgc accagcacaa agaacagcgg
    42901 cacgaagtaa atagccagaa tagtagctga aatcatcccg cccattacgc cagtacccac
    42961 cgcatgctga ccaccggaac cggcacccgt gctggttgcc attggcaata cgccgaagat
    43021 aaatgccagc gaggtcatca gaatcgggcg taaacgctga cggcaggcgt ggagcgtcgc
    43081 ttcaaacagg tcgtggcctt tttggttcat ctcgttagca aactcgacga tcaggatggc
    43141 gtttttcgcc gataaaccaa tgaccgttaa caggcccacc tggaagtaaa cgtcgttttc
    43201 cagcccgcgc atccaggttg ccagcagcgc gccgataacc cccagcggca cgaccagcat
    43261 taccgagaac ggtaccgacc agctttcata caatgccgcc agacacagga atactaccag
    43321 caaggaaatg gcgtacagcg ccggagcctg cgcgccggaa agccgctcct gatacgacat
    43381 cgccgtccac tccagaccaa agccgtttgg caactgcttc actaacgatt ccataatatc
    43441 catcgccgta ccggtactga cccccggtgc ggcttcccca acaatctcaa ccgcagaata
    43501 accgttatag cgttccagac gcggcgagcc tgtctcccag cgtgaggtcg cgaaagcaga
    43561 gaagggcacc atgccgccat ctttatttcg gacataccag agattaatgt catctggcag
    43621 catgcgatac ggcgcagctg cctgcacata gactttcttc acgcgaccgc gatccataaa
    43681 gtcattcaca tagctcgaac cccaggcggt ttgcagtgtg tcgttaatat cgtcgatagc
    43741 aacgcccagc gcctgagctt tacgctggtc gatatcaatc tgcaactgcg gactgtcatc
    43801 gaggccgtta tggcgcacac gggttagctc cgggttttcc gccgccagcg ccagcaactg
    43861 gttacgtgct gccatcagcg catcgtgacc tgctccagcg tggtcctgca actccatatc
    43921 aaaacctgca gaactaccaa gaccgctaat tgctggcggg ctgctggcga taacgcgagc
    43981 ttctttaatc ttgttaaacg ccttcgttgc acgctcgata atggcaaatg aggtgccggt
    44041 cttactgtcg cgttcgctcc agtctttcag gcggataaac attcgcgcca cgttttgccc
    44101 gttacccccg gggccagaac caacagtggc aaacaccgac atgatgttgt ctttttcatg
    44161 ggtgaagtaa tatttctcga tttgctcaac gactttcagg gtctgttgtt gggttgaacc
    44221 gctgggcaat tgtaccgagg tagtaaacat gccacggtct tccagcggta agaacgacgt
    44281 cggcaaacgc aggaacagga acaccatgcc gccaagcagc aggacataaa tcacaatcca
    44341 gcgcaggcta cggtggagaa ttttcgccac ccctttttcg tagcgttcgg cgttgcggtt
    44401 aaacatctgg ttaaaccagg caaaaaagcc tttttgcccg tgatgctcac cttttttcag
    44461 tggcttaagt agtgtggcac acagagccgg agtgaggatc atcgctacca gtactgacag
    44521 caccatcgcc gcaacaatgg taatagagaa ctggcgatag atggcaccgg tggtgccgcc
    44581 gaagaaggcc attggtacaa ataccgccga aagaaccatc gcaatcccga ccagtgcccc
    44641 ctggatctgc cccatcgatt tacgtgtggc ttcgcgagga gtgagtcctt cctcactcat
    44701 aatacgttcg acgttttcca ccaccacgat tgcgtcatcc accagcagac cgatcgccag
    44761 caccatcgcg aacatggtta aggtgttgac gctgtaaccg aaggcgtaaa gtacggagaa
    44821 ggttcccatc aacaccaccg gcacggcgat agtgggtatc agcgtggcgc ggaagttttg
    44881 caggaacaga tacataacga ggaaaaccag agcgatagct tccagcagcg ttttcaccac
    44941 gtcttcaatg gaggctttaa caaacgaggt ggtttcatac gccaccttgt attccagtcc
    45001 gtgcgggaaa tactgcgcca gctcgtcgag acgattgagc accagctccg ctgtcgccat
    45061 ttcgtttgcg ccggaggcca gttttacccc cagcccggag gctggcttac cattgaagcg
    45121 gctaagataa tcgtatttct ccgcccccat ttcgacggtg gcgacatcgc ccagccttac
    45181 ctctgagccg tcttgattca ccctcaaggt gatatcgcgg aactgttctg gtgtttgcag
    45241 cagtgactgg gcgttaatgg tggcgttgag cgcctgctta tcgacggaag gtgtaccacc
    45301 aagttgccca accgcaatct gcgcgttctg tgactcaatg gcatcagtga catctttagc
    45361 cgtcatctgg aaactgttga gtttcgccgg gtccagccag atacgcatgg aatattgcga
    45421 accataggca tcgatatccc cgacgccatt cacgcggctt aacgggtcct gaatattact
    45481 ggcaacataa tcggcaatat cctgtttatc catcgaacca tcggtagaga cgaaagcaat
    45541 ggtcagaata ttggtatcgc cggttttacg caccgtcacg ccctgatttt gcaccgcctg
    45601 cggtaacttt cgcatggctg attgcagctg gttttgtact tgctgcacgg cttcgtccgg
    45661 atcggtgcct gctttaaaac ttaaagtgac agatgcctga ccggtgccac tgctctgaga
    45721 tgacatatac atgagattat cgaggccggt catattttgc tcgataacct gggtcacggt
    45781 gttttccagc gtctgggccg atgcgccggg atagttagcg gtcactcgca cattcggtgg
    45841 cgcgagatcg gggtattgtt caacgggcaa tgagaaaatc gccagggtac ctgtcagaca
    45901 caacaggatt gccagcaccc aggcaaaaat ggggcgatca ataaagaaat tcgccattaa
    45961 aagaggacct cgtgtttctg cgtatcgttg taggcttgac ttgcttcact gtagcggcaa
    46021 ggtacgagcc aatcgtggag aaaaaaagga gttaatgtaa attatcatgc ccttttatac
    46081 tgaaccttaa gtgcaagtat tctttggtca gtaggaggca ttacatcagc tgactttcct
    46141 caccctccgc ggagcgaaag ctaatactca ctaacgtgcc gccgccggaa ggttgtgaaa
    46201 aagtcagcgt cccacccagc cgttccgcgc gttcgcgcat gatattcaga ccataatgac
    46261 cttcgggctc tttcggttca ccgatgccaa tcccgttatc acggatataa accgtgtgat
    46321 tgccgtccgg cgcggtgacg caactgacgg cgatttcgct ggcgttggcg tgcttcatcg
    46381 cattcagcac cgcttcgcga ataatttgca acaaatgcac ctgcatttgc gcatccagcg
    46441 ccagggttgg cagacggcag tcgagggtca gtttggcgct ggtttgattt tgtaacgtat
    46501 ccagcatttc cctcaacgcg gaggggagat ccgcctgctg cagcgtcagg cggaaagtag
    46561 tcaacagctc gcgtaactgc cgataagcat cattcaacgc ctgggaaaaa tcggccatga
    46621 tactttgtgc ggtggcgtta tcttccggta tcgaacgctt cagtaacgtc aactggatac
    46681 gtaagtaaga aagtacctga gccagcgagt cgtgcaattc gcgggcgatg gttgcacgtt
    46741 cttccatcaa caataactgc tgaaaatgct tctgcgcctg attaaagtac aaaccgcgtc
    46801 ccagcatcga cgaaacgctg ttaagcagcg gttctgatga tgaaacgtga ctattttgcc
    46861 agtgcagttc gccgtaaacc gtctcttgca ttgtcaccgg taaaatctgc atcggcaatt
    46921 ccgggtttgg ttgcccttcg ctaatccgcc agttttcacc gacatttaac tccagatatt
    46981 cagccgcttc attgtcgcga acaatctgca aaatatggcg gaaacaatgc acatcaatct
    47041 ggctggtgtt cagcgcctgg gaacattgat acaacacctc cagacgacgc ttggcctcgt
    47101 ggagatcgcg ggtcttttct tctactgacg cctccagcga aaggtacaat ttatgcagct
    47161 cgctcgacat ctggttaaag gtttttgcaa gcagaccaag ctcattcggc agactggtat
    47221 ccagcggcgg cgagtcgaac tgcccgtgtt caatacgttg actggcggta accagctgat
    47281 tcagcggggc aaccacctga tggcgtatac ggcgcaaagt aaaaaagacc agcgtgaaaa
    47341 taccgatacc gccagccagg gaaatcgcca ccaccagcag cattttgcgt tcagcgtagt
    47401 gctgtaaagc cagcacgaac aggtctatct gattaacata attattaata ttggcctgat
    47461 accacggcaa atcgcccttg ctgagccgat tattcatctc cagccagttg gcattcagat
    47521 gcgcatagcg agtttttact gcttctggca cataccagac gttgagattg gttaataccg
    47581 gtgaatgcag tgcctgctga aacaactggc gatgtgcatt gagttgtgga ctgccacttt
    47641 gcaagtcgta gcccaggcgg taactctgca tacgtagcga tccggcaata ttgatagcct
    47701 cagcgtcgcg caaactgctc gccagagtta gcagagcgat acccgtggaa agaatcgaca
    47761 gcagcacaat gtaaaaaaag gcccgggcca gactggccga gacggggcgt ttaacaatca
    47821 cacgcgtctt ctccacaaaa aatcattaat gttccagtta gctgaaacac aatttagtca
    47881 gtgatgggat tatttgatct gccacagttt atagcgaaat agttacttat tctgggcaca
    47941 tgaacattgc cgattcccct gtaagtcggg taataacaac actcatataa agaataaggt
    48001 ttttacaacc aaaaaagaag gtcgttatga atcgttttat tatggccaac agtcagcaat
    48061 gtctgggttg tcatgcttgt gaaatcgcct gtgtcatggc tcacaatgat gagcaacatg
    48121 tcctgagcca acaccatttt catccccgaa ttacggttat caaacatcaa cagcaacgta
    48181 gtgcagtgac ctgtcaccat tgtgaagatg cgccctgcgc ccgtagctgc cctaatggcg
    48241 caatcagcca cgttgatgac agcattcagg tcaatcagca aaagtgtatt ggctgtaaat
    48301 cctgcgtggt ggcctgtcct tttggtacga tgcaaatcgt cctgacaccc gtcgcggcag
    48361 gaaaagtaaa agccacggcg cataaatgcg acctttgtgc ggggcgcgaa aacggtcctg
    48421 cctgtgttga gaattgcccg gcggacgcgc tgcaactggt cactgacgtc gcactctccg
    48481 gcatggcgaa atcccgccgc ttgcgcaccg cgcgtcagga acatcaaccg tggcatgcca
    48541 gtaccgcggc gcaagaaatg ccggtaatga gtaaagtcga acaaatgcag gcaacgcccg
    48601 cgcgtggcga gccggataaa ctggcgattg aagcgcgcaa aaccggtttt gatgaaattt
    48661 atctgccatt tcgcgccgac caggcacaac gggaagcctc gcgctgcctt aagtgcggcg
    48721 agcacagcgt ttgtgaatgg acctgcccgc tgcataacca tattccgcag tggattgaac
    48781 tggtgaaagc cggaaacatc gacgccgccg tcgagctttc tcaccagacc aacaccctgc
    48841 cggaaattac cggacgcgtt tgtccgcaag accgtttgtg tgaaggtgcc tgtactattc
    48901 gcgatgagca cggtgctgta actatcggca acattgaacg ctatatttca gatcaggcgt
    48961 tggcgaaagg ttggcgtcct gacttaagtc atgtcaccaa agtggacaag cgggtggcga
    49021 ttatcggggc aggcccggca gggctggcct gtgcggatgt tctgacccgc aatggcgtgg
    49081 cggtgacggt gtacgatcgc catccagaaa ttggtggctt gctcactttc ggcattcctt
    49141 ctttcaaact ggataaatcc ctgctggcac gccgtcggga aatcttcagc gcgatgggga
    49201 ttcacttcga actcaattgt gaagtgggta aagatgtctc tttggattcg cttttggaac
    49261 aatacgacgc ggtcttcgtt ggcgtaggca cttaccgttc catgaaagcg ggtttaccca
    49321 atgaagatgc gccgggcgtt tatgacgcgc tgccgttcct cattgccaac actaaacagg
    49381 tgatggggct cgaagagcta ccggaagagc cgtttatcaa taccgccgga cttaacgtcg
    49441 tggtactggg cggcggcgac accgcgatgg actgtgtgcg tactgcgctg cgccacggcg
    49501 cgagtaacgt cacctgcgct tatcgtcgtg atgaagctaa catgccaggc tcgaagaaag
    49561 aggtgaagaa cgcccgcgaa gagggggcca acttcgaatt taacgtccag ccggtggcgc
    49621 ttgagctgaa tgaacaaggt cacgtttgcg gaattcgttt cctgcgcacg cgtcttggcg
    49681 agccggatgc ccaggggcgt cggcgacctg taccgattga gggcagtgaa tttgtcatgc
    49741 cagccgatgc ggtgattatg gcgtttggct tcaatccaca cgggatgccg tggctggagt
    49801 cgcacggtgt aacggtagac aaatggggcc gcatcatcgc ggatgtggaa agccagtacc
    49861 gttaccagac caccaatccg aaaatcttcg ccggtggcga cgccgtgcgt ggtgcggatc
    49921 tggtggttac cgcaatggca gaaggacgtc atgcggcaca ggggattatt gactggctgg
    49981 gggtaaaatc agtcaaatct cactgatagc ctgcgcagac aaacccgact tcacagcgta
    50041 agataattgt tcatttcgcg ctgtggagtc ggtatgacgc aacaaatcac cctcattaaa
    50101 gacaaaattc tctccgataa ctatttcacc ctgcacaaca ttacctacga tctcacgcgc
    50161 aaagatggcg aagttatccg ccataaacgt gaagtttacg atcgcggtaa tggtgcgacg
    50221 atcctcctgt acaacgcgaa gaaaaagagc gtggttctga ttcgtcagtt ccgtgtcgct
    50281 acctgggtta atggcaatga aagcgggcag ctgattgaaa cctgcgccgg gctgctggat
    50341 aacgacgaac cggaagtatg cattcgtaaa gaagcgattg aagagacggg ctatgaagtg
    50401 ggcgaagtgc gcaaattatt tgaactgtat atgtcgcctg gcggtgtgac tgagctaatc
    50461 cactttttta tcgccgaata cagtgacaat cagcgcgcta acgccggtgg cggtgttgaa
    50521 gatgaagata ttgaagtgct cgagctgccg ttcagccagg cgctggagat gatcaaaacc
    50581 ggcgagatac gtgacggtaa gacggtgtta ttgcttaact atttgcaaac gtcacattta
    50641 atggactgaa aaataacaat aatatttcgt tgtttattat tggctcaatc cgacaaatct
    50701 gattgagccg cgctacctgc gcaacgaaga tacgacttgt gctgtttgtt tgaacttctg
    50761 gggtcgtacc gtccatgcgc tatcgcattt tccttctctt ttttttcgct ttgttgccga
    50821 cgtctttggt gtgggcggca ccagcgcaac gggcgttttc cgactggcag gtcacctgta
    50881 ataaccaaaa cttctgcgtg gcgcgcaata cgggcgatca taatggactg gtgatgaccc
    50941 tgagccgcag cgccggggcg cataccgatg ccgttttacg tattgagcgc ggcgggttga
    51001 agtcgccgga tgcgtctgaa ggagagatag cgccacggct gctgttagat ggtgagccgt
    51061 tggcgttaag tggtgacaag tggcggattt caccatggtt attagtaacc gatgatacgg
    51121 caaccatcac cgcgtttttg cagatgattc aggaagggaa ggcgatcacc ttacgcgacg
    51181 gcaatcagac catttctctg agtggcttaa aagcggcgtt gttgtttatt gatgctcagc
    51241 aaaagcgcgt tggcagtgaa accgcgtgga tcaagaaagg ggacgaaccg ccgctcagcg
    51301 taccgcccgc gcctgcgctg aaagaggtcg cggtggttaa cccaacgccg acgccactct
    51361 cactcgaaga acgcaacgat ttgctggatt atggaaactg gcggatgaat ggtctgcgct
    51421 gctcgcttga tccgttacgt cgtgaggtga atgtcactgc gctgactgat gataaagcgc
    51481 tgatgatgat tagctgtgag gcaggggcct ataacaccat tgatttggca tggattgtgt
    51541 cgcgtaaaaa gccactagcc tcgcgcccgg ttcggttgcg tttgccattc aacaacggtc
    51601 aggagacgaa tgaactggaa ctgatgaacg caacatttga tgagaaatcg cgtgaactgg
    51661 tgaccttagc gaaagggcgc ggattaagcg attgtggcat tcaggcgcgc tggcgctttg
    51721 acggtcagcg ttttcgcctg gtgcgttatg ccgcagaacc cacctgcgat aactggcatg
    51781 ggccagacgc ctggcccacg ttgtggatca cccgttaaac aaaattgcgg ggacatgtag
    51841 gccggataag acgctgtagg ccggataaga cgcgtcagcg tcgcatccgg caatcagcat
    51901 ccggcgatca ccatcaggca cctttcactc ccagcacctt atgcgctttt gccacaatat
    51961 tctcggcggt aaagccaaag aacgggaaca gcttatccgc cggagcagat tccccgtaac
    52021 ccgtcatccc gacaattgcc cctttcagac caacatactt gtaccagtaa tcggcaatac
    52081 ctgcttccac cgccacgcga gccgcaacgt tagaaggcaa caccgactcc cgatattcct
    52141 catcctgggc gtcgaaaata tcggtcgagg gcagggaaac tacgcgtaca ttgcgacctt
    52201 ctcccgctaa tttctctgcc gcttgcaggg tgatttccat ctctgaaccg gtggcaatca
    52261 gaataatatc tggcttaccg ccgctgtctt tcagcacata accgccacga gcaatctctt
    52321 taacctgatc cggcgtacgt tccacctggg ccagattctg ccttgagagg atcagtgctg
    52381 tcggcccgtt gtggcgctca accgccagct tccagcccac cgccgcttcc acctgatcgc
    52441 acggtcgcca ggtgctgaaa ttcggcgtta agcgcagact ggccagttgc tcaacggcct
    52501 ggtgcgtcgg accatcttcc cctaagccaa tagaatcgtg ggtataaacc ataatctgcc
    52561 gtgctttcat cagtgccgcc atccgcgcgg cattacgggc gtattcaaca aacatcagga
    52621 acgtcgcggt atacggcaca aagccgccgt ggtgcgcaat gccgttggcg atagcggtca
    52681 tgccaaattc acgcaccccg tagtgaatgt agttgcccgc cggatcttcc ttcagcgaca
    52741 cagagccttt ccagatggtg aggttgctgg gagccagatc cgctgaacct ccgagcagct
    52801 ccggtagcat cggtccgtaa gcgttaagcg tattttgcga agccttacgg gtggcgattt
    52861 tcgccggatt cgcctgcaac tcattgatat atttctgagt cgttttctcc cagtccttcg
    52921 gtaaaccacc gctcatccga cgggtaaact cttctgccag ttgcggatga gcctttttat
    52981 aggcggcaaa cttctcattc cagctctgct gcgctttttc gcctttttcg cgggcatccc
    53041 aggcgtgata aatctcttta gggatctcaa atggcggatg gtgccagccc agtttttgcc
    53101 gtgccagcgc cacttcttct tcccccagag gtgcgccgtg cgcctcttcc ttacctgctt
    53161 tattcggcga accaaagcca atcaccgtac ggcagataat cagcgacggc ttatctttca
    53221 cgctttgcgc ttcaaggatc gcttccttca ccgcctgcgg atcgtgaccg tcgatttcat
    53281 ggatcacatg ccagtgatag gcttcaaaac gttttgccgt atcgtcggta aaccagcctt
    53341 ctgtttcacc gtcgatggaa ataccgttgt gatcgtaaaa accaatcagc ttgcccagtc
    53401 ccagcgtgcc cgccagcgaa cagacttcgt gggaaatacc ttccatcagg cagccgtcgc
    53461 ccataaacac ataggtgaag tgatcgacaa tttcatgatc cggctgatta aactgcgccg
    53521 ccagtgtacg ctctgctatc gccagtccga cggcgttcgc caaaccttgt ccaagcggac
    53581 cggtggtggt ttcaacacct ggcgtatagc caatctccgg gtggcctggg gttttcgaat
    53641 gcaactgacg gaagttcttc agttcttcca gcggcaggtc gtaaccggtc agatgtagca
    53701 aactgtagag cagcatcgac gcgtgaccgt tggaaagaat aaagcggtcg cgatcatacc
    53761 aggttgggtc ggtaggatta tgtttaagaa aatcgttcca cagcacttcg gcaatatcag
    53821 ccatgcccat cggcgcgccg ggatgaccag aattggcttt ttgtaccgca tccatactga
    53881 gtgcgcgaat cgcattggca aggtcttttc gggacatata acactccgtg gctggtttat
    53941 agtttggcgg caagaagatc ttccagtttg cgttgatcaa cagcgaacag acgaatgcct
    54001 tcagacagtt tttctaccgc catcgcatcc tgattgtgct cccaacggaa ctccgcttcg
    54061 ctcatgggag ctgggcgtgg gaacgtctga gaaggtggga ttaatttacg taccactggc
    54121 gaaacttttt cctgcagctc cttcagcaaa ttcggtgcga tagtcagtcg atcgcagccg
    54181 gttaaggcga ggatttgttc ggtgcgacgg aagctcgcgc ccatcacaat ggtttcatag
    54241 tggtgttgct tatagtagtc gtagatattg cgcaccgatt taacgcccgg atcttcttcc
    54301 accacatacg ggtccatcgg cttgcgtgcc tgataccagt cataaatacg cccgacaaac
    54361 ggcgaaatca gaaaaatgcc cgcttctgca caggcccgcg cctgggcaaa agaaaacagc
    54421 agcgtcaggt tgcagttaat accttctttt tccagctctt cggccgcgcg aattccttcc
    54481 caggtcgaag ccagtttgat caaaatgcgt gatttctcaa cgccttgttg ctgatacaag
    54541 tccaccagat ggcgcgcctt ctcaatactc ttttctttat caaaagagag gcgtgcatca
    54601 acttctgttg acacgcgacc gggtacgatt ttgagaattt cagcaccgaa attgactgcc
    54661 agtttgtcac acgctgcgac cacctgttgt tcctgggtct tgccattttt tttaccccag
    54721 gcgatagcat cgtctattaa atgctcatat tgtgataatc cggcagcttt gagtaacagc
    54781 gaaggattgg tggtggcatc ctggggatga taatggcgaa tggactcaat atcgccgctg
    54841 tctgccacga cagtggtgaa ctgtttgatg ccgtctaact cgttcatagg aaatactcct
    54901 tgaaaagtaa agtgttagat gagtgcgtta attcacactt ctgagaaatt tcgctaaacg
    54961 catcaaaaaa gcatagcaga caggcatggt attgctggat taagcaggta acatcagtgt
    55021 tataggatta ttaccaaaac attatattaa tggtgagagt ttggacttgc tcaaagtctg
    55081 tagactccgg cagggtaata atgtgcgcca cgttgtgggc aggggctgtt gcccacacac
    55141 tttatttgtg aacgttacgt gaaaggaaca accaaatgga tgaccagtta aaacaaagtg
    55201 cacttgattt ccatgaattt ccagttccag ggaaaatcca ggtttctcca accaagcctc
    55261 tggcaacgca gcgcgatctg gcgctggcct actcaccagg cgttgccgca ccttgtcttg
    55321 aaatcgaaaa agacccgtta aaagcctaca aatataccgc gcgcggtaac ctggtggcgg
    55381 tgatctctaa cggtacggcg gtgctggggt taggcaacat tggcgcgctg gctggcaaac
    55441 cggtgatgga aggcaagggc gttctgttta agaaattcgc cgggattgat gtatttgaca
    55501 ttgaagttga tgaactcgac ccggacaaat ttattgaagt tgtcgctgcg ctcgaaccga
    55561 ccttcggcgg aatcaacctc gaagacatta aagcgccaga atgtttctat attgaacaga
    55621 aactgcgcga gcggatgaat attccggtat tccacgacga tcagcacggc acggcaatca
    55681 tcagcactgc cgccatcctc aacggcttgc gcgtggtgga gaaaaacatc tccgacgtgc
    55741 ggatggtggt ttccggcgcg ggtgccgcag caatcgcctg tatgaacctg ctggtagcgc
    55801 tgggtctgca aaaacataac atcgtggttt gcgattcaaa aggcgttatc tatcagggcc
    55861 gtgagccaaa catggcggaa accaaagccg catatgcggt ggtggatgac ggcaaacgta
    55921 ccctcgatga tgtgattgaa ggcgcggata ttttccttgg atgttccggc ccgaaagtgc
    55981 tgacccagga aatggtgaag aaaatggctc gtgcgccaat gatcctggcg ctggcgaacc
    56041 cggaaccgga aattctgccg ccgctggcga aagaagtgcg tccggatgcc atcatttgca
    56101 ccggtcgttc tgactatcca aaccaggtca acaacgtcct ttgcttcccg tttatcttcc
    56161 gtggcgcgct ggatgttggc gcaacggcta tcaatgaaga gatgaaactg gcggcggtac
    56221 gcgcgattgc ggaactggcc cacgccgaac agagcgaagt ggtggcttca gcgtatggcg
    56281 atcaggatct gagctttggt ccggaataca tcattccaaa accgtttgat ccgcgcttga
    56341 tcgttaagat cgctcctgcg gtcgctaaag ccgcgatgga gtcgggcgtg gcgacgcgtc
    56401 cgattgctga tttcgacgtc tacatcgaca agctgactga gttcgtttac aaaaccaacc
    56461 tgtttatgaa gccgattttc tcccaggctc gcaaagcgcc gaagcgcgtt gttctgccag
    56521 aaggggaaga ggcgcgcgtt ctgcatgcca ctcaggaact ggtaacgctg ggactggcga
    56581 aaccgatcct tatcggtcgt ccgaacgtga tcgaaatgcg cattcagaaa ctgggcttgc
    56641 agatcaaagc gggcgttgat tttgagatcg tcaataacga atccgatccg cgctttaaag
    56701 agtactggac cgaatacttc cagatcatga agcgtcgcgg tgtcactcag gaacaggcgc
    56761 agcgggcgct gatcagtaac ccgacagtga tcggcgcgat catggttcag cgtggggaag
    56821 ccgatgcaat gatctgcggt acggtgggcg attatcatga acattttagc gtggtgaaaa
    56881 atgtctttgg ttatcgcgat ggcgttcaca ccgcaggtgc aatgaacgcg ctgctgctgc
    56941 cgagtggtaa cacctttatt gccgatacct acgtcaatga cgaaccagac gcagaagagc
    57001 tggcggagat caccttgatg gcggcagaaa ctgtccgtcg ttttggtatt gagccgcgcg
    57061 ttgctctgtt gtcgcactcc aactttggtt cttctgactg cccgtcgtcg agcaaaatgc
    57121 gtcaggcgct ggaactggtc agggatcgtg cgccagatct gatgattgat ggtgaaatgc
    57181 acggcgatgc agcgctggtg gaagcgattc gcaacgaccg tatgccggac agccctttga
    57241 aaggttctgc caatattctg gtgatgccga acatggaagc tgcccgcatt agttacaact
    57301 tactgcgcgt ttccagctcg gaaggtgtga ctgtcggtcc ggtgctgatg ggcgtggcga
    57361 aaccggttca cgtgttaacg ccgatcgcat cggtgcgtcg tatcgtcaac atggtggcgc
    57421 tggccgtggt tgaagcgcaa acccaaccgc tgtaattctt ttttaactct cacgcttacc
    57481 ctgaatattc agggtaagcg gtttggctgc aatatattag taaagcttat tactgagttt
    57541 gcgaataata aaaaaagcag tctatataat atctcgatat tattttattt atattcatgc
    57601 gttgcatatg aaagtttatg caccacagcg aatatctctc cattcttagt gatctacctc
    57661 accttttaaa cgcgcttgcc gaattttgtt atttactctg acgaaaaatt gtcacgatac
    57721 acgaaagttt ttcacaggcg gcgactcatg gataaagaac gcatcattca ggaatttgtg
    57781 cccggcaaac aggtcacact ggcgcatctc atcgcgcatc ccggtgagga actggcgaag
    57841 aaaatcggcg ttccggatgc gggcgcaatt ggcattatga cgctaactcc cggcgaaacg
    57901 gcgatgatcg ccggtgattt agcgctgaaa gccgctgatg tgcatatcgg ttttctcgac
    57961 cgcttcagcg gtgcattggt ggatttatgg ctcggtggcg cagtagaaga agcgttatca
    58021 caaaccgtca gcggtttagg tcgcttatta aattatacgc tttgtgaaat gaccaaaagt
    58081 taatacgagg cattcatgaa acgaattgcg tttgtgggta ccgtcggggc aggaaaaaca
    58141 acgttattta atgcgttgca ggggaattat accctcgcca gaaaaacaca ggccgtggaa
    58201 tttaatgata agggcgatat tgatacgccg ggtgaatatt ttagccatcc ccgctggtat
    58261 cacgccttaa ttaccacgct acaggatgta gacatgctga tctatgtcca tggcgcaaat
    58321 gacccggaaa gtcggttgcc tgccgggttg atggatattg gcgtcagcaa acggcaaatc
    58381 gccgtcatca gtaaaacgga catgccagat gctgacgtcg ccgcaacgcg aaagttattg
    58441 cttgagacgg gtttcgaaga gccgattttc gagctaaaca gccacgaccc gcaaagcgtg
    58501 cagcaactgg tggattatct ggcatctctc accaaacagg aggaagcagg tgaaaaaact
    58561 catcacagcg aatgatattc gtgaagcaca cgcgcgcggc gaacaggcaa tgtccgtggt
    58621 tctgcgcgcc agcattatta ccccggaagc ccgtgaagtg gcggatctac tgggttttac
    58681 catcaccgaa tgtgatgaat cgatcccggt aacggcgtcc gtgcccgcca gcgtgccagc
    58741 cgacaaaacc gaaagccagc gcatccgcga aaccatcatt gcccagctgc cggaaggcca
    58801 gtttaccgaa agcctggtcg cacagctgat ggaaaaagtg atgaaggaaa aacagtcgct
    58861 ggagcagggc gcaatgcagc cgagcttcaa atcggtgacc ggcaaaggcg gcatcaaagt
    58921 gattgatggc agcagcgtca agtttggtcg tttcgacggc gcagagccgc attgcgtggg
    58981 cttgactgat cttgtgaccg gagacgacgg cagcagcatg gccgctggtt ttatgcagtg
    59041 ggagaacgca ttcttcccgt ggaccctgaa ctacgacgaa atcgacatgg tgctggaagg
    59101 ggagctgcat gttcgccacg aaggcgaaac catgatcgcc aaagcgggtg acgtgatgtt
    59161 tatcccgaaa ggctccagta tcgaatttgg tacgacatcc agcgtgaaat tcctgtatgt
    59221 cgcctggccg gctaactggc aatccctatg aaagatttca tcaccgaagc atggctaaga
    59281 gcgaaccata cgctcagcga aggcgcagag atccatctcc ctgcggacag tcgcctgacg
    59341 ccctctgccc gggagttact ggaaagccgc catctgcgca tcaagtttat tgacgagcag
    59401 ggccgcctgt ttgttgacga tgaacagcag cagccgcagc ccgttcatgg gctgaccagt
    59461 agcgatgaac atccgcaggc gtgctgcgaa ctgtgccgcc agccggtggc aaaaaagccg
    59521 gatacgctga ctcacctgtc ggcggaaaaa atggtcgcca aaagcgatcc gcgtctgggt
    59581 tttcgcgcgg ttctcgacag caccattgcg ctggcagtgt ggctgcaaat tgaactggcg
    59641 gaaccgtggc agccgtggct ggcggatatc cgttcgcgtc ttggcaacat tatgcgcgcc
    59701 gatgcgctgg gtgaaccgct gggctgccag gcgattgtcg ggctttctga cgaagatctg
    59761 catcgacttt ctcaccagcc gctgcgctat ctcgaccacg atcatctggt gccggaagcc
    59821 agccacgggc gcgatgcggc gttgctcaat ctgctacgta ccaaagtgcg ggaaaccgaa
    59881 accgtagcgg cgcaggtgtt tatcacccgc agctttgaag tattacgccc ggacattttg
    59941 caggcgctga accgcctttc cagcacggtc tacgtgatga tgatcctgag cgtaacgaag
    60001 cagccgctaa cggtgaagca gatccaacaa cgactggggg aaacgcaatg attattgaac
    60061 gttgtcgtga actggcgttg cgagcgcccg ccagagtggt ttttccggat gcgttagacc
    60121 aacgtgtgct gaaagccgcg caatatttac atcaacaagg tctggcaacg cccattctgg
    60181 tcgccaatcc gtttgaactt cgtcagtttg cgctcagtca cggcgtggcg atggacgggc
    60241 tacaggtgat tgatccgcat ggcaacctcg caatgcggga agaatttgct catcgctggc
    60301 tggcccgcgc gggcgaaaaa acgccgccgg atgcgctgga aaaacttacc gaccctctga
    60361 tgttcgccgc agcaatggtc agcgccggta aagcggatgt ctgtatcgcg ggcaacctct
    60421 cttccacggc aaatgtgctg cgtgccggat tacgcattat cggcttgcag ccaggctgta
    60481 aaacgctctc ttccattttc ctgatgttgc cacagtacag cggcccgccg ttgggctttg
    60541 ccgattgcag cgtggtgcca cagccgacgg cggcgcagct ggcggatatc gcgcttgcca
    60601 gtgccgaaac ctggcgcgcc atcaccggag aagagccgcg cgtggcgatg ctgtcgtttt
    60661 ccagcaatgg tagcgcccgt cacccctgtg ttgctaacgt ccagcaggcg acagaaatcg
    60721 tccgtgagcg cgcaccaaag ctggtggtgg atggcgagtt acagtttgac gccgccttcg
    60781 tgccggaagt ggcggcgcaa aaagcgcctg ccagcccgct acagggcaag gccaatgtga
    60841 tggtttttcc gtcgctggaa gccggaaata ttggttacaa aatcgcacaa cgactcggcg
    60901 gatatcgtgc cgtcgggcca ctgatacaag gacttgccgc gccgatgcac gatctctctc
    60961 gtggttgtag cgtgcaggaa attatcgagc tggcgctggt ggcagctgtg ccgcgtcaga
    61021 cagaagtgaa ccgcgaaagc agtttacaaa cactggttga atgaacggtc ccgttctgga
    61081 ccctgataaa gaggaaagca caatggaagc attaggaatg atcgaaaccc ggggcctggt
    61141 tgcactgatt gaggcttctg acgcgatggt gaaagctgcg cgcgtcaagc tggttggtgt
    61201 taagcagatt ggcggcggcc tgtgtactgc catggttcgt ggtgatgttg cagcctgtaa
    61261 agccgcgacc gatgcaggtg ccgcagcggc acagcgtatc ggcgagctgg tatctgtaca
    61321 cgttatcccg cgtccgcacg gcgacctgga agaagtcttc ccgatcggcc tgaaaggcga
    61381 tagcagcaac ctgtaatcac cctgtgtgac gtcgattcgg gcgtcacgct ctctcctttt
    61441 tcattttacc ttctgcggag ggtaggggga gcttttacct gaaatacgcc acggaggcgg
    61501 gtatgaaact ggcagtcgtc actggacaaa ttgtttgtac cgtacgccat cacggactgg
    61561 cgcatgacaa attgctgatg gtggaaatga ttgatccaca aggtaatcct gacgggcaat
    61621 gcgccgtcgc catcgacaat attggcgcgg gaaccgggga gtgggtgtta ctggtgagtg
    61681 gcagttccgc ccgccaggcg cataaaagcg aaacgtcacc ggtcgatctg tgcgtgattg
    61741 gcattgtcga tgaggtggtg tctggcggtc aggtaatttt ccacaaataa ggcagaacat
    61801 catgaatcaa caggatattg aacaggtggt gaaagcggta ctgctgaaaa tgcaaagcag
    61861 tgacacgccg tccgccgccg ttcatgagat gggcgttttc gcgtccctgg atgacgccgt
    61921 tgcggcagcc aaagtcgccc agcaagggtt aaaaagcgtg gcaatgcgcc agttagccat
    61981 tgctgccatt cgtgaggcag gcgaaaaaca cgccagagat ttagcggaac ttgccgtcag
    62041 tgaaaccggc atgggccgcg ttgaagataa atttgccaaa aacgtcgctc aggcgcgcgg
    62101 cacgccaggc gttgagtgtc tctctccgca ggtgctgacc ggcgacaacg gcctgacttt
    62161 aattgaaaac gcgccctggg gcgtggtggc ttcggtaact ccgtccacta acccggcggc
    62221 aaccgtaatt aataacgcca tcagcctgat tgccgcgggc aacagcgtca tttttgcccc
    62281 gcatccggcg gcgaaaaaag tctcccagcg ggcgattacg ctgctcaacc aggcgattgt
    62341 tgccgcaggt gggccggaaa acttactggt tactgtggca aatccggata tcgaaaccgc
    62401 gcaacgcttg ttcaagtttc cgggtatcgg cctgctggtg gtaaccggcg gcgaagcggt
    62461 agtagaagcg gcgcgtaaac acaccaataa acgtctgatt gccgcaggcg ctggcaaccc
    62521 gccggtagtg gtggatgaaa ccgctgacct cgcccgtgct gctcagtcca tcgtcaaagg
    62581 cgcgtctttc gataacaaca tcatttgtgc cgacgaaaag gtactgattg ttgttgatag
    62641 cgtagccgat gaactgatgc gtctgatgga aggccagcac gcggtgaaac tgaccgcaga
    62701 acaggcgcag cagctgcaac cggtgttgct gaaaaatatc gacgagcgcg gaaaaggcac
    62761 cgtcagccgt gactgggttg gtcgcgatgc ggcgaaaatc gcggcggcaa tcggcctgaa
    62821 cgtgccgcaa gaaacgcgtt tgctgtttgt ggaaaccact gcagaacatc cgtttgccgt
    62881 gaccgaactg atgatgccgg tgctgcccgt cgtgcgcgtc gccaacgtgg cggatgccat
    62941 tgcgctggcg gtgaaactgg aaggcggttg ccaccacacg gcggcaatgc actcgcgcaa
    63001 catcgaaaac atgaaccaga tggcgaatgc tattgatacc agcattttcg ttaagaacgg
    63061 accgtgcatt gccgggctgg ggctgggcgg ggaaggctgg accaccatga ccatcaccac
    63121 gccaaccggt gaaggggtaa ccagcgcgcg tacgtttgtc cgtctgcgtc gctgtgtatt
    63181 agtcgatgcg tttcgcattg tttaaggagt aacagatggc gcacgacgaa caatggctca
    63241 ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac gcccgccgcg acggaatctc
    63301 cgctgtggct gggggtggat ctgggcacct gcgatgtggt gtcgatggtt gtcgaccgcg
    63361 acggtcagcc ggtggcggta tgcctcgact gggccgatgt ggtccgcgac ggcatcgtct
    63421 gggatttctt cggcgctgtc accattgttc gtcgccatct cgacacgctc gagcagcaat
    63481 tcggtcgccg ttttagccat gcggcgactt catttcctcc aggtacggac ccgcgtattt
    63541 ccatcaacgt gctggagtct gccgggctgg aagtgagcca tgtgctggat gagccaaccg
    63601 cagtcgcgga tctactgcaa ctggataacg caggcgtagt ggatatcggc ggtggcacta
    63661 ccggcattgc catcgtgaaa aagggcaaag tgacgtactc ggcggatgaa gccaccggcg
    63721 gacatcacat ctctctgact ctcgccggaa atcgccgtat ttcgctggaa gaggcggagc
    63781 agtacaagcg cggtcacggt gacgagattt ggcctgcggt gaaaccggtg tacgaaaaaa
    63841 tggcggacat tgtcgcccgg catattgaag ggcagggcat tactgattta tggctggcgg
    63901 gcggctcctg tatgcaaccg ggcgtggcag aactgtttcg caaacaattc ccggcgttac
    63961 aggtgcattt accgcagcac agtttgttta tgaccccgct ggcgatcgcc agtagtgggc
    64021 gagagaaagc ggaggggcta tatgcaaaat gaattgcaga ccgcgctctt tcaggcgttc
    64081 gataccctga atctgcaacg ggtaaaaaca tttagcgttc caccggtgac gctttgcggt
    64141 ctgggcgcgg tgagcagttg cgggcagcaa gcgcaaacgc gtgggctgaa acatctgttc
    64201 gtgatggcag acagcttttt gcatcaggca gggatgaccg ccgggctgac gcgcagcctg
    64261 gccgttaaag gcatcgccat gacgctctgg ctatgtccgg tgggcgaacc gtgcattacc
    64321 gacgtgtgtg cagccgtggc gcagttgcgt gagtcaggtt gtgacggggt gatcgcattt
    64381 ggcggcggct cggtgctgga tgcggcgaaa gccgtggcgt tgctggtgac gaacccggat
    64441 agcacgctgg cagagatgtc agaaaccagc gttctgcaac cgcgcttgcc gctgattgcc
    64501 attccaacta ccgccggaac cggctctgaa accaccaatg taacggtgat tatcgacgcg
    64561 gtgagcgggc gcaagcaggt gttagcccat gcctcgctga tgccggatgt ggcgatcctc
    64621 gacgccgcat tgaccgaagg tgtgccgtcg catgtcacgg cgatgaccgg cattgatgcg
    64681 ttaacccatg ccattgaagc atacagcgcc ctgaacgcta caccgtttac cgacagtctg
    64741 gcgattggtg ccattgcgat gattggcaaa tcgctgccga aagcggtggg ctacggtcac
    64801 gaccttgccg cgcgcgagag catgttgctg gcttcatgta tggcgggaat ggcgttttcc
    64861 agtgcgggtc ttgggttgtg ccacgcgatg gcgcatcagc cgggcgcggc gctgcatatt
    64921 ccacacggtc tggcgaacgc catgttgctg ccaacggtga tggaatttaa ccggatggtt
    64981 tgtcgcgaac ggtttagtca gattggtcgg gcactgcgaa ctaaaaaatc cgacgatcgt
    65041 gacgctatta acgcggtaag tgagctgatt gcggaagttg ggattggtaa acgactgggc
    65101 gatgttggcg cgacatctgc gcattacggt gcatgggcgc aggccgcgca ggaagatatt
    65161 tgtctgcgca gcaacccgcg taccgccagc ctggaacaga ttgtcggact gtacgcggcg
    65221 gcgcaataaa tgccggatgc gacgcttgcc gcgtcttatc cggcctacgg gattgcacat
    65281 gtagggcgga taaggcgttt acgccgcatc cgccaataaa taacaactaa cagggagtaa
    65341 aggcgatggg aattaacgaa atcatcatgt acatcatgat gttctttatg ctgatagctg
    65401 ccgtagacag gatcctgtcg cagttcggcg gttctgctcg tttcctcggt aagttcggta
    65461 aaagtatcga aggatcaggc ggtcagttcg aagaaggctt tatggcaatg ggcgctctgg
    65521 gtctggcgat ggtcggtatg accgcgctgg caccggtact ggctcacgta ctcgggccgg
    65581 taattatccc ggtttacgaa atgctcggcg caaacccgtc aatgttcgcc ggaacactgc
    65641 tggcgtgcga tatgggcggc ttcttcctcg ccaaagagct ggcgggcggc gacgtagcag
    65701 cgtggctata ctctgggtta attctcgggt cgatgatggg gccaacgatt gtgttttcca
    65761 ttccggtggc gctcggcatt atcgaacctt ctgatcgtcg ttatctggcg ctcggcgtgc
    65821 tggcgggcat tgtgaccatt ccgattggct gtattgctgg tggtctggtt gctatgtact
    65881 ccggtgtgca gatcaacggt cagccggtgg aattcacttt cgccctgatc ctgatgaaca
    65941 tgatcccggt gatcattgtc gcgattctgg tggcgctggg gctgaaattc atcccggaaa
    66001 aaatgatcaa cggcttccag atctttgcca aattcctcgt tgcattgatc accctcggtc
    66061 ttgccgctgc ggtagtgaaa ttcctccttg gctgggaact gatccccgga ctggatccta
    66121 tctttatggc ccctggcgat aaacccggtg aagtgatgcg cgccattgaa gttatcggtt
    66181 ctatctcctg cgtgctgtta ggggcgtatc caatggtgct gctgctgact cgctggtttg
    66241 aaaaaccgct gatgagcgtc ggtaaagtac tgaatatgaa caacatcgcg gcagccggca
    66301 tggtggcaac gcttgccaac aacatcccga tgttcggcat gatgaagcag atggataccc
    66361 gcggcaaagt catcaactgc gccttcgccg tttccgctgc tttcgccctg ggcgaccact
    66421 taggcttcgc cgctgccaac atgaacgcca tgatcttccc gatgattgtc ggcaagttga
    66481 tcggcggcgt aacggcgatt ggcgtggcga tgatgctggt gccaaaagaa gacgcgaccg
    66541 cgactaaaac cgaagcggag gcacaatcgt gaacactcgc cagctattga gcgtcggtat
    66601 cgatatcggc accaccacca cccaggtgat tttctcccac ctggaactgg ttaaccgtgc
    66661 ggcggtgtcg caggtgccgc gctacgaatt cattaaacgc gaaattagct ggcaaagccc
    66721 ggtattcttt acccctgtcg ataaacaggg tggtttaaaa gaagcggaac tgaaatcgct
    66781 gattttagag caatatcagg ctgcgggcat tgcgccggaa agcgttgatt ctggtgccat
    66841 catcatcacc ggtgaaagcg cgaaaacccg caatgctcgc ccggcggtga tggcgctctc
    66901 tcagtcgctg ggcgattttg tcgttgccag cgccgggccg cacctcgaat ccgtgatcgc
    66961 cggtcacgga gccggggcac aaaccctttc tgaacaacgg ctgtgtcggg tactgaatat
    67021 cgacatcggc ggtggcaccg cgaactacgc cctgttcgat gccggaaaaa tcagcggcac
    67081 tgcctgtctc aacgtcggtg gtcgcctgct ggaaaccgac agccaggggc gcgtggttta
    67141 cgctcataaa ccggggcaga tgattgtgga tgagtgcttc ggtgcaggca ctgacgcccg
    67201 ttcgctgacc ggcgcgcagc tggtgcaggt tacccggcgg atggcggcgc tgattgtcga
    67261 agtgattgac ggaacgcttt cgccgctcgc gcaggcattg atgcaaaccg gtttgctgcc
    67321 cgcaggtgtt acgcccgaaa tcattacgct ttctggaggc gtgggcgaat gttatcgcca
    67381 ccagcccgcc gacccgttct gttttgccga tattggccca cttctggcaa cggcgctgca
    67441 tgaccatccg cgcctgcgtg agatgaatgt gcagtttccg gcgcaaaccg tacgcgccac
    67501 ggtgattggc gcgggtgcac ataccctttc gctctctggc agcacaatct ggctggaggg
    67561 cgtacaactg ccgctgcgca atttgccggt ggcgatcccg attgatgaaa cggatctggt
    67621 gagtgcctgg caacaggcgc tgcttcagct ggatcttgat cccaaaactg acgcgtacgt
    67681 gctggcgctt cccgcctcgc tgcctgtgcg ttacgccgcg gtactgacgg tcatcaacgc
    67741 gctggtcgat ttcgtcgcgc gttttccgaa tccgcatccc ctgctggtgg tggccgggca
    67801 ggactttggt aaagctctgg gcatgttgtt gcgcccacag ctacaacaac tcccgttggc
    67861 agtcattgac gaagtgattg tccgcgcggg ggactatatc gacattggta cgcctctttt
    67921 tggcggatcg gttgtgccgg tgacggtgaa atcactcgca tttccttcct gagggaacga
    67981 cttatgaaac taaagaccac attgttcagc aatgtatatc agtttaagga tgtaaaagag
    68041 gtgctggcta aagccaacga actgcgttcg ggggatgtgc tggcaggcgt tgcagcggca
    68101 agttcacagg agcgcgtggc ggcaaagcag gtgttgtcgg aaatgaccgt agcggacatc
    68161 cgcaataatc cggtgattgc ctatgaagat gactgcgtga cgcggctgat tcaggacgat
    68221 gttaacgaaa cggcctacaa ccagattaaa aactggagca tcagcgaact gcgtgagtat
    68281 gtgctgagcg atgaaaccag cgtggacgac attgccttta cccgcaaagg gctgacctcg
    68341 gaagtggtcg cggcggtagc gaagatttgc tccaacgcgg acctgatcta cggcgcgaag
    68401 aaaatgccgg taatcaaaaa ggccaatacc accattggta ttccgggcac cttcagcgcc
    68461 cgtttgcagc caaatgacac ccgtgacgac gtgcaaagta tcgccgcgca aatctacgaa
    68521 gggctttcct tcggggtggg cgatgcggtg atcggcgtta acccggtgac tgacgacgtg
    68581 gaaaacttaa gccgcgtgtt ggacaccatt tacggcgtga tcgacaaatt caacatccca
    68641 actcagggct gcgtactggc gcacgtcacc acccagatcg aagcgattcg tcgcggcgcg
    68701 cctggcggac tgattttcca gagtatctgt ggcagcgaaa aagggctgaa agagtttggc
    68761 gtggaactgg cgatgctcga cgaagcgcgc gcagtgggtg cagagttcaa ccgtatcgcc
    68821 ggggaaaact gcctctactt cgaaaccgga caaggctctg cgctatccgc tggcgctaac
    68881 ttcggcgctg accaggtgac gatggaagca cgtaactatg ggctggcgcg tcattacgat
    68941 ccgtttatcg tcaacaccgt ggtcggcttt attgggccgg agtatctcta caacgaccgc
    69001 cagattatcc gtgctggctt agaagatcac tttatgggca agctgagcgg catctctatg
    69061 ggctgtgact gctgttacac caaccacgct gacgctgacc agaacctcaa cgaaaacctg
    69121 atgatcctgc tcgccaccgc aggctgcaac tacatcatgg ggatgccgct gggtgatgac
    69181 atcatgctca actaccagac caccgcattc cacgataccg ccactgtgcg tcagttactc
    69241 aacctgcgtc cgtcaccgga gtttgaacgc tggctggaaa gcatgggcat tatggcaaac
    69301 ggtcgcctga ccaaacgggc gggcgatccg tcactgttct tctgatgacg cggagataac
    69361 acatcatgga tcaaaaacag attgaagaaa ttgtacgcag cgtgatggcg tcaatgggac
    69421 aaacggcccc cgcgccgtca gaagcaaagt gcgccaccac caactgtgcg gcaccggtga
    69481 cctcggaaag ctgtgcgctg gatttaggtt ccgctgaagc aaaagcgtgg attggtgttg
    69541 aaaacccgca tcgcgcagac gtattaacag aactgcgccg cagcaccgtg gcccgcgttt
    69601 gtaccggtcg tgccggtccg cgtccacgta cccaggcgct gctgcgtttc ctggccgatc
    69661 actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg aaagcgcagg
    69721 gcttgctgga agtacgctct gaaattagcg acaaaaatct ctacctgact cgcccggata
    69781 tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt gttgctaatc
    69841 cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc gtgaactacg
    69901 aagagatcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa gtcggcacgc
    69961 cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatcggtgag atcctcggcg
    70021 cgaaagtggt gatcctgctg gtgggggaac gtccggggtt agggcagtca gaaagcctct
    70081 cctgctacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat cgcacctgta
    70141 tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc attgtggatt
    70201 tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga taaggaggca
    70261 tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg attgcctccg
    70321 ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc ctcgggctga
    70381 tttctgctga ttccgatgac gtcacctaca ttgccgctga tgaagcgacc aagcaggcga
    70441 tggtggaagt ggtatatggt cgctcgctgt atgcgggcgc tgcacacggc ccgtcaccga
    70501 ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg cgtgccggtc
    70561 tggatgcgat ggttgcacat attgaaaatg gtgcagcatt ccagtgggcc aatgacgcag
    70621 aaaacaccgc cttcctggcg catgtggttt cgcgtaccgg ttcttatctc tcatcaaccg
    70681 ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg gaagcgacct
    70741 atggcattga tgcagcgttg aaatctgccg acgtacagct ggtgacctat gtcccgccac
    70801 cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg tgtaaagcag
    70861 cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc cagcgtgcgt
    70921 aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc atggtcgccg
    70981 cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc agtcacgaag
    71041 tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg tgtcgtgcgg
    71101 cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc cgcaaggaga
    71161 tcggtcggcc agacgatgac acccagtggc tggttactgg ctttaaccgc cagccgcagc
    71221 aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga atctgctgac gagttgttgg
    71281 cgctgttaac atcagtacgt cagggaatga cggcaggaga agtggctgcc cactttggct
    71341 ggccgctgga aaaagccaga aatgcgctcg aacagctctt ttctgccggg acgttacgta
    71401 aacgcagtag tcgttatcgt ctcaagcccc attaacctgt cggaggtgcc gggtgtccta
    71461 caacacccgg cattaacatc atgaaaaaga cccgtacagc caatttgcac catctttatc
    71521 atgaaccctt acccgaaaac ctgaagctca cgccgaaggt cgaagtggat aatgttcatc
    71581 aacgacagac aacggatgtc tatgaacatg ctttaacgat taccgcctgg cagcagattt
    71641 acgatcagct gcatccgggc aagtttcatg gtgaatttac ggaaattcta ctcgatgata
    71701 ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg gtctggccga
    71761 actcgttctg gtttggcatt ccggcgacgc gcggtgagca gggatttatc ggttcgcaat
    71821 gtctgggaag cgcggaaatc gccacccgcc ctggtggcac tgaatttgaa ctgagcacgc
    71881 cggatgatta cacgatcctg ggcgtggtgc tttctgaaga tgtcatcacc cgacaggcta
    71941 actttttgca taacccggat cgggtattac atatgctgcg tagccagtcg gcgctggaag
    72001 tgaaagagca gcataaagcc gcgctgtggg gctttgtcca acaggcgctg gcgacgtttt
    72061 gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg gataatttgc
    72121 taatggcgat gggggcgatg ctggaagacg cgcaaccaat ggtgacggcg gaaagcatca
    72181 gtcatcagag ttaccgtaga ttactttccc gcgcccgtga atatgtgctg gaaaatatgt
    72241 ctgagccggt gacggtgctg gacttgtgta atcaactgca tgtcagtcgc cgcacgctac
    72301 aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa cgcattcgcc
    72361 tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg gtaaaagacg
    72421 ccgccatgca gtggggattc tggcatctgg ggcaatttgc cacggattac cagcagctgt
    72481 ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg ggaatggggg tgagggagtt
    72541 acacccaatc cctgacctta ataaactcac ttaacgccgc ttctgggctg ccatcttttg
    72601 gctgataatc atactcccag cgtaccagtg gcggcattga catcaggatc gactcggtgc
    72661 gcccgccagt ttgcaggcca aacagagtgc cgcgatccca gaccagattg aactcgacat
    72721 aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc atcgctttac
    72781 gtcgctctac aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc tgcataaagg
    72841 caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg ccaataccgc
    72901 gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca ccactttttg taacggggat
    72961 aaacgtcttc accaaatggc aggcacaggt cacgggcggt gcggtgccag tgaatggcgt
    73021 cttcttcaaa accatagaaa ggggttaaat cgaagccgcc gccaaaccac cagacgggct
    73081 ctgcacccgg tttttcggca ataaaaaaac gcacattcgc gtggctggtg ggaacatacg
    73141 ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc ccggcaagtt
    73201 ccgggcgatg agcggtggcg gaagctggca tcgcctcgcc gtggacgtga gaaaagttga
    73261 cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg actacgcccg ccgccgccag
    73321 cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc gacggcggtc agctgctgac
    73381 aaatcgtatc ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg ggtttcataa
    73441 cttatcgctt tttcgaatgt gctttctggt tgtcgaacca gtggaaataa ctgatcacgc
    73501 cttcagcaat cgctgtggcg attttctgac gaaacgccgc cgtgcctaac agccgctctt
    73561 cttccgggtt ggtgataaac gaggtttcca ccagcaccga aggaaccgac ggcgatttca
    73621 acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc ggcttaatct
    73681 ttttcagaat atgcgagccg agcgtcaggc tgtttttaat agtatcggtt tgcaccagat
    73741 caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg gcaacttcat
    73801 cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc gctacttgcc ccacggttag
    73861 agagggcaaa taccgaagca ccggcagctt tcgggttggt aaagccatcg gcatgaattg
    73921 acataaacag atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa agtgggataa
    73981 acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg attacgcaaa atggaacgga
    74041 cgtttttagc aatcgccagc accacatgtt tttctttcga accgttgcgt ccgatcgctc
    74101 cggtatcaat tccgccgtgg cctggatcga gaacaacgac acgtttgccg ccagattttt
    74161 tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg gcgatggctt
    74221 gcgacattcc tgacaacgtc agggcagcca aaccggcttt cagcacctgg cggcgcgaag
    74281 tgagtgtttt tagtggttta aaagtgctca tacggcctga gttgtaataa taaagttcca
    74341 gatgttatat cgtatcgcgt aatccgttac gacggtttga tgttgagaat tgttttactt
    74401 ttcatttcaa tagttgacaa ctccccatta tgccactttt tcaaccgatt ttcgtcagat
    74461 attggctaat tcgaccgttc acgccataat cagcctttta acccgcaaaa aggtaacgaa
    74521 taccatggag atacgcgtat ttcgccagga agatttcgaa gaggtcatca ccctttggga
    74581 gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc gtaagatgaa
    74641 ccatgacgtc agtttgtttt tggtcgctga ggtaaacggt gaagtggtcg gaacggtgat
    74701 gggcggttat gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc cagagtttcg
    74761 tgggcgtggg attgccaatg cgttgcttaa tcggctggag aaaaagctga ttgctcgtgg
    74821 ctgcccgaaa attcagatca atgtgccgga agataacgac atggtgctcg gaatgtatga
    74881 acgcctggga tatgaacacg ccgacgtgct gagtctgggt aagcgtttga ttgaagatga
    74941 agagtactga gttccatcct gtccattatg atgcgcacgg acgcctgcgt ttacccttgc
    75001 tcttctggct tgtgctattg cttcaggcgc gaacctgggt gctgtttgtc attgccggtg
    75061 cgtcgcgtga gcagggtact gcgctgctaa atctgtttta tcccgatcac gataatttct
    75121 ggctggggct gattcctggc attccggcgg tgctggcgtt tttgttgagc ggtcggcggg
    75181 ctacatttcc tcgcacttgg catgtgctct attttctgtt gctgttggcg caggtggttt
    75241 tactttgctg gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt atcggtctgg
    75301 cgctggtagt ggcggatatt gtggcgttaa tctggctgct gaccaatcga cgtttacgcg
    75361 cttgttttaa tgaagtaaaa gaataacggc actttttggt gaatttgcac tccaagcaac
    75421 gttattgaat aaccaaagga agtgacatga aatcgctgcg tttaatgtta tgcgctatgc
    75481 cattgatgct gaccggctgt tccacgatgt cgtcagttaa ctggtctgcc gctaacccgt
    75541 ggaactggtt tgggtcgtcc accaaagtga gcgagcaggg cgttggtgaa ttaacggcgt
    75601 ccacaccact gcaagaacaa gccattgccg atgcgcttga tggcgattat cgcctgcgca
    75661 gcggaatgaa aaccgcgaac ggcaacgtgg tgcgcttttt tgaagtgatg aagggcgaca
    75721 acgtggcgat ggtgattaac ggcgatcaag gcacgatcag tcgcattgac gtgctggata
    75781 gcgatattcc tgctgacacc ggtgttaaaa tcggtacacc gtttagcgac ctttacagca
    75841 aagcatttgg caattgccaa aaagctgatg gtgatgataa tcgtgccgtc gaatgtaaag
    75901 ccgaaggcag tcaacatatt agctaccagt tcagcgggga atggagtggt cctgaagggt
    75961 taatgccttc ggacgatacc ctgaaaaact ggaaagtcag taaaattatc tggcggcgtt
    76021 aatttgcgtc tgaacaaacc gccgccgcga aaaaacacgt aaaatagcgc tcaacaatgc
    76081 cacggattgc gtggcattat tattttcagg aggaacaatg tctcaggttc agagtggcat
    76141 tttgccagaa cattgccgcg cggcgatttg gatcgaagcc aacgtgaaag gggaagttga
    76201 cgccctgcgt gcggccagta aaacatttgc cgacaaactg gcaacttttg aagcgaaatt
    76261 cccggacgcg catcttggtg cggtggttgc ctttggtaac aacacctggc gcgctctgag
    76321 cggcggcgtt ggggcagaag agctgaaaga ttttccgggc tacggtaaag gccttgcgcc
    76381 gaccacccag ttcgatgtgt tgatccacat tctttctctg cgtcacgacg taaacttctc
    76441 tgtcgcccag gcggcgatgg aagcctttgg tgactgcatt gaagtgaaag aagagatcca
    76501 cggcttccgt tgggttgaag agcgtgacct gagcggcttt gttgacggta cggaaaaccc
    76561 ggcgggtgaa gagacgcgtc gcgaagtggc ggttatcaaa gacggcgtgg atgcgggcgg
    76621 cagctatgtg tttgtccagc gttgggaaca caacctgaag cagctcaacc ggatgagcgt
    76681 tcacgatcag gagatgatga tcgggcgcac caaagaggcc aacgaagaga ttgacggcga
    76741 cgaacgtccg gaaacctctc acctgacccg cgttgatctg aaagaagatg gcaaaggact
    76801 gaagattgtt cgccagagcc tgccgtacgg caccgccagt ggcactcacg gtctgtactt
    76861 ctgcgcctac tgcgcgcgtc tgcataacat tgagcagcaa ctgctgagca tgtttggcga
    76921 taccgatggc aagcgtgatg cgatgttacg tttcaccaaa ccggtcactg gcggctatta
    76981 cttcgcgccg tcgctggaca agttgatggc gctgtaagcg tttctgattc gatgatgaaa
    77041 ggccaggcag taacattact gactggcctt tttattactg ctttaacgcc gcatatacct
    77101 gatcaaccac ccaaccataa gttgcaattg gcaactgacc gctttcgaac atattgggat
    77161 tcttttgcgg atcggcaacc ggtgaatgtg gcttgttact taacatcaca atcgccatat
    77221 gattcaccgg atcgataacg gtcaccgttc cggtccagcc agtgtgaccg taggtttgcg
    77281 ggcttgccag cgtgccaaac gtcggcgtca tggtggcatt accatttacg cgccagccga
    77341 ggccaaaagt ggcatcttcc ttagagctgg tggtgaacat cttcaccgtt tccgcactga
    77401 acagctgcac atcgccatag cccccgccgt tcagcatcgt ttgcattaac accgcaatat
    77461 cgccggtatt ggaaaacaaa cctgcgtgcc cggaaacgcc gcccatcgaa taaaaggctt
    77521 tttcatcgtg cacctgaccc cagagagtgg aggtgcggat attcgggaaa tggatcacgc
    77581 catcgcgggt attgccgttc aattccgtgg cggcaatttg ttgcggttta aagcctttca
    77641 gcagcgggtt aaataccgta tgtgtcaggc cgagcgggcg ataaatcgac tcttcaacat
    77701 agcggtcaag cggctgaccg gtgaccgact cgacgataaa tccaagcagc atataatcga
    77761 catcactgta aatatgcttg ctgccgggct gatactctag cggcgtgcgc ttgatcatct
    77821 ccagcgtctg gcctttatcc tgggaatata acgcgcccgc gaccgcttta ttcgggtatt
    77881 gcggatctgc cgggaaaccg ccgctgtgat gcagcaggtc agaaatccgc agggtgtttt
    77941 tgcctttaat ggtgtcattt ggactatcgg caaaccccgg aatatatttc gcaatccgat
    78001 cgtcaggatg cagtttgcct tcgctcatca gcttttgcag ggcgaagttc gtggcgtaca
    78061 ttttggtgtt tgaggccagg tcatacagcg tcccggtggt ggctttgaca ggctgttcca
    78121 tcaacacgct gccatcgtac tttttcgccg ctccccaggc tttgcgatac acaatctgat
    78181 tatctttaat gatcagcagg tttacgccgg gataaccggc gtccacttgc tggctaatcc
    78241 agcgatccat ctggttaagc cgttcgacgt tgaaccctgc tttctctggc gaactttctg
    78301 tcagaacagg gtattttgcg gcgctgacgc tacaactaac agccagcaga gaaagataga
    78361 gcattgtccg tttcatgata tcagtccaga ttgacgttac ggcagccaaa gagcgtggtg
    78421 aaaataaacc cgcaaaccca tgccaccaga atcccgccag cataaaccgc cattgccgga
    78481 aggatgcctt gtgcggaagt catcagcggc agcgccacca gaccagacgg cccaaaggcg
    78541 ctgtttaagc ccatcggcag accccaccag gctatcaggc cgataaacaa accgcccgct
    78601 gcgccgccta aacaggcggt aataaacggt ttcatgcggg gcagggtgac gccgtaaatc
    78661 aacggttcac caacgcccag cagaccggga ataatcgccc cgcgtacctg actgcgtaat
    78721 gcactgtgcg gttgcgcccg ccagtagagt gccagcgccg cgcccacctg gcccgcgcct
    78781 gccattgaaa ggatgggaaa taagctgttg aatccctggc tgtccattaa cgcgaggtaa
    78841 acaggaataa agccctgatg cacgccaaat accacggcga tcaggaacag cccggctaaa
    78901 accgcacaac cgaacggatt actgttcagg tgcataaaca gccatgacat accttcgaat
    78961 aaccagccac ccagcggcat aatgatcagg tacgcaagcg tagcggtgat cagcagggtg
    79021 attaacgagg tcagcagcat gtcgagatcg tccggcataa agcggcgcac catgccttca
    79081 atgcgggcgc aggcccacgc ggcaatcagc acgccgataa tattgccgcg cggatcgatg
    79141 ggcagaccaa agaaatcgtg aaaaccggcg tagtaaccgg tggttgcagc ggggttataa
    79201 ccgagcaaaa agagtgcggc aataattgcg ccattgacgc ccgtgccgcc aaatgcctgg
    79261 gcggcgttat agcccaccag aatcaccagg aaagtgaaca aacctttgct gaacaccttc
    79321 ataaaattca gcgcgtcggg tagcgtgccc tgagcatctg ccggaacgtg catcaccgtg
    79381 gcaattaacg tcgctatccc cagcagcaga ccggcggcaa taaaaccggg gatcagcggc
    79441 gtgaagatgg tggcgaattt ggcgagaaat tgttgcacgc cggaggtttg tttggctttt
    79501 aactgacgtt tgttctgggc ggcgatttct gcggcatcct gtaccggtgc ctcgcccagc
    79561 agctcgctca tcgcttttgc ggcacgatgc gctttaccag gcccaaaaac gacctgcacc
    79621 tggtcgctgg tcaaaatgac gcccttcacg ccttccagcg ttttgatatt gggatcaacc
    79681 agtgaactgt catgtacacc cagacgcagg cgcgtcatac agttaccaca actggcgata
    79741 tttcccggtc cgccgacacg ggtaagaatg gtgttcagaa gttcactgct gatctctttg
    79801 gccatgggtt attccttgtc taaaacctga cgaataaagc cgccgtgttg atccaggcgt
    79861 tttttagctt ctgcggcatc gagatttttc agcaccatca caatggccgt tttacagttg
    79921 cgctcgcagg caattaacgc cgcttccgct tgttcggcat tacatccggt ggcgttttta
    79981 acgatattga cctggcgcac atgcagtttt tcgttggtgg cgaccacatc gaccatcagg
    80041 ttgccgaaca ctttgccgga tttaatcatc agcccggtgg agagcatatt gagcaccagt
    80101 ttctgcgctg tacctgcttt catccgcgaa gaaccggtaa ccacttcggc acctacaatc
    80161 ggtgtaatgg caaactctgc ggtggttgaa acggcgctcc ccggattaca ggaaattccc
    80221 actgtgcggc agccgagctg gcgtgcgtat tccagtccgg caattacata cggcgtgcga
    80281 ccactggccg caatgccaac caccacatcc tgcgcggtta agccgatgtt tttcagatcg
    80341 ttaacaccac cttcccggct atcttcagcg ccttccaccg catgctgaat ggcatattcg
    80401 cctccagcaa tcaaaccaac caccaaaccc ggtttcacgc cgtaggtggg cggacattcg
    80461 ctggcatcca gaatccccag acgaccggat gttcccgcac cgagataaat cagacgaccg
    80521 ccgccgctta cctgggcgtg gataacatcg atcgccgcgg cgatatccgg cagtacgcgc
    80581 tcaacggcaa gcggtacggt tttatcttca tcgttgataa tccggcacat ttccagcgtt
    80641 gatacgcggt caatttcagc cgaggcggcg ttcgagcctt cggtaatcat cttttcaagt
    80701 tgcatcggtg gtgaccttac tatttacgat caaataattg cagatgtatg gaatcatata
    80761 tcccgaattg acagacttaa aggaatcatt gagtctacac tcgcagagtg tgaagagcgt
    80821 cataagaact taagcggaat ttttgctgcc agcccaggag agtggaagat gttgtacctg
    80881 acgaaaatac gcaatgcgga aagtgaattt accgagaacg agcaaaaaat tgctgacttt
    80941 ctgcgggcca acgtgagtga gctgaaatcc gtctcttcgc gcaagatggc gaaacagctc
    81001 ggcatcagcc agtcgagtat tgtgaagttt gcccagaagc tcggcgcgca aggatttact
    81061 gaattgcgta tggcgttaat tggcgaatac agtgccagcc gggagaaaac gaatgccacg
    81121 gcgctgcacc tgcacagttc gattaccagt gatgattcgc tggaagtgat tgcccgcaaa
    81181 ctgaatcgcg aaaaagagct ggcgctggag caaacctgcg cgttgttcga ctacgcgcgg
    81241 ctgcaaaaaa tcattgatgt catcagtaaa gcgcaattta ttcagataac gggcctgggc
    81301 ggatcggcgc tggtggggcg cgatctgtcg ttcaaactga tgaaaattgg ctatcgcgtg
    81361 gcctgcgaag ccgacacgca cgtacaagcc accgtttccc aggcgttgaa aaaaggcgat
    81421 gtacaaatcg ctatctctta cagcggcagt aaaaaagaga ttgtcttgtg cgcagaagcg
    81481 gcacgaaagc agggggcaac ggtgattgcc attacttctc tggctgactc tccgctgcgg
    81541 cggctggcgc atttcacgct cgataccgtt tccggtgaaa ccgaatggcg tagctcgtcg
    81601 atgtctaccc gcacggcgca aaattctgtt accgatttac tgtttgttgg tcttgtgcaa
    81661 ctcaatgatg tcgagtcatt gaaaatgatt cagcgcagca gtgagctaac acaacgcctg
    81721 aaatgatgca taaagcagcg actggattga gattttcctg aattagtgag ctgatccgca
    81781 gcaatatttt gtttatcctg tattttcaga gggaatggag tgtaacgctc tgtattaaca
    81841 aggagagcat taaaatgggt aaactcacgg gcaagacagc actgattacg ggcgcattgc
    81901 agggaattgg cgaaggaatt gccagaactt ttgcgcgtca tggcgcaaac ctaatcttgc
    81961 tggatatctc ccctgagatc gaaaagctgg cggacgaact gtgtggtcgt ggtcatcgct
    82021 gtacggcggt tgtcgccgat gtgcgtgacc cggcgtcggt agccgcagct atcaaacgcg
    82081 cgaaggaaaa agaagggcgc attgatatcc tggtgaataa cgcaggcgtt tgtcgtctgg
    82141 gcagtttcct cgatatgagc gatgaagatc gcgatttcca tattgacatc aatattaaag
    82201 gcgtatggaa cgtcacgaag gcggtgctgc cagagatgat tgcgcgcaaa gatggtcgca
    82261 ttgtgatgat gtcttcagtc actggtgata tggtggccga tcctggcgaa acggcgtacg
    82321 ccttaacgaa agcggcgatt gttggcctga caaaatcgct ggcggtggag tacgcgcagt
    82381 ctggtattcg cgttaacgcc atttgcccgg gatacgtgcg cacaccaatg gcggaaagca
    82441 ttgcccgcca gtcgaacccg gaagatccag agtcggtgct gactgaaatg gcgaaagcaa
    82501 tcccgatgcg tcgcctcgcc gatccgctgg aagtcggcga actggcggcc ttcctcgcat
    82561 cggatgaatc cagctattta accggtacac agaatgtgat tgatggcggc agcacactgc
    82621 cggagacggt tagcgtcggt atctgattca cctctgtttc ctccctgcat ttgtggggag
    82681 gatttcgttt taaacccggt tcttattccc ttttcaactt ccaaatcacc aaacggtata
    82741 taaaaccgtt actcctttca cgtccgttat aaatatgatg gctattagaa agtcattaaa
    82801 tttataaggg tgcgcaatgg ccgttaactt actgaaaaag aactcactcg cgctggtcgc
    82861 ttctctgctg ctggcgggcc atgtacaggc aacggaactg ctgaacagtt cttatgacgt
    82921 ctcccgcgag ctgtttgccg ccctgaatcc gccgtttgag caacaatggg caaaagataa
    82981 cggcggcgac aaactgacga taaaacaatc tcatgccggg tcatcaaaac aggcgctggc
    83041 gattttacag ggcttaaaag ccgacgttgt cacttataac caggtgaccg acgtacaaat
    83101 cctgcacgat aaaggcaagc tgatcccggc cgactggcag tcgcgcctgc cgaataatag
    83161 ctcgccgttc tactccacca tgggcttcct ggtgcgtaag ggtaacccga agaatatcca
    83221 cgattggaac gacctggtgc gctccgacgt gaagctgatt ttcccgaacc cgaaaacgtc
    83281 gggtaacgcg cgttatacct atctggcggc atggggcgca gcggataaag ctgacggtgg
    83341 tgacaaaggc aaaaccgaac agtttatgac ccagttcctg aaaaacgttg aagtgttcga
    83401 taccggcggt cgtggcgcaa ccaccacttt tgccgagcgt ggcctgggcg atgtgctgat
    83461 tagctttgaa tcggaagtga acaacatccg taaacagtat gaagcacagg gctttgaagt
    83521 ggtgatcccg aaaaccaata ttctggcgga attcccggtg gcgtgggtcg ataaaaatgt
    83581 gcaggccaac ggtacggaaa aagccgcaaa agcctacctg aactggctct acagcccaca
    83641 ggcgcagacc atcatcaccg actattacta tcgtgtgaat aacccggaag tcatggacaa
    83701 actgaaaaac aaattcccgc agaccgagct gttccgcgtg gaagacaaat ttggctcctg
    83761 gccggaagtg atgaaaaccc acttcaccag cggcggcgag ttagacaagc tgttagcggc
    83821 ggggcgtaat taatgtttgc agtttcatca agacgcgtgc tgccgggctt taccttaagc
    83881 ctcggcacca gtctgctgtt tgtgtgcctg attttgctgc tgccgctctc cgcgctggtg
    83941 atgcagctgg ctcagatgag ctgggcgcag tactgggagg tgatcaccaa cccgcaggta
    84001 gtagcggcct acaaagtaac gctgctgtca gcgtttgtgg catcgatttt taacggcgtt
    84061 tttggcctgc tgatggcgtg gatcttaacc cgctatcgct ttccgggccg cacgctgctt
    84121 gatgcgctga tggatttacc ctttgcgctg ccaacggcag tcgccggttt aacgctagcc
    84181 tcgctctttt ctgtaaacgg tttttacggt gaatggctgg cgaagtttga tatcaaagtc
    84241 acctatacct ggctggggat tgcggtggcg atggccttta ccagcattcc gtttgtggta
    84301 cgtaccgtgc agccggtgct ggaagagtta ggcccggaat atgaagaagc ggcggaaacg
    84361 cttggtgcaa cgcgctggca gagtttctgc aaagtggtgc tgccggagct ttctccggcg
    84421 ctggtggcgg gcgtggcgct gtcgtttacc cgtagtcttg gtgaatttgg cgcggtgatt
    84481 tttatcgccg gaaatatcgc gtggaagacg gaagtgacgt cgctgatgat ttttgtgcgc
    84541 ttacaggagt ttgattaccc ggcagcgagc gcgattgctt cggtgatcct cgcggcatct
    84601 ctgctgctgc tgttctcaat taacactctg caaagtcgct ttggtcggcg tgtggtaggt
    84661 cattaatggc ggaagttacc caattgaagc gttatgacgc gcgcccgatt aactggggca
    84721 aatggtttct gattggcatc gggatgctgg tttcggcgtt catcctgctg gtgccgatga
    84781 tttacatctt cgtgcaggca ttcagcaagg ggctgatgcc ggttttacag aatctggccg
    84841 atccggacat gctgcacgcc atctggctga cggtgatgat cgcgctgatt gccgtaccgg
    84901 taaacctggt gttcggcatt ctgctggcct ggctggtgac gcgctttaac ttccctggac
    84961 gccagttact gctgacgcta ctggacattc cgtttgccgt atcgccggtg gttgccggtc
    85021 tggtgtatct gctgttctac ggctctaacg gcccgctggg cggctggctg gatgaacata
    85081 atctgcaaat tatgttctcc tggcccggaa tggtgctggt caccatcttt gtgacgtgtc
    85141 cgtttgtggt gcgcgagctg gtgccggtga tgttaagcca gggcagccag gaggatgaag
    85201 cggcgatttt gcttggcgcg tctggctggc agatgttccg tcgcgtcaca ttgccgaata
    85261 ttcgctgggc gctgctttat ggcgtggtac tgaccaacgc ccgcgcgatt ggcgagtttg
    85321 gcgcagtgtc ggtggtttcc ggctcgattc gcggcgaaac actttcgctg ccgttacaga
    85381 ttgaattgct ggagcaggac tacaacaccg tcggctcctt taccgctgcg gcactgttaa
    85441 cgctgatggc gattatcacc ctgtttttaa aaagtatgtt gcagtggcgc ctggagaatc
    85501 aggaaaaacg cgcacagcag gaggaacatc atgagcattg agattgccaa tattaagaag
    85561 tcgtttggtc gcacccaggt gctgaacgat atctcactgg atattccttc aggtcagatg
    85621 gtcgcgttgc tggggccgtc cggttccggg aaaaccacgc tgctgcgcat tatcgccggg
    85681 ctggagcatc aaaccagcgg gcatattcgc ttccacggca ccgacgtcag ccgcctgcac
    85741 gcgcgcgatc gcaaggtcgg tttcgtgttc cagcattacg cgctgttccg ccatatgacg
    85801 gtattcgaca atatcgcttt tggcctgacg gtgctgccgc gtcgcgagcg cccgaatgcc
    85861 gcagccatca aagcgaaagt gacaaaattg ctggaaatgg tccagcttgc gcatctggcg
    85921 gatcgttatc cggcgcagct ttccggcggc cagaaacagc gtgtggcgct ggcgcgcgcg
    85981 ctggctgtag aaccgcaaat tctgctgctt gatgaaccgt ttggcgcgct ggatgcgcag
    86041 gtgcgtaaag agctgcgtcg ctggctgcgt caactgcatg aagagctgaa attcaccagc
    86101 gtgtttgtga cccacgacca ggaagaggcg accgaagtag ccgatcgtgt agtggtgatg
    86161 agtcagggga atattgaaca ggctgatgcg ccgaatcagg tatggcgcga accagcgacc
    86221 cgttttgtac tcgaatttat gggcgaagtg aaccgcctgc agggaaccat tcgcggcggg
    86281 cagttccatg ttggcgcaca tcgctggccg ctgggctaca cacctgctta tcaggggccg
    86341 gtggatctct tcctgcgccc gtgggaagtg gatatcagcc gccgtaccag tctcgattcg
    86401 ccgctgccgg tacaggtact ggaagccagc ccgaaaggtc actacaccca attagtggtg
    86461 cagccgctgg ggtggtacaa cgaaccgctg acggtcgtga tgcatggcga cgatgccccg
    86521 cagcgtggcg agcgtttatt cgttggtctg caacatgcgc ggctgtataa cggcgacgag
    86581 cgtatcgaaa cccgcgatga ggaacttgct ctcgcacaaa gcgcctgata ggttgagtga
    86641 atgttaaacg cccggaggcg cttcccgcga tccgggcttt ttaatggcaa ggtttgtaac
    86701 ctgtagacct gataagacgc gcaagcgtcg catcaggcaa caccacgtat ggatagagat
    86761 cgtgagtaca ttagaacaaa caataggcaa tacgcctctg gtgaagttgc agcgaatggg
    86821 gccggataac ggcagtgaag tgtggttaaa actggaaggc aataacccgg caggttcggt
    86881 gaaagatcgt gcggcacttt cgatgatcgt cgaggcggaa aagcgcgggg aaattaaacc
    86941 gggtgatgtc ttaatcgaag ccaccagtgg taacaccggc attgcgctgg caatgattgc
    87001 cgcgctgaaa ggctatcgca tgaaattgct gatgcccgac aacatgagcc aggaacgccg
    87061 tgcggcgatg cgtgcttatg gtgcggaact gattcttgtc accaaagagc agggcatgga
    87121 aggtgcgcgc gatctggcgc tggagatggc gaatcgtggc gaaggaaagc tgctcgatca
    87181 gtttaataat cccgataacc cttacgccca ttacaccact accgggccgg aaatctggca
    87241 gcaaaccggc gggcgcatta cccattttgt ctccagcatg ggaacaaccg gcactatcac
    87301 cggcgtttca cgctttatgc gcgaacaatc caaaccagta acgattgttg gcctgcagcc
    87361 ggaagaaggc agcagtattc ccggcattcg ccgctggcct gcggaatatc tgccggggat
    87421 tttcaacgct tctctggtgg atgaggtgct ggatattcat cagcgcgatg cagaaaacac
    87481 catgcgcgaa ctggcagtgc gggaaggaat attctgtggc gtcagctccg gcggcgcggt
    87541 tgccggagca ctgcgggtgg caaaagctaa ccctggtgcg gtggtggtag cgatcatctg
    87601 cgatcgtggc gatcgctacc tttccaccgg ggtgtttggg gaagagcatt ttagccaggg
    87661 ggcggggatt taagattatt gcgcaggcac cgttttatcc gtctgcgcaa ttagcgcatg
    87721 taaaaaaggc tgtgcgttct catcgctctt accggggatc ttcacaatat acggcttccc
    87781 ggtaatcgat gacgacgaag caaccttatc aataaactgc tcggcagtgt caatgcggtt
    87841 acgggtattg cccagcttca gacgcagatg agaaaccgct tcatcgcagg tatgttcatc
    87901 accgttgcgc acaaatatca aatccttttt ctgtgctaat ccctccagca tggcgttgat
    87961 gcgggcttct tcatgggcag ttaacttcgc gtaagcgggg agtgtcatta gtagtgtaat
    88021 gaccagacaa atgattttct tcattgaatt accttatcct tgttttgtga tagccgattt
    88081 gactgccaga atcaggtaaa gtttcggttg gaatacgatg aggatcatcg gcaaggagaa
    88141 aaaattaaat gagtagtttg ttgttgttta acgataagag cagggcgctg caggcggata
    88201 tcgtcgccgt gcagtcgcag gtggtttacg gcagcgtggg caacagcatt gccgtgcctg
    88261 ctatcaaaca gaacggtctg aatgtctttg ccgtgccgac ggtattgctg agcaatacgc
    88321 cgcattatga cactttctac ggtggtgcga ttccggacga atggtttagc ggctatttgc
    88381 gtgcgcttca ggagcgtgat gcgctgcgcc aacttcgtgc tgtaaccacg ggctatatgg
    88441 gaacggcatc gcaaatcaaa atccttgccg agtggctgac tgcgctacgc aaagaccatc
    88501 ctgacctatt gatcatggtc gatccggtga ttggcgatat tgatagcgga atttatgtca
    88561 aacctgacct tcccgaggcg tatcgacaat atttactgcc gctggcgcag ggaattaccc
    88621 ccaatatctt tgagctggaa atcctgaccg gtaaaaattg ccgcgatctc gacagtgcca
    88681 ttgctgccgc aaaaagtctg ctttcagaca cattaaaatg ggtggtgatt accagcgcct
    88741 ccggtaatga agaaaatcag gagatgcagg tggtggtggt cagtgccgac agcgtgaatg
    88801 tcatttccca ttcacgggta aaaaccgacc tgaaagggac tggcgacctg ttttgtgctc
    88861 agcttatcag tggtttgctg aaagggaagg cgttaaacga tgcggtgcac cgtgcggggt
    88921 tgcgcgtact ggaagtgatg cgctacaccc agcagcatga gagcgatgaa ttgattttgc
    88981 cgccgctggc ggaagcataa aaaatggcgc cgatgggcgc catttttcac tgcggcaaga
    89041 attacttctt gatgcggata accggggttt cacccacggt tacgctaccg gacagtttga
    89101 tcagttcttt gatttcgtcc atgttggaga taacaaccgg agtcagggta gacttggctt
    89161 tctcttccag cagcggcaga tcaaattcaa tgacagtatc gccaactttc acgcgctgac
    89221 cttcttcagc aatacgcttg aagccttcgc ctttcagttc aacggtgtcg ataccgaagt
    89281 ggacgaacag ttcaacgccg ctatcagatt cgatagagaa cgcgtggttg gtttcaaaga
    89341 ttttaccaat ggtgccgtct acaggcgcga ccattttgtt acccgttggt ttgatagcaa
    89401 taccatcacc aacgattttt tccgcaaaaa cgacatccgg cacgtcttcg atattgacga
    89461 tctcgccaga gagcggagca atgatctcaa tagttccggt atccttcttg tcgtcggaaa
    89521 ccagagattt cagtttatcg aacaaaccca tgatcttctc ctaagcagta aattgggccg
    89581 catctcgtgg attagcagat tgttttttct tcaatgaact tgttaaccag cgtcattaac
    89641 tcgtccgttg tcggttgagc aagagcctgc tctgctaaca ccttcgcatc ttcgaagttc
    89701 gtgttacgga taatcttctt aatgcgcggg atagaaatgg cgctcataga gaattcgtcc
    89761 agacccatcc ccagcaacag aagtgtagca cgttcatcgc cagcaagctc accacacatg
    89821 ccagtccatt tgccttcagc atgagaagca tcaataactt gcttgatcaa gttcagcacg
    89881 gacggtgaca ttggttggta aaggtgtgaa atcatatcat taccacggtc aactgccaga
    89941 gtgtactgcg ttaaatcatt ggtgccgata ctaaagaaat caacttcttt ggctaaatga
    90001 cgtgcaattg ttgcggcagc cggtgtttcc accattacgc cgatttcaat tgactcgtca
    90061 aacgctttac cttcgtcgcg cagttcctgt ttgtagattt cgatctcttt gcgcagtgca
    90121 cgcacttctt caacagagat gatcatcggg aacataatgc gcaatttacc gaaagccgag
    90181 gcacgcagga tagcgcggag ctgatcgcgc aggatctctt tacgatccat cgcgatacgg
    90241 atagcgcgcc agccgaggaa cgggttctct tctttcggga agttcatgta tggcagctct
    90301 ttgtcgccgc cgatgtccat ggtacgaacg ataaccgcct gcgagccaca cgcttcagcc
    90361 actgctttgt aagcagcaaa ctgttcttct tcagtgggca gcgcgtcgcg gtccatgaac
    90421 aggaactcag tacgatacag accaacgcct tcagcgccgt tacgctctgc accttcaacg
    90481 tcacgaaccg taccaatgtt agcgcacact tctacctggt gaccgtccag cgtaatagct
    90541 ggcagatctt tcagtttagc aagctctgct ttttcagaag ccacttgctc ctgaacagcg
    90601 cgcattttat caataacttc gttggttgga ttgacgtaaa cctgattatt tacggcatcc
    90661 agaatcagat agtcgtcatt tttcacctga gaggtgacgc taccggtacc cacgatagca
    90721 ggcagttcca gagaacgcgc catgatagag gtgtgggaag tacggccacc cgcgtcggtg
    90781 atgaaaccca gcaccttctt caggttcagc tgtgcggttt cggacggcgt caggtcagcg
    90841 gcaaccagaa tgacttcatc ctgaatggcg ctcaggtcga taatcttcag gcccaggatg
    90901 ttgcgcagca ggcgcttacc gatatcacgt acgtcagccg cacgttcttt caggtattca
    90961 tcatccagct cttccagggc agaagcctga ccttcgataa cttcatgagc agctgcgtca
    91021 gctgtcatgt gcttatcttt aatcagggct atgatttcct gctccagctc ctcatcttcg
    91081 agcagcataa tatgcccttc aaagatggct tctttttctt caccgaacgt ttcaccagct
    91141 ttcgttttga tcgtttccag ctgggctgat gccttggcac gaccgctcag aaaacgttca
    91201 acttcctgat caacctggtc ggcagaaatt tttttccggt caatgacaat ttcgtcttct
    91261 ttcagaagca gagctttacc gaaagcgata cccggggatg ctaaaatgcc tgaaatcata
    91321 accctacctt acttgtgact gatttttaaa agaacccggg aaattactcg agttccgcca
    91381 tcagtttaac cagatgttca accgctttct gctcgtcttc gccttctgcg gagatagtca
    91441 caacggtacc ttgagtcagg cccagagtct gcagtttaaa caggcttttc gcgctggcgc
    91501 ttttgccgtt ggaagtcaca gtaatttcag aagtgaagcc cttagcttct tttacaaact
    91561 gggcagcagg gcgggtgtgc agaccgttcg gagcggtaat ggtaacttct tgctggaaca
    91621 ttgtatttcc ccaacttata ggtttagtgt tgtggaacta aagtctagcc tggcggttcg
    91681 actttagcct gtattgttag cgccagtttt taacagacgc gacgcacgaa gagcgcaata
    91741 cagtccattg ctggcgggtg ttgaccgctg acgtttcgta cgtcattaaa cattatgccg
    91801 ctaaaggaag aattgaacca aatcataaaa tcgattcagc tttggctttt cctgtaacga
    91861 ttaatttcgc gcatcaaaat aattagtctg gttaaatacc agttccgaca ggctgaatca
    91921 atgccaggag gggcgaaagt ttgaagcagg ccacaaaaaa gcacctaaaa aggtgctttt
    91981 ttacgcattt ttaacaagct ggcattactg ttgcagttct ttctcagtga agagatcggc
    92041 aaacaatgcg gtgcttaaat aacgctcacc cgatgacggt agaataacca caatattctt
    92101 gttggtaaag ctttcatctt cttgtagttt caacgccgca gcaacagctg ctccagaaga
    92161 gatacctgca agaatacctt cttcttccat cagacgacgt gcggtagaaa tcgcttcttc
    92221 attggtgatg ccaatgactt tatcgaccag cttgagatcg aggttagctg ggataaaacc
    92281 agcgccaata ccctgaattt tatgcgggcc aggtttaatc tcttcaccag ccagtgcctg
    92341 ggcgataact ggagaatcgg ttggctcaac ggcgacagag ataagatcgg tcttgccttt
    92401 ggtgccttta atgtagcggc tgacgccagt cagcgtaccg ccagtcccaa cgccagcaat
    92461 aaatacatca acctgaccgt cggtatcttc ccagatctcc ggaccggtgg ttttttcgtg
    92521 aatttcaggg tttgccggat tgctgaattg ttgcagcagc aggtatttct ctggattgct
    92581 ggcgacaatt tcttctgctt tttggattgc gcctttcatg cctttagcac cttccgtcag
    92641 caccaggttt gcacctaacg ctttcagcag cttgcggcgt tcaatactca tggtttctgg
    92701 catggtcagg gtgagtttgt aaccgcgagc ggcagctaca taggccagtg caatcccggt
    92761 attaccgctg gtcggttcaa ccagttcaac gcctggtttc agcacgccgc gcttttcggc
    92821 atcccaaatc atgttggcac cgatacggca cttaacgctg aagctggggt tacgagattc
    92881 caccttcgcc agaatgcgtc cgttaccgat gcgattcagg cgaaccagcg gcgtgtgacc
    92941 gatagtcagc gagttatctt caaaaatctt actcatggcc tgtccttaac tgtatgaaat
    93001 tgggatacaa caggtagcat acccgctcag agaatatgcg gaagtaagga tttagcatat
    93061 ctatatacag aagggaaata atgacatgca agatggaata aggggcggca taagccaccc
    93121 ctgtttcaca caaacggttt acaaaataac cggtagattg ttaccgccac attgcgtgtt
    93181 tatcgcgata gcaatcgacc cacatcgccg tcgcgccaca aacggcaacg ggcatgatga
    93241 acagattaag cagcgggatc atcgtaaaca ggctggttaa agcaccaaac tgcatattgg
    93301 tgattttgcg tgtgcgcagg gcggtgcgca tctctttaaa cggcactttg tggttatcga
    93361 aggggtaatc gcaatactgg atggctaaca tccaggcgct aaacaggaac cacagtaccg
    93421 gcgcgacggt ttgcccaata ccagggatta agtaaagaat tagcaataca attgcgcgcg
    93481 gcagatacca ggcaaatttt tgccattcgc gtttcatgat tcgcggcaca tctttcatga
    93541 taccgaaaat cccggtatct ggcggtgtag cgccagtcaa tcgtgcttcc agttgttcag
    93601 ccaataaacc gttaaacgga gcggcaatcc agttagcaat cgtggagaag aaatagccaa
    93661 acactaacag cacagagatg acagccagag gccacaacag ataactcagc cattgtagcc
    93721 agtccggaac gtaactcatg agagtcggga tccagacatc gagctgtgta aagagccacc
    93781 agaatgcgcc ccccatcaac aaaatattga ccagcagcgg taaaataacg aaacgccgaa
    93841 tcccaggttg cgagacgagc ttccagcctt gcgcaaaata gtaaaaaccg ctgcgtgggg
    93901 cagatgtgaa tgatgaaacc ataatcagga tgagctcctt ttgaccaatc ccaggaaaat
    93961 tctgcgtatt ttaccgggta attgcgcaat ggacagttag gatatgttcg aaaaaacagc
    94021 aaaaagcacg atttcatcta tctttgtgct gtgaaagtta atagtgcact tgcacttgag
    94081 gtaatcggca aatactctta gtgagtaaat gtttgccgtg gtggcaaggt gttagaacaa
    94141 cagagaatat aatgatgcag gatttgcgtc tgatattaat cattgttggc gcgatcgcca
    94201 taatcgcttt actggtacat ggtttctgga ccagccgtaa agaacgatct tctatgttcc
    94261 gcgatcggcc attaaaacga atgaagtcaa aacgtgacga cgattcttat gacgaggatg
    94321 tcgaagatga tgagggcgtt ggtgaggttc gtgttcaccg cgtgaatcat gccccggcta
    94381 acgctcagga gcatgaggct gctcgtccgt cgccgcaaca ccagtaccaa ccgccttatg
    94441 cgtctgcgca gccgcgtcaa ccggtccagc agccgcctga agcgcaggta ccgccgcaac
    94501 atgctccgcg tccagcgcag ccggtgcagc agcctgccta tcagccgcag cctgaacagc
    94561 cgttgcagca gccagtttcg ccacaggtcg cgccagcgcc gcagcctgtg cattcagcac
    94621 cgcaaccggc acaacaggct ttccagcctg cagaacccgt agcggcacca cagcctgagc
    94681 ctgtagcgga accggctcca gttatggata aaccgaagcg caaagaagcg gtgattatca
    94741 tgaacgtcgc ggcgcatcac ggtagcgagc taaacggtga actgcttctt aacagcattc
    94801 aacaagcggg cttcattttt ggcgatatga atatttacca tcgtcatctt agcccggatg
    94861 gtagcggccc ggcgttattc agcctggcga atatggtgaa accgggaacc tttgatcctg
    94921 aaatgaagga tttcactact ccgggtgtca ccatctttat gcaggtaccg tcttacggtg
    94981 acgagctgca gaacttcaag ctgatgctgc aatctgcgca gcatattgcc gatgaagtgg
    95041 gcggtgtcgt gcttgacgat cagcgccgta tgatgactcc gcagaaattg cgcgagtacc
    95101 aggacatcat ccgcgaagtc aaagacgcca acgcctgata cacttaaggc aaattaactc
    95161 ctcttcgaac ccccgcttgt cgggggtttt tagcattgat ggtgcgatat ggaatcaatc
    95221 gaacaacaac tgacagaact gcgaacgacg cttcgccatc atgaatatct ttatcatgtg
    95281 atggatgcgc cggaaattcc cgacgctgaa tacgacaggc tgatgcgcga actgcgcgag
    95341 ctggaaacca aacatccaga actgattacg cctgattcgc ctacccaacg tgtaggcgct
    95401 gcgccgctgg cggctttcag ccagatacgc catgaagtac caatgctgtc actggataac
    95461 gtttttgatg aagaaagctt tcttgctttc aacaaacgtg tgcaggaccg tctgaaaaac
    95521 aacgagaaag tcacctggtg ctgtgagctg aagctggatg gccttgccgt cagtattctg
    95581 tatgaaaatg gcgttttagt cagtgccgcg acccgtggcg atggcaccac cggggaagat
    95641 atcacgtcta atgtgcgtac tattcgcgct attccgctga agctgcacgg agagaatatc
    95701 ccggcgcgtc tggaagtgcg tggtgaagtg ttcctgccgc aggcggggtt cgaaaagatt
    95761 aacgaagatg cgcgacgcac gggcgggaaa gtttttgcta acccacgtaa tgcggcagct
    95821 ggttcactgc gtcagcttga tccgcgtatt acagcgaagc gaccgctcac ttttttctgc
    95881 tatggcgttg gtgttctgga aggtggcgag ctgccggata ctcatcttgg ccgtttactg
    95941 caatttaaaa agtgggggtt gccggtcagc gatcgggtaa cgctttgtga atcggcggaa
    96001 gaagtgctgg cgttctatca caaagtggaa gaagaccgcc cgacgctggg ctttgatatc
    96061 gacggcgtgg tgattaaggt caactcactg gaacagcagg agcagcttgg ctttgtcgcg
    96121 cgtgccccgc gctgggcggt agcgtttaaa ttcccggcgc aggagcagat gacctttgtg
    96181 cgtgacgtcg agtttcaggt tgggcgtact ggcgcgatta cgcctgttgc gcgtctggaa
    96241 cctgtccatg ttgctggcgt gctggtgagt aacgcaacct tacacaatgc ggatgaaatc
    96301 gaacgtcttg gtttacgcat tggcgataaa gtggtgattc gccgcgctgg cgacgtgatc
    96361 ccgcaggtgg ttaacgtcgt gctttctgaa cgcccggaag atacccgtga ggttgtattc
    96421 ccgacgcatt gtccggtatg tggttctgac gttgagcgtg tggaaggtga agcggttgcc
    96481 cgctgtaccg gtggcctgat ttgcggagcg cagcgtaaag agtcgctgaa acactttgtt
    96541 tcccgccgtg cgatggatgt tgacggaatg ggcgacaaaa tcatcgatca gctggttgaa
    96601 aaagaatatg tccatactcc ggcagatctg ttcaaactca ccgcaggcaa actgaccgga
    96661 ctggagcgta tggggccaaa atcggcacaa aacgtggtta acgcgctaga aaaagcgaaa
    96721 gaaaccacct ttgctcgctt cctctatgca cttggcatcc gtgaagtcgg cgaggccacc
    96781 gcagcaggtc tggcggcata tttcggcacg ctggaagcgc tggaagccgc ttcgattgaa
    96841 gagctgcaaa aggtgcctga tgttggcatt gtcgttgcat cccacgttca caacttcttt
    96901 gccgaagaaa gcaaccgcaa tgtcatcagc gagctgttgg cggaaggtgt tcactggcct
    96961 gcgccgatcg ttatcaacgc ggaagagatt gacagcccgt ttgctggtaa aaccgtggtg
    97021 cttacgggca gcttaagtca gatgtcacgt gatgacgcta aagctcgact ggtcgaactg
    97081 ggcgcgaaag tcgcgggcag cgtgtcgaag aaaaccgatc tggtgatagc gggtgaagct
    97141 gcaggatcta aactggcgaa ggcgcaggaa ctgggcattg aagtcatcga cgaagcggaa
    97201 atgctgcgtt tgctgggtag ctgagatgga aaaagagcag ctgattgaaa tagccaatac
    97261 gataatgccg tttggcaaat acaaagggcg tcgcttaatc gacctgccgg aggtgtatct
    97321 gctgtggttt gcccgcaaag atgagttccc ggcagggaag ctcggtgagc taatgcaaat
    97381 cacgctgctg attaaaaccg aggggctgac gcaactggtc cagcccctga agcgtccgct
    97441 ttaagcttta tcggtgctgc tttcctgctg cgcctgtaac tgttcggtct ggcgtttgta
    97501 tcgacgcgcc agcaccgcac agaccatcaa ttggatctga tggaaaatca tcaggggcag
    97561 caccatcata ccgatcaccg atgtggggaa cagaatgttt gccatcggga tgccatttgc
    97621 cagactcttt ttcgaaccac aaaagacgat agtaatttca tctgccttat tgaagcccag
    97681 tcggcgtgcc ataaagacgt taactacaat cacgatagcc agaagaacgc agctgaccac
    97741 cacgataaac agcaatgatc cccagccaac tttatgccag ataccattaa cgacggcttc
    97801 gctgaacgct gtataaacca ccaacagaat ggacgtctgg tcagttttcg caatccattt
    97861 tttattgcgc gacacccagt caccaatcca cggccgggaa agatgcccca acacaaacgg
    97921 cagcagcagt tgcagcataa ttttaccgac ctgctcaagg ctgccccctg caccgtgaac
    97981 attcatcacc agaccaacca gcaatggtga aaggaaaatc cccagcaggc tggatgctga
    98041 cgcagaacaa accgccgccg cgacgttacc gcccgccatt gacgtgaagg cgattgcaga
    98101 ctgcacggta gccgggagaa tgcacaagta gagaaaaccg gagtagagca tcgggtcgac
    98161 atttaccggt ttccaccagg caaacagtac acccagaatc ggaaacagca cgaaggtgct
    98221 gcaaattacc cacaaatgca gtcgccagtg accaccgcca gcaataatcg cctcacgcga
    98281 caacttcgcg ccgtgcataa agaacagcag ggcaatcgct gcggtggtca gattttcaaa
    98341 gaaggggacg aaatcgcctc tggccggaaa gaaagaggcc agcaacacca ccgtgatcag
    98401 ggttaaggtg aaaggatcga ggatacgaaa aagtttcata aagactccag atgatcgatg
    98461 gttctattgt gcttcttcca gtttgagaaa taaaattgat ttattgcatc cattcatgaa
    98521 gaaaacagat gaattattct ttaaaacaat taaaggtttt cgtcacagta gcgcaggaga
    98581 aaagttttag tcgtgcagga gagcgtatcg gcctgagcca gtcggcagtg agtcacagtg
    98641 tgaaggaact ggaaaatcat actggtgttc gcctgctgga cagaaccacg cgtgaagtgg
    98701 tgcttacaga tgcagggcag cagctggctt tgcgtcttga gcgactgctg gatgaactga
    98761 acagcacgtt gcgcgatacc gggcgtatgg ggcaacaact gagcggaaaa gttcgggtcg
    98821 ctgccagcca gaccatttcc gcgcatctta ttccgcaatg cattgctgaa agccatcgcc
    98881 gctatccaga tattcagttt gtcctgcacg atcgtccgca gcagtgggtg atggaaagta
    98941 ttcgccaggg agatgtcgac tttggcatcg tcatcgatcc aggcccagtg ggcgatctgc
    99001 aatgtgaagc gattctttcc gagcctttct ttctgctttg ccatcgcgat agcgctttgg
    99061 ccgtcgaaga ttatgtaccc tggcaggcat tacagggagc gaaattggtg ttgcaggatt
    99121 acgcgtcagg cagtcgaccg ctgattgacg cagcgctggc gcgcaatggt attcaggcga
    99181 atattgtgca ggagattgga catccggcga cgctgttccc gatggtagcc gcaggcatcg
    99241 gcatcagtat tcttcccgca ctggcgctac cattgcctga aggtagccca ctggtggtaa
    99301 agcgcattac gccggttgtt gaacgccagt tgatgctggt gcgcaggaag aaccgttcac
    99361 tttccaccgc cgctgaagcg ttatgggacg tggtacgaga ccagggcaat gcgctaatgg
    99421 ctggtcggga aggggatccg ttgtatcaga tatagagtga attcatactg ccgggagccc
    99481 cggcagtaat caatcagaag ttaaatttga tggagtagat tatcgcatcc tggtaaaaat
    99541 cttcgcccag tttgtcatcc gcatatcgat actgaatccc cgtagtaatg tgtgatgttg
    99601 cattccacca cagtgcaact gcgccattca gcccttcttt accgccatta cccgctgcat
    99661 aggtagcgtc acggtcaaac tcgtactcgt tccagttggt aagagagaat ttctcgctgc
    99721 ccagcataaa gttgtaacct gcaacccagc cggcgacata gccgttgtcg ccagtgtaat
    99781 aggtttgatc tgtataacgc ttagcaaaga acggtttgaa ccaccaaccg ctgccggtga
    99841 aattgtagcc gataccgtac aggaacatat cgtcgtggaa attcacgcga ttcgcagaac
    99901 cgtaggtgcc atacgcatgc aagtagagat taaatccggt gtcacccagg taaatacggt
    99961 tggtattttt aaaggtataa cgctgctcgc tacctggttt attatggcga ccgttataga
   100021 agttttccca gtcgaagaac ccgtacattt ctccccaact aaagttagcg ccaccttcaa
   100081 attcaagata accaaaatca tctttgtgtg atttggtcga cgatttttcg gtagtccggc
   100141 tggtccagtc cagataatgc acgccgatat ctgcaaaacc gcctttaaat tctgcatgag
   100201 atacaacagg tatcgctaat atagaggaaa gtgtcagagt taaaagatgt tttttcatag
   100261 cataattccc tatgccgatc gttattagta gattgcttaa taaagtggtt tgctaaaaac
   100321 gacatattgt taatgcaaag gaattaatat cgccaataca gtttttaccg tggattaata
   100381 gagaaaagat ataaatccat cgatatcgaa tcgctattga atgcgtgaga aaagtgtatt
   100441 ggtaagagcc gggacaacct cgctataaaa aggcgttaga ttccacccta cagaaaaagg
   100501 atatgtatgt ctcaggttca attttctcat aacccgctgt tttgcataga tattatcaag
   100561 acttataaac ctgatttcac gccacgagtg gcctttattt taggttccgg gctcggcgcg
   100621 ctggccgatc agattgagaa cgctgtcgca atttcctacg aaaagctgcc gggcttcccg
   100681 gtaagtacgg tacatgatca tgcgggggag ttagtgctgg gccatctcca gggcgtaccg
   100741 gtggtatgta tgaaaggtcg cggacatttc tacgaaggtc gtggaatgac cattatgact
   100801 gacgcaattc gtacctttaa gctgctgggc tgcgagctac tgttctgcac caatgcggca
   100861 ggctcgctgc gtccggaagt gggggcaggc agcctggtcg cattgaaaga tcatatcaac
   100921 accatgcccg gtacgccgat ggtgggtctt aacgatgatc gttttggaga gcgcttcttc
   100981 tcactggcaa atgcctacga tgcggaatac cgcgcactgt tacaaaaagt ggcgaaagaa
   101041 gagggtttcc ctctgacgga gggcgtgttc gtctcgtatc cggggccgaa tttcgagact
   101101 gcggcggaaa ttcgcatgat gcaaattatt ggtggggatg ttgttggtat gtctgtggtg
   101161 cctgaggtta tttcagctcg ccattgcgac cttaaagtcg ttgcggtctc tgcgattacc
   101221 aatatggcgg aaggtttaag cgatgtgaaa ttgtcacatg cgcaaacgct ggcggctgcg
   101281 gagctctcta agcagaactt catcaacctt atttgtggct ttctgcgcaa aattgcctga
   101341 gaaaaacaat aagaaacggc ctcgcgggtg aggccaaatg atgcgacaag gaaacgatta
   101401 tgagcatcgc gatgcgctta aaggtaatgt cctttttgca atattttatc tgggggagct
   101461 ggctggttac cctcggctct tacatgatta atacccttca tttcaccggc gctaatgttg
   101521 gcatggttta cagttccaaa gggatcgccg cgattattat gcctggtata atggggatca
   101581 tcgcagacaa atggctgcgc gcagaacgtg catacatgct gtgtcacctg gtgtgtgcgg
   101641 gcgtactttt ttatgcggca tccgtaactg atccggatat gatgttttgg gtgatgttag
   101701 tcaatgcgat ggcgtttatg ccgactattg cgttatcgaa cagcgtctct tattcctgtc
   101761 ttgcccaggc agggcttgac ccggtgaccg ctttcccgcc cattcgcgtt tttggtacgg
   101821 tggggttcat tgtcgcgatg tgggcagtaa gcctgctgca tctggaattg agtagtctgc
   101881 agctgtatat cgcgtccggt gcgtcattgc tgctgtcggc ttatgcgctg actttgccga
   101941 agattccggt tgcggagaaa aaagcgacca catcgcttgc cagcaagctg ggtctggatg
   102001 ccttcgtgct gtttaaaaat ccacgcatgg ccatcttttt cctctttgcc atgatgctgg
   102061 gtgcggtact gcaaattacc aacgtttttg gtaatccgtt cctacatgat ttcgcccgta
   102121 acccggagtt tgctgacagt tttgtggtga aatatccctc cattttactg tcagtttcac
   102181 agatggcaga agtgggcttt atactgacta tcccattctt tttaaagcga tttggcatta
   102241 aaaccgtcat gctgatgagt atggtggcct ggacgctgcg ctttggcttc ttcgcctatg
   102301 gcgatccgtc aacaaccgga tttattttgc tgctgctgtc gatgattgtt tatggctgtg
   102361 cattcgattt cttcaatatt tctggttcgg tatttgtcga acaggaagtt gattccagca
   102421 ttcgtgccag cgcgcagggg ctctttatga ccatggtaaa tggtgtcggc gcatgggttg
   102481 gctcgattct gagtggcatg gcagtagatt acttttcggt ggatggcgta aaagactggc
   102541 aaactatctg gctggtgttt gcaggatatg ctctttttct cgcagtgata tttttctttg
   102601 ggtttaaata taatcatgac cctgaaaaga taaagcatcg agcggtggct cattaaaagg
   102661 attcgcagct ctgctcttca gagctgcttt tatgataaag gttaattagt gaaagatatt
   102721 ttattttctt ccggcgttgg ttttggcatt ggtgctttgt ttacaatcgt gcgcttgcct
   102781 attcctgtac caaatgtatt accaggtata ttatccattg tgtttatgta tgtcggatat
   102841 ctggtggtga aatactttat gcaatgataa tttaatacga tgtatttatt atatggagca
   102901 cttaattatg gaacgcgtat acagaacaga tcttaagttg ctccgttatt ttcttgccgt
   102961 agcggaagag ttgcattttg gccgcgcagc agcgcgttta aatatgtctc agcctccgct
   103021 caggattcat attaaagagc tggaaaatca actcggcacg cagcttttta ttcgccattc
   103081 gcgcagcgtc gtactgacac acgcgggcaa aatcttgatg gaagaatcgc gtcgattgct
   103141 ggtgaatgct aataatgtat tggctcgggt tgaacaaata ggtcggggag aagcagggcg
   103201 gattgaactc ggcgtagtgg gaacggctat gtggggacgg atgcgcccgg ttatgcggcg
   103261 attcctcagg gaaaatccta acgttgaagt tctttttcgc gaaaagatgc ccgcgatgca
   103321 aatggccctg ctggaacgac gtgaactcga tgccgggatc tggcgaatgg cgacagaacc
   103381 accgacaggt tttaccagct tacggttgca tgaatcggcg tttctggtgg cgatgcctga
   103441 agagcatcat ctctcatcat tttccaccgt tccgctggaa gcgctacgtg acgagtattt
   103501 tgttacaatg ccgcccgtgt acactgactg ggattttttg cagcgagttt gccagcaggt
   103561 gggattttca ccggttgtta ttcgcgaagt taatgaaccg caaacggtac tcgccatggt
   103621 cagtatgggt attggtatca cattgatagc ggacagctac gcacaaatga actggccagg
   103681 tgtcattttc cgtccgctca aacagcgcat ccctgcagat ttatatattg tttatgaaac
   103741 acagcaggtg acgcccgcga tggttaagct gttggctgca ttgacacagt aggtgaggag
   103801 acaggaattg ccggaaatag tcgtctgcta agtgaagcgc atcaggcaat ttcgcaaatc
   103861 gcagatagca aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg tgcaggattc
   103921 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccgaag
   103981 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
   104041 gctgagctaa tcacccactt cggtacttca catttttaca agaaatttag ctggcgagaa
   104101 agtggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc
   104161 cagctgagct aatcacccac ttctcaattt cttgctacac ggcggagact gcataaagta
   104221 gttggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc
   104281 cagctgagct aatcaccccc gctgtgtgga gtcgcattat agggagagtt caaaatgagt
   104341 caacgcattt tctaaagaaa ttgttcgttc gtcgtaaatt taagcaagat gatcgcaaaa
   104401 cagaccgtgt tgcgcaattt gtcaacgaaa acaataatgc gtaaggtaga aacccgaact
   104461 acattgagga atcaggcggg agtgatagaa tatcgcccac tcaatttttc caggatttgc
   104521 cggttgtcgg catctttcta aacgtaaggc catttcatga aaatcaaaac tcgcttcgcg
   104581 ccaagcccaa caggctatct gcacgttggc ggcgcgcgta ctgctcttta ctcctggctt
   104641 tttgcacgta accacggcgg tgagttcgtg ctgcgtattg aagacaccga tcttgagcgt
   104701 tccacgccgg aagctatcga agccattatg gatggcatga actggctgag cctggagtgg
   104761 gatgaaggtc cgtactacca gaccaaacgt tttgatcgct acaatgcggt gatcgatcag
   104821 atgctggaag agggcactgc ttataaatgc tattgctcta aagagcgcct ggaagcgctg
   104881 cgcgaagagc aaatggcgaa aggtgagaag ccgcgttatg acggtcgctg ccgccacagc
   104941 catgagcatc atgctgatga tgaaccgtgt gtcgtgcgtt ttgctaatcc gcaggaaggt
   105001 tctgttgttt ttgacgatca gatccgtggt ccgatcgagt tcagcaacca ggagctggat
   105061 gatctgatta tccgccgtac cgatggttcc ccaacctata acttctgtgt ggttgtcgat
   105121 gactgggata tggaaatcac ccacgttatc cgtggcgaag accatatcaa caacacgccg
   105181 cgccagatca acattcttaa ggccctgaaa gcgccgctgc cggtttacgc gcacgtttct
   105241 atgatcaatg gcgatgacgg taaaaaactg tccaaacgtc acggggcagt cagcgtaatg
   105301 cagtatcgtg atgacggtta tttgccagaa gcactgctga actatctggt gcgtctgggc
   105361 tggtcccacg gcgatcagga aatcttcact cgtgaagaga tgatcaaata cttcactttg
   105421 aatgccgtca gcaaatctgc cagtgcgttc aacaccgaca agctgctgtg gctgaaccat
   105481 cactacatta acgcgctgcc gccggagtat gttgctactc acttacagtg gcacattgag
   105541 caggaaaata tcgatacccg taacggcccg cagctggctg atctggtgaa actgctgggc
   105601 gaacgctgca agacgctgaa agagatggca cagagctgcc gttatttcta cgaagatttt
   105661 gctgagttcg atgccgatgc ggcgaaaaaa catctgcgtc cggtagcgcg tcagccgctg
   105721 gaagtggttc gtgacaaact ggccgcgatt actgactgga ccgctgaaaa cgttcatcac
   105781 gctattcagg cgacggcgga tgagctggaa gtgggtatgg gtaaagttgg tatgccgctg
   105841 cgtgtcgccg taaccggtgc ggggcagtct ccagcactgg atgttaccgt tcacgcaatt
   105901 ggtaagaccc gcagtatcga gcgtatcaac aaagcgctgg attttattgc tgaacgcgaa
   105961 aatcagcagt aattagcgtg taaaaagata aacggcagga gataatatcc tgccgttttt
   106021 tatttatgct gattcgtcaa tatcgagacg ggcgaggaaa ttgcgaattc cttcacgtga
   106081 caaaaattgc gcgacttttt cctgctcaat gtctgtcata ttatctatcg cgttgatgat
   106141 ttgtcgatac gccggggcgc gtgctccagg agcgtattca gcaattctct cttcagcttc
   106201 catcaacatt ggtgtgttat ggaaggctgg cgtgttctga ataaactcgc aaacttgcgt
   106261 atcgaccaga ataagtctgg cacgaccgcc tttcacgccg ggaaattttt ccgttttcca
   106321 gcctttttct ctgatccaac ggttaacagt ttgtttagcc acaccgaggc attcagccag
   106381 ttcttcggtg gtcattttgt tgcgtaatct tttcatatta tttgctatcg cgaatcccta
   106441 agcgttgtaa caatccgata atcccttccc gcagaagcaa ggatgtaaac tgtttttgtt
   106501 caactggcgt catctctttc gccagtgtca ccagcaatac ttcaagcggc gcatcaccag
   106561 aaagggcagg cgcttctccc tgaccttctg gacgttcagc attgcgaata tattcacgaa
   106621 cctgttcatt gacgtgaacc agtcgggctt tgccaccctg gacgccaggt tttggtgacg
   106681 ttgtccagcc ttccttgcgt acccatttat taatggtctg gcggctatag ccggtcagtc
   106741 tggcaagttc atctggtgtc atcctctcct tgaacataac aattccctga atgtatgtgg
   106801 gtctattagt agttcatttt ataacaccac tttacaggaa accgtgacat gaataactac
   106861 tgaatgcgag cggttttcca aaattgttca tttgcctgat ttttcagcga ttgaaattac
   106921 acgttgcaat ttgccgttga cacattcggg cggaatttat atgatgccgc ccgtcaactc
   106981 gacaagcttt acgtgacggg gctatagctc agttgggaga gcgcttgcat ggcatgcaag
   107041 aggtcagcgg ttcgatcccg cttagctcca ccaaattttg cacccagcaa atttggtacg
   107101 taaacgcatc gtggggctat agctcagctg ggagagcgct tgcatggcat gcaagaggtc
   107161 agcggttcga tcccgcttag ctccaccaaa tttccaaccc tcgctgcaaa gcgggggttt
   107221 tttgtctctg ctttttgccg cttttgtcat acagtctacg tccgggttag cgccgcctgg
   107281 tgaaagcatc attggatgaa aaatcggcaa caggctggcc cctgtttgct tcgcgatgcg
   107341 aataaactta ttatttgtgt gcctgaaaac cccgatcagt gagagtagtg tactcatgtt
   107401 tgtggagcat aacctgataa aaaatatcaa gatattcaca ctagcgttta cgctcaccgt
   107461 ggtacttatt cagctatccc gttttatttc gccacttgcc attatccatt ccagttatat
   107521 ctttctggcg tggatgccac tgtgcgtaat gctgtcaatc ttgtttatct ttggctggcg
   107581 cggtgtcgtt cccgttttat gcgggatgtt ttgcaccaat ctgtggaact ttcatctctc
   107641 ttttttacag actgcggtca tgctcggtag ccagacgttt gtcgtgttgt gtgcctgcgc
   107701 aatattacgc tggcagctgg ggacgcgttg gcgttatgga ttgaccagcc gatatgtctg
   107761 gcaacgtctg ttctggcttg gtttggtgac gccgatcggc atcaaatgca gcatgtatct
   107821 tgtggggagc ttctttgatt tcccgctaaa gatatccacc tttttcggcg atgcggatgc
   107881 cattttcacg gtcgttgatt tgctaagcct tttcaccgct gtgctgattt acaacatgct
   107941 tttctactat ctcactcgca tgattgtaag tccccacttt gcgcagatat tgtggcgcag
   108001 ggatatcgcg ccgtcgttgg gcaaagagaa acgcgcattt accttaagct ggctggcagc
   108061 gttaagcgtg cttctacttc tgttgtgcac accttatgaa aacgacttta ttgccggtta
   108121 cctggtaccc gttttcttca tcatctttac cctcggggtc ggtaagcttc gctatccgtt
   108181 tttaaatctc acctgggctg tttcaacgct ttgccttctg aattacaacc agaacttttt
   108241 gcaaggggtg gaaaccgaat attcgctggc atttattctt gcggtgctga tttcctttag
   108301 cgtttgcctg ctctatatgg tgcgcattta tcatcgcagt gaatggctta atcgccgctg
   108361 gcatttgcag gcgctgacag atccgttaac gctcctaccc aactttcgtg cgttggaaca
   108421 agcgccggaa caagaggcgg gcaagagttt ttgctgcctg cgcattgata atcttgagtt
   108481 tatgagtcgt cattacggct taatgatgcg cgttcactgt atccgctcaa tttgccgtac
   108541 gctgctgccg ttgatgcagg aaaacgaaaa gttgtatcaa ttgccgggta gtgaactgct
   108601 gttagtgctg agcgggccgg aaacggaagg gcgactccag catatgatta acatcctgaa
   108661 tagtcggcaa attcactgga acaataccgg gctggatatg ggctatggtg ctgcctgggg
   108721 gcgttttgat ggaaatcagg aaaccctgca acccttgttg gggcagttaa gctggctggc
   108781 ggagcaatcc tgcgcacatc atcatgtgct ggcgctggat agcagagagg agatggtttc
   108841 cgggcagacc actaaacagg tgctattgct gaataccatt cgcacggcgt tagatcaggg
   108901 tgatttgctg ctctacgccc agccaattcg caacaaagag ggtgaaggtt atgatgagat
   108961 cctcgcgcga ctgaaatatg acggcggcat tatgaccccg gataagtttc tgccccttat
   109021 tgctcagttt aaccttagcg cgcgttttga tttgcaagtg ctggaatcct tgttgaagtg
   109081 gctggcaaca cacccttgcg acaaaaaagg accgcgcttt tcagtcaatt taatgccgct
   109141 cacgctgctg caaaagaata ttgccgggcg gattattcgt ctgtttaagc gttatcacat
   109201 ctccccgcag gcggtcattc ttgagatcac cgaggagcag gcgttttcta acgcagaaag
   109261 cagcatgtac aacatcgagc agctgcataa gtttggtttc cggattgcga ttgatgactt
   109321 tggcaccgga tatgccaact acgaacggtt aaagcgtttg caggctgata tcatcaaaat
   109381 tgatggcgtc tttgtgaaag atattgtcac gaacacgctg gatgcgatga ttgtgagatc
   109441 aattaccgat ctggcgaaag cgaagtcatt gagtgtggtc gcggagtttg tcgagacgca
   109501 acagcagcag gcgctattgc ataagctcgg ggtgcaatat ctgcaagggt atttgattgg
   109561 tcgcccgcag ccattagctg attaacaggc gtaaaaaaac cggggaatta tccccggctt
   109621 ttttatgtat ggtcttacag caccagtgct gcgattgacg cagacagcac actcaccagg
   109681 gtagagccgt aaaccagctt cagaccgaag cgagaaacca cgttaccttg ctcttcattc
   109741 aggcctttaa ctgcacctgc gataatcccg attgaagaga agttagcgaa ggaaaccagg
   109801 aacacagaga tgatgccttc agcacgcgga gagagcgtgg aagcaatttt ctgcagatcc
   109861 atcatcgcaa cgaactcgtt ggaaaccagt ttggtcgcca tgatactgcc cacttgcagt
   109921 gcttcactgg aaggaacacc catcacccat gcaatcggat agaagatgta gcccaggatg
   109981 ccctggaagg agatgctgta gccaaaccag ccagtaacgg tggcaaacag tgcgttcagc
   110041 gcggcgatca gggcgataaa gccgatcagc atcgcggcaa cgataatggc aactttgaaa
   110101 cctgccagaa tgtattcacc cagcatttcg aagaagctct gaccttcgtg caggttggac
   110161 atctggatgt tttcttcact ggcatcaaca cggtaaggat tgatcagcga cagcacgata
   110221 aaggtgctga acatgttcag taccagcgca gcaacgacgt atttcggttc cagcatggtc
   110281 atgtatgcac caacgatgga catcgacacg gtggacattg ccgtggcagc catggtgtac
   110341 atacgattac gggagatttt gccgaggata tctttatagg caataaagtt ttcagactga
   110401 cccagaatca gggagctgac ggcgttaaag gattccagtt tgcccatacc gttgactttg
   110461 gagagcagga aaccaattgc gcggataata accggcaaca cgcgaatgtg ctggagaata
   110521 ccgatcagtg cagagataaa gacgattggg cacagcactt tcaggaagaa gaatgccagg
   110581 ccttgatcat tcatgctacc aaagacgaag ttagtccctt cgttggcaaa tccgagcagt
   110641 ttttcgaaca tttcggagaa gcctttcacg aagcctaaac caacgtcgga gttcaggaag
   110701 aaccacgcca gtaacacttc gataacaagc agttgaataa cataacggat acgaattttt
   110761 ttgcggtcgc tgcttaccag cagtgcgaga atcgcaacaa cggcaagtgc cagtacaaaa
   110821 tgaaggacgc ggtccatatt tgctccaaat atgaggcagg ttaaatttcc gtgcacattc
   110881 tatgtaacat ggttaaggaa aacgagatct gacacacact ataatgacgt cctgtgacaa
   110941 gcttcaaaaa tagtgatctg tcatacattt ctgctatgtt gtgtatggaa gctgaaagtt
   111001 atgtaaatgt gctaacatta aacataacca taacattcaa atcatcaatc ttttcgtagt
   111061 cataatcctg gtctatcaaa gaaatcacca caacccattc aaatgaattt gataatcatt
   111121 ctcgtttggc atagcatgaa acatagcaaa ggctatgttt tagaggcaca agatgacgaa
   111181 ctatcgcgtt gagagtagca gcggacgggc ggcgcgcaag atgaggctcg cattaatggg
   111241 acctgcgttc attgcggcga ttggttatat cgatcccggt aactttgcga ccaatattca
   111301 ggcgggtgct agcttcggct atcagctact gtgggttgtc gtttgggcca acctgatggc
   111361 gatgctgatt cagatcctct ctgccaaact agggattgcc accggtaaaa atctggcgga
   111421 gcagattcgt gatcactatc cgcgtcccgt agtgtggttc tattgggttc aggcagaaat
   111481 tattgcgatg gcaaccgacc tggcggaatt tattggtgcg gcgatcggtt ttaaactcat
   111541 tcttggtgtt tcgttgttgc agggcgcggt gctgacgggg atcgcgactt tcctgatttt
   111601 aatgctgcaa cgtcgcgggc aaaaaccgct ggagaaagtg attggcgggt tactgttgtt
   111661 tgttgccgcg gcttacattg tcgagttgat tttctcccag cctaacctgg cgcagttggg
   111721 taaaggaatg gtgatcccga gtttacctac ttcggaggcg gtcttcctgg cagcaggcgt
   111781 gttaggggcg acgattatgc cgcatgtgat ttatttgcac tcttcgctca ctcagcattt
   111841 acatggcggt tcgcgtcaac aacgttattc cgccaccaaa tgggatgtgg ctatcgccat
   111901 gacgattgcc ggttttgtca atctggcgat gatggctaca gctgcggcgg cgttccactt
   111961 ttctggtcat actggtgttg ccgatcttga tgaggcttat ctgacgctgc aaccgctgtt
   112021 aagccatgct gcggcaacgg tctttggatt aagtctggtt gctgccggac tgtcctcaac
   112081 ggtggtgggg acactggcgg ggcaggtggt gatgcaggga ttcattcgct tccatatccc
   112141 gctgtgggtg cgtcgtacag tcaccatgtt gccgtcattt attgtcattc tgatgggatt
   112201 agatccgaca cggattctgg ttatgagtca ggtgctgtta agttttggta tcgccctggc
   112261 gctggttcca ctgctgattt tcaccagtga cagcaagttg atgggcgatc tggtgaacag
   112321 caaacgcgta aaacagacag gctgggtgat tgtagtgctg gtcgtggcgc tgaatatctg
   112381 gttgttggtg gggacggcgc tgggattgta gttgaatgag cgtcgcatct ggcactattg
   112441 gcggatgcgg cgtaaacgcc ttatccgccc tacgcgttaa agagccggt
//
LOCUS       DAZFYC010000003       102794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFYC010000003 DAZFYC010000000
VERSION     DAZFYC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 102794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.003"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            427..1404
                     /gene="glaH"
                     /locus_tag="ACHKW5_000223"
     CDS             427..1404
                     /gene="glaH"
                     /locus_tag="ACHKW5_000223"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO7509677.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTFIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNIDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1424..2692
                     /gene="lhgO"
                     /locus_tag="ACHKW5_000224"
     CDS             1424..2692
                     /gene="lhgO"
                     /locus_tag="ACHKW5_000224"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO7509678.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRFDNCGKMLVATSPLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAIAKIFQD
                     RGGEIIYNAEVSALNEHKNGVVIRTSQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2715..4163
                     /gene="gabD"
                     /locus_tag="ACHKW5_000225"
     CDS             2715..4163
                     /gene="gabD"
                     /locus_tag="ACHKW5_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7509679.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4177..5457
                     /gene="gabT"
                     /locus_tag="ACHKW5_000226"
     CDS             4177..5457
                     /gene="gabT"
                     /locus_tag="ACHKW5_000226"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO7509680.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5695..7095
                     /gene="gabP"
                     /locus_tag="ACHKW5_000227"
     CDS             5695..7095
                     /gene="gabP"
                     /locus_tag="ACHKW5_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO7509681.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7116..7778
                     /gene="csiR"
                     /locus_tag="ACHKW5_000228"
     CDS             7116..7778
                     /gene="csiR"
                     /locus_tag="ACHKW5_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO7509682.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7779..8228)
                     /gene="kbp"
                     /locus_tag="ACHKW5_000229"
     CDS             complement(7779..8228)
                     /gene="kbp"
                     /locus_tag="ACHKW5_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO7509683.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8312..8470)
                     /gene="yqaE"
                     /locus_tag="ACHKW5_000230"
     CDS             complement(8312..8470)
                     /gene="yqaE"
                     /locus_tag="ACHKW5_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO7509684.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8653..8952
                     /gene="ygaV"
                     /locus_tag="ACHKW5_000231"
     CDS             8653..8952
                     /gene="ygaV"
                     /locus_tag="ACHKW5_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7509685.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8962..9486
                     /gene="ygaP"
                     /locus_tag="ACHKW5_000232"
     CDS             8962..9486
                     /gene="ygaP"
                     /locus_tag="ACHKW5_000232"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO7509686.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9533..9937)
                     /gene="stpA"
                     /locus_tag="ACHKW5_000233"
     CDS             complement(9533..9937)
                     /gene="stpA"
                     /locus_tag="ACHKW5_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO7509687.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10605..11054
                     /gene="alaE"
                     /locus_tag="ACHKW5_000234"
     CDS             10605..11054
                     /gene="alaE"
                     /locus_tag="ACHKW5_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO7509688.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(11091..11435)
                     /gene="ygaC"
                     /locus_tag="ACHKW5_000235"
     CDS             complement(11091..11435)
                     /gene="ygaC"
                     /locus_tag="ACHKW5_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO7509689.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            11587..11916
                     /gene="ygaM"
                     /locus_tag="ACHKW5_000236"
     CDS             11587..11916
                     /gene="ygaM"
                     /locus_tag="ACHKW5_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO7509690.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            12164..12409
                     /gene="nrdH"
                     /locus_tag="ACHKW5_000237"
     CDS             12164..12409
                     /gene="nrdH"
                     /locus_tag="ACHKW5_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO7509691.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            12406..12816
                     /gene="nrdI"
                     /locus_tag="ACHKW5_000238"
     CDS             12406..12816
                     /gene="nrdI"
                     /locus_tag="ACHKW5_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO7509692.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            12789..14933
                     /gene="nrdE"
                     /locus_tag="ACHKW5_000239"
     CDS             12789..14933
                     /gene="nrdE"
                     /locus_tag="ACHKW5_000239"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7509693.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            14943..15902
                     /gene="nrdF"
                     /locus_tag="ACHKW5_000240"
     CDS             14943..15902
                     /gene="nrdF"
                     /locus_tag="ACHKW5_000240"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7509694.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            16257..17459
                     /gene="proV"
                     /locus_tag="ACHKW5_000241"
     CDS             16257..17459
                     /gene="proV"
                     /locus_tag="ACHKW5_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO7509695.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            17452..18516
                     /gene="proW"
                     /locus_tag="ACHKW5_000242"
     CDS             17452..18516
                     /gene="proW"
                     /locus_tag="ACHKW5_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO7509696.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            18574..19566
                     /gene="proX"
                     /locus_tag="ACHKW5_000243"
     CDS             18574..19566
                     /gene="proX"
                     /locus_tag="ACHKW5_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO7509697.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            19858..21042
                     /locus_tag="ACHKW5_000244"
     CDS             19858..21042
                     /locus_tag="ACHKW5_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7509698.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            21166..21903
                     /gene="ygaZ"
                     /locus_tag="ACHKW5_000245"
     CDS             21166..21903
                     /gene="ygaZ"
                     /locus_tag="ACHKW5_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO7509699.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            21893..22228
                     /gene="ygaH"
                     /locus_tag="ACHKW5_000246"
     CDS             21893..22228
                     /gene="ygaH"
                     /locus_tag="ACHKW5_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO7509700.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLG
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            22319..22849
                     /gene="emrR"
                     /locus_tag="ACHKW5_000247"
     CDS             22319..22849
                     /gene="emrR"
                     /locus_tag="ACHKW5_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO7509701.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHLLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            22976..24148
                     /gene="emrA"
                     /locus_tag="ACHKW5_000248"
     CDS             22976..24148
                     /gene="emrA"
                     /locus_tag="ACHKW5_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO7509702.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            24165..25703
                     /gene="emrB"
                     /locus_tag="ACHKW5_000249"
     CDS             24165..25703
                     /gene="emrB"
                     /locus_tag="ACHKW5_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO7509703.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(25767..26282)
                     /gene="luxS"
                     /locus_tag="ACHKW5_000250"
     CDS             complement(25767..26282)
                     /gene="luxS"
                     /locus_tag="ACHKW5_000250"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO7509704.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(26432..27988)
                     /gene="gshA"
                     /locus_tag="ACHKW5_000251"
     CDS             complement(26432..27988)
                     /gene="gshA"
                     /locus_tag="ACHKW5_000251"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO7509705.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(28061..28489)
                     /gene="yqaA"
                     /locus_tag="ACHKW5_000252"
     CDS             complement(28061..28489)
                     /gene="yqaA"
                     /locus_tag="ACHKW5_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO7509706.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(28486..29052)
                     /gene="yqaB"
                     /locus_tag="ACHKW5_000253"
     CDS             complement(28486..29052)
                     /gene="yqaB"
                     /locus_tag="ACHKW5_000253"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO7509707.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(29333..29409)
                     /locus_tag="ACHKW5_000254"
     tRNA            complement(29333..29409)
                     /locus_tag="ACHKW5_000254"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29373..29375),aa:Arg,seq:acg)
     gene            complement(29608..29684)
                     /locus_tag="ACHKW5_000255"
     tRNA            complement(29608..29684)
                     /locus_tag="ACHKW5_000255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29648..29650),aa:Arg,seq:acg)
     gene            complement(29748..29824)
                     /locus_tag="ACHKW5_000256"
     tRNA            complement(29748..29824)
                     /locus_tag="ACHKW5_000256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29788..29790),aa:Arg,seq:acg)
     gene            complement(30022..30098)
                     /locus_tag="ACHKW5_000257"
     tRNA            complement(30022..30098)
                     /locus_tag="ACHKW5_000257"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30062..30064),aa:Arg,seq:acg)
     gene            complement(30102..30194)
                     /locus_tag="ACHKW5_000258"
     tRNA            complement(30102..30194)
                     /locus_tag="ACHKW5_000258"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30158..30160),aa:Ser,seq:gct)
     gene            complement(30510..30695)
                     /gene="csrA"
                     /locus_tag="ACHKW5_000259"
     CDS             complement(30510..30695)
                     /gene="csrA"
                     /locus_tag="ACHKW5_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO7509708.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(30930..33560)
                     /gene="alaS"
                     /locus_tag="ACHKW5_000260"
     CDS             complement(30930..33560)
                     /gene="alaS"
                     /locus_tag="ACHKW5_000260"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO7509709.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAEGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(33688..34188)
                     /gene="recX"
                     /locus_tag="ACHKW5_000261"
     CDS             complement(33688..34188)
                     /gene="recX"
                     /locus_tag="ACHKW5_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO7509710.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(34431..35492)
                     /gene="recA"
                     /locus_tag="ACHKW5_000262"
     CDS             complement(34431..35492)
                     /gene="recA"
                     /locus_tag="ACHKW5_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO7509711.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(35572..36069)
                     /gene="pncC"
                     /locus_tag="ACHKW5_000263"
     CDS             complement(35572..36069)
                     /gene="pncC"
                     /locus_tag="ACHKW5_000263"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO7509712.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(36214..37299)
                     /gene="mltB"
                     /locus_tag="ACHKW5_000264"
     CDS             complement(36214..37299)
                     /gene="mltB"
                     /locus_tag="ACHKW5_000264"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO7509713.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37555..38118
                     /gene="srlA"
                     /locus_tag="ACHKW5_000265"
                     /gene_synonym="gutA"
     CDS             37555..38118
                     /gene="srlA"
                     /locus_tag="ACHKW5_000265"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO7509714.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPISRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            38115..39074
                     /gene="srlE"
                     /locus_tag="ACHKW5_000266"
                     /gene_synonym="gutE"
     CDS             38115..39074
                     /gene="srlE"
                     /locus_tag="ACHKW5_000266"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO7509715.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            39085..39456
                     /gene="srlB"
                     /locus_tag="ACHKW5_000267"
                     /gene_synonym="gutB"
     CDS             39085..39456
                     /gene="srlB"
                     /locus_tag="ACHKW5_000267"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO7509716.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            39460..40239
                     /gene="srlD"
                     /locus_tag="ACHKW5_000268"
     CDS             39460..40239
                     /gene="srlD"
                     /locus_tag="ACHKW5_000268"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO7509717.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            40344..40703
                     /gene="gutM"
                     /locus_tag="ACHKW5_000269"
     CDS             40344..40703
                     /gene="gutM"
                     /locus_tag="ACHKW5_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO7509718.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            40770..41543
                     /gene="srlR"
                     /locus_tag="ACHKW5_000270"
                     /gene_synonym="gutR"
     CDS             40770..41543
                     /gene="srlR"
                     /locus_tag="ACHKW5_000270"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO7509719.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            41536..42501
                     /gene="gutQ"
                     /locus_tag="ACHKW5_000271"
     CDS             41536..42501
                     /gene="gutQ"
                     /locus_tag="ACHKW5_000271"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO7509720.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALAASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(42498..44012)
                     /gene="norR"
                     /locus_tag="ACHKW5_000272"
     CDS             complement(42498..44012)
                     /gene="norR"
                     /locus_tag="ACHKW5_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO7509721.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLIYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            44199..45638
                     /gene="norV"
                     /locus_tag="ACHKW5_000273"
     CDS             44199..45638
                     /gene="norV"
                     /locus_tag="ACHKW5_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO7509722.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            45635..46768
                     /gene="norW"
                     /locus_tag="ACHKW5_000274"
     CDS             45635..46768
                     /gene="norW"
                     /locus_tag="ACHKW5_000274"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO7509723.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIASTGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(46994..49246)
                     /gene="hypF"
                     /locus_tag="ACHKW5_000275"
     CDS             complement(46994..49246)
                     /gene="hypF"
                     /locus_tag="ACHKW5_000275"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO7509724.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(49399..49926)
                     /gene="hydN"
                     /locus_tag="ACHKW5_000276"
     CDS             complement(49399..49926)
                     /gene="hydN"
                     /locus_tag="ACHKW5_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO7509725.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(50075..51085)
                     /gene="ascG"
                     /locus_tag="ACHKW5_000277"
     CDS             complement(50075..51085)
                     /gene="ascG"
                     /locus_tag="ACHKW5_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO7509726.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            51345..52802
                     /gene="ascF"
                     /locus_tag="ACHKW5_000278"
     CDS             51345..52802
                     /gene="ascF"
                     /locus_tag="ACHKW5_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO7509727.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            52811..54235
                     /gene="ascB"
                     /locus_tag="ACHKW5_000279"
     CDS             52811..54235
                     /gene="ascB"
                     /locus_tag="ACHKW5_000279"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO7509728.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     KITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(54349..54768)
                     /locus_tag="ACHKW5_000280"
     CDS             complement(54349..54768)
                     /locus_tag="ACHKW5_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7509729.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(54839..55593)
                     /locus_tag="ACHKW5_000281"
                     /pseudo
     CDS             complement(54839..55593)
                     /locus_tag="ACHKW5_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(55636..56106)
                     /gene="hycI"
                     /locus_tag="ACHKW5_000282"
     CDS             complement(55636..56106)
                     /gene="hycI"
                     /locus_tag="ACHKW5_000282"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO7509730.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(56099..56509)
                     /gene="hycH"
                     /locus_tag="ACHKW5_000283"
     CDS             complement(56099..56509)
                     /gene="hycH"
                     /locus_tag="ACHKW5_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO7509731.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(56506..57273)
                     /gene="hycG"
                     /locus_tag="ACHKW5_000284"
     CDS             complement(56506..57273)
                     /gene="hycG"
                     /locus_tag="ACHKW5_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO7509732.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(57273..57815)
                     /gene="hycF"
                     /locus_tag="ACHKW5_000285"
     CDS             complement(57273..57815)
                     /gene="hycF"
                     /locus_tag="ACHKW5_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO7509733.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(57825..59534)
                     /gene="hycE"
                     /locus_tag="ACHKW5_000286"
     CDS             complement(57825..59534)
                     /gene="hycE"
                     /locus_tag="ACHKW5_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO7509734.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(59552..60475)
                     /gene="hycD"
                     /locus_tag="ACHKW5_000287"
     CDS             complement(59552..60475)
                     /gene="hycD"
                     /locus_tag="ACHKW5_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO7509735.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(60478..62304)
                     /gene="hycC"
                     /locus_tag="ACHKW5_000288"
     CDS             complement(60478..62304)
                     /gene="hycC"
                     /locus_tag="ACHKW5_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO7509736.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(62301..62912)
                     /gene="hycB"
                     /locus_tag="ACHKW5_000289"
     CDS             complement(62301..62912)
                     /gene="hycB"
                     /locus_tag="ACHKW5_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO7509737.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(63037..63498)
                     /gene="hycA"
                     /locus_tag="ACHKW5_000290"
     CDS             complement(63037..63498)
                     /gene="hycA"
                     /locus_tag="ACHKW5_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO7509738.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            63710..64060
                     /gene="hypA"
                     /locus_tag="ACHKW5_000291"
     CDS             63710..64060
                     /gene="hypA"
                     /locus_tag="ACHKW5_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7509739.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            64064..64936
                     /gene="hypB"
                     /locus_tag="ACHKW5_000292"
     CDS             64064..64936
                     /gene="hypB"
                     /locus_tag="ACHKW5_000292"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO7509740.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            64927..65199
                     /gene="hypC"
                     /locus_tag="ACHKW5_000293"
     CDS             64927..65199
                     /gene="hypC"
                     /locus_tag="ACHKW5_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO7509741.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            65199..66320
                     /gene="hypD"
                     /locus_tag="ACHKW5_000294"
     CDS             65199..66320
                     /gene="hypD"
                     /locus_tag="ACHKW5_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO7509742.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            66317..67327
                     /gene="hypE"
                     /locus_tag="ACHKW5_000295"
     CDS             66317..67327
                     /gene="hypE"
                     /locus_tag="ACHKW5_000295"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO7509743.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            67401..69479
                     /gene="flhA"
                     /locus_tag="ACHKW5_000296"
     CDS             67401..69479
                     /gene="flhA"
                     /locus_tag="ACHKW5_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO7509744.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(69516..69869)
                     /gene="ygbA"
                     /locus_tag="ACHKW5_000297"
     CDS             complement(69516..69869)
                     /gene="ygbA"
                     /locus_tag="ACHKW5_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO7509745.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(69901..70080)
                     /locus_tag="ACHKW5_000298"
     CDS             complement(69901..70080)
                     /locus_tag="ACHKW5_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509746.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            70156..72717
                     /gene="mutS"
                     /locus_tag="ACHKW5_000299"
     CDS             70156..72717
                     /gene="mutS"
                     /locus_tag="ACHKW5_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO7509747.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            72823..73479
                     /gene="pphB"
                     /locus_tag="ACHKW5_000300"
     CDS             72823..73479
                     /gene="pphB"
                     /locus_tag="ACHKW5_000300"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7509748.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(73530..74297)
                     /gene="ygbI"
                     /locus_tag="ACHKW5_000301"
     CDS             complement(73530..74297)
                     /gene="ygbI"
                     /locus_tag="ACHKW5_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO7509749.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            74493..75401
                     /gene="ltnD"
                     /locus_tag="ACHKW5_000302"
     CDS             74493..75401
                     /gene="ltnD"
                     /locus_tag="ACHKW5_000302"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO7509750.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            75398..76660
                     /gene="otnK"
                     /locus_tag="ACHKW5_000303"
     CDS             75398..76660
                     /gene="otnK"
                     /locus_tag="ACHKW5_000303"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO7509751.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWTQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            76657..77295
                     /gene="ygbL"
                     /locus_tag="ACHKW5_000304"
     CDS             76657..77295
                     /gene="ygbL"
                     /locus_tag="ACHKW5_000304"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO7509752.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            77300..78076
                     /gene="ygbM"
                     /locus_tag="ACHKW5_000305"
     CDS             77300..78076
                     /gene="ygbM"
                     /locus_tag="ACHKW5_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO7509753.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            78165..79529
                     /gene="ygbN"
                     /locus_tag="ACHKW5_000306"
     CDS             78165..79529
                     /gene="ygbN"
                     /locus_tag="ACHKW5_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO7509754.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(79568..79822)
                     /locus_tag="ACHKW5_000307"
     CDS             complement(79568..79822)
                     /locus_tag="ACHKW5_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO7509755.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRDT
                     EPLRRTSREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(79833..81260)
                     /locus_tag="ACHKW5_000308"
     CDS             complement(79833..81260)
                     /locus_tag="ACHKW5_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO7509756.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(81260..81853)
                     /locus_tag="ACHKW5_000309"
     CDS             complement(81260..81853)
                     /locus_tag="ACHKW5_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO7509757.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            82000..82407
                     /locus_tag="ACHKW5_000310"
     CDS             82000..82407
                     /locus_tag="ACHKW5_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO7509758.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(82528..83520)
                     /gene="rpoS"
                     /locus_tag="ACHKW5_000311"
     CDS             complement(82528..83520)
                     /gene="rpoS"
                     /locus_tag="ACHKW5_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO7509759.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(83583..84677)
                     /gene="nlpD"
                     /locus_tag="ACHKW5_000312"
     CDS             complement(83583..84677)
                     /gene="nlpD"
                     /locus_tag="ACHKW5_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO7509760.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKMIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(84862..85488)
                     /gene="pcm"
                     /locus_tag="ACHKW5_000313"
     CDS             complement(84862..85488)
                     /gene="pcm"
                     /locus_tag="ACHKW5_000313"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO7509761.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(85482..86243)
                     /gene="surE"
                     /locus_tag="ACHKW5_000314"
     CDS             complement(85482..86243)
                     /gene="surE"
                     /locus_tag="ACHKW5_000314"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO7509762.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(86224..87273)
                     /gene="truD"
                     /locus_tag="ACHKW5_000315"
     CDS             complement(86224..87273)
                     /gene="truD"
                     /locus_tag="ACHKW5_000315"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO7509763.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(87270..87749)
                     /gene="ispF"
                     /locus_tag="ACHKW5_000316"
     CDS             complement(87270..87749)
                     /gene="ispF"
                     /locus_tag="ACHKW5_000316"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO7509764.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(87749..88459)
                     /gene="ispD"
                     /locus_tag="ACHKW5_000317"
     CDS             complement(87749..88459)
                     /gene="ispD"
                     /locus_tag="ACHKW5_000317"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO7509765.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(88478..88789)
                     /gene="ftsB"
                     /locus_tag="ACHKW5_000318"
     CDS             complement(88478..88789)
                     /gene="ftsB"
                     /locus_tag="ACHKW5_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO7509766.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(88983..89306)
                     /gene="ygbE"
                     /locus_tag="ACHKW5_000319"
     CDS             complement(88983..89306)
                     /gene="ygbE"
                     /locus_tag="ACHKW5_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO7509767.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(89356..89961)
                     /gene="cysC"
                     /locus_tag="ACHKW5_000320"
     CDS             complement(89356..89961)
                     /gene="cysC"
                     /locus_tag="ACHKW5_000320"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO7509768.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGVLEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(89961..91388)
                     /gene="cysN"
                     /locus_tag="ACHKW5_000321"
     CDS             complement(89961..91388)
                     /gene="cysN"
                     /locus_tag="ACHKW5_000321"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO7509769.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(91390..92298)
                     /gene="cysD"
                     /locus_tag="ACHKW5_000322"
     CDS             complement(91390..92298)
                     /gene="cysD"
                     /locus_tag="ACHKW5_000322"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO7509770.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            92550..93587
                     /gene="iap"
                     /locus_tag="ACHKW5_000323"
     CDS             92550..93587
                     /gene="iap"
                     /locus_tag="ACHKW5_000323"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO7509771.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   93669..94125
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=93730..93758
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(94222..94515)
                     /gene="cas2e"
                     /locus_tag="ACHKW5_000324"
     CDS             complement(94222..94515)
                     /gene="cas2e"
                     /locus_tag="ACHKW5_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO7509772.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(94515..95435)
                     /gene="cas1e"
                     /locus_tag="ACHKW5_000325"
     CDS             complement(94515..95435)
                     /gene="cas1e"
                     /locus_tag="ACHKW5_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO7509773.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(95432..96082)
                     /gene="cas6e"
                     /locus_tag="ACHKW5_000326"
     CDS             complement(95432..96082)
                     /gene="cas6e"
                     /locus_tag="ACHKW5_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO7509774.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(96064..96810)
                     /gene="cas5e"
                     /locus_tag="ACHKW5_000327"
     CDS             complement(96064..96810)
                     /gene="cas5e"
                     /locus_tag="ACHKW5_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO7509775.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(96821..97894)
                     /gene="cas7e"
                     /locus_tag="ACHKW5_000328"
     CDS             complement(96821..97894)
                     /gene="cas7e"
                     /locus_tag="ACHKW5_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO7509776.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPGFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            complement(97909..98442)
                     /gene="casB"
                     /locus_tag="ACHKW5_000329"
                     /gene_synonym="cse2"
     CDS             complement(97909..98442)
                     /gene="casB"
                     /locus_tag="ACHKW5_000329"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO7509777.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            complement(98439..100001)
                     /gene="casA"
                     /locus_tag="ACHKW5_000330"
                     /gene_synonym="cse1"
     CDS             complement(98439..100001)
                     /gene="casA"
                     /locus_tag="ACHKW5_000330"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO7509778.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRAFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(100099..>102794)
                     /gene="cas3"
                     /locus_tag="ACHKW5_000331"
     CDS             complement(100099..>102794)
                     /gene="cas3"
                     /locus_tag="ACHKW5_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO7509779.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVA
                     AMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPEL
                     KIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGH
                     HGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSWYI
                     SATVVLADWTGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGINT
                     LFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGL
                     FFGLPTMATANAMYDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLIGS
                     EEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQQE
                     APLLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQGK
                     CIAWIRNSVDDAIQVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCS
                     LQRASQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRDGK
                     DERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKYVLNFRPGYAANINDYLP
                     EKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     DAFRQWCVEQRQDPEMANVILVTDDESCGYSAREGLIGKVG"
BASE COUNT        24234 a        27621 c        26402 g        24537 t
ORIGIN      
        1 tagaggcgga actctaaata tcaatattcc tgggcgtagc gctcttcttt tagggaaaac
       61 aggagaaccg ctgaactatc tctatttgta atttaatgtt atatctgccc cgctaaaacg
      121 gggcagataa tatgtttagt ttactaacgg tcattttgca ttgaagccat ttactgtttt
      181 ttatcgacca gataatctgt tctctgatgg taactccccc taacctattg ctttagttat
      241 tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt
      301 tgatcacaat aagaaaacaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt
      361 tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg
      421 cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg ccaggactat
      481 agcggattca ctttcatccc gtcggcgcaa tccccgcgtc tgctggaact taccttcacc
      541 gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtaca ggcgctggag
      601 tacaaatcct ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag
      661 ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat
      721 gcggtgggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta
      781 gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc
      841 gtggtgaaaa atatcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa
      901 ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc
      961 gatgagcaaa acatgcaggg tggaaattcg ttgctgctgc atctcgatga ctgggaacat
     1021 ctggaccact atttccgcca tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg
     1081 agcaaaaacg tcagcaaaga tgttttccat ccagtgttcg acgttgatca acagggtcgc
     1141 ccggtgatgc gctatatcga ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg
     1201 ttgagcgagc tttccgatgc cattgaaacc agcaaaggca ttctttctgt acccgttccc
     1261 gttggcaaat tcctgttgat aaacaaccta ttctggctgc acggtcgcga ccgctttact
     1321 ccgcacccgg atctgcgccg tgaactgatg cgtcagcgtg gctatttcgc ttacgcctct
     1381 aaccactacc agacgcatca gtaagcgcaa aggaattgag cggatgtatg attttgtgat
     1441 tattggcggc ggcatcatcg gcatgtcgac cgccatgcaa ctgattgatg tctatccgga
     1501 tgcccgcatt gcgttgctgg aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa
     1561 cagcggcgtg atccatgccg gggtctatta cacgcccggt agcctgaagg cacagttttg
     1621 cctggcggga aaccgcgcca ctaaagcctt ttgcgatcaa aacggcattc gctttgacaa
     1681 ctgcggcaag atgctggtcg ccacgtctcc gctcgaaatg gaacggatgc gcgccttatg
     1741 ggaacgcaca gcggcgaacg gtatcgagcg cgaatggcta aacgccgacg aactgcgcga
     1801 gcgcgaaccg aatatcaccg ggctcggcgg tatttttgtg ccgtccagcg gcattgtcag
     1861 ctaccgcgaa gtgacagcgg cgatagcaaa aatctttcag gacagaggcg gcgagattat
     1921 ttataacgcc gaagtcagcg cccttaatga gcataaaaac ggcgtggtga tacgtaccag
     1981 tcagggcggt gaatatgaag catcgacgct gattagctgt tccgggctga tggctgaccg
     2041 gctggtgaaa atgctcggac tcgaaccggg ctttatcatc tgcccgttcc gtggcgagta
     2101 tttccgcctt gcgccggagc ataaccagat tgttaaccac ctgatttacc ccatccccga
     2161 cccggcaatg ccgtttttgg gcgtgcatct cacccgcatg atcgacggca gcgtgaccgt
     2221 cgggccaaac gcggtgctgg ctttcaaacg tgaaggctat cgcaagcgcg acttctcatt
     2281 tagcgacacg ctggaaattt tgggctcgtc ggggattcgc cgggtgctgc aaaaccatct
     2341 acgctcagga ctgggcgaga tgaaaaactc gctgtgcaaa agcggctatc tgcggctggt
     2401 gcaaaagtat tgtccccggc tttcgttaag cgatctccag ccctggcccg ccggtgtgcg
     2461 ggcgcaggcg gtatcgccgg acggcaagct gattgacgat tttctgtttg tcaccacccc
     2521 gcgcacgatc cacacctgca atgcgccctc cccggcagcg acatcagcaa ttcctattgg
     2581 tgcgcatatt gtcagcaagg tacaaacgct gttggcaagc cagagtaacc ccggacgcac
     2641 gctgcgagcg gcacgtagtg tggatgcctt acacgccgca tttaatcaat aacctttgaa
     2701 aacaggacgt aacaatgaaa cttaacgaca gtaacttatt ccgccagcag gcgttgatta
     2761 acggggaatg gctggacgcc aacaatggcg aggtcatcga cgtcaccaat ccggcgaacg
     2821 gcgacaagct gggtagcgtg ccgaaaatgg gcgcggatga aacccgcgcc gctatcgacg
     2881 ccgccaaccg cgccctgcca gcctggcgcg cgctcaccgc caaagaacgc gccaccattc
     2941 tgcgcaactg gttcaatttg atgatggagc atcaggacga tttagcgcgc ctgatgaccc
     3001 tcgaacaggg taaaccgctg gctgaagcga aaggcgaaat cagctacgcc gcttccttta
     3061 ttgagtggtt tgccgaagaa ggcaaacgca tttatggcga caccattccc ggtcatcagg
     3121 ccgataagcg cctgattgtt atcaagcagc cgattggcgt taccgccgcc atcacgccgt
     3181 ggaacttccc ggcggcgatg attacccgta aagccggtcc ggcgctggcg gcaggctgca
     3241 cgatggtgct gaaacccgcc agtcagacgc cgttctctgc gctggcgctg gcggagctgg
     3301 cgattcgcgc gggcattccg gctggcgtat ttaacgtggt caccggttcg gcgggcgcgg
     3361 tgggtaacga actgaccagc aacccgctgg tgcgcaaact gtcgtttacc ggttcgaccg
     3421 aaattggccg ccagttaatg gaacagtgcg cgaaagacat caagaaagtg tcgctggagc
     3481 tgggcggcaa cgcgccgttt atcgtctttg atgatgccga tctcgacaaa gccgtggaag
     3541 gcgcactggc ctcgaaattc cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat
     3601 acgtgcagga cggcgtgtat gaccgctttg ccgaaaaatt acagcaggcg gtgagcaaac
     3661 tgcacatcgg cgacgggctg gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag
     3721 cggtagcaaa agtggaagag catattgccg atgcgctgga gaaaggtgcg cgtgtggttt
     3781 gcggcggtaa agcggatgaa cgcggcggca acttcttcca gccgaccatt ctggtggacg
     3841 ttccagccaa cgccaaagtg tcgaaagaag agacgttcgg ccccctcgcc ccactgttcc
     3901 gttttaaaga tgaagccgat gtgattgcgc aagccaatga caccgagttt ggccttgccg
     3961 cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg
     4021 gcatcgtcgg catcaatacc ggcatcattt ccaatgaagt ggccccgttc ggcggcatta
     4081 aagcctcggg tctgggtcgt gaaggttcga agtatggcat cgaagattac ttagaaatca
     4141 aatatatgtg catcggtctt taactggaga acgcgaatga gcagcaataa agagttaatg
     4201 cagcgccgca gtcaggcgat tccccgtggt gttgggcaaa ttcacccgat tttcgctgac
     4261 cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc
     4321 gggattgcgg tgctcaatac cgggcacttg catccgaaag tggtggctgc ggtggaagcg
     4381 cagttgaaaa aactgtcgca cacctgcttc caggtgctgg cctacgagcc gtatctggag
     4441 ctgtgcgaga ttatgaatca gaaggtgcca ggcaattttg ccaagaaaac gctgctggtc
     4501 accacaggtt ctgaagcggt ggaaaacgcg gtgaaaatcg cccgcgccgc caccaaacgt
     4561 agcggcacca tcgcttttag cggcgcgtat cacgggcgca cgcattacac gctggcgctg
     4621 accggcaagg tgaatccgta ctctgcgggc atggggctga tgccgggtca tgtttatcgc
     4681 gcgctttatc cttgcccgct gcacggcata agcgaggatg acgctatcgc cagcatccac
     4741 cggatcttca aaaatgatgc cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt
     4801 cagggcgaag gcggtttcta cgcctcgtcg ccagccttta tgcagcgttt acgcgctctg
     4861 tgtgacgagc acgggatcat gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc
     4921 ggcacgctgt ttgcgatgga gcagatgggc gttgcgccgg atcttaccac ctttgcgaaa
     4981 tcgatcgcgg gcggcttccc gctggcgggc gtcaccgggc gcgcggaagt aatggatgcc
     5041 gtcgctccag gcggtctggg cggcacctat gcgggtaacc cgattgcctg cgtggctgcg
     5101 ctggaagtgt tgaaggtgtt tgagcaggaa aatctgctgc aaaaagccaa cgatctgggg
     5161 cagaagttga aagacggatt gctggcaata gccgaaaaac acccggagat cggcgacgta
     5221 cgcgggctgg gggcgatgat cgccattgag ctgtttgaag acggcgatca caacaagccg
     5281 gacgccaaac tcaccgccga gatcgtggct cgcgcccgcg ataaaggcct gattcttctc
     5341 tcctgcggcc cgtattacaa cgtgctgcgc atccttgtac cgctcaccat tgaagacgct
     5401 cagatccgtc agggtctgga gatcatcagc cagtgttttg atgaggcgaa gcagtagcgc
     5461 cgctcctatg ccggaggcga cgctgcgcgt cttgtccggc ctacggggat ccaggtcgga
     5521 taaggcgttt acgccgcatc cgacaatctg tacgtgaaca ggaagaaatc tatgttggcc
     5581 gggtaaggcg gagccgctct ccggcaaaaa gaatcaataa caattatacg cgtgacccgg
     5641 cgcgggaaat gtcggggcgc tctcccaagt gacacacttt cgagaggatt caggatgggg
     5701 caatcatcgc aaccacatga gttaggcggc gggctgaagt cacgccacgt caccatgttg
     5761 tctattgccg gtgttatcgg cgcaagtctg tttgtcggtt ccagcgtcgc catcgccgaa
     5821 gcgggcccgg cggtattact ggcctatctg ttcgccgggc tactggtggt tatgattatg
     5881 cggatgttgg cggaaatggc ggttgccacg cccgataccg gttcgttttc cacctatgcc
     5941 gataaagcca ttggtcgctg ggcgggctat accatcggct ggttgtactg gtggttttgg
     6001 gtactggtta tcccgctgga agccaacatc gccgccatga tcctgcactc atgggttcca
     6061 ggcattccca tctggttatt ttccctcgtc attaccctcg ccttaactgg cagtaactta
     6121 ttaagcgtta aaaactacgg cgaatttgag ttctggctgg cgctgtgcaa agtcatcgct
     6181 atcctggcct ttattttcct tggtgcagtc gcaattagcg gtttttaccc gtatgccgaa
     6241 gtgagcggga tctcaagatt gtgggatagc ggcggcttta tgcccaacgg tttcggtgcg
     6301 gtattaagcg cgatgttgat caccatgttc tcgtttatgg gcgcagaaat tgtcaccatt
     6361 gccgccgcgg aatccgacac gccggaaaaa catattgtcc gcgccaccaa ctcggttatc
     6421 tggcgtattt ctatcttcta tttatgttct atttttgtcg tcgtggcatt aattccgtgg
     6481 aatatgcccg gactaaaagc cgtcggttct tatcgctcgg tactggaatt gctcaatatt
     6541 ccccatgcga aattaatcat ggactgcgtg atattacttt ccgtaaccag ttgcctgaac
     6601 tcggcgctgt ataccgcgtc aaggatgctc tactccttaa gccgtcgcgg tgatgctccc
     6661 gcggtaatgg gtaaaatcaa ccgcagtaaa accccgtacg tggcggtgtt actctccacc
     6721 ggcgcggcat tcttaacggt ggtggtgaac tattacgcgc ctgcgaaggt atttaaattt
     6781 ctgatcgaca gctccggcgc tatcgccctg ctggtttatt tagtcatcgc cgtttcacag
     6841 ttgcggatgc gcaaaattct gcgagcagaa ggaagcgaaa ttcgcttgcg aatgtggctt
     6901 tatccgtggc tcacctggct agtcatcggc tttattacct ttgtgttggt agtgatgcta
     6961 ttccgtccgg cgcaacagtt agaagtgatc tccaccggct tattagcgat agggattatc
     7021 tgtaccgtgc caattatggc tcgctggaaa aagctggtat tgtggcaaaa aacacccgtt
     7081 cataatacgc gctgatcatg atcaggagtc acaccatgac cattacgtct ctggatggct
     7141 atcgctggct gaagaacgat attattcgcg gtaattttca accggatgaa aaattacgaa
     7201 tgagtttgct gacatcgcgt tatgcacttg gcgttgggcc gttacgggaa gctctttcgc
     7261 aactggtggc ggaacggctg gtcacggtgg tgaatcaaaa agggtatcgg gtggcgtcta
     7321 tgtcagagca ggagttgctc gatattttcg acgcccgcgc caatatggaa gcgatgttag
     7381 tgagtctggc gattgcccgc ggtggcgatg agtgggaggc agacgttctc gcaaaagcgc
     7441 atctgctgag taagcttgag gcctgtgacg ccagcgagaa aatgcttgat gagtgggatc
     7501 tgcgtcatca ggcgtttcat acggcaattg tggcgggctg tggttctcac tatttgctgc
     7561 aaatgcgtga acggttgttt gatctggcgg cgcgctatcg atttatctgg ctgcggcgaa
     7621 cggtgctttc ggtagaaatg ctggaggata aacacgatca gcaccagacc ctgactgcgg
     7681 cggtactggc gcgagatacc gcgcgcgcca gtgagttaat gcgccagcat ttactgacgc
     7741 caattcccat tatccagcag gcgatggctg gcaattaact actcttccgg aatacgcaac
     7801 acttgccccg gataaatttt atccgggctt tttagcatcg gtttattcgc ttcgaagatt
     7861 ttattgtaca gattagcgtt accgtagacc tgtttggaaa tggcactcag agtgtcgcca
     7921 gacttaacgg tataaaactg gctggcagtg gctggtgtcg ccgttttcac ctgatcatca
     7981 acactggcaa taccggaaat attcccaacc gcaacaagga ttttctcctt cgcctcctga
     8041 ctcaggccgt caccagtgac cgtcgctttg ccgtcggcaa tttgaatgtt cactttatcg
     8101 gcatcaggta taccggtttt gctcagatgc tcctgcacct tcttcgcctg atcgtcttta
     8161 tcgtgctgac ctgtaaccgc gtcccagagt ttttctccgg catctttcac aaaattgaac
     8221 agacccatag ctacctcatt cgttaacgga aacactcaga aagtgtagca gaggaacgcg
     8281 gcgcgttaac gccgcggggg tgagcacagg tttaatcgcg ggtttgcacc cagaacgcgt
     8341 gaatcaaacc ggggatatac ccaagcagtg tcagcagaat attaataatg aacgcccaac
     8401 cgaacccttt accgagcagc acgccgagcg gcggcagaat gatggtgatg acgattctcc
     8461 agaaacccat atgtactccc tataagaaaa ttactcattg tttaaaaaga gattttatct
     8521 cttaagcgta gtaattttag cggagcctgc cagtttcagc ttacgctttt actctcattt
     8581 acgctgctga ggctggtata tgttgcattt ttagtttaga gtttacttaa tttagaaaat
     8641 acttaaataa atatgactga actcgcgcaa ttacaggcca gtgccgaaca ggcagcggcc
     8701 ttattgaaag caatgagcca tcctaaacga ttgctgattc tgtgcatgct tagcggttcc
     8761 cccggcacca gcgcgggaga gctgacgcgc attaccggac tgagtgcctc tgcgacatca
     8821 cagcatctcg ctcgtatgcg ggacgaaggg cttatcgaca gccaacggga tgcccaacgc
     8881 attctatatt ccattaaaaa tgaggcggta aatgccatta tcgccaccct gaaaaatgtc
     8941 tattgtccgt aaggagtcac tatggctttg acaaccattt cgccgcatga tgcacaagaa
     9001 ttaatcgcac gcggcgcaaa gttaatcgat attcgtgacg ctgatgaata tcttcgtgaa
     9061 catattcctg aagcagatct ggctccatta tccgtgctgg aacagtcagg tcttccacct
     9121 aaattacgtc gcgagcaaat tatattccac tgccaggcag gtaaacgcac cagtaataac
     9181 gccgataaat tagcagcgat tgccgccccc gcagaaatat ttttactcga agatgggatt
     9241 gatggctgga aaagagctgg attgccagta gcggtaaata aatctcaacc cttgccgtta
     9301 atgcgccagg tgcagatcgc tgcgggtggt ttaatattaa tcggcgttgt actgggttat
     9361 accgtaaata gcggtttctt cttattaagt ggctttgttg gtgccgggtt actgtttgca
     9421 ggaatcagcg gtttttgtgg aatggcaagg ttgttagata agatgccgtg gaaccaacga
     9481 acttgagaag cgacgccgga cgcgccctag cagcgacatc cggcctcagt aattagatca
     9541 ggaaatcgtc gagagattta ccttctgcca gcgcctgagc aattggcttc ggtgtacggc
     9601 cctgaccggt ccaggtttta gtttcaccgt taacatcggt gaatttatat ttcgccggac
     9661 gcggctggcg ttttttacca gcgcgtggtg cagcagcaga gctattacct aataactctt
     9721 ccgggttaat tccgtcagcc ttcatcagct ccagccaggt gctaattttt tcctgacgct
     9781 cagccagttc acgctgctgc tgttcttctt cttcacgtct ttctttagtg acaaccctga
     9841 atttttcgag catttcttca agaacgtcaa tggagaattc gcgagccatc gcacggagag
     9901 tgcgaatgtt atttaaactt tgtaacatta cggacataat aaagaaaacc ttttaacgcc
     9961 aaaacaaaaa agtatttcgt cgcgtagtgt aagcgatcac ttattatatt tccacaaaat
    10021 ttaatctatt tacagtcctg tgcgagatta tttcagctgt tattttctga aactctcaga
    10081 ataatttcaa gattatatat tagccatttt gatgagtaat ttagattatt ggtgaacaga
    10141 acggcgctct ctgctcgcta attccgcaag cctgacaaaa ccgaagtgaa taaaaatgca
    10201 atataacaga ataaaagaaa acacccctca ctggtgcttt ctgctgttaa tgtacatttt
    10261 ctggcataaa aaatactgta aatgacacgg tcacgacata caccgaattc tctacgctta
    10321 tagcgttacc tcacccccaa actttcatta gtgaaattaa aggaaaggtc atatccaatt
    10381 gatttaattg aaaataaatc atatcggcat gtaaaaatcc acaatgtaca aaaaacgagc
    10441 cgttacggaa tattttatct acaaaaactg actaaataaa aatttttcac taattgaata
    10501 gtcatagcca gcgatatacg ctatgcgaaa atgcagatgg caatgagatc cactgctttc
    10561 atctccatta acatcccatt acgcttttat taaggagcat tagcatgttc tcaccgcagt
    10621 cacgcttgcg tcatgcagtt gcagatacgt tcgcgatggt tgtttactgt tctgtcgtga
    10681 acatgtgtat tgaagttttc ctctccggaa tgagcttcga acagtctttt tattccagat
    10741 tggtagcgat tccggtgaac atcttaattg catggccata cggtatgtac cgcgatctgt
    10801 ttatgcgcgc ggcacgcaaa gttagcccgt cgggctggat aaaaaatctg gctgatatcc
    10861 tggcttatgt gacgttccag tcaccggtgt atgtggcgat cttgttagtg gtgggcgcag
    10921 actggcatca gattatggcg gcggtcagtt caaacatcgt tgtttcgatg ttgatggggg
    10981 cggtttatgg ctacttcctc gattattgcc gccgactgtt taaagtcagc cgttaccagc
    11041 aggtaaaagc ctgactcttc ttttcgcgac tggcatcgcc agtcgcgtct ttaactggct
    11101 tcgccaaata aaccattcaa ataacgttca agcgcaacac gcgaactaaa gccatgcggg
    11161 ataccgtaat gctcgtgtcg ctcgcctgct aaatagagcg gaaattgctg gtaatgatcg
    11221 caagttttaa tatcggaagc gggttcagcc agtgttgaac tgcgttcttt taatgttgga
    11281 tgaattacca gagcggtacg tcccattcgc gcttcgcgat taacatagac ataattttcc
    11341 ccacggcggt aaccatacgc tttctgggtt accacatcga cagtaaaacc gactttttca
    11401 agtacgcgcg ccacctcgtc tggtcgtaaa tacatatttg atcctcgtta ttatcaaccg
    11461 cgggcctacc ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg
    11521 atgaacctca gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg
    11581 gatcatatgt ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag
    11641 aatgatgtca atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac
    11701 gccaaagggg aagctgaagc cgcacgcagt aaagctcagg cattgctgaa agaaaccagg
    11761 gcgcgaatgc atggtcgtac tcgcgtccag caagccgcac gcgatgccgt tggctgcgcg
    11821 gattcttttg ttcgtgaaag accctggtgt agcgtgggta cagcagctgc ggtaggtatt
    11881 tttatcggcg cactgttaag catgcgcaaa tcgtagtgca aaaatgataa taaatacgcg
    11941 tctttgaccc cgaagcctgg cttcggggtt tctttttgcc tggtgaatca caaaaatccc
    12001 cctaccccgt cacgctcata tccagggtaa tttcgaccac tatttgctat atattgtgtg
    12061 gttgaatctt ttttcaacta catctagtat ctctgtatca acagagatac aacccgacgc
    12121 gtatcatcgc gccgtatctt cattttaaac ggaaatacga atcatgcgca ttactattta
    12181 cactcgtaac gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accggggctt
    12241 tgattttgaa atgattaatg tcgatcgcgt tcctgaagca gcggaagcgt tgcgtgctca
    12301 gggctttcgt cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgtcc
    12361 ggacatgatt aaccgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc
    12421 tacttctcca gcagctccga aaacacgcag cgttttatcg aacgtttagg tctgcccgcg
    12481 gtgcgcatcc cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc
    12541 gtgccctctt acggcggcgg cggtacggct ggcgcggtgc cacgacaggt aattcgcttt
    12601 ttaaacgacg agcacaaccg ggcgttgctt cgcggcgtta ttgcttctgg taatcgcaac
    12661 tttggtgagg cgtatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg
    12721 ctgtaccgtt ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta
    12781 accgaatttt ggcaacgaca accgcagaat gcctgacgca ggaaacgatg gattaccacg
    12841 cgctgaatgc gatgcttaac ctctacgata gcgcaggtcg cattcagttc gataaagacc
    12901 gccaggccgt tgacgccttt attgcgacgc atgtgcgtcc gaacagtgtg accttcagta
    12961 gccagcagca gcgcctgaac tggctggtca acgaaggtta ctatgatgaa agcgttctta
    13021 atcgctactc tcgcgacttt gtcattacgc tgtttgccca cgcacacacc agcggttttc
    13081 gtttccagac attcctcggg gcatggaagt tttacaccag ctatacgttg aagacattcg
    13141 acggtaaacg ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg
    13201 cacaaggcga tgcgacgctg gcgttgcaac tgaccgatga aatgctgtca ggacgctttc
    13261 agccagccac gccaacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct
    13321 gttttctgct gcgtattgaa gacaatatgg agtcgattgg ccgggcggta aattccgcac
    13381 tgcaactgtc gaaacgcggc ggcggcgtgg catttttgct gtcgaatctg cgggaagcgg
    13441 gcgcgccaat taaacgtatt gaaaatcaat cttctggcgt gattccggtg atgaaaatgc
    13501 tggaagacgc attttcctat gccaaccagc tcggcgctcg tcagggggct ggtgcagttt
    13561 atttacatgc ccatcatccc gatattctgc gttttctcga tacgaaacgg gaaaatgccg
    13621 acgagaaaat ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc
    13681 atctggcaaa agagaatgcg cagatggcgc tgttttcgcc ttatgacgta gagcgagttt
    13741 atggcaagcc gtttgccgat gtggccatca gccaacacta tgacgaactg gttgccgatg
    13801 aacgcattcg caaaaaatac ctcaacgccc gtgatttctt ccagcgactg gcagaaatcc
    13861 agtttgagtc cggctatccc tacatcatgt atgaagacac ggtaaaccgt gctaacccta
    13921 tcgccgggcg cataaatatg agtaatctct gctcagaaat tttgcaggtt aacagcgcct
    13981 cagagtatga cgagaatctc gactatgccc gtacaggcca tgatatttcc tgcaatttag
    14041 gttcgttgaa tattgcccac accatggatt cccccaactt tgcccgcacg gtagagactg
    14101 ccgtacgcgg tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg
    14161 aagccggaaa tgccgcctca cacgccatcg ggctggggca gatgaattta cacggttatc
    14221 tggcgcgaga aggcatcgct tatggttcgc cggaagcact ggatttcacc aatctctatt
    14281 tctataccat cacctggcac gcactgcgta cctcgatgtt gctggcacgc gaacgcggtg
    14341 aaaccttcgc cgggttcaaa cagtcacgtt atgccagtgg tgaatatttc agccaatatc
    14401 tgcaaggtaa ctggcagccg aaaacggcga aagttggcga actgtttgcc cgcagcggta
    14461 ttacgttacc tacccgtgag atgtgggcgc aactgcgcga cgacgtgatg cgctacggca
    14521 tttataacca gaatcttcag gcggtgccgc caaccggttc tatctcttat atcaaccatg
    14581 ctacgtcgag tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag
    14641 gacgcgttta ctaccctgcc ccgtttatga ctaacgagaa tctggcgctg tatcaggatg
    14701 cttacgaaat cggcgcagaa aagatcatcg acacctacgc ggaagcgact cgccatgtcg
    14761 atcagggttt gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca
    14821 aagcgcagat ttacgcctgg cgcaaaggta tcaaaacgct ctattacatt cgcctgcgtc
    14881 agatggcgct ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat
    14941 ctatgaaact ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc
    15001 tggaggtgtg gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga
    15061 acgatattcc tgcctggcag acattaactg tcgtagaaca acaactgacg atgcgcgttt
    15121 ttactggcct gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc
    15181 ccgatgcact cacgcctcat gaagaagcgg tattatcgaa tatcagcttt atggaagcgg
    15241 ttcatgcccg ctcttacagt tcgattttct cgacgctgtg tcagaccaaa gatgtcgatg
    15301 ccgcctacgc ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc
    15361 aacattatcg cggtgatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt
    15421 ttttgttcta ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctgacca
    15481 ataccgcaga tctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatag
    15541 gctataaata tcagaaaaac atggaaaaga tatctctggg acaacgtgaa gagttgaaga
    15601 gtttcgcctt cgatttattg ctggaactct acgacaacga gttgcaatac accgatgagc
    15661 tgtacgccga aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacgccaata
    15721 aggctttgat gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc
    15781 cggcaatcct cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt
    15841 caggctcctc ttatgtgatg gggaaagcgg ttgaaacaga agatgaagac tggaatttct
    15901 gagggtgtta ttttcaaaaa tatcactacc cgcagcaggg aaataattcc cgccaaatag
    15961 ctttttatca cgcaaatatt ctgtagtgat ctacactgat actctgttgc attattcgcc
    16021 tgaaactaca atattcaggc gttttttcgc tatctatgac aaaaaatatc aacttttctc
    16081 gatttgctct cagcccttat atcacgggaa attccggcga tttgctcgca tcaatattca
    16141 tgccacattt gccctcaggg gttgcctcag attctcagta tgttagggta gaaaaaagtg
    16201 actatttcca ttgggtaata tatcgacata aacaaataaa ggaatctttc tattgcatgg
    16261 caattaaatt agaaattaaa aatctttata aaatatttgg tgagcatcca cagcgagcgt
    16321 tcaaatatat cgaacaagga ctttcgaaag aacaaattct ggaaaaaact gggctatcgc
    16381 ttggcgtaaa agacgccagt ctggccattg aagaaggcga gatatttgtc atcatgggat
    16441 tatccggctc gggtaaatcc acaatggtac gccttctcaa tcgcctgatt gaacccaccc
    16501 gcgggcaagt gctgattgat ggtgtggata ttgccaaaat atccgatgcc gaactccgtg
    16561 aggtgcgcag aaaaaagatt gcgatggtct tccagtcctt tgccttaatg ccgcatatga
    16621 ccgtgctgga caatactgcg ttcggtatgg aattggccgg aattaatgcc gaagaacgcc
    16681 gggaaaaagc ccttgatgca ctgcgtcagg tcgggctgga aaattatgcc cacagctacc
    16741 cggatgaact ctctggcggg atgcgtcaac gtgtgggatt agcccgcgcg ttagcgatta
    16801 atccggatat attattaatg gacgaagcct tctcggcgct cgatccatta attcgcaccg
    16861 agatgcagga tgagctggta aaattacagg cgaaacatca gcgcaccatt gtctttattt
    16921 cccacgatct cgatgaagct atgcgtattg gcgaccgaat tgccattatg caaaatggtg
    16981 aagtggtaca ggtcggcaca ccggatgaaa ttctcaataa tccggcgaat gattatgtcc
    17041 gtaccttctt ccgtggcgtt gatattagtc aggtattcag tgcgaaagat attgcccgcc
    17101 ggacaccgaa tggcttaatt cgtaaaaccc ctggcttcgg cccacgttcg gcactgaaat
    17161 tattgcagga tgaagatcgc gaatatggct acgttatcga acgcggtaat aagtttgtcg
    17221 gcgcagtctc catcgattcg cttaaaaccg cgttaacgca gcagcaaggt cttgatgcgg
    17281 cgctgattga tgcgccgtta gcagtcgatg cacaaacgcc tcttagcgag ttgctctctc
    17341 atgtcggaca ggcaccctgt gcggtgcccg tggtcgacga ggaccaacag tatgtcggca
    17401 tcatttcgaa aggaatgctg ctgcgcgctt tagatcgtga gggggtaaat aatggctgat
    17461 caaaataatc cgtgggatac cacgccagcg gcggacagtg ccgcgcaatc cgcagacgcc
    17521 tggggtacac cggcgactgc accgactgac ggcggtggtg ctgactggct gaccagtacg
    17581 cctgcgccaa acgtcgagca ttttaatatt ctcgatccgt tccataaaac gctgatcccg
    17641 ctcgacagtt gggtcactga agggatcgac tgggttgtta cccatttccg tcccgtcttc
    17701 cagggcgtgc gcgttccggt tgattatatc ctcaacggtt tccagcagtt gctgctgggt
    17761 atgcccgcac cggtggcgat tatcgttttc gctctcatcg cctggcagat ttccggggtc
    17821 ggaatgggcg tggcgacgct ggtttcgctg attgccatcg gcgcaatcgg tgcctggtcg
    17881 caggcaatgg ttactctggc gctggtatta accgccctgc tgttctgtat cgtcatcggt
    17941 ttgccgttgg ggatctggct ggcgagaagt ccgcgagcgg cgaaaattat tcgtccactg
    18001 cttgatgcca tgcagaccac gccagcgttt gtttatctgg tgccaatcgt catgctattt
    18061 ggtatcggta acgtgccggg cgtggtggtg acgatcatct ttgctctgcc gccgattatc
    18121 cgtctgacca ttctggggat taaccaggtt ccggcggatc tgattgaagc ctcgcgctca
    18181 ttcggtgcca gcccgcgcca gatgctgttc aaagttcagt taccgctggc gatgccgacc
    18241 attatggcgg gcgttaacca gacgctgatg ctggcccttt ctatggtggt cattgcctcg
    18301 atgattgccg tcggcgggct gggtcagatg gtacttcgcg gtatcggtcg tctggatatg
    18361 gggcttgcca ccgttggcgg cgtcgggatt gtgatcctcg ccattatcct cgatcgcctg
    18421 acgcaggccg ttgggcgcga ctcacgcagt cgcggcaacc gtcgctggta caccactggc
    18481 cctgttggtc tgctgacccg cccattcatt aagtaactct gcacttgccc ggtgacgccg
    18541 ggcattatca ccctgccaaa aaaaggaata acaatgcgac atagcgtact ttttgcgaca
    18601 gcgtttgcca cgcttatctc tacacaaact tttgctgccg atctgccggg caaaggtatt
    18661 actgttaatc cagttcaaag caccatcacc gaagaaacct tccagacgct gctggtcagt
    18721 cgtgcgctgg agaaattagg ttataccgtc aacaaaccca gcgaagtgga ttacaacgtt
    18781 ggctacacct cgcttgcttc cggcgatgca accttcaccg ccgtgaactg gacgccactg
    18841 catgacaaca tgtacgaagc tgccggtggc gataagaaat tttatcgtga aggggtattt
    18901 gttaacggcg cggcacaggg ttacctgatc gataagaaaa ccgccgacca gtacaaaatc
    18961 accaacatcg cacaactgaa agatccgaag atcgccaaac tgttcgatac caacggcgac
    19021 ggaaaagcgg atttaaccgg ttgtaaccct ggctggggct gcgaaggtgc gatcaaccac
    19081 cagcttgccg cgtatgaact gacccatacc gtgacgcata atcaggggaa ctacgcggcg
    19141 atgatggccg acaccatcag tcgctacaaa gagggcaaac cggtgtttta ctacacctgg
    19201 acgccgtact gggtgagtaa cgaactgaag ccgggcaaag atgtcgtctg gttgcaggtg
    19261 ccgttctccg cactgccggg cgataaaaac gccgatacca aactgccgaa tggtgcgaat
    19321 tatggcttcc cggtcagcac catgcatatc gttgccaaca aagcctgggc cgagaaaaac
    19381 ccggcagcag cgaaactgtt tgccattatg cagttgccag tggcagatat taacgcccag
    19441 aacgccatta tgcatgacgg caaagcctca gaaggcgata ttcagggcca cgttgatggc
    19501 tggatcaaag cccaccagca gcagttcgat ggctgggtga atgaggcgct ggcagcgcag
    19561 aagtaatttt tattcaggcg gataaggcgt ttttgcctca tccgccgctc tgtacacaat
    19621 gcctgatgcg acgctgacgc gtcttatcat gcctacaaaa tacactcatt ccgtatggcg
    19681 gataaggcgt tttcgccgca tccgccgttc tgtgcacaat gcctgatgcg acgctggcgc
    19741 gtcttatcat gcctacaaac cacatcgcac atttcaacaa gctatctttc atcccatatt
    19801 catcaacatc cgctattatt gatttccagc ttaatcatca cctgatgaac aaaaataatg
    19861 actaaaccta atcatgagct tagcccggcg ctgatcgtgc tgatgtctat cgccaccggt
    19921 ctggcggtcg ccagtaacta ttacgctcag ccattgctcg acaccatcgc gcgtaacttt
    19981 tccctttccg ccagttcggc aggctttatt gttaccgccg cgcaattggg ctatgcagca
    20041 ggtctgctgt ttcttgttcc cctcggtgat atgtttgaac gccgccgcct gattgtctcg
    20101 atgaccttac tggcggcagg cggcatgtta atcactgcca gcagtcagtc gctggcgatg
    20161 atgatcctcg gtacggcatt aaccggttta ttctctgtcg tggcacaaat tctggttccg
    20221 ctggcagcga cgctggcttc accggacaaa cgcggcaaag tggttggcac tattatgagc
    20281 ggtctgctgt tggggatctt gctggcacgg acagttgccg gattgctggc gaatctcggc
    20341 ggctggcgca ccgtcttttg ggttgcttcg gtgttaatgg cactgatggc gctggcatta
    20401 tggcgtggtc tgccacaaat gaaatcagaa acccacctca actacccaca gttgttgggt
    20461 tccgttttca gtatgtttat cagcgataaa attctgcgta cccgcgcgtt gctgggctgc
    20521 ctgacctttg ccaatttcag cattctctgg acctcaatgg cctttttgct tgccgctcca
    20581 ccttttaact acagcgatgg tgtaattggt ctgtttggac ttgcgggagc tgccggagcg
    20641 ttgggcgctc gtccggcggg cggttttgcc gataagggca aatcgcacca caccacaact
    20701 ttcggtctgc tgctgctatt actttcatgg ctggcgatct ggtttggtca cacttccgta
    20761 ctggcgttga ttatcggaat cctggtgctg gatctcaccg tgcagggcgt gcatatcact
    20821 aaccagacgg taatttatcg gatacatcct gatgcgcgca atcgcctgac cgcaggttac
    20881 atgactagct actttattgg cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa
    20941 catggcggtt gggctggcgt ttgtctggct ggcgcgacga ttgccctggt taacttactg
    21001 gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt
    21061 cgtggcttta cacttataag ggtgttaaga agcccatcag tctgataagg ttaagatatt
    21121 cattcagtct atttataata ttaacaatcg ttaagcgtac actctatgga aagccctact
    21181 ccacagcctg ctcctggttc ggcgaccttc atggaaggat gcaaagacag tttaccgatt
    21241 gttattagtt atattccggt ggcctttgcg ttcggtctga atgcgacccg tctgggattc
    21301 tctcctctcg aaagcgtttt tttctcctgc atcatttatg caggcgcgag ccagttcgtc
    21361 attaccgcga tgctggcagc cgggagtagt ttgtggattg ctgcactgac cgtcatggca
    21421 atggatgttc gccatgtgtt gtatggcccg tcactgcgta gccgtattat tcagcgtctg
    21481 caaaaatcga aaaccgccct gtgggcgttt ggcctgacgg atgaggtttt tgccgccgca
    21541 accgcaaaac tggtacgcaa taatcgccgc tggagcgaga actggatgat cggcattgcc
    21601 ttcagttcat ggtcatcgtg ggtatttggt acggtaatag gggcattctc cggcagcggc
    21661 ttgctgcaag gttatcccgc cgttgaagct gcattaggtt ttatgcttcc ggcactcttt
    21721 atgagtttcc tgctcgcctc tttccagcgc aaacaatctc tttgcgttac cgcagcgtta
    21781 gttggtgccc ttgcaggcgt aacgctattt tctattcccg tcgccattct ggcaggcatt
    21841 gtctgtggct gcctcactgc gttaatccag gcattctggc aaggagcgcc cgatgagcta
    21901 tgaggttcta ctgcttgggt tactggttgg cgcggcgaat tattgcttcc gctatttgcc
    21961 gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt ggcgcggtag gtattttgct
    22021 cggcaccatt ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg caccagaagt
    22081 aatgcacgat acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag
    22141 tttctataaa acacgcagca ttatcatccc aacactgctt agtgcgctgg cctatgggct
    22201 cgcctggaaa gtgatggcga ttatataacc cacaagaatc atttttctaa aacaatacat
    22261 ttactttatt tgtcactgtc gttactatat cggctgaaat taatgaggtc atacccaaat
    22321 ggatagttcg tttacgccca ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga
    22381 ttttccttat caggagatcc ttctgactcg tctttgcatg cacatgcaaa gcaagctgct
    22441 ggagaaccgc aataaaatgc tgaaggctca ggggattaac gagacgttgt ttatggcgtt
    22501 gattacgctg gagtctcagg aaaaccacag tattcagcct tctgaattaa gttgtgctct
    22561 tgggtcatcc cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat
    22621 cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg
    22681 tcacgagttt ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc tactctggtc
    22741 cgcgctcagc acaacagaaa aagatcagct cgagcaaatc acccgcaaat tgctctcccg
    22801 tctcgaccag atggaacaag acggtgtggt tctcgaagcg atgagctaac gcgtcatctc
    22861 gctcaaaaat ccagacttat aaaagaaaaa atgactggcc agcatcgcaa catgctggcc
    22921 tttttggcaa gcaggtcggc tcagccgatg agttaagaag atcgtggaga acaatatgag
    22981 cgcaaatgcg gagactcaaa ccccgcagca accggtaaag aagagcggca aacgtaagcg
    23041 tctgctcctc cttctcacct tgctctttat aattattgcc gtagcgatag ggatttattg
    23101 gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca tacgtggcag ggaatcaaat
    23161 tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata acaccgattt
    23221 tgtaaaagaa ggcgacgtgc tggtcactct cgacccgaca gatgctcgcc aggcgtttga
    23281 aaaagccaaa actgcactgg cttccagcgt tcgccaaacc caccagctga tgattaacag
    23341 caaacagttg caggcgaata ttgaggtgca gaaaatcgcc ctcgcaaaag cacaaagcga
    23401 ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca
    23461 cgcccgcgac gccgtcacca gtgcccaggc gcaactggac gtcgctattc aacaatacaa
    23521 tgccaatcag gcgatgattc tggggactaa actggaagat cagcctgccg tgcaacaggc
    23581 tgccaccgaa gtacgtaacg cctggctggc gctggagcgt acccgtattg tcagtccgat
    23641 gaccggttat gtctcccgcc gcgcggtaca gcctggggcg caaattagcc caacgacgcc
    23701 gctgatggcg gtcgttccag ccaccaatat gtgggtggat gccaacttta aagagacgca
    23761 gattgccaat atgcgtatcg gtcagccggt cactatcacc acggatattt acggcgatga
    23821 tgtgaaatac accggtaaag tggttggtct ggatatgggc acaggtagcg cattctcact
    23881 gcttccggcg caaaatgcga ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg
    23941 tatcgaactg gaccagaaac agctggagca atacccgctg cgcattggtt tgtccacgct
    24001 ggtgagcgtc aataccacta accgtgacgg tcaggtactg gcaaataaag tacgttcaac
    24061 accggtcgcg gtaagtaccg cgcgtgaaat cagcctagca cctgtcaata aactgatcga
    24121 cgatatcgta aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac
    24181 cgctggaagg cgcgcaactg gtcattatga cgattgcgct gtcactggcg acattcatgc
    24241 aggtgctgga ctccaccatt gctaacgtgg cgatccccac tatcgccggg aatctgggct
    24301 catcgctcag ccagggaacg tgggtaatca cttctttcgg ggtggcgaat gccatctcga
    24361 tcccgcttac cggctggctg gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca
    24421 ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg aatatgctga
    24481 tcttcttccg cgtgattcag gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc
    24541 tattgctgaa taactacccg ccagccaaac gctcgatcgc actggcgttg tggtcgatga
    24601 cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg ttatatcagc gataattacc
    24661 actggggctg gatattcttc atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac
    24721 tgcaaactct gcgcggacgt gaaacccgca ccgaacggcg gcggattgat gccgtggggc
    24781 tggcactgct ggttattggt atcggcagcc tgcagattat gctcgaccgc ggtaaagagc
    24841 tggactggtt ttcatcacag gaaattatca ttcttaccgt ggtggcggtg gtggctatct
    24901 gcttcctgat tgtctgggag ctgaccgacg ataacccgat agtcgatctg tcgttgttta
    24961 agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg
    25021 gcgctattgt tctgctgccg cagttgttgc aggaggtcta cggttacacg gcgacctggg
    25081 caggtttggc ctctgcgccg gtagggatta ttccggtgat cctgtcgccg attatcggcc
    25141 gcttcgcgca taaactggat atgcggcggc tggtaacctt cagctttatt atgtatgccg
    25201 tctgcttcta ctggcgtgcc tatacctttg aaccaggtat ggattttggc gcgtcggcct
    25261 ggccgcagtt tatccagggg tttgcggtgg cctgcttctt tatgccgctg accaccatta
    25321 cgctgtctgg tttgccaccg gaacgactgg cggcggcatc gagcctctcc aactttacgc
    25381 gtacgctggc ggggtctatc ggcacgtcca tcaccaccac catgtggacc aaccgcgagt
    25441 cgatgcacca cgcacagtta accgagtcgg taaacccgtt taacccaaac gcccaggcga
    25501 tgtacagtca actggaaggg cttgggatga cgcaacagca ggcctccggc tggattgccc
    25561 agcagatcac caaccagggg ctgattattt ccgccaatga gatcttctgg atgtcagccg
    25621 ggatattcct cgtcctgctg gggctggtgt ggtttgctaa accgccattt ggcgcaggtg
    25681 gcggcggagg cggtgcgcac taagtacaac taagccagtt catttgaact ggcttttttc
    25741 aattaattgt gaagatagtt tactgactaa atgtgcagtt cctgcaactt ctcttccggc
    25801 agcgccagtt cttcgttgct gttgatgcgc acgtcacgtt ccagaatgct acgcgcaata
    25861 tcctgcgctt cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagttcc
    25921 gggatctgat tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccaggcatca
    25981 gcaacacgct gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc
    26041 attggcgaga tatcgataat ctctacacca ttaccgttaa gatggttacg cataaaacca
    26101 gcaaacaggt gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg
    26161 cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc
    26221 acccgaactg caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc
    26281 atttagccac ctccggtaat ttttttaaaa attttctgaa ctctttcttc ccaggcgagt
    26341 ctgagtatat gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt
    26401 ggccactcac gagtggcctt tttcttttct gtcaggcgtg tttttccagc cacaccgcaa
    26461 acggttcggt atcagcggct tccatttcct gctgacggcg ttcagacgcc tcgcgctcgg
    26521 ctacaaaatc ctcttcgcgc agaatttcca gcggctcttc acgcagcaga ttacggtagg
    26581 cttctgcaaa tgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac
    26641 gggcagagaa agtcagatcg ggattatcga agcaggcaac cagttcatca cacactttct
    26701 gatacgcttc gccgccgtta atactatcca gcgtttgcgc gacgcgtttc agatcgcgga
    26761 acagatcttt acccacctgc ggtaacggga actgtgcggt ttcgcagccg atacccagcg
    26821 tcagacccgg tttgcgacct tcgaggatca cccggttcca gttaacgcgt gtacaggcaa
    26881 gttcgctact gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga
    26941 ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa cgggttgatg tccagcgaac
    27001 gcacttcaat atattcaatg ccgccacgta acagcgcatc agaaggcgac tcgccgctgc
    27061 gggtaacgcg ttttggacga atcggcgcgt acagttcgtt ttcaatctgc aacacgttgc
    27121 tgttgatttg cagcctctta ccgtctttct caataccaat cttcgcgtac tcttccgatg
    27181 gcgttttgat tgcctgttta aggcccgcta cgtactcgta aagatcgttg aaggtaatac
    27241 caagattgct ttgcgattta ttggtatagc cgagatcgct caaacgaaga gaggtcgcat
    27301 acggcaggta atacataccg cactcggttt tctcaaacgg cagcgacgtt ggttttcctt
    27361 gcaggaaaga agaacaaatc gccggagatg caccaaacag ataaggaatg acccaaccga
    27421 aacgatagta attgcggata acgcggaaat agcccgcaga aattttctct ttggcatcag
    27481 cgcccgagat atcaccgcac ttcgcttgcc agaatgccat tggcaaagag aaattgtagt
    27541 gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacgataca
    27601 gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt
    27661 ctgcgatgta gcatggcata cttaacggcc acatccgctc atcgcccata ttgcgcgccg
    27721 tataacgatg cagatcgcgc ataaaggtca gcatatgttc aatatcacca tccactggtg
    27781 taatgaattc cagcaatgct tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg
    27841 aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc atcagcatta acacgcaaag
    27901 tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg
    27961 ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat
    28021 tagcataact gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc
    28081 cctgaacggt cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa
    28141 agattaccgg tccccacgag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa
    28201 ccacgggcat ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag
    28261 ctttctcttg ccagcgcgat gttttacgca atggaaagaa acgcccaagg ataacgttag
    28321 ttaaccctcc aaggctatta cccattgttg ctgttaaaac taaaacccag ggatgactga
    28381 tcccggaaag caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc
    28441 tgagaaaact actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac
    28501 atccacggcg tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa
    28561 gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg
    28621 cgcgggttta tggtgtttga cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa
    28681 tcccaggtgc gccagcaatg cctcagcgat ggcgctttca ctccccgttc ctacagccat
    28741 tgggcgacga ccatgccaac ttttcaccac ttcaacaaga ggaagcggct cgacactatc
    28801 cagcagcata cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc
    28861 ggcctgattc agctcaataa ttgcctgagc aatacgccag gtgggcgatc cattaagcgc
    28921 aatcattgcc tgaacatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt
    28981 acggtgcgta ggctccgtat ccaggattgt gccatccata tcaaaaatta aacctgcata
    29041 acgctcgtac atggtcttct cgcaagtcga aaaatatgac gctactttag cgtaattgct
    29101 tgattttgtc gctgatggcg aaggagaaga aaattcagga aatggataaa gtagttattt
    29161 agaaatgaga tatttttgaa ggaaattttt gggagaagat ggtgcatccg ggaggattac
    29221 tcactggcgt tcgcccttcg ggccgttgct gacgcaacgt tatcctccct ggtgcctgcg
    29281 gttaataccg ctatctcttc gataccttca ttactgaagg ttacggagaa tatggtgcat
    29341 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc
    29401 tacggatgca tcgggaaaat ttattttact gctgatattg atatcgctac taaagccata
    29461 tcaagtaaga gatggtgcat ccgggaggat tactcgcctt gcggctcgcc cttcgggccg
    29521 ttgctaaagc aacgttatca tccctggtgc ctgcggttaa ttccgctacc tcttcgatac
    29581 cttcattgct gaagatttcg gagaatatgg tgcatccggg aggattcgaa cctccgaccg
    29641 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcatcggg aaaatttatt
    29701 ttactgctga tattgatacc gctactaaag ccatatcaag taagagatgg tgcatccggg
    29761 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
    29821 atgcatcggg aaacttactt tactgcagat tttttgatac cgctactaaa gccgtatcaa
    29881 gtaagagatg gtgcatccgg gaggattact cgccttgcgg ctcgcccttc gggccgttgc
    29941 taaagcaacg ttatcctccc tggtgcctgc ggttataccg ccacctcttc cataccttca
    30001 tcgctgaaag tacgtagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt
    30061 tcgtagccga gtactctatc cagctgagct acggatgcaa atggcggtga ggcggggatt
    30121 cgaaccccgg atgcagcttt tgaccgcata ctcccttagc aggggagcgc cttcagcctc
    30181 tcggccacct caccacacgc ctcttacgag tgcttcgaag aacttgttta tcgctcatcg
    30241 tcgctgcgtg gcgcacatat tactttctga gacttataag tcaaacaatt tttcctacac
    30301 ttttatcgtt tgcacacttc acgttcaatt agtctgtaaa aacgtcaaaa agagtgtttt
    30361 atcaacagaa aaatggaggt ctgacagata gtagtaatgc aaaaaaatgg agacttaagt
    30421 tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag gagaaatttt gagggtgcgt
    30481 ctcaccgata aagatgagac gcggaaagat tagtaactgg actgctggga tttttcagcc
    30541 tggatacgct ggtagatctc ttcacggtga acagaaactt ccttcggggc atttacgcca
    30601 atacgtacct ggttgccctt tacccctaaa actgtcacgg tgacctcatc cccaatcatg
    30661 agggtctcac caactcgacg agtcagaatc agcattcttt gctccttgaa agattaaaag
    30721 agtcgggtct ctctgtatcc cggcattatc catcatataa cgccaaaaag taagcaatga
    30781 caaacacatt acatgtaagc agtcatggca ttacattctg ttaaacctaa gtttagccga
    30841 tatacacaac ttcaacctga ctttatcgtt gtcgatagcg ttaatgcgaa tgccgtgaag
    30901 cgagtccacg gcattgcctg acgcttatat tattgcaatt tcgcgctgac ccagcctttc
    30961 acactggcta acgctgcagg taaggccgca gcatccgtac caccggcttg cgccatgtca
    31021 ggacgtccac cacccttgcc gcccacctgc tgagcgacca taccaatcag ttcccctgct
    31081 ttcacacgat ctgtgacgtc cttagatacg cctgcaatca gagaaacctt accttcggct
    31141 accgttgcca gcacgataat tgtcgacccc agctgatttt ttaaatcgtc aaccatggta
    31201 cgcaacattt tcggctcaac accgctaagc tcgctaacca acagcttaac accattaaca
    31261 tcaattgcct tactggaaag atttgcgctc tcctgtgcgg cagcttgttc tttaagctgt
    31321 tgtagttctt tttctaactg acgcgtacgt tccagtactg agcgcacttt atccgccaga
    31381 ttattgctat cgcctttcag cagatgcgcg acttcgctta agcgatcgct gtctgcatga
    31441 acggtggtga tagcgccttc tccggttacc gcttcgatac gacgaacgcc tgcagcagta
    31501 cccgattcag agatgatgcg gaacagacca atatcaccag tgcggctggc gtgagtaccg
    31561 ccacacagct cggtggagaa atcgcccatg ctcagcacgc gtacacgctc atcatacttc
    31621 tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt cgagatccat gatgttggtt
    31681 tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt cttcgaccgc acgaatctct
    31741 tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca gcaccttgtc gttaaccagt
    31801 gaacctttct gcgatacatg agtccccaga acctggcgca gcgcagcgtg catcaggtgc
    31861 gttgcggagt gattcagacg aatacgggcg cgacgagcct catcaacatc agcctgcaca
    31921 gcgtcgccca ctttcagaga accctcagca agtttaccga tgtgaccaat cgcctggccg
    31981 tatttctgcg tatcttccac cgcgaaggag aagttagcgc ctttcagttc gcctttatca
    32041 ccaacctgac cgccggattc cgcatagaat ggtgtttgat ccagcacgac cacagcttcc
    32101 tggcctgcat tgatggcatc aaccgcttta ccatcaacaa acagcgcggt cactttgccg
    32161 ttcagttcca gatggtcata gcctttaaat tcagatgcac tatcaacacg gatcattgcg
    32221 ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac gacgctgctc ttccattgct
    32281 gcttcaaaac cagcttcatc aactttgatg ttgcgctcac gacaaacatc agccgtcagg
    32341 tcaaccggga agccataggt gtcgtacaga cggaaagccg tttcaccatc cagcgtatca
    32401 ccagaaagtt ttgccagctc ttcatccagc aacgccagac cgcgctccag agtacgggca
    32461 aactgctctt cttcagtctt cagcacctgc tcaacctgcg cctgctggcg tttcaggtct
    32521 tcacccgcag agcccataac gtcgatcagc ggaccaacca gtttgtagaa gaaggtttct
    32581 ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa tgatacgacg cagtacataa
    32641 ccacggtttt cattggacgg cattacgcca tccgcgatca ggaacgcaca agaacgaatg
    32701 tggtcagcga ttacgcgcag cgatttattg ctcagatcgg tcgcgccagt gactttcgct
    32761 accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt tagagttaac gtgttgcagc
    32821 accgcagcaa tacgctccag acccataccg gtatctacag acggcttcgg cagcggttcc
    32881 atcgtgccat ctgcctggcg gttgaactgc atgaagacga tgttccagat ctcaatgtag
    32941 cggtcgccgt cttcttccgg acttcccgga gggccacccc aaatgtggtc gccgtgatcg
    33001 tagaagattt cggtgcacgg gccgcacgga ccagtgtcac ccatctgcca gaagttgtca
    33061 gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa tacgttcgcg cgggatcccg
    33121 acttcttttt cccagatttc gtaggcttcg tcgtcgcttt catagacggt aacccacaga
    33181 cgctctttcg gcagggcaaa ccatttttcg ctggtcagca gttcccatgc aaactgaatg
    33241 gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca gcatttcgaa gaaggtatgg
    33301 tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt taccacccgc acgcacgcag
    33361 cgttgggaag tggtagcgcg ggaataatta cgcttgtcga gcccaaggaa cacatccttg
    33421 aactggttca tcccggcgtt ggtaaacaac aaagttgggt cgttatgggg taccagggag
    33481 ctgctggcaa ctacctgatg tcccttacta tggaaaaagt cgagaaacgc ctgacggatc
    33541 tcagcggtgc tcttgctcat aattatcctg aaatcaagct aacgaaatat cgccaccagc
    33601 tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg ggaataagat aagttttctt
    33661 gactgggaag taaaataccg tatgcgttca gtcggcaaaa tttcgccaaa tatcctggat
    33721 atcttccatc agatagccac gatagagcag aaaacgctgg atcttaactt tttctgaaaa
    33781 gacagttggc aaaggttcac catattttcg cgtcgcctga tcgcgcgcca gtgcgcacca
    33841 gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg cgggaaatac ctttctgatt
    33901 cagttcctga cgaatacgtg caggtccata acctttgcgg ctacggctgg cgataaagcg
    33961 cgcaacaaat cggctgtcat cgagatagcc atgttcatgg caccaggcaa taacgcgctc
    34021 gtaatcttct gccgtagcat caatctcttc tgggccattt ttgcccataa tcggtgccgc
    34081 gagtttacgt cgcagttctt gctcactgtg atcgcgcacc gccagaatgc gtaccgcacg
    34141 atccaacagg cgagcatatg ccgggcgacg ggacgttgat tctgtcatgg catatcctta
    34201 caacttaaaa aagcaaaagg gccgcagatg cgacccaggt tttgctgata aacagattta
    34261 atagtcaatg agtgtgcaga atgttcccgc aggcccaatt cgttagagga tcgaggtgag
    34321 agtaattttt cacgccagtg ctggcctgtt cccctcaccc taaccctctc cccaaagggg
    34381 cgaggggacc gtccgtgcac acattatcta ttatcatcaa taacgaacga ttaaaaatct
    34441 tcgttagttt ctgctacgcc ttcgctgtca tctacagaga aatccggcgt tgagttcggg
    34501 ttgctcagca gcaactcacg tactttcttc tcgatctctt tcgcggtttc cgggttatct
    34561 ttcagccagg cagttgcatt cgctttaccc tgaccgatct tctcaccttt gtagctgtac
    34621 cacgcgcctg ctttctcgat cagcttctct tttacgccca ggtcaaccag ttcgccgtag
    34681 aagttgatac cttcgccgta gaggatctgg aattcagcct gtttaaacgg cgcagcgatt
    34741 ttgttcttca ccactttcac gcgggtttcg ctacccacca cgttttcgcc ctctttcacc
    34801 gcgccgatac gacggatgtc gagacgaaca gaggcgtaga atttcagcgc gttaccaccg
    34861 gtagtggttt ccgggttacc gaacatcaca ccaattttca tacggatctg gttgatgaag
    34921 atcagcagcg tgttggactg cttcaggtta cccgccagct tacgcatcgc ctggctcatc
    34981 atacgtgccg caaggcccat gtgagagtcg ccgatttcgc cttcgatttc cgctttcggc
    35041 gtcagtgccg ccacggagtc aacgacgata acgtctactg cgccagaacg cgccagggcg
    35101 tcacagattt ccagtgcctg ctcgccggtg tccggctggg agcacagcag gttatcgata
    35161 tcgacgccca gtttacgtgc gtagattggg tccagcgcgt gttcagcatc gataaacgca
    35221 caggttttac cttcacgctg cgctgcggcg atcacctgca gcgtcagcgt ggttttaccg
    35281 gaagattccg gtccgtagat ttcgacgata cggcccatcg gcagaccacc tgccccaagc
    35341 gcgatatcca gtgaaagcga accggtagag atggtttcca catccatgga acggtcttca
    35401 cccaggcgca tgatggagcc tttaccaaat tgtttctcaa tctggcccag tgctgccgcc
    35461 aacgctttct gtttgttttc gtcgatagcc atttttactc ctgtcatgcc gggtaatacc
    35521 ggatagtcaa tatgttctgt tgaagcaatt atactgtatg ctcatacagt atcaagtgtt
    35581 ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca gtagcctgac gacgcaccgc
    35641 atcacggtcg ccgctgaagc attcccgccg ggtaatgcct tcaccgcggg cagtggcaaa
    35701 agcaaaccag acggtgccga caggcttctc ttcactgccg ccatccggcc cggcgatacc
    35761 actaatagac acggcataat cagcacgagc cgctttcagt gcgcctatcg ccatttccac
    35821 cacgacgggt tcactcaccg cgccatgctg cgccagcgtc tcttcgcgta cgccgatcat
    35881 ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt tcaaaccagg cggagctacc
    35941 ggcaatatcg gtaatcactt tcgctaccca accaccggta caagactcgg cagttgttac
    36001 ggttgcgcca cgggctttca gcgcctgccc aacctgttca cttaactgca tcagttcact
    36061 gtcagtcatc acaacctctg tcagtcaaaa atttatgccg gacaagatag cactttcgtc
    36121 aggaagatgg tgttggcgtt gcaattttac gaggaggatt cagaaaaaag ctgattagcc
    36181 agagggaagc tcacgccccc ctcttgtaaa tagctactgt actcgcgcca gcgccacggc
    36241 ttgtcctaac tgccagaccg ccattgcgta atgggtgctg tggttgtaac gggtgatggt
    36301 gtagaagttc ggcagaccgt accagtactg gtagccggtg ccaacatcca gacgcagcag
    36361 gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt aaacctgcgg cggcaagctg
    36421 cgaaatgctg tacttagttt tgaagccatt tggcaagcct ggagcctgac cgtttgccat
    36481 taccgcgacc tgatcgcctt tcacccagcc gtgcgctttg aaatagttcg ccacgctacc
    36541 gatcgcatca accggatccc acaggttgat atgcccgtcg ccgctgaaat ctaccgcata
    36601 ttgtttgtaa gacgacggca taaactgtcc gtagcccatc gccccggcaa aggaaccttt
    36661 cagattgagc ggatcgtcct gctcgtcgcg cgccatcaac aggaaggttt ccagctcgcc
    36721 agagaaatac tccgcgcggc gtgggtagtt aaatgacagc gttgccagcg catcgaggat
    36781 gcgagttttc cccatcacgc gcccccagcg ggtttcaacg ccgataatcc cgacgataat
    36841 ttccggcggt acgccataca cctgccacgc gcgattcaac gcatcttcat actgattcca
    36901 gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat tttttgcgat aacgcagcca
    36961 tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt ggtgcctggt tatccatcag
    37021 ccgcagtacc gaatccagac gtttcgcctg ggagagaatt tcctgcaact gctgacgatc
    37081 gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg gcattcgggt tattagcgaa
    37141 atcgccgccc atctgcatca cattgtgctg cggctcaagc aggaagccgc cagacggcgt
    37201 tccggtggtc gtatcagtct cagtaggttt tggcttgctg ctacaggcgg caagcaacac
    37261 aaaaagggga agcaatgtta cataacgacg cttgaacatg aggggtccat ttaacagatt
    37321 caaccagggg caagtatggt aaagcatcac gccccgcaca aggaagcggt agtcactgcc
    37381 cgatacggac tttacataac tcaactcatt cccctcgcta tccttttatt caaactttca
    37441 aattaaaata tttatctttc attttgcgat caaaataaca cttttaaatc tttcaatctg
    37501 attagattag gttgccgttt ggtaataaaa aaataaatcc tgaaggagag aacaatgata
    37561 gaaaccatta ctcatggtgc agagtggttt atcgggctgt tccaaaaggg cggagaggtg
    37621 tttaccggga tggtgaccgg cattcttccg ctgttgatta gcctgctggt tatcatgaac
    37681 gcactgatta attttatcgg tcagcatcgt attgaacgtt ttgctcaacg ttgcgccggt
    37741 aaccctattt cccgttacct actgttaccg tgcattggca cgtttgtctt ttgcaatccg
    37801 atgaccctaa gcctgggtcg ctttatgccg gaaaagtaca aacccagcta ctacgcggcg
    37861 gcctcttata gctgccactc aatgaatggc ctcttccccc atatcaaccc tggcgaactg
    37921 tttgtttatc ttggcattgc cagcggtctg acaacgctga acctgccact tggcccactg
    37981 gcggtgagtt atctgctggt tggtctggtc accaatttct tccgcggctg ggtgaccgat
    38041 ctgaccaccg ccatttttga gaaaaagatg ggcattcaac ttgaacaaaa agttcacctg
    38101 gcaggagcaa catcatgacg catattcgga tcgaaaaagg aacgggtggc tggggcggcc
    38161 cgcttgagct gaaagccacg ccgggaaaaa aaatcgtcta tatcaccgcc ggtacccggc
    38221 ctgcgattgt tgacaaactg gcacagctta ctggctggca ggctattgac ggatttaaag
    38281 aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc ggcacattac
    38341 gctgcggcat ctatccgaaa cgacgtattc ccaccattaa tatccactcg acgggcaagt
    38401 ccggtccgct ggcgcagtac attgtggaag atatttatgt ctctggcgta aaagaagaaa
    38461 acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt gactatgaca
    38521 ccagtaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga atgggcatgg
    38581 ggtccgccgt tgcggtgctg tttcaatctg gtcgtgacac catcgacact gtattaaaaa
    38641 ccattctgcc gtttatggca ttcgtctcgg cgctcattgg catcattatg gcttctggcc
    38701 ttggtgactg gattgcccac ggtcttgctc cgctggcgag ccatccactg ggtctggtca
    38761 tgctggcgct catctgctcc ttcccactgc tttcaccttt cctcggccca ggcgcagtta
    38821 tcgcacaggt tatcggcgta ttgattggcg tgcagattgg tctcggcaat attccgccgc
    38881 atctggcttt accggcactg tttgccatca acgcgcaggc ggcctgcgac ttcatcccgg
    38941 tcggtttgtc gctggcggaa gctcgtcagg acacggttcg cgtcggtgtc ccttctgtac
    39001 tggtgagccg ctttttaacc ggcgcgccga ctgtactgat cgcctggttt gtctccggtt
    39061 ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat cacccgtatc
    39121 ggcgcgagtg ccactgacgc cctcagcgac cagatgctca tcacctttcg tgaaggcgcg
    39181 ccagcggacc tcgaagagta ttgcttcatt cattgccacg gcgagttgaa aggtgaactc
    39241 catcctggtt tgcaatttac gctcgggcag catcgctatc cggtgaccgc cgttggcagc
    39301 gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga tggtttaaac
    39361 gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tacccgacga tatcgcgccg
    39421 ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt tgccgttgtc
    39481 atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg gtctggctgc cgaggggtat
    39541 cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca agaaattaac
    39601 gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg acgccaccag cgagcaaagc
    39661 gttctggcgc tctctcgtgg ggtagatgaa atctttggtc gcgtggattt gctggtctat
    39721 agcgccggaa tagcaaaagc agcctttatc agcgacttcc agctcggcga ttttgaccgt
    39781 tcgctacaag tgaatctggt gggttatttc ctgtgtgccc gtgaattttc gcgtttgatg
    39841 atccgcgacg ggattcaggg tcgcattatt cagatcaact cgaaatccgg caaagtgggt
    39901 agcaaacaca actctggcta cagcgcagcg aaatttggtg gcgtcgggct gactcaatca
    39961 ctggcgctgg atctggcgga gtacggcatt acggtgcatt cactgatgct cggtaacctg
    40021 ctgaaatcgc cgatgttcca gtcactgttg ccacaatacg cgaccaagct gggtatcaaa
    40081 ccggatcaag tcgagcagta ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat
    40141 caagatgtgc tgaatatgct gctgttctac gccagtccta aggcgtcgta ctgcaccgga
    40201 cagtcgatca atgtcaccgg cggtcaggtg atgttctgat caacagcgga gatccgttaa
    40261 ggatctccgt gagactatag aatgcctgat gcgctacgct catcaggcat acaggacttc
    40321 cgccactaca ttaaggaaaa gttatggtat ccgcactcat caccgtcgcc gttatcgcct
    40381 ggtgtgcgca actggcctta ggcggctggc aaatttctcg ttttaaccgt gccttcgaca
    40441 cactatgcca gcaagggcgg gtcggcgtgg gccgttccag cgggcgcttt aaaccgcggg
    40501 tcgtggtcgc catcgccctg gacgatcagc agcgcatcgt cgacaccttg tttatgaaag
    40561 gactgaccgt cttcgcccga ccgcaaaaaa ttcccgcaat taccggtatg catgcgggtg
    40621 atttacagcc cgatgtgatc tttccccatg atccactatc acagaatgct ctatcattgg
    40681 cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta cttgcttgcg aagttatcat
    40741 tttgaaacct aaatcaggta atcacgccca tgaaacctcg tcagcgtcag gccgccattc
    40801 tggagtatct gcaaaagcag ggtaaatgct cggttgaaga attggcgcaa tactttgaca
    40861 ccacaggcac aaccattcgc aaagatctgg tcattctgga acatgccgga accgtcattc
    40921 gtacttatgg cggagtggtg ttgaataaag aggaatccga tccgcctatc gatcataaaa
    40981 cgctcatcaa cacccacaag aaagagctga ttgcagaagc tgccgttagt tttattcatg
    41041 atggcgactc aataattctt gatgctggca gtaccgtttt gcagatggtc cccctgctct
    41101 cgcgctttaa taacatcacg gtgatgacca acagtctgca tatcgtcaat gcgctatctg
    41161 aactggataa cgaacaaacc atcctgatgc caggcggaac atttcgcaaa aaatcggcct
    41221 catttcacgg gcaactggca gagaatgcct tcgagcattt caccttcgac aaattattca
    41281 tgggtactga cggcatcgat ctcaatgcgg gcgtaaccac cttcaacgag gtctataccg
    41341 tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt aatggcggac tcatcaaagt
    41401 ttggccgtaa aagccccaac gtagtttgca gtcttgaaag cgtcgataag ctgattaccg
    41461 acgcaggtat cgatccggcg tttcgtcagg cgctggaaga gaaagggatc gatgtgatca
    41521 taaccggaga gagcaatgag tgaagcacta ctgaacgcgg gacgtcagac gttaatgctg
    41581 gagttgcagg aagcaagccg tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc
    41641 aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg gaattggcaa atcgggccac
    41701 attggtaaga aaatcgccgc aacacttgcc agtaccggca caccggcttt ttttgtccat
    41761 ccggcagaag cactgcacgg cgatctgggg atgattgaaa gccgcgatgt gatgctgttt
    41821 atctcttact ccggtggcgc gaaggaactg gatctgatta ttccgcgtct ggaagataaa
    41881 tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac cgctgggcct ggcggcaaaa
    41941 gcggtgctgg atatctccgt agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc
    42001 agcaccgtca ataccctgat gatgggtgac gcgctggcga tggcggtcat gcaggcgcgc
    42061 ggttttaatg aagaagattt tgcccgctcc cacccagctg gggcactggg cgctcgcttg
    42121 ctgaataaag tgcatcatct gatgcgccgt gatgacgcta tcccgcaggt agcgttagcc
    42181 gccagcgtga tggatgcgat gctggagctc agccgcaccg gtctggggct ggtggcggta
    42241 tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg gcgatttacg tcgctggctg
    42301 gttggcggcg gcgcactcac cacgccagtt aatgaagcga tgacgacagg cggcaccaca
    42361 ttacaggcgc aaagccgtgc catcgacgcc aaagagatcc tgatgaagcg caaaatcact
    42421 gccgcaccgg tggtagatga aaacggcaaa ctcaccggcg caattaactt gcaggatttc
    42481 tatcaggccg ggattattta atccttcaat cccagacgtt tcgccagccg atgcaggttg
    42541 gcgacgtcag tttccagcat tcgcgcgcag gcagcccagt tgtgatgatt ttgtgccagt
    42601 gcctggcgaa tggtttcacg ctggaacgct tctgtcgctt cacgcagatt ttgcttaaca
    42661 acgggcaccg ctgccgcttc tggcggcggc aacgtcacct caggaaaagc aaaatgttgc
    42721 gcctcaagaa tcacttcatc gccgctgcga gtggctctcg acagtactac cgcccgatga
    42781 atagcatgtt ccagttcgcg cacgttcccc ggaaaactgt agtgttgcag taaatttcgc
    42841 gccccggcac ttaataccac gcgggagagc cccagccgca aacgacactg ctcgcagaaa
    42901 taccccgcca gcagaatgac atcatcgccc cgctcacgca gcggcggcac cgaaagtgga
    42961 aacacgctca ggcgatgaaa caagtcagcg cgaaatcgcc ctgccagtac ctcttcgcgt
    43021 aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc gcaaactgcg gtcatcgcca
    43081 acgcgctgaa tatcgccata ctgcaacact cgcagcaact tggcctgcaa agccaacgac
    43141 aactcgccga tctcatcgag aaacagtgtg ccgttatccg ccatttcaaa cttcccgctg
    43201 cggttactga tagcgccagt aaacgcccct ttcacatgcc cgaacagttc actttccgcc
    43261 acactttccg gcagtgcggc acagttgaga tagatcagcg gattcaccgc ccgtggcgag
    43321 gcttcatgaa tcgctttcgc caccagctcc ttaccggttc ccgtctcacc gctgattaag
    43381 acgttgagat cggacgccgc cacaatctca atctcttttt tcaattgcgt catgcctggc
    43441 gataagccaa tcatctgcgt ctgtttcacc gcttcaaacg gcgcggcatc gcccggcagc
    43501 atattctggc tttccagttg ctcaatcagc aacgcattgc ttaacgctcc cgccgccagc
    43561 gcagcaatca gccgtaactc ttcatcgctg aaaacatcga actgatcggg ctgcatcccg
    43621 tcgagcgtca atgcgccaat caggttttgc ccggcaaaca atggcagacc aacgcaggcg
    43681 tgaaccttca gactctcctg cccaggaatc aaaccgtcat agggatcggg caattcgctg
    43741 tctgcaggaa agcgcaccac gtccccggcg cgggcaatcg cttcaagccg tggatgccct
    43801 tccagcgcaa agcgtctacc gagtacatcc tttgccagac cgtcgatggc aagcggaata
    43861 aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc actccagcac ctgacgtagc
    43921 gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg
    43981 atattcgcca gcacatcaac ggaaaaactc atctttgcct cactgtcaat ttgactatag
    44041 atattgtcat atcgaccatt tgattgatag tcattttgac tactcattaa tgagcatact
    44101 tttatttata tagtaaaaac aatcagataa aaaactggca cacaatctgc aattagcaag
    44161 acatcttttt agaacacgct gaataaattg aggttgctat gtctattgtg gtgaaaaata
    44221 acattcattg ggttggtcaa cgtgactggg aagtgcgtga ttttcacggc acggaatata
    44281 aaacgctgcg cggcagcagt tacaacagct acctcatccg cgaagaaaaa aacgtgctga
    44341 tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg
    44401 atctggcgga tatcgattac atcgtgatta accatgcaga agaggaccac gctggggcgc
    44461 tgaccgaact gatggcgcaa attcccgata cgccgatcta ctgtactgcc aacgctatcg
    44521 actcgataaa tggtcatcac catcatccgg agtggaattt taatgtggtg aaaactggcg
    44581 acacgctgga tatcggcaac ggcaaacagc tcatttttgt cgaaacacca atgctgcact
    44641 ggccggacag catgatgact tacctgacag gcgacgcggt gctgttcagt aacgatgctt
    44701 tcggtcagca ctactgcgac gagcatctgt tcaacgatga agtggatcag acggagcttt
    44761 tcgagcagtg ccagcgttac tacgccaata tcctgacgcc gttcagccgc ctggtaacgc
    44821 cgaaaattac cgagatcctg ggctttaact taccagtcga tatgatagcg acctcccacg
    44881 gcgtggtctg gcgcgataac ccgacgcaaa ttgtcgagct gtacctgaag tgggccgctg
    44941 attatcagga agacagaatc accattttct acgacaccat gtcgaataac acccgcatga
    45001 tggctgacgc tatcgcccag ggaattgcgg aaactgaccc acgcgtggcg gtgaaaattt
    45061 tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg
    45121 tgctggtcgg cacttcgacg atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg
    45181 aggagatgac tggtttacgc ttccgtaaca aacgcgccag tgctttcggc tctcacggct
    45241 ggagcggcgg tgcggtggat cgtctttcca cgcgcctgca ggatgcgggt ttcgaaatgt
    45301 cgcttagcct gaaagccaaa tggcgaccag accaggacgc tctggagtta tgtcgtgaac
    45361 acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct gccgcagagc acggtgaata
    45421 cggtggttaa agaagaaacc tctgccacca cgacggctga cctcggccca cggatgcagt
    45481 gcagcgtctg ccagtggatt tacgatccgg caaaaggcga gccaatgcag gacgttgcgc
    45541 caggaacgcc gtggagtgaa gttccggata acttcctctg cccggaatgc tccctcggca
    45601 aagacgtctt tgaagaactg gcatcggagg caaaatgagt aacggcattg tgatcattgg
    45661 ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc aaacaggacg ccactattcc
    45721 attaaccctg attgccgccg acagcatgga tgagtacaac aaacctgacc tcagccatgt
    45781 tatcagtcag gggcaacgtg ccgacgacct tacccgccag acggcgggtg aatttgccga
    45841 gcagtttaat ctgcgcctgt ttccgcacac ctgggtgacg gatatcgatg ccgaagcccg
    45901 tgtggtgaaa agccagaata atcagtggca atacgacaag ctagtgctgg caaccggtgc
    45961 cagcgccttt gtcccgccag tgcctgggcg tgagttaatg ctgacgttaa atagtcagca
    46021 agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga cgcgtgttga ttgtcggtgg
    46081 tggcttgatt ggtagcgaac tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct
    46141 aatcgacaac gctgccagta ttctggcgtc attaatgcca ccggaagtaa gcagccgctt
    46201 gcagcatcgg ttgacggaga tgggcgttca tctgttgttg aaatctcagt tgcaggggct
    46261 ggaaaaaaca gattctggca ttctggcaac gctggaccgc cagcgcagta tcgaagtgga
    46321 tgcggtaatt gcctccaccg gactgcgccc ggaaaccgcc ctggcacgac gcgccgggct
    46381 gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa accagtaatg ccgatattta
    46441 cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg ccgttcctcc agccgattca
    46501 acttagcgcg atggtgctgg caaaaaatct tctcggcaat aacacgccac tgaaactacc
    46561 ggcaatgctg gtgaaaatca aaacgccgga attaccgctg catctggcag gcgaaaccca
    46621 gcgtcaggat ttacgctggc agattaatac cgaacgccag ggaatggtgg cgcgcggcgt
    46681 tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag gatcggatga aagaggcatt
    46741 tggattgttg aaaacgttgc cgatgtaggt gggctactgt gcctaaaatg tcggatgcga
    46801 cgctggcgcg tcttatccaa cctacgggga gcgcatgtgt tggtcacata ctgcgttcat
    46861 gtcgcatcca cctactgtgc ccaaaatgtc ggatgcgacg ctggcgcgtc ttatccgacc
    46921 tacggggacg catgtgtagg ccggataagg cgtttacgcc gcatccggca atggtgtcca
    46981 aatgcaacac gttttatccg ttctggactt cacccgctaa ccaacgcgcc gcagcaataa
    47041 ccccctgccc cagagacaaa ccgccatcac ccgccggtaa actctgtgga aagagcaatg
    47101 tgaaatcagc gagataatgc gccagacgtg cacgcagcaa acggttatga ataaccccgc
    47161 cgctaaatac cagcgtagtg ataccacgca tcgtggcctg ctcacgcatc aacgcggcaa
    47221 aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg attaaccggt gcctgccagt
    47281 tcagccactg ctgccagaaa gtggcgagat ccagttgatt gtccaccagc ggcattgtca
    47341 ccggatgcgt cactccgtgg cacgaagctg cgagtgcctc cagagcacaa gccgcttcac
    47401 cttcataact taacgtggct ggcgcacagc ccagtgccgc cgccacggca tcgaacaacc
    47461 gcccacacga tgacgccagc ggcgcgttaa ttccacgctc aatggcccgc gccagcacgc
    47521 tccagttttg ctgttgcaca cttgctgttt cagaataatt ctgccactcc ggcacaaagc
    47581 gcaggcactg cgccagcagg tttcgccacg gctgcgtcgc cgccaaatcg ccacccggaa
    47641 gcgccactgc aggcaagccg cccaggtgct cacattcgcg atagttcacc cgcagacact
    47701 cgccgcccca caaagcgccg ttctccccca taccgatacc atcgagcgtc aaagcaatga
    47761 catcaccgcc atccagcggc cactgatgct ctgccagaca cgccgctgcg tgggcatgat
    47821 gatgcagcac cgtttgcgtc ggcatattca tttcgctcgc ccactggctg gagacatagc
    47881 ccggatgcac gtcatgcaca acgtattgcg gagtgaaatc gtagatgttt tgcatcaggc
    47941 gtaacgcttc gcgccactgc atctggatgc catcgtcact taaatcgccc agatgctgac
    48001 tcaacaccgc ttgttcaccg cgcaccaggc agaaggtgtt tttcagatcc gcgccaagac
    48061 acagcacagg cggaacattt ttaaagcccg gaggcaaaac cagcgcatcc ggcacatacc
    48121 cgcgtgaacg gcgcagcatt tcgccgcttt cgcgcaccac cgaatcatcc atccgctgca
    48181 cgatgtcgcg gttatgtatc aagaatccgt cggcaatgcc ctgcaaatcc gccagcgcct
    48241 gttcgttgct gatagctggt ggtttaccgc tcaggttgcc ggaggtcatc accagcgggc
    48301 attgcagttc ctgtaacagc aaatgctgga gcgggttcgc aggcaacatt accccgactt
    48361 cgttaaggtc aggggcgata tcatcacaaa gctcaggaac gtatttttta tccaccagca
    48421 caatcggcgc ggcgggcgtg gtaagcaact ggcgcgcagc gtctggtaaa ccgtcagcca
    48481 ctggcaacat gaccgccagc ggtttcgccg ggcgatgttt gcgcgcccgc agtgtcgcca
    48541 ccgcgttact gttacgtgca tcgcaggcaa gatgaaatcc gccaatccct ttgatggcga
    48601 caatgttgcc catttttaac tgtgcgatag ccgcctgtaa tgccgcctct tgttctgcat
    48661 gttcaccatg acttacccat tcaagatgtg ggccacactc cgggcaggcc accggctggg
    48721 cgtggaagcg acgatcgagc gggtcgcggt actctttgtc acaagccgga cacaacggaa
    48781 acgccgccat cacggtaaac gggcggtcgt aaggcatggc gcgaataatg gtgaaacgcg
    48841 ggccgcagtg ggtacagttg ataaacggat aacgataacg ccgttcgcct ggggtattca
    48901 tttcggcaag acaggcaggg caagtagcgg catcgggaac aatttgcgta ttcatggtgc
    48961 cgcctgtgct ctggcgtata gtgaactcgg tgggcagttg tgaccagatg tacggctcac
    49021 gctcgacgct atcaatacgc gccagcggcg ggcagtgctg atgcaattga acaagaaacg
    49081 tttccgggtc ttccagcagc cggacttcta cgccatcgcc gtcattacag acatcgccgt
    49141 gaagatttaa ttgctgtgcc agctgccaga caaacggacg aaaaccgacg ccctgcactt
    49201 tgcctcgaat acgcagttgg acaccgcaag atgtgttttt tgccattgag ttattcccgc
    49261 catcatgaat tgcgtaaccc gccctgccgg acacgactgc gtcgtatccg gcagtctcag
    49321 gtcggcgata ccgccagctc cgtattctac gaatatttcc gggaattcct ttgatgccag
    49381 aacagttcta taagattttt agaacatcag cgccgtacgg cgacgttttt ctgcgctcag
    49441 ttgttcaagt ttattacgat cgacacaaat cagcgcatgg gtcgggcaag ccgccataca
    49501 cgccgggccg tcttcgcgat ggttgcacag gtcgcattta ttggcttcag ctttgtcagc
    49561 ccgtacattc agccccgcgc cgctgttgcg gatcaccgga cgtaccacca cttccatcgc
    49621 gccatacggg caagccacca cgcaggtttt gcaaccaatg caacgttcct gcatcacatg
    49681 aacaaaccct ttatcacggc tgatagcacc attcgggcag acgttagcgc acggtgcatc
    49741 ttcacactga cggcagactg tcgccgtgga aatgttcaca cctttaatga catggattcg
    49801 cggtaaaaag gtttccgggg tcagcgatgc acagtcctga ttttcctgat gagaaaccac
    49861 gcacgccact tcacaggtac ggcaaccaat acatttactc gcgtcagcaa tgatgaaacg
    49921 gttcatcaaa ttctccagca atgacagtta atgcgccgat acattcacaa atcatgccag
    49981 tttttaattt gctgttattt aaggaaatta atttctgtaa tgcaggaaaa aagatgtcat
    50041 cgacactagt gacgatgaca tgtgatgaca atgtttatcg cgaaagggca atgagtgagt
    50101 cgcggcggat cagttttccg ctgaaggttt tcggcggtga gaaatccccg ccatcgagca
    50161 taaaaatcag ccgtccaata atttcctgaa tcatctctgt taccggaatt tttacgctgg
    50221 agagcgccgg aacggtgtag ggggcaattg cgatatcatc gaatccgata actgacacct
    50281 gctctggcac cgctacgccg cgctcgtgca acgctttcat cgcacctatc gccatatcgt
    50341 cgttactggc aactaacgcg ctaaatttag ccccacgttc gagcaacgtt tctacccctt
    50401 cggccccgct ggcgggcgtc catttaccgt tagcgataag tttttcattg agcgcaatac
    50461 catgctgcgc cagcgcgtct ttatacccag caagacgttc aatgctggtg ggggaatcca
    50521 tcgagccggt aaggaaagca atctcctgat gtccggcgtt tatcaactct gccacggcat
    50581 taaagctggt ctgtttatga tcgcaccaga cgctatggct gctgtttttg cgcaggcggc
    50641 gattaagcac cattatcggc tgactgtgcg cgtcaatgat gtcatcgatc tcatccacgc
    50701 ttaaaaaacg cgggtaaatc ataatggcgt cgcagcgcaa atccagcaga tactgaatcg
    50761 cctggcgctc ttcttcggcg ctgtgtttac catctgccaa tagcaactgc cgccctttct
    50821 cttccgccat tcgcgcggca tgaaagagta attcactaaa ataaatgccg tggtaaagcg
    50881 tgttggtcac taccagcccc agcgtctgag tactcttcgc cgacagattg cgcgccagca
    50941 agtttgggcg gtaaccgctc tcttctaccg cctgaaacac gcgatcttta gtctcctggc
    51001 tgacgtagcc attacctgaa agcacgcggg aaacggtcgc ttttgaaacc ccggcgcgct
    51061 tcgccacttc cagcatcgtc gtcatcattt tcatcccttt acacgcaatc aacgcagtgt
    51121 actgcaccgt ttgccgattg tccttgcgca atcggcggga aaaatattca ggtgaccggt
    51181 ttcacaaata taaaaaatga acaattaact ctcttgctta tttagtgaca actattcatg
    51241 attttgtgaa accggtttcc taattccgtt tcagcatcgg catttttccg ttacgtcgac
    51301 tgataacaac tacatctacc ctactgataa caggataaaa tccgatggcc aaaaattatg
    51361 cggcgctggc acgctcggtg atagcggcac tgggcggcgt tgataacatc tcggcagtca
    51421 cgcactgtat gacgcgtttg cgctttgtta tcaaagatga ccagcttatc gacagcccga
    51481 cgttaaaaac catccccggc gtgctcggcg tggtacgtag tgacaaccag tgtcaggtga
    51541 ttatcggcaa taccgtttca caagcctttc aggaagtcgt cagcctgctg ccgggagata
    51601 tgcagcccgc acagcccgtg ggtaaaccca aactcacgct gcgtcgcatt ggtgcgggga
    51661 tcctcgatgc gctgatcagc accatgtcac cgctgatccc ggcgattatc ggcggatcga
    51721 tggtcaaact gctggcgatg atcctcgaga tgagcggcgt gttgacaaaa ggatcgccga
    51781 ccttaaccat tctgaatgtg attggtgacg gtgctttctt cttcctgccg ctaatggtcg
    51841 cggcttcggc cgccatcaaa tttaaaacca atatgtcgct ggcgattgcc atcgcaggtg
    51901 tgctggtaca tccgagcttt attgaactga tggcgaaagc ggcccagggt gaacatgtcg
    51961 aatttgccct gattccggtc accgcggtga aatataccta cacggtgatc ccggcgctgg
    52021 tgatgacctg gtgcctgtca tatatcgaac gctgggtgga tcgcattaca ccggctgtga
    52081 ccaaaaactt cctcaagcca atgctgattg tgttgattgc cgcaccgctg gcaatcctgc
    52141 tgattggccc gattggtatc tggatcggta gcgccatttc ggcgctggtt tacaccattc
    52201 atggttatct gggctggctt tcagtcgcca ttatgggcgc gctgtggcct ctgctggtaa
    52261 tgaccgggat gcaccgcgtc tttacgccaa ccatcattca gaccattgcc gaaaccggca
    52321 aagaagggat ggtcatgccg tcagagatcg gcgctaacct gtcgctgggc ggttcatcac
    52381 tggcggtggc gtggaaaacg aaaaacccgg aactgcgcca gacggcgctg gctgcggcgg
    52441 catcagccat tatggcgggg atttccgaac cggcgttata cggcgtggcg atccgcctga
    52501 aacgtccgct tatcgccagt cttatcagcg gttttatttg cggcgcggtt gccggtatgg
    52561 cggggcttgc cagccactca atggcagcgc cggggctatt taccagcgtg cagttcttcg
    52621 atccggcgaa tccaatgagc atcgtctggg tattcgcggt catggcgctg gcggtggtgc
    52681 tgtcgtttat cctcacactg ttgctcggct ttgaggatat tcctgttgag gaagcgactg
    52741 ccgaggcgcg aaagcatcag agcgtacaac cgaccgtcgc caaagaagta agtcttaatt
    52801 gaggatgaaa atgtcagtat ttccagaagg ttttttatgg ggcggcgcgc ttgccgccaa
    52861 ccagtctgaa ggtgcgttcc gtgaaggtgg caaagggctg accactgtcg atatgatccc
    52921 acacggcgag catcgaatgg cggtgaaact ggggctggaa aaacgttttc agttgcgcga
    52981 tgacgagttt tatcccagcc atgaggcgac ggatttttat catcgttata aagaagatat
    53041 cgccctgatg gcagagatgg gattcaaggt tttccgtacc tcaattgcct ggagccgcct
    53101 ctttccgcag ggcgataaaa tcacgcccaa tcagcagggc attgcttttt accgttctgt
    53161 ctttgaagag tgtaaaaagt acggtatcga accgctggtc acgttgtgcc acttcgatgt
    53221 gccgatgcat ctggtcaccg aatatggctc ctggcgtaac cgcaagctgg tggagttttt
    53281 cagccgctac gccagaacct gctttgaagc atttgatggt ctggtgaaat actggctaac
    53341 cttcaatgaa atcaacatta tgttgcatag cccgttctcc ggcgcgggtc tggtgtttga
    53401 agaaggtgaa aatcaggatc aggtgaaata tcaggccgcg catcaccagc tggttgccag
    53461 tgcgctagcc accaaaatcg cccatgaggt taacccgcaa aatcaggtgg gctgtatgct
    53521 ggcgggcggt aacttctacc cttacagctg caaaccggaa gatgtctggg cggcgctgga
    53581 gaaagatcgg gaaaacctgt tttttatcga tgtgcaggcg cgcggcgcgt atccggctta
    53641 ctctgcccgc gtatttcgcg aaaaaggggt aaccatcaac aaagcaccgg gcgatgatga
    53701 aatcctgaaa aacaccgtcg attttgtctc tttcagctat tacgcctcgc gctgcgcctc
    53761 ggcggagatg aacgccaaca acagcagtgc ggcgaacgtg gtgaaatcgc tgcgtaatcc
    53821 gtatctacag gtgagcgact ggggctgggg aattgatcca ctcggtctgc gtatcaccat
    53881 gaatatgatg tacgatcgtt atcagaagcc gctgtttctg gtggaaaacg gcctcggcgc
    53941 aaaagatgaa cttgctgcca atggcgagat taacgatgac tatcgcatca gctatttacg
    54001 cgaacatatc cgcgcaatgg gcgaagcgat tgcagatggc attccgctga tgggctacac
    54061 cacctggggc tgtattgatt tagtttccgc ctctacgggt gaaatgagca aacgctacgg
    54121 ttttgtctac gtagaccgtg acgacgcagg caacggcacg ctgacgcgca cgcgtaagaa
    54181 atcgttctgg tggtataaaa aagtaattgc cagtaatggg gaagatttag agtaagtaac
    54241 cgtgccggat gcggcgtgaa cgccttatcc ggcctacatg tcgcacgaac catgaaggcc
    54301 cgataagcgc agcgcatcgg gcaatctggg gtttggcatc agtcaaaatc aaagaaacca
    54361 ttccggctta acgccaaatc gggctgaaag tgccttaatg tgggatatcg tcagggatcg
    54421 ttgtccagac aaaatctgac tgaccaggga tttagagccg atttcgttct tcaaatccgc
    54481 ataggtcaga ttatgttgat caatcaacgt gcgaagtaac gccacgccaa ccggcattgc
    54541 cgcaacggct ttgtcgaact ctgcaaactt ttcgttgttg ttttcatact ctgcaatgcg
    54601 gctcgccaga aagtcgataa gtggattggt gtcatcttct tcaatcaaat attcgaccag
    54661 ttccagtgct tcacgataat catcttctga gtcactgcct cccagaaagg ggactgcatt
    54721 gaccagttct cttgttgctt taacagcgcg tgcagcatta gcagtcattc tttgttctct
    54781 ctgtagtaac gtgacgtctg tctgcacgta atccccctca tagccactct gcaaaaagtc
    54841 agttctggtt caccccataa agcacatccc cctttatcaa gcaacgctgc gtctgcgcca
    54901 gatcttccgt ttgcgggtcg cgcccggcga tgggtttgag gacgagttta cggttggggg
    54961 tataagtaaa acgcgtgagc gcgggatagc ttaacttgcc aaaccagccg tgaacacgct
    55021 gccatttggc gtcttttacc gatacagact ggttatccag taaccggaat caaactgcat
    55081 atccagcaat tgcgtgtggc cattcctttg taaatccagc aaatcatcaa tgccagtttc
    55141 acttgcggta taaaaaccat cggactgaaa aacacacggg cgaccttgct gattaaaggt
    55201 catcagaatc aaaaacgcgt taggtgccag accatttcct gaaatacccc gccagataac
    55261 cagatcctga atgccgttac gatcaagatc ggcacggtaa agttgcgcat tacccgccat
    55321 attttgcagc actcccacag gcacagacca ggatttaccg gcgcgatctt taccgttcaa
    55381 ttgccattca ctatcatcat ttggcatctt ttcaagcgtc acgccagcaa cgccgacttt
    55441 tctaaaatgc cagttttgct cggcatcata aggcatggtc aggagaggtt tcagcgcccg
    55501 cttttgcgcg tattcgctgt cactcattgg cagatattcg gtaaagctgg tggcctgtgc
    55561 aggcagcact aagctcaata aaaaagcgca catccctgtt ctgatgttca attccctgaa
    55621 ttccctgatg gagaattatt cttcttccac cgctaactgc gcaaatccac catttccttc
    55681 ccagccttcc agtcgttgat aaacggtttc taccgcatct ttaatcggct gggtcatcgg
    55741 gtagtaaaag ccgacgatat ccggctgaat gccgaggaaa atcacttcac caatgtcttc
    55801 ttttaactgg tcgataaggt aattcaacgg catgttatgg gtagtcatca taaacatctc
    55861 ggcgatatca tccgggtcga tgatgcggat ttcgccgggg tttagcccca tatccgtggc
    55921 gtcgacaatc agcagtcgtg tcgggcgcag ttcacggatg gcgacgatgt cgttttccgg
    55981 tgcactaccg ccgtcaatca ccacccagtt acctttcggc gcggcggcgc acttttccgc
    56041 cagcagtggg ccagcgccat catcgcccat catgctattg ccaacacaga gtaaaacgtc
    56101 agtcacgcag tctcctcacc atcaaataga tggcgttttc ctgatgaata tcatgcagca
    56161 tacttaacag cattgtactc cattccttct gctgcgaagt ttgcgctgcc cgggctttat
    56221 cgaatgcgtt ggccagcatc ggcacatggt taatgtcgat gacgatctcg ccatatttcg
    56281 gtacgccttc cattttgcgg cgggcttcgc tgcccgcctc cagagtggca atccatgcca
    56341 gatattcatc ccatgggcag gtgagcgccg cttccaggca atcgataacc ccaaggtggt
    56401 gaccaatcgc caggctgtaa tagaccacct gctgcgcctc ggcgggcgtg gcatcgttct
    56461 catcaataaa tttacggctc agttgactga acaccacctt ttcactcatc ggatacgcgc
    56521 ctcttcaaca acatgattca gatggctgac aatctcgttc agacgcggat cgttttccgc
    56581 ttccagccag cgtgcaacct gttcttcgcc ctgccctaac tgtgtcagga agtcatcggc
    56641 aatctgacga ccgtaacgat aacctgccag gcggcgtgct tcgcgatcca ctttcacacg
    56701 cagcggctgc accatatcgc catgcaggat ttccgccggt tgttcatcct gctcacccgg
    56761 gccacgggcg tgaattttct gctccagcag gccgagcgcc attgcaaagc cgtacagcgt
    56821 ggcggcaggc gttggcgggc aaccagggat gtagacatct accgggacga ttttatccgt
    56881 accgccccac acgcagtaga gatcgtggaa gatcccgcca ctgttaccgc aggcaccgta
    56941 ggagatacag attttcgggt ccggcgcgga ctgccacgca cgcagcgcag gggatcgcat
    57001 tgcacgggtg accgcgccgg taaacagtaa aatatccgca tgacgcggtg aaggaacgac
    57061 tttaatgccg aagcgttctg catcaaacag cggcgaaagc gtggcgaaaa tttcgatttc
    57121 gcagccgttg cagccgccgc agtccacgcg gtaaacgtag gcagaacgtt tgattttttt
    57181 cagtaacgac gccttcatgc tggcgatgga ttcatccacc gtcatcggga ccggaatgcc
    57241 gttggcgtca cgggggccta ataaattgct catcagatgg cctctttcat atggcgagtc
    57301 agttcaatac ggtcggacgg caccaggcat ttctggcgct tacattccgg gcaagtctca
    57361 aagctttcgc ggtggttttc cgcgcggctg tcgccgttgt gcttaagtag cgcaatggcg
    57421 tagtcgatct ctttctggac agcgaaagga cgattgcaga cgcgacagtt gcacagcgcg
    57481 aagcgggact gttgcaggaa gtcttctttc ttccacaccg ccagttcgta ctcttgcgac
    57541 agtttgatcg ccgccgtcgg gcaaacttct tcgcagcgtc cacagaagat gcagcgtcca
    57601 agattgaact cccacgcaag ctctcctgtg gcgaggtcgg tttcaaccgt taaggcgttt
    57661 gacgggcagg cattgacgca ggccgcgcag ccgatgcact gctgcgggtt ctgctctggc
    57721 ttaccacgga agtttttatc aaccgcaatc ggctccagcg gataagacga ggtcgccgtg
    57781 ccggttttga taactttttt gataaaggta aacatggcga ttccttattt cagcggcgag
    57841 tttttacgct caatgctgta gcgctcgagt tctttgtacg gcaccacttt gctcttcttc
    57901 ttacgtacat cgaccaccgt catgcggtcg gtacaggagt agcaagggtc caggctaccg
    57961 ataatcagcg gcgcatcgga aacggtattg ccacgcagca tgtagcgcag ggtcggccag
    58021 ttcgcgtagg tcgcggcacg gcagcgccag cggtacagct tctggttgtc gccggtcatg
    58081 ctccagtgga tatcgtcgcc gcgcggcgct tcggcaaagc ccagcgcgaa acggtgcgga
    58141 atgtaggtaa agccttccac catcagcgga ccgcccggca ggttatccag accgtagtcg
    58201 atcatgttca gcgcggtata aacttcgttg atacgcactt tcagacggga aataacgtcg
    58261 cagccctgct cgctgtggac ttccattggc agcaggccat aaccgacaaa cgggtgatcg
    58321 gcgcgagtat cgcgggcgtg accgctggcg cgaaccatcg ggccaacgtt actgaaatcg
    58381 cgggcgattt ccgggtccag acgaccaatg ccgacggtgc gctgttccat gttcggcgta
    58441 ctcagcagca catccaccag ctcctgcact tcacgacgca tctgttgcgc cagctggcgg
    58501 gtctggatca tgtcatcttt caacagatcg cgacgaatcc cgccgatcag gttcaggccg
    58561 taggttttac gcgcaccggt gaggatctct gccattttca tggaggtttc acgcacgcgg
    58621 aagaactgca taaagccgga gtcaaagccg gtaaagtggc aggcgagacc gaggttgagc
    58681 agatgcgagt gcaggcgttc cacctccagc agaatggcgc ggatcatctg cgcacgttct
    58741 ggcacctgaa tacccatcgc gttttctacc gacgtggtgt aggcagtgct gtgggcaaag
    58801 ccgcagatcc cgcacacacg gtcagagagg aaggtcactt cgttgtaacc catacgggtt
    58861 tccgccagtt tttccatgcc gcgatgaacg tagaacagac ggtagtcggc gtcgataatg
    58921 ttttcgccat cgacgaacag acggaagtgg cccggttcgt cagaagtgac gtgcagcgga
    58981 ccaatcggca cgacgttgtt tttcttgtcg cccagttcgt tgatgaactc gtaggtttca
    59041 gcatcggtag tcggtgccgg acgctgacga taatccatgc tgtctttacg cagcggataa
    59101 agttcatccg gccagtcatc cggcagcacc agacgacgtt catccggcag accaaccgga
    59161 atcaaaccgt acatatcgcg cacttcgcgc tcgccccaca ccgccgccgg aacgcgcggc
    59221 gtcacggacg gatactccgg tttgttggcg tcaacttcga cgcgaaccgt tacccaacac
    59281 ttggtgccct tctccatcga cagcacgtaa taaacggcgt aatgaccatt cagtttgcgt
    59341 tcgtcgttac caaacagcac cgacagccag ccaccctgct tgtagtagag aaactccacc
    59401 acttccggca ggtagttcac cttcacggtc acggtcagct gatctttggt ctgccaggcg
    59461 tggtccagca cgacgcccgg aaatgcctca ttcagcgcgg cgagataatg ttgacctaat
    59521 ttttcttcag acatgctcaa actctcttta atcacgccgc cagcaaggag acgaacgcta
    59581 aaaatgcaaa gccaaaccca gcccaggtaa tgcgcggagt aatatcaaga cgcagacggg
    59641 ccatgctgtt ttcgaacagc gcgataacca ggacgccgac gaccagtttg acgatggcaa
    59701 tcaccagcgc cagcagcagc ccaccggcgg tgaaggtttc catctgtccc cacgggataa
    59761 atacgccgac gaacatctgc agcaccacca gctgtttcag gctgataccc catttcatga
    59821 cgccaaagcc gctgccgctg tattcagaga gcgggccttc ctgcagctcc tgctcggctt
    59881 ccgccaggtc gaacggcagt ttgcccattt cgataaaggt ggcgaacgca caggcacaaa
    59941 gcgccagtac cagcgggatg ctctggctca gcggccagtg ataaacggtg tcggtgatgt
    60001 tgctgatgtt ggtggaaccg gcaacctgtg cggcaaccca cagaccaagc agcagcatcg
    60061 gttcgaccag cacgccaagc atcgcttcac ggctcgcgcc gatagcggta aacgggctac
    60121 cggtatccag accagaaatg gcaaagaaga aacgcgcgat ggcaaagaga tacagtaagg
    60181 tgattaaatc acccagttgc ggcagcggag aaccgacggt caccaccggc agcgcagtag
    60241 cgatagtcag catgacgccc accatcacat acggcgtcag gcggaacacc cagccggagg
    60301 catccgggcc aacgctctgg cggcccagca gtttgataat gtcacgatac tcctgcaaca
    60361 cacccggccc acgacggtta tgtaagcggg ctcgcgccac gcgggtaata ccggagagca
    60421 gcggcgcaac ggcaaatagc accagcgcct gaattaacgg atataaaaca ctcattctca
    60481 ggctcctcgt gaaacaataa tcaccaccag caccgccagt tcaaccagcg ccatccggcg
    60541 gaacagcaac gcactcccct cgcactgcca gcccggcacc agagacaccg gattcagcca
    60601 tttgcgtagt ttcagcaccg gcgcaaacgc ctgtttcacc ggcatggcaa aaccgtgagc
    60661 ggtaatcacc attgattttt cgtggtcgta gccgcacacc caggccgcac cgcgggaacg
    60721 cgacggcaaa cgatcgcctt tgcaaatcgc cataatgatg aatggcagca gcgggcaggc
    60781 aatcagcagc aacgtgatca tcggttgaga aacggtggtg ttagcaggct ccagcggcag
    60841 aggtacagca gcagagagca tcggcagcag ccacggcgca gcaacaccgc caattacgca
    60901 gcaaatcgcc agtgccacta cgcttacgct catcaggagc ggcgcacagg tggcgttttc
    60961 ggcttctttg gtacgcggcg cgccgaggaa cgtgacgcca tagactttcg ccatacacat
    61021 caccgccagc gcaccggtaa ttgccagacc cacagcgagc agcggcccca gcagacgggc
    61081 aacaaacgcg ccactattgc tcagtttgaa aaaggattga tagataaccc attccccggc
    61141 aaacccgttc agcggcggca gcgcagccat tgccatcaac ccgactaaca tggcgatgga
    61201 gataaccggc attttcttgc caataccacc gagtttttcg atatcgcgat gaccggtacg
    61261 gaaccagacg ctccccgccc cgaggaacag tacgcttttg aacaggctat ggttaagcag
    61321 atggtacaga ccaccgacca gaccaagggc aatcagcgcc ggttgttcga gcgcgatacc
    61381 cgttacgcca gcgcccagcc ccagcaggat aatgccgata ttttccaggg tgtggtaagc
    61441 cagcaggcgc tggatgttgt gttccatcaa cgcatacaga ccaccgacga acgcggtgat
    61501 catgccgagc accagcagcg cgatccccca ccacagcggc gcgttaccac ccagcagcga
    61561 cagggttaaa atgcccagca ggccaatttt catgactacc gtagaaaaca acgcggcagc
    61621 tggcgcagag gcgttagcat gtgcctgcgg cacccagccg tgcagcggaa taatcccggc
    61681 cagcaggcca aagccaatca caccgagcag ccagatatcg gaaccgagcg gcagctgttg
    61741 catacgcata tccagcaggc gcagatccag cgtgccataa cgctgccaca gcagccagca
    61801 agcaatcgcc agcagcagag tgccaagacg ccccagcgca aaccacagtt tgccctcttt
    61861 gctgttgctg gtgaggaaca ccgcacacaa ggccatgact tcggccatta ccacgaacat
    61921 gccgaggttg ctggcaatga cggcgcagac ggcggcagcc atcaacatat tgatctgcaa
    61981 gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg ttatagaggc tgacaaacag
    62041 accgcaaagg ccgagggtaa tcagccaaat cgcgttaagc ggggagattt gcacatcgta
    62101 gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca gtcagcacag tgaagcccgc
    62161 ggctgccgta tacaggctac caaccgcgcc gccaattccg gctatccagc cacttagcgt
    62221 cttttgaaaa gaaaagagaa atgccagaac agcggcggca ataaaccacg ccacgccgct
    62281 attgatcagg gaaattgcgc tcatttagcc tctccacttt gagcctgctg aaacaaggtg
    62341 agatcgccaa agtccgtatt aaaagtcagc tcacgcttac gtttgctgac gcgggcgata
    62401 tcggtgttat ccaccagatg cagggctttg gtcgggcaca tccgcacgca ggccggacct
    62461 tgttcatcaa aactgcaaag gtcacatttg acggcgatcg cgcgaatacc cggcacccag
    62521 tcaagcagcg tactgatacg cgccggagca ggcggtgccg gtggcgcttt cggggtattg
    62581 gcatttgcag gaatatccag cggacggctg ccggaaaatt caattgcgcc aaacgggcag
    62641 gcgatgccgc acagcttgca gcttacgcac aggctttcat tcaactgcac ggccccatca
    62701 acgcgggtga tggcgttaac cgggcagacc accgcgcagg gtgcatcttc acagtggtga
    62761 cagagctgcg gcgcagattc tttttcattt agcatcactc tcaggcgcgg cattgattgc
    62821 aggccgtgct ggcgatgcgt ctctgaacag gcggcctcac aagtgtggca gccgatacag
    62881 agcgtggagt cagcaattac aaaacgattc accaggcatt cctcaggtga ttgtcatttt
    62941 tgacgaaaac atgccgatga aatgtcattt tcgacactca tcgacacgcc catccccaaa
    63001 caggcgtaac gcctgcaaaa cgggcaaagc ctcagctcat gctgccgggc tttgtccctt
    63061 taccagttgg cttaaattca ccggcacatt gttttcaatg gcggtgtata agctgtcgta
    63121 aacgccagcg attttttcga gatagtgttc cagaacctgt tcgcgatcgc ggatgttgac
    63181 cttgccatca atcatgccgt caatgctcag ttgcgcctgc gcaatccgct gtttgtcttc
    63241 gccatctttt gtttcgacgt aatactcgat ggtgtggctg ttaaagccat ccgccagggt
    63301 gacgtaaata cgaaaatgtt caaaaaggac gaaacagagt tctttgtccg gcgcgcagct
    63361 catggcgtga tgcaggcgcg ctttcgataa cgtgatcccc tgaaccagcg aattgcagta
    63421 gatgtgccac tggtcctgta ggcgacgatg ccgctgtgcg atgtaatcgg ctttctcgct
    63481 tatttcccaa atagtcattg tcaggttacc cgtttaacag agatgccagc tttaagcatt
    63541 ttttgtgcca acttttaatt tattgttatt aaagagattt ttaagctaaa gatgaatttc
    63601 gtcgccgtgt cgacgtgtca tttcgacatc atcgacatta ttcaccgcag ggataatcaa
    63661 cactggcaca attattgctt gtagctggca atagttaatg ggaggcgata tgcacgaaat
    63721 aaccctctgc caacgggcac tggaattgat cgaacagcag gccgcaaaac acggcgcaaa
    63781 acgcgtaact ggggtctggc tcaaaattgg cgcattttct tgtgtcgaaa ccagctctct
    63841 tgccttttgt tttgatctgg tttgccgcgg cagcgtggcg gaaggttgta aactgcacct
    63901 cgaagaacaa gaggccgaat gctggtgtga aacatgccaa cagtatgtga cgctactgac
    63961 ccagcgcgtc cgccgctgtc cacagtgtca tggtgacatg ctacagattg tggcagacga
    64021 cggtttacag attcggcgga tagaaataga ccaggagtga gcgatgtgta caacatgcgg
    64081 ttgcggtgaa ggcaacctgt atatcgaggg tgatgaacat aaccctcatt ccgcgtttcg
    64141 tagcgcgcca tttgccccgg cggcacgccc gaagatgaaa atcaccggca ttaaagcgcc
    64201 tgaatttacc cccagccaga ctgaagaagg cgacctgcat tacggtcatg gcgaagcggg
    64261 cactcacgca ccgggcatga gccagcgtcg gatgctggaa gtcgaaattg acgtgctgga
    64321 caaaaacaac cgtctggctg aacgcaaccg cgcgcgcttt gctgcccgca agcaactggt
    64381 gctcaacctg gtttccagcc ctggttccgg taaaaccacc ctgctgacgg aaaccttaat
    64441 gcgcctgaaa gacagcgttc cgtgcgcggt tattgaaggt gaccagcaaa ccgtgaacga
    64501 tgccgcacgc attcgcgcta ccggcacacc agcgattcag gtgaacaccg gtaaaggctg
    64561 ccatcttgac gcacagatga ttgccgacgc cgcaccgcgt ctgccactgg acgataacgg
    64621 tattctgttt atcgaaaacg ttggcaacct cgtatgcccg gccagcttcg atctcggtga
    64681 aaaacacaaa gtggcggtgc tttccgttac cgaaggtgaa gacaaaccgc tgaaatatcc
    64741 gcatatgttt gccgccgcct cgctgatgct gctcaacaaa gttgacctgt tgccgtatct
    64801 caactttgac gttgaaaagt gcatcgcctg cgcccgcgaa gtcaatccag aaattgaaat
    64861 catccttatt tccgccacca gcggcgaagg gatggaccag tggctgaact ggctggagac
    64921 acagcgatgt gcataggcgt tcccggccag atccgcacca ttgacggcaa ccaggcgaaa
    64981 gtcgacgtct gcggcattca gcgcgatgtc gatttaacgt tagtcggcag ctgcgatgaa
    65041 aacggtcagc cgcgcgtggg ccagtgggta ctggtacacg ttggctttgc catgagcgta
    65101 attaatgaag ccgaagcacg cgacactctc gacgccttac aaaacatgtt tgacgttgag
    65161 ccggatgtcg gcgcgctgtt gtatggcgag gaaaaataat gcgttttgtt gatgaatatc
    65221 gcgcgccgga acaggtgatg cagttaattg agcatctgcg cgaacgtgct tcacatctct
    65281 cttacaccgc cgaacgccct ctgcggatta tggaagtgtg tggcggtcat acccacgcta
    65341 tctttaaatt cggcctcgac cagttactgc cggaaaacgt tgagtttatc cacggtccgg
    65401 ggtgcccggt gtgcgtactg ccgatgggta gaatcgacac ctgcgtggag attgccagcc
    65461 atccggaagt catcttctgt acctttggcg acgccatgcg cgtaccaggg aaacagggtt
    65521 cgctgttgca ggcaaaagca cgcggtgccg atgtgcgcat cgtttactcg ccgatggatg
    65581 cgttgaaact ggcgcaggag aatccaaccc gcaaagtggt gttcttcggc ttaggttttg
    65641 aaaccaccat gccgaccacc gccatcactc tgcaacaggc gaaagcgcgt gatgtgcaga
    65701 atttttactt cttctgccag catattacgc ttatcccgac gcttcgcagt ttgctggaac
    65761 agccggataa cggtatcgac gcgttcctcg cgccgggcca cgttagtatg gttatcggca
    65821 ctgatgccta taattttatc gccagcgatt ttcatcgtcc gctggtggtg gctggtttcg
    65881 aaccgcttga tctactgcaa ggcgtggtca tgctggtgga gcagaaaata gcggcccaca
    65941 gcaaggtaga gaatcagtat cgtcgggtgg taccggatgc cggtaacctg ctggcgcaac
    66001 aggcgattgc cgatgtgttc tgtgtcaacg gcgacagcga atggcgcggc ttaggcgtga
    66061 ttgaatcttc tggtgtacac ctgacgccag attatcaacg attcgatgcc gaagcgcatt
    66121 tccgcccggc accgcagcag gtctgcgatg acccgcgcgc acgttgtggt gaagtattaa
    66181 cgggcaaatg taagccgcat caatgcccgc tgtttggtaa cacctgtaat cctcaaaccg
    66241 catttggtgc actgatggtc tcctccgaag gagcgtgcgc cgcgtggtat cagtatcgtc
    66301 agcaggagag tgaagcgtga ataatatcca actcgcccac ggcagcggcg gccaggcgat
    66361 gcagcaatta atcaacagcc tgtttatgga agcctttgcc aacccgtggc tggcagaaca
    66421 ggaagatcag gcacgtcttg agctggcgca gctggtagcg gaaggcgacc gtctggcgtt
    66481 ctccaccgac agttacgtca ttgacccgct gttctttcct ggcggtaata tcggcaagct
    66541 ggcgatttgc ggcactgcga atgacgttgc ggtcagtggc gctattccgc gctatctctc
    66601 ctgtggcttt atcctcgaag aaggattgcc gatggagaca ctgaaagccg tagtgaccag
    66661 catggcagaa accgcccgca cggcaggcat tgccatcgtt actggcgata ctaaagtggt
    66721 gcagcgcggc gcggcagata aactgtttat caacaccgct ggcatgggcg caattccggc
    66781 gaatattcac tggggcgcac agacgctaac cgcaggcgat gtattactgg ttagcggcac
    66841 gctcggcgac cacggggcga ctatccttaa cctgcgtgag cagctggggc tggatggcga
    66901 actggtcagc gactgcgcgg tactgacgcc gcttattcag acgctgcgtg acattcccgg
    66961 cgtgaaagcg ctgcgtgatg ccacccgtgg tggtgtgaac gcggtagttc atgagttcgc
    67021 ggcagcctgc ggttgtggta ttgaaatttc agaagcggca ctgccggtta aacctgccgt
    67081 gcgcggcgtt tgcgaactgc tgggactgga cgcccttaac tttgccaacg aaggcaaact
    67141 ggtgatcgcc gttgaacgca acgcggcaga gcaagtgctg gcagcgttac attcccatcc
    67201 actgggaaaa gacgcggcgc tgattggtga agtggtggaa cgtaaaggtg ttcgtcttgc
    67261 cggtctgtat ggcgtgaaac gaaccctcga tttaccacac gccgaaccgc ttccgcgtat
    67321 atgctaataa aattctaaat ctcctatagt tagtcaatga ccttttgcac cgctttgcgg
    67381 tgctttcctg gaagaacaaa atgtcatata caccgatgag tgatctcgga cagcaagggt
    67441 tgttcgacat cactcggaca ctattgcagc agcccgatct ggcctcgctg tgtgaggctc
    67501 tttcgcaact ggtaaagcgt tctgcgctcg ccgacaacgc ggctattgtg ttgtggcaag
    67561 cgcagactca acgtgcgtct tattacgcat cgcgtgaaaa agacaccccc attaaatatg
    67621 aagacgaaac tgttctggca cacggtccgg tacgccgcat tttgtcgcgc cctgatacgc
    67681 tgcattgcag ttacgaagaa ttttgtgaaa cctggccgca gctggtcgca ggtgggctat
    67741 accccaaatt tggtcactat tgcctgatgc cactggcggc ggaagggcat atttttggtg
    67801 gctgtgaatt tattcgttat gacgatcgtc cctggagcga aaaagagttc aatcgtctgc
    67861 aaacatttac gcagatcgtt tctgtcgtca ccgaacaaat ccagagccgc gtcgtcaaca
    67921 atgtcgacta tgagttgtta tgccgggagc gcgataactt ccgcatcctg gttgccatta
    67981 ccaacgcggt gctttcccgc ctggatatgg acgaactggt cagcgaagtc gccaaagaaa
    68041 tccattacta tttcgacatt gacgatatca gcattgtctt acgcagccac cgtaaaaaca
    68101 aactcaacat ctactccact cactatcttg ataaacaaca tcccgcccac gaacagagcg
    68161 aagtcgatga agccggaacc ctcaccgaac gcgtgttcaa aagtaaagag atgctgctga
    68221 tcaatctcca cgagcgggac gatttagccc cctatgaacg catgttgttc gacacctggg
    68281 gcaaccagat tcaaaccttg tgcctgttac cgctgatgtc tggcgacacc atgctgggcg
    68341 tgctgaaact ggcgcaatgc gaagagaaag tgtttaccac taccaatctg aatttgctgc
    68401 gccagattgc cgaacgtgtg gcaatcgctg tcgataacgc cctcgcctat caggaaatcc
    68461 atcgtctgaa agaacggctg gttgatgaaa acctcgccct gaccgagcag ctcaacaatg
    68521 ttgatagtga atttggcgag attattggcc gcagcgaagc catgtacagc gtgcttaaac
    68581 aagttgaaat ggtggcgcaa agtgacagta ccgtgctgat cctcggtgaa actggcacgg
    68641 gtaaagagct gattgcccgc gcgatccata atctcagtgg gcgtaataat cgccgcatgg
    68701 tcaaaatgaa ctgcgcggcg atgcctgccg gattgctgga aagcgatctg tttggtcatg
    68761 agcgtggggc ttttaccggt gccagcgccc agcgcattgg tcgttttgaa ctggcggata
    68821 aaagctctct gttcctcgac gaagtgggcg atatgccact ggagttacag ccgaagttgc
    68881 tgcgtgtatt gcaggaacag gagtttgaac gtctcggcag caacaaaatc attcagacgg
    68941 acgtacgttt aattgccgcg actaaccgcg atctgaaaaa aatggtcgcc gaccgtgagt
    69001 tccgtagcga tctctattac cgcctgaacg tattcccgat tcacctgccg ccactacgcg
    69061 agcgtccgga agatattccg ctgctggcga aagcctttac cttcaaaatt gcccgtcgtc
    69121 tggggcgcaa tatcgacagc attcctgccg agacgctgcg caccttgagc aacatggagt
    69181 ggccgggtaa cgtacgcgaa ctggaaaacg tcattgagcg cgcagtattg ctaacacgcg
    69241 gcaacgtgct gcagctgtca ttgccagata ttgctttacc ggagcctgaa acgccgcctg
    69301 ccgcaacggt tgtcgcccag gagggcgaag atgaatatca gttgattgtg cgcgtgctga
    69361 aagaaaccaa cggcgtggtt gccgggccta aaggcgctgc gcaacgtctg gggctgaaac
    69421 gcacgaccct gctgtcacgg atgaagcggc ttggaattga taaatcggca ttgatttaac
    69481 tgcaaattgc cggacagatc tgcctgtccg gcatactatt cacgagtttt tttcggacga
    69541 tatttttccg gcagttctgg caccggacgc ttgtcatcga tgagatgacg cacggttaag
    69601 atcggatgac gccacagcat tcgcggcccg gcccaacgca taatctgttt catctcttca
    69661 cgctttgctg gctggtaaca gtgcaccgga cactgcttac aggctggttt ctcttcgccg
    69721 aacacacatt tatccagccg cttttgcgcg taaacaaaca acgcctcgta atgctccggc
    69781 tccgctgacg cctgcgggca tttcgcttga taaagatcga tcattttttt aatcgtcagt
    69841 ttttcacgag agatacgctt gccggacatg ctgcctccac ctcattaaga tgtatttata
    69901 ttacatctta atcttaaagg gcactatgac tctaaagaag aaaggttcgc caaccgacgc
    69961 aattttgcgt acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg
    70021 aaccagcatc aagaactcac cgttcgcttc ttcccctgaa atgattaact ccggtatcat
    70081 gtgcgcctta tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga
    70141 accggacata accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag
    70201 cagtatctca ggctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat
    70261 ttttatgaac tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg
    70321 accaaacgcg gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg
    70381 gtggaaaact atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa
    70441 attggcgatc cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg
    70501 ccaggcacca tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct
    70561 atctggcagg acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt
    70621 cgcctgagcg aaccggccga ccgcgaaacg atggcggcag agctgcaacg cactaatccg
    70681 gcggaactgc tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc
    70741 ctgcgccgtc gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg
    70801 caatttggca ctcgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt
    70861 gctgccggtt gtctgttgca gtatgcgaaa gatacccaac gtacgactct gccgcatatt
    70921 cgttccatca ccatggaacg tgagcaggac agcatcatta tggatgccgc gacgcgtcgt
    70981 aatctggaaa tcacccagaa cctggcgggt ggtgcggaaa atacgctggc ttctgtgctc
    71041 gactgcaccg tcacgccgat gggcagccgt atgctgaaac gctggctgca tatgccagtg
    71101 cgcgataccc gcgtgttgct tgagcgccag caaactatcg gcgcattgca ggatttcacc
    71161 gccgagttgc agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg
    71221 gctttacgaa ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg
    71281 ccggagctgc gtgcacagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag
    71341 aagatgggcg agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg
    71401 ccagtgctgg tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag
    71461 tggcgcgcgc tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt
    71521 gaacgtaccg gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt
    71581 caaatcagcc gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg
    71641 aaaaacgccg agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc
    71701 tcaaaaggca aagcactggc tctggaaaaa cagctttatg aagaactgtt cgacctgctg
    71761 ttgccgcatc tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg
    71821 gtgaacctgg cggaacgggc ctataccctg aactacacct gcccgacctt tattgataaa
    71881 ccgggcattc gcattaccga aggccgccat ccggtggttg aacaggtgct gaatgagccg
    71941 tttatcgcca acccgctgaa tctgtcgccg cagcgtcgca tgttgatcat caccggtccg
    72001 aacatgggcg gtaaaagtac ctatatgcgc cagaccgcgt tgattgcgct aatggcctat
    72061 atcggcagct acgtaccggc gcaaaaagtc gagattggcc cgattgaccg tatctttacc
    72121 cgcgtaggcg cggcagatga cctggcttcc gggcgttcga cctttatggt ggagatgacc
    72181 gaaaccgcca atattttaca taatgccacc gaatatagtc tggtattgat ggatgagatc
    72241 gggcgtggaa cgtccactta cgatggtctg tcgctggcgt gggcgtgcgc ggaaaatctg
    72301 gcgaataaga ttaaggcatt gacgctgttt gccacccact atttcgagct gacacagtta
    72361 ccggagaaaa tggaaggcgt cgccaacgtg catctcgatg cactggagca cggcgacacc
    72421 attgccttta tgcacagcgt gcaggatggc gcggcgagca aaagctacgg cctggcggtt
    72481 gcagctctgg ccggcgtgcc aaaagaggtt attaagcgcg cacggcaaaa gctgcgtgag
    72541 ctggaaagca tttcgccgaa cgccgccgct acgcaagtag atggtacgca aatgtctttg
    72601 ctgtccgtac cagaagaaac ttcgcctgcg gtcgaggcac tggaaaacct ggacccggat
    72661 tcactgactc cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa
    72721 aaattcccga tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa
    72781 ataccctgta taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctat
    72841 caaaaaatca atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa
    72901 tatcagttat tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt
    72961 atttctgtcg gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta
    73021 aaccagccct ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc
    73081 gcgaccggcg atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat
    73141 gattcagagc aaaaagaagc tacagatctg ttgctgaaat tccatcacct tccacatatt
    73201 attgaaatca ctaacgacaa cataaaatat gtcatcgcac atgcagatta tccggggagt
    73261 gaatatctct ttggtaaaga aatagcggag agcgaattac tctggcctgt tgatcgtgtg
    73321 cagaaatcgc ttaatggcga gttacaacaa ataaacggcg ctgattattt tatatttgga
    73381 catatgatgt ttgataacat tcagacgttc gctaaccaga tttatattga taccggatcg
    73441 ccgaaaagtg ggcggctgtc attttataaa ataaggtagt ctcatgcttc ttctgtgaag
    73501 catgagtaac cagatgttat tgcaggccat tattcatttt tcgctaccag caaagagaga
    73561 tcctgcttcg ccagcgcgcg actggcactc tccggcaaac cgtcgtctgt aataatctga
    73621 tcaaactcgc ttaatggtaa cgccagccat gtcgccacct gaccatattt cgtcgcatca
    73681 cagaccaaaa ctcgctggcg gctggcactg gcaatcgccc gtttcaccgt gactttatct
    73741 tccgctggcg tagaaatccc ccgcacactc catgacgatg cagaaataaa agcctgatca
    73801 atcatcaggc tgcgcagcat ggtcgcagcg gcttccccga cacaggaacg gttttcccga
    73861 cacactgcac cgccagtgtg aataattgtg caattactgt tgtcgagcaa gtagttcgca
    73921 ataacgaaat cgtttgtgac cacagtcagt gactccatgt gaatcagatg ctgtgctatc
    73981 gctaacgtgg tcgttcccgc atccagatag atacaacttc ccggctgaac aagacttgcc
    74041 gccagcttgc caatagccgc tttttgcgtc attgccagcg cagtttttac ctgatgagaa
    74101 ggttcatgcg ccacgcgtcc cggagactgg acgcctccgg acaccagcac aacggctccc
    74161 tgctgctcca gtttttgtaa atcccgacga atggtcatat gtgacacatt cattctgtcc
    74221 gttagttcag caatactgac aatgcctttt tcagctacca tctcaaggat gatttggcga
    74281 cgctctacgg gtatcaactt ttgctccttc ctttgtcctg ctgacattct acgctatttg
    74341 cctgcgaaac gtgcgcggcg caactaacgc ttagttcaca taaaataaca cacaatgtta
    74401 atttatgtga atcagctcac catatcgtta tcttccagcg cttatattca caatatcaaa
    74461 caaaatatca cttaaattaa caaggagagc agatgaaaac gggatctgag tttcatgtcg
    74521 gtatcgttgg cttagggtca atgggaatgg gagcagcact gtcatgtgtc cgcgcaggtc
    74581 tttctacctg gggcgcagac ctgaacagca atgcctgcgc tacgttgaaa gaggcaggtg
    74641 cttgcggggt ttctgataac gccgcgacgt ttgccgaaaa actggacgct ctgctggtgc
    74701 tggtggtcaa tgcggcccag gttaaacagg tgctgtttgg tgaaacaggc gttgcacaac
    74761 atctgaaacc cggtacggca gtaatggttt cttccactat cgctagtgct gatgcgcaag
    74821 aaattgctac cgctctggct ggattcgatc tggaaatgct ggatgcgcca gtttctggtg
    74881 gtgcagtaaa agccgctaac ggtgaaatga ctgtcatggc ctccggtagc gatattgcct
    74941 ttgaacgact ggcacccgtg ctggaagccg ttgccggaaa agtttatcgc ataggtgcag
    75001 aaccgggact aggttcgacc gtaaaaatta ttcaccagtt gttagcgggc gtacatattg
    75061 ctgccggagc cgaagcgatg gcacttgcag cccgtgcggg gatcccgctg gatgtgatgt
    75121 atgacgtcgt gaccaatgcc gccggaaatt cctggatgtt cgaaaaccgg atgcgtcatg
    75181 tggtggatgg cgattacacc ccgcattcag ccgtcgatat ttttgttaag gatcttggtc
    75241 tggttgccga tacagccaaa gccctgcact tcccgctgcc attggcctca acagcattga
    75301 atatgttcac cagcgccagt aacgcaggtt acgggaaaga agacgatagc gcagttatca
    75361 agattttctc tggcatcact ctaccgggag cgaaatcatg atcaagattg gcgttatcgc
    75421 cgatgatttt accggcgcga cggatatcgc cagttttctg gtggaaaacg gtctaccaac
    75481 ggtacaaatt aacggtgttc caacaggtaa aatgccggaa gcaatcgacg cactggtgat
    75541 cagcctgaaa acgcgctcct gtccggtggt tgaagccaca cagcaatcgc tggcggctct
    75601 gagctggttg caacagcaag gttgcaaaca gatctatttc aaatactgct ctactttcga
    75661 cagtacggcg aaaggtaata tcggcccggt taccgatgca ttaatggatg ctctcgacac
    75721 gccgtttacg gtcttctctc cggccctgcc ggtcaacgga cgtacggttt atcaggggta
    75781 tttgttcgta atgaatcaac tgctggccga atccgggatg cgccatcacc cggtaaatcc
    75841 catgaccgac agctatcttc cccgtctggt tgaagcgcaa tccacagggc gctgcggcgt
    75901 cgtttcggca catgttttcg aacaaggtgt ggatgccgtt cgtcaagagc tggctcgctt
    75961 acagcaagag ggctaccgct acgcggtgct tgatgcgctg accgaacacc atctggaaat
    76021 tcagggagaa gccttgcgcg atgccccact ggtaacgggc ggttctggtc tggcgattgg
    76081 cctggcccgg cagtggacgc aagaaaacgg taaccaggct cgcaaagcag ggcatccgct
    76141 cgctgggcgc ggcgtagtgc tctccggttc atgctctcaa atgaccaacc gccaggtagc
    76201 acattaccgt caaattgcac cagcccgtga agttgatgtg gcacgctgcc tctcaactga
    76261 aactctggct acttatgcac acgaactggc agagtgggtt ctgggccagg aaagtgtact
    76321 tgctccactg gtttttgcca ccgccagcac tgacgcattg gcagcaattc aacagcaata
    76381 cggtgcacaa aaagccagtc aggcagtaga aacactgttt tctcaactag cggcgcggtt
    76441 agcggcggaa ggcgtggcac gctttattgt cgcaggcggt gagacctccg gcgtagtcac
    76501 acagagcctg ggcataaaag ggtttcatat tggcccaacc atttcccccg gcgtgccgtg
    76561 ggtaaacgca ctggataagc ctgtctcact cgcccttaaa tctggcaact tcggtgatga
    76621 agcctttttt tcacgagccc aaagagagtt tttatcatga gcgatttcgc aaaagtagag
    76681 cagtctttgc gagaggagat gacgcggatt gccagttcat tctttcagcg cggctatgca
    76741 accggttcgg ctggcaatct gtcgctgctt ttacctgacg ggaatttact ggcgacaccg
    76801 acaggttcat gcctgggcaa tctcgatccg cagcggcttt ccaaagtcgc cgcggatggc
    76861 gaatggttaa gtggtgacaa accctcgaaa gaggtgctct ttcatctggc gctgtatcgc
    76921 aacaatccgc gctgtaaagc ggtggtgcat ttgcacagca catggtcgac ggcgctttcc
    76981 tgcctgcaag ggctggacag cagcaacgtt attcgtccgt tcacaccata cgtggtgatg
    77041 cggatgggaa atgtcccgct ggtgccttat taccgaccgg gcgataaacg catcgcacag
    77101 gatctggcgg aactggcagc agacaatcag gcttttttac tggcaaatca tggcccagtg
    77161 gtttgcggtg aaagcctgca agaagccgcc aacaatatgg aagagctgga ggaaacggca
    77221 aagctgattt ttattctcgg tgaccgcccg atccgttatc tgaccgcagg tgaaattgcg
    77281 gaattaagga gttaatgcaa tgcctcgttt tgcagctaat ttatccatga tgttcaccga
    77341 agtgcctttt attgaacgct tcgccgcagc gcgaaaagcc gggttcgatg ctgtggaatt
    77401 tctgtttccc tatgactact ccaccctgca aatccaaaag caactggagc aaaaccatct
    77461 gacactggcg ctgttcaata ccgctcccgg agatattaat gcgggggaat ggggattatc
    77521 cgcccttccc ggacgtgagc acgaagcaca cgcagacatc gacctggcac tggaatatgc
    77581 attggcgctc aattgtgaac aagtccatgt gatggcaggc gtcgtgcccg ctggcgaaga
    77641 cgctgaacgg taccgggcag tatttatcga taatctccgc tacgctgccg accgctttgc
    77701 gccacacggc aagcgaatat tagttgaagc actcagcccc ggcgttaagc ctcattatct
    77761 cttctccagc cagtatcagg cactggctat tgttgaagag gttgcgcgag ataacgtgtt
    77821 tattcaacta gatacttttc atgcgcaaaa agtagatggc aacctgacgc atttaatccg
    77881 cgactacgct gggaaatatg cgcacgtaca aattgccgga ctacctgatc gacatgaacc
    77941 ggacgatgga gaaatcaact atccgtggct gttccgcctg ttcgatgagg tgggatatca
    78001 gggctggatc ggttgtgaat ataaacctcg tggcctcacc gaagaagggc ttggctggtt
    78061 tgacgcctgg cgctaattcg ttaaatccct aattacaacg tacccataca tccccccatc
    78121 tacggatggg gtaggttaac tataactttc agacagggtt tcccatgtcc acaattacat
    78181 tgttatgcat tgcgttagct ggcgtaatca tgctgttgct gctggtcatc aaggcaaagg
    78241 tacaaccatt cgttgctctg ctcctcgtca gcctgttagt cgcacttgcg gcgggtatac
    78301 cggcgggcga agtgggtaaa gtgatgatcg ccgggatggg cggtgtgctt ggttccgtca
    78361 ctattattat tggtctgggc gctatgctgg ggcgtatgat cgaacactct ggtggtgcag
    78421 agtcactggc taattatttc agtcgcaagt taggtgacaa acgaactatc gctgcgctga
    78481 ctctggcagc gttcttcctc ggtattcccg tcttctttga tgtcggcttt attattcttg
    78541 cgccaatcat ttacggtttt gccaaggttg ccaaaatatc gccactcaaa tttggcctgc
    78601 ctgtcgctgg aatcatgcta actgttcatg tggcggtacc gccgcatcca ggccctgtcg
    78661 ccgcagcggg gttactccac gcagacatcg gctggctaac catcatcggt attgcgattt
    78721 ctattcccgt agggattgtt ggctactttg cagcgaaaat aatcaatacg cgccaatatg
    78781 cgatgtcggt ggaagtactg gaacagatgc aactggctcc ggccagtgag gaaggcgcaa
    78841 caaaattaag cgataaaata aatccaccgg gcgtcgcgct ggtcacctcg ctaattgtta
    78901 ttcctatcgc gattatcatg gcgggtacgg tttccgcaac actgatgccg ccttcgcatc
    78961 ccctgcttgg tacgctacag ctgatcggct caccaatggt agctctaatg attgcgctgg
    79021 tgctggcatt ctggttattg gctttacgtc gcggctggag cttacaacac accagcgaca
    79081 ttatgggctc agcccttccc actgcggcgg tagtgatttt ggttactggt gctggagggg
    79141 tatttggcaa agtgctggtg gaatcgggcg tcggcaaagc ccttgccaat atgctacaaa
    79201 tgattgacct gcctctgtta ccagccgcat ttattatttc actggctctg cgtgcatcgc
    79261 aggggtcagc aaccgtagca atcctgacaa ccggcgggtt actctcagaa gcggtgatgg
    79321 gattgaatcc gattcagtgc gtattggtga cgctggcagc ctgctttggt gggcttggcg
    79381 cgtcacatat taatgactca gggttctgga ttgtgaccaa atatctgggg ttgtcggtag
    79441 cagacggtct gaaaacctgg acagtgttaa cgaccattct cggttttacc ggattcttaa
    79501 ttacctggtg cgtatgggcg gtaatttgac cttaataaaa aggtccgatg ggcatcggac
    79561 cttttattta acgcttacct tccaccagca gcggcgggat gctcggcacc attggcgcgt
    79621 catcaatatc tttctgcgtc atgcggaacg cttcgggata atgttcgcgg ctggtacggc
    79681 gcagcggttc ggtatcgcgc agcggttcgg tatcgcgcca ggtataaagg caatgctggc
    79741 actgatatac cgtccagaca tctttcaccg gcgatttcgc catcacttca atctgttcat
    79801 cggcacaacg tggacaaatc atcttcttct ccttatttac gtgcagccag catagcggtc
    79861 agtttttcag cccaggcttt ggtttccggt aaatccacca ccggttggct gtagtgacca
    79921 cggttgtccg gggcgacagg cgtagtggcg tcgataatca gcttgtcggt gatccccgca
    79981 gggcttgagc ctggatcgag ttccagcacg gacatattcg gcaactgcac caaatcccct
    80041 gccgggttca ctttcgagga gagcgcccac atcacctgcg gcaggttgaa cgggtcaacg
    80101 tcttcatcga ccataatcac catcttcacg tagcccagac catgcggcgt ggtcattgcg
    80161 cgcaggccca ccgcgcgggc aaagccgccg tagcgttttt tggtggagat aatcgccagc
    80221 aggccatggg tgtacatggc gtttaccgcc tgcacttcag ggaactcggc tttcagctgc
    80281 tgatacagcg gcacgcaggt ggctggcccc atcaggtagt cgatttcggt ccacggcata
    80341 ccgaggtaca gcgattcgaa aatcggcctg gtgcggtaag agactttatc gatgcgcacc
    80401 acggtcatgt tacgcccgcc ggagtagtgc ccggtaaact caccgaacgg cccttcgatt
    80461 tcgcgtttac ggctttcgat gaccccttcg aggatcactt ctgaacccca cggcacatca
    80521 aaaccggtca acggggcggt ggcgatcggg tacgggcttt cacgcagcgc gcctgccatt
    80581 tcgtactcgg actgatcata tttcagcggc gtggccccca tcagcgtgat gatcggatcg
    80641 ttaccgagcg tgatcgcaat cggcagatct tcaccgcgct cttctgcttt atgcagatgc
    80701 agggcgatat cgtgcatcgg caccggttgc aggccgagct tacgcttgcc cttcacttcc
    80761 atgcggtaga tgccgacgtt ctgcttgccg aagttatccg ggtcgagcgg atcgcgggaa
    80821 accacgcacg ctttgtcgag atagaaaccg ccatcgccat cgtttaaacg aaacagcggc
    80881 aggatatcga acaggttaat ctcttcgcca tcgacggtgt tctgcgccca ggctggattg
    80941 gcgcggcgct ccggggcaat cgggaagtta tcccagcggc ggataaactc atcaatctgc
    81001 tttttgaccg gggtgtttgg tggcaggcca agggaaatcg cgtggttctg ccaggaaccg
    81061 atggtattca tcgtcacgcg ggcatcggta aagccgcgaa tattatcaaa ccacagcgcg
    81121 ggcgcgccgt cgccgatacg cccggtggcg ttagctgctg ctgccagatc cggctcagcg
    81181 ttcacctctt cgctgatttt cagtaactgg ccgtggtcat caagcgcctg taaaaagctg
    81241 cgtaaatcat caaatgccat tattcattct cctgagaaaa attccgggcc tgcggcaatc
    81301 cttgccagcg cctggcgtgg ggatgttcga ggccaaattg atccagcacg cgggctacca
    81361 catggtggac aatgtcatct accgtttcgg gatggttata aaaggcaggc atcggcggca
    81421 ccatcgccac gcccatgcgt gaaagtgcga gcatattttc gagatggatg gtgctaagcg
    81481 gcatttctcg cggcaccagc accagtttgc ggccttcttt gagcacgacg tccgccgcgc
    81541 gccctaccag gccatcggcg taaccagcgc ggataccggc gagcgttttc atactgcacg
    81601 gaataacgat catgccgtcg gtacgaaaag aaccggatga gatgatcgcc gcctgatccg
    81661 ccgggttatg gctgaagtct gcgagggcag caacatcgcg agcgctgtaa ggcgtttcca
    81721 gttcaatggt ggttttcgcc cacttcgaca tcaccagatg agtctcgaca ttcggcatct
    81781 cccgcagcgc ttgcagtaat gccacaccaa gaggcgcacc ggtagcccct gtcatcccga
    81841 cgatcagttt cattgcttat cctcttaatt gttcgtatac gaacgttatt attaacatac
    81901 tccttcattt ccacagcggc aaggattgaa aagtaacaac atcttcttcg tcactaaaaa
    81961 accgctatga tagcaacaga gaattagccc ggagtttaca tggcgttacg aaataaagcg
    82021 ttccatcagt tacgacagct ttttcagcag cacacggctc gctggcagca cgagttacct
    82081 gacctgacca aaccacagta cgcggtgatg cgcgccattg ctgataaacc tggtatcgaa
    82141 caggtggcac tgatagaagc agctgtcagc accaaagcaa cgttagcaga aatgttggca
    82201 agaatggaaa atcgtggctt agtcagacgt gaacatgacc ccgctgataa gcggcgacgc
    82261 tttgtctggt tgactgccga aggcgaaaag atacttgctg cggcgatacc cattggtgac
    82321 agcgtggatg aggaattttt ggggcgtttg agtggagaag agcaggagct gtttatgcag
    82381 ctggtgcgca agatgatgaa cgcatagggg gcaaatttcc ggatgcgacg ctatcgcgtc
    82441 ttatccagac tacaaataca ccgactcacc aaccgaaaga aaaaaggcca gcctcgcttg
    82501 agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga tattcagccc
    82561 ctgcgtttgc aggatttcgc gcaaacggcg caggccttca acctgaatct ggcgaacacg
    82621 ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt cgtaccccag
    82681 caaaccgaat cgacgtgcca gcacttcacg ctgtttggcg ttcagctcga acagccattt
    82741 gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt tctctttttc
    82801 atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg tgtctaccga
    82861 ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct tatccagttg
    82921 ctctgcgatc tcttccgcac ttggttcatg gtccagctta tgggacaact cacgtgcggt
    82981 tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg
    83041 gttcataatc gcccgttcaa tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg
    83101 gaaaccacgt tccgggtcaa acttctctac cgcgcggatc agccccaggt tgccctcttc
    83161 gataaggtcc agcaacgcca gaccacgatt gccataacgg cgggcaattt ttaccaccag
    83221 acgcaagtta ctctcgatca tccggcggcg agaggcgaca tctccacgca gtgcgcgacg
    83281 cgcaaaataa acttcttctt cggccgttaa cagtggtgaa taaccaatct caccaaggta
    83341 aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc gataacagtt cctcttcggc
    83401 caaatcgtta tcactgggtt cctcttctac taaggccttt tcgtcaaaaa cctcaactcc
    83461 gttctcatca aattccgcat cttcatttaa atcatgaact ttcagcgtat tctgactcat
    83521 aaggtggctc ctacccgtga tcccttgacg gaacattcaa gcaaaagcat ggttccgccg
    83581 atttatcgct gcggcaaata acgcagcggg tttacggatt tccccttgta acgaatttca
    83641 aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc
    83701 ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa
    83761 tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accagcataa
    83821 acaacgcggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc
    83881 cccttgttgc ccccctcaga agcgccaaag gtttcgatca ttttgccctc agtcggccag
    83941 cgccaggtgg agataggcgt actggttgat gtactgctga cagttggctc ggttgtgctt
    84001 gctgttggta ccgttacagg cgctgtgacc gtggtcgcag ctggcttgtt gttcggcaac
    84061 attttgttag cactctgttc acccgaagat tcagaatacg taattgtcgg ttgcgacgca
    84121 acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc
    84181 tgggtaatgg catttccgcc agtgattggc gtaccggaag cattacccac ctgcaaggtc
    84241 tgaccaacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa
    84301 tcgttgccag taatccaggc gatatagaaa agtgtgtcgc cttttttcac ggtataggta
    84361 ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac gatgcgtccg
    84421 ttttccatct gtaccggctg ctgagctact ggctgcactg gctggatttg cggttgttga
    84481 gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg gttgctgggc tgtagacgtc
    84541 gtccccattt tcggcggcgg cgtaatcaac ataccagaat tggtatttgc aggcgcattg
    84601 ccattaacgg agctgaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat
    84661 agcgaaacca gtgacaaagc cgcaatgcgg cgaacggtga attttgggct tcccgcgctc
    84721 atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcaccc
    84781 tactgaacac tggaaaagat gttcacgata cgctgacctg cggcaaaata accaggaaaa
    84841 atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac
    84901 ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg
    84961 gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagcgc
    85021 agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg cgcgtgcctg
    85081 ccaaccttgc catccatcgc catgacgggt tgaaacatta tgtaaatcaa gatttttcag
    85141 gcggcgacgc gcctgccact gcaaaccttt aatccgttcg accgagcaaa catgctggac
    85201 aagatgcgcc aggattgccg tttgatatcc cgaaccagtg ccaatttcca gcacccgcga
    85261 ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaattgt
    85321 ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc
    85381 aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc
    85441 ttgcgcacgt aattgatcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact
    85501 cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca
    85561 tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt ccccggaccg
    85621 gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc
    85681 tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg
    85741 atttgatcca agggtaaatc cggaacgtta atattaagaa tacgcccggt gcgcaacggc
    85801 tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc gtaatgttta
    85861 tgcccgtcaa gcgagacggc aagcgccgga aaacctaaat gacggccttc catcgcggcg
    85921 gctaccgtac cggaataaat aacgtcatcc cccagattcg gcccggcgtt aattccggac
    85981 acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac gcaatcggtc
    86041 ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg cagggaggat
    86101 tccagtgtca gagaatttga agcgccgctg cggttacgat cgggggcgac cacctgaacg
    86161 tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca
    86221 tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct
    86281 ggttgcaaaa ctacccgccg gaagccagaa acggatctct acggtgacgt catcccacca
    86341 attccagctt aattgttgcg gatacagcaa catcgctctg cgcgcggctt caactttttc
    86401 gcgcaccagt aaagcttgta attcagtttc tgcggcgaca gctgcctttt cgaatgccag
    86461 cgcttcacgc tgagttcccc attcgccact gcccggcaat gcggcggtta tcatcagctc
    86521 tttatcgttg acgcgacgct gtaattccgc cagttcttcg gtggttgcta caaaccagct
    86581 accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt
    86641 gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca accaaaaact
    86701 ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgcagcg cgccctgcaa
    86761 gttgctaccg ccaatcccaa aacgttgggc accgaagtag ttcggtacac cttttacgca
    86821 aatatcgatc agacgttgtt caacgtcatc gcgattgctc acttcgcgca gaaccagggt
    86881 aaaggcgtta cctttcagcg cgcctaaacg cagcttgcgc ttgtgccgcg catactccag
    86941 cacctggcag ccttccagtt gaaaggcgct cagatcgggc atttccttgc ctggcacgcg
    87001 agcgcataac cactgttccg taacagcatg tttgtctttt tgcccagcga agctgacttc
    87061 acgggcatga attttcagga atttcgccag tgcatccgcc acaaaacggg tattgcagcc
    87121 gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc
    87181 ttccaccacc acaaagtctt ccggattggc tttcagcagc ccggtgcctt gcggtttacc
    87241 gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc
    87301 gcttcacagg caatcccttc cccacgtccg gtaaatccaa gtttttccgt agtagtggct
    87361 ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt
    87421 ggaatgtgcg gcaacatctt cggtgcctga gcgatgatag tgacatcgac gttgcccagg
    87481 gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg gctgtcggca
    87541 cctttaaatg ccggatcggt atccgggaac agcttgccga tatcccccag cgccgccgcg
    87601 ccaagcaatg catcggtcaa cgcatggagc gccacgtcgc catcagaatg cgccagcaat
    87661 cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggca
    87721 tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga
    87781 ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta tccgcacggc
    87841 cttcgaccaa ctgaggatgg aatccgcaat attccagcgc cgaggcttcg tcggtaatag
    87901 tcgcgccttc atttagagcg cgcgtcagac agtcatgtaa cagctcacga gggaaaaatt
    87961 gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaatg gcatttttgc
    88021 ccggttcggc acgtttcatc gtatcgcgca ctggtgcggc taggatccct cccgtgcggc
    88081 tggtttcgct caacgccaac aatcgcgcga ggtcatcctg atgcagacaa ggacgagcgg
    88141 cgtcatgcac caatacccac tgcgcgtcgc cagcggcttt caaacctgcc agcacggaat
    88201 cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc agaggaagtt
    88261 gtgcaaaacg gctatcgcca ggacttatgg caatgacgac acgtttcacc cggggatgcg
    88321 ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt accgattgag agatattgct
    88381 taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg gcgcaaacat
    88441 ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt tgccccgcag
    88501 actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc ggcctggtca
    88561 tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa
    88621 tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg
    88681 ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac cacagcgaat
    88741 actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg
    88801 aaaaacggca tcatcatccc atgcatccga agacgactct acatcctctg ttggggatac
    88861 cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca
    88921 gaacataatc tcattattag ttacggtttg aattatgaac agaggagacg ggaaagtaca
    88981 aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt caccagggtg
    89041 aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat ccccaccact
    89101 accgcgacgg gcatcagcgc cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg
    89161 ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat aaaaccgacc
    89221 acggcaccag gcagtgacca tgtggtttct tcatcctcag taaggctgtc gttatttgtt
    89281 agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa ccgggcatgg
    89341 aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg
    89401 ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg attcaggcgc
    89461 ttcgtaaaca gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata
    89521 taagcctttt ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa acacttcgat
    89581 aaagcgccct tctcctacgc gttcgcgaac catctggcgt tcggcgcggt gtggcgagat
    89641 aaatgcggtc agcactacca gtccggcttc aaccatcaaa ttcgccactt caccgacgcg
    89701 acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata atccgtggcg
    89761 aacattatcg ccatccagca gatacgtact gacgccgagt ttatgtaacg cctcctccag
    89821 caccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca gcactacacc
    89881 acgatgaccg tggtgtagct cgcgttgttg cacagtgacc ggatggctat gccagacgac
    89941 gttttcgtca tgcagcgcca ttatttatcc cccagcaaat cgcgcgcgcc ccagtgcgga
    90001 aagtggcgac gaaccagagc attcaattcc agttcgaatg cactgaattc agatggcgca
    90061 gcagttgcct ggctaactgg ctcgtgcacc ataccggcac ccacggtcac attgctcagg
    90121 cgatcgataa aaatcagccc acccgtcacc ggattttgtt gataacgatc taacaccagc
    90181 ggctcgtcaa aagtgagatc cacgaggccg atcccattca gtggcaggtt ttcaacttca
    90241 cgctgggtaa ggttattaat atcaacctga tagcgaatgc catcaacacg cgcgcgcgtc
    90301 ttcttaccgg caattttgat gtcgtaactc tgccctggag aaagcggctg ttccgccatc
    90361 cataccacat ccaccgacgc gctctgcacc gccggtaacg cttcgtctgc cgccagcagc
    90421 agatcgccac ggctgatgtc gatctcatcc gtcagcacca gggtgatcgc ttctccggca
    90481 aaggcttctt cgcgatcacc atcaaaagtc acgatccgcg cgacgtttga ttccacacca
    90541 gaaggcagca cttttacacg ttgcccgact tccacgcgac cggatgccag cgttccggcg
    90601 taaccacgaa aatcgagatt cgggcgatta acgtactgca ccgggaagcg cattggctga
    90661 gcatccacca ctcgctggat ctccacggtt tccagcactt cgagcagtgt cggaccgctg
    90721 taccacggca tactttcact ttgcgaagcc acgttgtcgc cttccagcgc agagagcggc
    90781 acaaagcgaa tatccagatt gccaggtaac tgttcggcga aagtcaggta gtctttacga
    90841 atgcgggtga atgtctcttc actgtaatcc accagatcca ttttgttgat cgctacgacc
    90901 agatgtttga tccccagcag cgtggagata aaactgtgac gacgggtttg atcgagcacg
    90961 cctttacggg catcgatcag taagatcgcc agctcacacg ttgaggcgcc ggtcgccata
    91021 ttgcgggtat actgctcgtg cccgggggtg tcggcgataa taaatttacg cttctcggta
    91081 gagaaatagc ggtaggccac atcaatagtg atgccctgtt cgcgctcagc ttgcaggccg
    91141 tccaccagca gagccagatc cagtttttcg ccctgggtgc cgtgacgctt actgtcgtta
    91201 tgcagcgatg agagctgatc ttcgtagatt tggcgggtat cgtgcagcag gcgaccaatc
    91261 agggtacttt tgccgtcatc gacgctacca caggtcagaa aacgcagcag gcttttatgt
    91321 tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg
    91381 gtgttcatct taaaaatacc cctgacgttt tttcagctcc atagaccccg cctggtcgcg
    91441 gtcaatcacg cggccctgac gttcactggt ggtggaaacc agcatctctt cgatgatttc
    91501 cggcagtgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc ccagcgtacg
    91561 gaaacgcacc atccgttttt taatcacttc gcccggttgc aggtcgatac ggttgtcatc
    91621 aatcatcatc aacataccgt cgcgttccag aaccggacgt tccgcagcga gatatagcgg
    91681 aacaatgtcg atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga
    91741 gagcgggaag acgcggatgc tttcgccttt gttaatttgc ccgttgtagt tgtgccacag
    91801 ctccgggcgc tgatttttcg gatcccagcg atggaagcgg tcacggaaag agtaaattcg
    91861 ctctttagcg cgggatttct cttcgtcacg gcgcgcacca ccgaaggcgg catcaaaacc
    91921 gtatttgttc agcgcctgtt tcaggccttc agttttcata atatcggtat gtttcgcgct
    91981 gccgtgcacg aatggattaa tccccatcgc cacgccttcc gggtttttat gcaccagcag
    92041 ttcgcagccg taggctttag cagtacgatc gcggaactca tacatctcgc ggaatttcca
    92101 gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc
    92161 caaatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa
    92221 ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgagt
    92281 aagtcgtttt tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg
    92341 tttcgactat agggagcgta agagaacgaa tgaaattacc aattagaatg agtagttcct
    92401 taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttcgg
    92461 gcgtttagga agatttgaaa ttgttttagc gcagcggcag tttcatacta tggcggtaaa
    92521 aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg
    92581 ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct aaacctggcg
    92641 attttgctaa tacccaggca cgacatattg ctactttctt tccgggacgc atgaccggaa
    92701 ctccagcaga aatgttatct gccgattata ttcgccaaca gtttcagcaa atgggttatc
    92761 gcagtgatat tcggacattt aatagtcggt atatttatac cgcccgcgat aatcgcaaga
    92821 actggcataa cgtgacagga agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc
    92881 agatcatcat tatggcgcat ctggatactt acgccccgct gagcgatgct gacgccgatg
    92941 ccaatctcgg cgggctgacg ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca
    93001 tgctggaatt ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg
    93061 cgaccagcgg cgaagaggaa gggaaattag gcgctgagaa tttactcaag cggatgagtg
    93121 acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata
    93181 aattgtattt caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca
    93241 gggcgctggc aattgcgcgc agtcacggaa tagccgcaac gaccaatccg ggtttgaata
    93301 aaaattatcc gaaaggcact gggtgttgta atgacgcaga aatattcgac aaagcgggca
    93361 ttgctgtact ttcggtggaa gcgactaact ggaatcttgg gaataaggat ggttatcagc
    93421 aacgcgcaaa aacagccgca ttccctgcgg gaaatagctg gcatgacgta agactggata
    93481 atcaacagca tattgataag gcacttcctg gaagaataga acgtcgctgc cgtgatgtta
    93541 tgcggataat gctaccgctg gtgaaggagc tggcgaaggc gtcttgatgg gtttgaaaat
    93601 gggagctggg agttctaccg caggggcggg gaattctaag tgatatccat catcgcatcc
    93661 agtgcgcccg gtttatcccc gctgacgcgg ggaacacaaa accaaacttc tccataaatt
    93721 ccatagccgc ggtttatccc cgctggcgcg gggaacacga gtctatcagc gacactaccg
    93781 gcaatagcga cggtttatcc ccgctggcgc ggggaacacg gctttaaaat tgtgttcccg
    93841 tctggcgctg ggggtttatc cccgctggcg cggggaacac gcatccatgc cgacgccttt
    93901 acgtgtgcgg ggcggtttat ccccgctggc gcggggaaca cgcgcgaatt tgtgcgcatg
    93961 gggcgcattt ttggcggttt atccccgctg gcgcggggaa cacacgatgg cgatgcgtga
    94021 gaaagggggt cgatacggtt tatccccgct ggcgcgggga acacagttcg ctgagtagcc
    94081 ttttttctgt gcctaacggt ttatccccgc tggcgcgggg aacacactaa gcatacataa
    94141 ctgtttttaa acaaatttat tccgcatcaa caatctacca actaaattaa aacatttcct
    94201 tatttttaaa gaacggataa cttattgatt atcaacagga agaaaagaaa ccaaacgtaa
    94261 cccatccaaa tccaccggaa tacgtctgtt ttctccccag gtctgaaatt caaaacccga
    94321 ctcggtattg gtcgcccagg ccatcaccac atttccgcaa ccagccagtt gggtaatttg
    94381 ctgccagatc atctcccgaa tacgttttga tgtatcacca acatacacac cggcacgcac
    94441 ttccagtagc cagattgcga gccgtccacg taagcgcggc gggacatttt ctgtaacaac
    94501 cacgaccatg ctcatcagcc gcgtcctcga tgaccgctat cgcccaacgt ttcaggttcg
    94561 gggatggctg gcggtaacat atccggcgca ggttgtggcg gttcaatttc acctgcggca
    94621 aggacttcct caattaacgg tattaatttg cccgttaact tagtgctacg gaaaatatcg
    94681 cgacaggcta atctgacttc tttatcaggt tctgcgggtt gcctcgctgc tatttcaaat
    94741 gcctttggca caaccgaatc aaatttaatg atatcggcta tgtcataaac aaatgaaagc
    94801 ggtttgccac tatggataaa cccaatagcg ggcgcatatc ccgcggctaa tactgccgct
    94861 tcagatatac cgtacagaca tgatgtagca gcactgatgc agcgattcac aacatcgcct
    94921 ttttcccagt ctttaggatc gtatttgcga ccattccatt tcacaccata ttgtttcgcc
    94981 agtaatgcat acgtctggcg aacgcgagat ccctcaattc cacgtaactg ctccactgaa
    95041 cggcgagctg gctgtggttc acgaaaacgt aattcataca ttttgcgcac caccttcaac
    95101 cgtagatctt ccgttaaagc cagctttgcc tggtagagta atttatctgc ccgcgcccca
    95161 ccgggttgtc cggaggagta aacgcgaacg cccgcttcac cgacccagac cagcagtgtt
    95221 cccaccgtgg cggccagatg caccgccgcg tgggaaactc tcgttcctgg ttcgagcata
    95281 atgcaggcga ccgatcccac cggaatatgc gttcggatcc ctgttttgtc gatcagcacg
    95341 aaagcgccgt ccagtacgtc gatctgaccg tactggagga agatcatgga ggtgcgatct
    95401 tttaatggga tcgggctcag tggtacaaac gtcacgcctc tgctccgggt ttgatcagca
    95461 tcagaccaca gccgaacgcc cggcttttac catacccctg gctcaggcgt tgcacaaata
    95521 acccaggatc ggtgaccgta agcatccccg tataatccac gctactgaac tggatcagtt
    95581 gccgggagtt ttcccgccgc agttgctgtt gtcggtaggc atcaaccgag gtatccagca
    95641 gtgtaaaacc gcttctctcc ccctgcgctg ccagccagtc cagcgccgcc tgttgttgat
    95701 gcaaccagac atcacttcct tccgcctgcc ccctcacctg ccgtttcgcc tccatcagca
    95761 gatcgtggcg cttgcccgct ttacagattg ttggattcgc ccgcaggttg aaacaaagtt
    95821 gttgtccggt acacagctcg ggcgcaaatg accggcattc tatggtgaac gtatcacttt
    95881 ccgccgggcg ttcctgcgac agtacaaaaa agcgaaacgc gccctggagt tcttcccgac
    95941 gataaagaaa ttgcctttct ttgccgccag ggaagagatc ccacagccac tgatgcatca
    96001 catattcacc gcgatccacc agatgcagca actgcgcagg cgaaagctgg ccggtatgta
    96061 aggttattct tgagaggtac atggttcctc cttgctgagc cacggcccct gattgacggt
    96121 gcgttcccca aacagccact gctgacgatt taaaggaaca tcccggcggc gtaatatttt
    96181 gctcgcgacc aggccgtcgt gtttcccttc ccaccagcat tcatcctgaa gtttcgggag
    96241 tgagactttc agtttatgga aatgatcctg atattgttgg tatgcattac ataaggcatc
    96301 acacgcgttg ccttcgagca gtaacggcgc aagaggtaat gccagcggat gactttttcg
    96361 ccccagataa agcggaaaaa ctggatggcg taaaccgtcc tgcaactgtt cgaggctgta
    96421 aggcgcatcg gctgttgttg ccaccgccac catccaccag gcatcggtgt agtagtcgcg
    96481 ccgggagata atcgcgctca aaagctcagg gtcgctcaac tcttcgcgac ggctgaaata
    96541 acgtgcttta cgcacctctt ttggcatctg gaccgtgtga taatcccgtg cccagcgcgg
    96601 gttacggctg gcgcaaacca ccagtgaata gtggcgatta aacgcgttta agcgttcggt
    96661 atcatcacgc cgaatcccta ccccggcagc cagtagcccc agcaatgctg agcgcgaagg
    96721 aagttcatgg gtatgacgaa cttcacctgg agcatcgacc ccccaggacg ccatcggccc
    96781 atgaagctga aaaatcagat attggctcat aagccgcccc ttacgcgcag ataaagtcca
    96841 gcacgtcctt catgcttccc tgtttgttca tcacgtcaaa acttgcgcat tcggtcttct
    96901 gttcgtagac cgtattcatg ttttcgcgaa gcgttgtaat acgctgcacc gccacatcta
    96961 actgacgtgt gccattaatg ggttcataga aagccgccgc cagagagcgt ggttgatcgg
    97021 tgcctttttc tgccagcgcc caggaggcgt aggcacggct ggcaaagctg ttctgtttgc
    97081 cggttgggga gactttaagt gcggcttccg taaaggcacg tatggtctga ttagctaacg
    97141 cttcgtcacc gccgaggttt tctaccagca gatctttatc aatgcagata taggtgtaga
    97201 acagcgcaga accgaatccg gtttcatcta catgaccggc accggcatct tcagaagcct
    97261 ggcgtaaatc atctacggcg gtgaaaaaat cattttcgac aatcgtttca ctgacaccaa
    97321 acgcatgcgc gacctggcag gcggcttcta cattaaaccc gggttttttc gccagcatac
    97381 gaccaaacat agcgatatct accgccatac ggtctttacg taacaaagcg agatctttct
    97441 cttttggcgc gcgcttttct tctgccagtt gatgggccag cgcttttacg gcgtcaaatt
    97501 ctgccgggct gatatggacg agctgttcag tttcggcgct agtgagcgga tcttttggtt
    97561 ttttgtcgtt tttttgtttt ttgtcttttt tggctttccc aagataatcg gctatctcta
    97621 ctgcccattc gatggctttt ttatcttcga ttcctttctc aatcaggata gttgccgcct
    97681 cacgcgcaat acgcccgcta cgaacaccaa tatggcccgc cagcgcctgt tcaaaaagtt
    97741 cagaagtgcg ccaagcacgc ttcagacttt gcgaggaaac gcgcagacgc gttgctccac
    97801 ccagaatcac agttttcgga gagccggaat catcacggtt aagattggca gcagggtaag
    97861 cggttaacaa atgaagctga ataaatgttg tcatgagata gtcctttatt aattttgttc
    97921 gttatcggcg tcgccagcct ggtaatattc cagcgcccag cggatacgga taaattctgt
    97981 tggtcgctgc tggcggcggt gatgattgag caggtcatcg ctctcctggc accagcggaa
    98041 gaccccctct gccagagagt caagattgac ggaaccgttt aataatttga ccgcacggcg
    98101 taactgccgc aataaatcat ccggcgtttt tactgccaac aaacgagtaa accgcccctt
    98161 tttcattacc gccgccagtt gcgcggcaaa aggctgccgt tcgtcaatag ctttaacatt
    98221 ggcgctaact gctgctatca gcgctagtgc ggtgatacgc cactcgggtt catcctgcca
    98281 ctttatttgt ctgttcttaa gaaacaggcg aaatccatcc gtcagacaaa catcattaac
    98341 cgtcgtacta cgccgcagac tggcacgctc gccgcgttta tcctgcaact cttcatgcca
    98401 tttacgcagc gcaactttat gctcatcttt tacaatactc attcagcagc ctcctgcttt
    98461 ttctccctgg cggcgttagc attttgcttc tccgctgatg ttgtaaaata tttcttgcgt
    98521 gcggtcatga cgcgttccaa atcaacgggc tcatatggat tggtaaaggc acgatcgtca
    98581 aaatcctgac gcgcgaataa ccacatttcc ttttgccatt tactgagtaa ttcgtacgga
    98641 tcctgacctt cttcaatttt gcgcactaat ctcaggaatc gatgctgagt tttgttccag
    98701 aagtcaatat ccacaaaacc gaaatcaagc tttgtggttt tcttctcaga gaaccatgct
    98761 tctttcaacg ctcggtgtaa caagcttaga atgtgtgatg ccgcttgcgc tgccagacgc
    98821 aacttaggta ttaggtcttc ttttttgttt aacaacagcg ggaaatggtg ttcataccaa
    98881 caacgaacct tcatatcttc aaaatcaaaa ccaaatcccc ataagcccac ccttgtctgc
    98941 tttagattgc tggcattcaa aagtttcacg acctgtgcgg ggagttcagt attgtttttt
    99001 gagttaccta cctctatgag gcctaaccag tcgcgccaga ttaaaccacc aggttgtggt
    99061 ttaactgagt aaaaatctcc accctcttta agcggcagac ggtaaggtgt tagaggatgg
    99121 cgccacatga cgtattgaac accatagttt ttgagtgaca tcaagcccaa aagagcatcg
    99181 ctctgcctac cgcaaatgtc gcagttacct attgaggttg tcttaaaatc aatacgtata
    99241 cgtcgtggca ttccccagta ggcctgaagt ttattgactt gttcatccgt taccacagca
    99301 ccgtccagtt cgctggtacg cgtcggtgca agccatggaa agaccaggtc atcaaaggtt
    99361 tttggtaaag gtaaatcggc ttcatcttgc ggcattacgt ttagccacag ctttcgccac
    99421 aaaggggttt gttgattgcc ctgatactcc agcaattcta taagtgtcgt cagaggccct
    99481 ccgccacgta gcccaggata gtaaccgcgt ccacctatag gtgcattcaa ctggattgaa
    99541 aaaagcgcta aaggcacaca atgcaggcac agatgctgag ttgtgtctcg cttaataaaa
    99601 tggtctgtat ttcgttcttt tgtttgtttt cctggtgcgt caggaagaag cgaagcaata
    99661 gaagttgcct taacttttaa ttcatcaaaa tcctgcataa atgaagggga atctggccca
    99721 aactgaaaag catgttctaa agacagcaat gcttccctta gcttttcagc ttccagtccg
    99781 tcttcccaga tatcatccca acgacgttga tcttttggcg cgaagctggt ttgtagtagc
    99841 cccaacaaaa actgccaggc cgccccctgg agatctgccc gcggcgcagc gatatcgaca
    99901 acattttcat cagccagatc gactggtgcc agtttgcctg tcgttccgtc tttaaaacga
    99961 acgggcaacc acggggttgt cagaagtgaa aacgagttca tacgcatcct ctcgtaaaaa
   100021 agaccatccc aggtcggttg tccatccttc catctttttc tgtatatcaa ctattcagag
   100081 agtcctgtcg cagacatctc aaccaacctt gccaatcaat ccctccctgg ccgaataccc
   100141 gcaactttcg tcatcggtga ctaaaatcac gtttgccatt tccggatctt gccgctgttc
   100201 aacgcaccac tgcctgaacg catccccttc cagtaaagaa aactcatccc gatgtttttt
   100261 ccaccagctt cggcgcactc tgacaacgct catttcccat gcgtgagcac cggtggcata
   100321 aggcttcacc acaccgtcaa tacaggtagc cagccacagg gaaacagact cttcagccag
   100381 acgtgtcgac agtttttccg gaaggtaatc gttgatattg gcggcatagc cgggcctgaa
   100441 gttcagcaca tactttttag cccttgcgcg atcacagtaa tatttgccca cttgctcctg
   100501 ctcgctgcgg gcaaatcctt ccggcattac cacgtcctca ccgtaaactg attcaataag
   100561 aagccgggct gcgtgtggca tctgaatagc gccttgctca cgcagcacac gctgcgtcag
   100621 ccagattcgc ccatgatcgg gatagacata tgcactgtta cgcatggcac tgccgaacca
   100681 ttcgtcacca ggagcgtcat cccagacggg ggccagaatc agcaattcag ggggggaacg
   100741 ctcgtctttt ccgtcacgct ttaactgacc attaatatcg cggatatgcc gctgtaatcg
   100801 ccccgctcgc tggattagca aatcaatggg ggccaagtcg gagatcattt cgtccaggtc
   100861 aatatccacg ctttgttcaa ttacttgtgt acaaacaatg acttgtgacg ctctctgtaa
   100921 actacaatat ttaccaaagc gtgccagcgt ttccgtttca attcgctggc gatcgctaaa
   100981 agcaaagcgg ctatgaaaaa gtgaaaggct ggaagcagga atgacgccgc gggcaagcag
   101041 ctgacgatac acctgaatag cgtcatcaac agaattccga atccaggcga tgcatttccc
   101101 ctgacttacc gccgattcga tacgcgcaat acactcttgt tcactatgga gccaacccac
   101161 gctcacgcta cgttcaactt ctttgcgcgt cgctacccgg tgcgaatgta catcggtttt
   101221 cgtgacatgc gtcagccagg ggtaatcatc ttttcccagg agtggggctt cttgctggcc
   101281 ctctgcacca cgcgcaaagg cggcgacgag tttgtcgcgc tgctgttgag ataacgtagc
   101341 agaaagcaaa ataacgctgt ttccgccacg cgcctgccgc tcgatcagcc cttcaagaat
   101401 gcacgacatg taagcgtcac aggcgtggat ctcatcagcc agcaggattt tgttactcag
   101461 ccccagaagc cgcagattat tatgtttaaa cggcatcact gccatcatcg cctgatccag
   101521 ggtgccgacg ccaatttcag ccagcaacgc cttcttgtta ctgtcggcaa accaggccgc
   101581 acaaccctgg ctgaaagctt gttcatccgg ttcttctgac ccgattaaat caccggacca
   101641 gagtgattca ttaaagcggt ccattaatgt gcgggcactg tgtgccagca ccaggctggg
   101701 gcgagattct ggcgaataga aagaaagcca ggttttgacc agccgatcgt acatggcatt
   101761 ggccgttgcc attgttggca gaccaaaaaa cagcccctga gctttccccg cagctatcaa
   101821 cctgtgcgcc aggataagcg ccgcttctgt tttacctgcg ccagtcacat cttccagaat
   101881 aaataactgc ggtcctggct ggctgatatc cagatccagc accttttgct gtaacggtgt
   101941 cggattctca ataaaaggaa acagtgtatt aattccggtg aaaggtgcgg tttctgcttt
   102001 tggaggaaag acggttaatg cgttttgagc ctgcactaaa gccttctgcc agtaatgttt
   102061 aatatccatt gcctgtgcga cgcgtggaaa aaatcgcgtt gacgaacctg tccagtctgc
   102121 gagtacgacg gttgcagaga tataccagga aaggtgtttt ataagttcaa tgccctcgtc
   102181 atcatcccag aatgcgggga tctctattcg cggaaacagt gccttgattg caaggagaaa
   102241 atctcgtgct gcagctttgt cttcaggcag aaaattatcc aattcatcca tacgatcagg
   102301 gggccgacca tgatgccctg tcgtcacggg catccacatt tctatcactc gcctaagttt
   102361 gcgcggagag agcgaagatg aaggaagtaa ctcctgacat tcacttaaat aatgattcca
   102421 caaccagtag cccagcgttg aatgagagat tttttcgtaa ttttttctgg cactttcctg
   102481 aatcttgagt tcagggggca ggtaaagttg ttgaaaagag cgggcaaatt ttccaatatc
   102541 gtgccagcac agcaaccagg cgaaaaattg agccgcctgt tccttgtcag aaatccctaa
   102601 ttgacgaaaa taatcagcca gcccgaagca atttctttta accattaaat agcccatcgc
   102661 ggctacatcc agcgaatgcc agcaaagaag gtgatagtcg tcgcccccgt ctttctcgcc
   102721 gcgtcgggtt tttccccaga aatcaaaaaa agtcacaata tttttatcct tcagtaaact
   102781 taaaggatat ttac
//
LOCUS       DAZFYC010000004       102569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFYC010000004 DAZFYC010000000
VERSION     DAZFYC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 102569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.004"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(8..826)
                     /gene="nlpA"
                     /locus_tag="ACHKW5_000332"
     CDS             complement(8..826)
                     /gene="nlpA"
                     /locus_tag="ACHKW5_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO7509780.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            1048..1341
                     /gene="yicS"
                     /locus_tag="ACHKW5_000333"
     CDS             1048..1341
                     /gene="yicS"
                     /locus_tag="ACHKW5_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO7509781.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(1382..2572)
                     /gene="nepI"
                     /locus_tag="ACHKW5_000334"
     CDS             complement(1382..2572)
                     /gene="nepI"
                     /locus_tag="ACHKW5_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO7509782.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2783..3235)
                     /gene="yicN"
                     /locus_tag="ACHKW5_000335"
     CDS             complement(2783..3235)
                     /gene="yicN"
                     /locus_tag="ACHKW5_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO7509783.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3288..4622)
                     /gene="adeQ"
                     /locus_tag="ACHKW5_000336"
                     /gene_synonym="yicO"
     CDS             complement(3288..4622)
                     /gene="adeQ"
                     /locus_tag="ACHKW5_000336"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO7509784.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            4797..6563
                     /gene="adeD"
                     /locus_tag="ACHKW5_000337"
     CDS             4797..6563
                     /gene="adeD"
                     /locus_tag="ACHKW5_000337"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO7509785.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(6608..7999)
                     /gene="uhpT"
                     /locus_tag="ACHKW5_000338"
     CDS             complement(6608..7999)
                     /gene="uhpT"
                     /locus_tag="ACHKW5_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO7509786.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(8137..9456)
                     /gene="uhpC"
                     /locus_tag="ACHKW5_000339"
     CDS             complement(8137..9456)
                     /gene="uhpC"
                     /locus_tag="ACHKW5_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO7509787.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(9466..10968)
                     /gene="uhpB"
                     /locus_tag="ACHKW5_000340"
     CDS             complement(9466..10968)
                     /gene="uhpB"
                     /locus_tag="ACHKW5_000340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO7509788.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10968..11558)
                     /gene="uhpA"
                     /locus_tag="ACHKW5_000341"
     CDS             complement(10968..11558)
                     /gene="uhpA"
                     /locus_tag="ACHKW5_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO7509789.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(11633..11923)
                     /gene="ilvN"
                     /locus_tag="ACHKW5_000342"
     CDS             complement(11633..11923)
                     /gene="ilvN"
                     /locus_tag="ACHKW5_000342"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7509790.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11927..13615)
                     /gene="ilvB"
                     /locus_tag="ACHKW5_000343"
     CDS             complement(11927..13615)
                     /gene="ilvB"
                     /locus_tag="ACHKW5_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO7509791.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(13721..13819)
                     /gene="ivbL"
                     /locus_tag="ACHKW5_000344"
     CDS             complement(13721..13819)
                     /gene="ivbL"
                     /locus_tag="ACHKW5_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO7509792.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            14384..14473
                     /gene="tisB"
                     /locus_tag="ACHKW5_000345"
     CDS             14384..14473
                     /gene="tisB"
                     /locus_tag="ACHKW5_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO7509793.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(14736..14810)
                     /gene="ysdE"
                     /locus_tag="ACHKW5_000346"
     CDS             complement(14736..14810)
                     /gene="ysdE"
                     /locus_tag="ACHKW5_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO7509794.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            14892..16076
                     /gene="emrD"
                     /locus_tag="ACHKW5_000347"
     CDS             14892..16076
                     /gene="emrD"
                     /locus_tag="ACHKW5_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO7509795.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(16084..16581)
                     /gene="yidF"
                     /locus_tag="ACHKW5_000348"
     CDS             complement(16084..16581)
                     /gene="yidF"
                     /locus_tag="ACHKW5_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO7509796.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(16578..16940)
                     /gene="yidG"
                     /locus_tag="ACHKW5_000349"
     CDS             complement(16578..16940)
                     /gene="yidG"
                     /locus_tag="ACHKW5_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO7509797.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(16930..17277)
                     /gene="yidH"
                     /locus_tag="ACHKW5_000350"
     CDS             complement(16930..17277)
                     /gene="yidH"
                     /locus_tag="ACHKW5_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO7509798.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            17386..17835
                     /gene="yidI"
                     /locus_tag="ACHKW5_000351"
     CDS             17386..17835
                     /gene="yidI"
                     /locus_tag="ACHKW5_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO7509799.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(17882..19375)
                     /gene="yidJ"
                     /locus_tag="ACHKW5_000352"
     CDS             complement(17882..19375)
                     /gene="yidJ"
                     /locus_tag="ACHKW5_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7509800.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(19372..21087)
                     /gene="yidK"
                     /locus_tag="ACHKW5_000353"
     CDS             complement(19372..21087)
                     /gene="yidK"
                     /locus_tag="ACHKW5_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO7509801.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKIDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRSTPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLSMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            21254..22147
                     /gene="yidL"
                     /locus_tag="ACHKW5_000354"
     CDS             21254..22147
                     /gene="yidL"
                     /locus_tag="ACHKW5_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7509802.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(22144..22958)
                     /locus_tag="ACHKW5_000355"
                     /pseudo
     CDS             complement(22144..22958)
                     /locus_tag="ACHKW5_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22958..24574)
                     /locus_tag="ACHKW5_000356"
     CDS             complement(22958..24574)
                     /locus_tag="ACHKW5_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7509803.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            24870..25586
                     /gene="yidP"
                     /locus_tag="ACHKW5_000357"
     CDS             24870..25586
                     /gene="yidP"
                     /locus_tag="ACHKW5_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7509804.1"
                     /translation="MIYKSIAERLRIRLNYADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IGQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(25583..27244)
                     /gene="yidE"
                     /locus_tag="ACHKW5_000358"
     CDS             complement(25583..27244)
                     /gene="yidE"
                     /locus_tag="ACHKW5_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO7509805.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(27441..27869)
                     /gene="ibpB"
                     /locus_tag="ACHKW5_000359"
     CDS             complement(27441..27869)
                     /gene="ibpB"
                     /locus_tag="ACHKW5_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO7509806.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(27981..28394)
                     /gene="ibpA"
                     /locus_tag="ACHKW5_000360"
     CDS             complement(27981..28394)
                     /gene="ibpA"
                     /locus_tag="ACHKW5_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO7509807.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(28388..28460)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            28700..29032
                     /gene="yidQ"
                     /locus_tag="ACHKW5_000361"
     CDS             28700..29032
                     /gene="yidQ"
                     /locus_tag="ACHKW5_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7509808.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(29034..30248)
                     /gene="yidR"
                     /locus_tag="ACHKW5_000362"
     CDS             complement(29034..30248)
                     /gene="yidR"
                     /locus_tag="ACHKW5_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO7509809.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            30349..31413
                     /gene="cbrA"
                     /locus_tag="ACHKW5_000363"
     CDS             30349..31413
                     /gene="cbrA"
                     /locus_tag="ACHKW5_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO7509810.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(31410..32735)
                     /gene="dgoT"
                     /locus_tag="ACHKW5_000364"
     CDS             complement(31410..32735)
                     /gene="dgoT"
                     /locus_tag="ACHKW5_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7509811.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVTYFIAIFGW
                     SVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQ
                     FVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKAELDY
                     IRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLTWFPN
                     YLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPIICGLL
                     ISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVFNFAG
                     GLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(32810..33958)
                     /gene="dgoD"
                     /locus_tag="ACHKW5_000365"
     CDS             complement(32810..33958)
                     /gene="dgoD"
                     /locus_tag="ACHKW5_000365"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO7509812.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(33955..34572)
                     /gene="dgoA"
                     /locus_tag="ACHKW5_000366"
     CDS             complement(33955..34572)
                     /gene="dgoA"
                     /locus_tag="ACHKW5_000366"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO7509813.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(34556..35434)
                     /gene="dgoK"
                     /locus_tag="ACHKW5_000367"
     CDS             complement(34556..35434)
                     /gene="dgoK"
                     /locus_tag="ACHKW5_000367"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO7509814.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALTPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(35431..36120)
                     /gene="dgoR"
                     /locus_tag="ACHKW5_000368"
     CDS             complement(35431..36120)
                     /gene="dgoR"
                     /locus_tag="ACHKW5_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO7509815.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            36398..37054
                     /gene="yidX"
                     /locus_tag="ACHKW5_000369"
     CDS             36398..37054
                     /gene="yidX"
                     /locus_tag="ACHKW5_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO7509816.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(37100..37912)
                     /gene="yidA"
                     /locus_tag="ACHKW5_000370"
     CDS             complement(37100..37912)
                     /gene="yidA"
                     /locus_tag="ACHKW5_000370"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO7509817.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(38027..38425)
                     /gene="yidB"
                     /locus_tag="ACHKW5_000371"
     CDS             complement(38027..38425)
                     /gene="yidB"
                     /locus_tag="ACHKW5_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO7509818.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(38665..41079)
                     /gene="gyrB"
                     /locus_tag="ACHKW5_000372"
     CDS             complement(38665..41079)
                     /gene="gyrB"
                     /locus_tag="ACHKW5_000372"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO7509819.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(41108..42181)
                     /gene="recF"
                     /locus_tag="ACHKW5_000373"
     CDS             complement(41108..42181)
                     /gene="recF"
                     /locus_tag="ACHKW5_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO7509820.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAQYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(42181..43281)
                     /gene="dnaN"
                     /locus_tag="ACHKW5_000374"
     CDS             complement(42181..43281)
                     /gene="dnaN"
                     /locus_tag="ACHKW5_000374"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO7509821.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(43286..44689)
                     /gene="dnaA"
                     /locus_tag="ACHKW5_000375"
     CDS             complement(43286..44689)
                     /gene="dnaA"
                     /locus_tag="ACHKW5_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO7509822.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            44986..45066
                     /locus_tag="ACHKW5_000376"
     CDS             44986..45066
                     /locus_tag="ACHKW5_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509823.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            45296..45436
                     /gene="rpmH"
                     /locus_tag="ACHKW5_000377"
     CDS             45296..45436
                     /gene="rpmH"
                     /locus_tag="ACHKW5_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO7509824.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            45453..45812
                     /gene="rnpA"
                     /locus_tag="ACHKW5_000378"
     CDS             45453..45812
                     /gene="rnpA"
                     /locus_tag="ACHKW5_000378"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO7509825.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCHLARGS"
     gene            45776..46033
                     /gene="yidD"
                     /locus_tag="ACHKW5_000379"
     CDS             45776..46033
                     /gene="yidD"
                     /locus_tag="ACHKW5_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO7509826.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            46036..47682
                     /gene="yidC"
                     /locus_tag="ACHKW5_000380"
     CDS             46036..47682
                     /gene="yidC"
                     /locus_tag="ACHKW5_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO7509827.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            47788..49152
                     /gene="mnmE"
                     /locus_tag="ACHKW5_000381"
     CDS             47788..49152
                     /gene="mnmE"
                     /locus_tag="ACHKW5_000381"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO7509828.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            49395..49469
                     /gene="tnaC"
                     /locus_tag="ACHKW5_000382"
     CDS             49395..49469
                     /gene="tnaC"
                     /locus_tag="ACHKW5_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO7509829.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            49690..51105
                     /gene="tnaA"
                     /locus_tag="ACHKW5_000383"
     CDS             49690..51105
                     /gene="tnaA"
                     /locus_tag="ACHKW5_000383"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO7509830.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            51196..52443
                     /gene="tnaB"
                     /locus_tag="ACHKW5_000384"
     CDS             51196..52443
                     /gene="tnaB"
                     /locus_tag="ACHKW5_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO7509831.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            52575..53750
                     /gene="mdtL"
                     /locus_tag="ACHKW5_000385"
     CDS             52575..53750
                     /gene="mdtL"
                     /locus_tag="ACHKW5_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO7509832.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            53725..54684
                     /gene="yidZ"
                     /locus_tag="ACHKW5_000386"
     CDS             53725..54684
                     /gene="yidZ"
                     /locus_tag="ACHKW5_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO7509833.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            54841..55590
                     /gene="yieE"
                     /locus_tag="ACHKW5_000387"
     CDS             54841..55590
                     /gene="yieE"
                     /locus_tag="ACHKW5_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO7509834.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            55612..56178
                     /gene="yieF"
                     /locus_tag="ACHKW5_000388"
                     /gene_synonym="chrR"
     CDS             55612..56178
                     /gene="yieF"
                     /locus_tag="ACHKW5_000388"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO7509835.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(56232..57569)
                     /gene="adeP"
                     /locus_tag="ACHKW5_000389"
     CDS             complement(56232..57569)
                     /gene="adeP"
                     /locus_tag="ACHKW5_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO7509836.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            57736..58401
                     /gene="yieH"
                     /locus_tag="ACHKW5_000390"
     CDS             57736..58401
                     /gene="yieH"
                     /locus_tag="ACHKW5_000390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO7509837.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            58468..58941
                     /locus_tag="ACHKW5_000391"
     CDS             58468..58941
                     /locus_tag="ACHKW5_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509838.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            58990..59577
                     /gene="cbrC"
                     /locus_tag="ACHKW5_000392"
     CDS             58990..59577
                     /gene="cbrC"
                     /locus_tag="ACHKW5_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO7509839.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(59639..60361)
                     /gene="yieK"
                     /locus_tag="ACHKW5_000393"
     CDS             complement(59639..60361)
                     /gene="yieK"
                     /locus_tag="ACHKW5_000393"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7509840.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(60376..61545)
                     /gene="yieL"
                     /locus_tag="ACHKW5_000394"
     CDS             complement(60376..61545)
                     /gene="yieL"
                     /locus_tag="ACHKW5_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7509841.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(61572..63188)
                     /gene="bglH"
                     /locus_tag="ACHKW5_000395"
     CDS             complement(61572..63188)
                     /gene="bglH"
                     /locus_tag="ACHKW5_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO7509842.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNAIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(63274..64668)
                     /gene="bglB"
                     /locus_tag="ACHKW5_000396"
     CDS             complement(63274..64668)
                     /gene="bglB"
                     /locus_tag="ACHKW5_000396"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO7509843.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(64687..66564)
                     /gene="bglF"
                     /locus_tag="ACHKW5_000397"
     CDS             complement(64687..66564)
                     /gene="bglF"
                     /locus_tag="ACHKW5_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO7509844.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTRFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(66696..67532)
                     /gene="bglG"
                     /locus_tag="ACHKW5_000398"
     CDS             complement(66696..67532)
                     /gene="bglG"
                     /locus_tag="ACHKW5_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO7509845.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(67818..68543)
                     /gene="phoU"
                     /locus_tag="ACHKW5_000399"
     CDS             complement(67818..68543)
                     /gene="phoU"
                     /locus_tag="ACHKW5_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO7509846.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(68558..69331)
                     /gene="pstB"
                     /locus_tag="ACHKW5_000400"
     CDS             complement(68558..69331)
                     /gene="pstB"
                     /locus_tag="ACHKW5_000400"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO7509847.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(69422..70312)
                     /gene="pstA"
                     /locus_tag="ACHKW5_000401"
     CDS             complement(69422..70312)
                     /gene="pstA"
                     /locus_tag="ACHKW5_000401"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO7509848.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(70312..71271)
                     /gene="pstC"
                     /locus_tag="ACHKW5_000402"
     CDS             complement(70312..71271)
                     /gene="pstC"
                     /locus_tag="ACHKW5_000402"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO7509849.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(71357..72397)
                     /gene="pstS"
                     /locus_tag="ACHKW5_000403"
     CDS             complement(71357..72397)
                     /gene="pstS"
                     /locus_tag="ACHKW5_000403"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO7509850.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(72711..74540)
                     /gene="glmS"
                     /locus_tag="ACHKW5_000404"
     CDS             complement(72711..74540)
                     /gene="glmS"
                     /locus_tag="ACHKW5_000404"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO7509851.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(74702..76072)
                     /gene="glmU"
                     /locus_tag="ACHKW5_000405"
     CDS             complement(74702..76072)
                     /gene="glmU"
                     /locus_tag="ACHKW5_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO7509852.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVFGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(76423..76842)
                     /gene="atpC"
                     /locus_tag="ACHKW5_000406"
     CDS             complement(76423..76842)
                     /gene="atpC"
                     /locus_tag="ACHKW5_000406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO7509853.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(76863..78245)
                     /gene="atpD"
                     /locus_tag="ACHKW5_000407"
     CDS             complement(76863..78245)
                     /gene="atpD"
                     /locus_tag="ACHKW5_000407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO7509854.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(78272..79135)
                     /gene="atpG"
                     /locus_tag="ACHKW5_000408"
     CDS             complement(78272..79135)
                     /gene="atpG"
                     /locus_tag="ACHKW5_000408"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO7509855.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(79186..80727)
                     /gene="atpA"
                     /locus_tag="ACHKW5_000409"
     CDS             complement(79186..80727)
                     /gene="atpA"
                     /locus_tag="ACHKW5_000409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO7509856.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(80740..81273)
                     /gene="atpH"
                     /locus_tag="ACHKW5_000410"
     CDS             complement(80740..81273)
                     /gene="atpH"
                     /locus_tag="ACHKW5_000410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO7509857.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(81288..81758)
                     /gene="atpF"
                     /locus_tag="ACHKW5_000411"
     CDS             complement(81288..81758)
                     /gene="atpF"
                     /locus_tag="ACHKW5_000411"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO7509858.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(81820..82059)
                     /gene="atpE"
                     /locus_tag="ACHKW5_000412"
     CDS             complement(81820..82059)
                     /gene="atpE"
                     /locus_tag="ACHKW5_000412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO7509859.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(82106..82921)
                     /gene="atpB"
                     /locus_tag="ACHKW5_000413"
     CDS             complement(82106..82921)
                     /gene="atpB"
                     /locus_tag="ACHKW5_000413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO7509860.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(82930..83310)
                     /gene="atpI"
                     /locus_tag="ACHKW5_000414"
     CDS             complement(82930..83310)
                     /gene="atpI"
                     /locus_tag="ACHKW5_000414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO7509861.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(83927..84550)
                     /gene="rsmG"
                     /locus_tag="ACHKW5_000415"
     CDS             complement(83927..84550)
                     /gene="rsmG"
                     /locus_tag="ACHKW5_000415"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO7509862.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(84614..86503)
                     /gene="mnmG"
                     /locus_tag="ACHKW5_000416"
     CDS             complement(84614..86503)
                     /gene="mnmG"
                     /locus_tag="ACHKW5_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO7509863.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(86882..87325)
                     /gene="mioC"
                     /locus_tag="ACHKW5_000417"
     CDS             complement(86882..87325)
                     /gene="mioC"
                     /locus_tag="ACHKW5_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO7509864.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(87415..87873)
                     /gene="asnC"
                     /locus_tag="ACHKW5_000418"
     CDS             complement(87415..87873)
                     /gene="asnC"
                     /locus_tag="ACHKW5_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO7509865.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            88025..89017
                     /gene="asnA"
                     /locus_tag="ACHKW5_000419"
     CDS             88025..89017
                     /gene="asnA"
                     /locus_tag="ACHKW5_000419"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO7509866.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(89022..90473)
                     /gene="viaA"
                     /locus_tag="ACHKW5_000420"
     CDS             complement(89022..90473)
                     /gene="viaA"
                     /locus_tag="ACHKW5_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO7509867.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALLTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(90467..91963)
                     /gene="ravA"
                     /locus_tag="ACHKW5_000421"
     CDS             complement(90467..91963)
                     /gene="ravA"
                     /locus_tag="ACHKW5_000421"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO7509868.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEIDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            92186..94054
                     /gene="kup"
                     /locus_tag="ACHKW5_000422"
     CDS             92186..94054
                     /gene="kup"
                     /locus_tag="ACHKW5_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO7509869.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            94221..94640
                     /gene="rbsD"
                     /locus_tag="ACHKW5_000423"
     CDS             94221..94640
                     /gene="rbsD"
                     /locus_tag="ACHKW5_000423"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO7509870.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            94648..96153
                     /gene="rbsA"
                     /locus_tag="ACHKW5_000424"
     CDS             94648..96153
                     /gene="rbsA"
                     /locus_tag="ACHKW5_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO7509871.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGLGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            96158..97123
                     /gene="rbsC"
                     /locus_tag="ACHKW5_000425"
     CDS             96158..97123
                     /gene="rbsC"
                     /locus_tag="ACHKW5_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO7509872.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            97148..98038
                     /gene="rbsB"
                     /locus_tag="ACHKW5_000426"
     CDS             97148..98038
                     /gene="rbsB"
                     /locus_tag="ACHKW5_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO7509873.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            98164..99093
                     /gene="rbsK"
                     /locus_tag="ACHKW5_000427"
     CDS             98164..99093
                     /gene="rbsK"
                     /locus_tag="ACHKW5_000427"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7509874.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALTPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            99097..100089
                     /gene="rbsR"
                     /locus_tag="ACHKW5_000428"
     CDS             99097..100089
                     /gene="rbsR"
                     /locus_tag="ACHKW5_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO7509875.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(100055..101482)
                     /gene="mdtD"
                     /locus_tag="ACHKW5_000429"
     CDS             complement(100055..101482)
                     /gene="mdtD"
                     /locus_tag="ACHKW5_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO7509876.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(101505..102197)
                     /gene="yieP"
                     /locus_tag="ACHKW5_000430"
     CDS             complement(101505..102197)
                     /gene="yieP"
                     /locus_tag="ACHKW5_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO7509877.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        25255 a        26911 c        25696 g        24707 t
ORIGIN      
        1 gtgagaatta ccagccaggc accgcgccac cgttaaaaat ggtttctgcc gctttagcga
       61 cttcgggtga ttgataagat tgcagaaatt ccttcacgtt ttctgcattc ttattatctt
      121 cccgtgccac caaaatattc acatacggcg aattcttatc ttcaataaat acgctgtcgt
      181 gcaccggaga aagccctgtc tgctgaatgt aagtggtact gataatcgct acatcaactt
      241 tgggatcatc cagtacgcgc ggaagctgcg ccccctcgag ttccataatc tgcaaatgac
      301 gaggattatc ggttatatcc agcgcggtag gtaataaccc ttttccctct ttcagagtaa
      361 ttagcttctc tttttgcaac agtaaaagtg cgcgaccaag gttggtcggg tcgttgggga
      421 tcgccactgt cgcgccttct tttatttgcg ccacggtttt gatttttttg gaataacccg
      481 ccatagggaa gacaaacgta tttcccaccg ctaccagttt ataaccatgc gcctgattat
      541 cttgttcaag aaagggtcga tgctggaaga cattcgcatc aagttcgcca tgatttgtcg
      601 catcgttcgg tagcaacgaa ccgctaaatc ctaccagttc aacatcgagg ccatatttct
      661 cttttgccac ctttttagcg acttccgcga catcttgttc tgcgccattt ataacgccaa
      721 ctttaatgtg ttttgcatcg ctgctactct ggtcgcaacc tgccagcaga attccggcca
      781 gcaataatgc ggcccctgtc cgtagatgat gtgttgtcag tttcattttt ttatcctttt
      841 attaatgatt acctgactat agcggtaagg tcgctgcggt ttaaaaaacg aaacgctatc
      901 gataagaata aaaaggaata aaagtggaat ataaagtctg gcacttctta ctgaccacgc
      961 aagcgcgttt cgtacagcat gatgagagcg atgaaagcaa actacactta tgcttcattc
     1021 gatacacatt tgtaaaaggt tgaatccatg aaaccaacga cactactgct tattttcact
     1081 tttttcgcta tgccaggcat cgtatacgcc gagtcgcctt ttagctcact gcagtccgca
     1141 aaagagaaaa ccacggtatt acaagattta cgtaaaattt gcacgccaca ggcgtcatta
     1201 tcagatgaag cgtgggaaaa gttaatgttg tcagatgaaa acaataaaca acatatacgc
     1261 gaggccatcg tggcgatgga acgtaataat cagagcaatt attgggaagc gttgggtaag
     1321 gtagaatgcc cggatatgta agaacagcaa caactactga ccaacgcttc tttacctgat
     1381 atcaggattt cttcattttc acctttgcag taaccaacaa tgcagtcagc aacatcaatg
     1441 tgccggacaa catcaacggc gaagtcagac caatattatc cagcgcataa ccgccgattg
     1501 ccgcgccaca ggtattagca agctgaataa ccgccacctg aatagacccg gctttttctg
     1561 cctgatcggc cagcgagcgg gtgatccacg ttgaccagcc gacgggaacc aatgcaaaag
     1621 ttagccccca gataatcgcc acgccggtag caacgatttt atcgcttccc cacaacgtca
     1681 gtaccaacgc actcacagcc agtattaacg gcgcgcctgc taaggccagt tttaccgaac
     1741 gtttaagaat gaatgacgaa agcgacgtac cgataaagct ggcaatgcca aaactcaaca
     1801 gcaccagcgt taagccatcc acgctgaatc ccgccaggtt catatacacc gggcgaatat
     1861 atgtgaagaa agcaaactgc ccggcgaaag acatgaagat ggcgatcatc cctgccatca
     1921 cacccggacg ttgtaataag cggaaagtat tttgtttctg atgcgagggt tcgcctggca
     1981 gtgaaggcaa tgatttgata atccagaaaa tacacagcac gcccatcacc gccgccgcat
     2041 taaagacatt gcgccaaccg ataagctcgc ctaaaaaact gcccaacggc gcggcaatca
     2101 ccagcgcaat agaaaccgcg ccgaagatca ccgacagcgc cttcggcacc gtacgcggcg
     2161 gcaccagacg catggtcagc gacgccgaca tcgcccagaa cccgcccagc gccagcccca
     2221 gacaggcacg accgattaaa agcaaactga atgagttagc aaaggaaacc agcaagcagg
     2281 agagcgtcag caaaacggca aacaaaataa caacgtagcg gcggtcagta gcctgaattg
     2341 tctgggtaat aaacaaactg gcaaacattg ccacaaaggc ggtcacggtc accgattgcc
     2401 cggcaacccc ttccgaaatg cctaaatcct gggccattgg cgtcaacaaa ctgacgggca
     2461 aaaactcaac gataatcaga caggcgacac aaaacgccac cgagaaaacg gctgaccagt
     2521 tcggtcgggt gatggcatcc gcgccgcggt tttcggcaat aaattcactc atggtgttac
     2581 ccgtggcatg tttcagggga aaagccgacc actttaacat tgaaatgtgt gacgcactta
     2641 acgtttttgc aacttttacg gtgttgcttg agctggagtt aagatcgaaa cggaacaggg
     2701 caaagattac cggatgcggc gtgaacgtct tatccacccg acaggtcagg cgcggtctgt
     2761 aggctgataa gacgaagcgg ttttaatgca gccagtgaac gccctcggca ggctgaaaga
     2821 acgcgttata gcgcatctga aaagcattaa tatcctgcat ttcaggttcg gtttcgcgca
     2881 gaaaacccag cgccagcctg acctgagcat cggtaatcgg cgcggcaaga cgggcttttt
     2941 gtaaaatccg atgccattgc agcaggttct gttcgctacc atcgacaatg acaatttgcc
     3001 agatgagcaa cacctgcgcg gcgttttgca ggtgcgactc atccgggcga tgcaaataac
     3061 gtaaaaattc gtcaccggct gactttccca tttgatcgac catcgactcc acgggtacga
     3121 catcgatatt cagccgatcg ctgagacggc gaatcgcttt tctggccccg gatgtcagca
     3181 cccgaaaacc aaccacaaac actaccgcca gcgtggcgag cattatccag atcatgagtt
     3241 ctcctgcgta atcagaactc atgatatctg gaaaccctcg ccagttacta atccaccaga
     3301 ataatcttca gtgcaaacag agctgcgacc accaccacac acaggttcag atcgcgccag
     3361 cgcccggtgc acactttcat gatgcagtac gacataaagc cgagtgcaat cccttcggtg
     3421 atcgaaaaag taaagggcat catcaccgtg gtaataaacg caggcaccga ttcggtaaaa
     3481 tcatcccagt taacgcgcgc caggctcgaa gtcatcagca cgccaacaaa gattaacgct
     3541 ccggcggttg cgtaaggagg aaccatcgcc accagcggtg agaagaacat aactaacagg
     3601 aacataacgc caaccacaac cgcagtcagc cccgtgcgac caccgactgc cacaccagaa
     3661 gtactttcaa tataggcagt aacagacgag gtgccgataa acgcccccgc caccgaactg
     3721 acgctatcaa catacagcgc cttattcata ttggggaatt taccgttact atctattaag
     3781 cccgctttat cagttacacc aattaatgtt cctgatgaat caaataggtt gatcagcata
     3841 aaggagaaaa tgataccggc gagttcaagt gttaacgcgc cgctcaaatc tacttcacca
     3901 atgacgccgc taatatcagg cggaatggaa tagacgccgc taaaatgaac atcaccgaaa
     3961 aataatccac agcaagacgt caccacaata gaaaccagca ccgcggcatg aaaatgacgt
     4021 gatgacaaca cggttataat aaaaaaccct aaaataccta acaacacgcc gtgagaactt
     4081 aaatcgccaa tcatcaccag cgtgtcttta ttggcgacaa taacgccagt attttttaat
     4141 cccattaagg cgataaataa tccaattccg ctggtgatac caatacgtaa acttaaggga
     4201 atgttggaga tcatccagta ccggatacga aacagcgtga gcaaaaatag tccaactgcg
     4261 ccccagaata ttgcgcccat cccggtctgc caggagatgc ccatcgcccc cacgaccacg
     4321 aaggcaaaga aggcgttcag ccccattgcc ggagccagcg ccacgggtaa gttagcaaat
     4381 atccccatcg caatactgcc gataccggca atcaaacagg tggtaacaaa caccactttc
     4441 gggtccattt gtgccgcgcc gaggatttgc gggttcacaa aaacgatgta caccatggtt
     4501 aaaaaagtgg tcatccccgc aatcaattct gtgcggacgg tggtcccatg ctgaggtagt
     4561 ttaaataatc gcgaaagcgt ccctgattca ttactcacgt aatcggtatt gtcattattc
     4621 atttttttgt ccattggagg agatttaatc ccttgctttt gtaaaacaaa tgacaacaac
     4681 ggttcagtga taattatcac atttcaattg cacattaatg gatattcttt aataatctcg
     4741 cgacgtttct ttatgataaa taataatcaa attgataaaa tcaaaatgag aaaaatatga
     4801 ataattctat taaccataaa tttcatcaca ttagccgggc tgaataccag gaattgttag
     4861 ccgtttcccg tggcgacgct gttgccgatt atattattga taatgtctct attctcgacc
     4921 tgatcaatgg cggagaaatt tccggcccaa ttgtgattaa aggacgttac attgccggtg
     4981 ttggcgcaga atacgctgat gctccggctt tgcagcggat tgatgctcgc ggcgcaacgg
     5041 cggtgccagg gtttattgat gctcacctgc atattgaatc cagcatgatg acgccggtca
     5101 cttttgaaac cgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa
     5161 tcgtcaacgt gatgggagaa gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa
     5221 ggcaaaacca gtacttacag gtcagctctt gcgtacccgc cctggaaggc tgcgatgtta
     5281 acggtgccag ttttaccctt gaacagatgc tcgcctggcg ggaccatccg caggttaccg
     5341 gccttgcaga aatgatggac taccctggcg taattagcgg gcagaatgcg ctgctcgata
     5401 aactggatgc atttcgccac ctgacgctgg acggtcactg cccgggtttg ggtggtaaag
     5461 aacttaacgc ctatattact gcgggtattg aaaactgcca cgaaagttat cagctggaag
     5521 aaggacgccg gaaattacaa ctcggcatgt cgttgatgat ccgcgaaggg tccgctgccc
     5581 gcaatctcaa cgcgctggca ccgttgatca acgaatttaa cagcccgcaa tgcatgctct
     5641 gtaccgatga ccgtaacccg tgggagatcg cccatgaagg acacatcgat gccttaattc
     5701 gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt
     5761 cgacggcgcg ccactttggt ctgaatcacc tcggcttact ggcacccggc aagcaggccg
     5821 atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg
     5881 agccgattga tgcgcaaacc ttacaggcgg aagagtcggc gagactggca caatccgctc
     5941 cgccatatgg caacaccatt gcccgccagc cagtttccgc cagcgacttt gccctgcaat
     6001 ttacgcccgg aaaacgctat cgggtcattg acgtcatcca taacgaattg attacgcact
     6061 cccactccag cgtctacagc gaaaatggtt ttgatcgcga tgatgtgagc tttattgccg
     6121 tacttgagcg ttacgggcaa cggctggctc cggcttgtgg tttgcttggc ggctttggac
     6181 tgaatgaagg tgcgctggct gcgacggtca gccatgacag ccataatatt gtggtgatcg
     6241 gtcgcagtgc cgaagagatg gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc
     6301 tgtgcgtggt acgtaacggc caggtccaaa gtcatctgcc gttgcccatt gccggcctga
     6361 tgagcaccga cacggcgcag ttgctggcgg aacaaattga cgccttgaaa gccgccgccc
     6421 gtgaatgcgg tccgttaccc gatgagccgt ttattcagat ggcgtttctt tctctgccag
     6481 tgatccccgc gctaaaacta accagtcagg ggctatttga tggcgagaag tttgccttca
     6541 ctacgctgga agtcacggaa taataaaaaa gcccggcgtc atgccgggca aaagtcacca
     6601 gttacgttta tgccactgtc aactgctgaa tttttttctc gcggcggatt ttgcgttctt
     6661 ccatcaccgc cactatcgcc atcaggcaga tacaaccaat cgcggcgata tcaagtgcgg
     6721 cgaaggtgcc tgcccagccg gtaaggccga ataccggcgt cccatcggca atcattccca
     6781 gacctaactt ggcaaagctg tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg
     6841 cagcgccaat cgctttttta ggtacaaagc caacagcagc cacaccaatc aacaattgcg
     6901 ggccaaagac caggaaaccc aacgcaaaga gagaagccag atagatatat tcgttgctgg
     6961 catgttgata cacaccgagc gtggcgataa tcagcgccag cgcgatgcag gccaccaggc
     7021 cacggcgacc gttcgccagg tcagagagcc agccccacag cagcgtaccg accagcgcac
     7081 cagcttcaaa cagcgtaaag ccctgaatcg ccaccgcttt agagagtttc agttcctgga
     7141 acgcgtatac ggttgaccac tggtcgatac caatacgtac cacatagagg aaaatgttgg
     7201 cgaagcacag cagccagatc actttgtttt tcagcacata ctcaacaaag atctgccact
     7261 tggtcatatc ggtagattct gtctctttgt cctcttcgct gatctcctcg ccgaacagtt
     7321 cttcagcttt gccgaggcca taagattccg gggagtcgct gccgtaacgc aggccgataa
     7381 aaccgacaat cagcgcgata atcgacggga agataaacat gccgatgaca tggccatcga
     7441 acaggtaatt tgccccgaac agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag
     7501 aaatattcca gaaaccgagg aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg
     7561 tggagtaact gcacgaaccg ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa
     7621 tcatcaggaa caggctaacc gagccgctgc ccatactggc actgaagccc agcatacaaa
     7681 tggcagacag gatcagcatg aacggcagga attgtttggt gtttttgccg tcggcgtagt
     7741 aggaaaccag cgttttaccc acgccataag tgatggagaa acccaggccg atcatcccca
     7801 gctgcgtcat gctcaacccg taggtcgaga tcatgtcgtt ctgcgcgatg ttaaagttct
     7861 tgcgaatcag gtacatcgtc aggtagccga taaagaccac caggtaggat tgcatgaacg
     7921 gtttgaacca cattttgcgc cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct
     7981 ggtttaagaa agccagcatg ggttactcct gaaatgaata cctgcgtgag gcatgcattg
     8041 taaaaatcag cagcaaatca ggcctgagat agcgtccagg caaaacctgg aaaatttctt
     8101 agttttgccg gatatgaagt gaaaaggtga gatgcatcac gcttcgcgcg gtgcctgggc
     8161 gttcaaaaag ggcagtaaca gcagtgcgga aatcccggcg gcgatagaga taaccacaaa
     8221 aaatccgctc cagtgccagg tatcgagtac tttcgccagc ggccaaccag caagcgacgc
     8281 ccccagataa gcaaacaagc cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga
     8341 acactctgcc gccgccatgc cgattaacat ctgcgggcca aagacaaaaa aaccaatggt
     8401 gaagaagcag gttgcctgca tcacgtagct ggcaaatggc atcagccaca gcgagccgac
     8461 tgaaagcaaa attccggcgg cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa
     8521 tttgtccgag ccccaaccgg ctaccagcgc accgataaat ccgcccagtt caaacatcgt
     8581 cactgccgta ttcgccgtga ccagatcgac gcccagtgtc tcggacatat acaaattgcc
     8641 ccagtcgttg atcgccgccc ggaccacata gaccagcaca tagcaaaacg aaagcagcca
     8701 gatatacgga ttcagcaaca catatttggt gaggatctct ttacgcgtca accctgcccc
     8761 ttcttgttgt tgagcaattt ccagcgcgtc gtgtcgccat tcaccgaccg ccggtaaacc
     8821 taacgcctgc gggcgatcgc gtagccgcca gcagagaaaa atccccacga ctatcgccat
     8881 acaaccagca atcatcatcc cggcacgcca gccgtaatgc agcgcagccg ctgccatcac
     8941 aatgggaatg agtgcgccgc cgacgttatg cgccgtgttc cataatgccc accaaccgcc
     9001 gcgctcggta cgtgaatacc aggccgttaa cagacgcgca cacaccggtg aaccccagcc
     9061 ctggaaaaag gcgttcagca cccagagcac ggcaaacgcc catagcgacg tcgagaagcc
     9121 aaacagaatg ttgatgatgc ccgtggcgat aagccctatc cccataaaat aacgggcatt
     9181 tgagcgatcg ctgacaatgc cggagacaaa cttcgacacg ccgtaggtaa tgtaaaacag
     9241 ggtcgctaac aggccgatat cgctacggct gagcacgccg ttagcaagga tttctggtac
     9301 ggcggcgtta aaacttttac gcgtgaagta aaacagcgcg taacccagcc agatggtcag
     9361 cagaatatgc cgacgccagt agcgatagcg ggcatcaatt tcatatttat cagtcattaa
     9421 tggcgcatcg gcaggcgctt tcagaaacgg caacatcatc aaaccttaga catagcgttg
     9481 aggtagagaa acgctgacac gcgtgccgtg cagacaggaa atgtgtaatg tgccacccag
     9541 cgccgttacg cgctcgcgca ttccggtgag gccaaaacct tgttgcccgg aacccggcgg
     9601 caaaccgctg ccatcgtctt caataaccag catcaaccgt tcatcctgct gccagccttg
     9661 cagcgtgacc gcgctggcat cagcatgttt cacaatgttg ttcagccctt cctggcagac
     9721 acgaaacagc gtcacgcgct ggttttcact taatgctgat tcatcgattc gccattcgag
     9781 atggctgaca ataccgcgcc cttccagctc catttcccgc atcagtgagc ggatggcctg
     9841 ctccagggtg agatcatcca actggcgcgg acgtaaccga cccaacaaac ggcgcaccgc
     9901 gtcgtaaacg cccagcgata gttgttcgat gagctgcccg ctctgcttca cgctggcgtt
     9961 atctgccgcc agccgctgaa caatgcccgc ctgagtacga atagcagtga tggtctgacc
    10021 gatatcatca tgcagctcac gcgccacatc acgacgcacg ctctcttcgg tttccagcaa
    10081 ccgttcagcc agatgctgat tgcgcgccag ttccttttgc agcgactggt taagttcacg
    10141 caaccgctgg atgccagcgc ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag
    10201 caataaatcc accggatgat cgcgccaggt ttgactggcg atcagcgcga tggcgttcat
    10261 caacgtcgcg atcagcgccc cttgccaacc gtagtgccag gccagcgcga taatcggcag
    10321 cgccaggcag aatggcgtaa agcgcgacag ttcgtccggc aatcccaact ggagccagag
    10381 actaataaca aacaacagca agtaccagac cagatgccgc ccgcgccagt tgatcggttg
    10441 agaaaccagt gacgggccga gcggcagcca ggtgttattg gcgagatagt gccagaacac
    10501 cagacatatc ggggccagcg tcaggccgcc agttaaagtc agcaacagcg cattccacga
    10561 ctctttgccg tgccaaagcc agggcagcga ctgcaacaac gccgccgccg ttaacgccgc
    10621 cccctgtaac agcaaggtgc gccagtcacg ctgatggcga tagcgcgaga tcagcgctac
    10681 gggcagtaac gtcagtaaac taccgatcat caataacgga aaatgggtta aaccgaccgc
    10741 ctgcgttagc cagtaaatca gcagccattc cgcacccagc aatacgggcc agtatccgcg
    10801 cgggcattgc agcattagcc ctagccgcag accaaacgga aataacagca ccgccatatc
    10861 agggcgctca accaggtgca ggctgatact ccacaggcaa aaccatgcgg cagagaagat
    10921 aaaaaagcag gcaataacgg taattaagcg ggagaacaac gtcttcatca ccagccatca
    10981 aacatgcggc gcgccagttc tacgtcgtta ctgacgccca gtttttccat cagattggcg
    11041 cgatggacgt gtaccgtttt cggtgacaag cccagttcgg cggcaatctc tttcaccgcc
    11101 attccttgcg ccagtttttc cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga
    11161 ccggatgcca gtttaatggc aatatccggc gtcagataac agccgcccgt agcaaccgta
    11221 tgcaccgccg caatcagttc gtcagggcta cagcgtttgg agagaaagcc gcgcgccccc
    11281 gcgttaagcg cctgctcaac cagcgccgga ctgtcatgaa cagagagcat tattgtcgcc
    11341 atacctttcg gcagctggct tagcagttcc agaccggaga tatcgggcat ggagatatcg
    11401 caaatacaca cctgcacacc gcgccccggc agccccgcca gcgcctcgcg ccccgaacca
    11461 aactcggcaa ctacctgcaa atcaggttcc agccccagca gctgcgcaaa gccggagcgg
    11521 acgatgaggt gatcgtctat aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa
    11581 aaaaacgcgc ttaccttaac gataagcgcg atgttgttca agccttgagc ggttactgaa
    11641 aaaacaccgc aatcttgtta aacatagtcg gatcggactg attacgctgc actttcacca
    11701 catcttccag cttatcgatt tggcttatca tctgctccag acgctggtcg tcattgacca
    11761 gtagccagat atggcttttg tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt
    11821 taaaagcgcg gcgggcaaaa aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa
    11881 cggtgagctc cagaatcacg ttgtcatgag ttgtgttttg catggcttat tcccccacca
    11941 tttcagtatt cgccgcacct ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc
    12001 gcacatggat cagcgccggg ccagggcgat tgatgatttc ctgcaatgca gcctgcggat
    12061 cggcttcgtt attcaaatca caggtttcga ggccgaatcc ggcggcaatc tgcataaagt
    12121 tgatttttcc cggataggtg gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct
    12181 gatgcaccag ccccagcgct tcgttgttca tcagaatgat tttgacatcc agctgatttt
    12241 cactggcggt cgccatctcc tgaatattca tcatcaggct gccgtcgccg gagaaacaca
    12301 acactttgcg atccgggttc gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca
    12361 tcgtgcccag cccaccggag gtcagccact ggcgtgggcg attgagcgga taagcttgcg
    12421 cggtccacat ctgatgctga cccacatcgg tggtgataat cgcattgtca tcgacacagg
    12481 cggcaacggc gttgatcagg ccgtaatggc ttaatggatc gcacgctttc gggattggac
    12541 acgggaattc gcgctgcaaa tccgctacca actggtgcca ctctgcacgc ggttgcgctt
    12601 ccacctgcgg gatcaattgc gccagcacgt catcaacatc cgcctgaatc gccacatgcg
    12661 gctgcttgat tttacccagc tctgcgcgat cgatatcgac atgaataatt ttggcattcg
    12721 gacagaactg ctcggttttg ccaatcgccc ggtcatcaaa acgcgcaccg agcactatca
    12781 acaaatccgc ctcctgcaaa atatagttgg tgctgcgcac gccgtgcatc cccagcatac
    12841 ccagcgacaa cggatgcgct tttggcaaca tgcccagcgc cattaaagtc atggtggtag
    12901 gcagttgcgc tttctccgcc agttcacgca cccgtgcggg cgcattgatc acaccgccgc
    12961 ccagataaag caccgggcgt ttggcagcgt taatcatcgc cgctgcgtca cgaatgcttt
    13021 cttcgctaaa ggcgggggcg gcggcttttt ctgccatagc gggctgtgtt tcaatctcaa
    13081 aaactgccgt ttgcacatcc ttaggaatgt ctatccacac cgggcctggg cggcctgatt
    13141 gcgcaatgcg gaaggcatcg ctcatgacct gcgggagttc ttcgatatgt ctgaccagat
    13201 agttgtgttt ggtgatgggg atagagatgc cgtaggtgtc cacttcctgg aaggcgtcgg
    13261 tgccgatcat cgaggcggga acctgaccgg tgatgcaaat cagcgggatg gagtccagcc
    13321 gcgcatcggc aatggcggtc accaggttag tcgcacccgg tccgctacag gccatacaga
    13381 ccgccggttt accgtcggtg cgcgccatcc cctgagcgat aaatcccgcg ccctgttcat
    13441 gacgggccag aatatggcgg atttgcgtgc tttggcttaa ggcatcgtaa acaggcagga
    13501 tagaaccgcc cggaatgccc gtcacaatct taatgccctg ctgttccagg aaatgaacga
    13561 taaattctgc gccggtaaag cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc
    13621 agtccttttc ttctgggccg actttccggg aggtgcttaa acgaaaaacc ccgcccggtt
    13681 tgcgccggcg gggttttgga atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga
    13741 ccaccaccac acgcacgacg accactgcgg cggatagcgc agttggtagt agttttgcgt
    13801 tgaacatgga agtagtcatt ggggaccttg ttggttttgt gtttaacaat atttatacaa
    13861 gcacagcttt acaggggaga caatggaaaa tttttcagca agggaaaatt gaggggctga
    13921 tcacgttttg tactgaattg cagataacaa aaaaccccgc cggagcgagg tttcatcagc
    13981 cgcctgcggc tggtaaccgc aaagcacact gtattatgtc aacactgaaa gtatacgtgt
    14041 tccgcgcaga acgcgcaatt tcggcacgaa ttttgacgta tttagggcat agttgagtat
    14101 cgatcacagt ttgcgttttg tccaaatatt actgtttatt tatacagtaa acttctataa
    14161 tatcactgta cgcaatgtgt tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg
    14221 gctgaaacgg gtggtgccgt cagcgcctta accccgcgtg agcacactgt gttatgtcaa
    14281 caagcacaac gtttctcctt aagataccgc gtgcacaaca gctggcaaca ggcagcggaa
    14341 aggtacgtca gctggcagtg ctcctgaacc acaggagacg cgtatgagcc tggtggatat
    14401 cgccattctt atcctcaaac tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa
    14461 atacctgaag taattcagat tcaagtcgca ccaaagggga gcgggtaacc gctccccttt
    14521 tatatttagc gtgcgggttg gagttggggg cttcttctac cagcagaatg tttgcacctg
    14581 cgctgcctgt gcccctcacc ctagccctct ccccaaaggg gcgaggggac cgtcccgtgc
    14641 aatgtttagc tcattttctg aaagggaacg gtggtttccc cctcgcccct ttggggagag
    14701 ggttacagtt cgcacgtaat ccctcccggc aacatttatt gccgcttttg tttacatatt
    14761 ctgccgctaa acaattctcc attcctggcg tatatctggc taacattcat caatgtgata
    14821 gattcctctc ccgcatttat gggaatgcgt agtgacttat tctaattatt tttataaaag
    14881 catccgtgat aatgaaaagg caaagaaacg tcaatttgtt attgatgttg gtattactcg
    14941 tggccgtcgg tcagatggcg caaaccattt atattccagc tattgccgat atggcgcgcg
    15001 atctcaacgt ccgtgaaggg gcggtgcaga gcgtaatggg cgcttatctg ctgacttacg
    15061 gtgtctcaca gctgttttat ggcccgattt ccgaccgcgt gggccgccga ccggtgatcc
    15121 tcgtcggaat gtccattttt atgctggcaa cgctggtcgc ggtcacgacc tccagtttga
    15181 cggtgttgat tgccgccagc gcgatgcagg ggatgggcac cggcgttggc ggcgtaatgg
    15241 cgcgtacttt gccgcgagat ttatatgaac ggacacagtt gcgccatgct aacagcctgt
    15301 taaacatggg gattctcgtc agtccgttgc tcgcaccgct aatcggcggt ctgctggata
    15361 cgatgtggaa ctggcgcgcc tgttatctct ttttgttggt tctttgtgct ggtgtgacct
    15421 tcagtatggc ccgctggatg ccggaaacgc gtccggtcga tgcaccgcgc acgcgcctgc
    15481 ttaccagtta taaaacgctt ttcggtaaca gcggttttaa ctgttatttg ctgatgctga
    15541 ttggcggtct ggccgggatt gccgcctttg aagcctgctc cggcgtgctg atgggcgcgg
    15601 tgttagggct gagcagtatg acggtcagta ttttgtttat tctgccgatt ccggcagcgt
    15661 tttttggcgc atggtttgcc ggacgtccca ataaacgctt ctccacgtta atgtggcagt
    15721 cggttatctg ctgcctgctg gctggcttgc tgatgtggat ccccgactgg tttggcgtga
    15781 tgaatgtctg gacgctgctc gttcccgccg cgctgttctt tttcggtgcc gggatgctgt
    15841 ttccgctggc gaccagcggc gcgatggagc cgttcccctt cctggcgggc acggctggcg
    15901 cgctggtcgg cggtctgcaa aacattggtt ccggcgtgct ggcgtcgctc tctgcgatgt
    15961 tgccgcaaac cggtcagggc agcctggggt tgttgatgac cttaatggga ttgttgatcg
    16021 tgctgtgctg gctgccgctg gcgacgcgga tgtcgcatca ggggcagccc gtttaagcgc
    16081 acgtcaccgc agcatcgtca tcagctccat gggagaacga tgctgcttta tcagatcacg
    16141 catcacccgc atatgcggtg cggagtaaga ataaaacgcc tgatagcccg cacaaagcac
    16201 gctgtctggc gtctcctggc gagcgtgcgg gcacaggcga tgccagtcgc acgcctggca
    16261 gtcacggcgc ggttgcgggt tttcgctgcc acaccatttt tgtaaggtgg cgtcaaaaag
    16321 ctcaatcgag atacgactga tatctgcccg cacccaacac tcgaacaggg caattaaaaa
    16381 tttcccccac agctctgctg tcaccgaagc ggccgtcaga tcgccccggt catcaaattc
    16441 aagataggga atgagctgta cggagcggat gccccgctgt ttgaaattgt gataaaaatc
    16501 ggcaggttgc agagcatcct tgtactctac aaccagttta tgcctgtcct cttcagcatc
    16561 aatgacctga cttcctgtca tgcgactctc tcaataaata cgataagttg atgtatatgc
    16621 gttacagcaa acagtattgc gagagataac accgcgaggg agatcaaaaa tttgtcacgg
    16681 actacgtgaa attgggaaaa atcgctattc gtgacatcca ttaaattgcg attacgggtg
    16741 tagtgccaga ggatcagcgc cacgatggcg agaatgccaa tcgaaatcca gaataacatg
    16801 cccgcctggt gccagttgtg tttgatagcc aacgccatca acgcgccgta gcccagcatg
    16861 gtacgaaacc acgccagtga tgtgcgctcc ggctgcagtc ctgggtcggc aatgcgtctg
    16921 gctttgcggc tatccggcat acaacaccag tcccataacg atcaccgcca cgaccatcaa
    16981 tattaagcta atgattaata agctgttggt gtagggcagg tcctctttca gacgcatcgc
    17041 tttttcattg cgtaaccagc gcaggtagcc atacatcgct aacccaccgg aaaatagaca
    17101 caacaacaac gccagcagtt cgcgaatgac gggcgtggcg aaatctggcg caagttggtc
    17161 aagcccaacc cccgccgcca gaaatcctag tgcggtacgg atccacgcca gaaaagtacg
    17221 ctcattagcc agcgagaagc ggtaatcggg cgcttctccg aggcgggaaa ttttcattgt
    17281 tgctcctttg caatttcact aacgctctat ctttagcggt tttttttaag aatttcgtcc
    17341 gcccgtttcc tgatatgctt ttgccatccc ctacaaggag gtcagatggg tatcatagcg
    17401 caaaataaaa tcagttcatt gggcatgctg ttcggtgcca tcgctttaat gatggggatt
    17461 atccactttt cattcggccc tttttccgca ccaccgccaa cgcttgaaag catcgtggcg
    17521 gacaaaacgg ctgaaattaa aagaggtctt ctcgccggaa taaaaggcga aaaaattact
    17581 accgtggaga aaaaagagga cgtagatgtc gataaaatcc tcgatcaaag cggtatcgca
    17641 ctggccattg ctgcgctact ttgcgcattt attggcggta tgcgtaagga aaatcgctgg
    17701 ggcattcgcg gcgcgctggt ttttggcggc gggacgctgg ctttccatac cttgcttttt
    17761 ggtatcggca tcgtgtgcag cattttgctg atattcctta tcttttcctt cctgactggc
    17821 ggttcactgg tttaagcagc ctgaataatg cccgccgggt gttcatcagc gggcattatc
    17881 gtcagaactt ctgttttttc tcttccactt tcactccttg cgtcggtagg ccggtgtcat
    17941 agtcacgtac caccggcgaa tagccatctt gtgggcgtgg acgaaatgcc cccatccagc
    18001 gcggcagtgc atctttacgc cacggacgca ggctccattg gtaactgcgg aacggatcgc
    18061 gaattttgtc catgtaatcc aataaggcgt catgcatttt gctgcgaacg tctgcaaaac
    18121 ggatatcatc gatcaggtta tgcatttcat ttgggtcatt acggcgatcg taaagttcat
    18181 cactggtgaa gaggttgagt accagtttaa agtcatccgt cacccagcaa cgcaccggaa
    18241 taaaaccgcc aaagctgtca tgctcaatct cgtagcggtt aaattccacc attacaccgc
    18301 gtggctcttt cacggcaagg atattttccc ccggcagaat ctctggtttt tcaatatctg
    18361 ccagcgccat cattgtcggc agtaaatcga tatgactgac tggcgtatcg acctgtcgcc
    18421 gctccccttg cggcgaacgg atgatcagcg gaatacgggt gatgtcgtca tacatcgccg
    18481 ctcctttact gatcagctta tgtgcgccca tcatttcgcc gtgatcggaa gtataaatga
    18541 cccacgtatt ttcacgttgc tctggcgtta aggcattgat gacccgtccg atttggtcat
    18601 caacaaagtc attacaggca aaatagagcg gatggtgata aagcccgtca tcaccgactg
    18661 gcgatggcat cgcctgcgcc cataagcggt gatgttccgg tttgttcgcc aggtcatcct
    18721 gagctttctc gcccagatcg tagtaaaaat cagcgtattt ctctaaatac tccaccggac
    18781 aggtgaacgg gtgatgcggc tcatcatacg aaaccaccat caggaagggt tcctcggcgc
    18841 gcgcgggctg ttgcagaaaa tctaccgccc gattgctgat gcggtgcgcc caggtgaagg
    18901 tttcgtcgat atggttcgcc tgtaaatcct caacgctgtt taggccattg cgccacaggc
    18961 tgatctcttt ttccgtcagt tcgctaaggt agttcgcccc atcgaaccag taatcagcgt
    19021 cccactccgg cggacactcg ccagtgccga aatagtcatg accgtcgaga tgccatttgc
    19081 cgatgtaaca ggtgtgatag ccggcatcct taaagtagcg ccccatagtg gagatgtttt
    19141 tgcctggcgc gacgttgttg gtccacgggc cggactggtt agcgtagata ccggtaaata
    19201 gtccggcgcg tgcaggcgta caaaccggtg aacaggtgta ggcggaatta aagcgaatac
    19261 cttccgccgc cagactatca atattttgcg tattcagcgg tttaccgcta tagcaaccga
    19321 ccatattggt ggcctgggta tcggtcatga tgaacagaaa attggggcgt ttcataggct
    19381 atccttcgcg tcgggagtaa aggttacggc tgggtcgtga cgatgccgcc agctgtcaaa
    19441 aatcaggtag acaatcacta cggcggcaat gaaataactg atcatcgata accagcgcgt
    19501 accgtagccg ccgaattcag ccagcccggc atagacgcca atcatcgcga acaggatgcc
    19561 aattgacgcg atcttgacgt ttctccacgg tttcatgtcc accgcaaacg catctttgaa
    19621 ggtgaacggc gtggagcgcg gtttgataaa accgatcacc agcatcacga ccacgttgat
    19681 gcagaacgta caggccagca catagaggaa atggaagtcg aacttcacca gatagttgat
    19741 ggtgatgtag ctgattatgc caatccccat cgccactttt gccgccagcg ccgggatgcg
    19801 cgggaagaaa aagcccataa tgatgatggt aaccagcggc acgttgtaaa tgccgttgag
    19861 ctgtttcatc cagctataca gcccctgcgg cgcgttggcg atccacggcg cgaccagcac
    19921 cgaaacgata gcgataaaga aaccaaattt gcgcccgacg gtgaccagct gctgcggctc
    19981 ggcattctgg ttaatgatgc gacggtaaat acccatactg aataacgtac tggcgctatt
    20041 cagaaagccg ttgaaggtac tgatcaccgc accaaataac accgcgccga agaaacccac
    20101 cattggcact ggcagaacgt tattgaccag cgtcgggtag gccatgtcgg ctttcggtaa
    20161 atcctgatac agatgaaacg caatcaaccc tggcagcacc agtaccagcg ggtcgagcat
    20221 tttcagcacc gccgttaaca gcgccccctt ttgcccttcc gccaggcttt ttgacgccag
    20281 cgtgcgttgc acgatgccct gatttgtaca ccagtaaaag gtgttcacca gaatcaaacc
    20341 ggtaaatgcc gcgccaatcg gcaagggatc ggtcgggcca ccgattgagt ttaatttctc
    20401 ggcgtgaacg gtggtgagtt gctcaatgcc ctgcataaag ctgcccttgc ccatcgcgat
    20461 caggccaaat accggcacca tcaacccgcc gataaccagc ccaataccgt tgatggagtc
    20521 cgccactgcc attgcgcgca gtccgccgat caccgcatac aaaattcccg ccagaccaag
    20581 caaaattacc agtagccaga tagccgcacc gtgagaaatc tgtagcgatt ccccgacgtg
    20641 aaacaggctg ttgagcgcca acgcgccgga gtagagaaca atcggcagaa agcagacgcc
    20701 ggtggcaatg aggaagcaga agtcgatgat aatacgcgtc gttttatcat aacgttcctc
    20761 cagaaaatcg gggatggtgg caatgccgcg cttcagatag cgcggtaaaa agattagcgc
    20821 gaggaagatc agcgtcaccg ctgaagtcac ttcccagccc atcaccgaca tgccgctttt
    20881 gtaggcctgc ccggaaaggc cgaccagttg ttccgtggaa aggttggtta gcattaacga
    20941 agcggcaata accggcgctt ttagtgaacg tccggcaaga aaatagcctt gttgcgatcc
    21001 ggtatctatt ttgcggacct tccaccaggt gatcaccgcc accagcagcg taaaaccgac
    21061 aaaactcaag atttgtaacg aattcatctc atagccctta tttatgttta tgtcccggac
    21121 gcatgaatat agggagaggt aaaatgcgtc gggtcaggca gtcagattct taaaatacaa
    21181 acgtcgtatc cctgaacgga ttgttttctg ttaagttcag gttgtgagca taattcgccg
    21241 gagggatatt aaaatgaatg gaaaattgca aagttcagat gtaaaaaacg aaactccgta
    21301 caatattccc ttattgatta acgaaaatgt gatctccagc ggaatttctc tgatctcgct
    21361 ctggcatacc tacgccgacg agcattaccg ggtgatctgg ccgcgggaca agaagaaacc
    21421 gcttatcgcc aactcatggg ttgcggtgta taccgtacaa ggatgtggga aaattctttt
    21481 aaagaatggc gaacaaataa cactgcatgg caactgtatt atattcttaa agccaatgga
    21541 tattcactct tatcactgtg aaggtttagt ctgggaacag tactggatgg aatttacccc
    21601 caccagtatg atggatattc ccgttggtca gcaaagcgtt atttataatg gcgaaattta
    21661 taatcaggaa ctcaccgaag ttgctgagtt aataacttca ccagaagcaa taaaaaataa
    21721 tctggcagtc gcttttctga cgaaaattat ttatcagtgg atttgtctta tgtacgcaga
    21781 tggtaaaaaa gatccacaac ggcggcaaat tgaaaaatta attgccactt tacatgccag
    21841 tctgcaacaa cgctggagcg tagctgatat ggctgccacg atcccctgta gcgaagcctg
    21901 gttgcgtcgt ctgtttttac gctataccgg caagacgccg aaagaatatt acctcgatgc
    21961 gcgtctggat ctggcgctat cgctattaaa gcaacaagga aactcggttg gcgaagtcgc
    22021 tgatacgctc aacttcttcg actcctttca tttcagcaaa gcctttaaac ataaatttgg
    22081 ttatgcgccg tcagccgtgc taaagaatac ggaccagcac ccaacggatg ccagtccaca
    22141 caattaacgt aactctggcc agtacgcttt gttggcctcc agcaactcat ccagaatagc
    22201 tttggcgacc gaggcgctcg gtaccgtttt cgacaacgta atcgcctacc acaggtgctg
    22261 atatgaacgc tgttcccagg cgtccacgac cagtttttcc accgccactt gctgactcat
    22321 taaccctttc tgaaactgcg ggatatcgcc gaccaccagt ggttcaggcc gtagtagcgc
    22381 acgcgcatct gtttgcgatc ttgcagccca acaatttgcg ccatccggct ttcaataccg
    22441 attggcatgt cacagatgtt gaggattttc gcattcgggc gcagacgacg cgtggcttct
    22501 gcgacaattg ctgccgggtt ggagtagttg agcatccagg cgtttggtga atatttttcc
    22561 atataatcca ccagttccag gacgccgcca atggaacgca tgccgtacgc tattccgccg
    22621 ggtccgcaag tttcctgacc aacaacaccg tggcgcagtg ggattttttc atccagttcg
    22681 cgcatcgggt acttgccgac gcggatgtgc gccatgacaa aatcaacgtc gctgaatgcc
    22741 acttcaggat cggtggtgta actaaacgca atgtccggcg ctttctcttt aaggatgact
    22801 ttacaggctt cggcaatcac ttcctgccgc gcaccatcgt tatcataaaa tttcagtgca
    22861 cgaagcggga aacggtcctg attcgccagg agcatcaaca cgatgcctgg cgtaaaggtg
    22921 cttccaccgc ctgcgacaac cactgagaat ttggtcatta tactgcctcc gtaatggcaa
    22981 cattttctgc tgattgatga gaattaatta agctatcgag ctgttcacgc agctgggata
    23041 catgcagacc aatgatcacc tgaatggcat cgccactacg gaagacgccg tgcgctccca
    23101 gctttttaaa gacttcgtca tccagcgttt gtgacatgtc atgcagtgca atacgtaaac
    23161 gcgtcgcgca attgttaatg ctggagatat tgccgacccc gcccagggct tgcaggatac
    23221 cggcagcctg atccagctct ttttttggct ctgccgcggt ggtttggcct cgcgaggctt
    23281 tgtattcggc ttttgagtag agtttcactt ccgcatcttc acgtcccggc gtgcacatgt
    23341 tgaactgcag aatcagtgtg cggaaaacca cgaagtacag cagggtaaag cacaacccaa
    23401 tggcgatttg ggtcagcatc atatccgcgt ggttgctgaa catcgggatc cagttttgcg
    23461 gtaaaacctg gtcaatcaga cctccgccca tgttgcccac cacaccaaag agatacatta
    23521 cggtcgacat tgaggccgcc agcacggcgt gtaccgcaaa cagcaacggt gaaatgaaca
    23581 ggaaggtaaa ttccagcggt tcggtaattc ccaccagcat ggcggttaag gttgcgggaa
    23641 tcagcaagcc cgccactttt acccgatttt ccggtgcggc agtgaagtac atcgctaaag
    23701 aaatgcccac ggcaccaaag atttttgagt taccgtgcag ggcaaaaccg ccttccggga
    23761 acaacgattt cagcggctcg gcgctcaaac tgaactcttg cagatgctgc gcccagtaca
    23821 tctgaatgcc gccttcaaca gctgccggac caaagataaa ctgtccgtag atgaagtggt
    23881 gtaaaccggt tgggatcaga atacgttcga ggaaggtgta aacccagacc ccaagtgcac
    23941 ccgccgaacg caggaacgct tgcagagatt caatccccat ttgtactttt ggccagccga
    24001 gcaacgtcag ccaggcacag gggatcatca ccaggaaggc gataatcacc acataagacg
    24061 tcccctggaa aatgccgaga aaaaccggca gttttttatc gaacagacgg ttatgcagcg
    24121 ccgtcacaat gccggaaatg ataattgcgc cgataatgct ggtatcgagg gttttaatcc
    24181 cagccatcat tgtcagaccg ctacctgcca ccgcgtcctg agtgaaatcg acgccgaagt
    24241 agcttcccca ggtcattccc atcgcattga tgaaatagtt ccaggtcagg aaactcacca
    24301 tcaccgccag acaagcacgt ccctgcgctt gcttagcaag gccaatgggt aaaccgacag
    24361 caaaaatcag cggcatatta cggaataccg tccaaccgcc ctcttcaata atgtgtacga
    24421 tttgcgcgaa taaactgttc ggatcggtca gtgattcccc gacaaacatc gggttttgca
    24481 gcaagatggc aagacccacc acaatcccgg cgaagggaaa cagcagcact ggcgtgaaca
    24541 tcgcgccgcc aaagcgttga atttgactga gcatttccac atcctctttt ctcaattctg
    24601 taagtaggaa gcctgattgg ttatgcggca ggcctggagc gaggatagga agatgcgatc
    24661 ggcagaacat gacgacccaa tgggattcgt gatcgctttc atgcttttcg ttgggcggta
    24721 gagatctacg ttttcgcgaa aaagtagaca tctgtgagca ctggtacaca gcatcgaaca
    24781 catctttaaa aaaaagatgt tttttcaatc gattaagcag aacttgtggg cgcattaccc
    24841 gggcttgcag gcaaaaaaga gatctagaga tgatctacaa aagcattgcg gagcggttaa
    24901 gaattcgact taattacgcg gatttcacgc taaacagcct tcttcccggt gaaaaaaagt
    24961 tggcggaaga atttgcagta tcccggatga ccatccgtaa agcgattgac ctgctggtag
    25021 cgtgggggct ggtggtccgc cgccacggca gcggcactta cctggtgcgc aaagatgtgc
    25081 tgcatcaaac cgccagcctg accggactgg tggaggtgtt aaaacggcag ggaaaaacgg
    25141 tcaccagcca ggtgctgatt tttgaaatca tgcctgcgcc tccggccatt gccagccagt
    25201 tacggattca aatcaacgag cagatctact tctcccgtcg cgttcgtttt gtggaaggga
    25261 aaccgctgat gctggaagac agctatatgc cggttaaact gttccgtaat ctttcgctgc
    25321 aacatctgga agggtcgaag tttgaatata ttggacaaga gtgcgggatt ttgattggcg
    25381 gtaattatga aagcctgacg ccggtgctcg ccgatagact gctggcgcgg caaatgaagg
    25441 tagcggaaca cacgccactg ctgcggatca cctcgttgtc atatagcgag agcggggagt
    25501 ttttgaatta ttcagtgatg ttcagaaatg ccagcgaata ccaggtggag taccatttac
    25561 ggcgactcca cccggaaaag agttaaccga tactccagaa gagcaccgcc agtaattggg
    25621 gggtgataat gcgcagaaac atcactaacg gatagacagt ggcgtaagag agcgccgccg
    25681 caccactggt tggatgaaga ttattagcaa acgccagcgc cggaggatcg gtcatggaac
    25741 ctgccagcat cccgcacatg gtcaggtaat tcattttggc taacatccgc gccagaatgc
    25801 caacagtaat cagcggaacg gcggtgatca gggcaccata accaatccag cttagccctt
    25861 cgccattgac cagggtattc acaaaatccc caccagattt cagaccaacg accgagagga
    25921 acagcacgat ccccagctcc cgcagcgcga ggttggcact tggcggcata aaccagtaca
    25981 gcttgccgat actgccgata cgcccgagga tcaacgccat aatcagcgga ccgcccgcca
    26041 gccccagttt caacgcggcc gggaacccag gcacaaagac gggaatagaa cctaacaata
    26101 cgccaagccc gatgccaata aacactggca gcatctgaac ctgttgcagt ttttgctgcg
    26161 cattccccag cacattggca acggcatcaa ttgcggacgg acgccccacc agattgagga
    26221 tatcgccgaa ctgcaggctg atatcgccac tggcgaccag ttcgaccccg gcacggttca
    26281 ggcgcgagat aacaacgtca tagcgttctt taaagtgcag gtcgcgaata cgttttccga
    26341 gcacgttttc attggtgacc accacacgct cgacgcgcaa atcagtgcct ttcgtggaca
    26401 gcgaagtatc gacctcctga ccaatcacca gttgcgcatt atgtaaatcc gctggctgac
    26461 ccaccagatg cagcaaatcg cccagttgaa taatggtatc tggcgaagga acttttaggg
    26521 tttcttcgcg tttcagacgc gagcagataa ttttgtcgcc gttgagaatc ggtacatctt
    26581 taatggctaa atcatgcagg ttagggttct caacgcgaat attgatagtc ttgatcagcg
    26641 cgccgccatt ggtgcgtgaa gactcgtgct gctgagcttc tgtctcgaca ttgacgcgga
    26701 aaataacccg caacatccac atggtgaaca aaatcccgca aatgccgaat ggatacgcca
    26761 tcgcgtaact catccccatc tgatcgacca tttccattgg tgtacccagg tcgcgcaaaa
    26821 tctgctgccc tgcccccagc gctggcgtat tggtaaccgc accggagaaa atccccagca
    26881 ctaccggcag tggaatatca aacagtttat gcaggatggc ggtaaccaga ccaccgatga
    26941 tgacgatcag aacagcaaac aggttgaggc gtaatccgga gacgcgcaat gaggcaaaga
    27001 agcccggccc tacctgaatc ccgatagtat aaacgaacag gatcaggcca aattcctgaa
    27061 taacatgcag catatcgcta cttaatgtca tccccgcctg agaaacaaaa tggccgacga
    27121 tgatcccacc aaacagcacg ccgccaatac ctaatcctat gccgcgaaat ttgacattgc
    27181 cgataaacaa accgacgact gccaccaaag ccagaatact gaccgttaat gctatatcac
    27241 tcatcgtcca tttctcttga gataacattt ttaattgcta cggattatgg ctgaatcggt
    27301 gaagcagatt tgccttatag cgcacattat cacgggaaag aacgtgccga aatatcttaa
    27361 acagtcgaaa tttaacgcat tgttgagtaa gtaagacaaa aaaagcccca ccagaatggc
    27421 ggggcaaaga gaatagctag ttagctattt aacgcgggac gttcgctgat agcgatacgc
    27481 tgcgctgcga tgggttcagg ctcattacga attaaatcaa tatgcagtaa accgtttacg
    27541 aaggttgcgc cagagacttc catattttca gccagcgtaa agctcaggct aaatggctga
    27601 ttcataagcc cttgatgcag ccattttttc tcttcttttg gctgctccgg cgtgcctttt
    27661 acgctcaggc gcgtaccttc cagttgaatc tctaaatctt cctgacggaa acctgccagc
    27721 gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc
    27781 tggctttcac cggcgttttg cagtgcgttg gccagtttgt caaaaccgat ccattgacgc
    27841 atcagtgggg ataaatcgaa gttacgcata gtcatttctc cttctaagaa gcgagtaagt
    27901 acctgcaaat ccgaagattc gcatatgctc cctgacggcg agcatggaga tgtcaggccg
    27961 cgccaggcgg ccttagggaa ttagttgatt tcgatacggc gcggtttttt cgcttccgga
    28021 atcacgcgtt cgagatcgat atacagcaaa ccatttacca ggttagcacc acgaacatga
    28081 atgttctcag ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga
    28141 taggtgcgct ctttttgttc gtcggcgtga gcacctttca ccaccagcag attatcctgg
    28201 gcggtaattt ccagttcgct ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg
    28261 ttttcgtcta ccagttcaac gttatacgga gggtagccgc cattactctg gctctggttg
    28321 ttttctaagt ggttaaacaa acggtcaaat ccaatagcag aacggtaaag cggggataaa
    28381 tcaaagttac gcataatcaa tagctcctga aatcagcgag aatgtaagac cttccacaat
    28441 ggacaggtca ggtagccaga acacccatca ggcgcgtcct catcggctac gatgtaaaaa
    28501 tgggtctgga aatgaacttt tcaagcctta tcagcggact tttttttgca gtttatggtc
    28561 tattgcatag actgaggggg cagcacgaaa cgttaaagtg cgatagccgc cacagtgcga
    28621 cgtaatgatg atgttatttt cagcaaggga tcgctataac tcatcaggcc aacacccacg
    28681 atcaacagat gaatttatta tgataagaaa tgtgttgtta gcgttcatga tatgcagcgg
    28741 aatgacatta ctcggaggat gctccagcgt gatgtcccat accggaggga aagaaggaac
    28801 gtatcccggc acccgcgcca gcgcaaccat gattggcgac gatgaaacca actggggcac
    28861 taagtcgctg gcgattctcg atatgccgtt taccgctgtg atggacacgc ttttactgcc
    28921 gtgggatgtg ttccgcaaag acagttcggt gcgctcacgc gttgaaaaaa gcgaagccaa
    28981 cgcgcaagcc accaatgcgg ttattccacc ggccagaatg cctgataact gatttagcga
    29041 tcagtttcgg tgatccacag ctggccacct tccatccacg ccagccattg accatccggc
    29101 gagaagacca cggcgtccgc agaaggtgga ttggcgtggt tttcggttaa atactcaacc
    29161 tcgccgcttt gcgcatgggc acaagcaatt cgattatcca gcacaaagcc caaccattct
    29221 cctgacggat gccagttaaa tgcagactga atatccgttt tgttatgggt taactggcgc
    29281 ggctcgccgc cctgtggcga gataagccac agttgcacaa tgccgttatc atcacgcatt
    29341 aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg gacgttgact
    29401 aaccccggat aagcccgatg gtgggtaaag gttaaacgtc gctgcacgac gccgcgcggt
    29461 ggcgcgggca gggtggtttc cgttccactt aacggcgcat cacctgccgc tttccagcca
    29521 gcttcatctt gcggtaactc aacgataaac agctccggca ctttctcgcc ctttggcgaa
    29581 agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc atccttcttc ataagcacga
    29641 ttgatttcat cgctgccagg ctgcggcgtg ggcgtggttt tactcaccag cacgcaccag
    29701 tggctaccgc tgtattcacg cggatgctgc ttttgtacgt tgaccgggcc aaacggcgca
    29761 gcaacaccga cgtttcgcaa atccagcgcc ggatcgagtt catgcattac atggtcgtta
    29821 taggtaaagc tcaccctttc accgttcggg ctaaagacat gcacatggct gccgccgcgc
    29881 agcgctcctg gcgtatacgg cgcggtaata tccattgcat cgagattgct caccttgccg
    29941 ccttcagcaa tgactccgcg tcgatgatgg aaatcgtaat gccatgtttc atcaggattt
    30001 tccgggccat ggatgaaaac atatttctct gacttcggat gaacggtcac cacgccgacg
    30061 tgtgcgccct gtgacgcgcg atagataacc tcgacctcgc cggtatggat attcacacgc
    30121 tcaatggtct cgccggtaaa cgacgcccca gaaggacgca cgtcaaatac cagccactgg
    30181 ctgtcgggcg tccaggtatt ggtattggtg agcaggtgat tacggggagc aaaggttatc
    30241 tgtttcatgg cgcggtcctg atacaaaacg ggtcccacca tcatacttca caaggacttc
    30301 actttcagac aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat ggaacatttc
    30361 gacgtggcga ttattggcct cggcccggca ggatcggcgt tggcgcgaaa attagcaggc
    30421 aaaatgcagg tgatcgcgct ggataaaaag caccagtgtg gtactgaagg tttcagcaaa
    30481 ccttgcggcg gtctgctggc accggacgcg cagcgatctt ttattcgcga tggactgacg
    30541 cttcctgtcg atgtgatcgc caatccacag attttcagcg tcaaaactgt cgacgtcgcc
    30601 gcatcgctca cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca tgctttcgac
    30661 ttgtggatga aatcgctgat ccccaccagc gttgaggttt atcacgatag cctgtgccga
    30721 aaaatctggc gtgaggatga taaatggcat gtcatttttc gtgcagacgg ctgggagcaa
    30781 catattaccg cccgctatct ggtcggtgcc gatggcgcaa actcgatggt gcggcgacat
    30841 ctctacccgg atcatcaaat ccgtaaatat gtcgctatcc agcagtggtt cgcggagaaa
    30901 catccggtgc cgttctactc ctgcatcttt gataattcga taactaactg ttattcatgg
    30961 agtatcagca aagacggtta ttttatcttt ggcggtgctt atccaatgaa agacggtcag
    31021 acgcgtttca cgacgctgaa agagaaaatg agcgcctttc agttccagtt tggtaaggcg
    31081 gtgaaaagcg aaaaatgcac ggtgctgttt ccctcacgct ggcaggattt tgtctgcggt
    31141 aaggacaacg cctttctgat tggcgaagcg gcaggattta tcagcgccag ttcgctggag
    31201 gggattagct atgcgctgga tagcgcagac attctgcgtg cggtgttact gaagcagcca
    31261 gagaagctca ataccgctta ctggcgcgcc acccgcaaac tgcgtttaaa actcttcggc
    31321 aagatagtaa aaagccgatg cctgaccgca ccggctttaa gaaagtggat tatgcgcagt
    31381 ggtatggcgc atattccaca gttgaaagat tagccaacgc gcttcacatc gcccaccagc
    31441 aggatgtaag agagcgcgcc aatcaacgcg acggcggaga tataaaccag tgcaggtgcg
    31501 aaaccgtaac cctgcgccag gtaccccacc accagcggaa cggtgatgcc gcccagacca
    31561 ccggcgaagt taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc cagagaagag
    31621 accagcgacc aggtaatcga agcaaaaccg ttgccgaaga atgccagcgc catcaggcac
    31681 ataatcatca tcggatcgtt ggtgtagtta gcgcccataa tgcaggtgga gatcagcaag
    31741 ccgcagataa tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg taccagcaga
    31801 tccgcgaccc agccagagag caggacgccg acaaacgccg cgaggaatgg caccgtggtc
    31861 ataaagcctg ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt cgggaaccag
    31921 gttaagaaaa accacagtgt agaagccacc gcaaattgcc caagataaac gccgatcagt
    31981 ttacgatgga acaccagttt ccagtctttg gctgttaacg gctgacgcgc ctctttcttc
    32041 accggcgcat caccatccac cagaccgccg ccatcacgaa tgtaatccag ttcagctttg
    32101 ctgatacctt tggtcaggcg cggcggctga taaaccttaa accaaatcag cgaccagata
    32161 atgccgatac caccagtgac aatgaacacc cagtgccagc tcaacatctc ctgaatccag
    32221 atcagcagcg gagtcaaaaa cgccagaccg acaaactgac cagacgtata aaaaccaacg
    32281 gcggaagcgc gttcatgttc cgggaaccag ctggtcacca tccggttatt ggtcgggaaa
    32341 gcgggcgctt cgaaaatacc ggttatcgca cgcagaccaa ttaatgacat taatcccgtg
    32401 gcaaagccct ggaataaagt cgccactgac caaccaaata tcgcaataaa ataagtcacg
    32461 cgatctaaaa accaaccgcc ggggatctgg catagcgtat aaagccaggc gaaggccgaa
    32521 aatacatagc ccatttgcgc tttggtaata ccgaactctt cctgaatatg ggcggaagcc
    32581 acggccaggt tagcgcggtc aacataacaa atgaccaccg taataaagat catcaccagc
    32641 gtcagataac gccgacgccc cggttttgct gcattaacgg gaatatccat agcgagcttt
    32701 ctccagattt tgggcatagc gaagccgctc accatgccct gtaatttaca gagggttata
    32761 tttttgtatt gctgttttag tgcccgatga ggggcttacg tggcaggaat taccactctg
    32821 ctacgctgtt atcttcatga cgccagagcg gattacgcca gtccggggca tttttactga
    32881 actcaataac tttagcttcg tcgatttcca cgcctaagcc cggtttcgtt agcggtttaa
    32941 agaagccgcc aaccatgctg aagtcttctt tgtttttcac aaagtcgagt aactccgcgc
    33001 ctttgttgta atgaatgccc atactttgtt cctgaagtac ggcgttatag gaaacaaagt
    33061 cgatatgcag gcaagccgcc agtgcaatcg gtccgagcgg acagtgcggc gcaagggtca
    33121 catcataggc ttctgccatt ccggcaattt tgtagcattc ggtaataccg cctgcatggg
    33181 agaggtccgg ttgcagaatc gaaataccac ctgcctccag cacgcgttta aaatcgaaac
    33241 gtgagaacat gcgctcaccc gccgccagtg gaatatgcgt ttgtgccgcc agtttcgggt
    33301 agtattcggc ttgttccgcc agcaccggct cttcaataaa cagcgggcga tacggctcca
    33361 gttctttaat cagcactttc gccattggcg cgctgacgcg accgtggaaa tcaagaccaa
    33421 actcaatctg attgccaaaa gcttcacgaa tttgtgccac ggtgttgact gccgcatcta
    33481 ccgcgcggga gttatcaatt agccccagtt cttcacaacc gttcagttta aaggtatcga
    33541 agccgatttc gcgcagggtt ttaatgccgt cgataacatc cgccggacga tcgccgccga
    33601 cccaactgta ggctttaatt ttgtcgcgaa ccaggccgcc catcagttgc cagaccggcg
    33661 cattcagcac tttacctttg atatcccata acgcctggtc gatcccggcg atggcgctca
    33721 tcaggattgg accgccacga taaaatccgg cgcgatacat cacttgccat aagtcattga
    33781 tgcgcgatgg atcctgacca atcaaatagt cacccagctc gtgaactgcc gcttccaccg
    33841 tgcgggcgcg gccttcaatc acgggctcgc cccaaccgac cacgccttca tcggtttcaa
    33901 ttttcaggaa catccagcgg ggaggtaaac gatacgtggt aattttggtg attttcattg
    33961 cactgcctct cgatacgcct taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga
    34021 ctgcccggcg cgatagagat cgctgcctaa gcccgcccca gcacaacctg cgtctatcca
    34081 ctgcgccagg ttttctggcg tcacgccgcc aacggcaaag actgcgatgt ccgatggcaa
    34141 taccgctttt aacgctttga tgtattgcgg accaaaagcc gatgacggaa atattttcag
    34201 cgcctgcgcg cccgcttcga gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca
    34261 gacggtcatg ccgtagccca ccgcacggcg gatcacttca ctatggatat tgggcgtaac
    34321 gatgagctga cagcccatcc tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc
    34381 tgcgccaatc aacgccttgt cgccatacgc atcaacgatg gcgggaatgc tttgctccca
    34441 ttgtggggaa ttcagcggga tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac
    34501 atgcgccagc gcctcgtcgg gcttaatgcc gcgcaaaatg gcgatcagcg gaagtttagt
    34561 ttgccactgc atgagcgatg ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg
    34621 ccgtcacgtc gcaacccatc gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc
    34681 cggcgacaag ggtgatggcg tgttgatggg tcacatagtc gcgcatactg gcaacctctg
    34741 cgccaatcaa caaaccagag agaaattcgc tgacctgttc gcggggaagt gttcccagca
    34801 catgcgaggc gcgaacttca aaaagctgcg gcaatatggc gggcgcatta aggccgcgct
    34861 caaggccagc cgcgaaggca tcggcagagt tttcctgcgg cggcaaacct gcgccaatca
    34921 atgagtgatt taacagtaaa tgatgtaatt caccggtcat cacggtgcga aaatcgttga
    34981 tttgctggct atcggcctgc acccatttgc aatgcgtacc gggcataaca taaagagagg
    35041 aaggagtcag agctcgcgcg ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt
    35101 tatcgtcatg agagacgcat aatccgggaa taatccagat attgtcgcca acagacgtta
    35161 attgttcgcc aatagacgaa aaacgggcag gaacagataa atacggagca actttccagc
    35221 cgacattgct gccgaccatt cctgccatca ccactggcgt attttcttca cgccagtcgg
    35281 tcgtgacttc tgctaacacc gcagccggag attttccgtt caggcgcgtg acgcctgctt
    35341 ctgattgcct gctctccagg cagtggtcgc cctgataaag ccaggcgcgc agattggtcg
    35401 atccccagtc aattgcgatg tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga
    35461 gctggcgatc atggtaagcg ccgcctgctc tgccgcatcg ccgtcctgat gccgtattgc
    35521 atcgaacagc gccttatgtt cctggagcgt ttgcggcatg ttggcctcat cgcccatcca
    35581 ggttcgttca aaaaccgccc gctgcagcga actgatcgca atgctaagtt gctgtaacac
    35641 cgggttatgc accgactgca gcaccgcctc gtggtagcga atatccgctt cgttaaacgc
    35701 ttcgcggtcc tgattgttgg caatcatctc gttcagcgcc gattcaatct gcgccagatc
    35761 gctggaagtc gcgcgctctg ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac
    35821 ttcgctcatg gcactgataa gccgtgggtc gtagtcgttt tccaacaccc attgcagtac
    35881 gtcagtgtcg aggtaattcc actggttacg cggtgccaca aacgccccgc gataacgttt
    35941 catttcaatc agccgcttcg ccatcagcga acggaacacc tcacggatga tgttgcgcga
    36001 ggttgcaaac tcttcacaga gctccgcctc agccggaagt ggcgagccag gtacgtattt
    36061 gccgtgaacg atctgtttac ccagcgtaat gacaatgcga tcggttttat tgagagtcat
    36121 ggagagtcct tgtgctctgt atgttcttct ctactttacc ccgatcgatg cataacgcgg
    36181 caactttgta gtaccagcgt gatgacgttc gcgtttgccg tgtgagtaat gtagtacaaa
    36241 cttatattgt tgtactacaa tttagatcac aaaaagaaca atgcataaaa aaatgacatg
    36301 cgtcgggcag aaatctgaaa agggatatca ggcgctaaac aggagggaaa gaagagtatg
    36361 ctttcaacgg cttagctact cgtttaaagg attaatcatg aagttgaatt ttaagggatt
    36421 ttttaaggct gccggtttat tcccactggc gctgatgctt tcaggctgta tctcgtatgc
    36481 tctggtttcc cataccgcaa agggtagttc aggaaagtat caatcgcagt cagacaccat
    36541 cactgggcta tcgcaggcaa aagatagtaa tggaacaaaa ggctatgttt ttgtagggga
    36601 atcgctggat taccttatca ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc
    36661 agcacttaac cggcacaata ttcaggttgc cgatgacgca agatttgttt taaatgcggg
    36721 gaaaaagaaa tttaccggca caatatcgct ttactactac tggaataacg aagaagaaaa
    36781 ggcactggca acgcattatg gttttgcctg tggtgttcaa cactgtacca ggtcactgga
    36841 aaacctaaaa ggcacaatcc atgagaaaaa taaaaacatg gattactcaa aggtgatggc
    36901 gttctaccat ccatttaaag tgcgatttta tgaatactat tcacccagag gcattccgga
    36961 tggtgtttcc gcagcattac tgccagtgac tgttacgctg gacatcatta ctgcaccgct
    37021 gcaatttctg gttgtatatg cagtaaacca ataatcagta agcgggcaaa cgcgtttatg
    37081 ctgtttgccc gtccactgat taattcagca catacttctc aatagcaaac gccacgccat
    37141 cttcaaggtt ggatttggtg acaaagttcg ccacttcttt caccgaagga atagcgttat
    37201 ccatcgccac accgacgcct gcatattcaa tcattgcgat atcgttttcc tgatcgccaa
    37261 tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc acccccgtac
    37321 ctttgttaac gcgtttatcg aggatttcga ggaagtacgg cgcacttttc agcacggtat
    37381 atttctcttt cacttcctgc ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat
    37441 caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct tcgcagaaca
    37501 ccagcggaat ggtggcaacg aaggattcat gcaccgtgta gtagctgata tcacggttgg
    37561 cggtgtacag cgtggtgcgg tccagggcgt ggaaatgaga accgacttcg cgagagagtt
    37621 tttccaggaa acgatagtcg tcatagctga gagcagtttg cgccacggtg ctaccatcag
    37681 cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc tgttccatat
    37741 gcagctcttt caggtagttg tgcacacctg catacgggcg acccgtcgtt agcacgacat
    37801 tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
    37861 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
    37921 caggtagatt agttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
    37981 cgagggaaaa gggggatgca acgcgcgttg cgtgctcccc atttgcttag cggaagagtt
    38041 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt ggagacactt
    38101 caccttgcgg tgagagcgca tcaataatct tcggcaattg ttctgccagt aaactggaag
    38161 ctgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt gtgccgagcg
    38221 ccgattccag ttgctcgcca ctaaccgatt gattgccctg ttgattactc agccaggttg
    38281 agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga atgccgccct
    38341 gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc agaaaggcac
    38401 cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgacccaa
    38461 aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg cggagatgat
    38521 ctccgccctt ttcttatagc ttcttgccag atgcggcgct aacgccttac ccggcctaca
    38581 aaatcatgaa aattcaatac attgcaagat tctcgtaggc ctgataagcg tagcgcatca
    38641 ggcacgctcg catggttaac gccattaaat atcgatattc gccgctttca gggcgttctc
    38701 ttcaataaac gcacggcgcg gttcaacggc gtcgcccatc agcgtggtga acagctggtc
    38761 agcagcaatc gcatccttaa cggtaacgcg cagcatacga cgactttccg ggtccatggt
    38821 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    38881 gccgcgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
    38941 acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca gtttctcacc
    39001 cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca gcggatagtc
    39061 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gctcggcatt
    39121 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    39181 cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcggctt ccgtcagcgt
    39241 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    39301 cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc cagccaatgc
    39361 cggtgcactg gcgttggtgt gcagcgttgc gccatccagc gcgatggaga tttggtactg
    39421 atccatcgct tcgtcgtctt taatatactg ttcctgcttg cctttcttca ctttgtacag
    39481 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    39541 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    39601 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacagccaag
    39661 cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc
    39721 aacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    39781 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc
    39841 gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg cacctttacg
    39901 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
    39961 gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca gcagttcgtt
    40021 catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    40081 ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt cacgcgcatc
    40141 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcatt
    40201 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    40261 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcaacgccac
    40321 ttcgacgcca ataccgtctt tttcggtgga gaagtagaag atattcgggt ggatcggcgt
    40381 tttgttcttg ttcagatatt caacgaacgc cttgatgcca ccttcatagt ggaagtggtc
    40441 ttctttgccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    40501 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    40561 gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    40621 cggggcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctggat
    40681 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    40741 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    40801 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
    40861 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    40921 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    40981 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    41041 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    41101 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    41161 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
    41221 ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    41281 tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc gccagacgta
    41341 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    41401 cggcgcgaat gcgtaagtcc gctttgtgcg ggccatgcgc ggtgtaggtt agctggcgat
    41461 cgcgttcaaa attacgttcc agtacctcag catattctgt ctctttctcc cagccgcgct
    41521 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca gccatatcgg
    41581 ccgcgatacc ggcgctgtac tgggcgcgcc aggtgctgat ttgctccgcc agcgggatca
    41641 gttctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc
    41701 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
    41761 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    41821 cttctggcgt tatcagctgc attggcatca ggtgcgccag ttccgcgact ttatgcccgt
    41881 cagtaccgtc gatgcggact ttgctgtcgc cctgtttgtc cttggttaag ccaatcgccg
    41941 tctcgcgctc ttcgccctgt aatcgcccat ggagaacaaa tgcctcctgc tcatggcgaa
    42001 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    42061 ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
    42121 aagcgagatc cgcggtttca atgttgcgga aatcgcggat caacaagcgg gtgagggaca
    42181 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    42241 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag
    42301 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    42361 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcggtgattt tcagctggtt
    42421 ttcgctgaca tacaggcgca cgccgcggaa tttctcgtta gagagaattg ccgcacgggc
    42481 aaacgcctgc ttgagcagat cgcagccagc ttccagatgt ttgtccggat tcttcggcag
    42541 aacgcggcgg taatccggga agcgaccatc caccagtttg gaggtgaaga taaagtcgcc
    42601 aacgtgggcg cgaatattgt tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc
    42661 gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat ggcttggcaa
    42721 agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg ccacggtgcg
    42781 cagttcttca ccttcggttt caaacagcat accatttaag taatagcgaa cgtcctgatg
    42841 cgccatcgaa aactgggtcg cttcaatcag acgcttcatc gttgcctgcg gcagggtaaa
    42901 ttcgacttca ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag
    42961 cgaaaaacgg ctacgcccgg agcgtaccag catccgttca ccttccagct gcacggcaat
    43021 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    43081 cgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc accatttcca tctcgagatc
    43141 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    43201 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
    43261 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat
    43321 cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca
    43381 ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc cggcagactg tggttagtca
    43441 gctctttcgc cagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
    43501 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    43561 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    43621 cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc agcgcccctt
    43681 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcgcccggca
    43741 aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacacgggtt tccagctctg
    43801 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
    43861 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
    43921 gggcgttgaa ggtatggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    43981 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    44041 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    44101 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    44161 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca
    44221 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    44281 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc
    44341 aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    44401 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    44461 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    44521 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    44581 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    44641 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    44701 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    44761 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    44821 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    44881 ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    44941 ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    45001 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
    45061 gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc gtacattttc
    45121 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
    45181 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    45241 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    45301 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    45361 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    45421 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    45481 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    45541 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    45601 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
    45661 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    45721 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    45781 gccgccactg tcacctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    45841 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    45901 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    45961 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    46021 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    46081 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    46141 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    46201 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    46261 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    46321 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    46381 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
    46441 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    46501 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    46561 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctccac ctttggtcag
    46621 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    46681 cacaccttcc gcggcgcggc gtactccacg cctgacgaga agtacgagaa atacaagttc
    46741 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcgatg
    46801 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
    46861 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
    46921 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    46981 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    47041 atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    47101 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    47161 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
    47221 cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta caaagctgag
    47281 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
    47341 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    47401 cacgacctgt cggcacagga cccgtactac atcttgccga tcctgatggg cgtaacgatg
    47461 ttcttcattc agaagatgtc gccgaccaca gtgaccgacc cgatgcagca gaagatcatg
    47521 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
    47581 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
    47641 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    47701 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca
    47761 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    47821 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    47881 gttgccgaaa ccgtgctggg taaactacct aagccgcgct acgccgatta tcttccgttt
    47941 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    48001 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    48061 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
    48121 tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc gattgccgat
    48181 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    48241 ttctccgcac gggtcaacca tctggtggaa gccctcaccc acttgcgcat ttacgtcgaa
    48301 gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    48361 cagcttaatg acgtgattgc cgaactcgat gcagtgcgtg ctgaagcacg tcagggtagt
    48421 ttgttgcgcg aagggatgaa ggtggtgatt gccggacgtc ctaacgccgg taaatcgagc
    48481 ctgttaaacg cgctggcggg ccgtgaagca gcaatcgtaa ctgatatcgc cggaaccaca
    48541 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgatacc
    48601 gctgggctac gtgaagccag tgacgaagtg gaacgtattg gtatcgagcg cgcgtggcag
    48661 gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacaac agacgccgtg
    48721 gatccggcag agatctggcc ggaatttatt gcccgtctgc cagcgaaact gccgatcacc
    48781 gtggtgcgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
    48841 cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat
    48901 ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc gcgtcgtcgc
    48961 cacctacagg cgctggaaca ggcagcggaa catctacaac agggcaaagc gcaactgttg
    49021 ggagcctggg caggtgaact gctggcggaa gagttgcgtc tggcacagca gaacttaagc
    49081 gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc cagcttctgt
    49141 attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca
    49201 ttgttagcac tcctgttatt cctcaaccct ttttttaaac attaaaattc ttacgtaatt
    49261 tataatctta aaaaaaagca tttaatattg ctccccgaac gattgtgatt cgattcacat
    49321 ttaaacaatt ttagaataga caaaattctt gagtattata ctgtagcctc gtgtctttcg
    49381 aggataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg ttcaatatcg
    49441 acaataagat tgtcgatcac cgcccttaat ttacccttct gtagccatca ccagagccaa
    49501 accgattaga ttcaatgtga acgattagtt tgctatatct taattttgcc ttttgcaaag
    49561 gccatctctc gtttatttac ttattttagt aaatgatggt gcttgtatat atacatggcg
    49621 aattaatgga tattgcagat gtaatattca cagggatcac tgtaattaaa ataaatgaag
    49681 gattatgtaa tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag
    49741 ccagtaaaac gtactactcg cgcttatcgt gaagaagcaa ttattaaatc cggtatgaac
    49801 ccgttcctgc tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg
    49861 gcggtaaccc aaagtatgca ggcagcgatg atgcgcggcg acgaagccta cagcggcagc
    49921 cgcagctact atgcgttagc cgagtcagtg aaaaatatct ttggttatca atacaccatt
    49981 ccgactcacc agggccgtgg cgcagagcaa atttatattc cggtactgat taaaaaacgc
    50041 gagcaggaaa aaggcctgga tcgcagcaaa atggtggcat tctctaacta tttctttgat
    50101 accacgcagg gccatagcca gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa
    50161 gccttcgata ctggcgtgcg ttacgacttt aaaggtaact ttgaccttga gggattagaa
    50221 cgcggtattg aagaagttgg cccgaataac gtgccgtata tcgttgcaac catcaccagt
    50281 aactctgcag gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg
    50341 aagaaatacg atattccggt ggtaatggac tccgcacgct ttgctgaaaa cgcctatttc
    50401 atcaagcagc gtgaagcaga atacaaagac tggaccatcg agcagatcac ccgcgaaacc
    50461 tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt accgatgggc
    50521 ggcttgctgt gcatgaaaga cgacagcttc tttgatgtgt acaccgagtg cagaaccctt
    50581 tgcgtggtgc aggaagggtt cccgacatat ggcggcctgg aaggcggcgc gatggagcgt
    50641 ctggcggtag gtctgtatga cggcatgaat ctcgactggc tggcttatcg tatcgcgcag
    50701 gtacagtatc tggtcgatgg tctggaagag attggcgttg tctgccagca ggcgggcggt
    50761 cacgcggcat tcgttgatgc cggtaaactg ctgccgcata tcccggcaga ccagttcccg
    50821 gcacaggcgc tggcgtgcga gctgtataaa gtcgccggta tccgtgcggt agaaattggc
    50881 tctttcctgt taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg
    50941 ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa
    51001 gcctttaaac atgtgaaaga gaacgcggcg aatattaaag gattaacctt tacctacgaa
    51061 ccaaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat actacagagt
    51121 ggctataagg atgttagcca ctctcttacc ctacatcctc aataacaaaa atagccttcc
    51181 tctaaaggtg gcatcatgac tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt
    51241 atggttatag caggtacagt aattggtgga ggtatgtttg ctttacctgt tgatcttgcc
    51301 ggtgcctggt ttttctgggg tgcctttatc cttatcattg cctggttttc aatgcttcat
    51361 tccgggttat tgttattaga agcaaattta aattatcctg tcggctccag ttttaacacc
    51421 atcaccaaag atttaatcgg taacacctgg aacattatca gcggtattac cgttgccttc
    51481 gttctctata tcctcactta tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg
    51541 atatcaatga atttgggcta tcacgctaat ccacgtattg tcgggatctg cacagccatt
    51601 ttcgttgcca gcgtattgtg gataagttcg ttagccgcca gtcgcattac ctcattgttc
    51661 ctcgggctga agattatctc ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat
    51721 tactccatcc tgcgcgatgc caccagcacc actgcgggaa cgtcttactt cccgtatatc
    51781 tttatggctt tgccggtgtg tctggcgtca tttggtttcc acggcaatat tcccagcctg
    51841 attatttgct atggaaaacg caaagataag ttaatcaaaa gcgtggtatt tggttcgctg
    51901 ctggcgctgg tgatttatct cttctggctc tattgcacga tggggaatat tccgcgcgaa
    51961 agctttaagg cgataatctc ctcaggcggc aacgttgatt cgctggtgaa atcgttcctc
    52021 ggcaccaaac agcacggcat tatcgagttt tgcctgctgg tgttctctaa cttagctgtt
    52081 gccagttcgt tctttggtgt cacgctgggg ttgttcgatt atctggcgga cctgtttaag
    52141 attgataact cccacggcgg gcgtttcaaa accgtgctgt taaccttcct gccacctgcg
    52201 ttgttgtatc tgatcttccc gaacggcttt atttacggga tcggcggtgc cgggctgtgc
    52261 gccaccatct gggcggtcat tattcccgca gtgcttgcaa tcaaagctcg caagaagttt
    52321 cccaatcaga tgttcacggt ctggggcggc aatcttattc cggcgattgt cattctcttt
    52381 ggtataaccg tgattttgtg ctggttcggc aacgtcttta acgtgttacc taaatttggc
    52441 taaatccttc aagaagccag ccattcgctg gcttcttgcc tctcaggaaa tcacttatgt
    52501 ccaaatggca actcgcctga ttctccttca ccacgtatgc tttgcgtcac cttactatta
    52561 ggacgcttta gcccatgtcc cgctttttaa tttgtagttt tgccctggtt ttactttatc
    52621 ccgccgggat tgatatgtac ctcgttggtt taccgcgcat cgccgacgat ctcaatgcca
    52681 gcgaagcgca gttgcatatt gcgttctccg tttatctggc gggcatggca gctgcgatgt
    52741 tatttgccgg taaagtggcc gatcgttcag ggagaaagcc tgtcgccatc cccggcgcgg
    52801 cgctatttat tatcgcttcg gtgttctgtt cactggctga aaccagcacg ttatttcttg
    52861 caggccgatt tctacagggg ttgggcgcag gctgttgtta cgtagtggcg ttcgccattt
    52921 tgcgcgacac gctggatgat cgacgtcggg ctaaagtgct gtcattactc aacggtatta
    52981 cctgcatcat tccggtgtta gcgccagtgc tcggacatct gattatgctt aaattcccgt
    53041 ggcagagtct gttctgggcg atggcaatga tgggcatcgc ggtactgatg ttgtctttgt
    53101 ttattttaaa agaaacgcgc ccagcgtccc ccgctgcttc ggacaaacca cgagaaaata
    53161 gcgagtcgct gcttaatcgg tttttcctca gccgtgttgt tatcaccacc ctcagcgttt
    53221 cggtgatcct cactttcgtc aatacatcgc cggtattgct gatggaaatc atgggttttg
    53281 agcgcggaga atacgccacc attatggcgt tgactgctgg cgtcagcatg accgtttcat
    53341 tctccacgcc atttgcgctg ggaattttta agccacgtac gttgatgatc acctcgcagg
    53401 tgttattcct tgcagcgggg atcacccttg ccgtttcacc ttcccatgcg gtttctctgt
    53461 ttggtatcac gctgatttgc gccggtttct cggtaggttt tggcgtagcg atgagtcagg
    53521 cgttaggacc attttcatta cgcgcgggcg tagccagctc gaccttaggt attgcgcagg
    53581 tttgcggttc gtcactgtgg atttggctgg cagcggtggt tggtatcggc gcatggaata
    53641 tgctgatcgg gattctgatt gcctgtagca tagtgagcct gttgctgatt atgttcgtcg
    53701 cgcctggacg ccccgttgcc gctcatgaag aaatccatca ccacgcttga tctcaatctg
    53761 ctgctctgtc tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc gaagcggatg
    53821 aacgtgaccc cttcagcggt gagtaagtcg ctggcaaagt taagagcgtg gtttgacgac
    53881 ccgctctttg tgaactcacc gctgggcctg tcgcccacac cgctaatggt cagcatggag
    53941 caaaatctgg cggaatggat gcaaatgagc aatctgctgc tggataaacc gcaccaccag
    54001 acaccgcgcg gcctgaagtt tgagttggcg gcggaatcgc cgctgatgat gatcatgctt
    54061 aatgcgctgt cgaaacggat ctaccaacgt tacccgcagg cgaccatcaa actacgtaac
    54121 tgggattacg attccttaga tgccattact cgtggtgaag tggatattgg tttttccggt
    54181 cgcgaaagcc atcctcgctc gcgggagctg ttaagctcgc taccgttagc cattgattat
    54241 gaagtgctgt ttagtgatgt accctgcgtc tggttacgca aagatcatcc ggcactgcat
    54301 gaaacgtgga atctggacac ctttttacgt tatccgcata tcagcatttg ctgggaacag
    54361 agcgacacct gggcgctgga caatgtgtta caggagctgg ggcgcgaacg cacgattgct
    54421 atgagcctgc cggaatttga gcagtcactg tttatggcag cgcaacccga caatctgcta
    54481 ctggcgaccg cgccgcgcta ctgtcagtac tacaatcaac tccatcaact gccgttggtt
    54541 gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagttcc ttttaccctg
    54601 ctgtggcata aacggaacag ccataatccg aagatcgtct ggttacggga aaccattaaa
    54661 aacctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgacccaat ttcacaataa
    54721 aatgtaaaaa agttgtaata agcttgtctg aatcgaactt ttagccgctt tagtctgtcc
    54781 atcattccag taaatgatta ctcttgtatt cataatggac cattaagcat ggagcgaaaa
    54841 atggcgactc actttgcccg agggatttta acggaaggac atctgatttc tgttcgtctc
    54901 ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag ccgttttctg
    54961 gcgtctagag gtttactcgc agaactgatg ttcatgctgt atggcattgg cgaattgccg
    55021 gaaatcgtca ccctgccgaa aggtaaaccg gttttcagtg ataaaaattt gccttcgttt
    55081 tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg tgaatgtggt
    55141 ctcgatatgg aactacagcg tgcgacgcgc gggtttcaca gcccacacgc gcccgataac
    55201 cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc taacgaagcg
    55261 cgggcgcagc tcatcacgct gcgccgaagc gtgctaaaac taaccggtga tgttttgaat
    55321 gacgatccgc gcgatctgca gctgctgccc attgccggac gcctgaaatg tgctcatgta
    55381 aatcatgtag aagcgttatg cgacgcggag gacgtgctgg tgtggtccgt ggcggtcacg
    55441 cccacgattg aaaagctcag tgtctgggag ttcgatggca aacacggctg gaaaagcctg
    55501 ccggatattc acagccgcgc caacaatcct accagccgga tgatgcgttt tgcccagctc
    55561 tctaccgtga aggctttttc gccaaattga tagacaacca caggagtcat catgtctgaa
    55621 aaattgcagg tggttacgtt actggggagc ctgcgcaaag gctcatttaa tggcatggtt
    55681 gcacgtaccc tgccgaaaat tgctccggcg agcatggaag tcaatgcgtt accttccatt
    55741 gccgacattc ccttgtatga cgctgacgta cagcaggaag aaggttttcc agcaacggtt
    55801 gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac gccggaatat
    55861 aactactcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg cctgccggat
    55921 caaccgctgg ccggtaaacc ggtgttgatt cagaccagct caatgggcgt gattggcggc
    55981 gcgcgctgtc agtatcacct gcgccagatt ctggttttcc tcgatgcaat ggtgatgaac
    56041 aagccggaat ttatgggcgg cgtgattcag aacaaagttg atccgcaaac cggagaagtg
    56101 attgatcagg gtacgctgga ccacctgacc gggcaattga ccgcatttgg tgagtttatt
    56161 cagcgagtta agatctaaat aaaaaacccg ccagcaatca tgcatggcgg gtttttaacg
    56221 cgctatcgat tttagtgagc gtcgataaag acaatcttca ggataaacag cagcgcaacg
    56281 atgattacgc acgggcttag atcacgcaga cgtccggtac cgattttcat cacgcagtag
    56341 gagataaagc ccagcgcgat accttcggta atcgagaagc tgaacggcat catcacggcg
    56401 gtaataaacg ccggaacaga ttcagtaaga tcctgccagt tcacgcgtgc cagactggaa
    56461 gtcatcagca cgccaacgta aatcagcgca ccagctgcag cgtagcctgg caccatcccc
    56521 gccagcggcg acagaaagat aaccagcagg aacagcagac caacaaccac tgccgtcaga
    56581 ccggtacgac cgccaaccga tacgccggaa gaggactcaa tataagccgt aacggaagaa
    56641 gtaccgataa acgaaccggt cacggaagag atactgtcga catacagcgc ctgcttcatg
    56701 cgcgggaatt tacccttctc atccgccagg cctgctttat cggtcacgcc aatcagcgta
    56761 ccggaggagt caaacaggtt gaccagcatg aaagagaaaa tcacccctgc cagcccgagg
    56821 ttaaacgacc cggctaaatc tacatgaccc acaactgtca ttacgctcgg cggcgcagaa
    56881 acgatgccat tgtagtgcac atcacccagc atccagccca gcagcgtcgt caccacgata
    56941 gaaaccagca ccgctgcgtg aatgttgcgc gaggccagaa tagcaatgat gaagaagccg
    57001 aggatcccca gcagcacgct gtgagaagtc aggttaccga tgctgaccag cgtttccggg
    57061 ttagcgacaa tcacacctgc attcttcagt cccatcatac caatgaacag accgataccg
    57121 ctggtgatgc ccacacgcag acttaccgga atgttggcaa tcatccagta gcgaacgcgg
    57181 aaaatcgtca gtaacagcag accaatcgca ccccagaaga ttgcgcccat cccgacttgc
    57241 cacggcaagc ccatcgcctg tacaacgaca aaagcgaaga acgcattcag gcccatagcg
    57301 ggtgccagtg caactggcag gttagcaaac agtcccatca taatactgcc gaatgcagcg
    57361 atcagacagg tagtgacgaa gacggcgctg gtatccatgc cagcaacgcc aagaatttgc
    57421 gggttaacaa aaacgatgta aaccatcgtc aggaaggtgg taaaaccggc gatcacttcg
    57481 gtccgtgccg tcgtgccatg ttcgcgcagt ttaaacacgc gttccagcat cccctggcca
    57541 gaagcctggg ttgtgtgttg ttgactcatt atctatttcc gaacaaggag ggaaaatccg
    57601 tcgctatcgt ataccaaaat gagacaatat ggacggctga agataacttt ttttcattgt
    57661 atttgcttat acggcaacga ttgcgtcatg cgaattggca tgacgaaaac gttaaactaa
    57721 cgataaagga aagacatgtc ccagatagaa gcggtatttt tcgactgcga cggtacgctg
    57781 gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt
    57841 acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc
    57901 gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga acacgtttac
    57961 cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctggagcg
    58021 ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg
    58081 cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc
    58141 tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg
    58201 aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt
    58261 atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt
    58321 cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt
    58381 ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg
    58441 ctcaaaggta agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga
    58501 ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc
    58561 ttcgcaaaag aaccgctggt tcttttgcga agaatacagg tacttccatt gtttctctta
    58621 ttgtcgatta ctacgggagt tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg
    58681 ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc
    58741 gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag
    58801 ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg
    58861 gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct
    58921 gaaacagact ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag
    58981 gatgttacta tgactcaaaa tatcaggccg ttaccccaat tcaaatatca tcccaagcca
    59041 ctggaaacag gcgcatttga acaggataaa accgtagagt gcgattgctg tgaacaacag
    59101 acgtcagttt attactcggg tcccttttat tgcgttgatg aagttgaaca tctctgtccg
    59161 tggtgtattg cggacggttc tgctgctgaa aagtttgcag gtagttttca ggatgatgcc
    59221 agcatagaag gtgttgaatt tgagtatgat gaagaggacg aatttgccgg tattaagaac
    59281 acatatcctg atgaaatgct gaaagagttg gttgaacgca cgccaggtta tcatggatgg
    59341 caacaggaat tctggcttgc gcattgtggc gatttctgtg cttttatcgg ctatgtgggc
    59401 tggaatgata taaaagatcg cctcgatgaa tttgccaacc ttgaagaaga ttgtgagaat
    59461 ttcggtatta gaaattctga tctagctaaa tgcctgcaaa agggtggtga ttgtcagggt
    59521 tatctcttcc gctgtctcca ctgcggcaag ctgagactgt ggggtgattt ttcgtagtta
    59581 tttaaataat gagaacaggc cggagcgtaa ttcatacatc cggccttatt tcttcagctt
    59641 aaccgagcgc taattctgcg gcagcggctt tatcagcgat taccatcagc gaaggatgca
    59701 gctgcaacac ggaagctgga acatcttcgg taaccggacc ttgcagaaca tttttcagtg
    59761 cctgcgcttt acctgcgcca ctgacaatta tcagcaagtt tttcgccgcc atgatgcttt
    59821 tcggccccat cgtcacgtag ctgtcaggca ctaacgaaaa gtcgccgccc aactcaccat
    59881 gcgccacaat atcaaccatc tctccctgga tcgggaactc cacggtttgc tcatggaaat
    59941 gggtcgtatt cggcaaattg ccacaaaaat gaccatctgc acctaatccc agcaccacca
    60001 aatccaggcc gccttcacgc gccagtttct gatcatgctc gcggtagtta tcaatagtga
    60061 gcttctggat attctcttct ttgatccccg caggggtgaa aaacagatta cgcagattgg
    60121 taatcgttac accttctccc tctttgccgc gaaagggaat ttcatcgaaa ttatagaaat
    60181 agcagttatc gtaccagggc ttacctttaa ccagggtggt gaggtattca tacatgcctt
    60241 tgggcgtgct accggcggta attgccaggt taacacgacg cgtcttcgac atataaccaa
    60301 gcagatggtg tgccgcgaca cggctcattt cctggtaatc ttcggtaatg attaatttca
    60361 tcttagtgcc ttattttatt taaataattt ctgtacgaag gctgcatagg caggccgcca
    60421 gacatccatt tcatggttca ggccgggata ttcctggtaa tcaaacttaa tttttttctg
    60481 ctcaagctca gttttcagcc cggcgatatc cttgccggtt acgacatctt tatcaccaac
    60541 gaccacagta aaattacgta gttgctggtt aatagctgcc ggatcgttca gccgggccgc
    60601 gacaccttca tccggtacgg ttgtcgtggt aacaccactg aatgtggcca gccagccaaa
    60661 gctttccaga tgattcattc ccgataccag cgcctgatac ccaccttgtg aaagccctgc
    60721 cagtgcgcga ccatcggcat ctttactgac gttaaaacgc ttgctaatca gcgggataat
    60781 atcgttcatc agttcgcgat ctgccgcttt agcattcagc ggataaaaga ctttacgtct
    60841 ttcctgaggc acgaaatctt cgggaataat gcccttcgca tcggtttcgg tatccgggat
    60901 caccaccagc atcggtttaa ttttcccttc agccagcagg ttatccatga tttgcgggat
    60961 acgcccctga tcgatagcgg aacgtccggt atcaccaaaa ccgtgataga aatagagcac
    61021 tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc cagacataca tctgacgttc
    61081 agattgcaat gcgttggagt ggtaagttat ggcgatcaaa tcaccatgcg caacagaacg
    61141 cgtatccaga taactgcctg gcaccagaat catactggag ttaacctggc gctgaggctt
    61201 agtcattgcg gtgcctgtat caatgctgcg tacaccatca acattgaaaa aatactcgta
    61261 caggttgcct ttcaggatag gtgtgcgcca cgaccagact cctgcttcgt ctttggtcat
    61321 cgggtgaata ttgtccagaa ccggaacacc caccactaca gagacatttt ttgccccagg
    61381 ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc tgagtcacat actgtttcac
    61441 cgggatagtg ggtgccgggc tggctggcat atccgctgct atggcccact gagcagaaat
    61501 accgctggca atagccagcg ttaaagcagc aatttttata ttcatcgtaa tattcctttc
    61561 attaagttcc attaccacca gatttcagcc tgggcaccga cagcaaactg gtcgtcttta
    61621 ttgtcttcga aggtgaagcc atccagttcg ttttccaggg ctttgatata cgtggcgtag
    61681 aaacgaattt ccggacgcga ggtcaacata ctggtattga ctttaaaggt atggaagagc
    61741 gtggttttat aaccagactc attaaattta ttactattcg catctttgtt ttgctgggtg
    61801 aaatagccca gttcaacacc tgtctggtta tattggtccc aaatataggc cgggcgaaca
    61861 accgcacgaa tagattcgaa atcagagtgt gcgcctgttt cgtagctata aatatcgtta
    61921 ccgaaggagt aaacaatcgc gttagccaca atgaaatgat cgccgatata ggcttcgccc
    61981 tgtgaagtca gacgaactgc tgttccgccg gtgtgatcac cataataacg accattaaag
    62041 gtggtaaatg gacttgcgcc agcataacgg ccaaagttac tggcgataga gttattcgca
    62101 accaggaagg agaattcgtt gaagccacct ttatcaaatt tctgcgttaa agatgtgcca
    62161 aacatccagg tatctttcca gtcataatag ccgttattat cctgattatc tttttccgat
    62221 gcgctttcgt ttgccgtgac ataacgacca ctcaccatta aggtcgcttt atcccataac
    62281 gggatatctt tatagcgtaa atcaatagca ttggtattaa tctgctgttt gttttgcagg
    62341 ctgcgatcgt aatcatcgac atcttcgcga accagcgcga tatcaatttt acccggaccg
    62401 actttccagt tttccagacc tacacccgct gcggcatcag tacgctgcgt tttccagtca
    62461 agcatctgga tttcaatttt cggcgcaccg tgtttaccca cccagaaatc agcctctggc
    62521 gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg agaactgcat aaagttctcg
    62581 ccaccggcgt tatcgccaaa ccagccggta gagtactgct gaccaacgtt accatccatc
    62641 atcacaacgg catcaacccg tttgccgttt tcgttgtaga cacgttgttt taactgcaaa
    62701 tcaaaccagc cggagtattc gttaccaaag cggcccagag aaccaatcgc ccatgattta
    62761 ggtgaaccat gagaggcggt cccccaacca gaacggtaat agccgttgta actaaagcca
    62821 atttcatctt tcacaaactt actgaaatct ttcagagtca tggaagaata aatcgagcct
    62881 gtctcactgg catttttctc ttcatttttg accagtacag catcaggttt tgcggcacta
    62941 ctggtcggga acggattggc ggcataccct tgatcattcg tacttacgct acgattcacc
    63001 gtcgctggcg tatatttttt cttctcttca tctttatact ttttgagttc gctttgcgtt
    63061 tctcttaacg ctttttctaa taactcaagg cgatgttcca ccgttaatga ttcagccaat
    63121 gattgtgcag aaaaggctaa cggtgccatt aataagatgg cagaggtaat aagatttcgt
    63181 ctaaacataa tatcccttta tggtgcaaag aaagaattaa cgtatcgcat ccagactgtt
    63241 ctgaatgcga cgataattaa ggtgctttat tggttatttt tttaatgaca gcccccgcgt
    63301 tttgatcact tctgcatacc atccgaagct tttcttacgc gttcttgtga ggcttccttc
    63361 gccattatca tcacgatcca cataaataaa gccgtagcgc ttagacattt gtgaatgaga
    63421 tgcactgact aaatcaattg gcccccaact ggtgtacccc ataatatcca caccatcggc
    63481 aatcgcttca tttacctgta ccaggtgatc gtttaaatag gcaattcgat aatcgtcctg
    63541 tatcgaacca tccgcttcaa cgctgtcttt tgcgcctaat ccgttctcga caataaataa
    63601 cggtttttga taacgatccc aaagcgtatt taacagaacc cgtaatccaa ccggatcaat
    63661 ttgccacccc cactctgaac ttttcagatg cggattgggg atcatattca gtatgttgcc
    63721 ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccagtcatgt aataactaaa
    63781 agagatgaaa tctacggtat gttttaaatc ttctgcgtca ctttcagtca tctcaatggt
    63841 gatattgtgg tcgcggaaga aacgctgcat atagccggga tactgaccac gcgcctgaac
    63901 atcaccaaag aacatccagc gccggttctc ttccatggcc tccaacatat cctgtggctg
    63961 gcaggtgagg gggtaaacca gcccgccgag aagcatattg ccgattttcg cttcggggat
    64021 caggctatga caggctttaa ctgcccgcgc actggcaacc agttgatgat ggatcgcctg
    64081 ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg gcgcgtgtaa
    64141 cgacatattg atttcattaa acgtcagcca taacgctact ttatgttggt agcgggtaaa
    64201 gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc cgattagccc aaccgccgta
    64261 gtttttcacc agcccatatg gcatttcgta atgggataac gttaccagcg gcttgatccc
    64321 cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct aaccccgctt cattcggttc
    64381 ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg gaaatacgca gacaggtgaa
    64441 gcccatctcg gcaaataacg cgatatcttc cgggtaacgg tgataaaaat cgatggcgac
    64501 atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccatta cgccatgagg
    64561 ctgtaaatct gaggtcgtga tccctttgcc atcttccagc caggcacctt ccacctgatt
    64621 ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat gctttcataa ttaactcctt
    64681 ttatcgttag cgaatgatgg ataacagcgg ctcaccagcg cttatctgcg ccgtgccgtg
    64741 gggtaatacg tctgtaaaat catcgctatt actgattaat accggcgtcg tcagatcaaa
    64801 tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg tattgacctt
    64861 gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg tgtcgatacc
    64921 gacatgaatc aggatctcca caccatcatc tgactcaatg ccaatagcgt gtaatgtggc
    64981 gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact tcaccaaccg agggcagaat
    65041 ggcgatacct ttacccaaca ggccactggc aaacctggta tcagcgacgt gaatgagcgg
    65101 cacaatctct cccgtcatcg gtgaacagat accgccctgc tcaggtggtg taataacctc
    65161 tggtgttttc tcttgcgggg caccctgcgc tggctgacgt ttagcggtga tgaaatgaag
    65221 catcaccgta ccgacaaatg cgcaaccgat ggcaatgaca ccgccaataa cgctggccca
    65281 gacggtgaaa tcaatccccg ttgacgggat ggtttgcatg aaggtgaaaa tacttggcaa
    65341 accaaaggag taaactttcg tttgcgcgta gccaatgatg gtggccccca atgctccact
    65401 gatacaggcg ataacgaagg gatacttacg cggcaggttg acgccatata ccgctggttc
    65461 ggtgatacca aacaggcccg tcaacgccgc tgatcccgcc accacttttt tctgcgcatc
    65521 gcgttcgcag aggaagacgc cgagcgccgc cccgacttgc gccataatgg cgggcattaa
    65581 cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacaca gcggcaccag
    65641 gccccagtgc agtccgaaca tgacgaagat ttgccagaaa ccgcccatta ccgcgcccgc
    65701 aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcattt cgcttatcca
    65761 ggttgatagc ggccccacca gcaaaaaggt gacgggtgtg ataaccatca gacatagcaa
    65821 tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc gttccagaat
    65881 gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat tcaacaatgt
    65941 gaccggaata cccaggaaat ccagccccag ctcatccgct ttttgcccgt tctcgaaagc
    66001 agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa atggattacc
    66061 gccgaagcgt ttccccgcgg tgtatcccag gattatcggg aagaaccaaa acaaggcatc
    66121 actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact gaaaagtgag
    66181 cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcaaaccga tcagaggcgt
    66241 aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttat catcattttc
    66301 cggtgcctgt tgcgcttttt cgtcaaggcc tgccacactg ttaaccgcca ggaagacatc
    66361 ggccacatgg ttacctatga ccacctgaaa ctggccaccg ctttccacca ccataataat
    66421 accgggggtc tttttcagta cctctgcttg cgctttgctt tcatccttta acttaaaacg
    66481 taatcgcgtt gcgcaatgca tcagactaac aatgttatct gcgcccccga ctcctgcgac
    66541 tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca aaactcaaaa
    66601 aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt ttttcaggtt ttgcccgctt
    66661 agtgcggtaa caatccttta ctcagtaata atatttcagt gttctttgcg cacgcgctct
    66721 atatttatgg ctaaaaacat aatctctgcg ggtgagattt tacgttgata ctgcaaacca
    66781 ataaaaatgg cgatccgctc cgcacattgc catgcttgcg ggtaattttg ttttactgct
    66841 tgttgtaatg actcatcact atcgttaatt gaagcatgtc caagaatacg ccaggataaa
    66901 aacttcagat gcgtaaccag tcgctgataa ctcaagcttt cttcctggta attaaggctg
    66961 aactgaaatt ttattaattg cagcatttcg cgcattaatt gcgtgacacc tgcaacatcc
    67021 tccatatttc cgctcatttg ggcactgacc agatgcatgg caataaagcc cacttcatct
    67081 ttcggtaact gcacgcccaa ccgtttatca ataatggtta acgcttcttc ccctagctgg
    67141 aactctttcg ggtaaagccg ctggatatcc cacagcagcg ggttgggcag taacacgttt
    67201 tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta gcgagatata aatactgtcc
    67261 tgtaattttc ccaagcgctc ctgcgctaaa gagataatac gatcacaggt tgccatcacc
    67321 tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt tcagttcatg actgctcaag
    67381 gcatactctt tttctattcc acttgagtta attctttcgc cagcgcgttt ttgaaagcca
    67441 attccgcgcc ccatgacgac tttttcccgc tgttgatcat caataaccac cacaacatta
    67501 ttgttgagaa ttttggtgat ttgcatgttc atagcaagga ccttttttat aaacaaaaaa
    67561 acccgacttc accagtattc tctggttatg tcaggttttg cctgcgaatg cagtaacaat
    67621 ccagtcattt attaatggtt tttataacga acatccaggt tcggaaatta atttaattgc
    67681 gtgcagagaa aatagcaatg cgctattgat aaaaatatga ccatgctcgc agttattaac
    67741 tttgtgtaat tttaggaatt tataaagtta tatataacaa atcccaataa ttaagttatt
    67801 gggatttgtc tggtgaatta tttgtcgcta tctttccccg ccagcagttt atccagctca
    67861 tcgccaccga cgtgacggaa atcctgcccc ttcacgtagt agaagataaa ctcacaaata
    67921 ttctggcagc ggtcgccaat acgttcgata gaacgcgcgc agaacagcgc agtaagtacg
    67981 ctcggaatgg tacgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg
    68041 tattcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc
    68101 cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc
    68161 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
    68221 ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg ttttactgat caccataacc
    68281 agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg
    68341 atcgccactt ccatcatgtt gacgttcttg tcgccttcga tcacgcgctt cgccagatcg
    68401 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc
    68461 atggtcatca cctgcgtgcg gatactttcc agttcggcgt tgaactggcc ggaaatatgt
    68521 ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt
    68581 agtcttctgt ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
    68641 tcaattcgcc caggtacata aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
    68701 tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
    68761 gcccggtaga gatagggtcg agcgccgaac acggttcgtc gagcagcagc acttccgggc
    68821 gaatggcgat accacgcgca atacacagac gctgttgctg accaccagag agagagtaac
    68881 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
    68941 gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa cagacgaacg ccaaaagcga
    69001 tgttgtcgta gatggacatc ggaaacggcg tcggtttctg gaacaccatg cccactttcg
    69061 cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt gtcgccatca agcagaattt
    69121 caccttctgc gcgctgctcc ggatacagtt caaacatttt gttgaaggta cgcagcagcg
    69181 tcgatttacc gcagccggac ggcccgataa acgccgttac ctggttttta gcgatatcca
    69241 ggttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
    69301 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tacctgatgc
    69361 ttttcgctaa tcaggcttac cgtaggccgg gtaaggcgtt cacgccgcat ccggcaaagt
    69421 gttaaccgtg tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcaggacgc
    69481 acagggtaat gatcaatacc ccggcccagg ccagttgttg ccattccgca aacgggctca
    69541 tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc
    69601 agaactggtt ggagagcgcg gtaaacagca gcggcgcggt ttcaccggca atacgggcaa
    69661 tcgccagcag gataccggtc ataatcccgg acaccgacgc tttcagcgta atcgcggaga
    69721 tcatcttcca cttcggtgta cccagcgcat aagccgcttc acgcaggctg tccggcacca
    69781 gtttcagcat gttctcggtg gtgcggataa cgatcggcac ctgcaacagc gccagcgcaa
    69841 ttacgcccgc ccagccggag aagtgctcca tctgtgccac cacgatggtg tagacaaaca
    69901 gaccaaccac aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg
    69961 ccagccagga tttacgacca tattccgcca gataaatccc cgccataatg cccagcggcg
    70021 taccgaatac cgtggcccac aaaattaaca gcccgctacc cgccagggcg ttcgccagac
    70081 caccaccttc cgtattgggc ggcggcgtca tttcagtgaa cagcgccagc gacataccgt
    70141 cgataccgcg agtgatggtg gacattaaaa tccagatcag ccagaacagg ccgaaggcca
    70201 tcgtcgccat cgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt
    70261 tgcggcgaga ttcagccagc gccgcagtgg tttgcatttc aaccatagcc attagcgtgc
    70321 cccctcattc ttagccaggc gcataatcat aaacttcgat gcggcgagga cgatgaaggt
    70381 aatcacaaac aggatcaggc ccagttccat cagtgcggca acgtgcagac cggattccgc
    70441 ttccgcaaat tcgttcgcca gcgcagaggt gatactgttg cccggcatat acagcgaggc
    70501 gctgtcgagc tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc
    70561 gcggcccagc cccagcatga tgccgccgat aacaccattt ttggtgaacg gaagaacgat
    70621 acgccagata acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac
    70681 cggggtttgt tcgaacacat cacgcattac cgccgcaatg tacggaataa tcatgatggc
    70741 gaggatcacg cctgccgcga ggataccgat accaaatgcg gggccagaga acagcgcgcc
    70801 aacaatcggg atattcgaca tgatattgcc gaccggctcc tggaagtaaa cggcgaatag
    70861 cggcgcaaag ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag
    70921 ctcaatggcg atacccagcg ggcgtttcag ccagccaggc gcaagctcag tcaggaacag
    70981 ggcgataccg aaactcaccg ggacggcgat cagcagcgcg ataaacgaag tcaccagcgt
    71041 accgtagatc ggcactagcg ccccgtagat atcgttcggt gcatcccact ctttggtcca
    71101 gaggaaagcc agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat
    71161 aatgccaccc aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat
    71221 tatgtcgccc tttttacccg gtgggttaaa agcaggcttg gttgcagcca taagttactc
    71281 ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg ataagccgtt aatgccttat
    71341 ccggcctgga gttttattag tacagcggct taccgctact gtctttaata ttggtcttcc
    71401 acgcagcgcg aacctgttca actacactat ccggcaggct ggcgtaatcc aggtcgttcg
    71461 cctgtttagc accggttttg tacgcccagt cgaagaattt cagcacttct gtgccttgtt
    71521 ctggtttctt ctgatctttg tggatcagaa tgaacgtggt agaggtaata ggccacgcat
    71581 cttcgccttt ctggttggtc agatcctgag cgaaggtttt gctccagtct gcgccttttg
    71641 ctgcattagc gaagttttct tcggtcggac taaccggttt accatcagcg gagatcagtt
    71701 tggtatacgc caggttgttc tgcttcgcgt aagcatattc aacgtaacca attgcacccg
    71761 gcagacgctg aacgaacgcg gcgataccgt cattaccttt accgcccaga ccgatcggcc
    71821 attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact ttcgccagat
    71881 agctggtgaa gacgaaggag gtaccggagc catctgcgcg gcgtaccacg gcgatgttct
    71941 gtgacggcag tttcagaccc ggattcagtt tggcgatggc ttcatcatcc cacttcttga
    72001 ttttgccgag gtagatgtcg ccgagggttt taccatccag caccagttcg ccagacttca
    72061 gccctggaat gttaacagcc agcaccacgc cgccaatcac ggtcgggaac tggaacagac
    72121 cttcctgagc cagtttttcg tcagacagcg gcgcgtcaga ggcaccaaaa tcaacggtat
    72181 tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta actttattac
    72241 cggtttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg aaggttgcac
    72301 ctgcacctgt caggcttgct tctgcaaaca cagagaaagc gctcatcgat aaggtcgcgg
    72361 cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg aggattcata
    72421 aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag ttatatgtaa
    72481 ggaatatgac agttttatga cagagagata aagtcttcag tctgatttaa ataagcgttg
    72541 atattcagtc aattacaaac attaataacg aagagatgac agaaaaattt tcattctgtg
    72601 acagagaaaa agtagccgaa gatgacggtt tgtcacatgg agttggcagg atgtttgatt
    72661 aaaaacataa caggaagaaa aatgccccgc ttacgcaggg catccattta ttactcaacc
    72721 gtaaccgatt ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca
    72781 tggtaagcca gcagctgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
    72841 tgcggcatct cgatgatgtg catgttatcg ctacttacaa aacccgcatc ctgatcggcg
    72901 aagacataca actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
    72961 aacagttcgt tgttcggtgc aacgacgata accggcatat ccgcgtcaat cagcgccagc
    73021 gggccgtgtt tcagctcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc
    73081 agtttcaatg cgccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg
    73141 tgatgtttgt cagagaaatc ttctgccaga gcttcaatgc gtttgtcctg agacagcatc
    73201 tgctcaatac ggctcggcag cgcctgcaga ccatgcacga tgtcatgttc aatggaggca
    73261 tccagacctt tcaggcgaga cagcttcgcc accagcatca acagcacagt taactgagtg
    73321 gtgaatgctt tagtggatgc cacgccgatt tccgtacccg cgttggtcat cagcgccaga
    73381 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
    73441 cccagctctt tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
    73501 aaggtgatca tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg
    73561 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtaacggga aaccataccg
    73621 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttcg
    73681 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
    73741 gtgtttttga tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg acggtaaata
    73801 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
    73861 ccagttttat cgaagatgtt taccgaacgg cgagtaattt ccgcaatatc gccctcttca
    73921 aggaagataa agcgacgggt caccggcaac agcgccagct ggtcagaagc gataaagttt
    73981 tcgcccatac ccaggccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc
    74041 gggtgacggg agtccatgat cactgtaccg tacgcaccac gcagttgcgg gatagcacgc
    74101 agaacagcct cacgcagagt accgccctgt ttcagctccc agttcaccag atgggcaatt
    74161 acttcggtgt ctgtttcaga aacgaaggta tagccacgcg cttttaactc ttcgcgcagc
    74221 ggttcatggt tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga
    74281 tgcgcattca cttctgaagg ttcaccgtgg gtcgcccagc gagtatgagc aatacccgta
    74341 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctggacttt accgaggcga
    74401 cgcaggcggg tcatatgacc ttccgcatca acaacggcca gaccggcaga gtcatatccg
    74461 cggtattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc
    74521 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
    74581 atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact
    74641 gcttgtggga gggagattat gttatctcct catcccatgt gaccggatta cccggccaga
    74701 atcacttttt ctttaccgga cgacgccagc cttctttctg agtctgcggc acacggctga
    74761 tagctaatgc attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc
    74821 ctttgcctac tgttaccggg gccaccagct gagtgtcgga accgacaaac acatcgtcgc
    74881 cgataatggt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga
    74941 tgttaacgtt atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc
    75001 ctttacccag acgcgctttt ttcatctcaa caaagttacc gacgtgtgca ccttccagca
    75061 actcagcacc aggacgcagt cgggcaaacg ggccaatagt acaggccgct gccagattcg
    75121 cgtcttccac aacggtatac ggactgattt cgcaatcatc gccaatcacg ctgtttttaa
    75181 tcacgcaacc ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa
    75241 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaaa tcaaaacgcg
    75301 ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa
    75361 cgcgctccag acgggagagt tgcaggcggt tattcacacc ttctacttcg cttaaacgtt
    75421 gcggatgaac ggcgacgatt tcacgccctt cctgatacgc cagcgcaata atgtcggtga
    75481 tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
    75541 cgccgttagc gatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
    75601 catctttgtg ctcaacaatg ccggtaactt tgccattttc acgggtgata cgtccataac
    75661 cggtcggatc atccagtttc accgtcagca gaccaatgcc accctgcggt ttagcatcac
    75721 gcaggcgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
    75781 cttcatcatc ggcaaagaaa ggtgcggcct gctgcattgc atgacccgta cccagctgct
    75841 ctgcctgaag cacccagtta aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc
    75901 cgtgaccgaa caccaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat
    75961 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
    76021 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
    76081 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
    76141 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagtgc
    76201 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
    76261 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacagggggg
    76321 ttttatatca gcaggatcta tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc
    76381 cagcctgttt ccagactggc ttttgtgctt ttcaagccgg tgttacatcg cttttttggt
    76441 caactcgata acgcgcagct gcgcgatcgc tttggccagt tccgcagacg cctgagcgta
    76501 atctacgtcg ccgtgagagc tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc
    76561 tcgcgcttcg tcgagatcct ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc
    76621 aggctgcact tcaagaatgc cgccagacag atagataaac tcttcgtgac cgtgctgttt
    76681 cacgatgcga atcataccag gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat
    76741 ccccagttca ccttcgctac ccgttacctg gattttctcg accagaccag agaacatttg
    76801 ttgctctgcg ctgacgacgt ccaggtggta agtcattgcc atatcaccct ccgattaagg
    76861 cgttaaagtt ttttggcttt ttccacagct tcttcgatgg aaccgaccat gtagaacgcc
    76921 tgctccggca ggtgatcgta ttcgccttcc atgatgcctt taaagccacg gatggtgtct
    76981 ttcagggaga cgtatttacc cggagaaccg gtgaatactt ctgccacgaa gaacggctgg
    77041 gacaggaagc gctggatctt acgagcacgc gctaccacca gtttgtcttc ttcagacagt
    77101 tcatccatac ccaggatggc gatgatgtct ttcagttcct gataacgttg caggatggac
    77161 tgaacgccac gcgcggtgtc gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg
    77221 ctggtggagt ccagcgggtc aacggccggg tagataccca gagacgcgat ctgacggctc
    77281 agtaccacgg ttgcgtcaag gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca
    77341 tccgcaggta cgtatactgc ctgtacggag gtgatagaac cagttttggt ggaggtgata
    77401 cgttcctgca gaacgcccat ctcttccgcc agggtcggct gataacctac cgctgaaggc
    77461 atacggccca gcagtgcgga tacttccgta cctgccaggg tgtaacgata gatgttgtca
    77521 acgaacagca gaacgtcacg accttcgtca cggaatttct cagccatggt cagaccggtc
    77581 agagcaacgc gcagacggtt tcccggcggc tcgttcatct ggccatacac cagggatact
    77641 ttgtcgataa cgttggagtc ggtcatttcg tggtagaagt cgttaccctc acgagtacgt
    77701 tcacctacgc ccgcaaacac agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc
    77761 tccatcatgt ttacggtttt acctacaccc gcaccaccga acagaccaac tttaccgccc
    77821 ttagcgaacg gacacatcag gtcgataact ttgataccgg tttccagcag ttcctgagag
    77881 tttgacagct cttcgtagga aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg
    77941 atctcgcctt tcatgtcgac cggttcaccc agtacgttca tgatacggcc cagagtcgct
    78001 ttacctaccg ggacttcgat cgggtgttcg aggtctttta catccagacc gcgacgcaga
    78061 ccgtcggagg aacccattgc gatggtacgc acgataccgc cgccgagctg ctgctgaact
    78121 tccagcacca gacgctcatt accattttgc acctcaagag catcgtacac gcgcggtacg
    78181 gcatcctgag ggaattcgac gtcaactacg gcgccgatta cctggacaat ctttccagta
    78241 gccatcttaa atcctctacg aaataacctg tttaaaccgc ggcggccccc gagacgatct
    78301 cggtgagttc ctgagtaatg ctggcctgac gagctttgtt gtataccaac tgcagctctt
    78361 taatcaggct gccgccattg tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct
    78421 cgctggccag gttttcaacc acgccctgat aaacctgaga ttcgacataa cgacgcagca
    78481 gggtatccag caacgccttc ggatcgggtt cgtacaggta atcccaggat ttatgtttca
    78541 gatcatcatc atctgatgcc ggtaacggca gcagctggct gatggtcgga acctgagaca
    78601 tggtgttaat aaatttgttg ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg
    78661 cctgcaacat cacttttacc ggaccgatca gttcggacag ggaagggtta tcccccatgc
    78721 cggtgacctg ggcaacaaca ttgccgccca cggagttgaa gaacgacacg cctttcgagc
    78781 cgatcattgc gaggtcgcat tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca
    78841 gttttttgaa caggttaatg ttcaaaccac cgcacaaacc acggtcggtc gacaccacca
    78901 ggtagcccac gcgtttaacg tcgcggtctt ccaggtaagg gtgcttatat tccagattac
    78961 cgtgtgcaag gtgaccaatc actttgcgca tggtttctgc ataaggacgg ctggccgcca
    79021 tgcgatcctg cgatttacgc attttggaag cggcgaccat ctccatcgct ttagtgatct
    79081 tttgcgtgtt ctggacgctt gcgatcttac tacgtatctc ttttgcgccg gccatgagct
    79141 tctcctcaat gccttgcggc ctgccctaag gcaagccgcc agacgttacc aggattgggt
    79201 tgctttgaag gaatcgagga tgcctttcag cttgccttcg atttcgtcgt tgtagccacc
    79261 ggtctggttg atctcttgca tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc
    79321 ggcttcgaag ctgccaattt tcgacagttc aacatccgcc aggtaaccac gttctgctgc
    79381 gaacagaacc agagactgct gcgcaacgga catcggcgca tactgtttct gtttcagcag
    79441 ttcggtcact ttctgaccgt ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc
    79501 aaactgagag aacgctgcca gttcacgata ctgtgccaga gcggtacgga taccaccgga
    79561 cagttttttc atgatcttgg tctgtgctgc accaccaaca cgggatacgg aaatacccgg
    79621 gttaaccgca ggacgaatac cggcgttgaa caggttggtt tccaggaaga tctgaccatc
    79681 ggtaatggag attacgttgg tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat
    79741 aatcggcagt gcggtcagag aaccggtttt ccctttcact tcacctttgg tgaaggcttc
    79801 aacgtattcg gcgttaacac gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac
    79861 gtcgcccggg aatgcttcac gtcctggcgg acgacggagc agcagggaga tctgacggta
    79921 agcaacagcc tgtttagaca ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg
    79981 gaagtattcg cccattgcgc aaccggcata cggtgccagg tattgcagtg cagcggattc
    80041 agacgcggtt gctaccacaa cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg
    80101 taccacgtta gaaatggtgg acgctttctg gccgatagcg acatagatac atttgatacc
    80161 ggaatcgcgc tggttgatga tggcatcgat agccagtgcg gttttacctg tctgacggtc
    80221 accgatgatc aattcacgct gaccacgacc gattgggatc atggagtcaa cggctttata
    80281 accggtctgt accggctgat ctacggactg acgttcgata acgcccggag cgattgcttc
    80341 tacagcagag aagccgtcgt gatccagcgg acctttaccg tcgattggtg cacccagagt
    80401 gttaaccaca cggcccagca ggccacggcc aaccggaact tccaggatac ggccagtaca
    80461 cttaactttc atgccttcgg caaggtcagc gtacggaccc ataacaaccg cacctacaga
    80521 gtcgcgctcg aggttcagtg cgatagcgta acggttaccc ggcagggaga tcatttcacc
    80581 ctgcatacaa tcggccaggc cgtgaatgcg gataacaccg tcacttacag aaacaatagt
    80641 accttcgttg tgagcttcac tcacaacatt gaactgagca atgcgctgct tgatcagttc
    80701 gctgatttcg gtggaattca gttgcatgct ccagtcccct taagactgca agacgtctgc
    80761 aaggcgctca agacgaccgc gtacgctgcc atcaatgacc atatcacccg ctcggatgat
    80821 aacgcctgcc attacagact tatcgatttt gcaattcagc ttaactttgc gtgacagacg
    80881 tttttccatc gcagcagaaa ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat
    80941 gacgtctacc tcagcggtag cctcactcac ggcacgaagg tgaataaact gctccagaac
    81001 atccgggagc gcgttaagac gaccattttc agccattacc cgaatcaggt tctgaccgtt
    81061 ttcgtccagt tgctcaccac aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag
    81121 cgcgccagag agaagctctg ccatttgttc gtttttggtt acctcggcgg caaacgccag
    81181 catgtcctgc cagcgttcta cactttggtg ttcgacggca aagtcaaaag ctgctttggc
    81241 gtaggggcga gctaccgtaa taaattcaga catcagcccc tccctcctta cagttcagcg
    81301 acaagtttat ccacgatgtc gctgttagca gcttcatcca cggaacgttc gatgatcttc
    81361 tcggcgccag caacagccag gatagcaact tgcttacgca gctcttcacg ggcacgttta
    81421 cgctcggctt caatttctgc ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc
    81481 tcagctttcg cttcgtccag aatctgcgag cggcgtttgt tcgcctgctc gatgattacc
    81541 tgggcttccg ctttcgcttt tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg
    81601 tccttatgtg ctcgttctgc ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg
    81661 atggctgcca ttaatggcgg ccatacgtac ttcatgcaga acagaacgaa caggacaaac
    81721 gcgatggcct ggccgaggat tgttgcgtta agattcacag cacaatgcct ctatttagtt
    81781 aacgttctga tattgctctt taaataaaag caacgcttac tacgcgacag cgaacatcac
    81841 gtacagaccc agacctacag cgatcatcgg gatagcatcc accagaccca taacgataaa
    81901 gaactgagta cgcagcagag gaatcagatc aggttgacgc gctgcgcctt ccaggaattt
    81961 acccccgagg atgccgatac cgatcgcagc accgattgcc gccagaccca tcatcacagc
    82021 ggcagccatg tacagcagat ccatattcag gttttccatg acagtctcca gtttgtttca
    82081 gttaaaacgt agtagtgttg gtaaattaat gttcttcaga cgccatcgac agatagacga
    82141 tcgtcagaac catgaagatg aaggcttgca gcgtaatgat caggatgtgg aaaatggccc
    82201 acggcacatt caggatccac tgtgaccacc acggcaacag accagcaatc agaatgaaaa
    82261 tcagctcacc ggcatacatg ttaccgaaca gtcgcaaacc aagtgaaact ggtttggaca
    82321 gcaggcttac cccttcaagg attaagttga caggaatgaa cgcccagtga ttgaacggct
    82381 gcagcgtcaa ctctttcgtg aagccgccga tgcctttcat tttgatgctg tagaacagaa
    82441 tcaggataaa tacgcccaat gccatagaca gcgttacgtt cacgtccgca gacggaacca
    82501 cacgcagtgc aggcagaccc agtacatgtt cagcaatgta cggcagcagg tcgataggca
    82561 gtaaatccat caggttcatc aggaataccc agacgaagat cgtcagggcc agcggagcaa
    82621 tcagcttgct tttgccatgg tacatgtctt tcacgctacc attaacaaag ccgatcacca
    82681 gctcaatcgc ggtctgaaac ttacctggca caccgctggt cgcctttttg gctacgctac
    82741 ggaataaaac caggaacaac agacccagca ccaccgagaa gaacatggag tcaatattga
    82801 ttgtccagaa ggtggctggg gggttttgtg gatccaccag cgagaatgta cgcaggtcca
    82861 gctgaaggtt attcaggtgg tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca
    82921 tgatgccttt taccctttgt tgttaattac agccggtgcc agtatctgaa ccaccagcac
    82981 caaaacccac gtaacgatca gcggcaagaa taccgccttt aaaaccgcca acgccaccac
    83041 cagtaacacc aacatcgcca gaactttgaa agcttcgcca aatgcgaatg tccaggccac
    83101 ccggcctttc gctggtgtat gcgcctggtg acgccaggca aatatcataa acaaaacgtt
    83161 aggcagaaag actgccaggc ccccgcttat tgcagagacg ccccagaagg ggtctttgag
    83221 gctgaacagc aatccacttg ctatcaccac cagtaactga acgagcagaa gcttccgagc
    83281 aacgtttcga ctcacgagcg acacagacat cacgtttttc actcctgctc ccttcgaggt
    83341 atgccgcgtg tcgtataaaa ctttctttaa ggcttagagt caagcatcaa aaagcggtca
    83401 aattatacgg tgcgcccccg tgatttcaaa caataagtag ccaaaaggtg aataaatgtt
    83461 taaatatttt tccagtgcat acaattgcga cttttctgct aaccctgttc gatcatgaaa
    83521 aactgtaaat aacgcgtaaa cactggtgat aaagcgtgct tcagatcaca tattgcgcat
    83581 gttcgcgcac agcatattta tttacttggc aaatgatgcc tttgcaagtt tatgatattt
    83641 aagtctaaaa acagatactg ttttaataaa tgacatttac acaacaaaaa ccacccattg
    83701 acatttttaa taatgtttta acagccaatg atgattctta gcgccgattt ttagcagact
    83761 gatattttca ctaatgactt attttctgct taccaaaaaa agccacgtta tcttgttgat
    83821 gcaaaagagt gaacgtggcg ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt
    83881 gttaacagtc taaccggtca attttttatg atttttttga taaaaattaa attttatttg
    83941 ctttaatcac caccagatgg cgttcgccat ccagggcagg aacctgaagt ttaaccactg
    84001 attcaacctg atattcttcg ggcaacaaag cgatttcatc ttccggcatt tgccctttca
    84061 gcgcgtagaa acggccttgc tcaccaggaa gatggtgaca ccagctcacc atatcgttca
    84121 gagaggcaaa agcgcggcta attacgccat caaatggcgg ctctgaagga aactcttcta
    84181 ccctgctctg tactggctca atattctcca gtttaagctc atgttgcacc tgacgaagga
    84241 aacgcacgcg tttaccaagg ctatccaaca gagtgaaatg ggcttcagga cgcacgatag
    84301 agagtggaat gccaggcagt ccaggaccgg tgccgacatc gataaaccgt tcaccttgca
    84361 gatacggtgc caccacaatg ctatcgagaa tatggcgtac cagcatctca ttaggatcgc
    84421 ggaccgaagt caggttgtac gctttgttcc atttatgcag catattcacg taggcaataa
    84481 gctggttttt ctggtgatcg gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt
    84541 tgttgagcac ggtgattacc tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg
    84601 catttttaat gcgttatgcg ctacgacgca gcataccctg ttttttcagc cacaccagca
    84661 gaatggagat ggccgcaggc gtgacgccag aaatacgtga agcctggccg atagaggccg
    84721 gtttgtgatc gttaagtttg gcgatcactt cgttagaaag accggatacc tggcggtaat
    84781 ccagtgtcgc tggtagcagg gtgttctcgt tacgcagctg cttttcgatc tcatcttgct
    84841 ggcgcgcgat ataaccttcg tatttaacct gaatctcaac ctgttccgcc gcctgttcgt
    84901 ctgtcaacgc aggggcaaac ggcgtcagcg tggttaattt ttcataagtc atttccggac
    84961 gacgcagcag atcttcacca ctggcttcac gggaaagcgg cgcagtcagg tgagcattca
    85021 cttcggctgc agcttccgcc gacggggtta cccaggtcga tttcagacgc tgacgctcac
    85081 gctcgatatt ctcaagtttc tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca
    85141 gttcacgacc gatttcagtc aaacgcagat ccgcattatc ttcgcgcagc atcagacgat
    85201 attctgcgcg cgaagtaaac atacgatacg gttctttggt tcctaaagtg cacaggtcat
    85261 caaccagtac gccgagatag gcctgagaac gcgccggagc ccaaccttct ttgtcagcag
    85321 acagacgggc agcgttaaga ccggccagca aaccttgcgc agcggcttct tcgtaaccgg
    85381 tagtgccgtt aatctgacca gcaaagaaca gcccctggat aaacttgctc tccagcgtcg
    85441 gtttcaggtc gcgaggatcg aagaagtcat actcaatggc ataacccgga cgcacgatct
    85501 tcgcgttttc catcccctgc atagagcgga cgatttgcat ctgcacatcg aacggcaggc
    85561 tggtggagat accgttcgga taaatttcgt tagaagtcag cccttccggt tcaaggaaga
    85621 tctgatgctg atttctgtcg gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc
    85681 gtgggccgac accttcaatc acccctgcat acattgggct acgatcgagg ttactgcgga
    85741 tcacatcatg ggttttctcg ttggtatgag tgatataaca cggcacctgc tggggatgct
    85801 gggacgcatt gcccataaac gagaataccg gcattgggtt atcgccatgc tgttgcgcca
    85861 gcacactaaa gtcgatagtt cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac
    85921 caacgcgcag cggcagttca cgcaaacggc gagaaagcgg aatggacggc ggatcaccag
    85981 cacggccacc gctgtaatta tccagaccga tatgaatttt accgtcgagg aacgtcccaa
    86041 cggtgagcac gacggctttg gcacggaact tcagtcccat ttgggtaaca gcaccgacca
    86101 cgcgatcgtt ttcgacaata agatcttcaa ccgcctgctg gaagatcatc aggttcggtt
    86161 ggttctccag cgccgtacgt accgcctgac ggtagagcac acgatccgcc tgagctcggg
    86221 tagcgcgaac cgccggtcct ttgcttgcgt ttagtatcct aaactggata cccgcttgat
    86281 cgatcgcttt cgccatcaga ccgccgagtg catccacttc ttttaccaga tgtcccttcc
    86341 caataccgcc gatcgccggg ttgcagctca tctgccccag agtgtcgata ttgtgtgtca
    86401 aaagcagagt ctgttgaccc atacgcgccg cggccatcgc ggcctcggtg cctgcatgac
    86461 ccccgccaat gatgatgacg tcaaaaggat ccggataaaa catggtgatt gcctcgcata
    86521 acgcggtatg aaaatggatt gaagcccggg ccgtggattc tactcaactt tgtcggcttg
    86581 agaaagacct gggatcctgg gtattaaaaa gaagatctat ttatttagag atctgttcta
    86641 ttgtgatctc ttattaggat tgcactgccc tgtggataac aaggatccgg cttttaagat
    86701 caacaacctg gaaaggatca ttaactgtga atgatcggtg atcctggacc gtataagctg
    86761 ggatcagaat gaggggttat acacaactca aaaactgaac aaccgttgtt ccttggataa
    86821 ctaccggttg atccaagctt cctgacagag ttatccacag tagatcgcac gatctgtata
    86881 cttatttgag taaattaatc cacgatccca gccattcttc tgccggatct tccggaatgt
    86941 cgtgatcaag aatgttgatc ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga
    87001 gttctgcctc gagtttatcg atagccccac aaaaggtgtc atattcacga ctaccaatac
    87061 cgattgcgcc aaagcggacc gcagaaagat cgggcttctg ttcctgcaat gcttcataga
    87121 aaggagaaag gttgtccgga atatctccgg caccgtgggt ggagctgata atcagccaga
    87181 tccccgaggc agataaatct tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg
    87241 cctcttccag cttttcagcc aggtgttctg ctacatattc ggcaccgccg agggtgctgc
    87301 cgctgataag agtgatatct gccataaacc gccaccttta ttaagagtgg cgtattgtac
    87361 gctgtgaacg cgttgggatc tacctgtgga aaagtatggg attaaaaaag ccgatcaggg
    87421 cttgatagta cgcatgatcg ggttttgcag gacgatcaat gtctcggtgg actgaatttc
    87481 atcaattgtt tggatcttgt tgataagtac atgctggaga gcgtcgatcg aacggcacat
    87541 tacttttata aagatgctgt agtggccggt tgtgtaatag gcttcagtga cttcatcaag
    87601 gctttccagc tttgccagcg cggaagggta gtctttggcg ctctttaata taatgccgat
    87661 aaagcagcct acgtcataac cgagctgctt cgggctgaca tcaatacgcg ccccggtaat
    87721 gatccccgcc tgcttcattt tctctactcg aacgtgaatc gtccccggac tgacgccaaa
    87781 ttgtttcgcc agttcggcgt aagcggtgcg cgcattgccc attaatgctt ccaggatgcc
    87841 acggtccaga ttgtcgatca gataattttc cataggattt tcttatgcag attgatgatt
    87901 cattctattt tagccttctt ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg
    87961 attattgcat gtgtgtcggt ttttgttgct taatcataag caacaggacg caggagtata
    88021 aaaaatgaaa accgcttaca ttgccaaaca acgccaaatt agcttcgtga aatctcactt
    88081 ttctcgccaa ctggaagaac gtctggggct gatcgaagtc caggcgccga ttcttagccg
    88141 tgtgggggat ggcacgcagg ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt
    88201 gaaagctctg cctgatgccc agttcgaagt ggttcattca ctggcgaagt ggaaacgtca
    88261 gaccttaggg caacacgact tcagcgcggg cgaagggctg tacacgcaca tgaaagccct
    88321 tcgccccgat gaagagcgtc tttctccgtt gcactcggtc tatgttgacc agtgggactg
    88381 ggaacgcgta atgggcgacg gtgagcgtca attctcgact ctgaaaagca cggtagaggc
    88441 gatctgggcg ggaattaaag caaccgaagc tgcagttagc gaagagtttg gtttggcacc
    88501 gttcctgccg gatcagatcc acttcgtaca cagccaggag ttactgtctc gttatccgga
    88561 tctcgatgcc aaagggcgtg agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt
    88621 cgggattggc ggcaagctga gtgatggtca tcgtcacgac gtgcgcgcac cggattatga
    88681 tgactggagc accccgtcag agctgggcca tgcgggtctg aacggcgata ttctggtatg
    88741 gaaccctgta ctggaagatg cgtttgagct ttcttccatg gggatccgcg tggatgccga
    88801 cacgttgaag catcagttgg cgctgaccgg tgatgaagat cgtctgcagc tggaatggca
    88861 tcaggcgctg ctgcgcggtg aaatgccgca gaccatcggc ggcggtatcg gccagtctcg
    88921 tttgaccatg ctgctgctgc aactgccgca tatcggccag gttcagtgtg gagtatggcc
    88981 agctgctgtt cgcgagagcg tcccttctct gctgtaataa tttatcgccg ccagcgtctg
    89041 agcaggcggc ttcgcatccc ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg
    89101 ccgggtttgc cgtgtgccga catcgccacg gcatgaaagc gatgctgatg tacccgctgc
    89161 agctctttca ctttactcgt cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc
    89221 accaccgcat cggcatcaaa ccattccctg ctttgcaagc gttccataat ggcgcgaaaa
    89281 caactggcaa gatcggtgcc gccacgaaac tgctggctta aaaaacggat tgcttgttcg
    89341 atgccttgtg ggcctgaaag ctcataacgg acgatctcgg tggaaaatag cataatatag
    89401 cagcgccggt tttctgcgag agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac
    89461 tgttcattaa agccgcccat tgagccggaa gtatccacac agacaataaa cggcccgcgc
    89521 ggctgttcat cgtaatcttt atgtaccacc ggacgttcga tcactttttc acgccacgac
    89581 tcaccgtgca ggcgataggt gagcaactgt ttttccacca gccgacggta aaactcatac
    89641 tccagttccg ttatccctag tgtcgccagt tctggcggca gaagacgtaa aatatcatcg
    89701 ctttgttgca gaccatcaac ctgctcagga accgtcgccg gttcgcgcac catggtgcgg
    89761 aaggtttcca tctgcgcatc gttgcgcggt attgatttgg cttcccgaga acgccccagc
    89821 tgctctgcca ggcgtttcag ttccggctgt tcgttaagaa attcaccgta tttcacaatc
    89881 aactgatagt cgccacgttt aagctgaccg gcgctcatat cccacagacg accagctgca
    89941 gtattgttat ctgcgagaat cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact
    90001 tcactcaaca gttgttcgcg ttcttcttct aatagctgtt gatttaacgt cgttgcttgc
    90061 acgatcagac ttaaacgcca acgctggaga aaaagcgtga gtaacgctga agtgatcgtg
    90121 ctgttagcat caaccaactg tcgggcttgt tctgcccatg gagaattcag acgatgcagt
    90181 aagtccagga tctgtggtag ctgcacaata aactgtggcg tggagaggag ctggctttgc
    90241 tgatagcaca tcacctcttc ggtgagttcc ggcgggactc gggcatcttt cagccgactg
    90301 cgcagcgctt cacgccagcg gggaacatca tcagtgattg ccgccttcag gcgtgggaat
    90361 ttttcaaaga agaccgccag ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc
    90421 aatccctctt cgctgacggc cagcatcaca ttaagcgtat ccagcgttag cattgttgtg
    90481 cctggcgaat ctgtgcaccg acatcctgta ggctggcttc aatgcgacct aaccagtcgc
    90541 cagggataaa cagacatttt tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc
    90601 tttcaagctc ttccagttgt tgttttattt caccaggcag accttcagcc gatgaaccgg
    90661 gaagtgccag cgtactgcct tgtaaactta catcgcgaac gacaagatgt tgggcgctat
    90721 caatttccag attcagtttc tgggcaaagc cgataccgtt cagtttaccg cgaatttcac
    90781 cacctttgct cagccactgt tccagcgcgc tacgctcaaa ggagatatga accacttcca
    90841 tatcatgcag ttttaacggt ttttgcagca gtagagtcag agtggaagca gtaacgttaa
    90901 caggaagttg atactgctga cgacggctga aaatgccgcc cagacgaatt accgttaagg
    90961 ctgttttatc gctttgttgc tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc
    91021 gagtcaacat cccttgctgt tgccaggcgt gaccggtcat caatacatca atttgttgtt
    91081 gtatcaaatt caggctttgc gcgtcatacc acaggcaatc tttcagcaaa atgagatcta
    91141 ccggggcaac agcactgcga ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt
    91201 ttttccagcg acgatccgag acataaggcg catccggtaa tttatccagt tgctggcgca
    91261 gcataaaaat cagctcaaat acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc
    91321 agcgttcata ttcttcatct gtgacctgca aggcatcagg aaccggattg tcgttttcat
    91381 cctgttgact ggtcagcatg gagcggaaat tcgctttatc ctgcacttta tctaaccaca
    91441 gacgaatcag catgcggtca tataacgctt ccagactgct gtctgcttcc ggcagctcgt
    91501 tggaggccgc caccagcaga cgcatcggga ttttttctac gtgtgcaccg ttgcggaact
    91561 ggcgctcgtt aatggcggtg agcaaggtat taagaattgc cgggcccgct ttccagatct
    91621 catccagaaa gacaatttct gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc
    91681 cttcatcttt tagcgcctga atagaaaggg gaccaaaaac ttcttccggc gtggagaagc
    91741 gggtcatcag atattcaaac gcgcgggcat tctgaaaggc gaattttaag cgccgggcga
    91801 tcaaactttt ggcaatacct ggcgggccaa ggaggaacac actttcacca cttaatgccg
    91861 ctaataaaca caagcggatg gcgtggctac gttcataaag ccccttttcc aacgaactgc
    91921 tcaggcggga aattctttcc gctaataaat gagggtgagc cataatgaag tggcgtcctt
    91981 tcgtcaaaag ttctgcgtaa attgcgagta tagacgtttc ttgctggtgg ctaaaatagt
    92041 ctcaaagggg gggtattttt ctttgagcca ggttaatgtg gccgcattta ggagtacgat
    92101 tttgccgtta atcgtgcata ctgtgcgctt ttttgtgggc caagggacta agcacacatt
    92161 tcatatttca acgaaagact agtctatgag cactgataat aagcaatcat tgcccgcgat
    92221 taccctcgcg gcgattggag ttgtctacgg cgatattggt accagcccgt tatatacact
    92281 tcgtgaatgt ttgtccggcc agtttggttt tggcgttgaa cgcgatgccg tgtttggctt
    92341 tttatcgctg atcttctggc tgctaatctt tgtggtttcc attaaatatc tcaccttcgt
    92401 gatgcgggca gataacgccg gtgagggggg gatcctgacg ttgatgtcgc ttgccgggcg
    92461 taatacgtcg gcgcgaacca catcaatgct ggtgattatg gggctaatcg gcggcagctt
    92521 tttctatggt gaagtcgtca taacacccgc tatttcggtg atgtcagcca ttgaaggtct
    92581 ggaaatcgtc gccccgcagc tggatacctg gatagttccc ctctcaatta tcgttctcac
    92641 tttattattt atgattcaaa aacatggcac ggctatggtc ggtaagctgt ttgcgccgat
    92701 catgctgacc tggtttttga ttctggcagg actggggtta cgtagcatta ttgctaaccc
    92761 ggaagtgctg catgcgctga atccaatgtg ggcggtgcat ttcttccttg aatacaaaac
    92821 ggtttctttt attgcattag gggcagtggt gttgtcgatt acgggggtcg aggcgctgta
    92881 tgctgatatg gggcactttg gtaagttccc tattcgcctg gcgtggttca ccgtcgtatt
    92941 accttccttg acccttaatt acttcggtca gggagcgctg ttgttaaaga acccggaagc
    93001 gattaagaac ccgttcttcc tgttggcacc ggactgggcg ctgatcccgc tgctgatcat
    93061 cgccgcactg gcgacggtaa ttgcctcgca ggcggttatc tctggcgtct tctcattgac
    93121 gcgtcaggcg gtacgtctgg gatatttgtc gccgatgcgc attattcaca cctccgaaat
    93181 ggagtcaggg caaatctata ttccgtttgt gaactggatg ctctatgtcg cggtcgtgat
    93241 tgtgattgtc agctttgagc actccagcaa cctggcggcg gcgtacggga ttgcggtgac
    93301 cggaaccatg gtgctgacgt ctattctctc gactaccgtg gcacgtcaga actggcactg
    93361 gaataagtat tttgttgcgc tgatcctgat tgctttcctt tgtgtcgata ttccattgtt
    93421 caccgctaac ctcgataaac tgctctccgg cggctggttg ccattgagcc tcggtactgt
    93481 gatgtttatc gtgatgacca cctggaagag cgagcgtttc cgcttgctgc ggcggatgca
    93541 tgaacatggt aactctctgg aagcgatgat tgcttcgctg gagaaatcac cgcctgttcg
    93601 cgtgcccggg accgcggtgt atatgtcgcg tgcaatcaac gtcattccct ttgcgctgat
    93661 gcataacctt aaacataaca aggtattgca tgagcgggtg attctgttaa ctctgcgtac
    93721 cgaagacgcg ccatatgtcc ataatgtccg tcgggtacag attgaacaac tgtcgcccac
    93781 tttctggcgc gtggtggcaa gttatggttg gcgagaaacg ccaaacgtgg aagaagtttt
    93841 ccaccgctgc ggtctggaag gattaagttg ccggatgatg gaaacctcct tctttatgtc
    93901 gcatgagtcg ttgatcctcg gcaaacgccc gtggtatttg cgtctgcgcg gcaagctgta
    93961 cttgctgctg caacgtaatg cgctgcgtgc accagatcaa tttgaaattc cgccaaacag
    94021 ggttatcgaa ctgggtactc aggtcgaaat ctaacgccag acgcctcctt tctccataag
    94081 ggggcgtttt tgttttcatg gttaatcacc atgtaaaacg tttcgaggtt gatcacattt
    94141 ccgtaacgtc acgatggttt tcccaactca gtcaggatta aactgcgggt cagcgaaacg
    94201 tttcgctgat ggagaaaaaa atgaaaaaag gcaccgttct taattctgat atttcatcgg
    94261 tgatctcccg tctgggacat accgatacgc tggtggtgtg tgatgctggt ttacccatcc
    94321 ccaaaagtac aacgcgtatc gatatggcat taacccaggg tgtaccttct tttatgcagg
    94381 tgctgggcgt cgtcacaaat gaaatgcagg tcgaggctgc cattatcgcg gaagagatca
    94441 aacaacataa tccgcaactc cacgaaacgt tgctcactca ccttgagcag ctgcaaaaac
    94501 accagggaaa taccattgaa attcgttaca ccacgcatga acaattcaaa caacaaaccg
    94561 cagaaagtca ggcggtaatt cgcagcggag aatgttctcc gtatgcgaat atcattctct
    94621 gtgctggcgt gacgttctga ggccgtcatg gaagcattac ttcagcttaa aggcatcgat
    94681 aaagccttcc cgggcgtaaa agccctctcg ggcgcagcgt taaatgtcta tccgggccgc
    94741 gtgatggcgc tggtgggcga aaacggcgcg ggtaaatcca ccatgatgaa agtgcttact
    94801 ggtatctatg ctcgcgatgc cggcacgctt ttatggctgg ggaaagaaac gacatttacc
    94861 gggccgaaat cttcccagga agccgggatt gggattatcc atcaggaact gaacctgatc
    94921 ccgcagttga ccattgccga aaacattttc ctcggtcgtg agtttgttaa tcgctttggc
    94981 aaaattgact ggaaaaccat gtatgccgaa gcggataaat tgctggctaa acttaacctg
    95041 cgctttaaaa gcgacaagct ggtgggcgat ctttccatcg gtgaccagca aatggttgaa
    95101 atcgccaaag tgctgagctt tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg
    95161 ctgaccgata ccgaaaccga atccctgttc cgcgtcatac gcgagctgaa atcgcaaggc
    95221 cgcggtattg tctatatctc ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt
    95281 accgtttttc gtgacgggca atttattgct gagcgcgaag tggcatcact gaccgaagat
    95341 tcgctgattg agatgatggt gggccgcaag ctggaagatc aatatccaca cctggacaaa
    95401 gcgccgggag atatccgcct gaaagtcgat aatctctgcg gacttggcgt taacgatgtc
    95461 tcttttactt tacgcaaagg cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt
    95521 accgaactga tgaaagtgct ctacggcgca ctaccgcgca ccagcggtta cgtcaccctg
    95581 gatgggcatg aagtcgttac ccgttcaccg caggatggcc tggcaagcgg cattgtgtat
    95641 atctccgaag accgtaaacg tgacggttta gtgttgggca tgtcagtaaa agagaacatg
    95701 tcgctgacag cgctgcgcta cttcagccgc gctggcggca gtttgaagca tgccgatgaa
    95761 cagcaggctg tgagtgattt cattcgtctg tttaatgtga aaacaccgtc gatggaacag
    95821 gcaattggtc tgctttccgg tggcaatcag caaaaagtgg cgattgcccg cggtctgatg
    95881 acacgcccca aagtgttgat ccttgatgaa cctacccgtg gcgtagatgt cggcgcgaaa
    95941 aaagagatct atcaactgat taaccagttc aaagccgatg gcttgagcat cattctggtg
    96001 tcatcggaga tgccagaagt attaggcatg agcgatcgca tcatcgtcat gcatgaaggg
    96061 catctcagcg gggaatttac tcgtgagcag gccacccagg aagtgttaat ggctgccgct
    96121 gtgggcaagc ttaatcgcgt gaatcaggag taaaaaaatg acaacccaga ctgtctctgg
    96181 tcgccgttat ttcacgaaag cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt
    96241 gctgatcgcg attgtctcga cgttaagccc gaactttttc accatcaata acttattcaa
    96301 tattctccag caaacctcgg tgaacgccat tatggcggtc gggatgacgc tggtgatcct
    96361 gacgtcgggc atcgacttgt ctgtaggttc tctgttggcg ctaaccggcg cagttgctgc
    96421 atctatcgtc ggcattgaag tcaatgcgct ggtggctgtc gctgctgctc tcgcgttagg
    96481 tgccgcaatt ggtgcggtaa ccggggtgat tgtagcgaaa ggtcgcgttc aggcgtttat
    96541 cgctacgctg gttatgatgc ttttactgcg cggcgtgacc atggtttata ccaacggtag
    96601 cccagtgaat accggcttta ctgagaacgc cgatctgttt ggctggtttg gtattggtcg
    96661 tccgctgggc gtaccgacgc cagtctggat catggggatt gtcttcctcg cggcctggta
    96721 catgctgcat cacacgcgtc tggggcgtta catctacgcg ctgggcggca acgaagcagc
    96781 aacgcgtctt tctggtatca acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg
    96841 tctgctggca tcgctggccg ggatcattga agtggcgcgt ctctcctccg cacaacccac
    96901 ggcggggact ggctatgagc tggatgctat tgctgcggtg gttctgggcg gtacgagtct
    96961 ggcgggcgga aaaggtcgca ttgttgggac gttgatcggc gcattaattc ttggcttcct
    97021 taataatgga ttgaatttgt taggtgtttc ctcctattac cagatgatcg tcaaagcggt
    97081 ggtgattttg ctggcggtgc tggtagacaa caaaaagcag taataacgac tacaggacat
    97141 cttgaatatg aacatgaaaa aactggctac cctggtttcc gctgttgcgc taagcgccac
    97201 cgtcagtgcg aatgcgatgg caaaagacac catcgcgctg gtggtctcca cgcttaacaa
    97261 cccgttcttt gtatcgctga aagatggcgc gcagaaagag gcggataagc ttggctataa
    97321 cctggtggtg ctggactccc agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt
    97381 aaccgttcgc ggcaccaaaa ttctgctgat taacccgacc gactccgacg cagtaggtaa
    97441 tgctgtgaag atggctaacc aggcgaacat cccggttatc actcttgacc gccaggcaac
    97501 gaaaggtgaa gtggtgagcc acattgcttc tgataacgta ctgggtggca aaatcgccgg
    97561 tgattacatc gcgaagaaag cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc
    97621 tggtacatcc gcagcccgtg aacgtggcga aggcttccag caggccgttg ctgctcacaa
    97681 gtttaatgtt cttgccagcc agccagcaga ttttgatcgc actaaaggtt tgaacgtaat
    97741 gcagaacctg ttgaccgctc atccggatgt tcaggctgta ttcgcgcaga atgatgaaat
    97801 ggcgctgggc gcgctgcgcg cactgcaaac tgccggtaaa tcggatgtga tggtcgtcgg
    97861 atttgacggt acaccggatg gcgaaaaagc ggtgaatgat ggcaaactag cagcgactat
    97921 cgctcagcta cccgatcaga ttggcgcgaa aggcgtcgaa accgcagata aagtgctgaa
    97981 aggcgagaaa gttcaggcta agtatccggt tgatctgaaa ctggttgtta agcagtagtt
    98041 ttaatcaggt tgtatgacct gatggtgaca taaatacgtc atcgacagat gaacgtgtaa
    98101 tataaagaaa agcagggcac gcgccaccct aacacggtgg cgcattttat ggacatcccg
    98161 aatatgcaaa acgcaggcag cctcgttgtt cttggcagca ttaatgctga ccacattctt
    98221 aatcttcaat cttttcctac tccaggcgaa accgtaaccg gtaaccacta tcaggttgca
    98281 tttggcggca aaggcgcgaa tcaggctgtg gctgctgggc gtagcggtgc gaatatcgcg
    98341 tttattgcct gtacgggtga tgacagcatt ggtgagagcg ttcgccagca gctcgccact
    98401 gataacattg atattactcc ggtcagcgtg atcaaaggcg aatcaacagg tgtggcgctg
    98461 atttttgtta atggcgaagg tgagaatgtc atcggtattc atgccggcgc taatgctgcc
    98521 cttaccccgg cgctggtgga agcgcaacgt gagcgtattg ccaacgcgtc agcattatta
    98581 atgcagctgg aatcaccact cgaaagtgtg atggcagcgg cgaaaatcgc ccatcaaaat
    98641 aagactatcg ttgcgcttaa cccggctccg gctcgcgaac ttcctgacga actgctggcg
    98701 ctggtggaca ttattacgcc aaacgaaacg gaagcagaaa agctcaccgg tattcgtgtt
    98761 gaaaatgatg aagatgcagc gaaggcggcg caggtactgc atgaaaaagg tatccgtact
    98821 gtactgatta ctttaggaag tcgtggtgta tgggctagcg tgaatggtga aggtcagcgc
    98881 gttcctggat tccgggtgca ggctgtcgat accattgctg ccggagatac ctttaacggc
    98941 gcgttaatca cggcgttgct ggaagaaaaa ccattgccag aggcgattcg gtttgcccat
    99001 gctgccgctg cgattgccgt aacacgtaaa ggcgcacaac cttccgtacc gtggcgtgaa
    99061 gagatcgacg catttttaga caggcagagg tgacgcttgg ctacaatgaa agatgttgcc
    99121 cgcctggcgg gcgtttctac ctcaacagtt tctcacgtta tcaataaaga tcgcttcgtc
    99181 agtgaagcga ttaccgccaa agttgaagcg gcgattaaag aactcaatta cgcgccatca
    99241 gccctggcgc gtagcctcaa actcaatcaa acacatacca ttggcatgtt gatcactgcc
    99301 agtaccaatc ctttctattc agaactggtg cgtggcgttg aacgcagctg cttcgaacgc
    99361 ggttatagtc tcgtcctttg caataccgaa ggcgatgaac agcggatgaa tcgcaatctg
    99421 gaaacgctga tgcaaaaacg cgttgatggc ttgctgttac tgtgcaccga aacgcatcaa
    99481 ccttcgcgtg aaatcatgca acgttatccg acagtgccta ctgtgatgat ggactgggct
    99541 ccgttcgatg gcgacagcga tcttattcag gataactcgt tgctgggcgg agacttagca
    99601 acgcaatatc tgatcgataa aggtcatacc cgtatcgcct gtattaccgg tccgctggat
    99661 aaaactccgg cgcgcctgcg gttggaaggt tatcgggcgg cgatgaaacg tgcgggtctc
    99721 aacattcctg atggctatga agtcactggt gattttgaat ttaacggcgg gtttgacgcc
    99781 atgcgccaac tgttatcaca tccgctgcgt cctcaggccg tctttaccgg aaatgacgct
    99841 atggctgttg gcgtttacca ggcgttatat caggcagagt tacaggttcc gcaggatatc
    99901 gcggtgattg gctatgacga tatcgaactg gcaagcttta tgacgccacc attaaccact
    99961 atccaccaac cgaaagatga actgggggag ctggcgattg atgtactcat ccatcggata
   100021 acccagccga cccttcagca acaacgatta caacttactc cgattctgat ggaacgcggt
   100081 tcggcttaga tttacgctgt cttttgatca aattattacc atcggttgtt ttcagaagca
   100141 tgaacattgc tgctgaagca acagtaataa tgcccatcgt gataaacgta tagtggaatt
   100201 gttcgacagt cgttgtgcct tccatccctt cataaacgcg aaggacggcc gcacttacag
   100261 caacgcctaa actaatcgac agttgctgcg tgaccgccag aacactgtta ccgctgctgg
   100321 cgttgtcatc ggtcagatcg gcaagtgtga tggtattcat cgcggtaaat tgcgtcgaca
   100381 tagccatccc taatataaac aacggcaaga tcagcatcca tatcgccatt gccggtgatt
   100441 gcaaagagaa ctgagcgatc attagcccaa taatcaccgt gatccccact aacgtatggc
   100501 gatagcccag acgacgtaag acttgggtaa ccatcgattt tgcaataatg gaacctaacg
   100561 ctgtcggtgc catcatacag ccggcaataa acgcctgata accaaatcct acctgtaaca
   100621 tcaatggcat aaggaacggt acacaaccgg tccccagacg ggttgcaata ttgcctacga
   100681 taccgatcga gaaagtgcgg gttttaaata aatctaatga aattaatggg tttggcgtgt
   100741 gtcgcgcatg gagaatatag agaagcagta acccgatgct ggtgacaatt accgtcaagg
   100801 caatccagct ggcgacaatc ctttccccga atagctctat tccgcttgag aagagaacaa
   100861 ggctgaggcc aaacagcaaa aagccagtga tatcgaatct gcgtcgtgcg gtggtgaaat
   100921 tgggcatatg tttgcgcgcg taaagaaggc ccgcaatacc tatggggata ttgattaaaa
   100981 atatccagtg ccaggttgcc caggtgacca gcacgccgcc aagaacgggg cctaaaattg
   101041 gccccaccag acccggcatg gcgacaaaat tcaatactgg aagaagttca ttacgaggat
   101101 aagcgcgcag taaggccagc cgagcaacag gcatcatcat tgcgccgcct atcccctgaa
   101161 taacccggaa gacaaccagc tgtggtagcg aattagaaag tgcgcaggcc agagaaccca
   101221 atgtgaacag actcacggca agggtaaaaa tgcgacgcgt accgaagcga tcggctagcc
   101281 atccgcttac cggaataagc atcgccaccg tcagcgtata actgatgatg gctgattgca
   101341 tcgcgagagg agaacgatta aggctatgag cgattgcggg taaggcggta ttcagaatag
   101401 tggcatcaag tgcctgcatg aagaaggcca tcgccgcgat ccacggcaaa cccgccatac
   101461 tgcgcttctt tttatcgctc attcaatgtc ctgttatcgg gttgtcactt atcaggtgag
   101521 cgtagcagcg cctgacaagc tttaaatgcc gcgtcgccat cgctttggac aatcgcatcg
   101581 acaatcgcct ggtgcagatc cagctttatc actgtgtcgc tggtaattga cgtgaagtaa
   101641 gtgtgataaa ccgaatggaa tagcgaggcg aatgaggtca aaaacggatt ggcgctcatt
   101701 tcatagatat gctcatgcca ggccatatcg acttcgatcc agcgttcacg gcgaaagttc
   101761 tcttttaatg ccgccatttc ggccattaac gtattgagat gcgccttctg ttccgcggtg
   101821 ccaaccgttg cagccagtag gcaagcttgc ggctccagac agatacgcat aaccagaaag
   101881 tgatcgatga cctgatgaaa gttctcttct gtcatccacc aggtaagcaa ttcctgatca
   101941 agaaaattcc agtttgattg tggcatgacc cgggtaccaa ttcgcggtcg cggtaaaacc
   102001 atcccttttg ccgttaacgt tttgaccgct tcgcgtaccg ctgtacgact cactccaaat
   102061 tgctcgccca gctcaatctc accaggcaaa atggtgccgg gttcatattc accttttaag
   102121 atgcgttgcg ccagcttctc agccagaaca tacgaaaggt ttttctgtgc agctaactgt
   102181 tgtgcgctta aaggcattac taatcttcct ttttcttttt attcctcctt agtatgccac
   102241 caggaagtgt gattacggtt gcaaaaacgg caaattgctt gttttatggc acattaacgg
   102301 ggcttttgct gaaaaaatgc gcggtcagaa aattatttta aatttcctct tgtcaggccg
   102361 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gccgccgggt
   102421 cagcggggtt ctcctgagaa ttccgacaga gaaagcaaaa ataaatgctt gactctgtag
   102481 cgggaaagcg tattatacac accccgcgcc gctgagaaaa agcgaagcgg cactgctctt
   102541 taacaattta tcagacaatc tgtgtgggc
//
LOCUS       DAZFYC010000005        95170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFYC010000005 DAZFYC010000000
VERSION     DAZFYC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.005"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            108..1364
                     /gene="ydhQ"
                     /locus_tag="ACHKW5_000431"
     CDS             108..1364
                     /gene="ydhQ"
                     /locus_tag="ACHKW5_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7509878.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(1405..2778)
                     /gene="mdtK"
                     /locus_tag="ACHKW5_000432"
     CDS             complement(1405..2778)
                     /gene="mdtK"
                     /locus_tag="ACHKW5_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO7509879.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            2993..3634
                     /gene="ribE"
                     /locus_tag="ACHKW5_000433"
     CDS             2993..3634
                     /gene="ribE"
                     /locus_tag="ACHKW5_000433"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO7509880.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(3674..4822)
                     /gene="cfa"
                     /locus_tag="ACHKW5_000434"
     CDS             complement(3674..4822)
                     /gene="cfa"
                     /locus_tag="ACHKW5_000434"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO7509881.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(5113..6324)
                     /gene="punC"
                     /locus_tag="ACHKW5_000435"
     CDS             complement(5113..6324)
                     /gene="punC"
                     /locus_tag="ACHKW5_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO7509882.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            6437..7369
                     /gene="punR"
                     /locus_tag="ACHKW5_000436"
     CDS             6437..7369
                     /gene="punR"
                     /locus_tag="ACHKW5_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO7509883.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMNGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(7366..8391)
                     /gene="purR"
                     /locus_tag="ACHKW5_000437"
     CDS             complement(7366..8391)
                     /gene="purR"
                     /locus_tag="ACHKW5_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO7509884.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            8690..8779
                     /gene="cydH"
                     /locus_tag="ACHKW5_000438"
                     /gene_synonym="ynhF"
     CDS             8690..8779
                     /gene="cydH"
                     /locus_tag="ACHKW5_000438"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO7509885.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            8945..10114
                     /gene="ydhP"
                     /locus_tag="ACHKW5_000439"
     CDS             8945..10114
                     /gene="ydhP"
                     /locus_tag="ACHKW5_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7509886.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(10260..10841)
                     /gene="sodB"
                     /locus_tag="ACHKW5_000440"
     CDS             complement(10260..10841)
                     /gene="sodB"
                     /locus_tag="ACHKW5_000440"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO7509887.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(10969..11784)
                     /gene="mepH"
                     /locus_tag="ACHKW5_000441"
     CDS             complement(10969..11784)
                     /gene="mepH"
                     /locus_tag="ACHKW5_000441"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO7509888.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTTSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            12119..12466
                     /gene="grxD"
                     /locus_tag="ACHKW5_000442"
     CDS             12119..12466
                     /gene="grxD"
                     /locus_tag="ACHKW5_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO7509889.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(12517..17133)
                     /gene="lhr"
                     /locus_tag="ACHKW5_000443"
     CDS             complement(12517..17133)
                     /gene="lhr"
                     /locus_tag="ACHKW5_000443"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO7509890.1"
                     /translation="MADNPDSSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHQARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(17226..17873)
                     /gene="rnt"
                     /locus_tag="ACHKW5_000444"
     CDS             complement(17226..17873)
                     /gene="rnt"
                     /locus_tag="ACHKW5_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO7509891.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(17976..18383)
                     /gene="gloA"
                     /locus_tag="ACHKW5_000445"
     CDS             complement(17976..18383)
                     /gene="gloA"
                     /locus_tag="ACHKW5_000445"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO7509892.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(18464..19561)
                     /gene="nemA"
                     /locus_tag="ACHKW5_000446"
     CDS             complement(18464..19561)
                     /gene="nemA"
                     /locus_tag="ACHKW5_000446"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO7509893.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(19598..20197)
                     /gene="nemR"
                     /locus_tag="ACHKW5_000447"
     CDS             complement(19598..20197)
                     /gene="nemR"
                     /locus_tag="ACHKW5_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO7509894.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            20300..20539
                     /gene="ydhL"
                     /locus_tag="ACHKW5_000448"
     CDS             20300..20539
                     /gene="ydhL"
                     /locus_tag="ACHKW5_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO7509895.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            20588..21484
                     /gene="ydhF"
                     /locus_tag="ACHKW5_000449"
     CDS             20588..21484
                     /gene="ydhF"
                     /locus_tag="ACHKW5_000449"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO7509896.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            21565..22086
                     /gene="sodC"
                     /locus_tag="ACHKW5_000450"
     CDS             21565..22086
                     /gene="sodC"
                     /locus_tag="ACHKW5_000450"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7509897.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(22087..24099)
                     /gene="ydhK"
                     /locus_tag="ACHKW5_000451"
     CDS             complement(22087..24099)
                     /gene="ydhK"
                     /locus_tag="ACHKW5_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7509898.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDVGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKSQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     IQRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(24099..24956)
                     /gene="ydhJ"
                     /locus_tag="ACHKW5_000452"
     CDS             complement(24099..24956)
                     /gene="ydhJ"
                     /locus_tag="ACHKW5_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7509899.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(24959..25195)
                     /gene="ydhI"
                     /locus_tag="ACHKW5_000453"
     CDS             complement(24959..25195)
                     /gene="ydhI"
                     /locus_tag="ACHKW5_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO7509900.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            25396..25830
                     /gene="slyA"
                     /locus_tag="ACHKW5_000454"
     CDS             25396..25830
                     /gene="slyA"
                     /locus_tag="ACHKW5_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO7509901.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(25877..26344)
                     /gene="slyB"
                     /locus_tag="ACHKW5_000455"
     CDS             complement(25877..26344)
                     /gene="slyB"
                     /locus_tag="ACHKW5_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO7509902.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            26618..27727
                     /gene="anmK"
                     /locus_tag="ACHKW5_000456"
     CDS             26618..27727
                     /gene="anmK"
                     /locus_tag="ACHKW5_000456"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO7509903.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            27825..28154
                     /gene="mliC"
                     /locus_tag="ACHKW5_000457"
     CDS             27825..28154
                     /gene="mliC"
                     /locus_tag="ACHKW5_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO7509904.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            28213..28869
                     /gene="pdxH"
                     /locus_tag="ACHKW5_000458"
     CDS             28213..28869
                     /gene="pdxH"
                     /locus_tag="ACHKW5_000458"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO7509905.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQLENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            28998..30272
                     /gene="tyrS"
                     /locus_tag="ACHKW5_000459"
     CDS             28998..30272
                     /gene="tyrS"
                     /locus_tag="ACHKW5_000459"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO7509906.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            30331..31194
                     /gene="pdxY"
                     /locus_tag="ACHKW5_000460"
     CDS             30331..31194
                     /gene="pdxY"
                     /locus_tag="ACHKW5_000460"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO7509907.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(31238..31843)
                     /gene="gstA"
                     /locus_tag="ACHKW5_000461"
     CDS             complement(31238..31843)
                     /gene="gstA"
                     /locus_tag="ACHKW5_000461"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO7509908.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPAVLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(31949..33451)
                     /gene="dtpA"
                     /locus_tag="ACHKW5_000462"
     CDS             complement(31949..33451)
                     /gene="dtpA"
                     /locus_tag="ACHKW5_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO7509909.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(34062..34697)
                     /gene="nth"
                     /locus_tag="ACHKW5_000463"
     CDS             complement(34062..34697)
                     /gene="nth"
                     /locus_tag="ACHKW5_000463"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO7509910.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(34697..35392)
                     /gene="rsxE"
                     /locus_tag="ACHKW5_000464"
     CDS             complement(34697..35392)
                     /gene="rsxE"
                     /locus_tag="ACHKW5_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO7509911.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(35396..36016)
                     /gene="rsxG"
                     /locus_tag="ACHKW5_000465"
     CDS             complement(35396..36016)
                     /gene="rsxG"
                     /locus_tag="ACHKW5_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO7509912.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(36020..37078)
                     /gene="rsxD"
                     /locus_tag="ACHKW5_000466"
     CDS             complement(36020..37078)
                     /gene="rsxD"
                     /locus_tag="ACHKW5_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO7509913.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(37079..39301)
                     /gene="rsxC"
                     /locus_tag="ACHKW5_000467"
     CDS             complement(37079..39301)
                     /gene="rsxC"
                     /locus_tag="ACHKW5_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO7509914.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQSNAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(39294..39872)
                     /gene="rsxB"
                     /locus_tag="ACHKW5_000468"
     CDS             complement(39294..39872)
                     /gene="rsxB"
                     /locus_tag="ACHKW5_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO7509915.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(39872..40453)
                     /gene="rsxA"
                     /locus_tag="ACHKW5_000469"
     CDS             complement(39872..40453)
                     /gene="rsxA"
                     /locus_tag="ACHKW5_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO7509916.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(40530..40970)
                     /gene="ydgK"
                     /locus_tag="ACHKW5_000470"
     CDS             complement(40530..40970)
                     /gene="ydgK"
                     /locus_tag="ACHKW5_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO7509917.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(41056..41271)
                     /gene="ydgT"
                     /locus_tag="ACHKW5_000471"
     CDS             complement(41056..41271)
                     /gene="ydgT"
                     /locus_tag="ACHKW5_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO7509918.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(41544..41669)
                     /gene="blr"
                     /locus_tag="ACHKW5_000472"
     CDS             complement(41544..41669)
                     /gene="blr"
                     /locus_tag="ACHKW5_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO7509919.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            41912..42952
                     /gene="ydgJ"
                     /locus_tag="ACHKW5_000473"
     CDS             41912..42952
                     /gene="ydgJ"
                     /locus_tag="ACHKW5_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7509920.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(42987..43988)
                     /gene="add"
                     /locus_tag="ACHKW5_000474"
     CDS             complement(42987..43988)
                     /gene="add"
                     /locus_tag="ACHKW5_000474"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO7509921.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(44092..45264)
                     /gene="malY"
                     /locus_tag="ACHKW5_000475"
     CDS             complement(44092..45264)
                     /gene="malY"
                     /locus_tag="ACHKW5_000475"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO7509922.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(45274..46866)
                     /gene="malX"
                     /locus_tag="ACHKW5_000476"
     CDS             complement(45274..46866)
                     /gene="malX"
                     /locus_tag="ACHKW5_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO7509923.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            47041..48069
                     /gene="malI"
                     /locus_tag="ACHKW5_000477"
     CDS             47041..48069
                     /gene="malI"
                     /locus_tag="ACHKW5_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO7509924.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            48181..48948
                     /gene="hdhA"
                     /locus_tag="ACHKW5_000478"
     CDS             48181..48948
                     /gene="hdhA"
                     /locus_tag="ACHKW5_000478"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO7509925.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            49177..49767
                     /gene="uidR"
                     /locus_tag="ACHKW5_000479"
     CDS             49177..49767
                     /gene="uidR"
                     /locus_tag="ACHKW5_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO7509926.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTVTEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            50155..51966
                     /gene="uidA"
                     /locus_tag="ACHKW5_000480"
     CDS             50155..51966
                     /gene="uidA"
                     /locus_tag="ACHKW5_000480"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO7509927.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            51963..53336
                     /gene="uidB"
                     /locus_tag="ACHKW5_000481"
     CDS             51963..53336
                     /gene="uidB"
                     /locus_tag="ACHKW5_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO7509928.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSFATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            53375..54640
                     /gene="uidC"
                     /locus_tag="ACHKW5_000482"
     CDS             53375..54640
                     /gene="uidC"
                     /locus_tag="ACHKW5_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO7509929.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAVQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLIFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(54685..56193)
                     /gene="ydgA"
                     /locus_tag="ACHKW5_000483"
     CDS             complement(54685..56193)
                     /gene="ydgA"
                     /locus_tag="ACHKW5_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7509930.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLESMEINGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMLALNVPVVPAIPQQ"
     gene            complement(56294..57469)
                     /gene="manA"
                     /locus_tag="ACHKW5_000484"
     CDS             complement(56294..57469)
                     /gene="manA"
                     /locus_tag="ACHKW5_000484"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO7509931.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            57668..59314
                     /gene="fumB"
                     /locus_tag="ACHKW5_000485"
     CDS             57668..59314
                     /gene="fumB"
                     /locus_tag="ACHKW5_000485"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7509932.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            59457..60860
                     /gene="fumC"
                     /locus_tag="ACHKW5_000486"
     CDS             59457..60860
                     /gene="fumC"
                     /locus_tag="ACHKW5_000486"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO7509933.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(60857..61786)
                     /gene="tus"
                     /locus_tag="ACHKW5_000487"
     CDS             complement(60857..61786)
                     /gene="tus"
                     /locus_tag="ACHKW5_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO7509934.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(61862..63163)
                     /gene="rstB"
                     /locus_tag="ACHKW5_000488"
     CDS             complement(61862..63163)
                     /gene="rstB"
                     /locus_tag="ACHKW5_000488"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO7509935.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(63167..63886)
                     /gene="rstA"
                     /locus_tag="ACHKW5_000489"
     CDS             complement(63167..63886)
                     /gene="rstA"
                     /locus_tag="ACHKW5_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO7509936.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            64015..64350
                     /gene="ydgC"
                     /locus_tag="ACHKW5_000490"
     CDS             64015..64350
                     /gene="ydgC"
                     /locus_tag="ACHKW5_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO7509937.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(64347..65069)
                     /gene="folM"
                     /locus_tag="ACHKW5_000491"
     CDS             complement(64347..65069)
                     /gene="folM"
                     /locus_tag="ACHKW5_000491"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO7509938.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(65106..66488)
                     /gene="ydgI"
                     /locus_tag="ACHKW5_000492"
     CDS             complement(65106..66488)
                     /gene="ydgI"
                     /locus_tag="ACHKW5_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7509939.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(66674..67618)
                     /gene="ydgH"
                     /locus_tag="ACHKW5_000493"
     CDS             complement(66674..67618)
                     /gene="ydgH"
                     /locus_tag="ACHKW5_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO7509940.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            68142..69674
                     /gene="pntA"
                     /locus_tag="ACHKW5_000494"
     CDS             68142..69674
                     /gene="pntA"
                     /locus_tag="ACHKW5_000494"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO7509941.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKTAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            69685..71073
                     /gene="pntB"
                     /locus_tag="ACHKW5_000495"
     CDS             69685..71073
                     /gene="pntB"
                     /locus_tag="ACHKW5_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO7509942.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(71098..72132)
                     /gene="tqsA"
                     /locus_tag="ACHKW5_000496"
     CDS             complement(71098..72132)
                     /gene="tqsA"
                     /locus_tag="ACHKW5_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO7509943.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLMAFAFNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            72544..72909
                     /gene="mdtJ"
                     /locus_tag="ACHKW5_000497"
     CDS             72544..72909
                     /gene="mdtJ"
                     /locus_tag="ACHKW5_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO7509944.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVMLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            72896..73225
                     /gene="mdtI"
                     /locus_tag="ACHKW5_000498"
     CDS             72896..73225
                     /gene="mdtI"
                     /locus_tag="ACHKW5_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO7509945.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(73264..74085)
                     /gene="ydgD"
                     /locus_tag="ACHKW5_000499"
     CDS             complement(73264..74085)
                     /gene="ydgD"
                     /locus_tag="ACHKW5_000499"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO7509946.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(74185..74268)
                     /locus_tag="ACHKW5_000500"
     CDS             complement(74185..74268)
                     /locus_tag="ACHKW5_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7509947.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(74361..74669)
                     /gene="asr"
                     /locus_tag="ACHKW5_000501"
     CDS             complement(74361..74669)
                     /gene="asr"
                     /locus_tag="ACHKW5_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO7509948.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETANTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            74940..75044
                     /locus_tag="ACHKW5_000502"
     CDS             74940..75044
                     /locus_tag="ACHKW5_000502"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO7509949.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(75093..76346)
                     /gene="ynfM"
                     /locus_tag="ACHKW5_000503"
     CDS             complement(75093..76346)
                     /gene="ynfM"
                     /locus_tag="ACHKW5_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7509950.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            76453..77346
                     /gene="ynfL"
                     /locus_tag="ACHKW5_000504"
     CDS             76453..77346
                     /gene="ynfL"
                     /locus_tag="ACHKW5_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7509951.1"
                     /translation="MNIELRHLRYFVAIAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYEDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            77481..78701
                     /gene="mlc"
                     /locus_tag="ACHKW5_000505"
                     /gene_synonym="dgsA"
     CDS             77481..78701
                     /gene="mlc"
                     /locus_tag="ACHKW5_000505"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO7509952.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLLGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            78826..79521
                     /gene="bioD"
                     /locus_tag="ACHKW5_000506"
     CDS             78826..79521
                     /gene="bioD"
                     /locus_tag="ACHKW5_000506"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7509953.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(79474..80766)
                     /gene="clcB"
                     /locus_tag="ACHKW5_000507"
     CDS             complement(79474..80766)
                     /gene="clcB"
                     /locus_tag="ACHKW5_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO7509954.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(80925..81539)
                     /gene="dmsD"
                     /locus_tag="ACHKW5_000508"
     CDS             complement(80925..81539)
                     /gene="dmsD"
                     /locus_tag="ACHKW5_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO7509955.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(81582..82436)
                     /gene="ynfH"
                     /locus_tag="ACHKW5_000509"
     CDS             complement(81582..82436)
                     /gene="ynfH"
                     /locus_tag="ACHKW5_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO7509956.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(82438..83055)
                     /gene="ynfG"
                     /locus_tag="ACHKW5_000510"
     CDS             complement(82438..83055)
                     /gene="ynfG"
                     /locus_tag="ACHKW5_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO7509957.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(83066..85489)
                     /gene="ynfF"
                     /locus_tag="ACHKW5_000511"
     CDS             complement(83066..85489)
                     /gene="ynfF"
                     /locus_tag="ACHKW5_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO7509958.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(85550..87976)
                     /gene="ynfE"
                     /locus_tag="ACHKW5_000512"
     CDS             complement(85550..87976)
                     /gene="ynfE"
                     /locus_tag="ACHKW5_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO7509959.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTTAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(88175..88480)
                     /gene="ynfD"
                     /locus_tag="ACHKW5_000513"
     CDS             complement(88175..88480)
                     /gene="ynfD"
                     /locus_tag="ACHKW5_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO7509960.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            88588..89298
                     /gene="ynfC"
                     /locus_tag="ACHKW5_000514"
     CDS             88588..89298
                     /gene="ynfC"
                     /locus_tag="ACHKW5_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO7509961.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VNPAVERVYTIKNTIDYY"
     gene            complement(89301..89861)
                     /gene="speG"
                     /locus_tag="ACHKW5_000515"
     CDS             complement(89301..89861)
                     /gene="speG"
                     /locus_tag="ACHKW5_000515"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO7509962.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(89896..90237)
                     /gene="ynfB"
                     /locus_tag="ACHKW5_000516"
     CDS             complement(89896..90237)
                     /gene="ynfB"
                     /locus_tag="ACHKW5_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO7509963.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            90372..90698
                     /gene="ynfA"
                     /locus_tag="ACHKW5_000517"
     CDS             90372..90698
                     /gene="ynfA"
                     /locus_tag="ACHKW5_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO7509964.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            90904..92118
                     /gene="rspA"
                     /locus_tag="ACHKW5_000518"
     CDS             90904..92118
                     /gene="rspA"
                     /locus_tag="ACHKW5_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO7509965.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            92130..93149
                     /gene="rspB"
                     /locus_tag="ACHKW5_000519"
     CDS             92130..93149
                     /gene="rspB"
                     /locus_tag="ACHKW5_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO7509966.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(93337..94632)
                     /locus_tag="ACHKW5_000520"
     CDS             complement(93337..94632)
                     /locus_tag="ACHKW5_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO7509967.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLANNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLINPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVTLLNTQLSEFAPTMPHNEAMKS"
     gene            complement(94652..94903)
                     /locus_tag="ACHKW5_000521"
     CDS             complement(94652..94903)
                     /locus_tag="ACHKW5_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7509968.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            complement(94975..>95170)
                     /locus_tag="ACHKW5_000522"
     CDS             complement(94975..>95170)
                     /locus_tag="ACHKW5_000522"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7509969.1"
                     /translation="PCPWRYYNDRDVRTIVELGKAIDFDVRTAIPFEGERHNALDDAR
                     YQAKYVSAIWKKLIPSQADF"
BASE COUNT        23886 a        23532 c        24569 g        23183 t
ORIGIN      
        1 taaacagagt tgtttgccac catctcaaat ctgtcgtgga taaatcctcc gtagccttta
       61 cacttaggta gcaataaagg cacacagatg cagcaaagga ataaatcatg ggatctgatg
      121 cgaaaaactt gatgagcgac gggaatgtgc aaattgttaa gaccggcgag gtcattggcg
      181 cgacgcaact tactgaaggc gagttgattg ttgaagctgg cggaagagcc gaaaataccg
      241 tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt
      301 acggtaacaa tggcacgcta tcggtcagcg acggtgccat tgccacagat attgttcagt
      361 ccgagggagg cgcaattagt ctctctacgc tcgctacggt taatggccgc catcccgaag
      421 gtgaattcag cgttgatcag ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc
      481 tgcgtgtact ggaaggacat cgcgcggaaa aaatcattct cgatcaagag ggcggcctgt
      541 tggttaatgg gacaacctca gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc
      601 caggtgggga agccagcaat tgtgagatta atcagggcgg cgtttttatg ctggccggga
      661 aagccagtga tacgttgctt gctggtggca ccatgaataa tctcggtggt gaagactctg
      721 acactattgt tgagaatgga tccatttatc gtctggggac ggatggcctt cagctctaca
      781 gttccggtaa gacgcaaaac ctgtccgtga atgtgggtgg tcgggctgaa gtgcatgccg
      841 gtacgctgga aaatgcggta atacaaggtg gaacagtgat cctgttgtca cccaccagcg
      901 cggacgaaaa ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc gggagtgttg
      961 cattactgga cggcgcttca atgattattg gctatggcgc agatctgcaa caatcaacga
     1021 ttactgtaca gcagggcggt gtgttgattc tcgacggcag tacggtaaaa ggtgacggtg
     1081 tcacttttat tgttggtaac atcaatctga atggcggaaa actgtggctg atcactggtg
     1141 cggcaacgca tgtgcaactg aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc
     1201 aaaccagtgc gaaagaaatc tcacctgact tcatcaatgt gaaaggggaa gttaccgggg
     1261 atatacacgt tgagataaca gatgccagtc ggcaaactct gtgcaacgct ctgaaattac
     1321 agccagacga agacgggatt ggcgcaacgc tccagcctgc gtaaaaacac aggcgcaacc
     1381 cgtgaaggtt gcgccttgtc tttattagcg ggatgctcgt tgcagaatga taactgacgg
     1441 cagacgttgc aggaaccgca tacgcagcat catcataatg gctgccgacg tcaggccaat
     1501 aataaagcct atccagaagc ctgctggccc cataggttca acgaccagat cggtcagtgc
     1561 cagaatatag ccgcttggca agcccagcac ccagtaagcc gtaaaggtga tatagaaaat
     1621 ggaacgcgta tctttataac cacgcaaaat cccactgcca atcacctgga ttgagtcaga
     1681 aatctgatat accgccgcca gcaacatcaa atgcgcagcc agcgttacaa cctcgggatt
     1741 gtcgttgtac aacagggcga tttgctcccg cagtgaaacc gtgaatatgg ccgtcagggt
     1801 tgccatacag acacccacca taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga
     1861 gccctgaccc agacgataac ctacgcggat agttaccgct gccgccagcg acattggaag
     1921 cacaaacatc agtgaactaa agttcagggc aatctggtgt cctgcgacat caacaatacc
     1981 gagcggagac actaacagag ccacgacggc aaacagtgtc acttcaaaga atagtgccag
     2041 cgcaatcggc aaaccgagtt gaatcagtcg tttcataacc gcaggatcgg gtttcgcggt
     2101 gcctttttcg ttacgaatat cgcgcatgga gcgggcgcgt ttaatgtaag aaaccatggc
     2161 aaggaacatg acccaataca ccgccgcagt agccacgcca caaccaacgc caccgagctc
     2221 aggcataccg aaatgaccat aaataaagat atagttcacc ggaatgttca ccagcaggcc
     2281 gataaagccc attaccatac ccggcttggt ttttgccaga ccttcacatt ggttacgggc
     2341 aacctggaag aacagatagc ccggcgcgcc ccacaacaac gcacgcagat aacccacggc
     2401 tttgtccgcc agagccggat cgatgttttc catggagcgg ataatgtaac ctgcattcca
     2461 cagcacaagc ataatgagaa cggaaacaaa acctgccagc cagaaacctt gtcgcacctg
     2521 atgcgcaatg cgctcacgtc gaccggaacc atttaattgc gcgataaccg gcgttaatgc
     2581 cagcagcagt ccgtgaccaa agaggatcgc cggaagccag atagaagtac cgatagcgac
     2641 cgccgccatg tcggtggcac tatagccgcc cgccatcacg gtatcgacaa aacccatcgc
     2701 agtttgggcg atttgcgcga gaatcaccgg gattgctaat gctaataaca gacgcgcttc
     2761 actgatatac ttctgcacgt gaacaccttt tatttgtagt tatatgagag actaaaaagc
     2821 cgcctgaatg ggcagcaaaa agaagaagca ggggaaattc agtctattgt agcgaggatt
     2881 tattaattct ccagtgaaaa aatagtcatt cttacccctt cactggtaag ggcgtttgcc
     2941 acctgctatt gtgctgaaca gaatgtctta actgatttca ggagttgtaa gtatgtttac
     3001 ggggattgta cagggcaccg caaaactggt gtcgattgac gagaaaccaa attttcgtac
     3061 gcatgtggtg gagttacccg accacatgct ggacggcctg gaaacaggtg cttccgtggc
     3121 gcataacggt tgctgcctga ccgtgacgga aattaacggc aaccatgtca gttttgacct
     3181 gatgaaagaa acgttacgca ttaccaatct tggcgattta aaagtggggg attgggtaaa
     3241 cgttgagcgt gcggcgaaat tcagtgatga aattggcgga cacttaatgt caggtcatat
     3301 tatgaccacc gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc agatctggtt
     3361 taaagtccag gatagtcagt tgatgaaata tatcctgtac aaaggattta ttggcatcga
     3421 cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc
     3481 ggaaactctg gaacgcacga ctcttgggaa gaaaaaactc ggcgcacgcg tcaacattga
     3541 aattgatcca caaactcagg cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga
     3601 aaatgccatg aatcaaccag gtacagaagc ctgatctgaa atcgcccccg gagagggggc
     3661 gatagaatag cctttagcga gcgactcgaa ggccgttttc aacaccgcgt gagaacacga
     3721 cctgccagag ttgaatatca cgggcgcgga aagcacctgc acaggcattc agataatagg
     3781 taaacattcg tttaaagcgt tcactgtagt tatccgcaat ttctggccat gcggcgagga
     3841 atcgttcata ccacgccatc aacgtagtat cgtaatcagc accgaagtta tgccagtctt
     3901 ccatcacgaa gtggggttcg ctggattgag caatctggcg tacagagggc aggcaaccgt
     3961 tcggaaaaat atatttatta atccagggat caacattcag atcggttttt ttcgaaccga
     4021 tagtatggag caggaatata ccttccggtt tcaaattacg atccaccacc gcaaaatagg
     4081 tatcgtaatt tttcggtccg acgtgctcga acatccccac agaaacaata cgatcaaact
     4141 ggtcgttcag gtcacgataa tcttgcagca aaatggtgac atccaggcct tcacagcgtt
     4201 cctgagccat tttttgctgt tcggcagaaa tggtgacgcc gaccacgctt acgtcatagt
     4261 tagatgccat gtagtgcgcc agtccgcccc agccgcagcc aatatccagt acgcgcatcc
     4321 ctggttttaa ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg gcagattcca
     4381 gattatcggc atctttccag taagcgcagg aatattgcat gaagggatca agcatgcggc
     4441 tgaacaagtc attacccaaa tcgtaatgct ccttgccgac tatccaggca cgttttttac
     4501 tctgcagatt gaagagacga gcgctggcaa tacgcagcgt gtctttgaaa tgatggggga
     4561 gttggttctc gagacctgcg cgtaagactt tgctaaaaaa catatccagt cggtcacatt
     4621 cccaccagcc atccatataa ctttcgccta accccaaaga gccttcttgc agaacgcgtt
     4681 taaaaaaatc ggggtttttc acacgaatat ccgccggggc agaaccgtta atggctatac
     4741 cggcacggct aagtaattcg ttggcgatac ggtaccagtt gtcatccggt acactgactt
     4801 cttctataca cgatgaactc atagtttctc catcactggt cggtgatcag aaccttaaaa
     4861 cagcgtagac gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa
     4921 tccgacgtag aacagaggga aaggaggaac ctttgccgaa atgaccatcc atagtgagtc
     4981 gggaacgatc ctgtccccgt aaaaacataa tatttatcgt agctttaaca actcgaaatt
     5041 agtataggcc gctaatttca atcatacaac aaaattttgt tagcagccta agtataagta
     5101 tatcgatata gatcagtgtg attcgctatg agcgacttcg gcattgccat gattctggca
     5161 gccaacttct tcacaacgtt gcatcatgta acccagagcg accagcacta ctgttgataa
     5221 catcacgctg gtggtggtga gcaatggcgt gctgatactg atcagccagg aaactaccag
     5281 acttgcgagg aagcacagac ccagttgtag agtgttctgc aacgctgcgg cgcgaccagt
     5341 tgcgtgtggg aagggacgca gcgcctgggc gacaacaatc gggtagatcg cgccattggc
     5401 aatcgccatc acacagaatg ggatcaggat ttcgaccagc gacacatggc taatgaagcc
     5461 tgcagcccag gtcgcaatga cgctgactgc aaacagcacc agcaaccacg gtaataactg
     5521 cttgccttgc catttctgca gcgcggcgcg acagccataa ccaccaatca gaaacgcgat
     5581 agtttgcggg acataactta aaccaataac tgccgggctg tagcccattt cactaaggat
     5641 gaacggtgaa ccggtcagcc atgcaaaaaa actggctgaa caggccgcgt atatcagcac
     5701 gttgccgcga taggttttag aacgtagcag gtcggtaaag gtcagaccat cctgactatt
     5761 gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca ccacggtaat
     5821 ggcaaacagg gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa
     5881 cagaggagcc agggccggag atagacccac cagcggcatg atggtcgcaa aaattcggtt
     5941 aactttctgt gaaggataat aatccgtcac taacgcttgc cagataaccg ccgcggcgca
     6001 gacacccaca gcctgtacaa aacgcaatac cagcagcgta gcggcgtttt ctacccacag
     6061 catccccaga ctacctaacg caaaaattgt caggccgatt aataacaccg gtttacgacc
     6121 ataacggtcg gagagtggcc cccacagaag ctgggctgcg gcaaaaccgg caaggaacag
     6181 actaaggctg gcactgacag cagacgcagg cgtttgcagg tcggcctgta tggcggcgaa
     6241 agcaggcaga tacatatcgg ttgccagaaa accgagtacg ctcaaacccg ccagccagac
     6301 taaaaatctt ttcccaggtt gcatgtatat tctctgaatc tctcaattaa acaccgccgc
     6361 aaagtgtagg gagtgaaatt agtgttgtga aacggtaata tttgatgact ggtttcaaaa
     6421 attttgcagg cagactatgt ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg
     6481 taatggtagt tttagcgctg cggcacagga gctgcatcgc gttccttctg cggtcagcta
     6541 taccgtgcgt cagctggaag agtggctggc ggtgccgctc tttgaacgac ggcaccgtga
     6601 tgtggagctg accgctgctg gagcgtggtt tctcaaagaa gggcgctctg ttgtcaaaaa
     6661 aatgcagatc acccgccagc aatgtcagca gatagcgaac ggctggcgcg gtcagttagc
     6721 tatcgcagtg gataatattg tcaggccaga acgtacacgg cagatgatcg ttgattttta
     6781 tcgccatttt gatgatgtcg aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga
     6841 tgcgctttcc gacgggcgcg tggaactggc gattggcgca acacgcgcga ttccggtagg
     6901 cggtcgttat gccttccggg atatggggat gctaagctgg agttgcgttg ttgccagcca
     6961 ccacccgctg gcgttgatga atggcccatt cagcgatgat acgttgcgca actggccgtc
     7021 gttggtgcgc gaagacacct cgcgaacgct acctaaacgt attacctggt tgctggataa
     7081 ccaaaaaaga gttgtcgtgc ctgactggga atcatcggca acctgtatct cggcaggatt
     7141 atgcataggg atggtgccaa cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt
     7201 agcacttgaa ttggaaaacc ccttcccgga ttcggcatgt tgtctgacat ggcagcaaaa
     7261 tgatatgtcg cctgcgttaa cctggctgct tgagtatctc ggcgatagtg agacgctgaa
     7321 taaggagtgg ctgcgggagc cggaagagac tcccgcaacg ggtgattaac gacgatagtc
     7381 gcggaacggg ccgtcagcca cggagcggcg ttcaatcaag cgcggatgca cttcaatgga
     7441 ctgcggttct tcacgtttgt tgacgatacg atccaacagc atgttgaacg ctgtttcacc
     7501 cagcgaatct tttggctggt ggatcgtggt cagcgccggc gtaaaatagc gggcgttgcg
     7561 cacgttatca taaccgatca gcgaaacatc ctgcgggacg cgcagaccca tttcatcagc
     7621 agcacaaagt gcgcccattg ccatgatatc gccaccacag aagacggcag tagggcgatg
     7681 cggctgcgac agtatttgct gcatggcgcg ataaccagat tcgggttcaa agtcaccctg
     7741 aacaatccag ctttccggca ccttgatcat cgcttcttcc atcgccttca taaaaccggc
     7801 aaggcggcct gcgccggtgt tacgttccag cgggccgggg atgacgccga tttcgcggtg
     7861 accgcgttca atcagataac gcccggccat gtagccgcct tcgaacgcgt tatcaatgac
     7921 cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc accaccattg ggatatggcg
     7981 atactcttcc agcatcgcca gcaacggctc tgggtactca gaacacatca ccagcagacc
     8041 atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa gattgttcca
     8101 cgcattaccc agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa ccgcttcaat
     8161 gatctcggca aaataggccg cttcgctgct ggtcgccagc aaaccgatag acttggtgtg
     8221 gttaaccttc aggctacgcg ccaccgcgct aggggagtag tgtaattctt taatcgctgc
     8281 ccacacggcg ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatta cgtgtgacac
     8341 agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt attgttgcca ttccatttca
     8401 ctccagaccc taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc
     8461 ttacgcacaa caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt caggaagggg
     8521 tcaatggcca gtacgcaaat agtgtaagcg tggaattttg gccgatcttg acgaaaaggg
     8581 gaagtggtaa aaacacttat cattataaat gacgggagat tttttcatca cagtgtgtaa
     8641 aaatgcgtaa tatcactatt tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga
     8701 ccttaaattt tcactggtaa caacgattat cgtcctcggt ttgatcgtgg ccgtgggttt
     8761 gactgccgcg ctgcactgat ttcctctcga gggagcgcgt tctccctcgc atctccttcc
     8821 tgtttgttag caaaatggca aaagtgtttt ctctttttgt tattttcatt tttttgtgat
     8881 tgctgtcatg ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga ggtggagata
     8941 aggcatgaaa attaactatc cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac
     9001 aacggagttc tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat
     9061 tcccgctgcc ggaatgttaa tcagtgccta tgcagttggc gtaatggttg gcgcgccgct
     9121 gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat
     9181 tttcacgctc ggcaacgtac tttccgccat cgcgccggat tatatgaccc tgatgctttc
     9241 acgcattttg accagcctga atcacggagc attttttggt ttgggttcag tcgtggccgc
     9301 aagcgtggtg ccaaaacata aacaggccag cgcagttgcc actatgttta tggggttaac
     9361 cctggcaaat atcggtggcg tgccggcggc gacctggttg ggtgaaacca tcggctggcg
     9421 gatgtcattt ctggcaacgg cggggctggg agtgatttca atggtaagtc tgttcttctc
     9481 attacctaaa ggtggtgcag gggcacgacc tgaagtgaaa aaagagctgg cggtattaat
     9541 gcgtccgcag gtgctgtctg cattgctgac gacggtactg ggagctggtg caatgtttac
     9601 tctctacacc tatatctctc cggtactaca aagtattacc cacgcaacac cagtgttcgt
     9661 cacggcaatg ctggtgctga ttggtgtcgg attctctatc ggtaactatc tcggcggcaa
     9721 actggcagat cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat
     9781 tatgctggca atcccgttcc tggcccgcaa tgagttcggc gcagctatta gcatggtggt
     9841 gtggggcgca gcaacctttg cggtcgtacc gccgttacag atgcgcgtga tgcgtgtcgc
     9901 cagtgaagcg ccgggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc
     9961 gctgggagca gctgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc
    10021 ggtgatggga gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa
    10081 acaacctgaa acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc
    10141 ttctgcgtta cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct
    10201 catccgcgtt ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc catcagttat
    10261 tatgcagcga gatttttcgc tacgaactcc cagttcacca gcgcccagaa gtgctccaga
    10321 tagccaggac gtgcattgcg atagtcgatg taataagcgt gttcccagac atcaacggtc
    10381 agcagcggag tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc
    10441 agtttgccat cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct
    10501 gcatcagtaa actgcgcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca
    10561 gcgacttttc cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta
    10621 tggttccaga cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct
    10681 tccagtgatt taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa
    10741 gtctgatgat gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga
    10801 gcatctttag catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat
    10861 taatttgcat acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt
    10921 gagacaaaag atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt
    10981 ttttggggtc attacccgac gagcgccaac atagtggcgc tgccagtagt cttcactgag
    11041 agaagtgatt tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac
    11101 atacacgccg acatgatcgg ctgtgccgcg tccctgagta cggaaaaaga ccaggtcgcc
    11161 gtttttcagt tcactacgtt cgattggcgc tgcatcacgc aggtgataca tttcattcgc
    11221 cgtacgcgga atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca
    11281 atcaaaaccg gtacgcggtg agctgccacc ccaacgatat ggcttaccaa tttgctgcat
    11341 cagtttattc attgccactt tcgtagcttt ttgcaccttc gctttgtgcg cgtcggcaag
    11401 agttcctgac gcggcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt
    11461 acacttttcg gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt
    11521 ggcgttttta gaagcggtaa tggaagattt tttaacggaa gaagcggttt tgcttgtggt
    11581 cgtactttta ctggcggctt ttttcgctgt tttctgggtc tttttggtgg ttgccgtgct
    11641 tttcttttta tctgcttttt tggtgatatg agtggtagca gaactctccc tggcttgctt
    11701 tgaagcatgg gccataggcg ttaaaggcag ggtggtaaaa agtaaagcac agagcgtgat
    11761 cgaaatacgg tttatccgcg ccactgagcg tccctctgtt aaggcaggtc tatcatgcct
    11821 gcgtctgatt tacaaaactt gatgattcta atgcatagaa gaagagaaag ttaatagctt
    11881 ttattgttaa aaaatacgtt tcgttggcga gcgcaaaaaa atgcagcagt gaaagctaaa
    11941 aatggttata aatacgccag tttgccgcgc atcagagaag aaacgaatat ttgaaatgca
    12001 acaaatagag caaagggata aaatagcaaa agcgtgatga aaatgttatt ttccgatgcc
    12061 gcactgaccc gctacaatgt cagattactg ccgtaaagaa gtttgaggaa gcaagacaat
    12121 gagcaccact atcgaaaaaa tccaacgcca gattgctgaa aacccgatcc tgctgtacat
    12181 gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc caggcagtcc aggcgcttgc
    12241 cgcatgtggc gaacgttttg cctatgttga tattctgcag aatccggaca ttcgtgcgga
    12301 actgccgaaa tatgctaact ggccgacctt cccgcaactg tgggttgacg gcgagctggt
    12361 cggcggttgt gatatcgtga tcgaaatgta tcagcgtggc gaactgcagc agctgatcaa
    12421 agaaactgcc gctaaataca agtctgaaga gccggacgcg gaataatccg tcctgcaata
    12481 ctgacggatg cgaacgggca tccgtcagtc ctttctctat ccccaatcca gcccttgtgg
    12541 cgaacttgaa aatcccgcct cacgcagcag cgtaaacatc ggcgtttgcc ggaccggtag
    12601 atcattcact tctgttagcg taaagcgcag ccgtggttcg cgacgcagtg cggtagtcag
    12661 cgcgtggaaa acctctgggg cgagtaattc ctctttttcc tgccagacca gcattttttt
    12721 gccaccttgc gccagataga gtaacaattt gccgccagaa acgaccacca gcgcacccgc
    12781 ccgacgcgtt ggaaccagcg ttgccggatg tgcaggccag ggcagaagat ttccccacac
    12841 attagccgga tcgttggcag agagcgccac tggtgtatag tggcgcgttt gcgtggcttg
    12901 tgtcgccaga tcgcgcaatc ggtcaatagt cagacgttca gcgaattgcg cgccacccag
    12961 accttctaca aaacgacctc gcataattcg cccggagtct tccatacttc gacaaagcgt
    13021 ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac gactgatgat
    13081 gccgtagcgg tcgagcatat tttccgccag cgccagcatc ctttcggtat cgtttagtgg
    13141 ctccacctgc aataacgacc agcgtccagc cagatttggt gtgttgtaag ataccagcgg
    13201 cgagacgggt tgcgcataga caggacgtcc acgacgagcc tggtgactgc ggcgagttga
    13261 ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa tgtcgctggt
    13321 gatgacgcct tgccagacca gcgcccataa cgcctcttgc agggcagaga gatcaaccga
    13381 ttcgccgatt ttgtcgcgta tccgttggct gatttgttgt gcaaaccagg ctcctccgtc
    13441 agccagaaca gcgactatcg cttgttgcag cgccgaacga ttcgcctgat ccgcttcggc
    13501 gggagtgaac gattctgcag catattcctg tagatgcagt gccaccaggc cgtcatcttc
    13561 acccagtttt ttttgccccg accagataac cgcaccggtt gccagtaatt catcgagcat
    13621 ttctgacgaa tagtcgcgta cgcgggcagg caggatctgg ctttcccaga gtgaggcggg
    13681 taaaccgact ccggcaagct gttcgatcac ccgcatcacg ccatctacgc cctcataaac
    13741 gcctggcgat gttgaggcaa agagcgccgg gctaccatcg gtggcgggta atacgccctg
    13801 acgttccagc agcaatcgcg cataggtcgt ggctgcaacg ggacgcgtcg cttctctggc
    13861 ggcttgcagc gagcgcaaac gcagacgacg aaatacttca tcgctcaccc agatgtcttg
    13921 ttgcagattc atcaccagac cctgttcacg cagttgctga agctgctctt cgacaatggc
    13981 aataccgaga ctaaactcat gagccagttg ttcagccgtg accagagcat gagcccggag
    14041 atagcgcaga aagagatcgc gaagcgggta actgactcta tgtaaataaa tctctggcaa
    14101 tgactctggt agtcgtacgc cgagggcatc acgcagcctg gcggcatcat ccatacaggc
    14161 aagacgctcc tgtccgctaa tcatcgctgg gaagattcgt tttactgcaa gaagattttc
    14221 cagatacgac gcaacctctt cactgctgcc tgtatgccgt tgcgccaggt cttcaacggt
    14281 cattggcccc agttcgcgca gcaggtcgaa caatccttct tcacctttcg ctcttctgcc
    14341 aggagccagt cgttgcaact cttcttccac ctggcgaatg acctgcgggt cgagtaattc
    14401 ccccggatcg acctgtccga gtagattgcg cagtaactcg ctgtccagcg acagtacgga
    14461 tgcccggcgc tctgccagcg gggcgtcgct ctggtacata aattccgcga catagccgaa
    14521 caataaactt gtggcgaaag gcgagggcgt agtggtcgtt acatcggata tttgaatttc
    14581 gccaccgttc aggcgacgca tcaaacgttc cagtgcggga agatcataaa catcttgcag
    14641 acattcgcgt agggtttcga gaatgaccgg aaaatccgga tatccctgag cgatttccag
    14701 caactgactg gcgcgcagcc gttgttgcca aagcggggtg cgatggcccg gagtgcgccc
    14761 cggcattaat aatgcccgcg cggcgcattc gcgaaaacgg gcggcgaaaa gtgccgagct
    14821 gcctaccgcc tcgcggacaa tttgcagcaa cttttctggt tcaaacaaaa aaatcgcggc
    14881 gtcgggcaat ttaccatcag tgtcaggaat gcgcgcaaca atgccgtcat cactggcgac
    14941 cacagacgcg tcagcgcccc atagggcatg tattcgcccg gcaatcgcca gcgcccaggg
    15001 ttcatgcacc cgtcttccat agggagagtg caaaataata cgccagtcgc caatttcatc
    15061 acggcaccgt tcgaggacca gatggcgact ccccggaacg atgcccgtcg cgttgcgttg
    15121 ctcgtcaatc aacccctgaa tattggcgat cgtattttct tctgccagcc acgggggaat
    15181 agtcccggaa aagaacgcgc catccgccag caaatgaaga aaatcgccga tcatctcgcc
    15241 taattcagcc ggacgtccgt taccttcacc acgccagaag gggagccggg cagaacgacc
    15301 cggagcagga gtcacaatca cctgatcgcg ggtgatttgc tggatccgcc atgaggtagc
    15361 gccgagagtg ataatgtcgt tcacccgcga ctcatatacc atctcctcat ccagttcacc
    15421 cacccgccgc gaaccggcct gttcttcacc ttcgggtaat aacacgctat acatgccacg
    15481 atccggaatg gtgccgccgc tggtaaccgc caacaattga gcgccaggtc gggcggtcaa
    15541 tatcccggtc tccctgttcc agaccagttt ggggcgaaaa gcagaaaaat cgccagaggg
    15601 atagcgcccg gaaagcatat ccagcgtggc gtcaaaaaca cgtcttggca gatctttcca
    15661 cggtgcggca cggcgtacgc gggagtacca ttcgtctacc tgtaatgcct ccatcgccgc
    15721 ggcggcaacg gtttgctgcg caaggacgtc gagtggatta tgcggcggtg tcaggttttc
    15781 cagcctgcct gcgaacatac actctacaat gactgcggaa tcgactaagt cacgccgggt
    15841 acgagggaaa aacagccctt tagatacacc gccaacctga tgtccggcgc gaccaatgcg
    15901 ttgtaaccca ctggcaacag aaagcggcgt tgccacctga atcaccagat ccactgcgcc
    15961 catatcaatc cccagttcga gactggaggt tgcgaccacg cagcgtaact cacccgattt
    16021 cagcgcctgc tcggtgattg ctcgttgttc tttagagacg gagccgtggt gtgagcgggc
    16081 aataaaaacg tcgctacttt gtacacggtt agaggttgcg ccggaggtcg actcgaaatg
    16141 ggccgcatca acggcgatag acggggaacg ctgtaatcgt gcggcgtaaa gctcatttaa
    16201 tcgtgccgtc agtttttccg ccagcccacg tgaattagta aagacaatgg tcgagcgatg
    16261 gcgcaacact tcatcaagga tacccgtttc aatatatggc cagatggagc cttcccggcc
    16321 ggcatggctg tcttcgccgg tgctgctggc gaccgatgag acatcatcca tattggcgac
    16381 cggtacgaca attcgtatct gcggatggcg cattgcgggc gggttgacta ccgtaaccgg
    16441 gcgatcgcca ccaagaaatg ctgccacatc gctggctgag cgcacagtgg cagaaaggcc
    16501 aattcgctgt gctgaggtgt ggagcagcgc atcgagccgc tccagactta acgccagatg
    16561 cgcaccacgt ttactgcccg ctaccgcgtg gacttcatca ataattaccg tttcgacgcc
    16621 gcgtagcgtt tcgcgcgcgc gggaggtcag catcagatag agtgattcgg gtgtggtgat
    16681 cagaatatcc ggcggattac gggtgagttt gctgcgttcc tgtgcaggcg tatcgccagt
    16741 acggatccct acgcgaagat tgacttccgt ttcgccgcgc cgccgccgtt catcggcaat
    16801 acccttcaac gggagctgca agttgcgctg aacgtcggtg cccagagctt ttatcggtga
    16861 aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt cgccgccttc
    16921 gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg agccggtcgg
    16981 tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc caggtttgcg actggacagc
    17041 ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa acacgtccgg
    17101 caggagcgat gaagagtctg gattatctgc cattaacgct attccccgtg aatatcatgc
    17161 aggatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat caggcatcga
    17221 ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt ccagcggttg
    17281 acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga gtgcgcctgg
    17341 gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt ttgtccgagt
    17401 gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg gttacgtttc
    17461 agtgaggcgc gttctgcggc ggccatcata aagctgtgat caaaattggc attgtgcgcc
    17521 accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac tttaaaaatt
    17581 tcgtgcagcg cctcgtattc gctgaccgcg ccgcgatcgg gatcgttcgg gtcaatgccg
    17641 ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa atggttcgac gtggaaatgt
    17701 aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat ggcggcaatc
    17761 tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat cacaacagga
    17821 taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga catctgattc
    17881 tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg atgcagtaaa
    17941 gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc gcgaccggcg
    18001 tctttctctt cgattaactc aattttgtaa ccgtccggat cttccacaaa cgcgataacc
    18061 gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt ttgacggatt
    18121 ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata agcagtgccg
    18181 agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc ttcggtttcc
    18241 gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct ggtacgcagc
    18301 agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcagatc gccaacgcgc
    18361 agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga ttcaaaatgt
    18421 tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac gtcgggtaat
    18481 cggtatagcc ttccgcgccg ccaccgtaga aactttcggc acgctgtggg ttaagctcag
    18541 ctttgcgctg caagcgggcg accagatccg ggttcgcaat ccagtcacga ccaaatgcca
    18601 ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg tatgcacctg
    18661 cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca tcagtatacg
    18721 gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg cgtttaccca
    18781 gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatcc gtgttctgga
    18841 aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc accccattct tcaatcccgg
    18901 catcgaccac ttccagtacc agacgtgcgc gattttccac gctgccgccg tactgatcgg
    18961 tacgatggtt tgaagaagga gaaaggaact gatgcagcaa ataaccgtga gcagagtgga
    19021 gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg aaatcattga
    19081 cgatacctgg aatctcttcc agttcaagcg cacgcggcat ggatgtttca acacggatcg
    19141 cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct gaaggcgcta
    19201 ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg tgccacagct
    19261 gcacggccat atgaccattt tcagcatgaa cgccagcggt gatttttttc catgcggcaa
    19321 tttgctccgg actatggatg ccaggcgcac ctgcatatcc ttttgcctgg gcagaaattt
    19381 gcgtggcttc actaataatc aaaccggcac tggcacgttg gcgatagtat tccgccatca
    19441 acggggtagg aatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt gccataaaaa
    19501 tacggtttgc cgccgtgatc gcgcccactt tcagtgggga atacagtttt tcagatgaca
    19561 taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc gtcgcaataa
    19621 tgttttttac atgggccagc gcgttttcca gtggctcgaa actgcgcgaa attttggcct
    19681 gcagattcgc acccagccat agtgcgtaaa gcacttgtgc ctgttgtagc ggttcgccac
    19741 aaaaggttaa acaatggttc tcacggccat tttccagcgc ctgagagagc agggcgatca
    19801 cgccgcgagc acctttatcc atcgcgctgc gcatatcttc tgacagatcg cacacttcgg
    19861 cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac tggttcagtg
    19921 tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat tgcagcaact
    19981 cagtcagtcg ctggtgatat gcggcgtaat gacgctcaag catggcaacg ccaaacgctt
    20041 ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt tttagtaatt
    20101 cgcttagccc catcccggtg aatccacgtt gcaggcaaag ttgctcgccc gtcgccagga
    20161 gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta gtagaccagt
    20221 cggtctagtg caagcaaagt tgctactttg gacattaaca gcttcaataa tggtaataat
    20281 cttatctgag gagggcgaag tggcggagca attagagttc tttcccgtcc agagcccgtg
    20341 ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc gtagtcgtga
    20401 tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtgc tgcgcctttg
    20461 tcggcagcga ttaatgcgca aattacgcgc aaataagcca gcgtcatcag acgaaccgga
    20521 gcaaccatca ctcttttgaa cctaatcttg cgtatactcc tctcatatct gataagagga
    20581 agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt cccgttttgt
    20641 gatgggctac tggcgattga tggactggaa tatgtccgcc cgccagctgg tcagttttat
    20701 tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt atggtggcta
    20761 tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca cctcacctgc gtgaacggat
    20821 ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg tcattggtca
    20881 ttacatcact gaccgcgatc acatcattaa gagcgccgaa cagtcgctaa ttaatctcgc
    20941 gaccgatcat ctggatctgc tgttaatcca ccgaccagac ccgttaatgg atgccgatga
    21001 agtggcggac gcgttcaaac atctgcatca gagcggcaaa gtgcgtcatt ttggcgtatc
    21061 gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt ctgccgttta cccttgccac
    21121 taatcaggtg gaaatatccc cggtgcatca gccgttactg ctggatggca cgctcgacca
    21181 actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg gtcgtctgtt
    21241 taatgatgat tatttccagc cgctgcgtga tgaactggct gtggtggcag aggagttaaa
    21301 cgcgggctcg attgaacagg tggtttacgc ctgggtatta cgtttaccat cgcagccgct
    21361 gccaattatc ggttcaggta aaattgagcg cgtacgggca gctgtcgaag cagaaacact
    21421 gaaaatgacc cgtcaacaat ggtttcgtat ccgtaaagcg gcactggggt acgacgtacc
    21481 gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc actggtttac acttattcag
    21541 gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc tggctattct ggcgctggtt
    21601 gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt cacgtcgcaa
    21661 ggggtagggc agtcaattgg tagcgtcacc attactgaaa ccgataaagg tctggagttt
    21721 tcgcccgatc tgaaagcatt accccccggt gaacatggct tccatattca tgccaaagga
    21781 agctgccagc cagccaccaa agatggcaaa gccagcgccg cggaatccgc aggcgggcat
    21841 cttgatccac aaaataccgg taaacatgaa gggccagaag gtgccgggca tttaggcgat
    21901 ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg atgccgtcat cgcgcctcgt
    21961 ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg tccacgttgg cggcgataat
    22021 atgtccgatc aacctaaacc gctgggcggt ggcggtgaac gctatgcctg tggtgtaatt
    22081 aagtaattac gaggccagcg taccttgcgg tggtgcttgc tcaagttgcg aaagcgagca
    22141 gtacagccgc cagacaattg ccgccagctc acgggcggca ggttgatgat gacgggcaag
    22201 gctgttgcaa atccgctgta attcttcaag tgtggccgcc agtgattttt gctgaacgcc
    22261 acgctcactc atcactcccc gcaacagtga aatacagtta tcccgtactc gcgataacgg
    22321 atcggaacgc gattcccagt cgcgcaattg ccagacaaca tgagaacagt tcagcagcac
    22381 tacaccccag cgtaataacc aacggcgagc cagcgcatcc tggctgttac tcaactgact
    22441 gacgtgatga taagtgagcg attcaaattc gctttcactc agtgttggat ggcggcttag
    22501 ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg cggcttttac gagcatccga
    22561 tcctggacgc tgaatggcga acgctagcca agccagcgca acgccaacga tttttgccag
    22621 attatcgtta agaaaatcag caaaatcata caccggggga ttagtgacag cgataaacga
    22681 acccataaaa acaatcagtt gcccccacag tgcggcaaat ttgggcatct gcgatttaag
    22741 aagctgcatt gtcgccagca gtggaaagag aaacagtaaa aattgccaca gatcgctaat
    22801 ctggaccatc aggccaaatt tgaccacaaa gctgaatagc gaaagtaaca ccagcgtgcg
    22861 catcagcagt gacaacgact taaacggtgc tgcgacggcg gagtagagta cgcagctaat
    22921 tgctgccagc gttaatgcat tggcaccgac atcccattgc gatgcaatac tccatgcgcc
    22981 aatcatcatc aacgtacaaa atgtacgcag cccgctccac atagcttcgg cgttgtcggt
    23041 atgacgagcc agagcggatg tccgtgcgag tctggcatga tcatctacac cgctttgcag
    23101 acgatgtaat tcctgactac tttgcagata aaggcggcaa aaataacgta gtcgctgcca
    23161 gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt agcggggcga taatacgtgc
    23221 gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc agcaattgtt cgagaatttc
    23281 tcgcgtggca cctggcggtg agggccagtt gagcaacata cgtcgcaggc tggagatgac
    23341 actggtcata cgtaattgct ggtggagtag cgcattaagg cgcgcgtttt gctggcgaaa
    23401 acgatagtgg ctccagaaag cctggatacg cagcaaattc atggtcagta tctgcccaat
    23461 caccccctca tgtgctgcac gaatggcatc gtttgtttca ggctgccaga gtaaactggc
    23521 atgttccagt aatcgggcgt gcatgttttt caatgcggtt aaaagggctg tagcatcgga
    23581 actgctaggc aggatcatca tcatcatgcc gccgcacaaa ataccgacta tcacctcgca
    23641 aacgcgcgcc tgagcgatat cccacagctg gctggcctca gtaatattaa ccatcggaaa
    23701 ggcgatgatg gcagccgtgt agcccgccag ttgaaatgca tacgcgacgt tattcgtgaa
    23761 gtgcgcacag gcccaggtac aaaagccaag ccacgccgac atgctcaata gaaaaaacca
    23821 cggctcattg agcgtatgcc cggcaagaag cagtgccgca atggctccca gtaaactgcc
    23881 agcgatgcgt ccgaggcttt tgctgatgac accgccaacg gtgggaaagc taaccactgc
    23941 agccgaggtc atcgcccaat agggttcatc cagattcaaa taataggcaa ccgtcagcgc
    24001 cagacacatg gcaatggtat tgcgtaacgc ataacgccat tgcgccagcg tggccttgaa
    24061 ccagggcaaa ttgcgcaagg accatgacga tgcgttcatt attgacctac ggcaatgcta
    24121 caggtggtgc ccgacaccag cgtgacatcg cctggaactt tatccagcgc aaagcggacg
    24181 ggaacgcgtt gggcgagacg aacccagggg acgttaggtt tcacatccgg aataaggctt
    24241 gagtcacttt caacgctttg atcataaatc gcccgaccga tactggaaac gtgaccccgt
    24301 aacgttttgt tatcactata gagggtaatt tgtgctggtg caccttcgcg gatatggcgc
    24361 aacttggttt cttcaaaata accaatgacg taaaacgagt ggctgtcgac aagggcaaac
    24421 agcggtttcc cggtgtcagc gtaatcgccg atgcgggtag tgaggttagt cacccaacca
    24481 ctcaccgggg cgcgaatttc cgtttgcgcc agttgccatt gtgtctgttt cagcgtggcc
    24541 tgtgcggcat taacactggc ctgcatcgct ttaacattga ggttagcggt atcgagttct
    24601 tcggcagaaa taaaattttg tgagagatga cgacgacgat tcgcttcgtt attggctttt
    24661 gcgaggtcag attgcgcttt cgccagctga gcctgtgcgt tcagctcggc gatctgaaag
    24721 ggcgttttat cgatcgtgag cagaagatcc cccgcgttaa ccagttggtt gtcttttata
    24781 ttcagctcaa caatacgtcc cgacacctgt ggggtgatag aaaccttttc tgcgcgtatt
    24841 ttcccgtcgc gtgtccacgg agattgcatg taataattcc acagccacca tccggcaaga
    24901 accgcaacta tcgctacaat gattgttgag aaatacttaa ttgttttaat tgacatagtt
    24961 accacgcaat cagtattgcc agagcaaggc aaacgcaaat cgcaaacagc gataaatcca
    25021 ttaacaaggg atgccagatg tcaccggcgt agatccagcc acgaagcagg cggtgtacga
    25081 caagccagat gacgaatcca aacgcgaagg ctttgaaaaa cggaggaaaa tagacggacg
    25141 caccgaacac cagatcttgt aagggcaatc ctgttgcatt gagcataaac ttcacaaagt
    25201 agattccttt acgaccgaat atattgcgtt agattaataa atattcttta agtgcgaaaa
    25261 atttacgcgc aatttctgaa aaatatgtct gacggtaacc aaatgcagca atacatttgt
    25321 tttagcaata caattgctgc acactattct aaaagccgca taatatctta gcaagctaat
    25381 tataaggaga tgaaattgga atcgccacta ggttctgatc tggcacggtt ggtgcgcata
    25441 tggcgtgctc tgatagacca tcgcctgaaa ccgctggagt taacacaaac ccattgggtt
    25501 acgttacaca atatccatca gttacctcca gaccagtcgc aaattcaact ggcaaaagcg
    25561 attggcatcg agcagccatc actggtccgt actctggacc aactggaaga aaaagggtta
    25621 atttcgcgtc aaacttgtgc cagcgatcgt cgggctaaac gtattaaact gacggaaaag
    25681 gcagagccac tgatcagcga aatggaagct gttattaaca aaacccgcgc ggaaatatta
    25741 catggcatct ccgcagagga actggagcaa ctgattacgc tcatcgcaaa acttgagcat
    25801 aatatcattg agttacaggc caaagggtga aatgaagggg gcgtgtggtc agtaacctga
    25861 ccacacgcaa acttatttag cgcggagaaa cggtcacctg actgccattg ctggccagta
    25921 cgacacgttg gcccggagag aaacgagtgt tgccttgttt ctgtaccacc atgatggtat
    25981 taccatcgtc tttacgaatt tccagctcga caccctgcgt tttgttcatt gcactctgta
    26041 cgccctgacc agctacgcca cctgcaacag cgcctgctgc agtagccaga gaacgcccgg
    26101 ttccgccacc aacagtattc cccaggaaac caccaagaac agcaccgcca attgcaccga
    26161 taacgttgga atcatcaccg ccctgaatct gtaccggacg tacgttaacg atggtgccat
    26221 agctgacatt ctgtacttgt ttcgcttcag aagcggtata aacatcccct gacagggtgt
    26281 cgttattaac acaaccgaca agagacagac ctaccattga aacaaccaat acgcgtttaa
    26341 tcattgaaaa atctcctgtt caccatgaaa cgccacgcaa gcatccctca tggtcaaagt
    26401 atatggcata tgtgaatcca tggttacatg ttctaccaaa tatgagcaaa tcataatgaa
    26461 agtaggatca accaattata aacaaaacat tcatgatgat tattgatttg aagataatgt
    26521 tttactacca acggtaaaaa tcggtcattc cgcatggtat tcacgaaaag tttatgtttg
    26581 agtggtgcgc aaacgtcaga ttgaaaaagg atgaactatg aaatcgggcc gctttattgg
    26641 cgttatgtca ggcaccagcc ttgatggtgt tgatgttgtg ttggcgacaa tagatgaaca
    26701 ccgggtcgca cagctggcaa gcttgagctg gccgatcccg gtgtctctga aacaggctgt
    26761 actggatatt tgccagggcc agcagcttac actttcgcag tttggacagc ttgatactca
    26821 actcgggcga ctttttgctg atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc
    26881 gagagatata gttgcgatcg gctgtcacgg tcaaaccgtc tggcatgaac cgacgggcgt
    26941 ggcaccacac actttacaga ttggcgataa caatcaaatt gtggcacgca ccggaattac
    27001 ggttgtcggt gattttcgcc gtcgcgatat tgccttggga ggacaaggcg caccgctggt
    27061 acctgcgttc catcatgcct tgctggctca ctcaaccgag cgacgaatgg tgctcaatat
    27121 tggcggcatc gccaatctgt cactgctcat tcctgggcag ccggttggcg gctacgatac
    27181 cggtcctggt aacatgctga tggatgcctg gatctggcgt caggccggta aaccttacga
    27241 taaagatgcc gagtgggcac gggcgggtaa agttattctc ccactgctgc aaaatatgct
    27301 cagcgaccca tatttctcgc aacctgcacc gaaaagcacc ggacgcgaat actttaacta
    27361 cggctggctg gagcgccatt tacgccattt tccgggtgtt gatccccgtg atgttcaggc
    27421 cacactggca gaactcaccg ccgtgaccat ttctgaacaa gttttgttga gcggtggctg
    27481 cgaacgattg atggtatgtg gtggaggtag tcgtaatccg ctactcatgg cgcgtctggc
    27541 ggcattactg ccaggcacag aagtcaccac caccgatgcc gttggcatta gtggcgatga
    27601 catggaagca ttggctttcg cctggcttgc ctggcggacg ctggcgggat taccaggaaa
    27661 tctgccttcc gtcactggcg caagccagga gacggtactg ggggctattt tccctgctaa
    27721 cccgtgatat cagagttaac tgaatttttc ttaccgcgtc tgccgttaca cttggaacgt
    27781 tagggagggc gtattgccct ccagaccagg aaagtcttcg ggaaatgtct atgaaaaaac
    27841 tgttactgat ctgtttgccg gtgcttctca ccggctgtag cgcgttcaat caactggttg
    27901 agcacatgca aaccgctacg ctggaatacc agtgtgatga aaaaccgttg acggtcaaac
    27961 tgaataatcc gcgccaggaa gtcagttttg tttacgataa tcaactactg catctcaaac
    28021 agggcatttc agcgtctggt gcacgttaca ctgacggaat ctatgttttc tggtcgaaag
    28081 gcgatgaagc gactgtctat aaacgcgacc gcatcgtatt gaataactgt cagttacaaa
    28141 atccacagcg ttgagatttt tccaggggcg gcgcacaata gcgtcaccca ctgacaatcc
    28201 gtaaagaaaa ccatgtctga taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa
    28261 tacaccaaag gcgggttacg ccgtcgcgat cttcccgccg atccattaac cctttttgaa
    28321 cgttggctct ctcaggcgtg tgaagccaaa ctggcagacc ctaccgcgat ggtggtcgct
    28381 accgtggatg aacatgctca gccttatcaa cgcatcgttt tactcaaaca ttacgacgaa
    28441 aaaggcatgg tgttttacac caacctcggt agccgtaaag cacatcaact tgaaaataat
    28501 ccgcgcgtta gcctgctgtt cccgtggcat acccttgaac gccaggtgat ggtgatcggt
    28561 aaagcagaac gactttcgac tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat
    28621 agccagattg gtgcatgggt ttcgaagcag tccagtcgca tttctgcccg cggtatcctt
    28681 gaaagtaaat tcctggagct gaagcagaag tttcaacagg gcgaagtgcc attgccgagc
    28741 ttttggggcg gttttcgcgt cagccttgaa cagattgagt tctggcaggg cggtgagcat
    28801 cgcctgcatg accgcttttt gtaccagcga gaaaatgatg cgtggaagat tgatcgtctt
    28861 gcaccctgaa aagatgcaaa aatcttgctt taatcgctgg tactcctgat tctggcactt
    28921 tattctatgt ctctttcgca tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta
    28981 tatacatgga gattttgatg gcaagcagta acttgattaa acaattgcaa gagcgggggc
    29041 tggtagccca ggtgacggac gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg
    29101 cgctctattg cggcttcgat cctaccgctg acagcttgca tttggggcat cttgttccat
    29161 tgttatgcct gaaacgcttc cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg
    29221 cgacgggtct gattggcgac ccgagcttca aagctgccga gcgtaagctg aacaccgaag
    29281 aaactgttca ggagtgggtg gacaaaatcc gtaagcaggt tgccccgttc ctcgatttcg
    29341 actgtggaga aaactctgct atcgcggcga acaactatga ctggttcggc aatatgaatg
    29401 tgctgacctt cctgcgcgat attggcaaac acttctccgt taaccagatg atcaacaaag
    29461 aagcggttaa gcagcgtctc aaccgtgaag atcaggggat ttcgttcact gagttttcct
    29521 acaacctgtt gcagggttat gacttcgcct gtctgaacaa acagtacggt gtggtgctgc
    29581 aaattggtgg ttctgaccag tggggtaaca tcacttctgg tatcgacctg acccgtcgtc
    29641 tgcatcagaa tcaggtgttt ggcctgaccg ttccgctgat cactaaagca gatggcacca
    29701 aatttggtaa aactgaaggc ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca
    29761 aattctacca gttctggatc aacactgcgg atgccgacgt ttaccgcttc ctgaagttct
    29821 tcacctttat gagcattgaa gagatcaacg ccctggaaga agaagataaa aacagcggta
    29881 aagcaccgcg cgcccagtat gtactggcgg agcaggtgac tcgtctggtt cacggtgaag
    29941 aaggtttaca ggcggcaaaa cgtattaccg aatgcctgtt cagcggttct ttgagtgcgc
    30001 tgagtgaagc ggacttcgaa cagctggcgc aggacggcgt accgatggtt gagatggaaa
    30061 agggcgcaga cctgatgcag gcgctggtcg attctgaact gcaaccttcc cgtggtcagg
    30121 cgcgtaaaac tatcgcctcc aatgccatca ccattaacgg tgaaaaacag tccgatcctg
    30181 aatacttctt taaagaagaa gatcgtctgt ttggtcgttt taccttactg cgtcgcggca
    30241 aaaagaatta ctgtctgatt tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa
    30301 atcactcccc ctggttttta tacagggaac atgatgaaaa atattctcgc tatccagtct
    30361 cacgttgttt atggtcatgc gggtaacagt gcggcagagt ttcctatgcg ccgcctgggc
    30421 gcgaacgtct ggccgctgaa caccgttcaa ttttctaatc acacccaata cggcaaatgg
    30481 actggctgcg tgatgccgcc cagccattta accgaaattg tgcaaggcat tgccgccatt
    30541 gataaattac acacctgtga tgccgtgtta agtggctatc tgggatcggc ggagcagggt
    30601 gaacatatcc tcggtatcgt ccgtcaggtg aaagccgcga atccgcaggc gaaatatttt
    30661 tgcgatccgg taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg tgtcgcagag
    30721 tttcatgtgc ggcacggttt gcctgccagc gatatcattg cgccgaatct ggttgagctg
    30781 gaaatactct gtgagcatgc ggtaaataac gtcgaagaag cggttctggc agcgcgcgaa
    30841 ctcattgcgc aagggccaca aattgtgttg gttaaacacc tggcgcgagc tggctacagc
    30901 cgtgaccgtt ttgaaatgct gctggtcacc gccgatgaag cctggcatat cagccgtccg
    30961 ctggtggatt ttggtatgcg ccagccggta ggtgttggtg atgtgacgag cggtttactg
    31021 ctggtgaaac tgcttcaggg ggcaacgctg caggaggcgc tggaacatgt gaccgctgca
    31081 gtctacgaaa tcatggtgac caccaaagca atgcaggaat atgagctgca agtggtggct
    31141 gctcaggatc gtattgccaa accagaacat tacttcagcg caacaaagct ctgaaatatc
    31201 tcatttcggc ccacaacgac ctgtgggccg atggatatta ctttaagcct tccgctgaca
    31261 gcgcgtcttg tacttccgga cgttcagcca tacgttgcat aaatgctgca atgtgctcta
    31321 acccttccag attcagtttc accgcgtatg cccagcgcag aaccgtaaac agataggcat
    31381 cagcaattgt aaatctttgc ccgcagatcc agtgctcatc cttcagtgcc tcgttcacat
    31441 attgcagctt cttctccagc tgcgcgcgaa ctgtcggttt gtactcttcc ggtgtatcag
    31501 ggcgaaacag aggtgtgaaa cctttatgca gctcggtggc gatgtaattc agccattcga
    31561 tggttttata gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg gggacgctgt
    31621 cggcaagata ctgcataatc gctacgcctt ccgtcagcaa agtaccgtca tccagcagca
    31681 ctgcaggcac ctgcccctta gggttaacga caaagtaatc gtcaccgttt tcgagacgtt
    31741 ttttcattaa atccacactg acgagggtaa aatcctttcc gctctcacgc agggtgatat
    31801 gggaagcgag agagcaggca cccggtttgt agaacaattt catcggtaac tccttttgtc
    31861 tgtggtttaa agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa cgaaaagtta
    31921 gcggcttgta aaagagtttc cctgaatttt acgctacagc agctttcgcc gctttgtctg
    31981 cagcgtcatc ctgcgtcata cggtgcagtt taggcgcggt cagcagcatc agtactgcaa
    32041 taatggcagt agcgacacca atctgcaaga atacgcgacc ataaacttcc agtgacatca
    32101 gcgggtcggt aacgttatcc ggcacagcca tcatacccgc aacataacca ccaatcaggt
    32161 ttgcaccggc agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa
    32221 cgagttgtgc aaccattgcc agacccagac cagagatcat cagttccccg atgctctgca
    32281 ggccatagct tgcgaccagc cagcttacag acacgatacc agcgtcagac gcgaatttcg
    32341 cacccagcgg cagaatcagg aacgcaccag aacacatcac catgccgatt gcaaacttgg
    32401 ttggcatcgg cagggtatcg cccatcttgt tatagatagc ggccagaatc ggactaccga
    32461 tgatgatcca gaacgggttc agtgcctgat actgctcagg ttctacggcc agacccagaa
    32521 tggagtgctc aacgttacga atcgcaaaga agttcagtga cgttggcatc tggctgtaca
    32581 gcacgaagaa gataatggct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg
    32641 cagcaccttt catcgcgaag gcttctttac cgaagataac cacgataccg aaggcaacaa
    32701 cgcccagcgc catacgcgca acttcctgat tgtgcagcag ccaggtggcg atagcgatca
    32761 gtgccacaac accaataatg gtcagcagca ggttacggta gttgataggc tcgaagtctg
    32821 gttttgaacc gtactgttta acccagcgtt ggcagaaggc gaagttaacg atagtgatca
    32881 gcaggcctac aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg gccagccacg
    32941 gcgtagcaat catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg
    33001 caccgtccag acgcgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag
    33061 ctttaaacag gccgttaccg accgcaatag ccgccatacc catataaacg ataccggcgt
    33121 cgtgaccaga ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa
    33181 ttacgcgttt agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat
    33241 aaaccagggc actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt
    33301 gtttaaccag gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata
    33361 actcaatcga gaagatgaga tagaacgcct tcggttgttt gaaagcgttc aaactgacgc
    33421 tttcagttgg tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta
    33481 acgggggtgc tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg
    33541 ggaaacggca ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg
    33601 ttacatataa ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt
    33661 tttcaaaaaa cgtaacggca gattttttca ctgcactgag aggggggtga tggcttaacg
    33721 gcgatatgtt ctgctattta gcgtttttac tagtgagata gtccagtttc tgaaaaatag
    33781 ccagtggaat gttttgtagg tcaatatttg ttgcgtgtgt aaatttaaat gattttaacc
    33841 tgattcttgc aatgttttta cattgcgaca gcatcgtgat cttgatcacg aatgaataga
    33901 aaatttcgct attaaaaaaa caattaacct gcatttttgg ttatccgcaa acggattacc
    33961 tgatgataac gctaagcaat ggcatcattc acgttaaaaa gaagttttaa aagaggataa
    34021 agaaaggtta tcaatggggt aatcggtgtt accccttttc ttcagatgtc aactttctct
    34081 ttgtattcac aaagatcttc aataatacaa gagccacagc ggggcttgcg ggcaatgcag
    34141 gtataacgcc cgtgcaggat caaccaatgg tggcagtcga ctttaaactc tgccggaacc
    34201 actttcagta gcttttcttc tacctgttcg acgtttttcc ccggcgcaaa ttgagtacga
    34261 ttacaaacgc ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgaa tgcagtgttt
    34321 aatacgacgt tggctgtttt acgacctacg ccgggcaggg cttcaagcgc agcacgatct
    34381 tccggaacct cgccattatg ctgctccagc aagatacggc aggttttgat gatattttct
    34441 gctttgctgt tataaagccc aatcgttttg atataggttt tcaccccttc aacgcccagt
    34501 tcaagcatcg ctgcaggcgt attcgccacc gggtagagtt tcgccgtcgc cttattaaca
    34561 ctgacatcgg tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa aggcgaactg
    34621 aaattaagct cggtggtggg atgaggattg ttctcacgca ggcgagtgag gatctccagg
    34681 cgttttgctt tgttcatcag acattccctg tttcaccgtt tggcaatgca cgttcggcag
    34741 ctgcttcagt acggcgcttt ttcattcttt catcaatcag gtattttcct gccagcatca
    34801 gtcccaggcc aataaatgca cctggaggca gcatcgccag caggaaaggg gagtcggtgt
    34861 ggaaaatttc cacgcgtaat acttttgccc agctacctaa cagcgcatct gcaccgtcaa
    34921 acaatgtgcc attgccgata atttcgcgta gtgaacccag cacgaacatg gcgcaggttg
    34981 cgcccatacc aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga
    35041 aggcttcagc gcggcccaca acgatacagt tagtgacaat cagcggaata aaaatcccta
    35101 atgattgata caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg ctgaccaccg
    35161 aggcgatgat catcacgtaa atgggaatgc ggatctcggc tggcgtccag tgacgcagcg
    35221 tcgaaatggt caggttggtc agcgtcagta ccagcgtagt cgcaagtcct aaacccagag
    35281 cgttagtggc agtggacgtg accgccaaca gaggacaaag gccgagcaac tggaccagcg
    35341 cagagttgtt tttccacaac ccctgaacaa taacgtcttt aatttcgctc acgctttatt
    35401 ctccacaggc aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc
    35461 gttttaccgc attaaccacc gcgcggggag taatcgtcgc gccggtgaac tggtcgaaat
    35521 caccaccatc tttcttcacc gcccagtgcg catcatctgc accactgatt tttttacccg
    35581 caaaatgggt gatccagtca gaaaggcgca gttcgatttt atcgccaagc cctggcgttt
    35641 cgtggtgctc tgtcacgcgc gtgccaagta ccgtgccgtt aaaatcggct cccaccagca
    35701 gctgaatcgc accggaatag ccatctggcg cggttgcttc cagaacggcg gctaccggtt
    35761 tgtcatcctg tttggcgatg taaacccgat gctcaccttt acctaactct ggcgcagtta
    35821 ccagatagca actctgtgcc agcgcattgt tatagcgttc ggctggcagc acctgatcaa
    35881 ataatgcctt ttgttgcaga ctggcctgtt cagcaatcgt cgttttggtc atctggttga
    35941 tggccgcagt taaccctgtt gaacccgctg caaatagcgc taacgtaatg ccgtgttttc
    36001 ggatagtttt cagcatggtt taccctttgc gatggccgta gacgcgcgga cgcgtgtagt
    36061 aatcgatcag aggaaccgtg atgttcgcca gcaggacggc aaaagccacg ccgtcaggat
    36121 agccgccgaa actgcggatc aaccagacta ataagcccgc aagcgcgccg aaaataagac
    36181 gaccacgatt ggtcgtagaa gcggtaaccg ggtcagtcaa aataaagaat gcgccgagca
    36241 tggtcgcacc agacagcaga tgaatttgcg gtgctgccag tgtttctggt gagaacaacc
    36301 agcccaacgt tgcgcataac gccagcgtta ctaagaagct gaggggaata tgccagcgaa
    36361 tcgctttctg ccatagcaac cacaggccgc cagccagcca ggcgagattt acccattgcc
    36421 aaccagcgcc cgccagaata ccgctgtaga tcggatattg cataatctgt tcaaccgaat
    36481 gaccggcacg gacagaggtt ttaaatgtat ccagcggtgt cgcctgacta atgccatcaa
    36541 tacctaagcg tagtgtgttc atatcaccac cactggcggt atgcccgctg aaaataacct
    36601 gtatggcgtc gataaaacca gggatgttga ccgcaatttc atgtggcggt aaccagctgg
    36661 tcatctgcac cgggaaggag atcagtaaga ccacataacc aatcattgct ggattaaacg
    36721 ggttttgccc cagaccgcca tacaactgtt tagcgatgat caccgcaaac accgtaccca
    36781 gcacgaccat ccaccatggc gcgagggggg gaatacttac cgccagcaat aagcctgtca
    36841 gcaatgctga gttatctttc aacgttgcgg ctaccgactg cttgcgtagt ttgagtacga
    36901 gagcttcggc taacagagca ctaaccgatg ccaacaggat ctgaacgaga gtaccccaac
    36961 caaaaaacca cagttgcgct gcgattcctg gcacggctgc gagcaacacc aacagcataa
    37021 tgcgcgatgt ctggcgctgg ttatgggtat aaggggagct agctattctg aataccattt
    37081 agtcctcgtt tacaaccttc tgctgggcgg cttttttggc ctgagcgcgg gcaatagccg
    37141 cggcaactgc cgctttgcgc ggatcaacct gttcttctgg ttctgcgtta gtctgttgct
    37201 gttccagctt gcgcgctttg gcacgggcaa tagcggcttc gacggcggct ttgcgcggat
    37261 caacctgttc ttctggttcc gcattagcct gttgctgttc cagtttgcgc gctttggctc
    37321 gggcaatagc ggcttcgacg gcggctttgc gcggatcaac ctgttcttct ggttccgcat
    37381 tagactgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
    37441 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
    37501 gcgctttggc acgggcaata gcggcttcga cggcggcttt gcggggatcg acctgttctt
    37561 ctggttccgc attagcctgt tgctgttcca gcttgcgcgc tttggcgcgg gcgatagctg
    37621 cttcaacggc agttttacgt ggatcagcaa cggttgctgc gtcgttagtt tgctgcagtt
    37681 ctgcctgttt cgctctggct tgcgctttac gggcttcccg tgctgcaata attgcactgt
    37741 tatccgggcg ttcgcccgct ttaatcacaa taggctgtgt agcctgggcc tgtttctctt
    37801 tcacccgcgc cagagcggca gcaatcgcat ctttatcttt ggctgcaggt tgaacggctg
    37861 cgctcttatg tcgttcaagg cgagccgctt tttcgcgctc cagacgagcc tggcgcgctt
    37921 cgaaacgcgc tttggcttct gcggcgcgct tttcttcctg acgaatagcc gcaatttcag
    37981 ctttttcctg acggaaatat tgcaccaggg gaatattgct cgggcaaacc cacgcgcaag
    38041 ccccacattc aatgcaatca gcaatgttat gcgtggtagc tttatcgtgt tgctgacctt
    38101 tgctgaacca gtacaactgt tgcggcaaaa gatccgcagg gcaggcgtca gcacaggcgc
    38161 tacaccggat gcagctttgt tcttcctgtg gttcgccaag ttcattggca gagggagcca
    38221 acagacagtt ggtaatcttt acgaccggga catccagcca tggcaaggta aagcccatta
    38281 gcgggccacc cataatcacc atttgatcgg cagaggggca gaatccggca tcattcaata
    38341 aatgacgcac tggcgtcccc agccgtgccc agacgttgcc cggacgagcg attgcttcgc
    38401 cagtcagggt tacaacacgc tcggtaatcg gctcgccatc aataacggca cgtttcactg
    38461 cataagcagt gccgacgttt tgcattaata cgccgatatc ggatgaacgc ccgccatgtg
    38521 gaacctgctt cccggtcaga atgtaggtta attgtttagc accgccagaa ggatatttgg
    38581 ttggaatcac ccgcagagaa atatcgttag agtccgccag caccgcgcgc agcatggaaa
    38641 tcgcctgcgg tttgttatct tcaatgccga taagaatttc gcgtggctgc agaatatgcg
    38701 caagaatgcg aataccctct acgacctgag ccgcgcaatc ctgcatcaaa cggtcatcgg
    38761 cggtaatgta cggctcgcac tcagccgcgt tgataatcaa cgtttcaatc ttatctccgc
    38821 caccctgcaa tttaacgcct gtcgggaatc ctgcaccgcc cagcccggct acaccaaact
    38881 gatgtatgcg ctcgattaac tcttcgcgac tgcgagtgcg ataatcggcc cagccgtcgc
    38941 gcgggatcca gcagtcttca ccatcggcat caataatcac gcttaattca gctaaagctg
    39001 aaggatgagc cgtagagtgg ggcgcaatag ccgtaacggt acccgaggtg ggcgcgtgaa
    39061 caggcagcat tttgccgcga ccacgggtaa gcggctggcc gcgcaatact ttatcgccga
    39121 cgctaacgca caactcacct tcagcgccaa tatgctgttt cagtggaata acaaaacgct
    39181 gcgccagtgg tacctggcgc aggggtgtac cgttggactg ggttttcatc tccggtggat
    39241 ggatgccgcc gttgaaatcc cagattttat tttttctgaa tgcagagaat aacttaagca
    39301 tggtgttcca cgggaatgat acgcacggga atggtgttca gatcccattt ccaggagtca
    39361 ggtgtttctg cgaccggttg caacgagatg cagtgcgtcg ggcacggatc aacacataaa
    39421 ttgcagcccg tacagagatc actcattacc gtatgcatgg cacgggtagc gccaacgatg
    39481 gcgtctaccg gacaagcctg aatacattta gtgcagccaa tacagttatt ttcatcaata
    39541 accgccacca tccgcgcagg cgttagctct tgcgcttcgc catccagcgg ctgcggctcg
    39601 acattaagca actcggcaat ttttagcatc acagcttcgc cacctggggc gcaacggttg
    39661 attttttcac cgttacagct gatggcttcc gcgtaggggc gacagccggg ataaccgcac
    39721 tgaccacact ggctctgcgg taagatttcg tcaattttct caacgaccgg atcgtcttcc
    39781 accgcaaaac ggcgggaggc ataacccaga atggcgccaa acgccaggcc cagcaggctc
    39841 acggcggcaa cggcaatcca gatagcattc attacaactt caccaaacca ctaaagccca
    39901 taaaggccag agacataaga cctgcggtaa ttaacgcaat ggcattaccg cgaaaaggtg
    39961 ccgggacatc agccacagca aggcgttcgc ggatggcggc gaagagcacc atcaccagcg
    40021 agaaaccgac agcggcggaa aaaccgtaca gcgccgactg caagaaattg tgcccgagat
    40081 tgatattcag caacgccacg ccgagcactg cacagttggt ggtgataagc ggcaaaaaaa
    40141 tccccagcag acggtaaagc accgggctgg ttttgcgcac caccatctcg gtgaactgca
    40201 cgaccacagc aatcaccaga ataaatgcca gggtgcgcag gtaaatcaga ttaagtggga
    40261 tcaaaatcca cgtatcgata agccaggcgc aaatagacgc cagcgtcatc acaaacgttg
    40321 ttgccagccc catgcccatc gcggtttcca gttttttgga aacccccata aacggacaga
    40381 gaccgagaaa cttgaccagt acaaagttat tgaccagtac agttccgaca aacagtaaca
    40441 ggtagtcagt catttttcgg cctgaaataa aaaagccgcc tattatccgt taatccagag
    40501 caggcgacaa caggttaact gtaaggttat tacggattca caaacgtcgc ttttacccgc
    40561 gacgagcgtt tgaaataggg gacgatcaat gcggttgcca acaaagtaaa cagcaactgg
    40621 cgaacggcta tgccatcttc cacgggtgaa aaagcgaatg cttttactgc cagcaatacg
    40681 gaaataagca gccagataat atagtgttta ggaacacaac gccgacgttt aaagaaagcg
    40741 atggtcaacc acagtgtgta ataccacatg gcgatagcgg taataaaaga aacgccccat
    40801 aagatttgtg tcgtcagtgc ttgtccgccg agcgtttgaa atgtttgcgg agaagataac
    40861 gcagcggtat acagcaacaa cgccagcgtc gtactcaaca gagcgactaa aagccaggcc
    40921 aacgggccaa gcaaccagcc tccaatacgt tgtggcgttg tggtggtcat aggttctcca
    40981 gaatagcgac gcaaacaggc aaatctaatt ttaggggcgt tattataagg cttttataac
    41041 cgatcgctac tttctttatt ggacatagtg ccagacggac ttcggtacct ggccgaggtc
    41101 aaacaaacgc ccgccagaaa ccaactctgc gcgacggtga tcggcagcac gatacatatt
    41161 gatcagttcc tgatcgtccg tcagggtgta attaagatgg tcgtagagtt tttccagact
    41221 ttcgagtgaa ctgattttgc gaaattttaa taagtagtcc tgaacagtca taaaaggtcc
    41281 ataaaataat taataccaaa ggggtaggta ttattagtga tgataaaatt tcaccaaaag
    41341 tgaacgcttg tgttattaca ataataagaa tgcgctgatg gcaaatattc agggatgaaa
    41401 aactgctctg aaaagagtaa cgcgctttac tatccctgac aatcaccaac aacatcgaac
    41461 aagataataa attcctggtt taatatccga caagtgaaaa catgcacccg gacgggcagc
    41521 atgtcgctcc acaagtgcag agcttacttg tgttgtaccg aagcactctg ttcaggtggc
    41581 tgatagttgt caatgtgact cgccacgcca agaagaatga ctgaaacgac aagaacgatc
    41641 caacctgtta attcaataag acgattcatt acagcccaca tctctcttga ttgatccatt
    41701 aacttcaggg ggtaaatgtt acttagcaat aatagctcca gcaagatttt tactgaggtt
    41761 tttgcgatat ttagcttttg tcgttggaaa attcgctatt tttttgactt aagttaaaca
    41821 acatccctta ttgctggcag gttattaaac tgttgagcgt gggtaaagga tagtgtcaaa
    41881 tagccatcat acttcaatga gaggcaatga catgagcgac aacatccgtg ttgggttgat
    41941 tgggtatggt tatgcgagca aaaccttcca tgcgcccctg attgcgggca cgcccgggct
    42001 ggaactggcg gtaatctcca gcagtgatga aacaaaagta aaagccgact ggccaacggt
    42061 tacggttgtc tctgagccga agcatctgtt taacgatccc aacatagacc tgattgtcat
    42121 tcctacaccc aacgataccc atttcccgtt agccaaagcg gcgcttgagg cgggtaaaca
    42181 tgtggtcgtt gataaaccct ttaccgtgac actgtcacaa gcgcgagagc tggatgcgct
    42241 ggcaaaaagc ctggggcgtg tgctgtctgt attccataac cgtcgctggg atagcgattt
    42301 cttgacgcta aaaggtttac tcgcggaagg cgtgctgggt gaagttgctt actttgagtc
    42361 tcattttgac cgcttccgtc cgcaggtgcg cgatcgttgg cgtgaacagg gcggcccagg
    42421 cagcggtatc tggtacgatt tagcaccaca tcttcttgat caggccatta cgctttttgg
    42481 tttaccggtc agcatgacgg tagatttggc acagttacgg cccggagcgc agtcgaccga
    42541 ttatttccac gccatcttgt cctatccaca gcggcgagtc attttacacg gtaccatgct
    42601 ggcagccgct gagtcagcac ggtatatcgt gcatggatcc cgaggcagtt atgtgaaata
    42661 tggcctcgat ccacaggaag aacgtctgaa aaatggcgag cgtctaccgc aggaagactg
    42721 gggctacgat atgcgtgatg gcgtacttac ccgcgtggaa ggtgaggaac gtgtcgaaga
    42781 aacgctgttg acggtgcctg ggaattatcc ggcttactat gcggctattc gtgatgcgtt
    42841 aaatggcgat ggtgaaaatc cggttccggc aagccaggca atccaggtaa tggagttgat
    42901 tgagctgggc atcgaatccg ccaaacatcg cgcgactttg tgccttgcat gaaaaatgga
    42961 tggtgcaatt tgcaccatcc attttgttac ttcgcggcga ctttttctcg cagtgcgcgt
    43021 ttttcctcag cgctgaggaa agccatttcc agaccattaa tctgtgcctg gcggatttgc
    43081 tcgcgggata acccagcagc tggcgcggca acggtatatt cgtgaatgat atccactccc
    43141 tgtacgccgg gatcgtcagt gttaatgctg gcacgaatgc catgctcaag gaacgttttc
    43201 agcggatgtg cagccagctc tgctacggtg ctggtctgaa tattggaggt cagacaggat
    43261 tcaataccaa tttgttgctc ggcgagaaaa tccatcagcg cccgatcttc aatggctttt
    43321 acgccatgtc caatacgctc cgcacccagt tcacgaatcg cctgccagat gctttccggc
    43381 ccggcagctt cgcctgcatg gacggtaata tgccagcccg catcacgcgc gcggttgaag
    43441 tgagaaagga acagacttcc cgggaaacca agttcatcac cggctaaatc aagtgcggta
    43501 atctggtcac ggtgggctaa aaaggcctcc agctcttgct gacaggcggc ttcgccgaag
    43561 gtccggctca taatgccgat aagcttcgcc tgcacaccaa aggtgcggca accttcacgt
    43621 acgccatcga tcaccgcttc gacaacaccc gctacaggca gctgatgtgc cattgccatg
    43681 tagcctggtg aaaaacgcag ctcgacatag tgcaggccgt gacgggctgc atcttcaatg
    43741 ttttcaaatg ccacgcggcg acaggcatca agagaggcga gaactttaac gccccagtca
    43801 agtttggtca gaaagctcac cagatcgggt tcgttggcaa tgacctgaac gtggggaatc
    43861 agtgtttcca gggattgtgc aggaagcgag atattatact ggcggccaag ttcaagaatg
    43921 gtctggggac gaatgttgcc atcaaggtgg cgatggatat cagttaatgg cagggtggta
    43981 tcaatcatgg tcgcactctt tttctgaata taaaagtgcg caccattata aaaacaaagc
    44041 agggtaaaaa tccacgattg cgcaacgatt ttttacgttg cgcaattggg gttagcgaac
    44101 agcgcggatg gcgtttatta atccagccac gcctttttcc agtttcgaac gtgggcagcc
    44161 ggcattgaga cggacaaaac cacgaccttc ttcaccatag gtataccccg gcatgatcgc
    44221 gactttttct tgctcgataa gcgctttttg caacgcgttg tcgtcaatat tcaacggacg
    44281 tagatcaagc caggccagat aagtggattg cgggatctgc cagttgagtt caggaaacgc
    44341 ggcgttcatt ttatctgaga tatacgtcag gttatctttc agatagacgc gtaaggcatc
    44401 cagccacggt gcaccttgct gataggcggc gatatgggca gttaacgcca gtaccgaagg
    44461 ggaagaaagc ccatcacgtc ctttcagtgc cgataaatag gcatcacggc tgctgctatt
    44521 ttctataatc ccgtaagcac cggtcagcgc gggaatattg aaacttttcg agcccgacgt
    44581 cagcaacgcc cagtctcccc gcgccacatt actccaggga atatgcggct gctcgcccca
    44641 gaccatatcc atatggattt catcggaaat aacccgcaca ccatgacgct cgcacaggtc
    44701 agccatgatc tccagctcat cgcacgtcca cactttcccg gtaggattct gtgggctaca
    44761 caggagcata attttacatt ctggtttcgc caacacggct tccagcttgc ccatatcgca
    44821 aaaccagtcg tcagcctgct tctctaaagc aacgggcatt actgtgcgct ggttaccttc
    44881 aatggccttg taaaatgcgt cataggcggg cgtgtggatc accacgcctt cacctgtttc
    44941 agaccactga cgaatcagtt ctgaaaccat atagatgaca gaagggccat acaccaccgt
    45001 ctgcgtatca attgcggtgt aatgctgggt ggaaaaccag tgggcaatag ccgcgagaaa
    45061 ctcatcgttt ttccagcggc tgtagccaaa tacgccgtgc atcaggcgct gattcagcgc
    45121 ctcgataatg cagggggcag tggcaaaatc catgtctgaa atcgtgaacg gtaacaggtc
    45181 agcagtgccg aaacggtcag cgacataatc ccactgtgta caccatgtgc catgacgatc
    45241 cacgaccttt gaaaaatcga acatatctta tccttatgcc tggacagtat gcatcagacc
    45301 ggccatttca tcttttactg actgaacttg tggcccgata acaacctgca ggttatgttg
    45361 attaagttgt actacgccaa ttgcccgatt gtccttcagt gcctgcacat taacaagcga
    45421 catatctttc acagacaaac gcagacgggt aatgcagtta tcgaggctga caatattgtc
    45481 ggcaccgcct aatgcttcga ggattgcagg aacgttgtaa cctgatttac ccggcgcacc
    45541 ggcaacggct ttttcgattg agctggcaac ttcgctatcg cgccccgggg ttttcagatt
    45601 gaagcgggtg atagcgaaac ggaagatgac gtagtaaacg acaaaccaga ttgccgccac
    45661 cactggcacc atgtaccact tggttgacag accatgcaaa ataccgaaca ccacgaagtc
    45721 gatgatattg ccgtcggtat taccgatggt gacgccgagc acagacatga cggtgaagcc
    45781 gaggccggtt aacagcgcgt ggatgacata cagaactggc gctacgaaca ggaacaggaa
    45841 ttccagcggt tcggtagtgc cgccaacgac gcaggcgatc aggccagaaa tcagcagacc
    45901 tttaatttta tggcgatttt ccgggcgcgc gcagtgatac atagctaacg ctgcacccgg
    45961 cagaccgccg agaaacgcag gcattttacc ttgcgaaagg aaacgcgtgg cgctttcaga
    46021 aaaaccgtga gtggtcgggc aactcaattg cgcctggaag atggtcagtg cgccgctgac
    46081 ggtttgaccg cagacttcct gcgtgccgcc tgcgtcggta aagcgaatta atgctaccag
    46141 aatgtgatgc agaccaaacg gcaacagcag acgttcaccg gtaccaaaca gcatcggtcc
    46201 gaaatcaccc gcgctgttta tcatatggcc caagccgcta atacccatgg cgaaaatcgg
    46261 ccagactaat ggaatcacca ggccgacaag gcccatcacc agcgaggaga taattggtac
    46321 gaagcgcgta ccgccgaaga atgccagcgc atccggcagg cggatattat ggaaacgctc
    46381 atgcagcatc cagacgataa taccggcgat caccgcaccg aggatcccgg tatcgatcga
    46441 ctggatccca agaatgctct ggatgttatt ggctttcaga accgcggcat ccgtggttgg
    46501 cagaatgcct ttattggtca accagaagtt taccgcgagg ttcattaccg cataaccgac
    46561 gaagccagcg aatgccgcta cgcctttatt ttcgcgtgcc aggcccagcg ggatggcgat
    46621 acagaacatg acaggcagga aactaaaagc aaacgagcca atcttactca tccaggtaaa
    46681 gatagcctgc aacacggggt tgcccaggac cgggatcagg gttatgacat catggctgct
    46741 aagagaacta ccaatgccga gcataatgcc gcagaacgac aataatgcca cgggtaacat
    46801 gaaggttttg cctaactgct ggaagaactc ccacagcgtg actttcggtg ctgttttcgc
    46861 cgtcataaaa cgactcctcg tagagaacaa tctggattca gtaaattgcg cgagaagata
    46921 aaacgtttta tcaaatttta gtgaggcata aatcacatta cgcaacgata atagcgggta
    46981 taagataaat aaaaggtaaa acgttttatc tgtcacataa tcagggagta ggtcatctgc
    47041 atggctaccg ccaaaaaaat aaccattcat gatgttgcgc tggctgcggg cgtgtcggta
    47101 agtaccgttt cgctggtgct tagtggcaaa gggcgaatct ctaccgccac aggagaacgc
    47161 gttaacgccg ccattgaaga gctgggattt gtgcgcaatc gccaggcgtc ggcgctgcgc
    47221 ggcgggcaaa gcggcgtcat tggtttgatc gtccgtgatt tatctgcgcc gttttacgcc
    47281 gaattgacgg ccggattgac ggaagctctg gaagcgcagg gacggatggt ttttttgctt
    47341 cacggcggta aagacggcga gcagctggca cagcggtttt cactgttact gaatcagggt
    47401 gtggatggtg tggtaattgc cggggctgca ggaagcagcg atgacctgcg acggatggca
    47461 gaagaaaaag ctatcccggt gattttcgct tcccgtgcca gttatcttga tgatgttgat
    47521 acggttcgcc cggacaacat gcaggctgca cagttgttga cggagcatct cattcgcaat
    47581 gggcatcagc ggatcgcctg gctgggaggg caaagttcct cattaacccg tgcagaacgg
    47641 gtggggggct attgtgcaac tctactaaaa tttggcctgc cgtttcacag cgattgggtg
    47701 ctggagtgca catccagcca gaagcaagcc gcggaagcta tcacggcgct tttacgtcat
    47761 aacccgacca tcagtgctgt ggtttgctat aacgaaacta ttgcgatggg ggcatggttt
    47821 ggtttgctca aagcagggcg gcaaagcggg gaaagcggag tcgatcgtta ctttgagcaa
    47881 caagtttcgc tggcggcatt taccgatgcg acaccaacca cacttgatga tatacccgtt
    47941 acctgggcca gcacgcctgc gcgggaactt ggtaccacac ttgcggatcg catgatgcaa
    48001 aaaatcaccc atgaagagac gcattcacgc aatcttatta ttcccgcccg gctcattgcg
    48061 gcgaaataat cctctcttta tctgctatac ctggtagtgt cctttcctca aggttaatga
    48121 taaaaaatgt agcacacagg cggtgccttt tatttttact gaagttcaca ggaggtttat
    48181 gtgtttaatt ctgacaacct gagactcgac ggaaaatgcg ccatcatcac aggtgcgggt
    48241 gcaggtattg gtaaagaaat cgccattaca ttcgcgacag ctggcgcatc tgtggtggtc
    48301 agtgatatta acgccgacgc agctaaccat gttgtagacg aaattcaaca actgggtggt
    48361 caggcatttg cctgccgttg tgatattact tccgaacagg aactctctgc actggcagac
    48421 tttgccgtca gtaagctggg taaagttgat atcctggtta acaacgccgg tggcggtggt
    48481 cctaaaccgt ttgatatgcc aatggcagat tttcgccgtg cttatgaact gaatgtgttt
    48541 tcttttttcc atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg cggtggcgtt
    48601 attctgacca tcacttctat ggcggcagaa aataaaaata taaacatgac ttcctatgca
    48661 tcatctaaag ctgcggccag tcatctggtc agaaatatgg catttgacct gggtgaaaaa
    48721 aatattcggg tgaatggcat tgcgccgggg gcaatattaa ccgatgccct gaaatccgtt
    48781 attacaccag aaattgaaca aaaaatgtta cagcacacgc cgatcagacg tctgggccaa
    48841 ccgcaagata ttgctaacgc ggcgctgttc ctttgctcgc ctgcagccag ctgggtaagc
    48901 ggacaaattc tcaccgtctc cggtggtggg gtacaggagc tcaattaata cactaacgaa
    48961 ccggtaaaca gtcgtatgtg ttgtttaccg ggatagcgtg tgcaattcat caacgtctct
    49021 gctaaacaac tggcagccaa atcacggcta ttggttaacc aatagctgga tgaaaagcat
    49081 acgaatagag tgtgccttcg cactattcaa cagcaatgat aggcgctcac ctgacaacgc
    49141 ggtaaactag ttattcacgc taactataat ggtttaatga tggataacat gcagactgaa
    49201 gcacaaccga cacggacccg gatcctcaat gctgccagag agattttttc agaaaatgga
    49261 tttcacagtg cctcgatgaa agccatctgt aaatcctgcg ccattagtcc cgggacgctc
    49321 tatcaccatt tcatctccaa agaagccttg attcaggcga ttatcttaca ggaccaggag
    49381 agggcgctgg cccgtttccg ggaaccgatt gaagggattc attttgttga ctatatggtc
    49441 gagtctattg tctctctcac ccatgaagcc tttgggcaac gggcgctggt ggtcgaaatt
    49501 atggcggaag ggatgcgtaa cccacaggtc gccgccatgc ttaaaaataa gcatatgacg
    49561 gtcactgaat ttgttgccca gcggatgcgt gatgcccagc aaaaaggcga gataagccca
    49621 gacatcaaca cgtcaatgac ttcacgttta ctgctggatc tgacctacgg tgtactggcc
    49681 gatatcgaag cggaagacct ggcgcgtgaa gcgtcgtttg ctcagggatt acgcgcgatg
    49741 attggcggta tcttaaccgc atcctgatcc tctcatttcg cgggctggtg ataactgtgc
    49801 ccgcgtttca tactttaatt tccctgtaca aaatacaatc tagccgggat cggagaattc
    49861 cccccaaaaa tattcaccgt agccatatgt catgagagtt tatcgttctc aagatgctcg
    49921 aacgaacgtt cgattgctta ttttatggct tctgtcaacg ctgttttaaa gattaatgcg
    49981 atcaatatca cgctgtgggt attgcagatt ttggtttttt gatcgcggtg tcagttcttt
    50041 ttatttccat ttctcttcca tgggtttctc acagataact gtgtgcaaca cagaattggt
    50101 taactaatca gattaaaggt tgaccagtat ttttatttga atgaggagtc ccttatgtta
    50161 cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt
    50221 ctggatcgcg aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc
    50281 cgggcaattg ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat
    50341 tatgtgggca acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag
    50401 cgtatcgtgc tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag
    50461 gaagtgatgg agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt
    50521 attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact
    50581 atcccgccgg gaatggtgat taccgatgaa aacggcaaga aaaagcagtc ttacttccat
    50641 gatttcttta actatgccgg gatccatcgc agcgtaatgg tctacaccac gccgaacacc
    50701 tgggtggacg atatcaccgt ggtgacgcat gttgcgcaag actgtaacca cgcgtctgtt
    50761 gactggcagg tggtagcaaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcagcag
    50821 gtggttgcaa caggacaagg taccagcggg actttgcaag tggtgaatcc ccacctctgg
    50881 caaccgggtg aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt
    50941 gatatctacc cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg
    51001 attaaccaca aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttgcgt
    51061 ggcaaaggat tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg
    51121 gccaactcct accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat
    51181 gaacatggca tcgtggtgat tgatgaaact gctgctgtcg gctttaacct ctctttaggc
    51241 attggtttcg aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg
    51301 gaaactcagc aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac
    51361 ccaagcgtgg tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg
    51421 gaatatttcg cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc
    51481 tgcgtcaatg taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg
    51541 ctgtgcctga accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag
    51601 aaggtactgg aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc
    51661 accgaatacg gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt
    51721 gaagagtatc agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc
    51781 gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc
    51841 gttggaggta acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt
    51901 ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa
    51961 caatgaatca acaactctcc tggcgcacca tcgtcggcta cagcctcggt gacgttgcca
    52021 ataacttcgc cttcgcaatg ggggcgctct tcctgttgag ttactacacc gacgtcgctg
    52081 gcgtcggtgc cgctgcggcg ggcaccatgc tgttactggt gcgggtattc gatgccttcg
    52141 ccgacgtctt tgccggacga gtggtggaca gtgtgaatac ccgctgggga aaattccgcc
    52201 cgtttttact cttcggtact gcgccgttaa tgatcttcag cgtgctggta ttctgggtac
    52261 cgaccgactg gagccatagc agcaaagtag tgtatgcata tttgacctac atgggcctcg
    52321 ggctttgcta cagcctggtg aatattcctt atggttcatt tgctaccgcg atgacccaac
    52381 aaccacaatc ccgcgcccgt ctgggcgcgg ctcgcgggat tgccgcttca ttaacctttg
    52441 tctgcctggc atttctgata ggaccgagca ttaagaactc cagcccggaa gagatggtgt
    52501 cggtatacca tttctggacg attgtgctgg cgattgccgg aatggtgctt tacttcatct
    52561 gcttcaaatc gacgcgtgag aatgtggtac gtatcgtggc gcaaccgtca ttgaagatca
    52621 gtctgcaaac cctgaaacgg aatcgcccgc tgtttatgtt gtgcatcggt gcgctgtgtg
    52681 tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt ctacgtgcgc tatgtgttaa
    52741 atgataccgg gctgttcact gtgctggtac tggtgcaaaa cctggttggt actgtggcat
    52801 cggcaccgct ggtgccgggg atggtcgcga ggatcggtaa aaagaatacc ttcctgattg
    52861 gcgctttgct gggaacctgc ggttatctgc tattcttctg ggtttccgtc tggtcactgc
    52921 cggtggcgtt ggttgcgttg gccatcgctt caattggtca gggcgttact atgactgtga
    52981 tgtgggcact ggaagctgat accgtagaat acggtgaata cctgaccggc gtgcgaattg
    53041 aagggctcac ctattcacta ttctcattta cccgtaaatg cggtcaggca atcggaggtt
    53101 caattcctgc ctttattttg ggattaagcg gatatatcgc caatcaggtg caaacgccgg
    53161 aagttattat gggcatccgc acatcaattg ccttagtacc ttgcggattt atgctactgg
    53221 cattcgttat tatctggttt tatccgctca cggataaaaa attcaaagaa atcgtggttg
    53281 aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag cgatatcact aattaatatt
    53341 caataaaaat aatcagaaca tcaaaggtgc aactatgaga aaaatagtgg ccatggccgt
    53401 tatttgcctg acggctgcct ctggccttac ctctgcttat gcggtgcaac tggctgacga
    53461 tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc agggcggata agcccagtgc
    53521 tggcgcggga agagatattt acgcatgggt acagggagga ttgctcgatt tcaatagtgg
    53581 ttattattcc aatattattg gcgttgaagg cggggcgtat tatgtttata aattaggtgc
    53641 tcgtgctgat atgagtaccc ggtggtatct tgatggtgat aaaagttttg gctttgccct
    53701 gggggcagta aaaataaaac ccagtgaaaa tagcctgctt aaattaggtc gcttcgggac
    53761 ggattatagt tatggtagct taccttatcg tattccgtta atggctggca gttcgcaacg
    53821 tacattaccg acagtttctg aaggagcatt aggttattgg gctttaacac caaatattga
    53881 tctgtgggga atgtggcgtt cacgagtatt tttatggact gattcaacaa ccggtattcg
    53941 tgatgaaggg gtgtataaca gccagacggg aaaatacgat aaacatcgcg cacgttcttt
    54001 tttagccgcc agttggcatg atgataccag tcgctattct ctgggggcat cggtacagaa
    54061 agatgtttcc aatcagatac aaagtattct cgagaaaagc ataccgctcg acccgaatta
    54121 tacgttgaaa ggggagttgc tcggctttta cgcgcagctc gaaggtttaa gtcgtaatac
    54181 cagccagccc aatgaaacgg cgttggttag tggacaattg acctggaatg cgccgtgggg
    54241 aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg aatggtgccg tggtggatac
    54301 cgacattggc tatccctttt cattaagtct tgatcgtaac cgtgaaggaa tgcagtcctg
    54361 gcaattgggc gtcaactatc gtttaacgcc gcaatttaca ctgatatttg caccgattgt
    54421 gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt gaaggcgcgg gtatcttagg
    54481 tggtatgaac tatcgggtca gcgaagggcc gttacaaggg atgaatttct ttcttgctgc
    54541 cgataaaggg cgggaaaagc gcgatggcag tacgctgggc gatcgcctga attactggga
    54601 tgtgaaaatg agtattcagt atgactttat gctgaagtaa aaaataacgc cggagagaaa
    54661 aatctccggc gttttggctg tgaattactg ctgcggaata gcgggtacga ccggaacatt
    54721 taatgccagc attgcaaaca taccaacgaa atcttccagt ggcattttct gcccgtttaa
    54781 cgttatctga ccgttagtat attgcaggct ggtggtgatg gtattgtcct gcaaggtggt
    54841 cagacggaac atctgcccca ttgctgatgc accttcaact tgctgtttcg ccagtttttt
    54901 cgcttgatct tcctgataac cttccagctt cgctacctga gtcataaact cagttgccat
    54961 atccaccgga atggtcagtt tcgcatccag agatttaacc gaacgatcta cttcctgcgc
    55021 cagcgtttgc ggcgcttctt tagtcgttgc cggatctttc aggaacagcg acagattcag
    55081 cgcactttca ccctgactgt ttttccagct tagcggcgcg atagtaatca ccggatcgcc
    55141 tttcaacatc agcggcaggg cgctaaagaa ggcttccgtc actttctcct gataaagttc
    55201 gggattgttg gcaatttctg gctgtgccag cagcgcctga gtttgcgcgt tatattgctg
    55261 gctaaactga tgccaggctt caccatcaat ctggccgact tttaaagtca gcttgccgct
    55321 gcccagatcc tgattctgta ccttcaggct gtttagcgag taatccagtt ggctattgat
    55381 cgttttaccg tcattgacca gatccgattt accgttgatc tccatgcttt ccagcagtgc
    55441 cagttctttg ccttccactg aaatggtcat tttttccagt gacagttttt ggtttcctac
    55501 gcgctcacca aaacttgcca gcgtgctgga accgtcggtt ttcagattat taaaggtcaa
    55561 ctgcactttc tggttgtatt cgttaactgc gtctatccga ccactttgcg cctccccgga
    55621 aagggagatg gctttgccgt ctctgtcagc atttaactgg aactcgccgc cgctaaaggc
    55681 gactttttca tccttttgct cgtaattcag tggcttgagc gaaatatcgg aactggaatc
    55741 accgctgtaa ccaatgcgcg agttaatctc aaaaggcgtt tcaccttttg ccatatcaaa
    55801 cagtggtttg cttacttcgt tattaaccag cgtggtttga attgatgcca tcgacgggat
    55861 caggttcagt tttttaagct gggcaagcgg gaagggacca tgatcaaccg attcgttgaa
    55921 gatgacgctc tgaccgcttt taatccacgg attttctttc ccggcaatgg gtttcaccaa
    55981 cagttgcaac tggctgctga atacgccgcg atgatagttt tgataactca cttccaggtt
    56041 ggattcagga gctgtcagtt tgagttgcgc gttcgcctgc gcgaccatgt cttcgagatg
    56101 ggtttcaatc ttcttgcctg tataccatgc gccgcctgtc cagactacgc ctagcgcaac
    56161 aatgacgcct accgctacca gcgatttatt cataatgatt atccataaaa tgaaatcagg
    56221 cggactggcc gcctgaaggt gttataagcc tttaataagc ttagcaagag atgttaattt
    56281 tttcagtaag ctcttacagc ttgttgtaaa cacgcgctaa acggccgtgg cctttgacag
    56341 tcaccggtga ttcgttggcg gcaataaacg ctgattcacc cggtttaagc tgtaactgct
    56401 gagaaccttt ccacaacgtt gcatcgcctt cgacgcagaa caaaatggcg gcactctgct
    56461 ggctaatggt ggtttcttta tcactaaggt catgcagcga gaaggcaaaa tcatccactg
    56521 gaatcgggaa gtccagttct gcaccttgtt tcaccggctg ggtcaacaac tggttagccg
    56581 gtttggcttc gaatttcaca ttggcaacca gttccggaat atcaatgtac ttaggcgtca
    56641 gacccgcacg cagcacgtta tcggagttcg ccatcacttc cagcgccacg ccttgcaggt
    56701 aagcgtgcgg tgtttcagcg aacaggaaca tcgcttcgcc aggattcaat ttcaccacat
    56761 tcagtaacag cggggagaac aggccgctgt cttccgggta aaattcagaa attaaacgaa
    56821 tcgtttgcca cggttcaccc tgctgactgt ctagcgccga ttttaaaatc gccagcgcgc
    56881 gggatttttc ttcaccctgc atattcaaca ggctggcgaa cagttcgctt aaacgttcgg
    56941 cattaggctg ttgtaaaaag tgagcaattg ccggatgtgc acctgcgact ggctggagta
    57001 gggagacaat ctcggaaaac tcacgaaacg cgttcatcgc aaggaaaggc gtcagcgcaa
    57061 aaaccagctc cggcttgtgg ttaggatctt tatagttacg ctcggcggca tccatcggga
    57121 tacctgcggc attttctttg gcaaaaccga tttcagaatt gcgtttgttt ggatgaacct
    57181 gaatggagag cggctgtgct gcgcataata ctttgaacag gaaaggcagt tcgccaaagc
    57241 gtttggcaac ggcctctccg agcagagtcg atttattact ctcaatcaca tcacgcagtg
    57301 aaacgatatc tccggcggca ttctgcaccc gtgaactgct tttcggatgt gcgcccatcc
    57361 acagctcggc catcggctgg ctggacggat tttccatacc ataaagttca gtcaacgccg
    57421 ttttgctgcc ccaggcatag ttttgcactg agttaatgag tttttgcatg atcaatccct
    57481 gttttaatgt ggaaattaat cccgctatta aagcaagaat cctacgggaa gtaacctgga
    57541 gccgcaaaaa gtcgtactag tctcagtttt tgttaaaaaa gtgtgtagga tattgttact
    57601 cgcttttaac agggcaacgg aacacccgcc cagagcataa ccaaaccagg cagtaagtga
    57661 gagaacaatg tcaaacaaac cctttcatta tcaggctcct tttccactca aaaaagatga
    57721 tactgagtat tacctgctaa ccagcgaaca cgttagcgta tctgaatttg aagggcagga
    57781 gattttgaaa gtcgcacccg aagcgttaac tctgttggcg cgtcaggcat ttcatgatgc
    57841 gtcatttatg ctgcgtccgg ctcaccaaca acaggtggcc gacattctgc gtgacccgga
    57901 agccagcgaa aatgataaat atgtggcgct gcaattcctg cgcaactccg acatcgcggc
    57961 gaaaggtgtt ctgccaacct gtcaggatac cggcactgcg attattgttg gtaaaaaagg
    58021 gcagcgtgta tggaccggtg gctgtgatga agcggcgctg gcgcgcggtg tctataacac
    58081 ttatatcgaa gataatttgc gctactcgca aaacgcgccg ctggatatgt ataaagaggt
    58141 gaataccggc accaatctgc cagcgcagat cgatctttat gccgttgatg gcgacgagta
    58201 caaattcctc tgtatcgcca aaggtggtgg ttcggcaaac aagacgtatc tctatcagga
    58261 aaccaaagcg ttactaacgc cggggaaact gaaaaattac ctggttgaga agatgcgcac
    58321 gctgggtacg gcggcctgtc ctccgtatca tattgcgttc gtaattggtg gaacttctgc
    58381 agaaactaac cttaaaacag tgaaactggc ttccgctaaa tactatgacg aactgccaac
    58441 ggaagggaat gagcacggtc aggcattccg cgatgtggaa ctggaaaaag aattgctgat
    58501 cgaagcgcaa aatctcggtc tgggtgcgca gtttggtggt aaatacttcg ctcacgacat
    58561 acgcgtgatt cgcctgccac gtcacggcgc atcctgtccg gttggtatgg gtgtttcctg
    58621 ctctgctgac cgtaatatca aagcgaagat caaccgtcag gggatctgga tcgaaaaact
    58681 ggaacataat ccaggtaaat atatccctga agagctgcgt aaagcgggag aaggcgaagc
    58741 ggtgcgcgtt gaccttaacc gtccgatgaa agagatcctc gcacagttgt cgcagtatcc
    58801 cgtttctaca cgcttatcgc ttaacggcac gattatcgtc ggtcgtgata ttgctcacgc
    58861 caaactgaaa gagcggatgg ataacggtga agggctgccg cagtacatta aagatcatcc
    58921 gatttactac gcgggtccgg ccaaaacgcc ggaaggttat gcctccggtt ctcttggccc
    58981 aacgaccgcc ggacggatgg attcttatgt cgatcaactg caagcgcagg gcggaagtat
    59041 gatcatgctg gcgaaaggca accgcagcca gcaggtgacg gatgcctgta aaaaacacgg
    59101 cggcttctac cttggcagta tcggtggtcc ggccgctgta ttggcgcagg gaagtattaa
    59161 gagcctggaa tgtgttgaat atccggaact gggaatggaa gccatctgga aaattgaagt
    59221 ggaagatttc ccggcgttta tccttgtgga tgataaagga aatgacttct tccagcagat
    59281 acaactcaca cagtgcactc gctgtgtgaa ataaacagag ccgcccttcg gggcggtttt
    59341 tttacatggc acgaaagacc aaacatttgt tatcaaatag taaataataa gtgagctaaa
    59401 agttgcttaa cgaaagcaaa acagaaagaa aaaattaaac aggtgaggag caggccatga
    59461 atacagtacg cagcgaaaaa gattcgatgg gggcgattga tgtcccggca gataagctgt
    59521 ggggcgcaca aactcaacgc tcgctggagc atttccgcat ttcgacggag aaaatgccca
    59581 cctcactgat tcatgcgctg gcgctaacca agcgcgcagc ggcaaaagtt aatgaagatt
    59641 taggcttgtt gtctgaagag aaagcgagcg ccattcggca ggcggcggat gaagtactgg
    59701 caggacagca tgacgacgaa ttcccgctgg ctatctggca gaccggctcc ggcacgcaaa
    59761 gtaacatgaa catgaacgaa gtgctggcta accgggccag tgaattactc ggcggcgtgc
    59821 gcgggatgga acgtaaagtt caccctaacg acgacgtgaa caaaagccaa agttccaacg
    59881 atgtctttcc gacggcgatg cacgttgcgg cgctgctggc gctgcgcaag caactcattc
    59941 cgcagcttaa aaccctgaca cagacactga gtgaaaaatc gcgtgcattt gccgatatcg
    60001 tcaaaatcgg tcgaacccac ttgcaggacg ccacgccgct aacactaggg caggagattt
    60061 ccggctgggt agcgatgctc gagcataatc tcaaacatat cgaatacagc ctgcctcacg
    60121 tagcggaact ggctcttggc ggtacagcgg tgggtactgg actaaatacc catccggagt
    60181 atgcgcgtcg cgtagcagat gaactggcag tcattacctg tgcaccgttt gttaccgcgc
    60241 cgaacaaatt tgaagcgctt gcgacctgtg atgccctggt tcaggcgcac ggcgcgttga
    60301 aagggttggc tgcgtcactg atgaaaatcg ccaatgatgt ccgctggctg gcctctggcc
    60361 cgcgctgcgg aattggtgaa atctcaatcc cggaaaatga gccgggcagc tcaatcatgc
    60421 cggggaaagt gaacccaacc cagtgtgaag cattaaccat gctctgctgt caggtaatgg
    60481 ggaacgacgt ggcgatcaac atgggtggcg cttccggtaa ctttgaactg aacgtcttcc
    60541 gtccgatggt gatccataat ttcctgcaat cggtgcgctt gctggcagat ggcatggaaa
    60601 gttttaacaa acactgcgca gtgggtattg aaccgaatcg tgagcgaatc aatcaattac
    60661 tcaatgaatc gctgatgctg gtgactgcgc ttaacaccca cattggttat gacaaagccg
    60721 cggagatcgc caaaaaagcg cataaagaag ggctgacctt aaaagctgcg gcccttgcgc
    60781 tggggtatct tagcgaagcc gagtttgaca gctgggtacg gccagaacag atggtcggca
    60841 gtatgaaagc cgggcgttaa tctgcaacat acaggtgcag ccgtggaatg atcaaacgaa
    60901 gcggctgcgc ctgtggttta taacggtgct gcacattgtc ggcatcgtaa tttaacaact
    60961 caccaacgtc tggcacgccc gcaccattat cccgattaat cagtgcaatc agcggtgtag
    61021 ggcaggcgtg ttgcacttgt ttttgatctc ctttgtacca gacgcgggca atcggctgca
    61081 ccttcaccgg acgtttgatt tttaacttcg cgttctgtgg cagggcagcg atatcctgat
    61141 actctcgctc cagttttctt tgccactcct cgcgcgtcca cggtgcgaca ctgcgtggtg
    61201 atttcaggct tttttccagc tgtgccagga cttcatcacg atgcaaattc ttaatgatat
    61261 gtttattagc ccaaccaaag cgtaaagtgg cggggtcgtg cagaacggtg agcgtgcggt
    61321 aagcattaag ggtgatcagc cccggcaaat gacgatgcac ccattcaaaa cgtgccgcgg
    61381 tggggagttc tgattcaacc gtgacgatat gctcgaacgt ggtcttgagt ttattgatgt
    61441 gctgaatatg actgaccaac gctgcttgcg aaaggttatc gacctggtaa cacaacacgc
    61501 caggcagacg gacagcggcc ttgctgctgc gattttcgga ctgttgttga ataaataaat
    61561 ggcggaaatg acgcaacgcc agcgactgcg cgtcgttgcc gagatgttgt tttacctcaa
    61621 tacgattaag cggattatgc tcatcctctt tttttacctc cggcaaagag aacacgcggg
    61681 caaccaatag cttgtgttgc tcaagatgag cggcaaatgc agccagctct tgttccatct
    61741 ggcgaaaggt agtgttgagt cggtctacga gatcgtaacg cgccataata ttaaccactt
    61801 tagttacaac atacttattt tatagcacag tggtggtgac ttgaccatcg tgcgcgtagt
    61861 gtcaggcaga ggtaaattgc gggatgttat gccataacgg ccagctaaac gagaagcgag
    61921 caccacccag ttcgctggtg tcacaattaa ccgtaccgcc cattgccagt gctatagagt
    61981 ggacaattgc cagccccagc ccgcagccgc cggttgagcg atcccggctg ggatcgaggc
    62041 gaacaaaagg ttcaaagata tgttcgcggt tttctggggc aatccctggg ccatcatcct
    62101 caacaattaa tgtcgctcta ttccccgaca gtagcaggct ggtttcaacc gttgaatggc
    62161 agtaccgcag ggcgttattg agcaaattat ccagcacgcg ctccattaag cgcatatcca
    62221 acgccgcata atggccttgc gcgagcgttt taatccgtac cgttttatcg ggcgttactg
    62281 cctgaatgtc tgccagatgc gttgacagcc acaacggcag gtctggttcg ctaagatgaa
    62341 gctcgttttg tgggcgatcg agtcgggcat aagtcagcag ctcttcaatt aaagcttcaa
    62401 gttgactgat atcacgattc aacgcctggg attcggcggc gctcaggtta tcgctcatct
    62461 ccagtcgata acgcaggcgc actaacggtg ttcgcagttc gtgagcgata ccgtcaataa
    62521 gctgtttttt gctggcaatc aaggcgttga tattgtccgc catctggtta aatgcgatac
    62581 caagtcgttc aaagctcgaa ccctcatcaa agtggatacg ttcattgaga tgcccatcgc
    62641 caaatcgttg cgccgctgct tccagtttta acatatcctg ccagtgcgga cgcatccaga
    62701 taaacaccgg aaaggcgagg gaaatagcaa taaaagtgat cagggcgata tccagcaatc
    62761 gcatctgatg gagataataa agataaggaa caggaccaac tgccagcacg tagtggctgc
    62821 gcgggatacg ctgcaaaaac gtgtactgat cgtccagggc gacaatttcg ccgccacgca
    62881 gtcggtgcat ggaaatatca tcaagatggt atttactcag tggctcgaca cgcagatcga
    62941 aagagagatt taaatccatc tctttcagcg tcttacccca gtcgtgtggg gggatctctc
    63001 gcaattcgct gcgcatcaga tacagcgaac tgttcatcaa atcatccagc gactgtttgc
    63061 ccgcgcgttc ggcggtaaat ttgtacacca gcccaaccag cagagacatc acaaggaagc
    63121 agacaaacaa taacaggtaa aactggataa acagtttttt catcgcttat tcccatgcat
    63181 gaggcgcaaa aagatagcct ttgttacgca cagttttaat gcgataaggt tctgcggcgt
    63241 tatcgagcag tttttttctt aaccgcgaaa tagccacgtc cacgctacga tccagtccgt
    63301 cataactgac gccgcgtaaa tttttcagca atgcatcgcg gtccatgatt tgcccggcat
    63361 gggtagctaa ttcccacaat aattcgaaat cagctgtcga gagcgagatt tcagtgttag
    63421 ccagggtgac tacgcggttg atgggatcga tggtcaacgt gccgaaatgc agggctttgt
    63481 agggagtcag agacgtttcc tgaagacctt tggtcagtgt ggcttgctca ttctgacgca
    63541 aatgcaaacg taaacgcgct agcaaaacag cagggggcgt cgttttgaga atatagtcgc
    63601 aggcacccat ttccagtgcc aggatgtggt tcatatcgct atcgagagag gttagaagaa
    63661 caatcggtcc agaccacttt gcgcgtaaat cacgacaaat ggtcatgccg tccttgcctg
    63721 gtagcatgat gtcgagtaac accaaatccg gactttctcg caaaatggtt tcttcggcct
    63781 ggtcgccgcg cggttctacg gtaacctgca tatcatgttt tgccaggtac gcggcaatca
    63841 gtgaaccgac ttccgcatca tcttccacaa atacgatagt gttcataaca ttcacggtag
    63901 atataaaaac gtcacaatac accgcgctgt ttttacttac tatcaatcgt ttctaaacaa
    63961 gttttcatca ggtattctac tgatgacttg ttatggaagt gttaaggtaa aaagatgggg
    64021 ctggtaatca aagccgcgct cggtgcgctg gtagtgctgt tgattggtgt tttagcaaaa
    64081 acgaaaaatt attatatcgc cgggctgatt ccacttttcc cgacctttgc gcttatcgcg
    64141 cattatattg ttgccagcga acgcggcatt gaagccttac gcgcaaccat catttttagt
    64201 atgtggtcga ttattcccta ttttgtttac ctggtgtcgc tgtggtattt caccggaatg
    64261 atgcgcttgc ccgccgcttt tgttggttct gttgcgtgtt gggggataag cgcatgggtg
    64321 ttgattatat gctggataaa gctgcattaa cgcagatgac gaccgccatc aagtgggaaa
    64381 ctgcgtccgg tgacaaagca gctggtaagt aagtaatcga ccaggtcgat cacttctttc
    64441 tcgccaggcg cggttttcat cagtgattta ttcagcgcct gttgtcgata ttcggcatca
    64501 tcatgttcat taaacaggat cagcgatggc gcaatagaat tcactttcac ttccggtgcc
    64561 agcttgcggg caaacgagcg ggtcatatta tccagtgccg ctttgcttgc agcatacgca
    64621 acatgtttgt cgctaccgcg ctccaccaca taatcggtaa agtgaatgat atcgctggcg
    64681 gcgtgtccgt gcccacgcag taatctttcc agcgcatggt tgagcaggta tggggtatta
    64741 acgtggatct gcatcatgca agccagtacg tcggccagtg gtgtacctgg tttttccgcc
    64801 atccacgcac tggcgttatg caaaatagca cgcagaccat gggtgctttt tagtacttca
    64861 tcggcaaacg ccatcacacc gtcgttggtc gaaaaatcag cctgaataca ctgcgcacct
    64921 gccttaatca gtccatcaat ggctggatag tgcgtccgat agctgacaat caccggttgc
    64981 ttttgattaa tgaaatgcca ggcgagggcg aggccgatgc gacgacctcc gccagtaatt
    65041 aatattggca agggctgggc tttacccatc gttatctcct ttgctatcca acgatgggat
    65101 gaacgttatc ccaccagcat ccaggtcgcc ggaactgagg caattaacag catgccaatc
    65161 agcaccattt cctgacggtt cagtacatta tcatgtgtat gcgttttgcg cgcatacagg
    65221 aaaaccagca gccccggcgc atacagaaca acggataaca gcaagtgcat tggaccagag
    65281 gcgtacaata accataagcc ataaatgcag gcaccgacac ctaccgcttt atgcagtgga
    65341 cgggttgcga ttttcagcaa gaacgcgccg accaggaaat agggcaccag aatcatttct
    65401 gaggcgatgg tcagcaacgt gttgtaatcg gaaccggtca gccagatgag taccagacaa
    65461 atttgcacgc agatgttggt caaccacagc gaggcggatg gcgcagcttg tgcgttctgg
    65521 cgcgcaaaaa tgcgtgggaa tgctttatga gtggcagcca ggaacggaac ttctgccgcc
    65581 atgatggtcc agcttaagta cgcgccgcaa acggaaacga tcaaaccggc agcgatgatg
    65641 atttcgcccc atggtcccat catttccacc atcagaccgg ccattgacgg gttacgaatt
    65701 tcagccagtt caggacgtgc caccacgccc agcgaaagca gcgttaccag caagtaaacg
    65761 cccagagcgg agagaaccgc cagcagtgtc gctttgccaa catcacgttt attacgcgca
    65821 cgcgcagaaa caaccacggc accttccaca ccaatgaaaa cccacagggt gatcagcatg
    65881 gtgtttttca cctgttccca cacgggtacg ccaagtgcaa gtccggtgaa gtcgagcttg
    65941 aaggtatcca gtttgaacat catcatcgcc agcacaacaa acagacccag cggcaacaat
    66001 tttgccagtg tcgccaccag gttaatgctg gcagcggttt gcaccccgcg caggatcaaa
    66061 aagtgaacaa tccacaataa agccgatgca ccgactatcg attgccaggt attaccatcg
    66121 ccgaacaggc gcaattccgg cgtgtccgta aaaaagctta acgcggaaaa aacgatcacc
    66181 agataggaga cgttggcgat gactgcgcac agccagtatc cccatgcgga acaaaagccg
    66241 attagctcgc caaacccttc gcgggcatag gtaaagatac cgccgtcaag ttcgggacga
    66301 atgcgcgtga ggatcagcat ggcaaaggcc agcaataaaa tgccagcgcc agtaataccc
    66361 cagccgatga gcagtgctgc cgggctggca actgccgcca tattttgcgg cagactgaaa
    66421 acacccgcgc ccagcattga gcttaatacc agcgcggtga gtgcgctcag tcccagtttc
    66481 ttttccatcg atatccagtg attaaaacgt gataagaaga ataaatatta gggcaaaacg
    66541 cataaaaatg gagtttgcca ctgaagcgcg ggattttacg aagagatgtg tctgcatgca
    66601 atggtcgcgg ggaaatttgt gactaaaaat gcgaaaggcg gcatcaagcc gccttatagg
    66661 agtaactgaa tagttattta tacagatctg cgctgacggt caggttatta ccacgttcct
    66721 gccactggcg ggtgatgtgg taatacttag cacctttctt cgcggcacgt ttcgcaacct
    66781 gataggagac ttcggtcatg ttgccgtagt tgccagagaa tttgatgctg tcgaacggga
    66841 ccatcatcgc tgcggtcgct ttgttcagtt cttcgacttt agtgccatcc gggagcgtga
    66901 cggtgtaacg cccgcctttt gatgactggg tttcaaagaa acgaccgact tcagaacttg
    66961 gtgatgcggt agtcgcaaca cctgggatct caactttctt cgcggcttcg ccaccggcag
    67021 ccagagctgc acgtcctgct tcggaatctg ccgggatcac atccgggctc tggacgatac
    67081 gtttcttagc atctttttta tagatgaatg cagtaatacg ctggttgccg ccctggttgg
    67141 catcgatttg acgaacgatg tagaaagagt aggcaccttt cgcttttgcc gctttggtga
    67201 tggcatcatt gacttctggc tggctacgat agaagccctg gacggtgacc gtgtcaaacg
    67261 gttcaatcag aacagcctga tctttcggca gttcgacaac accgttaatt acgcgattac
    67321 ttgtttcttc tgctttttca gcatcggctt tatagaggtc agcgaccaca cgccagttac
    67381 cgctgttacc aaaatcagaa gtgtcgacaa cataaaaaga ggcggcacct tctttatctg
    67441 cgcgacgaga aacggctttc accgcttcgc caatagcatt aaaacgaccg gtaaccacta
    67501 cacggtcaaa aggtttaacc gctgccgctt gctccggtgt cagttctgtt gctgcgttaa
    67561 cggagaatgc cgtagcagaa agcagtgccg acgccaggag ggtgttctta agcttcataa
    67621 aaataatcct tcgccttgcg caaaccaggt actggtattg ttattaacga gaaacgtggc
    67681 tgattattgc atttaaacgg tgtaactgtc tgcgtcattt ttcatatcac attctttaag
    67741 ccaattttaa tcctgctcaa atgaccgtct atgcttaaaa aacagccgta tcagcatcat
    67801 tactactgaa gcaactgaat tatataagtt aatttaatgt taagtagtga ttcgtgccgg
    67861 ggcgatgtct cgttttaccc gaccgtcgaa gacaattatc agtctttatc cggcgttcta
    67921 aggtgtttat cccactatca cggctgaatc gttaatattt tgcgagttca cgccgaaata
    67981 ctgatttttg gcgctagatc acaggcataa ttttcagtac gttatagggc gtttgttact
    68041 aatttatttt aacggagtaa catttagctc gtacatgagc ggcttgtgtg gctcctgaca
    68101 caggcaaacc atcatcaata aaaccgatgg aagggaatat catgcgaatt ggcataccaa
    68161 gagaacggtt aaccaatgaa acccgtgttg cagcaacgcc aaaaacagtg gaacagctgc
    68221 tgaaactggg ttttaccgtc gcggtagaga gcggcgcggg tcaactggca agttttgacg
    68281 ataaagcgtt tgtgcaagcg ggcgctgaaa ttgtagaagg gaatagcgtc tggcagtcag
    68341 agatcattct gaaggtcaat gcgccgttag atgatgaaat tgcgttactg aatcctggga
    68401 caacgctggt gagttttatc tggcctgcgc agaatccgga attaatgcaa aaacttgcgg
    68461 aacgtaacgt gaccgtgatg gcgatggatt ctgtgccgcg tatctcacgc gcacaatcgc
    68521 tggacgcact aagctcgatg gcgaacatcg ccggttatcg cgccattgtt gaagcggcac
    68581 atgaatttgg gcgcttcttt accgggcaaa ttactgcggc cgggaaagtg ccaccggcaa
    68641 aagtgatggt gattggtgcg ggtgttgcag gtctggccgc cattggcgca gcaaacagtc
    68701 tcggcgcgat tgtgcgtgca ttcgacaccc gcccggaagt gaaagaacaa gttcaaagta
    68761 tgggcgcgga attcctcgag ctggatttta aagaggaagc gggcagcggc gatggctatg
    68821 ccaaagtgat gtcggacgcg ttcatcaaag cggaaatgga actctttgcc gcccaggcaa
    68881 aagaggtcga tatcattgtc accaccgcgc ttattccagg caaaacagcg ccgaagctaa
    68941 ttacccgtga aatggttgac tccatgaagg cgggcagtgt gattgtcgac ctggcagccc
    69001 aaaacggtgg caactgtgaa tacaccgtgc cgggtgaaat cttcactacg gaaaatggtg
    69061 tcaaagtgat tggttatacc gatcttccgg gccgtctgcc gacgcaatcc tcacagcttt
    69121 acggtactaa cctcgttaat ctgctgaaac tgttgtgcaa agagaaagac ggcaatatca
    69181 ctgttgattt tgatgatgtg gtgattcgcg gcgtgaccgt gatccgtgcg ggcgaaatta
    69241 cctggccggc accgccgatt caggtatcag ctcagccgca ggcggcacaa aaagcggcac
    69301 cggaagtgaa aactgaggaa aaatgtgcct gctcaccgtg gcgtaaatac gcattgatgg
    69361 cgctggcaat catccttttc ggttggctgg caagcgttgc gccgaaagag tttcttgggc
    69421 acttcaccgt tttcgcgctg gcctgcgttg tcggttatta cgtggtgtgg aatgtatcgc
    69481 atgcgctgca tacaccgttg atgtcggtca ccaacgcgat ttcagggatt attgttgtcg
    69541 gagcactgtt gcagattggc cagggcggct gggttagctt ccttagtttt atcgcggtgc
    69601 ttatagccag cattaatatt ttcggtggct tcaccgtgac tcagcgcatg ctgaaaatgt
    69661 tccgcaaaaa ttaaggggta acatatgtct ggaggattag ttacagctgc atacattgtt
    69721 gccgcgatcc tgtttatctt cagtctggcc ggtctttcga aacatgaaac gtctcgccag
    69781 ggtaacaact tcggtatcgc cgggatggcg attgcgttaa tcgcaaccat ttttggaccg
    69841 gatacgggta atgttggctg gatcttgctg gcgatggtca ttggtggggc aattggtatc
    69901 cgtctggcga agaaagttga aatgaccgaa atgccagaac tggtggcgat cctgcatagc
    69961 ttcgtgggtc tggcggcagt gctggttggc tttaacagtt atctgcatca tgacgcggga
    70021 atggcaccga ttctggtcaa tattcacctg acggaagtgt tcctcggtat cttcatcggg
    70081 gcggtaacgt tcacgggttc ggtggtggcg ttcggcaaac tgtgtggcaa gatttcgtct
    70141 aaaccgttga tgctgccaaa ccgtcacaaa atgaacctgg cggctctggt cgtttccttc
    70201 ctgctgctga ttgtatttgt tcgcacggac agcgtcggcc tgcaagtgct ggcattgctg
    70261 ataatgaccg ccattgcgct ggtattcggc tggcatttag tcgcctccat cggtggtgca
    70321 gatatgccag tggtggtgtc gatgctgaac tcgtactccg gctgggcggc tgcggctgcg
    70381 ggctttatgc tcagcaacga cctgctgatt gtgaccggtg cgctggtcgg ttcttcgggg
    70441 gctatccttt cttacattat gtgtaaggcg atgaaccgtt cctttatcag cgttattgcg
    70501 ggtggtttcg gcaccgacgg ctcttctact ggcgatgatc aggaagtggg tgagcaccgc
    70561 gaaatcaccg cagaagagac agcggaactg ctgaaaaact cccattcagt gatcattact
    70621 ccggggtacg gcatggcagt cgcgcaggcg caatatcctg tcgctgaaat taccgagaaa
    70681 ctccgcgctc gtggtatcaa cgtgcgtttc ggtatccacc cggttgcggg gcgtttgcct
    70741 ggacatatga acgtattgct ggctgaagca aaagtaccgt atgacatcgt gctggaaatg
    70801 gacgagatca acgatgactt tgctgatacc gataccgtac tggtgattgg tgctaacgat
    70861 acggttaacc cggcggcgca ggatgatccg aagagtccga ttgctggtat gcctgtgctg
    70921 gaagtgtgga aagcgcagaa cgtgattgtc tttaaacgtt cgatgaacac tggctatgct
    70981 ggtgtgcaaa acccgctgtt cttcaaggaa aacacccaca tgctgtttgg tgacgccaaa
    71041 gccagcgtgg atgcaatcct gaaagctctg taaccctgac ggcctctgcg gaggccgtca
    71101 ctctttattg agatcgctta acagaacggc gatgctttga cctcccgctg tttgttcaag
    71161 cgcaattttg acaataattg ttaacggcac ggaaagcagc atacccaccg gtcctaacaa
    71221 ccatccccaa aaaatcaacg acaaaaatac caccaatgtg gaaagcccca gcccacgtcc
    71281 catgatgcgc ggctccagaa tattgccgaa gaccagatta atcagcagat atcccgccag
    71341 caccagcaac gcttcgtaga agccattaaa caccagtacc tgagcgatag gggggattgc
    71401 cgcgagaact gaaccaatat tagggatgta attaaacgca aaggccatca atccccagac
    71461 aaaagcgaag cgaacatcga gtgcggcgag catcgcccag gcgaccaggc cggtgatgat
    71521 gctgatggct gttttcagca ccagataatg agaaacactg tcaattgcac gttgaatcgc
    71581 cgccatccct tcaaccggac gcgccatcat ttgctgaaat tttccgggca attgtggcac
    71641 ttcgagcagc ataaacagca ccgtcagcag caataaaaat attgatgaca tggcattaga
    71701 taactgcgtc aataagttgg tgagcaacgt catcgccgcg ttcggatcaa tgtaatgcgc
    71761 cagctggtca actgagacgt caatccctac gcgttgcaac aacggctcaa gagcttgcag
    71821 cggcgtcata atagagttgc gatattgcgg taacgtccgc gtcaactcgt tgagcgcgga
    71881 acccagataa gctaatagca acaccatcgc catcacgatg atggtcatca aaatcgacac
    71941 cgccagtaca cgcggcactc gccagcggac catgtgttgc accagcgggt taagaataac
    72001 agcaataaat aatgcgagaa taaacggcac gatgatctcg gcggcaaaac ggataccgca
    72061 gagaataatg accagcattc ccaacataat gacgattttt aggccattga gcgtgatgat
    72121 cggctttgcc atgcttgtcc ccgttttttc ttcctttgct aataataatg tctttttcgc
    72181 tccgtcatca taacgaaaat caaaccgctc aggtaaagaa gtgaaaatat tttgagttaa
    72241 ttcttaagct atgatacaaa tcaggcgtgt tcaactaccg aggacaatta tcatccgcga
    72301 tgacgagaag caacactgcg gataattgta atattatgga caatttgttc aggacgttat
    72361 tttctacatt tacccatctt cgtacctcat ccactatctt gctcgtaggt gagcagcact
    72421 ggcgcaacgc gctttagtcc tttcttctgc ctaagcttgc cctatgcgcg gggcttgacc
    72481 actttacttt ggtttcgctg aattaagcga aaattaaaat aattctcttg caggagaagg
    72541 acaatgtata tttattggat tttattaggt ctggctattg ctacagaaat taccggtacg
    72601 ctgtccatga aatgggcgag cgtcagtgag ggaaatggcg gctttatttt aatgctggtg
    72661 atgatttctc tgtcgtatat atttctctct ttcgccgtta aaaaaatcgc cttaggcgta
    72721 gcttatgcgc tgtgggaagg tatcggtatt ttatttatta ccttgtttag cgttatgtta
    72781 ttcgacgaaa gtttatcgct gatgaaaatt gccgggttaa ccaccctggt cgccgggatt
    72841 gtgttgataa aatcaggtac ccgtaaagcg cgtaaacctg aactggaggt gaaccatggc
    72901 gcagtttgaa tgggttcacg ccgcctggct ggcattggca atcgtgctgg aaatcgttgc
    72961 taacgtcttt ttgaaatttt ctgacggctt tcgtcgcaaa atatttggct tgctctccct
    73021 ggcggcggtg ctggctgcct ttagtgcgct ttctcaggcc gtaaaaggga tcgacttgtc
    73081 tgtcgcttat gcattgtggg gcgggtttgg tattgccgcc acgttagccg caggttggat
    73141 cttgtttggt caacggttaa atcgtaaagg ctggattggc ctggtcttgc tgttggctgg
    73201 aatgatcatg gtgaaacttg cctgatgaac acgctgcccg cactgtcggg cagcgtttga
    73261 acattatttt tgcgacagtt gatccagctt gtcgcgaaaa ccggtaacag aaatggcccg
    73321 gttatcggcg cgccagcgat ctttcgcggc aggagccgaa ctttgcaccc caattaattg
    73381 ccagccgtca tcggtatgca acatcagagg cgaaccgctg tcacccggca aggtatcgca
    73441 ctgatgtgac atcaccgacg tttgcgccca gccagtcact tcacagtttt gatgactgta
    73501 taacgtatcg agatgatctt caggatatcc cgcctgagtc actttacgac cagccgattt
    73561 taatgcggca gtaagcgcgg ctttatctcc ctcaaataac ggcaacggtg taatgccaga
    73621 agggggatta cgcagcacaa tcaagccgaa gtcccatggt gcagctgcgg gaggaacaat
    73681 ccaaccatcc ccatctgctt ttaaccgctt tcccagtgtc ggatcaacgc ggccttctat
    73741 gtcgtggatg tcatagcgcc aaagaccttt atttgacaca aaacgcagcg ctaccgcctt
    73801 atccgcttta cccttaggcg gcgtcaataa acagtgtcct gccgttaatg ccagattggg
    73861 tgcaatcagc gtcgccgtac ataaattgcc gctggccgtt tccagttgcc caaccgcatc
    73921 ccacggtgat tgggtcgtgt cattcactgg cacacgatca tcatgaccaa aaaacagggt
    73981 gctgacctca tcgtttgccg atttggcaac gtctggttta tctgcaaaca caaaagcaga
    74041 cgtcaaacta attgcaccca acactacagc aatggttgta cgcatatcac actctggtgg
    74101 gtaattatga ttattaaaag caaaccctca taaatactat agacgggacg ggatgaaagt
    74161 gggagtaaaa tcagataact acagtcagga aagataaaaa cgggcggcaa tcagtgcgca
    74221 taaaataagg atgataagaa tgaactcaaa gcgataacgg cccaccatac taccctccgc
    74281 taaaggcggt gctaaacgtt acccgcttag caccgtaatt ttcaggcgcg agggggcgcg
    74341 ccagcattac tgttgaaaac ttacgctgcg ggttgtgcag caggttttgc cggttgctga
    74401 tggctgtgtt tcccggcgtg tttcttcgct gcctgcgctt tttgttcagg ggctttctgt
    74461 tcagctttcg tatttttatg atgcttttta gccgcctgcg ctttctgggc aggtgctgct
    74521 ttatgctgtt ttttatgatg tgtagttttc gccggcgctg ctttggtggt cgtcgcagtc
    74581 ggagcaggtg tgttcgcagt ctctgcagca aaggcggcag aagacagacc catagcagcg
    74641 gcaacaacca gagctaatac ttttttcatt gtcataccct caatttgttt tttcatttaa
    74701 ccccactgcg gggccgttga aataactata tccctgggaa tggcagactt ccgtgagtgg
    74761 ttggtttcag cgtgtaacga tatgtacaaa cgctgaataa attacagcgt tgataataac
    74821 gtattgtgac ttaagggaaa tttagctacc aataatagta gtcttgatcg ggttaactat
    74881 tttactactt acaaagcggt agaataactg cgcatcagta ataaatgaca cacagcaaaa
    74941 tgaatccgtt tatttgggta cttataatcc tgatgacgct agacgcgctg cgggaattgg
    75001 ctggcgcttc gtctatttta ggatggctat taacgttggt ttgagcaagc aataagtccg
    75061 gacgggtagt taccgcagtc cgggcttatt tttcaggcgt gcagacgacg atgcaaacgc
    75121 gtcccgacca gcagagcaat gaccagcatc agagcaataa atgcgccgac gccgttccag
    75181 ccatagttat gccagaaaac accacccagc gtcccggcaa tactcgaccc cagatagtaa
    75241 ctgaacagat acagcgagga ggcctggcct ttagcgcgtt ttgcgcgggg gccaatccag
    75301 ctgctggcaa ctgagtgggc agcgaagaat cctgctgaga agagtaacat tccggcaaag
    75361 atcagccaca gcgagctgaa taaggtcatc agtaaaccaa acagcataac ccccgtcgaa
    75421 aacaacatca ccggaccgcg cccgtagcgg gtggtcatgg ttccggcttt gggtgagctc
    75481 catgtaccgg tcaaataagc cagcgataat aagccaacca cggcctgact gacatgccag
    75541 ggggaaagca tcaaccgata gccgatgtaa ttaaacagcg tgacgaacga ccccatcagc
    75601 aaaaagcctt ctgcgaacaa taacggtaat ccccggtcac gccagtgcag acgaaagttg
    75661 ataaataacg tcttagggcg cagcgaagtc gggcgaaaat ggcgtgattc cggaaggatt
    75721 ttccagaaca tcaacgccga ggccagcgcg aaacaaccga ttgccgccag agcaattcgc
    75781 cagttgaaaa agtccgtgaa gacaccacta attaagcgtc cgctcatgcc gccaattgag
    75841 ttgccgctga tatacaaccc catcgaaaag gccacgaaac tggggtggat ttcctcgcta
    75901 agataagtca tgccaacagc tgccacgcca cttaacgaaa gcccaatcaa ggcgcgcata
    75961 atcaaaatgc cgtgccagct ggtcatcatt gtcgaaagta acgtacaaat ggaggccaac
    76021 agtagcgccg tgaccatcac tggtttgcga ccaatggcat cggatagggg gccagtaaac
    76081 agcaaaccaa tagccaacat cgccgtggaa atggacagtg aaatactact gttcgcgggc
    76141 gttaagccaa actcctgcga aagcaccgga aggataggct gcacacaata gagaagtgca
    76201 aatgttgcca gtccggcaga gaacagcgcc agggtgacgc gcataaattg cggcgtaccg
    76261 cgtttaataa attgatttgg ctgagaaatg ctttgcttgt cagtgtcgct tgccggagcg
    76321 ccatcaacag ttgtagtacg gctcacttga aatccttgct aaatatgcct gtggatcagg
    76381 cttatacata ggttaggaaa atcgaattgt tctgtctaat atattaataa tctcaaataa
    76441 gatgttttaa atatgaatat tgaacttcgt catctgcgtt actttgttgc tattgcggaa
    76501 gagctgcatt tcgggcgcgc cgctgcccgc ctgaatattt cgcaaccacc gctaagtcag
    76561 cagattcagg cactggagca acaaattggt gcccgactgc tggcacgaac taatcgcagt
    76621 gtattgctga cggcagcagg aaaacagttt cttgcagata gtcggcaaat cctgtctatg
    76681 gtagatgacg ctgccgctcg cgctgaaaga ctgcatcagg gggaagcggg ggagttgcgc
    76741 attggtttta cttcgtccgc tccttttatt cgggcggtgt ccgatacgtt atcgctgttt
    76801 cgccgtgatt atcccgatgt ccatttacaa acccgcgaaa tgaacactcg tgagcaaatc
    76861 gctccgctca ttgaaggaac gctggatatg ggattgctgc gtaacacagc gttaccggag
    76921 acgcttgaac acgcagtcat cgtccatgaa ccgcttatgg cgatgatccc gcacgatcat
    76981 cccctggcaa ataacccgaa tgtaacgctg gctgaactgg cgaaagaacc cttcgtcttt
    77041 tttgatccgc acgtcgggac agggctgtat gaagatattc tcgggctgat gcgacgttac
    77101 catttgacgc ccgtcatcac tcaggaggtg ggcgaggcaa tgaccatcat cggtctggtt
    77161 tccgccggtc tgggtgtttc aattttgcct gcgtcattta aacgtgttca gctcaacgaa
    77221 atgcgctggg tgccgattgc agaagaggat gcggtttctg aaatgtggtt ggtctggccg
    77281 aaacatcatg aacaaagtcc agctgcgcga aactttcgta ttcatctgct gaatgctctc
    77341 aggtgaggga aatttcagcg aaaaagcccg aaaaatgtgc tgttaatcac atgcctaagt
    77401 aaaaatttga cgacacgtat tgaagtgctt caccatagcc tacagattat ttcggagcgc
    77461 gaaaatatag ggagtatgcg gtggttgctg aaaaccagcc tgggcacatt gatcaaataa
    77521 agcagaccaa cgcgggcgcg gtttatcgcc tgattgatca gcttggtcca gtctcgcgta
    77581 tcgatctttc ccgtctggcg cagctggctc ctgccagtat cactaaaatt gtccgtgaga
    77641 tgctcgaagc acacctggtg caagagctgg aaatcaaaga agcggggaac cgtggccgtc
    77701 cggcggtggg gctggtggtt gaaactgaag cctggcacta tctttctctg cgcattagtc
    77761 gcggggagat tttccttgct ctgcgcgatc tgagcagcaa actggtggtg gaagagtcgc
    77821 aggaactggc gttaaaagat gactcgccat tgctggatcg tatcatttcc catatcgatc
    77881 agttttttat tcgccaccag aaaaaacttg agcgtctaac ttcgattgcc ataaccttgc
    77941 tgggaattat tgatacggaa aatggtattg tacatcgcat gccgttctac gaggatgtaa
    78001 aagagatgcc gctcggcgag gcgctggagc agcataccgg cgtaccggtt tatattcagc
    78061 atgatatcag cgcatggacg atggcagagg ccttgtttgg tgcctcacgc ggggcgcgcg
    78121 atgtgattca ggtggttatc gatcacaacg tgggggcggg cgtcattacc gatggtcatc
    78181 tgctacacgc aggcagcagt agtctcgtgg aaataggcca cacacaggtc gacccgtatg
    78241 ggaaacgctg ttattgcggg aatcacggct gcctcgaaac catcgccagt gtggacagta
    78301 ttcttgagct ggcacagctg cgtcttaatc aatccatgag ctcgatgtta catggacaac
    78361 cgttaaccgt ggactcattg tgtcaggcgg cattgcgcgg cgatctgctg gcaaaagaca
    78421 tcattaccgg ggtgggcgcg catgtagggc gcattcttgc catcatggtg aatttattta
    78481 acccacaaaa aatactgatt ggctcaccgt taagtaaagc ggcagatatc ctcttcccgg
    78541 tcatctcaga cagcatccgt cagcaggccc ttcctgcgta tagtcagcac atcagcgttg
    78601 agagtactca gttttctaac cagggcacga tggcaggggc tgcactggta aaagacgcga
    78661 tgtataacgg ttctttgttg attcgtctgt tgcagggtta acatttttta actgttctac
    78721 caaaatttgc gctatctcaa tttgggccag gaaagcataa cttagacttt caaggttaat
    78781 tattttcctg gtttatattt gtgaagcata acggtggagt tagtgatgct gaagcgtttc
    78841 tttattaccg gtacagacac ttctgtaggg aaaacggtgg tttcccgcgc attgctacaa
    78901 gcgttagcct cccagggaaa aacggttgcg ggatataaac ccgtagcgaa ggggagcaaa
    78961 gagacacccg aagggctgcg taataaagat gccctggtgt tgcagagtgt ttcaaccatc
    79021 gaactgcctt atgaagcagt taatcctatc gcgttaagcg aagaagaaag tagcgtggcg
    79081 cacagttgcc caatcaatta caccctcatt tcaaacggcc tggcaaacct gaccgaaaaa
    79141 gtcgatcatg tcgtggtaga agggactggc ggctggcgca gtctgatgaa tgatttgcgt
    79201 ccactctctg aatgggtagt gcaggaacaa ctgccggtgt tgatggttgt cggtattcag
    79261 gaaggttgca ttaaccatgc actgctaaca gctcaggcga tcgccaacga cgggctgccg
    79321 ctcattggct gggtggctaa ccgaatcaac ccaggactgg cgcattatgc ggaaatcatt
    79381 gatgtgctgg gtaaaaaact tccggcaccg ctcattggtg aactgcctta tctgccgcgc
    79441 gccgaacagc gtgaactggg gcaatacatc cgcttagcta tgctgcgcag tgtgctggcg
    79501 gtagatagag tcacggtgta acgtccgcga aattaccgac gcaattacgc aggcaatcaa
    79561 taaaccgggg agtagctgat actccccggt catttcacat atcatcaacg tcgacataat
    79621 cggcgcgtgc gtggtcgccg ccaacagtgt cgccatcccg gtcaatccga gtaaaagtgt
    79681 aatttcttcg ccatcaggga accataatcc caggctacga ccatacaaca tgccaatggc
    79741 aagaccgata aatagcgtcg gtgtaaagac cccaccgggt gcgccggaac cgctactcgc
    79801 cagcacggca cacagtttac aaaggaagat cccggcaatg atcattaaca gtggtggggc
    79861 ggttaaaaag gattgtacgg tgctatagcc gttgccccac actgcaggtg tgaacaggga
    79921 aagcagaccc acgatcaacc cgcctagtgc cagttgccag ggcggcgcaa gtttgagact
    79981 cacaaatcca cgatgacagg cgttcattaa cgttaacaac agtggtccgc acagacctgc
    80041 cagcacacct gtactgataa tcagcgcata gtcacgagcc tgaaccgtca ctgagagttg
    80101 tacgttgtag agtaacgcgt cgctatgatt aatcagattg ctaaccagca atgccacgac
    80161 ggcggaaata atcaccgggc cgagagaggc caacatcata gtgccaaaca gcacttcggc
    80221 tataaataaa ctgccagcaa gcggggcacg ataggccgca gccattcccg ccgcggcccc
    80281 acaggcgatc cataatttcc actcctggcg tggcgtaaaa cgttgggcaa aacaggaggc
    80341 ggcaagggca gctaaaagaa tcatcgcacc ttcgcgacca attgcactgc cgctggttac
    80401 taccagcagc gaggcaagcg atttaaccag gcttgctgcg taatcgaact gtccatcggt
    80461 ttgcaacgct tccatgtaat cggtcggcgc atgagggcgt tgttgggtaa atttctgcca
    80521 gcccatcagc aacaaacccg ccgccagtcc gccgagcgcc ggagttagca accgtcgcca
    80581 gggggaaagg tttgtcgctg cattgaccag actgccggag tcattattga ggaacaacca
    80641 ctccagtagc agcatcgcat gacgaaaccc ggcaacggca aaggccgcga gaataccgac
    80701 gactgttgcg ataagcagac ggcggaacat agtgcgcaga tccggataga tgtgtagatg
    80761 atgcatgagc agaaatgggc aatgaaacac gggcgactat tttgcgacaa aagaggccag
    80821 ccgcaaaagc agtgcgtgct ttaagagata aaaaaacgtg acactgtaac ctattattgc
    80881 cggattgcgc gtaatcgtca ccatccggca atattacggt gatcctatcg aaacagcggt
    80941 ttaaccgcga caggaatcaa cagttgtgat tgccattgcg ccagcgttaa gcgagccagt
    81001 tcacccagtg cacggtagaa agggtgttct gctttttcga taaaaacatc aagaaaacgt
    81061 gttgaccacg gaaaaaggtg ccatgccagc aattcttcgc attctgtctg gcgaccattc
    81121 tccgccaacc acgcagccat caacagcagt gaaccaaaat gatcttccgg ttcgttttgc
    81181 ttcatttcaa actgaatgcc tttctcgcgc atccactgac gaagtgccaa tgttgaatcg
    81241 ccaaacagca cagattcgcg atccagccag accgaccccc acggcggaga cggcagtgcc
    81301 cacgggccga caaacaaacg ctgccaggcc tgggcgtgag tctcttcaca ctgtgtctga
    81361 aaagccgtca ccagcggcgc taatgacgct tctggtagag gccactgagt ttcccagccg
    81421 tcactggtga gtaccgcaac cagaggtgcg gcttccgcac tctctggagc gtaataaaac
    81481 agcgcgccca acacgcgcgc cgcgacagaa aaattatctt gctgtgaaaa atgggtcatt
    81541 cctaacatcc tgaaatgtgc gggttgcccc gcaccaattt gttaacctgc aatagccatt
    81601 cctaccgtca tgtgcaggcc ataaaagagc acacgcccga tcatctcacc gccaagaatg
    81661 aggattaacc ccagaataag tccggcaacg tgaggttcac ggcgacgaat gagcggacaa
    81721 acccagcatc ctagacctgc gcataacaat accacacgcc agacctgtaa ggaggcgtaa
    81781 tctggtacca gtgcgctggc ttgttgtacg gatgagtgaa tacacgccag agacaaacct
    81841 tgcagcacaa tcaccccggc acaagcgatc agcgccagaa cgctaatgat ggcaaatggt
    81901 gtggtattaa atgtaacgcg agctgcccgc agaattgcgg cagcaagtat tggaccgctc
    81961 aacaataccg tcaggaagaa agccagcgta gtgtaaccgt tatgccaggt tggcacggtg
    82021 tcgatttgat acacgcaggt catcatccag acgaaaatga cgccgagcgc catgctgaca
    82081 agtaaccaga gtttccccaa cgcttgcggc attttaccga tgaccgccac cagccaccat
    82141 agtccgccaa cggcaaaaaa tatggaaccg gcagcaatct cgttactcag accagaagct
    82201 ccgattcgat tcagcgagtt aaacgctcgc agtggcgatc ccaggtgcat gacagaggca
    82261 ataaacccga tgcccatcaa cagccagagg aaaaacatgc cacggacaat acgctgacgg
    82321 tctgcgtcat ttttggcggc gaaccagcca attccgctaa caatcagcgc gcctactacg
    82381 cattgaccca atacggtaaa gataaccagt ggccattcat gccatccatt tcccatttta
    82441 cacttcctcc ggattagcca gataaccagt ggtatcgccg gtcgggcggc tgttggcgtt
    82501 aggtttgata acgatattgg gttttgtgaa atgcgcgcgc ggcagtggcg cgacggcagc
    82561 cagagtgccg tgcttctggc gcagctcttc aattggaccg aactcaagtg cacgcagcgg
    82621 gcaggattcg acgcatatgg gttgtttccc ctcggcgacg cgcgaataac aaccatcgca
    82681 cttcgtcatg tgcccttttt cagcattgta ctgtggtgcg ccgtacggac aagccatgtg
    82741 gcagtagcgg cagccgatac aaacatcttc atcaaccacc acaaagccat cttcacgctt
    82801 atgcattgcg ccgctggggc agacttttgt acatgctggg tcatcgcaat ggttacagga
    82861 gatggagaga taataagcga acacgttctg gtgccagacg ccattatctt cctgccagtc
    82921 gccgccagca tattcataaa tacggcggaa actgacttcc gggccaagat ctttaaaatc
    82981 tttgcacgcc agttcgcagg ttttacagcc agtacagcgg ctggaatcga taaaaaatcc
    83041 atattgtgtg gtcatgggct actctttaaa ccttttcgat ctggacaaga ttgctgtgcg
    83101 acgggtttcc ctttgccagc ggtgaagggc ggtgagaggt cagaatattg atactgccgc
    83161 cgtgatcgac ccggtcacca aacatatccg ctttaagcca cgcaccttgc ccgatggcgg
    83221 taacgccagg cagaatacgc ggagtcactt ttgcggcaat cagcatttct ccattattgt
    83281 taaatacccg cacggtatcg ccatgacgga taccgcgagc ctgagcatca atggggttga
    83341 tccacacctc ttgtgggcag gcctgctgta acacatcaat attgccgtag ctggagtggg
    83401 tacgcgcctt gtagtggaag cccgttaact gcagtggata ggttttccgc aggggatcgt
    83461 cccagccatc aaaacctggc gtataagctg gaaggggatg aataatttca tcttttttca
    83521 attcccaggt atccgcaatc ttcgccagtc gttcagaata aatttcgatt ttccccgaag
    83581 gtgttttcaa cgggtttgcc tggggatctt cacggaatgc gcggaaagcg acgtagtgtt
    83641 cttccgggca ttttttctta aagatcccgg tcgttttcat ctcctcgtag tcgggcatct
    83701 cagggttacg ttccttcgtt ttcgcatgga gatatttgat ccattcatgc tgactgcgac
    83761 cttcagtaaa ggtttgataa acgtctggtc ctaagcgttt ggcgacttca ctcagcatcc
    83821 agtagatggg tttgcgttca aattttgcag aggttgcggg ttgggcgagg atcacatagc
    83881 ccatattccc tgcagattca tgagagataa ggtcttcttg ctctgttggc atcaggtcgg
    83941 gcaacaggat atcgcaatac ttagccgagg ccgtcatgaa gtggtcaatg ccaacaatca
    84001 tctcgcactt gctgtcatcc tgaagcacct catgggtgtg attgatgtcg ccatgttgat
    84061 tgatcaatgt gttactggcg tagcaccata aaaacttgat ggggacatcc agtttttctt
    84121 ttccacgaac accatcacgg gtcgcggtca tttccttacc atggtcgatg gcatctgtcc
    84181 atgtaaagac ggaaatctgc gttttaacag ggttctcaag catcgggaac cattctaccc
    84241 ccagatccca gctaccttcg cgtacacctg agttgccgcc gtttatgccg acgttaccgg
    84301 tgagaacgga aagcatggca atagcgcggg acgtttgctc gccgttggaa tgtcgttgtg
    84361 gcccccaacc ctgacaaata taagcaggtt ttgctgaacc gatctctcgt gccaactgga
    84421 taattttttc tgccgggatg ctggtgattt ttgctgccca ttccggcgtt ttagctatgc
    84481 cgtcaggccc ttcgcccaga atataggctt tataatgcgc gttacgtggt gcgttggcgg
    84541 gcagcgtttt ttcatcgtaa ccaacacaat atttgtcgag aaatggctga tcgaccatgt
    84601 tttcagtaat cagtacccag gcaatcgcac aggccagtgc gccatcggtg cctgggcgaa
    84661 tgggcagcca ttcatcttca cgcccggcag ctgtgtcgtt ataacgtgga tcgatgacga
    84721 tcatgcgtgc gtttgaacgt tcgcgggctt gctcgacgta gtaagtgaca ccaccgccgc
    84781 tcatccgcgt ttctgccggg ttatttccga acataacgac cagtttcgta ttggcgatat
    84841 catccgggct gttgccatca ttggcaccga acatataact cattgcggca ctgatctgtg
    84901 cggtactgta gctgccatag cgactgagaa aaccaccgca agagttcatc agacggtacg
    84961 ggacgtttga gttggtgatg tttccgccat ctacgcctgt tccgtacagg acatgtacag
    85021 cctcattgcc gtaatctttt aggatccgcc gaagattatc actgatggta tccagggctt
    85081 cgtcccaact tatccgttca aatttacctt caccgcgctt gccgacgcgc ttcatgggat
    85141 atttcaacct atcaggatga ttcatccgtc ggcggataga gcgcccgcgt aaacacgcgc
    85201 gaacctgatg attaccgtag acgtcgtcac ctgtcgtatc agactccacc cagtacacgg
    85261 tgtcatcttt cacatgcaaa cgtaacagac agcggctccc gcagttaacg gtgcaggaac
    85321 tccagaccgc tttctcttct accggagcct ctgccgcccg gaccatttgg gaaaatggca
    85381 gagtgaaagc actgcttgcc agcgcaagac tgccaagtgc ggaggttttc atcagacttc
    85441 tacggctgat ttcagccttc atgagcgcct ctgtggtatg gattttcatc attactcact
    85501 tattgctttt caaacaaaat gtcatgccag aatttatggt tgtagtgggt tatatttttt
    85561 cgatctcgac cagattagtg tgctgcgggt ttcccttcgc cagtggtgaa gggcgcagag
    85621 tggttagcgt attcacacag ccgccatggt cgattttatc gccagacata ttggcctcgt
    85681 gccaggctcc ctggcccata gcgctaaccc cagggagaat gcgtggtgtc actttggctg
    85741 gtagccgaac ttcgccacga tggttaaaca cccgcaccat atcgccgttg gcaatcccac
    85801 gtttctgcgc atctataggg ttgatccaca cctcctgacg gcaggcagcc ttcaggacat
    85861 caatgttgcc gtaggtcgag tgagtacggg atttgtaatg gaaaccaaac agttgcagtg
    85921 ggaaggtact acgttcaggg gagtcccagc cttcaaaggt tgaggcataa actggcaatg
    85981 ggcttatcac ttcatctttt tccagttccc aggtacgggc aatttccgcc agcctgctgg
    86041 aataaatttc aatcttaccg gaaggcgttt taagtggatt tgcctcgggg tcgtcacgaa
    86101 atgctttgta ggcgacaaaa tgaccattgg gatctttacg cttatagata cccatttttt
    86161 tcagttcgtc gtaagacggt aacgctggat ctttggcaag cattttggcg tacagatgtt
    86221 gtaaccattg ttcctgcgtg cgaccttctg tgaacttttg atagacgtca ggtccaagac
    86281 gtttcgcgac ttcactcagg atccagtaaa tcggtttacg ttcgaatttt tcgctggtga
    86341 ctggctggag gaaaatgaga tatcccatgt taccggcgta gtcgttagga ataatatctt
    86401 cctgctcaac ggtcatcagg tctggcagca gaatgtcggc atattttgcc gatgaggtca
    86461 taaagttttc gatgaccaca atcatttcgc atttcgattc gtcttgcaga atttcatgcg
    86521 ttttgttgat gtcagaatgc tgattaacca gggtatttcc cgcgtagttc cagatgaact
    86581 taatgggcac atccagttta tctttgccgc ggacgccgtc gcggattgcc gtcatttgcg
    86641 gaccatgatc gatagcatcc gtccagctga agcaggagat tgacgttttg accggattat
    86701 ccagcaccgg caggcgttct atggtaatgg tataggtcga ttctcgcgcg ccactatttc
    86761 cgccgctgat gccgacattg cccgtcaaaa taggcaacat agcaatagcg cgtgcagtca
    86821 gctcgccgtt tgcctggcgt tgcggccccc agccctggca gatataagcg ggttttgctg
    86881 tgccaatttc acgcgccagt ttgatgatac ggtccaccgg gataccggta atttgcgaag
    86941 cccactgcgg cgttttcgct gtgttatcat caccttcacc aagaatatag gctttatagt
    87001 gaccattttt tggtgcatct gcgggtaagg ttttttcgtc atagccgacg cagtatttat
    87061 cgagaaaagg ttgatcaacg agattttcgt taatcaatac ccaggcaata cccgcaacca
    87121 gcgcggcatc ggtgcccggg cgaataggaa gccattcatc ttcacgacca gcagtcgtat
    87181 cggtatatcg cggatcgata acaatcattt tggcgttcga tttctcgcgc gctttttcaa
    87241 gaagataagt gatgccaccg ccgctcatgc gggtttctgc cgggttgtta ccaaacatca
    87301 cgaccagctt gctgttttca atatccgtgg tgctgttgcc atcattactg ccgtaggtgt
    87361 agggcatggc acaggaaatt tgcgcggtgc tgtaggagcc atactgattg agtgaaccgc
    87421 cgtagcagtt catcaggcgt ttgaccgccg aggctgatgg cgaagagcgg gtcatattgc
    87481 cgccaacaat ccccgaagag tactgaatat atacagcctc attgccatat tgttcgacgg
    87541 tttttttcag gctactggcg atagtatcca gggcttcatc ccagctaatc cgttcgaatt
    87601 tgccttcgcc gcgtttgccc acgcgtttca ttggataatt caagcgatcg ggatgattaa
    87661 tacgccgacg gatggagcga ccgcgcaaac aggcgcgtac ctgatggttg ccgtactcat
    87721 cgctgccggt attgtcagtt tccacccagg tcacttcatt atctttaaca tgtagacgaa
    87781 gtgcacagcg gctaccacag ttgacggaac aggcacccca gaccactttt tcgcgggcct
    87841 gttgtaccgc tgctgctgca ctgcgcaggg taaacggcaa agaaaaaccg cctgcagcca
    87901 gcgccagaga acctatcgcg gtagatttaa cgagtgttct gcggctgatg cccaccattc
    87961 gttcattttt ggacataact cactccctgt tctttatcgt tatataaaag tttatatatt
    88021 gaatatttag cgcgctaaca atagagggag tctacccatt ttgggttaag aattattaat
    88081 ccatatcaat agaagggtat gagtaataag gtgggattat gttgtatgtt caaatcgccg
    88141 gatgtgtcgt atccggcgtt cagtcgataa tgtgttactg cggttcggca ggcgcgccat
    88201 cctgggtaga ctgcgcggga gcagagacgt taccgctggt ggtgcgggtg tagagaattt
    88261 tatgcgtatc attagcacaa tgcccgacga cctgggaatc aggctgatca acctggtcat
    88321 tgggtacaat acttaacgtg aagctgcttt cgggtacgcc attattgata atgcgctgtg
    88381 atatatcgct ctgtatgcgc tcacaggatc ccggcgcggc gagtaccgcg ggtgaggcga
    88441 gggcgagcag aagcgcggca cagcaggttg agagtttcat cataagctcc ttacgcgaag
    88501 ataacttctt taagcatagc atttaacgtg taaagtactg tatttgctac tatgattgag
    88561 aatcatctct actctctggt gactgttgtg aaatacaaat tactaccatg cttactcgcg
    88621 atattcctca caggatgtga ccgcacagag gtaacacttt catttacccc tgagatggcc
    88681 agtttctcta atgaattcga ttttgatccg ctgcgtggtc cggtaaaaga tttcactcag
    88741 acattaatgg atgagcaagg tgaagtgacg aaacgtgttt ctgggacttt gtcggaagaa
    88801 ggctgttttg attcactcga attactggat ctggaaaata ataccgtggt cgctctggta
    88861 ctggacgcca attattaccg tgatgctgag acgctggaga aaagagtacg tttacaggga
    88921 aaatgccagc tagcagaatt accttctgcc ggggtgagtt gggaaaccga tgataatggc
    88981 ttcgtgatta aagccagcag caaacaaatg cagatggaat atcgctatga tgatcagggg
    89041 tatccgctgg gtaaaaccac gaaaagtaac gacaaaacat tatctgtcag cgccacgcca
    89101 tcaacggacc cgatcaaaaa attagattat acagcagtta ctttactgaa taatcaacga
    89161 gttggtaatg taaaacagag ctgtgaatat gacagtcacg ccaatccggt ggactgtcag
    89221 ctgatcattg ttgatgaagg agtaaacccc gccgtcgaac gggtttacac catcaaaaat
    89281 acgatcgatt attattaatg ctattgtgcg gtcggcttca ggagagtctg acccggtgtt
    89341 ttgtgctctg ccagatactg atgctggaat atacacatgc gaatggcatt acgatattga
    89401 ccattaataa agaactcgtg catcaattca ccttcaaccg aaaagccaag cttgcggtaa
    89461 atatgaatcg ctttttcatt ctctttatca acgatcagat acagcttata gagattgaga
    89521 acggtaaagc catagtccat tgctaatttt gcggcacggg ttgccagacc tttcccctga
    89581 tactccgggg agataattat ctgaaattct gcgcggcgat gaacatggtt aatttccacc
    89641 agctccacca gaccggcttt ttcgccgtca cattccacca caaagcgccg ttcgctctga
    89701 tcgtgaatat gcttatcata cagatcagag agttcaacaa aggcttcgta gggttcctca
    89761 aaccagtaac gcatcacact ggcgttattg tcgagttgat gtacatagcg taaatcttca
    89821 cgctccagcg ggcgtagctt aacactgtgg gcgcttggca taacgtgtcc ttacattcct
    89881 taaatcaata acaggttagg gggtaataac gcggccagtt cgacggtcca ggcagcgcaa
    89941 agtatttggc tcccagtagg cattgatgtt ggcgctttgc tcacatttat cgcggttatc
    90001 aaaagcggcg tcggctttat cccactcttt ttcagtgcgt ttattcactt tctggcgcag
    90061 attgcgcgtg tcattccatt gctctttttc catagcggcg tgctggcggc tttgtgcact
    90121 gtcgccagac tcaatcacca gtttgttagt ttcggcatga acagttgtgc tcaatgccag
    90181 tgcgcaaggt agcagaaaag cgagcaggcc gattcgtttg ctgagagtga ttttcataat
    90241 tcattccctg tatgaatgat taaaggtgat tctacaccat ccactgcgaa cgcaaaacgt
    90301 atcaggaggg tgtttatatt ggtgatatta tgtggcccta taactataca tgatgtcaat
    90361 aagtgacaaa gatgattaaa acaacgttac tattttttgc tactgcgctg tgtgaaatta
    90421 ttggatgctt tctgccctgg ttgtggttaa aacgaaacgc cagtatctgg ctgttgcttc
    90481 cggcggggat ttcactggcg ctgtttgtct ggttgttaac gttgcatcct gcggcgagtg
    90541 ggcgtgttta cgcggcttat ggtggcgttt atgtctgcac ggcgttgatg tggctgcgcg
    90601 ttgtggatgg cgtgaaactg actctttatg actggacggg tgcgttgatt gcgctttgcg
    90661 gcatgttgat cattgttgcg ggctgggggc gcacgtagga acataaatcc attttatcaa
    90721 taagatacga ggaagtgtca gctgacaaaa ggtattctat ttcatctttt gtcagccatt
    90781 cacagcgcaa atatacgcct ttttttgtga tcactccggc ttttttcgat tctcatactt
    90841 gtatggtagt agctcagttg cgtagatttc atgcatcacg acaagcgatg caaggaatcg
    90901 aacatgaaga tcgtaaaggc tgaagttttt gttacctgtc cggggcgtaa tttcgtcaca
    90961 ttaaaaatca ccactgagga cggtattacg ggccttgggg atgccactct caatggacgt
    91021 gagctttccg tggcctctta tttgcaggat cacctttgtc cgcagcttat tggtcgcgat
    91081 gcgcaccgta tcgaagatat ctggcagttt ttctataaag gtgcttactg gcgtcgcggt
    91141 ccggttacga tgtcggccat ttcagcggtt gatatggcgc tgtgggatat taaagccaaa
    91201 gcggccaaca tgccgcttta ccagttactc ggcggcgcgt ctcgtgaagg ggtgatggtt
    91261 tattgccata ccaccggtca cagtattgat gaagctctgg atgattatgc ccgtcatcag
    91321 gagctgggat tcaaagccat ccgcgtgcag tgcggaatcc ctggtatgaa aaccacctac
    91381 ggcatgtcga aaggtaaagg tctggcttat gaacccgcaa ccaaaggaca gtggccggaa
    91441 gagcagctgt ggtcgacgga gaaatacctc gatttcatgc cgaaattgtt tgacgcggta
    91501 cgtaacaagt ttggttttaa tgaacatttg ctgcatgaca tgcaccatcg cttaacgcct
    91561 attgaagcgg cgcgctttgg taaaagcatt gaagattatc gcatgttctg gatggaagac
    91621 ccgacacctg cggaaaacca ggaatgcttc cgtctcattc gccaacatac cgtcacaccc
    91681 atcgcagtgg gtgaagtctt caacagcatc tgggactgca aacaactgat tgaagagcaa
    91741 ctcatcgatt atatccgcac cacgctgacc catgcaggcg gaattaccgg tatgcgccgg
    91801 attgccgatt ttgcttcgct gtatcaggta cgtactggct cacacggtcc ttccgatttg
    91861 tcaccagtct gcatggctgc ggcgctgcac tttgatctgt gggtccccaa tttcggtgtc
    91921 caggaataca tgggttattc cgaacaaatg ctcgaagtct tcccgcacaa ctggactttc
    91981 gataacggct atatgcatcc gggagacaaa ccgggtcttg gtatcgaatt cgatgaaaag
    92041 ctggcggcga aatatcccta tgaacctgct tatctaccag tcgcacgtct ggaagatggc
    92101 acgctgtgga actggtaagg agtaagataa tgaaaagcat attaattgaa aaaccgaatc
    92161 aactggcgat tgtcgaacgt gaaataccca ccccgtcagc gggtgaagtg cgagtaaaag
    92221 tgaaacttgc cggaatttgt ggttcagata gccatattta tcgtgggcat aatccttttg
    92281 cgaaatatcc gcgcgtcatt ggtcatgaat tctttggcgt cattgatgca gtgggtgaag
    92341 gcgtggaaag cgccagagtc ggtgaacgtg ttgctgtcga tccggtggtc agctgtgggc
    92401 attgctatcc gtgctctata ggtaaaccga acgtttgtac gacactggct gtattaggtg
    92461 tgcacgctga cggtggtttc agtgaatatg ccgtggttcc ggcaaaaaat gcgtggaaaa
    92521 ttcctgaagc agtggccgat caatatgcgg taatgatcga accttttacc attgcggcta
    92581 acgtaaccgg acatggtcaa ccgactgaaa atgataccgt tctggtttat ggtgccggtc
    92641 caatcggcct gacgatcgtt caggtattaa aaggcgtcta taacgttaaa aatgtgattg
    92701 ttgccgatcg cattgatgaa cgactggaaa aagcgaaaga gagcggggct gactgggcga
    92761 ttaataacag ccagacaccg cttggcgaga ttttcactga aaaaggcatc aagccgacat
    92821 taattatcga tgcggcttgt catccttcta tcctgaaaga ggccgtaacg ctggcttctc
    92881 cagcggcacg tattgtattg atgggcttct ccagtgaacc gtctgaagtg attcagcaag
    92941 gaattaccgg aaaagaactc tctatttttt cttcacgctt aaatgcaaat aaattcccgg
    93001 tcgttatcga ctggttaagt aaagggttaa ttaaaccaga aaaattaatt acccatacgt
    93061 ttgatttcca gcatgttgct gatgccatta gtttatttga acaggatcaa aaacattgct
    93121 gcaaagtctt actcactttt tctgaataat accaataacg gcgagtaagt agtacgcatc
    93181 ttacctcttt tttagagata accattatga caatagaaaa acacgaaaga agcactaagg
    93241 atttggtgaa agcagcagta tcgggatggc tgggcactgc gcttgaattt atggatttca
    93301 agagtcattc aatctaacta attgataaat aaaaaattaa cttttcatcg cttcgttatg
    93361 gggcatggtt ggggcaaact cgcttaactg tgtatttaac agcgttacct gcgcattatt
    93421 attttctgac atccatttcc cgtacacctg aaacaccatt tgcgcatctg catggcccat
    93481 ctggtttgca atgaatgccg ggttagctcc tgctgttaac gaccagcagg cataagtgtg
    93541 tctcgactga tacgatttgc gatggcggat tccggcacgc tttaccgctg cgtcccacat
    93601 ctgccttatt gagtcaacgg taaaatggtc accataattt tttactttcg ctgacacttc
    93661 aggttgaaaa acaaaggtac atttttgttt ctctgttctg ccgaattctc tgaggtgaac
    93721 atcgatgata tgctctttgc tcagtctcgt tagtgccatc tgactccgga gagcgtcgat
    93781 tgcgggatta ataaggtgaa tcacacgatt ggttcccgcc tgtgtttttg gtactgtaaa
    93841 acggtctttt gccagatttc ttctgattat cattgttcca tttttcagat ctatatcttc
    93901 ccatccaagc gcacacagtt caccagggcg aatcccagta taaacggaaa cacaccataa
    93961 atttttggct tgctgatttc tgcacgcgtc aataaggcgg ataaactcct cccgcgaaag
    94021 tggatccggg atggttctcg actcctttaa tggtgagatc cccttaaatg gattgtttgc
    94081 cagataaccg ttatcaacac caaactggaa cacggcgtaa agatttgtca tgtaattatt
    94141 tacggtaaca gccgatctcc ctggttgtgt aacaatatag ttacttttgg ggatctggta
    94201 tccagtcagt aactctttac gtaccgccag caatttttct ttattaatcg atgatgcaag
    94261 atttttttca ccgattatgc tcagaatatt tttgatgacg gcacggtacg tgttgagtga
    94321 cgtttttgcg acttcagttt ctttcagtgc cagaaatttt tcagccagtt cttttatggt
    94381 taaatcttgt cgggcctcac caaatttttc cagattgtgt gaggcgggaa actgttttgc
    94441 atagtcgaaa gcgccagttt ttattgcgta acaaacagag gcgcgtagct cacctgcaat
    94501 gcgcctgttt tttgctgtgt caggaacccc aagattttcc cggactctta cacctttata
    94561 aacaaaccag atacgtaatt tccctccatg gttttccacg cctgtcggat atttcatttc
    94621 agcttctctc attggttcgt gttgctttta gtcaagcaag atgacgtctt ggtcttgcag
    94681 atgcctggcg ctcaatccag cgatcaattt cttccaggtt gtaaaagcat ggactgttat
    94741 cccatggcat accgtcatga gcgacatgct tatattccct tccttccata aacgattttt
    94801 ccctggcttt ttttagcgtt ccctttttga ttcctttaag tgcaattaac tgctcttcgg
    94861 atacccattt gccaggagag acgatcatga ttacttcgct catcgattct ttatctctta
    94921 catcagatga acgccggttg cagaatacca attgcaaccg acgacggttg aacattaaaa
    94981 atcagcctga ctcgggatca gttttttcca gatagctgaa acgtattttg cctgataacg
    95041 ggcgtcatca agtgcattat ggcgctcacc ttcgaatggg atagcagttc tgacatcgaa
    95101 gtctatggct ttccccagct caacgattgt gcgtacatcg cgatcgttgt agtaacgcca
    95161 cgggcagggg
//
LOCUS       DAZFYC010000006        90881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFYC010000006 DAZFYC010000000
VERSION     DAZFYC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.006"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..590
                     /gene="yraH"
                     /locus_tag="ACHKW5_000523"
     CDS             6..590
                     /gene="yraH"
                     /locus_tag="ACHKW5_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7509970.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            670..1363
                     /gene="yraI"
                     /locus_tag="ACHKW5_000524"
                     /pseudo
     CDS             670..1363
                     /gene="yraI"
                     /locus_tag="ACHKW5_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            1393..3909
                     /gene="yraJ"
                     /locus_tag="ACHKW5_000525"
     CDS             1393..3909
                     /gene="yraJ"
                     /locus_tag="ACHKW5_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7509971.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALYL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3920..5011
                     /gene="yraK"
                     /locus_tag="ACHKW5_000526"
     CDS             3920..5011
                     /gene="yraK"
                     /locus_tag="ACHKW5_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7509972.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5054..5914)
                     /gene="rsmI"
                     /locus_tag="ACHKW5_000527"
     CDS             complement(5054..5914)
                     /gene="rsmI"
                     /locus_tag="ACHKW5_000527"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO7509973.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5978..7966
                     /gene="lpoA"
                     /locus_tag="ACHKW5_000528"
     CDS             5978..7966
                     /gene="lpoA"
                     /locus_tag="ACHKW5_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO7509974.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTTQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTPLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFR
                     LEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQV
                     QGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7924..8319
                     /gene="yraN"
                     /locus_tag="ACHKW5_000529"
     CDS             7924..8319
                     /gene="yraN"
                     /locus_tag="ACHKW5_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO7509975.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8339..8929
                     /gene="diaA"
                     /locus_tag="ACHKW5_000530"
     CDS             8339..8929
                     /gene="diaA"
                     /locus_tag="ACHKW5_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO7509976.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8939..9514
                     /gene="dolP"
                     /locus_tag="ACHKW5_000531"
     CDS             8939..9514
                     /gene="dolP"
                     /locus_tag="ACHKW5_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7509977.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9628..10668)
                     /gene="yraQ"
                     /locus_tag="ACHKW5_000532"
     CDS             complement(9628..10668)
                     /gene="yraQ"
                     /locus_tag="ACHKW5_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7509978.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10741..11376)
                     /gene="yraR"
                     /locus_tag="ACHKW5_000533"
     CDS             complement(10741..11376)
                     /gene="yraR"
                     /locus_tag="ACHKW5_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO7509979.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11504..12022
                     /gene="yhbO"
                     /locus_tag="ACHKW5_000534"
     CDS             11504..12022
                     /gene="yhbO"
                     /locus_tag="ACHKW5_000534"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO7509980.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12002..12445)
                     /gene="yhbP"
                     /locus_tag="ACHKW5_000535"
     CDS             complement(12002..12445)
                     /gene="yhbP"
                     /locus_tag="ACHKW5_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO7509981.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12496..12798
                     /gene="yhbQ"
                     /locus_tag="ACHKW5_000536"
     CDS             12496..12798
                     /gene="yhbQ"
                     /locus_tag="ACHKW5_000536"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO7509982.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(12785..13288)
                     /gene="yhbS"
                     /locus_tag="ACHKW5_000537"
     CDS             complement(12785..13288)
                     /gene="yhbS"
                     /locus_tag="ACHKW5_000537"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7509983.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13282..13806)
                     /gene="ubiT"
                     /locus_tag="ACHKW5_000538"
     CDS             complement(13282..13806)
                     /gene="ubiT"
                     /locus_tag="ACHKW5_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO7509984.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14015..15010
                     /gene="ubiU"
                     /locus_tag="ACHKW5_000539"
     CDS             14015..15010
                     /gene="ubiU"
                     /locus_tag="ACHKW5_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO7509985.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15019..15897
                     /gene="ubiV"
                     /locus_tag="ACHKW5_000540"
     CDS             15019..15897
                     /gene="ubiV"
                     /locus_tag="ACHKW5_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO7509986.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAICSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16103..17110
                     /gene="yhbW"
                     /locus_tag="ACHKW5_000541"
     CDS             16103..17110
                     /gene="yhbW"
                     /locus_tag="ACHKW5_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO7509987.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLNLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17228..18472)
                     /gene="mtr"
                     /locus_tag="ACHKW5_000542"
     CDS             complement(17228..18472)
                     /gene="mtr"
                     /locus_tag="ACHKW5_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO7509988.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18626..20515)
                     /gene="deaD"
                     /locus_tag="ACHKW5_000543"
     CDS             complement(18626..20515)
                     /gene="deaD"
                     /locus_tag="ACHKW5_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO7509989.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20508..20588)
                     /gene="yrbN"
                     /locus_tag="ACHKW5_000544"
     CDS             complement(20508..20588)
                     /gene="yrbN"
                     /locus_tag="ACHKW5_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO7509990.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20695..21579)
                     /gene="nlpI"
                     /locus_tag="ACHKW5_000545"
     CDS             complement(20695..21579)
                     /gene="nlpI"
                     /locus_tag="ACHKW5_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO7509991.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21688..23823)
                     /gene="pnp"
                     /locus_tag="ACHKW5_000546"
     CDS             complement(21688..23823)
                     /gene="pnp"
                     /locus_tag="ACHKW5_000546"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO7509992.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24070..24339)
                     /gene="rpsO"
                     /locus_tag="ACHKW5_000547"
     CDS             complement(24070..24339)
                     /gene="rpsO"
                     /locus_tag="ACHKW5_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO7509993.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24488..25432)
                     /gene="truB"
                     /locus_tag="ACHKW5_000548"
     CDS             complement(24488..25432)
                     /gene="truB"
                     /locus_tag="ACHKW5_000548"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO7509994.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25432..25833)
                     /gene="rbfA"
                     /locus_tag="ACHKW5_000549"
     CDS             complement(25432..25833)
                     /gene="rbfA"
                     /locus_tag="ACHKW5_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO7509995.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25997..28669)
                     /gene="infB"
                     /locus_tag="ACHKW5_000550"
     CDS             complement(25997..28669)
                     /gene="infB"
                     /locus_tag="ACHKW5_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO7509996.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28694..30181)
                     /gene="nusA"
                     /locus_tag="ACHKW5_000551"
     CDS             complement(28694..30181)
                     /gene="nusA"
                     /locus_tag="ACHKW5_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO7509997.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30209..30661)
                     /gene="rimP"
                     /locus_tag="ACHKW5_000552"
     CDS             complement(30209..30661)
                     /gene="rimP"
                     /locus_tag="ACHKW5_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO7509998.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30868..30944)
                     /locus_tag="ACHKW5_000553"
     tRNA            complement(30868..30944)
                     /locus_tag="ACHKW5_000553"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30908..30910),aa:Met,seq:cat)
     gene            31292..32635
                     /gene="argG"
                     /locus_tag="ACHKW5_000554"
     CDS             31292..32635
                     /gene="argG"
                     /locus_tag="ACHKW5_000554"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO7509999.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32643..34268)
                     /gene="yhbX"
                     /locus_tag="ACHKW5_000555"
     CDS             complement(32643..34268)
                     /gene="yhbX"
                     /locus_tag="ACHKW5_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7510000.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVERTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34725..34811)
                     /locus_tag="ACHKW5_000556"
     tRNA            complement(34725..34811)
                     /locus_tag="ACHKW5_000556"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34775..34777),aa:Leu,seq:gag)
     gene            complement(34826..35158)
                     /gene="secG"
                     /locus_tag="ACHKW5_000557"
     CDS             complement(34826..35158)
                     /gene="secG"
                     /locus_tag="ACHKW5_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO7510001.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35386..36723)
                     /gene="glmM"
                     /locus_tag="ACHKW5_000558"
     CDS             complement(35386..36723)
                     /gene="glmM"
                     /locus_tag="ACHKW5_000558"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO7510002.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36716..37564)
                     /gene="folP"
                     /locus_tag="ACHKW5_000559"
     CDS             complement(36716..37564)
                     /gene="folP"
                     /locus_tag="ACHKW5_000559"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO7510003.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVVPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37654..39588)
                     /gene="ftsH"
                     /locus_tag="ACHKW5_000560"
     CDS             complement(37654..39588)
                     /gene="ftsH"
                     /locus_tag="ACHKW5_000560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO7510004.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39688..40317)
                     /gene="rlmE"
                     /locus_tag="ACHKW5_000561"
     CDS             complement(39688..40317)
                     /gene="rlmE"
                     /locus_tag="ACHKW5_000561"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO7510005.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40443..40736
                     /gene="yhbY"
                     /locus_tag="ACHKW5_000562"
     CDS             40443..40736
                     /gene="yhbY"
                     /locus_tag="ACHKW5_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO7510006.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40892..41368)
                     /gene="greA"
                     /locus_tag="ACHKW5_000563"
     CDS             complement(40892..41368)
                     /gene="greA"
                     /locus_tag="ACHKW5_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO7510007.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41616..43049
                     /gene="dacB"
                     /locus_tag="ACHKW5_000564"
     CDS             41616..43049
                     /gene="dacB"
                     /locus_tag="ACHKW5_000564"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7510008.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43089..44261)
                     /gene="cgtA"
                     /locus_tag="ACHKW5_000565"
     CDS             complement(43089..44261)
                     /gene="cgtA"
                     /locus_tag="ACHKW5_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO7510009.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44277..45242)
                     /gene="yhbE"
                     /locus_tag="ACHKW5_000566"
     CDS             complement(44277..45242)
                     /gene="yhbE"
                     /locus_tag="ACHKW5_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7510010.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45369..45626)
                     /gene="rpmA"
                     /locus_tag="ACHKW5_000567"
     CDS             complement(45369..45626)
                     /gene="rpmA"
                     /locus_tag="ACHKW5_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO7510011.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45647..45958)
                     /gene="rplU"
                     /locus_tag="ACHKW5_000568"
     CDS             complement(45647..45958)
                     /gene="rplU"
                     /locus_tag="ACHKW5_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO7510012.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46217..47188
                     /gene="ispB"
                     /locus_tag="ACHKW5_000569"
     CDS             46217..47188
                     /gene="ispB"
                     /locus_tag="ACHKW5_000569"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO7510013.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVQEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47416..47694
                     /gene="sfsB"
                     /locus_tag="ACHKW5_000570"
     CDS             47416..47694
                     /gene="sfsB"
                     /locus_tag="ACHKW5_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO7510014.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47742..49001)
                     /gene="murA"
                     /locus_tag="ACHKW5_000571"
     CDS             complement(47742..49001)
                     /gene="murA"
                     /locus_tag="ACHKW5_000571"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO7510015.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49056..49310)
                     /gene="ibaG"
                     /locus_tag="ACHKW5_000572"
     CDS             complement(49056..49310)
                     /gene="ibaG"
                     /locus_tag="ACHKW5_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO7510016.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49470..49763)
                     /gene="mlaB"
                     /locus_tag="ACHKW5_000573"
     CDS             complement(49470..49763)
                     /gene="mlaB"
                     /locus_tag="ACHKW5_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO7510017.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49763..50398)
                     /gene="mlaC"
                     /locus_tag="ACHKW5_000574"
     CDS             complement(49763..50398)
                     /gene="mlaC"
                     /locus_tag="ACHKW5_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO7510018.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50417..50968)
                     /gene="mlaD"
                     /locus_tag="ACHKW5_000575"
     CDS             complement(50417..50968)
                     /gene="mlaD"
                     /locus_tag="ACHKW5_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO7510019.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50973..51755)
                     /gene="mlaE"
                     /locus_tag="ACHKW5_000576"
     CDS             complement(50973..51755)
                     /gene="mlaE"
                     /locus_tag="ACHKW5_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO7510020.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51763..52572)
                     /gene="mlaF"
                     /locus_tag="ACHKW5_000577"
     CDS             complement(51763..52572)
                     /gene="mlaF"
                     /locus_tag="ACHKW5_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO7510021.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52782..53759
                     /gene="yrbG"
                     /locus_tag="ACHKW5_000578"
     CDS             52782..53759
                     /gene="yrbG"
                     /locus_tag="ACHKW5_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO7510022.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53773..54759
                     /gene="kdsD"
                     /locus_tag="ACHKW5_000579"
     CDS             53773..54759
                     /gene="kdsD"
                     /locus_tag="ACHKW5_000579"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO7510023.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELEQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54780..55346
                     /gene="kdsC"
                     /locus_tag="ACHKW5_000580"
     CDS             54780..55346
                     /gene="kdsC"
                     /locus_tag="ACHKW5_000580"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO7510024.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKTFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55343..55918
                     /gene="lptC"
                     /locus_tag="ACHKW5_000581"
     CDS             55343..55918
                     /gene="lptC"
                     /locus_tag="ACHKW5_000581"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO7510025.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55887..56444
                     /gene="lptA"
                     /locus_tag="ACHKW5_000582"
     CDS             55887..56444
                     /gene="lptA"
                     /locus_tag="ACHKW5_000582"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO7510026.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56451..57176
                     /gene="lptB"
                     /locus_tag="ACHKW5_000583"
     CDS             56451..57176
                     /gene="lptB"
                     /locus_tag="ACHKW5_000583"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO7510027.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57224..58657
                     /gene="rpoN"
                     /locus_tag="ACHKW5_000584"
     CDS             57224..58657
                     /gene="rpoN"
                     /locus_tag="ACHKW5_000584"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO7510028.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58680..58967
                     /gene="hpf"
                     /locus_tag="ACHKW5_000585"
     CDS             58680..58967
                     /gene="hpf"
                     /locus_tag="ACHKW5_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO7510029.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59085..59576
                     /gene="ptsN"
                     /locus_tag="ACHKW5_000586"
     CDS             59085..59576
                     /gene="ptsN"
                     /locus_tag="ACHKW5_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO7510030.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLEAPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59622..60476
                     /gene="rapZ"
                     /locus_tag="ACHKW5_000587"
     CDS             59622..60476
                     /gene="rapZ"
                     /locus_tag="ACHKW5_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO7510031.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60473..60745
                     /gene="npr"
                     /locus_tag="ACHKW5_000588"
     CDS             60473..60745
                     /gene="npr"
                     /locus_tag="ACHKW5_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO7510032.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60959..61591
                     /gene="yrbL"
                     /locus_tag="ACHKW5_000589"
     CDS             60959..61591
                     /gene="yrbL"
                     /locus_tag="ACHKW5_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO7510033.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61588..62316)
                     /gene="mtgA"
                     /locus_tag="ACHKW5_000590"
     CDS             complement(61588..62316)
                     /gene="mtgA"
                     /locus_tag="ACHKW5_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO7510034.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62313..62966)
                     /gene="elbB"
                     /locus_tag="ACHKW5_000591"
     CDS             complement(62313..62966)
                     /gene="elbB"
                     /locus_tag="ACHKW5_000591"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO7510035.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63196..65532)
                     /gene="arcB"
                     /locus_tag="ACHKW5_000592"
     CDS             complement(63196..65532)
                     /gene="arcB"
                     /locus_tag="ACHKW5_000592"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO7510036.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLCFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65628..66557)
                     /gene="yhcC"
                     /locus_tag="ACHKW5_000593"
     CDS             complement(65628..66557)
                     /gene="yhcC"
                     /locus_tag="ACHKW5_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO7510037.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67232..71692
                     /gene="gltB"
                     /locus_tag="ACHKW5_000594"
     CDS             67232..71692
                     /gene="gltB"
                     /locus_tag="ACHKW5_000594"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO7510038.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71705..73123
                     /gene="gltD"
                     /locus_tag="ACHKW5_000595"
     CDS             71705..73123
                     /gene="gltD"
                     /locus_tag="ACHKW5_000595"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO7510039.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEDNMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73307..74434
                     /gene="yhcG"
                     /locus_tag="ACHKW5_000596"
     CDS             73307..74434
                     /gene="yhcG"
                     /locus_tag="ACHKW5_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO7510040.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74494..74958)
                     /gene="nanQ"
                     /locus_tag="ACHKW5_000597"
     CDS             complement(74494..74958)
                     /gene="nanQ"
                     /locus_tag="ACHKW5_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO7510041.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74955..75830)
                     /gene="nanK"
                     /locus_tag="ACHKW5_000598"
     CDS             complement(74955..75830)
                     /gene="nanK"
                     /locus_tag="ACHKW5_000598"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO7510042.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQWLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75827..76516)
                     /gene="nanE"
                     /locus_tag="ACHKW5_000599"
     CDS             complement(75827..76516)
                     /gene="nanE"
                     /locus_tag="ACHKW5_000599"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO7510043.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76564..78054)
                     /gene="nanT"
                     /locus_tag="ACHKW5_000600"
     CDS             complement(76564..78054)
                     /gene="nanT"
                     /locus_tag="ACHKW5_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO7510044.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWGLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78163..79056)
                     /gene="nanA"
                     /locus_tag="ACHKW5_000601"
     CDS             complement(78163..79056)
                     /gene="nanA"
                     /locus_tag="ACHKW5_000601"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7510045.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79178..79969)
                     /gene="nanR"
                     /locus_tag="ACHKW5_000602"
     CDS             complement(79178..79969)
                     /gene="nanR"
                     /locus_tag="ACHKW5_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO7510046.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            80349..81716
                     /gene="dcuC"
                     /locus_tag="ACHKW5_000603"
     CDS             80349..81716
                     /gene="dcuC"
                     /locus_tag="ACHKW5_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7510047.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(81759..82256)
                     /gene="sspB"
                     /locus_tag="ACHKW5_000604"
     CDS             complement(81759..82256)
                     /gene="sspB"
                     /locus_tag="ACHKW5_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO7510048.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(82262..82900)
                     /gene="sspA"
                     /locus_tag="ACHKW5_000605"
     CDS             complement(82262..82900)
                     /gene="sspA"
                     /locus_tag="ACHKW5_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO7510049.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(83295..83687)
                     /gene="rpsI"
                     /locus_tag="ACHKW5_000606"
     CDS             complement(83295..83687)
                     /gene="rpsI"
                     /locus_tag="ACHKW5_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO7510050.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83703..84131)
                     /gene="rplM"
                     /locus_tag="ACHKW5_000607"
     CDS             complement(83703..84131)
                     /gene="rplM"
                     /locus_tag="ACHKW5_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO7510051.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(84350..85477)
                     /gene="zapE"
                     /locus_tag="ACHKW5_000608"
     CDS             complement(84350..85477)
                     /gene="zapE"
                     /locus_tag="ACHKW5_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO7510052.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85671..86069
                     /gene="zapG"
                     /locus_tag="ACHKW5_000609"
     CDS             85671..86069
                     /gene="zapG"
                     /locus_tag="ACHKW5_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO7510053.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            86223..87590
                     /gene="degQ"
                     /locus_tag="ACHKW5_000610"
     CDS             86223..87590
                     /gene="degQ"
                     /locus_tag="ACHKW5_000610"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO7510054.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87680..88747
                     /gene="degS"
                     /locus_tag="ACHKW5_000611"
     CDS             87680..88747
                     /gene="degS"
                     /locus_tag="ACHKW5_000611"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO7510055.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88809..89747)
                     /gene="mdh"
                     /locus_tag="ACHKW5_000612"
     CDS             complement(88809..89747)
                     /gene="mdh"
                     /locus_tag="ACHKW5_000612"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7510056.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            90182..90652
                     /gene="argR"
                     /locus_tag="ACHKW5_000613"
     CDS             90182..90652
                     /gene="argR"
                     /locus_tag="ACHKW5_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO7510057.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22414 a        24367 c        22530 g        21570 t
ORIGIN      
        1 tataaatgaa taaagttaca aaaacagcta ttgtgggctt acttgcgctt ttcgcaggta
       61 atgccgctgc aaccgatggt gaaatagttt ttgatggcga gattttaaaa tccgcttgtg
      121 aaatcaatga ctctgataag aaaattgaag ttgctcttgg tcactataat gccgaacagt
      181 ttcgttctgt tggcgatcgt agcccaaaaa ttccattcac tattccttta gttaactgcc
      241 cagtgactgg gtgggagcac gataacggca acgttgaagc gtctttccgt ttatggctgg
      301 aaacgcggga taatggcacc gtccctaact tccctaatct ggcaaaagtg ggctcttttg
      361 ctggcacagc ggcaacaggt gtgggtatcc gtattgacga tgcggaaagc ggaaacctta
      421 tgccactgaa tgctatgggg aatgataaca cggtttatca gatcccagcg gactccaatg
      481 gtattgtcaa tgttgacctc atcgcttact acgtatcaac cgttgaagca tcagaaatca
      541 ccccagggga agcagacgct gtcgttaacg taacgctgga ttaccgttaa tacgttacgg
      601 cgttatctga cctttcagat aacgcccttt tccttcctct ttctcgttgt atcaggttga
      661 aaaataacta tgtcaaaacg aacattcgcg gtgataataa ccttgttgtg tagcttctgt
      721 atcggccagg cgcttgcagg gggtatcgtt ttacagcgaa cgcgagtgat ctatgatgcc
      781 agccgcaaag aggctgcgtt acctgtcgca aacaaaggcg cagaaacgcc ttatttactg
      841 caatcatggg tagataatat agatggtaca agccgtgccc catttattat aaccccaccg
      901 ctatttcgtc ttgaagctgg cgatgactca tcactgcgaa ttattaaaac agctgataac
      961 ctgcctgaaa ataaagagtc gctgttctac attaatgttc gtgccattcc agcaaagaaa
     1021 aaatcagatg atgttaatgc taacgagttg acgctggtat ttaaaacacg gatcaaaatg
     1081 ttttatcgcc ccgcacacct gaagggacgg gtaaacgatg cgtggacatc actggaattt
     1141 aaacgtagtg accattcact caatatataa cccaactgaa tattacgtcg tatttgccgg
     1201 actggcagtc gataaaaccg atctcacaag caaaattgaa tatatcgcgc ccggagaaca
     1261 taaacagtta ccacttcctg catctggcgg aaagaacgtg aaatgggctg caatcaatga
     1321 ttatggcggc agttccggga cagaaactcg tccactgcaa taacaaatat aaaaaacaca
     1381 ggtcatcagg gaatgccaca acgacaccac cagggacata aacgcacacc gaaacagttg
     1441 gcgctcatca tcaaacgctg tttgccgatg gtgctcactg gcagcggcat gctttgcact
     1501 accgctaacg ccgaagagta ttatttcgac cccattatgc tggaaactac aaaaagtggt
     1561 atgcaaacaa ctgatctgtc acgtttttca aaaaaatacg cacaactacc aggaacttat
     1621 caggttgata tctggctgaa taaaaagaag gtttcacaga aaaaaattac atttaccgcc
     1681 aatgcagagc aacttctgca gccacagttt acggtagaac aactacgtga gctgggtatt
     1741 aaggtggatg aaatcccggc gctggctgaa aaagatgacg atagcgtgat caactcgctt
     1801 gaacaaatca ttcccggtac agctgctgaa tttgatttca atcatcagcg acttaatttg
     1861 agcattcccc aaattgcact gtaccgtgat gcaagaggtt acgtctcccc ttctcgttgg
     1921 gacgatggta taccaacgct gtttaccaac tactcgttta caggttctga taaccgttac
     1981 cgccagggca atcgtagcca acgacagtac ctgaatatgc aaaatggtgc taattttggc
     2041 ccctggcgat tacgtaacta ttctacgtgg acacgcaacg atcaggcgtc aagctggaac
     2101 actatcagta gttatttaca acgtgatatc aaggcgttga agtctcagtt gcttctggga
     2161 gaaagcgcca ccagcggcag tattttttcc agctacacct ttactggcgt gcaactcgct
     2221 tccgacgata atatgttgcc aaacagccag cgcggatttg ccccaacggt acgcggtatc
     2281 gcaaacagta gtgcaatcgt gactatcagg caaaatggtt atgtgatcta tcaaagcaac
     2341 gtgccagcgg gtgcctttga aattaacgat ctctacccct cttccaacag cggcgattta
     2401 gaagtcacga ttgaagaaag tgacggtacg caacgtcgct ttatccagcc ttattcttca
     2461 ttacccatga tgcagcgacc tgggcatcta aaatatagcg cgaccgctgg acgctatcgc
     2521 gctgatgcaa acagtgatag caaggaaccc gaatttgctg aagccacggc aatatatggt
     2581 ttgaataata cttttacgct gtatggcggc ctgctcggtt ctgaagatta ttatgcgctg
     2641 gggatcggta tcggcggcac acttggcgca ctgggcgcgt tgtcgatgga tatcaacaga
     2701 gctgacaccc aattcgataa ccagcactct tttcatggct atcaatggcg tacgcagtac
     2761 atcaaagata tcccggaaac caacaccaat atcgctgtca gctactatcg ctataccaac
     2821 gatggctatt ttagttttaa tgaagccaat acccgtaatt ggaactataa cagtcgccaa
     2881 aaaagtgaaa ttcaattcaa catcagccag acaatatttg atggggtaag tctgtatgcc
     2941 tccggttcgc agcaagacta ttggggcaat aacgagaaaa acaggaatat ctctgttggg
     3001 gtttccggtc agcaatgggg aattggttac agcctgaatt atcaatacag tcgctacacg
     3061 gatcaaaata atgaccgcgc actctatttg aatctcagta ttccgttaga acgctggtta
     3121 ccgcgtagcc gggtttccta tcagatgacc agccagaaag atcgcccaac ccaacatgaa
     3181 atgcgtcttg atggctcact gctggatgat ggtcgcctga gctatagcct ggaacaaagt
     3241 ctggatgacg ataacaacca taacagtagc ctgaacgcca gttaccgttc accttatggc
     3301 accttcagtg ccggatacag ctacggtaat gacagcagcc aatacaatta cggcgttacc
     3361 ggcggcgtgg ttatccatcc tcatggcgtg acgctctcgc aatatctggg caacgctttt
     3421 gcgcttatcg atgctaatgg ggcttctggc gtgaggatac aaaactatcc ggggattgct
     3481 actgatccct ttggctatgc agtggttcct tatctcacga cttaccagga aaaccgtctc
     3541 tcggtagata ctacgcagct gcccgataac gtcgatcttg agcaaacaac acagtttgtg
     3601 gtgcccaaca gaggtgcaat ggtagcggcg cgtttcaacg ccaatatcgg ttatcgcgta
     3661 cttgttacag tcagcgatcg caacggtaaa ccgttgccct ttggcgctct tgccagcaac
     3721 gatgagacgg ggcaacaaag tatcgtcgat gagggcggca tactatatct ctctgggata
     3781 tcgagtaaat cacaaagctg gactgtacgc tggggaaatc aggcagatca acaatgtcag
     3841 tttgctttta gtacaccgga ttcagaacca acaacctctg tattacaagg cacggcgcag
     3901 tgccattaag gataaaaaaa tgaaaagagc gcctcttata acaggacttt tgttgatatc
     3961 cacatcctgc gcttatgcct cctcaggagg gtgtggagct gacagcacta gcggtgcgac
     4021 aaattacagc agtgtggttg atgatgttac ggtgaaccag acagataacg tgacaggacg
     4081 ggagtttacc tctgccacgc taagtagcac taactggcaa tacgcctgta cctgctctgc
     4141 gggtaaggca gttaaacttg tctatatggt cagccctgta cttaccacca ctggacatca
     4201 agcaggatat tacaaactca atgatagtct ggatattaaa accacattac aggcgaatga
     4261 cattccagga ctcgtgactg accagaccgt ttctgttaac acccgattca cacagataaa
     4321 aagcaacacc gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg
     4381 ttatggaccc gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt
     4441 tctcggctcg atgaccattc cgaaaacgga tattgccgtc attaaaggcg catgggtcga
     4501 tggaatggga agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg
     4561 aaatctcacc gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt
     4621 tggattcatc aatggtcaga agtttaccac ccgcaatgcc atgccagatg gttttactcc
     4681 agtagacttt gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca
     4741 aatgcgcatc gacggtacaa ctggggtagt agaccagtac aacctggtcg ccagacgaag
     4801 aagttcagac aatgcgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc
     4861 aaatattcct tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccatcaa
     4921 catgcgcgcc tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg
     4981 caccgccacc attaccgtca tcgtgcggta aaagaagacg ttgcgttttg attaacgcaa
     5041 cgcttcggca ctcttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca
     5101 cgccgtgaat ttctgccgcc agcgccgccg cttttttcaa cggcagttct gcctgtagca
     5161 gcgccagcgt gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga
     5221 taatcagcac catttcgcct ttgcgacggt tttcatcttc ttttacccac gccagcagct
     5281 cgccaacggg cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca
     5341 cgtagcggga ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg
     5401 tggattcata aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc
     5461 ggccttttga tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg
     5521 cggcgcttaa cgcagtgata gcagcacacg gcccgggtag cggcaccacg caaatccccg
     5581 cttcacggca ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat
     5641 cggaaaccag cgcaatgttc tgcccttctt gcagcttcgc cagcagcgtt tcggcttttt
     5701 gttgttcgtt atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca
     5761 ataaaccggt gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta
     5821 acgcacgctg ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc
     5881 cctgagaatt atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat
     5941 attgagcatt gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg
     6001 tttgaaagcc gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg
     6061 cacccatact cccgatcagt ccactgctta tatgcagggc acgacgcagg ctgattctgc
     6121 cttttatctt cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact
     6181 cgccattcgt gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca
     6241 actaccgcaa gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat
     6301 taaactggcg cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc
     6361 cgatttagaa caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg
     6421 gcgtccttcc attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa
     6481 agaaaaacag cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca
     6541 ggcgaatacg ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca
     6601 gcgcgtctgg tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg
     6661 gcaaaaacgt tatccgaaca atccgggcgc gaaaatgctg ccaacgccgt tggttaacgt
     6721 aaaagcgttt aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca
     6781 ggcagcggta tttggtcgca ctattcagca aggctttgaa gcggcgaaaa atatcggcac
     6841 tcagccagtg gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca
     6901 gccgcaaacc gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg
     6961 tgaacagcct gcggcacagc cagcgcctgt aagcaccccg gcagcaacca ccacagcggt
     7021 aagcgcgcca gcgaatccat ccgcagaact gaaaatctac gatacctcat cacaaccact
     7081 tagccagatc ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct
     7141 gaaaaataac gttgaagagt tgcttaagag caacaccccg ctgaacgtgc tggcactgaa
     7201 ccagccggag aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt caccggaaga
     7261 cgaagcgcgc gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct
     7321 gatcccacgc agttcattgg gcgatcgcgt agccaatgcg tttgcgcagg agtggcagaa
     7381 actgggcggc ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg
     7441 tgttaacggc ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac
     7501 cgatgaccag ttcaccaata atggcggtcg tgtcgatgcg gtgtacattg tggcaacgcc
     7561 gggtgaaatc gcttttatca aaccgatgat cgccatgcgt aacggtagcc agagcggtgc
     7621 aacgctgtac gccagctccc gcagtgcgca agggaccgct ggcccggatt tccgactgga
     7681 gatggaaggc ttgcagtaca gcgaaatccc gatgctggca ggcggtaatc taccgttaat
     7741 gcagcaggca ctcagcgcgg tgaataacga ttattcactg gctcgcatgt atgcgatggg
     7801 cgtcgatgcc tggtcgctgg caaatcattt ctcacaaatg cgccaggttc agggttttga
     7861 aatcaacggt aataccggaa gcctgacggc aaacccggat tgcgtgatta acaggaagtt
     7921 atcatggcta cagtaccaac aaggtcaggt agtccccgcc agttaaccac caaacagacc
     7981 ggcgatgcgt gggaagtaca agcgcgtcgc tggctggaag gcaaaggact gcgatttgtc
     8041 gccgctaacg tgaacgagcg tggcggcgag atcgatctga taatgcgtga aggccggacc
     8101 accgtttttg tcgaggtacg ctatcgccgc tctgcgcttt atggcggcgc ggcagccagt
     8161 gtgacccgca gcaaacaaca caaattatta cagactgccc gcttgtggct cgcgcgtcat
     8221 aatgggagtt ttgatactgt ggattgccgg ttcgatgtgg tagccttcac cgggaatgag
     8281 gttgagtgga ttaaggatgc ctttaatgac cactcataat taaggtttaa ggattagcgt
     8341 gcaagaaaga attaaagctt gcttcactga aagcattcaa actcaaattg cggcggcaga
     8401 ggcgcttccg gatgccatct cccgtgcagc catgacgctg gttcagtctc tgctcaatgg
     8461 caacaaaatc ctctgttgtg gtaatggaac ttccgctgcc aatgcacagc attttgctgc
     8521 cagcatgatc aaccgtttcg aaacggagcg gcccagctta cctgccattg cactaaatac
     8581 tgataatgtt gtcttaacgg cgattgccaa cgatcgctta catgatgaag tgtatgcaaa
     8641 acaggtgcgg gcgctgggtc atgcgggaga tgtattgtta gccatttcca cccgtggcaa
     8701 cagccgcgat attgtaaaag cggtagaagc tgccgttacg cgtgatatga ctattgtggc
     8761 attgaccggc tatgacggcg gcgaacttgc aggtttgtta gggccacaag atgtggagat
     8821 ccgcattcct tcgcatcgta gtgctcgcat tcaggaaatg catatgctga cggtaaattg
     8881 cctgtgcgat ctgatcgata acacgctttt ccctcaccag gatgtttaag gagaatacat
     8941 gaaggcatta tcgccaatcg cagtccttat ttccgcgctg ctgttgcaag gttgtgttgc
     9001 cgctgccgta gtgggtaccg ctgctgtggg taccaaagcc gcaactgacc cacgcagtgt
     9061 cggcacccag gtggacgatg gtaccctgga agtgcgcgtg aacagcgcat tgtcgaaaga
     9121 cgaacagatt aagaaagaag cccgtattaa tgtcacggcg tatcagggca aagtgctgct
     9181 ggttgggcag tcaccaaacg ctgaactttc ggctcgcgcc aaacagattg ctatgggcgt
     9241 agacggtgcc aacgaagtgt ataacgagat tcgtcagggc cagccgattg gtctgggcga
     9301 agcgtctaac gatacgtgga tcaccaccaa agtgcgttcg cagctcttaa ccagcgacct
     9361 ggtgaaatcg tccaacgtga aagtgaccac cgaaaacggt gaagtgttcc tgatggggct
     9421 ggtgactgaa cgtgaagcga aagcggcggc agatattgcc agccgggtga gcggcgtaaa
     9481 gcgggtaacc acagcgttta cgtttattaa atagcagccc cttgtaatgc ctgatgcgac
     9541 gcttgccgcg tcttatcagg cctacaacga tgcaaaccgt aggtcggata aggcgtttac
     9601 gccgcatccg acatgttttc cctcaaatca gaacaacagc gccagcccgc cgacaatcac
     9661 accagacact gccaccatcg cccctgtcag ccataaggct ttcgctggga acgctttgcg
     9721 cagcataatc agtgacggca aactcaccgc cgggagcgtc atcaacaatg ccagcgccgg
     9781 agcagttccc atacctgcca gcatcatcgt ttgtacaatc ggaatttctg ctgccgtggg
     9841 aatgacaaac aagcatcctg ctaccgccat cgccatcacc cacatcaggc tgttatcgac
     9901 agcaccatcg gcatggggga ataaccagac gcgagcggca cccaacacca gtactgcaag
     9961 gatgtaaacc gggatcgtac tccagaaaag cgtccatagc gccctgcccc agcggctaaa
    10021 aaaaccgccc tgtgcttccg gtatgtcaat ttcgaccggt gcctgcgttt gcggtgtttc
    10081 acgcacccat ttttgcacca gcgtcgcaat cagcaacacc atcaccagcc cggccaccag
    10141 acgaatcgcc gcaaaacccc agccgaggac aaaccccata aacaccagcg tcgccgggtt
    10201 taacaccgga ttgcccatcc agaatgccag cgcaccgccc atcgacacct gttggcgacg
    10261 cattcccgcc gcgaccggag ccgcacagca ggtacacatc atgcccggca gcgaaaacag
    10321 cgttcccagc agcgtgccgc gaaagcgcga ttgcccaagc gtacgcaaca accagtcacg
    10381 cgggatcagc acctgaatca acgaaccgag gatcaccccc agcactgccg ctttccatac
    10441 cgcgaggaaa tagatcatcg cgtaatccaa cgccgcctgc cacggattag catccgcctg
    10501 cgcaaggata gatttaccga tactgtgggt ttcggcagca gtaaacgctt tgccgtagta
    10561 aggttgccat ttcacatacc agagaccggc aatgacaacg agaaagaaaa gcgcgggttt
    10621 ccaccactga atgggcgttg ccgcctgaga tgaagactga ccagtcatag cattccccag
    10681 gaataagtta tgataattga gcgcgtgaat attacgctca ctatcaattc ttgggaataa
    10741 ttattcagct cttttgcgta attctgaaga gcttaaaatc gtcacacctt catgcccggg
    10801 ccgcatcgcc tctgccagca tgacccgcgc cacgtctctc gcatcaatgg atttccagtt
    10861 accgggtaat aaacggaaca gcggcgcaaa aagcgtttcg ttcatccgct gtttgctacg
    10921 atcgcccagt aacatcgacg ggcgagcaat ggtcaatttc ggccagttct gggcgattaa
    10981 tgcttcttcc atctcccctt tgacgcggtt atagaaaaac ggcgagtggg cattggcacc
    11041 catagcactg accaccagca tatgctgcgc gcccagtcgc cgcccggtta atgcggtatc
    11101 cactaccagc gtgtaatcgg catgaataaa cgcctctttg ctccccgctt ctcgccgcgt
    11161 ggtgccgaga caacaaaaca caatgtcgat gggatcggtg acctgcgcca gcgcgtcagt
    11221 cagttgcgga tcatggggat taaacacccc gggcatatcg cccaacggac gtcgcgtcgg
    11281 cgcggctatg gcgttaactt tcggttcgtt aatcaacatc cgcagcaggt gaccgcccac
    11341 cagccccgtt gcgcctgtaa tcagtacctg actcatcttc gctcctttac agaattgtct
    11401 gccttgcgct ccacggctca tgcaccaggc ttaatagacc atgaggtaat tatccccgat
    11461 tgtgggaaat tcgcctcatc caatgcaaca acgcggagga agcatgagta agaaaattgc
    11521 cgttttaatc actgatgaat ttgaagattc agaatttact tcacccgcag acgagttccg
    11581 taaagccgga cacgaagtga ttaccattga aaaacaagcg ggtaaaacgg tgaaaggcaa
    11641 aaaaggagaa gccagcgtga ccatcgataa atccatcgat gaagtgacgc cagcggagtt
    11701 tgatgccctg ctgctaccgg gcggccattc accggattat ctgcgtggcg acaaccgttt
    11761 tgtcaccttt acccgtgatt ttgtgaatag cggcaaacct gttttcgcca tctgtcacgg
    11821 tccgcagctg ctgatcagcg ccgatgtgat tcgggggcgc aaactgaccg cggttaaacc
    11881 gatcattatt gatgttaaaa acgcgggcgc ggaattttac gatcaggaag tcgtggtcga
    11941 taaagatcag ctggttacca gccggacacc ggacgatctg ccagcgttta accgcgaggc
    12001 gttacgcctg ctcggtgcct gagccgcgta gccaaatcat ttttttacca aagcccagcg
    12061 tgttgtcggt gaatttgatt tcatcgagcc ggatttccca caccggggcc gacagcattc
    12121 tggcaaccgg aaagcgacga ttgtacgcct tgcgcgcgag gtcgctttcc tcaccttcca
    12181 gcctgcggat ctcaccttta aactgcacgc cgcgaattaa cgctaccgtt ttcggctgac
    12241 cgtttaccgt tccggcaacc gcagcctgcg gcccactcat ctgcgcgtgg cgcgtttttt
    12301 cttccgtcaa aatgtagaag gcgactttct gcgcatcaaa cagataaaaa gcattagcgc
    12361 accagagttc cccttcctgc tgcacacacc aggtgacaac atgttgtttt gccaaccaac
    12421 ggctgatggt gatgagtgtt tccattgctg ttctctttta tactgtgggc ctgaccttaa
    12481 catgcgacgc agacgatgac accctggttt ctttacttga tccgtaccgc cgacaataag
    12541 ctttataccg ggatcaccac ggatgtcgaa cgccgctatc agcagcacca aagcggcaaa
    12601 ggggcgaaag cactgcgcgg gaaaggagaa ctgacgctgg cgttttctgc gccagtgggc
    12661 gatcgttcgc tggcgttacg ggcggaatat cgcgttaagc aactgaccaa acggcaaaaa
    12721 gagcgtctgg tagcggaaag cgcagggttt gcagagctgt taagcagtct gcaaaccccg
    12781 gagattaaaa gcgattgaaa tgctcgtgat actcaaccag accagttacg ccattcaacg
    12841 cgtcatccgc taaacgatgt acctggaagg cgctttcagt gcctggccag cggcaacgca
    12901 ggtcgtgatg tgccgccagt tcaaagccaa aacggctgta cagcgccgga tcgcccagcg
    12961 tcaccactgc ggcatagccg aactcattaa gcgaatcgag tccttcatag accagttggc
    13021 gtgccagccc ctgtccacgg tatttttcat caaccgccaa cggtgccatg ccgacccatt
    13081 gcaggtcttc gccctgcaca tcaaccggac taaatgccac atagccaatg acctgacctt
    13141 cgtcatctgt cgccaccagc cccagcgtca gaaagccatc ttcacgcaga tcgtgaacca
    13201 gcttcgcttc cgcatcactt tcgaatgagc gacgtagcag ggcatcaata cccggcgcat
    13261 caatgggaat ttctactcga attagcatgg ttcacctacc gatgtctgtt tggtttcagg
    13321 cgcggttttc attcccgcct caacaaaatc cgccagttgc agcagcatca tgcgtaaggc
    13381 tttcggcatt tgctccagtt caatggcgtc catcaggttt ttcacataca gccccagctc
    13441 cgtatcgcct tcaatcacca gccgacgctg gaagaagagc gtatccggat cttgtttacg
    13501 cgccgcaatc atcagcagat cgctggcgtc ggcactaaaa ctcacatcag cttgcgcgtt
    13561 ctggctgacc accagtttgc cattcaccac cgaggtaaac cattgcaggt caatatcacg
    13621 cacatgaata cttaaccagc ggccttcaag aaactccagc tcgccatcat ccagcgcctg
    13681 acggaattgc cagcttaaga cctgctcaag aacctggcgt tttagcgcaa atggcgtcag
    13741 ttttaccggt acactcaaca gagatggccc caaatgcaca atacgggaac gcagtttatc
    13801 caacacgagt tttactccct gtttcaacaa tcatcctatt ttgccatatc agaaaaataa
    13861 catagcggta taaatcaaca attccatatg aaattgctgc taccaccaat acaactttaa
    13921 ctgccttaaa tcaaaaattg tcgcagcaag gttaactaaa atcccagttc gttaacattt
    13981 ttgcgttttg atagcgcaac cttcaggaaa aattatggag ctgctctgcc ctgccggaaa
    14041 tctcccggcg cttaaggcgg ccatcgaaaa cggcgcagat gctgtttata tcgggctaaa
    14101 agatgatacc aatgcccgtc acttcgccgg ccttaacttt accgagaaaa aattgcagga
    14161 agcggtgagt tttgtccatc aacatcgccg caaacttcac atcgcgatta acacttttgc
    14221 gcatccggac ggttacgccc gttggcagcg tgccgtggat atggcggcgc agctgggtgc
    14281 cgacgcgctg atcctcgccg acctcgccat gctagagtac gccgccgagc gttacccgca
    14341 tattgagcgt catgtgtcag tgcaggcttc ggcgaccaat gaagaggcga ttaactttta
    14401 tcatcgccat tttgacgttg ctcgcgtggt gctgccgcgc gtgttgtcga ttcatcaggt
    14461 gaaacaactg gcacgggtca cacctgtacc actggaagtc tttgctttcg gcagcctgtg
    14521 cattatgtcg gaaggtcgtt gctatctgtc gtcgtatctg acgggtgagt cgcccaacac
    14581 cgtaggcgcg tgttctccgg cccgtttcgt gcgctggcaa caaacgccgc aggggctgga
    14641 atcccgcctg aacgaagtgc tgatcgaccg ttatcaggac ggcgaaaacg caggttatcc
    14701 gacgctatgt aaagggcgtt atctggtgga cggcgagcgc tatcacgcgc tggaagaacc
    14761 aaccagtctc aataccctgg aactgctgcc ggagttaatg gcggcgaata ttgcttcggt
    14821 gaaaattgaa ggccgccagc gtagcccggc gtatgtcagc caggtggcga aagtctggcg
    14881 tcaggctatc gaccgttgta aggccgatcc gcaaaacttc ataccgcaaa gcgcgtggat
    14941 ggagacgctc gggtcgatgt ccgaaggcac gcagaccacc cttggcgcgt atcaccgtaa
    15001 atggcagtga gaagagcaat gaaatattcc ttagggccag tgctgtggta ctggccaaaa
    15061 gagacgctgg aagattttta tcagcaggcc gccaccagca gcgccgacgt gatttatctt
    15121 ggtgaagcga tatgcagcaa gcgccgggca accaaagttg gcgactggct ggagatggca
    15181 aaatcgctcg ccggaagtgg caagcagatt gtgctctcca cgctggcgct ggtgcaggca
    15241 tcctctgaac tgggcgaact gaaacgctat gttgagaacg gtgagtttct gattgaagcc
    15301 agcgatctcg gcgtggtgaa tatgtgcgct gaacgcaaac tgccgttcgt cgccgggcac
    15361 gcgctgaact gctacaacgc ggtgacgctg aaaatattgc tcaaacaggg catgatgcgc
    15421 tggtgtatgc cggtggagct ttcccgcgac tggctggtga atctgcttaa tcagtgcgat
    15481 gagctgggca ttcgcaacca gtttgaagtg gaagtcctga gctacggtca tctaccgctg
    15541 gcctactccg cccgctgctt taccgcgcgt tcggaagacc gcccgaaaga tgagtgtgaa
    15601 acctgctgca ttaagtatcc gaacgggcgc aacgtgctgt cgcaggaaaa ccaacaagtg
    15661 tttgtactca atggcattca gaccatgagc ggctacgttt acaacctcgg taacgagctg
    15721 gcatccatgc aggggctggt tgatgtggtc cgcctgtcac cgcagggtac tgacactttc
    15781 gcgatgctcg acgccttccg cgctaatgaa aatggcgctg cgccactacc gttgacggca
    15841 aacagcgact gtaacggcta ctggcggcgg ctggccgggc tggagctgca agcctaagta
    15901 aatatgctcg tcatacttca agttgcatgt gctgcatctg cattcgttca ccccagtcac
    15961 ttacttatgt aagctcctgg ggattcactc tcttgtcgcc ttcctgcaac tcgaattatt
    16021 tagagcacag ctcactttgt taacaacttt aactactctt taatgcagta ttaaagatta
    16081 atcggtaaca aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct
    16141 ggcccccatt cccgaaggtt cttcagcgcg agaagcattc tcccactctc tcaatctcgc
    16201 ccgtctggct gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac
    16261 tggcattgcc agtgctgcca cgtcggtatt gattggctat ctggcggcga ataccaccac
    16321 gctgcatctg gggtctggcg gcgtgatgtt gcctaaccac tcaccgttgg tcattgccga
    16381 acagttcggc acgctcaata cactctatcc ggggcgaatc gatttggggc tgggtcgtgc
    16441 gccaggtagt gaccaacgga caatgatggc gctgcgtcgc catatgagcg gcgatattga
    16501 taatttcccc cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc
    16561 gcatgtgcgc ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag
    16621 cctttacagc gcgcaactgg cggcgcagct tggtctgccg ttcgcgtttg cctcacactt
    16681 cgcgccggat atgttgttcc aggcgctgca tctttatcgc agcaacttca aaccgtcggc
    16741 acgactggaa aaaccatacg cgatggtgtg catcaatatt atcgccgccg atagcaaccg
    16801 cgacgctgag tttctgttta cctcaatgca gcaagccttt gtgaagttgc gccgtggcga
    16861 aaccgggcaa ctgccgccac cgattcaaaa tatggatcag ttctggtcgc cgtccgaaca
    16921 gtatggtgtg cagcaggcgc tgagtatgtc gttggtaggt gataaagcga aagtgcgtca
    16981 tggcttgcag tcgatcctgc gcgaaaccga cgccgatgag attatggtca acgggcagat
    17041 tttcgaccac caggcgcggc tgcattcgtt tgagctggcg atggatgtta aggaagagtt
    17101 gttgggatag tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt
    17161 agacctgata agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca
    17221 ccgctgatta ctgatacacc ggcagtaaat taaagctcga taaaatatgg accagtgcgt
    17281 tgccaacgcc aaacaccaga atcagcgtaa tcatcggctt gccgccccag acgcggaatt
    17341 tcgggctgcc aaagcgttta cgcgatgcgc gggctaacag cgccggaaca attgccgccc
    17401 agatggtagc tgctaaacca gcataaccaa tggcgtacag gaatccgttc gggaacagta
    17461 gaccacccac aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgc cccagggctg
    17521 agtcatcgaa accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga
    17581 acgaactcgc taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt
    17641 tcagcacgcc gcttaatgcc tgtaccagca catcaatatt accgcccttc tctgcaatac
    17701 cgataaactc cggacgcggg atgttaccca tcgtcgccag caaccagatg gtatacagcg
    17761 ccagtgccat cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt
    17821 aatacttcat caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca
    17881 atgtcatcag cagatacggc gcataagatg cattgctttc tgcaacgttg aacaatgtcg
    17941 caggctgcac atgccccaac aggctgccaa aggtgaggaa gaaggtaatg actttcgccc
    18001 ccagcacaat cgccgtcatg cggctgacgg ccttagtact caaccacacc acaaacgcta
    18061 ccagcagtgc aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga
    18121 aggtgtgatg cagaattgaa ccactggcag aaatataggc ataggtcagg atatagagca
    18181 caaaggcaat ggaaataccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga
    18241 tggtgtcaaa actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg
    18301 aatgcagcat acagaaccag gtaaagatca gcgccgccat tgaccagaaa aaccacgccc
    18361 cggacatgac cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca
    18421 ccacgccgcc aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt
    18481 ctctccagtg aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacacgag
    18541 tacaaaagac aaaaaaaaag ccccgatggt aaaaatcggg gctgtatata ttattttaca
    18601 gattgtgttc gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag
    18661 agtcatcacg acgcggagcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac
    18721 cacggccacc ttcacggcgt tcaccactga agttacgacc gccttcacga cgttcgccac
    18781 cgaaaccacg accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta
    18841 actgcatgtt catcggcttg ttgagaatgc gagtacgcgt aaagtgttgc agcacttcac
    18901 ccggcatacc tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccaa
    18961 tgtaacggct gctgatgtcg ccttcgttag caatcgcacc aacgatatga cgaacttcaa
    19021 caccatcatc gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt
    19081 cacgacgcgg acgatcttca cggtcaccac gcgggccacg gtcgttacga tcgcgcggac
    19141 cacggtcatc acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggtacga
    19201 tcagagtacg ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca
    19261 gctcttcacc ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat
    19321 cgctgctttc cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca
    19381 gcagttctgc gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt
    19441 tgcgcagcag acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg
    19501 cacgaccggt acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt
    19561 taactaccag gctgatacgc tcaacgtcca ggccacgggc tgcaacgtcg gtcgcaatca
    19621 ggatgtccag acgaccatct ttcaggcgtt ccagtgtctg ttcacgaagc gcctggttca
    19681 tgtcaccgtt cagcgcggcg ctgttgtagc cgttacgctc aagagcttca gccacttcca
    19741 gagtcgcgtt tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga
    19801 aacgtaccag tgcttcgttt ttgcgcatac cccagacagt ccagtagctc tggctgatgt
    19861 caggacgggt agtcacgctg gactgaatgc gcacttcctg cggctctttc ataaagcggc
    19921 gggtaatgcg acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt
    19981 ccgggatctg cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt
    20041 cagcttcatc cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt
    20101 ggtccagcag acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta
    20161 attgcacgtc ataacgctgg ccgccgtaca gagcaaccac atttacgccg cgcatgtgtt
    20221 tagagaaatc cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca
    20281 ccagaatctg tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg
    20341 ctgcagtttt tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat
    20401 gtggaataca ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt
    20461 caaggatagg agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt
    20521 acgtgtgcct caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca
    20581 attttcattg aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct
    20641 cacccgttaa ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg
    20701 ctggtccgat tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc
    20761 gtatcggtgc tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt
    20821 ggcgctgtcc aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact
    20881 gagatgctca gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt
    20941 ttgttcgcta atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc
    21001 cgatttttcg aagtgctgtt tcaacacttc tttagcctgc ttctcatcga gcttctgctc
    21061 ggcgagataa agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag
    21121 cagatcatct tgcgctaact tgtcacgacc gccgtaatat aatgcgatcc cgcgattcaa
    21181 gtgcgcgtag ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc
    21241 atcaaaattg cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc
    21301 cggtcggatt gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact
    21361 atcatacaac actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg
    21421 actggcaagg atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa
    21481 tggtaccgcg aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag
    21541 tgctgtcgca acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg
    21601 gttgaatgaa cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc
    21661 cgccgcagcg gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg
    21721 tgctgcagca ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg
    21781 gcgatcaact tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac
    21841 tttctcaacg cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc
    21901 gatggcaaca aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc
    21961 cacttcgatt tctgcagtga tctcttcgat acgacgaata gcgtgtttcg ctttctcgcc
    22021 gtcggtcgct gcgatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc
    22081 ttcggtcagg gcacggataa cagaaccgcc tttaccgata acgtctttga tcttgtccgg
    22141 gttgatcttg atggtatgga tacgcggtgc gaactcagag atatcgccac gcggcgcgtt
    22201 gatcgcctgt tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc
    22261 aacctgcatg atctctttgg tgataccttc aattttgata tccatctgca atgcagagat
    22321 accgtcgcgg gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa
    22381 aatgtcagac agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc
    22441 aacggcagct ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga
    22501 agccatagaa gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg
    22561 gaatttgtcc atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc
    22621 aatttcacga cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa
    22681 gttgtagtgg aacaggaagg tgtcggtacg ttcgcccatc agttcatcaa gaacctgcgc
    22741 gtcacgagca gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag
    22801 cgcagaaccg tgagtacgcg gcagcacgcc agtacgcaca tccagaccac gaatcatatc
    22861 tttttcacga ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt
    22921 ctcgatagcg tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag
    22981 cagcgtcgcg atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc
    23041 ggtgatgcgg taagcatcac tcagacgagc ttcagccagt gcagcaacgc gcgcgttcag
    23101 cgcttcgttt accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag
    23161 ttcattgatg ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag
    23221 catctggtct tcgctcagca attcagcttc agattcaacc atcagtacag cggcttcagt
    23281 accagcaaca accagatcca gtttgctctc tttcagctcg tcctgagtcg ggttcagtac
    23341 gtactggtca ttgatgtaac ctacgcgggc agcaccaatc ggaccattga acggaatacc
    23401 agacagagac agtgctgcgg aagcaccaat catcgcgacg atatccggat taacttgcgg
    23461 gttaacagaa accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag
    23521 cgggcgaatc gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc
    23581 ttcacgacgg aagaagctac ccgggatacg accagcagcg taagtacgct cctgatagtt
    23641 aacggtcagt gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac
    23701 gaataccgcg gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat
    23761 gccggtttcc agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag
    23821 caatgtaata tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga
    23881 tcttctgcgc atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct
    23941 ctcattagcc gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt
    24001 ccagtgaatt gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac
    24061 tcgcaagaat tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc
    24121 tttacgtttc aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg
    24181 acggctgtgg tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc
    24241 agtcagcagt gctacctgaa cttcggtaga accggtgtcg tttgcgtcac gaccaaactc
    24301 agaaacgatt ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata
    24361 gaatgaaagg acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa
    24421 acaatttacg cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg
    24481 gttaacatta cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt
    24541 caatttcgcc cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt
    24601 ccagcggcgc accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta
    24661 acggaagatt caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat
    24721 caagtaactc cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat
    24781 gttccagggt caccatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa
    24841 taacatgcgc gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag
    24901 tgccttttga gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca
    24961 attcataaac ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt
    25021 cgtacagttt tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat
    25081 cgccacggaa agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt
    25141 cttcaacgat ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga
    25201 cccgatagcg tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc
    25261 aaattggcaa catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg
    25321 cgttataaat ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat
    25381 ccagcaacaa aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc
    25441 cttgctgtcg tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag
    25501 gtttgacatg cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac
    25561 gatacgcagg cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa
    25621 cgctttgatg cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac
    25681 tttggcatac gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag
    25741 gcgaggatct ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc
    25801 tacgcgctgc gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc
    25861 tgttagcccc tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag
    25921 acgcgccagc gtcgcatcag gcagtccatg caggatgcgg cgtaaacgcc ttatcccgca
    25981 tggaacccta aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga
    26041 tcacatcgcc agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt
    26101 tacggacttc gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga
    26161 taaccacgtt gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa
    26221 ccatacagcc tgcgatggca ccaaatttcg gtgatttgaa cacgtcacga acttccgcca
    26281 gaccgataat ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt
    26341 cgtcaatcag gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca
    26401 ctttacgtgc agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg
    26461 cagccagggt ggcgtcggtt tcggtgatac cccctacgcc agaaccgatg atcttcactt
    26521 taacttcgtc agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta
    26581 cgtctgcctt caggacgata ttcacttcgt gaacttcgcc ttcggtcatg ttggcaaaca
    26641 tgttctcgag tttagatttt tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac
    26701 gatagagtgc aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg
    26761 cagccggaac gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca
    26821 cttcctgacc cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa
    26881 cgatatcgcc cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac
    26941 ctttatcgag gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttta
    27001 gctccagaac ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccggtacccg
    27061 ccttcgcaga tacgtgtacg aactggcttt caccgcccca ctcttccggc aggatgccat
    27121 actgggagag ttcgttctta acacgatccg gatcggcttc tggtttatcg atcttgttca
    27181 ctgcaaccac caccggtacc tgcgccgctt tcgcgtgctg gattgcttcg atggtctgcg
    27241 gcatcacgcc gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac
    27301 gtgcacgcat tgaagtaaac gcagcgtgcc ccggggtgtc caggaaggtg atcatgccgt
    27361 tttcagtttc aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg
    27421 ccactttcgt tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca
    27481 tgatggtcac aaccggcgcg cgcggttcag ccgcagcccc cgtgtcacgg tcgctcatta
    27541 ccgcctcttc cagctcgttt tcacgacgca ggataacttt gtggcccatc tcttcagcaa
    27601 ccagctgtgc ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca
    27661 tcatcgcttt gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg
    27721 tgatggtttc gccgatcaca acgtcacggt taacggcctg agctggcttc tggaagcctt
    27781 gctgcaggga agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt
    27841 cttcacgatc agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg
    27901 cgttacgacc acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc
    27961 gagcatgttg agaagttgtg acatgataat cgctggaatc ttcagtcggt tccgcgttat
    28021 cagtccattt gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt
    28081 cttcgagttt acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct
    28141 cacggcgggc tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt
    28201 tgtctttttc cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg
    28261 cggcctcacg ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac
    28321 gggcttgctc ttccgcttca cgctgcgctt gctcttccgc tgcaaggcgt tcagcctctt
    28381 gcggatcgcg tttcacaaag gtgcgtttct tgcggacttc gatttgtacc gatttgcttt
    28441 ttccaccggt accaggaatg ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt
    28501 ccgggcctga atttttctga ttcaggtggt caatcaaagt ctgtttctct tgtgcagaca
    28561 cagagtcgtc agcagacttc cggatacctg catcagcaaa ttgctgtacc aggcgttcca
    28621 cggaggtctg tcgctctgcg gccagcgttt taatcgttac atctgtcatg ctgttccttc
    28681 ctgctacagt ttattacgct tcgtcaccga accagcaaat attacgggca gccataatca
    28741 gtgctccggc tttttcgtcg gtcaaccctt cgatatcagc cagatcatca atgccctgtt
    28801 cggcgagatc ttccagcgta caaacgccac gggcggccag tttgaatgcc aaatcacgat
    28861 ctaccccttc aaggttcagc agatcgtcag ccggtttgtt atcaccgagg ctttcttcct
    28921 gggcctgtgc aatggtggcc agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg
    28981 gctcatcaag gccttcgatt tccaacagct ctttcatcgg aacataggcc agttcttcca
    29041 gcgtcgagaa accttcttct accagaacag tcgcgaagtc ttcgtcgatg tcgagatatt
    29101 tggtgaaggt gtcgatcgct gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa
    29161 cggtcattac gttgagttcc caaccgctca gctgcgaagc cagacgcacg ttctgaccgt
    29221 tacggccaat cgcctgcgcc aggttaccgg cttcaacggc gatatccatg gtgtgtttat
    29281 cttcatccac cacgatagaa gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg
    29341 ccgggttatc atcccacagg acgatatcga tacgctcgcc gcccagttca gtagaaaccg
    29401 cctgaacacg cgcgccacgc atacctacac aggcacctac cggatcgata cgtttatcgt
    29461 tggttttcac cgcgattttc gcacgagaac ccggatcgcg agccgctgct ttaatttcaa
    29521 tcacttcttc gccgatttct ggcacttcaa tacggaacag ttcgatcagc atttccggct
    29581 tggaacgagt gacgaacagt tgcgcgccac gcgcttccgg gcgaacggaa tagagcacgc
    29641 cacgaacgcg gtcgccaggg cggaagtttt cacgcggcag catatcttcg cgcaggatca
    29701 cggcttcagc attgttgccg agatccaaag agatgttgtc gcggtttact tttttcacca
    29761 cgccggtgat gatttcacct tcgtgttcac ggaactgatc aaccaccatc gcacgttcgg
    29821 cttcacgcac tttctgcacg ataacctgtt ttgccgtctg ggtagtgata cggtcaaagg
    29881 taacagactc aatctgatct tcaacgtaat cgcccaggtt caggctttca tcttcataac
    29941 gtgcggcttc aagggtgatt tccttggtcg gctgggtgac ttcatcaaca actaaccagc
    30001 gacggaaggt gtcaaaatca ccgcttttgc gatcgatctg tacgcggacg tcgatctctt
    30061 gttcatattt tttctttgtt gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct
    30121 cgcgaggtag cgccttttca ttggatacgg cttcaactac agccaaaatt tctttgttca
    30181 tcgcgggctt ttcacctcat ccagactatt aaaagtgggg aaccaggttc gccttctgga
    30241 tattactcag cgcgaacact tcatctttac cttcgacggt aactgtgatc atttcaccgt
    30301 ctaccgcttt gataacgccc tgccatttac gacggttttg taccgccata cggagaacca
    30361 gagtcacctc ttctccgaca aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga
    30421 gacccggtga ggagacttcc aggttataag caacggtgat gggatcttca acatccagca
    30481 cagcacttac ctggtggctc acatcagcac aatcatcaac attgatgcca tcttcactat
    30541 caatatagat gcgcagtgtg gatgtgcgac cgcgaataaa ttcgatgcca accagttcaa
    30601 aacccagggc ctcaactggc gcagtaatca tctctgttaa tttttgctct aatgtggaca
    30661 agcccacccc caagacataa aaaaagggct taaagcccag ttattctgta gtcagataac
    30721 aaaaaacccc gataaatcgg ggctttatat aactgaaccc tataaccgca actgcggtct
    30781 ggagcacttt ccagaaggat tttttcaaat cccactacga aggccgaagt cttcacagta
    30841 tatttgaaaa aggactctaa gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct
    30901 tcgggttatg agcccgacga gctaccaggc tgctccaccc cgcgcctgaa acgtggcaaa
    30961 ttctactcgt tttgggtaaa agatgcaaat actgctggga tttggtgtac cgagacggga
    31021 cgtaaaatct gcaggcatta tagtgatcca tgccacattt tgtcaacgtt tattgctaat
    31081 cacgtgaatg aatatccaat tcactttcat ttgttgaata cttttgcctt ctcctgctct
    31141 cccttaagcg cattatttta caaaaaacac actaaactct tcctgtctcc gataaaagat
    31201 gattaaatga aaactcattt attttgcata aaaattcagt gagagcggaa atccaggctc
    31261 atcatcagtt aattaagcag ggtgttattt tatgacgacg attctcaagc atctcccggt
    31321 aggtcaacgt attggtatcg ctttttctgg cggtctggac accagtgccg cactgctgtg
    31381 gatgcgacaa aagggagcgg ttccttatgc atatactgca aacctgggcc agccagacga
    31441 agaggattat gatgcgatcc ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct
    31501 gatcgactgc cgcaaacaac tggtggccga aggtattgcc gctattcagt gtggcgcatt
    31561 tcataacacc accggcggcc tgacctattt caacacgacg ccgctgggcc gcgccgtgac
    31621 tggtaccatg ctggttgctg cgatgaaaga agatggcgtg aatatctggg gtgacggtag
    31681 cacctataaa ggaaacgata tcgaacgttt ctatcgttat ggtctgctga ccaatgctga
    31741 actgcagatt tacaaaccgt ggcttgatac tgactttatt gatgaactgg gcggccgtca
    31801 tgagatgtct gaatttatga ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc
    31861 ctactccaca gactccaaca tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct
    31921 caactccagc gtcaaaatcg tcaacccgat tatgggcgtg aaattctggg atgagagcgt
    31981 gaagatcccg gcagaagaag tcacagtacg ctttgagcaa ggtcatccgg tggcgctgaa
    32041 cggtaaaacc tttagcgacg acgtagaaat gatgctggaa gctaaccgca tcggcggtcg
    32101 tcacggcctg ggcatgagcg accagattga aaaccgtatc atcgaagcga aaagccgtgg
    32161 tatttacgaa gctccgggga tggcactgct gcacattgcg tatgaacgtc tgttgaccgg
    32221 tattcacaac gaagacacca ttgagcagta tcacgcgcat ggtcgtcagt tgggccgtct
    32281 gctgtatcag gggcgttggt ttgactccca ggcgctgatg ctgcgtgact ctctgcaacg
    32341 ctgggttgcc agccagatca ctggtgaagt taccctggag ctgcgccgtg ggaacgatta
    32401 ttcaatcctg aataccgtct cagagaacct gacctacaag ccagagcgtc tgacgatgga
    32461 aaaaggcgac tcggtgttct cgccagatga tcgtattggt caattgacca tgcgcaacct
    32521 ggatatcacc gatacccgcg agaaactttt cggttatgcc aaaactggcc tgctctcctc
    32581 ctctgccact tcaggcgtgc cgcaggtgga gaatctggaa aataaaggcc agtaattcga
    32641 catcaaccct gcccttcagg ggtggggtta ccttgcttat cctctgtaaa ctcctttctg
    32701 agcatcacat aatcgaaaac atcatggttt gcatccacaa cccgtgagtc tcctttatag
    32761 tgtgcaatca cttcctccag cgtttgcggc tgttccggct ttgttacccc catccacgca
    32821 ttaataaggt aattattgta cgcagtggaa aagatatcgt tttctgttgt gcgctccacg
    32881 ccatccccaa gaacggggct ataccagata aacatcggga catgatatgc ctgctggctg
    32941 gcctccctgc ctccatgaaa atagacgttc ttcttcgtag ggtcgcgttc caggccgtgg
    33001 tcggcaaaat acatgaccga ggcgcggcga tcttttagta attcaaaaac ctgacctagc
    33061 aaactgtctg tgtaatgaat ggagttgtca tagcaggcat cctgatcgtc ctgcggttga
    33121 aacacggcgc tggattgcgg ataggcgcta caagccggtt catggcttcc atttaaatga
    33181 agaacaatca gtttcttttg ctgcgtcttt tgctgtaacg cttgcgataa atgcggcaac
    33241 aacaattcat caaatcctct gacatagact gtttccatgg cgcgcatggc gatactggta
    33301 actgctgtac cattctgccg aaaagcggat tgcgagctta gccagaaagt ctgaaatcct
    33361 gcttgattag ccatattaat aatgttgtcg gggtaattat gaatgtcatg actcaaaaca
    33421 gaatcagcag ttaaagaaag gggaaccgac agcgcagtgt aaggtgcgcc gcttattgct
    33481 tgattaaaca gtttgatctg ttttctttgt gcttcaactt gcggtgtcgt agagcgtgta
    33541 tatccataca aagacatatt gtcgacacgt acagactccc ccacaatcaa cacgtaggta
    33601 tcaatacctg tatccctgac tgataattga aaatacggca ccgtgtttgc aattgagagt
    33661 aatctttgat gctctttcgc tgctaaagca aaatagttga gattaaaaaa cggcgtatag
    33721 gtagcaaatc gcgacgctag tatatatgga ctgaacgctt ttttattttt tgcatcttta
    33781 taagcaaatt gacaagcgga aaataaactg cccgaaatga caatcagcaa taatattcct
    33841 gtcacttttt ttgtcggcaa ggaaacatca tattttatta ttactgccaa aaaaagaagg
    33901 gataaaaaag caaaagcaca tagataaggg atatacatcc ccagcatttt gacaacttca
    33961 tccggatcac tctgcagcac actaatcgca aatccatcat tgaaagttgt accaaaggta
    34021 caccatgaat agaggctaat actcatgtct gccgcagtca gtaacacaaa tggtgcggca
    34081 acaagcaggc gaagcggaag ggatgatata cgtttaacaa ccaggatggt caggacgaag
    34141 aacagcaatc gctgaaccat atgcgctccg gaagaggcga ctaagttcgt aataccaaaa
    34201 acaataacac aaagatacaa caaccaatga gatttaaaac ttctagcaaa tttgttgaat
    34261 actgtcatcc agaaatccaa gttcacaacc aatataaaca attatcaaca atgttcaatt
    34321 ttatatcaga catgtttttc aactctgatc catatcagac cgaaacactg ttagtttttt
    34381 ctttttaaat gatgcggcca ttgttctcag taattaaata acattatcaa tgcgtctcta
    34441 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
    34501 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
    34561 gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt caaagtacca
    34621 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcct cagaccagga
    34681 ccaaaacgaa aaaagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
    34741 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    34801 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    34861 cagctggctg agtttgttcg gttttcgccg gcgcactcag gttttcccat tcgctacctt
    34921 tattggtttt attgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    34981 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    35041 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    35101 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    35161 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    35221 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
    35281 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgctgag
    35341 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    35401 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
    35461 cacgcgaatt aacggttcgg tgccggattt acgcagcaac acgcgtccac ggttgcccag
    35521 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagtg gatcgccgct
    35581 acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    35641 gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat
    35701 gccgtcaccg gtagtagttt tatccagtag gatcacatga ccggaattct ctgcaccgat
    35761 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
    35821 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    35881 cacagcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    35941 ctgatcgcca tcgactttat tgccttcatg gtcgaccata atcacgcgat cgccatcgcc
    36001 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
    36061 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
    36121 aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
    36181 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    36241 aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca gttctgccga
    36301 atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg
    36361 cagtttggtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg ggttatgcga
    36421 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    36481 tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag
    36541 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
    36601 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
    36661 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    36721 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    36781 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
    36841 aggccagact accgctcagg cgctcggacg gcaccacgtt cagcagttgc ccaatcatcg
    36901 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    36961 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    37021 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
    37081 tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct
    37141 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
    37201 cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga
    37261 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    37321 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg
    37381 tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt
    37441 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
    37501 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
    37561 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
    37621 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    37681 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
    37741 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    37801 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    37861 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    37921 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
    37981 atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag
    38041 cagtggaccc aatttctcag agaagcccca ctgggtcacc atgttacgtg ccagattggt
    38101 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccat agatgatctc
    38161 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    38221 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    38281 aatcgtcact ttgtgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    38341 cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    38401 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    38461 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    38521 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggggcca atggtacgcg
    38581 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac
    38641 ctgacggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt
    38701 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    38761 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    38821 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    38881 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
    38941 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc
    39001 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    39061 gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca
    39121 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    39181 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    39241 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    39301 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    39361 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    39421 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    39481 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    39541 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    39601 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    39661 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    39721 ttcccgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    39781 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    39841 gccacctggc gctaatacat cacgacacat ttctaacgcc agttccacca gatacatggc
    39901 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    39961 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    40021 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    40081 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
    40141 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    40201 aagtttaaac caggcacggg aacgtaaccc ctttttctgt gcctgttgaa catatttatc
    40261 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    40321 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    40381 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    40441 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    40501 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    40561 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaactaaaa
    40621 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    40681 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    40741 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    40801 aaatgccacg ctctgttcgt tgataaaagg ccgcatagcg gcctttttcc tttctttaca
    40861 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    40921 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    40981 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    41041 cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag
    41101 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg
    41161 acagcttggc ttcgatgtct ttaatacggc cttcgcagaa accctgctgt tcacgagctg
    41221 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    41281 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    41341 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    41401 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    41461 gcaaaaaaat acccacccgg gtacaagtcc caggtcagct acaattcaca ttttgatagt
    41521 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    41581 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    41641 gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc
    41701 aactccccgc tggtgccaac cttgccctga tggtgcaaaa agtcggcgcg tcggcccccg
    41761 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacgcagaaa gtgattactg
    41821 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
    41881 aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    41941 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    42001 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    42061 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgcc gctgccatag
    42121 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaaacctggt gatatggctt
    42181 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    42241 gaggttctgc cgaagcgcaa tactgcgaac tggatgtcgt accaggcgac ctgaaccgct
    42301 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttgccgtgc
    42361 aggatggagc cagctatgcc ggggcaattc tgaaagatga gttaaaacag gcaggtatca
    42421 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
    42481 gtaaacagtc ggccccgttg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    42541 tgatcgccga tacggttttc cgcatgatag gccatgcgcg cttcaatgtg cctggaacat
    42601 ggcgggccgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    42661 gaaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    42721 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
    42781 tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    42841 gcgtggatgg aaaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    42901 gattcattac cacagcgagc gggcaacgaa tggcgtttgt acaatatctt tctggctatg
    42961 cagtagaacc ggcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    43021 tgtataaaga tatttatcag aacaattagt caaaaagaaa ccccggcaca tggccggggt
    43081 tttgctgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtca
    43141 tcatcccagt cctcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatca
    43201 tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt
    43261 tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc actcgccgca
    43321 gagatcagat aatatttatc ttcccagccc agcgcttcag caatcgcttt cgctttctct
    43381 tcagcttctg ccttatccag cagatcgatc ttattgaaca ctaaccaacg tggtttcgcc
    43441 gccagatcct ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga
    43501 tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg
    43561 tgcttcagga agcgaatgcc cagacctgcg ccttccgcag cgccttcaat cagtcctgga
    43621 atatcggcaa caacgaagct cttttcgttg tccattcgta ccacacccag acttggcacc
    43681 agagtggtaa acggataatc cgccactttc ggtttagccg ccgataccgc acgaataaag
    43741 gtcgatttac ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc
    43801 agcagcagct cacgcttatc gcccggcgta ccgttagttt tctgccgtgg tgtacggtta
    43861 actgacgatt tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga
    43921 cgctgaccgt gtttggtcat atcgcccatg gtttcaccag taccctggtc gattacacgc
    43981 gtacctaccg gcactttaat tgtgacgtct ttaccgcgct taccggtaca gtcgcggctt
    44041 gcgccattct gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg
    44101 ttcaggttct cgtcggcttc catccatacg tcaccaccat caccgccgtc gccgccatcc
    44161 gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca
    44221 cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc cgtaaatcat
    44281 tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga
    44341 tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa
    44401 aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa
    44461 aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga
    44521 gccatcgctt ccgccagggc gccatatcct accaaggtat tcagtccgca aaaaattaaa
    44581 catgcgagct gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc
    44641 gcaattgtac ataaagtgta cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat
    44701 aaaacctttt gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac cccaaagata
    44761 actccccagg tgtaatcggt gagctttgta aatatctcga ccagactggt gttaaaaaac
    44821 atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct
    44881 ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc aatcacctgc
    44941 gaagcggtcg gactcaggta ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc
    45001 ccggcggtcg ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc
    45061 ggcaacctct tcttcaccgc aagaatggca ccaaggccaa tactcgccat caagaaacgg
    45121 taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac
    45181 gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc
    45241 ataccgtatt ccctgcaaag cgcatttgcg gattaacgtc gcaccatgcg catttttacc
    45301 ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg
    45361 cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg
    45421 aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta
    45481 gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat
    45541 tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg
    45601 gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc
    45661 cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga tagtgtttac
    45721 gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt
    45781 tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca
    45841 tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa
    45901 cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat
    45961 aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa
    46021 tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa
    46081 gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcact
    46141 ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa
    46201 aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc
    46261 gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccagctga tcaatcagtt
    46321 aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc
    46381 tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt
    46441 tatccacacg gcgactctgc tgcacgacga cgttgtggat gaatcagata tgcgcagggg
    46501 taaagctacc gccaacgctg catttggcaa tgccgccagc gtgctggtag gcgattttat
    46561 ttatacccgc gcgttccaga tgatgaccag cctcggttcg ctcaaagtgc tggaagtcat
    46621 gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga acgttaacga
    46681 tccggacatc actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt
    46741 tgaggctgcc gcgcagtgtt ccgggattct ggctggctgt acgccggaag aggagaaagg
    46801 cctgcaggat tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga
    46861 ttacaatgcc gatggcgaac agttaggtaa aaatgtcggc gacgatctga acgaaggtaa
    46921 accgacgctg ccgctgctgc atgcgatgca tcatggcaca ccagaacagg cacagatgat
    46981 ccgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc aggaagcaat
    47041 gaacgcttgt ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc
    47101 catcgcagca ttacaggtgc tcccggacac cccttggcga gaagcactca tcggcctcgc
    47161 gcacatcgct gttcaacgcg atcgttaatc ccctcccttc atcccgcgca gtgcgcggga
    47221 tgattccagt aaattccata aaaacactaa ttcagcgcta taacacctga aaaaaagggc
    47281 gatgaatctt tatatattct gaatattcac actctttaca ggaacttttt agagcaatag
    47341 gccatcagga gtatagtgat gcccgacaga agaagtgttc tgaatgaaag cgactaactt
    47401 aaggagtgag gaaagatgga aagtaatttc attgactggc atcccgctga catcattgcg
    47461 ggtttgcgca agaagggaac gtcaatggcg gcggagtctc gcagaaatgg tttgagttcc
    47521 tcaacgctgg caaacgcatt atcgcgccca tggccgaaag gagagatgat tattgcgaaa
    47581 gccctgggaa ctgacccctg ggttatctgg ccatcacgct accatgatcc gcagacccat
    47641 gagtttatcg acagaacgca gttgatgcgt agctacacta aaccgaaaaa atgagtggtc
    47701 tggcggtagc cccgcgaacg gggctgccag ctctcagacg attactcgcc tttcacacgc
    47761 tcaatatttg cacctaaagc gcgcagtttg tcttcaatgc gttcgtagcc acgatcgatg
    47821 tgataaatac gatcaaccac cgtcgtccct tccgcaatac agccagccag caccaggctt
    47881 gctgatgcac gcagatcggt tgccataacc tgtgcgccag aaagtttttc aacaccgtga
    47941 caaataacgg tattgctttc gatttcggca tgcgcgccca tacggctaag ctccggcaca
    48001 tgcataaagc ggttttcaaa gaccgtttcg gtgatgaatc cggtcccttc tgccaccagg
    48061 ttcaacagcg tgaactgggc ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt
    48121 acgttaacag ccttcggacg tttgccatgc atatccaggc taatccagtc ttcgccgact
    48181 tcgatgtccg ctccagcgtc acgcagtttc gccagcacgg cgtcgagagt atctggctgc
    48241 gcgttacggc agataatttt gccgcgagaa atcgccgccg ccaccaggaa agtaccagtt
    48301 tcgatacgat ccggtagcac gcgatagaca ccgccgccta aacgttccac accttcgatg
    48361 acgatacgat cggtgccctg accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg
    48421 gtatcgacga tttccggttc acgcgctgcg ttttcaataa tcgtggtacc ttccgccagg
    48481 gttgcagcac acatgatggt caccgttgcg ccaacgctga ctttatccat cacgatatgt
    48541 gcgcctttca gacgaccatc gacggaagct ttaacgtaac cttcttccag tttgatggtc
    48601 gcgcctaatt gttcgaggcc agaaatgtgt agatcaaccg gacgcgcacc gatcgtacaa
    48661 ccgccaggca gtgaaacttg cccctgacca aagcgcgcta ccagcggccc cagtgcccag
    48721 atagaagcac gcatggtttt aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg
    48781 gcatcaatat gcacagaacc attacgttct actttcgcac ccagctggct tagcagcttc
    48841 attgatgtat cgacgtcttt cagtttcggg acgttctgga tctctaccgg ttcttccgcc
    48901 agtagagcgg caaaaaggat aggcagagca gcatttttag cgccggaaat tgtgacttcg
    48961 ccctggagct tcgttggccc ctgaacacga aatttatcca tttagtttgt tctcagttaa
    49021 caattcatat ccgctaccgg cgaatcgccc atagctcaaa agccgttcag tttgcgatcg
    49081 cgcgcccact ccgcaggggt atacgctttg atcgacacag catgaatgcg gttatccgca
    49141 atatattcca tcagcgggcc atagaccgtc tgctgttttt taacccgact catgccgtca
    49201 aacaactcac ccacggcaat aacctgaaag tggctgccat cgccggaaac gtggacttcc
    49261 tggagggaga gagcgttcat caacacgctc tgaatttcat tattttccat gggatcttca
    49321 atcatcagtt aataaaccag cgaaacatct tagagcaaag ttgcgctggc ataaataagc
    49381 aaaaagcctc gctgataaat cagacaaggc tcgacttgca ggcaggtttg ccggacaggc
    49441 ggttaacgcc atatccggcc tgaaaaaatt taacgaggca gaacatcagc aggcaaatta
    49501 tacaatttcg cgagggtata cactttgtcg tttaccccct gaagcgtcac gttgttgccc
    49561 tgctttttcg ccagatcgat aagatggagc agcagtgcca gtccacccgt atccacgcgg
    49621 gacacacggc taagatcgat gcaggtaatc cccttcaccg cttcctcacg catttcccaa
    49681 agcggtagca gaacgtcctg atccagctct ccggataacg ccagcgtgtc acccgtctgc
    49741 atccagctca gtgactcgct cattattttt tctcttccag agtgattttc tgttgagaaa
    49801 tcgatttcag ttgcgcagtc aggccgtcga tacctttggt acgcagcagc gttccccact
    49861 cgttttgttt ggtggtgatc atactgacgc cttcagcaat catgtcgtaa gcctgccagt
    49921 tgcccgtctg ggagttttta cgccactgga agtccagacg caccggcgga cggccattcg
    49981 ggtcaataat ggtaacgcga ataggcacaa tggttttatc gcccagcggc tgttctggcg
    50041 caatctgata ggtttgaccg tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt
    50101 actcacggaa agcggcaaag taggcttcac gttgagcagg ggtcgcactc ttgtaatact
    50161 ggcccagcac cagcgcaccg gcgtatttca cctgtacgta tggcagcagt tcctgatcaa
    50221 caatggtacg cagataatcc gggttggccc gaatttgcgg ttgctcattc ttcaggcgat
    50281 cgaacgtttt ctgcgccgcc tcgtccatca gcttataagg attggtctgg tctgccgcgg
    50341 ttgccgcact cagaggtgca atcaccagca aagcgaccat cattaaacgt ttaaacatgc
    50401 gtcggttctc ctgaaattat ttcgttgtac ccacaggttc agtggtttca ttattacctg
    50461 gcgcagcagc tggcgcatcg ccactattct tattgtcatc gcctttacta ccgtaaagga
    50521 actgaccaat gagatcttcc agcaccatcg cagacttagt gtcctgaatt gtatcgccat
    50581 ccttcaggat agcagtcccc agttccgggt cttcaaaacc gacgtttaat gccagatatt
    50641 gttcccccag caggccggaa gtacgaatgc tcagcgaact ggtatctgga atgtggttat
    50701 aacgttgttc aatttccagc gttacgcgcg gcagataggt tttcgggtcc agcgtaatat
    50761 ccgccacccg acccacaaca acgccaccaa tactgaccgg agagcgcgct ttcaggccgc
    50821 caatgttatc gaacgtcgca taaagcgtgt aggtcggttc agtacgtatg gacgtcacgt
    50881 tcgccgcctt caggcaaaca aacagcgccg ccagcagtgc tgctaataaa aagataccca
    50941 cccaaatttc attttttttc gtttgcatga actcaattcc caaacatcaa tgcggtcagc
    51001 acaaaatcca gccccagaac agccagagac gagtggacaa cggtgcgagt ggttgcccgg
    51061 ctaatcccgg cagacgtcgg gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc
    51121 gtgatggcga acaccacgct cttaatcaga cagttgacca gatccatacg ccagtcgacg
    51181 gcattttgca ttgccgacca gaagaacccg ctatcaatgc ctttccaact gacgccgacc
    51241 agagatccgc cccagatccc cacggcgacg aaaataaccg tcaacagtgg taatgaaata
    51301 accccagccc agaaacgggg agaaataacc cgacgcagcg gatccaccgc catcatctcc
    51361 atactggaga gttgctctgt agcgcgcatc aggccgattt ctgcggttag cgccgaacca
    51421 gcacgcccgg caaacaacaa cgcggcaacc accggcccca gttcacgcag tagcgataac
    51481 gccaccagca tacccagact ggtttccgca ctataagtgg tcagaaccag ataaccttgc
    51541 agccccaaca ccattccgat gaacacgcca gaaaccacaa taatcagcat cgacaggacg
    51601 ccgacattat agagctggcg caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg
    51661 accagcgcat tgaataacat taacccggcc cgcccgaacg ttctcagggt tttaatccct
    51721 ttatgtccga gcgacgccag cgcatttaac agcatgagtg gcttaactcc ctggtaaaag
    51781 atcagcgtga taatcgccgg caggatagcg gaacggaaca ggcccgtcag ctatcccgtc
    51841 cagaaactga cgtacgcgcg gatcaggatt cgcctgcaac gcctgggcac tgccatgagc
    51901 gacaattttt ttgtccgcca ggatccaggc gtgatccgca atacttaaca cttccggcac
    51961 atcgtgagaa accaccacac aagtcacgcc cagcgcgctg ttcagctcag aaatcagctt
    52021 caccagtacg cccatggtga tgggatcttg cccaacaaaa ggttcatcaa acatgatgag
    52081 atccggctcc agcgcaatcg cacgcgccag cgctgcacgc cgcgccatcc caccagaaag
    52141 ttcagaaggc attagcttag ccgctccacg cagccccacg gcctccagct tcatcatcac
    52201 tgtactatgc aacaatggcg cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt
    52261 gtcaaatacg ttcatatcag tgaacaacgc cccggactga aataacatgc tcatccgttt
    52321 gcgcactgta tacaggcgcg aacgagacat cgccggaata ttctcaccat cgaaaaggat
    52381 ctcaccatga tctggtgcga tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat
    52441 gcccgatggc cccatgattg ccgtgatctt ccctcgcggc acggtcaggg aaatattatc
    52501 gaagatgcag cgattgccac gcgtaaaact gacatcgcgc atatcgacta aattcgccac
    52561 agactgctcc ataattcacc cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt
    52621 aaccttgaac ccaacatatt tacagaatat tacccgccgt ggttagcgaa agctggcatt
    52681 tgttttactt tttagccgca taaagtcaaa attaagaatc cgttacggct ttctgaaaat
    52741 cttcagcgga ccggcgagta tacctgaaga aaggacgtta gatgctttta gctacggcac
    52801 tgttaattgt tggtttactt ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt
    52861 ctattctttg ccgaaccttt ggcatcccgc cgctgatcat cggcatgacg gtggtcagta
    52921 ttggtacatc gttaccagaa atcatcgtct cgcttgccgc gtctctgcac gaacaacgcg
    52981 atttagccgt tggtacagcc ctcggctcaa acattatcaa tatattgctg atcctcggtc
    53041 tggctgcgct ggttcgtcct tttaccgtcc attctgatgt tctacgccgt gaattaccct
    53101 taatgttgtt ggtcagcgtg gtggccggtt ccgtactcta tgacggacaa cttagtcgca
    53161 gcgatggtat ctttctcctc tttctggctg tgctatggct gctgttcatt gttaaacttg
    53221 cacgtcaggc tgaacgtcag gggactgaca gcctgaccag agagcagctt gcagagctgc
    53281 cgcgtgacgg cggattgccc gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc
    53341 cagtggccac gcggatggtg gttgataacg ccacggtgct ggcgaattac tttgccatca
    53401 gcgagttgac gatggggctg acggcaattg ctatcggaac cagcctgccg gaactggcaa
    53461 ccgcaatagc gggggttcgc aaaggtgaaa acgacattgc tgtcggaaat atcattggcg
    53521 caaacatttt taatattgtc atcgtgttgg gtttacccgc gctgataacg ccaggagaga
    53581 ttgatccact ggcgtacagt cgtgactaca gcgtgatgtt gctggtgagc attatttttg
    53641 cgttgctgtg ctggcggcgc tccccgcaac cgggccgtgg tgtaggggta ttattaactg
    53701 gcggatttat cgtatggctg gcgatgttgt actggttatc gccaatactc gttgaataac
    53761 tggaaacgca ttatgtcgca cgtagagtta caaccgggtt ttgactttca gcaagcaggt
    53821 aaagaagtcc tggcgattga acgtgaatgc ctggcggagc ttgagcaata catcaatcag
    53881 aatttcacgc ttgcttgtga aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg
    53941 atggggaaat cggggcacat cgggcgcaaa atggcggcaa cgtttgccag caccggtaca
    54001 ccttcatttt tcgtccatcc tggtgaagcc gcgcatggtg atttaggcat ggttacccca
    54061 caggatgtgg tgattgctat ctctaactca ggtgaatcca gcgaaatcac ggccttaatt
    54121 ccagtgctta agcgtcttca cgtaccgtta atctgcatca ccggtcgccc ggagagcagc
    54181 atggcgcgcg ccgcagatgt gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta
    54241 gggctggcac cgaccagcag caccaccgcc acgctggtta tgggcgatgc cctcgctgtc
    54301 gcgctgttaa aagcacgcgg ctttactgct gaagattttg cgctctcaca cccaggcggc
    54361 gcactgggtc gtaaacttct gctgcgcgta aacgatatta tgcatacggg cgatgagatc
    54421 ccgcatgtta agaaaacggc cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat
    54481 cttggtatga ctgtcatttg cgatgacaat atgatgattg aaggcatctt taccgacggt
    54541 gatttacgcc gtgtcttcga tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg
    54601 atgacgccgg ggggaatacg tgtgcgccct ggcattctgg ccgttgaggc actgaactta
    54661 atgcagtccc gccatatcac ctccgtgatg gttgccgatg gcgaccattt actcggtgtg
    54721 ttacatatgc atgatttact gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa
    54781 tgagcaaagc aggtgcgtcg cttgcgacct gttacggccc tgtcagcgcc gacgttatgg
    54841 caaaagcaga gaacattcgt ctgctgatcc tcgacgttga tggcgtactg tcagatggcc
    54901 tgatttatat gggcaataac ggcgaagagc tgaaaacgtt caatgttcgt gacggttatg
    54961 gcattcgttg tgcgctcacc tctgatattg aagtcgctat cattaccggg cgaaaggcta
    55021 aactggtaga agatcgttgt gccacattgg ggatcactca cttgtatcag gggcagtcaa
    55081 acaaactgat cgcctttagc gatttgctgg aaaaactggc gattgccccg gaaaatgtgg
    55141 cttatgtcgg cgatgatctc atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg
    55201 ccgtggccga tgcgcatcca ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg
    55261 gcggtcgtgg cgcagtgcgc gaagtttgcg acttattact cctggcgcag ggcaaactgg
    55321 atgaagccaa agggcaatcg atatgagtaa agccagacgt tgggttatca ttgtgctatc
    55381 actggcggtt ctggtgatga tcggcattaa tatggccgaa aaagacgata ccgcccaggt
    55441 ggtcgtcaac aacaatgatc ccacctataa aagcgagcat acggacacgc tcgtctataa
    55501 cccagaaggg gcactaagct atcgattgat tgctcaacac gttgaatatt attccgatca
    55561 ggccgtttcg tggtttacgc agccggtact taccacgttt gataaggata aaatcccgac
    55621 atggtccgta aaagcagata aagccaagct gaccaatgac cggatgctct atttatatgg
    55681 acacgttgaa gtcaacgcac tcgtgccgga ctctcaactt cgcagaatca cgacggataa
    55741 cgcgcagatc aatctggtga cgcaggatgt tacctctgaa gacctcgtca cgttatacgg
    55801 aacaacattt aactccagcg gtctgaaaat gcgcggcaac ttacgcagca agaacgccga
    55861 gctgattgaa aaggttagaa catcctatga aattcaaaac aaacaaactc agccttaatc
    55921 ttgtgcttgc cagctcactt ctggccgcca gcattccggc atttgccgta accggagaca
    55981 ctgatcagcc gatccacatt gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg
    56041 ttacctttac cggtaatgtc atcgtcaccc agggcaccat caaaattaat gccgacaaag
    56101 tggttgttac ccgtccgggc ggcgaacaag gtaaagaagt gattgacggc tacggtaaac
    56161 cggcaacgtt ctaccagatg caggacaacg gtaaaccggt tgaaggtcac gcttcccaga
    56221 tgcactacga actggcaaaa gattttgtcg ttctgacggg taatgcttat ctgcagcagg
    56281 ttgatagcaa cattaagggc gataagatca cttacctggt aaaagagcag aaaatgcagg
    56341 ctttcagcga caaaggcaag cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca
    56401 aaaacaacaa aggccagacc ccggcacaga agaagggtaa ttaattcgtt atggcaacat
    56461 taactgcaaa gaaccttgca aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc
    56521 tgaccgtcaa ctccggggaa attgtcggtc tgctggggcc aaacggtgcc ggtaagacca
    56581 ccactttcta catggttgta ggcattgtgc cgcgcgatgc gggcaacatc attattgatg
    56641 atgacgatat cagtctgctg cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc
    56701 cacaggaagc ctccattttc cgtcgcctca gcgtttacga taacctgatg gcggtactgc
    56761 aaattcgtga cgacttgtct gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag
    56821 agtttcacat tgagcacctg cgtgacagca tggggcagtc actctccggg ggtgaacgtc
    56881 gccgtgtaga aattgcccgc gcactggctg cgaatccgaa atttattctg ctcgacgaac
    56941 cgtttgccgg ggttgacccg atctcggtta tcgacattaa acgcatcatt gagcacctgc
    57001 gcgacagcgg cctgggcgtg ctgatcactg accacaacgt gcgtgaaaca ctggcggttt
    57061 gtgaacgcgc ttatatcgtc agtcaggggc atttgatcgc ccacggcacg cctacagaaa
    57121 tcttacaaga cgaacacgtt aagcgtgtat accttgggga agacttcaga ctctgatagg
    57181 gtagaagttt gcgacgtttt agcaggagag tacgattctg aacatgaagc aaggtttgca
    57241 actcaggctt agccaacaac tggcgatgac gccacagctc caacaggcaa ttcgtctgtt
    57301 gcagttgtcg acgctggaac ttcagcagga gctacagcag gcgctggaga gtaatccgct
    57361 gcttgagcaa atcgacactc atgaagaaat cgacacccgc gaaacgcaag acagtgaaac
    57421 gctggacacc gccgacgcgc tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc
    57481 cagttgggac accatttaca ccgctggtac accatccggc accagcggtg actacattga
    57541 cgatgagctg ccggtctatc agggcgaaac gacgcagacc ttgcaggatt acctgatgtg
    57601 gcaggtcgag ctgacaccgt tttccgacac tgaccgcgct attgctacct ctatcgtcga
    57661 tgccgttgat gacaccggtt atctgactgt cccgctggaa gatattctcg aaagtatggg
    57721 cgatgaagag atcgacatcg acgaggttga agccgtcctt aagcggatcc aacggtttga
    57781 tccggtcggt gtagcggcaa aagatctgcg tgactgcctg ctaatccaac tctcccaatt
    57841 cgataaaacc acgccgtggc tggaagaggc cagactgatc attagcgatc atctcgatct
    57901 gttagccaat cacgacttcc gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct
    57961 gaaagaagcc gtcaatctga tccagtcgct cgatccgcgc cccgggcagt cgatccagac
    58021 tggcgaacct gagtatgtca ttccagatgt gctggtgcgt aagcataacg gtcactggac
    58081 ggtagaactc aacagtgaca gcattccgcg tctgcaaatc aaccagcact acgcctcgat
    58141 gtgcaataac gcgcgcaacg atgatgacag ccagtttatc cgcagcaatc tgcaggatgc
    58201 caaatggttg atcaagagtc tggaaagccg taacgatacg ctactgcgcg tgagtcgctg
    58261 tatcgttgaa cagcagcaag ccttctttga gcaaggtgaa gaatatatga aaccgatggt
    58321 actggccgat atcgcccagg ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac
    58381 gcaaaaatac ctgcatagtc cacgaggcat ttttgaactg aagtatttct tttccagtca
    58441 cgtcaatacc gagggcggcg gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa
    58501 attaatcgcg gcggaaaacc cagcgaaacc gttgagcgac agcaagttaa cctctttact
    58561 gtcggaacaa ggtatcatgg tggcacgccg cactgttgcg aagtaccgag agtctttatc
    58621 cattccgccg tcaaaccagc gtaaacagct cgtttgaccc aaccgataag gaagacacta
    58681 tgcagctcaa cattaccgga aataacgtcg agatcaccga ggcactgcgc gaatttgtta
    58741 cagctaaatt tgccaaactt gagcaatatt ttgaccgaat caaccaggtc tatgttgttc
    58801 tgaaagtgga gaaagtcacc cacacctcag atgcaacact gcatgtaaac ggtggtgaaa
    58861 ttcatgccag cgcggaaggt caggacatgt acgctgccat tgatggctta attgataagc
    58921 tggcacgcca gttgaccaaa cataaagata aactgaaaca acactaattg tccgggcaat
    58981 tagcatgtgc atggcggcct gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc
    59041 gagcctgttc cactgtttga gtgggcaggt tcttaggtga aattatgaca aataatgata
    59101 caactctaca gcttagcagt gttcttaaca gggaatgtac gcgaagccgc gtccactgtc
    59161 agagcaaaaa acgcgccctg gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc
    59221 cgcctcaggt ggtttttgaa gctatcctga cgcgggaaaa aatgggcagt accggtatcg
    59281 gcaatggtat tgccattccg catggcaaac tggaagaaga tactctgcgc gccgttggcg
    59341 ttttcgttca gctcgaagcg cctatagctt tcgatgccat cgacaaccaa ccggtggact
    59401 tactttttgc cctgctggtg ccggcagacc aaactaaaac gcacctgcat actctgtcgc
    59461 tggtggcgaa acgtctggcg gacaaaacca tctgccgccg tttacgcgca gcccagagcg
    59521 atgaagagct gtatcaaatc attacggata ccgaaggtac tccggatgaa gcgtagttat
    59581 tcggtaatgt ctcttttaga cgttgtgagg agaaacagta catggtactg atgatcgtca
    59641 gcggacgttc aggttcaggt aaatctgtcg ccctgcgtgc gctggaagat atgggttttt
    59701 actgcgtgga taaccttccc gtagtgttgt tacccgatct ggctcgaact ctggccgatc
    59761 gcgagatttc tgccgccgtc agcattgatg ttcgtaatat gccggagtca ccagaaatat
    59821 tcgaacaggc gatgagtaac ctgcctgacg ctttctcacc gcaactactg ttcctggatg
    59881 ccgaccgtaa taccttaatt cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca
    59941 gcaaaaacct gtcgctggaa agtgctatcg acaaagaaag cgatttgctg gagcctctgc
    60001 gttcgcgagc ggatctgatt gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa
    60061 tgctgcgtac ccgtctgctg ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt
    60121 tcggcttcaa acacggtatc cctatcgatg cagattacgt ctttgacgtg cgcttcttgc
    60181 cgaacccgca ctgggatccg aaactgcgtc caatgacagg tcttgataaa cctgtcgccg
    60241 cgttcctcga ccgccacaca gaagtacaca attttatcta ccagacgcga agctatcttg
    60301 aactatggtt acctatgctg gaaaccaaca accgtagcta cttgacggtc gccattggtt
    60361 gtaccggcgg gaagcaccgt tcggtgtata ttgcagagca actggcagac tacttccgct
    60421 cgcgcggtaa aaacgtccag tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt
    60481 caagcaaact gttgaaatca caaacaagct gggcatgcat gcccggcctg caatgaagct
    60541 gtttgaatta atgcagggtt ttgacgctga agtgctgtta cgtaatgacg aaggcaccga
    60601 ggccgaagcc aacagcgtta ttgcgctgct gatgttggat tctgccaaag gacggcagat
    60661 tgaagttgaa gcgaccggcc cacaggaaga ggaagcactg gccgccgtta tcgccctctt
    60721 taactctggt tttgatgaag attaatcttc atcactttga catacaactc ccttcaaact
    60781 ccccccgaca ataagaaaat cacgtactga aatcgttctc aatcaacgtc atttgtacat
    60841 tttgtgcgct tttcactttc agaagaacct taagaaaacc ttaagaggca ttgtttaggt
    60901 tttgtttaag ttaatcgacc atactggaga tcgtcagaaa atatttccag gagatggcat
    60961 gattcgctta tctgaacaaa gtcccctggg caccggaaga catcggaaat gttatgcgca
    61021 tccggaagat gcccaacgct gtatcaagat tgtctaccat cgtggcgatg gtggcgataa
    61081 agagatccgc cgtgagttaa agtactacgc gcatcttggt cgccggttaa aagactggag
    61141 tggaataccg cgctatcacg gtaccgttga gacggattgc ggaaccggtt atgtctacga
    61201 tgtgattgca gattttgacg gtaaaccctc cattacgctg accgaatttg ctgaacaatg
    61261 ccgttacgaa gaagacattg cacaactgcg ccagctactg aaacagctga agcgttattt
    61321 gcaggataac cgtatcgtga cgatgtcgct gaagccgcag aatatcctct gtcaccgcat
    61381 cagtgagtca gaggttatcc cggtcgtctg tgacaacatt ggtgaaagca cattgattcc
    61441 actggcgacc tggtcgaagt ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt
    61501 tatcgcccaa ccggcgctgg ctattgccct gcagaaagat ctgcaacctc gtgagagcaa
    61561 aacgctggcc ctcacttcgc gcgaagctta atccagctgg tgttgttgca taaacggctc
    61621 accgcctaac tgatacatct gccgtaaaat ccacgcctga cggctacgca cgtagcccga
    61681 tggcgaggag actttgaaac gaagtggatt aggtaataca gctgccagta atgcagcttc
    61741 cgaccgggta agtttgctcg cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc
    61801 aaacacaccg tcgccaaatt cggcgatatt caggtaaacc gtcaggatac gctttttgct
    61861 ccagactgtt tctatcccca gcgttaatcc agcttccagc ccttttcgta cccagctacg
    61921 cccatcccat aaaaagagat ttttggccgt ctgctgagaa atcgttgaag caccgcgaat
    61981 acggttttca ttgcgctcgt tgtgcgccag ggctttctca atggaagcga catcaaagcc
    62041 ccagtgctca ggaaatttct gatcttctgc ggcaatcacc gccagtccca tccacggcga
    62101 gatttgatcc atactgaccc agtcagaatg tgccacgtaa cgaaaattgc catgcagcca
    62161 ggcgctgacc tgtcgctcga ccattaccgc tgagaaggga acaggcgcaa cactaaacaa
    62221 cgcgatgccc ccgccccaga aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg
    62281 aacgaaacta aacaccgtta agcggctttt actcattcag ccagaaccag cacgcgggaa
    62341 accagcttat caatgccgct cgccgcttct gcaatgttct gcgccagcat atatgctggg
    62401 gtggtgacaa tcttattgtc ttcatcaacc acgatatcat cgacaggaca gggcacatgc
    62461 tccgcgccca tctcttccag cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc
    62521 agcgggaaat cgaaaatttt cggcagcatc gccggggcaa tacacataaa accaagcggt
    62581 tttccggcct gatgcatcgc ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg
    62641 ctaccaagag tggcaaaatt gcttaaattc ttcgccgcgc caaacccccc cggcacaatc
    62701 aacgcatcca gttcagcggc atcggcctgg gccagaggac ggatttcacc gcgcgttatt
    62761 cgtgccgctt caatcagcac gttgcgcgtt tccgtcatcg cttcgccagt taaatggttg
    62821 ataacatcaa cctgctgctt atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa
    62881 atagctaaca acgtcaacac cgcttcatga atttcagaac cgtcatagac gccgcatccg
    62941 ctcagaatta cgccaatttt cttcattgtg atcatccttt cgcaattgac tgaaacacat
    63001 taattttttt aataaaaatg gtacgcatca cacatttaac tgattcatgt aacaaatcat
    63061 ttaagttttg ctatcttaac tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc
    63121 atcataataa tttacggcgc agccaagatt tccctggtgt tggcgcagta ttcgcgcacc
    63181 ccggtctagc cggggtcatt ttttagtggc ttttgccacc cacgctttca gcacttctac
    63241 gtcgtgacgc cactcttctt tcatctcttc aatccattca ccgacgttat cttcccaggc
    63301 aggaaggtcc ggagactgaa tttgctgacc cagctgttgc agatggcgta accccactga
    63361 ccccgccgca cctttaattt tatgtccttc ctctacaatg ccttttttat cctgcgccgt
    63421 cagattcgac tccagcacgc tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc
    63481 gtcggtgatc agcttcggtc ctacaagttc gagatactgt tccagcatgg gaatatcgag
    63541 caatgcttct gatttactgt tctcttctgt cgtcaccgta ctctcctcat catcctgggt
    63601 atcccagaat ttcttgatca tcgcggttag cgccggaacc gaaagcggct tactcagcac
    63661 atcatccatt ccagcattga ggtactcttg tttgtctttc agcacgttag cggttaaggc
    63721 caccagcggc ggtaaatcct cgcgcggata acgcttcgtc agttcacgag agatatccag
    63781 cccggtcata tctggcaact gaatatccag caacaccagg tcgtattcgc ccggtttaaa
    63841 catctccagc gccgccttgc cggtcatggc gacatcaacg ctgttaccta atttttccag
    63901 cacagaacgc gcaacaatca cgttcagttc aatgtcttcc accagcagca cattcagcgc
    63961 cggtaaaggc atatcgtctt catcaaacgc atcatcgacc tcttctgcta ccgacggtgc
    64021 gtggatcgtc aacgtaaagg ttgaaccttt gccctgttcg ctggtaaccg taatatcgcc
    64081 gcccatattt ttcgccagac gacgagaaac ggccagacca ataccggtgc cggtggcagg
    64141 tttaccgcca tgactgtctt tcacctggta atacatggcg aaaattttat ccagctcatc
    64201 ctgcggaatg ccgataccag agtcttccac ttcaaaatgc agcatatcgc cttcatcgta
    64261 gcgcacgcgc acggtaacct ggccttgctg ggtgaatttg acggcgttac tgatgaggtt
    64321 ccacaggatc tgccgtaaac gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt
    64381 cggctccagg ttaaagcaca atcctttttg ttgcgcctgc aatgcggaga gattttccag
    64441 atcggcaagg aagctggtga aatcaaccgg ttgattatca agctggacct tgcgccgttc
    64501 catcttatcc atgtcgataa tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg
    64561 gatggtcttg agatattttt cctgctcggc ggtgagttcg gtatccagca gaatacggct
    64621 caggccgacg ataccgttca gcggtgtacg caattcgtga ctgatggtgg aaataaacgt
    64681 cgttttgtcg cggctggccc gttcaagcgc atcctgatac cgcttacgct cggtaatgtc
    64741 gcgaccaaag cccatcaaac cgtgacgttt acccacgcgg tcgtagtacg gcactttacg
    64801 gatttcaaag caggctttgc gcccgtccgg gtaatccagc cactgttcat aggtcagtga
    64861 cacattatga cggaacactt tttcatcggt ttcaatgact tttgcggcgg cttccggtga
    64921 gtaaacatca gcaggtttca ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc
    64981 catcgcgcgg ttacagccgg aaaactcttt atcttcgtta cgataaaaaa ccaggtcggg
    65041 tgaagcatca aggaaggaac gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc
    65101 ttcgcgctct ttgatttcaa ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc
    65161 tttttcacga acggcaattt cctgatttag ctgggcaata ttatctttta actgaacgtt
    65221 gaggctcaaa tcgcgctcgc gcatctcctc cagtttttgc accagccgtg acagacgttg
    65281 tcgtgactcc tccagttgct cgacgaccac cgatagaaag tagaccgccc acggcgtaat
    65341 cagcaaacca aagaagatag aacgaataac atcaatgctt tcgacctgac catgcagcac
    65401 catggttacc gccatttgta ccacaatggc aagaacgacg agggccagcg ccagcaacat
    65461 tgagaagcgc accagaccta acttcatcat caggtcaaca taatactgcg ccagcagacg
    65521 aatttgcttc attagggaat tccttcacga caacctgccc caataatacc caattctgac
    65581 agttacgtta taaattgtgt gagaaatgcg gaattcccct caacgaccta ctccgttgga
    65641 ggtatccacg gacgttccag cgccgatccc tgtacgccat gttcattcag atagcgatcc
    65701 agctcgacca ttcccgtcca tcgattttcg caccacagag gagcaagcag cgtcgggcga
    65761 cgggcgctgg cggaaatgcg gtggtagatc acttccggtg gcgtatggcg aatcatttct
    65821 ccggcagtga gcgtgtaatc ctccagttca ataccgttca aacgccccgc ttcccaggct
    65881 ttcgccataa tgctgccttt cacaatatgc agcggatgca gctttatgcc gtccacgccg
    65941 gtttcaacca ctcgctccag cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg
    66001 acaattaagt gggaacacac cttcagcccg cgctgacgtg ccagctgggt tgtacgctga
    66061 taacaggcaa aatcatggcc gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt
    66121 agccccagct ccagccacac ttcgtaaccc tggtccttat attcgcaaag cagatccagc
    66181 accgcatccg gcacgcagtc cgggcgggta ccaacacaca aaccgacaat attggcctgg
    66241 ctcaccgcct gctgatacat agaacgcagc acctgaactt ccgcaaaggt gctggtatac
    66301 gcctgaaagt aggccagata gcgtttagcg cggttaacta aattcgcctg gtgcgccagt
    66361 tgctcggcaa tggaacgatg ctgctgcgct tcatcggcaa acgaggcaac attacagaat
    66421 gtgcagccac cacgcccgat ggtaccgtca cggttagggc agctaaaacc gccgtgtagc
    66481 gtcagcttat gcaccttttg cccataacga cgggtgagat caccaccaaa catattgact
    66541 aatttctgta actgcataat ctgatagacc gcgccttgaa aagaggccaa agcctgccat
    66601 ttttagctca attcggcgat gacctggatc aatcgtcccg gcctgctttt atcaactgca
    66661 taatcaatca aaattaccga aatttcatgc ataatcacat aaatcacttt tgcttatctt
    66721 atgtcagatt tttttatctc cttatggatt ttaggcaaaa acagtagcat gaaacgtcat
    66781 taccaattaa ggcagtataa aatgctggtt ttctcgtcag ttcaaggcag gataagggtt
    66841 aacacgcctt tatgacagtc aggaattgac tgtttctcta acgacttccc ttttagcctt
    66901 aaagataaaa tccattttaa tttcagtaat ttaataaaga attttgcgct aaagcacatt
    66961 tctgtaccaa taagcttgcc atttgacctg tatcagcttt cccgataagt tggaaatccg
    67021 ctggaagctt tctggatgag cagcctgctc atcatattta tgcagtaatt gagatcccct
    67081 cttcaccgta ttaaccgatg cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat
    67141 gacacgcaaa ccccgtcgcc acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg
    67201 gttcccgcag agcctggggg aggttcacga tatgttgtac gataaatccc ttgagaggga
    67261 taactgtggt ttcggcctga tcgcccacat agaaggcgaa cctagccaca aggtagtgcg
    67321 tactgcaata cacgcactgg cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa
    67381 aaccggcgac ggttgcggct tgctgttaca aaaaccggat cgcttttttc gcatcgttgc
    67441 gcaggagcgc ggctggcgtt tagcaaaaaa ctacgccgtc gggatgctat tcctgaataa
    67501 agatcctgaa ctcgccgctg ccgcacgccg catcgttgaa gaagaactgc aacgcgaaac
    67561 cttgtcgatt gtgggctggc gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc
    67621 cctctcctct ctgccacgca ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc
    67681 gcgcgatatg gagcgccgtc tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc
    67741 cgacaaagac ttctacgtct gtagcctgtc gaatctggtg aacatctata aaggtctgtg
    67801 tatgccggcg gatctgccgc gcttttatct ggatcttgcg gacctgcgtc tggaatcggc
    67861 catctgcctg ttccaccagc gcttctccac taacaccgta ccgcgctggc cgctggcgca
    67921 gccgttccgc tacctggcgc ataacggtga aatcaacacc atcaccggta accgccaatg
    67981 ggcgcgtgcg cgtacctata aattccagac gccgcttatc cctgatctgc acgacgccgc
    68041 accgttcgtc aatgaaaccg gctctgactc cagttcgatg gataacatgc tggaactgct
    68101 gctggcaggc ggaatggata tcatccgcgc catgcgtctg ttagtgccac ccgcctggca
    68161 gaacaacccg gatatggacc cggaactgcg tgccttcttt gactttaact ccatgcatat
    68221 ggagccgtgg gatggcccgg cgggcatcgt gatgtccgac ggtcgttttg ccgcctgtaa
    68281 cctcgaccgt aacggtctgc gtccggcgcg ctacgtcatc accaaagata agctcatcac
    68341 ctgcgcctct gaagtcggta tctgggatta ccagcctgac gaagtggtcg aaaaaggccg
    68401 cgtcgggcca ggcgaactga tggttatcga cacccgcagt gggcgtattc tgcactcggc
    68461 agaaaccgat gacgatctga aaagccgcca tccatataaa gagtggatgg agaaaaacgt
    68521 ccgccgactg gtaccgtttg aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga
    68581 cgacgacacg ctcgccagct atcagaaaca gtttaactac agcgcggaag agctggactc
    68641 cgtaattcgc gtactgggcg aaaacggtca ggaagcggtc ggttcgatgg gcgacgacac
    68701 cccattcgcc gtgctctcca gtcagccgcg cattatttac gactacttcc gccagcagtt
    68761 tgcccaggtg actaacccgc caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc
    68821 caccagtatc ggtcgtgaaa tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt
    68881 aagctttaaa tcgccgattc tgctctactc cgatttcaaa cagctcacga cgatgaaaga
    68941 ggagcactac cgcgcagata cgctggatat cacctttgac gtcactaaaa ccacgctcga
    69001 agcgacagtc aaagagctgt gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgct
    69061 gctggtgctc tccgaccgga atatcgctaa agatcgcctg ccggttccag ccccgatggc
    69121 ggttggcgcg atccagaccc gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat
    69181 cgtcgaaacc gccagcgccc gcgatccgca ccacttcgcc gtgttgctgg gcttcggcgc
    69241 gacggctatt tatccatacc ttgcctatga aacgctgggc cgcctggtag acacccatgc
    69301 gattgccaaa gattatcgta ccgtgatgct caactaccgt aacggcatca acaaaggctt
    69361 gtacaaaatc atgtccaaaa tgggcatctc caccatcgcc tcttaccgct gctcgaaact
    69421 gtttgaagcg gtcggtctgc acgatgatgt agtgggcctg tgcttccagg gggcggtcag
    69481 ccgcattggt ggggcgagct ttgaagactt ccagcaggat ctgctgaacc tgtcgaaacg
    69541 tgcctggctg gcgcgtaagc ccatcagcca gggcggcctg ctgaaatacg tccacggcgg
    69601 cgaataccac gcttacaacc cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg
    69661 cgagtacagc gactatcagg aatacgcgaa gctggttaat gagcgtccgg caaccacgct
    69721 gcgcgatctg ctggcaatta cgccgggtga aaacgcggtc aacattgctg atgttgaacc
    69781 ggcaagcgaa ctgtttaaac gctttgatac cgccgcgatg tctatcggcg cgttaagccc
    69841 ggaagcccac gaggcgctgg cggaagcgat gaatagcatc ggcggtaact cgaactccgg
    69901 tgaaggcggc gaagacccgg cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt
    69961 ggcttctggt cgcttcggcg ttactccggc gtatctggtc aatgccgacg tcattcagat
    70021 taaagtcgcc cagggcgcga agccgggcga aggcggtcag ttgccgggcg acaaagtcac
    70081 tccttacatc gccaaactgc gctattcggt gcccggagtg acgctgatct ccccgccgcc
    70141 gcaccacgat atctactcta tcgaggactt agcgcagctc attttcgacc tcaagcaggt
    70201 taacccgaaa gcgatgatct ccgtgaagct ggtttccgaa ccgggcgtag gcaccatcgc
    70261 gactggcgtg gcaaaagctt atgccgattt aatcaccatc gcaggctatg acggtggcac
    70321 cggcgcaagc ccgctgtcgt cagtgaaata cgcaggttgc ccgtgggagc tggggcttgt
    70381 tgaaacccag caggcgctgg ttgccaatgg tctgcgtcat aagatccgtt tgcaggtcga
    70441 tggcggcctg aaaaccggtg ttgatatcat caaagcggcg attctcggcg cagaaagctt
    70501 cggcttcggc actggcccga tggtggcgct cggctgtaaa tatctacgta tttgccatct
    70561 gaacaactgc gcaacgggtg tagcaactca ggatgacaaa ctgcgtaaga accactatca
    70621 cggcctgccg ttcaaggtta ccaattactt tgaatttatt gcccgtgaaa cccgcgagct
    70681 gatggcacag ctgggcgtaa cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa
    70741 agaactggac ggtttcaccg ccaaacagca aaaactggcg ctgtcgaagc tgctggagac
    70801 cgccgaaccg catccaggta aggcactcta ctgcaccgaa aacaacccac cgtttgataa
    70861 cggcctgctg aacgcgcagt tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag
    70921 caaaaccttc tggttcgata ttcgcaacac cgaccgttct gtcggcgcgt cgctttcagg
    70981 ctatatcgcc cagacgcacg gcgatcaggg gctggcagcc gatcctatca aagcgtactt
    71041 caacggcacc gcaggccaga gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct
    71101 gaccggggat gccaacgact atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg
    71161 tcctccggtt ggttccgcct tccgcagcca tgaagcaagt attatcggca acacctgcct
    71221 gtatggcgcg accggtggtc gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt
    71281 gcggaactcc ggtgctatca ccgtggtaga aggcattggc gacaacggct gtgaatatat
    71341 gacgggcggt attgtctgta ttctgggtaa aaccggcgtt aacttcggtg cgggcatgac
    71401 cggcggcttc gcttacgttc tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga
    71461 actggtcgag gtcttaagcg ttgacgatct ggcgatccat gaagagcatc tgcgcggtct
    71521 tatcaccgag catgttcagc atactggctc tcagcgcggt gaagagattc tggcgaactg
    71581 gtcaaccttc gccactaaat ttgcgctggt taaaccgaag tccagtgatg taaaagcact
    71641 gctgggtcat cgtagtcgta gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc
    71701 aacaatgagt cagaatgttt atcaatttat cgacctgcag cgcgttgatc cgccaaagaa
    71761 accgctgaag atccgcaaaa ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca
    71821 ggccaaagcg caggctgacc gctgcctgtc gtgcggcaac ccatactgcg agtggaaatg
    71881 tccggtacac aactacatcc cgaactggct gaagctcgcc aacgaggggc gtatttttga
    71941 agcggcggaa ctgtcgcacc agaccaacac cctgccggaa gtttgcggac gagtctgccc
    72001 gcaagaccgt ctgtgcgaag gttcctgcac tctgaacgat gagtttggcg cggtgaccat
    72061 cggcaacatt gagcgttata tcaatgataa agcgttcgaa atgggctggc gtccggatat
    72121 gtccggcgtg aagcaaaccg gtaaaaaagt ggcgattatc ggcgcaggcc cggcaggtct
    72181 ggcgtgtgcg gatgtcctga cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc
    72241 agaaattggc gggctgctga ccttcggtat tccggccttc aagctggaaa aagaggtaat
    72301 gacgcgtcgc cgtgaaatct tcactggcat gggtattgaa ttcaaactca ataccgaagt
    72361 gggccgcgac gtgcagctgg acgatctgct gagtgattac gatgccgtct tccttggcgt
    72421 cgggacttat cagtcaatgc gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc
    72481 agcgctgccg ttccttatcg ccaacaccaa acagttaatg ggctttggtg aaacccgcga
    72541 cgaaccgttc gtcagcatgg aaggcaaacg cgtggtggtc cttggcggtg gcgacactgc
    72601 gatggactgc gtgcgtacgt ccgtgcgcca gggagcgaag cacgttacct gtgcctatcg
    72661 tcgtgatgaa gataacatgc cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg
    72721 cgtagagttc aaattcaacg tccagccact ggggattgaa gtgaacggta acggtaaagt
    72781 cagcggcgta aaaatggtac gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg
    72841 cgcggagatc gttgcaggtt ccgaacatat cgttccggca gatgcagtga tcatggcgtt
    72901 tggtttccgt ccacacaaca tggaatggct ggcaaaacac agcgtcgagc tggattcaca
    72961 aggccgcatc atcgccccgg aaggcagcga caacgccttc cagaccagca acccgaaaat
    73021 ctttgctggc ggcgatatcg tccgtggttc cgatctggtg gtaaccgcta ttgccgaagg
    73081 tcgtaaggcg gcagacggta ttatgaactg gctggaagtt taagctctgt cccagcgccg
    73141 cctcctcaga aaagagtcgg cgctgaaatt ctcctttctt tctggaaagc gcctcgcata
    73201 atctgaagaa aaccaatgta atacgctcat ctggattgtt accggtagat gcccggcgta
    73261 tagctgattc cattattcaa cgcagcgcca tataaaggga cagataatgg aatcgctctc
    73321 agagagaacc tcaacaggct accagcaaat ccacgacggt attattcatc tggtcgatag
    73381 cgcccggacg gaaacggtac gtagcgttaa cgcgctgatg accgcgacat actgggaaat
    73441 tggccgacga attgtcgaat ttgaacaagg tggcgaggcc agggctgcgt atggtgcgca
    73501 gctaatcaag cgactatcaa aggatttaag tctaaggtat aagcgtgggt tctctgcaaa
    73561 aaacttacgc caaatgaggc ttttttacct cttttttcaa catgttgaaa ttcgccagac
    73621 agtgtctggc gaattaacac cattgggaat tccgcagaca ccatctgcag aattcccatc
    73681 ggcaaagatt tggcagacgc tgtctgccaa atctttcccc ctgccctggt ccacctacgt
    73741 ccgtttactt tccgttaaaa acgctgacgc ccgcagcttt tatgaaaaag agacgctccg
    73801 ctgtggctgg tctgttcgcc agctagaacg gcaaattgcg acccaatttt atgagcgaac
    73861 actactgtca catgacaaat cagccatgct gcaacaacac gctcctaccg agacgcatat
    73921 tcttccgcag caggcgatac gcgatccctt tgtgctcgaa tttctggaat tgaaagatga
    73981 atactcagaa tccgattttg aggaggcgct gatcaaccac ctgatggatt tcatgctgga
    74041 acttggggat gattttgcct ttgttggtcg acagcgaagg ttacgcattg atgacaactg
    74101 gtttcgtgtc gatctgctgt ttttccaccg ccgtttacgc tgcctactaa tcgtcgatct
    74161 aaaagtgggc aaattcagct atagcgatgc cggacagatg aatatgtatc tcaactacgc
    74221 caaagagcac tggacgctac cggatgaaaa tccgcccatc ggtctggttc tctgtgcaga
    74281 gaaaggagtc ggagaagcac attatgctct ggcaggtttg cctaacaccg ttctggcaag
    74341 cgaatataag atgcaactac ctgatgagaa acgactcgca gatgaactcg ttcgaacaca
    74401 ggcggtgcta gaggaaggct atagacgccg ttaatttcag cggtttcgaa ttctgcagac
    74461 agtgtctgca gaatctttaa aacgaactaa gttttaagcc attaaatcag ccttaacctt
    74521 caccacaacc tttttaatct ctccaggctc gccgacaacg catcctggtt tatgcggttc
    74581 acctggcata aacacggcga acattcccgg ttttaagatg atggtttgct cgttttcaat
    74641 ggtgctgcaa agctgataat catcctcatg gtggaactct tcacactggc gcgcagtgcc
    74701 tgccatgcca aacagaatcc gttcctcacc gtttaataac agttggatat caatgtattg
    74761 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
    74821 aaagatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gcctggcggc
    74881 taatgccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
    74941 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
    75001 gcatcatggc ggtagtgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
    75061 agatacgttt ccaccagcgc cagataccct tctgccagac caacgctgcc accgaccacc
    75121 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
    75181 gagcgatgga tcagccattg cgcttgttcg tcgccctgcc cggcgcgtgt aaaaatagtt
    75241 ctcgcatccg cgcccgccag ttcaccctgc gctgccgccg caatgccgcg accagaagca
    75301 atcgcttcca cgcaacctgt gcgtccacag ccgcagactg gaccatgcgg atcggcaagc
    75361 gtatgcccga tatgccccgc cagaccgcca gggccggtaa gcagtttacc gccgctcact
    75421 acaccgccgc cgacgccagt ggaaacggtg ataaacacca tctcggttac atcgccttcc
    75481 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
    75541 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
    75601 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
    75661 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
    75721 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
    75781 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtgtggtcat agcaccgcct
    75841 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
    75901 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
    75961 gcccttcggc aatcacccga catccggcgt cgctcaacgt tttcaccagc gccagatccg
    76021 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
    76081 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
    76141 cgtgatggtg aatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
    76201 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
    76261 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
    76321 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc aatacgaatg gcgaccgcgc
    76381 ccgcctgctc tgccgctaat gccatggcgg cgacgatttc gggtttatcg agcgggctgt
    76441 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
    76501 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacagaga ccgggcaaca
    76561 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaacggca cggcaccgct
    76621 gaatggttta ccgtcgatag cgtcatgagt acgcaacgct tccgggcgca accaacgctg
    76681 aacgcgagaa ggcatatcca gcccaatcag caggatcacc acgaacgtca gactgaacga
    76741 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
    76801 ggccagtgca ccgcccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
    76861 gtcggtatcg aaataaccgc caatcagttt tggtaagatc ccggcgatcc cttgtccaag
    76921 catttgctgg aagaacagta acagaccgag cccccagacg tttgcgccgc caatcgcaaa
    76981 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
    77041 cagccagtca ccgaggaagc cacctacgca gcatcccacc gccgcgccaa agccactaaa
    77101 gaacagcaca ttggctacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
    77161 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
    77221 tacgcccgtt ggccagcgtt tgcctgtact ctgcaccata aagctgataa agattgcggc
    77281 gcataacagc ccaagaacag cgacgatcgc ggcattttgc aggttaccgg cgaagcagaa
    77341 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
    77401 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
    77461 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
    77521 aaagaacagc gcacgccagc cccagaccgg aaccaccagg ctatagacct gagcggcaac
    77581 gacggccccc acagagaagc ctgaaatcag aaaaccactg gctttgttac gcagatgttt
    77641 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
    77701 gatgaccaga cgcgcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
    77761 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
    77821 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
    77881 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
    77941 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
    78001 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
    78061 tctcccgcgg tgggcgatgc ccacacgctt tggtataaaa attgtagggt acagatgcgt
    78121 ttatttcccc tcacccggta ggggcgagcg aggggaaaca actcacccgc gctcttgcat
    78181 caactgctgg gccagcgcct tcagttctgg cagatatttt tcatctaccg gtccaaacgg
    78241 tttgcggcac agcggcacag aaacgacatc catataatgg aggacagttt tcaggccgcg
    78301 gaatacgccc gttttgatca gtaaatcaat gactttattg cattcagttt gcagtttctg
    78361 cgcggtctgg atatcgcctt ctttcagcgc cttaacgatc ccctgatagc gccagcccat
    78421 gatgttgtag gtactaccga taccaccatc agcgcccgcc agcagaccag aggcgaagat
    78481 ttcgtcgtaa ccgttataga gcacaagatc aggatgttca cgacggatct gctccatctg
    78541 atagagatcg ccagaggtct gtttcagcgc acctacgcca ggcaatgtaa caagtgtgtt
    78601 gatctgatcc agggtcagtt ttaccccact cagggctgga atgttgtaca ccaccatcgg
    78661 caaaccatcc gccgaatcaa taattgcccg atagtgatcg cagtgttctt caaagctgaa
    78721 aggatagtag aacggcgtga cggcggagac ggcatcgaag ccataacgtt tagccgatgc
    78781 cgcaagttgt tggctttcgg cggtgctgac gcaaccgacg tgggcgatga gtttaatctt
    78841 acctttcgcc tcttcggcga cgatttccag tacctgttca cgctcggaaa ggctttgtac
    78901 aaaggcctcg ccggtcgaac cacccacgta taaaccgtcg atgccctgct gaatattgaa
    78961 ctgaaccagg cgacgcagac tcgctttatc cagtgcttgt tgttggtcaa aaggagtcag
    79021 gagtgcagcc attacgccac gtaaattcgt tgccataaat acctctgaag tgatgcttgt
    79081 ctgataaacg atataccttt atacctgtta taccagatca attaagcaac accccataca
    79141 gaaagcttat aatgcgatct gcttcactaa agtggcatta tttctttttg ttggtggtct
    79201 gaccgaaagc gtgccaggta gcagagacgc tgttgagatg cgattgcaac gcacgatcgg
    79261 cttcgtcagg atcatgacgg cggatcgcat caacgatcgc aatatgctgt tgataactaa
    79321 cgttgttatg ttcgtgcagt gcctgatcgg taaccgttgg gcgtgcggca ataagccagt
    79381 cgagcagggc aacgtggatc gccatgaaga ttgggttacc ggggatctcc gccagcacgc
    79441 ggtggaaatc aacgtctgaa cgaatgaatg ccgcgttgtt atccagcgac tgactgttga
    79501 tttccagtgc ttttgccagc aaatcgattt gctcatcggt ggcatgttca gccgcatagc
    79561 gcaccaggct ggattcaaag aacagacgta attgttcgaa atgggcaatc ccaccaggat
    79621 gagaaaggaa atctttcgcc atgccggaaa gctcgccgat gatggtgtcc gcagaaggac
    79681 gtgagacgcg ggcacgttcg ccgttgttga tttgcaccag acctttgcgt tttaacgctg
    79741 ccagcgcttc gcgcacagaa ggacgcccga cgttaaagaa cgccatcagt tcgcgttcag
    79801 acggtaattg ttcaccttcg ccaaattcac gacggcggat catctgttcc agctcttctt
    79861 ccaccatttc ggagagtttt ttacgcgcca gcgggcggct acgcaagttg cgaccaattg
    79921 caggtgaaga atcttcggtt tgcgaatcaa atgcgttcat aaggcccatt ctgtaaggtc
    79981 agtgtgatta acatcatcag tgacatccta tcacaggatt gaaagtaggg gaaaatggca
    80041 ggtttttctc tttgtgcctc atcattacca taattaacgg aataattaac tattgcgaaa
    80101 aattaatgta acgcagataa aaacatcccg tttgaattat ttataagact attcacgagc
    80161 attatgaata ttatgaatgt gttcttacaa aataatcata agcgcatatt ttttaatgaa
    80221 aaatcacctc acctacaatt aaaaacacga catccgcacc ataaatagac ttgcaaaaaa
    80281 tataacatca ttgttttcaa tctgccgttt atgggattga ccgttttctt ttgacacgga
    80341 gttcaacaat gttcggcata attatatctg tcatcgtatt aattacgatg ggctatttga
    80401 tcctgaaaaa ctacaaacct caggtggtgc tggctgccgc aggtatcttc ctgatgatgt
    80461 gcggtgtctg gttagggttc ggtggtgtac tcgatcccgc caaaagcagc ggctacttga
    80521 tcgtcgatat ttataatgaa atcctgcgca tgctgtccaa ccgcattgcc ggattggggc
    80581 tgtcgattat ggcggtgggc ggttatgccc gctacatgga gcgcacagga gccagtcgcg
    80641 cgatggtgag cttgctaagc cgcccgttaa aactcattcg ctcgccgtat attattctgt
    80701 cggcaactta cgtcatcggc caaatcatgg cgcagtttat taccagcgcc tccggcctgg
    80761 gtatgttgct gatggtcacc ttatttccga cgctggtgag tctgggagta agtcgcctct
    80821 ctgcggtggc ggttatcgca accacgatgt ccattgagtg ggggattctg gaaacgaact
    80881 ccatttttgc agcccaggtc gcgggaatga aaattgccac ttacttcttc cactaccagc
    80941 ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc ccactttttc gtgcaacgcg
    81001 cttttgacaa aaaagataaa aatatcaatc acgaacaggc agagctaaaa gctctcgata
    81061 atgtcccgcc gctctattac gccattttac ctgtgatgcc gttaatcttg atgctcggct
    81121 cgctgttcct cgcccacatc gggctgatgc agtcagaact gcatctggtg gtggtgatgt
    81181 tactgagttt gacggtgacg atgtttgttg agttcttccg caagcataac ttgcgcgaaa
    81241 caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgcc aacgtggtaa
    81301 cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc actgttgatg
    81361 cggttatcag gggtgctgag cattctggtc tgggcggtat tggcgtgatg attattatgg
    81421 cgctagtcat tgccatttgt gccattgtga tgggctctgg caatgcgccg tttatgtcat
    81481 ttgccagtct tattccgaat atcgcagccg gactacatgt accagcggtt gtaatgatta
    81541 tgccgatgca ttttgccacg acgctagcgc gcgcggtttc gccgattact gcggtggtgg
    81601 tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt ggtgaagcga acagcgatcc
    81661 ccatggcagt cggtttcgtg gtgaatatga ttgccacaat cacgctattt tattaagcca
    81721 ttaaaagaca aaacaggcca ccagggcctg tttttgtatt acttcacaac gcgtaatgcc
    81781 ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag gatgagtgtc atcatcgtga
    81841 tctggcttgt cgccatcaat aaccgacata acggtttcgt tgtctgccga tgcctcttca
    81901 tcattcatga tgctggtatc ttcatcgtag gcagcttcag gctcaaacat cgtgcctgcg
    81961 ccattttcac gggcgtagat agccagcacg gcagccagcg gcacagaaac ctgacgcgga
    82021 atgccgccaa agcgcgcgtt aaagcgtacc tcatcattcg ccagttccag attgccgaca
    82081 gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg catattccat aggaacctgc
    82141 acgccaggga gcgtcacatc caccaccaag tgcggcgtga gctggttatc cagcaaccac
    82201 tcatagaatg cacgcagcag atagggacga cgtggtgtta gctgtgacaa atccatacag
    82261 attaactccg gcccagacgc atttcacgtt ctgcttcagt taaagaagca aggaaagagt
    82321 cacgctcaaa gacgcgggtc atatagcctt tcagctcttt cgcacccggg ccgctgaact
    82381 cgatgcccag ttgcggcaga cgccacagca gcggagcaag atagcaatcg accaggctga
    82441 actcatcgct caggaagtac ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt
    82501 cgcgcagttg cttacgtgcg gcatctgctt cagaagctga accgttgatg atggtgttca
    82561 tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag acggctttca ccgcgagcta
    82621 ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc atccagatat tccataatga
    82681 tgcgagattc ccacagggtc agctcacgat ccaccagggt cggaacgctc tgattcgggt
    82741 tgaggtcaat cagatcctga ggcggattgt ccttttccac gtgttcgatc tcgaaactta
    82801 cacctttctc agccagcaca atgcggacct gatggctata gatgtcagta ggaccggaaa
    82861 acagcgtcat taccgaacgt ttgttggcag cgacagccat gaaaacctcc aggtatagtc
    82921 agaattttta ctgctaccag ccaccaggtg gccagtcaga agttgtgtta cccagtaagg
    82981 aacgactctc tttgttcgaa aatcaaacaa aaaatgagca atacccgaca tttgggcaga
    83041 aaattggatg atagtttacc agattttgcg acctttgtgg tgagtcgatg ccggaaatgg
    83101 ggaaaaagag atgcgcttta gtctgaaata gttgacttag tcccttattg gcgatgtggt
    83161 ttttgttttg cctgtctgtc agacggtagc aaaaagcaac tttccaattt ttacgctgat
    83221 tcagaattta accataaaaa aacccgccga agcgggtttt ttcgcaaatt gttttctgcc
    83281 ggagcagaag ccaattaacg tttggagaac tgcggacgac gacgtgcttt acgcagaccg
    83341 actttcttac gttcaacctg acgagcgtca cgagtaacga agccagcttt acgcagttca
    83401 gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga taccgtgacg gatcgcacca
    83461 gcctgaccag agataccacc acctttaacg gtgatgtaca ggtccagttt ctcaaccatg
    83521 tcgaccagtt ccagcggctg acgaactacc atgcgagcag tttcacgacc gaagtactgt
    83581 tccagagaac gttggttgat tacgatttta ccgttgcccg gtttgatgaa aacgcgagct
    83641 gcggaacttt tgcggcgacc agtgccgtag tattgatttt cagccattgc ctataatccc
    83701 gattagatgt caagaacttg cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa
    83761 actttcagtt tacggaacat agcacgaccc agcgggcctt ttggcaacat gcctttaacc
    83821 gcgatttcaa tcacacgctc aggacggcga gcaatcatct cttcaaaggt cgcttgtttg
    83881 ataccaccga tgtggccggt gtggtgatag tacactttgt cagtacgctt gttgccggtt
    83941 acagcaactt tgtcagcgtt cagaacgatg atgtaatcac cggtatctac gtgcggagtg
    84001 tattccgctt tgtgcttacc gcgcaggcga cgagccagtt cagtagccag acggcccaga
    84061 gttttaccgg tcgcgtcaac aacataccag tcgcgtttta cggtttctgg tttagctgta
    84121 aaagttttca ttaaaagctt acccaataaa tagttacacg ttggtgaaca cccaaacgtc
    84181 ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct accccttcga atagcctatg
    84241 ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa cgtggggtcg caggattata
    84301 gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg tgacaggttt taacccgcca
    84361 aatgctcgcg cttcagatac tcttcgcttt gcatctcttg cagacgtgac aggcaacgct
    84421 ggaactcaaa cttcagccga tcgccctgat aaatttcata cagcggcact tctgcactca
    84481 ccactaattt gacatggcgc tcgtaaaact catccaccag cgcaataaag cgccgcgctt
    84541 cgctctccat caaccgcgtc ataactggta catcaaacaa catgaccgta tgaaagagac
    84601 gtgagagcgc aatatagtca tgctgactgc gggcgtcgac gcacagcgta gtaaaagaga
    84661 ccgccagcgt ctggttctcg acgcccattg tcgccaatgg ccgatggttg atttctaacg
    84721 tcggtgaatt ttctcgtttc gcccccgcca gcgccaacca tagtttatcc atctgcgccc
    84781 gggtttcatc gttgagtggc gaaagccaca gatgcgcctg agtgagtgta cgcagtcgat
    84841 aatcaacacc agcgtccacg ttcattacat cacaatgctg tttaatggca tcgattgcag
    84901 gcagaaaacg cgcacgttgc aggccatttc gataaagttc atccggcgga atatttgacg
    84961 tcgctaccag ggtaatgccg cgggcgaaca gggctttcat cagaccgcca agtagcatgg
    85021 cgtcggtaat atcagaaaca aaaaattcgt caaaacagag cacgtcagtt tcggctttaa
    85081 agcgatcggc aataatttcc agcggatcgg tctgcccctg taaggcagtt agctcttcgt
    85141 gcacccgcag cataaaacgg tgaaagtgca ggcgctgttt ccgctctccc ggcaggcttt
    85201 gatagaaaag gtccatcagc caggttttcc cgcgtcctac accgccccac atatataagc
    85261 cacgcactgg cgtatgcttt gtgtcttcgc gtttacccca cagcttaccg acccgcgcca
    85321 ttagcccact cgtcctgggg gctggtggcc tgctattgat gagttcttga taaataattt
    85381 ccaggcggct gacggcctct ttttgaacgt cgtcgggttg atggctgcct tcattgagcg
    85441 ccttcaggta ttgcgatgtt ggggtaacgc tttgcatgat cttattgtta ttccttgaat
    85501 aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc gttctacact acgcgatatg
    85561 cagtcgggat tccacttctg tggaattaac ggttatagtg gcataatcag ccgcaggcat
    85621 ggagcctgaa gccaacaccc tacggaaaca aaagacaacg ggagatgttc atgacctggg
    85681 aatatgcgct aattgggtta gtcgtcggca tcattattgg tgctgtggcc atgcgttttg
    85741 gtaatcgtaa actacgccag caacaggcgt tgcagtacga actggaaaag aataaagctg
    85801 aactggacga gtatcgcgaa gagctggtta gccactttgc ccgcagcgcg gaattactgg
    85861 ataccatggc gcacgactat cgccagctgt atcagcacat ggcgaaaagc tccagcagcc
    85921 tgctgccgga actgtctgct gaagcaaacc cgttccgtaa tcgtctggca gagtctgaag
    85981 ccagcaacga tcaggcaccg gtgcagatgc ctcgcgacta ttctgaaggt gcatccggcc
    86041 tgctgcgtac tggcgcgaag cgcgactaat ttatttttcg ggcgcagcca ttgcgccctc
    86101 ctcttctctc cctccccgac tatcatttaa tctggtgtct cattgttagc cgtctgaaaa
    86161 ttcaataaca tcaaactgtt ttgaatctct tttcttatca ttcaggtacg agagcaggaa
    86221 taatgaaaaa acaaacccag ctgttgagtg cattagcgtt aagtgtcggg ttaactctct
    86281 cggcgtcatt tcaggccgtc gcgtcgattc caggccaggt tgccgatcag gcccctctcc
    86341 ccagtctggc tccaatgctg gaaaaagtgc ttccggcagt ggtgagcgta cgggtggaag
    86401 gaacggccag tcagggacag aaaatcccgg aagaattcaa aaagtttttt ggtgatgatt
    86461 taccggatca acctgcacaa cccttcgaag gtttaggttc cggtgtcatc atcaacgcca
    86521 gtaaaggcta tgtgctgacc aacaaccatg tgattaatca ggcacagaaa atcagtattc
    86581 agctcaatga tgggcgcgag tttgatgcaa aactgattgg tagcgatgac cagagcgata
    86641 tcgccctgtt acaaattcaa aacccgagca aattaacgca aatcgctatt gccgactccg
    86701 ataaattgcg cgtcggtgat tttgccgtag cggtcggtaa cccatttggc cttgggcaaa
    86761 ccgccacctc tggcattgtt tccgcattag gccgcagcgg gttgaatctt gaaggtctgg
    86821 aaaactttat ccagacagat gcttccatta accgcggtaa ctccggcggt gcactattaa
    86881 accttaacgg tgagttaatt ggcatcaaca ctgcaatcct tgcgcctggc ggcgggagcg
    86941 tcgggattgg atttgccatc cccagtaata tggcgcgaac actggcgcag cagcttatcg
    87001 actttggtga aatcaaacgc ggtttgttag gcatcaaagg caccgagatg agtgccgata
    87061 tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt tgtcagcgaa gtgttgccag
    87121 gttctggctc ggcaaaagcg ggcgtcaaag cgggcgatat tattaccagc ctcaacggca
    87181 aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc gaccaccgag ccgggcacga
    87241 aagtgaagct tggcctgctg cgtaacggca aaccactgga agtagaagtg acgctcgata
    87301 ccagcacctc ttcgtcggcc agcgctgaaa tgatcacgcc agcgctggaa ggtgcaacgt
    87361 tgagcgatgg tcagctaaaa gatggcggca aaggtattaa aatcgatgaa gttgtcaaag
    87421 gaagcccagc tgctcaggct ggcttgcaaa aagacgatgt gatcattggc gtcaaccgcg
    87481 atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc ggcaaaaccg gccatcatcg
    87541 ccctgcaaat tgtacgcggc aatgaaagca tctatctgct gatgcgttaa tgtcgtaaac
    87601 cgggcatcag gcttacgtgt gatgtccggt taactcgtgg tatgctgctg ccgttccctt
    87661 ttttaatgac gcctccatca tgtttgtgaa gctcttacgt tccgttgcga ttggattaat
    87721 tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc agccttaacc cgctttccac
    87781 tccgcaattt gacagtaccg atgagacgcc tgccagctat aatctggcgg ttcgccgcgc
    87841 cgcgccagcg gtggttaacg tttacaaccg tggtttgaac accaactctc acaaccagct
    87901 tgagatccgc accttgggat ccggtgtaat catggatcaa cgcggttata tcatcaccaa
    87961 taaacacgtc atcaacgacg ccgatcagat catcgtcgcc ttacaggatg gtcgtgtatt
    88021 tgaagcattg ctggtgggat ctgactctct aaccgatctg gcggtactta aaattaatgc
    88081 cactggcggt ttacctacca ttccaattaa tgcacgtcgc gtaccgcaca ttggcgacgt
    88141 agtactggcg atcggtaacc cgtacaacct cgggcagacc attacccagg ggattattag
    88201 tgccacgggt cgaatcggtc tgaacccgac cgggcggcaa aacttcctac aaaccgatgc
    88261 ttccattaac cacggtaact ctggcggcgc gctggtgaac tctctgggag aactgatggg
    88321 cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa acgccggaag gtatcggctt
    88381 tgcgattcct ttccagttag caaccaaaat tatggataag ctgatccgcg atggtcgcgt
    88441 gatccgcggc tacattggta tcggcggacg cgagatcgca ccactgcacg cgcagagcgg
    88501 tggtatagat caactgcaag ggatcgtggt taatgaagtg tcaccagacg gcccagcggc
    88561 gaatgcgggt attcaggtca acgatctgat tatttcggtg gataacaaac cggccatctc
    88621 tgctctggag acgatggatc aggtggcgga aattcgccct ggttcggtga tcccggttgt
    88681 agtgatgcgt gatgataagc agttaacgct gcaggtcacc attcaggaat atccggcaac
    88741 caattaagtc gagtgctcaa aacaaaaacc ggagtctgtg ctccggtttt ttattattcg
    88801 ctaatcaatt acttattaac gaactcttcg cccagggcga tatctttctt gagcgtatcc
    88861 agcatacctt ccagcgcgtt ctgttcaaat gcgctcaggg taccgataga tttacgctct
    88921 tccacgccgt ttttacccag cagcagcggt tgagagaaga aacgggcgta ctgaccgtcg
    88981 ccttcaacgt aggcacattc gacaacgcct tgttcgccct gcagtgcacg aaccagagac
    89041 agaccaaaac gtgcagctgc ctggcccata gacagggttg cagacccgcc accggctttc
    89101 gcttcaacca cctcagtacc cgcgttctgg atacgtttgg tcagatcagc cacttcctgc
    89161 tcggtaaaac taacgccagg aacctgtgac agcagcggca gaatggtaac accagagtga
    89221 ccgccaataa ccggcacttc aacttcgcct ggctgtttgc ctttcagttc cgcaacaaag
    89281 gtgttggaac gaatgatatc cagcgtggta acgccgaaca gtttgttttt gtcataaaca
    89341 ccggcttttt tcagcacttc agcagcaatc gcaactgtgg tgttaaccgg gttagtgata
    89401 ataccaatgc acgctttcgg gcaggttttc gcaacttgct gtaccaggtt tttcacgatg
    89461 ccggcgttaa cgttaaacag gtcggaacga tccatacccg gtttacgcgc tacacctgca
    89521 gagataagaa cgacatctgc gccttccagc gccggagtcg catcttcacc agaaaaacct
    89581 ttgattttca cagcagtagg gatatggctc agatcgacag ccacaccggg agtcactgga
    89641 gcgatatcat acagagagag ttctgaacct gaaggcagtt gggtttttaa cagtagtgca
    89701 agcgcctggc caataccgcc agcagcgccg aggactgcga ctttcatcct aaactcctta
    89761 ttatattgat aaactaagat atgttgctcc gctgccgcga ccttaatcca caaaattgcc
    89821 gtttacaatg accacatctc aagaatgtgt agtcacgcaa gtttagcgtt tatgcattta
    89881 attgccgtaa tcaggaacct aacttacgta attaacagtc gcttatgcat tagcgcaaca
    89941 ttccaacagg tggtgacaat ataccctacc gttcagccaa aacaacatca atttgataac
    90001 aattaattta cttttaagca gaatttgcat gccgtgacgc aggcatgttt ctcaataacg
    90061 aaatttgata aaatcccgct ctttcataac attatttcag ccttcttcag ggctaactgt
    90121 ttgcataaaa attcatctgt atgcacaata atgttgtatc aaccaccata tcgggtgact
    90181 tatgcgaagc tcggctaagc aagaagaact agttaaagca tttaaagcat tacttaaaga
    90241 agagaaattt agctcccagg gcgaaatcgt cgccgcgttg caggagcaag gctttgacaa
    90301 tattaatcag tctaaagtct cgcggatgtt gaccaagttt ggtgctgtac gtacacgcaa
    90361 tgccaaaatg gaaatggttt actgcctgcc agctgaactg ggtgtaccaa ctacctccag
    90421 tccattgaag aatctggtgc tggatatcga ctacaacgat gcagttgtcg tgattcatac
    90481 cagccctggc gcggcgcagt taattgctcg cctgctggac tcactgggca aagcagaagg
    90541 tattctgggc accatcgctg gcgatgacac catctttacc acccctgcta acggtttcac
    90601 agtcaaagac ctgtacgaag cgattttaga gctgttcgac caggagcttt aatctctgcc
    90661 ccgtcgtttc tgacggcggg gaaaatgttg cttatccctc tcaatcccct gctttcccct
    90721 gcgattcatt taacgaatag tgcgttttac cgcgacatat cattcacgca atgaatacat
    90781 gcggtaaaaa aagcacatta tgcaaaattc attatctgat tgaaaagact ggtatttata
    90841 ataaataacc gtgtttttaa tacttttttg ttattaaaat t
//
LOCUS       DAZFYC010000007        79288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFYC010000007 DAZFYC010000000
VERSION     DAZFYC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.007"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHKW5_000614"
     CDS             complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHKW5_000614"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO7510058.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1839..3488
                     /gene="treF"
                     /locus_tag="ACHKW5_000615"
     CDS             1839..3488
                     /gene="treF"
                     /locus_tag="ACHKW5_000615"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7510059.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3539..4141)
                     /gene="yhjB"
                     /locus_tag="ACHKW5_000616"
     CDS             complement(3539..4141)
                     /gene="yhjB"
                     /locus_tag="ACHKW5_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7510060.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EISRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4661..5560
                     /gene="rcdB"
                     /locus_tag="ACHKW5_000617"
     CDS             4661..5560
                     /gene="rcdB"
                     /locus_tag="ACHKW5_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7510061.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5609..6622
                     /gene="yhjD"
                     /locus_tag="ACHKW5_000618"
     CDS             5609..6622
                     /gene="yhjD"
                     /locus_tag="ACHKW5_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO7510062.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7033..8355
                     /gene="yhjE"
                     /locus_tag="ACHKW5_000619"
     CDS             7033..8355
                     /gene="yhjE"
                     /locus_tag="ACHKW5_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510063.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8589..10649)
                     /gene="yhjG"
                     /locus_tag="ACHKW5_000620"
     CDS             complement(8589..10649)
                     /gene="yhjG"
                     /locus_tag="ACHKW5_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7510064.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10719..11486)
                     /gene="pdeH"
                     /locus_tag="ACHKW5_000621"
     CDS             complement(10719..11486)
                     /gene="pdeH"
                     /locus_tag="ACHKW5_000621"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO7510065.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11718..12647
                     /gene="kdgK"
                     /locus_tag="ACHKW5_000622"
     CDS             11718..12647
                     /gene="kdgK"
                     /locus_tag="ACHKW5_000622"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO7510066.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12743..14239)
                     /gene="yhjJ"
                     /locus_tag="ACHKW5_000623"
     CDS             complement(12743..14239)
                     /gene="yhjJ"
                     /locus_tag="ACHKW5_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7510067.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14460..15746)
                     /gene="dctA"
                     /locus_tag="ACHKW5_000624"
     CDS             complement(14460..15746)
                     /gene="dctA"
                     /locus_tag="ACHKW5_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO7510068.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15929..17917)
                     /gene="hmsP"
                     /locus_tag="ACHKW5_000625"
     CDS             complement(15929..17917)
                     /gene="hmsP"
                     /locus_tag="ACHKW5_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO7510069.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17999..21472)
                     /gene="bcsC"
                     /locus_tag="ACHKW5_000626"
     CDS             complement(17999..21472)
                     /gene="bcsC"
                     /locus_tag="ACHKW5_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO7510070.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21454..22560)
                     /gene="bcsZ"
                     /locus_tag="ACHKW5_000627"
     CDS             complement(21454..22560)
                     /gene="bcsZ"
                     /locus_tag="ACHKW5_000627"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO7510071.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22567..24906)
                     /gene="bcsB"
                     /locus_tag="ACHKW5_000628"
     CDS             complement(22567..24906)
                     /gene="bcsB"
                     /locus_tag="ACHKW5_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO7510072.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMLGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24917..27535)
                     /gene="bcsA"
                     /locus_tag="ACHKW5_000629"
     CDS             complement(24917..27535)
                     /gene="bcsA"
                     /locus_tag="ACHKW5_000629"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO7510073.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQIPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27532..28285)
                     /gene="bcsQ"
                     /locus_tag="ACHKW5_000630"
                     /pseudo
     CDS             complement(27532..28285)
                     /gene="bcsQ"
                     /locus_tag="ACHKW5_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            complement(28297..28485)
                     /gene="bcsR"
                     /locus_tag="ACHKW5_000631"
     CDS             complement(28297..28485)
                     /gene="bcsR"
                     /locus_tag="ACHKW5_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO7510074.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28758..30329
                     /gene="bcsE"
                     /locus_tag="ACHKW5_000632"
     CDS             28758..30329
                     /gene="bcsE"
                     /locus_tag="ACHKW5_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO7510075.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30326..30517
                     /gene="bcsF"
                     /locus_tag="ACHKW5_000633"
     CDS             30326..30517
                     /gene="bcsF"
                     /locus_tag="ACHKW5_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO7510076.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30514..32193
                     /gene="bcsG"
                     /locus_tag="ACHKW5_000634"
     CDS             30514..32193
                     /gene="bcsG"
                     /locus_tag="ACHKW5_000634"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO7510077.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVGWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32280..32387)
                     /locus_tag="ACHKW5_000635"
     CDS             complement(32280..32387)
                     /locus_tag="ACHKW5_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7510078.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32763..32870)
                     /locus_tag="ACHKW5_000636"
     CDS             complement(32763..32870)
                     /locus_tag="ACHKW5_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7510079.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33246..33353)
                     /locus_tag="ACHKW5_000637"
     CDS             complement(33246..33353)
                     /locus_tag="ACHKW5_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7510080.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33729..33836)
                     /locus_tag="ACHKW5_000638"
     CDS             complement(33729..33836)
                     /locus_tag="ACHKW5_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7510081.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            34312..35583
                     /gene="yhjV"
                     /locus_tag="ACHKW5_000639"
     CDS             34312..35583
                     /gene="yhjV"
                     /locus_tag="ACHKW5_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO7510082.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(35613..36617)
                     /gene="dppF"
                     /locus_tag="ACHKW5_000640"
     CDS             complement(35613..36617)
                     /gene="dppF"
                     /locus_tag="ACHKW5_000640"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO7510083.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGISFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(36614..37597)
                     /gene="dppD"
                     /locus_tag="ACHKW5_000641"
     CDS             complement(36614..37597)
                     /gene="dppD"
                     /locus_tag="ACHKW5_000641"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO7510084.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(37608..38510)
                     /gene="dppC"
                     /locus_tag="ACHKW5_000642"
     CDS             complement(37608..38510)
                     /gene="dppC"
                     /locus_tag="ACHKW5_000642"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO7510085.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(38520..39539)
                     /gene="dppB"
                     /locus_tag="ACHKW5_000643"
     CDS             complement(38520..39539)
                     /gene="dppB"
                     /locus_tag="ACHKW5_000643"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO7510086.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(39690..41297)
                     /gene="dppA"
                     /locus_tag="ACHKW5_000644"
     CDS             complement(39690..41297)
                     /gene="dppA"
                     /locus_tag="ACHKW5_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO7510087.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(42072..42314)
                     /gene="yiiF"
                     /locus_tag="ACHKW5_000645"
     CDS             complement(42072..42314)
                     /gene="yiiF"
                     /locus_tag="ACHKW5_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO7510088.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(42533..42751)
                     /gene="yiiE"
                     /locus_tag="ACHKW5_000646"
     CDS             complement(42533..42751)
                     /gene="yiiE"
                     /locus_tag="ACHKW5_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO7510089.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(43337..43627)
                     /gene="nadS"
                     /locus_tag="ACHKW5_000647"
     CDS             complement(43337..43627)
                     /gene="nadS"
                     /locus_tag="ACHKW5_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO7510090.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(43628..43939)
                     /locus_tag="ACHKW5_000648"
     CDS             complement(43628..43939)
                     /locus_tag="ACHKW5_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7510091.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            44168..45076
                     /locus_tag="ACHKW5_000649"
     CDS             44168..45076
                     /locus_tag="ACHKW5_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7510092.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(45140..46081)
                     /gene="fabY"
                     /locus_tag="ACHKW5_000650"
                     /gene_synonym="yiiD"
     CDS             complement(45140..46081)
                     /gene="fabY"
                     /locus_tag="ACHKW5_000650"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO7510093.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAIADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(46126..46563)
                     /gene="dtd"
                     /locus_tag="ACHKW5_000651"
     CDS             complement(46126..46563)
                     /gene="dtd"
                     /locus_tag="ACHKW5_000651"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO7510094.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(46560..47432)
                     /gene="yihY"
                     /locus_tag="ACHKW5_000652"
     CDS             complement(46560..47432)
                     /gene="yihY"
                     /locus_tag="ACHKW5_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO7510095.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(47426..48025)
                     /gene="yihX"
                     /locus_tag="ACHKW5_000653"
     CDS             complement(47426..48025)
                     /gene="yihX"
                     /locus_tag="ACHKW5_000653"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO7510096.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(48124..48909)
                     /gene="csqR"
                     /locus_tag="ACHKW5_000654"
     CDS             complement(48124..48909)
                     /gene="csqR"
                     /locus_tag="ACHKW5_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7510097.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(48943..49839)
                     /gene="yihV"
                     /locus_tag="ACHKW5_000655"
     CDS             complement(48943..49839)
                     /gene="yihV"
                     /locus_tag="ACHKW5_000655"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO7510098.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            50007..50903
                     /gene="yihU"
                     /locus_tag="ACHKW5_000656"
     CDS             50007..50903
                     /gene="yihU"
                     /locus_tag="ACHKW5_000656"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO7510099.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            50927..51805
                     /gene="yihT"
                     /locus_tag="ACHKW5_000657"
     CDS             50927..51805
                     /gene="yihT"
                     /locus_tag="ACHKW5_000657"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO7510100.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            51822..53063
                     /gene="yihS"
                     /locus_tag="ACHKW5_000658"
     CDS             51822..53063
                     /gene="yihS"
                     /locus_tag="ACHKW5_000658"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO7510101.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            53201..54103
                     /gene="yihR"
                     /locus_tag="ACHKW5_000659"
     CDS             53201..54103
                     /gene="yihR"
                     /locus_tag="ACHKW5_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO7510102.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            54302..56338
                     /gene="yihQ"
                     /locus_tag="ACHKW5_000660"
     CDS             54302..56338
                     /gene="yihQ"
                     /locus_tag="ACHKW5_000660"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO7510103.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILIAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            56384..57769
                     /gene="yihP"
                     /locus_tag="ACHKW5_000661"
     CDS             56384..57769
                     /gene="yihP"
                     /locus_tag="ACHKW5_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510104.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            57812..59215
                     /gene="yihO"
                     /locus_tag="ACHKW5_000662"
     CDS             57812..59215
                     /gene="yihO"
                     /locus_tag="ACHKW5_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510105.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            59283..59975
                     /gene="ompL"
                     /locus_tag="ACHKW5_000663"
     CDS             59283..59975
                     /gene="ompL"
                     /locus_tag="ACHKW5_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO7510106.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(60066..61331)
                     /gene="yihN"
                     /locus_tag="ACHKW5_000664"
     CDS             complement(60066..61331)
                     /gene="yihN"
                     /locus_tag="ACHKW5_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510107.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(61433..62413)
                     /gene="yihM"
                     /locus_tag="ACHKW5_000665"
     CDS             complement(61433..62413)
                     /gene="yihM"
                     /locus_tag="ACHKW5_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO7510108.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(62421..63131)
                     /gene="yihL"
                     /locus_tag="ACHKW5_000666"
     CDS             complement(62421..63131)
                     /gene="yihL"
                     /locus_tag="ACHKW5_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7510109.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(63348..65171)
                     /gene="typA"
                     /locus_tag="ACHKW5_000667"
     CDS             complement(63348..65171)
                     /gene="typA"
                     /locus_tag="ACHKW5_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO7510110.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            65544..66953
                     /gene="glnA"
                     /locus_tag="ACHKW5_000668"
     CDS             65544..66953
                     /gene="glnA"
                     /locus_tag="ACHKW5_000668"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO7510111.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            67127..68176
                     /gene="glnL"
                     /locus_tag="ACHKW5_000669"
     CDS             67127..68176
                     /gene="glnL"
                     /locus_tag="ACHKW5_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO7510112.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            68188..69597
                     /gene="glnG"
                     /locus_tag="ACHKW5_000670"
     CDS             68188..69597
                     /gene="glnG"
                     /locus_tag="ACHKW5_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO7510113.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            69709..69819
                     /gene="yshB"
                     /locus_tag="ACHKW5_000671"
     CDS             69709..69819
                     /gene="yshB"
                     /locus_tag="ACHKW5_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO7510114.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(70009..71382)
                     /gene="hemN"
                     /locus_tag="ACHKW5_000672"
     CDS             complement(70009..71382)
                     /gene="hemN"
                     /locus_tag="ACHKW5_000672"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO7510115.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(71571..72080)
                     /gene="yihI"
                     /locus_tag="ACHKW5_000673"
     CDS             complement(71571..72080)
                     /gene="yihI"
                     /locus_tag="ACHKW5_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO7510116.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(72199..72365)
                     /locus_tag="ACHKW5_000674"
                     /pseudo
     CDS             complement(72199..72365)
                     /locus_tag="ACHKW5_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            72662..73294
                     /gene="yihA"
                     /locus_tag="ACHKW5_000675"
     CDS             72662..73294
                     /gene="yihA"
                     /locus_tag="ACHKW5_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO7510117.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(73676..76462)
                     /gene="polA"
                     /locus_tag="ACHKW5_000676"
     CDS             complement(73676..76462)
                     /gene="polA"
                     /locus_tag="ACHKW5_000676"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO7510118.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            76826..77758
                     /gene="yihG"
                     /locus_tag="ACHKW5_000677"
     CDS             76826..77758
                     /gene="yihG"
                     /locus_tag="ACHKW5_000677"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO7510119.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(77799..79229)
                     /gene="yihF"
                     /locus_tag="ACHKW5_000678"
     CDS             complement(77799..79229)
                     /gene="yihF"
                     /locus_tag="ACHKW5_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7510120.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLEVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        18953 a        21031 c        19578 g        19726 t
ORIGIN      
        1 atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg
       61 cgtgtacacc ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg
      121 cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac
      181 gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg
      241 acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat
      301 ttccccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc cgccgagaat
      361 aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg
      421 ttgcgccgtc agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt
      481 aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg
      541 cggataaact tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat
      601 ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac ctgcctgatc
      661 ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac
      721 cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc
      781 gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa
      841 acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt
      901 ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta
      961 atattctgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac
     1021 cttacccgcc cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac
     1081 ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg
     1141 cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt
     1201 cgcgccagga atataataat aggcgggtaa ttctgccgaa ggtgtgtggc aatagtcgca
     1261 tcctttttcg cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac
     1321 atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata
     1381 gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct
     1441 tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc
     1501 tgcgagacaa agccatcata aaaaacaggg agatacgctt tttgatagga atcaatcaat
     1561 tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca
     1621 tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc
     1681 cgtattttta taaattccat ggatattagt gcgttatatt ttttgacagg ttaataaacg
     1741 tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag
     1801 cccgataatt agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa
     1861 accctaatcc agacgaactg atgatcgaag tcgatctctg ctatgagctg gacccgtatg
     1921 aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc
     1981 ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt
     2041 cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacccaaa atggacccgc
     2101 tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt
     2161 ttgttgaaaa tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc
     2221 aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg
     2281 atcacattcc gtggtcttct ctgctggcgc taccgcagtc atatattgtc ccgggcggcc
     2341 gttttagcga aacctactat tgggattcct atttcaccat gctggggctg gcggaaagtg
     2401 gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactatg
     2461 gtcacatccc caacggcaac cgcacctatt atttgagccg atcgcaacca ccggtttttg
     2521 cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc
     2581 accttaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg
     2641 cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttat tgggacgatc
     2701 gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc
     2761 gcccgcccaa cgaggtgtac cgcgatttac gcgcgggagc ggcctcaggt tgggattact
     2821 cttcccgttg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc
     2881 ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc
     2941 tgaaaggcga gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc cgtcgcgatg
     3001 cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac
     3061 gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggcatggcga
     3121 accatgaaca ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg acacctggcg
     3181 ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaat ggctgggcac
     3241 cgttacaatg gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg
     3301 aaatcgcgcg cagctggctg aaaacggtga atcagttcta tctggaacag cacaaaatga
     3361 tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt
     3421 tgcaggatgg gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg
     3481 aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc
     3541 aggaggagat atttaacatc attgccgcct gggtgcgatt tttcacttcc agacggcgat
     3601 acagggattc cagatgcgct tttaccgttc cggtactgat attcagcgct ctgctgatct
     3661 ccttatttga ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg gcgcttaacg
     3721 atttgagatc tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc agaaacatca
     3781 tccccatcgc cgcactattt accgccaacg caaatgtctc gacggttgaa tcacgaggca
     3841 caatggccag cacattaaaa tgaataactt cctgtaacca ccgtttattg caatccgtcg
     3901 ccgtgattaa caccttaacc tcaggaaact gcaccacggt tttttgcagc aaccagtagc
     3961 aaaactcacc atcctgatcg ccatcgagca taactaaggc ttcagggtaa ctttccagct
     4021 tttgccataa ctcgtctgcc tgactggccc cctgaatact cactcctgga atacgctgct
     4081 gtaaactgat tttcattcca tgaataaata ttgactgcct gtcaaacatg actatttgca
     4141 taactgaatc tccacctgaa tacgttaaaa agacttaagt agtggaaggg tattacccgc
     4201 gagaaaaaat aagaattcgc catttggcgg tggccattct acagagatga cgcgtagaaa
     4261 atagttaccg atataaatag ttacagctaa acgcctgaaa ttacatgtcg agggcactat
     4321 ttaaaacaat tttgaggatt tccttatatt ggtggttagt acgcatgcaa ttaaaaatga
     4381 aactccgcga ccacaagcaa aaataacaaa cggcaatgag acaaaaataa gcacaaatag
     4441 ccaacacgtc ctctattcac tttaaaggga atcgctgaaa aatacgctct gtttaggggg
     4501 attcaccttt ctcagaaagc tattccgccc ttttcctgct gagaaatcgc cacattcgtc
     4561 atgacaacat tgtgaaaccc ggcattagat gttagaaaaa acaataaaca atgcgatatg
     4621 cgcgttactg tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa ttcacgcaat
     4681 gcagttgttc atcaaagtcg cggagctgga aagtttttcc cgcgcggcgg atttctttgc
     4741 tttgccaaaa ggaagtgttt cgcgccagat acaggcactg gaacatcaac ttggcaccca
     4801 gcttctccag cgcaccacgc gacgggtcaa actcacgcca gaaggcatga cctattatca
     4861 acgagcaaaa gatgtgttga gtaatctcag cgaactggac ggtctgtttc aacaggatgc
     4921 caccagtatc agcggtaaat tacgcatcga catcccgcca ggaatcgcga aaagcctgtt
     4981 actgccgcgc ctgtcggaat ttctctatct gcatccggga attgagctgg aactgagtag
     5041 ccatgaccgt ccggtagata ttcttcatga tggttttgat tgcgtgatac gcactggcgc
     5101 gttaccggaa gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg tcaactgtgc
     5161 cagtccgcac tatctgacgc gctttggtta tccgcaaagc cccgatgatc tgacttcaca
     5221 cgcaatagtg cgttatacac cgcacctggg tgtacatccg ttaggttttg aggttgccag
     5281 cgttaatggc gtccagtggt ttaagtctgg cggcatgttg acggtaaaca gtagcgaaaa
     5341 ctatctcgcc gccggtcttg ccggtctggg gattattcag atcccgcgca ttgccgtgcg
     5401 cgaagccctg cgtgccgggc ggcttattga agtgttgcca ggctaccgtg ccgagccgct
     5461 ctccctttcg ctggtttacc cgcagcgtcg ggagctttcc cggcgtgtaa acctgtttat
     5521 gcagtggctg gctggcgtaa tgaaagagca cctggactga ccgactatac tttttaagaa
     5581 tgaccacgac agaaggacaa aagagcggat gacgcaggaa aacgagatca aacgtcccac
     5641 ccaggatctg gagcacgagc cgattaagca gttggataat agcgagaaag gcggcaaagt
     5701 tagccaggcg ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc aaccggttat
     5761 tgcgcacctg attcgcgcga cagaacgctt taacgatcgg ctgggtaacc agtttggtgc
     5821 ggctatcacc tatttctcat ttttatcgat gatcccgatt ttgatggtgt cgtttgccgc
     5881 agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg acaaaattct
     5941 gcaaaacatc agcgatccga cgctagccgc cacgttgaaa aacaccatca acaccgccgt
     6001 tcagcagcgt acgactgtag ggcttgtcgg cctggcggtg gcgctttatt ctggcatcaa
     6061 ctggatgggt aacctgcgtg aagcgattcg tgcccagtcg cgcgatgtct gggaacgctc
     6121 gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt cactgattgg
     6181 tttgttaatt gcgctgattg tgacgctgtc gatcacctcg gttgccggtt cggcgcagca
     6241 aatgattatt agcgccctgc acctgaacag cattgagtgg ctgaaaccga cgtggcggtt
     6301 gattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttctgga tcttctggcg
     6361 gttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc tggctgctat
     6421 tggttttgaa gtgattaaaa tcgtgatgac ctacaccttg ccatcgctga tgaaatcccc
     6481 ctctggcgca gcatttggtt ccgtgctggg gctgatggca tttttctact tcttcgcccg
     6541 tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg acccgcgaat
     6601 gccagggaaa acgcagcctt aaaataggcc ggatgcggcg taaacgcctt atccggcata
     6661 cgattttatt cggcctggct ccccgtaggc cggataagat gcgccagcat cgcatccggc
     6721 tataatgcgc acataacctc ttgaaactct tccccagagc ctcttcagcc atctatttgg
     6781 gagcaaacaa tttcattcca actcataacc ccagcatata aatccagttg gtaactttta
     6841 tttaacctga aaccagtttt atccactatt tataaaatta tgtgaagcat ttcatagaag
     6901 aaaaatcact ggcctaaaca ttatcccctt tttgcctggt ttttgaccat ttccgcgatt
     6961 tgttacacat tgaaatatca cttttgctgt gcgtaatatg gctattcgtt agccaaaaaa
     7021 taagaaaaga ttatgcaagc aacagccaca acactcgacc acgagcaaga atacacgccg
     7081 atcaactcgc gtaataaagt ccttgtcgcc tctctcattg gcacggccat tgagttcttc
     7141 gacttttaca tttacgccac tgcggccgtt attgtgtttc cgcatatctt cttcccgcag
     7201 ggcgatccaa cggcagcaac gctacagtcg ctcgccacct tcgccatcgc cttcgtcgcg
     7261 cgccccattg gctctgccgt ttttggtcat tttggcgatc gcgttgggcg taaagcgacg
     7321 ctggtcgcct cgttgctaac gatggggatt tcgaccgtgg tgattggtct gctgccgggc
     7381 tatgccacga ttggtatttt cgccccgctg ctgctggcgc tggctcgatt tggtcagggt
     7441 ctgggcttag gcggtgaatg gggcggcgcg gcgctgctgg cgactgaaaa cgccccaccg
     7501 cgcaaacgtg cactgtatgg ctccttccca cagctgggcg caccgattgg cttcttcttt
     7561 gccaacggca ctttcttgct gctttcctgg ctactgaccg acgagcagtt tatgagctgg
     7621 ggctggcgtg tgccatttat cttctcggcg gtgctggtca ttatcggcct gtatgttcgc
     7681 gtgtcgctgc atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa acaggtgaag
     7741 atcccgctgg gtacgctgct gaccaaacat gttcgcgtaa ccgtactggg tacgttcatt
     7801 atgctggcaa cctatacgct gttttacatc atgacggtct actctatgac ctttagtacc
     7861 gccgccgcgc cagttgggct tggcctgccg cgtaacgaag tgttgtggat gctgatgatg
     7921 gcagttattg gttttggcgt gatggtgcca gtcgccggat tactggcaga tgcctttggt
     7981 cgccgtaaaa gtatggtaat tatcaccacg ctgatcatcc tgttcgcgct gttcgccttt
     8041 aacccgctgc ttggttctgg caacccgatt ctggtttttg ccttcctgct gctggggtta
     8101 agtctgatgg ggctgacctt cgggccgatg ggcgcgctgt taccagagct gttcccaaca
     8161 gaagtgcgtt acaccggagc atcgttctct tacaacgtag cgtcgattct cggggcttcc
     8221 gttgcaccat atatcgcagc ctggttgcag gctaactacg ggttaggtgc ggtggggtta
     8281 tatctggcgg cgatggctgg cttgacgtta atcgccctgc tgctgaccca tgagacgcga
     8341 catcagtcgt tgtaagtatc gtgtgggcag ccaccctcac cccagccctc tccctgaaag
     8401 ggagagggag aaaagcggag cgagatgtta tatcgcgaac ataacagccc tcccccagaa
     8461 agtgaacgga aaagcggtac tcgttgtttc aacacgcaca aaacagtccc ctcgcccctt
     8521 tggggagagg gttagggtga ggggaacacg ctgctcccga actcaccagc aaacacaatc
     8581 caacctcatc acttcttcat ctgcgacaat atcgtccgac actgattagc ctccccttcg
     8641 gaaggtgaga tcagtgccag taacgccgcc gccggtgtta ccagcgttgc cagtgccgca
     8701 gcaacagcac cacgggcaat caacggcccg gctttcaccc cagcctgcgg atttttaaac
     8761 gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaatcccttt actctccgga
     8821 tcaatagtca aatccagctg ttccgaagca aaacttgccg tgccggtaac attaatcaac
     8881 gcgttctcag tatcgaaagc aaaaatctgc gggcgcgcca cgccgttggc aatattcaga
     8941 ttcgccgccg cgcagttcac ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag
     9001 ttgccgacat tcagcccaac aatctccatc aggttgcggc tcaccagccc gtcattcatc
     9061 aacagtttca ggttgccgtt actgttgcct aaaagtgctg ccaccgagtt accgctaccg
     9121 cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc cacatcgggc
     9181 atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccctg catcggcttt
     9241 ttatcgcctt ccagatgaat attcgccgca atgctgccgc ccgccatgcc aaatttcagc
     9301 ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc gctaatcggc
     9361 aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc cataacgtcc
     9421 catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg aacgcttttt
     9481 tcgcccttct tctgttcaga ccgtttcgac ttttctgccc ctttcccgga gtcaacgcca
     9541 attaacggtc ccaggtccgc cagccgcaat tgccgcgact cgacatcacc ttccagtttt
     9601 ggtcgtggct ttccggtggt gtagaccaga gaaccgtgga tatcgctatc gccaattcgc
     9661 ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc
     9721 cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga
     9781 tcacccagtg aatcgccaga aaatttaagc cgtaaatcga caccgcccat cttcattggg
     9841 tcattcacga cgccatcaaa agcaacacgg gtgttaccag agcggaaatc agcctgtacc
     9901 ggaaacggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtacccgtg
     9961 agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt
    10021 tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg cttgcctaac
    10081 ggatcaacaa aaatctccag atccgctttg cttactttgt catcaatagc gatccgccct
    10141 tgatcgaaaa gaatattatc cagccgaaac gaccatgccg acggctttgc attcgcgtct
    10201 ttgttatcat cgttggcgag attaaacgtc cagttattgt tcttttcgga gaggcgaatc
    10261 aggcgcgcgt cgggcttttc gagcttgatc cacggcagcc agaccgtttt ggtcagcagc
    10321 gccagcgggg ccagcgttgc ctctacgcgt ggcaaatgca ccatcgtgac ttcgggaata
    10381 tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg cacccagctg
    10441 cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg
    10501 aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag
    10561 tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc ccctgaaatc
    10621 gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag
    10681 tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag aaccgccgta
    10741 ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc
    10801 gagttctgaa catcacgcca ctcttccggt gtttctacgc cctcgacaat caccccgcga
    10861 caatagcgat tcattagatg taaaagctga gaaaagagtg tgcggccttc cggcgactga
    10921 cgcagcatca cgaacagctc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagc
    10981 gcagagaaat ttgccatccc ggtgccaaaa tcatccagcc acagcgggcc aaattcacac
    11041 atcgaggcaa aggttgaatc tttcggcaga cggatatgct ccaccagttc gaaacgcagc
    11101 cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta
    11161 gggccatcaa tattgaccga tgccagcagg ccgtgctcta taaagaagtc ggctttttgc
    11221 gccagcaaat caatctgctc tttcacaacc tccatccgat ggctgacggt gatttcagta
    11281 aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac caccgttaat
    11341 agctccacgg ccattaaccg tccgcatgtt tgatagatcg gctgccaggt gtaagcacgc
    11401 tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt
    11461 attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc
    11521 ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggc cggggtaaat
    11581 ttcctcactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa ataatggaac
    11641 actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac
    11701 agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc
    11761 tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg
    11821 tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta acggcgctgg
    11881 gaacggacag ttttagccag caaatgctgg acgcctggca cggcgagaac gttgatactt
    11941 ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca
    12001 ccggcgagcg tacgttctac tactggcgga acgaagccgc cgccaaattc tggctggaga
    12061 gtgagcagtc tgcggcgatt tgcgaagagc tggcgaattt cgattatctc tacctgagcg
    12121 ggattagcct ggcgatctta agcccgacca gccgcgaaaa gctgctttcc ctgctgcgcg
    12181 aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg
    12241 ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg gatatcgcct
    12301 tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg
    12361 cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc
    12421 tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtgaaactg ccgaaagaaa
    12481 aagtgatcga taccaccgca gctggcgact ctttcagcgc cggttatctg gcggtacgtt
    12541 tgacgggcgg cagtgcggaa aacgcggcga aacgcgggca cctgaccgca agtaccgtta
    12601 ttcagtatcg cggcgcgatt atcccgcgtg aggcgatgcc agcgtaaacg cagcatatgc
    12661 aaaaggctgg ataaagtgtt actcctctat ccagctcatc cataaatacc tgatggttgt
    12721 gctaactcat caccatcagg cattactgtg caggtggaat atccgtcact tcaggatgta
    12781 cgtcatccgt ggcgacagag gtggcggcag cagcagtcga tggggccatg atttgatccc
    12841 aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc agcagtatta
    12901 acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc accgtcaggc
    12961 tgttaaggaa atcctgccgc agtttctgat actgctccgg cgcgatatcg acgacctgat
    13021 tttgcaacga acgcatccgc tgacccatca gaatatcggt atcagcccgc gcataggcgg
    13081 caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac tcttcttccg
    13141 gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg ttgctgttca
    13201 gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac agcacacggc
    13261 agtcaaatcc aagaccgatg tctttgctgt tactggcgct taacgcttgc tgaacatgcc
    13321 agaacagcgc ctcgcgggcc aggtccgcac gccagtagcg cagcagtgcg gctgattcac
    13381 gaatcggctg ccacggcgta tcccacatga tagataaccg gtcctgacgc accgcgtcag
    13441 tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga gctggcgttt
    13501 cacgtttgcc tttcagttcg ccaaacgttt tgtttatttg gtcgacaacc gagcgcgcat
    13561 ccacgtttcc caccaccagt agcgtcattg catccggggt gtaccatttc tgatagaaat
    13621 ctttaatttt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg tgacctaaca
    13681 aggttgaccc tttcagacga tagcgccacc agccctcttt agtatcggca ggccaggttg
    13741 ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggtgtgatg gtcaatttgc
    13801 cagtggcatt tgccagataa gagagcgctt ctttcagcaa atcgttacgg ttattgggca
    13861 aactcagatt aaacagcgtg gtgtcataag agacaattac cggcggcatc gggcgtttag
    13921 ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc gtcaaggcca ccgctttgcg
    13981 ttagcgcaat acgagggatg gcgtgactgt aaccgctctg ttgtgtactt tcggcgagcg
    14041 aaccggtatt aaccagcagg cgaatttcaa cacgatcgct gggacgctgg ggggtggtca
    14101 gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga tcaggctgga
    14161 gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc
    14221 gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa aaaaactgcc
    14281 cgttgtcggt cagtctaaat gacgttaaaa acacttcgtg ttagaccgca agaacatgaa
    14341 aatgtcacgg aagaagtgaa ataaacctga actcacccag gtggtaagtt caggcacagg
    14401 ggtcaattat gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa gtgatgagat
    14461 taagaggata attcgtgcgt tttgccatcc ggcgcacgat tattcagcac atcgtccagt
    14521 tttttgtggt ccagttcttt cacccactta gcaacgacaa tggtcgctac gccgttaccg
    14581 accaggttag tcagcgcacg agcttctgac ataaagcggt cgataccgag gatcagcgcc
    14641 agacccgcta ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac gataaagcca
    14701 ctacccgtta cccctgccgc ccctttagaa gaaagcagca acacgattaa cagcgtgatt
    14761 tggtggacga tatccatctg actgttagtg gcctgggcga taaacaccgc cgccattgtc
    14821 aggtatatcg atgtgccatc aaggttaaac gagtagcctg tcgggatgac cagccccacc
    14881 accgatttgc ggcagccgag tttctccatc ttgtcgagca tacgcggcag cgccgactcg
    14941 gaagatgaag tccccagtac aatcagcagt tcttcacgga tgtagcggat aaatttgaag
    15001 atactgaaac cagtcgcttt agcgattgaa cccaatacca gcaccacaaa caggatacag
    15061 gtaatgtaga aacagataat cagctgcccc agttgcacca gtgtgccgac gccgtattta
    15121 ccgatggtaa acgccattgc cccgaacgca ccaataggcg ccagacgcat gatcatattg
    15181 atgatgccga agatgacctg cgagaaactt tcgatgacgt taaaaatcag ttggcctttg
    15241 ctgcccagac ggtggagcgc aaaaccaaac agtacggcaa acagcagcac ctgcagaatg
    15301 ttaccgctgg caaatgcgcc aatgacgctc gccgggatga catccataat gaaggcgaca
    15361 atgccctggt ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc aagcgttgcc
    15421 ggatcgacgt tcattccggc accaggctgc acgacgttaa cgatgataag accaataatc
    15481 agcgcgatgg tactgacaat ttcaaagtaa agcagtgcga ctgcgccggt acgaccgacc
    15541 gccttcatgc tttccatgcc cgcaatgccc gttacgacgg tacaaaagat gacaggagcg
    15601 atgatcatct taatgagctt aacgaagccg tcgccaagcg gtttcatttg ctcgcctatt
    15661 tcaggataga aatggccaag gagaatacca atggctatcg ctgtcaggac ctgaaagtaa
    15721 aggcttttaa acagagaggt tttcataggg tgtcctttag taaaaccaca ggtcttgtaa
    15781 ggttatgggg tacctgcggc cttaaaataa cacccagaca acatcacaga aatgtacctg
    15841 gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa ccagcacttt
    15901 ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc tcttcgaaga
    15961 tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg cccgcttttg
    16021 ccagccagtc gcgttgtgct tcagtctcca cgccttcggc aatcatttgt aagttcaggc
    16081 tctgcgccag catgataatt gcagcaatca tgctgctatc ttccggcaag ccttcaacaa
    16141 acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc agctgacgca
    16201 gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca ttgcgcagcg
    16261 gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct gtcacttcca
    16321 gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata tccgccacca
    16381 tattcgggtg catcagttgc agcgcagaga ggtttaccga caagggcagc ataatgccgc
    16441 gctcttgcca ggctgcaagc aatcgacagg actcttccag cacccagtga ccgacggtaa
    16501 ccatgagccc acagcactca atgcgatcga ttaagccatc cggcaggtcc caactgccat
    16561 ccggttgctg gatacgcagt aacacttccg cactgaccag tttaccgctg gtcatctcga
    16621 cctgtggctg taaccaaatt gcaaactgat gattttccag tgcattaagg atatcgctct
    16681 cttccgtcaa ccgcttctgg gcggcttcca tctgctgcgg atcaaagaac tgaatctgat
    16741 tcttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa agctgttcgg
    16801 cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg agttgaatac
    16861 gttcaatcgg caggcgctcg ctcatgatag tgagcacttg ctgacctaag gtgattgcgt
    16921 gccacggttc ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta atctgcgcga
    16981 ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc agcagaattt
    17041 ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag gtgatgatca
    17101 tcagcgcggt ggtttgttta cgcgcgacaa cctgctccag catctccatc agcaaggctt
    17161 tgttcggcaa atccgacacc gggaagcgca tcgcattttc gttctgttct tcataatggc
    17221 gctgcagcaa ttgctggttg aggttgtaac tgcgcaccaa cataccgatt tcatcgtcct
    17281 gatgcagacg cggtaatgcc agttggtgac caacaagctc ctgggctggg atggcgttaa
    17341 gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac cagctgatgg
    17401 cgacggtcag gataagcgac aaaagtaagt aaatggtcac taacgttgag agggtgctca
    17461 tcacgaactt atacatacgg aaggaatccg cctgtagtac cagataggca attggctgcg
    17521 ggtttgccgg acgttcgagc gagtaaacgc ccagcgagat ttgaaccggt agctcaaaca
    17581 ggcgagtaac cattaccggc accgggcgct ctggaataaa acttttacgc agcgcctgga
    17641 actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta atgctggcaa
    17701 gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag ggttcgcgga
    17761 cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgt tggaccagat
    17821 ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct gccaccattg
    17881 ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc ggaaatccca
    17941 gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat ctgaaaactt
    18001 accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc tgccatccgg
    18061 cggcggaata acgtacgtag agcaggaaat ggctgggtgc gtaatctttc gcctgctgga
    18121 tatcaattgc cgtgccaaca aaccagttgg aagtaacacg tcgttcaagt aatgcccgcg
    18181 ccgtgtagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca gcttcttcct
    18241 gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg gtgcgtgaat
    18301 gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc tcccgccaca
    18361 tcaccggtat ggcaaacgac aggtattcct gcggactgta gtaaccgccc tgaccgagtg
    18421 agtagccact cagatctttg tcgtaatgcc agatcatgtt gttcaggccg attgtgacgc
    18481 ggcgattgtt ctggttaatg accttatagt aatagcccgt catccagcgc acgcgccagt
    18541 tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag acgccgtttg
    18601 cttcaccttt atcgtagctc agacttagcc ccacaccgtc ggcacgtacg ccaccccatt
    18661 ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa gaactggaga
    18721 tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg ctgtaactga
    18781 tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtaccgata tcccagctcc
    18841 agacgtcatt tcgccagccg accgccacgc tggcaccgga atccgactgg ctgcggttgc
    18901 cgctacagtc ctgtaatgta caggtgcccc agttgtcatc ccatttgcca tcggcattag
    18961 tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc cgcccgtcag
    19021 aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag taaccaccgg
    19081 tgccgctcga accccagtaa tcgtgctcaa gggtgacgtt aagatcctgc tggcgataga
    19141 ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg tcgttacggg
    19201 tcagtcgggt gaatgtgtcg ttatcctgcg gacgcgtcgt agtcacaccg gatgcgacca
    19261 tggcgtcttt gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct tcaaacttcg
    19321 caccgtcgcg cagcaccatc gcgctttcca tcgacggtgg ctgagatttt gcctgcggga
    19381 tcaacttatt aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc tgcgccagcg
    19441 ccacgcgccg ctgtgtgttc agcgaggcgt tatcggtagc gggcagtttc gccagctggc
    19501 tacgtgccgc cgctgtgtca ccggcagcaa tatccacttc cgtcagcccc agaatggcat
    19561 cggcgttagt tggctcccgc gtcaggacat tctgatatgc agcgcgggcg gcggtgtaat
    19621 cacgtcgctg ctgcgcccag tcagccagcg tgaggtcaat acgcgtggaa ggtggttgct
    19681 gacgcagcat cgcttccgct tcagcttctt tgccgctttc tcgcaggcga ttagcagttt
    19741 ccagcacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg ttccactgcg
    19801 cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggtcatga ccagagaggt
    19861 acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc agattgcgca
    19921 ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgcgaa agtcggtaag
    19981 taatccatac gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct gccgcctgcg
    20041 cccatttgcc ctggttttcc agtgcctctg cctgctgtgc cagacggtcg ttttgcaggc
    20101 tgcgttcgat atcatcaatg ctacgccgct gactggcaga gagcgaggcg ataaacgctt
    20161 cagctttttc tggcgattgc tggcggtaaa tatttgccag cccgcgcacg gcgttagtgt
    20221 tgccgctgtc catacgcaag gtctgctgat aataacgttc ggcggcggga taatcttttc
    20281 gcgccatcgc cacatcgccc agccccagca ctgcataact gtcggtgtta tcgacattac
    20341 gcgcctgctg gaacaggcgt tctgcccggt caggattatt ggctttcagc gcagcatcgc
    20401 cctgctggat cgccagccag tagcggttta ctttcagcag actgttccat ttgtcgttgt
    20461 tgctgctgtg cgggtccagt gcgagggctt tttccagatt cgccactgca ttggcgcgat
    20521 cgcctttctg agaatacgcc tggcccagcg cccccagagc ttcactgtct ttcgggttcg
    20581 cccgcaccgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag tccaccgccg
    20641 ctaaaccttg cgcacgagcg cggaaagcag gatcggccag ctgtttttgc tgttctgcca
    20701 gttgcgattg cgcagccgcc acgctatcgc catcactaaa gatcgagaga tattttttca
    20761 gcgccgacac actggcatca ctgacgggca tgtctttaat ctgcccgtac cagattttag
    20821 aggccccttc gcgcccggcg ttcgattttg ccatctgttc caggacggca aaaccttcgt
    20881 cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc gtattgcccg
    20941 gtgcatccgc attgatgcgt tttaactgat taatcgcttc gccacggcga gccggaattt
    21001 tcgccaccgt actccagtac tcgacagcaa tgtcaccttc cggcggcgca ccgttgaaca
    21061 gtttgttgta actcgccaca gcttcttctg catgaccggt cgtcgcctgc aatcgtgcct
    21121 gttgcagtgc ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc gacgatttat
    21181 acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc gcgccatcaa
    21241 tatcgccctg acgtaacaaa gaacggaaac gggcggcaac gacgtccggg ttattcggat
    21301 caataagttc cagccgatat aacgactgtt gcaccagatc ttcacgatgg gtcgcttcgc
    21361 ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg accatcggca
    21421 tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc attcctggcc
    21481 ccagtcaggt aataactcac cttttgtcga gaagcggaaa cggtgttgat cccagccttg
    21541 tccaaacagg gtcagcacat agttgtaata ggcatcgctg ccgggaaagt tatcggccac
    21601 gcgctggcgc tgaacggcct gcgcatcgcg gttttgtaaa aagggcagca tggcggcaga
    21661 aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt tttccggcgg
    21721 ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca tccgcgcttt
    21781 ttgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag cgtcgtagct
    21841 gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat agcgcaccca
    21901 gtctggcgaa aaacctttcg gggcggtttc cagcaataaa cgttgattgg tttcgcgcag
    21961 cgtggtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg gcaggtagct
    22021 ggggttaaaa cgccagctgt tatcctcagc aaaacccact ttgcccggta acaacatgga
    22081 acccagccca ggcaccgtca ccacttcctc ccgcgcgata cgttttagca acgcgctgcc
    22141 gatgtcggta taacgctgct ctttccacaa acgccccgcc tccagcaacg accaggccat
    22201 ccagacatca ccatcggagg ccgaattgct gtccagcact tcccacttac tgttctcttt
    22261 cttgccccac agccaggcgg gcaaacgttc ttttaaagag ccctgagcga gattgttctg
    22321 cgtccagtcg agaatattat cgaaagctac acggtcgtta gccgccaggg caaagaacat
    22381 accgtaactt tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt cgatgacgcg
    22441 cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggccagg tacaggctgc
    22501 ctgaacacta aaggcagcca acagcagcat cgtcacgatt ccactacgca acacattcat
    22561 cttcaattac tcgttatccg ggttaagacg acgacgacta ataattcgca gcagacgcca
    22621 cagtacccat gccagcaata tcacactgat agccgccagc accgccagca gaatcggatg
    22681 gtttgccagc gcataccaca agcgctcgaa ccacggcaga tgacctacgt aataaacgtc
    22741 gccaacacgt aggctgttga taccggactc gcggatcacc gcgaccgaac cgaacatggt
    22801 ggcgcgtttg ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc gtgggctatc
    22861 tgccaacagc gcaatcacgc tgcgctggtc gttatacggc gactggaagc caatcaccgc
    22921 cgccatcgca ccggaagagg tcagcgttga ccgggtttct gccgcgcgat cgctctcgtc
    22981 cggcacaatg ccggggaacg gggtctggcg catcggtgtt ttcacccagc tttcggtcgc
    23041 ctgcaccaat aggtcgatct gcttatcgtc tttcagtttg tccgggatac caccgatgat
    23101 catgatgtcg gcatctttgc cctgaatggt gctgccatca tcggtcaccg tcagattaat
    23161 cgccgggaag cccgtctgtg cgccgataaa accaacagta ttcagcaacg tttccatctg
    23221 tgcttcgtta ggcgctttcg gcatcacggt gatggtttgc gacagatccg ccatccggct
    23281 gaatgggaag cccgcgttag caaaggcgcg tagatccggc atcgggatga agtggtaata
    23341 cttcgagaag tcgatggtgg agtcgtcacc aatcaccaca tgattctgca ccggctggaa
    23401 ggtaatacag ttatccaccg aaccgcccgg catcgggttc atatactcaa agtcgaagcg
    23461 cagctggttg gtcgcgccca gtttcagcgc cggaatagag acatctgttt tgccatccag
    23521 caaaccttgt aataccggaa tccgcagcag caggcggttc gcctcctgtt tgctgctcag
    23581 gttgaaggat tgcaggaact ggttattcag gctgatatcc atccgcgaac tgtctttcac
    23641 cggcggcatg gtgtagcggt aattaatatc catatcaatg ccggtactgc gcatcaggta
    23701 gagatccggc ggcaggttta gcgaaacgtt aatcgctgct ggctcaagac cgctggattg
    23761 taactgttct tcataggttt tcagttcgcc aaatgtgacc ggacgatcgg tacgtaccca
    23821 gttcggcgca tcgtacggct tacgcggtag cagcggtttc acttcattca ctaccacgct
    23881 ttcaccacgg aacagaatgt taccctgagc gatacctttc gctgcctgca acaggtcttt
    23941 gtcgtcacga ccaaacacca ccagcagttt gacgtaagga ttctgcggat ggttaatcat
    24001 ctcaatcaca ggggctttta ccgccggatg atcgcgcagg aagtccggcc gtttgtcgtt
    24061 ggttgcaaag acaatggcat tgcgatccgg cagttggtta tagagtaccg ggaagttctg
    24121 cccacgccag ccagaacgcg aaccaaacca cgaggcgaca atggcagagg cttgttgcag
    24181 cccaacatcc ggcgcacccg caaagaccat cggcaaggtg ttggtgcggt tatcacgcgg
    24241 atcaaagaat ggcaccggga agtgtgacag gtcattcttc acattcaggg tctgataggt
    24301 cagatccagt ccactgctcc gcccaacatc cagccaaagc gtggtgctgg ccgggttttc
    24361 gcacacgtcc tgataatggc cgacaaactc cagccgcaca cggttgaagt cggtaataaa
    24421 cagtgggtta atgggcattt gcgccagcgt ttttttaccc aactgttctt tggtcactgg
    24481 cagcacgccc atcagctcat cattgagata aacctttaac tgcgactgga caggcagtaa
    24541 cgatggcgat ggcgtgtatt cgaggttgag catcgccttc gtcaccactt catcgctgcg
    24601 cataccaaac tcaatgctgc cgttcgggtt aatgccacgt agcaccatgc tgcccggcgg
    24661 cggcgcaatt tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct gcgccacgac
    24721 tggcgcatca gcgccctgca cgcccagcat cacttgcccc acctgcggat tttgttccgt
    24781 ctgggcggct acagctggct cagcattgat cagtggttgc gttgctggcg tcgcctgcgt
    24841 catgaaagag gggaacgcac tcatccccat agccactgca caaatccaga atagttttct
    24901 tttcatcgcg ttatcatcat tgttgagcca aagcctgatc cgatggttgt gccgtttcgc
    24961 tccgctccgg gcggcgcgga ataaacgata caacccagga aaccagagaa gtcagcacac
    25021 ggaatatgcc cttcaccgaa gaaggcgcaa actccgccag atggcggtag ccacggaagc
    25081 cgagcttcag aatatccagc agactttcca gcggcttatc ttccgggtag ctgtcctgcc
    25141 agagcgccca tgtatccgca cgggcaaacg tacactgcac aaaatcgata tgttgctggg
    25201 tggtgagcgg cattaatttc agcccaactt cattacccat cacgcgcgcc acctgggtcg
    25261 ggaagacgta ttcctgctga ccgcgtttaa gcaacagatt cactttctgc ccttccagaa
    25321 tctgcgcctg accgttgatc ttgatcccca aaccaccgtc ggagaaatcc tgaacggtac
    25381 acgagaagag gtgaccatct tcgcgggcaa ttgccgcggg catcgtcatc tccacgcggt
    25441 gcgatcggcg tacctgtttg ctttctaccg ataccgcaac tgcgccgcca agaacaatca
    25501 ggttgtagaa cacccacacc atactgacga ccacggtgag catctcggtt ggcgggccat
    25561 agaagtagcg ccagatgcct accgcaacgc ccaccaggtt gagtagaaca aggaagatgt
    25621 agggccgcga gatcacccag tcgacgtact cttcttccac cagtccacct ttggcggtga
    25681 cgttaaattt gcctttgtgc gggttaatca gcgccaccag cgtcggtggt gcgatatacc
    25741 acgccagcac cgtttcgtag atttcactcc agaaagagtg gcgatatttg ccctggatct
    25801 tcgagttggt caggctggca tggatcatat gcggcagcac gaacagggcg atcatcaacg
    25861 ctggcgcata gatgatgtag gcatgaagca gcaggaacgc cagcggcgca gtcaggaaga
    25921 tcagtcgtgg aatgcccgac aagaagtgga acatggcgtt gacgtagcac agccgctggg
    25981 cgaatttcag ccctttaccg gtaagcgggt tatcgagacg gaagatttgc accatcccgc
    26041 gtgcccagcg aatacgctga ccgatatgcg ccgacagact ttcggtcgcc agccccgccg
    26101 cctgcggaat acgcatatat gcagaggtgt agccacgacg gtgcaggcgc agagacgtgt
    26161 gcgcatcttc ggttaccgtt tcgacagcaa taccgccaat ttcatccaat ggtttgcgac
    26221 gaattactgc acaggaaccg cagaagaaag tggcgtccca catatcgttg ccatcctgca
    26281 ccagaccata gaacagcgtg ccttcgttcg gtgttttacg gaaacgcccc aggttgcgtt
    26341 caaacgggtc cggcgagaag aagtggtgtg gaatctgcat catcgccagc tgtttctctt
    26401 tcaggaacca gcccatggtc atttgcagga acgatcgcgt tggtacgtgg tcgcagtcga
    26461 aaatcgacac gaactcgcct ttggcatatt tcagcgcatt gttgatgttg cccgctttcg
    26521 catgttcatg agtggtgcgg gcgatatatt tcacccccac gttttgcgca aactggcgaa
    26581 actcttccct gccgccgtca tcgaggatcc agatgttcag tttgtctttc ggccagtcga
    26641 tacccagcga ggcgtaaatg gtatttttca ccacattgag atcttcgttg taagtcggga
    26701 caaagatatc caccgacggc cacagcgaca tatccttcgg caatggcacc ggctgacgat
    26761 tcagcggcca tactacctgg aagtagccga gcaccagcac aatccacgcg tacgtttcag
    26821 cgaagagcag aataagcccg cacaccaggc tgaccggatc gtcccagttc agcgtagagg
    26881 tgtaacgcca ccagatataa cggcaagaaa cggtcagcga cagcacaatc aacattagcg
    26941 ccgagaagcg ccccggcatc cgccgtacga tcagcgctac accccacagc agcatcagga
    27001 agataaactg cgccagcggg ttaaacggtt gagtaacgca gattaacgcc agaatcagcg
    27061 agaaggtgac gatgataccg aggatcaacc gccgcgcccc ggcactcaaa tgaccgagct
    27121 cttttttctc atcaagatgc tgtgttttat ggctaacgcg ctcaggcagc tcgttcatcc
    27181 attgatggta acgtccacga atattctgca gacctgaaaa tgcccgcctg cgcggtttcg
    27241 gcgtttcttt gcgcgatgta ccgatcagta accagcatgt ttgaatgaga taacggaccg
    27301 ggtccagcgg acgcggacgc gaggcgttga tatgcggata caggttttta tgttctgcgc
    27361 gaatacgctg ccagcgcggg tgctccagcg gaataaaaat ccaggccagg atcatccaga
    27421 aacagccgag cgtcgcgctg aaagccgacg caccgtgacg acgataatcg cgataacgcc
    27481 cgataagccg cgcgttgacc ggcgggataa gcaaccaccg ggtcaggata ctcatgatgc
    27541 actcccgact ggcgttttca gcccggagta gttcaacagg caccagttcg ccagcgtcag
    27601 tatctcttca gccgccagcg catcactgcg atattcacct actggttgct tagccgccag
    27661 gcattcagcc atcgcttcat cacgatgaat gagcatcggc agtaatcggc gctggctttg
    27721 caaccaaagc tggtaaatat cgtcctgaac ctgactgcca atacggaagt tattaatcaa
    27781 aatatgtgcg ccatccggca gcgcttgctg atgcagtcgg atatggcagt tggcatccac
    27841 attgacgatt gccagcgagt gatcgcacaa actcagcagc tggtgggtta tctgcgaggc
    27901 atcacgcggt aagtcgatta aaatccactg gtaacgcccg ctggctttta gttgctgtaa
    27961 gccggagcaa atatcgctca gccgggtttg ccagtgctgt ggattttctt gttcttcaat
    28021 ggataactga ccaaaaggca gcaaatcgag ctgcgaggtg tagcgcaacc cagcgtcacg
    28081 ccagtcctgg ccaatccagc atcgctctgg cccagccctg acggtgggta aaatcaacat
    28141 taaatgacag gcgcaacaag ttgtccgggc aggcatcgac caccagaaca ttttctccca
    28201 acatttgtaa tgaccaggct aatgcggcgg tgatggttgt tgtccccacg cctccccgca
    28261 ccccctgcaa tcccagtacg gccattcacc aatcccctac ttttgttgcg caaactctgc
    28321 cagcaacggc cagcgtttta atgccgcggc caactgttcg cgttgggaaa tatcggcata
    28381 atcaatatca ggcagtgaaa atgcctgctt taacgccaca atatcattct ggaagatata
    28441 gcctatcgcg ggatcaggca gagtatctgg ttcattgtta ttcattttgc ttgatcccta
    28501 tgaaataacc gtaccgttca tgagacaatt ctaaccgtga gcatttcgcc ggtttattgt
    28561 ttacggcgag acgggaattt gctcactttt tgactttccg ttacgcaaca gaaaaagtta
    28621 aaaacgaaaa gactaaaatc acacgtcttg tgaccttcta aattgattca catgctaaat
    28681 ctgataagtt ttaatttcaa tggtaggttt atttcttagc tttcgctagt aaactgataa
    28741 tcagacaaaa tggtgacatg agggacattg tggaccctgt attctctatc ggtatctcat
    28801 cattatggga tgagctgcga catatgccag caggcggcgt ctggtggttt aacgtcgatc
    28861 gccatgaaga tgctatcagt ctggcgaatc aaacaattgc atcccaggct gaaaccgcac
    28921 acgtcgcggt cattagcatg gacagcgatc cggcgaaaat ctttcaatta gatgattctc
    28981 aagggccgga aaaaataaaa ttattttcaa tgctaaatca tgaaaaaggt ctatactatt
    29041 tgacccgtga tttgcagtgt tctattgatc cccataatta cctttttatt cttgtttgcg
    29101 caaataacgc atggcaaaac attcctgccg agcggcttcg ctcatggttg gataaaatga
    29161 ataaatggag caggttaaac cattgttcgc ttttggtaat taatcccgga aataataacg
    29221 ataaacaatt ttcattgttg cttgaggaat accgttcact ttttggtctt gccagtttgc
    29281 gttttcaggg tgaccaacat ttgctggata ttgccttctg gtgcaacgaa aaaggggtca
    29341 gcgcccgtca gcagcttagc gttcagcaac aaaatggtat ctggacatta gttcaaagcg
    29401 aagaggcgga gatccaacca cgcagcgacg aaaaacgcat tctgagtaat gttgctgtac
    29461 tggaaggtgc gccgccgcta tcggaacact ggcaactgtt caacaataac gaagtcctgt
    29521 tcaatgaagc ccgtaccgct caggcggcga cggtggtctt ttctttacag caaaatgcgc
    29581 aaatcgagcc actggcccgc agcattcata ccctgcgtcg ccagcgcggt agtgcgatga
    29641 aaatcctcgt gcgggaaaat accgctagcc tgcgcgccac cgatgaacgt ttgttattgg
    29701 cctgcggtgc aaatatggtt attccgtgga atgcgccact ctcccgttgt ctgacgatga
    29761 tcgaaagcgt gcaagggcag aagtttagtc gctatgtgcc ggaagatatc actaccttgc
    29821 tgtcaatgac ccagccgctc aaactgcgtg gtttccagaa gtgggatgtg ttctgtaatg
    29881 ccgtcaacaa catgatgaat aaccctctat tacctgccca cggtaaaggc gttctggttg
    29941 ccctacgtcc ggtaccgggt atccgcgttg aacaagccct gacgctgtgt cgccctaacc
    30001 gtaccggcga tatcatgacc attggcggta atcggctggt gctgtttctc tcattctgtc
    30061 ggattaacga tctggatacc gcgttgaatc atattttccc attgcctact ggcgacattt
    30121 tctcaaaccg tatggtctgg tttgaagatg atcaaatcag tgccgagctg gtgcagatgc
    30181 gcttgcttgc cccagaacaa tggggcatgc cgctgccttt aacgcaaagt tctaaaccgg
    30241 tcatcaatgc cgagcacgat ggtcgccact ggcgacgaat accagaaccc atgcgactgt
    30301 tagatgatgc tgtggagcgc tcatcatgat gaccatcagc gatatcattg aaattattgt
    30361 cgtttgcgca ctgatatttt tcccgctggg ctatctggcg cggcactctt tgcgacgcat
    30421 tcgcgacacc ttacgtttgt tctttgctaa acctcgttat gttaaaccgg ccgggacgtt
    30481 acgccgcacg gaaaaagcca gggcaaccaa aaaatgactc aatttacgca aaataccgcc
    30541 atgccttctt ccctctggca atactggcgc ggcctttccg gctggaactt ctattttctg
    30601 gttaagttcg gcctgttgtg ggcgggatat cttaacttcc atccgctcct caatttggtg
    30661 tttgccgcgt ttctgctgat gcccattccg cgctacagcc tgcatcgctt gcgccactgg
    30721 attgccctgc cgatcggctt tgctttgttc tggcatgaca cctggttgcc tggcccggaa
    30781 agcataatga gccagggttc gcaggtggcg gggttcagta ccgattattt aatcgacctt
    30841 gtcacacgct ttattaactg gcagatgatt ggggccattt ttgttttatt agtggcctgg
    30901 ttattcctgt cacaatggat tcgcattacc gtttttgtgg ttgccatact gctatggctg
    30961 aacgtactta ccctggcggg accaagtttc tccttgtggc cagccggaca accgacgacc
    31021 actgtaacaa cgacgggtgg taacgcagcg gcaaccgttg cggcgacggg tggcgcaccg
    31081 gtagtgggtg atatgcccgc acaaactgca ccgccaacaa cggcgaacct taacgcctgg
    31141 ctgaataatt tctataacgc ggaggcgaaa cgtaaatcga ccttcccgtc ttcgctgccc
    31201 gctgatgctc agccatttga actactggtg attaacatct gttcgctttc ctggtcggat
    31261 atagaagccg ccgggttgat gtcgcatcca ctgtggtcgc atttcgatat tgagttcaag
    31321 aactttaact ccgccacctc ctacagtggc ccggcggcga tccgtttact gcgcgccagc
    31381 tgcgggcaga cttcgcacac taatctgtat caaccggcaa ataacgactg ctatctgttt
    31441 gataaccttt cgaaactggg ctttacccag cacctgatga tggggcataa cggccagttc
    31501 ggcggttttt tgaaagaagt tcgcgaaaat ggcggcatgc agactgaatt gatggatcaa
    31561 acaaatctgc cggttatttt gctgggcttt gatggttcgc cggtttatga cgataccgcc
    31621 gtgcttaacc gctggctgga cgttaccgaa aaagataaaa acagccgtag tgccacgttc
    31681 tacaacacgc ttccactgca tgacggcaac cattatccgg gggtcagcaa aacagcggat
    31741 tacaaagcgc gggcgcagaa attctttgat gaactggacg ccttctttac tgaactggag
    31801 aaatcgggtc gtaaagtgat ggtggtcgtg gtgccggaac acggcggcgc gcttaagggc
    31861 gacagaatgc aggtatctgg cctacgtgat atccctagcc cgtctatcac agacgtcccc
    31921 gttggggtga aattcttcgg catgaaggca ccacatcagg gggcaccgat tgtcattgac
    31981 caaccgagca gcttcctggc tatctccgat ctggtggttc gcgttcttga tggcaagatt
    32041 ttcaccgaag acaatgttgg ctggaaaaaa ctcaccagtg ggttgccaca aacagcaccg
    32101 gtctccgaga actcaaatgc agtagttatt caataccagg ataaaccgta cgttcgcctg
    32161 aacggcggcg actgggtgcc ttacccgcag taagcacgaa aaaggccgca gagtttaacc
    32221 tctgcggcct gatccgggcg caaattgcca ttacggcagc ctgacgcccg catgacacgt
    32281 tacttccgct tattcagcca gttcacgatc atactggcaa gaatgccagc aatgaccgga
    32341 gccgctaaat catgccagaa ggccatgccc agctctgcga gcgtcatata gccgcctgtg
    32401 ttgtaatgac aacgtttcgc ggctattctt gagttgtttg ggttcaagat tagcccccgt
    32461 tatgttgtca ggtgcatacc tgcaacgtgc gggggttttc tctctccagc aaccaatgcc
    32521 accagggata aagcccccgc aacattgcgc ctcaccggaa cctttccggc ttgccgctga
    32581 ttctacgact gtttttctgt tccgaaatcc atcacttact cactctgtgc tgcgcattcc
    32641 gcagaatatt atccgcctgc aaccgttgca tttttcttac gaaaaaggcc gcagagtttc
    32701 acccctgcgg cctggtccgg gcgcaaattg ccattacggc agcctgacgc ccgcatgaca
    32761 cgttacttcc gcttgttcag ccagttcacg atcatactgg caagaatgcc agcaatgacc
    32821 ggagccgcta aatcatgcca gaaggccatg cccagttctg cgagcgtcat atagccgcct
    32881 gtgttgtaat gacaacgttt cgcggctatt cttgagttgt tcaggttcaa gaatggcccc
    32941 cgtgatgttg tcaggtgcat acctgcaacg tgcgggggtt ttctctctcc agcaaccaat
    33001 gccaccaggg ataaagcccc cgcaacattg cgcctcaccg gaacccttcc ggcttgccgc
    33061 tgattctacg actgtttttc tgttccggaa tccgtcacgt cttcactctg tgctgcgcat
    33121 tccgcagaat attatccgct tgcaaccgtt gcatttttct tacgaaaaag gccgcagagt
    33181 ttcacccctg cggcctgatc cgggcgcaaa ttgccattac ggcagcctga cgcccgcatg
    33241 gcacgttact tccgcttgtt cagccagttc acgatcatac tggcaagaat accagcaatg
    33301 accggagccg ctaaatcatg ccagaaggcc atgcccagtt ctgcgagcgt catatagccg
    33361 cctgtattgt aatgacaacg tttcgcggct attcttgtat tgtttggatg caagaatggc
    33421 ccccgtggtg ttgtcaggtg catacctgca acgtgcgggg gttttctctc tccagcaacc
    33481 aatgccacca gggattaagc ccccgcaaca ttgcgcctca ccggaatcct tccggcttga
    33541 cgctgattct acgactgttt ttctgttccg gaatccgtct catcttcact ctgtgctgcg
    33601 cattccgcag aatattatcc gcttgcaacc gttgcatttt tcttacgaaa aaggccgcag
    33661 agtttcactc ctgcggcctg gtccgggcgc aaattgccat tacggcagcc tgacgcccac
    33721 atgacacgct acttccgctt gttcagccag ttcacgatca tactggcaag aatgccagca
    33781 atgaccggag ccgctaaatc atgccagaag gccatgccca gctctgcgag cgtcatatag
    33841 ccgcctgtgt tgtaatgaca acgtttcgcg gctattcttg agtggtctag gttcaagatt
    33901 agcccccgtt atgttgtcag gtacatacct gcaacgtgcg ggggttttct ctctccagca
    33961 accaatgcca ccagggataa agcccccgca acattgcgcc tcaccggaac cctttcggct
    34021 tgccgctgat tttacgattg ctttttactg tcagcacccg gcaatcttcc tcattgcgcg
    34081 atgccttcca gcttaatttc tttatttgca atagttgcag ttcatatccg ggctgactcc
    34141 agactggtag tgaaatgcgc aattttgtag aattgcgcct cctcgcgacc tccccagtga
    34201 atctgttgcc tcatttttta catactgcgt attcgacttc tccacctgtt gcgcaagaga
    34261 aactgggttt attcattttt gcgaggccga cttctttctg gacaggactt tatgcagcac
    34321 aacacactat cgaaacacaa tcagaaattg ccgtttacac gctacgactt cggctgggtt
    34381 ttattatgca taggcatggc gattggtgcc ggaaccgtgc tgatgccagt acaaattggc
    34441 ttgaagggaa tttgggtatt tattaccgca gcgatcattg cttatcctgc cacctgggta
    34501 gtgcaggaca tttatttaaa aaccctttct gaaagcgatt cctgtaatga ctacaccgat
    34561 attatcagtc attacctggg gaagaactgg ggaattttcc tcggggttat ctactttttg
    34621 atgattatcc acgggatttt tatctactct ctctccgtgg ttttcgacag cgcctcgtac
    34681 ctgaaaacct tcggtttaac cgatgccgat ctttcacaat ctctacttta taaagtcgct
    34741 attttcgccg tactggtggc gattgcgtct ggtggtgaac gattactgtt taagatttcc
    34801 gggccaatgg tggtggtcaa agtagggatt attgtcgtgt tcggttttgc gatgatcccg
    34861 cactggaatt tcgccaatat aaccgccttc ccgcaagcct ccgtcttttt ccgcgatgtc
    34921 ttgcttacca ttccattttg cttcttttct gcagtattta ttcaggtact taacccaatg
    34981 aatattgcct atcgtaaacg ggaagcggat aaagtactgg caacccggct cgcgctgcgt
    35041 acccaccgaa ttagttatat cacgctcatc gcggtgatcc tgttttttgc cttttcgttt
    35101 accttctcaa ttagccacga agaagccgtt tctgcctttg aacaaaatat ctcggcactg
    35161 gcgctggccg cgcaggtgat ccctggacat atcattcata tcacctctac ggtgctgaat
    35221 atctttgccg tactgaccgc attctttggc atttatctcg gtttccacga ggctattaaa
    35281 ggcattattc tcaatctgtt aagccgaatt attgatacca agaaaattaa ctcacgcatg
    35341 ctgactctgg cgatctgcgc ttttatcgtc attacattga cgatttgggt ttcgtttcgt
    35401 gtatcggtgc tggtgttctt tcagttggga agcccgttat atggcattgt gtcgtgcctc
    35461 attccgtttt tcctgatcta taaagtcgca caactggaaa aacttcgcgg atttaaagcc
    35521 tggctaattc tgctttacgg cattttgcta tgcttgtcgc cactgttgaa gctgattgag
    35581 taaaccggag cgcatggccc cggttttgtg agttaacgct gcggattttc atcctgatca
    35641 acagcaaaac aagctaccag ttgaccgccg tagtctttta gctgcggctg caactgggtg
    35701 caggggccga agcgccgacg acagcgggcg ttgaaggcgc aacccggcgg tggattcagc
    35761 gggcttggca gttcaccggt gagtttgatg cgttcgcggc gatcgtccgg gttcaggcgc
    35821 ggcgtcgcgg agagtagcgc ctgagtgtac ggatggcgcg ggttattgaa gatttggtct
    35881 ttcgttccct tctccacgca gcggcccagg tacatcacca tcacttcatc ggcaatgtgc
    35941 tccaccaccg acaggtcgtg ggagataaag acataagaaa gccccagttc ctgctgtaaa
    36001 tccatcatca gattcagcac ttgcgcacgc actgagacgt ccagcgcaga aaccggctca
    36061 tcggcaatca ccacatccgg gtcgagcatc agaccacggg caatagcgat acgctgacgt
    36121 tgtccgccgg agaacatatg cggatagcgg tcatagtgct cggttttcag gccgactttc
    36181 gccatcatcg acagggcttt ttcccgacgc tgttctttgc ttaagctggt gttaataagc
    36241 agcggctctt cgagaatttg cccaactttt ttacgcggat tcagcgaacc gtaagggttc
    36301 tggaagacga tctggatttt ctgccgacgc agcttctgcg cctgcggatc gtgcttaagc
    36361 agatcctgcc cctggtaata cagctcgcca ccggtgggca tttcaatcat cgtcagcaac
    36421 cgaccgaggg tcgatttacc gcagccagat tcgcccacta ctgccagcgt tttgccacgt
    36481 tcaaggttaa acgaaatgcc atccagcgct ttaaccagac gttccggcgc gaacatgcct
    36541 ttcttcaccg gataatgttt tttcaggtcg atagcctgca acagcggttg ttgcgaggtg
    36601 gcctcttgcg tactcatagt gtcggcctcc cggcatcatc aagtgggtaa tggcatttgg
    36661 actgacgccc atcagcgagc atattcagcg ccggttcttc agcgcgacat ctgtcagtgg
    36721 cataggggca gcgcgggtta agcaggcagc cgttcgggcg gtcgtactta ccgggaacga
    36781 cacctggcaa cgacgccaga cgttctttgt cctgagcaaa ttctggcagc gcacgcagca
    36841 atgcctgagt atacgggtga cgcggcgcat ggaagatggc gtgcgcatca ccggtttcca
    36901 ccacctggcc tgcatacatc acgatgattt tatgtgccgc ttccgccacc agcgccaggt
    36961 catgggtaat taacaccagc gccatgttct ctttctgctg tagctccagc agtagttcga
    37021 tgatttgcgc ctgaatggtc acgtccagcg cggtggtcgg ttcatcggca atcagcagtt
    37081 ttggccgaca ggcaatcgcc atggcgatca tcacgcgctg gctcatgccg ccggaaagct
    37141 gatgcgggta aacatccaga cgcgatgccg gatcgggaat accgacctga ttcagcaggt
    37201 caatcgctcg ctgacggcgg gtacttttgt tgccgccctg atgcacctta atcgcttcca
    37261 taatctggaa acccacggtg tagcacgggt taaggctggt catcgggtcc tggaagatca
    37321 tcgccacttc ggcacccacc aggttgcggc gctctttttc tgagatacgc tgcaaatcct
    37381 ggccgttaaa ctccagtttt tctgccatta cgcggcccgg ataatcaatc agccccataa
    37441 tcgccagtga actgaccgac ttaccggagc cggactcacc cacaatcccg accacttcgc
    37501 cctgttttac gctgtagctg atgcggtcta cggcgcggaa cggcgcgctt tcgtcgccga
    37561 aatgcaccga taatttatct acatttaata acgccatctc gaacctctta ctgcttcagt
    37621 ttgggatcga gcgcgtcacg cagaccgtca cccatcaggt taaatgccag caccgtcagc
    37681 aggatcgcca gacccgggaa ggtcacgacc caccaggcgc tttgcgcgaa ctgcaacacg
    37741 tcggagagca tggtgcccca ctcaggcgtt ggcggctgtg cccccatgcc gaggaaacca
    37801 agagcagcca tatcgagaat ggcgttagag aaaccgagcg acgcctgaac aatcagcggc
    37861 gcaaggcagt tcgggaagat gttaataaac atctgacgca tcgccccggc acccgccacg
    37921 cgagacgcgg tgacgtaatc gcggttaact tccaccagca cggcggcgcg ggttaagcgc
    37981 acatagtgcg gcaaggcaac gaaggtcagt gccagcgcgg cgttaccaat cgacgggccg
    38041 aaaattgcca ccagcaccag cgccagcagc agacttggca gcgccagcat gatatcgacc
    38101 acgcgcataa tgatgttatc gaccaggccg ccaaagtaac cggcgatcag gccgagaata
    38161 acgcccataa tcagcgataa cacaactacc agacagccaa ccagcagcga caggcgcgca
    38221 ccgtacatca ggcgcgacag cacatcacgg cctacgtcat cggtgcccag caagtgcgcc
    38281 atgctgccgc cttcctgcca ggctggcggg gcgagcagtg catcgcggaa ctgttccgcc
    38341 gggttatagg gtgcaatcca gttggcaaag atcgcgatga acagcacgat gacgacgtaa
    38401 accagcccga cgaccgcgcc tttgttgcgt ttaaaatagt gccagaactc ctgtaacggg
    38461 gtcatcggca ccggtgcgct aatcacttta ttttcagtaa cctgtgacat gatgtcccct
    38521 tacttcttat gacgaatacg cgggttcacc acgccgtaca gcagatcgac cagcaagttg
    38581 acgaggataa tcatcgtcgc caccagcaat acgccgccct gcactaccgg atagtcgcgg
    38641 cgttgcagtg cgtcaatcaa ccagcgtccc agaccgggcc acgagaagat ggtttcggtc
    38701 agaatcgccc ccgccagcaa tgttcccacc tgcaggccga taacggtcac caccggcagc
    38761 atcgcgttac gcagcgcatg gacgataatc acccgcatgc gggttagccc tttggcgcgc
    38821 gcggtgcgga tgtaatcctc gcccagcact tccagcatcg aggagcgtgt catacgcaca
    38881 atgaccgcca gcggaatagt acccagcaca atggcaggca agatcatatg ggcgacggca
    38941 tcgataaagt tgccgtcttc accccagatg gcggtgtcga ttagcataaa accggttaac
    39001 ggattggagt catcgaggaa caccatatcg ctcacgcgac cggagacggg cgtcaggttc
    39061 cagtgcaccg aaaccagcat gatcagcatc atgccccacc agaagatcgg catcgagtag
    39121 ccggtcagcg ccaggccaac cgctgtgtga tcgaaaatgg aaccgcgttt aaccgcagcc
    39181 agcacaccaa ccggaatacc gactgccgta gcaaaaatca tcgcgcagac gccaagttcc
    39241 agcgtggcct gaaagcgcgg cacgaactct tcccatactg gaattcggct tttcattgaa
    39301 atgcctagat cgccgtgcat tacgccccaa atgtaatgga gatactgctg ccacatcggt
    39361 ttatctaagc cgagttcagc cagcagctgc gcgtgacgct caggggagat cccacgttcg
    39421 cctgccatga tcatcaccgg atcgcccggg atcatgtgga caaaggcaaa tgtgagaagg
    39481 gtaataccga taaacgtggg gatgacgagt cccaaacgtc ggagaataaa ctgcaacata
    39541 acccggattc tctatagtga cccgcagcct ggaactgcgt gtctgtattg ctcacaaatc
    39601 aattcccgcc cttgtcggac agagaatgaa gtattgctgc cggatggcgc tgcacgcaga
    39661 ggcacagcgc caccgggtat ggcttttaat tattcgatag agacgttttc gaagtgatgt
    39721 ttgcctaatg gatcaaccac atagccttta acttctttgc gtaccggttc aaacacggtg
    39781 gagtgagcga tgatcagtgc cggagcctga tcgtgcatca ccacctgcgc ttgcttgtac
    39841 agttcaacgc gtttgttatg gtcgtcggta gcacgcgccg gttgaatcag atcttcaaac
    39901 ggtttgtagc accattttga gtagttagaa ccttgttcag aggcggcgca gctgaacagg
    39961 gtggcgaaga agttatccgg gtccccgtta tcgccagtcc agcccatcat taccgtctga
    40021 tgttcgccat ctttcgcacg cttgaggtac tcaccccatt cgtaggtgac gattttggcc
    40081 tgcacgccca ctttcgccca gtctgcctga atcatctccg ccatgcggcg agcgttcggg
    40141 ttatacggac gttgtaccgg catcgcccac aggtcgatgg agaaaccttt ttccagaccc
    40201 gcttctttca gcaaggcttt cgctttttca ggatcgtagg tgtaatcctg aacgtcgtcg
    40261 ttatagcccc acatggttgg cgggatcagg tttttcgctg atacacccgc gccctgataa
    40321 accgctttga tgatcgcgtc tttgttcacc gcgtaggtca gtgcctggcg aactttcacg
    40381 tcatccagcg gttttttctg cacgttatac gagaggtagc cgacgttcag ccccggcatt
    40441 tccatcaggt tgatggattt atcctgcttc atgcgagcga tatctgccgg gttcgggtac
    40501 ggcatcacct ggcattcatt cttctgcaat ttcgcgtaac gcacggaagc atcaggggta
    40561 atagagaaga ccagcgtatc gatctgcggt ttggtgcccc agtagccatc aaacgctttg
    40621 tagcggatac gggaatcttt ttggtactgc tgtaactgga atggaccggt tccgattggg
    40681 ttgaggtcca gtttttccgg tgtaccggct ttcatcatcg catcagcata ttcttttgac
    40741 agaatagagg cgaagtccat tgccagatca gcgaggaacg gcgcttccgg gcgagtcagc
    40801 acgaactgaa ctgtgttgtc gtccaccttt ttcacttcgc tgatcagctc cggcaagccc
    40861 atgccttcga agtattcgta gctaccgcca gaaactttat ggtacgggtt ttgcgcgttt
    40921 ttctgacgat cgaacgagaa caccacgtca tcggcgttca gttcgcgcgt cggtttgaat
    40981 tctttattgt catgccactt cacaccttta cgcagatgga aggtataggt tttaccgtct
    41041 tcgctgactt cccacttttc agcgaggccc gggatcactt cagtggtgcc gattttaaat
    41101 tcaaccaggc ggttataaag cggtacggaa gaggcgtcat aggtggtgcc ggaggtaaac
    41161 agctgcgggt taaacccttc cggagatcct tctgagcaat aaaccagagt tttagcctga
    41221 acacttgctg cgacggtcat agccaccagg ctgagaccaa gcttcagcat ccctgacttt
    41281 ttcaaggaaa tacgcattat tctgctccaa ttgtgatgtt tgttgtttta accctttgca
    41341 gtgggttgtc gctgcctgac cttttttgtt ttttgcccgg tcgggtcaac gttatgaggt
    41401 ggggatgccg tactaattaa catcagtgta acagtgcgga tcctccataa aatgcccctc
    41461 gtgacctaca atctgtcaac agaatgtgaa aacgtcaata cagccaaccg ggatttacac
    41521 caacggtgag aatccacaaa caaagattaa aaaaacttca aacagctatt tgcagcagag
    41581 aaatttgtgc tactccaaca tgaccagaac aatcagctta atatttagca acatttggtg
    41641 attaagtttt atgcatttaa tgaaaaaaat ctgaggaaaa ggtggatatc tgagcgatta
    41701 ataccatgaa cgctaaaacg cacagcggaa aatgccaggg tcagccataa gtaacgcaaa
    41761 aaaagattta agcaaatata aaaaaagaca atggtttctg tcacaaatcc gttaatggat
    41821 agtgagatat ggggcgcaaa tttggcaggg aaatgtgtgg tgtgtaggtc gaataaggcg
    41881 gtcgcgccgc atccgacaat aagtgcggtg ccgttgcctg atgcgacgct ggcgcgtctt
    41941 atcagtatct acgttaattc tgcaatatat tgaatttgca tgactttgta ggcctgataa
    42001 ggcgctcacg ccgcatccgg catgaacaac gcgcacgttg tcagcaatct gattgacatc
    42061 attttttccc attaccattc ctcgcgcagc ttacgctgat attcgacacc atcttcctca
    42121 cacccttgcc agatgccagg aacactggtt cttgctgttt gcgattgatt tatcaggtat
    42181 tgatctaccg cttcccttaa taggtccgca cgaggaagat tacgctgcac ctcgagatca
    42241 tcaagttgct taatcacctc attcgataaa tcgagtaaaa ttctgcccat atctatacct
    42301 gccagagagt tcataaatat ctcgccaata tcatttttga atctatggag agaaaagtac
    42361 ccttgccgaa tctttaaaga aagctcattt acgcatcatt ttttattttt gcgaggcgtt
    42421 ttccagatac gatgtaacag ttgcagttat tataagttca agtaaaacag ccttgtatta
    42481 ataccagaat aagggcatct gtttttttat attcaagaat gaaaaatttt tttcattcct
    42541 tatgctcctt acactgatca tcaacaccaa ctacctcccc cttttgcctg cctccctttg
    42601 ctttacgcac cggaaattgc tgccgctcca gatattgctc aaccgcttcg cgtaaaattg
    42661 cagatcgtga aaccttacgc cagccccgaa gtttgttcag ccgtttaatt gcctcttctg
    42721 ataaatgaag aaaaaccgta ttcatcgcca ttaccaccac cttacctata ttactcatta
    42781 atgtatgtgc gaaaattacg ccggaaaatc atttaaaaaa agcggatttt tgcatgttgt
    42841 tttgtttttg cgaggcgttt tccagaaata ataagagtgt tgcagttgtt tcatttatta
    42901 ctcaaaaatg gtttagttat cgcccaggat gaattatggg tgctatgaat atattatatt
    42961 cagagatatt tatttgcata atctgaatgg tgtagccgaa aaacgtcagc atcctaacag
    43021 caccaggatt taaggtgaaa ttaatcatcc atcacagacc ggtcagtact gccaccaacc
    43081 ggtctttact tgttcatcgt tataattttg ttggtaacag ttcgggaaac aaggaattcc
    43141 ttccggaacc ccccggtatc atccgatagc attgacgtca atcatcagcg cgacgatgag
    43201 tttttttaat cgcatcatag ggtggcgacg ttcagcacga ttacgggtca tagcaaaact
    43261 cctgggcagg cgaccactat gccgctttca caggagttct gaatgctagt gctttctgta
    43321 tggcataacc atgcctctaa cggttaagtg cttgcagcac atgttgcgga tcattggcaa
    43381 tcgctcgcag caaagccttt gcaggcccgg tcggtgaacg tcgcccctgc tcccagttac
    43441 gcaacgtgtc tacgcttaca gaaataagat cggcaaattt agattgtgac aaccctgaag
    43501 cgcggcgtat ttctttgatt ttcatagcat caacttcaaa agtccgcgaa ggctgtcgcg
    43561 ttccttcaat aatttcattc atctgggcca tgctgtgagt caaccgctca aaaagtgcct
    43621 tatccattta ccacctcgta tttaacaacc ggagcattgc tttttcctgg ggagacagat
    43681 catctttaat acccttacga taaataagca gtaatctgat ctcataatgt ttaacctgat
    43741 gaaagtaaat aaccctgaca ccagcacgtt tcccctttcc tcctgaaacc catcgaacct
    43801 ttcttaaccc accagtttca ggtattacct caccatagtc aggattcaag gccaggaaaa
    43861 actgaaaacg actgaattca tcgtcggtca gcagtttttg aacatcttca gtaaaaattt
    43921 ccgtttcgat gaagagcata attatacgcc attggcttag agtaacaatc ctttttgtac
    43981 gtcattgacg tacccttgaa accactagct tactgatagt gctccagcaa aaccacgtct
    44041 ctgccgggtt tgtctttaga tttgcgaggc gctttccaga tttttcacgc ccgtgaattt
    44101 ccctgtatcc atccagcaaa aacgggtgta taagactgta cattcataca gcaaaataag
    44161 ttctactatg gcactggaaa aaggaattgc agagttagtc gaaaagttta tcgctgcggg
    44221 tcgcccctca tcgcgtcaac aaagtattgc gcagcgcagg gagggctata tcgccagcgc
    44281 tgtccttgcg ggtgagacag aaacgcgggt agatattcag actatcgaac ttgaagggat
    44341 gaccttacga atagtgtcgc cgctgaatgc cccgacatta ctcccgacga taatttatta
    44401 ctacggtggc tgttttgtta gcggcgggtt cgcaactcac gacaaccagc tgcgccagtt
    44461 agcctattac gggcaatgtc gggttattgc cgtccagtat cggctggcac cggaacatac
    44521 tttccccgcc gcccatgatg atgcgcaacg aggtgcagaa atagtctggc aacacgccga
    44581 acgactcggc gttgataaac aacgtataac tcttgccggt gacagtgcgg gcggacatct
    44641 ggcgctggta actgctttgc gattaaagcg tgctggcgag tggcaaccgg cacaactcat
    44701 cttaatttat cctatgcttg atgcgactgc gcatttcgaa agctacatac gcaatggtca
    44761 cgattacatc atcacacgcg acactttact gagcggcttt gagatgtatc tgccaggcat
    44821 tgaacgcagg catcctgaag ccagccctct ttggcgcgat gatttcaatg gcttgccacc
    44881 tgtacatatc attaccgctg aatacgaccc actttgcgat gaaggtgaag cgttgtatca
    44941 ccgcatgaca gggcaaggag tgcaatgcac ctgccaacgc taccttggcg ttatccacgg
    45001 attttttcag ttaggtggta tcagcgaggc cgcgcgatcg gcactacggg atgttgcctg
    45061 gagagcggga aggtaacttc ggacctgggc cctgaaaagg ttttcagatt gaaaaccttt
    45121 tcagtcaagt aaaacgcccc tactcttctt cgttcccgcc ctcttcatac gggccaaatg
    45181 gcttcgcggg cagaacgata tacgtgcctt caaacactgc acccggcgtc tcgtcgccaa
    45241 agatttcaac ttgcatctgc acccgtgctt ttcgtccgcg cgccagacgg tcgagatcgc
    45301 cgcttaaggc accaaggtcg gctatcgcat gaggtttacc gctaatcggc ttgctgtagc
    45361 ggatatgcgc atccgccaga ataatcgttc cgccgagatg gcgttcgcgc agcatcagcc
    45421 agataagccc ccaaccggtg agcgtcgcca gcgagaataa actcccggca aacagcgtat
    45481 ggtgcggatt ctgattgccg gtttccggca tggtggtgat aaatttttgc ccggtatatt
    45541 gttgaatgcg cacgcccatt ttttcgctta atggaatgtg ttcataccac gcctgttgca
    45601 gctgggcgca ccagtcaccg cgatgcagaa tatcatccag agtggcaacg ggtttaatca
    45661 tcaaaaaatg gcgaatcggc gtggtagttg gggtggtgat ctccccctga ttaatgaatc
    45721 ccagcttggc gaaaaactcc accgcgtctt cacgggcgct acaggtcacg cgcttaacgc
    45781 cttcctgacg cgccaccgac tccagggtca tcgccatcag cgttcctaac cctttgtcct
    45841 gcacgtcggg atgaacggcc ataaagcgaa tggacgcttc attgtcggca ttaatataca
    45901 atcggcctac cgccaccaga ttaccctgct cgtcgacgac catctggtga tgcgccatcg
    45961 catcccacgc gtcgcgttcc gaaccttttg gttgatgcag gggcttacgc aacatttccc
    46021 agcgaaactg atagtaacgc tctaattctt cttctgtttg tggaacccga aggtgataca
    46081 tagcgatact ctctcttgtt tcccgtgaca accctggaag ctggctcata cctgcaacca
    46141 gaatgtcacg gggccatcat tgaccagcga tacctgcata tcggcagcga agcgtcctgt
    46201 ttgcgtgttc atctcttgct ggcggcagcg ttcgacgaaa tagtcatata acgcctctgc
    46261 gcgatccggt gatgcgcctt tggagaaact tgggcgcatc cctcgttcgg tatctgcggc
    46321 gagggtaaac tgggaaacca ccagtacact gccgcccgcc tgttgcacgt tgagattcat
    46381 cttgccttcg gcatcgctaa agatgcggta gccgagcaca cgctcgcaca gacggtttgc
    46441 tttctgttcg tcgtcatcct tttcgacacc caataacacc aaaagtcccg cgccaatttc
    46501 gcccgtcact tctccctcca cggtgacgct ggcacgggtt acgcgttgaa ttaatgcaat
    46561 catggttcgt cgtcttcttc ttgttcagct gcttgtttta gtttgcggta ttccccgaga
    46621 gtgacagtaa tttccgcgcc aagcaagacg atacaccatg tccagtagac ccaaacaaag
    46681 agaatgggga tcaccgccag cacgccgtaa atgagctgat atgacgggaa catggtgata
    46741 taaagcgcga aacctttctt tcctgcttcg aacaggagtg cagcgacaaa cgcgccgaca
    46801 atcgcgtcgc ggttaggtac gcggatggta ggaacaatgc tgtacagcaa ccagaaggag
    46861 atccacgaca acagcagcgg aaaaatacgc agcacgttgt cgatgacagt attgagatcg
    46921 ctcgcccagc gcagggagag caaataggaa ctgatcgcca gactggcccc tgccagcagc
    46981 ggccccagcg ttaaaatcat ccagtacacg gcaaacgagt aaatttttgg tcgcgctcgt
    47041 ttactgcgcc agatggtatt caacgcgcta tcgatggagt acatcaacaa taatgcggtg
    47101 acgatcagcc cgcacgcgcc aacggcggtc atcttgttgg aattggcaac aaattgttcg
    47161 atataccgct gaataacatc gccagtagca ggcagaaagt tggcaaaaat aaagtgacgc
    47221 aactgaatgc tgacgtcgga aaacatggga aaagcggcga aaagcgcaaa aacaacggca
    47281 accagcggca ctaatgagag caaagacaca taggcaaggt tacctgccag ggttgtcatg
    47341 ttgtcctcat caatacgttg ccagagtagt tttagccagg cccatagtgg acgagtacgg
    47401 tgcctggctt tgtcctgaat ggtttttagc ataacacctt cgcgaaatag tccgggatgg
    47461 tggttttatc tttcaccaga atactggtaa tgcccagctg attggctcct tctatattat
    47521 cggcgttatc gtcgaaaaag accgtatcgc tgggtgaaaa accttccgcc tgcaaaacat
    47581 gctggtaaat tcgtgcttca ggtttgcgca tccccagatc ttgcgacaga tagatatggt
    47641 cagcagcatc acgaatttcc gggtattctt ccggccagaa ggtggtatgc aggcggttgg
    47701 tattggaaag caccaccacg cgatgcccct gctcacgcag tttatgcatg atggcgatca
    47761 cttccgggcg cagcgcaaca aacaccgcct gccagccgtg agagaactgc tcgtagctta
    47821 gcggtagagc catctcatga cacagcgcct ctgcgaacgc ttcgtcgcta atttccccac
    47881 gctcatgctg atgaaacgcc tcccccatat gaaaactctt cttaagcgat gccagcggaa
    47941 tacgcgttaa atcgctccag gctcccagca cacggttaaa gtcgatatcg acaatcacat
    48001 tacctaaatc aaagatatag agcatttttg cctcctttcg cgccacaaga aattaactgt
    48061 agcgggaaag gatgggtttg gctatgtggc aaggtatgaa gccaggttaa cggtcacaaa
    48121 aatctacgcg tcttcctggg gaagaatgat ttcaatttgg ctgtcttgta agcgtgattt
    48181 tagcgcagcg ggcggcagct cgtcggtaaa gagcgcagtg acctgtgcca cgttaccaat
    48241 ttcaaccgcc gccgaagcat gatacttagt gtgatcggcg accagcagaa tatttctcgc
    48301 gtgcgccatc atcgttttca ccacgttagc ttcgtttaca tcaaactcca tcaacgcgcc
    48361 atcgctctca atcgccccaa cgcttgttac cagataatca gcacgaaaat cagccacaaa
    48421 ggacgccgct gaagggccaa tgatcccgct attatgagag cgcaacgtac cgccgggcac
    48481 catcacttca aagcgcgggt tgtggtaaag aatatgcgcc acacgcaggc tgttggtgat
    48541 tatccgcaaa tgattatggt taagtaacgc ccgggcaaca tgctcaacag tcgtaccaat
    48601 ggtgataaat attgttgaac catcaggaat atagtctgcc accgcttcgg caatcgcttt
    48661 tttttcctcg gtttgcgaaa cctcacgctg ctcgaacgcc gtattaacga cgctggaagc
    48721 ccgacccgcg ccaccgtgat ggcgcgtaat caggccttgc tcgcttaatt tacgaatatc
    48781 ccggcggacc gtctgcgtgg agacatccag cagatttgcc agctcatcaa tattcatata
    48841 cccacgctcg gcgatcagca tgaggagttg gtcgtgccgc gggttaccgg ttagttcggt
    48901 aaggctcatg aaaattcctc gaaaaaccat caccctggca ttttatacaa aaagtgacaa
    48961 aaaagatcgg gtttgatcac agtcagggat cccggctcgt ccaccgggac gtgtgcattt
    49021 taacgccgct acaccgctgg cgaagcggac tgactccgct aaatccccac ttgttgccag
    49081 cgccaccgcc aaagcgccgt gaaaaacatc acccgcacct gtggtatcta ccacatcaac
    49141 tttgaaggcc ggttgatgct gacgcccacc attttccagc cagtcgcagc ctgcgctacc
    49201 ctgggtcaca tagacatgac catttgtgag cgtttgtgcc tgttttagcg cactggccat
    49261 ctctttcacg cccgttaagc gcgccagacc cggttctgaa aaggccgcgt gatcgcttaa
    49321 tgccaccagc tcactgatat cctgcggcgt aatgtccccg tccagaacgg tcatcacacc
    49381 cgcctgacgg gccagggtga aggctttttt agcgccgtcg tgccagcgta catctgccag
    49441 cacaacatcc cactgagaga aatcaatttc ctccaaccac tctgcgtcag gcagcagatc
    49501 cgggctgggg tagttaatga ttatccgctc gcctttggta tccaccatga tggcggattg
    49561 cgaagatttc gcctggttat accgtttggt gtaacgggtg ttaacgcccc aggattccag
    49621 ctctgccagc aggctgttgc ccgtgtcgtc atcacctacg cgaccaataa aatcgacctg
    49681 cgcccccagc cttgccgccg caaccgctgc cgtcgccgct ggcccgccac caacttccgt
    49741 ataatttctc gccacgtatt taccgctctc cgtcggtaac ccttccacgt aatagatgcg
    49801 atccatcacg gttataccta cacaagcaac acgaatcatg gtcattcctt aaacattttg
    49861 ggatgcgttc attttaattt caccaattgt ttaaaaagtg acggctgtca attttttgac
    49921 gataaattac aaatacgatc aaaaacagac aagcaaagca tgtcataaat gacaaaaagt
    49981 gacatcactg tattcaggag agggttatgg cagcaatcgc gtttatcggt ttaggacaaa
    50041 tgggttcgcc aatggcgagc aatttattgc agcaagggca tcaacttcgc gtctttgatg
    50101 tgaacgccga agctgtgcgg catctggtag acaaaggtgc gactcccgcc gccaacccgg
    50161 cgcaggccgc taaagatgcc gaatttatca ttaccatgtt gccgaatggc gatctggtgc
    50221 gcaacgtgtt gttcggtgaa aacggcgttt gcgaaggctt atctaccgat gcgctggtca
    50281 ttgatatgtc caccatccat ccgctgcaaa ccgataaatt gattgccgat atgcaagcca
    50341 aaggcttcag catgatggat gttccggtag gccgtacttc tgcaaatgcc attaccggta
    50401 ctctgttact gctggctggc ggcaccgctg aacaagttga acgtgccaca ccgatcctga
    50461 tggcgatggg cagtgagttg atcaactctg gcggtccggg catggggatc cgcgttaagc
    50521 tcatcaacaa ctacatgagc atcgcgctca atgcgctttc ggcagaagcc gccgttttgt
    50581 gcgaagccct gaatcttccc ttcgatgttg ccgtcaaagt gatgagcggt accgccgccg
    50641 gtaaaggtca cttcaccact tcctggccaa acaaagtcct cagcggagat ctttctcccg
    50701 ccttcatgat cgatcttgcc cataaggatc ttggcatcgc ccttgatgtc gccaaccagc
    50761 tgcatgtgcc aatgccgctg ggggccgcct cacgggaggt ttatagccag gcgcgcgcag
    50821 cgggtcgcgg tcgccaggac tggtccgcca ttctggaaca ggtccgtgtc agtgccggga
    50881 tgactgccaa agtaaaaatg taacgactgg ataaaggaat aaatgaatga ataagtacac
    50941 catcaacgac attacgcgcg catcgggcgg ttttgccatg ctggcggtcg atcagcgcga
    51001 agccatgcgc atgatgtttg ccgcggctgg ggcacccgct ccggtagccg atagcgtttt
    51061 aactgatttc aaagttaacg ctgcaaaggc cctctcgcct tatgcctcgg cgattctggt
    51121 agatcaacaa ttctgctacc gccaggtggt tgagcaaaac gcgattgcca aaagttgcgc
    51181 catgattgtc gccgccgatg agttcattcc tggcaacggt attccggtcg atagcgtggt
    51241 tattgaccgc aaaatcaatc cgctacagat caaacaagac ggtggcaaag ccttaaaact
    51301 gctggtgctg tggcgtagtg atgaagatgc gcagcaacgt ctggatatgg tgaaagagtt
    51361 caacgaactg tgccactcac acggtctggt aagcatcatt gagccagtcg tccgcccacc
    51421 gcgtcgtggc gataaattcg atcgcgaaca agcgatcatc gatgccgcca aagagctggg
    51481 cgacagtggc gctgacctct acaaagttga aatgccgctt tatggcaaag gtccgcaaca
    51541 agagcttctc tgtgcttcac aacgtctgaa tgaccatatc aatatgccat gggtgatcct
    51601 ctcttccggt gtcgacgaaa aactgttccc gcgtgccgta cgcgtggcaa tgacggcggg
    51661 cgcatcggga ttcctggcag gccgtgcagt ctgggcatcg gtcgtcggtt taccagacaa
    51721 cgagctgatg ctgcgtgacg tttgcgcacc gaaattacaa caacttggcg atatcgtcga
    51781 cgaaatgatg gctaaacgcc gctaagatga ggataaaagg aatgaaatgg tttaacaccc
    51841 taagccacaa ccgttggctg gaacaggaaa ccgaccgcat ctttgatttt ggtaaaaatt
    51901 ccgtagtgcc gactggtttt ggctggttag gcaataaagg gcaaatcaaa gaagagatgg
    51961 gcacccatct gtggatcacc gctcgtatgt tgcacgttta ttccgttgct gcggcgatgg
    52021 gtcgacctgg cgcttactcg ttggttgatc acggtatcaa agccatgaac ggcgcactgc
    52081 gtgataaaaa atatggcggc tggtatgcct gcgtgaatga cgagggcgtg gtggatgcct
    52141 ccaaacaggg ctatcaacac ttctttgctc tgctgggtgc cgccagcgcc gtcacaacgg
    52201 gtcacccgga agcgcgcaag ctgctcgatt acaccattga aattatcgag aaatatttct
    52261 ggagcgaaga agagcagatg tgcctggaat cctgggacga agccttcagc aaaaccgaag
    52321 agtaccgcgg cggcaatgcc aatatgcacg cggtggaagc tttcttgatt gtttatgacg
    52381 tcactcacga caaaaaatgg ctggatcgcg cgattcgcgt ggcttccgtg attatccacg
    52441 acgtcgccag aaataatcat tatcgcgtta acgaacactt cgatacccag tggaatccgc
    52501 tgccggatta caacaaagat aacccggcgc atcgcttccg cgcgtttgga ggtacaccag
    52561 gccactggat cgaatggggc cgtttaatgc tgcacatcca tgctgccctg gaagcccgtt
    52621 gcgaacaacc accagcatgg ctgctagaag atgccaaagg tctgtttaac gccaccgtgc
    52681 gcgatgcctg ggcacccgat ggcgcggacg ggattgttta taccgttgac tgggaaggaa
    52741 aaccggtggt ccgcgaacgt gtacgttggc ctatcgtcga agcaatgggt acggcctacg
    52801 cgctctacac cgtcactggc gatcgccagt atgaaacctg gtatcaaaca tggtgggagt
    52861 actgcattaa gtacctgatg gactatgaaa atggttcctg gtggcaggag ctggatgcgg
    52921 acaataaggt caccaccaaa gtctgggacg gcaaacagga tatttatcac ctgctgcatt
    52981 gcctggtgat cccgcgtatc ccgttagcgc ccggcatggc tccagcggtt gcagcgggtc
    53041 tgctggatat taatgcgaaa taactgatct gaaaataggg tgcgggtttt cccctcaccc
    53101 taaccctctc cccaaagggg cgaggggacc gtccggtgca gtttttgggt ttgacatcag
    53161 cctcacggga ggctgtatgt cgttaattaa ggtgccttgt atgcaaatca ccaatatgca
    53221 ttgcagtgga cagaccgttt cacttgccgc gggggattat cacgcgacca ttgtgaccgt
    53281 cggtgctggt ctggctgaat taacctttca gggttgtcat ttagtcatcc cgcacaaacc
    53341 cgaagagatg ccgctggcgc atctgggtaa agtgcttatt ccgtggccga accggatcgc
    53401 caacggctgt taccgctatc aggggcagga atatcagtta cccattaatg aacatagttc
    53461 aaaagccgct attcacgggc tgcttgcctg gcgagactgg caaattagtg aattaaccgc
    53521 caccagcgtt accctgacag cttttctgcc gcccagttac ggctatccgt ttatgctggc
    53581 ttcacaagtt gtttattcgc tcaacgcaca cacgggtctt tccgttgaga tcgcaagcca
    53641 gaatatcggc actgtcgcag cgccttatgg cgtgggtatt catacttatt taacctgcaa
    53701 cttaacatct gttgatgaat atctgttcca gcttccggca aaccaggttt atgccgttga
    53761 tgaacacgta aatccgacaa cgcttcacca cgttgatgag ctggatttaa atttcacgca
    53821 ggcgaaaaca atagccgcga caaaaatcga ccacaccttt aaaaccgcta atgatctgtg
    53881 ggaaataacc attacccatc cacaacaggc gctttcggtt agcctttgtt gcgatcaacc
    53941 ctgggtacaa atttatagcg gtgaaaaatt acagcgtcag ggactggcag ttgaacctat
    54001 gagttgcccg cctaacgcct ttaattcagg tatcgactta ttattactgg aaccaggaaa
    54061 accgcaccgc ctgtttttta acatttacgg acagagaaaa taacttgtat tgattagcag
    54121 tcaaaatcat tgaagaacat ttcactcgct aaaggcaaca ttatgtatct tcaatttatt
    54181 aagttaaaaa cagctcagct tctgtaaagt ttttatatat cagttttatg agaatcattt
    54241 tacataagcc tgtaataaca ggaaggcaac aatctcgttc tcaactctat ggagattagt
    54301 tatggatacg ccacgtccac agttattaga ttttcaattt catcagaata acgacagttt
    54361 taccctacat tttcaacaac gtcttatttt aacccatagc aaagataatc cttgtttatg
    54421 gattggctca ggtatagcgg atatcgatat gttccgcggt aatttcagca ttaaagataa
    54481 actacaggag aaaattgcgc ttaccgacgc catcgtcagc cagtcaccgg atggttggtt
    54541 aattcatttc agccgtggtt ctgacattag cgccacgctg aatatctctg ccgacgatca
    54601 gggccgttta ttgctggaac tacaaaacga caaccttaac cacaaccgta tctggctgcg
    54661 ccttgccgct caaccagagg accatatcta cggctgcggc gaacagtttt cctacttcga
    54721 tctgcgtggc aaaccgttcc cgctatggac cagtgaacaa ggcgttggtc gcaacaaaca
    54781 aacctatgtc acctggcagg ccgactgcaa agaaaatgcg ggcggcgact attactggac
    54841 tttcttccca cagcctacgt ttgtcagcac gcagaagtat tactgccatg ttgataacag
    54901 ttgctatatg aacttcgact ttagtgcccc ggaataccat gaactggcgc tgtgggaaga
    54961 caaagcaacg ctgcgttttg aatgtgctga cacatacatt tccctgctgg aaaaattaac
    55021 cgccctgctg ggacgccagc cagaactgcc cgactggatt tatgacggag taacgctcgg
    55081 cattcagggc gggacggaag tgtgccagaa gaaactggac accatgcgta acgcgggcgt
    55141 gaaggtcaac ggcatctggg cgcaggactg gtccggtatt cgtatgacct cttttggcaa
    55201 acgcgtgatg tggaactgga agtggaacag cgaaaactac ccgcaactgg attcacgcat
    55261 taagcagtgg aatcaggagg gcgtgcagtt cctggcctat atcaacccgt atgttgccag
    55321 cgataaagat ctctgcgaag aagcggcaca acacggctat ctggcaaaag atgcctctgg
    55381 cggtgactat ctggtggagt ttggcgagtt ttacggcggc gttgtcgatc tcactaatcc
    55441 agaagcctac gcctggttca aggaagtgat caaaaagaac atgattgaac tcggctgcgg
    55501 cggctggatg gctgacttcg gcgagtatct gcccaccgac acttacctgc ataacggcat
    55561 cagcgccgaa attatgcata acgcctggcc cgcgctgtgg gcgaaatgta actacgaagc
    55621 ccttgaagaa acgggcaagc tcggcgagat ccttttcttt atgcgcgccg gttctaccgg
    55681 tagccagaaa tactccacca tgatgtgggc gggcgaccag aacgtcgact ggagtctcga
    55741 cgatggcctg gcgtcggttg tcccggcggc gctgtcgctg gcaatgaccg gacatggcct
    55801 gcaccacagc gacattggcg gttacaccac cctgtttgag atgaagcgca gcaaagagct
    55861 gctgctgcgc tggtgcgatt tcagcgcctt cacgccgatg atgcgcaccc acgaaggtaa
    55921 ccgtcctggc gacaactggc agtttgacgg cgacgcagaa actatcgccc attttgcccg
    55981 tatgacctcc gtcttcacca ccctgaaacc ttacctgaaa gaggctgtcg cgctgaatgc
    56041 gaagtccggc ctgccggtta tgcgcccgct gttcctgcat tacgaagacg atgcgcacac
    56101 ttacaccctg aaatatcagt acctgttagg tcgcgacatt ctgatcgctc cggtgcatga
    56161 agaaggccgt agcgactgga cgctctatct gccggaggat aactgggtcc acgcctggac
    56221 gggtgaagcg ttccggggcg gggaagttac cgttaatgcg cccatcggca agccgccggt
    56281 cttttatcgc gccgatagcg aatgggcggc actgttcgcg tcgttaaaaa gcatctaatc
    56341 acatccgccc gtggggaaac tcatgggcaa agggagaaca tcgatgagtc acatcacaac
    56401 ggaagatcca gcaactttac gcctgccctt taaagagaaa ctctcttacg gtattggcga
    56461 cctggcctct aacatcctgc tggatatcgg tacactttat cttttgaagt tttataccga
    56521 cgttctgggg ctgccaggca cctatggcgg cattatcttt ttgatttcaa aattctttac
    56581 tgcgtttacc gatatgggta ccggcattat gttggattcc cgacgcaaga tcggtccaaa
    56641 aggtaagttc cgtcctttta ttctgtatgc gtcattcccg gtcaccttac tggcgatcgc
    56701 caactttgtc ggcacaccgt ttgatgtcac cggtaaaacg gtgatggcca ctattctgtt
    56761 tatgctttac ggactgtttt tcagcatgat gaactgctcc tacggcgcaa tggttcccgc
    56821 tatcaccaaa aaccccaacg aacgcgcctc actggcggca tggcgtcagg gcggcgctac
    56881 gctgggcctg ctgctgtgca cggtgggatt cgtgccagtg atgaatctta tcgagggtaa
    56941 tcagcaactt ggctatatct tcgccgccac gctgttttca ctgttcggcc tgctgtttat
    57001 gtggatctgc tactcaggcg tgaaagagcg ttatgtcgaa acccaaccaa ccaatccggc
    57061 gcaaaagcct ggcctgttgc aatctttccg cgcaattgcc ggtaaccgcc cactgttcat
    57121 tctgtgtatt gccaacctct gcaccttagg ggcgtttaac gtcaagctcg ccattcaggt
    57181 ctattacacc cagtacgtac ttaacgatcc catcctgttg tcgtatatgg gatttttcag
    57241 catgggctgt attttcatcg gcgtgttcct gatgcctggc gcagtcagac gttttggtaa
    57301 gaagaaggtc tatatcggcg gcctgctgat ttgggtgctg ggcgatctgc tcaactattt
    57361 cttcggcggc ggctcggtca gctttgtggc gttctcctgc ctggcgttct tcggctcagc
    57421 gtttgttaac agcctgaact gggcgctggt ttccgacacc gtcgagtacg gcgagtggcg
    57481 caccggcgtg cgttcggaag gcacggtcta caccggcttc accttctttc gcaaagtgtc
    57541 tcaggcgctg gctggtttct tccccggctg gatgctgacg caaattggct atgtgccgaa
    57601 cgtcgcacag gctgaccaca ccattgaagg gttacgccag ttgatcttca tctacccaag
    57661 cgcactggcg gtagtcacca ttgtggcgat gggttgcttc tacagcctga acgagaagat
    57721 gtatgtccgc attgtggaag agatagaagc ccgtaaacgc acggcgtaat aataataaac
    57781 gacgccctgc ggggcgttat aaggagtgat tatgtctgac cataatccac tgacattaaa
    57841 actgaatctg cgggaaaaaa tcgcctatgg tatgggcgac gtcggttcga atttaatgct
    57901 ctgcatcggt actctgtatc tcctcaaatt ttataccgat gaattaggga tgcctgctta
    57961 ctatggcggg attatctttc tggtggcgaa gttttttacc gcgtttaccg atatgctcac
    58021 cggattttta ctcgactcgc ggaaaaatat tgggcctaaa ggcaaatttc gtccctttat
    58081 tttatatgca gcagttccgg cggcgttaat tgcaacgctc cagtttatcg ccaccacctt
    58141 ttgtttgccg gttaaaacga cgattgccac cgcactgttt atgatgtttg gactttcata
    58201 cagtctgatg aactgctcgt atggtgcgat gatcccggca attaccaaaa acccgaatga
    58261 gcgcgcgcag ctcgcggctt accgccaggg tggtgcaacc atagggctat tgatttgtac
    58321 cgtggcgttt attccgttgc agtcgctttt ttctgactca accgtcggtt atgcctgtgc
    58381 ggcacttatg ttctccattg gcggctttat ttttatgatg ctgtgctaca gaggcgtcaa
    58441 agagcattat gtggacacag cgccaaccgg acataaagcc agtattctca aatctttttg
    58501 cgcgatattt cgtaatccac cattgctggt tttatgcatt gctaacctgt gtactctggc
    58561 ggcatttaat atcaaactgg cgattcaggt ctattacacc cagtatgtgc tgaatgatat
    58621 taatttattg tcgtggatgg gattcttcag tatgggatgc atcctcgtcg gcgtattact
    58681 ggtgccagta actgtaaaat gttttggtaa aaaacaggtt tatttagctg gcatggtgct
    58741 gtgggcggtg ggtgatatac tgaattattt ctggggaagt aactctttca ctttcgtcat
    58801 gttctcttgt gtcgcctttt ttggcacggc ctttgtaaac agcctgaact gggcactggt
    58861 accagatacc gtagattacg gtgaatggaa aaccggtatt cgtgccgaag gttctgttta
    58921 taccggttat accttctttc gtaaaatttc tgccgcactt gctggcttct tgccaggcat
    58981 tatgctgacg caaattggtt atgttcccaa catcgcgcaa agcgatgcga cattacaggg
    59041 tttgcgtcag ctcattttta tctggccttg tgcactggca attattgctg cattgacgat
    59101 gggattcttt tacacactca atgaaaaacg gtttgcattg attattgagg aaatcaacca
    59161 acgcaaaaat aaagagatgg cgacagaaga aaaaacggct tccgtaacgt tataaatgtc
    59221 aatagccgct atttccattc tggtgggtgg cggcctccct acgtttaaaa aatggactta
    59281 ttatgaaaaa gattaatgca ataattttat tatcttctct gacctctgcc tcggtatttg
    59341 ctggcgcata cgtcgaaaac agggaggcat ataatcttgc ctccgatcag ggcgaggtta
    59401 tgctacgtgt gggttataac ttcgatatgg gcgcgggtat tatgttaacc aatacctaca
    59461 cctttcagcg agaagatgaa ctaaaacatg gctataatga aattgaaggt tggtatcctt
    59521 tattcaaacc aaccgataaa ttaaccatcc agcccggtgg cttaattaat gataagagta
    59581 tcggttcggg tggtgcagtg tatctggacg tcaactataa atttgtacca tggtttaatc
    59641 tgacagtacg aaatcgctat aaccataata actatagttc aacagatttg agcggggaac
    59701 tggataataa tgacacctat gaaattggca cctactggaa ttttaaaatc accgataaat
    59761 tttcctatac atttgagcca cattatttca tacgcgtaaa tgactttaat agcagcaatg
    59821 gaaaagatca tcactgggaa atcactaata ccttccgtta tcgcattaat gaacactggc
    59881 tgccttattt cgaattacgt tggttagatc gtaatgtcga accgtaccac cgcgagcaaa
    59941 accagattcg tatcgggacg aagtatttct tctgatgtga aactgccgga tgcggcgtaa
    60001 acgccttatc cggcctacgg ttgatacggt tttcgttggc cggattaagc acatccggcc
    60061 attaatcaag cttcagccgc cagcatctct ttcttacgtt ggctaattcg cttaaccagc
    60121 attacggaaa ccaccgcacc acagaatgcg aagcaggcca taatgccaaa cacgattttg
    60181 tagccaataa tccccggatt taaatccaga atgtagccat acagactgaa gcagaacatc
    60241 gccggagcgt aaccaataaa gctacccaac gccatcgccg cgcctgtttt attttcagca
    60301 atttttgctt cgccgatagg tgcaaaaaat acggctcgct gtgtaaagac tatcgcgcca
    60361 aagcccagcg tacatgccat ccctaaatag accggcatac tttcgtgcgg cagcataatc
    60421 aacagtacga gcgcagcggt actgatgata aaggtgtagc atagatattt actcggcgat
    60481 ttcaggatct tatctgaaat catgccacca atcggtccac caatcatttt cagacagtat
    60541 tggttaatga tgccgtaagc ccccaccagc gcaacgggca atagatagat gtttttcagg
    60601 aatggaatga agaatgtcag gccacagtaa accgcgtaaa cgaagaagac gttaaaagca
    60661 ataagccaga tagttttgtc tttcagcacc gaggtcatac tggtgttttt cgatgctccg
    60721 tcttcttttt tcacctcgac ggacggtgcc tcttctttgt cattcagaac aaagaaaatg
    60781 ataatgccta cggcaatcac aataagggaa tagaaccaga tgcctgcttt aaaacccaat
    60841 aagccactgc caaaccaggt aaatactgcc aacgcagaaa atgccaccac ggtatcgaca
    60901 atgccacgcc ctgtttcgaa gaagccaaac aaccgacctt gttgttcgct attgcccaat
    60961 cgactcaccg acttgagcaa gaccggccag ttcatcatgt cgcaagtaac gccaaatagc
    61021 gcccagacaa agaggatgcc ccaatacccc ggcattgtcg tcaaataaac acccagtaat
    61081 cctgtcgcaa tgagtgagaa tgacatggtg tatctgcgcg gtagtttatc ggcaaaataa
    61141 atagacagaa aaaaacccac tgtcgtgaca aatgagttta ccgacatagc attaccaatt
    61201 tgaccattgg tcaaatggaa atattcctgc atcgggatat aaaacgcatc tttcagcgac
    61261 ggtaatttat aaattgtacc gccacacaat gttaatagac taaataacgc ccatttcttt
    61321 ttcgtgagca taacatcccc taagcgattt ttaaaatcaa tgttttaaag gcaataagat
    61381 accgaccatt catatggaat ggttttatta actgcgtatg agttaaaact tattagtggt
    61441 tcagaagatg gtttgcctct tgtttgattt gttgtagtac gttacgcaca tgatttatct
    61501 gattaattgc atcttctttt tctttacgta accgcgcatc cagtaaatga ttttctggca
    61561 atggtgtttc actcatctgg cgataaggga tccacggatt atcatcttcg tcttcaaagc
    61621 ggaacgccgg cgcatagtaa tcaacctctt cttccaggcc atatgccgtc acctgcggct
    61681 catcatcacc caggcatata aggtgcgtta ataacgcttt gaacggcata tcaccctgac
    61741 ctgaaataca ggctttatga cccaggccac ccggttcttt aacgatcaag gcatctttga
    61801 tatggacctg ggtaatatgc ggcgccatcg tttttaaagc gtcgatggga tgctcatttg
    61861 cattgatcat gttcgcaaaa tcaaacagta aggaaagaga ttccatctca ctttctttga
    61921 ccagcgacac cagctcatgg ctttttaaat cttcatgctg ttcgatcgta aaagtcaggc
    61981 cgctgtcctg atacgtttcc cgtacatagg caatgtcgtt agcgatgatc gataatacgt
    62041 cgcgcaaatt accttcataa cgtggataaa aacgtacgga cgatgcccca gttttcaacg
    62101 caatggcgac ggcttcgtca atagatgcct tatctgaagc gctggtttca atatgaatat
    62161 caaggttcag acggcgggct ttatccccaa aggcagagag ttctttatcg tccatattac
    62221 caagagaaaa acgctcgcca tccagaacat ggattttgac gccacgtaga ttattttcac
    62281 ttgcgatatc aagtaaatca gcaggcaaca ctctttccag ccgcatattt aaatgaaagg
    62341 cataagcatg taaataaaga ggaagagtgt cgacacgttg tagaatcttt cttgcgttat
    62401 ttattgtcac cataaaacac ctacagatta atgatgaggt ctacggcctc aaatcgccaa
    62461 tattcaatat ctatctggac aatattgcct tgctggtcgg cgcgatgctt ttcaattaag
    62521 atcgctggca tacccgcaga accgccaaga tacttgctga tatcgcccgg cattctgacg
    62581 ggtttgaaaa tcaatttttt tactaccgtc tctttttggt agcacttttc ccagactgca
    62641 gaaaatgagt ggttttcaag ttgttcaata aaacccggag caacttcagg attaatatat
    62701 gtttcgtgat agaaaacttt atgtccttcc agcgcccccc agccggtgat gcgatagagt
    62761 tcagttgatg gtgtcactgc aatcagtggc gcgagcgtct cgggaatatc gctggtacgg
    62821 tttttctcgg taaaccccca ggaaggttcc cgcccttgct caattgcggc ccgctgaaag
    62881 ctcgccgaca gctccgggct gtaattaaat cgcggctgag tcacgaacca ccctttacga
    62941 tccttacgga agattttgga ttctgcctcg aggttcagca acgcctggcg cagcgtcata
    63001 cgtttaatgc ccagcagctc tcccagctct ctttctgaag ggagttttcc acccggcgta
    63061 gtgatgccat cttttaacca ccgatccagt ttctcttttg cattttctac ggtggattga
    63121 tttcccgtca ttgtttaatc cttttggttt aaaccagatg aggcatctta cgcttacgcc
    63181 aaaaaagaaa gcaattaacg gcgtatgact ttgcagataa ttgtgacaaa gctctctatt
    63241 gacgtaacca cagacttata agggagatac tcgcattgct gagtgctcac gacaggcaaa
    63301 aaaaaacctg ccatcgctgg caggtttttt atgactaaaa aacgaaatta atcgtctttc
    63361 ggtgcgcggt tggcgcgacg acgatcgttt tccgtcaggt gacgtttacg aatacggata
    63421 gaggtcgggg tcacttctac cagttcgtca tcatcgatga actccagagc ttgttccaga
    63481 gtcatgcgga taggcggaac cagaacaacg gcttcgtcag taccggaagc acgcatgttg
    63541 gtcagtttct taccggtcag gcagtttaca gtcaggtcgt tagagcggct atgaataccg
    63601 ataatctgac cttcgtaaac ttctgcaccg tgaccgagga acagcttacc gcgatcctgc
    63661 agaccgaaca gcgcgaacgc gaccgcttta ccctgaccgt tagagatcag tacgccgttc
    63721 tgacgctgac ccacttcacc cggacgtacg tcgtcgtagt ggctgaaggt ggagtacagc
    63781 agaccagtac cggaagtcat ggtcatgaac tcagaacgga agccaatcag accacggctt
    63841 gggatcacgt agtcgagacg tacgcggcct ttaccgtctg gattcatgtt tttcaggtcg
    63901 cctttacgtt cgcccagcgc ctgcattaca gaaccctgat gctgttcttc aacgtccagc
    63961 gtcacgtttt catacggctc ttgtttacga ccgtcgattt cacggaagat aactttcgga
    64021 cgggataccg ccagttcgaa accttcacga cgcatgtttt cgatcagaac agacaggtgc
    64081 agttcgccac gaccagaaac gcggaacgca tcggcgtctt cggtttcttc tacgcgcagc
    64141 gcaacgttat gtaccagttc tttgttcaga cgatccagga tctgacgaga cgttacgaac
    64201 ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga agaacataga aacggtcggc
    64261 tcatcaacgg agagtgccgg cagcgcttca acgttttgcg tgtcgcaaac ggtgtcagaa
    64321 atgttcagtt cgccaaggcc cgtgatcgcc acgatatcgc cagcttccgc cagatcggtt
    64381 tcgatacgtt ccagaccgag gtggcccagc actttaccga ctttcgcgtt gcgggttttg
    64441 ccttcgctat cgatgatagt gacctgttgg ttcggcttca ctttaccgcg cttgatgcgg
    64501 ccaatgccga taacgccaac atagctgttg taatcgagct gagaaatctg catctggaac
    64561 ggaccgtcaa ggtcaacgtc cggcgcagga acgtgatcaa caatcgcctg atacagcggg
    64621 gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga taccgttcag cgcagaagcg
    64681 taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga ggttaacgaa cagatcgaat
    64741 acctgatcca caacccaatc aggacgcgcg ccagggcggt caactttgtt gataacaaca
    64801 atcggcttca ggccgtaagc aaacgctttt ttggttacga agcgcgtttg cggcatcggg
    64861 ccgtcaaatg cgtcaaccac cagcagcact gagtctacca tggacattac acgttcaact
    64921 tcaccaccga agtcggcgtg ccccggggta tcaacgatgt tgatacggta atcattccat
    64981 ttgatagcgg tgtttttcgc gaggatggta atcccacgct ctttctccaa atcgttggag
    65041 tccatcacgc gctcttgggt ttcggcacga gagtcgaacg taccggattg ttggagcagc
    65101 ttgtctacca gggtggtttt accatggtct acgtgcgcga tgatggcgat attacgcaat
    65161 ttttcgatca caactttgcc tcaggcatta gaaatagcgc gttattgtac acggattaat
    65221 cgcactacaa aacaggatca caaacatcct ccgcaaacaa gtattgcaga gtccctttgt
    65281 gatcgctttc acggagcata aaaagggtta tccaaaggtc attgcaccaa catggtgctt
    65341 aatgtttcca ttgaagcact atattggtgc aacattcaca tcgtggtgca gcccttttgc
    65401 acgatggtgc gcatgataac gccttttagg ggcaatttaa aagttggcac agatttcgct
    65461 ttatcttttt tacggcgaca cggccaaaat aattgcagat ttcgttacca cgacgaccat
    65521 gaccaatcca ggagagttaa agtatgtccg ctgaacacgt actgacgatg ctgaacgagc
    65581 acgaagtgaa gtttgttgat ttgcgcttca ccgatactaa aggtaaagaa cagcacgtca
    65641 ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga aggcaaaatg tttgacggct
    65701 cctcgattgg cggctggaaa ggcattaacg agtccgacat ggtgctgatg ccagacgcat
    65761 ccaccgcagt gattgacccg ttcttcgccg actccaccct gattatccgt tgcgacatcc
    65821 ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg ctccattgcg aagcgcgccg
    65881 aagattacct gcgttccact ggcattgccg acaccgtact gttcgggcca gaacctgaat
    65941 tcttcctgtt cgatgacatc cgtttcggat catctatctc cggttcccac gttgctatcg
    66001 acgatatcga aggcgcatgg aactcctcca cccaatacga aggtggtaac aaaggtcacc
    66061 gtccggcagt gaaaggcggt tacttcccgg ttccaccggt agactcggct caggatattc
    66121 gttctgaaat gtgtctggtg atggaacaga tgggtctggt ggttgaagcc catcaccacg
    66181 aagtagcgac tgctggtcag aacgaagtgg ctacccgctt caataccatg accaaaaaag
    66241 ctgacgaaat tcagatctac aaatatgttg tgcacaacgt agcgcaccgc ttcggtaaaa
    66301 ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg ctccggtatg cactgccaca
    66361 tgtctctgtc taaaaacggc gttaacctgt tcgcaggcga caaatacgca ggtctgtctg
    66421 agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc taaagcgatt aacgccctgg
    66481 caaacccgac caccaactct tataagcgtc tggtcccggg ctatgaagca ccggtaatgc
    66541 tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat tccggtggtt tcttctccga
    66601 aagcacgtcg tatcgaagta cgtttcccgg atccggcagc taacccgtac ctgtgctttg
    66661 ctgccctgct gatggccggt cttgatggta tcaagaacaa gatccatccg ggcgaagcca
    66721 tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa agagatccca caggttgcag
    66781 gctctctgga agaagcactg aacgaactgg atctggaccg cgagttcctg aaagccggtg
    66841 gcgtgttcac tgacgaagca attgatgcgt acatcgctct gcgtcgcgaa gaagatgacc
    66901 gcgtgcgtat gactccgcat ccggtagagt ttgagctgta ctacagcgtc taatagttga
    66961 agttgtacta cccggcgcaa caacgccggg atttagttgc cgtggaaact ttcagcccat
    67021 ctctgcatgg gcttttttct ccgtcaattc tctgatgctt cgcgcttttt atccgtaaaa
    67081 agctataatg cactaaaatg gtgcaacctg ttcaggagac tgctttatgg caacaggcac
    67141 gcagcccgat gctgggcaga tcctcaactc gctgattaac agtattttgt taatcgatga
    67201 caacctggcg atccattacg ccaaccctgc cgcgcaacaa ctgctcgccc aaagctcccg
    67261 caaattgttt ggtacaccgt taccggaact gttgagctac ttctcattaa atatcgagct
    67321 gatgcaagaa agtctggagg cggggcaagg ttttaccgat aacgaagtga cgctggtcat
    67381 cgacgggcgc tcgcatatcc tttctgtgac ggcccagcgt atgccggacg gcatgatcct
    67441 gctggagatg gctccgatgg ataaccagcg ccgcttaagt caggaacagc tacagcacgc
    67501 ccagcaggtt gctgcccgtg atttagtgcg cggcctggca catgagatta aaaatccgct
    67561 tggcggttta cgtggcgcgg cgcagctact cagcaaagcg ttacctgacc catcactact
    67621 cgaatatacc aaagtgatta tcgaacaggc ggaccggctg cgaaatctgg tcgaccgtct
    67681 gttggggccg cagctgcccg gtacgcgcgt taccgaaagt attcacaaag tagctgaacg
    67741 cgtggtaacg ctggtgtcga tggaactgcc ggacaacgtg cggttgattc gtgattacga
    67801 tcccagcctg ccggaactgg cgcacgaccc ggatcaaatt gaacaggtct tgctgaatat
    67861 tgtgcgcaat gcgctacagg cgctggggcc ggagggtggt gaaatcattc tgcgtacccg
    67921 caccgcgttt caactgacct tacacggcga gcgctaccgg ctggcggcgc ggattgatgt
    67981 ggaagataac gggccgggca ttccgcctca tttgcaggat acgctgtttt acccgatggt
    68041 cagcggccgc gaaggtggca ccgggcttgg cttatccatc gcccgtaatt tgattgatca
    68101 gcattcaggc aaaattgaat ttaccagttg gccaggtcat accgagttct cggtttacct
    68161 gcctatcagg aaataaaggt gacgtttatg caacgaggga tagtctgggt agtcgatgac
    68221 gatagttcca tccgttgggt gcttgaacgt gcgctcgctg gagcgggttt aacctgtacg
    68281 acatttgaga acggcgcgga agtactggag gcgctggcga gcaaaacgcc ggatgtgctg
    68341 ctttcagata tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag
    68401 cgccatccaa tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc
    68461 agcgcctatc aacaaggggc gtttgattat ctgcccaaac cgtttgatat cgacgaagcc
    68521 gtcgcgctgg ttgagcgcgc catcagtcat taccaggaac agcagcagcc gcgtaatatt
    68581 cagcttaacg gcccaacgac cgatatcatc ggcgaagcgc cagccatgca ggacgtgttc
    68641 cgtattatcg gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc
    68701 accggtaaag aactggtcgc tcatgccctg catcgccaca gtccgcgcgc caaagcgccg
    68761 tttatcgcgc tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc
    68821 cacgagaaag gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc
    68881 gatggcggta cattattcct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt
    68941 ttgctgcgcg tgctggcaga cggtcagttt taccgcgttg gcggctatgc gccggtgaaa
    69001 gtggatgtgc ggattatcgc tgccactcac cagaatctcg aacagcgagt gcaggaaggt
    69061 aagttccgtg aggatctgtt ccaccgcctg aacgttatcc gcgttcatct gccgccgctg
    69121 cgcgaacgtc gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc
    69181 gaactgggcg tagaagcgaa gttactgcat ccggaaaccg aagctgctct gacgcgtctg
    69241 gcgtggccag gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc
    69301 gccgggcagg aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacggttgcg
    69361 gagagtactt cgcaaatgca accggacagc tgggcgacgc ttcttgcgca gtgggcagac
    69421 agagcgctgc gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctggagcgg
    69481 acgttactga cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta
    69541 ctcggctggg gccgcaacac cctgacgcgt aagttaaaag agctggggat ggagtgattc
    69601 acagcttgtg tgtaaagatt gattattgag cgcaaattgc tggtatttta cgctttactg
    69661 ttccgataag ttcagtatga tcttgcccgg aaaacgggga gagtcattat gctggaatca
    69721 ataattaatc tggtatcgag tggcgcagtt gacagccaca caccgcaaac tgccgttgct
    69781 gccgtgctgt gtgccgcaat gattgggctg tttagttgag tttcagattg ctgatgaagt
    69841 gctctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcaatattt
    69901 tcaacaggtt ttgtaggcct gataagcgta gcgcatcagg caatcttgtt ctcagcacat
    69961 tgatggcgct ttcgttttct actttgtaaa cgaagcgcca ttcactattt aaatcacccg
    70021 tgagaactgc tgcatccgcg ctttctggcg cagataggta tcaaagcaca tgcaaatgtt
    70081 gcggatcagc aagcgacctt tcgccgtcac ctgtattccc ttctcatcca catccaccag
    70141 cccatctttt gctaacgggg cgagcagctt gagatcttcc gcaaagtaat cagcgaagtg
    70201 caaatcccac tgtttctcaa taggggcgta atccagacgg aagttgcaga tgagcgactt
    70261 aatcacatcg cggcgaatac agtcatcacg cgttagcgca ataccacgcc acagcgcatt
    70321 gccttgttca tccacttgct gatagtactg cttcaactct ttctggttct gcgcgtagca
    70381 gtcgccaatc atgctgatgg cggaaacgcc catccccagc agatcggtat cgccctgagt
    70441 ggtgtagccc tggaagttac gatgcagcac gccttcacgc tgggccaccg ccagctcgtc
    70501 atccggacgg gcaaagtgat ccataccgat aaactgatag cccgattgcg tcaggaaggc
    70561 gatggtttcc tgcaggatat cgagtttttg ctgcggactc ggcaggtcag catctttgat
    70621 tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag ttaaagacac tcagacgatc
    70681 ggggttcagc tccgccacac gtttcagggt aaaggcgaaa ctctccggcg tctgtttcgg
    70741 caggccgtaa atcaggtcga tgttggtgga ggtaaaacca atctcacgcg catggttaag
    70801 cagtgcaaag atgaactctt catcctgctc gcggttaacc agacgttgca cttctttgtt
    70861 gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg gcgcgtaaat gatcgagtac
    70921 atccagttcg atttcccgcg gatcgacttc gatcgaaatc tccgcatcgg cattgaactg
    70981 gaagttttcg cgcagcagct tcatcaggcg gctgatttgc gctttattca gatacgtcgg
    71041 cgttccgccg ccccagtgca attggctgac gtgacgcccg gcgaacagcg gtgcacgatg
    71101 gacgatttct tgctccagcg cgtccagata ctgatcggcc ttgtgctgct ggcgagtaac
    71161 aatcttattg caaccgcaga agtaacaaag cttatggcag aacgggatat gtacgtagag
    71221 agataatgga cgctcaggat agcgcgccac ggcttgtaaa aacgcctgtt cgccgaagtc
    71281 ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt ggcccggaat agttatattt
    71341 ctggatcagg gccagatccc agtcgatttg ctgtacagac atgctcactc cttccgatgg
    71401 cgtctctggc ggctacggcg taccggcgga tgcccgtttc gggtcattag ccggttgcgc
    71461 agccactttt gtcgccgcga caaccgctgt agtttaacga ataaccacac cagataacat
    71521 ataaccggaa gggttatcag cagaacggga aggcccacgg cggcaatccg ttagttgccc
    71581 cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt cctcttcttc gtcatcgtca
    71641 taagagaggc cgagtttctg catcaactca tcaatacgat ccagtttagc atccacccag
    71701 gattgctctt cggcactcag ggtttcgcct gcttccagac gttccagcag cgcatccaga
    71761 cgctcatccg tttccagtaa ttccaactcc gcctgcggtg aaagcatagg tttctcactc
    71821 ttcggtttgt gctgtttggt gactttttca gtcacgccca atggaatagg ggttttactg
    71881 ccaatacgtg gatcttttgg tgcgttctgg cctttgctgc ctgacgtggt gtttccgccc
    71941 gctgcgcggc tgcccggcgc atgaccacga cgttttttct gacgcttgcg atcacgagct
    72001 tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg catgaccttt gctgcgtgag
    72061 tttgaagatg atggtttcat aatggttcgt cttttagccg ttttattcag tatagatttg
    72121 cggcggaatc taacagaaag caagcaaaga aaaaaggcga cagattactc tgtcgccttt
    72181 tttcctgact cataaccctt aacgggtctt acaatccttg caggccgcca acacaccctg
    72241 tttttccttt agccggaaca atccgtgttg attccatttc cgtttaatta cgtctccgga
    72301 cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc gctcctgacc ctcatcctga
    72361 gtcattgttc ctgttagcgt cctcgctcta tgggtgctac tttacgcctt tgctttaaac
    72421 aaacaatcaa ccgccatcag tatttgcaca ttttcagagt tttatataaa agcaattcac
    72481 tgatttatat agcttatttg acatgcatcg cagaattttc tctgctattt cgcataaaga
    72541 ctatcggcaa tgtctcacaa agcgtatggc ttttacttac aacgctcacg ccgaaaaatc
    72601 cgccttttat tatggttcag gtataatcgc cacaaagctt tgactgacgg agacgaccgc
    72661 tttgactaat ttgaattatc aacagacgca ttttgtgatg agtgcgcctg atattcgcca
    72721 cctaccttcc gataccggaa ttgaagtggc ttttgcaggc cgttccaacg caggtaaatc
    72781 cagcgcgctg aacacgctga ctaaccagaa aagcctggct cgtacctcaa aaaccccagg
    72841 gcgcacccag cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg ttgacttgcc
    72901 tgggtacggt tatgcggaag tcccggaaga gatgaagcgc aaatggcagc gtgcgctcgg
    72961 cgaatacctc gaaaaacgtc agagcctgca aggtctggtg gtgctaatgg atattcgcca
    73021 tccgctgaaa gatttggatc agcagatgat tgagtgggcg gtagacagca atatcgccgt
    73081 tctggtgctg ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag cgcaattgaa
    73141 tatggtgcgt gaagctgtac tggcgtttaa cggtgatgtg caggttgaaa cgttttcttc
    73201 gttgaagaaa caaggcgtgg acaagctgcg gcagaaactg gatacctggt ttagcgagat
    73261 gcagcctgta gaagaaacgc aggacggcga ataattttct tgccttaatg cttgtgccgg
    73321 atgtggcgta tccggcccgt aaattcaata gattacaatt ctaccgttgg cctgataaac
    73381 atagcgcatc aggcattacg gatcttttct ttcgcccaat aaaaaacgcc ccagtcatta
    73441 ctgactgggg cggctaaaat attcagccaa atccgattac gtgaagtaaa aggtctgaaa
    73501 gatagaacat cttacctctg taccctacgc gactaacttt actctttttt gttcagttca
    73561 gaaagcactt tttgtaattt tttttcagtt gttgcatagg aatttctaat agccatcaca
    73621 aaacgtgaca gcttatgttg cttacttacg aaaaaagggc atgttcaggc gaatcttagt
    73681 gcgcctgatc ccagttttcg ccactcccca cttccaccag caacggcaca tccagacggg
    73741 tacagttttc catcagttga tgaatctgct tcgcgacggc atcaacatca tctttatgaa
    73801 cttcaaatac cagttcatcg tgtacctgca tgatcatacg tacacgcggt tgctcagcct
    73861 gtaaccacgc atcaacggca atcatcgccc gtttgataat gtcggcggcg gttccctgca
    73921 ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg agcaccattg ctggatttga
    73981 tatccggcag atacagacgg cgtccgtcca gcgtttcaac gtagccctgc tctttcgcct
    74041 gagcacgggt gcgttccata tactccagca cgccagggta gcgttcgaag taaaggtcca
    74101 tgtacttctg cgcttcttta cgtggaatgt tcaattgccg cgccagaccg aaagcactca
    74161 tgccataaat cagaccaaag ttgatcgctt tcgcgctacg gcgttgctcg ctggtgacgg
    74221 tttccagtgg caaaccaaac acttctgccg ccgttgcccg gtggatatct tttccttccg
    74281 cgaatgcggt cagcaagcct ttgtcacgcg aaagatgcgc cataatgcgc agttcaatct
    74341 gcgagtagtc cgctgagaca atcacataat cctctggcgc aataaacgcc tggcggatac
    74401 gacgaccttc ttcgttacgc accggaatgt tttgcaggtt aggatcggtt gacgataaac
    74461 gtcccgttgc agttactgcc tggtgataag aggtatgcac acgcccggtt ttcgggttga
    74521 tcatcagcgg cagcttgtcg gtgtaggtcg atttcagctt cgccagacca cgatactcca
    74581 gaatcacttt tggcaacgga tagtccagcg ccagttcttc cagtacctct tccgacgttg
    74641 acggcgcgcc acccggcgtt ttcttcagcg gtttaatacc ctgtttttca aagagaatgg
    74701 tttgcaactg cttggtggaa gaaaggttaa attcttcacc tgcaatttca tgcgctttct
    74761 tttccagctc agccagacgc aaggtgagct cttcagaatg attgtgcagc actttcggat
    74821 cgatcttcac accgttacgt tcaatgcgtg aaagcaccgg caccagcggc atttcgatat
    74881 tctcgaagac gttcaacggc cctttgtgtt tttgcagatc tggccacatt ttcagatgca
    74941 actgcaaggt gacatcagca tcttcggcgg cgtaacgtcc ggcctcttcg agggcaatct
    75001 ggttaaaggt cagttgattt ttgcctttac ccgcaatctc ttcaaaagtg atggttttgt
    75061 gcttcaacca acgttccgcg aggctgtcca tatcgtgacg cccggcaacg ctattgagaa
    75121 tgtaggactc cagcatggta tcaaacgcaa tcccacgcag ctcaatgccg taattcgcca
    75181 gaataccgcg atcgtatttc aggttttgcc cgaccttcag cgccttttca tcttccagca
    75241 gcggttttag caactcgagt gcacgctcgc gagagatttg atcgggcgcg tcaagataat
    75301 catgagcaac cggaatatat gccgctacgc ctggctcgat agcaaaagaa agcccgacca
    75361 ggttagcaga gatgttatca aggctgtcgg tttcggtatc aaatgcaaat accggcgctt
    75421 tttccagctt cgcaatccac gctttcagtg tttcttcatc aaggatggtg acgtagttgt
    75481 cataagaaat caccgttgcc gtcacttctg gcgcttcgtc tgcaacactg gtttcctgtg
    75541 gcttcgcggc tggttttgcc cctttggcct gtaaccattt gcccgcttcg acatcagcag
    75601 tccagcgttt gaactcatac tttttgaaca gccccaacaa ctcttccgct gccggttgct
    75661 gcacttccag ttgttcacag gtcagctcca gttcaacgtc ggttttaatc gtcgccagct
    75721 ggtatgagag ataagcaact tctttgtttt gctcgagctt cgctgccatt gttttcgcgc
    75781 cacggaagct caacccagca attttttctg gctcggcata cagcgtatcc agtccgccaa
    75841 gaccttgcag caatgcctgc gcggtttttt caccgacgcc cggtacgcca ggaatgttat
    75901 cagaggagtc acccatcagc gccaggaaat cgatgatcag ttctggcggc acgccgtact
    75961 tattcaccac ctcttccggt ccgaggatgg tattcgtcat ggtattgata agcgtaatat
    76021 ttggcgtcac cagctgcgcc atatctttat cgccagtgct gatcagcacc ggacgcccgg
    76081 ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc cgcttctacg ccagaaaccg
    76141 ccagcagcgg cagtcccatc gctttaacca tcgcgtgcaa gggttcgatt tgtgcacgca
    76201 gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc aaacagttca tcacgaaagg
    76261 tttttccctt ggcgtcaaag accaccgctg catgcgtcgg tttatattgc atgatcagac
    76321 tgcgcagcat gttgaggaca ccatacatcg caccggtcgg ctcgcctgcg ctgttagtca
    76381 gcgggggaaa cgcgtgatat gcgcgataaa gataagatga accatctaca aggataagtg
    76441 gattttgggg gatctgaacc ataatgtccg tgcctgttta tcagattatg ggtaaaggat
    76501 gccacagaca ggatgaaaac atgagttttt caccgctttt cgtgaaaatc ttttgtagat
    76561 cttctggatc gctcgcaaat cgtcatgtgg ataactttgt gcataaatta tttacatcgc
    76621 ttttaattta ttatcatgct ttttacagca tgatgtttat ctttgttttt catgcagtta
    76681 aaaataaatc catccctaat aattgagata atgggatttt ttctaatgtg gatagatatg
    76741 aattattttt ctccttaagg atcatccgtt atttgggtcg ttttctgcta aaatctgcgc
    76801 cttgcgcttc tttagcgcac tgtctatggc taaccttttg aataaattca ttatgacgag
    76861 aatactcgct gcgataaccc ttttgctgag tatcgtattg accattttgg tcactatttt
    76921 ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta ttgctacctg tgccagtcat
    76981 ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg aaggtctggc
    77041 ggtattactg catctgaacc cacacctgca atgggaagtt cacgggctgg aagggttaag
    77101 taagaagaac tggtatctgc ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact
    77161 gtgcgtgctg tttcgtaagc acattccgat gaataaatat ttcctcaagc aacagctggc
    77221 ctgggtgcca tttcttggcc tggcgtgctg gtcgctggat atgccgttta tgaagcgtta
    77281 ttcccgcgct tatttattac gtcatccgga gcgacgtggt aaagatgttg aaaccactcg
    77341 gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg ttgaaggctc
    77401 ccgtttcacg caagaaaaac atcagcaaac ccactccact tttcaaaact tgttgccacc
    77461 aaaggcggca ggcattgcga tggcgctaaa tgtactgggc aaacaattcg ataaactgct
    77521 taatgttaca ctgtgttatc cggacaataa ccgtcagccg ttcttcgata tgttaagcgg
    77581 taaactgacg cggattgtcg tacatgtgga tttacagcct attgccgatg agttacatgg
    77641 cgactacatc aatgacaaaa gctttaagcg ccattttcag caatggctga actcattgtg
    77701 gcaggaaaaa gaccggcttc tgacgtctct catgtcatca cagcgtcaga ataagtaagt
    77761 tttactcgcc tctgaatgag cagaggcgag tgagtatttt aatgaacaaa acgtccggca
    77821 cgagacataa attcttcttc gctcatctcc tgtccgttaa aaacaacttt acccggtgta
    77881 taacgcagct gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa catctgaccc
    77941 aatgtcatca tcccgctgat ttgtttatca gcctgctttt gcgctttttc cgcatccatt
    78001 ccttcagata aattaagctg ttttgcggtt tctgtgacga cattaagcgg taacttcaca
    78061 tcaaaattga gcgatttgat atctttgttt gtggatgatg aagatttggc tgggtcggca
    78121 atactgatat ccagattggc atttagttcg ccgagtgcgt tcttccatct taccggttgt
    78181 ttaatggtcg gctcactttt ttgtaataac ggtaaatact ctttgaacaa tgcagcattc
    78241 acttcttgca gagcaacttc atcgtttgct aactcagggt gtacagcaag ctgcttctgc
    78301 atagcaatgt tatactggat gataaattgg cgcactgctg agggatcaat agattcagca
    78361 atcaatgacc actcaccact accgagttgc tgattccctt ttgttaactt atcaagttcg
    78421 taggtcagat tgacattgat ataatctttg tctttaacgc gatccagcat ggtttttgca
    78481 acgaaagcat cgatttgggc aacgtcttcc ccgtgattga taatgttaat ctgattaaat
    78541 ttcgactcgc tttcaccaac cggaaatttc ttttcttcta atcgcgccag agaatcaatg
    78601 gtaaaacttt ttgccgttac tttgttcatt ggtgataatt gcagaacaat atcttcgact
    78661 ttgccttcaa tatccaggtt agacaatgaa ctaccatcac cattaaaagt aaactggccc
    78721 tcaccaaaag aaaactttcc atactctgca ggggcaacct ctaatgtcgt agagaatgag
    78781 ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc ggcgataaat
    78841 agcggttgtg tcagttcatt attcactaag ttcacttttg ccgctgccag tgctgggatg
    78901 acattaccat gcattaacat tgtgatgggc aacggaccgt gctcaacatc cacatcaaat
    78961 acaactttct ggcctggctt gatattgaga tcgggtgcac cgttatcgaa tgtcatttgc
    79021 atttgaaaac gggaattaaa aaagcctttg tcaaaatttt gataacttaa cgtcatatcg
    79081 taggcatgtt cacctttctt tttagcatcg ttaaaatctt taataaattt ttcgacacca
    79141 ggctgaattt gtgtacctgt gtaccatgtg ccaccacccc agattacggc taacgcaaca
    79201 ataacacctg tagctgactt acgtatcata taaatattcc ctgataaaac agcaaaactt
    79261 tgaatatcca cttatgctga aataatgt
//
LOCUS       DAZFYC010000008        70896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFYC010000008 DAZFYC010000000
VERSION     DAZFYC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.008"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(156..2570)
                     /gene="ybbP"
                     /locus_tag="ACHKW5_000679"
     CDS             complement(156..2570)
                     /gene="ybbP"
                     /locus_tag="ACHKW5_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO7510121.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLLLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHLELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(2567..3253)
                     /gene="ybbA"
                     /locus_tag="ACHKW5_000680"
     CDS             complement(2567..3253)
                     /gene="ybbA"
                     /locus_tag="ACHKW5_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO7510122.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLGHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            3224..3847
                     /gene="tesA"
                     /locus_tag="ACHKW5_000681"
     CDS             3224..3847
                     /gene="tesA"
                     /locus_tag="ACHKW5_000681"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO7510123.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            3837..4646
                     /gene="ybbO"
                     /locus_tag="ACHKW5_000682"
     CDS             3837..4646
                     /gene="ybbO"
                     /locus_tag="ACHKW5_000682"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7510124.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            4707..5561
                     /gene="cnoX"
                     /locus_tag="ACHKW5_000683"
     CDS             4707..5561
                     /gene="cnoX"
                     /locus_tag="ACHKW5_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO7510125.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5624..6403)
                     /gene="fetB"
                     /locus_tag="ACHKW5_000684"
     CDS             complement(5624..6403)
                     /gene="fetB"
                     /locus_tag="ACHKW5_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO7510126.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(6390..7067)
                     /gene="fetA"
                     /locus_tag="ACHKW5_000685"
     CDS             complement(6390..7067)
                     /gene="fetA"
                     /locus_tag="ACHKW5_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO7510127.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            7213..8130
                     /gene="qmcA"
                     /locus_tag="ACHKW5_000686"
     CDS             7213..8130
                     /gene="qmcA"
                     /locus_tag="ACHKW5_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7510128.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            8127..8585
                     /gene="ybbJ"
                     /locus_tag="ACHKW5_000687"
     CDS             8127..8585
                     /gene="ybbJ"
                     /locus_tag="ACHKW5_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO7510129.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(8586..8993)
                     /gene="cueR"
                     /locus_tag="ACHKW5_000688"
     CDS             complement(8586..8993)
                     /gene="cueR"
                     /locus_tag="ACHKW5_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO7510130.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(8990..9087)
                     /locus_tag="ACHKW5_000689"
                     /pseudo
     CDS             complement(8990..9087)
                     /locus_tag="ACHKW5_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9118..10410)
                     /gene="ybaT"
                     /locus_tag="ACHKW5_000690"
     CDS             complement(9118..10410)
                     /gene="ybaT"
                     /locus_tag="ACHKW5_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7510131.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(10413..11345)
                     /gene="glsA"
                     /locus_tag="ACHKW5_000691"
     CDS             complement(10413..11345)
                     /gene="glsA"
                     /locus_tag="ACHKW5_000691"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO7510132.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPQLDEEGNSVRGQKMVASVANQLGYNVFKG"
     gene            11607..14111
                     /gene="copA"
                     /locus_tag="ACHKW5_000692"
     CDS             11607..14111
                     /gene="copA"
                     /locus_tag="ACHKW5_000692"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO7510133.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(14326..14667)
                     /gene="ybaQ"
                     /locus_tag="ACHKW5_000693"
     CDS             complement(14326..14667)
                     /gene="ybaQ"
                     /locus_tag="ACHKW5_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7510134.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            14805..15599
                     /gene="ybaP"
                     /locus_tag="ACHKW5_000694"
     CDS             14805..15599
                     /gene="ybaP"
                     /locus_tag="ACHKW5_000694"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO7510135.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            15803..16282
                     /gene="ybaK"
                     /locus_tag="ACHKW5_000695"
     CDS             15803..16282
                     /gene="ybaK"
                     /locus_tag="ACHKW5_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO7510136.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(16319..17971)
                     /gene="ushA"
                     /locus_tag="ACHKW5_000696"
     CDS             complement(16319..17971)
                     /gene="ushA"
                     /locus_tag="ACHKW5_000696"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7510137.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            18189..19409
                     /gene="fsr"
                     /locus_tag="ACHKW5_000697"
     CDS             18189..19409
                     /gene="fsr"
                     /locus_tag="ACHKW5_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO7510138.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            19647..21323
                     /gene="ybaL"
                     /locus_tag="ACHKW5_000698"
     CDS             19647..21323
                     /gene="ybaL"
                     /locus_tag="ACHKW5_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO7510139.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGLVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(21456..22760)
                     /gene="gsk"
                     /locus_tag="ACHKW5_000699"
     CDS             complement(21456..22760)
                     /gene="gsk"
                     /locus_tag="ACHKW5_000699"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO7510140.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTLHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            22912..23871
                     /gene="aes"
                     /locus_tag="ACHKW5_000700"
     CDS             22912..23871
                     /gene="aes"
                     /locus_tag="ACHKW5_000700"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO7510141.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(23868..24830)
                     /gene="hemH"
                     /locus_tag="ACHKW5_000701"
     CDS             complement(23868..24830)
                     /gene="hemH"
                     /locus_tag="ACHKW5_000701"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO7510142.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGIGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(25066..25710)
                     /gene="adk"
                     /locus_tag="ACHKW5_000702"
     CDS             complement(25066..25710)
                     /gene="adk"
                     /locus_tag="ACHKW5_000702"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO7510143.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(25891..27765)
                     /gene="htpG"
                     /locus_tag="ACHKW5_000703"
     CDS             complement(25891..27765)
                     /gene="htpG"
                     /locus_tag="ACHKW5_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO7510144.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(27875..28480)
                     /gene="recR"
                     /locus_tag="ACHKW5_000704"
     CDS             complement(27875..28480)
                     /gene="recR"
                     /locus_tag="ACHKW5_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO7510145.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGLDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(28480..28809)
                     /gene="ybaB"
                     /locus_tag="ACHKW5_000705"
     CDS             complement(28480..28809)
                     /gene="ybaB"
                     /locus_tag="ACHKW5_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO7510146.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(28862..30793)
                     /gene="dnaX"
                     /locus_tag="ACHKW5_000706"
     CDS             complement(28862..30793)
                     /gene="dnaX"
                     /locus_tag="ACHKW5_000706"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO7510147.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(29465..29529)
                     /gene="dnaX"
                     /locus_tag="ACHKW5_000706"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(30922..31473)
                     /gene="apt"
                     /locus_tag="ACHKW5_000707"
     CDS             complement(30922..31473)
                     /gene="apt"
                     /locus_tag="ACHKW5_000707"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO7510148.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(31626..32003)
                     /gene="ybaN"
                     /locus_tag="ACHKW5_000708"
     CDS             complement(31626..32003)
                     /gene="ybaN"
                     /locus_tag="ACHKW5_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO7510149.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            32073..32600
                     /gene="priC"
                     /locus_tag="ACHKW5_000709"
     CDS             32073..32600
                     /gene="priC"
                     /locus_tag="ACHKW5_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO7510150.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            32614..32775
                     /gene="rsmS"
                     /locus_tag="ACHKW5_000710"
     CDS             32614..32775
                     /gene="rsmS"
                     /locus_tag="ACHKW5_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO7510151.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(32828..32926)
                     /locus_tag="ACHKW5_000711"
                     /pseudo
     CDS             complement(32828..32926)
                     /locus_tag="ACHKW5_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32987..36349)
                     /gene="mscK"
                     /locus_tag="ACHKW5_000712"
     CDS             complement(32987..36349)
                     /gene="mscK"
                     /locus_tag="ACHKW5_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO7510152.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(36477..37124)
                     /gene="acrR"
                     /locus_tag="ACHKW5_000713"
     CDS             complement(36477..37124)
                     /gene="acrR"
                     /locus_tag="ACHKW5_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO7510153.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            37266..38459
                     /gene="acrA"
                     /locus_tag="ACHKW5_000714"
     CDS             37266..38459
                     /gene="acrA"
                     /locus_tag="ACHKW5_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO7510154.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            38482..41631
                     /gene="acrB"
                     /locus_tag="ACHKW5_000715"
     CDS             38482..41631
                     /gene="acrB"
                     /locus_tag="ACHKW5_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO7510155.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            42178..42552
                     /gene="tomB"
                     /locus_tag="ACHKW5_000716"
     CDS             42178..42552
                     /gene="tomB"
                     /locus_tag="ACHKW5_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO7510156.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            42578..42796
                     /gene="hha"
                     /locus_tag="ACHKW5_000717"
     CDS             42578..42796
                     /gene="hha"
                     /locus_tag="ACHKW5_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO7510157.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            42968..43519
                     /gene="maa"
                     /locus_tag="ACHKW5_000718"
     CDS             42968..43519
                     /gene="maa"
                     /locus_tag="ACHKW5_000718"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO7510158.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            43635..44105
                     /gene="ylaC"
                     /locus_tag="ACHKW5_000719"
     CDS             43635..44105
                     /gene="ylaC"
                     /locus_tag="ACHKW5_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO7510159.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            44269..45819
                     /gene="pdeB"
                     /locus_tag="ACHKW5_000720"
     CDS             44269..45819
                     /gene="pdeB"
                     /locus_tag="ACHKW5_000720"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO7510160.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(45861..46214)
                     /gene="ybaA"
                     /locus_tag="ACHKW5_000721"
     CDS             complement(45861..46214)
                     /gene="ybaA"
                     /locus_tag="ACHKW5_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFO7510161.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(46334..46430)
                     /gene="ffs"
                     /locus_tag="ACHKW5_000722"
     ncRNA           complement(46334..46430)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKW5_000722"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            46593..46904
                     /locus_tag="ACHKW5_000723"
     CDS             46593..46904
                     /locus_tag="ACHKW5_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO7510162.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(46935..47507)
                     /gene="ybaY"
                     /locus_tag="ACHKW5_000724"
     CDS             complement(46935..47507)
                     /gene="ybaY"
                     /locus_tag="ACHKW5_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO7510163.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            47725..48585
                     /gene="tesB"
                     /locus_tag="ACHKW5_000725"
     CDS             47725..48585
                     /gene="tesB"
                     /locus_tag="ACHKW5_000725"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO7510164.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(48633..49919)
                     /gene="amtB"
                     /locus_tag="ACHKW5_000726"
     CDS             complement(48633..49919)
                     /gene="amtB"
                     /locus_tag="ACHKW5_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO7510165.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(49949..50287)
                     /gene="glnK"
                     /locus_tag="ACHKW5_000727"
     CDS             complement(49949..50287)
                     /gene="glnK"
                     /locus_tag="ACHKW5_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO7510166.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(50468..52249)
                     /gene="mdlB"
                     /locus_tag="ACHKW5_000728"
     CDS             complement(50468..52249)
                     /gene="mdlB"
                     /locus_tag="ACHKW5_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7510167.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALHQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(52242..54014)
                     /gene="mdlA"
                     /locus_tag="ACHKW5_000729"
     CDS             complement(52242..54014)
                     /gene="mdlA"
                     /locus_tag="ACHKW5_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7510168.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(54044..54502)
                     /gene="decR"
                     /locus_tag="ACHKW5_000730"
     CDS             complement(54044..54502)
                     /gene="decR"
                     /locus_tag="ACHKW5_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO7510169.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(54656..55474)
                     /gene="cof"
                     /locus_tag="ACHKW5_000731"
     CDS             complement(54656..55474)
                     /gene="cof"
                     /locus_tag="ACHKW5_000731"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO7510170.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            55574..57274
                     /gene="ybaE"
                     /locus_tag="ACHKW5_000732"
     CDS             55574..57274
                     /gene="ybaE"
                     /locus_tag="ACHKW5_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO7510171.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            57339..58034
                     /gene="queC"
                     /locus_tag="ACHKW5_000733"
     CDS             57339..58034
                     /gene="queC"
                     /locus_tag="ACHKW5_000733"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO7510172.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(58086..58484)
                     /gene="fadM"
                     /locus_tag="ACHKW5_000734"
     CDS             complement(58086..58484)
                     /gene="fadM"
                     /locus_tag="ACHKW5_000734"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO7510173.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLESEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(58578..58949)
                     /gene="ybaV"
                     /locus_tag="ACHKW5_000735"
     CDS             complement(58578..58949)
                     /gene="ybaV"
                     /locus_tag="ACHKW5_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO7510174.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(59100..60971)
                     /gene="ppiD"
                     /locus_tag="ACHKW5_000736"
     CDS             complement(59100..60971)
                     /gene="ppiD"
                     /locus_tag="ACHKW5_000736"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7510175.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNEGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(61163..61453)
                     /gene="hupB"
                     /locus_tag="ACHKW5_000737"
     CDS             complement(61163..61453)
                     /gene="hupB"
                     /locus_tag="ACHKW5_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO7510176.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(61644..63998)
                     /gene="lon"
                     /locus_tag="ACHKW5_000738"
     CDS             complement(61644..63998)
                     /gene="lon"
                     /locus_tag="ACHKW5_000738"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO7510177.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(64186..65460)
                     /gene="clpX"
                     /locus_tag="ACHKW5_000739"
     CDS             complement(64186..65460)
                     /gene="clpX"
                     /locus_tag="ACHKW5_000739"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO7510178.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(65586..66209)
                     /gene="clpP"
                     /locus_tag="ACHKW5_000740"
     CDS             complement(65586..66209)
                     /gene="clpP"
                     /locus_tag="ACHKW5_000740"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO7510179.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(66455..67753)
                     /gene="tig"
                     /locus_tag="ACHKW5_000741"
     CDS             complement(66455..67753)
                     /gene="tig"
                     /locus_tag="ACHKW5_000741"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO7510180.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(68097..68414)
                     /gene="bolA"
                     /locus_tag="ACHKW5_000742"
     CDS             complement(68097..68414)
                     /gene="bolA"
                     /locus_tag="ACHKW5_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO7510181.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            68719..69297
                     /gene="yajG"
                     /locus_tag="ACHKW5_000743"
     CDS             68719..69297
                     /gene="yajG"
                     /locus_tag="ACHKW5_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7510182.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            69341..70816
                     /gene="ampG"
                     /locus_tag="ACHKW5_000744"
     CDS             69341..70816
                     /gene="ampG"
                     /locus_tag="ACHKW5_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO7510183.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT        16668 a        18983 c        17993 g        17252 t
ORIGIN      
        1 ctatatttaa aattattaaa ccccaccgat tcagattaat atcgcaatag aaagatagga
       61 aatcaccaat tcaataacat catcaatgaa acatattgta gaattaattt aaactaggcg
      121 ttccattcaa ccagaacgcc ccttatttac tctcttcacc ccgcaaactg cctgaacagc
      181 gccttaccct taaccagtcg cgcacccagc cagccgccga aaagcgacag cagcagcgca
      241 ccgctgcacg gcagaacaat ccacaatcgc cagtctggct cccacggaaa atcaaacact
      301 ttcgcctgca acactgccag cgccgtttcc gcaccaattg cggctaccag gccagaaaca
      361 aacccaagca tggcgaactc acaccacaat gtcgtgcgca gcagcttttt ccccgcaccg
      421 agtgtgcgcc acaccaccag ctccagatga cgctgacgca ttcccacctg cacctgagcc
      481 agcaacagca acataccgca ggcggtgacc agtaccacca tcacttccag cgcccgactt
      541 acctgctcca gcacctgacc gacctgtttt aaaatcgcgc caatatctaa cagactaatg
      601 gtcgggaact ggcggttgag ttgtgtcaac atgccgttgc cattctccca gcggaaactg
      661 gtaagccagc tctgcggttg cccgtctaat gccccttcag ggaaaataaa atagaaatta
      721 ggccgcagac tttcccagtc cactttgcgc aggctggtca ctttagcgcg gaactcctgg
      781 gtatcgccca taaaagtcac ggtatcaccg agggcaacgt ttaagcgttt cgccagcccc
      841 tcttccatcg acacttcatc ggcttttggc ggccagttac cggcgacaat cggattatga
      901 tcgggccgcg tattttgcca ggtaagattg agttcgcggt taagcgcctc atcttcatta
      961 ccttctgtcg gcttatcgtt aatcgccgtc agccgcgccc gcaccaccgg ataaaacgat
     1021 tccgggacta tctgatgttc cgcgaggaac gcttttagcg gtgctacctg ttctgtggcg
     1081 atgttaatta aaaagtagtt cgggctttct ggaggtagct gctgctgcca gcggtcgagc
     1141 agatcaccac gcaacaccag cagcagtgcc aacagcataa aggagagcga aaatgccgaa
     1201 agctggctta acgttgacca cggctgacgt aacaggcggc taaccgccag gcgcagaggc
     1261 agcgatttca gcgtcatgcg gcgaagtaca ttcagcagca tccagcctag aacaccgcac
     1321 agcaaagcca gtactaccgc gcccgccagc accgcccaga gcagcatact gccgcccatt
     1381 aatccggcga gcagcagcac aaccaccaca ctgacaatcg gcagataaaa cttcagcggc
     1441 cagacgttcg ctaccacatc attacgtaat acgcgtaaag gctgcgttgc cagcaacaag
     1501 cggtacggtc gtagccccac cagcagcgag atgactgtca tggtgccgag cgcccacagc
     1561 cacggccaga ggctggctgg cggtagtgca gcaggcagaa cgggcttgag cagcaccatc
     1621 aacacgtttt cgaacaacag gcctatcgcc ccaccggtaa cggccgaaag caacagcacc
     1681 atcaaccact gaccgacgat tagcttacgc agttgcgctc gccctgcccc cagcgttttg
     1741 aggatcgcca ccagatcgta gcgactgcga cagtaatgat tcatcgctac cgccactgct
     1801 gccactgcca gcagcaaggt cagaagcgcc gaaagcagca ggaactgttg cgagcgttcc
     1861 atcgatcgcc ccagcgcgcc ttcgtcctgt tccagaccgt accagcgttg ttcgggttta
     1921 agctgaggta acaaccattt ctcatagccg tcgagctggt tctcactgcc gccgaattta
     1981 tagcgccagg tgacccgact ccccggctgc acggctccgg ttttatcgac atccgccaga
     2041 ttcatcatca gacgcggagc catctggaag gggttaaaac cggaatccgg ttcctgaatc
     2101 acttctccgg caatccgcaa ggtggcatcg cccacgtcaa tggtgtcgcc cgttttcagg
     2161 ttaagcagcg ccatcaggcg tggggccagc aatacgctgc ccgcctgcgg tttcaggcca
     2221 gggggattag tttgcagatc gccatacatc gggtagatat catccaccgc tttgacgttc
     2281 gccagctgcg gtgtgtcgcc tgcaaatgtc atggtggcga aagtcagctg cttgccgact
     2341 ttcaggccgc gcttttgcgc ttcctccagc catgcttgcg gcacttcgcg cgaactgcgc
     2401 aacgcccgat cgcccgccat aaactcccgg ctttgttggc ttaagccctt ctccatgcga
     2461 tcgctgatat tgcccagcgc cagcacgcag gccaccgcca ggcttagcgc cagccagaca
     2521 attaatagcg acggcgaacg ccattcgcgc cagaaccaac gtgcaatcat gcttcctcct
     2581 gcaactgccc gttcaccagc cgtaagcagc ggtcgcagcg tgccgccagt tgcaggtcgt
     2641 gggtcaccat aatcaacgtg gtgccatgtt cacggttgag ggaaaacagc aggtcggcaa
     2701 ttttatcgcc cgtctggcgg tcaaggttgc cggtgggttc gtcggcaaac agcacatcag
     2761 gtcgaccatt aaaggctcgt gccagcgcca ctcgttgctg ttcaccgccg gaaagctgtg
     2821 ccggaagatg acccagacgt ttacccagcc ctaactgttc gagcaacgct ttcgccccgt
     2881 tacgactttc cgcgctactc tcaccgcgca gcagagccgg aagctcgacg ttttccagcg
     2941 cgttaagggt aggaattaac ataaatgact gaaaaacaaa gccgacgtgc ttcgcgcgca
     3001 actttgcccg cgcttcttcg tccatattat gtagcggttg tcccaccaga ctcacttcgc
     3061 cactgctgcc gtcatcaagc ccggcgagga tcgccagcaa ggttgactta cccgatcccg
     3121 actcgcccac cagtgcgatg gtctcgccac gtttgacaac cagctcaact ccggtgagga
     3181 tggagagttc atgctccccc tgaccgacgg acttcttaag atgatgaact tcaacaatgt
     3241 tttccgctgg catttgccct tcctgttcct ggtcctgtta accttccgtg ccgccgcagc
     3301 ggacacgtta ttgattctgg gtgatagcct gagcgccggg tatcgaatgt ctgccagcgc
     3361 ggcctggcct gccttgttga atgataagtg gcagagtaaa acgtcggtag ttaatgccag
     3421 catcagcggc gacacctcgc aacaaggact ggcgcgcctt ccggctctgc tgaaacagca
     3481 tcagccgcgt tgggtgctgg ttgaactggg cggcaatgac ggtttgcgtg gttttcagcc
     3541 acagcaaacc gagcaaacgc tgcgccagat tttgcaggat gtcaaagccg ccaacgctga
     3601 accattgtta atgcaaatac gtctgcctgc aaactatggt cgccgttata atgaagcctt
     3661 tagcgccatt taccccaaac tcgccaaaga gtttgatgtt ccgctgctgc ccttttttat
     3721 ggaagaggtc tacctcaagc cacaatggat gcaggatgac ggtattcatc ccaaccgcga
     3781 cgcccagccg tttattgccg actggatggc gaagcagttg cagcctttag taaatcatga
     3841 ctcataaagc aacggagatc ctgacaggta aagttatgca aaaatcggtc ttaattaccg
     3901 gatgttccag tggaattggc ctggaaagcg cgctcgaatt aaaacgccag ggttttcatg
     3961 tgctggcagg ttgccgaaaa ccggatgatg ttgagcgtat gaacaacatg ggatttaccg
     4021 gcgtgttgat cgatctggat tcaccagaaa gtgttgatcg cgcagcagac gaggtaatcg
     4081 ccctgaccga taattgtctg tatgggatct ttaacaatgc cggattcggc atgtatggcc
     4141 ccctttccac catcagccgt gcgcagatgg aacagcagtt ttccgccaac tttttcggcg
     4201 cacaccagct caccatgcgc ctgttacccg cgatgttacc gcacggtgaa gggcgtattg
     4261 tgatgacatc atcggtgatg ggattaatct ccacgccggg tcgtggcgct tacgcggcca
     4321 gtaaatatgc gctggaggcg tggtcagacg cactgcgcat ggagctgcgc cacagcggaa
     4381 ttaaagtcag cctgatcgaa cccggtccca ttcgtactcg cttcaccgac aacgtcaacc
     4441 agacgcaaag tgataaacca gtcgaaaatc ccggcatcgc cgcccgcttt acgttgggac
     4501 cggaagcggt ggtggacaaa gtacgccatg cttttattag cgagaagccg aagatgcgct
     4561 atccggtaac gctggtgacc tgggcagtaa tggtgctcaa gcgcctgctg ccggggcgcg
     4621 tgatggacaa aatattgcag gggtgagttg aagcgcgcgc ttaagccccc atgtcaaaga
     4681 aaatatcgac aacagagagc gactccatgt ccgtagaaaa tattgtcaac attaacgaat
     4741 ctaacctgca acaggttctt gaacagtcga tgaccactcc ggtgctgttc tatttttggt
     4801 ctgaacgtag ccagcactgt ttgcagttaa ccccgatcct ggaaagcctc gcggcgcagt
     4861 acaacgggca atttattctg gcgaagctgg actgtgacgc ggagcagatg attgccgccc
     4921 agtttggtct gcgcgcgatt ccgaccgtgt atctgttcca gaacgggcaa ccggtagatg
     4981 gcttccaggg gccgcaaccg gaagaggcga tccgcgccct gctggataaa gtgctgccgc
     5041 gcgaagaaga gctgaaagcg cagcaggcga tgcaactgat gcaggaaggc aattacaccg
     5101 atgccctgcc attgctgaaa gacgcctggc agttgtcgaa tcagaacggg gagatcggcc
     5161 tgctgctggc agaaacgctg attgcgctga accgttctga agatgcggaa gcggtgctga
     5221 aaaccattcc gttgcaggat caggacaccc gctaccaggg gctggtggcg caaatcgaac
     5281 tgctgaagca ggcggctgat acgccggaaa ttcaacagtt gcaacagcag gtggcggaga
     5341 atccagaaga tgccgcactg gcgacgcaac tggctctgca actgcaccag gttgggcgca
     5401 atgaagaggc gctggagttg ctgttcgggc atctgcgtaa agatctcacc gccgcagacg
     5461 gtcagacacg taaaacgttc caggagatcc tcgctgcgct gggtacgggt gatgcactgg
     5521 cgtcgaagta tcgccgccag ctgtatgcat tgttgtattg atattccgtt gatcggcaca
     5581 tgaaccacaa cgccggatgc gatgctgcgc atccggcatc gcatcatttc ttcttcaatt
     5641 gcgtcaccac caactggtgg cgcgaattat aaaacttacg ataggttaaa tagcaggcaa
     5701 taatggtcga caagctggcg gttgagagca gcataaaggt caccataatc tgatatttaa
     5761 tcgccttcac cggatcaatc ccggcaaata tcagcccgga catcattcct ggtaaactca
     5821 ctaagccaac cgtttttgct gaatcgaccg tcggaattaa agccgcgcga atactgtcgc
     5881 gaatcaatat cgctgaagcc tgcttcggcg tcgcaccaag actcagtttc tcctgaatct
     5941 gttgctgttc gctaatgacc cgttgcccta aattgttgta acacaacccc accgctacca
     6001 tggcgttacc ggcaatcatc ccggcgatag ggatcacctg catcgggata aattcaatcg
     6061 acccagagag aatcagcacc gccagggtaa ttcccgcccc gaccgtaatg gcaataaacg
     6121 atgagataaa agctttagca atatatttac tgcgtttttg cgcgttccac gccgcattaa
     6181 agcagataaa taacaccatc aataatgtca ggctggcatc atccacgctg aaaatatact
     6241 tcagcacata gccgacaata atcagctgaa ttatcgctcg cccgacgctc cagaaaatat
     6301 ctttctccag cgccagtttt tctttatggc taattaagat tgccaccacc accagcatta
     6361 atgccagtgc caatgattcg ttagtaatat tatgcgagtt catagcgtgc ttcctgcatt
     6421 tctccggcat gcggttgcag tgtaatcact ttatccgcat gattaatttc gtctttatcg
     6481 tgtgtcaccc acagcacggc aatattttgc tcgcgcacat aacgatggat catctcatta
     6541 acgttatgtt tattactttc atccagcgca ctggtgattt catccagcaa taaaaccttc
     6601 ggcataaatt gcaggttacg aatcagtgag atgcgttgtt tttcaccacc agatagctcg
     6661 gcgatatttt tcgtcaaaat gctgtccggc aaagcgaagc gttcgagaaa atcgagaaaa
     6721 atggccgggt caggctgctg gttacggatc tgccagggaa agatcagatt atcgtatacc
     6781 gtatcgccaa acagcgtcgg cgtctgggcg cagtaagaga cttgctggcg gtagatttct
     6841 ggctttagtg tgctgacatc ctcaccttca aacagtaacg ttccgctggt tgggctgatc
     6901 aatgaagcaa ctatttttag cagcgtactt ttgccacaac cggaaggacc ggtaattaac
     6961 ttaaattcgc cagcacgcag cgaaaaattg atgttattaa gaatcttcgc atcacccgcc
     7021 agatatccta cgttttgtag ctgaagcaaa ggactatttt cctgcatcac tgttcccttt
     7081 ttctgatttt tactaaaaac agtttatcct tcgcaggaat aagggggaac tctctttcag
     7141 taatcaggta taatttgttt aatcaagcag caattttgct gattatggac ggtacaacag
     7201 gaggtttttc cgatgcttat ctttatcccg attctcattt ttgtcgcgct ggtcattgtc
     7261 ggcgcgggcg tcaaaatcgt gccgcagggc tatcaatgga cggtagaacg ttttggtcgc
     7321 tataccaaaa cgttacagcc ggggctcagt ctggtggtgc cgtttatgga tcgcattggt
     7381 cgcaagatca atatgatgga gcaagtgctc gatatccctt cccaggaagt tatctcgaaa
     7441 gataacgcca acgttaccat cgacgcagtc tgttttattc aggtgattga cgcgccgcgc
     7501 gcggcttatg aagtcagcaa tctggagctg gcgatcatca acctgaccat gactaacatc
     7561 cgtaccgtgt tgggttcaat ggaacttgac gaaatgctct ctcagcgcga cagcatcaac
     7621 tcacgcctgc tgcgtattgt cgatgaagcc accaacccgt gggggattaa agtcacccgt
     7681 attgaaattc gcgacgtgcg cccaccggca gagcttatct cttcaatgaa cgcgcagatg
     7741 aaagcggaac gtaccaaacg cgcttacatt cttgaagcgg aagggatccg tcaggcggaa
     7801 atcctcaaag ccgaaggtga aaaacagtcg caaatcctga aagcggaagg cgaacgtcag
     7861 tcggcgtttt tacaggctga agcgcgtgaa cgttccgccg aagcagaagc ccgcgccacc
     7921 aaaatggtgt ctgaagctat cgcctccggt gatattcagg cggtgaacta cttcgtagcg
     7981 cagaaataca ccgaagcgtt acagcagatc ggttcctcca gtaacagcaa agtagtgatg
     8041 atgccattag aggccagcag cctgatgggg tcgattgccg ggattgccga gctggtgaaa
     8101 gacagcgcca acaaacggac tcagccatga tggagttaat ggtcgttcat ccacatattt
     8161 tctggctcag tctcggcggt ttgctgctgg cagccgagat gctgggcgga aatggttatt
     8221 tgttgtggag cggcgtggca gcggtgatta ccggcctggt ggtctggctg gtgccgctgg
     8281 gttgggagtg gcaaggggta atgtttgcca tcctgacgct gctcgccgcc tggctgtggt
     8341 ggaaatggtt gtcgcggcgg gtgcgcgaac aaaagcacag cgatagtcat ttaaaccagc
     8401 gcgggcagca gctgattggc cgacgttttg tgctggaatc tccgctggtc aacgggcgcg
     8461 gtcatatgcg cgtcggtgac agttcatggc ctgtcagcgc cagcgaggat ctcggtgcag
     8521 gtacgcatgt tgaagtcatt gcgatagaag ggataaccct acatatccga gccgtctcgt
     8581 cttaatcacc ctgcccgatg atgacagcag ccggagagat tttcgataat cgggcagtcg
     8641 gcgctgtcat cgccagggca ggcattcgcc agtgccagca gctggtcgcg catggattgc
     8701 agctcctcaa tgtgtcgttc gatctccgcc accttctcca gcgtgcgccg tttgacgtcg
     8761 gcgctgtgcc gctgcgggtc gttaaacaga ttcaccagct cgccgctctc ttccaggtta
     8821 aagcccacct gccgtgcctg gcgcagtaag gtcagttcgt tgagatgctg ctgcgtgtag
     8881 gtgcgataac cgttttcgct gcgcatcggc ggcgtcacca gccccttctc ttcatagaag
     8941 cgaatggctt tgctggtcag gccggtaatt tttgctacat cgctgatgtt catcgttcgc
     9001 gcaacgcctc ctgtgctttg ttaaagggtt tcaacagata ttcaagcacg tttttttgcc
     9061 agtgcgaata tcgaccgcca ccacaataat acaggaagta ctgcgcacag agagatgtta
     9121 tacggtttta ttgcgcttca tgaccattgc cacaataagg ctgagtaaga gtgagccaat
     9181 aatccatatc aacgcacggc taccctggga ccacagactg tagataaagc caacgatcac
     9241 caacaacatc accaacgtac caacgataag aatcggcaac gaggcgtgaa tatcatgacg
     9301 caggcggatc gccaccacaa acaccgccag gtagcaaatc aaaaaggtgg cgctggcaac
     9361 actggcgagt gagcctaaat ttagcgccgc cgtcatcagc ataatcaaca ccacgacaat
     9421 gatgttgccc caggtactct gccgccacag ggatttattc attagcttcg gcagttcgcg
     9481 ttcgctgccc atgttgtcca tgatgttaaa cacggcgaac aggttcgcgt taatggctga
     9541 agccgtcgcc agtaaagcgc cgatgacgac gatcacataa cccacatgtc cgagcagcgg
     9601 agaagcagcc tgcgcaacgg cggtatcggc atatttttct aactctaatg ccgatacatc
     9661 gctaagcaaa accagtgcca gcgagatata aagcaacgtg gtaacgccaa tcgccaccag
     9721 aaacgcccgc ggcataatga cctgcggatc tttcacttta tccgccgcgt tcgccatcat
     9781 gccaaagccc gcataggcaa ggaaggttat cccgatacag gagaagaacg caccggagct
     9841 ggggggcgca gagacggaaa tatgcgccgg ttgcagcgac cagacaccgg caataatcag
     9901 caataacagg atcatcattt taatgccgac gaggatcact tccagccgcc ctaccgcatg
     9961 gttgcttaag gagttgaaaa gcgtcatcac cgcaatgatc cccaacgcgt agagcaaaat
    10021 aaggtgctcc tcctggctgc cttcatgcaa aaactgcacg gcataagcgc caaaagcacg
    10081 ggcgaccatg gcgatgctca ccgccagcgt caacaggtac agtaacgaga gcgccagcga
    10141 aaagacgccg ttgcctaatc cgcgacgaaa gaagtcgata atgccgccat tgctgggata
    10201 gctcgccccc agacgcgcat aggcataacc ggaaaacatc gccacaatac cgccaaaagc
    10261 aaaggcgacc caggtcgagg cttccattag caacgcagcc tgccccagca gcgcgaagat
    10321 ccccgccccc accattgccc caataccgat ggaaacgaca ttccatagac cgaggggttt
    10381 gttaccgtta ttaccttccg tgttcatcat gatcagccct taaacacgtt atagccgagt
    10441 tgattagcga ccgatgccac cattttttga ccgcgaacac tgttgccttc ttcgtccagc
    10501 tgtggtgaga acgcagcaat tcccatcacg ccagggacga ccgccagaat accgccacct
    10561 acaccgcttt tgcccggtaa accaacgcga tacgcccagt caccggagcg accatacagc
    10621 ccttccatca tcatttcggc cagaatgtac ggcacgttgt cggcctgaag aacgcgttca
    10681 tgcgtcagcg gattcacacc ccccgccgcc agcgtcgcgc caagtgttgc cagttcaaca
    10741 gtattgagga gcgtggagca ctgacgggtg tacacgtcac aggcttccat tgcatcacaa
    10801 tagagatatc cggcggagta cagcagccag gcaatggccc ggttatggaa gttggttgtt
    10861 tgttccgact ggttgacttc gtcagagagc gctacctgct caccagccag ttgctgttgg
    10921 atatgtaaaa ttcgctgcca gcgttgttca acattttcag cgttaatcag gctggtggtg
    10981 gcaatggcgc cagcatttac cagtggcgac agtggtttgc cgccatgcaa ctctaaggcg
    11041 ataactgagt taaagggcaa tccggtcggg tcagcgccaa ttttgtcctg taccgcctgc
    11101 gggccgacat cttctaacgc aagggctaac gtacagactt tcgagatgga ttccagcgca
    11161 aagcggtaat cactatcacc cgcgcgatag atgttgccat cgcaggtcac aatagccact
    11221 gctgccagtt gactaggtac attcgccaga aagggaatgt agtcggcatt ttgtccgccg
    11281 ttaagtgagt gaaattgggt gtaagcctga tccactgcct gctgtaattt gtttgcatct
    11341 aacatctttt gttaactcct ttttatagat gcgggaggta attcctcacc ccggtgccga
    11401 ttttcaggca tcttgattta acttagcacc cgcaacttaa ctacaggaaa acaaagagat
    11461 aaatgtctaa tcctgatgca aattgagcca attttttaat ctttacggac ttttactcgc
    11521 ctggtttatt aatttcttga ccttcccctt gctggaaggt ttaaccttta tcacagccag
    11581 tcaaaactgt cttaaaggag tgttttatgt cacaaactat cgacctgacc ctggacggcc
    11641 tgtcctgcgg tcactgcgtt aaacgcgtga aagaaagtct cgaacagcgc ccggatgtgg
    11701 aacaagcgga tgtgtctatc actgaagcgc acgttaccgg gactgccagt gcagaacagc
    11761 tgatcgaaac catcaaacaa gcgggttatg acgcatctgt aagccaccca aaggctaaac
    11821 cgctggcgga gtcatcaatc ccgtcggaag cactgacagc ggtttctgag gcgcttccgg
    11881 cagcgaccgc cgatgacgat gacagccagc agttgctgct gagcggcatg agctgcgcca
    11941 gctgtgtcac ccgcgtacaa aatgcgctgc aaagcgtacc gggcgtcact caggcacggg
    12001 taaacctggc ggagcgtact gcgctggtga tgggcagtgc ctccccacaa gatttagtgc
    12061 aggcggtgga aaaagcgggc tacggcgcgg aagcgattga agatgacgcg aaacgccgtg
    12121 agcgccagca ggaaaccgcc gttgctacga tgaagcgctt ccgctggcag gcaattgtcg
    12181 cactggcggt gggtatcccg gtgatggtct gggggatgat tggcgataac atgatggtca
    12241 ccgctgacaa ccgcagcctg tggttggtta tcggcctgat aaccctggcg gtgatggttt
    12301 tcgccggcgg ccatttttac cgcagtgcat ggaaaagcct gctgaacggt gcagcgacga
    12361 tggatacgct ggtggcgctg ggtactggcg tggcgtggct ctattcgatg agcgtcaacc
    12421 tgtggccgca gtggttcccg atggaagcgc gacatcttta ttacgaagcc agcgcgatga
    12481 ttatcggtct gatcaatctc ggccatatgc tggaagcacg cgcacgtcag cgttcttcta
    12541 aggcgctgga gaaattactc gatttaaccc caccgacggc gcgcctggtt actgacgaag
    12601 gtgaaaaaag cgtgcctctg gcggaagtgc agccaggtat gttgctgcgc ctgacgaccg
    12661 gcgatcgcgt gccggtagat ggcgagatta cccagggcga agcatggctg gatgaagcga
    12721 tgctgacggg cgaaccaatc ccgcagcaaa aaggcgaagg cgaaagcgtc catgccggga
    12781 cagtggtaca ggacggtagc gtgctgtttc gtgccagtgc ggttggcagc cataccacgc
    12841 tgtcacggat cattcgcatg gtgcgccagg cccagagcag caagccagaa atcggtcagc
    12901 tggcggataa aatctcagcc gtatttgtac ctgtggtggt ggtgattgcg ctggtcagtg
    12961 cggcaatctg gtacttcttt ggtccggcac cgcaaattgt ttataccctg gtgattgcca
    13021 ccacggtact gattatcgcc tgtccgtgtg cgctggggct ggcgacgccg atgtcgatta
    13081 tttccggcgt cgggcgggcg gctgagtttg gcgtgctggt gcgggacgcc gacgcgctgc
    13141 aacgcgccag tacactcgat accgtcgtgt tcgataaaac cgggacgctg accgaaggga
    13201 agccgcaggt tgtcgcagtg aaaacgtttg ctgatgttga tgaagcgcag gcattgcgtc
    13261 tggcggcggc actggagcaa ggttccagcc atccgctggc acgggcgatc ctcgataaag
    13321 caggtgatat gcagctgccg caggtcaacg gtttccgcac attgcgcggg ctgggcgtga
    13381 gcggtgaagc tgaaggtcat gcgttattgc tgggcaatca ggcgctgtta aatgagcaac
    13441 aggttggtac caaagctatc gaagcggaga ttactgctca ggcatcgcaa ggggcaacgc
    13501 ctgtgctgct ggcggttgac gggaaagcgg tagccctgct ggcagtacgc gatccattgc
    13561 gtagtgatag cgtggcggcg ctgcaacgcc tgcataaagc gggatatcgt ctggtgatgt
    13621 tgaccgggga taacccaacc actgccaatg cgatcgccaa agaagccggg attgatgaag
    13681 tgatcgccgg ggtgctgccg gacggtaaag ccgaagcgat taaacgcctg caaagtgaag
    13741 gacgtcaggt ggcaatggtg ggcgacggca ttaacgacgc gccagcgctg gcccaggcgg
    13801 atgtcggcat tgcgatgggt ggcggcagtg atgttgccat tgaaaccgcg gcgattaccc
    13861 tgatgcgcca tagcctgatg ggcgttgcgg atgcgctcgc gatttcccgc gcaacgctgc
    13921 gcaacatgaa gcagaacctg ctcggtgcct ttatctacaa cagcatcggt attccggtcg
    13981 ccgccggtat tttgtggccg tttactggaa cactgcttaa cccggtagtt gccggagcgg
    14041 caatggcgct ctcgtcgatt accgtagtga gtaacgccaa ccggttgctg cggtttaaac
    14101 cgaaggaata agtacgtcat tgcggatgcg ctgctttagg gcggcgcatt tggcatcgac
    14161 gcatgactgc cggatgcggc gtaaacgcct tatccggcct acggttcagg cacaggtttg
    14221 taggcctgat aagacgggta agcgtcgcat caggcaatgg tgctcggatg ccggatgcgg
    14281 cgtgaacgcc ttatccggcc tacgagatga agcgatgttt tggccttacg cgaccttttt
    14341 tgcacgctct tcacggcgtg ccaaatattc agccactgtt tcaatccgtc ccaattcttc
    14401 ctgggtgcgc atgttgtttt caacttccca aagatcaact gccgcctgga ggtttagcct
    14461 aaaatcgact gtggtatcaa aaactttcgc cagacggaat gccatctcag tggtgagttt
    14521 acgattgtta ttgatcagtg cactgacgct attacgatga acatgcagca actctgctaa
    14581 ctcattgatt ttcaaatcga gcggttccag atattcatag agaagaatat ctccgggtgt
    14641 cgtcggtttt cttgttgcct gtttcatggt ttttaactgt tgcttccctg tgaatagcga
    14701 tgcaagaaca tactgaatca tacaaacaca ttaaacaagt ccccaactcc atttgcgcca
    14761 tccctcaccg cgcgctagca tgatatttca caaagggagg tcgtatggat ctgttgtacc
    14821 gggtaaaaac gctttgggcc gcgctgcgcg gtaatcatta cacctggcct gccatcgata
    14881 tcacccttcc cggcaatcgc cattttcatc tgattggcag tattcatatg ggtagccacg
    14941 atatggctcc cctgcccacc cgtttgctca aaaagctcaa aaacgccgat gcgctgatcg
    15001 tcgaggcgga tgtttccacc agcgatacgc cttttgctaa tttgcctact tgcgaggcgc
    15061 tggaagagcg cattaacgag gagcaactcc aaaatctgca gcacattagc caggagatgg
    15121 gcatttctcc gtcgctcttt tctactcaac cgctgtggca aatcgcgatg gttcttcagg
    15181 cgacgcaggc acaaaaactg gggctgcggg cagaatacgg tatcgattac cagttattgc
    15241 aggcggcgaa gcaacaacat aaacccgtga ttgaactgga aggggctgaa aaccagattg
    15301 ccatgttgct ccagctccct gacaaaggac tggcgctgct ggacgatacg ctgacccact
    15361 ggcataccaa cgcacggttg ctgcaacaaa tgatgagctg gtggctgaat gcaccgccgc
    15421 aaaataatga tataacgctg cccaatacgt tcagtcagtc gctgtacgat gtgctgatgc
    15481 atcagcgaaa tctcgcctgg cgggataaat tacgcgccat gccgccgggg cgatatgtgg
    15541 tcgcggtcgg tgcactacac ctgtatggag aagggaattt gccgcaaatg ttgcgctaaa
    15601 aaaatggcca atatcgctat tggcccgtca aagaggaatt tcattttttt attattatgc
    15661 cgtcacttta agcgacggtg taactcgatt gtcgctcaat ctcaccatcc tgccaatact
    15721 cttaggcaag atgactcttt tttttggacc gccatcaggc gtatgcttgc ttcgtttttt
    15781 tgtttaaggc aggaaaatta ccatgacacc cgcagttaaa ttactcgaaa aaaacaagat
    15841 ttcgtttcaa atccatacct acgagcacga tccggctgaa accaattttg gcgatgaagt
    15901 cgtcaaaaaa ttaggtttga atccggatca ggtctacaaa acgctgctgg tggcagtgaa
    15961 cggtgatatg aaacaccttg ccgtggccgt tacgccggtc gcaggtcaac tggatcttaa
    16021 aaaagtagca aaagcgctgg gtgccaagaa agttgagatg gccgatccga tggtcgcgca
    16081 gcgttcgacg ggatacctgg ttggggggat tagcccactg gggcagaaaa aacgtctgcc
    16141 aacgattatc gacgcccccg cacaagaatt tgccactatt tatgtttccg gcggcaagcg
    16201 cggactggat atcgaactgg cggcaggcga tctggcaaag atcctcgatg ccaaatttgc
    16261 tgatatcgcc cgccgcgatt aagcattgtg ccggatgcaa acatccggca ctttcggatt
    16321 actgccagct cacctcacct ttcggttcat aaacactcac atccagcggc gagcttttct
    16381 ggatatacgc tttcagcact tcggcatcaa taaagccggt attcacatag cccggtttgt
    16441 tatcaaggcg cggatatcca tcaccgccgg tggcattgaa gtttaatgtc gccatacggt
    16501 aagttttcgc cggatcgacc ggttcgcctt tgattttaag gtcgttcagt ttgccgtctt
    16561 tcgccacaaa gctaacgttg gcaaattgcg ggtaggcacc tgaatctggc ttcatctgcg
    16621 cgacggcggt caggtaatca atcacctctt taccggtcat gtcggcatac accaccacat
    16681 tgccgaatgg ctgcactttc agcacgtttt tatagctgat atcgcctgct tcgatagaat
    16741 cacgaattcc gcctccgctc atcaccgcaa agtcggcacc agtgcgatcc atttgggctg
    16801 ccagaatcaa ccgccccata ttggtctgta caaaacgcac tttgtcacga tcgccttcca
    16861 gacgaccatt ggtttcgcct attttcactt ccagctgcgc tttgcctttg ttctggaacg
    16921 gtgataacag cgagatcatt tgctggtttt cagcgatttc aggagtgtaa agcacgcgct
    16981 cgcttttccc gtcttcccag gtcactttct tcttcaggtt caccggaatc agctggtagt
    17041 taaccatttt catttcgcca ttacgaaact caaaatcagc ccgtcccacg tatttgcccc
    17101 actcatgcgc ctgcacaatc cagatgccgt tttgttgatc tggcttgcat ggcgtacccg
    17161 gcacgtaatc gacctgtttt ttgttttctg ccgccatgca gaccggatct tgcgagtgac
    17221 caccgacgat catcgccagc gatccggcag gcagcgcgcg tgccatctcc acatcgcccg
    17281 gtgcgttaga gccgtgctca ccattatcgt aatgccccat atgggtcgcc gcgataataa
    17341 tgtctggctt ttctgtctgt tgcagctcct gaatcaccag cttcgcttca tcggcgggct
    17401 tacgaaattc gacatcagtg aagtattccg ggttaccaat ttttgctgtg tcatcggttg
    17461 tcagcccaat gacggcaatt ttcagatcct gacgcttaaa cagcgcccac ggtttaaaca
    17521 ggcgctcgcc agtacttttc tggtagatat tcgcggaaag caacgggaac ttggcccact
    17581 tttcctgctg gcgtaatacg gtgagcggat tatcaaattc atgattaccg atcgccatcg
    17641 cgtcatagcc caccagattc ataccgcgaa aatcaggttc ggcatcctgt aagtcagact
    17701 cgggcacgcc agtgttaatg tcgccaccgg aaagtagcag cacgctaccg ccttcagccg
    17761 caacctcttt gcggatacca tccaccagcg ttttttgcgc cgccagacca tattcgccat
    17821 attcattgcg ccaaaaatgc ccatgatgat cattggtatg cagaactgta attttgtagg
    17881 ttttatcctg ctcatacgcc agagcagttt cactcgccag tgtaaatgtg gttaacagcg
    17941 ctaacgccac gccccgctgc aataatttca tacttctctc cctgacctga tttcaacgtg
    18001 ttgaaaaata gtatgacgcg acatcataga caagtctgcg tccggtgaag ataaatctgc
    18061 atccagatta gaaattctgt attcggcgtt gagacttcct tcaggcgacg catacaggta
    18121 tgttagtcgg gtaataatca caaataagat cattctttac atcgcaaccc tgaaaaagtg
    18181 actttcctat ggcaatgagt gaacaacccc agcctgtggc gggcgcggct gcgtcaacga
    18241 ccaaggcccg aacatcgttt ggtattttag gtgctatcag cctctcacat ctgctgaacg
    18301 acatgatcca atcgctgatt ctggcgattt atccgctgct tcagtcagaa ttttctctga
    18361 catttatgca gattggcatg ataaccctca ccttccagct cgcctcttcg ctactgcaac
    18421 cagtggtcgg ctactggacc gataaatatc cgatgccgtg gtcgttgcca attggcatgt
    18481 gctttacctt aagtggtctg gtgctgcttg cgctggcggg cagttttggc gcagttctgc
    18541 tggcggcggc gctggtcggt accggttcat cggtctttca tccggaatct tctcgcgtag
    18601 cccgtatggc ttccggcggg cggcatggcc tggcgcaatc tatctttcag gtcggcggca
    18661 actttggcag ttccctggga cccttgctgg cggcggtgat tatcgcgcct tatggcaaag
    18721 gcaacgttgc ctggtttgtg cttgcggcac tgctggcgat cgtggtgttg gcgcaaatta
    18781 gccgttggta ctcggcacag caccgaatga ataaaggaaa atccaaagcg acgataatca
    18841 atccactgcc gcgcaacaaa gtggtactgg cagtcagcat tctgttaatc ctcattttct
    18901 cgaaatattt ctatatggcg agcatcagca gctattacac cttttatctg atgcaaaaat
    18961 tcggattatc tatccagaat gcccagcttc atctgtttgc cttcctgttt gccgttgcgg
    19021 caggtacggt gatcggcggg cctgtagggg ataaaattgg gcggaaatat gtgatttggg
    19081 gctctatcct cggcgttgcg ccgtttacgc tgattttacc ctacgccagc ctgcactgga
    19141 cgggggtttt aacggtgatt attggattta tcctcgcttc ggcattctct gccattctgg
    19201 tctacgctca ggagctactt ccgggacgta tcggtatggt ttctggactc tttttcggtt
    19261 ttgcttttgg catgggaggt ctgggagcgg cagttctggg gcttatcgcc gatcacacca
    19321 gcatcgagtt agtctataaa atctgtgctt tcctgccact attggggatg ttgaccatat
    19381 tcctgcctga taaccggcat aaagactgat ttcattgcag ccaaaggcaa actttggctg
    19441 catcgtttta cagtcgccat aagcctttcc tctgttaaac cgccttctgt tttccgatcc
    19501 gtgctttttt tgcagctaat tccatttttc tgcaaaattc aataattatt catgaacatc
    19561 atcaatgaaa ttgtcataaa ctattacctg taattttggt tttcccggcc aaaaatggac
    19621 aataaccacc aggaaaagga gacggaatgc atcacgccac cccgcttatc accaccattg
    19681 ttggcggcct tgtgctcgcc tttatcctcg gcatgctggc caataaacta cgtatttctc
    19741 ctctggtggg atatctgtta gcgggtgtgc tggcaggacc attcactccg ggctttgttg
    19801 ccgataccaa gcttgcgccg gaactggctg aactgggcgt cattctgttg atgtttggcg
    19861 tcggtttgca cttttcgctg aaggatttga tggcggtaaa ggccatcgcc attcccggtg
    19921 cgatcgccca gatagccgtg gcgacgctgc tgggtatggc gctctctgcc gtgctgggct
    19981 ggtcgttaat gaccggtctc gtgttcggtt tatgtctttc caccgccagt accgtggtgt
    20041 tactgcgcgc acttgaagaa cggcaattaa ttgacagtca gcgtgggcaa atcgccatcg
    20101 gttggttgat tgtggaagac ctggtaatgg ttctgacgct ggtgttgctg cccgcagtgg
    20161 caggaatgat ggaacagggc gatgtgggct ttgccactct tgcagtcgat atggggatca
    20221 ccatcggcaa agtgatcgca tttatcgcca ttatgatgct ggtaggtcgc cgtctggtgc
    20281 cgtggattat ggcacgcagc gcggcaaccg gttctcgcga gctgtttacc ctgtcggtgc
    20341 tggcgctggc gttagggatt gcctttggtg cggtagagct gtttgatgtc tcctttgcac
    20401 tcggtgcgtt ctttgccggg atggtactga acgagtctga actgagtcac cgtgccgccc
    20461 acgatacgct gccattgcgc gacgcgtttg cggtgctgtt ttttgtctcc gtcgggatgt
    20521 tgtttgatcc gttaattctg attcagcaac cgctggcagt gctggcgacg ctggcgatta
    20581 ttctgtttgg taagtcgtta gccgcatttt tcctggtgcg actgtttggt cactcccaac
    20641 gtacggcatt aaccatcgcc gccagcctgg cgcagattgg tgagttcgcg tttatcctgg
    20701 cgggactggg aatggcattg aatttactgc cgcaggccgg acaaaacctg gtactggcag
    20761 gggcgatcct gtcgattatg ctcaacccgg tactgttcgc actactggag aaatatctgg
    20821 cgaagaccga aacgctggaa gagcagacgc tggaagaggc aatcgaagaa gagaaacaga
    20881 tcccagtgga tatttgcaac catgcgctat tggtgggtta cggtcgtgta ggcagcctgc
    20941 tgggggagaa attgctcgcc tctgatattc cgctggtggt gattgagacg tcacgaaccc
    21001 gtgttgatga gctgcgagag cgtggggtcc gcgctgtatt gggcaatgca gcgaacgaag
    21061 aaattatgca actggcgcat ctggaatgtg caaaatggct gatcctgacg atccccaacg
    21121 gttatgaagc gggtgagatt gtggcgtctg cccgcgcgaa aaacccggat attgagatta
    21181 tcgcccgcgc ccattatgac gatgaagtga cgtatatcac cgaacgtggc gcgaatcagg
    21241 tagtgatggg tgaacgtgaa attgcccgta ctatgctgga actgctggaa acgccaccgg
    21301 caggtgaagt ggtgacgggg taaacggtgg ttgccggatg taaaatatga aaaatatgta
    21361 ggcatgataa gacgcgccag cgtcgcatcg ggcatcgaga gccaaatgcc agatgcggcg
    21421 tgaacgcctt atctggccta cgaaccgacg ataacttaac gatcccagta agactcttcc
    21481 aggctgtctt cacgctccgg caagccgcgc gttaaacgag gtgaatgctg gttcagtacc
    21541 tgatagctca cacggttagc atatttacac acctgcgcta acgatgaata agttaaccag
    21601 gtgaatttat gtttgctgga gtttggtacg ttgctacgat ggtagctgtt ggcggtaatg
    21661 tcatgcagca acgctgccaa tgcgccatcc cccgctccat tagtgttcat gattttttcc
    21721 ggcccgccca tgtacggcgc aatgtgcgaa tatacacgca gcggattctg gcaatccttg
    21781 tggcgcatgg cgcggctaaa ctcatactgg ttgaattccg ctatagcgcc cggcagcagc
    21841 ggatgcaggg ttttacgttt cgcttcgtct tcggtaaagc ccgccatata caagccgatt
    21901 ggcccggcgg tgcacagcac cagatctacc cagtccagcg ccttgtcaga tgccaacaac
    21961 ggatcgcttt ctccggtcaa cgcttcggct tcatcttcgt tcatcgcaag gatagaaacg
    22021 tggtctttga ggaattgctg ccaccactgc ggattctcgg caatgacaaa cttggtgccc
    22081 agcgtcagca ccaccggtac gttatatttc ttcgcgtact caatggcttt catggttgct
    22141 tccggcatgg gttcacccgg tttgcaacgc accagataag aggtgagaac cagtgccgag
    22201 gctccggcaa tcacatcttc cggaatgctt tcagcccgca gctggttcat gtggcctgga
    22261 ctgatagcaa aggtacgttc cccggactcg ccaatcagcg taaagcaacg accaatcggg
    22321 ccatccacgc cttgtagata gttaagatcg gtacggctgg aagtgttaca caggtaacga
    22381 taggcataac tgccaatttc aatattgctg cacatgacgc ccagcagcac cgaacggtcg
    22441 tccgcgagca ccgagtagtt gtgcatggtg ttaccaatgg tgccacccgc aaactgatgg
    22501 gtaatcaggt ttttctgttt tagctcctga taaagcgctt cggctacgtc atcctcaatc
    22561 accagtgaat gcccggcgct taatccataa cgctcaatga attcatcatc cactttcgct
    22621 tcaatatcga ccagcgtctg gtcaatacct acgacccagg cggcgctggt ttcgttttct
    22681 ggctggaatt gctgaagcag cggatcgcgt gcatttacag ggaagtaatg tttggattta
    22741 cgtttaccgg gaaatttcat ggttgttttt acgggtggtt aactgagcgg cgaatgttaa
    22801 cacaatctga cgggagcgcg cgagacgcag ccagtttcaa tatcagcagg cgggatcgca
    22861 ttttcatggc agtagttcct tacaatgacg taatttgaaa ggagtttttg tatgaagccg
    22921 gaaaacaaac tacctgttct ggaccttatt tctgctgaaa tgaagaccgt tgtgaatact
    22981 cttcagccgg atttaccgtc ctggcccgca acgggaacga ttgctgagca acgacagtat
    23041 tacacgcttg agcgccgatt ctggaatgcg ggcgctccag aaatggcaac cagagcttac
    23101 atggttccaa caaaatatgg gcaggtggaa acacgtctct tttgtccgca gccagatagc
    23161 ccggcgacgc tattttattt gcatggaggc ggttttattc tcggcaatct cgatacccac
    23221 gatcgcatca tgcgcctgct ggcaagctac agccaatgta cggtgattgg tattgattac
    23281 cccctttcac ctgaagcgcg ttttccgcaa gcgatagagg aaattgtggc tgcttgttgt
    23341 tatttccacc agcaggcgga ggattatcaa atcaatatgt cccgcattgg ctttgccggt
    23401 gattccgcag gtgccatgct ggcgctcgcc agtgcgttgt ggttgcgtga taaacagatc
    23461 gattgcggta aaattgcggg tgttttgctg tggtatgggc tttacggatt acgggattcc
    23521 gtgactcgtc gtctgttggg cggtgtctgg gatggcttaa cgcaacagga tttgcagatg
    23581 tatgaagagg cgtatttaag caacgacgcg gaccgcgagt caccgtatta ctgtctgttt
    23641 aataatgatc tcactcgcga agttccgccc tgttttattg ccggggcgga gttcgatccg
    23701 ctgctggatg acagccgtct gctttaccag acgttagcgg cgcatcagca gccctgtgag
    23761 ttcaaactct acccaggtac gctgcatgcc tttttgcatt attcacgaat gatgaaaacc
    23821 gccgacgagg ctcttcgcga cggcgctcag ttctttaccg ctcagcttta gcgatacgcg
    23881 gcaacaagat tagccatcat ttcgatatgt tccggcgtgg cattaagcgc tggaatatat
    23941 tcatattttt tcccgccggc accgaggaag acctcacggt tttgctcggc aatctcttcc
    24001 agcgtctcca gacaatccgc agcaaagccc gggcacatca cctgtatatg acctatgcct
    24061 ttttctccga gcattttcag cgtttcgtcg gtataaggca tcagccaggg ttcccggcca
    24121 aagcgcgact gaaaggtcat catcactttt tccggtgcca tccccagtgc ggaagccagt
    24181 tcgcgagtcg ttgtgcggca acgttgcggg taatcatcgc cttcatctgc ataacgctgg
    24241 ggaatgccat gataagagag cagcagcaga tccggttcgc catgtttggc aaaagaagcg
    24301 cgtacgctgt tcgccagtgc attaatgtaa tcgtggttat cggcgtaatc acgaataaac
    24361 gatatccccg gaatgctacg tttgcgcgcc agaatgcgtg ccagttcatc ccataccgca
    24421 ccgaccgtag aacaggagaa ttgcggataa agcggcagca ccacaatatg atctacatgc
    24481 tctgccagga gttcatcgac ggcgctttcc agtgatggcg agccgtagct catgcccagc
    24541 gctacgggcg tctccggtaa acgttgtgcc agcgcctgct gttgctggcg gctgtaaacc
    24601 atcagcggcg agccgtcttc catccagacc gaggcgtaca gcttcgccac acgcggcgag
    24661 cgcagcggca aaatcacgcc gcgcagcaac ggccaccata acaaccgtga ggtatcaacc
    24721 acgcgtctgt cgcttaaaaa ttgtttcaga tagcgtttta ccgcttcagg tgtgggggca
    24781 tcgggcgtac ccaggtttgc cagcaggata ccggttttag tctgacgcat taccgcctct
    24841 tatcgattca acttgttgat aattgtagcg gaaaagtgag gaagaagaac taattgctgt
    24901 gagaggtcaa atcagaaaaa ggaatagcgc agattgaggt ttttgtaaaa ggccgaatga
    24961 atatccggcc tgagattgct gataagtttg cttgtgcggg cctgttcccc tcaccctaac
    25021 cctctccccg agggggcgag gggactgtcc gtgcgcgctt tcgaattagc cgaggatttt
    25081 ttccagatca gcgcgaactt cagcaaccgg cttggtgccg tcaactttcg cgtatttggt
    25141 gttacccgct tccgcttctt tggagtagta gccgatcagc ggtgcagtca tctgatggta
    25201 ttcaaccaga cgtttacgca cggtttcttc ctgatcgtct ttacgggtag tcagttcttc
    25261 accggtaacg tcgtctttgc cttctacttt cggcggattg aatttaacgt gataaacacg
    25321 accagacggc gcatgaacgc ggcggcctac gatacgatca acaatcagtt cgtccggtac
    25381 gtcgaattcc agaacgtaat caacattgat gcccgcttct ttcatcgcgt ctgcctgcgg
    25441 aatggtacgc gggaagccgt ccaacaggaa accattacgg cagtcttcct gagcaatgcg
    25501 ctctttaacc agcgcgatca ccagttcgtc ggtgaccagt ttgccagcat ccataatgtc
    25561 ttttgcttgt ttacccagct cggagccaga tttgaccgca gcacgcagca tatcgccagt
    25621 ggagatttgc ggaataccat atttctccat gatgaactga gcctgagtcc ctttccccgc
    25681 gcccggagcg ccaagcagaa tgatacgcat tgcgaaaatc cccttaaaat gtgtcgaatt
    25741 ttttgaaaaa gcgataaacg ataccaccaa caggcgattg cctcaagaaa ggcacctgag
    25801 gctgaaacgg ttaatggcga gataatttgc ggaaagtgca gatgatgaaa agaaaaatgc
    25861 cggatgacac aaaggtcatc cggcatgaca tcaggaaacc agcagctggt tcatacgacg
    25921 aataaacagg ttcggatctt ccagcgtgcc gcgttctgcc agcagcgcct gatccagcag
    25981 cagttctacc cactcgctga acttcgcttc atcttcagta tctgccgcac gtttcaccag
    26041 tacgtgatcc gggttcagtt cgaagatgta tttcacttct ggcactttct ggcccgccgc
    26101 agcgaacagt ttcgccatct gagtgctcat ttcgtccgcg tcggtcgaaa cgatcgctgg
    26161 cgtatcggtc agacggtgag tcagacggac atctttcacg cgctcgccga gcagggcttt
    26221 cacacggtcg atgaacggag tcagtgcttt ctccgcttct ttcgcgctct catcaacttc
    26281 gtcagccagt ttttccagcg actcgtcaac tttagacacc gactggaacg gtttaccgtc
    26341 gaactcagtc agatagttca tcatccactc atcgatgcgg tcggaaagca gcagaacttc
    26401 gatgcctttc ttacgcagca gttccaggtg cgggctgctc ttcgctgccg catagctgtc
    26461 tgcagtgatg tagtagattt tctcctgccc ttctttcatg cgggaaacgt agtcttccag
    26521 agacacggtc tgcgcagaag aatcggtata agtagaagca aaacgcagca gtttggcgat
    26581 cgcttcctgg ttagcgaaat cttccgccgg accttctttc agtaccaggc caaactgttg
    26641 ccagaaggtc tggtatttgt ccgcgtcgtc tttcgccagt ttttccagca tttgcagcac
    26701 acgcttggtc agcgcattgc gcaggttacg cgttaccgtg ctgtcctgga ggatttcacg
    26761 ggaaacgttc agcggcagat cgctggagtc aatcagacca cgcacgaagc gcagatagtt
    26821 cggcatgaac tgttctgcgt cgtccatgat gaacacacgc tggacgtaca gtttcaggcc
    26881 gtgtttatga tcacggttcc acatatccca cggagcctgg gacgggatgt acagcaggct
    26941 ggtgtactcc tgcttacctt caacgcggtt gtggctccag gtcagcggat cattaaagtc
    27001 gtgggcgatg tgtttgtaga actctttgta ctcttcatcg gtgatttccg acttgttacg
    27061 agtccacagc gcctgcgctt tgttgatttt ctcccaggag ataacggttt cgccgtcttt
    27121 ctcttcgcgt ttttcgatct ctaccggcag cgcgatatgg tcggagtatt tgctgatgat
    27181 ggaacgcacg cgccagtcat cgaggaactc gtcttcgcct tcacgcagat gcagggtgat
    27241 ttcagtacca cgatcttctt tggtgatgtc ggcaacggta tattcacctt cgccagccga
    27301 ttcccagaag acgccgtttt ctggtttttc gcctgccgcg cgagtacgca cggtcacttt
    27361 gtcggccacg ataaacgcag agtagaaacc aacaccaaac tgaccgatca gctggctgtc
    27421 tttcgcctgg tcagaaccca gggattcgag gaatgatttg gtaccggatt tagcgatagt
    27481 ccccagatgg tcaatcactt cgtcgcgggt catccccacg ccgttatcgg agatggtcag
    27541 cgtgcgcttg tctttatcga aagagacacg aacgcgcagt tcgccatcac cttcgtacag
    27601 gtccgggtta gatagcgcac ggaaacgcag cttgtccgcc gcatcggagg cgttagagat
    27661 aagctcacgc aggaagattt ctttattgga atagagagaa tggatcatca ggtgcagaag
    27721 ctgtttcact tctgactgaa aaccacgagt ttcttgtcct ttcatgtagg tctacctcaa
    27781 taatgccatt ttaaggttaa aaacaggatg agggagagat ggggacaagg gagaataatt
    27841 tcaagcgaga gcaggtgatc ctgctctcgt ttgcttaaaa acgaatctta tgacgcccgg
    27901 caagggagtg tgacagcgtg gtgccatcga ccatttccag ttcgccgcca accggtacgc
    27961 catgagcgat tcggctggct tccacgtcat attgcgcgca aagctcggca atgtagttag
    28021 cggtagcttc accttcaacc gtgggattgg tggcgaggat cacttcagtg attttttcct
    28081 ctgccagacg ctgttccaga cgatcaaggc cgatatcatc cagaccgatg ccgtccagcg
    28141 gtgacaggtg ccccatcaac acaaaataac ggcctgaaaa ctgccccgtc tgctcaatgg
    28201 cgtagatgtc cgccggactc tccaccacac agatttgacc gttttcctga cgacgcggat
    28261 tcgaacagat gttacagact tcctgttcgg tgaaagtgcg gcaatcggcg cagtggccga
    28321 tttccgacat cgcccgggtg agcgcctgcg ccagacgcat cccgccgcta cgatcgcgct
    28381 gaagcagcgt gaacgccata cgctgcgccg acttcgggcc aacgcccggc agacagcgca
    28441 gtgcttccat aagctgtgtt aacagcgggc tggtttgcat cagaacggca tcttaaagcc
    28501 aggcggcagc tgcattccgg aggatacaga ggccattttt tctttctgcg tttcttcaat
    28561 acgacgtgct gcgtcgttga atgctgcagc caccaggtct tccagcatct ctttgtcgtc
    28621 ttccagcagg ctcgggtcga tctctacgcg acggcagttg tgtgcaccgt tgatggtcac
    28681 ttttaccaga cctgcgccag attcgccggt gacttccagc tgcgcgatct cttcctgcat
    28741 tttctgcatt ttttcttgca tctgctgggc ttgcttcatc aggttaccca gaccgccttt
    28801 accaaacata ggtttctctc tcaatcacgt taaggatgac gaacgtaagc tgtgcttacg
    28861 atcaaatggg gcggatactt tcttcatcca gctccgcatc gaagaaccga cgcagggtct
    28921 gaatattatt atccgcaata atggactcgc gcgcctgcgc aagtttttct tcgtatatcg
    28981 cctgacgcca ctccagcggc gtacgcaccg cgggattatc atcttcaacg atagtcagtt
    29041 caaccgttga accttttaac gtgctcaacg cttcagccag tttttgctgt gcaccgcggt
    29101 tgttcaaatg ccgctgagag gagcgcaaat gcagacatac tgcgttgtcg ctctcctctt
    29161 tccaggcatt taaggccacc tgttcgacca gttttggtag cgaaagttga ctcacctgtg
    29221 ccgcccacgc gtcgcgctca atggcttctg ccgccagctt cgccgccagt tccggcgttt
    29281 tttcatgttc cagcgctttt ttcagcgcct tcggcgtggc gaccacttct ttttgctgca
    29341 tcactggagt ggtagccttc cagcgatacg cttctttttt ggctggcgct ttttccagcg
    29401 ccgatggcac cggacgcgcc tgaacgcgat cggtgaccga agccagtctt tccagcgcag
    29461 cgttattcac cggccgcgcg cgggtagcgg ctgccggttc actctttttt gctttggttg
    29521 ctccctgcac gcgctgcaac tgctggcgcg ccgccagcac ctggctggtg gtttccggga
    29581 gcggtacagt cggtgcctgc tgcggcgctg attgcggttg cggcggcacc tgggttggcg
    29641 tcattactgc cgttggcgcg acgggcgcaa aggactggcg tggcacttct ggctcaggca
    29701 gaggcatacg tggatggaac gccagcgcgc gcagcagcgt catctcaacg cccatgcggc
    29761 ggtccggcgc atacggtaat tctttgcgac caatcaacag cgtctgatag taaagctgaa
    29821 tatccgtcgg cggtatggtg cgcgccagtt cacgcatccg cagctcaatg gcggccatgt
    29881 cgttgccaag tgcagcaggc gaaagttgta ccatcgcaat acgatgcaac aggccaagca
    29941 tttccaccag caacgcttcc cactcgatac cacgggcagc ggcttcatta atcagcgcca
    30001 ttacgcgctc gccgttggcc tcgaccatcg cttcaaccag cgacagcgcc tgatcgtcgt
    30061 caagcgtacc cagcatcgca ctgactgcct gggttgaaac ctggccgtca ccgctggcaa
    30121 tcgcctggtc ggtcagactt aaggcatctc gcaggctgcc ttcagcggcg cgcgccagca
    30181 attgcaacgc ccgcggctcg tgagcgatat gttcttcgtt gaggatgtgc tcaagttgat
    30241 ggcgaatttg ctcgacatcc agcgccttga gatgaaattg caaacagcgc gacaaaatcg
    30301 tcaccggcaa tttctgtgga tcggtcgtcg ccagcagaaa cttaacgtgc tccggcggct
    30361 cttcaagggt ttttaacagt gcgttaaagc tgtggcgcga cagcatatgc acttcgtcga
    30421 tcagataaac tttgaaacga ccacgcgccg gagcgtactg gacgttatcc agcaggtcgc
    30481 gggtatcttc aactttggtg cgcgaggcgg cgtcgatttc aatcagatcg acaaagcgcc
    30541 cctgctcgat ttcacgacag ttatcgcaca cgccgcacgg cgtcgcggta atgccggttt
    30601 cgcagtttag ccccttcgcc agcagtcggg cgatagaggt ttttccgacg ccacgggtac
    30661 cggaaaaaag ataagcatga tgaatacgcc ctaacgacaa gccgttcgcc agtgcggtca
    30721 gcacatgttc ctggccgacg acgtcagcaa aggtttgtgg gcgccatttt cgggctaaga
    30781 cctgataact cattggcagg ctctgaaacg ctggaaggtg gattcacgaa ggggtaatgc
    30841 taacacagcc ccgtcagaac ggcgaggctg aatgcacatc ttgtgctgtc cgtagcgtgg
    30901 gcagcacaag actggcgata attaatggcc cgggaacggg acaaggctgt agctggtaat
    30961 gccctgtttt tcgagacgct gttcgccgcc gagatcgaac aggttgataa tgaacgcagc
    31021 gtcagccact tcaccaccca gacgacggat cagtttaacg gtcgcttcga tagtgccgcc
    31081 ggttgccagc agatcgtcca ccaccagaac tttgtcgccc ggtttgatgg catcaacgtg
    31141 gatctccagc tgatcggtgc cgtattccag gtcgtaagtt tcactgatgg tttcacgcgg
    31201 cagtttgccc ggtttacgga ccggtacaaa gccaacgccc agacccagag ctaccggagc
    31261 gccaaacaag aagccacgcg cttcggtgcc gacaactttg gtaatgcccg catttttgta
    31321 acgctcaacc agcaagtcga tgctgagagc gtaagctttc gggtcttcca gtaagctggt
    31381 gacatcgcgg aaaagaatgc cgggttttgg gtagtcctga atgcttttga tgctattttt
    31441 gagatactca agctgctgtg cagtcgcggt cataagtgta tgcctgcttg ttacggtggt
    31501 actcacggcg cgttttaaac gtatcaaaaa taacagttgt ttaacctttg accgcctgcg
    31561 cctgtgctcg aaaacgccag aatgtactgg ctgtacgcaa taattgcaac tgcgattgtt
    31621 gtgcttcagt gcttttcttg cttttcatca atcaccggaa ttcgccacat atagaaaagc
    31681 aaacaggcga gaattaccag caacatgatg cgcacccatg gcatctggac gaaccacaga
    31741 gaaatggcaa acgtgagcaa aataagcaaa atcgcccgcg gtttgacgcc gcgcggcatc
    31801 gcatgatgtt tctgccagaa acgtagatag ctgccaaacc atgagcggta cagcaaccag
    31861 gcgtgaaagc gcggggaaga acgggcaaag caccaggccg ccagcaggat aaacggcgtc
    31921 gtcggtaata ccggtaatac cacgcccagc gtacccagca ctaccgccag ccagccaatg
    31981 atgattaaaa tgattcgttg catattgagt gttgtcagct tacagagtgg ctactttagc
    32041 ataacaatta tcattttcat tgaggtctta tcgtgaaaac cgccctgctc ctggaaaaac
    32101 tggaaggaca gctcgctacg ctgcgtcagc gttgtgcccc ggtagcgcaa tttgccacgc
    32161 taagtgcccg ttttgacaga cacctttttc aaacccgtgc gacaacactg caggcttgtc
    32221 tcgatgaggc gggcgataat ctggctgcgc ttcgtcatgc agttgagcag caacagctgc
    32281 cgcaagtggc ctggctggcg gaacatctgg cggcacaact ggaagccatc gcgcgtgaag
    32341 cctccgcctg gtcattgcgc gagtgggaca gtgcgccacc gaaaattgcc cgctggcagc
    32401 gtaaacgtat tcagcatcag gattttgagc ggcggctacg tgagatggtt gccgaacgca
    32461 gagcccgcct ggcgcgggtg accgatctcg tggaacagca aacgctgcat cgtgaagtgg
    32521 aagcctatga agcgcgcctg gcacgctgcc gccatgcgct ggaaaaaatc gaaaacaggc
    32581 tagcgcgctt aacccgctag caatggagag agtatgtcac tggaaaatgc ccctgacgat
    32641 gtcaaactgg ctgtcgattt gattgtgctg ctggaagaaa atcagatccc agcccgcacc
    32701 gtgctgcgcg cgctggatat tgtaaaacgc gattatgaaa agaaattaac gcgcgatgat
    32761 gaggcggaaa agtgagaaat aaatggggcc gctaaatgtc ccctaagatt gtgggcaaac
    32821 gcaaaactgc ctgatgcgct acgcttatca ggcctacacc gctcctgcaa tacattgaat
    32881 tcttgcggtt tcgtaggccg gataaggcgg tcacgccgca tccggcatga acaacgcgca
    32941 ctttgtcagt aatctcgggg caactaaatg tcgccccctt tggttattac cctaccgctg
    33001 gcgtcgggtc atcgcctttg tagtcgcgtt tgacttccgt cacctcatcg cccttctcgt
    33061 tgtgcagatg cacttcaagc tggttaaagg caatgttgat gtcgttttca cggcacagct
    33121 gatcgatagt acggttcagc tcatcgacag tacgactacg gtcacgcagt tcacgcacat
    33181 acagacgcag ctcatgatcc aacgtgctgg caccaaacgc cgtaaagaag acttccggca
    33241 ttggttcgtg catcaccctt gggtgctcag tcgccgcctt cagtaacact ttacgcactt
    33301 tttccagatc ggagccatag gccacgccga gacggatcac cagacgcgta gtagtgtcag
    33361 tcaacgacca gttgatcaga cgctcggtaa caaacgcttt gttcgggatg atcacttctt
    33421 tgcgatcgaa atcggtaatc gttgtcgcac gaatacggat cttacttacc gtccccgaga
    33481 agctaccaat ggttaccgta tcgccaatac gcaccggacg ttcgaacaga atgatcaaac
    33541 cggagacgaa gttaccgaaa atttcttgta aaccaaaacc aagacctacg gataatgctg
    33601 cggccagcca ctggagttta tcccaagaga cgcccagcga tccgaacacc gtcatcgcac
    33661 caacggcaat aatgatgtag ttaaggatgg tagtaatggc atacgacgcg ccctgacgca
    33721 tattcagtcg cgagagcacc agcacttcca gtaaaccagg caggttgcga atcaacgccc
    33781 aggccaccat tgaggcgata atcgcaaaca acagactgcc catggtgacg tttttcacca
    33841 ccgcagcgcc agcttcagtg ccgttgtaat gccagagcgt gatgctgtcg agatagctga
    33901 acacggtgat caaatcggac caaattgccc agaacatgac accgaacagc gcaaacatca
    33961 gcaacatggt aatacgcagc gtctgctggt taacctgttc cagtgcaatg gtgggttctt
    34021 ccggcggttc ggcaccttct gcgccctctt tcaccagatt ctgccgacgc gccagcgcac
    34081 gacgccaggc gatacgccgc gccgctacgc ttaagccgcg caataccgtc tggtagagca
    34141 agttccagat gatcaccaga taaacggttt caatccaacg cccggagagg cgcagcgtgg
    34201 tgtagaaata acccgtggca gtcagcacca tcaatgcaat cgggataatc gacagcacgg
    34261 taatggtgac cagtcgcatg gtgtgcgact ctttatcacg ccagctttcg cggcacattg
    34321 gccataccag gaaggcaatc agcagcaggt tgaagaaaat catcgcctgc cccagcacat
    34381 catccatcag atgcagcggg gaaagttctg ccaccacaga ccagaaatgg ataggcagca
    34441 acgcgagact gatgcggaca atttgccgac gccagtggct ggtctgctgc tccggcatgc
    34501 cgaagtgacg cacggcaacg ccatttttct ccagtacctt ccagcacagg ccaaacacca
    34561 gccagaaaat cgccagtttt ttactgaacg accatagcag ttcgctgatg ttgagctgca
    34621 tggtcaacag aatcaggcca accgcgagaa taatcaggca caccggcagc gcgcggatca
    34681 ggtcgataag gatcgctttt ggtgtgttga gctggctatc gttacgcagg gaacccacag
    34741 cggaagccag tttctgttga tacgctttca gccagcccag acgccagtgg atcagcccgg
    34801 caatcaacag cagcggcaaa ccggcgagga aagcgataaa aacggcaggc caggcttttt
    34861 cccagttcac cgtgattttc atcgacttaa attcatcttt caggctttgc gggaacgctt
    34921 tgatccagtc ccagtccatt ggacggttac tgttcaccca aaagatttgc tgagtcagga
    34981 tggatttcag gtttttcgat acgctcatta actgctgctg gttgatttgc aggttaatgg
    35041 ccatcatcag ctggttaccc aactgtttgt tgagttgatc cagcaattcg cgacgcatat
    35101 caaccacttg caataacgca tcgtgaactt cgctgttgac ttcgttggtg tgaccttctt
    35161 ccagtttgtt gacgaacgca tcgctctgga agagtgcatc acgctgctgg ttaacttcaa
    35221 actgttcgag acgcaaatcc gcgatgcggt tggtcatgtt ttccagttca tccgccgagg
    35281 gcagcgtttg ttgttgctgg taaaggatac gagacaacag caggctgccc ttcaggacgg
    35341 caatctgctc tttaatattg cgttccgatt gcagcgcccg ctccagccag tttttgactt
    35401 taatgttttg ctgcatcaac tgattaccgt tttcagtcgc ggtaatcaga cgctgactta
    35461 actgctggtt aatttccagt tcctgtttca ccagcggatt agcctgaata cgcgcggctt
    35521 catccgggga gacggcttcc tgcgccgttt tttcagttaa agtcaggcgc ttgctgttta
    35581 ccgcttcttg caacagctgc aactggtgct ccagacgggc gctgttcgcc gtcacgtaat
    35641 cacgttgctt ttgcaaggta tcctgcaaga cggtgttccc ttccaggctt ttacgctgct
    35701 ggtcaatctc cgcattcagc aacgcctgct gagcctgcat taacactttc tggctgggac
    35761 gtaaggcagt ctcgccgaca tcagtcccat ccagacgact gcgaatttgt tgcagctgct
    35821 gcgaagcgtt atacatcgca ttttgcacgc gttcgggctg cgtctgtaac gaaaccagct
    35881 ggctgttata agacgccaga tcgttttgtg cgttttgcaa atcgtccagc gcctgggcaa
    35941 cgcgagtttc cagctggcgc aacgaaagcg tactcagaat tttgcgcgtt tcttcgtcgt
    36001 tatcgacatc gctaagtgct gttaacgccg cggtcgcctg gcgcattttt tccggcgctt
    36061 cagcgacttt ttgccgtagc tgaactgtct cttcttttat gcgatcgatt ttatcgaggg
    36121 tggctaatgt atctgtcaga tcctgctgca ccagtttgtc ctgagcagaa agatcttttt
    36181 gtttatttag tgagtcaagt tgcgcctgca ggtccgcttt tgtcggcaga tcaccattcg
    36241 atgacgcccg cgcaaacgcc gtgttctggc ataacaagac aaaaacaaaa gcaataaatg
    36301 ctgaaaaaac aaaatgccgt gatcgtttgt aatactggaa catagtcatg atgaatgaag
    36361 gtttctgaac ctgaagaacg acctgaaaaa gtcaaaccgc aagaatatca cgacgcagtg
    36421 aaccagaata gcaacgacga aaatgtccag gaaaaatcct ggagtcagat tcagggttat
    36481 tcgttagtgg caggattacg aagcgtgggg cacaggagat acatctccag taagatggcg
    36541 acgtaatcgc gggcttcttt tttaagatca aaagattgcg gggcaaagag ccagttttcc
    36601 atcaggccgg aaatatagcc gcgcataata attgctgccc gacgcgtcat taaatccgca
    36661 ggcaacattt tcgcttcaat acaatgtttt aacgtttgtt ctatacggtc ataactttcc
    36721 agacagagat tacgttgtgc ctgttgcaca acagccattt ctccgacaaa ttcgcatttg
    36781 tggaatataa tctccatcaa taatcgacgc cgttcttctg tcaccgtgga ttcaagaaca
    36841 tgaattaata tctctcttaa tactgagagt ggatcgccag ggaattttgc ctgatactca
    36901 agctctagtt caccaatatt ggattctgac agttcccaga tctcactgaa caaatccgac
    36961 ttgtctttaa aatgccagta gattgcaccg cgcgtaacgc cagctgcttt tgcaatctcg
    37021 cccagcgagg tggatgatac cccctgctgt gagaaaagac gtagagccac atcgaggatg
    37081 tgttggcgcg tttcttgcgc ttcttgtttg gtttttcgtg ccatatgttc gtgaatttac
    37141 aggcgttaga tttacataca tttgtgaatg tatgtaccat agcacgacga taatataaac
    37201 gcagcaatgg gtttattaac ttttgaccat tgaccaattt gaaatcggac actcgaggtt
    37261 tacatatgaa caaaaacaga gggtttacgc ctctggcggt cgttctgatg ctctcaggca
    37321 gcttagccct aacaggatgt gacgacaaac aggcccaaca aggtggccag cagatgcccg
    37381 ccgttggcgt agtaacagtc aaaactgaac ctctgcagat cacaaccgag cttccgggtc
    37441 gcaccagtgc ctaccggatc gcagaagttc gtcctcaagt tagcgggatt atcctgaagc
    37501 gtaatttcaa agaaggtagc gacatcgaag ctggtgtctc tctctatcag attgatcctg
    37561 cgacctatca ggcgacatac gacagtgcga aaggtgatct ggcgaaagcc caggctgcag
    37621 ccaatatcgc gcaattgacg gtgaatcgtt atcagaaact gctcggtact cagtacatca
    37681 gtaagcaaga gtacgatcag gctctggctg atgcgcaaca ggcgaatgct gcggtaactg
    37741 cggcgaaagc tgccgttgaa actgcgcgga tcaatctggc ttacaccaaa gtcacctctc
    37801 cgattagcgg tcgcattggt aagtcgaacg tgacggaagg cgcattggta cagaacggtc
    37861 aggcgactgc gctggcaacc gtgcagcaac ttgatccgat ctacgttgat gtgacccagt
    37921 ccagcaacga cttcctgcgc ctgaaacagg aactggcgaa tggcacgctg aaacaagaga
    37981 acggcaaagc caaagtgtcg ctgatcacca gtgacggcat taagttcccg caggacggta
    38041 cgctggaatt ctctgacgtt accgttgatc agaccactgg gtctatcacc ctacgcgcta
    38101 tcttcccgaa cccggatcac actctgctgc cgggtatgtt cgtgcgcgca cgtctggaag
    38161 aagggcttaa tccaaacgct attttagtcc cgcaacaggg cgtaacccgt acgccgcgtg
    38221 gtgatgccac cgtactggta gttggcgcgg atgacaaagt ggaaacccgt ccgatcgttg
    38281 caagccaggc tattggcgat aagtggctgg tgacagaagg tctgaaagca ggcgatcgcg
    38341 tagtaataag tgggctgcag aaagtgcgtc ctggtgtcca ggtaaaagca caagaagtta
    38401 ccgctgataa taaccagcaa gccgcaagcg gtgctcagcc tgaacagtcc aagtcttaac
    38461 ttaaacagga gccgttaaga catgcctaat ttctttatcg atcgcccgat ttttgcgtgg
    38521 gtgatcgcca ttatcatcat gttggcaggg gggctggcga tcctcaaact gccggtggcg
    38581 caatatccta cgattgcacc gccggcagta acgatctccg cctcctaccc cggcgctgat
    38641 gcgaaaacag tgcaggacac ggtgacacag gttatcgaac agaatatgaa cggtatcgat
    38701 aacctgatgt acatgtcctc taacagtgac tccacgggta ccgtgcagat caccctgacc
    38761 tttgagtctg gtactgatgc ggacatcgcg caggttcagg tgcagaacaa actgcagctg
    38821 gcgatgccgt tgctgccgca agaagttcag cagcaagggg tgagcgttga gaaatcatcc
    38881 agcagcttcc tgatggttgt cggcgttatc aacaccgatg gcaccatgac gcaggaggat
    38941 atctctgact acgtagcggc gaatatgaaa gatgccatca gccgtacgtc gggcgtgggt
    39001 gatgttcagt tgttcggttc acagtacgcg atgcgtatct ggatgaaccc gaatgagctg
    39061 aacaaattcc agctaacgcc ggttgatgtc attaccgcca tcaaagcgca gaacgcccag
    39121 gttgcggcgg gtcagctcgg tggtacgccg ccggtgaaag gccaacagct taacgcctct
    39181 attattgctc agacgcgtct gacctctact gaagagttcg gcaaaatcct gctgaaagtg
    39241 aatcaagatg gttcccgcgt actgctgcgt gatgtggcga agattgagct gggtggtgag
    39301 aactacgaca tcatcgcaga gtttaacggc caaccggctt ccggtctggg gatcaagctg
    39361 gcgaccggtg caaacgcgct ggataccgct gcggcaatcc gtgctgaact ggcgaagatg
    39421 gaaccgttct tcccgtcggg tctgaaaatt gtttacccgt acgacaccac gccgttcgtg
    39481 aaaatctcta ttcacgaagt ggttaaaacg ctggtcgaag cgatcatcct cgtgttcctg
    39541 gttatgtatc tgttcctgca gaacttccgc gcgacgttga ttccgaccat tgccgtaccg
    39601 gtggtattgc tcgggacctt tgccgtcctt gccgcctttg gcttctcgat aaacacgcta
    39661 acaatgttcg ggatggtgct cgctatcggc ttgttggtgg atgacgctat cgttgtggta
    39721 gaaaacgtgg agcgtgttat ggcggaagaa ggtttgccgc caaaagaagc tacccgtaag
    39781 tcgatggggc agattcaggg cgctctggtc ggtatcgcga tggtactgtc ggcggtattc
    39841 gtaccgatgg ccttctttgg cggttctact ggtgctatct atcgtcagtt ctctattacc
    39901 attgtttcag caatggcgct gtcggtactg gtagcgttga tcctgactcc ggctctttgt
    39961 gccaccatgc tgaaaccgat tgccaaaggc gatcacgggg aaggtaaaaa aggcttcttc
    40021 ggctggttta accgcatgtt cgagaagagc acgcaccact acaccgacag cgtaggcggt
    40081 attctgcgca gtacggggcg ttacctggtg ctgtatctga tcatcgtggt cggcatggcc
    40141 tatctgttcg tgcgtctgcc aagctccttc ttgccagatg aggaccaggg cgtatttatg
    40201 accatggttc agctgccagc aggtgcaacg caggaacgta cgcagaaagt gctcaatgag
    40261 gtaacgcatt actatctgac caaagaaaag aacaacgttg agtcggtgtt cgccgttaac
    40321 ggcttcggct ttgcgggacg tggtcagaat accggtattg cgttcgtttc cttgaaggac
    40381 tgggccgatc gtccgggcga agaaaacaaa gttgaagcga ttaccatgcg tgcaacacgc
    40441 gctttctcgc aaatcaaaga tgcgatggtt ttcgccttta acctgcccgc aatcgtggaa
    40501 ctgggtactg caaccggctt tgactttgag ctgattgacc aggctggcct tggtcacgaa
    40561 aaactgactc aggcgcgtaa ccagctgctt gcagaagcag cgaagcaccc tgatatgctg
    40621 accagcgtac gtccaaacgg tctggaagat accccgcagt ttaagattga tatcgaccag
    40681 gaaaaagcgc aggcgctggg tgtttctatc aacgacatta acaccactct gggcgctgca
    40741 tggggcggta gctatgtgaa cgactttatc gaccgcggtc gtgtgaagaa agtttacgtc
    40801 atgtcagaag cgaaataccg tatgctgccg gatgatatcg gcgactggta tgttcgtgct
    40861 gctgatggtc agatggtgcc gttctcggca ttctcctctt ctcgttggga gtacggttcg
    40921 ccgcgtctgg aacgttacaa tggcctgcca tccatggaaa tcttaggcca ggcggcaccg
    40981 ggtaaaagta ccggtgaagc aatggagcta atggaacaac tggcgagcaa actgcctacc
    41041 ggtgttggct atgactggac ggggatgtcc tatcaggaac gcctctccgg caaccaggca
    41101 ccttcactgt acgcgatttc gttgattgtc gtattcctgt gtctggcggc gctgtacgag
    41161 agctggtcga ttccgttctc cgttatgctg gtcgttccgc tgggggttat cggtgcgttg
    41221 ctggctgcca ccttccgtgg cctgaccaat gacgtttact tccaggtagg cctgctcaca
    41281 accattgggt tgtcggcgaa gaacgcgata cttatcgtcg aattcgccaa agacttgatg
    41341 gataaagaag gtaaaggtct gattgaagcg acgcttgatg cagtgcggat gcgtttacgt
    41401 ccgatcctga tgacctcgct ggcgtttatc ctcggcgtta tgccgctggt tatcagtact
    41461 ggtgctggtt ccggcgcgca gaacgcagta ggtaccggtg taatgggcgg gatggtgacc
    41521 gcaacggtac tggcaatctt cttcgttccg gtattctttg ttgtggttcg ccgccgcttt
    41581 agccgcaaga atgaagatat cgagcacagc catactgtcg atcatcattg atacaacgtg
    41641 taatcactaa ggccgcgcaa gcggcctttt ttatgcataa cctacgaaac attaaggagt
    41701 aattgaacca ccaactcagg atctcatacg aaaaccagta ttaaccacgg ataaaattca
    41761 taaaaaatac tgattgttag ttaatttata ttaagtagcg ctaatagatt taataatcca
    41821 taatcattta gaggctattc ttaattattt gcggtaattc tttattcatt cctcggttat
    41881 tacgtcatat tcagagcaat cctggtatta gtgtcaccaa tttcatctgg cgataatcct
    41941 gaaatgttat gaacagttcg agcaaactgc ttttacctgc tgcgggttag tgctagtatg
    42001 aaaaagtgag tcctgtcccg attccttcct aattgtaatt tttcgtaata atgcgatgaa
    42061 aacatgcaaa gagtggctta tagttaagct aacaaacgag agggcaagtc caggtcagta
    42121 agttttttcc atcccgaaag gtgtccgtta gttcaaccac taagaagggg acgcgttatg
    42181 gatgaatact cacccaaaag acatgatatc gcacagctta agtttctctg tgaaaccctg
    42241 tatcatgact gtcttgcaaa ccttgaagaa agcaatcatg gctgggttaa cgacccaacc
    42301 tcggcgatca acctccagtt gaatgaactg attgagcata ttgcgacctt cgcacttaat
    42361 tacaaaatta agtataatga agacaataag ctcattgagc agatcgacga atatctggat
    42421 gacaccttta tgttgttcag cagttatggt attaatatgc aggatcttca gaaatggcgg
    42481 aagtcaggta atcgactatt ccgttgtttt gtcaatgcga cgaaagagaa tcctgcgagt
    42541 ttatcttgtt agaattatta caaccatggg tagaagtatg tccgaaaaac ctttaacgaa
    42601 aaccgattat ttaatgcgtt tacgtcgttg ccagacaatt gacacgctgg agcgtgttat
    42661 cgagaaaaat aaatacgaat tatcagataa tgaactggcg gtattttact cagccgcaga
    42721 tcaccgcctc gccgaattga ccatgaataa actatacgac aagatccctt cctcagtatg
    42781 gaaatttatt cgctaataaa taattcgctt tcggagctat aaccggctgt ttattaagaa
    42841 ttttatactt tttcaccatg aagacatacc ctatgtgatc tttatcacac agatgtaatg
    42901 ggaacgttct cttcactgac ttttcgtctt actgtgttgc cgcattttca gcaaccggag
    42961 gtcagtaatg agcacagaaa aagaaaagat gattgctggt gagttgtatc gctcggcaga
    43021 tgagacgtta tctcgcgatc gcctgcgcgc tcgtcagctt attcaccgat acaatcattc
    43081 cctggcggaa gagcacacat tacgccagca aattctcgct gatctattcg gtcaggtgac
    43141 agaggcttat attgagccaa cgtttcgctg tgactatggc tataacattt ttctcggtaa
    43201 taattttttc gccaacttcg attgcgtgat gcttgatgtc tgccctattc gcatcggtga
    43261 taactgtatg ttggcaccag gcgttcatat ctacacggca acacatccca tcgaccctgt
    43321 agcacgtaat agcggtgctg aactggggaa acccgtcacc atcggtaata acgtctggat
    43381 tggcggacgc gcggtcatta accccggtgt gaccattggt gataacgtcg tggtagcctc
    43441 aggtgcagtt gtcacaaaag atgtcccgga caacgttgtc gtgggcggta atccagccag
    43501 aataattaaa aaattgtaat cggtttttcg caactgttat gcaaaattgt ggtaaatctg
    43561 ttacttcccc tctactattc ccacgttaaa atagggtgtt ccctggaaag ttgcagatac
    43621 cacgaaggca aacgatgacc gaaatacaac gcctgctgac cgaaacgatt gagtctctga
    43681 atacccgcga aaaacgcgac aacaaacccc gctttagtat cagttttatc cgtaaacatc
    43741 cggggctgtt tatcggtatg tacgttgctt tttttgccac cctggcggtg atgttgcagt
    43801 ccgaaacgct gtcaggctct gtctggctac tggttgtatt atttatcctg cttaatggtt
    43861 tcttcttttt cgatgtctac ccacgctacc gctatgaaga tatcgacgtg ctggatttcc
    43921 gcgtttgcta taacggcgaa tggtacaaca cgcgctttgt acctgccgcg ctggttgaag
    43981 ccatcttgaa ctctccgcgt gtcgcggatg ttcataagga acaactgcaa aaaatgatcg
    44041 tccgtaaagg tgaactgtct ttttacgata tttttaccct cgctcgcgcc gaatcaacat
    44101 cttaagttag ggttacatac caggcgtaaa gctctgcgcc tggtcaaatg acaatgatcg
    44161 tttccaccca tcacttcatg aaataccagc tctacctcct tatctcctgc cagccttttt
    44221 ccacaatcag atatactttc cctacactgt gttaataagg atatgctggt gagaacacga
    44281 catctggtcg gccttatttc gggagtactg attctttcag tattgctgcc tgtcggctta
    44341 agcatctggc tggcccatca gcaggtagaa acatcgttta ttgaagagct ggatacctat
    44401 tcctcccgcg tcgctattcg ggccaataag gtggcgacac aagggaaaga tgcgctgcaa
    44461 gagctggaaa gatggcaagg cgctgcctgt agcgaagccc atctcatgga aatgcgtcgg
    44521 gtatcttaca gttatcgcta tattcaggaa gtggtttata tcgataacaa cgttccccag
    44581 tgttcgtctc tggagcatga aagtccgccc gataccttcc ccgagccagg taaaatttcg
    44641 aaagatggtt atcgtgtctg gttaacatcg cataacgatt taggcattat ccgttacatg
    44701 gtcgccatgg gaacggcaca ttatgtcgtc atgatcgacc ccgcttcctt tattgatgtc
    44761 attccctata gctcatggca aattgatgcc gccattattg gcaatgccca taacgttgtc
    44821 ataaccagca gcgatgaaat tgctcaggga attattacca ggctacaaaa aacacccggt
    44881 gagcatatcg aaaataatgg aatcatttac gatatcctgc ccttcccgga gatgaatatt
    44941 tcgatcatca cctgggcttc aacgaaaatg ttgcagaaag gctggcatcg gcaagtcttt
    45001 atttggttac cgctcgggtt ggtgattggc ctgctggcag cgatgtttgt gctgcgtatt
    45061 ttgcgccgta tccagtcacc gcatcatcgg ctgcaggatg ctatcgaaaa tcgtgatatt
    45121 tgcgtgcact atcagccgat tgtctcctta gccaatggca aaattgtcgg tgctgaggca
    45181 ctggcgcgct ggccgcagac agacggtagt tggttgtcac cagatagttt tattccgctg
    45241 gcacagcaaa cgggcctttc tgagccattg acgctactga ttataagaag cgtctttgaa
    45301 gatatgggcg actggctgcg tcagcatcca cagcagcata tttcgatcaa tcttgaatcc
    45361 accgtgctca cctcggaaaa aatcccgcaa ttgctgcgtg aaatgatcaa tcactatcag
    45421 gttaatccca gacagatcgc gcttgaactc actgaacgcg agtttgccga tccgaaaacc
    45481 agcgccccga taatttctcg ctaccgggag gcgggccatg aaatttatct tgatgatttt
    45541 ggtacgggat attcaagttt aagttattta caggatctgg atgtcgacat tctgaagatc
    45601 gataaatctt tcgttgatgc gctggaatat aaaaatgtca cgccgcatat catcgaaatg
    45661 gcaaaaacac tgaaactgaa aatggtagcg gagggaatcg aaaccagtaa acaagaagag
    45721 tggctacgcc agcatggcgt gcactacggt cagggctggc tctacagcaa ggcattaccg
    45781 aaagaagatt tcttacgctg ggccgagcaa catttgtgaa tttttgggct gacggcacgc
    45841 cgcacagccc gtcacgctac ctattcgtcg atgattgact cgaatccgcc atagatcatt
    45901 cgcttgccgt caaacggcat ggactcgccg aactctttca tccgtgggtc cgacatcatc
    45961 ttttgagtag cagcgtcgcg gacctctttt gaagggtatt caatccagct aaagacaacc
    46021 tcttcattct cttccgcttt caccgccata cgaaaatcgg tcactttgcc atccggtaca
    46081 tcgctggccc agcattcgac aatacgaagc gcgccaaact ctttaaacaa tggcgcagcc
    46141 ttagcggcca tttcccgata ggcatccttt ttatcggcag gaacggcaac cacaaaacca
    46201 tcaacatact tcatcagaca tacctccgaa gcgtactgcg cagccagaca attcttgcga
    46261 cgtcagttgt ggttaaagtt tagccatgtt ggggtttttt gacgaaacgg tgggaggcag
    46321 aaatgggtgg gggccctgcc agctacatcc cggcacacgc gtcatctgcc ttggctgctt
    46381 ccttccggac ctgacctggt aaacagagta gcgttgcggg agaaccaaca gagcccccat
    46441 tgagagcgtt gagaaccaac gcgcaggcgc attatcactg ctgccccggc taattgcaac
    46501 ccatcgcgtg ccagatgctg gtttcttgcg caatgcgact tcactcgggc gtttttccag
    46561 attatgctga aaaattacct caggaagaaa agatggaaaa agaagattca tttccccaac
    46621 gcgtctggca aatcgtcgcc gctattcccg aaggctatgt caccacttac ggtgatgtgg
    46681 cgaaactggc gggatcgccc cgcgccgcgc gccaggtggg cggtgtgtta aagcgtctcc
    46741 ctgaaggcag caccttaccc tggcaccggg tggttaatcg ccacggcaca atttcgctaa
    46801 ccggaccgga tttacagcgt cagcgacagg cattactggc agaaggtgtg atggtatcgg
    46861 gaagcgggca aatcgacttg cagcgttatc gctggaacta ctaaccctct ccgggcggag
    46921 agggttaacg tcgcttaata ttgcgtagga gctggaactg ccgaagacgg attcacctga
    46981 gttggtgaag tcgaaggtac ggtagtcgtt gcaccaccgc tggcctgaac cggcacggcg
    47041 gtttgctgca ccggcaccaa tgtcaggtcg gctttagttc cgcccttgtt gatcaccggc
    47101 tgaacggtat cggtaataaa taccagtttg tcattcacgg taatcgccgc actcaacaga
    47161 atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct gaatggtgac
    47221 tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc atcggctaac
    47281 gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac tttctgacgg
    47341 atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt tgctgaaata
    47401 gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc gcaagccgcc
    47461 aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca tttctcctta
    47521 ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca aaatagtaac
    47581 gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta attcagacca
    47641 ttgcacccat cggaccagta gcaaatttgc gttatactca actcactttg gcttgctgcg
    47701 gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact gacattgtta
    47761 aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt aggtttacgc
    47821 caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga gaccgtccct
    47881 gaagagcggc tggtacattc gtttcacagc tactttcttc gccctggcga tagtaagaag
    47941 ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc ccgccgggtt
    48001 gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca ggcaccagaa
    48061 gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg cctgccttcg
    48121 gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa agataaattc
    48181 atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact gaaaggtcac
    48241 gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc ggatgacctg
    48301 cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc ggtagctcta
    48361 cagccgcacg gcatcggttt tctcgaaccg gggattcaga ttgccaccat tgaccattcc
    48421 atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt ggagagcacc
    48481 tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccagga cggcgtgctg
    48541 gttgcctcga ccgttcagga aggggtgatg cgtaatcaca attaaaaaaa tagccggagg
    48601 taaaaccgtc cggctatttt ttgcagtgct tgttacgcgt tataggcatt ctcgccgtga
    48661 ctgttgacat ccagcccttc tcgctcctgc tcttccggta cacgcagacc aaccgtcaga
    48721 tccgccagtt tgtagccaat aaatgccacc acaccggacc agacgatcgt aatggcgatg
    48781 ctttccagct gtaccagcaa ctgatggccc atcgtcacac cttcagcgaa gcccacgcca
    48841 cccagcgaac tggcggcaaa aatcccagtc atgatacagc cgacaatgcc acaaacgccg
    48901 tgcacaccga agacatcgca gggatcatcc acccgcagca agcgtttgag catagtaacg
    48961 ccccacaagc ccgccagacc agctaccacg ccgataatca acgcgccgcc aaccccaatg
    49021 tagccacacg ctggcgtcac gccgaccaga ccggcaatcg cgccagaaca cgctcccagc
    49081 agagaaggct taccacgcag cgcccattca ccgaagatcc agccaagaat tgccgccgcc
    49141 gttgcgacca cagtattcac aaatgccagt gccgcgattt cattcgccgt gcccgctgac
    49201 ccggcgttaa agccaaacca accaatatag agaatggcag taccggtgaa gaccattggc
    49261 aggttgtgcg gtttaaacgc ctctttgccg aagcccacgc gtttgcctat cagatacgcg
    49321 cccaccagac cggcgattgc ggcgttaata tgcaccaccg tgccacccgc gaaatccagc
    49381 gcaccgtgag aagccagcaa accaccgccc cacaccatat gcgcaatcgg aatgtaagag
    49441 agcgtcagcc ataccaccac gaaaatcaac acagctgaga agcggattcg ttccgccagc
    49501 gccccaacga tcaagccgac ggtaatgcag gcaaacgagc cctgaaacgc cacgtggata
    49561 tactgataaa tgctgcccat caccgccgtc agttcgatgt tttttagcat caaccagtta
    49621 atgttgccga agaagttgtt gccctcacca aacgccagcg agtaaccgta aaccacccag
    49681 agaatacaga ccagtgcaaa cgtcaccgtc acctgcgtca gcatcgacag cacgtttttg
    49741 ccgcgaatca acccaccgta aaacagggca atccccggaa tagtcataaa cagcaccagc
    49801 gcagtacaaa tcatcataaa cgcattgtcg gctttatcgg ccaccgcagg tgcagccatt
    49861 accagtcccg gcagcatcgc cagtgaagca agcccagttt ttatcgtcgc tatcttcatt
    49921 tttcgttcct gttgctgtgt gccagagatt acagcgccgc ttcgtcggct tcgccggtac
    49981 gaatacgaat gacgcgttgc aattcagcga cgaagatttt gccgtcgcca atttttccgg
    50041 tgtaagccgc cttactgacg atatcgatca cttcatcgag ttggtcatca gcaatcgcca
    50101 catcaatttt tacttttggc aggaagttga cgctgtattc cgccccccgg tacagctcgg
    50161 catgcccttt ctgacgcccg aaacctttca cttcggtgac ggtcaggccc tgaataccaa
    50221 tggaagataa cgcttcacga acgtcttcca gcttgaatgg tttgattatc acggtcacca
    50281 gcttcataga tcccctccgg tcagaattcg gtaatggttc tgctacacga agaaggtatt
    50341 gcaagcggtg tgccagaaat gaaaaacgag aaagagagca ggaagttaag aagattgaaa
    50401 atgaaaacgc actatgacag tgcacagtgc gttacatttt tgcaccaacg atgaaatatt
    50461 gcgctattca ggcgctcaat gattcttctt cacgcacgct ggctgccagc tcttcgcccg
    50521 caagttgcag ttgatacatc tgccagtagc gtccctgggc cgccagcagt tgctggtgag
    50581 tgccctgctc cacggcttgc ccacgatgaa gcaccagaat ggtgtcggca tcaacaatgg
    50641 tcgataagcg gtgagcaatc accaccagcg tggtatgttc acgcacagcc gccagagcat
    50701 gttgaatcgc ctgttcagta ccggagtcaa tgctggcggt tgcctcatca aggatcagga
    50761 tttgcggcgt ctccaccaac acgcgtgcca gtgccagcag ttgcttttgc ccgacggaga
    50821 gattattccc ctgctcgccc agcggcgtat aaataccatc actcatgctg cgcgccagct
    50881 ccgccagttg cacggtttcc agcgcctgcc agacgcgttc ttcggagata tcacgcccca
    50941 gcgtcacgtt ggcgaggaag gtatccgcca gcaccaccgg atcttgctgc accattgcca
    51001 cgccctggtg cagcgcgctg tgacttagcg aacttaatgg acgaccatca aggcgaatct
    51061 caccttccgt tagcgggtaa tagcccatca ataaactggc gagggtgctt ttgccactgc
    51121 cggtatgccc gaccagtgcc acaaagttac gcgaaggcac agagagatta atgttcttta
    51181 gcaccagatt gtcatcgcga taagcaaatg acacgttatc gacttcgatg gtgccactct
    51241 gtaacgggcg atcatcattg ccatattgct ggcgcggtcc gtccatcagt tcaaacacgc
    51301 gctcaccagc aacaacagcc tgttgcagca tcgcctgttg cgtggtcagt tcgattaatg
    51361 gttcgttaag tcgcccaaga tagctgataa acgcatacag cacgcccact tcaatggtgc
    51421 cgctagcgga gaagccaaac agcatcaaca agccacaaag aatgagcgat gaaaacagac
    51481 tcagcagcgg acgcagcaga aaaccgtcga ggcgcagggt ttgcatcctg gccatatagt
    51541 gtgaacgact ggcctccccc atacgttcgc caaatcgcgc ctgctgacgg aactgctgga
    51601 taacgctcat gccattgatg atttcgttaa agccgtcgtt gatatccgct agataggcgc
    51661 gcacacgacg gacaatcggc gtgctgtaac gctggtatat caccattacc accagcacca
    51721 ccgggaaaat cattatcgcc accagcgcca ttcgccagtc gaggctgaac atcgccacca
    51781 gcatcgcgcc caccagtgcg gcgctacgca ggacagttgc cactacggta acgtagagat
    51841 cgcggatcac ttcagtgtca ttagtgacgc gggaaatcac ctgcccgacg ggttgggtat
    51901 caaactcgct taatggctgg cgcaatgcgg catccatcac gtcggtacgc aactgttgca
    51961 ctacgcctac tgccgcccga ttaaacagca gcgactgcgc gtaatgtagc ccggcggcaa
    52021 acagttgtag cccaacatac gccgcagcca gccctgcaac cattttcaac ggcaggttat
    52081 ttttcgctac catattgtcg ataaaatagc tgataagcag cggcccactg acttctgccg
    52141 ctgccgcaac ccacatcatc aggaccgcaa tccccagcgg tttacgccac ggcgaaccgt
    52201 acgctaacag acgcttgaga gtcggccaca gttggctaaa actacgcatc gacggcctcc
    52261 tcgcgaattt ccggagcgtc gtcgagcgcc gcctccagtt gttgatagcg atacatatcg
    52321 cgataccagc cgctttgttg cgccagctca tcatgattgc cacgctgggc gatatgtccg
    52381 tgctgcatca caataatttc actggcttcc gtcagtgccg aaaggcgatg ggcactgatg
    52441 attaccgttc ttccctgtcc ccactgacgc aggttatgca ggatctggtg ctctgtgcgt
    52501 ccgtccaccg ccgaaagcgc atcatcaagg atgaggattt ccgcgttgac taataacgct
    52561 cgagcaatgg agatacgctg tttttgccca ccggaaagca tcacgccgcg ctcgcccact
    52621 tctgtatcgt aaccttgcgg tagacgcaaa atatcgtcat gtacgctggc taaccgcgcg
    52681 acatgctcaa tctcttgctg ggtggcattc gggcaaccca acgcgatgtt gttcgctacg
    52741 gtgtcggaaa aaaggaacgg tgtctggcta actaccgcca ggcggctacg ccagctatcg
    52801 agttgtaact tcgtcagagg aatatcatga aagcgaatat ccccctcgct gacgtcgaaa
    52861 tgacgctgaa tgagcgacaa cagggtactt ttgccggaac cagtcggccc gcagataccc
    52921 agcatctgac cgggtttcag ggcgaaattg acgttttcca gcgcaggatg gtcagtctgc
    52981 ggatacgtga actggtgaat atttacatca agttcgccac gcccttccgg cacgggttcg
    53041 ctaccatcgt tcaccaccgg cgcttccgcc agcatcgcac gaatacggct gtacgcagca
    53101 ctaccacgtt ccacaatgtt aaacatccat gccagcgcca gcattggcca aatcatcaga
    53161 cctaaataca tcataaaact ggtgagctgg cccagcgtta aactgccctg cgccaccatc
    53221 caactaccgc cgccaatcgc cagcaagttc gccataccaa tcgcgatata gatggttggg
    53281 tcgaaacgag catcaatacg cgccacccgc atgttttttt tgccggtatc ttcggcatcc
    53341 gcggcaaata acgccgactg gcgatcttcc agaccaaagg ctttgatcat gcggatactg
    53401 gtgaggcttt cctgggtgcg gtcattaaga ctagaaaacg ccgcctgtgc cagcttaaag
    53461 cgttcatgca aggcatcgcc gttgcgcttg atcatgatcg ccatcactgg catcggcaac
    53521 agggcaaata aggtcaactg ccagctaatt tgcgtagaca tcataatcaa cacagcgcag
    53581 cccatcacca gtgaatccac cagcgtcagc accccttctc cggcggcaaa cacgacgcga
    53641 tcgacgtcat tggtcgcacg agccatgaga tcacccgtgc gatgacgcag gtaaaactca
    53701 ggatgctgcc ggcttagctg acggtaataa tcttcacgca gttcaacagc cagttgataa
    53761 gacgcaccaa acagcaatac ccgccagacg taacgcagga gataaaccac aacggcaatc
    53821 agcaccatgg tggcgatcca catcaggatc tgcccggtag taaagtgttg ttctgtcacg
    53881 ccatcgacaa caataccaac cacttttggc ggaaccagtt gcagcatcgc gataatgaca
    53941 agcaaggcga cagccccgag atagcgacgc cattcccgac ggaaatacca gcttaattga
    54001 gcaaataatc gcacgcgttg tgtcctgacc tgattctgga tatttattcg atgggtaaag
    54061 aagtggtgta tttaatctgt tccatcgcga agctggaagt gacgtccgac agccccggca
    54121 cgctgtttac cagacgctta taaaactcgt cgtagcgttt catgtcggca acctggacgc
    54181 gcatcagata atcgtattca ccagccatgc gccagaaccc cagcacttct ggcatttcgg
    54241 taaccaccgt gacaaagcgg caataccatt cgctgctgtg atgttgcgtt tttatcagca
    54301 caaaggcggt caggccgagg cctatttttt ccggatccag cagggcgact ttgccgataa
    54361 ggataccgtc gtcctccagc cgtttcaggc gcttccagca aggggtggtt gtcagattaa
    54421 cggcttcagc cagtgcctgc aaagagaggg tgcaatcctg ctgcagtaag gccagcagct
    54481 tacggtcaat tttatctaac atagcccttc cacggagaat ttttttctct tttaattcta
    54541 ttttaaaggt gaaatcgcga caatttattc tgtgcggttt aaacaccacg gcacaaaatg
    54601 acaaattcaa cgcaaaaaaa aaccgggcaa ttgcccggtg ctggaaggga tctcgttatt
    54661 cgggggaata aggtagatgt ggatagtcca gccagtgcgt caaatagtga gagacagcct
    54721 gatttcggca atgtccaatc accggtaaat gcgggagctc cgcgcgcagt tgcggcatcg
    54781 cattgcccat aataaacccg ctaccgacgc tgcctaacat ttcgcgatcg ttcatcgcat
    54841 caccaaaggc catgcaatcg cgcaacgata aacctaaatg ttgggtcagc accgtcaatg
    54901 cagcgccttt attgcagccc accggcagca cttcgaggca atccgtggcg gaaaaacaca
    54961 aatgtgcacg ctcgcctaat gcttcgtata gctggatctg caagcgtgta agatcgtcgt
    55021 gatcgccaca gaagcagatc ttggtgacgc tgccgagtgg catttttttg acatcgatta
    55081 tctgataacg aaaaccgcta tagacaaatg cctgcaacaa cgcagggatc tctttcccgg
    55141 taaaccaacc gtcgtcattg aagatatgca tgctggctcg ggtatcccat tgctgataca
    55201 gcaccagctc cgcgacatcc gcaggtaaat catcacgatg taaaagttca ccttccagag
    55261 aatgcacgcg cgttccgttg ccggtaatca aatatgcatc cagcgatagc gccccgagaa
    55321 tatgctgcat ctccagcgca tgacgccccg tggcaaaagt gagggtaatg tcgcgttcac
    55381 gcagtcgcgc caaagtagag agggttttct cacctaaatg atggtcaggc atcaataaag
    55441 tgccatccat atcaaatgct gccagacgag ccatttcttt ctccactctg tgacacggtt
    55501 aatggtgatt gagtatcacc tgatatatac ggaagtaata gtgaatagtt aaataatatt
    55561 gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc tgtggcaacc
    55621 ttcagccgga aagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
    55681 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
    55741 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
    55801 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
    55861 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccgtttatgg gcggacaatg
    55921 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
    55981 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
    56041 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
    56101 tactgacggg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
    56161 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
    56221 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
    56281 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
    56341 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
    56401 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
    56461 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
    56521 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aggtcagtag
    56581 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
    56641 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
    56701 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
    56761 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
    56821 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
    56881 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    56941 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    57001 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    57061 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    57121 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    57181 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    57241 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    57301 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    57361 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    57421 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    57481 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    57541 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    57601 ccgatggcat cccgaatacg tttgtccctg ggcgtaatat tttgttcctg acgctggcgg
    57661 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    57721 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    57781 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    57841 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    57901 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    57961 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    58021 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    58081 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    58141 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    58201 tccgactcca gtgtaatgac ctggcttaag atgccgcttt taccgtttaa ttgctgcaac
    58261 tgactggtaa tagttaacag gtcacttaat accgctgggc gacgatagtt aatattgata
    58321 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    58381 aacccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    58441 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    58501 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    58561 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    58621 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    58681 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    58741 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    58801 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgt tgtcggtttc
    58861 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagggta
    58921 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    58981 tcaccttaac ggcagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    59041 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    59101 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    59161 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    59221 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    59281 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    59341 gccgcctggc taatcgggtc acgaccggaa cggcttaaga ttttcggctc gccaaatttc
    59401 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    59461 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    59521 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgat gcgcagcacg
    59581 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccggcgc gccgttttca
    59641 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    59701 aggttatctt tgctaaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    59761 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    59821 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    59881 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    59941 gatttgatga caccagacag ttgccctttt tctttcagac cagcattttt cagttcgtcg
    60001 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    60061 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt cattcagctc atcaagtacc
    60121 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    60181 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    60241 tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    60301 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    60361 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    60421 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacacc gttaatcagc
    60481 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    60541 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    60601 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    60661 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    60721 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatgcga
    60781 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    60841 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    60901 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    60961 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    61021 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacgc
    61081 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    61141 acatccccac cggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    61201 agctcggtac tttagcagca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    61261 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    61321 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    61381 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    61441 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    61501 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgccatc
    61561 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    61621 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatacc agacggttca
    61681 ttttgtagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    61741 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    61801 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    61861 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    61921 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    61981 gtcgcacctt ccggaacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    62041 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    62101 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    62161 atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg accgacacgg
    62221 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    62281 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    62341 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    62401 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    62461 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    62521 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    62581 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    62641 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    62701 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt tttcacgccc
    62761 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    62821 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    62881 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    62941 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    63001 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    63061 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    63121 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    63181 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    63241 gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    63301 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    63361 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    63421 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    63481 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    63541 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    63601 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    63661 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    63721 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    63781 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    63841 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    63901 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    63961 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    64021 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    64081 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    64141 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    64201 ctgttgcgct tccggcttgc cataaatcag caacggtttg ctttgaccat caattaccga
    64261 ctcgtcgata accacttttt cgacatcttc catggacggc agatcgtaca tggtatcgag
    64321 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    64381 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    64441 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    64501 ttcttcgctc agttcattca acgttgcgac aaccggcaga cgaccaatga actcagggat
    64561 cagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    64621 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    64681 atgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    64741 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    64801 agctaccgta ccttcgatca gtttcaacag tgcctgctgt acgccttcac cggaaacgtc
    64861 acgggtaata gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    64921 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    64981 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    65041 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    65101 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    65161 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    65221 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    65281 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    65341 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    65401 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    65461 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    65521 gcgcaagccg cttacagcgt tgtgccgccc tggataagta tagcggcaca gttgcgcctc
    65581 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    65641 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    65701 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    65761 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    65821 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca gcaagaaagc gcccatcgag
    65881 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    65941 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    66001 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    66061 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    66121 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    66181 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaacct
    66241 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
    66301 catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt
    66361 tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
    66421 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
    66481 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
    66541 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
    66601 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
    66661 cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
    66721 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
    66781 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
    66841 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
    66901 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
    66961 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
    67021 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt
    67081 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
    67141 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
    67201 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
    67261 ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac
    67321 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
    67381 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
    67441 aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
    67501 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
    67561 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
    67621 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
    67681 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
    67741 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
    67801 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
    67861 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
    67921 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
    67981 gtctgaatgc tgggcaaaaa gcgcaaaatt cagacaaaac aaaacggccc gtaggccgtt
    68041 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
    68101 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
    68161 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
    68221 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
    68281 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
    68341 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
    68401 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
    68461 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
    68521 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
    68581 agtgtttctt ttgccagggg cgattatttc gtcaacatga tgaatttaca tgccttaccc
    68641 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
    68701 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
    68761 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
    68821 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    68881 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc
    68941 gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    69001 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    69061 tcccagggca acgtgcgcta taacatcgct accaaagcgg atatcgccat catcgctacc
    69121 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    69181 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    69241 gctgatatgt ctcaggacac cagcattcac gaatttatca agcagaacgc gcgttaattt
    69301 ctgccctctg gcccggtgca aaccgggcct gtagacgccc atgtccagtc aatatttacg
    69361 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    69421 gctcgccctg acatccggta ccttacaggc ctggatgacg gtcgagaata ttgatctcaa
    69481 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    69541 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    69601 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaacctg gcacccaact
    69661 ccgctggatg gcggcactgg cggtggtgat cgctttttgc tcggcctccc aggatattgt
    69721 cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat
    69781 cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcttagccc tgtggctggc
    69841 agataaatgg ctgggctggc agggcatgta ctggttgatg gcggcgctgt tgatcccttg
    69901 tattatcgcg acgttgcttg caccagaacc gaccgacacc attcctgtgc caaaaacgct
    69961 ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg cctggcttat
    70021 tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc tgacaaccac
    70081 gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtta acaaaacgct
    70141 tggcttactg gcgaccattg tgggcgcatt gtacggtggg attttgatgc agcgcctgtc
    70201 gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg ctggttactg
    70261 gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct ttttcgaaaa
    70321 cctctgtggc gggatgggca catcagcctt tgtcgcgctg ttaatgacgc tgtgtaataa
    70381 gtcattttcc gcaactcaat ttgccctgct ctcagcgctt tccgctgtag gacgagttta
    70441 tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat tctatctatt
    70501 ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc agacgcttga
    70561 atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccggcag gttatgcctt
    70621 tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac tgctgttgac
    70681 gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg aagtcggggt
    70741 tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg cgctacgaaa
    70801 aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg ccgtaattat
    70861 tacggctaaa taaacatcag tatcgcttaa taatat
//
LOCUS       DAZFYC010000009        70177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFYC010000009 DAZFYC010000000
VERSION     DAZFYC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.009"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(278..754)
                     /gene="ybhB"
                     /locus_tag="ACHKW5_000745"
     CDS             complement(278..754)
                     /gene="ybhB"
                     /locus_tag="ACHKW5_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO7510184.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPARTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(813..2102)
                     /gene="bioA"
                     /locus_tag="ACHKW5_000746"
     CDS             complement(813..2102)
                     /gene="bioA"
                     /locus_tag="ACHKW5_000746"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO7510185.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRH
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2189..3229
                     /gene="bioB"
                     /locus_tag="ACHKW5_000747"
     CDS             2189..3229
                     /gene="bioB"
                     /locus_tag="ACHKW5_000747"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO7510186.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3226..4380
                     /gene="bioF"
                     /locus_tag="ACHKW5_000748"
     CDS             3226..4380
                     /gene="bioF"
                     /locus_tag="ACHKW5_000748"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO7510187.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            4367..5122
                     /gene="bioC"
                     /locus_tag="ACHKW5_000749"
     CDS             4367..5122
                     /gene="bioC"
                     /locus_tag="ACHKW5_000749"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO7510188.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            5115..5792
                     /gene="bioD"
                     /locus_tag="ACHKW5_000750"
     CDS             5115..5792
                     /gene="bioD"
                     /locus_tag="ACHKW5_000750"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7510189.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6371..8392
                     /gene="uvrB"
                     /locus_tag="ACHKW5_000751"
     CDS             6371..8392
                     /gene="uvrB"
                     /locus_tag="ACHKW5_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO7510190.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8584..9492)
                     /gene="yvcK"
                     /locus_tag="ACHKW5_000752"
     CDS             complement(8584..9492)
                     /gene="yvcK"
                     /locus_tag="ACHKW5_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO7510191.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      9757..9901
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9889..10878
                     /gene="moaA"
                     /locus_tag="ACHKW5_000753"
     CDS             9889..10878
                     /gene="moaA"
                     /locus_tag="ACHKW5_000753"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO7510192.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            10900..11412
                     /gene="moaB"
                     /locus_tag="ACHKW5_000754"
     CDS             10900..11412
                     /gene="moaB"
                     /locus_tag="ACHKW5_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO7510193.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11415..11900
                     /gene="moaC"
                     /locus_tag="ACHKW5_000755"
     CDS             11415..11900
                     /gene="moaC"
                     /locus_tag="ACHKW5_000755"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO7510194.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            11893..12138
                     /gene="moaD"
                     /locus_tag="ACHKW5_000756"
     CDS             11893..12138
                     /gene="moaD"
                     /locus_tag="ACHKW5_000756"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO7510195.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12140..12592
                     /gene="moaE"
                     /locus_tag="ACHKW5_000757"
     CDS             12140..12592
                     /gene="moaE"
                     /locus_tag="ACHKW5_000757"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO7510196.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            12729..13433
                     /gene="ybhL"
                     /locus_tag="ACHKW5_000758"
     CDS             12729..13433
                     /gene="ybhL"
                     /locus_tag="ACHKW5_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7510197.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            13578..14282
                     /locus_tag="ACHKW5_000759"
     CDS             13578..14282
                     /locus_tag="ACHKW5_000759"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO7510198.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVLEFINGLVW"
     gene            14352..15065
                     /locus_tag="ACHKW5_000760"
     CDS             14352..15065
                     /locus_tag="ACHKW5_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7510199.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15101..16057)
                     /gene="ybhN"
                     /locus_tag="ACHKW5_000761"
     CDS             complement(15101..16057)
                     /gene="ybhN"
                     /locus_tag="ACHKW5_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO7510200.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16057..17298)
                     /gene="clsB"
                     /locus_tag="ACHKW5_000762"
     CDS             complement(16057..17298)
                     /gene="clsB"
                     /locus_tag="ACHKW5_000762"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO7510201.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17295..18056)
                     /gene="ybhP"
                     /locus_tag="ACHKW5_000763"
     CDS             complement(17295..18056)
                     /gene="ybhP"
                     /locus_tag="ACHKW5_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7510202.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18189..18599
                     /gene="ybhQ"
                     /locus_tag="ACHKW5_000764"
     CDS             18189..18599
                     /gene="ybhQ"
                     /locus_tag="ACHKW5_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO7510203.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18561..19667)
                     /gene="ybhR"
                     /locus_tag="ACHKW5_000765"
     CDS             complement(18561..19667)
                     /gene="ybhR"
                     /locus_tag="ACHKW5_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7510204.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19678..20811)
                     /gene="ybhS"
                     /locus_tag="ACHKW5_000766"
     CDS             complement(19678..20811)
                     /gene="ybhS"
                     /locus_tag="ACHKW5_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7510205.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20804..22540)
                     /gene="ybhF"
                     /locus_tag="ACHKW5_000767"
     CDS             complement(20804..22540)
                     /gene="ybhF"
                     /locus_tag="ACHKW5_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7510206.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22533..23528)
                     /gene="hlyD"
                     /locus_tag="ACHKW5_000768"
     CDS             complement(22533..23528)
                     /gene="hlyD"
                     /locus_tag="ACHKW5_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO7510207.1"
                     /translation="MKKPVVIRLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23531..24202)
                     /gene="cecR"
                     /locus_tag="ACHKW5_000769"
     CDS             complement(23531..24202)
                     /gene="cecR"
                     /locus_tag="ACHKW5_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO7510208.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24431..25795
                     /gene="rhlE"
                     /locus_tag="ACHKW5_000770"
     CDS             24431..25795
                     /gene="rhlE"
                     /locus_tag="ACHKW5_000770"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO7510209.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(26027..26509)
                     /gene="ybiA"
                     /locus_tag="ACHKW5_000771"
     CDS             complement(26027..26509)
                     /gene="ybiA"
                     /locus_tag="ACHKW5_000771"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO7510210.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26629..28779
                     /gene="dinG"
                     /locus_tag="ACHKW5_000772"
     CDS             26629..28779
                     /gene="dinG"
                     /locus_tag="ACHKW5_000772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO7510211.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28807..29769
                     /gene="ybiB"
                     /locus_tag="ACHKW5_000773"
     CDS             28807..29769
                     /gene="ybiB"
                     /locus_tag="ACHKW5_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO7510212.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            29910..30995
                     /gene="hcxB"
                     /locus_tag="ACHKW5_000774"
     CDS             29910..30995
                     /gene="hcxB"
                     /locus_tag="ACHKW5_000774"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO7510213.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31062..31121
                     /gene="ybiE"
                     /locus_tag="ACHKW5_000775"
     CDS             31062..31121
                     /gene="ybiE"
                     /locus_tag="ACHKW5_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO7510214.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31224..31484)
                     /gene="ybiJ"
                     /locus_tag="ACHKW5_000776"
     CDS             complement(31224..31484)
                     /gene="ybiJ"
                     /locus_tag="ACHKW5_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO7510215.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31749..32015)
                     /gene="ybiI"
                     /locus_tag="ACHKW5_000777"
     CDS             complement(31749..32015)
                     /gene="ybiI"
                     /locus_tag="ACHKW5_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO7510216.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32089..32766)
                     /gene="ybiX"
                     /locus_tag="ACHKW5_000778"
     CDS             complement(32089..32766)
                     /gene="ybiX"
                     /locus_tag="ACHKW5_000778"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO7510217.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32808..35090)
                     /gene="fiu"
                     /locus_tag="ACHKW5_000779"
     CDS             complement(32808..35090)
                     /gene="fiu"
                     /locus_tag="ACHKW5_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO7510218.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(35354..35614)
                     /gene="mcbA"
                     /locus_tag="ACHKW5_000780"
     CDS             complement(35354..35614)
                     /gene="mcbA"
                     /locus_tag="ACHKW5_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO7510219.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            35890..36816
                     /gene="rlmF"
                     /locus_tag="ACHKW5_000781"
     CDS             35890..36816
                     /gene="rlmF"
                     /locus_tag="ACHKW5_000781"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO7510220.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(36813..39038)
                     /gene="ybiO"
                     /locus_tag="ACHKW5_000782"
     CDS             complement(36813..39038)
                     /gene="ybiO"
                     /locus_tag="ACHKW5_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO7510221.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(39155..39877)
                     /gene="glnQ"
                     /locus_tag="ACHKW5_000783"
     CDS             complement(39155..39877)
                     /gene="glnQ"
                     /locus_tag="ACHKW5_000783"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO7510222.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(39874..40533)
                     /gene="glnP"
                     /locus_tag="ACHKW5_000784"
     CDS             complement(39874..40533)
                     /gene="glnP"
                     /locus_tag="ACHKW5_000784"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO7510223.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(40672..41418)
                     /gene="glnH"
                     /locus_tag="ACHKW5_000785"
     CDS             complement(40672..41418)
                     /gene="glnH"
                     /locus_tag="ACHKW5_000785"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO7510224.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(41822..42325)
                     /gene="dps"
                     /locus_tag="ACHKW5_000786"
                     /gene_synonym="pexB"
     CDS             complement(41822..42325)
                     /gene="dps"
                     /locus_tag="ACHKW5_000786"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO7510225.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(42624..43511)
                     /gene="rhtA"
                     /locus_tag="ACHKW5_000787"
     CDS             complement(42624..43511)
                     /gene="rhtA"
                     /locus_tag="ACHKW5_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO7510226.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            43746..43811
                     /locus_tag="ACHKW5_000788"
     CDS             43746..43811
                     /locus_tag="ACHKW5_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510227.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            43864..44379
                     /gene="ompX"
                     /locus_tag="ACHKW5_000789"
     CDS             43864..44379
                     /gene="ompX"
                     /locus_tag="ACHKW5_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO7510228.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(44428..46011)
                     /gene="opgE"
                     /locus_tag="ACHKW5_000790"
     CDS             complement(44428..46011)
                     /gene="opgE"
                     /locus_tag="ACHKW5_000790"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7510229.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(46283..46411)
                     /gene="mntS"
                     /locus_tag="ACHKW5_000791"
     CDS             complement(46283..46411)
                     /gene="mntS"
                     /locus_tag="ACHKW5_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO7510230.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            46597..47064
                     /gene="mntR"
                     /locus_tag="ACHKW5_000792"
     CDS             46597..47064
                     /gene="mntR"
                     /locus_tag="ACHKW5_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO7510231.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            47061..48179
                     /gene="ybiR"
                     /locus_tag="ACHKW5_000793"
     CDS             47061..48179
                     /gene="ybiR"
                     /locus_tag="ACHKW5_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO7510232.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLCAALVGYVLLVILPAN"
     gene            complement(48238..49158)
                     /gene="ldtB"
                     /locus_tag="ACHKW5_000794"
     CDS             complement(48238..49158)
                     /gene="ldtB"
                     /locus_tag="ACHKW5_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7510233.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            49377..50969
                     /gene="ybiT"
                     /locus_tag="ACHKW5_000795"
     CDS             49377..50969
                     /gene="ybiT"
                     /locus_tag="ACHKW5_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO7510234.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(51206..52471)
                     /gene="ybiU"
                     /locus_tag="ACHKW5_000796"
     CDS             complement(51206..52471)
                     /gene="ybiU"
                     /locus_tag="ACHKW5_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO7510235.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(52623..53438)
                     /gene="ybiV"
                     /locus_tag="ACHKW5_000797"
     CDS             complement(52623..53438)
                     /gene="ybiV"
                     /locus_tag="ACHKW5_000797"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO7510236.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(53584..56016)
                     /gene="ybiW"
                     /locus_tag="ACHKW5_000798"
     CDS             complement(53584..56016)
                     /gene="ybiW"
                     /locus_tag="ACHKW5_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO7510237.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(56022..56921)
                     /gene="ybiY"
                     /locus_tag="ACHKW5_000799"
     CDS             complement(56022..56921)
                     /gene="ybiY"
                     /locus_tag="ACHKW5_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO7510238.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            57052..57714
                     /gene="fsa"
                     /locus_tag="ACHKW5_000800"
     CDS             57052..57714
                     /gene="fsa"
                     /locus_tag="ACHKW5_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7510239.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            complement(57790..58539)
                     /gene="moeB"
                     /locus_tag="ACHKW5_000801"
     CDS             complement(57790..58539)
                     /gene="moeB"
                     /locus_tag="ACHKW5_000801"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO7510240.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(58539..59774)
                     /gene="moeA"
                     /locus_tag="ACHKW5_000802"
     CDS             complement(58539..59774)
                     /gene="moeA"
                     /locus_tag="ACHKW5_000802"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO7510241.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASNV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            59978..60943
                     /gene="iaaA"
                     /locus_tag="ACHKW5_000803"
     CDS             59978..60943
                     /gene="iaaA"
                     /locus_tag="ACHKW5_000803"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7510242.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            60930..62801
                     /gene="gsiA"
                     /locus_tag="ACHKW5_000804"
     CDS             60930..62801
                     /gene="gsiA"
                     /locus_tag="ACHKW5_000804"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO7510243.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            62821..64359
                     /gene="gsiB"
                     /locus_tag="ACHKW5_000805"
     CDS             62821..64359
                     /gene="gsiB"
                     /locus_tag="ACHKW5_000805"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO7510244.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            64377..65297
                     /gene="gsiC"
                     /locus_tag="ACHKW5_000806"
     CDS             64377..65297
                     /gene="gsiC"
                     /locus_tag="ACHKW5_000806"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO7510245.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            65300..66211
                     /gene="gsiD"
                     /locus_tag="ACHKW5_000807"
     CDS             65300..66211
                     /gene="gsiD"
                     /locus_tag="ACHKW5_000807"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO7510246.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            66389..68737
                     /gene="pdeI"
                     /locus_tag="ACHKW5_000808"
     CDS             66389..68737
                     /gene="pdeI"
                     /locus_tag="ACHKW5_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7510247.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            68745..70073
                     /gene="dgcI"
                     /locus_tag="ACHKW5_000809"
     CDS             68745..70073
                     /gene="dgcI"
                     /locus_tag="ACHKW5_000809"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7510248.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT        16789 a        17608 c        18750 g        17030 t
ORIGIN      
        1 gaggacaaaa aatcaccttg cgctaatgct ctgtctcagg tcactaatac tatctaaata
       61 gttgattcat agtgactggg tatgttgtgt tttgtcgtat tttgtagtct attatttaga
      121 ctgagtcaat tataagacgt tgatattaat ggtttttact gtttcacatt caactttttt
      181 atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa
      241 gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc gcagtaatcg
      301 aggcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggcacctt
      361 catcgacatc aatacgttct acatccagcg cgtgaacggt aaaaatgtag cgatgagttt
      421 cgcctttcgg cggtgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca
      481 aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgtgtat
      541 cagcgggtaa gttaacaact acccagtgcc accagccgga gccggttggc gcatccgggt
      601 cgtagcaggt gacaacaaaa cttttcgttc tcgcaggaac ttcatcccac gccagatgcg
      661 gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat
      721 cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa
      781 gtgtagccag aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt
      841 accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc
      901 ggcatcaggt aaatcagttt gccaaaaggc cggatccaga caccctgttc gacaaagaat
      961 ttttgcagcg ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt
     1021 acgcgcacat cggcaaccat ttcggcatca cgggcggggg caagttgctc gcgcagctgt
     1081 acttcaatag ccgccacctg ctgctgccat tcgccggatt cgataatcgc caggctggcg
     1141 tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccgtgcat aaagcaaccg
     1201 gcttcgccgt tactgatggt ttccgcaacc tcgcgcgtgg tgagcgtggc ggaaagggtc
     1261 attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat
     1321 tcacaggcaa acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc
     1381 aagatacctt cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg
     1441 cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga
     1501 tgcgccgcca tcaggcgggc aaaaccaacc atatcgcgct catcccattc accatccatg
     1561 cggctttgcg gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc
     1621 attgagttgt ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg
     1681 cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt
     1741 ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagtggt
     1801 tgcggcgtca tcgccaccag tttgcggcac agctcaatgg ctggcgcatg agtgataccg
     1861 ccaaacatca catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg
     1921 tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gtgtctgccg
     1981 tcagacaaaa ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag
     2041 gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaatatc gtccgttgtc
     2101 ataatcgact tgtaaaccaa attgaaaaga tttaggttta cgagtctaca ccgaattaac
     2161 aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca
     2221 agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca
     2281 tcgacagcat ttcgatcctc gtcaggtgca ggtcagcact ttgctgtcga ttaagaccgg
     2341 agcttgtccg gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga
     2401 agccgagcgg ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagctgc
     2461 gggatcgacg cgtttctgta tgggcgcggc gtggaagaat ccccacgaac gcgatatgcc
     2521 gtacctggaa caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct
     2581 gggcacgttg agtgaatctc aggcgcagcg ccttgcgaat gccgggctgg attactacaa
     2641 ccacaacctg gacacctcgc cggagtttta cggcaatatc atcaccaccc gcacttatca
     2701 ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gttctggcgg
     2761 cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa
     2821 cctgccgacg ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc
     2881 gcttgccgat aacgatgatg tcgatgcctt tgattttatt cgcaccattg cggtcgcgcg
     2941 gatcatgatg ccaacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca
     3001 gactcaggcg atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct
     3061 gaccacgccg aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa
     3121 tccgcagcaa actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttgaacaggc
     3181 gctgatgacc ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag
     3241 aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatcca
     3301 gtggcgcaag gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc
     3361 agtaacgatt atttaggttt aagccatcat ccgcaaatta tccgtgcctg gcagcagggt
     3421 gcggagcaat ttggcgtcgg tagcggcggt tccggtcacg tcagcggtta tagcgttgcg
     3481 catcaggcgc tggaagaaga actggccgag tggctgggct attcgcgggc actgctattt
     3541 atctctggtt ttgccgctaa tcaggcagtt attgccgcga tgatggcgaa agaggaccgt
     3601 attgctgccg accggcttag ccatgcctca ttgctggaag ctgccagttt aagcccgtcg
     3661 cagcttcgcc gttttgctca taacgatgtc actcatttgg cgcgattgct tgcttccccc
     3721 tgtccggggc agcaaatggt ggtgacagaa ggcgtgttca gcatggacgg cgatagtgcg
     3781 ccactggcgg aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat
     3841 gcccacggca cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta
     3901 aaaccagaat tgctggtagt gacttttggc aaaggatttg gcgtcagcgg ggcagcggtg
     3961 ctttgctcca gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc
     4021 accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgcagt
     4081 gaagaggggg acgctcggcg cgaaaaactg gcggcactca ttacgcgttt tcgtgccgga
     4141 gtgcagggtt cgccgtttac gcttgcggat tcacgtagcg ccatccagcc attgatcgtc
     4201 ggtgataaca gccgtgcgtt acagctggca gaaaaactgc gccagcaagg ttgctgggtg
     4261 acggcgattc gcccgccaac cgtacccgct ggcaccgcgc ggctacgcct gaccttaacc
     4321 gccgcccatg aaatgcagga tattgaccgt ttgctggaga tgctgcatgg caacggttaa
     4381 taaacaagcc attgcagcgg catttggtcg ggcagccgcg cactatgagc aacacgcaga
     4441 tctacagcgc cagagtgctg acgtcttact ggcaatgctc ccacagcgta aatacaccca
     4501 cgtactggac gcggggtgtg ggccaggctg gatgagccgc cactggcgag agcgtcacgc
     4561 gcaggtgacg gcgttagatc tctcgccgcc aatgcttgtt caggcacgcc agaaggatgc
     4621 cgcagaccat tatctggcgg gagatatcga atccctgccg ttagcgactg cgacgttcga
     4681 tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga
     4741 gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc
     4801 gttacccgaa ctgcatcagg cgtggcaggc agtggacgag cgtccgcatg ctaatcgctt
     4861 tttaccacca ggtgcaatcg aacagtcact gaacggcgtg cattatcaac atcatattca
     4921 gcccatcacg ctgtggtttg atgatgcgct cagtgccatg cgttcgctga aaggcattgg
     4981 agccacgcat cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg
     5041 attgcaactg gcctggccac aaaaacaggg gcgatatccc ctgacgtatc atcttttttt
     5101 gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga
     5161 aaactgtcgc cagttgtgca cttttacaag ccgcaaaggc agtaggttat cggacggcag
     5221 gttataaacc ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg
     5281 cgctggcgtt acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca
     5341 ccttcgcaga acccacctcg ccgcacatca tcagcgcgca agagggcagg ccgatagaat
     5401 cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg
     5461 aaggtgctgg cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa
     5521 cacaggaaca actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg
     5581 cgatgttgac tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga
     5641 acgatgttac gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga
     5701 ttcccgcgcc gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa
     5761 ccggaaagta cataaacctt gccttgttgt agccatcctg tatttggtta aattgcgagc
     5821 gagatcgcgt cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa
     5881 aaattttact tccgcctcat gcggcgaatg tgggaattac ccaggcggcg ggggataggg
     5941 gctggagaca gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac
     6001 gaggcaagcg agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa
     6061 atatctttta ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc
     6121 ttgccataaa actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga
     6181 taacccggta aggccggagt tttatctcgc cacagagtaa attttgctca tgattgacag
     6241 cggagtttac gctgtcacag aaatattatg gtgatgaact gtttttttat ccagtataat
     6301 ttgttggcat aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag
     6361 gtagcgactc atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca
     6421 gccagaggcg attcgacgtc tcgaagaggg gctggaagat ggcctggcgc accagacgtt
     6481 acttggcgtg actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca
     6541 gcgcccaacc atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat
     6601 gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca
     6661 gccggaagcc tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga
     6721 acatattgag cagatgcgtt tatccgccac caaagcgatg ctggagcgac gtgatgtggt
     6781 tgtagtggcg tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat
     6841 gatgctccat ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc
     6901 ggagctgcaa tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg
     6961 cgaggtgatt gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt
     7021 tgacgaggaa gtggaacgat tgtcgttatt tgacccgcta accgggcaga ttgtttccac
     7081 cattccacgt tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt
     7141 acaggcgatg gaggagatca aagaagagct ggccgccaga cgcaaagtgc tattggaaaa
     7201 caacaaactg ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat
     7261 gaacgagctg ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg
     7321 accgggtgag ccaccgccga cgctgtttga ttacctccct gccgatgggc tgctggtggt
     7381 cgatgaatct catgtcacca ttcctcaaat tggcggcatg tatcgcggtg accgggcgcg
     7441 taaagagaca ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct
     7501 taagtttgaa gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg
     7561 taattacgag ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg
     7621 attgcttgac ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc
     7681 ggagattcgt cagcgagcgg caattaacga acgcgtactg gtcaccacac tgaccaagcg
     7741 gatggcggaa gatcttaccg aatatctcga agaacatggc gagcgcgtgc gttatcttca
     7801 ctcagatatc gacaccgtcg aacgtatgga gattatccgc gacttgcgtc tgggtgagtt
     7861 cgacgtgctg gtagggatca acttactgcg cgaaggtctg gatatgccgg aagtgtcgct
     7921 ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca
     7981 gaccattggt cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataagat
     8041 caccccatca atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa
     8101 gtacaacgag gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct
     8161 ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat
     8221 tgttgagccg gataatgtgc cgatggatat gtcgccgaaa gcgttgcagc agaaaatcca
     8281 tgaactggaa gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca
     8341 aattcgtgac cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag
     8401 cgaagaagac tgatgacaaa cggaaaacag cctgatgtgc tacgcttatc aggcctacat
     8461 tttctccgca atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca
     8521 tccggcataa acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt
     8581 ccgttaacct aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg
     8641 acgatacgga atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc
     8701 cgagacatcc acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg
     8761 ctccataatt gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg
     8821 ccccagattg ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc
     8881 cttcagcagc agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag
     8941 atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa
     9001 taactcttga atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc
     9061 ctgatcgtca atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc
     9121 cactttcagc agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc
     9181 cagcgccttt aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccaccaaa
     9241 acggtattca aacatcgcgg aggcgacgct cggttccgtt atcagctggt tgaggcagtt
     9301 gcgcatatcg ccccaggcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc
     9361 attatcggtg gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag
     9421 aacgcgtccc agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt
     9481 acgattgcgc atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg
     9541 gggaaacagc aattaacctg ccaatattga ggatacaaac tattttcttc tacctctaaa
     9601 ggacgatgca cgctatgcct ccctgatgat gtatatcaaa gtaaagccgc cattttccct
     9661 tattctgtag cgaaatagca cgatcatgac gctatataca tgattacata gcgaaagtgt
     9721 ggatggtaaa aatctcattt acacgctagt atcggcataa ccactaaaca ctctagcctc
     9781 tgcacctggg tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc
     9841 agggcgaagg aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa
     9901 ctgactgatg catttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt
     9961 aactttcgtt gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa
    10021 ggctttctta ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc
    10081 gaaaaagtgc gcctgacagg cggtgagccg tctttacgcc gcgactttac cgatatcatc
    10141 gccgctgtgc gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt
    10201 ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc
    10261 gacagtctgg acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc
    10321 atggcgggga ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg
    10381 ctgatgcgtg atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc
    10441 cctatccagc tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt
    10501 aagcatcaca tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac
    10561 caattacgtc aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga
    10621 gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc
    10681 gtttcctcca ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc
    10741 gatctgctgg aagacgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg
    10801 cgggagaaga aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta
    10861 tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa
    10921 tttatcccga cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat
    10981 acctccggtc actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgataaa
    11041 gccattgtga aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc
    11101 gacgatgtac aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct
    11161 cccgaagcat tgctgccgtt gttcgaccgt gaagttgaag gttttggtga agtgttccgg
    11221 atgttgtctt ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc
    11281 gccaacaaaa cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg
    11341 gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat
    11401 ttgaagaaat aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg
    11461 tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca
    11521 tgcgcagcga gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg
    11581 ccactgcgcg tattgccggt attcaggcgg caaaacgcac ctgggatctg atcccgctct
    11641 gtcatccgct gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc
    11701 gggtgcgtat agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcat
    11761 taaccgcggc ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag
    11821 atatggtgat tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta
    11881 aggtggaagc ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca
    11941 gatgcaaccg aagtggctgc ggatttccca actgttgaag cgttacgcca gcacatggct
    12001 gcgcagagcg atcgctgggc gctggcgctg gaagatggca aattactggc tgccgtcaac
    12061 cagacgctgg tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg
    12121 ccggtaaccg gaggttaaga tggcagaaac caaaattgtt gttggtccgc agccgttcag
    12181 cgtaggagaa gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt
    12241 tactggtaag gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca
    12301 ctatccgggg atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg
    12361 gccgctgggg cgcgtcactg tgattcaccg catcggggaa ttatggccgg gcgatgaaat
    12421 cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat
    12481 tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga
    12541 ccgctgggtt gaagcccggg agagcgatca gcaggcggca aaacgctggt agttttttgt
    12601 tagccggata aggcaccgct gcgtccggca atttcaccgc tcagtacata cgtttacgct
    12661 gcgtcgatgc acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg
    12721 agatcgtcat ggacagattc ccacgttctg attcaatcgt acaaccccgg gctggcttgc
    12781 aaacttatat ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg
    12841 ttgcctggta tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct
    12901 ttttaatcgg tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc
    12961 aaaagctgag cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc
    13021 ttacgctttc cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg
    13081 ttactgccgg gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa
    13141 gtggctttgg caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca
    13201 acttctggtt gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct
    13261 ttgtcggatt gacggcgtat gacacgcaga aactgaaaaa tatgggtgag cagattgata
    13321 cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg cgcgttaacc ttgtatctgg
    13381 acttcatcaa cctgttcctg atgttgttgc ggatcttcgg caaccgccgt taaattctct
    13441 tcttcctgct gatcccggcc tggagccggg atttttttcg gattttataa agactattcc
    13501 gctcatttac atgagaactg aatcgcggca aatgccagac tatgtccact acgtaatttc
    13561 agggacagat aacgcatttg aacagacaat ccactgctac tcaattacag caaaaaagtg
    13621 cttcgctcag caaaatgctg cttctcattt ggtggttaac agcagctgga gtatgggcta
    13681 cggcaggctg tgcctggata tttcagcatt tgcattgggt tacgtcacaa aagtctccag
    13741 gtgcgtttgt cttcgctttg gctgcgctga taataggtgg tctctggtta aacaacgccc
    13801 tgaaaagact gaatgtatca ggcgcatgtc tggtctatct gctaataatc cttcttgttg
    13861 gctttctctt cgccagcctt tatacctttt cgagcatagc gacggtaatg ggaattacgg
    13921 gagggatgtt tgccgcaatg gcgctgattt gctcttgtag taatagggtt atcccaccag
    13981 tcagacagtt atattcatat attttctgtg gtttgagtat tgcttttgtt gttaacctga
    14041 tccttaccag cagtttttcc gtgtggttag cgagtatttt gactgtcttt atctggggca
    14101 ttacggctgc ctgtgaagca acaacactgg aagatttgat ccgtgttgcc gacacctctg
    14161 aaatttcagg ttctcttcgc tgtatcgttc ctggggcaat aacactctat tttagtattt
    14221 taagcgtcct ctttagaatt actgtaacag ttcttgaatt tattaatggt ttagtgtggt
    14281 agtgcgaatt gacgagtttt attgcgttaa ttggaataga taatgatatt taacttaagg
    14341 atataaaaat aatgaattcg cgctcagcaa ataacataac taatgatttt cagcacgaag
    14401 ccaggatatt aaacagcata tggctcatta ccgctttagg tttggtgaca accgcaggac
    14461 tggcctgggt agcgaagtat ctcgaaatta cggcaacaaa atatgattca ccgcaaatgt
    14521 atgtcgccat aggattatta ttactttgta tgtatggctt aagtaaggat atcaacaaga
    14581 taaatgccgt catcgcaggc gtaatatatc tatttttact ctctttggtg gcgattgtcg
    14641 ttgctagttt agctcctgta cctgccatta tcgtcgtgtt cagcactgcg ggcgcgatgt
    14701 ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct caccgtttta
    14761 tcattatgat gacgttgaca gggttggccc tggtgatcat catgaatgcc gcattaatga
    14821 gtgaacgacc cgtttgggta ataagttgct taatgattgt gttatggtca ggcattatct
    14881 cgcatggacg aaataagctc cttgaattgg cggggaaatg ccatagtgaa gagttgtgga
    14941 gtccggttcg ttgcgctgtt acaggtgcat taacactcta ttactatttt atcggcttct
    15001 ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg cgtttttttc
    15061 attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc gcttcatttt
    15121 tcgcccgtag cttcttcgcc tggctttcca gtagcaggta acagatcagc gccagcagca
    15181 gcgggataaa gtaatacagg acgcgataag cgagcagggc ggcgataatg ctccctttcg
    15241 aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg atccccgccg
    15301 gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt acaaagaaat
    15361 agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc cagttaacgc
    15421 tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc ttttgccctt
    15481 tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc gcgataatca
    15541 tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag tgatccggca
    15601 actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc agccagttgg
    15661 tggtaatact gagcgagaaa atccgcgtaa tagtgctgcc cggtaaccct agtcgggagt
    15721 acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg ttgaaggcgt
    15781 agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg cagtaaaagc
    15841 gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc ccgaccgcac
    15901 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    15961 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16021 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16081 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16141 acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16201 cgccagcacg cttttggtca ggttccacca ggtgcgtttc ggcagcatgg tctcatcgac
    16261 ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct ggttaaaatg
    16321 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16381 actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt ggagcgggcg
    16441 gcggcggtac tcaaatacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16501 accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16561 gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16621 ggcgataatg acttctcgcc gcgcctgagt gagcattttc agatagtgac gttcaatatc
    16681 atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc ccggctggcg
    16741 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16801 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    16861 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    16921 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    16981 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cgaaatacca cgccagctgc
    17041 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17101 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17161 gtcatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17221 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17281 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17341 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17401 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17461 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17521 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17581 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17641 atcacatgaa tcgcttttcc ggtcatcggt ggcacaatgc ggcagtagag cacgccgcgc
    17701 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17761 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    17821 ctccacatcg tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    17881 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    17941 gtacgcaccg cgtcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaaacct
    18001 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18061 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18121 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18181 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac tggtctaagt tgctggcaga
    18241 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18301 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgttggtgt
    18361 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18421 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgt gtactggaaa
    18481 acaacttttt gctggcaatt gcagggctgg caatccttgc cggcccggcg gtagtgtctt
    18541 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18601 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18661 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18721 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18781 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    18841 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    18901 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    18961 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19021 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19081 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19141 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19201 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19261 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19321 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19381 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19441 tgagtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19501 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19561 agaatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgcgg ttcgcgcagc
    19621 agcgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19681 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19741 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19801 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    19861 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa aagcggcgtt
    19921 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    19981 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca gcgacccgcg
    20041 atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg ccagcatccc
    20101 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20161 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20221 cgcgccgatg accgtcatga taatggtcac cgcaccgggg ataatgaagt gctggctaat
    20281 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20341 gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat acccctgtac
    20401 aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg cggtggcgtt
    20461 ggcgcgctcc atctgttccg caaaatccac cggaataacc accagaccgc gaattttccc
    20521 cgcctgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt cgatgtaggg
    20581 cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt ccagtaaaat
    20641 cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa acagcagcaa
    20701 cagcgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct gccgcgtctc
    20761 tttgacgcac agcgcccgca cgcgacgcca ggaaaggatc gggttactca ttgctatgct
    20821 ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga tcgggttgct
    20881 catcgttagc cgactgtgct ttcaaatcgt ccggcgtgcc gctggcgatt aatttcccgc
    20941 ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg gtggtgacca
    21001 tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca cggcgggtga
    21061 gggggtcaac gccggaagtc ggttcgtcga gaaacagaat atccggttca tgcatcagcg
    21121 aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg gcgtgggagg
    21181 caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc tgcgcccgac
    21241 cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc aggttgccat
    21301 agagcgaaaa cttttgcgcc atatagccga gatgctggcg cgctttaccg gaactctctt
    21361 tcagatccat ccccagcacc agcgcctgac cggaggtcgg caccagcaag ccgcacatca
    21421 tcttaaaggt ggtcgatttc cccgcgccgt ttggcccgag cagaccaaaa atctcccctc
    21481 gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc ttggttagtt
    21541 ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat attgcaccca
    21601 gcggcgattc cgaggttccg gcaccgccca gcaaatcaat aaacgcatct tcaaaacgcg
    21661 gcgtagtttc gttgatgttg atttccggca tcccttcggc atggcgaata tcgtctggtg
    21721 tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg tcgctgacct
    21781 gtggcagttt caaggcgcgt tgcaatagtt tgcggttgcc ctcgtgtgga ctggtcatca
    21841 gaaaactgcg tccggccatg gtttgtgtca gggcttttgg ttccccctga tacagcaact
    21901 cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga tacgaggtac
    21961 tccagaggat taacatccct tcgcctgcca gctcatgcac catctgccac agttcgcgcc
    22021 gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc ggttcgccca
    22081 ccagggtaca ggccagaccg agtttttgtt tcatcccacc ggagagcttg cccgccaggc
    22141 gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg
    22201 cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc accgtgagat
    22261 cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc gcgccgtcgt
    22321 ttttgattgg atcaaagcca atcaccgtgg cattgccgct gtcgggtttc agtaatcccg
    22381 ccagcatccg cattagcgtg gttttacctg caccatccgg ccccaccagc cccgtcacat
    22441 aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg cccggaaagc
    22501 gtttttctag gccgttcagc gtgataacgg catcattcat gtcctgcctc gttaccgaat
    22561 tgcaccgtca ccggcattcc ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc
    22621 aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg ggtaaattca
    22681 gcagtcggcg aaacgaaacc aatctgtccg tgatagggct tgtccgggcg accatcggta
    22741 taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc aacataggca
    22801 cgtacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc attgagaacc
    22861 gtgcctggct ccaccgcgcg cgttaacagc gtgccatcag acggtgctat caacgttgag
    22921 tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc
    22981 gcctgagcga tgtcctgttc acggttaccg gaacggtact gacgcaattt atcctgtgct
    23041 gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag gtcatttgcc
    23101 gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg cgcatagtca
    23161 taggcggctt gagcctgttt caccgctgcg gcggcctgag cgatttcttc atcgcgatac
    23221 ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt cgcctgcatc
    23281 agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc tttgatagca
    23341 tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact aagatttacc
    23401 gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct ttgataccac
    23461 cagtagcctc cggcaaccac ggcggcaagt accactaccg ccaatctgat cacgacaggt
    23521 tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg atatgacagg
    23581 tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag ccggtacgta
    23641 acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac gcatgagtat
    23701 ggaggatcat gcgggtatcg ttggcgtcgc agccggtcca ggcggcaatc aggcgtgtca
    23761 ggtggctgtg tagcgggcta atcacctgtt cgtgcaccag gtgataggct gccgtgggag
    23821 agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg gtgagcagct
    23881 taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggctgcgcga tcaggctgtg
    23941 gttgtgcgaa caagcgttcg gcctcttcgg cgtgcggacg gaactgctcg ccaataaaat
    24001 cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga
    24061 tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg ttcattccat
    24121 attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt tcacccttga
    24181 ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt gattaagatt
    24241 atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc agtcacaggg
    24301 ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga cattgtggtt
    24361 tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt cggggcggtt
    24421 cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc gcgccgttgc
    24481 cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg cggtgctgga
    24541 aggccgcgac ctgatggcta gcgcccagac cggtaccggt aaaacagcgg gctttacgct
    24601 gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg
    24661 tgcgctcatt cttaccccga cccgtgaact ggctgcgcag attggcgaaa acgtccgtga
    24721 ttacagcaaa tatttgaaca ttcgttcgct ggtggtgttt ggcggtgtca gcattaaccc
    24781 gcaaatgatg aagctgcgcg gcggcgttga tgttctggtg gcaacaccgg gacgtttgct
    24841 ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga
    24901 agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt taacaaaact
    24961 gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata ttaaagccct
    25021 ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca ataccgcgtc
    25081 tgatcaggtg acccagcacg ttcactttgt tgataagaaa cgcaaacgcg aattgctgtc
    25141 gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta ccaaacacgg
    25201 cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgcgg cgatccacgg
    25261 gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg gtgacattcg
    25321 cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt tgccgcacgt
    25381 cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg ggcgtaccgg
    25441 tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac acaaactgct
    25501 gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc cgggctatga
    25561 gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac gtggcggcgg
    25621 cggtcgtggg caaggtggtg gtcgcggtca acagcaacca cgccgtgggg aaggtggcgc
    25681 aaaatctgca agcgcgaaac ctgcagaaaa accgtctcgc cgcctcggcg atgccaaacc
    25741 ggcaggcgaa caacaacgtc gccgccgtcc gcgtaaacct accgctgcgc agtaatcttt
    25801 tatgccgggc tatgcccggc atcaggctga tgaacaaacg caaaactgcc tgatgcgcta
    25861 cgcttatcag gcctacgtga actctgcaat atattgaatt tgcatgcttt tgtaggccgg
    25921 ataaggcgtt tacgccgcat ccggcatttc acaacaagca cttgtcagca atttgagaac
    25981 acgggaaaaa atttatctgt tttaccgccc ataagccacc gagacgttac ttctctatag
    26041 ccaattgttc gcgcaactcc attaaaaggt agcccagtcg attcttgccc ttaccattac
    26101 caccgtctcc ccagtaagca tcgttttctg tatgctcaac cagttttgcg ggcgcggttg
    26161 ccagcaagag cgcacgcagt tctggatgct gttcgaattt agcgcgaagt gcttttcgca
    26221 tcacctgttc tttgaccgac tcccagtttt tacgcagagg cttagaacga tcgcgcccca
    26281 tgcgtgcggc gaccataggg gaagaaaccc gacggatctc ttcgcggtat ttttcatcaa
    26341 ggaatttttg tgcctgaaaa tagtgttctg aggtgggcca ggttttcccg tcaaccttga
    26401 tgggccaggc ggcaaagtta gaaaaatcac cgtagtcatc gctggtgctg tagaaattta
    26461 tgatggtgtc ttgcatgacg tgttggatcc tttgtgctcg aacgggcatt aaaccgcatt
    26521 atgttggtgg ttattgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc
    26581 gaaaaatgcc acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc
    26641 gccgcgctta aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac
    26701 tttattcccc gtgcgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga
    26761 gaagaagggc ggcatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcctat
    26821 ttgatccccg gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc
    26881 aacgtggcgt tgcaggatca gatttacagc aaagatttac cgctgctgaa aaagatcatt
    26941 cccgatctta aattcactgc cgcttttggg cgtgggcgct acgtttgccc gcgtaatctg
    27001 acggcgctcg ccagcactga gcccacgcaa caggatctgc tggcgtttct tgacgacgaa
    27061 ctgacgccaa ataatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac
    27121 acttataaat gggatggtct gcgcgatcat actgatatcg ctatagatga cgatctctgg
    27181 cgtcgtttaa gtaccgacaa agccagctgc ctcaaccgca actgttacta ctatcgtgaa
    27241 tgcccgtttt ttgttgctcg tcgggagatt caggaagcgg aagtggtggt ggcaaaccat
    27301 gcgctggtga tggcggcgat ggaaagcgaa gccgtactac ctgacccgaa aaatctgctg
    27361 ctggtgctgg acgaaggtca tcacctgcca gatgtggccc gggatgcgct ggagatgagt
    27421 gccgaaatca ccgcgccctg gtatcgacta cagctggact tgttcacgaa actggtcgct
    27481 acctgcatgg agcagtttcg cccgaagacc atcccaccgc tggcgatccc tgaacgtttg
    27541 aatgcgcatt gtgaagagtt gtatgagctt atcgcctcgt taaacaacat tctcaatctc
    27601 tacatgcctg ccgggcagga ggcagagcac cgttttgcga tgggcgaact gcctgatgaa
    27661 gtgctggaga tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag
    27721 ttatttctta acgatttaag tgagaaaacc ggcagccacg atattgtgcg tctgcatcgg
    27781 ttgattttgc agatgaaccg cgcgttgggg atgttcgagg cgcaaagcaa actctggcgg
    27841 ctggcgtctc tggcgcaatc ttccggtgcg ccggtgacca aatgggcgac gcgggaagag
    27901 cgcgaagggc agctgcatct ctggtttcac tgcgtgggga tacgtgtcag cgatcagctg
    27961 gaaaggctgc tgtggcgcag tattccgcac attattgtta cctccgcaac cttgcgttcg
    28021 ctgaatagtt tttcgcgttt gcaggagatg agtggtctga aagagaaagc gggcgaccgt
    28081 tttgtggcgc tggattcccc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg
    28141 atgcgcgttg agccttccat cgacaacgaa gagcagcata ttgccgaaat ggcggccttt
    28201 ttccgtaagc aggtggagag caaaaaacat ctcggtatgt tggtactgtt tgccagcgga
    28261 cgggcgatgc agcgctttct cgactatgtg acggatttac gcctgatgct gctggtgcag
    28321 ggcgatcagc cgcgttatcg cttagttgaa ctgcaccgca aacgcgttgc caacggtgag
    28381 cgtagtgtgc tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg
    28441 ctcagccagg tgcatatcca caaaatcgct tttccgccca tcgacagccc ggtggtgatc
    28501 accgaagggg aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg
    28561 agcgcctcgt ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg
    28621 ggcgaagtgg ttatctacga caaacgcttg ctgaccaaaa attatggcaa gcgactactg
    28681 gatgcattac cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag
    28741 aaagaaaaaa cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag
    28801 tcgtaaatgg actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc
    28861 gatttagacc gggatactgc gcgcggtctg tatgcccata tgctcaatgg tgaagtccct
    28921 gaccttgagt tgggcggcgt attgattgcg ctacgtatca aaggggaagg ggaagcagag
    28981 atgctcggct tttacgaagc catgcaaaat cacaccatca agctgacgcc gccagcaggc
    29041 aagccgatgc cgattgtcat ccccagttac aacggcgcgc gtaaacaggc caacctgacg
    29101 ccgttgttgg cgattctcct gcacaaactc ggttttcctg tggtggttca cggggttagc
    29161 gaagatccaa cccgcgtgct gactgaaacc atttttgaat tgatgggtat tacgccaacg
    29221 ttgcacggcg ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgccagtc
    29281 ggcgcgtttt gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt
    29341 aacagtgcac ataccctggc gaaactggcg acgccatttg ccgaaggtga ggcgctgcgt
    29401 ctttccagcg tttcgcatcc ggaatacatt ggacgcgtcg cgaagttctt tagcgatatc
    29461 ggcgggcggg cgctactaat gcatggtact gaaggtgaag tgtatgctaa tccgcagcgc
    29521 tgcccgcaga tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac
    29581 cctgctggta gcgagttact gccacaagca aaagatccgg aaaccacggc gcagtggatt
    29641 gagcgttgcc ttgctggcag cgaaccgatt cccgaatcgc tgaaaatcca gatggcttgc
    29701 tgcctggtgg ctacgggtga agcggcaact atcagcgacg gcctggcgcg cgttaatcag
    29761 gcattttaat tcttttctca gccggatgag ccatgtcatc cggcttttac cccaccgcaa
    29821 tatgaaattc ctgcatcttt attgaccttc ccgcgcccgg cgtgcagcat aaaaaaacaa
    29881 caaacacata acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga
    29941 cgctgcacag ttttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga
    30001 aattagtggc cgatcattta atcgcggcaa acctggcagg gcatgattct catggtattg
    30061 gcatgatccc aagctatgtg cgttcctgga gtcaggggca tctgcaaatt aaccatcatg
    30121 ccaaaatcgt taaagaggcg ggggcggcag tcacgctcga tggcgatcgc gcattcggtc
    30181 aggtcgcggc acacgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg
    30241 ctgccgtggc gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt
    30301 gtgcagcggc ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg
    30361 caccgttcca cggtcgcgac agccgctttg gcaccaatcc gttctgtgtg gttttccctc
    30421 gtaaagataa tttcccgctg ttgcttgatt acgccaccag cgccattgca tttggcaaaa
    30481 cccgcgtcgc ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg
    30541 gcgtgccgac gaccaatccg gcggtaatgc aggagtcgcc gttgggttcg ctgttgacct
    30601 ttgccgaaca taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt
    30661 ccggcggtaa aacgacgcat caggaaacgt tacaaaccag tcccgatgcc attcttaact
    30721 gcatgaccac tatcatcatc aacccggaac tcttcggcgc gccggattgt agcgcgcaga
    30781 ccgaagcctt tgccgagtgg gtgaaagcct cgccgcatga tgatgataag ccgattttgc
    30841 taccgggcga gtgggaagtg aacacgcgtc gcgaacggca ggagcagggg attccattgg
    30901 atgcgggaag ctggcaggcc atttgtgatg cggcgcggca gattggtatg tcggaagaga
    30961 cgttgcaggc tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg
    31021 ggcaaacaag ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca
    31081 gacagggaaa tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat
    31141 tttgtaggcc tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcgt
    31201 aaacgcctta tccttcctac gaattactta taaattaccg cagtaccgct taatttattg
    31261 ttgttggtgg cggaagtgat gctgtatcca ctggcaccgg ctgccgcggc tttctcagcc
    31321 agtttcgctt ccagggcatc gagggtggat gcgccatcgg cagaaaccac gccgatttta
    31381 ttcatgttct gtgcctggga tgccgttacc ggttccgcgg caaacacgcc aaatgacaga
    31441 gttgaaagag ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag
    31501 gtgatttttg tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga
    31561 gagataaaat cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaacata
    31621 ctgataaacc atttatttgt tgtacttatg gacttgtttt atgcagcgtg gcagtcacga
    31681 cagcgttaaa ggcacggaat gccacgcaaa atacaaatta tactcgctat acatctcgcc
    31741 agttgtagtt aacgtaactg gctgtctttc gaacctctgc gattatatcc tgtatacgct
    31801 ggtttttgta aatctttctc ctgctgacaa tgaatgcata agcgcacgcc aggaatggct
    31861 tcccgacggg cttgcgggat gggggcaccg cactcttcac attcatccag gctttcgcca
    31921 cgcggaattt caccccgagc gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg
    31981 ttgacggcgt catcgttagc ccaaccggat gccatatcgt cctccccata tcaatgcttg
    32041 tacagttaag tgtagctaat ccagggacga acttgggcag ttcaagcatc agatctccga
    32101 ccattcccgc agcagattat gataaagatt aagcagcgac aggatctctt cattttcacc
    32161 atagcggctt ttcagcgact gaatattgtt gtccagttca aacagcatgg cgcgcttttt
    32221 atcatcacgg atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac
    32281 gggtgtcacg caatgcaggc tgctggaggg atacaacacg agatcgcctg ccgggagttt
    32341 tacccgatgt tgtccgaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg
    32401 atcgcttaaa aacagcgtgg cagaaaggtc agtacgcatc cagccgtttt gtggatggct
    32461 gcgtactgcg ccatccacat ggaaaccata ggtttcattg ttctgatagc gattaaacag
    32521 cggcgtggaa agggtacgcg gcaaggccgc ggcaaagaat aaagcatgtt ggttaaccgc
    32581 gttcagcacc tcattttgca gggcggcgta taacgtgctg cgggtgtcga cctgttgatt
    32641 gttcttaact tgcgcacctt gtgcgccggt ggtgacgcgt ccatccaccc attcggcttg
    32701 ttccagttgt tcgcgaaacc gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta
    32761 catcatacaa gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata
    32821 ttggctgtga gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttgttg
    32881 attgaggcga cgtaatcggt atcaaacagg ttgtaaacgt ttagctggaa gtcgagattg
    32941 cgattaaccc gataccccag tttggcatcg gcgacccagt aaccttcggt aaacgctggc
    33001 gttcccaccg cgccgtctga acctttatgc atactgccga tatagcgtgc gcccgcgcca
    33061 acagagatat catctgttgc ctgatactgg ctccataagg tgaaggcgtg ctccggggta
    33121 tacggcagcg atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt
    33181 tgctgggtat agccgccaat cacctgccac gcgggcgtga tattcccggc cacggatatc
    33241 tcatagcctt cgacacgttt cttaccgtat tgcgagtaag ttccgtcatc attttgctca
    33301 acttcatttt cgatatcagt acggaatagc gcggcggtga gcaacaggcg tttatccaga
    33361 acctgccatt tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg
    33421 ttggcactgt taccgctgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc
    33481 tgggaaacgg catagttaat atagacatta ccgttttccg tcaggtgata cagcgccccg
    33541 gctttccagt tcaccagatt gcccgacttg gcggtgtcga cggtggtgac cggagaacct
    33601 tttgccacac cagctgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca
    33661 ctgtcatatt cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg
    33721 gtgatttgca gcgtatcgaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt
    33781 gcgccgttgc gcgtcaggcc gccgggatga atgctgctgt caggatgata aatatttacc
    33841 gcgggtaacg tcaccggatt aacgccgtag ttagtctgcg tttcacgggt aaattccacg
    33901 ccggtactga catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg
    33961 ttggtgagaa ttttattact cacatctttg gtattcgccg tgcgtgacca ggtccagcta
    34021 ttcacatcgc tggtgggctg agtaatattc gacgccccgc ccataatcgc cgtcatcagg
    34081 taatcctgct ttacgcgcga ccaacgggta gtattgcgaa tggtggtgtt atcgttgatg
    34141 tcgtgctcaa aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg
    34201 ccgtaaaagt tatgagtatc aacttttccg gaatgattca gagccgctgt tcccgcagat
    34261 ggggcagaat aacccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctgg
    34321 gtgacatgca gataattaag atacaaacga ttcgctgtac caaggccaaa agcgatagaa
    34381 ggggcgacgc cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg cgttttttcg
    34441 cccatcacat tcaggcgcac cgcagtggta tcaccaatga cctgattgac gtccagcgtg
    34501 ccgcggcgga accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc
    34561 ggctgcttgc tgatcatatt gatcgagccc gtcggcgcgc tgcgcccgta gtcggtgccg
    34621 gaaggccctt taatcacttc gacctgctca gtattgaagg tgtcgcgcga gacgctgccg
    34681 atatcgcgaa tgccatcaat ataaatactg ttagaggtat cagcaccgcg catataaatg
    34741 gcgtcgccag tggtggagtt accgttctca cccgcaaaaa acgcacccac gccggggacg
    34801 tttttgagcg cgtcggtaag gttggttgcg ccctgatctt taatcacttg ttcagaaatc
    34861 accgtcatcg tgcgggtagt atccgctacc ggacgcgaga atttcggatc ggcagattgt
    34921 tgtggcgcat aaagcgaagg cgttgatgct tcgacaacca gcgtgtcatc cgcgttagtt
    34981 tgcccttcgg cggcgagtgc ctgagccaca ggcgtgatgc caatacaaag accggcaaag
    35041 aacgtgagcg aatgaaattg tctggcaggg aaattgcgat tgttttccat tttgcaggtg
    35101 acttttttta tatgttgaaa ttttcactgc cacgagaaag cctctctgcg gatgcgatgc
    35161 cactgttaat ctcgtgatag tgttcgtgca agtgataata attttgataa gcattatcac
    35221 ttggcgcgat tttctatcac ccattgcgcg aaaaagaaat acatttattt acattgcgtt
    35281 catatttgat gttttttgat tacaaaacag gcgaaaactg catccagagg cgaatgcagt
    35341 tacgttggtg gggttatttg tagatggttg cagtaccgaa catctggtca tttccgcctg
    35401 cggaattaat gacataacct ttcgcgcctt gctcgcgcgc tttttctgcc agtttatcct
    35461 ccagatcgct taggtttgat gcgccagttg ccgaaacggt accggcgggc cgtagctgac
    35521 cgctgtccag gttactcatc ggttgcgcgg cataagccgt gagagagatt ccgctcagga
    35581 cggtggcaat cagtagtgtg aggcactttt tcataatgac atcctcataa atacaacggg
    35641 tagtgcctga taagtgtagg tcgcctgggc gatgaaaatg acgcaaaaaa ttgacagaga
    35701 tgtgcttatt ttttgaacga tatattttta caaaataaga caaatcaatg acatgcataa
    35761 tgctgatttt tatgatgaat tgccataaac catagccatg ttaaggtttt ggggaatgat
    35821 aaacgcagcc agatatttac cctgtcgcgt tatgcgggta tcttacgccg ctgcttaaag
    35881 gagaatgcta tgtccgccca gaaaccgggg ttgcatccgc gcaaccgtca tcacagccgc
    35941 tacgatctcg ccacgctttg tcaggtcaat cctgaactca ggcaattcct cacgcttaca
    36001 cccgcagggg agcaaagcgt agactttgcc aatccgctgg cggtgaaggc gctcaataag
    36061 gcattgctgg cccattttta cgccgtagcg aactgggata tcccggacgg ttttctctgc
    36121 ccgccagtac cgggccgggc ggattatatt catcaccttg ccgatttact ggcagaagca
    36181 agcggaacaa ttccagctaa tgccagcatt ctggatatcg gcgttggtgc gaactgtatt
    36241 tatccgctga ttggcgtaca tgaatatggc tggcgtttta ccggtagtga aaccagcagt
    36301 caggcgttaa gtagtgcgca ggcgatcatc agtgctaatc cggggcttaa ccgcgccatt
    36361 cgtctgcgtc ggcaaaaaga gagtggggcg atttttaacg gcatcatcca taaaaacgag
    36421 caatacgacg cgaccttgtg taacccgcca ttccacgatt ccgccgctgc ggcacgggca
    36481 ggtagtgagc gtaaacgccg taacctgggg ctgaacaaag acgatgcact gaactttggc
    36541 ggccagcaac aggagttgtg gtgtgaaggt ggtgaagtcg cctttatcaa aaagatgatt
    36601 gaagagagca aaggcttcgc gaagcaggtg atgtggttta cctcgctggt ttctcgtggt
    36661 gaaaacttac cgccgttgta tcgtgctctg acggacgtgg gcgcggtgaa ggtggttaaa
    36721 aaagagatgg cccaggggca aaagcagagt cgctttattg cctggacctt tatgaacgac
    36781 gagcagcgcc gccgttttgt taatcgccaa cgttaaagcg ttggctcccc cggcggcagc
    36841 ggttcagccg gggccgcgcc cggagcaggc agcacctgat aagtctgcac tggcgcgcga
    36901 acacctgcca ggtcgaaatg ctttttcacc tggctgtcga gggcaaagcg gaccgtccac
    36961 tgtttgagtg gcagcgtggt gaacgaaaca cgcagtgtaa acgcggtatt gcttaagccg
    37021 acaatcccgg caaagtttgg ttcaccaata accagcccgc gaatttcttc gttttccatc
    37081 aattccgcta ccgcatcttt cagtgcctga ttggctttat cagcatcttc atggcgatca
    37141 acatcgtagt ttgccaccac cgaaccaatg ccgcggacga agttagcaaa ggtggttatc
    37201 gaagaccaag gaatgatgtg atacgccccg gtatcctgac gcacgcccac ggagcgaatc
    37261 gacatccgtt ccacagtgcc ggtcaacggc ccgatagtaa ccaaatctcc agtgttcatg
    37321 ccgttttcaa actgaataaa taccccggtg ataatatctt tcaccagcgt ttgcgaacca
    37381 aacgagatag ccagacctaa tgccccagca cctgccagca atggcgcgat attgacgccg
    37441 atttccgaca acacaatcat gatggtgatg gtactgataa tcaccgccag cgcgttacgg
    37501 aacagcgtca gcagggtacg cgtacgggcg ctgggtagcg ggcggccatg aatatccgaa
    37561 gccagccggt tttcgatcaa actggcgagc actgtccagc caaccgccga gaagaaaaga
    37621 atgagtgcga tgcggatcag gatatctacg gttttctgcc ccgcgccgtt ttgcagccag
    37681 ttccagaaat cgaacaatcc ccatgcgctc aacagcaaca ttaccgccac gcagacggtc
    37741 agaatacgcg ccgttttcag cgccgccgaa agccagccat tcagccgttt ttgtagctcc
    37801 ggatagttac gctgagtatg tggcgagagg gtgatggttt tcgccagcca gcgggaaaac
    37861 ataccggaaa caaacgctgc gataccaata atcgccaggc tgcgcaccgt tgcacccatc
    37921 ataaatttca ggctgttgcc cggatcgaac aacgaaaaga aaaacagcac gataaaatag
    37981 gcgcttgcca gccagtgcca caccagcgca aaggcgcgga taaacaggct gaaaaaggcc
    38041 agcgaatgct ccgcaaagtt gagcaaatgt tgggtaatct cttttttatt gcgaaagatc
    38101 aggtacaacg cccagacggt catgcacagc ataatgatga cgttcgccag cgcacctatc
    38161 tgtacattca cctgattaga gataatcggc acggccacaa tcaggccata acctatcaaa
    38221 ctgcttaacc agctcaggcg acgactccag taacgggcgc tctcgtcctg aatcgtgaac
    38281 gggcgcagtt cagccacgtt tgggcaaaaa atcaggcgta gtacggcttt gaaaaattca
    38341 atcagggcaa aggcgttgag aaacaaactt tgttggaaag cgatggtgcg actgcccgca
    38401 ttcaggttgt cgcttaatac ctggccgaca aacaatgtca gtgccagtaa cagtaggtcg
    38461 ataataaacg ccccgataat catcgccgga agctgcagcc agttgctgcg ttcgcgattt
    38521 ttttgccgcg cccactggcc cattttgcga tacagcggca gtgcgcacag acgaatcagc
    38581 cagtaaaaac caaacactaa taccgctaac attgaaaaat gggtcaacgc attgctgaag
    38641 gtttgtggat taaacggctt atgcggggag ccggtgatat tgcgataaag ttgtccgaag
    38701 cgggcggaaa gggcttcacc ataatggcgg ctgacttcgg tgaccttttg cagcacggtt
    38761 tgctcttcaa ccagcgtcgg cggcacgatc ttcgggaccg gttccgcagg gggcgtcgcg
    38821 gcgacagtac gcaactggtc gatcaattct ttacgcgagg tgtcgttatc cagcacatcc
    38881 gccagtgcgc cataggccgc ttttttttgt tcgatatccg gttccggggc cggttcagtc
    38941 gttgagtccg ttgtcgttgt ggttgtaacg ccgggtatgg ataccgcgtg ggcaggtgcg
    39001 cccagcaggc agaagaggat gaacaggatc caccgcatga ctcctccagt gagaaaacag
    39061 agcaaaaaga taagtataga tgctggggga ggggcgattt taaatgagag gaatctggtg
    39121 tgcctccctt tcgggtgaaa gggaggcaga gaagttaaga gacgtgctgc aaaaattcct
    39181 gcaagcgctg gctcggcggg ttcttgatca atacctgcgg atcgccatct tccgcaatcc
    39241 ggcctttgtc gataaagatc agccgcgaag ctactttctc ggcaaaaccg atttcgtggg
    39301 tcacgatcac catcgtcatc ccttcttcag ccagatcctg cataaccttc agcacttcat
    39361 ggcgcagttc cgggtcaaga gcggaagtcg gttcatcaaa cagcatcatt ttcggcttca
    39421 ccgccagcgc gcgggcaatc gccacacgct gctgttgacc accagaaagt tcggaagggt
    39481 agtgatgtgc acgttctgcc agaccgactt tcgccagcag ctcacgcgcc agtttttccg
    39541 cctcttcttt gttcgcgcca cgcacgcgta gcggaccaaa catgacgttt tccagcgccg
    39601 tcagatgcgg gaagaggtaa aactgctgga acaccatacc tgcttcctgg cgaatcaggc
    39661 gctcgtcaac tttcggatcg ttaaccttca ggccatcgac aatcagatcg ccggaggtga
    39721 tttcttccag tttgttgatg cagcgcagca gggtcgattt accggaaccg gacggcccga
    39781 taatcaccac gacttcgccc tgggcaatgt tcaaatcgat attgtgcagc acctgggttg
    39841 ggccaaagtg cttggagacg tttttaaatt caatcacagg attttcatcc ttctttccag
    39901 acgacgcaga ataaagctca gcaccagggt aataatcaga tagaacaccg ccacggcgct
    39961 ccagatctca agggcgcgga agttaccggc aataatttct tgcccctgac gggtcagttc
    40021 cgccacgccg atcacaataa acagcgaggt gtctttaatg ctgatgatcc actggttacc
    40081 cagcggcggc agcatacgac gcagtgccag cggtaaaatg acgtagcgaa tggtttccca
    40141 acgtgaaaga ccgagcgcca gtcctgcttc acgaaaacct ttgtggatag acagcaccgc
    40201 accacgcgtg atttccgcaa tgtacgcgcc ggagttgatc atgatggtga ccaccgccgc
    40261 agtaaatggg tcgatgcgta agtcgttaaa cgccatcggc agggcgaaat agataaacat
    40321 cacctggacg acgataggtg tgccgcggat cacttcaata aagaccagcg cgacgtggtt
    40381 ggctatccaa ccaccgaagg tgcgtgcaaa acctgccagc aatccgatta ccagaccgcc
    40441 tgccagaccg aggaccgaaa tccacagggt cattttggca ccttcaatca gaagcggaat
    40501 ggcaggccag atggcactcc agtcaaactg catatgttgt tcctgttacc gtggtgtaaa
    40561 taatcaaatt cagggtaatt aatggccccg gagcgggttt gcgcccctca ccctaatcct
    40621 ctccccatag gggagaggga actgccagtg cgttttacag gtgtagcgtt attatttcgg
    40681 ttcagtaccg aaccattttt tgtagatttc gttgtaagtt ccgttctcgc gcagggtttt
    40741 caacgcgccg ttgactttgt cacgcagctc gtcgctacct ttcgggaacg caataccgta
    40801 ttgctgcgct tccagagagt cacctaccgc tttgaactga ccgttaccgg cggttttgat
    40861 gaagtacaga atatttggcg tatcgtgcag aacggcgtct gcgcggttgg tgcccagttc
    40921 catataggcg ttatcgatgt tcgggaactg acgcagatct ttagttttga tgtttgcttt
    40981 cgcgtaatca acggagccag taccgctctt cacagcaacc actttcccgt cgagatcttt
    41041 cacgcttttc acatcgttat tgttagcttt caccatcact aacaggccgc ttttgtagta
    41101 gccgtcagag aaatcgatcg cttttttacg ctcgtcggtg atggtaatgc ccgccagcgc
    41161 cagatcgacg tttttggttt gcagtgccgg aatgatccca ctgaaatcca tcggcttcag
    41221 ttcgtaatcc agcttcagct ctttagcgat ggcagcccac agatcaacgt caaagcccac
    41281 atatttatcg ccctgtttaa attcaaacgg aacgaaggcg gtatccgtcg cgacaactaa
    41341 ttttttatcc gcggcatgag aagaaaccgc aaaagccagg gtcagtgcag ccagtgaaac
    41401 ttttaataca gacttcatag catttccttt tttctatcca cgggacgatc ccctgcgtga
    41461 cattcacata tatgaaaaaa tcgtgccagt tttgcaactc cttgattttt cgagatgcgg
    41521 caaaaacgtg atgcacgatt tatagggcaa taccctgaag atgcaccatt ctggggcacc
    41581 aatctggtgc gctaaaattg tgcactcaac acagtgatta tttagcgtaa aacctgatga
    41641 aaaaacaatc tttatgtaac gattgtgtga tgatgtggat acaaaaaatt tacttaatca
    41701 gctggagata gcagatggat gcactaaata agtgcgttga ggtgggctgc aaaacaaaac
    41761 ggcctcctgt caggaagccg cttttatcgg gtactaaagt tctgcaccat cagcgatgga
    41821 tttattcgat gttagactcg ataaaccaca ggaatttatc caggtcgcga gacgcggcgg
    41881 tcaggatatc tgcggtgtcg tcatctttcg cttcgccaat cgctttgcgt acgtcattag
    41941 cgacgattgc gtaacggtca gccagttctt tcaggtgatc ctgaacgttg tggatgtcca
    42001 gcgggtaact tttcagcggg gttttgctgt tgataacttg agtggtcccc agagctacac
    42061 cgcccagctg cactgcacgt tctgccatgg tatccagatg atcgatcagt gcggtgcgga
    42121 agccatccag catttcatgt acggcaatga agttagcgcc gcgcatgttc cagtgcgctt
    42181 gtttggtaat caaagaaaga tcaataaact ggataacctg gcgattcagc aactctactg
    42241 ttgctttttt ctcgctgtcg gagacatcgt tgcgggtata aagcagattg gtcgcttttg
    42301 atttaactaa tttagcggta ctcataattt catatcctct tgatgttatg tcccagtaat
    42361 taacgagact aagtatagca ccggctatgt gttccgctat tctggctgtt cctatcacac
    42421 taatagtggt aacaagcgtg aaaaacaaaa ctaataaaga ttcaatgagt tagatatatt
    42481 gataagaaca attcttattt atctgctcgc caggaatttt cggaataaat atgcttttgc
    42541 ataattcgat atataaagac ggtgtagagg aaaagtagcg agaaattctg catggttatg
    42601 cataaccatg cagaaatgta aatttaatta atgtctaatt cttttatttt gctctctttg
    42661 cgtactgtca gcgtagaccc cattgaagcg gcgatgatag cgccgagcgc cagcagctgt
    42721 atgggtgtca gtgtttctcc gaggaaaatc atcccggaaa cggcagccag cgccggttcc
    42781 atgctcatca gcgtaccaaa tgtccgtgtt ggcaaacggg tgagggcaat catttccagc
    42841 gaataaggca gagcggtcga gagaatagcg acagccagac ccaatggaat aaccgaccag
    42901 tgccagagtg cttcaccagc ctgaagcgct ccaattggca cgaaaattaa cgctgcaatc
    42961 aacgaaccaa ttgccaccgt cgcagggcca tgttccgctc ctgcgcgttg cccacttaaa
    43021 atgtaaatag cccaacaagc cccggccccc agtgccagcg cacagccggt taaatcgaca
    43081 tgggaaacgt cttgccccag cggtagcagg aaccacagac caagaaccgc cagcacaacc
    43141 cagacgaaat ctaccgggcg acgagaagag aacagcgcca ccgccagtgg tccggtgaac
    43201 tccagcgcca ccgcaatacc cagcggtact gtctgaatag aaagataaaa aagataattc
    43261 atcccaccca gcgaaacgcc gtaaaacaac agcggtaacc gttgctcttt ggcaaagcgc
    43321 agtcgccatg gcttaaagaa cgcgatgagg atcagcgttc ctaatgccag acgcagcgca
    43381 gtgacacccg gtgcgcccac cagaggaaaa agtgacttag ctaacgaggc tccaccctga
    43441 atagacgcca tggcaacgag caatatgatt attggtaacc agaccggcat tttacgtaat
    43501 gaaccaggca tcctttctcc cacaaatatc tagacttaag taaagcgtgg agtgtactgg
    43561 atatacccaa tgctggttga gcatttgttg aaaaaatttt cccccgtttt gactaaaatg
    43621 cgccaggatt gatggaatca ttagtctggt gattaggaat aatctggatg aatgacaggg
    43681 aaaacatgcg taatacttac gcagttctct gaaaaagtga tttaaattta gatggatagc
    43741 ggtgtatgga aacgttctgt tacatgaaat ggcccgttag acatcacaaa tcgcgaagag
    43801 tttcccatta atttttgata tatttaaaac ttaggactta tttgaatcac atttgaggtg
    43861 gttatgaaaa aaattgcatg tctttcagca ctggccgcag ttctggcttt caccgcaggt
    43921 acttccgtag ctgcgacttc taccgtaact ggcggttacg cacagagcga cgctcagggc
    43981 caaatgaaca aaatgggcgg tttcaacctg aaataccgct atgaagaaga caacagcccg
    44041 ctgggtgtga tcggttcttt cacttacacc gagaaaagcc gtactgcaag ctctggtgac
    44101 tacaacaaaa accagtacta cggcatcact gctggtccgg cttaccgcat taacgactgg
    44161 gcaagcatct acggtgtagt gggtgtgggt tatggtaaat tccagaccac tgaatacccg
    44221 acctacaaac acgacaccag cgactacggt ttctcctacg gtgctggtct gcagttcaac
    44281 ccgatggaaa acgttgctct ggacttctct tacgagcaga gccgtattcg tagcgttgac
    44341 gtaggcacct ggattgccgg tgttggttac cgcttctaat cactttggtg atataaaaaa
    44401 tccgcctctc ggggcggatt tttgttttta aggcttcggg tcgaaaatat cggttccgag
    44461 atggttgtaa tccaccttct gtaactggaa gttggtgatg tatatcgacc cggctttctt
    44521 ctcagatata aacgggtatt tgatgttaat ttccttcgct ttaattcccg tccactggga
    44581 gaaaaaacct aagaagtcat tggctgagcg gcgggcttta atcacccgat gcgctttatc
    44641 gtcgctggaa atgaccataa aaggcacctg gaaattttgc tgatacttat catcatgggc
    44701 aaggtattgc acgtctttgc cgcgctcttt aaaggccaga ccgtggtcag aaaagtaaac
    44761 cagcgagaag ctgctgccgc tgttgcgtaa ctgatcgtac agcttgcgca gtaaatcgtc
    44821 cgtttgcgtc atggtataga gatagcacga cgtttctttc gactgcacaa aggtttcgta
    44881 tttgccttgt gtcctgtcgc aggcctgcgg atgtgagccc atcagatgta gaacaatcag
    44941 ctgcggttgc gagtgctctt gcgccagcac ttgagcggtc atatccagta acgcttcgtc
    45001 tttggtattt ttatctgctt caaaattacc ttctttcagg aagtacactt catctgctcg
    45061 tttggcgatg ctggcgatag cggtatcgta ttcgccgatt tgaccctggt tggaaaacca
    45121 ccaggtctgg aagcccgcgc gatttgccag ggtgacaaag ttatcctgaa actgtggttt
    45181 gccatcgact acgcgattga gcgttaagcc aagcgatttc tgcgtggagc cactggcggc
    45241 aatgtagtca gcaaatatca aaccgttaac gctgctggca aacggggtat tgtcccagtg
    45301 accgccaaag gcaccgaggg catcgcgacg cgcgctttca ccgatcacca ccacataggt
    45361 ctgatacttc ggcttaacgg cagtgaccgt ccaggtatct ttcattccgg aaagttttgc
    45421 catccgttct tgctcttcaa tcacctcgtt attattgacg atgacatcct tagcaaaacg
    45481 aaataccgga tagccagtat ctttcagttt aaatacgccg ccccaggcca ggttttgcac
    45541 gggagcaaca aaaaatgtcg ccacactgaa taccagacac aggctgtcga aggtattcca
    45601 gcgcgctttt tcactctctt tttttcgcct gattgctatt acgccgagca caaaaataaa
    45661 tatgccgacc atgtagctgt accacggaaa aatcgtcagg atttcggtcg actcttccat
    45721 attggtggag tgcaatgcca gcaatgtatt gaaattaggc gcgccgtagg cctgagcaaa
    45781 agggaaataa caagccgcca ccagcgaact gacaccgacc agaacttttt gtacgcgagg
    45841 caatgttcgc catagcaaaa gcaaaatagc cgtaaacgca gcggtgtaga gcaaactgaa
    45901 ggggtaacct aagccgaggt taattaatag cgactgtaaa aagtagaacg cagtccacgg
    45961 gctaaatacc cggctacggg taacaagcga ttctttgagg gttaaattca tatgccacta
    46021 tcgtaacaaa cgccatgtgc ttaccctggc gtcaagggtc aatacctgca agagtgcgtg
    46081 aagagtggaa aggggtccgc atccgcgagc cgcaatatac gcagggctgc aagaagatag
    46141 agcgagccgc cgttcaggtc aactactgac gaacaaatct tttgcgaagg ggattgcaaa
    46201 tccgacaaaa agtggggttt ttcagaccaa aacgcgttat gcctgcatga aagcaggagt
    46261 gaagcggcgt accgcgacgc cgctatttat cggaaggttt atcttgctgc ggtttgttgt
    46321 tgaccatatc gcacaacata gagagcagca ttaaccgtac tttaaaggga gaatgactaa
    46381 acacgcgcat acacctcttg aactcattca taagacctcc tgacttgcta atcccgtcga
    46441 tccttgaggg atgattgcat tacatacaga tatagcacag gctatattat atagctattg
    46501 ctaaaacgtt aattttttgt accttcgcaa ctctggttta caatgtgcgc acgaaatgag
    46561 aacgctatgt atgcgtcacc ataatgagga agcacaatga gtcgtcgcgc aggtacgcca
    46621 acagcaaaaa aagtgacgca gttagtgaac gtggaagagc acgttgaagg gttccgccag
    46681 gtcagagagg cgcatcggcg cgagcttatt gatgattacg ttgagctgat ttctgacttg
    46741 atcagggaag tgggggaagc tcgtcaggtg gacatggctg ctcgtctggg agtttcgcaa
    46801 ccgacggtgg ctaaaatgct taagcggctg gcaaccatgg ggctgattga aatgatcccc
    46861 tggcgtggcg tgtttttaac ggcagaagga gagaagctgg cgcaggaaag ccgcgagcga
    46921 catcagatag tcgaaaattt cttgttggtg ttgggcgtca gtccggaaat cgcccgtcgc
    46981 gacgcggaag gcatggagca ccatgttagt gaagagacgc tcgacgcttt tcgtttgttt
    47041 acccagaaac acggtgccaa atgagcctgc cttttttacg cacgctgcaa ggcgatcgtt
    47101 tttttcagtt attaattctt gttggtatcg gattaagctt tttcgtgccc tttgcaccga
    47161 aatcctggcc tgctgctatc gactggcaca ccatcatcac cttaagcggc ctgatgctgc
    47221 tgaccaaagg tgtggagtta agcggttatt ttgatgtgct ggggcgcaaa atggtgcgcc
    47281 gctttgctac ggagcgtcgg ctggcgatgt ttatggtgct ggcggcggcg ctgctttcta
    47341 cctttctgac caacgatgtc gcgctgttta ttgttgttcc gctgactatc acgctaaaaa
    47401 gactgtgtga gatcccggtt aatcggctga ttatttttga ggcgctggca gtcaacgctg
    47461 gttcgctact gacgccaatt ggcaacccgc aaaatattct tatctgggga cgttctggtc
    47521 tttcgtttgc cggatttatt gcccaaatgg caccgctggc tggcgcaatg atgctgacgc
    47581 tcctgctcct gtgctggtgt tgtttccctg gaaaggcgct gcaataccat acgggggtgc
    47641 aaacaccgga gtggaaaccg cggctggtgt ggagttgtct ggggctgtat atcgtctttc
    47701 tgacggcgct ggagttcaaa caagagctgt ggggactggt gattgtggcg gcaggctttg
    47761 cgctgctggc acgtcgcgtg gtgctcagtg tggactggac gctgctgctg gtgtttatgg
    47821 cgatgtttat cgacgtccat ttactgaccc agcttccagc gttgcaaggc gtgttgggta
    47881 acgtgagtca tctatctgaa cccgggttat ggttaacggc aatcggttta tcgcaggtga
    47941 tcagtaacgt gccgagtacc atattgttgc tgaactatgt gccgccgtct ttattactgg
    48001 tatgggcggt aaacgtaggt ggctttgggt tattacccgg atcgctggca aatttgattg
    48061 cgctacgtat ggcgaacgat cgccgcatct ggtggcgttt ccatctctat tcaataccga
    48121 tgctgttgtg tgcggcgttg gtgggatatg ttttgttagt tatactcccg gccaactagg
    48181 gtctggcaaa taaaaaaggc ggattatgag tccgcctttt gctttaagtt ttgaagatta
    48241 attcagacga accggcatcc cggagcggtt tttaatcgct tcatcaagaa caacctggtc
    48301 aacatctggc tgaccggtca ctgtttgcac gctcttcgtc agggtaattg gcacaatttc
    48361 ctgaccttca aactgggctt cggtggtaga cagcgggtta tggacttcaa tataacggct
    48421 gccgtctggc tcggtggtcg cttttaccgg ctcatcaata aactgtacgc gggtaccgac
    48481 cggtactttc tcgaacagga atttgatgtc ttcgttacgc agacgcacac aaccatgact
    48541 tacacgcagg ccgataccga agttggcgtt ggtgccatgg atagcgtaca ggcgaccgat
    48601 gtagagtgca tacagcccca tcgggttatc cggacctgcc ggaacgacag ccggaagcgg
    48661 ttcgcccgca gcgcggtact ctgcgtgcat tttggcggtc ggcgtccagg tcgggcctgc
    48721 tttcttacgc tcaactttag tggtccagtt gataggcgta tctttgccta actgaccaat
    48781 gccgatcggc agcacgataa cggtgttggt ccctttcgga tagtaataca gacgcatctc
    48841 tgcactgtta atgacgatgc cttcatgaac ggtatccggc aggatcagct gctgcggaat
    48901 gttcagtaca gtaccgcctt tcggcaggaa ggtatccaca cccgggttcg cttccatcat
    48961 attggaaagc cccatctggt actccgcggc aaaatactcc agcggctgag tgttaccttc
    49021 aggaatggtg atcacctgat tctgaccaac caggcgactc ccgtcggttg gcagaggata
    49081 agttaccgca gaggcggtac tgcaaaagcc gacaacagcg aaggccgctg cgaataatgt
    49141 tttcaatttc atattcatgt taagcgagat tttgtgcctg gcaggccatt gggttgagaa
    49201 tattagagta ttggaagcgc attataagtt cattccagct cacagtgaaa tcagatgtgt
    49261 acgaaatcac attttttgcc tttggcttga gtgtagacct taagcgagga gcaggatctt
    49321 ctttcagact tatggcataa tgcgcggttt gtcatatctc ttttcaggat acgcctgtgt
    49381 tagtttccag taacgtcacc atgcagttcg gcagtaagcc gttgtttgaa aacatttccg
    49441 tcaaatttgg cggcggcaac cgttacggcc tgattggcgc gaacggtagt ggtaaatcca
    49501 cctttatgaa gatcctcggc ggcgacttag agccgacttt gggtaacgtt tccctcgatc
    49561 ccaacgagcg cattggtaag ctgcgtcagg atcagtttgc ctttgaagag ttcactgtgc
    49621 tggatacggt gatcatgggg cataaagagt tgtgggaagt gaagcaggag cgtgaccgca
    49681 tctatgcttt gccggaaatg agtgaggaag acggctataa agtggccgat ctggaagtta
    49741 aatacggcga aatggacggt tactctgcgg aagctcgcgc cggtgaattg ttgcttggcg
    49801 tgggaattcc agtggaacag cactacggcc cgatgagtga agttgctcct ggctggaagc
    49861 tgcgtgtgct tctggcgcag gcgctgtttg ctgatccgga tattctcctg ctcgacgaac
    49921 cgaccaataa cctcgacatc gacaccattc gctggctgga acaggtgctg aacgagcgtg
    49981 acagcaccat gatcatcatc tcgcacgacc gtcacttcct taacatggtt tgtacccaca
    50041 tggcggatct ggattacggc gagctgcgcg tttatccggg caactacgat gagtacatga
    50101 cggcggcgac ccaggcgcgt gaacgtctgc tggctgataa cgccaagaag aaagcgcaga
    50161 ttgctgagct gcaatctttc gttagccgct ttagcgccaa cgcctcgaaa tctcgccagg
    50221 caacttcgcg cgcgcgccag attgataaaa tcaaactgga agaggtgaaa gcctccagcc
    50281 gtcagaaccc gttcatccgt tttgaacagg ataagaaact gttccgtaac gcgctggaag
    50341 tggaaggtct gaccaaaggg tttgataacg gtccactgtt taaaaatctc aacttgctgc
    50401 tggaggtcgg tgaaaaactt gcggtactgg gtaccaacgg cgtcggtaaa tcaacgctgc
    50461 tgaaaacgct ggtgggcgat ctgcaaccgg acagcggcac cgtaaaatgg tctgaaaacg
    50521 cgcgcattgg gtactatgct caggaccacg aatatgagtt tgaaaatgat ctgaccgtgt
    50581 tcgaatggat gagccagtgg aagcaggaag gcgatgacga gcaggcggta cgcagcattc
    50641 tcggtcgttt gctgttcagc caggacgaca tcaaaaagcc agctaaagtg ctttccggtg
    50701 gggaaaaagg gcggatgctg tttggtaagt taatgatgca gaagccgaac attctgatca
    50761 tggacgaacc gaccaaccac ctggatatgg aatccatcga gtcactgaac atggcgctgg
    50821 aactgtatca gggcacgctg atctttgttt cacacgaccg tgagttcgta agctccctgg
    50881 cgacccgcat tctggaaatc accccggaac gcgtgatcga ctttagcggt aattacgaag
    50941 attacctgcg tagtaaaggg atcgagtaag tgattatccg tcaccccgat actgtcgggg
    51001 tgacggcggc tgatgacaaa tgcaaaatag cctgatgcgc tacgcttatc agtatctacg
    51061 cagtacctgt aatatattga atctgcagga ctttgtaggc cagataagcg tagcgcatca
    51121 ggcattaaga aagcgcactt gtcagcaatc tgagtaacgt cgaatcgatt ttcttaatcc
    51181 cacatcaatt cacttccatt aactctcaaa catccatgcc caacgctcgc ttaccgtgaa
    51241 tgttgaggtc ggcaagcgta aagcgtcctt cccagtttgt ttcatagtcc tcgcgcggga
    51301 agtcgcccgg cgatgcgcct ttttccagtg cggccttcac cttgtgcgcg taggcaagat
    51361 ttttctcgca catcggtgcc gcaggaatgt acatcacgtt gccccaaccc tgttgatttt
    51421 caacgggggc aacggaatgg atgacgtcgc agtgccacca gacggagtct ccggcctcga
    51481 gttttggaat gctggttaac gcctcaatta acagtggatg ccattgctct gataccggca
    51541 acactcttcc gggcgctacg ccgcacagtt catcctccgg cacatcatca agcagcggac
    51601 gtaacagtac gtacgccatg gcttcaggaa tgggcacaac gtgcagcaac ccctgaccag
    51661 gcagcatatc agagagcgct gtccagccct ggaatgtccg aaacacggaa catttggtgg
    51721 tgttgtccac cgtgtactct tcaacttccg tacgatgtgc cgcatgccag ggatcatatt
    51781 gcgccagatt gccattaaag acgttggcga aaacgcgctg atacgctgga agcagccagc
    51841 gttccagcgc cccggagtcg gtatgcgctc caagaccttt ggaggtcgtt ccgggcggac
    51901 ggcggcggat acggtcaggg tagatgacgc tcacatccgg gttaaaccat tgctttccat
    51961 cactttcaaa tgtccacaga cgattgagaa acgactgcgc attcgccatt tcttcactct
    52021 ggcgggcctg catttgcgcc tgcgaccagt agatggggta aatctcggga cgtgaagcgc
    52081 tgagcgtccc gaagaaatta tcgccggggc ctttgtagac ctcgtcaaag cggttgcggt
    52141 ccagatagtc cagcatcgac tgatcccagc ctagcgcttg ttcgcggggg aaatggcctt
    52201 ttatcaccgc acaaccgcga cgtttaatct gttcgcgctg ctctgcagta acatgacctg
    52261 ctttgatatc ggcataagac agcaccggcc agacggcatc gccctgtgct ttgagtgcgt
    52321 tgatttcagc cactcgcgtg gcaatgtcat cgcttagctg attaaagatc tgctggacgt
    52381 cgccaagctg cgcccgcagc gcgtgcttca tctgacggat agctgctttg tgatcggcag
    52441 gcaatgtgtc gctggtaaaa gtagaagcca taaccacctc tcattaactt tcattcgaaa
    52501 ccattctgat ttgcatgtga caatataagt tataaataag ttaatgcaag tttaatgatt
    52561 tgatgtgatg tacaggatgt aaaaagaagg gaaaaccgga gcgcacggct ccggtagaga
    52621 ggtcagctgt taaaagggga tgtgttatcc agcaccgcct gaatcacatt cagcgcgcct
    52681 tcatgattat tatcatcggt agcgtaacgg gcgatttgtt taatgttttc cgcagcattg
    52741 cccatcgcaa aggaataacg cgccattttc agcatctccg catcgttacc gctgtcgcca
    52801 atcgctacca cattttgcgg tgacagatcc cagcgtttca gtaaccgcga aataccgttt
    52861 gctttatgta gaccgggaat aatcaggtcg ataaagccaa aaccactggt gacgggtttc
    52921 ataatgccat cgagcgctat gtgcagtttg tcgatcacta acgggatttg ttcatctggc
    52981 aggttgagcg aaaacttgaa cagtacgtcg tcaatctcct gataatcttt tacagctttc
    53041 aggcgatggt agtgttttgc catcagtgcg acaaatgctt cgggggcatt ttcgctgaca
    53101 tatgcacttt gcagaccgca ggcgacaaaa ttgagttgct tatcttttag caactcgcca
    53161 ataacaatcc gcgattcatg tcgggtcagt tcgccgtgga acagttgctt gccatgttcg
    53221 taaaccagtg cgccgttttc cgcgacaaaa gagatctcat cctttagctc aggaaagaac
    53281 gaaataagct ggtaatactg attaccgctg gcgacaacga acttaatgcc gcgctttttc
    53341 agttcctgat attgcgccat aaaatgtggt tggttgtacg ttttggcgtc gttaagaaaa
    53401 gtaccgtcca tgtctgtgac gataactttt acgctcatag gtgtgctcct ggctcgaaaa
    53461 tgaaaccgta acagtgtaat aacaatgtga cgaagagcac aaattatatt tcgaatgaaa
    53521 gtaaggatga aattgatgat gtgaatgatt tagcccggcg acgacgccgc cgggccgagg
    53581 agattacagc atatgttcag tacgggcgat gatatcgtcc tgtgcgtctg gagagagcgc
    53641 ggtgaagaag gcggaatagc ccgcgacacg cactaccaga tcgcggtact gatccggatg
    53701 ttttttcgcc tccagcagcg tttcgcggga aacgatgttg tactgaatat gccagccttt
    53761 atgcacttca aagaaggtgc gcagcaggat catcagtttc tgcttgtcag attcgttctc
    53821 cagcgttgcc ggattcagtt tctggttgag caacacgccg ccgagaatcg ctgccgtagg
    53881 cagtttaccc accgagccaa tgaccgcagt agggccgaga tggtcagtac cagaggccgg
    53941 actagcgcct tctgccagcg gggtgtgggc tttacgcccg tccggtgttg ccatagtttg
    54001 cgcgccaaac ggtacgttag cggagataga cgaggtaccc gcgtaatagt tgccgccaac
    54061 cggaccacga ccgtagcgcg ggttatggta ctgtttcagt tcgtcgatat aggtctgata
    54121 agcgcgagcc agcagcgtat cgacggtatc atcgtcgttg ccgtacttcg gcgcaccgtt
    54181 aatcagccgc tgacgcagtt gctcatgagt caggccatcg aagtcatccg ccagtgcggc
    54241 agccagctgt tgctgaccaa tcgcgccctg ttcaaatacc agtttcttca ctgccgccag
    54301 gctgttgccg aggttggcaa tgccaacctg caggccagaa acccagtcat atttcgcgcc
    54361 gccttgcttg atacttttcg ctcgctcaat acagtcatcc accagcgccg agcagagaat
    54421 atcgtgtacg ttctcttcca gcatggtgtc gacgacatat tcgatttcga ttgatttacg
    54481 cgtgtaataa cggatttgcg tatcccacgc gtccattact tcatcgaaat tgttaaagtt
    54541 gcctgccgac aacgcttttt cttgtggcag gaacactttg ccgctggtgg catcacgccc
    54601 gccttccagc gccgccagca tcacgcgggc gaagttgata aagctcatgc cggtacagcg
    54661 atagccccat ttgccaccga cggcggtttc gatacaacca atcgctgcgt agtcgtaagc
    54721 gtcctgcggt tcaataccga gtttaataaa ttccgggatc acgatttcgt cgttgttgaa
    54781 cgccggcatc ccgaagccgc aacggatcac ctgtacgcag gcgtcgagga aatcattact
    54841 cattcctgca tggtaacgca cgctgaggtt aggctgagtg gaacgcaggc gaccgcagga
    54901 ttcgaggatc gcgtaagaga ggggattcac tgcatccatt ggttgaccat caaccagatt
    54961 ttgtccgcca atggtaacgt tctgatacag cggacttccc gcagaggctt ttgagtgtga
    55021 gccggagcgg atcttgttca cttccagcag tttcagccag cagctatgca gcatctcgat
    55081 ggcgtgttcg cgatccagcg tctggttgag ttcaacgtcg cggcgatagt acgggtagag
    55141 atactggtcc atacgaccaa acgataccga gtgaccgtta gattcgatct gcaaaatcaa
    55201 ttggatgaag taacacagtt gcagcgcctg ccagaaagtc tgcggcggct ggtgggcgat
    55261 aagatcgcag ttttctgcca tcgtcagcag ttcatcgcga cggctttcgc gagtttcggt
    55321 agcagccatt tcacgcgcca gggcagcgaa acgttcaatg tgttcactga ctgccaccag
    55381 cacgatatca atcgctttca ggaattgttc gccatgtaaa tcttccagca ccgtcagatt
    55441 gatgcgcgag cgacgttccg ctactttctc acgcagacca tcaagacctt tttccagcag
    55501 cagcgggaaa ttcaccgcca ggtgcgcatc gccggaggtc atattgcctt ccgctttaat
    55561 gattccggtc gccagcagac ctttttgctc atcggtaaac atgccgtagc agcgatcctg
    55621 tacggtctga ccgcgccacc acgggcacac ttcatgcaga acgcgtttgt tctcttcgct
    55681 caccgcaaag ccagcaccgg gacgatctgc cagatcatca atctcttttt cgatccacga
    55741 gacagtatat tccgggaaga tcggcgcggc gcgaacttcg cttgcctggt tgccaatgat
    55801 caactcatcg tgtttgatcc agatggtgcg attcgccagg tgatgcgcca gtgccagcgc
    55861 gcgacgtacc gggatcggct tatcgagatg ttgttgatac atctcggtat agtgctgcgc
    55921 gcgctcggta cagactggcg gtttcacaat atgcaccagc gcatttttgt gcgctttaat
    55981 gcggtcgctg agcgtgtcca gtttcagtgt ggtcatggtt gttatcctcg taaggtcgcg
    56041 gttaaccctt tctggcaggc atactgctgg gcaaagtcga gcagttctgg cgcatcaagc
    56101 ggtttttccg gggcgtcata gggcagatta agtaagtgat atttgttgat gcccagcgtg
    56161 tggtagggca gaaaatgaat ttcgccaacg tgcagctcgt cggcggcaaa atcggtaatg
    56221 gcttttacag aggtttcgtc ggcattaaag ccctgaatca gcggcacgcg gataatgatt
    56281 tttttgcccg ctgcggcgag ttttttcagg ttatccagca ctctggcggc gttaccgtcg
    56341 gtccactgtt taaacggcgc gtcggcaacg tgttttaaat cggcaagaaa cagatcgata
    56401 tagggcagag aaggggcgat atatttccac ggcacatgca gacaggtttc taccgcagta
    56461 tgaatgcctg cctcgtggct ggcttgcagt agcgccatcg ccatttccgg ctgcataaag
    56521 ggctcaccac ccgaaagcgt taaaccgccg ccgctgcgat cgtaaaacgg tttatcgcgc
    56581 agaacggtcg tcatgatctc ctcaacgctt ttcacttcac cacacacggt taatgcctgt
    56641 gtcggacagc agtcggttaa cgccgtcaga tgctccgggg ttaacttttc ccgatgaata
    56701 agcaaaccat tcagcgcgcg ctcaatcact tccggcgcgg ccttagcgca cagctcgcag
    56761 ccttccagac acagtcgtgc gtcatacagc agatcctgcg tgcgggcgcg gctttccggg
    56821 ttctgacacc agcggcagcc cagcgaacag cctttaagaa atacgaccgt gcggataccg
    56881 gggccatcat gggtcgagta gcgctgaata ttgaaaatca tagttgcctc tctgtttcgt
    56941 tcaaacatta aaatgctttc gaatgaaagt tagattgatg tgcgtcaact gttcagagag
    57001 ttttcccgtg atagtctaca ttcagacaaa aagtacattt tgaggatggt tatggaactg
    57061 tatctggata cttcagacgt tgttgcggtg aaggcgctgt cacgtatttt tccgctggcg
    57121 ggtgtgacca ctaacccaag cattatcgcc gcgggtaaaa aaccgctgga tgttgtgctt
    57181 ccgcaacttc atgaagcgat gggcggtcag gggcgtctgt ttgcccaggt aatggctacc
    57241 actgctgaag ggatggtgaa tgacgcacgt aaattgcgtt ctattattgc ggatatcgtg
    57301 gtgaaagttc cggtgaccgc cgaggggctg gcagctatta agatgttaaa agcggaaggg
    57361 attccaacgc tgggaaccgc ggtatatggc gcagcacaag ggctgctgtc ggcgctggca
    57421 ggtgcggaat atgttgcgcc ttacgttaat cgtattgatg ctcagggcgg tagcggcatt
    57481 cagactgtga ccgacttaca ccagttattg aaaatgcatg cgccgcaggc gaaagtgctg
    57541 gcagcgagtt tcaaaacccc gcgtcaggcg ctggactgct tactggcagg atgtgaatca
    57601 attacgctgc cactggatgt ggcacaacag atgattagct atccggcggt ggaagccgct
    57661 gtggcgaagt ttgaacagga ctggctggga gcgtttggca gaacgtcgat ttaaccggac
    57721 gttctgcatc ctcataaatt gctgatgacg tggcggagtg ccgcgtctta tctggcctgg
    57781 aggtggcaat tactgcccgc acacctcaca ccccggattg cgcatcagtt tcatttcgcg
    57841 aaactgacag gtcatcgcat cgtacatgac gattttcccg ctggccggtt tgccataacc
    57901 tgccagcagt ttgatcgctt ccattgcctg caacgaacca attacgccga tcaacggtgc
    57961 catcacgcct gcttctacgc aggttaatgc gttctcgcca aacaaacggc taaggcagcg
    58021 atagcacggt tcgccgtcct gataagtgaa gaccgtgatt tgcccttcca tacgaattgc
    58081 cgcgccggaa accagcggca ccttcgcggc aaaacagcca gcgttcagtt gattacgcac
    58141 cgcaacgtta tccgtacagt cgagtaccag atcgtgtttt gcaatcatcg cagcaagttc
    58201 agcgtcatca agcagtgcat tgactggcgt aatcgcgata tgtgggttga tccgcgtcat
    58261 ggcgtcacgg gcggattcta ccttcggttg cccgaccgta gcatcactgt gcagcgtctg
    58321 gcgttgcaga ttcgagagcg aaaccgtgtc gaagtcgagc agcgtcaggt tgccgacacc
    58381 ggcgcttgcc agatactgcg aagctgcgca gccgagtcca ccgaggccga ctaccagcac
    58441 acgagaatct tttagcgcct cctgaccgtc aaaatcaaaa ccgcgcagaa tgatttgccg
    58501 gttgtagcgc agcatctcct gatcgctgag ttccgccatt acaggcctcc gaacaacgcg
    58561 ttaaacggtt ctacttccac ccattcgccc acgtccacat tgccgcgatc gcgttccagc
    58621 acgataaagc agttgccgag actaaaggag ctaaatatat gtgaaccctg atgtccggtg
    58681 gtcgtcactt caagttcacc gtcggcgttg cgttgcagca cgccgcgctg gaaatcaaga
    58741 cgtcctggcg tttttttcag gcgggacgct gtgcgtacgc gctggcgcgc gggcaggccg
    58801 ctggcggtgt tgccgcttag ttttgccagc aaaggctgta ccagttgata gaaggtcagc
    58861 gtcgctgaaa ccgggttgcc cggcaggccg cagaaccagc tattgctgag tttaccgaac
    58921 gcgaacggtt tacctggttt aatcgccagc ttccagaagg cgatctcccc cagctcttca
    58981 agaatcgttt tggtgtaatc cgcttcaccc actgaaacac cgccggaact gatcactaca
    59041 tccgcctggc tgtcggcttc aataaatgcg gcgcgcaggg catggggatc gtcgcggata
    59101 atccctaagt tgattacctc gcaacccagc tgttctaaca tcaggtgtac ggcgagacgg
    59161 ttggtgtcgt agatttggcc gtcgcccagc ggctgaccgg gcaactggag ttcatcaccg
    59221 gtagaaaaaa gcgctacacg cactttacga atcaccggaa cttcggcaat ccccagtgaa
    59281 gcaattactg gcagctctgc ggtagtcagg cgagttcccg ccgggaaaac aaccgcacct
    59341 gcagagatat cttcaccgcg acggcgaata ttttgcccgc tacgcacttc agcagtaaaa
    59401 cgcacgccat tgtccgtttg ttcagtctgc tcctgcatca ccaccgcttc gcagccttcc
    59461 ggcaccggcg caccggtcat aatacgaatg caggtgcccg caggccattc accatggtac
    59521 ggctgaccgg caaaggattt accggcaacg ggcagcggtt gcctggaggc aatatcggct
    59581 aaacgcaccg cgtagccgtc cattgcggag ttatcaaacc ccggaacatc aagcggcgaa
    59641 acgacattgc tcgccagaat acgaccaaaa cactgtacca gtggcagcgt ttcctgggcg
    59701 gtcagtgggg tgacgcgaga aagcatctca ttaagcgcgg tgtcgagcga catcaatccg
    59761 gtggtaaatt ccatgaaaac actcctgcgg aggcaaaatc gaatttgcct attatgtcag
    59821 aaaaacgcca cagactgtat gccacctcgg gcgtagcgtt ggctcctgcc tttacatgcc
    59881 atatccatct ttctatattc aaaaattgaa tgagtaattc ataaaaattc tgatatttat
    59941 agcaaaagtg gcgagccacc attaatggac gaatactatg ggcaaagcag tcattgcaat
    60001 tcatggtggc gcaggtgcaa ttagccgcgc gcagatgagt ctgcaacagg aattacgcta
    60061 cattgaggcg ttgtctgcca ttgttgaaac cgggcagaaa atgctggaag cgggcgaaag
    60121 tgcgctggat gtggtgacgg aagcggtgcg tctgctggaa gagtgtccac tgtttaacgc
    60181 cggaattggc gctgtcttta cgcgtgatga aacccatgaa ctggacgcct gtgtgatgga
    60241 tggtaacacc ctgaaagccg gtgcggtggc gggcgttagt catctgcgta atccggttct
    60301 tgccgcccgg ctggtgatgg agcaaagccc gcatgtgatg atgattggcg aaggggcaga
    60361 aaattttgcg tttgctcgtg gcatggagcg cgtctcgccg gagattttct ccacgccttt
    60421 gcgttatgaa caactactgg cagcgcgcaa ggaaggggca accgtcctcg accatagcgg
    60481 tgcgccactg gatgaaaaac agaaaatggg caccgtgggg gccgtggcgt tggatttaga
    60541 cggcaatttg gcggcagcca cgtccacagg cggaatgacc aataaattac ccggacgagt
    60601 tggcgatagt cccttagtgg gtgccggatg ctacgccaat aacgccagtg tggcggtttc
    60661 ttgtaccggc acgggcgaag tcttcatccg cgcgctggcg gcatatgaca tcgccgcgtt
    60721 aatggattac ggcggattaa gtctcgcgga agcctgcgag cgggtagtaa tggaaaaact
    60781 ccctgcgctt ggcggtagcg gtggcttaat cgctatcgac catgaaggga atgtcgcgct
    60841 accgtttaac accgaaggaa tgtatcgcgc ctggggctac gcaggcgata cgccaaccac
    60901 cggtatctac cgtgaaaaag gggacaccgt tgccacacag tgatgaactt gatgccggta
    60961 atgtgctggc ggttgaaaat ctgaatattg cctttatgca ggaccagcag aaaatagctg
    61021 cggtccgcaa tctctctttt agtctgcaac gcggtgagac gctggcaatt gttggcgaat
    61081 ccggctccgg taagtcagtg actgcgttgg cattgatgcg cctgttggaa caggcgggcg
    61141 gtttagtaca gtgcgataaa atgctgttgc agcggcgcag tcgcgaagtg attgaactta
    61201 gcgagcagag cgctgcacaa atgcgccatg ttcgcggtgc ggatatggcg atgatatttc
    61261 aggagccgat gacatcgctg aacccggtat ttactgtggg tgaacagatt gccgaatcaa
    61321 ttcgtctgca tcagaacgcc agtcgtgaag aagcgatggt cgaggcgaag cggatgctgg
    61381 atcaggtacg cattcctgag gcacaaacca ttctttcacg ttatccgcat caactctctg
    61441 gcgggatgcg ccagcgagtg atgattgcga tggcgctgtc atgccgcccg gcggtgctga
    61501 ttgccgatga gccaaccacc gcgctggatg tcactattca ggcgcagatc ctgcaattaa
    61561 tcaaagtatt gcaaaaagag atgtcgatgg gcgttatctt tatcactcac gatatgggcg
    61621 tggtggcaga gattgccgat cgggtactgg tgatgtatca gggcgaggcg gtggaaacgg
    61681 gtaccgtcga acagattttt catgcaccgc aacatcctta cacccgtgcg ctgttagctg
    61741 ctgttccgca acttggtgcg atgaaagggt tagattatcc ccgacgtttc ccgttgatat
    61801 cgcttgaaca tccagcgaaa caggcccccc ccatcgagca gaaaacggtg gtggatggcg
    61861 aacctgtttt acgagtgcgt aatcttgtca cccgtttccc tttgcgcagc ggtttgttga
    61921 atcgcgtaac gcgggaagtg catgccgttg agaaagtcag ttttgatctc tggcctggcg
    61981 aaacgctatc gctggtgggc gagtctggca gcggtaaatc cactaccggg cgggcgttgc
    62041 tgcgcctggt cgaatcgcag ggcggcgaaa ttatctttaa cggtcagaga atcgatacct
    62101 tgtcacccgg caaacttcag gcattgcgcc gggatattca gtttattttt caggaccctt
    62161 acgcttcgct ggacccacgt cagaccatcg gtgattcgat tatcgaaccg ctgcgtgtac
    62221 acggtttatt gccaggtaaa gacgcggctg cacgcgttgc gtggttgctg gagcgcgtgg
    62281 gcctgttacc tgaacatgcc tggcgttacc cgcatgagtt ttccggcggt cagcgccagc
    62341 gcatctgcat tgctcgcgcg ttggcattga atccaaaagt gatcattgcc gacgaagccg
    62401 tttcggcgct ggatgtttct attcgcgggc agattatcaa cttgttgctc gatctccagc
    62461 gtgatttcgg cattgcgtat ctgtttatct cccacgatat ggccgtggta gagcggatta
    62521 gtcatcgtgt ggcggtgatg tatctcggac aaattgttga aattggtcca cggcgcgcgg
    62581 tcttcgaaaa cccgcagcat ccttatacgc gtaaattact ggcggcagtt ccggtcgctg
    62641 aaccgtcccg acaacgaccg cagcgtgtac tgctgtcgga cgatcttccc agcaatattc
    62701 atctgcgtgg cgaagaggtg gcagccgtct cgttgcaatg cgtcgggccg gggcattacg
    62761 tcgcacaacc acaatcagaa tacgcattca tgcgtagata acattcaggc ggagaataaa
    62821 atggcaagag ctgtacaccg tagtgggtta gtggcgttgg gcattgcgac agcgttgatg
    62881 gcatcttgtg cattcgctgc caaagatgtg gtggtggcgg taggatcgaa cttcaccacg
    62941 ctcgatccgt atgacgcaaa tgacacgtta tctcaggccg tagcgaaatc gttttaccag
    63001 gggctgtttg gtctggataa agagatgaaa ctgaaaaacg tgctggcgga gagttatacc
    63061 gtttccgatg acggccttac atacaccgtg aaattgcggg aaggaattaa attccaggat
    63121 ggcaccgatt tcaacgcagc ggcggtaaaa gcgaatctgg accgggccag cgatccggcg
    63181 aatcatctta aacgctataa cctgtataag aatattgcca aaacggaggc gatcgatcca
    63241 acaacagtaa agattactct caaacagccg ttctcagcgt ttattaatat tcttgcccat
    63301 ccggcgaccg cgatgatttc accggcagcg ctggaaaaat atggcaagga gattggtttt
    63361 catccggtgg gaaccggacc gtatgaactg gatacctgga atcagaccga ttttgtgaag
    63421 gtgaaaaaat tcgcgggtta ctggcagcca ggattgccca aactggacag cataacctgg
    63481 cgtccggtgg cggataacaa cacccgcgcg gcaatgctgc aaaccggtga agcgcagttt
    63541 gctttcccca ttccctacga gcaggccaca ctgctggaga aaaacaaaaa tatcgagttg
    63601 atggccagtc cgtcaattat gcagcgttat atcagtatga acgtgacgca aaagccgttc
    63661 gataacccga aggtccgtga ggcgctgaat tacgccatta accgtccggc gctggtgaaa
    63721 gttgcctttg cgggctatgc aacgccagct actggtgtgg taccgccaag tatcgcctac
    63781 gcgcaaagtt ataaaccgtg gccttacgat ccagtgaaag cgcgcgaatt actgaaagag
    63841 gcgggatatc ccaacggttt cagtaccacg ctgtggtcgt cacataacca cagcaccgcg
    63901 cagaaagtgc tgcaatttac ccagcagcag ttagcgcagg tcgggattaa agcccaggtg
    63961 actgcgatgg atgccggaca gcgggcggca gaagttgaag gtaaagggca aaaagagagc
    64021 ggcgtgcgga tgttctacac tggctggtcg gcttcaaccg gcgaagccga ctgggcacta
    64081 tcgccgttgt ttgcctcgca aaactggcca ccgacgctgt ttaataccgc gttttacagc
    64141 aataaacagg tggatgactt cctggctcag gcactgaaaa ctaatgatcc ggcggaaaag
    64201 acccgtttat ataaggcggc gcaggatatc atctggcaag agtcgccgtg gatcccgctg
    64261 gtggtagaaa aactggtgtc ggcacacagt aaaaacctga ccggtttttg gatcatgcca
    64321 gacaccggct tcagctttga agatgcggat ttgcaataag caacgcaggg agtggaatgc
    64381 ttaattacgt tatcaaacga ttactggggt tgattccgac gctgtttatc gtctcggtgc
    64441 tggtgttttt atttgtccat atgctgcccg gcgatccggc gcgattgatt gccgggcccg
    64501 aagctgatgc gcaggttata gaactggtgc gtcagcagct ggggttggat cagccgctgt
    64561 atcaccagtt ctggcactat atcagcaatg ctgtgcaggg ggattttggc ctgtccatgg
    64621 tgtcgcgtcg tccagttgcc gatgagattg ccagccgctt tatgccaacg ctgtggctga
    64681 ccataaccag tatggtctgg gcggtgatat ttggtatggc ggcgggaatt atcgccgccg
    64741 tctggcgtaa ccgttggccg gatcgattga gtatgaccat tgcggtgtcg gggatctcgt
    64801 ttccggcatt tgctctgggg atgcttttaa ttcaggtatt ctccgttgaa ctgggctggc
    64861 tgcctactgt gggagcagac agttggcagc actacatttt accctccctg acgctcggcg
    64921 cggcagtggc cgccgtgatg gcgcgcttta cccgtgcgtc atttgtcgac gttttaagcg
    64981 aagattatat gcgtaccgcg agggcgaaag gggtgagcga aacctgggtt gtcctcaaac
    65041 acgggctacg caacgcgatg atcccggtag tgaccatgat gggcttacag tttggctttt
    65101 tgctcggtgg atctatcgtg gtggagaaag ttttcaactg gcccggactt ggacgcttac
    65161 tcgttgactc cgtagaaatg cgcgattacc cggtgattca ggcggaaatt ctgcttttct
    65221 cgctggaatt tattcttatc aacttagtgg tggatgtgct ttacgccgcc attaacccgg
    65281 ctatcaggta caagtaagga tgcgactatt taactggcga cgtcaggcgg tgttaaacgc
    65341 catgccactg gtcaaacctg accaggtgcg tacaccgtgg catgaattct ggcgacgatt
    65401 tcgccgtcag catatggcga tgaccgccgc attattcgtt attttattga ttgtggtggc
    65461 catttttgca cgctggatcg ctccctatga cgccgaaaat tattttgatt atgacaacct
    65521 gaataacgga ccttctttgc agcactggtt tggcgtcgat tcactggggc gcgacatttt
    65581 cagccgtgtc ctggttggtg cgcaaatctc gctggcggcg ggcgtgtttg ccgtgtttat
    65641 cggtgcggcg atcgggacgt tgctgggctt gttggctgga tattatgaag gctggtggga
    65701 tcggttgatc atgcgcattt gcgatgtgct gtttgccttc ccgggtattt tactggcgat
    65761 cgctgttgtt gcggtgttgg gaagcggcat tgctaacgtg attatcgcag tcgccatttt
    65821 ttccatcccc gcgtttgccc gactggtgcg cggcaacacg ctggtgttga aacagcaaac
    65881 ctttattgag tcagcacgca gtattggtgc cagcgatatg accattttat tgcgtcatat
    65941 cttgcctggg accgtctcgt ctatcgtggt gtttttcacc atgcgcattg gcacctcgat
    66001 tatctctgcc gccagcctgt catttctcgg cctcggtgcg cagccgccga caccagagtg
    66061 gggagcaatg ctcaatgagg cacgagcgga tatggttatc gcgccgcatg tcgctgtttt
    66121 tccggccctg gctatttttc tgaccgtact ggcgttcaat ttgttgggcg atggtttacg
    66181 cgatgcgctg gatccgaaaa ttaaaggata gttacgtttg aatattgcgt gaaagggtaa
    66241 tcacctcaca ggaaattatt gccctaagca agtgttgtaa ctttctgctg attttgtaga
    66301 atcgggtaat ttggttaaaa agccgcagca agggactatt tttgcagcgg cacagcgttc
    66361 agatagttat tttgttaaat gtattaacat gctgagttta tacgaaaaga taaagataag
    66421 gctgataatt ttatttttat tggcagcact gtcatttatt ggtctttttt tcatcattaa
    66481 ctatcaactg gtatcggaac gcgcggtaaa acgtgccgat agtcgctttg aacttattca
    66541 gaaaaacgtt ggctatttct ttaaagatat tgaacgttcg gccctgacat taaaggactc
    66601 actgtattta ttaaaaaata cagaggagat tcaacgcgcc gtgattctga aaatggaaat
    66661 gatgccattt ttagactcgg tgggactggt acttgatgat aataaatatt atctcttttc
    66721 gcggagggcg aatgataaaa tcgttgttta tcatcaggaa caagtaaatg gaccgcttgt
    66781 cgacgagtca gggcgggtta tttttgccga ttttaaccca tcgaaacgac cgtggtcggt
    66841 ggcttcagat gactctaaca acagctggaa tccggcatac aattgctttg atcgtccggg
    66901 taaaaaatgt atctctttta cgctacgtat caacggcaaa gatcacgatt tgttagcggt
    66961 agataaaatt catgtcgatt taaactggcg gtatctgaac gagtatcttg atcaaatcag
    67021 cgctaatgat gaagttctat ttttgaaaca aggtcatgag atcattgcca agaatcaact
    67081 cgctcgtgaa aaactgatta tttataatag cgaaggtaat tataatatta ttgattctgt
    67141 cgatacggag tatattgcaa agacatcagc agtgccaaat aatgcattgt tcgaaatcta
    67201 tttttattat cctggcggta atttattgaa cgcatcagat aaactttttt atctaccgtt
    67261 tgctttcatt attatcgtgt tgctggtggt atatttcatg accactcgtg tgttccgtcg
    67321 gcaattttct gaaatgaccg agctggttaa tacgctggcg tttttgcccg actcaacgga
    67381 tcaaatcgag gctctgaaaa ttcgcgaagg cgatgcgaaa gagattatca gcatcaaaaa
    67441 ttcgatcgcg gaaatgaaag atgccgaaat tgaacggtca aataaattgc tctcactgat
    67501 ctcttacgat caggaaagcg gttttattaa aaatatggcg attattgagt ccaacaataa
    67561 tcagtatctg gctgtgggga tcatcaaact gtgtggtctg gaagccgtgg aagcggtgtt
    67621 tggtgttgat gaacgcaata aaatcgtcag gaaattgtgt cagcgaattg ccgagaaata
    67681 tgcgcaatgc tgcgatatcg tgacattcaa tgccgatctc tatttacttc tgtgtcggga
    67741 aaatgtacag acatttaccc gtaaaatagc gatggtaaac gattttgaca gcagctttgg
    67801 ctaccgcaat ctgcgcatcc ataagtctgc catttgtgaa cctttgcagg gggaaaacgc
    67861 ctggagttac gcagaaaaac tgaaactggc gatttccagt atccgtgacc atatgttctc
    67921 cgagtttatt ttttgtgatg acgcgaaact caacgaaata gaagagaata tctggattgc
    67981 gcgtaatatt cgccatgcaa tggaaattgg cgaactattc ctcgtctatc aaccgatcgt
    68041 tgatattaac acccgcgcca ttctgggcgc ggaggcgttg tgccgttggg tgtctgcgga
    68101 gcgggggatc atttcaccgc tgaagttcat taccattgct gaagatatcg ggtttatcaa
    68161 tgagctgggt tatcagatta ttaaaacggc aatgggtgaa tttagacatt ttagtcagcg
    68221 tgcgtcgctg aaggatgatt tcttactgca tattaatgtt tcgccctggc agttaaacga
    68281 accgcacttt catgagcgtt ttaccaccat catgaaagaa aatggcctga aggcgaacag
    68341 cctctgtgtt gagatcactg aaaccgtgat cgagcgaatt aatgaacatt tttatctcaa
    68401 tattgaacaa ctgcgtaaac aaggggtacg gatatcgatt gatgactttg gcaccggttt
    68461 gtcaaacctg aaacgttttt atgaaattaa tccagatagc ataaaagtgg actcgcaatt
    68521 taccggcgat attttcggta ctgcgggaaa aattgtgcgc attattttcg atctggcgcg
    68581 ctataaccgg atcccggtga ttgcggaagg cgtagagagc gaagacgttg cgcgcgaatt
    68641 aatcaaatta ggatgtgttc aggcccaagg gtatctgtac cagaaaccca tgccattctc
    68701 cgcctgggat aaaagtggaa aattagtaaa agagtagttt acgtatgtcc agaatcaata
    68761 agttcgtact tacagtcagt ctgctgattt ttatcatgat ttcagcagtt gcctgcggga
    68821 tctacactca aatggtaaag gaacgggtgt atagcctgaa acagtccgtt attgatactg
    68881 cttttgcggt ggcaaatatt gctgaatatc ggcgtagcgt ggcaattgat cttatcaata
    68941 cgctaaatcc cacggaggaa cagctgttgg ttggcttacg cacagcttac gctgactcgg
    69001 tttcaccctc ttacttgtac gatgtcggtc cttatctgat ttccagtgac gaatgtattc
    69061 aggtaaagga gttcgagaaa aattattgtg cagatattat gcaggttgtg aagtatcgac
    69121 atgtcaaaaa tacagggttt atctcttttg acggtaaaac cttcgtctat tacctctatc
    69181 cggtaactca caatcgtagt ctgatatttt tgcttggtct ggagcgtttt tctttactgt
    69241 caaaatcgct ggcgatggac agcgagaacc tgatgttctc tctgtttaag aacggtaaac
    69301 cggtgaccgg tgatgaatat aatgctaaaa acgccatctt caccgtttcg gaagcgatgg
    69361 agcacttcgc ctatttgccg accggattgt atgtatttgc gtataaaaaa gatgtttatt
    69421 tgcgggtttg tacattgatt attttctttg ccgcattggt ggcagtgata tcgggtgcca
    69481 gttgcctcta tctggtacgc agagtgatta atcgtggtat tgtggagaaa gaagccatca
    69541 ttaataacca ttttgaacgc gtactggatg gcgggctttt cttttcggct gccgatgtca
    69601 aaaaactcta cagtatgtat aactcggcgt tcctggacga cctgaccaaa gcaatgggca
    69661 gaaaatcctt tgacgaagat ttaaaagcgc tgccggaaaa aggcggttat ttgtgcctgt
    69721 ttgacgtcga taaattcaaa aacattaacg acaccttcgg tcatttgctg ggcgatgaag
    69781 tgttgatgaa agtggtgaaa atccttaaat cacagatccc ggtagataaa ggtaaagtct
    69841 accgcttcgg cggtgacgaa tttgcggtga tttatacggg tggaacgctg gaagagttgc
    69901 tatcgattct aaaagaaatc gttcatttcc aggtgggaag cattaattta agtaccagta
    69961 tcggtgtagc acattcaaat gaatgtccta ccgtcgaacg cttgaaaatg ctggcggatg
    70021 agcggctgta taagagtaag aaaaacggca gggcacagat tagctggcag taatcattat
    70081 tcgcaggccg gacaaatgat tttgcccggc ctgaattaat taaacccggc taccccacaa
    70141 atcgtactca tcggcgtgct cgactttcac acgcagg
//
LOCUS       DAZFYC010000010        64533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFYC010000010 DAZFYC010000000
VERSION     DAZFYC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.010"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..754
                     /locus_tag="ACHKW5_000810"
     CDS             38..754
                     /locus_tag="ACHKW5_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7510249.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINNQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            784..1284
                     /locus_tag="ACHKW5_000811"
     CDS             784..1284
                     /locus_tag="ACHKW5_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO7510250.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1376..4060
                     /locus_tag="ACHKW5_000812"
     CDS             1376..4060
                     /locus_tag="ACHKW5_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO7510251.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4057..5145
                     /locus_tag="ACHKW5_000813"
     CDS             4057..5145
                     /locus_tag="ACHKW5_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO7510252.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5233..6531)
                     /gene="exuT"
                     /locus_tag="ACHKW5_000814"
     CDS             complement(5233..6531)
                     /gene="exuT"
                     /locus_tag="ACHKW5_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO7510253.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7014..8426
                     /gene="uxaC"
                     /locus_tag="ACHKW5_000815"
     CDS             7014..8426
                     /gene="uxaC"
                     /locus_tag="ACHKW5_000815"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO7510254.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8441..9928
                     /gene="uxaA"
                     /locus_tag="ACHKW5_000816"
     CDS             8441..9928
                     /gene="uxaA"
                     /locus_tag="ACHKW5_000816"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO7510255.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVVFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10011..10562
                     /gene="ygjV"
                     /locus_tag="ACHKW5_000817"
     CDS             10011..10562
                     /gene="ygjV"
                     /locus_tag="ACHKW5_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO7510256.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10567..11811)
                     /gene="sstT"
                     /locus_tag="ACHKW5_000818"
     CDS             complement(10567..11811)
                     /gene="sstT"
                     /locus_tag="ACHKW5_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO7510257.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLNRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12210..13175)
                     /gene="alx"
                     /locus_tag="ACHKW5_000819"
     CDS             complement(12210..13175)
                     /gene="alx"
                     /locus_tag="ACHKW5_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO7510258.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13458..14444)
                     /gene="ygjR"
                     /locus_tag="ACHKW5_000820"
     CDS             complement(13458..14444)
                     /gene="ygjR"
                     /locus_tag="ACHKW5_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7510259.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VGAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14523..15206)
                     /gene="ygjQ"
                     /locus_tag="ACHKW5_000821"
     CDS             complement(14523..15206)
                     /gene="ygjQ"
                     /locus_tag="ACHKW5_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO7510260.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15283..15786)
                     /gene="ygjP"
                     /locus_tag="ACHKW5_000822"
     CDS             complement(15283..15786)
                     /gene="ygjP"
                     /locus_tag="ACHKW5_000822"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO7510261.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15871..17007
                     /gene="rlmG"
                     /locus_tag="ACHKW5_000823"
     CDS             15871..17007
                     /gene="rlmG"
                     /locus_tag="ACHKW5_000823"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO7510262.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17198..17281
                     /locus_tag="ACHKW5_000824"
                     /pseudo
     CDS             17198..17281
                     /locus_tag="ACHKW5_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17278..17694
                     /gene="higA"
                     /locus_tag="ACHKW5_000825"
     CDS             17278..17694
                     /gene="higA"
                     /locus_tag="ACHKW5_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO7510263.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPSGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17739..19757)
                     /gene="fadH"
                     /locus_tag="ACHKW5_000826"
     CDS             complement(17739..19757)
                     /gene="fadH"
                     /locus_tag="ACHKW5_000826"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO7510264.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20083..22434)
                     /gene="ygjK"
                     /locus_tag="ACHKW5_000827"
     CDS             complement(20083..22434)
                     /gene="ygjK"
                     /locus_tag="ACHKW5_000827"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO7510265.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTRKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22451..23521)
                     /gene="ygjJ"
                     /locus_tag="ACHKW5_000828"
     CDS             complement(22451..23521)
                     /gene="ygjJ"
                     /locus_tag="ACHKW5_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO7510266.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23655..25088)
                     /gene="ygjI"
                     /locus_tag="ACHKW5_000829"
     CDS             complement(23655..25088)
                     /gene="ygjI"
                     /locus_tag="ACHKW5_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7510267.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25151..25600)
                     /gene="ebgC"
                     /locus_tag="ACHKW5_000830"
     CDS             complement(25151..25600)
                     /gene="ebgC"
                     /locus_tag="ACHKW5_000830"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO7510268.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25597..28689)
                     /gene="ebgA"
                     /locus_tag="ACHKW5_000831"
     CDS             complement(25597..28689)
                     /gene="ebgA"
                     /locus_tag="ACHKW5_000831"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO7510269.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRGFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28873..29856)
                     /gene="ebgR"
                     /locus_tag="ACHKW5_000832"
     CDS             complement(28873..29856)
                     /gene="ebgR"
                     /locus_tag="ACHKW5_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO7510270.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30075..30407
                     /gene="ygjH"
                     /locus_tag="ACHKW5_000833"
     CDS             30075..30407
                     /gene="ygjH"
                     /locus_tag="ACHKW5_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO7510271.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30449..31828)
                     /gene="ygjG"
                     /locus_tag="ACHKW5_000834"
     CDS             complement(30449..31828)
                     /gene="ygjG"
                     /locus_tag="ACHKW5_000834"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO7510272.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32246..33766
                     /gene="aer"
                     /locus_tag="ACHKW5_000835"
     CDS             32246..33766
                     /gene="aer"
                     /locus_tag="ACHKW5_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO7510273.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33920..34543)
                     /gene="nfeR"
                     /locus_tag="ACHKW5_000836"
                     /gene_synonym="yqjI"
     CDS             complement(33920..34543)
                     /gene="nfeR"
                     /locus_tag="ACHKW5_000836"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO7510274.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34820..35584
                     /gene="nfeF"
                     /locus_tag="ACHKW5_000837"
                     /gene_synonym="yqjH"
     CDS             34820..35584
                     /gene="nfeF"
                     /locus_tag="ACHKW5_000837"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO7510275.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35638..35713)
                     /locus_tag="ACHKW5_000838"
     tRNA            complement(35638..35713)
                     /locus_tag="ACHKW5_000838"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35678..35680),aa:Ile,seq:cat)
     gene            35838..36344
                     /gene="mug"
                     /locus_tag="ACHKW5_000839"
     CDS             35838..36344
                     /gene="mug"
                     /locus_tag="ACHKW5_000839"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO7510276.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36423..38264)
                     /gene="rpoD"
                     /locus_tag="ACHKW5_000840"
     CDS             complement(36423..38264)
                     /gene="rpoD"
                     /locus_tag="ACHKW5_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO7510277.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38459..40204)
                     /gene="dnaG"
                     /locus_tag="ACHKW5_000841"
     CDS             complement(38459..40204)
                     /gene="dnaG"
                     /locus_tag="ACHKW5_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO7510278.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40315..40530)
                     /gene="rpsU"
                     /locus_tag="ACHKW5_000842"
     CDS             complement(40315..40530)
                     /gene="rpsU"
                     /locus_tag="ACHKW5_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO7510279.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40768..41781
                     /gene="tsaD"
                     /locus_tag="ACHKW5_000843"
     CDS             40768..41781
                     /gene="tsaD"
                     /locus_tag="ACHKW5_000843"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO7510280.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41824..43287)
                     /gene="ttdT"
                     /locus_tag="ACHKW5_000844"
     CDS             complement(41824..43287)
                     /gene="ttdT"
                     /locus_tag="ACHKW5_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO7510281.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43336..43941)
                     /gene="ttdB"
                     /locus_tag="ACHKW5_000845"
     CDS             complement(43336..43941)
                     /gene="ttdB"
                     /locus_tag="ACHKW5_000845"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO7510282.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43938..44849)
                     /gene="ttdA"
                     /locus_tag="ACHKW5_000846"
     CDS             complement(43938..44849)
                     /gene="ttdA"
                     /locus_tag="ACHKW5_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO7510283.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPILVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45056..45988
                     /gene="ttdR"
                     /locus_tag="ACHKW5_000847"
                     /gene_synonym="dan"
     CDS             45056..45988
                     /gene="ttdR"
                     /locus_tag="ACHKW5_000847"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO7510284.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(46001..46618)
                     /gene="plsY"
                     /locus_tag="ACHKW5_000848"
     CDS             complement(46001..46618)
                     /gene="plsY"
                     /locus_tag="ACHKW5_000848"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO7510285.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46723..47091
                     /gene="folB"
                     /locus_tag="ACHKW5_000849"
     CDS             46723..47091
                     /gene="folB"
                     /locus_tag="ACHKW5_000849"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO7510286.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47182..48003
                     /gene="bacA"
                     /locus_tag="ACHKW5_000850"
     CDS             47182..48003
                     /gene="bacA"
                     /locus_tag="ACHKW5_000850"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7510287.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48166..49404)
                     /gene="cca"
                     /locus_tag="ACHKW5_000851"
     CDS             complement(48166..49404)
                     /gene="cca"
                     /locus_tag="ACHKW5_000851"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO7510288.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(49468..50088)
                     /gene="ygiM"
                     /locus_tag="ACHKW5_000852"
     CDS             complement(49468..50088)
                     /gene="ygiM"
                     /locus_tag="ACHKW5_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO7510289.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            50330..51631
                     /gene="ygiF"
                     /locus_tag="ACHKW5_000853"
     CDS             50330..51631
                     /gene="ygiF"
                     /locus_tag="ACHKW5_000853"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO7510290.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51654..54494
                     /gene="glnE"
                     /locus_tag="ACHKW5_000854"
     CDS             51654..54494
                     /gene="glnE"
                     /locus_tag="ACHKW5_000854"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO7510291.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            54542..55975
                     /gene="hldE"
                     /locus_tag="ACHKW5_000855"
     CDS             54542..55975
                     /gene="hldE"
                     /locus_tag="ACHKW5_000855"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO7510292.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56095..56154)
                     /gene="ibsE"
                     /locus_tag="ACHKW5_000856"
     CDS             complement(56095..56154)
                     /gene="ibsE"
                     /locus_tag="ACHKW5_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO7510293.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56471..56530)
                     /locus_tag="ACHKW5_000857"
     CDS             complement(56471..56530)
                     /locus_tag="ACHKW5_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7510294.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(56769..58430)
                     /gene="yqiK"
                     /locus_tag="ACHKW5_000858"
     CDS             complement(56769..58430)
                     /gene="yqiK"
                     /locus_tag="ACHKW5_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7510295.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(58457..59086)
                     /gene="yqiJ"
                     /locus_tag="ACHKW5_000859"
     CDS             complement(58457..59086)
                     /gene="yqiJ"
                     /locus_tag="ACHKW5_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7510296.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            59355..59555
                     /gene="glgS"
                     /locus_tag="ACHKW5_000860"
     CDS             59355..59555
                     /gene="glgS"
                     /locus_tag="ACHKW5_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO7510297.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(59598..60662)
                     /gene="yqiI"
                     /locus_tag="ACHKW5_000861"
     CDS             complement(59598..60662)
                     /gene="yqiI"
                     /locus_tag="ACHKW5_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510298.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(60664..61413)
                     /gene="yqiH"
                     /locus_tag="ACHKW5_000862"
     CDS             complement(60664..61413)
                     /gene="yqiH"
                     /locus_tag="ACHKW5_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7510299.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            complement(61429..63933)
                     /locus_tag="ACHKW5_000863"
     CDS             complement(61429..63933)
                     /locus_tag="ACHKW5_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7510300.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            complement(63993..>64533)
                     /gene="ygiL"
                     /locus_tag="ACHKW5_000864"
     CDS             complement(63993..>64533)
                     /gene="ygiL"
                     /locus_tag="ACHKW5_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO7510301.1"
                     /translation="KKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDE
                     DLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQT
                     DLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPS
                     KDATPGNVYAQADYKIAYE"
BASE COUNT        15708 a        16151 c        16874 g        15800 t
ORIGIN      
        1 caactttttg gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt
       61 atcacggctt acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac
      121 tgtttatcct atggctgttt ccattaataa tcagggtgaa ggcaacgttc gcgtaatatc
      181 taaaagtaat gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac
      241 gcctcaggaa aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc
      301 acctaaattc gctttaccag ccggtagtag caaaaccgta cgttttgttg cgatggaacc
      361 agagcaaaaa gagaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt
      421 tgccacagat aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt
      481 tgttagtgtt ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt
      541 agttaatgca gggaatcaac gcttaaagat tttaacgatc gcttattgta aaaataatag
      601 caaagaaaat tgtaagatac agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa
      661 tcttgaaagc atatctggct acgacaaaat cgttgtcaaa tataataact ggatcaccaa
      721 agataatggc gagtttgaac tggcggtcca ttaattatca ttgtaataaa cgaaggaata
      781 gtaatgaaaa aggtatttgc aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc
      841 tccgcattgg cagtacaaaa ggatattact gtaacggcca acgttgatgc cgctctggat
      901 atgacacaga ccgataacac cgcgttgccg aaagcagtag aaatgcaata tctgccgggg
      961 cagggtctgc aatcttacca gttgatgacc aaaatctggt ctaacgacgt taccaaagat
     1021 gtaaaaatgc agttagtttc cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt
     1081 gttccgctga cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc aaccactttt
     1141 accgcaacca aaatctttgc ttccgatgcg ttaactaacg gttctctggc taaaaacctg
     1201 atgtttgctc aaaccaccaa aggtgttctg gagacaggca tctaccgtgg tgtagtaagc
     1261 atttatctgt ctcaggatat ctaatccaga taacagaatt aacacgtaac aaagggaggg
     1321 gtagccctcc cttattttaa ctgttttagt atttgttatg gatttcagga ttgccatgga
     1381 taaaaaatta ctggctcttt tgatcctggc gagtctcagc ccggcagagg cgacattaac
     1441 caaaattccc gcagggtttg aggttattgc tcagggacag caggagtata tcgaggttta
     1501 tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt aatcttgata ccgtaacatt
     1561 tcttgatcca gcaagtctat ataacaagct ggaactggat gtagacgatc agaaaatcgc
     1621 gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg
     1681 ttatgtacgt actgactcag ggtgtggttt tctgaatacc gatacgctgg aaataatcta
     1741 taatgatgaa gaaagttcgg caacgttgtt tattaatccg caatggaatt cagctttcga
     1801 tgcgaagtca ttatatttaa atccagacaa aaatactgtt aatgctttta tacatcagca
     1861 agacatcaat gttctggcac aggatgatta ccaatcgttg tctattcagg gaaacggtgc
     1921 gctgggaata acagaaaata gttatattgg tgcacactgg aatttcaacg gttatgatgc
     1981 agatgatgtc agtgacagta atgctgacgt cagcgatctc tattatcgtt atgatttttt
     2041 acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc acactattta atgcacaagg
     2101 cgggaacttt acctttaact ttctgccact cggtgcaatt gatgggatgc gtatcgggtc
     2161 gacactgagc tatttaaacc aggcgcaaag ccagcaagga accccagtaa tggttctgct
     2221 ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa cttttgggat cgttttatct
     2281 caatagtggt tcgcaattta ttgataccag ttcctttccg ccaggcagct acagcgtggc
     2341 attaaaagtc tacgaaaata accaactcac ccgcaccgag cttgtgccgt ttaccaaaac
     2401 cggcggtctg actgacggaa atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca
     2461 ggtttctgat gatgaaagtt cagcttatca gctaggagta cgcctgccat tacatccgca
     2521 atatgagctc tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa
     2581 taactggacg gcagatttag gcggggtggg gaatcttgca atcagcgcca gcgtgttccg
     2641 taacgatgac ggcggcaaag gtgatatgca acaggccaac tggagtaatc cgggatggcc
     2701 gacgttgggc ttttatcgga ccaactctga cggcgatgct tgtacaaccg acagcagaga
     2761 gagctataac gccttaagct gttatgaaag tatttccgcg acggtttcac agaattttgt
     2821 cggctggaat atgatgctgg gttatacccg cacacaaaat aacactgatg atagtttgcg
     2881 ttgggataaa cagcagagct ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat
     2941 ctctgaaact gtacaactta gcgcttcccg cgcttttgtg atgcgtgact ggattttgag
     3001 tacgtcagtc ggtgttttcc atcgtaatga caacggtggc gataacgacg acaacggctt
     3061 gtacttatcg ttttcgttat ctgacacgcc aacgatggat agcaataaca acagccattc
     3121 aacaaatgtg tctacggatt atcgttatag cgaacaggat ggcgatcaaa cgtcatggca
     3181 gttatcgcat accttttata acgattcatt cagccataaa gaacttggcg taaccgtcgg
     3241 aggcctgaac accgatacca taaacagcgc ggttaacggg cgttgggatg ggcaatacgg
     3301 aaatgtctac gctaccgtat ctgatagtta tgaccgtaag aatcatgatc atctttcggc
     3361 ctttacgggc acttatagct ctacgctggc tgtcagtcgc tatggcgtta atttgggagc
     3421 cagtggtaca gacgatttgc tgggtgcggt actggtggat gtgaaaggct tctctgaaca
     3481 ggatgaagat agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc gaactttgca
     3541 gcttggtcaa agtgacagtg tgttgttccc ttatcctgga tttcagtctg gttttgttga
     3601 ggttaacgac agtagccagg gcaatcagca ggggacaaca aacatcatta acggtgcagg
     3661 gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt
     3721 taactacaac tatatcggtc gtttattact gccggcagcg gtgaaaaaat tcccgattgt
     3781 ggggctgaac agcgccatgt tactggtagc tgaagatggc ggatttacac ttgaaattaa
     3841 cggcagcgaa aaagagctct atctgctttc cgggcagcaa ttccttaagt gtccactgag
     3901 tgttgtaaag aaacgcgcca gcattcgtta cagcggagat gttacttgta gtgtggtgac
     3961 ttattcacaa ttaccggagt cgattcaggt tcaggcacag ttgaaacagc ctaaattacg
     4021 tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt gattgcggcg
     4081 atattgggct tgtttggcac gctcactggc gttcaggcag ctcctgacgt gacaagtgaa
     4141 attacgtatg atttggcatc tggtagagcg gattattact tctggaagga tgaagcgtcg
     4201 gcaggaaata atggatatat gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt
     4261 acagctaatg gaaatatatc gacagtacaa atctatttaa ctgaacaacg cagtgggatg
     4321 cgttggccgg taaaactcaa aggatttaaa acagccattg taagtagcga tgaagcgccg
     4381 ccaggatgca aggggggcaa agggcttcag acgaatctta aggattctaa tagatcttca
     4441 tgtacagaag atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt
     4501 gagcagacag agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta
     4561 cattcgaaca gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc
     4621 aaccatattg acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac
     4681 ttgcatccaa caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac
     4741 atgtgcctgt acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat
     4801 gaagggcgac cagctgcagg gcgcagagac ggttacttct ctatctatcg tcagggaggg
     4861 accaccaccg acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc
     4921 ggtgggcaaa ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa
     4981 cgtgtgcgtc cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc
     5041 ttgactctgg cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc
     5101 aaattgtcag taatctttac gccttccttg ccaaccatca attaaccctg caaaccggac
     5161 aaacgctttt ttctatataa acgacgaagc cgtctgataa ggcggcttcg tcattacaac
     5221 ttaagttaaa cgttaatgtt gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat
     5281 cgccggtttg ttctgcaaca cggtccagat aaccagcgca cctaacaggt caaatactgc
     5341 cagaactgcg aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc
     5401 aaacagcgta cttgccagcc atgcggacat cccggttaaa ccgttcgccg tcgccacttc
     5461 gttacgaccg aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc
     5521 aaaaccaccg atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat
     5581 acccgggcca atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag
     5641 gttcacacca aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag
     5701 gtcagcaaac agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta
     5761 aactttaaac atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgccag
     5821 gaaacgcggc agcgcgatac cccagaactg acggttacgc aggatctgac caacggacat
     5881 tttcttcgcc gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc
     5941 ttcatcggtc agatgcttct ggtcgcgtgg atgtttatag aaaatcagcc atgccatcgc
     6001 ccagataaag ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcattacgat
     6061 tgcccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     6121 acctaccgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     6181 cgggatcatc gctgcttccg cggcaccgac tgcaccacga gcaatcgcca ggccacccca
     6241 gctaccagcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     6301 accgattttc gtacccagca catccagtac atagcccgct accggttgca tgaccgtata
     6361 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     6421 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     6481 aagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     6541 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     6601 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     6661 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     6721 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt
     6781 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     6841 aggccagtac tggcgcggcc tgcagagaga tttaccactt tgagagtaat ttttttaact
     6901 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     6961 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     7021 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     7081 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     7141 aagactatcg ttttaaaaac ctgtatgaca tctggctgaa aggcgatcac tacaaatggc
     7201 gcgctatgcg taccaacggt gtggccgagc gtctgtgtac cggtgatgcg tctgaccgtg
     7261 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     7321 ggacgcacct cgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa
     7381 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     7441 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     7501 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     7561 gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac ctttaacgac tacatggcga
     7621 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     7681 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     7741 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     7801 ctggcgaacc cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     7861 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     7921 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     7981 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     8041 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     8101 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     8161 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     8221 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     8281 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     8341 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     8401 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     8461 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     8521 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     8581 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     8641 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     8701 ggatcagtat cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga
     8761 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaatgagc tgtggatcct
     8821 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     8881 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc
     8941 acaactgggc gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc
     9001 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     9061 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     9121 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     9181 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     9241 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     9301 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     9361 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     9421 cttcaaacag tactttattg cccatgacca gccgatctat gaaaacccat cgccggggaa
     9481 caaagcgggc ggtatcacca cgctggaaga taaatccctt ggctgtaccc agaaagcggg
     9541 ttccagcgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cggggctgaa
     9601 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     9661 ccatatggtg ctgttcagta ctggtcgtgg cacgccatac ggcggctttg taccgacggt
     9721 gaaaatcgct accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     9781 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     9841 cgttgtgttt gccaacggta aacaaacctg caacgagcgt aacgacttcc gcgaactggc
     9901 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     9961 ttcataatgt gaagcgccgt ttgcttttcc atacctgata aggatttatc atgaccgcgt
    10021 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
    10081 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
    10141 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
    10201 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
    10261 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
    10321 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
    10381 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacaatga
    10441 ttgagggtag ttttctgctt atgaatggct tgaatatcat tcgtttctgg cggatgcaga
    10501 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccgtagac gaaaggggtt
    10561 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
    10621 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
    10681 gccgatgata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
    10741 attacaggcc agcgggatca gcagcagaga accccccgcc acgccggatg cgccacaggc
    10801 acacagagaa gccaccacgc tcaacagcag cgccgtgggc agatcgaccg ggatacccag
    10861 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgccag ccatattgat
    10921 ggtggcaccc agcggaatag aaacggaata ggtatcgcga ttcagattca gcttttcaca
    10981 cagtgccata ttcaccggaa tgttcgccgc agagctgcgg gtgaagaagg catacacgcc
    11041 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
    11101 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
    11161 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagacc
    11221 aaaaatacca atcggtgcga agcgaatgac cagtttcacc ataaaggtga cggcattcga
    11281 catatcgttg accaggtttt tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc
    11341 aattgcccac accaggatcc cgatgtagtt acctttcagc agcgcgtcga tggggttgga
    11401 aaccatgctc attaccagcc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc
    11461 acccgcgcta ctggataaat gcagggtaga agggaaggca aagctgaaga ctactgcggc
    11521 cagagcagca gagaaggtac ccagcagata gaggaacaaa atagggcgga tattggtttt
    11581 ctgcccgtgc tggtggttag caatagatgc catcaccagc atcaacacca ggatgggggc
    11641 aacggctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
    11701 ttttgagatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    11761 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    11821 ctttcattgt gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    11881 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    11941 gattttaaag agaggtcata ttttgcaact taatggttac atttatttga cataaaaata
    12001 aagccaacgt gcggcttcca gactgatgac aacatcaaaa aaggactcgg tcagtcccct
    12061 cgccccgttt ggggagaggg ttagggtgag gggaacaggc cagcatcggt gcgaaaaatt
    12121 accctcaccc cgaccctctc cctggaaggg agagggagaa aacggtgctc atgttaatga
    12181 cagggtacac ggcttaggca gattaaaaat tatccacccc gctgcttatc atgccgataa
    12241 ttcacccagg cgttgataat aaacgtcatc accagaatgc caaacaccac gcccagcgag
    12301 acggcgattg gaatatggta gaagtcgaca atcagcatct tgataccgat aaacaccaga
    12361 atcaccgcca ggccatattt gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa
    12421 tacatcgcac gcaggccgag gatcgcaaac aggtttgagg tcagcacaat gaacgggtca
    12481 gtggtcacgg cgaagatagc cggaatgcta tccacggcga aaatcacgtc gctcaattcc
    12541 accagaatca gtaccagcat cagcggtgtg gcgtacagca atccattctt acgcacaaag
    12601 aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc
    12661 ggcttgtctc caatgcctga ttcatcttca tgggcgagcg ccatcttcac gccggtaaac
    12721 agcaggaagg caccgaagat atacaggatc cagtcgaact gcgaaattag ccagctgcca
    12781 gtgaagatca tgatggtacg cagaacaatc gccccgagca cgccatacac cagcacgcgg
    12841 cgttgtaatg ccgccggaac agagaaatag ctgaacaaca tcagccagac aaagacgtta
    12901 tcgaccgcca gcgatttctc aatcagataa cctgtgagaa aggccagtgc ctgtggatcg
    12961 gcaacggcgc gaccttcggt ttgcaccaga taccaccaga aagcggcgtt aaacagtaac
    13021 gacagcgtca cccagaccag cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc
    13081 ccacgacgcc cctgcaacaa caggtcgata gccagcataa tggcgacaac aacagcgaat
    13141 ccgccccata gcaacggcgt gccgacagta ttcatagaag ttccttacac ataaaaaaac
    13201 ggccaacgtc ggaagacgtc agccgctgct ttttatgcat agacctcgcc ttccggcaag
    13261 gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc
    13321 ggatgtggtt tttcacacat cgtaatgacg atcgaccggc aatgaagtta ctcccctttg
    13381 caggtaacaa agtatgacag accattcagc cagtcaatgg tctgttacat accttttaca
    13441 ctgttttact ttggcaatta tagttttacg ctatctgccg gaaaaatcac cccagtctgg
    13501 cggcggatct cggtcagcag tttggcggtg atgcgactga ccgccagccc cggatgatcc
    13561 accagatgct catccaccag ggtagcgaac agctctgctt cgtagagcat ggtattaata
    13621 tgctgcggct gggtgagatc ctgcatttgg ctgccacgcg gcacgaagca cactttctgg
    13681 cattcagaca gtttttcaat caccagcgat cctgcttcgc cctgaatctc gctcgccagg
    13741 acagaatcac tgacttttga gtgctgcaag gtgacgctga aatcaccgta atccatcacc
    13801 accacgccgt gggcatctac accgcttgcc agcaaactgg cggttgcctg cacgcttttc
    13861 ggctcaccaa ataacgccac cgccgacgcc aggcagtaaa agccgatatc cataattgaa
    13921 ccgttagaga atgacggatt aaaggtgttg ggattttcac cgtccaggta acgttgatag
    13981 cgcgaggaat actggcaata gttgaaaaag actttacgca atttgccgac tttcggcagc
    14041 gcctggcgca gcaaatgaaa gttcggcagg caggcggttt taaatgcctc aaacagcacc
    14101 acctgatttt cccgcgcaca ggcaatggcg gcacccactt ccgccagatt cgacgccagt
    14161 ggtttctcgc aaatcacatg aattttatgg ctaaggaaaa gttgtgtctg ggaaaaatgc
    14221 agggcattcg ggctggcaat atacaccgcg tcaatggcat cgctttccgc catcgcttcc
    14281 agcgaggtaa acagatgctc gacagaaaaa tcattggcga agtgctgagc ctgctcaagg
    14341 ctgcgggaat atacggcggt taacttgtat ttaccgctct catgggcggc ctcgacgaac
    14401 tggcgagtga tccagttcgt accaatcaca gcgaaacgta tcataacgcg tggatactcc
    14461 ataaagggat tctgtcgtca atgtagcacg cctttttcat cttcgacgtg cgcagatacg
    14521 cattattctt ttgccggaca gccatgctca ctaaacggcc caatcatgac cgatggcccg
    14581 agatatttcg gctggcgatg gaggattttg gcgtcaatca cggcgctgac acgagcgagt
    14641 ttctcccgca gttgagtata aaaaccgtgc ttcatattga ggtcaggcgc gttaaaaccg
    14701 atagcgtcga tgctgtactg ttttgccagc cagatggcgc gttggttatg aaattcttgc
    14761 gagataatgg tgatatggtt ttcaccaaaa acctttttgg cacgcactac cgaatccagc
    14821 gttgagaatc cggcatagtc gcagaagatc actttcgccg gtacgccttt agcgatcaaa
    14881 gcctgctgca tacctgaggc ttcatcgtaa tttttacgtc cgttatcgcc acttaccagt
    14941 agccatttca cttttccggc gtggtacaac gctgctgcgg tatcaatacg tcgggtaaag
    15001 tagcgattac ccggcctggc ccccagcaat aagcccacgt tacgctccgg aactgcgttg
    15061 acatcgctcc aggtcagctg cttgctggcg ttgaccataa cgcgatcggc aataaaaatg
    15121 gtcgctcctg ctataagcaa caacaaacag ccaatcttaa gagtccggcg agaaaaacaa
    15181 atacggagaa gaaggcgggg ctgcatggtc gttatcctta acgaaagggg ggaggtcgat
    15241 tttaggcgct aaagaaagaa aagataacgc ctgacatcat tttcagattt tgtcctgccc
    15301 aagcgacaac tgcgttagcc acagccgggt gtcgaactca agctggtggt actgcggctc
    15361 catatggcag cacaactggt aaaacgcttt gttatgctct ttctccttca ggtgcgccag
    15421 ttcgtgcacc acgatcatac gtaaaaacgg ttccggcgcg ttgcgaaaca cagtagcgac
    15481 gcggatctct gcttttgctt tcagcttgcc gccctgcaca cgagaaacag cggtatgtaa
    15541 cccgagcgcg ttttttagca catggatctt attgtcatac atcactttat tgatcggcgg
    15601 ggcattacgc agaaactgat ttttcagatc ctgagtatat tgccagaggg ctttatcggt
    15661 ggcgtagtcg tgtgttcccg gatagcgttt tgccagcaca tcgcccagac gctgttcgtt
    15721 aatcagcgtt cgcacctggg aaagtagctg ctcgggataa ccctgaagat aagtaagatt
    15781 gttcataacc gcccacgaaa aagagaaaaa gggtatactc acgcaccctt ttcaggggca
    15841 acgtcgaaat tttatcattc aggagggccg atgagccact tagacaacgg tttccgttca
    15901 ctgacactac aacgttttcc ggcgacggat gacgttaacc cgctacaggc gtgggaagcg
    15961 gcggatgaat atttgctgca acagttggac gacacagaaa tccgcggccc ggtgttgatc
    16021 ctgaatgatg cctttggtgc gttaagctgc gcgctggcgg aacataagcc gtacagcatt
    16081 ggcgactcat acatcagtga actggcgacg cgcgagaatt tacgcctcaa cgggattgat
    16141 gagtcgagcg tgaagtttct cgacagcacc gccgactacc cgcaacagcc gggtgtggtg
    16201 ctgatcaaag tgccgaaaac actggcattg ctggaacagc aactgcgtgc gctgcgcaaa
    16261 gtggtcacgt cggatacacg tattattgcc ggtgccaaag cccgtgacat tcacacttcc
    16321 acgctggagc tgttcgagaa agtgctcggc ccgaccacca ctacgctggc atggaaaaaa
    16381 gcgcgcctga ttaattgcac tttcaatgaa ccgccgctgg ccgatgcgcc gcagaccgtt
    16441 agctggaagc tggaaggtac tgactggact atccacaacc atgcgaatgt cttctcccgc
    16501 accgggctgg atattggcgc acgcttcttt atgcaacatc tgccagagaa tctcgaaggt
    16561 gagattgtcg atctcggctg tggtaacggc gttattggcc tgacgctgct tgataaaaac
    16621 ccgcaggcga aagtggtgtt tgtcgatgaa tcgccgatgg cggtcgcttc cagccgaatg
    16681 aatgttgaaa ccaacatgcc agaggcgttg gatcgctgcg agtttatgat caacaacgcg
    16741 ctctccggag tggagccttt ccgctttaat gctgtgctct gcaacccgcc gtttcatcag
    16801 caacatgcgc tgaccgataa cgtcgcctgg gagatgttcc accacgcccg ccgctgcctg
    16861 aaaatcaacg gcgaactgta tatcgttgcc aaccgtcacc tggattactt ccataaactg
    16921 aagaagattt tcggcaactg caccaccatc gccacgaata ataaatttgt ggtgctgaaa
    16981 acagtgaagc tggggcgtcg tcggtaagat tgggagttac ctgccggatg cggcgttaac
    17041 gccttatccg gcctacgttc agcacgttac tcccaggccg gataagacgc ggcaagcgtc
    17101 gcatccggca atgtctgccc agttctggaa tcgcgcttcc ataaaacaaa aaatccctgt
    17161 caccatcctc tacattctct tgtttagcgt tgttcaacgt ttatgctaca tacgtgaagt
    17221 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    17281 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgcggtggc accttttctg
    17341 gcgggtattc agaacgagga acaatacgcc caggcgctgg aactggtaga tcatctgctg
    17401 ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    17461 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tagcggtata
    17521 gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt
    17581 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    17641 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    17701 gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    17761 gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt
    17821 aaatgcacag ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    17881 cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca
    17941 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    18001 gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg
    18061 ctggctttgc gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc
    18121 gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg
    18181 gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    18241 atcccgccac aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca
    18301 tcgagataac tcaatacctt cggatgatcg atcccgtcga taggcggaac gcgcggcacg
    18361 atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga
    18421 ttgagtttca gcgtcacgcc cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg
    18481 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg
    18541 tgggcatcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    18601 ccagcaggtc ccgcaccgac caccgccaga ttttttttct gcacggcagg aaggattggc
    18661 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    18721 atctgatcga ggcaggcctg attgcagcca atgcaggtgt tgatctcatc ggctcgcccc
    18781 gattgcgctt ttgacagcag ttccgcatca gcaagaaacg gtcgtgccat cgacaccata
    18841 tcggcatcgc cgcgcgagag aatgtcgtcg gcaacctgcg gatcgttaat ccggttggtg
    18901 gtcaccagcg gcagcgaaac gtggcctttc agtttgcgcg tgacccaagt aaatgcgccg
    18961 cgcggcacgg gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg
    19021 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc
    19081 ccgccgtctt cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg
    19141 acgcgttcgc gcaccgcacg cactacttct acggcaaatc gcatccggtt gcggtaatcg
    19201 ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac
    19261 ccttcggacc ccattacctc tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag
    19321 tgggcgaaat cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa
    19381 cggttgatgg gggcctgcaa tgcggaggga gcgaccagat gcggttggta gctgtagcgc
    19441 ccggtatgca aaatttgcag ggctattttg ccgccttcct gatgtaccgc ttcggtaatg
    19501 gtgcgatggt gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg
    19561 cctgttaaat ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt
    19621 tcggcataaa acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc
    19681 attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    19741 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga
    19801 gttctaattt agccgtcgcc ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga
    19861 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgcgt tttatccggc
    19921 ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcacgg
    19981 tgcagatgcc ggatgcgacg cttgtcgcgt tttatccggc caggaggatt gcacatgtag
    20041 ggcggataag gcgtttacgc cgcatccgcc agcagttgca aattattgct tacggaaaaa
    20101 atcgttatac aacatataca aatgcgcggc actccaggag aaatttggtg cgccttgctg
    20161 tgcgccagtc agcgggttgt aattctcctg aattgggcca tcggcggtta atcctttggc
    20221 gtgacggaag aacgtatccg ccagtttcag ggcatcatcg cgataaccgt aacgctccat
    20281 ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccacgccagt agatatcagc
    20341 gccaaaagcc ggattggtta acgccgccgt tcccagcggg acaaaggtgt tgaactcttt
    20401 aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa
    20461 cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc
    20521 gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag tcgggtcgaa
    20581 catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc
    20641 ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc tgtacatata
    20701 gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc cgtcctgact
    20761 gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag cgacatattt
    20821 atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacgcc ccgattccca
    20881 cgacgcagcg acctgtgccg gaatttccag actgtcgtac tggcctttct ccaccacgcg
    20941 ggcgtagttg ttcagcccag actgcgtctc ttctttgttg cctttcttca ccgtaaacag
    21001 catctcgccg ctctcagtgt tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc
    21061 gttgccgttg tgatcgcggt tacgtaacca ccagtcgtga taggccacca gtttcgggta
    21121 catctctgcc aaccaggctt tatcctgggt gacgttgtac acttccatca ccgaccaggc
    21181 ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac cacgctcggg
    21241 gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac gcacgctatc
    21301 gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga tgtcgggatt
    21361 gaaatgcgcc atcgcaaacg cctgcttcct ggtatcccac ggccaggtct gattgccgga
    21421 gaaccagcgc ccggtcaccg acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga
    21481 gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt
    21541 cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct gcgaggcggt
    21601 gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt tgctaacttc
    21661 ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc
    21721 cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt tatgcacctg
    21781 atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc caaaggtgac
    21841 tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt cgccagcaat
    21901 ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca gttcgccgtc
    21961 ccacaccaga tccagcggtt tattgctggt gattttggtt tccagtagtg acgtgcgcgg
    22021 cgtggcgaag cgcagagtca tttcgacttg cacatctttt gctgtcagtt tttgcaccag
    22081 cgcaccggga atactgtatg cctccagcgt aaagtcgact ttcttgccgt cctgccagac
    22141 ggtcaggcgg tcgaaattgc tggccataaa gttgatgtac tcttccgtca gcagcgcaac
    22201 gcccggaaag ccgcccatgg tgttagggcc gtctggcaac agatgaccat gccaggctcc
    22261 gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg
    22321 cggcgcgcca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg catgggcgga
    22381 aaaacttatc agcagagcgc aggttactgg cgttaaaata gtttttattt tcatgaaatt
    22441 acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg ggatcgtgag
    22501 aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt
    22561 cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat
    22621 gtttggcgta ggtgtaagcg gtggagagat aaacgccctt taacgcttca tacatggcgc
    22681 tcaccatcat cccactttca cttcccggta cgtccagccc gtcggcgcgt ccggtatggt
    22741 gccaggcgcg agcggcaaag ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc
    22801 cgtcggtgat ttccagaccg ttcatccagg tgatgccttc ggcaaggtca tactggatgt
    22861 aaccgttcat cattgccgga taggtgtatt tgtcgtcata gcgatcgtac ttgccaaaat
    22921 gtagccaggc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg ctataacgaa
    22981 agttgccgct taagttctgc accttcagcg cggtcataat gtcgcgggta ttgggaatga
    23041 cgtagcccag atcgggggtg aagtcgcccc accattgcag gtcatccagc gaggaatcct
    23101 gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca tagaaacggt
    23161 taattccgcc ctcgaaacca ccccagctat ggtctggcac ccaggcattg ccgtcgtggt
    23221 cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct
    23281 ccaggccacc ttcaatatag gtaccgtcgc tgtatttatg tttattctca ccctctaaat
    23341 ccatatatcc accaacccct aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg
    23401 taattgcagg tgtttttgtg tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag
    23461 aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg attaatttca
    23521 ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa ttataaaaat
    23581 ataaaggcag aaactatttt ccccctcccc atccaggaag agggaaaatg cctgctcatt
    23641 ttttattagt ctgctcaatc aacattgctg gctggcgtcg cttcaatatg gcgctcttca
    23701 gccgttaatc cctttatata tttactgtat ttccaccacg caaagccgag gaagataaca
    23761 ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa
    23821 gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat accggtgcgg
    23881 cgggagccca tgcggaaatc gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa
    23941 gccagcatga tgaataacgg cggaagcatg gacgctgcgg cagtcatatt aataatagta
    24001 ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag cgggatgacg
    24061 atcaggaact ggatccacgc tgcgcgcgct ggaacgccgt tttcgttcag ttcgacggtt
    24121 ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc
    24181 cacatcagca gggaaccgaa catcgcggta aaggacacca gaccgacaaa gcgattcatc
    24241 aacgcttccg gtagaccaaa atacgccgcc atgccgtgga atacctgcac cgatccgccg
    24301 gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga gacggaatac
    24361 agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt cgaaccgcct
    24421 ttgacgtcgt taacgtacac agcgacggac tccgcgccac ctgcggccat aaagatccag
    24481 gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac ggtgatgggg
    24541 tcagcaggct gtacgccgcc aaccagcgcc gtacctgcca gtaaaatgta ggagagcgtt
    24601 aacagcagca tcagcgttga agtgacggag gtaattggcc ccaacatttt cgccccgttg
    24661 gtggaaaccc aggtggagaa ggcgaacagc accatactga taatggtggt ggcaaccggc
    24721 gtcataatat attcgtagcc gaggaaggca tacgaagcat aagcaataac gcgcggcaac
    24781 agtgaggtga aaaagaacag gtttacgaac cagtaggtat aggcagtaat aaatgcccaa
    24841 cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt tttatttaac
    24901 gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa
    24961 aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt gttaaagctg
    25021 taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt acgtttggta
    25081 tcagacataa cacatctccg aaaatgtgta gggtagcggt ggcggcaaac tccttttgcc
    25141 gccgtagttg ttatttgtta tggaaataac catcttcgat ggtgactttg agaaccactt
    25201 ttttgaccgc gttattgcag ataaaccgat acgcttcatg gatatcgcaa ataacgattt
    25261 gcccttcgtg gacctcaacg gtttctccgc agccttttaa atattcacgg tcagtttcat
    25321 cacgataata ttccactacc tgtaatgtct ctttcggcgc atattcaatt ttttgctgcc
    25381 cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg
    25441 cggagtctgt ctccacgcgg taagtcaatg agtcaccgat ggagtgggcg acgccaggct
    25501 gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttcttg ccagaggcgt
    25561 aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct cgctgtgcaa
    25621 attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg cggtagcttc
    25681 tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc agacgcgcca
    25741 ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagcagct ggtggtcgag
    25801 gttcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt
    25861 ttcctgggta tagcgccagg cgctgaagtt aattgggcgc tgcggaacca ccagcagacc
    25921 gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc cgttgttctg
    25981 cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat cgatgatgtt
    26041 agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg ccacctgatc
    26101 gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc
    26161 gtaacgctcg ccggaaagcg ccacgttaac ctggccatcg gcagcgatgc gccagatgta
    26221 ggtgcagcgc atcccgaagt caaacaccgg cggggcaata actgtgcggc tgatgatcaa
    26281 cacttcacca tcgctctgtt ctacggcaaa gccgcgcaga tgttcctgca tgatctgcaa
    26341 atgattcggt tgccacagcc cttcgtactc ctgcttgtgg ttgtcgatca ttggcttgaa
    26401 gaagttgatc tttggctcgc gggtcagcag cgactcgcca ttcacctgcc aggatgtcgg
    26461 tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa
    26521 acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca ctggttgcgc
    26581 ggtgttttcc ttcagcggga actgataagt ggcgatagaa tgcccggctt cgctgtagcg
    26641 ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg
    26701 cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt taatctgctg
    26761 cgtcgcgagc gtttcacctt cgacgcgcac ctctgcgtgc agggtgtagt catcaagcgt
    26821 ggtaaaccac agtttatttt cgactttcag ctcgccgtga gtcagatcca gcgcgtggat
    26881 ttttaccggc gcgataacct gtttgtactc tttcaggccc ggtcccggcg tctgatcgga
    26941 atagatcaaa ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc
    27001 gaatttatac cagacgttgc cgttgtcatc ctgcgcctgg atcccgtggt cgcaccattc
    27061 ccagacataa tgtccctgaa tgcaatcgtg cttatagaag acgttctggt actccgtcag
    27121 cccgcctggt ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg
    27181 cgggtattca ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc
    27241 gaccacttca gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcctt
    27301 cgctgcgtgg tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca
    27361 gatgatgatc gacggatggt ttttctgcgc gtgaatatgg cgaacaatgc gctcgacgta
    27421 gactttttcc cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc
    27481 gtgcgattcg acgtcggttt ccgccatcac aaacaggccg tagatatcac acagttcgta
    27541 aaaacgcgga tcgttcgggt agtgagcggt acgcacggag ttgatattgt gctgcttcat
    27601 caactggaga tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt
    27661 gtcgtgacgg ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc
    27721 gcgcactttg atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtt
    27781 ggcgtctttc agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg
    27841 cggctgttcg acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc
    27901 aatggcgctg ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac
    27961 gacaggggag gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata
    28021 ggcttcgtca aagtcggtac gcacagtgaa atcgttaata tgcgttaggt gttttccgac
    28081 cagataaaca tcgcggaaga tccccgctga ccaccacata tcctggtctt ccacgtaggt
    28141 agagtccgcc cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc
    28201 gctgatgtca aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt
    28261 aacatagact tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg
    28321 ccagccgtcg ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac
    28381 aaacggcaca tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc
    28441 ttccatttgc cacatggcgg ggacggtaat atgcccccag tcagccatta actcagaggt
    28501 gaaggcttct ggtacttgca gcggatggtc aaaaaagtgg aaattccact gaccgcttaa
    28561 gggcagaaac aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata
    28621 tgaaaaaaag tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca
    28681 gcgattcata gggcctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg
    28741 tgtaattgct ggggagttta gtaaaatttt tactaaaaag tagcaggtaa atgcatttac
    28801 tttaactgga tcacgaaaat aaacggcagc actgtccggc aaaaacgatt gccggacagt
    28861 gtgtaagggg atttaacggg tcgtgccgcg cagttttaat ttgctgggaa cgaagactaa
    28921 cagcggcagc gcgcgaccat cgcgggcttt ttcatacacc aggttaacgc cctgacttcc
    28981 catcatctcg gaatggatgc gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg
    29041 gatatcgtta acgctgataa gcgaaatatc ctgtgggatg ttcaggcctc gttcatgaat
    29101 tgcccgcagt acgccgatag caatggaatc ggaagcaaca aacagtgcct tcggatagtc
    29161 ttcccgcgcc agcatttgtt ttgccagttc ataacccgac gaactggaaa aaccgccgcg
    29221 ccagatatcc tcttcgcgta ccacttgttt cagtcggcca tattccgcaa aggcgacctc
    29281 acgaatatcc gccttgccag gctcatcttc accgccaata aaaccaatac gattaacgcc
    29341 ctggttgata tagaagtcga tgatttcttt actgatgcgt gccagatcga tatccaccgc
    29401 atcgtaaccg ctgccgggtt cgtgaaagtc gataaaacag atattgtcgg tcaacgcaca
    29461 ggcggcggcg cgcagggcgg gcgtgggttt gccgacaatt aaaataccgg tgacgttttt
    29521 aatgtctggt aatccgctgt gttcataaca gttggtgagc tcaatgccca gcttttcgca
    29581 ctgagtttca atgccgtggc ggatcgccag atagtaagga tcgttgatct ccagctcctg
    29641 ttggtagctg tagatagcca gaatatggtg ttggttgact gcacctgtct ggagtttacg
    29701 ggcactactg gtcttgtact ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc
    29761 ttctttcaca ttcaatgtcg gatcgtcatt taagaccctg gatactgtcg ccagggatac
    29821 gccagcttcg attgcgatgt cttttagtgt tgccatgttt acctttttta ctttccctga
    29881 tgtgcgcccc cattgtagag gaagtgctta tcgggtcacc tgttttcagt aaataaagcg
    29941 cgtttctttg acgctgtaac ggtaacacag cggctcacga atgtgatcac gcctgcattc
    30001 tccattttta gcctacagga ttgagaaaaa cttttgctat agtttagtaa aattttaccc
    30061 gaagggagga aacaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg
    30121 tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg
    30181 ttgatgtggg ggaaaaaaca ctgcaaaccg tgaccagcct ggtgccgtac tacagcgaag
    30241 aagagctgat ggggaaaacg gtggtggtct tatgcaatct gcaaaaagcg aaaatgcgtg
    30301 gtgaaacgtc ggaatgcatg ctgttgtgtg cggaaaccga tgacggcagt gaaagcgtgt
    30361 tgttaacgcc ggagcggatg atgcctgcgg gcgtacgcgt cgtataaaaa gatcggatgg
    30421 cgacgtcgta tcgccatccg atttgatatt acgcttcttc gacacttact cgcatggccg
    30481 ccagcgcctt acgcgccgct ttgatcacca gttcacactg ttcaatggtc agtgtcagtg
    30541 gcggttcaat gcggatcgtt ttggcgttat tgagcgttcc ggccaccagt acgcgctggc
    30601 ggaacatctc gctggcaaag ttatagccga tttcgttatc aacaaactca attgccatca
    30661 acatcccttt accacgcgct tcctgtacca gatcgggata ttcccgcgcc agttgacgga
    30721 aaccgtccag caacatatcg cctttttgct cagcctgagc cggtaagttc tgctccagca
    30781 acacattgat ggtcgccagc gccgccgcac aggccagcgg gttgccgcca aaggtggtgg
    30841 tatgcaggaa tgggttgtcg aacagaactg aaaacacctc ttcagtggcg atggtcgcgc
    30901 caatcggcat cacgccgccg ccgagcgctt tggcaaggca gaggatatcc ggctgtacgt
    30961 tctcatgctc gcaggcgaac atcttgcccg tgcgccccat gcccgtttgt acttcatcga
    31021 ggatcatcag tgcgccgaac tcatcgcata gcttacgtac ggcggtgaga tagcccggcg
    31081 gcggcagaat tacgccacct tcaccctgaa tcggttcgag gatcaccgca gccacatcat
    31141 caccggtttt tttgcactcg ttaagagccg tgcgcatggc ttcgatattg ccaaacggca
    31201 catgacggaa gcccggcagc agtggcataa acggtttgcg gaaggtcgat ttcgccgtgg
    31261 ccgacagcgc gccaagtgat ttaccgtgga acgcgccgct ggtggcaata aaagtaaact
    31321 tgccgcgcgg tgactggtaa gctttcgcca gcttcagcgc tgcttcaacg gactcggtgc
    31381 cgctattaca gaagaagctg tatttcagtt taccgggcgt tagcgcagca agggttttcg
    31441 ccaacatcgc ccgtaacgga tcgagcagct cctggctgtg cagcggttgt ttcgcaagtt
    31501 gattctgtac ggcggaaacc acaactggat tacggtgccc cacgttgaaa attccaaaac
    31561 ctcccaggca gtcgataaac tcctgtccct gggtgtcgac aagcgtattt aaacctcccg
    31621 cttgccactc tacggctccg taatccccgc cggcggtaac agatttgcga tactctaaaa
    31681 accccggatt gacatgctct ttgaagtatt caatcacctc tcggttaagt gctttcatct
    31741 cctcatgatc cagcgttcgc ttctcaatga gattcagggc gtgggcgctg cacgctaaag
    31801 ccgatgcgct cgaaggtaac ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat
    31861 accgatttaa gtattgcagg gattgcgcca ctccggctgc gatcgcgaaa atcgggataa
    31921 acacaaattc ggttatcatt tgtgcaatat ttaatgctat ctgttaacat ttgttgttat
    31981 tttgcacggt ttttgtcggg tatggatttg atgattgctg cgcaaatgca cgttttcagg
    32041 gcaataattg cctgattttt gggcaagata attaacgttt tcgtatagta acagagtgaa
    32101 agtattttaa atcaaagaat tagatttgtg ctttaatcgc aaattgcgat ctaaatcaaa
    32161 ttaatcggtt aaagataacc gcagcggggc cgacataaac tctgacaaga agttaacaac
    32221 catataacct gcacaggacg cgaacatgtc ttctcatccg tatgtcaccc agcaaaatac
    32281 cccgctggcg gacgatacca ctctgatgtc cactaccgat ctgcaaagct atatcactca
    32341 tgctaatgac acttttgtgc aggtgagcgg ctttaccttg caagagttac aagggcagcc
    32401 gcacaacatg gtgcgtcacc cggatatgcc aaaagcggcg tttgcggata tgtggttcac
    32461 cctgaaaaaa ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa atggtgacca
    32521 ttattgggtg cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca gtggctatat
    32581 gtcgattcgt acccgggcga cggatgaaga gattgcggcg gtggagccgc tgtacaaagc
    32641 gctgaacgcc ggacgtacca gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg
    32701 gctgggtaaa ctgccttcat taccgcttcg ctggcgggcg cgtggagtga tgaccctgat
    32761 gtttatcttg ctggcggcca tgctttggtt tgttgctgcc ccggtggtga cgtatttcct
    32821 ctgtgtgtta gtggtattgt tggcaagcgc ttgttttgaa tggcagattg tgcgcccgat
    32881 agaaaatgtc gcccgtcagg cactgaaggt ggcgaccgga gagcgtaata gtgttgagca
    32941 cctgaatcgc agcgatgagc tggggctgac attacgcgcg gtagggcagc ttggcctgat
    33001 gtgccgttgg ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa atggcagtga
    33061 gacgctggcg aaaggcaccg atgaactgaa cgaacatacc cagcagacag ttgataacgt
    33121 tcagcaaacg gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac
    33181 ggcgtcggct gccgataaac tttctatcac cgccagtaat gcggcagtgc agggtgggga
    33241 ggcgatgacc acggtgatca agacaatgga cgatatcgcc gacagtaccc agcgcattgg
    33301 caccattact tcgctgatta acgatattgc gtttcagacc aatattctgg ccctgaatgc
    33361 ggcggtggaa gcggcgcgtg ccggcgaaca gggcaaaggt tttgcagtgg tggcagggga
    33421 agtgcgtcat ttagccagcc gcagcgctaa tgctgccaac gatattcgca agctgattga
    33481 tgccagtgct gataaggtgc aatccggttc gcagcaggta cacgccgccg gacggacgat
    33541 ggaagatatt gtggcacagg tgaaaaacgt cacccagttg atcgcccaga ttagccattc
    33601 aacgctggaa caggccgatg ggctttccag cctgacccgt gcagtggatg agcttaacct
    33661 gatcacccag aaaaatgccg agctggtgga agagagtgcg caggtgtcgg cgatggtgaa
    33721 acaccgcgcc agccgactgg aagacgcggt gacggtgctg cattaatcgg tatgccggat
    33781 ccggcggttg gagcacaatg cctgatgcga tgctggcgca tcttatcagg cctgcggggt
    33841 gtgtagcggg tgtaggccgg ataaggcgcg tcaagcgtcg catccggcaa ttgcaccgcg
    33901 ccactggcgg gtgcggcgat taatccagtt gcgtaatatc aaaagcggcg cggtcgatca
    33961 ccgcaatgat cttttttatt tgtgcatcac tgatatcgct ctggttgacg tgtaaatcca
    34021 gcaccgcttt aaaattatcc agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc
    34081 caacgcaacg cgctttgatg cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca
    34141 gccactgagc gccttgttcg gtcagcgcaa tctgcttctt accaccttcc tcttcgcgga
    34201 tggtaatcag cgactgctcc tgcagaaaat ccagcgttgg gtagatgacg cccgggcttg
    34261 gggtgtaatt cccctgggtt aggttctcaa tcgctttaat caattcgtaa ccgtggctgt
    34321 catcgcgcga gagaatatcc agaatcacca gacgcaattc accgtgacca aagaaacgtt
    34381 gccgacgacc gccgccgcga ccgtggcgac caccgcagca ttgaccgtgt tcatgctggt
    34441 gaccgtgtcc gcagtgctcg tgttctgact tctcaccttt gcagcagccc tcatggcgtg
    34501 gctggccttc atgtttacaa cacccttcgt gatgatggct catatcagcc tccttttgct
    34561 tgattagata tatctaattt atgtctgatg tgagcattac tgcaagtaca aaaatgatgt
    34621 ttaaacgtgg aagcacctca catccaggcc ggataagacg ttcacgccgc atccggcagc
    34681 attgctatgg cactgaatgc cttcacatac ttcagatata tcagttctac aaatcgcttg
    34741 catttatcat gattaacaat cattatcatt tacgaaaatt atttagatat atctgattgc
    34801 tatcacgaag gcgataacaa tgaataactc cccccgctac ccgcagcgcg ttcgcaatga
    34861 tctgcgcttc cgtgaactga ctgtgttacg cgttgagcgc atcagcgccg gttttcagcg
    34921 cattgtcctc ggcggcgagg cgctggacgg ttttacatcg cgcggctttg acgatcacag
    34981 caaactcttc tttcctcaac ctgacgctca ctttgtgccg ccaacggtaa cggaagaggg
    35041 catcgtctgg ccggaaggac cacgcccacc gtcgcgtgac tatacgccgc tgtatgacga
    35101 actacgccat gaactggcga tcgatttctt tattcacgac ggtggggtcg ccagcggctg
    35161 ggcgatgcag gcgcaaccgg gcgataaact tacggtggca ggtccgcgcg gttcgctggt
    35221 ggtgccggaa gattacgcgt atcagctgta tgtctgcgat gaatccggaa tgcccgcact
    35281 gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt
    35341 tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac cttgatggtt ttaatatcga
    35401 atggctggca catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc
    35461 cgatgattac ttcatctgga taaccggcga aggtaaagtc gttaagaatt taagccgccg
    35521 ctttgaagcg gaacagtatg acccacagcg ggtacgtgca gcggcttact ggcacgcaaa
    35581 gtaactgata tataaaaaga aaaaggctga cgatttctcg tcagcctttg ctatatctgg
    35641 tggcccctgc tggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact
    35701 gagctaaggg gccatggtag cggattataa agtaactccg tgtcgcaatc cagccattac
    35761 cgtgcgcctg ctgtttttat aaacaatgta ttttcaatcc gttatacttt tcctggtgat
    35821 gttagaaagg agtaaatatg gttgaggata ttttggctcc agggttacgg gtcgtgtttt
    35881 gcggtatcaa ccctgggctt tcatccgctg ggactggttt tccctttgct catccagcaa
    35941 atcgcttctg gaaggtgata tatcaggccg ggtttaccga ccgtcagttg aagccgcagg
    36001 aggcacagca tctgctggat tatcgttgtg gcgtcaccaa actggtagac cgtccaacgg
    36061 tgcaagccaa tgaagtttca aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa
    36121 aaattgaaga ttatcagccg caggcgttgg cgattctggg caaacaagca tatgaacagg
    36181 gattcagcca gcgcggtgca cagtggggga aacaaacgct caccattggt tcgacgcaga
    36241 tttgggtgct gccaaatccc agcggtttaa gtcgcgtttc actagagaaa ctggttgaag
    36301 cgtatcgcga gctggaccag gcgctggtag tgcgtgggcg ataaaaaacg ccaccgaaaa
    36361 ggtggcgttt gtgcaaaggg actagtgccg ggtgcggcgt aacgcctgat ccggcctacc
    36421 gattaatcgt ccaggaagct acgcaacact tcagaacggc tcgggtgacg cagtttgcgc
    36481 agcgccttcg cttcgatctg acggatacgt tcgcgggtaa cgtcgaactg tttacccact
    36541 tcttccagcg tgtggtcggt gttcatatcg ataccgaaac gcatacgcag aactttcgct
    36601 tcacgcgcgg tcaggccagc cagcacgtcg tgcgttgccg cacgcaggct ttcggtggtc
    36661 gcagaatcca gcggcagctc gagggtggta tcctcgatga aatcccccag atgcgaatct
    36721 tcatcatcac cgatcggcgt ttccatggag attggctctt tggcgatctt cagcactttg
    36781 cggatcttgt cttccggcat cagcatacgt tcagccagtt cttccggcgt cggttcacgg
    36841 cccatctctt gcagcatctg gcgagaaata cggttgagtt tgttgatagt ctcaatcata
    36901 tgcaccggaa tacggatggt gcgcgcctga tccgcgatag aacgggtgat cgcctgacgg
    36961 atccaccagg ttgcgtaggt ggagaacttg taaccacggc ggtattcgaa tttatcaacc
    37021 gctttcatca gaccgatgtt gccttcctga atcaggtcaa ggaactgcaa gccacggttg
    37081 gtgtatttct tggcgataga aataaccaga cgtaagttcg cttcaaccat ctctttcttc
    37141 gcacggcggg ctttcgcttc accgatggac atacgacggt tgatatcttt aacctgctcg
    37201 atggtcaggc cggtttcttc ttcaatctgc tgcagtttct gcagggcgcg atgcacttct
    37261 tcagagacat cgtgcagttt ttccgaccac ggcttgttca tcgcaattgc cgcgttgaac
    37321 caggtatcgc tggtttcgtt gccggtaaac agggtaatga agtttttctt cggcattttg
    37381 cactgctcaa cgcagagctt catgatcaga cgttcttgcg tacgaacgcg gtccatcatg
    37441 acgcgcatgc tgttgaccag gtagtcaaac tgcttcggca ccaggcggaa ctgtttgaat
    37501 acttcagaca gtttcaggat ctcttcctga gcggcagcgt gactgcgacc tttcgctttg
    37561 atggtgtcac gcgttacaac gtactgagcg cgtagttccg caaatttttc gcgagccagt
    37621 tccgggtcga tgctgttgtc atcatcggcg ctgtcgtcat cgccatcttc ttcgtcttca
    37681 tcttcgtcat cgtccagatc ttcctgggaa agctcagaac cgacgtgagt ggcggtaggt
    37741 gccagatctt cttctgcgtt cgggtcaaca aagccggtga tcagatcgga cagacgcgct
    37801 tcttctgctt caacacgatc gtactgttcc agcagatagg tgatcgcttc cggatattca
    37861 gcaacggagc attgaacctg gttgatcccg tcttcaatac gcttagcgat gtcaatttcg
    37921 ccttcgcggg tcaacagttc aacggtgccc atttcacgca tgtacatgcg taccgggtca
    37981 gtcgtgcgcc cgatttcaga ttccacgctg gaaagcacct gcgcggcggc ttcggcagca
    38041 tcttcgtccg cggtgttttc agccagcatc agatcatcgg catccggtgc ttcttccatc
    38101 acctgaatgc ccatgtcgtt gatcatttgg atgatgtctt cgatctgatc tgaatcgacg
    38161 atatcttccg gcagatggtc attgacctcg gcataggtca gatagccttg ctccttacca
    38221 cgggtgacaa gaagtttcag ctgtgactgc gggttttgct ccataagacg gtatccacac
    38281 ttatttcatg aggttggtgt tgggcgattg acccgatgtc cttcagccgt taacgctgaa
    38341 atccaacggt tgtttaccgc caacgataat tacgagggcg tacttatata tttgccgctg
    38401 cctctcagtg cggctgtcgg gggcttcccg atcgctcttc ggcacttaag ccgttaaatc
    38461 actttttcgc cagttcctgg tttaatgtcc agagctccag gcgttcttcg ttgcttaaac
    38521 catgcgtgcg ctcacgagcg attaactctt cctggcgcag ttcaagcagc gaatcaaaca
    38581 tatggttgag tgagtcggtg aaggtttgct cagcaatatt cttatctgct atatcgtccc
    38641 acatcgacag tttttcaagg gtggcagcat tatttgtacc acgatagtgc tctaaaagtt
    38701 gcccggtggt cagacctggc tgggagagac aagtgttgac cagttctctg aataagccaa
    38761 gtccagggag cttattttca tccagattct caagcggcgg gaccaacgtc gctaattctg
    38821 gattttgcac cagcaaccct ataagtatac gcatggtcgt gcgttttagc tgcggaacag
    38881 ggcgagaaac gccgctctct gccgcttttg gcattaatcg ttcaagctgg ctgtcatcaa
    38941 gtatgcctaa tttgttgcct aattcctgac gaagatatat tcgcagcgtt tcgcccggca
    39001 cttgcgatat caatggcagt gccagcgtac tcaaacgtgc gcgcccgtca ggggtactca
    39061 gatcaacttg cggcatcagg ctgttaaaca gaaatgcgga aagcggcatc gcctgctcca
    39121 tccgcgcttc aaacgcttct ttaccttctt ttcgtaccag cgtgtcaggg tcttcgccat
    39181 ccggcaaaaa cataaagcgt agctgacggc cgtctgtcat gtaaggcagc gccgtttcca
    39241 gcgctcgcca tgcggcatcg cggcctgcac ggtcgccgtc ataacagcaa atgacattgt
    39301 tggtcgcgcg gaacaacagt tgtatgtgat cggcggtggt tgacgtacct aacgacgcaa
    39361 cggcgtaatt aatgccgtat tgcgccagcg ccaccacgtc catatagcct tcgaccacaa
    39421 gcagacgatt gggttcagcg ttatcctgct gcgcttcata aagaccgtaa agctggcggc
    39481 ctttatggaa aatgtctgtt tccggcgagt tcaggtattt gggggtatcg ttgcccagca
    39541 cgcgcccgcc aaaaccaatc acccgaccgc gtttatcgcg aatggggaac atcacccgct
    39601 cgcggaaacg atcgtaactg cgtccctgat cgttagtgac caacatcccc gcatcaatca
    39661 atgactggcg attttctgga ttgccgccaa accgcttcag gacgttgtcc cagccggggg
    39721 gcgcaaaacc aatcgcaaag cgagcgataa cctcgtggct taatccgcgt ttttccagat
    39781 actggcgcgc agacgtggca acaggttgtt gtaaagattg ttggtaaaac gtattcagac
    39841 cgtccatcaa ctgataaagc gtttgcctct gatggcgctc gatctggctg gggccgctgc
    39901 ctgcttcaaa tggcacttca agattgtgca ttgctgccag ctcttcgacc gtttcaacga
    39961 actcgagctt gtcgtagttc atcaggaagt cgatcgcgtt gccgtgcgcg ccacatccaa
    40021 agcagtggta aaactgtttc tcaccgttaa cggtgaatga cggggttttc tcgttgtgga
    40081 atggacaaca cgcgtggaaa ttcttgccct gctttttcag cttcacacgg gcatcgatca
    40141 gatcgacgat gtcagtgcgt gccagcagat cattaatgaa tacgcgtggg attcgtccag
    40201 ccataggccc cgatttttag caattcataa acgaaaataa gccgcgcatt cctttcggaa
    40261 gcacggcctt acaactacaa ctcggtctga tcggagagca acgctctcgg ggaattagta
    40321 cagacgagtg cggcgtgcgt tttcgcgagc cagtttcttc gcgtgacgtt tcactgcaga
    40381 agctttagcg cgcttacgtt cggtagtcgg tttttcatag aactcacgac gacgaacttc
    40441 cgccagaaca cctgcttttt cgcaggaacg cttgaagcga cgcagagcta cgtcgaacgg
    40501 ctcgttttca cgtactttaa ttaccggcat gtgcctctca cctttgatta attcggtttg
    40561 ccgctggcat caacgccagc ttatttcaaa atggtgcgga attttactgc aattgctgct
    40621 gctttgtaaa gcaccgcggc cttttttgaa agggactttt ataagggtga ggagtataca
    40681 cgaggctttc tccaggggcg aacaaagttt tacatcaacc cgcattggtc ctacactgcg
    40741 cggtaataaa gcgaggtaaa acaagtcatg cgtgtactgg gtattgaaac ttcctgcgat
    40801 gaaaccggca tcgccattta cgacgatgaa aaaggtttgt tagccaacca attgtatagt
    40861 caggtgaaat tgcacgctga ctacggcggc gtcgtgcctg aactggcctc ccgcgatcac
    40921 gtgcgcaaaa ccgtaccgtt gatccaggcg gcgctaaagg agtctggttt aacggcaaaa
    40981 gacattgatg ctgtggccta taccgcaggc cctggattag tcggcgcact gctggttggc
    41041 gcaaccgtgg ggcgttctct ggcgtttgcc tggaacgttc cggcaatccc ggtacaccat
    41101 atggaagggc atctgttagc gccgatgctg gaagataacc cgccggaatt tccgtttgtc
    41161 gcgctgctgg tgtccggcgg tcatacgcag ttaatcagcg tgactggcat tggtcagtac
    41221 gagctgctcg gcgagtctat cgatgatgcc gccggtgaag cgtttgataa aaccgcgaag
    41281 ctgctggggc tggattatcc tggcggaccg ttactgtcga aaatggcggc tcagggtact
    41341 gccgggcgct ttgttttccc gcgtccgatg accgaccgtc cggggctgga tttcagcttt
    41401 tctggtctga aaacctttgc ggcgaacacg attcgtgaca acggcaccga cgaccagacg
    41461 cgtgctgata tcgcccgcgc ctttgaagat gcggtggtcg atacgttgat gattaagtgt
    41521 aagcgagcgt tggatcagac gggctttaag cgactggtca tggcgggcgg cgtgagtgct
    41581 aaccgcacgt tacgggcgaa gctggcggaa atgatgaaaa aacgccgcgg cgaagtgttc
    41641 tacgcgcgtc cggaattttg tactgataac ggcgcgatga tcgcctatgc cggaatggtg
    41701 cggtttaaag caggcgcgac ggcggatctc ggcgttagcg tgcgtccgcg ctggccactg
    41761 gcagagttac cggctgcgta aaactatccg gtgccggaga gcaatttccg gcaccgtcct
    41821 cacttaaagc aacaccacgg gcatccacag caagccggta atcaccagca acacgaggaa
    41881 tatcagtcca aaaatcgccc ccagtcgcca gtaatccacc gttggcagat aaccactacc
    41941 gtagtaaatc ggactgggtc cggtggcgta tggtgtaaga atgctcccca gaccaattgc
    42001 cgcgccaacc atcaggcaga acaccggcag cgggatttcc ggcatcgcca gcgcagcggc
    42061 aatcatcatc ggtgcgaggg cggaggtata cgccgtggcg ctggcgaaaa agtagcgcag
    42121 cagataaaac accacaatca acgccaccat caccatcgtt ggcgaataac cgcttaagct
    42181 gcctgctaac agtttgccaa accagctaat aaaaccagtg ttgttgagtc cggtagcgag
    42241 ggtgataagc gaggccagcc agaagaaaac gttccacgcg gctttattac tgacaatgtc
    42301 gtcccaactg ataatgcgca gaagcagcat cagtgccacc acgctgtaac cgaccatcgc
    42361 ggcatcgata taatcaccgc cgaaaatcca cagcaccagc gcgcctacca tcagccccag
    42421 catccgtttt tcacgcgaac agagcgggcc cattgcctgc agttccgtct ctgcccagcg
    42481 cggcacctga tcgcctgact tcagtaccgg cgggtacagc acataagcca gccagggaac
    42541 cagcagcacc agcaaaatac tgagcggcaa catcccgagg aaccagtcgc cccaactcag
    42601 cgtggcgtga gatgcgcttt tcatcagtcc aattaacagc aagtttggtg ccatcgccgt
    42661 caagaaaatg gcgctggtca cgcagtcggc aacaatcccc atccacatga tgtacgagcc
    42721 aatgctgcgc gaactgctgt cgtttggttg tgattgatag agcggtggca ggttacggat
    42781 gatgggatag ataatccccg caccacgcgc cgagttggac ggtgttacag gtgcgaggat
    42841 cagctcggag aacatcaccg catagccgag aaacagcgtg cgatgtccca tctttttcac
    42901 cagaatcagc gcaatgcggc gtcccagccc ggttttttca tagcctgtgc caaacataaa
    42961 ggcggcgaaa atcagccaga taaccgaatt agaaaaaccg gaaaccgccc acgagaggga
    43021 ttttgcagtg aatttaaagc ctggctgggc gagctgttcg gggctgaaca gcagccaggg
    43081 agagagtatg gcgatgatgg agatacccac catcgccacc acggcacccg gcacgggttc
    43141 gaggatcagt ccaacgatca cgccagtaaa aacggcaaag tagagccagg tatgactctc
    43201 cagccctgcg ggaaccggaa ttagagcaat aatggcgatg accgccagcg gcgcaaggta
    43261 tcgccaccat tcagtggaag gtttcatcgt gttatgcctg cgaaaaagga gagggggcga
    43321 gcctctccgg gagggttatt tgatgtaatg gacgtgctcg cagatctctt ccacgatggg
    43381 atcgcggcgt tcggcgaaca gctttttgtt ttcggctatc aggttgttgc cgtgggtatc
    43441 aatagagaca atcagcgggc cgaactcttt gacccggcag acccacagtg actccggcat
    43501 cccgagttct gtccagtgca cttcttcaat ctcttccacc tgggttgccg ccagcaccgc
    43561 gcagcctgcc gggaaaatca catgtagcgc cttgaatttc tggcagcctt cttcggtcag
    43621 cggccccatt ccgcctttgc caaccaccag tttcacgccg gtctgctcga taaattcacg
    43681 ttcaaaactt tccatacgca tactggttgt cgggccaacg gaaaccatct cccatttatc
    43741 gccgtttttg cgcacgatgg ggccagcgtg gaaaatcgct ttgccgttga gatcgtaagg
    43801 gatcggacgt ttcagttcga tcaagcggcg gtgacaaacg tcacggcagg tcaccagcgt
    43861 accggtcagg tagatcacat cgccgacgcg aatatcttgc agatcttcag ctttgatcgg
    43921 ggttgtcagg atctttttca taacgcgctc cgggtgtgag acagattttc aaaggtgaga
    43981 tcggcatgaa ccagcagtgt gccgcgacga tgcgcccagc agccggtaga gacagcaacg
    44041 ccgatggttg acggatggcg ggcggcagat tcgatatgca cgcccatcac tgaactgttg
    44101 ccggtcagcc cttgtggacc aatccccaga cggttgagtc cttcttccag gcgtagctcc
    44161 agctctgccg cttttggatt gggatggcgg ctgccaatcg gacgcaaaat ggctttacgc
    44221 gagagtacgg cggcggtttc caccgaggtg gcgatgccca cgcccaccag tatcggtgga
    44281 caggcgttta cggcgagggt ggagatattt tcgaagacga atttcaccac gccttcgtag
    44341 ccttctgacg gcattaacac tttcgagcgg ccaggtagcg tgcagccgcc gcctgccatg
    44401 taaacttcga tttctgcatc gtcattgtcg gggatgatgt cccaggtgac ccacggtacg
    44461 ccgctaccgg tatttttgcc ggtgtttact tcgtcaaaaa tttctaccgc attgtgacgt
    44521 agtggcgctt tgacggttgc ctcttccacg gcttgtttga gtatgctttg cagctcgcca
    44581 agcagtggga agcgggaacc gactttaacg aagaacataa tctccccggt gtcctgacag
    44641 gcaggacgat tcaggtcaat cgctttttgc atgttgtcga acatcgtatg gtagataatt
    44701 ttccccatcg acgacgtttc ggcatccttt agctgtttta gtttatccac cacgtcatca
    44761 ggcattcggg tagaaatcat ggcggtaaag ttagcgacaa tctctgtcaa cttattcact
    44821 gcctgttgct tattactttc gctcatcatg gcaactccag tttttgtatt tgtctggaaa
    44881 aattcaccgc cacaaactac cactaaaaat gtggttttat attttttgcc accgtgaata
    44941 tgtaattaac agttcgtgaa tcaacgcgaa aataagaatg aatattgcgc cagagcaggg
    45001 aatggcaatg ttttgcgaaa tacactgcgc ttatcacaac tgcggataat taacgatgct
    45061 gaatagctgg cctttagcca aagacttgca ggtactggta gaaatcgtcc acagcgggag
    45121 ttttagcgcc gcagcggcga cgcttgggca gacgcctgca tttgtcacca agcggatcca
    45181 aatccttgaa aatacgctgg caacaacgct gctgaaccgc tccgctcgcg gcgtggcgct
    45241 gactgaaagt ggccaacgtt gttacgaaca tgcgctggaa atcctcaccc aatatcagcg
    45301 actggtcgat gacgtcacgc aaatcaaaac gcgcccggag gggatgattc gtattggctg
    45361 tagttttggt tttgggcgca gccatattgc gccagccatt accgaactta tgcgcaatta
    45421 tcctgagtta caggtgcatt ttgaattgtt cgatcggcaa attgatttag tgcaggataa
    45481 tattgatctg gatattcgta ttaatgacga aattcctgat tattatattg cgcatctgtt
    45541 aacgaaaaat aaaagaatat tatgcgcagc acctgagtat ctgcaaaaat atccgcagcc
    45601 acaatcatta caggaattaa gtcgtcatga ctgtctggtg accaaagagc gcgatatgac
    45661 ccacggaata tgggagttgg ggaacggtca ggagaaaaaa tcggtgaagg ttagcgggca
    45721 tctttcctcc aatagcggcg agatagtgtt gcaatgggcg ctggagggca aagggattat
    45781 gctgcgttcc gagtgggatg tgttgccgtt tctggagagt ggcaaactgg tgcaagtatt
    45841 gccggagtat gcacaaagcg ccaatatctg ggctgtttac cgggagccgc tctatcgcag
    45901 catgaaatta cgtgtctgcg tggaatttct ggcggcatgg tgccagcaac ggctgggcaa
    45961 gcccgatgaa ggctatcagg tcatgtagat ccaccagaaa tcactcggga tccttttcgc
    46021 gctttctttt gaatttcgtc cagatttttg tctcctgacg acgccacaga cgctggatgt
    46081 tgtcatgatg acgcagcagg atcaggcaag agagcatcga aaccgggaag gtgaattgtg
    46141 gcttaaacca ccatacataa aacggagcaa tcagtgcact gacaatcgct cccagcgacg
    46201 agtatccgct caatagcacg gtcagtaacc aggttcccgc cattactccg gtgagatccc
    46261 agccaatggg tgcgatggca ccaaaagcgg tagcaacgcc ttttcctcct ttaaatccga
    46321 agaaaacggg ccagatgtgt ccaagacagg cggcgatggc aattaagcct agccagaagg
    46381 ggctgacacc taattcatac gcgccccaga cgggcaacat tcctttcaga acgtcgaaaa
    46441 tcagtactgc tacggctgct cccttgccac cgatacgtaa cacattggtt gcgcctggat
    46501 tgccggagcc gctggttcgc ggatcgggca gcccacacaa gcggcaaacc agaatggcac
    46561 tggaaatgga gccgcagagg tacgcgatga ggatcattcc aggcgcgatt gcactcataa
    46621 gctgttccgt tttgaaaatt cgtgttcaac gatgaatctg tggataatac gcacatttcg
    46681 ccggaagtgg tatccggtta gccaaaaagc aggcaggacg tgatggatat tgtatttata
    46741 gagcaacttt cggtaatcac cactattggt gtttacgact gggaacagac catcgaacag
    46801 aagttagtgt tcgatatcga aatggcatgg gataaccgta aagcggcgaa aagtgatgat
    46861 gtggcggatt gcctcagtta cgctgacatt gcagaaacgg tggtcagcca cgtcgagggg
    46921 gcgcgttttg cgctggtgga acgcgtggct gaagaggtgg cggagctgct gttagcacgc
    46981 ttcaactcgc cgtgggtgcg tatcaaactc agcaagccag gcgcagtggc gcgggcggcg
    47041 aatgttggcg taatcattga gcgtggcaat aatctgaaag aaaataatta attttacagc
    47101 tgttaaacca aacggttata acctggtctt actgcagtag tccggacaag cggtacattt
    47161 taataattta ggggtttatt gatgagcgat atgcactcgc tgctgatagc ggcaatattg
    47221 ggtgtggtcg aaggattgac agaatttctg ccggtatcca gcacgggcca tatgattatt
    47281 gtcggtcact tgttggggtt cgagggcgac acggcgaaaa cctttgaagt tgtgatccag
    47341 ttaggatcaa ttctggcggt agtagtgatg ttctggcggc gtctgtttgg cctgattggc
    47401 atccactttg gccgcccgtt gcagcacgaa ggagaaagca aaggtcgttt aacgctgatc
    47461 cacattttgc tggggatgat tccggcggtg gtactggggc tgttgttcca cgacacgatt
    47521 aagtcattgt ttaacccgat aaatgtgatg tatgcactgg tcgttggcgg tttgttgctg
    47581 attgccgccg aatgcctgaa gccgaaagag ccgcgtgcgc cgggtcttga tgatatgacc
    47641 tatcgtcagg cgtttatgat tggctgtttc cagtgtctgg cgctgtggcc gggtttctcc
    47701 cgttccgggg ccaccatttc aggtgggatg ctgatgggcg tgagccgtta cgctgcttcc
    47761 gagttctcgt tcctgctggc ggtaccgatg atgatgggcg caacggcgct cgatctctac
    47821 aaaagctggg gcttcctgac aaccggcgat atcccgatgt ttgccgttgg gtttatcacc
    47881 gcttttgtgg tggcgctgat agcgattaaa accttcctgc aattgattaa gcgcatttcg
    47941 tttatcccgt tcgccattta tcgctttatt gtggcggctg cggtgtacgt cgtgttcttt
    48001 tgattgagaa aaggttcggg agcaggcctg gttttctccc tcgctctgta gggagagggc
    48061 cggggtgggg gtgattgttc gcaccggtgc tggtctgttc ccctcaccct aaccctctcc
    48121 ccgaaggggc gaggggatgg ttcgtgttaa cgtttagttt tatactcagt caggctttgg
    48181 gcaacgttgt tccttccaac tggctaccgc cgcaatccgc cgtcgggtca gctcctcgcg
    48241 aatctccaca cctttaaatc ccgcttcaac gaccgctttt gtcggcactg actgcgccac
    48301 ttcccaggct tcgcgcaacc aacggccttg cgggtagtcc gctgattcaa aaccggttct
    48361 gccgcgtacg tcagcctcgc tggtcagcgc cagttgctcg acacgctgcg gtttacgcca
    48421 ggcatcgatg gaatcaaata atttgacgat ggtttttggg ttcagcattg ggaaggtgtg
    48481 gatgagatcg tgaaactcag ccaccagtct ggctaaatcg cgaatttcat ttggcacgcg
    48541 cagacgctgg catagttgtt ccactaactt aacacccgcc gggccatgcc catgatgacg
    48601 cggccaaagt tctggcggcg tcagtccttt accgagatcg tggcataaag ttgcgaaacg
    48661 gacatcaacc tgcggactca gcatcgccgc cattgagagc gtcattaagg tatgaatacc
    48721 cgtgtcgatt tccggatgcc acctggcagg ggccggaacg ccaaacaggg cgtcaatttc
    48781 cgggaataaa acgcgaagtg caccgcaatc gcgcagtacc tggaagaaca cctggggatt
    48841 gcgggtggtg agggcgcttt ccgtctcttt ccatacccgt tcaggcgtca ggtgttccag
    48901 ttcacccgca tgggtcatct cgcgcatcag cgtcagagtt tcatcggcaa tacgaaaacc
    48961 gaggtgggca taacgcgcag caaaacgcgc cacgcgcaga acgcgtaacg gatcttcgcc
    49021 aaaagcgggg gaaacatggc gcaacagacg attttgcaga tcgcccagac cgttgtacgg
    49081 gtcgataatc tcgccgttat cgtcctgagc cagcgcatta atggtcagat cgcgacgctt
    49141 aagatcatct tccagcgtga catccggtgc ggcatagcaa gtaaaaccgg tgtaaccgga
    49201 accggatttc cgttcggtac gtgccagcgc atactcttca tgcgtttgcg gatgcagaaa
    49261 cacaggaaaa tcgcggccta cctgctggta gcccgcgtcg agcatctcct gtggcgtact
    49321 gccgaccacc acccaatctc tgtctttgac cggtagccct aacaatgcat cccgaacagc
    49381 accaccgacc agataaatct tcacgccatc atcccttcgt caaatacaca attcctaata
    49441 ataagacagt gcaacgaaga aggcgattta gttcatccag cgatctttgc gtttgcgcgt
    49501 tgggatcagg tgtggcagta ccagaccgag cagcaagcca agccccagca cgccaccgcc
    49561 atacataaac cattgcatga tgatggtgcg ctgtttgtca tccagctgta cgctggcggc
    49621 atcgaccttt ttctgcgcga caatcagctc gtttttcaat ttctgatttt cttcttttaa
    49681 tccgttgatc acgctgtcgc tctgcgccac tttttgctgc atttctgcgg tgcgctgatt
    49741 ccaggtgtta tcgatattgg tgagtttatc ggtcagggtt ttgacctgat tttccagatc
    49801 tggcacacgg gagcgcaggc ttggctcagt gctaagttgt ttcaacggga tccaggcggt
    49861 acggccagag ctgtctttca cctgggcata attggtgttg gcgtcagttt gtaataaggt
    49921 cacttcctcg ccggcgttaa ccgtgcccac gaggcgataa tgatctcccg gaccgctacg
    49981 gacccaggta ttcagttcat cagaaacata gcgcgtttct tcagcgtgtg agacggcagt
    50041 ggcgctaagt gcgagtaaag ttaatccgat caggcgtaat tttggcatca ggctgtcgtt
    50101 attgtcatgg aaagtggaac gatagtagtg gcatcagtgt cgctacgcaa agcattcgac
    50161 atcaatcgga atcctctgtg tcaggtcttg ccactgcttc aaacttttac gcccgtcaaa
    50221 ttgcccgttg catggcaatt tggcgcaaaa tactatctac tgacaaaaaa gatcgcatta
    50281 agttcgccgt tcatcgtcac ttatgtgaca taaccgataa gtaaaggcca tggctcagga
    50341 aatcgaatta aagtttattg ttaatcacag tgccgttgag gcgttgcgtg accatctcaa
    50401 tacgctgggc ggcgagcacc atgaccccgt gcagttgctg aatatttact acgaaacgcc
    50461 ggataactgg ctgcgtgggc acgatatggg cttacgtatt cgtggcgaaa acggtcgcta
    50521 tgagatgacc atgaaagttg caggaagagt gacaggcggc ttacaccagc gcccggaata
    50581 taacgtggcg ttgagcgaac cgacgctcga cctggcgcag ttaccgacgg aagtctggcc
    50641 gaacggcgaa ttgcccgccg atctcgcttc ccgcgtacaa ccgctgttca gcaccgattt
    50701 ttatcgcgaa aaatggctgg tggcggtcga tgacagccga attgaaatcg ctctcgatca
    50761 gggggaggtg aaagcggggg aatttgctga acctatctgt gagctggaac tggaactgct
    50821 tagcggcgac acgcgcgcgg tgctgaaact ggcgaaccaa ctggtatcgc aaaccggatt
    50881 acgccagggc agcctgagca aagcggcgcg tggctatcat ctggcgcagg gcaatccggc
    50941 gcgtgaaatc aaaccgacca ccattttgca tgttgcggca aaagccgatg tggaacaggg
    51001 gctggaagcg gcgctcgagc tggcgttagc gcaatggcag tatcatgaag aactgtgggt
    51061 acgcggcaac gatgcggcga aagaacaggt gctggcagcc attggtctgg tccgtcatac
    51121 actgatgctg ttcggtggta ttgtgccgcg caaagcgagc actcacttac gtgatttgct
    51181 gactcaatgc gaagcgacca tcgcttctgc ggtttctgcc gtgacggcgg tctactctac
    51241 caaaacggca atggcgaagc tggcgttgac cgaatggctg gtaagcaaag cctggcagcc
    51301 gtttttagat gccaaagcgc agagtaaaat gagcgattcc ttcaaacgct ttgccgatat
    51361 ccatctttcc cgccatgccg ctgaactgaa aagcgttttc tgccagccgt tgggcgatcg
    51421 ctaccgtgac cagttgccac gtctgacgcg tgatattgac tcaatactgt tgctggcggg
    51481 ttactatgat cctgtcgtcg cgcaagcctg gctggagaac tggcaggggc tgcgtcacgc
    51541 tattgcaacc gggcaacgca ttgaaattga acatttccgt aatgaggcaa acaatcagga
    51601 accgttctgg ttgcacagcg gaaaacgtta atcaggcaag gataaaatcg cttatgaagc
    51661 cgctctcttc accgttacag cagtactggc agaccgttgt tgagcggctg ccagagcctt
    51721 tagccgagaa atcacttagc gcacaggcga agtcagtact tactttcagt gattttgtgc
    51781 aggacagcgt gattgcgcat ccagagtggc tgacggaact ggaaagccaa tcgccgcagg
    51841 ccgacgaatg gcagcattac gcggcatggt tgcaggaggc gctcagcaat gtgagtgacg
    51901 aagccgggtt aatgcgcgag ctgcggctat tccggcggcg cattatggtg cgcatcgcct
    51961 gggcgcaaac gctggctttg gttactgaag agagcatatt gcagcagctc agccatctgg
    52021 cggagacgct gattgtcgcc gcgcgtgact ggctgtatga cgcttgctgc cgcgagtggg
    52081 gaacgccgtg caatgcgcag ggcgaagcgc aaccgctgct gattttaggc atgggtaagc
    52141 tgggcggtgg ggaactgaat ttctcctcag atatcgatct gatttttgcc tggccggaac
    52201 atggttgtac gcagggcgga cgccgggaac tggataacgc gcagtttttt acccgcatgg
    52261 ggcagcggct gattaaagtg ttggatcaac caacgcagga tggcttcgtc tatcgcgtgg
    52321 atatgcggct gcgtccgttt ggcgaaagtg gcccgctggt gctgagcttt gccgcgttgg
    52381 aggattatta ccaggagcag gggcgcgact gggagcgtta cgcgatggtc aaggcgcgga
    52441 ttatgggcga tagcgaaggc gtctatgcta acgagttgcg tgcgatgctg cgcccgtttg
    52501 ttttccgtcg ttacatcgat ttcagcgtga ttcagtcgct gcgcaacatg aaagggatga
    52561 ttgcccgtga agtgcgtcga cgtggtttga ccgacaatat caaactcggc gcaggcggca
    52621 ttcgcgaaat tgaatttatc gttcaggtgt tccagctcat tcgcggtggg cgcgaaccgt
    52681 cgctgcagtc gcgctcttta ctgccaacgc tcagcgtcat tgccgcgctg catctgcttt
    52741 ctgaaaacga tgctgaacaa ttgcgagtgg cgtatctgtt cctgcggcgt ctggaaaacc
    52801 tgctgcaaag cattaacgac gaacaaaccc agacgcttcc ttctgatgag cttaaccgtg
    52861 cgcggctggc gtgggcgatg gactttgctg actggccgca actgaccggg gcgctgaccg
    52921 cacatatgac caatgtgcgc cgggtgttta atgaattgat tggcgacgac gaaagtgaaa
    52981 ctcaggaaga gtcgctgtcg gaacagtggc gtgagctgtg gcaggatgcg ttgcaggaag
    53041 atgacactac gccagtgctg gcgcatctta gcgaggatga tcgcaaacag gtgctaacgc
    53101 tgattgccga tttccgcaaa gagctggata agcgcaccat cgggccgcga ggacgtcagg
    53161 tgctcgacca tctgatgccg catctgctaa gtgatgtctg tgcgcgtgaa gacgctgccg
    53221 ttacgctgtc gcgcattacc gccttgctgg tggggattgt tacccgcacc acctatttag
    53281 aattgctcag tgaattcccc gcggcgctta aacatttgat ttctctgtgt gccgcgtcgc
    53341 cgatgattgc cagccaactg gcgcgttatc cattattgct ggatgaattg ctcgatccaa
    53401 acacccttta ccagccgacg gcgaccgatg cctaccgcga tgagttgcgc cagtatttgc
    53461 tgcgcgtgcc ggaagatgac gaagagcaac agcttgaggc gctgcgtcag ttcaaacagg
    53521 cgcagctgtt acgcatcgcc gcagcggata tcgctggtac gctaccggtg atgaaagtga
    53581 gcgatcactt aacctggctg gcagaggcta tgatagatgc cgtcgttcag caggcgtggg
    53641 ttcaaatggt ggcccgctac ggtaagccaa atcacctcaa cgatcgcgaa ggccgtggtt
    53701 ttgcggtggt cggctacggc aagctgggcg gctgggagtt aggctacagt tccgatcttg
    53761 acctgatctt cctccatgat tgcccaatgg atgcgatgac tgacggcgag cgggaaatcg
    53821 acgggcgaca gttttatctg cgtctggcgc aacgcattat gcatctgttc agtacgcgta
    53881 cctcttccgg cattttgtat gaagtggatg cacggctacg cccgtccggg gccgcgggaa
    53941 tgctggtgac atccgcagaa gcatttgccg attatcagaa aaacgaggcc tggacgtggg
    54001 aacatcaggc gctggtgcgt gcgcgggtag tgtacggcga tccgcagctc accgcgcact
    54061 ttgacgcggt gcgtcgcgag attatgacgc tgccgcgtga aggtaaaact ctgcaaacgg
    54121 aagtgcggga aatgcgcgag aaaatgcgcg ctcatctcgg caataaacat cgcgatcgct
    54181 ttgatatcaa agctgatgaa ggcggaatta ccgatatcga atttattacc caatatctgg
    54241 tgttgcgcta cgctcatgaa aaaccgaagt taacgcgctg gtcagacaac gtgcgcattc
    54301 tggaactact ggcgcaaaac gacattatgg aagagcagga agcgatggcg ctgacccgtg
    54361 cttatactac gctgcgcgat gaacttcatc atctggcatt acaggaattg ccgggccatg
    54421 tgccggagga ttgcttcacc gcagagcgtg aactggtgcg ggcaagctgg cagaagtggc
    54481 tggtggaaga atgaagtatg gtattatcgc gcgcaaattt tgaatctctc aggagacagg
    54541 aatgaaagta acgctgccag agtttgaacg tgcaggagtg atggtggttg gtgatgtgat
    54601 gctggatcgt tactggtacg gtcccaccag ccgtatctcg ccggaagcgc cggtgcccgt
    54661 ggttaaagtc aataccatcg aagaacgtcc gggcggcgcg gctaacgtgg caatgaacat
    54721 cgcttctctc ggtgccaatg ctcgcctggt cgggctgacg ggcattgacg atgcggcgcg
    54781 tgcgctgagt aaatctctgg ccgacgtcaa cgtcaaatgc gacttcgttt ctgtaccgac
    54841 gcatccgact atcaccaaat tacgggtact ttcccgcaac caacagctga tccgcctgga
    54901 ttttgaagaa ggtttcgaag gtgttgatcc gcagccgctg cacgagcgga ttaatcaggc
    54961 gctgagttcg attggcgcgc tggtgctttc tgactacgcc aaaggtgcgc tggcaagcgt
    55021 acagcagatg atccaactgg cgcgtaaagc gggcgttccg gtgctgattg atccaaaagg
    55081 taccgatttt gagcgctacc gcggcgctac gctgttaacg ccgaatctct cggaatttga
    55141 agctgttgtc ggtaaatgta agaccgaaga agagattgtt gagcgcggca tgaaactgat
    55201 tgccgattac gaactctcgg ctctgttagt gacccgttcc gaacagggta tgtcgctgct
    55261 gcaaccgggt aaagcgccgc tgcatatgcc aacccaggcg caggaagtgt atgacgttac
    55321 cggtgcgggc gacacggtga ttggcgtcct ggcggcaacg ctggcagcgg gtaattcgct
    55381 ggaagaagcc tgcttctttg ccaatgcggc ggctggcgtg gtggtcggca aactgggaac
    55441 ctccacggtt tcgccgatcg agctggaaaa tgcagtgcgt gggcgcgcag ataccggctt
    55501 tggtgtgatg accgaagagg aactgaagct tgctgtagcg gcagcgcgta aacgcggcga
    55561 aaaggtggta atgaccaacg gtgtctttga catcctgcac gccgggcacg tctcctatct
    55621 ggcaaatgct cgcaaactgg gtgaccgtct gattgtcgct gtgaacagcg atgcctccac
    55681 caaacggctg aaaggggatt cccgcccggt aaacccactc gagcagcgta tgattgtgct
    55741 gggcgcactg gaagcggtcg actgggtagt gtcgtttgaa gaagacacgc cgcagcgtct
    55801 gatcgccggg atcctgccag atctgctggt gaaaggcggc gactataaac cagaagaaat
    55861 tgccgggagt aaagaagtct gggccaacgg cggcgaagtg ctggtgctca acttcgaaga
    55921 cggttgctcg accactaaca tcatcaagaa gatccaacag gataaaaaag gctaaccgga
    55981 aagcggttca cagatctcgc ttcgtagcag gcaggcctat tgacgctgtt gttttttcaa
    56041 cctaaagtaa aggaacaagg gtaagggagg atttctcccc cctctgatca gttgttagta
    56101 agtcgggaaa cttaacagta acaacacaac cagtatgatg acgaacttca tcataaccct
    56161 ttccttctat aaggcccctt cctcgggagg ggctttcccg tttcagcccc ttgctgactc
    56221 cccgattgtt gatgagcgcc ggaccccgcg caacgctttc cctgaagcag ttttgccttc
    56281 cccgagcggt cacgcaaaac gctgcaacgt cctgagcatt tgcacctttt agcggaacgc
    56341 agatataaaa aatccccggc tttttgggcc agggatatgt gttaatgaaa ccttcttgac
    56401 gctgattttt tttcaaccta aagtaaagga acaagggtga gggaggattt ctcccccctc
    56461 tgattggctg ttaataagct gcgaaactta cgagtaacaa cacaatcagt atgatgacga
    56521 gcttcatcat aaccctttcc ttctgtaagg ccccttcctc gggaggggct ttcccgtttc
    56581 agccctttgc tgactccccg attgttgatg agcgccggac ctcgcgcaac gctttcccag
    56641 aggcagtttt gccttccccg agcggtcacg caaaacgttg caacgacctg atcatttgca
    56701 gtttttagcg gaacgcagat ataaaaaatc cccggctttt tgtgccaggg acgttcatag
    56761 ctgaaaattt attctacgtt ttcggcgacg ggagttgttg aggttaaggg tgaagtcaat
    56821 gccgccagtg agccgcctga aacgccaatt tcattgagca aggagtcaat cagcggtgcc
    56881 tgtgtgcggt aagagagagc ggctgataat gcttgttccg ccaggtttcc gccccccaca
    56941 ttacccgtgt ttgcatcacc cgcagcgctg ccacgattca gaccatcgac ctgaataatc
    57001 ttgataccgt caatagattt catcggctca acggattttt ctattaccgc aggtagcgcc
    57061 tgcaacaagg ccagtttgaa tttaaggctg gtttgttcat cagaaagtac gttgatagca
    57121 tcgttcagcg cacgttgtgc ttctgcttcc gccagaccct ttttacgtgt agcttcggct
    57181 aactcaacga tagccgccgc ctgcatctct gcggcttctt tttctgcttt cgcccgcacg
    57241 gtcagttcaa ccgctttggt ttctgcatcc tgcgcggcag cgattagggc aacttgttta
    57301 gcacgatcgg cttctgcggt ctggcgagtg gtttctacgt tttgttgcgc acttactgct
    57361 tctgcgagtg caaggttagc acgcgcttct gcctgggact gttgttccga tttggcggca
    57421 atagcgatcg atttcgtctg gttggcgatt tcggtgacct gctgttgttc gatctcttta
    57481 atacgaactt cacgttcagc ctcaaccttt cttgagcgga cggcctgctc gcgctcgatc
    57541 tccgtttcct gaatttgtcg ttcagccaga attcgcgtct gctctgcttc acgacgacgt
    57601 tcagcttcaa aagccgcaat acgtgcattc tgttcggcag tacgggtttt aacctgctgc
    57661 tcctgctcaa gcgtcataaa cgcttcttgt tgttcaatct ccaacttgcg cgaaagtgca
    57721 tcacggtttt tctcacgcac cgcaacttct acatcctgtt caacttcgtt acgttcgcga
    57781 cgacggcgct ccgtctcctg agtcagtttg gttaaacctt cggcgtcaaa ggcattgttc
    57841 ggattgaaat gttctttcga ggtctggtta aagttggtaa gtgaaacgct ttccagctca
    57901 agaccgtttt tcgacaggtc ttctgcgaca gtattttgta ctccctgcac gaagttctcg
    57961 cgggtatcct gtaactcatg catggtcatt tgtgcagctg ttgcacggag ggcatcgaca
    58021 aatttatctt caaccaacat acgtaagtct tctggtgata gcgtgcgttg ccccaacgtc
    58081 tgcgccgcag tggcgatgcc ttctacagac ggttttaccc gtacaaagaa agcgactact
    58141 acatcgacac gcatacgatc tttcgtaatc aggctatcaa tggttgagcg gctgacttcc
    58201 agcttcagag tattcatatt gatggggatt atttcatgaa agatcggcat cacgattgcg
    58261 ccaccgctca tcaccacttt ttgcccacct aaaccagtac gaacaaaagc ttgctctgcc
    58321 gaagcgcgac gatagagcct ggcgaaaata attccaataa taaacagaat gcatacggca
    58381 ataatcgcgg taaacatcca ggagggcaca gaattaacaa tatcatccat tattaaattt
    58441 ccttttgatt aataaattac aatatttgtg gccagggatt gttttccgcc aaatatcgcg
    58501 ttgcagaaag tcggcaaata attaacactt tgtctccttt agtgaaaaat tttccttctt
    58561 caggctccag tagtaaataa tgtatttggc caaattgatc ggtgagtttt ccttcgcagg
    58621 gattacctga agtggcttga tgaccggtaa ttaatgccat gctaccaata tattcttctt
    58681 ctgtaatagc tgaactatgg tcgcgtggaa tccagggcgc aactatttta ccggtataat
    58741 gcaccgcaat aatcgtaaac agtaaactga caggaaccac gaacaggttc gacagtggtg
    58801 attgccagac catgatgcag gcatgctgca acaggatgcc aataagacca aaaaaaccag
    58861 ccagcaagca gagaacgacg agagctggca atcttccgat attgagataa tgaagtgcct
    58921 ggctaatatg acctgtggtt atggaatcat aatggtcaag atgtgcatca agagcaccgg
    58981 agagcatgtg accgcagata agcgctaaaa tttctaaaag gccgatcaac agtacaaagg
    59041 aaatagcgaa aagataaggg gtattatagt cggcgaataa aatcatatgt cactccaatg
    59101 tccatttagt gacgcaatat ttactttggg atagttttct tgcttacatt tatctttgtt
    59161 aataataaca catgttagag aagttctgga agagaaagtt ttttaatatg gttttttatt
    59221 tttcactaaa aagtgtgatc ggggacaata tatttacgca cgttatgttt aaaggcacta
    59281 cactgattgg ggaatactga aatcagaaaa agcatatggc cttaaaaggt atgcgataac
    59341 cggaggatgt gcttatggat catagtctta attctttaaa taatttcgat ttcctggcgc
    59401 gtagttttgc cagaatgcac gcagaaggtc gcccggtcga tattcttgcc gttactggta
    59461 acatggatga agaacataga acctggtttt gcgcacgtta tgcctggtat tgtcaacaga
    59521 tgatgcaggc aagagagctg gagttagagc actgatataa cgggcctgat ggcccgtttt
    59581 agtgtttgta ttagatgcta gttataggtg attttgagga tgacgtcttt actaaacggt
    59641 ccctcaacaa cagtctctgc cggattcgca ctaagttctg ccctgaatgg tgcggtgacc
    59701 gaaggtgcac tagacgatga aaaagaagca aattgtgaga agcggttaaa ttccgttgct
    59761 gttccatctt tatgaaatat cttcaatagc agtccattac ccatatccag atggctataa
    59821 tcactcaaaa tatccgtagt gaaaaagctc cctaaaatat tgaattgctc agtacaggcc
    59881 gcaccaggat cttttgtcat gactacacta aacgtctcac taacgctggt atttttaatt
    59941 gttcgggaat ttattttacc aaagtcaaca acgccatcag ctggttccag tgcgacatta
    60001 acaaagcagt caaggacatg aatattattt aatccacgaa tcctgaaggt caaatttttg
    60061 tctacttcta tagctcctgc gccactcata cctttaaact ctagagcgat atattcatca
    60121 ggtaacacca ggtttttatt aactctattg gcattttttg ctaatttaat atagagttgt
    60181 acgcgagctg gaaaggttac tgataattga ggctcaggac ctccactaca gacagcgggg
    60241 tttttatagt gattattaaa ggtacccgct ccttttatat caatgcattc tggtgctgcg
    60301 atagcattag gggcagtcga atcataatca actccattgt atgttacgcc aaaaatatag
    60361 gagttctgat tataaaagtc ctgaccgcct ggtaaaaaca tcattatatt agtccaggct
    60421 gatgggtatt cttctccagg tgatggggca ggtaatgcat tattacagaa gacattcata
    60481 aaatactcac tgctttgcca gatcttatca ccttttttac cattactggg gatagaaatt
    60541 cttggtacgt aaccaatgac tacggtattt cccccttcat gttcagcata gcagtccatt
    60601 gcccatacag gtaatgcact aaaacctata ataaatataa ttacaattaa taagtagcgc
    60661 atattattta tctcgtttgg ctacggaaac attacaagag ttattaaccg tcgggcagtt
    60721 tatttcataa aaacttaatc caccaaaatc attgatataa ccaacctgaa aagctgacgg
    60781 aacattttta aactgcgtat ctgcatggct ataaggggtt accattaaac tttgcgtttg
    60841 tggcaacatt gtgattccgt ttgttccgat ataacccagc gtaacgtgat aaggcatctt
    60901 attgttaaag cggatcgaac catcatttct acggatgatt tcaacttttt cgtaagacat
    60961 taccccatca tcttctaatt caatagcttt tggtcgccag aatagtttca atgcgtgctg
    61021 catagtgact tgcatcacat tttttttatc tgtttttggc ggtatttctc gcacgttaaa
    61081 atagaagagc gtttctctgt cattaggcag ttgagcaatt ttttcctgac ttatcattct
    61141 tatttgaatt tgctcactgg gatttaaacg taccataggg ggaaccgtga caataaagtc
    61201 acgtgattta ttgcctttcg tatcttctat ccatgtttgc gccagatagg gtagattttt
    61261 gctttgatta gtaagtttta atgcttcccc tttatctgac tcattcatta tgatacgggt
    61321 acggtccggg atgatggcac cccaggcaat gctgctcatg caggcgagta atactcctgc
    61381 tgcaatgaga ttctttgtgt ttaagtagcg cattagtttc atcctgaatt aattttggct
    61441 aatacacggt aaaataaccg ttccggtggt taagttgctg ctattgggtg gttgaataaa
    61501 acaggattta tcaccccagc gtaaagtaag tttgctgtca tcctgaatgc cgttgaggta
    61561 tacaaagccg ccatcagcaa ccagtcctaa ttctttatgg ctggttttat cttttacgct
    61621 aatccccagt ggtgggtgac taccatctgc caggcgaata acccccatga tttgatagcc
    61681 cttgcgggta tcaagtttgg cgtagccaat agcaccttcg gttaaggtgg tggtgataac
    61741 cgagttttcg atatccacat tttctggaag atttcgggta tcaacgctta atgatgttgt
    61801 tatgtaactg ctgactgatg gaataactcc gatgccatac cgattagtta ccgcgcgttt
    61861 attgttcaac ggaatatctt ctgcaccatc tgcgtcgatc ataaaacgcg agtcagcact
    61921 accactatag tcatggaaag cggctccgtg acgagtcgcc gtgaatgaac cattccaact
    61981 ggcattcaga gaataataac tgtctcgctg tgatgtcccg gagagataga gacgaccgtt
    62041 ctcggtatta tgctgataac tggcgcgtag tgacgctttc atatctttga attcatcatt
    62101 gtcgcctgaa gcagaaacgc tccaggaatt atttcgatcg gaagagtcat accacgaagc
    62161 agtatgcgaa atcttgttat cctgatttcg ttgcatacca tagctcaatg ttctactggt
    62221 tccccaggga atcgaaatat ttaggtacag ttgatcctgg ttgtcttctt cccagtttat
    62281 tcggctaaat gttagagaag tggagacgtt ttttaatggc ccgatatcaa aatcgcggct
    62341 aagtgataat gaataattca cattactgct ggcgtcccag taagtattac gagccaaact
    62401 tacgtatgta tttaacctta atgattcaag atactggtta aacgtgacaa tataactctc
    62461 tttttcattc tgataattcg tgtaatggtt agtgtcattg atatattcat tcattgtcac
    62521 aaagttttta tcagagaaac ggtaaccagc gaaagtgagc tggctaccgg tagattcaaa
    62581 acgttttgaa tagttagcgc ggtagctata acccgtttct ttatcctgat tatgcaactg
    62641 agcatcagat cgtgtgacat caaaagataa tgaacccaat gagttaaggt taaagccaac
    62701 gccggcagcc agagattgat aatcacggtt tgttaaaact gaaccaccat acagggatac
    62761 attgttcagc caaccccatg agaattcacc agtccagaag aagggttgtt gtaaggaatc
    62821 accaccaacg ctggtcggtt tacccatagc agtggtgtag cgaatctgcc ctttacgcgt
    62881 cagataagga atactattag atgcaacttg ccattgcgtg acttgcccgt cctcttcttc
    62941 aaccttgacg tccagttgcc cctgtaatgt attgaataaa ttagtaatag caaatgggcc
    63001 aggaggaaca ttttcttgat aaataatacg gttgttctgt gagacagtga cctttgcatt
    63061 cgtttgtgca acacccgtga tttgcggtgc gtaaccacgt agatcaggcg gcaacatatt
    63121 ctcatcgctt ttcaacgaaa cgccagaaaa atggaaagag tcgaaaatag aggattcagt
    63181 atcgtattga cctatagtta gttttgcatt aattgctggg attgggcgaa acaaataaat
    63241 acgcggaaaa tccaggttgt tcgcctggct tttgccattg ttaaatgact gatcgtactg
    63301 gtaatcagta cgcaggcgcc aggccccaag attaacccca gcctgcccat aggaacttat
    63361 attttgtgaa ttatcgccct gatgtggtgc ataatgagag gcatataaat tgtagtcaag
    63421 aaatgcccca gcaataccat gatcccaaag ttcaggaggc gtccagtttg catctttgta
    63481 ttttaaccat gcctgaggtg cagatatgga taattgcatt ttccctttat cgagataagt
    63541 tgtaatttgt ttttcttttt cgatagggta acaaccatcg atcaggtttt gctgagccac
    63601 ttcaataatc tcatcagtaa agcctaataa tgttaattgc tctggagtca gacaaactgc
    63661 agtttggtta tcaattaata cccattgcag gtccagatgg attggcgtcc ttttattatt
    63721 aataagagcg tttaatgagt atttacctac gggtaattgg ccatcagttt caaattggga
    63781 aaggttaacg ttttcacgat cttcggcttc gattaaatct ttgttgaatt caacggcata
    63841 agctgacgca caaccaatga tgagaacgga taatgaacaa taaatatttt taatcaattc
    63901 gcttattgtg gttaatttta attttttata catctgttcc acttatcatt aatccgttaa
    63961 tgatgtggcc gggcgaaccc ggcctaatgt gattactcgt aagcaatctt atagtcagcc
    64021 tgagcgtaaa cgttgcccgg ggtagcatct ttgcttgggg attccatacg agctttgaag
    64081 ttcagttcct gatagctgtt agtattgttg aatgtaatta ctaccggagt acctaaagtc
    64141 acttcgccgc tctggctatc cagaatacgt acgccaacgc ctgtggcacc accgatttca
    64201 gtttctttgg tgtttttcag caggtcagtc tgacccgtca tagtattcgc tgtggtgaaa
    64261 gtaaccttcg cttttgaata agtggttcca ccctggctca acatgcagtc ttgcaggtga
    64321 atagtgaaag gctcagccaa agatttctgg tcgcttttca gaacggtatc tgctacttca
    64381 cccagattta cagttaagtc ttcgtcacct ggtttaattg aacatggagc agagataact
    64441 tcacctttaa atttaacaat gccgtgacct tcgtcagcaa atacgttatt ggaaagaatc
    64501 attgctacgc cagcaacaag aagtgacttt tta
//
LOCUS       DAZFYC010000011        58093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFYC010000011 DAZFYC010000000
VERSION     DAZFYC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.011"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(195..1094)
                     /gene="pgrR"
                     /locus_tag="ACHKW5_000865"
     CDS             complement(195..1094)
                     /gene="pgrR"
                     /locus_tag="ACHKW5_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO7510302.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1232..2152
                     /locus_tag="ACHKW5_000866"
     CDS             1232..2152
                     /locus_tag="ACHKW5_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7510303.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2152..3084
                     /locus_tag="ACHKW5_000867"
     CDS             2152..3084
                     /locus_tag="ACHKW5_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7510304.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTFGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGVTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            3102..3740
                     /locus_tag="ACHKW5_000868"
     CDS             3102..3740
                     /locus_tag="ACHKW5_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7510305.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            4049..4777
                     /gene="mpaA"
                     /locus_tag="ACHKW5_000869"
     CDS             4049..4777
                     /gene="mpaA"
                     /locus_tag="ACHKW5_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO7510306.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYKTPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4752..5717)
                     /gene="ycjG"
                     /locus_tag="ACHKW5_000870"
     CDS             complement(4752..5717)
                     /gene="ycjG"
                     /locus_tag="ACHKW5_000870"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO7510307.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5836..6342
                     /gene="tpx"
                     /locus_tag="ACHKW5_000871"
     CDS             5836..6342
                     /gene="tpx"
                     /locus_tag="ACHKW5_000871"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO7510308.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(6386..7927)
                     /gene="tyrR"
                     /locus_tag="ACHKW5_000872"
     CDS             complement(6386..7927)
                     /gene="tyrR"
                     /locus_tag="ACHKW5_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO7510309.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(8075..9136)
                     /gene="ycjF"
                     /locus_tag="ACHKW5_000873"
     CDS             complement(8075..9136)
                     /gene="ycjF"
                     /locus_tag="ACHKW5_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO7510310.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(9133..10530)
                     /gene="ycjX"
                     /locus_tag="ACHKW5_000874"
     CDS             complement(9133..10530)
                     /gene="ycjX"
                     /locus_tag="ACHKW5_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO7510311.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10685..11683
                     /gene="ycjW"
                     /locus_tag="ACHKW5_000875"
     CDS             10685..11683
                     /gene="ycjW"
                     /locus_tag="ACHKW5_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7510312.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11794..12699)
                     /gene="ompG"
                     /locus_tag="ACHKW5_000876"
     CDS             complement(11794..12699)
                     /gene="ompG"
                     /locus_tag="ACHKW5_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO7510313.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12744..13826)
                     /locus_tag="ACHKW5_000877"
     CDS             complement(12744..13826)
                     /locus_tag="ACHKW5_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7510314.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKEFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13840..14499)
                     /gene="pgmB"
                     /locus_tag="ACHKW5_000878"
     CDS             complement(13840..14499)
                     /gene="pgmB"
                     /locus_tag="ACHKW5_000878"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO7510315.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(14496..16763)
                     /gene="ycjT"
                     /locus_tag="ACHKW5_000879"
     CDS             complement(14496..16763)
                     /gene="ycjT"
                     /locus_tag="ACHKW5_000879"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO7510316.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16760..17815)
                     /gene="ycjS"
                     /locus_tag="ACHKW5_000880"
     CDS             complement(16760..17815)
                     /gene="ycjS"
                     /locus_tag="ACHKW5_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7510317.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     MLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17825..18613)
                     /gene="ycjR"
                     /locus_tag="ACHKW5_000881"
     CDS             complement(17825..18613)
                     /gene="ycjR"
                     /locus_tag="ACHKW5_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7510318.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(18631..19683)
                     /gene="ycjQ"
                     /locus_tag="ACHKW5_000882"
     CDS             complement(18631..19683)
                     /gene="ycjQ"
                     /locus_tag="ACHKW5_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO7510319.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19714..20556)
                     /gene="ycjP"
                     /locus_tag="ACHKW5_000883"
     CDS             complement(19714..20556)
                     /gene="ycjP"
                     /locus_tag="ACHKW5_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7510320.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(20543..21424)
                     /gene="ycjO"
                     /locus_tag="ACHKW5_000884"
     CDS             complement(20543..21424)
                     /gene="ycjO"
                     /locus_tag="ACHKW5_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7510321.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     HLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(21445..22737)
                     /gene="ycjN"
                     /locus_tag="ACHKW5_000885"
     CDS             complement(21445..22737)
                     /gene="ycjN"
                     /locus_tag="ACHKW5_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7510322.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILLAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(22751..24430)
                     /gene="ycjM"
                     /locus_tag="ACHKW5_000886"
     CDS             complement(22751..24430)
                     /gene="ycjM"
                     /locus_tag="ACHKW5_000886"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO7510323.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(24642..24956)
                     /gene="pspE"
                     /locus_tag="ACHKW5_000887"
     CDS             complement(24642..24956)
                     /gene="pspE"
                     /locus_tag="ACHKW5_000887"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO7510324.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(25031..25252)
                     /gene="pspD"
                     /locus_tag="ACHKW5_000888"
     CDS             complement(25031..25252)
                     /gene="pspD"
                     /locus_tag="ACHKW5_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO7510325.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(25261..25620)
                     /gene="pspC"
                     /locus_tag="ACHKW5_000889"
     CDS             complement(25261..25620)
                     /gene="pspC"
                     /locus_tag="ACHKW5_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO7510326.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25620..25844)
                     /gene="pspB"
                     /locus_tag="ACHKW5_000890"
     CDS             complement(25620..25844)
                     /gene="pspB"
                     /locus_tag="ACHKW5_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO7510327.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25898..26566)
                     /gene="pspA"
                     /locus_tag="ACHKW5_000891"
     CDS             complement(25898..26566)
                     /gene="pspA"
                     /locus_tag="ACHKW5_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO7510328.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26733..27710
                     /gene="pspF"
                     /locus_tag="ACHKW5_000892"
     CDS             26733..27710
                     /gene="pspF"
                     /locus_tag="ACHKW5_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO7510329.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFIFLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27830..29095)
                     /gene="puuE"
                     /locus_tag="ACHKW5_000893"
     CDS             complement(27830..29095)
                     /gene="puuE"
                     /locus_tag="ACHKW5_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO7510330.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(29133..30413)
                     /gene="puuB"
                     /locus_tag="ACHKW5_000894"
     CDS             complement(29133..30413)
                     /gene="puuB"
                     /locus_tag="ACHKW5_000894"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO7510331.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(30415..31902)
                     /gene="puuC"
                     /locus_tag="ACHKW5_000895"
     CDS             complement(30415..31902)
                     /gene="puuC"
                     /locus_tag="ACHKW5_000895"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO7510332.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(32177..32734)
                     /gene="puuR"
                     /locus_tag="ACHKW5_000896"
     CDS             complement(32177..32734)
                     /gene="puuR"
                     /locus_tag="ACHKW5_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO7510333.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32761..33525)
                     /gene="puuD"
                     /locus_tag="ACHKW5_000897"
     CDS             complement(32761..33525)
                     /gene="puuD"
                     /locus_tag="ACHKW5_000897"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO7510334.1"
                     /translation="MENIMNNPVIGVVMCRNRLKDHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33737..35155
                     /gene="puuA"
                     /locus_tag="ACHKW5_000898"
     CDS             33737..35155
                     /gene="puuA"
                     /locus_tag="ACHKW5_000898"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO7510335.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            35304..35468
                     /locus_tag="ACHKW5_000899"
     CDS             35304..35468
                     /locus_tag="ACHKW5_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510336.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            35458..36843
                     /gene="puuP"
                     /locus_tag="ACHKW5_000900"
     CDS             35458..36843
                     /gene="puuP"
                     /locus_tag="ACHKW5_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO7510337.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            36977..37222
                     /gene="ymjA"
                     /locus_tag="ACHKW5_000901"
     CDS             36977..37222
                     /gene="ymjA"
                     /locus_tag="ACHKW5_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO7510338.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            37535..39178
                     /gene="sapA"
                     /locus_tag="ACHKW5_000902"
     CDS             37535..39178
                     /gene="sapA"
                     /locus_tag="ACHKW5_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO7510339.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            39175..40140
                     /gene="sapB"
                     /locus_tag="ACHKW5_000903"
     CDS             39175..40140
                     /gene="sapB"
                     /locus_tag="ACHKW5_000903"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO7510340.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            40127..41017
                     /gene="sapC"
                     /locus_tag="ACHKW5_000904"
     CDS             40127..41017
                     /gene="sapC"
                     /locus_tag="ACHKW5_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO7510341.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            41017..42009
                     /gene="sapD"
                     /locus_tag="ACHKW5_000905"
     CDS             41017..42009
                     /gene="sapD"
                     /locus_tag="ACHKW5_000905"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO7510342.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            42011..42817
                     /gene="sapF"
                     /locus_tag="ACHKW5_000906"
     CDS             42011..42817
                     /gene="sapF"
                     /locus_tag="ACHKW5_000906"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO7510343.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            42885..43238
                     /gene="ycjD"
                     /locus_tag="ACHKW5_000907"
     CDS             42885..43238
                     /gene="ycjD"
                     /locus_tag="ACHKW5_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO7510344.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            43606..44394
                     /gene="fabI"
                     /locus_tag="ACHKW5_000908"
     CDS             43606..44394
                     /gene="fabI"
                     /locus_tag="ACHKW5_000908"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO7510345.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            44539..45666
                     /gene="yciW"
                     /locus_tag="ACHKW5_000909"
     CDS             44539..45666
                     /gene="yciW"
                     /locus_tag="ACHKW5_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO7510346.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            45734..47668
                     /gene="rnb"
                     /locus_tag="ACHKW5_000910"
     CDS             45734..47668
                     /gene="rnb"
                     /locus_tag="ACHKW5_000910"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO7510347.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSEDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(47746..47850)
                     /locus_tag="ACHKW5_000911"
     CDS             complement(47746..47850)
                     /locus_tag="ACHKW5_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510348.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            47904..49889
                     /gene="pdeR"
                     /locus_tag="ACHKW5_000912"
     CDS             47904..49889
                     /gene="pdeR"
                     /locus_tag="ACHKW5_000912"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7510349.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(49871..49999)
                     /gene="ymiD"
                     /locus_tag="ACHKW5_000913"
     CDS             complement(49871..49999)
                     /gene="ymiD"
                     /locus_tag="ACHKW5_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO7510350.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            50036..50209
                     /gene="yciZ"
                     /locus_tag="ACHKW5_000914"
     CDS             50036..50209
                     /gene="yciZ"
                     /locus_tag="ACHKW5_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO7510351.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            50299..51048
                     /gene="yciT"
                     /locus_tag="ACHKW5_000915"
     CDS             50299..51048
                     /gene="yciT"
                     /locus_tag="ACHKW5_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO7510352.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            51317..51535
                     /gene="osmB"
                     /locus_tag="ACHKW5_000916"
     CDS             51317..51535
                     /gene="osmB"
                     /locus_tag="ACHKW5_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO7510353.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(51661..51987)
                     /gene="yciH"
                     /locus_tag="ACHKW5_000917"
     CDS             complement(51661..51987)
                     /gene="yciH"
                     /locus_tag="ACHKW5_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO7510354.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(51987..52724)
                     /gene="pyrF"
                     /locus_tag="ACHKW5_000918"
     CDS             complement(51987..52724)
                     /gene="pyrF"
                     /locus_tag="ACHKW5_000918"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO7510355.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CSLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(52917..54086)
                     /gene="lapB"
                     /locus_tag="ACHKW5_000919"
     CDS             complement(52917..54086)
                     /gene="lapB"
                     /locus_tag="ACHKW5_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO7510356.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQIYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(54093..54401)
                     /gene="lapA"
                     /locus_tag="ACHKW5_000920"
     CDS             complement(54093..54401)
                     /gene="lapA"
                     /locus_tag="ACHKW5_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO7510357.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(54550..55314)
                     /gene="pgpB"
                     /locus_tag="ACHKW5_000921"
     CDS             complement(54550..55314)
                     /gene="pgpB"
                     /locus_tag="ACHKW5_000921"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO7510358.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            55484..56074
                     /gene="ribA"
                     /locus_tag="ACHKW5_000922"
     CDS             55484..56074
                     /gene="ribA"
                     /locus_tag="ACHKW5_000922"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO7510359.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(56138..>58093)
                     /gene="acnA"
                     /locus_tag="ACHKW5_000923"
     CDS             complement(56138..>58093)
                     /gene="acnA"
                     /locus_tag="ACHKW5_000923"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO7510360.1"
                     /translation="PVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKF
                     VEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEK
                     YAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELE
                     VNATHKNRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLG
                     LKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIE
                     TAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEPIG
                     HDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYG
                     WQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAG
                     RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVV
                     SIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNL
                     IGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCR
                     CRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        13985 a        15326 c        14426 g        14356 t
ORIGIN      
        1 ataatcgacg ccaaaatgcg gcaaagcaca tagaacaccg aacactacgt tcaggcttcg
       61 cacgttcaaa ccagttttcg gtatcaaaat cagcatctgt cgccattgta cgaagaataa
      121 aaaataacca aaacgttgaa aggaactgag aatacgttaa gagagaaatt aaattaatgt
      181 atcagcaagt agtgctaaac agcaccttta tacttcagcc tatctataaa taatgagaat
      241 gccgagccag catgtcgacg gtgtggatag tacaggtgat aaccgggtaa atctggtgtg
      301 aatttatcca aaacacgtat cagttttttt tctttaatag cccgctcaac ctgatcataa
      361 ggtagatacg ccaatccatg cccatcaatt gcagcatcaa tgatcaggtc tatcgtattc
      421 agtaaaagct gaccttccat gcgaacacga acttcacgtc caccgcgtat taatctccag
      481 cgatttgctg tacccgatgt gggaagatac aaattaattg cctgatgatc tattaattgt
      541 gacactgacg ttggaacact tcggcgagaa aaataatccg gtgagccaac aatagccatt
      601 ggtatatctg gcccgattcg aatggcgatc atatctttat ccatctcccc acccagacgg
      661 acgcctgcat caaaacgttc agaaacgaca tcggtcaaac cataatcaat ggtgagctga
      721 atatcaattt caggatgcga tttcaggaat gtgcgcattg ctggtaacaa tatcgttttt
      781 gctgcatgtt ctacagtagt aatacgtatt gtcccggatg ggcggttctg cagatcgctc
      841 agggatgcca tggctgaatc tatgtcatgc aacatagggc caagaacaga caaaagatgc
      901 tcgcccgctt cagttggaac aacgctgcgc gtggttcgcg tcagaagccg caatcccaat
      961 cgttcttcta tacgacgcac tatctggctt aaagctgact gcgccatgct caggcgggct
     1021 gctgcacgag tgaagctacg ctcctctgca acgacgacaa acgccatcag atcagcgatt
     1081 tcttcacgct tcattcagta tctccatgca atctattaat catttaaatg ataggtctaa
     1141 tcatgatttt gggtctaatc aacttaatga tcaatatcta tagtgatatc accaaccgtt
     1201 aaaacgaacc gacaactcca tgaggaacgg aatgatgaat aataaagtca gcttcactaa
     1261 cagcaataat ccaaccatct ctttgtctgc agtgatctat ttccctccaa aatttgatga
     1321 aacccgtcag tatcaggcaa ttgtgttatc tcacccaggt gggggcgtta aagaacaaac
     1381 ggccggaacc tatgccaaaa aactggcgga aaagggattt gtcactattg cttatgacgc
     1441 atcttatcaa ggtgaaagtg gcggcgagcc gcgtcagtta gaaaacccat acattcgtac
     1501 tgaagacatc agtgccgtga ttgattacct gacaacgctt tcttatgtcg acaatacccg
     1561 aattggtgcg atgggaattt gtgccggtgc aggatataca gccaatgctg caattcagga
     1621 tcgccgcatc aaggccatcg gtaccgttag tgcggtcaat attggttcga tgttccgtaa
     1681 tggttgggaa aacaatgtga aatctattga tgccctgcca tacgttgaag cgggttcaaa
     1741 cgcccgaacc agcgatatta gcagcggaga atatgccgtt atgccactgg caccaatgaa
     1801 ggagtccgat gcgccgaacg aagaactgcg tcaggcctgg gagtattacc acacccctcg
     1861 cgcgcagtat ccaacagcac cgggttacgc tactttgcgc agccttaacc agattattac
     1921 ctatgatgct taccatatgg cggaagtgta cctgactcag ccgatgcaga ttgtggcggg
     1981 cagccaggca gggagcaaat ggatgagtga cgacctgtac gatcgggcat caagccagga
     2041 taaacgctat cacattgttg aaggcgcaaa ccatatggat ctttatgacg gcaaagccta
     2101 tgtggctgaa gcaatttcag tattagctcc gttctttgag gaaactctgt aatgaaacat
     2161 attcaaatcc gcaatagtga tatggcctgg catattgctg ccaatattca gtttccacca
     2221 aattttgatg agtcaaaaca gtatccggcg attatcagcg tccatccttt tggcagttgt
     2281 aaggagcaga cgtttggcaa tatctatggc aaagcattgg ctgaaaaagg ctatctcgtt
     2341 ctcgcttatg acgccagttt ccagggagaa tctggtgggg agcctcgctg gatagaagat
     2401 ccgacacagc gcgtagagga tattagccgt gttatcgatt atgcagtaac tctgccatat
     2461 gttaatgctg aacgtattgg cgtgctggga gtgtgtgggg gtggtggcta tgccattaat
     2521 gccgcgctga cagaaaaacg aattaaagca gtagtgagta ttacgggtgt taatatcggg
     2581 cgtctttttc gggaaggttt cagcaattac gatccgattg gcgcactgaa tgcaatggcg
     2641 tcgcaacgaa ccaaagaggc tcgtggtgga gaattacaga tcaacgaatt acttcccgcc
     2701 agcctggatg cagcaaaagc ccatggagtg accgaacgcg atgtttatga agcaactgac
     2761 tactataaaa caccacgcgg gcaacaaccg ggaggtgcga caaaaatgtt gttctcacat
     2821 gcacagaaaa cgctggcctg ggatgccttc gcatttactg aagtgttact gactcagccg
     2881 gtgatggtag tagttggtga aaaagtcggt gccttcggtg cttatcgtga tggactggaa
     2941 gtctatggcc gggcgatggt ttcgcaggat cgtcagttag tttcattacc cgatttctca
     3001 cattatgaac tgtatgacaa accggaagca gttcaggagg cgctggaaaa agttattcct
     3061 tttttcaaca cccatctggg ataagtttca tgagaaatat tatgaaaaat gtactgattc
     3121 ttggtgctgg cggtcagata gcccgccatg tgattaatca actggcagat aaacaaacca
     3181 ttaaacaaac tttatttgcc cgtcagccag caaaaatcca caaaccctat ccaactaata
     3241 gcaagattat tatgggagat gtactgaatc atgcagcact gaagcaggcc atgcagggac
     3301 aggacgttgt gtatgccaac cttacggggg aagatttaga tattcaggca aattcagtga
     3361 tcgctgcgat gaaagcctgt gatgttaaac gtctgatctt tgttctttcc ctcggtattt
     3421 atgatgaagt tccaggaaaa tttggcgaat ggaataatgc agttattggt gagcccctaa
     3481 aaccattccg cagggctgcg gatgcaattg aggcttctgg actggaatat acaattcttc
     3541 gccctgcctg gctgactgat gaagatatta ttgattatga gcttacctca cgcaatgaac
     3601 cattcaaagg tacgatcgta tcacgtaaga gtgtcgcagc attaatcaca gatatcatcg
     3661 ataaacctga gaaacacatt ggtgataata taggtatcaa tcaacccgtg actgatggtg
     3721 acaagccatt attcatgtaa atcaattaca tagttaccat cgtaatgtac tatgtgcagt
     3781 acgatttatt aaacttatta aggtgaactg atcaatccga tgcgattaac atgtgtagaa
     3841 ccagtaagaa taaaatacgc catgtatata acattacgac ttacatctaa gtcagcataa
     3901 ttctaatatc tgcacatcaa taaaaatgca aacaacttcc ggcaaaaaag tgatacaaga
     3961 tcgatgacat agcgttgaag aaaaggattt gataatccgc aaaatttgca caacactgcc
     4021 tgtaatactt cctgaaccag agacgatcat gaccgtaaac cgcccacgcg ccgaacgcgg
     4081 cgcatttccg cccggaacag aacattacgg acgttcatta ttgggcgcgc cgttaatctg
     4141 gtttcctgcc cctgcagcca gtcgcgaaag tggtttgatt ctggctggca ctcacggtga
     4201 tgaaaactct tcggtcgtca cgctctcctg tgctctgcgg actttgacac cttctttgcg
     4261 ccgccatcat gtggtgctgt gtgtgaatcc tgacggctgc cagctggggt tacgggccaa
     4321 tgctaatggt gtggatttaa acagaaactt tccggcggcg aactggaagg aaggtgaaac
     4381 ggtttatcgc tggaacagcg ccgctgaaga acgtgatgtc gtattactga caggtgacaa
     4441 acctggctca gaacctgaaa cccaggcgct gtgccagctt atacaccgca ttcaacctgc
     4501 ctgggtggtc tcattccacg atccactggc ctgtattgaa gatcccagac acagcgaatt
     4561 aggcgaatgg ctggcccagg cgtttgaatt gcctctggta accagcgtcg gttataaaac
     4621 gcctggctct ttcggcagtt ggtgtgcaga tctcaacttg cattgcatca ccgcagagtt
     4681 tccgcccatc tcctccgacg aagccagcga aaaatatctc tttgcgatgg caaacttgct
     4741 gcgctggcat cctaaagatg caattcgccc gtcgtgaact gaagcgccgg ttccacatct
     4801 accgccagcc aggtcggtcc gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa
     4861 gcggcgctaa tggcacgaga ggtacacaac atgcagccca gcatcagact gaaaccttgt
     4921 gcacgcgctt cagtcgccag cgccagcgct tccgtcagac caccggtttt atcgagctta
     4981 atgttaacca tctcatagcg ccctttcagc gccttcagat tgctacgtgt atgacaactc
     5041 tcatcagcac aaatcggcaa cggatgaata aaattctcca gcgccgcatc gtcctgcgca
     5101 ggaaggggtt gttcaagcat cgcaacgcct aaatccgcca atagctggca acgcgccgcc
     5161 aacccttctg cacgccagga ttcatttgca tcaacgatca gcgtcgcatc gggcacagct
     5221 gtgcgaattg ccaccatccg ctcactaata agatggttat ccagcttcac tttcagtaat
     5281 ttcgcgcctg cctgccagag tgttgatgca ctattggcca tctgatcagg cgtaccgatg
     5341 actaccgtct gtgcagtgat aactgtctcg ggaagcgtta tgccgatcaa atcagccagc
     5401 gattgctgct gttttcgcgc ggccagatcc cacaatgcac aatccagcgc attacgtgct
     5461 gcgccggcag ggagaatttt ttgcaactcc tcccgtgtca gccctttctc taattgcgac
     5521 acgacgctca taatttgcgc cattaccgag gcatcacttt ctccataacg cggatacggc
     5581 gtgcattcgc cggtgccttt aataccctct tcttccagtt caaccaccac cacgcgcgct
     5641 tcactgcgac ttccccgggc aatcacaaac ggggtatgta agggccaggc ttcctcaaat
     5701 accttaacgg ttctcatctt tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa
     5761 tagcgtttgc tgtgttattg acagttagca taaactaggt gtgacgttaa ctatatgtaa
     5821 acaggaaaga taatcatgtc acaaaccgtt catttccagg gcaacccggt tacagtcgcc
     5881 aattccatcc cgcaggcggg tagcaaagcg cagactttta ctctcgtagc aaaagatctg
     5941 tctgacgtca ccctcggtca gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt
     6001 attgataccg gagtttgcgc cgcatcagta cgtaagttta accaactggc aaccgagatc
     6061 gacaacaccg ttgtgctgtg tatttctgcc gatctgccgt tcgcccagtc tcgtttctgc
     6121 ggcgcagaag gtctgaacaa cgttatcacc ctctccactt tccgtaacgc tgaattcctg
     6181 caagcctacg gtgtggcaat tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt
     6241 gtggttattg acgaaaatga caatgtgatt ttcagccagc tggtggatga aatcaccacc
     6301 gagccggatt acgaagcagc tctggctgta ctgaaagcat aatttaatat gcctgatggt
     6361 gttgcaccat caggcatatt cgcgcttact cttcgttctt cttctgactc agaccatatt
     6421 cccgcaactt attggcaatc gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg
     6481 tgctgggata attgcgataa agctgggtta ataccgagcg ttcaaaacgg ctggtgattt
     6541 cgtccagcga accttccatc gcatcttcgc ccacggctac cgttgcggcg tcataatccg
     6601 gcaacaaaat atcctgtgga cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga
     6661 tagcgttctt taactgccgc acatttcccg gccacgcata acgcgtaagt acagtattca
     6721 ggtcagcggc cagtttcgga cgcggcacgc cctgctcgtc ggcaaagcgg gcgacgaaca
     6781 gctcagttaa cggcatgatg tcctgcggac agtcacgtag cggcggcaga ttgagcgtca
     6841 acacgttcag acgataatag agatcttcac ggaacatgcc tttttgcacc agttcgacca
     6901 gattcttctg cgtagcgcaa atcacccgca catcgacatg cacctcatgg tcttcgccaa
     6961 cccgacggaa agtgccatca ttaaggaaac gcagtaattt cgcctgcatc cgtggtgaca
     7021 tttcccctat ttcatccaac agcaccgaac caccgttcgc ctgctcaaag aatcctttct
     7081 tcccttccgg agcatgacca aacagttcac tctcgaccgc atcttccggt atagacgcac
     7141 agttcagcgc caggtaaggt ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa
     7201 agagatcttt acctgtacct gtgtcacccg taatcagcag cggcgcgctt agcatcgcca
     7261 gtttctgcgc ctgttcgaca acatgcttca ttttcgggct gacggcgaca atttgactga
     7321 aggcgctgac gtcctgggcg gcgacatttt gcaactggcg gcccatacga atcgttgatc
     7381 gcaacatcac caccgcaccg gtcaggacgt gttgatcatt ttcatcctga agataaacag
     7441 gcgtaatctc catcaggaaa ttctgcccat taataacgac atgctcgtta tgggaatctt
     7501 gcggttcgct ttccagccaa cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat
     7561 ggttgcgcag gcgatccaat ttttgcgcaa aaagctgaca gctcgccggg ttcgccatat
     7621 ccactttgct tttcatatcg acagagagca caggttcggg caacgcctcc agtaacgcgc
     7681 tcaacgccag atgctcacgt tcggaaggca tccacgggac agtacgcaca tcggtaacac
     7741 ctgcaatacg gcgtatttcg gccatcagac tgctgaaact ctcaaactcc agttcagcaa
     7801 aattgaggta gattcgccca atgggatcaa tctcaatacc gcgtaaatca atgcctctta
     7861 gcacgagtag atcgagtaat tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca
     7921 gacgcatggg aaccttcacc tgaaaaaaga acaattaata tgatgatatg acactatttg
     7981 gatagcagga aggtttctgt caacaatcat tgacacaaag acggaaaaac gggcacttcg
     8041 gcgtaaagat atccggtcag cacccgatat tgcattattt ttcgctgggc gttttgcctt
     8101 tttgcagcgt ttctttcacc tgaccgataa gctgacgacg gaaatccccg aggcgaggtt
     8161 tgtcatcgtc aatccacggc agcgggcggc aaagctccat agctttaatc ccgagtcgtg
     8221 ccgtcagaag tccagcacca attccctgag ctgcgcgggt agacaaacga gcagcgagat
     8281 cttgcgacat ccagtccatc cccacttcgc gcaccagttc gctggctccg gcaaaagcga
     8341 tattcagcaa taccagctta aacagacgca aacggctgta ataccccagt tcaatgccat
     8401 acagcgtggc gatgcgatta attaaacgca gattgcgcca ggcgataaac gccatatcga
     8461 ccaacgccag cgggctgacc gcaatcatca acgttgattc cgccgccgaa cggctgattt
     8521 cgcgccgcgc ctgggcatct aaaactggct ggaccaaatg cgcatacaaa ctgaccactt
     8581 cacggtcgtt ttgcgtttca tggattgagg cataccagcg ttgcagcgct ggatgagact
     8641 gatcaatacc cgcctgctgc gccagttttt cgcaaaatgc gcggcctttg cccgtgccgt
     8701 ggctgtgcaa caaatcgcgc gcttcgtcgc gttcatgggc gcgctgtcgc aagcgccata
     8761 agcgccgcca ctctgttacc acagaaccta cgccagcgcc gataatcaat gccccagcgg
     8821 cacatccacc cagcgccacc cagtcctgag tttgccaggc attcattgtc cactgtacac
     8881 cctgcccgac aacgcttgcg ccaaacagag ccagcccgcc catcaccatt ttgcgccaca
     8941 ggctgcgttt cggacgtaac gctgcatcca ttaccgcttc aacttgcccc tcttcttcag
     9001 gcgcttcatc gagcgtggcc ggggcaaaat tttgcgcctg attttcgtca aaggtctgct
     9061 gcgccctgaa ttttggattc tgatcgacct ccagcggacc gtcgaaatca atacgtggtt
     9121 ttaacggttc ggtcatcgca atttatctcc tattaaaaat tcaagcgcgg catcaagacg
     9181 aatatgcggc agcggtttgt cgacatccat cacctgcggg cgaaacgctt caaactggaa
     9241 cccttgcttg tcccagaacg cctgaccggg caaacgcgca ggaacttcac cgggataaac
     9301 ggtgagtggc gcaccatcgc tgagccggtt accgcgcaat gccggaattt tctcgccgtt
     9361 gacgtcaata atgccgctgg tggtcgcctg caccgaggcc agccccaggc aatccatact
     9421 aatcccttca aacgccgcat tttgccaggc atcctgaatc agttgttgca gtaatgaaac
     9481 catattggcg tgctgatcga tggtcacatg gtccgcttta gtggcagcaa acaatagctt
     9541 atcgataacc ggcgaaaaca aacgccggaa caaggtacgc tgcccgtagt ggaaactttg
     9601 catcagctgc gtcagtgcca gacgcatatc attaaatgcc tgtggcccac tgttgagagg
     9661 ttgcaggcaa tcaaccagca caatctggcg gtcaaagcgc agaaaatgat tcttatagaa
     9721 ccccttcacc accttctcgc agtaataatt aaaccgctcg cgcagcattc cggcattggt
     9781 gtgcttatcg gcctgcgcca gtttggactc gccccaggca tcgacatccg gccacgggaa
     9841 gaattgcagc gcgggcgcac ctgccatatc tcccggcaag acaaagcgcc caggctgaat
     9901 aaagtgcagc ccctgctgtt tacagtggtg gagataatcg gtccacgcgg cggcaatgtc
     9961 tgccagccgg ttttcgtcgg caggcgctag cgggtccagc ccttcgcaca tcattcgcca
    10021 tttgaccgac cattcgccgc gctgaccatt gagtaagccc gtcatctggc gtgaccagct
    10081 taaatagtcc tgcgccagca tcggcaggtc gagcaaccat tcgccgggat aatccacaat
    10141 ctccagatac agcgtggagg tgtccttgaa gtggcgtagc agcgaatcgt tcgatttaaa
    10201 acgtagcgcc aggcggattt cactgacgcc gcgcgttggc gtcggccagg cgggaggatc
    10261 gccatataac tgcgccagtc cttcatcata tgtgaagcgc ggaatgccaa agtcacgctg
    10321 aggaatgcgt tttacgccca gcaggcgctc ttcacgcacc gcgcttaaca gcggcaagcg
    10381 tgctccggcg tgaatattga gcaactgatt gaccatcgca gtgataaacg ctgttttgcc
    10441 gctgcggctt agtccggtta cggcgaggcg cagatgtctg tcgacacccc gattcaccag
    10501 cgcattaagt tcatttttaa gtcgcttcat tgctgtcctg tgctgctctg aagaccagaa
    10561 aaaaatcatt cgtaaggata tgaatgtggg ggccaggcca ttgattatca actgcccgtg
    10621 ttgtcatcga cgggcgaact ccataagatg tgcagcattc acgttcggac ggagttcctg
    10681 acctatgtcc cctactattt atgatattgc cagggttgca ggcgtatcaa aatccaccgt
    10741 atcacgcgtg ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa aagtgttacg
    10801 ggccattgaa gaattacagt atcagccaaa caagctggcc cgcgcgctga cctcttcggg
    10861 ttttgatgcc attatggtga tttctacccg ttcgaccaaa actacggcgg gtaatccgtt
    10921 tttctctgaa gttttgcatg ccatcaccgc caaagctgaa gaggaaggtt tcgacgtgat
    10981 attgcagacg tcgcacaatc cggcagaaga cttacaaaaa tgtgaaagca aaattaagca
    11041 gaaaatgatt aaaggcatta ttatgctaag ttcgccagcg gatgagtcat tttttgccca
    11101 acttgataaa tacgatattc ctgtcgtggt gattggcaaa gttgaaggtc aatatgccca
    11161 tgtttattct gtcgataccg ataattttgg cgacagcatt gcgttgaccg atgcattaat
    11221 tgaaagtggg catcaaaata ttgcctgcct gcatgctccg cttgatgtcc atgtttcagt
    11281 ggatcgggta aatggttata agcagagcct ggctgcgcat aatattgcag tgcgtgatga
    11341 atggattgtt gacggcggtt atacccatga aacagcactg caagccgcgc gggaattatt
    11401 aagccagtca ccgttgcctg aggcagtgtt tgctactgac agcctgaaat taatgagtat
    11461 ttatcgtgcg gcagcagaga aaaatattgc tattccgcag cagttagcgg tggtgggtta
    11521 tagtaatgaa acgctgtcgt ttattttaac gcctgcgcca ggcggtattg atatcccaac
    11581 acaggagtta ggtcaacaaa gctgtgagtt attattccgc ttaatttctg gaaaaccgtc
    11641 accacagaat attaccgttg ccacgcatat gacgctgaaa taaataccag ctaccttgcc
    11701 tgaatgccgg atgcgatgct agcgcatctt atccggccta catttatagt tcggataaag
    11761 tactcgtgcc gcatccggca atttagccca ttatcagaac gagtaattta cgccgacacc
    11821 tgcataatgg aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa acgcgtattc
    11881 cagcgaaacg gaaagcccgt tctggaaatc ataaccgtaa aataaaccta cacggttaaa
    11941 atcatggcct tcacgttcaa tatcatcctg ccagtcccag ttactccagc gatccagccc
    12001 aatgcgcgta tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat aggcgcgaat
    12061 ttcttgcgta gaaaactcac cgttattgcg gctgtcgtcc atattaaagc cgcgctcgag
    12121 ataatagttc actcgcaagg caaccgtttc gttgaaggta tattgcaggc ctgtttccgt
    12181 ttcgactcgg gtatcagcgt aaccggtcgt gttcagatcg ttggcaaatt tatacatcga
    12241 caaccaaccg ttgaaacgta aatcgtcagt cagtttcaca tcccagtctg gcgcgatttt
    12301 ccagcgctgc atattcgcag tgtctttacc cggttcatca acgtagtgat aaccataatt
    12361 acggaaaccg ccggtcaggc cgaaactgaa atcatcgttt tcgaggaact gataatgcac
    12421 ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat ctaccggccc
    12481 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
    12541 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
    12601 cgcgccgata ttaaagtgcc agtcgttcct ttcctcggcc tgtgcgcagg ccattcccgc
    12661 acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt gtattattct
    12721 gggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc atcaaagaaa
    12781 tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata atcatttaac
    12841 gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca
    12901 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
    12961 tgaatatctt ccggacgtat tcccatcacg atgggcttat gcaaacttcc ctgtgttttc
    13021 agaaccgcta atttctcttc gggaatggtt aatttaagcg tttccgtaac gaatttatcg
    13081 ccatcgatcg tgccacgaat aaaattcatc gccggtgatc caataaatcc ggcaacaaac
    13141 atattcgctg gttggttata aaccgttttc ggcgcaccaa cttgctgaac aatcccatcc
    13201 ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
    13261 atcatggtgg tattcagctt ctggtgtagc ttgctgatct ctgcgcgcat ttgcacgcgc
    13321 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
    13381 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
    13441 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
    13501 tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc
    13561 ggatacaacg cgtagttctg gaacaccatc gctatattgc gtgctttggc tggaacgtca
    13621 ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc
    13681 atgcgcaggg tggtcgactt accgcagccc gacgggccga caaacacgat gaactcttta
    13741 tcggcaattt ccaggttgaa ctccttcacc acatgcacct ggttatcgta gattttttga
    13801 atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc
    13861 cgataaccgc gaccaggtca gagagtccgt tgaaggcaac agtaattgcg ccccggttaa
    13921 gcccgcgccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg
    13981 cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcggcgag
    14041 aaagatttcc gggtccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa
    14101 aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cagaagccag
    14161 cccaaccggg atctgctgtg cacggagatc tgccagcaaa gagcgaatgc cgggtagaac
    14221 agcgctgacc gtcaactcgc gcagtgagtg gacatagagc agatttttgc gatacgccag
    14281 ttgcgccctc tcctgcgagt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg
    14341 cagagactca tcgcggctga tcccttttag ggattcgtta aactgcgcat caatgctgat
    14401 gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat
    14461 tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgtgtggta
    14521 gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg
    14581 gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc
    14641 tgcgcgtcaa gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt
    14701 tgccactgct caggtaacgc cggattgaga tgcaattcac cgtcacgcac gctcaccccg
    14761 gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca
    14821 tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa
    14881 ctttgggtca gcaggccaca gcgtgcggca acaatgccgt ggattgcttt acttaatgac
    14941 gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgctgag
    15001 aactgctccg gcagcatgta attgagcatc accacatcag cttgtttgag gatttgcatc
    15061 tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg
    15121 tatttcgcca gattaatcgc cggcttagcc ataaacgaat catcctgcgg caaaacgccg
    15181 tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcgcg atggataaac
    15241 gcatcgtcgc tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac
    15301 gccatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc aggcccaata
    15361 acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca
    15421 gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc
    15481 cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag
    15541 gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct
    15601 tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct
    15661 ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc
    15721 ggatcgctaa acagatgaaa cggtaacaaa aatacttctg tatcccagaa aacgtggcct
    15781 ttgtagcctt cgccggttaa gccttttgcc gcaatgctgc tgcgctcgtc gtgggcaggc
    15841 gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcttcgccg
    15901 ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt tgatactgca
    15961 agaagttggt cgtaactctg ctgcgcgcac atttcaagct ggcgaagcga cgcgctgccc
    16021 cactcgtcta aaacggcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac
    16081 gtcactgtct cgcctgcatg aagctgcgca ctggtatgtt gctgcaaacg gcgctctttg
    16141 gcggtatagc attgctgcac atcaccgctc accatacagc aacagctgat ggccacatca
    16201 ctgcgtccat cctgggtggt gtagatcccc tgcatcagat gctgaccaaa cacccgcacc
    16261 tgggtttcgt cgagatgttg gcgaccgtgg ttggtttgcg tggcgtcgat acctgttgaa
    16321 atcagcactg acgcgtcggc gtccagtggc gtaatagtga tttccagcac aatgagcggc
    16381 agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgtgcc gttgctggta
    16441 cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct
    16501 tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc
    16561 aggttcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac
    16621 atccctcgcg tctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg
    16681 ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct
    16741 gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgataca
    16801 acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct
    16861 tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca
    16921 gttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa
    16981 acagcatcgc accggcttta tcaccacaaa agctgacgtt cataatcgac tgttcgcgga
    17041 tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc
    17101 caaataacga atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccgcagc
    17161 tcttttgcgt gccgatcttt tgaaagctat gcgcattcac gcttttcacc gccggaaagc
    17221 ccagcacata catcgccgca tccagcatat gaatgccgat gtcgataagc gggccaccac
    17281 cctgcagttc tttattggta aagacacccc agccgggaac gccgcagcga cgcagggcgc
    17341 gggcggtggt aacgtaaatc tctcccaaaa cgccgttggt cacctgttca cgcagctgtt
    17401 gcgtatcgag cgcaaaacgg tggtgaaagt cgtaggccag caccttgccc agtttgcgcg
    17461 cggtatcgca catttcccgc gcctgttctg gcgtcatggc gggcggtttt tcgcacatca
    17521 catggcagcc cgcttccagt gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc
    17581 agacgctaac cacatcaggt ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg
    17641 atgcattacc gtatttatct gccagcgcct gcgcctggga aaggcggctg tcacagacag
    17701 ccaccagttc cagatcgttg cgggtgcagt agtacgaagc atgaacctta tccgccacct
    17761 ggcctgcgcc tattatcgcg acccgcagcg gagagcttgt cattgcactt ttcacaaaga
    17821 cctcttagca ggtacgcaac caggccaacg aatcacggta cgcctgggca ggatcttccg
    17881 cccggatacg cccttcgtac actacatagc cctgatagtt atccgcgcgc agctgttcaa
    17941 acagcgcgtg gaaatccagg gtgccgctgc ccggctggta gcgatggtta tccgcaatat
    18001 gcacatgacc gagcaggtcg cggttgtcat gcagcgcctg cgccaggtta tcttcttcga
    18061 tattcatgtg atagaaatcg ccgataatct gtacatgttt aagatcgttt tcgacgatgt
    18121 aacggcgggc atcggcgagg gtgttgatca tatgatcctg atagcggttt aacggttcga
    18181 gatacaccac ggttccggta cgcgcggcga cctgttccag tacgtgcagg gaatcactca
    18241 ccattttgcg gtcgccgtcc aggctacgcg gcgaggtcat cggcggtaag cggaaggtaa
    18301 acatgcccca cgctgccgga acgacgatac ctttaccccc cacttctgcc agcgcttcga
    18361 gaatgcgttc gatctgcttt aagccattaa gacgacgctc ttcgataaag tcgccaatcc
    18421 aaccgtcata gccaccgcag gcggtggtca ccggtaaacc ggtttctttg attgctgctt
    18481 tgacttcttc gagattgttg accagcagtt tgccgtcaat ctcaaaacca tcgaagccca
    18541 tctctttgat ataacgaaat ttctccagaa tgttttccgg aaagaatgcc tgattctgtg
    18601 tgcctatttt cattttttca ttcctttgaa ttaaaacgta acgcccattt tgatgctctg
    18661 ttccggatgc tgatcgacat actgcatgta gctttccggg ctcttggcaa aggtcactac
    18721 cgggtcgatt aaatcttcgc aattgagata accgttcatc agcagttccc agcaggtttc
    18781 ttcaatacgc ttacggctcc agcgcggata atccgggttc ggttcgctgc acgcacggga
    18841 gaaaacaatt ttggcgttat tgaaatgcgc ttcgcgtccg aggttaaaac cttcagcaaa
    18901 tggtttggca aacgcgacat aagagatggt gccgccataa gcaaggccac gcagcgccga
    18961 ttgcagcgcg tcggcgtaac cgctggtttc gataatcaca tcggcaccct gcttgccggt
    19021 cagcgttttg atctcttttc ctacgtcagt gccaatgggg ttaaggcaga aatcagcacc
    19081 gtggcgacgg gcaatatcac agcgataggc gataggatcg acgccaatca ccaccgacgc
    19141 gccagcgcgt ttcgccagtt ggatggctat ttgaccaatc gcgccaagcc ctaccaccac
    19201 cacaaaatca cccacgcgca cgttggcatc gcgcacgccg ctcatggcaa actgcgccgg
    19261 gtcgtaacaa acggcgtttt tccacgagct gccttccggc attttgcgca gcttgtagtt
    19321 attcaccgcg ttaatgatga ccgtctcgga gagcgggccg tagccgcata cgctgtcgcc
    19381 caccgcgtag tcggtaacgt cgctgccgca ctcgataatg tcgccaacca ccatgttgcc
    19441 aagctggaat ttgccaaact caatgccgcg cggcgcatct gccggacgcg gagtgaacat
    19501 ctgccattcg ccgttaaagt cttcatcgat aaacgggctg gcggcgcgga agtcgaccac
    19561 ttccgttccg tgtttcggtg cgccgaaacg cacgcggatt ttcacttcat tggctaaaat
    19621 cgcccgatct tcatactcaa ccagcgcagc aacacgcggt gctgtggcta ctaacttttt
    19681 catttaaaac tccttgtaaa gaaactggcc gctttagccc ttcacgccac cggcggtcaa
    19741 accactctta ataaaacgtt ccgaaagcgc atacataatc accaccggca atgcggtcac
    19801 cagtgaggcc gccatcatcc gtccccagat gtaatctggc gtactgaaca gcgcgttcag
    19861 gcccaccggt aaggtgaaat tgctggcgct ggagaggaaa atcgacgcaa acagatagtc
    19921 gttccacgcc accataaagc agtagacgaa cacggatatc agcccggaca tcgccaacgg
    19981 cacggtgatg cggaagataa tttgcagacg gttgaggcca tccatcatcg ccgcttcttc
    20041 gatctcatcg gggatggtgt cgaagtagct tttcagcata aacacggcgg tgggcagagt
    20101 ttgcgtcacc atggtgatga tcagcgccat ctcggtgtcg taaatgccca gcgcggtgat
    20161 gattttgaac agcggcacca ccagcaaaat gccagagaac atatacaccg tgtaaaagct
    20221 ggcattgatg gtcatccgac ctttaaagcg caggcgggaa agcgcgtaag ccccaagaat
    20281 gccgaggaat accgccacca cggatgaaac caccgacacc accagactgt tacggaagta
    20341 gtcgacaaac gggaaaatca tcgggttaaa aatgtcgacg taatgctcca gcgtccactg
    20401 ctgcggcagc agcgtaggat gcagtgagat cgcctctttc gcgcccttga acgaggtcat
    20461 cagcatcaca aaaaatggaa acagcgtgat gatgagaaac agcgccagcc cgcagtaaaa
    20521 accgatgcga ctgagtgtgc gtttatttgt tgccattgag gttcacccgt ttcctggtca
    20581 gcagaatgac agcgaaaata atgatgaaga gcaccaccga gatcgccgcc gctttcccta
    20641 agtcattaaa ggcaaaggcg gttttgtaga gatatacacc gagaatatcg accttggtcg
    20701 tcagcaaata aacatccgcg aacatgtaga acatccagat ggtgcgcagg gtcaccaccg
    20761 tcgccaggac cggcataatc gcgggcagcg tgacgatacg aaaccgttgc caggcattag
    20821 cgccatccat ctctgcagct tcgtacagcg atttgtcgat ggtctgcaaa atcgccagaa
    20881 acgagataaa ggcatacggg aagtagcgcc agatggcgaa cagcaccacc agcacaaagc
    20941 tactgcccgg attatcgaac cacagcggtg cctgctcata gagatgcaga agatcgacgc
    21001 cgaggtagtt cacaatgccg tagccgttgt tgaacatgta tttccaggca aacaccagcg
    21061 aaatggacgg cgttacgtag gagaggatca ccagcgaacg cgcggttttg cgcagatgga
    21121 actcgcggtt gaaaaacatc gccactgcca gcccgagcac ggtgctgccc gccaccacca
    21181 gcgcggtata ccagaccgtc atccacagcg agtgccagaa gccgggatcg gagaggatac
    21241 gcacatagtt actcacccca acaaaggttg actcgatgtt gggattgagc ggcagacgta
    21301 aaaaactgat ttcgatattc gacaccattg gccaggccac cagaccaccc agcagtaata
    21361 agctgggcgc gagaagcagc agcgcaaagg gcatatcgga acgacctgaa aaaagcctgt
    21421 tcattgcgga ttcctgtcct gggtttagtg ctgttcgatc agttcatcca gctttttctg
    21481 gctcgcttgc agcgtgccag ggagatcggc tttacccacg gtgacgttat gcaccatgga
    21541 gctcaccact ccagaccccg tcacatcacc catgcgggta aagtttttat cccctactgc
    21601 accaaagacc tgaatatttg gcagttcacc gattagctga ttcggtagtt cacccagcgc
    21661 cttaataacg tcgttgtctt tccaggtggc ggtagtgaca accgctttat tcaccggcag
    21721 cgcagcacct ggcgacatca tcacccagtc ggcaatgttg tctgcctgct ccataaaggt
    21781 gacaaatttt tctgctgctt ccgtctcttc ggtcttttgc ccggcggtaa tggtcagcga
    21841 ggtcaacatg ccgtagaccg cagagttttt ctcggttggc accacgaaac cgacgttttt
    21901 cgggtcgcct tctttaatca cagccagaag gatataggtg gagtaaatcg ccatcggcgc
    21961 ggtgccgttc ataaaggcgt ctttcacttc catgatgtcg ttagaacccg gcatagtgtt
    22021 ggcagcaagg tcgcggtaat aggtcagtgc ctgcatcatc tctggtgtat caagggtgat
    22081 tttgccttcg gcgttaaaga cgttagcctg gttggataac gcaaactggg agaaggattg
    22141 ttccgtcaac acgctttctg ctgtaggcag cgcaatgccg tattttttat tcgccgggtc
    22201 attcagtttc tgtgcaacgt ccagcagctg ttgccagttt ttcggctcct caagccccgc
    22261 ttttgccagc acatctttgc gataccagat accgccaatc caggcgctga caggaacacc
    22321 ggtccatgcg ctaccatctt cggtacgcac aatacgcagt acgccatcgt aaaacgcgcc
    22381 ttcaccaacg ttactgatga ctgtggcaac cgctttgcga tcgataagct gctctttgtc
    22441 catcactttg gcgtagtcat ggctggtttc gatcacttcc ggcagcgaac cgctacgtga
    22501 aagagtaatg actttagtgt tataggcatc ttcttccacg ggcacttgct taacggtgat
    22561 gccagggttt tctttttcaa aacgggcaat caatttactg ataacggcct ggcgctcctg
    22621 ctccaccgaa gaatgcataa attcgatgga tacattcgtt ttattttctt ctttacagcc
    22681 tgaaatcagg gcgcaggaaa ccagtgctga taacagcacg atttttgatt taatcatgag
    22741 atgttccttt ttatttaatc cacataacct gccaagggcg cagcgttatt tcattaccca
    22801 atatatcaac ttcactaatc agatcccgac catgcagatt agtaatattt acatgctgaa
    22861 tatttttact gacattaaac aatccagtca gacaattacc atcagcgtta cttctttgaa
    22921 tacgcattac ggatgaatta atcgtatcga tggtaaaatt attatccgga tgaaactcgt
    22981 tatggctgcg acgaagtgta attaaacgcg acaactcatg atataccgca tgccttaatg
    23041 tagcttcatc gttcagttct cgggttatct ctttactgtg atatttttta cggttaatcg
    23101 cacggttata tccgagtttt tcgacacctg cgtaatcatt acgcgagcca agaatacttt
    23161 gaatatatat cgctggcaca ccggggaaac ttaacaaaat cgcatgggcg agaataaacc
    23221 tggcgcaacg ttcttcatcg ctactctcac ggcggcttaa cgcatccata taggtcacat
    23281 ttatttcata cggactgcgt gtaccgtcgg gattattttt ccagtttact aatgcacctt
    23341 cctgctgtaa cgcctcgacc agctctaata tttcgctttc aggcaacaag ccccgtagcg
    23401 ggtttagccc gatgccatcg tgagaggcga ggaagttaaa ccaggtggtg ttgctggaag
    23461 gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac gttctgtttt tgcaccgcat
    23521 gcagcaccag tggcggcagc gagaactggt acaccatatg tgcttcgtca tcgcctgcgc
    23581 caaagtaagc aatgttgtct ttatgcggaa cattggtctc ggtaatgatc actgtacctg
    23641 gcgcaacgtt atcaataatc gaccgtaaca gtttgataat cagatgtgtt ttttccagat
    23701 ggatgcagct ttttcccggc tctttccaca taaagccaac ggcatccagg cggacatact
    23761 cagcaccttt cgcaaggtaa cagagtaaaa catccaccat cgccagcaac acttcagggc
    23821 tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg cgcgttgaat
    23881 gatcgcgcat ctggaatggc gttaataacg gtaacgcacg cgggcgagtg acggcgctga
    23941 gatcggtttg cgggtcaacg gcaataaaaa aatcttcaaa acctggatgc tgttgtaaat
    24001 agtttttaaa ccattcactt tttgccgaca tatggttgca gacaaaatca aacattaaat
    24061 gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg gcgacctgat
    24121 gataatcaat taccgaaaag ccatcatcag atgaccatgg ataaaacggc aacaaatgaa
    24181 catgtgaaaa aatgctttgc agccattgat ggtaaaactg attaaatgtg ggtaatggtt
    24241 ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa cacgacatca ctttcatccc
    24301 agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc gttacaagtt
    24361 tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa tccgtaattt
    24421 tctgtttcat gttgcctttc cgtggtagcg gtcccatgaa acgagagact actccgcaga
    24481 aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt ttttaccagg
    24541 gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg gatgtaaata aggtgtgaca
    24601 gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt gaccttcggc
    24661 attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat ctcgctaagg
    24721 atctcttttg cttgccctga ctggcgtccg gcattgcaat acactttcac ggtgtcgttt
    24781 ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat attgatggcc
    24841 ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca gtgttcagcg
    24901 gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt aaacatgtaa
    24961 gcgtccttct atggatgaaa attgccatcc attatacgcc tgagatttaa caatttttgc
    25021 gaaactcccc tcacctttta taacgctgtg ccagtttatt agcagcccga cttaacagcg
    25081 gttccagcgc cacagccagc aacattttca gcggtcggcg agcaactgat tttatcgccc
    25141 agcccgccac acccgccggg ccatagcgca gtgcggtaag aagtaccagc ttgcctgcta
    25201 atttgaaacc aggctttacc ttttgcccgg cctgttgcca gcgagtattc ataactttcc
    25261 tcacagttga cggaaacggc tacgtaacgt gaaagtatcg gaagtgacat aacgttccat
    25321 ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg acttcatcca gcaattcgct
    25381 gctggaaggt agctgctcac caaaggccat gttgtccggc attggatcaa gcgcaaatga
    25441 caaaatgatg taagcaacca gggtaaacag cgccagaccg aagaaaatcg acagcaccac
    25501 caggatacgc accagttttg ccggtacatc aaaatagttg gcaatcccgg cgcagacgcc
    25561 gcggaccatg ccctgctgtg gaatacgcca taatttttta ttgagattaa tgcccgccat
    25621 tagcgatccc tccagttcgg atgttctgca tcaagaatag attccagcgc ctgaatacgt
    25681 tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc gctgctgctc actttgcgac
    25741 aattcactgc gaccagaacg attgctgtaa tgcagccata accagatcgg taaaacaaac
    25801 agcacaaaaa tggttaacgg aatagccaga aatagcgcgc tcataagtac tccttagacg
    25861 atgagcggcg gcgcgttcgg acgccgcctg gatgttatta ttgattgtct tgcttcattt
    25921 tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc ggctttcagt tcggcaaact
    25981 gatcgtccag cgatttttgt ttaccgaagc tgtggctttc tgcttccgct tccatctggt
    26041 caatacgacg ttcgaaagat tcgaaacgag ccattgcttc atccagtttg ccactgtcca
    26101 gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg acgtaacatc aatgcctgct
    26161 ggcgagcgcg tgtttcgctc aatttgtttt ccagctcacc gatctctttc ttcatgcgtg
    26221 ccagcgtgtc gtccaccagc gtcacttcat gttccaggct cttaatcaga tcggtcagtt
    26281 tctgtttttc aattaacgct gcacgtgcca gatcctctct ctctttcagc agcgccagtt
    26341 cggctttttc ctgccattca acttcacgcg ccgacgcttg ttcaatacgg cgagtcagct
    26401 gtttcttttc tgccaacgcg cgcgccgaag tagaacgtac ttcaaccagt gtatcttcca
    26461 tctcctggat catcagacga accagtttct gtggatcttc cgctttctct aacagagcgt
    26521 tgatgttggc attcacgatg tcggcaaagc gagaaaaaat acccataatg ttgtcctctt
    26581 gatttctgcg ttcaggattg tcctgctgaa ctgttaatac aatttgcgtg ccaatttttt
    26641 atctttttga tttataaaga tctgattgaa gaatcaacag caacatgcca ggatgagtta
    26701 gcgaattaca ctaacaagtg gcgaatttca tcatggcaga atacaaagat aatttacttg
    26761 gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc gcatctcgca ccgctggaca
    26821 aaccggtgct catcatcggc gaacgcggca ccggtaaaga gctgattgcc agccgcctgc
    26881 attatctctc ctcccgttgg caagggccgt ttattttcct taactgcgcg gcgttaaatg
    26941 aaaatctgct ggattccgaa ctgtttggtc acgaagcggg ggcgtttacc ggtgcgcaaa
    27001 aacgtcatcc tgggagattt gaacgtgccg acggcggtac gctatttctt gatgaactcg
    27061 ctacggcacc gatgatggtg caggagaaat tattgcgcgt gattgagtac ggtgaactgg
    27121 agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg gttggtatgc gcgacgaatg
    27181 ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc tgacctgctc gaccgactgg
    27241 cttttgatgt tgtacaactg ccaccactgc gcgagcgcga aagcgacata atgttgatgg
    27301 cagaacactt tgccatccag atgtgtcggg aaatcaagct gcctctgttc ccggggttta
    27361 cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc gggaaatatt cgtgaattga
    27421 aaaacgtggt ggaacgttca gtgtatcgcc acggcaccag cgattatccg cttgatgaca
    27481 tcattattga tccctttaaa cggcgtccgc ctgaagacgc tatcgccgtt tcagaaacca
    27541 cctcgcttcc aacactgccg ctggatttac gtgagtttca gatgcagcag gaaaaagagt
    27601 tgctgcaact cagtttgcaa caagggaaat ataaccagaa gcgcgcggct gaattactgg
    27661 ggttaactta tcatcagttc cgcgcgttgt tgaaaaagca ccagatttag cgaagttgag
    27721 caatacaact tgcaggatgc ggcgtgaatg ctttattcgg catacataat caatacgtcg
    27781 tttgcaggcc tgataagcgc agcgcatcag acattattgc gttgggctat taatcgctca
    27841 gcgcatcctg caaaattttc attgccgcat cgaattgcac atccgggatg gtcagcggat
    27901 acaggaagcg aatcacgttg ccgtatgcgc cacaggtcag caggagcagc ccctgcgcca
    27961 gcgcgcgttg ctggattttc tgtgcaatcg ccgctgacgg cttgcccgtt tgcggatcgt
    28021 taaactctgc cgcaatcatc gaccccaggc cgcgtaccgc agcaatggcc ggaacgcttt
    28081 ctttggcatc aatcaacgtg tttttgagac gctggcccag ttgattcgcg cgttcgcaga
    28141 gtgattcttt gtcgataata ttgagcaccg cgtgcgcggc agccaccgcc agcgggttcc
    28201 cggcgtaggt gccacccaac ccgcccggcg cgggcgcgtc cataatattc gcgttaccga
    28261 ccacgcccga aagcggcatc ccgcccgcga ggcttttcgc catcgtcatt aagtccggct
    28321 tatcggcgta atgatccatg gcaaacagtt taccggtacg cgcaaagccg ctttgcactt
    28381 catcggcaat catcacaata ccgtgctcgt cgcacaggcg gcgaatggcg gcaaccagct
    28441 cttttggcgc aacgttgaaa ccgccctcgc cctgcaccgg ttcgaaaata atggccgcga
    28501 cctgcttcgc ttcgatgtct gatttaaaca agcgttcgat ggcgtcgagg gagtcctgtg
    28561 ttgaaatgcc gtgtaaatct gacggataag gtacgtgata caccgaacca gggaacgggc
    28621 cgaagccgat tttgtacggc gcaacttttc cggtcagcgc catggtcata tatgtacgac
    28681 cgtggaagcc accgctaaac gcaatcacgc caggtcgtcc ggtatgggcg cgagcaattt
    28741 tcaccgcgtt ttccaccgct tccgcaccgg tagtgaagaa tgcggtttta gcctgcccgc
    28801 tcactggggc aagggcgtta attttctccg ccagcgtgac gtagctttcg tacggcacaa
    28861 tctgatacgc cgtgtgggta aactgttgca attgctgctc caccgccgcg accagatcag
    28921 ggtggcgatg cccggtattc agcaccgcaa tgcctgcggc gaaatcgatg tattcgttgc
    28981 cctctacgtc cttcagcgta gcgttttctg ccgactgggc gaagaagtta cacatcacgc
    29041 caaccccgcg tggggtagca gaaagacgac gctgatggaa ttcattgttg ctcatgtttc
    29101 acaccttttg actcttcact gttaatcgga aattaaacgc ccagacgatc gcgcaggctg
    29161 taataagccg cgcccatcgc ggtaaatggc acgcgcagcg tgcgcccgcc ggggaacggg
    29221 taatgcggca gattggcgaa ggcatcgaaa cgttcggcgt cgccgcgcag cagttcggca
    29281 atcagacgcc cggcaagatg ggtacaggtg acgccgtggc cgctgtagcc ctgcatgtaa
    29341 tagatgttgg tatcaaggcg accgaactgc ggcatgcgcg acaaggtcag caggaagttg
    29401 cccgtccagc ggtaatcaat tttcacgccc ttcagctgcg ggaaggtttt cagcagtttc
    29461 ggcaccacaa ggcgctcaac gtcatccggg tcgcgcgcgc cgtagaccac gccgccgccg
    29521 tacagcaggc ggttgtcggc ggtaagacgg tagtaatcaa gcagatagtt acagtcttcc
    29581 acacagtagt ttttcgggat cagcgaacgg gctaaatctt ccgacagccg ttcggtggtg
    29641 atcacctgcg tgccgcacgg catgctgcgt ttcgccagtt ccggctctac tttatcgccc
    29701 agatacgcat tcccggcgac aatcacatac ttcgccgtca cctgaccttt ggcagttcgc
    29761 acaacggctg gcgtggtgtg ctggatctgc gtcacggcag aaagttcata cacgcgcccg
    29821 ccgttgaggc ggatggcgtc cgcttcgcca atggcgaggt ttagcggatg aatatgccca
    29881 ccgctgtgat ccagcagcgc accggtatag cgatcgctgg ctacttcacg gcgaatggcg
    29941 ttggcgtcca gcaattccag ctgtttattg ccgtagcgtt cccagttctc tttctgctct
    30001 tcaagtgtgg cgagctgttt atcattcatc gccacaaaca ggccgccggg gcggtagtcg
    30061 cagtcaatct gataacgttt gatacgttcg cggatgatct caccaccttc gaacatcatg
    30121 ctgccgagca tacgggcggt gtccatgccg tagctttttt cgatcacgtc gatgtcgcgg
    30181 ctgtaggagt tcacaagctg cccgccattg cgcccgcttg cgccaaagcc gatgcgtgag
    30241 gcttcgagaa ccactacgtc aaagcccgct tccgccagat gcagcgcgga agagagcccg
    30301 gtatagccgc cgccgaccac gcaaacgtcg caggaaatcg actcattcag cgtgtcgaat
    30361 ggcgcgtatt tattcgcact ggcggcgtag taactgcttg tatgttcggt catttcaggc
    30421 ctccaggctt atccagatgg ttttcagttc ggtgaatttt tcaagggcat gcagggattt
    30481 gtcgcgaccg ttgccgctct gcttatagcc gccaaacggc acggtcatat cgccgtcgtt
    30541 gtagttattg acgaagacgg aaccagcctt caggcggcgg ctcatgcggt gcgcgcggga
    30601 gaggtcgcgc gtccataccg ccgcgccaag gccgtactgg ctgtcgttgg caagctgtag
    30661 cgcctggtct tctgatgtga aacgcgtgac caccagcacc ggaccgaaaa tctcttcgcg
    30721 gcttaaggac gcattcgggt ctacatccac aaagatggtc gggccgatgg cggcagccag
    30781 ctcagcgtta cgaccatcca gcagcagttg ccctttgctt tcgccttccc gaataaagct
    30841 atggaccgag tcggcgtggg cgcagtcgat taaggtgccc atggtggttg cgggatcaag
    30901 tggatggccc ggctgccagt tttgcgcctg ctgttttaac agggctaaga attcatcggc
    30961 gatgctctct tccagcaaca aacgcgttcc ggcgatgcac acctggccct ggttgtagaa
    31021 gatgcctgcg gcggtggcgc ttgccgcttt ttgcagatcc ggacaatcgg cgaaaacgag
    31081 gttggcgctt ttgccgcccg cttccagcca gacgcgtttc atgttgctgt cgcccgcatc
    31141 tttcagcagc tgtttcccgg tacgggttga accggtaaag gcaatggcgt cgatatcgtt
    31201 atgacgcgac agcgcctgcc ccgcttcatg accaaaaccc gtcaccacgt tcaacacacc
    31261 atccggcaag cctgcttctt tcgccagccc cgcgagacga atcgcactga gcggtgattt
    31321 ttcagacggt tttagaatca cgctgtttcc cgccgccagc gccgggccga gtttccagca
    31381 agtcagcaac agcgggaagt tccacggcac gatggcggca atcacgccga ccggttcacg
    31441 cacgatcatc gccagctcat ggctactggt ggtcgccact tcgccataca ctttgtcgat
    31501 cgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga atatcatcac gcagactgtg
    31561 acgaatcggt ttgccggtgt cgagagtttc cagcagtgcc agctcttcgg cgtgggcttc
    31621 cattaaatcg gcgagtttat tcagtaccgc tttacgttta gccggagaag agagtgacca
    31681 gtcgccgcgt tcaaatacgc cgcgtgctgc gctcaccgca cggtcgatat cgacgctctt
    31741 gccgcgggca attttcgcca gcggtgcctg ggtgaccgga tcaacggttt caaaggtttc
    31801 attttccgcc gcagcagtat attcaccgtt aataaataag cggttttcaa tggcgagact
    31861 taacgcttta tcctgccagt aagccagatg atgaaaattc attatgactc ctgtttcacg
    31921 tctatcagat atatgcagtt tgtgaacgtc agggcgcaga gatccccggg ccgtatgccc
    31981 gtttatttct ggcgacattc tttatataca cttcatcctt caagccgcct ctgccttggc
    32041 tgcgttcgct cccccgaatc acttacctga gtaagctcat cgggattcgc tcacttgccg
    32101 ccttgatgca acttgaatga ttttgtgtat agattgccgg gcgcattgcg cttacccggc
    32161 ttacagaaca aaaagattaa aacgtggtgg gcgtatgggc gctgataatt cggcaaatac
    32221 ctgccgacgt attactgaaa ctgtgcggga tgccggtatt aatggcataa ctttgccccg
    32281 cgacgaggtg gtaatcctga ccattaatcg tcagaacaat ttcaccttcc agtactgtgc
    32341 ctatttcctc accctgatgc ttaattcttt ccccagtggt tgtgcccggc tggtacgttt
    32401 caaagatcat cgccagcgtg cgattcgggt taccattatg aaccagcttc attgacacac
    32461 cctgactgcc catctcaatt aagtcgtcct gattaatgac gacctgcggc tcatcaggtt
    32521 tttccggctc ggaaaagaat tccgagagtg acagaccata caccttcagc agcttttgca
    32581 gcgtactgat ggcagggctg actttatctt gttctatcgt actgatagca ctgtgagtca
    32641 gcccggagag ttcggcggca cgacgttgtg aaagcccctg ctgctggcgg atttccgaca
    32701 agcgttttcc tggcgccagt ccctcatcac tcatatttgc atttccttaa actgtagtgg
    32761 tcagagtcgc tgtttttcag cgatatggtg ctgacaagcg gtgataaagc cctcgaacaa
    32821 tatacgcgaa agcgcgtact cgctactgtt ccattccggg tgccactgta cgcccagcgc
    32881 aaaaggatga ttgatgacgc taaccgcctc aactaagcca tccggcgagc gagcttcaac
    32941 acgcaaccgt gggctaacga ccttcgcccc ttgcccatgc agagagttta cccaaaagtt
    33001 gctacattca ggtaacaacg cagacagtaa tcccccctct tcaacctgaa cttcatgcga
    33061 cggtgcatat tgctgttcca ccggcagttc gggatcttcc cgatgttcca gcaattcagg
    33121 ctgttcgcac agcttgcgat gcaacgaacc accggttgcc accaccagtt cctgtaaacc
    33181 ccggcagatg gcgaaaatgg ggatgcgcct ttcgagtgcg gcattaatta acgccatgct
    33241 cagaagatca cgcccgggat cggcgtcagg ctcatcgccg ttttcaccat ataggtgcgg
    33301 ctgcacattg ctgggactac caggaagata aatgccatcg agtttcggca aaagttgttc
    33361 aagtaatgac ggttccgcca gcgcatgtgg tagcgcaatt ggcaagccgc ctgcgtggat
    33421 gatggcattc aggtactttt cttgcagagt ctgggtcgca tgatccttaa gcctgttcct
    33481 gcacattacg acaccgataa ccggattgtt cattatattt tccatgctca atctcacaaa
    33541 gtggactaaa ttatcgccat tactgcggtt taaatgacct ttatgttcaa tattttttca
    33601 atctagcagt gggttttcat ttttgcaaac tcaatttaac atttgacaaa catttagttt
    33661 gcatacagat tcgaatggtg gtcattatat tttacgcttt gataacgagc ggaaaacaaa
    33721 ccaaaggcga agaatcatgg aaaccaatat cgttgaagta gagaactttg ttcagcagtc
    33781 agaagagagg cggggtagcg cctttacgca ggaagtgaaa cgctacctgg agcgctaccc
    33841 gaatacgcaa tatgttgatg ttttacttac tgatttgaat ggttgcttcc gtggaaaacg
    33901 tatccctgtt tcaagcctga agaagttgga gaaagggtgt tatttcccgg cctcggtgtt
    33961 tgcaatggat attctgggca acgtagtaga agaagcgggt ctgggtcagg aaatgggcga
    34021 gccggatcgt acttgtgttc ctgttctcgg ttccttaacc ccttctgccg ccgatccaga
    34081 gtttatcggt caaatgctcc tgaccatggt cgatgaagat ggcgctccct ttgacgttga
    34141 gccgcggaac gttcttaacc gcctgtggca gcagctacgc cagcgcggat tgttcccggt
    34201 tgtagcggta gagctggagt tctatttact ggatcgtcag cgcgacgctg aagggtatct
    34261 gcaaccgccc tgcgcgcccg gcaccgatga ccgcaataca caaagccagg tttactccgt
    34321 tgataacctc aaccacttcg ccgacgtgct caatgatatt gatgaactgg cgcagttaca
    34381 gttaattccc gccgacggtg cggtcgccga agcctcgccg ggtcagtttg aaatcaacct
    34441 ttaccatacc gataacgtgc tggaagcctg cgatgatgcg ctggcgctaa aacgcctggt
    34501 gcgtctgatg gcagaaaagc ataagatgca cgccactttt atggcgaagc cgtatgaaga
    34561 gcacgcgggc agcgggatgc atatccatat cagtatgcaa aataatcgtg gcgagaatgt
    34621 gctttctgac acagaaggcg aagattcgcc gctgctgaaa aagatgctcg ccgggatgat
    34681 tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac gtgaactcgt atcgccgctt
    34741 ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc cataacaacc gcaccgtcgc
    34801 cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg gaatatcgcg tggcgggtgc
    34861 cgatgccaac ccatatctgg tgatggcagc gatttttgcc ggtattttgc atggccttga
    34921 taacgagctg ccgttgcagg aagaagtcga aggcaacggg ctggaacagg aaggcttacc
    34981 cttcccgatt cgccagagcg atgccctagg tgagtttatc gagaatgatc acctgcgccg
    35041 ctatttaggc gaacggtttt gccatgtgta tcacgcctgt aaaaatgatg agctgttgca
    35101 gtttgagcgc ctcatcacag aaacagaaat tgagtggatg ttgaaaaacg cgtaattccc
    35161 catgcggggt tgtttcatcg gcctgagcgg agccaggcct gacatttgct ggtaatccag
    35221 acatttcccg gatgtcgcgt tatggagcgc aggcggcaac gggcttccgt taacgattaa
    35281 gactgtgacg aggaaatgag atgatgccga tgattaaatc cccgcatggc gagggaggct
    35341 gcgtatgcgc ccctcctgct actgactgga ctccgccccc tttgcttccc ttgctaaaca
    35401 ggtttgattt caggtcgaca cgaccgcaaa ccttattacg caggggaggc agcaattatg
    35461 gctattaatt caccactgaa tattgctgcg caacccggca aaacccgtct gcgaaaatca
    35521 ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc tcacgccgat gaccgtattt
    35581 gatacttttg gtattgtctc cggcattagc gacggtcacg ttccggcgtc ctatttgctg
    35641 gcgctggcgg gcgtgctgtt taccgctatc agctacggca aactggttcg ccagtttccg
    35701 gaggccggtt cggcctatac ctacgcgcaa aagtcgatta atccgcacgt cggatttatg
    35761 gtcggctggt catcactgct ggattatctc tttttgccaa tgatcaacgt tttgttggcg
    35821 aaaatctatc tctccgccct cttcccggaa gtgccgccgt gggtgtgggt ggtaaccttc
    35881 gtcgccattt taaccgccgc gaatctgaag agcgtcaacc tggtcgctaa cttcaatacc
    35941 ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta tcttcctggt ggttcaggga
    36001 ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc agccgtttat cagcgagaac
    36061 gcgcacctga tcccgattat taccggtgcg acgattgtct gtttctcgtt cctcggtttc
    36121 gatgcggtga ccacgctttc ggaagagacg ccggacgccg cacgcgtgat cccgaaagcc
    36181 atcttcctga cggcggtata cggcggcgtt atctttatcg cggcgtcgtt ctttatgcag
    36241 ctgttctttc ccgatatcag ccgctttaaa gacccggacg ccgcactgcc tgaaattgcg
    36301 ctgtacgtcg gcggcaagct ctttcagtcg attttcctct gcaccacgtt tgtgaacaca
    36361 ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc tgctgtatgt gatggggcgt
    36421 gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc caaaatggcg gactccggca
    36481 ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc tgttctttga tttagtcacc
    36541 gcgacagcac tgattaactt cggtgctctg gtggcgttta ccttcgtgaa tctgtcggtg
    36601 tttaatcatt tctggcggcg taaaggaatg aataaaagct ggaaggatca cttccactat
    36661 ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc tgtgggttaa cctagagtca
    36721 acgtcactga cactcggtct ggtatgggct tcgttaggcg gcgcatattt gtggtatctg
    36781 atccgccgct atcgcaaagt gccgctgtac gaaggtgaca gaacgccggt gagcgaaacg
    36841 taatagccgg atgcgcggcg cattatcctg cctgagggta acgcgccgcg ccattaacta
    36901 taaaaagatg tatgacccgg atcacgagtg ctatcatccg cccttatgct tagcgcatta
    36961 accccagggg caaataatga accacgatat cccgctaaaa tattttgata ttgccgatga
    37021 gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa cgcacaccgc tggcccacta
    37081 cttccagtta ctgctcaccc gtttgatgaa caatgaagag atcagcgaag aagcccaaca
    37141 tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt gatgagatcg cagagttttt
    37201 gaatcaatgg ggtaatgagt aattatttaa ttgcagacga tcggtcagta tcgttgttcg
    37261 gataaagcgt tcacgccgca tccggctaac aatgacacgc actgcagcgt gctttaatgg
    37321 gtttctcacg atcgtaccgc tctgctcctc gctaagttgt tctgtgagtt atagttatag
    37381 agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca cctgttcaac gaccaaccac
    37441 acggccctca ttacgacccc tgaacacaaa cggtaatggc aaatgtcatt aaatattagt
    37501 aagatagcct ctttacataa aatcccttaa tattatgcgc caggtattat cgtctctttt
    37561 ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg cctgaatccc ccccgcatgc
    37621 tgatatccgc gacagcggtt ttgtctattg cgtcagcggg caagtcaaca cctttaaccc
    37681 atccaaagcg agcagtgggt taattgtcga tacccttgcc gcccagtttt atgatcgact
    37741 gctggatgtc gatccctata cctatcgcct gatgccggaa cttgccgaaa gctgggaagt
    37801 actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc gatgttccgt ttcaaaaaac
    37861 cgactggttt actcccactc gtaaaatgaa tgccgacgat gtggtgttta ccttccagcg
    37921 aatttttgac cgcaacaacc cgtggcataa cgtcaacggc agcaacttcc cctacttcga
    37981 cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa ctggataatc ataccgttga
    38041 gttccgactc gcccagccgg atgcttcttt tttgtggcac ctcgcaaccc attatgcttc
    38101 ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa gatcgccagg agcaactcga
    38161 ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa taccgcgccg ggcaatttat
    38221 tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg ttaatgccgc aggtagtggt
    38281 ggatttaggc tccggcggca ccggacgtct gtcgaaactc ctgaccgggg aatgcgacgt
    38341 tctggcctgg cctgctgcca gccagctatc cattttgcgt gacgacccgc gcttgcgttt
    38401 aacgctgcgt cctgggatga acgtcgccta tctggcattt aacaccgcca aaccgccgct
    38461 aaataatccc gctgtccgcc atgcactggc actggcgatt aataaccagc gcctgatgca
    38521 atccatctat tatggtacgg ctgaaacggc ggcctctatt ttaccgcgcg cctcgtgggc
    38581 ctatgacaac gaggctaaaa ttactgaata caatccggcg aaatcgcgcg aacagttgaa
    38641 ggcgttgggg ctggaaaact taacgctgaa actgtgggtg cccacacgtt cgcaggcgtg
    38701 gaaccccagt ccactgaaaa ctgccgaact gattcaggcg gatatggcgc aggttggcgt
    38761 aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag gcgcggttga tggatatgag
    38821 ccatgatctg acgttatccg gttgggcgac ggacagtaac gacccggaca gtttcttccg
    38881 tcctttactg agctgcgcgg caattcattc acagaccaac ctcgcccact ggtgcgatcc
    38941 gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag cagctggcgg cgcgtattga
    39001 agcctatgac gaagcgcaga gtattctggc gcaggaattg cccattttgc cgctggcgtc
    39061 gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt ctggtactta gcccgtttgg
    39121 taacgcctcc tttgctgggg tgtatcgcga gaaacaggat gaggtgaaaa aaccatgatt
    39181 atcttcacct tacgccgcat tttgttattg attgtcacct tgttcctgct gaccttcgtt
    39241 ggcttcagct tgagctattt cacccctcat gcgccgttac aaggcgcgtc actgtggaat
    39301 gcctgggtgt tctggtttaa cggcctgatc cactgggatt ttggtgtttc cagcattaac
    39361 ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg cgacgatgga actgtgcatc
    39421 ctcgccttcg gttttgcgct gattgttggc atccccgtgg ggatgattgc cgggattacg
    39481 cgccataagt ggcaggataa cttgatcaac gccatcgcct tactgggctt ttcaatcccg
    39541 gtgttctggc tggcgcttct gttgacgctg ttttgttcac tcacgctagg ttggttgccc
    39601 gtttcagggc gtttcgatct gctctacgaa gtgaaaccga ttaccggttt tgcgttgatt
    39661 gatgcctggc tttcggactc accatggcgg gatgaaatga tcatgagcgc aatccgccat
    39721 atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa ctgaagtgat ccgcctgatg
    39781 cgtatcagca ctatcgaagt gtatgaccag aactatgtaa aagcggcggc gacccgcggt
    39841 ttgtcgcgct ttactatttt gcgtcgccac gttctgcata acgcgttacc gccggttatt
    39901 cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg cgatgatcac cgaaatggtt
    39961 tttagctggc caggtttagg gcgctggtta attaacgcca tccgccagca ggattacgca
    40021 gccatttccg ccggagtgat ggtgtgtggc tcactggtca ttattgttaa cgtgatttct
    40081 gatattttgg gtgccatggc taaccctctg aaacataagg aatggtatgc cttacgatag
    40141 cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc gcctggcgca aattttatag
    40201 tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg ctggctgtac tgtgtatttt
    40261 tggcggctgg tttgcgcctt acggcatcga ccagcaattt ctcggttatc aattattgcc
    40321 gccgtcatgg tcgcgctatg gcgaagtttc tttcttcctg gggactgacg acctcggacg
    40381 cgatgtttta agccgtttac tgagcggagc tgcgccaacc gtgggtggcg catttgtggt
    40441 gacgcttgcc gcgacgatct gtggcctggt actgggcacc ttcgccgggg cgacacacgg
    40501 cctccgctca gcggtgctta accatattct ggatacctta ctggcgatcc cttcgctgct
    40561 gttggccatt atcgttgtag cgtttgccgg accgagtttg tcccacgcta tgtttgctgt
    40621 ctggctggca ttgctgccgc gtatggtacg ttcgatttac agcatggtgc atgacgaact
    40681 ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca tcaacgctga atattctctg
    40741 gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc gagatcaccc gcgcactgtc
    40801 gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat ctcggcgcac aactcccctc
    40861 gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg atttatgtcg ccccgtggac
    40921 tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg ttggttaacc tccttggcga
    40981 tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc cattactcga tattcgtaac
    41041 ctgaccattg aatttaaaac cggtgatgag tgggttaaag ccgtcgaccg ggtaagtatg
    41101 accttaaccg aaggtgaaat ccgcggtctt gttggtgaat ccggttccgg caagagtttg
    41161 attgcgaaag cgatttgtgg ggtgaataaa gataactggc gtgttactgc tgaccgtatg
    41221 cgttttgatg atatcgattt gctgcgtctc tccgcacgcg aacggcgcaa actggttggc
    41281 cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc ttgacccttc agaacgtgtg
    41341 ggtcgccagt tgatgcaaaa catcccagcc tggacctata aaggccgttg gtggcagcgt
    41401 tttggctggc gcaaacgccg tgccattgaa ctgctgcacc gcgtggggat taaagatcac
    41461 aaagatgcga tgcgcagttt tccctatgag ttgaccgaag gtgaatgtca gaaagtgatg
    41521 atagccattg cactggcgaa tcaaccgcgt ctgctgattg ctgacgaacc gaccaactca
    41581 atggagccaa caacccaggc gcaaatcttt cgcctgctga cgcgtctcaa ccaaaacagc
    41641 aataccacta ttttgcttat cagccatgac ttacaaatgc ttagccaatg ggcggataaa
    41701 attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc cgagtaagga gttggtgacg
    41761 atgccacatc atccttatac ccaggcgctg atccgcgcga taccagactt cggcagcgcg
    41821 atgccgcata aaagtcgcct caatacgctg cccggcgcta tcccactgct ggaacagtta
    41881 ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac aacgagaatg cattgtgacg
    41941 ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc atttcccgct gaacatggag
    42001 aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat ttaagtaaaa cgttccgcta
    42061 ccggaccggc tggtttcgtc gtcagaccgt agaagcggta aaacccttga gctttacgct
    42121 acgtgaaggc cagacactgg cgattattgg cgagaatggt tcgggtaaat ccacgctggc
    42181 aaaaatgctg gcgggaatga tagagcctac cagcggcgag ttattgattg acgatcatcc
    42241 actgcatttt ggcgattatt ccttccgcag tcagcgtatt cgcatgattt ttcaggatcc
    42301 ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt ctggattttc cactgcgcct
    42361 gaacaccgac ctggaaccgg aacagcgtcg caaacagatt attgaaacga tgcgtatggt
    42421 tgggctgcta ccggatcacg tcagttacta tccgcatatg ttggcacccg gacaaaaaca
    42481 gcgtctgggt ctggcgcgcg cgctgatatt gcgcccaaaa gtcattattg ccgatgaagc
    42541 cctcgcttca ctggatatgt cgatgcgttc gcagttgatt aatctgatgc tggaattaca
    42601 ggaaaaacag ggcatttcgt atatttatgt tacccagcat atcggaatga tgaagcacat
    42661 tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc gagcgaggca gcaccgcgga
    42721 tgtgctggca tcgccgctgc atgaactcac caaacggctg attgccgggc actttggcga
    42781 agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt tctgaatcct ccttccagat
    42841 tatccacctc attctgacaa gagattaaat cttgtcagaa tgaggtgatg gataaaataa
    42901 aatcaaatgc gcgtgattta agacgcaatc tcactttgca ggaacgaaag ctctggcgat
    42961 atcttcgcag ccgacgtttt ggtgatttca aatttcgccg tcaacatccg gtggggagct
    43021 acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt tgagctggat ggtgggcagc
    43081 atgatttggc agttgcctat gataccaggc gcactagctg gcttgagtcg cagagctgga
    43141 ccgtgctgcg tttctggcat aacgagattg attgtaatga ggaggcggtg ctggagatta
    43201 ttctgcagga actgaaccgc cggtcaccct caccctgaaa gggcgagggg gccgaccgag
    43261 ccgaatagct gttgtggtga aaacatggag acagtgctgg agaatattcg gcaaggtctg
    43321 aaccatccca gccatcgcca taaaagggtt aggggctgta tgagcctgtt tgttgctggg
    43381 gtaacaatat ttgcacaata tggtcccctc gcccctctgg ggagagggtt agggtaaggg
    43441 gaaaagcgcc caccctgccg cagcctgctc cggtcggacc tggcaactat agctactcac
    43501 agccaggttg attataataa ccgtttatct gttcgtactg tttactaaaa cgacgaatcg
    43561 cctgattttc aggcacaaca agcatcaaca ataaggatta aagctatggg ttttctttcc
    43621 ggtaagcgca ttctggtaac cggtgttgcc agcaaactat ccatcgccta cggtatcgct
    43681 caggcgatgc accgcgaagg agctgaactg gcattcacct accagaacga caaactgaaa
    43741 ggccgcgtag aagaatttgc cgctcaattg ggttctgaca tcgttctgca gtgcgatgtt
    43801 gcagaagatg ccagcatcga caccatgttc gctgaactgg ggaaagtttg gccgaaattt
    43861 gacggtttcg tacactctat tggttttgca cctggcgatc agctggatgg tgactatgtt
    43921 aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca tcagctccta cagcttcgtt
    43981 gcaatggcaa aagcttgccg ctccatgctg aatccgggtt ctgccctgct gaccctttcc
    44041 taccttggcg ctgagcgcgc tatcccgaac tacaacgtta tgggtctggc aaaagcgtct
    44101 ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc cggaaggtgt gcgtgttaac
    44161 gccatctctg ctggtccgat ccgtactctg gcggcctccg gtatcaaaga cttccgcaaa
    44221 atgctggctc attgcgaagc cgttaccccg attcgccgta ccgttactat tgaagatgtg
    44281 ggtaactccg cggcattcct gtgctccgat ctctctgccg gtatctccgg tgaagtggtc
    44341 cacgttgacg gcggtttcag catcgctgca atgaacgaac tcgaattgaa ataatcattc
    44401 tgttggtaaa tattgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
    44461 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
    44521 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
    44581 gaaacgcaat ccagtactgc ggagtatgac gttctgcctc tggtcccgga agccgcaaag
    44641 gtcagcgatc cctttctgct cgacgtgatc cttgatgaag aaacgctggc tcccttcctt
    44701 tcatggctgg tccctgcgcg cgttcttgca gtggaattgt tccctgacca gcttaccgtg
    44761 acccgttcac agaccttcac cgcttatgaa cgcttgtcta cggccctgac ggttgctcag
    44821 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccggg
    44881 cctgattcct ccagggaaag caatcatagg ttggcacaaa tcacgcaata tgcccgccaa
    44941 ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
    45001 cttactgccg gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
    45061 cggaccattg cgacatttca ggcttatctc gggcacccgg tacgctggtt acccggtctg
    45121 gaaatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc
    45181 agctatgagg tggaaaaact acctgaagag tacacaaaaa gttcaacagc agaactttgc
    45241 caactggccg aaacactctc tctccaccct atttcacttt cccttctcga aaggttgtta
    45301 aacagtacac gggttaatac acagccggat aataagcttg cggcgttgtt atgcgcgcgg
    45361 ataaatggca gtcctgcttg ttttgccgcc tgtatggatt catcaaatga atataaaaaa
    45421 atcagccccc ttctgcgcaa gggcgaaaat gaaattaacc aatgggctga ccgtcattct
    45481 gttgagcacg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
    45541 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
    45601 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
    45661 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
    45721 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
    45781 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
    45841 ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
    45901 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
    45961 agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
    46021 tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc gcgcagcccg
    46081 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
    46141 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggtgacga
    46201 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag aagcaccaga
    46261 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga ccgcgctgga
    46321 ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt tcgctaaggc
    46381 gttgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
    46441 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
    46501 cttcaacatc cctatgctgc ctcgcgagct ttctgaggat ctctgctcac tgcgcgccaa
    46561 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
    46621 taatatcgaa ttctttgccg ccaccatcga atccaaagca aagctggtgt atgaccaggt
    46681 ttctgactgg ctggaaaata ccggtgactg gcagcctgaa agtgaagcaa ttgccgaaca
    46741 agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
    46801 ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
    46861 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
    46921 taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
    46981 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
    47041 gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc gtcgtgaact
    47101 ggacgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
    47161 aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg ccacctggac
    47221 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
    47281 aggcgaaact gcgacgcgcc cacaggatga aatcactgtc caaatggccg agcgtcgccg
    47341 tctcaaccgg atggcagaac gtgatgttgg tgactggtta tacgcacgct tcctgaaaga
    47401 caaagccggg accgacaccc gtttcgcagc ggaaattgtc gatatcagcc gtggcggcat
    47461 gcgtgttcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
    47521 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
    47581 ggtttacaaa gtgactgacg ttattgacgt cactattgcc gaagtccgca tggaaacccg
    47641 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct catggatggg
    47701 ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac tgactttcgt
    47761 ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa aaacaatgcg
    47821 tttagccgcc gttaatttca tgtaagtcat gttgttcctt ttatttaacc gcggactccg
    47881 cagttaaccg gaggatatgc atcatgaaaa ccgttaggga gtccacaacg ttgtacaact
    47941 ttctcggatc gcacaatcca tactggcggt tgacggaaag cagcgatgtt ttgcgctttt
    48001 ctaccaccga aaccacagaa cctgatcgta ctttgcagtt atctgccgaa caggctgctc
    48061 gcatcaggga aatgacggtc atcacctcca gcctgatgat gagtctgacc gtcgatgaga
    48121 gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa acgggaatgg gctggcaacg
    48181 cgtctgcatg gcatgacaca ccggcggttg ctcgtgattt atcacacggg ctttcctttg
    48241 ctgagcaggt agtttctgaa gcacattccg caatagtcat tctcgacagc cgggggaata
    48301 ttcaacgctt caatcggtta tgtgaagatt acacagggtt gaaagaacac gacgtcattg
    48361 ggcaaagcgt gtttaaactg tttatgagcc gtcgtgaagc tgcggcatcc aggcgcaata
    48421 accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga actgtggata ccaacatgta
    48481 aaggccagcg gctgtttctg tttcgcaata aatttgtcca cagcggcagt ggcaaaaacg
    48541 agattttttt aatctgttcc ggcaccgaca ttaccgaaga gcgccgcgct caggagcgac
    48601 tgcgtattct ggcaaatacc gacagtatca ccggactgcc gaatcgtaac gcaatgcagg
    48661 atttaatcga tcacgctatt aatcatgcag ataacaataa agttggggtt gtgtatcttg
    48721 atttggataa tttcaaaaag gtcaatgacg cctatgggca tttgtttggt gaccagttat
    48781 tacgcgacgt gtcattggct attttaagct gtctcgaaca tgaccaggtg ttggcgcgtc
    48841 caggtgggga tgagtttctg gtactggcat ccaacacctc acaaagcgcg ctggaagcaa
    48901 tggcatcacg aattttgacc cgcttacggc tcccctttcg cattggttta attgaagttt
    48961 ataccagctg ttcagtaggt attgcactct ctcccgaaca tggttcagac agcacggcta
    49021 ttattcgtca cgccgacaca gcaatgtaca cagcgaagga aggcggacga ggacaatttt
    49081 gcgtttttac tccagaaatg aatcagcggg tatttgaata tctctggctg gataccaact
    49141 tgcgtaaagc actggaaaac gatcagttgg ttattcacta tcaaccgaaa atcacctggc
    49201 gtggcgaagt gcgcagtctg gaagcactag tacgttggca gtcacctgaa cgtgggttga
    49261 ttccaccgtt ggacttcatt tcctacgccg aagagtcagg gctaattgtg cctttaggcc
    49321 gttgggtgat tctcgatgtc gtacgccagg tggcaaagtg gcgggataaa ggcataaacc
    49381 tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga tcaaaccatt ttcaccgccc
    49441 tgaaacaggt tctccaggaa ctcaattttg aatactgccc tatagatgtt gaactgacag
    49501 agagttgtct gattgagaat gatgaactgg cactgtctgt tattcaacaa tttagccaac
    49561 taggtgcgca agtgcatctg gatgattttg gtaccggcta ctcttcactt tcgcaactgg
    49621 cgcgctttcc gatcgatgcc atcaaacttg accaggtttt tgttcgagat attcacaaac
    49681 aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt ggcccaggca ttgaatcttc
    49741 aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc ttttttaacc aagaacggga
    49801 tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc cgccgtcgcc ttcgaacgct
    49861 ggtataaacg ctatctgaag cgcgcataag tagatgaata tatatactta gcgctatatc
    49921 ccgtcattat atatcgtatt cctggatgat aattagccgt tgctgttaaa cacttctctt
    49981 ttagcggcaa tttttgcatt attcattttc cacttttgct ataaggatgt ccttgatgtc
    50041 tgaatttgat gcccagcgcg ttgccgaacg tattgatatt gtgttagata tccttgtggc
    50101 aggcgattac cactctgcga tccataatct cgagatactt aaagcagaac tcttgcgcca
    50161 ggttgctgaa tctacgccag acatcccgaa ggcaccgtgg gagatttaac aatcgtgcct
    50221 tcaggttttc ctatttgagg atgcccggcg cgtcagtatt tttttattta gtattataac
    50281 gttataagaa ttacagcgat gaattcccga caacaaacta ttctacagat ggtcattgac
    50341 cagggtcagg ttagcgtaac cgatctggca aaagccactg gagtttctga agttaccatt
    50401 cgccaggatc tcaacaccct cgaaaaactg agttacctcc gccgtgcaca tggctttgca
    50461 gtttcgcttg atagtgatga cgtcgaaacc cgtatgatga gcaactatac gctgaagcgt
    50521 gaactcgccg agtttgccgc gtcactggtt caaccgggcg aaaccatctt tatcgaaaat
    50581 ggtagcagca atgccctgct cgcccggact ctgggcgaac agaagaaaaa tgtcactatc
    50641 atcacagtca gcagctacat tgcgcatttg ctgaaagacg cgccttgtga agtcattttg
    50701 ctcggtggcg tgtaccagaa aaaaagcgaa agtatggttg gccctttgac acgccagtgc
    50761 atccagcagg tacatttcag caaagcattt attggtattg atggctggca acctgaaact
    50821 ggattcaccg gtcgcgatat gatgcgtacc gatgtggtca atgccgtgct ggaaaaagag
    50881 tgcgaagcga tagtcctgac tgacagctcg aaatttggtg ctgtacattc atactccatc
    50941 ggtcccgttg agcgattcaa tcgcgtgatt accgattcga aaatacgcgc cagcgatctg
    51001 atgcatcttg agcacagtaa actcaccgtt cacgtcgttg acatttaacc atttcccttc
    51061 tctacggatg atttgcagtt tggcaaatca tccgctctaa gatgattcct ggttgataat
    51121 taagactatt tacctgttat taacactctc aagatataaa attattatca gcgatataac
    51181 aggaagtcat tatcacctgc gtgatataac cctgcgcgcg agcagatttc acggaataat
    51241 ttcaccaggc ttattcttag ctattatagt tatagagagc ttacttccgt gaatcataaa
    51301 ttcaggagag agtattatgt ttgtaacgag caaaaaaatg accgcggctg ttctggcaat
    51361 tactttggca atgtctctga gtgcctgttc taactggtct aaacgggacc gcaacaccgc
    51421 aatcggcgcg ggtgcagggg cattaggcgg tgcagtactg accgatggca gtacgttggg
    51481 tacattaggt ggtgcagctg tcggtggtgt tattggtcac caggtcggta aataatcgta
    51541 aataaatgtc gattatcgac tcgcaattat aatacgcttg attctgttca gtacgaaata
    51601 actgaataat acgcacagta aaatattcga aagccacgga tatatccgtg gctttttatg
    51661 ttaaccgcct gcgagtttta ccttcatccc tttcgcttca agtagtgatt ttaataaatc
    51721 acgcttatcg ccctggattt caataactcc atctttaact gctccgccgc agccgcattt
    51781 tttcttcagt tccgctgcaa gttttgtcag ttcggcatca tcgagatcga caccggtaat
    51841 caggcaaacg cccttacctt tacgtccact ggtctgacgc tgaatacgca ccacaccgtc
    51901 gcctttaggg cgtacagggg ccgctttggg ctcatcaata cgtccggttt ccgttgagta
    51961 gaccagacgg ctgttggaat cactcatcat gcactccgct gtaaagaggc gttgatcgct
    52021 ttcagcgtct gcgctggatc taccgattgc gttaccgggc gaccaatcac catataatca
    52081 acaccagccg acaacgcctg ttctggcgtc ataatgcggc gctggtcacc agcttcactc
    52141 ccctgcggac gaatgcccgg cgtaaccagt ttgaactcct gaccgaatac ctgtttaaag
    52201 cgcacagctt cctgagcaga acacaccaca ccatcaaggc tacatttttg cgtcagtgcc
    52261 gccagacgtt ctgcataatc tgcaggtgac agtgtcatgc caagatcgac caggtcgctg
    52321 gcttccatgc tggtcaacac tgtcacagca atcaaaagcg gtgcatcttt gccaaacgga
    52381 accagtgcct cacgcgctgc ggtcatcata cgcgccccac cagaggcatg aacattcacc
    52441 atccacacgc ctaagtcagc tgcagcagcg acagcgtgcg ctgcagtgtt aggaatatcg
    52501 tggaatttca ggtcaagaaa gatatcaaaa ccacgctgtt gaagttcgcg cacaaactgt
    52561 ggcccaaaca atgtaaacat ctctttgccg accttcagac gacaatcgcg tgggtcgatc
    52621 ttgtcgacaa aggacagcgc gtcatcacga ttatgataat caagggcaac aaccacagga
    52681 gaattcgtaa cagcgcggga agaagatgaa gcagttaacg tcatgaccag accttcttga
    52741 tgatgggcac cggaagtggc gcgaaacagg taaacggcga gcattctacc cgtggaaata
    52801 aaaaattaac agttgcgatt tttcccctgg caggcttttt ctgctccgtc gttgccggag
    52861 cacaatggaa catcaataac tagtatgttg taactaaagt cggatttttt taaaaattac
    52921 aggccatcaa gaccgcgaat cggtttaatg gttgaccagg cccgacaaga cggacaatgc
    52981 cagtagaggg tgtatgcggt aaaaccacat ttctggcagc gataacgagg cttactacgt
    53041 accttttcgc caaccatgtc acgcagcacc atcaggctct ctttggcacg cccttcttcc
    53101 gcttcattta agtggtaatc cattaactta tggaacacac gcatggtcgg atgacgctga
    53161 agctggcgcg taatatagat ctgtgcagcc tcactaccgt cgcgcgcttc gatgatatcc
    53221 gcaagcatca attcagcatc ggcaccggtg ttctcttcca ccgcgcgttg caggaattct
    53281 gcccattcgg cagttttacc caactgctga tagcaggttt gcaacatttc cagcgtttcg
    53341 ctgaccagtt ctctgtcctg ggatatcacg cgttgcaaac tttcgacggc tttggcgtat
    53401 tctcctttcg ccataaacac gcgtcccatc atgatggata cgcgggcgct gtttttatct
    53461 gccgccgccc ctttttttag caaggtcatg gcacgatcga gatcgtcgct ggccatatgc
    53521 tgcagggcta actcacagta gaaatgggca atttcgacgc gctgtttatc tttacccagc
    53581 ttcaccaggc gttcggcaac atcaattgct ttctgccact cactggtagc ctggtagatt
    53641 tgtagcaact gttgcagagc gccaatgcgg aagtcagttt catcggtcag ctgattgaac
    53701 atgtcttccg cgcggtcgta taacccggcg gccatgtaat cacgccctag ttgttgaatc
    53761 gccaacagac gctgttcata ggtcagcgag gcgctttcca ttagggtctg atggatgcga
    53821 atagcgcgat caacttcgcc acgcgaacgg aacaggtttc cgagcgtaag gtgggcttca
    53881 acggtgcctg tatcctcttt aagcatatcg agaaacaggt ctaccgcttt atcctgttga
    53941 ttactaagca ggaagttaac ccccgctacg taatcacgcg acaagcggtt ggcttcatct
    54001 tgcttatttt gttgcgcact tctgcggccc atataccagc cataggcagc ggctacaggc
    54061 aaaagcagaa acaacaactc cagcatagaa agttattcct tcgccgctga cgagtgcggc
    54121 actacagcca cgtcagtcgc gggtgaaagc tggttttcca gtcgctttat tttacgttca
    54181 gcgcgcgcca gggaaacacg aactcgcagc cagaacaggc cacaaatcaa ccaaccgata
    54241 gcaaaccccg cagcaaacaa taccgccagc aatgtggaaa tacggtactc cccttgcgct
    54301 aacagataat taaacgtcac ctgttgatcg ttctgcgcac ccaacgtgac cgaaatcacg
    54361 aagatcgcta acaccagtaa gaaaatgagt aaatatttca cattacttcc cgttatgtgg
    54421 ttagagcgaa taaattatgg tcaacttacc gcaatagccc tttaaagtac cattttcgcg
    54481 ccgtctgcga aatggcatcc acgcgcattg ttgatgattt aagggctaaa ttcggaaaat
    54541 caaccagcgt taactttctt gttctcgttg cgctatttcg cgattttctt ccgcaggtgg
    54601 tgttaatggc ccacaaattc gttgcgcaag ccacgttgcc accgccacca gcgcccacga
    54661 aatcaacgta gctactacca gatcgcgtgg ccaatgcatc ccgagcagca ggcggcttcc
    54721 catgactccc gttgcccaga ccagcaagat agcaatggtt aacgttcgcc gacgcggcca
    54781 cagcaaacca acggccagca gtgcccaact ggcagcaaac atcgtgtgac cggaaggaaa
    54841 ggcaaacccc gtctctttct gccagtgtga acgcaaatat tgtgggatat ttttctcttc
    54901 agccaactgt tctttcacta aatttccgcg ttctgctcgc tttaaagtgt agaactcatc
    54961 aaccggaata tgatgtgttt tttccagcca gataacaaaa ggtcgtggtt cctggacttt
    55021 gtctttgatc caggatttaa cgccttgtcc cacaaggatt gcggccgcca gaatggcaaa
    55081 taatacaaag gcagccttaa tgcgaaaacg cagacaccag agaaaccagc cgaataaaat
    55141 caaatgtgta atgacgcccc agggctgggt gacagtttca gtaacccaaa aagccgcttt
    55201 tagtagccaa ctttgttctc caggttgcca acgccagcca gaaatccata cggctactgg
    55261 catgacaagc aatagtgcag ctcccactgc ggtacgtctg gcaatcgaac gcatggcctc
    55321 tcctttttga taagtcccac aatcataact gaaaacgcca gttccaggaa aaattgacag
    55381 atttgtgcca ttccgtgaac gatcgacgcg tcgtgattag gtgaacccct tctcgttatg
    55441 gcaaaataag ccaatacaga accagcatta tctggagaat ttcatgcagc ttaaacgtgt
    55501 ggcagaagcc aaactgccaa ccccatgggg cgatttcctg atggtgggat ttgaagaact
    55561 ggcaaccgga cacgatcatg tcgcgctagt ctatggcgat atttccgggc ataccccggt
    55621 acttgcgcgc gtccattccg aatgtctgac cggtgacgcc ctgttcagct tgcgctgcga
    55681 ttgtggcttc cagctcgaag cggcattgac gcaaattgcc gaggaaggcc gtggtatttt
    55741 gctgtatcac cgtcaggaag gtcgtaacat tggtctgctg aataaaatcc gcgcttacgc
    55801 actgcaggat caaggttacg ataccgtaga ggctaaccac cagttaggct tcgccgctga
    55861 tgagcgcgac ttcactcttt gcgctgatat gttcaaactc cttggcgtca atgaagtccg
    55921 cttgttaacc aataacccga aaaaagtcga aattctgacc gaagcaggga ttaatattgt
    55981 tgaacgcgta ccattgattg taggtcgtaa ccccaataac gaacattatc tcgatactaa
    56041 agccgagaaa atgggccatt tgctgaacaa ataaccctct tgcattgtgt aattagtttg
    56101 cttgccggaa gcaaaataac cggcaagcaa atacttgtta cttcaacata ttacgaatga
    56161 cataatgcaa aatgccgtcg ttctggtagt aggtcaactc cgtcgcggtg tcgatacgac
    56221 aacggcaggg tacgacctcc tggctaccat ccgcgcgcgt aagcgtcacc ggaaccgtcg
    56281 caccgggttg tagattttgc agatcgccaa tatcaatctt ctcttccccg gttagtccta
    56341 acgttttacg cgttacgcct tgcggaaatt ccagcggcag gatgcccatg ccaattaaat
    56401 tcgaacggtg aattcgttca aacgattcgg caatcaccac acgaattcca agcagacgag
    56461 gaccttttgc cgcccagtca cggcttgagc ctgatccata ctctttcccg gcaatcaccg
    56521 ccagcggcgt ttgttcctgc ttatagcgca tcgcagcatc ataaatagag actacgtcgc
    56581 tgtcaggtaa atgccgcgtc atcccccctt caacgccagg caccatttca ttacggatgc
    56641 gaatattggc gaaggtgccg cgcatcatca cttcatggtt accacgccgc gaaccgtagg
    56701 agttaaagtc ttttcgctca acaccccgac cttgtagata tcgacccgct gggctgtcgg
    56761 gcttaatact gcccgccgga gagatatggt cagtggtgac tgaatccccc agcattgcga
    56821 ggatccgcgc accgtgaata tcttccactg gtgctggtgt tgcctgcatt tcatcaaaga
    56881 aaggcgataa gcgaatatag gttgagtcct cctgccaacc gtaggtatcg gatcgtgtga
    56941 cgttaatttc cttccactct gctgtgcctt caaaaacttc tgcgtactct ttgcggaaca
    57001 tttctgtgga gacttgttct accgcacggg caatttcttg tgccgatggc cagatatctt
    57061 tcagataaac cgggtcgcct ttacgatcat gaccaatagg ctcagcagcc aggttgatat
    57121 tcatatttcc cgccagcgca taggcaacca ccagcggcgg cgaggccagc cagttagttt
    57181 taaccagcgg atggatacgg ccttcaaagt tacggttgcc ggacagcacc gcaccgacgg
    57241 ttaaatcgcc ttttttgatt gccgtttcga taggatcggg cagcggccca gagttaccaa
    57301 tacaggtggt acaaccgtat cccacaaggt taaaccccag ttcgtcgaga tacggtgtca
    57361 gtttcgcttt tgccagataa tcagaaacga ctttcgaacc cggtgccagc gacgctttga
    57421 cccacggttg ccgcttgagg cccagagtta cggctttttt cgccagcaag cctgcggcca
    57481 tcagcacact tgggttagag gtgttggtgc acgaggttat cgcagcaatg accacagcgc
    57541 catcaggtaa ctgatactga tgtccgttca taacataatc gaccggctgg cgatttttat
    57601 gcgtggcatt cacttccagt tcgttactgg cggcaaatgc ttttggtaca tcgggcagtg
    57661 caacgcgatc ctgtgggcgt tttggccctg ccaggctcgc ttcaacgtca ttcatatcca
    57721 gttctaacgt actggtaaaa atcggttcat cgcccgggtt acgccacatg ccctgcgctt
    57781 tggcatattt ttcgaccaac tcaacctgat cttcgctgcg cccgcttaaa cgcatgtaat
    57841 cgagggttac agcatcgatt gggaagaagc cacaggtggc accatattct ggcgacatat
    57901 tggcaatggt ggcgcgatcc gccagcggta gtgaatccag accatcacca taaaattcga
    57961 cgaatttccc caccacgcca tgtttgcgca gcatttgggt aacagtgaga accaggtctg
    58021 tggcggtaat accttcacgt aatttaccgg taagtttaaa gcccactacg tccgggataa
    58081 gcatggaaac cgg
//
LOCUS       DAZFYC010000012        57886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFYC010000012 DAZFYC010000000
VERSION     DAZFYC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.012"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..454)
                     /gene="folK"
                     /locus_tag="ACHKW5_000924"
     CDS             complement(<1..454)
                     /gene="folK"
                     /locus_tag="ACHKW5_000924"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO7510361.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTR"
     gene            complement(451..1848)
                     /gene="pcnB"
                     /locus_tag="ACHKW5_000925"
     CDS             complement(451..1848)
                     /gene="pcnB"
                     /locus_tag="ACHKW5_000925"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO7510362.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1908..2804)
                     /gene="gluQRS"
                     /locus_tag="ACHKW5_000926"
     CDS             complement(1908..2804)
                     /gene="gluQRS"
                     /locus_tag="ACHKW5_000926"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO7510363.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2871..3326)
                     /gene="dksA"
                     /locus_tag="ACHKW5_000927"
     CDS             complement(2871..3326)
                     /gene="dksA"
                     /locus_tag="ACHKW5_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO7510364.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3504..4208)
                     /gene="sfsA"
                     /locus_tag="ACHKW5_000928"
     CDS             complement(3504..4208)
                     /gene="sfsA"
                     /locus_tag="ACHKW5_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO7510365.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4223..4753)
                     /gene="thpR"
                     /locus_tag="ACHKW5_000929"
     CDS             complement(4223..4753)
                     /gene="thpR"
                     /locus_tag="ACHKW5_000929"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO7510366.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4827..7256
                     /gene="hrpB"
                     /locus_tag="ACHKW5_000930"
     CDS             4827..7256
                     /gene="hrpB"
                     /locus_tag="ACHKW5_000930"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO7510367.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGFLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7452..9986
                     /gene="mrcB"
                     /locus_tag="ACHKW5_000931"
     CDS             7452..9986
                     /gene="mrcB"
                     /locus_tag="ACHKW5_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO7510368.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10206..12449
                     /gene="fhuA"
                     /locus_tag="ACHKW5_000932"
     CDS             10206..12449
                     /gene="fhuA"
                     /locus_tag="ACHKW5_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO7510369.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12500..13297
                     /gene="fhuC"
                     /locus_tag="ACHKW5_000933"
     CDS             12500..13297
                     /gene="fhuC"
                     /locus_tag="ACHKW5_000933"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO7510370.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIVQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13297..14187
                     /gene="fhuD"
                     /locus_tag="ACHKW5_000934"
     CDS             13297..14187
                     /gene="fhuD"
                     /locus_tag="ACHKW5_000934"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO7510371.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDRDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14184..16166
                     /gene="fhuB"
                     /locus_tag="ACHKW5_000935"
     CDS             14184..16166
                     /gene="fhuB"
                     /locus_tag="ACHKW5_000935"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO7510372.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16201..17481)
                     /gene="hemL"
                     /locus_tag="ACHKW5_000936"
     CDS             complement(16201..17481)
                     /gene="hemL"
                     /locus_tag="ACHKW5_000936"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO7510373.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17647..17694
                     /gene="yadX"
                     /locus_tag="ACHKW5_000937"
     CDS             17647..17694
                     /gene="yadX"
                     /locus_tag="ACHKW5_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO7510374.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17706..19127
                     /gene="clcA"
                     /locus_tag="ACHKW5_000938"
     CDS             17706..19127
                     /gene="clcA"
                     /locus_tag="ACHKW5_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO7510375.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19151..19216
                     /gene="yadW"
                     /locus_tag="ACHKW5_000939"
     CDS             19151..19216
                     /gene="yadW"
                     /locus_tag="ACHKW5_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO7510376.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19209..19553
                     /gene="erpA"
                     /locus_tag="ACHKW5_000940"
     CDS             19209..19553
                     /gene="erpA"
                     /locus_tag="ACHKW5_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO7510377.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19600..20223)
                     /gene="yadS"
                     /locus_tag="ACHKW5_000941"
     CDS             complement(19600..20223)
                     /gene="yadS"
                     /locus_tag="ACHKW5_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO7510378.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20261..21061)
                     /gene="btuF"
                     /locus_tag="ACHKW5_000942"
     CDS             complement(20261..21061)
                     /gene="btuF"
                     /locus_tag="ACHKW5_000942"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO7510379.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21054..21752)
                     /gene="mtnN"
                     /locus_tag="ACHKW5_000943"
     CDS             complement(21054..21752)
                     /gene="mtnN"
                     /locus_tag="ACHKW5_000943"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO7510380.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21836..23353
                     /gene="dgt"
                     /locus_tag="ACHKW5_000944"
     CDS             21836..23353
                     /gene="dgt"
                     /locus_tag="ACHKW5_000944"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO7510381.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23483..24907
                     /gene="degP"
                     /locus_tag="ACHKW5_000945"
     CDS             23483..24907
                     /gene="degP"
                     /locus_tag="ACHKW5_000945"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO7510382.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25062..26219
                     /gene="cdaR"
                     /locus_tag="ACHKW5_000946"
     CDS             25062..26219
                     /gene="cdaR"
                     /locus_tag="ACHKW5_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO7510383.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARLLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTELDLGNFDDRLLLYVALQLDEER"
     gene            complement(26308..26694)
                     /gene="yaeH"
                     /locus_tag="ACHKW5_000947"
     CDS             complement(26308..26694)
                     /gene="yaeH"
                     /locus_tag="ACHKW5_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO7510384.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27006..27830)
                     /gene="dapD"
                     /locus_tag="ACHKW5_000948"
     CDS             complement(27006..27830)
                     /gene="dapD"
                     /locus_tag="ACHKW5_000948"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO7510385.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27861..30533)
                     /gene="glnD"
                     /locus_tag="ACHKW5_000949"
     CDS             complement(27861..30533)
                     /gene="glnD"
                     /locus_tag="ACHKW5_000949"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO7510386.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30595..31389)
                     /gene="map"
                     /locus_tag="ACHKW5_000950"
     CDS             complement(30595..31389)
                     /gene="map"
                     /locus_tag="ACHKW5_000950"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO7510387.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31757..32482
                     /gene="rpsB"
                     /locus_tag="ACHKW5_000951"
     CDS             31757..32482
                     /gene="rpsB"
                     /locus_tag="ACHKW5_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO7510388.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32740..33591
                     /gene="tsf"
                     /locus_tag="ACHKW5_000952"
     CDS             32740..33591
                     /gene="tsf"
                     /locus_tag="ACHKW5_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO7510389.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33738..34463
                     /gene="pyrH"
                     /locus_tag="ACHKW5_000953"
     CDS             33738..34463
                     /gene="pyrH"
                     /locus_tag="ACHKW5_000953"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO7510390.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34755..35312
                     /gene="frr"
                     /locus_tag="ACHKW5_000954"
     CDS             34755..35312
                     /gene="frr"
                     /locus_tag="ACHKW5_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO7510391.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35404..36600
                     /gene="ispC"
                     /locus_tag="ACHKW5_000955"
     CDS             35404..36600
                     /gene="ispC"
                     /locus_tag="ACHKW5_000955"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO7510392.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36789..37547
                     /gene="ispU"
                     /locus_tag="ACHKW5_000956"
                     /gene_synonym="uppS"
     CDS             36789..37547
                     /gene="ispU"
                     /locus_tag="ACHKW5_000956"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO7510393.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37560..38417
                     /gene="cdsA"
                     /locus_tag="ACHKW5_000957"
     CDS             37560..38417
                     /gene="cdsA"
                     /locus_tag="ACHKW5_000957"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7510394.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38429..39781
                     /gene="rseP"
                     /locus_tag="ACHKW5_000958"
     CDS             38429..39781
                     /gene="rseP"
                     /locus_tag="ACHKW5_000958"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO7510395.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39811..42243
                     /gene="bamA"
                     /locus_tag="ACHKW5_000959"
     CDS             39811..42243
                     /gene="bamA"
                     /locus_tag="ACHKW5_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO7510396.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42365..42850
                     /gene="skp"
                     /locus_tag="ACHKW5_000960"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42365..42850
                     /gene="skp"
                     /locus_tag="ACHKW5_000960"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO7510397.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42854..43879
                     /gene="lpxD"
                     /locus_tag="ACHKW5_000961"
     CDS             42854..43879
                     /gene="lpxD"
                     /locus_tag="ACHKW5_000961"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO7510398.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43984..44439
                     /gene="fabZ"
                     /locus_tag="ACHKW5_000962"
     CDS             43984..44439
                     /gene="fabZ"
                     /locus_tag="ACHKW5_000962"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO7510399.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44443..45231
                     /gene="lpxA"
                     /locus_tag="ACHKW5_000963"
     CDS             44443..45231
                     /gene="lpxA"
                     /locus_tag="ACHKW5_000963"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO7510400.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45231..46379
                     /gene="lpxB"
                     /locus_tag="ACHKW5_000964"
     CDS             45231..46379
                     /gene="lpxB"
                     /locus_tag="ACHKW5_000964"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO7510401.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46376..46972
                     /gene="rnhB"
                     /locus_tag="ACHKW5_000965"
     CDS             46376..46972
                     /gene="rnhB"
                     /locus_tag="ACHKW5_000965"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO7510402.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47009..50491
                     /gene="dnaE"
                     /locus_tag="ACHKW5_000966"
     CDS             47009..50491
                     /gene="dnaE"
                     /locus_tag="ACHKW5_000966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO7510403.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50504..51463
                     /gene="accA"
                     /locus_tag="ACHKW5_000967"
     CDS             50504..51463
                     /gene="accA"
                     /locus_tag="ACHKW5_000967"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO7510404.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51562..53703
                     /gene="ldcC"
                     /locus_tag="ACHKW5_000968"
     CDS             51562..53703
                     /gene="ldcC"
                     /locus_tag="ACHKW5_000968"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO7510405.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     VQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53760..54149
                     /gene="yaeR"
                     /locus_tag="ACHKW5_000969"
     CDS             53760..54149
                     /gene="yaeR"
                     /locus_tag="ACHKW5_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7510406.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54214..55512
                     /gene="tilS"
                     /locus_tag="ACHKW5_000970"
     CDS             54214..55512
                     /gene="tilS"
                     /locus_tag="ACHKW5_000970"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO7510407.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55561..55821)
                     /gene="rof"
                     /locus_tag="ACHKW5_000971"
     CDS             complement(55561..55821)
                     /gene="rof"
                     /locus_tag="ACHKW5_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO7510408.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55808..56008)
                     /gene="yaeP"
                     /locus_tag="ACHKW5_000972"
     CDS             complement(55808..56008)
                     /gene="yaeP"
                     /locus_tag="ACHKW5_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO7510409.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56174..56719
                     /gene="yaeQ"
                     /locus_tag="ACHKW5_000973"
     CDS             56174..56719
                     /gene="yaeQ"
                     /locus_tag="ACHKW5_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO7510410.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSVFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56716..57138
                     /gene="arfB"
                     /locus_tag="ACHKW5_000974"
     CDS             56716..57138
                     /gene="arfB"
                     /locus_tag="ACHKW5_000974"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO7510411.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57152..57862
                     /gene="nlpE"
                     /locus_tag="ACHKW5_000975"
                     /gene_synonym="cutF"
     CDS             57152..57862
                     /gene="nlpE"
                     /locus_tag="ACHKW5_000975"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO7510412.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13576 a        14351 c        16136 g        13823 t
ORIGIN      
        1 ctcttgtatg taagacttca cgcaacgtct ccccatcagg aaacgccagc tccggtgcaa
       61 tctcaaacag cggccacagc ataaatccac gattcttcat atcgtagtgc ggaacggtca
      121 ggcgttcggt atttatcact tcattaccaa acagcatgat atcgagatcc agcgtgcgtg
      181 gtccccagcg ttcagcttta cgaacacgac cctgttgcag ttctatgcgc tgtgtatggt
      241 tgagtaactc ttccggggcg agcgaagttt caagcgccac ggcggcgttg aggtaatcgg
      301 gttgatcttg cggccccagc ggtggggtgc ggtaaaacga agaaacggca agaatgtggc
      361 tttcagggat atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca
      421 gattgctgcc tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg
      481 tttgcgtgga cgacgagtac gacgacgcgg tgacggttct tcatccaact cgttaagcat
      541 ccctttttga tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgctg
      601 cagttcagcg ttacgttcaa cttcagctcg caaggccaac aggtcataag ccgcacggaa
      661 cttaggatgc tccagcagtt tccatgcacg tttaccctga cgtcgggaca tacgcaactg
      721 caactgccag atatcgcggg ttaacgtcgt cagacgtttc gggattgcca gtgaacggca
      781 ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct
      841 ttcctgggcg atcttctgtg ccgtctccag cagtgggtac cagaacatgg cggcaaacag
      901 gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagcacctg
      961 ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg
     1021 gaacagcggc tggaacagat gatattcaca cagcagctta taggtttcgt aaccgtagcc
     1081 cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc ggtgggatat cgttcagcag
     1141 ggtagcgagg cgagggatcg gttctgcggt ttccgggctg atgcgcatac ccaatttggc
     1201 ggcaaaacgt accgcgcgca gcatacgtac cggatcttca cggtagcgcg tttccgggtt
     1261 accaatcaga cggataacgc cgtccttcag atccttcata ccgccaacgt aatcacggac
     1321 ggtaaaatcc gctacgctgt aatacaggct gttgatagtg aaatcgcggc gctgggcgtc
     1381 ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga
     1441 ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat
     1501 ctccgggcca aacatcacat gagccagacg gaaacggcga cccaccaggc ggcagttacg
     1561 gaacagtttg cgcacctgct caggcgtggc gttagtggtt acgtcaaagt ctttcggctt
     1621 tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt
     1681 gttgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg
     1741 ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc
     1801 ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg
     1861 tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca
     1921 tttgagaatg ttgaatttac aattgccgac tcgggcacgg cggtaagccg ccagtttttg
     1981 acggctgact gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc tgcctgctgc
     2041 cccagaaatt gaagtgccgc gattagtacc gggcgtggat cgcctttcgg caacgcaggc
     2101 gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg cagatgaatg
     2161 taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccttac tgttggttca
     2221 atcagatcag ccccacgcac tatttctgta acgccctgga aatgatcatc aaccacaaca
     2281 gccaggttgt aggcgaacaa cccatcacgg cgatgaatga taaaatcttc ccgtgccagt
     2341 ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc
     2401 tggcggatac gcactgcggc gttgtctggt ccatgatgca acacccggca atgaccgtcg
     2461 taaataccgc caatgctttg aatacgcgca cgcgtacagg tgcagtaata acttagtcct
     2521 tgttcatgta accaggcgag tgcttcacga taggcgtcgt gacgttgcga ttgccagaga
     2581 acatcgccgt cccagtgcag accgtaatgt tccagctggc gcaggatagt ttctgcggca
     2641 ccgggaactt cacgaggcgg gtcgatatct tctatgcgta ccagccagcg accttgccgg
     2701 gcgcgagcct gcaaatagct gccgagcgcg gcgatcagag agccaaaatg aagctcgccg
     2761 gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa
     2821 taaggcggga gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc
     2881 atctgttttt cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg
     2941 cgcgcttcca gacggcgaat accaatttca acaccgcagg attcgcagta gccgaaatct
     3001 tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg
     3061 cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg
     3121 aagttggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga
     3181 ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct
     3241 tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat
     3301 gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca
     3361 tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc
     3421 taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt
     3481 aaattaacca gttacttact ttactacaat gtaaccggca gtgatttttt aagagccatg
     3541 ccttcagcag aaatttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct
     3601 gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct
     3661 gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac gctcatcaac
     3721 tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc
     3781 tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac
     3841 ataaagtcaa tacggctgcg ttctgcgccg tattttactt cgcttttcag cgagctatag
     3901 cctgacagtt ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa
     3961 agcgtattga cgcaaataaa tgcgccgctc tggctttgag ttagttccca ggtgtgtggg
     4021 tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa
     4081 ccggtcatcg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc
     4141 acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagaa
     4201 aattccattg cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc
     4261 gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gcataagacc
     4321 agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat
     4381 gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca
     4441 tattcgccag ctggattaag ccgcgtggag gctgacgcat ccctaaccac accacacgcg
     4501 aacgcagcca ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc
     4561 cggctaaaag agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa aatgccagag
     4621 tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc
     4681 gccaatggat aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagacgtt
     4741 gcggttcaga catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga
     4801 agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttatctg
     4861 aattacttac cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg accggggccg
     4921 ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta
     4981 tcctgctgga gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacgactg gcggagctgc
     5041 ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc
     5101 cgaatacccg cctggaagtg gttaccgaag gcgtgctgac gcgcatgatc cagcgtgacc
     5161 cggaactgag cggtgttgga ttggtgatcc tcgatgaatt tcatgagcgc agcttgcagg
     5221 cggatttggc gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac
     5281 tgctgattat gtcggctacg ctggataacg accgcttgca gcaaatgctg ccagaagcgc
     5341 ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg ccattattta ccgctgcccg
     5401 cgcatcagcg ttttgacgaa gccgttgcgg tagccaccgc cgaaatgctg cgtcaggaaa
     5461 gcggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggagcaac
     5521 tggcttcgcg catcggcagt gatgtgttgc tctgcccgct gtatggcgcg ttgtcgctga
     5581 acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga
     5641 ccaatattgc tgaaaccagt ttaaccattg aaggtattcg tctggtggtg gattgtgccc
     5701 aggagcgtgt ggcgcgtttt gatccgcgca cggggcttac gcgactgatt actcaacgcg
     5761 ttagccaggc atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatca
     5821 gcctgcattt aatcgccaaa gaacaagcag aacgcgccgc ggcgcaaagt gaaccggaga
     5881 tcttacaaag cgatctttcc ggttttctga tggaattact gcaatgggga tgcagcgatc
     5941 cggcgcagat gagctggctg gatcaaccgc caacggtgaa tctactggcc gcgaaacgcc
     6001 tgttacaaat gctgggggcg ctggagggtg aacggcttag tgcgcagggg caaaaaatgg
     6061 cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg
     6121 aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata
     6181 gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac
     6241 tgttaaaacg cttaaacgta cgtggcggtg aggcagacag ttcgcttatc gcgccgctac
     6301 ttgccggggc gtttgccgat cgcattgctc gtcgccgtgg gcaagatgga cgctatcaac
     6361 tggcaaacgg catgggagcg atgctcgatg ccaacgacgc gctaagccgc cacgaatggt
     6421 tgatcgcacc gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgt
     6481 taccggtcga tattgatgaa ttagtacaac gctgtccgca gctggtgcag caatctgaca
     6541 ccgtggaatg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg caaatcggtc
     6601 agttgacggt gaaagtgcag ccactggcga aaccgtcaga agacgagttg catcaggcga
     6661 tgcttaacgg cattcgtgaa aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac
     6721 agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg
     6781 ttgatgatga aagtttattg gcgacgctgg aaacgtggct gctgccacat atgactggcg
     6841 tacattcact acgcggcctg aaatcactcg acatttatca ggcactacgc ggattacttg
     6901 attggggaat gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg
     6961 gaagccggat cgccattcgt tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc
     7021 aagagatgtt tggcgaggcc accaatccga cgatcgccca ggggcgcgtg ccgctggtgc
     7081 tggagttgct ttcacctgcc caaaggccat tacaaatcac acgagatttg agcgccttct
     7141 ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct
     7201 ggccggacga cccggcaaat actgcaccga cgcgacggac gaaaaagtat tcgtaaggtt
     7261 ggttttctcc ctctccctgt gggagagggc cggggtgagg gcatcagcgc gcacgttcac
     7321 cctcacccta accctctccc tcaagggaga ggggaccgat cgagcacaaa ttttgagaga
     7381 tatcttcttc tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc
     7441 ggagaaaaag catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc
     7501 gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt
     7561 atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc
     7621 gtaagcctcg tggcaaacgc ggctggctat ggctactgct aaaactggct atcgtttttg
     7681 ccgtgctgat cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg
     7741 gcaaggtctg gcaactgcct gcggcagttt atggccgaat ggtcaatctt gagccagaca
     7801 tgaccatcag caagaacgag atggtgaagc tgctggaggc gacccagtat cgtcaggtgt
     7861 cgaaaatgac ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgattcgcc
     7921 gtccgtttga tttcccggac agtaaagaag gacaggtgcg cgcgcgtctg acctttgatg
     7981 gcgatcatct ggcgacgatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc
     8041 ttgatccgcg tctgatcacc atgatctctt cgccaaacgg tgagcagcgt ctgtttgtgc
     8101 cgcgcagtgg tttcccggat ttgctggtgg atactttgct ggcgacagaa gaccgtcatt
     8161 tttacgagca tgatggaatc agtctctact caatcggacg tgcggtgctg gcaaacctga
     8221 ccgccggacg cacggtacag ggtgcgagta cgctgacgca acagctggtg aaaaacctgt
     8281 tcctctccag cgagcgttct tactggcgta aagcgaacga agcttacatg gcgctgatca
     8341 tggacgcgcg ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg
     8401 gtcagagcgg cgacaacgaa atccgcggct tcccgctggc aagcttgtat tactttggtc
     8461 gcccggtaga agagctaagc ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg
     8521 cgtccatcta caacccgtgg cgtaacccaa aactggcgct ggagcgacgt aatctggtgc
     8581 tgcgtctgct gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgcac
     8641 gtccgctggg tgttcagcca cgcggtgggg tgatctctcc acagccagcc tttatgcaac
     8701 tggtgcgtca ggagctgcag gcgaaattag gcgataaggt aaaagatctc tccggcgtga
     8761 agatcttcac tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag
     8821 gcattccggc actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg
     8881 tcgaccgctt tagtggtgaa gttcgtgcga tggtcggtgg ttctgagccg cagtttgcgg
     8941 gctacaaccg tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt
     9001 atctgactgc cttaagccag ccgaaaatct atcgtctgaa tacgtggatt gcggatgcgc
     9061 caatcgcgct gcgtcagccg aatggccagg tctggtcacc gcagaatgat gaccgtcgtt
     9121 atagcgaaag cggcagagtg atgctggtgg atgcgttgac ccgttcgatg aacgtgccga
     9181 cggtaaatct ggggatggcg ctggggctgc ctgcggttac ggagacctgg atcaaactgg
     9241 gcgtaccgaa agatcagttg catccggttc cggcgatgct gctgggggcg ttgaacttaa
     9301 cgccaatcga agtggcgcag gcgttccaga ccatcgccag cggcggtaac cgtgcaccgc
     9361 tttctgcact gcgttcggta attgcggaag atggcaaggt gctgtatcag agcttcccgc
     9421 aggcggaacg cgctgttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg
     9481 tggtacaacg cggtacgggt cgtcagcttg gggcgaaata cccgaacctg catctggcag
     9541 ggaaaacagg gactaccaac aataacgtag atacctggtt tgcaggcatt gacggcagca
     9601 cggtgaccat cacctgggta ggccgtgata acaaccagcc gaccaaactg tatggagcca
     9661 gcggggcaat gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc
     9721 ttgttccgcc agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca
     9781 gcggtggcat gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga
     9841 gcgagatgca gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac
     9901 agccgcaaca gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga
     9961 tcaaggatat gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc
    10021 atccggtaaa ataacatccc atctaagata ttaacccttt cttttcatct ggttgtttat
    10081 taacccttca ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat
    10141 tatcttatct ttataataat cattctcgtt tacgttatca ttcactttac atcagagata
    10201 taccaatggc gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg
    10261 tagtagccac agcggtaagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag
    10321 aagacactat caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg
    10381 caactattgc ggcgcgacag tctgctaccg gcactaaaac cgatacgccg attcaaaaag
    10441 tgccacagtc tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg
    10501 taaaagaagc gcttagctac acgccgggtg tctctgttgg tacgcgtggc gcatccaaca
    10561 cctatgacca cctgatcatt cgcggctttg cggcagaagg ccaaagccag aataactatc
    10621 tgaatggcct gaagttgcag ggcaacttct ataacgatgc ggtcattgac ccgtatatgc
    10681 tggaacgcgc tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg
    10741 gcggcctgtt gaatatggtc agcaagcgtc cgaccaccga accgctgaaa gaagttcagt
    10801 ttaaagccgg tactgacagc ctgttccaga ctggttttga ctttagcgat tcgttggatg
    10861 atgacggtgt ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga
    10921 aagggtcaga agagcagcgt tatgctattg caccggcgtt cacctggcgt ccggatgata
    10981 aaaccaattt caccttcctt tcttacttcc agaacgagcc ggaaaccggt tattacggct
    11041 ggttgccgaa agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact
    11101 ttaatgaagg ggcgaagaac aacacctatt ctcgtaatga gaagatggtc ggctacagct
    11161 tcgatcacga atttaacgac acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca
    11221 aaacctcgca aaacagcgtt tatggttacg gcgtctgctc cgatccggcg aatgcttaca
    11281 gcaaacagtg tgcggcatta gcgccagcgg ataaaggcca ttatctggca cgtaaatacg
    11341 tcgttgatga tgagaagctg caaaacttct ccgttgatac ccagttgcag agcaagtttg
    11401 ccactggcga tatcgaccac accctgctga ccggtgtcga ctttatgcgt atgcgtaatg
    11461 acatcaacgc ctggtttggt tacgacgact ctgtgccact gctcaatctg tacaatccgg
    11521 tgaataccga tttcgacttc aatgccaaag atccggcaaa ctccggccct taccgcattc
    11581 tgaataagca gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc
    11641 tggtcaccct gggcggtcgt tatgactggg cagatcaaga atctcttaac cgcgttgctg
    11701 ggacgaccga taaacgtgat gacaaacagt ttacctggcg tggtggtgtt aactacctgt
    11761 ttgataatgg tgtaacacct tacttcagct atagcgaatc gtttgaacct tcttcgcaag
    11821 ttgggaagga tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga
    11881 aatatgtacc ggaagatcgt ccgattgtag ttactggtgc cgtgtataat ctcactaaaa
    11941 ccaacaacct gatggcggac cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc
    12001 gcgcacgtgg cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg
    12061 gttcttatac ttacaccgat gcggaataca ccaccgatac tacctataaa ggcaatacgc
    12121 ctgcacaggt gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc
    12181 cgctttcagg tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc
    12241 cggctaactc ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc
    12301 tggcgcgagt cggcatggcg ggctccaacg tggcgctgca tgttaacaac ctgttcgatc
    12361 gtgaatacgt cgccagctgc tttaacactt atggctgctt ctggggcgca gaacgtcagg
    12421 tcgttgcaac cgcaaccttc cgtttctaat ttctcttttg gggcacggat ttccgtgccc
    12481 atttcacaag ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact
    12541 gcgtaatatc tcctttcgtg tgcccgggcg cacgcttttg catccgctgt cgttaacctt
    12601 tcctgccggg aaagtgaccg gtctgattgg tcacaacggt tctggtaaat ccactctgct
    12661 caaaatgctt ggccgtcatc agcctccgtc agaaggggag attcttcttg atgcccagcc
    12721 gctggaaagc tggagcagca aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct
    12781 tcctccggca gaagggatga ccgtgcgtga actggtggcg attggtcgtt acccgtggca
    12841 tggcgcgctg gggcgctttg gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct
    12901 ggttggctta aaaccgctgg cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca
    12961 gcgggcgtgg atcgccatgc tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc
    13021 gacctcggcg ctggatatcg cccaccaggt tgatgtgctg tcgctggtgc accgtttaag
    13081 tcaggagcgt ggcctgacgg tcattgccgt gttgcacgat atcaatatgg cggcacgcta
    13141 ctgtgattat ctggtcgccc tgcgcggcgg tgaaatgatt gttcagggaa cgcctgcgga
    13201 aattatgcgc ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta ttttgccgca
    13261 tccggcgggt gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg
    13321 cgccgtcgac tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc
    13381 cacgcggcgg ctattgatcc caatcgtatt gtggcgctgg agtggttgcc ggtggaatta
    13441 ctgctggcgc tcggcatcgt gccttacggc gtggcggata ccatcaacta tcgcctgtgg
    13501 gtcagcgaac caccattgcc ggactcagtg atcgacgtcg gtttgcgcac agaacctaac
    13561 cttgaactgc tgaccgaaat gaaaccatcg tttatggtct ggtcggcagg atatggccct
    13621 tcaccagaaa tgctggctcg tattgcgccg gggcgtggat ttaacttcag cgacggcaaa
    13681 cagccgctgg cgatggcgcg taagtcactg acggaaatgg cagatttact taacctgcaa
    13741 agcgcagcag aaacgcattt agcgcactat gaagacttta tccgcagcat gaaaccccgc
    13801 tttgtgaagc gtggtgaacg cccgttattg ctgacgacgc ttatcgatcc gcgccatatg
    13861 ctggttttcg gtccaaacag cttgtttcag gaaattcttg atgagtacgg catcccaaat
    13921 gcctggcaag gggaaaccaa cttctggggc agtaccgccg tcagcatcga tcgtctggcg
    13981 gcgtataaag atgttgatgt gctctgtttt gatcgcgaca acagcaaaga catggacgcg
    14041 ctaatggcaa ccccgctgtg gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc
    14101 gtacccgcag tctggtttta tggtgcgacg ctctcggcaa tgcactttgt gcgcgttctg
    14161 gataacgcca tcggaggtaa agcgtgagta aacgaattgc gctttttccg gcgttattgc
    14221 tggcgctgct ggtgattgtc gctacggcgc tgacctggat gaacttttcg caggcgctgc
    14281 cgcgtagcca gtgggcgcag gccgcctggt cgccggatat taacgtcatc gagcagatga
    14341 tttttcacta cagcttgtta ccacgtctgg cgatttctct actggtgggc gcgggcctgg
    14401 ggctggtggg cgtgctgttt cagcaagtgc tgcgtaaccc gctggcggaa ccgacgacgt
    14461 tgggcgttgc tacaggcgcg caactgggga ttaccgtcac cacgctctgg gcgatcccag
    14521 gtgctatggc gagccagttt gctgcgctgg caggggcttg cgttgttggc ttaattgtct
    14581 ttggcgtcgc gtgggggaaa cggctgtcgc cggtaacgct gattctcgcg gggctggtag
    14641 tgagccttta ttgtggggca atcaatcagt tactggttat cttccatcat gaccagttgc
    14701 aaagcatgtt cctgtggagc accggaacgc tgacgcaaac cgactggggc ggcgttgagc
    14761 gtttatggcc gcagttgctg ggcggcgtga tgctgacgtt actgctactt cgtccgttaa
    14821 ccctgatggg gcttgatgat ggcgtggcgc gcaatctcgg gctggccttg tcgctcgcgc
    14881 gtctggcggc gctgtcgctg gcgatagtca tcagtgcgct gctggtgaac gcagtgggga
    14941 ttatcggctt tatcgggttg ttcgcgccac tgctggcaaa aatgctgggg gcgcggcgtt
    15001 tgttgccacg gttgatgctg gcgtcgctga ttggtgcgtt aattctgtgg ctttctgatc
    15061 aaatcatcct ctggctgact cgcgtgtgga tggaagtttc caccggttcg gtcactgcgt
    15121 tgatcggtgc gccgctgcta ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg
    15181 atatgaaggt taacgatcgt gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg
    15241 cgggcggcgt gctgctgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc
    15301 acggctggac gtgggcgagc ggggcgctgc tcgatgattt aatgccctgg cgctggccgc
    15361 gaattatggc ggcgctgttt gcgggcgtca tgctggcggt agcgggctgt attattcagc
    15421 ggctgaccgg caacccgatg gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt
    15481 ttggtgtggt gttaatgctg tttctggtac cgggtaatgc ctttggctgg ctgttacccg
    15541 caggcagtct cggggcggcg gtgacgctgt tgatcattat gatcgccgcc ggacgcggtg
    15601 gattttcccc acaccgcatg ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc
    15661 ttttgatgat gttgcaggca agtggtgacc cgcgaatggc gcaagtgctg acctggattt
    15721 ccggttcgac ctacaacgcg accgatgcgc aggtctggcg caccggaatt gtgatggtga
    15781 ttttgctggc gattaccccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg
    15841 ataccgcccg agccgtagga atggcgctga cgccgacgcg aattgcgctg ctgctgttag
    15901 cggcttgcct gacggcgacc gcgacgatga ctattggacc gttgagtttt gttggtttaa
    15961 tggcaccgca tattgcgcgg atgatgggct ttcgacggac gatgccacac atcgtaattt
    16021 cggcgctggt gggggggtta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt
    16081 ttccattcca gatcccggcg gggctgctgt caacctttat cggcgcgcca tattttatct
    16141 atttgttgag aaagcagagc cgttaattga atgcctcgcc cggtgggcga ggcaaaatca
    16201 tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg ttattgatat cttccatgct
    16261 gtgcgccacg gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc
    16321 cagcatcata tggaagaaac gcttaaagcg ttccacgtca caggccatca catcctgata
    16381 gcacgtcacg gactcggcgt cggtaaagaa aataccgaac atgccgccaa cgtggttaac
    16441 gaccagcgga attccggctt cttctgccgc ttccagcaga ccttctgcca gacgtgatgt
    16501 cagctcatcc aacgtttcgt gaacgcccgg ttgcgcgact tcattcagac aggcgaaacc
    16561 cgctgccatc gcaattgggt taccggaaag cgtacccgcc tgatagaccg gacccgtcgg
    16621 ggccagcgca tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc
    16681 gatgattttg cccaggcagg tgagatccgg ttccacaccg taataatcct gtgcgccagc
    16741 tagcgctacg cggaagccag tcattacttc atcgatgatc agcaatgcgc caaattcgtc
    16801 gcacagcgcg cgcagacctg gcaggaactc tggcagcggt ggaacgcagt tcatattgcc
    16861 tgccaccggc tcgacgataa tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg
    16921 tacagaagcc agatcgttat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac
    16981 acccggcgag tttggctggc ctaacgtgag tgcgccagaa ccggctttca ccagcaggca
    17041 gtcagcgtga ccgtggtaac aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc
    17101 acgggccagg cggatggcgc tcatggtcgc ttcagtgccg gagttcacca tgcgcaccat
    17161 atccatggtc gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggttgg
    17221 tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg gcattgcgga tcgccggatg
    17281 gttatggccc agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc
    17341 aacatcgtac agataggcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc
    17401 agtaaaggcg cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta
    17461 aagattttca gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta
    17521 ttctatgtta ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc
    17581 aatcctgatt tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg
    17641 attttcatgg aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc
    17701 attggatgaa aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgta
    17761 gacaactgat tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg
    17821 cggcagtcgt cggcacgctt gtcgggctgg cagcggttgc ttttgacaaa ggtgtcgcct
    17881 ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgctactgt
    17941 taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca
    18001 aatacgcgcc cgaagcaggg ggatcgggga tcccggaaat tgaaggggcg ctggaagatc
    18061 aacgtcctgt tcgctggtgg cgtgtattgc cggtgaaatt ctttggcggg ctggggacac
    18121 tcggcggagg catggtgcta gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg
    18181 gtcgcatggt gcttgatatt ttccgcctga aaggtgatga agctcgccat acgctgctgg
    18241 caaccggtgc tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg ggtattttgt
    18301 ttattatcga agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat
    18361 ttattggcgt cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga
    18421 ttgacgtcgg taaactttct gatgcgccgc ttaatacgct gtggctttat ctgatcctcg
    18481 gtattatttt tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt
    18541 tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt actgatgggt ggtgcgattg
    18601 gcggtctgtg tggattgctg gggtttgtgg caccagcaac gtcgggcggc ggttttaacc
    18661 tgattcctat cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg
    18721 ctcgggtcat taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc
    18781 cgatgctggc gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt
    18841 ttccgcaata tcacctggag gcggggacgt ttgctattgc cggaatgggg gcattactgg
    18901 cggcatctat tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact
    18961 accagctcat tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta
    19021 ccggcgggaa accgctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg
    19081 agcaactggc gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg aaataccagg
    19141 gtattagata atggcgatta ttattgggtt agaatttgcc caattgccga tgtcgtttgg
    19201 agcaaaatat gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca
    19261 aagttaaaag cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca
    19321 ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag
    19381 gcgatatgac catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat
    19441 atctggtcgg cggttccgtt gattataccg aaggtctgga aggttctcgt ttcatcgtga
    19501 ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc taatctgttg
    19561 ttcatgtgcc ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag
    19621 cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac
    19681 gaccatgccc atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc
    19741 gtggacaata ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg
    19801 gatctcgcgg gccagtacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat
    19861 gcaaaccgcg attaacggac cggcttccgc attaaaggct ttattcacgc caatgccgac
    19921 aaacaccgcc agaccaacgg cgtccagcac cggcaacatc cattttggta agcgtcttgg
    19981 ctggcgcacc agcacgatgg tcagcatgct ggtgaccatt gcaacgacca gatcggtggg
    20041 atctttcacc caaaataccg ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc
    20101 tactgcggtt accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc
    20161 taacaaaacg ccggagatgg caaataccgc tgtgccgact atatccagcc aatagacgag
    20221 cattgttcaa tccccactga gcacctgaaa aggtcaggcg ctaatctacc tgtgaaagcg
    20281 cattacagag ctgttgtgca gcgaggataa tacgtgggct tgcacgttca aaccagtcac
    20341 tcgtgagagg aataacggga attttgagct gttcacccca gtattgtttg attttaggaa
    20401 tttggtccgg tccgcctgta atgacaatcg cctgtggcga gcgtgctaac acctgttcgc
    20461 ggctaacttg cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt
    20521 cgagtacctg gttctgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt
    20581 gcagaaaaac acgtttttta ggtttatcag catattgcgc tttcaattgc gcgtactgat
    20641 ccagcaggga ttgcgcggct tgttcggcct tgtctggttg cggactccag ggggccagtt
    20701 gacgtaacgc attggcaatt tgttcaatgc ttgtcgcatc gacccacatc acttttattc
    20761 ccagcgaagc cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca
    20821 gatcgggctt cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct
    20881 caatcttttg cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc
    20941 cggcagcaaa ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt
    21001 tgagccacag tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt gacttagcca
    21061 tgtgcaagtt tctgcaccag tgactcaacc atcaggctgg actgtttagc ggcaacagcc
    21121 aggaactcat cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg
    21181 acaacaaacg ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct
    21241 acagcaatgg cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg
    21301 aaagcgtcgc cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag
    21361 gcctcagcgg cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac
    21421 tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt gataacgtgc ttcgtccgag
    21481 acaacgatat cgcccacttt caacgttggt gccaggccac cggcagaacc ggtgttaata
    21541 atcacatctg gcttgcagtg ttccaacagc agagtggcac ccagcgccgc agcgacttta
    21601 ccgatgcccg atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg
    21661 caaccgccga gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct
    21721 tcttccattg caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga
    21781 aggcatagtt taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc
    21841 acagattgat ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt
    21901 taaaaccgaa catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc
    21961 tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt ccactggagc gcaatgccgc
    22021 cgtgcgcacg cgtcttaccc actcgatgga agtccagcag gtggggcgct acatcgccaa
    22081 agaaatttta agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact
    22141 gaccggacct tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc
    22201 gccgtttggt cattttggcg aagcggcgat aaatgactgg tttcgccagc gtttataccc
    22261 ggaagatgcc gaaagccagc ctctgactga cgatcgctgc agcgtggcgg cactacgttt
    22321 acgggacggg gaagaaccgc ttaacgagtt gcggcgcaag attcgtcagg acttatgtca
    22381 ttttgagggg aatgcacaag gcattcgtct ggtgcataca ttgatgagga tgaatctcac
    22441 ctgggcacag gttggcggta ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac
    22501 gcctgagaca catcactatt taatgaaaaa gccgggttat tatctttctg aagaagccta
    22561 tattgcccgg ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg
    22621 gattatggaa gctgccgacg acatctccta ttgtgtggca gaccttgaag atgcggtaga
    22681 gaaaagaata tttaccgttg agcagcttta tcatcatttg cacgaggcgt ggggtcagca
    22741 tgagaaaggt tcgctctttt cgctagtggt tgaaaatgcc tgggaaaaat cacgctcaaa
    22801 tagtttaagc cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa
    22861 taaactggta ccatacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg
    22921 aacgtttaat catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata
    22981 taaaaatgtc gctgtaaaac atgtgtttag ccatccggat gtcgagcagc ttgaattgca
    23041 gggctatcgg gtcattagcg gattattaga gatttatcgt cctttattaa gcctgtcgtt
    23101 atcagacttt actgaactgg tagaaaaaga acgggtgaaa cgtttcccta ttgaatcgcg
    23161 cttattccac aaactctcga cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt
    23221 accgtcagat tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgctgca
    23281 ggattatatc agcggtatga ccgacctcta tgcgtgggat gaataccgac gtctgatggc
    23341 cgtagaacaa taaccaggct tttgtaaaga cgaacaataa atttttacct tttgcagaaa
    23401 ctttagttcg gaacttcgcg ctataaaacg aatctgaaga acacagcaat tttgcgttat
    23461 ctgttaatcg agactgaaat acatgaaaaa aaccacatta gcactgagtg cactggctct
    23521 gagtttaggt ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac
    23581 gacagcccag cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt
    23641 cagcattaac gtagaaggta gcacaaccgt taatacgccg cgtatgccgc gtaatttcca
    23701 gcagttcttc ggtgatgatt ctccgttctg ccaggaaggt tcaccgttcc agagctctcc
    23761 gttctgccag gggggccagg gcggtaatgg cggcggccag caacagaaat tcatggcgct
    23821 gggttccggc gtcatcattg atgccgataa aggctatgtc gtcaccaaca accacgttgt
    23881 tgataacgcg acggtgatta aagtccagct gagcgatggt cgtaagttcg acgcgaagat
    23941 ggttggcaaa gatccgcgct ctgatatcgc gctgattcag atccagaacc cgaaaaacct
    24001 gaccgcaatt aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat
    24061 tggtaacccg tttggcctgg gagagacggt aacttccggg attgtctctg cgctggggcg
    24121 tagcggcctg aatgccgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg
    24181 tggtaactcc ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc
    24241 gatcctcgca ccggacggcg gcaacatcgg tatcggtttt gctatcccga gcaacatggt
    24301 gaaaaacctg acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat
    24361 tatggggact gagctgaatt ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg
    24421 tgctttcgta agccaggttc tgcctaattc ctccgctgca aaagcgggca ttaaagcggg
    24481 tgatgtgatc acctcactga acggtaagcc aatcagcagc tttgccgcac tgcgtgctca
    24541 ggtgggtact atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg acggtaagca
    24601 ggtcaacgtg aatctggaac tgcagcagag cagccagaat caggttgatt ccagctccat
    24661 cttcaacggc attgaaggtg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt
    24721 gaacaacgtg aaaacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat
    24781 tattggcgcg aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag
    24841 caaaccgtct gtgctggcac tgaacattca gcgcggcgac agctccatct acctgttaat
    24901 gcagtaatct ccctcaaccc cttcctgaaa acgggaaggg gttctcctta caatctgtga
    24961 acttcaccac aactccatac atcttcatca tcctttaggc atttgcacaa tgccgtacgt
    25021 tacgtacttc cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg
    25081 ataccaaaat ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca
    25141 acgtaatgga tgcccgtggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat
    25201 tgcacgaagg tgcattgctg gtactttcac agggacgagt cgtcgatatc gatgacgcgg
    25261 tggcacgtca tctgcacggt gtgcggcaag ggattaatct accgttacgg ctggaaggtg
    25321 aaattgtcgg cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac
    25381 tggtctgcat gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ttgttggctc
    25441 aggatagccg tttgcgggag gaactggtga tgaacctgat tcaggcagag gagaatactc
    25501 ccgcacttac tgaatgggcg caacggctgg ggatcgatct caatcaaccg cgagtggtgg
    25561 ctattgttga ggtcgacagc ggtcagcttg gtgtggacag cgcaatggcg gagttacaac
    25621 aactgcaaaa cgcgctgact acgcccgagc gtaataatct ggtggcgatt gtctcgctaa
    25681 ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg gcgctgggat gcagaagatc
    25741 atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga gtacggccag ctgcgttttc
    25801 gcgtttcact gggcaactat tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg
    25861 cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc tatttttatc
    25921 aggatctgat gttacctgtg ttactcgaca gtttgcgtgg cgactggcag gccaacgaac
    25981 tggcgcgact gctggcgcgg ctgaaagcga tggacaataa cggcttgctg cgacgaacgc
    26041 tggcggcgtg gtttcgtcac aatgtgcaac cgctggcaac gtcaaaggcg ttgtttattc
    26101 atcgtaatac tctggagtat cggcttaatc gtatatcgga actgaccgag cttgatttgg
    26161 gtaattttga tgacaggttg ctgctgtatg tggcgttgca gctggatgaa gagcggtagg
    26221 ttatgcgtta aggtcgaggc ggcgctggct cattcccctc agcccgaagg gtagagggga
    26281 atgtccgagt taaccgtcag ttcggcatta tttacgcgtt agtttttcca gatcggcttc
    26341 aatctcgctg atcttattgg ttacgactga ctccaggtga cgtaagtcgt cgaggatctt
    26401 acgcttaaga tcaacttcgc tgcggtcacg ctggcagatt tgatcaagct catcaatgat
    26461 ataccgtaga ttcgggctga tttcctggac ttctttataa ccctgaccca caccatcagc
    26521 gacgaccgtt ttacgctgac gcggatattt aaacttaacg ctcttggcga aaaactcgcc
    26581 tttgtctttc tggaaataga ttttcagaat atcgttgttg gcttcctgcc ggaggctgta
    26641 acgatcaatt tcttcaggat tggtaatacc cagacttttc agattgtcgt acatagcgtt
    26701 acctcaaaat gagtcagtaa attgtgctta ttttagcatt tggcctggcc cgcccggctc
    26761 gactgtgatc gcagaaagcc tttttcttcc agacttctat catggcgtaa aaatcaaaaa
    26821 attacctgct ttattgtggc gatattccat gggatggatt agcacattta tcttcgtttt
    26881 gcgaattaca tgacagtccg aaacaagatg aaaaagtatt taaaaaaggg gggaagaatg
    26941 agtaggtcga attattctga gccagaaagc atacgggcag cattatgctg cccgtatatg
    27001 tactcttaat cgatggtacg cagcagttcg ttaatgccga ctttgccgcg agttttcgcg
    27061 tcaactttct taacgataac cgcacagtag aggctgtatt tgccatcttt cgacggcaga
    27121 ttacctgaaa caaccacaga ccccgccgga acgcgaccgt agtggatttc gccggtttca
    27181 cggtcgtaaa tacgggtgct ctgaccaatg tatacgccca tagagatgac ggaaccttct
    27241 tcgacaatca ccccttcaac cacttcggag cgtgcgccaa tgaagcagtt atcttcaata
    27301 atggtcgggt tagcctgcag cggctccagc acgccgccga tgcccacgcc accggaaagg
    27361 tggacgtttt taccaatctg cgcacaagaa ccgacggtcg cccaggtatc aaccatcgtg
    27421 ccttcatcaa catatgcgcc gatgttgacg taagacggca tcagcacggt gttacgggca
    27481 ataaatgcac cctgacgtac cgccgctggt ggcacaacgc ggaagccttc tttctggaaa
    27541 cgtgcttcgt cgtagtcggc gaatttcatc ggcactttgt cgaagtagcg gctttctgcc
    27601 ccttcgatca cctgattatc attaatacgg aaagagagca gcaccgcttt tttcaaccac
    27661 tgatgcgtca cccactgacc gtcaattttt tccgctacac gcagtgcacc ggaatccagc
    27721 agggcgatca cctgatttac cgcttcgcgg gtaacggtgt ctgcgttggc tggcgtgatc
    27781 tcggcacggc gttcaaaagc ggtttcaata atgttctgta actgctgcat tgttaaactc
    27841 ttttcatatc agtaaacaca tcacccttta tcgtttggat tgagggcctc tgtcaaccgc
    27901 tgatgcactt cctgctgcaa ctcgttatta agtgcacgcc ggtcagcggt ggcaattatg
    27961 aataaatctt ctactcgctc gccaatggtt gtaattcggg caccatgaag cgaaattccc
    28021 agatcggcaa aaattttccc gactctcgcc agcagtcctg gctggtcgag cgcgataagc
    28081 tcaaggaacg atttgcggtc ggtatgggtc ggcaaaaacg ttacttcggt ttcaacagta
    28141 aaatggcgta atttggcggg ttggcgacgg ggctgcggtg gctgccagct actttgcgtc
    28201 agtacttgct ccagaccaaa ccgaataacc tcatgacgat ctgcggacag cgggctgcca
    28261 tcgggttcca gcacgataaa ggtatccatc gccataccgt cgcgagtggt gaaaatttgt
    28321 gcgtcgtgaa cacttaaatt gcggcggtct aattcggcac agacggcggc aaacagatag
    28381 gggcggtccg ggctccagat aaaaatctcg gtgcctccac gcgtagcctg cgggctaagc
    28441 aataccagcg gtttgcttaa atcatgctgt aataaatggc gggcatgcca ggccagttga
    28501 tttgggctat ggcggacaaa atagttagca cgacagcgtg accaaatttg gtgcagcgcc
    28561 tcttcgtcga tgttatccat gcgcagtagt gccagtgcct ggagttggtg atggcgaacc
    28621 cgttcgcgca tatccggcgt gttttgcatt ccgcgtcgta gctgcttttc ggtggcaaaa
    28681 tagagttcgc gcaacagact ttgcttccag ctattccaca gcgtttcgtt ggtggcgcaa
    28741 atgtcagcca ctgtcaggca taccagatag cgcagacgat tttccgtttg aacttcttcg
    28801 gcaaactgct tgatgacttc agggtcctga atatcgcggc gttgtgctgt caccgacatc
    28861 agcaagtgct ggcgaaccag ccaggcaacc agctgtgttt cgcgtgagtt cagcccgtgg
    28921 agttctgcaa aatgcactac atcctgagca ccgagaatgg agtggtcgcc gccgcgtcct
    28981 ttggcgatat cgtgaaacag cgcggcgatg aaaatcagct cagttgacgg caggcgcggc
    29041 cagacgtcca cacacaacgg atggcgctgg cgcgtttctt cactggcaaa actctccagt
    29101 ttcagcatca cgcggatagt atgttcatcc accgtgtagg cgtggaacag atcaaactgc
    29161 atctgcccga cgatatgcga ccattgcggc atatacgcgc cgagcacgct atggcgatgc
    29221 attggcaata gcccgcgccg caccgctccg gggtgacgca gaatgctcaa aaacagtttt
    29281 cgtgcttccg gaatattaca cagcggttgt tgcagatggc gacgggcatg gcgtaactgg
    29341 cgcagcgtgg tggagtaaat gccggtgatc gcactgttgc gcaccatggt gtagaacata
    29401 cgcaagatgg cttccggctg gcgcataaat agtgtttcat cacgcaggtc gattagcgta
    29461 ccgcgtagct gaaactcatc gtcgattgga cgtggttttt cgtcggcggg aagggcgagg
    29521 atggcttcat cgaacagttg cagcagcatc tggttgagtt cactgacgcg gcgtgtaacg
    29581 cggaagtaat ccttcatcat ccgctcgacc ggttcgttgc cttcaccact gtaattcaga
    29641 cgctgagcga cgctaagttg gcgatcgaat aacagacgat tatcgtaacg gctgacgacc
    29701 agatgcaggg caaagcgaat acgccacagt atatgcagac attcgtttaa ttccgcccgc
    29761 tccgctgagg ttaagaagcc aaacccaacc atttcatcca gcgatgttgc gccaaaatga
    29821 cggcgggcca cccattgcag agtgtggata tcgcgcaagc cgccagggct acttttgatg
    29881 tctggttcaa gattgtagct ggtgccgtgg taacgctgat ggcgctggtt ctgttcttca
    29941 actttcgccg cgtagaactt gtcggaaggc cagaatcctt cgctaaaaat atgtttttgc
    30001 agttcgagga agagcgcaac atcgccaatt aataagcggg attcgattaa attggtggcg
    30061 acggttaaat ccgataaccc ttccagcatg cactcttcaa gcgtgcgcac actatgaccg
    30121 acttccagct ttacatccca gagcagcgtt aacagctcgc ccactttttg cgcctgatcg
    30181 tccgggagct ttttacggct taaaatcagt aaatcgatgt cagaaagtgg atgcagctcg
    30241 ccacgaccgt agccaccgac ggcgaccaat gccaggtcgg caatctggct gaatcccgct
    30301 tcaatccata atcgttgcag gagctggtcg ataaactcgg tgcgcgcctc aatcaactgt
    30361 tctgcagaga tcccattgtc aaaggcatca cccagccaac gctggaaagt atcgatatgg
    30421 gcttttatcc caccgacggt taattcatca cggggccaga cgcatggatt ttgcggttga
    30481 ccgggcaggg tggggagagc ggtgtttgcg tactgttctg gaagggtatt cattgtgcgc
    30541 cacccataag attaaattat cgcattaaaa aagccggcat tcgccggctt catcttattc
    30601 gtcgtgcgag attatcgccg ggatggtgtc atccttgcgt agcgtcaaaa tttcgcagcc
    30661 gttatcagtc accacaatag tatgctcata ttgtgcagac aagctgcgat ctttggtttt
    30721 taccgtccag ccatctttca tggtgcggat ctctttttta cccgcgttga ccattggctc
    30781 aatggtgaac gtcatccccg gtttcagcac gacgttggtt tcacgggagt catagtgcag
    30841 cacctgcggt tcttcatgga agccgcgacc aataccgtgt ccacaatatt cacgaacgac
    30901 ggagaagcct tctgcttcga caaacttctg aatcgccgca ccgatttcgc gcaggttaat
    30961 gcctggtttg accatgcgca gcgccaggta caggctttct tgcgtgatgc ggcacagacg
    31021 ttcgcccatg atggtcggct taccgacgat aaacattttc gaggtatcgc cgtggaaacc
    31081 atctttgatt acggtgacat caatgttaac gatatcgcca tctttcagca gcttagcatc
    31141 gtccgggata ccgtggcaca ccacttcatt aatagagatg caaacggatt tcggatagcc
    31201 gtgatagccg aggcaggcag aaaccgcgtg ttgttcatta acaatgtaat cattacagat
    31261 gcgatccagc tcgccggtgc tgacgcccgg tttaacatac ggttcgatca tctccagcac
    31321 ttcggcagcc agtcggccag cgacgcgcat tttttcgata tcttctgggg tcttgattga
    31381 gatagccatt aattctgtcc atcagcgtcg gtgataccga caatatatat gtaagtgccg
    31441 tcaatggtat cacacccggg caaattgaga atcattctga atttcgccaa acgtgccact
    31501 gaaggttttc tataatagaa aattcgacat ctgatgctgt acacagcgcc aacaattatt
    31561 ggtgtccatg acgtatttgt ggtataaagc gcgccggact tccgatccat ttcgtataca
    31621 cagactggac ggaagcgaca atctcacttt gtgtaacaac acacacgtat cggcacatat
    31681 tccggggtgc cctttggggt cggtaatatg ggatacgtgg aggcataacc ccaactttta
    31741 tatagaggtt ttaatcatgg caactgtttc catgcgcgac atgctcaagg ctggtgttca
    31801 cttcggtcac cagacccgtt actggaaccc gaaaatgaag ccgttcatct tcggtgcgcg
    31861 taacaaagtt cacatcatca accttgagaa aactgtaccg atgttcaacg aagctctggc
    31921 tgaactgaac aagattgctt ctcgcaaagg taaaatcctt ttcgttggta ctaaacgcgc
    31981 tgcaagcgaa gcggtgaaag acgctgctct gagctgcgac cagttcttcg tgaaccatcg
    32041 ctggctgggc ggtatgctga ctaactggaa aaccgttcgt cagtccatca aacgtctgaa
    32101 agacctggaa actcagtctc aggacggtac tttcgaaaag ctgaccaaga aagaagcgct
    32161 gatgcgcact cgtgagctgg agaaactgga aaacagcctg ggcggtatca aagacatggg
    32221 cggtctgccg gacgctctgt ttgtaatcga tgctgaccac gaacacattg ctatcaaaga
    32281 agcaaacaac ctgggtattc cggtatttgc tatcgttgat accaactctg atccggacgg
    32341 tgttgacttc gttatcccgg gtaacgacga cgcaatccgt gctgtgaccc tgtacctggg
    32401 cgctgttgct gcaaccgtac gtgaaggccg ttctcaggat ctggcttccc aggcggaaga
    32461 aagcttcgta gaagctgagt aataaggctt gataactccc ccaaaatagt tcgagttgca
    32521 gaaaggcggc aagctcgaga attcccggga gcttacatca gtaagtgacc gggatgagcg
    32581 agcgaagata acgcatctgc ggcgcgaaat atgaaggggg agagccctta tagaccaggt
    32641 agtacacgtt tggttagggg gcctgcatat ggcccccttt ttcactttta tatctgtgcg
    32701 gtttaatgcc gggcagatca catctccgag gattttagaa tggctgaaat taccgcatcc
    32761 ctggtaaaag agctgcgtga gcgtactggc gcaggcatga tggattgcaa aaaagcactg
    32821 actgaagcta acggcgacat cgagctggca atcgaaaaca tgcgtaagtc cggtgctatt
    32881 aaagcagcga aaaaagcagg caacgttgct gctgacggcg tgatcaaaac caaaatcgac
    32941 ggcaactacg gcatcattct ggaagttaac tgccagactg acttcgttgc aaaagacgct
    33001 ggtttccagg cgttcgcaga caaagttctg gacgcagctg ttgctggcaa aatcactgac
    33061 gttgaagttc tgaaagcaca gttcgaagaa gaacgtgttg cgctggtagc gaaaattggt
    33121 gaaaacatca acattcgccg cgttgctgcg ctggaaggcg acgttctggg ttcttatcag
    33181 cacggtgcgc gtatcggcgt tctggttgct gctaaaggcg ctgacgaaga gctggttaaa
    33241 cacatcgcta tgcacgttgc tgcaagcaag ccagaattca tcaaaccaga agacgtatcc
    33301 gctgaagtgg tagaaaaaga ataccaggta cagctggata tcgcgatgca gtctggtaag
    33361 ccgaaagaaa tcgcagagaa aatggttgaa ggccgcatga agaaattcac cggcgaagtt
    33421 tctctgaccg gtcagccgtt cgttatggaa ccaagcaaaa ctgttggtca gctgctgaaa
    33481 gagcataacg ctgaagtgac tggcttcatc cgcttcgaag tgggtgaagg catcgagaaa
    33541 gttgagactg actttgcagc agaagttgct gcgatgtcca agcagtctta attatcgaaa
    33601 aggagccgcc tgagggcggc ttctttttgt gcccatcttg taaattcagc taacccttgt
    33661 ggggctgcgc tgaaaagcga cgtacaatgt cgctagtatt aattcatttc aatcgttgac
    33721 agtctcagga aagaaacatg gctaccaatg caaaacccgt ctataaacgc attctgctta
    33781 agttgagtgg cgaagctctg cagggcactg aaggcttcgg tattgatgca agcatactgg
    33841 atcgtatggc tcaggaaatc aaagaactgg ttgaactggg tattcaggtt ggtgtggtga
    33901 ttggtggggg taacctgttc cgtggcgctg gtctggcgaa agcgggtatg aaccgcgttg
    33961 tgggcgacca catggggatg ctggcgaccg taatgaacgg cctggcaatg cgtgatgcac
    34021 tgcaccgcgc ctatgtgaac gctcgtctga tgtccgctat tccattgaat ggcgtgtgcg
    34081 acagctacag ctgggcagaa gctatcagcc tgttgcgcaa caaccgtgtg gtgatcctct
    34141 ccgccggtac aggtaacccg ttctttacca ccgactcagc agcttgcctg cgtggtatcg
    34201 aaattgaagc cgatgtggtg ctgaaagcaa ccaaagttga cggcgtgttt accgctgatc
    34261 cggcgaaaga tccaaccgca accatgtacg agcaactgac ttacagcgaa gtgctggaaa
    34321 aagagctgaa agtcatggac ctggcggcct tcacgctggc tcgtgaccat aaattaccga
    34381 ttcgtgtttt caacatgaac aaaccgggtg cgctgcgccg tgtggtaatg ggtgaaaaag
    34441 aagggacttt aatcacggaa taattcccgt gatggataaa taagggtaag attccgcgta
    34501 agtatcgcgg gggcgttagt ctggttataa ggcgttattg ttgcaggcag tttggtcacg
    34561 gccagcgcgc agcaaccgga gcgtacaaaa gtacgtgagg atggcgagca ctgcccgggg
    34621 ccaaaatggc aaataaaata gcctaataat ccagacgatt acccgtaata tgtttaatca
    34681 gggctatact tagcacactt ccactgtgtg tgactgtctg gtctgactga gacaagtttt
    34741 caaggattcg taacgtgatt agcgatatca gaaaagatgc tgaagtacgc atggacaaat
    34801 gcgtagaagc gttcaaaacc caaatcagca aaatacgcac gggtcgtgct tctcccagcc
    34861 tgctggatgg cattgtcgtg gaatattacg gcacgccgac gccgctgcgt cagctggcaa
    34921 gcgtaacggt agaagattcc cgtacactga aaatcaacgt gtttgatcgt tcaatgtctc
    34981 cggccgttga aaaagcgatt atggcgtccg atcttggcct gaacccgaac tctgcgggta
    35041 gcgacatccg tgttccgctg ccgccgctga cggaagaacg tcgtaaagat ctgaccaaaa
    35101 tcgttcgtgg tgaagcagaa caagcgcgtg ttgcagtacg taacgtgcgt cgtgacgcga
    35161 acgacaaagt gaaagcactg ttgaaagata aagagatcag cgaagacgac gatcgccgtt
    35221 ctcaggacga tgtacagaaa ctgactgatg ctgcaatcaa gaaaattgaa gcggcgctgg
    35281 cagacaaaga agcagaactg atgcagttct gatttcttga acgacaaaaa cgccgctcag
    35341 tagatccttg cggatcggct ggcggcgttt tgctttttat tctgtctcaa ctctggatgt
    35401 ttcatgaagc aactcaccat tctgggctcg accggctcga ttggttgcag cacgctggac
    35461 gtggtgcgcc ataatcccga acacttccgc gtagttgcgc tggtggcagg caaaaatgtc
    35521 actcgcatgg tagaacagtg cctggaattc tctccccgct atgccgtaat ggacgatgaa
    35581 gcgagtgcga aacttcttaa aacgatgcta cagcaacagg gtagccgcac cgaagtctta
    35641 agtgggcaac aagccgcttg cgatatggca gcgcttgagg atgttgatca ggtgatggca
    35701 gccattgttg gcgctgctgg gctgttacct acgcttgctg cgatccgcgc gggtaaaacc
    35761 attttgctgg ccaataaaga atcactggtt acctgcggac gtctgtttat ggacgccgta
    35821 aagcagagca aagcgcaatt gttaccggtc gatagcgaac ataacgccat ttttcagagt
    35881 ttaccgcaac ctatccagca taatctggga tacgctgacc ttgagcaaaa tggcgtggtg
    35941 tccattttac ttaccgggtc tggtggccct ttccgtgaga cgccattgcg cgatttggca
    36001 acaatgacgc cggatcaagc ctgccgtcat ccgaactggt cgatggggcg taaaatttct
    36061 gtcgattcgg ctaccatgat gaacaaaggt ctggaataca ttgaagcgcg ttggctgttt
    36121 aacgccagcg ccagccagat ggaagtgctg attcacccgc agtcagtgat tcactcaatg
    36181 gtgcgctatc aggacggcag tgttctggcg cagctggggg aaccggatat gcgtacgcca
    36241 attgcccaca ccatggcatg gccgaatcgc gtgaactctg gcgtgaagcc gctcgatttt
    36301 tgcaaactaa gtgcgttgac atttgccgca ccggattatg atcgttatcc atgcctgaaa
    36361 ctggcgatgg aggcgttcga acaaggtcag gcagcgacga cagcattgaa tgccgcaaac
    36421 gaaatcaccg ttgctgcttt tcttgcgcaa caaatccgct ttacggatat cgctgcgttg
    36481 aatttatcgg tactggaaaa aatggatatg cgcgaaccac aatgtgtgga cgatgtgtta
    36541 tctgttgatg cgaacgcgcg tgaagtcgcc agaaaagagg tgatgcgtct cgcaagctga
    36601 ggataatccg gctacagaga gtcgcgctat ttgttagcgt agggcttcag tgatatagtc
    36661 tgcgccatct gatcgtaagt agttggcttt ataaggtcag gtatgccgtg gttttacacg
    36721 gctttttttt gtataggctt cagtattcct gagtaccgta aaccctgtca gggaataaaa
    36781 aacgcgtgat gttgtctgct actcaaccac ttagcgaaaa attgccagcg catggctgcc
    36841 gtcatgttgc gatcattatg gacggcaatg gtcgctgggc aaaaaagcaa gggaagattc
    36901 gtgcctttgg gcataaagcc ggggcaaaat ccgtccgccg ggctgtctct tttgcggcca
    36961 acaacggtat tgaggcgtta acgctgtatg cctttagtag tgaaaactgg aaccgaccag
    37021 cgcaggaagt cagtgcgtta atggaactgt ttgtgtgggc gctcgatagc gaagtaaaaa
    37081 gtctgcaccg acataacgtg cgtctgcgta ttattggcga taccagtcgc tttaactcgc
    37141 gtttgcaaga acgtattcgt aaatctgaag cgctaacagc cgggaatacc ggtctgacgc
    37201 tgaatattgc ggcgaactac ggtggacgtt gggatatagt ccagggagtc aggcaactgg
    37261 ctgaaaaggt gcagcaagga aacctgcaac cagatcagat agatgaagag atgctaaacc
    37321 agcatgtctg tatgcatgaa ctggcccctg tagatttagt aattaggact gggggggagc
    37381 atcgcattag taactttttg ctttggcaaa ttgcctatgc cgaactttac tttacagatg
    37441 ttctctggcc cgatttcgat gaacaagact ttgaaggggc gttaaatgcc tttgctaatc
    37501 gagagcgtcg tttcggcggc accgagcccg gtgatgaaac agcctgatgg gggtcgcttt
    37561 tgctgaagta tcgcctgata tctgcttttg tgttaatacc cgtcgtcatc gcggcgttgt
    37621 ttctgttgcc gccggtgggg ttcgccatcg taacgctggt ggtctgcatg ctggcagcgt
    37681 gggaatgggg acagcttagc ggttttacca ctcgttcgca gcgagtatgg ttggcggtgt
    37741 tatgcgggtt attgttggcg ctgatgcttt ttctgttgcc ggaatatcac cgaaatattc
    37801 atcaaccgct ggttgaaatc tcactttggg cttcgctggg ttggtggatt gtcgcgctat
    37861 tgctggtgct gttttaccca ggttccgcag caatctggcg taactctaaa acattgcgcc
    37921 ttatttttgg cgtgctaacc attgttccct tcttctgggg catgctggcg ttacgggcct
    37981 ggcactacga cgagaatcat tacagtggcg caatatggct gctctatgtc atgatcctgg
    38041 tatggggcgc tgactctggc gcatatatgt ttggcaaatt gtttggtaaa cataagctgg
    38101 caccgaaggt ttctccgggt aaaacctggc aaggctttat aggtggacta gcgaccgcag
    38161 cggtaatctc atggggttat ggcatgtggg cgaatctcga cgtcgctccc gtcaccttac
    38221 tcatttgctc tattgtcgca gcgttagcct cagtgctcgg cgatctgacc gagagtatgt
    38281 ttaagcgtga agcaggaatt aaggacagcg gtcatttaat tccaggacac ggtggtattt
    38341 tagatcgtat tgatagcctg acggctgcgg taccggtctt tgcttgcttg ttgttactgg
    38401 tattcaggac gctttaacgg aaggtaatat gctgagtttt ctctgggatt tggcttcgtt
    38461 catcgttgca ctgggtgtac ttatcaccgt gcatgaattt ggtcatttct gggttgcccg
    38521 gcgttgtggt gttcgcgttg agcgtttctc aatagggttt ggtaaggcgc tctggcggcg
    38581 aactgataag ctcggcaccg aatatgttat cgccctgatc ccgctgggcg gttatgtcaa
    38641 aatgctggat gagcgcgcag aaccggtcgt tccggaactc cgccaccatg ccttcaataa
    38701 taaatctgtt ggccaacgag cggcgattat tgccgcaggt ccggttgcaa acttcatttt
    38761 tgctatcttt gcctactggc tggtttttat tattggtgtg cctggcgtac gtccggtggt
    38821 tggtgaaata gcagccaatt cgatagctgc ggaagcacaa attgcaccag gtacggaact
    38881 aaaagccgta gatggtatcg aaacgcctga ttgggatgcc gtgcgtttgc agttggtcga
    38941 taaaattggc gatgaaagca ccaccattac agtagcgcca tttggcagcg atcaacggcg
    39001 ggatgtaaag ctcgatttac gtcactgggc gtttgagcct gataaagaag atccggtatc
    39061 ttcgctgggg attcgtcctc gtgggccgca aattgaacct gtactggaaa atgtgcagcc
    39121 aaactcggcg gcaagcaagg caggtttgca agcaggcgac aggatcgtta aagtcgatgg
    39181 tcagccctta acgcagtggg tgacctttgt gatgcttgtc cgggataacc cgggtaaatc
    39241 cttagcgtta gaaatcgaaa ggcagggaag tcccttgtct ttgacattaa tcccggagag
    39301 taaaccgggt aatggtaaag cgattggttt tgtcggtatt gagccgaaag tcattccttt
    39361 gccagatgag tataaagttg tacgccagta tgggccgttc aacgccatcg tcgaagccac
    39421 ggacaaaacg tggcagctga tgaagctgac ggtcagtatg ctgggaaaat tgatcaccgg
    39481 tgatgtgaaa ctgaacaacc tcagtgggcc gatctctatc gccaaggggg ctgggatgac
    39541 agcggaactc ggggtagttt attacctgcc gtttcttgcg cttattagcg tgaacttagg
    39601 gataattaac ctgtttccgt tgcccgtact tgacgggggg catctgctgt tccttgcgat
    39661 cgaaaagatc aagggcggac cggtatccga gcgggttcaa gacttttgtt atcgcattgg
    39721 ctcgattctg ctggtgctgt taatggggct tgcacttttc aatgatttct ctcggttatg
    39781 agagttagtt aggaagaacg cataataacg atggcgatga aaaagttgct catagcgtcg
    39841 ctgctgttta gcagcgccac cgtatacggt gctgaagggt tcgtagtgaa agatattcat
    39901 ttcgaaggcc ttcagcgtgt cgccgttggt gcggccctcc tcagtatgcc ggtgcgcaca
    39961 ggcgacacgg ttaatgatga agatatcagt aataccattc gcgctctgtt tgctaccggc
    40021 aactttgagg atgttcgcgt ccttcgtgat ggtgataccc ttctggttca ggtaaaagaa
    40081 cgtccgacca ttgccagcat tactttctcc ggtaacaaat cggtgaaaga tgacatgctg
    40141 aagcaaaacc tcgaggcttc tggtgtgcgt gtgggcgaat ccctcgatcg caccaccatt
    40201 gccgatatcg agaaaggtct ggaagacttc tactacagcg tcggtaaata tagcgccagc
    40261 gtaaaagctg tcgtgacccc gctgccgcgc aaccgtgttg acctaaaact ggtgttccag
    40321 gaaggtgtgt cagctgaaat ccagcaaatt aacattgttg gtaaccatgc tttcaccacc
    40381 gacgaactga tctctcattt ccaactgcgt gacgaagtgc cgtggtggaa cgtggtaggc
    40441 gatcgtaaat accagaaaca gaaactggcg ggcgaccttg aaaccctgcg cagctactat
    40501 ctggatcgcg gttatgcccg tttcaacatc gactctaccc aggtcagtct gacgccagat
    40561 aaaaaaggta tttacgtcac ggtgaacatc accgaaggcg atcagtacaa gctttctggc
    40621 gttgaagtga gcggcaacct tgccgggcac tccgctgaaa ttgagcagct gactaagatc
    40681 gagccgggtg agctgtataa cggcaccaaa gtgaccaaga tggaagatga catcaaaaag
    40741 cttctcggtc gctatggtta tgcctatccg cgcgtacagt cgatgcccga aattaacgat
    40801 gccgacaaaa ccgttaaatt acgtgtgaac gttgatgcgg gtaaccgttt ctacgtgcgt
    40861 aagatccgtt ttgaaggtaa cgatacctcg aaagatgccg tcctgcgtcg cgaaatgcgt
    40921 cagatggaag gtgcatggct ggggagcgat ctggtcgatc agggtaagga gcgtctgaat
    40981 cgtctgggct tctttgaaac tgtcgatacc gatacccaac gtgttccggg tagcccggac
    41041 caggtagatg tagtctacaa ggtgaaagaa cgtaacaccg gtagcttcaa cttcgggatt
    41101 ggttacggta ctgaaagtgg cgtgagcttc caggctggtg tgcagcagga taactggtta
    41161 ggtacaggtt atgctgttgg tatcaacggg accaaaaacg attaccagac ctatgctgaa
    41221 ctgtcggtaa ccaacccgta cttcactgta gatggtgtaa gcctcggtgg tcgtctcttc
    41281 tataatgact tccaggcaga tgacgccgac ctgtccgact ataccaacaa gagttatggt
    41341 acagacgtga cgttgggctt cccgatcaac gaatataact cgctgcgtgc aggtctgggt
    41401 tatgtacata actccctgtc caacatgcag cctcaggttg cgatgtggcg ttatctgtac
    41461 tctatgggtg aacatccgag cacctctgat caggataata gcttcaaaac ggacgacttc
    41521 acgttcaact atggttggac ctataacaag cttgaccgtg gttacttccc gacagatggt
    41581 tcacgtgtca acctgaccgg taaagtgacc attcctggat cggataacga atactacaaa
    41641 gtgacgttag acacggcgac ttatgtgccg atcgatgacg atcacaaatg ggttgttctg
    41701 gggcgtaccc gctggggtta tggtgatggt ttaggcggca aagagatgcc gttctacgag
    41761 aacttctatg ccggtggttc cagcaccgtg cgtggcttcc agtccaatac aattggtccg
    41821 aaagcagttt acttcccgca tcaggccagt aattatgatc cggactatga ttacgaatgt
    41881 gcgactcagg acggcgcgaa agacctgtgt aaatcggatg atgctgtagg cggtaacgcc
    41941 atggcggttg ccagcctcga gttcatcacc ccgacgccgt ttattagcga taagtatgct
    42001 aactcggttc gtacttcctt cttctgggat atgggtaccg tttgggatac aaactgggat
    42061 tccagccaat attctggata tccggactat agtgatccaa gcaatatccg tatgtctgcg
    42121 ggtatcgcat tacaatggat gtccccattg gggccgttgg tgttctccta cgcccagccg
    42181 ttcaaaaagt acgatggaga caaggcagaa cagttccagt ttaacatcgg taaaacctgg
    42241 taagtgttct ccacaaagga atgtagtggt agtgtagcga tgactttagg cgatcaatat
    42301 aagatcgccg ggccacgcaa agaactgcac cctccggtgc aaatgggatg gtaaggagtt
    42361 tattgtgaaa aagtggttat tagctgcagg tctcggttta gcactggcaa cttctgctca
    42421 ggcggctgac aaaattgcaa tcgtcaacat gggcagcctg ttccagcagg tagcgcagaa
    42481 aaccggtgtt tctaacacgc tggaaaatga gttcaaaggc cgtgccagcg aactgcagcg
    42541 tatggaaacc gatctgcagg ctaaaatgaa aaagctgcag tccatgaaag cgggcagcga
    42601 tcgcactaag ctggaaaaag acgtgatggc tcagcgccag acttttgctc agaaagcgca
    42661 ggcttttgag caggatcgcg cacgtcgttc caacgaagaa cgcggcaaac tggttactcg
    42721 tatccagact gctgtgaaat ccgttgccaa cagccaggat atcgatctgg ttgttgatgc
    42781 aaacgccgtt gcttacaaca gcagcgatgt aaaagacatc actgccgacg tactgaaaca
    42841 ggttaaataa gtaatgcctt caattcgact ggctgattta gcgcagcagt tggatgcaga
    42901 actacacggt gatggcgata tcgtcatcac cggcgttgcg tccatgcaat ctgcacaaac
    42961 aggtcacatt acgttcatgg ttaacccaaa ataccgtgag catttaggct tgtgccaggc
    43021 gtccgcggtt gtcatgaccc aggacgatct tcctttcgcg aaaagtgccg cgctggtagt
    43081 gaagaatccc tacctgactt acgcgcgcat ggcgcaaatt ttagatacca cgccgcagcc
    43141 cgcgcagaac attgcaccca gtgcggtgat cgacgcgacg gcaaagctgg gtaacaacgt
    43201 atcgattggc gctaacgcgg tgattgagtc cggcgttgaa ctgggcgata acgtgattat
    43261 cggtgccggt tgcttcgtag gtaaaaacag caaaatcggt gcaggttcgc gtctctgggc
    43321 gaacgtaacc atttaccatg agatccagat cggtcagaat tgcctgatcc agtccggaac
    43381 agtggtaggc gcagacggct ttggttatgc caacgatcgt ggtaactggg tgaagatccc
    43441 acagattggt cgcgtaatta ttggcgatcg cgtggagatc ggtgcctgca caaccatcga
    43501 tcgcggcgcg ctggatgaca ctgttattgg caatggcgtg atcattgata accagtgcca
    43561 gattgcacat aacgtcgtga ttggcgacaa tacggcggtt gccggtggcg tcattatggc
    43621 gggcagcctg aaaattggtc gttactgcat gatcggcgga gccagcgtaa tcaacgggca
    43681 tatggaaata tgcgacaaag tgacggttac gggcatgggt atggtgatgc gtcccatcac
    43741 tgaaccaggc gtctattcct caggcattcc gctgcaaccc aacaaagtct ggcgcaaaac
    43801 cgctgcactg gtgatgaaca ttgatgacat gagcaagcgt ctgaaatcgc ttgagcgcaa
    43861 ggttaatcaa caagactaac gttccatctt ttgttcgcca aactttacgg cctgtctcat
    43921 tcttacgatt gcggcaggcc gtgttattat tgtcgtttct tatattttga caggaagagt
    43981 atcttgacta ctaacactca tactctgcag attgaagaga ttttagaact tctgccgcac
    44041 cgtttcccgt tcttactggt ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc
    44101 gcagtaaaaa atgtctctgt caatgagcca ttcttccagg gccatttccc tggaaaaccg
    44161 attttcccgg gtgtgctgat tctggaagca atggcacagg caacaggtat tctggcgttt
    44221 aaaagcgtag gaaaactgga accgggtgag ctgtactact tcgctggtat tgacgaagcg
    44281 cgcttcaagc gcccggtcgt gcctggcgat caaatgatca tggaagtcac tttcgaaaaa
    44341 acgcgccgcg gcctgacccg ttttaaaggg gttgctctgg tcgatggtaa agtagtttgc
    44401 gaagcaacga tgatgtgtgc tcgtagccgg gaggcctgat aagtgattga taaatccgcc
    44461 tttgtgcatc caaccgccat tgtggaagag ggcgcgtcaa ttggcgcgaa cgcacacatt
    44521 ggtccttttt gtatcgttgg accccatgtc gaaattggtg agggtaccgt actgaaatct
    44581 cacgttgtcg tgaatggtca tactaaaatt ggccgcgata atgagattta tcagttcgcc
    44641 tccatcggcg aagttaacca ggatctgaaa tatgctggcg aaccgacccg tgtggaaatc
    44701 ggcgatcgta accgcattcg cgaaagcgtc accattcatc gtggcacagt ccagggcggt
    44761 ggattgacga aggtgggcag cgacaactta ctgatgatca acgcgcacat tgcgcacgat
    44821 tgtacggtag gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg
    44881 gttgacgact tcgcgatcat cggcggcatg accgcagtcc atcagttctg catcattggt
    44941 gcgcacgtga tggttggcgg ctgctccggt gtggcgcagg acgtccctcc ttatgtcatt
    45001 gcgcagggta accacgcaac gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga
    45061 ttcagccgtg aggcgattac cgctatccgc aatgcgtata agctgattta tcgtagcggt
    45121 aaaacgctcg atgaagtgaa accggaaatt gctgaactgg cggaaacata tccggaagtg
    45181 aaagccttta ccgatttctt tgcacgctca acgcgcggtc tgattcgtta atgactgaac
    45241 agcgtccatt aacgattgcc ctggtcgccg gagaaacctc cggcgatatc ctgggggccg
    45301 gtttaatccg cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc
    45361 cacgaatgca ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg
    45421 gcattgttga agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa
    45481 agcgttttgg cgaactgaag ccagatattt ttgttggtat tgatgcgcct gacttcaata
    45541 ttactcttga aggtaacctc aaaaagcagg gtatcaaaac cattcattac gtcagtccat
    45601 ccgtctgggc gtggcgacag aaacgcgttt tcaaaatagg cagagccacc gatctggtgc
    45661 tcgcatttct gcctttcgaa aaagcgtttt atgacaaata caatgtaccg tgccgcttta
    45721 tcggtcatac catggctgat gccatgccgt tagatccaga taaaaatgcc gcccgtgatg
    45781 tgctggggat ccctcacgat gcccactgcc tggcgttgct accagggagc cgtggtgcag
    45841 aagttgaaat gcttagtgcc gatttcctga aaacggccca gcttttgcgc cagacatatc
    45901 cggatctcga aatcgtggtg ccactggtga atgccaaacg ccgcgagcag tttgaacgca
    45961 tcaaagctga agtcgcgcca gacctttcag ttcatttgct ggatgggatg ggccgtgagg
    46021 cgatggtcgc cagcgatgcg gcgctactgg cgtcgggtac ggcagccctg gagtgtatgc
    46081 tggcgaaatg cccgatggtg gtgggatatc gcatgaagcc ttttaccttc tggttggcga
    46141 agcggctggt gaaaactgat tatgtctcgc tgccaaatct gctggcgggc agagagttag
    46201 tcaaagaatt attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc
    46261 tgttggcgaa cgggaaaacc agccacgcga tgcacgatac cttccgtgaa ctgcatcagc
    46321 agatccgctg caatgccgat gagcaggcgg cacaagccgt tctggagtta gcacaatgat
    46381 cgaatttgtt tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg gacgtgggcc
    46441 gttagttggc gcggtcgtca ccgctgcggt gatccttgac ccggcgcgcc cgattgccgg
    46501 gctgaatgat tccaaaaagc tgagcgaaaa acgccgtctg gcgctctatg aagagatcaa
    46561 agagaaagcg ttgagctgga gtctgggccg cgcggaaccc cacgaaatcg acgagctgaa
    46621 cattcttcat gcgaccatgc tggcgatgca gcgtgccgtc gctgggctgc atattgcgcc
    46681 ggaatatgtg ttgattgatg gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt
    46741 ggtgaaaggc gatagccgcg taccggaaat cagtgccgcg tctatcctgg cgaaagtgac
    46801 gcgtgacgcc gaaatggcgg cgctggatat tgttttcccg caatatggtt ttgcccaaca
    46861 caaagggtac ccaaccgctt ttcatctgga aaaactggct gaacacggcg cgaccgaaca
    46921 ccatcggcgc agctttgggc ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg
    46981 tcgagattaa gtaaaccgga atctgaagat gtctgaacca cgtttcgtac acctgcgggt
    47041 gcacagcgac tactcgatga tcgatggctt ggccaaaacc gcaccgttgg taaaaaaggc
    47101 ggcggcgttg ggtatgccag cactggcgat caccgatttc accaaccttt gtggtctggt
    47161 gaagttctac ggagcgggac atggcgcagg gattaagcct atcgtcgggg cagattttaa
    47221 cgtccagtgc gacctgctgg gtgatgagtt aacccacctg acggtactgg cggcgaacaa
    47281 taccggctat cagaatctga cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc
    47341 cgccgggccg atcatcgatc gcgactggct tatcgaatta aacgaagggt tgatccttct
    47401 ttccggcgga cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt
    47461 agatgagtgt gtcgcgtttt atgaagaaca cttcccggat cgctattttc ttgagctgat
    47521 ccgcaccggc aggccggatg aagaaagcta tctgcacgcg gcggtggaac tggcggaagc
    47581 gcgcggtttg cccgtcgtgg cgaccaacga cgtgcgcttt atcgacagca gcgactttga
    47641 cgcacacgaa atccgcgtcg cgatccacga cggctttacc ctcgacgatc ctaaacgccc
    47701 gcgtaactat tcgccgcagc aatatatgcg tagcgaagag gagatgtgtg agctgtttgc
    47761 cgacatcccc gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta acgtaaccgt
    47821 gcgtcttggt gaatacttcc tgccgcagtt cccgaccggg gacatgagca ccgaagatta
    47881 tctggtcaag cgtgcaaaag agggcctgga agagcgtctg gcctttttat tccctgatga
    47941 ggaagaacgt cttaagcgcc gcccggaata tgacgaacgt ctggagactg aacttcaggt
    48001 tatcaaccag atgggcttcc cgggctactt cctcatcgtt atggaattta tccagtggtc
    48061 gaaagataac ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt cactggtggc
    48121 ctacgcgctg aaaatcaccg acctcgatcc gctggaattt gacctgctgt tcgaacgttt
    48181 ccttaacccg gaacgtgtct ccatgcctga cttcgacgtt gacttctgta tggagaaacg
    48241 cgatcaggtt atcgagcacg tagcggacat gtacggtcgt gatgcggtat cgcagattat
    48301 caccttcggt acgatggcgg cgaaagcggt gatccgcgac gtaggccgcg tgctggggca
    48361 tccgtacggc tttgtcgatc gtatctcgaa actgatcccg cccgatccgg ggatgacgct
    48421 ggcgaaagcg tttgaagccg agccgcagct gccggaaatc tacgaagcgg atgaagaagt
    48481 taaggcgctg atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca
    48541 cgccggtggg gtggttatcg caccgaccaa aattaccgat tttgcgccgc tttattgcga
    48601 tgaagagggc aaacatccgg tcacccagtt tgataaaagc gacgttgaat acgccggact
    48661 ggtgaagttc gacttccttg gtttgcgtac gctcaccatc atcaactggg cgctggagat
    48721 gatcaacaag cggcgggcga agaatggcga gccgccgctg gatatcgccg cgatcccgct
    48781 ggatgacaag aaaagcttcg acatgctgca acgctcggaa accactgcgg tattccagct
    48841 tgaatcgcgc ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct tcgaagatat
    48901 gatcgccctg gtggcactgt tccgccccgg tccgttgcaa tcagggatgg tggataactt
    48961 tatcgaccgt aaacatggtc gtgaagagat ctcctatccg gacgtgcagt ggcagcatga
    49021 aagcttgaaa ccggtactgg agccaaccta cggcatcatc ctgtatcagg aacaggtcat
    49081 gcagattgcc caggtgcttt ctggttatac cctcggtggc gcggatatgc tgcgtcgtgc
    49141 gatggggaag aaaaagccgg aagagatggc taagcaacgt tctgtatttg ctgaaggtgc
    49201 agaaaagaac ggaatcaacg ctgaactggc gatgaaaatc ttcgacctgg tggagaaatt
    49261 cgctggttac ggatttaaca aatcgcactc tgcggcctat gctttggtgt catatcaaac
    49321 gttatggctg aaagcacact atcctgcgga gtttatggcg gcggtaatga ccgccgatat
    49381 ggacaacacc gagaaggtgg tgggtctggt ggatgagtgc tggcggatgg ggctgaaaat
    49441 cctgccacca gatataaact ccggtcttta ccatttccac gtcaacgacg acggcgaaat
    49501 cgtgtatggt attggcgcga tcaaaggggt cggtgaaggt ccgattgagg ccatcatcga
    49561 agcccgtaat aaaggcggct acttccgcga actgtttgat ctctgcgccc gtaccgacac
    49621 caaaaagttg aaccgtcgcg tgctggaaaa actgatcatg tccggggcgt ttgaccgtct
    49681 tgggccacat cgcgcggcgc tgatgaactc gctgggtgat gcgttaaaag cggcagatca
    49741 acacgcgaaa gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc
    49801 ggaacaaatt gaacaatcct acgccagctg ccaaccgtgg ccggagcagg tggtattaga
    49861 tggggaacgt gaaacgttag gcctgtacct gaccggacac cctatcaacc agtatttaaa
    49921 agagattgag cgttatgtcg gaggcgtaag gctgaaagac atgcacccga cagaacgtgg
    49981 taaagtcatc acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg
    50041 caatcgtatc ggtatctgca cgctggatga ccgttccggg cggctggaag tgatgttgtt
    50101 tactgacgcc ctggataaat accagcaatt gctggaaaaa gaccgcatac ttatcgtcag
    50161 cggacaggtc agctttgatg acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat
    50221 ggatattgac gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc tgacggacag
    50281 gcaaattgat gaccagcttt taaaccgact ccgtcagtct ctggaacccc accgctctgg
    50341 gacaattcca gtacatctct actatcagag ggcggatgca cgcgcgcggt tgcgttttgg
    50401 cgcgacgtgg cgtgtctctc cgagcgatcg tttattaaac gatctccgtg gcctcattgg
    50461 ttcggagcag gtggaactgg agtttgacta atacaggaat actatgagtc tgaatttcct
    50521 tgattttgaa cagccgattg cagagctgga agcgaaaatc gattctctga ctgcggttag
    50581 ccgtcaggat gagaaactgg atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa
    50641 aagcgtagaa ctgacacgta aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact
    50701 ggcacgccat ccacagcgtc cttataccct ggattacgtt cgcctggcat ttgatgaatt
    50761 tgacgaactg gctggcgacc gcgcgtatgc agacgataaa gctatcgtcg gtggtatcgc
    50821 ccgtcttgac ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga
    50881 aaaaattcgc cgtaactttg gtatgccagc gccagaaggt taccgcaaag cactgcgtct
    50941 gatgcaaatg gctgaacgct ttaagatgcc aatcatcacc tttatcgaca ccccgggggc
    51001 ttatccgggc gtgggcgcag aagagcgcgg tcagtctgaa gccattgcac gcaacctgcg
    51061 tgaaatgtct cgccttagcg taccgactat ttgtaccgtt atcggtgaag gtggttctgg
    51121 cggcgcgctg gcgattggcg tgggcgataa agtgaatatg ctgcaataca gcacctattc
    51181 cgttatctcg ccggaaggtt gtgcgtccat tctgtggaag agcgctgata aagcgccgct
    51241 ggcggctgaa gcgatgggta tcatcgctcc gcgtctgaaa gaactaaaac tgatcgactc
    51301 catcatcccg gaaccgctgg gtggtgctca ccgtaacccg gaagcgatgg cggcatcgtt
    51361 gaaagcgcaa ctgctggcgg atctggccga tctcgatgtg ttaagcactg aagatttaaa
    51421 aaatcgtcgt tatcagcgcc tgatgagcta cggttacgcg taattcgcaa aagttctgaa
    51481 aaagggtcac ttcggtggcc cttttttatc gccacggttt gagcaggcta tgattaagga
    51541 aggattttcc aggaggaaca catgaacatc attgccatta tgggaccgca tggcgtcttt
    51601 tataaagatg agcccatcaa agaactggag tcggcgctgg tggcgcaagg ctttcagatt
    51661 atctggccac aaaacagcgt tgatttgctg aaatttatcg aacataaccc tcgaatttgc
    51721 ggcgtgattt ttgactggga tgagtacagt ctcgatttat gcagcgatat caatcaactt
    51781 aatgaatatc tcccgcttta tgccttcatc aacacccact cgacgatgga tgtcagcgtg
    51841 caggatatgc ggatggcgct ctggtttttt gaatatgcgc tggggcaggc ggaagatatc
    51901 gccattcgta tgcgtcagta caccgacgaa tatcttgata acattacacc gccgttcacg
    51961 aaagccttgt ttacctacgt caaagagcgg aagtacacct tttgtacgcc ggggcatatg
    52021 ggcggcaccg catatcaaaa aagcccggtt ggctgcctgt tttatgattt tttcggcggg
    52081 aataccctca aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt gctcgaccac
    52141 accgggccac acctggaagc ggaagagtac atcgcgcgga cttttggcgc ggaacagagt
    52201 tatatcgtta ccaacggaac atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca
    52261 tccggcagta cgctgttgat cgaccgcaat tgtcataaat cgctggcgca tctgttgatg
    52321 atgaacgatg tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg gattcttggt
    52381 gggatcccgc gccgtgaatt tactcgcgac agcatcgaag agaaagtcgc tgccaccacg
    52441 caggcacaat ggccggttca tgcggtgatt accaactcca cctatgatgg cttgctctac
    52501 aacaccgact ggatcaaaca gacgctggat gtcccgtcga tccacttcga ttccgcctgg
    52561 gtgccgtaca cccattttca tccgatctac caggggaaaa gtggtatgag cggcgagcgt
    52621 gttgcgggaa aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg
    52681 caggcttcgc tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt
    52741 atgatgcata ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg
    52801 gcgatgctgc gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg
    52861 cattttcgca aagaggtcca gcggttgcgg gaagagtctg acggttggtt tttcgatatc
    52921 tggcaaccgc cgcaggtgga tgaagccgaa tgctggcccg ttgcgccagg cgaacagtgg
    52981 cacggcttta acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt
    53041 ttgacaccgg ggatggacga acagggcaat atgagcgagg aggggatccc ggcggcgctg
    53101 gtggcaaaat tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg
    53161 ctgtttctct ttagtattgg tatcgataaa accaaagcaa tgggattatt gcgtgggttg
    53221 acggaattta aacgctctta cgatctcaac ctgcggatca aaaatatgct acccgatctc
    53281 tatgctgaag atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc
    53341 cataagctga ttcgtaaaca cgatcttccc ggtttgatgt tgcgggcatt cgatactttg
    53401 ccggaaatga tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa
    53461 accattgcgc tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca
    53521 ccgggcgtac cgctgttgat gccaggagaa atgctgacca aagagagccg cacggtactc
    53581 gattttctac tgatgctttg ttccgtagtg caacattacc ccggttttga aacggatatt
    53641 cacggcgcga aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga
    53701 taacttgcca gagcggcttc cgggcgagta acgtgctgtt aacaaataaa ggagacgtta
    53761 tgctgggttt aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca
    53821 aagctttcta ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc
    53881 gcgactcgtg gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt
    53941 ttccgttccc gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt
    54001 ttagcgttga tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg
    54061 aagccatccg tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg
    54121 ggctgccgtt ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg
    54181 tgccgggcat tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt
    54241 ctcacctcac gccagattct ggtggccttt agcggcgggc ttgactccac cgttctgctg
    54301 catcagttgg tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg
    54361 catcacggtt taagtgccaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa
    54421 cagtggcagg tgccgctggt ggtcgaacgc gtgcaacttg cgcaagaagg actgggcatt
    54481 gaggcccagg cgcggcaggc gcgttatcag gcatttgccc gcaccttgtt gcccggtgaa
    54541 gtgctggtca ccgcgcaaca tctcgacgat caatgtgaaa cttttctgct ggcgctaaaa
    54601 cgcggtagcg gccctgccgg gctttcggct atggcggaag tttcggagtt tgccggaaca
    54661 cggcttatcc gcccgttgct cgcccgcacg cggggagaac tggagcagtg ggcattggcg
    54721 catggtttac gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt
    54781 ctgcgcctgc gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg
    54841 gcccgcagcg ccgcactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat
    54901 gatttagcac actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg
    54961 agtgatgccc gccgcgcggc gattatccgt cgctggctgg cagggcagaa tgcaccaatg
    55021 ccttcccgcg acgcgctggt gcggatctgg caggaagtgg cgctggcgcg ggaagatgcc
    55081 tccccctgtt tacgcttggg cgcgtttgaa atccgccgct atcagtcgca actgtggtgg
    55141 attaaatccg tcaccgggca aagtgaaacc attgtgccgt ggcagacgtg gctacaaccg
    55201 ctggaattac cggcggggct gggaagcgta cagcttactg cgggaggcga tattcgccct
    55261 ccgcgtgcag acgaagcggt cagcgtgcgt ttcaaagcgg caggattgct gcatattgtc
    55321 gggcgcaacg gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg
    55381 ctacgtgaca ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggta
    55441 tttgtgacgc aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa
    55501 acgcttagtt aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagttaa
    55561 tcaggactcg ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg taattttatc
    55621 cagacgtagc tcacgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg
    55681 ggagactaaa tcgctggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag
    55741 cattaaatga tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg
    55801 atacgtatca ttcattgaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt
    55861 tttccctgac agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa
    55921 cgcagcccag cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct
    55981 tgcgtattaa ctcacaatat ttttccacat gccctcctgt cagcactctg acttaaccgt
    56041 ggatgcaagt ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc
    56101 atttctgcaa tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga
    56161 aaggagtttt cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga
    56221 tctcgaccgc aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac
    56281 ccaggagcgt atgatgctgc gcttgctggc gtggctgaaa tatgctgatg aacgtctgca
    56341 atttacccgt ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct
    56401 gggcattgat ttgtggattg agctggggct gccggatgag cggcggatta agaaagcctg
    56461 cacccaggcc gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg
    56521 gcagcaaaat cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga
    56581 tgaacaactg gcgaaagtaa gcgtctttgc cgatcgtacc atgacgctgc aggcaacgat
    56641 tcaggatggc gtgatctggt tatcggacga taagaataat ctggaagtta acttaaccgc
    56701 ctggcaacaa ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt
    56761 gagatcaccg ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa gacctcaacg
    56821 gccattcatc tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcgt
    56881 ctactcgccg ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag
    56941 gaataccgca gtcaggaact gaaccgcgaa gccgcgctag cccggctggt ggcagtgatt
    57001 aaagatttaa caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa
    57061 gagcgcaggc tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta
    57121 cgcagcggtc gggaataaaa agaaggaata gatggtgaaa aaagcgatag tgacagcgat
    57181 ggctgtaatc agcctcttta ctctgatggg atgtaataac cgggccgaag tcgatacgct
    57241 ttctccagca caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctgcc
    57301 gtgtgctgat tgcgaaggaa tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt
    57361 gatgaatgag cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac
    57421 atgggcgcga accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta
    57481 tcgggcgaaa ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca
    57541 gttcaactat acgctggaac cggcacaatc tagtttacct atgacgccga tgaccctgcg
    57601 aggcatgtat ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg
    57661 tttcatggta gcgaataacg cagagctgga gcgtagctac ctggctgcgc gcggtcacag
    57721 tgaaaaaccg gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac
    57781 tggtgcgcca accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga
    57841 ttgcagtagt ttggggcagt aaacctgctt aaggcagaaa caaacg
//
LOCUS       DAZFYC010000013        57813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFYC010000013 DAZFYC010000000
VERSION     DAZFYC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.013"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            275..1333
                     /gene="citC"
                     /locus_tag="ACHKW5_000976"
     CDS             275..1333
                     /gene="citC"
                     /locus_tag="ACHKW5_000976"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO7510413.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1348..1644
                     /gene="citD"
                     /locus_tag="ACHKW5_000977"
     CDS             1348..1644
                     /gene="citD"
                     /locus_tag="ACHKW5_000977"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO7510414.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1641..2549
                     /gene="citE"
                     /locus_tag="ACHKW5_000978"
     CDS             1641..2549
                     /gene="citE"
                     /locus_tag="ACHKW5_000978"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO7510415.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2560..4092
                     /gene="citF"
                     /locus_tag="ACHKW5_000979"
     CDS             2560..4092
                     /gene="citF"
                     /locus_tag="ACHKW5_000979"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO7510416.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4096..4647
                     /gene="citX"
                     /locus_tag="ACHKW5_000980"
     CDS             4096..4647
                     /gene="citX"
                     /locus_tag="ACHKW5_000980"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO7510417.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4622..5500
                     /gene="citG"
                     /locus_tag="ACHKW5_000981"
     CDS             4622..5500
                     /gene="citG"
                     /locus_tag="ACHKW5_000981"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO7510418.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5551..7014
                     /gene="citT"
                     /locus_tag="ACHKW5_000982"
     CDS             5551..7014
                     /gene="citT"
                     /locus_tag="ACHKW5_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO7510419.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGGILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7128..7934
                     /gene="rna"
                     /locus_tag="ACHKW5_000983"
     CDS             7128..7934
                     /gene="rna"
                     /locus_tag="ACHKW5_000983"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO7510420.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8164..8574
                     /gene="rnk"
                     /locus_tag="ACHKW5_000984"
     CDS             8164..8574
                     /gene="rnk"
                     /locus_tag="ACHKW5_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO7510421.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLRIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8712..8801
                     /gene="yldA"
                     /locus_tag="ACHKW5_000985"
     CDS             8712..8801
                     /gene="yldA"
                     /locus_tag="ACHKW5_000985"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO7510422.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8805..10043)
                     /gene="ybdR"
                     /locus_tag="ACHKW5_000986"
                     /pseudo
     CDS             complement(8805..10043)
                     /gene="ybdR"
                     /locus_tag="ACHKW5_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10264..10692
                     /gene="uspG"
                     /locus_tag="ACHKW5_000987"
     CDS             10264..10692
                     /gene="uspG"
                     /locus_tag="ACHKW5_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO7510423.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10813..12378)
                     /gene="ahpF"
                     /locus_tag="ACHKW5_000988"
     CDS             complement(10813..12378)
                     /gene="ahpF"
                     /locus_tag="ACHKW5_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO7510424.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12507..13070)
                     /gene="ahpC"
                     /locus_tag="ACHKW5_000989"
     CDS             complement(12507..13070)
                     /gene="ahpC"
                     /locus_tag="ACHKW5_000989"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO7510425.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13442..14187
                     /gene="dsbG"
                     /locus_tag="ACHKW5_000990"
                     /pseudo
     CDS             13442..14187
                     /gene="dsbG"
                     /locus_tag="ACHKW5_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            14397..15299
                     /gene="ybdO"
                     /locus_tag="ACHKW5_000991"
     CDS             14397..15299
                     /gene="ybdO"
                     /locus_tag="ACHKW5_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7510426.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDAYVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15446..16666
                     /gene="ybdN"
                     /locus_tag="ACHKW5_000992"
     CDS             15446..16666
                     /gene="ybdN"
                     /locus_tag="ACHKW5_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7510427.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16639..17268
                     /gene="ybdM"
                     /locus_tag="ACHKW5_000993"
     CDS             16639..17268
                     /gene="ybdM"
                     /locus_tag="ACHKW5_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO7510428.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17269..18429)
                     /gene="ybdL"
                     /locus_tag="ACHKW5_000994"
     CDS             complement(17269..18429)
                     /gene="ybdL"
                     /locus_tag="ACHKW5_000994"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO7510429.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PHYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18538..19626
                     /gene="hcxA"
                     /locus_tag="ACHKW5_000995"
     CDS             18538..19626
                     /gene="hcxA"
                     /locus_tag="ACHKW5_000995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO7510430.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNYLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19636..19833)
                     /gene="ybdD"
                     /locus_tag="ACHKW5_000996"
     CDS             complement(19636..19833)
                     /gene="ybdD"
                     /locus_tag="ACHKW5_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7510431.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(20015..22120)
                     /gene="cstA"
                     /locus_tag="ACHKW5_000997"
     CDS             complement(20015..22120)
                     /gene="cstA"
                     /locus_tag="ACHKW5_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO7510432.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22301..22714)
                     /gene="entH"
                     /locus_tag="ACHKW5_000998"
     CDS             complement(22301..22714)
                     /gene="entH"
                     /locus_tag="ACHKW5_000998"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO7510433.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22717..23463)
                     /gene="entA"
                     /locus_tag="ACHKW5_000999"
     CDS             complement(22717..23463)
                     /gene="entA"
                     /locus_tag="ACHKW5_000999"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO7510434.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23463..24320)
                     /gene="entB"
                     /locus_tag="ACHKW5_001000"
     CDS             complement(23463..24320)
                     /gene="entB"
                     /locus_tag="ACHKW5_001000"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO7510435.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24334..25944)
                     /gene="entE"
                     /locus_tag="ACHKW5_001001"
     CDS             complement(24334..25944)
                     /gene="entE"
                     /locus_tag="ACHKW5_001001"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO7510436.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTLVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25954..27129)
                     /gene="entC"
                     /locus_tag="ACHKW5_001002"
     CDS             complement(25954..27129)
                     /gene="entC"
                     /locus_tag="ACHKW5_001002"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO7510437.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27504..28460
                     /gene="fepB"
                     /locus_tag="ACHKW5_001003"
     CDS             27504..28460
                     /gene="fepB"
                     /locus_tag="ACHKW5_001003"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7510438.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(28464..29714)
                     /gene="entS"
                     /locus_tag="ACHKW5_001004"
     CDS             complement(28464..29714)
                     /gene="entS"
                     /locus_tag="ACHKW5_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO7510439.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            29825..30829
                     /gene="fepD"
                     /locus_tag="ACHKW5_001005"
     CDS             29825..30829
                     /gene="fepD"
                     /locus_tag="ACHKW5_001005"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO7510440.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30826..31818
                     /gene="fepG"
                     /locus_tag="ACHKW5_001006"
     CDS             30826..31818
                     /gene="fepG"
                     /locus_tag="ACHKW5_001006"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO7510441.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31815..32630
                     /gene="fepC"
                     /locus_tag="ACHKW5_001007"
     CDS             31815..32630
                     /gene="fepC"
                     /locus_tag="ACHKW5_001007"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7510442.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32627..33760)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKW5_001008"
     CDS             complement(32627..33760)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKW5_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO7510443.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(33976..37857)
                     /gene="entF"
                     /locus_tag="ACHKW5_001009"
     CDS             complement(33976..37857)
                     /gene="entF"
                     /locus_tag="ACHKW5_001009"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO7510444.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADAHYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(37854..38072)
                     /gene="ybdZ"
                     /locus_tag="ACHKW5_001010"
     CDS             complement(37854..38072)
                     /gene="ybdZ"
                     /locus_tag="ACHKW5_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO7510445.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(38075..39277)
                     /gene="fes"
                     /locus_tag="ACHKW5_001011"
     CDS             complement(38075..39277)
                     /gene="fes"
                     /locus_tag="ACHKW5_001011"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO7510446.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            39520..41760
                     /gene="fepA"
                     /locus_tag="ACHKW5_001012"
     CDS             39520..41760
                     /gene="fepA"
                     /locus_tag="ACHKW5_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO7510447.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYVTTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSKNKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            41800..41898
                     /locus_tag="ACHKW5_001013"
                     /pseudo
     CDS             41800..41898
                     /locus_tag="ACHKW5_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41935..42555
                     /gene="entD"
                     /locus_tag="ACHKW5_001014"
     CDS             41935..42555
                     /gene="entD"
                     /locus_tag="ACHKW5_001014"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO7510448.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(42677..42829)
                     /gene="hokE"
                     /locus_tag="ACHKW5_001015"
     CDS             complement(42677..42829)
                     /gene="hokE"
                     /locus_tag="ACHKW5_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO7510449.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(43180..43332)
                     /gene="hokE"
                     /locus_tag="ACHKW5_001016"
     CDS             complement(43180..43332)
                     /gene="hokE"
                     /locus_tag="ACHKW5_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO7510450.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            43301..43570
                     /locus_tag="ACHKW5_001017"
     CDS             43301..43570
                     /locus_tag="ACHKW5_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510451.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            43774..44892
                     /gene="ybdK"
                     /locus_tag="ACHKW5_001018"
     CDS             43774..44892
                     /gene="ybdK"
                     /locus_tag="ACHKW5_001018"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO7510452.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            44958..45206
                     /gene="ybdJ"
                     /locus_tag="ACHKW5_001019"
     CDS             44958..45206
                     /gene="ybdJ"
                     /locus_tag="ACHKW5_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO7510453.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            45271..45639
                     /gene="ybdF"
                     /locus_tag="ACHKW5_001020"
     CDS             45271..45639
                     /gene="ybdF"
                     /locus_tag="ACHKW5_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7510454.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALIRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            45733..46386
                     /gene="nfsB"
                     /locus_tag="ACHKW5_001021"
     CDS             45733..46386
                     /gene="nfsB"
                     /locus_tag="ACHKW5_001021"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO7510455.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            46494..47741
                     /gene="ybdG"
                     /locus_tag="ACHKW5_001022"
     CDS             46494..47741
                     /gene="ybdG"
                     /locus_tag="ACHKW5_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO7510456.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(47809..49185)
                     /gene="pheP"
                     /locus_tag="ACHKW5_001023"
     CDS             complement(47809..49185)
                     /gene="pheP"
                     /locus_tag="ACHKW5_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO7510457.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(49287..52430)
                     /gene="cusA"
                     /locus_tag="ACHKW5_001024"
     CDS             complement(49287..52430)
                     /gene="cusA"
                     /locus_tag="ACHKW5_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO7510458.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(52442..53665)
                     /gene="cusB"
                     /locus_tag="ACHKW5_001025"
     CDS             complement(52442..53665)
                     /gene="cusB"
                     /locus_tag="ACHKW5_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO7510459.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(53681..54013)
                     /gene="cusF"
                     /locus_tag="ACHKW5_001026"
     CDS             complement(53681..54013)
                     /gene="cusF"
                     /locus_tag="ACHKW5_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO7510460.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(54037..55419)
                     /gene="cusC"
                     /locus_tag="ACHKW5_001027"
     CDS             complement(54037..55419)
                     /gene="cusC"
                     /locus_tag="ACHKW5_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO7510461.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            55576..56259
                     /gene="cusR"
                     /locus_tag="ACHKW5_001028"
     CDS             55576..56259
                     /gene="cusR"
                     /locus_tag="ACHKW5_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO7510462.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            56249..57697
                     /gene="cusS"
                     /locus_tag="ACHKW5_001029"
     CDS             56249..57697
                     /gene="cusS"
                     /locus_tag="ACHKW5_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO7510463.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        13894 a        15736 c        14634 g        13549 t
ORIGIN      
        1 atcaattaaa tcacttaaac ctgctggtca tttcgtaatt acattaatta aaaaacttaa
       61 aaccattaaa taaataaaaa accactaact ctatgtgaaa taaatcaaaa tttcacgccg
      121 aaatactcct taggatgtat agcgaaagga gaaaaagata tacctcgatc accccccttt
      181 ctcccaagtg aaaataaaag gttatcagtt tgcaacattg aacaacattc gttgcaaatc
      241 gataacaaca tgcaccttca ggatactatt tattatgttc ggcaatgata ttttcacccg
      301 cgtaaaacgt tcagaaaata aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga
      361 tttgagcgtt gacaccacag tcgaagtatt tattaccgta acccgcgatg aaaagcttat
      421 cgcgtgcggt ggaattgccg gaaatattat taaatgcgtt gctatcagtg aatccgttcg
      481 cggtgaagga ctggcgctga cattagccac tgaactgata aacctcgcct atgagcggca
      541 cagcacgcat ctgtttattt ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg
      601 tttttccacg ctgaccagcg tacccggcgt gatggtgctg atggaaaaca gcgccacgcg
      661 gctgaaacgc tatgccgaat cgctgaaaaa atttcgtcat ccagggaaca agattggctg
      721 cattgtgatg aacgccaatc ccttcacgaa tggtcaccgt tatctgattc aacaagctgc
      781 ggcacagtgc gactggttgc atctgttttt agtcaaagaa gattcttcac gcttccccta
      841 tgaagaccgg ttggatctgg tgttaaaagg caccgccgat attccacgcc tgactgtgca
      901 tcgcggctcc gaatacatca tctcccgcgc tacgttccct tgctacttca ttaaagaaca
      961 gagcgtcatt aaccattgtt acaccgaaat tgatctgaag attttccgtc agtacctcgc
     1021 tcccgcgctg ggtgtaactc accgctttgt cggtactgaa cccttttgtc gcgttaccgc
     1081 ccagtacaac caggatatgc gctactggct ggaaacgccg actatctccg caccgcccat
     1141 cgaactggtt gaaattgagc ggctgcgtta ccaggagatg ccgatatccg cttcccgggt
     1201 acgtcaactg ctggcgaaaa acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac
     1261 gctgcattat ttgcagaacc tgcttgagca ctcccgccag gacgcggcag ctcgtcaaaa
     1321 gacccccgca tgagaaacag gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc
     1381 cttgagtctg gggatgtgat gatacgcatc gccccactcg atacgcagga tatcgacctg
     1441 caaatcaata gcagcgttga gaaacagttt ggcgatgcaa ttcgcaccac cattctggac
     1501 gttctcgccc gctacaacgt gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg
     1561 gactgcattt tacgtgcacg actggaagcc ctgctggcac gcgccagcgg tatcccggct
     1621 ctgccatggg aggattgcca atgatttccg cttcgctgca acaacgtaaa actcgcaccc
     1681 gccgcagcat gttgtttgtg cctggtgcca atgccgcgat ggtcagcaac tccttcatct
     1741 acccggctga tgccctgatg tttgacctcg aagactccgt ggcattacgt gaaaaagaca
     1801 ccgcccgccg catggtttat cacgcgctgc aacatccgct gtatcgcgat attgaaacca
     1861 ttgtgcgtgt caacgcgctg gattccgaat ggggtgttaa cgacctggaa gccgtcgttc
     1921 gcggtggtgc ggacgttgtg cgtctgccga aaaccgatac cgctcaggat gttctggata
     1981 ttgaaaaaga gatcctgcgt atcgaaaaag cctgtggtcg tgaacccggc agcaccggcc
     2041 tgctggcggc gattgaatct ccgctgggca ttacccgcgc agtggaaatc gctcacgctt
     2101 ccgagcgttt gatcggtatc gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag
     2161 aacgctcccc ggaaggaact gaactgctgt tcgcacgctg ttccattttg caggccgcgc
     2221 gctctgcggg tattcaggcg ttcgataccg tctattccga cgctaacaac gaagccggat
     2281 tcctgcaaga agccgcccac atcaaacagc tgggctttga cggcaaatcg ctgatcaacc
     2341 cgcgtcagat tgatctgttg cacaacctct acgcaccaac ccagaaagaa gtggatcacg
     2401 cccgccgcgt cgtagaagcc gctgaagccg ccgctcgcga aggcctcggc gtggtttccc
     2461 tgaacggcaa gatggtggac ggtccggtta tcgatcgcgc ccgtctggtg ctctctcgtg
     2521 cagaactttc cggcatccgc gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa
     2581 tctcaacgac aagaacgggt agcggcctgg aatcgtcgcg ctgaatgcga tcttgccgct
     2641 ttccagaact cgccaaagca aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc
     2701 gccaacctgg aagaagcgat tcgtcgctct ggtttacagg acggcatgac ggtttccttc
     2761 catcacgctt tccgtggcgg tgacctgacc gtcaatatgg tgatggacgt catcgcgaag
     2821 atgggcttta aaaacctgac cctggcgtcc agctccctga gtgattgcca tgcgccgttg
     2881 gtagaacaca ttcgccaggg cgtggttacc cgcatttata cctccggcct gcgcggtcca
     2941 ctggcggaag aaatctcccg cggtctgctg gcggaaccgg tgcagatcca ctctcacggc
     3001 ggtcgtgtgc atctggtaca gagcggcgaa ctgaatatcg acgtggcttt cctcggcgtc
     3061 ccgtcctgtg atgaattcgg taatgccaac ggctacaccg gtaaagcctg ctgcggctcc
     3121 ctcggctatg caatggttga tgccgacaac gcaaaacagg ttgtgatgct taccgaagaa
     3181 ctgctgcctt atccgcataa tccggcaagc attgagcaag atcaggttga tttaatcgtc
     3241 aaagttgatc gcgttggcga tgctgcaaaa atcggcgctg gtgctacccg tatgaccact
     3301 aacccgcgcg aactgcttat tgcccgtagc gctgcggatg tgattgtcaa ctcaggctac
     3361 ttcaaagaag gtttctccat gcaaaccggc accggcggcg catcgctggc ggtaacccgt
     3421 ttcctggaag acaaaatgcg tagccgcgat attcgcgccg acttcgccct cggtggtatt
     3481 accgcgacga tggttgatct gcacgaaaaa ggtctgatcc gcaaactgct ggatgtacag
     3541 agctttgaca gccacgctgc gcaatcgctg gcccgtaacc ccaatcacat cgaaatcagc
     3601 gccaaccagt acgctaactg gggttcgaaa ggtgcatcgg ttgatcgtct cgacgtggtg
     3661 gtactgagcg cgctggaaat tgacacccag ttcaacgtta acgtgctgac cggctctgac
     3721 ggcgtactgc gtggtgcttc cggtggtcac tgcgataccg cgattgcctc tgcgctttcc
     3781 atcatcgtcg cgccgctggt acgcggtcgt attccgactc tggtggataa cgtgctgacc
     3841 tgcatcaccc cgggctccag tgtcgatatt ctggtcacag accacggtat cgcagttaac
     3901 ccggcacgtc cggagctggc agaacgtctg caggaagcgg gcattaaagt ggtttccatt
     3961 gagtggctgc gtgaacgtgc gcgtctgctg accggtgaac cacagccgat tgaattcacc
     4021 gaccgcgtcg ttgccgttgt gcgttaccgc gatggttcgg tgatcgatgt tgtacatcag
     4081 gtgaaggaat aagccatgca cctgcttcct gaactcgcca gccaccatgc ggtgtcaatt
     4141 cccgagctgc tcgtcagccg ggatgaaagg caagcacggc aacacgtctg gctcaagcgc
     4201 catcctgttc cactggtctc ctttaccgtg gttgcgcctg ggccgattaa agacagtgag
     4261 gtcacacgcc gaatttttaa tcatggcgtg acagccttgc gtgccttagc cgcaaaacag
     4321 ggctggcaaa ttcaggagca ggctgcactg gtttccgcca gcgggccgga gggtatgttg
     4381 agcattgccg ccccggctcg cgacctcaag ctcgccacca ttgagcttga acatagtcat
     4441 cctctcgggc ggttatggga tatcgatgtc ctgacgcccg aaggcgaaat tctctcccgc
     4501 cgcgactatt cactgccgcc tcgcagctgc ctgttgtgcg agcaaagcgc agccgtctgc
     4561 gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac
     4621 gatgtcgatg cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga
     4681 gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg
     4741 cctcgtggat cggattaact gcggtgcgca taaagatatg gcgctggaag atttccaccg
     4801 cagcgcgctg gcgattcagg gctggctacc ccgtttcatt gaatttggtg cctgtagtgc
     4861 ggaaatggca ccagaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg
     4921 tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg
     4981 gctgctatgt gcggcaattg gccgtttgct tcagctcaac cagccggtaa cgccaacaac
     5041 cgtttgttct acggcggcaa gtttctgccg tggtctgacc gatcgcgaac tgcgtaccaa
     5101 taattcacaa ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg
     5161 cggtgaagcc gaagcgggtt atccactggt gatcaaccac gccttgccgc attacctcac
     5221 tctgctggat caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat
     5281 ggcgatcaac ggcgatacca acgttgcttc gcgcggtggc gaggggggcc tgcgctggct
     5341 acagcgcgag gcgcaaacat tattgcaaaa agggggcatt cgaacccccg ccgatctcga
     5401 ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc
     5461 cgacctgctg atccttacct ggtttttagc acagatttaa ttatttaagc acttgataaa
     5521 tttgaaaata ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa
     5581 ctattggccc cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg
     5641 ccaccacagg catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc
     5701 gagccaattc cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt
     5761 aattacctgc tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag
     5821 gcgctgaaat ggggtctggc tggtttttcc agcaccacgg tatggctggt atttggcgca
     5881 tttatttttg cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg
     5941 gtgaaattca tgggcaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt
     6001 ctgctggcac cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc
     6061 attaaaaacc tgccgccgtt gtttaaatca ttccccaacg atccgtccgc gcgtcgtatt
     6121 ggcggctatt tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc
     6181 accggtgcgg caccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag
     6241 attagctggt tgcagtggtt cctctgcttc ctgccggttg ggggtatctt gcttatcatt
     6301 gcgccgtggc tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca
     6361 acctgggcgg gtgatgaact gaaaaccatg ggtgcgctga cacgcagaga gtggacactg
     6421 attggccttg tattgctcag cttaggtttg tgggtttttg gcagtgaagt cattaatgct
     6481 actgcagttg gtctgctggc agtttcgcta atgctggccc tgcacgttgt gccttggaaa
     6541 gatattaccc gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg
     6601 atggctaacg gcctgactcg ttctggcttt attgactggt tcgccggtac catgagtacg
     6661 cacctggaag gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt
     6721 gcacactacc tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg
     6781 gccgtcggta aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg
     6841 tctatcggta tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac
     6901 ggctgtggct atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt
     6961 tacatctcta tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt
     7021 cgccaaacgc tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta
     7081 tgatttaata gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct
     7141 ggcgtaacgc cgcgttgctc gcggtttctc tgcttccctt ctcttctgcc aacgccttag
     7201 cgttgcaggc aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa
     7261 ccggattttg ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc
     7321 aaaccgaaac gaccaacaaa gctgattttc tgaccgtaca tggtctgtgg ccaggattgc
     7381 ctaaatcggt tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta
     7441 ctcgcccaat cccgaatcta ccagaagcgc acgccagccg gatgtgttca tcgccggaaa
     7501 ccggattatc actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac
     7561 gttcctgcct ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg
     7621 acgcatactt cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa
     7681 aattccttgc ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg
     7741 ccaaaagctg gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg
     7801 cgtatttgac agaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg
     7861 caaactcatt cttgccacaa ccacacccag gcaactgtgg caaaaccttt gtgattgata
     7921 aagtgggtta ttaatcatct gatttacaaa acacgctaat tcgtcttcca taaaccgtga
     7981 cgattaagaa ttatacataa tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca
     8041 cttcaaagag tgagcaacag tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg
     8101 tgagggtttt cttttgggta tgtttatctg cgacactcgc agtaccgacg acatggagta
     8161 aaaatgtcca gaccaactat catcattaac gacctggatg ccgaacgcat cgatattctg
     8221 ctggagcaac ccgcctatgc tggtttgcga atcgccgacg cgttaaacgc agagttggat
     8281 cgcgcccaaa tgtgttcgcc agaagagatg ccacacgacg tggtgacaat gaacagccgg
     8341 gttaaattcc gcaatcttag cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca
     8401 aaaatgaccg atagcaatac tcagctttcc gttatggctc cggtaggtgc cgcactgctg
     8461 gggctgcgcg ttggcgattc cattcactgg gaacttccgg gcggcgttgc aacccacctt
     8521 gaagtgctgg aactcgaata ccagccagaa gctgctggcg actacctgct ttaatcccat
     8581 ctgcacaagc gataactcgc tttgcccaga ggatgcactc gcatcctctt tacgcaaaat
     8641 cagcaatttt tcctggttct cactgttcac ttttgctgca aaattattcc gtttcgtttg
     8701 tgggaataac catggcagaa gcattttaca tcctgatagg atttctgatt atggcggcga
     8761 ttatcgtcat ggctgtcctt tacctcgaaa accactcctg acgatcatat tgttcccccc
     8821 ggcatcgcat tcaccagacc tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg
     8881 ggtaccagaa tcaccttacg gcactcctct tcacgttttt cgaaaatctc atatccccgg
     8941 gcggcctctt aaaacggcat atagtgggta acaatttctt ctggtttcag taatcctttc
     9001 tcaattaacg gtaataattc tcccagccat gcgtgaacgt gggtctgtcc cattttaaac
     9061 gtcaaccctt tatcaaaggc gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg
     9121 cccggtacgc taacaatgcc gccacgcctg accgccgcaa tacactggcg caacgcttta
     9181 ccgctgctgc cctccagttt caggttagtc agcaccgttt ccgtggtgct gcctttcgct
     9241 tcaaaaccga cggcgtctat tactgcatcc acgccccggt gacctgccgt ttgttcaata
     9301 attgactgtg ccggatcgct atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca
     9361 gcggcgaaac gcaagcggta gggatgatga tcaacaacaa aaatctgttc cacaccgagc
     9421 aaccgtgcac aggcgattgt caacaatccc actggaccgg caccatagac tgcaacgctt
     9481 gaaccttgtt ggatctgcgc attttttgct gcctgccatg ccgttggcag aatatcagaa
     9541 aggaaaaggg ctttatcatc tgaaagcaaa ggcggtactt taaacggccc cacattccct
     9601 ttagggacgc ggacatattc cgcctgtcca ccaggaacgc caccatacag gtgactataa
     9661 ccaaacaatg ccgcgggagc tggtatctgt tttttattga gcgcagcgcc tttacccgca
     9721 ttggtatttt cgcaggcggc atattgctgc attcgacaga aaaaacagtc gccacaagca
     9781 atgacaaacg gaattaccac ccggtcgcct ttttgcaaat tttttacgtc ctttccggtt
     9841 tcaacgactt cccccataaa ttcatgacca aaaatatcgc catgtttaac ctggggtatt
     9901 ttgcctcgat aaagatggag gtcagagcca cagatcgccg ttgccgtaat acgcagaata
     9961 atatcatctg cctgttcaat gcccggatcg ggaacatttt ctacctgaac gtgatgtggg
    10021 ccgtgataag tcaatgcttt catgcaacct ccagagacag ttcaaaaaag agtcagtctt
    10081 taagggtagc tatcacagcg ggaaaacggg cggtaccgca ggttttggga gaattcttgg
    10141 tgttcagtga gaatatatta atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat
    10201 atcagggata aatattataa tcatggcgaa tgatttgttc atgattaaca gggagaaagg
    10261 gttatgtata agacaatcat tatgccagtt gatgtatttg aaatggaatt gagcgacaaa
    10321 gctgttcgcc acgctgaatt cctcgcccag gatgacggag ttattcatct acttcacgta
    10381 ctacccgggt cagccagcct gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa
    10441 gagcatctgc aacatgaagc agaagaacgt ctgcaaacga tggtcagcca cttcaccatc
    10501 gatccttccc gcattaaaca acatgtccgt tttggtagcg tgcgggatga agtcaatgag
    10561 ttggcagaag aactgggggc tgatgttgta gttattggtt ctcgcaaccc atcgatttcg
    10621 acccatctgt taggttctaa cgcctcgagc gtgatccgcc atgccaatct gccggtgctg
    10681 gttgtgcgtt aattgccaga ataaatatcc cgccctgccc ggagccatcc cggcagggct
    10741 ttttaatgcc ataaaaaagc cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa
    10801 tcttacttct tcttatgcag ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc
    10861 tttggcacct tcgccagtgg cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc
    10921 cgctgcgaat acgcctttga cgttggtttc gcatttcgca tcaatgataa tctcacccat
    10981 gcggttacgt tcgactgcgc cttccagcca gttggtgttc ggcagcagac caatctggac
    11041 gaaaataccg gccagttcga tgttgtgaat atcgccgctg acacgatctc gatattccag
    11101 accaacgact ttgctgccgt cgcctttcac ttccgtggtt tgcgcattca gaataatgtc
    11161 gacgtttttc aggctgcgca gtttgtcctg cagcacctgg tcggctttca tttctggcgc
    11221 aaattccagc agagttacgt gctcaacgat acccgccagg tcgattgccg cttccacgcc
    11281 ggagttaccg ccgccgataa ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg
    11341 cgggcagtag gtcacgcctt tggtgcgata ctgatcttcg cccggaacgt tcatgttgcg
    11401 ccattttgca ccggtcgcca cgataatgct gcgtgctttc agtaccgcgc cagaagctgt
    11461 ttcaatctgg tgtagaccac cttcaaccgc tgccgggatc agtttgctgg cgctctggct
    11521 gtcgatcaca tcaacgtcgt attcatcaac gtgaactttc agtgcgcctg ccagcttctg
    11581 cccttcagtc ttcggtacag aaatgtagtt ttcgatatca acggtatcga ggatctgacc
    11641 accaaaacgt tcgcccatca gaccggtacg gatgccttta cgtgcggagt aaattgctgc
    11701 cgctgcaccc gccgggccgg aaccgacgat taatacgtca taagcatcac gcttgttcag
    11761 ctcttctgcc gcacgttttt ccgcgccagt atcaattttg gcaacgattt cagtcaacgt
    11821 catgcggccc tgaccaaact ctttcccgtt tacgaacact gccggaacgc ccatcacgtt
    11881 gcgatcggtg atttcgttct ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg
    11941 gttcagtacg ctcatcaggt tcagcgcctg caccacgtcc gggcagttgt ggcaagagag
    12001 cgagtaatag gtttcgaatt caaaatcacc gtcaatatgg cgaatctgct ccagcagaga
    12061 ctgcgcttct ttcgacggat gaccaccggt ccacagcaac gccagtacca gcgaggtgaa
    12121 ctcgtggccc agcggggagc ctgcaaaacg tggcccctgg ttggaacctg ggttggtgat
    12181 caggaaagac ggcttacgca ccggcaagct gttatcttct ttaaaggtga ctttgtctga
    12241 cagttctgcg atttcagcca acagttcctt gatttctgcc gatttagcgc tgtcatccag
    12301 cgtggcaatt aactcaacag gcttggtcaa tttctcaagg taagccttga gttgagtttt
    12361 catatttgtg tcgagcatgt ttatctcctg ggctttaaac atcatcatgc aagcggcctc
    12421 tgcggctacc tggatgcagc ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca
    12481 cccgattaat tacgcgattg cggaacttag attttaccaa ccaggtccag agacggagcc
    12541 agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg agaagctacg
    12601 tactgtgctg ctttgatttt acgcagcagg tcagacgcgt cacggccaat gccttcagcg
    12661 gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc
    12721 agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg
    12781 atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg
    12841 aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag ttcttcgtag
    12901 tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc cgggtagaag
    12961 aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt
    13021 ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat ctatacttcc
    13081 tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta atgcgtttcc
    13141 attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt gaactttgca
    13201 ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt aatctgaact
    13261 accctttggt atcttcagcg cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca
    13321 acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta agctcttacc
    13381 tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa aggacaaatt
    13441 aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg cagaggaact
    13501 tccttcccca gtaaaagcga ttgaaaacag ggcattacaa tcatcaaaac gttcgatgcc
    13561 cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac catctacctg
    13621 actcccgatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg tgaaaacctg
    13681 agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat gtggcaacgg
    13741 atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat tgtctacgtg
    13801 ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg cccgtgggta
    13861 gattctggca aagtgcaatt aagaacattg ttggttgggg ttatcaagcc agaaagcccg
    13921 gcgacagcag cggcaattct tgcctccaaa gatcccgcaa aaacctggca agaatatgaa
    13981 tcctctggtg gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga acaaatgaaa
    14041 gtgttaagcg ccaacgagaa actcatggac gatctggggg caaatgtcac accagcgatc
    14101 tattacatga gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga tcagaaaacg
    14161 cttaatatca ttatggggaa taaataaaag ataattcatg gatggttgaa atacatccct
    14221 gcttttcaac catcctaaaa aagcatgaat tgctcgctct gccctaagtg gctatgctat
    14281 tttagaaaaa cttaatcctg agttaagcaa aaaaaatata atcaataaaa atatatacgc
    14341 aaaaaacatt gattaagtga atataccatg gaagaaaaat ataaccggag tagtgtatgg
    14401 ccaatctcta cgacttgaaa aagttcgact taaaccttct tgtcatattt gagtgtattt
    14461 accagcattt aagcattagc aaagctgctg aatcactata tatcacccca tcagccgtga
    14521 gccagtcgtt acaacgctta cgtgcgcaat ttaatgaccc tttattcatt cgctcaggaa
    14581 aaggtatcgc gccgacgaca acagggttga atctgcatca ccatcttgag aaaaacctca
    14641 aaggccttga acaaacaatt aatatagtca ataagtccga actaaagaaa aactttatta
    14701 tttacggccc tcagctcatt tcttgctcta ataatagtat gctgatccgc tgcctacggc
    14761 aagatgccta tgtcgagatt gagtgccatg atatcctcat gtcagcagag aatgctgaag
    14821 aactgttggt tcaccgcaaa gccgatctgg tgatcaccca aatgccagtg atcagccgtt
    14881 ccgtcatttg catgccattg catacaattc gcaatactct aatttgtagt aataagcatc
    14941 cgcgaattac tgacaatagc acttatgaac aaattatggc agaagagttc acccaattaa
    15001 tatccaaatc agcaggagtt gatgatattc aaatggaaat agacgagaag tttatgaatc
    15061 gcaaaatttc atttcgtggt tcctctttgc tgacaatcat taatagcatt gcagttacag
    15121 atttactggg aatagtcccc tatgaacttt ataattccta ccgtgacttt cttaatttaa
    15181 aagagataaa actagaacat ccgttacctt caataaagct ttacatttcc tataataaat
    15241 cttctttaaa taatttggtg ttctcccgat ttattgaccg tctaaatgag agtttttgat
    15301 atataactac agacagctac tataactttc atctatttat tcacagcgcg aatgaaaaat
    15361 aaaacactct gctttgcagt gtttacttta taattaacag acttatctca ctgatcaccc
    15421 tgtaacgttc agagagcgtc ttcagatgtc tatttataaa attcctcttc cgctcaatat
    15481 tctggaagct gcacgagagc gtattacctg gacactcaac accctacccc gcgtttgtgt
    15541 ctcattttct ggcggcaaag actctggttt aatgctgcat ctgacagccg aacttgcccg
    15601 acaaatgggc aaaaaaatct gcgttttgtt tatcgactgg gaggcgcaat tctcttgcac
    15661 cattaactat gttcagtccc tgcgcgagtt gtacaccgat gtcatcgaag agttttactg
    15721 ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa ttccaacccg aatggcagtg
    15781 ctgggaacct gatgtcgaat gggtgcgtca gcccccgcaa gatgcgataa ccgatcctga
    15841 ctttttctgc ttttaccagc ctggcatgac cttcgaacaa tttgtacgtg agtttgccga
    15901 atggttttca caaaaacgtc cggcggcgat gatgatcggc atccgtgcgg atgagtccta
    15961 caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt tttgccgacg ataaaccatg
    16021 gactacggcc gcaccaggcg gtcatagctg gtacatttac cccatttacg actggaaagt
    16081 ggctgatatc tggacctggt atgcaaatca tcaaagtctc tgcaacccac tgtataacct
    16141 aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga
    16201 gcaacgacaa ggattatggc tctatcacgt tatcgaaccg gatcgctggg cagctatgtg
    16261 cgcacgagtc agcggggtaa aaagtggcgg catttacgcc ggacatgaca atcatttcta
    16321 tgggcatcgg aaaatcctca agccagaaca tttagactgg caagaatatg cattattgct
    16381 gctcaatagc atgccggaaa aaacagctga gcattaccgc aataaaatcg ccatttattt
    16441 gcactggtat cagaaaaaag gcatcgaggt tccacaaacc cagcaagggg acattggcgc
    16501 gaaagatatc ccctcctggc ggcggatatg caaagtttta ctcaataacg attactggtg
    16561 tcgagcatta tcatttagtc ctacgaaagc gaagaactat cagcgttata acgaacggat
    16621 aaaaggaaaa cgtcaggaat gggggatact atgcaacaac gattaacgca ggatttaacg
    16681 caatttctcg ccagcttgcc ggaagatgat cgcatcaaag cgattaatga aatccgcatg
    16741 gcgatccatc aggttagccc ctttcgtgaa gagccagtgg attgcgttct gtgggttaaa
    16801 aacagccaac ttatgcctaa tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa
    16861 ctgctgaaaa aatccattga aatagatggt tttacccaac ccattgtggt cacgcatacg
    16921 gataaaaatg ccctggaaat cgtggatggt tttcaccgcc atgaaattgg taaaggctca
    16981 tcatcattaa aattacgctt aaaaggttat ttgccggtga cctgtctgga aggtacgcgt
    17041 aatcaacgca tcgccgcgac tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc
    17101 gccatgtcgg aaatcgtccg tgaacttagc cagttgggat gggacgataa taaaattggc
    17161 aaagagctgg gaatggacag cgacgaagtg ctgcgcctga agcaaattaa cggcctgcaa
    17221 gagttgtttg ccgaccgtca atattcccgc gcctggacgg ttaaatagct aaagctggcg
    17281 caggcgttca gctgctgcca gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag
    17341 tttatgtggg aagggatcgg cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc
    17401 ctgcgtcagc cactggcaaa actcaacatc atccagggta gaaaccgcgc tgtaatccac
    17461 cagcaaaaag tatgtacctt cacacggtaa aatctccagc cggctttcat ttaaagcatt
    17521 caccagaata tcgcgcttct ggcgataaaa gtccggtaac gcaagataat gctcaggttc
    17581 tgcacgtagc atatcagcaa gcgccagctg tgccggggta ttcaccgaaa aggtcagata
    17641 ctgatgtacc ttgcgaattt cggcgctgat gggcgctggc gcaacacaat aacccacttt
    17701 ccagccggtc atatgatagg tcttgccaaa tgaagaaacc gccactgccc gctcacgcag
    17761 ctgcggatgc gccagcacac tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac
    17821 ttcatcgcta atgacaaaaa tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc
    17881 agcctgctgc cagatagttg cactggggtt atgcggagtg ttgaggatca ccagtctggt
    17941 gcgctcgctt aataatgcgg caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag
    18001 tgccatacgc ttcactattc ccccagaaag cgcgatggcg ggggagtaac tgtcatagct
    18061 gggatcaaaa caaatcactt catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa
    18121 cgcttccgtc gcccctgccg ttacggtgat atcgctatcg gcatctggtt gatagccata
    18181 caaacgttcc gttttctgag caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc
    18241 gtattggttt gccccctggg caacgtggta cgccagccgc tcctgtaaat agtgcggacc
    18301 atcaaaatca ggaaagcctt gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct
    18361 catctgggtg aaaatagtgg tgccaagttg tggaagtttg ctttgtggaa tcagagggtt
    18421 atttgtcatt tttattgtac ctgacgaagt tgcgtgttga aggcactata acacgatgct
    18481 agtatttggc aatcaagacg tttagatgtc taaataaagc aataaggaca acacaacatg
    18541 cctcacaatc ctatccgcgt ggtcgtcggc ccggctaact acttttcaca tccaggaagt
    18601 ttcaattacc tgaacgattt tttcactgat gaacaacttt ctcgcgcggt gtggatctac
    18661 ggcaaacgcg ccattgctgc ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg
    18721 gcaaagcata ttttgtttcg tggtcattgc agcgaaagcg atgtacagca actggcggct
    18781 gagtccggtg acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc
    18841 gcgaaagccc tcgcccgccg tctcggtcta ccatttgttg ccgctccgac gatcgccgcc
    18901 acctgcgcag cctggacacc gctctccgtc tggtataacg atgccggaca ggcgctgcat
    18961 tatgagattt tcgacgacgc caattttatg gtgctggtgg aaccggagat tatcctcaat
    19021 gcaccgcaac aatatctgct ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg
    19081 gtggtgctgg ctccgcaacc agaaacgttg ccgctaaccg tgcggctggg gatcaataat
    19141 gcgcaggcca ttcgcgacgt cttgttaaac agtagcgaac aggcacttgc cgatcagcaa
    19201 aatcagcagt taacgcaatc attttgcgat gtggtggatg cgattatcgc cgggggtgga
    19261 atggtcggtg gtctgggcga tcgttttacg cgtgtggctg cggcccatgc cgtgcataac
    19321 ggtctgactg tcctgccgca aaccgagaaa tttctccacg gcaccaaagt cgcttacgga
    19381 attctggtgc aaagcgcctt gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg
    19441 tatcagcgtt ttcatctgcc gactacgctg gcggagctgg aagtggatat caataatcag
    19501 gcggagatcg acaaagtgat tgcccacacc ttgcgcccgg tggagtccat tcattacctg
    19561 ccagtcacgc tgacaccaga tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa
    19621 gcctgacgtg caaatttagc agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg
    19681 ccgctcacgg aaaaactcct cgtaggtcat cggcgtttga tcgggatggt taacccgcat
    19741 atgttcgaca tagttgtcgt aatcaggcat accaatcatc agcttcgccg cctgacctaa
    19801 atattttccg gcttttgcca gtgaatcaaa catcttggcc tccgctaacc atgttgctga
    19861 aagagtgccg gatgcggcgt gaacgcttta tccggcctac aaatgctatc aacgcactcg
    19921 gtcagtcccc tcgccccatc ggggagaggg ttagggtgag ggggaaccct cggcactatg
    19981 ccaacattcg ccaacatccc ccctcactct gactttagtg cgcacctttc gcctgcgcta
    20041 cgatctcctc aacattttcc ggcattggct catacggcgt ttctttcgcc gttggcttcg
    20101 gctctttcaa tgccgcaagt gccgtcttaa tcgagaacag tgccagaacc accacgacca
    20161 ccataaagaa gatggttaac ccggcatcca gacggttgtt gaacaccagt tgcgccagct
    20221 gtgactcagt atactgcgac ggaatattgc cgctgtcgat cattgcctgg aacttgttag
    20281 caatggccag gaagccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca
    20341 gggtacaaat cagtagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt
    20401 tcatcttgaa caacaccacg gcacagagca tcagcgccat ccctgccagc atctggttgg
    20461 caataccaaa cagcggccac agagtgttaa taccgcccag cggatcgacc acgccctgat
    20521 ggaggaagta gccccacgcc agtacgcaca gcgccgttgc cagcaggtta gcaggcagtg
    20581 aatcggtccg cttcaggcca ggagacacca cgcccagcag atcctgcaac ataaagcgcg
    20641 cagcacgcgt acccgcatct accgccgtca gaataaacag agcttcaaac agaatggcga
    20701 agtgatacca gaacgccaca tccatcatac cacccagcgc accgtgcagg atgtaagcca
    20761 tccccaccgc caacgtcggc gcaccgcccg cacgggaaat gatcgactgt tcacccactt
    20821 cgctggcaat ctggttgagc gtatctggtg taatgctaaa gccccagcta ctcaccacct
    20881 gcgcggcaga agcgaccaca tccgccgtcc ctgccggagc cagcaccgcc attgggctgt
    20941 tcatggcaaa gtaaacgccc ggatcgatga tacaggcgga aaccagcgcc ataatcgcca
    21001 cgaaggattc catcaacatt ccaccgtagc cgataaaaca agcctgccct tcgttcgcca
    21061 gcatcttcgg cgtggtgccg gaagagatca gcgcatggaa gccagacacc gcgccacagg
    21121 cgatggtgat aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccatcga
    21181 caaatttggt cagcgcaggc atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca
    21241 gaccgacgat cgtcccgatt ttcaggaagg tagagaggta gtcacgcggg gccagcagca
    21301 accacaccgg cagcaccgcc gccacaaaac cgtaacccac cagcatccag gtcagctgca
    21361 cgccggtaaa gtcaaagtac ggtgcccagg tcggactttc tgccacccag ccgccagaga
    21421 taatggcgaa aatcaggaat accagaccga tgaccgacac ttcaccaata cgccccggac
    21481 gcagatagcg caggtagatc cccataaaca gcgccagcgg aatggtgaac gcaacggtat
    21541 acgttcccca cgggctatga gtcagggctt tcaccacgat catcgccagt actgcaagga
    21601 taatgaccat gatcataaag caggccacca gcgctatcac cccggcggtt ggccccatct
    21661 cttctttgac cagctcaccc agcgatcgac cgtcacggcg cgtagaaaca aacagcacca
    21721 tgaaatcctg caccgcaccg gcgagaacca ccccggcgag cagccagatc atccctggca
    21781 ggtaacccat ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg
    21841 caaaatggtg accgaacagc actttcttgt ccgtcggcac atagtccagc ccgtcgttat
    21901 ggcgcaccgc tggcgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca
    21961 gcccataaaa acggtaagcg atcagataga tacagaccga cgccaccaca atccacagcg
    22021 cgttgatctg ttccccacga tttaaagcaa tgtatcccag agcaaatgct cccattacag
    22081 agagcactgt ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg
    22141 tccatagata tgttacattt tgtttctata acatctttgt acgatttaac aacacggtaa
    22201 ccatgtaaat cggagttgaa accaatattt aacttaacaa tgttaactcg atcactccgt
    22261 taaccgagtt ttcctgcaat ctcatttaat tctgtcgggc tcatcccaaa actgccgtac
    22321 ccagccgaca agtgcagcaa cgccgcccct gttcatcgaa aacgacgatt tcccagctct
    22381 gattttgccg cccaagatgc agcggctggc agacgccgcg taccttgcct tcagacaccg
    22441 ggcgatgatg ggtggcgtta agttccgtac ccaccacaca ctgcccgtca cgggtcatca
    22501 taaatccggc catcgatccc agcgtttcag ccagcgccgc tgacgcgccg ccatgcagca
    22561 ggccaaacgg ctgatgagta cgggtatcaa ccggcatttc ggcttccagc acatcatctc
    22621 ccagacgggt atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca
    22681 gttcgtcgag cgttaaatgg cgtttccaga tcatgcttat gcccccagcg ttgagccgcc
    22741 atcgaccaca atatcctgta gggtgatatg gctggcgagg tcagaggcga ggaacaaaat
    22801 cgtgttggcg atctcttgtg gacgggcgat tttccccagc ggaatgccga gtttaaactg
    22861 ctcgccaaag ccgcgaatac gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg
    22921 catatcggtg tcggtggagc caggcgaaac cacattacag cgcacgccgc tacccgccag
    22981 ttccagcccg acgctcaacg ccaggctttt cagcgccgct ttcgatgcgc cataagcact
    23041 catgccaata cgcggcgtgt gcgcggcgtc ggacgccaca gtgacaatcg ccccgccccg
    23101 ctgacggcga aactggttca tggtttgctg gaacaggtta aacgcaccgc cgacgttaac
    23161 cgcaaaagtc tgctgccagt cctctttact gagctgatcg gtcgcgccca tgcgcaaaat
    23221 tcccgccgca ttgaccagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac
    23281 ctgcgcgacc tgcgcagcat cggcaacatc catcacttcg gtcgcaaagg gatattgctc
    23341 ctgagcgaac gcttgatcaa aaccggtaac tttcgctccc gcctcaacaa acgccagcgc
    23401 cgtggcgtag ccgatacctt tacctgcgcc ggttacccag acatttttac cgctgaaatc
    23461 cattatttca cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc
    23521 agcatgacaa agtcgatatc accatgcact ttgcgccagc gcgccgccag cgccatcatg
    23581 cgcaccgaat ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg
    23641 tccagcaacg gcaggataac ctcacgcagc gccgctttgc tggcggggac aggtgctggc
    23701 agtaattctt cagtcatcac cacccggcca gaccgcccgg ccacatattt cagcgacatc
    23761 aaatgctcgt cacggctgaa atcggccagc gcatccgcca ccataaacgg tttaatatcg
    23821 cgcataaatg cgtcggttgc ggtggtcata cagccaatgt gggcatatac cccggtaata
    23881 atcagctggt tacgtccact ctctttgagc atttgctcca gcggagaacg atgaaacgcg
    23941 ctgtagcgcc acttcaccag caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc
    24001 ttttgctgtt ccggcgaacg ggtcaggccc ggcccccaca tatcattcaa cagcgcccga
    24061 tcttcatcgc tctgctcttt cggctgggcg gtgtaataaa ccggaatatt gtgctgtttg
    24121 cagtagtcgc gcagcgcagc aatattcgcg atcacctgct ccatcatcgg gcagttctcg
    24181 ccccagaagc tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt
    24241 tcaaaggccc agtcaacttt attcttaggg atatcgtgag actccggcag tgcgtaagcc
    24301 tgtaattttg gaatagccat cgtgttctct ccttcaggct gatgcgcgtg acgccagcca
    24361 ctgacgtaat tgttttttat cgactttccc gaccgccgtc agcggaagtg aatctacaca
    24421 ctccacgcga tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac
    24481 ctgcaccgcg cgcagcggct cttttaccac cagataagcg cagctttttt cgcccatcag
    24541 ctcatcttcc atgctcacca gtgcggcgta gatcaccgcc gggtggcgca gcagcaggtt
    24601 ttcgatctct tcggcagcga tcttctcacc gccacggttg atctgatctt tctcgcgccc
    24661 ctgcacggtg atgtacccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc
    24721 gttggcatca aaggcactgg cattgtgctg cggacttttg taataaccgc ggaaggtgta
    24781 cggcccgcgc gtcatcaggc gtccaacttc cccttgcggc agtggatttc cttcagcatc
    24841 agcaacccat acttcatcgt ccggacacat tgggtaaccc tgggtatgga taattttctc
    24901 cgcgctatca tcaagtcggg tgtagttcac cagcccttcc gccatgccaa acacctgctg
    24961 caacaggcag ccaatctcag cgggaatacg cgccgcaagg gtggctgaaa gacgtgcgcc
    25021 gccgacctgt aacagtttca gcgaggcaag ctgcgcccgg ctttcacctt cggtcagcgc
    25081 ctgcaaccac aggctgactg ccggtggcac cagcgcggtg acgttcacct gatgtttttc
    25141 aatcaatggg aagcaaagcg tggcgctggg atcggccgcc agaacaagcg ttcctccggc
    25201 aagaaagacg cccagcgatc ccggcgaact catggcgtag ttatgagccg ccgggatcgc
    25261 gcacaggtag cgtgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta
    25321 gtagtagtcg ttatgagtgc gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag
    25381 ctggaaatag gccacttcat cagcaggtga tggcgtggca gtaaaatcct cagccggatg
    25441 gttaatcgca tcctgcaagt tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat
    25501 ggaggaatgt tctgtgacga aagtattgag gaaatcatcc ccgctaaaca gcgcatgttg
    25561 gcgatcggca atcagcaatg cgggttcaat ctggctggca taggcgttca gttcactacg
    25621 ctgatggctg aacagcgcca gcaccggcgc aacgcccagt ttcagcagcg cgaaaaaggt
    25681 aatatacaat tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc
    25741 ctgacggcgt aaactacacg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa
    25801 ctgtcgctcg ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc
    25861 ggtcagcggc aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg
    25921 ccagcgggtg aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat
    25981 ggtagaaagt ttgacgcctg tttcgcgcca ctcacccaac ggtgacgacg caggcacaat
    26041 ccccgctccg gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac
    26101 cacccattcg ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg
    26161 gtcgaatggc tccagttcag caataacctg ggtcgcggcc tgatgcggga aaccgctcag
    26221 cgcgggggtt ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt
    26281 accttcaaag ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga
    26341 gggaacgtgt aactcactac tgcgttcgcg cagtacctct ttcatcgcct gagtcaccag
    26401 ttcatgttca tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag
    26461 cacttcatcc ggctgacgac gcgcggaacc ggctaacgga atggagctaa aacgctcgcc
    26521 gtctttacgt agcagcagtt ccgggctggc ccccagcagg acgccaccat cagccagcgg
    26581 gacatggaag ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact
    26641 atcaatggcg gcgtcagtgg tgatatcaat caatcgtgac aacaccactt tgtcgacctg
    26701 cggcgtggcg gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg tttgttcagg
    26761 aattgcctgg cgttccacca cgttcagcga ctggctgcgg gtaaaacggc gggctgaggt
    26821 ctgtttttcc tggcgggaga acgactgcca ggattcggga atatacagcg acgaaggctg
    26881 acgtggatcg aagggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc
    26941 atcggcaaac agcgcggcga gtttttgctg gaagggactg tcgggcgaat ccccgttcac
    27001 agccggttca tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat
    27061 aaagaaaaag cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga
    27121 cgtatccata tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt
    27181 ttcggtcgct aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg
    27241 caactcgggg ttgcatctgg ttgtggttac aatgaaaatg agaagcatta ttgatggatt
    27301 cgcataagcg caatgtgatg tcctgcgccg ttctgccccc tctcccttcc agggtgaggg
    27361 ctggggtgag ggttaatgtt cgcaccagtg ctggctgttc ccctcaccct aaccctctcc
    27421 ccaaaggggc gaggggacgg attgtgcgct ttgtcgaatt tgtcattacg cccttaacct
    27481 tattaataac aggaagctga tttgtgagac tcgccccgct ctaccgcaac gcccttctat
    27541 taacaggact tttgctttca ggaatagccg cagttcaggc cgctgactgg ccgcgtcaga
    27601 ttactgacag ccgtggcaca catacactgg aaagccagcc gcagcgtatt gtttccacca
    27661 gcgtcaccct gaccggctca ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga
    27721 ccacgccgaa taaccgcgtc gcggatgacc agggcttttt acgccagtgg agcaaggtgg
    27781 cgaaagaacg caaactgcaa cggctctata tcggcgaacc gagcgccgaa gccgttgccg
    27841 cgcaaatgcc ggatctgatt ttaattagcg caaccggcgg ggattcggcg ctggcactgt
    27901 atgatcagct ttccaccatc gccccgacat taatcatcaa ttacgacgac aaaagctggc
    27961 agtcgctgtt aacgcagctt ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga
    28021 ttgcacagtt tgataagcaa ctggcagcgg cgaaagagca aatcaaatta ccgccgcagc
    28081 cggtcactgc cattgtctat accgccgctg cacacagtgc caatctctgg acgccggaat
    28141 cagcacaagg gcagatgctg gaacaactcg gctttacgct ggcgaagttg cccgcagggt
    28201 taaacgccag ccaaagccag ggcaaacgcc atgacatcat tcagcttggt ggggaaaatc
    28261 tggctgctgg gttaaatggc gagtcactat tcctgttcgc cggtgatcag aaagatgccg
    28321 atgcgattta tgccaatcca ctgctcgcgc accttcccgc agtacaaaac aagcaggttt
    28381 atgcgctggg aaccgagacg ttccgtctgg attactacag cgccatgcaa gtgctggaac
    28441 gacttaaggc gctgttttaa gcattaactg tcagacgctg tcacctgcgg cggcgtctgg
    28501 cgaaaacgtc gcaactccac cagcaccagc aataacaaca cgccgataat caacaaacca
    28561 aaaccgctcg cgcttgcgga agcaaccggt gtcatcatcg cgcccaaacc acccagcagc
    28621 gccgcgccta tggcatcgcc cgtcacgttc tgcgccgtcc acaaaccgtt aatccgccct
    28681 aacatcgctt ccggggtttg cgtttgcagc attgtatatt gcagcaacga actaaccgcg
    28741 ctcagccagc cgaacagcgc cagacaaacc acgcctaaaa tccacatcgg catcaggcca
    28801 aacagaccaa tggcgaggaa cgatcccagc gtggagagca gcatcaataa ccctggtcgc
    28861 gcactatgtg ccagcttccc gctggttaac gcaccaatag ccgcgccgag cgggatcgcc
    28921 gcgtagagaa aaccaatctg tgccgctgac atctgccagt tgtcagccag cgccggatac
    28981 agtacccgca ccgcgctcgc catcgtcaat aaaccaccca gcagcgcaat cccgcccacc
    29041 agcgggctgg caagcagaaa acgaaatcct gccagtaatg atttcaacgg atgctcacgc
    29101 ggctgcggtg gcggtggcaa cgccggaagg cttaacaacg gtagcaaggt aataaacgtg
    29161 cccgccgccg ccagcccgta gttccaggct acgccaccgg tcgccagcaa taaaccgcca
    29221 atcatgggcg aaatcaccga ccccagacgc acggtcaaca tggtgatcgc cccggcctgc
    29281 attaagtttt cacgccctac cagtgcaggt gtcgccgcca gtagcgccgt aacgccaagt
    29341 gatgcgaaaa aaccatccca taaaccaagt aaatagattg ccagcaatga cggttcaggc
    29401 agcagtgcat taaggcacag tccaatgaag ccaatgccac aggtgccgcg cgccagcaaa
    29461 atcacttttt tgcgttcata gcgatccgcc agcacaccgc cgaccatcag gccaacaaac
    29521 atcgcgccgc cggtcagcgt caccgaaagc cccacctgcc aggtagaatg tgtcatcatc
    29581 tggatctgca ccggcaccgc gacgccgagc aaacccagag aaacaataga gatgaaacga
    29641 gcgaggaata ctgcgcgaaa cgccgggtgc gttttcaaca ggctgaggtt aagcagccag
    29701 gattgtttat tcattacaat gccttgccat caaatatttt aaatacctat tcttaggctg
    29761 cacatgctaa catatccaaa taagatcgat aacgataatt aatttcatta tcatggaagt
    29821 tcgtatgtct ggttctgttg ccgtgacacg cgccattgcc gtgcccggat tgctgttatt
    29881 actgattatc gcgacggcat taagtctgct cattggggca aaatcactcc ccgcttccgt
    29941 agtgctggag gccttctccg gcacctgcca gagcgccgac tgcaccatcg tgctcgacgc
    30001 gcgtctgccg cgtacccttg ccggtttact ggcaggcggc gcgcttggcc ttgccggggc
    30061 gttaatgcaa accctcaccc gtaacccact tgccgacccc ggcttgcttg gcgtgaacgc
    30121 cggagccagc tttgccattg tgctgggtgc ggcgctgttt ggttactctt ccgcacagga
    30181 acaactggcg atggccttcg ccggggcgct ggtggcctcg ttgattgttg cctttaccgg
    30241 cagccagggc ggcgggcagt taagtccggt gcgtttaacc ctggcgggcg tggcgctggc
    30301 ggcggtgctg gaaggactga ccagcggcat cgccctgctt aatcctgacg tctacgatca
    30361 actgcgtttc tggcaagccg gttcgctgga tattcgcaat ctacatacct taaaagtggt
    30421 gctgatcccg gtgctgatcg ccggagcaac tgcgctatta ctgagtcgcg cgctgaacag
    30481 tttgagcctc ggcagcgaca ccgcgacggc gctgggcagt cgcgtggcgc gcacacagtt
    30541 gattggtctg ctggcgatta ccgtgctttg tggtagtgcg acggcagtag ttggcccgat
    30601 tgcctttatt ggcctgatga tgccgcatat ggcgcgttgg ctggtgggtg ccgatcatcg
    30661 ctggtcgctg cccgtcacgc tacttgctac ccctgccctg ctgctgtttg ccgatatcat
    30721 cgggcgggtg attgtgcccg gcgaactgcg cgtttctgtg gtcagtgcgt ttattggtgc
    30781 accggtgctg atcttcctcg tgcgacgtaa aacgcgaggt ggtgcatgat ttacgtctct
    30841 cgccgattac tcatcacctg tttgctgctg gtttccgcct gtgtggttgc aggtatctgg
    30901 ggattacgca gcggtgccgt cacgctggaa acctcgcagg tattcgccgc gctgatgggc
    30961 gatgcgccgc gcagtatgac gatggtggtc accgaatggc gtttaccacg cgtgctgatg
    31021 gcgctgttga ttggcgcagc actgggcgtc agtggcgcga tttttcagtc gctgatgcgt
    31081 aacccgctcg gcagccctga cgtaatgggc tttaacaccg gggcgtggag cggcgtgctg
    31141 gtggcgatgg tgctgtttgg tcaggacctg acggctatcg cgctgtcagc aatggtgggc
    31201 ggcattgtca cttcgctgct ggtctggctg ctcgcctggc gcaacggcat cgacaccttt
    31261 cggttgatta ttatcggtat cggcgttcgc gccatgctgg tggcctttaa tacctggctg
    31321 ttgctgaaag cgtctttaga aacggcgcta acagcaggtt tgtggaatgc cggatcgctc
    31381 aacggcctga cgtgggcaaa aacctcgcct tccgcaccca tcattatatt gatgctcatt
    31441 gccgccgcct tactggtacg ccggatgcgc ttgctggaaa tgggcgatga taccgcgtgt
    31501 gcgctgggcg tcagcgtcga acgttcgcgt ctgttaatga tgctggttgc agtggtgctt
    31561 accgctgcgg caacagcgct tgccgggccg atttccttta ttgctttagt cgcaccgcac
    31621 attgcccgac gcattagcgg caccgctcgc tgggggctaa cccaggcggc gctatgcggg
    31681 gcgctgttac tgctggcggc cgatctctgc gcccaacaac tgtttatgcc gtatcaactt
    31741 ccggttggcg tcgttaccgt cagcctcggc ggtatttacc ttatcgtctt gttaattcag
    31801 gagtctcgca aaaaatgacc gaatcagtag cccgtttgcg cggcgaacag ttaaccctcg
    31861 gatatggcaa atataccgtt gcggaaaatc tgactgtaga aatacctgat ggtcacttca
    31921 cggcaattat cgggccaaat ggctgcggta aatccacgtt actgcgtacc ttaagccgcc
    31981 tgatgacgcc tgctcatggg catgtctggc tggatggcga gcacattcaa cattacgcca
    32041 gtaaagaggt tgcacgccgg attggtctgt tggcgcaaaa tgctaccacg ccgggcgata
    32101 tcaccgtgca ggagctggtg gcgcgtggac gttatccgca tcaaccgctg tttacccgct
    32161 ggcgcaaaga ggatgaagaa gcggtaacga aagcaatgca ggccacggga ataactcatc
    32221 tggcagatca aagcgtggat accctttctg gcggacaacg ccagcgcgcg tggatcgcga
    32281 tggtgctggc ccaggaaacg gcaattatgc tgctcgacga acccacgacc tggctggata
    32341 tcagtcatca gattgatttg ctggagttgt taagcgaact gaaccgcgaa aaaggctata
    32401 ccctggcggc ggtgctgcac gatcttaatc aggcctgtcg ttacgccagc catttgattg
    32461 cattgcggga agggaaaatt gttgctcagg gagcgccgaa ggagattgtc actgctgaac
    32521 tgattgagcg catttatggt ctgcgctgca tgatcattga cgatccagtt gccggaacgc
    32581 cgcttgtggt gccgctcgga cgaacggcac cttcaaccgc aaatagttaa actaagtggt
    32641 ctgccatcat ggcatcctgt tttctggatg ccatcgcata gcgcaacagc acgccaccac
    32701 aagccaccat cccgccgatc aacgcggaaa ggatcacaat aatcgcctta cccggaccgt
    32761 cttttttcac tggcaatgac gggcttaact gatatttaaa cggcgtaaaa ttcacatcgt
    32821 tgacatgtgc ttttgttaat tgctcgacaa gatactgccg attacgtaat tcaccgttca
    32881 gttccgcaac gtcagtgacc gctttttcta tttccagttt gcgttcaata ccgtctgcac
    32941 cgagagaaat agaaaaatcg gggtcatctt taacggcctg accattactg taaacgggct
    33001 ttttaattcc tgccgcgttg gcaatgtcga gtgaataatt gaggcgctga atgtttgcat
    33061 caagttgatt tttcgtttta atgcgatcct gagccagttt ttctttttca aactgggttt
    33121 tgatctccag tttattacgg acgttttcta gcgactcttt caccaccaac gtagagatat
    33181 aatcgatata cccgctcaaa acggtctgcg cctcttcact ggttggcgcg gtaaaactta
    33241 gcgtccagga ggtatacagt gacggttcat cttttttctt actggcattg tcatcaaccg
    33301 ctttcatttt ttcgctcaat gcgacaattg cgcgatgcaa atccagttcg tcgattttcg
    33361 cctcttttaa ttggtccatc acataaggtg atgaacgcag gtactcttcc agcaagctaa
    33421 ccgactgaaa cttcttgata aacaggttaa atgcttctgt acgatcaatt ttgatatcca
    33481 gatccagcac acgaagctta gtgaatgttt tctccaactc ttgccactga acaggttctg
    33541 gaggcgtgac aaccgccgcg ctggtccatt tttgcggcag gatgaaagag atcagcaagc
    33601 ctgcgcaggc aaacgcaaaa acgaccgcca tgaccgtttt tttggcccgc cataaaactg
    33661 agattagatt aagtaaatca atttcattat tactgggcga cgccagagga taatcgggaa
    33721 aatgagcgtc acttccctgt ttaatattca gtgatgacat ggggtataac ctgaaaatga
    33781 gtccaatagg gggaaatctc cacggcgctc actttaccag ttggtgagaa ttatccgaag
    33841 ctgaagtttg taaattcctt ccaatgaatg caacattcac tctatttaag agaaataacc
    33901 agcaaactta taaattaaac aaatgattaa cccataatta tttcctgtaa ttatgggtta
    33961 atagataatg ttaatttacc tgtttagcgt tgcgcgaata atcggcccaa ttttttcaaa
    34021 cgcccctgga gagataatat ccacatgcgc acaatcctga cgatagatat ccagttccgc
    34081 tatccacggc gaccaggcgc gttcgggact cataccttcc tgaagtgtgc gttcagcaac
    34141 aaacagcgtc gctttaccgt caaacggtac gctatgagca gtcgtcagca ggcgcacagc
    34201 atcagcgtag ttgccttcaa tggtggtaaa caactccgtt gaagtacttc cctgctgtgc
    34261 tgccaggaag gcctcgcgtt cgcggttaat ctccgccagc acttccgggt ccagaccatt
    34321 agcttctttt tcctgccagt tttgcgtttc tggcggccag gtatccagca agccaagaaa
    34381 tgccacctgt tcgccacggg cacgcagtcg cgccgcaata ccctgcgcca gcgtaccgcc
    34441 aagggaatac cccagcaggt aataagggcc atgcggttgt tgttcaagta acgttgccag
    34501 atgcgcttcg cagacttcat ccaggtttgc cgccgtctgc atggggccat tggggcgcgg
    34561 tgactgaatg ccgataatcg accattgtgg atcgagataa cgcgagagca cgctgaactg
    34621 ccaggcaaaa ccggacgcag gatggaaaca aaacagcgtc gggccattac cttcacgcaa
    34681 cggcagaatg gtttcgaatc ccatacgccg ggtgctgtct tcttcagcat caataatcgt
    34741 tgccagtttg gcgacagttg acgcgaccat cacttgcccc ggcgtcacct ggcgggcaac
    34801 ctgccgactt aactgcgctg ccagtttcat tgccagtagc gaatgaccgc caagcgcgaa
    34861 gaaatcagca tcggcatcct gcacgtcaca ccccagcaac gacgagaatg ccgcggcgat
    34921 aatcgtttca ctgcccgctt tcggcgcacg ccctggcgct tgtgccttca gttcaggcaa
    34981 cggtaaggct ttgcgatcca gcttgccgtt ggcgctaagt ggtaactgtg gaagttgcag
    35041 cagaaccacc ggtaccatat gtggtggcaa tgtttcacga agctgcgcct gcaatgcgct
    35101 ggtatccaac ggcaggcccg attgcgacac cagatagccc accaattgac gcgcatcacc
    35161 accggtggca gccgcctggt taatcacaca ggcgtgggta acggcttgtt cgacatccgg
    35221 cagcgcctgc atcacgcgat cgatttcgcc cagttcaata cgctgcccgc gaatttttag
    35281 ctgatcgtca ctgcgcccga ggtactccac cgcgccgtta tccagccagc gggcaacgtc
    35341 tccggtacgg tacatccgtt caccaggagc aaaaggatcg gcaataaagc ggctggcggt
    35401 cagatcgggt cgtccaagat acccctgcgc cagttgaata ccggtgagat agagatctcc
    35461 cgccacaccc ggcggcaccg gatgcatcat cgcatcaaga atacgcaggc ccgtgttcca
    35521 caccggataa ccaatcggca cactgctgcc gcgcacctgt gccagttcct cgccaaaagc
    35581 cggataccag ctgacatcta ccgccgcttc cgtcgggcca tatagattat gcaacggcgc
    35641 gccagttaac tgttgccatt cgcggcataa atcagccggt aaggcctcac cactacagaa
    35701 aacctgtttc aacgtcgcgc aactctggcg agcggtttgc ggcgtcagcg aggcaacaaa
    35761 tgccgccagc atcgacggca caaagtgcgt ggtcgttacg ccatattcgg caaagaattg
    35821 ctgcatagcg agcgggtcgc ggtgcgcttc cggttcagcc atcaccagtt ttgcccctgc
    35881 gataaacggc cagaaaaact cccacaccga gacatcaaaa ctgcacggcg ttttttgggc
    35941 aacgacatct tcgcctgtaa gcggataatg attttgcatc caaagcaggc ggttgacgat
    36001 agccgtctgc ccgaccatta cccctttcgg cctgccggtg gagccagagg taaagatgat
    36061 ataagccgtg tgatgcggtt gtgaaagttg cagcggcgca ctgccctgcg gtgtaagcgg
    36121 ggcgttatag caaaggttgg ttaaatctgg aacatcggca aagcgcggca gttgatcgtc
    36181 ggtggtgatt aacagcgacg gacgcgcatc ttctagcatc attttcaggc gatcgtccgg
    36241 ataaccggta tccagcggta gccaggccgc acctgcttca acaatcgcat gtagcgccag
    36301 ggtcaaaaag accgagcgcg gtagcgccac cgctacgctg tcgcccggtt taacgccgcg
    36361 ctcacgcagc agattcgcca gcgccaccac ctgctcacgc atttcccgat agctgaactg
    36421 gtaatgcgca tctgccagcg ccggagcatc cggagttttt gccgcttgtt ctgccaccag
    36481 agcgctaagc gtggtttctg gaatctcaac ctgagtggcg ttgatctgcg ccagctgcgc
    36541 gtattcacct ggcagcataa tatcgacatc accgcacaac agcgccggat ctgcggcgaa
    36601 ctgggcaatc agcattttca ggcgttcagc atgctggatt aacgttggct cagcgtaacg
    36661 ctgtttattg gcgaggatct caatactcaa atcaccgtgt acatccggga acagggccag
    36721 ttcaaggtca ttaaccggac cggttgccag ggtatgggtt tgcgcctgaa catcaggaat
    36781 atccagttgg taatcaaata ccttgatatt gagtaccgga ccaaacagcg gttcatcacc
    36841 tgccgctcgc ccgctgtcac ggacaatttg ttcggcatcg taacgttgat gacgacgcat
    36901 ttttttcagt tgtgctgcca gtcgggttgc cagttccggc agcgtttctt gcgccgcaat
    36961 gtgaataccc aacggcaaaa cgttgagcac gggtccggta gccgtcagcg ccgccgagcc
    37021 cagtcgacgc ataaagataa atccggcggc gtagtccata cgattgcaca atcgccccag
    37081 ccacaaggct gccagcgcaa gggctaaatc ggtacgctgc acacctgaaa gttgcgtagc
    37141 cagctggcgg aattccccgt cggtaaattc cagtttcagg cgcagaatat ctgccgaggc
    37201 gctgcgcccc ggtaaaggtg ccggagaaag tgacgcgggc ggcggcagtt gacgacgctg
    37261 ttctgcccag aatgccgcat cacgctgcca ggcttcgctt tcgcggtatt gctggtactc
    37321 ttccactaca tcagcgaaag gcgtaaacgg cgaagcaggc gttggttcgc cacgcagcca
    37381 tgtgcagtaa atattggcga tctggcgagt aatggccggg aaactgaagc catcgaccag
    37441 caaatggtga taacgctgat accagtacca gcggttatcc gcaacatgta tcagctgatg
    37501 aaagaccagt ggtttaccgc tatcgacgcg caaatcttgt tgcaaatccg ccagcattaa
    37561 tgcctgcgca gtaccgtgcg gatcaatatt ggttcgcagg tcgataattt ctggcagttc
    37621 gaatatcagc gcatcatcga cccactgcca gacttcgccg ttatcttccg taaaacgcat
    37681 ccgcaacgta tctgcttgcg ctagtccgac aaccaccgcg cgggccagta atggcgcatc
    37741 aacatctcca gttaactcaa cgtaatgcgc cacgctccag gcggagggta aatccgacag
    37801 tttttctgcc atccagatgc cgggctgtgc ggcgaccaaa ggtaaatgct ggctcattgt
    37861 gcctcctgca actgggtgaa attggccggt gtcagagtac gccagtggac ttccagccac
    37921 tgctggcagg acgcctgtga ctgcggctga cacacaatgt cccagcctgc cggtaaggcg
    37981 cattgttgcg gccacagact gaattgcccc tgcgcattgc gcaaaatgta aaacgctccc
    38041 tgcggatcat cgaagggatt actgaatgcc atattcaact cctgtcatgg aaaagtggtt
    38101 gccagaggtc gattagcccc tgcatcaagc caccgcgcca acaaagcgca tcatgtccgc
    38161 cgtcaacctg acgccagaaa atggattctt ttatggggtg taattgcgca tacagcgcct
    38221 gattggcccg cattatcatc ggctcgcgaa tacccgcttc cagcacaatg cgcagacctt
    38281 cggcactaac ttgaccagct ttaagttgct caagtaaaac accttcttgc tgcccgcccc
    38341 gatgcggcca ccagtacgat cctgactggc ttaatacaca gccaaagcgt tcaggccagt
    38401 gcagtccggc atacagcgcg gaaagtccac caaaactctg cccggcgacc acggtgcgat
    38461 cggcgcgatc gctaaaaggg gcaatagctt tcaccagggg taataactct tgctgtactg
    38521 ccagccagaa atccgcatta cacggcagtt cgtgagcgcg gtgcgtagtg tcgatagcgt
    38581 cgatcaacac atacacggcg ggaggaagtt gctgacgatg ggtcaacgaa gtcagcacag
    38641 gccagacagg catactctgc gcccaaaatt cgccatcgag caaaactgcc agcgggcgtt
    38701 ctcctgctgt tgcatcgcca gtggtaaaaa tccatacacg gcgtgaattt ttcaaccgtt
    38761 cacttttcca gataatttct ttggcaggcg tttctggcgc ttgcggacaa tcccatcccg
    38821 gttgcagagg cgcttgcggc atttcgagtg cagaaacagc gtgccctcgc ccgcctttcc
    38881 agctttgtgg attcagcgga tcggctattg cctggggtaa tagttttcgc cagccttcac
    38941 gtaattcgag gcgatcgggg gatggtgcag aaaaaatgtc atcgcgttcg gtgggaataa
    39001 aacagtagct gccgcgccag ttggcattga gttgtgtcgt ccactgccag acgttggtgc
    39061 ctgcaattcg ctgcatcgac tggggctggc tgttctgatg gtgatcggtc acaccagtga
    39121 tgtagaccca tacgcgcttt atcgtcgagt attcttcaga accttgggga tcacgccacc
    39181 agaaagtgac ctcaaacatt tcgtcattca gacgctgcca ttccgggcca tgtttcgact
    39241 gccaccagct ctcacttcct acttttaacg ccgtcacacc ataaccccat gtttactgtg
    39301 caatttttca ttgattgcag aaatatattg ataatattat tgataactat ttgcatttgc
    39361 aatagcgtaa tggcgcgccg tgggaagcgc ggacattaat taaccaactg cactgcgtgt
    39421 ctttcaggat caaaggtttt cgcggtagcg ggatgcgtcg tattgatgac gaccatgccc
    39481 gacagttgca attcgtggca aaaatgcagg aataaaacaa tgaacaagaa gattcattcc
    39541 ctggccttgt tggtcaatct ggggatttat ggggtagcgc aggcacaaga gccgaccgat
    39601 actcctgttt cacatgacga tactattgtc gttaccgccg ccgagcagaa cttgcaggca
    39661 cctggcgttt cgaccatcac cgcagatgaa atccgcaaaa acccagttgc ccgcgatgtg
    39721 tcggagatca tccgtaccat gccaggcgtt aacctgaccg gtaactccac cagtggtcag
    39781 cgtggtaata accgccagat tgatattcgc ggcatgggtc cggaaaacac gctgattttg
    39841 attgacggca agccagtaag cagccgtaat tcggtgcgtc agggctggcg tggtgagcgc
    39901 gatacccgtg gtgatacctc ctgggtgcca cctgaaatga ttgaacgtat tgaagttctg
    39961 cgtggtccgg cagctgcgcg ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt
    40021 accaaaaaag gcagtggtga gtggcacggc tcctgggatg cttatttcaa tgcgccagaa
    40081 cataaagagg aaggtgccac caaacgtacc aacttcagcc tgaccggtcc gctgggcgac
    40141 gaattcagct tccgcttgta tggcaacctc gacaaaaccc aggctgacgc gtgggatatc
    40201 aaccagggcc atcagtccgc gcgtgccgga acgtatgtca cgacgttacc agccgggcgc
    40261 gaaggggtga tcaacaaaga tattaatggc gtggtgcgct gggacttcgc accattgcaa
    40321 tcgctggaac tggaagcggg ttacagccgc cagggtaacc tgtatgcggg cgatacccag
    40381 aacaccaact ctgacgctta cactcgctcg aaatatggcg atgaaaccaa ccgcctgtat
    40441 cgccagaact acgcgctgac ctggaacggt ggctgggata acggcgtgac caccagcaac
    40501 tgggtgcagt atgaacacac ccgtaactcg cgtattccgg aaggtctggc gggcggtacc
    40561 gaagggaaat ttaacgaaaa agcgacacag gatttcgtcg atatcgatct tgatgacgtg
    40621 atgctgcaca gcgaagttaa cctgccgatt gatttcctcg ttaaccagac gctgacgctg
    40681 ggtacggagt ggaatcagca acggatgaag gacttgagtt ccaataccca ggcgctgacc
    40741 ggggcgaaca ccggcggcgc tattgatggt gtgagtgcca ccgaccgtag cccgtattca
    40801 aaagcagaaa ttttctcgct gtttgccgaa aacaacatgg agctgactga cagcaccatc
    40861 gtaacgccgg ggctgcgttt cgatcatcac agtattgtcg gcaataactg gagcccggcg
    40921 ctgaacatat cgcaaggttt aggcgatgac ttcacgctca aaatgggcat tgcccgtgct
    40981 tataaagcac cgagcctgta ccagactaac ccgaactaca ttctctacag taaaggtcag
    41041 ggttgctatg ccagcgcggg cggctgctat ctgcaaggta acgatgacct gaaagcagaa
    41101 accagcatca acaaagagat tggtctggag ttcaaacgcg acggctggct ggcgggcgtc
    41161 acctggttcc gtaacgatta tcgcaataag attgaagcgg gctatgtggc tgtaggacaa
    41221 aacgcagtcg gcaccgatct ctatcagtgg gataacgtgc cgaaagcagt ggttgaaggt
    41281 ctggaaggat cgttaaacgt accggttagc gaaacggtga tgtggaccaa taacatcact
    41341 tatatgctga agagtaaaaa caaaaccacg ggcgaccgtt tgtcgatcat cccggagtat
    41401 acgttgaact caacgctgag ctggcaggca cgagaagatt tgtcgatgca aacgaccttc
    41461 acctggtacg gtaagcagca gccgaagaag tacaactata aaggtcagcc agcggttggc
    41521 ccggaaacca aagaaatcag tccttacagc attgtgggtc tgagcgcgac ctgggatgtg
    41581 acgaagaatg tcagtctgac cggcggcgtg gacaacctgt tcgacaaacg tttgtggcgt
    41641 gcgggtaatg cccagaccac gggcgatctg gcaggggcca actatatcgc gggtgccggg
    41701 gcgtatacct ataacgagcc gggacgtacg tggtacatga gcgtaaacac tcacttctga
    41761 tgctaacgtc agattgttga caaagtgcgc gtcgttcatg ccggatgcgg cgtgaacgcc
    41821 ttatccggcc tacaaaaatc ttgccaattc aatatattgc aggaccatgt aggcctgata
    41881 agcgtagcgc atcaggcagt ttagcgtttg tcatcagtct ctaatatggt cgacatgaaa
    41941 actacgcata cctccctccc ctttgccgga catacgctgc attttgttga gttcgatccg
    42001 gcgaattttt gtgagcagga tttactctgg ctgccgcatt acgcgcaact gcaacacgct
    42061 ggacggaagc gcaaagccga acatttagcc gggcggatcg ctgcaattta tgcgctgcgg
    42121 gaatatggct ataaatgtgt gcccgcaatc ggcgagctac gccaaccggt gtggcctgcg
    42181 gaggtatacg gcagtattag ccactgcggg gctacggcat tggccgtggt ttcccgtcaa
    42241 ccgattggcg ttgatatcga agaaattttt tctgcgcaaa cagcgaccga attgacagac
    42301 aacattatta caccagcaga acacgagcga ctcgcagact gcggtttagc cttttctctg
    42361 gcgctgacac tggcattttc cgccaaagag agcgcattta aggcaagtga gatccaaact
    42421 gatgcaggtt ttctggacta tcagataatt agctggaata aacagcaggt catcattcat
    42481 cgtgagaatg agatgtttgc tgtgcactgg cagataaaag aaaaaatagt cataactctg
    42541 tgccaacacg attaattgac aacatctggt acgattcgcc cgcagccatc actgaccgcg
    42601 ggcgaaagtg taaagcaggt gccttaccac tctgacctga caaccggata tgcggggatt
    42661 gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat
    42721 tacgttcctt tacggtgaac tcacacagcg agtctccgac cagaagcgta aatcccagta
    42781 ccgtcaaaca cagcactatg actgccacaa gggcatattt cgtcagcatg tttatattgc
    42841 ctccatgcga agaagggact accatccgag ttgttgaggc tcggagtggc agccccagga
    42901 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagttaac ctgaaaccgg
    42961 aaagcttcag gcacccgccg ttatcttacc cttcctcaac aaaatagctg tttatttata
    43021 cagtgaatcc tgtttcggac ttctgtcggc aaaactcatc attgctctta aaacgaaatc
    43081 gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac catcctgacc
    43141 tgacaaccgg atatgcgggg attgctcccc gcacaacggc tacttcttcg gttcgtaagc
    43201 gagaacaacc ttaaactcaa tattacgttc ctttacggtg aactcacaca gcgaatcccc
    43261 gaccagaagc gtaaatccca gcaccgttaa acacagcact atgactgccg caagggcata
    43321 tttcgtcagc atgtttatat tgcctccatg cgaagaaggg actaccatcc aaattgttca
    43381 ggtttgggta ggtagcccca gggttgatag aaatatcgtc ctggggcttc atctttccag
    43441 acctcagagt aaacctgaaa ccggaaagct tcaggcaccc gcagttatct tacccttgct
    43501 tagcaaaata gctgtttatt tatacagtaa gtcctgtttc ggacttctca ctccccattt
    43561 gatcaactga agctgaaccc gtttttttct gcatgcctca atcgtcaaaa ttcttcgcca
    43621 ccagccagcc gtgccataac ttgtcacatt gcaaaagtag ccaaattgct tcagtcacca
    43681 tcaccaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc cgacttctat
    43741 actgaataga aaacgccaac ttaagagaaa cctatgccat tacccgattt tcatgtttct
    43801 gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac
    43861 ttaagccagg actcttcaat gctgattgac gcggttaaaa atgagatcac ggccggagag
    43921 gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc
    43981 aaccaggctg ccgggcagtt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac
    44041 catcatctgg aaatttgcgg cggtggcacg cacccgtttc agaaatggca gcgtcaggag
    44101 gtatgcgaca acgaacgcta tcaacgaacg ctggaaaact ttggctatct catccagcag
    44161 gcgaccgttt ttggtcagca tgtccatgtt ggctgcgcca gtggcgatga cgccatttat
    44221 ttgctgcacg gcctgtcacg gtttgtgccg cacttcatcg ccctttccgc cgcgtcgccg
    44281 tatatgcagg gaacggatac gcgttttgcc tcctcacgac cgaatatttt ttccgccttt
    44341 cccgataatg gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc
    44401 tgcctgagtt acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc
    44461 agtcctcatt ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac
    44521 gcggtaaata tggcgggatt aattcaggcc accgcccact ggttactgac ggaacgcccg
    44581 tttaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgccgc
    44641 tatgggctgg aaggcgtcat taccgatccg cacactggcg atcgtcgacc gctaacggaa
    44701 gataccttgc gattgctgga aaaaatcgcc ccttccgcac ataaaatggg tgcatcgagc
    44761 gcgattgagg ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc
    44821 gatttcgtcg ccgatggtgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg
    44881 gccggtgact aagtagggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac
    44941 ttacatttaa caatgttatg aaacaccctt tagaaacctt gactaccgca gcaggcattt
    45001 tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg
    45061 cacaaaaact ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt
    45121 tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc
    45181 gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt
    45241 gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg
    45301 aaacgcctgg cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc
    45361 gatgttttta aaattggcgg caaaattttt atgctgtcgt cggagctgcg cggcgtcccc
    45421 tttatcaatc tgaagtccga tccacaaaaa tccctgttaa atcagcagat atacccgagc
    45481 attaagccag ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa
    45541 atctccgaag cgttaattcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt
    45601 ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt
    45661 tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca
    45721 catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt
    45781 gatgccagta aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac
    45841 agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt
    45901 aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg taaaatactt
    45961 gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag
    46021 ctggttgttg accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg
    46081 aacgataaag gtcgcaagtt cttcgctgat atgcaccgta aagatctgca tgatgatgca
    46141 gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg
    46201 ctgggtctgg acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt
    46261 ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt
    46321 gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacatcac cttaaccgaa
    46381 gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg
    46441 cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg
    46501 atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg
    46561 tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg
    46621 tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca
    46681 ttacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata
    46741 ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg
    46801 cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt
    46861 tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg
    46921 ggattaaact gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc
    46981 agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggttt
    47041 ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga
    47101 aactgggcga ctggctggag atgccgaaat acggcgcgga tggcgcggtg atcgatattg
    47161 ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt
    47221 ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta
    47281 ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc
    47341 aacgtctgaa taaagcgcac ttgttaaaac cttatttaac cagccgccat caggaaatta
    47401 atgagtggaa tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc cgaatgacca
    47461 atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta
    47521 aagatatgac cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga
    47581 tctacgcgtt tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat
    47641 tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg
    47701 gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat
    47761 cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg
    47821 cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa
    47881 gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg caatgcaaag
    47941 atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat
    48001 cgctgcacga aaacgcagat gcgccagaca gatcataatc cagttcaaca gcagcgttgc
    48061 taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaatac
    48121 caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct
    48181 gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct
    48241 gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag
    48301 cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt
    48361 ggatttcact tccacccacg gatagagcgc cagtaaaacc accagtgaac cgatgtaaaa
    48421 cagcaggatg cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg
    48481 cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc
    48541 cagcgacaaa atcagcccat tccagccggt ggcgaagaaa ccaccgtagc gccagaggtt
    48601 gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat
    48661 cataccgatg atcgccagca ctttaatcag cgcaaaccag aactcggttt caccatataa
    48721 gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca
    48781 cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat
    48841 tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc
    48901 ccagtattta taggcaaagt gggcaaatga accggatacc ggctcctcaa ccaccatttc
    48961 gccaagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac
    49021 agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc
    49081 gccacccaac gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc
    49141 ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt
    49201 gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc
    49261 acacggttaa acggggtatc ctgcttttat ttccgtaccc gatgtcggtg cagccacatc
    49321 agcttatacg ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca
    49381 atcatcggtg cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc
    49441 ggcagcagac cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc
    49501 gcgccgtgat ataacgcctc atccagcttc tgctcgctga atgtttgtgg attattcaac
    49561 gacggcacgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg
    49621 gcgaccccgg cgagggcgat aaagccagtg cccgtcgcca cggaaagatg aaagcccatc
    49681 caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac
    49741 gcttcgccca cccgacggaa cgccagatac aacagcacga agataatcat caacgtcatc
    49801 ggcaccatga gtttaagctt atggttggcg cgctccagta gctcgaactg cccggagaat
    49861 gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga
    49921 accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca
    49981 ttctcggttt tcagcatcga cggtccggta gagactttaa tgtcggccac gtctgccagg
    50041 gtgatttgct gcttcatcgg cgtcaggatc ggcagctggc gcagtgcctg cggactatcg
    50101 cgccagcttt gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg
    50161 ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata
    50221 ccgtaacgtg cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc
    50281 cgctcggcaa gcgcagaagc tacgcctggc accgttcgcg ctacttcttc aatttgctca
    50341 gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta
    50401 atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc
    50461 cccggcagcc gcacggtgtt atccagttcc tcaatgattt tgtccatcgt catgcctggc
    50521 cgccactgct cctgcggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa
    50581 tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc
    50641 ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc
    50701 agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca
    50761 actttattga gcggccagag aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt
    50821 ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg
    50881 agcgggttac tgctttccgg cggaattttg ccacggatcc agtagcccat caggatcggg
    50941 atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccaac
    51001 gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg
    51061 ataatcagca gactgataaa tagcgccggc cccacttcaa cagacgcatc ggtgatcacc
    51121 tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc
    51181 cgtttatgtg cattttcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc
    51241 gcaatgccac ccaacgacat aatattggca ttcagtccct ggaagtgcat gacaataaaa
    51301 gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag
    51361 agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg
    51421 ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct
    51481 tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg
    51541 gcgtttttgc cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttagt
    51601 tcggcaatgc cccggcgcat ctccgggcca atctggacct tcgcaacatc gcgcagataa
    51661 acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc
    51721 agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga cgaaccgccc
    51781 gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc
    51841 gccaggcgct ggggatcgat aaccacctga tactctttca ccacaccgcc caccgacgcc
    51901 acttccgcaa cgtcagggat ggttttcagc tcatatttga gaaaccagtc ctgtaatgag
    51961 cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag
    52021 ccaacacccg tggcatctgg ccccagctcg gcgctgactc ccgcaggcag cttaccctgt
    52081 acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt gccatcttcg
    52141 aaaatgacat acacataaga gtcgccaaac tgtgagaaac cgcgcacagt cttcgcgcca
    52201 ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac gatttgtggt
    52261 gcctgaccgg gatagctggt tttaataata acctgcacat cggagagatc cggcagcgca
    52321 tccactggcg tattaatgat ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc
    52381 agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc
    52441 ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg
    52501 cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc gccagaccag
    52561 agcgtaatgc ggtgacgcct tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc
    52621 gcccgtcggc atcaacggta atcacccgct gttcgctgcc ggtatcaatc agcgcttgtg
    52681 acggaatgag cagcatcggt tcgctggcgg tgttgagttg caaccaggcg ttcatgcccg
    52741 gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg
    52801 catccacgcc aggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgcgccg
    52861 gaacggtgag ggtaaactgc gaggcatctt tcaccagcca ggcgatagac tccgggatcg
    52921 cagcagtgac ccacaccggg tccataccct gaattttcgc taccacgtta tctttggcga
    52981 tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg
    53041 taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc
    53101 ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc
    53161 gcagcagtaa atactcactc tgcgcttcca cccagtcagg aatggtcagg tcgagaagcg
    53221 gtgtgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg
    53281 cagcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac
    53341 tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg
    53401 tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca
    53461 ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta ttggggtaca
    53521 tcgggtcgta ccagaataag attttacgtt ctgcggtcga cgttttttct gcgggtggtt
    53581 cagcctttgc aaaccaggta aaacctgccg cagaaataat accgcccgcg atcatgctgc
    53641 cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat
    53701 cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccagttt
    53761 taatttcact cattttcgtc tgcggagtga tggtaaagcg catggtcatc tccggccagt
    53821 tcacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga tcaataccct
    53881 ttaccacgcc agtggcgcta ataacctgcg gttgtacttc gctcatggtt tcatgatgat
    53941 gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca
    54001 gtgctttttt catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa
    54061 ggcggtatac aaagaaattt cgttaacctg acgggcataa ttcaggtcga gtaaagtttg
    54121 tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg
    54181 ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat aacgctgctg
    54241 ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca cttctttaaa
    54301 ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa tttcggcgat
    54361 atccagattg gcttggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa
    54421 attccacatc ccgctgctag cgttaaataa tgaagacaga tcgctgctgg cggttgatat
    54481 tccgctggtc aggctgatag acgggaaaaa tgccgcacgc gcagcgccaa tattggcatt
    54541 agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga
    54601 cgacaatccc gccggtaatt taacgctttg caggctgtcg ctgtttactg tctgcgcttg
    54661 cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc
    54721 cccctgacgt ttagcgatgt cgctgcgggt actttctatc accccgcgag cctgttccag
    54781 cgccagaaca ttactgctac ctgtcaacag ttgtttttcg acaaacgcat atgactgctg
    54841 ataattacgc agcgtttctt cggctatttg caattgcgca tacgccagtt gctgattgaa
    54901 atagctttgc gcgacattag aaaccagcag aatatgcacc gcgcgctgag cttcctcagt
    54961 ggctaaataa ttttgtcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag
    55021 gtcaaagctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat tccctttaag
    55081 attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt cggcatcggt
    55141 cagacgatat tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccgatt
    55201 attcaccagc gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca
    55261 gcccgcgttc tgatagttat ctgctgcgtt aaccaggccg ttctggctga gtgagaactg
    55321 ctgcggcacg ggcattgccg gacgctgata atccggtgcc agtgaacaac cggttagcgc
    55381 aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat
    55441 tttatgccgc caactttact cgccaggctc tgattttccg gtgacagaaa aatgacaaaa
    55501 ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca
    55561 acatgcggag gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac
    55621 ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggttgaat
    55681 ggctaccatc tggcgatgac cggtgattat gatctgataa tcctcgatat tatgctgccg
    55741 gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg ccaataaagg gatgccgatt
    55801 ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggcg
    55861 gatgactatc tggtgaagcc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc
    55921 ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc
    55981 gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagag
    56041 tttactctgc tggagttttt cctccgccat cagggcgaag tgctgccccg ctcgcttatc
    56101 gcctcgcagg tgtgggacat gaatttcgac agcgacacta acgccattga tgtagcggtg
    56161 aagcggctgc gcggcaaaat cgacaacgac tttgagccaa agctaattca gaccgtgcgc
    56221 ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc
    56281 gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcagcgt ttttcgcctt
    56341 tgcatggatc atgatccact cagtaaaagt gcattttgcc gagcaggata ttaatgattt
    56401 aaaagaaatt agcgccacgc ttgaacgggt acttaatcat cctgacgaga cgcaagcccg
    56461 acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga tctccctggc
    56521 agatagccat ggtaaaacgg tgtatcactc ccccggtgcg ccggatatcc gcgagtttac
    56581 gcgtgacgcc atacccgata aagacgcgcg gggcggcgag gtgtatctcc tttccggccc
    56641 gacgattatg atgccagggc atggtcacgg gcatatggaa cacagcaact ggcggatgat
    56701 taacttgtcg gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct
    56761 ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc
    56821 atcgatcatc agcatcctga tcgtctttat cgtgctattg gcggtacata aaggtcacgc
    56881 gccgatccgc agcgtcagcc gtcaaatcca gaatattacc tcgaaagatc tcgacgttcg
    56941 ccttgacccg cagaccgtgc ccattgagct ggaacagctg gtactgtcgt tcaaccatat
    57001 gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg atatcgctca
    57061 cgaaattcgc acgccgatta ccaatctcat cacgcaaacg gaaatcgccc tcagccagtc
    57121 tcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat
    57181 ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc
    57241 cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc
    57301 gttagcggaa gatcgcggcg tggagttgcg atttgttggc gacgaatgtc aggttgcggg
    57361 cgatccgctg atgctgcgtc gggcgttaag caacctgctt tctaatgccc tgcgttatac
    57421 gccgcccgga gaggcaattg tagtgcgctg ccagacggtc gatcaccagg tgcaagttac
    57481 cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt
    57541 ctaccgcgtt gacccttccc gccagcgaaa aggcgaaggt agcggcattg ggctggcgat
    57601 agtgaaatcg attgttgtcg cgcataaagg cacggttgca gtaacgtcag atgcgcgggg
    57661 gacaaggttt gtgattatgc tgccggagag agagtgatta ttaagttcaa attcagggcg
    57721 tggtgcatca cctcctggta ttgttgatca acaaattaga gaaaaactta ttaaacatat
    57781 gaaatatcga aggcttttat ttttcaaagt ttc
//
LOCUS       DAZFYC010000014        57679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFYC010000014 DAZFYC010000000
VERSION     DAZFYC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.014"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(158..3682)
                     /gene="nifJ"
                     /locus_tag="ACHKW5_001030"
     CDS             complement(158..3682)
                     /gene="nifJ"
                     /locus_tag="ACHKW5_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO7510464.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            3956..4222
                     /gene="ydbJ"
                     /locus_tag="ACHKW5_001031"
     CDS             3956..4222
                     /gene="ydbJ"
                     /locus_tag="ACHKW5_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO7510465.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4219..4641)
                     /gene="hslJ"
                     /locus_tag="ACHKW5_001032"
     CDS             complement(4219..4641)
                     /gene="hslJ"
                     /locus_tag="ACHKW5_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO7510466.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4752..5741)
                     /gene="ldhA"
                     /locus_tag="ACHKW5_001033"
     CDS             complement(4752..5741)
                     /gene="ldhA"
                     /locus_tag="ACHKW5_001033"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7510467.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5949..8588
                     /gene="ydbH"
                     /locus_tag="ACHKW5_001034"
     CDS             5949..8588
                     /gene="ydbH"
                     /locus_tag="ACHKW5_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO7510468.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            8585..8770
                     /gene="ynbE"
                     /locus_tag="ACHKW5_001035"
     CDS             8585..8770
                     /gene="ynbE"
                     /locus_tag="ACHKW5_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO7510469.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            8775..9104
                     /gene="ydbL"
                     /locus_tag="ACHKW5_001036"
     CDS             8775..9104
                     /gene="ydbL"
                     /locus_tag="ACHKW5_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO7510470.1"
                     /translation="MMKKTLLLCAFLVGLVSSNIMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(9276..10181)
                     /gene="feaR"
                     /locus_tag="ACHKW5_001037"
     CDS             complement(9276..10181)
                     /gene="feaR"
                     /locus_tag="ACHKW5_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO7510471.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            10417..11916
                     /gene="feaB"
                     /locus_tag="ACHKW5_001038"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             10417..11916
                     /gene="feaB"
                     /locus_tag="ACHKW5_001038"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO7510472.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            complement(11974..14247)
                     /gene="tynA"
                     /locus_tag="ACHKW5_001039"
     CDS             complement(11974..14247)
                     /gene="tynA"
                     /locus_tag="ACHKW5_001039"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO7510473.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPTKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(14495..16540)
                     /gene="paaZ"
                     /locus_tag="ACHKW5_001040"
     CDS             complement(14495..16540)
                     /gene="paaZ"
                     /locus_tag="ACHKW5_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO7510474.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELLPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDITPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQTLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            16825..17754
                     /gene="paaA"
                     /locus_tag="ACHKW5_001041"
     CDS             16825..17754
                     /gene="paaA"
                     /locus_tag="ACHKW5_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO7510475.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            17766..18053
                     /gene="paaB"
                     /locus_tag="ACHKW5_001042"
     CDS             17766..18053
                     /gene="paaB"
                     /locus_tag="ACHKW5_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO7510476.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            18062..18808
                     /gene="paaC"
                     /locus_tag="ACHKW5_001043"
     CDS             18062..18808
                     /gene="paaC"
                     /locus_tag="ACHKW5_001043"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO7510477.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            18823..19320
                     /gene="paaD"
                     /locus_tag="ACHKW5_001044"
     CDS             18823..19320
                     /gene="paaD"
                     /locus_tag="ACHKW5_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO7510478.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            19328..20398
                     /gene="paaE"
                     /locus_tag="ACHKW5_001045"
     CDS             19328..20398
                     /gene="paaE"
                     /locus_tag="ACHKW5_001045"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO7510479.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            20395..21162
                     /gene="paaF"
                     /locus_tag="ACHKW5_001046"
     CDS             20395..21162
                     /gene="paaF"
                     /locus_tag="ACHKW5_001046"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO7510480.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            21162..21950
                     /gene="paaG"
                     /locus_tag="ACHKW5_001047"
     CDS             21162..21950
                     /gene="paaG"
                     /locus_tag="ACHKW5_001047"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO7510481.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLECDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            21952..23379
                     /gene="paaH"
                     /locus_tag="ACHKW5_001048"
     CDS             21952..23379
                     /gene="paaH"
                     /locus_tag="ACHKW5_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO7510482.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            23369..23791
                     /gene="paaI"
                     /locus_tag="ACHKW5_001049"
     CDS             23369..23791
                     /gene="paaI"
                     /locus_tag="ACHKW5_001049"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO7510483.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            23791..24996
                     /gene="paaJ"
                     /locus_tag="ACHKW5_001050"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             23791..24996
                     /gene="paaJ"
                     /locus_tag="ACHKW5_001050"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO7510484.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            25023..26336
                     /gene="paaK"
                     /locus_tag="ACHKW5_001051"
     CDS             25023..26336
                     /gene="paaK"
                     /locus_tag="ACHKW5_001051"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO7510485.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            26437..27387
                     /gene="paaX"
                     /locus_tag="ACHKW5_001052"
     CDS             26437..27387
                     /gene="paaX"
                     /locus_tag="ACHKW5_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO7510486.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            27369..27959
                     /gene="paaY"
                     /locus_tag="ACHKW5_001053"
     CDS             27369..27959
                     /gene="paaY"
                     /locus_tag="ACHKW5_001053"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO7510487.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHMGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            28191..29051
                     /gene="pdxI"
                     /locus_tag="ACHKW5_001054"
     CDS             28191..29051
                     /gene="pdxI"
                     /locus_tag="ACHKW5_001054"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO7510488.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            29115..31421
                     /gene="ydbD"
                     /locus_tag="ACHKW5_001055"
     CDS             29115..31421
                     /gene="ydbD"
                     /locus_tag="ACHKW5_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO7510489.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            31592..32197
                     /gene="ynbA"
                     /locus_tag="ACHKW5_001056"
     CDS             31592..32197
                     /gene="ynbA"
                     /locus_tag="ACHKW5_001056"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7510490.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            32197..33093
                     /gene="ynbB"
                     /locus_tag="ACHKW5_001057"
     CDS             32197..33093
                     /gene="ynbB"
                     /locus_tag="ACHKW5_001057"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7510491.1"
                     /translation="MLEKSLATLFALLILATLINHFLVWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            33109..34866
                     /gene="ynbC"
                     /locus_tag="ACHKW5_001058"
     CDS             33109..34866
                     /gene="ynbC"
                     /locus_tag="ACHKW5_001058"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO7510492.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HCGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTMAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            34856..36172
                     /gene="ynbD"
                     /locus_tag="ACHKW5_001059"
     CDS             34856..36172
                     /gene="ynbD"
                     /locus_tag="ACHKW5_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO7510493.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(36223..36828)
                     /gene="azoR"
                     /locus_tag="ACHKW5_001060"
     CDS             complement(36223..36828)
                     /gene="azoR"
                     /locus_tag="ACHKW5_001060"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO7510494.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            37029..40931
                     /gene="hrpA"
                     /locus_tag="ACHKW5_001061"
     CDS             37029..40931
                     /gene="hrpA"
                     /locus_tag="ACHKW5_001061"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO7510495.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            41203..42003
                     /gene="ydcF"
                     /locus_tag="ACHKW5_001062"
     CDS             41203..42003
                     /gene="ydcF"
                     /locus_tag="ACHKW5_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO7510496.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            42200..43639
                     /gene="aldA"
                     /locus_tag="ACHKW5_001063"
     CDS             42200..43639
                     /gene="aldA"
                     /locus_tag="ACHKW5_001063"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO7510497.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(43681..44682)
                     /gene="gap"
                     /locus_tag="ACHKW5_001064"
     CDS             complement(43681..44682)
                     /gene="gap"
                     /locus_tag="ACHKW5_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7510498.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            44871..45401
                     /gene="cybB"
                     /locus_tag="ACHKW5_001065"
     CDS             44871..45401
                     /gene="cybB"
                     /locus_tag="ACHKW5_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO7510499.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            45646..45819
                     /locus_tag="ACHKW5_001066"
     CDS             45646..45819
                     /locus_tag="ACHKW5_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510500.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            45845..45913
                     /gene="yncP"
                     /locus_tag="ACHKW5_001067"
     CDS             45845..45913
                     /gene="yncP"
                     /locus_tag="ACHKW5_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO7510501.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(45891..46040)
                     /gene="hokB"
                     /locus_tag="ACHKW5_001068"
     CDS             complement(45891..46040)
                     /gene="hokB"
                     /locus_tag="ACHKW5_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO7510502.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            46439..48079
                     /gene="trg"
                     /locus_tag="ACHKW5_001069"
     CDS             46439..48079
                     /gene="trg"
                     /locus_tag="ACHKW5_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO7510503.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(48117..49040)
                     /gene="ydcI"
                     /locus_tag="ACHKW5_001070"
     CDS             complement(48117..49040)
                     /gene="ydcI"
                     /locus_tag="ACHKW5_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7510504.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            49257..50600
                     /gene="ydcJ"
                     /locus_tag="ACHKW5_001071"
     CDS             49257..50600
                     /gene="ydcJ"
                     /locus_tag="ACHKW5_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7510505.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            50825..52480
                     /gene="opgD"
                     /locus_tag="ACHKW5_001072"
                     /gene_synonym="mdoD"
     CDS             50825..52480
                     /gene="opgD"
                     /locus_tag="ACHKW5_001072"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO7510506.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            52620..52844
                     /gene="ydcH"
                     /locus_tag="ACHKW5_001073"
     CDS             52620..52844
                     /gene="ydcH"
                     /locus_tag="ACHKW5_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO7510507.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            52907..53443
                     /gene="rimL"
                     /locus_tag="ACHKW5_001074"
     CDS             52907..53443
                     /gene="rimL"
                     /locus_tag="ACHKW5_001074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO7510508.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(53438..54418)
                     /gene="ydcK"
                     /locus_tag="ACHKW5_001075"
     CDS             complement(53438..54418)
                     /gene="ydcK"
                     /locus_tag="ACHKW5_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO7510509.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            54542..55534
                     /gene="tehA"
                     /locus_tag="ACHKW5_001076"
     CDS             54542..55534
                     /gene="tehA"
                     /locus_tag="ACHKW5_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO7510510.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            55531..56124
                     /gene="tehB"
                     /locus_tag="ACHKW5_001077"
     CDS             55531..56124
                     /gene="tehB"
                     /locus_tag="ACHKW5_001077"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO7510511.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            56427..57095
                     /gene="ydcL"
                     /locus_tag="ACHKW5_001078"
     CDS             56427..57095
                     /gene="ydcL"
                     /locus_tag="ACHKW5_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO7510512.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(57194..57556)
                     /gene="tnpA"
                     /locus_tag="ACHKW5_001079"
                     /pseudo
     CDS             complement(57194..57556)
                     /gene="tnpA"
                     /locus_tag="ACHKW5_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT        14207 a        13717 c        15167 g        14588 t
ORIGIN      
        1 tatacgttat tctgcgcggg ttatatgcct ttattgtcac agattttatt ttctgttggg
       61 ccattgcatt gccactgatt ttccaacata taaaaagaca agcccgaaca gtcgtccggg
      121 ctttttttta gaattggata atccttatcc agagcattta atcggtgttg cttttttccg
      181 cttttccggc catttgtgcc aggaagtcat agcgtttttg caaatcagct gcagcatctt
      241 tccataactg ttctgccact tctggctgct gcgaattcag ccgacggaaa cgttgctcgt
      301 gaagtaacgt ttcttccagt gctactgacg gcgggcgtga atccagcgcc agcggcagtt
      361 tgccttcatc ggcacgacgc ggatcgaagc gatatagcgg ccagaagccg gtagctgtga
      421 gttggcgcat ctggtcgtgg ctgagtgcca gatcgtaacc atgctcttca catgggctat
      481 aagcgatgat cagcgatggc cccggatacg cttccgcttc ctgaatcgct ttcaccgtct
      541 ggttgagctg cgcgccgaga gaaatctgcg ccacataaac atgaccgtac atcatcatac
      601 tgacgccaag atctttacgc gctttacgtt tgccgtgctc gccaaattta gttactgcac
      661 ccagcggtgt cgctttcgac gcctgaccac cggtgttgga atagcattgc gtatccagca
      721 ccagaatgtt gacgttttcc gtcaaactca atacatgatc cagaccgcca aagccgatat
      781 cgtaagccca gccatcacca ccaatcagcc agattgattt ttccaccagt gcatctgcat
      841 cacgtagcag ttcatgtgct tcggcaacat cgttgagttg ctggcgtaaa gctgcgacct
      901 gttctcgacg aacctctggc gtagcgtctg atttcaacgc cgtcagtaat tccgccggga
      961 ttttatcggc aaattgatcc agcagacgca gcacgcggac acggtgttga tcgaccgtca
     1021 ggcggaaacc aaggccaaat tcggcattat cttcaaatag agagttcgcc catgccggcc
     1081 cacgaccgtt ggcatcggtg gtatacggtg tagagggcag gttaccgcca taaattgaag
     1141 aacagccggt ggcgttagcg atcaacatcc ggtcgccata gagctgagtc agtaatttaa
     1201 tatacggcgt ctcgccacaa ccggagcaag cacctgaata ttcaaacagc ggtgtaatca
     1261 gctgcgatgt acgaatatca atacgttcca gtttgctacg gtcgatttct ggcaggttga
     1321 ggaagaaatc gtaattgatt ttctcttctt cgacatgttc caggcgcgac atcatattga
     1381 tggctttaat ctctggattc tgacggtctt tcgccgggca aacttcgacg cacaggttac
     1441 aaccggtgca atcttccggt gccacctgca agacatattt ctgcccgcgc atatcacgcg
     1501 atttcacatc cagcgaatgc aggctggcag gggcgttttc catcgcttca ggcggcacca
     1561 cttttgcgcg aatagctgag tgtgggcaag cggcaacgca gtggttacat tgggtacaga
     1621 gttcctcttt ccagatgggg atctcttcgg cgatattgcg tttttcccag cgagtggtgc
     1681 ccatcggcca ggtgccgtct ggcggcagcg ccgaaacggg gagggcatca ccaagccctg
     1741 cgagcatcgc tgcggttacg gttttcacga aatcaggggc ggcatcggaa accactggcg
     1801 gtcgattggc gctgtgcgga tttaccggtt gcaacggaac ttcttctacg gattcacgcg
     1861 ccagcgccag agcctgccag ttgcgttcca ccagatcctg gcctttgcta ctgtaacttt
     1921 tggcaatcgc accctgcaat tctgcgaggg cgctatcgcc aggcagaatt tgcgtcagat
     1981 ggaaaaaagc catctgcatg acggtattaa tacgggccgc caggccacat tcgcgggcga
     2041 ttttcgccgc gttaatcaca tagaagcgcg cttttttctg atttagcacg gcctgaactt
     2101 cctgcggcaa gcgcgaccac acttcatctg cgctgtacgg cgtgttgagc aggaaaatgc
     2161 cgccaggttt taaacgctca gccatctgat atttatcgat aaactgcaac tggtggcagc
     2221 caacaaaatc agcctgggaa atgagataag cggaacgaat cggctgttcg ctcacgcgaa
     2281 ggtgagaaac cgtcaggccg cccgcctttt tggagtcgta aacaaaatag ccctgtgcgt
     2341 accacggcgt ggaattaccg ataatcttga tattgttttt ggtcgcggaa acgctgccat
     2401 cactaccaag gccataaaac aaggcttcca gtttcgccga gtttggcagg gtgttttccg
     2461 gcaacggcag tgacagattg gtcacatcat cgtaaatacc aaccgtaaag cgcgctttcg
     2521 gtttagccgc gttgagctcg gcaaataccg ccagtacaca gtccgggcca aattctttgg
     2581 atgaaagacc atagcgccca ccaatgacac ggggcagagt ttcgcgctcg ccattattaa
     2641 aggcttctgc cagtgcggtc attacatcca gatagagcgg ttctgcctgg gcaccgggtt
     2701 ctttggttct gtccagtacc gccacgctgc gtacggatcc cggcagagct tgcagcaagt
     2761 gtttggcgga gaagggacgg tacaggcgaa ctttcagcac gccaactttt tcgccacggg
     2821 ttagcaattc atcaaccact tcttcacagg tgccaatggc agagcccatc aggataatca
     2881 cccgttccgc ttgcggatgc ccgtaatatt caaacggctg atactgacga cctgtcgcgg
     2941 cagagaaatc attcatcgcc tgttcaacat ggtcatagac cgcgtcgtac catgggttgg
     3001 tggcttcgcg agactggaaa taagtgtcag gattggcgga cgtaccgcgg atcaccggat
     3061 gttccgggtt gagcgcccgg gcgcgatgag catcaatttc ggcctgcggc atgagatcaa
     3121 gaatagtgtc atcggccagc gggacaattt tattgatttc gtgggacgtg cggaaaccat
     3181 caaagaaatg aataaatggc acgcggctct tcagcgttgc gatgtgcgaa atgagagcaa
     3241 aatcttgcgc ttcctggacg ttagccgcac acaacatcgc gcaacccgtc tggcgcaccg
     3301 ccatgacgtc ggaatgatcg ccaaaaatag agagtgcatg tgtggcaacg gtacgtgccg
     3361 ctacatgcag gacaaacggt gtgagttcgc ctgccagttt gtacagcgtc gggatcatca
     3421 gcagcaaacc ctgcgatgac gtaaacgatg ttgaaagggc acccgtctgc aaagcgccat
     3481 gcacggtagc gatagcaccc gcttccgact gcatttcaac cacgcgtggt gtgtctcccc
     3541 aaacgttctt caagccgttt ccggcccagg catcagcctg ttctgccatc gtggaactgg
     3601 gggtaatagg gtagatggcg ataacttcac tggtgcgaaa cgccaccgaa gcaaccgcgc
     3661 cattaccgtc aatagtaatc atatgacacc cttacattgc gcaaatgagg ggcgcacgaa
     3721 attgctgcgc gcccagtagt aatccttcaa ttttagcaaa tggctttctt ctgcattttc
     3781 gcttttgtgt cccccacatc agcgtaatga atgttttgat caaacagagg gcaaaaaaat
     3841 agccataaaa aagtaaaaat gcggggcaac gcacaggaca gctctcgacg ccgccctctg
     3901 tgctgcctat tatttatggc ggtgtcgttt ggcttgaaga tcagaggaag aagagatgcg
     3961 agcagcgttt tgggtagggt gtgccgcttt attgttgtcg gcgtgtagta gtgaacctgt
     4021 tcagcaggcg actgcggcgc acgtagcgcc aggtttaaaa gcgtcgatgt ccagtagtgg
     4081 agaagcaaat tgtgcaatga tcggcggttc gctttctgtt gcccgtcaac tggatggtac
     4141 ggcgattggg atgtgtgcat tacccaacgg caaacgctgt agcgaacagt cacttgccgc
     4201 cgggagctgt ggcagctatt aattcattaa atccgccagc ttataagtta atgtctgttt
     4261 cgcggtcgcc agcgttaact ggttcgcggt cagatccact tgtgcacctt ctttcagcat
     4321 ttcgctaatg gtgttatcga gttcattaag ctgcgggtta gcgcacatca tacgggtcat
     4381 tgccagccct ttggctgtca gttcaccatt agacagtttg ccttcaccgc taaagcggtt
     4441 acacatgctg ccggaaatca tcattttttc accaaagctg atttctggcg gatttttatc
     4501 gctggtcacg ggcttaccgt ttacgctttc cagcacaaag cgatgatgct gtagctgttc
     4561 tggcgtaaca gcaattttgt cattacttac acatcccgcc atcagcaggc ttagcgcaac
     4621 aaacgcggct actttcttca ttgtggttct caattacagt ttctgactca ggactatttt
     4681 aagaatagag aatgaaaggt cattggggat tatctgaatc agctcccctg gattgcaggg
     4741 gagcggcaag attaaaccag ttcgttcggg caggtttcac ctttttccag attgcttaag
     4801 ttttgcagcg tagtctgaga aatactggtc agagcttctg ctgtcaggaa tgcctggtgc
     4861 ccggtaaaca gcacgttgtg gcaggcagac aggcgacgga atacgtcatc ctggatcacg
     4921 tcgttggatt tatcttcaaa gaacagatcg cgttcgttct catacacgtc catacccaac
     4981 gaaccaattt tctgattttt cagcgcttca attgctgcct gagaatcaat caatgcaccg
     5041 cgactggtat tgacgatcat cacgccattt ttcatctgat cgaaggcggc ttcgttcaac
     5101 agatggtagt tttccggtgt cagcgggcag tgcagagaga taacgtctga ttcagagaac
     5161 agggttggca gatcgacata ctccacaccg agttccagcg ccgctgcact tggatacgga
     5221 tcgaacgcca gcagacgcat accaaaacct ttcagaatgc gcagcatcgc cacaccgatt
     5281 ttaccggtac cgataacgcc tgccgttttg ccatacatag taaagccggt cagaccttcc
     5341 agagagaagt tagcgtcacg ggtacgctga tacgcgcggt gaatacggcg gttcagcgtc
     5401 atcatcatac cgatggcgtg ttcagcaacg gcctctggat cataggctgg aacacggact
     5461 actttcagcc ccagttcttt tgccgcgtca aggtcgacgt tattgaaacc ggcacagcgc
     5521 agggcgatat atttaacgcc gtgctttttc agctcttcca gcaccgggcg gctgccgtca
     5581 tcgtttacga aaatacatac cgcttcgcag ccattggcag ttttagcggt tttttccgtc
     5641 agcagaaagt caaaaaattc cagctcaaag ccaaaggact cgttcacctg ttgcaggtac
     5701 ttcttgtcgt actgttttgt gctataaacg gcgagtttca taagactttc tccagtgatg
     5761 ttgaatcaca tttaagctac taaaaatatt ttacaaaatt tcaaatttaa ttgaaagcta
     5821 tggcgatatt gaaaaattca tcaacaacta tgcttagtgt aggcgcaacc ttcaactgaa
     5881 cggttaaaca tgccacaata cccgtattga atgcttaatt tttcgctaaa tcaggatatt
     5941 aactacccat gctgggtaaa tataaagccg ttctcgcgct gttattactg attattcttg
     6001 tgccgttgac gctgttgatg acgctcgggc tgtgggttcc cacgctggcg ggcatctggc
     6061 taccgctcgg gacacgtatt gcattagatg aaagcccacg cattacgcgt aaaggtttaa
     6121 tcattcccga tctccgttat ctggtgggag attgtcagct tgcgcatatc accaacgcca
     6181 gcctttcaca tcccagccgc tggttattga acgtcggcac ggtagaactt gattctgctt
     6241 gtctggcgaa attgccgcag acggagcaat cgccagctgc tccaaaaacc ctcgcgcagt
     6301 ggcagtccat gctgcctaac acctggatca atatcgataa actgattttt tctccctggc
     6361 aggaatggca gggaaaactc tctctcgcat taacctctga tatccagcaa ctgcgttatc
     6421 agggcgaaaa agttaaattt caaggccagc tgaaagggca acaacttaca gtcagcgaac
     6481 tggatgtcgt cgcgtttgaa aatcagccgc cggttaaact ggtgggggaa tttactatgc
     6541 cgctcgtgcc ggatggactt cctgtaagtg ggcatgctac tgcgacgtta aacttgccgc
     6601 aggaaccgtc actggtggat gcagagctgg actggcagga aaatagcggg caattgattg
     6661 tgctggcacg ggataacggc gatccgttgc tcgatttgcc gtggcaaatt actcgtcaac
     6721 aattgaccgt aagcgatggt cgctggagtt ggccgtatgc aggttttcct ttgagtggcc
     6781 gactgggtgt caaagtcgac aactggcagg cagggcttga gaacgctctg atcagcggac
     6841 gactgagtgt gctgacccag gggcaagcgg gtaaaggcaa cgcggtgcta aattttggcc
     6901 caggaaaatt aagcatggat aacagtcagc tgcctctgca gctgaccggt gaagcgaaac
     6961 aggcggacct cattttatat gcccgtttac ctgcgcagct aagtggaagt ctgtctgacc
     7021 caacgctgac ctttgagcca ggcgcgttac ttcgttcaaa gggaagagtc atcgattcgc
     7081 tggacatcga tgaaatccgc tggcctttag cgggtgtaaa agtcacccaa cgtggtgttg
     7141 acggacgttt gcaggccatt ttgcaggcgc atgaaaatga actgggcgat ttcgtgctgc
     7201 atatggatgg gctggcgaat gattttctcc ctgacgcggg ccgctggcag tggcgctact
     7261 ggggaaaagg cagttttaca ccgatgaatg ccacctggga tgtcgcagga aaaggtgaat
     7321 ggcatgacag cacgattacg ctgaccgacc tctccaccgg tttcgaccag ttacaatacg
     7381 gtacgatgac ggtagaaaag ccgcgattaa ttctcgacaa gcccgtcgtc tgggtacgtg
     7441 acgcacagca tccctccttc agcggcgcgc tgtcactgga cgccgggcaa acgctgttca
     7501 ctggcggcag tgtgttaccg ccatcaacct taaaatttag cgtcgatggg cgcgatccga
     7561 cgtatttcct ctttaaaggc gatttacatg ctggtgagat tggcccggtt cgggtaaatg
     7621 gtcgctggga cggtattcgt ctgcgcggta acgcctggtg gccaaaacaa tcactgactg
     7681 tattccagcc gctggtgcca cccgactgga agatgaactt acgcgatggt gaactgtatg
     7741 ctcaggttgc attttctgct gcgccggaac aaggattccg cgcgggaggg cacggcgtgt
     7801 tgaaaggcgg tagtgcctgg atgccagata atcaggttaa cggtgtcgat tttgtcctgc
     7861 ctttccgttt tgccgatgga gcctggcatc tggggactcg cggccccgtt acgttgcgaa
     7921 ttgccgaagt gattaatctg gtgacagcga aaaatattac ggctgatttg caagggcgtt
     7981 atccgtggac tgaagaagaa cccttgctgt tgactgatgt tagcgtcgat gtgttaggcg
     8041 gtaacgtact gatgaaacaa ttacgtatgc cgcaacatga cccggcgctg ttgcggctga
     8101 ataatctctc atccagcgaa ctggttagcg ccgtcaatcc gaaacaattc gccatgtccg
     8161 gggcatttag tggtgcattg ccattatggc taaacaacga aaaatggata gtgaaagatg
     8221 gttggctggc aaatagcggg ccaatgacct tgcggctgga taaagacacc gcagatgcgg
     8281 tggtgaaaga caatatgact gcgggttcag caattaactg gttgcgctat atggaaatta
     8341 gccggtcatc gacaaaaatt aacttagata atctcggttt attaaccatg caggccaaca
     8401 ttacaggtac cagtcgcgtt gatggtaaaa gcggtacggt aaaccttaat taccatcatg
     8461 aagagaatat ttttacgctg tggcgcagtt tacgctttgg cgataatctc caggcatggt
     8521 tggagcagaa cgcacgtctg ccgggaaatg actgtccgca aggaaaagag tgtgaggaaa
     8581 aacaatgaaa attttactgg ctgcgttgac gtcatctttt atgctggttg gctgtacgcc
     8641 tcgcattgaa gtcgctgcac ctaaggaacc gatcactatc aatatgaacg ttaaaattga
     8701 gcatgagatc atcatcaagg cagacaaaga tgtcgaagag ctgcttgaaa ctcgtagcga
     8761 tcttttctga ggcgatgatg aagaaaacat tacttctttg tgcgtttctt gttgggctgg
     8821 taagcagcaa tataatggca ttgactctgg atgaagccag aactcagggg cgggtaggtg
     8881 aaacatttta tggttatctg gttgcgctga aaacggatgc tgaaacagag aaattagtaa
     8941 ccgacattaa tgccgaacgt aaagcgagtt accaacaact ggcaaagcaa aataatgtgt
     9001 cggtagatga tatcgcgaaa ctcgccgggc aaaagctcgt agctcgggcc aaaccggggg
     9061 aatatgtaca agggataaac ggtaaatggg tgcgaaaatt ttaatagcaa aaccgcaata
     9121 tcgggataat gctacaggag aaagcgattg atctattttc ctgaaacaag gtgaatatta
     9181 aaaaactcct gttaaattgc cttttgccct gaaaaatgca tagtcaaata tctgttttaa
     9241 ctaattggcg ttgcagtaca tgcaacgcga attagttagc ggaatttacg ccgatactcg
     9301 cctggcgtca tcccaaagcg ttgcttaaat actgttgaaa aatgactctg gtcggaaaat
     9361 ccccaatgaa aaccaatgcc tgccagtttt tcatcatctg cggcatggcg aatcgcatca
     9421 gcacaaaaat cgagacgacg gttacgaata tattgcgcga ccaccaaacc tttatcggca
     9481 aacattcggt acaaactacg tactgacata cctgtctctc cggctatcca ctccgggcgt
     9541 aatatctctt cgcgaatatt atcgtctatc agcgtaacca ctttttgaaa ctgacgttca
     9601 cggcgaggtt gaacagattc ccgctgatga agtaccgggc gcagcagaca caccatcgcc
     9661 tgtagcgcag cttcactttc tgtttcagaa agtgccgggt tattcatgct ctcctgtaac
     9721 aggcgatgac tgagttgcac catgggtaag tcagcgtcca gtctttctgc gcagacaggt
     9781 ttttgatggg gaaaatattg ttccagcaga gtgcgtggta aaagtaatga aatctgttta
     9841 gaagactcct gccagtaaag cgaacagggg cgtgaggcat cgagtaacgt aatatcgcca
     9901 gcgccaatct gcacctgacg ctcatcctgc tccattattg cctgaccact aagttgaaaa
     9961 acggtgtaaa accaggcatc gtcgctgcct tttacctcct gccaggtgcg gtataaattc
    10021 actccgttgg ttgtcacggt gctcagcttt agtcctttgg caaaatgcgt ttccagtaca
    10081 cccgtgtaac gctcagtcag caggcgtccg gtaaaatttc cgcatacctg attgatttgg
    10141 gaaagccatt gctgaaactc attatccact gcggggttca tggcacgttt tcgctctgtg
    10201 aaatgtattt ttattgttgc atttgtgttg caataaacga agctaatgag cctgactata
    10261 ggaaataagt cttgtcaggc atagagacat aagcggttat tgtcacgatt tgcggagctt
    10321 gtcacagctg acaaagcgaa tgtcacagcg aaaaaagtga cttttcttgt cgctgcgtac
    10381 actgaaatca cactgggtaa ataataagga aaagtgatga cagagccgca tgtagcagta
    10441 ttaagccagg tccaacagtt tctcgatcgt caacacggtc tttatattga tggtcgtcca
    10501 ggccccgcac aaagtgaaaa acgattggcg atctttgatc cggccaccgg gcaagaaatt
    10561 gcgtctactg ctgatgccaa cgaagcggat gtagataacg cagtcatgtc tgcctggcgg
    10621 gcctttgtct cgcgtcgctg ggccgggcga ttacccgcag agcgtgaacg tattctgcta
    10681 cgttttgccg atctggtgga acagcacagt gaggagctgg cgcaactgga aaccctggag
    10741 caaggcaagt caattgccat ttcccgtgct tttgaagtgg gctgtacgct gaactggatg
    10801 cgttataccg ccgggttaac gaccaaaatc gcgggtaaaa cgctggactt gtcgattccc
    10861 ttaccccagg gggcgcgtta tcaggcctgg acgcgtaaag agccggttgg cgtagtggcg
    10921 ggaattgtgc catggaactt tccgttgatg attggtatgt ggaaggtgat gccagcactg
    10981 gcagcaggct gttcaatcgt gattaagcct tcggaaacca cgccactgac gatgttgcgc
    11041 gtggcggaac tggccagcga ggctggtatc cctgatggcg tttttaatgt ggtcaccggg
    11101 tcaggtgctg tatgcggcgc ggccctgacg tcacatcctc atgttgcgaa aatcagtttt
    11161 accggttcaa ccgcgacggg aaaaggtatt gccagaactg ctgctgatca cttaacgcgt
    11221 gtaacgctgg aactgggcgg taaaaacccg gcaattgtat taaaagatgc tgatccgcaa
    11281 tgggttattg aaggcttgat gaccggaagc ttcctgaatc aagggcaagt atgcgccgcc
    11341 agttcgcgaa tttatattga agcgccgttg tttgacacgc tggttagtgg atttgagcag
    11401 gcggtaaaat cgttgcaagt gggaccgggg atgtcacctg ttgcacagat taaccctttg
    11461 gtttctcgtg cgcactgcga caaagtgtgt tcattcctcg acgatgcgca ggcacagcaa
    11521 gcagagctga ttcgcgggtc gaatggacca gccggagagg ggtattatgt tgcgccaacg
    11581 ctggtggtaa atcccgatgc taaattgcgc ttaactcgtg aagaggtgtt tggtccggtg
    11641 gtaaacctgg tgcgagtagc ggatggagaa gaggcgttac aactggcaaa cgacacggaa
    11701 tatggcttaa ctgccagtgt ctggacgcaa aatctctccc aggctctgga atatagcgat
    11761 cgcttacagg cagggacggt gtgggtaaac agccatacct taattgacgc taacttaccg
    11821 tttggtggga tgaagcagtc aggaacgggc cgtgattttg gccccgactg gctggacggt
    11881 tggtgtgaaa ctaagtcggt gtgtgtacgg tattaatctg gttcgctcat aagtaaaaaa
    11941 cggcacctgg tgccgttttt ttgtctgaaa caatcactta tctttcttca gcgcccctag
    12001 cgttggcgtt tcgtcaaaga agttccatgg tttaagcaga gtatgtaccc attcggtcgg
    12061 cataatcggc cactcttcgg cgcgggccac atgtgtggtg ccggtggtca tccagacaac
    12121 ggcgtcggtg ttgtccagcg actcgttatc tttactgtat tgtccaagac cggtgtcatg
    12181 agtagaacgg ttcggatatt tgccttccgg gaaacgctcg ccaggatgat aacgcgttac
    12241 ccaaagctgc ttgtccataa agcttaaacg atgatagatc cactcgtccg gcgcgaactg
    12301 ggcacctttt gctaccgggt gagtaccacc tgcataagga ataatttgat aggaaactgg
    12361 attgcccatg cggttctctt tgttcgggtt actcaacaga cgaatcgtgc ccggatcaaa
    12421 cttctgcgcg gcatcctgtt cattgccgat gttgtactga ttaacttgca tggtactggt
    12481 gcgtgggcca ccggcagtat tcggttttac cactgggtcc atcgccacca ggctgttatt
    12541 ctcgccatct acatccagat cgaggcggaa attataaata tgttggtgtg tagtacccac
    12601 gatattgtga tcgataagcg tgccgtagcg cgtgtcatct ttcgccgtct catcgtgcat
    12661 ggttttcgct ttaacacctt tcaccgcttc gatgcccgta gcaccggcat caatgccaat
    12721 agtgccgttt tcatggaaga tccagtcaaa aatgtagtca tagttaccca ctgtactgat
    12781 ccagcgcacc actaactccc ggcgttcggt actgacgttg ggctggccca tttcctgatg
    12841 cttatactcc ggcccggcat aacgttcaaa taccgcgata gcgcgaggga tctccatcgg
    12901 cacgccagtg tagtcggcga tggtttcatt aaggagcact gcgttagacg gggcatcttt
    12961 accacgagca attggtgagg ttagcgtgcc cataccgtag tcaccagagt ccagatacgc
    13021 tttaaagtac cagccaatat caggatcacc gtaaggcaca atcatgccgc cgagagaacc
    13081 ttcgtacatg actttgcgtt tggtgccatt gtcgttataa gtcacggtgg agatcatcgg
    13141 cccgacgcga gagttcatgc tgaggtgaaa atcccagttc cgccagtgaa tcatatcgcc
    13201 agtaatggtg taatttttac cttcaggctc aatgatttgc ataggcttaa ctgccggagc
    13261 aacgcggtca cggctatcaa atgggcgtgc ggtcattggc accggaacta ccggaccttc
    13321 ttcaatctta acgatttttt tctgttctaa atcaacgacc gccaccaggt tttcgatggg
    13381 atgtgcccag tagttgccat caccgacatc aagatagctg atgactttga gcaaccgggc
    13441 atcttgtttc aggccatctt taccatcgaa ataacctacg gtcagcggcg tggtaatcac
    13501 ttttttcgca tcagtaatac cgcgtttctt cacggcagcg gcaaattctt cactgttgtt
    13561 aataatgttc tgcacactgg cgaaatcatc cagcaacacc ataccgtggg cgtctttagt
    13621 gggttgccag gagagcagtt tgttgttttg cagatccacc accgcttcga tgatatgttt
    13681 gccgtcgagc ataatgacgt cggctttgcg cggctggtca accggtttgt tttccagcgc
    13741 aaacgcccag acagcttctt tatctggcgg tagcagggag atctcagtaa aacgggtatt
    13801 gggtttgaag tccgcggaag ctttaacaat ttcaacggcc tgtttaattt cgtccgcagt
    13861 tagcgcatta agtgggtgag ggcgcttttc tacctgaaag gtttgatcca gcccggactg
    13921 gaaaacatcg ttaataaagg tgtcagaaac ccaggcttta ttgtctttca tcactaccgg
    13981 tacttgcagt gccagaggct gaccattaac aattgctgtt tgcgcaccag gcttcacttt
    14041 cacgtacgcg ccatctttaa tcagggtaaa gagctgggcg tagtcgtccc actgcacatc
    14101 ggcaccaaat tctttaagcg ttttatccat tggcaccata tgcgcttcac caccgtgggc
    14161 aaataccggc gcttgccagg cgaaacttaa ggcgactgcc aacgccaggg ttgttttacg
    14221 ggcagaatac agagaggggc ttcccattat taacctcgtc agatgttgtg ttcttgttag
    14281 caaccgcgct ctgtgggcgg tttagtcagg ttcacattat cagtactgat gcaaagggga
    14341 ttgcctgcac ctgccaggtt gtttggcagg tgtgccagct tttcatacag tggatgccct
    14401 gaaaatagat gtacacatca tgcataatgt gacaacgtca caaaatttag tgaaataaaa
    14461 gggcaactat tcgccgttgc ccttcattca ccgattaatc gacaaaatca ccgtgctgcc
    14521 tggccaccag cgtcagaatt gaatacagcg ccaccggggt ttgatgctga ttgaatacct
    14581 ctaccgccca ttccaccaca cctgttggtt tttcttctgc gctacgctgt tttttcagcg
    14641 tcttgcgctt acaggtgaga cgcacctgga tggtatcgcc tggctttacg ggttcgataa
    14701 aacgcaagct ttccagcccg tagttagcaa tgaccggacc aacaccggca tcgacaaaca
    14761 gacccgcagc cgcagaaagc acaaaatacc catgcaccac ccgctcaccg aaaatagatt
    14821 cggcagcagc aatcttatcc atatgtgcat agaaatgatc gccgctgagg caagcaaagt
    14881 taacaatatc ggcctctgtc attgtgcggc ggggagtcaa caggctgtcg cctggttgta
    14941 gctcctcaaa atatttgcgg aacggatgaa tacgatcttc ttcgactttc gcaccgcgca
    15001 cccactgttt actgatagcg gcaagcatcg tcggactacc ctgaacagcg gttcgctgca
    15061 tgtaatgttt caccgctcgt aaaccgccta attcttcacc gcctcctgcg cgaccaggcc
    15121 caccatgtac cagttgtggc agtggggagc catgcccggt ggattctttt gccgattctt
    15181 cattgaggat ctgaattcgc ccatgcgtac gtgccgcgtc ggcaataaac tgacgcgcaa
    15241 tttgcggatc agccgtcacc agcgttcccg caaggctacc gccgcctgca caagccagtt
    15301 gcagagcatg ttgctggttt tgtgctggca ttagcgttgc gacagggcca aaggcttctg
    15361 ttgcatgtac cgccggtgtt tcatccggct gcggacagta caataaggtt ggcgggaaga
    15421 aggcacccgc cgcagataaa tccgcctgac caccgaggcg aatctcgcat cctgcagcca
    15481 gcaatgtgtt cactttttcc tgcacatcag cacgctgttc agcatttacc agtgcgccca
    15541 ttttcacgcc ttcttgtgca ggatcaccga ccacgacttt ctgtaatcgc gcaaccagag
    15601 catcactgac agcattaacc aatgtctgcg gcacaataat ccgccggatt gccgtacatt
    15661 tttgcccggc ttttgtggtc atctcacgca caacttcacg aataaacagc gcaaactccg
    15721 gttgatccgg ggtgatatct tcgcccagta cgcagcaatt cagggaatca gcttccatgg
    15781 tgaaggggat agatttggct acgatatttg gctgaactcg tagcatctgt ccggtcgtcg
    15841 cggaccccgt gaaagtcacc acatcctggc tgtccagatg atccaaaagg tcgcccgcac
    15901 taccgcagat cagactaatt gcgccttcgg gaacaagacc actatcgaca attgatttca
    15961 ccatcgcctg agtcagttgg gccgtcgcgg tagctggttt gatgatggct ggcattccgc
    16021 ccagccacgt tggtgccagc ttttccagca ttccccagca ggggaagtta aaggcgttaa
    16081 tatgcactgc cacgcctgac tttgaggtca gtacatggcg cgcggcaaat ccaccttctt
    16141 tcgataaggg gagcaattca tcttccggcc acagcgtatc gtcaggcagc tcccggctac
    16201 cgaggctggc gtaagtaaat aacgtcccaa tgccaccttc aatatcaacc caactgtctg
    16261 cccgcgttgc gcctgtttgc gcagaaagag catagaaacg ctctttttca ctcagcagat
    16321 gtttagcgac cgctttaagc atcgccgcac gttcgataaa agtcatagcg cgaagggctg
    16381 gggcaccttt ttcaatggca aacaggcggg cagccgccat atcaagacct tcactggtca
    16441 cttcccataa cgcctcgccg ctaatagcgt ggtgaatcaa acggctacgg ccccggccag
    16501 actgccaggt accggataag aaactggcta actgctgcat cgctactctc cagatgtttc
    16561 acatttctgt tgctaatagt tgaatcgcga atcataaaaa gcaagggatc ttttaatgaa
    16621 atgttaacta tgcgatctgt atagcaactg cggaaaacat taatgcactg ataaataatg
    16681 atttataaaa agagggtgcg aaatccgtca cagttcaagc atacaaaatt tgtgatttta
    16741 gttaaccatt gtgtaacttt cataaaacaa tgtgattcgt gtttttaatt aattcacgaa
    16801 aactggaatc gtaaaggtga tgacgtgacc caagaagaac gctttgagca acggatagcc
    16861 caggaaacgg ctatcgagcc acaggactgg atgcccgatg cttaccgaaa gacattgatc
    16921 cgccagattg ggcagcatgc gcactccgag attgttggta tgttgcctga aggtaactgg
    16981 atcactcgcg caccaacctt gcggcgtaaa gccattctgt tagccaaagt gcaggatgaa
    17041 gccggtcatg gtctgtatct ctatagcgcc gccgaaacac tgggctgtgc cagagaagac
    17101 atctaccaaa aaatgctcga cggacggatg aaatattcct ccatctttaa ttatccgaca
    17161 ttgagttggg ctgatatcgg cgttatcggc tggctggtgg atggcgcagc gatcgtcaat
    17221 caggtggcgt tatgccgaac ttcttatggc ccgtatgcca gagcaatggt gaaaatctgc
    17281 aaagaagaga gttttcacca gcgtcagggg tttgaagcct gcatggcact ggcgcagggc
    17341 agcgaagctc aaaagcagat gttgcaagac gccattaacc gtttctggtg gccagcctta
    17401 atgatgttcg ggccaaacga tgataactcg ccaaacagcg ccagaagtct cgcctggaaa
    17461 atcaaacgtt tcaccaacga cgaactccgc cagcgtttcg tggacaacac cgttccacag
    17521 gttgaaatgc tcggtatgac cgttcctgac ccggatctgc attttgacac tgaaagcggt
    17581 cactaccgct ttggtgagat cgactggcag gagtttaacg aagtaattaa cggtcgcgga
    17641 atttgtaatc aggagcggct tgacgccaaa cgtaaagcct gggaagaagg tacctgggta
    17701 cgggaagcag cgctggccca tgcacaaaaa caacatgccc gtaaggtcgc ataaggagat
    17761 tcaaaatgag taatgtttac tggccgttat acgaagtttt cgtgcgtggc aaacagggct
    17821 tatcacaccg ccatgttggc agtttacatg ctgccgatga gcggatggca ctggaaaatg
    17881 cccgtgatgc ttacacccgt cgtagcgaag gatgttcaat ttgggtggtg aaggcgagtg
    17941 aaattgttgc ctcgcaaccg gaagaacgcg gtgaattttt tgatccggct gaaagcaagg
    18001 tctatcgcca tccaacgttt tacaccatcc ctgatggcat tgagcacatg tgaggtcgga
    18061 aatgaatcag ttaacggctt acaccttgcg cctgggcgat aactgcctgg tgctctccca
    18121 gcggttgggt gaatggtgcg gtcatgcacc ggaactggaa atcgatctcg cactggcaaa
    18181 tattggactc gatttattag gccaggcacg caacttctta tcgtatgccg ctgaattagc
    18241 gggagaaggc gatgaagata ccctggcctt cacccgagac gagcgccagt tcagcaactt
    18301 attgttggtt gaacagccaa acggcaattt tgccgacacc attgcacgcc agtatttcat
    18361 cgatgcatgg catgtggcgc tctttacccg tctgatggag agccgtgatc cgcaactggc
    18421 ggcgatttct gccaaagcaa ttaaagaagc gcgttatcac ctgcgtttta gtcgtggctg
    18481 gctggagcga ctgggcaatg gtactgacgt atcagggcaa aagatgcagc aggccatcaa
    18541 caagttgtgg cgttttaccg ccgaactgtt cgatgccgac gagattgata ttgcactgag
    18601 tgaagagggt attgcggttg atccacgcac tttacgcgca gcgtgggaag ccgaagtttt
    18661 tgccgggatc aacgaagcca cattgaacgt accgcaagag caggcgtatc gcactggcgg
    18721 taaaaaagga ctgcataccg aacaccttgg acccatgctg gcagaaatgc agtatctcca
    18781 gcgtgtcttg cccggtcagc aatggtaaca gaggagatgg gtatgcaacg tctggccacc
    18841 attgcaccgc cacaggttca tgagatatgg gcgctactca gccagatccc ggacccggag
    18901 atcccggtgc tgaccattac cgatttaggc atggtgcgta atgtgacaca gatgggagaa
    18961 ggatgggtga tcggctttac gccgacttat tccggttgtc cggcaacgga acatttgatt
    19021 ggcgcgatac gtgaggcaat gacaacccac gggtttaccc ccgttcaggt tgtgctgcaa
    19081 ctcgacccgg catggaccac cgactggatg acccccgatg cccgtgagcg tctgcgacag
    19141 tatggcatta gcccgcccgc cggacacagt tgccatgccc atttgccgcc agaagtacgt
    19201 tgcccgcgct gcgccagcgt ccataccaca cttatcagtg aatttggttc cacggcctgc
    19261 aaagcattgt atcgctgcga tagttgccgc gaacctttcg attatttcaa atgtatttga
    19321 ggatgccatg acaacgtttc attccttaac ggtggcaaaa gtggagccgg aaacccgtga
    19381 tgcggtgacc attacctttg cggtgcccca gcctttgcag gaggcgtatc gctttcgccc
    19441 cggtcaacat ttgaccttaa aagccagctt tgatggtgaa gaattacgcc gttgttactc
    19501 catttgccgc agctatctgc ctggcgaaat tagtgtggcg gtgaaagcca ttgaaggcgg
    19561 acgtttctcc cgctatgccc gcgaacacat ccgccagggt atgacgctgg aggtcatggt
    19621 gccgcagggg catttcggct atcagccaca ggccgaacgc cagggccgct atctggctat
    19681 tgcagcagga tcaggtatta cgccaatgct ggcgattatc gccaccactt tacaaaccga
    19741 gcctgaaagt cagttcaccc tgatctacgg taaccgtacc agccagagca tgatgtttcg
    19801 ccaggcactg gcagacctga aagacaaata tcctcagcgt ttacagttgt tgtgcatttt
    19861 cagtcaggaa accctcgaca gcgatctgct tcacgggcgt attgacggtg aaaaattaca
    19921 gtcacttggg gcctcgctca ttaattttcg tctttatgat gaggcattta tttgtggtcc
    19981 ggcggcgatg atggatgaga cggaagccgc cttaaaagca ctgggaatgc cagataaaac
    20041 cattcatctg gagcggttta atacgcctgg cacgcgcgtc aaacgtagcg ttaacgtgca
    20101 aagtgacgga caaaaagtga ctgtacgtca ggatgggcgg gatcgggaaa tcgtgcttaa
    20161 tgccgacgat gaaagcattc tcgatgcggc attgcgccag ggggctgatc tgccctatgc
    20221 ctgcaaaggc ggcgtctgtg cgacctgcaa atgcaaagtg ctgcgtggca aagtggcgat
    20281 ggaaaccaat tacagtctgg aaccggatga actggccgca ggctatgtgt tgagttgcca
    20341 ggcactgccg ctgaccagcg atgtggtggt tgactttgac gcgaagggga tggcatgagc
    20401 gaactgatcg tcagccgtca gcaacaagta ttgttgctga cccttaaccg tcccgccgca
    20461 cgtaatgcgc taaataatgc ccttctgacg caactggtaa atgaactgga agctgcggct
    20521 accgatagca gcatttcggt ctgtgtgatt accggtaatg cacgcttttt tgccgctggg
    20581 gccgatctca acgaaatggc agaaaaagat ctcgcggcca ccttaaacga tacacgcccg
    20641 cagctatggg cgcgattgca ggccttcaac aaacctctca tcgcagccgt caacggttac
    20701 gcgcttggtg cgggttgcga actggcattg ttgtgcgatg tggtggttgc cggagagaac
    20761 gcgcgttttg gtttgccgga aatcactctc ggcatcatgc caggcgcagg aggaacgcaa
    20821 cgtttaatcc gtagtgtcgg taaatcgtta gccagcaaaa tggtgctgag cggagaaagt
    20881 atcaccgctc agcaagcaca gcaggccggg ctggttagcg acgtcttccc cagcgattta
    20941 accctcgaat acgccttaca gctggcgtcg aaaatggcac gtcactcgcc gctggcctta
    21001 caagcggcaa agcaagcgct gcgccagtcg caggaagtgg ctttgcaagc cggacttgcc
    21061 caggagcgac agttattcac cttgctggcg gcaacagaag atcgtcatga aggcatctcc
    21121 gctttcttac aaaaacgcac gcccgacttt aaaggacgct aatgatggaa ttcatcctca
    21181 gtcatgtaga aaagggcgtg atgacactaa cgctcaaccg cccggaacgc ctgaacagtt
    21241 ttaatgatga gatgcacgca caactggcag agtgcctgaa acaggtcgag cgcgacgaca
    21301 ctatccgttg cctgttactt acaggtgccg ggcgcggatt ttgtgctggt caggatctta
    21361 acgatcgtaa cgttgatccc acaggccccg caccggattt agggatgtca gttgaacgtt
    21421 tctataaccc actggtacgt cgcctggcaa aactgccaaa accggtgatc tgtgcagtca
    21481 atggcgtggc ggcaggggct ggcgcaacac tggcactggg atgcgacatc gttattgctg
    21541 cccgttcagc aaaattcgtc atggcgttta gtaagttagg tttagtaccc gattgcggtg
    21601 gaacctggtt actgccacgc gttgctgggc gagcgcgcgc catggggctg gcactgctgg
    21661 ggaatcaact gagtgctgaa caggcgcacg aatgggggat gatctggcag gttgttgatg
    21721 atgaaacgct ggcagatacc gcgcaacagc tggcacggca tctggcgaca caaccgacat
    21781 ttggtcttgg acttatcaag caagcgataa atagcgctga aaccaatact ctcgatacgc
    21841 aactggatct ggaatgcgac tatcagcgac ttgccggacg gagcgcagat tatcgtgaag
    21901 gtgtcagtgc gttcctggct aaacgctcac cgcagttcac ggggaaatag catgatgata
    21961 aatgtgcaaa ctgtggcagt gattgggagc ggcaccatgg gggctggcat tgctgaagtt
    22021 gccgccagtc atggacatca ggttttactg tatgacattt ctgctgaagc gctgacccgc
    22081 gcaatcgacg ggatacacgc gcggctaaat tcacgcgtga cgcggggaaa actgactgct
    22141 gaaacctgtg aacgcacatt gaaacgcctg atcccggtga ccgatattca cgcgctggca
    22201 gctgcggacc tggtcattga agcggcgtct gaacgtctgg aagtcaaaaa agcgctcttt
    22261 gcacagctgg cggaagtttg cccgccacaa acgctattga ccactaacac ttcgtcaatc
    22321 tctataaccg cgattgctgc ggaggtaaaa aatcctgaac gtgttgcggg gctgcatttt
    22381 tttaacccgg caccggtgat gaagttggtg gaggtggtca gtgggctggc aacggcagcg
    22441 gaagttgttg agcagttgtg tgaactaacg ttgagttggg gtaagcagcc tgtgcgctgt
    22501 cattcgaccc ctggatttat cgttaaccgt gtggcgcgtc cttattattc cgaggcctgg
    22561 cgggcactgg aagagcaggt tgctgcacca gaagtgattg acgctgcact tcgcgatggc
    22621 gctggtttcc cgatggggcc gctggaatta accgatctga ttggtcagga cgtcaatttt
    22681 gctgtcacct gttcggtgtt taacgctttc tggcaggagc gtcgtttttt accttcgctg
    22741 gtgcaacagg aactggtgat tggtggacgg ttgggcaaga aaagtgggct gggcgtgtac
    22801 gactggcgcg cggaacgtga ggcagttgtt ggcctggaag cggtaagcga cagttttagc
    22861 ccaatgaaag tagaaaagaa aagtgacggt gtcacggaaa ttgacgatgt tttattgatt
    22921 gagacacaag gcgagacggc acaggcgctg gcaatacgac tggcacgccc ggtggtagtg
    22981 gtcgacaaaa tggcgggcaa ggtggtgacc attgctgcgg cagcggtgaa cccggactca
    23041 gcgacccgca aggccattta ttacctgcaa cagcagggca aaacagtgct gcaaattgca
    23101 gattacccag gaatgctgat ttggcgaacg gtagcaatga tcatcaatga agcccttgat
    23161 gcgctgcaaa aaggcgtggc ctctgaacag gatatcgata ccgccatgcg tcttggggtg
    23221 aattatccat atggcccgct tgcctgggga gcgcaacttg gctggcagcg aatattaagg
    23281 ctcctggaaa atctacaaca tcactatggc gaagaacgct atcgcccatg ttcattgctg
    23341 cgccaacgag cgcttctgga gagcggttat gagtcataag gcctggcaaa atgcccatgc
    23401 aatgtatgag aacgatgcct gcgccaaagc gcttggcatc gacattatct caatggatga
    23461 aggctttgct gtagtgacca tgaccgtcac tgcacaaatg cttaatggtc atcaaagttg
    23521 ccacggcggg cagctatttt cactggctga tactgccttt gcctacgctt gcaatagcca
    23581 ggggttggca gctgtcgctt ctgcctgcac gattgatttt ttacgtccag gctttgccgg
    23641 agacacctta actgcgactg cgcaggtacg tcatcagggc aagcaaaccg gtgtttacga
    23701 catcgaaatt gttaaccaac aacaaaaaac ggttgcgctg tttcgcggta aatctcaccg
    23761 catcggcggc accattacag gagaagcctg atgcgtgaag cctttatctg tgacggaatt
    23821 cgtacgccaa ttggtcgcta cggcggggca ttatcaggtg ttcgggctga cgatctggct
    23881 gctatccctt tgcgggagct gctggtgcga aacccgcgtc tcgatgcgga gtgtatcgat
    23941 gatgtgatcc tcggctgtgc taatcaggcg ggagaagaca accgtaacgt agcccggatg
    24001 gcgactttac tggcggggct gccgcagagt gtttccggca caaccattaa ccgcttgtgt
    24061 ggttccgggc tggacgcact ggggttcgcc gcacgggcga ttaaagcggg cgatggcgat
    24121 ttgctgatcg ccggtggcgt ggagtcaatg tctcgggcac cgtttgttat gggcaaggca
    24181 accagtgcat tttctcgtca ggctgagatg tttgatacca ctattggctg gcgatttgtg
    24241 aacccgctca tggctcaaca atttggaact gacagcatgc cggaaacggc agagaatgta
    24301 gctgaactgt taaaaatctc gcgagaagat caagatagtt ttgcgctacg cagtcagcaa
    24361 cgtacggcaa aagcgcaatc ctcaggcatt ctggctgagg agattgttcc ggttgtgttg
    24421 aaaaacaaga aaggcgttgt aacagaaata caacatgatg agcatctgcg cccggaaacg
    24481 acgctggaac agttacgtgg gttaaaagca ccatttcgtg ccaatggggt gattaccgca
    24541 ggcaatgcct ccggggtgaa tgacggagcc gctgcgttga ttattgccag tgaacagatg
    24601 gcagcagcgc aaggactgac gccgcgagcg cgtatcgtag ccatggcaac cgccggggtg
    24661 gaaccgcgcc tgatggggct tggtccggtg cctgcaactc gccgggtgct ggaacgtgcg
    24721 gggctgagta ttcacgatat ggacgtgatt gaactgaacg aagcgttcgc ggcccaggcg
    24781 ttgggtgtac tacgcgaatt ggggctgcct gatgatgccc cacatgttaa ccccaacgga
    24841 ggcgctatcg ccttaggcca tccgttggga atgagtggtg cccgcctggc actggctgcc
    24901 agccatgagc tgcatcggcg taacggtcgt tacgcattat gcaccatgtg catcggtgtc
    24961 ggtcagggca tcgccatgat tctggagcgt gtttgagcat atcaacctgc gagtacccta
    25021 caatgataac caatacaaag cttgacccga ttgaaaccgc gtctgttgat gagttacagg
    25081 cgttgcaaac acagcgtctg aaatggacgc tcaaacacgc gtatgaaaat gtcccgatgt
    25141 atcggcgcaa attcgacgca gcaggcgtac atcctgatga tttcagggaa ctttcagacc
    25201 tgcgtaaatt tccctgtacc accaaacagg atctgcgcga caactatccc tttgacacct
    25261 ttgccgtgcc gatggaacaa gtggtgcgca ttcatgcttc ttcgggaacc acaggtaaac
    25321 cgacagttgt cggttatacg caaaacgata ttgataactg ggccaatatt gtagcgcgtt
    25381 ctttgcgtgc ggcagggggc tcgcccaaag acaaaattca tgttgcctat ggttacggcc
    25441 tgtttactgg tgggctgggc gcgcactatg gtgccgaacg tctgggcgct acggtgatcc
    25501 cgatgtctgg cgggcagacg gaaaaacaag cgcaactgat ccgcgatttt caaccagata
    25561 tgatcatggt tacgccatct tattgcctta acctgattga agagctggag cggcagttgg
    25621 gcggtgatgc cagcggttgc tcgctgcggg ttggcgtgtt tggtgctgag ccgtggacac
    25681 aggccatgcg taaagagatt gaacggcgtc tggggatcac cgcactggat atttatggcc
    25741 tgtcagaagt gatggggccg ggggtggcga tggagtgtct ggaaactacc gacggtccaa
    25801 ctatttggga agatcatttc tatcctgaaa ttgttaatcc tcatgacggc acaccgcttg
    25861 ccgatggtga acatggcgaa ctgttattca ccacgctgac caaagaagca ttgccggtca
    25921 ttcgttaccg cacgcgtgat ttaacccgac tgttaccagg aacggcgcgg actatgcgcc
    25981 gcatggatcg catcagcgga cgcagcgatg acatgttgat tattcgcggt gtaaatgtct
    26041 ttccgtcaca actggaagaa gagattgtca aattcgaaca tttatcgccg cattaccaac
    26101 tggaggtgaa ccgccggggg catcttgatt cactttctgt gaaagtggag ttgaaagaaa
    26161 gtagcctgac attgacgcat gagcagcgtt gccaggtatg ccaccagctg cgccatcgga
    26221 ttaagtcgat ggtggggatc tctaccgatg tgatgatcgt taactgtggc agtatcccgc
    26281 gttcagaagg caaggcgtgt cgggtgtttg atctgcgcaa tattgttggt gcctgacgtg
    26341 tcgtctggcc ctggtgaggt aaagcgccag ggccagaagt cgatacgacc tgtgctatga
    26401 ttcataaatc acaacaataa caacagactg aatcgaatga gtaaacttga tacttttatc
    26461 caacatgctg taaacgctgt tcctgtcagt ggcacatctt tgatctcctc tctgtatggt
    26521 gattctcttt cccatcgtgg tggtgaaatc tggctgggta gtctggctgc tttgctggaa
    26581 gggctgggat ttggtgagcg tttcgtgcgc actgctttgt ttcgtcttaa taaagaaggc
    26641 tggctggatg tttcccgcat cgggcgacgc agtttctata gcctcagtga taaaggcttg
    26701 cgcctgacgc gacgggcaga aagtaaaatt tatcgcgcag agcaacccgc atgggatggt
    26761 aaatggctcc tgttgctctc ggaaggttta gataaagcga cgctggctga tgtcaaaaag
    26821 cagttgatct ggcaaggttt tggtgcactg gcgcccagcc tgatggcatc gccgtcgcaa
    26881 aaactggccg atgtacagac acttttgcat gaagcgggtg tggcggataa cgtgatttgt
    26941 tttgaagcgc aaataccact ggcgctttct cgtgcagcac tgcgtgccag agtagaagag
    27001 tgctggcatt taactgaaca aaatgccatg tacgaaacct ttattcagtc attccgcccg
    27061 ttggtgcagc tgttaaaaga ggcagcagac gagttaaccc cggagcgtgc atttcatatt
    27121 cagcttttac tgatccattt ttatcgccgt gtcgtcctta aagacccttt gttgccggag
    27181 gagttgcttc cggctcactg ggcagggcat acggcgcgtc agctgtgtat caacatttat
    27241 cagcgcgtag cgcctgctgc tttagcgttc gttagtgaaa aaggtgaaac ctcggtcggt
    27301 gaactgcctt cgccgggtag cctgtatttt caacgttttg gcggtttgaa tattgaacag
    27361 gaggcgttat gccaatttat cagatagacg gtctgactcc ggttgtgcca gaagagagtt
    27421 ttgtccatcc gacagcggta ttgatcggcg atgttattct cggcaagggt gtttacgttg
    27481 ggccaaatgc cagcctgcgt ggcgattttg gtcgtatcgt ggtgaaagat ggcgcgaaca
    27541 ttcaggataa ttgcgttatg cacggttttc ccgagcagga tactgtcgtt gaagaagatg
    27601 ggcatatggg tcatagcgct atccttcacg gctgcattat ccgccgcaat gcattagtgg
    27661 gaatgaacgc ggtagtgatg gatggtgcgg tgattggcga gaacagcatt gttggcgcat
    27721 ccgcatttgt gaaagccaaa gcagaaatgc ctgccaatta cctgattgtc ggcagcccgg
    27781 cgaaactgat tcgtgagctt agtgagcagg agctggcatg gaaaaagcag ggtacgcatg
    27841 agtatcaggt gctggtgaca cgctgtaagc agacgttaca tcaagtcgaa ccattgcggg
    27901 aagttgaacc tggcaggaaa cgcctggtat ttgatgagaa tctgcgaccg aaacagtaac
    27961 gtccagatac tgaacgttct aatttttagc atgtgatacc taaaccgcaa cgctgataca
    28021 ggttgcggtt tttttattgc cgaatgcggc gctttacgcg ttttgttttg gtgtctttca
    28081 ggacaataga gcaactcatc cagtaatctt gtttacgcct tcgcattatt tatctctttt
    28141 cgtttctata ctgatttttc tcaatccgtt ttattacagg gcagggtgcg atgagcagca
    28201 atacatttac tctcggtaca aaatccgtta accgtcttgg ttatggcgcg atgcaactgg
    28261 caggtcctgg ggtttttggt cccccaagag atcgccacgt cgctataacc gtgctgcgcg
    28321 aggcgctggc attgggcgtc aatcacattg ataccagcga cttttatggt ccgcacgtca
    28381 ccaatcagat tatccgcgaa gcgctttatc cttactctga cgacctgaca attgtcacta
    28441 aaattggtgc gcggcgtgga gaggacgcat cctggttgcc cgcattttct ccggcagagc
    28501 tgcaaaaagc ggtgcacgat aatctacgta atctcgggct ggacgtactg gatgtggtta
    28561 acttgcgcat tatgatgggg gatggtcatg gcccagcgga aggatcgatt gaagccagcc
    28621 tgaccgtgct ggcagagatg caacaacaag gcctggtaaa acatattggc ctaagcaacg
    28681 tcacaccgac gcaggttgca gaggcgcgca agattgccga aatcgtctgt gtgcagaacg
    28741 aatacaacat tgctcatcgt gctgatgacg caatgattga tgctttggct cgtgacggca
    28801 ttgcctacgt gccgttcttc ccgctcgggg gctttacacc gctgcaatcc tctacgctgt
    28861 cggatgttgc tgcgagcctg ggtgcaacac ccatgcaggt ggcgctggcg tggctgttac
    28921 aacgttcacc gaatattttg ctgatcccag ggacgtcttc tgttgcgcat ttacgggaga
    28981 atatggctgc tgaaaaattg catctttctg aggaagtgtt gtctacgttg gatggtattt
    29041 cgcgagaata acgaatatac aaaagggaaa gatgcatttc ccttttgttt cttttttaat
    29101 ggcatggagt gcatatgttt aaggccagga tttgcggctg gataggttta cttcccctgt
    29161 ttatgctgag tcttccagta caggcggaac ttcgatgtgt tgcaaataca gttgatattg
    29221 aaccattttt ttctgcggct accgctgaag ataaacaaca agttgaacaa gctatcaaca
    29281 gtagcgtgaa tcttgtcccc ttcggtttat ctgcatcgga ctggaaagtg catcgtggcg
    29341 atttagtggt agaaggtaat atagagagta atcaaaaatt gattgttctt ggtaatttga
    29401 cagtcaaagg taatatttcc actttctctc tttctaatcc atgggttatt ctcggtaacg
    29461 tgacggcaac gaatatagtt acggattcac cactgttaat taccggttcg ataaatgcaa
    29521 gtggactggt atttatcgac tcatattacg ataatccgtc ttcgattaag gggagtatta
    29581 atgcgcgtgg aatatttatc aatgacataa ttgcacctgt tgttgcgtct tcgacaaata
    29641 gtgaattcat ggtccgtgcg agtgacaaaa atgacactga aaatgtcaaa aaagcgctga
    29701 tgataataaa tcctgatgca tattattggg gtctaataaa tgatgaagat gctctgaaag
    29761 aaatttttaa gcgaagcaat attcgcatgg cagggaatgt ctgtaatcag atgaaaaaag
    29821 aagcgctgtt tcgccctaag ccttctcctg agttagtgca ggaattgcaa atgctggatg
    29881 aaggcaacgt tgctgcattt gaaggacgag atattgcgac atttgatctt gccataatgc
    29941 gaactctccc aaggcttaaa ggaatttcag ctaacctacg caaacaactt attaatagca
    30001 atgatgaaca aacgattgaa agtatggcga gatatatgcc tgacaatgag attctggagt
    30061 tgaccgatca gcaattgggt taccagcctg ttgttctggg gttgctcgac cgtgaaccgc
    30121 tctctgtcga aataatgacg cgaatgagtc gtctacctga tggtgtcggt ccgttgaatc
    30181 ttgcgctacg tgaaaatctc cctctggata tcgtaatgac tctggcgaaa agagattggg
    30241 atatgatcat tcaggagctt tataaagatg cgtggttatt gcctgaatcc attattgatg
    30301 gctatatccg cagtgatgat tcctctattc gtcaggtcgg tgctggagga caactcacct
    30361 acaatcaggc aatgcagttg gcgaacgatt catcaaacaa tgttgtcaca agcttagcgt
    30421 tcaagctggc agagatgaaa caccatggtc aattattgcg gatgacgcca caagagagtg
    30481 acaaagttgc agcctattta taccaaaaat tcgagaatga cgatgatcta atacgtgtgt
    30541 tatttttagc attgccagat aacttacagt ttaattttgt taaaaggatg gagaaaaaat
    30601 cccctgccta cttttgctgt cgggatatgc aggtaattca ctctgacgct gctctacaac
    30661 ggttattgac acgtttcaac gatcctgagg ggtggagtaa tctggcgaaa aatcagtatc
    30721 tgagcacgtc gatgaaacaa aaaatttggc aacgtgcttt atcgcatcgg aaaaataatc
    30781 cgaaagcaga ttcagctgcg tatgaaacca gcgctgatat gattttgtct gagcttatca
    30841 gccacggtga agttgatgat cagatgctat taaatgccac cgccctgata cgtttggaag
    30901 actgggactt tttagagagt gcattagtta gttgggataa tttacccgct gttgtcctca
    30961 aggaattaca gcaaaatacg ccacgcaatg atatttgggc gaagtttttt ctgaggcagg
    31021 aaaacagctc ccgtgcgcag gttgatgaag cattacgcgt ctattatgca ctcgaccccg
    31081 atgcgttagc acaactggat gtactggcaa aacaaccgga tcgtatatgg tggagtacac
    31141 tggcgaaaag caatctcaca tttttcaagt tcggcgcact taacaaccgc cacacaccgc
    31201 ctgcagtact ggcggcagaa attgatcccg agtggtggat tgtggcgatg aataatcccc
    31261 gttttccagt tgatgtatta aaggcgaggc tgaaacgtga tcctttgctg gcgttagagc
    31321 ttgttaaccc tgaactggat ttagtccgcc agctggcgct taacggtaag acgcgcgcaa
    31381 tacgggaaca agcgatgaga aaacttgatg agttgtattg atccacaacg taatatttct
    31441 taaccctttg attaattggg gataattatt cctgacagtg agtccccaat accttgatat
    31501 attctgaatt tttaatgaag cggcgtgttg cgatatctcc gtcaggggaa ttgatgcacc
    31561 atagcgcaaa ccgaattatc aaggattgat aatgacgctc taccagataa aaccgctctt
    31621 tcagtcgctg ttaaggccga cgatgttttg gctttacaag caccacgtta cagcgaatca
    31681 catcactctt gctgcactgg cgctttctct tcttaccgga ttgctgttga tgttggtggc
    31741 acaacccatc ctctttatgc tattgcccat cgtgcttttt atccgtatgg cgctcaatgc
    31801 gctggatggc atgttggcgc gtgagtgcaa tcagcaaaca cggcttgggg caattttgaa
    31861 tgagactggt gatgttattt ccgatattgc gctctattta ccatttttat ttttaccgga
    31921 aagtaacgca tcactcgtga tactcatgtt attttgcacc atattgaccg agttttgcgg
    31981 tttactcgcg cagacgatta acggtgttcg cagttatgcc ggtccatttg gcaaaagcga
    32041 tcgcgcgtta atatttggtc tgtggggtct ggccgttgcc atttatccac agtggatgca
    32101 gtggaataat cttttatgga gcattgcttc aattctgctt ctctggactg cgattaatcg
    32161 ttgtcggagt gtgcttctta tgagcgctga aagataatgc tggaaaaatc tctggcaaca
    32221 cttttcgcgt tgttaatttt agcaacgctg ataaaccatt ttctggtatg gcggttaccg
    32281 gagagaaaag aggatgaagt tacattacgt attcgtacct ggtggggcat cgtcatttgt
    32341 ttttcactgg tgatttcagg tccacgctgg atgacgttga cgttttttgc gctgataagt
    32401 tttctggcat tgaaagaata ttgtacgctt atatctgtac attttccgcg gtggttatat
    32461 tgggttattc ctcttaacta tttgtttatc ggttttaatt gctttgagct gtatctatta
    32521 tttatacctt tggctgggtt tctaatatta gccaccgggc aagtttttgt gggggacccc
    32581 tccggttttc tgcataccgt gagcgccatt ttttggggct ggataatgac cgttttcgcc
    32641 ttgagtcatg ccgcctggtt attaatgttg ccaaccacaa atatccaggg cggggcgtta
    32701 ctggtgttat ttcttttggc attaaccgag tcaaacgata ttgcacagta tttatgggga
    32761 aaatcctgcg gcagaagaaa agtggtcccc aaagtcagcc cgggaaaaac gctggaaggt
    32821 ctggtggggg gcgtcatcac caccatgatt gcgtcactga ttattggacc gttgctgaca
    32881 ccgctaaata cattacaggc attattagcg ggtttgttaa ttggtattag tggtttttgc
    32941 ggcgatgtcg tgatgtcagc catcaaacga gatattggtg ttaaagatag tggaaaacta
    33001 ttaccaggac atggcggact ccttgacagg attgactcat taattttcac cgctccggta
    33061 tttttttatt ttattcgcta cagctgttac tgaaggaaaa tagagaaaat ggaaaattca
    33121 cgcatccctg gggaacattt ttttaccacc agtgataata cagcgttgtt ttatcggcac
    33181 tggcccgctt tacagcccgg ggcgaaaaag gtcatcgtct tatttcatcg gggtcatgaa
    33241 cattgtggtc gtctacaaca tctcgttgat gaactggcga tgccagatac tgctttttat
    33301 gcatgggatg cccgggggca tggaaaaact tcggggccgc gtggttatag cccatctctt
    33361 gcgcgttcag tgcaggatgt cgatgaattt gtccgttttg ctgccagcga cagccaggtt
    33421 ggactggaag aggtggttgt gatcgcgcaa agcgtcggcg cagtgctggt tgccacatgg
    33481 gttcatgatt atgcgcctgc aattcgcggg ctggtgctgg cttctccggc ctttaaggtt
    33541 aaattgtatg tgccgctggc acgtcctgcg ctggcgttat ggcatcgtct gcgtggtctg
    33601 ttttttatta attcctatgt gaaaggacgc tatttgaccc acgatcggca acgggtggcg
    33661 agtttcaata atgatccgct gatcacacgg gcgattgccg tcaatatctt gctcgatctc
    33721 tacaaaacgt ctgaacgtat tattagagat gcggcggcga ttacgctccc cacgcaactt
    33781 ctgatatcag gcgatgacta tgtggtgcat cgccaaccgc agattgattt ttatcagaga
    33841 ttacgtagcc ctctgaaaga gctgcatctg ctgccaggct tttatcacga cacgttgggt
    33901 gaagagaaca gggcgcaggc atttgaaaaa atgcaaagct ttattagtcg tttatatgct
    33961 aacaagtcac aaaaatttga ttatcagcat gaagaccgca ctggaccatc agcggatcgc
    34021 tggcggctcc tttcaggtgg acccgtgcca ttatcgccgg ttgatttggc gtatcgcttt
    34081 atgcgaaagg cgatgaaatt gttcggggcg cactctgcgg gcctgcatct cggaatgagc
    34141 accggctttg attcaggcag ttccctggat tatgtctatc aaaatcaacc gcaaggcagt
    34201 aacgcattcg ggcgcttaat tgacaaaatc tacctgaaca gtgttggctg gcgcggtatt
    34261 cgccagcgta aaacccattt acaaatactg attaaacaag ccgttgccga tctccacgcc
    34321 aaaggtttag ccgtccgcgt ggttgacatt gccgcagggc atgggcgcta tgtactggat
    34381 gcgctggcaa acgagcctgc cgtaagcgat attttgttac gtgattacag tgagttaaat
    34441 gttgcacagg ggcaagcgat gattgcccaa cggggaatgt ctgggcgggt gcgttttgaa
    34501 cagggcgatg cgtttaaccc ggaggaactc agcgcgttaa ctccgcggcc tacgatggcg
    34561 attgtctctg gcctgtatga gctttttccc gaaaatgagc aggtaaaaaa ctcactcgca
    34621 ggtcttgcca atgccatcga accgggcggc attctcatct acaccgggca gccgtggcac
    34681 cctcaactgg agatgattgc cggggtgtta accagtcata aagatggtaa accgtgggta
    34741 atgcgcgtgc gttcgcaagg ggagatggat tcactcgtgc gtgatgccgg atttgataaa
    34801 tgcacacaac ggattgatga gtggggtatt tttacggttt cgatggcggt gcgtcgtgat
    34861 aactgaacgt cggaacgtat tgctacaagg cgctggctgg ttattgttgc tggccccgtt
    34921 tttcttcttc acctatggat ctcttaatca gttcaccgcg gttcaggacc ttaacagcca
    34981 tgatatcccc agtcaggtat tcggttggga aacggcgatc ccttttcttc cctggactat
    35041 tgttccttac tggagtctgg atcttttata tggattttcg ctgttcgttt gtagcacgac
    35101 attcgaacag cgccgacttg tccaccggct tattctggca acggtaatgg cctgctgcgg
    35161 ttttttgctc tatccgctga agtttagttt tatccgtcct gaagtgagtg gggtgacggg
    35221 atggctattt tcgcaacttg aactgtttga tctgccttat aaccagtctc cttcgctgca
    35281 tattattctc tgctggctac tttggcgtca ctttcgtcag catctggctg agaggtggcg
    35341 taaagtctgc ggcggatggt ttttactcat cgctatttct actctgacga cctggcagca
    35401 tcattttatt gatgtcatca cagggctggc ggtaggtatg ttgattgact ggatggtgcc
    35461 cgtcgaccgt cgttggaatt atcagaaacc tgatcaacgt cgaatcaaaa tagcactgcc
    35521 ctatgtcgta ggcgcgggct cgtgcattgt attgatggag ctaatgatga tgattcagtt
    35581 atggtggtca gtctggttat gttggccagt attatcgcta ctcataattg gccgtgggta
    35641 cggtgggctt ggcgcgataa caacagggaa agatagtcag gggaaactac cgcccgccgt
    35701 ttactggctg acattgccct gccgcatcgg gatgtggctg tctatgcgtt ggttttgtcg
    35761 tcgcctggag ccggtgagca aaatgactgc tggtgtttat ttaggggcgt ttccacgaca
    35821 tattccggca cagaatgcgg ttctggacgt cacctttgaa ttccctcgcg gacgagcgac
    35881 aaaagatcga ctctatttct gtgtaccgat gctggatctg gtggttccgg aagaggggga
    35941 gctccgacag gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt
    36001 ccattgcgcg ttgggattat cgcgcagtgc gctggtagtg gcggcatggt tgttatgtta
    36061 cggacactgt aaaaccgttg atgaagcgat tagttatatt cgagccagac gttcgcatat
    36121 tgtgcttaag gaagatcaca aagcgatgct gaaattatgg gaaaacaggt aagtggattg
    36181 agatgtggac tgaactacag tccacatcaa gatcgtgtcc ggttatgcag caacaatgct
    36241 gtcgatggcc gctttcgcgt cagactgcgc tttcgctgcc atttccggac cgtatgcgat
    36301 cccttcggcg aagacaaatt tcacatcggt aatgccgata aagccgagga acgtggacag
    36361 atacggcgtc accaggtccg ttggtccatc tttgtggatc ccaccgcggc tggtaataac
    36421 gatggctttt ttacccgtta ccagaccttc cggaccgttc tcggtatagc ggaaagtaac
    36481 gcctgcgcgt gcaaccaggt caaaatagtt tttcaactga gttgagatgt tgaagttata
    36541 catcggtgcc gcaataacga taacgtcgtg ggctttcagc tcggcaatca gctcatcgga
    36601 aagcgccaga gcttcctgct gacgcggagt cagcggcgca tcgctcggac gcagtgcacc
    36661 aaccagttcg ccatccagta ccggaatcgg atttgcagcc aggtcgcgaa cggtgatttc
    36721 atcagcggag tgcttttcgc gccattgttc aacaaaataa tcggacaact gattagactg
    36781 agagtaccct gccaggatgc tggatttaag aactaatacc ttgctcatgg tgtttcctta
    36841 tagatgtttg aatgggcgat gccccgttgc ttgctgacac tttattcaca atcctgccac
    36901 agagatagcg caataaatcg aagcctatgt tcgaatttat tgaacaacgc atagaaagcc
    36961 gcgatgtggt actctatatc tatcatttaa aagaaaatta atcaggcaga ctactgccca
    37021 ctaacgttat gacagaacaa caaaaattga cctttacggc cttgcagcag cggctggatt
    37081 cgctgatgct gcgcgacaga ctgcgttttt ctcggcgtct gcacggtgtt aagaaggtta
    37141 aaaatcctga tgcacaacag gccattttcc aggagatggc gaaagagatt gaccaggcgg
    37201 cagggaaagt cctgctgcgt gaagcggcac gaccggaaat tacttatcct gacaatttac
    37261 cggttagtca gaaaaaacag gacattctcg aagcgattcg tgatcaccag gtggtgatcg
    37321 tcgccgggga aacgggttct ggtaaaacga ctcagttacc gaaaatctgt atggagctgg
    37381 ggcgcgggat taaagggctg atcggccata cccagccgcg tcgcctggcg gcaagaacgg
    37441 tggcgaaccg tattgcggaa gagctgaaaa cggagccggg cggttgcatc ggttacaaag
    37501 tgcgtttcag cgatcacgtt agtgataaca cgatggtcaa gctgatgacc gacggtatcc
    37561 tgctggcgga gatccagcaa gaccgcctgc tgatgcagta cgacaccatc attattgacg
    37621 aagcacacga acgcagcctg aatatcgatt ttctgctcgg ctatttgaaa gagttgctgc
    37681 cgcggcgtcc tgacctaaaa atcattatca cttccgcgac tatcgacccg gaacgctttt
    37741 cgcgccactt taataatgcg ccgattatcg aagtctccgg tcggacctat ccggtggaag
    37801 tgcgctatcg accgattgtt gaagaagccg acgacaccga gcgcgatcag ttgcaggcga
    37861 tttttgacgc cgtagacgaa ctgagtcagg aaagccctgg cgacattctg atctttatga
    37921 gtggtgagcg ggaaattcgc gataccgccg atgcgctgaa caagctaaat ctgcgtcata
    37981 ccgaaatctt gccgctttat gcgcggcttt cgaacagcga acagaaccgc gtgttccagt
    38041 cgcacagcgg acggcgcatt gtgctggcga ccaacgtcgc ggaaacctcg ctgaccgtac
    38101 cggggattaa atacgttatc gaccccggta cagcacgtat cagccgctac agctatcgca
    38161 ccaaagtgca gcgtttgccg attgaaccca tttcgcaggc gtctgccaat cagcgtaaag
    38221 gccgctgtgg gcgtgtgtcc gaagggatct gtattcgtct ctattccgaa gacgatttcc
    38281 tttcgcgccc ggagtttacc gatccggaga ttctgcgtac caacctggcc tcggttattt
    38341 tgcagatgac cgcgctgggg ctgggcgata tcgctgcgtt cccgtttgtc gaagcaccgg
    38401 ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga actgggcgcg atcaccacgg
    38461 atgaacaggc cagcgcctat aaactgacgc cgctcggtcg ccagctctcg cagttgcctg
    38521 tcgatccacg tctggcgcgt atggtgctgg aagcgcaaaa acatggctgc gtgcgtgagg
    38581 cgatgattat cacgtccgcg ctttccattc aggatccgcg cgagcgtccg atggataaac
    38641 agcaggcatc ggacgaaaaa catcgtcgct tccacgacaa agagtctgac ttcctcgcgt
    38701 ttgtgaatct gtggaattat cttggcgagc agcaaaaggc gctttcttcc aacgccttcc
    38761 gtcgcctgtg tcgtaccgat tatctcaact atctgcgcgt gcgcgaatgg caggatatct
    38821 acacccagtt gcgccaggtg gtgaaagaac ttggcattcc ggttaacagc gaaccggcgg
    38881 agtatcgcga aattcacatt gcgttgctga cgggtttact ttcccatatc ggcatgaaag
    38941 atgccgataa acaagaatat accggcgcac gtaacgcgcg tttctccatc tttcccggtt
    39001 ctggtttatt caaaaaaccg ccgaaatggg taatggtggc ggaactggta gaaaccagcc
    39061 gcctgtgggg gcgcattgct gcgcgtatcg acccggaatg ggtggagcct gtcgctcagc
    39121 atttgattaa acgcacctac agcgaacctc actgggaacg ggcgcagggc gcggtgatgg
    39181 caacggaaaa agtcactgtt tatggtttgc cgattgttgc cgcgcgcaag gtcaactaca
    39241 gccagatcga tccggcgtta tgccgtgaac tctttattcg ccatgcgctg gtggaaggtg
    39301 actggcagac gcgtcacgca ttcttccgtg aaaacctgaa actgcgggcc gaagtggaag
    39361 agctggaaca caaatcacgt cgccgcgata ttctggttga tgacgaaacg ttgtttgagt
    39421 tctatgacca gcgcatcagc cacgatgtga tctctgcacg ccacttcgac agctggtgga
    39481 aaaaagtcag ccgcgaaacc cctgatttgc tcaactttga aaaaagcatg ttgatcaaag
    39541 agggcgcaga aaaaatcagc aagctggatt acccgaactt ctggcatcag ggcaatctca
    39601 agctgcgttt gagctatcag tttgagcccg gcgcggatgc tgacggtgtg accgtacata
    39661 ttccgctgcc gttacttaac caggttgagg aaagcgggtt tgaatggcag atccccggtc
    39721 tgcgccgcga actggtgatt gctctgatta aatcgttgcc gaaaccggta cgccgtaatt
    39781 ttgtacccgc accaaactac gccgaagcgt ttttaggccg cgtcacaccg ctggagttac
    39841 cgttgctcga cagccttgag cgcgagttac ggcggatgac cggtgttacc gttgaccgcg
    39901 aagactggca ctgggatcag gtgcccgatc acctgaaaat caccttccgc gtggtggacg
    39961 acaaaaataa gaagctaaaa gaagggcgct cattacagga tctgaaagat gcgctgaaag
    40021 gcaaagtgca ggaaacgctg tccgcggtgg cggatgacgg tatcgagcag agcgggctgc
    40081 atatctggag ctttgggcaa ctgccggaaa gctacgaaca gaagcgcggc aactataaag
    40141 tgaaggcgtg gccggcgctg gtggatgaac gtgacagtgt ggcgatcaaa ctgtttgata
    40201 acccgctgga gcaaaaacag gcaatgtgga acggtcttcg ccgtctactg ctgctaaata
    40261 ttccctcgcc gatcaagtat ttacatgaga agttaccaaa caaagccaag cttggactgt
    40321 actttaaccc gtatggcaaa gtgctggagc tgatcgacga ctgtatctcc tgcggtgtgg
    40381 atcaactgat cgacgccaat ggtggcccgg tctggacgga agaaggcttt gctgcgctgc
    40441 atgagaaagt gcgtgccgaa ctgaacgaca cggtggtaga tattgcgaag caggtcgagc
    40501 aaattcttac ggcagtgttc aatatcaaca aacgtctgaa agggcgggtg gatatgacca
    40561 tggcactggg gctttctgac attaaagcgc agatgggcgg gttggtatat cgcggttttg
    40621 tcactggtaa cggcttcaaa cggctgggcg acacgctgcg atatttgcag gcgattgaaa
    40681 aacggctgga aaaactggcg gttgatccac atcgcgaccg tgctcagatg ctgaaagtcg
    40741 aaaacgtcca gcaggcgtgg cagcaatgga tcaacaaact gccgcctgca cgtcgtgagg
    40801 atgaagacgt gaaagagatc cgttggatga tagaagagtt gcgcgttagt tacttcgctc
    40861 aacaacttgg tacgccttat ccgatttcag ataagcgtat tttgcaggcg atggagcaga
    40921 ttagcggtta accctgctat ttgcctgata aagaaaaaac ccggtaagca tttagcgccg
    40981 ggttttttat taattctaaa acggtaaggg taaaaattca ggaattcaga aaaatacaat
    41041 tctctgctgc aagatgaata atgtttatct acagcatttc cttaaaagat atgtcaggct
    41101 tgcggagtgg cggttaagga catacgactt cctcctttca gagtgctccg cttctcacta
    41161 ttatctcaca cagtattctt aagggaacga taaggaggaa ccatgaacat taccccgttt
    41221 ccgacgcttt cgccggcaac tatagatgcc ataaatgtta tcggacagtg gctggcgcag
    41281 gatgatttct ccggtgaggt gccgtatcag gccgattgcg tgatccttgc aggcaatgcg
    41341 gttatgccga ctatcgacgc ggcatgtaag attgctcgcg atcagcaaat tcctttactg
    41401 attagtggtg gtatcggtca ctcgacaact tttttgtata gcgccatcgc acagcatccg
    41461 cactacaaca ctatccgcac cactggcaga gcagaagcga ccatcctggc ggatatcgct
    41521 catcagttct ggcacattcc gcatgaaaaa atctggattg aagaccagtc aacaaactgc
    41581 ggtgaaaacg cacgctttag catcgcgcta ttgaaacagg ccgtagaacg agttcatacg
    41641 gctatcgttg ttcaggaccc caccatgcag cggcgcacga tggcgacgtt ccgccgtatg
    41701 actggggaca atcccgatgc accacgctgg ttaagttatc ccggattcgt tcctcagtta
    41761 ggaaataacg cagacagtgt aatctttatt aatcagttac aaggattatg gccggttgag
    41821 cgttatctct cactactcac tggcgagctg ccgcgtttac gcgatgatag cgatggctac
    41881 ggtccccgcg ggcgagattt tatcgttcac gttgattttc cggcagaagt catccatgca
    41941 tggcaaacgc tgaaacatga tgcggtgctc atcgaggcga tggaaagtcg ctcgttacgt
    42001 taaaaattgc ccgtttgtga accacttgtt tgcaaacggg catgactcct gacttttatt
    42061 tctgcctttt attcctttta cacttgtttt tatgaagccc ttcacagaat tgtcctttca
    42121 cgatgccgtc tctctgatga ttgatgttaa ttaacaatgc attcaccgaa aataaacata
    42181 taaatcacag gagtcgccca tgtcagtacc cgttcaacat cctatgtata tcgatggaca
    42241 gtttgttacc tggcgtggag acgcatggat tgatgtggta aaccctgcta cagaggctgt
    42301 catttcccgc atacccgatg gtcaggccga ggatgcccgt aaggcaatcg atgcagcaga
    42361 acgtgcacaa ccagaatggg aagcgttgcc tgctattgaa cgcgccagtt ggttgcgcaa
    42421 aatctccgcc gggatccgcg aacgcgccag tgaaatcagt gcgctgattg ttgaagaagg
    42481 gggcaagatc cagcagctgg ctgaagtcga agtggctttt actgccgact atatcgatta
    42541 catggcggag tgggcacggc gttacgaggg cgagattatt caaagcgatc gtccaggaga
    42601 aaatattctt ttgtttaaac gtgcgcttgg tgtgactacc ggcattctgc cgtggaactt
    42661 cccgttcttc ctcattgccc gcaaaatggc tcccgctctt ttgaccggta ataccatcgt
    42721 cattaaacct agtgaattta cgccaaacaa tgcgattgca ttcgccaaaa tcgtcgatga
    42781 aataggcctt ccgcgcggcg tgtttaacct tgtactgggg cgtggtgaaa ccgttgggca
    42841 agaactggcg ggtaacccaa aggtcgcaat ggtcagtatg acaggcagcg tctctgcagg
    42901 tgagaagatc atggcgactg cggcgaaaaa catcaccaaa gtgtgtctgg aattgggggg
    42961 taaagcacca gctatcgtaa tggacgatgc cgatcttgaa ctggcagtca aagccatcgt
    43021 tgattcacgc gtcattaata gtgggcaagt gtgtaactgt gcagaacgtg tttatgtaca
    43081 gaaaggcatt tatgatcagt tcgtcaatcg gctgggtgaa gcgatgcagg cggttcaatt
    43141 tggtaacccc gctgaacgca acgacattgc gatggggccg ttgattaacg ccgcggcgct
    43201 ggaaagggtt gagcaaaaag tggcgcgcgc agtagaagaa ggggcgagag tggcgttggg
    43261 tggcaaagcg gtagagggga aaggatatta ttatccgccg acattgctgc tggatgttcg
    43321 ccaggaaatg tcgattatgc atgaggaaac ctttggcccg gttctgccgg tggtcgcatt
    43381 tgacacgctg gaagaggcta tctcaatggc taatgacagt gattacggcc tgacctcatc
    43441 aatctatacc caaaatctga acgtcgcgat gaaagccatt aaagggctga agtttggtga
    43501 aacttacatc aaccgtgaaa acttcgaagc tatgcaaggc ttccacgccg gatggcgtaa
    43561 atccggtatt ggcggcgcag atggtaaaca tggcctgcat gaatatctgc agacccaggt
    43621 ggtttattta cagtcgtaat gagtaaaaga ggcggaggtt ttttcctccg cctgtgcgcg
    43681 tcagagttta gcgaattttt cgagggtgcg aataagctgc gtgacgaagc catattcgtt
    43741 atcgtaccag gcgaccgttt tcaccagttg taaatcgccc acggcggtaa tttccgtttg
    43801 cgtggcatca aacaccgaac cgaaatggct gccaatgata tcggaagaga ctatttcttc
    43861 atcggtataa ccaaatgact cgttattggt ggttgcttgt ttaagtgcgt tattcacctc
    43921 ttcggcagtc actttttttc cgagaatcga taccagttca gtgaccgaac ctgttttcac
    43981 cggcacgcgt tgcgcatgac ctttcagttt gccgctcagt tccgggatca ccagaccaat
    44041 ggcttttgcc gcccccgtag tgtggggaat gatattttct gccgctgcgc gtgaagcacg
    44101 taaatcttta ccacgtgggc catccaccag tgactgggtg ccagtatagg catgaatggt
    44161 cgtcatcgtg ccgacttcta tcccgaaact gtcatgcaag gctttggcca tcggcgcaag
    44221 acagttagtg gtgcatgacg ccacggaaac aatggtgtcg ttgccatcca gagtgtcgtc
    44281 attgacgtta taaacgatag ttttcatttc accggcaggg gcggaaatca acaccttctt
    44341 cgcaccagca tcaagatgcg cctgcgattt ctcggcggag gtataaaagc cagtacattc
    44401 gacaatgatt tctgcccctt tcgctttcca cggaatattt ttagcctctt tttcggcgta
    44461 aaccgcgata cttttcccat caacgataag tgaatcttcc gtaaaatcaa cgctccaggg
    44521 gaacggacca tagtttgaat catgtttcag cagataggcg agaatttttg gggaagtgag
    44581 atcattaata gcgacaacgt ctatgttgct tttgacttca agtaatcgac gcaacaccag
    44641 tcgaccgata cgaccaaaac cgttaatacc aactttactc atggttttct cctgtcagga
    44701 acgttcggat gaaaattgat cctttccagg cttagcccag gattgtggga tgggcaatct
    44761 ccattcttac agtgaaacgt aacgtactga aaacgggtga acaatattta atgaaatttt
    44821 gagaaaagcc cgttatgtta acggaaaatt atgttaaagc aggaaatgtt atggaaaata
    44881 aatactcaag gttacaaatc agcattcact ggctggtctt tttgctggtt atcgcagcgt
    44941 attgcgcaat ggagtttcgt ggtttcttcc cacgtagcga tcggccactc atcaacatgg
    45001 ttcatgtttc ctgtggtatc tcaattctcg tgctgatggt ggttcgtctt ttgttaagac
    45061 tgaaataccc aaccccgccg attataccta agccaaaacc gatgatgacg ggactggcgc
    45121 atttgggaca tttggtgatt tatctcctgt ttattgcgct gccagtgatt ggtttggtga
    45181 tgatgtataa ccggggcaac ccgtggtttg cgtttggttt gacgatgcct tacgcttcag
    45241 aggccaattt cgaacgggtt gatagcttaa agtcgtggca tgagacgctg gcgaatctgg
    45301 gatattttgt catcgggttg cacgctgcgg ctgcactggc acaccactat ttctggaagg
    45361 acaacacact tctacgcatg atgccgcgta aacgttcctg aaggatattt aaagaaaacg
    45421 cctgtactaa aaccgacccg tggtacaggc gaagaatacg ggtctacatc ggaagcgcct
    45481 attatattta tttgtatgac aaataaaacc ataatccttg cccatacgtc catctggctt
    45541 atttttaatc aattcacccg atctttgatc tcatcaacgg tatcaaaata aaccagcgta
    45601 taacgttcaa atcgaacata agattcgata aaccatggag gttatatgaa aaaactggca
    45661 cttattttgt ttatgggaac gcttgtttcc ttttatgccg atgccgggcg caaaccctgt
    45721 tctggttcga aaggggggat ctcacactgt acggcaggcg gcaaatttgt ctgtaatgat
    45781 ggttctatta gtgcctcgaa aaaaacatgc actaactgaa gtgtaaaagg ggtgccatga
    45841 gaaaatgaat ctgctagtca gatgcgcggg gaaaatcccc gcgcttgccc ttacctggac
    45901 gtgcaggcca tgagcgcagc aacctcctta tcaccgtccc ggaaccgcag ttcgtagagc
    45961 gtttgtcggg tcaggagtgt gaatgtcaga atcgtaatgc agataatgag cagacacacc
    46021 accagagggt tgtgcttcat agcctttctc cttgccggat ggcgggtaag aggctaagat
    46081 ctgaattgct aggttcattc gttggcctcg gttgatagaa atatcggtcg gggccttcgt
    46141 ctttctgatt cccggttagc ctgaaaacag aaagtctcag gcacccgcag gcatcctatg
    46201 aggtttcctt agggacgaaa ataatcactt cacgaaattg cgtgctgttt tccagaattt
    46261 ttcgtcattc gggttagcca gtttagccat tcgttactct ctttattcca atagcattaa
    46321 ttttctctgc aataattgtt gtaaaaatgt gacgcaaaga ggtttttggt cataagtaat
    46381 taccgtcaag tgccgatgac tttctatcag gagtaaacct ggacgagaga caacggtaat
    46441 gaatacaact ccctcacatc gattaggttt tttgcatcac atcaggctgg ttccgttatt
    46501 tgcctgcatt cttggcggta tcttagttct attcgcatta agttctgctc tggctggcta
    46561 tttcctctgg caggccgatc gcgatcagcg tgatgttact gcggagattg agattcggac
    46621 cgggttagcg aacagttcag attttttgcg ttcagcccgg atcaatatga ttcaggccgg
    46681 ggctgcgagt cgtattgcgg aaatggaagc aatgaagcgc aatattgcgc aagccgaatc
    46741 ggagattaaa cagtcgcagc aaggttatcg tgcttatcag aatcgaccgg tgaaaacacc
    46801 tgctgatgaa gccctcgaca ctgaattaaa tcaacgcttt caggcttata tcacgggtat
    46861 gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa gcgattatta atcatgaaag
    46921 tgagcagatc cgaccgctgg ataatgctta taccgatatt ttgaacaaag ccgttaagat
    46981 acgtagcacc agggccaacc aactggcgga actggcccat cagcgcaccc gcctgggtgg
    47041 gatgttcatg attggcgcgt ttgtgcttgc cctggtcatg acgctgataa catttatggt
    47101 gctacgtcgg atcgtcattc gtccactgca acatgccgca caacggattg aaaaaatcgc
    47161 tagtggcgat ctgacgatga aggatgaacc ggcgggtcgt aatgaaatcg gtcgcttaaa
    47221 tcgtcattta cagcaaatgc agcattcact ggggatgaca gtagggactg ttcgacaggg
    47281 tgcggaagag atttatcgtg gcaccagcga aatttcagct ggcaatgcgg acctgtcatc
    47341 tcgcaccgaa gaacaagcgg cggcaatcga acaaactgcc gccagcatgg agcaactcac
    47401 tgcgacggtg aaacagaatg cggataacgc gcatcatgcc agcaaactgg cgcaagaggc
    47461 ttctattaaa gccagcgatg gcgggcagac ggtttccggt gtagtaaaaa cgatgggcgc
    47521 tatctccacg agttcgaaga aaatttctga gatcaccgcc gtcatcaaca gtattgcttt
    47581 ccagacgaat attctggcac tgaatgctgc cgttgaagcc gcgcgagcgg gtgagcaagg
    47641 gcgtggattt gccgttgtcg ccagcgaagt acggacactc gcaagccgca gcgctcaggc
    47701 ggcgaaagag attgaaggct tgatcagtga atcagtcagg ttaattgacc tggggtcgga
    47761 tgaggtggca acggcaggga aaaccatgag cactattgtt gatgccgtcg cgagtgtcac
    47821 acatatcatg caggaaatcg ccgccgcctc ggatgaacaa agtagaggca taactcaggt
    47881 tagccaggcg atttctgaaa tggataaggt gacgcaacag aatgcttctc tggtagaaga
    47941 ggcctcagca gcggcggtgt cccttgaaga acaggctgca cgattaactg aggcggtgga
    48001 tgtattccgt ctgcacaaac attctgtgtc ggcagaacct cgcggagcgg gtgaaccagt
    48061 tagtttcgct acggtgtgaa aatgttcaag gagggatcga cagatccctt cacctttcag
    48121 aacggcattg attttcgaat agcgttaatc atcaactggc aaccagaaga gaacgtcgca
    48181 tctacgcggg tcagtattcc aatcggttcg cctgcaccat gtcccggaac aggcagggcc
    48241 accagcgtgg catgacgcag gtcgtctttt acagcgccag aagggacaaa ccacacgtaa
    48301 tcgtattcaa ccgtaagttg acgagatagc gaagcagaca gcgtttcgat acaacccgaa
    48361 ggaattttac agccctggct ctgcactaat gcatctgaat gctggcgtgg cgcagtgcct
    48421 tctggtgata caacgaccgg ccattccagc acccggctta gcgttacgtt ctcctgaagt
    48481 agcgggtgat tagggcggac aaccagcttc aacgattcaa gaaacagcag ttcgtaatta
    48541 agcccggtca tcagttcagg atctgacatc cgaccaatgc cgatatcgat ttccccggtt
    48601 tttaaacccg ccagaatcat agggttactc attgtcgcaa cttgcaaggt cgtctctttt
    48661 tgttgctgat gaaactgacc tataaccgaa ggtaatatcc ccagtgccgc agtaggtagt
    48721 gcaccaaccc tgacgacatc attattaaga ccttctttac gatgaagcga ctgtccggca
    48781 gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc gggtaaggta
    48841 agttgcgccc cctgacgacc acgctcaaac aagcgagcgc cagtcagctg ctccagttca
    48901 ttcaatgtct tagagagcgc aggttgactc aaattaaggg tttcagccgc gcgccccaaa
    48961 gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat gcgctgacta
    49021 aacagactat ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct gacaagtgaa
    49081 gttgtttgat tatgataact tgattgcaaa atattattaa caattaaagc aattatgtta
    49141 cagcaaaatg gataatattg atgttttcgc ggcgagatca cagtttgtaa attcttcccg
    49201 caagagtgaa tgcggttacc tacactccag attactgacc actggaggca gacactatgg
    49261 cgaacagcat cacggcggat gagattcggg aacagttttc gcaggcaatg tcagccatgt
    49321 accagcaaga agttccgcaa tatggcacgc tgctggaact ggtagctgat gtgaatctgg
    49381 ctgtgctgga aaacaatcct caactgcacg aaaaaatggt aaatgcagac gagctggcgc
    49441 gactgaatgt tgaacgtcat ggggcgattc gcgttgggac tgcacaagag cttgctactc
    49501 ttcggcggat gtttgccatt atggggatgt acccggtgag ctattacgat ctctcgcagg
    49561 caggggtgcc ggtacattcg acagcatttc ggcccattga tgatgcttct ctggcgcgta
    49621 atcccttccg cgtttttacc tccttactcc gccttgagct tatcgagaac gaaattttgc
    49681 gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt caccccacgt tgtcgacaac
    49741 tgttagagga atatgagcag cagggcggtt ttaacgaaac acaggcacag gagtttgtgc
    49801 aggaagccct ggaaacgttt cgctggcacc agtcagcaac ggtagatgaa gaaacctatc
    49861 gcgccttgca caacgaacat cggttgattg ctgatgtggt ctgttttcct ggatgccata
    49921 tcaaccacct gacaccacgt acgctggata ttgaccgggt gcagtcgatg atgcctgaat
    49981 gcggaattga acccaaaatt ctcatcgagg ggccgccgcg ccgcgaggta ccgattttac
    50041 tacgccagac cagctttaaa gcactggaag agacggtgtt gtttgcgggg cagaaacagg
    50101 gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg cgtggcatta acgccgaaag
    50161 ggcgacaact gtatgatgat cttctgcgta acgctggaac cgggcaggat aatctcactc
    50221 accaaatgca tttacaggaa accttccgca cttttcctga cagtgagttt ttaatgcgtc
    50281 agcaagggct ggcatggttc cggtaccgtc tgacgccttc aggtgaggcg catcgtcagg
    50341 cgattcatcc cggagacgat ccacagccct taattgaacg tggttgggtc gcggcgcaac
    50401 ccattaccta tgaagatttc ttgcccgtta gcgcggcggg gatcttccag tcaaatctgg
    50461 gtaatgaaac gcagacacgc agtcacggta atgccagtcg cgaagcattt gagcaggcgt
    50521 tgggttgtcc ggttttggat gagttccagc tttaccagga agcggaagaa cgcagtaaac
    50581 gtcgctgtgg tttgctttaa attctgacca tccgcctttg caaaaatttg cctgatttta
    50641 caaacgaatc aggctcatcc catcgacata aaaaaaatgc cgatttatgc atattctctc
    50701 agttcaacaa ttggattatt aataaatatt gtctagagtg agcggtcata aataagtact
    50761 ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa caccagaagg actcactttc
    50821 aggtatggat cgtagacgat ttattaaagg ttcaatggct atggccgccg tgtgcggtac
    50881 cagcggcatt gcttctcttt tttctcaggc ggcattcgcg gcagattctg atattgccga
    50941 cgggcaaacc cagcgttttg acttctccat tctacagtca atggcgcacg acttagcgca
    51001 aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg ctggcgacaa tgacgccgca
    51061 ggcttataac agtattcaat acgacgccga aaaatcgctc tggcataacg ttgagaaccg
    51121 tcaactggac gctcagttct tccatatggg aatgggattc cgtcgccgcg ttcgtatgtt
    51181 ttctgtagat ccagcaacac atctggcgcg tgaaattcac tttcgcccgg agttgttcaa
    51241 atacaacgat gcgggtgttg atacaaaaca attagaaggg caaagcgatc tcggctttgc
    51301 cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat catttctcgg
    51361 cgcgagttat ttccgcgccg ttgatgatac atatcaatac ggtttgtcgg cccgcggcct
    51421 ggcgatcgac acttacactg acagtaaaga agagttcccc gactttaccg ccttctggtt
    51481 tgatacggta aaaccggggg caactacctt taccgtttat gcgttgctcg atagcgccag
    51541 cattactggt gcctataagt tcactatcca ttgtgagaaa agtcaggtga ttatggatgt
    51601 ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg ggcattgcgc cgatgaccag
    51661 tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat acaattcatc cgcaaattca
    51721 tgactctgat cgtctgtcca tgtggcgggg caacggcgag tggatttgcc gtccgctgaa
    51781 taatccgcaa aaattgcagt tcaatgctta caccgacaac aacccgaaag ggtttggttt
    51841 attgcaactg gatcgtgact tctcccatta tcaggacatt atgggctggt ataacaaacg
    51901 cccaagtctg tgggtggagc cgcgtaacaa gtggggtaag ggcaccatcg gcctgatgga
    51961 aatcccaaca acgggcgaaa cgctggataa cattgtctgc ttctggcagc cagaaaaagc
    52021 tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg tactggagtg cgcaaccgcc
    52081 tgttcattgc ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg gtttcccgga
    52141 aggttgggca ccaggtgaac actatcccga aaaatgggcg cgtcgttttg ccgtcgattt
    52201 cgttggtggt gatctgaaag ctgccgcacc aaaaggcatt gagccggtga ttacgctttc
    52261 cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa cccattgatg gttatcgtat
    52321 tcagtttgac tggtatccga cttcggactc cactgatccg gtcgatatgc ggatgtatct
    52381 gcgttgtcag ggcgacgcta tcagtgaaac atggctgtat cagtatttcc cgccagcgcc
    52441 ggataaacgt cagtatgttg acgaccgcgt gatgagttaa tcgttttttc ttcggcacct
    52501 tcttcgggag gtgccgtctg gttaaacacg atcccgctcg catttttccc taagttaaat
    52561 gagtaatctg atggtgtgta tttcagatac accttgtcag ccactaacag ggagtgcgta
    52621 tgtttccaga ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct cgctttatgt
    52681 ccttgttcga taaacacaat aaacttgatc atgaaattgc cagaaaggaa ggttccgacg
    52741 gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca aaagctacag ttaaaagatg
    52801 agatgctcaa aatcctgcag caggagagcg tcaaagaggt gtaaactttc ctaagccgcc
    52861 agccaggcgg cttttttaac aacagcatgg attgactgga gataagatga ctgaaacgat
    52921 aaaagtaagc gaatcacttg aattacatgc tgttgcggaa agtcacgtca cacctcttta
    52981 tcagttaatc tgtaaaaata aaacctggtt acagcagtcg ctaaactggc cgcagttcgt
    53041 tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc atcaacgcgg
    53101 ctatgccaaa atgttcatga ttttcaaaga agatgaactt atcggcgtta tctcgtttaa
    53161 tcgtattgaa ccactgaata aaactgctga aatcggctac tggctggacg aatctcatca
    53221 ggggcagggg atcatttctc aggcgctgca ggcattgatt catcattacg ctcagtctgg
    53281 tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca accaggtcgc
    53341 tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag gctgagttcc tgaatgatgc
    53401 ctatgatgat gtgaacttat acgcgcgtat tatcgattca taataaccct gccagcggcg
    53461 ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc acggacatga accgtgtcgc
    53521 catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac atggttttca attatcaccg
    53581 cacctgaaat acggctctca ccttgtatta caacgtgctc gtcgagcaga atcggtccac
    53641 cacgtaccac ggcatttccg ccaatcaaga catgatgttt taacacacaa ttaccttcca
    53701 caatggcata ttccgccacc tgcgaactgt aatgaatcgt cggaatggca tcttcttcta
    53761 tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca atcgcatagc cagacgttgt
    53821 tctcttcatt accttcaaca ctggcaaaat caaaaacttc ggcgcgatgt tcaataaaag
    53881 cataccggac gacggcatcg ccataaattt gtgcctggtg gacaatacgc gaggcgctaa
    53941 cccttgcgcg atcataaatt tggagcagga gttgatggtc gggcgttaag ccttgtgcgg
    54001 cgacgatcat agaatgttga tcaattaagg cgtgaccaaa tattctacat tgcccgtaaa
    54061 ctaatgagtc atgaatggtg acactgtcac ttatgtatgc accctggctg atttcactat
    54121 tatcgatcca gacattatcc gttgcgtaaa cctctcccca cagtacgctg gtgccggtga
    54181 tgcgcgtatt accggaaatc accgttccgc cgaacgcaat agcattctgg tcgtaaatcc
    54241 agcaatttcc ttcctgtgct aacaccgttt cgcgatcgat ccagccgcca gcggttcctg
    54301 ctatcacatc gttaaaatcg ctaatggcga taatctgccg taataacaca ttttttttag
    54361 tgccatcttc ctgataacta aaggctcgct gttcttcact tagacgatat ttgcgcatag
    54421 cgttttccca caggtgactt actataaccg tagcaaattc tgcggctctg gctatgctcg
    54481 agaaaattcc ataaaatgca tttcaaatat actttataaa ttaaacaaaa tgagtaagaa
    54541 gatgcagagc gataaagtgc tcaatttgcc ggcaggctac tttggtattg tgttggggac
    54601 gatagggatg ggatttgcct ggcgctatgc cagccaggtt tggcaggtca gccactggtt
    54661 aggggatggg ctggtgattc tggcgatgat catctgggga ttattaacta gcgcatttat
    54721 tacccgactc atacgctttc cgcatagcgt gctggcggaa gttcgccatc cagtgctgag
    54781 cagttttgtg agtttgtttc cggcaacgac gatgctggtg gcgattggtt ttgttccgtg
    54841 gtttcgccca ctggcggtgt gcctgttcag ttttggtgtc gtggttcagt tggcttatgc
    54901 cgcctggcaa actgcgggat tatggcgcgg atctcaccct gaagaagcta ccacgcctgg
    54961 actgtatctg ccgacagttg ccaacaactt tatcagcgca atggcctgtg gtgcgttggg
    55021 ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc taagccttga
    55081 accggtgatc ttgcagcgtc tgcgcagttc gggagaatta cccacggcac tgcggacatc
    55141 actcggcatt cagctcgctc ctgcgctggt ggcttgtagt gcctggctga gcgtcaacgg
    55201 cggcgagggt gacacgctgg cgaaaatgct tttcggttat ggactgctgc aactgctgtt
    55261 tatgctacgt ctgatgccat ggtatctctc ccagccattt aatgcttcat tctggagttt
    55321 ctcgttcggc gtatctgcac tggcaaccac cggtttgcat ctggggagtg gcagcgataa
    55381 tggatttttc catacgctgg cggtgccgct gtttatcttt accaatttta ttattgcaat
    55441 actgctcatc cgtacttttg cgcttctgat gcagggaaaa ttgttagtca gaaccgagcg
    55501 cgccgtttta atgaaagcag aggacaaaga atgatcattc gtgacgaaaa ctattttact
    55561 gataaatatg aattaacccg cacacactct gaagtactgg aagcggtgaa agtggttaaa
    55621 ccgggtaaaa cgctggatct gggctgtggc aatggtcgta acagtcttta cctggcagcc
    55681 aatggttatg atgttgacgc atgggataaa aatgccatga gtatcgccaa cgtcgagcgc
    55741 attaaatcca ttgaaaatct ggataattta cacacccgag tcgttgatct gaataacctc
    55801 acatttgata gacagtacga ttttattctt tcgactgtgg tactgatgtt ccttgaggct
    55861 aaaaccatcc cagggctgat tgtcaatatg caacgttgca ctaaacctgg tggttacaac
    55921 ctgattgtgg cggcgatgga taccgctgat tatccatgta ccgtcggctt cccgtttgcc
    55981 ttcaaagagg gagaattacg tcgatattac gaaggctggg agagggtgaa atacaatgaa
    56041 gacgtcggcg aactgcaccg caccgacgcc aacggtaatc gtattaaact gcgtttcgcc
    56101 acgatgctgg cacgtaaaaa atgacccgct aagaataagg cgcgtgaaaa ttccccacgc
    56161 tgagatgatt tactgttctt cttttcggta agcatatttt ttatcgaagg gatgtgaaat
    56221 taatcacagt agtcgaagtt ttttagcagc ttaacttact gaaatttaac tactgatgat
    56281 tgacttagcc cctttttcgg cattgactat gtcgtctgaa aaggggctga aaaatttatt
    56341 ttcaccaaca ctttttttgc cacaacacga agcggcgctt tttgctataa cttagaaagt
    56401 aatataatca tctcaggaaa ctattcatgc gtaccacatc atttgcgaaa gttgcagctt
    56461 tatgcggctt gttggctctg tctggttgtg cctctaaaat cacccagcca gataaatatt
    56521 ctggtttttt aaacaattac tctgatttaa aagaaacaac ctcagcaaca ggtaaacctg
    56581 ttttacgttg ggtagacccg agttttgatc aaagcaaata tgacagcatc gtctggaacc
    56641 caatcactta ttatccggta ccaaaacctt ctacccaggt agggcagaaa gttctggata
    56701 aaattttgaa ctataccaac accgaaatga aagaagcgat agcgcagcgt aaaccactgg
    56761 ttaccaccgc tgggccgcgt agtctgattt tccgtggggc cattaccggt gtagatacca
    56821 gcaaagaagg gctgcaattc tatgaagtgg ttcctgttgc attagtggtt gcggggacac
    56881 aaatggctac aggccaccgt accatggata ctcgcctcta ttttgaaggt gagctgattg
    56941 atgcagcgac taataaaccg gttatcaaag tcgttcgtca gggcgaaggt aaagacctga
    57001 ataacgaaag tacgccaatg gctttcgaaa atattaaaca agttattgat gacatggcga
    57061 ccgatgccac catgtttgac gttaacaaaa agtagttcca gacgcgccat cgttagatgg
    57121 cgctttttat ccggtgcgcc gtaaaacccc atccttcagg gcggggatat aaggcgcggt
    57181 tttccacctg accaggtgtt tgctgttgtt caatgtattg gcggatgacg gatattggcg
    57241 cacctccgca gctactggca aaatagccag gactccacaa aacgcctttg tagtaatacc
    57301 tgactgcaat atctggtcga tctcgtcgca gtaacctact cgatacgcct ttgaggctgt
    57361 ttaccagact ggatatcgcc agtttgggag gatagttaat taacaaatgg acgtgatctg
    57421 gttcgccatc catttaaacc agttcggctt caaaatcagc acatacattt gaaaagtaag
    57481 tgcgtagttt ttctgtcgcg tcatagtcaa aaatctgacg tcggtatctg gtgacaaaga
    57541 ccaggtgaac atgcatcagg aaaacacaat gcttccccgt cggatatcgg tttctttatt
    57601 catagaccaa agtatcattt ggcctgtgaa acgattacag gcatttaaat tccagttaag
    57661 acccggtggt caacaggag
//
LOCUS       DAZFYC010000015        57109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFYC010000015 DAZFYC010000000
VERSION     DAZFYC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.015"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(86..1276)
                     /gene="aspC"
                     /locus_tag="ACHKW5_001080"
     CDS             complement(86..1276)
                     /gene="aspC"
                     /locus_tag="ACHKW5_001080"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO7510513.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1461..2549)
                     /gene="ompF"
                     /locus_tag="ACHKW5_001081"
     CDS             complement(1461..2549)
                     /gene="ompF"
                     /locus_tag="ACHKW5_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO7510514.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(3151..4551)
                     /gene="asnS"
                     /locus_tag="ACHKW5_001082"
     CDS             complement(3151..4551)
                     /gene="asnS"
                     /locus_tag="ACHKW5_001082"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO7510515.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(4720..5922)
                     /gene="pncB"
                     /locus_tag="ACHKW5_001083"
     CDS             complement(4720..5922)
                     /gene="pncB"
                     /locus_tag="ACHKW5_001083"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO7510516.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIVHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDCPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            6188..8800
                     /gene="pepN"
                     /locus_tag="ACHKW5_001084"
     CDS             6188..8800
                     /gene="pepN"
                     /locus_tag="ACHKW5_001084"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO7510517.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(9007..9774)
                     /gene="ssuB"
                     /locus_tag="ACHKW5_001085"
     CDS             complement(9007..9774)
                     /gene="ssuB"
                     /locus_tag="ACHKW5_001085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7510518.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(9771..10562)
                     /gene="ssuC"
                     /locus_tag="ACHKW5_001086"
     CDS             complement(9771..10562)
                     /gene="ssuC"
                     /locus_tag="ACHKW5_001086"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO7510519.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(10573..11718)
                     /gene="ssuD"
                     /locus_tag="ACHKW5_001087"
     CDS             complement(10573..11718)
                     /gene="ssuD"
                     /locus_tag="ACHKW5_001087"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO7510520.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(11715..12674)
                     /gene="ssuA"
                     /locus_tag="ACHKW5_001088"
     CDS             complement(11715..12674)
                     /gene="ssuA"
                     /locus_tag="ACHKW5_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO7510521.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADTRAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(12667..13242)
                     /gene="ssuE"
                     /locus_tag="ACHKW5_001089"
     CDS             complement(12667..13242)
                     /gene="ssuE"
                     /locus_tag="ACHKW5_001089"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO7510522.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            13598..14140
                     /locus_tag="ACHKW5_001090"
     CDS             13598..14140
                     /locus_tag="ACHKW5_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510523.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            14223..14924
                     /gene="elfD"
                     /locus_tag="ACHKW5_001091"
     CDS             14223..14924
                     /gene="elfD"
                     /locus_tag="ACHKW5_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7510524.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            14949..17549
                     /gene="elfC"
                     /locus_tag="ACHKW5_001092"
     CDS             14949..17549
                     /gene="elfC"
                     /locus_tag="ACHKW5_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO7510525.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            17540..18610
                     /gene="elfG"
                     /locus_tag="ACHKW5_001093"
     CDS             17540..18610
                     /gene="elfG"
                     /locus_tag="ACHKW5_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510526.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            18622..19164
                     /gene="ycbU"
                     /locus_tag="ACHKW5_001094"
     CDS             18622..19164
                     /gene="ycbU"
                     /locus_tag="ACHKW5_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510527.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            19172..19687
                     /gene="ycbV"
                     /locus_tag="ACHKW5_001095"
     CDS             19172..19687
                     /gene="ycbV"
                     /locus_tag="ACHKW5_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510528.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            19653..20390
                     /gene="ycbF"
                     /locus_tag="ACHKW5_001096"
     CDS             19653..20390
                     /gene="ycbF"
                     /locus_tag="ACHKW5_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7510529.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            20501..21511
                     /gene="pyrD"
                     /locus_tag="ACHKW5_001097"
     CDS             20501..21511
                     /gene="pyrD"
                     /locus_tag="ACHKW5_001097"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO7510530.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            21685..22227
                     /gene="zapC"
                     /locus_tag="ACHKW5_001098"
     CDS             21685..22227
                     /gene="zapC"
                     /locus_tag="ACHKW5_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO7510531.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(22224..23333)
                     /gene="ycbX"
                     /locus_tag="ACHKW5_001099"
     CDS             complement(22224..23333)
                     /gene="ycbX"
                     /locus_tag="ACHKW5_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO7510532.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            23577..25685
                     /gene="rlmKL"
                     /locus_tag="ACHKW5_001100"
     CDS             23577..25685
                     /gene="rlmKL"
                     /locus_tag="ACHKW5_001100"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO7510533.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            25696..27603
                     /gene="uup"
                     /locus_tag="ACHKW5_001101"
     CDS             25696..27603
                     /gene="uup"
                     /locus_tag="ACHKW5_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7510534.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQSVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            27733..28986
                     /gene="pqiA"
                     /locus_tag="ACHKW5_001102"
     CDS             27733..28986
                     /gene="pqiA"
                     /locus_tag="ACHKW5_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO7510535.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VADLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            28991..30631
                     /gene="pqiB"
                     /locus_tag="ACHKW5_001103"
     CDS             28991..30631
                     /gene="pqiB"
                     /locus_tag="ACHKW5_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO7510536.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            30628..31191
                     /gene="pqiC"
                     /locus_tag="ACHKW5_001104"
     CDS             30628..31191
                     /gene="pqiC"
                     /locus_tag="ACHKW5_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO7510537.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQITVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            31447..31614
                     /gene="rmf"
                     /locus_tag="ACHKW5_001105"
     CDS             31447..31614
                     /gene="rmf"
                     /locus_tag="ACHKW5_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO7510538.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(31684..32202)
                     /gene="fabA"
                     /locus_tag="ACHKW5_001106"
     CDS             complement(31684..32202)
                     /gene="fabA"
                     /locus_tag="ACHKW5_001106"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO7510539.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(32271..34031)
                     /gene="ycbZ"
                     /locus_tag="ACHKW5_001107"
     CDS             complement(32271..34031)
                     /gene="ycbZ"
                     /locus_tag="ACHKW5_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7510540.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            34217..34669
                     /gene="matP"
                     /locus_tag="ACHKW5_001108"
     CDS             34217..34669
                     /gene="matP"
                     /locus_tag="ACHKW5_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO7510541.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(34745..35785)
                     /gene="ompA"
                     /locus_tag="ACHKW5_001109"
     CDS             complement(34745..35785)
                     /gene="ompA"
                     /locus_tag="ACHKW5_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO7510542.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(36142..36651)
                     /gene="sulA"
                     /locus_tag="ACHKW5_001110"
     CDS             complement(36142..36651)
                     /gene="sulA"
                     /locus_tag="ACHKW5_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO7510543.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            36870..37499
                     /gene="sxy"
                     /locus_tag="ACHKW5_001111"
     CDS             36870..37499
                     /gene="sxy"
                     /locus_tag="ACHKW5_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO7510544.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(37462..39624)
                     /gene="yccS"
                     /locus_tag="ACHKW5_001112"
     CDS             complement(37462..39624)
                     /gene="yccS"
                     /locus_tag="ACHKW5_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO7510545.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(39634..40080)
                     /gene="yccF"
                     /locus_tag="ACHKW5_001113"
     CDS             complement(39634..40080)
                     /gene="yccF"
                     /locus_tag="ACHKW5_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO7510546.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            40203..42257
                     /gene="helD"
                     /locus_tag="ACHKW5_001114"
     CDS             40203..42257
                     /gene="helD"
                     /locus_tag="ACHKW5_001114"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO7510547.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(42289..42747)
                     /gene="mgsA"
                     /locus_tag="ACHKW5_001115"
     CDS             complement(42289..42747)
                     /gene="mgsA"
                     /locus_tag="ACHKW5_001115"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO7510548.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(42843..43505)
                     /gene="yccT"
                     /locus_tag="ACHKW5_001116"
     CDS             complement(42843..43505)
                     /gene="yccT"
                     /locus_tag="ACHKW5_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO7510549.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            43678..44091
                     /gene="yccU"
                     /locus_tag="ACHKW5_001117"
     CDS             43678..44091
                     /gene="yccU"
                     /locus_tag="ACHKW5_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO7510550.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(44136..44453)
                     /gene="hspQ"
                     /locus_tag="ACHKW5_001118"
     CDS             complement(44136..44453)
                     /gene="hspQ"
                     /locus_tag="ACHKW5_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO7510551.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(44511..45701)
                     /gene="rlmI"
                     /locus_tag="ACHKW5_001119"
     CDS             complement(44511..45701)
                     /gene="rlmI"
                     /locus_tag="ACHKW5_001119"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO7510552.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEDGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            45796..46074
                     /gene="yccX"
                     /locus_tag="ACHKW5_001120"
     CDS             45796..46074
                     /gene="yccX"
                     /locus_tag="ACHKW5_001120"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO7510553.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLVQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(46071..46400)
                     /gene="tusE"
                     /locus_tag="ACHKW5_001121"
     CDS             complement(46071..46400)
                     /gene="tusE"
                     /locus_tag="ACHKW5_001121"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO7510554.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAKNEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(46491..47150)
                     /gene="yccA"
                     /locus_tag="ACHKW5_001122"
     CDS             complement(46491..47150)
                     /gene="yccA"
                     /locus_tag="ACHKW5_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO7510555.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(47558..48577)
                     /locus_tag="ACHKW5_001123"
     CDS             complement(47558..48577)
                     /locus_tag="ACHKW5_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7510556.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(48555..48797)
                     /locus_tag="ACHKW5_001124"
     CDS             complement(48555..48797)
                     /locus_tag="ACHKW5_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7510557.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(48865..51336)
                     /locus_tag="ACHKW5_001125"
     CDS             complement(48865..51336)
                     /locus_tag="ACHKW5_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7510558.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNA
                     LNDARYQAKYVSAIWQKLLPSQADF"
     gene            complement(51430..51621)
                     /locus_tag="ACHKW5_001126"
     CDS             complement(51430..51621)
                     /locus_tag="ACHKW5_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7510559.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(51618..51806)
                     /gene="dicB"
                     /locus_tag="ACHKW5_001127"
     CDS             complement(51618..51806)
                     /gene="dicB"
                     /locus_tag="ACHKW5_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7510560.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            52380..52565
                     /locus_tag="ACHKW5_001128"
     CDS             52380..52565
                     /locus_tag="ACHKW5_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510561.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(52752..53141)
                     /locus_tag="ACHKW5_001129"
     CDS             complement(52752..53141)
                     /locus_tag="ACHKW5_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510562.1"
                     /translation="MEFKDLLKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(53153..53281)
                     /gene="ydfB"
                     /locus_tag="ACHKW5_001130"
     CDS             complement(53153..53281)
                     /gene="ydfB"
                     /locus_tag="ACHKW5_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO7510563.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(53283..53438)
                     /locus_tag="ACHKW5_001131"
     CDS             complement(53283..53438)
                     /locus_tag="ACHKW5_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7510564.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(53715..54002)
                     /locus_tag="ACHKW5_001132"
     CDS             complement(53715..54002)
                     /locus_tag="ACHKW5_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7510565.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(54002..54193)
                     /locus_tag="ACHKW5_001133"
     CDS             complement(54002..54193)
                     /locus_tag="ACHKW5_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO7510566.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(54221..54622)
                     /locus_tag="ACHKW5_001134"
     CDS             complement(54221..54622)
                     /locus_tag="ACHKW5_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7510567.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            54731..55003
                     /locus_tag="ACHKW5_001135"
     CDS             54731..55003
                     /locus_tag="ACHKW5_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7510568.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            54987..55412
                     /locus_tag="ACHKW5_001136"
     CDS             54987..55412
                     /locus_tag="ACHKW5_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7510569.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(55619..56074)
                     /locus_tag="ACHKW5_001137"
     CDS             complement(55619..56074)
                     /locus_tag="ACHKW5_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510570.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            56153..>57109
                     /locus_tag="ACHKW5_001138"
     CDS             56153..>57109
                     /locus_tag="ACHKW5_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7510571.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCTGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGK"
BASE COUNT        14511 a        13783 c        14556 g        14259 t
ORIGIN      
        1 tactgataag cgtagcgcat caggcaatgt tgcgtttgtc atcagtctca gcccgctttt
       61 cagcgggctt cattgttttt aatgcttaca gcactgccac aatcgcttcg cacagcggag
      121 ccatgttatc tggtgtcatc ccggccacat ttacgcgacc agaagcaacc gcatatacgc
      181 caaactcttc gcgcagacgc agcacttgtt cttttgtcag gccactgaag gagaacatgc
      241 cgttctgttt gatgataaag ctgaagtcgc ggtttgcgcc tttttcctgc agcgtattga
      301 cgaacaactg acgcatacgc tgaatacgct ggcgcatatc agtcagctct tgttcccaaa
      361 tcgcacgtaa cgcatcgttg ctcaggatgg tggcaacaac agaagcgccg tgtgctggtg
      421 ggttagagta gttagcgcga atcgccgctt tcatttggct gaatgcgcga tcaacggttt
      481 cactgtcggc agcaaccaga gtacaagcgc caacacgctc gttgtacagg ccaaagtttt
      541 tagagtagga actggcaaca atcagctctt tatgcatagc cgcgaaagcg cgcagtcctt
      601 cagcatcttc ttccagacca cgggcaaaac cctggtaagc gaagtcaaac agcggtaacc
      661 agcctttctc aacggagagt tgtgccagtg tctgccattg ttccagcgta gggtcgatac
      721 cggttgggtt atggcagcag ccatggaaca gcactacgtc gccagcctga gcttcattca
      781 ggctgttaat cagtgcatcg aagtcaagag tgtgattttc cgcatcataa taagcgtatt
      841 cacgaacttc cagacctgca gagttaaaga cgctcttatg gttcggccag cttgggttgc
      901 tcacccacac tcgcttaacg ctggtatttt ttgccaggaa atcggcagct acgcgtagtg
      961 cgccagtgcc ccccggagtc tgtgccgtgc gagcacgttt gtcattgatc agggcgctac
     1021 ctttaccaaa cagcagttcc tgagtgcagc gaccaaattc agggatgccg tcaatgccga
     1081 ggtaattttt ggtggtttca ttttcgagca gatactgttc agcctttttc acgctggtca
     1141 gtaccggggt tttgcccgtc tcatctttat agacaccaat cccgaggtta attttgccgg
     1201 gacgttcatc ggcacgaaac agatcggcca ggcccagaat cgggtcggca ggagcggcgg
     1261 taatgttctc aaacatgacg aggttccatt atggttacag aagggaagtc cgctatcagg
     1321 gtaacgggag atttacaaaa ttccaactat tactgatgaa aacgcaggct gtttttgcaa
     1381 gacgtgagat tgctctggaa ggtataaaaa aaacaggacc aaagtcctgt tttttcggca
     1441 tttaacaaag aggtgtgcta ttagaactgg taaacgatac ccacagcaac ggtgtcgtct
     1501 gaacctacgc ccagtttgtt gtcagaatcg atctggttga tgatgtagtc aacataggtg
     1561 gacatgtttt tgttgaagta gtaggttgcg cccacttcaa agtagttcac cagatcaaca
     1621 tcaccgatac cttctacgtc tttcgcttta gatttggtgt aagcgatgga cggacgcaga
     1681 ccgaaatcga actggtattg cgcaactaac agaacgtctt gcgttttgtt ggcgaagccg
     1741 ctggtgtttg taaatttatt agtgatcggc gtagcgttac gggtttcacc gtagttcgct
     1801 gccaggtaga tgttgttcgc gtcgtacttc agaccagtag cccactgttc agctttttta
     1861 ccgttgccaa gaggttgagc ttcttgcagg ttggtacggt cagctgcacc ataagcacca
     1921 acgataccaa agccttcgta ttcgtagctg atagaaccgc caacaccgtc gccgttagaa
     1981 cggcgtgcag tgtcacgctc gtttttaccc aggtactgaa cagcgaagtt caggccatca
     2041 accagaccaa agaagttgga gttacgatag gtagcaacgc cgccaacacg accaacgaag
     2101 aagtcatcgc tgtatgcagt atcaccacca aattctggca gcatatcggt gtaacccagt
     2161 gcatcataaa ccacaccgta gttacggccg taatcgaaag aaccaacgtc agcgtattta
     2221 agacccgcga atgccagacg cgttttgtta ccagtttgag cgtcagcgcc ttcagagttg
     2281 ttaccctgga agttatattc ccactgacca taaccggtca gatcggaatt gatttgagtt
     2341 tcccctttaa aaccaagacg ggcataggtc atgtcgccat tgccaccgta actgttttca
     2401 ccgttaccct tggaaaaata atgcagacca acagctttac cgtacagatc tactttgttg
     2461 ccatctttgt tatagatttc tgcagcgttt gcagtacctg ctactaacag agcagggacg
     2521 atcactgcca gaatattgcg cttcatcatt atttattacc ctcatggttt tttttatgac
     2581 acctgccact gccgtcaata agttctgtca ataaaaattt acggaactat tgatgagagt
     2641 ttggtgtctt tatgtgtctg caggcatctt tccattcaaa ctaacgtttc gctaccgtga
     2701 aagtgctaca aagataaaga tttagtttca aaaagaaaaa atatgtaacc aaaagtaaaa
     2761 attaaggaac tttgtgaaca ccgtcatatt tccatagaaa cgtgatgata tttacagcaa
     2821 tttttatcta tttatatgat ttacttatat ttaaattaac taaacggaaa ttttgtttct
     2881 gatggaaact ttatcgacct ggcacaaaat cctctttcag acatccagaa tgccaaaaaa
     2941 tagtatgaaa attgtgctat cagctaagaa aaaactaatc cgcaataaga atgcgggtta
     3001 ttttttgtgc gtaaatgttg caatgtaata ttccgtaaca ggatgatcgt tatggcgggg
     3061 ttgcaaatag attgcttgac ttaacaaaca gataacttga cagaaaggat aaaaaagagc
     3121 cagcaggcgc tggctctttg aggaagaaaa tcagaagctg gcgttgcgcg gagtacgtgg
     3181 gaacggaatc acatcacgca cgttttgcac gccagttacg taagcaatca ggcgttcaaa
     3241 accaagaccg aaacctgaat gcggaacagt accgtagcga cgcagatcgc gataccacca
     3301 gtaatcttct ttattcaggc ccatttccag catacgctcg tccagaacgt ccagacgttc
     3361 ttcacgctgg gagccaccaa tgatctcacc gatgcccgga gccagaacgt ccatagccgc
     3421 aacggtttta ccgtcttcgt tgaggcgcat atagaacgct ttaatatctt tcggatagtt
     3481 tttaaccact accggtgctt taaagtgttc ttccgccaga taacgctcat gctcagaaga
     3541 gagatcgacg ccccagtaaa ccgggttttc aaacttcctg ccgcagtttt cgagaatggt
     3601 cactgcgtcg gtgtaatcca cctgcgcgaa atcggcctca atgaagcgtt ccagacgtga
     3661 aacggcatct ttatctacgc gttcagcgaa gaatttcatg tcgtctgcgc gttcttcgag
     3721 aaccgctttg aagacatatt tcagcatggc ttcagccaga cccgcgatat cgttcaggtt
     3781 agcaaacgcc acttccggct ccagcatcca gaattccgcc aggtgacggc tggtgttgga
     3841 gttttcagca cggaaagtcg ggccgaaggt ataaattttg gataatgcgc aagcgtaggt
     3901 ttcgccgttc aactggccag atacggtcag gaaagactct ttaccaaaga agtctttgtc
     3961 gaaatccact ttgccctgat cgttacgcgg caggttttcc agatccagcg tagaaacgcg
     4021 gaacatttcg cctgcacctt cggtatcaga tgcggtaatc agtggcgttg aaacccagaa
     4081 gaatccctgc tcgttaaaga agcgatgcag cgcctgcgcc agcgtatggc gaacgcgcgc
     4141 gacggcacca atcaggtttg tgcgcggacg caggtgagcg acttcacgca gatactcaat
     4201 gctgtggcgt tttgccgcca tcgggtaagt gtctggatct tcaacccaac cagcaacttc
     4261 aactttgctg gcctgaattt caaattgttg cccctggccc ggcgacgcca cgactttacc
     4321 cgtcacaatg accgagcagc cggtggtcag acgcaggacg tcttcattgt aattgggcag
     4381 agaattattg atgacagcct gtacaggatc aaagcaggaa ccgtcataaa cggcgaggaa
     4441 ggagatgcca gcttttgaat ctcggcgggt acgtacccat ccgcgtacgg tgacttcgct
     4501 gtcaacggct acgcggccct ggagtacgtc ggctacaggc acaacgctca taatattctc
     4561 tctgttaata gtcggaaaaa ataaacactt gtccacccgt aaatgggggg atacctatgt
     4621 tacctggcat ctgcaatcag acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt
     4681 aaaggtaaaa aaagggagcc gattagctcc ctgatgatat taactggctt ttttaatatg
     4741 cggaaggtcg aacgctttgc gcagcgcccg aacaaacgct ttatcgtggc agatagtttt
     4801 gccagggcag tcagaaagtt tcgccaccgg tttaccgtta cactcaacca acttaatcac
     4861 aatattcagg ggttttacct gggggatatc gcaggtcagg cgagtcccaa taccaaaact
     4921 taattgcacg cgggaagaga agtggcggta tagctcaacc gctttgcgta aatccagatt
     4981 gtcagagaaa accagcgttt tactctgtgg atcaattccc agcttttcat aatgtacaat
     5041 ggctttttca ccccattcaa ccgggtcgcc agagtcatga cgcaggccct gatagcgact
     5101 ggcgaactcg acaccgaaat cacgcaggaa agcatccata gtgatgcagt cggttaatgc
     5161 aatgccaagt tggtcgggat attcttccag ccaggcagca agtgcagctc gctggctgtt
     5221 ggctagatcc gggctgattt gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat
     5281 cggcgtgagg gaaagccgac gcgccagatc gtagttgctg gtgcccacga accaggattc
     5341 ctgttgcaga cgcttaacga tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc
     5401 aaaatccata agatggaagc gcgacatatc aaggccggcg gttaacgccg agaagtcgac
     5461 taatttgctt tccagcgtgt cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg
     5521 atgtaccatt tcactgataa ccgccagcaa aggaacttcc cagaggatga cttcacgcca
     5581 cgggccgctt aaacgaatat ccagcttgcc attatcgttg gatacggtga cttgttccgg
     5641 gttaaagcgg aactcgcgta accagttaag atagtcggcc ttaaagaaag gcagagcaga
     5701 gagccactga tattcatcat cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg
     5761 aatagcatcg gcataaatac ccagcagatc gtcacctcgg caacgaaact ccgccgcgac
     5821 atgcacatcg taatagtgat gaaacacggc ttgctgcata tgcaacttat aagcatctgt
     5881 atccagcaac gagtgcagaa caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc
     5941 tcgtacagga gcgtttagta caataaacat cttcaggaac tgctggagta taccttgttt
     6001 cgcaatttat tgaaccccga tcacaccata tgccaccttt ctggtcgatg gcattacgcg
     6061 gtgtatgtta taaaaatgta gcaataaagg cgtttgtacc tgaaaagatg aagattctgc
     6121 atagcgcgat ttacgcaaca ggaatagact gaacaccaga ctctataaaa gatgctaaag
     6181 gttatttatg actcaacagc cacaagccaa ataccgtcac gattatcgtg cgccggatta
     6241 ccagattact gatattgact tgacctttga cctcgacgcg caaaagacgg tcgttaccgc
     6301 ggtcagccag gctgtccgtc atggtgcatc agatgctccg cttcgtctca acggcgaaga
     6361 cctcaaactg gtttctgttc atattaatga tgagccgtgg accgcctgga aagaagaaga
     6421 gggcgcactg gtcatcagta atttgccgga gcgttttacg cttaagatca ttaatgaaat
     6481 aagcccggcg gcgaatacgg cgctggaagg gctttatcag tcaggcgatg cgctttgcac
     6541 ccagtgtgaa gccgaaggtt tccgccatat tacgtattat ctcgaccgcc cggacgtgct
     6601 ggcgcgtttt accaccaaaa ttattgccga taaaatcaaa tatcccttcc tgctttccaa
     6661 tggtaaccgc gttgcgcaag gcgaactgga aaacggacgc cattgggtac agtggcagga
     6721 cccgttcccg aaaccgtgct acctgtttgc gctggtggca ggcgactttg atgtactgcg
     6781 cgataccttt accacgcgtt ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg
     6841 caaccttgat cgcgcgccgt gggcgatgac ctcgctgaaa aactccatga aatgggatga
     6901 agaacgcttt ggcctggagt atgacctcga catctatatg atcgtcgcgg tggatttctt
     6961 caatatgggc gcaatggaga ataagggtct gaatatcttt aactccaaat atgtgttggc
     7021 ccgcaccgac accgccaccg acaaagatta tctcgatatt gaacgcgtta tcggccatga
     7081 atatttccat aactggaccg gtaaccgagt gacctgccgc gactggttcc agctcagcct
     7141 gaaagaaggt ttaaccgtct tccgcgatca ggaattcagc tctgaccttg gttcccgcgc
     7201 ggtaaaccgt atcaataatg tgcgcaccat gcgcggattg cagtttgcgg aagacgccag
     7261 cccgatggcg cacccgatcc gcccggatat ggtcattgag atgaacaact tctacaccct
     7321 gaccgtttac gagaagggcg cggaagtgat tcgcatgatc cacaccctgc tgggcgaaga
     7381 aaacttccag aaagggatgc aactatattt cgagcgccat gatggcagcg cggcaacctg
     7441 cgacgacttt gtgcaggcga tggaagatgc gtcaaatgtc gatctctccc atttccgccg
     7501 ttggtacagc cagtccggca cgccgattgt gaccgtcaaa gacgactaca atccggaaac
     7561 cgagcagtac accctgacca tcagccagcg cacgccagcc acgccggatc aggcagaaaa
     7621 acagccgctg catattccat ttgccatcga attgtatgac aacgaaggca aagtgatccc
     7681 gttgcagaaa ggcggtcatc cggtgaattc cgtgctgaac gtcacccagg cggaacagac
     7741 ctttgttttt gataatgtct acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc
     7801 gccagtgaaa ctggaatata agtggagcga tcagcaactg accttcctga tgcgtcatgc
     7861 gcgtaatgat ttctcccgtt gggatgcggc gcaaagtttg ctggcaacct acatcaagct
     7921 gaacgtcgcc cgtcatcagc aagggcagcc gctgtctctg ccggtacatg tggctgacgc
     7981 tttccgcgcg gtgctgctcg atgagaagat tgatccggcg ctggcggcag aaatcctgac
     8041 gctgccttct gtcaatgaaa tggctgaact gttcgatatc atcgacccga ttgctattgc
     8101 cgaagtacgc gaagcactca ctcgtactct ggcgactgaa ctggcggatg agctgctggc
     8161 tatttacaac gcgaattacc agagcgagta ccgtgttgag catgaagata ttgcaaaacg
     8221 cactctgcgt aatgcctgcc tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt
     8281 gctggtgagc aagcaatacc atgaagcaaa caatatgacc gatgcgctgg cagcgctttc
     8341 tgcggcggtt gctgcacagc tgccttgccg tgacgcgctg atgcaggagt acgacgataa
     8401 gtggcatcag gacggtctgg tgatggataa atggtttatc ctgcaagcca ccagcccggc
     8461 ggcgaatgtg ctggagacgg tgcgtggtct gttgcagcat cgctcattta ccatgagcaa
     8521 cccgaaccgt attcgttcgt tgattggtgc gtttgcgggc agcaacccgg cagcgttcca
     8581 tgccgaagat ggcagcggtt accagttcct ggtggaaatg cttaccgacc tcaacagccg
     8641 taacccgcag gtagcttcac gtctgattga accgctgatt cgcctgaaac gttatgatgc
     8701 caaacgtcag gagaaaatgc gcgcggcgct ggaacagttg aaagggctgg aaaatctctc
     8761 tggcgatctg tacgagaaga tcaccaaagc actggcttga tacttttgag ccaaaatgcc
     8821 tgatgcgacg cttgacgcgt cttatcaggc ctacaactcc gttgaaattg ctggttttgt
     8881 aggctggata aggcgttcac gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc
     8941 ttgacgtgtc ttatcaggcc tactagtccg ttgaaaatgc tggttttgtg cgccacatcc
     9001 ggcatattaa ccatgacgcc gaatcagttg ttcagaatgc ccacgccgca ttacccgttg
     9061 caatacttcc gcttccagct ccgctaatcg caccgaccct aatcgacgcg gacgggggat
     9121 atccactgtc aaatccagac caatttttcc ctcttcaatt aacaacaccc ggtcagccat
     9181 tgctaccgct tcgctcacat catgcgtcac caacagtacg gtaaagccgt gctgctgcca
     9241 aagtgacaca atcaaatcct gcatctcgag tcgcgttaag gcgtccagcg ccccgagcgg
     9301 ttcatcaagc aacaataaac ccggtcgatg aatcaacgct cttgccagcg ccacacgctg
     9361 tttctgcccg ccagaaagtg cggcaggcca ttccccggcg cgattctcca gtcctactgc
     9421 agccaacgcc cgacgcgcgg catcgcgcca ctggccttta aggcctaacc caacgttatc
     9481 aatcaccgat ttccacggca gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg
     9541 aatctcagcc agcggtgtgg tgcccgctaa cacatcgcct gcggttggcg tttccagacc
     9601 tgccagcagg cgcagcaggg tacttttgcc accaccgctg cggcccacca ccgccacaaa
     9661 ctgacctgcc ggaatatgta aatccagttg gttcaggacg atattttccg cgtaatgttt
     9721 gcttactgca ttgagcaaca atggtgtgcc ctggttcaga cgagcagtat tcataccgtg
     9781 gcctccttca aatgataagc cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg
     9841 ctgacgtcag caagtttgcc gagcagggcg taaagaataa tagcgactac caccacgtcc
     9901 gtttgcaaaa actcccgcgc attcatcgcc agataaccaa tgcctgaatt ggcagaaatg
     9961 gtttcggcaa caatcagcgt cagccacatc aggcccaacg caaaacgcac gccgaccata
    10021 attgagggca gggcaccagg caggatcaca tggataaaca gcggtatgcc ggataatcca
    10081 tagctacgcg ccatttccac cagaccgcga tcgatattac ggatgccatg ccaggtgttg
    10141 atataaatgg ggaatagcgt gccgagcgcc accagaaaga tttttgcgga ttcatcaatg
    10201 ccaaaccaca aaatcaccag cgggatcagc gccagatgcg gtacgttgcg caacatctga
    10261 attgaggtat ccagcagccg ttctccccag cgagatagcc cgctaatcag tcccaaaatc
    10321 aaccccagcg atccgccaat tgaaaagcca atcagcgccc gccaggaact gatcgccaga
    10381 tgctgccaca gttcgccgct ggcggagagc gtccagaacg ccgtcaccac cccttccggt
    10441 gagggcaaaa tacgcgtcga cagccagcca actgaggagg ccagttgcca caccgccacg
    10501 atgcccaccg gtaaaaacca gggggcaacg cgcaataacc acttcttcac tggcgttgcc
    10561 attatttctt ccttagcttt gcgcgacttt acgggggata aaatcattcg ccaccgcttc
    10621 gccttgcgga ttcagcggct gcggctgggg aatttccgga atggcgacat ccagatgtgg
    10681 gaacagcaac tcgccgaccc gatacgcttc ttccagatgc ggatagcccg aaagcacaaa
    10741 gctgtcgatg ccaagcgcgg cgtattcgtt gattcgcgca gcgaccgtag gaccatcgcc
    10801 caccagcgcc gttccggcac cgccgcgcac taagccaacg cccgcccata aattggggct
    10861 gatctccaga ttgtcgcgct tgccgttatg taacgccgcc attcgctgtt gtcctacgga
    10921 atccgtccgg gcgaatgcgg cctgtgcttt ggcgatagtt tcatcatcaa gatgcgagat
    10981 taaccgctcg gcggcctgcc acgcttcgtc gttagtttca cgaacaatca catgcagacg
    11041 aataccgaaa cgaattttgc gtccatgcgc ggcagctttc gcccgcactt gttcgatctt
    11101 ctcttttact agttctggcg gttcgcccca ggtgaggtag agatcaactt gttctgccgc
    11161 cagctcctgg gcgacgtctg acgatccgcc aaagtaaagc ggaggataag gctgttgaat
    11221 cggcgggaag agcagttttg ctccgcgcac atgaatatgt ttaccgttga aattgacggt
    11281 ttcccccagc aataagcgcc gccagacctg ggtaaattcc gccgaggctt cgtagcgctc
    11341 gctatgatca aggaacacgc cgtcgcctgc cagctcttgt ggatcgctgc ctgtgaccag
    11401 attaaacaac gcacgaccat ttgagagacg gtcaagcgtg gcggcctggc gagcggcaac
    11461 ggtaggtgag gttacgctgg ggcgcagggc gacaagaaac ttcagccgct gcgtcaccgg
    11521 gatcatcgac gccgccacca gccacgcatc ttcacacgat cgccccgttg ggatcagcac
    11581 accggtatag cccagacgat ccgccgcttg cgcaatttgt tgcagatagc cgtgatcaac
    11641 cgggcgtgaa ccttcttccg ttccaagata atgcccgtca ccatgggttg gtaaaaacca
    11701 gaacatattc agactcataa ttgttttcct tccagttgag tgggctgcca gatgcgctgg
    11761 cgaatatcga cttttttcgg caccagacga ttttcataaa acagatctgc cgtttgctgc
    11821 tgtaaggcgg caacctcggc gttaaccggt ttgatggtgg taggagggcg atgatctaag
    11881 taagaggcaa tcaccggtgc cggtaagccc atcgtttttg ccagtaaagc gatgctttgc
    11941 tcgcgctggc tgcgggttaa cgcatcggcc tcactaaagg ttgcgagtac gccctgaata
    12001 aaagcgccgt ttttttctgc atagggacga gctgccagat aaaacgatcc agtttgattg
    12061 agatcggtgc cgtctttcag cacccgcacg ccgccctgta ataatgcagc agagtagtag
    12121 ggatcccaga tagcccaggc gtcaacgtta ccttgctgga acgcggcgcg ggtatcagcg
    12181 ggcgtcaggt aagtgggttg gatatcggta aacttaagtc cggcctgacg cagtgcacgc
    12241 agtaaaaggt tgtgtgaact ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg
    12301 gctacggttt tgatcgggct gttttctgcc accagaatca cttcagcttt gggcttcggt
    12361 ggctcgacgc ccacatatac caaatcagcc ccggcagcct gggcaaagat tggcggaata
    12421 tccccggtac tgccgagatc aatactgcca acgtttaacg cttccaacat ttgcggaccc
    12481 gcggggaact ccacccagga gatttttgat tgcggatagc gtttttccag taactggtgg
    12541 ctttttgcca gtaccatacc aatactgcct ttctgatagc ctatacgtaa cgcttcaggc
    12601 gaggattctg cagcaaccgc aatcgtagag acgctaagca atcccaccag cgccagttta
    12661 atgatgttac gcatgggcat tacctcgcag agacagaagg tcaggaacct gaacatcgcg
    12721 gcggtgcaat gcctgccaga aagtttctag cgcggtatca agacgggttt gcagatttgg
    12781 cgtgaactgg ggtctgtgat ggtaatcaat tacttgtgag tcatcggcaa acacgccgtg
    12841 caggatctcc tgagctttca gtgcgcttaa aactggttta agggcataat cgaccgccag
    12901 cagatgggcc acggtaccgc ccgtcgccag cggtagcacc actttgcctt gcaaagcgcg
    12961 ctctggcagc aggtcgagca gggttttcag cgcgccggaa taggcggctt tatacacagg
    13021 tgtagcgact atcagtccat cagcctgttg cagctgttcg gtgaaggtct tgagtgccgg
    13081 actatcgaaa cgagcataaa gcagatcttc cggggcgaag ttttgcagat tccagtgata
    13141 aacctctaca tcgaggccat ttagtttttc ccgcgcatat tccagcaagg agctggagcg
    13201 agaaggaaag cgaggactac ccgccagggt gatgacacgc atactctctc cttataacca
    13261 attgttcttt ttttgttaac attgataaca attcggtcag tctgtcggag agacaagaaa
    13321 atgccaaata taaattttgt gtatcttttt ctgaaaataa acaaaagaaa gggctttata
    13381 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
    13441 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
    13501 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
    13561 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
    13621 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
    13681 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
    13741 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
    13801 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
    13861 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
    13921 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
    13981 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
    14041 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
    14101 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
    14161 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
    14221 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
    14281 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
    14341 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
    14401 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
    14461 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
    14521 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
    14581 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
    14641 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
    14701 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
    14761 tgacagacct gaaaataggt gggaaagtag ttaaaaatca aatgatttcc ccctttgata
    14821 aataccaatt ttctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
    14881 atgactacgg ggcggaaacg ccgcaactca actgtaaatc gtaagccgtc ttcagttaag
    14941 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
    15001 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
    15061 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
    15121 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
    15181 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
    15241 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
    15301 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
    15361 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
    15421 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
    15481 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
    15541 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
    15601 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
    15661 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
    15721 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
    15781 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
    15841 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
    15901 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
    15961 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
    16021 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
    16081 aggagagcaa atttgcccag gccacgttgc agtggggcgg accgtgggga acgacatggt
    16141 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
    16201 ttggcgattt cggagcaatt tcgttcgatg tgacccaggc gaagagtacg ctggcagacc
    16261 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
    16321 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
    16381 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
    16441 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
    16501 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
    16561 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
    16621 tctcgctggg ggtttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
    16681 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
    16741 ataccaggaa aaaaaattac gcctggatga cctctaacac cagtatcgat aacgaagggc
    16801 acattacaca aaacctgggt ttaacggaga cactactcga tgacggtaac ctgagctaca
    16861 gcgtgcaaca gggatataac agcgagggga aaacggctaa tggtagcgcc agcatggact
    16921 acaaaggggc gtttgcagat gcccgagtgg gctacaacta cagcgataac ggcagtcaac
    16981 aacaactgaa ctacgctctt tcaggcagtt tagttgccca ttcacagggc attaccctgg
    17041 ggcaatcgct gggggaaact aacgtcctga ttgcagcacc aggcgcagag aatactcgtg
    17101 tggcgaacag caccgggctg aaaactgact ggcgcggata taccgttgtc ccctatgcca
    17161 cttcttatcg ggaaaatcga atcgcacttg atgcggcatc gttaaaacgt aacgtggatc
    17221 ttgaaaatgc agtagtcaac gtggttccca ccaaaggggc gttggttctg gcggagttca
    17281 atgcccatgc gggagcaagg gtattaatga aaacatcaaa gcagggtata ccgctgcgtt
    17341 ttggcgcgat agcgacgctg gacggcatac agactaatag cggcattatt gatgatgatg
    17401 gctcgctcta tatgtccggt ttgcccgctc agggagcgat aactgtacgc tggggcgaag
    17461 ctcccgatca aatttgtcat atcagttacc agcttaccga acaacaaatt aactctgcga
    17521 ttacgcgaat ggatgccata tgcagataat ctttggagaa aaatgcgtgt tattactacg
    17581 actatttttt gccgccgtct taatgctatg gtgcgctcaa actgctgctt atagcgggca
    17641 gtgtcatacc actcagggga atccgtatat tggcgtcaat tttggcgtta aaaccctgga
    17701 ggaagaagaa aatacggctg gagtagttaa agacaaattt tatcagtgga acgaatcgaa
    17761 tgattattat gtttcctgtg attgcgataa agacaatgtc agaagtggcc gatgggcatt
    17821 cgccgcggat tcaccgttag tctatttagg cgacaactgg tacaaaatta atgactatct
    17881 tgccgccaaa gttttattgc aggttaaagg cagttctcct actgcggttc ctttcgaaaa
    17941 cgtgggcaca ggggcagata cacgatggca tatttgcgat cccggcggtc aacgtttagg
    18001 tggccagggg gctagcggta atagcggtag cttttccctg aaaatattgc agccgttcgt
    18061 tggttcggtc gtcattcctc ctatggcgct ggcgcgatta tttgaatgct acaacatacc
    18121 cgcaggtgat tcctgcacga ctacaggtac accggtttta gtgtattacc tgtctggtac
    18181 tatcaattca cttggctcat gttccgtcaa tgccggagaa acaatcgagg tcgatctggg
    18241 cgacgtattt gcggctaact ttcgtgttgt agggcataag cctcttgggg ccagaacggc
    18301 agaacttgca attccagtca ggtgtaacac gggaaacgcg gggttagtta acgtcaacct
    18361 gagtctgacg gcaaccacag accccagcta tccccaggcg attaagacgt cacgtcctgg
    18421 cgtgggcgtg gtggtgaccg atagccagaa caacattatt tcccctgctg gtggaacatt
    18481 accgctctct attcctgatg atgcagacag tatcgcgcga atgaatgtct atccagtcag
    18541 cacgacaggt gtaccaccag aaaccgggcg atttgaagcc acggcaacgg tgagaataaa
    18601 ttttgattaa cgcgtgaacg tatgaagaaa aaaacgatat ttcagtgcgt tatcttgttc
    18661 tttagcattc ttaacatcca tgtcgggatg gctgggcctg aacaagttag tatgcatatt
    18721 tatgggaatg tggtcgatca gggctgtgat gtcgccacca aaagtgcatt acaaaatatt
    18781 catattggtg attttaatat cagtgatttt caggccgcga ataccgtaag cactgctgct
    18841 gatttgaata tcgatatcac cggttgtgcc gctggtatta ctggcgcgga cgtccttttt
    18901 agcggcgagg ctgacaccct tgcgccgaca ctgctcaaac taactgacac aggcggaagc
    18961 ggtggtatgg caacggggat tgccgtgcaa attcttgatg cgcaaagtca gcaagaaatc
    19021 ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac actcaaatat
    19081 caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac ggcggttctc
    19141 tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct ggattctgtt
    19201 cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacag tcaacattag
    19261 cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa tcaatgtgca
    19321 gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg agaaatcttt
    19381 catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgacgtca ccttttcagg
    19441 aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg atgcagccag
    19501 cgggctggca attgcgattc tggatgatgc gaagatattg attccgctta accaggcgag
    19561 taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc aattgcgacc
    19621 tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg tacttcatta
    19681 tgactaatac gtggaacaga ttggcgctct tgattttcgc cgttttatcg ctgctggtgg
    19741 cgggtgaatt gcgggcagga gtggtggtcg gcggaacgcg atttatcttt ccggcagaca
    19801 gagaatcgat atctatttta ctgactaata ccagtcagga atcctggctt attaacagta
    19861 aaatcaaccg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca gcaccattac
    19921 tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg cgcttgctga
    19981 gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta agcattgcca
    20041 gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc tcggtattta
    20101 aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat caacaattac
    20161 gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac tacattaacc
    20221 tgattcagtt gagcgtaaat ggtaaagcgt taagtaatgc gggagttgtg ccgcctaaaa
    20281 gccagcgtca gacaagctgg tgtcaggcaa tcgcaccgtg tcatgtcgcc tggcgggcga
    20341 taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga aacaggttcg
    20401 gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa ctcgcacata
    20461 atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc ccttcgttcg
    20521 taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc agcaattacg
    20581 ccgtattaca ggaacgccgt ttgaagcact ggtgcggcag aaagtgcctg cgaaacctgt
    20641 taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc ttgataaaga
    20701 cggggagtgc attgacgcgt taggcgcgat gggatttgga tcgatcgaga tcggtaccgt
    20761 cacgccacgt ccacagccag gtaatgacaa gccgcgtctc tttcgtctgg tagatgccga
    20821 aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg tagagaacgt
    20881 aaaaaaggcc cattatgacg gcgtcctggg tattaacatc ggcaaaaata aagatacgcc
    20941 agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg cctatgcggg
    21001 atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc tgcaatatgg
    21061 tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt tgcaagcgat
    21121 gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg aagaagaatt
    21181 gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg caaccaatac
    21241 cacactcgat cgttctcttg ttcagggaat gaaaaattgc gatcaaaccg gtggcttaag
    21301 tggtcgtccg cttcagttaa aaagcaccga aattattcgc cgcttgtcac tggaattaaa
    21361 cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg cgcgtgaaaa
    21421 gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta aaggtccgcc
    21481 gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc tttataagat
    21541 aatcagggct ttattttcag ccctggttgt tttatattca tcgctgttgc ttatttagac
    21601 attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt gttgctgcgg
    21661 ttttataggg taggggagag gcagatgcga attaaaccag acgataactg gcgttggtat
    21721 tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct atttcgctca
    21781 cgttttgcgc gcaagatgtt gactccagat gccttttctc ccgcaggctt ttgcgttgac
    21841 gacgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt atctaaagaa
    21901 caaaaagccg aactggtgct gaatgcactg gtagcaattc gctatctcaa accgcaaatg
    21961 ccgaaaagct ggcattttgt ttcccatggt gaaatgtggg tgcccatgcc gggcgatgcg
    22021 gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt tgaatctggc
    22081 gaaaatgccg cactatgcct gctggcacaa ccctgcgttg ttattgcggg tcgcgcgatg
    22141 cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt taatgttgac
    22201 agcttcagcc tcgaacaggc agtctaacgc gccaacttaa gtgcagtctt cggtacacag
    22261 ctacagcaaa gaatggtgcc atcatcgccc attgctgatt tttttagcgg cgtgacttcg
    22321 ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga ataagggata
    22381 cgaattccct gattttctaa ttgctccagc aacacctgtt ggttatttcc acgaaatgcc
    22441 tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt ggcggtatca
    22501 tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc cacctcatcg
    22561 ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc gacatcgccg
    22621 ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc cggatgtttt
    22681 tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt aaccacatca
    22741 aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc tgacgcgccg
    22801 gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca acgttgttgg
    22861 agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc aaatgacagg
    22921 ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca gcgtaattgc
    22981 acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc aattcgcgcg
    23041 gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc agcaaaacga
    23101 acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac aggtgaagga
    23161 gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc aggttccgtg
    23221 atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg tgtaagacca
    23281 atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcac cacggtgcgg
    23341 tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag ctataatgcg
    23401 caacaatttt cttagcgcct gtcccattag gctattttat ttgtcagttt ggccctgggt
    23461 tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctg tccatgtcca
    23521 gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt gacgatatga
    23581 attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact gaactggaaa
    23641 acctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag ggcgacacac
    23701 ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg ttgccgctgg
    23761 gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg atcaactgga
    23821 cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg aatgacacca
    23881 tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat gctttcacgc
    23941 ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc gttaacgtct
    24001 ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt ttacatctgc
    24061 gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca gccgctattg
    24121 tgatgcgatc cggctggcag ccaggaacac cgctgctcga tccgatgtgt ggttccggta
    24181 cgttgctgat tgaagcggcg atgctagcga ccgatcgcgc accaggcttg caccgtgggc
    24241 gttggggctt tagcggctgg gtgcagcatg atgaagatat ctggcaggaa gtgaaagcgg
    24301 aagcgcaaac tcgtgcccgt aaaggcctgg ctgagtatag ctctcatttt tacggttcgg
    24361 acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg gggattggtg
    24421 aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg ccgaaagggc
    24481 cgtacggcac agtgttgagc aacccgccat acggggaacg tctggacagc gaaccggcgc
    24541 tgattgcgct gcatagcctg ctgggtcgga tcatgaaaaa ccagtttggt ggctggaatc
    24601 tctctttgtt tagtgcctcg ccggatctgc taagctgctt gcagctgcgt gcagacaaac
    24661 agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac gttgccgaaa
    24721 gcactccaga cagcaaaccg gcgatggtag cggaagacta cgccaaccgc ctgcgtaaga
    24781 acctcaaaaa attcgagaag tgggctcgcc aggaaggaat tgaatgttac cgcctgtatg
    24841 acgccgatct accggaatat aacgttgccg ttgaccgtta tgccgactgg gtggtggtgc
    24901 aggagtatgc gccgccaaaa actattgatg ctcacaaagc gcgtcagcgt ctgttcgata
    24961 ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg ctgaaaaccc
    25021 gtgaacgcca gaagggcaaa aatcagtacc agaaactggg cgagaagggc gagtttcttg
    25081 aagttaccga atataacgct cacttgttgg tgaacctgac ggattacctc gataccggtc
    25141 tgtttctcga tcaccgtatc gcccgtcgta tgctcggtca gatgagcaaa ggcaaagatt
    25201 tcctcaacct gttctcttat accggcagcg ccaccgtgca tgcgggatta ggcggtgcac
    25261 gcagcaccac caccgtggat atgtcgcgta cttatctgga gtgggcagaa cgcaacctgc
    25321 gtttgaatgg cctgaccggg cgtgcgcatc gcctgattca ggccgattgc ctggcgtggc
    25381 tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc ttctctaact
    25441 caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg atgaaagatt
    25501 tgaaacgtct gctgcgtgca ggtgggacga ttatgttctc gaacaataaa cgtggcttcc
    25561 gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt acgcaaaaaa
    25621 cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg attaccgcag
    25681 cctaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    25741 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    25801 ggccgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    25861 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    25921 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    25981 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    26041 aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct gtggcagctg
    26101 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaacgt tgcgctgtcg
    26161 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    26221 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    26281 gaagggtttt tgaaaacttt caacgggacg attattttca tctcccacga ccgttcgttt
    26341 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    26401 gggaattacg accagtacct gctggaaaaa gaagaagccc tgcgcgtgga agaattacaa
    26461 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    26521 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcgatgcg tcgcgagcgt
    26581 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    26641 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    26701 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    26761 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    26821 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    26881 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    26941 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    27001 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    27061 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    27121 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    27181 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    27241 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtctgtggcg
    27301 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    27361 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    27421 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    27481 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    27541 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    27601 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    27661 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    27721 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    27781 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    27841 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc ctatgcgttg
    27901 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    27961 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    28021 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    28081 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    28141 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    28201 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    28261 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    28321 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    28381 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    28441 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    28501 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    28561 gtggcggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    28621 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    28681 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    28741 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    28801 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    28861 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    28921 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    28981 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    29041 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    29101 ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    29161 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    29221 ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta tggaaaaatt
    29281 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    29341 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    29401 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    29461 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    29521 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacac aaaaacgcaa
    29581 tatcagctat caactgttca tcaatgcacc ttatgaccga ctggtgacca acaatgttcg
    29641 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcagggatgc gtgtggagat
    29701 gggctcattg acaacgctgc tgagtggcgg tgtcagcttt gatgtgccgg aaggtctgga
    29761 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    29821 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    29881 cggtctgcaa ccgggagctc cggtagagtt ccggggtatt cgcctgggta ccgtaagcaa
    29941 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    30001 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    30061 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    30121 ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg caataaccgg
    30181 tattcgtgaa tttaatggtt atcagattat cccgaccgtt agcggcggcc tggcgcaaat
    30241 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    30301 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    30361 gctggatagc atgaacaaga tcctcgctag ccagtcgatg cagcagttgc cgacggatat
    30421 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    30481 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    30541 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    30601 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    30661 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    30721 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcagatc
    30781 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    30841 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg caacaccctg
    30901 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    30961 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    31021 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    31081 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct ggccggtgtc
    31141 tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta attataaaga
    31201 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtc tctaacgtgc
    31261 ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt agctcacaaa
    31321 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    31381 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    31441 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    31501 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    31561 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    31621 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    31681 taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg cggtatagat
    31741 cagacgacca tcaaccagca cttcgccatc cgccaggccc ataatcagac gacggttaac
    31801 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    31861 gaatttcact tcgccaacgc ccaacgcgcg gcctttacct tcgccgccca gccagccgag
    31921 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    31981 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    32041 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    32101 catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    32161 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    32221 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    32281 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    32341 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    32401 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    32461 ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg aacgttagct
    32521 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    32581 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    32641 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    32701 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    32761 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    32821 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    32881 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    32941 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    33001 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    33061 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    33121 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    33181 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    33241 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    33301 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    33361 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    33421 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    33481 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    33541 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    33601 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    33661 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    33721 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    33781 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    33841 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagtgcg
    33901 aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    33961 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    34021 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    34081 gcactacagc acacgtagaa agcaccgaca atactcctgg catgggcgtt aaagctcaca
    34141 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    34201 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    34261 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    34321 tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg tgctggtaaa
    34381 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    34441 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    34501 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    34561 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    34621 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    34681 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    34741 gaacttaagc ttgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    34801 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    34861 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    34921 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    34981 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    35041 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    35101 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    35161 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    35221 aggaaacacc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    35281 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    35341 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    35401 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    35461 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    35521 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    35581 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    35641 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    35701 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    35761 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    35821 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caatattaac
    35881 tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct acaacgtagt
    35941 tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg tataaggtat
    36001 gtttaatctt ttttgtcagc gacaatttac agaagaaaat cgcggaaacc gcttcagaca
    36061 agcctccgca aggaaaatta gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc
    36121 caggattaat cctaaattta cttaatgata caaattagag tgaattttta gcccggaaag
    36181 ttgtctcgtg gcgtgagagg atgcgcttac cggacgcata ataaacccca tagcgttacc
    36241 ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca gtcaaatcat ctgccaacca
    36301 accgatcacc acactgtaat tgcccgtgcg taaagcgcga accattgact ccacagtgtg
    36361 gcaaggggag agctggctaa tctgcattac tttcgttaag ggtagcccag atgcctgaac
    36421 ccattcccga ctcagttttt gttgcggtgt taaccagagt tgccagcgcg attgctgacc
    36481 gagttgctgt aacaatggca acagtagaag ttgcgtcatc atgggctgat cttcgcgata
    36541 gacaacttca ctgataagcc cggctgtagt gttttccgta gagacacgcg caattttact
    36601 tgctgcggat gagaacgacg aagaacgatg tgcatagcct gaagtgtaca taatcaatcc
    36661 agcccctgtg agttactgta tggatgtaca gtacatccag tgacaacaaa gatcaaccct
    36721 attttcggaa agagcctcgc aaattttgtc gttggtgacg ggaaaacata aattaatctt
    36781 gccccttaag aataagttgc ctattttcgt agttaacgga tccgttaatg tgaatcattc
    36841 ttttatgtta tgattttaaa aggaatttta tgaaaagcct ctcctataag cggatctata
    36901 aatcacaaga atacctggca acgttgggca caattgaata ccgatcattg tttggcagtt
    36961 acagcctgac cgttgacgac acggtgtttg cgatggtttc tgatggtgag ttgtatcttc
    37021 gggcttgtga gcaaagtgca cagtactgtg taaaacatcc gcctgtctgg ctgacatata
    37081 aaaagtgtgg ccgatccgtt accctcaatt actatcgggt tgatgaaagt ctatggcgaa
    37141 atcaactgaa gctggtgcgt ctgtcgaaat attctctcga tgcagcgctg aaagagaaaa
    37201 gcacgcgcaa tacccgggaa agactgaaag atttgcccaa tatgtctttt catctggaag
    37261 cgattctcgg ggaggtgggg attaaggatg tacgggcgtt acgtatactt ggggcaaaaa
    37321 tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac agaaaagatt ctgtttatgc
    37381 ttgaaggtgc cattatcggc attcatgaag ctgcgctccc ggtggcacgc cgccaggagc
    37441 ttgcagaatg ggctgactct cttacgccga aacaggagtt tcctgcggaa cttgagtaat
    37501 ctggcgttgc agacgaccaa tctcaggcag taatgcaatc aataatccaa cttgttgtac
    37561 gaccagaggt tctttgctgt ctgcccgtgg ttcaagttgc tgcatccgct gtttcaggct
    37621 agctaatgcc tcattgacgc gttcttcatc agcaggttga tgatgtaacg cgtcatcaac
    37681 atagcaaact gcgtcatcaa gaaacgccag aatttcagga ttagttaact gctcccggtg
    37741 agcaccgagg gctgagatat agctggtaaa cgtatggtta aggcacagca accgaaacgc
    37801 ggcttcgcga atttgcgggg taacgttcgg ttcgctggac atatttgata ccaccgacgc
    37861 cagttcagca tcacggttgt gtgcatcgcg gcgggcaata cgatacgcca gacggttatc
    37921 acgcccctga tggtattgct ccagtatggc atcgagatac cggcagttgg cctctgtggc
    37981 gcgttcgagc attcgcggca gattgcgaaa ctgccagtca ggccagatgt agctcactgc
    38041 cgcccacgca atggcacaac caatcagcgt atcgattacg cgaggtaacg ctacttcaaa
    38101 accttcaccc agcaagttaa aacacagtag caccaaaagt gtgatgaaca tcgttgcatg
    38161 ggcatattgc acgttacgga aggcaaaaaa gagcacgccg gtaataacca gtagcaccag
    38221 ctgcccttcc agtgacggta caaaccacag cacaggaatg ccaatggcga taccgaccag
    38281 cgtaccaata atccttaact tcaggcggtg gcgcgtggcg ttatagtttg gctggcagac
    38341 aaacaaactt gtcagcaaga tccaataccc gtgatgcatt ccggttatct gaatgatggc
    38401 gtagccgaag cacaacacca gcgacattct taccgcatga cggaagaggg cggattccgg
    38461 cgtgaagtga cggctaagac gcagccagat atcactcaat ccgtgcgggc tatcatcagc
    38521 gagctcattt tcgtcattat tatggggcag tgcctgggcc tgttctgatt caattgttgc
    38581 cagttgggca tcaatggcgc gtaaattgtt cagcaaaaat cccagtgttt taagtaaatc
    38641 ggcaggcgcg ccgttatcgc gcatccgctc cagcgcagca tcaatatgcg taaaagcgcg
    38701 ctcaaaatgc ggatcatgtt gataaggctg acgcagcaaa atacagcgtg acagttgctg
    38761 gcacgcctgg ccctgcatcg acatcagccg ctgaaaacgg aacagcacgt cgctgtggcg
    38821 aaaatgttca cgcaatgttt gatactgaat atgagaagag ctggcacgct cgtgaatatc
    38881 ctgtgcgaca aagtaataat gcagcgtgcg acgcgttccc cgttgaccac gatcgccacg
    38941 taagcgggtc agcagcgaga gtttcgtctg attcaatgtc gccatcagct gaccgttggc
    39001 gagagccaaa tcgtacagcg gtgcctggct ttcatcttca atatcaggat caaacatgcg
    39061 cgacttgagc tcaagataac gcgccagttg ttcatagcaa cgcgccaggt tgtcctgcag
    39121 cgggcggacc gggaacagca gatgaccaat aagtgtcagg acgttgtacc agacggcacc
    39181 ggccagcaga tacatcggct gctgatacca gtgctcatac agtgatgttc ccaacatagt
    39241 gtaaatggcg atcagcaatg caccgaaggc aattgttgca tagcgttgac ccagaccgcc
    39301 gagcaaaatg aagccgctgg tagagagcgt taagccaatc gcaaatagcc agggccaggg
    39361 aaacagcaat tctactgagg ccgaggcgat aaaaaagcag aacagcgtaa tgatgaggtt
    39421 acgcaaacgt cccgccagtc ggtcatcgag atcggtcagc gccgctgcca ccatccccag
    39481 cgttagcgga atcgtcagtt ttacatcacc cagccaccac ggaaacgctg tggttccaca
    39541 aagcgcaata aaaatacgcg cgtaatacag ccaggcgctg ttccaggtat agcgtttgag
    39601 caaaggactt agcataaagg ccatattagt ggattattca aaacgacgac gtgcattggc
    39661 ttcacgcgca gcttgcgccg tttctaccga taccacgcga cgaccaaccg gccatagtgc
    39721 aatagcggca attttaaagt tcgcaatgcc gacaggaatg ccaatgattg aaatacattg
    39781 tgcgatgccc gttgcaatgt gcatcaggca taaccaccag ccaaagaaaa tcagccagaa
    39841 aatattcaat accgtaccgc cagtattcag cagcacattt ttgccagccg ggttcagttc
    39901 atcgacatga atagcttcat tgccataagg caccagagac agtttagtga tctcccagca
    39961 ggatcgtgtc agcggtaagg taaaaatcag cataatactg accagagtcg ccaacagcca
    40021 gcccagagtg gtggcaaatc cgccaagcac aaagttcaga atgttcaaaa cggtacgcat
    40081 aaaacctcgc tttactgtgg ttttcagtaa tgggcggcaa ttgtaacgtt tttttgggct
    40141 ggagcacgtt ttctctgacg gttacactga taagaaataa tttcgtgtgg atctacagag
    40201 tcatggaact gaaagcgaca acgcttggaa aacgtctggc acagcaccct tacgatcggg
    40261 cggtgatcct caatgccggg attaaagtct ccggcgatcg ccacgaatac cttattcctt
    40321 tcaatcaatt actggcgatt cactgtaagc gcggtctggt atggggcgag ctggaatttg
    40381 tactgccgga cgaaaaagtg gtgcgtctgc acggcaccga atggggcgag acgcagcgtt
    40441 tttaccatca tcttgatgct cactggcagc ggtggagtgg cgagatgagc gaaattgcgt
    40501 ctggtgtttt acgccagcaa ctggatttga ttgccacgcg cactggagaa aataaatggc
    40561 tgacgcgtga gcaaacctct ggcgttcagc aacaaatccg ccaggctatg tcggcgttgc
    40621 cgttgccggt taaccgactg gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc
    40681 aggcctggtt gaaagatatt gaaagcgctc ggttgcagca taaccaggcg tataccgaag
    40741 ccatgcttac cgagtatgcg gattttttcc gccaggtcga gtcttcaccg ctgaatccgg
    40801 cgcaggcccg ggcagtcgtt aatggcgagc attctctgtt agtgctggca ggtgcaggaa
    40861 gcggaaaaac gtcggtgctg gtggcccgtg caggctggtt gctggcgcgt ggtgaagcgt
    40921 cccctgagca aattttattg ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc
    40981 gtattcgcga acggctacat accgaagaca ttaccgcacg cacgtttcat gcgctggcgc
    41041 tgcatattat tcagcagggc agcaaaaaag ttccgatagt cagcaaactg gaaaatgata
    41101 ccgctgcccg tcatgaactc tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag
    41161 cgcaggcgaa gggctggcgg caatggctga cggaagaaat gcagtggtca gtgccagaag
    41221 gtaacttctg ggatgatgaa aaattacagc gtcgtcttgc atcacgcctc gatcgttggg
    41281 taagtctgat gcggatgcac ggtggtgcac aggcagaaat gattgccagt gcacccgaag
    41341 agattcgcga tctgttcagt aaacgtatca agttgatggc cccgttatta aaagcctgga
    41401 aaggtgcgct gaaggcagaa aacgctgtcg atttttcggg ccttattcat caggcgattg
    41461 tgattctgga gaaaggtcgc tttatcagcc cgtggaagca tattctggtt gatgaatttc
    41521 aggatatctc gccgcagcgg gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga
    41581 cgacgttgtt cgccgttggt gatgactggc aggcgattta ccgattcagc ggtgcgcaaa
    41641 tgtcgctcac caccgctttc catgaaaact ttggtgaagg cgatcgctgt gatttagaca
    41701 cgacttaccg ttttaacagt cgtatcggtg aggtggcaaa ccggtttatt cagcagaacc
    41761 caggccagtt gaaaaagccg ctaaacagct taaccaatgg agacgaaaaa gccgtcacgt
    41821 tattggatga gagtcaactt gacgctttgc tggataagct ctctggttat gccaaaccgg
    41881 aagagcgcat tcttatcctg gcgcgttacc atcacatgag gcctgccagc ctggaaaaag
    41941 cggcaacacg ctggccgaag ttgcaaatcg actttatgac cattcatgcc agcaaagggc
    42001 aacaggcgga ttacgtcatc atcgttggct tgcaggaggg gagtgatggt tttccggctg
    42061 cggcgcggga gtcgattatg gaagaggcgc tactgccacc ggttgaggat ttcccggacg
    42121 ctgaagaacg gcggttaatg tacgtggcgc tgacccgggc acgccatcgg gtatgggcac
    42181 tgtttaacaa agagaatccc tctccctttg tggaaatact gaaaaatctg gatgtgccgg
    42241 tagcgagaaa accgtaagaa acaggtggcg tttgccacct gtgcaatatt acttcagacg
    42301 gtccgcgaga taacgctgat aatcggggat cagaatatcg accgcgtcgt tgaaatgcgg
    42361 cgactggatt atgaagtctg ccgttgccac gttggtggcg accggaatgt tccataccgt
    42421 cgccagacgc aacaaggctt tcacgtcagg atcgtgcggc acggcattta gtgggtccca
    42481 gaagaaaatc aatacatcaa ttttcccttc tgagatcaat gcgccaacct gctggtcacc
    42541 ccccattggg ccactcaaca tcgcgttgac gttcatgccg gtcgcgcggg aaattaagtt
    42601 accggtagtg cctgttgcat acagtacgtg ttgttccagt aacggttgat gccgttccac
    42661 ccagctcatc agcatttgtt tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc
    42721 aggtaaagtg cgagtcgtca gttccataat gtacatccgt agttaacttt cctacagatt
    42781 actgtaagca cttatcgctg caagataaag accgaaaaag cctgcgcaca ggcacaaaaa
    42841 tctcaggaag atggttgttt ttccgcccat tgtaggaaag tatttcgcgt ttgtgggtca
    42901 gccagtttaa accaatactt cagccgttgt tctgtgagca cctgagactg cggtggaata
    42961 gcatccagct cggaaacacc cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat
    43021 tgcggtagtg aagcgcgttt cgcggatttg ttatagcgtt caacctcgac ctcataatca
    43081 atcgtttttg ccgttgaggt aatggcgaga atatccagct ttaccggaat cggcgtcgca
    43141 tcgccatcca acaattcaag gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc
    43201 tccaggcgag gcaggcgaaa attgacctgg ttgatgagct gggtattaaa actgaccacc
    43261 agcggtgggg agatatacag ccgttcttca ctattggaaa gatgaatcgt cttctcaacg
    43321 cgaaacacta actgatgcgg tccattatcc agttcaatgc tgtccgcacc gcgcagcaga
    43381 gagctggaaa ccttcttgcc gtcgagtacc agaagatcga catcggttga aagccgcagc
    43441 gtggtggcaa aaacggatac cggcaaacat aatgcaatca aggtggtcac gatgccggtt
    43501 ttcataggta gctcctgtca aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta
    43561 atcatactta ctaacatata gacatatttc ccgcttttgc tctcaatcat tcgtattagc
    43621 tgcatggttg gcatgtcggc tttgtcgtac actttgttaa acagtctgga ggaaataatg
    43681 aaagaaaccg atattgcagg tattttaacg tccactcaca ccattgcgct ggtgggggca
    43741 agcgacaaac ccgatcgtcc cagctatcgc gtgatgaaat atctactcga ccaggggtat
    43801 cacgtcattc cggtttcgcc aaaagttgcc ggcaaaacgc tgctgggcca gcaggggtat
    43861 ggcacgctgg cagacgttcc ggaaaaagtc gatatggtcg atgttttccg taattcagaa
    43921 gcggcgtggg gcgtggcgca ggaagctatc gccattggcg cgaaaaccct ctggatgcaa
    43981 ctgggggtga ttaatgagca agcggcagta ctggcacggg atgccgggtt aaatgtggtg
    44041 atggatcgct gcccggctat tgagatccct cgcctgggcc tggccaaata aaaaatcccc
    44101 ggaaggcaaa aaccttccgg ggatttgttc agggattagt tacgcagacg cggggcctgg
    44161 agttgtttgc ggatggtctg cgccagctca tccatggatg gctgttcagg atgttcatcc
    44221 tgcaattcgc tacttagctg ggcttcagcc aggtaggtat gaaccggtag gccgttatcg
    44281 tcctccatca ccacgtgata ccacggagcg gcgcgaagct cgtcattcac cgccagttca
    44341 tcaggcgatg gttcagagag cgaataaacc gggtcgatat ccacgaccac tccgagataa
    44401 cctaacaggg aatggcggac ctgctggccg ataccgaatt tgctggcaat catagtcacc
    44461 tcccgggaaa tctttacctt tgatataagg gtaaaattcc acatttcaag ttacatgacg
    44521 cgacaggcaa accctttcag atatagccct tccgggtagg tagcgatcac cggatgatcg
    44581 gctgcctgac ggaactgctc tataaattgt acatcacggc cggcatcaat tgcggcatcc
    44641 gcgatgattt tctgaaataa atcgctggtc atcagaccgg aacaggagaa agtcaggaga
    44701 ataccgtctt cattcagcaa ctgaatcgcc agcatgttga tatctttata gccacgacac
    44761 gcgcccatca actggctttt attctcaaca aacttcggcg ggtccatcac gataacgtca
    44821 aatttttcac cgcgatcgcg ataagtacgc agcaatttaa agacatcatc acggacaaac
    44881 tcagccttgc tcagatccag tttgttcagc tcaacgttct gccgtgcaat atccagcgct
    44941 tcctgggagg tatcaacgct gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg
    45001 aaaccaccgg tataggagaa acagttcagc acacgtttat tttcaacgta gcggcgggta
    45061 gccaggcggc tgtctcgctg gtccaggtag tagcccgttt tgtgtccgtg ctgaatatcc
    45121 accagcagct tcatgccgtg ttcttcaatc ggcagcaggg caggcggcaa ctcgccggtg
    45181 acggggcctt gggtcagctc catcccttct tttttacgta ccgcaacatc gctgcgatcg
    45241 taaatcgcac attccgggta cagcgtttgc agggcactaa ttaatgccgc gcgctgatac
    45301 tctgccccag cactcagcag ttgcagcacc agaaaattac cgaaacgatc gatagtaata
    45361 cccggcaggc catcagattc tccggcgatt aaacgatagc tgtcgaggcc atctttttgc
    45421 gccagccagt cacgccattt ttgtgcttgt tgcaaacggc gggaaaaaaa agcaatgtcg
    45481 atagactcag acgggtcaaa cgtccagacg cgcgcccgga tttgcgaagc tggcgaataa
    45541 gcgccgcgtg ctaaccattt tccctgatga tcaacaatat cgatggtttc accgaggctg
    45601 gctttacctt ccatgcgggc aacggccccg gaaaagaccc acggatggcg acgaagtaat
    45661 gatttttcgc gccctttggc taacactaaa cgtacactca taatttactt ttctgtcgat
    45721 gcaaagaaat gggcgtcatt gtccggagtt ttccatggat ttgcaacgcg tccattaagg
    45781 ataaggagag cagcaatgtc gaaagtctgc ataattgcct gggtttacgg gcgagttcag
    45841 ggcgtaggat ttcgctacac cacacagtac gaagcgaaaa gactgggttt aaccgggtac
    45901 gccaaaaatc ttgacgatgg cagcgtggaa gtggttgctt gcggtgaaga agggcaggta
    45961 gaaaaattag tgcagtggtt aaagagtggc ggcccgcgtt ctgcgcgtgt agaacgggtg
    46021 ctcagcgagc cgcatcatcc ctcgggggaa ttaaccgatt ttcgtatccg ttaaatacat
    46081 tttaccggtt ttggcaggcc agcaattttg gtggcttgct tagccggacc tttcggaaac
    46141 agtcggtata aatagcggct gttacctttt tcttcgccaa atttattcgc catcgctttt
    46201 accagcatac gaatcgccgg agaagtattg aattccagat agaaatcacg cacaaaacgc
    46261 accacttccc agtgttctgg cgacagcgaa atcccttcgt tctttgcaat caccactgcc
    46321 agcggttcac tccactggct gctttctttg agatagcctt cggtatccgt ttctatctct
    46381 ttaccttcga agatcagcat aattattact accttaatca gactggcggc agtgtaacaa
    46441 aaaacaaagc cccgcataaa gcgaggctat gaaagtgtta gcgggtgaga ttaatcgcgg
    46501 ctggcgaagc ccagaatgct cagcaggctg acgaagatgt tgtacagcga aacatacagg
    46561 ctaaccgtgg cacgaatata gttcgtttca ccgccatgaa tgatgttgct ggtttcaaac
    46621 aaaatagcgc cagaggagat cagaatgaag accgcgctga tcgccagatg cagagcaggc
    46681 agttgcagga agatattagc aaccatacca atcagtacca ccacaatacc cgccatcagc
    46741 ataccgccga ggaacgacat atctttgcgg gtggtcagca catatgcaga gcagcagaag
    46801 aacactaacg ccgttccgcc cagtgccata gcgattacgt cacccattcc ggcagacaga
    46861 taggtgttca gaattggtcc gaggatataa cccagaaaac cggtaaaggc gaatgcggag
    46921 ataatcccgg tcggcttatt cgccgtttta taggtcagga acatcaaacc atacataccc
    46981 accagcgtca gaatcagacc cggagatggc agcatcagca ccgtactggc agttgcggta
    47041 atcgccgaaa aggccagcgt caggctcagc agaaaatagg tattacgcag caccttatgg
    47101 gtgctaagca gtgatgtacg gtcatgtgaa gaactaacaa tacgatccat tgagtcactc
    47161 tctatgacag atgtaattaa ttaagcagca taatgataat gcgtaagggc acccagaagt
    47221 tttacccatc tttacgcatt tgatctggaa caggtttaac agcggattat caggtcatta
    47281 agcaaatata acgccctgag aatttcgaca ggcaaaagaa aaaggggtta gcatttagct
    47341 aaccccttat cttatctggc ggaagcgcag agattcgaac tctggaaccc tttcgggtcg
    47401 ccggttttca agaccgaaga aaaacaaata aaaatcagtg taatacgtga aattgctgaa
    47461 aagttaatca aatttaacac caagaaaaat caaggtatta ggaagttgct catagctagt
    47521 ataccaagtc tttttgccag aaatttttct ggattgtcta cttgctaacc acgagcggca
    47581 aatccagtgt tggtgtgatt tttgttttac gatcgtaaat caacacctga ttttctgttt
    47641 tatgcccgct gaaaatttgt ttgtcgcgac tgctgccttc gtaatctgag atccccttgg
    47701 cttttatgtc gtgaaaattg cacccgaacg gaacgccaac tttttgctcg gctgcgcgtt
    47761 tagcctgatt ccaccagtta ttcagcgttt tagctatgac tttcccgcct ttggttgtat
    47821 tgatcacata ttcgcatgta caggaagata catttcgggc taactggatc gctgtacgta
    47881 atcgcggtga ccattccttg atttgtttgg tgccggtttt gttttgctca atgtagatcc
    47941 ctttatccat aatatcctgc catttcaact caagcacatc accgagtctt gccgcacaga
    48001 gataggaaat ctccattgca atgcgtaact gtggaattgc ttccgcatat atcgccgcat
    48061 actgttcatc ggtgatgtaa acagtgcggg ctttaagaga gaattttctg actcctttgc
    48121 atgggttatt cttcacatac ccacgctcat atccccaccc gtatactcga ctcaggcttg
    48181 ccagttcatg atttgcctgg gtcttgctct caagccctcg tttatccatg aaaattctta
    48241 cctgctcagt tttgacatta tcagcaagca cttttccgaa taccatcagc aacgccttct
    48301 gatgttgtcg ataatctttt tgggttcggg gggccagttc tgtaaatgca ggggagtcca
    48361 taaacatgtg ccacaattta gctacggtca ttatgttgtg gagttttgct ttttccagtt
    48421 cataattttg ccagacttta gctacgctgg ttttccgcac tcttcctagc cctatacttc
    48481 ttgtacttcc ttcgggtttc cacacgtaac tgtaaccatt cgatctaacc cgcggtggca
    48541 gtacattatc ttttttattt tttcttggtc ttcccattgt tcagcgcctc aaaatcgggt
    48601 tcagcagaaa ccagttcaga tgtttttggc atcgttgtca gaccgtgtgg aatatccctg
    48661 cggagaacta tgggttcgtt tttagggccg attacaaatg ggatgccgtg cagtcttaac
    48721 tggtgttgct gttttgtgta tcgctcgtat ttcgtgatct ctttaatctc tgctggagat
    48781 agagttaatt cgtacatgtg gtcacgtgcc tttacagcat gaccgccgcc actatagctg
    48841 gtgggcggcg atatgggttg aacatcaaaa atcagcctga ctcgggagca gtttttgcca
    48901 gatggctgaa acgtatttcg cctggtaacg tgcatcgttc agagcgttat ggcgttcacc
    48961 ttcgaatgga atagtcgttc ttgcgtcgaa atccatcacc agtcccagag caaccatcgt
    49021 tcttacatcg cgatcattgg tgtaacgcca tgggcagggg atcccctgcc gttcatatga
    49081 acggcgtaaa atcacgttgt cgaaagttgc accgttaccc cagacctgaa caaaaaattc
    49141 accggagttt tcgtcgataa attcccgtaa ttgcagcagt gcatcatcta acgggatttc
    49201 atcggtaaga atggcggatt gtgcttcgcg tgactgtttc agccaccact taatggtgtc
    49261 ccgatcaatg actccgccag cagtttccag atcgatagtt ttgctgaatt ctggccccat
    49321 ctctccggtt tgcggatcga aaaatattgc acctattgag ataatcgggg catcaggatt
    49381 ttttcccatt gtttcaaggt caatcatcag atggtgccac aacctgctgg tggatgagat
    49441 ttcatgatga ccgttcactt taattaaggg atttgctgtt tcgccagttt tattatcgct
    49501 ggcgtgatac tgatcgctgc cagtgttctc cttgtgcaga tgttcagtgc cttccatttc
    49561 ctccggatca tttttctgag cttcagcctg attctcttca tcgaatgttt ccaggtaggt
    49621 tgcgttcccc atcaccgcac cacagtcagg acagttgccg ccaccggtct gaccgcatgc
    49681 ggtgcaaact ttttccagtt cctgttgcac tgctggttct ggttgttgtt cttctggccc
    49741 gttttgttgc gtatccgggc tgttttgttc cgcttctggc tcattttgtt tcacgttggg
    49801 ctgattctgg tcctcagcgt cgcgactctg gatccctttc acccatttcg gatcattcgg
    49861 gtcgctaatc ccttcaacaa attcaccacg tgatgcagca agcaatttat cggcgtcagg
    49921 ctggctgata ttggctgcct gcataatttt gtttacttcg tcagcggtaa cttttaccgg
    49981 ctctgattgt tcggaatctt cagcggtatc cacattttgc ggtaagcccg tgtatgtgcc
    50041 attttttcgg gcaaaatatt cttcttttga gatttcggta ccgccggcag ccagcgcctt
    50101 gtccagacca gaaagtttgt ttgccctgcc gtatttttcc ccgcccttat cggtaaagac
    50161 gaaataaaat ggtccttcac gctctacaga tggttcagct tccaccagga tttcattttt
    50221 ttgagtatcg gattctgccg tctccactgg agcagtttgt gctgctgacg gctggagagc
    50281 atcagtagag ctctggtctg tttcttcatg ttcaaacacg ccctttgtcg tcaggtattc
    50341 actgatatat ttgttcagtg caacgggatc tttgtgaata tcgatcggac gttcgcgaac
    50401 aaggccaaaa atagtctgac ggtcatagcc aagtgcttcg ggctgtttgc gcattgatgc
    50461 tgagatacgc ttccagtctt cgcgatcctt gtcgataacc tcatttgtcg cccagcgatg
    50521 gatggtaccg tcaatgtttc ctgcatccac atcaccgggc cagagggcgc aggccagttc
    50581 attgtcgagt gttttccatg tctgtttgta ttcgcggcga atggcaacag tgacagcccc
    50641 ggttttttca gcagcgtttt cagtgttctg ttggttgact ctggcgcggg tgagatcaac
    50701 aacagacgtg tattttccgg tttccttgcg ttcaccttcg cgacgttttt tccagatgcg
    50761 catctctgcc tgaatttcag gccatttagc gccaggctca catttatgct taacccaccc
    50821 gatggcatgc agtttaagtt caggatacat ggcgttaact tctggcattt tcatcaacgc
    50881 ttcaacgata tgtccgccga atgttgccat gtcttcctgt agtaattcct gtgcgctaat
    50941 caccatatca acggtgatgt tttcgcatgt gtcgaattta accaggactg cgttctgtac
    51001 ttcaggggac agcttgtcaa aattgacgtt catcggatcg gattctggtt cgaccggcac
    51061 aaaggaagct gactcctcat cccagcggtt ttcctgcata tattcggtat cccaggaatc
    51121 gagggcaggg cggggtatgc cgggtttatc ctcgcagaca ataaatttat aagcgcagtc
    51181 ctgagctgcc gggaattgct ccagaaattg ccagtgaaat tttgcgcgtg cgcgacgctc
    51241 atcaccggct tcaatggcag tggccaccgc aacagcgcta tcttctttta tggcctgttc
    51301 gtcaggaata gcagcgcaaa taaagatttt actcattgtg ttttaacctc attacagatt
    51361 taagggtgaa caaatccctg ccattgctgg catataaaaa tgaaaccgga tattaattac
    51421 ggtgctgttt taaagtcctg ccggtatttc gttattatta gtgtgagtag ttttatctac
    51481 cggataacag ttaccgggaa ttttttgttc tgctgctgca gccatgcatt ctttcattga
    51541 accgtataag ccagtcacca gctcaagaga ttcgccggaa acaagataaa ctgtcagaac
    51601 gagtgcaaat gttgtattca ttgtttatat cctttttgca gcaggtccag acgagccagc
    51661 attgaaggaa tgcatacttc atttaacagg tcctgctcga gttttctctg cttaatggcg
    51721 tcttcaataa atgttttgtc tccagtgata acgccaattt cgaaacgaag ttcagacgtg
    51781 ctggcattac atgataactt ttccattatc gcgtcctcaa caatgaattt tgtgatgcgg
    51841 tgcctggtgc ctccaggtga cgttaaccag ttaacaatta acgccggata cagagcattc
    51901 tcgttactca gcaaatgacc gctttaccga tttaactgtt ccgcgtgcgc tgagccgcat
    51961 tcaccgcatc acaaaattca cttttaaaaa agggcggcag agcagtcacg gagtaaaact
    52021 gataccgcca aatgtcacca gaatattgat aacagagggc gttgcagcgg ggttgtcact
    52081 taagcgtatg gtcaacctga caacccggtg tcctcaacgg ggaaagaata accccgccat
    52141 acttaccgcc gcgccatttc gcggattgcc acaaccggaa gcgcacggtc gaattaaatt
    52201 taacgacggc tctaagggag aaggacttcg ccgtgcactt tcgtgttatg ccctgacttt
    52261 tcagggatat atcctttcag taaactgtca gtgccggatt cttatctgtg tccggcgcac
    52321 gaccacacgt agcagcgtgt tggtctccat ttttaattca gcccttaatg gaggataaaa
    52381 tgccaaacaa accatgccct gcctgtaacg ctctttctgg ctttttagag aagggcggtt
    52441 attacatttt taactgtcca gaacatgttg aatttcatat atcaaaactt gacagtatca
    52501 ttacaaagcc aaatcagtat cagtctgatt tactgaataa agagttaaac gccgcccgag
    52561 attaagggcg tagtggccca ttaaaaatat tgcaaagacc aactattgtt gaactggatt
    52621 aaaccagtaa ttctctattg ctcttattgt taccatcgga tgattgccgg gggttatttt
    52681 aagttcggga ggaaaaggct ctccatcagc agccatcatt gagtatgtga aatccgccga
    52741 cgcagtccct ttcatgataa ttccgaattt ttcaagtaat gagagcagtt ccggtaatcc
    52801 tgtgtcatct gaataacttt cctcatagaa agaagcgatt gccgcaggag atgacagttc
    52861 tcgctctgca atttttttgt agttttccag aattcttttt ttgaatactt ttcctcgaca
    52921 tgaaaaccca tcaccgaggc aatccgcttt gcagtttctt cagtattctg gttgtttgtt
    52981 accgaaacag agtacaatcc ggtaaacgca tcgcgcacat tacgagccat attatcaatg
    53041 tatttcttcg ttgcagattc taattcaact tcgttcagac gttggtgaag tgcatgtgct
    53101 gcaatttcct ggatttcttt tagtaaatct ttaaattcca tctttaactc cgttagtcgt
    53161 tagattttgc cgaactggaa agcgcctgtt taaactcact gaagctgaga gcttcctcgc
    53221 cttcggcaag gccttcgaag tattcttcgt aagccttttc catgattgtg tcgaaatcca
    53281 tatcactcac ctgagtttct ttccagccag cgacgggcac cattttcggt tttaaacgtt
    53341 ttgcttttgg tatacgtcat cgcggtgaat gtaccgtcct ggttgggaaa cacgccgtac
    53401 accagagatt cgttgttgcc aagatcgata gtatccatgt tgacctcatt tccccttaac
    53461 gccgggtagc ggaacaaaaa cctgctgcat agttattaaa gttgaaccct gccgtcatgt
    53521 tcatacgcct cgggctggct acttaccccc tgaccactgc ttggtaactc gaagtattgc
    53581 ccggcgttct gtggggcggg gtgggtggtt ggtgtatgta atctacaatt aaaaactgtt
    53641 ttgtgtcaac agtttttaat tgttgttttg ggcaaaaaaa tccctcgaaa gagggagtat
    53701 gaaaattgtt caactcagat agagaaggga aattgtcgcc gagaatgtgt cacgcttaca
    53761 atctcaatgc ttgaagcagc tactctgtac aggattatgt agttagggtg ggtcacgatc
    53821 tctctcaatc cagaaacccg ttcgcttggt ggatataagt acggatgctc agatagaggt
    53881 aataccgatg tttcaatgcg tatttttagt ctacgtgctg ccggtgggtt ctccttggcg
    53941 atgtaagtta tgatctggcg caaatcatcg cgagcagacg gtagccataa aatgggtaac
    54001 attactcgct cttgttagtt acagcaattt gagcaataag attttccatt tcagccatta
    54061 cctcgtcatg tggaattgcg gggcgagtgt ctgcaaggct tgacgttact ttagtgcgca
    54121 accattcgtt gtaactgttt tcttgttcgg tagtttcgaa ttctgaaact atcggagaaa
    54181 gggctgtacc catggcataa ctcctcttct tgtactgtgg tcacgcccgg cggctttttt
    54241 gtgccgccag ccacctagca atggtttctt ccattgattt tttcttgtct ttgatttctt
    54301 gaagcatttt ttcttggtct tcctcaggaa acgcactaaa agcttggagc agttcgcgtt
    54361 ggcgaggacc aattttcatc gtgtcagggg tgaaaatttg ctcacactct tcaggaggca
    54421 ataaaaacca atgcaatgga tgccctgaaa cctcaaccag tttatccaaa cttgaggctt
    54481 taggtgtagc cttaccgctg acccattgtt gaacagtttg ttgtgtcaca ccaattctac
    54541 gggcaagctc agcctggctc catccagttt cctgaagaag cttgctgatt ctgtacatag
    54601 atacttctag ggcgctcatc attattcaat tttacaggta aatactgtta aaagcatcac
    54661 aataaaaaac tgttgattgc atacagtttt ttattgtagg ctttgcttat agtttttaga
    54721 ggagggcaaa atgctagata gcactcgcga aaaaattagg cagaaataca ctcaggctga
    54781 aataggtcgt tatatggggg tcgctcaaca gactgtttgg caatggttta gctttggcgt
    54841 tcccccaaag caggtaattc cgttatgcca actaatgaag tgggaagtta ccccgcatga
    54901 aatccgccca gatatttatc ctaacccaac cgacggttta cctgttggat gtaaggttaa
    54961 cacatcaaat gcgccggagt tgattcatga aaatcaagca tgaacacatc cgcatggcga
    55021 tgaatgcctg ggcgcatccg gacggcgaaa aagtaccggc tgcgaaaatt accaaagcgt
    55081 atttcgagct gggaatgacg ttcccggaac tgtatgacga cagccatccg gaagccatgg
    55141 ctcgcaatac tcagaaaatt ttccgctggg tggagaaaga cactcctgat gcggttaaaa
    55201 aaattcaggc gttgttacca gcgatcgaaa aagcgatgcc gcctccgctg gtggcccgaa
    55261 tgcgcagcca cagttccgct tattttcggg agttggtaga gacgaaggaa cggctggtga
    55321 aagatattga tgatttcgtt gcatcagcga tcgttctgtt cgatcagatg aatcgtggtg
    55381 gcccggcagg aaacactctg gctgtgcatt aattgggtaa taaatatgag taatgacaaa
    55441 aaattgacac tgagcgttta cgaaaacagt ccgcacatct ggcgtggcgg tttatctgat
    55501 gtggagctgg cagagtggtt gatacataaa gctaatgcgc tgctctggcg tttgtcagcc
    55561 agagaacagc gcaaagaaac cagaataaag ctggctgatg cagaagcgtg tgccgggctt
    55621 attgaggatt atacaaatct tggtatttct tcagcagaga gtgatcccat tcagcctctg
    55681 agcagggagt caatccagca cgctggttgt atggcacatc ttgtaactgc tcgtcaacat
    55741 gaggtgggta ttggatcact tccggtggga tattcgctga ttccagagct ggttgaagca
    55801 agaaaatcag ttcagaaaaa gagagatgac gcacttcaat tattgagaga gcactatggc
    55861 gcgataccag aatgcgaaca gcatcgatac cctgaaggtt atgaatggat gcagtctctt
    55921 tttgaagttc gctaatcaat atgtcgagac gaaggtatgt ttcggcgcgc agccaggctc
    55981 tgtaatccgg gagcatttcg gggctgttac accagcggtt tgttgctgca acatttaata
    56041 catgagcctg ataaaggctt ttcaaaaaat acatgtcgaa cctcctctgg ttctgtcgat
    56101 tgggaaccac agattatatc cggaggaagg ttcggcacca gatgaggtag ccatgcgtga
    56161 ttacgcaaaa gtttctccgc gattctggct gggagaaacg gggagagaac ttagaaaggc
    56221 gggtgcagaa gcgcaagttg ttgcttttta cctgatgaca tcccctcacg caaatatgct
    56281 gggtttgtat tacctgccag ttttatacct tgctcatgaa accgggcttg gtctggaagg
    56341 ggcttcaaag gggcttaaaa gggctgttga agctggtttt tgtagctatg accatgatgc
    56401 agagatggtc tgggtccatg aaatggcagc ctggcaggtt ggggaaacgt tgaagcctgg
    56461 cgataaccgt tgtacaggtg tcaggaatga gtatgcatca ttacctgaaa acgcttttct
    56521 gtcagtgttt tacgacagat ataaaacgga tttccatctg gatgtgaggc ggaataatag
    56581 ccgaaattcg gtaaggggct tcgaaggggc ttttaagggg cttcgaagcc aagaacagga
    56641 acaggagcag gagaaagaac aggaacagga caaaaacact atggttcatg gcaaaaaaaa
    56701 caccacgaac caggcagggg atgttcagac cgtcaatcct ggtcagccag caggcacgac
    56761 accggaagcc gattcagcgt atgcgctgaa agccgattcg ggcgctgtgc agcaggtgat
    56821 gaccgcaggg tcggagcaat cacaccaact gcagcagcct gaagccgatt ccgccattca
    56881 gcgggaagcc gatcgggtag tcccggaaag caccgggcag tctgtgggac gagtggatta
    56941 tccggatgtg ttcgaacagg tctggcggga atacccgttg cgtgctgggg caaacccgaa
    57001 gaaatccgct ttcagtgcct ggaaggccag attgcgcgag ggggtgccac cagagaccat
    57061 gctggatggt gtgaggcgtt acgcgagata cctggcggcg accgggaaa
//
LOCUS       DAZFYC010000016        57006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFYC010000016 DAZFYC010000000
VERSION     DAZFYC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.016"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..453)
                     /locus_tag="ACHKW5_001139"
     CDS             complement(<1..453)
                     /locus_tag="ACHKW5_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFO7510572.1"
                     /translation="MKLYIIGNGFDLHHELDTSYFSFGDYLRKKDQDIYDHLVDFMGF
                     TDLPPKLSAVDKSKHFLWSDFENSLAGLDTESVLEEFCYLLPQISSPDFRDKDWGSLS
                     IEMERILKNLTEGLLTQFKSFILQVNYPELNLNKRLRIDSDSIFISFNY"
     gene            complement(790..2151)
                     /gene="yegQ"
                     /locus_tag="ACHKW5_001140"
     CDS             complement(790..2151)
                     /gene="yegQ"
                     /locus_tag="ACHKW5_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO7510573.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2254..2550)
                     /locus_tag="ACHKW5_001141"
     CDS             complement(2254..2550)
                     /locus_tag="ACHKW5_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7510574.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            complement(2552..2848)
                     /locus_tag="ACHKW5_001142"
     CDS             complement(2552..2848)
                     /locus_tag="ACHKW5_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7510575.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            complement(3057..3389)
                     /gene="yegP"
                     /locus_tag="ACHKW5_001143"
     CDS             complement(3057..3389)
                     /gene="yegP"
                     /locus_tag="ACHKW5_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO7510576.1"
                     /translation="MAGWFELSESSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(3580..4302)
                     /gene="baeR"
                     /locus_tag="ACHKW5_001144"
     CDS             complement(3580..4302)
                     /gene="baeR"
                     /locus_tag="ACHKW5_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO7510577.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(4299..5702)
                     /gene="baeS"
                     /locus_tag="ACHKW5_001145"
     CDS             complement(4299..5702)
                     /gene="baeS"
                     /locus_tag="ACHKW5_001145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO7510578.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(5699..7114)
                     /gene="mdtD"
                     /locus_tag="ACHKW5_001146"
     CDS             complement(5699..7114)
                     /gene="mdtD"
                     /locus_tag="ACHKW5_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510579.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(7115..10192)
                     /gene="mdtC"
                     /locus_tag="ACHKW5_001147"
     CDS             complement(7115..10192)
                     /gene="mdtC"
                     /locus_tag="ACHKW5_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO7510580.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTADEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRSSLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRMRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(10193..13315)
                     /gene="mdtB"
                     /locus_tag="ACHKW5_001148"
     CDS             complement(10193..13315)
                     /gene="mdtB"
                     /locus_tag="ACHKW5_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO7510581.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(13315..14562)
                     /gene="mdtA"
                     /locus_tag="ACHKW5_001149"
     CDS             complement(13315..14562)
                     /gene="mdtA"
                     /locus_tag="ACHKW5_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO7510582.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLSSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            14841..14897
                     /locus_tag="ACHKW5_001150"
     CDS             14841..14897
                     /locus_tag="ACHKW5_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7510583.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15169..15225
                     /locus_tag="ACHKW5_001151"
     CDS             15169..15225
                     /locus_tag="ACHKW5_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7510584.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            15498..15557
                     /gene="ibsA"
                     /locus_tag="ACHKW5_001152"
     CDS             15498..15557
                     /gene="ibsA"
                     /locus_tag="ACHKW5_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO7510585.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            15778..16437
                     /gene="yegL"
                     /locus_tag="ACHKW5_001153"
     CDS             15778..16437
                     /gene="yegL"
                     /locus_tag="ACHKW5_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7510586.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            16434..17195
                     /gene="pphC"
                     /locus_tag="ACHKW5_001154"
     CDS             16434..17195
                     /gene="pphC"
                     /locus_tag="ACHKW5_001154"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO7510587.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            17192..19132
                     /gene="yegI"
                     /locus_tag="ACHKW5_001155"
     CDS             17192..19132
                     /gene="yegI"
                     /locus_tag="ACHKW5_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO7510588.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(19144..20496)
                     /gene="yegD"
                     /locus_tag="ACHKW5_001156"
     CDS             complement(19144..20496)
                     /gene="yegD"
                     /locus_tag="ACHKW5_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7510589.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            20630..21487
                     /gene="alkA"
                     /locus_tag="ACHKW5_001157"
     CDS             20630..21487
                     /gene="alkA"
                     /locus_tag="ACHKW5_001157"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO7510590.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(21526..24843)
                     /gene="dgcE"
                     /locus_tag="ACHKW5_001158"
     CDS             complement(21526..24843)
                     /gene="dgcE"
                     /locus_tag="ACHKW5_001158"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7510591.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHTGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            25161..25802
                     /gene="udk"
                     /locus_tag="ACHKW5_001159"
     CDS             25161..25802
                     /gene="udk"
                     /locus_tag="ACHKW5_001159"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO7510592.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            25894..26475
                     /gene="dcd"
                     /locus_tag="ACHKW5_001160"
     CDS             25894..26475
                     /gene="dcd"
                     /locus_tag="ACHKW5_001160"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO7510593.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            26497..28350
                     /gene="asmA"
                     /locus_tag="ACHKW5_001161"
     CDS             26497..28350
                     /gene="asmA"
                     /locus_tag="ACHKW5_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO7510594.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(28801..30384)
                     /gene="yegH"
                     /locus_tag="ACHKW5_001162"
     CDS             complement(28801..30384)
                     /gene="yegH"
                     /locus_tag="ACHKW5_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7510595.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(30585..30734)
                     /locus_tag="ACHKW5_001163"
     CDS             complement(30585..30734)
                     /locus_tag="ACHKW5_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510596.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            31043..32182
                     /gene="wza"
                     /locus_tag="ACHKW5_001164"
     CDS             31043..32182
                     /gene="wza"
                     /locus_tag="ACHKW5_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO7510597.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKIHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            32188..32631
                     /gene="wzb"
                     /locus_tag="ACHKW5_001165"
     CDS             32188..32631
                     /gene="wzb"
                     /locus_tag="ACHKW5_001165"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO7510598.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            32634..34796
                     /gene="wzc"
                     /locus_tag="ACHKW5_001166"
     CDS             32634..34796
                     /gene="wzc"
                     /locus_tag="ACHKW5_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO7510599.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVQPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            34974..35813
                     /gene="wcaA"
                     /locus_tag="ACHKW5_001167"
     CDS             34974..35813
                     /gene="wcaA"
                     /locus_tag="ACHKW5_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO7510600.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            35816..36304
                     /gene="wcaB"
                     /locus_tag="ACHKW5_001168"
     CDS             35816..36304
                     /gene="wcaB"
                     /locus_tag="ACHKW5_001168"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO7510601.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGDNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            36301..37518
                     /gene="wcaC"
                     /locus_tag="ACHKW5_001169"
     CDS             36301..37518
                     /gene="wcaC"
                     /locus_tag="ACHKW5_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO7510602.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            37493..38710
                     /gene="wcaD"
                     /locus_tag="ACHKW5_001170"
     CDS             37493..38710
                     /gene="wcaD"
                     /locus_tag="ACHKW5_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO7510603.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWSAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            38721..39467
                     /gene="wcaE"
                     /locus_tag="ACHKW5_001171"
     CDS             38721..39467
                     /gene="wcaE"
                     /locus_tag="ACHKW5_001171"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO7510604.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            39483..40031
                     /gene="wcaF"
                     /locus_tag="ACHKW5_001172"
     CDS             39483..40031
                     /gene="wcaF"
                     /locus_tag="ACHKW5_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO7510605.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            40058..41179
                     /gene="gmd"
                     /locus_tag="ACHKW5_001173"
     CDS             40058..41179
                     /gene="gmd"
                     /locus_tag="ACHKW5_001173"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO7510606.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            41182..42147
                     /gene="fcl"
                     /locus_tag="ACHKW5_001174"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             41182..42147
                     /gene="fcl"
                     /locus_tag="ACHKW5_001174"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO7510607.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            42150..42629
                     /gene="gmm"
                     /locus_tag="ACHKW5_001175"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             42150..42629
                     /gene="gmm"
                     /locus_tag="ACHKW5_001175"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO7510608.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            42626..43849
                     /gene="wcaI"
                     /locus_tag="ACHKW5_001176"
     CDS             42626..43849
                     /gene="wcaI"
                     /locus_tag="ACHKW5_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO7510609.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAVTVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVAEADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGICQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            43852..45288
                     /gene="cpsB"
                     /locus_tag="ACHKW5_001177"
     CDS             43852..45288
                     /gene="cpsB"
                     /locus_tag="ACHKW5_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO7510610.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            45481..46851
                     /gene="cpsG"
                     /locus_tag="ACHKW5_001178"
                     /gene_synonym="manB"
     CDS             45481..46851
                     /gene="cpsG"
                     /locus_tag="ACHKW5_001178"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO7510611.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPNGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPSAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKDKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LALLNE"
     gene            46906..48300
                     /gene="wcaJ"
                     /locus_tag="ACHKW5_001179"
     CDS             46906..48300
                     /gene="wcaJ"
                     /locus_tag="ACHKW5_001179"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO7510612.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGIYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            48302..49780
                     /gene="wzxC"
                     /locus_tag="ACHKW5_001180"
     CDS             48302..49780
                     /gene="wzxC"
                     /locus_tag="ACHKW5_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO7510613.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            49852..51132
                     /gene="wcaK"
                     /locus_tag="ACHKW5_001181"
     CDS             49852..51132
                     /gene="wcaK"
                     /locus_tag="ACHKW5_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO7510614.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLYLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLVVAAQQKTVAITLRELAPFDKRLGTTQHAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNAQLNEAVSRERQAGMQMVQSVLERIGEVK"
     gene            51129..52349
                     /gene="wcaL"
                     /locus_tag="ACHKW5_001182"
     CDS             51129..52349
                     /gene="wcaL"
                     /locus_tag="ACHKW5_001182"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO7510615.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            52360..53754
                     /gene="wcaM"
                     /locus_tag="ACHKW5_001183"
     CDS             52360..53754
                     /gene="wcaM"
                     /locus_tag="ACHKW5_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO7510616.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRHILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNKYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGIFMAKEETLLSFANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            53929..54822
                     /gene="galF"
                     /locus_tag="ACHKW5_001184"
     CDS             53929..54822
                     /gene="galF"
                     /locus_tag="ACHKW5_001184"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO7510617.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            55194..56267
                     /gene="rfbB"
                     /locus_tag="ACHKW5_001185"
     CDS             55194..56267
                     /gene="rfbB"
                     /locus_tag="ACHKW5_001185"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7510618.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNSNEALPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVMTQGVVGETYNIGGRNEKKNLDVVNTICDL
                     LDEIVPKQGSYRDQIIYVTDRPGHDRRYAIDASKISDELGWKPQETFESGIRKTIGWY
                     LNNLEWCRRVQDGSYQRERLGLQ"
     gene            56268..>57006
                     /gene="rfbA"
                     /locus_tag="ACHKW5_001186"
     CDS             56268..>57006
                     /gene="rfbA"
                     /locus_tag="ACHKW5_001186"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO7510619.1"
                     /translation="MKGIILAGGSGTRLYPVTMAVSKQLLPVYDKPMIYYPLSTLMLA
                     GIRNILIISTPQDIPRFMGLLGDGSQWGLQLEYKIQNSPDGLAQAFILGEDFIGNDNC
                     ALILGDNIFYGHDLQKHLEIALSKDKGATVFAYHVKDPMRYGVVEFDKQGKAISLEEK
                     PEIPKSNYAVTGLYFYDNNVVEIAKSLKPSKRGELEITDINRLYLERGELSVAMMGRG
                     YAWLDTGTHESLIEASNFIQTIEARQGL"
BASE COUNT        13913 a        14532 c        15188 g        13373 t
ORIGIN      
        1 ataattaaaa ctgataaaga tagagtcaga atcgatacgc aatcttttat ttaaattaag
       61 ttcggggtag ttaacctgca atataaatga cttgaattgt gttaaaagcc cttcggttaa
      121 attttttagt atacgttcca tttcaatgct taatgagccc cagtccttgt cacgaaaatc
      181 agggctgcta atttggggta aaagatagca aaactcttcg agcactgact ctgtatccag
      241 acccgctaag ctgttttcga aatcagacca aagaaaatgt tttgacttat caactgcgga
      301 aagtttaggg ggtaagtcag taaatcccat aaaatcaacc aagtgatcgt aaatgtcttg
      361 gtctttcttc cgaagataat ccccaaatga gaaatagctc gtgtcaagtt cgtgatgcag
      421 atcaaaccca ttcccgatga tgtatagctt catgcttcca actccataac agtattggcc
      481 tttaaggttg cgataacttt caagtattag cgtgtttgcc tttgaagaaa accccgcttt
      541 gtggacgggg tttgtttgat gtggtcaatg tgtggacatt gtaagaaata aatccattta
      601 tttcagtaga ttacacgcaa aaaataagcc cgtgtaaggg agattacaca ggctaaggag
      661 gtggttcctg gtacagctag cattttatgg gttatgtttt tcagcgaaac ggatgataac
      721 cttaataaat gcagctgtat gtgatcggtt tctaagaatt ttccatccgg gaaaaataat
      781 cgaaattaat cacttaccgt ggggattacg cgtggtttcc ccggagaaat tacgcatcag
      841 cagcgcgtaa ttgagctcaa gatcctgcgg gaccgggagc cacacagtat aaccatcgcc
      901 tggtgctatc ggcatagctt cgcctttggc gttttccatg tgctcaaggg taaaattaat
      961 gttgccttgc ggcgtcatca gctcaaggct gtcgccaacg gagaatttat ttttcaccgc
     1021 taccgccgcg aggtccccct tgcgctcacc ggtaaactca ccaacaaact gctggcggtc
     1081 agaaactgaa taaccgtatt cgtagttctg ataatcgtcg tgagtatgac gacgcaggaa
     1141 accttcggta tagccacgat gcgccagacc ttccagagtt tccagcaggc tggtatcgaa
     1201 cggttttccc gcagcggcgt catcgatagc tttgcggtaa acctgtgcgg tgcgtgcaca
     1261 atagtagaaa gatttggtac gaccttcgat tttcagcgaa tgcacgccca ttttggtcag
     1321 gcgttctaca tgggcgatgg cgcgcagatc tttcgagttc atgatgtaag tgccgtgctc
     1381 atcttcaaac gcggtcatat actcgcccgg acgctgggcc tcttcgatca taaacacttt
     1441 gtcggttggt gcgccgatac ccagcgtcgg ctcaacattt tgcaccggaa tcggctcgta
     1501 cttgtgtacg atgttgccaa catcatcttc tttcccttcc tggacgttgt actcccagcg
     1561 gcaggcgttg gtgcaggtgc cctggttcgg gtcgcgcttg ttgatatagc cagagagcag
     1621 gcagcgaccg gagtaggcca tgcacagcgc gccgtgaacg aagatctcga tctccatatc
     1681 cggcacctga ttgcggatct cttcaatctc ttccagcgac agctcgcgag agaggatcac
     1741 gcgggtcagg cccatttgct gccagaattt caccgtcgcc cagttcacgg cgttagcctg
     1801 caccgaaagg tggatcggca tttcagggaa gtgctcacgc accagcataa tcagccctgg
     1861 atcggacata atcagcgcat ccggccccat ttccaccacc ggtttcaggt cacggataaa
     1921 ggttttcagc ttggcgttgt gcggtgcaat gttgaccacg acataaaact ttttccccag
     1981 cgcgtgggct tcattgatgc cgagctgaag attttcgtgg ttgaattcgt tgttgcgcac
     2041 acgcagggaa taacgcggct ggcccgcata aacagcatct gcgccataag cgaaagcgta
     2101 acgcatattt ttcagcgttc ccgccgggga aaggagttcc ggtttaaaca taattctctc
     2161 gttctgatga caggtcagat ccgcttcacc tgatgaagcg gttaggggag tgcccccact
     2221 ttaagggcgg gcattgtagc gcaatcggcg ggtttagctc cagtgtaaat gtctggtggc
     2281 gtcctggttt tggttaagga tacgaatgac gataatttcc cctggtgatt gcaggaaata
     2341 aatgacgtgg cgttcaaaag gaagaacaaa aataccttcg cctaattcag gtctgggcgt
     2401 acctatatta ttgttactca gaatctggag aacgtctgaa agatgttcca catatctgtc
     2461 ggcttgtggt tcgccgaaat ggtgatagct gtaataccag atgccttcta aatcctcatt
     2521 ggcttttggc atgagtttaa taatacgcac attatttcct tatccctgct ttcaccttcc
     2581 gtaagaacgc gtccttttcc cacggctgag cttcaccact actcaaccct tctgcgagca
     2641 tgtcacgtaa cgcttgcagg cgagactctg cttgcttttc tcgaagtaat cgcagtgact
     2701 cacggatcac ttcgctttgg gtgcgatagt cgccggattc aattaaggac tcgataaatt
     2761 cacgcaactc atcgccgaga tcaaccgtca tcgttctggc cattttacgc ccgcctgtaa
     2821 gtaaaaatct tacatcagtg tatgtcataa accggatagc gtcacgaaag tattagaaga
     2881 tattttggca catgaacatg ttcaggagga taagtatggg atggtaaaca aagaaagcac
     2941 aacgagtgct cgtcagtgcc cactatttta atggcggcgg ttactcttaa ttattaataa
     3001 ctaagcgtta tttggatata aagccggacg ccaacaaccc acgcccggca tcgtaattac
     3061 gtattgtctt tcaccgtctg gcttgtgcca ttggctttaa cggaggcaat tccggtttca
     3121 cgagattgcg cggtggcgta catctggctg gagccgataa tttgatgatt agcggctttc
     3181 agattgaaat agaatttgcc gttacttgcc gtttttttct catagcgttc ttcttgcggg
     3241 ctgttgctac gcaccgacgc gatgcccttt tccgcagagg cttttgaggt atacagctcg
     3301 ctggtgagga tagtctcacc attgcccgct tttagcacaa accggaactg attatcactg
     3361 ctctcactga gttcaaacca accagccata atgactcctt gattgaaatt gtgtatcgaa
     3421 aacagcatct aaaggtgctg tttaccgatt aaagtatgag cgattttctg atgcctgcca
     3481 gggcggagag ataatgtcag tttgctgacc atgcgtggga taatgttgcc ggatcgggtc
     3541 tgaacgccat ctccggctaa caaaataatg tcgctaaaac taaacgatgc ggcaggcgtc
     3601 ggcttcccag cggtaaccga cgccgtaaac ggcgcggata aatgactgtt cggcgtcgag
     3661 agattccagc ttgcggcgca ggtttttaat gtggctgtca atggtgcggt cggttactac
     3721 gcggtagtcg tcataaagat gattgagcaa ttgctcgcgg gagaacactt ttcctggttc
     3781 atgagagagc gttttcaaca gacgaaattc cgcaggtgtc aggtcaagca ttttaccgcg
     3841 ccatgaagcc tgaaaacgac cttcgtcgat aatcaacggg ctttcagcat cctgttgctg
     3901 caactcgcgc tgcggtttgc aacggcgcaa aatggttttg acgcgcgcta ccacttcccg
     3961 tgggctgtac ggcttacaga tataatcatc tgcgccaatc tccagcccca gcaggcgatc
     4021 gatctcttcg atttttgccg tcaccatcac gatcggaatg tcagaaaaac gacgaatttc
     4081 ccggcacagc gtcaggccat cggtgccagg gagcatcaga tctaacagga tcagatccgg
     4141 tggtgtctgg cgcacatacg gcagtacctg atcgccgtgg ctgataagcg tcggcgcata
     4201 gctcgcagca cgcagataat caatgagcaa ctgccccagc ttcggttcat cttccacgat
     4261 caaaatacgc ggtgtgtttt cgtcgattgg taactcggtc atacttctct ctgtaaatcc
     4321 cgttccagcg gtaactctac tgtaatgctt accccgccaa aaggcgaatg ggcagcaata
     4381 atgcgaccat tatgtgcttc aacaatgttc aggcaaatcg ccagccccag cccggaaccg
     4441 ccgctggcac ggttgcggga accttcggtg cgataaaaac gttcaaacaa tttttgtagc
     4501 tgatcgtcac tgacacctgg cgcactgtcg gcaaaggtca ggcgcaccgt tttgtcgcgc
     4561 tgcccggcag agatttgcag gctgccgccg ctgtcagtgt agcgcaggct gttttccagt
     4621 aagttattga ataactgcat taaacggtcg cgatcgccaa atacggtaat actgtctggc
     4681 agggaaaatt gcagtttcag gccacgactg gcgaatcgtt cgcgaaatgc gccgcccgcc
     4741 acttccagca gtgggatcaa atctaccggt gctttttgat aggcgagagc gccttcatca
     4801 gacatcgaca actgatggag atcgtcaacc agtttggtca gtgtaccgac ctccgcctgt
     4861 aaagacgcca ccgtctccgg cgtgaattta cgcacgccat cctgaatggc ttccagttca
     4921 ccgcgcagca ccgctaatgg cgtacgcagt tcgtgagaaa tatcggccat aaaatcgcgc
     4981 cgcatttgct ggtttttctc cagtgtgctg gcaagctggt tgaagtcttg cgccagtttg
     5041 cccagttcat cttcactggt gggcgttacg cgggtagtga aatcgcccgc cgccagtttg
     5101 tgcgtgccat cgacaagtcg ttttaccggt gccagtaaac cgcgcgccag cagaaaggtg
     5161 gcgagcgccg cgagtaacgt tgccagagcg acaatcaacc agctggtttg ccgctgttgt
     5221 ttatcgaaat tgatatcagt attgcgcgtt aaccgctcaa cgggggaggc gatcaccgcg
     5281 ccaacttccg caccgttgac cagaatgggt cgccgtgtac cgtcaggtgg aatcggcgct
     5341 cgcggaccaa ccagcacttt gttgttttga tcaaccaccc agaactgggt acgccagccg
     5401 tgcggtggca tacccgggcc gggtttatct tccgaattat cgtgttcaaa tgaacgcagg
     5461 atctgaaaga caaagcgatc attgttgcgc aggaagcgcc agttgccatg ctgcgcatac
     5521 tgctcgccaa gcgcatcact taacagttgt aatcgctgtt cattaccatg cttgatgtaa
     5581 tcaataaagc cacgctcaaa actgatacgc accgcccagt gcatactgat cagcaagaca
     5641 atgcaggtgg cgaaaatcgc cagaaacagt ttgccggtaa taccgggtcg ccagaacttc
     5701 attgcgcgct cctttttcgc cgcgaaatag ctacattttg atgcgtatcg ttcggcactc
     5761 tggcaaagat gaacgccgga agggcgatga tcaacgccat gctaagccag gtgtacataa
     5821 agacggtttg tgtggtgccg ctgtcgacgc tgacatgctg tgaaccaaaa agtcccagca
     5881 acagcccggc gatagtgacg ccgatactca tcgacaattg cataatcatc gacagcaggc
     5941 tgttgccgct gctcgccaga ttgtccggga gatctttcag cgtcagggtg ttcatggagg
     6001 agaaacgcgt cgagttgacc atcccttgta aaaacaggac gaacggcaaa acgtagtacc
     6061 agcccagcag ggcggtagtc ataaacaaca gggtgaccag cgacagaccc agcgtggtcg
     6121 ctaccagtac ccgacgataa ccaaagcgat tcaccacctg taccacaatt cgcttcattc
     6181 ccatgctgcc aagcaccatc gggatcatca tcagtccggc atgaaacggt gagaaaccga
     6241 ggccaatttg caggaaaacc ggtgtcataa agggcaacat gccactgcca atacgtccgg
     6301 caaagctccc cgccaggccc agcgaaaagg tacgagtgcg gaacagtttc agactgaaaa
     6361 gagcgcggtt gttatttctg gcgtgcagca aataaagcac cagtgctacc acgccaactg
     6421 cgaccaggcc agcaatcgtc agcggcgata aacctgtacc tttactgccg tccagcgcca
     6481 gggttaatac cgccatgcca accgccagca ataaaaagcc ggagagatcg aagcgccgcg
     6541 tctgcatggt atagttcggc attaacatca atgtggcgat cgcaccgata atccccaccg
     6601 gaatgttgat caaaaagatc cagtgccacg acgcgtactc caccagcaga ccgccgagcg
     6661 ccggaccgag cagcggaccg acctgaccgg gtaacgtgac aaaggtcatc gccgccatat
     6721 attgctcgcg cggtacgatt ttcatcaccg tcaatctgcc gaccggcacc atcatcgcgc
     6781 cgccaacgcc ctgtaacgcg cgtgccagca acagttcgtt cagcgtgccg gaaagcgcgc
     6841 aaaacagtga accgagagta aacagcacga tggcggtaaa gaaaatattg cgcacgccga
     6901 ctttgtccgc cagccagccg ctggcgggca gcatcaccgc cacggtcagc acataagaga
     6961 caatgaccat gtgcatatgc aacggacttt ccccgaggct ttgcgccatt gagggaaggg
     7021 cggtgtttac gatggtggtg tccagcgact gcataaagaa gccgaaagcc acaatccaca
     7081 attgccagcg ggtgctgtcg ggaagatctg tcatttactc ggttaccgtt tgtttgggtt
     7141 tacgcgaaaa acgcagccgc agacggtcga aaaagagata caccaccggc gtggtataca
     7201 gcgtcagaag ctggctcatt accagaccgc cgacaatggt gatccccagg ggttgccgca
     7261 gctccgagcc gtcaccgccc gacaacacca gcggcagcgc gccaaacagc gccgccaggg
     7321 tagtcatcat aatcgggcga aaacgcagca gacaggcctg gaaaatagct tcctgcggcg
     7381 tcaggttacc gtgccgttgg gcttcaagcg caaaatcgac catcataatg gcgtttttct
     7441 tcacgatgcc gattaatagc atgatcccta tcagggcgat taggctgaac ggggcattga
     7501 acagctccag cgccagcagc gctccaacac ccgccgaggg cagggtggag agaatcgtca
     7561 gcgggtgcac ataactctcg taaaggatac ccagcacgat atacaccgtg gcgatggcgg
     7621 cgataatcag gatcacctgt gagttcatcg tctcctggaa cacctgcgct gttccggcaa
     7681 aactgccgcg caccgtcgaa ggcacaccaa gctgggtcat tgcgcgatcg atcgccgcac
     7741 tggcgtccga gagcgatttt ccggtcggca ggttaaacga aatggtcgag gccgccgata
     7801 atccctgatg attcaccgat agtggggcat tcgccggttg ccatttagcg aaatacgaca
     7861 gcgggatcgc tttgccttcg ttattgataa cgaacatttt ttccagcgca ctgatgtcct
     7921 gggtatagcg cggatccact tccatcacca ctttatactg gttcatcggc tggtaaatgg
     7981 tcgagatttg ccgctgaccg aaggcgttat ttaacagact gttggcggct tgtacgtcga
     8041 ttcccagccg tgccatggtg tcacggtcgt aaaccagatt catctccgcg ccgttatcct
     8101 gctgatcgga gttcacgtcc gccagttccg gcaacgtcgc cagttttttg cggattttcg
     8161 gctcccattc gcgcagtgcc gccaagtcgt cggataacaa cgtgtactgg tagctggcgt
     8221 tcgactgacg cccaccaacg cgaatatcct gtaccgccat caggaacaga ttcgcccccg
     8281 gttctttcgc cagttttacg cgcagacggt cgataatttg ctgcgccgtt tcgctgcgtt
     8341 cgtcgcgtgg cttgagggtg ataaacatca tcccgctgtt cactcgcgaa ccgcctgtaa
     8401 agccggtgac attatccact gccggatcgt cacggataat tttcatgaaa tcctgcaact
     8461 taccgcgcat cgcctgaaac gaaatactct gatccgcctg aataccgccc atcaacacgc
     8521 ctgtgtcctg ctccgggaag aaggttttcg ggatcgagat atacagccag atattcagcg
     8581 caatggtgcc aagcagcacc acgcccacca gacgggtatg attgagcacc catttcagtg
     8641 acttgccgta gccttgttgc agggctacca acatgcgacc aaaaccacgc attcgctttt
     8701 gctcgcgcgg cttgctggct ttcagcatcc agccacacat cattggcgtt aatgtcagag
     8761 aaaccagcaa cgatatacca atggcgacag aaagcgtcac ggcaaattcg cgtaacagtc
     8821 ggcccggcaa tccgcccatc aacagcagcg gcaggaacac cgccaccagt gacagactca
     8881 tcgacagcac cgtaaaaccg acttcgcgag taccttgcag tgcggcttgc aacggtttca
     8941 ttcccgcttc cagatgacgg gcaatgtttt ccagcaccac gatggcgtca tccaccacga
     9001 aaccagtagc gatggtgagc gccattaacg aaaggttatt gagactgaat ccgcacaggt
     9061 acatcgccgc aaacgtacca atcagcgaaa ccggcaccga aacggcggga ataatagtgg
     9121 cgcgacccga gcgcaggaat aaaaatacca ccagaatcac cagcgccacc gagataatca
     9181 gcgtttgctc gacttcttcc agcgaggagc gaatggtggg ggagcgatcc tgggcaattt
     9241 gcagatcaat cgccgccgga atggtttcct gcaactccgg taattttgcc cggatgctgt
     9301 caaccgtctg gataatattg gcttccggca gcttgcggat catcagcaaa atagccggtt
     9361 tggcattggt catcccggcg ttacgtacat cctgtacaga gtcggtcact gtcgccacat
     9421 cgcccagacg aaccgcgccg ccgttgttgt aatgaataat cagcggctga tattcgtcgg
     9481 cggtttttag ctcatcattg gtctggatct gccagcggtg agtgccatct tccagcgccc
     9541 cctgcggttt acgcacgttg gcattgctga tggcagtgcg tacgtcgtcc agcgagacgc
     9601 cctgattaaa cagcgcctgc ggattcagcc cgacgcgtac ggcgggcagt gagctgccgc
     9661 cgacatcgac atcaccaaca ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg
     9721 caaaatcgta cagttcaccc tgcgaataag tatcggatgt cagcgtgagg atcataattg
     9781 gcgcatccga cgggttagct ttgcgatagg tcggacggct gggcatcccg ctgggcagca
     9841 aactttgtgc agcgttgatc gccgcctgca catcgcgcgc cgcgccgttg atatcccggt
     9901 caaaatcaaa ctgcaaaata atacgcgtgc tgccgagcga actgctggag gtcatttcac
     9961 tgactccggc aatgcgccca agtgagcgct ccagcggcgt ggcaacggaa gacgccattg
    10021 tttctggtga cgcaccgggc agcgaggcgc tgaccataat caccggaaaa tcgacctgcg
    10081 gcagcggggc gaccggcagc atacggaagc ccagtatgcc gcacagggta atggcaaccg
    10141 acagtaaaat cgtcgccacc gggcggtaaa tgaagagggc aaaaaacttc acttacgcct
    10201 cctcttcatg acgggcaaag cggcttttgg tccacaatgc caggcggtcg aacagcaaat
    10261 aaatcaccgg cgtggtaaac agcgtcagca cctggctgac aatcagaccg ccgaccatgc
    10321 cgatacctaa cggacgacgc agttccgcgc cgaccccggt actcaacatc agcggcagcg
    10381 cgccaagcag agccgccaga gtggtcatca ggatcggacg aaaacgcaac agacaagcct
    10441 ggtagattgc ctcgcgcggc gacatgcctt gctcgcgctc ggcagccagc gcgaagtcga
    10501 tcatcatgat ggcgttcttc ttcacgatac cgatcagcaa aataatgccg ataatcgcaa
    10561 tcacatccag ttcgctacca gcaatcagca acgccagcag tgcgccaacc cctgcggtgg
    10621 gtagcgtcga gagaatggtg atcgggtgaa taaagctctc gtacagaatg ccgagcacga
    10681 tatacatcgc caccaccgcc gcgacaatca gccagacagt gctgcccagc gccgactgga
    10741 aggcgagggt gctgccctgg aactgcgtgg tgatatccac cggcagattc agcgtctttt
    10801 cggtgtccat aatcgcctgc accgcatcgc ccagcgaata gttatccggc acgttaaagg
    10861 agatggtcgt taccgggaac tgatccagat ggttgatgga aagcggcgca aaacgctgtt
    10921 caactttggc aattgagctt aacggcacca cgccgccgtc gctactggtc aggcgaatgg
    10981 tatccagcgc cgcgaggccc ggggtaattt cggtgttgtg ctccagcact acgcgatact
    11041 ggttagcctg agtataaata gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt
    11101 tatcgacatc cgccatgctg atccccagac ggctggcgct gtcgcgatca acattgacat
    11161 acgccaccag ccctttgtcc tgccagtcgc tggagacatc agaaagctgt ggcagttgct
    11221 ggagtttttc catcaactgt ggcacccagg tactgagcgc atccagtgag gtggcctgca
    11281 aggtaaactg gtactgggtg cggctgacct gagtatcaat agtcagatcc tgcgttggtt
    11341 gcaggaagag atcgacgccc ggcactttat ccaccgccgt ttgcagacgg gcaatgactt
    11401 tttgcactcg gtcatcacgt tcatccagcg gtttgaggtt gatttgtaaa cgtgcactat
    11461 tcagcgacgg gttagtgcca tcaacgccaa caaatgaggt caggctttgc actgccggat
    11521 cctgcaaaat cacgtccgcg acctggcgtt gtcgctgggc catattggca aaggagctgg
    11581 attgcggtgc ctgcaaagtg ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt
    11641 tcggaatgaa cacccataag agaacgctaa gcagcagcgt gctgagcgcc acgcttaagg
    11701 tcagccacgg atgattcagc accttcgcca gtccacgacc ataggcggcg attatcctgt
    11761 cgaacatttt ttccgaggca cgggagaatc ggttctgttt acgcagcgac tcctggctga
    11821 gcatccgcgc gcacatcatc ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg
    11881 ctaccgccag ggtaatagca aattcgcgga acagtcgccc gacgatatcg cccataaaca
    11941 gcagtggaat caacaccgca atcagcgaga aggtcagcga gataatggta aagccgattt
    12001 cacccgcgcc tttgagcgcc gccgccaacg gtttttcgcc tttttcgata tagcgggaaa
    12061 tgttttcgat cactacgatg gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca
    12121 tcagtgtcag gttattgatt gaaaaatcga gaaacaccat aaccgcaaaa gtgccgatta
    12181 acgacagcgg tacagcaaca ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt
    12241 agatgatcat gactaccagc gcgatagcca tcatcaattc aaactgagta tcatcgacgg
    12301 atgcgcgaat attggtggtg cgatcggaaa gcactgtcac cttcaccgat ttcggcagac
    12361 tctcggtgag ctgtggcagc atctgccgga tgctgtcggc ggtggagata atgttagcac
    12421 cgggctggcg ctgaacattc atcacaatgg cctgttcttt gttcgcccac gcgccgagcc
    12481 agctgttttc tgctccttgc tctacggttg cgacatcgcc cagacgaatc ggtgcgccgt
    12541 tctggtaggc gatgattagc tggcgatact cttcggcgga ttgcatctgg tcgttagcgg
    12601 aaagcgtgac cgcacgggaa gggccgtcga ggctaccttt tgccgagttg acgttagctc
    12661 cggtaatggc ggtgcgcacg gtttcgctgg tcaggccgag ggcggcaatc gcctgggcgt
    12721 taagtttgac gcgaacagcc ggacgctgac cgccggaaag cgtcaccagt ccgacaccgg
    12781 aaatctgcga gattttctgc gcgacgcggg tttccaccat atcttctacc tgggtcatcg
    12841 gcatggcggt tgaggtgacg gcgagcgtca tgatcggcgg atctgccggg ttcactttgc
    12901 tgtaaaccgg cgggttaggc agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg
    12961 cctgcacttc ctgctcggcg acatcgagcg gtaatgttag ctggaactgc aaagtgataa
    13021 ctgacgcacc gccggaactt tgcgacgaca tctgtttcag gccagacatc tgcccgaact
    13081 ggcgttcgag cggcgcggta acggcagagg tcatgacatc cgggctggca cctgggtaga
    13141 gcgtgaccac ctgaatggtc ggatagtcca cttccggcag cgccgaaacg ggcagggcgc
    13201 gataaccgat aatcccggcg agtaagatcg ccaccatcag cagcgtggtg gccacaggac
    13261 gcataataaa caggcgcgac gggccgcctg tgctgctcgg gggtaacacc tgcatcagga
    13321 gcgtgctcct tttttcgcgt attcgcggct ggtggctttc tcttccggag tagtggcgct
    13381 ctgggcttcc accacttcca ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac
    13441 cacgcgatcg cccgcagaaa tacctgcacg gatcaccact ttctgactgt cctgaatgcc
    13501 cggcgtcact agatgtttgc tgaccttgtt ttcgctactc agcacccaga caaaatggcc
    13561 ttcattgccc atttgcaggg cggccgttgg gatcactacg gcgttttgtt cggtgtcgac
    13621 taacatgcgc gcgttaacaa actgattggg aaacagcgca tcatcctgat tattaaagcg
    13681 tgctttcact ttaatcgtac cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt
    13741 gccttcactt aatttcttcg agttggtgcg atcccaggct tctaccacca gcggttttcc
    13801 ggctttctgc gcctgcacga cggtagcgat atcgctttcc ggcagggtaa agagcaaatc
    13861 gataggatgc gtctgggtga tcaccacgat cccggtggta tcaccactgg agatttggtt
    13921 accaacatca acctgcttga gaccaacgcg accatcgact ggtgcggtga tgcggctcca
    13981 gtcgagttgc agctgcgcgc tggcgacgct tgcttcatca gccttaatgg tgccttcggt
    14041 ttcactgacc agcgcctgtt gggcatccag ctcctgacgg gagacgagat tggtttttgc
    14101 cagttgctga taacgcgcca gatcacggcg ggcgttagca agcgtggctt tatcttttgc
    14161 cagttggccc tgggcttgtg ctaatgcaac tttgaactgg ctggggtcaa tttctgccag
    14221 taaatcgcct gctttgacct gctggccttc ctggaaatgt aacgccatca gttgaccgtc
    14281 cacgcggctg cgcaccgtaa cagtattagc ggcggtaatg gtgccaagcc cggtgaggta
    14341 acgcggaact gcctgttcta cggcggtcgc cgcctgaacc ggggctaatg ggccggaacg
    14401 cataccacgg cgaccacccg ctggcgattg ctgcgcttgt ttcgtcgccc ctggggctgc
    14461 actctgggag tcattgcggc cttgccagaa ccagaatgcg gcgatggcgg cgataaccac
    14521 cacgattacg attacccaac gggatttata actgcctttc atcgttaaga gtttctcttc
    14581 ctgaaacgtt tcgcggaatg atactagtgt agccagccaa taagggagaa aaatggagga
    14641 attatgaaac actatcggga atcgtctgaa tggcgggcac atttgcgagc acgcatccag
    14701 taataacaca ggaaactatt ttatctacgc gttagcgata gactgcatac agggcgaaag
    14761 gaggtaagcc gcagatttca gccggatgct gaaacgggaa agcccctccc gaggaagggg
    14821 ccttaaataa ggaaagggtc atgatgaagc tactcatcat cgtggtgctc ttagtgatta
    14881 gctacccagc atactaagac taccagggcg ggggaaaccc cgctctaccc tcactcctga
    14941 aagtatgcct tcacgataag attgtcaatc cgcaggcttt gccgtctgcg atcctgccag
    15001 caaaaattct ttgcgagacg ttacgcaata atcacactgg aaactatttt atctgcaagt
    15061 cagcgataga ctaagtgcat ggcgaaagga ggtaagccga cgatttcagc gggacgctga
    15121 aacgggaaag cccctcccga ggaaggggcc ttaaataagg aaagggtcat gatgaagcta
    15181 ctcatcatcg tggtgctctt agtcataagc ttccccgctt actaagacta ccagggcggg
    15241 ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat tgtcaatccg
    15301 caggctttgt agtctgcgat cctgccagca aatattcttt gcgagtcgtt acgcaataat
    15361 cacagaggaa actattttat tcacgcgtta gcgatagact gcattcaggg cgaaaggagg
    15421 taagccgatg atttcagcgg gacgctgaaa cgggaaagcc tctcccggag aagagggctt
    15481 ttaataagga aagggttatg atgaagcacg tcatcatact ggtgatactc ttagtgatta
    15541 gcttccaggc ttactaagaa caccaggggg agggggaaac ctctccctaa ccctcactcc
    15601 tgaaattatg tcactccgcc cttagtgtca atcgacggct ctccgttctt gcgaggcgaa
    15661 tagcaaaatt ttctgcgccg ccgggcctaa ttctgaaagt ttcttcgttt gctcttattt
    15721 cattattgca gtcggtatga taccggacct cgataattct ctaagaaagg atttcttatg
    15781 agcgaacaaa tcacgtttgc caccagcgat tttgccagta acccggaacc acgttgcccc
    15841 tgcattttgc tgctggatgt ttctggctcg atgagcggca gacctattaa tgaacttaac
    15901 gccggattgg tgacctttcg tgatgaactg cttgccgatc cgctggcttt aaaaagagtg
    15961 gaactcggca ttgtgacgtt cggcccggtg catgtggaac aaccctttac cagcgccgct
    16021 aatttttttc cgcccatcct gtttgcccag ggcgatacac caatgggtgc ggccattacc
    16081 aaagccctgg atatggtaga ggagcggaaa cgtgagtatc gtgcaaatgg tatttcgtat
    16141 tatcgcccgt ggattttcct gattaccgat ggtgcaccaa ccgatgagtg gcaggctgca
    16201 gccaacaaag tgtttcgggg ggaagaagat aaaaggtttg ctttcttttc catcggtgtt
    16261 cagggggcgg atatgaagac tctggcacaa atcagcgttc gtcagccttt gcctctgcaa
    16321 ggattacaat tccgtgaact gtttagctgg ttatccagtt cgctgcgttc ggtttcccgc
    16381 tccacgccgg gaacggaagt tgtgctggaa gcgccaaaag gctggacgtc agtgtgagct
    16441 ggcgtctggt ttatgcatcc gctgttggaa catcgcacat cagcgcggac ttgccttgcc
    16501 aggatgcctg ccagatgcag gttgcctggc taaatgacca gcagccatta ctggtgatgt
    16561 tccttgctga tggcgcaggt agcgtttcgc agggaggaga aggcgcgacg ctcgccgtca
    16621 atgaagcgat ggcttatatg tcgcaaaaag tgcagggtgg ggaattgggg cttaatgata
    16681 tcctcgccac ggatattgtg ctgaccgttc gtcagcgact ttttgccgag gcggaagcca
    16741 aagaattagc agttcgcgat tttgcctgca cgtttctggg attgatttca tcggctaatg
    16801 gcaccttgat tatgcagatt ggcgatggcg gcgtggtggt ggattttggt cacggtctgc
    16861 aattgccgct tacgccgatg gtgggtgaat atgccaatat gacgcacttc attaccgatg
    16921 aagatgccgt ttccagactg gaaactttca ccagcactga gcgtgtgcat aaagttgcag
    16981 catttacgga tggtatccag cgactggcgt taaatatgct ggataattct cctcatgtac
    17041 ctttctttac cccgtttttc aatggactgg cgtcagcaac gcaggaacaa ctcgatttat
    17101 tacctgaatt gttaaagcag tttttgtcca gcccggcggt aaacgagcgt acggatgatg
    17161 ataaaacgtt ggcactggcg ctgtggttac catgaaaccc actttatata ctgctactgg
    17221 tgagtgcgtt acgccaggcc gtgaactggg caaaggcggt gaaggcgcgg tttatgatat
    17281 caatgagttt gtcgatagcg tcgccaagat ttatcacacg ccgccacccg ccttaaaaca
    17341 ggacaaactt gcctttatgg ctgcgacagc tgacgcgcaa ttgttgaatt atgtcgcctg
    17401 gccgcaggca acgcttcacg gtggacgagg cggaaaagtt atcggtttta tgatgccaaa
    17461 agtttctggt aaagaaccga ttcatatgat ctatagcccg gcacatcgtc gtcaacgtta
    17521 ccctcattgt gcgtgggatt ttctactcta tgttgcgcgc aatattgctt catcttttgc
    17581 tacggttcac gagcacgggc acgtcgtggg tgacgtaaac cagaacagtt ttatggtagg
    17641 tcgcgacagc aaagtggtac tgattgatag cgactccttt cagattaacg ccaatggcac
    17701 actgcattta tgcgaagtcg gcgtgtcgca ttttacgccg ccagagctac aaaccttgtc
    17761 gtcatttgtc ggctttgaac gcacagaaaa tcacgataat tttggccttg cgttgctgat
    17821 ttttcacgtc ttgtttggtg gccggcatcc ttattctggt gtgccgctta tctctgatgc
    17881 gggtaatgcg ctggagacgg atattaccca tttccgttat gcctacgcgt cagataatca
    17941 gcgacgtggt ttaaaaccgc cgccacgatc gattccgcta tcgatgttac cgggcgatgt
    18001 tgaagccatg tttcagcagg cattcacgga aagtggcgtg gcaaccgggc gtccgacggc
    18061 taaagcgtgg gtagcggcac ttgattctct gcgccaacag ttaaagaaat gtcccgtttc
    18121 ggcaatgcat gtttatcccg ctcatttgac cgactgcccg tggtgtgcgc tggataatca
    18181 aggcgttatc tattttattg atctcggcga agaggtcatt accaccggcg gtgattttgt
    18241 gctggcgaaa gtctgggcga tggtgatggc gtcagtagca ccgccagcat tgcaattgcc
    18301 attacccgat catttccaac cgactggcag gccgcttcct ttaggcctgt tacggcgcga
    18361 atacatcatt ctgattgaga tcgcactgtc agcgttatcg ctgttgcttt gcggccttca
    18421 ggcagaaccg cgttatatta ttttggttcc tgtgctgtcg gctatctgga ttattggcag
    18481 tctgacaagc aaagcgtaca aagcagaaat ccagcaacgc cgtgaggcat ttaatcgcgc
    18541 gaaaatggac tatgagcatt tagtcagcca gatccaacag ttgggcgggc tggaaggttt
    18601 tatcgccaaa cgggcgatgc tcgaaaaaat gaaggacgaa attctcgggt taccggaaga
    18661 agagaaacgc gctctggcag cacttcatga cactgcaagg gaacggcaga agcagaagtt
    18721 tctggaggga ttttttattg atgttgcctc tattcccggc gttggccctg cgcgtaaagc
    18781 ggcgttacgg tcttttggta ttgaaacagc cgcagatgtt acccgtcgtg gcgttaagca
    18841 agtgaaaggg tttggcgacc atctgaccca ggcggttatc gactggaaag cgagctgtga
    18901 acgccgtttt gtgttcaggc cgaatgaagc gataacgcca gcagacagac aagcggtaat
    18961 ggcgaaaatg accgccaaac gacatcggct ggaatcgacg ttgactgtcg gcgcgacaga
    19021 gttgcagcga ttccgccttc atgctccagc acggaccatg ccgttgatgg aacctttacg
    19081 tcaggcggca gaaaaactgg ctcaggcgca ggcagattta agccgctgct gagccatttt
    19141 cttttaacga aacaccactt ccgcccagcg tgccagcccg gcggtgacgg agccaaagtc
    19201 atcgccgcct gcaatcggaa ttcccggcaa ctgttctgcc agcgcttttt taatcagcgg
    19261 cgaacgggcg ctaccgccgg tcaggtagat aacgtccggt ttttcctggg cgttatccag
    19321 ggccagttgc acctgttcca gaatccgcgc cagtggctgg ctgagggcgc tttccagccc
    19381 ttgctggcta atcagcgtag ccagttcatc gctgataaac ggcagtgagg cgcgggtttc
    19441 cgctacgctt gaaagagcaa ttttgcactc ttctgcgcta cgcaccaggc gatagcttaa
    19501 acgctgacgc cagactttct gtagcagggc cactttttcc ggttcgcggg catcgcgtac
    19561 cagatcgtta agcagacgac cgttggcact actgtagaaa tcactctgcg caggtacgtc
    19621 gttgatggca accgcattcc accacggcag aatcggcagg gcgatgcctt tttcggtttc
    19681 gccacccatg cccagtaacg gcatcaggtt tttaaacgcc agtgcgatgt ccagatcgtt
    19741 accgccaata cggcaaccac tgtgacccag caggctggct tcacgatcga gacgcgaacg
    19801 ccactgcggc cccatcagca gcaatgaaca gtcagtcgta ccaccgccga tatccaccac
    19861 cagcacccgt ttttcttcct gcaaggtggc ttcgtaatcc agcccagccg cgaccggctc
    19921 gtactggaat accacgtccc tgaatccggc acgcttcgcc gcgcgttcca gaatcccttg
    19981 cgcctgggcg tttgcttcat cgccgcccag cccctggaag ttgatcgggc gaccaatcac
    20041 cgcctgagta atcgcttctg gcagctgcgc ctgcgcctgc tggcgaatgt gcaacatcat
    20101 tgcgcagacc agatcctcaa acagcgctac ctgctgcggt tttaagccgc tggcaccgag
    20161 gaacgatttt ggtgatttca caaaccacac ttcttctgga tcatcaatgt actgtgccag
    20221 tgaggaaaga ccgaactgca cgcttttcgc cgtaacatcg atatcttctt cgcggttata
    20281 acgaatcgcc cgacgtaaca gcgcctgcgt ttcatcgtcg tctgccggaa catcatgatg
    20341 gcggtacagc cattcgctta ccgattcacg cgttggcgcg caaagcattg aaggcagcag
    20401 cgtgctgtcg ttttccattt tcagtaattg cggtttaccg tcacgcatga ccgccactga
    20461 acagtttgct gtaccgtaat caaaaccaat aaacacgaat taatccccat gccggtgaag
    20521 aaggggcgtg actttagcga aatgttgctg tcgcgacaac cggaatatga aagcaaagcg
    20581 tagcgtctga ataacgttta tgctgaaagc ggatgaataa ggagatgcga tgtataccct
    20641 gaactggcag ccgccgtatg actggtcgtg gatgttggga tttctcgccg cccgtgcagt
    20701 gagtggtgtg gaaacggtcg ctgaggatta ttatgcccgt agtctggcgg tgggcgaata
    20761 tcgcggcgtg gtgactgcta ttccggatat agcccgccat actctgcaca taaatttaag
    20821 tgcaggttta gaacccgttg ccgctgagtg tctggcgaaa atgagccgcc tgtttgatct
    20881 gcaatgtaac ccgcagattg ttaacggtgc gttgggcaag ttaggcgcgg cgcggcccgg
    20941 attgcgttta cccggctgtg ttgatgcttt tgagcagggt gtgcgggcga ttttaggcca
    21001 actggtgagc gtggcgatgg cggcaaaatt gactgccaaa gttgtacagc tttatggcga
    21061 acggctggat gattttccgg agtatatctg cttccccacg cctcagcggc tggcagcagc
    21121 cgacccgcag gcattaaaag cgttaggcat gccgttgaaa cgggcagagg cactgattca
    21181 tctggcaaat gcggcgctgg agggcagctt accaatgaca attccgggcg atgtggagca
    21241 ggcgatgaaa acgctgcaaa cttttccggg tatcgggcgc tggacggcga attatttcgc
    21301 tttgcgtggc tggcaggcga aagatgtttt tctgccggat gattatctga ttaaacagcg
    21361 atttccgggg atgacaccgg cacaaatccg ccgttatgcc gagcgctgga agccctggcg
    21421 ttcttatgcg ctgttgcata tctggtatac ggaaggctgg caaccagacg gaacggacga
    21481 attgtaggcc tgatgcgtcg catcaggcaa tgggcaaacg ctccctcagt taatcgcgaa
    21541 ataactgcta ttcaccagca aatccagcgg ttgggcatcg gcaatcacat caccataaat
    21601 cagatcgacg ccgatgccag aaagcgtatc catcactaag ggtaaaacga ctggcccggc
    21661 gatggttttc atcccgagtc gctgagcgtg cccctgaata atcgtaatca gcatctcatc
    21721 catcaaatta ccctgcacgt tggcgcataa ctcaccatca agtagcaggt aatctgccat
    21781 attcgctttc agcgagttga agatttgcag atcgcggccc acctggctga gcactatccg
    21841 acatcccgcc agccgcagtt tttgcacgct ttctgcgtga tctaaaatcg cgtctgccgg
    21901 aataatcaga tgtaataacc gtggtggtag agggctattt tccagctgct caagcagatc
    21961 attcaccagc gtggcgctac tcaaaccggc aacggaaagg gggagggcga tgcttatgcc
    22021 tttactggca accgcttttg cggccagctg gaaaaattcg tggaataccc gtcggtcaag
    22081 agcatggcta agtgccggat cgctgaagct acgacgaaat ttttgttcat caataatctc
    22141 gccttcgcaa ctccagagtt taagcgaaat cagccacaaa ttacgcgctt ccgggatccg
    22201 cggcgaagcg acaccgtggg ccatcatcat caactgattc tctttaatca tccgccactg
    22261 ttcatcaagc gacatcgccg cccgttcgct atgtgcggca gcttgctgtg gttcgtaaac
    22321 cgtcacccgg ccccgtccac catttttgga ggcataacag gcgatatcag cctgcgacat
    22381 cacttctgcc gcctgatgat tgttgtcatc aatcaaggta atcccggcac tggcacctac
    22441 ccgatgcaca cggccttccc atataaagtg atagtcattc acggcactga taatgcgtgt
    22501 agcgataaaa cgcgcgcttt caacattaca gtctggtagc agcagaccaa attcatcccc
    22561 accgagtcgc gccagcacgt cgctggaacg cagcatactc agcatcaacg acgccagttc
    22621 gcgcagcaaa gcgtcgcccg ccgcatgccc ggcgctgtca ttcaccgctt taaagcgatc
    22681 aagatcgata aacaccaggg catgtcgctg atgtgtactg tttaccgttt gcagcagcat
    22741 acgcagttgc ttctcaaaac tggcgcgatt ggcgagatgc gtcagtgcat catgggaggc
    22801 gctgtagctc agctggcgca gcattttgcg tgattcggta acgtcctgaa tcaccagaac
    22861 cgaaccaata ttgctgccgt ccagagtact taacggcgta atactgtaat gcacgtcgta
    22921 gctgccgccg ctccggcagt gcaacaccac atcttgttca atcgcggaac gtgaggtgtc
    22981 ggcactgtaa atgttctcca tcaggggacc attgtcgcca aaggtaatat gcaacaccgt
    23041 caggagcgga acacctaatg cttcttcctg cgtccagccg ctcatcttct ccgccactgg
    23101 attcataaag gtaattttca ttgccatatc aatacagacc acggcttcgc cgatggagtc
    23161 cagcgttatg tgcaggcgct ctttttcctg aaacaatgcc tcgttaagct gtttaacctc
    23221 agtcatatcc atattaatgc cgagcaggcg ttcgacttcg ccttctttat tcagtacccg
    23281 gttggcgagg gcgcggatat ggcgaatacc gtcttttacg gtaatgcgaa attccagttt
    23341 aaagggcgag cgtgattgca acgaatcacg aatcactttt tcggcatgct ggcgatcttc
    23401 cggcagcacg cactcgtacc acacctgcca gttcggtttg atatgtggag gaatttcata
    23461 cagctcgaac atccgcttat cccagctaaa aatattcggc ttcaactccc actcccagat
    23521 gccaatcccg cccgcttcgt tagccagcgt gatgcgctcc atcagttgct gattcacctg
    23581 ttcggtgcgt tttagctcgt taatgtcttc aatctgagcg ataaaataga gcggcgtgcc
    23641 atccgtgtgg cgcaccagtg agacggcaag caacgcccag acaacatcgc cattgcggtt
    23701 gtagtagcgt ttttccattg aataggtgtt tatttcaccg cttatcagct tttcaacctg
    23761 ttggagatct ttattgagat cctccggcca ggtcagttgc tgaaaggtga gtccgcgcag
    23821 ctcttcctga ctgtacccga gaaactggca gagcgctttg ttggtttgca gccattgtcc
    23881 ctcggtgccc actaacgcca taccgatagc ggaatattcc atggcgttcc gaaaacgggt
    23941 ttcgctttcg gaaatgtgtt tgcgttccgc acgaaaggca tacatcacca tcgtcatgat
    24001 gttggcgggc agcaggatca gcaaaaacgg tagccacggc atatggctca tcaggtatgt
    24061 acgcggcgta gcaagcaggg agggatccgc ggccatcatc agcgacacca tcatcaccgt
    24121 ggtaaggaag atcaaaaagg cttccattcg tggcaggcgc acggcgctcc acatcaacag
    24181 cacaataatg aaagtaaaag gccacggcag atacagcatc gaaagccagc ttaacgtcag
    24241 tgtgatggct aacgtgagca gcgactcaaa aagcaaccgt gggttgcgat ggcgcagcag
    24301 atagtgtggt ttaaataaca atcccagcgg caccagtgcc agagcgccga tggattctga
    24361 cagtacccat atcaaaaatg ccctgagagg atcgtctccg ggcgtcagca ggacaaccag
    24421 aacaccccct agcagaggcg gaacaatggc gctgccgaat gccagacgca gccagtcagc
    24481 cagattttgc aaggggttat accacggcag caatttacgt agcagcactg ccccgaccac
    24541 ggcttcaaca atattgatgg tcgtccaggt catgttcagc gagctggtgg aaaaaagcag
    24601 gatggatgcg gcgatatttc ccagcgagca gctcagcgca attcccggcc acatgcgccc
    24661 ggtatggcga taaaacgcca ccatcatgat ggacgtcggg aaccacagtg gggcgagttg
    24721 ggtgccaaac tgcgaaagct caagcgagaa aagggtaaag ataaacgaga ctaaacctaa
    24781 actgaccagg tgaagcagcg ggtggggcag ggcaattaat acatgctgtg attgtttgct
    24841 cattaccgct ttatccgcaa gaccaggtcg ccagtattcg tttctgtgtt tatgctagta
    24901 caaaagtttt acattttata tgcgaattgc tcataaagtg acgtaaaggc ggataatttg
    24961 cgcaactgcg tataacattt tttaccttgt ataaaactga tcaacgtaat ttgccaccgg
    25021 gaattcttcg tcaacaatta actctgagcg cgcggcagcg ctattcgact ggtatcaggc
    25081 ggatgaaatc cctataattg ccgcgtttgg cgcttcgtcg cccccttcct aacatccagg
    25141 ttaatcaggt cgctaaatat atgactgatc agtctcatca gtgcgtcatt atcggtatcg
    25201 ctggcgcatc ggcttccggc aagagtctta ttgccagtac cctttatcgt gaattacgtg
    25261 agcaagtcgg tgatgaacac atcggcgtaa ttcccgaaga ctgctattac aaagatcaaa
    25321 gccatctgtc gatggaagaa cgcgttaaga ccaactacga ccatcccagc gcgatggatc
    25381 acagcctgtt gcttgagcat ttacaagcgt tgaaacgcgg ctcggcaatt gacctgccgg
    25441 tttacagcta tgttgaacat acgcgtatga aagaaacggt gacggttgag ccgaagaagg
    25501 tcatcattct cgaaggcatt ttgttgctga cggatgcgcg tttgcgtgac gaacttaact
    25561 tctccatttt cgttgatacc ccgctggata tctgcctgat gcgccgcatc aagcgtgacg
    25621 ttaacgagcg tgggcgttca atggattccg tgatggcgca atatcaaaaa accgtgcgcc
    25681 cgatgttcct gcaattcatt gagccttcta aacaatatgc ggacattatc gtgccgcgcg
    25741 gcgggaaaaa ccgcatcgcg atcgatatat tgaaagcgaa aataagtcag ttctttgaat
    25801 aagcttgata aattgtgtac cgttcagtga taacctggta tgcccttgac gtaattggcg
    25861 ttaagggagt gatgcgcgaa aggagaaaat gccatgcgtc tgtgtgaccg agatattgaa
    25921 gcctggcttg atgaaggccg tttgtcgatc aacccacgtc cgccagtgga gcgtattaac
    25981 ggcgcgacgg tggatgtacg cctgggcaat aaatttcgta ccttccgtgg tcacacggca
    26041 gcgtttatcg atctgagcgg tcccaaagat gaagtgagcg ccgcgcttga ccgcgtgatg
    26101 agcgatgaga tcgttctcga cgagggcgag gcgttctatc ttcacccagg agagctggcg
    26161 ctggcggtga cgctggagtc ggtgacgctg ccagccgatc tggtgggctg gctggacggg
    26221 cgttcctctt tggcgcgtct ggggctgatg gtgcacgtca ccgcgcaccg tatcgatccg
    26281 ggctggtctg gttgcattgt gctggagttc tacaactccg gtaagctgcc gctggcgctg
    26341 cgtccgggca tgttaattgg tgcgctgagc tttgagccgc tttccggccc ggcggcgcga
    26401 ccttacaacc gccgtgaaga tgcgaaatat cgcaaccagc agggcgcggt agccagccga
    26461 atcgataaag actaattcag gcccattgag gataccatga gacgatttct gacgacgctg
    26521 atgatactcc tggtcgtgct ggtggccggg ttatctgcgt tagtgttgct ggtgaatccg
    26581 aatgatttcc gcgactatat ggtcaagcaa gttgctgcac gtagcggtta tcaattgcag
    26641 ctcgacgggc cactgcgttg gcacgtctgg ccgcagctta gtatcctctc cgggcgaatg
    26701 tctctcaccg cccagggcgc aagccagcca ctggttcgcg ccgacaacat gcgtctggac
    26761 gtggcgcttt taccactact gagtcatcaa ctgagcgtta agcaggtgat gctaaaaggg
    26821 gcagtgatcc aactgacgcc gcagacggaa gcggtgcgca gtgaagacgc tccggttgca
    26881 ccgcgcgaca ataccttgcc ggatctgtca gacgatcgcg gatggtcgtt tgatatatcc
    26941 agtcttaagg tggcggacag cgtgctggtg ttccagcatg aagatgacga gcaggtgaca
    27001 atccgcaata tccgcctgca aatggaacaa gatccccaac atcgtggctc atttgagttc
    27061 tccgggcggg ttaatcgcga tcagcgcgat ctcacgatat cccttaacgg tacggtagat
    27121 gcttctgatt atccgcatga tttaacggcg gctattgaac aaattaactg gcagttgcag
    27181 ggtgcagatt taccaaaaca aggtattcag gggcagggga gtttccaggc ccagtggcag
    27241 gagtcacata aacgcctttc atttaaccaa atcagcttga ccgccaatga cagcacgctg
    27301 agcgggcaag cacaggtcac gctgacagag aaacctgaat ggcagctgag actgcaattc
    27361 ccgcaactga atcttgacaa cctcatcccg cttaatgaaa ccgcgaatgg tgaaaacggt
    27421 gccgcgcagc aggggcagag ccaatcaacg ttgccgcgcc cggttatttc ttcgcgtatt
    27481 gatgaaccgg cctatcaggg actgcaaggc tttacggctg atattttgtt gcaagccagt
    27541 aacgtgcgct ggcgcggaat gaattttaca gatgttgcca cgcaaatgac caacaagtcg
    27601 ggtttgctgg aaattactca actgcagggc aagcttaacg gtggaaaggt ttcactgccg
    27661 ggcacgctgg acgcgacatc aataaatccg cggataaact tccagccacg gctggaaaac
    27721 gttgagattg gtaccattct gaaggcgttt aactatccga tttcgttgac cggaaagatg
    27781 tcactggctg gtgatttctc cggtgctgac atagatgccg acgcattccg ccataactgg
    27841 caaggacagg cacatgtcga aatgaccgac acgcgcatgg aagggatgaa cttccagcag
    27901 atgattcagc aagcggtaga acgtaatggt ggtgatgtga aggccgctga aaacttcgat
    27961 aacgtgacgc ggcttgaccg ctttaccacc gatttgacat tgaaggatgg cgtcgtgacg
    28021 ttaaacgaca tgcaaggtca atcgcctgtg ctggcgctga caggggaagg tatgttgaat
    28081 ctggcagatc aaacctgcga cacccagttt gatattcggg tcgtgggtgg ctggaacggg
    28141 gaaagcaaac tgattgattt cctcaaagaa acgccagtac cgctgcgggt ttatggcaac
    28201 tggcagcaac tcaattacag cctgcaagtg gaccagctgc tgcgcaaaca tctacaggac
    28261 gaagcgaaac gtcgcctgaa tgactgggcc gagcggaata aagattcccg caatggcaaa
    28321 gatgtgaaga agttgctgga gaagatgtag attaccaggt gacctgtcct gtaggcctga
    28381 taagacgcgt aaagcgtcgc atcaggcgtt cgcggcggca ccattatatt ctgaatatgt
    28441 aagattcaag gctgttccgt tcacttgatg ttcctcgcta ctatggaacg ctcaagccgg
    28501 tgaacgtgcc agggtggctc aatcgccacc accctggcaa cccgggctcc cggcggtaaa
    28561 tcgccgctgg cgcggttccc tcatcttatt cctttcggct atcgggtacg ggcgcgaggt
    28621 aacgtcctgt aagacgcgcc ctcagcccac atccatgtgg gctgccccgg cctacaggaa
    28681 acgattcggc gatttacagc cggacccaca cccccgctgt gatgtccgtt taccacagac
    28741 aacgcttcat tgctgaagtt ataaggcgtt acgcagcatt gggtaaaatg gccgatacca
    28801 tcacacctcg tactccatct cgccatcctt acgtagcggt ataatttgca ccttctgcac
    28861 gcgatggctt tccacctgca acgttttaag caaataatcc cccacctgaa cttcttcgcc
    28921 aggttttgga atacgctgta aatattccat caacagcccg gcaatggtgt gatattcacg
    28981 tttttcatcc agcggcagcg gcacatattg caccagatct tccagcggca tatgaccatt
    29041 cgccgtccag gaaccgtccg cattcttctg aatatcatgg cgggcgtcga tctcttccac
    29101 ttcgttcggt aagttaccgg caatggtttc agtgacgtca cttaatgtca caatcccttc
    29161 caccgagcca aactcatcca ccacaaaagc aaagtgtgtg cgggcattac ggaactgctc
    29221 gagggcaggt aacaacggca aggtttccgg gaacaccagt ggctggcgaa tcaacacccg
    29281 caggttgagc ggttcgccgc gcagagactg ttgtagcagg tcgataacgt gaacaacacc
    29341 gagcaaatct tctgcgtcat caccatcagt aaccaccagc cgcgtatgct gatttcgctc
    29401 cagcaactgg cggatttctt cttccggcgc gttgagatcg atatgctcaa tatcgtggcg
    29461 cgacgtcata atgctgctga cagtacgctg gttaagatta agtacccgct caatcatccg
    29521 ccgttcctgc ggattaaaga tctgctggtt accatgatcc accagcatgg acgcggtttc
    29581 ggcgtccagt tccgcatctt ctttttgccc gctaagcagg cgcattaccg cttcggtagt
    29641 ccgctggcgt agcgtctggt tagcggaaag aaaacgtcgc cggttaaaga tagccaactg
    29701 attgagcgcc tcgatcatca ccgagaagcc aatggcggcg tacaggtagc ctttcggaat
    29761 gacgaagccg aaaccttctg ccaccaggct aaagccaatc atcaacagga agcttaagca
    29821 gagaataacg atggtcgggt gactgttaac aaattgcgtt aacggcttgc tggccatcaa
    29881 catcaggctg atagcgataa caacggcggc catcatgacc agtaaatggt cgaccatccc
    29941 gacggcggta atcaccgagt cgagtgagaa gatggcgtcc agtaccacaa tttgtgtcac
    30001 caccccccag aacttcgcac ctttgcgttg tgtggggtta ttgctgtctt ttccttccag
    30061 ccgttcgttc agctccatcg tggctttgaa caacaggaag aaaccaccaa acagcatgat
    30121 taagtcgcgg gcgctaaagg taaacgagcg gaagctgaac agcggttgcg tcagggtgac
    30181 cagccaggag attgacgcca gcagtaacag gcgcattaac atcgccagca gcagcccggt
    30241 aacccgtgcg cggtcgcgct gcttcggcgg tagtttttcg gcgaggatgg caataaagac
    30301 caggttatca atgccgagga ccagttcgat cacaatcagc gtgattaacc cggcccagat
    30361 agacggatcg gcaatccatt ccatagtaag tacaaaacct attcgttgta tgacaaatgt
    30421 cacaattcga tcatggataa atcagggcaa aaatgcaatg ttgcggcact atttatctca
    30481 cattatttga ggattataac atcgcattat ccctgaatta aaagtggtaa taataaaaca
    30541 ttatttaaga aatatcgcat gtggagacgc aatattaaat attgtcaacc taaagaaact
    30601 cctaaaaacc atattgaatg acacttaata taattcttaa aaatagccaa ttgccgaatt
    30661 gttaccttgc ctgctattcc gttagctgta acacttcctc ctgcattatt ggagagccaa
    30721 tattcaatta ccataatgtc accttatatt cggaagttaa tttccgcaat aaccctttct
    30781 catcaacgat tgtatgcaga gagggtttga aaaattaagc taaatcagtg tattggtagc
    30841 taaaaagcca ggggcggtag cgtgtctgga tgcctgaaag accagtctga attattctgt
    30901 caatagtctg cggatgaaat caattttttt aggactgatg ccagttaaat ttttattcaa
    30961 ttaactgcaa ggtaaatgtg catggcgaca ttgctattaa tagtgcacag gataattact
    31021 ctgccaaagt gataaataaa caatgatgaa atccaaaatg aaattgatgc cattattggt
    31081 gtcagtaacc ttgataagcg gttgcacagt acttccgggc agcaatatgt cgacgatggg
    31141 caaagacgtc atcaaacagc aggacgctga tttcgatctc gacaaaatgg tgaatgttta
    31201 tccgctgacc ccgcgcctga ttgaccaatt acgcccacgc ccgaatgtag cgcgccccaa
    31261 tatgacgctg gaaagtgaga tcgcgaatta ccagtatcgc gtcgggccgg gagacgttct
    31321 taatgtcacc gtctgggatc acccggaact caccacgcca gctggtcagt accgcagctc
    31381 cagcgacacc ggcaactggg tacagcctga cggcactatg ttctacccgt atatcggcaa
    31441 gattcacgtc gtggggaaaa cgctcgctga aatccgcagt gatattaccg ggcgcttagc
    31501 gacgtacatc gctgacccgc aggtggacgt taatatcgcc gccttccgct cacaaaaggc
    31561 ctatatctcc ggtcaggtga ataaatccgg tcaacaggcg atcaccaacg taccactgac
    31621 cattctcgat gccatcaacg ccgcaggtgg cctgaccgac accgctgact ggcgcaacgt
    31681 ggtgctaaca cacaatggtc gtgaagaacg catttctttg caggcgctga tgcaaaacgg
    31741 cgacctcaat cagaaccgac tgctttaccc cggcgatatt ctctacgtgc cgcgtaatga
    31801 tgatctgaaa gtatttgtga tgggtgaagt gaagaaacag agcaccctga aaatggactt
    31861 tagcggcatg accctgacgg aagccttggg taatgctgaa ggcatcgaca tgaccacctc
    31921 caacgccagc ggcatctttg tcattcgtcc gctgaaaggc gagggcgggc gtaacggcaa
    31981 gattgccaat atctaccagc tggatatgtc cgatgcgacg tcgctggtga tggcgacaga
    32041 attccgcctg caaccttatg acgtggtgta tgtcaccacc gccccggttt cccgctggaa
    32101 ccgtctgatc aatcagttgc tgccaactat tagcggtgtc cgttacatga cggatacagc
    32161 cagcgacatt cataactggt aatcgtcatg tttaacaaca ttttagttgt ctgtgtcggc
    32221 aatatttgcc gttccccgac ggcggaacgc ttactgcaac gttatcaccc ggaactgaaa
    32281 gtggagtccg ctggactcgg cgcgctggtc ggtaagggcg ctgaccctac tgctatcagc
    32341 gtcgccgcag aacatcaact gtctctggaa ggtcactgtg cccgtcaaat cagccgccgc
    32401 ctgtgtcgca actacgacct gattttgacc atggaaaagc gccatatcga acgcttatgc
    32461 gagatggcac ccgagatgcg cggcaaagtg atgctgtttg gtcactggga taacgaatgt
    32521 gaaatccccg atccgtatcg caaaagccgg gaaacgtttg cagcggtgta cacattactt
    32581 gaacggtctg cccgccagtg ggcgcaggca ttgaacgcag agcaggtata agaatgacag
    32641 aaaaagtaaa acaacatgcc gctccggtaa cgggcagtga tgaaatcgat attggtcgcc
    32701 tggtcggcac cgtcattgaa gcgcgctggt gggtgattgg catcaccgcc gtatttgccc
    32761 tctgtgccgt ggtttacact ttcttcgcca cgccgattta tagtgccgac gcactggtac
    32821 aaatcgagca aagcagcggc aattcgttag tgcaggacat tggttcggca ttagccaaca
    32881 aaccgcctgc atcggacgcc gagatccagt tgattcgttc gcgtctggtg cttggtaaaa
    32941 cggtggacga tctcgacctc gatattgcag tgagcaaaaa cacgttcccg attttcgggg
    33001 cgggctggga tcgcctgatg ggacgccaga acgagacggt gaaagtgact acctttaacc
    33061 gcccgaaaga gatggcggat caggtgttta cgcttaatgt gctggatgac aaaaactaca
    33121 ccctgagcag tgatggcggc tttagcgccc gtggacaagc gggccagatg ctgaaaaaag
    33181 aaggcgtcac gctgatggtt gaagccattc acgcccgccc gggcagtgag tttaccgtca
    33241 ccaaatactc cacgctgggg atgatcaatc aactgcaaaa cagcctgacg gtaacggaga
    33301 acggcaaaga cgcaggcgta ctgagcctga cttataccgg tgaagatcgc gaacagatcc
    33361 gcgacattct taatagcatc gcccgtaact atcaggaaca aaatattgag cgcaaatcgg
    33421 cggaagcgtc gaaaagcctc gctttcctcg cgcaacagtt accggaagta cgtagccgcc
    33481 ttgatgttgc cgaaaacaaa ctgaatgcct tccgtcagga taaagattct gttgatctgc
    33541 cgctggaagc gaaagcggtg ctcgattcga tggtgaacat cgacgcccag ttgaacgaac
    33601 tgacctttaa agaggcggaa atctccaagc tgtacaccaa agttcacccg gcgtatcgca
    33661 cgctgctgga gaaacggcag gcgctggaag acgaaaaagc caaacttaac ggtcgcgtaa
    33721 cggcgatgcc gaaaacccag caggaaattg tccgtctgac ccgcgatgtc gagtctgggc
    33781 agcaggtcta tatgcaactg ctgaataaag agcaggagct gaaaatcacc gaagccagca
    33841 ccgtcggcga tgtgcgcatt gttgacccgg caatcaccca gcctggcgtg ctgaaaccga
    33901 agaaaggact gattatcctc ggcgcaatta tcctcggcct gatgctctct atcgtgggtg
    33961 tgctgctgcg ctcgttgttt aatcgcggca tcgaaagccc gcaggtgctg gaagaacatg
    34021 gtatcagcgt ctatgccagc atcccgttgt cggaatggca gaaagcgcgc gatagcgtca
    34081 aaaccatcaa agggattaaa cgctataaac agagccagct actggcggtg gggaatccaa
    34141 ccgatctggc gattgaagcc atccgcagtc tgcgtaccag tctgcacttc gcgatgatgc
    34201 aggcgcagaa caatgtgttg atgatgacag gggtgagccc gtcaatcggt aaaacctttg
    34261 tctgcgccaa cctggcggcg gtgatcagtc agaccaataa acgggtgttg ttgatcgact
    34321 gcgatatgcg caaaggctac acccacgagc tgttgggtac caataacgtc aatggcctgt
    34381 cggaaattct gattggccag ggcgatatta ctaccgcagc taaaccgacc tctattgcca
    34441 aatttgacct gatcccgcgc ggtcaggtac agccaaatcc ttctgaactg ttgatgagcg
    34501 aacgctttgc cgaactggtg aactgggcga gtaaaaacta tgacctggtg ttgattgata
    34561 cgccgccgat tctggcagtg accgatgcgg caattgttgg tcgtcatgtt ggaaccacgt
    34621 taatggtggc gcgttatgcg gtcaacacat tgaaagaagt ggaaaccagt ctgagccgct
    34681 ttgagcaaaa tggtattccg gtgaaagggg tgattctgaa ctccatcttc cgccgcgcca
    34741 gcgcgtatca ggattatggc tattacgaat acgaatataa gtcggatgcg aaataacgac
    34801 atccgctgtc ggatgcgatg ctgacgcatc ttatccgacc taccttcacg ccgcatccgg
    34861 caagcaaacc agctccgtag gccggataag gcgctcacgc cgcatccggc aacagaaccc
    34921 gtaaacaagc ttataaaccg gcagaacatc tcgacactcc gggagaacaa cctatgaaag
    34981 acaatccgct gatttcaatc tatatgccga cctggaaccg ccaacaactg gcgattcggg
    35041 cgataaaatc ggtcctgcgc caggactaca gcaactggga gatgatcatc gtggatgatt
    35101 gctccacctc ctgggaacaa ctgcaacagt acgtcactgc cctcaacgat ccgcgtatta
    35161 cctacattca caacgacatt aacagcgggg cgtgcgcggt acgtaaccag gcgattatgc
    35221 tggcgcaagg ggaatacatt accgggatcg atgacgatga tgaatggacg cccaaccgcc
    35281 tgagcgtctt cctcgcccat aaacagcaac tggtcactca cgcctttttg tacgctaacg
    35341 actacgtttg ccagggcgaa gtctattccc aaccagcaag cttaccactg tatcccaaat
    35401 cgccgtactc acgccgcctg ttctataaac gcaatatcat tggcaatcag gtttttacct
    35461 gggcgtggcg tttcaaagag tgcttgttcg ataccgaact gaaagcagca caggattacg
    35521 acatcttcct gcggatggtg gtggagtatg gcgaaccgtg gaaagtggaa gaggcgacgc
    35581 agatcctgca tatcaatcac ggtgaaatgc agatcacctc gtcaccgaaa aaattctccg
    35641 ggtacttcca cttttaccgc aagcacaaag acaaattcga ccgtgccagt aaaaaatatc
    35701 agctgtttac cctctaccag atccgcaata aacgcatgac ctggcgcact ttgctgacgc
    35761 tgctgtcggt gcgtaacggc aaacgtctgg ctgatggtat tcgggggcgc taaagatgct
    35821 ggaagatctg cgcgccaaca gctggagttt acgcccgtgc tgcatggttc ttgcctatcg
    35881 cgttgctcat ttttgttcgg tgtggcgcaa aaagaacgtc ctcaacaatc tgtgggcggc
    35941 cccgctgctg gtgctgtatc gcattatcac cgaatgcttt ttcggttatg aaatccaggc
    36001 agccgcaacc attggccgcc gctttactat ccatcacggt tacgccgtag tgatcaataa
    36061 aaacgtggtt gcgggggatg atttcaccat tcgccacggc gtcactatcg gcaatcgggg
    36121 tgccgataac atggcatgtc cacacattgg caacggcgtc gaactcggtg ccaacgtcat
    36181 tattcttggt gatatcacgc ttggtgacaa cgtcaccgtg ggcgcgggca gcgtggtgct
    36241 cgactctgtc ccggacaacg cgctggtggt gggagaaaaa gcgcgagtga aggtaattaa
    36301 atgaatattt tgcaatttaa tgtgcgactg gcggaaggcg gggcagcagg tgtggcgtta
    36361 gatctccacc agcgtgcgct gcaacagggg ctggcgtcac attttgtcta cggttacggc
    36421 aaaggcggca aagagagcgt cagccatcag aactatccgc aggtcatcaa acatacgccg
    36481 cggatgaccg cgatggcgaa tattgccctg tttcgtctgt ttaatcgcga tctgtttggc
    36541 aatttcaatg agttatatcg caccattact cgcacaccgg gtccggtggt cctgcatttt
    36601 catgtgctgc acagctactg gctaaatctt aagagcgtgg tgcgcttttg cgaaaaagtg
    36661 aaaaaccaca aaccggatat cactctggtc tggacgctgc acgatcactg gagcgttacc
    36721 ggacgctgcg cctttaccga cggttgcgaa ggctggaaaa cgggctgcca gaaatgcccg
    36781 accttaaata actatccgcc ggtgaagatt gatcgcgcgc atcagctggt ggcgggcaaa
    36841 cgccagttat tccgcgagat gctggcgctg ggctgtcagt ttatttcccc cagccagcat
    36901 gtggctgacg ctttcaatag tctgtacggg ccagggcgtt gccggattat caataatggc
    36961 attgatatgg caaccgaagc gattctggca gatctgcctc cggtgcgtga aacccagggc
    37021 aagccgaaaa tcgcggtggt ggcgcatgac ttgcgttacg acggcaagac taaccagcaa
    37081 ctggtgcgtg agatgatggc gctgggcgac aaaatcgaac tgcatacctt tggtaagttc
    37141 tcgccgttca ccgctggcaa cgtggttaat cacggctttg aaactgacaa gcgcaagttg
    37201 atgagcgcgc tcaatcagat ggatgcgctg gtattcagtt ctcgcgtcga taactacccg
    37261 ctgattttgt gtgaggcgtt atcaattggc gtgccggtga ttgccaccca tagcgacgcg
    37321 gcacgggaag tgttgcaaaa atccggcggt aaaaccgtcg acgaagaaga ggtgctgcaa
    37381 ctggtccagt taagcaaacc ggaaatcgcg caggcgatat ttggtaccac gctggctgag
    37441 ttcagccaac gcagccgcgc cacctacagt ggacaacaga tgctggagga gtatgtcaac
    37501 ttctatcaga atctgtagct acctgctgct gccgctgatc tacctgctgg ttaacgtcaa
    37561 aatcgcccag cttggcgaaa gtttccccat cactatcgtc acttttttac ctgtcttgtt
    37621 actgctgttt ttagaacgca tcagcgttaa aaaattgatg attgccttag ggattggtgc
    37681 gggactcacg gcgttcaact atctgtttgg tcagtcgttg gatgccagta aatacgtcac
    37741 atcaaccatg ctgtttgtct atattgtgat cattattggc atggtgtgga gtattcgctt
    37801 taaaacgatt tcgccacaca accatcgcaa gatattacgt ttcttttatc tggtggtcgg
    37861 gctggtggtg gcgctggcgg cggtggagat ggcgcaaatt atcctcaccg gtggcagcag
    37921 tattatggag tcgatttcga aatatctgat ttacagcaac agctatgtac tgaatttcat
    37981 taaattcggc ggcaagcgca cgacagcact ttatttcgaa ccggcatttt tcgctctggc
    38041 attaatctca atttggctca gcatcaaaca gtttggtatc aaaacgccta aaaccgatgc
    38101 tatgattctc gcagggataa tattatccgg atcgttttca ggggttatga cctttatcct
    38161 gttttatttg ctggagtggg catttcaata tctgaataaa gaggcgatta agaaaaagtt
    38221 accgttggca ttgatttctc tggctgtatt cctggttggt gtggtaatcg cgtttcctta
    38281 tatttccacc cgtctgggcg atttaggtac ggaaggatcg tcatcgtatt atcgtattgt
    38341 cggtccgctg gtgatggtcg gttattcttt gacccatatt gacggtgtag tcagatttgg
    38401 ctcactttat gaatacgtcg catcattcgg aatatttaac ggtgcggatg tcggaaaaac
    38461 catagacaat ggtttgtatc tgctgattat ttatttttcc tggtccgcag tgtttttatc
    38521 gctgtggtac atggggaaag tgataaaaat gatgatcaac gcttttggtg ataaccgcaa
    38581 ttttcgcgtg caattatatc tttttactcc ggtatcgctg ttttttaccg gttcgatatt
    38641 tagcccggaa tatgcatttt taatcgtctg tccgtttatt ttgcgaaaag cgttaaatat
    38701 tacgaggtaa gaataagaac atgttgctta gcataatcac tgtcgcgttt cgtaacctcg
    38761 aagggatagt caaaacacat gcctcgctgg cgcatctggc gcaggcggaa gatatcagct
    38821 tcgaatggat tgttgtcgat ggcggttcca atgacggcac ccgtgagtat ctggaaaatc
    38881 tcaatggtat ctataaccta cgctttgtca gcgagccaga taacggtatc tacgacgcca
    38941 tgaacaaagg cattacgatg gcacaaggca agttcgcgtt gtttctcaat tcgggcgata
    39001 tttttcatca ggatgccgca aattttgtcc gtaagttaaa aatgcaaaaa gataacatga
    39061 tgatcaccgg cgatgcgctg ctggattttg gcgacgggca taaaattaaa cgtagcgcca
    39121 aaccgggctg gtatatttat cacagcctgc ccgccagtca tcaggcgata tttttccctg
    39181 tatccggctt gaaaaaatgg cgttatgacc tggaatataa agtttcttcc gactacgcgc
    39241 tggcagccaa aatgtataaa gccggttatg catttaaaaa actcaatggc ctggtgtctg
    39301 aattttccat gggtggggta tctaccacca ataatatgga attgtgtgct gacgcgaaaa
    39361 aagtccaacg acaaatatta catgtacctg gcttttgggc tgaattatcc tggcatttac
    39421 gccaacgtac tacctcaaag acgaaagcct tatataacaa agtctgaata taaggagaac
    39481 caatgcaaga tttaagcggt ttctcggtgc cgaaagggtt ccggggcggc aacgctatta
    39541 aagtgcaatt atggtgggca gtacaggcaa caatatttgc ctggtcgcca caagtattgt
    39601 atcgctggcg ggcattttta ttacgtttat tcggcgcaaa aataggaaaa aacgtagtta
    39661 ttcgtccgtc agtaaaaatt acctatccgt ggaaattaac cttaggtgat tacgcgtggg
    39721 tcggcgatga cgtcaattta tataccctcg gtgaaataac cattggcgca cattcggtga
    39781 tatcgcaaaa aagttattta tgcaccggta gccacgacca tgcaagtcaa catttcacca
    39841 ttaatgctac gcctattgtg attggcgaga aatgctggct ggcgacagat gtttttgttg
    39901 cccctggcgt cacgattggc gacggcaccg tcgtgggtgc acgaagcagt gtttttaaat
    39961 cgcttccggc aaatgtggtt tgccggggga atcccgcagt ggtgatacgc gaacgcgttg
    40021 aaactgaata aattcaaaaa atacagagga ataagacatg tcaaaagtcg ctctcatcac
    40081 cggtgtaacc ggacaagacg gttcttacct ggcagagttt ctgctggaaa aaggttacga
    40141 ggtgcatggt attaagcgtc gcgcatcgtc attcaacacc gagcgcgtgg atcacattta
    40201 tcaggatccg cacacctgca acccgaaatt ccatctgcat tatggcgacc tgagtgatac
    40261 ctccaacctg acgcgcattt tgcgtgaagt gcagccggat gaagtgtaca acctgggcgc
    40321 aatgagccac gttgcggttt cttttgagtc accggaatat accgcagacg tcgatgcgat
    40381 gggtacgctg cgcctgctgg aggcgatccg cttcctcggt ctggaaaaga aaacccgttt
    40441 ctatcaggct tccacctctg aactgtatgg tctggtgcag gaaattccgc agaaagaaac
    40501 cacgccgttc tacccgcgat ctccgtatgc agtcgccaaa ctgtacgcct actggatcac
    40561 cgttaactac cgcgaatcct acggcatgta cgcctgtaac ggtattctct tcaaccatga
    40621 atccccgcgc cgcggcgaaa ctttcgttac ccgcaaaatc acccgcgcaa tcgccaacat
    40681 cgcccagggg ctggagtcgt gcctgtacct cggcaatatg gattccctgc gtgactgggg
    40741 ccatgccaaa gactacgtaa aaatgcagtg gatgatgctg caacaggaac aaccggaaga
    40801 tttcgtaatt gctaccggcg ttcagtactc cgtgcgtcag ttcgtggaaa tggcggcagc
    40861 acagctgggc atcaaactgc gctttgaagg cacgggcgtt gaagagaagg gcattgtggt
    40921 ttccgtcacc gggcatgacg cgccaggtgt taaaccgggt gatgtgatta tcgccgttga
    40981 tccgcgttac ttccgtccgg ctgaagttga aacgctgctc ggtgacccga ccaaagcgca
    41041 cgaaaaactg ggctggaaac cggaaatcac cctcagagag atggtgtctg aaatggtggc
    41101 taatgacctt gaagcagcga aaaaacactc tctgctgaaa tctcacggct acgacgtggc
    41161 gatcgcgctg gagtcataag catgagtaaa caacgcattt ttattgctgg ccatcgcggg
    41221 atggtcggtt ccgccatcag gcggcagctc gaacagcgcg gtgatgtgga gctggtatta
    41281 cgcacccgcg acgagctgaa cctgctggac agccgcgccg tgcatgattt ctttgccagc
    41341 gaacgtattg accaggtcta tctggcggcg gcgaaagtgg gcggcattgt tgccaacaac
    41401 acctatccgg cggatttcat ctaccagaac atgatgattg agagcaacat cattcacgcc
    41461 gcgcatcaga acgacgtgaa caaactgctg tttctcggat cgtcctgtat ctacccgaaa
    41521 ctggcaaaac agccgatggc agaaagcgag ctgttgcagg gcacgctgga gccgaccaac
    41581 gagccttatg ccattgccaa aatcgccggg atcaaactgt gcgaatctta taaccgtcag
    41641 tacggacgcg attaccgctc ggtcatgccg accaacctgt acgggccgca cgacaacttc
    41701 catccgagta actcgcatgt gatcccagca ttgctgcgtc gcttccacga ggcgacggca
    41761 cagaatgcgc cggacgtggt ggtatggggc agcggtacgc cgatgcgtga atttctgcac
    41821 gtcgatgata tggcggcggc gagcattcat gtgatggagc tggcgcacga agtctggctg
    41881 gagaacaccc agccgatgct gtcgcacatt aacgtcggca cgggcgttga ctgcactatc
    41941 cgcgagctgg cgcaaaccat cgccaaagtg gtgggttaca aaggtcgggt ggtttttgat
    42001 gccagcaaac cggatggcac gccgcgcaaa ctgctggatg tgacgcgcct gcatcagctt
    42061 ggctggtatc acgaaatctc actggaagcg gggcttgcca gcacttacca gtggttcctt
    42121 gagaatcaag accgctttcg ggggtaatga tgtttttacg tcaggaagac tttgccacgg
    42181 tagtgcgctc cacaccgctt gtctctctcg actttattgt cgagaacagt cgcggcgagt
    42241 ttctgcttgg caaaagaacc aaccgcccgg cgcagggtta ctggtttgtg ccgggagggc
    42301 gcgtgcagaa agacgaaacg ctggaagccg catttgagcg gctgacgatg gcggaactgg
    42361 ggctgcgtct gccgataaca gcaggccagt tttacggtgt ctggcagcac ttttatgacg
    42421 ataacttctc cggcacggat ttcaccactc actatgtggt gctcggtttt cgcttcagag
    42481 tcgctgaaga agatctgcta ctgccggatg agcagcatga cgattaccgc tggctgacgc
    42541 cggacgcgct gctcgccagt aacgatgttc acgctaacag ccgcgcctat tttctcgctg
    42601 agaagcgagc cggagtaccc ggattatgaa aatactggtc tacggcatta actactcgcc
    42661 ggagttaacg ggcatcggca aatacaccgg agagatggtg gaatggcttg cggcacaagg
    42721 tcatgaggtg cgggtcatta ccgcaccgcc ttactacccg caatggcagg tgggtgagaa
    42781 ctattccgcc tggcgctaca aacgagaaga gggggccgtc acggtgtggc gctgcccgct
    42841 gtacgtgcca aaacagccga gcaccctgaa acgcttgttg catctcggca gttttgccgt
    42901 cagcagtttc tttccactga tggcgcaacg tcgctggaag ccggatcgca ttattggcgt
    42961 agtgccaacg ctgttttgca cgccgggaat gcgcctgctg gcgaaactct ctggtgcgcg
    43021 taccgtgctg catattcagg attacgaagt ggatgccatg ctggggctgg gccttgccgg
    43081 aaaaggcaaa ggcggcaaag tggcacagct ggcgacggcg ttcgaacgta gcggactaca
    43141 taacgtcgat aacgtctcca cgatttcgcg ttcgatgatg aataaagcca tcgaaaaagg
    43201 cgtggcggcg gaaaacgtca ttttcttccc aaactggtcg gaaattgccc gttttcagca
    43261 tgtcgcagag gccgatgttg atgcccttcg taaccagctt ggcctgccgg ataacaaaaa
    43321 aatcattctt tactccggca atattggtga aaagcagggg ctggaaaacg ttattgaagc
    43381 tgccgatcgt ctgcgcgatg aaccgctgat ttttgccatt gtcgggcagg gcggcggcaa
    43441 agcgcggctg gaaaaaatgg cgcagcagcg tggactgcgc aacatgcaat ttttcccgct
    43501 gcaatcgtat gacgctttac ccgcactgct gaagatgggc gattgccatc tggtggtgca
    43561 aaaacgcggc gcggcagatg ccgtattgcc gtcgaaactg accaatattc tggcggtagg
    43621 cggtaacgcg gtgattaccg cagaagccca caccgaactg gggcagcttt gcgaaacctt
    43681 tccaggcatt gcggtttgcg tagaaccgga atcggtcgag gcgctggtgg cggggatttg
    43741 ccaggcgctc ctgctgccca aacacaacac ggtggcacgt gaatatgccg aacgcacgct
    43801 cgataaagag aacgtgttac gtcaatttat aaatgatatt cggggataat tatggcgcag
    43861 tcgaaactct atccagttgt gatggcaggt ggctccggta gccgcttatg gccgctttcc
    43921 cgcgtacttt accccaagca gtttttatgc ctgaaaggcg atctcaccat gctgcaaacc
    43981 accatctgcc gcctgaacgg cgtggagtgc gaaagcccgg tggtgatttg caatgagcag
    44041 caccgcttta ttgtcgcgga acagctgcgt caactgaaca aactcaccga gaacattatt
    44101 ctcgaaccgg cagggcgtaa cactgcaccg gccattgcgc tggcggcgct ggcggcaaaa
    44161 cgtcatagcc cggagagcga cccgttaatg ctggtattgg cggcggatca tgtgattgcc
    44221 gatgaagacg cgttccgtgc cgccgtgcgt aatgccatgc cgtatgccga agcgggcaaa
    44281 ctggtgacct tcggcattgt gccggatcta cctgaaaccg gatatggcta tatccgtcgc
    44341 ggtgaagtat ctgcgggtga gcaggatacg gtggcctttg aagtggcgca gtttgtcgaa
    44401 aaaccgaatc tggaaaccgc tcaggcctat gtggcaagcg gcgaatatta ctggaacagc
    44461 ggtatgttcc tgttccgcgc cggacgctat ctcgaagaac tgaaaaaata tcgtccggat
    44521 attctcgatg cctgtgaaaa agcgatgagc gccgtcgatc cggatctcga ttttattcgt
    44581 gtggatgaag aagcgtttct cgcctgcccg gaagagtcgg tggattacgc ggtcatggaa
    44641 cgtacggcag atgccgttgt ggtgccgatg gatgcgggct ggagcgatgt cggctcctgg
    44701 tcctcattat gggaaattag cgcccacacc gccgagggca acgtttgcca cggcgatgtg
    44761 attaatcaca aaactgaaaa cagctatgtg tacgccgaat ctggcctcgt aaccaccgtc
    44821 ggggtgaaag atctggtagt ggtgcagact aaagatgcgg tgctgattgc cgaccgtaac
    44881 gcggtacagg atgtgaaaaa agtggtcgag cagatcaaag ccgatggtcg ccatgagcat
    44941 cgggtgcatc gcgaagtgta tcgtccgtgg ggcaaatatg actctatcga cgcgggcgac
    45001 cgctaccagg tgaaacgcat caccgtgaaa ccgggcgagg gtttgtcggt acagatgcac
    45061 catcaccgcg cggaacactg ggtagtggtc gcgggaacgg caaaagtcac cattgatggt
    45121 gatatcaaac tgcttggtga aaacgagtcc atttatattc cgctgggggc gacgcactgc
    45181 ctggaaaacc cggggaaaat tccgctcgat ttaattgaag tgcgctccgg ctcttatctc
    45241 gaagaggatg atgtggtgcg cttcgcggat cgctacggac gggtgtaaac gtcgcatcag
    45301 gcgctgtttg tcggatgcgg cgtgaacgcc ttatccgacc tacggttcgg gtttgtagtc
    45361 ctgataagac gcggcagcgt cgcatcaggc aatgaatgcg aaaccgagat gtaaataacg
    45421 acaaaaacaa aattggccgt ttcggtcagg gccaactatt gcctgaaaaa gggtaacgac
    45481 atgaaaaaat taacctgctt taaagcctat gatattcgcg ggaaattagg cgaagaactg
    45541 aatgaagata tcgcctggcg cattggtcgc gcctatggcg aatttctcaa accgaaaacc
    45601 attgtgttag gcggtgatgt ccgtctcacc agcgaaacct taaaactggc gctggcaaaa
    45661 ggtttacagg atgcgggcgt tgacgtgctg gatattggta tgtccggcac cgaagagatc
    45721 tatttcgcca cgttccatct cggcgtggat ggcggcattg aagttaccgc cagccataat
    45781 ccgatggatt ataacggcat gaagctggtt cgcgaggggg ctcgcccgat cagcggagat
    45841 accggactgc gcgacgtcca gcgtctggct gaagccaacg actttcctcc cgtcgatgaa
    45901 accaaacgcg gtcgctatca gcaaatcaac ctgcgtgacg cttacgttga tcacctgttc
    45961 ggttatatca atgtcaaaaa cctcacgccg ctcaagctgg tgattaactc cgggaatggc
    46021 gcagcgggtc cggtggtgga cgccattgaa gcccgcttta aagcccttgg cgcacccgtg
    46081 gaattaatca aagtgcacaa cacgccgaac ggcaatttcc ccaacggtat tcctaacccg
    46141 ctactgccgg aatgccgcga cgacacccgc aatgcggtca tcaaacacgg cgcggatatg
    46201 ggcattgcct ttgatggcga ttttgaccgc tgcttcctgt ttgacgaaaa agggcagttt
    46261 atcgaaggct actacattgt cggcctgctg gcagaagcgt tcctcgaaaa aaatcccagc
    46321 gcgaagatca tccacgatcc acgtctctcc tggaataccg ttgatgtggt gactgccgct
    46381 ggcggcacgc cggtgatgtc gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag
    46441 gaagacgcca tctacggtgg cgaaatgagc gcccaccatt acttccgtga tttcgcttac
    46501 tgcgacagcg gcatgatccc gtggctgctg gtcgccgaac tggtgtgcct gaaagataaa
    46561 acgctgggcg aactggtacg cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac
    46621 agcaaactgg cgcaacccgt tgaggcgatt aaccgcgtgg aacagcattt tagccgtgag
    46681 gcgctggcgg tggatcgcac cgatggcatc agcatgacct ttgccgactg gcgctttaac
    46741 ctgcgctcct ccaacaccga accggtggtg cggttgaatg tggaatcgcg cggtgatgtg
    46801 ccgctgatgg aagcgcgaac gcgaactctg ctggcgttgc tgaacgagta atgtcggatc
    46861 ttcccttacc ccattgcggg taaggggcta ataacaggaa caacgatgac aaatctaaaa
    46921 aagcgcgagc gagcgaaaac caatgcatcg ttaatctcta tggtgcaacg cttttcagat
    46981 atcaccatca tgtttgccgg actatggctg gtttgcgaag tgagcggact gtcattcctc
    47041 tacatgcacc tgttggtggc gctgattacg ctggtggtgt tccagatgct gggcggcatc
    47101 accgattttt atcgctcatg gcgcggtgtt cgggcagcga cagaatttgc cctgttgcta
    47161 caaaactgga ccttaagcgt gattttcagc gccggactgg tggcgttcaa caatgatttc
    47221 gacacgcaac tgaaaatctg gctggcgtgg tatgggctga ccagcatcgg actggtggtt
    47281 tgccgttcat gtattcgcat tggggcgggc tggctgcgta atcatggcta taacaagcgc
    47341 atggtcgcgg tggcggggga tttagccgcc ggacaaatgc tgatggagag cttccgtaat
    47401 cagccgtggt tagggtttga agtggtgggt atataccacg acccgaaacc gggcggcgtt
    47461 tctaacgact gggcgggtaa cttgcaacag ctggtcgagg atgcaaaagc gggaaagatt
    47521 cataacgtct atatcgcgat gcaaatgtgc gacggcgcgc gagtgaaaaa actggtccat
    47581 caattggcgg acaccacctg ttcggtgctg ctgatccccg atgtctttac cttcaacatt
    47641 ctccattcac gtctcgaaga gatgaacggc gttccggtgg tgccgcttta cgatacgccg
    47701 ctttccgggg ttaatcgcct gataaaacgt gcggaagaca ttgtgctggc gacgcttatt
    47761 ctgctgctga tctccccggt gctgtgctgt attgcgctgg cggtgaaact gagttcacca
    47821 gggccggtta ttttccgcca gactcgctac ggcatggatg gcaagccgat caaagtgtgg
    47881 aagttccgtt ccatgaaagt gatggagaac gacaaagtgg tgactcaggc gacgcagaac
    47941 gatccgcgcg tcactaaagt ggggaacttt ctgcgccgca cctcgctgga tgaactgccg
    48001 cagtttatca atgtgctgac cggcgggatg tcgattgtcg gtccgcgtcc gcacgcggta
    48061 gcgcataacg aacagtatcg ccagctcatt gaaggctaca tgctgcgcca caaagtgaaa
    48121 ccgggcatta ccggctgggc gcagattaat ggctggcgcg gcgaaaccga cacactggag
    48181 aaaatggaaa aacgcgtcga gttcgacctt gagtacatcc gcgaatggag cgtctggttc
    48241 gatatcaaaa tcgttttcct gacggtattc aaaggcttcg ttaacaaagc ggcatattga
    48301 tatgagctta cgtgaaaaaa ccatcagcgg cgcgaagtgg tcggcgattg ccacggtgat
    48361 catcatcggc ctcgggctgg tgcagatgac cgtgctggcg cggattatcg acaaccacca
    48421 gttcggcctg cttaccgtgt cgctggtgat catcgcgctg gcagatacgc tttctgactt
    48481 tggtatcgct aactcgatta ttcagcgaaa agaaatcagc caccttgaac tcaccacgtt
    48541 gtactggctg aacgtcgggc tggggcttgt ggtgtgcgtg gcggtgtttt tgttgagtga
    48601 tctcatcggc gacgtgctga acaacccgga cctggcaccg ttgattaaaa cattgtcgct
    48661 ggcgtttgtg gtgatccccc acgggcagca gttccgcgcg ttgatgcaga aggagctgga
    48721 gttcaacaaa atcggcatga tcgaaaccag cgcggtgctg gcgggcttta cttttacggt
    48781 ggttagcgcc catttctggc cgctggcgat gaccgcgatc ctcggttatc tggtcaatag
    48841 tgcggtaaga acgctgctgt ttggctactt tggccgcaaa atttatcgcc ccggtctgca
    48901 tttctcgctg gcgtcagttg cgccgaactt acgctttggt gcctggctga cggcggacag
    48961 catcatcaac tatctcaaca ccaacctttc aacgctggtg ctggcgcgta ttcttggcgc
    49021 gggcgtggca gggggataca acctggcgta caacgtggcc gttgtgccac cgatgaagct
    49081 gaacccaatc atcacccgcg tgttgtttcc ggcattcgcc aaaattcagg acgataccga
    49141 aaagctgcgt gttaacttct acaagctgct gtcggtagtg gggattatca actttccggc
    49201 gctgctgggg ctgatggtgg tgtcgaataa ctttgtaccg ctggtctttg gtgagaagtg
    49261 gaacagcatt attccggtgc tgcaattgct gtgtgtggtg ggtctgctgc gctccgtggg
    49321 taacccgatt ggttcgctgc tgatggcgaa agcgcgggtc gatatcagct ttaaattcaa
    49381 cgtattcaaa acatttctgt ttattccggc gattgttatt ggtgggcaga tggcgggcgc
    49441 aatcggcgtc acgcttggct tcctgctggt gcagattatc aacaccattc tgagttactt
    49501 cgtgatgatt aaaccggttc ttggttccag ttatcgccag tacatcctga gtttatggct
    49561 gccgttttat ctctcgctgc cgacgctggt ggtcagttat gcgctgggca ttgtactgaa
    49621 agggcaactg gcgctgggga tgctgctggc ggtgcaaata gccgcgggcg tgctggcgtt
    49681 tgtggtgatg attgtgctgt cgcgccatcc gctggtggtg gaagtgaagc gtcagttttg
    49741 tcgcagcgaa aaaatgaaaa tgcttttacg ggcggggtga atggttgttt taggctacgg
    49801 caataaaaac aacaacataa cctcacctgg catcaaattt atgaggaaaa aatgaaatta
    49861 cttattctgg gtaaccacac ttgcggcaac cgcggcgaca gcgccatctt acgcggctta
    49921 cttgatgcca tcaacatcct caatccacac gccgaagtgg acgtaatgag ccgctatccg
    49981 gtcagttctt cctggctgct caaccgcccg gtgatgggcg atccgctgta cctgcaaatg
    50041 aaacaacaca acagcgcggc gggcgttgtc gggcgcgtta aaaaagtcct ccgtcgccgc
    50101 tatcagcacc aggtattgct ctcacgcgtc accgacactg gcaagctgcg taatatcgcg
    50161 atcgcccagg gattcactga cttcgtgcgc ctgctgtcag gttacgacgc cattattcag
    50221 gtcggcggat cgttttttgt cgatctctat ggcgtgccgc agtttgagca cgcgctttgc
    50281 acatttatgg cgaaaaagcc gctgtttatg attggtcaca gcgtcggccc cttccaggat
    50341 gagcaattta accaactggc gaactacgtt tttggtcact gtgacgcgct gatcctgcgc
    50401 gagtcggtta gcctcgatct aatgaaacgc agcaatatca ccactgcaaa agtggaacat
    50461 ggcgtcgata ccgcgtggct ggtcgatcac cacaccgaag atttcaccgc cagctatgcc
    50521 gttcaacact ggctggtcgt tgccgcacaa cagaaaacgg tagcaattac cctgcgcgaa
    50581 ctggcaccgt tcgacaaacg tctcggcacc actcaacatg cgtatgaaaa agcctttgcc
    50641 ggggtggtca atcgcattct cgatgaaggc tatcaggtga ttgcgctctc cacctgtacg
    50701 ggcatcgaca gctataacaa agacgaccgc atggtggcgc tcaatcttcg ccagcacatc
    50761 agcgatcctg cccgttacca tgtggtgatg gatgaactca acgatctgga aatgggcaaa
    50821 attctcggag cctgtgaact caccgtcggt acgcgcctgc actctgccat tatctcgatg
    50881 aatttctcca ctccggcaat tgccatcaac tacgaacata aatccgccgg gattatgcag
    50941 cagttgggac taccagagat ggcgattgat atccgtcatt tactggacgg cagcctgcaa
    51001 gcgatggttg cggatacctt aggccagctt ccggcgctga atgcacaact taacgaagcc
    51061 gtcagtcgcg aacgtcaggc aggaatgcag atggtgcaat ctgtgcttga acgcatcggg
    51121 gaggtgaaat gaaggtcggc ttctttttac tgaaatttcc gctgtcgtcg gaaaccttcg
    51181 tcctcaatca aattaccgcg tttattgata tgggatttga ggtggagatt ctcgcgctgc
    51241 aaaaaggcga cacccagaac acccacgcgg catggacgaa atacaacctt gccgccagaa
    51301 cccgctggtt acaggacgaa cctacgggca aagtggcgaa actgcgccac cgcgccagcc
    51361 agaccttacg cggcattcat cgtaaaaata cctggcaggc gctcaacctc aaacgctatg
    51421 gtgctgagtc gcgaaacctg attttgtctg ccatttgcgg ccaggtcgca acaccgtttc
    51481 atgccgatgt ctttatcgct cattttggcc cggcgggggt aaccgcagca aaactacgcg
    51541 aactgggtgt cattcgcggc aaaattgcca ccatcttcca cggtattgat atctccagtc
    51601 gggaagtgct caaccactac actcccgaat atcaacaact gtttcgccgt ggcgacctga
    51661 tgctaccaat aagcgatctg tgggccggaa ggctgcaaaa aatgggttgc cccagagaaa
    51721 aaatcgccgt atcgcgtatg ggcgtggaca tgacgcgctt tagcccgcgt ccggtgaaag
    51781 cgcccgcaac gccgctggaa atcatatctg tcgcacgctt aaccgagaaa aaaggcctgc
    51841 atgtggcgat tgaagcctgc cgtcagttga aagagcaggg cgtggcattt cgctatcgca
    51901 tcctcggcat tggcccgtgg gaacgacgcc tgcgtaccct catcgaacaa tatcaactgg
    51961 aagatgtggt agagatgccg ggctttaaac cgagccacga agtgaaagcg atgctcgacg
    52021 acgcggatgt cttcctcctg ccatcggtaa cgggcgcgga tggcgatatg gaaggtattc
    52081 cggtggcact aatggaagcg atggcggtcg gtatcccagt ggtttctact ctgcacagcg
    52141 gaataccgga actggtggag gccgataaat ccggctggct ggtgcctgag aacgatgctc
    52201 gcgcactggc gcaacgactg gcggcgttta gccaactgga caccgacgaa ctggctccgg
    52261 tcgtcaaacg cgcgcgcgaa aaagttgaac acgattttaa ccagcaggtg atcaatcgag
    52321 aactcgccag cttgctgcag gctttataga ggagaacgta tgccatttaa aaaactctcc
    52381 cgacgcacct tcctgacggc aagctccgcg cttgccttcc tccatacccc tttcgcccgc
    52441 gcacttcccg cccgacaaag cgttaacatt aacgactaca acccacacga ctggatcgcc
    52501 tcatttaaac aagccttcgg cgaagggcaa acggtcgtcg tgcctgccgg attggtttgc
    52561 gacaatatca acaccggcat cttcattcct gcaggtaaaa cgttacacat ccttggaagc
    52621 ctgcgcggca acggcagagg gcgatttgtc ttacaggacg gcagccaggt gacaggggag
    52681 gagggcggca gtatgcataa catcaccctg gatgtgcgcg gctctgactg caccatcaaa
    52741 gggctggcga tgagcggctt tggtccggta acgcagattt atatcggcgg caaaaacaaa
    52801 cgggtcatgc gcaacctgac catcgataac ctcactgtca gccacgctaa ttacgccatc
    52861 ttacgccagg gatttcataa ccagattatc ggtgccaaca tcaccaactg taaatttagt
    52921 gatttacagg gcgacgccat tgaatggaac gtggcgatta acgaccgtca tattttgatc
    52981 tccgaccatg tcatcgagcg catcaactgt accaacggca aaatcaactg gggaatcggc
    53041 ataggccttg cgggaagtac ctacgataat aagtacccgg aagaccaggc agtgaaaaac
    53101 tttgtcgtgg cgaatatcac gggatcggat tgtcggcagt tgatccatgt tgaaaatggt
    53161 aaacattttg ttattcgtaa tatcaaagcc cgcaatatca cgccggattt cagtaagaaa
    53221 gcaggcattg ataacgcgac agttgctatt tatggttgtg acaatttcgt gattgataat
    53281 attgaaatga ttaatagcgc cgggatgtta atcggctatg gggtaattaa aggcaaatat
    53341 ctctcgatac cgcaaaattt ccgagtgaat aatattcaac tggataacac ccatcttgct
    53401 tataaattgc gcggcatcca aatctccgct gggaatgccg tctcctttgt ggcgctgact
    53461 aacattgaga tgaagcgtgc gtcgctggag ttacacaaca aaccgcaaca cctttttatg
    53521 cgaaatatca aggtgatgca ggaatcctca gttggaccag cattgagcat gaactttgac
    53581 atgcgcaaag acgttcgcgg catttttatg gcgaaagaag aaacactgct gtcttttgca
    53641 aatgttcatg cggtgaatga aagagggcaa agttccgtcg atatcgacag gattaatcac
    53701 catattgtta atgtggaaaa gattaacttt agattgccgg aacggaggga gtagatttgc
    53761 gaccattcct ggaaaaatgg agtcatactt aggaacaatg ctactgcaat ccacaacgaa
    53821 gcggcgtaac atcacaagta attcagtaat caattcaggg taattgatgc tggcgaaaaa
    53881 aatcgaacaa gctataattc agcaaccatt ttacaggtgg atgaaataat gacgaattta
    53941 aaagcagtta ttcctgtagc gggtctaggg atgcatatgt tgcctgccac taaggcgatt
    54001 cccaaagaga tgctaccgat cgtcgacaag ccaatgattc agtacattgt tgacgagatt
    54061 gtggctgcag ggatcaaaga aatcctcctg gtaactcacg cgtccaagaa cgcggtcgaa
    54121 aaccacttcg acacctctta tgaattagaa tctctccttg aacagcgcgt gaagcgtcaa
    54181 ctgctggcgg aagtgcaatc tatctgcccg cctggcgtga ctattatgaa cgtgcgtcag
    54241 ggcgaacctt taggtttagg ccactccatt ttgtgtgcgc gacctgccat tggtgacaac
    54301 ccgtttgtcg tggtactgcc agacgttgtg atcgacgatg ccagcgccga cccgctacgt
    54361 tacaaccttg ctgccatgat tgcgcgcttc aacgaaacgg gccgcagcca ggtgctggca
    54421 aaacgtatgc cgggtgacct ctctgaatac tcggtcatcc agaccaaaga accgctggat
    54481 cgcgaaggta aagtcagccg cattgttgaa tttatcgaaa aaccggatca gccgcagacg
    54541 ctggactcag acatcatggc cgttggtcgc tatgtgcttt ctgctgatat ttggccggaa
    54601 cttgaacgca cgcagcctgg cgcatgggga cgtattcagc tgactgatgc cattgctgaa
    54661 ctggcgaaaa aacagtccgt tgatgccatg ctgatgaccg gcgacagcta cgactgcggt
    54721 aaaaaaatgg gctatatgca ggcgtttgtg aagtatggac tgcgtaacct aaaagaaggg
    54781 gcgaagttcc gtaaagggat tgagaagctg ttaagcgaat aatgaaaatc tgaccggatg
    54841 taacggttga taagaaaatt ataacggcag tgaagattcg tggcgaaagt aatttgctgc
    54901 gaatattcct gccgttgttt tatataaaca atcagaataa caacgagtta gcaataggat
    54961 tttagtcaaa gttttccagg attttccttg tttccagagc ggattggtaa gacaattagc
    55021 gtttgaattt ttcgggttta gcgcgagtgg gtaacgctcg tcacatcgta gacatgcatg
    55081 cagtgctctg gtagctgtaa agccaggggc ggtagcgtgc gttaatacct ctattaatca
    55141 aactaagagc cgctaatttc acagcatgct ctgaagtaat atggaataaa aaagtgaaga
    55201 tacttgttac tggtggcgca ggatttattg gttctgctgt agttcgtcac attataaata
    55261 atacgcagga tagtgttgtt aatgtcgata aattaacgta cgccggaaac ctggaatcac
    55321 ttgctgatgt ttctgattct gaacgctatg tttttgaaca tgcggatatt tgcgatgctg
    55381 ctgcaatggc gcggattttt gctcagcatc agccggatgc agtgatgcac ctggctgctg
    55441 aaagccatgt ggatcgttct atcactggcc ctgcggcatt tattgaaacc aatattgttg
    55501 gtacttatgt ccttttagaa gccgctcgca attactggtc tgctcttgat agcgacaaga
    55561 aaaatagttt ccgttttcat catatttcta ctgacgaagt atatggcgac ttaccccatc
    55621 ctgatgaagt aaatagtaat gaagcgttac cgctatttac ggaaatgaca gcttatgcac
    55681 caagtagccc atattctgct tctaaagcat ccagcgatca tttagtccgc gcgtggaaac
    55741 gtacctatgg tttaccgacc attgtgacta attgttctaa caattatggt ccttatcatt
    55801 tcccggaaaa actgattcca ttggttattc ttaatgctct ggaaggtaag gcattaccta
    55861 tttatggtaa gggggaccaa attcgcgact ggttgtatgt agaagatcat gcccgggcat
    55921 tgtacaccgt tatgacccag ggtgtagtag gtgaaaccta caatatcggt ggtcgtaacg
    55981 agaaaaaaaa cctggacgtg gtaaatacta tttgcgatct gcttgatgag attgtaccta
    56041 aacaagggtc ttatcgcgat caaattatct acgttactga ccgtcctggg cacgatcgcc
    56101 gctatgcgat tgatgcatca aaaatcagcg atgagctggg ctggaaaccg caggaaacct
    56161 ttgaatcggg aattcgaaaa actattggtt ggtatttaaa taatttagag tggtgccgtc
    56221 gtgtgcaaga cggtagctat caacgtgaac gtttaggatt acagtaaatg aaaggtatca
    56281 tacttgcagg tggttcagga actcgtttat atccagtcac aatggctgta agtaaacaat
    56341 tgttgccagt ttatgataaa ccaatgattt attacccatt aagtacatta atgttggctg
    56401 gtatacgaaa tatacttatt ataagtacac ctcaagatat tcctcgtttt atggggttat
    56461 taggtgatgg tagtcaatgg ggacttcagt tagaatacaa aatacagaat agtcctgatg
    56521 gattagcgca agcatttata ttaggtgagg attttattgg aaacgataat tgtgcattga
    56581 ttttagggga taatatattc tatggacatg atctccagaa acacctagaa atagcgctct
    56641 caaaagataa aggtgctaca gttttcgctt atcatgttaa agaccctatg agatacggcg
    56701 ttgtagagtt tgataaacaa ggtaaagcca tttcacttga agagaaacct gaaattccaa
    56761 aaagtaatta tgcagtaacg ggattgtatt tttatgataa taatgttgtt gagatagcga
    56821 aatcattaaa accctcgaag cgaggagagc tagaaataac ggatattaat cgactctatt
    56881 tggagcgggg tgagctttct gtagctatga tggggcgagg ttatgcttgg ctagatactg
    56941 gaactcatga aagtctaatt gaagctagca atttcatcca gacaattgaa gctaggcagg
    57001 gtttaa
//
LOCUS       DAZFYC010000017        55521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFYC010000017 DAZFYC010000000
VERSION     DAZFYC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.017"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            167..1531
                     /gene="yajR"
                     /locus_tag="ACHKW5_001187"
     CDS             167..1531
                     /gene="yajR"
                     /locus_tag="ACHKW5_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510620.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1659..2150)
                     /gene="yajQ"
                     /locus_tag="ACHKW5_001188"
     CDS             complement(1659..2150)
                     /gene="yajQ"
                     /locus_tag="ACHKW5_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO7510621.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2318..3229
                     /gene="panE"
                     /locus_tag="ACHKW5_001189"
     CDS             2318..3229
                     /gene="panE"
                     /locus_tag="ACHKW5_001189"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO7510622.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3192..3782
                     /gene="yajL"
                     /locus_tag="ACHKW5_001190"
     CDS             3192..3782
                     /gene="yajL"
                     /locus_tag="ACHKW5_001190"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO7510623.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3836..5284)
                     /gene="thiI"
                     /locus_tag="ACHKW5_001191"
     CDS             complement(3836..5284)
                     /gene="thiI"
                     /locus_tag="ACHKW5_001191"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO7510624.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5490..5732
                     /gene="xseB"
                     /locus_tag="ACHKW5_001192"
     CDS             5490..5732
                     /gene="xseB"
                     /locus_tag="ACHKW5_001192"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO7510625.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5732..6631
                     /gene="ispA"
                     /locus_tag="ACHKW5_001193"
     CDS             5732..6631
                     /gene="ispA"
                     /locus_tag="ACHKW5_001193"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO7510626.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6656..8518
                     /gene="dxs"
                     /locus_tag="ACHKW5_001194"
     CDS             6656..8518
                     /gene="dxs"
                     /locus_tag="ACHKW5_001194"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO7510627.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8698..9672
                     /gene="yajO"
                     /locus_tag="ACHKW5_001195"
     CDS             8698..9672
                     /gene="yajO"
                     /locus_tag="ACHKW5_001195"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO7510628.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9726..10244)
                     /gene="pgpA"
                     /locus_tag="ACHKW5_001196"
     CDS             complement(9726..10244)
                     /gene="pgpA"
                     /locus_tag="ACHKW5_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO7510629.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10222..11199)
                     /gene="thiL"
                     /locus_tag="ACHKW5_001197"
     CDS             complement(10222..11199)
                     /gene="thiL"
                     /locus_tag="ACHKW5_001197"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO7510630.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11277..11696)
                     /gene="nusB"
                     /locus_tag="ACHKW5_001198"
     CDS             complement(11277..11696)
                     /gene="nusB"
                     /locus_tag="ACHKW5_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO7510631.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11716..12186)
                     /gene="ribE"
                     /locus_tag="ACHKW5_001199"
     CDS             complement(11716..12186)
                     /gene="ribE"
                     /locus_tag="ACHKW5_001199"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO7510632.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12275..13378)
                     /gene="ribD"
                     /locus_tag="ACHKW5_001200"
     CDS             complement(12275..13378)
                     /gene="ribD"
                     /locus_tag="ACHKW5_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO7510633.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSTQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQVL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13382..13831)
                     /gene="nrdR"
                     /locus_tag="ACHKW5_001201"
     CDS             complement(13382..13831)
                     /gene="nrdR"
                     /locus_tag="ACHKW5_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO7510634.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13982..14521
                     /gene="yajI"
                     /locus_tag="ACHKW5_001202"
     CDS             13982..14521
                     /gene="yajI"
                     /locus_tag="ACHKW5_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7510635.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSALNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLKSQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14820..15704
                     /gene="tsx"
                     /locus_tag="ACHKW5_001203"
     CDS             14820..15704
                     /gene="tsx"
                     /locus_tag="ACHKW5_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO7510636.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15881..16228)
                     /gene="yajD"
                     /locus_tag="ACHKW5_001204"
     CDS             complement(15881..16228)
                     /gene="yajD"
                     /locus_tag="ACHKW5_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO7510637.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16357..17328)
                     /gene="secF"
                     /locus_tag="ACHKW5_001205"
     CDS             complement(16357..17328)
                     /gene="secF"
                     /locus_tag="ACHKW5_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO7510638.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17339..19186)
                     /gene="secD"
                     /locus_tag="ACHKW5_001206"
     CDS             complement(17339..19186)
                     /gene="secD"
                     /locus_tag="ACHKW5_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO7510639.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19214..19546)
                     /gene="yajC"
                     /locus_tag="ACHKW5_001207"
     CDS             complement(19214..19546)
                     /gene="yajC"
                     /locus_tag="ACHKW5_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO7510640.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19569..20696)
                     /gene="tgt"
                     /locus_tag="ACHKW5_001208"
     CDS             complement(19569..20696)
                     /gene="tgt"
                     /locus_tag="ACHKW5_001208"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO7510641.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20752..21822)
                     /gene="queA"
                     /locus_tag="ACHKW5_001209"
     CDS             complement(20752..21822)
                     /gene="queA"
                     /locus_tag="ACHKW5_001209"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO7510642.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21915..22496
                     /gene="acpH"
                     /locus_tag="ACHKW5_001210"
     CDS             21915..22496
                     /gene="acpH"
                     /locus_tag="ACHKW5_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO7510643.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22501..24318)
                     /gene="malZ"
                     /locus_tag="ACHKW5_001211"
     CDS             complement(22501..24318)
                     /gene="malZ"
                     /locus_tag="ACHKW5_001211"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO7510644.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(24474..25847)
                     /gene="proY"
                     /locus_tag="ACHKW5_001212"
     CDS             complement(24474..25847)
                     /gene="proY"
                     /locus_tag="ACHKW5_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO7510645.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25923..27242)
                     /gene="brnQ"
                     /locus_tag="ACHKW5_001213"
     CDS             complement(25923..27242)
                     /gene="brnQ"
                     /locus_tag="ACHKW5_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO7510646.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27649..28944)
                     /gene="phoR"
                     /locus_tag="ACHKW5_001214"
     CDS             complement(27649..28944)
                     /gene="phoR"
                     /locus_tag="ACHKW5_001214"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO7510647.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29002..29691)
                     /gene="phoB"
                     /locus_tag="ACHKW5_001215"
     CDS             complement(29002..29691)
                     /gene="phoB"
                     /locus_tag="ACHKW5_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO7510648.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29881..31083
                     /gene="sbcD"
                     /locus_tag="ACHKW5_001216"
     CDS             29881..31083
                     /gene="sbcD"
                     /locus_tag="ACHKW5_001216"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO7510649.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITADEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31080..34223
                     /gene="sbcC"
                     /locus_tag="ACHKW5_001217"
     CDS             31080..34223
                     /gene="sbcC"
                     /locus_tag="ACHKW5_001217"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO7510650.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34349..35533
                     /gene="araJ"
                     /locus_tag="ACHKW5_001218"
     CDS             34349..35533
                     /gene="araJ"
                     /locus_tag="ACHKW5_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO7510651.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35676..36584)
                     /gene="mak"
                     /locus_tag="ACHKW5_001219"
     CDS             complement(35676..36584)
                     /gene="mak"
                     /locus_tag="ACHKW5_001219"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO7510652.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36709..37620
                     /gene="rdgC"
                     /locus_tag="ACHKW5_001220"
     CDS             36709..37620
                     /gene="rdgC"
                     /locus_tag="ACHKW5_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO7510653.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37698..37781)
                     /locus_tag="ACHKW5_001221"
     CDS             complement(37698..37781)
                     /locus_tag="ACHKW5_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510654.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37778..38119)
                     /locus_tag="ACHKW5_001222"
                     /pseudo
     CDS             complement(37778..38119)
                     /locus_tag="ACHKW5_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38267..38551)
                     /gene="ppnP"
                     /locus_tag="ACHKW5_001223"
     CDS             complement(38267..38551)
                     /gene="ppnP"
                     /locus_tag="ACHKW5_001223"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO7510655.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38623..39300)
                     /gene="aroM"
                     /locus_tag="ACHKW5_001224"
     CDS             complement(38623..39300)
                     /gene="aroM"
                     /locus_tag="ACHKW5_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO7510656.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDIQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39558..39749)
                     /gene="yaiA"
                     /locus_tag="ACHKW5_001225"
     CDS             complement(39558..39749)
                     /gene="yaiA"
                     /locus_tag="ACHKW5_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO7510657.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39799..40323)
                     /gene="aroL"
                     /locus_tag="ACHKW5_001226"
     CDS             complement(39799..40323)
                     /gene="aroL"
                     /locus_tag="ACHKW5_001226"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO7510658.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40506..40964)
                     /gene="yaiI"
                     /locus_tag="ACHKW5_001227"
     CDS             complement(40506..40964)
                     /gene="yaiI"
                     /locus_tag="ACHKW5_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO7510659.1"
                     /translation="MTIWVDADACPSVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41084..41893
                     /gene="proC"
                     /locus_tag="ACHKW5_001228"
     CDS             41084..41893
                     /gene="proC"
                     /locus_tag="ACHKW5_001228"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO7510660.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41910..43025)
                     /gene="adrA"
                     /locus_tag="ACHKW5_001229"
                     /gene_synonym="dgcC"
     CDS             complement(41910..43025)
                     /gene="adrA"
                     /locus_tag="ACHKW5_001229"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO7510661.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43127..43447)
                     /gene="psiF"
                     /locus_tag="ACHKW5_001230"
     CDS             complement(43127..43447)
                     /gene="psiF"
                     /locus_tag="ACHKW5_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO7510662.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAEKTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43566..44981)
                     /gene="phoA"
                     /locus_tag="ACHKW5_001231"
     CDS             complement(43566..44981)
                     /gene="phoA"
                     /locus_tag="ACHKW5_001231"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO7510663.1"
                     /translation="MKQSTIALALLTLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDHTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45082..45342)
                     /gene="iraP"
                     /locus_tag="ACHKW5_001232"
     CDS             complement(45082..45342)
                     /gene="iraP"
                     /locus_tag="ACHKW5_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO7510664.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(45524..45727)
                     /locus_tag="ACHKW5_001233"
     CDS             complement(45524..45727)
                     /locus_tag="ACHKW5_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510665.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            45805..46899
                     /gene="ddlA"
                     /locus_tag="ACHKW5_001234"
     CDS             45805..46899
                     /gene="ddlA"
                     /locus_tag="ACHKW5_001234"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7510666.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(46923..47135)
                     /gene="yaiZ"
                     /locus_tag="ACHKW5_001235"
     CDS             complement(46923..47135)
                     /gene="yaiZ"
                     /locus_tag="ACHKW5_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO7510667.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            47395..47703
                     /gene="yaiY"
                     /locus_tag="ACHKW5_001236"
     CDS             47395..47703
                     /gene="yaiY"
                     /locus_tag="ACHKW5_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO7510668.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(47762..48856)
                     /gene="yaiW"
                     /locus_tag="ACHKW5_001237"
     CDS             complement(47762..48856)
                     /gene="yaiW"
                     /locus_tag="ACHKW5_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO7510669.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(48869..50089)
                     /gene="sbmA"
                     /locus_tag="ACHKW5_001238"
     CDS             complement(48869..50089)
                     /gene="sbmA"
                     /locus_tag="ACHKW5_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO7510670.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGEWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            50441..51598
                     /gene="ampH"
                     /locus_tag="ACHKW5_001239"
     CDS             50441..51598
                     /gene="ampH"
                     /locus_tag="ACHKW5_001239"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO7510671.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(51599..52222)
                     /gene="iprA"
                     /locus_tag="ACHKW5_001240"
     CDS             complement(51599..52222)
                     /gene="iprA"
                     /locus_tag="ACHKW5_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO7510672.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(52310..55288)
                     /locus_tag="ACHKW5_001241"
     CDS             complement(52310..55288)
                     /locus_tag="ACHKW5_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7510673.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHDFTDGTTN
                     QLNISNSLIHGSITSMLPMGESSDGHYVYQFSEYGTDRVIDDNWHDGDVFTLNIANST
                     IDDDYEGLYFTDSYLDGDVTKYTNETFHTPAGEGEEYAGLFANGGVGLGLAVNLDVES
                     NINISNNSRVAGISLTQGNTVNNTYTTESHTWDNNISVTDSTVTSGSVTTLEDSGFYG
                     NSAEPSDYSGNGEANDVALYFSDSAASNYSMKNNVYFSNSTLLGDVVFASTFNANFYP
                     QGHDSNADGVLDTNGGWADDSLNVDELNITLDNGSKWVGSATTSANVDDESTASTDWY
                     DVTGNSLYPGVVTEANAWGRTIDDQVFQSGVFNVTLNNGSEWNTVNASNIDTLAVNNG
                     SQVNVSESSLISDSIALTNGSSLNIGDEGYVGTDHLTVDSYSTVALSSDDNSSIYNGY
                     YTPSLLANTITVTNGGLLDINVDQFDDNPFQTDTLELTSGNIADHNGNVVSGVFDIHS
                     SDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVV
                     AATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNTNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
BASE COUNT        13876 a        14646 c        13781 g        13218 t
ORIGIN      
        1 gttttaaaat tcattttttc aacatataga gcattcattt tatgaatgca aaaaaacata
       61 cctgacacac aaattactct cctctgtaat attttctcat taacactctg tttacccaac
      121 atgcgctccg cactacacta tgtgggcatt tttttaaggg gtggtaatga acgattataa
      181 aatgacgcca ggtgagaggc gcgcgacctg gggtttaggg accgtattct cgttgcgcat
      241 gctgggcatg tttatggttc tgccggttct gaccacgtac ggcatggctc tgcaaggcgc
      301 cagcgaagca ttaatcggta ttgccattgg tatttatggt ctgactcagg ccgtttttca
      361 gattccgttt ggcctgcttt ccgaccgcat tggtcgcaaa ccattaattg tcggtgggct
      421 ggcggtgttt gccgccggta gcgttatcgc tgcgctttcc gactccatct ggggaattat
      481 tctgggccgg gcgctacaag gctctggagc aattgccgcc gccgttatgg cgctgctttc
      541 cgatctcacg cgcgaacaaa accgcaccaa agcgatggcg tttatcggcg tgagctttgg
      601 cattaccttt gccattgcga tggtgcttgg cccgatcatc actcacaaac ttgggctgca
      661 cgcgctgttc tggatgatcg ctattctggc aacaaccggc attgcgttga ccatttgggt
      721 tgtgcccaac agtagcactc acgtacttaa tcgtgagtcc ggaatggtga aaggcagttt
      781 cagtaaagtg ctggcggaac cgcggctgct aaaactcaac tttggcatta tgtgtctgca
      841 catgcttctg atgtctacgt ttgttgccct gcccggacag ctagctgatg cggggttccc
      901 ggcggctgaa cactggaagg tctatctggc gacgatgtta atcgcctttg gctcggtcgt
      961 gcctttcatt atctacgctg aagttaagcg caaaatgaag caagtctttg tcttctgcgt
     1021 cgggttgatc gtggttgcgg aaattgtgtt gtggaacgca caaacgcagt tctggcaact
     1081 ggtggtcggc gtgcagcttt tctttgtggc gtttaatttg atggaagccc ttctgccctc
     1141 acttatcagt aaagagtcgc cagcaggtta caaaggtacg gcgatgggtg tctactccac
     1201 cagccagttt cttggcgtgg cgattggcgg ttcgctgggc ggctggattg acggcatgtt
     1261 tgacggtcag ggggtatttc tcgctggcgc aatgctggcc gcagtgtggc tggcagtcgc
     1321 cagtaccatg aaagaaccgc cgtatgtcag cagtttgcgc attgaaatcc cggcggacat
     1381 tgccgcaaac gaagcgttaa aagtgcgttt gctggaaact gaaggcgtca aagaagtgtt
     1441 gattgcagaa gaagagcatt cagcttatgt gaaaatcgac agcaaagtga caaatcgctt
     1501 tgaggttgaa caggcaattc gtcaggcata aaaaacgcgg cgaattgcct gctgcgctac
     1561 gcttaccagg cctacaaagt tcttgagtta caaaccgttg taggccgaat caggcgttca
     1621 tgccgcatct gacatgaaca aaacgcacat tgtcgcgatt aatcgcggaa gtttttgaac
     1681 tggaacggct ggccgagatc gccaccacgt accatcgcca tgacagcctg caaatcatca
     1741 cgagatttgc cagtcacacg gatctcatcg ccctgaattt gcgcctgcac tttcagtttg
     1801 ctgtctttaa tcatcttgac gattttcttc tgggtcgcgc tctcaatgcc ctgtttcagt
     1861 ttcgcttcca caaaccaggt tttaccgcta tgaacgatat tttccggtac atccagtgaa
     1921 ctgccttcaa tgccgcgctt cagcagcttg gcacgcagaa tatccagcaa ctgattgacc
     1981 tggaagtcgg actcgctcaa cactttgatg gttttgctgg cgtcgttcag ctcaaatgag
     2041 gcttcaacgt tacggaagtc aaaacgggac tccacttcgc gactcgcgtt atcgaccgcg
     2101 ttacgtgctt cctgaagatc aacttcagag acaatatcga aagatggcat cttttcctct
     2161 cccttcattt ttgatgcgaa gcataatacc tgcaaagtta aataaccaac agtcctgcac
     2221 caggacgtat cccttaattg tgcatagtcg caacagcatg cacaattaag ggatagcctt
     2281 attgaacacc cggagtggtt gcgggtgagg aggaacaatg aaaattaccg tattgggatg
     2341 cggtgcctta gggcaattat ggcttacagc actttgtaaa cagggtcacg aagttcaggg
     2401 ctggctgcgc gtaccgcaac cttattgtag tgtgaatctg gttgagactg atggttcgat
     2461 atttaacgaa tcgctgaccg ccaacgatcc cgattttctt gccaccagcg atctgctcct
     2521 ggtgacgctg aaagcatggc aggtttacga tgccgtcaaa agcctcgcgt ccacactgcc
     2581 tgtaaccacg ccaatactgt taattcacaa cggcatgggc accatcgaag agttgcaaaa
     2641 cattcagcag ccattactga tgggcaccac cacccatgcc gcccgccgcg acggcaatgt
     2701 cattattcat gtggcaaacg gaatcacgca tattggtccg gcacggcaac aggatggcga
     2761 ttacagttat ctggcggata ttttgcaaac cgtgttgcct gacgttgcct ggcataacaa
     2821 tattcgcgcc gagctgtggc gcaagctggc cgtcaactgt gtgattaatc cactgaccgc
     2881 catctggaat tgcccgaatg gtgaattacg tcatcatccg caagaaatta tgcagatatg
     2941 cgaagaagtc gcggcagtga tcgagcgcga agggcatcat acttcagcag aagatttgcg
     3001 tgattacgtg atgcaggtga ttgatgccac agcggaaaat atctcgtcga tgttgcagga
     3061 tatccgcgcg ctgcgccaca ccgaaatcga ctatatcaat ggttttctct tacgccgcgc
     3121 ccgcgcgcat gggattgccg taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa
     3181 ggagagtgaa tatgagcgca tcggcactgg tttgcctcgc ccctggtagt gaagagactg
     3241 aagccgtcac cactatcgat ctgctggttc gcggcggtat caaagtcacc actgccagcg
     3301 tcgccagcga tggcaacctg gccattacct gctcgcgcgg cgtgaagctg ctggcggatg
     3361 cgccgctggt cgaggtggct gatggcgaat atgacgtgat cgtactgcct ggtggcatta
     3421 aaggcgcaga gtgttttcgc gatagtaccc tgctggttga aaccgttaaa cagttccacc
     3481 gttccgggcg tatcgtcgcg gctatttgcg ccgcgccagc caccgtgctg gtaccgcacg
     3541 atatcttccc gattggcaat atgaccggct tcccgacgct gaaagacaaa attcccgccg
     3601 aacaatggca ggacaagcgc gtcgtctggg atgcacgggt aaaattgctg accagccagg
     3661 ggccgggtac ggctattgat tttggtctga aaattatcga cctgctggtt gggcgcgaga
     3721 aagcccatga agtggcatca caactggtga tggcggcagg gatttataat tattacgagt
     3781 agtgtcggat gcggcaaacg tcgcatctga ccagatgcga cgtaaaaacc acaaattatg
     3841 ggcgatacac cttcacattg ttaaagccct gctcgcgcag atagagcgcc tgcagacggc
     3901 tcatcacccc gcgctcacac cacagtagcc aggttctgtt ctggtcgaga tcgccaaact
     3961 tggtgctcag tttatagaac ggcagagaaa ctacatcaat cccttcgact ttcagtggct
     4021 tatcttcctg ttcatcgata gaacggatat cgaggatcac gtcgttcggg ccgaagccat
     4081 tgacggtttc cacttccacc acttcctgct cggtctgctg ggcgatttcg cggatatcaa
     4141 cgttattcgc ttcttcaacc actttatcga gaatgctgaa gtcgaatttc tcttcttccg
     4201 cttcaatctt cgacttaacc gctttcaccg tcgggctttt ggagataaca ccgcaatatt
     4261 ccggcatcgt gcgagcaaag tcttcggtgc caatctggcg agccaggttg atgatgtgct
     4321 ctttgtcgta agaaatcagc ggacgcagga tcagcgtatc ggagacgtta tcaatcaggc
     4381 gcaggttggt cagcgtctga ctggacacct ggccgagcgc ttcgccggtg accagcgcct
     4441 gtacgccgta acgttcagcc actttagacg cggcacgcac catcatacgt ttgaggataa
     4501 cgcccatctg accgtcgtcg attttctcga gaatttcccc gacgaccggt tcgaaattaa
     4561 tagcgacaaa acgcacgcgg tgggagctgc caaaacggtt ccacagataa tgcgccacct
     4621 gacgcacgcc aatttcatgc gccgcgccgc caaggttaaa gaaacagtaa tgcacgcggc
     4681 agccgcgacg catcaacata taactggaaa caccggagtc gaaaccaccg gaaatgagcg
     4741 acagcacatc ttcctgggtg ccgatcggga aaccgccaat accttcgtag cggcctttaa
     4801 tcagcaggag acgatcgtct tccacttcca gatggacagt cacttccgga ttggtcagct
     4861 tcacgcgcgc ggactcaata tgctgattta aaccgccgcc gacgtaacgt tccacatcaa
     4921 tcgagctaaa atcatgtttg ccacgacgct tcacgcgtac gcagaaggtt ttgccttcca
     4981 gctgatcgcg atactgaacc aacgctttct caaaaatatc gtgcatgtcg gtaaacggca
     5041 cgtcttcgac ttcgagaata tggtggatac ccggaatacg ggtcagggcg tcgcgaatag
     5101 ccagacgctg gttttcatct tttgcgcgaa cttcgatgtt atcccagtgg cggacgacag
     5161 cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt cccggtaagg atttttataa
     5221 agcgcaagcg cacagattgg cttttgatgg tgatttccgg gaacaattta atgataaact
     5281 tcatggcggc aatggttcgt tggcaagcct taagcgactt gtatagggaa aaatacagca
     5341 gcccacacct gcggctgcat ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc
     5401 acatcacctt accattgcgc gttatttgct atttgccctg agtccgttac catgacgggg
     5461 cgaaaaatat tgagagtcag acattcatta tgccgaagaa aaatgaggct cccgccagct
     5521 ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc
     5581 cgctggaaga ggcgctgaac gagttcgaac gcggcgtgca gctggcacgt caggggcagg
     5641 ccaaattaca acaagccgaa cagcgcgtac aaattctgct gtctgacaat gaagacgcct
     5701 ctctaacccc ttttacaccg gacaatgagt aatggacttt ccgcagcaac tcgaagcctg
     5761 cgttaagcag gccaaccaag cgctgagccg ttttatcgcc ccactgccct ttcagaacac
     5821 tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt
     5881 tctggtttat gccaccggtc atatgtttgg cgttagcaca aacacgctgg acgcacccgc
     5941 tgccgccgta gagtgtatcc acgcttactc attaattcat gatgatttac cggcgatgga
     6001 tgatgacgat ctgcgtcgcg gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc
     6061 gattctcgct ggcgacgctt tacaaacgct ggcgttctcg attctaagcg atgccaatat
     6121 gccggaagtg tcggatcgcg acagaatttc gatgatttct gaactggcga gcgccagcgg
     6181 tattgccgga atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc
     6241 tctggacgcg cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt
     6301 tcgccttggt gcattaagcg ccggagataa agggcgtcgt gctctgccag tactcgacaa
     6361 gtacgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtagg
     6421 agatactgca acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta
     6481 ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc
     6541 ccgccagtcg ctgaaacaac tggctgaaca atcactcgat acctcggcac tggaagcgct
     6601 agcggactac atcatccagc gtaataaata aacaataagt attaataggc ccctgatgag
     6661 ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg agttacgact
     6721 gttgccgaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt tactcgacag
     6781 cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac tgaccgtggc
     6841 gctgcactat gtctacaaca ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc
     6901 ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg
     6961 cggcctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa gcgtcggtca
     7021 ttcatcaacc tccatcagtg ccggaattgg tattgcggtt gctgccgaga aagaaggcaa
     7081 aaatcgccgc accgtctgtg tcattggcga tggcgcgatt accgctggca tggcgtttga
     7141 agcgatgaat cacgcgggcg atatccgtcc tgatatgctg gtggtcctca acgacaatga
     7201 aatgtcgatt tccgaaaatg tcggcgcgct caataaccat ctggcacagc tgctttccgg
     7261 taagctttac tcttcgctgc gcgaaggcgg gaaaaaagtt ttctctggcg ttccgccaat
     7321 taaagagctg ctcaaacgta ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt
     7381 gtttgaagag ctgggcttta actacatcgg cccggttgac ggtcacgatg tgctggggct
     7441 tatcaccacg ctgaagaaca tgcgcgacct gaaaggcccg cagttcctgc atatcatgac
     7501 caaaaaaggt cgtggttatg aaccggcaga aaaagacccc atcactttcc acgccgtgcc
     7561 taaatttgat ccctccagcg gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc
     7621 aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat
     7681 tactccggcg atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat tcccggatcg
     7741 ttacttcgac gtggcaatcg ccgagcaaca cgcggtgacc tttgccgccg gtctggcgat
     7801 tggtgggtac aaacccattg tcgcgattta ctccactttc ctgcaacgcg cctatgatca
     7861 ggtgctgcat gacgtggcga ttcaaaagct cccggtcctg ttcgccatcg accgcgcggg
     7921 cattgttggt gctgacggtc aaacccatca gggcgctttt gacctctctt acctgcgctg
     7981 cataccggaa atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta
     8041 taccggctat cactataacg atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt
     8101 cggcgtggaa ctgacgccgc tggaaaaact accaattggc aaaggcattg tgaagcgtcg
     8161 tggcgagaaa ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc
     8221 cgaatcgctg aacgccacgc tggtcgatat gcgttttgtg aaaccgcttg atgaagcgtt
     8281 aattctggaa atggccgcca gccatgaagc gctggtcacc gtagaagaaa acgccattat
     8341 gggcggcgca ggcagcggcg tgaacgaagt gctaatggcc catcgtaaac cagtacccgt
     8401 gctgaacatt ggcctgcctg acttctttat tccgcaagga actcaggaag aaatgcgcgc
     8461 cgaactcggc ctcgatgccg ccggtatgga agccaaaatc aaggcctggc tggcataatc
     8521 cctactccac tcctgctatg cttaacaaat tattcataga ctctaaataa ttcgagttgc
     8581 aggaaggcgg caaacgagtg aagccccagg agcttacata agtaagtgac tggggtgaac
     8641 gaatgcagcc gcagcacatg caacttgaag tatgacgagt atggcaggag tggcagcatg
     8701 caatacaacc ccttaggaaa aaccgacctt cgcgtttccc gactttgcct cggctgtatg
     8761 acctttggcg agccagatcg tggtaatcac gcatggacac tgccggaaga gagtagccgt
     8821 cccatcatta aacgcgcgct ggaaggcggc ataaatttct ttgataccgc caatagctat
     8881 tccgatggca gcagcgaaga gatcgttggt cgcgcactgc gggatttcgc ccgtcgtgaa
     8941 gacgtggtcg ttgcgacgaa agtgttccat cgcgttggtg atttaccgga aggattatcc
     9001 cgtgcacaaa ttttgcgctc tatcgacgac agcctgcgcc gtctcggcat ggattatgtc
     9061 gatatcctgc aaattcatcg ctgggattac aacacgccga tcgaagagac gctggaagcc
     9121 ctgaacgacg tggtaaaagc cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct
     9181 tcgcagtttg ctcaggccct gaagctacaa aaacagcacg gctgggcgca gtttatcagt
     9241 atgcaggatc actacaatct gatttatcgc gaagaagagc gcgagatgct gcctctgtgt
     9301 tatcaggagg gcgtggcggt gattccgtgg agcccgctgg cgcgggggcg actgacgcgt
     9361 ccgtggggag aaactaccgc acgactggtg tctgatgagg tggggagaaa tctctataaa
     9421 gaaagcgatg aaaatgacgc gcagatcgca gagcggttaa cgggcgtcag tgaagaactc
     9481 ggtgcaacac gagcacaagt tgcgctggcc tggttgttga gtaaaccggg cattgccgca
     9541 ccgattatcg gtacatcgcg ggaagaacag cttgatgagc tgctgaacgc ggtggatatc
     9601 actttgaagc cagaacagat tgccgaactg gaaacgccgt ataaaccgca tgcggtcgta
     9661 ggatttaaat aagagtccag gcctgatgag acgtgacaag cgtcacatca ggcatcggtg
     9721 cacaactacg acagaatacc cagcggccag tgatgaccga taaagtacag gatgcctgcg
     9781 gaaatcaccc cggcgacaat atcgtcgatc atgatcccca tgccgccatg tacattgcga
     9841 tcaaaccagc ggatcggcca cggcttccac atatccagaa tacggaaaat cacaaacccg
     9901 gcggcaaccc actgccagtc attggtcggc agcgccatga gcgtgatcca cataccaata
     9961 aattcgtccc agacaatgct gccgtgatcg tgcacgccca tatctttcgc cgtttgatga
    10021 caaagataga cgccgataca gatccccaac atcaccacca gcgagtagag ctgccagggt
    10081 aaaaaggtca tcagatacca gaacggaatc gctgccagcg atcccatcgt gccaggaacg
    10141 attgggctta atccacttcc gaatccgaca gcaagcagat gccacggatt actcatcttc
    10201 aggcgacttt tcgcgacatc tttatggcgt ggcaaaatgg tcatatcctt tccagtcaaa
    10261 cgtgacaggt tcgccgtcac gaataaaaca aagcccttcg atatcggcgg tcatttgccc
    10321 gatacaggta aacggtacgc ccaggtgtcc gagagccaca tccagcgcgc cacggttcag
    10381 ttccggcaca gtgaaacaca actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc
    10441 ctgttccggt tcaacatggc gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat
    10501 acgtgcgccg cagtcgctgg ctttcacgat atgcccgaga tcggaaatca gaccgtcaga
    10561 gagatcgata gctgaatttg ccagatcgcg cagtgcctgt ccctgtaaaa tacgcggcga
    10621 tggacggaga tgacgtttga tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg
    10681 gttttgcaaa atcgccagcc cggcggcact atcgcccggt gtaccggtca catagatcca
    10741 gtcacccggt ttcgctccag agcgcgttaa ggctcgtccc atcggaacaa agccgtggat
    10801 acccaacgtc attgataatg gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta
    10861 ataattgaga agatcaaaca aactgtcgct gaaggactca agccacgctt cgtctacgtc
    10921 cggtaaggtt aatgccagcg tcagccaggc cggatcggcc cccatcgctg ccagatcgct
    10981 taggttcacc gccagtgctt tatacgccag atcagcagga tcgatatcat ggaggaagtg
    11041 gttgcccgcc accagcgtat cagtgctgat cgccagggtc tgtttctcgg ggatattgag
    11101 aagagcgcaa tcgtcgccga tgcccagttc gacatcaaga cgagaacttc ttacacggtc
    11161 aaaataacgg gcaatcaggg agaactcgcc acatgccata cgttatgcct cagcaggtaa
    11221 aaaagaaaag gccggcgatc gcggaacggt cttccgtgaa tctaccggcc tggatatcac
    11281 tttttgttag ggcgaatcac aggtgctgct ttatcgagta cgccgttgac gaacttatgg
    11341 ctgtcttctg cgccgaacga tttcgccagt tcgatcgctt cgttaatggc cactttgtat
    11401 ggcacatcgc tacgtttaga cagctcgtac agcgcaatgc gcagtactgc tttttctacc
    11461 tgtcccagtt cttccagcag gcgggacagg tatggcttca tcagtccgtc gaggtatgcg
    11521 gtgttagtcg ccaccccggc cagcagctca cggaagtaca ggacgtcaac gtcttttaca
    11581 tcctgttcag ccaggaactg gtattcaaca tcagcgatgt cgttctggga caactgccag
    11641 gagtagagcg cctggacggc acactcacga gcgcggcgac gagcagcagg tttcacggat
    11701 ttccccttac taatttcagg ccttgatggc tttcaataca ttaatcattt caagcgcggt
    11761 cagtgcagct tctgcacctt tgttgccagc tttggtgcca gcacgttcga tcgcttgttc
    11821 aatgctttca gtggtcagaa ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg
    11881 cgccaggccg ttgcttgcac caccagcgac atattcaaag tgggcagtgc caccacgaat
    11941 aaccgtaccc agcgcaatca ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc
    12001 cagcggcagc tcataggcac caggcaccca aacaacggta atgttttcat cttttacctg
    12061 accgatacgt ttcagtgcgt caattgcacc ttccagcagg ctgtcattga taaagttgtt
    12121 gaaacgcgcg atggtgatgg cgacgcgagc gtccggggta gcaacgttag cttcaataat
    12181 gttcattttc ttccttcggg ttcgagtatg gccccgcagg ggggcggatt ttagcataat
    12241 atttcgtgcg ctgcttccct ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa
    12301 catccgggcc tacatgacgt atctctttga atttaaattg gggggcgtcg gctaatttct
    12361 caagccctgg cagcgagcat aatccacggg cgtcgctgcc taatagttta ggtgcgatat
    12421 agacaatcag ctcatcgact aaacccacct gcagcaatgc gccagcgagc gttggccccg
    12481 cttccaccca gatgctgtta atttgctgtt tacccagttg catcatcagt acaaccagat
    12541 ccagatgacc tttatgctct ggaatcagca aggtacgcac cgtttccggc cactcacgag
    12601 aatcttcctg cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg cgatgttccg
    12661 gcgtcacgcg attttggcta tcaatcacaa tacgtaccgg ctgacggaga ttttgttgcg
    12721 ggtagagcac ctgagtttgt tcatccagtt cggaccaacg caccgttaag gcaggatcat
    12781 ccgccagcac cgtggcgctg ctggttaaaa tggcatgact ttgcgcgcgc agtcgttgta
    12841 cgtcgcgccg cgcctgagtt gaagtgatcc actggctttc gccgctcgcc atcgccgtgc
    12901 ggccatcaag cgatgcgcca agttttaact gaatataagg aaagccggtg cgcatccgct
    12961 tgagaaagcc tttattcaat tgctcggctt cactcatcat cagcccgtgg ctgacgtcaa
    13021 tgccagcctg ttgcagacgg taaagtccac gcccagcgac ctgcgggtta ggatcttgca
    13081 tcgctgcgac cacgcgcgct acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac
    13141 gaccatgatg gctacagggt tcgagtgtga cataggcggt cgcacctttg gctttttcac
    13201 ccgccatacg caacgcgtgt acttcggcat gtggttcacc cgctcgttgg tggtaacctt
    13261 caccgacaat ttcgccatct ttgacaatga cgcacccgac attcgggttg ggatgcgtgg
    13321 taaaacgtcc tcgttgcgcc agctttagcg cccgcgccat gtaatactcg tcctgcacgg
    13381 cttagtcctc caggcgcgcg atctcttcgc caaattcttt gatatcttcg aaactgcggt
    13441 agacagaggc aaaacggata taggcgactt tatcgagctt tttcaattgc tccatcacca
    13501 gattgccaat catcttgctc ggtacttcgc gctcaccggt ggcgcgcaac tgcgatttaa
    13561 tatgattgat tgccatttca acgtcatcgg aactcaccgg acgtttttcc agcgcccgca
    13621 gcattccgct gcgcaatttc tcttcattaa acggttcacg cacgtcgttg ctttttacaa
    13681 cacgcggcat aaccagctcc gccacttcaa aggtggtgaa acgttcatta cacaccagac
    13741 actgccgacg gcggcgtacg gatgaaccct cgcccacgag acgagagtca attaccttag
    13801 tgtccacggc gaaacagaat gggcaatgca tacggcgtcc tgaccaggtg gttaacggaa
    13861 atctatttta ccctgaactg acgatacaac aaaggcgcaa cgcttttgag ataaaggatc
    13921 gatgctttcg ctgcttttac cctctaccat taggctaatt gcgacgagaa agggacgacc
    13981 tatgaatact aacgtttttc gactgctcct gctgggaagc ctgttcagcc ttagtgcctg
    14041 tgtgcagcaa agcgaagtgc gacagatgaa acacagcgtc agcgcgctga accaggagat
    14101 gacgcaactc aatcaagaaa cagtcaaaat cactcagcaa aacaggctga atgcaaaatc
    14161 cagcagtggg gtttaccttc tgcctggagc gaaaacaccg gcaagactga aaagccagat
    14221 cggtacttta cgtatgtcgc tggtgaatat tacgcctgat acagatggca ccaccctgac
    14281 gctacgtatt cagggtgagt cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata
    14341 tgggcaaatt cagggaacaa tagacaactt tcaggaaatc aatgtgcaaa atcaattgat
    14401 taacgcccct gccagcgtcc tcgcccccag cgatgttgat attccgttac agttaaaggg
    14461 gatttctgtg gatcagttag gctttgtacg tatccacgac attcaacctg ttatgcagta
    14521 aacgtatttc ggggcgattt tgtgcgtccc gcaacatctt tccccgtcat tttgttactc
    14581 tgcttacatc acccagattg atagtaaaag tttgcaacaa gggtgaaagt cagtacaatc
    14641 cctgcccgaa tgtgtgtaaa cgtgaacgca atcgattacg taaatgatag aactgtgaaa
    14701 cgaaacatat ttttgtgagc aatgattttt ataataggct cctctgtata cgaaatattt
    14761 agaaacgcaa tttgcgcctt tttcactccc gcaagggatt ttcaaacagt ggcatacata
    14821 tgaaaaaaac attactggca gccggtgcgg tactggcgct ctcttcgtct tttactgtca
    14881 acgcagctga aaacgacaaa ccgcagtatc tttccgactg gtggcaccag agcgttaacg
    14941 ttgtcggaag ctatcacacc cgtttcggac cgcagatccg caatgatacc taccttgagt
    15001 acgaagcatt cgctaaaaaa gactggttcg acttctatgg ttatgcggat gcgccggtat
    15061 tcttcggcgg taactccgat gcaaaaggta tctggaacca cggttctccg ctgtttatgg
    15121 aaatcgaacc acgtttctcc atcgacaagc tgaccaatac tgaccttagc ttcggtccgt
    15181 tcaaagagtg gtacttcgcg aacaactaca tttacgacat gggtcgtaat aaagatggtc
    15241 gccagagcac ctggtacatg ggtctgggta ccgacatcga tactggtctg ccgatgagcc
    15301 tgtccatgaa cgtctatgcg aaataccagt ggcagaacta tggcgcagcg aacgaaaacg
    15361 agtgggacgg ttaccgtttc aaaattaaat actttgtgcc gattaccgat ctgtggggcg
    15421 gtcagctgag ctacatcggc ttcaccaact tcgactgggg ttccgatttg ggggatgaca
    15481 gcggtaacgc aatcaacggt attaagaccc gtactaataa ctctatcgct tccagccata
    15541 ttctggctct gaactacgat cactggcact actctgtcgt agctcgttac tggcacgacg
    15601 gtggtcagtg gaacgacgat gcagaactga acttcggcaa cggcaacttc aacgttcgct
    15661 ctaccggctg gggtggttac ctggttgtag gttacaactt ctgattatga aaatgccggg
    15721 atttattccc ggcatttcag attgttaaca acgtgcgctt tgttcatgcc ggatgcggcg
    15781 taaacgcttt gtccgaccca caaaagcacg taaattcaat atattgctga aataacgtag
    15841 tcctgataag cgtagcgcat caggcaattt tacgttttaa tcacttcttc ttgttcatca
    15901 tcgctttcag gtcagcgaat gggttgtact tcgcttcacc gacatctttc tgcgcatctt
    15961 cccctgcgat aacggtcgta ccatactgat ccgcttcggt atatttcgaa tgctcatgat
    16021 cgtggcaata gagacaaagc aattcccagt tactgccatc ttccgggtta ttggtatggt
    16081 cgtgatcaat gtggtgaaca gtaagttcac gcaggttgga atagacaaac tcgcgggaac
    16141 agcgaccgca gacccacgga tagattttta atgctttttc gcgatagccg ctttctaacc
    16201 gcgcatagtt ttttgggatg atagccatga aaagttacct ggatgaaatg tagggttacg
    16261 cgcagtttcc caagatgcgg ggattttcgc aatctccata catcaaaaaa aatcccgatc
    16321 ttctgaccgg gattttcaac atcaacggga acttgattaa ggcagaattg acggctgatc
    16381 cgccccttct ttttccactt tctgctgcaa catgtgttcg cgcttcatac ccagtttcag
    16441 agccaacgca gatgccacat agatggaaga tgcagtaccg atggaaacac cgataagcat
    16501 ggtcagcgag aagccttcca gtaccggacc accgaagagg tacagcatca ggataaccat
    16561 caaggtagta ccggatgtga tcaaggtacg gtgcagcgtc tgggtcaagg acacgttaaa
    16621 gatttcgtaa ggcgtaccgc gacggatctt gcggaagttt tcacgaatac ggtccgatac
    16681 cacgatactg tcgttaagcg agtaaccgat aaccgacatc aacgatgcca caatggtcag
    16741 gtcaatctcg atatggaata acgacaaaat acccagcgta atgataacgt cgtgcgccag
    16801 cgcaataacc acccctgccg ccagtcgcca ctcaaagcgg aaacctacgt acacgaggat
    16861 agacagcagc gctgccatca acgccatcgc accggtttgc gcaaggtctg cccccacgct
    16921 cggaccgacg aactcaatac gcttcactgc tgcattctga ttggtggatt cgttaatcac
    16981 cttcagaacc tggctgccca gcacctgacc gccggtttcg ccttcagcag gcggcatacg
    17041 gaccatgatg tcgtggctgc taccaaagtt ttgcagcatt ggctcttcaa aaccggcttt
    17101 ttgcaatgca tcacgcatta cgtcaatttc agccggtttt tcgagcgtaa tttcgataac
    17161 cgtaccaccg gtaaaatcca gcccccagtt aaagccgcgc acgcccataa taacgatagc
    17221 agcgattaac agcagaccag atatgccgaa agcccagtag tcccagcgca taaagtcata
    17281 gactttacgg ccgtggttta gttgttcaac agtatattcc tgtgccacat cgcactcctc
    17341 agattgacag cttcttgacg cgcttgccgc catatagcag gtttacgatg gcacgcgtac
    17401 cgacaatcgc ggtaaacatc gacgtcgcca caccgatacc ggtagtaatc gcgaaccctt
    17461 taattgcccc ggtacccact gcgtacagga tgatgacttt aatcagcgtg gtgatgttcg
    17521 catcgaagat agaactgaat gcgccacgat aaccttcatc aattgcctgt tgaacagtac
    17581 gtccgttgct caactcttct ttaatacgtt cgttgatcag tacgttcgca tcgaccgcca
    17641 ccgcaagggt taagacgata cccgcgatac ctggcatact cagcgttgcg cctggcaaca
    17701 gcgacataat gccgacgatt aagatcaagt tggcaatcag agcactggtc gcaatcagac
    17761 caaacttctt atagaagatg atcatgaaca gaatagaaac cagcagaccg gcaaggcaag
    17821 cctccagccc ctgttcaatg ttctgcatac ccagggttgg gccaatggta cgttcttcaa
    17881 caatctgaat cggcgcgatc aacgcaccgg cacgcagcag cagtgacagc tgacgggctt
    17941 cgttcgggtt gttgatgccg gtgatacgga agctgttacc cagacgagac tggatgttgg
    18001 cgatgttaat cacctcttcc tgtttcacca gaaccgcacg accatttgca tctttcttac
    18061 cgctgtcttt gtactccaca aacagggttg ccatcggttt gccgatgttg tccttagtga
    18121 agttagacat gatgttacca ccagcgctat cgagcgagat gttaacctgc ggttggttgt
    18181 attcgtcctg gctggaagtg gagtcggtga tatggtcacc ggtcagaatt acgcgtttgt
    18241 acagcacaac tggctgacct tcgcgggtct gtttcacttc agagtcgccc ggtacgcgac
    18301 cggatgccgc agcggcctgg tcaacgttgg tgtttaccag acggaattcc agcgttgccg
    18361 tcgcacccag aatctctttc gcacgcgcag tgtcctgaat acctggcagt tcaacaacga
    18421 tacggtcagc accctgacgc tgaaccaccg gctccgccac gccaagttgg tttacacggt
    18481 tacgcaggat attaatgttc tgctgcaccg catattcacg cgcttcactc agacgagcat
    18541 cgctcattac cgcacgcagc tggttgctgc cctggctgct aatcaccagg tccggatggc
    18601 gcttgctcag atacgcaatg gcttcatcac gagctttagc atcgcggaaa gtaatgctca
    18661 ggccgtagtt gttttcttta cgaacggtgg tatacgggat gcccttttca cgcagatcgc
    18721 tgcgcaggct atcgatattt tgttcctgga gtttgccaag cgcggtgtcc atatccactt
    18781 ccatcaggaa gtgaacgccg ccacgcaggt caaggccgag cttcatcggc tcagcgtgaa
    18841 tagctgccag ccagcgcggc gttgccgggg caaggttaag cgccacgacg tatttgtcac
    18901 ccataacgcc cattaatgct tcacgagcgc gcagctgggt gtcagtggag tcgaagcgcg
    18961 caagaatagc gccctcttcc agtgccacag acttagcagt tattttttct tcttgtaacg
    19021 ttttctggac ctggatcagc gtttgctcac tggcggcgac tccgcgcgca ccagtgatct
    19081 gaacagccgg atcctcacca aacaggttgg gaagcgcata cagcagaccg atgacaatca
    19141 ccacgatcag catgacgtac ttccacaaag gataacggtt taacacggca attcccttag
    19201 ggaaaaattt taattacagc gccttcatgg tgcctttcgg caggacggca gctacgaagt
    19261 cacgtttaat aactacttca gtggtgtcat tcagcgcgat agcaatgtag ccgttttccg
    19321 ctactttggt tacgcggcca accaggccac cgttcgtcag aacttcatca cctttggcaa
    19381 tggagtccat cagctttttg tgttctttgg tgcgcttctg ctgtggacgc aggatcatga
    19441 aatagaaaat cagaccgaat accaccagca tcaaaatcaa agacatcggg ctaccttgcg
    19501 ccggtgcacc cgttgccgct accgcatcag aaataaaaaa gctcattaaa tttccctcat
    19561 tattaatatt aatcaacgtt caaaggtggt acttctcgcc cctgacgctg gtaaaaatca
    19621 gttacgaagc tctctaattt accctcttca atagccttgc gtaaacccgc catcaaacgc
    19681 tggtagtaac gaaggttatg aatggtgttg agtcgcgcgc ctaatatttc gttgcaacgg
    19741 tcaagatgat gcaagtaagc gcgtgaataa ttgcgacagg tgtagcaatc acactcagga
    19801 tcgagtgggc cagtatcgct cttatacttc gcattgcgga ttttcaccac gccatcggtc
    19861 acgaacaaat gaccatttcg ggcgttgcgg gttggcatta cgcagtcaaa catatcgata
    19921 ccacgacgta cgccttcaac caggtcttct ggtttaccaa cgcccatcag gtaacgcggt
    19981 ttgtctgccg gaatttgcgg gcatacatgc tccagaatgc ggtgcatatc tgctttcggc
    20041 tcacccacag ccagaccgcc gacagcgtag ccatcaaaac cgatatctac cagaccttta
    20101 acagaaatat cacgtaaatc ttcgtaaacg ctgccctgaa tgataccaaa cagcgcattt
    20161 ttgtttccga gactgtcaaa acgctcacgg ctacgcttcg cccaacgcag agacatctcc
    20221 atggagcgtt ttgcgtaatc ccagtcagca ggatacggcg tacactcatc aaagatcatg
    20281 acgatatccg aaccaagatc gtactgaatc tccattgatt tttcaggatc gaggaaaatc
    20341 ggatcgccgt tgatcgggtt acggaagtgc acgccctgtt cggtgatttt acgaatatcg
    20401 ccaaggctga agacctggaa accgccggag tcggtgagga tcggcccctt ccactgcata
    20461 aaatcgtgca gatcgccgtg cagtttcatg atttcctggc ccgggcgcag ccacaggtgg
    20521 aaggtgttgc cgaggataat ttgcgcgcca gtggcttcaa cttcttccgg cgtcatccct
    20581 tttacggtgc cgtaggtgcc aacaggcata aaacaaggcg tttccactac gccacgatca
    20641 aagaccaggc ggccacggcg tgcgcgaccg tcggtggtgt ccagttcaaa tttcattttt
    20701 tctccactac gtcagaaaaa cagtccaacg ttttaaacca gcgccgcgga attactcccc
    20761 gacgcgctca ttaattgcct gcggattgta cgtgataaac atcgcatcac cgtaactaaa
    20821 aaagcgatat ttctcttcta ccgctgcttt ataggcgttc atggtgtgtt gataaccggc
    20881 aaaggccgaa accagcataa tcagcgtcga ctctggcaag tggaagttcg tcaccagcgc
    20941 atcgaccact ttgtactgga agcccggata gataaagatt tgggtatcgt cgaagaacgg
    21001 ttcaatgaga tcgtttttcg ctgcctgagc cgcgctttcc agcgaacgta ctgaagtggt
    21061 gccaaccgca atcacccggt taccgcgcgc tttcgccgcc agtaccgcgt ctaccacatc
    21121 ctgcggtact tcagcgtatt ccgagtgcat gatgtgatct tcaatggtgt cgacgcgcac
    21181 cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac gccatctcca cgcctttggc
    21241 gcgcaatttt tccagcaaag gctcgtcaaa atgcagacca gcggtcgggg ctgcaaccgc
    21301 gcccggtttt tcgctataaa cggtttgata aagttcgcgg tcagcgtctt cgtccggacg
    21361 gtcgatatac ggcggcagcg gcatatggcc gatactgttg aggatatcca gtaccgaacg
    21421 atcatcattg aattcgactt caaacagtgc gccgtggcgc gcggtcattg ttgcgttaat
    21481 actttcgtca tcgcccagca gcagttctgc gccaggtttt ggcgctttcg aggcgcgaat
    21541 atgcgcaaga atgcgtttgt cgtcgagcat ccgctcaacc agcacttcaa tcttgccgcc
    21601 gctggcttta cgcccaaaca ggcgcgccgg gatcacgcgg gtattattaa aaaccagaag
    21661 atcgccgggg ttgagcttat caagtaaatc ggtgaaagta ccgtgcgtca gcgcgcccgt
    21721 cggcccgtcc agcgacagta aacgacagct actgcgttca ggcatgggat agtgggcaat
    21781 cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc atgacactga ctcagactaa
    21841 aataagaggc gggtagtcta gtgccggggc gctctccctg caacctttac ccccttttgg
    21901 atagaatata atcgatgaat tttttagctc acctgcattt agcccatctc gcggaaagct
    21961 cgctttccgg caatttactg gctgatttcg tacgcggaaa tcctgaagaa agttttccgc
    22021 ccgacgtcgt ggctggcatt catatgcatc gacgtatcga cgtattaacc gacaatctgc
    22081 cggaagtccg cgaagcacgg gaatggtttc gtagtgaaac tcgccgcgtt gcgcctatta
    22141 cgctggatgt catgtgggat cactttcttt cccgccactg gtcgcagctg tcgccggact
    22201 ttccgctaca ggaatttgtc tgttatgccc gcgagcaagt gatgacgatt ttgccggact
    22261 caccgccacg ttttatcaat ctgaacaatt acttgtggtc agagcagtgg ctggtgcgct
    22321 atcgcgatat ggatttcatc cagaacgtgt taaacggcat ggcaagccgc cgcccacgtc
    22381 tggatgccct gcgtgactcc tggtacgatt tagacgctca ttatgacgcc ctcgaaaccc
    22441 gcttctggca gttttatccg cggatgatgg cgcaggcgtc acacaaggcg ttataaaacg
    22501 ttagttcatc cataccgtag ccgaaatggc aggcaaagcc agaatcccgt ttgtcagttg
    22561 cccatggcct tctttgcgtt gccattgcgc gacattaaga aacggtgacg cggggagcac
    22621 cacttcacag gcctcgccac ggttgattgc caccagtaca cgttgctgat tcagcacgcg
    22681 gacaaatacc actacgttat cttccgcata cagcacctga cagccgccac gacgcagcgc
    22741 ctgacttttc ttacgcagcg caatcattcg ctggtacagc gcgaataacg ccgtatcctg
    22801 cttttccacc tgccagggga acggtttacg gcaaaacgga tcgtttttgc catccagtcc
    22861 tacttcatca ccgtaataaa tgcacggtac accaggccag gtgaacagcc agaccaccgc
    22921 cagcggcagg cgcgcaatat cccgaccgag cagcgtttta aatcgcgcag tatcgtggct
    22981 gtcgagctga ttaaacatac gtaattgttg ttgatgagaa agccctgcgc ggtaattatc
    23041 catccaggcc atacaggttt gggcatcaat ttgctgcgga tcgtaagaga tatcggtatt
    23101 ggcaagaaat ccccacaacg ggaatgtgaa gccacgatag ttcatggcgg catcttccac
    23161 atcggcctgt aaccattgcc gtgcatcgcc aaaatgttcg ccgacaatat acgcttccgg
    23221 ctgggtttct ttcgccgctt cggtgatccc agcaacgtgc tgcatattat tgcgcgcccc
    23281 acccgcctcc cccagcatat gcaccacatc cagccgccag ccgtccatac tccacggcgc
    23341 tttcagccaa tggcggacaa tactgtcttc cccgcgataa atttcattca ccagactttc
    23401 cgactgataa tccagcttcg gcaagctggc atagccaagc cagtcgagcg ccgtgccatc
    23461 atcactaaac gagtaccagt cgcgccaggg cgattcaggg ttgtgacaag ctccccccgt
    23521 gccacgattg tgcctgtcaa accaggcatg ggaatcgcca ctgtggttaa acacgccatc
    23581 cagcaccagc cgcattccca gctgctgcgt attgtggcgt aagcgcagca acgccccatc
    23641 accgccaaac tgcggatcga catggcgata atcctcggta tcgtatttat gtacgctggg
    23701 agctttaaac accggattga gatacagcgc cgtcacgcca agctttttca gatacggcag
    23761 tttttcgctt atcccgtcca gatcgccgcc atagaacgtt gatccgcccg cctgcgccgt
    23821 gaccggttca tcccagtcac gcaagatgat ctcttgtccg gctgcatgat ggtaatagac
    23881 atgatcctgt tcagcttcac gaggaagact acgcgcaaaa cgatcaggga agatctgata
    23941 aaaaatctga tccgcagccc attgtgggcc gatatccggt acatcgacgg caaactgctc
    24001 cagtcgtgcc ggcggcattc ggctgaagcc ctgcggtgta aaccaacgct ggcgatcgtg
    24061 ccacagcagt ttgaaactgt aacgccgccg gggttgtccg ctggagagat caatcgccgc
    24121 acgccatgcg gtgacgccag gctgctgctg actgcgctgc ttatgcatcg atactgacat
    24181 ttcttcgtta tcgtgttctg tacgcagcat aatgcgctgc ggtgggtctt cgcccgtcag
    24241 ccacagtgta atgagcagtt gatctttgct ttgtttaaca aatgggggca ccggcaggtg
    24301 ccatgcattt aacatcataa ttcccctgtg atgaaaatct gatcaccttg ccatagccta
    24361 atgcaggatc actcatcatc tgaaggatat ttgggggagg aggacggggg cggataactt
    24421 tcggaatagc cggatcagga tttcctcctg aaccggctta cagaacgcaa ggattactgg
    24481 ttttcagcca gctgacgatc gtggcggcgt ttaaacatcc agccaatcaa cagcacaaca
    24541 atccacgcga aaccgacata cagcgagata cgcgtatccg ggtgataacc aatcaacccg
    24601 ataataaaga gcaggaaaat caaaccgccg atggtcgttg ctaccccacc cggcacttta
    24661 aatttcagcg ccttaacttc ttctggcggc aaacggcgac ggaaggcaat ttgcgacagc
    24721 aggatcataa tccacaccca caccgtggcg aaggttgcca gcgaagcgat caccaggaag
    24781 acgttttccg gcatgatgta gttcagatac accgcaaaca gcagcgcggt agtcatcacc
    24841 agcaccgtaa cccacggaat accgcgacgc gacgttttgc tgaaaatttt cggcgcgctg
    24901 ccctgctctg ccataccgtg gagcatacgg cctacgccaa atacgtcact gttaattgcc
    24961 gacagcgaag cagtcagcac aacaaagtta agaatgctgg cggcaaaggt aatgcccata
    25021 tgctggaacg tcagcacgaa cgggctaccg gcagtgccaa cctgattcca cgggtagata
    25081 gacataatga cgaacagcgt accgacgtag aacaccagaa tacgcatcgg cacggagtta
    25141 atcgcacgcg gtatcgattt ctcaggatct ttcgcttcac cggcggtaat cccgataatt
    25201 tcgatcccac cgtaagcaaa catcaccatt tgcaacgaca tcaccatccc aagccagccg
    25261 ttactgaaga agccgccgtt gctccacagg ttatgaatac cggtcggttg cccgccgttg
    25321 ccaatccccc agatgatgat gccgaaaccg gcgacaatca tgatgatgat ggtggcgact
    25381 ttaaagaacg agaaccagaa ttccagctca ccgaagacct tcacgctcat caggtttacg
    25441 gcgcaaatga tcagcaccac gctcagtacc caaatccagt gcggcaccgt cgggaaccag
    25501 acacccatat agataccaaa agcggtcaca tcggcgatgg cgacaataag gatttcaaag
    25561 caataggtcc agccggtaat gtaacctgcc agcgggccga ggttttcctg cgcataacgc
    25621 gagaaagagc tggctgccgg gttatgtacc gacatttccc ccagcgcacg cataatgata
    25681 tacgccgcga taccaccgat aatataggcc aacaacacgc tcggaccggc cattttgatg
    25741 gcgtctgccg aaccgtaaaa cagcccggtg ccaattgctg aacccagtgc cataaagcgt
    25801 atgtggcggg tacttagccc acgctttagc ttgttcttac tttccatcaa ttcctaaccc
    25861 atcaacacaa taaaaaacca cggggcatcg cagccccgtg gttaaaacaa atgttcagtg
    25921 atttagtgag cgctggaggt cacctgacga cctgccgcgc gatcccagat aatggccaga
    25981 accaccatca ccactgttgg cattaaccac gccagacctt gttcggccag cggtaaacgc
    26041 tgcgcccagg acggtaagat atcgctgaat gcagatgctt tgatcccgtc gagaatacca
    26101 aaaagcaggc tgataaacat cggcggagca atcacgcggg acgaattatg ccaccatgag
    26161 cgtgtaaaac ttaatacaac cagtgcgata cacggcggat aaatagcggt cagcaccggt
    26221 acggagatct ggatcagctg gcttaagccg aggttagaaa ccaccatcga gaagccgccg
    26281 aggataaaca ccagcgtacg ataagagagc ggtacgtact gggcaaagaa ttctgcacaa
    26341 gcacaggtca ggccaactgc cgttaccagg caggcgatga agattaacgc cgccagcagg
    26401 aagctaccgc cgccgccaaa ggtgtgctga acgtaagcat gcagaatagc agcgccgttt
    26461 gcagactgat cgaccagcga cgcgctgtct gaacccagac ggaacagcgc caggtacagc
    26521 agagtcagac caacacccgc catcaggcca gcccagacgg tataacgggt cagcagacgc
    26581 gcttcggtaa cgccacgaga acgcgccgcg ttaacaataa cgataccaaa caccattgcg
    26641 cccagcgtat ccatggtcag atagccgtta acgaagccgt tagaaaacgc agcgttttga
    26701 taagcctcag tcgccgtgct gatagaaccc gccggccaga caatcgcggc aacagacagg
    26761 atgaccagcg cgataatttt cagcggcgca aggaagttgc ccacggtatc cagcagcttg
    26821 cccggataga gcgaaaccag aataacgata gcgaaataga ccaggctgta gataaacagc
    26881 ggcagcgcgg aatcaccagt cagcggcgca atccccactt caaaggaaac ggtagctgta
    26941 cgcggcgtag cgaaaagcgg ccccaccgcc aggtaacaaa ccgttgccag cagtacgcca
    27001 gcgactttac cgattggcgt gctgaggctg tcaacaccgc cgccaacttt tgccagcgcc
    27061 actaccgtta gtaccggcag accaacggca gtaatgagga agccgaatgc cgcagtccag
    27121 acgtgttcgc ctgcctgcaa gccgaccatt ggagggaaaa taatgttacc tgcgccgacg
    27181 aacaacgcaa atgtcataaa gcccagagcg atgatatcgc gcgatcttaa ttgatgggtc
    27241 ataaatcata ctgcctgtgg atgtggtgtt gtaacgttaa atttttgtgc caatccctgg
    27301 ggatgataca gcggtatatt taaacaattt tagcgggaaa tactcccaac caggcgcggc
    27361 gaagaatagt ttttcgattt accgcgttaa tgcagtcagt atgacagcac ctgaagcgca
    27421 atttaaacgc ttataacgtt ttaaagcaag gagggatcta aaaaccagag gtatatagcg
    27481 ataagaaatc accaaccagt ataaaacact atttccgtga aaaagacatg gctaatcatg
    27541 cgaacaaaaa atcaccaacc gttgtaaaat ctaacgcccg tattttgact gcaaagaaaa
    27601 taagccagca tcggctggct tatggcaata aaagatgaca aaggcggatt aatcgctgtt
    27661 tttggcaatt aaacgttccg ggataacaaa actgaaacgt gttccttttc ctactgtact
    27721 ctcaatattc aggcgacttt cgtgatgatt caccgcatgt ttcacgatcg ctaaccctaa
    27781 tccgctaccg ccggtttgcc gggaacgcgc tttatcaacg cgataaaaac gctcggtcag
    27841 gcgcggaata tgctccggtg caatgcccgg tccgttatct tcaacgctaa attcggcacc
    27901 gtgcggcact cgctgccagc gtacggtgat atgcgtgcct tctggagtat gattcacggc
    27961 gttatagacc aggttcgaaa tcgcactgcg tagctgatct tcgttgccag acactttgag
    28021 gccgttatct atctcaaagg taaatgtctg ttttttctga ctcagagtct gagcctcacg
    28081 ctcaacaacg cgcagcatca tcggcacatc aaccttttca ttgagcaaat gcgtcggtgc
    28141 ggcttctatt ttcgacagcg tcagcaactg cttcaccagc ccttccatcc gctgggtctg
    28201 ctcgcgcatg gtgtgcaacg ctttttcgcg taccgcgcct tccagcggct gctcatccat
    28261 catctccagg taaccctgta acacggtcaa tggcgtacgt aactcatggc tcacgttggc
    28321 aaaaaagtta cgccgcgccc cttccagttg atgcatttgc gtgacatcac gcgccaccat
    28381 cagcaactgt ttgtgggtat aaggcatgac gcgaatttcc agatgccgcc cggtgttgag
    28441 caccagattg agcgggcgag aaaaatcacg cgttttcaga tattgcgtaa actccgggta
    28501 acgcagcagg ttaaggatgt tctgcccgtt atcttccggc cagcgcaaac caagaatttg
    28561 ttgcgccaga ccgttacacc agaaaatacc gccctcttca gtggtcagca ccactgcgtc
    28621 gggcagcgac tccgcgccgc tacgaaagcg tttaatcaga ttgcccagtt cacggcggcg
    28681 ttttttattt cgcagctgca tctggtgtaa gccgtatagt agcggttccc agctaccacg
    28741 ccccggtggc ggggtcatac tacgatccac ccacagccac catgaaaggc gcaataaatt
    28801 ccagaaatgc cagataagca gtcccgttac cgatgccagc aaaaaccagg gcaggtaacc
    28861 aaaaaatgca cccaggatga aagccgggaa gcagcaaagt agcagctcca gcaccagcct
    28921 tttccacgac agccgttcca gcacgtaaga tactccagtt aagaaatcat aagccctgct
    28981 ctgcgtccga tgagcaaggc gttaaaagcg ggttgaaaaa cgatatcctg taccgcgcac
    29041 ggtctgcacc atgcggtcat gcccgcctgg ctccagtgct ttacgtaggc gacgaatgtg
    29101 gacatcgacc gtacgatctt ccacatacac gttagttccc cagacgtggt ttaacagctg
    29161 ctcgcggctg tacacgcgct caggatgcgt cataaaaaag tgcagcagtt taaattctgt
    29221 cggccccatc tccagcggct cttcgcccgc catcactcgg tgagatgtcg ggtcgagact
    29281 taatccctgc atctcaatca cctcttccac cgccattggc gaaatacggc gcattaccgc
    29341 tttgattcgc gccaccagct ccttcggcga aaacggcttg gtgatatagt catccgcgcc
    29401 ggtttcaagg ccgcgcacgc gatcttcttc ttcccctctg gcggtcaaca tcaccactgg
    29461 aatatcccgg gtcatcgact cgcgcttgag gtgtttgatg aactggatac cggagccgcc
    29521 aggtaacatc cagtcaagga gaattaaatc cggccagggt tcattcagtt gattcacagc
    29581 actgtcataa tcttccgctt cgaccggctg aaagccattt tgttcgagca cgaagcagac
    29641 catttcgcga attggagctt catcttctac gaccagaata cgtctcgcca tgatttgccc
    29701 tgttgtaata aataggttgc gatcattaat gcgacgtcat tatgcgtcag atttatgaca
    29761 gatttatgaa aagctcgtcg cacatatctt caggttattg atttccgtgg cgcagaaaaa
    29821 agcaaatggc acatctgttt gggtataatc gcgcccatgc tttttcgcca gggaatcgtt
    29881 atgcgcatcc ttcacacctc agactggcat ctcggccaga acttctacag taaaagccgc
    29941 gaagctgaac atcaggcttt tcttgactgg ctgctggaaa cagcacaaac ccatcaggtg
    30001 gatgcgatta ttgttgctgg tgatgttttc gataccggct cgccgcccag ttacgcccgc
    30061 acgttataca accgttttgt tgtcaattta cagcaaactg gctgtcatct ggtggtactg
    30121 gcaggaaacc atgactcggt cgccacgctg aatgaatcgc gcgatatcat ggcgttcctc
    30181 aatacgaccg tggtcgccag cgccggacat gcgccgcaaa tcttgcctcg tcgcgacggg
    30241 acgccaggcg cagtgctgtg ccccattccg tttttacgtc cgcgtgacat tattaccagc
    30301 caggcggggc ttaacggtat tgaaaaacag cagcatttac tggcagcgat taccgattat
    30361 taccaacaac actatgccga tgcctgcaaa ctgcgcggcg atcagcctct gcccatcatc
    30421 gccacgggac acttaacgac cgtgggtgcc agtaaaagtg acgccgtgcg tgacatttat
    30481 attggcacgc tggacgcgtt tccagcacaa aactttccac cagccgacta catcgcgctc
    30541 gggcatattc accgcgcaca gattattggc ggcatggaac atgttcgcta ttgcggctct
    30601 cccattccac tgagttttga tgaatgcgga aagagtaaat atgtccatct ggtgacattt
    30661 tcaaacggca aattagagag cgtggaaaac ctgaacgtac cggtaacgca acccatggcg
    30721 gtgctgaaag gcgatctggc gtcgattacc gcacagctgg aacagtggcg cgatgtatca
    30781 caggagccgc ctgtctggct ggatatcgaa atcactgctg atgagtatct gcatgatatt
    30841 cagcgcaaaa tccaggcatt aaccgaatca ttgcctgttg aagtattgct ggtacgccgg
    30901 agtcgtgaac agcgcgagcg tgtgttagcc agccaacagc gtgaaaccct cagcgaactc
    30961 agcgtcgaag aggtgttcaa tcgccgtctg gcactggaag aactggatga atcgcagcag
    31021 caacgtctgc agcatctttt caccacgacg ttgcataccc tcgccggaga acacgaagca
    31081 tgaaaattct cagcctgcgc ctgaaaaacc tgaactcatt aaaaggcgaa tggaagattg
    31141 atttcacccg cgagccgttc gccagcaacg ggctgtttgc cattaccggc ccaaccggtg
    31201 cggggaaaac caccctgctg gacgccattt gtctggcgct gtatcacgaa actccgcgtc
    31261 tctctaacgt ttcacaatcg caaaatgatc tcatgacccg cgataccgct gaatgtctgg
    31321 cggaggtgga gtttgaagtg aaaggtgaag cgtaccgtgc attctggagc cagaatcggg
    31381 cgcgtaacca acccgacggt aatttgcagg tgccacgcgt tgagctggcg cgctgcgccg
    31441 acggcaaaat tctcgccgac aaagtgaaag ataagctaga actgacagca acgttaaccg
    31501 ggctggatta cgggcgcttc acccgttcga tgctgctttc gcaggggcaa tttgctgcct
    31561 tcctgaatgc caaacccaaa gaacgcgcgg aattgctcga ggagttaacc ggcactgaaa
    31621 tctacgggca aatctcggcg atggtttttg agcagcacaa atcggcccgc actgagctgg
    31681 agaagctgca agcgcaggcc agcggcgtcg cgttgctcac gccggaacaa gtgcaatcgc
    31741 tgacagcgag tttgcaggta cttactgacg aagaaaaaca gttacttacc gcgcagcagc
    31801 aagaacaaca atcactaaac tggttaacgc gtctggacga attgcagcaa gaagccagcc
    31861 gccgtcagca ggccttgcaa caggcgttag ccgaagaaga aaaagcgcaa cctcaactgg
    31921 cggcgctcag tctggcacaa ccggcacgaa atcttcgtcc gcactgggaa cgcatcgcag
    31981 aacacagcgc ggcgctggcg catactcgcc agcagattga agaagtaaat actcgcttac
    32041 agagcacaat ggcgcttcgc gcgagcattc gccaccacgc ggcgaagcag tcagcagaat
    32101 tacagcagca gcaacaaagc ctgaatacct ggttacagga acacgaccgc ttccgtcagt
    32161 ggaacaacga actggcgggt tggcgtgcgc agttctccca acaaaccagc gatcgcgagc
    32221 atctgcggca atggcagcaa cagttaaccc atgctgagca aaaacttaat gcgcttgcgg
    32281 cgatcacgtt gacgttaacc gccgatgaag ttgctaccgc cctggcgcaa catgctgagc
    32341 aacgcccact gcgtcagcac ctggtcgcgc tacatggaca gattgttccc caacaaaaac
    32401 gtctggcgca gttacaggtc gctatccaga atgtcacgca agaacagacg caacgtaacg
    32461 ccgcacttaa cgaaatgcgc cagcgttata aagaaaagac gcagcaactt gccgatgtga
    32521 aaaccatttg cgagcaggaa gcgcgcatca aaacgctgga agctcaacgt gcacagttac
    32581 aggcgggtca gccttgtcca ctttgtggtt ccaccagcca cccggcggtc gaggcgtatc
    32641 aggcgctgga gcctggtgtt aatcagtctc gattactggc gctggaaaac gaagttaaaa
    32701 agctcggcga agaaggtgcg gcgctgcgcg ggcaactgga cgccttaaca aagcagcttc
    32761 agcgcgatga aaacgaagcg caaagcctcc gacaagatga gcaagcactt actcaacaat
    32821 ggcaagccgt cacggccagc ctcaatatca ccttgcagcc acaggacgat attcaaccgt
    32881 ggctggatgc acaagatgag cacgaacgcc agctgcggtt actcagccaa cgacatgaat
    32941 tacaagggca gattgccgcg cataatcagc aaattatcca gtatcaacag caaattgaac
    33001 aacgccagca gcagctctta acggcattgg cgggttatgc actgacattg ccacaggaag
    33061 atgaagaaga gagctggttg gcgacacgtc agcaagaagc gcagagctgg cagcaacgcc
    33121 agaacgaatt aaccgcgctg caaaaccgta ttcagcagct gacgccgatt ctggaaacgt
    33181 tgccgcaaag tgatgatctc ccgcacagcg aagaaactgt ggcgctggat aactggcggc
    33241 aggttcatga acaatgtctc gcattacaca gccagcagca gacgttacag caacaggatg
    33301 ttctggcggc gcaaagtctg caaaaagccc aggcgcagtt tgacaccgcg ctacaggcca
    33361 gcgtctttga cgatcagcag gcgttccttg cggcgctaat ggatgaacaa acactaacgc
    33421 agctggaaca gctcaagcag aatctggaaa accagcgccg tcaggcgcaa acgctggtca
    33481 ctcagacagc agaaacgctg gcacagcatc aacaacaccg acctgacggg ttggctctca
    33541 ctgtgacggt ggagcagatt cagcaagagt tagcgcaaac tcaccaaaag ttgcgtgaaa
    33601 acaccacgag tcaaggcgag attcgccagc agctgaagca ggatgcagat aaccgtcagc
    33661 aacaacaaac cttaatgcag caaattgctc aaatgacgca gcaggttgag gactggggat
    33721 atctgaattc gctaataggt tccaaagagg gcgataaatt ccgcaagttt gcccaggggc
    33781 tgacgctgga taatttagtc catctcgcta atcagcaact tacccggctg cacgggcgct
    33841 atctgttaca gcgcaaagcc agcgaggcgc tggaagtcga ggttgttgat acctggcagg
    33901 cagatgcggt acgcgatacc cgtacccttt ccggcggcga aagtttcctc gttagtctgg
    33961 cgctggcgct ggcgctttcg gatctggtca gccataaaac acgtattgac tcgctgttcc
    34021 ttgatgaagg ttttggcacg ctggatagcg aaacgctgga taccgccctt gatgcgctgg
    34081 atgccctgaa cgccagtggc aaaaccatcg gtgtgattag ccacgtagaa gcgatgaaag
    34141 agcgtattcc ggtgcagatc aaagtgaaaa agatcaacgg cctgggctac agcaaactgg
    34201 aaagtacgtt tgcagtgaaa taactattca gcaggataat gaatacagag gggcgaatta
    34261 tctcttggcc ttgctggtcg ttatcctgca agctatcact ttattggcta cggtgattgg
    34321 tagccgttct ggtggttgtg atggtggtat gaaaaaagtc attttatctt tggctctggg
    34381 cacgtttggt ttggggatgg ccgaatttgg cattatgggc gtgctcacgg agctggcgca
    34441 taacgtagga atttcgattc ctgctgccgg gcatatgatc tcgtattatg cactgggggt
    34501 ggtggtcggt gcgccaatca tcgcactctt ttccagccgc tactcactca aacatatctt
    34561 gttgtttctg gtggcgttgt gcgtcattgg caacgccatg ttcacgctct cttcgtctta
    34621 cctgatgctt gccattggtc gcctggtttc cggctttccg catggcgcat tttttggcgt
    34681 cggcgcgatc gtgttatcaa aaattatcaa acccggaaaa gtcaccgccg ccgtggcggg
    34741 gatggtttcc gggatgacag tcgccaattt gctgggcatt ccgctgggaa cgtatttaag
    34801 tcaggaattt agctggcgtt acaccttttt attgatcgct gtttttaata ttgcggtgat
    34861 ggcatcggtc tatttttggg tgccggatat tcgcgacgag gcgaaaggaa agctgcgcga
    34921 acaatttcac tttttacgca gcccggcccc gtggttaatt ttcgccgcca cgatgtttgg
    34981 caacgcaggt gtgtttgcct ggttcagcta cgtaaagcca tacatgatgt ttatttccgg
    35041 tttttcggaa acggcgatga cctttattat gatgttagtt gggctaggga tggtgctggg
    35101 gaatgtgcta agtggcagga tttcaggacg ttattcacca ctgcgcattg cagcagtaac
    35161 tgactttatc attgtactgg cactgctgat gctttttttc tgcggcggca tgaaaacaac
    35221 gtcgcttatt tttgctttta tttgttgcgc gggattattt gccctttcag cacctctgca
    35281 aatattgtta ctacaaaacg ccaaaggcgg agagttatta ggtgccgcag gtgggcaaat
    35341 agcgtttaac ctcggtagcg ccgtcggcgc atattgcgga ggtatgatgc tgacgctggg
    35401 gctggcatat aattacgtgg cgctgcctgc cgccctgctt tcgtttgctg cgatgtcatc
    35461 gttgctgctg tatggtcgct ataagcgcca gcaagcggcg gatagtccgg tgctggcgaa
    35521 accactgggg tagattactg tcacggtgcg cgttgcttat tgccggatgc ggcgtgaacg
    35581 ccttatccgc cctacgcggt tccggcacat tttgtaggcc tgataagacg cggcaagcgt
    35641 cgcatcaagc atgatgcgcc aattgcctac gttttttact cttgtggcca taaccacgca
    35701 gcgccgcgta cgccgctgga atcaccgtgc ttcgccttac gcaccggcgt ttcacattcg
    35761 ccgccgaaga caaattgttt aatcaactgc ccaaccgttt gatataaacg gtctacattg
    35821 ctcatcccgc cccccaggac aatcacatcc ggatcgagaa tattcacgac atgtgccagc
    35881 gattttgcca gccgcagctc gtagcgacgc aatgccagtt ccgctaccgg atcgctttct
    35941 tcaaccaggc ggataatttc actgcctttc agcgcatgtc cgctcaaacg acgataatct
    36001 gtggcaaatc ccgtgcccga aataaaggtt tcaatacaac cttgtttacc gcaataacaa
    36061 gggacttcct cgcgataacg cagttcgtct tcgtccatcc acggtagcgg attgtgtccc
    36121 cactcgcctg ccgtgccatt gccgccgata tgcgcccgcc cattgaatgc cacgcccgcg
    36181 ccgcatcccg tgccgataat cacggcaaat accgtctgtg ctcccgctgc cgcgccatct
    36241 actgcttctg aaacggccag acagttggcg tcatttgcca gccgcacttc ccgctgcaac
    36301 ctcgcgctta agtctttatc gaatggctga ccgttgagcc aggttgaatt ggcattctta
    36361 accacaccgg tgtaaggcga aattgagcca ggaatgccca tacctaccgt tccgcgctgc
    36421 cctgtcgcct gctccgccat atcaaccaac gtggcgatcg tttcaatagt ctgccggtaa
    36481 tcatcacgcg gcgtgggcag acgatggcgg tacaactgct cccctgcatc gcccagtgca
    36541 atcacttcag ttttggtgcc gcctaaatcg atacctatac gcacggtact ctccttattt
    36601 ttttcaatat caatagcgta gagacggaca accggattgg caatgcaagg ccgccgacaa
    36661 ttcgttatca tgcccgctaa atttaacgac aaggccgtgg aaattatcat gctgtggttc
    36721 aaaaatttaa tggtttaccg tcttagccgc gagatttcgc tgcgtgcaga agagatggaa
    36781 aaacagctag cctcgatggc atttacccca tgcggcagcc aggacatggc gaagatgggc
    36841 tgggttcctc cgatgggatc gcacagcgat gcgttaacgc acgttgccaa tggtcaaatt
    36901 gttatctgcg cgcgcaaaga agaaaaaatc ctcccgtctc cggtgattaa gcaggcgctg
    36961 gaagcgaaaa tcgccaaact ggaagcggaa caggcgcgta agctgaagaa aaccgaaaaa
    37021 gattcgctga aagacgaagt gctgcactct ctgttgccgc gtgctttcag ccgttttagc
    37081 cagacaatga tgtggatcga cacggttaac ggtttgatta tggtggactg cgccagtgcc
    37141 aaaaaagcgg aagatacgct ggcactgctg cgtaaaagcc tggggtcgtt accggttgta
    37201 ccattgagca tggaaaaccc gattgaactg acgttgaccg aatgggttcg ctccggtagt
    37261 gcggcacagg gcttccagct gcttgatgaa gccgagctga aatcgttgct ggaagatggc
    37321 ggcgtgatcc gcgcgaagaa acaagatctg accagcgaag agatcaccaa tcacattgaa
    37381 gccggaaaag tggtgactaa actggcgctc gactggcagc agcgcattca gtttgtgatg
    37441 tgcgacgatg gttcgctcaa gcgtctgaag ttctgcgacg agctgcgcga tcaaaacgaa
    37501 gatatcgacc gtgaagattt cgcccagcgt tttgatgccg atttcatcct gatgactggt
    37561 gaactggcag cgttaattca aaacctgatt gaaggattag gtggcgaagc acaacgttaa
    37621 ttgctgattt tcctttaatg ccggatgcga cgcctgccgc gtcttatccg gcgtacgaag
    37681 ccacaccagg catataatta ttcgctacgg cgggcaataa tttttagcgc agcaatatta
    37741 tgcgttttat gctgtaactt gctccatgga cgttgtgtca ttgtttttcc tcaagccagt
    37801 gacgcgcttc actgcgaatt ttccgcattg ccccggtttt cactaaatcc cgtaacagcg
    37861 aaagatcggg ttgttcgaca aataagagta atgatggatc ctcttttaac cacgtcgttt
    37921 tcacatcggc agcgagctgt ggattattca tagctagcga ccgatgccga gactcggtca
    37981 atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa tgccgtttgg cgcaaatgat
    38041 cattgcgact ttgcagcaat gcacgccacc attcatcatc tgatttatct tttatcgtag
    38101 ccagttccgc aacaatatct ggagatcttc actgatattt atatcaacct tgatgaatta
    38161 tgaatatacc ggaaattgaa aaaatcgcag caaaggaaat attattatgg gttaaagaga
    38221 ggatatttca gaaaaatgcc ctcccgttca aggggaaggc gaggaattac agatagcgac
    38281 acagataaga ggtgggttcg gcaacttgca gatgaaactc actgtgaccg ggaacattaa
    38341 acaccgaacc ggcttcatac acctgccagt cggtcgcgtc aggcagtaac acattcagcg
    38401 cgccactgat taccgtcatt tcttccggct cagcggtgct gaaggtgtat tcgccttcaa
    38461 ccataacacc cacgctggcg cgaccagtgc tgctgctgga aaagccgatt gatttcactt
    38521 tgccggaaaa gtactcatta ctttgaagca taaactggcc ctgtttggta gttgaacatc
    38581 tacttcacta taggggccag aggcgctggc tgtcacgcaa aattacacca gtaattccgc
    38641 agccagccgt gcaatcaata cgttagacag caagacagga acatcgagct gtttttgcag
    38701 taaatcgcga tgacgttggt gaaatcctaa acaatccagc atgatgacat cagccccttt
    38761 tgccagtaat tctttcccgg catcaatgat tttttgttct gaatcatgaa tggggttacc
    38821 caatgaaaat accggcgatt tctgcaaaat ttgccatttt tgcgcctgaa cgggcagcat
    38881 ctcctcaacc ggaacgataa cccccacctg atgatcttca acaatagagg aaaccagtgg
    38941 aggcaatatt cgcgacggct caagaaagat cgtattacgc gcagtcatac tactaatgtt
    39001 tgctgtactc attaataaaa tgacgtcata accctgatta tcgagcactt caaccacacc
    39061 ttgcaggtct cgctccactt tacgacgcga aacatgggcc agctggatgt catttaataa
    39121 tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc gccatcactt cttcacgact
    39181 taacttcccc agcaggctat gatgggaaat attatcttcg tcaatgtatt ccgtcaggag
    39241 cggcaaaacc tcctgcatag gtacaatgcc gatggtcagg atcgccaacg acgcactcat
    39301 tgttcatcca ccttactttt ctttcgtcgt taattaccgg gcaagtgtga agcaccactg
    39361 ctgacactta cttgtgctac aaatgacaaa aagcgtagca gcagacgtgc acggcataat
    39421 gtaaagattc caaatgattc cagtaatgga ttgttattgt ttaatattct aattattaga
    39481 aaaacatgaa ttatgaaaaa tgtgacgcag atcccttgta gtggcactca gaatcccttc
    39541 cgagcagtct gatgcggtca ctctttgtca gggtcctcat agcgtttttt cgcatccatc
    39601 gcttcctgag ccgtcggatg ttcactgatc aacgagtctg gtttaggatg atcggctctg
    39661 agttggtacc aggttaccgt ctgtcctggc tttccttttt caatcgtcac tatatatgct
    39721 tcacgaggat aaggcggttt cgttggcatc gttcttcctt atttcacggg atgaacgtta
    39781 agtataggcg ctctaaaatc aacaattgat cgtctgtgcc agggcgctac gaatttcaga
    39841 aatcacctgg ctgggttcgt ttgttgcgtc gatgataata tgcgcaactt cgcgatatag
    39901 cgcatcgcgt tcttccagca cttcctgaac ttcttcgctc agcggttttc ccgttaaggt
    39961 tggccgtaaa tcttcttccg gtgcagcttg cagtcggtta accaggactg atactggcgc
    40021 acacaaataa accacgatcc cgttattttg cataaagtga cgattaaatt ccgtcagaat
    40081 aatgccgccg cctgtagcga taacggtgga tggcgcagtt accgcttcca gcgccgccgt
    40141 ttctctggcg cgaaatcccg cccactcttc cctttcgacg atctccgcga ccgtcatatt
    40201 gagctgtgat tgcaaccact gatcggtatc gacaaaccga cggttaagcg aatcggcaag
    40261 ggccattccg accgttgttt taccacagcc ccgaggcccg atcagaaaaa gaggttgtgt
    40321 catcgtgggt tttccccaat aggtcgcaat gcggcgaaag ccggtgtcat gagaatagcg
    40381 atcataccat caaactagta caatttcgat tgtaaagaaa aaattccact taaagtgaaa
    40441 atctcaatac atcccttact ataccaataa atattcaaga atgaagtgta aacaataaat
    40501 tacatttagc cacgactacg ttgcacttcc agccaccact tctccagctc cgcggcaaag
    40561 gcctggcggt cacgttgtga aaggctatct ggtccgccgg tctggatccc actggcacgt
    40621 aaggtatcca taaaatcgcg catcgtcagg cgctcacgaa tggtcgctgg cgtgtaacgt
    40681 tcgccgcgcg gattaagcgc cgctgcgcct ttctcgatgg cttcagcagc caaaggtata
    40741 tctgcggtga tcaccaaatc gcccgcttca cactgccgga caatttcgtt atcggcaacg
    40801 tcgaaacctg ccgcgacgcg cagcgtacga ataaatcgcg atggcggcac gcgtaaactc
    40861 tggtttgcta ccagtaccag cggcatctgc atacgttccg ccgcgcgata caaaatctct
    40921 ttaattacac tgggacacgc gtcggcatcc acccaaattg tcataaagtc atcctttgtt
    40981 gggtaatcct ctattgtgtc gcgcttttgc cttccggcat agttctgttt atgcttctgc
    41041 cagcgattat caaaacaatg aatttcacgg caggagtgag gcaatggaaa agaaaatcgg
    41101 ttttattggc tgcggcaata tgggaaaagc cattctcggc ggtctgattg ccagcggtca
    41161 ggtgcttcca gggcaaatct gggtatacac cccctccccg gataaagtcg ccgccctgca
    41221 tgaccagttc ggcatcaacg ccgcagaatc ggcgcaagaa gtggcgcaaa tcgccgacat
    41281 catttttgcc gccgttaaac ctggcatcat gattaaagtg cttagcgaaa tcacctccag
    41341 cctgaataaa gactctctgg tcgtttctat tgctgcaggt gtcacgctcg accagcttgc
    41401 ccgcgcgctg ggccatgacc ggaaaattat tcgcgccatg ccgaacactc ccgcgctggt
    41461 taatgccggg atgacctccg taacgccaaa cgcgctggta accccagaag ataccgctga
    41521 tgtgctgaat attttccgct gctttggcga agcggaagta attgctgagc cgatgatcca
    41581 cccggtggtc ggtgtgagcg gttcttcgcc agcctacgta tttatgttta tcgaagcgat
    41641 ggccgacgcc gccgtgctgg gcgggatgcc acgcgcccag gcgtataaat ttgccgctca
    41701 ggcggtaatg ggttccgcaa aaatggtgct ggaaacggga gaacatccgg gggcactgaa
    41761 agatatggtc tgctcaccgg gaggcaccac cattgaagcg gtacgcgtac tggaagagaa
    41821 aggcttccgt gccgcagtga tcgaagcgat gacgaagtgt atggaaaaat cagaaaaact
    41881 cagcaaatcc tgatgacttt cgccggacgt caggccgcca cttcggtgcg gttacgtccg
    41941 gctttctttg ctttgtaaag cgccaaatct gccgatttca accactcacg atagtgactc
    42001 atttgtgggt tcagcggcgc aacccccaca ctaatccgta aagttacctg tggcgtattc
    42061 ggcaagcgta acgtgtttag cccttcacgc acccgtaaca tggcggtaat ggcgctctca
    42121 gctggcgtac cggacatgat tactgcaaac tcatcgccgc caaaccgacc aatcacatcg
    42181 ctaccgcgca gggtaatttg taactgtcgg gtaagcgcca caatcgcttc atcgcccaca
    42241 tcatgacccc aggtatcgtt gatgctcttg aaatggtcga tatcgataat cagtaacgtt
    42301 gcatcgcgat tatgccgccg acagttatca aattcattgc gtaacatagt ttcccaatga
    42361 cgtcggttat acacgcccgt catgccgtcg cgggtactca tgacctgcaa cctgcgttta
    42421 tgttccgcca gtttggttgc cgtctggtag ctgacccagc caaacagcag aggataaacg
    42481 acaataatgg gaagggagag ccaccattcc agcggcgcac tattgaacga cacggtaatg
    42541 cccgtaagct cgagggtgac aaggcaggaa accaccatca acaccagacc cgcgacaaac
    42601 agacgggggc cgcctgcccc catcaaattc agacacataa tcatcaacat agcggtggaa
    42661 ggcagcacgt ttacgcccat tacgcctacc cacattcccg ctaatactgc atcggttttt
    42721 aagttgtaaa tttcccggct aagcggatcg acggccctgc tcgctatctg ccaggctaaa
    42781 tgcggccaga cgaacgccca gccgaccaac accagccacc accagcccgg cggcgggtgc
    42841 gaaaccagcg ttgaagcaat cggtaagaac atgccagcca ggccaaccgc acggggtagt
    42901 ctgacgcgac gggcgaagcg cagcccggac cgctgatgtt cgttttgagg agttaaagga
    42961 ggttcctccc catgcgccgc cgcttttttg aaaaagtttt catcattcat tatttttggg
    43021 aacattcaga aacaattttc ccaaattata gagacggggc taaaggcaga agtatgaaat
    43081 ttcgggtgag ccactttcgt agctcacccg ttagcactat caggtatcag gcggctttct
    43141 tgaggcaggc actcataaac ttattacgat catcaccttt cagagattgt tgcgttgctt
    43201 gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa acttttttca ccaggcgcag
    43261 acttgctgtt cttcaggcaa tcactcatgt aggtcttacg agcatcccct ttcaacgcct
    43321 gcgccgtcgc ctgctgatta caggaggtca tacgctgttg ttgtggggtt aaggttttct
    43381 cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag caaggtaacc agtaatgtta
    43441 ttttcatagc accatccctc ttcatgtttt aaccatgagc gtttgcgccc gtgatctgcc
    43501 attaagtctg gttgctaaca gcaaaaaaac cacccggcag cgaaaattca ctgccgggcg
    43561 cggttttatt tcagccccag agcggctttc atggtgtaga agagatcggt ctggtcggtc
    43621 agtccaacaa cattggcggc atgcgggcca tacgccgcaa tacgcaactg actgccggta
    43681 tgttcttgtg aatcctcttc agagttcccg taactcatca ccatcactgc gccatcttta
    43741 gtatttagcg cctgagtgag gcccggcgct ttggtatccg gcgcaacaac ctggctggcg
    43801 tgggcgtgat cagcggtgac tatgaccagc gtgttaccct cctttttagc gaattccagc
    43861 gcccgttgta cggcttcatc gagatcgacc gtctcgccaa tttgcccaca aggattcgca
    43921 gcgtgatcct gtttatcgat tgacgcacct tcaacttgca ggaaaaatcc tttctcattt
    43981 ttactcaaca attcaatggc tttgtcggtc atctgtgcca gggttggtac actgtcatta
    44041 cgttgcggat taggcgtaca ggtgactgcg ggcttatcga tattgccgtg gtacgttgct
    44101 ttcggcccta gccagcgcac tggcatattg ccgtcagcaa acaggccaag caggggtttt
    44161 tgctgattcg cttccgtcac cgaattcagt gaggcagcat cgttcaccaa ctgataacca
    44221 cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt caccagcggt tgccgtttca
    44281 gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac gagcgttaag cagctgttcg
    44341 gtaatcgatc cttttccgcc tttttccaga gcgttacccg gacatttttc actggtcgcg
    44401 ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca gcgcagcggg cgtggcatcc
    44461 tgcaactctg cggtagaaac gttaccggtc gccagacctg cggcttttgc catttccaga
    44521 atcgttgggt gatctttttc gtgaatatcg acgcccagcg cgccgttata ggttttgaca
    44581 ccggttgacc aggcggttgc tgatgcagcc gagtcggtga cgtagtccgg tttgccagtt
    44641 tttttattca gcgcatagtg agtgtattgc ccggtaagcg gtaaggcatc gataccttta
    44701 aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag taatttccga atcccccatc
    44761 ccatcgccaa tcagcaaaat aatatttttt gcaggtttat cgctaagaga atcacgcaga
    44821 gcggcggtat gatcacccgt taagcggcga gcaccgccgg gtgcagtaat atcgccctga
    44881 gcagcccggt tttccagaac aggcatttct ggagtccggg cttttgtcac aggggtaaac
    44941 agtaacgtta agagtgccag tgcaatagtg ctttgtttca ctttattttc tccatgtaca
    45001 aatacattaa aaaataaaaa caaagcgact ataagtctcg gccgtgacaa ctttatgaca
    45061 gctgttgaaa agattaactt tttactgacg aggatgcttc aataacttct ttacgtaatc
    45121 gcgcagcagc tccgtatcgt cgtcaggaat gctggcatcg ggctttacct cgtacagcgc
    45181 cccctctacc tgatcaatca accgctgttg gtcattttgc gccatattgc gaagcattgc
    45241 agtgacgata atctccaaag cttctacctg cgcacacagt tctttcgact cttcttcttt
    45301 ttgggcaagc ttaaataaca actcagcaat gagatttttc atgtctgtat ttccttatcc
    45361 aaagtatgga gaagttaaca ttatgtttca caacttcata gtgtctggaa gtattatttt
    45421 tatgacaaag gaaatacttt gcgcaaatat tttttgtttg attaccagcg aaacgtttct
    45481 cttatttaaa taattaccca cacaaagtat aggcttttaa ttatcaacct ttattataaa
    45541 taaaagttat gaaatatatt ctcttcgtta acttataatt aagagaaaaa aactcaccgc
    45601 atggccattt aagccaggaa taaattagct tcccgttcat catttttgtg atcttgcgca
    45661 catctttgag ataacgcact ttcttcacca gtacgttgca cgctttgtca gcagaagatt
    45721 gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt atgcactttg
    45781 tgttagacgg gataggtttt taagatggaa aaactgcggg taggaatcgt ttttggtggt
    45841 aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga tgccattgat
    45901 aaaagtcgct tcgacgttgt gctgctgggc attgataaac aagggcaatg gcacgtcagc
    45961 gatgccagca attatctgct aaatgcagac gatcctgccc atattgcgtt gcgcccttcg
    46021 gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc agcttatcga cgcgcaaaac
    46081 ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg tccacggtac gctgggcgaa
    46141 gatggttcct tgcagggaat gctgcgggtc gccaatttac cgtttgtagg ttctgatgtt
    46201 ctggcttcag cagcctgtat ggataaagat gtcaccaaac gtctgctacg cgatgccggg
    46261 ctgaacattg cgccatttat taccctgacg cgcgctaatc gtcacaacat cagttttgcc
    46321 gaagtggagt ctaaactggg gttaccactg tttgtaaaac cggctaatca gggctcttct
    46381 gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa ttgccgtcga tctggcgttc
    46441 gagttcgatc ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat cgaatgcgca
    46501 gttctgggca acgacaatcc gcaagccagc acctgtggcg agatcgtact caccagcgat
    46561 ttctatgcct acgacaccaa gtacattgac gaagatggcg cgaaagtggt agttccggca
    46621 gccattgcac cagagatcaa cgataagatc cgggcgattg ccgttcaggc ttatcagacg
    46681 ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa ccccagagaa cgaagtggtg
    46741 atcaacgaga tcaacacact gcctggcttc accaacatca gtatgtatcc gaagctgtgg
    46801 caagccagcg gtctgggtta caccgatctg atcacacgtt tgattgagct ggcgctggag
    46861 cgtcacgctg cggataacgc attgaaaacc acaatgtaat attttaacgc ccgataatat
    46921 tcttattggg cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc cgccagccag
    46981 aaactaatca cccacgtcac cagaccgacg gcataggtct gccaaccttt tgcttcaaat
    47041 cccagtaacc ccaccacgcc attaagaatg aatataaggc caatggcgaa cgcatagtag
    47101 tgccagtcac ggcggatttt tacaggcaga ttcatggcag ctccgttaac gaaaaacaac
    47161 atccttgcat attctgtaac aaccgtctgt ggtgtagaca acattttcgt aattgttaat
    47221 aaatttaact taaggttgcc attttgtgac tcacaacgaa gattcacgct tcgggaatat
    47281 tcccaacatc aatttaccat gattctgata ttgacaatct aaatgacctg tcagacttgc
    47341 cataagccgc tggtatcaca ccagaagaga agcagatgac ttccggaggt ttttatggct
    47401 gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc ttcaacgcct
    47461 ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt gggctggggc gcaattgctg
    47521 aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca ggaaacaggt
    47581 cgcccacggg tggaaattgg tttaggtgtc ggcaccattt tcgggctgat cccgttttta
    47641 gtaggctgcc tcatttttgc agtggtggcg ctatggctgc actggcgaca tcgccgccag
    47701 taagatttac gttgccagat acggcctaaa acgccttatc tggcatgaca ttcctgtttg
    47761 atcactgttt catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac caggccgtag
    47821 tcaggttgcg ggtgattttc gggctttcca gttgaatgcc aggcaacatt tcgcggggta
    47881 gtgatttacc cgttttcgct tcggcaagtt gataaacttt cttgtacagc gccgtctcct
    47941 caaaagagaa gctgtcacct ttctctaact gacggcgaat ttcgctgtcg ctcatcccca
    48001 gttttcccgc cagtttgcgc gttgccagtt cggttttccc gggttcttta ctgtcgtaac
    48061 gaattaaatc accgtccagt gccagcttca cgccgctggc cttactgact gcgttctgaa
    48121 acgcggcatt acggctggcg taccagccag cgttaaaatc agcaaagcgg tatatcggtg
    48181 cgctatagct ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac ccgccgcggc
    48241 gactgaagac ttcctgacga actgtaccgt ccattttcca cggataccct ttggtatgct
    48301 gttcggcaaa agcaatgctg acctgcatcg gcccaccggt gcgcacagga ttaagcgaac
    48361 caaacagcgt ctgccccatt ggtaccatgc tgatcagatc gtcaaaaatc gcgctcaatt
    48421 gcttttctgt acgcaccgaa tctaaccgtt cgctataact tttgccgttt ggcgatttga
    48481 tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat ccgttcagca cgacggtcaa
    48541 tctcttgcca ggcgatcttg cttaagcccg gaacggccgg atctgcctga taatttgact
    48601 cctgctgcgc caccgccagc accgagcaga cgttttccag cgtcggtgcc agcccctgac
    48661 tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg ctcgcaggca
    48721 tcttctgccg cacgacgctc gccacatcta tcgctttttc gccctttttc aacggttgcg
    48781 gagcctgact actacacccc gctaaaacca gaaccgccaa caatgacagc gaagatagag
    48841 gattgacgcg cgacattact tctccttttt agctcaaggt atgggttact tcctgaattt
    48901 tgtcaccatc cagttcatgt tcaaagctgc gcagacgttt gtagatagac atcaactcaa
    48961 ccagtgtggt ccatgagtta atcaggtact ggaaagcacc gcgaacctga ccaaaaacgt
    49021 tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac ggaaacagca
    49081 agaacaaacc gaaaacgtta tcgacctgca aatagagaat gcgggcgatg ttgaaataca
    49141 tatagtgaaa atagaggcgg aaatagtttt tccgcacggc gctaaacagc tcgcgtaccg
    49201 taggcggcgt cgcacgcgtg gcatcgtctt caccataaac cagctcttta cggtaggcgg
    49261 cctctactcg ctggttttta aactccagcc ccggcagttt gatccctacc actgccagca
    49321 atccggtccc catcagcgac cagacgatag cggcaatcac cagaccatac ggaatgtgcc
    49381 cgataatcgg cagctccggc acatgcgtgg agagcgttac cagcaccggc aggaaggcga
    49441 tcaacgtcat gatggcgttg ataaaactga cgcccatatt ctccagcgtt gaagcaaaac
    49501 gcatggtgtc ttcctgcaca cgctgtgcgg ccccttcgat atgacgcagt tgttgccagt
    49561 tcgccatgta atattcgttc atcgccgtac gccagcggaa cacgtagtga ctgacaaaga
    49621 agttgttcag cacactgatc accacagcga tcagcgcaat ccccagaaag acgcccactt
    49681 cgcggtaaaa ttgttcgata gtgactttat gcggcgaact tagcgcggtt tgaatcagat
    49741 catagaacgg tgcataccag gcgttgacgg cgaccccgac ttccaccaaa aaccaggtga
    49801 cgaagatgat cagtgcagta ccgagtattg accagtattg ccaacggtgc gggctgtaga
    49861 taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa aaaatcagga
    49921 aatccaacga ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg ccagtgatac
    49981 gcgccaccca ctctccccca ccggcttgcc agaagataac ggcaatcaat gcccaaacaa
    50041 aggccgagag aaaaaacgct cccggctttg ggaaaaaaga cttaaacata tgcactcctg
    50101 ctaacttctt atcgttttaa caactgctgt gtattaaccg catgaccgct acaaagcgtc
    50161 agccaaatga aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc agcctgacca
    50221 taagaattgt ttcgacggat ttcacaaacc aaagattgtt tacataagcg gattaacacg
    50281 cgcaggatca caaatgcagg cattggtgag caggaaaaag atcgtgttta cgcgccgtta
    50341 cgtttttaac actattattg cactaattcg ccctttgcaa tccatcaatg agtagtataa
    50401 atacgctcag ttaccttcat tcaatctatg gacaccaccg ttgaaacgta gtctgctttt
    50461 ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc cgatcactga
    50521 accggagttt gcctctgata ttgtcgatcg ttatgccgat catatttttt acggcagcgg
    50581 tgccacgggg atggcgctgg ttgttatcga cggtaaccag cgcgtctttc gcagttatgg
    50641 cgaaacgcga cctggtaata acgttcgccc gcagctggat tcggtcgtgc gtattgcttc
    50701 cctcaccaag ctaatgacca gtgaaatgct ggtgaaattg ctcgaccagg ggaccgtgaa
    50761 gttaaacgat ccgctaagta aatatgctcc gccaggcgca cgcgtgccaa cctacaatgg
    50821 gacgcccatt accctagtga atctggcaac ccataccagc gccctgcccc gtgaacagcc
    50881 cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg cgcgagcaac gctggaaata
    50941 cctttctacg gcgaagctga aagccgcgcc aggcagtcag gcggcgtact ctaaccttgc
    51001 gttcgacctg ctggcagatg cactggcgaa tgcgtccggc aagccttata cccagttgtt
    51061 tgaagagcag attacccgtc cattggggat gaaagacacc acctacaccc cctcaccgga
    51121 tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt ccgtgcaata acacgctggc
    51181 ggcaattggc agcggcggcg tctattccac gcctggcgat atgatgcgct ggatgcagca
    51241 gtatttgtca tctgattttt atcagcgcag taaccaggca gatcgcatgc agacgttgat
    51301 ttatcagcgc gcgcagttta cgaaagtgat tggcatggat gtccccggca aagcggatgc
    51361 gcttggttta ggctgggtat atatggcccc gaaagagggt cgtccgggga ttattcagaa
    51421 gacaggcggt ggtggcggtt tcattaccta tatggcgatg atcccgcaga agaatattgg
    51481 cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga gcgatggcat
    51541 caatgatctg gtgaccgagc taagcgggaa taaaccgttg gttatccccg catcctgatt
    51601 aatactctga aggcaaacgg ttgatagcga ccagtttgcc tttattcatt tcgatatagc
    51661 cgcctttacg caaagcagcg agaacttctg cgacgacaga acgcgatatg cgtgtacgtt
    51721 gatggatata attcatcaca ccaatacgtg atcgcaattc ttcattccag tcaatcattg
    51781 ataataatgt tgcgcggatt tgttcgtagg aattatgccc aatgagctgc acgtcgcgta
    51841 attccagaat tctattttgc caggctaacc agtaaaaagc gtctcgccag agttgatttt
    51901 gttcaataag cgtaatggtt tgtttggctg gtagatgata tcccgtacaa tttccttctg
    51961 atattaattt gtatggtata tcgtttttca ttaaaccatc agccagcccc ataatataag
    52021 gagcctgggt aataccgata agtacgtttt cttctctacg cagagagata acgccctcta
    52081 aaataacgaa agtatcttcg ttattgacat cactggaaaa tataacttgc ttttcattat
    52141 taaactcgaa gcgcgtaccg tatctggaca aacatttatc gagcttacca aattcctgaa
    52201 gaggtttaac tacagataac atttgcgcgt cctttgcagt aatgcccgtc aaatccttga
    52261 cgggcattat ttagattaaa ttaccagaat ttcttcggag tgaagaatat taccaggtat
    52321 atttaacacc cacgttcgcg gaccagtctt gatctacgtc accaccaccg aggtagttag
    52381 catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga ccaacacgta
    52441 ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta tcgttgttag
    52501 agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctggtct tcgctgtagg
    52561 tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa gactgaccgt
    52621 caactttcat gtcgttgctc agctggtagt catcaccaga ctggaacaga ccagaaatgc
    52681 tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg gctttcaaac
    52741 cgaagcccca agcgtcggag ttggtgctac cgtcaacgta agtaccgttg ctcatggttg
    52801 cagacaggtc gttgttgaag tgagagtaac tcaagctacc atcaacaaag acgttgttcg
    52861 cgaagtgagc agaagagtag atgtaggcag tctggctgtc ttgatccacc tgaccagaac
    52921 ggtcattcat gtcaccttta gcgaagcctg cagccgcacc gacgatccac ttagcgttgt
    52981 taccgtcaat tttggtatca acaccgacca tgatgccgtt aacatcctga tcatagttga
    53041 tggtgccgtt gtcgccgttg aagttaccac cgaagtagct tacccatgcg ccgccgttat
    53101 cagccaggcc atgacgagag ttggtcagac gagtaccaac ggtgtcttgt tccaggttcc
    53161 agatattggt gttcgcagac gggatgctca gcgccatgtt agcgtagtcg gtcagctcca
    53221 tctgttgcag aacaacggtg ttaccgcgct gttcagcctg ataggtgtat gcacccaggt
    53281 cagctttgtt agcagcagag aaggtcgcgt tggtgttgac gtcgtttacg tagataattt
    53341 ctttatcttt gtaatcagcg atagcgccag cgcctgttgc gttgtcgata cgaactttgt
    53401 agttaccggt tgcagcaaca acgttgtcaa cagagctgtt atccagttcg tcgccgttgt
    53461 tcatgtcacc gttaccgttg atagtcagat gaccatcaga gttcatagca acaataccgt
    53521 agccgtagtt agacttggaa gtatcccagg tacggtcgtt caccaaatca gcgttcagaa
    53581 cataatcgct gctgtggata tcgaatacac cagacactac gttgccgtta tggtcagcga
    53641 tgttgccgct ggtcagttcc aaagtatcag tctggaacgg attatcatcg aactggtcta
    53701 cattaatatc cagtaaacca ccgttagtca cagttattgt atttgctaac agcgacggag
    53761 tgtaataacc attatagata cttgaattgt catcactaga cagcgctaca gtactgtagc
    53821 tatcaactgt cagatgatca gttccaacat aaccttcatc tccaatgttc aaagaagaac
    53881 cattggtcag agcgatgcta tcagaaatca agctagattc agacacatta acctgagagc
    53941 cattattcac agctaaagta tcaatgtttg aagcgttaac ggtattccat tcagaaccat
    54001 tattcagagt gacgttaaac acaccgctct ggaatacttg atcatcaatc gtacgacccc
    54061 aagcatttgc ttctgttaca acgcccggat acaggctatt acctgtaaca tcataccagt
    54121 cagtcgaagc agtagattca tcatcaacat ttgctgatgt agtagctgag cctacccact
    54181 tagaaccatt atccagagtg atattcagtt catcaacatt aagtgagtca tcagcccagc
    54241 caccattggt gtccagtacg ccatcggcat tactgtcatg accttgagga tagaagtttg
    54301 cgttgaaagt acttgcgaaa acaacatcac ccagcagagt agaattgctg aaataaacat
    54361 tgtttttcat ggagtaattt gaagccgcac tatcactgaa atacagagct acatcattag
    54421 cttcgccgtt accagagtaa tcactaggtt ccgcagagtt accatagaaa ccactgtctt
    54481 ccagagtagt tacagaacct gaagtcactg tggaatcggt aactgagata ttgttatccc
    54541 aagtatgaga ttcagtagtg taagtattgt ttacggtatt accctgagtc aggctgatac
    54601 cagcaacgcg ggagttgtta gaaatattga tgttactttc gacatcaaga ttcactgcca
    54661 gcccaaggcc aacaccaccg tttgcaaaca aaccggcgta ttcttcacct tcaccagctg
    54721 gggtatggaa ggtttcgtta gtatatttgg taacgtcacc gtccagatat gaatcagtga
    54781 agtataaacc ttcataatcg tcatcaattg tagaattagc aatatttaaa gtgaatacat
    54841 caccatcatg ccaattatca tcaattacac gatcagtacc gtattcagag aactgataga
    54901 cataatgacc atcactgctt tcacccatag gcagcattga agtgattgaa ccatgaatca
    54961 gggagttgct gatgttcaac tggtttgttg ttccatcagt aaagtcatga ttcaggtaat
    55021 atgtagagat agtcccgtta acagtgtcac cattaaaggt ggtatcgaaa tcaccgtttg
    55081 tgttgttata gtagacaaag ccactataat tcacatagtt accatctaca gtacccggtg
    55141 catccatatt gctccagtca agagcggtag ttggattgtc gtaataagtc caggtgtcat
    55201 aagtagttgc attagcctga gaggtgatag ccagagtgca agccaatgct aattgtgata
    55261 ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg taataagttg
    55321 atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaatga ttatgctgcg
    55381 ttacagacga atggttcaat tattctttcc cctaagtccg aatgtggaca tatataacct
    55441 atggagaaaa acacgcatca aattgataaa agggcgatat ttcaacatgt tgcagataat
    55501 gttatttttg atacatacgc g
//
LOCUS       DAZFYC010000018        52875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFYC010000018 DAZFYC010000000
VERSION     DAZFYC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.018"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..534
                     /gene="cmtB"
                     /locus_tag="ACHKW5_001242"
     CDS             91..534
                     /gene="cmtB"
                     /locus_tag="ACHKW5_001242"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO7510674.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            562..1950
                     /gene="cmtA"
                     /locus_tag="ACHKW5_001243"
     CDS             562..1950
                     /gene="cmtA"
                     /locus_tag="ACHKW5_001243"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO7510675.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNLGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1965..3242
                     /gene="yggP"
                     /locus_tag="ACHKW5_001244"
     CDS             1965..3242
                     /gene="yggP"
                     /locus_tag="ACHKW5_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7510676.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3239..4204
                     /gene="glpX"
                     /locus_tag="ACHKW5_001245"
     CDS             3239..4204
                     /gene="glpX"
                     /locus_tag="ACHKW5_001245"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7510677.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDTYMKKLVVNRLAAGAIDLSLPLADNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4226..4735
                     /gene="fumE"
                     /locus_tag="ACHKW5_001246"
     CDS             4226..4735
                     /gene="fumE"
                     /locus_tag="ACHKW5_001246"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO7510678.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4732..5445
                     /locus_tag="ACHKW5_001247"
     CDS             4732..5445
                     /locus_tag="ACHKW5_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO7510679.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5417..6087)
                     /locus_tag="ACHKW5_001248"
                     /pseudo
     CDS             complement(5417..6087)
                     /locus_tag="ACHKW5_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(6081..6758)
                     /locus_tag="ACHKW5_001249"
     CDS             complement(6081..6758)
                     /locus_tag="ACHKW5_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7510680.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6746..7453)
                     /locus_tag="ACHKW5_001250"
     CDS             complement(6746..7453)
                     /locus_tag="ACHKW5_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO7510681.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAIRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7454..8032)
                     /locus_tag="ACHKW5_001251"
     CDS             complement(7454..8032)
                     /locus_tag="ACHKW5_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO7510682.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8055..8486)
                     /locus_tag="ACHKW5_001252"
     CDS             complement(8055..8486)
                     /locus_tag="ACHKW5_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO7510683.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPDCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8858..9877
                     /gene="epd"
                     /locus_tag="ACHKW5_001253"
     CDS             8858..9877
                     /gene="epd"
                     /locus_tag="ACHKW5_001253"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO7510684.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9927..11090
                     /gene="pgk"
                     /locus_tag="ACHKW5_001254"
     CDS             9927..11090
                     /gene="pgk"
                     /locus_tag="ACHKW5_001254"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO7510685.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11305..12384
                     /gene="fbaA"
                     /locus_tag="ACHKW5_001255"
     CDS             11305..12384
                     /gene="fbaA"
                     /locus_tag="ACHKW5_001255"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO7510686.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12448..12546)
                     /locus_tag="ACHKW5_001256"
                     /pseudo
     CDS             complement(12448..12546)
                     /locus_tag="ACHKW5_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12742..13602
                     /gene="mscS"
                     /locus_tag="ACHKW5_001257"
     CDS             12742..13602
                     /gene="mscS"
                     /locus_tag="ACHKW5_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO7510687.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13741..14376
                     /gene="argO"
                     /locus_tag="ACHKW5_001258"
     CDS             13741..14376
                     /gene="argO"
                     /locus_tag="ACHKW5_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO7510688.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14469..15209
                     /gene="yggE"
                     /locus_tag="ACHKW5_001259"
     CDS             14469..15209
                     /gene="yggE"
                     /locus_tag="ACHKW5_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO7510689.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15376..16272
                     /gene="ygfI"
                     /locus_tag="ACHKW5_001260"
     CDS             15376..16272
                     /gene="ygfI"
                     /locus_tag="ACHKW5_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7510690.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMSASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16269..17747)
                     /gene="scpC"
                     /locus_tag="ACHKW5_001261"
     CDS             complement(16269..17747)
                     /gene="scpC"
                     /locus_tag="ACHKW5_001261"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO7510691.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17771..18556)
                     /gene="scpB"
                     /locus_tag="ACHKW5_001262"
     CDS             complement(17771..18556)
                     /gene="scpB"
                     /locus_tag="ACHKW5_001262"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO7510692.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHAIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18567..19562)
                     /gene="meaB"
                     /locus_tag="ACHKW5_001263"
     CDS             complement(18567..19562)
                     /gene="meaB"
                     /locus_tag="ACHKW5_001263"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO7510693.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19555..21699)
                     /gene="scpA"
                     /locus_tag="ACHKW5_001264"
     CDS             complement(19555..21699)
                     /gene="scpA"
                     /locus_tag="ACHKW5_001264"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO7510694.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRIVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21903..22796)
                     /gene="argP"
                     /locus_tag="ACHKW5_001265"
     CDS             complement(21903..22796)
                     /gene="argP"
                     /locus_tag="ACHKW5_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO7510695.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22938..23123
                     /locus_tag="ACHKW5_001266"
     CDS             22938..23123
                     /locus_tag="ACHKW5_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510696.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23179..23838
                     /gene="rpiA"
                     /locus_tag="ACHKW5_001267"
     CDS             23179..23838
                     /gene="rpiA"
                     /locus_tag="ACHKW5_001267"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO7510697.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24094..25326
                     /gene="serA"
                     /locus_tag="ACHKW5_001268"
     CDS             24094..25326
                     /gene="serA"
                     /locus_tag="ACHKW5_001268"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO7510698.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25555..25614
                     /gene="ibsC"
                     /locus_tag="ACHKW5_001269"
     CDS             25555..25614
                     /gene="ibsC"
                     /locus_tag="ACHKW5_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO7510699.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25947..26006
                     /locus_tag="ACHKW5_001270"
     CDS             25947..26006
                     /locus_tag="ACHKW5_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7510700.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26107..26709)
                     /gene="fau"
                     /locus_tag="ACHKW5_001271"
     CDS             complement(26107..26709)
                     /gene="fau"
                     /locus_tag="ACHKW5_001271"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO7510701.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26730..26913)
                     /gene="ssrS"
                     /locus_tag="ACHKW5_001272"
     ncRNA           complement(26730..26913)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKW5_001272"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26955..27284)
                     /gene="zapA"
                     /locus_tag="ACHKW5_001273"
     CDS             complement(26955..27284)
                     /gene="zapA"
                     /locus_tag="ACHKW5_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7510702.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27452..28030
                     /gene="ygfB"
                     /locus_tag="ACHKW5_001274"
     CDS             27452..28030
                     /gene="ygfB"
                     /locus_tag="ACHKW5_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO7510703.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28056..29381
                     /gene="pepP"
                     /locus_tag="ACHKW5_001275"
     CDS             28056..29381
                     /gene="pepP"
                     /locus_tag="ACHKW5_001275"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO7510704.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29378..30556
                     /gene="ubiH"
                     /locus_tag="ACHKW5_001276"
                     /gene_synonym="visB"
     CDS             29378..30556
                     /gene="ubiH"
                     /locus_tag="ACHKW5_001276"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO7510705.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGETLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30579..31781
                     /gene="ubiI"
                     /locus_tag="ACHKW5_001277"
                     /gene_synonym="visC"
     CDS             30579..31781
                     /gene="ubiI"
                     /locus_tag="ACHKW5_001277"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO7510706.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLVADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31855..31938)
                     /locus_tag="ACHKW5_001278"
     CDS             complement(31855..31938)
                     /locus_tag="ACHKW5_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510707.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            32228..33322
                     /gene="gcvT"
                     /locus_tag="ACHKW5_001279"
     CDS             32228..33322
                     /gene="gcvT"
                     /locus_tag="ACHKW5_001279"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO7510708.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33346..33735
                     /gene="gcvH"
                     /locus_tag="ACHKW5_001280"
     CDS             33346..33735
                     /gene="gcvH"
                     /locus_tag="ACHKW5_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO7510709.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTFTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33854..36727
                     /gene="gcvP"
                     /locus_tag="ACHKW5_001281"
     CDS             33854..36727
                     /gene="gcvP"
                     /locus_tag="ACHKW5_001281"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO7510710.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            36988..37731
                     /gene="ygfF"
                     /locus_tag="ACHKW5_001282"
     CDS             36988..37731
                     /gene="ygfF"
                     /locus_tag="ACHKW5_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7510711.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37788..39221)
                     /gene="bglA"
                     /locus_tag="ACHKW5_001283"
     CDS             complement(37788..39221)
                     /gene="bglA"
                     /locus_tag="ACHKW5_001283"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO7510712.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39266..39577
                     /gene="yqfB"
                     /locus_tag="ACHKW5_001284"
     CDS             39266..39577
                     /gene="yqfB"
                     /locus_tag="ACHKW5_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO7510713.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39741..40400
                     /gene="yqfA"
                     /locus_tag="ACHKW5_001285"
     CDS             39741..40400
                     /gene="yqfA"
                     /locus_tag="ACHKW5_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO7510714.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40478..41458)
                     /gene="ygfZ"
                     /locus_tag="ACHKW5_001286"
     CDS             complement(40478..41458)
                     /gene="ygfZ"
                     /locus_tag="ACHKW5_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO7510715.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41701..41967
                     /gene="sdhE"
                     /locus_tag="ACHKW5_001287"
     CDS             41701..41967
                     /gene="sdhE"
                     /locus_tag="ACHKW5_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO7510716.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            41948..42355
                     /gene="ygfX"
                     /locus_tag="ACHKW5_001288"
     CDS             41948..42355
                     /gene="ygfX"
                     /locus_tag="ACHKW5_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO7510717.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42395..42916)
                     /gene="fldB"
                     /locus_tag="ACHKW5_001289"
     CDS             complement(42395..42916)
                     /gene="fldB"
                     /locus_tag="ACHKW5_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO7510718.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43028..43924
                     /gene="xerD"
                     /locus_tag="ACHKW5_001290"
     CDS             43028..43924
                     /gene="xerD"
                     /locus_tag="ACHKW5_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO7510719.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43949..44659
                     /gene="dsbC"
                     /locus_tag="ACHKW5_001291"
     CDS             43949..44659
                     /gene="dsbC"
                     /locus_tag="ACHKW5_001291"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO7510720.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44665..46398
                     /gene="recJ"
                     /locus_tag="ACHKW5_001292"
     CDS             44665..46398
                     /gene="recJ"
                     /locus_tag="ACHKW5_001292"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO7510721.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46489..47587
                     /gene="prfB"
                     /locus_tag="ACHKW5_001293"
     CDS             join(46489..46563,46565..47587)
                     /gene="prfB"
                     /locus_tag="ACHKW5_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO7510722.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47597..49114
                     /gene="lysS"
                     /locus_tag="ACHKW5_001294"
     CDS             47597..49114
                     /gene="lysS"
                     /locus_tag="ACHKW5_001294"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7510723.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49157..49705)
                     /gene="idi"
                     /locus_tag="ACHKW5_001295"
     CDS             complement(49157..49705)
                     /gene="idi"
                     /locus_tag="ACHKW5_001295"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO7510724.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            49760..49831
                     /locus_tag="ACHKW5_001296"
     CDS             49760..49831
                     /locus_tag="ACHKW5_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510725.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(49828..49953)
                     /gene="yqfG"
                     /locus_tag="ACHKW5_001297"
     CDS             complement(49828..49953)
                     /gene="yqfG"
                     /locus_tag="ACHKW5_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO7510726.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49955..51403)
                     /gene="uacT"
                     /locus_tag="ACHKW5_001298"
     CDS             complement(49955..51403)
                     /gene="uacT"
                     /locus_tag="ACHKW5_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO7510727.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVCMWLLMRKLKKNEHGE"
     gene            51839..>52875
                     /locus_tag="ACHKW5_001299"
     CDS             51839..>52875
                     /locus_tag="ACHKW5_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7510728.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIG"
BASE COUNT        12795 a        13156 c        14216 g        12708 t
ORIGIN      
        1 ggtttccctg ctcaattgct ttcattaaac cgcacgagta ttttattttt accgccggac
       61 ggtaccctaa aaataaaata aggataattt atgcggctta gtgactattt tccagaatca
      121 tcaatctcag ttatacattc agcaaaagat tggcaggaag ctatcgattt ctcgatggta
      181 tcattgctgg ataaaaacta tatcagcgag aattacattc aggcaattaa agattccacc
      241 attaacaatg gcccttatta tattctcgca ccaggcgtgg caatgcctca tgcgcgaccg
      301 gaatgtgggg cgcttaaaac cgggatgtct ttgacattac ttgaacaagg tgtttatttt
      361 ccggggaatg acgaaccaat taaattactc atcggactct ctgctgccga tgccgattcg
      421 cacattggcg ctattcaggc gttaagtgaa ttactgtgcg aagaagaaat actcgaacaa
      481 ctcttaacag catcatcaga aaaacaatta gcggacatta tcagccgcgg ataattactc
      541 tctcctctac aggataatat tatggaaaac aagtctgctc gtgcaaaggt ccaggctttt
      601 gggggctttt tgactgcaat ggtcatcccc aatataggtg cttttattgc ctggggcttc
      661 ataactgcgt tatttattcc caccggttgg ctgccgaatg aacatttcgc caaaattgtc
      721 ggcccgatga ttacctattt attgcccgtg atgattggtt ctactggtgg tcatctggtc
      781 ggcggtaaac gcggggcggt catgggcgga ataggtacta ttggtgtgat cgttggcgca
      841 gagatcccga tgttccttgg ctcaatgatc atggggccgc tcggtgggtt ggtcataaaa
      901 tatgtcgata aggcactgga aaaacgcata cctgccggtt ttgagatggt tatcaataac
      961 ttctcattag gtatcgcggg gatgctcctt tgtctgctgg gttttgaagt gatcggcccg
     1021 gcggtgttaa ttgccaatac tttcgtcaaa gagtgtatcg aggcgctggt acatgcgggt
     1081 tatctgcctc tgctgtcagt catcaatgaa ccggcgaaag tgcttttcct taataatgcg
     1141 atcgatcagg gcgtctatta tccgctggga atgcaacagg cttcggttaa cggtaaatcc
     1201 atcttcttta tggtggcctc taacctaggt ccgggcctgg ggctgctgct ggcgtttacc
     1261 ttgtttggta aagggatgag taaacgttct gcgcccgggg cgatgattat tcacttcctc
     1321 ggtgggatcc acgaactgta tttcccatat gtgctgatga agccgctgac tattattgcc
     1381 atgattgcgg gcggtatgtc tggtacctgg atgtttaact tactggacgg tggtctggtg
     1441 gctggcccaa gtccggggtc tatctttgct tacctggcac tgacgccgaa aggctcgttc
     1501 ctggcgacaa ttgccggtgt tacggtaggt accctggtgt cctttgctat tacttcgctg
     1561 atactgaaga tggaaaaaac ggtggaaacg gagagcgaag atgagtttgc tcagtcagcc
     1621 aatgcggtta aggcgatgaa acaagagggt gcattctcgt taagcagggt taagcgtatc
     1681 gcctttgttt gcgatgcggg gatgggctcc agtgcgatgg gcgcgaccac cttccgtaaa
     1741 cgcctggaaa aagcggggct ggcaatagaa gtaaaacatt acgcgataga aaacgtgcct
     1801 gcggatgccg atatcgtcgt cactcacgcc agtctggaag ggcgcgtgaa acgtgtgacg
     1861 gataaaccgc tgatattgat taataactat attggcgatc caaaactcga cactttattt
     1921 aatcaattaa ccgccgaaca taaacactga ttacagaggt aaaaatgaaa accaaagttg
     1981 ctgctattta tggcaagcgg gatgtccgtc tgcgcgaatt tgaactgcca gaaattaccg
     2041 ataatgaact gttagtgagt gtaatttctg acagcgtctg tttatcgacc tggaaagcgg
     2101 cgttactcgg tagtgaacat aaacgcgtac ccgacgattt agaaaatcat ccggtcatta
     2161 ccgggcatga atgtgccggg gttattgtcg aagtgggtaa aaatctcact ggcaaatata
     2221 aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt accaagcgga tattcagcag
     2281 gctacagcta cgaatatttt ggcggtaacg ccacctatat gattattccc gaaatcgcca
     2341 ttaatttggg ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg tcgctggcag
     2401 agcctatgtg ctgcattatt ggtgcttatc atgccaatta tcacaccacg caatatgttt
     2461 atgagcatcg catgggcgtc aaacctggcg gcaatattgc actgctggcg tgtgcgggtc
     2521 cgatgggcat tggcgctatc gattacgcca ttaacggtgg catacaaccg tcacgggtgg
     2581 tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca gaagctgctg ccggtggatc
     2641 tggcggccag taaaggcatt gagctggtgt atgtgaatac caaagggatg agcgatccgg
     2701 tccagacgct gcgggcgctg acaggagatg tcgggttcga tgacattttt gtttatgcgg
     2761 cggtgcctgc tgtcgttgag atggctgacg aattactggc ggaagatggc tgtctgaact
     2821 tctttgccgg gccgacggat aaaaacttca aagtgccgtt taatttctac aacgtccatt
     2881 acaacagcac gcacgtagtc ggtacatccg gcggttcaac ggacgacatg aaagaggcga
     2941 ttgcgttaag cgccacgggg cagttgcagc cgtcctttat ggtcacgcat atcggtgggc
     3001 tggatgcggt gccagatacc gtgctcaatc tgccggatat ccctggcggt aaaaaactca
     3061 tttataacgg cgtgaccatg ccgctcactg ccattgccga ttttgccgaa aaaggcaaaa
     3121 ccgatccgct gtttaaagag ttggcgcggc tggttgagga aacgcacggc atctggaatg
     3181 aacaggccga gaaatatctg ctggcacaat ttggcgttga tatcggggag gccgcgcaat
     3241 gatgtccctg gcgtggccat tatttcgcgt tacggaacag gcagcgttgg ctgcctggcc
     3301 gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg gcggtcaccg cgatgcgcca
     3361 ggcattaaac gacgtcgctt ttcgtggacg agtggttatc ggcgaaggag aaattgacca
     3421 tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc gatgggccag aagtcgatat
     3481 cgcggttgac ccgattgaag gcacgcggat ggtagcgatg gggcagagca atgcgctggc
     3541 ggtcatggct ttcgcccctc gagatagcct gttacatgcg cccgatacgt atatgaaaaa
     3601 gctggttgtt aatcgactgg cggcgggcgc gattgatctg tctttgcctc tggcggacaa
     3661 cttgcgcaac gtggcgaaag cgttaggtaa accgctggac aagctgcgca tggttacgct
     3721 ggataaaccg cgcctgagtg ccgcgattga agaagcaaca cagctgggcg tgaaggtttt
     3781 cgctctgccg gatggcgatg tcgccgccag cgtgctgacc tgctggcagg ataatcctta
     3841 cgacgtgatg tacaccatcg gcggtgcgcc agaaggcgtg atttctgcct gtgccgttaa
     3901 agcattaggt ggggatatgc aggccgagct gattgacttt tgtcaggcta aaggggatta
     3961 cacggaaaat cggcagattg cagagcagga gcgcaagcgt tgtaaggcaa tgggcgttga
     4021 cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg aacgatatcc tctttagcgc
     4081 tacgggcgtg acgggcggcg agttggtgaa cggtatccaa cagacggcga atggggtgcg
     4141 gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt aatataatag actccctgca
     4201 ttgatggtga tctatcggaa gaccaatggc gacgctgaca gaagatgatg tgcttgagca
     4261 actggatgca caggacaatt tattttcatt tatgaaaact gcgcattcta ttttgctcca
     4321 ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac gatgaagaga tcgtcgaata
     4381 tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac gatattgatg ttgcgctgcg
     4441 tttgatttat gcgctgggga aaatggataa atggctgtat gccgatatca cgcacttttc
     4501 ccagtactgg cattacctga acgaacagga tgaaacgccc ggatttgccg atgacatgac
     4561 ctgggatttt atcagcaacg tcaatagcat aacctgcaat gcgacgctct atgacgcgtt
     4621 aaaagcgatg aagttcgccg atttcgccgt ctggtcagag gcgcgtttta gcggaatggt
     4681 caaaacggcg ctgacgctgg cagtaacgac caccttaaag gaattaacgc cgtgaaaatt
     4741 gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc atgatgaaga gatagaacgc
     4801 gtgcataaac cgttgctgcg tatgctggcg gccttacaga cggtaagtcc gcagcgacgt
     4861 acggtggttt ttctttgcgc tccgccgggg acgggtaaat ctaccttaac taccttctgg
     4921 gaatatctcg cgcaacaaga ccctgaatta cccgctatcc agacgctccc gatggatggt
     4981 tttcaccatt acaatagctg gctggatgcg catcaattgc gccccttcaa aggcgcacca
     5041 gagacatttg acgttgcgaa actggcggaa aatctgtgcc aggtagtgga aggggattgt
     5101 acgtggccgc agtatgatcg acaaaagcat gatcctgttg aagatgcgtt gcacgttacc
     5161 gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg acgacgagaa gtggtgccag
     5221 cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc cagctactgc gttgcgggaa
     5281 cgactggtag ggcgcaagct ggcgggaggg gtatcgctgg cagacgcaga agctttttat
     5341 gaccgtaccg acgggccgaa tgttcgccgg gtgctggaag agagtctgcc tgcaaatttg
     5401 accttaatga tgaccgctac gggtgaatat cgtctggtgg attaacgttc ctgattagac
     5461 catcttccag tcgcaccaca cgagagaaat gacgccgggt aaatgcggcg tcatgactga
     5521 ttgctacgac gctcgtgcct cgctggccgc acttctccag ccagctttca aacacgctca
     5581 accaattttc gtcaaaatcc cgactcggct cgtccagcag caatagtggc ggagagagtg
     5641 cttccaggca ggcgacggcg accatccgtc gctgcgcact atgcagatcc aacgggtgcg
     5701 agttggcagt gtctgctaac tgacaacatt gccatgggta cgttgagtga tttcatccgc
     5761 cgggcatttt tgcagtttca aaccaaatgc gatctcgtca gcgacggtgc tgtgaaaaag
     5821 ttggttttcc gcttcctgaa acagtacgcc aacctttgcc gcgcgctggc ggtttttcag
     5881 gtttttcatc gcctgttgtt gcagcataac cgtaccggct gtgggcctaa gaagacctgc
     5941 cattactcgt aacagcgtcg atttccctgc gccattgtca ccggtcagcg ccaaccattc
     6001 accttgtttg agttgcagag aaatgtcgca aagacaatcc gttgccgcgc caggccagcg
     6061 ataagatatc ttatttaacg ttaacatagt ggctggagcg ctccgttttg caattgccac
     6121 gcttgctggc agtagttcag gaaaggtgtg cgatgacgtt caaaaagaat aatcagcgaa
     6181 ccacgttcaa acgcccagtg ttgtaagcgc tgcaacagca tttcgctggc ctgcggagtc
     6241 aggcggctaa aggcttcatc aagaattaag agctttggct gcatcgcgat ggcgcaggcg
     6301 atgaccacgc gctgtgtctc tccaccggag agtgttgccg ggtgacgatg gcgcaacggc
     6361 tggcactcgg tcagagtcag ggcggcatca atccgcgcca taatctctgc ttcagcaaga
     6421 cacagatttt ctggcccaaa ggcaacttct tcttccacgc taaaggtaca gccggagagc
     6481 tgcaaatagg gcgattgctg gacgagctgg atggtggcgg attgttcatt aagcgggaga
     6541 tgcccaatgg gcgtaccgag gagcgtcccc gtgcctgtta tctctcctgg cagaaagtcc
     6601 ggataccagc ccgccatcag ctgtgccagc gtacttttgc cactgccgtt gtcaccaaag
     6661 atggcgacca tccccggttt gacatagtga aagtcataac agaacggagg atgcgttgag
     6721 tgtgtcgggc aataacggaa ctgttctaac gtaaccatat ccacgctccg aattctgtca
     6781 gcatcagcaa gatcatggtg taacgcgcta cgcgttgtag ggtgctatcg gctggtgccc
     6841 acaacgttgt tcgtcggttg tggatccgaa atgcccgcat atccagcgcc gcaccacgga
     6901 tagccagatc gtttagcgca ttgtgtgtta gcggaataat taacgcgggc atcgcacgta
     6961 aacgctgata ccagccctca tccagcggca cgccacgggc acgctgcgct tcatgaataa
     7021 tcgctaattg ccgttttaac tgctcaacga ccaataacgg cccggcaaac agataagcga
     7081 cgcctggcgg caggcgagaa gcaaacagag cgcgaataaa acattgcacg gggacgtact
     7141 gcatccacaa ctgtgaggtt gaaacaatcg ccagaatccg caaccagagc gtaatagcat
     7201 gcgaccaccg ttctggcgag cgtggcgtgc cgcttagcca ttctggtagc cagccaccgt
     7261 gtaccagcca cagcccggct cccagcgaga acatcagcca gacgacatat tttgcccgcc
     7321 gtcgcgtggc ctttagcgca ataaggcaga aaaaggtcgc cgcgctatag atcggcaaaa
     7381 tggtctgcgc gggcaggata agcgtggtgc aggccgccaa tgcccacagt gttaatgagg
     7441 taaatggatg catttagcgc acggcagcca ttgccggaaa atggcgcgtg gtgcgaatcg
     7501 gcagctggcg cagtaaaagc cagacaatca ccgctgtaag aattttatcg accaggttcg
     7561 cgcctatcac cgtaatcgcc accgactcca ccaggttttg ccccatcgag tgcatccagg
     7621 caacgaacaa atcggctccg ctaccggtga caccgccgaa cagcgcagta cgcagcggta
     7681 cagcgacgac cgttaccgca agcgtaataa tcacgccgct gacaacgact tttggcaggg
     7741 tgcgaaacca acccgctcgc gccagccaac cggcgaccag gccgatgacc atcgcgacgg
     7801 gtgcaaatgc ggcggcgatc gggtcggtca gcaatcccca gagtaaattg gtcagtaagc
     7861 cggtcagcat tccgatcacc ggaccaagca gtaccgcgct aataagtgtg ccgatggagt
     7921 cgagaaatat aggtaatttg accatgctgg ccagttgccc gccgatcata ttgatggcga
     7981 tagatataac gatcaacacc agtgcctgac tggaaaagtg gctacgcgcc ataagaaaac
     8041 ctctttgaaa gtagttattt aacgggggaa atatgcaact cgtcgtaacc agaaagcgcg
     8101 caaggttggc ggctaaacgc cagttcctca aggctaccga tcaccagttc aagcgtggtc
     8161 cgcacggtac agtcgggcat catatgaccg ccaagcatcg tgccgtgtgg atcggaaacg
     8221 cagagatgaa gatgctcacc actctgttcc agcgtgccgt ttagtgagat cacttcgaat
     8281 gtaccgttca ggagcgtggt gccttcttgc ccggcatagc gcaaggcgac gtcagtcagg
     8341 ctaccggtac agcctgcaat ccaggcggca tgaagttgat gctgctgtgc aaaagcgtgt
     8401 agctgggaaa aaacttcctg cccggggagg aggcgcagtg cgtaaaagcg tgcggaggaa
     8461 gaatatgggt agggcaatgt catcattgga gtactccgaa actcggacgt attgcggata
     8521 atatcgacgt tgcatcagtc atattctgat ataagtcagg gtttgtgacg gcacaacgaa
     8581 aagattttgc tacgcttatt cgtgattctg caaagtggaa taaattccac aggggcgggg
     8641 ctgaaaacgt gacgtaagtc agataattgc tttctttgac tggacaatca ttccttttat
     8701 tccacgtttc gcttatccta gctgaagcgt ttcagtcgat taaatgttcg acaattaacc
     8761 aatcagtcgc agtttgcgac aggtaaggtt tccccggacg atttgctgga ttactctgtc
     8821 tggcaaattt gctcagggaa aaccttgcag gagatctatg accgtacgcg tagcgataaa
     8881 tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg tatgaatccg gacgccgggc
     8941 ggaaattacc gtggtggcaa tcaacgaact ggcggatgct gcgggcatgg cgcatttgtt
     9001 gaaatatgac accagccatg gccgttttgc atgggaagta cgacaggaac gcgatcaact
     9061 ttttgttggt gatgacgcca tccgcgtatt gcatgaacgt tcactgcaat cgctcccctg
     9121 gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc gtatatggct cccgcgagca
     9181 tggcgaagcg catattgccg ccggggccaa aaaagtgctc ttttcacatc ctggcagtaa
     9241 cgatctcgac gcgaccgttg tttacggcgt caatcaggat caacttcgtg cggaacaccg
     9301 catcgtttct aacgcttcct gtaccacgaa ttgcataatt cccgtcatca aattgttaga
     9361 tgatgcgtac ggtattgagt ccggcactgt gaccacaatt cactccgcca tgcacgatca
     9421 acaggttatt gatgcatacc atcctgacct gcgtcgcacc cgggcagcca gccagtcgat
     9481 cattccggtc gatactaaac tggccgccgg tatcacacga ttttttccgc aatttaacga
     9541 tcgctttgaa gcgattgcgg tacgtgtgcc aaccataaat gtgacggcaa tcgatttaag
     9601 cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac ctgttgctgc aaaaagcagc
     9661 acaaggtgca tttcatggta tagttgacta tacggaattg ccgttggtct ctgtagattt
     9721 taaccacgat ccgcacagtg ccattgtcga tggcacccaa acccgggtca gtggcgcaca
     9781 cctgatcaaa acgttggtct ggtgcgataa cgaatggggc tttgctaacc gaatgctcga
     9841 cacgacgtta gctatggcta ctgttgcttt caggtaagac gcaagcagcg tctgcaaaac
     9901 ttttagaatc aacgagagga ttcaccatgt ctgtaattaa gatgaccgat ctggatcttg
     9961 ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac gggaaagtaa
    10021 ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga actggccctg aaacaaggcg
    10081 caaaagtgat ggtaacttcc cacctgggtc gtcctaccga aggcgagtac aacgaagaat
    10141 tctctctgct gccggttgtt aactacctga aagacaaact gtctaacccg gttcgtctgg
    10201 ttaaagatta cctcgacggc gttgacgttg ctgaaggtga actggttgtt ctggaaaacg
    10261 ttcgcttcaa caaaggcgag aagaaagacg acgaaaccct gtccaaaaaa tacgctgcac
    10321 tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag gcttctactc
    10381 acggtatcgg taaattcgct gacgttgcgt gcgcaggccc gctgctggca gctgaactgg
    10441 acgcgctggg taaagcactg aaagaacctg ctcgcccgat ggtggctatc gttggtggtt
    10501 ctaaagtatc taccaaactg accgttctgg actccctgtc taaaatcgct gaccagctga
    10561 ttgttggtgg tggtatcgct aacaccttta tcgcggcaca aggccacgat gtgggtaaat
    10621 ccctgtacga agctgacctg gttgacgaag ctaaacgtct gctgaccacc tgcaacatcc
    10681 cggttccgtc tgatgttcgc gtagcaaccg agttctctga aactgcaccg gctaccctga
    10741 aatctgttaa cgatgtgaaa gctgacgagc agatcctgga tatcggtgat gcttccgctc
    10801 aggaactggc tgaaatcctg aagaatgcga aaaccattct gtggaacggt ccggttggcg
    10861 tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt ggctaacgct atcgcagaca
    10921 gcgaagcgtt ctccatcgct ggcggcggcg acactctggc agcaatcgac ctgttcggca
    10981 ttgctgacaa aatctcctac atctccactg gcggcggcgc attcctcgaa ttcgtggaag
    11041 gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa aaaatcacag
    11101 ggcagggaaa cctgcccttg tttcagcgcg cttttagagc acgcattttt tcatactcta
    11161 aataattcga gttgcaggaa ggcgacaaga gagtgaatcc ccaggagctt acataagtaa
    11221 gtgactgggg tgagcgaacg cagacgcagc acatgcaact tgaagtatga tgagtataag
    11281 gcccgacgat acaggacaag agacatgtct aagatttttg atttcgtaaa acctggcgta
    11341 atcactggtg atgacgtaca gaaagttttc caggtagcaa aagaaaacaa cttcgcactg
    11401 ccagcagtaa actgcgtcgg tactgactcc atcaacgccg tactggaaac cgctgctaaa
    11461 gttaaagcgc cggttatcgt tcagttctcc aacggtggtg cttcctttat cgctggtaaa
    11521 ggcgtgaaat ctgacgttcc gcagggtgct gctatcctgg gcgcgatctc tggtgcgcat
    11581 cacgttcacc agatggctga acattatggt gttccggtta tcctgcacac tgaccactgc
    11641 gcgaagaaac tgctgccgtg gatcgacggt ctgttggacg cgggtgaaaa acacttcgca
    11701 gctaccggta agccgctgtt ctcttctcac atgatcgacc tgtctgaaga atctctgcaa
    11761 gagaacatcg aaatctgctc taaatacctg gagcgtatgt ccaaaatcgg catgactctg
    11821 gaaatcgaac tgggttgcac cggtggtgaa gaagacggcg tggacaacag ccacatggac
    11881 gcttctgcac tgtacaccca gccggaagac gttgattacg catacaccga actgagcaaa
    11941 atcagcccgc gtttcaccat cgctgcgtcc ttcggtaacg tacacggtgt ttacaagccg
    12001 ggtaacgtgg ttctaactcc gaccatcctg cgtgattctc aggaatatgt ttctaagaaa
    12061 cacaacctgc cgcacaacag cctgaacttc gtattccacg gtggttccgg ttctactgct
    12121 caggaaatca aagactccgt aagctacggc gtagtgaaaa tgaacatcga taccgatacc
    12181 caatgggcaa cctgggaagg cgttctgaac tactacaaag cgaacgaagc ttatctgcag
    12241 ggtcagctgg gtaacccgaa aggcgaagat cagccgaaca agaaatacta cgatccgcgc
    12301 gtatggctgc gtgccggtca gacttcgatg atcgctcgtc tggaaaaagc attccaggaa
    12361 ctgaacgcga tcgacgttct gtaagatatt cctttctgct tatctcaagg cccgctctgc
    12421 gggtcttttt ttcgccaaaa gcaaaattgc ctgatgcgct acgcttatca ggcctacacg
    12481 tatcctgcaa tatattgaat ttgcaggttt ttgtaggccg gataaggcgt tcacgccgca
    12541 tccggcatga caaagcgcac attgatatct gtctgagccc cgctctgcgg ggattttttc
    12601 gccaaaagca aggtgattca gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta
    12661 ttgtttaccc ttgtcagact gcccgtcata aggcggcgga gtgtatttct ccattttgag
    12721 tcagttgaaa aggaatattg aatggaagat ttgaatgttg tcgatagcat aaacggcgcg
    12781 ggaagctggc tggtagctaa ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg
    12841 gcactcgcga tcatcatcgt tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat
    12901 cgcctgatga tctcccgtaa aatcgatgcc actgttgctg attttctttc tgcattagtc
    12961 cgttacggta ttatcgcctt tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca
    13021 gcgtcagtca ttgctgtact cggtgccgca ggcttagctg ttggtctggc tttgcagggg
    13081 tcactttcta acctggccgc tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga
    13141 gaatatgttg acctcggcgg cgtagccggt actgtgctga gtgtgcagat tttctccacc
    13201 accatgcgta ctgcagacgg taaaattatc gttattccga acggtaaaat tattgccgga
    13261 aatattatta acttctcccg cgagccagtt cgccgtaacg aatttattat tggcgtggcg
    13321 tatgattccg atatcgatca ggttaagcag atcctgacca atattatcca gtctgaagat
    13381 cgcattttga aagatcgcga aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt
    13441 aatttcgtgg tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg
    13501 ctggagcgta ttaaacgtga atttgatgcc gccggtatca gcttcccgta cccgcaaatg
    13561 gatgtgaact ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct gaggccagat
    13621 aatactctct ggcctctctc ttattagtta ttctgattgc caattaatat tatcaatttc
    13681 cgctaataac aatcccgcga tatagtctct gcatcagata cttaattcgg aatatccaac
    13741 gtgttttctt attactttca aggtcttgct cttggggcag ctatgattct gccgctcggc
    13801 ccacaaaatg cttttgtgat gaatcagggc attcgtcgtc agtaccacat tatgattgcc
    13861 ttactttgcg ctatcagcga tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg
    13921 ttattgatgc agtcgccgtg gttgctggcg ctggtcacct ggggcggcgt agtcttcttg
    13981 ctctggtatg gttttggcgc ttttaaaaca gcgatgagta gtaatattga gttagctagt
    14041 gccgaagtcc tgaagcaagg cagatggaaa attatcgcca ccatgttggc agtgacctgg
    14101 ctgaatccgc atgtttacct ggatactttt gttgtactgg gcagccttgg cgggcagctt
    14161 gacgtcgaac caaaacgttg gtttgcgctc gggacaatta gcgcctcttt cctgtggttc
    14221 tttggtctgg ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag
    14281 cgcattatca atctggtagt gggatgtgtc atgtggttta tagccttgca gctggcgaga
    14341 gacggtattg ctcatgcaca agccttgttc agttaggcgc tatctgatgg aaaaataaaa
    14401 cagaggcgct aagcttgcct ccagaggtcc tgagttttgg gcaagtcaat taacacggag
    14461 agactaacgt gaagttcaaa gttatcgccc tggcggcatt aatgggtatt agcgggatgg
    14521 cagcgcaggc taacgaattg ccggatggac cgcatattgt cacctccggt acggcaagtg
    14581 tggatgcggt gccagacatt gccactcttg cgattgaagt taacgtggcc gcgaaggatg
    14641 ccgctacagc caagaaacag gcagatgagc gcgtcgcaca atacatttcc ttccttgaac
    14701 tcaatcagat cgcgaaaaaa gatatcagct cagcgaattt acgcacccag ccagattatg
    14761 attatcagga tggtaaaagt atcctgaaag gctaccgcgc tgtgagaacg gtggaagtca
    14821 cgctccgtca gttagacaaa ctgaattcct tgctggatgg cgcgctgaag gcgggtctta
    14881 acgaaattcg ttctgtgtcg ctgggcgtgg cgcagcctga tgcctataaa gacaaagcgc
    14941 gtaaggcagc gattgataac gcgattcatc aggcgcagga actggcgaac ggctttcatc
    15001 gtaaactggg gccggtatat agcgtgcgct accatgtttc caactatcag cccagcccaa
    15061 tggtgcggat gatgaaagcc gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg
    15121 ccgctattca gtttgatgat caggtcgatg tggtcttcca gttagaacct gtggatcaac
    15181 aacccgctaa aacacctgca gcacaataat acttgtagcc cgacgtattc gtcgggctaa
    15241 tgcctttacc cgatattgcc tcctttttct gtaattttct gtaaaaccat gacgtaatca
    15301 cctatctttg ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg
    15361 atgcggggag gggggatgga catttttatt tcgaaaaaga tgcgcaattt cattttatta
    15421 gcgcaaacca ataatattgc ccgggcggca gaaaaaatcc atatgtcggc ttcgccattt
    15481 ggtaaaagca ttgccgcgct ggaagaacaa attggctata cgctatttac ccgcaaagat
    15541 aataatatca gcctcaataa agccggtcag gaactgtatc aaaaactgtt cccggtttat
    15601 caacgacttt ctgcgattga taatgaaatc cataactcag ggcgtcgttc acgagaaatt
    15661 gtgattggca tagataatac ttatccgaca attattttcg atcagttgat tagtctcgga
    15721 gataagtatg aaggcgtgac cgcccagcca gttgagttca gcgaaaacgg cgttatcgat
    15781 aatctttttg accgtcagtt agattttatt atctccccac agcatgtatc tgcacgagtt
    15841 caggaactgg aaaacctgac tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt
    15901 tctcgccgtt atgaagagcg tcaagagcaa gagctgcttc aggagctgcc ctggctacaa
    15961 atgcgctttc aaaaccgggc taacttcgaa gcaatgattg atgcgaatat gcgaccttgt
    16021 gggatcaatc ccaccattat ttatcgtccc tacagtttta tggccaaaat cagcgccgtg
    16081 gagcgcggac attttctgac ggtgatcccg cattttgcct ggcgactggt gaatccggca
    16141 acgctgaaat attttgatgc gccgcacagg ccgatgtata tgcaggaata tctttattca
    16201 atcagaaatc atcgttatac cgccacgatg cttcagcata ttgctgaaga tcgtgacggg
    16261 acaaaccatt aacccagcat cgagccggtt gcaattaaat tacggtgtaa gtcgaagacg
    16321 tggctaagat cgtggtgaat atgtccgccg ggcgcatttt ccagatagcg atgcagataa
    16381 tcccgataca taggatgtgc acaattatca atgatagtgc gggcgcgttg aagcggggaa
    16441 agaccgcgca gatcggcgat cccttgttcg gtgatgatca ctttgacgct gtgttcgctg
    16501 tgatcaacat ggctgcacat tggcacgacg gttgagatct tgccttcttt agcaatcgac
    16561 ggagccataa agatcgacag atacgcgttg cgttcaaaat caccgctgcc gccgatgccg
    16621 ttcatcagat cgaccccggc tacgtgcgtt gagttggcat gcccgtaaat atcaaactcc
    16681 aggccgacgt tcagagcgat gacgcccaga cgacggatga tttccgggtt attggaaatc
    16741 tcctgcggac gcaacacaat gcggctggca aagtaatcca tattgtcgta aatcttgcgc
    16801 agggaatcgg ccgagattgt caggctggag gcgctggccc cgctgatttt gccggtttcc
    16861 agtaaatgca ccaccgattc ctgtagcact tccgaataca tcataaacgg aggaatttcc
    16921 gggttttccc ccagacgcgc cattaccgca ttattgatat tgcccacgcc actttgcagc
    16981 ggcagaaatt ccggcggaat acgcccatgc gccatttcct gcaataagaa cgtgaccacg
    17041 ttatcggcaa tctgctggca catgggattt tgcttatcca gcatattacc ggcgtcgggc
    17101 aagttggttt ccacgacggc gacaatcttt ttcggatcga tttgcacata gcgggtaccg
    17161 acgcgatcca ttgcatggaa gatcgacacg ctattgcgcc gcggtggcgc gccaggaatc
    17221 acaatatccg ccagttctgc aacgcgcgga tcgtgatagt gattaagttc aatgatcact
    17281 ttcttcgccc gcagcagcca tgtcggcgca ttaccgatcc cgctggttaa ccagactcga
    17341 ccatccggtg ccagtgccga tgcttcgatg acggcaacat caatgtcgcc gaagaaaccg
    17401 taattgacca tttgcgccac ttcgctcaaa tgcaggtcaa cgaaactcac cgcgccctga
    17461 ttgatctttt tacgtaaacc ggacgatgtt tgatatggcg cacgccagga aacagcatcg
    17521 gcgtcagaaa gtacatcgtc agcggcggcg ctgattgacg cccccgtcag caggcgaatt
    17581 tgatacggct ttttggcctc atgctgttcg ttagctctgc gggcaatcgc ggtcggtaag
    17641 gctttcggcg aacccgctgg ggtaaagccg ctaaatgcca ccatgtcgtt atgctggata
    17701 atttctgccg cttcatcggc ggtcatcctt gtccactgag tttccatttt tactccttcg
    17761 ttcgcaggga ttaatgacca acgaaattag gtttacgttt ttcgaggaaa gcgttcatcc
    17821 cttcctggta atcttcgctg tcataaaccg cgcggcgcat cccctgaata cgttcaaatt
    17881 catcggagtt catggtgtgc gcttcgccca gtacacgcag ctcttctttg ataacagcaa
    17941 tggctaacgg cgctttctca gagatgtggt gcgccatttg taaggtgaaa tcttccagtt
    18001 cttccacttc cacaacatgg ttgaggatgc cgacagccaa cgcgcgctgg gcggtgattg
    18061 gcgaagcggt aaaaatcagt tctttgacaa tgtggaagcc cgcgtcgcgg gtcaggttgt
    18121 gaatgccgac caggttatac gggacgccga ggtttacagg cgtcattgag aaggttgagg
    18181 tactggcggc gataatcaga tcggaactca tgatcatttc aaatgcgcca ccccaaacac
    18241 taccttccac catcgaaatg atcggtttcg ggaatttttg gatcatgcgg gtgatttgac
    18301 gcaatggatc atcataggag agcggatcgc gaccgccaga cggcagttcg tgaatagcgt
    18361 ggcctgcgga gaagactttg gatccactcg gcgcgcgcaa aatgatacag cgaatttccg
    18421 gccggttgag atcgcttaat gcctgcataa gatcatcaat aaagacttta cttaaggcat
    18481 taagtttgcg gccatagtta aactcaatga ccgccacttt gttgatagtg acaacgttaa
    18541 catactgata agacataaaa attcctttaa tcaaaatatt gcgtctggat aaattcactg
    18601 agctgccgca ggccggtgcg cggtgagagc gtattgtttt tgaccgctaa aagcgtctgg
    18661 cggtaatagc gatcgaaatc ttcattcgcg aacaggtgat tcagtacttc ttcttcggtc
    18721 tgcttacgca gccattccac cgattgttgt tgccgcactt gttgtaaacg accactggca
    18781 gttagcgcgg ttttgaagtc gatgatggcg tgccagatct catcgattcc acgtttttcc
    18841 agtgcgctac aagtcagaac ccgtggctgc cattcgtcgt atttacgtcg cagaatatgc
    18901 agggcactct cgtacatatg ccgggcaatg gcgacattgg tatggttatc gccatcgtct
    18961 ttgttgataa cgatcagatc agccacttcc atcagccctt ttttaatgcc ctgcagatca
    19021 tcgccgccac cggcaatttg caacgagata aaacagtcca ccatgcgggc gacttctgtt
    19081 tccgactgcc cgacgccaac cgtttcgaca atcactacgt cataacccgc tgcttcgcat
    19141 aacagcatta attcccgcgc tcgctgactg gcaccgccca gatgaccgga ggatggtacc
    19201 gggcgaataa acgccgcttc ggcacgcgcc aggtcattca tgcgggtttt atccccgaga
    19261 atgctaccgc cagtgaccgg gctgctggga tcgaccgcaa taaccgcgac ctttaatccc
    19321 tctcgaatca acaacatgcc aaaggcctca agaaaggtac ttttccccgc gccgggggtg
    19381 ccggtaacgc ccagtcgcag ggtgttaccg cagtacggca taatggcatc aagcagctgc
    19441 gtacttagtg cctgatgacg cgggtgacgg ctttccacca gcgtcatggc ctgggcgagt
    19501 gtggcacgct caccctgacg taagcggcga atactttctg ccagcgtggc ttcattaatc
    19561 atgatgctgg cttatcagat tcagtacgtc gcgcacactg tcgagcatag gtgtacctgg
    19621 accataaatc gccgccacgc cgcgctcttg caggaaggcg taatcctgcg gcggaatgac
    19681 gccacccgcg accacgcaga tatcttcgcg tccccatttt ttcagcgctt cgaccagttc
    19741 cgggatcagc gttttatgac cggcagccag tgaggatgcg cccactacgt gaacgtcgtt
    19801 ttctacggcc aggcgggcga tctcttcagg tgtagagaac atcgggctta aatctacgtc
    19861 gaaaccgaga tcggaatagg cgctggcgat cactttcgcg ccgcgatcgt gtccatcctg
    19921 gcccatctta gcgatcagaa tgcgcgggcg acgaccattg tcggcaagga actgctccgt
    19981 ttgcgcaaca atggcatcga actcggaggc cgatttctca gactgatgat agctttgcgc
    20041 aatcacgccg gtaacacact ggcttggcac cagataacgg tcgaaagcga cttccagcgc
    20101 atcggaaatt tcacccaggg tggcgcgaac gcgagcggca ttaacagcgg cagccagcag
    20161 gttttcgtta tgctgtgcgg cgtgagtcag ggcgttcaac gcggcggtta cggcggcatc
    20221 atcacgggtg gcgcgaatgc gttccagcga agcaatttgc tcgttacgca ccatcacgtt
    20281 gtcgatctca agtacatcgg tttcgtcttc gtgatccagt ttgtacttgt tgacaccaac
    20341 gatgacacgc ttgccctggt cgatcagcga ctgttcgcgc gctgaggcct cttcgatcat
    20401 tcgttttggc agacctgctt cgatcgcttt cgccatgcca ccggcttcgt cgatctgttg
    20461 gataatagct ctggcttgtt tgacgatttg atcggtcagc gactcaatgt aataggatcc
    20521 ggccagtgga tcgacgatgc ggcagagttc tgattcttcc tggatgatga tctgggtgtt
    20581 gcgggcaatg cgtgctgaga aatcggtagg caaaccaagc gcttcgtcaa aggcgttggt
    20641 atgcagtgac tgagtaccgc ccagcgtcgc agccagcgct tcaatggtgg tgcggataac
    20701 gttgttatac ggatcctgtt cagtcaggct ccagcctgag gtctggcagt gggtacgcag
    20761 cgccagtgat ttcgggtcct gtgcgccaaa tccactgacc gcttcgctcc ataaataacg
    20821 tgccgcacgc aacatggcga cgttcataaa cagatccatg ccgatgccga agaagaacga
    20881 caggcgagga gcgaagtcat caattttcag tccggcagag attgctgctt tgatgtactc
    20941 aatcccatca gcgagcgtaa atgctacctg ctgcacgcag ttggcacccg cttcacccat
    21001 gtggtaaccg ctgatactga tggtattaaa tcgcggcatg ttgccggaac accaggcgat
    21061 gatgtcggcg ataatgcgca ttgacggttt tggcgggtaa atataggtgt tgcggcagag
    21121 gtactctttg aggatatcgt tctgaatggt gccggtcagt ttatcaggtg taacaccttg
    21181 ctcttccgcg gcgatgatat aaaacgccag tactggtagc actgcgccat tcatggtcat
    21241 cgaaaccgac attttatcca gcgggatctg gtcgaacagg actttcatat cttccacggt
    21301 gtcgatagcg acgcccgctt tgccgacgtc acccgccacg cgtgggttat cggagtcgta
    21361 gccacggtgg gtggcaaggt caaacgcaac ggaaagacct ttttgcccgg cggccaggtt
    21421 acggcgataa aaagcgttgg actcttttgc tgttgaaaaa ccagcatact gacggatggt
    21481 ccacggttgg gcggtataca tagtggcacg cgggccacga acgtagggcg gcaaaccagg
    21541 aagggtacct gtcacctcca gattatcgag atcggcttcg gtatacagcg gcttgatggc
    21601 gatcccttcc gcggtttgct gaaccagcga gtcgacagtt ttctcccgac ggctcaattc
    21661 cttgttggca agctgttgcc actcctgctc gttagacatt atttgctccg taaaacgcaa
    21721 tcaaaatgag ggtatttgcg cttacggtga gctttttacg tgggtttgtc gccagcaaaa
    21781 aaaatgcatt cagacgtcgt tttttgccgc ctgagatttg tctattttgt gatgtgattc
    21841 aaggaataca aaaagaagtg aggataacgc ctgatatgtg ctatcaggca ttatttgatg
    21901 gattaatcct gacgaaggac tttgtgacca taatcgagca acgcatcagt gactttacgc
    21961 atcatgcggc tttcaggagc aaagcggtgc cagtagagca tccggcgttg aaatagccct
    22021 ggcgttaagt caatcagttc accgctggct agttctttct cgatttgcag gtgcgggatc
    22081 atacagcagg tggtgccctg gcgagcaagt tgcacgaacg cttctgaaga attaacgata
    22141 tggcagggca cgctgcctgg aggcagatcg aagttttgct gcaaaaaggc ctggtgcata
    22201 tcgtcaagat ggtcaaaggc gaccacaggt gctttcaata atgccgaacg cgttacgccg
    22261 ttagggaaat atttttcggc aaagggtttt gagctgacga acagatagtc gagcgcacca
    22321 agtttatcga caagacaact cggcagcgcc tgatgttgaa tactcaccgc gccgaccact
    22381 tcgccgcggc gcagacgttc ctgagtgcgg gtttcatctt ctacctgcaa gttgaggcgg
    22441 ataggcgaat cagccaacac aggagccagt gcaggaagca accacgtcgc cagactgtcg
    22501 gcgttgaccg ccagtgaaag cagcagcgga gtcgaaccgg tttgttcatc gcccagccac
    22561 tcttcttcca gcaactccac ctggcgcagc agtgccagca gtttttgccc ttgttccgtc
    22621 gggcgcggcg gtacggtacg caccaacagc ggctgcccga acatattttc cagttgctta
    22681 atgcgctgtg agacggctga ttgtgtaatg cacagctttt gtgccgcgcg ctcaaatcct
    22741 cgttcacgta tcaccgcatc cagtgcctgt aatgttctgt agtccgggcg tttcattgct
    22801 gttgtctgct cctggtaaat gctttttcct gcactatgac acaattttgt gtcaggttgc
    22861 atataccgaa gccgtcggcg ggtaacattt gtattcgtgg ttaaacgcgc agcttaaaag
    22921 atcagggatt tgcgctaatg catttcgctc agcgagttcg cgccctggtt gtgttgaatg
    22981 gcgtggcgtt attgcctctg ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt
    23041 tatttgcggg gcgaaagggg atgcccgcca ttgcgcgata ttttatggat gagttaacca
    23101 cgcggcttgc caacggggtc tgaatcgctt tttttgtata taatgcgtgt gaaatttcat
    23161 accacaggcg aaacgatcat gacgcaggat gaattgaaaa aagcagtagg atgggcggca
    23221 cttcagtatg ttcagcccgg caccattgtt ggtgtaggta caggttccac cgccgcacac
    23281 tttattgacg cgctcggtac aatgaaaggc cagattgaag gggccgtttc cagttcagat
    23341 gcttccactg aaaaactgaa aagcctcggc attcacgttt ttgatctcaa cgaagtcgac
    23401 agccttggca tctacgttga tggcgcagat gaaatcaacg gccacatgca aatgatcaaa
    23461 ggcggcggcg cggcgctgac ccgtgaaaaa atcattgctt cggttgcaga aaaatttatc
    23521 tgtattgcag acgcttccaa gcaggttgat attctgggta aattcccgct gccagtagaa
    23581 gttatcccga tggcacgtag tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg
    23641 gaataccgtc agggcgtggt gaccgataat ggcaacgtga tcctcgacgt ccacggcatg
    23701 gaaatccttg acccgatagc gatggaaaac gccataaatg cgattcctgg cgtggtgact
    23761 gttggcttgt ttgctaaccg tggcgcggac gttgcgctga ttggcacacc tgacggtgtc
    23821 aaaaccattg tgaaatgatc tgacggggga acctcccccg ttaaaaaaat tctcttcatt
    23881 aaatttggtg acatgtgtca cgtttttacc tggctattgt cgattgctct aaataaatct
    23941 tctaaaccag catattcatc caagaattac ctttgcgtga tatttcctca acatcgcgac
    24001 gcaaacgttc atattgccgc aatattattt tttgatatgt tgaaaggcgg acgcaaatcc
    24061 gcacacaaca tttcaaaaga caggattggg taaatggcaa aggtatcgct ggagaaagac
    24121 aagattaagt ttctgctggt agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca
    24181 gctggttaca ccaacatcga atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa
    24241 tccatccgcg atgcccactt catcggcctg cgatcccgta cccatctgac tgaagacgtg
    24301 atcaacgccg cagaaaaact ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt
    24361 gatctggatg cggcggcaaa gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg
    24421 cgctctgttg cggagctggt gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa
    24481 gccaatgcta aagcgcaccg tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg
    24541 cgcggcaaaa agctgggtat catcggctac ggtcatattg gtacgcaatt gggcattctg
    24601 gctgaatcgc tgggaatgta tgtttacttt tatgatattg aaaataaact gccgctgggc
    24661 aacgccactc aggtacagca tctttctgac ctgctgaata tgagcgatgt ggtgagtctg
    24721 catgtaccag agaatccgtc caccaaaaat atgatgggcg cgaaagaaat ttcactaatg
    24781 aagcccggct cgctgctgat taatgcttcg cgcggtactg tggtggatat tccggcgctg
    24841 tgtgatgcgc tggcgagcaa acatctggcg ggggcggcaa tcgacgtatt cccgacggaa
    24901 cctgcgacca atagcgatcc atttacctct ccgctgtgtg aattcgacaa cgtccttctg
    24961 acgccacaca ttggcggttc gactcaggaa gcgcaggaga atatcggcct ggaagttgcg
    25021 ggtaaattga tcaagtattc tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa
    25081 gtctcgctgc cactgcatgg tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc
    25141 gtgctaactg cgctgaacaa aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat
    25201 ttacaaactt ccgcccagat gggttatgta gttattgata ttgaagctga cgaagacgtt
    25261 gccgaaaaag cgctgcaggc aatgaaagct attccgggta ccattcgcgc ccgtctgctg
    25321 tactaattcc ccttctctga aaattaacgg gcaggtcact gacttgcccg tttttttatc
    25381 ccttctccac accgttgccg cgttgcgcat tctactttgc gagtctgctc cagaaaataa
    25441 atcaaagtca gatcaatgcg ttgtgtttat ttaatgggga tttacgatgg cgggacagca
    25501 tggggctgta acggtaaagc cctcaccgaa gcgagggctt aaaggagaag ggttatgatg
    25561 cgacttgtca tcatactgat tgtactgtta ctgataagtt tcggagctta ttaacagtca
    25621 gtctcagggg aggagtcatc ctcccttacc cttactcact aaattaggtc aaagaatcaa
    25681 cgatgtcaat cagggcgatg cggggtgcat cgccctgaac cgaacttttt gatagccact
    25741 tcatcgccca cgtttactgc cgtaatagat cctttttccc aaaaattatc tctgttttgc
    25801 atggggtttt tcgagcacgc tcgaagaaaa gatttaaagg tagatcaatg cgttgtggtt
    25861 atttaaagag agcttacgat agctgggcag catggggctg taacggtaaa gccctcaccg
    25921 aagcgagggc ttgaaggaga agggttatga tgcgacttgc catcatactg attgtactgt
    25981 tactcataag tttcccagct tattaacagt cagtcacagg ggaggagcaa tcctccctta
    26041 cccttactca ctaaattagg ttaaagaatc aacgatgtca atcagggcga tgcaggtgta
    26101 tcgcccttac cactcccaga ctttcgacgg tgtaaccacc gcaggaagag ggatatccca
    26161 ctcttcaacg gggagttttt ccaccaactg acaatcatgc gcataaccca ccggttgcgt
    26221 tttatagtgc tgccagtttt gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg
    26281 ctgaccgtac tcatcaaagg cgaccagcgg tgtgatcagc acgtctaatc gggaaagggg
    26341 tagcacgtca cgcacatcca gttttggctc atggatcttc aacctgttca tcaccagttc
    26401 gctttgcgga tggtaattca ggaacagcaa attaccggca ctaaagggat gcaaaactgg
    26461 aagatatacg cgcttaccgg cgcgccagag ttgttctatt agcggctggg tgtcgagttc
    26521 accatcaaaa gagaggaata cagcgaccgt atgcgccatc accaccgggg gataagtcat
    26581 catccgggta gcggcttgtt gacccatttc ctgctgttgt tccggcgtta acgcacgacg
    26641 acgttgccga atcattttgc ggatttcttg tcgggataat gtcagtggga gttctggtag
    26701 ttgcgtcatg gctggtgcca gataagaagg gaatctccga gatgccgccg caggctgtaa
    26761 cccttgaacc cttggttcaa ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgga
    26821 ccgagcatgc tcaccaaccg cggagcgcca cattcttgtg gtatgaaata tcggctcagg
    26881 ggactggccc gcttgcgaac atctcagaga aattttgtct tcacggttac tctaccacag
    26941 taaaccgaaa agtgtcattc aaagttttgg ttagtttttt cggtgatgcg accttgttca
    27001 agtaacgctt gttctatggt ctgctgcagc atccgaatac gctgttccat acttgccgcg
    27061 tagtcacgag tcttcgcttt ttcttgcgct aactcatagc tgatattcaa tgcggcaatg
    27121 aagaccaact gttcagtatt tgtgactcta gtgcgttctt tcagatcttg caaccgttgg
    27181 ttcagatcgt ccgctgcctg attcaacgca tccctttggt caggcgggca gttcacacgc
    27241 agtgaacggc caaaaatttg gatatcgacg ggttgtgcag acatgccacc ttcctgctga
    27301 ttgactgcgc tgccttcgtc tttagaccca tggtctgcga aggggcgaca ctatagctac
    27361 cctgatgaga agagacaagc ccttttctgg tccaccaggg cccaaagtgg tagcatatca
    27421 tgaatattcc tccctttgac gacgaatgct tatgtctata cagaacgaaa tgcctggtta
    27481 caacgaaatg aaccagtatc tgaaccaaca agggacgggt ctgacccctg ctgagatgca
    27541 tggtttaatc agtgggatga tatgtggcgg taacgatgac agctcatggc taccgctact
    27601 tcacgacctg acgaacgaag gcatggcttt cggtcatgag ctggcacagg cactgcgtaa
    27661 aatgcactct gccaccagcg atgccctgca ggatgacggc ttcctttttc agctttatct
    27721 gcctgatggc gatgatgtca gcgttttcga tcgggctgat gcattggcag gttgggtcaa
    27781 tcacttcctg cttggtcttg gcgttacgca accgaagctg gataaagtga ccggcgaaac
    27841 cggtgaagct atcgacgatc tgcgtaacat tgcgcaactg ggttacgacg aagacgaaga
    27901 tcaggaagag cttgaaatgt cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct
    27961 gttatgccac gacaccttta ctcatccgca accgaccgcg ccagaagtac aaaaaccgac
    28021 tctacactaa aaacaaaaaa cgtaaggaga gtgttatgag tgagatatcc cggcaagagt
    28081 ttcagcgtcg ccgtcaggcc ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt
    28141 ttgctgcacc agaagtaaca cgtagcgccg acagcgaata cccctatcgt cagaacagtg
    28201 acttctggta cttcaccggc tttaacgaac cggaagcggt gctggtgctg attaaaagcg
    28261 atgacactca taaccacagc gttctgttta accgcgttcg cgacctgacg gcggagatct
    28321 ggtttggccg tcgcttaggc caggatgccg cgccagagaa actgggcgtt gaccgcgcac
    28381 tggcattcag cgaaatcaat cagcaacttt atcaactact taacggcctg gatgtggttt
    28441 accatgccca gggcgaatat acctatgctg atgaaatcgt gaacagtgcg ctggaaaaac
    28501 tgcgtaaagg ttcgcggcaa aatctcaccg caccggcaac gatgatcgac tggcgtcctg
    28561 ttgttcatga aatgcgcctg ttcaaatcgc cagaagagat tgccgtactc cgccgcgcgg
    28621 gagaaatcac cgccatggca catacacggg cgatggaaaa atgccgtccg ggaatgttcg
    28681 agtaccatct ggaaggcgaa attcaccacg aatttaaccg ccacggtgcg cgctatccgt
    28741 cctataacac cattgtcggc agcggtgaaa acggctgcat tctgcactac accgaaaacg
    28801 agagtgaact gcgcgacggt gacctggtgt tgattgacgc gggttgtgaa tacaaaggtt
    28861 acgcgggcga tattacccgc accttcccgg tcaacggcaa attcacccag gcccagcgtg
    28921 aaatctacga cattgtgctg gagtctctcg aaaccagcct gcgcctgtat cgtccgggaa
    28981 cttccattct ggaagtcact ggtgaagtgg tgcgcatcat ggttagcggc ctggtaaaac
    29041 tcggcatcct gaaaggtgat gttgatgaac tgatcgctca gaacgcccat cgtcctttct
    29101 ttatgcatgg ccttagccac tggttaggac tggatgtcca tgacgttggc gtttatggtc
    29161 aggatcgctc gcgcattctg gaaccgggga tggtactgac cgtagagcct ggtctgtata
    29221 tcgcgccgga tgcggatgtg ccagaacaat atcgcggtat cggcattcga attgaagacg
    29281 acattgtgat taccgaaacc ggtaacgaaa acctcaccgc cagcgtggtg aaaaagccgg
    29341 aagaaatcga agcgttgatg gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg
    29401 cggcatggcg ggcgcgacgc tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc
    29461 ggtacatttg attgaagcga ctgcgccaga gtcacatgct catccgggct ttgatggacg
    29521 agcgatagcg ctggcggcgg gtacctgtca gcaactggcg cgcatcggcg tctggcaatc
    29581 tctggcggat tgcgcaactg ccatcaccac cgtgcatgtc agcgatcgtg gtcacgctgg
    29641 atttgtcacc ctcgccgcag aagattacca actggcggcg ctgggacagg ttgtcgaatt
    29701 gcacaatgtc gggcaacggc tgtttgcatt gctgcgtaaa gcacctggcg taatgctgca
    29761 ttgccccgat cgcgtagcta acgttgcccg tactcagagt cacgttgaag tgacgctgga
    29821 gagtggcgag acgctgacgg gccgcgtgct gatagccgct gatggcaccc attcagcgtt
    29881 agccaccgcg tgcggcgttg actggcagca ggagccttac gaacaactgg ccgtgattgc
    29941 caacgttgcg acttccgttg cgcacgaagg ccgcgctttt gaacgcttca cgcaacatgg
    30001 cccgctggcg atgttgccga tgtctgacgg acgctgttcg ctggtctggt gtcatccact
    30061 ggaacggcgc gaagaggtgc tgtcgtggag cgacgagaag ttttgccgtg aactccagtc
    30121 ggcctttggc tggcgacttg gaaaaattac ccacgccggt aaacgcagtg cttatccgct
    30181 ggcgttaacc cgcgccgcca gagctattac ccatcgtacc gtgctggtgg gcaatgcggc
    30241 gcaaactctg cacccgattg ccgggcaagg gtttaacctc ggtatgcgag atgtcatgag
    30301 ccttgcggaa accctgactc aggcgcatga gcgcggagaa gacatcggtg attacggcat
    30361 attgtgccgc tatcagcagc gtcgacagag cgatcgcgaa gcaaccatcg gcgtcacgga
    30421 cagcctggta catctttttg ccaaccgttg ggcaccgctg gttgtcgggc gcaacatcgg
    30481 actgatgacg atggaattat tcaccccggc acgcgatgtg ctggcgcagc gcaccctcgg
    30541 ttgggtggcg cgttgaaggc ggttttaagg aataaacaat gcaaagtgtt gatgtagcca
    30601 ttgttggtgg cggcatggtg gggctggcgg ttgcctgcgg cttacagggg agcggcttac
    30661 gcgttgccgt actggagcag cgcgtaccgg aacctctggt ggcggatgca ccaccacaac
    30721 tgcgcgtttc ggctatcaat gccgccagcg aaaaattact cacccgtctt ggcgtctggc
    30781 aggacattct ctctcgtagg gccagttgtt atcacggtat ggaagtgtgg gacaaagaca
    30841 gttttggtca catttcgttt gacgatcaaa gcatgggcta tagccatctt gggcatatcg
    30901 ttgaaaactc agtgattcac tacgcgctgt ggaacaaagc gcagcagtcg tcagatatca
    30961 ctctgttggc ccccgcagaa ttacagcagg tcgcctgggg agaaaatgaa actttcctga
    31021 cgctgaaaga tggcagcatg ttaacggcgc gtctggtgat tggcgcggac ggtgctaatt
    31081 cctggttgcg caacaaagca gatattccgc tgactttctg ggattatcag catcacgcgc
    31141 tggtagcgac cattcgcacg gaagaaccgc atgatgcggt ggcgcggcag gttttccatg
    31201 gcgaaggcat tctggccttt ttaccgctta gcgatccgca tctttgctca attgtctggt
    31261 cactgtcgcc agaggaagcg cagcggatgc agcaggcaag tgaagatgaa tttaatcgcg
    31321 cgttaaatat cgcttttgat aatcgcctgg gcttatgcaa ggttgagagc gcgcgtcagg
    31381 tgttcccact gacggggcgt tatgcgcgcc agtttgccgc gcaccgtctg gtgctggtgg
    31441 gcgacgccgc gcataccatt cacccgctgg cggggcaggg agtaaatctc ggctttatgg
    31501 atgctgcaga gctgattgct gaactgaaac ggttgcatcg tcaggggaaa gacatcgggc
    31561 agtacattta cctgcgccgg tatgagcgta gccgcaagca cagtgcggcg ctgatgctgg
    31621 ctggtatgca gggattccgc gatctgtttt ccggtaccaa tccggcgaaa aaactgctgc
    31681 gtgatattgg tttgaaactg gccgacacgc ttcctggcgt taaaccacaa cttatccgcc
    31741 aggcaatggg attaaacgat ttgcctgaat ggctgcgtta aaaatttctc ttctgctgtt
    31801 tatttgatac ccatcacact ttcatctccc ggttttttcg ccgggagatt ttcctcattt
    31861 gaaataaact aatttcacct ccgttttcgc attatatttt ctaatgtcat tattttttga
    31921 tttagtgttt tttgacattt ttttagctct taatattgcc ttattcaaat tagctttcta
    31981 atggcatcat ctttatatag aaactggtgt gttttttggt tttttattct gtcgcgattt
    32041 tggcgttttt taaccataag ctaatgtgat gatcaatttt accttatggt taacagtctg
    32101 tttcggtggt aagttcaggc aaaagagaac gattgcgttg gggaccggga gtggctccga
    32161 tgctgggttt cgtggtgata atttcaccat gaaaagttgt cagccccgct tattcaatga
    32221 ggacaagatg gcacaacaga ctcctttgta cgaacaacac acgctttgcg gcgctcgcat
    32281 ggtggatttc cacggctgga tgatgccgct acattacggt tcgcaaatcg acgaacatca
    32341 tgcggtacgt accgatgccg gaatgtttga tgtgtcacat atgaccatcg tcgatctccg
    32401 cggcagccgc acccgggagt ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac
    32461 caaaagcggc aaagcccttt actcggggat gttgaatgcc tctggcggtg tgatagatga
    32521 cctcatcgtc tactacttta ctgaagattt cttccgcctc gttgttaact ccgccacccg
    32581 cgaaaaagac ctctcctgga ttacccaaca cgctgaacct ttcggcatcg aaattaccgt
    32641 tcgtgatgac ctttccatga ttgccgtgca agggccgaat gcgcaggcaa aagctgccac
    32701 actgtttaat gacgcccagc gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca
    32761 ggcgggcgat ctgtttattg ccaccactgg ttataccggt gaagcgggct atgaaattgc
    32821 gctgcccaat gaaaaagcgg ccgatttctg gcgtgcgctg gtggaagcgg gtgttaagcc
    32881 atgtggcttg ggcgcgcgtg acacgctgcg tctggaagcg ggcatgaatc tttatggtca
    32941 ggagatggac gaaactattt ctcctttagc cgccaacatg ggctggacta tcgcctggga
    33001 accggcagat cgtgacttta tcggtcgtga agccctggaa gtgcagcgtg agcatggtac
    33061 agaaaaactg gttggtctgg tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt
    33121 acgttttacc gatgcgcagg gcaaccagca tgaaggcatt atcaccagcg ggactttctc
    33181 cccgacgctg ggttacagca ttgcgctggc acgcgtgccg gaaggtattg gcgaaacggc
    33241 gattgtgcaa attcgcaacc gtgaaatgcc ggttaaagtg acgaaacctg tttttgtgcg
    33301 taacggtaaa gccgtcgcgt gatttacttt tttggagatt gattgatgag caacgtacca
    33361 gcagaactga aatacagcaa agaacacgaa tggctgcgta aagaagccga cggcactttc
    33421 accgttggta taaccgaaca tgcccaggag ctgttaggcg atatggtgtt tgttgacctg
    33481 ccggaagtgg gcgcaacggt tagcgcgggc gatgactgcg cggttgccga atcagtaaaa
    33541 gcggcgtcag acatttatgc gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg
    33601 agcgattccc cggaactggt gaacagcgaa ccgtatgcag gtggctggat ctttaaaatc
    33661 aaagccagcg atgaaagcga actggaatca ctgctggatg cgaccgcata cgaagcattg
    33721 ttagaagacg agtaacggct ttattcctct tctgcgggag aggatcaggg tgaggaaaat
    33781 ttatgcctca ccctcactct cttcgtaagg agagaggttc acaattcact gcacgtttca
    33841 ggaaccatcg ctcatgacac agacgttaag ccagcttgaa aacagcggcg cttttattga
    33901 acgccatatc ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg ttggtgcaca
    33961 atcgttaaac gcgctgaccg gccagattgt gccgaaagat attcagcttg cgaccccgcc
    34021 gcaggttggc gcaccggcga ccgaatacgc cgcactggca gaactcaagg ctattgccag
    34081 tcgcaataaa cgcttcacgt cttacatcgg catgggttac accgccgtgc agctaccgcc
    34141 ggttatcctg cgtaacatgc tggaaaatcc gggctggtat accgcgtaca ctccgtatca
    34201 acctgaagtc tcccagggcc gccttgaagc actgctcaac ttccagcagg taacgctgga
    34261 tttgactgga ctggatatgg cctctgcttc tcttctggac gaggccaccg ctgccgccga
    34321 agcaatggcg atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc
    34381 ttccgatgtg catccgcaaa cgctggatgt ggtccgtact cgtgccgaaa cctttggttt
    34441 tgaagtgatt gtcgatgacg cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct
    34501 gttacagcag gttggcacaa ccggtgaaat ccacgactac accgcgctta ttagcgaact
    34561 gaaatcacgc aaaattgtgg tcagtgtcgc cgccgatatt atggcgctgg tgctgttaac
    34621 tgcgccgggt aaacagggcg cggatattgt ttttggttcg gcgcaacgct tcggcgtgcc
    34681 gatgggctac ggtggcccac acgcggcctt ctttgcggcg aaagatgaat acaaacgctc
    34741 aatgccgggc cgtattatcg gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat
    34801 ggcgatgcag actcgcgagc aacatattcg ccgtgagaaa gcgaactcca acatttgtac
    34861 ttcccaggtg ctgctggcaa acatcgccag cctgtatgcc gtttatcacg gcccgattgg
    34921 cctgaaacgt atcgctaacc gcattcaccg tctgaccgat atcctggcgg cgggcctgca
    34981 acaaaaaggt ctgaaactgc gccatgcgca ctatttcgac accttgtgtg tggaagtggc
    35041 cgacaaagcg ggcgtactga cgcgtgccga agcggctgaa atcaacctgc gtagcgatat
    35101 tctgaacgcg gttgggatca cccttgatga aacaaccacg cgtgaaaacg taatgcagct
    35161 tttcaacgtg ctgctgggcg ataaccacgg cctggacatc gacacgctgg acaaagacgt
    35221 ggctcacgac agccgctcta tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca
    35281 tccggtgttt aatcgctacc acagcgaaac cgaaatgatg cgctatatgc actcgctgga
    35341 gcgtaaagat ctggcgctga atcaggcgat gatcccgctg ggttcctgca ccatgaaact
    35401 gaacgccgcc gccgagatga tcccaatcac ctggccggaa tttgccgaac tgcacccgtt
    35461 ttgtccgccg gaacaggctg aaggttatca gcagatgatt gcgcagctgg ctgactggct
    35521 ggtgaaactg accggttacg acgccgtttg tatgcagccg aactccggcg cacagggcga
    35581 atacgcgggc ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga
    35641 tatctgcctg atcccggctt ctgcgcacgg aactaacccc gcttctgcac atatggcagg
    35701 aatgcaggtg gtggttgtgg cgtgtgataa aaacggcaac atcgatctga ctgatctgcg
    35761 cgcgaaagcg gaacaggcgg gcgataacct ctcctgtatc atggtgactt atccttctac
    35821 ccacggcgtg tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg
    35881 tcaggtttac cttgatggcg cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt
    35941 tattggcgcg gacgtttcgc acctcaacct gcataaaact ttctgcattc cgcacggcgg
    36001 tggtggtccg ggtatgggac cgatcggtgt gaaagcgcat ctggcaccgt ttgtaccggg
    36061 tcatagcgtg gtgcaaatcg aaggcatgtt aacccgtcag ggcgcagttt ctgcggcacc
    36121 gttcggtagc gcctctatcc tgccaatcag ctggatgtac atccgcatga tgggcgcaga
    36181 agggctgaaa aaagcaagcc aggtggcaat cctcaacgcc aactatattg ccagccgcct
    36241 gcaggatgcc ttcccggtgc tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat
    36301 tctcgatatt cgcccgctga aagaagaaac cggcatcagc gagctggata ttgccaagcg
    36361 cctgatcgac tacggtttcc acgcgccgac catgtcgttc ccggtggcgg gtacgctgat
    36421 ggttgaaccg actgaatctg aaagcaaagt ggaactggat cgctttatcg acgcgatgct
    36481 ggctatccgc gcagaaatcg accaggttaa agccggtgtc tggccgctgg aagataaccc
    36541 gctggtgaac gcgccgcaca ttcagaacga actggtcgcc gagtgggcgc atccgtacag
    36601 ccgtgaggtt gcagtattcc cggcaggtgt ggcagacaaa tactggccga cggtgaaacg
    36661 tctggatgat gtttacggcg accgtaacct gttctgctcc tgcgtaccga ttagcgaata
    36721 ccagtaattc actgattcga ctattttcta aaggcgcttc ggcgcctttt tagtcagatg
    36781 acaaagtaca aaagtgctca gacagtcccc tcgccccttt ggggagaggg tgaggggaac
    36841 aggccggcac tggcgcgaat atttaccctc accccggccc tctccctgaa agggcgaggg
    36901 ggaaaagcgt gccaacattg aagattgagc cagtttgtta gcaatctcaa agatacgtca
    36961 acgaatgaat ttttctcgga aaaacaaatg gttatagcac ttgtgaccgg tggcagtcgc
    37021 ggcatcgggc gggcaactgc attactgttg gcgcaagaag ggtatacggt ggcggttaat
    37081 tatcagcaaa acctccacgc ggcgcaggaa gtgatgaact taataacgca agccggtggc
    37141 aaagcattcg tgctccaggc ggatatcagc gacgaaaacc aggtcgttgc gatgtttaca
    37201 gcaatcgatc agcacgatga accgctagca gcgctggtca ataacgccgg gatcttgttt
    37261 acccagtgca ccgttgaaaa ccttaccgca gagcgaatca accgagtact ttccaccaac
    37321 gtgacgggat attttctctg ctgccgcgag gcggtaaaac gcatggcgct taaaaatggt
    37381 ggcagtggcg gcgctatcgt caatgtctct tcggtggcct cacggttggg ttcgccaggg
    37441 gaatatgttg attacgcggc atcgaaaggg gcgattgata cgttaaccac cggactatcg
    37501 ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc ggccagggtt tatttatacc
    37561 gaaatgcacg ccagcggcgg cgagcctgga cgcgtcgatc gcgttaagtc gaacatcccc
    37621 atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca ttgtctggct actaagtgat
    37681 aaagcctctt acgtcacggg aagttttatc gatttggcgg gcgggaaata aaacagggaa
    37741 gttgtctgac cggatgcaac aagtattgca tccggtactt catcgactta aagcttctcg
    37801 ccgttgctgg caatcacctc ttggtaccag ttaaagctct tcttacgtga acgcgacata
    37861 tcgccagcac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt gctgtactgc
    37921 ccggtggtga acgatacgca gtcgatgcag ccccacggtg tgtagcccat cagatccacg
    37981 ccatcgtaag tcaccgcttt tttcatctct tcgatatggg cgcgcaggta gtcaatgcgg
    38041 tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa accgttttcg
    38101 acaataaaca gcggcctctg ataacgctca tacagttcgc aaagggcata gcgcagacct
    38161 actggatcaa tctgccagcc ccagtcagac gctttaacat acgggttcgg tacgctgcct
    38221 tcgaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt ggtcatgtaa
    38281 tagctgaaac caagataatc gcaggtgcct tcacgcagta catcgagatc gccgtcttcc
    38341 attttgatgt taaatccgcg acgctcccac tcgttcaaca cataggacgg gtaatagccg
    38401 cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc gaacatcaca
    38461 tcgtccgggt tacagcagta gggatagagc ggcaccatcg ccagcataca gccgactttc
    38521 atctccgggt taatgcgacg cgcagctttc accgccaggg cgctggcgac aaactggtga
    38581 tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct cggtataaac cacgccggag
    38641 cagcagtaac cgaacagcgg tgcacgccag ttacgctggt tgttaatttc gttgaaggtc
    38701 atccagtatt tgactttatg cttatagcgt tcaaacacca cttccgcgaa acggacaaag
    38761 aaatcaacca ctttacggtt ggtccagcta ccgtattgct gcaccagatg cagcggcatt
    38821 tcaaagtggg agagggtgat caccggttcg atgttgtatt tcagcagttc atcgaacata
    38881 tcatcgtaga acttcagccc ttcttcgttt ggctgagctt catcaccttt tggaaaaata
    38941 cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca tttcggcaaa tagcttgatg
    39001 tcttccttgt agtgaccata aaaatcaacg gcttcatggt ttggatagta tttgcctggc
    39061 aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag aacgtcacaa
    39121 atgctcggcc ctttgccgcc tttgttccag ctgccttcga cctgatgagc ggcaactgcg
    39181 ccgccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg ctccagatga
    39241 gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt ttcaacgttt
    39301 ccaggatgac attctggctg ggcgtaaaac catcaccatc cgcgacgagt ctgaatcgca
    39361 cttcaaaacg ggtgatgtgc ttcgtgtcgg gcgttttgaa gatgacggtt atttttgcac
    39421 gattgaagtc accgcaacct caaccgtaag gctggatacg ctgactgaaa aacatgcaga
    39481 gcaggaaaat atgaccctga ctgaactgaa aaaggtcatt gccgacatct atcccggtca
    39541 gacacagttt tatgtgattg aatttaaatg tctttaaatt gaactcacgc ctgttagttg
    39601 aaatttctca tttaatttca tgatttttag gagattagtc tcaaatcaga atttatttta
    39661 gctaacaggt gttcactgga actattctca gttacgctgg agaggtatac atgtgcgtgt
    39721 acgtttctcc ggagtaagtt atggttcaga agcccctcat taagcaggga tattcgctgg
    39781 cagaggaaat tgccaacagc gtcagtcacg gcattgggtt ggtgtttggt atcgttgggc
    39841 tggtgttgct actggttcag gcggtggatc tcaatgccag cgccacagcg ataaccagct
    39901 acagcctcta tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc tatcacgcca
    39961 ttcctcatca acgggcaaaa atgtggctga agaaatttga ccattgcgct atttacctgt
    40021 tgattgccgg aacctacacg ccgtttttgc tggtggggct ggattctccg ttagcgcgcg
    40081 ggttgatgat tgttatctgg agcctggcat tgctggggat tctgtttaaa ctgaccatcg
    40141 cgcaccgatt caaaatttta tctctggtga cctatctggc gatgggctgg ctgtcgctgg
    40201 tggtaattta tgaaatggca gttaagctcg cggcgggcag cgttacctta ctggcggtag
    40261 gcggcgtggt ttattcgctc ggggtgattt tctacgtctg caaacgcatt ccatacaacc
    40321 atgccatctg gcacggcttc gtgctcggcg gtagtgtgtg ccactttctg gcgatctatt
    40381 tgtatattgg gcaggcgtaa tgggtatgcc tttgtagata ctgataagac gcgccagcgt
    40441 cgcatcaggc gataaatgcc tgatgcgata aagagattta ctcttcgagc gaatacggca
    40501 gcggctcgat atgcagtgta ttcgcatcgt cgcgtacgcg gaagatgcta tccggttcca
    40561 tatcgttatt catgacgacc tgtaccacga cctgaccatc ttccagtttt accgcagcca
    40621 gcaccgtacc ggtacgacgc cagttctcgc ccattttcag ctctaagtct tcaccagctt
    40681 ccggcagtcg gctggcgcta cctgccagca accagagcgc acgtttattg gcaccacgga
    40741 attttgctcg cgccaccatc tcttgtccgg tatagcagcc ttttttaaag ctgatgccgc
    40801 ccagcgcctg gaggttggtc gcctgtggga taaactgccc gctgttggcg gcatcaatca
    40861 ccgggaaacc cgcttcaatg tttaatgcca gccactgttg gctattgttc agttccgctt
    40921 caccgcgcag tttatcggtc agcatattag cagtagcttc atcggttacg atcaggaaac
    40981 gttctgccgg gtgttcaaac catagcaaag tggtcgcgcc ttctttaact acctgttttt
    41041 ctttcgaagg cagttcacta aagagatttg ccagcgcggc gcgcgcctga aaaccggcaa
    41101 caccaagcag cacacgctcg tcgtctggcg cgatggtcac tttagagaat accgcatatt
    41161 ttttcagttc agtcagctgc ggttcacgca cgctgcgccg ttcaatccat gcaaagccat
    41221 cgccgtcgcg gaacagacgt aaattgctcc acattttacc tttggcgtcg caatgggcgg
    41281 cgagcaggtg ctgatcttct gccatctggc tgacatctgc tgtcacctga ccctgcatat
    41341 atttttcgct gtccgcgcca gtaatggtgg caagcgccca gtcatcaagc gtcatcagcg
    41401 tcagcggtaa acgggcagaa gccgtaggct gacggggagg aaaaggtgta aaagccataa
    41461 tagagtcctg agttgcttaa cggcgtatta atgactaatg gtaaaagagc tatgaggcaa
    41521 tgcaagtgct ttcactacgc gatctgtgcc ttctgcgagg cgacttccaa aaaaatgaag
    41581 ccattgaatt gttgtgaatt aatttcgcga tcgcgatggt gtttactgat attcccgtaa
    41641 aagaaggcga aaaacacgtt acaatagccg ggtactggat tttcgtgaga caggaagaag
    41701 atggacatta acaacaaagc ccgcattcat tgggcatgcc gccgtggtat gcgcgaactc
    41761 gatatttcaa tcatgccgtt tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa
    41821 cgcatcttta ttcgtctgct ggaatgtgac gatccggacc tgtttaactg gctgatgaat
    41881 cacggtaaac cggccgatgc agaactggaa atgatggtcc gactcatcca gacacggaac
    41941 cgggaacgtg gtcctgtggc aatctgattt gcgcgtctcc tggcgcgcac agtggctttc
    42001 cttgctgatt catgggctgg ttgccgctgt tattttactc atgccctggc cactcagtta
    42061 caccccgtta tggatggtgt tactttcgct ggtggtgttt gattgcgttc gcagccagcg
    42121 gcgtattaat gctcgccagg gggaaattcg cttgttgatg gacgggcgtt tgcgttggca
    42181 agggcaggag tggtgcatcg tcaaagcacc gtggatgatt aagagcggca tgatgctgcg
    42241 tttacgttct gatggcggta aacggcaaca tttatggctg gcagccgaca gcatggacga
    42301 agccgaatgg cgggatttac ggcggatttt gttgcaacaa gagacgcaaa gataaagatg
    42361 acggcaaagc atatgcgcta agccgtcagt gagatcaggc gtaatgctct gccatttcgt
    42421 tgaggatttg ctcgcaccag ctctgaatac gctcgtcgct aaggtcatac tggttagttt
    42481 catccagcgc cagacccacg aacagttgcc cgtcagcaat caccggtttc gggctggtaa
    42541 attcatatcc ttccgttggc cagtagccga cgaacttcac gcctttggtc gagagtttgt
    42601 catgcagcat accgagcgca tcgaggaacc actcgccgta tcccagttga tcgccaagcc
    42661 catacagcgc aacaatttta ccttcaaggt tcaggtcgtc gagctgatcc cagacggctt
    42721 cccagtcttc ctggatttca ccaaaatccc aggtcgggat acccagaatg agcacatcgt
    42781 actgctccat taatttcggg gagtcgtcct tgaggttatg taaggtcacc agttctgggc
    42841 cgataatatc gcggattttt tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa
    42901 aaagacccat attcataacg taagcttctc gattctgctg tggcttttgc agctagtgta
    42961 tcagaatcgc tttagtcacg gcacaataag gcataatgga tcaaatgagt gaccataagg
    43021 ggcgcaagtg aaacaggatc tggcacgcat cgagcagttt cttgatgctc tgtggctgga
    43081 aaaaaatctg gctgaaaata cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga
    43141 gtggttgcat caccgcgggt tgacgctggc gacggcgcaa agtgacgatt tgcaggcatt
    43201 actggcagaa cggctggagg gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc
    43261 agtgcgccga ttgttccagt atctttatcg cgaaaagttt cgtgaagacg atcccagtgc
    43321 gcatctcgct tcaccgaaat tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt
    43381 cgaacgttta ttacaggcac cattaattga tcagccactg gagctacgcg ataaagccat
    43441 gcttgaagtg ttgtatgcta ccgggctgcg tgtctctgaa ctggtcggac tgacaatgag
    43501 tgatatcagc ctgcgtcagg gcgtagtgcg ggtcattggt aaaggcaaca aagagcgcct
    43561 ggtaccgtta ggtgaagagg cggtttactg gctggaaacc tatctggaac atgggcgtcc
    43621 gtggctgctg aatggtgtgt caattgacgt gttgtttccc agccagcgtg cgcagcagat
    43681 gacgcgacag accttctggc accgcattaa acattatgct gtgctggcgg gtatcgacag
    43741 cgaaaagctg tcaccgcatg tattgcgtca cgcttttgcc acccatttat taaatcatgg
    43801 tgcggattta cgcgtggtgc agatgctact gggccacagc gacctctcca ccacgcaaat
    43861 ttatacgcat gtcgctaccg agcgtctgcg gcaacttcat caacagcatc acccgcgggc
    43921 gtgatgtctg aaaagaacgg gaagatttat gaagaaaggt tttatgttgt ttactttgtt
    43981 agcggcgttt tcaggttttg ctcaggctga tgacgcggca attcaacaaa cgttagccaa
    44041 aatgggcatc aaaagcagcg atattcagcc cgcgcccgta gctggcatga agacagttct
    44101 gactaacagc ggcgtgttgt acatcaccga tgatggtaaa catatcattc aggggccaat
    44161 gtatgacgtt agcggcacgg ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt
    44221 gaatgcgctt gaaaaagaga tgatcgttta taaagcgccg caggaaaaac acgtcatcac
    44281 cgtgtttact gatattacct gtggttactg ccacaaactg catgagcaaa tggcagacta
    44341 taacgcgctg gggatcaccg tgcgttatct tgctttcccg cgccaggggc tggacagcga
    44401 tgcagagaaa gaaatgaaag ctatctggtg tgcgaaagat aaaaacaaag cgtttgatga
    44461 tgtgatggca ggtaaaagtg ccgcaccagc cagctgcgac gtggatattg ccgaccatta
    44521 cgcacttggc gtccagcttg gcgttagcgg tactccggca gttgtgctga gcaatggcac
    44581 acttgttccg ggttaccagc cgccgaaaga gatgaaagaa ttcctcgacg aacaccaaaa
    44641 aatgaccagc ggtaaataat tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc
    44701 gatgaaacgg cagacttgcc cgctgaattg cctcccttgc tgcgccgttt atacgccagc
    44761 cggggcgtgc gcagtgcgca agaactggaa cgcagtgtta aaggtatgtt gccctggcag
    44821 caactgagcg gtgtcgaaaa ggccgttgag atcctttaca acgcttttcg cgaaggaacg
    44881 cggattattg tggtcggtga tttcgacgcc gacggcgcga ccagcacggc tctaagcgtg
    44941 ctggcgatgc gctcgcttgg ttgcagcaat atcgactacc tggtaccaaa ccgtttcgaa
    45001 gacggttacg gcttaagccc ggaagtggtc gatcaggccc atgcccgtgg cgcgcagtta
    45061 attgtcacgg tggataacgg tatttcctcc catgcggggg ttgtacacgc tcgctcgttg
    45121 ggcattccgg ttattgttac cgatcaccat ttgccgggcg aaacattacc cgcagcggaa
    45181 gcgatcatta accctaactt gcgcgactgt aatttcccgt cgaaatcact ggcaggcgtg
    45241 ggtgtggcgt tttatctgat gctggcgctg cgcacctttt tgcgcgatca gggctggttt
    45301 gatgagcgtg gcatcgcaat tcctaacctg gcagaactgc tggatctggt cgcgctggga
    45361 acagtggcgg acgtcgtgcc gctggacgct aataatcgca ttctgacctg gcaggggatg
    45421 agtcgcatcc gtgccggaaa gtgccgtcca gggattaaag cgctgctgga agtggcaaac
    45481 cgtgatgcac aaaaactcgc cgccagcgat ttaggttttg cgctggggcc acgtctcaat
    45541 gctgccggac gactggacga tatgtccgtc ggtgtggcgc tcttgctgtg cgacaacatc
    45601 ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa accagacgcg aaaagagatc
    45661 gaacaaggaa tgcaagttga agccctgacc ctgtgcgaga aactggagcg aagtcgcgac
    45721 acgctacccg gcgggctggc aatgtatcac cccgaatggc atcagggcgt tgtcggtatt
    45781 ctggcttcgc gcatcaaaga gcgttttcac cgtccggtta tcgcctttgc gccagcaggt
    45841 gatggtacgc tgaaaggttc aggtcgctcc attcaggggc tgcatatgcg tgatgcactg
    45901 gagcgattag acacactcta ccctggcatg atgctgaagt ttggcggtca tgcgatggcg
    45961 gcgggtttgt cgctggaaga ggataaattc gaactctttc aacaacggtt tggcgaactg
    46021 gttactgagt ggctggaccc ttcgctattg caaggcgaag tggtgtcaga cggtccgtta
    46081 agcccggccg aaatgaccat ggaagtggcg cagctgctgc gcgatgctgg cccgtggggg
    46141 cagatgttcc cggagccgct gtttgacggt catttccgtc tgctgcaaca gcggctggtg
    46201 ggcgaacgtc atttgaaggt gatggtcgaa ccggtcggcg gcggtccact gctggatggt
    46261 attgctttta atgtcgatac cgctctctgg ccggataacg gcgtgcgcga agtgcaactg
    46321 gcttacaagc tcgatatcaa cgagtttcgc ggcaaccgca gcctgcaaat tatcatcgac
    46381 aatatctggc caatttagcg tcatcttctc tataaaaaag agcgtggatt gggtacaatc
    46441 ccgctcttat caccgcattt tgactagctc aataaaagaa atcagaccat gtttgaaatt
    46501 aatccggtaa ataatcgcat tcaggacctc acggaacgct ccgacgttct tagggggtat
    46561 ctttgactac gacgccaaga aagagcgtct ggaagaagta aacgccgagc tggaacagcc
    46621 ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt aaagagcgtt cctccctcga
    46681 agccgttgtc gacaccctcg accaaatgaa acaggggctg gaagatgttt ctggtctgct
    46741 ggaactggct gtagaagctg acgacgaaga aacctttaac gaagccgttg ctgaactcga
    46801 cgccctggaa gaaaaactgg cgcagcttga gttccgccgt atgttctctg gcgaatatga
    46861 cagcgccgac tgctacctcg atattcaggc ggggtctggc ggtacggaag cacaggactg
    46921 ggcgagcatg cttgagcgta tgtatctgcg ctgggcagaa tcgcgtggtt tcaaaactga
    46981 aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa tccgtgacga tcaaaatctc
    47041 cggcgattac gcttacggct ggctgcgtac agaaactggc gttcaccgcc tggtgcgtaa
    47101 gagcccgttt gactccggcg gtcgtcgcca cacgtcgttc agctctgcgt ttgtttaccc
    47161 ggaagttgat gatgatattg atatcgaaat caacccggcg gatttgcgca ttgacgttta
    47221 tcgcgcgtcc ggcgcgggcg gtcagcacgt taaccgtacc gaatctgcgg tgcgtattac
    47281 ccacatcccg accgggatcg tgacccagtg ccagaacgac cgttcccagc acaagaacaa
    47341 agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa ctggagatgc agaagaaaaa
    47401 tgccgagaaa caggcgatgg aagataacaa atccgacatc ggctggggca gccagattcg
    47461 ttcttatgtc cttgatgact cccgcattaa agatctgcgt accggggtag aaacccgcaa
    47521 cacgcaggcc gtgctggacg gcagcctgga tcaatttatc gaagcaagtt tgaaagcagg
    47581 gttatgagga accaacatgt ctgaacaaca cgcacagggc gctgacgcgg tagtcgatct
    47641 taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac ctgcgcgagc aggggattgc
    47701 cttcccgaac gatttccgtc gcgatcatac ctctgaccaa ttgcacgcag aattcgacgg
    47761 taaagagaac gaagaactgg aagcgctgaa catcgaagtc gccgttgctg gccgcatgat
    47821 gacccgccgt attatgggta aagcgtcttt cgttaccctg caggatgtgg gtggccgcat
    47881 tcagctgtac gttgcccgtg acgatctgcc ggaaggcgtt tacaacgagc agttcaaaaa
    47941 atgggacctc ggcgacatcc tcggcgcgaa aggtaaactg ttcaaaacca aaaccggcga
    48001 actgtctatc cactgtaccg agctgcgtct gctgaccaaa gcactgcgtc cgctgccgga
    48061 taaattccac ggcttgcagg atcaggaagc gcgctatcgt cagcgttatc tggatctcat
    48121 ctctaacgat gaatcccgta acacctttaa agtacgctcg cagatcctct ctggtattcg
    48181 ccagttcatg gtgaaccgcg gctttatgga agttgaaacg ccgatgatgc aggtgatacc
    48241 aggcggtgcc gctgcgcgtc cgtttatcac ccaccataac gcgctggatc tcgacatgta
    48301 cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg gtcggtggct tcgagcgtgt
    48361 attcgaaatc aaccgtaact tccgtaacga aggtatttcc gtacgtcata acccagagtt
    48421 caccatgatg gaactctaca tggcttacgc agattacaaa gatctgatcg agctgaccga
    48481 atcgctgttc cgtactctgg cacaggatat tctcggtaag acggaagtga cctacggcga
    48541 cgtgacgctg gacttcggta aaccgttcga aaaactgacc atgcgtgaag cgatcaagaa
    48601 atatcgcccg gaaaccgaca tggcggatct ggataacttc gactctgcga aagcgattgc
    48661 tgaatctatc ggtatccacg ttgagaagag ctggggtctg ggccgtatcg ttaccgagat
    48721 cttcgaagaa gtggcagaag cacatctgat tcagccgacc ttcattactg aatatccggc
    48781 agaagtttct ccgctggcgc gtcgtaacga cattaacccg gaaatcacag accgctttga
    48841 gttcttcatc ggtggtcgtg aaatcggtaa cggctttagc gagctgaatg acgcggaaga
    48901 tcaggcgcaa cgcttcctgg atcaggttgc cgcgaaagac gcaggtgacg acgaagcgat
    48961 gttctacgat gaagattacg ttaccgcact ggaacatggc ttaccgccga cagcaggtct
    49021 ggggattggc atcgaccgta tggtaatgct gttcaccaac agccatacca tccgcgacgt
    49081 tattctgttc ccggcgatgc gtccggtaaa ataagcatta cgttatgctc acaaccccgg
    49141 caaatgtcgg ggttttttaa ttgtgctgcg cgaaagcaga caacaatttt cttgcttcac
    49201 tattggctgc ctgcatcacc atccacggac tgaacgccca cggtgtggca tcaataccgt
    49261 gtaatacatc tgctaaatca caccattgat aatccatcac ttcatcatcg ttgatctgta
    49321 acgcactggt ggtgcgtgcg gcaaataccg gacacacttc attttccaca atgccattcg
    49381 gatcggtggc gcgatagcga aagtcaggat agatagattc aggaggcgta atttccacgc
    49441 caagctcata acggcaacgg cggatcaccg cgtcttcgtt gctttctccc agttgtgggt
    49501 gcccacaaac cgagttagtc cacacgccag gccacgcttt tttgctcagt gcgcggcggg
    49561 taactaataa ttgtcctttg gcattaaaca gccaactgga gaacgcgaga tgtaagcggg
    49621 tgtctgccgt gtgtgcggca tacttttcca gcgtaccagt gggaactccc tgtgcattca
    49681 ataaaatgac gtgttccgtt tgcataattt ctcacatgta attctgatca ctcatcatta
    49741 tagataattg aagagtgaaa tgattaacca agtgagcgtt tatcgacaac cgcccgtttt
    49801 gagcggatgc cgacaggtaa aaaccattta aaaaagtagc atcatatatt tgaagcgact
    49861 ttcaatggcc attgcaacac agtcagaaat gacaggaata gagagagtaa aaagcaacag
    49921 cagactgaat atagcgcgca ttaaaaaatt catattattc tccatgctca tttttcttca
    49981 gcttgcgcat cagaagccac atacatactg tcctgacttt taaatcttta tcagagacgg
    50041 attccttcac caggtcagca tgatgctgat agccattaaa gaagacattc agcacaacgg
    50101 cgctaagggt tgcgagcata atgccgctat gcagcaacgg ttgcagtacg gccggtaact
    50161 tagaaaagaa atcgtgagag agcgtcggag tcatgccaac gccgagactg atcgccacaa
    50221 tatagaggtt gtaacggttg gtggtgtagt tacagcgcga cagaattcga atccctgtcg
    50281 ccagtaccat gccgaacatc accagcccag cgccgcccag cacaaattgc ggaatggagg
    50341 ctaccagcac cgccattttt ggcaccatgc caaacaggat taatataatt cccgaagaaa
    50401 tacacaccca gcggctatga acgcgcgtca cgctaaccag gccaacgttt tgagaaaatg
    50461 acgtgtgggg gaagctgtta aacgtgccgc ctatcattgt ccctacgcca tcgacacgca
    50521 gcccgcgaat aatatcgtgt gaagagagtt tacgaccgac tatttcaccc agtgccagga
    50581 acatccccat cgactcgata aacacgatga ttaacacggc agtcatggtc agaatggaaa
    50641 cgggatcgaa aatcggcata ccaaacgaca tcggggtgac aatcgcaaac cacgaggcat
    50701 catgtagccc ggataaattg acttcattca tcatccacga aagtaaaaag ccaaatacaa
    50761 tccccagtaa tacggcgacg ttggacataa atcctttcgc atagcgagta atgagcaaga
    50821 taaaaattaa gacggcaaag gagataccta aataaacggg attaccatat tgcggattcc
    50881 ctttacctcc ggcggcccag tcaataccca cctgaatgat gctaagccca atagaagtaa
    50941 taaccacacc ggtaaccagt ggcgggaata aaggcatcaa gcgaccaata agtggcgcta
    51001 ataatgtggt gataaaaccc gcggcgatag tggcaccaaa tatccccagc aggccgatat
    51061 ccgggttcat cccaatggct atcattggtg ttacagcagc aaaggtcacc gacataatca
    51121 ccggcaggcg gatccccata aagcggccga taccgataca ttgcaataat gtgacgatcc
    51181 cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc ttcttttgag aggcccagtc
    51241 ggtcaccaat cataagagga acagcgactg cacctgcgta catgacaagg acgtgttgca
    51301 gaccgagtat gatcagcttt cctggtgata atatgcggtc aacctcatca gttgggcggt
    51361 cttgcccaga agatgaggga agttgggaat ctatggcgct catcagtgtt tctcctttcg
    51421 cccgccagag taaaaagcaa ccgtttacgt taatgaaact gtctaaacac atgctcgtgg
    51481 agtgccagtt tttgcgagga aggggagtta gagacaggaa ttgcggaggc aatcacataa
    51541 tcgtgcttaa accacgaaaa tgagaagaga acctgacaat gaaattatct gtaattttct
    51601 catattgata tttcattaat cattctggca tgtcgcactc tcgcatttaa tcgtttttat
    51661 ctggatagcg ctcttttggt cggcaatgtt gaaataatga aagcggcata gattgagggg
    51721 caataatttg aactcagtcg caaaattata attaaaacgg tcatgctacc taatatatgc
    51781 ccgtttattg catcgcgaaa tttgagaaga cataactcat taaatgaaag ggatgcaaat
    51841 gaataagttt atcgctgctg aagctgcgga atgtataggc tgtcatgctt gtgaaattgc
    51901 ctgtgcggtg gcacacaatc aagaaaactg gccgctgagt cacagtgact ttcgaccgcg
    51961 tatccacgtt gtagggaaag gccaggctgc gaatccggtg gcctgccatc actgcaacaa
    52021 tgccccttgc gttacggctt gtccggttaa tgctctgact ttccagtccg atagcgtaca
    52081 actggacgag caaaaatgta ttggctgtaa aagatgtgca atcgcttgcc cctttggcgt
    52141 cgttgagatg gtcgatacga ttgcgcagaa atgcgacctt tgtaaccagc gcagttccgg
    52201 cacgcaagcc tgtattgaag tctgcccaac acaggcgtta cgactgatgg acgataaagg
    52261 gttacagtgg ataaaggtgg ctcgccagcg caaaacggca gcaggaaaag cgtcatcaga
    52321 tgctcagcca tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag gcgcagataa
    52381 aatttcagcg agtgaacgga aaacccactt tggcgaaata tattgcgggc tggatccact
    52441 gcaagcgact tatgagagtg accgctgtgt ttattgtgcc gaaaaagcta actgcaactg
    52501 gcattgcccg ctgcataacg ctattccgga ttacatccgt ctggtacagg aaggaaagat
    52561 tattgaagcg gcagaacttt gccaccagac cagttcctta ccagaaattt gcggcagggt
    52621 atgtccacag gaccgtcttt gtgaaggcgc atgtactttg aaagatcact cgggcgcagt
    52681 ctctatcggt aatctggaac gctacatcac cgataccgcg ctggcgatgg gctggcgtcc
    52741 cgatgtcagc aaagttgttc cccgtagcga aaaagtggcg gtgattggcg ctgggccagc
    52801 agggttaggg tgtgctgata ttctggcgcg cgcgggagtt caggttgatg tctttgatcg
    52861 ccatccagaa attgg
//
LOCUS       DAZFYC010000019        48720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFYC010000019 DAZFYC010000000
VERSION     DAZFYC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.019"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            370..1587
                     /gene="alaA"
                     /locus_tag="ACHKW5_001300"
     CDS             370..1587
                     /gene="alaA"
                     /locus_tag="ACHKW5_001300"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO7510729.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1671..2270
                     /gene="yfbR"
                     /locus_tag="ACHKW5_001301"
     CDS             1671..2270
                     /gene="yfbR"
                     /locus_tag="ACHKW5_001301"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7510730.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2329..4161)
                     /locus_tag="ACHKW5_001302"
     CDS             complement(2329..4161)
                     /locus_tag="ACHKW5_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO7510731.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4248..4898)
                     /gene="hxpA"
                     /locus_tag="ACHKW5_001303"
     CDS             complement(4248..4898)
                     /gene="hxpA"
                     /locus_tag="ACHKW5_001303"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO7510732.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     LEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4909..5403)
                     /locus_tag="ACHKW5_001304"
     CDS             complement(4909..5403)
                     /locus_tag="ACHKW5_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO7510733.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5486..5941)
                     /gene="yfbV"
                     /locus_tag="ACHKW5_001305"
     CDS             complement(5486..5941)
                     /gene="yfbV"
                     /locus_tag="ACHKW5_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO7510734.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6279..7481
                     /gene="ackA"
                     /locus_tag="ACHKW5_001306"
     CDS             6279..7481
                     /gene="ackA"
                     /locus_tag="ACHKW5_001306"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO7510735.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7556..9700
                     /gene="pta"
                     /locus_tag="ACHKW5_001307"
     CDS             7556..9700
                     /gene="pta"
                     /locus_tag="ACHKW5_001307"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7510736.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAIVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9890..11410
                     /gene="yfcC"
                     /locus_tag="ACHKW5_001308"
     CDS             9890..11410
                     /gene="yfcC"
                     /locus_tag="ACHKW5_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO7510737.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11443..11985)
                     /gene="yfcD"
                     /locus_tag="ACHKW5_001309"
     CDS             complement(11443..11985)
                     /gene="yfcD"
                     /locus_tag="ACHKW5_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO7510738.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12043..12594)
                     /gene="yfcE"
                     /locus_tag="ACHKW5_001310"
     CDS             complement(12043..12594)
                     /gene="yfcE"
                     /locus_tag="ACHKW5_001310"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO7510739.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12650..13294)
                     /gene="yfcF"
                     /locus_tag="ACHKW5_001311"
     CDS             complement(12650..13294)
                     /gene="yfcF"
                     /locus_tag="ACHKW5_001311"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO7510740.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13430..14077
                     /gene="yfcG"
                     /locus_tag="ACHKW5_001312"
     CDS             13430..14077
                     /gene="yfcG"
                     /locus_tag="ACHKW5_001312"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO7510741.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14134..14496
                     /gene="folX"
                     /locus_tag="ACHKW5_001313"
     CDS             14134..14496
                     /gene="folX"
                     /locus_tag="ACHKW5_001313"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO7510742.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14517..15410
                     /locus_tag="ACHKW5_001314"
     CDS             14517..15410
                     /locus_tag="ACHKW5_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO7510743.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15458..16348)
                     /gene="rpnB"
                     /locus_tag="ACHKW5_001315"
     CDS             complement(15458..16348)
                     /gene="rpnB"
                     /locus_tag="ACHKW5_001315"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO7510744.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIAHKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16545..17318)
                     /gene="hisP"
                     /locus_tag="ACHKW5_001316"
     CDS             complement(16545..17318)
                     /gene="hisP"
                     /locus_tag="ACHKW5_001316"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO7510745.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17326..18042)
                     /locus_tag="ACHKW5_001317"
     CDS             complement(17326..18042)
                     /locus_tag="ACHKW5_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7510746.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18039..18725)
                     /gene="hisQ"
                     /locus_tag="ACHKW5_001318"
     CDS             complement(18039..18725)
                     /gene="hisQ"
                     /locus_tag="ACHKW5_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO7510747.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18815..19597)
                     /gene="hisJ"
                     /locus_tag="ACHKW5_001319"
     CDS             complement(18815..19597)
                     /gene="hisJ"
                     /locus_tag="ACHKW5_001319"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO7510748.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19818..20600)
                     /gene="argT"
                     /locus_tag="ACHKW5_001320"
     CDS             complement(19818..20600)
                     /gene="argT"
                     /locus_tag="ACHKW5_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO7510749.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20866..21435)
                     /gene="ubiX"
                     /locus_tag="ACHKW5_001321"
     CDS             complement(20866..21435)
                     /gene="ubiX"
                     /locus_tag="ACHKW5_001321"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO7510750.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21530..23047)
                     /gene="purF"
                     /locus_tag="ACHKW5_001322"
     CDS             complement(21530..23047)
                     /gene="purF"
                     /locus_tag="ACHKW5_001322"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO7510751.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23084..23572)
                     /gene="cvpA"
                     /locus_tag="ACHKW5_001323"
     CDS             complement(23084..23572)
                     /gene="cvpA"
                     /locus_tag="ACHKW5_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO7510752.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23831..24493)
                     /gene="dedD"
                     /locus_tag="ACHKW5_001324"
     CDS             complement(23831..24493)
                     /gene="dedD"
                     /locus_tag="ACHKW5_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO7510753.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24483..25751)
                     /gene="folC"
                     /locus_tag="ACHKW5_001325"
     CDS             complement(24483..25751)
                     /gene="folC"
                     /locus_tag="ACHKW5_001325"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO7510754.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25821..26735)
                     /gene="accD"
                     /locus_tag="ACHKW5_001326"
     CDS             complement(25821..26735)
                     /gene="accD"
                     /locus_tag="ACHKW5_001326"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO7510755.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26891..27550)
                     /locus_tag="ACHKW5_001327"
     CDS             complement(26891..27550)
                     /locus_tag="ACHKW5_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7510756.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27633..28445)
                     /gene="truA"
                     /locus_tag="ACHKW5_001328"
     CDS             complement(27633..28445)
                     /gene="truA"
                     /locus_tag="ACHKW5_001328"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO7510757.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28445..29458)
                     /locus_tag="ACHKW5_001329"
     CDS             complement(28445..29458)
                     /locus_tag="ACHKW5_001329"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7510758.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29524..30660)
                     /gene="pdxB"
                     /locus_tag="ACHKW5_001330"
     CDS             complement(29524..30660)
                     /gene="pdxB"
                     /locus_tag="ACHKW5_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO7510759.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30759..31754
                     /gene="flk"
                     /locus_tag="ACHKW5_001331"
     CDS             30759..31754
                     /gene="flk"
                     /locus_tag="ACHKW5_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO7510760.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31751..32929)
                     /locus_tag="ACHKW5_001332"
     CDS             complement(31751..32929)
                     /locus_tag="ACHKW5_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510761.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33213..34433)
                     /gene="fabB"
                     /locus_tag="ACHKW5_001333"
     CDS             complement(33213..34433)
                     /gene="fabB"
                     /locus_tag="ACHKW5_001333"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO7510762.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34592..36598
                     /gene="mnmC"
                     /locus_tag="ACHKW5_001334"
     CDS             34592..36598
                     /gene="mnmC"
                     /locus_tag="ACHKW5_001334"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO7510763.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36720..36998)
                     /locus_tag="ACHKW5_001335"
     CDS             complement(36720..36998)
                     /locus_tag="ACHKW5_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO7510764.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37032..37580)
                     /gene="epmC"
                     /locus_tag="ACHKW5_001336"
     CDS             complement(37032..37580)
                     /gene="epmC"
                     /locus_tag="ACHKW5_001336"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO7510765.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37580..38389)
                     /locus_tag="ACHKW5_001337"
     CDS             complement(37580..38389)
                     /locus_tag="ACHKW5_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO7510766.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38389..39213)
                     /gene="mepA"
                     /locus_tag="ACHKW5_001338"
     CDS             complement(38389..39213)
                     /gene="mepA"
                     /locus_tag="ACHKW5_001338"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO7510767.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39217..40302)
                     /gene="aroC"
                     /locus_tag="ACHKW5_001339"
     CDS             complement(39217..40302)
                     /gene="aroC"
                     /locus_tag="ACHKW5_001339"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO7510768.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40337..41269)
                     /gene="prmB"
                     /locus_tag="ACHKW5_001340"
     CDS             complement(40337..41269)
                     /gene="prmB"
                     /locus_tag="ACHKW5_001340"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7510769.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41435..41986
                     /gene="smrB"
                     /locus_tag="ACHKW5_001341"
     CDS             41435..41986
                     /gene="smrB"
                     /locus_tag="ACHKW5_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO7510770.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42108..42980)
                     /locus_tag="ACHKW5_001342"
     CDS             complement(42108..42980)
                     /locus_tag="ACHKW5_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO7510771.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42967..43491)
                     /locus_tag="ACHKW5_001343"
     CDS             complement(42967..43491)
                     /locus_tag="ACHKW5_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510772.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43488..43958)
                     /locus_tag="ACHKW5_001344"
     CDS             complement(43488..43958)
                     /locus_tag="ACHKW5_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510773.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43955..44503)
                     /locus_tag="ACHKW5_001345"
     CDS             complement(43955..44503)
                     /locus_tag="ACHKW5_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510774.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44478..45230)
                     /locus_tag="ACHKW5_001346"
     CDS             complement(44478..45230)
                     /locus_tag="ACHKW5_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7510775.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45250..47892)
                     /gene="yfcU"
                     /locus_tag="ACHKW5_001347"
     CDS             complement(45250..47892)
                     /gene="yfcU"
                     /locus_tag="ACHKW5_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO7510776.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVANNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(47974..48537)
                     /locus_tag="ACHKW5_001348"
     CDS             complement(47974..48537)
                     /locus_tag="ACHKW5_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7510777.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12080 a        13111 c        12023 g        11506 t
ORIGIN      
        1 gtttcacacc ttgcccccat tgcgacacct cggaaatcat cacgctgtta aactctccgt
       61 aatgcggaaa tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac
      121 aattatctta cagataaaaa aaccagactt acaattaaga atcagaacaa ttcactatat
      181 aacattgcat gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca
      241 caatcgagcc ttccactctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg
      301 gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg
      361 tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc
      421 cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa
      481 ctgaacatcg gcaacccagc cccgttcggt tttgacgcgc cagatgaaat cctcgttgac
      541 gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaagggct ttactccgcg
      601 cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac cgtggaagat
      661 atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac
      721 agcggggacg aaatgttggt tcctgcacca gattacccac tatggaccgc ggcggtttcg
      781 ctttccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac
      841 ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggatcgttat tatcaaccca
      901 aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca
      961 cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgac
     1021 gctgagcatc actcaattgc gccgctggca cctgacctgc tgaccattac ctttaacgga
     1081 ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg
     1141 aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt
     1201 gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt
     1261 gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaactgatc
     1321 aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa
     1381 atcgacgcca aacgctttaa cattcacgac gatcagaaaa tggtgctgga tttcctgttg
     1441 caggaaaaag tcctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc
     1501 cgcattgtca ctctgccgcg tgtcgatgat atcgagctgt ctttgagtaa gttcgcgcgt
     1561 ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca
     1621 gtgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga
     1681 gccatttttt tgcccatctc tcacgcctga aactcattaa ccgctggccg ctaatgcgca
     1741 acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc
     1801 tggcagctat caaaaatcgt aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac
     1861 tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat
     1921 acttcaattc gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcaaaaac
     1981 tggtcgatat ggttccggaa gagctgcagg atatctttgc gccgttaatt gacgagcatg
     2041 catatagcga tgaagaaaaa tcgttggtga aacaggcaga tgcactgtgt gcatatctga
     2101 aatgtctgga agaactcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg
     2161 aagcgacgct tgaagcgcgt cgcagccagg agatggacta cttcatggaa gtatttgttc
     2221 ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa gcagccggag
     2281 tctgcgtcgc atcaggcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa
     2341 cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccaa
     2401 cttcacaaag tcactgaagc tgtaatttcc cggacctaaa accagtgtgt taaccggtga
     2461 agaaaccggt gtcataaagg cggcagatgc tgccatcgcc acgaccatcg cgaatggata
     2521 aggcgacacc cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt
     2581 cgcggtatta gagataaaca gcccaataac cgccgacaaa acaaacaaac accccagcat
     2641 catatgtggc ccgtaaccgc cgccaatgtc catcagccct ttcaccgcca gcgcgacacc
     2701 tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg
     2761 ccagtgaatg gatttatagg cgctttcggc atcgatacag cggaatttcc ccatcagcag
     2821 gcaggcgatg atagcggcaa caggattagg aatttcatcg gtcagcatta acgccaccat
     2881 tagcaccaga cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac
     2941 ctcttccggt aagttcagcg ctacgaagtc gcggccctgt tttgccagca taccgatcag
     3001 tttccagtta ccaacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga
     3061 accttccagc gccacgccat tgcgctttag ccccaccaca ttcagtccgt agcgggtacg
     3121 aaaaccaatt tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc
     3181 tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc
     3241 cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag
     3301 caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa
     3361 acgccgccag cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc
     3421 gtccagccgt tgaccaatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc
     3481 ggtcagtcga tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg
     3541 cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag
     3601 caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc
     3661 acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc
     3721 gctaatcagc ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg
     3781 catggcaacg cttaacacca cgggaataaa gatagcgaca acgccggttg aactcataaa
     3841 cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc
     3901 cgccactttg accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc
     3961 gccaataata aacaaggcgg caatcaggac aacgttagga tcagaaaagc cggaaaatac
     4021 ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc
     4081 atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac
     4141 ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg
     4201 ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata
     4261 acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag gaccaaatcg
     4321 acctcattca ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc
     4381 gccaggccag aaagcacgcc agcgggagca tcttccagca ccacacactc ctgcggcgca
     4441 agccccagca gctgcgcacc taacagatac gcatcaggtt ctggttttcc gcgcttcact
     4501 cgctcagcgg ttacaaacac ctctggtgcg ggaagcccag ctattttatg gcgcgctcgc
     4561 gctaccggca tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga
     4621 ttgagtaagg cgattgcccc cggaagcgcg gtaatacctt cggtttccgt ggcctcgatg
     4681 tgctccagac gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg
     4741 cgcaaagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggtgctaac
     4801 ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc
     4861 gttccatcaa gatcaaacag aaaacctttg caccgcacac gggcctcctc aggcgttgat
     4921 aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt
     4981 aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccgt gagttccggc
     5041 gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg
     5101 tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg
     5161 gtcctgcaaa ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat
     5221 agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccaattcac gcatctgtaa
     5281 tccgtaacca cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc
     5341 gagcatagtc atcatcttgt actgattaga caaaataaga cgttgcgcgt tggtcatttc
     5401 cattgttgac tcctgtatca ctatactacg gtgaaaaaaa agaaggctga gtatgccttc
     5461 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
     5521 tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
     5581 ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttaaggattg
     5641 cagggggtaa tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac
     5701 ttaaggcgaa cagcgcagtg gcaactgccg gcccaagctg accgcccagg gcaatctgcc
     5761 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
     5821 taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagcggccac gtctttgagt
     5881 aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
     5941 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
     6001 ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
     6061 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
     6121 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
     6181 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
     6241 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
     6301 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
     6361 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
     6421 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
     6481 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
     6541 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
     6601 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc
     6661 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
     6721 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt
     6781 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
     6841 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
     6901 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
     6961 cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
     7021 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
     7081 aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
     7141 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
     7201 accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg
     7261 ttgtattcac tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
     7321 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
     7381 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
     7441 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
     7501 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
     7561 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
     7621 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
     7681 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
     7741 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
     7801 tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc
     7861 tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct
     7921 gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac
     7981 tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggcggtgc
     8041 caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca
     8101 gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctatagtaaa
     8161 caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc
     8221 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
     8281 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
     8341 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
     8401 ccgtcctgac gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc
     8461 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
     8521 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag
     8581 cctgcagagc ttcaacctgg aagttccggt tgacgatcac gagcgtatcg agaaagttca
     8641 ggaatacgtt gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga
     8701 gcgcagccgt cgtctgtctc cgcctgcgtt ccgttatcag ctgactgaac ttgcgcgcaa
     8761 agcgggcaaa cgtatcgtac tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
     8821 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa
     8881 ccgtgttgca gcgtctcagg gtgtagaact gggtgcaggg attgaaatcg ttgatccaga
     8941 agtggttcgc gaaagctatg ttggtcgtct ggtcgaactg cgtaagaaca aaggcatgac
     9001 cgaaaccgtt gcccgcgaac agttggaaga caacgtggtg ctcggtacgc tgatgctgga
     9061 acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg
     9121 tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
     9181 catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
     9241 cgctgaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat
     9301 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt
     9361 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga
     9421 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa
     9481 ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac
     9541 cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg
     9601 tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac
     9661 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccgcagct
     9721 ttgcgctgcg gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat
     9781 ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatc
     9841 tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat
     9901 cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt
     9961 tgttgctatt ttaaccagcc ttgccacctg ggtagttccg gtggggatgt ttgacagtca
    10021 ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc
    10081 atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac
    10141 gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaaggattaa cctcaggatc
    10201 gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg
    10261 cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg
    10321 cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt
    10381 atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg
    10441 gctgggctat gacagtatta ccaccgtcct ggtgacctat attgccacgc aaatcggttt
    10501 tgccagttcg tggatgaacc cgttttgtgt ggtcgttgct caggggattg ccggcgttcc
    10561 ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct
    10621 gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca
    10681 tgagtccgac cgcttctttc gtgaaaagca ggctgatgtt gaacaacgtc cgtttacctt
    10741 tggtgactgg ctggtattga ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt
    10801 gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt
    10861 gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc
    10921 atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa
    10981 agggattttg ctgctggtcg gtaatggtga agcgggtgat gccagcgtgt taaataccat
    11041 cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat
    11101 gttgctcttc caggcggtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt
    11161 aaccatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgcc aggttaccgt
    11221 gctggcattc cagtttggtg atggcttcag tcacatcatt tacccaacct cagcttcgtt
    11281 aatggcaact ctcggtgtat gcagggtgga cttccgtaac tggctgaagg tgggcgcgac
    11341 cctacttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg
    11401 ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt
    11461 ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc
    11521 tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact
    11581 gacttcatct tcctgtaggg cgaagggacc gtgagagacg cagctgaaca acgcgcccca
    11641 gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc
    11701 aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg
    11761 aaccacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg
    11821 acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg
    11881 cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt
    11941 gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc
    12001 tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac
    12061 ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat
    12121 gccataactc gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa
    12181 aatctccccc cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt
    12241 ctggtttaaa gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg
    12301 ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca gcagcatttg
    12361 atccacttcg ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca cttcattaag
    12421 ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt
    12481 caacacgtcg ccaaggatca ccagccactg ggcaccgctt tgggcaaaca actccagaac
    12541 acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca
    12601 ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt
    12661 gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat
    12721 aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg
    12781 ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc agtactaaca
    12841 aatgttctgc catcgcaaac agtttctctg cactggcttt tccctcggcc gttagtggcg
    12901 cttttttcgc ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat
    12961 cgctgcgcag ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat
    13021 aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag
    13081 attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac
    13141 cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg
    13201 acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag aaaaagtggg
    13261 catctgacca aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg
    13321 gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaattcgcc
    13381 gcgctttcta tacttgtggt gttcagatga ttaaacaacc ggagctgcaa tgatcgatct
    13441 ctatttcgcc ccgacaccca atggtcacaa aattacgctg tttctcgaag aagcagagct
    13501 tgattatcgc ttgattaagg tagacctggg gaaaggcggt cagtttcgtc cggaattttt
    13561 gcgcatttcg cccaacaaca aaattccggc aattgttgat cattctcctg ccgatggcgg
    13621 cgaaccgtta agcctctttg agtctggtgc cattttgttg tatctggctg agaaaacagg
    13681 actctttttg agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt tattctggca
    13741 ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca
    13801 aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtcttt accatgtact
    13861 gaacaagcgg ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat
    13921 tgcctgctgg ccgtgggtta atgcctggac tcgccagcga attgacctcg caatgtatcc
    13981 ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct
    14041 aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta
    14101 tttcatccta agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt
    14161 ataaagaacc ttcgtttacg tacgtttatc ggaattaagg aagaagaaat taacaaccgt
    14221 caggatattg ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa
    14281 gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag
    14341 aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa
    14401 catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc
    14461 gattcggtat ccatgacctt aagctggcag cgttaaccgc catatcggga ggctgcatga
    14521 acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattccg cgtttgctgg
    14581 agctgggcca tcaaatcacg gtagtgacgc gtaacccaca gaaagccagt tccgttctcg
    14641 gccctcgggt gacgctatgg caagggctag ccgatcaaag caacctcaac ggcgttgatg
    14701 cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac gagcaaaaag
    14761 agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcca
    14821 gcgacacgcc gccgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag
    14881 gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttacccat aaactctgcg
    14941 cccgctggga agaaattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc
    15001 gtaccggtgt agtgctggca ccggatggcg gtattctcgg taaaatgctg ccaccgtttc
    15061 gtcttggcct gggcgggccg attggttccg gtcggcagta tcttgcctgg attcatatcg
    15121 atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata
    15181 tggtttcgcc ctacccggta cgcaatgaac aatttgccca tgcgctcggt catgcgctgc
    15241 atcgcccggc cattttgcgc gtccccgcga ccgccattcg actgttaatg ggcgaatctt
    15301 cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg
    15361 cgtttcgctg gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac
    15421 agcaaacatc cgccagttaa ctcccggtgt tacaggatta gtggctttgc gcgataagat
    15481 cgtctggtga aagtcgggtc accatcataa ctaactctct gtctaaacct ctatccagca
    15541 tctcctgagc aatacgcagg gcttcttcgt gtttgccctg cattgcgcct tcttcacgta
    15601 atctgtcagc aatggtcatc agtttctcct tttcttgtgg tgcgcgttcc gctatctcac
    15661 caataaatgc acgaaaacgc tgggcatccc ctgtttgtaa tacgtaatta aacagggctt
    15721 ttagctgtct gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc
    15781 ccaacagatc gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcggt
    15841 gttgcataat ctcgtcatcc ggcaccacgg taatatccac caacggaaac gctgatgaat
    15901 atattttgtg ggctatagca ggctcggcaa attcatcaag ccagcagagt gaataaggat
    15961 aaggacttct gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc
    16021 ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta
    16081 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
    16141 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcgtcatca ataaaactgt
    16201 ttggttccag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa
    16261 tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
    16321 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
    16381 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
    16441 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
    16501 tccccatgaa aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc
    16561 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc
    16621 tcttcttcta ttttcccctg atggaggaaa atgacatgag tagaaacatg gcgagcaaag
    16681 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata
    16741 cgcaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
    16801 acttccggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
    16861 agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc cgacttttgc cagatacttg
    16921 accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
    16981 acgttttcca gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
    17041 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
    17101 cgcaccagat tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg
    17161 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt
    17221 acatctccgg cattcgcttg cagtgagacc cctttcagca cttcatgttc gccgtagcgt
    17281 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
    17341 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
    17401 agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc
    17461 ggcgttaata tcgcgggcta ttttcagcag atccggcacc gtggccgtaa acgccagcgc
    17521 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
    17581 agaaggcaaa ataatgcaac gatacatttt aaaagtcgag aagccatagg ctctggcggc
    17641 ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
    17701 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt
    17761 aaggaattcg gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa
    17821 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
    17881 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac
    17941 taccgacaaa ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt
    18001 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
    18061 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacagt ggtgaaaacc
    18121 aggtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc
    18181 agttgggtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
    18241 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
    18301 atgatccgcc gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgc
    18361 cctttcggca ctgccataaa cgcgccacgg aacgtttcgg taaaataagc accgtagata
    18421 aaaccgagag taataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
    18481 gcctccgtca ccgtgtttag cgcaatctgc aaaccgtaga aaatcagcag catcaacacc
    18541 aagtccggca cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
    18601 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagaa
    18661 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac
    18721 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
    18781 ccgaagggag cgggctttct ttcaccgatg gcgattaacc gccataaaca tcaaaatcga
    18841 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
    18901 tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
    18961 acagtttttc atctttaaca gacgggccac cgaatttgta atctttaccg acgggttgtt
    19021 tgaggaaacc ttcactggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
    19081 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
    19141 cccaatgttc attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
    19201 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc aaacgagaat
    19261 cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa
    19321 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
    19381 gattttcgac aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga
    19441 tatcgaagcc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
    19501 cggtaccgat gcggatgttt tgcggaatcg cagcaaacgc cgcagttgcg ctggagaagg
    19561 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
    19621 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
    19681 aaaatatccc gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc
    19741 aatgcaccac gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac
    19801 agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc
    19861 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt
    19921 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt
    19981 actgatgagc cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg
    20041 gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc
    20101 aaatcctggt tggcataggc caccacatcc acgcctttgc tacgccaggt ctcgttagcg
    20161 taagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
    20221 gttggctgaa tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg
    20281 tcggagaagg caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatggcg
    20341 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
    20401 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
    20461 tcacctttag catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc
    20521 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga
    20581 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
    20641 ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt
    20701 aacattcaac gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag
    20761 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
    20821 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
    20881 gcgggcaaag agatcttcag gaagggttat cgcaaactgg tcaagaacac gattaaccgt
    20941 ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat
    21001 aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg
    21061 tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg
    21121 ggtcagtaag ccatcagtat agctatggac aatgccggaa agggttttga ttgaacaggg
    21181 taaaatcacc atccctagcg tctggaaaga accggaagag atgctggcgg caatatcgcg
    21241 cgcatcgtgc gtgacatcgg ctaatgcctg cacttcgcgc agagaaaaat ccgtttcgag
    21301 ggataaggtc tggcgcgctg cctggctcat caccagatgc gtttcgatat ctgtgacatc
    21361 gcgcagaacc tgtaataagc gcacgccata aatcgcgccg ctggcgccgc tgatgcctac
    21421 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcaggatttc
    21481 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt
    21541 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac
    21601 gtaacgtatc gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt
    21661 tgaacaccga gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca
    21721 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa
    21781 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg
    21841 ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
    21901 ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga
    21961 cgtttttatc gcggaactcg gcgcggttgg cattcagttt acggcgcacg gacttacgac
    22021 gcagctgctg gcccggcatg ataaaggtgc ggccaacata gcggttttta acgaagccct
    22081 ggcggtacgg tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttctg
    22141 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca
    22201 gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgatg aacgagtccg
    22261 ggcgagcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt
    22321 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac
    22381 gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg
    22441 tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc
    22501 ggaaagcaac cataccgtgg ccgataatca tcgccacaca ggcatatgcg ccgcggatta
    22561 agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga
    22621 agttgtccag ctcgctagcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
    22681 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
    22741 cgttgtgggc aagcgtaatg ccatacggag agttaacgta aaacggctgc gcttcagagg
    22801 cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac
    22861 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga
    22921 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
    22981 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
    23041 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga
    23101 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga
    23161 tcagctgtga tttgctccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga
    23221 agagaatggc ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc
    23281 gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag
    23341 caccaacgat cagggtagcg ataaacagta ccgcgatggc aattccattt cgaaccagtt
    23401 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
    23461 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa
    23521 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
    23581 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt
    23641 gcgtagggat ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca
    23701 tcgacttttt atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt
    23761 atccgaccta cggttctaac actgcccagg cctgataaga cgcgccagcg tcgcatcagg
    23821 caagactgta ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct
    23881 tcaactcacc cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac
    23941 gggtaatttt accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc
    24001 gcagcttacc gacaatctca ttcactttat cggcattttt cagcgcaccc agttgcacaa
    24061 cataggcttt acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg
    24121 gcgcaggtgg tgcttcaacc ttcggtttag gcggctccac cggtttcggc ttcggtgggg
    24181 cgaccggtgc aggttccggt tcaaactcgg tgttattggc tgcaatagtg gtcggatcga
    24241 gcgacggtgc tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg
    24301 tcggcaacgc ctgggtggcg gctggcatca tatcaggctc atcgcgatcg cccgctttcg
    24361 gcaccagcgg gatagccgcg aactcatcct gataatgttt tttctgcccg tccagcagcc
    24421 ctggaagtac aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa
    24481 acttacttgc caccgcttct cctcgcgtca atcacttcca tgacatgtgc gacagtgtgg
    24541 aaagaaccac acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat
    24601 gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc
    24661 gtggcaccgc gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac
    24721 caggccagag ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc
    24781 ccgtttttcg gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc
    24841 gcgacatcaa aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt
    24901 gcgctggcaa tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt
    24961 gccgccagcg ccgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagc
    25021 gtgccgtgag catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg
    25081 ccccgacgtt gtaacagtgc acctttttcc tgtgccacat cagcaatggt agaaggcatt
    25141 tccggctcac cgacaattgc cggtttttcg ctgcggaaga tgcctgcttt ctcgcgacca
    25201 atactttcgc gatctggacc cagccagtcg gtatgatcca gcgcaatact ggttactacc
    25261 gcgacatcgg catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga
    25321 atcaccacgt caagttgtgc ctgcttgaac agccacaacg ccgacagcgt accgtactcg
    25381 aaataggtca gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg
    25441 gccgattccg gcaattcctg gccctgcaca cgtacgcgct cggtataacg caccagatgc
    25501 ggtgaactgt agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg
    25561 caggtggtgc ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcaga
    25621 acgccaagac gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat agttttactg
    25681 tgcaggtttt ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt
    25741 ttgataatca tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt
    25801 ggccctgccc ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact
    25861 acgccttcac gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg
    25921 ctcgccagtt tcaggcgcat ttccggacga cggacgatca tgtcgatggc gcctttctcg
    25981 atcaggaatt cactgcgctg gaatcctggc ggcagttttt cgcgaacggt ctgttcgata
    26041 acacgcggac cggcaaagcc aattaacgct ttcggttcag cgatgttgag atcgcccagc
    26101 atggcgaaac ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc
    26161 aagccgcgct cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac
    26221 atcagtgctt cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta
    26281 tcttccagcg cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa
    26341 ccgcccataa aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct
    26401 ttcatcacca ccagcgcatc tttttcgcca gtttctttct gcgcagatgc cagacggtct
    26461 ttatacttct tggagtcacg aaacttcagc acatctttcg gctcaagctc gctacccagc
    26521 tccacaaggc ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg
    26581 tgatggtcac acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc
    26641 tgaccgcagc tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga
    26701 gtaatgttgc ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacctgg
    26761 ttcgatgcca gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg
    26821 aatgttgcac attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg
    26881 gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt gtgacgaata aattcgaaaa
    26941 ccccaggcaa tattgaaaca aaaataatgc caacaatcat cagcttaagg ttgctctgaa
    27001 tgaaggggag cgtgccgaag aaataacccg cgtaggtaaa aatcagtacc cacaacagtg
    27061 cgccgatcac gttataagcg gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa
    27121 acggcgcgaa cgttctgacg atcggtacaa aacgggcgag aataatcgtt ttgccgccat
    27181 gtctctcgta aaactgatga gtcttatcaa gataactgcg acgaaagatt ttcgaatccg
    27241 ggttactgaa cagtttgtca ccaaataacc gtccaatggt atagttcact gcatcaccga
    27301 caatagcggc aatcaacata agcaccacca tcatatggac attgagatcg ttagtttcca
    27361 gcgacgccaa tgcaccagca acaaacagca gcgaatcacc aggcaggaat ggcgttacca
    27421 ccagccccgt ttcgcagaac agaatcaaaa acagaatggc ataaacccag acgccgtact
    27481 ccgcgaccag ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa
    27541 tcaggtccat atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc
    27601 tacagcctga aatgttccga gcattattct cgttagtccg ccagaaatag cgggcccatt
    27661 ggcggttttg gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc
    27721 gcttttgccg ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccag
    27781 ctctccggct ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata
    27841 tgatgtacaa aggcattcgc tttgatatct accaccacat aaggaccgtg acgcgtgaca
    27901 ttaatatgca taacgttacg ccacggggtt cgggactggc actgcaccgc acggaacgag
    27961 gtgaaatcat tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc
    28021 ggttcgtaaa aatgggttac ccctttactc agtaccgccg ggcgcagccg atgattgtag
    28081 atgatgtagc gataacggcg agccgtggcg ctaaatcggg catgaaaatc atcaggtaca
    28141 gctttaaccc aacgcacggc gatgtcacca ggtaaattcg catttacgcc caaggtccac
    28201 gccgcgtctt tgcgctgcgc ggttgtttcg aaatgcacca cttgcccggt accgtgtacc
    28261 cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt cgttcgccac ctgggagagc
    28321 gccttttcca gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac
    28381 ttactgccgt cgtactcaat gcccagcgca attttataaa ctggcagttg ttgctggtcg
    28441 gacattagta cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc
    28501 cgccaaagcg aacgttatcg gccaccgacc agaactggac ttgctccggc ataccgtagt
    28561 cattacgcac gcagccaaca gaaagatgcg gcgtacccga agcatcccct acctgagtcg
    28621 ggaattcgtt ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacgcgctt
    28681 cttctgccgc cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata
    28741 ccggtgcctg gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta
    28801 cttcgtcaac gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca
    28861 gcatattgaa cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga
    28921 gcaatttcgc actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg
    28981 aaatcaggct ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg
    29041 ccgccagcag ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat
    29101 cggtcagtac aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa
    29161 acagtccgct gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag
    29221 cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag
    29281 cggcatcctg cacggtgatt gtcttaccac caaagcgcag ttgttcgcct gcgctttcgt
    29341 tacgtgccag tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca
    29401 gggcttcgcc cacagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg
    29461 tggtttactc cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag
    29521 agattaacgt gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc
    29581 actggcgtca tcacaaatta catacagaga tgaccattca cggcgctcaa gatagttttt
    29641 gcgcagttta tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg
    29701 gcgcacatca tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcgggccatg
    29761 cagcgtaatg cgaccaaact ctggcgcggg cagtaatgta tccagcgcaa cgtgctgttc
    29821 atgcccaata aacttgctat aggcttcaaa cacttgcgta gtaccgcgtg ctttaccttc
    29881 cagggtataa cctgcgatat gcggcgtgcc gatatccact tttgtcagca actccacgtt
    29941 aagttccggt tcgccttccc agacatccag cactacgctt aacttctggc cttcattcag
    30001 gcaggtcagc aacgcagtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc
    30061 tcccggcttc aggctacgga tcagtttttc atccgccaga tgcagcgttt tgtacggccc
    30121 atctttgaag agtggcgtat ggaaagtcag aatatccgca tgctgaacta attcatccag
    30181 cgagcggaaa tcaccctcat ccccacggtc agcgcgaggc ggatcgcaaa gcaatgtttt
    30241 aatccctaac gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat
    30301 ccccaccgta cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gtagtgagga
    30361 gaaaacatat tccaccaccg caatcgcatt acagccaggt gcagcggaaa aaccaattcc
    30421 cgcctgcttc aaccatgctt catcgacatg gtcggtcccc gcagtggcag tgccaacaaa
    30481 tttaatgggt tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag
    30541 cgcatccgcg tctgccagtt gagcaacggg gattggacgc ccgggaaccg cggtcacctc
    30601 acccaaacgg ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac
    30661 gtttgtgtta cctgtatgag acgagagtta accggacaag tgtgccataa tctcgcggcc
    30721 aggcatactt gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc
    30781 ctcctcctgg gcaaccacca ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc
    30841 ctttatccag tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga
    30901 cgcaacaaca aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag
    30961 attcaccgct gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc
    31021 tggccgcgca aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac
    31081 gtctggggaa taatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc
    31141 cgctgaatca gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga
    31201 agatggttat tccgcaacca cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg
    31261 agcacaatgc gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca
    31321 aacagatctg gcaatcgatg ctggaacttt ccggggtgaa agatggcgag ttaattccag
    31381 cgaaactgtt taaccatctg gtgacctggt tgcaggcgcg ccagacgtta agccagcaaa
    31441 atacgccgac gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac
    31501 tggcggcgtt atcggcatat atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt
    31561 cttctgccca ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg
    31621 atccgcgtga tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa
    31681 atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct
    31741 ggctggttcg ttagccccga cgaaatgaca gtatcgtgac aataattccc agcaccgcag
    31801 agatcgcccc ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca
    31861 gcggcccgga gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc
    31921 cgcgaacttg tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg
    31981 aacatccggc tccggttaac gccgcgcccg ctaatgcgac ccacgcacct ggggcttgcc
    32041 agagcagcaa caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc
    32101 caaaacggtc cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg
    32161 cggtaagagt aaagcccgcc atcgcccatc ctttgctggc aaagtagagc gaaacgaaag
    32221 tcccgataac cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa
    32281 acccgacaac gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg
    32341 tgccgttaca ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa
    32401 aaccgtaatg gctatgaatc aacaggccaa gcggagcacc aacagcgagg gcaccgtaaa
    32461 tcgccattcc attccatgac atcactttgc cagagtgttt tggccctacg atgcctaacc
    32521 cccaggtcag agcgcctgtc agtaactggc tttcaccaaa cccaagaatc aaacgcccga
    32581 tgaccaacag ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg
    32641 cgccgccagc cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcaccatatt
    32701 gatcggccag tcgcccggca tagccacgcg tcagcaccgt agccagaaac tgaatcccga
    32761 cggcaatccc gaccatggta ttgccatagc ccagatcatg atgaacaaac agcgggataa
    32821 ccggcaacgg cagccctacg gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga
    32881 gcgaaaaatt ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca
    32941 gaatgcagcg caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacagtg
    33001 cgttggcagg atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg
    33061 cctggaactg gcggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc
    33121 gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc
    33181 gacctactcc gactactccg acctactgcg aattaatctt tcagcttgcg cattaccagc
    33241 gtggcgttgg tgccgccgaa gccgaagctg ttagacataa cggtagtcag ttcgcgatcg
    33301 gtcgtttcgg tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg
    33361 ctcggggcga taaagccgtg ttccagcatc agcagagagt agatagcttc ctgtacgcca
    33421 gcagcgccca gagagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg
    33481 ccgaacactt cacggatagc tgccagttct ttcacgtcgc caaccggagt cgaagtacca
    33541 tgagagttca ggtaatcgat tggggtatca acgccgtgca tcgccatctt catgcagcgt
    33601 actgcgcctt cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca
    33661 acgatttcag catagatgtg agcaccacgc gccagcgcgt gttccagctc ttcaaccact
    33721 accataccgc cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag
    33781 gctttttccg gggtgtcgtt gtatttagta gacagcgcac ccatcgcgtc gaattcgcag
    33841 gccatttccc agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt
    33901 tggatctgct ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg
    33961 gagtagttaa cgccatgaat tttaaacggc gtggcgaggc aggcagaaac gccggatgcc
    34021 atcgctttgg tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg
    34081 tcagcgccga acacctggaa acgcggggaa ccgccgccag aacctgcaat caggccaacg
    34141 cgcgggttat tctggtaagc ttccggagaa aggcccgcat ctgcgattgc ctgctccata
    34201 gaaaggaatg cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg
    34261 ccagtggtat ccagttttac gttgccccag acgtggctac gcatgccgga atccttcagc
    34321 tcctgagaga aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg
    34381 ttattaccga tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac
    34441 ctctgtaagt cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga
    34501 acaagtccga tcagccattt aatagagaaa tttgcgcagc cttacacaca tcgctaagat
    34561 cgagccaccg cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa
    34621 cctcgaattt aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc
    34681 caacgataac gggctggaag agacgcgtta tgtttttctg ggaggcaacc aattagaggt
    34741 acgctttcct gagcatccac atcctctgtt tgtggtagca gagagcggct tcggcaccgg
    34801 attaaacttc ctgacgctat ggcaggcatt tgatcagttt cgcgaagcgc atccgcaagc
    34861 gcaattacaa cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt
    34921 agccttagcg catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca
    34981 gtggccaatg cccttgcccg gttgccatcg tttattgctc gatgaaggcc gcgtgacgct
    35041 ggatttatgg tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca
    35101 aaaagtagat gcctggtttc tggacggctt tgcgccagcg aaaaacccgg atatgtggac
    35161 gcaaaatctg tttaacgcca tggcaaggtt ggcgcgtccg ggcggcacgc tggcgacatt
    35221 tacgtctgcc ggttttgtcc gccgcggttt gcaggacgcc ggattcacga tgcaaaaacg
    35281 taagggcttt gggcgcaaac gggaaatgct ttgcggggtg atggaacaga cattaccgct
    35341 cccctgctcc gcgccgtggt ttaaccgcac gggcagcagc aaacgggaag cggcgattat
    35401 cggcggtggt attgccagcg cgttgttgtc gctggcgcta ttacggcgcg gctggcaggt
    35461 aacgctttat tgcgcggatg aggcccccgc actgggtgct tccggcaatc gccagggggc
    35521 gctgtatccg ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt
    35581 tacttttgct cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg
    35641 cgtcacgcag ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat
    35701 ggatttaccc gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg
    35761 cgttgcgaca aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga
    35821 actgacccgt aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata
    35881 tcagttacag aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca
    35941 gcaagcaaca cacagcgtag tggtactggc gaacgggcat caaatcagcc gattcagcca
    36001 aacgtcgact ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc
    36061 ggaactggca gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc
    36121 ggcgaatcaa catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta
    36181 cagtgaggac gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca
    36241 gtgggcaaaa gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac
    36301 ccgcgatcat ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata
    36361 tgcgtcgttg gcggagcaga aagatgaggc ggtaagcgcg ccggtttttg acgatctctt
    36421 tatgtttgcg gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct
    36481 ggcggcgcag atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa
    36541 cccgaatcgg ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc
    36601 tgctctcgca gtgtttgccg gatgcggcgt gaacgcctta tccgacctac gtgtgacgtg
    36661 tagatactga taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact
    36721 tactgctgtg acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca
    36781 cgcggcgaca attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc
    36841 accgcctgag cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg
    36901 ccacgcaaat acccactggc aaacaactca tcatcactgg catggtcaac cataccgtcg
    36961 attaatgcca gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg
    37021 tggcgctggc tttagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc
    37081 gtgtgataat aattttgtag tgctttaatg aagcgtgccg ggcgttcagg aatgccgttt
    37141 gctaggtaat ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg
    37201 aagtcgcctt ccagattgtc gcaactgacg ttaaacggat atcccgccgc cacacagaac
    37261 aaccagtcga gtgcctgcgg cttcacttca acatcttcaa actggctttg cgtttgggca
    37321 tcacgcccat ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg
    37381 gcaatacacc agtgcgaaat ctcatgaatt gcgctggcat aaaagccgtg ggcaaagaca
    37441 attcggttat acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta
    37501 atcagacggg tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctca
    37561 tagtggtgtg tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc
    37621 tatcgtaaag taatttggca ctcatcaccg ccgagacgat aacaatcatc gggcggatca
    37681 gcttttgtcc tttgctcaac accagccgtg aacccatgcg cgcgccgagg aactgcccca
    37741 ccagcatcac aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac
    37801 cgccaatgtt tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga
    37861 atccgcacag cgtaacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc
    37921 cgtcataaaa accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc
    37981 gctggcgatc ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatca
    38041 ccagaatggg caaaatctgc cgtaagacat cagcctgaac gtactgtacc aacagtgcgc
    38101 cgctcattga gccgacaaag gtcatggcaa tattgagttt ctgatcgctt aaactaacca
    38161 ctttgcggcg aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat
    38221 tggttgccaa cgcgttagcg ggagacattc ccgctgccat caatgccgga atggtgagta
    38281 acccaccgcc accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa
    38341 ctcccagcaa cagcggggaa accataaaca ggctattaaa cgtttccatt agatcacgtg
    38401 ctcatccagt agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt
    38461 tgttgttccc ggttttggag gtgcaaacca gctttgcagt tctgccccgc aaccatcgcc
    38521 tggtggcggt aaaggttgat cttcacactc cagactatcg gcaggacaac gtaatcgtac
    38581 atgcatatgc gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt
    38641 gcccgcatca aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gtgtgacgtc
    38701 tttgtcctgg gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtcggaac
    38761 aacgtgttta ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga
    38821 ggtccagcga gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct
    38881 ggcatgaccg ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt
    38941 acccatgccc agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg
    39001 gtgaccgaaa tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg
    39061 caacgtgtca gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc
    39121 cggcacaggt tgggttattt tttgccacgg cgtcgctgcc aggctagcgc tactggcaag
    39181 cagagccagc agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt
    39241 cacatcggca ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat
    39301 cgcttctgcg atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat
    39361 catctcaact tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt
    39421 cggtttcagc gccatatggg caatgatttg ctgcccgctg ctgataccgc cgagaatgcc
    39481 gcccgcatgg ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg
    39541 cagcgccact acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat
    39601 cagcgcatgg gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa gtccggcggg
    39661 gacaccactg gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc
    39721 gcgcatcagt tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga acggattttg
    39781 ttcaaccagc gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaggcagcc
    39841 gcggatttca ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac
    39901 gcgcatggcg gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta
    39961 tttttgttcg taggtgtaat cagcatggcc tggacggaag acgtctttaa ttgcgctgta
    40021 atcctgagaa cgctggtcgg tattttcgat caacaagcca atgctggtgc cagtagtaac
    40081 gccttcaaaa acaccggaga gtattttgac ctgatccggc tcgcggcgct gggtggtata
    40141 gcgcgatgtc ccagggcgac gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg
    40201 aatgcctggc ggaacaccat cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa
    40261 ggtggttacg cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt
    40321 gtgtttgcgt gtttacttaa tctttataaa tcgcgaaatg ttctcgtgcg gcaataagct
    40381 gctctttggt gagcataaac actccatcgc cgccgttatc aaactccagc caggtgaacg
    40441 gaacatcggg atattgttcc ataagatgta ccatgctgtt gcccacttca caaatcaaca
    40501 cgccatcatc cgcaaggtaa tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc
    40561 cgtcggtgcc agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg
    40621 acatatcttc cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat tgcactttcg
    40681 gcaaatcacg gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt
    40741 cgatgttctg ttcggcaacc gccagcgcgt cgggagagat atctaccgcg tcgacttccg
    40801 cttccgggaa ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta
    40861 aaatatgctg cggttgcttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa
    40921 tcggtgagcg cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg
    40981 ctttgttggt caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa
    41041 caatacggtg tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca
    41101 gcggcaggta gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc
    41161 cgtgaccgta ccagatattt gccgcgctga agcggcttac cgaccagcgc aacatgtcct
    41221 gaatggtttg cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca
    41281 gggcatgatc gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat
    41341 tcacgcgcgg tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt
    41401 aaactgtagg aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga
    41461 ggaggaccag gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac
    41521 gattgtccac cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca
    41581 ggagcaggct gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga
    41641 aggtccggtg aaatatgttc gcccggatgt cagccatttt gaggcgaaga aactgcgccg
    41701 cggcgattat tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa
    41761 gcaggaactg ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt
    41821 gatgcatggg cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca
    41881 tccgcatgtg atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt
    41941 ggtgttgatt gaagtggaag agtggttgcc gccggagttg ccctgaatgc tggcagcaaa
    42001 tgccggagca gcgtaaacgc cttatccggc ctacaaatat gtgataattc aataaattgc
    42061 aattcacttc gtaggcctga taagacgcat cagagcaata ctacgaacta cagttcattc
    42121 aatacatact gaaaacgaca cgtcgaatca atcggttgcc cactactggt attcacattc
    42181 accacaatat cgtgtagtac tttatccgtt accggatgac gagttggcgt aaccgttacc
    42241 gttgatttat cacaaccgta agaggtaaag gttaacaatt ctgataccgc gcggcagtat
    42301 gctcctctgc catcattatc agaaaaatta ctgccatcac agagtctgcc gccttccatc
    42361 aagtaggtcg aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg taacgcctta
    42421 ttatggctga cataattttc actccaggag atatcgtatg aagcgaccgg tatcgttggg
    42481 catattgtgt tatgcactgt aggggccgta tagcttccaa atccgggtaa atctgtatag
    42541 gaataaaagg atgcgatcca agggcaacca taatcatcag atgcaacggt aattttaaac
    42601 gtcatatatg cacaacgaaa aattttcgta tctatgccag ggcaatttga gttattaatt
    42661 gtatcaaagt cagtcagtac caaagttgga gctgaacccg acccagacca ggaaataacc
    42721 cgcaacgtat cgcctttata cgcaaaagaa tcatatttcc catatgcaac ctcctttggc
    42781 gttatcaccg taacattgaa aacactgctc gctacagcac cggttacctt gtccatggca
    42841 taataaactc tcacactgtc aaaactcgca ctcataagtt tatctccagc ataactctta
    42901 gatgcgccga caacactcag aacgagcagc attaggctcg cgaaaattat ggtccatttt
    42961 ttcatttcag aaatactcaa aggttgccgt cactgtggca ctaaagtctc cggacgttac
    43021 atctcgcccg ctctttgcct gtaaccaggt attaaaattt aggctgttat ttccctgatt
    43081 cagcgggagc gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc
    43141 aataccaatc ccagcgccgg atgctgatga cgaggagtca aaggccagaa agccgggtag
    43201 agcactatct tctgttcctg tcaacgttac tttgacatta aacaatgtag aactatgaca
    43261 atctttcaat tgaaacacca atggcactcg ctctgactgc ccaaggttca ttaaatcgct
    43321 ggcggcaatg gcaggaaaat gtacttctgc caactggcta ccattaatca ctggcgtaca
    43381 cgatttactg attaaattgc cggtaaagtg caaattatct cctgctattg tggttccact
    43441 gcccagcaac atcaggctac taataaaaaa caacgtacgc tttttcttca ttggtaatct
    43501 acctgtaacg tggcccaggc ctcaaagtcc gcctctggca gaacgacacc actctttttt
    43561 actggcacag ccgttaacac cggtaaatcg gtttcattca ccacaagtgg cgtattgatc
    43621 gtaaaactct gccctgcctg tttgagctga atgcctaaac cagtgatatt gctgcttact
    43681 gcagcattat caaaatcgct tggtgtgcca ctccagctca aggtcatttc cattaatggg
    43741 tcgcgaaccg ccgtatcgca ggtaatggaa tacggcacgg tttgctcata attgctgcca
    43801 ttaatgtcat caataagcac atcctgaaaa gttatttcaa tggtttgagc gttgctaatc
    43861 gtgcagttag gtggctgcac taaataaccg tgaaaagaga cgtttgagag tgccatcgag
    43921 caaaagcccc acagcaaaat aagcgagatc cgtttcattg gtaatccacc acaattgtgg
    43981 cactggcgtt aaattcagct gccgctaacg ttgtaccact ctgtttcacc ggcacagctt
    44041 ccagaacggg aactcccgag gaaaaattaa atggcaacca tgagccagag gttaagtcga
    44101 gtattgtgtg atcactactt ttctgaaccc gaataccaaa cccaggaatc cccgtgctga
    44161 taactgtttc accgttaatc acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca
    44221 ttgccagatt gttacaaaca agcgagtagt caatgggacg tttataatca accccattga
    44281 tcttattaat gaatacatta ccaaactcaa ctgccgcacc atttaccgta caaggaggag
    44341 gggcatcgac aagcacccgt aaattcatac ttactgactt actcgctgca actgcaaagc
    44401 cgctaccaac acaacatatc agtaacccag ttaccgccca gacagaacaa gatcctttca
    44461 tactggctct cccggcctca gcctttgcgg ctctgctctt catcgacctg gcaactgtta
    44521 ccgttgcaag tgaaaaagag cggcatccga gcgccatagt cgttgacata ggtcagtacc
    44581 ggcacgctac ccatcttcac attcagcggt gcagaggttt tcggcgacat cactattggc
    44641 gagaaaccgg ctgccggatt gccatttttc tgtgtgctgg cattactgaa aatgacgtaa
    44701 tacggtgtcg ggttattgac ggtaaattcc tgaccgttgc gggttaaagt aaccttatat
    44761 tgccaggggt ttttcatgct gacgttctcc agcgccttcg gtcgccagaa caacttaata
    44821 cgcgtttgta gtgctatttg cagcgtgttg gctttgtttg attttggcgg gatctcacgc
    44881 acgttgaagt agaacaggct ttcacgatcg gcaggcaatt tgttgatatc cggcattccc
    44941 tgcactttta cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt
    45001 aacggtgaac tgatcttgtt acccttttca tcttcgatcc agctttgggc cagataaggc
    45061 agtttcggat cgttattgcg caacgtcacg ctgattgatt tgtcgctttc attaaatatc
    45121 aaacgggtac gatctggcgt tacagaagcc tgggcagaca aactggcagc gctaagcagt
    45181 aacgctagcg tcgtagcacc caatttggca gagcaaagta aatcagacat ggatcattcc
    45241 ttaatcacgt tattggttag ctgaaacaga aacaggggga ttggtgggtg tcacctgttg
    45301 cgtctgctcc tgaatgacag gctttatgtc atcaggaaca accggcgcga cgttgccttt
    45361 atgctggcat ggcagtaaca agccgttaaa cagatcggca ggcagcgggt ccggcaggtt
    45421 gatatcgcaa tgcgcaacac cactccagaa cacactcatg tgttcgccag gtttcacccc
    45481 agccagataa acattgccgt catcatcgac aaggcccact gtctgctcgt tatcattttt
    45541 tacttctgcg ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt
    45601 ttgaccactg atgacggcaa atttgcggta gccaatcgcc ccttcagtta gcgtggcttg
    45661 taccaccgac tgggttgctt cggcgttttc cggcagtttg ttgaggtcga tatacgcctg
    45721 attgcggtaa tagttattga cgtcagaaac gacggcttta ccaaacatat tggtataaac
    45781 agcagcaccg ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg
    45841 tgtcccgccc atattctggg tacggtgaag tgcgccaccg tgggcagtaa gcgttgcgcc
    45901 gccctgtaac gacaagcccg cagaggtgta ttgcccttca tggtagttcg cactgaggtc
    45961 aacctgcgcc agcgaaccat catggctgta atagccgtca acgctggtgt gtttgtcact
    46021 ggtgccgacg ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg
    46081 actgctgtcc gtcccactgc catagttacc gttatagcta acggtactgt tgtcgcccca
    46141 cggcatactg agcgaaatgt acatgccttt gtcggcctgg ttgtcatact cgtaacggta
    46201 gccagtcatc gagatgctga cattacgaat actgcccata ttgaaatagt gcgagagcat
    46261 gatgttgtag tttgtctgct cctcgcgatc ccagtaggta tggcgggtat agttgagata
    46321 aaccgaaaca cccgcatcgc ggaagttctg gttataagtg gcggtgtaca tctctttgtc
    46381 gttgcccgtg cgtaccattc cgctgtcact ggcatccaga tactcgctca tggtcataaa
    46441 gttctcttcc gagaagcgat atccagcaaa agtaacgcgg ctgttgagct ggtcaaagtc
    46501 tttggaataa ctcacacgga aggagttacc gtccagcgaa cctttgccat aagcggtgtc
    46561 tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga atgtagacaa
    46621 atcgcgaccg acgccaagcg ccgcagactg atagttttca tctcccagtg cgccgccata
    46681 taacgaccag ccgttagcga tcccccagga agcttcagca ccagaaaaaa attcaccctc
    46741 gacatggtgt ccccactctt gcggacggcc catcatgatc ttatagcgaa cctgacctgg
    46801 gcgagtgagg tatggcatcg aggcggtgct gatgtcatat tcctgcacct ggccgttctg
    46861 ttcttcaatg cgaatatgca acgtaccgga gacagaatca ccaagatcct gaatacgaaa
    46921 cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc atctggctga cggtcacttt
    46981 tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcagat tgggaggcaa
    47041 catttgatcg tcagtactga cactgccacc aacatagtta aaaccatcaa aaatatcgga
    47101 tctgaggtaa tcctcgccca gcgccagttt ggcttttaat gatggtaacg cccgccaggc
    47161 atagtagcga ctccactccc attttttttg agtttcatcg ccgctgaatt catcgtcatc
    47221 attactgcga gtatgttgat agttagtctg ccagtcagca cgcatacgcc acggccccag
    47281 gttaaccccg accgtcccgt tgccgctgat ctcgttacta tcatcaccgc cattttcttc
    47341 gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc ccggagatgc cgtcatccca
    47401 acgtgaaggc ggatcccagt cgggataagt atattcgagg taagcctgtg gcagtgaaat
    47461 gactaatgcg gactggctta aatcagcctt aatttccatg ccttccagtt gaccggattt
    47521 caggcattta gcatcgtggc tccattgcag attgttcgcc acgtcttctt ttaaaccaaa
    47581 ctgcgctacc agttccggtg tcagacaagc atagcttttg ctcgcgtcat cttcaccagc
    47641 ataccagtaa atatcgtact cttccgccaa tggctgttta tttagttgaa cctgtaaatt
    47701 gtattttccg ggctcaacat atccctggct ggaaaaacgc ttcagatcaa tttttgtgtc
    47761 gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg tcttcagccc agacactgct
    47821 ataactccct gacattgcca gcgcaatgca ccacggaaga atccgtaatc gaaaaagaga
    47881 atggtcaggc atacacgttt ctatccgttt ttattcttgt tcggtcaaaa tccatttaaa
    47941 tcccccatag cagtgtcgct atgggggtat gccttacagg taagtaatct ggaaggtggt
    48001 atttgcttcg aaattaccca gatctggcgc atcagctgcg ccgaccagcc aggctttaaa
    48061 gttcagtgtc tgcttcgctt tgcctttatt ggtagaagtg tctttcacta ttttctggtc
    48121 aatacccatg ccgcttttgt aagaggtacc cagagagtca ccgatcgcca ggctaacatt
    48181 gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa taattgccgc tatttgcaga
    48241 agaaacggta ccagtaaagg tagtggtcat tgtttcctga gtatcaaata cgcaatcttg
    48301 cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta tttttcagtt tttcggcacc
    48361 gatatcaccc atatcaactt ccagtttgtg atcgtccgga acgatagaac acactgagtc
    48421 ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc gcagcgattg ttgccgtaga
    48481 ggtgaaaacg cccatcacca ttgccgcagc aatagctgtt ttaacaaact tactcattat
    48541 ttgtccttaa actataaata attaaaaata taaacattac aatacattga ttcagtcaat
    48601 acccaatgtt tatacccagc cattgaatac ggcatgcgat ccaaaaagaa caggtcgtag
    48661 aaaattgtat tctttggtgt ggggccgcat agtgatgttt ccccccccct gatgcaattt
//
LOCUS       DAZFYC010000020        47179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFYC010000020 DAZFYC010000000
VERSION     DAZFYC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.020"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..877
                     /locus_tag="ACHKW5_001349"
     CDS             <1..877
                     /locus_tag="ACHKW5_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7510778.1"
                     /translation="LTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            877..1365
                     /gene="ygfS"
                     /locus_tag="ACHKW5_001350"
     CDS             877..1365
                     /gene="ygfS"
                     /locus_tag="ACHKW5_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7510779.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(1401..2768)
                     /gene="ghxQ"
                     /locus_tag="ACHKW5_001351"
                     /gene_synonym="ygfQ"
     CDS             complement(1401..2768)
                     /gene="ghxQ"
                     /locus_tag="ACHKW5_001351"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO7510780.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(2804..4120)
                     /gene="guaD"
                     /locus_tag="ACHKW5_001352"
     CDS             complement(2804..4120)
                     /gene="guaD"
                     /locus_tag="ACHKW5_001352"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO7510781.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITLRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(4138..5538)
                     /gene="xanQ"
                     /locus_tag="ACHKW5_001353"
     CDS             complement(4138..5538)
                     /gene="xanQ"
                     /locus_tag="ACHKW5_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO7510782.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(5703..8573)
                     /gene="xdhD"
                     /locus_tag="ACHKW5_001354"
     CDS             complement(5703..8573)
                     /gene="xdhD"
                     /locus_tag="ACHKW5_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO7510783.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(8570..9349)
                     /gene="ygfM"
                     /locus_tag="ACHKW5_001355"
     CDS             complement(8570..9349)
                     /gene="ygfM"
                     /locus_tag="ACHKW5_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO7510784.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(9400..10728)
                     /gene="ssnA"
                     /locus_tag="ACHKW5_001356"
     CDS             complement(9400..10728)
                     /gene="ssnA"
                     /locus_tag="ACHKW5_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO7510785.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLHEAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(10731..13829)
                     /gene="ygfK"
                     /locus_tag="ACHKW5_001357"
     CDS             complement(10731..13829)
                     /gene="ygfK"
                     /locus_tag="ACHKW5_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO7510786.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(14151..14729)
                     /gene="mocA"
                     /locus_tag="ACHKW5_001358"
     CDS             complement(14151..14729)
                     /gene="mocA"
                     /locus_tag="ACHKW5_001358"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO7510787.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            14832..15602
                     /gene="yqeC"
                     /locus_tag="ACHKW5_001359"
     CDS             14832..15602
                     /gene="yqeC"
                     /locus_tag="ACHKW5_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO7510788.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            15650..17275
                     /gene="yqeB"
                     /locus_tag="ACHKW5_001360"
     CDS             15650..17275
                     /gene="yqeB"
                     /locus_tag="ACHKW5_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO7510789.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(17316..18248)
                     /gene="arcC"
                     /locus_tag="ACHKW5_001361"
     CDS             complement(17316..18248)
                     /gene="arcC"
                     /locus_tag="ACHKW5_001361"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7510790.1"
                     /translation="MSKKIVLALGGNALGDELAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(18296..19681)
                     /gene="hyuA"
                     /locus_tag="ACHKW5_001362"
     CDS             complement(18296..19681)
                     /gene="hyuA"
                     /locus_tag="ACHKW5_001362"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO7510791.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(19734..20945)
                     /gene="ygeY"
                     /locus_tag="ACHKW5_001363"
     CDS             complement(19734..20945)
                     /gene="ygeY"
                     /locus_tag="ACHKW5_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO7510792.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(21003..22199)
                     /gene="dpaL"
                     /locus_tag="ACHKW5_001364"
     CDS             complement(21003..22199)
                     /gene="dpaL"
                     /locus_tag="ACHKW5_001364"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO7510793.1"
                     /translation="MSVFSLKIDIADNKFFNGETSSLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(22257..23443)
                     /gene="ygeW"
                     /locus_tag="ACHKW5_001365"
                     /pseudo
     CDS             complement(22257..23443)
                     /gene="ygeW"
                     /locus_tag="ACHKW5_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
     gene            23922..25694
                     /gene="ygeV"
                     /locus_tag="ACHKW5_001366"
     CDS             23922..25694
                     /gene="ygeV"
                     /locus_tag="ACHKW5_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO7510794.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQVEIVDENLCRVAGTG
                     AYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTPVI
                     LQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISKIF
                     ATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGH
                     MQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRV
                     MRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQL
                     LESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREI
                     LPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIE
                     LLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGEVI
                     DSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSKKQ
                     VADELGIGIATLYRKIKKYELLNT"
     gene            complement(25734..26213)
                     /gene="xdhC"
                     /locus_tag="ACHKW5_001367"
     CDS             complement(25734..26213)
                     /gene="xdhC"
                     /locus_tag="ACHKW5_001367"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO7510795.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(26210..27088)
                     /gene="xdhB"
                     /locus_tag="ACHKW5_001368"
     CDS             complement(26210..27088)
                     /gene="xdhB"
                     /locus_tag="ACHKW5_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO7510796.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(27099..29396)
                     /gene="xdhA"
                     /locus_tag="ACHKW5_001369"
     CDS             complement(27099..29396)
                     /gene="xdhA"
                     /locus_tag="ACHKW5_001369"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO7510797.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            29811..30566
                     /gene="actS"
                     /locus_tag="ACHKW5_001370"
     CDS             29811..30566
                     /gene="actS"
                     /locus_tag="ACHKW5_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO7510798.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            30645..30718
                     /locus_tag="ACHKW5_001371"
     tRNA            30645..30718
                     /locus_tag="ACHKW5_001371"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30677..30679,aa:Gly,seq:ccc)
     gene            30883..32097
                     /locus_tag="ACHKW5_001372"
     CDS             30883..32097
                     /locus_tag="ACHKW5_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7510799.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRQEMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            32196..32900
                     /locus_tag="ACHKW5_001373"
     CDS             32196..32900
                     /locus_tag="ACHKW5_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510800.1"
                     /translation="MPIVENIPDLSHWRTVQEFSITQAALLLAGIDPYDYPTLEHARD
                     YKHERWKMAWGLANGMVTAIRRGVLTPVWIEYYPLWEVQRASGTPRTEIPDRLKKISM
                     DETIITRHSLLEWVRNERVDYVRPPRSISDKEIKPSNQSVIIDAESRVINSEETVLLT
                     YGHKSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILR
                     PTGLQQVGRRKRKTNS"
     gene            33148..33345
                     /locus_tag="ACHKW5_001374"
     CDS             33148..33345
                     /locus_tag="ACHKW5_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7510801.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSVAF
                     IESEVDEWINQRIDASRNNAA"
     gene            33410..34129
                     /locus_tag="ACHKW5_001375"
     CDS             33410..34129
                     /locus_tag="ACHKW5_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7510802.1"
                     /translation="MKRPNMPVQGQGFIQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARTENRFAGLAVGERQSAGEQQLGLLIVMQKIEQAAFNEAIQNGMTPTEAVREVA
                     RRMETFASLMTSAPSLGVNHA"
     gene            34122..34370
                     /locus_tag="ACHKW5_001376"
                     /pseudo
     CDS             34122..34370
                     /locus_tag="ACHKW5_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            34524..35510
                     /locus_tag="ACHKW5_001377"
     CDS             34524..35510
                     /locus_tag="ACHKW5_001377"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO7510803.1"
                     /translation="MKHVKHYQKEALSNYRAGGPVRREYPHDAGMAGKTKATTKGSPV
                     EIKEIKRITNYAMSVAGGQGYQPGGRFLVAFNRRGYFSTKSSRANRVGLVSGSGLSNN
                     LPCRCAFSRCRSSMDNAISCNSSFVIVCTRKAVRCSTSYHSAGIFYGCNLRDDSGRFA
                     THCININNVMLLCAFKENAGIFRTNFSRNTSDHLSKKAAYTEAIPYKTGIGITTPQEL
                     EAIHDAPASFFVSTYTHILSMVGCMGPTSVGLVSSNASCGNPVQSTASELTTSGGGYN
                     PKVRGAFHMAVSARTSDFCRPYPSGGDSCLKPSASFPVLLTSAAIPLASRRT"
     gene            35435..35620
                     /locus_tag="ACHKW5_001378"
     CDS             35435..35620
                     /locus_tag="ACHKW5_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510804.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            35624..35833
                     /locus_tag="ACHKW5_001379"
     CDS             35624..35833
                     /locus_tag="ACHKW5_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510805.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            35953..36081
                     /locus_tag="ACHKW5_001380"
     CDS             35953..36081
                     /locus_tag="ACHKW5_001380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510806.1"
                     /translation="MIERNHEEIDDHDLSVIAGLIKQLSSNVAVWLMTEEEKRGEQ"
     gene            36138..36239
                     /locus_tag="ACHKW5_001381"
                     /pseudo
     CDS             36138..36239
                     /locus_tag="ACHKW5_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            36257..38080
                     /locus_tag="ACHKW5_001382"
     CDS             36257..38080
                     /locus_tag="ACHKW5_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO7510807.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWLEQDGKLA
                     GDNIPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKERGKGDDTTSLALNQMGAS
                     QRGEVLLAHYGGELAINPDSDTVHYYNGVIWSPVQDKELQRTMAQIFIDEDICYSQNA
                     IKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFTPPA
                     EGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGK
                     SVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAIT
                     GGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENER
                     DPMLPEKIEGELAVVIRHLLTRFSDQDEARLLLHEQQKSEEALAIKREGDSLVDFCGY
                     LMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFSKPVTLTRFGADMPGAMAE
                     YGREYMKRKTKHGLRSNVTLTEESEDWMPSCAPVKNEGDKN"
     gene            38367..38816
                     /locus_tag="ACHKW5_001383"
     CDS             38367..38816
                     /locus_tag="ACHKW5_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510808.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTLYADVTWLNDSLMLIHRDLFEREEFRRDIEERAEKIYEKYSANS
                     KRAARAITYHFMTLYDLKADDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            38813..39262
                     /locus_tag="ACHKW5_001384"
     CDS             38813..39262
                     /locus_tag="ACHKW5_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7510809.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTAMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALDRAKRHTGQQGNQPPAGGDDPFSDDIPF"
     gene            39338..39694
                     /locus_tag="ACHKW5_001385"
     CDS             39338..39694
                     /locus_tag="ACHKW5_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7510810.1"
                     /translation="MATHDEEAAKKRRRINRLAEYWPELFGAENIKPLKAGVIHDMLK
                     DAKARELAIGHGVLRGALTSYIHTTRYLKAIVAGGARYGLNGQPCGEVTEEDKSVASE
                     LLKRRLEQMKQKNSQQ"
     gene            39714..40007
                     /locus_tag="ACHKW5_001386"
     CDS             39714..40007
                     /locus_tag="ACHKW5_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7510811.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWSEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALS"
     gene            complement(40455..40841)
                     /locus_tag="ACHKW5_001387"
     CDS             complement(40455..40841)
                     /locus_tag="ACHKW5_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510812.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVSVEIGNPPFDVSGVAYLLYGMD
                     SHNNRHQIESTRRTLEAMVRDGLLERVNVYERRDSVGHDVRRKVARYGLPGHSVVARD
                     SEGRNGAIDGECIRINATDVVLVEGQ"
     gene            41038..41469
                     /locus_tag="ACHKW5_001388"
     CDS             41038..41469
                     /locus_tag="ACHKW5_001388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510813.1"
                     /translation="MKKIDSLRISKVAIISVSLLFLSACSTNNIENMPRDARVKFALM
                     RAYSDNKFDNSTVIGEVLGLSCARQLGKTLVTPLPGGSAIATSSEGDITSESDAIQDM
                     RYKAAMMGGDAIVNVVCKSGGVDWIHNCWSTVKCVGDVIKK"
     gene            42038..42283
                     /locus_tag="ACHKW5_001389"
     CDS             42038..42283
                     /locus_tag="ACHKW5_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510814.1"
                     /translation="MIEEQRERQHKQEATQPDSVTAKNYARVVAEVMRREGTELNGQD
                     CLVIRTRVLGMLAARRRQEQRQNAAPYQWHKPERLRR"
     gene            42442..42834
                     /locus_tag="ACHKW5_001390"
     CDS             42442..42834
                     /locus_tag="ACHKW5_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7510815.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(43665..44038)
                     /locus_tag="ACHKW5_001391"
                     /pseudo
     CDS             complement(43665..44038)
                     /locus_tag="ACHKW5_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44756..46366
                     /locus_tag="ACHKW5_001392"
     CDS             44756..46366
                     /locus_tag="ACHKW5_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO7510816.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            46379..46498
                     /locus_tag="ACHKW5_001393"
                     /pseudo
     CDS             46379..46498
                     /locus_tag="ACHKW5_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            46458..47179
                     /locus_tag="ACHKW5_001394"
                     /pseudo
     CDS             46458..47179
                     /locus_tag="ACHKW5_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT        12251 a        11202 c        12131 g        11595 t
ORIGIN      
        1 gctgactttt ggcattcctc ctttcaaact cgataaaacg gtattaagcc agcggcgaga
       61 gatattcacc gcaatgggaa tcgactttca tcttaactgt gaaattggcc gcgatattac
      121 ctttagcgat ttaacttctg aatatgatgc agttttcatc ggcgtgggga cttacgggat
      181 gatgcgagca gatctgccgc atgaagatgc gcccggtgtc attcaggctc taccgttcct
      241 gactgcccat acccgccagc tcatgggatt gccggagtct gaagagtatc cgctgacgga
      301 cgtggaaggt aagcgagtcg tggtattggg cggtggcgat acgacaatgg attgtttgcg
      361 gacttccatc cgcctcaatg ccgccagcgt gacctgcgcg tatcgtcgtg atgaagtcag
      421 tatgccgggc tcgcgcaaag aggtggttaa tgcgcgcgag gaaggtgtcg agtttcagtt
      481 caatgttcaa ccgcaatata tcgcttgtga cgaagatggg cgtttaactg cggtgggcct
      541 gattcgtacc gccatgggtg agccggggcc ggatggtcgc cgtcgtcctc gtccggtagc
      601 gggttcagag tttgaattgc ccgccgatgt tctgattatg gcctttggtt tccaggcgca
      661 tgccatgccg tggttgcagg gcagcggaat taaactcgat aaatggggcc tgattcaaac
      721 cggtgacgtc gggtatttac ctacccagac gcatctgaaa aaagtctttg ctggtggtga
      781 tgcagttcat ggcgcggatc tggttgtcac tgcaatggcc gcaggaaggc aggcggcgcg
      841 cgatatgtta actctgtttg atacgaaggc atcgtgatga aatcgttaat tatcgttaat
      901 ccggctgact gtattggctg ccgtacttgt gaagtagcct gtgtggtcgc tcacccttca
      961 gaacaggagt tgaatgccga tgtcttttta ccccggctga aggtacagcg actggacagc
     1021 atcagtgcgc cggtgatgtg ccatcagtgt gaaaacgccc cttgtgttgg cgcttgcccc
     1081 gtgggggcgc tgacgatggg cgagcaggtg gtgcagacca attctgcccg ttgtattggc
     1141 tgtcagagtt gcgtcagtgc gtgcccgttt ggaatgatca ccattcagtc gttgccgggg
     1201 gatacccgac aacaaattgt gaaatgcgat ctctgtgaac agcgggaaga ggggccagcc
     1261 tgtgttgaat cttgcccgac acaggcgctg cagttgctta ctgaaagaga actcaggcga
     1321 gtccgccagc agcgtattgt tgccagcggt gagaatcctc tgtgaaagaa atctccgcgc
     1381 tgaaaggcgc ggagaatcta ttagattgcc caaccacccg cgtaaaatgc gaccagtgcg
     1441 gcagtaatga tcaccgtacc aatattcaac ttttgccatt cgcgagcaaa gacacggcct
     1501 acgaccagtg tcacaaagcc cagcataata ccggtaacga tattacaagt cagaacgatg
     1561 aacacggcac acaccaggcc agccattgcg tcaataaaat cgttgaaatc cagcttcgag
     1621 acgttactta acatcagcaa acccacgtac atcagagcgg gtgcagtggc gtaaccaggg
     1681 atcaaaaatg acagcggtga taagaacaga atcagcagga ataacgcccc cactacggtt
     1741 gcggtcaacc ctgttttacc cccggcggcg gttcctgccg ctgattcgat ataaaccgct
     1801 gcgggcgctg cgcccaccag accggagaat attgaactta ctgagtcact ggtcagggct
     1861 ttgccgccat tgatgatctg gttgtcttta tccaacaaat tcgcctgacc ggcgacggca
     1921 cggatggtgc cagtggcgtc gaacactgcg gtcatcacca atgccagtac actcggaagt
     1981 acggttggct ggagtgcgcc cataatatcg aggctgaaaa tcagagactt accatcttcg
     2041 ccagtcagac ttggcatcgc caccagaccg tggtatttca ccgctgggtc aaagattaag
     2101 ccgataatcg aaattgcgat aatcaccaac aagatcccgc cgggtacgcg gcacttctcc
     2161 agaccgaaga tgaccgccag ccccagcaag ctcatcatca ccgggaagga ggtaaacgca
     2221 ccgagcgcca ctggcaagcc ttcaatcggg tttttgataa ccatacccac accgttagca
     2281 gcaatcagca gcagaaacag gccgataccg atacctgtac cgtgagcgat acccatcggt
     2341 aaattacgta agatccaggt acgcacaccg gttacggaaa tagcggtgaa gatgacgccc
     2401 atcagaaaga ccgcgcccag tgcgacagga acgctaattt gttgcccgag taccagactg
     2461 aatgcggtaa atgccgtcaa ggaaatcgca caaccaatcg ccattggcaa attggcccac
     2521 aaccccatca gcaacgagcc gaagcccgcg accagacagg tggcaacaaa cacagctgcg
     2581 ggaggaaaac ctgctttgcc cagcattccc ggaacgacga taacggaata aaccatggcc
     2641 agaaaggtcg ttaagccagc cagtacttcc tgacgaacgg tactgccacg agcggtaatt
     2701 ttaaaataat tatccagcgc gccctgtggc tttggtgcgt ccggtgtttg taggatgtct
     2761 ccagacataa tgatgtcctc acgaggatgt taaagtttta ttattagttg cgttcgtaca
     2821 ccagacgacc atcaacgtag gtgcggtaga tcgaacggtc atcgcccaac gtcatcatca
     2881 cgaacaattt gtcgactaaa gaaacagagt tgtcatagcg cagctgctgt agcggagtgg
     2941 cggtgggttc catcaccacg aaatcagcct ctttgccagg taaaaagttg ccaatcaaat
     3001 cgtcaaggcc cagagatttc gctccgccga gcgtggccag gtaaaacgct tcatatgccg
     3061 agaggcgata gccttgtaat tgcaatactt tgtaggcttc gttcagcgtt tgcagcatgt
     3121 tgaaagtggt tccggcaccg atatccgttc ccatgcccac tttaactttc ttctgccatg
     3181 cttttttcaa gttgaataag ccgctgccga ggtaaaggtt ggaggtcgga cagaaagcaa
     3241 tgctggattt ggtttcgctg agacgatccc actctttttc ttcgagatgg acgcagtgag
     3301 caaagacaca gtttttaccg gtcaggccgt actgatggta aacatccaga taaccatcat
     3361 ggtcaggata aagcgatttc acccaggcaa tttcatcttt gttttcacag agatgggtat
     3421 gtacccacgt atccggatat tcttctttca ggcgttgcgc catcgccatc tgttcaggag
     3481 atgaggtcgg ggcgaagcgt agcgtaatcg catatagcag acgaccattt ttgtgccagc
     3541 gttcaatcag ttctttgctt tggtgatagc tgctttcggc agtgtcgagc agataatccg
     3601 gtgcgttgcg gtccatcatc accttaccgg caatcatacg catattgata tgactggcgg
     3661 cttcaaacag cgcatcaaca gattgcggat gaacagtgcc aaacaccagc gccgtggtgg
     3721 ttccgttacg taaaagctgc ttgatgaaga atgccgacat ttcgcgggcg tactctaaat
     3781 cctcataacg acgttcagta gggaaggtgt gtttattcaa ccactccagc aattgttcac
     3841 cataggcccc caccatttca ctttgcggat aatggatatg tgtatcgaca aagcccggta
     3901 ctatcagttt gccgcgatag tcgcgcacgc gaatagtgtc aggaatttga tgctttccgt
     3961 cttcccattc gccaaaccat tccacttttc cctgtttaat gagtaataaa ccatcctcaa
     4021 taaaccgcag cgcagaggca atctcttccg ggttatcgac cgtacgggtg acatcaataa
     4081 aactgcctcg taccgctttc aacgtgtgtt ctcctgacat cattgactcc tttactgtta
     4141 atccatctct tccgctgagg taataccagg cagaacgttt tcctgtcggt agccaccagg
     4201 gaggataata tttaataaaa tcgcagttaa cccgccagca caaatagggt tttcaactag
     4261 tacataaata gaggctggca atattttaaa aatttcggga tcgtaggaga cgccaagtcc
     4321 taaacctaaa gaagtggcga caataagtgt ttctcggcgc tttaaaccgt tggtgatgat
     4381 tatgcgaatc ccagcgatgg caatcatgga aaacatcaac gtcattgcgc ctcccagaac
     4441 tgccgaggga atggtcgtga agaagccgcc aatcatcgga aacaaaccga ggataaccag
     4501 cattaccgcg atggttcgcc cgacataacg tgaagcgacg ccagtcatct gaataacccc
     4561 attattttgc gcaaacgtgg ttaagggtaa tgacccgaca gcggaggcga taacagaaac
     4621 cagaccgtct gccagcacgc cgcctttcag tcgggactga tactcttctc cctgaatggg
     4681 tcggcgggaa accattgccg tggcggtgat atcgccgaca gcttccagca cgctaagcag
     4741 ataaatcgtg ccaaccacca ggaactgatg gaagctaaaa ctaaagccgt atttgaacgg
     4801 atgcgggatg gtgattaacg gcaaattgcg catactgctg aaatccacca tgcccaggca
     4861 taacgatgcg atatagccga cacatagccc aatggcgatc cctcccatgc gtagcaacgg
     4921 actgcgacag cagttaaagc cgatcaccac aattaaaacc aataaaccaa cgccgagatg
     4981 ttcgtaattg ccgaatgtac cgctgctttt ggctgcaaat cctccaccaa aatcgataat
     5041 gccgacttta atcaggctta agccgatcat cagtaccaca ataccgctga cggtaggcgt
     5101 aataacccga cgtaaatagg gcaagataaa tgaagatccg acaaccagaa atgcgccaac
     5161 gaaggagacg ccgagaagcg acgacatgat taactcttcg tgaaaaccgt cgcttttcat
     5221 gctgctgccc agcgcaatca tgaccgtaac aaatgaaaaa ttgactgact gaattgagag
     5281 taggccagaa ccgacgatgc cgtagcggtt tacttgtaac caggtaccaa taccagaggc
     5341 gatcatcgcc atagaaacaa gataggcagt tgtttcagcg gaaagctgta aggccgcccc
     5401 cacgattaac gcgggggtta ccatcggaac gaaaattgcc aacagatggg taatggcacc
     5461 aacgagagcc tgatgaaagg gagggcgatc ctccagttca aatataaggt cagaacctgc
     5521 attgtttata tcagacatcc cttcactcct tttaaagtaa atttgatcat ctgaattagt
     5581 aaaataaatt cccacgaatt tggggcgcag gacgccttga gaacggctta tctcattttc
     5641 gcagtcacta tttagtcatc gtccaaacag agaaacaggc cgccgaagcg gccatttatt
     5701 atttatattt tttccagcgc agtgagtatt ttctccggtg tgaaatgcca ttcgcgtaac
     5761 cagatgccgc atgcatcgtg aattgcggta gcaatcgccg gagctgcgcc atttacaccg
     5821 atttccgaga tcgatttcgc cccgaacggg ccgactttat cgtcgctcgg caccagcaca
     5881 gcgcggaaat cacgcggaat gtcaccaatt ttcggtgcgc cgtaactgcg taaatcacgc
     5941 gttaacgggt gaccttcggc gtcataaatg atctcttcgc tcatactgtg gccgatagct
     6001 cgcagggtag caccgtagat ttgtcccaat gctaattccg ggttgaccgg tgtaccgcag
     6061 tccagcaagg cgtagaattt atccaggcgg atttcacccg tacgcgtgtt gacggcaact
     6121 tcagcgaagt ttgcgccata cgggaaggcg aaatcaggcg tgatataact gccagttccc
     6181 accagtgaac caaagccggt gccggtttcg cctttatgag caatatcccc gaagctgact
     6241 tcgcctttct tgccgcgcac gacgcccggc gttgccagtt gaacatctgc cactgcctca
     6301 cccaacattt gcgcgccgtg gaacagaatt ttctcccgca gattttccgc tgccaaacgc
     6361 gccgcgttac ccgagaagca agtaccggac gaggcatatg cgcctttatc aaacaacgca
     6421 tgatcggtat caccggagat aacatgcacg tcctgcggtg ggcagtgcag cacttctgct
     6481 gccagtttcg tcactacagt atccagacca gtaccaatat ccgcaccgcc agaatgaacg
     6541 ataaaggtgc cgtccgattc cagtttgatc atgcagttag cctgatcgat atccgggata
     6601 cccgatttct gcatgataat ggcgacgccg cgaccgatgt gccagtcact attttgcggt
     6661 ttcggtgaag accattggat catctcgcga ccctgacgca ggatctcttc cagtgcgcag
     6721 ctggcggcgg aaggaacgga ggtcggcgct ttaccttcac cgattgcacc gagaattttc
     6781 agctcttgcc cttcgtgtac ccggttacgt tcgataattt ccagttggtc gatctgtaac
     6841 tgttcagcca gttccgctaa tgccatggtg atagcgaagt tacctttcgg tgcgccataa
     6901 ccctgataag caccatttgg gcaaatgttg ctgtagtagg tggtgacctg gaaatcgacg
     6961 ttatcgcacg gatataacgg cagcgacagc gccggtccgt tacacggtac ggtgagtgag
     7021 tggttgccgt aagggccagt gttggcgcgg aaatccatct tcactgccgt caggcgacca
     7081 tcttttttcg ctcccagttt gacggtgact ttcgcgacgt gacgagaggt gttagcaata
     7141 aactcttctt cacgggtgta gcggaacagt accggacgcc cggtcacgca ggttgcccat
     7201 gcgcacactt cttccagcag gatgtcctgt ttggagccaa aaccgccgcc aactcgctct
     7261 ttaatgacat gaactttatg ctgtttcatg cctacgaggc gcgcgacctg gcggcgtaag
     7321 tgccatggta cctgggtgga ggcgtggata accagacgat cgccgtccat ccgggtaaag
     7381 cagatatgtg tttcagtcgg gcactgctgt gcctgcgttg agttataggt tcgctcaatg
     7441 atcacatcgg catcggcaaa gcctttgtcc atatcgccaa tatgaccatg aatactggcg
     7501 gcgatatttt tgcgagggcg agaaccgatc gggaagttga tgatcatatg ctcgccgcgc
     7561 tgggctgcat ggctgttatc gtcttccaga gtatctggcg caccagcaac atacaccacc
     7621 ggttcatcgt gcacgacagg cgcatcttcc gccattgctt cgtcgatcga cattaccggc
     7681 ttaagcactt catattcaac gtcgatgagc ttcaatgctt cgagcgcaat ttcttcactt
     7741 tctgcgacga ccgcagcaac acgatcgccg acgtgacgca ttttcttgcc gaacatacgg
     7801 cggtcaagcg gtgacggttc cggtgcgctc tgaccacccg gggtatagta gatatccggg
     7861 caattcaggt gagtaataac gtgaacgacg cccggtaagg cttcagcttt gctgacatcc
     7921 agatgagtaa tcagtgcgtg agcgtgtggg ctacgtaaca ttttaatgac gcaggcgtca
     7981 gccgttacgc ggtcttcaac atagcagggt ttcgcctgca ccattttcgc ggcatcagtt
     8041 ttaggataat gcttgccaat gacttcaagg tcgtcacgga aagtcggagc gatatcaatg
     8101 gtggcctgcg gattattttt acgtgcaacc gccagttcaa tgacctgata gtattgttgc
     8161 cagcctgcat cgcggctgaa caaaccagaa agcgcgtcgt cgatctcttc gcgggtaggt
     8221 gcggcgatgc gatcgagaag atcggtgata atcagagctg cagctggatc gttataacca
     8281 gactgcacca cgccaacatc aaccattgcc tgttgaacca gacttaactc gttccatttg
     8341 cccaaagatt ctgcggtacg aatatctgcc ttctctaact gtgcggcaat aagcaaggac
     8401 gcgttaacga tattaccgtt aaagattatt gcgtcagaac cggcaaaccc gaagccatca
     8461 tcactgttgc gtacagagtg cattcccatg ttaaacaaca gcttttgcac gttttcgcct
     8521 ggattaacgg ttagctcctg aggcgcgcca tttaaagtaa agtggatgat catacggctt
     8581 cctccccagc ctgttggcag tcggcataca ggtcggctac cagaactccc gtgatatagc
     8641 gtttataggc cacgctgccc cgcaaatctt cctgcgggaa aatggcgttg gcgacagcct
     8701 gttcaagtgc attgccttcc agattttgtt tttcgacatc atgcagacgc agtgctttac
     8761 tggcgacgcc atccagcgca attcgcatac cgtcgtggtc tgtcattgca acggctgccg
     8821 tcacgacggt taaacctgcc tgagaacggc taattttgcg ggtcgcacag gtgcgatacg
     8881 gatctttaat gataatttcg gttagcaggc gatcgcatgg gcaggccagg tagtcctcga
     8941 ttgacagcgt ttcgccgttg ccaaaaacca gttcagcatc cagtgccaac aggacgggaa
     9001 gcagcaccga ctcttcctgg cgggcggcga tttcaccacc aatggtcgac tgattacgaa
     9061 catggcgtga gtaaacaaaa ccgagggctt cacgcagcgc tgcaggaata aatcgcgcat
     9121 cacgcagtgg ctgcaagcga gacattgcgc caatccgcag tgcaccgtta tcccagtcaa
     9181 cccagtccag ttccagatcc tgtaaggaaa tggcaatctt tttatcggta cgggttggtg
     9241 tagcattgag tttgctgccc ccggcgaacc agacggcttc atcctggtag cggcgcttca
     9301 gttccagcgc ctgttcgaca gagtcgggcc tgaaaaattg ttcaatcatc ttctttcctt
     9361 atttaaccct cttccctgcg ggaagagggc atctgtcatt tatgccagcg catccatccg
     9421 acgccacata ctggcagcgg cttttctggc ttgtgcataa atggaatcgc aatcgaagtt
     9481 aaactgacgg tcttcataga ccatcacacc attgaccatc acgctgtgaa cgctgcctga
     9541 gcccataccg aaagcgatat gcccggcgat attgtctgcc agcagcggcg tcggtgagtt
     9601 gtaatcgcaa atggttaaat cagctttgta accggcttcc agaagtccaa atttcgcgcc
     9661 aaagttgcgg ctcatcagtt cgttaccgtt agtcagggct ttggcaaaac tgtcaggcca
     9721 cagcggacca cccgcatcgc gatgtttaaa gaaggcaaat ttcatctctt caaacatgtc
     9781 cgaaccaatg ccgtccgttc ccaacgccag attgcggatg tcgctaaggt gatggttgta
     9841 gccgacatgg ttgttcatgt ttgaacgggc gttatgcacc aggaacgcat cgcgctgatt
     9901 gagtagggcg atgtcatctt tcgacaagta cagcccatga gcgaccagcg ttttgctatc
     9961 gatgagatcg aattgcgcca gtcgtgccag caggtctttg ccgtaccagt ggtgactgta
    10021 ggaaacgtcg taaaggtctt ccgcagcgtg aatatgcaaa ccacggcctg tggctttcac
    10081 ggcttcatgt agcatctcca gaccggcatc tggcacggta aacggcgcgt gagcaccgat
    10141 atgtgcttcc accagatacg gctcggttgc tgctttcttc gcctcatcaa tctgacgggc
    10201 gaagcggatg ttttcttcta caccttcctg caactctttg atgccgttgt tacggtcagt
    10261 agtttcaaaa caggtcatcg cgcgcaggcc aacttttaaa aatgcgtcgc gcaatgtgga
    10321 gagcgacccg ccgatatacg ccggagaggc atggtgatcg ataaccgatg tacatccgct
    10381 cttaatcgct tccagggaac aaatcagccc gctgtaatag agcgactctt catcaagggc
    10441 gcgatcgagc cgccaccaga gatttttcag cgttgagatg aaatccgggc agggggcgat
    10501 gtttgccata attccgcggg aaagccccga gtaaaaatgg ttgtgcgagc agacaattcc
    10561 cggcatcaca atccgaccat gcatctcttt gtagctggca tcggggtagc gttgcgtcag
    10621 ggcatcgccg atagcgacaa tcacatcatt ttcgatagcg atatcaacgc cttcctgcac
    10681 tttcgccggg tgtaactgca ctgcggtgac attcttcaga atcaacatga ttacacctcc
    10741 acgcggccca gcagataatg atgatgctgg tggacatggc tgatgatgcg gcacatatcg
    10801 ttcagctccg gtggtacgtt gttgaactga ccttcgctgt cgatgtttaa cacccagctt
    10861 tggttattca gtcgtactcg tacccggcaa tcttccacaa ggaagcctgg gttgctgctg
    10921 ttatcaaagt cttgcgccag gctgaaaacg gtgattttgt ctttgtatgg tttaccgttc
    10981 cacggacaga actgagcgca gttgccgcat tcgttacagt aagcgtcgag gtgcagcgtc
    11041 tggaatctat tctggaagcc tgggaccgca atggagacgt tggcgcggtt cgggcagaca
    11101 tccacacact tgctgcaaac gtagttacat tcgaggcagc gagcggcttc ctgggcgaca
    11161 aacgcgtcac gatcgtcact gttcaccaga gtgatagaga tatcgccttt acgttgatag
    11221 atttccgccg gattgacgtt gttccagtat ttatcgttct ggtgggaacg gatattttcc
    11281 cgactaagga tggcatcggt cgcccgacgc gcggttccga cagcagcgac aatggaggat
    11341 ggtccgcgct gtacgtcgcc gatcataaag acgtcagtca gacgagtttc gccattgtgg
    11401 tcgacgtctg gccagccgtt tttgtccagc ggcacgccca tcgcattcag ggcttcagta
    11461 tcctgctgtt caccaatggc ggtgatcagg ctgtctacat gcagtgttac tgtttcgttg
    11521 gtttcaaccg gacggcgacg gcctttctca tcgggttcgc caagcgacat aacgcgcaag
    11581 gttaaggtgc catcagcatc gaaacgttcc ggattattca ggaaacggaa ctccacgccg
    11641 tcgtgcaacg cttcttcata ctcttcgcgc catgcgggca tctcttgcag tgaacgacgg
    11701 taaacgatcg ttgctttttc tacgcctgga acgcgtaacg ccgcacgagc gcagtccatt
    11761 gcggtgttac ccgccccgac aacgaccaca tgtttgccca gcttgagcgc tgtacccttg
    11821 ttgtattcac gcaggaaggg gagtgatttc caaacatttt ggttgtcacc cgccagtttc
    11881 acaccgctat ttttatcagt gccggtggca atcagaacat agtggaagcc ctgatttttt
    11941 aactgttcaa cggtcaaatc gggtgagcag ccatactcaa atttcacgcc gtgatcggca
    12001 acaaaatcga tatcgtgctg aattaactca gccggaattc ggaactgagg aatgatattt
    12061 ttcaccacgc cgcccgcatt ggcttcgcgt tcaaacagcg taaccggatg gcccgctctg
    12121 gcaaggaagt aacctgctgc cagacccgcc ggacctgcac caatcacggc aaccggatgg
    12181 cgtgaaccag aaccggctgg tttgtgccag cgttgcttat attcatccca acctttttcc
    12241 agcgcgactt ttttcagttc gcggatattc agcgcactgt cgtaatccag gcgggtacag
    12301 ttgtattggc actggtgatc gcaaatatga ccggtaatgg cgggcagagc gttgcgttgg
    12361 tagatgagtt ccagcgcgtc ggcatagcgg tgttcgccaa gcagacggat gtattccgga
    12421 atatcttgct tgatagcgca ggcagtaaca cagggggcaa cgtagcaatc ggtcagcggc
    12481 aggtcttctg ccacttcaat acgctcttct ggcttccagt gtttctgggt gtattccatg
    12541 gttaacgcat ctgctgccag tctgttcagt cgttcgacgt caacatggtc aagtccccag
    12601 gcgtcggagc cttccagctc gcgcatgcag gcacttaagc gcaaatagcc gccaggtttc
    12661 agcaggtcgg ttgccatagt aatagggcga atacctgtat caaaaatatc gcggatagtc
    12721 agctgactgg caccaccgga ataagaaatg ggcagtttgc cgtcaaaggc gcgagagaga
    12781 actgctgcaa cattgatgga gagcgggaac agcgcacggc ctgacatata catctcttca
    12841 ccaggcagtg cgcctttatt gttgatggtg ccgagagtgt tagtcagttt tacgccaaag
    12901 ccgagtgatt tttcttttgc cagtgccatc aggcgttcca gcatttccag tgcttgcgtc
    12961 agcttgaggt cgtgatcaaa tgactcttct tttaagccta tgtaaccgaa accgcagaca
    13021 tcgaggatct cacgaacacg cgcgtatccc agtaaggtcg ggttaagttt cacaaaggtg
    13081 ttgagccctt tttcttccag catgtagcgg caaatggctt cgatttcatg cggaggacag
    13141 ccgtgcatgg tggagagggt gacgccctgc accatactgg tggggatgcg agcgggtaag
    13201 gcttgcaagc tttcgcgttt ttcctgcaat ccgtggcggg ctaaaaatgc gtcatcctgg
    13261 agtaatttat tcagcgtatc gcgatattgc gcgaatttcg gatggtcaga tgcgtccatc
    13321 atattgtcga tgaactgttg cattggcggt tgcttaatac cttcgaggtt gtaaccgacg
    13381 ctcatattaa agatgaacga tttaccggaa tcagaaggct ggaacatcgc ttcgagaagg
    13441 tgcagggcaa accaggcttt gaggtattca tcccaggctt taagcagggt aaattcggta
    13501 gaccattcgg tgttaaagca ctcgtcttcg gcatcgatac agggcttttc cagctccagg
    13561 cggtcaagaa tttggacggt ttttagttcg atgaagcgtc cgccagtcag ccaggaagtg
    13621 acaatatttt gcgcgagctg cgtgtgcggg ccagcggcag ggccgacggg agtggcacag
    13681 gtttcaccga agacgctaac agttttacct tttacaggtg agtaaaactg ttgctcggga
    13741 ataccaaaga ttgagcgttg ttgttggtat tcatcaaata tgcgcgtcaa aagttcctca
    13801 aacggaatgg gacgcataat atcccccata accctctcct tagcttatac agatatcatt
    13861 gagtgaccgg tgttgagaaa tccggtggtt tagggggata gagcaacaat cgcaccactc
    13921 ttgccaggtt tggcgggaaa tataaaaaaa tgtgaggcgg ctcgaaactt aaaggctttt
    13981 tgtgaatgaa ctcacaggtg ccaaatgaga attaaaatca ctcctgaaaa ttattattgt
    14041 cagcccattt atcacttttt ctcttttctc aaaatcagtg attccttatc attatgataa
    14101 agagtgctgg agagattaaa tatttgaacg caatcgttta actacatttt ctaaatttta
    14161 gtataccttt tttgcgctgt aataaagtcg tccggagtat caatatctaa aattatttct
    14221 gcattttcta tctcgacgga ataatgagct cccataagga gtgcctgacg catattggtg
    14281 acattgggtc gttggattgc ctgcatcaag catggttttg acactaaaat cggatggccg
    14341 gggatgccat tatggagtgg cagtattgcg ccatcatttc gtaacgacca gatttttcta
    14401 aaaatatcga tggtgagggt tggcatgtca ccgtgggtga gaaaacaatg ttctgtttgc
    14461 actgcgggta ctgcggcctt tactgatgtt agtaaaccct gcgcataatc tgggttgtgg
    14521 ataatagtga tattgctctg gttcgcataa cgttcgtgca gttcattacc acgatagccg
    14581 gtgactaaaa taattcggct acaaaactgc aacgcatttt tgatacttgt atcaagaatt
    14641 gtcccctgtt gccagggtaa catcattttc cattgcccca ttcttgatga taatccggca
    14701 gcggtaatta tacagtcgat ggctgacata gtggttccat tatttatagg ggctaataac
    14761 gcctgataat tgcgttactt cctgatggat taagttgggg ataatttatc ccagagaggt
    14821 cataaagact cgtgaaaagt atagttgacc catcggcatt agtcattgat ttaggtgcgc
    14881 agaaacgtcc cactgtgatt tctgttgttg gtgctggggg gaaaaccagc ttgctttttt
    14941 ggctggcaga actgctccag gctagcggca ggcgtgtgtt aatcacgacg acgacacata
    15001 tgtttatgcc aacgtctcac tggcccgtgg ttttctgtcg tgatcccgcc atgcttcctc
    15061 atgcgtctct tacatccccc atttcatttt gttttcacag ctggaaagcg aaccagggaa
    15121 aagtgcaggg atttacgcca gaagcgattg atgcactggt gcaacgacca gagtgtgacg
    15181 taattctcat tgaagcagat ggctcgcgtg gaatgccgtt aaaagcgcct gatgagcacg
    15241 aaccttgcat acccaaaagc agttgctgcg tgattgctgt gatgggagga catactttgg
    15301 gcgcgaaagt gagcacagaa aatgtccatc gctggtcgca gtttgctgat attaccgggt
    15361 taacacctga tgcaaccttg caactgagcg atctcgttgc gctggttcgc caccctcagg
    15421 gggcgtttaa aaacgtaccg caaggttgtc ggcgagtctg gttcattaac cgtttttctc
    15481 aatgtgagaa tgcgattgca caaagcgagc tccttcaacc gctgcaacaa cacgacgtag
    15541 aggcaatctg gctgggcgat atacaagaac atcctgcaat cgcgcgcaga tttgtgaatt
    15601 agcggcccgg gtattccggg ttactgaaag gatatccatt gaggttggta tgaatatttt
    15661 cacagaggct gcaaaactcg aagagcaaaa ttgtccgttt gcgatggcgc aaattgttga
    15721 tagccgaggc tcgactcccc gccattctgc acaaatgtta gtgcgcgccg atggttctat
    15781 cgtcggtaca attggtggcg gaatggttga gcggaaggtg attgaagagt cacttcaggc
    15841 attgcaggaa cgtaagccgc gattattcca tggacgtatg gctcgtaacg gtgcggatgc
    15901 tgtcgggtca gattgtggag gtgcaatgtc agtatttatc agcgtccatg gtatgcgtcc
    15961 acgtctggtg ttgattggcg cggggcatgt caaccgggcg atagcccaga gtgcggcgct
    16021 attaggattt gatattgccg ttgccgatat ttatcgcgaa agcctcaatc ctgaactatt
    16081 ccctccatca accacgcttc tccatgctga gtcgtttggt gcagcagtgg aagcactgga
    16141 tattcgccct gataattttg tcctgattgc cacgaataat caggatcgtg aagccctcga
    16201 caaactcatt gaacagccca ttgcatggtt ggggttgctg gcaagtcgcc gcaaggttca
    16261 gcttttcctg cgtcaattgc gtgagaaagg cgtggctgaa gaacatattg cccgtttaca
    16321 tgcgcccgtt ggttacaaca taggtgcgga aacgccgcag gagatcgcca tcagcgtgtt
    16381 ggcagaaata ttacaggtga aaaataacgc gccgggtggg ctgatgatga aaccttctca
    16441 tccttccgga caccagctgg tggtgattcg cggtgcggga gatatcgcca gtggtgtggc
    16501 gctacgtctg tatcatgcgg gttttaaagt gatcatgctg gaagtggaaa aaccgacagt
    16561 gattcgttgt acggtggcgt ttgcccaggc cgtgttcgat ggcgaaatga cggtcgaagg
    16621 cgtcactgct cgcctggcaa ccagctctgc ggaagcgatg aaacttaccg aacgcggatt
    16681 catccctgtg atggtagatc ccgcctgttc attgcttgat gaactgaaac cgctttgcgt
    16741 ggtggacgct attctggcga aacagaattt gggaacgcgg gcagatatgg caccagtaac
    16801 aatcgcgctt gggccgggct ttactgcagg gaaggattgt catgcggtaa ttgaaacaaa
    16861 tcgcgggcac tggctcggtc aggtgattta ctctggttgt gcgcaggaga ataccggtgt
    16921 tcctggcaat attatggggc ataccacccg acgggtgatc cgtgctcctg ctgcaggcat
    16981 tatgcgatcc aacgtgaaat taggcgatct ggtgaaagag ggcgatgtga ttgcctggat
    17041 tggtgagcat gaaattaaag caccgttgac ggggatggtg cgtggcttgt tgaacgacgg
    17101 cttggcagtg gttggtggtt ttaaaattgg tgatatcgat cctcgtggtg aaaccgctga
    17161 tttctccagc gtttctgata aagcccgggc gattggcggc ggcgtacttg aggcgttaat
    17221 gatgttgatg catcagggcg tgaaagcgac aaaagaagtg ctggaagtgg cttaaatgaa
    17281 aatggccgga tggtattacc cacccggcct taaaattact gacaaatcac cgttccggtt
    17341 ttgccttcaa tcccttcttt cgctttgctc aatacggtaa tcagtgcttc acgacctgcg
    17401 cgagagcgag caaacgaggc ggcagcttcc actttaggca gcatagaacc tttcgcaaaa
    17461 tgaccttctt cgataaagcg ttcagcatca cttaatgaca ggcgatcgag ccactgttca
    17521 ttctctttac cgaagttaat ggccactttt tctaccgcag tcagaatgat cagcatatcg
    17581 gcatcaatca tttctgctaa acgggcgctg gcccagtctt tatcgataac cgcgctggca
    17641 ccacgcagat ggttaccttc acgaataaca ggaataccac caccgccaac ggtgatcacc
    17701 acctggcctg catctaccag agctttaacc gtttcttttt caataatatc aaccggtttt
    17761 ggcgaggcaa caacacggcg atagccgcga ccagcatctt ctttcagagt gtaaccctgt
    17821 tttgtcagtt gctctgcttc ttgctcggta aagaacgaac cgatcggctt ggtcgggttg
    17881 aggaatgccg gatcgttagc gtcgacttcg acctgagtaa ccagcgttgc taccggttta
    17941 ttaatgcccc gagaaagcag ttcttctctt aaggcgtttt gtagatcgta accaatataa
    18001 ccctggctta aggcaacgca gacagacatc ggcagcattg gcgagtgcgc ttcagttttt
    18061 gcagcagctt caaaggcctg gttaatcatg cccacctgcg gcccgttacc atgagtgacg
    18121 ataacttcat gtccttgggc aattaaatca acaattgcct gagaagtaat ttttaccgct
    18181 ttcatttgtc cggccagttc atcgcccagc gcattcccgc ccagggcgag aacaattttc
    18241 ttactcattt ttcctcactc atcattcttg attgtgtata gatccctgac agtggttaga
    18301 gcacgggagg gacaaacggt ttgcgtcgca ggaaacgacc tcgcccggct ttgcctgtaa
    18361 aggtgccgtc acagaaaatc gtttcaccac gggataaggt tctgacaatc gcgccctgac
    18421 aggtaaaacc ctcccatggc gagtagtcgg cgttgtcgtg gagatggcga tgctgaattt
    18481 gttggctctg acgtgggtcg ataatcacca cgtcgccatc ggaaccgggc gctaataatc
    18541 ctttttgcgg ccacaggcca aacagcctgg cgggcattgc gctggttaat tcaacaaagc
    18601 gttccggtgt tatacgtccc gtcatcacgc cactggaaaa cagtaactgc atgcggtttt
    18661 ccacaccggg taagccattt gggcagcgac tgaaatcgcc tttagaaatt tgcaggcgtt
    18721 gagccatcga gaaggtgcag tgatcggtcg ccaccacgtc aatcgcacca tcgctgatgc
    18781 cacaccacag tttgtcctgc tcgcgtacgt tacgcagcgg tgggctaaga atgaacttca
    18841 tgccgtcttc tgtatcgtaa ctgcgttcgt ccaacaggag atattgtggg caggtttcaa
    18901 cccagactgg ctggtgattc gcacgggcaa gacgcagata atccagacct aagccgttag
    18961 acaggtgcac gatatagagc ggggcgttac cggcaatttg tgccaggtta atcatgcggg
    19021 cgatggcttc cgcttcgcat tccagagggc gactcaaggc atgatagcgc ggcgcggtta
    19081 accctgcggc gataaattcc gcccgcttgc tggcgatagc cgcatcattt tccgggtgca
    19141 cggtggtcag cgcgccggat tcatgcagac ggcgtaatgc ctgcaaaacc tcgtcatcgt
    19201 tgagtttgta ttgataggtt aaatagagtt taaaactgct cagtccttcc tcgaccatca
    19261 tcgggatttc gtcgaggatt gcgtgattaa tgtgctggat cacaccgtga aagctgtaat
    19321 cgatgaccgc tttatgggcg gcataaccac gataaacctc cagttgatgg cgtaaccgac
    19381 agccgtttgg gccaaatccc atatggtcaa taatggttgt tgtaccgcca cacgcagctg
    19441 cgcgggtacc ggtaaaaaaa tcatcacaac tgcgcgcgat gccgacatca atattgaaat
    19501 gcgtatggac atccacgccg ccagggaaaa cgtaacagcc agtggcatca atttcttcat
    19561 acgggagctg cggcgaaata ttgttgccca actggcgaac aatcccgctt tcaatcagca
    19621 aatcctgttt ggcttgtcca tctgcgttaa cgacagtgcc gtttttgatc aatacgcgca
    19681 tagcaaactc caaaaaccct ccggcgaacc ggaggggaat aagtgaagag taattactct
    19741 gttgccagcc agcttaacgg gattgcagcg tacatcgcgg cacaggtcac caggtgagat
    19801 ttccaggttt tttcgttagg tgcatgcgct tcaggttctt tacccgggcc aaagccgatc
    19861 accggaatgc cgtgacggcc catgatagat acgccgttag ttgagaaggt ccacttatca
    19921 acaaccggcg ctttgccaaa cagcccttcg taggcattca ccagtgcttt aacggtgaag
    19981 tgatcttctt ccactttcca ggtcgggaag tagcattcgg ttgggtaaac caggccagtc
    20041 caggacggac ggtcgtagtt gtacatagaa acaaccgcgt tagctttctg tactgcaggc
    20101 agggcgcgga tttcgtccag cgcgccttcc caggtttcgc cccaggtcag acggcggtca
    20161 atagagacgg cgcagctatc tgctacagcg caacggcttg gggatgtgaa gaagatttca
    20221 gaaacggtga gggtgccttt gccgaggaat tcgtcataac ccagacgttg ggagagttct
    20281 tgtaattcgc caagaatcgg acccattttg aaaatggcgt tgtcaccgcg ttctggcgca
    20341 gaaccgtggc agctaacacc ctgaacatca atacgaattt ccatacgacc gcgctgacca
    20401 cggtatacct ggcagtcggt tggttcggta ctgaccacaa attccgggcg aatgccggat
    20461 tgttcaataa tgtactgcca gcacagaccg tcgcagtctt cttcctgcac agtaccggta
    20521 accagcaggg tatattcatc ttccagaccg aggtctttaa tgattttacc ggcataaacc
    20581 atagatgcca tgccgccttc ctggtcggaa gtaccgcgac caccgatcag ctcatcagtt
    20641 tccatgcctt cgtacggatc gaagtcccag tttttgatgt tgccaatgcc gacggtatcg
    20701 atatgagcgt ccattgccac cagacgcggg ccgtggccga tataaccgag aacgttgccc
    20761 atcgggtcga tttcaacttt atcgaagccg actttttcca tctcttcttt aatacgatgt
    20821 actacgcgtt tttcgtcgca gctttcactg ggaatagcaa ccatgtcgcg caggaagcga
    20881 gtcatgtccg cctggtaatc ttttgctttt tcaagaatca gtttgaatgg aatattctta
    20941 gccattattt ttctccagat tttttactcc ccgttttgca gggagtagtt ccaatagggt
    21001 gattaaggtg ctacagcgtg tttgccttcc cagacaactt cgcggtagtg cttcacgtcg
    21061 gtgtcgcctt cagtgctgat aaccagcacc acggcatctt tgttcagcgc cagtttttcc
    21121 atcaggcttt gacgttgcgg gtgataatga accgctgcga gaacgcccaa accgacagcg
    21181 ccggattcac cggagatgat gcgcgggtcg ttgccgtacg gattacccag cacgcgcata
    21241 cctaatgcgg caacgctgtc ctggcaggag atgaattggg tggcgcagtt acgtaggatt
    21301 tcccagccca gcgggttagg ttcgccacag gccaggcccg ccatgatggt ggccatatca
    21361 ccgccaacgt tgacgatgtc gcctttgacg ccggagcgat aaatacagtc agctttgtca
    21421 ggttcaacaa taatgctgtg cagattttgc gggctgtaga cgtcgaccag ataacccagc
    21481 acaccaccgg ccattgctcc gacaccggct tgcagcagaa cgtgcgtcgg ggttacgccc
    21541 atttcacgca tttgctcgac ggcttcatct gccagggttg cgtagccttg catgatccag
    21601 gttgggattt tggtgtaacc ttcccatgcc gtgtcctgta ccacttccca gccgtgctgc
    21661 tgcgcgtgtt gcatggtcag gcgaacggta tcgtcatagt tcatatccgt gacgatgcac
    21721 tcggcaccga ggttcagaat ggcgtcaacg cgttcctgag cagaaccttt cggcatgtaa
    21781 atcaccgcat tctgtccgag ttgctgtgct gcccacgcca caccgcgccc gtggttgccg
    21841 tcggtggtgg tcgcgaaagt cattttttcg ccgatggcat ttttcaggtg ctcaaatgac
    21901 agcgtttcga tatcaagatg atatttttca cacaataatt gagcgatggc gtacgcaccg
    21961 ccaagcattt tgaaggcgtt cagaccgaat cgttttgatt cgtctttgac gagaattttc
    22021 ttcacaccaa aaaggtttgc gagatcgtcc agcgcacaaa gcggtgttgg gcgataacca
    22081 gctattttct ggtggaactg gcgcgccagt ttggcctggc tttgcgaaaa gagcgatgat
    22141 gtttcgccgt tgaaaaattt gttatcggcg atatcaatct tcaatgagaa aacggacata
    22201 tcctatcctt ttggcctctg gaaatagagg gccgaccagc tgatcggccc gaggggttat
    22261 ttcacgcgtt cttgcgcttc tttcagcagt tgctcgagca gtgcacctgg tttggcgtat
    22321 ttacgggaca ggatcatcgc ggcgatgata taaggcttcc agctggcttc tttgtaggta
    22381 gcgatacggt atttttcgaa tacgccttca gtcacttccc cttctttgca ggatacgccg
    22441 ctgatatcag ctggcaggca gtgcatgtac agggcttcgc catcacgggt cagttccatc
    22501 atctcttcag tacaatgcca gtctttgtgt tgcgcgttct gtgccagaca ctgtttttcc
    22561 agtgctttta agccttcgtg atcgttagcg cgcagcaatt cggtacgctg ttccatcact
    22621 ttgtaaggtg cccatgactt cggataaacg atgtctgcat ctttgaatgc ttcttccatg
    22681 ctggtgacct gacggaagct accgccagat gctttagcat tgtttttcgc gacttctact
    22741 acatccggga tcaggtcgta gccttccgga tgggccaggg tgacatccat accgaagcga
    22801 gtcatcagac cgatgatgcc ttgtggtaca gagagcggtt tgccatagct tggagagtag
    22861 gcccaggtca tggcgatttt tttacctttc aggttttcca gtgaaccaaa gtgttcacgt
    22921 aaccacgcga ggtcagccat tgactgagtc gggtggtcaa tatcgcattg caggttcact
    22981 aaagccggac gctgtggcag tacaccctgc ttgtaaccgt catcaagtgc agcgccaact
    23041 tcacgcatat aggcgttgcc tgcgcccaga tacatatcgt cgcgaatacc aatagcgtcg
    23101 gcgcagaagg agatcatatt ggcggtttca cgcacggttc gccgtgagcg atttgtgatt
    23161 tgccttcgtc gagatcttgt tgtgcaaggc cgagcaggtt aagcgcggaa gcataagaga
    23221 agcgggtacg agtggagttg tcgcggaata cggaaatacc taatccacta ttaaagactt
    23281 tggttgagat gttttcagca cgcagtgctt ttaatgctgc ggcaacgtcc agtacttgtt
    23341 tcagttcatc tggcgtctgt tcccacgtta acaaaaaatc tttctcgtga aggtgagagg
    23401 tcagcgaatt gatatcctta atcagctcat taacagtttt catcataaag tcccggtagt
    23461 ggaatgacag gcaaagtggt ttatttcact gtgacttgcc aaagtgattg gtgttttcac
    23521 agctatacct gggactaaca ataagtgtgc cagaattatc cacggggctg taaaagacta
    23581 atttgacgga taatgtgtgg ggttgatcac aaattgatgc cagcttcggg ttgtttgtaa
    23641 aatacgaaaa cgattggctt ttcttatcgc gttatttatt tactgctaag tatcagaaat
    23701 aagataaagg gacaggctat ctctcttcag tttgataaac tcaccgccgt aattctcata
    23761 ttgatataga aatgtgatat ctcttccata atttcattag aaagtgataa aaatcttatg
    23821 tttttatcaa agccaatttc gcctgagtta atatcatcat gatctgtgaa ccatcaacgt
    23881 cttcaatgat cgacttttaa gtaagtctgg aaggtaaagc tatggagctt gctactacgc
    23941 agtcagtatt gatgcaaatt caaccgacaa ttcagcgttt tgccagaatg cttgccagcg
    24001 ttttgcaggt tgagatcgtt gatgaaaact tgtgtcgcgt cgccggaact ggcgcgtatg
    24061 ggaagtttct tggccgccag ttgagcggca actcacgcct gctccgccac gtcctggaaa
    24121 cgaaaactga aaaagttgtg acacagtctc gcttcgatcc cctttgcgaa ggttgcgata
    24181 gtaaagaaaa ttgccgcgaa aaagcatttc tgggtacgcc tgtcatttta caggatcgtt
    24241 gtgttggggt gataagtttg attgccgtta cccacgagca acaagagcat atcagtgata
    24301 atttacgcga attttctgat tatgttcgcc atatatccac catttttgtt tcgaaacttc
    24361 tggaggatca ggggccagga gataacatca gtaaaatatt cgcgaccatg atcgataata
    24421 tggatcaggg cgtattagtt gttgatgatg aaagtcgggt tcagtttgtt aatcagactg
    24481 ccttaaaaac acttggtgtt gtacaaaata atattattgg gaaacctatc cgtttcagac
    24541 cattaacatt tgagagtaat tttactcatg gacatatgca gcatattgtt tcgtgggacg
    24601 ataaaagtga attaatcatt ggtcaattgc ataacattca gggccgacaa ttatttttaa
    24661 tggcatttca ccaatcgcat accagttttt ctgtagcaaa tgcacctgat gaaccacata
    24721 ttgaacaatt ggttggcgag tgccgtgtta tgcggcaatt aaaacgactc attagccgta
    24781 ttgcacccag cccatccagc gttatggtgg ttggtgaaag cggcacgggt aaagaagtcg
    24841 tcgcccgagc aatccataag ttgagcggaa gacggaataa accctttatt gctatcaact
    24901 gtgccgcgat tccggagcag cttctggaaa gcgaactgtt cggttatgtt aaaggcgcat
    24961 ttactggcgc ttctgccaac ggtaaaacag ggttgattca ggcggcgaat acgggcacgc
    25021 tgtttctcga tgaaataggt gatatgccat taatgttgca ggctaaatta ctgcgcgcta
    25081 ttgaggcgcg tgaaattctg ccgattggtg ccagtagccc aatacaagtc gacattcgca
    25141 tcatttctgc aactaatcag aatttggccc agttcattgc cgaaggtaaa ttccgcgaag
    25201 atctcttcta ccgacttaat gttatcccga taactctgcc accgctgcgt gaacgtcagg
    25261 aagatattga actattggtg cattactttt tacatctgca tacccgtcgt ctgggatcgg
    25321 tttatcctgg cattgctccc gatgtcgtcg aaatattgcg taagcatcgt tggcccggaa
    25381 acctgcgcga gttaagcaat ttgatggaat atctggttaa cgtagttcct tcaggtgaag
    25441 ttatcgacag cacgctattg ccgccaaatc tgctgaataa tggcacaacg gagcaaagtg
    25501 atgtaacaga ggtcagtgag gcgcacctgt cactcgatga tgcgggcggc acggcgctgg
    25561 aggagatgga aaagcaaatg atccgcgagg cgctttcacg tcataacagc aagaagcaag
    25621 ttgctgatga actgggcatc ggcattgcta cgctctatcg caagattaag aaatatgagt
    25681 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
    25741 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
    25801 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
    25861 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgcgccg
    25921 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
    25981 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
    26041 ccgtcgacca gcaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
    26101 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
    26161 aaaggcatcc cgttaatggt gcattcgatg gtaattgttt cgctgtgatt cattgcaatt
    26221 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
    26281 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
    26341 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
    26401 catgttggca gcggatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
    26461 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
    26521 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
    26581 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
    26641 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
    26701 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
    26761 tagcgacgtt acggatctgc ggtccagcaa tggacgtggc cgcagcacat aacgccggga
    26821 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
    26881 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
    26941 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
    27001 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
    27061 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
    27121 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
    27181 atcttcaccg cgttacgaat agcagcggca acaggaatta ttggtggctc acccagtgac
    27241 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
    27301 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
    27361 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
    27421 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
    27481 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
    27541 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
    27601 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
    27661 ggtcgttcaa ccagcacgat atggcctttt atcagggtca gattcatcgc tgactgatgt
    27721 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
    27781 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
    27841 tcggtatctt gagttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
    27901 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
    27961 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
    28021 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
    28081 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
    28141 cactccggca accctgcgct gtaaatacgt ttgcccgtga gcggattagc atctccttcg
    28201 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
    28261 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
    28321 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
    28381 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
    28441 ccggtgttag acagaacatc cagactataa cctttcaatg ttccgtcgcg gttcacgccc
    28501 atttgcccgt caatggtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
    28561 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
    28621 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
    28681 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
    28741 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
    28801 ctttccatgt gacaatgttg aataacgggg gtctgatagt gcccctgtac ctggtagtcg
    28861 gcggcatcga ttgtttgttg gacattaccc gtcgacatcg tgctttgttt cagtaaattg
    28921 ccaccgttat ggattggtgc agcgtcttct gccagcgccg cttctggcgt ggtgataacg
    28981 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
    29041 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
    29101 cgatcggcgg tatcgcgctt gttttcgtca agtgtccagg catgcccagc agtagcaaat
    29161 ggaatatcag gcacatcttc ccaggtaaaa atcgccagta cgcccggcaa acttctggct
    29221 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgca
    29281 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
    29341 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
    29401 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
    29461 tacgccagaa acgacgataa gcgttagggg aggtaggaag tgtgatgacg ttaaaaaaac
    29521 acagatatga tacgaaacag gactctgaat aggggcagtg atacgtatca atatgagtgg
    29581 cgataaaatc aatgtgataa attgggatta ttacgcatca ggatgggaaa tagggtaaaa
    29641 agtcaggaag ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
    29701 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
    29761 gaggctggct gctatgataa ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
    29821 gacgcctgaa taaaaaatct ctgggtatcg tgatgttgtt atcggttgga ctgcttttgg
    29881 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
    29941 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagaac
    30001 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
    30061 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgaccaaaa
    30121 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
    30181 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
    30241 gcaataaagg gattgatatc tcagctccac ggggcacacc tatttacgcc gcgggtgcag
    30301 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
    30361 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
    30421 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
    30481 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
    30541 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
    30601 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
    30661 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccaaa
    30721 caaacatcta ccaacgtcta ctaaagtcta ccaatccccc gaatatcaag gcctccagcg
    30781 attttcctcg tctacaaccg tctacagtca tctacctaaa tctacacaca gaagtgtata
    30841 gtaatgcgta taggacaaaa tcgttttttg ggggctatac acatgttgct ttcagacctt
    30901 gctatccgtc gcgcgaagcc aaaagagaaa gcctatacac tcaatgacgg caacgggctt
    30961 tcgttactca ttgagccgaa cggttcgaag ggctggcgat tgcgatacag gtttgcaggc
    31021 aagcccaaaa tgttgtcgct gggtgtttat cctgaggtgt ccctttctga tgccaggacg
    31081 gcaagggatg aagccaaaag gcttatcgct ggtggagtta atccgagtga ggtcagaaag
    31141 gcccaaaagc gagaacaggc gaacaaatgc ggtaacacct ttgaggcgat cgcccgtgag
    31201 tggtatgaaa aacgcctgga cagatggtca caatcatatg ccgcagagat gattgatacg
    31261 tttgagaagg acgtattccc gtatatcggc agcaaaccga tagcagaaat aaaaccgatg
    31321 gaattgctcg cggttctctc tcgtcttaat gaccgtggcg caactgaaaa attgcgtaag
    31381 gttcgacagc gatgtggcga agtgttccgg tacgcaataa ttaccggacg cgctgaatat
    31441 aaccccgctc ctgatctggc aagcgcattt gcaccacaca agaaagagca ttacgcgtat
    31501 ctgaaagttg atgagctacc ggagtttttc cgcgttttta atacgtacac cggaagccag
    31561 atcgtcaagc tgggtatgcg attgttgatt cttacaggac tccgcccagg tgaattacgg
    31621 caggcagagt ggcaggagat tgattttgat aatcgtctgt gggaagtgcc gaaagaacga
    31681 atgaaaatgc gccgtcctca ttgcgtgcca ctttcagaac aggcgatcgc tattctggaa
    31741 cagttaaaac acatgacggg gcagtatccg tttattttcc ctggtcgaat acagcacagt
    31801 aagccgatga gtgaaatggc tatgaacgtg cttattcgtc gtattggcta tgcagggcgc
    31861 gttactggtc atgggttccg gcataccatg agtacgattt tgcatgagca gggttataac
    31921 tccgcgtgga tagaaacgca gctggcgcac gtcgataaaa actcgatccg tggcacatac
    31981 aatcacgcgc aatacctgga cggacggcag gagatgctcc agtggtacgc cgactatatg
    32041 gactcactgg aacagggcgg gaatgtggta catggcaagt ttggaaaatg tggatgactg
    32101 gttgagtata cagtagtaga ctttgagcga ctaaaaaggc tgatgcaaac cgaactggat
    32161 cggccttaac attagaccgc ttgaaggggg agaatatgcc aattgtggaa aacattcctg
    32221 acttatccca ctggcgaaca gtacaggagt tttctatcac ccaagccgct ttattgttgg
    32281 ccggaattga tccttatgat taccctactc ttgaacatgc cagagattat aaacatgaac
    32341 gttggaaaat ggcatggggg ttggctaatg gaatggttac tgcaattcgt cgtggtgtat
    32401 taactcctgt gtggattgaa tattatcctt tgtgggaagt acaaagagca tccggcaccc
    32461 cccgtacaga gataccagat cgtttgaaga aaatatccat ggacgaaaca attattaccc
    32521 gtcattcttt acttgaatgg gtaagaaatg agagggtcga ttatgtaaga cctcctcgct
    32581 ctatatcgga taaggagatt aagccatcta accaatcagt aataattgat gctgaatcgc
    32641 gcgtgattaa ttctgaagaa acagtattgc tgacttatgg tcataagagc gaaggtcttg
    32701 aatatgtaga agacgcaata agagaacttt gggcaacata tgatccagat aatcaacaaa
    32761 cagcccctac aagaaatgat gttgttgaat acctgaaagg caggggagcg gggcagaaca
    32821 tggccgaggc tgtaaacctg atactacgcc ccactggctt gcagcaagtt ggaagaagga
    32881 aaagaaaaac aaatagttaa atagtgccca cccttcgtaa aaaaggtggc actatttgct
    32941 caaaactctt ttgtttatgg cgagaaatca tagttaagcg tgcgttttct ttgcttattg
    33001 atactggaat ggtggcagca ttattaaaat aatagtgggc actattccat tgaaatagta
    33061 cccaccatta aaaatagtac ccacctataa atatgttttc cttttatgca tcattgtaga
    33121 cggttataga cgttacgagg attgaatatg tcaaataact tacttcgtct ttctgatgtt
    33181 atgcgccgta ctggttacgg tcgcgcttgg atttatcgcc tgatcagcct ggggcgcttc
    33241 cctaaacctg taaaaattgg ttcccgctcc gtggctttta ttgaaagtga agtcgacgaa
    33301 tggattaacc agcgtattga tgcttctcgc aacaacgccg cgtaataatc ttttgggagg
    33361 ggggtattct cccccagcac taaacctgat ccgacagcag gagttttcaa tgaaacgtcc
    33421 gaatatgccc gtacaggggc agggcttcat tcagcctgaa atcagccaga acgatatatt
    33481 gattcgccac aaagcagagt cgcgtattga ttcccgcgtt atttctgagc gagcaggaat
    33541 acaacacgaa agtatcattg cgacgattaa atctcatcaa tccagattgc gcgagcttgg
    33601 ctcactgcct cgccagtcag tcaaggaatt aacctatttg aaatcgggaa aatctggccg
    33661 caagcgcggt cgtcctgaaa ttagttattt gctgaatgag ctacagcttg actatctgtt
    33721 acgcgttatc cgtgggcgtg acgcagaacg tatcaaccag ttcaagctgg atgttacgaa
    33781 ggcattcgcg aagcatcgcg ccgctgaacc agtccgtcgc gaatatctgc caggctatca
    33841 cgaaagccgt gacgggctta aagcactggg cgcagaacgt caccattaca tcaaccttgc
    33901 ccgcactgaa aatcgcttcg ctgggctggc tgtaggcgag cgtcagagcg cgggagagca
    33961 acagttaggc ttactgatag tcatgcagaa aatcgagcag gcagcattta acgaggcgat
    34021 acagaacggc atgacaccga ccgaagcggt gcgggaagtt gctcgccgca tggagacgtt
    34081 cgccagcctg atgacgtcag cgccgtctct gggggtgaat catgcgtaat tatgatttcg
    34141 ccgcaattat tccggtatca cctggcatta ttggcagata tgaaacgggc gttgtcagcg
    34201 cacgcgcact gcataaagcg ctcggtgtag gccgtgattt caccacctgg atcaaagggc
    34261 gtattgagga gtacggattt aaaaaaggtg tggattttga ggttgtagaa aatttgtact
    34321 ttgattcccc gaaacggggg agccaaagtg taaatattga acagtttgat tctggctggc
    34381 acgtcgcctg ggagaacgtg aacatgcctg acatccgcaa cgctgccgcc ctgtacatcg
    34441 atactgcgga taagttcgca caggtgagca tcaaccatgc tgattacatg cgccatatcg
    34501 tgagccattt cgccgctgag cgcatgaaac acgttaagca ttatcagaag gaggctttat
    34561 caaattatcg cgctggtggc cctgtacggc gtgaatatcc gcatgatgca ggaatggcag
    34621 gcaaaacaaa ggcgaccacg aaagggtcgc cagtggagat aaaggaaatc aagcgcatca
    34681 ccaactatgc catgagtgta gctggtggac aaggctatca gccaggcggt cggtttttag
    34741 tggcttttaa ccgccgtggg tatttctcga caaaatcttc aagggcaaat cgtgttggcc
    34801 tggtgagtgg cagtggatta agtaacaacc tcccttgccg ttgtgctttt tcgcgctgtc
    34861 gctcaagtat ggataatgcg atttcctgta attcttcatt tgtcattgtc tgtacccgta
    34921 aagcggtgcg ctgtagtacc tcatatcaca gcgcaggtat tttttatggt tgtaatttgc
    34981 gtgatgattc aggtcgcttt gcgacacact gcataaacat aaataacgtg atgttattat
    35041 gtgctttcaa agaaaacgca ggtatttttc gcacaaactt ctctcgcaac acatccgacc
    35101 acttaagcaa aaaagcggca tatactgagg ctatccctta taaaacgggg atcgggatta
    35161 ccaccccgca ggagttagag gcgatacacg acgcgccagc gtcttttttt gtatcgacat
    35221 acacgcacat cttatcaatg gtgggctgta tggggccgac ttcggtcggg ctggtttcct
    35281 ctaacgccag ttgtggtaac cctgtacagt ccaccgccag cgagcttacc acctccggcg
    35341 gtgggtataa tcccaaagtc agaggagcct ttcacatggc tgtatccgca cgcacatctg
    35401 atttttgtcg cccgtatccg tcaggaggtg actcatgctt aaaaccttcc gcgtcttttc
    35461 ccgtgctgtt aacaagcgcg gctataccgt tggcatcacg cagaacgtga aggccgaaaa
    35521 cgtccaggct gcaatcagca cagtaattaa caaatcagct aaagccggtt tatccggcgt
    35581 aattatccac gcagtgacat gtattcagga tgaggagtaa ttcatgtgtg atttttccga
    35641 tatgactgtt gatgaagcag caaaaagcgc aatgcaggca gaacttattt gttctctgat
    35701 gatgggtaac acaggtgaaa tgactgaggg tgagattgag tcattgctcg ccctgattaa
    35761 gcagcttaca ggtaaggctg gtagctggct gattgcggca tcgggtgatg atgcaatatg
    35821 cgtcaatgac tgaatccttt gttgttctga atatcatgaa attaaggggg tgcagtaatg
    35881 caggaaataa cactggcaga agcctcgcta taggcgcagc aggtagaagt aatcagctgc
    35941 atgattgagc gcatgattga acgtaaccac gaagaaattg acgaccatga cctttctgtt
    36001 attgcgggac tgattaaaca actttccagc aatgtggctg tatggttaat gacggaggag
    36061 gaaaaacgcg gtgaacaata acggcgaaaa ataaacggga aataaaatta agacgacgcc
    36121 ttcaggagat taattaaatg aaatcaaaaa atactggctt aactgccagt agcaaaactc
    36181 accccgaatt tatgcgcggg gatatttacc gcaatgaata cggtagccac atgacgatga
    36241 tcagcagagg taagaaatga aaagcgcacc gaacttaaaa aaacagcctc gagggaaaaa
    36301 acacgccgat acggaagtga ttattttcgc tggcagtgat gcgtgggcgc acgcgaaaca
    36361 gtggctggag caggacggaa aacttgccgg agataatata ccgcctgtcg tgctggctga
    36421 tgagcaactt aaagatattg gcaatctgca aattgtaccc gacgggcgca aatcagcgcg
    36481 gatatacaag gccggacacc tcgatcaggt gatggtcaaa ggcataggtc agaagctggc
    36541 ggcggcaggc gtacaggacg cagactatta ccccgaaggt atgcaccata acgagcggca
    36601 gaactggcgt aactatctgg agactgagcg caaaaacatt tctgacgggc tggttattga
    36661 gctgcctgta aagaaaaaag agcggggaaa aggtgatgat accacatcac tggcgctaaa
    36721 ccagatgggg gcaagccagc gcggcgaagt tcttctggca cattatggcg gcgaactggc
    36781 aataaaccct gactctgaca cagttcacta ttacaacggg gttatatggt cccccgtgca
    36841 ggacaaagag ctacagcgca caatggcgca gatctttatt gatgaggata tctgttattc
    36901 acaaaacgcc attaaatcgg cggttgatac catgaagtta agtttacccg taatggggaa
    36961 taccgcccgt aacctgattg gattcagtaa cggggtattt gatacccgta caggtgattt
    37021 ccgtgaacac gataaaaatg actggctgtt aattgccagt gagttaccgt tcactccacc
    37081 agcagaaggt gaaacgctgg caacacatgc gccgaatttc tggaagtggt tacgccgttc
    37141 ggtggctgag aatgaccgca aggcagatcg cgttctggca gcgttattta tggtgctggc
    37201 gaaccggtac gactggcagt tattcattga ggtaacaggt cctggtggaa gcggtaaaag
    37261 tgttatggca gagatttgta ccatgctggc gggtaaggcc aacacggtat cggcaagcat
    37321 gaaggcactg gaagatgcaa gggaacgcgc gttagtggtt ggcttttcac tgattattct
    37381 gccggatatg acccgctacg cgggggatgg ggcagggata aaggccatta cgggtggtga
    37441 caaggtggca attgacccga aacacaaagc gccatactca acgcgtattc cggcggttgt
    37501 gctggcggtt aacaacagcg ccatgtcatt cagtgaccgc agcgggggga tctcacgtcg
    37561 tcgtgtgata ttcaattttt cggaggttgt accggaaaac gaacgcgatc cgatgctgcc
    37621 ggaaaaaata gaaggtgagc ttgctgtagt gattcgccat ttgcttacgc gcttttctga
    37681 tcaggacgaa gccagactcc tgttacatga gcagcagaaa tcagaggaag cccttgcgat
    37741 aaagcgtgag ggggattcgc tggtggactt ctgcggctat ctcatgtcgt cggtaatgtg
    37801 tgacggcctg ttagtcggta atgctgagat tattcctttc agtccacgcc gttatctcta
    37861 tcacgcctat ctggcttata tgagggcgca cgggttcagt aagcccgtaa cactgacgcg
    37921 ctttggtgcg gatatgccag gtgcaatggc ggaatatggc agggaatata tgaaacggaa
    37981 aacgaagcac ggattgcgct caaatgtgac gctgacagag gaatcggagg actggatgcc
    38041 gtcatgtgca ccagtgaaaa atgagggtga taaaaattaa gttatagaaa aactgttcac
    38101 cactgttcac cctgtaataa atgtatttag aatcaatatg ttaagtggtg agcagttttg
    38161 taaaactatt cacccaagtg ttcacctgtt caccctgtcg tttgattttt gaacagtaaa
    38221 gggtgaacag ggtgaacagt tggtgaacac ctttacaaaa agtgttcacc ccttaacttc
    38281 atgaatctaa aggtgaaata gcaaaaggtg aacagttgaa caccttttgg gtataaattt
    38341 ttatttattc ctctgagaaa aattttatga cagagaaaca cgccaaaaaa tcacattcac
    38401 acgctatcga tttaaccgaa ccggaatctg ttgtgaggtt cgtatcggta cgaggcagag
    38461 ccacgcttta tatacctgac gaacatctgc atcactgcga caaacataaa cttccgatcc
    38521 tgattgtctg gaagcgaacg ttatacgccg atgtgacatg gctgaatgat tcgctgatgt
    38581 tgatacaccg tgacctgttt gaacgtgaag agttccggcg ggatattgaa gagcgggcag
    38641 aaaaaatcta tgagaaatat tcagcaaaca gcaaaagagc agcacgggcg atcacttacc
    38701 atttcatgac gctgtatgac ctgaaagcgg atgatgcaga aaaagcggca tgtgatttat
    38761 tcgatatgac aatggggatt atccaggagt accggaacaa ggagcgcaga ccatgacagc
    38821 acagattgca gcttacgggc ggctggtgga cgacccgcag gtaaaacaga ccagcaaggg
    38881 aacagcgatg acgctggcgc gtatggcggt atcactgcca tgcagtcagg cacaggacgg
    38941 acaggcgacg ttatggctat cagtcctggc atttggtaag caggccgacg cgctggcgag
    39001 acatgaaaaa ggcgacctta tcagcgtggc gggtaacatg cagatcagcc agtggaccgg
    39061 acagaacggg gaaacacggc agggctggca ggttatcgct gacagcgtaa tcagtgcccg
    39121 ttcggcgcgt cctggcggga agcggggaca acaggggaag gcgaccgaag cgctggacag
    39181 agcaaagcga cataccggac agcagggaaa tcagccacca gcgggaggtg atgacccgtt
    39241 ctctgacgat attccgttct gaatgacagg agcacaacat gaaccagcag accacacaac
    39301 aggaccagac cacacgtaga cccacggagc gcccatcatg gcgacacatg acgaagaggc
    39361 agcgaaaaaa cgcagacgta taaaccgact ggctgagtac tggcctgaac tgtttggggc
    39421 agagaatata aaacccctta aggctggcgt gatacacgat atgttgaagg acgcgaaggc
    39481 gcgggaactg gcgattggtc atggcgtatt acgtggcgca ttaacctcat acatccacac
    39541 gacgcgatac ctgaaagcca ttgtcgctgg cggcgcacgg tatggactga acgggcaacc
    39601 ttgcggagaa gtcacggaag aagataaatc agtagccagc gaactactga agcggagact
    39661 ggagcagatg aagcagaaaa acagtcagca gtaatcagcc ggaggtaaag ccagtgataa
    39721 cggacagcgt ggcacagaaa cttgaggagc gcggtttatg gcgacgtgcg gcgacccgat
    39781 ggtctgaggt tcttcttcac gcagaaacag acagggaacg cgaggaagct gccagacgtc
    39841 gggcgatatg tatcattaaa tcccgtcgta tgcctgagca gtttgtcacc tttggcgatg
    39901 tgaaaaaggc cgcagacaga acgctgaaag agatggggat taatccacag gatgaatggc
    39961 gaaattacag cttcagtgat gcaggcgacg atttagcgct atcgtgatac gcatctcatc
    40021 gccgtatttg ctcaattatc gcgattcgct ataatcacca ccgaaaggcc gttgatagtg
    40081 ttctcctcgg gcaactggag cccgactgaa tgaagccagc cagcggcttt tcggcactca
    40141 ttccggtgta tctcccatac atcgggatga gtgaacgacg aaaacatcac ggtgatttcg
    40201 tcatgccgcg ataagaatgc gcctctttgg gcaattgaag ccctctgtac agcgggttat
    40261 cgcggcaaag ttgtgataaa aacgcgcctc tttgggcaac tggagcccac tgtaaagcgg
    40321 cttatcacag ctggcggtaa cgggcgctca tctccgggca actgaagccc tctgtacaga
    40381 gacccgttac ggctgaccct gaatgatttc tgccacgtgc aactggagca cccggagaaa
    40441 tctttcaggg ttttttattg cccctccacc agcaccacat ctgtagcatt gatgcgtata
    40501 cattcgccgt caattgcacc attccggccc tcactgtcac gcgcaacaac agaatgacca
    40561 ggcaggccat aacgcgccac ctttcgacga acatcatgac caacgctgtc acgccgctca
    40621 tacacattaa cccgctcaag aagaccatca cgcaccattg cttccagtgt gcgccttgtt
    40681 gattcgatct gatgccggtt gttgtgtgaa tccatgccat acagcaggta agccacaccg
    40741 gagacatcaa acggcgggtt gccaatttca acgctgaccc acgccagatt gtcaggagtg
    40801 aagtaactga ggatctcttt tttgtattgc gtcagtctca tagtgacctt tcttgttgta
    40861 ggatgaatcc ctcatacaat aagccataag gcaaattttg aaaggacaga atcggcgggg
    40921 tacagatgat acgtgatgac aaggctgaag cgctggaagc acaagggtta tgcgatgggt
    40981 tgcggcaata tgaggcaaat atgaatattc aaattgttgt gttgtgaggt acgcaagatg
    41041 aaaaaaattg attctttacg aataagtaaa gtagctatta tatcagtatc tttattattt
    41101 cttagcgcgt gttctacaaa taatattgag aatatgccga gagatgcgcg tgtaaaattt
    41161 gctctaatga gggcttattc agataacaag tttgataatt caacagttat cggcgaagtg
    41221 ttaggattga gttgcgccag acaacttgga aaaacattag taacaccatt gccaggggga
    41281 agcgctattg ctacatcctc tgagggcgac attacttctg agtcggatgc aatacaggac
    41341 atgcgatata aagccgcaat gatgggaggt gatgccatag tgaacgttgt atgtaaatca
    41401 ggtggtgttg actggattca taactgttgg tcaactgtta agtgcgttgg tgatgtaata
    41461 aaaaaataac agttgccata ctgaaggtat tgattaatac atggaaactg caattacgac
    41521 gtgtgattgc agcttaccgt tagctacccc tgttacaatt gccctcttcc atcacagcaa
    41581 aatgctaccc ccttcacagc cccccctcgg aagcccctcc gatggggtga aaaatccctg
    41641 aaaaaatgcg cagaaaattg cgcaaaaaac tgcgcaaatc gatcataaaa ccgatcacaa
    41701 aatcgatcat aaaaccgatc acacagacca gcagagaaaa gccaggtatg acgcggcttg
    41761 caggggggaa aggccaccgg aaaaagtgcg catttttctc aaaaacgaaa cgcaacagga
    41821 agcgcaaaac gaaaccgata cgcgatgatc gaaaaagcgc gcaaatttac cggattcggg
    41881 taagcaattt tgcctgattt gggcaaaata cggtgtcgcc gtaacagtga cattgacacc
    41941 aaaacagtga cacacaggag gcccgatgac cgaacgttca ccaacagaga taattcacgg
    42001 gattattgaa gagcaggagg aacgcgagtt aatcctcatg attgaagagc agagagaacg
    42061 ccagcacaaa caggaggcca cgcagccgga cagcgtgacc gctaaaaatt atgcgcgtgt
    42121 tgttgcggaa gtgatgcgcc gtgaaggcac tgaactgaac gggcaggact gcttggttat
    42181 ccgtaccagg gtgcttggta tgctggcggc aaggaggcgg caggagcagc gacagaacgc
    42241 ggccccgtac cagtggcata agccggagcg gttacggcgg taactcactg agattaaagc
    42301 aaagctcaaa tctgagatgg ctacatccct cacttctgag gactgttatt cacaaactac
    42361 gaaattttcg tagtttaact ttcatccagt gcatccctga attacgggta tataaccaca
    42421 gccattacga ggtgattgtt gatgcgtgat attcagcagg ttctggaacg ctggggagcg
    42481 tgggcggcaa ataatcgcga gaatgtgacc tggtcatccg ttgccgcagg atttaagggg
    42541 attattccgg caaagataac atcacgctca cagtgctgtg acgatgatgc gatggtgata
    42601 tgcgggtgca tagcccgtct ttaccggaac aatcgcgatc tgcatgactt gctggttgat
    42661 tattacgtgt tgggggagac gttcatggcg ctggcacgga aacatgggtg ctctgacacc
    42721 tgtataggta aacgccttca caaagcggag ggggttgttg aaggcatgtt gatgatgctg
    42781 gatgtgaagc tggagatgga tcggtatgtt gagcgcgatt cagtgacggc ataacggcat
    42841 aaaaaaaccg cagtcatgag gaaactgcgg tggagaaata cggcttgcca atcaatcatg
    42901 aaaaacgtaa tcgcctgata attatgccac gagacaaagg acggtgcacc ctgaatggtc
    42961 ataatgacca ctcagaccat cgaggcaacc ataacgaaca tcgttacagt tggagctttt
    43021 accccgttgg gggaaaaggt gcatacctga atcatcatta tggtgatcaa gggcatgtaa
    43081 agcccaccag cctgattaaa ggttaaccgg aaaaagccag gtatggaggg agtgattagc
    43141 cacgaacaag ccggacacca ccagcagaag gtattatgat tttcgtaata cctttagcaa
    43201 agcgtgaatc atcattaccg tagggatggt cataatgacc acaccttccc ccaccggaac
    43261 aaggaaggct ggcttacgaa aatttcgtaa ccctccccag cacaatgtat taaataatca
    43321 ctacaggcca ttgattaaac ttaattatcg cgaatcgcga gaattagccc cgccggaaca
    43381 aggaaaggga aattacgaaa atttcgtaaa atccccccac atctcccacg gttgcccgtt
    43441 aagtgcaagc gcacaggcac gacctcatta accagttacc agcgctcacg ctcaaacatc
    43501 cacagcaatg gggataaatc gcactctggc gcaatgttca ctatcggggc gagcattcac
    43561 cggaaaacgt catacaggct tacaggcggc ttaaaattcg atgggcggag gaccggatgg
    43621 gaatcgggag acctggacaa aaagtgcagg cctccatgac acggtcagga cagtttgtgt
    43681 ttaaccagaa tatttagcgc ctgtattggg gcttcgtgga gctcccgcag acattcaggg
    43741 tcagtaacat gttcctggta tcttgttttc tcaagtaaca agcggtgcat atgcagcata
    43801 cgggcaagag ccgcgcacac ctcctcaaat gcggtatccc gcttaccgtc actgtcagag
    43861 ccacaaaaca gcggtacaac ctccacgccg tcgctgtggg ccttagccag gcatcaacca
    43921 cggttaaatc atcggtgaag aaccacgcac tgttagcgct gaatcgcatt ttccaggctt
    43981 ccggtactgg ttgcggggag tatgaatatg ctggtggtat cttgtcgtga gtatccatag
    44041 gctatcgttc cgtcattctg ttattcacca cagcgctaaa aacgccatga aggagcagaa
    44101 taccgattgc ctcgtttgct gtatgaggtg cttaccacac attgggtaca cagatgaatc
    44161 aggcggaagt gtgattaccg gaaaaaatcc atttcagttt gagggaaaaa aaccgtagtg
    44221 cgataacact acggcatgcc accaaagaaa cacaacaaga ggtcaacaat gagtaatgga
    44281 catgaccaga cgattttatc atgccggaac aaaagttatg tgacacagtc ggataaaaac
    44341 aggtaaagcc caccagcttc ataggggata accggaaaaa ttccggtaaa tatttgcctg
    44401 agtaagtcat caagcgcctg ctaaatgtgt atagaaatgt gtataggatt gattttagaa
    44461 catgtggaaa tggctttatt actggtggca tggcttgttg ttctgttccc ttcgccgctc
    44521 caatttatct tcacctgatc aatctgccat ttttgctgta tctgattttc tatattatct
    44581 taattcaatc gcttcagact attcgcaatc gccgtaatac ttccccattt tgttgtaaat
    44641 aaaaagcgtt tacgcaatgc aatccttttg taacaaccct ggtaaaacac cttattttca
    44701 agttgttaaa taagtggtta tctacgcttt taacaaacga ttgatggaat attttatgaa
    44761 agggaaatct gcattaactc ttctgctcgc tggtatattt tcgtgcggca catgccaggc
    44821 gacaggggca gaagtgacga gtgaatctgt atttaacatt cttaattcca cgggggcagc
    44881 aacagataaa agttatttat cgttaaatcc agataagtat cctaattatc gtcttttgat
    44941 tcactcagcc aaattacaaa atgaaataaa gagccactat accaaagacg aaattcaagg
    45001 attgctaaca ttaacagaaa acacacgaaa gttaacgttg acggagaaac cctggggaac
    45061 gtttattctt gcttctactt tcgaagatga caaaacagcg gcagaaaccc actatgatgc
    45121 tgtctggctg agggatagtt tatggggcta tatggcatta gtatcagatc agggaaatag
    45181 tgtagccgca aaaaaggttc tgctcaccct gtgggattat atgtctacgc ccgaccagat
    45241 taagcggatg caggacgtca ttagcaaccc caaacgactg gatggagttc ccgggcaaat
    45301 gaatgttgtg catatccgat ttgacagcaa ctcccccgtg atggcagatg tgcaggaaga
    45361 aggcaagccg cagctgtgga accataaaca aaatgatgcg ttaggtcttt atcttgatct
    45421 ccttatacaa gcaatcgaca ctgatacaat taacgctgaa gattggcaaa aaggtgaccg
    45481 attaaaatcg gttgcgctgc tgattgctta tctggataaa gccaatttct atgttatgga
    45541 agattccgga gcatgggaag aagatgcccg attaaacaca tcttccgttg cactggtgac
    45601 ctctggactg gaacgtttgt cgaacttact ctcgaagaaa gactctgttt ttgtctctga
    45661 tttactacgc gaagctaaag ccaatgaact ggatgaacct ctttcaacaa cgaggttgaa
    45721 ccacctcata gataaaggtt atgaaagaat aacgttacag cttgatttag ggggagaatc
    45781 cccaggttat ctggaaaaag ataaacatta cagagaagcc gatgcggctt tacttaatgt
    45841 tatttatcct gcaaatcttg caaagataaa caccagaaga aaagagcagg ttctaaaaat
    45901 agttaaaaaa ctagcaggtc cgtatggtat aaaaagatac gagaaggata attatcagtc
    45961 ggcaaatttt tggttcaatg atattaaaac cgataccgat caaaattcac atgcgaaaag
    46021 agaaaagagc ttcattccat caacagaagc agaatggttt tttgactcat ggtatgcaaa
    46081 atcagcagca attgtttata aagaatcaag aaaggaagag tatttgaatg actcagtgca
    46141 atttatgaat agatcattgg cccaaataac aggcgagaat atgattggtg caaatggcag
    46201 gagtgtacct gaaatggcac ttcccgaaag ctataactat atccacaagt cgggaacttt
    46261 gcatgaagcg ccaagcccta tcattccgct aaattggtcg aaagcttcaa tgactctcat
    46321 gttgaaagag atgtccaacc ttatcaatga tgagggaatt aaatagtttt cataatgagt
    46381 tacagcacaa cgttgggtat tgactgagca agatgaacga caaggacaac gacatcagcc
    46441 ttatgaggag caagaaaatg aaagcaaatt cgaaaacgat cagaaggatg aaagttaatg
    46501 ttcggtctga ggaaagtaaa tcgaaacact catacgtttg aaaccacttt tcaaaattgg
    46561 aaagaaaatg gcgaagatgt tgcattatta atgcccgaat ttagtgctaa atggttaccg
    46621 atagcagaag agagtggcag ctggtctgga tgggtattgc ttggagaaat atttccctta
    46681 atttcatctg agctcgctgg gatggcttta atgccagaaa cggaaaggtt aataggggaa
    46741 tggctcagtt tatccagttc cccgttaaat ctaaagcatc ctgaactaaa atataatcgt
    46801 ttgcacgtag gtaaagtatt tgatggagta ttgaactctg ctcaaccact aataagaata
    46861 tgaacagggg aattgaatct ttggttagat aaagtcacag tctgccaata cggaaacgct
    46921 ccagtgttag acaaaaaatc attatattgg tccattcatt ttgtaattgg atttagtaaa
    46981 acatgttaca gaagtcttgt tgatattgaa gttggtgatg ttttattaat ctcaaataat
    47041 ttggcttatg cggttattta taatacaaaa atttgtgatt taatttatcc agaggagtta
    47101 aaaatggctg atcattttga gtacgaggaa gattttgaaa cagatgattt tgatatcaaa
    47161 aaaaacgaga gtgagattt
//
LOCUS       DAZFYC010000021        46856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFYC010000021 DAZFYC010000000
VERSION     DAZFYC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.021"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            366..491
                     /gene="timP"
                     /locus_tag="ACHKW5_001395"
     CDS             366..491
                     /gene="timP"
                     /locus_tag="ACHKW5_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO7510817.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(523..1299)
                     /gene="sseB"
                     /locus_tag="ACHKW5_001396"
     CDS             complement(523..1299)
                     /gene="sseB"
                     /locus_tag="ACHKW5_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO7510818.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1441..2724)
                     /gene="pepB"
                     /locus_tag="ACHKW5_001397"
     CDS             complement(1441..2724)
                     /gene="pepB"
                     /locus_tag="ACHKW5_001397"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO7510819.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLALRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2902..3102)
                     /gene="iscX"
                     /locus_tag="ACHKW5_001398"
     CDS             complement(2902..3102)
                     /gene="iscX"
                     /locus_tag="ACHKW5_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO7510820.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3114..3449)
                     /gene="fdx"
                     /locus_tag="ACHKW5_001399"
     CDS             complement(3114..3449)
                     /gene="fdx"
                     /locus_tag="ACHKW5_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO7510821.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3451..5301)
                     /gene="hscA"
                     /locus_tag="ACHKW5_001400"
     CDS             complement(3451..5301)
                     /gene="hscA"
                     /locus_tag="ACHKW5_001400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO7510822.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5318..5833)
                     /gene="hscB"
                     /locus_tag="ACHKW5_001401"
     CDS             complement(5318..5833)
                     /gene="hscB"
                     /locus_tag="ACHKW5_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO7510823.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5929..6252)
                     /gene="iscA"
                     /locus_tag="ACHKW5_001402"
     CDS             complement(5929..6252)
                     /gene="iscA"
                     /locus_tag="ACHKW5_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO7510824.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6269..6655)
                     /gene="iscU"
                     /locus_tag="ACHKW5_001403"
     CDS             complement(6269..6655)
                     /gene="iscU"
                     /locus_tag="ACHKW5_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO7510825.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6683..7897)
                     /gene="iscS"
                     /locus_tag="ACHKW5_001404"
     CDS             complement(6683..7897)
                     /gene="iscS"
                     /locus_tag="ACHKW5_001404"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO7510826.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLSGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8009..8497)
                     /gene="iscR"
                     /locus_tag="ACHKW5_001405"
     CDS             complement(8009..8497)
                     /gene="iscR"
                     /locus_tag="ACHKW5_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO7510827.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8767..9507)
                     /gene="trmJ"
                     /locus_tag="ACHKW5_001406"
     CDS             complement(8767..9507)
                     /gene="trmJ"
                     /locus_tag="ACHKW5_001406"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO7510828.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9626..10429
                     /gene="suhB"
                     /locus_tag="ACHKW5_001407"
     CDS             9626..10429
                     /gene="suhB"
                     /locus_tag="ACHKW5_001407"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO7510829.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10574..11428
                     /locus_tag="ACHKW5_001408"
     CDS             10574..11428
                     /locus_tag="ACHKW5_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7510830.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11619..12899
                     /gene="csiE"
                     /locus_tag="ACHKW5_001409"
     CDS             11619..12899
                     /gene="csiE"
                     /locus_tag="ACHKW5_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO7510831.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12891..14030)
                     /locus_tag="ACHKW5_001410"
     CDS             complement(12891..14030)
                     /locus_tag="ACHKW5_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO7510832.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14190..15080)
                     /gene="hcaR"
                     /locus_tag="ACHKW5_001411"
     CDS             complement(14190..15080)
                     /gene="hcaR"
                     /locus_tag="ACHKW5_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO7510833.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            15216..16577
                     /gene="hcaE"
                     /locus_tag="ACHKW5_001412"
     CDS             15216..16577
                     /gene="hcaE"
                     /locus_tag="ACHKW5_001412"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO7510834.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16574..17092
                     /gene="hcaF"
                     /locus_tag="ACHKW5_001413"
     CDS             16574..17092
                     /gene="hcaF"
                     /locus_tag="ACHKW5_001413"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO7510835.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17092..17412
                     /locus_tag="ACHKW5_001414"
     CDS             17092..17412
                     /locus_tag="ACHKW5_001414"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO7510836.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPSTDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17409..18221
                     /gene="hcaB"
                     /locus_tag="ACHKW5_001415"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17409..18221
                     /gene="hcaB"
                     /locus_tag="ACHKW5_001415"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO7510837.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAEKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18231..19433
                     /gene="hcaD"
                     /locus_tag="ACHKW5_001416"
     CDS             18231..19433
                     /gene="hcaD"
                     /locus_tag="ACHKW5_001416"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO7510838.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19530..19952
                     /locus_tag="ACHKW5_001417"
                     /pseudo
     CDS             19530..19952
                     /locus_tag="ACHKW5_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
     gene            complement(20000..20872)
                     /locus_tag="ACHKW5_001418"
     CDS             complement(20000..20872)
                     /locus_tag="ACHKW5_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO7510839.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(20884..21978)
                     /locus_tag="ACHKW5_001419"
     CDS             complement(20884..21978)
                     /locus_tag="ACHKW5_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7510840.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(22011..23009)
                     /locus_tag="ACHKW5_001420"
     CDS             complement(22011..23009)
                     /locus_tag="ACHKW5_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7510841.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23034..24545)
                     /locus_tag="ACHKW5_001421"
     CDS             complement(23034..24545)
                     /locus_tag="ACHKW5_001421"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO7510842.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGMLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24568..25551)
                     /locus_tag="ACHKW5_001422"
     CDS             complement(24568..25551)
                     /locus_tag="ACHKW5_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO7510843.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25648..28928)
                     /locus_tag="ACHKW5_001423"
                     /pseudo
     CDS             complement(25648..28928)
                     /locus_tag="ACHKW5_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29046..30239
                     /locus_tag="ACHKW5_001424"
     CDS             29046..30239
                     /locus_tag="ACHKW5_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7510844.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(30303..31556)
                     /gene="glyA"
                     /locus_tag="ACHKW5_001425"
     CDS             complement(30303..31556)
                     /gene="glyA"
                     /locus_tag="ACHKW5_001425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO7510845.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31884..33074
                     /gene="hmpA"
                     /locus_tag="ACHKW5_001426"
     CDS             31884..33074
                     /gene="hmpA"
                     /locus_tag="ACHKW5_001426"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO7510846.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33119..33457)
                     /gene="glnB"
                     /locus_tag="ACHKW5_001427"
     CDS             complement(33119..33457)
                     /gene="glnB"
                     /locus_tag="ACHKW5_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO7510847.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33518..34852)
                     /gene="glrR"
                     /locus_tag="ACHKW5_001428"
     CDS             complement(33518..34852)
                     /gene="glrR"
                     /locus_tag="ACHKW5_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO7510848.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQIKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34842..35555)
                     /gene="qseG"
                     /locus_tag="ACHKW5_001429"
     CDS             complement(34842..35555)
                     /gene="qseG"
                     /locus_tag="ACHKW5_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO7510849.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(35720..37147)
                     /gene="qseE"
                     /locus_tag="ACHKW5_001430"
     CDS             complement(35720..37147)
                     /gene="qseE"
                     /locus_tag="ACHKW5_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO7510850.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37722..41609)
                     /gene="purL"
                     /locus_tag="ACHKW5_001431"
                     /gene_synonym="purI"
     CDS             complement(37722..41609)
                     /gene="purL"
                     /locus_tag="ACHKW5_001431"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO7510851.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            41867..43423
                     /gene="mltF"
                     /locus_tag="ACHKW5_001432"
     CDS             41867..43423
                     /gene="mltF"
                     /locus_tag="ACHKW5_001432"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO7510852.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43420..43923)
                     /gene="tadA"
                     /locus_tag="ACHKW5_001433"
     CDS             complement(43420..43923)
                     /gene="tadA"
                     /locus_tag="ACHKW5_001433"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO7510853.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGAHDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(43981..44616)
                     /gene="yfhb"
                     /locus_tag="ACHKW5_001434"
     CDS             complement(43981..44616)
                     /gene="yfhb"
                     /locus_tag="ACHKW5_001434"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO7510854.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            44825..45673
                     /locus_tag="ACHKW5_001435"
     CDS             44825..45673
                     /locus_tag="ACHKW5_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO7510855.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRQSEA
                     LMKKLV"
     gene            45988..46254
                     /locus_tag="ACHKW5_001436"
     CDS             45988..46254
                     /locus_tag="ACHKW5_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510856.1"
                     /translation="MRRKESNVSSLPELTNFEVSYSLVTNEVYLSASFTDNMACIPNW
                     PLQEFPDQLICISRAKAVALIEELQKTINYMDAGIDRSSGSLLQ"
     gene            46313..>46856
                     /locus_tag="ACHKW5_001437"
     CDS             46313..>46856
                     /locus_tag="ACHKW5_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510857.1"
                     /translation="MSEFSNLLNSIPGWLSSSLTALVGTLIGGWFTLKGVTQQAKLSK
                     VETERESLELQLSVLKGVKGEVFTLINLYNKRMKTHIDNIKPGQMLILTFPVGDDNFT
                     FYEQNANVIAKLNDSARDSIINIYTYSRSLIQSFKGNNKLIEDYEKILIGMADNNKDK
                     TIYKRLHDAKINVMVDYAQGI"
BASE COUNT        11214 a        12792 c        11783 g        11067 t
ORIGIN      
        1 aaaagatatt acttaaacat gtaaattcac tttcctttaa aaaacaaaaa accgccaaaa
       61 tcaggcggtt atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta
      121 gtaatgcaag cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca
      181 aaagagagtt attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg
      241 caagcgggcg ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag
      301 cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc
      361 gcacaatgaa gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc
      421 aagtaaataa taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga
      481 gcagcaagta agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttctg
      541 tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt
      601 aataaagtgg ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc
      661 gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc
      721 atcggcttca atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca
      781 aataaaagca cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat
      841 ttgtgctggc ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc
      901 ctggctgctc agcggatttc cctctccacc aatcaacaaa ctgatttcac gcggcataaa
      961 ttctttaccg gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa
     1021 caatgtgcga acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc
     1081 ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa
     1141 atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac
     1201 ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt
     1261 ttccagcagg tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac
     1321 gttcggggcg caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt
     1381 gttgaatttg cagaatttgt aggccggata aggcatttac gccgcatccg gcatattagt
     1441 ttacgccgtt aacagattag caatcgtgcg cacaccaagt cccgtagcac ccgcagacca
     1501 ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg
     1561 atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc
     1621 cgcgcttccg gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc
     1681 cgccagcggc agacgccaga atggttcgtt ctcttgtgag gcactcgcca gcaaacgacc
     1741 ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc
     1801 cgccccggtg agggtcgccg catcaatgat cagttccggt ttctgcgcac tggcatcaat
     1861 cagaccatcg gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt
     1921 accgttgcga taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc
     1981 cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg caaatgccag
     2041 cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt
     2101 ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta
     2161 cactggcgct tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg
     2221 ttctgaaccg cgtccgacgg tgtgcagccc catataacct tgctcacgca gatcttcgcc
     2281 tttggtgatc cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg
     2341 cagtgccagt tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca
     2401 gtcgatgatc atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca
     2461 ctccacttta cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc agcagcgatc
     2521 cgcatcccag ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg
     2581 cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc
     2641 attattaatg ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag
     2701 ggtaatcttc atcgcttctg tcattttagt tatccttctt tgtaaaaggg ccgccagttg
     2761 gcagccccag gatttttccg ctaaatgatt cgcgttgcag gaaggcggca agtgagtgaa
     2821 gccccaggag catagataac tatgtgactg gggtgaacga gcgcagccaa cgcatctgcg
     2881 gcgtgaagca tgacgcggaa attattcggc ctcgtccagc cagactaaca aaatcgcttc
     2941 gaggattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca
     3001 ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag
     3061 tgcttcgcca atttcgcggc tatcggtcca tttaagtccc atactaacct ctgttaatgc
     3121 tcacgcgcat ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg
     3181 cgcgcctggc agcttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg
     3241 tcttcctgct ctgagctttc cggcagcgag tcaaaacctt cacgaacgat gcagtggcag
     3301 gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagagct
     3361 gcgtcgagaa tggtttcacc gctattagct tccagaacag cgccatcagg gcagagatcc
     3421 tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag
     3481 cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac
     3541 gtttttaatc gcttgttcga tggcgtcaac gtcatcgccc tgcgccactt cactcaggtg
     3601 agcggcagca tcgtcaatga cctgacgttc tgcggcgctt aacagcgcgg catcagcagc
     3661 cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat
     3721 tcgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc
     3781 gctatcggtc agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt
     3841 ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg
     3901 cgcaccacca gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca
     3961 gtcctgcacc agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc
     4021 tttaaaggtg gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac
     4081 tttctccacc aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag
     4141 cagcatttcg ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat
     4201 agcgacgact ttatccgggt cgatggaagt cagaggtgga cgaccgaaaa attcgcctac
     4261 ccgttcacgc accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc
     4321 atcagcttct acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg ttcgttttac
     4381 cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac
     4441 gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag
     4501 cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct cgcgaatgta
     4561 atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag
     4621 cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag
     4681 gtcataaaca gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc
     4741 ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt
     4801 gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc
     4861 gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg cagaaacgcg
     4921 caccgggttc agcagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg
     4981 gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag
     5041 gcgtttaaca gaactaatgg tgttggcggt atcaagcgct gcgttagtac gcgcgtcata
     5101 acccaccgaa tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc
     5161 atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt
     5221 gcccaggtca ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc
     5281 actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt
     5341 cgagttgttc ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg
     5401 ccgccgcgtc ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa
     5461 acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga
     5521 tctcgtccag ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag
     5581 tatgctgctc gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta
     5641 acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga
     5701 gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct
     5761 gaaaacgcag gctcagcgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg
     5821 tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa
     5881 aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa
     5941 gctttcgccg caaccacact catctttaac gttcgggttg gtgaatttaa acccttcatt
     6001 caggccttct tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc
     6061 gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac
     6121 aaattccagt acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc
     6181 aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag
     6241 tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg
     6301 caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca
     6361 gttcaagttc ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc
     6421 ctttcaccca ttcggtgacc agggagctgg aagcgatagc agaaccgcag ccgtaagttt
     6481 taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcatcacgt
     6541 cgccacaggc cggtgccccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg
     6601 aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa gccattataa
     6661 attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc
     6721 cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa
     6781 ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga
     6841 tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga
     6901 ggcttgctga cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca
     6961 gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca
     7021 ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca
     7081 ggccgctcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcctcgc
     7141 ccattccgac gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc
     7201 cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgtacatac agcgcaccga
     7261 tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc
     7321 tcaggtcgat aggcagttta cccacgctct gggttgcatc aacgtgatag ataatgccac
     7381 gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta
     7441 cgtgcatgat ggaaacaagg atggtgtcgt cacgcatcgc tgcttcaagt tctttcaggt
     7501 cgataatgcc gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg cgctccagct
     7561 gacggcaggt atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt
     7621 ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag
     7681 aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga
     7741 tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa
     7801 aggttccgtc catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg
     7861 tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca
     7921 ctcaatgcaa ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc
     7981 actccggcct gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt
     8041 gtgtgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct
     8101 ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca
     8161 aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct
     8221 gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc
     8281 tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac
     8341 catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt
     8401 cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg
     8461 cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc
     8521 ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac
     8581 attcccgagt aaattggtca actatttact tgactgattt actcgggtat ttaaccttca
     8641 gtgccatttt tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc
     8701 cgacctacgg ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa
     8761 ctactgttat tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg
     8821 caggatattc aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt
     8881 catcacctgc cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag
     8941 atgaccataa aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac
     9001 ctgctcgccg ttttcctgag ttgccagcca ggccatgcgc acttcatagg caataacctg
     9061 aaccgccatc gccaggttca gcgagctgta ttccgggtta gccgcaatcg cgacatgata
     9121 atggcatttc tgcaactctt cattggtcaa gccgacgcgc tcacgaccaa acaccagcgc
     9181 caccggggta tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat
     9241 cggccacggc agcgtgcggg agcgtgcgct ggtgcccacc accaggctac aaccggctaa
     9301 cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc
     9361 cagggcaatc gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa
     9421 tcccattgtt ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac
     9481 cagcacaatt cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc
     9541 ttatcataaa acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt
     9601 ctttaacatc cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc
     9661 gcgcaaggcg ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag
     9721 ccagaaaggc agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat
     9781 cgacacgatt cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact
     9841 tgaaggtact gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt
     9901 tatcaaacgt ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga
     9961 agttgctgtg gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg
    10021 cgcacagctg aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat
    10081 tctggcgacc ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt
    10141 cggcaaactg ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct
    10201 ggcttacgtc gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga
    10261 tttcgcggca ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg
    10321 tggtcataac tatatgctga ccggtaacat cgttgctggt aacccacgtg ttgtgaaagc
    10381 catgctggcg aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg
    10441 ggtgatgtca ctcacccgcc ctcgcctttc aggcgctatt ctgaaatact tcctcaccgc
    10501 tttactttct ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc
    10561 gcaaggaaag gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct
    10621 ctttgttgtt gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta
    10681 tcctgacgat aaaatttacg gtcccgaccc ctggtcggcg gaatccgtcg aatttacggc
    10741 taaggacggt actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa
    10801 cgccatcgca accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc
    10861 gctggtcagt tggttacccg agcgtaattt caacgttttt atgtttgatt atcgcgggtt
    10921 tggtaaatca aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat
    10981 caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag
    11041 tattggcggg gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc
    11101 ggtgatcctc gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg
    11161 cagtggctac ttacttgatg agagttacag cggcgaaaat tatatcgcca gcgtcagccc
    11221 gatcccgctt ttactcattc acggtaaagc tgatcacgtt atcccatggc agcacagcga
    11281 aaagttgtat agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca
    11341 cattgatgct ttttccgatc gccacggcga tgtttatcgc gaacagatgg tggactttat
    11401 ccttagcgcg ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa
    11461 aaccacctta agacatgcta atccactggt cagaacagtt taagatgaga aaaattctgt
    11521 gacgcttgcc aacatttctg atgattagca ttcccttcgt catttccctg agcaaacttt
    11581 agctattctt atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac
    11641 catctgtcct ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg
    11701 ggttgaccgc caccacggca actttcagcg agcttaatgg tgtggatgat gatattgcca
    11761 gtcttgatat cagcgaaaca ggacaggaga tcctgcgcta tcatcaactc acactgacgg
    11821 ctggttatga cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat
    11881 ttcactggct acgacgtggt ttccgtctgt gtccatcatt tattaccagc catttcaccc
    11941 ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg taacttttat gacgatacca
    12001 atctacaagc gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaaacgcgcg
    12061 atattcattt cctgtgtctg tatctgcaat attgtttgct gcaacaccac gctggaatta
    12121 cgcctcagtt taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag
    12181 cgcaggaaat tggacgccac tggcagcgtc gggcgctcca gcctgtacca cctgatgagc
    12241 cactgtttat ggcactactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc
    12301 atcagcgaga cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc
    12361 tgggaaatgt tcgtttttat gatgaacagg ggttatgtga tcagctttat acccacctcg
    12421 cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt
    12481 tcgccagact gtacccacgc ctggtgcgca ccacccgcgc ggcgctggcc ggatttgaaa
    12541 gtgaatacgg cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg
    12601 cctggctaat gcaggaaaac gatctgcatg aaaaacagat tattcttctt accgggaatg
    12661 atagcgagcg agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca
    12721 acattaagca tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac
    12781 tgattattgc gccttatacc atgccattac cgctcttttc accaccgctg atctatacgg
    12841 acctgacgct gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag
    12901 ggaacaactt tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat tacccagaac
    12961 acgccgtggc ccagatattg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg
    13021 ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct accctggcga
    13081 gcagcaatat aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt
    13141 tgcaccacta tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg
    13201 ccgcaaatcg ccgagatcaa cagcatatcg cgtgcactac aacggcggaa aagtttatta
    13261 ctcagcgcaa agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc
    13321 gaggccgagt agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt
    13381 gccccctgca ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac
    13441 cacgctgacc aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga
    13501 cggatgagaa agccgagcag catggatgcc acgcccaacg tcaacagcgc gaggatcacc
    13561 cgataatcaa acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc
    13621 gagccccaca gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc
    13681 agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc
    13741 atcagccacg ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tgtcagcagt
    13801 gccagcacgc gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc
    13861 aaactcccga gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc
    13921 gttaaaccaa tccctttaag ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa
    13981 aagtatgtga aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc
    14041 cgtcaggagg cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata
    14101 ttcgagcctg cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc
    14161 gcgacgcatg ttgcaaaaat aatgcgtaat tatgccgtta cgtttgccaa acgttcctgc
    14221 acaatggcga tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt
    14281 aaagcaatgg aaggcacatt tccggcaatg ggacgaaaaa caacctgtcc ggtattaaaa
    14341 ttattcatat aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc
    14401 accagaatat tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac
    14461 gctttaacga tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg
    14521 ccatccagtt gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac
    14581 acaaccacta atggttcgtc aaaaagctcc agataatcaa tctccgggct ataaacagga
    14641 tggcgcatta agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg
    14701 attaaactca ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact
    14761 tttggcagta aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc
    14821 tgaacaattt tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg
    14881 tcctggagaa aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt
    14941 aacggaacac cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag
    15001 gtatgcagtt tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag
    15061 cgtaaatgcc gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag
    15121 aaattaacta tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga
    15181 aggtcaatac atctgatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca
    15241 tttaccaact gattgatacc caaaatggtc gggtcactcc gcgtatttat accgacccag
    15301 atatttacca actggagctt gaacgtattt tcggccgttg ctggctattt ctcgcccacg
    15361 aaagccagat cccaaaacct ggtgatttct ttaacaccta catgggggaa gatgcggttg
    15421 tcgtagtgcg tcagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg
    15481 ccatgcgtgt gagttatgca gattgcggca acactcgcgc ctttacctgc ccgtatcacg
    15541 gctggtctta tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc
    15601 cacaagggtt gtgtaaatcc cactggggac taaacgaagt tccttgtgtg gagagttata
    15661 aagggctgat ttttggcaac tgggatacca gcgcaccggg cctgcgtgat tacctgggtg
    15721 acattgcctg gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg
    15781 gcggcgtaca aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca
    15841 gtgaccagta tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag
    15901 atgatggcag cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg
    15961 ccaaagatgc gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa
    16021 aaccggatgc taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg
    16081 ccgaagcaga acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata
    16141 ttttccccac gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg
    16201 gccctgatca agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag
    16261 ttaaagccgc ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc
    16321 aggatgactc ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca
    16381 acagcaaact gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc
    16441 ctggcattac taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg
    16501 ccgatcttct gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc
    16561 aggaggtgat gaaatgagtg cgcaagtttc actagagtta catcaccgca ttagccagtt
    16621 tctctttcac gaagccagct tactggacga ctggaaattt cgtgactggc tggcgcagct
    16681 cgacgaagag attcgttaca ccatgcgcac cacagttaac gcgcaaacac gcgaccgccg
    16741 caaaggcgtc cagccaccga caacctggat ttttaatgac accaaagacc agctggagcg
    16801 gcgaatcgcc cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcacccg
    16861 tcacttaatc agcaactgcc agataagcga aaccgacatc ccaaacgtat ttgctgtgcg
    16921 ggtaaattat ctgctttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac
    16981 gcgtttcgac aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat
    17041 cgtcctggat caagcggtaa tcacttccca taacctgagt gtactgttct gatgaatcga
    17101 atttatgcgt gtcccgttgc ggatgtgccg gagggtgagg ctctccggat cgatacctcg
    17161 cccgtcatcg ccctgttcaa cgttggcggc gagttttatg ccattaacga tcgttgcagc
    17221 catggtaatg cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg
    17281 ctacacgccg ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc ctccaccgat
    17341 ccgctcacca cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag
    17401 gcgcagccat gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat
    17461 tagggctggc gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac
    17521 tgtcggcgga aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg
    17581 aaggtaacgt gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgactcgtt
    17641 ccggcaagct ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg
    17701 ttaatactcc cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac
    17761 tcggttacct gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca
    17821 tgattttcac actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg
    17881 ccagtaaaca tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag
    17941 tgcgggtgaa tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc
    18001 tcgggcaaag tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt
    18061 taccgctgca atttttcccg caaccggcgg attttacggg tccgtatgtg atgttggcat
    18121 cgcggcgcaa taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga
    18181 ttcgcggcat tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa
    18241 aaaccatcat tattgtcggt ggcgggcaag cggcggcaat ggctgcggcc tcgctacgcc
    18301 agcaagggtt caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac
    18361 gacctccact ctcgaaatcc atgttgctgg aagattcccc gcagttacag caggtgttac
    18421 ccgctaactg gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc
    18481 tgggtcgcga cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc
    18541 tttttatagc aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac
    18601 gctgctttac cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg
    18661 aacggtcagt cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca
    18721 cgcagcgcag atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg
    18781 caccaccgcc cgtgcaacgc tatcttttac agcgccacca gcaggctggt gtgcgcattc
    18841 tgctcaataa tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa
    18901 gtggggaaac gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc
    18961 aactggctcg cgaggccaac cttgatactg ccaatggcat tgtcattgat gaggcttgcc
    19021 gcacctgcga tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg
    19081 gtgcactaca ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg
    19141 ccgcaatgtt agggctaccg ctaccgctac tgccgccgcc gtggttctgg agcgatcagt
    19201 acagtgataa cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggca
    19261 acccggaaac tcagaaggcg atttggttta atctgcaaaa cggcgtgctt atcggtgcag
    19321 tcacgctgaa tcaggggcgt gagattcgcc caattcgcaa atggatccag agcggcaaaa
    19381 cgtttgatgc gaaactgctg atagatgaga acatcgcgct taaatcactg taaccaggat
    19441 aattagcgaa tatctcaatg cctggggcgt ggcgaggtgc aagagtgtgt attacgttta
    19501 aatcacataa tcttgcaaag ggattggtta tgaacacact acgttatttt gattttggag
    19561 ctgcccgccc cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt ttcattattt
    19621 ttggttttcc caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgttgggcg
    19681 cgccgatgcc gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat
    19741 taatcgtgct tggctttttc acccgtccgc tggcggtgct gtttattttc tacacgctgg
    19801 gtacggcggt gattggtcac cattactagg atatgaccgg cgatgcggtt gggccaaata
    19861 tgattaattt ctggaagaat gtcagtatcg ctggcgcatt cctgttgttg gcaattaccg
    19921 gaccgggggc gatttctctc gatcggcgtt aggaaagatg ccggatgcgg catgaacgcc
    19981 ttatccggca ttttataaat tacagcaacg caacccgcaa cgacatctct gaagttgttg
    20041 attcacctgg tgcaagcgca atgagatcac ccccttccgg gcgatgatga tcatccggcg
    20101 catggctcat cggttccaga cagaagaaat caaacgcata tcctttatca aacgcagggt
    20161 cggaaacaaa gatgaaataa cacggtgcag gtggcgtcgt ttccatgatg atggcataac
    20221 cttcctgcgg ctgctcgatg cgggcctgac cattccatcc ggcaaaacca ttgttcaccc
    20281 actggcgcgg caacggcgcg ggctggttaa aatccagttc ctgcggtagc tgctcgcaaa
    20341 actcacccgc cagccactgc tcccgctcca gccagtaacc gctcgcctgt gcctgaatcc
    20401 gcgtttgcgg cgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg
    20461 ccccttgatt ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct
    20521 gacttacccg atagtgatag acaccgctgc gatgttcata caccagacac aaactatcat
    20581 cgctgtgcga gacacattgc cactcgccca gccagccatc gccgtgtaga tagtgcgcat
    20641 cccactcaac attcggttgc agttgatact cacgcccctg ccagacaaaa cgattgccgc
    20701 tcacccggtt tgcaaatggc accagcggaa aacatgaggc atcggttgcc acaccgcttt
    20761 ttttaccagg acgtaagagc ggcgtcgtat cgcgccagaa gccttcgatt acgccgccct
    20821 ggtcagaaac gtccagcttt agcgacccgt gggataaggt atagatggtc atccgctaac
    20881 tccttaatcc gggaagttaa tcacaacttt cccgcatttg ccgctcgcca tcagcgcata
    20941 ggcatcacct gcctgttcca gcgagaagcg atgggtaatg gcgttacgcg gccacagctt
    21001 ccagtccgtc agatcatggg cgcatttttc catatggaac agactggtca cccaggagcc
    21061 gataatccgc cgttgatggt gcatcagatc ggcgctgacc tcgaattcca cttttccggt
    21121 ttcaccaatg taaaccaccc gtccccagtc agcggtggat tgcagtgcca gcaagcgacc
    21181 tgcggcatta ccggaacaat cgagcgcaac atccgcgcca ccgtgggtga gttcggcgat
    21241 aatctgcggc agaccttcgg tggttgctaa atagccgtga tccatcaccc ctaactgttt
    21301 tgccatcgcc agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc
    21361 tttcgccagc atcatcgcca tcatgccgac cggccccaga ccgaccacca gcacgttatc
    21421 gctgccggaa acttcgccgc gcaaaatccc ttcataggcc gtgccaacgc cgcaactgat
    21481 aaacgcacca tcttcgtagc tcagcgcatc cggcaggagg atcagatctt tttcttccgc
    21541 cagcagatat tcggcatgac cgccgtcacg ctgccagccg taagccgctt ttcctttgcc
    21601 agtacaagag ataggaaagc cgcggcggca gttcgggcaa aaaccacagc cagaaatgtg
    21661 atacaccagc acgcgatcac cctctttaaa atggcgacag ccttgcccca tcgccacaat
    21721 ctgcccgcac ggttcatgac cgttgataaa tccctggtat aacggtttat cgggtgccgc
    21781 cgccgtggca cggtgttgat gatagatata gtggacatcg cttccgcaaa tcccggagga
    21841 tttcattttg atcagtacct ggttaatccc cggcgtcggc accgcaactt cccgcagatc
    21901 gacggttgaa tttcctggta aataagctgc cagcatcgtt ttcataaatc cctcattaac
    21961 aatacgatta attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt ttagcgtttc
    22021 gctttactgc ttcgctgggt cagcaagata ttcgccagca ccgccaccac gatgatgacg
    22081 ccgcgtacca cctgctggaa aaaggagtta ataccgagca gcaccagacc gttaccgatt
    22141 agcgtaatca ccagcacacc aagcaatgta ccgaacaggg aaccgcgacc gccggaaagt
    22201 gccgtaccgc cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt tgcggcacct
    22261 gcgttaccag aaccgaggcg cgccgccaac aaaatgccgg tcaccgccgc taataatccc
    22321 gaaagggtaa agataagaat gcgcacccga cgaacgttga tgccgcacaa ctgcgccgcc
    22381 gtagcattac cgccaacggc aaaaaccgag cgcccgaagg cggttttgcg gctgatgaac
    22441 acaaacagcg caaacaacac aatcatgatc agcgcggata ccggcacacc gagaaattgt
    22501 ccgcccagcc agtccagcac ctcgttttcg tcaatcggca ctggcagtgc gttcgtcata
    22561 aacagcccca ttccgcgcag ggcgctccac agccccagtg tggcaacgaa acttggtacg
    22621 ttaaacacgc cgcgcagcac cccggccagc gttcccatca gcgcgcctaa cagcaacacc
    22681 agcagacacg ccaccgccag cggaacttca aattgcagca aaaatgccag gcacaccgag
    22741 acaaaagcca ccatcggccc aacgctgaca tcaatttcac cggagataat aatcagcgtc
    22801 atcgcccagg cggcaatccc aatggtggcg gcatcgcgca gcacgttcat ctggttattc
    22861 aatgagataa agccaggcgc attcagggag aagaccagat aaagaatggc gattaccacc
    22921 agcaaaccga tctcattaat atggcgactg acaaattgtt tgagcgagac gctcttgccc
    22981 tgcggcaatg ataatgacga agcagacatc ttcaggttcc tcggtaaaat tcatcagtgc
    23041 acagacagaa tggcggacat cagctcatcc acattgaccg gcgagtgaaa ctcctgcgag
    23101 aacgtaccgt gctgtaacaa caggatgcgg tcgcacacca gcggcaactc ctccacttca
    23161 ctggagataa acaccacgct ttttccttcg gcagccagct cacggacaat acggtaaatc
    23221 tgctgtttgg cttcgatatc gacgccgcgc gttggctcgt cgagcaacaa aatctggctg
    23281 gcggcataga cccaacgacc gatcaccact ttttgctgat tgccaccaga aagcatgccg
    23341 atgggcgttt cgctactggc ggccttgacc gtcatccgct gcatcacctc ttcggtcagg
    23401 cggcggatgg tggaccattg cagcactccg ttggcgctga ttttttgccg attggtcagc
    23461 actgtatttt cgtcaacgcc caaccaggga atgatccccg cttctttgcg gttttctggc
    23521 gtatagccaa tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcgtgat tttctcgccg
    23581 ttgataacaa tttcgccctg ttcatacgtc tccagcccaa caatcgcctt cagcaattca
    23641 ctgcgccctg cccccagcag gccagcaatg ccaagcactt cgccgcgacg taacgtaaag
    23701 ctgatatcct ccagcttggg cttatggcgt aacgcacgga cttccagtac ggcctgatcc
    23761 acaatttcct gaggggcaac cggcgcaata tcaacgtgat cacgccctag cattagcgac
    23821 acaatatgat gcgtggaggt gttttcgagc atcacatcgc ccgccacctg accatcgcgc
    23881 ataacggtgg cacaggaggc aatgcggcgg atttcttcca tccggtggct gacataaatc
    23941 accgccacgc ccagtgccga catctttttc accgcactga tcaccagttc aacttctgca
    24001 ctggcaagcg agctggtggg ttcatcaaga atgaccacgc gcggctcgcc cttcatcacc
    24061 cgtgcaattt ccaccagttg cttttgcgcc gggcttagcg ttgaaacaag ttgttcagga
    24121 ctaacgtcaa cgcccagcgc ctgtaagcaa cgttgggcat cctgcgccat ttgcaggtaa
    24181 tcaatcatgc cgttgcggcg aggccattga ccaaggcaga ggttttctgc caccgtcagc
    24241 ccttccacca gacttaattc ctgataaacc gcacgaaccc ccagttcagc ggcacggcga
    24301 gtcaacgtag cttcgtcacc ttccagtcgc gtctcgccaa tccagatatc accgctatcc
    24361 gggcgttcgc tgccggtaag cattcgaatg agagtcgatt tgcccgcgcc gtttttacct
    24421 aacagcgcac gaacttcgcc tttattgagc gtgaagttaa cgttatccag cgcaacgacg
    24481 ccggggtaac gcttatttcc tgccaccact tttgctaccg ggactgcctc tgttgccgta
    24541 aacataggga cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg ttgccagcca
    24601 ctgtttacca tcttccgttt tggtatagag atcgattggc acctgaatca ctttttcacc
    24661 gtcggcttgt ttattgataa ccttcaatgt ttgcgcgaaa acagcattgc ccattttctt
    24721 accggaaata tccactaccg ctttcagcac ctgattgttt tccagctcct gagcaatttc
    24781 ggttgtcata tccgaaccga aaacagcaat ttttccggcc tgattttgat tacgtaccgc
    24841 ttttaccgcg ccgagtgtcg caccgcccga ctcccccata atggcgttga gatccggcgt
    24901 ggagataatc agtttttcac caacggaaat cgctttatct aaaacagtcc cttcctgatt
    24961 agcgacaatt tgcgcgccgg gaacgcggga ttttaatact tcttcaaatc ctttacgtcg
    25021 ctgcacacaa acttcaaagg cttcgcaatt gatgacggca attttcggct ggtcaatttt
    25081 attggcaata aaataatcgg cggcagcgtt acccagtttt ttaccaaatt ccagtggatc
    25141 gccgaccaga tacgccgaga catatttatc gacacccttt tgattaatac aggtgttgta
    25201 gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac tgccattttc
    25261 agacactgcc gataaaataa tggcatcgac attacgcgcc acgagggtat caacaaaggt
    25321 actttctttc gaaatatcac cctgggcgtt agtttcaatt aactgtacct gtactgaaga
    25381 atcttttgcc gcatcctgaa cgccctgacg cactccagcg taatatccct gggtatcaag
    25441 gtatattgca ccaatggtca tttctttatc cgcagccctg gcaaatagtg cgctacctaa
    25501 tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca tagtgcctcc
    25561 tgtagggttt ttattaacaa cggcttattc taattatttt gtgatgagcg gcagcgcgtg
    25621 ccgctcaaat attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac
    25681 ggtggtgaat aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc
    25741 agattcatat tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca
    25801 tttttcttta tgctgctgtt gtaaatcgcc atacagcgac aacaggtcag gttgtgaaac
    25861 agcaaagaaa tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc
    25921 actaaacagt tgctgtgcga tttgttgttc gcccagcagt cgcagcgcca ttccttgcca
    25981 gaagagataa tcaaccggct gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc
    26041 ggtcgccgcc agacgtaaac aacgcgtcgc ttcagtttca tcgccctgcg cgttggcgca
    26101 tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa
    26161 attctccgga taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctgtctggc
    26221 atcaaggtgc tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc
    26281 ttccccgcct tcccacgggt ggaatttgcg cgtggcgaga atgtccgccg ctttgtctgc
    26341 ctgacccgtg agatgccaca aattgagcaa ctctgctgtc atgtcgtcgc gtttcagcgc
    26401 aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cgcttaactt
    26461 atcaagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt
    26521 atcaagataa cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa
    26581 cccgcgccag ccgtcggcaa actccggcga catctctacg caacgttgcc ataaggtaat
    26641 ggctttgttg tagctacgct tgttgtagta gaagcaggcc agtaaatgac gggcaaacca
    26701 gcactcttca atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa
    26761 ctgcgggaag acatcaatgg cttttgcgac cagttcgccg cgttcggctt tcggcaataa
    26821 actcgcctgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc
    26881 cgccagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcagag cgttaacgtc
    26941 gcgtccctga cacagaccgc gccactgcgc gagggctgat tcgctacgac catcgaacca
    27001 gttcagccac cacagagtgc gttcagcgga taatcacgca gcagtttcgc gcgctgcaaa
    27061 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaggca aagcacttcc
    27121 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagaccagc gtcgaagtta
    27181 ccattacgcg ccgccagtcg tgccaggcca taatagccac cggctttgct gttgccgctc
    27241 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27301 ctggcgcgaa tcaaactggc ctgtccgcac tgcggatttt tgttcagcgc atgtgcgcgt
    27361 ttcagagcct gagtggcata cgccaccgct tgcgggaaat cggcgcggtt atattccagc
    27421 atcgccagcg ccaggttaca gcgataatcc agcggatcca gcaccacgcc gcgcaggtag
    27481 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    27541 gcttcatctg tactggtaat gtcttgtgct gcgagtggtg ctttggcgac gtccggcaac
    27601 ggcaactctt gcggctgatg ttcctgataa ctcaggacaa tattgccgtc ggcatcggag
    27661 agctcaatgg tcagcctttc aggattgata ccgtgcaaca cgccctggat ggcggtcgca
    27721 ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt tgccgatttc gcggatcgcc
    27781 aggcggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    27841 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccaa agaatgataa
    27901 ggcaggaaat actgctcaaa acgcttctct tcgtaagcat caagccaggt aaaatcgggc
    27961 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28021 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28081 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgcca
    28141 acaaaatcgt actgtgattt ttcagccata tatgaggttg gaacgggcac atttttatag
    28201 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28261 aaagcggaga cggcccgttt gccgtgatca aacaccgccg ttacatccgg cgggaaaacg
    28321 ctctgatgcc cttccccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    28381 gtggcgttac cgttacagac gcggctggcg atttccagcg ccgcccggtc agggcgcagg
    28441 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgcctac ccacaccgtc
    28501 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    28561 tgatgttgcg gccagttaaa ctcaatccca ccggaaatcc acggccccag cagccccacc
    28621 agcgcaggtt taatgacttc attgtgataa acaaaatcgc gctgtttcac tttatcccac
    28681 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    28741 agccacaccg cctgccagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    28801 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    28861 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    28921 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    28981 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29041 tgaagatgag agcctgcatt aacaatcaac agattcgcca ccataacaaa tgcgtgattc
    29101 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29161 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29221 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29281 aaggtgactg gacgctgtgc ctgaacgtaa cgcccaccag tattgagtgt caggttgcta
    29341 atgcttgttt aagtccgaaa ggtgaatttg agtatttaca gattgatgca ccgacgccgc
    29401 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgtca tcgcaaactg tggccgggcc
    29461 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    29521 aaaccatgcc gcaagcgccg tggacaacgc cggttgaggt gaagtatctg ctggaagaga
    29581 agctgggcat tcgggtgatg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    29641 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    29701 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    29761 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggctgcctgg
    29821 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    29881 cggttaatac tcagcttgat cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    29941 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30001 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30061 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30121 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg ctttgggcaa ttaagccgcg
    30181 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30241 ggcgcgataa cgtagaaagg cttcccgaag gaagcctttt ttcaacagat taaccgtttc
    30301 gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt gatgcgctcg
    30361 ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc cagttctttc
    30421 gcttctgctt ctttgaagcc gcgacgcgta attgccggag tacccacgcg aataccggag
    30481 gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt gatgttagca
    30541 cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcgaccag atcaaccagg
    30601 aacagatggt tatcagtacc gccggaaacc actttgtagc cgcgttccag gaacacttct
    30661 accatcgctt tagcgttttt agcgacctgc tgctggtaag ttttgaactc aggctccatc
    30721 gcttctttca gagcaaccgc tttaccggcg attacgtgca tcaacggacc gccctgacca
    30781 ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc tttcgccagg
    30841 atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac aacgtgagca
    30901 tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg cgccatatca
    30961 acgaacaggt aagcaccgat gctgtcagcg atttcacgca ttttcgccca gtccaccacg
    31021 ccggaatatg cagagaaacc accgataatc attttcggct tgtgttcttt ggcttgtttt
    31081 tccagatcgg cgtagtcgat atgaccggta gcatcgatac cgtaaggaac gatgttgtac
    31141 agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg cgccaggttc
    31201 atacccagaa cggtatcacc tggttccagc agcgcggtgt agaccgcaaa gttagcctgg
    31261 gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt cgcacgatcg
    31321 atcgccagtt gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg tttgcccgga
    31381 taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac gcgcgggctg
    31441 gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt ttcctgctcc
    31501 atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt taacatccgc
    31561 atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct gttggacaac
    31621 ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga atttacgcaa
    31681 acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac cggaaacgga
    31741 tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct gaaaaacacc
    31801 aaagaaccat ttacattgca gggctatttt ttataagatg catttgagat acatcaatta
    31861 agatgcaaaa aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca
    31921 ccatcccttt actggtggaa acggggccaa agttaaccgc ccatttctac gaccgtatgt
    31981 ttactcataa cccagaactc aaagaaattt ttaacatgag taaccagcgt aatggcgatc
    32041 aacgtgaagc cctgtttaac gctattgccg cctacgccag taatattgaa aacctgcctg
    32101 cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac cagcttccag atcaaaccgg
    32161 aacagtacaa catcgtcggt gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg
    32221 ggcaggaagt gctggacgcg tggggtaaag cctatggtgt actggctaat gtatttatca
    32281 atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc
    32341 gcgatttccg cattgtggct aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg
    32401 agccggtcga cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc
    32461 tgaagccgga aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg
    32521 atggcaaagg ctatcgtatt gcggtgaaac gcgaagaggg tgggcaggta tccaactggt
    32581 tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt cgctccggca ggtgatttct
    32641 ttatggctgt cgcagatgac acaccagtga cgttaatctc tgccggtgtt ggtcaaacgc
    32701 caatgctggc aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggt
    32761 tccatgcggc agaaaatggc gatgttcacg cctttgccga tgaagttaag gaactggggc
    32821 agtcactgcc gcgctttacc gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg
    32881 ctaaaggtca gtttgatagc gaaggtctga tggatttgag caaacaggaa ggtgcgttca
    32941 gcgatccgac aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttgccgcga
    33001 aacagttagt ggatctgggc gtgaagcagg aaaacattca ttacgaatgc tttggcccgc
    33061 ataaggtgct gtgatttgat gttgccggat ggaaacatcc ggcaaccctt gacgcggttt
    33121 aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat gacccgtgcc acgtcaaaga
    33181 cgaagatttt accgtcaccg attttgccgg tttgcgccgt gcgaataatg gtatcgacac
    33241 aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca
    33301 ccatatactc cgcgccgcgg tacagctcgg tatggccttt ctggcgacca aagcctttca
    33361 cttcggtcac cgtcatgccg gtaataccga cttcggccag tgcttcgcgg acatcgtcca
    33421 gcttgaaggg ttttataatc gcatcaatct ttttcatgct attccttgaa aaggtcgcct
    33481 gtcttttgat ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat
    33541 ccagctcgtg tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg
    33601 ccgcgtgggt gacgttgcct ttggtaattt gcagcagctt acgcaaatag ttgagttcaa
    33661 actgattacg tgcctcaaca aaggttggca gcgccgtgtt ttcaccctcc agcgcctgct
    33721 ctactaatgc atcgctaata accggagatg aggtcagcgc cacgcactgt tcaatcacgt
    33781 tgaccaactg gcgcacatta cccggccagc tagcggtcat caggcgtttc atcgcatcag
    33841 tggagaacgc gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg
    33901 ccagaagcgg aatgtcttct gtgcgctctg ccagagccgg aattttcagg ctgacaacgt
    33961 tgaggcggta atagagatct tcgcggaatt ccccgcgcgc catcgctttt ggcagatcac
    34021 ggtgagtggc agaaataatc cgcacgtcga tatcaatatc gcggttgctg cccagcgggc
    34081 gcactttacg ctcctgcaac acgcgcagca gtttgatctg taacggcgca ggcatatcgc
    34141 caatttcatc gagaaatagc gttccgcctt ccgccgcctg gaataaacct tcgcgattgc
    34201 tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag ctccgactcc agcaattgct
    34261 cgggcaatgc gccacagtta atagcaataa atggtttgct gttgcgcggg ctggcgttgt
    34321 ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg accgttaatc aaaacgctga
    34381 cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg
    34441 tgacaattgc ctcgcgccag cgttcatcgg tggctggcgc ggattgctcc agcgcatcgt
    34501 caattgcctg atatagcgcg tctttgtcga caggcttggt gaggaaacta aaaacgccct
    34561 gctgtgttgc agcaacggca tcggggatag aaccatgcgc agtaagaata attactggca
    34621 ttcccggctg cactttctgg atttcagcaa atagctgcat accgtccatt tcatccatcc
    34681 gcaggtcgct gataactaaa tctacttttt cgcgattcag tacccgtaat ccttcagcgc
    34741 cactttccgc cgtgaccaca ctgtagcctt cgctggtcag gcgcaggcca agcagtttca
    34801 gcaatcccgg atcgtcatcg accaataata aatgcgcagg tttatggctc atcaggagtg
    34861 acctcatggg tggatggcgc gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt
    34921 ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg
    34981 gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg
    35041 ctgtcgctgg actgctggag tttactgtaa cgttggcgct cttccgccag ttgcagttgc
    35101 agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa cctgtgccgg gatctcggta
    35161 cttaacgctt caatacgcgc gaccagctgg cggcgctcat acggcgtaat tttggcgtcg
    35221 gcgagcaaaa tcccttgttt aaaggtattt tgccagctgc cgtcgtcata ttggcgagct
    35281 tgctgacgcg actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa
    35341 agcggattgg tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa
    35401 agatagtcag ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc
    35461 ttattgtgat tctgcacgca acccagcaat gccagacatg gcagccctgc cagccacagc
    35521 cgtcggggca ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc
    35581 agtgggcgta attgttgcag acagtctgga catggacagc gcggagaaac cggagcgtac
    35641 atatcgtacg tgaagatttc gagcactgcc cggggccgaa ctgacaaata aaatagcctg
    35701 ataggatagg ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac
    35761 aaacgtcttg cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat
    35821 ccctggcaat gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc
    35881 cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg cggaattggc gtgccggtat
    35941 tgatgacatc aatataaacc cgcgcaccat gtgaactgct gcgaaggcaa atgttaccgg
    36001 attcagcccc gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca
    36061 ttggctccgc caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc
    36121 gtgcgggcag gctatgagca gaaaccactg tctccaccag cggtgctaat tcaacattct
    36181 ccagttccac cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt
    36241 tttgcaaatt gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa
    36301 gcggcccgac aacctggtca gccagtaatt cagtgccctc gcgcatactc gccagtggcg
    36361 tttttaattc atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca
    36421 ggcgctcact taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga
    36481 acgaaacgct attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat
    36541 ttttcaccgg cccgataatc atccgcgtga aaagtagcac catcaccaga ctcaccagaa
    36601 atagcaccag cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt
    36661 gctgcccacg agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg
    36721 caaaggcttc aagacgcgcg gcggcggcag catcgggacc actgttgtta cactgaagtt
    36781 gggccagatt gttcaaatcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc
    36841 ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa
    36901 gcgtaggatc gtccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt
    36961 tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct
    37021 gatcgctaag cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggtagta
    37081 gaattagcaa aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acaggccagc
    37141 gtttcaaggt gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca
    37201 gatacaacaa agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga
    37261 cgtttcgccc gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt
    37321 gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa
    37381 tgagccactg cttactatat gcagtaactg tgccaataaa gaaaatagtt tggtaacgta
    37441 ctgattatac gtcgctttga ggggtttatg gcttctccgt tatttgaatg taaatcagcc
    37501 actgtgtcgc taaaaagaga caacttaaga taaacttatt agataatatt aaaatcaatg
    37561 agttaagtgt cgccagaaag cgacacggta aaccacccat tgtcgtgatt tacagacaca
    37621 aaaaagctcc cggagttggg agcttatgat agtggttggt gcttaatgcc ggatgcggcg
    37681 taaatgccgc atccgggctg caaaaccaat gaactgatga cttaccccaa ctgcttacgc
    37741 gcattgcgga aaatgcgcat ccatgggcca tcctcgcccc agttttccgg atgccaggag
    37801 ttgctgacag tacggaaaac acgttccggg tgcggcatca taatggtgac tcgaccactt
    37861 tcagtcgtaa tcgacgtaat accgttcggt gaaccgttcg ggttagccgg gtaggtttca
    37921 gtgactttgc cgaagttatc gacatagcgc agtgccacca gccctttgct ttccagtgcc
    37981 gccagatgcg ccgcatcacg cacttccacg cgcccttcac catgagagac agcaatcggc
    38041 atttgcgagc ccaccatccc ctgcaacagc agcgacgggc tttgggtcac ttcaaccagg
    38101 ctgaaacgcg cttcaaagcg atcggaggta ttgcgcacaa aacgtggcca caactcacta
    38161 cctgggatca gttcgcgcag gttagacatc atctggcaac cgttacatac ccccagcgcc
    38221 agcgtttgcg gacggtggaa gaaggttgca aactcatcgc gtacacggtc attgaacagg
    38281 attgacttcg cccaaccttc accggcaccc agcacatcac cgtaggagaa accaccgcac
    38341 gcgaccaggg cgtggaaatc ttccaggccc gtgcgtccag tcagcaggtc actcatatgc
    38401 acatcgattg catcgaagcc tgcacggtgg aaagctgccg ccatttcaac atgcgagttc
    38461 acgccctgct cacgcagcac agcaactttc ggacgtgcgc cagtggcaat atacggtgct
    38521 gccacatctt cgttgatatc gaacgacagt ttcacattca ggcccggatc ggcgtcgttt
    38581 gatttcgcct ggtgctcctg gtcagcacac tccgggttgt cacgcaggcg ctgcatctgc
    38641 caggtagttt ctgcccacca gacacgcaac gtggtgcggc tttcgctgaa tacagtctgc
    38701 ccgttggcgg taatcacaaa acggtcaccg gaaaccgcct gccctacata atggacacaa
    38761 tcagcaagcc catgctgtgc cagtacggac tcgaccgctt cacggtcagc ggcacgaacc
    38821 tgaatcaccg cacccagttc ttcgttaaac aacgccgcca ggcgatcgtc gcccagagag
    38881 gcaatatccg cgttaatgcc gcaatgacca gcaaaggcca tttccgccag cgttaccagc
    38941 aggccgccat cagagcggtc gtggtatgcg agcagcttac gctgtgcaac cagcgcctga
    39001 atcgcgtcat agaagccttt cagttgcgcg acatcgcgta catctgccgg tttgtcgcca
    39061 agctgacgat aaacctgcgc cagcgccgtt gcgcccagcg cgttattgcc tttgcccaaa
    39121 tcaatcagca gcagtgcgtt atcttcggta gaaagctgcg gcgtgatggt gtgacgtaca
    39181 tcttccacgc gggcaaatgc agaaatcacc agcgacagcg gcgacgtcat ttcgcgctct
    39241 tcgttacctt cctgccagcg ggttttcatc gacatagagt ctttacccac cgggatcgtc
    39301 aggcccagcg ccggacaaag ctcttcgccc acggctttaa cggcttcata caggcccgca
    39361 tcttcaccag ggtggcctgc cgccgccatc cagttggcgg aaagtttaat gcgtttgata
    39421 tcgccaatct gtgttgcggc aatattggtt aacgcttcac cgaccgccag acgggcagag
    39481 gcggcgaaat ccagcagcgc aaccggcgca cgctcgccaa tcgccatcgc ttcaccgtag
    39541 tagctgtcga ggctggcggt agtgaccgcg cagttagcga ccggcacctg ccacggaccc
    39601 accatctgat cgcgcgctac catgccagtt acgctgcggt caccgatggt caccaggaaa
    39661 gttttctccg ccacggtcgg cagatgcagt acgcgtttca ccgcgtctgc aatggtgatc
    39721 ccttcacgga ccagcgcatc gcccttcgct ttcagcgttt gtacatcgcg ggtcatcttc
    39781 ggcgttttac caagcaggac gtccagcggc agatcgatcg gctgattatc aaaatgacga
    39841 tcgtgcagag aaagatgcag ttcttcggtc gcttcaccaa tcaccgcgta gggtgcgcgc
    39901 tcacgcttac acagttcgtc aaacagcggt aattgatcgg cagcaaccgc cagcacgtag
    39961 cgttcctggg attcgttaca ccagatttcc agcgggctca tgcccggttc gtcgcttaga
    40021 atatcgcgca gttcaaattt accgccgcgt ccgccgtcgc tcaccagctc cggcatcgcg
    40081 ttagaaagac cgcccgcacc gacgtcgtgg ataaacagaa ttgggttagc atcaccaagc
    40141 tgccagcaac ggtcgatcac ttcctggcaa cgacgctcca tctccgggtt gtcgcgctgt
    40201 acggaagcaa agtcgaggtc ggcatcagac tgacctgacg ccatggaaga cgccgctccg
    40261 ccgccaagac cgatgttcat tgccgggccg ccgagaacga ccagcttcgc accgacgttg
    40321 atctcgcctt tttgtacgtg atcggcgcga atgttgccga tcccgcccgc cagcatgatc
    40381 ggtttgtgat aaccgcgcag ctcttcgccg ttgtggctgt tcactttttc ttcataagta
    40441 cggaagtagc cattcagtgc cggacgacca aattcgttgt taaacgccgc gccgcccagc
    40501 gggccttcgg tcatgatgtc cagcgcggtg acaatgcgct caggcttacc gaaatcttct
    40561 tcccacggct gttcgaagcc aggaattcgc aggttggaaa cggagaaacc aaccaaaccc
    40621 gctttcggct tcgcgccgcg cccggtggca ccttcatcgc ggatttcacc gccggaaccg
    40681 gtcgccgcgc ccggccacgg agaaatcgcc gtcgggtggt tgtgagtttc gactttcatc
    40741 agaatatgcg ccggttcctg atggaaatcg tagcggcccg tttcgtggtc agcaaagtag
    40801 cggcccactt cagaaccttc cattacggcg gcgttatctt tataagcaga gagaacgtaa
    40861 tctggcgtgg tttcgaaggt gtttttgatc atcttaaaca gcgatttcgg ctgttgctca
    40921 ccgtcgataa cccaatcggc gttaaaaatc ttgtggcggc agtgctcgga gttcgcctgg
    40981 gcgaacatat acagctcgat gtcgttcggg ttacgaccaa gctttgtgaa agcatcctgc
    41041 agatagtcaa tttcatcttc cgccagtgcc aggccaagac gcaggttagc gtcgattagc
    41101 gcctgacggc cctgccccag caaatcaacg ctggtgaccg gagtcggttg atggtgtgca
    41161 aacagctgct ctgcatcatc taaagcaaaa aagaccgttt ccatcatgcg gtcgtgcagt
    41221 tcagcggtaa cctgctgcca ttgttcattg gtcagcgtac cggcttctat atagtaagca
    41281 acgccgcgct caaggcggtt tacctgttgt agcccgcagt tatgggcaat atcggtcgct
    41341 ttcgaagacc agggagagat ggtgccagga cgcggggtca ccagcaggag tttgccttgc
    41401 ggggcgtggc tggcgagtgc cgggccatat ttcagcaggc gttcaagttg tgcgtgctca
    41461 tcatcgttta acggcgcatt gaggtcagca aaatggacat actcggcgta aatattgtga
    41521 accgggagcc tggcagcctg aaaacgtgcc agcagtttgt tgattcggaa tgccgacagt
    41581 gcaggcgaac cacgcagaat ttccatcata agtctctcgt cttctaagct ttcggtgtac
    41641 ccaagggggg gaaacgggcg tcattataaa gaatctgatg cgctgacgaa accgtttgcg
    41701 tggaaataaa atcaccatcg tgaattagca acgcgtgccg ccaatggctg taataagttg
    41761 ccatctggcg caggtttacg caaaatgccg ctcatttatg agtaaacctt tcactattat
    41821 tacgtttttt caagctggga cgcgcacgac acagagaatt aactaattga aaaaattaaa
    41881 gattaattat ctgttcatcg gcattctggc actgctgctc gcggtcgctc tctggccatc
    41941 cattccctgg tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc ggggagagtt
    42001 gcgtgtgagc accattcata ctcccctgac ttataacgaa atcaacggga aaccttttgg
    42061 cctggattac gaactggcga aacagtttgc cgattacctc ggcgtaaaac tgaaagtgac
    42121 cgtgcggcag aatatcagcc agctgtttga cgaccttgat aatggtaacg ccgacctgct
    42181 ggcggcagga ctggtctata acagtgagcg ggtaaaaaat tatcagcctg gccctaccta
    42241 ttattccgtg tcacaacaac tggtttataa agtgggtcag tatcgcccac gtacgctggg
    42301 caacctgacg gcggagcaac tcaccgttgc accgggtcat gtggtggtta acgatctcca
    42361 gaccctgaaa gaaacaaaat tcccggaatt aagctggaag gtagacgaca aaaaaggctc
    42421 tgcggaatta atggaagatg tcatcgaagg aaaactcgat tacaccattg ctgattctgt
    42481 cgccatcagc ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg atatcaccga
    42541 tgaacaaccg gtgacttggt ttagcccgtt agatggcgat aatacccttt ccgccgccct
    42601 gctcgacttc ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg aagagaaata
    42661 cctggggcat ggcgatgatt ttgattacgt cgatacgcgc acatttttac gcgccgtcga
    42721 tgcggtactg ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa ttgactggcg
    42781 tttgctggcc gctattgctt atcaggaatc gcactgggat gcacaggcca cttcaccgac
    42841 gggtgtgcgc ggcatgatga tgttaaccaa aaataccgcg caaagcctcg gcattacgga
    42901 tcgtaccgat gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagg atatgatgag
    42961 taaagtgccg gaaagtgtgc cggagaacga gcggatctgg tttgccctcg ctgcgtacaa
    43021 tatgggctat gcgcatatgc tggatgcccg cgccctgacg gcaaaaacca aagggaatcc
    43081 tgacagttgg gctgacgtaa aacagcgtct gcctttactt agccagaaac cctattacag
    43141 caagctgact tacggctacg ctcgtggaca tgaagcctac gcttatgtcg aaaatattcg
    43201 taagtatcag attagcctgg tgggttatct gcaagagaaa gagaagcagg ctacagaagc
    43261 ggcgatgcaa ctggcgcagg attatccggc ggtatcgcct acggagttgg gcaaagagaa
    43321 atttcctttt ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc attctccctc
    43381 tctgctgttt tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg aggattgcgc
    43441 ttttttctgc gctttaattt cctggcggcg catgcgaaag aagtcactga gcaacgctgc
    43501 gcactcatcc gccagtattc cttccgtaat ttccactcgg tgattcatac ccggatgatg
    43561 cagcacatcc attaaagatc ccgcagcgcc agttttcgcg tcatgcgcac caaagaccac
    43621 gcgaccaatg cgactgtgga tcatcgctcc ggcacacatt acacatggtt caagcgtgac
    43681 atacaacgtg gcgtcgatca gacgataatt ttgcatcacc agaccaccct gccgcagggc
    43741 catgatttct gcatgtgcgg tgggatcatg gcgaccaatc gggcggttcc agccttcgcc
    43801 gattacccga ttgttatgca ctaataccgc gccgaccggc acttcccgct catcccaggc
    43861 acgtttcgcc agcgtcagcg cgtgacgcat ccagtattcg tggctaaatt cgacttcaga
    43921 caaaaagaaa actccggtta taaaagcgcg gcgcattata cacggacgct atgctttact
    43981 ctattccagt tgctggagtt caccgcgcgg ggttactcgc caacgatgct gacagaaata
    44041 aagcagcgga ttgtcctgat tactgtcgct atagccactg tacagccgca gcggagtgcc
    44101 gattttgcgc tccagttgtg cgaccttttc atgtcccaga caacgcatcg tcaataccca
    44161 accaccatag ccacgctgaa tttggctggc gataagatta acccgcggca gccagggcgt
    44221 atcgaaataa accgcttcaa ccagcggctg cggagagccg gtaatcaacc agatatcagc
    44281 atcggaactt aacaggtagg tggttaatcg ctcctgaacc agcggaaagg cggtaacatt
    44341 gtcgcgaaac cagcgcacga aatcggcctg caacgtctgt aaacgtgctt cgctgtgacc
    44401 aaaagtgcac ccccacagaa gcagactcat cggccagcgt gccgcacgac cttttatcaa
    44461 taacgcaatg gctataatcg gtaacaacgg caggacaagt aacgcattca gcggttggcg
    44521 acgcagtaaa tagcgcagaa aactgccgaa catatcctgc tgatgcaatg ttccatctaa
    44581 gtcaaaaaac accacacgac gctcgtgagt tgccaaacca tactcctctg gactgaaaca
    44641 tcctgattaa ttttcgctac tacatagcct aacagataga tcatcacttt tccggcaaca
    44701 atcgaccgca tcatggctaa ctggaatttt taattcatgg caattagcgg caatggaata
    44761 taaaattcac tcgcgtgtgt ctcatattgc ctgttgtcgc cactatttca tcgcgaggaa
    44821 agatatgaac ggcttacttc gtatccgtca gcgttaccag gggcttgccc agagtgataa
    44881 aaaactggcg gattatctgc tgctacaacc tgatacggcg cgccatttaa gttctcagca
    44941 actggccaac gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc aaaaactcgg
    45001 ctataaaggt tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa gccagccgga
    45061 atcgccctcc gtgcccattc ataaccaaat ccgcggtgat gatccgttac ggctggtcgg
    45121 cgaaaaactg attaaagaaa atactgccgc tatgtacgca acgctaaacg ttaatagtga
    45181 agagaaactg catgaatgcg taacaatgtt gcgctctgcg cggcggataa ttctgaccgg
    45241 tattggcgct tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga agattggctt
    45301 caatgctgcc gcagtgcgcg atatgcatgc gctactcgca acagtacagg cgtcgtcccc
    45361 tgacgatctg ttattagcca tttcctacac cggtgtacga cgcgagttaa acctggcggc
    45421 agatgagatg ctgcgagtgg gcggaaaagt gctggcgatt accggcttta ctcccaatgc
    45481 cctgcaacag cgtgcttctc attgcctgta taccattgcc gaagaacaag cgacaaacag
    45541 tgcttcaatc tctgcttgtc acgctcaggg aatgttaacg gatttgctgt tcattgcgct
    45601 gattcagcag gatctggaac tggcaccaga acgtattcgt cagagtgaag cgctgatgaa
    45661 aaaactggtc tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt tgttttgaga
    45721 gtttccttat ggcgttgtta atcactaaaa aatgcatcaa ttgtgatatg tgtgaaacag
    45781 aataaaaaca catttatttt catagtgata cagcaatgtg ttattgaatg tgttgttgga
    45841 tttatggtgc aaaaaaattt tgcataagtt cagcaacaca aagatccccc tcccctttct
    45901 ataacggttc atttgagact cccccttgac tccctcttcg aaattgaaga ccatcacctg
    45961 tacataatta cagtcatgga taatattatg cgcagaaaag agtcaaacgt tagcagtttg
    46021 ccggaactca ccaattttga ggtaagctat tctctggtta ccaatgaagt ttacttgtct
    46081 gcatccttca ctgataacat ggcctgtata ccaaactggc ccttacaaga atttccggat
    46141 caactaatat gcatttctcg agcaaaagct gttgccctaa tcgaagagct ccagaagacc
    46201 atcaactata tggatgcagg aatagatcga agttcaggta gccttctcca gtagctgacg
    46261 agttttataa taatttgata tatatatcgt tcaatattag cgagtactta taatgagtga
    46321 atttagtaat cttttaaata gcattcccgg atggttatcc tcctccctca cagctctagt
    46381 tggtactctt attggcggat ggtttaccct aaaaggagtt acccaacaag ctaagctgtc
    46441 taaagtagag acagaaagag aatcccttga attacaactt tcagtattaa aaggagttaa
    46501 gggagaagtt ttcaccctaa tcaatttata taataaaagg atgaaaacac acattgataa
    46561 tatcaaaccg ggacaaatgt tgattctcac attccctgta ggggatgata atttcacatt
    46621 ctatgagcaa aatgcgaatg tcattgcaaa actaaatgat tctgccagag attcaataat
    46681 taatatctac acatattcac ggtcattaat acaatcattt aaaggaaata acaaactcat
    46741 tgaggattat gaaaaaatcc ttattggtat ggctgacaat aataaagaca aaaccatata
    46801 taagagattg catgacgcta aaataaatgt aatggtagat tatgctcaag ggataa
//
LOCUS       DAZFYC010000022        45663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFYC010000022 DAZFYC010000000
VERSION     DAZFYC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.022"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..1208
                     /gene="yiaY"
                     /locus_tag="ACHKW5_001438"
     CDS             57..1208
                     /gene="yiaY"
                     /locus_tag="ACHKW5_001438"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO7510858.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVGRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1373..2911
                     /gene="aldB"
                     /locus_tag="ACHKW5_001439"
     CDS             1373..2911
                     /gene="aldB"
                     /locus_tag="ACHKW5_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO7510859.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3130..3285)
                     /locus_tag="ACHKW5_001440"
     CDS             complement(3130..3285)
                     /locus_tag="ACHKW5_001440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510860.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLIRP
                     LTIETRD"
     gene            3456..3779
                     /gene="yiaW"
                     /locus_tag="ACHKW5_001441"
     CDS             3456..3779
                     /gene="yiaW"
                     /locus_tag="ACHKW5_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7510861.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3785..4921
                     /gene="yiaV"
                     /locus_tag="ACHKW5_001442"
     CDS             3785..4921
                     /gene="yiaV"
                     /locus_tag="ACHKW5_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7510862.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4918..5892)
                     /gene="yiaU"
                     /locus_tag="ACHKW5_001443"
     CDS             complement(4918..5892)
                     /gene="yiaU"
                     /locus_tag="ACHKW5_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7510863.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            6016..6756
                     /gene="yiaT"
                     /locus_tag="ACHKW5_001444"
     CDS             6016..6756
                     /gene="yiaT"
                     /locus_tag="ACHKW5_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO7510864.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQVDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(6703..6966)
                     /locus_tag="ACHKW5_001445"
                     /pseudo
     CDS             complement(6703..6966)
                     /locus_tag="ACHKW5_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6970..7071
                     /locus_tag="ACHKW5_001446"
                     /pseudo
     CDS             6970..7071
                     /locus_tag="ACHKW5_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(7103..7798)
                     /gene="araD"
                     /locus_tag="ACHKW5_001447"
     CDS             complement(7103..7798)
                     /gene="araD"
                     /locus_tag="ACHKW5_001447"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7510865.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPVVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(7792..8652)
                     /gene="sgbU"
                     /locus_tag="ACHKW5_001448"
     CDS             complement(7792..8652)
                     /gene="sgbU"
                     /locus_tag="ACHKW5_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO7510866.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEMMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(8645..9307)
                     /gene="ulaD"
                     /locus_tag="ACHKW5_001449"
     CDS             complement(8645..9307)
                     /gene="ulaD"
                     /locus_tag="ACHKW5_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7510867.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(9304..10800)
                     /gene="lyxK"
                     /locus_tag="ACHKW5_001450"
     CDS             complement(9304..10800)
                     /gene="lyxK"
                     /locus_tag="ACHKW5_001450"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO7510868.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGGVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(10804..11790)
                     /gene="yiaO"
                     /locus_tag="ACHKW5_001451"
     CDS             complement(10804..11790)
                     /gene="yiaO"
                     /locus_tag="ACHKW5_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO7510869.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFLQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            complement(11803..13080)
                     /gene="yiaN"
                     /locus_tag="ACHKW5_001452"
     CDS             complement(11803..13080)
                     /gene="yiaN"
                     /locus_tag="ACHKW5_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO7510870.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(13083..13556)
                     /gene="yiaM"
                     /locus_tag="ACHKW5_001453"
     CDS             complement(13083..13556)
                     /gene="yiaM"
                     /locus_tag="ACHKW5_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO7510871.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(13674..14141)
                     /gene="yiaL"
                     /locus_tag="ACHKW5_001454"
     CDS             complement(13674..14141)
                     /gene="yiaL"
                     /locus_tag="ACHKW5_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO7510872.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            complement(14153..15151)
                     /gene="yiaK"
                     /locus_tag="ACHKW5_001455"
     CDS             complement(14153..15151)
                     /gene="yiaK"
                     /locus_tag="ACHKW5_001455"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO7510873.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15352..16200
                     /gene="yiaJ"
                     /locus_tag="ACHKW5_001456"
     CDS             15352..16200
                     /gene="yiaJ"
                     /locus_tag="ACHKW5_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO7510874.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            16302..16775
                     /gene="ysaA"
                     /locus_tag="ACHKW5_001457"
     CDS             16302..16775
                     /gene="ysaA"
                     /locus_tag="ACHKW5_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7510875.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(16927..18180)
                     /gene="avtA"
                     /locus_tag="ACHKW5_001458"
     CDS             complement(16927..18180)
                     /gene="avtA"
                     /locus_tag="ACHKW5_001458"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO7510876.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18358..20388)
                     /gene="malS"
                     /locus_tag="ACHKW5_001459"
     CDS             complement(18358..20388)
                     /gene="malS"
                     /locus_tag="ACHKW5_001459"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7510877.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            20708..21532
                     /gene="bax"
                     /locus_tag="ACHKW5_001460"
     CDS             20708..21532
                     /gene="bax"
                     /locus_tag="ACHKW5_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO7510878.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(21640..22818)
                     /gene="xylR"
                     /locus_tag="ACHKW5_001461"
     CDS             complement(21640..22818)
                     /gene="xylR"
                     /locus_tag="ACHKW5_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO7510879.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEIML
                     "
     gene            complement(22896..24077)
                     /gene="xylH"
                     /locus_tag="ACHKW5_001462"
     CDS             complement(22896..24077)
                     /gene="xylH"
                     /locus_tag="ACHKW5_001462"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO7510880.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(24055..25596)
                     /gene="xylG"
                     /locus_tag="ACHKW5_001463"
     CDS             complement(24055..25596)
                     /gene="xylG"
                     /locus_tag="ACHKW5_001463"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO7510881.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(25674..26666)
                     /gene="xylF"
                     /locus_tag="ACHKW5_001464"
     CDS             complement(25674..26666)
                     /gene="xylF"
                     /locus_tag="ACHKW5_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7510882.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            27032..28354
                     /gene="xylA"
                     /locus_tag="ACHKW5_001465"
     CDS             27032..28354
                     /gene="xylA"
                     /locus_tag="ACHKW5_001465"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO7510883.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            28426..29880
                     /gene="xylB"
                     /locus_tag="ACHKW5_001466"
     CDS             28426..29880
                     /gene="xylB"
                     /locus_tag="ACHKW5_001466"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO7510884.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            30049..30390
                     /gene="yiaB"
                     /locus_tag="ACHKW5_001467"
     CDS             30049..30390
                     /gene="yiaB"
                     /locus_tag="ACHKW5_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO7510885.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            30436..30873
                     /gene="yiaA"
                     /locus_tag="ACHKW5_001468"
     CDS             30436..30873
                     /gene="yiaA"
                     /locus_tag="ACHKW5_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO7510886.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(30915..31910)
                     /gene="wecH"
                     /locus_tag="ACHKW5_001469"
     CDS             complement(30915..31910)
                     /gene="wecH"
                     /locus_tag="ACHKW5_001469"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO7510887.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            32085..32384
                     /gene="ysaB"
                     /locus_tag="ACHKW5_001470"
     CDS             32085..32384
                     /gene="ysaB"
                     /locus_tag="ACHKW5_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO7510888.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            32479..33390
                     /gene="glyQ"
                     /locus_tag="ACHKW5_001471"
     CDS             32479..33390
                     /gene="glyQ"
                     /locus_tag="ACHKW5_001471"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO7510889.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            33400..35469
                     /gene="glyS"
                     /locus_tag="ACHKW5_001472"
     CDS             33400..35469
                     /gene="glyS"
                     /locus_tag="ACHKW5_001472"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO7510890.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            35795..35947
                     /gene="hokA"
                     /locus_tag="ACHKW5_001473"
     CDS             35795..35947
                     /gene="hokA"
                     /locus_tag="ACHKW5_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO7510891.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(35936..35995)
                     /locus_tag="ACHKW5_001474"
     CDS             complement(35936..35995)
                     /locus_tag="ACHKW5_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510892.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(36135..36347)
                     /gene="cspA"
                     /locus_tag="ACHKW5_001475"
     CDS             complement(36135..36347)
                     /gene="cspA"
                     /locus_tag="ACHKW5_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO7510893.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(36628..36918)
                     /gene="yiaG"
                     /locus_tag="ACHKW5_001476"
     CDS             complement(36628..36918)
                     /gene="yiaG"
                     /locus_tag="ACHKW5_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO7510894.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            37352..38062
                     /gene="yiaF"
                     /locus_tag="ACHKW5_001477"
     CDS             37352..38062
                     /gene="yiaF"
                     /locus_tag="ACHKW5_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO7510895.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(38112..39086)
                     /gene="ghrB"
                     /locus_tag="ACHKW5_001478"
     CDS             complement(38112..39086)
                     /gene="ghrB"
                     /locus_tag="ACHKW5_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO7510896.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(39190..39849)
                     /gene="yiaD"
                     /locus_tag="ACHKW5_001479"
     CDS             complement(39190..39849)
                     /gene="yiaD"
                     /locus_tag="ACHKW5_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO7510897.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            40002..42335
                     /gene="bisC"
                     /locus_tag="ACHKW5_001480"
     CDS             40002..42335
                     /gene="bisC"
                     /locus_tag="ACHKW5_001480"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO7510898.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(42304..42744)
                     /gene="yiaC"
                     /locus_tag="ACHKW5_001481"
     CDS             complement(42304..42744)
                     /gene="yiaC"
                     /locus_tag="ACHKW5_001481"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7510899.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(42741..43304)
                     /gene="tag"
                     /locus_tag="ACHKW5_001482"
     CDS             complement(42741..43304)
                     /gene="tag"
                     /locus_tag="ACHKW5_001482"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO7510900.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            43462..44160
                     /gene="yhjY"
                     /locus_tag="ACHKW5_001483"
     CDS             43462..44160
                     /gene="yhjY"
                     /locus_tag="ACHKW5_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO7510901.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            44389..45591
                     /gene="yhjX"
                     /locus_tag="ACHKW5_001484"
     CDS             44389..45591
                     /gene="yhjX"
                     /locus_tag="ACHKW5_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7510902.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT        11167 a        11297 c        11753 g        11446 t
ORIGIN      
        1 cgaatgaaaa tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg
       61 catcttcaac tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg
      121 caatgaatat gatggcagat tatggattta cccgtacctt aattgtcact gacagtatgt
      181 taacgaaatt aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta
      241 gcgttattta tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga
      301 aattacttaa agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg
      361 actgcgcaaa aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag
      421 gcgttggccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta
      481 cggcctctga aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg
      541 cgattgttga taaacatgtc actccgctgc tttctgtcaa tgactcctct ctgatgattg
      601 gtatgccgaa gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag
      661 cgtatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca
      721 tgattgccga aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag
      781 caatggctta tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg
      841 ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg
      901 ccgttttgct gccgcatgtt caggtattca acagcaaagt cgccgccgca cgtctgcgtg
      961 actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa cgatgcggaa ggtgctgaag
     1021 cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg
     1081 acctgaacgt gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct
     1141 gtggttttac taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag
     1201 cgatgtaatc atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc
     1261 tgtaaccctt gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga
     1321 tttcgccagt cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa
     1381 taatccccct tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc
     1441 ccgctatgac aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca
     1501 gaatctgacg ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga
     1561 catcgatctg gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt
     1621 gcaggatcgc gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct
     1681 gttagcgaca gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga
     1741 tgtaccgctg gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg
     1801 tgggatcagt gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt
     1861 ggtggggcag attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc
     1921 cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt
     1981 actgctgcta atggaaatcg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa
     2041 cggcgcaggt ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc
     2101 gtttaccggc tcaacggaag tgggccaaca aattatgcaa tacgccacgc aaaacattat
     2161 tccggtgacg ctggagctgg gcggcaaatc gccaaatatc ttctttgctg atgtgatgga
     2221 tgaagaagat gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca
     2281 gggtgaagtt tgcacctgtc cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt
     2341 tatggaacgc gccatccgcc gtgtcgaaag cattcgcagc ggtaacccac tcgacagcgt
     2401 gacgcaaatg ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga
     2461 tatcggtaaa aaagagggcg cagacgtgct caccggcggg cggcgcaagc tgctggaagg
     2521 tgaactgaaa gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg
     2581 ggtgttccag gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga
     2641 agaagcgctg gagctggcga acgatacgca atatggcctg ggcgcaggcg tctggagtcg
     2701 taacggtaat ctggcctata agatggggcg cggcattcag gccgggcgcg tgtggaccaa
     2761 ctgctatcac gcttacccgg cacatgctgc gtttggtggc tacaaacaat caggtatcgg
     2821 tcgcgaaacc cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag
     2881 ctactcggat aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca
     2941 ttgcctgatg cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga
     3001 taaggcgtac acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta
     3061 aacctacgtg cgcaataacc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt
     3121 gtcaggagtt taatctcgcg tttcgatggt gagcgggcga atcaggccgt aaatcaccat
     3181 tggtgatccg aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat
     3241 ctgtactgtg gatgttttac tggagttatt cattagtttg gccaatagtt ccattatttc
     3301 aatatgataa gtctcgacct gttgaaaagc caggtagcac acttcagaaa gtatataaaa
     3361 aacaatatgt cgttatataa ataaggaaat tgcactgcat catggcattt cataatatgc
     3421 gttaattgtt cttttttata ttctctgagg gaaatatgtt cctggactat tttgcactgg
     3481 gagtgcttat ttttgtattt ctggtgatct tctatgggat tattatttta catgatattc
     3541 cctacctgat tgctaaaaaa cgtaatcatc ctcatgccga cgccattcat gttgctggtt
     3601 gggtgagtct ttttacgtta catgttatct ggccattttt gtggatttgg gccacgcttt
     3661 accgcccgga gcggggatgg ggaatgcaaa accatgactc ctccgttgtg cagctacaac
     3721 agcgtattgc cgggctggag aaacagctcg ccgacatcaa atcctcttct gccgagtaat
     3781 acttatggat ttactgatta ttttgaccta tgtggctttc gcatgggcaa tgtttaagat
     3841 cttcaaaatt cctgtaaata aatggaccat tcccacagcg gccctgggtg gcatatttat
     3901 tgtcagtggt ttaattctgt taatgaacta taaccatccg tataccttca aagcgcaaaa
     3961 agcggttatt tctattcctg ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga
     4021 taagaaaaat acgctgatta aaaaaggtga ggtgctattt cgactggacc cgacgcgtta
     4081 tcaggcgcgg gtggatcggc tgatggcgga tatcgttacc gcagaacata aacagcgggc
     4141 gttgggcgca gagttagatg agatggcggc gaatactcag caggcaaagg ccacgcggga
     4201 taaattcgct aaagagtatc agcgttacgc acgcggcagc caggcgaaag taaacccgtt
     4261 ttcagaacgc gatattgatg tggcgcggca aaattatctg gcgcaggaag cctccgtaaa
     4321 gtcatcggcg gcggaacaaa aacagatcca gagccagctg gatagcctgg tgttgggtga
     4381 acattctcaa atcgccagcc tgaaagcgca gctcgcggaa gcaaaatata accttgagca
     4441 gacgatagtg cgtgcgccga gcgatggtta tgtgacccag gtgctgattc gtccgggtac
     4501 ctatgccgcg tcgctgccgc tacgtccggt gatggtgttt atacccgatc agaaacgaca
     4561 aattgtggcg cagttccgtc agaactcctt gctgcgactg gcccctggcg atgatgcgga
     4621 agtggtattt aacgctctgc caggtaaggt attcagcggc aagctggcag ccattagtcc
     4681 agctgttccc ggcggagctt atcagtcgac cggcacctta cagacgttaa acacagcgcc
     4741 gggttcagat ggcgttatcg cgaccattga actggatgag cacactgatt tgagcgcttt
     4801 accagacggt atttacgccc aggtggcggt ctactctgat catttcagcc atgtctcggt
     4861 gatgcgcaaa gtgctgttac gcatgaccag ttgggtgcat tacctttatc tcgatcatta
     4921 atcttcgctt tccgccgctt gttcaggccg cagttgctgc gactgagcaa gctggtggcg
     4981 aatttcacgc agcatttcat ccagcacata ctggacccgc cagggctggt attcccgttt
     5041 acggaagata gccaccagat ccagccacca ttcatacggg tgctgaaagc agggcaccag
     5101 cgtgccgtct tgcaggtcgc tctgcacact tttgtctggc gcaaacacaa tccccaggtg
     5161 atttcgtgcc agctccagcg ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg
     5221 ataatcccgc acctctttac tcccggcgac gttgaagcgc cagatattcg cgtcgtcaat
     5281 catcatcgag tcgatcaaaa tacaggagtg atgttcgagt tcgtcaggac ggctaatagg
     5341 atgtttctca agatagcgct ggctggcata agcggttacg gcatattggg taataaaact
     5401 ggcaaccagc gattcatctt ttggtggagc gtagctgatt aaaacatcac aatcatcagg
     5461 aaattcgacg ccttcataaa aggcattacg ctcaagattg caggttttta gcgataaggt
     5521 gatatcaccg atatctttaa ttttatcgat tacatgtttg gataaatagg taattatgcc
     5581 ggttggggcg tagatagtga cccgaccacg tttctcatgc ttataatctg caataaaatt
     5641 attaagttgc tcgtttctat ccagcatgtc gttgatgtac ggtaacagcg cggtgccaaa
     5701 aggtgtgagc atcagctgcc gggtggttcg gtcaaagact tttaaaccca cttttgattc
     5761 aaaatcagca agatatttgc tgacgttggc ctgtgcgatg ccaagtacag tcgcggcatg
     5821 gctgatattt tcactggcag cgattaccga gataattttt aactcccggt acttaagttg
     5881 taattttgtc atcgcaacag cccaattata tataatttta tatattacta tataaatatg
     5941 aatcttgcac cggagaaatt gtaagcatta ctatgccgac tcttttttat cttattttaa
     6001 aatggaatac cggacatgtt aattaaccgc aatattgtgg cgttatttgc gttgcctttt
     6061 atggcaagcg caactgcttc tgaattatcc attggtgccg gtgcggctta taatgaatcg
     6121 ccttatcgcg gttataatga aaatacaaag gcaattcccc tgattagtta tgaaggtgat
     6181 tctttttatg ttcgtcagac cacgttaggt tttattctgt cgcaaagtga aaaaaatgaa
     6241 cttagcctga ccgcatcctg gatgccgctg gaatttgacc ctgccgataa tgacgatcat
     6301 gccatgcaac aagttgataa gcgtgatagt acggctatgg cgggggttgc ctggtatcac
     6361 cacgagcgtt ggggaaccgt gaaagcctct gcagctgcgg acgttctgga taacagcaac
     6421 ggctgggtgg gggagctatc ggttttccac aaaatgcaga taggtcgcct gtcgctgaca
     6481 cctgcgctgg gcgttctcta ttatgacgag aatttcagtg actattacta tggcatttca
     6541 gagagtgagt cccgtcgtag cggtctggca agttattccg cgcaggatgc ctgggtgccc
     6601 tatgtcagcc tgacggcaaa atacccgata ggagagcacg tagtattgat ggcgagcgcc
     6661 ggatacagcg agctgccgga agagattacc gacagcccga tgattgatcg taatgagagt
     6721 tttacctttg tcaccggcgt gagctggcgt ttttaattca ccggtggatg tcggtgcggc
     6781 tggtgctccg tcgtaatgcc acaaaggttg ttgctctgga ttgctctgtt cataccggga
     6841 acctggcgtc tgaatcagga tcttatggct gaacaatccc ttgccttcaa atgtcgtaaa
     6901 tggcgcatcg gcgggcagca acaggttatg cagtgactcg acgttgtcgg cctgctccat
     6961 gcaatacggt ttaatgaggt tggctatttc cggcagattt ttcgcaagca caccggttta
     7021 acgccagcag cgtggaagcg gcgttacagc aaggaacata tcaattcgta gtgccggggc
     7081 gatgaagccc cggcgtgagg gattactgcc cgtaataggc attggcccca tgcttacgca
     7141 ggtagtgctt atccagcagt tcgttttgca tcgcagggag ctgcggcgca agctggcgcg
     7201 agaataggcc catataggcg cattcttcga gtactacggc gttatgcacg gcatcggcgg
     7261 cgtttttacc ccatgcgaac gggccgtgag aatgcaccag caccaccggg atttgtgccg
     7321 gactcctgcc acgttcttcg aaggtttcaa tgatcacttc gccggtctga tattcatatt
     7381 cgccgttaat ctcctctgcg gtcatctgtc gcgtgcaggg gatggcacca tagaaataat
     7441 cagcgtgcgt ggtgccccag gcggggagat ccagcccggc ctgcgaccag atggtggcgt
     7501 ggcgtgagtg ggtatgcaca atgccaccga tttcggcgta gcgacggtaa agcgccagat
     7561 gcgttggcgt gtcggacgag ggctttttgc taccttccac caccttgccg ctggctatct
     7621 caaccaccac catatcgtcg gcggtcatca cgtcatactc gacgccggaa ggtttgatta
     7681 ccatccattg ccgcgtttcg tctaccgcgc tgacattgcc ccaggtgaac gtcaccagat
     7741 ggtgagcggg aagcgccaga ttcgccgcca gcacgtcggc tttcagttgc tctaacatat
     7801 aaatccagcc tcctgcatac gcgcttcaat ccaacgccgc gcctgaataa tctccagcac
     7861 cggctctttg gctttttcgg tccacatctc aatcaggaaa gaaccgcgat agttcagttt
     7921 atgcagcgtt ttgaagatgc caacgaaatc gacgcagcct tcgccaaacg gcacgtcgcg
     7981 gaactgtccg ggactttgct cggtaactgg ctgggtgtct ttcaggtgga tcgcggcgat
     8041 acggtcaatg cccagtttca gttcggcggg aacatcattg ccccaggcgc tgaggttgcc
     8101 gacgtccggg tagacggtga accacggcga ggcgagcatc tcgtcccact ttttccattt
     8161 actgatggag ttcataaacg cggtgtccat catctccacc gctagcatga cttgtgatgc
     8221 cgccgcctgt tcgaccgccc acgccagccc ttcagcaaaa cgttgccggg tgccttcatc
     8281 gtggtcttcg taatagacgt cgtagcccgc cagctggatg gtgcgtatgc cgagatcgcg
     8341 tgccagacga atcgctttgc tcatgatctc ccgtgctcgc tggcgtaccg cgtcgtcacg
     8401 gctaccaaaa ggaaagcgac gatgtgcgga caggcacatc gacgggatcc caacacccgt
     8461 ttcgatcatc gcggcaacca gcgaagtcct ttgtgcggtg ctccagtcaa gacgtgaaag
     8521 ccgttcgtcg gtttcatcca ccgacatttc gacaaaatca aaaccgcagc tttttgccag
     8581 caccagccgc tccggccagg agagatcttt cgccagcgcc ttttcataaa tccctaacgg
     8641 atgattacgc acgcttgcct ccccagatag cgtcgatttg cgcatggaaa tcgccagcca
     8701 cctgcgccgg atttgctgag cctgccagtg ctcgcccggc aataaacgct ttcacgcgaa
     8761 tatctttaaa cagcggcagg tcagcagggg taatcccgcc ggtaatggaa agctcaaggc
     8821 cgatatcgga aagcgccttc atgcgtgcca gatcggcttc gccccactgt tgcccgctgg
     8881 cctgtgcatc acgaccgcga tgataaatcg cctgccgcac gccaatacga tgccagtcgc
     8941 gggcgtcgtc cagcgtccag ttaccgaaca gctcgatctg aatttcacca ccgcagcgtt
     9001 gcgccatcgc gtggcctttt tccaccgtcg cgagcggtgc ggcgcagatg atggtcatcc
     9061 agttagcgcc tgcgccaaat gcctgttgcg cgagcgtttc accagcgtcg gcgaccttcc
     9121 agtcggcaac gatgattttg tcagggcact gttcgcgcaa ggctttcacc gcgccaagcc
     9181 cttcgtttaa acagagaatg gtgcccgctt cgacaatatc gacgctgtct tttaactgcg
     9241 tcacgtcgcg ctgtgcggct tcaagtgatg agtggtcgag tgccagttgc agaagtggtc
     9301 ggctcataat atgtgctcct tgatgcgggc gtgaaagccc tgaagtgcgg caatgagatg
     9361 ctgatagcgt tggtattttt gttggtaaag ctgatgggcg gtcatatccg gcagcagggt
     9421 gcgtaccggg tgctgtaagt cacgttgagc ttcgctgaaa tcgcgataaa ctccggtgcc
     9481 gacacgggcg gcaagtgccg cgccaaaaca gccggtttct tccacctgcg gcagctcgat
     9541 acgcagaccg ctgacgtccg ccagcatttg catccagaca tcggagtgcg ccgggccgcc
     9601 agtgacgcgc agggtgtgca catcagtaaa acgttcgcgc atccggttga ggtgggtcat
     9661 atggctgaac accccccctt cataaatggc ctgcaacagg tgtgcgcggg tgtgaatggc
     9721 ctgcatcccg tagaaaccgc tggtcatctc gagtccggcg ttgctgccgt acaggaacgg
     9781 caggaaaaag agatcgccac cggccttcgg caagctggca acggcctggt tgatctcatc
     9841 aaacgagatt tctccccact gtgcggtaaa ccattcgagg ttgccggaag aggtggggct
     9901 ggcttcgtga acgataaatt caccatcgtt aacgtagcga ccatagacat acggatgcgc
     9961 ttcaccgtca cgtaaaccgc gggttatgcc gctggtcacc gcccaggtgc ccatcaccgc
    10021 attaagggta aattcgtctt cgatcccggc gcagagtgcg gtggaaacca catcaaacag
    10081 gccgccgaca acgggcgtac ccgctttcag accggtcagt acggctgtct gagcggtgat
    10141 ctccccgcag atttcggcag atccgacaac aggcggcagg gcgtgattga tttcagcaat
    10201 ccccagccag tcggtgaggc acgggtcata ttccccaaga ctcatgttgt agagattgga
    10261 ctcggaaata ttgctctctt cacagccttt gacgccggtt aaacaccagc gcaggtagtc
    10321 gtgcgtcatc atcacgcagc caatttgcgc gtagcgttcc ggttcgtgct ctttaagcca
    10381 acgtaacagc gataccggat gcccggtcca caaggtttgt cgggtcagcg ggtagagttt
    10441 ttccgggatg ccatcttcct gccagcgacg aacgatttcc atcgcccggc gatccgagga
    10501 taaaatggcg ttaccgagcg gcttgttgtt tttatccagc aaaaacaagc cttttccttg
    10561 tgcggagata ccgataccga caatttgttc cccgctaacg ccggaatggg taagcagttc
    10621 gcgaatgaca gccatgcagc attgccacag ttctgccata tcgcgctctg cccaacctgg
    10681 ctgcgggctt aatgcgcaca gcggcaggcg ctgcacgcct gcctcccggc cttcgcggtc
    10741 atacagcccg gctttcagcc agctaccgcc acaatctaac cccagccagt attgcgtcat
    10801 ggtttattgc acctcatcca cggctttcag tagatcagca ccctgcggaa catcttttac
    10861 aaacatgtcg cgaacctgat tacccagagc gtcgctaaag gctttgcggt cgagatcggt
    10921 gatgacttcc acgcccgctt ctttcatgcc gtcgatgatt ttttgctgat cttcggcaac
    10981 cagtttgcgc tggtagttac ccgcttcctg cgcagatgaa attagcgctt gcaggaactc
    11041 cgggcttaag ccatcaaact tcgctttgtt gatcaccacc agaagcgggg aataggcgtg
    11101 gtgcgtcaga gaaaggtact tctgcacttc gaaaaatttt gctgaccaga cgacgttgat
    11161 cgggtgttcc tgagcgtcga tagtgcgggt ttccagcccg gtatagactt cggcaaacgg
    11221 catcgggatc gggttagcgc caaagacttt gaatgcggcg atattcatcg ggctattgtt
    11281 ggtgcgaatt ttcagccctt tcaggtcggc gggggtttta accggtgcgc gcgagttggt
    11341 gacatcgcgc cagccgtttt cccagtaggc cagtactttt agtcctttac cttcaagtga
    11401 ggctttcaga tcatcaccga ctttgccgtc gagcgtttta tgcgcgtgag cggtatcgcg
    11461 gaacaggaaa gggacatcaa gcaagttcat cactggtgat aacccggcaa agttattcga
    11521 gccggacatt tccatatcga tggtgccgcc acgtacgccg ctgatcatcg cctgcgcgtt
    11581 accgagggtg ctatccggaa acaattttaa tttcagctcg cctttggttc tctcctgcaa
    11641 taaatcattg aattttttcg ccgcaatatg ttgcgagtcg gtttgtgagg tttcataacc
    11701 gaaacgtaaa gattgcgccg ccagagaaga tgtgctgaat gcagccaggc cagcaatgaa
    11761 taatgcgtag gttacagagc gtaatttcat aatattttcc ttttaattaa tccatttcaa
    11821 agggaggatg atgagatcgg gaataaaaac aaatacgact aataatgaat ataaaaccag
    11881 gacgtaaggg aaaacgcctc tgactgcatc atcgaatttg agttttgcca cccctgaaat
    11941 aacgttaagt acattaccga taggcggtgt aattaagccg attgagcagt taatgataaa
    12001 catgacaccg aaataaatcg gatcgattcc tgcctcttta actaaaggca ttaataccgg
    12061 ggtaagaatt aataccgtcg gcgttaaatc catgaccatg ccgacaatca ggatagccac
    12121 cataatgacg ataaacagca gacgcggtga atcgaccaac ggttgcagta aatcagaaac
    12181 catcatcggc agttcagcaa tggtaatcag ccaggcggaa acttgtgccg aggccaccag
    12241 aaacataacc actgaggtgg ttttcgccgc gccaatgagt acatgccaaa gtgtggcgaa
    12301 ggtcatttca cggtaaataa ctgtggcgac aaacagcgca tagaaagcag caacggcccc
    12361 ggcttcggta ggggtaaaca gaccggagcg aaacccgcca ataatgatga ctggaagaaa
    12421 cagcgcccag ataccggaga caaaagagtg ccagatttcc tgcatcgttg ctttttgctg
    12481 gcggggtaaa ttaaggcgac tggcctgcca ccaccaggta agcatcagcg ttgcgcccat
    12541 catcatgcca ggggcaatgc ccgccataaa cagcttactg atggataatc cgctggaaac
    12601 gccgaagata ataaacggaa tggaaggggg aataattggc gcgataatgc cgccagaggc
    12661 aatcagcccc gccgcccggt tgaccgggta gttggcgctg cgcatcatcg gcaccagtag
    12721 agcggcaacg gcggcggtat ccgcaacggc agaaccagaa aggctcgcca taatcatagc
    12781 tgccagcacg ccgacgtagc ccagcccgcc tggtttatgc cccaccagtt tcatcggcag
    12841 gtcaacaatg cgttttgaca agccgcccgc attcatgatt tcacccgcca gcacaaagaa
    12901 cggaatcgcc agcagggaga agctatcggc tccgttcacc agcgtttgtg ccatgatctg
    12961 gacatcaaac atgtccagcc agaacattaa cgccgccccg cacaacaaca gtgcccaggc
    13021 aataggcaaa ccgatagcaa taccacccaa cagacagccc agaaaaatca gcacagccat
    13081 gattaagctc cttgcggtgg cgacgttaga gaattgctac gcgtgatgag ttgatataaa
    13141 tgacgcagtt caaagaatgc gataacgagg ctggtgggca gacaagcggc atacatcagg
    13201 ccgatgggta aaccgaggat cggtgaataa tcgctccagt cctgaattgt ttttagcgtt
    13261 gcgccccagg ccagtgcgcc acaaataaat aagattaagg aatgggtaac cagagcgact
    13321 cgtcgttgcc atgcggggga gagtttctcc accagaaagg tgacctgaac gtgggcgtta
    13381 tccataaaag ccacaatcgc gccaataaat gttaaccaga caaataaata acgtgacaat
    13441 tcatctacag ataaaatgct tgtctgaaaa ccatatctta aaataatgtt tataaacaca
    13501 atacaggaaa gtacggcgag attaatcgcc agtattgctt caagtatttt tttcatagct
    13561 attccttgag gctatgtctg tcataattca agttgcatgt gcagcaacct aaattattaa
    13621 tatagttaga gcaataacat tacccagtcg ttctggcgta gacgattatt cgattaattc
    13681 agcgccgtta atgcgacttt aaccacaatt tttcgtatct cagaggctgt ttgcaaaata
    13741 caacctggtc ggtgaacatc ctgcggaaag aaaattgcgt agctgcccgg tatcatttct
    13801 ataaatgatt catgttcact gtcatgataa aaaataatat cgcgctgctc taatagtgat
    13861 tcgctgactt tattatttcc cgtatcaata gcaatgccga ttttctcttc accccacgcc
    13921 agaaactgaa tatcgatata ccgacgatgc acttccggac ggttttccac cgcttcgcgt
    13981 gtggttaaat cgataatttg cgcataaata tttttgccgt cgatttcgac aacgcccggc
    14041 tccagggcgt tgaaatcggt agcgcgcaga aaatcgagcg ccttttcaat ggcggcgggc
    14101 aaacggcacg gattgggctg cgcgatatgt ccaaaaatca tgacttatct ccttataacg
    14161 cctggatttt ggcccacacg ctgtcatcaa cggtaatacc gttacggcgg ttttcggcca
    14221 gcagggtagt aaattcgtgg ccgggtaagc ggatggcctg attttcgtca gcgcgttcag
    14281 cggtagtaac gtaatccatg atacgttgca gcttggcatc gcgggtggga ccgtcgataa
    14341 gcttgtccac ttcaatggca ataaaaattt gtgaaatgcc gtattcgtcg ctgttgtcct
    14401 gggtgacttc ggcaaccgat gcgccgtcgg aaaggagagt agcgatcata tccagcacaa
    14461 tcgacatgcc agaacctttc cagtagccca tcggcaaaat gcggcgattc ttctcgataa
    14521 cgccaggttc tttggtcaaa ttgccctcat catcaaagcc accatcgacc gggagctgac
    14581 ggcccgccag gcggttaact tctaacatgc cgtaagagaa catcgacatc gacatatcga
    14641 ccatggtgat cggcgtggaa ggaatggcga cgatcagcgg gttagtgcca atgcgacact
    14701 cttttgcgcc ccacggcggc attacggcga tggagttggt ccagcaaatg ccaatatagc
    14761 ctttttccgc cgcctgccag ccgtagctgc cgccgcgcat ccagtggttg gcattacgaa
    14821 gtgccaccag accaataccg tgatcggcag ccagttcaat ggcgcgatcc atcatctttt
    14881 tcgccgtcag gttgccgatc gaacgctggg catcccactg ttcaattgcg ccgaggctgg
    14941 ttatacgttt gggttgggca tcaggaatga tatcgccgtt ttccagttgt tgaatgaaac
    15001 gagggaaacg attaacgccg tgagaataaa cgccggattc ggtggtgcgg gcgaacatct
    15061 ctgcacaggc gtcagccgtt tcgctgtcaa cgccgcgtga aattaagacc cgattaaagg
    15121 ctgcttttaa ctgctcaaat gtcactttca tcccaggctt ccttgttttt ttgatcgctt
    15181 ttttggctta aaatttcaat atgagaaact tgatttcaaa tatatcgatc gctttttaac
    15241 aaagcaatct gattgatgaa tttcaaaaga cataaaaatc aagtggttat aaattatctg
    15301 tcgagatcgt gaactacggc acactttgcg ctaccatcag gacgcgacaa aatggggaaa
    15361 gaagtgatgg gaaaaaaaga gaacgagatg gcgcaggaaa aagagcgtcc agccggaagc
    15421 cagagtctgt ttcgcgggtt gatgctgatt gagattttga gcaactatcc aaacggttgt
    15481 ccgttggcgc atctttcgga gctggctggt ttaaataaga gtaccgtcca tcgcttattg
    15541 cagggattac agtcctgcgg ctatgtgacc accgcgcccg ccgcagggag ttaccgcctg
    15601 actaccaaat ttattgccgt cgggcagaag gcgctgtctt cgctgaatat cattcatatc
    15661 gccgctccgc atcttgaggc actgaacatc gccactggtg aaaccattaa cttctccagc
    15721 cgcgaagacg atcacgctat tttgatttat aagctggaac ccacaaccgg gatgctgcga
    15781 acccgtgcct atattggcca gcatatgccg ctctactgtt ccgcaatggg caagatctac
    15841 atggcgtttg gtcacccgga ctacgtgaag tcatactggg aaaaccatca gcatgagatc
    15901 cagccgttaa cccgcaatac cattaccgag ctgcccgcga tgttcgacga actggcgcac
    15961 attcgtgaaa gcggagcggc gatggacaga gaagaaaacg aactcggcgt ctcctgtatt
    16021 gctgttccgg tgtttaatat tcatgggcgg gtgccgtacg ccgtgtcgat ttcgctttcg
    16081 acatcacgtc tgaaacaggt gggggagaaa aatctcctga aaccactgcg tgaaaccgcg
    16141 caggctattt ctaatgaact gggatttact gtcagggacg accagggcgc gatcacataa
    16201 cgctttggac aagtgccaaa actttaacat ttccttcgtt ggatcaaagc agtagggacg
    16261 cgctctctgg cactctgctg ttttagtgca aaggagtgat catgaaccgg tttattattg
    16321 cggatgcgac gaaatgtatc ggttgccgta cctgtgaagt ggcttgcgca gtgtcgcatc
    16381 aggagaatca ggattgcgct gcgttgtcac cagacgagtt tatttcccgt attcgtgtca
    16441 ttaaagacca cagctggacc acggcagtag cctgtcatca gtgtgaagat gcaccgtgcg
    16501 cgaatgtctg ccctgttaac gcgataagcc gcgaacatgg gcatattttc gttgaacaaa
    16561 cacgttgcat tggctgtaaa agctgtatgc tggcttgccc gtttggtgcg atggaggtcg
    16621 tttcttcgcg caaaaaggcg agggcgatta agtgcgatct gtgctggcat cgggagacgg
    16681 ggccggcctg tgttgaagcc tgcccgacaa aggcgttgca gtgcatggat gtcgagaaag
    16741 tgcagcggca tcggctacgg cagcagcctg tttgaaacgt ttttccggcg tttctcaacg
    16801 ccggaatagt gcgctttgtt tatgccagat gcggcgtaaa cgccttatcc ggcccacaaa
    16861 ttcttgcaaa ttcaatatat tgcagaaatc atgtaggcct aataagcgta gcgcatcagg
    16921 caatttttag tgactttcag cccaggctct ttctatctct tccgccagaa tcttcacccc
    16981 cgcctcaatt ttctccggct ctggtacgta gttcatgcgc atacattgat gcgtatgcgg
    17041 ccacggtttg tccagccctg ggaagaagtt gtgccccggc accatcagca cgccgcgtgc
    17101 tttcaggcgc tgatagagct gctcggtcgt aatgggcaaa tccttaaacc aaagccagag
    17161 gaaaatggct ccttctggtt tatgaattag gcagcgatct tccggtaaat agcggcgaat
    17221 gatggcgata gtttcctgaa cacgctggta gtaaaacggt ttgatgactg tttcagacag
    17281 gcgcagcaga tcgttacgct taatcatttc acacatcatc gccggaccaa taccgccagg
    17341 tgccaggctg ataatgccgt tcatattggt gatggcagtg atgatttttt cattggcgat
    17401 gataatgccg cagcgggagc caggcagccc cagcttggaa agactcatgc acagcacgat
    17461 attcggattc catagcgggc gcgcttcact gaagatgata cccgggaacg ggacgccata
    17521 agcgttatca atcaccagcg gaatgccgtg ttgattcgcc agcgcgtcaa gcttcagcaa
    17581 ctcttcgtca gtaatcacat tgcctgttgg attcgtcggc cgggagacgc aaatcatccc
    17641 ggtttcttcg ccaatatgca gatgctcaaa atcgacgtgg tatttaaact ggccttccgg
    17701 cagcagttca atattcggac gcgcagagac aaacagatct tcttccagtc cggcgtcagc
    17761 gtagccaatg tattccggtg ccagcgggaa cagcactttt ttgacccgac catcggcacg
    17821 gcgtccggca aacagattaa ataagtagaa aaacgcgctc tggctgccgt ttgttagtgc
    17881 aatattctgt gcttcgatat cccaacccaa cttctcgcgc agcattccgg caagcagtgt
    17941 gagtagctcc gttttcccct gtggaccgtc gtaattgcac agtgcatcag tcgctttgcc
    18001 actttccagc atgtcggtca gtagcgtctg gaagtagtcc tgcatttccg ggatctgcgc
    18061 cggattaccg ccaccgagca taatcgcgcc aggcgtgcgt aaaccgtcgt tcagatcttc
    18121 catcagcagc gtaatgccgg agtggcgggt aaatttgtca ccaaaaaggg agaatgtcat
    18181 agcgggatat ctgtcgaact ctgtagcaag gaaggtaaca ataacgctac atttgctggg
    18241 gtgcaaatcg gtctgttgga gagtgagcgg tgctttatcc tgcaacgctg attagggctg
    18301 acattttatc ctggttggcg gcttcccgag gtactgacga acgggaagcc ggaaaagtca
    18361 ctgttgccca gcccagatga ccagcacttt atcgtcgcca tgctcacgaa caaagccgta
    18421 gccctgcttc agcgaaagtg tcgtttgttt gcccgcgcca attgcgggat ggcgggcgcg
    18481 gaactggctg attttctgcc agtgcgcgac gttggcggca gatttaccgc taacatcctg
    18541 ccagttcata tccgaacgtg taccttgcag cggatcagaa cctgtaggac cgaacggacg
    18601 cgaggattca tcgccataaa agatttgtac cgcgcctggc gctaatagta ataactctgc
    18661 tgctttgtcg cccccttcac ggaacagacg ggtatcatgc gacgagaggt agctcaacac
    18721 gttgaaaccc tgcaatttct ccgccatttg ctgccaggtc gtatccatct gcgccagaca
    18781 atcgactgct ttcgccgcct gctcctgata atcgaaattg atcatcgcat cgaagccgtg
    18841 gcgatagtag tcactttgca tcacgccgtg gccccaggct tcaccggtca tccagaaagg
    18901 tttgtcatct aatgctttgt cggggttagc ttttttccat tcgcgaagcg cggcgctggc
    18961 ttcggttttc agttgctgcc aggcgggcaa ctcaacatgt ttggcggtat cgacccgaaa
    19021 accatcaatc ccatagtcgc ggacccactg acttaaccag tgggttaagt aatcgcgagg
    19081 tgtaaagcct tcgatgactt tagcgtgagt atccgttttg tttttataga acaccggcag
    19141 accagaagcg gtagttgatt cggttttgat atccggcaaa aaggccagcg acatagtgag
    19201 atcgtcgaat ccaggattgt cgtaatcgcc gatatccgtt ctgatccagt tttttcccca
    19261 ccatttatcc cagcctgttt tgtcgctgaa attaatgtaa tcgttaaagc tatgccaggt
    19321 ttgcccggcg gcaggtttcc agtcgctcca gcgttcaccc agcgtttttt tcacctcgtc
    19381 accagaaaga tataacgcgc caaactgata ctcctgcata tccgccagcg tggcatagcc
    19441 ggtgtggttc atcacgacat caaagagaat acgaataccg cgctgatgtg cgctatcaac
    19501 cagcgtccgt agatcggctt cgttgcccat attggcatca agattcgtcc agtcctgtgt
    19561 gtaataaccg tggtaggcat aatgcgggaa atcgcctttt gtaccgccgc cgacccagcc
    19621 gtgaatttgc tcaaatgggg cgcttatcca taaagcatta acgcccaact gctggaggta
    19681 atccagtttg ttggtcaggc cgcgtaaatc gccgccgtga aaagtgccaa tttccgccat
    19741 accgtcttta tgacgtccgt aactctggtc attactggga tcgccgtttt cgaaacgatc
    19801 tgtcagcaca aagtaaaccg tggcgttatg ccagtcgaaa ggggcggggg cgtcagtttc
    19861 tgcccgttcc agcaggagta aaccgttgct ggtagcagcg ggttgtaacg ttatttgacc
    19921 gttcttcact atcgcaattt gctggctgta ataatcccgt acggcggctc cttccgggaa
    19981 agtggcgctg acatccagcg tgagcggtaa tccatcccat ttcgggcatt cacggaccag
    20041 gtttgctacc ggttcggcgg cgttctggat ggaaatcatc aatgttggcg taccggagcg
    20101 ggtgtctatt tgcagcgtat atttgccgtc tctgaacaat cgccattgag gcggcgtgtt
    20161 gctacaaggt tgcagggaaa gcatctgatt gagttttatc gcatctgcag gctgccagca
    20221 ctgttggtca aaatttagcg tgagtggacg cgtacctttg ggcaactgcg cgtggctgac
    20281 aaatgttccc gttccctgtt cgctaaaggc agggaacccc ggagaagtcc agctggcggc
    20341 aacggcgaag ccaggaagga gtgtcagaaa acaggcggcg agtttcatgg tggatgagtc
    20401 cttattgctg cgatttcaga cagtttgcca gcgataagcc agacaaaact catccctgag
    20461 cggtaagtcc acaggatgag aagcaagggt gagcgatctc gcgcaaaaaa cggctgaatt
    20521 ttgcgataac ccccacattt tctgcgattt agcgccaatc tgaatcgtta acacgtgaca
    20581 tagtttcaga tttggactat tccttggggt gctcttgaca gctatttttg atatgatttg
    20641 agattcaact ctcaaatttg tgaaaaatat aaggtgttgg aatgattaaa tccgaccagg
    20701 agacctgatg attttgactc ccatacgacg atatggggcg atgattctta tgttactcac
    20761 tctggtgttt tcgagtgagg tgttagcgaa gacgcacaca acaacagcga gtcaaaagtc
    20821 ccacttaact aaagctagta ataaacaggt aagcagtaaa caagagtatt ctcgcaatag
    20881 tgcaaagagt agttcacttc ctgatttgcg aaaataccct tccggaacac caaggaaaaa
    20941 ggcgtttctc cggaccgtaa tgccttacat taccagccaa aatgcagcca ttactgcaga
    21001 acgtaactgg ctgatttcaa aacagtatca gggccaatgg tcacctgctg agcgtgcgcg
    21061 tctgaaagac atcgccaaac gctacaaggt gaagtggtct ggtaatacgc gaaaaatccc
    21121 gtggaatacc ttgcttgaac gcgtagacat tatccctacc agtatggtgg cgacgatggc
    21181 tgcagcagaa agcggttggg gaacgtcgaa gctggcgcgc aacaataaca atctgttcgg
    21241 catgaaatgc atgaaaggac gttgtaccaa tgcgccgggt aaagtgaaag ggtactcaca
    21301 gtttagttct gtcaaagaat cggtgagcgc ctatgtcact aacctgaata cgcacccggc
    21361 ttactcttcg ttccgtaaat cgcgtgcgca gctgcgtaaa gcggatcagg aagtgactgc
    21421 cacagcgatg attcacaagc tgaagggcta ctcgaccaaa gggaagagtt ataataacta
    21481 cctgttcgca atgtaccagg ataaccaacg gttaatcgcg gcgcatatgt aattgcattt
    21541 ccttgagcct tatctgattt gtcggttgga taaggctttt gctcgcatca ggtggctgtg
    21601 ctgagttccc tgatgtgacg ccgacaattc tcatcatcgc tacaacatga tctcgctatt
    21661 tacatcgcga tactcttttg gcgttgtgtc atatgctttt ttaaaaacag agtagaaata
    21721 ttgcagcgat ggataaccgc acatttgcga tatctcattg atcgacaagg tggttgaaat
    21781 cagcagactg cgcgctttct ccagcttctc ggcatgaatc atggcatgga tggtttcacc
    21841 cacctcttct ttaaaacgct tctcaagatt ggagcgcgag atcccgaccg catccagtac
    21901 ctgatccact ttaatccctt tacaggcgtg attacgaatg taatgcatgg cctgaataac
    21961 ggcgggatcg gtcagcgagc gataatctgt tgagcgccgt tcaatgacgc gaactggtgg
    22021 gaccaaaatt cgctgtagcg gcatttcttc tttatctaat aatcgatgca acagttttgc
    22081 cgcctgatag cccatttgcc gcgcgccctg agcgaccgaa gaaagggcga cacgcgacag
    22141 atagcgggtc agttcttcgt tatcgatgcc aatcacgcat aatttttccg gtacgggaat
    22201 atgtagatgt tcacatactt gcagaatatg ccgcgcccgg gcgtcagtaa cggcaataat
    22261 cccggtttgc ggtggcagcg tttgtagcca gtctgccagc cgattttgcg catgttgcca
    22321 gttctctggc gcggtttcta acccctgata aaccactccg cgatactttt cttcggcgac
    22381 aagctgacga aatgcatatt cgcgttcagt ggcccaacgt ttgccgcttg attccggaag
    22441 accataaaaa gcaaagcggt taacgccttt ctcttttaaa tgcaaaaatg cgctttcaac
    22501 cagcgcatag ttatcggtgg caatgtaatg aacgggtggg taactttctg caaggtgata
    22561 cgagccgcca accccaacaa tggggacgtc gacatcagcc agcgcttgct cgatctgttt
    22621 gtcgtcgaag tcggcaatga cgccatctcc taaccagtcc ttgattttat caatgcgggc
    22681 gcggaaatct tcttcaatga aaatatccca ttctgattgt gatgcctgta aatattcccc
    22741 tacgccttct actacctgcc ggtcataggc tttattggca ttgaacagta atgtgatgcg
    22801 gtgacgttta gtaaacatgg ttcttttcct gctgaatcat gcaaaaactc aaaaccggta
    22861 atacgtaacc ggctttgaga aaatttttat caaaatcaag aacggcgttt ggttgcggag
    22921 tccatccata ctgccagcaa cagaatcgca cctttaacga tatactgcca gaaggtcggt
    22981 acatccatca tactcatgcc gttatccagt gaagccatga taaatgcccc cattactgct
    23041 ccggcaacgc ttcccacacc gccagccagg ctggtgccgc caatcacgca tgctgcaatt
    23101 gcgtccagtt cggcgatatt tcccgcagaa ggtgaaccag cgccaagtcg agaactaagg
    23161 attaatccgg cgatggctac cattaatccg ttaatcgcga acacggcaag tttggtgcgt
    23221 tcaacgttaa tcccggagag acgtgctgct tccagattgc cgccgatggc ataaatgcgt
    23281 cgtccaaatg ccgtccgcgt tgccataaac attccgccga gtaacagcaa cgtcagcagc
    23341 agaacaggag tgggaacgcc acggtaatca ttcaacagcc agattgcgcc taatacgatg
    23401 atagcggtta aagcctggcg accgactact gcggtagagg ccggagactg caaacccaaa
    23461 gcctgacggc gcattcttcc gcgccattgc caaccaacaa aagccattaa gccaagcgcg
    23521 ccaatgatga agccagtgct ggcaggtaga tagctttgcc caatttgtga catcgcggcg
    23581 ctggtggggg aaacagtcgt gccgttggtg atgccaatga gtatgccgcg aaatgccaac
    23641 atgcccgcga gggtgacaat aaatgaaggg actttgcggt acgcgaccca ccatccgttc
    23701 caggcaccga gaagcagtcc cagaaccaac gtcacaatga tggtaagtgg caaaggccag
    23761 cctaaccaga cgtcacaaat cgccgcgacg ccacctaata gccccatcat tgagccgacg
    23821 gaaaggtcga tttcagcaga aattatgacg aacaccattc ctaccgcgag gatgccggta
    23881 atcgcggtct ggcgtaacag gttggagacg ttacgggcgc ttaagtaggc accatcggtg
    23941 gtccaggtaa agaacagcat gattgcgatg atagctgcaa tcatcacgaa gacctgcaaa
    24001 ttcagtgatt tcagcccgga aaagccaccg gatgtcggta cggccaattt cacttcagac
    24061 ggattgcttt tcgacatgat gttcgctcct caatgcggct tccatcacct gctcctgagt
    24121 caggttatga tttatcaggt tggcttttag tttcccttca tgcatcacca gtacccgatc
    24181 gctaaggccg agcacttcag gtaattcgga agagatgaca ataacggcaa taccctgctg
    24241 gacgagttgg ttaattaatt tgtagatctc gtatttcgcg ccaatatcga tacccctggt
    24301 aggttcatca agaatgagga tgcgcgggtt aagtaacaga cagcgagcga ggatcgcttt
    24361 ttgctgattg ccgccgctca aacgtccaat agcaaggtcg ggggacgacg ttttaacttt
    24421 gagttgctga attgattcca gaatacattt ttgctctgcc gcgtcatcaa gctggctaat
    24481 gccaccggta aatttattga gtgcggcgag ggtaatattt ttaccaaccg ccattaccgg
    24541 aacgatgccg tcgcgctttc tgtcttcggg taccatcgca atcccctggg cgatggcttg
    24601 ctgacagtta cgaatatcta cctgtttgcc atcaatataa atttttcctt cccattgtcc
    24661 gggccacacg ccaaacaggc actgaatggt ctcggtacgt ccggcaccaa cgagtccggc
    24721 aatacccagt atttcgccac gtttcaggga aaacgagaca tcattaactc gtttaatatg
    24781 acgattgacc ggatgccatg ccgtcagatg ttcaatacgt aatatttcat ctccggtggt
    24841 atgtggttca ttagggtaaa gcgcggttaa ctctcgcccg accatcatgg tgataatatc
    24901 gtcttcactc attccggcag catcacgcgt accaatgtgc tgtccgtcgc gaataacgca
    24961 aatcgtatcg gaaatcgctt tgacttcgtt gagtttgtgc gaaatataaa tacaggcgat
    25021 accgtgctgt tgtagatcgc gaataatatc cagtaaaacc gacgtttcct gctcagttaa
    25081 tgaggctgtc ggttcatcga gaattaacaa gcgcacctgt ttattaagtg ccttggcaat
    25141 ttcaaccagt tgttgttgcc caagccctaa atcgccaacg cgggtatcag gtgaaatgga
    25201 taaactgacc tgtgcgagca gcttctgaca gcgtagcgtc atcaggtcat aatccataat
    25261 gccattgtgg gttatttcgt tacccaggaa gatattttcc agcacggtca attctttcac
    25321 cagggccaat tcctggtgaa tgatggcgat acctttgcgt tcggtatcgc ggatgtgact
    25381 cgcctgaatc tcttctcccg caaaaataat ttcgccttcg taggagccat ggggataaat
    25441 accacacagc actttcatca gtgttgattt accagaccca ttttccccac aaagtgagac
    25501 gatttcgcca gcattcaacc gcaagcagac gttatcaatc gccttcacac tgccgaaggt
    25561 tttggtaata ttcttcattt caagtagata aggcataacg actccaccta agccaattca
    25621 ttcacgcggc atggagagaa atcacgcccc cgctccgcgc cggggcgtaa cgcttacagc
    25681 tcgctctctt tgtggaatcc gtctttaact accgtgtctt tgatgttgtt tttattcaca
    25741 tcgatcggtg tcaggaggcg ggagggcaca tctttcaggc cattattcag tgtggtatct
    25801 gcttttggtt cctgaccatt gcccaactca acggcaattt ctgcggcagt atttgccaac
    25861 aacgtaatag gtttatacac cgtcatagtt tgcgtaccgg cagcaatacg tttaatacct
    25921 gcgagatccg catcctggcc ggaaattgct actttccctg ataaaccttg cgcgcttaat
    25981 gcctgaattg ccccacctgc ggtggcatcg tttgaggcaa ctacagcatc aattttgtta
    26041 ttattggcgg ttagcgcgtt ttccataatt ttcaatgcgt tttccggtaa ccagccatca
    26101 acccattggt caccaacgac tttaattttt ccggaatcaa cgtaaggttt taacactttc
    26161 atttgtccgg cgcggaacag cttggcgttg ttatctaccg gcgagccgcc catcaggaag
    26221 taattacctt gcggaacaat atcgaccagg gcttttgcct gcagttcacc gactttttcg
    26281 ttatcgaaag aaatataaaa atcgatatcc gcatcgttaa tcatacgatc gtaagctaat
    26341 actttaatac cttcttgttt ggcttctttt acaacgttac ttaatacctg accgttatac
    26401 ggaataatga caagaacatc gacaccccgg tttatcatgt tttcaatctg cgacatttgt
    26461 gtttcttcat tgccatttgc agactgtaca aatactttcg cgccgagaga ttctgccttt
    26521 ttcacaaaaa tatctcgatc tttttgccag cgttcaagac ggagatcatc aatcgccata
    26581 cctattttga cttctttggc gtgtgccgca acgttggtaa gcagaagtga ggtgcaaagg
    26641 gtgagtagaa tgttctttat tttcatggtg tagggccttc tgtagttaga ggacagcttt
    26701 aataagtaac aatcaccgcg ataaacgtaa ccaattttta gcaactaaac aggggaaaac
    26761 aattacagat ttttatcttt cgattacgat ttttggttta tttcttgatt tatgaccgag
    26821 atcttacttt tgttgcgcaa ttgtacttat tgcatttttc tcttcgagga attacccagt
    26881 ttcatcattc cattttattt tgcgagcgag cgcacacttg tgaattatct caatagcagt
    26941 gtgaaataac ataattgagc aactgaaagg gagtgcccaa tattacgaca tcatccatca
    27001 cccgcggcat tacctgatta tggagttcaa tatgcaagcc tattttgacc agctcgatcg
    27061 cgttcgttat gaaggctcaa aatcctcaaa cccgttagca ttccgtcact acaatcccga
    27121 cgaactggtg ttgggcaagc gtatggaaga gcacttgcgt tttgccgcct gctactggca
    27181 cacattctgc tggaacgggg cggacatgtt tggtgtgggg gcgtttaatc gtccgtggca
    27241 gcagcctggt gaggcactgg cgttggcgaa gcgtaaagca gatgtcgcat ttgagttttt
    27301 ccacaagtta catgtgccat tttattgctt ccacgatgtg gatgtttccc ctgagggcgc
    27361 gtcgttaaaa gagtacatca ataattttgc gcaaatggtt gatgtcctgg caggcaagca
    27421 agaagagagc ggcgtgaagc tgctgtgggg aaccgctaac tgctttacaa accctcgcta
    27481 tggcgcgggt gcggcgacga acccagatcc tgaagtcttc agttgggcgg caacgcaagt
    27541 tgttacagcg atggaagcaa cccataaatt gggcggtgaa aactatgtcc tgtggggcgg
    27601 tcgtgaaggt tacgaaacgc tgttaaatac cgacttgcgt caggagcgtg aacagctggg
    27661 ccgctttatg cagatggtgg ttgagcataa acataaaatc ggtttccagg gcacgttgct
    27721 tatcgaaccg aaaccgcaag aaccgaccaa acatcaatat gattacgatg ccgcgacggt
    27781 ctatggcttc ctgaaacagt ttggtctgga aaaagagatt aaactgaaca ttgaagctaa
    27841 ccacgcgacg ctggcaggtc actctttcca tcatgaaatt gccaccgcca ttgcgcttgg
    27901 cctgttcggt tctgtcgacg ccaaccgtgg cgatgcgcaa ctgggctggg acaccgacca
    27961 gttcccgaac agtgtggaag agaatgcgct ggtgatgtat gaaattctca aagcaggcgg
    28021 tttcaccacc ggtggtctga acttcgatgc caaagtacgt cgtcaaagta ctgataaata
    28081 tgatctgttt tacggtcata tcggcgcgat ggatacgatg gcactggcgc tgaaaattgc
    28141 agcgcgcatg attgaagatg gcgagctgga taaacgcatc gcgcagcgtt attccggctg
    28201 gaatagcgaa ttgggccagc aaatcctgaa aggccaaatg tcactggcag atttagccaa
    28261 atatgctcag gaacataatt tgtctccggt gcatcagagt ggtcgccagg agcaactgga
    28321 aaatctggta aatcattatc tgttcgacaa ataacggcta actgtgcagt ccgttggccc
    28381 ggttatcggt agcgataccg ggcatttttt taaggaacga tcgatatgta tatcgggata
    28441 gatcttggca cctcgggcgt aaaagttatt ttgctcaacg agcagggtga ggtggttgct
    28501 tcgcaaacgg aaaagctgac cgtttcgcgc ccgcatccac tctggtcgga acaagacccg
    28561 gaacagtggt ggcaggcaac tgatcgcgca atgaaagctc tgggcgatca gcattctctg
    28621 caggacgtta aagcattggg tattgccggc cagatgcatg gagcaacctt actggatgct
    28681 caacaacggg tattgcgccc tgccattttg tggaacgacg ggcgctgtgc gcaagagtgc
    28741 actttgctgg aagcgagagt tccgcaatca cgagtgatta ccggcaacct gatgatgccc
    28801 ggatttactg cgcctaaatt gctatgggtt cagcggcatg agccggagat attccgtcaa
    28861 atcgacaaag tattattacc gaaagattac ttgcgtctgc gtatgacggg ggagtttgcc
    28921 agcgatatgt ctgacgcagc tggcaccatg tggctggatg tcgcaaagcg tgactggagt
    28981 gacgtcatgc tgcaggcttg cgacttatct cgtgaccaga tgcccgcatt atatgaaggc
    29041 agcgaaatta ctggtgcttt gttacctgaa gttgcgaaag cgtggggtat ggcgacggtg
    29101 ccagttgtcg caggcggtgg cgacaatgca gctggtgcag ttggtgtggg aatggttgat
    29161 gctaatcagg caatgttatc gctggggacg tcgggggtct attttgctgt cagcgaaggg
    29221 ttcttaagca agccagaaag cgctgtacac agcttttgcc atgcgctacc gcagcgttgg
    29281 catttaatgt ctgtaatgct gagtgcagca tcgtgtctgg attgggtcgc gaaattaacc
    29341 ggcctgtgca atgtcccagc tttaatcgct gcagctcaac aggctgatga aagtgccgag
    29401 ccagtttggt ttctgcctta tctttccggc gagcgtacac cacacaataa tccccaggcg
    29461 aagggggttt tctttggttt gactcatcaa catggcccca atgaactggc gcgagcagtg
    29521 ctggaaggcg tgggttatgc gctggcagat ggcatggatg tcgtgcatgc ctgcggcatt
    29581 aaaccgcaaa gtgttacgtt gattggtggc ggggcgcgta gtgagtactg gcgtcagatg
    29641 ctggcggata tcagcggtca gcagctcgat taccgtacgg ggggggatgt ggggccagca
    29701 ctgggcgcag caaggctggc gcagattgcg gcgaatccag agaaatcgct cattgagttg
    29761 ttgccgcagc taccgttaga acagtcgcat ctaccagatg cgcagcgtta tgccgcttat
    29821 cagccacgac gtgaaacgtt ccgtcgcctc tatcagcaac ttctgccatt aatggcgtaa
    29881 acgttatccc ctgcctgacc gggtggggga taattcacat ctatatatct cactaattaa
    29941 ttaatattta gtatgaattt attctgaaaa tcatttgtta atggcatttt tcagttttgt
    30001 ctttcgttgg ttactcgtaa tgtatcgctg gtagatatgg agatcgttat gaaaacctca
    30061 aaaactgtgg caaaactatt atttgttgtc ggggcgctgg tttatctggt tgggctatgg
    30121 atctcatgcc cattgctaag tggaaaaggc tattttcttg gcgtgttaat gacagcaact
    30181 tttggtaact atgcatatct tcgcgcagaa aaactcgggc aactggataa tttttttacc
    30241 catatctgcc agttagttgc gttaatcact atcggtctct tgtttatcgg tgttttaaat
    30301 gcacctatca atgcttatga aatggtgatc taccccatcg ccttttttgt ctgcttgttt
    30361 ggtcaaatgc gtttgtttcg ctcggtatga gcaacataaa gctcttacac attcaggaat
    30421 gaaaggaata ctgtgatgga caacaaaata tcaacctatt caccggcctt tagtattgtg
    30481 tcatggatag ctctcgttgg tggtatcgtt acctatctgt tagggctatg gaatgcagag
    30541 atgcagttaa atgaaaaagg gtattatttt gccgtactgg tgttaggact gttttctgct
    30601 gcgtcttatc aaaagaccgt tcgggacaag tatgaaggca taccgaccac ttccatttat
    30661 tatatgacct gcctgactgt ctttattatc tctgttgcat tactgatggt aggtctgtgg
    30721 aatgcgacat tattactcag tgaaaaaggt ttttatggac tggctttctt cttaagcttg
    30781 tttggtgcag tagcggtgca gaagaatatt cgtgataccg gaataaaccc gccaaaagaa
    30841 acacaggtta cccaggaaga atacagcgaa taattcacgt aagcccggtc agtccaatgt
    30901 gaacgggctt ttacttaact cactaatctg tttctgtcga ttcgttgtac cagcatagaa
    30961 agtaacaaac tcgctgccaa cgtcgcgcaa aagatccaaa taatatccag tattggccaa
    31021 tttttaagct caattccccg ggtgcgcagc gcatggataa tcaaggcgtg gaatccgtat
    31081 atacccaatg aatggcggga gattaagcca agtccgcgaa tggtacgcgt atccagcgtg
    31141 tttttaacca gagtcaatag cgcgattgcg cagataaaaa ccatcggccc acagtaaaga
    31201 taccaggtat cggcaaaatt tccgcgccac tgcaattcat ataatgtccc gcgagagata
    31261 ataaaaaccc ccgtcgcaaa cagcgcggcg ctcacccacg acagtgcttt atgctgtgtg
    31321 tccatcatcc ctatagcgcg gcccaacatg ccatacagaa tgtagtaaaa agtatcgcca
    31381 ttgatatata agttaattgg cagccattca aaaccgtcaa ttttctgcgg cactgtgttt
    31441 gggttagcga taatgccaat caccaccatt agcaccagca acatttttcc gccgacgttc
    31501 ttcacctgaa tcagcggtga aaccagataa atcaccgcaa tcgcgaagaa aaaccacaag
    31561 tggtaaaaca ctggcttttg cagcaggttt ttcagcgcta actccatatt gatggaggta
    31621 aacagcgcaa tgtagagcag tgcgattgcg ctataaaaaa tcagacataa gccgatacgc
    31681 aagaaatggc gcggctgggc gctgcgttcg ccaaaaaaga gatagccgga aatcatgaaa
    31741 aatagcggca cgctgacacg agaggcagaa ttcagaacat tggcgatatc ccaggtgacg
    31801 gggctaacac tatgagcatt ggtcacatac caggtagtgg tgtgaatcat caccaccatt
    31861 aaacacgcta tccctcgcag gttatcaatc cagtaaattt tgggctgcat ctgtgtctct
    31921 gtatctggtt aaaaaaagtc tgaccgataa atcattcagt tggcgcactg gaataatctg
    31981 agttttatca ctacagctta tagaggctta aggaaattcg taagatatca gccactatac
    32041 cgatataaat aataagactc acctgcaaac cagacggtaa tttaatgatg atgaacgctt
    32101 tctttccggc aatggcgctt atggtgctag tgggttgttc tacaccgtca cccgtgcaga
    32161 aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa tatggaagcg ttatgcaagg
    32221 atcaggcggc aaaacgttat aacaccggcg agcaaaaaat cgacgtcacc gccttcgaac
    32281 agttccaggg aagctatgaa atgcgcggtt ataccttccg taaagagcag tttgtctgtt
    32341 cttttgacgc agatggccat tttttgcatc tttccatgcg ttaagccctg ctttttcccg
    32401 tttcgtactg tatatcttcc atccagcggg tatactgatc ccttccttta aatccacacg
    32461 tatccagcac gaaataatat gcaaaagttt gataccagga ccttccaggg cttgatcctg
    32521 accttacagg attactgggc tcgccagggc tgcaccattg ttcaaccatt ggacatggaa
    32581 gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg cgctggggcc agaaccgatg
    32641 gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg gtcgctacgg cgaaaacccc
    32701 aaccgtttac agcactacta tcagttccag gtggtcatta agccatcgcc ggacaatatt
    32761 caggagctgt acctcggttc tctgaaagag ctgggcatgg acccgactat tcacgacatc
    32821 cgtttcgtgg aagataactg ggaaaacccg actctgggtg cctggggact gggctgggaa
    32881 gtgtggctaa acggcatgga agtgacgcag ttcacttact tccagcaggt tggtggtctg
    32941 gagtgtaaac cggttaccgg cgagatcacc tacggtctgg aacgtctggc catgtacatt
    33001 cagggcgtag acagcgttta cgacctggtc tggagcgacg gcccgctggg taaaaccacc
    33061 tacggcgacg tgttccatca gaacgaagtg gagcagtcca cttacaactt cgaatacgcg
    33121 gatgtggact tcctgttcac ctgctttgag cagtacgaga aagaagcgca gcagctgctg
    33181 gcgctggaaa atccgctgcc gctgccagcc tacgagcgta ttctgaaagc cgcccacagc
    33241 ttcaacctgc tggatgcgcg taaagccatc tccgtcaccg agcgtcagcg ctacattctg
    33301 cgcattcgca ccctgaccaa agcagtggca gaagcatact acgcttcccg tgaagccctc
    33361 ggcttcccga tgtgcaacaa agataagtaa gaggcggcta tgtctgagaa aacttttctg
    33421 gtggaaatcg gcactgaaga gctgccacca aaagcactgc gcagcctggc tgagtccttt
    33481 gctgcgaatt ttactgcgga gctggataac gctggcctcg cacacggcac cgttcaatgg
    33541 tttgctgctc cgcgtcgtct ggcgctgaaa gtagctaacc tggcggaagc gcaaccggat
    33601 cgtgaaatcg aaaaacgcgg cccggcgatt gcccaggcgt tcgacgctga aggcaaaccg
    33661 agcaaagcgg cagaaggttg ggcgcgtggt tgcggtatta ccgttgacca ggctgagcgt
    33721 ctgactaccg ataaaggcga atggctgctg tatcgcgccc atgtgaaggg cgaaagcacc
    33781 gaagcactgc tgccgaatat ggttgcgact tctctggcga aactgccgat cccgaaactg
    33841 atgcgttggg gcgcaagcga cgtgcacttc gtgcgtccgg tgcacaccgt gaccctgctg
    33901 ctgggcgaca aagtcattcc ggcaaccatt ctgggcattc agtccgatcg cgtgattcgc
    33961 ggccaccgct ttatgggcga accggaattc accatcgaca acgccgatca gtatccggaa
    34021 attctgcgtg agcgcgggaa agtcatcgcc gattacgaag aacgtaaagc gaagattaaa
    34081 gccgatgccg aagaagcagc gcgtaagatt ggcggtaacg ctgacttaag cgaaagcctg
    34141 ctggaagaag tggcttcgct ggtggagtgg ccggtcgttc tgaccgcaaa attcgaagag
    34201 aaattcctcg cggtgccggc tgaagccctg gtttacacca tgaaaggtga ccagaaatac
    34261 ttcccggtgt atgcgaacga tggcaaactg ctgccgaact tcatcttcgt tgccaacatc
    34321 gaatcgaaag atccgcagca aattatctct ggtaacgaga aagtcgttcg tccacgtctg
    34381 gcggatgccg agttcttctt caataccgac cgtaaaaaac gtctggaaga taacctgccg
    34441 cgcctgcaaa ccgtgctgtt ccagcaacag ctgggtacgc tgcgcgacaa aactgaccgc
    34501 atccaggcgc tggctggctg gattgctgaa cagattggcg ctgacgttaa ccacgcaacc
    34561 cgtgcgggcc tgctgtctaa gtgtgacctg atgaccaaca tggtcttcga gttcaccgac
    34621 acccagggcg ttatggggat gcactatgcg cgtcacgatg gcgaagcgga agatgtcgcg
    34681 gtggcgctga atgagcagta tcagccgcgc tttgccggtg atgacctgcc gtctaacccg
    34741 gtagcctgtg cgctggcgat tgctgacaag atggatactc tggcgggtat cttcggtatc
    34801 ggccagcatc cgaaaggcga caaagacccg tttgcgctgc gtcgtgccgc acttggcgtg
    34861 ctgcgaatta tcgttgagaa gaacctcaac cttgatctgc aaacgctgac cgaagaagca
    34921 gtgcgtctgt atggcgataa gctgactaac gccaacgtgg ttgatgatgt tatcgacttt
    34981 atgctcggtc gcttccgcgc ctggtatcag gacgaaggtt acaccgttga caccatccag
    35041 gcggtactgg cgcgtcgtcc gactcgtccg gctgatttcg atgcccgcat gaaagcggta
    35101 tcgcacttcc gtacccttga agcagctgct gcactggcgg cggcgaacaa gcgtgtctcc
    35161 aacattttgg cgaaatctga cgaagtgctg agcgaccgcg tgaatgcctc taccctgaaa
    35221 gagccggaag aaattaaact ggcgatgcag gttgtggtgc tacgtgacaa gctggagccg
    35281 tactttgcgg aaggtcgtta ccaggatgcg ctggtcgaac tggcagagct gcgtgagccg
    35341 gttgatgctt tcttcgataa agtgatggtc atggttgatg acaaagaatt gcgtctcaac
    35401 cgtctgacca tgctggagaa actgcgcgaa ctgttcctgc gcgttgcgga tatttcgctg
    35461 ttgcagtaat aacgccgtta ttaaaaagcc tgccatctgg caggcttttt ttattatcgc
    35521 taaataatac agcaaccttt aataatcttc tgctgaataa agattatctc atatattaat
    35581 tttatgaggt ttttttagga ttatatcaag gagaagaaac aaacttatta agctagaata
    35641 gccacgggtg cttgagactg tttgtctcag gtattcaccg aaaggcagac agagaaaagc
    35701 cccacctgac tataaatcaa agtgaggcta ccctatgcct gaacaccata aggttagcct
    35761 cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc
    35821 tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg
    35881 tgaattgcat attaaacagg ggagttatga gctggcggca tttttagcct gcaatttaaa
    35941 agagtaagag tcttcggcgg gaagttattc ccgcctttct tacggcgttg cgcattctca
    36001 ttgcacccaa atttattttt cacaaaaata atactagatt ttattacgcg atcgattatt
    36061 tatttcctga aaaacaaata aaaaaatccc cgccaaatgg cagggatctt agattctgtg
    36121 cttttaagca gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt
    36181 tcgatggtga aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata
    36241 gcagagaagt gtacgaacac gtctttagag ccatcgtcag gagtgatgaa gccgaagcct
    36301 ttgtcagcgt tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt
    36361 taataattaa gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag
    36421 gaatgtgcac tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg
    36481 ctttaatggt ctgtacgtca aaccgttgat gtacattaag ccacgcattg gcgggtgatg
    36541 caacaattat ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc
    36601 gtaaacagca ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt
    36661 ggcttgaatc aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc
    36721 ccattccttt accatggcga ctgatacgcc caaaacccgg gcgaaatcat cgatttttaa
    36781 ccctgtccct tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt
    36841 aattttctgc gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat
    36901 tggatcttta tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc
    36961 gaagctcatt aagaatagtc gggaaaagca gacacagcgg ggtgaagtca gggcatgctc
    37021 tggctatgat tcgattcagc ctgtcattta gcgtgactta tagccaccta acgatctgat
    37081 aaatgccgat atatctctta ccactcctgt tttgtaaaga tttcgaaaat ctaaccttct
    37141 gcgaaggcgt aaatctctgg ctacaaaaaa ggcaacggca ctatcatttt ttaacaatga
    37201 aacaacaaat taaccttgtt tcattttctc catgatttta ccaggaagat tacgtagaaa
    37261 gggtatcctg caagcctgtc ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg
    37321 tgcgcttttt ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg
    37381 gtttgcgcct cttgcggcgt tattaatggt agttagcctg agtgggtgtt ttgataaaga
    37441 aggcgatcag cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc gtagcggtga
    37501 acgtctacca accctgactg ccgatcagaa aaaacagttt ggtccttttg tctctgatta
    37561 cgcgattctg tatggttatt ctcagcaggt gaatcaggcg atggattccg gtctgcgccc
    37621 ggtcgtagac agcgttaacg caattcgcgt accgcaggat tacgttacgc aaagcggtcc
    37681 gctgcgtgaa atgaacggtt cgctgggtgt gctggcgcaa caactgcaaa atgcgaagtt
    37741 gcaggccgat gctgcgcact ctgcgctaaa acagaccgat gatttgaaac cggtctttga
    37801 tcaggcattc accaaagtgg taacgacgcc agctgatgca ttgcaaccgt tgattccggc
    37861 tgcacaaact ttcacgcaac aactggtgat ggttggagac tacattgctc agcaaggtac
    37921 tcaggtgagc tttgtcgcaa atggcatcca gttcccgact tcacagcagg caagtgaata
    37981 taacaaactg attgcgccat taccggcaca acatcaggcg tttaatcagg cctggactac
    38041 agcagttact gcaacccaat aaagagtaaa agcccgagcg attatcgggc tttacttcac
    38101 gcagtcgcgg cttagtccgc gacgtgcgga ttcacacagt tcttctcaac ctttccttgt
    38161 aacgcatcaa tcaaattatc cacggcacag gcggccatgc cataacgcgt ctcatgggtg
    38221 gcagatccaa tatgcggtac tgcgacgacg ttggccattg agagcaacgg cgaatctacg
    38281 gacagtggct cttgttcgaa gacatccagc ccggcggcgt gaatttcgcc tttctgcaat
    38341 gcagcaatca gtgcattttc atcaaccacc gggccacgtc cggcattaat gaaaatggcg
    38401 gaggatttca ttttggcgaa ttgttctgcg ccaaacagat gatgcgtctc atcagttaac
    38461 ggcaggatca ggcaaacgaa atctgactct tgtaacagag tatccaaatc gcagtagcgg
    38521 gcgttgaagc gttcttctgc ttctttatgg tggcggcgcg cgttatagag gatgggcatg
    38581 ttgaagccaa agtgcgcacg ttgtgccagc gccataccga tccgtcccat cccgacaatg
    38641 cccagtgttt tatggtgaac gtcagtgccg taccagtccg ggcctatgct cgcggtccat
    38701 tcgcctgctt ttacccgttc tgctacctcc acaacccgac gagcggtaga caacaccagc
    38761 gccatcagcg tatcggcgac ggtttctgtt aatacggttg gcgtgtgcat cagcagaatt
    38821 tttcgggcgg taagcgcatc gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg
    38881 gcacgcagtt tcggcatttt ttccagcaat gcggcatcaa cattctcgtt tgaacccagt
    38941 aaaccttcag cttcggcaaa aattgctgca ttttgttcga cggtttgtgg gctgaggttt
    39001 gccacctggt gaacggtgaa atgctcttgc aggcgttgca gtaaatcatc aggtaaggct
    39061 ttgtagagga taacggacgg cttcatgctt ctctccattc actgataagg cgatcacctt
    39121 agcctgaaat atgatgaagg aaaactgaag aatgccaggt gatttctgca tcacctggca
    39181 tgaaagggat tacagcgggc ttaaggtaat ttctacacgg cggttttgcg ccttaccttc
    39241 tgcggtgctg ttgctggcga ttgggttagc cgggccaagg ccctgagtac ggatgcggct
    39301 ggcgtccacg ccctgggtga tcaacgcgct ggcaacggaa tccgcacgtt gctgggagag
    39361 acgcatgttc aggtcgtgac cacccgtgct gtcggtataa ccaatcacgt taaccgccgt
    39421 tttcggatac tctttcagca ccattgccac gccggtcagg gtgttagcgc ccgcaggttt
    39481 cagggtcgcg ctgctgctgt cgaaggtcac attgttcggc atattgagga taatgttatc
    39541 cccgctgcgg gttacgctaa caccagtgcc gcgcattttg tcacgcagct tcgcttcctg
    39601 cacatccatg taataaccca cgccgccgcc cagagctgcg cctgctgctg cgccaatcag
    39661 cgcgccttta ccgcgatctt tcttcgaaga agagagcgca ccaatacccg cgcccacgag
    39721 agagcccaga cctgcgccga tagcagattt acctgcttcg cgttcgccgg tgtaagggtt
    39781 agttgtgcag ccagataccg ccagagcgcc actcactacg gcggcaataa gataaacacg
    39841 tttcttcatt gttaatcctt aataaccttt ttattctttg ccacgggttc cgtggcggga
    39901 gattatgccg cgtgaacatg aagattattc ctgggaatac tcggaaattt gtaagtaata
    39961 tttaactgct caatacatct aacctttcag gagttttcgg tttggccaac tcatcctcac
    40021 gatattccgt tctgactgcc gcccattggg ggcccatgct ggttgaaacc gacggcgaaa
    40081 ccgtgtttag ctcgcatggc gcgttagcca caggaatgga aaactccttg cagagcgcgg
    40141 ttcgcgacca ggttcacagc aatacacggg tacgatttcc aatggtgcgc aaaggctttc
    40201 ttgcgtcacc ggaaaatccg caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt
    40261 gggatgaggc gctggatctt attcaccaac aacataaacg cattcgtgag gcttatggtc
    40321 cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg cataaggcct
    40381 cgacattatt acaacgctat atggcgctgg caggcggtta taccgggcat ctgggggatt
    40441 attcgaccgg cgcggcacag gcgatcatgc cgtatgtcgt gggtggtagc gaagtttatc
    40501 aacagcaaac cagttggccg ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg
    40561 ctaacccact caatacgctg aaaattgcgt ggaatgcatc tgatgagcag gggctttctt
    40621 acttttccgc actgcgtgac agcgggaaaa agctgatctg tattgatcca atgcgatcgg
    40681 aaaccgtcga tttctttggc gataaaatgg agtgggtggc accgcacatg ggcaccgatg
    40741 ttgcgctgat gctggggatc gcccatacgc tggtggaaaa tggttggcac gacgaagcgt
    40801 ttctgacgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg
    40861 acggaatagc gaaaaacgcc gaatgggcgg cagagatttg tggggttaac gcagcgaaaa
    40921 tccgcgagct ggcggctctt ttccaccaaa acacgaccat gctgatggct ggctggggaa
    40981 tgcagcgcca acagtttggt gagcaaaaac actggatgat cgtcacgctg gcggcaatgt
    41041 tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttatcatttt gccaatggtg
    41101 gtaaccccac gcggcgttct gcggtgctct cttccatgca gggcagcttg ccgggtggct
    41161 gcgatgcggt ggataaaatc cctgttgccc gcattgttga agcactggaa aaccctggtg
    41221 gcgcatatca acacaacggt atgaaccgac atttcccgga tattcgtttt atctggtggg
    41281 cgggcggtgc caactttact catcatcagg ataccaatcg tctgatccgt gcctggcaaa
    41341 aaccggagct ggtggtgatc tctgaatgct tctggacggc ggcggcgaaa cacgcggata
    41401 tcgttctgcc tgcgactacc tcttttgagc gtaatgatct caccatgacc ggtgattaca
    41461 gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg
    41521 attttgatgt ttttgccgag ttgagtgaac gttgggagaa gggcggctat gcgcggttta
    41581 cggaaggaaa aagtcagctg caatggctgg aaacgtttta taacgttgcc cggcagcgcg
    41641 gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa
    41701 ttgagatgcc ggaaaacccg gacagcgagc ggtttattcg cttcgctgat ttttgccgcg
    41761 atccgctggc gcatccgtta aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta
    41821 ttgccgatta cggttacccg gattgccctg ggcatccaat gtggctggag ccggacgaat
    41881 ggcagggcaa tgccgaacca gaacagttgc aggtactttc tgcccatccg gcgcaccgcc
    41941 tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtagca aaccgtgagc
    42001 ctgtcaccat tcatcctgac gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc
    42061 gggtgtggaa ctcccgtggg cagatccttg ccggagcggt cattagcgag ggaattaaac
    42121 ctggcgtgat ttgcattcac gaaggggcat ggccggatct ggatttaacc gctgacggta
    42181 tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg cggctgggga
    42241 atggctgtgc gggtaatacg gcgctggcat ggctggaaaa atacaacggt ccggaactga
    42301 cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt gggtttcatc
    42361 ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg
    42421 atttttttga taaacctcca gcatcagatg gggatagcgc tgctgcacat actgcatcag
    42481 cgccttacca ataccgcgtc tgacggcctt cggtgcgaca aacattgctg ccagaaatcg
    42541 gccttccata atgctgacaa aaccgagaag cttaccgtcc tcttcccaga cccagttttg
    42601 cgcgttggca agataggcat cccgcaccag cggaatgcag tcacgccagt aattcgcttt
    42661 tataaaggga tgcccccagg ttgtactttc cagccacagt tcgaggatcg cggggagttc
    42721 tgaacgttgc gcttcccgaa tcatggttta tttcccggat agcaacagca gccaaccaca
    42781 tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt gccgacaaac
    42841 ttaaaaccac gttttttcag tgccttagat agggcgtcgg aggcggacgt agatgtggga
    42901 atttcgctca acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca
    42961 aagtcggcaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc attaccaata
    43021 attgcctgaa ttttccctcg atggcggata atcccggcgt cctgtaccag tctttcgaca
    43081 tcctcttcct gcattgctgc gaccttcacc ggatcgaact gatgaaagca ggcgcgatag
    43141 ttttcgcgtt ttttgaggac ggtgatccac gataacccag cctgctgccc ttcaaggcag
    43201 atcatttcga acagtttttt actgtcagtt tcaggcacgc cccattcatt atcatggtag
    43261 gcaatataaa gcgggtcctg actcacccag ccgcaacgtt ccatactttc cctcgctatg
    43321 tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa gtcaatattt
    43381 aatacagggc tactatgccc gatatccttc atgcaatgac aataatattg ccgaattcgg
    43441 ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc
    43501 ctgctggcga gcgtggtaat cagtgccttt tttctcaaca cagcttacgc ctggcaacaa
    43561 gaatatatcg ttgatacgca acccggacat tccacagagc gttacacctg ggatagtgat
    43621 catcaacctg attacaacga tattttgtcg caacgtattc aaagtagcca aagggcgctg
    43681 ggactggaag tcaatctggc ggaagaaact cctgtggatg tgaccagcag tatgagtatg
    43741 ggctggaatt ttcctttata tgaacaggtt acaaccggcc cggtcgcggc attacattac
    43801 gatggcacaa ccacctcgat gtataacgag tttggcgaca gtactaccac gctgaccgat
    43861 ccgttatggc atgccagcgt gagtagctta ggctggcgtg ttgactcccg gcttggcgat
    43921 ctccgaccct gggcgcaaat cagctataac cagcaatttg gcgagaatat ctggaaggcg
    43981 caatcaggcc tgagccggat gacggcgaca aaccagaacg gcaactggct ggatgtcacc
    44041 gtaggcgctg atatgttgct caatcaaaat attgccgcct atgccgcgct aactcaggca
    44101 gaaaatacca ctaataatag cgactatctg tatacgatgg gggttagcgc cagattttaa
    44161 cgtaacagtc acaattgaaa ccattgaata acaacagttg tggcgatagt gggtgctaac
    44221 ttaccaaata ataaatttgg tgaataattg tcgcgtcatt cattcctgaa ctaaggcatt
    44281 tcattccgtt ctgatggcat ttcatgccgt ttttccccag gcataaaatg cacttcgtta
    44341 tggttgtcgg cagagatttt tcctttttat tactgcagga atactgccat gacaccttca
    44401 aattatcagc gtacccgctg gctgacactc atcggtacta tcattaccca gtttgcactg
    44461 gggtcggttt atacctggag cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg
    44521 gtaagccagg tcgctttctc tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct
    44581 gttgcgggca aattgcagga acgttttggc gttaaacgcg tcaccatggc ttccggcatt
    44641 ttgctgggat taggcttctt cctgaccgcg cattccaaca acctgatgat gctgtggtta
    44701 agcgccggtg tgctggtcgg tctagcggat ggcgcgggtt acctgctgac gctctctaac
    44761 tgcgtgaagt ggttcccgga gcgtaaaggt ctgatatccg cgttcgctat cggttcttat
    44821 ggtctgggta gcctgggttt caaatttatc gacacgcagc tgctggaaac ggtgggtctg
    44881 gaaaaaacct ttgtgatttg gggagcgatt gcgctggtga tgattgtttt cggcgcaacg
    44941 ttaatgaaag acgcaccaaa acaggaagtg aaaaccagca atggcgtagt ggagaaagat
    45001 tacacgctgg cagagtcgat gcgtaaaccg cagtactgga tgttagcggt aatgttcctg
    45061 actgcctgca tgagcggcct gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg
    45121 gcacaccttg atgtggtttc cgcagccaat gcagtaacgg ttatttccat cgctaacctt
    45181 tcaggtcgtc tggtgctggg cattctgtct gacaaaattg cccgtatccg tgttatcacc
    45241 attggtcagg tgatttcgct ggtgggtatg gcggccctgc tgtttgcacc attaaatgca
    45301 gtgacgttct ttgcagcgat tgcctgcgtg gcatttaact ttggcggcac tattaccgtc
    45361 tttccatcac tggtcagtga gttctttggc ctcaataacc tggcgaaaaa ctacggtgtg
    45421 atttacctcg gtttcggtat cggtagcatt tgtggttcga ttatcgcctc actgtttggc
    45481 ggcttctatg tgaccttcta cgtgattttc gccctgctga ttctgtcatt ggcgctttct
    45541 acgacaattc gtcagccaga gcagaaagta ttgcgtgagg cgcatattta gaaatatgtt
    45601 gctactgaaa taaatgcgag gagggagact tcaaagtctc ccttttttta tggacgcata
    45661 tta
//
LOCUS       DAZFYC010000023        44850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFYC010000023 DAZFYC010000000
VERSION     DAZFYC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.023"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(339..881)
                     /gene="ycfP"
                     /locus_tag="ACHKW5_001485"
     CDS             complement(339..881)
                     /gene="ycfP"
                     /locus_tag="ACHKW5_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO7510903.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(904..1929)
                     /gene="nagZ"
                     /locus_tag="ACHKW5_001486"
     CDS             complement(904..1929)
                     /gene="nagZ"
                     /locus_tag="ACHKW5_001486"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO7510904.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFKRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1940..2764)
                     /gene="thiK"
                     /locus_tag="ACHKW5_001487"
     CDS             complement(1940..2764)
                     /gene="thiK"
                     /locus_tag="ACHKW5_001487"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO7510905.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFHSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2745..3386)
                     /gene="lpoB"
                     /locus_tag="ACHKW5_001488"
     CDS             complement(2745..3386)
                     /gene="lpoB"
                     /locus_tag="ACHKW5_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO7510906.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3400..3777)
                     /locus_tag="ACHKW5_001489"
     CDS             complement(3400..3777)
                     /locus_tag="ACHKW5_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO7510907.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3780..4139)
                     /gene="hinT"
                     /locus_tag="ACHKW5_001490"
     CDS             complement(3780..4139)
                     /gene="hinT"
                     /locus_tag="ACHKW5_001490"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO7510908.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4473..6662
                     /gene="fhuE"
                     /locus_tag="ACHKW5_001491"
     CDS             4473..6662
                     /gene="fhuE"
                     /locus_tag="ACHKW5_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO7510909.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6722..8155)
                     /gene="ptsG"
                     /locus_tag="ACHKW5_001492"
     CDS             complement(6722..8155)
                     /gene="ptsG"
                     /locus_tag="ACHKW5_001492"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO7510910.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8450..9247)
                     /locus_tag="ACHKW5_001493"
     CDS             complement(8450..9247)
                     /locus_tag="ACHKW5_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7510911.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9258..10262)
                     /gene="holB"
                     /locus_tag="ACHKW5_001494"
     CDS             complement(9258..10262)
                     /gene="holB"
                     /locus_tag="ACHKW5_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO7510912.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10259..10900)
                     /gene="tmk"
                     /locus_tag="ACHKW5_001495"
     CDS             complement(10259..10900)
                     /gene="tmk"
                     /locus_tag="ACHKW5_001495"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7510913.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10890..11912)
                     /gene="yceG"
                     /locus_tag="ACHKW5_001496"
     CDS             complement(10890..11912)
                     /gene="yceG"
                     /locus_tag="ACHKW5_001496"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO7510914.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11915..12724)
                     /gene="pabC"
                     /locus_tag="ACHKW5_001497"
     CDS             complement(11915..12724)
                     /gene="pabC"
                     /locus_tag="ACHKW5_001497"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO7510915.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12844..14085)
                     /gene="fabF"
                     /locus_tag="ACHKW5_001498"
     CDS             complement(12844..14085)
                     /gene="fabF"
                     /locus_tag="ACHKW5_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO7510916.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTLPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14173..14409)
                     /gene="acpP"
                     /locus_tag="ACHKW5_001499"
     CDS             complement(14173..14409)
                     /gene="acpP"
                     /locus_tag="ACHKW5_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7510917.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14620..15354)
                     /gene="fabG"
                     /locus_tag="ACHKW5_001500"
     CDS             complement(14620..15354)
                     /gene="fabG"
                     /locus_tag="ACHKW5_001500"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO7510918.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15367..16296)
                     /gene="fabD"
                     /locus_tag="ACHKW5_001501"
     CDS             complement(15367..16296)
                     /gene="fabD"
                     /locus_tag="ACHKW5_001501"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO7510919.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16312..17265)
                     /gene="fabH"
                     /locus_tag="ACHKW5_001502"
     CDS             complement(16312..17265)
                     /gene="fabH"
                     /locus_tag="ACHKW5_001502"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO7510920.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17333..18403)
                     /gene="plsX"
                     /locus_tag="ACHKW5_001503"
     CDS             complement(17333..18403)
                     /gene="plsX"
                     /locus_tag="ACHKW5_001503"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO7510921.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18484..18657)
                     /gene="rpmF"
                     /locus_tag="ACHKW5_001504"
     CDS             complement(18484..18657)
                     /gene="rpmF"
                     /locus_tag="ACHKW5_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO7510922.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18709..19230)
                     /gene="yceD"
                     /locus_tag="ACHKW5_001505"
     CDS             complement(18709..19230)
                     /gene="yceD"
                     /locus_tag="ACHKW5_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO7510923.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19429..20013
                     /gene="yceF"
                     /locus_tag="ACHKW5_001506"
     CDS             19429..20013
                     /gene="yceF"
                     /locus_tag="ACHKW5_001506"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO7510924.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20125..21084)
                     /gene="rluC"
                     /locus_tag="ACHKW5_001507"
     CDS             complement(20125..21084)
                     /gene="rluC"
                     /locus_tag="ACHKW5_001507"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO7510925.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21657..24842
                     /gene="rne"
                     /locus_tag="ACHKW5_001508"
     CDS             21657..24842
                     /gene="rne"
                     /locus_tag="ACHKW5_001508"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO7510926.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVVVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25038..25991)
                     /gene="flgL"
                     /locus_tag="ACHKW5_001509"
     CDS             complement(25038..25991)
                     /gene="flgL"
                     /locus_tag="ACHKW5_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO7510927.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26003..27646)
                     /gene="flgK"
                     /locus_tag="ACHKW5_001510"
     CDS             complement(26003..27646)
                     /gene="flgK"
                     /locus_tag="ACHKW5_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO7510928.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27712..28653)
                     /gene="flgJ"
                     /locus_tag="ACHKW5_001511"
     CDS             complement(27712..28653)
                     /gene="flgJ"
                     /locus_tag="ACHKW5_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO7510929.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKVGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28653..29750)
                     /gene="flgI"
                     /locus_tag="ACHKW5_001512"
     CDS             complement(28653..29750)
                     /gene="flgI"
                     /locus_tag="ACHKW5_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO7510930.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29762..30460)
                     /gene="flgH"
                     /locus_tag="ACHKW5_001513"
     CDS             complement(29762..30460)
                     /gene="flgH"
                     /locus_tag="ACHKW5_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO7510931.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30513..31295)
                     /gene="flgG"
                     /locus_tag="ACHKW5_001514"
     CDS             complement(30513..31295)
                     /gene="flgG"
                     /locus_tag="ACHKW5_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO7510932.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31433..32188)
                     /gene="flgF"
                     /locus_tag="ACHKW5_001515"
     CDS             complement(31433..32188)
                     /gene="flgF"
                     /locus_tag="ACHKW5_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO7510933.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32208..33413)
                     /gene="flgE"
                     /locus_tag="ACHKW5_001516"
     CDS             complement(32208..33413)
                     /gene="flgE"
                     /locus_tag="ACHKW5_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO7510934.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33438..34133)
                     /gene="flgD"
                     /locus_tag="ACHKW5_001517"
     CDS             complement(33438..34133)
                     /gene="flgD"
                     /locus_tag="ACHKW5_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO7510935.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     HGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34145..34549)
                     /gene="flgC"
                     /locus_tag="ACHKW5_001518"
     CDS             complement(34145..34549)
                     /gene="flgC"
                     /locus_tag="ACHKW5_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO7510936.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34553..34969)
                     /gene="flgB"
                     /locus_tag="ACHKW5_001519"
     CDS             complement(34553..34969)
                     /gene="flgB"
                     /locus_tag="ACHKW5_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO7510937.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35124..35783
                     /gene="flgA"
                     /locus_tag="ACHKW5_001520"
     CDS             35124..35783
                     /gene="flgA"
                     /locus_tag="ACHKW5_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO7510938.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35859..36152
                     /gene="flgM"
                     /locus_tag="ACHKW5_001521"
     CDS             35859..36152
                     /gene="flgM"
                     /locus_tag="ACHKW5_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO7510939.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36157..36573
                     /gene="flgN"
                     /locus_tag="ACHKW5_001522"
     CDS             36157..36573
                     /gene="flgN"
                     /locus_tag="ACHKW5_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO7510940.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36613..38148)
                     /gene="murJ"
                     /locus_tag="ACHKW5_001523"
     CDS             complement(36613..38148)
                     /gene="murJ"
                     /locus_tag="ACHKW5_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO7510941.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPALLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38258..39181)
                     /locus_tag="ACHKW5_001524"
     CDS             complement(38258..39181)
                     /locus_tag="ACHKW5_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7510942.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39183..39830)
                     /locus_tag="ACHKW5_001525"
     CDS             complement(39183..39830)
                     /locus_tag="ACHKW5_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO7510943.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSCAARMYEFSDMAEVESTLEQLANREDGPFVVRLARELGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39841..40425)
                     /gene="rimJ"
                     /locus_tag="ACHKW5_001526"
     CDS             complement(39841..40425)
                     /gene="rimJ"
                     /locus_tag="ACHKW5_001526"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7510944.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40661..41869
                     /gene="mdtH"
                     /locus_tag="ACHKW5_001527"
     CDS             40661..41869
                     /gene="mdtH"
                     /locus_tag="ACHKW5_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO7510945.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41933..42580
                     /gene="grxB"
                     /locus_tag="ACHKW5_001528"
     CDS             41933..42580
                     /gene="grxB"
                     /locus_tag="ACHKW5_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO7510946.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42714..43274
                     /locus_tag="ACHKW5_001529"
     CDS             42714..43274
                     /locus_tag="ACHKW5_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7510947.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43380..44426
                     /gene="pyrC"
                     /locus_tag="ACHKW5_001530"
     CDS             43380..44426
                     /gene="pyrC"
                     /locus_tag="ACHKW5_001530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO7510948.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44500..44745
                     /gene="dinI"
                     /locus_tag="ACHKW5_001531"
     CDS             44500..44745
                     /gene="dinI"
                     /locus_tag="ACHKW5_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO7510949.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10856 a        12129 c        11165 g        10700 t
ORIGIN      
        1 taacaacagg ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca
       61 ttggtcatac caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac
      121 cgggtgagat caaaaaacag agtttattgt ttattatttg agcaagcccg gaagggcgat
      181 atagggtgaa gcgtatatcg agggcgtttg gtcgtcctga taagaggcgc atcaggcatt
      241 gatgctcgaa tgtcggatgc ggcgtgaacg ccttatccga cccacagaat ccggagcgca
      301 tcaggtaccg gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct
      361 ttaatgcgct gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag
      421 acaatctcgt aataatgatg cagctcttca gatgtccgct ggctgttaag cgcttcatca
      481 ttacgcgaca aaatcaccag gcaacgatcg cgattcttct cacggaagtt ggtcacacac
      541 ttagtggcaa tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa
      601 gggaacaaat tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc
      661 gcccagtatc cgcccagacc aacgccgcaa atcagcggac gctcgtcgac gttcagttgc
      721 aacattttgt ccacttcttt gagcagatgc tgcatatcat gtttcggatg ccgcgtactg
      781 tagcttatca agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta
      841 cccggactgt tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca
      901 gggttagtga cctgctttct cttcctgcca gcgttcatgt aactgattca gacgggtgct
      961 gatcgctttc cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata
     1021 caaacgtgta acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc
     1081 tttacgatta ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg
     1141 ttcggcataa ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac
     1201 gccgtcaaaa cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc
     1261 cggacgcgga tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt
     1321 ttcgcggatt aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg
     1381 tggatcgcac ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg
     1441 tttcccggtc gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc
     1501 cagggctttt tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc
     1561 gacatccagc acaggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat
     1621 cagccaaccc gcctcttgcg ccagtttgcc gccctcttcc attcccaaca gcgcagcaaa
     1681 ggattgtgct gccggtaagc gggtaaaacc ttcgcgaaaa cgctgcacgc gtccaccttc
     1741 ctgatctacc gccaccacca gatgattgcg cgaagctgcg cggatctggc gcaccagttc
     1801 acgcaactgg gcaggatcat gatagttacg cttaaagaga atcagccctc ccaccagcgg
     1861 atgcgcaagt atttcacgct cttccgcgtc cagttcgtaa ccttcgacat ccaacattac
     1921 tggacccaca ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca
     1981 gcctgataaa ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga
     2041 gcatcagcag ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct
     2101 tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg gtcagtattt tccacccaca
     2161 ccgccgccag ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta
     2221 accctgacgc gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc
     2281 gtaaaggccg tggttcccgc gctttgcgca gacgttttaa ccttcgcagc caacccactg
     2341 tccgccgcgc cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc
     2401 gccagccaaa acgcggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat
     2461 ccggcaaata cgtttttacc tcgccgggca gatagtcgac caccatccag tcacggagat
     2521 ataaatgcgg cttcggtgca atacatgcgg gtaattgtga taaagcccga tactggcgca
     2581 agaacgcgaa ccgcggcgca tcaggatcgt gcggctgacg tacaacaaaa cgctggccct
     2641 ggtgttcaat gagaaaactc ccgccactaa gcccactatg aaacgtcgtg acgggatgaa
     2701 actgcgggaa aaagcgcgac agcaattcgt cgcgcatgat gggattattg ctgcgaaacg
     2761 gcacctttac ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg
     2821 gtcggagcgt taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg
     2881 cgggcaatgc ctatggcttt actacgagta cctaaactgt cctgcggcga caaacctaac
     2941 tgttgcttag ccatcgacag ctgctgggcg gaaaccaggg taaatttccc gttattagcc
     3001 agcgcatttc gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg
     3061 ttaacgctat caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta
     3121 ctgaccatcg gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt
     3181 gccgtttgat cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc
     3241 actgtgggga caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct
     3301 accggtgcag gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc
     3361 aaggcgtagc gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct
     3421 gactttgtgc gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg
     3481 aatgctaacg ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca
     3541 gtaaaaacga taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc
     3601 tgaaggttga atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa
     3661 taaagatgac tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg
     3721 acagcccacc agcagtaaca acgccagaga caccagccca aagcatcctt ttctcatcgt
     3781 tacagacctt tatgcgccag cattggtccc agcggacggc cacccaacaa gtgcatatgg
     3841 atgtggtaaa cctcttgtcc gccatggcgg ttggtattca tgatcagacg atagccatct
     3901 tcggcaatac cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc
     3961 tgctcatgct cagctgagac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga
     4021 atatgcgttg gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag
     4081 acgatatcgg aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg
     4141 acattttcct ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag
     4201 tatagagtat ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa
     4261 aaaatcgttt tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg
     4321 aaaagacatt ctctgtttgg tttacacgac aaatatgaat gcgtatattt ctcatttgca
     4381 tttacaaaca aaattattcg cacactaaat ataatcgctc atcaccctcc tccggcatga
     4441 gcctgacgac aacataaacc aagggatttc aaatgctttc aacacaattt aacagggata
     4501 atcaatatca agccatcacc aaaccgtcac tacttgccgg ttgcatagca ctggcactat
     4561 taccttctgc cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag
     4621 ccacagctcc agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca
     4681 aaatgcagat gactcaacgt gatattcctc agtcggtcac tattgttagc cagcagcgga
     4741 tggaagatca gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca
     4801 aaagtcaggc ggattccgat cgtgctcttt attattcccg cggattccag atcgataact
     4861 atatggttga tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt
     4921 ctgatatggc actctttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg
     4981 ggacgggtaa tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat
     5041 ttaaaggcga tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt
     5101 tacaaagccc actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga
     5161 ataacgactc atggctggac cgctacaaca gtgaaaagac cttcttctcc ggcattgtcg
     5221 atgctgattt aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg
     5281 ttaatagccc aacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt
     5341 acgatcgcgc acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg
     5401 tctttatgac cctgaagcag cggtttgctg atacctggca agcgacgctg aatgccaccc
     5461 actctgaagt cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg
     5521 atggtatgct ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggca
     5581 ccggttggaa cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtagtt
     5641 atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca
     5701 atcgttactt cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact
     5761 ttaatggcaa tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca
     5821 cacatatgaa atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga
     5881 tcctcggcgc acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa
     5941 accacaccac gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg
     6001 ccagctatac ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg
     6061 ctccaatcac cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc
     6121 tgaccaccac gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta
     6181 cacctatccc cggcagcaac ggcgaaaccg cctataaagc agtggatggg acagtcagta
     6241 aaggggtgga atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg
     6301 caacgcgcta tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca
     6361 ccacggttaa aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg
     6421 gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc
     6481 gcgccgagca aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa
     6541 acttctcgtt acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacgtgg
     6601 aaggttctat cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct
     6661 gaataacacc tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacag
     6721 attagtggtt acggatgtac tcatccatct cggttttcag gttgtcggat ttagtaccga
     6781 aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc
     6841 cagcctgatc cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt
     6901 cgaggttagt aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc
     6961 tggtacctgt cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca
     7021 gtgctttaat cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga
     7081 tcgggaacag ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt
     7141 gcgagaacga cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg
     7201 ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg
     7261 gctcggtgat accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt
     7321 tcgcgcggtt ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt
     7381 acattttgaa caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac
     7441 gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct
     7501 ggaaaggtac gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac
     7561 cgtaaatgcc aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga
     7621 ttgcagaacc aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca
     7681 ggccagaaat gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct
     7741 taatacggta gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga
     7801 gtacgccagt atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca
     7861 gtggcgcaac cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg
     7921 cggatacgcc atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca
     7981 tgtttgcaaa gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc
     8041 agctgaaatt cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg
     8101 gcagcatcag cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg
     8161 agagtgctcc tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc
     8221 ctcgccgtgt acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag
     8281 tgccaatttg tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt
     8341 ttgacaaaac atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttcggcgg
     8401 tgaactttac tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg
     8461 attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg
     8521 ccagttcttc aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca
     8581 tcgcaggttg attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt
     8641 ccaccagtaa tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac
     8701 ggaaggtcac aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg
     8761 tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc
     8821 gcagaattgc cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca
     8881 gttcacgacc aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg
     8941 gtgtgtaata ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca
     9001 gacggcgtaa atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag
     9061 aaaatacgac gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg
     9121 ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat
     9181 ccacgtcctt atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta
     9241 aaaacatgat gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa
     9301 tgctcaatac gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtaacagac
     9361 attaactgtt cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa
     9421 agatggtttg ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagca
     9481 ccatgatggc gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga
     9541 gcttgttcat gattaagggc cgctagcagc gaataccagt cgcccgatgg cacgctatat
     9601 gccaacgcct gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt
     9661 gccgcgccag gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt
     9721 gtcacttcgc gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt
     9781 aaccgacaac gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa
     9841 aaccaggttt ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg
     9901 gttagtaagg cagcatcggt tacccagaca actttcgcac cacctaagcg tgcgtgctca
     9961 ttcagctttt cggtgacctc acgtaccgca tcaatgccca acgcattttt ccctttttcg
    10021 ggagccaggg tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag
    10081 tgaccgcaac ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag
    10141 attaaagcat catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt
    10201 ccggcctgat agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc
    10261 atgcgtccaa ctccttaacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct
    10321 ccagcggctg ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat
    10381 agcgggcgcg ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc
    10441 cgcgcgcacg cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt
    10501 cggggcgaaa atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac
    10561 cacgtcctcc gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg
    10621 tgccgttagc cagagctggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa
    10681 acatcagaac ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca
    10741 ccaggcttct taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt
    10801 cgcggatacc cagttgctca agcgtctcaa ccaccacatt acgcgcggta gttttgcctg
    10861 cgccttccag cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc
    10921 agataatcct gcacagactt gttatggctg gcaagattgg tattaaacgt gtgaccacct
    10981 ttaccatcgg ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc
    11041 gaatccgccc ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta
    11101 tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc
    11161 ccgtaaatca cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat
    11221 accgaggcaa cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc
    11281 atcgtcacca gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg
    11341 ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta
    11401 ttggcggtat acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc
    11461 agttcaagtg cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac
    11521 ggagcctcac gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc
    11581 gggaactgtg cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc
    11641 ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc
    11701 cattgaaaaa cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc
    11761 agacgtccgg tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg
    11821 tcggcaagat ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc
    11881 aataacaaga ttatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg
    11941 ctaaatattc atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca
    12001 tcactggcat taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag
    12061 aagcttgcac ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc
    12121 gcataatgcc gttaacacct gcctgatcca gtcgcggcgt ataaactacg ttgcccttcc
    12181 gccagaacaa attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg
    12241 cctcatcagc gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agacgattga
    12301 gatgtttaat acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg
    12361 tcatcccctc gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc
    12421 gcgttgccgg tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca
    12481 cgactttcag tacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct
    12541 gtggccagaa gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgctggatat
    12601 gcgccgataa caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac
    12661 caaactgcgt tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga
    12721 acataccggc tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt
    12781 cggaataaaa gctaagaaaa aaggcccgca agcggacctt ttataagggt ggaaaatgac
    12841 aacttagatc tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca
    12901 cagcgtgtat tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca
    12961 accttcatcc gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag
    13021 gatggagtag atagattcta ctgcacccgc cgcacctaac agatgaccgg tcatagattt
    13081 cgtggagctt accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc
    13141 ttcagcttta tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact
    13201 cgcttcaatg cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt
    13261 ttctggcggt aacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc
    13321 gtaaattttc gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc
    13381 accatcgccc agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg
    13441 gttatcattg cgggtagata atgcacgtgc cgcgccaaaa ccaccaacac ccagcggcgt
    13501 actggctttc tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg
    13561 cgcagcatga ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg
    13621 gccacgcagg ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg
    13681 aacgaagaat gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc
    13741 gatcagtccg aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc
    13801 ttccgttatt tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg
    13861 aatgaaggca tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc
    13921 ctttactaag ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct
    13981 gatgccactc tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg
    14041 agacaacatg cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg
    14101 agggaaaaaa tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct
    14161 ggagatgttc acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt
    14221 gattttctca gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac
    14281 cagctcaacg gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt
    14341 aacttcttcc tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat
    14401 agtgctcata ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta
    14461 taaaatgttg aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt
    14521 ttgaggcctt accccccaat aacgcaaatc attttgcact aattattact cataaccacg
    14581 cagactgcgc gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc
    14641 gttcacatgc aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac
    14701 cgcgttggcg atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc
    14761 cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac
    14821 aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat
    14881 caagcccgct ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac
    14941 agaaccgata gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt
    15001 tgacagacgg aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt
    15061 cattcgcatt aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc
    15121 accaaattct gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt
    15181 caacatcaga cctttgccgt tagcacctaa ataatcactg atcgcctgag cgccattttc
    15241 actggtcgca gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg
    15301 gccaattccg cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt
    15361 cctcttttaa agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt
    15421 cagggtgtcg acaatgcgtt tcgtcaggcc agttagcact ttgcccgggc cgacttcata
    15481 gagatgttct acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt
    15541 atacaactga cgtaccagtg cgtcacgaat ggcatcacca ttggtttcgc atttcacatc
    15601 aacgttattc acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc
    15661 cagtttgtcg gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg
    15721 cagcgcgcgt tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt
    15781 atgaccggca ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc
    15841 ttctgcagct tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc
    15901 catagcgcca gtgccttccg gtacggcttc ttgcatgaac ttaccgcgca tctcaaccag
    15961 acgcaccgca tcagcgaaat caatcacgcc agcgcaaacc agcgcggagt attcccccag
    16021 gctgtgaccg gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag
    16081 cgcaacagat gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc
    16141 tggcccctgc tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa
    16201 cgtttcttcg acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga
    16261 accctgtcca gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg
    16321 aaccagcgcg gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc
    16381 cggcttaatg cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga
    16441 ggtattaccg tggcgatcca gcgtcaccac gacattgtcc atagacatac cgagtttttt
    16501 cgccgttgca ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga
    16561 acggtcaaga ttatttgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc
    16621 aaccttgaag acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacacg
    16681 gtcagcatta ggcagcgtca gcaactcacc atagctaccg tcggcatgca gatgggtgga
    16741 gatgattccc ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat
    16801 aataatagtc ccacgatcgg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac
    16861 cagagcatac ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt
    16921 gaaacctgcg caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccagcatgct
    16981 ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat
    17041 caggccaatc tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa
    17101 gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg
    17161 agtgacaatc cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac
    17221 ttgttcgggc agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact
    17281 tttcggttat ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca
    17341 gagttccgct tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc
    17401 gagcggcaat tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga
    17461 ccgcaaaagc tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta
    17521 acagacaggc gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct
    17581 tttgtagcca acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag
    17641 atttcagcag agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc
    17701 ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa
    17761 gatagccgat ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac
    17821 tgtcgagacc ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa
    17881 tttccaccac ctcttcagcc aggactgagc ccataatggc aaattgcacc aacattgtgc
    17941 tgtcacaatc gacgttggcc cctaaatcga gaaccaccgt tttgcccttt tgctgatgcg
    18001 gcaataccgt caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg
    18061 ccagccccat cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt
    18121 tcaccaactc cagggccacg cgcattgaac tcccacgact ggcgcggata gcttgcgaag
    18181 gccggacatc actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt
    18241 caaagtcagc tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag
    18301 tgagttgcga attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa
    18361 aatcccctcc catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg
    18421 gtttggtctt tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg
    18481 tgattactta gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg
    18541 caggtgtttt tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc
    18601 atgggaacga cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga
    18661 ccttactcct caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt
    18721 taagctggct aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa
    18781 gaccatgtcc gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa
    18841 ggcgaggatg atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac
    18901 ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact
    18961 aaaacaatac gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag
    19021 cgttaccgtc accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa
    19081 cgacatggag cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac
    19141 ctgatcaggg gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc
    19201 gagagtcagg ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat
    19261 agagtcaaag aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg
    19321 gttactgctt tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca
    19381 tagtttaaaa tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt
    19441 attttagcct ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt
    19501 gaatgtgcag caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg
    19561 gtgcttcgac tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta
    19621 attattggtt cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg
    19681 gaagaaaatg cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact
    19741 ggactggcgt tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac
    19801 gtccatttcc gtcacctgag cgaggcggag attgataatt acgtgcgtaa ggagcatccc
    19861 ctgcactgcg cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta
    19921 gagggacgtg accctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta
    19981 cgtcgggaag ggaaaaaccc gctgatggga tagttgtttg cgccggatgc ggttacgtcg
    20041 catccggcaa tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc
    20101 cattatcagg cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt
    20161 caaaccttca tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa
    20221 cttcaacgct gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg
    20281 tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg
    20341 tgtatgcaca cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc
    20401 aaatgcatag cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac
    20461 acgcacgata cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt
    20521 cacatgcgac tgccactgac cgcgcaccag cgccaggtaa tctttttgca ttcctttttc
    20581 acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc
    20641 tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc
    20701 ccgcaaacct tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga
    20761 aggtttattc agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc
    20821 caccttttgc agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg
    20881 gatacgcacc tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac
    20941 ccgcacttca cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg
    21001 ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt
    21061 tacggatgga gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc
    21121 actcattttt ctatgcttat atttagtttt acaccttatt acttcactac gtgatcactt
    21181 tattgatggt tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg
    21241 ataaatggta aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc
    21301 cgtgtccatc cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc
    21361 atccacgcag caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt
    21421 gcagtcctta ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga
    21481 taattgcgct gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct
    21541 gacgataacc gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa
    21601 aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga
    21661 aaagaatgtt aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc
    21721 agcgtctgta tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct
    21781 acaaaggtaa aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg
    21841 ctgaacgtca cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact
    21901 acagtgctca tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg
    21961 ttcagatcga taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc
    22021 tggcgggtag ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc
    22081 gtatcgaagg cgacgaccgt accgaattaa aagaagcact ggcgagcctt gaactgccgg
    22141 aaggcatggg gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat
    22201 gggatttaag cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc
    22261 cggctccgtt cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact
    22321 tacgtcagga catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc
    22381 agcatatcgc tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg
    22441 agatcccgct gttcagccac taccagatcg agtcgcagat cgaatccgcc ttccagcgtg
    22501 aagtccgtct gccgtccggt ggttccattg ttatcgacag caccgaagcg ttaaccgcca
    22561 tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca
    22621 ccaacctcga agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc
    22681 tgattgttat cgacttcatc gacatgacgc ctgtacgcca ccagcgtgcg gtagaaaacc
    22741 gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct
    22801 ttggcctgct ggaaatgtcc cgtcagcgcc tgagcccatc actgggtgaa tccagtcatc
    22861 acgtttgtcc gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct
    22921 ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca
    22981 ttgttcctgt gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca
    23041 ttgaaactcg tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc
    23101 cgcactacca cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc
    23161 tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta
    23221 agcgtccgga acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa
    23281 cgccagctga acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac
    23341 cagcaactcc tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt
    23401 tcagcggtag tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac
    23461 cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc
    23521 gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta
    23581 atcgtcgcca ggcacagcag cagactgccg agacgcgtga gagccgtcag caagctgagg
    23641 taacggaaaa agcacgtacc accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc
    23701 gccgtaacga tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc
    23761 aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac
    23821 agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg
    23881 caccggtggt tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac
    23941 gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag
    24001 aagagaacaa tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc
    24061 ctcgtcacct gcgcgtaagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa
    24121 cccagtcgcc aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag
    24181 tctggatccg ctatccaatt gtacgtccgc aagatgtaca ggttgaagag cagcgcgaac
    24241 aggaagaagt acatgtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctatcgaac
    24301 cggttgttag cgcgccagtt gttgaagaag tggccggtgt cgtagaagcc cccgttcagg
    24361 ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatc gctgccgcgg
    24421 taactgaaca gccgcaggtg attaccgagt ctgatgttgt cgtagcccag gaagttgcag
    24481 aacaagcaga accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgtcg
    24541 aaactgctga ggttgtagtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtag
    24601 tcgctgaagt cgcagcagaa gttgaaacgg tagctgcggt cgaacctgag gtcaccgttg
    24661 agcataacca cgctaccgcg ccaatgacgc gcgctccagc accggaatat gttccggagg
    24721 caccgcgtca cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag
    24781 gtggtcatac ggcaacacat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt
    24841 aataattagc tcaaagtaat caagccctgg taactgccag ggctttttta tttcatcttt
    24901 gaatcaacat attaaaattc atcgaatggc atccttgcta accaacaatg caaaataggc
    24961 aaaaaaatgc ccgccccagc gatcccagag caggcagaca aaacataccc agtttcatga
    25021 tatgttttaa agcgaaatta tttgttgagc tggaagagcg acaatccctg catatcggta
    25081 aatgctttat acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca
    25141 ttccagtcca catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta
    25201 cctaatgaat ccagcgactc cagttcgttc agctgcgtgc ctaattccgc gcgaacagtc
    25261 agcacattgt tcagcgagtt tttcagtccg cggttggttt tatctaacgc cgcagcggcg
    25321 gtttctttat ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcactatcc
    25381 agcatggcaa aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttgctg
    25441 gtaatactgt cgaaaatttt gtcacccgtg tgccctatca ccatcgaacg cgaagcatcg
    25501 acctgttgtt taatactttc tgctccaccg acgtatttcc ctttctcttc gctaaacggc
    25561 gcagtctctg ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc
    25621 agattcagca actggtcacg aagcccctga atatccgtag ccagcgaggc ccggtcatcg
    25681 tcactcaagg tgccattgct ggcgtagaca attttttcct gagcattctg gatagcagtg
    25741 gtgacctggc taagtacact ctcttccagt gacacttttt gagtggcgaa agtacgtgcc
    25801 agcgtgtact ggctgttttg cgcctgtgcc tgggagagaa caacagcttg tgatgcagca
    25861 atgggatcgt ctgaagggtt aacgactcgc ttacccgtcg acatctgctc gccgtacttc
    25921 atccattctg cctgagaatt ggtgatgccg cgcatgtttt gctggtacat catctgggta
    25981 ctgaaacgca ttttatctcc ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg
    26041 ccgtctgcag aacctgcgca ttcgccaggt aatactgctg aaaacgttgc agatttccgt
    26101 actcctcatc gagattgaca ccggaaatcg actgctgctg attggaaagc tgcgtcacca
    26161 cattaccttg cgtggtgcta ctggttttca acgtcgcggt tttattaccg atatcactca
    26221 ctaacgaggc ataagcgtcg ttaaaggatt tcgcaccgcc caccgtttta ctgttgcttt
    26281 gcagatccag cagggcctga ccgttgcggt tgtcgctatc acccgcatct tcttcgctcg
    26341 ccatcgcgat tttcgcttcg tcggtgatta atacatccat gttgacgatg gcgtcactta
    26401 ctggtttcag cgtgaagctg tcgttaacgg caggcgttcc tgtaaacgtc aactccagac
    26461 catcaaatgc cactttaccg ttggcatctg gtgtcgccgt aaaagtggta ttgctggcaa
    26521 ggcgggtgac ctgccactga ttattatcga acgagatttt gtaatctgtc gccagcacca
    26581 cggaggcatc agttaccgtg gcaccgatcg caacgtcacc tttgtttttc gtgttttgca
    26641 gaaccgcggg cttaccgata gtaaagaaat cttcaccagc atcgccgtta gcatcaaatc
    26701 cggctttgtg ttggctgttg aaagcctcgg caaatgccag cgccagttgt ccaagcgtat
    26761 tacgcgtctg gtccagatcc tgagaacgga atgtcagaat gccgcccagc gacccggtat
    26821 tcagtaattt ctccgggatc tcaatattgc ctgccgtccc atcaacgtaa gcgacagtcg
    26881 tacgagaagg gtcggcgctg gaaggaactg ccgccagttg ccgcgccgta cttccctgaa
    26941 ccagtgagta accattggcc atcgtgatgt tataagtacc gccatcctga acgctgactt
    27001 ctacgccaac aatctggttt aattcgctca ccagttgatc gcgttgatcc agcagattgt
    27061 taggtgacgc ccctgccccc acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa
    27121 tttgtttagc gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt
    27181 tgtcctggtc gcgcagatat tgatcggtgg ttttaaactg attcaccaac ccttctgatt
    27241 tcccaatcag cgcctggcgc gctgccgggt cttccgcgtt actcaccagc gtttgcaggc
    27301 tggtgaagaa atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt
    27361 cgattttcga catctgctca tagcgggcag tcaggccgct actttgcgtc tgcgcagcac
    27421 gtaactggtt ggtaataaac gcatcatact cacgctgcac accagaaacg tagacgccat
    27481 tgccaaccca gccgccagcg cccaacgtgc tattggcctg cgccataata gtggtttggc
    27541 gggtatatcc ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct
    27601 gggccgcgtt cagtccgctc atggcgttat taatcaagct ggacatggag gttccttata
    27661 agccttcaat tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag
    27721 attatcaatg ttcatactgt aggttttgct caccttgtcg cttatcgatt tcatctgctg
    27781 aatcatgttg gtgagtttgc gggcatagtg aggatcggtg gcatagcccg cgtcctgtag
    27841 ggcctgcgcc ccctgttccg cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg
    27901 cgttaacagc ccaacgtaat ccgacaaggc ttccagatac gagctgtaga cgcgaaactt
    27961 cgcttttact ttcttcgctt cgccgttttc atattcagtc gtggtgattt cagtaactgg
    28021 ccctttccag ttgccagagg ctttgacacc aaacaggtta tagctcggct cgccgttttc
    28081 gcggcggatt tgccgttgcc cccaaccaga ttccagtgcc gcctgagcga ggatcaaatg
    28141 atgtggcaca ccgctttgct ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa
    28201 tgctttactg tcacccggca gcgaatcatc gtagttacgt ggcacggcct tttgcaccag
    28261 ctgcgaaagc gcctgatttt gataacgcac cacagtttcg agcgggaatt tcatcggtgc
    28321 tgctggcgtg gactcctctg gcaatggttg ttctggcgtc atctgtttaa ccatcatctc
    28381 tgcaagcccc agacctttgc ccgccgtcat ctgttgggca atttgctggt catacatact
    28441 ggtatacaga cgagtgtgct cgctgctgaa caggccatct tttggtaaag cgtcgcgcat
    28501 gcttttcaac atcatctgca cgaacatccc ttccacctga cgggccaccg gacggatatt
    28561 tgccgccgga tcttcgccca ctttcgcctt tagttcgttg agtgattgcg catcccaggc
    28621 cgcacttgcc agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga
    28681 catcccgcac tttgcattga ttgcaaaata gacatcagat ccatcggcgt agcgcccagc
    28741 gcattgagcg cgcgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa
    28801 ccgccgctct ggcgtaaatc gatctgcgtt tgtggagtaa ccacagtctg tccaccacca
    28861 aacggtgtat ctggctggct gacattggcc tgacgattga ctgttactga gagatttccc
    28921 tgcgctaccg cgcagctgtc gagggtcact tcgcgattca tcaccaccga accggtgcgc
    28981 gagttaatca ctactttagc gtcctgcggg gtgacattaa cctgcatatt ctggatatct
    29041 gcaaggaaac ggacctggga actgttgcca cttggtacgc gcacctgaat agtccgcgca
    29101 tctaacgcgg tggcgctgcc atatccacgc acgcggttga tggtgtcagc gatttgctgc
    29161 gccatgctga aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg
    29221 ctgggcaatt cacgttcaat aaccgcacca ttggtgatcc gtccaccgtt cagttggttc
    29281 acctgaacac tgctaccgcc agcggaggct cctgcgccgc caaccagaat attgccctgc
    29341 gccagcgcat acacctgact gtcaacgccc ttaagcggcg tcatcaacaa cgtacctcca
    29401 cgcaagcttt tggcatttcc catggaagaa accaccacat cgatggtttg cccctgacgt
    29461 ccaaacggag gaagtgacgc tgtcaccatt accgcagcga cgttttttag ctgcatattg
    29521 gtgcccgtcg gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta
    29581 aacggcgtct gggttgtctg gtcaccggtg ccatccagcc ccaccaccag accatagcca
    29641 atcagtgagt tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga
    29701 gccgccgtcg tgaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca
    29761 cttacattgg cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcgt
    29821 taatgtagcc attgcctacg tattcaatgc gcgcatccgc cacctgagta gacggtacgg
    29881 tattgctgcc gctgatagtg cgtggattaa ccacgccaga gaagcgaata aattcggtac
    29941 cctgattaat ggcaatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct
    30001 ggtcaaccgt caccgtcaac gtgccgctaa aggtattgct ggcattggcc ccgccctttc
    30061 cgttgaacgt gttaccaccg gaggcttcga catcggcacg agcgttacca aacagcccct
    30121 gcaaatagcg cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg
    30181 cagaggagct tttgctggcg ctgacgttct cctgcaacac gatggtcagc gtatcgccaa
    30241 tattgcgtgg tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag
    30301 actggaaaat agaaccgttg gcgacgggcg tcggaccggg aaccggttgt gcactggtcg
    30361 ccccctgcac cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca
    30421 agctggaaat ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc
    30481 agtaaaccgg tgaacctgcc accggttaag ccttagagtt gcgtcagttt ttgcagcatc
    30541 tgatcggtgg tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata
    30601 ttgaccagtt cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt
    30661 cccgcgccgt tcaggcccgg cgtgctttcg ttcggtgcac cagaggattg cgtttcggtg
    30721 tagaggtttt cgccaatgct ctccagcccg gtgtcattca taaaggtggt gagattgagc
    30781 tgcccaacct gaaccggagc tgcctggcct tgttgggtta cgctgaccac gccatcacga
    30841 ccgatggtga tacttaacgc attcgccgga atggtgatcg ctggctgcac ctgaaaacca
    30901 ccagccgtca ccagctgccc gttctgatcc acctggaaag agccgtcacg ggtataggct
    30961 gatgaaccat ctggcaacat cacctggaaa aagccctgcc ctttaatcgc gacatctttg
    31021 ctgttgttgg tctgcgacag gtttccctgg ctgtgtaagc gttcagtggc gaccgggcgt
    31081 acccccgtgc cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc
    31141 ggctggcgaa tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg
    31201 ttagtactga cgtttgccag gttgttggca atgacgtcca tattggtttg ctgggcgtca
    31261 aggcccgttt tggcgatcca taatgaactg atcataaaat gtcctgtagt ggattagtga
    31321 caagcagagc agaagcacgc gacagtgtga atttcccctc accctaaccc tctccccaaa
    31381 ggggcgaggg aatagtccgc gcgcttttat tgcaatgtca tcaatcgttc aattaactca
    31441 tcgacagcag ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct
    31501 gcatttcaaa acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt
    31561 tactgccttc cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtatcggcc
    31621 cacgcgtggc ctggctttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgttgcactt
    31681 cgctgcccgt ggctttcacc agtttcagac gccccactgg cgcaaccgta tttgccggat
    31741 cgcccggatt gagcgccgag attgtgccat cggcagcaat agtgatttcc gccccttccg
    31801 gcacagcaat tggcccagcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg
    31861 tgggatcaac ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggactgca
    31921 cggccagcca gccatcctgc tgcaatgcaa cgtccagcgg gcgcgaggtg taatccatct
    31981 tgccgggcgt catgtctgca cccggtgttg acgccgttac cagcgtgcgc gtgggcagtg
    32041 aaagcccttc caccggcact gcacgcaagg cgtttaactg cgcgcgaaaa ccgggcgtag
    32101 aggcattggc cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgctc
    32161 ccatcgcggt ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta
    32221 accagcgtgt tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta
    32281 cgctgggcaa cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccaac
    32341 gcaccgttgg tcagggtgcc aaagttgccc gtcccggctg tccccaacag cgccacgcca
    32401 gaagattgcg tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca
    32461 aagttcgcca gtacaatctg ccccagcagt tgtgtttgtt cgttggaata gttgccgaca
    32521 accgtaccgt catcattgat ttgataactc accagatcgc ccggtttgta gccattctgg
    32581 gtggttgcca caatattgtt agcgccggta ttttgctgca tggagttgag gaagctcagg
    32641 ctaaacgtgg cgggatctgc gccgttaatt gcgccagtgg taatattctc tgttggattt
    32701 gaggtcagaa cgccattggc attaaacacc agcttcattg caggctcggc tgtacctgtt
    32761 ggatcactgc tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaagtag
    32821 acgctcatgt catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta
    32881 tagctatccg cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc
    32941 aggttgatct gcatcgatgc cgtggtggta gttttcgctg ccatcagggt attcgggatc
    33001 gaaatattgg tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa
    33061 cccgtcagct gtaaaccttg catattcacc aggttacggt tttcatccag cttaaattgt
    33121 ccgttacggc tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg
    33181 ctgatagcaa cgtccagacc tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga
    33241 gtgataccgg caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa
    33301 gaggccgtgc ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca
    33361 tcgaggttgg tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac
    33421 tcctgaaatg tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa
    33481 gtgccgagat ccagcgtatt accgctgttg ccgcggatca caccctgcac cagagcaaac
    33541 tgcagcggct gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa
    33601 gaaccgttcg gcgcagtgct gccgtcagtc aacgtaccgt cccaggtgaa actgtgaact
    33661 ccggcggtca gttcaccaat atcaatggtg cgcacaaccg caccattttt atcggtgatg
    33721 gtggccgtaa ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc
    33781 acagctcctt cttcactgcc ggttcccgca agaacagtgg tgccgtggat catcacgccg
    33841 tgaccgatca ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat
    33901 ccgagtgtgg tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac
    33961 gtcagctcgt tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc
    34021 aaagtcagaa aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg
    34081 gtggtactga cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc
    34141 tcctttattg accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt
    34201 caacgttggc ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat
    34261 caacgttcgg catttttacg tagccctttg catctgccag cggattaccc ggttcataaa
    34321 ccagtttgtc cggggcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag
    34381 cacctggtgc agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcag
    34441 gaccggtcac gctatcagca tttgccagat tactggccgc cacgttcagg cgctgggact
    34501 gggcagttaa cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc
    34561 cgctctgtaa aacgttcatc atgcctttga tttgcccgct caacgcgcta aggctcatct
    34621 ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac
    34681 cgtcaagcga aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg
    34741 cctgcgccgg gatgtgttgc gttgaggtca tcgtcagtgc aaccacactg gttgcatccc
    34801 gtccacgttg catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac
    34861 caggggtatc ggcattggcg atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat
    34921 tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc
    34981 aggtatcaaa attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa
    35041 acgcaaaatg ggtcgctatt tatgccgttg atggtcattg cggacaggta caattcacgt
    35101 tgtagaaatg gctgggggcg aaaatgctga caataaaacg tagcgtggcg atcatcgcga
    35161 tactgttcag tccgttaagt acggcgagca atctcacatc gcaattgcac aactttttta
    35221 gcgcccaact cgcgggggta agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc
    35281 tactaccgcc atgcgagcag ccattgcttt cgatgagcaa taattcccgc ctgtggggca
    35341 atgtgaatgt gttggcacgc tgcggtaacg acaaacgata tttacaggtt aatgtgcagg
    35401 ccacaggaaa ttatgtggtt gccgcgatgc ccattgtgcg gggaggaaag ctggaagctg
    35461 gcaatgtcaa actaaaacgc ggacggctgg ataccctgcc accgcgtacg gtgctggata
    35521 tcaatcaact tgttgatgcc attagcctgc gcgatctatc acccgatcaa cctatccagt
    35581 taacccagtt tcgccaggca tggcgggtaa aagcgggaca acgcgtcaat gtgatcgcca
    35641 gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct gaacaatgca gccgtcgcac
    35701 agaatgcgcg ggtgcgcatg gtatcgggac aggtagtcag cggcgttgtt gatgcagatg
    35761 ggaatattct tataaacctg taagctgtta aagattaccc gtcccttgcc gataaataag
    35821 caacacatga taaaagcgcc ctcaatgagg aataaaccat gagtattgat cgcacttcgc
    35881 ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac tgacgcgccg gtaacgaaca
    35941 gccgggcggc aaaaacaacc gcctccacca gcaccagtgt gacgttaagc gacgcgcaag
    36001 caaaactgat gcaacccggc agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac
    36061 tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa aattgccgat gcgctgatca
    36121 acgaagcgca gcaagacttg cagagtaact gatcgtatga cacgtcttgc agagatcctc
    36181 gaccagatgt ccgctgtgct taacgatctc aaaacggtaa tggatcaaga gcagcaacat
    36241 ctctctatgg ggcagatcaa cggcagccag ttgcaatgga ttacagaaca aaaaagctca
    36301 ctgctggcga cgctggatta cctcgaacag ttacgcaaga aagaacccaa tacagcaaat
    36361 agcgttgata ttagtcaacg ctggcaggaa attactgtga aaacgcagca actacgccaa
    36421 atgaatcaac ataacggctg gttactggaa ggacagattg agcgcaatca acaggcgctg
    36481 gaaatgttga aaccgcatca ggaaccgacg ctatatgggg cgaacggtca gacctcaaca
    36541 acccatcgcg gcggtaaaaa gatttcgatc tgaatattat ctccggcctg cactgcaggc
    36601 cggaatgcat tgttacaccg tccggcgggc aaattcttta actttgaagc ccagtaccgc
    36661 cagtgcagcg aagtacgcgg caatccccgc cagcacgacc gccattaaac gcagtaagcg
    36721 ccagggcatg gtacccaatg accactccgg catgatatgt aacataccta aaagcacgcc
    36781 agacatcacc agtaccgcca ccaccagacg caacagaaac gccatccagc cgggttgcgg
    36841 ggtaaagatt ttctgcttac gcaactgcca gtaaagcagc gaagcattca gacacgccgc
    36901 cagaccaata gaaagtgaca gcccggcatg tttcaacgga ccaataaacg ccaggttcat
    36961 caattgcgtt aaaatcagcg taacgatggc aattttcact ggcgttttaa tgtcctggcg
    37021 ggaataaaag ccaggagcca aaactttcac tacaatcagg ccaatcaaac ccaccgagta
    37081 ggcaattaac gcccgctggg tcatcagcgc atcaaaggcg gtaaatttac cgtactggaa
    37141 cagcgaaacg gtcaacggac ctgaaagaat gcccaacgca accgcactcg gcagcgccaa
    37201 caggaaacaa agacgcagcc cccagtccat caaacggttg tattcatcat gattgccact
    37261 ggcaaaactc ttcgacagcg acggcagcaa aatggtgcca agcgccacgc ccagcacacc
    37321 agacggaaac tccattaagc ggtcggcgta atacatccaa gacaccgaac cggaagcaag
    37381 aaacgaggca aaaatggtgt tgatgattaa ggagatctgg ctcacagaga cgccaaggat
    37441 cgccggtccc atctgtttca ccacgcgcat tgctccggca tcgtggaagt taatgcgcgg
    37501 caggaccagc atgccgatct tcttcaggtg cggtagctga tacaccagct gcaggatgcc
    37561 gccgaccgta acagcccacg ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa
    37621 cagcgcgaaa ccaatcatgc tgatgttaag cagtgctgga gcaaacgccg gaatcgagaa
    37681 gcggttccac gtattcagaa tcgctcccac cagcgacgcc agggagatca gcaagatata
    37741 gggaaaggta atctttagta gctggctggt cagggcaaat ttgtcggcag tgtcagcgaa
    37801 gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc atgccagcga ccgtcacaac
    37861 cgccagcgcg agtgtcagca ggccagaaac ataagagaca aagacccgcg tggcgtcttc
    37921 accctgctta cttttatatt ccgccagaat cggtacaaat gcctgggaaa atgccccttc
    37981 ggcaaagata cggcgtaaca agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc
    38041 catccctgcg ccaaagattc tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga
    38101 aaacatggtc atcgagctga cggcggccag cgattttaat aaattcatcg gtgttctaat
    38161 ccagacccac gggcaaaacg cctgcaaggc aggcgctgaa agaggcgaat agtctacctg
    38221 gattatggtg aattgctacc gccagatgtt acagggttta ttcactcatc gcatcgcgcc
    38281 agatcttgtc aacgatacgt tgcgccagca cggcctgttc gccggcggtt tgcggaactg
    38341 tctgattttg cacacattca atgaagtgcc gcgcacagcc gacaaatcca cgttgttcaa
    38401 gagtgctctg ccaaccagga atcggtttat gcactacgcc ctgcccgcgc tcctcacgcc
    38461 attcgcgcat atccgtaatg tcgatgagcg caccgtcagt cacggcctgc acggtttcac
    38521 gctgacttcc ggcacggcga tgcatacagg tggtgatctg caaaggaccg gccgaaaaat
    38581 ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag tagcgtaccg ccatccagag
    38641 aggctttgcc gcccgacaac cacagcgcgg tatccaccac atgcagataa tcatccagca
    38701 acgtgaaata aagatcgtgt ggcccgacac tattgctacg atgtttatcc attcttagcg
    38761 aagctgcggt agcgagttgc gtttttaact caccgtagag tggtgcgaaa cgacggttaa
    38821 aaccgaccat caacgtcagt tttttacgcg ccgccagttc caccagccgt tcagcatcgc
    38881 gcagattttc tgccagcggt ttatcgacac agacatgtac acccgcattg agtaacgtac
    38941 tgaccacgtc aaagtggctg gcggtgctgg aatgcacaaa aaccgcatcg caactggcgg
    39001 caaggctgga taacgaatcg gcataaggaa tgcgccagct ttcacaaatt ggcagggctt
    39061 tcgcgcgcgt aggcgaccag gctccttgta acgtccagtc agacgctgcc gccagcaccg
    39121 gtaaccacgc tttttgcgca atgccaccta atcccactac gccgatacgt aattttttca
    39181 ctttaatctc ccagatgggc cagcaacgaa tcaagacgct gtttcagttc tgccacttcg
    39241 atttccaggg cttcgacgcg ggcctgtaaa tcaccatcaa ccgcgttcga catatccgtc
    39301 accgccggct gatcttcaac ctcaccactg aaaagatgca tgtagcggct ttcgcgttta
    39361 cccagttcgc gggccagacg caccacaaaa ggaccatctt cgcgatttgc cagttgttcc
    39421 agcgtcgact ccacttccgc catatcgctg aattcataca ttcgcgcggc gcagctgcgc
    39481 agttcacctg gcgtctgggc accacgcaat aacaacgtgg tgattaacgc cacttccgct
    39541 gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac gttgctcata tttggtgacc
    39601 cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt tgaccagatt atccagctgt
    39661 tcctgcactt cggattcact cagattcatg accggttcac ggttcgtttt ctgattacag
    39721 gccgtgacta caccattgac tgagagcggg tattgctccg gcgtggtcac ctgcttttcc
    39781 agcaagcaac caatcacgcg agcttcaagg gcagtaagtt gatatttcat gagattctcc
    39841 ttagcggccg ggcgtccagt ctggggtagt taatgccgtc agtacgtgat cgcgccattg
    39901 tccatcaatc aacagatagt ctttcgcata gccttctttt tcaaaaccca gtcgcgccag
    39961 taaatcaccg ctgcgtttat tgtgcggcat ataattagcc ataatgcgat gaatatgttg
    40021 ggtgcgctgc atataacgaa tggctgcggt cagggcttca aacatgagtc ctttgccctg
    40081 ccatttttgc ccaatcgaat aaccgagata gcaggcatga aaagagccac gaacaacatt
    40141 ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg tcgaataagc caaagtagaa
    40201 agctgaacct tgtttatgaa attcgttaat catccccagc ctggcctgcc agcctgatgg
    40261 ataacagtgg ctttcgtcgc gcactggctc ccagggcttg aggaaatggc gattctctgc
    40321 gtaataatcc gcaagacgcc aggcatcacg atcatgcacc agacgcacga ccagtcggtc
    40381 tgtggttaag cgcacttttg gcacgttact gcgatagcca aacattctat acctactcct
    40441 tcccgtaacg tctttaatac gctgtaagta ttactatacc tgcgcaacca gaccctgtga
    40501 aaagtccaaa atgccatttt ctgcattatt cacatatttg ataaaaactc tctatcaaag
    40561 cgacgttttg ataaaaaaat attgtccccg aagtgatggg aaaagtctta gcggcaatgc
    40621 agaatagaaa gtgctcatct tttatttccc gggaggggaa atgtcccgcg tatcgcaggc
    40681 gaggaacctg ggtaaatatt tcctgctcat cgataatatg ctggtcgtgc tggggttctt
    40741 tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa atgggctggg ccgccgtcat
    40801 ggtcggtatt gctctcggtc tacgccagtt tattcagcaa ggtctgggta ttttcggcgg
    40861 cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc
    40921 cgccggattc gccacaatgg gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct
    40981 gctctcggga ctcggcggca cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact
    41041 aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg
    41101 tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa tacgactttc gcctggtctg
    41161 cgccacaggg gccgttttgt ttgtgctgtg tgcggcgttc aatgcgtggt tgttaccggc
    41221 atggaaactc tccaccgtac acacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga
    41281 caagcgtttt gtcacctatg tcctgacgct ggcgggttac tacatgctgg ctgtacaagt
    41341 gatgctgatg ctgccaatta tggtcaacga cgtggctggc gcaccctctg ccgttaaatg
    41401 gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc taccctatcg cccgctggag
    41461 tgaaaagcat tttcgtctgg aacaccggtt gatggctggg ctgttgataa tgtcattaag
    41521 catgatgccg gtgggcatgg tcagcggcct gcaacaactt ttcaccctga tttgtctgtt
    41581 ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga
    41641 cgcaagagct cgcggcagct atatggggtt tagccgtctg ggtctggcga ttggcggcgc
    41701 tattggttat atcggtggtg gctggctgtt tgacctgggc aaatcggcgc accagccaga
    41761 gcttccgtgg atgatgctgg gaattattgg catcttcact ttccttgcgc tgggttggca
    41821 gtttagtcag aaacgcgccg cgcgtcgttt gcttgaacgc gacgcctgat ttgatactcc
    41881 cccctgactc ctccatactg aaagtgaact gacagccaaa tggaggagtc acgtgaagct
    41941 atacatttac gatcactgcc cttactgcct caaagcccgc atgattttcg gcctgaaaaa
    42001 tatccccgtc gaattacatg ttctgctcaa cgacgacgca gaaacaccca cccggatggt
    42061 cggtcaaaaa caggttccca ttctgcaaaa agatgacagc cgctatatgc cagaaagcat
    42121 ggacatcgtt cactatgtcg ataaactcga cggcaaaccg ttactgaccg gcaaacgttc
    42181 ccctgccatt gaagagtggc tgcgcaaggt caatggctac gcaaacaaac tgctgttgcc
    42241 gcgttttgcc aaatcggcat ttgatgagtt ttctactccc gccgcgcgca aatatttcgt
    42301 cgacaagaaa gaggccagcg cgggtaattt agccgacctg ctggcccact ctgacggtct
    42361 gattaagaat atcagcgatg atttgcgtgc gctggacaaa ctgatcgtca aaccgaacgc
    42421 cgtgaatggc gaactttcgg aagatgatat tcagctattt ccgctactgc gtaatctgac
    42481 gctggtagcc ggaatcaact ggccaagccg cgttgctgat taccgcgata atatggcgaa
    42541 acagacacaa atcaatttgt tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc
    42601 ctgtattcgc gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga
    42661 cacatgctgc gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca
    42721 agtttttatt tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca
    42781 cccaatacac catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct
    42841 caaaagatat cggtttaccc ggcgtggctg acgcccatat tgttctgaca aacttgacca
    42901 gccaaattgg tcgcgaagag ccgaataagg ttaccctaac cggagacgcc aatctggaca
    42961 tgaactccct gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg
    43021 tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg
    43081 tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct tccctatttg aaccaggcat
    43141 tacgcaatta ctttaaccag caacctgctt acgtcctgcg cgaagatggc agtcagggcg
    43201 aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc
    43261 cgtttacgga ttaatgacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac
    43321 gtttccgctt atcctttgtg tccggcaaaa acatcccttc agccggagca tagagattaa
    43381 tgactgcacc atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc
    43441 gcgatggcga catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta
    43501 ttgtaatgcc caatctggct ccgcccgtga ccactgttga ggctgccgtg gcgtatcgcc
    43561 agcgtattct tgatgccgta cctgccgggc acaatttcac cccattgatg acctgttatt
    43621 taacagattc gctggatcct aatgagctgg agcgcggatt taacgaaggc gtgttcaccg
    43681 ctgcaaaact ttatccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg
    43741 acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg
    43801 gtgaagtgac acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg
    43861 tgatggaacc tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca
    43921 ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc
    43981 cgcagcatct gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt
    44041 attgtttacc catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca
    44101 gcggttttaa tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag
    44161 agagcagttg cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta
    44221 ccgtctttga agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc
    44281 cgcagttcta tggcttgccg gtcaacgaca cattcatcga actggtacgt gaagagcaac
    44341 aggttgctga aagcatcgca ctgactgatg acaccctggt gccattcctc gccggggaaa
    44401 cggtacgctg gtccgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttatttt
    44461 acctgtataa ataaccagca tattcaacag ggggctatta tgcgaattga agtcaccata
    44521 gcgaaaactt ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc
    44581 cgtattcagt atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg
    44641 aataatttat cggttattgg cgcaacaaaa gaagataaac agcgcattag cgaaattctc
    44701 caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt
    44761 gtttttgccg ggtcgccccg gcttttttat tttacttaca aattttgagt acaaaatttc
    44821 tacacttcct caaaataatt gcacaatatg
//
LOCUS       DAZFYC010000024        44829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFYC010000024 DAZFYC010000000
VERSION     DAZFYC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.024"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1544)
                     /locus_tag="ACHKW5_001532"
     CDS             complement(<1..1544)
                     /locus_tag="ACHKW5_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO7510950.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQGLFILSGENKIDEECFINIVQTA
                     VAINNIGRESNGYILVGVSDTKATADRVKALYGVTPIECNGYYINGIDHEAVIQSKNI
                     DNYFLF"
     gene            complement(1829..2161)
                     /gene="pemK-mt"
                     /locus_tag="ACHKW5_001533"
     CDS             complement(1829..2161)
                     /gene="pemK-mt"
                     /locus_tag="ACHKW5_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFO7510951.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(2163..2420)
                     /gene="pemI"
                     /locus_tag="ACHKW5_001534"
     CDS             complement(2163..2420)
                     /gene="pemI"
                     /locus_tag="ACHKW5_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFO7510952.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2513..3166)
                     /locus_tag="ACHKW5_001535"
     CDS             complement(2513..3166)
                     /locus_tag="ACHKW5_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO7510953.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(4107..4964)
                     /gene="repA"
                     /locus_tag="ACHKW5_001536"
     CDS             complement(4107..4964)
                     /gene="repA"
                     /locus_tag="ACHKW5_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7510954.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENKQRKKQGLDSLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(4957..5004)
                     /locus_tag="ACHKW5_001537"
     CDS             complement(4957..5004)
                     /locus_tag="ACHKW5_001537"
                     /inference="COORDINATES: protein motif:HMM:TIGR03475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510955.1"
                     /translation="MCTLLLRIVSAGWCD"
     gene            4995..5231
                     /locus_tag="ACHKW5_001538"
     CDS             4995..5231
                     /locus_tag="ACHKW5_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012006549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO7510956.1"
                     /translation="MCTERSLELFQAYNYTPRIAELLAIRFLDGPGNLSVLAKVREDY
                     RGFHFSGSCTSTSEPVPCHLMAWPATKFLKRSVI"
     gene            complement(5267..5524)
                     /locus_tag="ACHKW5_001539"
     CDS             complement(5267..5524)
                     /locus_tag="ACHKW5_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO7510957.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAAEGVTQAELIERLIEKEACHRNMA"
     gene            complement(6040..6246)
                     /locus_tag="ACHKW5_001540"
                     /pseudo
     CDS             complement(6040..6246)
                     /locus_tag="ACHKW5_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6438..7169)
                     /gene="traX"
                     /locus_tag="ACHKW5_001541"
     CDS             complement(6438..7169)
                     /gene="traX"
                     /locus_tag="ACHKW5_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFO7510958.1"
                     /translation="MNQGKSVAVQPVRPVLNFSALQRDVIRMVAFVAMVGDHIATAFQ
                     LDMPLLNMVGRCAFPLFVLVCGCNMAGKTLRQHSLNRLWLMALLAQPGYWLAFRDVGL
                     AWWQLNILFTFAVVMQVALFLQSATARNGVAAFTALMGYIPFSSASYGIPGLLMLAGA
                     LLVWQVRDTLRPALFAVWLLLVALLNARHGDVMMFSGVILTLTVLFCVHGLVPASGRR
                     LQAGRWFAPAYAIHLFFIGFLVRVL"
     gene            complement(7166..7732)
                     /locus_tag="ACHKW5_001542"
     CDS             complement(7166..7732)
                     /locus_tag="ACHKW5_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO7510959.1"
                     /translation="MFIAFIIIVILLGGFVLLLRQAGRAAHPLLQSLRSRGIRPGAAE
                     LLLCSRPSFVSAGRLMTEREQRFLRRLDRVTDTRQWRLCPQVRVADIVRVAPDRKSGS
                     REWWQLFRLVSQWHCDVVITDRAGRIIAAVELDDRSHQEPKRQRRDLLLEEVLKQAGI
                     PLLRGDNEQQLAARVRAHLCAQRPEAAA"
     gene            complement(7750..13050)
                     /gene="traI"
                     /locus_tag="ACHKW5_001543"
     CDS             complement(7750..13050)
                     /gene="traI"
                     /locus_tag="ACHKW5_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO7510960.1"
                     /translation="MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEV
                     ASADMDAVRQGKLPDGSDLSRMVNGVNKHRSGYDLTFSAPKSVSVMALVGEDRRFIEA
                     HNRAVAVVMQEVEKLVSARITEEGKTETVLTGNMVAALYNHDTSRDLDPQLHTHALVF
                     NATFAGEKWRSLASDTRMKTGFSENLYATKIALGNLYRSALREDVESMGFETVAAGKN
                     GLWELKDVPVDVFSSRSQTIREAAGPDASAKSRDVAALDTRQAKAWADPELLKADWRR
                     RLADEKFDIDGYIRQAQARVEVPGAVAGGHSGIHAPDLSGGASSGEIADEQVRKAVSD
                     TISALSEKKVQFTWSEMLAGTVNRLPSAPGLFEQARAGIDAAIEGQRLIPLDREKGIF
                     TSDIHLLNELSVHQLARTAIQEQTVLLFPERAQERDIPAGDAVSVLTQDKSPVAILSG
                     RGGAQALRERTEDVAMMARSQGREVMVIAADGRSGQFMSESPHLAGHVMLRSQMNADT
                     VLPVQGTVIVDRAERLSLKETVLLQEKALSAGAQLIFMDTENRQGTGNALSVLKEAGI
                     PQYRFYGTQLPEVRLVSEADKRSRYSQLAQDYVRLSAEGRDVVAQVTGAREQQQLTDI
                     IRESRREAGELGREQMTLQVLEPVWLDSKTRHQRDNYRAGMVMEHWDAEKKTMTRHTI
                     DRVAEATNSLVLQGEDGSRLTLKVSQLDGSWSLYRSRTLTVSEGDRVRALGRELKGAI
                     KAKEQFTVTALENGAIRLRSGDRELRLPTERAVKLAHDYVEGTGAGTSASRTVLAAVG
                     PRSLNKQVLNALAQSGGDIRIYTPLEAGQAARKVENVSSVRLASDQVRQSTGETNLDT
                     AIQASRERLMSDAEQAVNLAIPRAQQGQVYLSEIALLSEAVKSGQPLADVRTEIARQV
                     NSGELIQLDSVSRVGNRVLVPRVAYEMEKSIIRHIAEGKDAVQPLMALTPASVLAGLT
                     AGQREATRTVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRPQVIGLGPTHR
                     AVHEMREAGVEARTLASFLSETRQAIQAGETPDFRNVLFLTDESSMVGNRDMAELYQL
                     VAAGGGRMVSSGDTAQLQAVSTGQPFRLVQQRSAIDTVVMQEIVRQTPALRPAIESII
                     AGQVETSLRQVDDVSPQQVPRQAGAWVPEKSVMEIRKPEKEPEPEQGRTAAQEALKPE
                     QLSPARTDIIAAIRDDWMGRTPEAQQQTLIMAELNADRHAINEAIHVARHEKGDTGAE
                     ERTFTVLEPLRVPDNALRAAETFAEYTGSVAMMNERYWMVADVNPQDGVVTLRNTDGE
                     SVLISPQQNIAQDISLFTHRELTLSQGDRVRFTRSDTDRGYVANSLWEVAGFTQEGAI
                     RFRQGEQEKIVDPQSMTEDRHIDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLE
                     STYVTLSRAKEHVQVYTDNLEDWQQQVRQSDGGKTAHDLLHEKSDRESDTGNRLLATA
                     SRLDKTALGRRVLAENGLEGETMARFIAAGKKYPSPYVALPVWTRHGKEAGALLTEIR
                     IEDDGMRVVLSEESRLRGGEDAQFAGLQASRNGQTLIADSADAALRLAQENPESGVVI
                     RLHGEERLLNAARLTGGRITEPDELSRALRNVADAETAAKAEDPITLPPDEQQKLAEV
                     QEKAARELAEQARRELLSGAPQKDEDKRDLLLSADDERRLRDGTERESRELDEAVREA
                     VAGGRGARQQVREQMQRTEREWVVKMPEKEIEMEKTLGGD"
     gene            complement(13050..15281)
                     /gene="traD"
                     /locus_tag="ACHKW5_001544"
     CDS             complement(13050..15281)
                     /gene="traD"
                     /locus_tag="ACHKW5_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7510961.1"
                     /translation="MSLNPRDLTQGGQVAFMRLKMFLQINNLISYYVIMGTVLFGIAV
                     LLMRISIQNLTNGIIYWFVRMISPFTEKMVSQPVYTIRYYEHKLQYSARQILNDDYTV
                     YCGQLLKQELIIAGCAALLVAFVATFAVYWYIGRTGRQQSEDEIIGGRALSESPKDVA
                     RMMKKRGEASDIRIDDLPLKLDSEIQNMSMHGTVSTGKSTLMRKIMKQLRDRGDLVII
                     YDKGCTFIEDFYDESRDKVLNALDARCPNWDLWEECRTISELENASATLIPASSGEDP
                     FWQGSARTIFAEGAERMRRDEDRSYNKFLRTLLAIQLDQLRTFLAGTPASTLVDGKIE
                     KTAISIRSVLTNYVKAMRYLQGIDRPGREKFTIREWMKGQADKSKNGWLFITSDEQNH
                     ESLKPLISLWLSIAATSLLAMGPNRQRRVWFFYDELASLHKLPTLPRIISEARKFGGC
                     FVLGFQSFAQLEEIYGPKAAAALFDLLNTKFFFRSPSAQIAKFVEEDIGETWRKKFSE
                     QTSFGHEQVRDGISFGKEEERVSIVSYSDVQSLNDLQCFVTLPGSYPVVKLTMKYEAM
                     PKVADSLLLRDVQTSLDQMLEDEITRRTEEERHKFAGLFEGASLIQAVPESNLSNTPP
                     PSAGRVAAVLAETNTPPETRTLQTENAAGDADTKNTGSTAVAAEGGSTSTGGTERDIE
                     QDLPKDVPPGLNSDGEVVDSEAYEDWVQASHQTRDMTRREEVNINHATDKARDFDDDR
                     EVF"
     gene            complement(15425..16162)
                     /gene="traT"
                     /locus_tag="ACHKW5_001545"
     CDS             complement(15425..16162)
                     /gene="traT"
                     /locus_tag="ACHKW5_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO7510962.1"
                     /translation="MKQNIKLMAATAILSSTLVLSGCGAMSTAIKKRNLEVKTQMSET
                     IWLEPSSQKTVYLQIKNTSDKDMSGLQAKVTKALQDKGYTVTSAPDSAYYWIQANVLK
                     ADKMDLRTAQGFLSQGYQGAAMGAALGAGITAYNSNSAGATLGVGLAAGLVGMAADAM
                     VEDINYTMVTDIQISEKTNATVQTDNVAALKQGTSGYKVQTSTQTGNQHKYQTRIVSS
                     ANKVNLKFEEAKPVLEDQLAKSVANIL"
     gene            complement(16337..16894)
                     /locus_tag="ACHKW5_001546"
     CDS             complement(16337..16894)
                     /locus_tag="ACHKW5_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein traS"
                     /protein_id="HFO7510963.1"
                     /translation="MRKSVLEQDVSSLIEKIKESDYEIPKSSDVMKIILKRIQVVIII
                     QLVMILCHFIIYRYDHEALIGISILSAGGILFFSAVFVPSLYQPVSIMLSINEVDKGD
                     SLIVKLLMRKLKQYWKFLVVINLIIGVGLLLSGEGVISGLGFSWFVTYLICMMSFQMS
                     LSRYMTPAVVSSLSKIKELISASPK"
     gene            complement(16904..19795)
                     /gene="traG"
                     /locus_tag="ACHKW5_001547"
     CDS             complement(16904..19795)
                     /gene="traG"
                     /locus_tag="ACHKW5_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO7510964.1"
                     /translation="MAIDTIYTIAGGAWFQDTLNGVASFLDSRAGDALIAMATAVSVI
                     VGAATYIRTRNIMDLVKWAGFYVLVIAVLIGDKRSVQIIDLSEPARIYQVDNVPAGLA
                     APASMITRIGAGMAQTYDTIFARPDALTYSKTGMLFGAQLAAGSSDFRFSEPEIQRMF
                     SDYVHNCVVGDIMLNNKYTIGELMNADDPYALIFSRPSPLRGLYDKNRNFLTCEQAAT
                     RINTDSGDISGANTSPFLQQILNTLHGFTDQVFGQTNGAQSALFTEMLGDSYNYFHRT
                     SLTSTEIIRKNVIMNGLRSGLESFSALSGDTAGLVNVATQTSMAKMRLSQATSASIAA
                     NTLPVMYSVLLGMTIALFPVLIVLAVVSSLSWAIIKGYVYTIAYLQMWPILFSILNHA
                     MNFYLQGKLSGTAVTLANFDQVQNTYSDIGTTAGWLALSIPFIAYGMVKGLGQVVSQA
                     GNYLGQTLQSASTQSSSQTIDGNWHYNNMTTGNVQGNKWDTNYSHREGHMTSQLESGA
                     MKTLTSSGQSVYNTTEAISKLPVDIALGKTATSSWQQQQRDAMSEAQSLTNSLSQTSS
                     LAASQMSQWSQQRGNSDTTVVGSDISNSSNLTKALNTIQSIGNRYARDENVSLAEAVR
                     EAATKSQETSAGGGLQVSFDTDRHLLGKIGGLATGLKGSAEGHVKYAGRSGSSHGTSS
                     DLTRQGGSSKGFSAQELKDFRDAMDVVASSRVTDSGSHTDNESASLASQLSSTFSSLK
                     SQASQYNDAVTRSHEYARLASYAENNSANINQNYSQEFVGYVTSQRPGEADQLLSDTA
                     SPEVRAERERLVQAFVEERIKPQLLQQFEQNKGRTGDGIGNVSAPTVASDLKGEYREQ
                     QADMENSAAQHGVKAEGVVATDVQNQRSNVESQVNDEKNTVISSGESSGKEYRDLQNE
                     HDQGKKAFDDAQKAENERQKFYKNDFVISHDENIKQIKNDMSNRFNGKT"
     gene            complement(19797..21158)
                     /gene="traH"
                     /locus_tag="ACHKW5_001548"
     CDS             complement(19797..21158)
                     /gene="traH"
                     /locus_tag="ACHKW5_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO7510965.1"
                     /translation="MRLKPLCLAFTLGLYSAGTAQAGLQDDMNSFFNNMSYASNSTSA
                     KAWQGQAARYVTGGSFYARTGNKNIQLISISLPSINAGCGGIDVYLGSFSFINSDQIM
                     AFVKQTMANAAGYFFDLALETTVPELKAAKDFLQKMAADLNRFNMSSCQAAKAMVDSV
                     ASLWGESQQNVCQSVAGQNNVFSDWVSSRQGCTSGGKYESVTNKATGAEKDQVLKDIN
                     LMWDALSNSTLSSNAELRQFAMSISGSVIFGSNGEMRILSSLASDRSLLSAMMSGGSA
                     KVYVCDNQNKCLSPSLNNVTISESKSLIRMVEDTLTSIENKAITDTPLTDKEKQFINS
                     TSIPILSWIVDQSSLSVSQSLFAQLTDYIAVDIYLQYLEAVMKVVNGSLATKDYPGAN
                     MNELKNGLADARQALNSLRMEVQIKENALISAQQQIRFIRQQLSSKMSDRVLGNYQFS
                     RVN"
     gene            complement(21164..21766)
                     /gene="trbB"
                     /locus_tag="ACHKW5_001549"
     CDS             complement(21164..21766)
                     /gene="trbB"
                     /locus_tag="ACHKW5_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO7510966.1"
                     /translation="MLILVLRTAILMAAMLSGCLQAGTLDDIRALEAGKNGHSAPALD
                     GLRLPESNTPPSPGLSEKTSPVWYPLSDGRRVDLQDWKLVLFMQSTCQYCRQFAPVLK
                     SLSQQSGLSVFPVSLDGKGDVEFPDVLPATPDVMVEFFQSGVPVATPTTFLTNVNTME
                     TWPLLQGAAEAGEVRKRLDDVFRMTLDRQAGKSLQAHSQE"
     gene            complement(21790..22569)
                     /gene="traF"
                     /locus_tag="ACHKW5_001550"
     CDS             complement(21790..22569)
                     /gene="traF"
                     /locus_tag="ACHKW5_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO7510967.1"
                     /translation="MRAVAGVLVLLATASLHAAPDDVLVPNYAREEGWQWYNPNAEEI
                     QPEPAPEPTLQERLASMTPSEQKKVLQQATREALDTAILYPSAENFRRFMILQNFWTD
                     RATDFTQTSKLARLKYPELDYNVKRSHYNGSVEARLTEERKVQNAVIRQVAQRYGLFF
                     FYRGNNAVDNLMAGVIRAFCEDRGITLMAVSVDGKISDQLPQSRPDSGQAEQMRATHF
                     PATFLVDPKTHQWQPLAWGFMSHDDLDRQMVNVLTHFKPDY"
     gene            complement(22566..24404)
                     /gene="traN"
                     /locus_tag="ACHKW5_001551"
     CDS             complement(22566..24404)
                     /gene="traN"
                     /locus_tag="ACHKW5_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO7510968.1"
                     /translation="MKRYLPLLILAASFCLVQPLVANEQFNQGMNLSSQNKGQGASAI
                     QGFNPAEVIPGYTNSPPESGYYGGVTSPGVDMTAPGSAALNTSEAGKIITESILNTPP
                     DNKPSLDAPFISEGLAMKDKAEAITGGGFDGCVDQVAGFTEITTHQCLRDTKIEQYCT
                     RTATISGYYEDTTEIKQIVIESSSIRFTEKNNSFSGEFKVSTGGKVIAASAVYSWNKK
                     LAYGNEHWFMRVTTPFGLISMDDSEGSVTLSSGLNITDGQSLPFSITNRRNKPTVSSI
                     WYSSNMKYRFTITLTIQVPSRKWVPEVVWTESCPFSKSEGAMTGSQCVEPGGTRTVVV
                     DGKTYSIHQDCWKWQDTYLTQTETEGTCGEYMKNPACTVVRSQCADTVDGFCVSQQVT
                     YSCERKKEGNGQICGGEFFCKDGSCAQAQTGTSNMFGQAVSALAAVAAAGEDVAELNG
                     ENVRAFTGEAKSCKKFAAGFNNCCKDSGWGQDIGLSSCSSDEKALGKAKEKKLTVYVG
                     EYCSKKVLGVCLEKKRGYCVFDSKLARIVQEQGRRGQLGIGFGSGKSPDCRGITVDEL
                     QKLDFGVMNFSDFYDDLNAGSDIPEDQALLKKAQDIIAEKMKENAP"
     gene            complement(24401..25015)
                     /gene="trbC"
                     /locus_tag="ACHKW5_001552"
     CDS             complement(24401..25015)
                     /gene="trbC"
                     /locus_tag="ACHKW5_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO7510969.1"
                     /translation="MKRVMVSLALLLVSGGGMANNHTENRQFIHEQLQLDRQRFQSLQ
                     TPEFLKQTRPVAPQEQSFLDAQAQQFRQSMQPGERPVNAALVFVSFSMPAEELKQRVK
                     DAAQLNIPVVIRGMVNGDMRATANAVAGLVKESNTGGVQIDPTTFRKYNITAVPVLVV
                     ACGNQGEKVDRLQGDLTLHQALKRVAEEGDCADTARSLLGEGGE"
     gene            complement(25012..25380)
                     /locus_tag="ACHKW5_001553"
     CDS             complement(25012..25380)
                     /locus_tag="ACHKW5_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510970.1"
                     /translation="MLVSKKKYDFLLARYEQAASRADLLERQQAELNGALKKNGGPFK
                     CILECREAAIALSSSGSEQVWLTLERLAFVDRWISGLLPVLRRQMSVRERRMWEEMLE
                     IRSADRAYGMAHRPSQEETR"
     gene            complement(25391..26377)
                     /gene="traU"
                     /locus_tag="ACHKW5_001554"
     CDS             complement(25391..26377)
                     /gene="traU"
                     /locus_tag="ACHKW5_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO7510971.1"
                     /translation="MNRYVTGLALMLLCLTGTARAASAECEGNFVNPITDICWECIFP
                     VTIGNVPVAKGRQPDTPNPSMPIQFCPMGILYRVGLAIGYWEPMALTDVTRSPYCMVN
                     LGGFNINIGNVGSGSGGQESRAKPGAFYHVHWYKYPLTYWLNIITSMGCLQGGDLDIG
                     YLSELDPMWDSSSLSMIIQPEAILFGNIIAQGACAADAVASLVSKPIDALFWCAGSQG
                     SMYPFNGYVSNEFSPLQTSLLLSERMAFKLHRQGMIMNSIGENRAVCFEYPSPIIPKS
                     RWRYHMVNKYPDAGQCHPLGRSVTRWEAGKNMPNDRRNFGYLFWRKRNCVFL"
     gene            complement(26374..27009)
                     /gene="traW"
                     /locus_tag="ACHKW5_001555"
     CDS             complement(26374..27009)
                     /gene="traW"
                     /locus_tag="ACHKW5_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO7510972.1"
                     /translation="MYRRLLILLALSGMVQAKNLGTWGEMYPIAEQDMLTTIQTRLKA
                     MEASGEMAREQEAFKQRVIENTLRPKPVEGLTLAQENTTHYIDPSLTVSEDLKDHQGR
                     VFARKGQVINPLDTVPFTDTLYFIDADNPQQLAWMKKQNPGTLTYRVILVNGDVREAT
                     NALNTRIYFDQDGSLSRKFELKTIPARVTLAEDRRRLRVDSFALDVPEVKP"
     gene            complement(27009..27473)
                     /gene="trbI"
                     /locus_tag="ACHKW5_001556"
     CDS             complement(27009..27473)
                     /gene="trbI"
                     /locus_tag="ACHKW5_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO7510973.1"
                     /translation="MTTEQNLNSAPSESSVSAESVRKGGNINVKGSRREQRRRRCLRN
                     LLLVAATVLCVNAAVTSLLISWRTPTVVSFDMKGTVDQFTDQAGAQSLNEAQMSVLTE
                     RFMQTLSTQLQEYQRDHNVLILVTPAVVSGAADITGEIQSAVAQKMAAGGGQ"
     gene            complement(27470..30061)
                     /gene="traC"
                     /locus_tag="ACHKW5_001557"
     CDS             complement(27470..30061)
                     /gene="traC"
                     /locus_tag="ACHKW5_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO7510974.1"
                     /translation="MKKFLSVLENLTNAFRMPDGASEMTRNLASLDFPQLTSVLPYRD
                     YDRDSGLFVNRSTVGFLLRAEPLIGANEQIVYVLEDLIKSKLPRKTPISFHLVSSKLI
                     GEQLDRGLSEFRWQGKRADKFNRITRAFYRRAAQQQFNSPTGLPLTLRDYRLYISYCV
                     KAKKRTAAVMTEVAHTLKVLRSSLDAAKIFTQTADAQELATLVHGMLNARHDQLYPDD
                     VRVGKFDELHQRCIDQRIGIDVRPDDVRISLADRQGSVTTTRAMNFMLENNPDMFMLW
                     MGGDNLSNLLSPDLAIASPFVITMVMEAEDQVATQGEATRKYLDRDKKAKSPYATLFP
                     GVAKQAQEWKEIRERLNSNQTCIVRYYYNITTFCPDTDEDALVCEQQVINTFKKNGIE
                     LFSPTYMQFRNWIAMIPFIHAEGLWEDIKMGGATCRAESIQAVNLLPVVADNRLCQGG
                     LLTPSYRNQLAFLDIYGDGMGNTNYNMAVTGTSGAGKTGLVQPILRSVLDSGGIAWVF
                     DMGDGYKSFCENVGGTYLDGKSLKFNPFANISNINESGERIRDQLAVLASPNGTLDEV
                     HHSLLLRGVQEAWEEHEETARIDHVVHKLTAIREHDKYQNSPGITNRLDEIIELLGKY
                     CSWGIYGEYFNSDEPSLTDDARFIVLELGGLQDKPDLLVAVMFSLIIYIEDKMYRTPR
                     SLKKCCTIDEGWKLLNFKNEKVGEFIETGYRTVRRHRGAFITISQNIKDFDADDASGA
                     AKAAWGNSAFKVVLKQDASEFKQYNQNRPNQFSELERSVISKFGDAKDQWFSSFMLRI
                     NDTCSFHRLFVDPLSRAMFSSKGDDFEFIRQCREQNMDIHDAVYLLAQRNFPDEMREL
                     EALAEAA"
     gene            complement(30072..30671)
                     /gene="traV"
                     /locus_tag="ACHKW5_001558"
     CDS             complement(30072..30671)
                     /gene="traV"
                     /locus_tag="ACHKW5_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO7510975.1"
                     /translation="MKKITFLLAGSVLLLTGCAGVKSSFDCDATTSDTCMTMTKANQL
                     ARDKAAKQAGKPAAGGLPSLVNLPATSAAVLPSASRYAVSTSSRIQVTSNKPAVSVDG
                     ATGKSTNAAAPTLTPRLVAGTPVTPVSRPAVSVGNPAPSCQTVRCDNPGSVHPQRSRD
                     RIATVWIAPWVDSDNAFHQPGRVSFVVSPADWVLPARIN"
     gene            complement(30731..31141)
                     /locus_tag="ACHKW5_001559"
     CDS             complement(30731..31141)
                     /locus_tag="ACHKW5_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510976.1"
                     /translation="MTTNSNGAPGNTRHLNDIAVCCENPGMRVICVDDAESDFWLSAS
                     LPCATVLEGKLRISQDVLVLQRERGHCLFPGTPGAGMSITKQPMYRFFLAKSDVSQIK
                     ALYSGEDGERLAEVIRQNCNVWYPLKPGDGHHDK"
     gene            complement(31125..31310)
                     /locus_tag="ACHKW5_001560"
     CDS             complement(31125..31310)
                     /locus_tag="ACHKW5_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7510977.1"
                     /translation="MKNRKTNVMVVGKTGSGKTVISVDVMRTFLSEQQLRDWRELRKN
                     IQKDGKSAGDKDHDNQQ"
     gene            complement(31314..31538)
                     /locus_tag="ACHKW5_001561"
     CDS             complement(31314..31538)
                     /locus_tag="ACHKW5_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFO7510978.1"
                     /translation="MADVLDHLQKQEDLLNRLHIQAARQLIIRGGESLCECECCGNDI
                     PLKRQQTIPGVRTCTECQRVLEIRQKHYLR"
     gene            complement(31650..33014)
                     /gene="traB"
                     /locus_tag="ACHKW5_001562"
     CDS             complement(31650..33014)
                     /gene="traB"
                     /locus_tag="ACHKW5_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO7510979.1"
                     /translation="MANVNKIVRRRQLALFIALVLGIGAGGAGTWMVSEMNLKKAPPA
                     KAPKGEPAPDMTGVVNQSFDNKVQRSAIAEAQRLNKETQAEIKKLRTEMGIIGRDLKG
                     SQERIRELEDENKLLQTQIEAGKNFDSLSAEPLPGALASQGKAAVQAGEVLPPTTFWP
                     AGGGQAPVAPVMTPVQRPGMMDSQEFTLPDTTPQKPRFPWISSGSFAEAIVVEGADAN
                     ASVTGDKNTVPMQLRLTGKVQMPNDQEFDLTGCFVTLEAWGDVSSERAIVRTRSISCK
                     LGDDNIDQKIAGHVSFMGKNGIKGEVVMRNGQILLYAGGSGFLDGIGKGIEKASSTTV
                     GVGATASMSAGEIGQAGLGGGVSSAAKTLSDYYIKRAEQYHPVIPIGAGNEVTLVFQD
                     GFQLETLEEARAKAAARRKQNQPSASSAPAAMPGNTTDMLKHLQDFRLGDTVDPATGQ
                     VVTQ"
     gene            complement(33001..33741)
                     /gene="traK"
                     /locus_tag="ACHKW5_001563"
     CDS             complement(33001..33741)
                     /gene="traK"
                     /locus_tag="ACHKW5_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO7510980.1"
                     /translation="MKNNFPALLLGTALMITSPVTVYAQSPATLSFPQGGQFRLSISN
                     TDPNMIFIPGDKVTAITAQNGMLADKRLTSAGGVLFTSVATRAFTLYVETALGQTFSV
                     VATPVKGEGRVYRLMSAEPPSRPETRHWETAQAYEKLLIALNRAVLTDTIPDGYGEVK
                     PLADGINVPGGFSATPQKAWSGDKLRVDSYELRNANTWGVALREQDFWKPGVRAVMFD
                     SNAQTLMGGGRLTVTVIRENGEGTDGQR"
     gene            complement(33731..34294)
                     /gene="traE"
                     /locus_tag="ACHKW5_001564"
     CDS             complement(33731..34294)
                     /gene="traE"
                     /locus_tag="ACHKW5_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO7510981.1"
                     /translation="MEISARNSSTKIIAIIILSLTALVFILGFAVTWLAVTNRTLIHE
                     QRTVVTPMTYNAPFAVSETKADTEFFRMMTLSFLALRLNVSPETVDSNHAFLMSFVEP
                     EVREEFKKVLQEEAAQIKASDVNSTFYTTEINVYPVDGRVDVRGVLKMWIGNSKPTTE
                     LKNYRLRLKYAGGFTRIGRFYEVTNEK"
     gene            complement(34314..34619)
                     /gene="traL"
                     /locus_tag="ACHKW5_001565"
     CDS             complement(34314..34619)
                     /gene="traL"
                     /locus_tag="ACHKW5_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO7510982.1"
                     /translation="MSGNDLEKFRFPKTLSEQNRIIGLPLDEAIPAAVLVLWGFFAKK
                     YVFGLIIATVVWQLIRAAKRGKSSRWLYNWCYWHLPVELFRVVYRVIPDSSYRKWIK"
     gene            complement(34621..34980)
                     /gene="traA"
                     /locus_tag="ACHKW5_001566"
     CDS             complement(34621..34980)
                     /gene="traA"
                     /locus_tag="ACHKW5_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO7510983.1"
                     /translation="MNLSLSEGGRPASVKNRAWHYCQMAWRGVCTPKALSRLAAFSPL
                     LLLGMGQMAHATDLLAGGKNDVKATFGADSFVIMCIYIAEIVIGVATYIRTKNLLILL
                     GLVVVIVFTTVGLTFVN"
     gene            complement(35077..35244)
                     /locus_tag="ACHKW5_001567"
     CDS             complement(35077..35244)
                     /locus_tag="ACHKW5_001567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510984.1"
                     /translation="MTKNIPLKPRPAGKFIRVYVDGIVYEELRKIAKKDSRNIPKTAA
                     LKIEEALGMKE"
     gene            complement(35460..35741)
                     /locus_tag="ACHKW5_001568"
     CDS             complement(35460..35741)
                     /locus_tag="ACHKW5_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFO7510985.1"
                     /translation="MAIIDPIRMEILRKVFPEINDIKIEVFTLFAFGMTIREISVYRN
                     TTHQAVYKTLKELCDQYNSPSNEALKTLYITRLALHSFLELRIELTPEQ"
     gene            complement(36220..36606)
                     /gene="traM"
                     /locus_tag="ACHKW5_001569"
     CDS             complement(36220..36606)
                     /gene="traM"
                     /locus_tag="ACHKW5_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO7510986.1"
                     /translation="MPRIQTFVSNQVREEIENLVTERRQEGATENDATVSSVTSMLIE
                     LGLRVYRIQREKKESGFNQMEYNKLMLDNISRVRFMCSEIMNMSMLNQESVASGYFTI
                     EKIKPAMEKFVNEQVGIFFHDDEETD"
     gene            37078..37530
                     /locus_tag="ACHKW5_001570"
     CDS             37078..37530
                     /locus_tag="ACHKW5_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7510987.1"
                     /translation="MTKLLLIGLLTLSSHVIAQDCFELAGRDYKIDPDLLRAISWHES
                     RNRVNAIGINPVTGYGSGLMQIDSQHFKELSRYGIEPEHLVTDPCMNIYTGAYYLAIA
                     FKKWGVTWRAVGAYNAGFRNTEEQDRRRKTYAEKIQNIYRNIKNMQGQ"
     gene            37637..37795
                     /locus_tag="ACHKW5_001571"
                     /pseudo
     CDS             37637..37795
                     /locus_tag="ACHKW5_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(37956..38171)
                     /locus_tag="ACHKW5_001572"
     CDS             complement(37956..38171)
                     /locus_tag="ACHKW5_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510988.1"
                     /translation="MEQCIFDFGIINYNPAITWTVPAVFFFLALLAGIVFNIGRKNNL
                     IVFVWAISLPVITVFIFIFLGGKINIC"
     gene            complement(38512..38754)
                     /locus_tag="ACHKW5_001573"
     CDS             complement(38512..38754)
                     /locus_tag="ACHKW5_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7510989.1"
                     /translation="MGYITYVTDQRPGESDILSGNAFADLEIRDSDGHILLKMPAPES
                     GWTHESLSLVQPQKVRDGEEAFDAYLNGMWIGSTEV"
     gene            complement(38796..39076)
                     /locus_tag="ACHKW5_001574"
                     /pseudo
     CDS             complement(38796..39076)
                     /locus_tag="ACHKW5_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107523.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            39377..39755
                     /locus_tag="ACHKW5_001575"
                     /pseudo
     CDS             39377..39755
                     /locus_tag="ACHKW5_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39993..40115)
                     /locus_tag="ACHKW5_001576"
     CDS             complement(39993..40115)
                     /locus_tag="ACHKW5_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7510990.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(40060..40212)
                     /locus_tag="ACHKW5_001577"
                     /pseudo
     CDS             complement(40060..40212)
                     /locus_tag="ACHKW5_001577"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(40362..40676)
                     /locus_tag="ACHKW5_001578"
     CDS             complement(40362..40676)
                     /locus_tag="ACHKW5_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFO7510991.1"
                     /translation="MKWRYSLRWKLPYPCPGERELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            complement(40673..41392)
                     /locus_tag="ACHKW5_001579"
     CDS             complement(40673..41392)
                     /locus_tag="ACHKW5_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7510992.1"
                     /translation="MTARQNALVPLNAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     EQLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVTAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(41389..41823)
                     /gene="psiB"
                     /locus_tag="ACHKW5_001580"
     CDS             complement(41389..41823)
                     /gene="psiB"
                     /locus_tag="ACHKW5_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7510993.1"
                     /translation="MKTELTLNVLQTMSVQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFSPFHERFHIVVCSPGEASATWMVILVSISGRPFRVI
                     RTQPSFRPETVNHILRLAASLDEAGYSTASIINLLVTEGGAA"
     gene            complement(41878..43845)
                     /locus_tag="ACHKW5_001581"
     CDS             complement(41878..43845)
                     /locus_tag="ACHKW5_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7510994.1"
                     /translation="MSVTEFKAKTGRKSSRKSSKEQENALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDAERTEQVRKTDAVEEISLPLLTKMSSERTLAVQAALIKQPE
                     KTVALMVWRLCCCVFDYCNTTLHPFGMHIEEYHRRLTSDAPSGKRGQAWLSLMQEKAR
                     LEALLPEGWQKDFTTFFTLDGQTLMNLMVFCTACSVDGVQTREHGHTSRSPLDSLESA
                     IGFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGVARDAEKMKKGDAAEHAEFHMK
                     DNRWVPGWMCTPRPQAETETTEYRDNQADAA"
     gene            complement(43906..44139)
                     /locus_tag="ACHKW5_001582"
     CDS             complement(43906..44139)
                     /locus_tag="ACHKW5_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO7510995.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNRYIRDFGILK"
     gene            complement(44196..44723)
                     /locus_tag="ACHKW5_001583"
     CDS             complement(44196..44723)
                     /locus_tag="ACHKW5_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7510996.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGDDYGFSDDIPF"
     gene            complement(44749..>44829)
                     /locus_tag="ACHKW5_001584"
     CDS             complement(44749..>44829)
                     /locus_tag="ACHKW5_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7510997.1"
                     /translation="NTGGNKRQVPAKRQNRGSRNSKDDGG"
BASE COUNT        10420 a        12592 c        10869 g        10948 t
ORIGIN      
        1 atgaacagaa agtaattatc tatgtttttt gattgaatta cagcttcatg atcaattcca
       61 ttgatataat agccattgca ctcaataggg gtaacaccat ataaagcctt aactctgtct
      121 gccgtagctt tggtatcgga aacaccaact aaaatataac cattagattc tctaccgatg
      181 ttatttatgg cgacagccgt ttggactata ttaatgaaac attcttcgtc tattttattc
      241 tcgcctgaga gaataaataa tccctgttta aaatcataat ttggttgttc tgttcttgag
      301 ttattaagta gagttcttat cagtgtttgc caagcatgat tgataaattt attttctgat
      361 tctttaaaat atcgtctgat taaagcacat aaatcttcga cacttttctg acgagcgcta
      421 gctgcccaac ggcccccttc ggtttgcacc attgaacgtt gtacactgtc tcctaactga
      481 gcgataaatt tctgttcatc gtcaagttgc atgttttcat ttatgattaa ttcatacaaa
      541 gcaagaaaaa cagcttgata gtaacgagga cattcttgag atgaagatgc attgcccaga
      601 atgtgtgatc taaagttcaa attatttgca tcaaataatt tttgtatcat ctcataaacg
      661 aagaggtaat tattgacaat tttatctgca ccatttgtct gaatataatt ctctaattga
      721 gttcgtgtat ggattgcatg actcgtcgaa ccttcaccgt aaaatgtatc gagcgatgtt
      781 gatcctgatg ttggcttatc atctaaaaat atatagccca agatatttgc aactaactct
      841 tcatcgtcag agtctctaat gctaggccta ctaatgatat gatttctaat atagaaatga
      901 ttgtctatat ttattccata atcgagtcca tcattgcaaa ttgatatgtt atgtatttta
      961 ttcagtccca ttatatcgct atgtgtagta tctcctctta taattgtcga tattttcctt
     1021 actaactctg aaaaattact aacgcacccc gcacttcgta gttcttgttt tgataaaatt
     1081 ttaccattgg aatttatgcg tcgaaacact tcgtcaatgt ggtcctctgt tgaagagtat
     1141 tcagatatag caaatctata gcgagcaatt aatgtagaac tttcgcggga tagcactggc
     1201 tctttttgtt gaagtttacc actgtctttt agttgttttg ttaatgcaat ggcatctaaa
     1261 ttaaagtatt taccatttaa tgaaaatcgc tgctcaataa aactcattaa agcttctagt
     1321 ctttgcattc catcaaggat ttctcttttg ttgtcaatgg aaacaaacaa tagcaaaggg
     1381 atagggtatt gctgagtcat tgatgaaatc agtgctgttt tctcttcgag ggaccatact
     1441 aattttcttt ggtatcttcg atttataatt aatttatcat ccagatacca ctcgtataat
     1501 tcttctgctg tcttggagtc aatatctaag ttttcattag ccattagatg tatctcgagt
     1561 atttagttaa gaaactgata ggtcagtatt tgcatcaatg ttcattgaat ttgtatccga
     1621 agtcaatctg atgtaggaac ttcaaaactg cgcaaaacac gcaaatacgc acaagaaagt
     1681 agggctgcgc ttggaattcg gaaaataaac cacatcaaaa ctacttttta gccaaataac
     1741 gtttgggaat caccagaatg gtgggacaac agcggtttta gtgccctaaa tcgtacgttt
     1801 tcatccagtt gcccctcaaa ccccatgttc aagtcagaat agtggacagg cggccaagaa
     1861 cttcgttcat gatagtctcc ggaacccgtt cgagtcgttt tccgccccgt gctttcatat
     1921 caattgtccg gggttgatcg caacgtacaa cacctgtggt acgtatgcca acaccatcca
     1981 acgacaccgc aaagccggca gtgcgggcaa aattgcctcc gctggttacg ggcacaacaa
     2041 caggcaggcg ggtcacgcga ttaaaggccg ccggtgtgac aatcagcacc ggccgcgttc
     2101 cctgctgctc atgacctgcg gtaggatcaa gcgagacaag ccagatttcc cctctttcca
     2161 tgtcagattt cctcctgacc agtcgccggt gcatccagcc attctcgttc ttcagctgat
     2221 atttcagcat tcggatcaca ctgtgccagt agctcagcca gtgaatattg cgggcgtctg
     2281 tacggctcaa caatcagccg gccattatca atgaccatgc caacttcatt atctgtaccc
     2341 agagacagcg cattcagcag tgccggtggg acggtcagca taactgagcc gccaaccctc
     2401 ttcagtcggg tggtatgcat ttttcacctc cataaaagtt atatttaaat ataacatcca
     2461 ctaaaaaaac acaccaggct ttaacgcaca atgtttaata aaaatataac tttcaaccaa
     2521 acagtaaacc cagcgtggtt gcttccatat gcagcaatac cggcagcagc aggccaccac
     2581 ttctgatcct ggccactgat gtaatcaacc ccaccaggaa cagttctgcc agtgtcagca
     2641 ggttctgata ctggctgtgc gcggcgacga acaacaacga cgttatcagc gcccccagcc
     2701 acatcgtcca gcagtaccgt gaacggaaga cgttcagcat aatcccccgg aacagcgttt
     2761 cctcattcaa cggggcaagg ataaagatgg tcagcaacgt caggatcacg tcaggtatgg
     2821 acttatcggc aaaaagtttc gtcataaatg gctcagcagg cagagccagc gccttaccga
     2881 gcagaaatac accgacatac accacggcca tcgcaccgac cagccacggt acgccaacgt
     2941 tgcgcagctg accaacgacc ggtagcggag ctatccaacg gcggtatacc aggaaaacac
     3001 acagcaggta catcagaaca gtaccatgac tgaagaacaa atagtttttt cctgatccat
     3061 aaagcagaac ggcctgctcc atgacaaatc tggctcccca actaatgccc catgcagcca
     3121 gcataaccag cataaactgc agatattgat tacgtgtttg aatcattgca tcgcctgtaa
     3181 atttttaact tgtcctattt ttgtcattac cacggatata cacatgtata acaattcaga
     3241 tatcgttacc aggatatgcc gcatcagcgg catggaaggc ggcactctgt tgtttcatat
     3301 gatacaggag taaaaccgcc gaagcccggc gtaagccggt actgattgat agatttcacc
     3361 ttacccatcc ccagccctgc cagaccatac ccgctttcag ccatgagaga gcttctgtgc
     3421 gcggtcggag tggtcccgac gagggtttac ccgaagtcgg ggcgtatctc cgcgttagcg
     3481 ggccgtgagg gccgcttacg agcgtgtact caatacttcc agcgagaaga ctgacagcga
     3541 tgacggagta tagttacaac attcataatt aaaagcgact ctgttccggc cctttgggcc
     3601 ggggcggcgg cgcttttcag ttatgaggga ggggctttgt ggcttcagtt ctgcgcagga
     3661 caggggtttt tctggtggtt gtgtctgagt gttgtaacta aagtggctcc ggttggggcc
     3721 cgccgcttgc ggtgggaggt gcatatctgt ctgtccacag gacaggcagt gaataggttt
     3781 tctttttaaa tgaatgtaat taagtagttt aaaggagata taaacaggtg tttaaaagat
     3841 acattgcacc ctgtaagact ggcggctggc gctttatgac atgaacggtt gtaaccttat
     3901 ggggaagtcc cttgcagttt aatgtggata agcacaatta cccgtctgtg aggcgtattt
     3961 tgtattaaaa acagggggaa ccggatgcac cagaaggtgg atggtgaggt tgttttttgt
     4021 atgtggtgct gattttttgt gcactgacgg gcttcaggcg tgcgaatgcc tccggcgtgg
     4081 gccggattat tctgaggagg tcactttcag ggagaagctg tggccagccg gctgtaatta
     4141 cggttacgtg acagaatcat gcgctccttc acacgacgct ccacttcgcg ttttaccgcc
     4201 tcaccattag cagtgaagcg cccttccgag atttcacgag tcagctgccg tttcaccagg
     4261 gtgacgatat cctgacgttc cctgttcgca tcacgacgcg cacgggcacg ttttattcca
     4321 cgggacttaa gctctgtctg gtaactgcgg aaacgctcac gcacaaaacg ccaggctttc
     4381 gctatcagct catccatacc cagggaatcc agcccctgct ttttgcgctg tttgttttcc
     4441 cattcaacac gactgcggcg cgcagctgcc actgcatcct cagacacatc aagggcagca
     4501 aacagagcca gtgtgaacgt gatgtcggtc ggaatgtagc acccgataag cgggtcatat
     4561 tccgtctggt aggtaatcaa ccccagctct gacaggaacg tcagggcacg ggtggcacgg
     4621 gtgatggaga gttttccggc agcagactcc gtcgccagtc cgcactcaat ggccagcgtg
     4681 gtaatggagc actggacgcg gttggccagc gggtcatagt ggaaacacag cccctgcagg
     4741 agcgcatcaa tagcccgtcg acgcagcacc ggtggcatgc gccgacgcag accacgcgaa
     4801 cgggcatgcg ctacatgaat ggcgaaatca aaacgggagg tgaagcccac cgccttttcc
     4861 atcagttttt cgcagaactt cagtgttccg gcacctttac ggggtgtaaa caccggatta
     4921 gggttcttta cctggcggta atacgtttgt tgaagatcag tcacaccatc ctgcacttac
     4981 aatgcgcaga aggagtgtgc acagaaagaa gtcttgaact tttccaggca tataactata
     5041 ctccccgcat agctgaattg ttggctatac ggtttttaga tggccccggt aatctttctg
     5101 tactcgccaa agttagagaa gattatcggg gttttcactt ttctggctcc tgtacatcca
     5161 catcagaacc agttccatgc caccttatgg cgtggccagc cacaaaattc cttaaacgat
     5221 cagtaatcta gcactgacag aaaaaactag caagaacgtg atcccatcac gccatattcc
     5281 ggtgacaggc ctctttctcg ataagccttt ctatcaactc tgcctgagtt acaccttccg
     5341 ctgcacacag actggtaagg tagtttttca gttctggtct gacgaaaacc ttgatctctt
     5401 tacatacaga tcgcttgcgc gcagaagctg ctctctgtcg ctctgcctca ctcaaaggtt
     5461 taccctttct gtatgctcgt tttgcgccag atgaggaagt cactgcattt tctgtctgcg
     5521 acatctcgcc tcctcaattc tcaaacaggg atcgtttcgc agaggatact acagtttttt
     5581 gaaatcagcg acttgaaagt tgtgacgatg atctgtgttg gcgcacaaat catgtctgat
     5641 tacgaccgtt taatgtgatt ttgataatga aatgacgaaa aaacagaaaa tacataaacc
     5701 tgtgtgccga ccccgggcgg gagcggcggt ttctggtgca acaaatcaaa accagaaaaa
     5761 cactgacaat ttcttatgaa tcctcagcac aattaaaaaa aaccaatatt aatatcggac
     5821 tttccatatt aatttatatt gttgacatta tgtttgtata tatgacaaat gccattcact
     5881 aaataatatc gaaacgccgg aagcggggaa aacttgcttt ttcccgtttc cggggttgga
     5941 caactgagca acgcgaaggc gtcagcccac ctgacttgta tgttctttgg ttttcgccag
     6001 gcatcctcta cctcctgaag ggcgtggttg atgtccggcc ttacccctgc tgcgccgctg
     6061 gcagtgttct gctctctgcc atgcaaactg ctgccagtca gtgcggggag caatatgggc
     6121 agccagccac catacatccg cagcctctgc ccagagttcc ctgacctcca gccggacagc
     6181 acggtttccg acacacctat aacgccagac tagactgctt cgtgaacggt ttctgaatcc
     6241 attcatttcc cctctcctct ttaattccgc ctgctaaata tgttaaaaaa acaaccgtaa
     6301 tccaacttcc agcgtgcatg ggagttgttt catcagacaa gccttattct tccgcctccc
     6361 ttcatctctg agggaggcat ttttttgctg ttcagtaata ctaaagcacc agaccactgt
     6421 atcggagtca ggaacggtca gagaactctg accagaaagc caataaaaaa cagatgaatg
     6481 gcatacgctg gcgcaaacca gcgtcctgcc tgcagacgtc gtccggaagc cggtaccagc
     6541 ccgtggacac agaacagcac cgtcagcgtc agaatcacgc cggaaaacat catcacatca
     6601 ccgtgtcggg cattaagcag ggcaaccagc aacagccaga cagcaaacag ggcgggacgc
     6661 agcgtgtccc tgacctgcca gaccagcagc gctccagcaa gcatcagcag accggggatg
     6721 ccgtaactgg cagaactgaa tggtatatac cccatcagtg ccgtgaaggc cgcaacacca
     6781 ttacgggcag tagcagactg caggaaaaga gccacctgca tgacaacagc aaaggtgaac
     6841 aggatattca gttgccacca cgcaagaccg acatcccgga aggccagcca gtaacccggc
     6901 tgggccagaa gcgccatcag ccacaggcgg ttcaggctgt gctgacgtaa ggttttaccc
     6961 gccatgttgc agccacagac cagcacaaac agggggaaag cacagcgccc gaccatgttc
     7021 agtaagggca tatccagctg aaaggcagta gctatgtggt cccccaccat cgccacaaac
     7081 gccaccatcc ggatgacatc gcgctgcagc gcggagaaat tcaggaccgg cctgacaggt
     7141 tgcacagcaa cggatttccc ctgattcatg ctgcggcctc cggacgctgt gcgcaaaggt
     7201 gcgccctgac acgcgccgcc agttgctgtt cattatcgcc acgcaacagc gggatacccg
     7261 cctgtttcag cacctcttcc agcagcaggt cacgccgctg acgtttcggt tcctgatgag
     7321 accggtcgtc cagctcaacc gctgcaatga tgcgcccggc acggtccgtg ataaccacat
     7381 cgcagtgcca ctgtgacacc aggcgaaata actgccacca ttcacggctg ccggacttcc
     7441 ggtcgggcgc cactctgacg atatcggcca cacgaacctg cggacataac cgccactggc
     7501 gggtatctgt caccctgtcc agccggcgca gaaagcgctg ctcccgttcg gtcattaacc
     7561 ggccggcact gacaaatgat ggcctgctgc acaacaacag ctctgctgcg ccaggccgta
     7621 taccccttga ccgcaatgac tgcagaagtg gatgcgctgc acgaccggcc tgacggagca
     7681 ggagcacaaa cccgcccaga agaatgacaa taataatgaa tgcaataaac atacgttcct
     7741 cttcctgact cagtcgccac caagcgtctt ttccatctct atctcttttt ccggcatctt
     7801 caccacccac tcacgttcag tacgctgcat ctgttcacgt acctgctggc gggcaccacg
     7861 accaccggca accgcttccc ggacagcctc atccagttcc cggctttccc gttctgtacc
     7921 gtcacgcagg cggcgctcat catcggcaga taacaacagg tcacgtttgt cttcgtcttt
     7981 ttgtggtgcc cccgacaaca gttcacgccg ggcctgttcc gccagttcac gggcggcttt
     8041 ctcctgcacc tccgccagct tctgctgctc atccggaggc agcgtaatgg ggtcttccgc
     8101 ttttgccgcc gtttcggcgt ccgccacgtt acgcaatgcc cgactcaact cgtcaggctc
     8161 tgtgatacgc cccccggtca gccgcgcggc attgagcagg cgctcttcgc cgtgcaggcg
     8221 tatcaccacc ccactctccg ggttttcctg cgccagccgc agtgcggcgt ccgcactgtc
     8281 agctatcagt gtctgaccat tccggctggc ctgcagtccg gcaaactgcg catcttctcc
     8341 gccacgcagc cgggattctt cgctgagcac cacgcgcatt ccgtcatcct caatacgaat
     8401 ttccgtcagc agcgcccctg cctctttccc gtgacgggtc cagactggca gggccacata
     8461 cggtgacggg tactttttac cggcagcaat gaagcgggcc atcgtctcac cctccagacc
     8521 gttttccgcc agcacccggc ggcccagcgc cgttttatcc agccgcgagg cggtggccag
     8581 caggcggtta ccggtgtcgg actcgcggtc acttttctcg tgcagcaggt cgtgggcggt
     8641 tttgccgccg tcagactgcc ggacctgttg ctgccagtcc tcaaggttgt ccgtgtagac
     8701 ctggacatgc tcttttgcgc gtgacaacgt cacataggtg gattccaggg acgccattct
     8761 cttcctgccg ccttcggtgc cggtcagggc aatagcaaaa cgctcgctgg cgccctgtac
     8821 gccatagact gtcagggcgt aggcgaggtc aatatgacgg tcctccgtca tggactgcgg
     8881 gtcgacaatt ttctcctgct caccctgacg aaagcggata gccccctcct gcgtgaatcc
     8941 ggccacctcc cacagactgt tggccacata accccggtca gtatcggagc gggtaaaacg
     9001 cacccggtct ccctggctga gggtcagttc acgatgggtg aagagggaaa tatcctgagc
     9061 aatgttctgc tgcggtgaaa tcagaaccga ttcaccgtcg gtattacgca gggtgaccac
     9121 accgtcctgt gggtttacgt ctgctaccat ccagtagcgt tcattcatca tcgccaccga
     9181 cccggtgtac tccgcaaagg tttcagctgc acgcagggcg ttgtccggca cccggagcgg
     9241 ctccagcacg gtgaacgtac gttcttctgc gccggtatct cccttctcat gccgggcgac
     9301 atgaatagcc tcattgatgg catgccggtc agcgttgagc tctgccataa tcagcgtctg
     9361 ctgctgtgcc tccggggtac gccccatcca gtcatcccgg atggctgcaa tgatgtcggt
     9421 acgcgccggt gacaactgct ccggcttcaa agcctcctgt gctgcagtcc ggccctgctc
     9481 cggctccggc tctttctccg gcttacggat ttccataacg gacttttccg gaacccatgc
     9541 cccggcctga cgcgggacct gctgtgggct gacgtcatcc acctggcgaa gggatgtctc
     9601 cacctgcccg gcgataatgc tttcaattgc cgggcgcagc gccggggtct gacggacaat
     9661 ctcctgcatc accaccgtat cgatggcgct acgctgttgt accagacgga acggctgccc
     9721 ggtggagaca gcctgcaact gcgcggtgtc cccgctggag accattcgcc cgccaccggc
     9781 ggcaaccagc tggtagagct ccgccatatc acggttgccc accatcgagc tctcatcagt
     9841 cagaaacagg acgttgcgaa agtcaggagt ttccccggcc tgaatggcct ggcgggtttc
     9901 actcaggaaa ctggcgagcg ttcgtgcctc caccccggcc tcacgcatct catgcacagc
     9961 ccggtgcgtg ggtcccaggc cgatgacctg cgggcgcacg gattcagaca gggtgttcag
    10021 ggcacccatc accgcccgga actgggtggt tttccccacc ccggcgtagc cctgaatggc
    10081 cataaagcgg tcggtgtttt ccagcactgt ccgcgtggcc tcccgctggc cggccgtcaa
    10141 accggccagt acagacgcag gcgtcagcgc catcaggggc tgaacggcat ccttaccctc
    10201 ggcaatatgg cggatgatgc tcttttccat ctcatacgcc acacgcggaa caagcaccct
    10261 gtttcccacc ctggaaaccg aatccagctg aatcagctcc ccgctgttca cctgccgggc
    10321 gatttctgtc cggacatcag ccagcggctg gccagatttt accgcttctg acagcagggc
    10381 aatttcgctg agatacacct gtccctgctg tgcgcgcggg atggccagat tcaccgcctg
    10441 ctctgcatcc gacatcagcc gctcccggct ggcctgtatg gcggtatcca gatttgtctc
    10501 gccagtcgac tggcggacct ggtcactggc aagccggaca gaagacacat tctccacttt
    10561 acgcgcggcc tgcccggcct ccagtggcgt ataaatccgg atatcaccgc cactctgcgc
    10621 cagcgcgttc aggacctgtt tattcagact tcgtggcccg accgcggcca gcactgtccg
    10681 ggaagcactc gttccggcac cggtcccctc cacataatcg tgggccagct tcacggcccg
    10741 ctcagtgggc agacgcagct cccggtcgcc actgcgcaat cgtatcgcac cgttttccag
    10801 agcagtcacc gtgaactgct ccttcgcttt gatggccccc ttcagctcac gccccagcgc
    10861 cctgacgcgg tccccctctg aaactgtcag cgtccggctg cggtacaggc tccagctgcc
    10921 gtcaagctgc gagaccttca gcgtcaggcg actgccgtct tctccctgca acaccaggct
    10981 attggtggct tcggccaccc ggtcaatggt gtgccgtgtc atcgttttct tctcagcgtc
    11041 ccaatgctcc atcaccatcc cggcccggta gttatcacgc tgatggcggg ttttgctgtc
    11101 cagccacaca ggctccagta cctgcagtgt catctgctcc cggcccagtt ctccggcctc
    11161 acgccgtgac tcgcgaatga tatccgtcag ttgctgctgc tcccgggcac cggtcacctg
    11221 ggccaccaca tcccgccctt ccgccgacag gcgcacatag tcctgtgcca gctggctgta
    11281 acggcttcgc ttgtccgcct cgctgaccag tcgtacctcc ggtagttggg tgccataaaa
    11341 ccggtactgc gggataccgg cttctttcag cacagacagg gcattccccg ttccctgccg
    11401 gttctccgta tccataaaaa tcagctgcgc cccggcgctg agcgcctttt cctgcagcag
    11461 caccgtctct ttcagcgaca gccgctccgc ccggtcaacg ataactgtcc cctgcaccgg
    11521 caatacggtg tctgcattca tctgcgagcg cagcattaca tggcctgcca gatgcggact
    11581 ttctgacata aactggccac tgcgaccgtc ggctgcaatc accatcacct cacggccctg
    11641 tgaacgggcc atcatggcca cgtcttccgt gcgctcacgc agggcctgcg cgcctccgcg
    11701 accactgaga atggccaccg ggcttttgtc ctgcgtcaga acagagacag catcacctgc
    11761 cggtatatcg cgttcctgtg cccgttcagg aaagagcagc acggtctgtt cctgaatggc
    11821 cgtgcgcgcc agctgatgga cgctgagttc attcagcagg tggatatcag aggtgaatat
    11881 ccccttctca cggtccagag ggataagacg ctgcccttca atcgcggcat cgatgccggc
    11941 ccgggcctgc tcaaacagac cgggtgcgga aggcagacgg ttgaccgtac cggccagcat
    12001 ctccgaccag gtgaactgga cctttttctc cgacaacgcc gaaatcgtgt cgctgacagc
    12061 tttccgcacc tgctcgtcag caatctcgcc ggaagacgcg cctcccgaca ggtccggtgc
    12121 atgtataccg ctatgtccac ctgcaacagc accaggaacc tccacgcgcg cctgcgcctg
    12181 tctgatgtag ccatcgatat caaatttttc atcggccagc cgccggcgcc agtcagcttt
    12241 cagcagctcc gggtcggccc aggctttggc ctgacgggta tcgagcgcgg caacgtcgcg
    12301 ggatttggca gacgcgtccg ggccggcggc ctcccggata gtctgactac gtgaggaaaa
    12361 aacatcgaca gggacatcct tcagctccca cagaccattc tttccggccg caacagtttc
    12421 aaatcccatc gactcaacgt cttcacgtaa agcagaacga tacaggtttc ccagcgctat
    12481 cttggtggca tacagattct cggagaaacc cgttttcatg cgcgtatcac tggccagcga
    12541 ccgccatttt tcaccggcaa acgtggcgtt aaagaccaga gcgtgggtat gcaactgcgg
    12601 atcgaggtca cgggacgtat catggttata gagtgcggcc accatgttgc cggtcagtac
    12661 tgtttccgtt ttcccctctt ctgtaatgcg cgcgctgacc agcttctcaa cctcctgcat
    12721 caccaccgct acagcccggt tatgcgcctc aataaagcgt cggtcttccc cgaccagcgc
    12781 catgacggag acacttttgg gggcagaaaa cgtcaggtcg tagccgctgc ggtgcttatt
    12841 cacaccattt accatacggg agagatctga accgtccgga agtttaccct gacgcacagc
    12901 atccatatcg gcgctggcga cttccccctt cagtcccagt ttctccgcac cctcccccat
    12961 ccagcgggag tccagtgaac cgctggcgta gtagttgtct tcatgactgt agtagcccgc
    13021 atcaccttta atggaagaaa tggagagcat cagaacacct ccctgtcgtc atcaaaatca
    13081 cgcgctttat ctgtcgcatg attgatgttg acctcttccc tgcgggtcat atcgcgggtc
    13141 tgatgtgaag cctgaaccca gtcctcatac gcttcggaat ccaccacctc accatcgctg
    13201 ttcagacccg gaggaacgtc cttaggtaaa tcctgttcaa tatcccgctc agttcctccg
    13261 gtggaggtgg agccaccttc cgctgcaaca gcagtggatc ccgtgttttt ggtatctgca
    13321 tcgccggcag cgttttctgt ctgtaatgtg cgcgtctcag gaggtgtgtt cgtttccgca
    13381 agaacagcag ccacccggcc ggcagaagga gggggcgtat tcgacagatt actctccgga
    13441 acagcctgaa ttaacgacgc cccttcgaac aggccggcaa acttatgcct ctcttcttca
    13501 gtgcgacggg taatttcatc ttcaagcatc tgatccagac tggtctgtac atcacgcaac
    13561 aacagcgagt cagccacttt gggcatcgcc tcatacttca ttgtcagttt caccaccgga
    13621 taacttcccg gcagggtgac aaaacactgc aggtcgttca gggactgcac atcggagtag
    13681 ctgacaatac tgacgcgttc ttcttccttg ccaaaagaaa tcccgtcacg cacctgttca
    13741 tgaccaaagc tggtctgttc actgaatttt tttcgccagg tttcaccgat atcttcctcc
    13801 acaaacttcg caatctgggc gctcggggag cggaagaaga atttggtatt cagcaggtca
    13861 aacagcgccg ccgccgcttt cgggccgtaa atttcttcca gctgtgcgaa gctctggaag
    13921 cccaggacaa aacagccgcc aaacttacgc gcctccgaaa taattcgtgg cagagtcggt
    13981 aatttgtgca aagaagccag ttcgtcataa aaaaaccaga cacgtcgctg gcggttcggc
    14041 cccatcgcca gcagactggt ggctgcaata cttagccaca gtgaaatcaa tggcttaaga
    14101 gactcatggt tctgttcgtc ggaggtaata aagagccagc catttttcga tttatcggcc
    14161 tggcccttca tccattcccg gatggtaaac ttttcgcgtc cgggcctgtc gataccctgc
    14221 agataacgca tcgctttgac gtagttagtc agcacgctgc ggatggaaat ggcggtcttc
    14281 tcaatcttac cgtcaaccag ggtggaagcg ggcgtgccgg caagaaacgt tctcagttga
    14341 tcaagctgaa ttgccagcag agtgcgcagg aatttattgt agctgcggtc ttcatccctg
    14401 cgcatgcgtt ctgcgccttc cgcaaagatg gtacgcgcac tgccctgcca gaacggatct
    14461 tcaccactgc tggccgggat caaggttgca ctggcattct ccagttcgga aatcgtacgg
    14521 cactcttccc acaaatccca gttggggcaa cgggcatcca gcgcattaag cactttatcc
    14581 cgggattcat cgtagaaatc ctcaatgaac gtacagccct tgtcgtagat aatgaccagg
    14641 tcccccctgt cgcgcagttg cttcataatc ttgcgcatca gagtggactt accggtcgat
    14701 acagtaccgt gcatggacat attctgtatc tcagaatcca gcttcagcgg caggtcatcg
    14761 atgcggatat cggatgcttc cccacgtttt ttcatcatcc gggcaacgtc ttttggtgac
    14821 tcactgagag cgcggccccc gatgatttca tcctcactct gctggcgacc ggtgcgcccg
    14881 atataccagt acacggcgaa tgtggccaca aaggcgacca gcagcgcggc gcaaccagca
    14941 ataatcagct cctgcttcag caactgaccg caatatacgg tgtaatcatc attcagtatc
    15001 tgacgtgcac tgtactgcag cttatgttcg taataacgga tggtatagac aggctgactc
    15061 accatttttt ccgtaaaagg tgaaatcatc cgaacaaacc agtaaataat accgttggtc
    15121 agattctgga tgcttattcg catcagcaga acggcaatac caaataacac cgtgcccatg
    15181 ataacgtaat aagaaatcag attatttatt tgcaggaaca ttttcaggcg cataaaagcg
    15241 acctgaccgc cctgcgttaa atcgcgagga ttcaggctca taatattttc cggagaataa
    15301 tacttagtga agatacttca gaaagaaacc ttcacgaaca gaacgcgtta attctgattg
    15361 cgtcagagaa taaaataaca gggaaacgtt ttaatacggg tggccactgg ccacccggaa
    15421 agtcttacag gatattagca actgatttcg ccagttgatc ttcaagaacg ggtttggctt
    15481 cttcaaattt gagattaact ttattagcgg aagagacaat acgcgtctgg tatttatgct
    15541 gattgcctgt ctgcgtactg gtctgaacct tatatccgga tgttccttgt ttcagcgcag
    15601 ctacattatc ggtctggaca gtggcattcg ttttttcaga aatctgaata tctgtgacca
    15661 tggtgtagtt aatatcttca accatcgcat cagcggccat acccacgaga ccggcagcaa
    15721 gacccacacc taatgtcgcc cccgcagagt tggagttata ggccgtaata cctgccccta
    15781 acgcagctcc cattgctgcc ccctgatacc cctgactcag gaatccctgg gctgtgcgca
    15841 gatccatttt atcggctttg aggacattgg cctgtatcca gtaatacgcg ctgtccggcg
    15901 cggatgtcac agtataccct ttatcctgaa gcgccttagt cactttcgcc tgcagaccgg
    15961 acatgtcttt gtcggaggta ttctttatct gaagatatac ggttttctgc gaggacggtt
    16021 caagccagat ggtttcactc atctgggttt tcacttcaag attacgtttc ttgattgctg
    16081 tactcatcgc tccacaacca gacaagacca gagtggaaga caatatggcg gtcgctgcca
    16141 ttaacttaat gttttgtttc atattttatc atttccgtat aagtggcatg atatctttca
    16201 tgctcagaag tgctgctatc attcctccct tctcaggagg taatggctat cttataacca
    16261 gaaatatacc cgcatcaata tcacagccag agttggttgt tttttaggct ctcatagtaa
    16321 aagtccgatg aataacttat ttcggggatg ccgaaataag ctctttaatt ttcgataaac
    16381 tactgaccac tgctggggtc atatatctgg ataacgacat ttgaaacgac atcatgcaga
    16441 tgagatatgt aacaaaccat gaaaaaccaa gccccgaaat aaccccttca ccagagagta
    16501 gtaaacctac acctataatc aggtttataa ccacaagaaa tttccagtac tgtttaagct
    16561 ttctcattaa caattttaca attagggaat cacctttatc cacctcatta atcgacaaca
    16621 ttatgctgac aggttgatat aaagaaggga caaacacggc tgagaaaaac aaaattccac
    16681 ctgcagacaa aatgcttata ccaattaatg cctcatgatc gtatctgtag atgatgaagt
    16741 gacataaaat cattaccaac tgaataatta ttaccacttg tattcttttt aatataattt
    16801 tcattacatc agagcttttt ggtatttcat aatcactttc ttttattttc tcaatcaaag
    16861 agctaacatc ctgctctaac actgatttcc tcatactctc accttacgtc tttccattaa
    16921 atctattact catgtcattt ttgatttgtt taatattttc atcatgacta attacaaagt
    16981 catttttata gaatttctgt ctttcatttt ctgccttttg agcgtcatcg aatgctttct
    17041 taccctggtc atgttcattc tgcaagtctc tgtattcctt accggaagat tctccagatg
    17101 agatcacagt atttttttca tcattgacct gtgattcaac attactgcgt tgattctgga
    17161 catccgtagc caccacaccc tctgctttta cgccgtgctg cgcagcgcta ttttccatgt
    17221 cagcctgctg ttcacggtat tctcccttca ggtcagaagc cactgttggt gccgatacgt
    17281 tcccaatccc gtctccggta cgacctttgt tctgctcaaa ttgctgtaac aactgcggct
    17341 ttatgcgctc ttccacaaaa gcctgcacca gtctttcgcg ttctgcccgc acttccgggc
    17401 tggcagtgtc agaaagcagc tggtcagcct ctcccggacg ctggctggtg acatacccga
    17461 caaactcctg cgagtagttc tggttaatgt tcgcactgtt gttctctgca tagctggcca
    17521 gccgggcata ctcatggcta cgggtaacgg catcattata ttgtgacgcc tgactcttca
    17581 ggctggagaa ggtggacgac agctggctgg ccagggaagc agactcattg tctgtatgac
    17641 taccactgtc agtgacacgg ctggaggcca ccacatccat tgcatcgcga aaatctttaa
    17701 gctcctgagc actgaaccct ttactgctcc cgccctggcg ggtcagatcg gaactggtcc
    17761 cgtgcgaaga tccacttcgg ccagcgtatt tgacatgccc ttcagcagag cctttaagtc
    17821 ctgttgccag cccaccaatt ttaccgagta aatggcggtc tgtatcaaat gaaacctgaa
    17881 ggcctccacc tgcgctcgtc tcctgacttt ttgtcgcggc ttctctcacg gcttcagcca
    17941 gactgacatt ttcatcgcgg gcatagcggt taccaatcga ctgaatggta ttcagcgctt
    18001 tggtcagatt actgctgttc gagatgtcac tgcccaccac ggtggtgtcg ctgttacccc
    18061 gctgctgact ccactggctc atctgcgacg cggccaggct ggatgtctgc gacaggctgt
    18121 tagtgaggct ttgcgcttcg ctcatggcat cacgctgttg ctgctgccag ctgctcgttg
    18181 cggttttgcc tagcgcaata tcgaccggca gttttgaaat cgcttctgtg gtgttgtaaa
    18241 ccgactgtcc ggagctcgtc agcgtcttca tcgccccgct ctccagctga ctggtcatat
    18301 ggccttcacg atggctgtag ttggtgtccc acttgttacc ctgtacgttc ccggttgtca
    18361 tattgttgta gtgccagtta ccgtcaatgg tctgagacga cgattgcgtg gaggcagact
    18421 gcagggtctg ccccagatag ttccccgcct gcgataccac ctgccccagc ccttttacca
    18481 tcccgtaagc gatgaacggg atggacagcg ccagccagcc ggcggttgtg ccaatatccg
    18541 agtaggtatt ctgtacctgg tcaaaattag ccagcgttac tgccgtacca ctgagcttac
    18601 cctgcagata gaagttcatg gcatggttca ggatggagaa caggatgggc cacatctgca
    18661 gataggcgat agtatagaca taccctttaa taatggccca ggacagggaa ctcaccaccg
    18721 caagcacaat cagtacaggg aaaagtgcta ttgtcatccc caacagtacg ctgtacatca
    18781 ccggtagtgt gttagccgca atcgacgcac tggtcgcctg tgacagtcgc atttttgcca
    18841 ttgaggtctg cgtggctaca ttcaccagcc ctgccgtatc cccgctcagt gcggaaaaac
    18901 tctccagtcc gctgcgcagg ccgttcatta tcacattctt tcggatgatc tccgtggatg
    18961 tcagggaagt acgatgaaaa tagttatagc tgtcacccag catttcagta aacagcgccg
    19021 attgcgcccc gttcgtctga ccaaatacct ggtcagtgaa gccgtgcagc gtattcagga
    19081 tctgttgaag aaaaggtgac gtattcgccc cggaaatatc accggagtcc gtattaattc
    19141 tggtggcagc ctgttcacag gtcaggaaat tacgattttt gtcgtagagc ccacgcagcg
    19201 gactcggacg gctgaatatc agtgcatagg ggtcgtccgc attcatcagc tccccgatgg
    19261 tatacttgtt gttcagcatg atgtcaccca ccacgcagtt atgaacataa tccgagaaca
    19321 tgcgctgaat ctccggctca gagaaccgga aatcactgct ccctgcagcc agctgcgcac
    19381 caaaaagcat cccggtttta ctgtaggtca gcgcgtcagg gcgggcaaaa atggtatcgt
    19441 acgtctgtgc cataccggca ccgatccggg taatcatact ggcgggcgca gccaggcctg
    19501 caggaacatt atccacctga taaatccggg ccggttcaga caggtcaata atctgaacgc
    19561 ttcgtttatc gccaatcaaa acggcaatga ccagaacata aaacccggcc cacttgacca
    19621 gatccataat attacgggtc cggatataag ttgcagcgcc gacaatgact gacacggcgg
    19681 tggccatcgc aatcagcgca tcgcccgcac ggctgtcgag aaacgatgcc acgccattga
    19741 gcgtatcctg aaaccacgca ccaccggcaa tcgtgtaaat ggtatcaatg gccatattaa
    19801 ttcactctgc tgaactggta gttacccagc acgcgatcgc tcatctttga ggagagctgc
    19861 tggcggataa agcggatttg ctgttgtgcg gaaataagcg cattttcctt aatctgaacc
    19921 tccattcgca gtgagttgag agcctggcgt gcatctgcca ggccattttt cagttcattc
    19981 atattggcac ccggatagtc tttggtggcc agtgagccat taaccacctt catgacggcc
    20041 tccagatact gcaggtaaat gtcgacggcg atgtaatccg tcagctgggc aaacagggac
    20101 tgcgagacgc tcagtgatga ctggtccact atccatgaca ggatggggat ggaggtgctg
    20161 ttgatgaact gcttctcttt atccgtcaac ggtgtgtccg tgatggcttt attttctatg
    20221 cttgtcagcg tgtcctctac catgcggatc aacgactttg actccgaaat ggtcacgtta
    20281 ttcagggagg gtgacagaca cttgttctgg ttatcacaga cgtacacttt ggcgctgcca
    20341 ccactcatca tcgcactcag cagactgcgg tctgacgcca gtgaagacag gatacgcatt
    20401 tctccgttac tgccgaaaat caccgaaccg ctgatgctca tggcaaactg gcgcagctct
    20461 gcattgctgc tgagcgtact gttgctgaga gcatcccaca tcaggttaat atccttcagg
    20521 acctggtctt tttctgcgcc ggtggccttg ttcgtgacgc tttcgtattt cccgccggat
    20581 gtgcaaccct gacgggagga gacccagtcc gagaacacgt tattctgacc ggcgacagac
    20641 tggcagacgt tctgctgact ttccccccac agtgacgcca cgctgtcgac catcgctttt
    20701 gccgcctggc agctggacat attgaaacgg ttaaggtcag cagccatctt ctgcaggaag
    20761 tcttttgccg ctttcagctc aggcacggta gtttccagtg caaggtcgaa aaagtacccc
    20821 gccgcgttgg ccatcgtctg tttcacaaac gccataatct ggtcggagtt aataaaggag
    20881 aaagatccaa gatagacatc aatcccgcca catccggcgt tgatggacgg caggctgatg
    20941 gatatcagct ggatattttt gtttcctgtg cgggcataga acgaaccgcc ggttacatag
    21001 cgtgccgcct gtccctgcca ggctttcgcc gaggtggagt tactggcata actcatattg
    21061 ttgaaaaacg aattcatgtc atcctgcagc ccggcctgtg ccgttcctgc actatacagg
    21121 cccagcgtaa acgccaggca aagcggtttc agtcgcatta ttgttattcc tgagaatgag
    21181 cctgaaggga tttcccggcc tgacggtcca gggtcatacg gaacacgtca tcgagacgtt
    21241 tacggacctc tccggcctct gcagcaccct gcagcagagg ccaggtttcc atcgtgttaa
    21301 cgttggtcag aaaagtggtt ggggtggcta ccggcacgcc actctggaaa aactccacca
    21361 tcacatccgg tgtggccgga agtacatcgg gaaattcgac atcacctttt ccgtcaaggc
    21421 ttacagggaa gacactgaga ccactctgct gcgacagtga cttcagtacc ggcgcaaact
    21481 ggcgacagta ttgacaggtc gactgcataa acagcaccag tttccagtcc tgcaaatcca
    21541 cccgccgccc gtcactcagc ggataccaga cgggcgacgt cttttcgctg agtcctggtg
    21601 acggtggtgt gttgctctca ggaagacgca atccatccag tgcaggtgcg ctatgtccgt
    21661 ttttcccagc ttccagtgca cgaatatcat ccagtgtccc tgcctgcaga cagccggaca
    21721 gcatcgccgc catcagaatg gcggttcgca atacgagaat aagcatgaat tacctcagaa
    21781 caggcgttat cagtagtcag gtttaaaatg ggtaagaacg ttgaccatct ggcggtccag
    21841 gtcatcgtgg ctcataaatc cccatgcaag aggctgccac tggtgcgttt tcggatcgac
    21901 caggaaggtc gcggggaaat gtgtggcgcg catctgctca gcctgcccgc tgtccggacg
    21961 gctctgcggc agctggtcgc tgattttacc gtccacactc acggccataa gtgtaatgcc
    22021 ccggtcttcg cagaacgcgc ggatgacacc ggccatcagg ttgtcgacgg cattattccc
    22081 ccggtagaag aagaacagac catagcgctg tgccacctga cggatgaccg cattctgaac
    22141 ctttctctct tcggtaagac gcgcttccac agaaccgttg tagtgactac gtttcacgtt
    22201 gtagtcgagt tccggatatt tcaggcgcgc cagctttgat gtctgggtaa aatctgtcgc
    22261 ccggtcagtc cagaaattct ggagtatcat aaaacggcgg aagttctccg cagacggata
    22321 cagaatggcc gtatccagcg cttcacgggt cgcctgctgc aggacctttt tctgctcaga
    22381 tggcgtcatg ctggccagac gttcctgaag ggtcggctct ggcgcaggct ccggttgaat
    22441 ctcttccgca ttcgggttgt accactgcca gccttcttcc cgggcatagt tggggaccag
    22501 tacatcgtcg ggagccgcat gcagtgatgc agtggcaaga agaacaagca caccggcgac
    22561 agctctcatg gcgcgttctc cttcatcttt tcagcaataa tgtcctgtgc tttcttcagc
    22621 aaggcctggt cttccgggat atcgctgccg gcattaagat catcatagaa atcgctgaaa
    22681 ttcatcacac caaagtccag cttctgcagt tcatccaccg tgatgccccg acagtccgga
    22741 gatttgccac tgccgaaacc aatgcccagc tgtccacggc gtccctgctc ctggacaatg
    22801 cgtgccagtt tggaatcaaa tacgcagtaa ccgcgtttct tctccagaca tacccccagc
    22861 acttttttgc tgcagtattc ccctacatag accgtcagtt ttttctcttt agccttaccc
    22921 agtgcctttt catcactgct gcaactggac agcccgatat cctggcccca gccagaatcc
    22981 ttacagcaat tgttaaaccc ggcagcaaac ttcttgcagg atttggcttc gccagtgaat
    23041 gccctgacat tctcgccgtt cagttccgcc acatcctctc cggcagcagc cactgccgcc
    23101 agcgctgaca ctgcctgacc aaacatattg ctggtccctg tctgcgcctg ggcacaactg
    23161 ccgtctttgc agaagaactc cccgccgcaa atctggccgt ttccctcttt tttacgctca
    23221 caggagtagg tgacctgctg gctgacacaa aaaccatcca ccgtatcggc acactgactt
    23281 cgtacaaccg tacaggcagg gttcttcata tactcaccgc aggttccctc agtttcggtc
    23341 tgtgtcagat atgtgtcctg ccacttccag cagtcctggt ggatgctgta ggtttttccg
    23401 tcaacaacga cggtccgggt gcctcctggc tctacacact gagatccggt cattgcacct
    23461 tcagacttgc tgaacgggca gctttctgtc cagacaacct caggtaccca ttttctggaa
    23521 ggaacctgaa tcgtcagcgt aatggtgaaa cggtacttca tatttgagct ataccagatg
    23581 gaagacactg taggtttgtt gcgtctattc gttatggaaa acggcaatga ctgcccatct
    23641 gttatattca gcccggaact cagcgtgacg ctcccctcag aatcatccat tgaaattaaa
    23701 ccaaatggag tggtcactct catgaaccag tgttcgttgc cgtaagcaag tttcttattc
    23761 caggagtaaa ccgcgctggc agcgatcact ttccctccag tgctgacctt aaattcacca
    23821 gaaaaagaat tgttcttttc agtgaaccga atcgaggaag attcaatcac tatctgtttg
    23881 atttctgtcg tatcctcata gtacccactg atggtcgctg tacgggtaca gtactgttca
    23941 attttcgtgt cccgcaggca ctggtgtgtg gtgatttccg tgaagccagc aacctgatcc
    24001 acacaaccat caaatccacc accggtaatg gcctccgcct tatccttcat ggccagccct
    24061 tctgaaataa acggcgcatc cagcgacggc ttgttatccg gcggcgtatt gagaatggac
    24121 tccgtgatta ttttacccgc ttcagatgtg ttcagcgctg ctgaacccgg ggcggtcata
    24181 tcgacgccag gggaggtcac gccaccgtaa tacccgcttt caggagggga gttggtataa
    24241 cccgggatga cctccgcagg attaaacccc tgaatagcac tggccccctg tcccttattc
    24301 tggctactca gattcattcc ctggttaaac tgctcatttg caacaagcgg ctgtaccagg
    24361 cagaaagatg ccgccagtat cagtaaagga aggtatcgtt tcattccccc ccctcgccaa
    24421 gcaggctcct ggcggtgtca gcacagtcgc cctcttctgc cacccgcttc agggcctgat
    24481 gcagcgtaag atccccctgc agacgatcga ctttctcacc ctgattacca caggctacaa
    24541 ccagcaccgg cacggcggta atgttgtatt tacgaaacgt cgtcggatct atctgtaccc
    24601 caccggtatt gctctctttc acaagaccgg caacggcatt agccgtcgcc cgcatatcgc
    24661 cgttaaccat tccccggata accaccggaa tattgagttg tgccgcatcc tttacacgct
    24721 gcttcaactc ctccgccggc atcgagaagg acacaaacac cagggccgca ttcaccgggc
    24781 gttccccggg ctgcatggat tgccggaact gctgtgcctg cgcgtcgaga aacgactgct
    24841 cctgcggtgc caccggacgg gtctgtttca ggaattccgg ggtttgcaga gactgaaatc
    24901 gctgacggtc cagttgcagc tgctcatgga taaactgacg gttctccgta tgattattcg
    24961 ccatgccgcc cccgctgacc agaagcaggg ccagcgatac cattactcgt ttcatcgcgt
    25021 ttcctcctgt gacgggcggt gtgccatacc gtaggcgcga tccgcagaac gaatctccag
    25081 catctcctcc cacatgcgtc gttcccgtac tgacatctgc cgtctcagta ccggaagcag
    25141 accgcttatc catctgtcca caaacgccag acgctccagt gtcagccata cctgttcact
    25201 acctgacgat gacagcgcta tcgccgcttc ccggcactcc agaatacatt tgaacggccc
    25261 gccatttttc ttaagcgcgc cgttcagctc tgcctgctga cgctccagca ggtcagcacg
    25321 ggaggcggcc tgttcgtagc gggcgagcag aaagtcatac ttttttttac tcacgagcat
    25381 actgtgctcc ttacagaaag acgcagttgc gcttacgcca gaagagatac ccaaagttac
    25441 gccggtcatt gggcatgttc ttgcccgctt cccagcgggt tacgctacgc cccagcggat
    25501 gacactgtcc ggcatcggga tacttgttga ccatgtgata tcgccagcgg cttttgggga
    25561 tgatcgggga tggatattca aagcagacag ccctgttttc accgatgctg ttcatgatca
    25621 tgccctggcg gtgaagcttg aacgccatgc gctcactgag taacagtgac gtctgtaacg
    25681 gactgaattc gttagaaaca tagccgttaa acgggtacat actgccctga ctgccggcac
    25741 accagaaaag ggcgtcgatg ggtttactca ccagactggc cacggcatca gcggcacagg
    25801 ctccctgggc gatgatgttg ccaaagagaa tcgcttccgg ctggataatc atgctgagag
    25861 agctgctgtc ccacatggga tcaagttccg acagataacc aatatccagg tcgccaccct
    25921 gcaggcagcc catcgacgtg ataatgttca gccagtaggt caacgggtat ttgtaccagt
    25981 gaacatggta gaaggccccc ggtttggcgc ggctttcctg accaccactg ccgctaccca
    26041 cattaccaat attgatatta aagcccccca gattcaccat gcagtaaggg gagcgtgtca
    26101 catccgtcag cgccatcggc tcccagtaac caatggccag ccccacccgg tataaaatgc
    26161 ccatcgggca aaactggatc ggcatggaag gattcggcgt atccggctga cgtcctttgg
    26221 cgaccggaac attgccaatg gttaccggga aaatacactc ccagcagata tccgttattg
    26281 gattgacaaa attgccttca cattcagccg aagcggccct ggccgtcccg gtcaggcata
    26341 acaacatcag cgccagtcct gttacataac ggttcatggc ttcacctccg gcacatccag
    26401 tgcaaacgaa tccacccgca gacggcgacg atcttcagcc agcgtcacac gggctgggat
    26461 ggttttgagt tcaaacttcc ggctgagcga gccatcctga tcgaagtaaa tgcgtgtatt
    26521 cagggcattg gtcgcttctc gaacatcccc attgaccagt atgacgcggt acgtcagcgt
    26581 gccgggattc tgcttcttca tccacgccag ctgctgcgga ttatccgcat caataaaata
    26641 cagcgtgtcg gtaaagggca ccgtatcaag cggattaatc acctgtccct tacgggcaaa
    26701 cacccgcccc tgatggtctt tcagatcttc actgaccgtc agcgatggat cgatatagtg
    26761 cgtggtgttt tcctgcgcca gcgtcagccc ttccaccggc ttaggacgca gggtgttttc
    26821 tatgacacgc tgtttgaatg cttcctgctc ccgggccatc tcaccactgg cctccattgc
    26881 tttcagccgg gtctgaatgg tggtcagcat gtcctgctcg gcaatggggt acatttcccc
    26941 ccatgtgccc aggttttttg cctgtaccat gccggacagg gcaagcagta tgagcagacg
    27001 tcggtacatt actgcccccc tccggcggcc attttttgtg ccactgcaga ctgaatttca
    27061 ccggtaatgt ctgccgcacc gctgaccacc gccggcgtca ccaggataag gacgttgtga
    27121 tcgcgctggt attcctgaag ttgtgtgcta agtgtctgca taaagcgttc tgtcaggacg
    27181 ctcatctgcg cctcattcag ggactgtgcc cccgcctggt cggtgaactg atcgaccgtg
    27241 cccttcatat caaaagacac aaccgtcggt gtacgccagc taatcagcag agacgtgacc
    27301 gccgcgttaa cgcacaaaac agtggcggcg accagcagca gattacgcag acagcggcga
    27361 cggcgttgct cccgccggct ccctttaaca ttaatattcc ccccttttct gactgactca
    27421 gcagaaacag aggattcaga cggtgctgag ttaaggtttt gctctgtggt catgctgcct
    27481 ccgcaagcgc ttccagttcg cgcatctcat cagggaagtt gcgttgtgcc agcaggtaga
    27541 cggcgtcatg gatatccatg ttctgttccc ggcactggcg gataaattcg aagtcatcgc
    27601 cctttgaact gaacatcgcc cggctgagcg ggtcgacgaa gagacgatgg aaactgcagg
    27661 tatcgttgat gcgcagcata aaggaagaga accactggtc tttggcatcc ccgaatttgg
    27721 aaataacgct gcgttccagc tcgctgaact ggttggggcg gttctggttg tactgtttga
    27781 attccgacgc atcctgcttg agaaccacct tgaaggcaga gttcccccag gctgcttttg
    27841 ccgcgccaga ggcatcatcg gcatcaaagt ccttgatgtt ctggctgatg gtgatgaacg
    27901 cgccacgatg acgtcgcacg gtacggtagc cggtttcaat aaactcaccg accttctcat
    27961 ttttgaagtt gagcagcttc cagccttcgt cgatggtgca gcacttcttc aggctgcgcg
    28021 gcgtgcggta catcttgtcc tcaatgtaga tgatgaggga aaacattacc gcaaccagca
    28081 gatcaggctt gtcctgcaga ccgcccagct ccaggacaat aaagcgggca tcgtcggtca
    28141 gtgacggctc atcgctgttg aaatattcac cgtaaattcc ccaggagcag tatttcccga
    28201 gtagctcgat aatttcatcc agtcggttgg tgatgcccgg cgaattctga tatttatcat
    28261 gctcacggat agccgtcagc ttatggacga catggtcaat acgggcggtt tcctcgtgtt
    28321 cttcccaggc ctcctgaacc ccgcgcaaca gcaggctgtg gtggacttca tccagcgtac
    28381 cgttagggct tgccagcaca gccagctggt cacggatacg ttccccggac tcattgatgt
    28441 tagagatgtt ggcaaacgga ttaaatttga gtgacttacc gtcaaggtag gtgccaccca
    28501 cgttctcaca gaacgatttg tagccgtccc ccatgtcaaa cacccaggcg ataccaccgg
    28561 agtccagcac gctgcgcagg ataggctgca ccaggccggt cttgcctgcc cccgaggtac
    28621 cggtgaccgc catgttgtag ttggtgttgc ccatcccgtc cccgtagata tcgaggaagg
    28681 ccagctggtt acggtaggac ggtgtcagca gtcccccctg acacagccgg ttgtcggcca
    28741 ccaccggcag cagattcacg gcctggatac tctccgcccg gcacgtggct ccgcccattt
    28801 taatgtcctc ccagagccct tccgcatgaa tgaaggggat catcgctatc cagttgcgga
    28861 actgcatata ggtcggggaa aacagctcta taccgttttt cttgaacgtg ttgataacct
    28921 gctgttcgca gaccagcgca tcctcatccg tatccggaca gaatgtggtg atgttatagt
    28981 aataacgaac aatacaggtc tggttgctgt taagacgctc gcggatttcc ttccactcct
    29041 gggcctgttt ggccacaccg ggaaacaggg tggcataggg ggatttcgct tttttgtccc
    29101 ggtcgagata ctttcgtgtg gcctcccctt gcgttgccac ctggtcctcg gcctccatta
    29161 ccatggtgat gacaaacggg ctggcaatgg ccaggtccgg gctgagcaga ttggataggt
    29221 tatcgccgcc catccacagc atgaacatat ccgggttatt ttccagcata aagttcatgg
    29281 cccgtgttgt ggtcacactg ccctgcctgt cagccaggct gatacgcaca tcgtcaggac
    29341 gcacatcgat accgatacgc tgatcaatac agcgctgatg cagttcatca aatttgccga
    29401 cacgtacgtc gtccggatac aactgatcat ggcgggcatt cagcatcccg tgaaccagcg
    29461 tggccagttc ctgagcatct gccgtctgtg tgaaaatttt tgccgcatcc agtgaagaac
    29521 gaagaacttt cagtgtgtgg gcaacctccg tcatgaccgc cgccgtgcgt ttctttgctt
    29581 tcacacagta ggagatatac aggcggtagt cccgcagggt cagcggcaac cccgtaggac
    29641 tgttgaactg ctgctgtgca gcacgccggt aaaacgcccg cgtgatacgg ttaaatttat
    29701 ccgcacgctt accctgccag cggaattcag acaggccacg atccagctgc tcgccaatca
    29761 gcttactgga caccaggtgg aaggaaatcg gtgtcttacg cggcagcttg cttttgatga
    29821 ggtcttccag cacatacact atctgttcgt tagcaccgat aaggggttcc gcccgcagca
    29881 gaaaaccaac cgtactgcgg ttcacaaaca gcccggagtc acggtcatag tcgcgatacg
    29941 gcaggacgct ggtcagctgc ggaaaatcca gactggccag attacgcgtc atctcagatg
    30001 caccatccgg catacggaaa gcattcgtga ggttctccag cacgctcaga aattttttca
    30061 ccagaacctc cttaatttat gcgggccgga aggacccagt cagcagggct gaccacaaaa
    30121 gagacacgac cgggctgatg gaaagcgtta tcgctgtcca cccagggcgc gatccagacg
    30181 gtggctatgc gatcccggct gcgctgagga tgtacagagc cgggattatc acaacgcacg
    30241 gtctggcatg agggagccgg attgcccacg gacacagccg gacgcgaaac tggtgttacc
    30301 ggcgttccgg caaccagacg gggcgtcagg gttggcgctg cggcattcgt gcttttacct
    30361 gttgcgccgt caactgatac tgcaggtttg ttgctggtta cctgtatacg ggaagaggtg
    30421 ctgacagcat agcgggatgc tgacggaagc accgccgcag aagtggccgg gagattaacc
    30481 agggaaggca gtccgcccgc agccggcttt cccgcctgtt ttgccgcctt gtcccgtgcc
    30541 agctggttgg ctttcgtcat ggtcatacag gtatcagacg ttgtggcatc acagtcaaag
    30601 ctgcttttaa cgccggcaca gccggtcaac agcaggacac tgcctgccag caaaaatgta
    30661 atttttttca tcgctgtttc ctgaaaaatt atgctctgaa gagtctggct ggctgcgaaa
    30721 gcgcagcgct ttatttatca tgatgcccgt cccctggctt cagcggatac cagacgttgc
    30781 agttctgacg gataacctcc gccagcctct ccccgtcctc accgctatac agtgctttaa
    30841 tctggctgac gtcagacttc gccaggaaaa accggtacat cggttgtttg gttatgctca
    30901 tacctgcccc cggagttccc gggaacaggc agtgccctcg ttcccgctgc agtacaagta
    30961 cgtcctgaga aatccgcaac ttcccctcaa ggacggtagc gcacggcaga gacgctgaga
    31021 gccagaagtc gctctctgcg tcatcaacac agatgacacg catccccgga ttctcacagc
    31081 agaccgcaat atcattaagg tggcgggtgt tacccggagc gccgttactg ttggttgtca
    31141 tgatctttat ctcctgcgct tttgccgtct ttctgaatgt ttttgcgcaa ttcacgccag
    31201 tcacggagtt gttgttctga taaaaaggtc cgcataacat caacgctgat gactgtctta
    31261 ccgctaccgg ttttccccac gaccatcaca ttggtttttc tgtttttcat ccttcacctc
    31321 aggtagtgtt tctgccggat ttccagtacc cgctggcatt ccgtgcaggt tcgtacacca
    31381 ggtatcgttt gctggcgttt gagaggaata tcgttgccac aacattcaca ctcgcagagg
    31441 ctttccccac cccttatgat caactgccgg gcagcctgaa tatgcagccg gttcagcagg
    31501 tcttcctgct tctgtaaatg gtccagaacg tcggccatcg gttctccttg tttacgctgt
    31561 ccggacacgc taccgcccat ccctttcacg tcgttcaggg cactgactgt tccggattgt
    31621 gttgttctgg ccggtattcc ggctcccgtt tactgcgtga caacctggcc tgtagccggg
    31681 tcgacggtgt cccccagcct gaagtcctgc aggtgtttaa gcatgtccgt cgtgttgccg
    31741 ggcatggcag caggcgcaga tgaggctgat ggttgatttt gtttcctgcg tgcagcggct
    31801 ttcgcgcggg cttcttccag cgtttcaagc tggaagccat cctggaaaac cagggtgact
    31861 tcattgcccg caccaatcgg aataacaggg tggtactgtt cagcacgttt gatgtagtaa
    31921 tcagagagtg ttttggccgc agaactgact ccgccgccga gtccggcctg accgatttca
    31981 ccggcactca tgctggcagt ggctccaacg cccacggttg tggaagaggc tttctcaatg
    32041 cccttcccga taccatcaag aaagcctgag ccccccgcat acagcaggat ttgaccatta
    32101 cgcatgacca cttcgccctt gatgccgttc ttgcccataa aggacacatg accggcaatc
    32161 ttctggtcga tattgtcatc acccagcttg cagctgatgg agcgggtacg gacgatggcc
    32221 cgttcactgg aaacatcccc ccaggcttcc agagtcacaa aacacccggt cagatcaaat
    32281 tcctgatcgt taggcatctg aactttgccg gtcagacgaa gctgcatcgg gacggtattt
    32341 ttatcgccgg taacagaggc attagcatca gcaccttcca ccacaatggc ttcggcaaaa
    32401 ctgccggagg aaatccacgg gaaacggggc ttttgcggag tggtgtccgg cagagtgaac
    32461 tcctgactgt ccatcattcc cggacgctga accggggtca tcaccggtgc gacaggagcc
    32521 tgcccaccac cggcgggcca gaatgtggtc gggggcagaa cttcaccggc ctgtaccgct
    32581 gcttttccct gtgaggcgag tgcccctggc aacggttcag cactgagaga gtcaaagttc
    32641 ttcccggctt cgatttgtgt ctgcaggagt ttattttcat cttccagctc acggatacgt
    32701 tcctgagagc ctttcagatc acgcccgata atccccattt ctgtacgcag ctttttaatt
    32761 tcagcctgcg tttccttgtt cagacgctgg gcttccgcaa tggcggaacg ctggacttta
    32821 ttgtcaaagg actgattgac caccccggtc atatccggcg caggttcgcc ctttggtgct
    32881 tttgccggcg gggctttctt caggttcatc tcggatacca tccaggttcc tgccccaccc
    32941 gcaccaatac ccagcacaag cgcgataaac agagcgagct gacgacgacg aacgattttg
    33001 ttaacgttgg ccatctgttc cctccccgtt ttcacggata acggtgacag tcagccgtcc
    33061 cccgcccatc agggtctgtg cattgctgtc aaacatgacg gcacgaactc cgggcttcca
    33121 gaagtcctgc tcacgcagag ccactcccca cgtatttgca ttgcgcagtt cataactatc
    33181 gacccgtaac ttgtcgcccg accatgcttt ctgcggcgtg gcggaaaacc cacccggtac
    33241 gttgataccg tccgcaagcg gtttcacctc gccatagcca tcaggtatcg tgtctgtcag
    33301 cacagcccgg ttgagtgcga tgagcagttt ttcgtaagcc tgagcggttt cccagtggcg
    33361 ggtttctggc cgtgagggag gttctgcact catcagtcgg tagactcgcc cctcaccttt
    33421 gacgggcgtg gccacaacag agaaggtttg ccccagggcc gtttccacat acagcgtgaa
    33481 ggcccgggta gccacggaag tgaacagcac cccaccggca gacgtcagtc gtttatctgc
    33541 cagcatcccg ttctgagctg tgatggctgt gactttatct cccggaataa aaatcatatt
    33601 gggatcggta ttactgatac tgagccggaa ctgccccccc tgcggaaagc tgagtgtggc
    33661 aggtgactgt gcatatactg tcacaggcga agtgatcatc agggcggtgc ccaaaagcag
    33721 cgccggaaag ttatttttca ttggttacct catagaaacg gccaatgcgg gtaaagccac
    33781 cggcgtactt aaggcggaga cgatagtttt ttaattccgt ggtgggtttt gagttgccga
    33841 tccacatttt cagcaccccc ctgacatcca cgcgcccgtc tacgggatag acattgattt
    33901 ccgtggtgta gaatgtggaa ttcacatcac tggcctttat ctgtgcagcc tcttcctgca
    33961 gaactttttt aaattcctct ctgacttctg gttcgacaaa agacatcaga aaagcgtggt
    34021 ttgaatctac cgtttcgggg gagacgttaa gacgcagggc caggaaggac agcgtcatca
    34081 tccggaaaaa ctcggtatcc gcttttgttt cagataccgc gaaaggcgcg ttataggtca
    34141 tcggggtgac gaccgtacgc tgctcatgaa tgagtgttct gttagtcacc gccagccagg
    34201 taacagcaaa ccccagaata aagaccagag ccgtgagtga cagaataata atggcaataa
    34261 tttttgtgga tgagttacga gcgcttattt ccataagatg gccctgtaat attttatttt
    34321 atccatttcc tgtaactgga gtcgggaata acgcggtaga caacccggaa taattcaaca
    34381 ggcagatgcc agtagcacca gttatataac cagcgggaac tctttccgcg ttttgccgcc
    34441 cggattagct gccagaccac tgtcgcaata atcagaccaa atacatattt cttggcgaaa
    34501 aatccccaca gcactaacac agctgcgggg atggcttcat caagcggcag tccgataatg
    34561 cggttctgct ctgacagagt tttcgggaaa cggaattttt ctaaatcgtt tcccgacata
    34621 tcagttgacg aatgtcaggc caacggtggt gaagacaata accacgacga gtcccagcag
    34681 aatcagcagg ttcttggttc ggatatacgt cgcaacaccg ataacgattt cagcgatata
    34741 aatacacatg atgacgaatg agtcggcacc aaaggtcgct tttacatcgt tcttgccccc
    34801 ggccagcagg tcagtggcat gagccatctg ccccatcccc agtaacagca gcggagagaa
    34861 cgcagccaga cgggacagtg cttttggggt gcacactcca cgccatgcca tctggcaata
    34921 atgccatgca cggtttttta cagacgcggg gcgaccgcct tcagataagg ataaattcat
    34981 aaaaactcca ttttgttatt tacaaaaaaa ccagagcggt attgacaggt taagtcaatc
    35041 cggcacagaa ccatcgcgaa tgattctgtg acagaattac tccttcattc ccaaagcctc
    35101 ttcaatttta agcgcagcag tcttgggtat atttctggaa tccttcttgg ctatttttct
    35161 caactcttca taaacgatcc catccacata tacccgaata aacttaccag cagggcgagg
    35221 tttgaggggt atgtttttag tcatttaata acgctccaca catccaaaat ctatatatat
    35281 gttgaactaa aaaaaaagtg gttgtccagt tgcagataaa tctattttca ctactcataa
    35341 cccaatgaaa ataaataact ttagatatgt ataggaaaat tcatgcaacc gcaaattgat
    35401 ctaaatcaac aataaaacgt gacgcagatc acatcacgtt ttaccactaa tcagctcact
    35461 tactgttcag gggtgagttc aattcttagt tcaagaaagg aatgtaaggc cagccttgta
    35521 atgtacaaag ttttaagtgc ctcatttgag ggggaattgt attgatcaca taactctttt
    35581 agcgttttat acaccgcctg atgagttgta ttcctgtaaa cagaaatctc cctgatagtc
    35641 ataccaaaag caaaaagtgt gaaaacctcg atcttgatat cattaatttc aggaaaaacc
    35701 ttgcgtaaaa tttccatacg gataggatcg atgatagcca tcgtgacctc ctgttaaaaa
    35761 tgattgcgtt attggtggct agcgatgaca aggggggcat cccttgtcat cgcgactaaa
    35821 gtaaatcaat ttaataatct acactcgcct ccctggaatt attgattcac cttaaaacaa
    35881 atatatccaa cctcagaacg cccctcaata aattatgata taaacactaa ttttttgaca
    35941 gccatcacaa aaaaaatgac attttcaacc tgaataatta aataaaaatg gtcacactat
    36001 gaatgatgat acaaaaagca accgacagat atgcatcatg attctcaggg ataattaaaa
    36061 agaatggctt gtgttagaat caaacattaa gaggtcgccg cttattatac agagggaaga
    36121 gaaaagcaga attgtaattg cacggcattt aagaaaaaaa catgaaagca ccttgtgcaa
    36181 caaaactcac aaggtgccat gatatataag taactggagt cagtctgtct cctcatcgtc
    36241 atgaaaaaaa atacccacct gctcattaac gaatttttcc atagcgggct taatcttttc
    36301 aatcgtgaaa taaccgctgg caacagactc ctgattgagc atgctcatgt tcataatttc
    36361 actacacata aatctcactc ttgatatatt atccagcatg agtttgttgt actccatctg
    36421 attaaatcca ctttcctttt tttctctctg aattctgtat actctgaggc caagctctat
    36481 aagcattgaa gtaacagatg ataccgtagc atcattttcc gtagccccct cctgcctcct
    36541 ttcagttacc agattctcaa tttcttcacg aacctgattg ctgacaaaag tttgtatacg
    36601 tggcatcgtt gctccttcat gaatcaatta ttatgatcaa tttaaaatat aacctgaata
    36661 ctactatcca tagtataagg cgagtatatc atttcaaaaa acagagtata gaggcgagtg
    36721 tagagcatat caaaaacaga gtacactcta gactatactc tatataacat actgaatttg
    36781 ctgagatact ctaatcagag tatctctata ctctgattag agtataaata gagtgtggtt
    36841 atactccaca ctgagacatc acaatcaaaa agacaccttt acaacaatca taacatactg
    36901 ttttacatga ctaatcatgc aagattaaat cttgcgacgg gtgtgggctt ttgatgttaa
    36961 ggagcaggta tatgaaaata ttcgcaccgg gcaggattca gtattaatgg acgttgccgt
    37021 tttttattaa aaatggcatt ctgactaatg cacaataatg aatatggaat ataaaacatg
    37081 accaaacttc ttcttattgg attactcact ctttcatctc atgttattgc tcaggactgt
    37141 tttgaactgg ctggtagaga ttacaaaatc gatcccgatt tactgagagc gatttcatgg
    37201 catgaatccc gtaacagggt taatgctatc ggcattaacc ctgttacggg atatggctcc
    37261 ggcctgatgc aaatcgactc ccagcatttt aaggaactgt cccgttatgg aatagaaccg
    37321 gaacacctgg taacagatcc ttgcatgaat atttataccg gtgcgtatta cctggcaata
    37381 gcgttcaaaa aatggggagt aacatggaga gcggtcggcg cttacaatgc tggttttcgc
    37441 aacacagagg aacaggatcg gcgcaggaaa acttatgcag aaaaaataca gaacatttat
    37501 cgaaatataa aaaacatgca agggcaatga aatatgttat gccgcgacag cagcatggaa
    37561 ggccagttga tgaatttgag gcataacaga caacacagca atgccgcagc ggaaacataa
    37621 aggtcgaata ttggtgaagg aagactacat cgcattcatg ccgaaacaaa atttaagaac
    37681 ggcattgcat aaccttgcat tggcgatcga atattacaat gaaaaccatc cgcatagtgc
    37741 gttttgttat ctctctccgc gagaatatcg acatcagcgg gtaacgttaa cttaagatac
    37801 aatccctgcc tagaaataaa tgaaaataat accagtaata ttgatatatc actggctata
    37861 acgctgtctg ctgtacaaat gcctttatcc gaatatgaga gtcttcatat actgtattcc
    37921 acgaggacga atccaaatcg gacgacctaa gcggttcagc atatatttat cttaccaccc
    37981 agaaatataa aaatgaaaac tgttattaca ggaagcgata ttgcccaaac aaaaacgatt
    38041 agattatttt tcctgccgat attgaaaact atacctgcca gcaacgctaa aaagaaaaat
    38101 acagctggca cagtccatgt aatggcggga ttatagttta taatgccgaa atcaaagatg
    38161 cattgttcca tagagtaaaa ttcctcctgc tctgtatcca gatcatcatc agtcattgtc
    38221 agatttatcc gctttgctgc ggatgccgat gccgtttttg tgccgcagaa cttacaaact
    38281 ggcgcaaatc cagtggatta atttcctgat ctattttctt ggggagcctt gccataggat
    38341 atactgtttt atgtaaagtg cgtaattata cgcaccttta taactagtag aagttccagc
    38401 attcaggtgc tgcaaattac cgggtcactt gcaagaagaa gcatagctgc atcatgcata
    38461 ccgcgcggat gccagaaaat gccagtagcg tctcctgctg gcatggtccg ttcagacttc
    38521 tgtcgatccg atccacatac cattcagata agcatcaaat gcttcttccc catcccgcac
    38581 cttctgtggc tgaacaagac tgaggctttc atgtgtccag ccgctttcag gagcaggcat
    38641 tttcagcaga atatgcccat cagaatcacg aatctccaga tcagcaaaag cattcccgct
    38701 caggatatct gattcaccgg gtcgttgatc tgtaacatat gtgatatatc ccatccattc
    38761 tctccacatt aaatatttca gcactatact gaacgtcaaa aaaaaacttt cgaattgaca
    38821 cgctcaatct gacgcaggaa ctgtttgaag tgatcggctt ccgcaatgtt gatatggcgg
    38881 aactgcgtat ccacaccccg gctgagcgcg tagctatagc tgaaggccat cttttccggg
    38941 tggcttttgc gatatttttt cagctcagtc aggctgctga attccaggac gtcccactct
    39001 tccccggtgt tttttccctc ccggcaacaa gaatcgtgta cgacgcggtg ttaacgtgga
    39061 tatttcgggt actcatccag ctttctcctc tctctggttt tcagccccca tcatcttttg
    39121 agtttcggga gccgcggttt tgacgttttg cggggacctg ccttttgttc cctccggtat
    39181 tcaccatcac ctttttgaga accttcgccg caaggggcat cactgccacg gcctgacgca
    39241 cctgtcctgg gcaggacgca aaatttttta ccgggtttta ggtaaaaaat tttgtgggct
    39301 gttcatttac cctggacagt cagtgcggca ggttgtggct gtatcagccc ccggcgacag
    39361 gggcttaaac aggtgaatgg caccggacac aacaacgggc agggacccgc cgcaacgacg
    39421 tgaagcgcgg tttgcgaaac ggcgttgcgc cttacatccc ggcggccttt tgccgggatg
    39481 ccgggagcgc aggccacggc gcgacagagg cggcgcgacc ggaacggtcg ctgcaagccc
    39541 ggggtttcgc gtcagggatg gaagcccgtc agggcggaga cagcttcgct ggctccgttc
    39601 acgacagccc gtcccgtcag agggacgccc ggaaaaggca taagcaggag aacaacaaaa
    39661 cagacagtaa tcagggcagc agtaatcaga gtcagagcag taatcagagt cagaacagga
    39721 ggaaaaagag aataatcaca gggaaggtga tgtgatttct gacaggtcat aaacagagaa
    39781 ggcgggggaa aagaacgggc gccgcaggcg cacgttcatc cccgttcccg gagggaaacg
    39841 gccttcctgg cagtctggtt gttcatgccc gcacaatgcc agcaagggga aaggggccac
    39901 cggcgaacca gcagcccctt tataaaggcg cttcagtgca gtcagaccag catcagccct
    39961 gaaaaggcgg gcctgcgccc gcctccaggt tgctacttac cgccggattc gtaagccatt
    40021 gttgccgtaa cttccctgta tccgtccttc agccggactt cgcacaggcg attccgggtc
    40081 aggagtgtga atatcaacaa cgtgcagcac acgattaata cgcaccagat aagggcgttt
    40141 cccggtagtt tcatggttta ctcctccttg caatttgcac ggtaagaggc catactggtg
    40201 ttgatcagac atacagtggg cctcgtgggt taatgaaaat taactacggg gctttctgcc
    40261 ttctgctaca caacacggta acaaaccacc ttcacgtcat gaggcagaaa gcctcaagcg
    40321 ccgggcacat catagcccat ataccagcac gctgaccaca ttcactttcc ctgaaaataa
    40381 tccggtcgtt cagccagttc acgggcaatc agttcatccg caaacagtgg tgcatgcctg
    40441 ttgatacgtt tctccagatt ccgccgtcgt accgcagcct tacgttcttc gctccagcgg
    40501 cgcaccgggc ggtcagaaat aaaatccaga cagaccgcat accctgctcc ggcaacccag
    40561 cgtgacataa cggatgccgg tgcaggctgc cccgcctcca ccacttctga aaccagttca
    40621 cgctcgccag gacagggata aggcagtttc cagcgcagtg agtaacgcca cttcacgccg
    40681 cctcccggag tttgttcggc accatccacc gctccatctc acggacggca tgaccggcct
    40741 cccttgccgc cataccagcc tctgcggtga cctgccagaa tggcatatcc tgccactgcc
    40801 agcgttccat cccggccagt gacggaaaac gctcgcccca ctgccagaac agggtttcca
    40861 ggtcatgctc tgccacccgg tcactccagc gtgcatgcca gcttccgact gtttccggcg
    40921 aatggaacgc cagttcgatt tctgcctgag caaccagcgc ctggtacgca cgggttttct
    40981 gctctgcctc ccgtgcctgc tggcggctgt acttctgcat gaccagtgcg gatttatggc
    41041 gactgcgttc cctgacacgg aaccgcactg acgggaacaa ctgctctgct gcatcgccgg
    41101 gaaggggaag gaggcagacc tcgccctgtg tttcaatcag cacatcgacg gcatacagcc
    41161 actgagaacg gttgccgcgc agttcttctg cggtcaggga cggcatgaaa aatcgcaggt
    41221 cagacagcga tatcctgcgg cttccgttca gacagcggaa aaacgccccc gcatacggat
    41281 attccgccag cgtgttaccc tgatggcggc gtttttccgt ctccgccacc gcccgccatg
    41341 cggcctgttg ctctgcgttc agggggacca gtgcattctg acgggcagtc atgcagcacc
    41401 tccttccgtg accagcaggt tgatgatgct ggcagtggaa tatccggcct catccagact
    41461 cgccgccaga cgcaggatgt gattcaccgt ttcagggcgg aacgatggct gtgtgcgaat
    41521 cacccggaaa ggacgtccgc tgatactgac cagtatcacc atccaggttg cgctggcctc
    41581 accggggctg catacgacaa tatgaaaacg ctcatggaac gggctgaaac gcacctggac
    41641 ggggaaaaat ccgccaaact cgctgccgta ctcgccgttc atcgtccagt tgtccggcgc
    41701 atccagctcc cgcatcacgg cgtgtgtcag ctcacggcgt tcatcacttc cggcagcccg
    41761 gatatcttca tattcctgta cactcatggt ctgtaataca ttcagggtca gttcagtttt
    41821 catcacggta tctcccttgc tgccgccccg tctccggcgg ggcggtgtgg ttgttcatca
    41881 ggcggcatcg gcctggttgt cacggtactc cgtggtttcc gtctctgcct gtggacgtgg
    41941 tgtacacatc cagccaggca cccagcggtt gtctttcata tggaactctg catgttcagc
    42001 cgcatcgcct ttcttcattt tctccgcgtc ccgtgcgaca ccggacagtc ctgcctcatt
    42061 cagggctgcg ataatctgcg gctttttcag atgtccgaag aagtttgctt ttgtcggctg
    42121 ccaccagtcg cgcatgtgaa agccgatggc gctttccagc gaatccagcg gactgcgtga
    42181 cgtgtgacca tgctcgcgag tctggacacc atccacggaa caggctgtac agaaaaccat
    42241 cagattcatc agagtctgtc cgtcaagcgt gaagaatgtg gtgaaatctt tctgccatcc
    42301 ttccggtaac agagcctcca gacgggcttt ctcctgcatc agactcagcc atgcctgacc
    42361 tctctttcca ctgggagcat cacttgtcag ccttctgtga tactcttcaa tatgcatccc
    42421 gaacgggtgg agagttgtat tgcagtagtc aaaaacacaa cagcacaatc gccagaccat
    42481 cagtgccact gttttttcgg gctgctttat cagcgcggcc tggactgcca gcgtgcgttc
    42541 ggaagacatt ttcgtcagta gcggcagact gatttcctcc accgcatcag tcttacgcac
    42601 ctgttccgtg cgttcagcgt cgtcagccac gtcatcttca ctgcgcaact gcacaccacg
    42661 ctggacacat acgttgccat aacgccagga caccaccacg ccacttccgg cacgcatctc
    42721 cggcgtccac gccctgacct tcgccatgca gtcaatcagc ttcatttctg cttccagcag
    42781 gtcggattcc tcacactggt tttccagcgc gtcgtaacga gccatcagtt cgttcaggcg
    42841 ttcttcttct gcctccgtca gcaccgcttc cggctccggc agactgcggt atgttccggc
    42901 atcctcacgg caggaaccaa caggctccat acgtccggcg caccattccc agccttctgc
    42961 ttcccgaagg tgttcagcga cagcctggag tttttccagc agggcagcat cgagcgcaac
    43021 acagtccaca tagccaccct cgtcgtcgct gaacaaatcg gtgcgcagtt catctggcga
    43081 gaaggcatca gcccccacaa agcggaattt agtgttcccc gccaccgcca cttcactttc
    43141 ggtcaccaga cgacgaatgg tctgtacttc cggtttaccg ccccatcccg actgacaggc
    43201 agcttcaaac acctgcacct gacgcgcggt gtcgttctcc agcgccagcg cctgacagtg
    43261 ctctgtggtg atgcggtctt ctgccagcgc atcgaggatg acaggcgcaa ggtctgccag
    43321 tttcagcatt cgctgaacgt ggcggggtga atagcccagc aaatcaccga tttgtgcagg
    43381 cgttttgcct tcctgcgcca ttgcacggaa tccggcaatc tgttcggcag ggtgcatatc
    43441 ccgacgatga ccgttttcag tcattgatgc ggcagtcgcc agttcctgcg gaataacctt
    43501 cacgcggaca ggccagtcag ccggaaggat gttgcgctct gccagcatgt tgagtgctgc
    43561 cagtcggcga ccgcctgcgg cgacaccgta gcggtcacca ggcagggcat gaacaaccag
    43621 attctgtaac aggccaacgc ccttgatgga ctccgccagt tcactgacgg actctgcgga
    43681 atacggcacc gtgcgcacat tcagcggtga tttaatcagt gaggccagcg gcacgctcac
    43741 ttcctccgtc tgcgccagca gggcagacag ggcgttttcc tgttcttttg acgatttacg
    43801 gctggattta cgaccagttt ttgccttaaa ctctgtaact gacatgatta ttcctcttac
    43861 ggtttagtta atgtctgccg gacgtgttcc cgtccggcac ctctcttact taagaatccc
    43921 gaaatccctg atgtaccggt tcatccagta cccggcaccg tcctcaaaat tccaggagcg
    43981 ccagatcaac gtgccatcct gacggacaac caggcgaaaa tgcgttccct gatcatcctc
    44041 gataaagaca ttccggtact gtgcggcaac ggcttcagcc tgttcgcggg taaaggagcc
    44101 gtcaggcagc ccctgaaata ttctgaaata ttcgctcatc cccatctctc cggtgatgcc
    44161 ccgcacagga cggggcggtt gtcacagtca gtcgttcaga acgggatatc atctgaaaac
    44221 ccgtaatcgt caccctccgg cggttgcggc tgaggctccg gctgcgcggc cttacgtcca
    44281 cggccttttg ttttcgcgct cccttttttc gtcgtgtcag cactctgtgg ctgaccattc
    44341 tgctgaggct gcggttgcgc ctgagcgttc tgctgtggtg cactccccag catctgcatg
    44401 gtgcccgtgg tcttaacaag aatttcagtg acgtaacggg tgataccgtt atcttcccag
    44461 ctacgggtac ggagttgccc ctcgatgtag acctgcgcgc ccttgcgcag atattcacct
    44521 gccacttccg cgagcttgcc gaacagcacc acgcgatgcc attccgtttg ctcccgcatc
    44581 tcccccgtct gtttgtcacg ccagctttct gacgtggcca cctgcaggtt tgccactgcg
    44641 cccccgtttg ggatgtaacg gacttccgga tcttttccca gacgaccgac aagaatgacc
    44701 ttgttaatgc cacgaactgc catgatgaat ctccttctgt tgatgttttc agcccccatc
    44761 atcttttgag tttcgggagc cgcggttttg acgttttgcg gggacctgcc ttttgttccc
    44821 tccggtatt
//
LOCUS       DAZFYC010000025        44710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFYC010000025 DAZFYC010000000
VERSION     DAZFYC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.025"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..583
                     /gene="yebB"
                     /locus_tag="ACHKW5_001585"
                     /pseudo
     CDS             1..583
                     /gene="yebB"
                     /locus_tag="ACHKW5_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
     gene            complement(585..1106)
                     /gene="ruvC"
                     /locus_tag="ACHKW5_001586"
     CDS             complement(585..1106)
                     /gene="ruvC"
                     /locus_tag="ACHKW5_001586"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO7510998.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1141..1881)
                     /gene="yebC"
                     /locus_tag="ACHKW5_001587"
     CDS             complement(1141..1881)
                     /gene="yebC"
                     /locus_tag="ACHKW5_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7510999.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1910..2362)
                     /gene="nudB"
                     /locus_tag="ACHKW5_001588"
     CDS             complement(1910..2362)
                     /gene="nudB"
                     /locus_tag="ACHKW5_001588"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO7511000.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2480..4252)
                     /gene="aspS"
                     /locus_tag="ACHKW5_001589"
     CDS             complement(2480..4252)
                     /gene="aspS"
                     /locus_tag="ACHKW5_001589"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO7511001.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4562..5128
                     /gene="yecD"
                     /locus_tag="ACHKW5_001590"
     CDS             4562..5128
                     /gene="yecD"
                     /locus_tag="ACHKW5_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7511002.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5125..5943
                     /gene="yecE"
                     /locus_tag="ACHKW5_001591"
     CDS             5125..5943
                     /gene="yecE"
                     /locus_tag="ACHKW5_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO7511003.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DGRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5996..6391
                     /gene="yecN"
                     /locus_tag="ACHKW5_001592"
     CDS             5996..6391
                     /gene="yecN"
                     /locus_tag="ACHKW5_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO7511004.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6432..7175
                     /gene="cmoA"
                     /locus_tag="ACHKW5_001593"
     CDS             6432..7175
                     /gene="cmoA"
                     /locus_tag="ACHKW5_001593"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO7511005.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7172..8143
                     /gene="cmoB"
                     /locus_tag="ACHKW5_001594"
     CDS             7172..8143
                     /gene="cmoB"
                     /locus_tag="ACHKW5_001594"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO7511006.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8309..10738)
                     /gene="torZ"
                     /locus_tag="ACHKW5_001595"
     CDS             complement(8309..10738)
                     /gene="torZ"
                     /locus_tag="ACHKW5_001595"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO7511007.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(10763..11863)
                     /gene="torY"
                     /locus_tag="ACHKW5_001596"
     CDS             complement(10763..11863)
                     /gene="torY"
                     /locus_tag="ACHKW5_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO7511008.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12251..12997)
                     /gene="cutC"
                     /locus_tag="ACHKW5_001597"
     CDS             complement(12251..12997)
                     /gene="cutC"
                     /locus_tag="ACHKW5_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO7511009.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGYFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(13011..13577)
                     /gene="yecM"
                     /locus_tag="ACHKW5_001598"
     CDS             complement(13011..13577)
                     /gene="yecM"
                     /locus_tag="ACHKW5_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7511010.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13793..15526
                     /gene="argS"
                     /locus_tag="ACHKW5_001599"
     CDS             13793..15526
                     /gene="argS"
                     /locus_tag="ACHKW5_001599"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO7511011.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15568..15612)
                     /gene="yecV"
                     /locus_tag="ACHKW5_001600"
     CDS             complement(15568..15612)
                     /gene="yecV"
                     /locus_tag="ACHKW5_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO7511012.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            15703..16191
                     /gene="yecT"
                     /locus_tag="ACHKW5_001601"
     CDS             15703..16191
                     /gene="yecT"
                     /locus_tag="ACHKW5_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO7511013.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(16311..16703)
                     /gene="flhe"
                     /locus_tag="ACHKW5_001602"
     CDS             complement(16311..16703)
                     /gene="flhe"
                     /locus_tag="ACHKW5_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO7511014.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16703..18781)
                     /gene="flhA"
                     /locus_tag="ACHKW5_001603"
     CDS             complement(16703..18781)
                     /gene="flhA"
                     /locus_tag="ACHKW5_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO7511015.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQRFPGKDEVHVIGLDTPLESLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18774..19922)
                     /gene="flhB"
                     /locus_tag="ACHKW5_001604"
     CDS             complement(18774..19922)
                     /gene="flhB"
                     /locus_tag="ACHKW5_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO7511016.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(20124..20768)
                     /gene="cheZ"
                     /locus_tag="ACHKW5_001605"
     CDS             complement(20124..20768)
                     /gene="cheZ"
                     /locus_tag="ACHKW5_001605"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO7511017.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(20779..21168)
                     /gene="cheY"
                     /locus_tag="ACHKW5_001606"
     CDS             complement(20779..21168)
                     /gene="cheY"
                     /locus_tag="ACHKW5_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO7511018.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(21183..22232)
                     /gene="cheB"
                     /locus_tag="ACHKW5_001607"
     CDS             complement(21183..22232)
                     /gene="cheB"
                     /locus_tag="ACHKW5_001607"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO7511019.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(22235..23095)
                     /gene="cheR"
                     /locus_tag="ACHKW5_001608"
     CDS             complement(22235..23095)
                     /gene="cheR"
                     /locus_tag="ACHKW5_001608"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO7511020.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLEKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(23114..24715)
                     /gene="tap"
                     /locus_tag="ACHKW5_001609"
     CDS             complement(23114..24715)
                     /gene="tap"
                     /locus_tag="ACHKW5_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO7511021.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(24761..26422)
                     /gene="tar"
                     /locus_tag="ACHKW5_001610"
     CDS             complement(24761..26422)
                     /gene="tar"
                     /locus_tag="ACHKW5_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO7511022.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSTFRLAASPLTNKPQTPSRPASEQPPTQPRLRIAEQDPNWETF"
     gene            complement(26567..27070)
                     /gene="cheW"
                     /locus_tag="ACHKW5_001611"
     CDS             complement(26567..27070)
                     /gene="cheW"
                     /locus_tag="ACHKW5_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO7511023.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(27091..29055)
                     /gene="cheA"
                     /locus_tag="ACHKW5_001612"
     CDS             complement(27091..29055)
                     /gene="cheA"
                     /locus_tag="ACHKW5_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO7511024.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(29060..29986)
                     /gene="motB"
                     /locus_tag="ACHKW5_001613"
     CDS             complement(29060..29986)
                     /gene="motB"
                     /locus_tag="ACHKW5_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO7511025.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(29983..30870)
                     /gene="motA"
                     /locus_tag="ACHKW5_001614"
     CDS             complement(29983..30870)
                     /gene="motA"
                     /locus_tag="ACHKW5_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO7511026.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(30997..31575)
                     /gene="flhC"
                     /locus_tag="ACHKW5_001615"
     CDS             complement(30997..31575)
                     /gene="flhC"
                     /locus_tag="ACHKW5_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO7511027.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(31578..31928)
                     /gene="flhD"
                     /locus_tag="ACHKW5_001616"
     CDS             complement(31578..31928)
                     /gene="flhD"
                     /locus_tag="ACHKW5_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO7511028.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            32708..33136
                     /gene="uspC"
                     /locus_tag="ACHKW5_001617"
     CDS             32708..33136
                     /gene="uspC"
                     /locus_tag="ACHKW5_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO7511029.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(33143..34567)
                     /gene="otsA"
                     /locus_tag="ACHKW5_001618"
     CDS             complement(33143..34567)
                     /gene="otsA"
                     /locus_tag="ACHKW5_001618"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO7511030.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(34542..35342)
                     /gene="otsB"
                     /locus_tag="ACHKW5_001619"
     CDS             complement(34542..35342)
                     /gene="otsB"
                     /locus_tag="ACHKW5_001619"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO7511031.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(35509..36495)
                     /gene="araH"
                     /locus_tag="ACHKW5_001620"
     CDS             complement(35509..36495)
                     /gene="araH"
                     /locus_tag="ACHKW5_001620"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO7511032.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(36510..38024)
                     /gene="araG"
                     /locus_tag="ACHKW5_001621"
     CDS             complement(36510..38024)
                     /gene="araG"
                     /locus_tag="ACHKW5_001621"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO7511033.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(38094..39083)
                     /gene="araF"
                     /locus_tag="ACHKW5_001622"
     CDS             complement(38094..39083)
                     /gene="araF"
                     /locus_tag="ACHKW5_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO7511034.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMEVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MEITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            39880..40383
                     /gene="ftnB"
                     /locus_tag="ACHKW5_001623"
     CDS             39880..40383
                     /gene="ftnB"
                     /locus_tag="ACHKW5_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO7511035.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(40462..40713)
                     /gene="yecJ"
                     /locus_tag="ACHKW5_001624"
     CDS             complement(40462..40713)
                     /gene="yecJ"
                     /locus_tag="ACHKW5_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO7511036.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(40827..40913)
                     /gene="azuC"
                     /locus_tag="ACHKW5_001625"
     CDS             complement(40827..40913)
                     /gene="azuC"
                     /locus_tag="ACHKW5_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO7511037.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            41176..41499
                     /gene="yecR"
                     /locus_tag="ACHKW5_001626"
     CDS             41176..41499
                     /gene="yecR"
                     /locus_tag="ACHKW5_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO7511038.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            41670..42167
                     /gene="ftnA"
                     /locus_tag="ACHKW5_001627"
     CDS             41670..42167
                     /gene="ftnA"
                     /locus_tag="ACHKW5_001627"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO7511039.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(42205..42444)
                     /gene="yecH"
                     /locus_tag="ACHKW5_001628"
     CDS             complement(42205..42444)
                     /gene="yecH"
                     /locus_tag="ACHKW5_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO7511040.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            42635..43846
                     /gene="tyrP"
                     /locus_tag="ACHKW5_001629"
     CDS             42635..43846
                     /gene="tyrP"
                     /locus_tag="ACHKW5_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO7511041.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(43908..44573)
                     /gene="yecA"
                     /locus_tag="ACHKW5_001630"
     CDS             complement(43908..44573)
                     /gene="yecA"
                     /locus_tag="ACHKW5_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO7511042.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT        10787 a        11864 c        10685 g        11374 t
ORIGIN      
        1 tgaatatgaa attggagata tcgtctttac atgtataggt gctgccttat ttggtcaaat
       61 atcagctgca tcaaattgct ggagtaatca cgtcgggatc attatcggtc ataacggtga
      121 agacttcctg gttgcagaaa gccgggttcc cctttcaacc atcaccacgc tatctcgttt
      181 tattaaacgt tcgtctaatc aacgctatgc tataaagcga ttagacgctg ggctgacaga
      241 acaacaaaaa caacgaattg ttgaacaggt tccctcccgg ctacgcaaac tttaccacac
      301 cggttttaaa tacgaatctt cgcgccagtt ctgttcaaaa tttgtttttg atatttataa
      361 agaagcgcta tgcattccgg tgggtgaaat agaaacgttt ggagaactgt taaatagcaa
      421 tccgaatgca aaactcactt tctgaaaatt ctggttctta ggttctattc cgtgggagcg
      481 taaaaccgtc acgccagcca gtttgtggca tcatccgggt ttggtgttga ttcacgcgga
      541 gggagttgaa acgcctcagc ctgaactgac cgaggcggta taacttaacg cagtcgccct
      601 ctcgccaggt tcagccgcga ttcgctcatc tgcatcgcat tctgactaac gtggcagtgg
      661 gtgatggcaa tcgccagtgc atcggcggca tccgcttgtg gattagcggg cagtttcagc
      721 aaggtgcgga ccatatgctg cacctggctt ttctcggcgc taccaatgcc taccaccgtt
      781 tgctttacct gacgtgccgc gtattcaaat actggcaact cctgattcac cgccgccaca
      841 atcgccacgc cgcgcgcctg gcccagtttc agggctgagt cagcgttctt cgccataaag
      901 acttgttcaa tggcgaaata atcaggctgg aactgggtga tgatttccgt cacgcccgca
      961 tagatgagct tcagacgaga cggtaaatca tccactttgg tgcgtatgca tccgctaccc
     1021 aggtaggaca gttgcctgcc tacctggcga attacgccat agccggtcac gcgcgaaccc
     1081 ggatcaatgc cgagaataat agccatcacg cgtctccgtt ttgctgttta gcaggcctca
     1141 tcagagagtc gctgcgacct catcagagat ttcaccgtta tggtaaactt cctgcacgtc
     1201 gtcgcaatct tccagcatat cgatcagacg catcagtttc ggtgcggttt ctgcatccat
     1261 atcagctttg gtggacggga tcatggaaac ttccgcgctg tctgctttca gacctgccgc
     1321 ttccagagcg tcgcgcactt tacccatttc ttcccatgca gtgtagacat caatcgcgcc
     1381 gtcatcatag gtcacaacgt cttcagcacc tgcttccaga gctgcttcca tgatggtgtc
     1441 ttcatcgcct ttctcgaagg agatcacgcc ttttttgctg aacagatagg ctacggaacc
     1501 atctgtaccg aggttaccgc cacatttgct gaatgcatga cgcacttcag caacggtacg
     1561 gttgcggttg tcagacagac attcaatcat gattgccgtg ccgccaggac cgtaaccttc
     1621 gtagatgatg gtttccatgt ttgcatcatc atcaccgccc acaccacgtg caattgcgcg
     1681 gttcagtgtg tcacgggtca tgttgttaga cagtgcttta tccaccgccg cacgcagacg
     1741 cgggttagcg tccggatcgc caccgcccag cttagccgcg gttaccagct cacgaatgat
     1801 tttagtgaag attttaccgc gcttagcatc ctgcgcagct ttacgatgtc tggtgttggc
     1861 ccatttacta tgacctgcca taaaaatatc tccagatagc cctgcctgtt caggcagcgt
     1921 taattacaaa ctgttcaatc gcctgccggt tgctccagga cttagtgagc gccgccgcag
     1981 caggcgcatc aagccacttg taagccagat gttcagtgaa aacgatctgc cgctcgtgcg
     2041 gaagcgcaag acagaaccat gattccgtat tacgcgtcac gcccggcgca tagcgatgac
     2101 gtaaatgtga aaaaatttca aactctaccg tgcgctgaca gtcaattaag gtcagttgtt
     2161 cagcgacaac atcaatggtg acctcttcct ttacttcgcg catggcagct tgcggcgcgg
     2221 tttcaccctc ttccacgctg ccggttaccg actgccagaa atcgggatcg tcacgccgct
     2281 gcaacatcag cacccgtttc gtatcttgtg catagatgac cactaagatc gaaacgggac
     2341 gcttatacac tttatcctcc acgctgcctc ttcgttgact gccttcgctc atcccattca
     2401 catagttatc tatgctcatg ggagttcact cagttgccgc cttgatgcaa cgcgaatgat
     2461 ttcgtgtatt tgagtcatat cagttattct cagccttctt cacaacctga atgctcagct
     2521 cagccagtgc agtcgggtta gcaaagctcg gtgcttcagt catcagacac gctgccgccg
     2581 tggttttcgg gaaggcgata acgtcacgga tattgtcggt gccggtcagc agcatggtca
     2641 gacggtcaag accgaatgcc agacctgcgt gcggcggagt accgtatttc agagcgtcga
     2701 gcaggaagcc gaatttctcg cgctgttcct cttcgttgat acccagaata ccaaacaccg
     2761 tctgctgcat atcaccatta tggatacgta ctgaaccacc gcccacttcg taaccattga
     2821 tgaccatatc gtaagcgttc gccaccgcat tttccggtgc agctttcagt tctgcagcag
     2881 tcatgtcttt cggtgaggtg aacggatggt gcattgccgt caggccgcct tcaccgtcgt
     2941 cttcaaacat cgggaagtcg ataacccaca gcggtgccca tttgctttcg tcggtcagac
     3001 caaggtcttt acccactttc aggcgcagtg cgcccatcgc gtcggcaaca attttcttgt
     3061 tgtcggcacc gaagaaaatc atatcgccat cttgcgcggc agtacgatcc aggatggctt
     3121 cgatgatttc tgcattaagg aacttcgcta ccgggctgtt gataccttcc agacctttcg
     3181 cgcgttcgtt aactttgatg taagccagac ctttcgcgcc gtagatttta acgaagttac
     3241 cgtattcgtc gatctgctta cgggtcagcg atgcgccgcc cggaacgcgc agagcggcaa
     3301 cgcgaccttt cggatcgttc gccggacctg caaatactgc aaactcaaca gatttcagca
     3361 gatcagcaac gtcggtcagt tccatcgggt tacgcagatc cggtttatca gaaccataac
     3421 ggcgttctgc ttccgcaaag gtcattaccg ggaaatcgcc cagatccacg cccttcactt
     3481 ccagccacag atggcgcacc agcgcttcca tcacttcacg cacttgcggc gcggtcatga
     3541 aagaagtttc cacatcgatc tgagtaaatt caggctgacg atcagcgcgc aggtcttcgt
     3601 cacggaagca tttaacgatc tgatagtagc ggtcaaagcc ggacatcatc agcagctgtt
     3661 tgaacaactg cggggattgt ggcagcgcgt agaatttacc tttgtgcaca cgagaaggca
     3721 ccaggtagtc acgcgcgcct tccggcgtgg ctttggtcag catcggagtt tcgatgtcga
     3781 ggaagccgtg gtcatccata aaacggcgca ccaggctggt gattttagcg cgggttttca
     3841 ggcgctgagc catttccggg cgacgcaggt cgaggtagcg gtatttcaga cgcgcttctt
     3901 cggtgttgac gtggttagag tcaagcggca gaacatctgc gcggttgatg atagtcagcg
     3961 aggacgccag cacttcgatt tcgccggtcg ccatatcgcg gttaatattt ttttcgtcac
     4021 gcgcacgtac ggtgcccgtg acctgaatgc agaactcatt acgcagttca gaggccagct
     4081 ttaacgcgtc cgcacgatcc ggatcgaaaa atacctgcac gataccttcg cggtcgcgca
     4141 tatcgatgaa gatcaggcta ccaagatcac gacgacggtt gacccaacca cacagagtca
     4201 cctgctgccc cacgtgggac aaacggagct gtccacaata ttctgtacgc atgagatatc
     4261 ccttaactta gctgccggcg gatgccccct gctgcgcagg tgaccaagtc gcagcgttag
     4321 ctgtatgtca caactgaatg aaaaaaggcg gctattatac tggaaattct gccgcaccgt
     4381 aagagcctgg cccgcgctgg aacgcctcgt taccacttta tatcgggcct gaaatcagac
     4441 tctacgccag tttgctataa aggtgttgcc cgaactcata aaaattaaca aaatttgtcg
     4501 ttccgccatc ggctaatcgc attaaggtgt gaggcacgat tttgtttttt caggagtcat
     4561 tatgcttgaa cttaatgcta aaaccactgc gctggtggtg atcgatttac aagaaggcat
     4621 cttacctttt gccggtggtc cacatactgc cgatgaggta gttaatcgcg ccgggaagct
     4681 ggcggcgaaa tttcgcgcca gcggtcagcc cgtgtttctg gtgcgcgttg gctggtctgc
     4741 cgattacgcc gaagcattaa aacagccggt tgatgccccc tcccccgcta aagtgttgcc
     4801 cgaaaactgg tggcaacatc cagctgcatt aggtgcaact gacagtgata tcgaaatcat
     4861 caaacgtcag tggggtgcgt tttacggtac ggatctggag ttgcaactgc gccgccgagg
     4921 tatcgataca atagtgttat gtgggatctc gaccaatatc ggtgttgaat ccaccgcccg
     4981 caatgcctgg gaacttggct ttaatctggt gattgccgaa gacgcctgta gtgccgctag
     5041 cgccgagcag cacaataaca gcatcaacca tatctaccca cgcatcgccc gtgtgcgtag
     5101 cgtggaagag atccttcacg cgttatgatt tacatcggtc taccgcaatg gtcgcatcct
     5161 aaatgggtgc ggttggggat caccagcctt gaagagtatg cccgccactt taactgcgtg
     5221 gagggcaaca ccacgcttta cgccctgccg aaacccgagg ttgtcctgcg ctggcgtgaa
     5281 cagaccacag atgacttccg cttctgtttt aagtttccgg cgaccatttc gcatcaggca
     5341 gcattacgac attgcgatga tttagtgact gaatttttga cccgcatgtc accgttggct
     5401 ccgcgcattg ggcaatactg gctgcaactg cctgccacat tcggcccacg ggagctgcct
     5461 gcgctttggc attttctcga ttctctccct ggcgaattta attatggtgt ggaagtccgc
     5521 catccacagt ttttcgccaa aggggaagag gaacaaactc ttaatcgcgg tttacatcag
     5581 cgcggcgtta atcgggtgat tttagacggc cgcccggttc atgcagcatg tccacacagt
     5641 gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc tgtactgacg
     5701 gcgacaaatc cgctgatccg ttttatcggt agtgatgata tgacgcaaaa ccgggaatta
     5761 tttcaggtct ggttacagaa attagcgcag tggcatcaga ccactacgcc ttatcttttt
     5821 ttacatacgc cagacattgc ccaggccccg gaactggtac ataccctgtg ggaagactta
     5881 cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc ttctcttttc
     5941 tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag tttgtatggt
     6001 aagcgcgcta tatgccgttt taagtgcgtt gttattaatg aagttctctt ttgatgtcgt
     6061 tcgcttgcgt ttgcagtacc gcgttgccta tggtgacggt ggttttagcg aactgcaaag
     6121 cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg tgctgatgct
     6181 gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca tcgttttgct
     6241 tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct ggcgacgttc
     6301 cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc tttggtatat
     6361 gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc tttttcccgg
     6421 atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc agactgggcg
     6481 actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag cgttccgttc
     6541 ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc gttcaacctg
     6601 gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg gtgcgtcgca
     6661 acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg atgattgaac
     6721 gctgccgtcg tcatattgac gcctataaag cccctacgcc agtagacgtc attgaaggtg
     6781 atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt accctgcaat
     6841 tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga ctgaacccag
     6901 gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt ggtgaactgt
     6961 tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg gagatcagcc
     7021 agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc cataaagcac
     7081 gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt aactttggtt
     7141 cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact tttattctct
     7201 gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga ttgctaactg
     7261 gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacgcggtgg aatttctgcc
     7321 tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa gcgaagagcc
     7381 actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga tgccatggcg
     7441 caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt ccgactggaa
     7501 atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc tggatgtcgg
     7561 ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc tcgcggtggg
     7621 gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac tactgggtaa
     7681 cgatcagcgc gcacatttgt taccgttagg tattgaacag cttccggcac tgaaagcctt
     7741 tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg agcatctctg
     7801 gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc tggttattga
     7861 tggcgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc gtaatgtcta
     7921 tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt ttgtagatat
     7981 ccgcgttgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg aatggatggt
     8041 taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag ttgaaggtta
     8101 tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg gcattactgc
     8161 cggatgcggc ttgaatgcct tatccggcct acaaaccctt gctaattcaa tctattgcag
     8221 ggactatgta gatactgata agcatagcgc atcaggcagt tttacgtttg cataacctca
     8281 gcgcccgttt ccgggcgcta ttcacttctt atgcctgaat agctggctga tcaaacgccg
     8341 ttaacttcgg cgcattgccc gtatattttt cgatatacac cagcgcagag ttaccggcac
     8401 aggcatttgc cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt
     8461 tacacaagcc attttccaga tctggccatg caccttcatg cacgcatacc acgccttttt
     8521 tgatcccgtc agtcaccacc gcgcctgtca gaatctgacc acgtttgttc cacacacgca
     8581 ccagatcacc attcgcaata ccaaagcgag cagcatcttc ggtgtgaata gtgattggtt
     8641 cacgatctgc aaccgcatat tttttacgca gttccgcata gttaagctga ctatgtaaac
     8701 ggtgtgccgg atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact
     8761 catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg
     8821 ttttggagta aatctcaatt ttgccgcttg gcgtacccag cgcatttttc accggatcgg
     8881 cgcggaaatc agcataacga acgtactgtt cgttcttctc gctgcggcgc atttcgatca
     8941 gtttattttg ctgccagaag gcattaaaca tcggcatagt cacgcgttgc gcacgagcac
     9001 ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat
     9061 agatctcttt tccgccaggt ttcagtaatt cagcaagatc ggcaaatacg tcaaaatcgt
     9121 tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt
     9181 aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat
     9241 ctgcatgttt agcagctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct
     9301 gccatgcttt gatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc
     9361 accagatcat tttgatattc ggatacgttt gttctttacc gttatgctga tatttgccac
     9421 caggattttc cagcgcatcg acgatacggg caacaggaat cgccgtcatt ccgccatcat
     9481 cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa
     9541 cacgcgtcgg attgccaccg ttggagtaat gataagagaa accaaaaccg ccgcccggtg
     9601 taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat
     9661 attgctggcg ctgaattccc cagccagcca tcagcattgt gcgattcgcc gccatcagtt
     9721 cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc
     9781 tcttcggagt attatcgctc ttacctgtca gatactcttc aaactgcgga tacccggtgg
     9841 tgtatttttc aaggaacact ttatcgtgtt taccttgtgt catcagggta tgcgcaatcc
     9901 ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtagca ttatcaccaa
     9961 agaactcgat ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt
    10021 tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg
    10081 tattcagcgg gttcatcccc cacagcacca cagcctggct gttttccaga atcagcggcc
    10141 aggaggtctg ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg
    10201 cagccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac
    10261 gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg
    10321 caaaaatagc agatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt
    10381 ttaatgcttg ctcccagctc acctgtacat aagtatcttc gccacgacct ttcgccggtt
    10441 gcagtggatt atcgagatag ctttttctta ccatcggatg ctgaatacgt gccgtggtgt
    10501 gtacctgatc cgccgccgta gactgtaagg agttcggtat ggttttcgcc agcgcgcctg
    10561 ttgaagaaac aatcttgccg tccttcactt ctacgttcat cgctccccaa cgtcccgcgg
    10621 tgaggatttt accgcccttc tcttctgccc atgcggggag cggtgctgcc gatgtcacca
    10681 ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt aatgtcataa
    10741 cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg ttgtactgga
    10801 aataccgcgt taaaatgtcc agttcgtttt cgctcatgct ggttcgcgca cccattcctt
    10861 tggcaatgga cggccatgca ttgacggtgt aatggtcagc ggcaataggc gcatggcaac
    10921 cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcggttta cggtccgcta
    10981 atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg ccgtattcgt
    11041 cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt aatgtggcta
    11101 gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc atttgataac
    11161 cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga tcggtaccag
    11221 gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata tgtgcgccat
    11281 tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca tccattttta
    11341 tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg ccgccctttt
    11401 gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc atggcatcaa
    11461 aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc catactgttt
    11521 cggccatttc ctggcgatga gtttcgaact tatcgtcact gtctattttg ccgctaacaa
    11581 attcatgata aatatcttta gatgctttta atttagcaaa taaataatcc atccctgact
    11641 ttggaatatg gcaatctgca cattccgcac gtatcccttt ctggttcgaa aagtggacag
    11701 ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa aatgctgtat
    11761 ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc gccgccacca accacaaccg
    11821 ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg catagattcc tctaaataaa
    11881 ttccagaaca ccgaaatcat tgtataatta aatacacaac gaattaaccg cttgcataac
    11941 tcggcacaac actgcctgaa ataatcgata aagaatatga tttattacaa tgtaatcatt
    12001 aattgctaag gaataaccca gttgctattt agaatatata tttatcagtt ttagcaattt
    12061 aaatcccata atcaatgtga atataaaaaa tattattagc acaacgctat gttcactgtc
    12121 acccgctcaa cagatcacgg ttatcgttcg ttttttatac tgctcaggga taaaaaaagg
    12181 cccctgttga aattgcaggg gcctggtacg agcaagcatc atattgggcg acatgatgca
    12241 acggtaaaaa tcatttggcc tgatggcgtt caatgattcc tttcatttca gcaaccgccg
    12301 ccccgtctac gatataacgc gaatactcgt ccgcgtgttc atctgatgac atggacaatc
    12361 cttgattacg ataacgcatc ggtgaggctt gccacgctcc cgcggagcta tggacttcca
    12421 gcactccggc atcgaggaag tggtgcaggt tttctgcacg gactcctgct ccggccataa
    12481 tgattggagc atcacgatgg gcaataagtt ccataatttt tgataaacct tgcagtgcgt
    12541 ctgatttttg ccctgatgtc agtacccggg caatgcccaa ttccgcaaga ttattgagtg
    12601 tatataaagg gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg
    12661 cagcagccat tattttttcc attcgtggca tatcgacatt cccgtcaaca tcgagaacgc
    12721 ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca agaatggcgg
    12781 caaactcacc gtcgctgtag caaaaataac caccgcgtgg gcgaattatc ggatgcacag
    12841 ggatcgtcac ccgctggcgc acggatttca gtacccccaa cgacggcgtt aagcccccct
    12901 cttttggggc tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac
    12961 attccatgct gtaacagcaa atttccagta atgccatttt tgctccttaa ttacgctgac
    13021 tgctcgctgg caacgatctc ttcaatggac cacggatgaa acttaatagt tgttttgcca
    13081 tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct caccttttgg tgaactggtt
    13141 ttaactgaga tccccggtag gctgagtcct tcatcagaaa gcaatgccag cgcacgggca
    13201 ttcagcgttt ctggatcacc cggcagaacg atttcaatat gttcccaacc ttcgtgtggg
    13261 taacgttttt ccccgggcca cggtagctcc acaatagaaa actgccagtg cgcaacctgt
    13321 accggttcat gcaatttaaa cagacaaatc ggtctgccat tgatcatatt ttctgacaaa
    13381 agctcgccac actgttcaaa cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa
    13441 cgcaaagaaa tgtgatcggc agtcagcgga gtgatattca accccagacg gagggaaagt
    13501 tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata
    13561 gattgccagt tcgccataat cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt
    13621 gccctgcgcc aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa
    13681 tacgccacgc gcacgttgct gacgaaaaca gccatttgca gtatactccc gccctaattt
    13741 ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc cggtgaatat
    13801 tcaggctctt ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga
    13861 ttgcgaaccg caggttcgtc agtcagcaaa agttcagttc ggcgactatc aggctaacgg
    13921 catgatggca gttgctaaaa aactgggtat ggcaccgcga caattagcag agcaggtgct
    13981 gactcatctg gatcttaacg gtatcgccag caaagttgag atcgccggtc caggctttat
    14041 caacattttc cttgatccgg cattcctggc tgaacatgtt cagcaggcgc tggcgtccga
    14101 tcgtctcggt gtttctacgc cagaaaaaca gaccattgtg gttgactact cagcgccaaa
    14161 cgtggcgaaa gagatgcatg tcggtcacct gcgctctacc attattggtg acgcagcagt
    14221 gcgtactctg gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg
    14281 cactcagttc ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa acgccggtga
    14341 aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt acgacgaaga
    14401 tgaagagttc gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg gtgacgaata
    14461 tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc agatcactta
    14521 cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc tctacaaccc
    14581 aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag aaagcgaagg
    14641 ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa ggcgaaccga tgggcgtgat
    14701 cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct gtgcgaaata
    14761 tcgttatgaa tcactgcatg ccgatcgcgt gctgtattac atcgactccc gtcagcatca
    14821 acacctaatg caggcatggg cgatcgtccg taaagcaggc tatgtaccgg aatccgtacc
    14881 gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt tcaaaacccg
    14941 cgcgggtggt acagtgaaac tggccgatct gctggatgaa gccctggaac gtgcacgccg
    15001 tctggtggca gaaaagaacc cggatatgcc agctgacgag ctggaaaaac tggctaacgc
    15061 ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca cggactacat
    15121 cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca tgcagtatgc
    15181 ttacacgcgt gtattgtccg tgttccgtaa ggcagaaatt aacgaagagc aactggctgc
    15241 agctccggta atcatccgtg aagatcgtga agcgcaactg gcagctcgcc tgctgcagtt
    15301 tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtgatgt gtgcttacct
    15361 gtacgatctg gctggtctgt tctctggctt ctacgagcac tgcccgatcc tcagcgcaga
    15421 aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga agacgctaaa
    15481 gctgggtctg gatacgctgg gtattgagac agtagagcgt atgtaatcga tttttcgtga
    15541 gagtaaagcc tgatcagggt tacctgatca ggctttttta ttgccatcta aatgtattct
    15601 gaaaatggac atgccattgt tttctgactg ttggataaga ggccagaagc gtaatatccg
    15661 gccccaggga aacgataacg gttgaattta aggaataccg cagtgtttaa atttcttgta
    15721 ttaacattag gcattatctc ttgccaggct tacgcagaag atacggttat agtaaacgac
    15781 catgacattt cagccatcaa agattgttgg caaaaaaatt cagatgatga tactgacatt
    15841 aacgtgatca aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca attaaataaa
    15901 gcctatggtg aagcttatcg ttatatagaa caagtgccac gcacaggtgt aaaaaaacct
    15961 gataccgaac aacttaactt gcttaaaaaa tcacagcgag cctggctgga ttttagggac
    16021 aaagaatgtg aattaatcct ttcaaatgag gacgttcagg atttaagtga cccttattct
    16081 gaatcagaat ggctctcatg tatgatcata cagaccaata cgcgaactcg ccagttgcag
    16141 ctataccgta actctgaaga tttttatcca agccctttga caagaggata attcacatct
    16201 ttttggcatg ttttgttgca agctattcct gataaataat tgcaacaaga catcgagcct
    16261 ttttcactga gttattaaac atactcgcga gcgcgtaatt tttttgtcct tcagcgataa
    16321 ttcacaatca cttcattacg ttgtaccttt agcggtggaa ttaaccgccc accgcctggc
    16381 acttcccaga taaatcgcaa cggttctgct gccgctatac cggaaaaggc cacggtggtt
    16441 ccgctctgcc cctctaattc gacacaacga gattgcgaac acaagcgaac ccgcagtcct
    16501 gaaggtgtcg ggccgataag ctgataacgc cacgctacca gcgtcatcaa tcctgaagca
    16561 ggttgtcgcg tagaaagagg cgcagacgac atcgactcac cgcgatgatt tagcgtaatt
    16621 cctacactac ttgcctgcca catcccctcc ccggcggctt gcaccagcag cggaaacaat
    16681 aatattgcta ataaggttct cattatttgc cgccaattgt cgccgtcatg cggatatgtc
    16741 ggttatcaga cagttccaga ttcgacagga ccactaactg cggcaagctg cggcgcagga
    16801 agcgagacaa taatggtcgc agcgcgtggt tcaccaacaa tactggcggc gcacccagca
    16861 tctcctgacg ggatagcgct tcctgagttt gcgccagtaa acgatccgcc agccctggct
    16921 ccagtcctcc cccgccctgc agcgcctgta gtaacaaact ttccagcggt gtatcgaggc
    16981 caataacatg gacttcatct ttgccaggaa acctctgctg ggtaatcgcc cgtcccaacg
    17041 ccacgcgcac gacggcggtt aattcatgtg gatcgctttg gatgggcgca tgttccgcca
    17101 gcgtttcgag aatggtgcgc atatcgcgaa tcggtacttt ttcatcgagg agattttgca
    17161 gcactttatg cagtgtggtg agcgtgacga cgccaggaac gagatcttcc gtcagctttg
    17221 gcatctcctg ggcgacgcga tccaacagct gttgcgcctc ctgacgacca aacagctctg
    17281 cggcatgctg gctaatgagg tggttaagat gcgttgctac caccgtgctg gcctcaacca
    17341 ctgtgtaccc ctgaatctgc gcctgttctt ttagcgcact ttcaatccag atagcattca
    17401 ggccaaatgc cggatcgacg gtcgcctcac caggtaacgt cccggcagcg gttccagggt
    17461 taatcgccag ccagcgcccc ggataagcat caccactgcc aatctccacg cctttcatca
    17521 aaatgcgata gcgggcaggt tgcagatcca tattgtcgcg aatgtgcacc actggcggca
    17581 gaaatcccat ctcctgggca aatttcttgc ggatactgcg tatacggccc aacaactcac
    17641 catcctgctg gaaatcgacc atcgggatca gtcgataacc cacttccatt cccagagaat
    17701 cttccagttg tacatcgttc cacgtcgctt cgacaacggt attattctct gccattttta
    17761 ccggtttggg ttcgacaggc gctttttgtt cgcgtccgcg tatccaccag gccagcccca
    17821 gcaatccggc agtgaacagc aaaaatacca ggttcggcat tccaggcacc aggccgagta
    17881 aaccgagcac ggcggcgctt aacaacataa cgcttgggtt actgaaaagc tgattcacca
    17941 tctgctcgcc aacatcctga tcggtgctga cacgcgtaac gatgaccccc gcggcggtag
    18001 aaatcaccag cgccggaatt tgtgccacca gaccgtcgcc aatggtcaat agtgtataac
    18061 tttccgccgc gtgtcccatg ctcatgccat gttgcagcac gccgaccagc aacccgccga
    18121 caacgttaat gaccatgatg aggatcccgg cgatggcatc gccgcgaaca aacttacttg
    18181 ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg cgttttttcg
    18241 cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc tgcttacccg
    18301 gcataccatc gagaacaaag cgcgcaccca cttctgcgat acgcccggca cctttggtaa
    18361 tgaccataaa gttgatgatc acgagaatga caaacactac gataccgata gcgaaattgc
    18421 caccaacgag gaagtgaccg aatgcttcga ccaccttccc agccgccgcc gcgccggtat
    18481 gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc aacaacgtag
    18541 taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta aacatcgcca
    18601 ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg agtatgaatg
    18661 cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt ccggcaagga
    18721 tctgccattg tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc agattactca
    18781 tgggtcggtt tctcgttaat aaaatccagg gcttccggca ccggaagatg agtaggttgt
    18841 acagggcgct gtccaccagc cagacgccag cgtttcagtt gccagaccca ggccagcact
    18901 tccgccaccg cggcgtacag ttgacccggg atttgttgac caatctccgc atgtcgatac
    18961 agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc gccaatttca
    19021 cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc gctcattttg
    19081 ttttcgtcat actgcaacgc taccgaatag tgggtcgggt tattgacaat gacatccgct
    19141 ttcggcacat cggccatcat ccgacgccgt gcagcagctc gctgcatctg acggatccgc
    19201 cctttaacat gagggtcacc ttcgctttgt ttgaactcat cacgaatatc ctgccgtgac
    19261 atacgcagct ttttcaggtg gctgaatatt tggaaaaaga cgtcaaatcc caccattgga
    19321 atgacaccaa gcaccaccag cagtgcgcat agccctacca aatccatcgc attacccatg
    19381 gcggtaatcg gagactcggc catcaagcgc atcatctgcg gccagtgatg ccagagaaaa
    19441 aaccccgtca cgctgccaac caggatggtt ttcaaaattg ctttaagcaa ctccgcgcca
    19501 gtctgagccg agaacatccg tttaatgccc ggtagcgggt tgagtttgga aaacttcggc
    19561 tgcaaggatt tgccgctaaa taccagccct cccagcatga ccggagaaat gagcgccacc
    19621 agcaccacgc cgctaatcag cggcagcagc gccagcatgg cttctctgat cagcagaata
    19681 atctgcccga ggatcagatt cgggtcattg ataatactgt gatcaaaatg cagcccagcg
    19741 gagagcatgc ccgacaatcg acgggccagc gacacaccgc caaaccagat aacactaacg
    19801 cccaccagca aaatcagcag tgaggtcagt tcacgggaac gcgggatttg cccctcttcc
    19861 cgcgcttttt ctagtcggtg aggtgtgggg gcttctgttt tgtcgtcgct ctcgtcagac
    19921 acgtcgccaa tcctggaaag agtattaagc gtgaatgatg ccagagcgca aagcgttcaa
    19981 tggtttgagt aaggggcaaa acaggcggga tttagggctt ttgctgccac acatcaagca
    20041 tagtgtgcgt ttgtcggatg cggcgtcatc gccttatcag accgcctgat atgacgtggt
    20101 cacgccacat caggcaatac aaatcaaaat ccaagactat ccaacaaatc gtccacctga
    20161 tcctgactgg ctaccacgcc ggctttgctg gtatcgacct gaggtccatt aagcaaactc
    20221 tggttttcac gttttggacg cgactcctgt tccgggatgt tttccaacag caccatcagc
    20281 aactggcgtt cgatctcctg aatgacatcc atcatccgct taatgacctg cccggtgaga
    20341 tcctgaaaat cctgcgccat catgatttcc agcagttgcg cgttagtaaa gctggtatgc
    20401 gcgggtacat ctgccagaaa ttgtcgtgta tctgttacca gttcacgggc gtcggcaagg
    20461 tcaatcggat cggcaaacca gtcatcccaa cgttgggtta acgcttttgc tgatttctcc
    20521 atttgatcct gatgcggttg tgacgcctca acactgttca gcgcccgctc cgcagcctgg
    20581 gcggtcatct gcacaacata gtacaaacga tcgcgcgcat cggggatggc ttccgccgct
    20641 tcggcaatgg cctgatccag ccccagttcc cgcaaactgt cgcgcagcat acgcgtcagg
    20701 ctgccgatgc gcgcaatgat atcgccagct gaatgctcgt cagcaggttt gattgatggt
    20761 tgcatcatag tcgcatcctc acatgcccag tttctcaaag attttgttga gtttttcctc
    20821 cagcgtcgcg gcggtaaatg gcttcaccac atagccactg gcccccgctt gcgccgcagc
    20881 aatgatgttc tctttcttcg cttctgcagt caccattaac actggcaatg ccgacatcgc
    20941 gccatccgca cgaattgttt tcagcaattc caggccatcc atattgggca tgttccagtc
    21001 ggagataaca aatccataac cgcctgcctg caacttattg agagcgtcga cgccatcttc
    21061 cgcttcctca acattattga atcccagctc tttaagcagg ttacgcacta tgcgtcgcat
    21121 ggtggaaaag tcatccacaa ccaaaaattt aagttcttta tccgccattt cacactcctg
    21181 atttaaatac gtatcgcctg tccggcacta atttttgcca acatttgctg gcttacctgg
    21241 ctaagatcga ccacttcgca gacaccaccc atattgatgg cctcgcgcgg catgccgaac
    21301 accacgcaac ttgcttcgtt ttgcgcaagg gtccatgccc ccgcctgacg catcgccaac
    21361 attcccgccg cgccgtcgtt gcccataccg gtcaggatca ccccaaccgc attacgcccc
    21421 gcctgtttgg cgacagaatg gaacaacaca tctaccgaag gccgatgacg gttaaccgcc
    21481 gggccatcgt gaattttgat ttggtaattt gcgccactac gcgacagctc catatgccga
    21541 tcgcccggcg caatataggc atgccccggc aagacacgtt ctccgtcttc ggcttcttta
    21601 accccgatct ggcaaagctt attaagtctg tcggcaaaag agcgggtgaa accgggcggc
    21661 atatgttggg taattaacaa tgccgggctg gaaagcggca acggttgcag tacgtgacga
    21721 attgcctcag ttccacccgt tgaagcacca atcgcaatca gtttttcaga actcaacaac
    21781 ggacccgcct tcagcgttgt cggtgctgac aatggcttat gtgctgcaag gctcgccttt
    21841 gctgccgtac gcaccttttc agcaatcatt tcgctatacg ccagcattcc ttcgcgaata
    21901 cccagttgcg gtttggtgac aaaatctatt gcccccagct ccagcgcgcg cagcgtgact
    21961 tctgatcctt tgccggtcaa ggaagaaacc atcacaacgg gcattggacg caaacgcatt
    22021 aatttttcga ggaaatccag tccgtccatc cgcggcattt caacatccag cgtcagcaca
    22081 tcgggattga atttcttaat caagtcacgc gcgaccagcg gatcaggcgc ggtcgccacc
    22141 atttccatgt cgctatggct gttgatgatt tctgtcatga tctggcgcat cagtgccgaa
    22201 tcatcgacag ataacaccct gattttgctc atcgttaatc cttacttagc gcatacaccg
    22261 tctgaccacg cagcgtgaag cggcgctcaa ggtggctaaa gttttcagag tgacccgcaa
    22321 acagcaatcc gtcgggttta aggagcggaa caaagcggcg caaaatctcc tgctgggtat
    22381 tttgatcgaa gtagatcatg acgttacgac agaagatcgc atcaaacggc cccggcacgg
    22441 tgtactgttt ctccagtaga ttcagcgggg caaaatcaac atagttcgcc aactcctgac
    22501 gcacgcgtac cagcccttca tgcggtcccg tccctcgcat gaaataccgt tgcagttgct
    22561 gcggcgtcag gtttttcaac tcttcatggc gataaatacc gcttctggct ttttccagca
    22621 cttcggtgtc gatatcactg gcaaacactt tccagcgtcc gggcgcggtg cccaatgtgt
    22681 cagccagcgt catcgcaatg ctgtacggct cttcgccggt cgaagccgcc gcgctccata
    22741 cgcgatactc gccagaacgg cgacgtgcgt gatccgcgag cagagggaaa tggtgtgcct
    22801 cacggaaaaa tgccgtcaga ttcgtggtca gcgaattgat aaacgcctgc cactcaccgc
    22861 tgtgctgatt agattccagc aagttcagat aatgaccgaa atccgttagt cccagcgaac
    22921 gcaaacgacg aaccagtcgg ttgtaaacca tgtcgcgttt atggtcagcc agaacgatcc
    22981 cggctcgttg atagatcaat tgacttatcc gccgaaaatg cgcgtcggaa agcgccaggc
    23041 gctcggtcat ctgtaacaat aaagacgttt gcccacaggg cagagatgaa gtcatagcgc
    23101 cttctcaatc acttcaggat accactggcg caatttgtaa ctgcgccgac tcatgtcgtg
    23161 ctacttcatg ttcttcaagg gtaaataccg ccacacgcga cgaaagatgg tcggcctgat
    23221 tcgccagttg ttccgttgcc accgccgctt cttctaccag cgaggcgttc tgctgagtca
    23281 cctgatccat ctggctgaca gcctgtgcaa cctgctcaat cccccgctgt tgttcttccg
    23341 acgcagaggc aatttctccc ataatgtcgt tcacgcgggt caccgaactg acaatatcga
    23401 tcatggtcgc ggcggcgtta ttcaccagtt tcgaaccctg ctggacacga ttgactgact
    23461 cttcgatcag ccctttgatc tcttttgctg cctgcgcgct acggctggca agattgcgca
    23521 cttcacctgc cactaccgca aaaccacgcc cctgctctcc ggcgcgagcc gcttccactg
    23581 ccgcattcag ggccagaata ttggtctgga aagcaattcc gtcgataacg ctgataatgt
    23641 cgccaatttt ttgcgagctg gtggcgatct cctgcatggt gtgagtcatg gtactgacct
    23701 gaacaccgcc cgcctgcgcc gttgtcgcgg cattttttgc cagttccgac gcctgtcgtg
    23761 cgttatcggc gttttgccct accgtggcgg ttaattgctc catactggcg gccgtttgtg
    23821 ccagcgatgc cgcctgctgt tcggtacgac ttgagagatc gttattgcct gcgacaatct
    23881 ccgcgatccc aatgtgcatc tcatggcttc ccttacgcac atcactcacc gtcccacgca
    23941 aagcctgctg catggtcttc aggctggcaa aaatggcggt gatctcatta cgaccatata
    24001 ccgcaatcgg acgcgccaga ttacccgcag caatgctgtc aaaatggcta ccaataatgg
    24061 ccagtggttg aacaatcatc ttgcgcgtcc accacagcgc actgctgatg tagattgccg
    24121 caacaataat catgctgata aacaccagcg ccgaaatctg atagttacgc tgactttgcg
    24181 cactggcggc ttccagcaca tggttgatct ccagctgcca ggcctcaaag ttaacgtcaa
    24241 acgcattctg tgatccctgc accggcgcag tgaggaaatc cgaaagttga ttactttcaa
    24301 gccaggtcgc ctgatgttcg agatcgttgt gccagcgggc aaaacttttc tccgtctctt
    24361 tttgcaatgc cctgacgtgc tcgttgcccg cagtcatcgc cataaaactt ttaaacagcg
    24421 tggtggattg cgtcagactg gcgcgcgccg tcgtcatcaa cgttttaatg tcatccgccg
    24481 gatagctaag tgccgtcaga gtgcccgctt tgttcagcgc ggtactggcc tgtaacatta
    24541 ccgcccgagt ttgcgctaat gccgcacgtt gctgattgct ctgctcgacc tgattcagtc
    24601 gttgcaaatc gtcgcgaaat gcccaaaaag acatgccgtt actgccaatc tgcaagatcc
    24661 cgcagagaat caaaattaaa aacagcgtgg tcgaaattcg aatacgatta aacatcaacg
    24721 ctccccatca ggcggcaatg accgcgttag taaatactcg tcaaaatgtt tcccagtttg
    24781 gatcttgttc agcaattcgc agtcgtggct gtgtcggtgg ttgctcactg gcaggacggg
    24841 atggtgtttg cggtttattg gtgagtgggc tggctgccag acggaacgtg gaaaccgctt
    24901 gcgttaaacg actcgcctgt tcttccagcg cagcggcggc ggcagctgat tcctgcacca
    24961 gcgatgcgtt ctgttgcgtg acgcgatcca tttccgaaac cgccaatgcg acttgatcga
    25021 tgccacggct ctgttcatcc gatgccgatg caatctcgcc cataatgtca gtcacgcgag
    25081 tgacagcatt gacgatattg ttcattgttt ccccggcgct ttcgaccagc accgaaccgg
    25141 tatcaacgcg tgagacggag tcttcaatga gggctttgat ctcttttgcc gcctgggcgc
    25201 tacgactggc aagattacgc acttcacccg ccaccacggc aaaaccacgg ccctgttcac
    25261 ccgcacgcgc ggcttcaacc gcggcattca gcgcgaggat attagtctgg aaggcaatac
    25321 cgtcgataac gctgataatg tcggcaattt tcttcgaact atcggcgatc tcatgcatcg
    25381 ttttcactac gccatccacc actttgccgc cgtgctgggc ggtgtcggag gcactttgcg
    25441 ccagttgcga ggcctggcgg gcgttatcgg cgttttgctt cactgtcgcg gtgagctgct
    25501 ccatgctggc ggcagtttct tccagcgcgg atgcctgctg ttcagtacgg gaggaaagat
    25561 cggtgttgcc cgccgcaatt tcacgggtac cggcatagat ggcatctgaa ccttcgcgga
    25621 catgagtgac ggtgtcagtc aaagagcgtt gcatatgtga aacgctctgc gccaggtcgc
    25681 ccatttcact gcgcccgtca atggtcaggg tattcgccag gttaccaccg gcgatttcgc
    25741 gaatgtgagc aataattttt gccagcggag taagcaacat acggcgaatg ccgtaccacg
    25801 ccaccagcag aatcaatacc accaccagcg cgataaccgc cagttgccac tgggcaaatc
    25861 ggtaatcatc tgcgttgtca gtgacgatat cgcgatacag tttttcactg ctgagggcgt
    25921 actgagcaaa cgcttcgccc attgcatttt gcattccctg ggttggctga gcgaaataag
    25981 ctccagtatt gccataatca agataatcaa tcagttcagt taacgctgtg tgatagtttt
    26041 tatatttttc atcaatatta cgactggtag cgaccatttc aggtaacggt gccatgcttt
    26101 tgaatttttt ataatgagtc gcggcctggg ccaatgtttt cctggcgcta tcgagcaatt
    26161 caactttggc gttactttgt tgattggagg aatccatcat catccgcacc gctgaacgac
    26221 tcaggttaat gcgcgtttgc agcattaaat cccaggttga cgtcagctcg ccctgctgtt
    26281 cccgtaattg attggaaacc acaaagctct tctggctatg gtgaagggaa gaaaaaaaca
    26341 gactgccgga aataagctgt aacagtgcga atacccccag caccattacc aacagcgtga
    26401 ctacgcggat acggttaatc ataaggcacc ttcctgaaaa caagttaatc tcgttatcgg
    26461 caagaagggg ggaaacttta ttgctgatgc cacccgccgc gaaattgaaa taaaaaaccc
    26521 gatgcgcaga tcatcggggt catttcaatt gaggaaatcg ggagaattac gccacttctg
    26581 acgccgcgct atctaacagc gccatctctt cgctgttcag cagtttttcg atgttcacca
    26641 gaatcaacat ccggtcgccc agtgcgccca gtccagtgag atattctgtt gaaagcgtca
    26701 cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa tgaaagcacg tctgaaacgc
    26761 cgtcaaccac gatgccgacc acccgctgtc cgagattcag gacgataact accgtgttgt
    26821 cgttatagtc cacatccacc tggctgaact taattcgtaa gtcaacaatc ggcacaataa
    26881 cgccgcgcag attcgtgacg cctttgataa acgctggcgt gttcgcaatc cgtgttacct
    26941 gatcgtagcc acggatctcc tgcactttca ggatatcaat accgtactct tcatcaccaa
    27001 gggtaaatac cagaaattcc tggcctgacg gctcgctggc cagctttgtt acattcgtca
    27061 taccggtcat attgttacct ttttactcat tcaggcggcg gtgttcgcca tacgttgttc
    27121 gcggtttatc gcctgcaagg cggagacatc aacaatcagt gccacgctgc cgtcgccaag
    27181 aatggtcgca gcagaaatgc cggggacttt gcgatagtta ctttcaaggt ttttaaccac
    27241 aacctggtgc tgaccaatta attgatccac cagcaaggcg tagcggcgac cgccactttg
    27301 taagatcacc acaattccct gggtggcttc ggttttcgcg cccgcgacgt tgaacacttt
    27361 ccacagttcg acgatgggca gatattcacc ccgcacttcc agcacccgct cgccgccggc
    27421 cagtggatgc agatcggctt cacggggttg cagtgattcc ataacagcat tcagcggcag
    27481 aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg aggatggcca gcgtcagcgg
    27541 cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac tggatttcga catgaccgcc
    27601 catctcctgg atattacgtt taacgacgtc catgccgacg ccgcgcccgg agacgtcggt
    27661 gacctgctct gccgtggaga agcctggtgc aaatatcagc atcgcgactt cgtcgtcgct
    27721 catgttttcg ctgacagtca aaccttgcga ggccgctttt gccagaattc gctcacggtt
    27781 tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg ccgccctgat gttcggcaga
    27841 cagaattaaa tttccgacgc tgtttttacc tgcggcgagc cgtttttctg gcagttcaat
    27901 accgtgatcg aggctattgc gtaccaggtg ggtcagcggg tcgataatgc gttctatcag
    27961 gcttttgtcg agttcagtag aactgcccac cagcgtcagt tctacctgct tgccgagttt
    28021 tcccgccaga tcacgcacca gccggggata gcgactaaaa acatattcca tcggcatcat
    28081 gcgaatcgac atcactgatt cctgcaaatc acgggcgtta cgttgtaact gccccatgct
    28141 ggttatcaaa tcaccatgat taaccgggtc cagttcgctg gaacgctggg caagcatgga
    28201 ctgggtgata accagctcgc cgacgaggtt aattaattga tcaacctttt ctaccgctac
    28261 acggatgctg gtggattcat tgctgcgcgt cgttttttcc cgctccacgc ggccggttgg
    28321 cgcttgttcg gctgccagtt taagcactgg tggggtggat atttttggcg agacttctac
    28381 tgtttcaaag gtaatctgat cggcttcaat cacaaaacag agtaccgctg tgatgtcatc
    28441 ttcggcgatg tcgcccggta atattgccga gagcgaatcc gcccctttca ccacgtcagt
    28501 taacgttgtc agatgtccca gttcttcttc cagcaggtcg acttccccgg ccttcaggcg
    28561 cgaaaggata attcgtcgcg gcgactgact gcgactctgc tcatcttgcg gttcactttt
    28621 ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg cctttcgctt ctaatgccag
    28681 ttgacgcaag gcctggcaga tataatcgaa gctggcggca tccggctctt gcgactgttt
    28741 ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc aaaaacagat tgataatgtc
    28801 ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc aggttttcca tcagatgcgt
    28861 ggtttcctgc aaaacgctga agccaaaagt tcctgcccct cctttgatcg agtgggcagc
    28921 ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc ggctgcaaaa ccagcagatg
    28981 ctgctccatg tcagccaaca gttcgtccgc ttcatcaaaa aatgtctgat aaaaatcgct
    29041 tatatccatg ctcacgctgt cacctcggtt cggctgatgg cattgtggga acactgacct
    29101 gtggtgcaac ctcaggtttt tccagggcgc ttactggctc attctggctt tcggcgtttt
    29161 catgcaaaat ggcctgttcg gcttgtttgt tcagtaccag caggctgatg cgacggttga
    29221 cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc cgccatgccg acgacacgta
    29281 acactttgcc gctatccaac cctccgacca tcagttcgcg gcgggatgca ttggcccgat
    29341 cggcagaaag ctcccagttg ctatatcctt tctcaccgct ggcgtagggg aaatcatcgg
    29401 tatgacctga aaggctaata cggttgggaa taccgttcag tacaggcgca atggcacgca
    29461 gaatgtcgcg catatagggt tcgacatcgg cactgccggt tctaaacatc gggcgattct
    29521 ggctatcgat gatctgaata cgtagacctt cctggaccag atcgattttg agatggggac
    29581 gtaacgcccg cagtttcgga tcggactcta tcaactggtc gagatcaccc cgcaatttcc
    29641 gcaatcgact ttgctccatg cgttttttca gctcttcgat gttcggctgc ttattcactt
    29701 ccccctggct ttgggtgtaa tcatcaccac cgccgggaat tgggctttca ctattagaaa
    29761 tgcgatcgcc gcccgtaacc gcagtcgcca gtggagtccg gaagtactcc gcaatctgaa
    29821 tcagctcttt tgggctggag atggagatca gccacatcac cagaaaaaag gccatcatcg
    29881 cagtcataaa gtcggcataa gcaatcttcc acgatccatg tgctgccccg tggcttttgg
    29941 ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt cttcatgctt cctcggttgt
    30001 cgtctgttgt tgcggatttt tcaccgcacg cacatgctct tccagttcaa taaacgacgg
    30061 acgttcgctg gaatagagcg ttttgcgacc aaactcaacg gcgataggcg gtgcgtaacc
    30121 gttcagatta gaaagcagag tgactttgac gcactgcatc attttgctgg tttcggcgct
    30181 tttctgacgt aaaacactcg ctaatgggga aataaatccg taagccaata aaatgccgag
    30241 gaaagtcccc accatcgcat gtgcgataag cgcacccagc tcggcggcag gacgatcggc
    30301 tgaacctaac gcgtgaacga cccccattac agccgcgaca ataccaaacg ccggaagtga
    30361 gtccccgacc agcgccagac tgttcgccgg gacttctgcc tcgctttcgt gcgtctcaat
    30421 ctcttcatcc atcagggctt cgatttcgaa ggtgttcata tgaccgctga taatcagacg
    30481 cagataatcg acgataaaat caagcatgac gctatccgcg aggatgcgtg ggtagctggc
    30541 gaagatctcg ctctcacggg gattttcaat atcacgttcc agcgaaaaca tccccatctg
    30601 ccgcgatttc gccatcaacc gataaagcag agccagcaga tccatataca ttgctttggt
    30661 gtatttggag cgacgaaaca gcaacggcag cgccttcagc gtgcctttaa tcgctttgcc
    30721 attattgccg acgataaacg acccaatccc tgcaccggca ataatcacca gttcagcggg
    30781 ttgatagagt gctccaaggc ttccaccggt catcaaataa ccgccgaaaa ctgtaccgag
    30841 aacaaccagg taacctaata agataagcac gacatcatcc ttccactgtt gaccatgaca
    30901 ggatgttcag tcgtcaggcg ttaacgcgcg attggggcaa aaaaaagcag cggtacgtcg
    30961 ttaccgctgc tggaatgttg cgcctcaccg tatcagttaa acagcctgta ctctctgttc
    31021 atccagcagt tgtgggataa tatcggcagg attctgggaa agtttacgtc tttttactgc
    31081 ccgggatggc ggttgacata agctgcaggc aaagctgcca acaggctggt gagcgtgggt
    31141 aataaaattg ccgccgcagc agttgcagct ggaaagttgc agtaatccac tttcaacaaa
    31201 ccgcaccaat gtccaggcac gggttaatgc cagcagtggt ccttcttctg cttgtgggca
    31261 ctgttcaagg tataaacggt aggctttgat caccgcatcg acgccattac acaaaccggt
    31321 tttcagtaaa aactgccatg cattacagaa catcgaagca tgaacgtttt gttcccaggt
    31381 cataaaccag tcggttgaga atggcagcat gcctttcggc ggtgggcttc cgcgcagttc
    31441 tttataaagt tttatcaggc gtccgcgact taactgtgtt tcgctttcca gcatctgcaa
    31501 acgagcgccc agggtgatca attccattgc cagctgaata tcccgcgctt cctgaacaat
    31561 gcttttttca ctcatgatca ggcccttttc ttgcgcagcg cttcttcagg ctgattaaca
    31621 tcattcagca agcgtgttga gagcatgatg ccggtatgaa tttgctggag atcgtcaacg
    31681 cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt caaaacggaa gtgacaaacc
    31741 agttgattgg tttctgccag cttaaccatt tgcggaagag tcagtgccgc taacgttgtc
    31801 gccatttctt catttatgcc gagacgaaac atagcggacg ctttgtcctg aacaatcaaa
    31861 cgctgtgcaa gtagtaaata tgacaagttg atgtcataaa tgtgtttcag caactcggag
    31921 gtatgcatta ttcccaccca gaataaccaa ctttattttt atgcggtttc accgcacccc
    31981 gtgatgtcgc cgggaagccc cggtaaaaaa taattagcat tagaatagtt gcgataagct
    32041 gcaataagca gaaccacctt tttggtttaa tatgtcctta caaatagaaa tgggtcttta
    32101 cacttatcta agatttttcc taaatcgacg caactgtact cgtcactaca cgcacataca
    32161 acggaggggg gctgcgattt tcaataatgt gtgatgcaga tcacacaaaa cactcaatta
    32221 cttaacataa atgtataagt catacttttg ttttgggtgt atttcaatct gttaaaaagt
    32281 ttttcgctac gctagcaagc aaaaatgaaa caggaataat cgaaatggga tgttgcgcac
    32341 acttaaaata actcaccgta aataatcatc tgctataaat aatcactttc atgcaatacc
    32401 agataagcta tttttaaaca gacacttacc gcacaacaaa ctaattaaat acaaagaaag
    32461 gcaacaatca acttaatgtt aatgaaatgt tttacatata ttaaccactt atttaatgcg
    32521 gagaatcagt acactatttt cttataatta catttgaaat tatatgatca ccgggagtga
    32581 ttgattaatt gtctttacta ataatcagac taatatttac ctgtttgacc gagttgggat
    32641 tgcgtcgttt ctccattagg agtaaagctt taatgtcacc tgaagttcac agaataagga
    32701 acaggttatg agctatagca atattcttgt cgctgttgcg gttacaccgg aaagtcagca
    32761 actgctggca aaagcggtat ctatcgccag gccagtaaag ggacacatca gtttaattac
    32821 tctcgcttcc gacccggaaa tgtacaatca attagccgcg ccgatgcttg aagatttacg
    32881 cagcgtgatg caagaagaaa cgcaaagctt tcttgataag ttaattcagg atgcggggta
    32941 tcccgttgac aagacgttta ttgcctacgg tgagttaagc gaacatattc tggaagtatg
    33001 tcacaaacat catttcgatt tggtgatttg cggtaatcac aatcacagtt tcttttcgcg
    33061 agcatcctgc tcggcgaaaa gagttattgc ctcaagtgag gtcgatgtgc tgttagttcc
    33121 acttacggga gattaaccgc tcctacgcaa gctttggaaa ggtagcaact ttatcgcgct
    33181 gctggctttc cgcgcttcgc ggaactatct gctttaggtc gctaatgaag cactcctgcc
    33241 agtggttaat atcgtttttc acgataacgt ccagcatttc tgcatgacgg gaaatacgtt
    33301 ccgccagcga catagtcaat gcacgatcca gcgcagctgc aacttcgtca cgatcgtagg
    33361 ggttaacaat taacgccgac gttaactcgt ttgccgctcc cgcaaattgc gaaagaacaa
    33421 gaacgcccgg gttggctggg tcctgagcag caacatactc ttttgctacc aggttcatcc
    33481 cgtcacgcag tggcgtcact aagcccacgt cagagtagcg gaatattttc atcagtaatt
    33541 tacggtcaaa atgctgattc aaataataaa gcggcgtcca gcctaattgc ccgtatttac
    33601 cattaattcg tccagcttca ttttcgagct gatgacgaat atcctgatag gcttgcacat
    33661 caccacgcga cgttggtgca atctgggtat aacgaatttt accatgatgc tgcggatatt
    33721 tttccagcaa cgcttcatag gcgagaaaac gctctggcaa acctttggaa taatccagcc
    33781 gttcgacaga aaagatattt tgtacgtttt tcagttccgc tttaagttgc gccagttttg
    33841 gcggcagtgg cccggcagcc tgtttggcta tttctttcgg ttcaatgccg atcgggtaga
    33901 cttctgttcg aaatgctttg ccccaggctg tatggctttt tgcgctacgt gtcgtgacgc
    33961 gggtcaggtt agaaagacaa tccaggaacg ccagacgatc gttttctgtc tggaaaccca
    34021 gcaaatcata atcacaaagc tgttcaagca aggtgtcata tgtcggcagc gcgttgaaga
    34081 tttccggtgt cgggaaagga atatgcagaa agaaaccaat gcgattattc actccccgtt
    34141 tgcgtaattc atgcgcaaat ggcaacaggt gataatcgtg gatccagata atgtcatcgt
    34201 cttgcaacag cggcagtaat ttatctgcca gcaacgcatt tacgcgtaga tagccgtccc
    34261 aggcaggacg ctgaaattgc accagatcga gccgataatg aaaagcgggc cagagaacgg
    34321 cattggagaa ttggttgtag tattcgtcaa ggtcctgttc gctgaggtta aaagaggccc
    34381 acgtaatgtt accttttttc acctttttta gcggctgatc ctcattccct gtttcaccac
    34441 tccagccaaa ccacagtccg cctgcggctt tcagtgcccc cagtatgcca acggcaaggc
    34501 caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg gttagatact acgactaaac
    34561 gactcatagt catcactcct gttattttct cttttttgtt gtaatgcggt ggttatcatt
    34621 tcaagccagc tccagacatc cggcacaccc gccagtcgcc atgatgcctg agttgcacct
    34681 gtgccaattt ttactgacat tccgcccagt cggttaacga ctgcgaagcc agattcatcg
    34741 gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa agggagcttc ctgcataaaa
    34801 gctgcaattg cctcaccttt actggtacct ctcggtttga tctcgacaac acactttccc
    34861 tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt gcgctaatgt cattaatgcg
    34921 tcttcatgct gcggagcctg acgataatgc agcgcaaaag ccatcccttt cgcctccagc
    34981 tccgcgccgg gatactgagc gatgactgta tgcagttgca cgctaatatc acgcgcaatc
    35041 gcatccggca gatgaacgat atgtgtttta ccattgatgt cacggcgctc cgccccatgc
    35101 acgcccgcta acgggaagcg ataaggtttt gccagtgcgt caagctccac cattgagcgc
    35161 cctgatatca atgccaatgc accatcactt gcggttgcca gtagctgtag tccttgcaga
    35221 atattgtcag gcacgacgac ctgatcggga tgcggtttga tttccgccag cgttccatca
    35281 agatcaaaaa accaggcata tttcgcggat agttcagggg tttcggttaa cggttctgtc
    35341 acccggctct cctccttctc ttttcttatt gttttgcgca gaacgcgctt tacagacatg
    35401 taagtataga cagtgtgacg ggggtcgcca tttgcaatga agaacagcca accgttgtgg
    35461 cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga aaaaagcatc agacagtgcg
    35521 tttcgctttt tgcttgtaac ggtcgaagat caccgctgcc agcaggatta agccgcgaac
    35581 cacgtactgc gcgaaaggag aaatattaag caggttcatg gcgttttcca cggtgcctaa
    35641 aattaagata cccgccacca catatgagat ttttccgatg ccacctttca gagaaacgcc
    35701 acctaaaacg caggcggaga taacaataag ctcataacca atcgacgtca ttggctgccc
    35761 actagtcata cgtgaagcca gaataattcc ggctatcgca gataccaggc cagagagaac
    35821 aaagataata attttggtgc gaacaaccgg tacacccgcc agacgcgcag cctcttcgtt
    35881 cccgccaatc gccagggtgt tacgaccaaa ggtggtttta ttcagcaaca aaccaaagat
    35941 aatcagacac gcgacggtga gccagattgg cgcaggcaga ccgaaccagt tagcgtaacc
    36001 aagggcaaag aagctttcat cttcgatacc gaccgcttta ccgtctgaaa tgatgtacgc
    36061 cagacctcga acaatctgca tcgttgccag tgttgtgatc agagcattta ttttcagttt
    36121 ggcgataaca aagccattga ccaggccaca gagaatgcca agcagcaacc ccgctgccac
    36181 gccaatccac aggctttcag tcaggttgat aaccaccgcc gtggtgacac ccgcacaggc
    36241 aattacggag gcgacagaaa ggtcaaagtc accggaagca aggcagaaca acatgccaca
    36301 cgccaccatc cccgacatgg aaattgccag gcccaaccct ttcatattaa tgaaggtggc
    36361 aaaatttggg acaaaaatgg cacaggcgat aaagagcacc gcaaacacca ccagcatgcc
    36421 gtactgatcc cagatacgcc cgaagctgaa tgacgactta ggtgcgccag accccgatgt
    36481 agaaacagaa gacatcatac tctccttact caggcaactg cctggctgac tttaggcatc
    36541 gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta acaattcacc ggcgatttca
    36601 ccttcccgca tcaccacaat ccggtcggca acgccgagga cttcaggtaa gtcgctggag
    36661 gcaaacagca ccgccacacc ctgcgccgcc agcgcataaa tcacgttgta aatttcatgc
    36721 ttagcaccaa catcaatgcc gcgcgtaggt tcatccagca aaatgacctt catctcttcc
    36781 gataaccagc ggcccagaat ggctttttgc tgatttccgc ctgagagatt catgatcagt
    36841 tgctcagcgc ccggcgtttt gatgttgagc gaacgaatgt ggtgatcggc attgttttct
    36901 tcccaaccgt tgttgattac acaaccgccg agcacatgtt tacgtctggc actgatgttg
    36961 atattgtcgc gaacggagtg cacgggaata atgccttccg ctttgcgatc ttccgggcag
    37021 agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa tatcgatcgg ctgttggtcg
    37081 atataaacct gacctgcggt gatttgcgtc ccgccaaaca agcctttcat taattcgcta
    37141 cgccccgccc ctaccagacc aaacagccca acaatttcac cactgcgaac cgccagactt
    37201 attggcgtac gcacgcctgg tgctttcaca gcatcaagac gcaggcgctc ctcgccataa
    37261 ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc cgaccatcgc ctgcaccagc
    37321 gcgtcgtggt caacctgctg catatcggta aaggttttga cataacgtcc atctttaaag
    37381 acagtgatgg catcgctgag ggcaaatatt tcttccatac ggtgagaaac gtataagatc
    37441 acccgcccct cttttcgcag ttcacgaata acgcggaaaa gattgtcgat ttcgcgggca
    37501 gaaagggagc tggttggctc atcaaaggcg ataattttag cgttacgcgc cagcgccttg
    37561 gcgatttcaa ccatctgcca ctgaccaatg gagagatatt tcagcggcgt gtccgggtca
    37621 atatccatac caagatgttt aagttgtaaa cccgcctcat aattcagcaa tgagcgattc
    37681 acaatgccgc ctttatgcgg cagctggccg agatagatgt tttccgcgac ggtcatttcc
    37741 ggcacgagat gtagttcctg gtaaataatc gccacacccg cgttaagtgc tgcggtcgtg
    37801 tcggaaaagg acatttcctg cccattaatc actacagaac ccgtggttgg cgcatagttg
    37861 ccgctgagga tttttaagag agttgatttt cctgcgccat tttcacccat caacgcatga
    37921 acctgaccgg catagcagtc aaaactaata tccgtcagcg ccttaacgcc gggaaacgtt
    37981 ttaccgatgc cgcgaaatga gagatacggg gtagactgtt gcataacgtc tccgtgaatc
    38041 actggtcgtt cacaccccct ctgcatcatg cagaggggaa tttttccggc aaattactta
    38101 ccgcctaaac cttttttctc cagttcttct ttaaagttgt cacgcgtgat cagtaccacg
    38161 tcggtaactt cggtaaattt tggcggttca acgtcttttg ctacccagtt gtaaagcatt
    38221 tcgctggatt tatagccatg tacgtccggg cttggcagca gggaaccgta gaagccggtt
    38281 gcctgtgctt tagacagttc gctcaccgca tccacaccgt taatgccaat gccgatgata
    38341 tcggccgctt taaagccctg accttccgtc gcgcgtacgc cgcccagcac ggtgctgtcg
    38401 ttcataccga cgatcagcca atgtttaact tccggatgtt gaaccagcat tgagttggca
    38461 gcgtcaaatg cccccgggat gtcgttagat ttggtaggta cctgataaat ttgtttttcc
    38521 gggaatccgg ccgctttcag cgcatccata gatcccgtag tacggcggcg ggcggtatcc
    38581 agttcgttgg cggtaatctc catcaccgcg ctttctttga catcccagcc acgtttctgc
    38641 atctctttat acagttcctg gccctgacgt tcgccaattt tagtcgccgc catcatcacc
    38701 agcggaacgg tatccattgg cttacctttg gcgttaacaa actggtcatc cacggcaatg
    38761 acttccatat cgtagccacg cgctttcgcg acgatggcag agccgagttt ggggtccgga
    38821 gtacaaataa cgaaaccttt tgcgccactg gcagccaggc tgtcgatcgc gttcaatgtt
    38881 ttttcgccat ccggcacggc aatcttaata acctcaaacc ctaaatcctt cccggcttta
    38941 tcggcaaact tccattcggt ctggaaccac ggctcttccg gttgcttcac cagaaaaccg
    39001 agcttcaggt tctccgccat agcggattgt gacataacgg ctgccagacc aatggctgcc
    39061 agggctttag taaatttgtg catggttctc tccagcttta gtgtcgtttt gtgtagggca
    39121 aaaacgaatg acattcgtta aattaatcgg aaaacaaagc attacctttt aactaaaaga
    39181 taagtgactg tgttgacata gttttagcga gaaattaatt ctccatagga gagcaatatc
    39241 acatcgcaga attacagtga gaacgtgcat aaatttagcg ggaaaagaca taagggaaag
    39301 ccaatttgtc agacaaattg tcgaatgcac agcagattaa tccataagat tagcctggaa
    39361 atccttgttg tctttggtac ccatgcggga tgtcttcttt ttaaccagtc aataggccgc
    39421 attacctggc gttgagtttt tgaaatggtg taataaccgc aactcaaaga tgtggaaaat
    39481 gcacgtcatt catttcgcca ttaattatca ctgtgctcat taattaacag aacacgtata
    39541 atgagagcca tctcgcaaaa atgaaaaaac gttttataaa atcatcactt catcatgaat
    39601 tcaaattcat tgattaatat caacaagata caaaaagcgc tatcattaaa attcattgca
    39661 gttacattga tttcatcaat gaaatgtaaa aatatataaa cttgatgatt taagcatttt
    39721 ctaatacccg ttcagacgtt attcttattt cagatcatcg tcagaattga ctccacgatc
    39781 acatttcgga ccggcagaaa ggaattattc tgcaaacagt aattatggtg ttttgattta
    39841 tcttgcacct ctccacttct ggatataagg atattaggta tggcaaccgc tggaatgctt
    39901 ctcaaactca actctcaaat gaaccgcgag ttttacgcat ccaatctcta ccttcacctg
    39961 agtaactggt gttctgaaca gagtctgaac ggcaccgcca ctttccttcg cgcccaggca
    40021 cagagtaatg tgacccaaat gatgcgcatg tttaacttta tgaagagtgt cggcgctacc
    40081 cccatcgtta aagccattga tgttcccggt gaaaaactga actctctgga agaactgttc
    40141 caaaaaacga tggaagaata cgagcaacgt tctagtacgt tggcacagtt agccgatgaa
    40201 gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag
    40261 cagcatgatg gtctgttgct gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg
    40321 ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat
    40381 tgatcatcat cggcgctaat gcattgcgcc gatgaaggtt ttgagaaacc gctgcctcat
    40441 ctgtttgaag cagcggtttt tttaatggga ttcaccctgt ggggtaaact tgagttcaat
    40501 aagcgcgatg gctttttgga ttgcccgcat ggtgaccggg tctgcggcgg cgggatggtt
    40561 agtaaagtcg atattcttca gctgactgga cattttttca cgaacttcaa cgggcgcgat
    40621 tacatcgaga acatccagaa tttgtttgat aaccaactgg caagcaacca catcagaaac
    40681 cagttcctga tcggcattca gcggctggga catcgtaaac tcctgatagc attttgaaag
    40741 ccgttatagt agcggcttca catcttcagc gatagtcaca tccaccgtca tcaggacaca
    40801 aaaaacctgc cggagcaggt tttttattat cggaacatat tgcctggcgg tacgtctttg
    40861 aacgtcttgc aatagttatt gaacatactt ttcaggattt tgcgcagttt catcgcggca
    40921 ctccgaccat ttgttataca ggtgttattc tctttgcgct tataatatga caaccatcac
    40981 aaaaatcaat ctttatgtga tacaaatcac ataaataccc ctttaatgtt ataaaaatga
    41041 taatcaaaaa acagcccccc tatttctgac acctacagat ggcaagaaat agcgcctgcc
    41101 aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac
    41161 tcttctggct ggaccatgag acttctgatt ctgactcttt cattaataac gctggcgggg
    41221 tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg cggcaacagg tattgtacgg
    41281 ttggtttatg accaggcttt tttgcagcat gcccataccg atcgttatgt cagtcgcggg
    41341 atcgccgatc gcgcatgcca gcaggaaggc tatacccacg cggtcccctt tggtcagcca
    41401 gtaggcaact gtagcctttt tgcgggttcc ttatgtttga atacggaatt cactttgtca
    41461 tatcagtgcc atcattccgc cttccctgtc tttctctaaa tattttaatc agcgagggga
    41521 tcttcgctga ttaaagaaat agcggtaatg cgttttattc ccattcgtat ttttaataat
    41581 tgaagtttat attttacctt ttgcaaataa taaaataaca aattatagtg acgccacagc
    41641 aacaaatata acctttgtgg agcactatca tgctgaaacc agaaatgatt gaaaaactta
    41701 atgagcagat gaacctggaa ctgtactctt cactgcttta tcagcaaatg agcgcctggt
    41761 gcagctatca taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga
    41821 tgacgcatat gcagcgtctg tttgattacc tgactgatac cggcaattta ccgcgtatta
    41881 ataccgttga atctccgttt gctgaatatt cctcacttga tgaattattc caggaaacct
    41941 ataaacacga acaattaatc acgcagaaaa ttaacgaact ggctcatgct gcaatgacca
    42001 atcaggacta cccaacattt aatttcctgc aatggtatgt ttctgagcag catgaagaag
    42061 agaaactgtt caaatcgatt attgataaat taagcctggc aggcaaaagc ggcgaaggtc
    42121 tgtattttat cgacaaagaa ctctctaccc tcgacacaca aaactaatgc tctcaggcgg
    42181 caggttttct gccgccgttt caaatcagtg acggcaaatc ttactctgat cggtcgaaaa
    42241 accttcttct gaaggtataa atttgccttt tgctgccaga aacgctacca gctctcccgc
    42301 agtcatccct tctgccgagc aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat
    42361 tgctgcttcc agactggcat gcgtatattg ctcgcctgat tcaatcatca tatttaacac
    42421 ttcatgaccg tgaatagagt ccatcgtccc tcctcaaaaa aagcctagcg tagcgattgc
    42481 cgcttatgaa gactttgcgc cagcgcagga ctgaatgctt tttattgtac atttatattt
    42541 acaccatatg taacgtcggt ttgacgaagc agccgttatg ccttaacctg cgccgcagat
    42601 atcactcata aagatcgtca ggacagaaga aagcgtgaaa aacagaaccc tgggaagtgt
    42661 ttttatcgtg gcgggaacca caattggcgc aggcatgctg gcaatgccgc tggctgcggc
    42721 cggtgttggt tttagcgtta cgttaatctt gttgattggg ctttgggcgt tgatgtgcta
    42781 cacggcgcta ttactgctgg aggtgtacca gcatgttccg gcagataccg gtcttggcac
    42841 gctggcaaaa cgctatctgg gacgctacgg tcaatggctg acgggcttca gtatgatgtt
    42901 cttaatgtat gctctgactg cggcatacat cagcggtgcc ggtgaattgt tggcctccag
    42961 catcagcgac tggacaggta tttcaatgtc ggcaaccgct ggcgtgctgt tgttcacttt
    43021 tgttgccggt ggcgtggttt gtgttggaac atcgctggtc gatttattta accgttttct
    43081 gttcagcgcc aaaattattt ttctggtggt gatgctggta ttgctgctac cgcatattca
    43141 caaagtgaat cttttaaccc tgccgttgca acaggggctg gctctgtcag caatcccggt
    43201 gatttttacc tcatttggtt ttcacggtag cgtgccgagt attgtcagct atatggatgg
    43261 caacgttcgt aagctacgct gggtgtttat aaccggtagt gcgatccccc tggtggcata
    43321 tattttctgg caggtggcaa cgcttggcag cattgattca acaaccttta tgggattgct
    43381 ggctaatcat gctggattaa acgggctgtt acaggcgtta cgcgaaatgg tggcctctcc
    43441 gcatgttgag ctggcagtgc atttatttgc tgatttagcc ctcgccacgt catttctcgg
    43501 cgttgcgtta ggcttatttg attatctggc tgatttattt cagcgttcaa ataccgttgg
    43561 tggacggttg caaactggtg caattacctt tctgccgccg ttggcgtttg cactgtttta
    43621 tccacgagga tttgtgatgg cgctgggtta cgccggtgtg gcgctggcgg tactggcatt
    43681 gattatccct tcgctgttga cctggcaaag cagaaagcac aatcctcagg cgggttaccg
    43741 ggtgaaaggt ggtcgtccgg cgctggtggt ggtgtttctc tgtggtattg ctgtgattgg
    43801 cgtgcaattt ttgattgcgg cagggttgtt accagaagtg gggtgatcag atagcctcaa
    43861 attccttatt gggtgccaga attaacgctg acacccaatt tgacctctta atgcaggcag
    43921 cactgcttaa atttcttacc actaccgcac gggcaaggat cgttacgccc cggtttctct
    43981 tctgctttga tcggttgctg aacagctttt tcctgcggat gcgccatcca gtacgcatgt
    44041 agatcaagcg ccgccagtcg aatggcatct acgctctctt caaacgcttc tggcgacatt
    44101 ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg caatcgcctc cagcgctggt
    44161 tttaacgaat cgggcaacgt tgaccagtca gaaagtgcca cgccccgcat atagccaaag
    44221 caccactcct caacaatcgt cagctcgctg ccatcaactt ctcgcaagcc gaataacggc
    44281 tcaaactgct ccgggaattc gttcagacgc tctgcggtat cggccatatg ttgaaaagcc
    44341 agattcataa agcgcgtcat ctctttctct gacgcccagc gcggcacata gtcagcccca
    44401 ccccacacgg caaccagcca ctgttccggt tcaatctctt gcggagaact caacaccgcc
    44461 gtcaataaac cgtccagctc cgccacatca aggatggcgt ggtcagtgtt gtatttggtc
    44521 agaatatcgt ccagccattc caactcactt tcgtttaacg gtcccgtttt catacgcttt
    44581 tccttgtgga tctcaactcg ccagcaccta tcttacatgc cggtccgtat cagagatact
    44641 ttttgagtgg ctttgctggt gattaaaaat tagggagggt gtaacgacaa gttgcaggca
    44701 caaaaaaacc
//
LOCUS       DAZFYC010000026        44510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFYC010000026 DAZFYC010000000
VERSION     DAZFYC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.026"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..493
                     /gene="tnpA"
                     /locus_tag="ACHKW5_001631"
     CDS             62..493
                     /gene="tnpA"
                     /locus_tag="ACHKW5_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO7511043.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(509..697)
                     /locus_tag="ACHKW5_001632"
     CDS             complement(509..697)
                     /locus_tag="ACHKW5_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO7511044.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(701..1060)
                     /gene="yeaR"
                     /locus_tag="ACHKW5_001633"
     CDS             complement(701..1060)
                     /gene="yeaR"
                     /locus_tag="ACHKW5_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO7511045.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1231..1869)
                     /gene="leuE"
                     /locus_tag="ACHKW5_001634"
     CDS             complement(1231..1869)
                     /gene="leuE"
                     /locus_tag="ACHKW5_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO7511046.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIIRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLTKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1996..2919)
                     /gene="dmlR"
                     /locus_tag="ACHKW5_001635"
     CDS             complement(1996..2919)
                     /gene="dmlR"
                     /locus_tag="ACHKW5_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO7511047.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDAGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYQNFSLEHA"
     gene            3022..4107
                     /gene="dmlA"
                     /locus_tag="ACHKW5_001636"
     CDS             3022..4107
                     /gene="dmlA"
                     /locus_tag="ACHKW5_001636"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO7511048.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4358..5968
                     /gene="yeaV"
                     /locus_tag="ACHKW5_001637"
     CDS             4358..5968
                     /gene="yeaV"
                     /locus_tag="ACHKW5_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO7511049.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFLTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6000..7124
                     /gene="yeaW"
                     /locus_tag="ACHKW5_001638"
     CDS             6000..7124
                     /gene="yeaW"
                     /locus_tag="ACHKW5_001638"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO7511050.1"
                     /translation="MSNLSPDFVLPENYCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7180..8145
                     /gene="yeaX"
                     /locus_tag="ACHKW5_001639"
     CDS             7180..8145
                     /gene="yeaX"
                     /locus_tag="ACHKW5_001639"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO7511051.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8199..9314)
                     /gene="rnd"
                     /locus_tag="ACHKW5_001640"
     CDS             complement(8199..9314)
                     /gene="rnd"
                     /locus_tag="ACHKW5_001640"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO7511052.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9396..11081)
                     /gene="fadD"
                     /locus_tag="ACHKW5_001641"
     CDS             complement(9396..11081)
                     /gene="fadD"
                     /locus_tag="ACHKW5_001641"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO7511053.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11286..11867)
                     /gene="yeaY"
                     /locus_tag="ACHKW5_001642"
     CDS             complement(11286..11867)
                     /gene="yeaY"
                     /locus_tag="ACHKW5_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO7511054.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11907..12602)
                     /gene="tsaB"
                     /locus_tag="ACHKW5_001643"
     CDS             complement(11907..12602)
                     /gene="tsaB"
                     /locus_tag="ACHKW5_001643"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO7511055.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12660..14570)
                     /gene="yoaA"
                     /locus_tag="ACHKW5_001644"
     CDS             complement(12660..14570)
                     /gene="yoaA"
                     /locus_tag="ACHKW5_001644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO7511056.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLHSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14702..15046
                     /gene="yoaB"
                     /locus_tag="ACHKW5_001645"
     CDS             14702..15046
                     /gene="yoaB"
                     /locus_tag="ACHKW5_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7511057.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQTGLMNP
                     KYKVEIKIVAAV"
     gene            15468..15767
                     /gene="yoaC"
                     /locus_tag="ACHKW5_001646"
     CDS             15468..15767
                     /gene="yoaC"
                     /locus_tag="ACHKW5_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO7511058.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(15887..16066)
                     /gene="yoaH"
                     /locus_tag="ACHKW5_001647"
     CDS             complement(15887..16066)
                     /gene="yoaH"
                     /locus_tag="ACHKW5_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO7511059.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16140..17501
                     /gene="pabB"
                     /locus_tag="ACHKW5_001648"
     CDS             16140..17501
                     /gene="pabB"
                     /locus_tag="ACHKW5_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO7511060.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            17505..18083
                     /gene="nudL"
                     /locus_tag="ACHKW5_001649"
     CDS             17505..18083
                     /gene="nudL"
                     /locus_tag="ACHKW5_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO7511061.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18267..19631
                     /gene="sdaA"
                     /locus_tag="ACHKW5_001650"
     CDS             18267..19631
                     /gene="sdaA"
                     /locus_tag="ACHKW5_001650"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7511062.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPLKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            19762..21360
                     /gene="pdeD"
                     /locus_tag="ACHKW5_001651"
     CDS             19762..21360
                     /gene="pdeD"
                     /locus_tag="ACHKW5_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7511063.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(21364..22920)
                     /gene="yoaE"
                     /locus_tag="ACHKW5_001652"
     CDS             complement(21364..22920)
                     /gene="yoaE"
                     /locus_tag="ACHKW5_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO7511064.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(22908..23066)
                     /gene="yoaL"
                     /locus_tag="ACHKW5_001653"
     CDS             complement(22908..23066)
                     /gene="yoaL"
                     /locus_tag="ACHKW5_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO7511065.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            23383..24354
                     /gene="manX"
                     /locus_tag="ACHKW5_001654"
     CDS             23383..24354
                     /gene="manX"
                     /locus_tag="ACHKW5_001654"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO7511066.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            24417..25217
                     /gene="manY"
                     /locus_tag="ACHKW5_001655"
     CDS             24417..25217
                     /gene="manY"
                     /locus_tag="ACHKW5_001655"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO7511067.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25230..26081
                     /gene="manZ"
                     /locus_tag="ACHKW5_001656"
     CDS             25230..26081
                     /gene="manZ"
                     /locus_tag="ACHKW5_001656"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO7511068.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26136..26594
                     /gene="yobD"
                     /locus_tag="ACHKW5_001657"
     CDS             26136..26594
                     /gene="yobD"
                     /locus_tag="ACHKW5_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO7511069.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            27023..27589
                     /gene="mntP"
                     /locus_tag="ACHKW5_001658"
     CDS             27023..27589
                     /gene="mntP"
                     /locus_tag="ACHKW5_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO7511070.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(27586..28395)
                     /gene="rlmA"
                     /locus_tag="ACHKW5_001659"
     CDS             complement(27586..28395)
                     /gene="rlmA"
                     /locus_tag="ACHKW5_001659"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO7511071.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(28561..28770)
                     /gene="cspE"
                     /locus_tag="ACHKW5_001660"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(28561..28770)
                     /gene="cspE"
                     /locus_tag="ACHKW5_001660"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7511072.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(28783..28926)
                     /gene="yobF"
                     /locus_tag="ACHKW5_001661"
     CDS             complement(28783..28926)
                     /gene="yobF"
                     /locus_tag="ACHKW5_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO7511073.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(29596..29883)
                     /gene="yebO"
                     /locus_tag="ACHKW5_001662"
     CDS             complement(29596..29883)
                     /gene="yebO"
                     /locus_tag="ACHKW5_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO7511074.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(29958..30101)
                     /gene="mgrB"
                     /locus_tag="ACHKW5_001663"
     CDS             complement(29958..30101)
                     /gene="mgrB"
                     /locus_tag="ACHKW5_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO7511075.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            30260..30499
                     /gene="yobH"
                     /locus_tag="ACHKW5_001664"
     CDS             30260..30499
                     /gene="yobH"
                     /locus_tag="ACHKW5_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO7511076.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(30642..31433)
                     /gene="kdgR"
                     /locus_tag="ACHKW5_001665"
     CDS             complement(30642..31433)
                     /gene="kdgR"
                     /locus_tag="ACHKW5_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO7511077.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            31610..32983
                     /gene="yebQ"
                     /locus_tag="ACHKW5_001666"
     CDS             31610..32983
                     /gene="yebQ"
                     /locus_tag="ACHKW5_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7511078.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(33029..33910)
                     /gene="htpX"
                     /locus_tag="ACHKW5_001667"
     CDS             complement(33029..33910)
                     /gene="htpX"
                     /locus_tag="ACHKW5_001667"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO7511079.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(34102..36150)
                     /gene="prc"
                     /locus_tag="ACHKW5_001668"
     CDS             complement(34102..36150)
                     /gene="prc"
                     /locus_tag="ACHKW5_001668"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO7511080.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(36170..36868)
                     /gene="proQ"
                     /locus_tag="ACHKW5_001669"
     CDS             complement(36170..36868)
                     /gene="proQ"
                     /locus_tag="ACHKW5_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO7511081.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(36965..37462)
                     /gene="msrC"
                     /locus_tag="ACHKW5_001670"
     CDS             complement(36965..37462)
                     /gene="msrC"
                     /locus_tag="ACHKW5_001670"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO7511082.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            37592..38875
                     /gene="yebS"
                     /locus_tag="ACHKW5_001671"
     CDS             37592..38875
                     /gene="yebS"
                     /locus_tag="ACHKW5_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO7511083.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            38844..41477
                     /gene="yebT"
                     /locus_tag="ACHKW5_001672"
     CDS             38844..41477
                     /gene="yebT"
                     /locus_tag="ACHKW5_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO7511084.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            41557..42996
                     /gene="rsmF"
                     /locus_tag="ACHKW5_001673"
     CDS             41557..42996
                     /gene="rsmF"
                     /locus_tag="ACHKW5_001673"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO7511085.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            43114..43350
                     /gene="yebV"
                     /locus_tag="ACHKW5_001674"
     CDS             43114..43350
                     /gene="yebV"
                     /locus_tag="ACHKW5_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO7511086.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            43455..43646
                     /gene="yebW"
                     /locus_tag="ACHKW5_001675"
     CDS             43455..43646
                     /gene="yebW"
                     /locus_tag="ACHKW5_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO7511087.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(43647..44303)
                     /gene="pphA"
                     /locus_tag="ACHKW5_001676"
     CDS             complement(43647..44303)
                     /gene="pphA"
                     /locus_tag="ACHKW5_001676"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7511088.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
BASE COUNT        10865 a        11397 c        10910 g        11338 t
ORIGIN      
        1 ctcctgttga tcacctggtc ttaactggaa tttaaatgcc tgtagtcgtt tcacaggcca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac cagatacaga cgtcagattt ttgaccatga
      181 cgcgacagaa aaactacgcg cttacttttc aaatgtatgt gctgattttg aagctgaact
      241 tgttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactatc ctcccaaact
      301 ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac tgcgacgaga
      361 tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc ctggctattt
      421 tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg aacaacagca
      481 aacacctggt taggtggaaa accgcgcctt atatccccgc cctgaaggac ggggttttac
      541 ggcgcaccgg ataagaacgc acggtattca ccagatcttt tatcacttca gccgccactt
      601 ctggcaccag caaagtcatc ggcgtctctg tttcataatc gacagaaacg ccattgctgt
      661 tattggtgac ggtcacggta tacgttgctt tgcccatgat tcatttcccg ttatgaatga
      721 ctttccgttg ttgcgcacct tccatcagga cttcaggagc cacgaagaag tcaatgttga
      781 aataagtatc gtcagtcatg gcttcaatgt tgtgccactt ttctggaggg aacaccgcaa
      841 actgccccgc ttcgataagg atcacctgat caggctctgc actgtgttca tcagcgtagc
      901 cgagatattt gaccgcccca tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc
      961 ctttatcaag atgacgttcg aatattccgg caggcgcagt ttgtttattc cagaaaggcg
     1021 ttgagcgcgt atgaatataa ttctgtggga tttgcagcat ctttttccct ccttcgttga
     1081 atgcgctgaa aacggtattc cagccgtttc agagtacgcc tgataatttg cattttaaat
     1141 accatttatt ggttattttt tagcatcata tcagagaaga atcagggagg attacaagtg
     1201 ggaagcccgc gacaacgcgg gctgaatgta tcaggattgc agcgtcgcca gtcgggcagc
     1261 gaaacccacg aacatcaaac caatcagtga gttgcccact ttagtcagtt tctttttggt
     1321 acgtatgtac tgcgtgacaa aagcaccaga aataatcagg aagctcaaat agcagaaact
     1381 caccagttcc agcgtcgtcg ccagaataaa gaatgaaatt cccgtatgtg gggcattaac
     1441 atcgataaac tgtacgaaaa acgacacata gaacaaaatg gctttcggat tagtcaggct
     1501 caaaattaac gcgcgtttaa aaatagcacc gtattggggc tcatcggatt tggcctcgtt
     1561 atttttaccc ttcagggtcg cgtagagaat tttactcccc agatagagca aataaaacgc
     1621 accaagataa cgtataatgt tgaataatat cggggtggtc ttaattaatg tcgccactcc
     1681 agcccatgcc agaaacatca ataccgcatc gccaataaat acaccgcagg ccgcaagata
     1741 accgcctttc ataccgctac tgacgctatt tttgagtaca aacagggtat ttggccctgg
     1801 caccaacaca ataaaaatgg ccccaaccag ataggtccag taattcagaa ccccgtattc
     1861 agcgaacaca ttaacctctt taattatctt tcgatcatgc gcgattaaag gtgaatatgc
     1921 taaccaatct gtagcggctt agaaaggaga aaatcaggtt ttaacccgat atcaacccga
     1981 taattgaata attaatcagg catgctccag tgaaaaattc tggtagtgct cggcaaaata
     2041 ctggcgtaaa aattctaccg ttatccgcac tttcgctgac gtcgccagcc ttgaaacata
     2101 aacggcccag acgttcgctg gctggtaata ttccggtagc acttgcacta aatgaccact
     2161 ggcaatgttt tcgctaacat cccaccagga acgcagagca atcccctgcc catcgagaca
     2221 ccactgatgg acaatttctc catgattaga cgacagtggg ccggtgactt taatcgcatg
     2281 gtggccttct ttattgcgta attgccagac accaaaagga tgatcgcgct ctttaatgac
     2341 caagcacggc aatgcagaaa gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa
     2401 ttccggcgac gcgcagagaa tgcggtaatt ggtcgccagt ttgcgggcga ttaaattagg
     2461 ggcgatgtca tcgccaatgc gaatatcgag atcgacacct tcattgacca aatcgaccag
     2521 tcggtcttcc acatcaaaac gtaattccag ttgcggatac gccttcgcca gcgctgataa
     2581 cgccggagcc accacctgtc gtccaaaacc aaagctgctg ataatacgca gcatcccctg
     2641 gggcacctga cgcacgtcag aaagttcgtc catcatctga ccggcatcct gcaaaatccg
     2701 ctgcgcccat tcataaatcc gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg
     2761 gtgtaacaac accacgttta gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc
     2821 cggtgaaacg cccagttctt ccgccacggc ggcaaaacca gcccgacgag ccaccagcat
     2881 aaagacgcgc aaatcattca gcagcggtaa attattcatg attcgtgttt tatgtttcac
     2941 cagttacggg gattaattcc ttttcagtca attataggat ggtgatgttg tcaattttga
     3001 tggtcaggaa gtgagaaccc aatgatgaaa acgatgcgta ttgctgcgat cccgggagac
     3061 gggattggca aagaagtcct tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc
     3121 tggggcttcg acttgagttt tgagcaaatg gagtgggcga gctgcgagta ttacagccat
     3181 cacggtaaaa tgatgccgga cgactggcat gagcaactta gccgtttcga cgccatctat
     3241 tttggtgccg tcggctggcc ggataccgtt ccggaccata tttcgttgtg gggttcgctg
     3301 ctgaaatttc gtcgtgaatt cgaccagtac gtcaacctgc gcccggttcg tctctttcct
     3361 ggcgttccct gcccgctggc gggaaaacag cctggcgaca tcgattttta cgtggtcagg
     3421 gaaaacaccg aaggcgaata ttcctcgctc ggcggtagag tgaatgaagg tacagagcat
     3481 gaagtcgtca ttcaggaatc ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat
     3541 gccttcgaac ttgcgcaaag ccgcccacgt aagacgctaa cttctgccac taaatcaaac
     3601 ggtttagcca tcagcatgcc gtactgggat gagcgagtgg aagcaatggc cgagaattac
     3661 ccggagatcc gctgggacaa gcagcatatt gatattctct gcgcgcgttt tgtgatgcag
     3721 ccggaacgct tcgatgtggt ggtggcgtcc aatttatttg gcgatatcct ttccgatctt
     3781 ggcccggcct gcaccggcac cattggcatt gccccatccg ccaacctgaa tccggaacgc
     3841 actttcccgt cgctcttcga gccagtccac ggttccgcgc cggatatcta cgggaaaaac
     3901 attgctaacc ctatcgccac gatttgggcc ggtgcaatga tgctcgattt tctcggcaat
     3961 ggcgatgagc gtttccagca agcgcataac ggtattctgg cagcaattga agaagtgatt
     4021 gctcacgggc cgaaaacacc tgatatgaaa ggcaatgcca ccacgccaca ggttgccgac
     4081 gcgatttgca aaattatttt gcgttaaggt caaaccagtt tatttgaacc gcgtcactga
     4141 cgcggttttt ttattcgttc tttgcagtaa ataacctgcg tcatttcacc ttttattgtt
     4201 tccgtttcgt gttttatggc tttccgtatt cttaattgtt taatttatgt aacatgcaaa
     4261 taatcaattt gttaaacaca acatacaact cattaccctg caaataatta caactggcaa
     4321 aagccagaga cgggctttta tgcccaggag atgactcatg atgagcaatg ttaagaaaaa
     4381 agatgtgccg ctgataagca tcagcctggt ggccattctt ttcatcgcag ctgcattaag
     4441 ccttttccca caacaatcgg ccgacgcggc caacgcaata tacacttttg ttacgcgtac
     4501 gttaggttcc gccgtacagg tattggtttt gctggcaatg ggactggtga tttatttagc
     4561 caccagtaaa tacggcaata ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc
     4621 ctggctgttt atgtttattt gtgccggttt aggttcttct acgctttatt ggggggttgc
     4681 tgaatgggcc tattattatc aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc
     4741 actcgaattt agcgtccctt actccttctt ccactggggc atcagcgcct gggcaactta
     4801 tacgctggcc tcattaatca tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag
     4861 tctttccggc attattgccg cgattaccgg cgttcgcccg caaggcccat ggggaaaact
     4921 ggttgatttg atgttcctga tcgccactgt cggcgcactg actatttccc ttgttgtcac
     4981 cgcagcaacc tttactcgtg gactttccgc gctgaccggt ttacccgata acttcaccgt
     5041 gcaggcattt gtgatcctgc tttccggcgg cattttttgc ctaagctcgt ggattggtat
     5101 caacaacggt ttgcaacgtc tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt
     5161 actggtgctg attgtcggcc caaccgaatt tattaccaac agcatcatca atgccatcgg
     5221 tctgaccacg caaaacttcc tgcaaatgag cttattcacc gatccgcttg gcgatggttc
     5281 atttacccgc aactggaccg ttttctactg gctgtggtgg atctcataca cccctggcgt
     5341 agcaatgttt ctcacccgcg tttcccgcgg tcgtaagatt aaagaagtta tctggggact
     5401 gatcctcggc agcaccgtcg gttgctggtt cttcttcggc gtaatggaaa gctatgccat
     5461 tcatcagttt atcaatggcg taatcaacgt cccacaggtg ctggaaacac tgggcggcga
     5521 aacagctgta cagcaagttc tgatgtcgtt gccagccggt aaattgttcc tcgccgcata
     5581 cctgggcgtg atgattattt tccttgcctc gcatatggat gcggtggcct acaccatggc
     5641 tgcgaccagt acgcgtaatc tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt
     5701 ctggtgcgtg gtgatcactc tgatcccgct ttccatcttg tttaccggtg cttcgctgga
     5761 aacgatgaaa accaccgtcg tgctcacagc ccttcccttc ctcgtcattt tactggtgaa
     5821 agtcggcgga tttattcgct ggctgaaaca ggattacgcc gacattccgg ctcatcaagt
     5881 tgaacattat ctcccgcaga caccggttga agccctggaa aaaacgccag tgctccctgc
     5941 gggaaccgta ttcaaaggcg acaactgagc gccatcatcc taacgataaa ggtatcccta
     6001 tgagcaatct gagccctgac tttgtactac ccgaaaatta ttgcgctaac ccgcaagagg
     6061 cgtggaccat tcctgcccgt ttttataccg atcagaacgc gtttgaacac gaaaaagaga
     6121 acgtcttcgc caaaagctgg atttgcgtcg ctcacagcag cgaactggcg aatgccaatg
     6181 attatgtgac gcgtgagatc attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg
     6241 ttttgcgcgc gttctataac gtgtgtccgc accgtggtca tcagttgttg agcggtgaag
     6301 gaaaagcaaa aaatgtgatt acctgcccgt atcacgcatg ggcattcaaa ctcgatggca
     6361 acctggccca tgcacgtaac tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac
     6421 tggttccggt gcgtctggaa gaatatgccg gattcgtctt catcaacatg gaccccaacg
     6481 ccaccagcgt agaagatcaa ttacccggcc tgggcgcgaa agtgctggaa gcctgcccgg
     6541 aagtccacga tctgaaactg gcggcccgct ttaccacccg cacgcctgcc aactggaaga
     6601 acattgtcga taactatctc gagtgctatc actgtggtcc ggcgcatcca ggtttctccg
     6661 actccgtaca ggttgatcgt tactggcaca ccatgcacgg taactggacg ctgcaatacg
     6721 gtttcgccaa accgtccgaa cagtcgttta aatttgaaga gggtacggat gcggcattcc
     6781 acggtttctg gctgtggccg tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga
     6841 cggtcattta tgaattcccg gtggattctg aaactaccct gcaaaactac gatatttact
     6901 tcaccaatga agagttaacc gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt
     6961 tccgtccgga agatttacgt ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct
     7021 atcgtggtca ggggcgcatc atggccgaca gtagcggtag tggcatttcc gaacatggta
     7081 tcgcccattt ccataatctg ctggcgcagg tgtttaagga ctaatgacat cggcggcggt
     7141 attttccgcc gctgggctga tttttgatgg agtacagcaa tgtcagacta tcaaatgttt
     7201 gaagtacagg tgagtcaggt ggaaccgctt accgaacagg tgaaacgctt cacgctggtg
     7261 gcaaccgatg gcaaaccgct gcctgcgttc accggaggaa gtcacatcat tgtgcagatg
     7321 agcgatggtg ataaccagta cagcaatgcg tattcactac tgagttcgcc gcatgacacc
     7381 tcttgttatc agattgccgt tcggctggag gaaaactcgc gcggcggttc ccgctttttg
     7441 catcagcagg taaaagtggg cgatcggtta acgatttcaa cgcctaataa cctgtttgcg
     7501 ctaattccct cagccagaaa gcatctgttt atcgcgggcg gtattggtat cacccctttc
     7561 ctgtcgcaca tggcagagct gcaacacagc gacgtcgact ggcagctaca ttactgctcg
     7621 cgaaatccag aaagttgcgc atttcgtgat gagctagtcc agcatccgca ggctgagaaa
     7681 gttcacttgc atcactcatc aaccggaaca cgactggaat tagcgcgatt attggcggat
     7741 atcgaacctg gcacacacgt ttatacctgt ggtcccgagg cgctaaatga agcggtaaga
     7801 agtgaagctg cgcgtctgga catcgtcgcc gatacgctgc actttgagca atttgctatc
     7861 gaagacaaaa ccggcgatgc ctttaccctg gtgcttgccc gttccggaaa agagtttgtg
     7921 gtgccggaag agatgactat tttgcaggtt attgaaaata ataaagccgc gaaagtggaa
     7981 tgtttatgtc gtgaaggggt atgcggaacc tgcgaaacag caatactgga aggtgaagct
     8041 gaccatcggg atcagtattt tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc
     8101 tgttgttcgc gtgcgaaggg taaacgcctg gtgttggatt tgtagtttgc caaagccgga
     8161 tgtggcgctg agcgcgccca gtccggcttc ggaagatttt actgcggata ttcctgcaat
     8221 aaattgtgta atgcttccgc catcagctca ccacgccagc cggaaatcag ctccggcaaa
     8281 ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat
     8341 gccagcaatt cggcgctgat cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc
     8401 gctttaaacg ctttacgata accaggcatg tccatcaggt taagcatcgg ctgcggtaag
     8461 gcctcttccg gcaatgtctg cgctttttcc accagcgcca gcagcgtttt accgtgaaag
     8521 cggatttcgc taccggataa acccaggctg tccagttcgc ctaaacttcc cggcatataa
     8581 cgcgcgaccg accacaaatg ctcttcacgc acgacaaagt tcaccgccag atcacgctct
     8641 cgcgccttgc gcagtcgcca gtcggctaac agttgcagac aggccagttg gcgtgtgcgt
     8701 aactgccagg cattggtgat atcacgccag gcatcttccg gcgcaacgac ttcctgacga
     8761 cgcatttgca tcaggcggca ttcatccagc gccgcaggta gccagccgga ggcctccgtt
     8821 tctaccataa gcttggcggt gatcggtaac agataccaga catccgccgc tgcgtattca
     8881 cactgacgtt cggtcagcgg tctggccagc cagtcggtgc gcgattcact cttgtccagc
     8941 gtaacgccgg aatactcttc caccatggaa gcgaaacccc atgacatcgg gcgtccgcag
     9001 aaggcagcaa ggatttgcgt gtcaatcaag ggttgtggta attcgccaaa gacattgagg
     9061 aacacttcca gatcttcact gcctgcatgg agaaattttg tgatggacgg atcgcgcagg
     9121 atcgctttca gcggtgacca gtcggtgatc ccgagaggat caattagcgc cagatgctcg
     9181 ccatcgaaaa gttgaatcaa ccccagctgc gggtaataag tgcgcgtacg aacaaattca
     9241 gtatccaggg ctatcgccgg aaaggcacgg acggcttcac acaaagaagc cagcgcatcg
     9301 tccgtggtaa tcatttggta attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg
     9361 ccggattaac cggcgtctga cgactgactt aacactcagg ctttattgtc cactttgccg
     9421 cgcgcttcgt cacgtaattc tcgtcgcaaa atttttccga cgttagattt cggtaactca
     9481 tcacgaaact ccaccagctt cggtactttg tagcccgtga gctgacggcg gcaaaaggtc
     9541 accagtgact cttcggtaag cgatggatct tttttcacta cgaagatttt caccgcttca
     9601 ccactggagc cggaaggtac gccaacagcc gcgacttcct gtacgccagg atgctgcatg
     9661 acgacatctt caatctcgtt gggatagacg ttaaaaccgg aaaccagaat catgtctttt
     9721 ttacgatcga caatgcgcag gaacccttct tcatccatca ccgcgatgtc gccggtgtgt
     9781 aaccagccat ttttgatgat ctcatctgta gcatccggac gctgccagta acccagcatc
     9841 acctgcggtc ctttgacaca aagctcaccc ggttgacccg gtggtacttc attatcatca
     9901 tcatccacca gtttggcttc cgtcgacggc accggcaaac cgatgctacc actatgataa
     9961 tcaatatcat atgggttaac gctgaccagc ggcgcacact cggtaaggcc atagccttcc
    10021 agcagatact gtcctgtcag tttcacccaa cgctctgcca ccacttgctg cactggcatc
    10081 cctccgcctg cggaaagatg cagactggag aaatccagct gctggaactc tttattgttc
    10141 agcaacgcat tgaacaaggt gttaacgccc gtgatagcgg taaacggata tttcgctaac
    10201 tcttttacca accctggaat atcgcgcggg ttagtgataa gcaggttctg cccacccagt
    10261 tcgataaaca gcaggcagtt aatggtcagg gcaaaaatgt gatacagcgg cagcgccgtc
    10321 accaccagct ctttgcccgg atgcaacagc ggaccatagg tcgcgttaac ctgttccagg
    10381 ttcgccagca tattgcggtg agtcagcatc gcgcctttcg ccacaccagt ggtgccgccg
    10441 gtgtattgca gaaaagctaa atcttccggc accagttcgg gtttgacgta ctgcatccgg
    10501 tagccgttat gcagtgcgct acgaaatgaa atggcatctg gcagatggta tttcggcacc
    10561 aaacgcttga tgtatttaac aacgaaattg actaccgtgc cttttgccgt agatagctga
    10621 tcgcccatac gggtcagaat tacgtgctga acggcggttt tatcaaccac tttttccagt
    10681 gtgtgagcaa agttagacac gataacaatc gccgatgcgc cgctatcgtt aagctgatgc
    10741 tcaagctcac gcggggtata caacgggtta acgtttacga cgatcatccc tgcacgcaaa
    10801 atgccaaaca gcgccaccgg atattgcaat aaattaggca tcatcaacgc aacgcgatcg
    10861 cctttcttca gccccaaccc ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc
    10921 agcttgcgga aggtcattac ctcccccata ttcacaaacg caggctgatc ggcgtagcgc
    10981 gcgaccgact gctcaaacat atctaccaga gattgataac ggtcagggtt gatctccgtc
    11041 ggaacgtccg cgggataacg gttaagccaa accttcttca attcttcacc tctaaaatgc
    11101 gtgttcgtcg tcatcgcaac cccaaatgat atacatgccg ttaacataat attaactcat
    11161 cataccagct tgataattac ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta
    11221 tctttaccgt aagaatgcag aaacagcgga ccagccgctg tttctttttc tttaaaaaca
    11281 agcaattatt cagttacaac tgtttgtact ctcgcgggac cgggattata ccagccccat
    11341 ccgccgtagc catagggcca gccacgaccg ccataaaacc atggatcaat cggctgaggc
    11401 ggcataatca cctgctgggt taaatgccaa cgtttgtaac ccgttacttg catcaccata
    11461 aatttatagg gcgtattgcc gattttgccg tcaaccgcac cagtgattgg cccaactacc
    11521 gtaaccagtt gtccacggaa atccaccggg tccagaaaac cgttcacatc ggcataaatg
    11581 cgaccgcgag aaggttctcc cagcgtcggt ctggctccgc tgtccagcgg tacggtagca
    11641 atttccaggc gggttttccc ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt
    11701 gcctcctgac caacgtacag ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc
    11761 gtgggactgc tgcctttaat ggcgtccggc acagtgacac aaccgctcag cattagcgca
    11821 aacgtacctg ccagtatgcc tttgataaca ttcttttgaa ccgccatgat gcgactcctt
    11881 tttttctcag ggcatactct taagattcat tctttgcccg gaagtttctt ccatgcgacg
    11941 ttgttacgta aataaaccgg ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac
    12001 atctgacacg caatcggcag catatcttcc gccgctggca gtaaaacttc tccgtcgcgc
    12061 aaaaccagcc cgctctcttt accgagatcc ggccaggctt gccagcccgt ccctaccgtt
    12121 acccattcgc cggaaagctg ttgcattcgt tcatgaacga gttcgggttt gagtacggct
    12181 tcggtttctt caccgtgcca gataccgttt tcatcacgct gatattcggc ccagtaaact
    12241 tcgcccattc gcgcgtcaat ggctgccagc acgcgggttg cgccgttttt gcgccacgcc
    12301 ccttgcgcca tcgtcattag cgtggagacg ccaatcatcg gtagctctgc gccaagtgcc
    12361 agcccttgcg caatgccaat accaatgcgc acgccggtaa agctaccggg gccgcgcccg
    12421 taagccagag cgttaatatc agtcagggaa gttccgctgg tggtcaggat atcctgcacc
    12481 atcggtaaga ttcgttgagt atgttcacga gggcaaagct caaaatgagc gttgacagta
    12541 ccgtcgttcc acagggcgac agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc
    12601 atgggtcttc gtgcttagat caataaaaag gcgcgcatca taccatactc cgtaacaaat
    12661 tacctggagg atggtatcgc aaggaaacga accgcacggg caatgtcacg agtgcgtggc
    12721 gcgggcggca gactggcgag aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca
    12781 caaatcacca gcacgccacg atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg
    12841 agagtaatga cggcatctgg tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga
    12901 caatcttcca tgcgcgcttt taacagtgga tcatccggcg aggtaaacgg caacttgtcg
    12961 ataatcacca atgacaatgt atcgccacgc acgtccaccc cttcccagaa actactggtt
    13021 gccacaagaa gcgcgttacc ggcgctgaca aattgctgca acagttgccc tttgctggtt
    13081 tccccctgca acaaaacggg aagcgtcatg gtagcgcgga actgctcggc cagatcacgc
    13141 atcatggcgt gcgaggtaca aagcataaaa caacgaccgt tgttagcttc gatgatcggt
    13201 cgcagcattg ccgctaactg gcgagcagaa cctggctggt tggtttgcgg cagattgcgc
    13261 ggcacacaga gtaacgcctg gcggctgtaa tcaaacgggc ttggcaatag caacgactcc
    13321 gcctgttcta tcccaagccg cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt
    13381 gctgaggtga agatccagct accaggcttt tgcgccatta actctttgaa tttatccgcc
    13441 acgctgagcg gcgtgagagc cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg
    13501 tagcccggct gattgatctc ttttagccgc ttcagccgtg tgcgatacaa cgtagcgcgc
    13561 tcaaatgccg catccagcaa ggcggaacgc cccagtgaca gtttcgccac gtcataacaa
    13621 agttccaggg tgtcatcgag cagtaaaaat gcccgctgaa tttgcggatt agctaacagc
    13681 tcacgcaggt taccgcgata acctggctca ccgagttgca gacgaaaatc ctgcgcactc
    13741 tgggcaagac gatcagcgca cttttgtaac tgctgggtgt cttttaattc ggtgcggtaa
    13801 gcgatggtga tgtcttttgc caggtcgagc agttgtcgac tggagagtga ctgaccaaaa
    13861 tactggctgg caatgtccgg tagctggtgg gcttcgtcga agatcatgac gtccgcttcc
    13921 gggatcaact cgccaaatcc actctcttta accaccatat ccgccagaaa gagatgatgg
    13981 tttaccacca ccacatcggc gtccatcgct tttttacgtg ctttgaccac aaagcaatct
    14041 ttatacatcg ggcagtcgct gccaagacag ttgtcgttgg tgctggtaac cagcggccac
    14101 gcctgtgaat cttccgccac gctgacgcag gtgctgatat caccatcgac tgtttgatta
    14161 gaccaggagc gcagcaggat cacatcgctt aagatttgta ccggcagatc gccccccgcc
    14221 agcgcctgct gttcgagacg ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc
    14281 acgttgcccg tatatttcaa tgcctttgag actgttggca aatcgcggct gtggagctga
    14341 tcctgcaacg cttttgagcc ggtcgagata atgactttct ttttcgcccg cagcgcagga
    14401 gccaggtaag cgtaggtttt gcccgtaccg gttcctgctt ccaccaccag cggctggcct
    14461 ttttctatcg cctgggtgac ggctaccgcc atctgtcgct gtggttctcg cggcttaaag
    14521 cctggtatcg ctttcgccag ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct
    14581 gttgatttga acagggatta tgtcaggatg agggcgcttt cgccagttga agtggtgacg
    14641 gcaacctcac attgtggcag tctttgcagc acgaaaatgg aagtataatg aggacaaaat
    14701 gatgactatc gttcgtatcg atgctgaagc ccgctggtct gatgtagtaa tccacaacaa
    14761 cacgctctac tacactggtg taccggaaaa cctcgacgcc gatgcttttg agcaaaccgc
    14821 caacacgctg gcgcagattg acgccgtgct ggaaaaacag ggcagcaata aatcgagcat
    14881 tctggatgcc accattttcc tggctgataa aaacgacttc gcggcgatga ataaagcgtg
    14941 ggatgcctgg gttgtggcgg gtcatgcgcc ggtgcgctgc acggtgcaaa cggggctgat
    15001 gaacccgaag tataaagttg aaattaagat tgtggctgcg gtgtaagctt tatcgaagca
    15061 aaataagtca gacgataatt tatcgataat actggtcggt tttacataaa tcgaccagag
    15121 attaagatat tactgttgat gctcctgcta attcattatt tatttttata atttcaattt
    15181 tagctataaa gttaactata taaataaaca taaccatgcc gttgtgttcg ccctctgact
    15241 agcaactctg gttttctttc ttagctgaat aatctggtta tagcgtgagg actgaccgtt
    15301 aaatttctcc atcttactgg ataacacacc atgagctgct acctttaaaa cacctgaatg
    15361 tcaacaggtt aactcgtgcc atatcgttat aatccctttg atattagatg caaattaagg
    15421 tcatatatag tcttatcgat aacatggtta attttaagga taagaatatg cctgctgtaa
    15481 tagataaagc cctggatttc attggtgcca tggatgtatc agcgccaaca ccaagttcga
    15541 tgaatgaaag cacggcgaag ggaatcttta aatatttaaa agaactggga gtacccgcga
    15601 gtgccgctga tattacggcg cgagctgacc aggaaggctg gaaccctggg ttcacggaaa
    15661 aaatggttgg atgggcaaaa aaaatggaga caggtgaacg ctctgtgatt aaaaatcctg
    15721 aatacttttc aacatatatg caggaagaac taaaagcact ggtctgagtt aaatttatat
    15781 cagcataaat gggtcaggac gcttttaatc acatataaaa agcgtccttt ttctcacaat
    15841 caaatgatca tcggcttcta taacgaaatc gatacggatt agtgtgttac tcgtcttcat
    15901 cctcaaaacg cgccacgatt cgctcgccgg agtggttggc acgcagctct tctgccacca
    15961 gcgcgattgc ctggccgctg ctcatccctt gtgccatcag ttcctggatc cgctcgacag
    16021 ctttttgctg ctgttcatgg gtgagtgaag gtaaacctgc aaacattgtt aactcctgct
    16081 aaattgttgg cgctaattat ttcatgctac ccggcacata gccagtagag tcaggactga
    16141 tgaagacgtt atctcccgct gtgattactt taccctggcg tcaggacgcc gctgaatttt
    16201 atttctcccg cttaagccac ctgccgtggg cgatgctttt acactccggc tatgccgatc
    16261 atccgtatag ccgctttgat attgtggtcg ccgatccgat ttgtacttta accactttcg
    16321 gtaaagaaac cgttgttagt gaaagcgaaa aacgcacaac gaccactgat gacccgctac
    16381 aggtgctcca gcaggtgctg gatcgcgcag acattcgccc aacgcataac gaagatttgc
    16441 catttcaggg cggcgcactg gggttgtttg gctacgatct gggccgccgt tttgagtcac
    16501 tgccagaaat tgcggaacaa gatatcgttc tgccggatat ggcagtgggt atctacgatt
    16561 gggcgctcat tgtcgaccac cagcgtcata cagtttcttt gctgagtcat aatgatgtca
    16621 atgcccgtcg ggcctggctg gaaagccagc aattctcgcc gcaggaagat ttcacgctca
    16681 cttccgactg gcaatccaat atgacccgcg agcagtacgg cgaaaaattt cgccaggtac
    16741 aggaatatct gcacagcggt gattgctatc aggtgaatct cgcccagcgt tttcatgcga
    16801 cctattctgg cgatgaatgg caggcattcc ttcagcttaa tcaggccaac cgcgcgccat
    16861 ttagcgcttt tttacgtctt gaacagggtg caattttaag cctttcgcca gagcggttta
    16921 ttctttgtga taatagtgaa atccagaccc gcccgattaa aggcacgcta ccacgcctgc
    16981 ccgatcctca ggaagatagc aaacaagcag aaaaactggc gaactcagcg aaagatcgtg
    17041 ccgaaaatct gatgattgtc gatttaatgc gtaatgatat cggtcgtgtt gccgtagccg
    17101 gttcggtaaa agtaccagag ctcttcgtgg tggaaccctt ccctgccgtg catcatctgg
    17161 tcagcactat aacggcgcga ctaccagaac agttacacgc cagcgatctg ctgcgcgcag
    17221 cttttcctgg tggctcaata accggggctc cgaaagtacg ggctatggaa attatcgacg
    17281 aactggaacc gcagcgacgt aatgcctggt gcggcagcat tggctatttg agcttttgcg
    17341 gcaacatgga taccagcatt actatccgca cgctgactgc cattaacgga caaatatact
    17401 gctctgcggg cggtggaatt gtcgccgata gccaggaaga agcggaatat caggaaactt
    17461 ttgataaagt taataagata ttacgccaac tggagaagta agacgtggaa taccgtagcc
    17521 tgacgcttga tgatttttta tctcgctttc aacttttgcg cccgcaaatt aaccgggaaa
    17581 ccctaaatca tcgtcaggct gccgtgttaa tccccatcgt ccgtcgaccg caaccggggt
    17641 tgttgctgac tcagcgttca attcatctgc gtaaacacgc tggacaagtg gcattccctg
    17701 gaggtgcagt cgatgacacg gacgcatcag ttatcgccgc cgcgctgcgc gaagctgaag
    17761 aagaggtcgc tataccgcct tccgccgttg aagttatcgg cgtgctgccg cccgtcgata
    17821 gcgtaactgg ctatcaggta accccagtgg tcggcattat cccgcccgat ctgccgtatc
    17881 gcgccagtga agatgaagtc tcggcggtgt ttgaaatgcc gctcgcccag gcattacatc
    17941 tgggtcgtta tcacccttta gatatctacc gccgtggcga ttcacatcgg gtatggctgt
    18001 cctggtacga acagtatttt gtatggggaa tgaccgcagg cataattcgt gagctggcgc
    18061 tgcaaattgg tgtgaaaccc tgactatact tatctttaca tctacaaaac actacttgag
    18121 acaatcatcg caatattagt taaatcgcgg tttttgatta gtttaattca tgtgaatagt
    18181 taagccagtc gccgcgttcc ctcttacact atgcgctgtt attagttcgt tactggaagt
    18241 ccagtcacct tgtcaggagt attatcgtga ttagtctatt cgacatgttt aaggtgggga
    18301 ttggtccctc atcttcccat accgtagggc ctatgaaggc gggtaaacag ttcgtcgatg
    18361 atctggtcga aaaaggctta ctggatagcg ttactcgcgt tgccgtggac gtttatggtt
    18421 cactgtcgct gacgggtaaa ggccaccaca ccgatatcgc cattattatg ggtcttgcag
    18481 gtaacgaacc tgccaccgtg gatatcgaca gtattcccgg ttttattcgc gacgtagaag
    18541 agcgcgaacg tctgctgctg gcacagggac ggcatgaagt ggatttcccg cgcgacaacg
    18601 ggatgcgttt tcataacggc aacctgccgc tgcatgaaaa cggtatgcaa atccacgcct
    18661 ataacggcga tgaagtcgtc tacagcaaaa cttattattc catcggcggc ggttttatcg
    18721 tcgatgaaga acactttggt caggatgctg ccaacgaagt gagcgtgccg tatccgctca
    18781 aatctgccac cgaactgctc gcgtactgta atgaaaccgg ctattcgctg tctggtctcg
    18841 ctatgcagaa cgaactggcg ctgcacagca agaaagagat cgacgagtat ttcgcgcatg
    18901 tctggcaaac catgcaggca tgtatcgatc gcgggatgaa caccgaaggt gtactgccag
    18961 gcccgctgcg cgtgccacgt cgtgcgtctg ccctgcgccg gatgttagtt tccagcgata
    19021 aactgtctaa cgatccgatg aatgtcattg actgggtaaa catgtttgcg ctggcagtta
    19081 acgaagaaaa cgccgccggt ggtcgtgtgg taactgcgcc aaccaacggt gcctgcggta
    19141 tcgttccggc agtgctggct tactatgacc actttattga atcggtcagc ccggacatct
    19201 atacccgtta ctttatggca gcgggcgcga ttggtgcatt gtataaaatg aacgcctcta
    19261 tttccggtgc ggaagttggt tgccagggcg aagtgggtgt tgcctgttca atggctgctg
    19321 cgggtcttgc agaactgctg ggcggtagcc cggaacaggt ttgcgtggcg gcggaaattg
    19381 gcatggaaca caaccttggt ttaacctgcg acccggttgc agggcaggtt caggtgccgt
    19441 gcattgagcg taatgccatt gcctctgtga aggcgattaa cgccgcacgg atggctctgc
    19501 gccgcaccag tgcaccgcgc gtctcgctgg ataaggtcat cgaaacgatg tacgaaaccg
    19561 gtaaggacat gaacgccaaa taccgcgaaa cctcacgcgg tggtctggca atcaaagtcc
    19621 agtgtgacta atacttctta ctcgcccatc tgcaacggat gggcgaattt atacccgctt
    19681 tctcgtctgc tgtaatattc cccactacac ttccactgtt gcgtcaggcg tttgtcgcca
    19741 tacgcttaca gggtggcccg catgcaaaaa gcacaacgga tcattaaaac ctatcgccgt
    19801 aatcgaatga ttgtttgtac gatttgcgca ctcgttacgc tcgcttcgac cctgagcgtg
    19861 cgatttattt cacagcgtaa cttaaatcaa caacgggtag tacaattcgc caatcacgct
    19921 gtagaggaat tagataaagt actgcttccc ctacaggcag gtagcgaagt cttgcttccg
    19981 ctgattggtc tgccctgctc tgtcgcccat ttgccattac gtaaacaggc ggcaaaactc
    20041 caaactgtgc gatccattgg cctggtgcaa gacggcacac tttattgctc cagcattttt
    20101 ggttatcgca atgtgcccgt cgtggacatt ctggctgaac ttcctgcacc gcaaccactt
    20161 ttacgcctga cgatcgaccg tgccctgatt aaaggcagtc cggttttgat tcaatggacg
    20221 cctgcagcgg gcagtagcaa tgctggggtc atggagatga ttaacatcga cttactgacg
    20281 gcaatgctcc ttgagccaca actgccgcaa atcagtagcg ccagcctgac ggtggacaaa
    20341 cggcatttgc tctatggtaa tgggctggta gattcccttc cgcaacctga agacaatgaa
    20401 aactatcagg tttcttcgca acgctttcct tttaccatta acgttaatgg tccgggggct
    20461 acggcgctgg catggcacta tcttccaaca caattaccgc tggcggtgct gctaagttta
    20521 ctggtgggct acattgcctg gctggcgacc gcttaccgga tgagcttttc ccgcgaaatc
    20581 aatctgggcc tggcgcaaca tgagttcgaa ttgttctgtc agcctttgct taatgcgcgc
    20641 agccagcaat gtattggtgt agagattttg ctgcgctgga acaatccgcg tcagggctgg
    20701 atttcaccgg atgtgtttat tcctatcgcg gaagaacatc atttaattgt gccactgacc
    20761 cgctatgtga tggcagaaac cattcgtcag cgccatgttt tcccgatgag tagtcagttt
    20821 catgttggca ttaacgtcgc acccagccat tttcgccgtg gtgtgctgat aaaagatctc
    20881 aatcagtact ggtttagcgc tcacccgatt cagcaactga tcctcgaaat caccgaacgc
    20941 gatgccttac tggatgttga ttatcggatt gcccgcgagc tacatcgtaa aaacgtcaaa
    21001 ctggcgattg atgacttcgg caccggcaac agttcatttt cctggcttga aacattacgt
    21061 cctgacgtgc tgaaaattga taagtcattt accgcagcta taggttctga cgcggttaac
    21121 tcgacggtga ccgatatcat catcgcgctg gggcaaagac tgaatattga actggtggcg
    21181 gagggcgtgg aaacgcaaga acaggcgaag tatttgcgcc gtcatggcgt gcatattttg
    21241 caagggtatt tgtacgcaca gccgatgccg ctacgtgatt ttcccaaatg gctggcgggc
    21301 agccaaccgc cgcccgcccg gcataatgga catatcacgc ccattatgcc gttacgttaa
    21361 cgcttactca tcttcatcgt gcgccggttg ctctttaaca atgcgaacca gatcaacacg
    21421 ataatcattg gcttcaatga tggtgatatg cagtggccct acatcaatca catcgcccac
    21481 acggggaatg tgaccatttg ccgagatcac caggcccgcg accgtcgcga tatcatcgtc
    21541 atcggcaagg tgctcaacat caagcgcctg ctgcaaggca tgcaaatctg taccgccttt
    21601 taccagccag ccgtcaccat cggtaatgat ttccggcgtt tcgtcagcgt ccgggaattc
    21661 acccgcaatg gcttccagca catccagcgg cgtgaccaag ccttgtacca caccaaactc
    21721 gttggtcacg ataacaaagc tcccgcgagc gcgacgcagc acgcccagca ggttgatcgg
    21781 atcgagggtt tccgggacga taatcgccgg agacgccgaa gcaatcgccg ccacatcaac
    21841 gccctcttcc agcgccacca gcagttcttt agcacgtacg attccgatga tttcatccag
    21901 ttcaccgcga cataccggga acaggctgtg cggtgaggag agcagctgct cgcggatttc
    21961 atcgaccccg agattagcgt caacccagct tatttcaccg cgcggcgtca tgatcccgcg
    22021 cagagaacgc gacgccagcg tcagtacgcc gttaatcatg taacgttctt cttcggcaaa
    22081 tgcaccttcc gggatcggca tcggcatcgg gttatcggca tcgtgctgaa cattggcctg
    22141 acgtttcccg cccatcaaac gcaggatggc atcggcagta cgcgctcgca gcggcaaagt
    22201 cgactggtga cgaataaagt tgcgacgcgc aatctggtta aacacttcga tgatgatcga
    22261 gaagccaatc gcggcataca ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac
    22321 cagactcaga ccaatcatta acaggaagct cagacagagc accaccaccg tggggtgctg
    22381 gttaacgaat cgcgtcagcg gtttggatgc cagcaacata accgccatcg caatcactac
    22441 cgccgccatc atcaccggca gatggttaac catccctact gcagtaatta ccgcatccaa
    22501 cgagaagacg gcgtcaagga tgacaatctg tgtgacgacc acccagaaac tggcgtagcc
    22561 tttaccgtgg ccggaatcat gatcgcggtt ttccagccgt tcatgcagtt cggttgttgc
    22621 tttgaacagc aagaatatcc ccccgaacaa cataatcagg tcgcgtccgg agaaggagaa
    22681 atccatgacg gtaaatagcg gtttggtcag cgtgaccatc catgaaatca gcgacagcag
    22741 ccccagacgc ataatcagcg ccagtgataa ccccagcaaa cgcgctttat cgcgttgttt
    22801 tggcggcagt ttgtcagcaa gaatggcgat gaagaccagg ttatcgatac ccagcacaat
    22861 ttcgagaaca acaagcgtga gtagccccgc ccaaattgag gggtccatta agaattccat
    22921 gacaagctcc tgcttaagga atagctattc gacgccagaa ataatgcagg cgtaacgaca
    22981 aaatgcaaac gaaaggtgcg gcatagagag ccagaaaggc aggcgttaaa aggcctgatg
    23041 ctgaaatgac gtcggtgacg atccatactg cgggctactg ccctatactc catggttgtt
    23101 aaacgggagt taaacatatc agagacgcct ctgatttggc aaagatttac cttcctttgc
    23161 aaacgaatgt gacaaggata ttttaccttt cgaaatttct gctaatcgaa agttaaatta
    23221 cggatcttca tcacataaaa taattttttc gatatctaaa ataaatcgcg aaacgcaggg
    23281 gtttttggtt gtagccctta tctgaatcga ttcgattgtg gacgacgatt caaaaataca
    23341 tctggcacgt tgaggtgtta acgataataa aggaggtagc aagtgaccat tgctattgtt
    23401 ataggcacac atggttgggc tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc
    23461 gagcaggaaa acgtcggctg gatcgatttc gttccaggtg aaaatgccga aacgctgatt
    23521 gaaaagtaca acgctcagtt ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt
    23581 gatacatggg gaggcagccc gttcaatgct gccagccgca ttgtcgtcga caaagagcat
    23641 tatgaagtca ttgcaggcgt taacattcca atgctcgtgg aaacgttaat ggcccgtgat
    23701 gatgacccaa gctttgatga actggtggca ctggcagtag aaacaggccg tgaaggcgtg
    23761 aaagctctga aagccaaacc ggttgaaaaa gccgcgccag cacccgctgc cgcagcacca
    23821 aaagcggctc caactccggc aaaaccaatg gggccaaacg actacatggt tattggcctt
    23881 gcgcgtatcg acgaccgtct gattcacggt caggtcgcca cccgctggac caaagaaacc
    23941 aatgtctccc gtattattgt tgttagtgat gaagtggctg cggataccgt tcgtaagaca
    24001 ctgctcaccc aggttgcacc tccgggcgta acagcacacg tagttgatgt tgccaaaatg
    24061 attcgcgtct acaacaaccc gaaatatgct ggcgaacgcg taatgctgtt atttaccaac
    24121 ccaacagatg tagagcgtct cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt
    24181 ggtatggcat tccgtcaggg taaaacccag gtgaataacg cggtttcggt tgatgaaaaa
    24241 gatatcgagg cgttcaagaa actgaatgcg cgcggtattg agctggaagt ccgtaaggtt
    24301 tccaccgatc cgaaactgaa aatgatggat ctgatcagca aaatcgataa gtaacgtatt
    24361 gtgttgatta tcactcagtt ttcacactta agtcttacgt aaacaggaga agtacaatgg
    24421 agattaccac tcttcaaatt gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat
    24481 caatcctcga tgaatttcag tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg
    24541 ttcttgggga tatgaaaacc ggtattatta tcggtggtac gctggaaatg atcgcgctgg
    24601 gctggatgaa catcggtgct gcagttgcgc ctgacgccgc tctggcttct atcatttcta
    24661 ccattctggt tatcgcaggt catcagagca ttggtgcagg tatcgcactg gcaatccctc
    24721 tggccgctgc gggccaggta ctgaccatca tcgttcgtac tattaccgtt gctttccagc
    24781 acgctgcgga taaggctgct gataacggca acctgacagc gatttcctgg atccacgttt
    24841 cttctctgtt cctgcaagca atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg
    24901 ttggtaccag cgaagtacag aacatgctga atgcgattcc ggaagtggtg accaatggtc
    24961 tgaatatcgc cggtggcatg atcgtggtgg ttggttatgc gatggttatc aacatgatgc
    25021 gtgctggcta cctgatgccg ttcttctacc tcggcttcgt aaccgcagca ttcaccaact
    25081 ttaacctggt tgctctgggt gtgattggta ctgttatggc agtgctctac atccaactta
    25141 gcccgaaata caaccgcgta gccggtgcgc ctgctcaggc agctggtaac aacgatctcg
    25201 ataacgaact ggactaacag gtgagcgaaa tggttgatac aactcaaact accaccgaga
    25261 aaaaactcac tcaaagtgat attcgtggcg tcttcctgcg ttctaacctc ttccagggtt
    25321 catggaactt cgaacgtatg caggcactgg gtttctgctt ctctatggta ccggcaattc
    25381 gtcgcctcta ccctgagaac aacgaagctc gtaaacaagc tattcgccgt cacctggagt
    25441 tctttaacac ccagccgttc gtggctgcgc cgattctcgg cgtaaccctg gcgctggaag
    25501 aacagcgtgc taatggcgca gagatcgacg acggtgctat caacggtatc aaagtcggtt
    25561 tgatggggcc gctggctggt gtaggcgacc cgatcttctg gggaaccgta cgtcctgtat
    25621 ttgcagcgct gggtgctggt atcgcgatga gcggcagcct gttaggtccg ctgctgttct
    25681 tcatcctgtt taacctggtg cgtctggcaa cccgttacta cggcgtagcg tatggttact
    25741 ccaaaggtat cgatatcgtt aaagatatgg gtggtggctt cctgcaaaaa ctgacggaag
    25801 gggcgtctat cctcggcctg tttgtcatgg gggcattggt taacaagtgg acacatgtca
    25861 acatcccgct ggttgtctct cgcattactg accagacggg caaagaacac gttactactg
    25921 tccagactat tctggaccag ttaatgccag gcctggtacc actgctgctg acctttgctt
    25981 gtatgtggct actgcgcaaa aaagttaacc cgctgtggat catcgttggc ttcttcgtca
    26041 tcggtatcgc tggttacgct tgcggcctgc tgggactgta agactgttgt acactaccgg
    26101 ggccttttgg ccccgttttt ttatctggag gattaatgac aatcacggac ctggtactga
    26161 ttcttttcat cgccgcactc ctggccttcg cgatctacga tcagttcatc atgccccgcc
    26221 gtaacggccc caccctgctg gcaattcctt tgctccggcg tggtcgcatc gatagcgtta
    26281 tcttcgtcgg attgattgtc attcttatct ataacaacgt cacgaatcat ggtgcgttaa
    26341 taacgacatg gttattaagc gcactggctc tgatgggttt ttatatattc tggatccgcg
    26401 ttccgaagat catctttaaa caaaaaggtt ttttcttcgc caatgtctgg attgaatata
    26461 gccgaatcaa agcgatgaac ttgtcggaag atggcgtgtt ggtgatgcaa ttagaacagc
    26521 gtcggctgtt aatccgcgtt cgaaatatcg acgatctgga aaaaatttat aagcttctcg
    26581 tttcaactca ataagttatg aatttagcca aagctatgtt tagtgtattt ttaataatca
    26641 gacatagctt aggctatatt acctcttccc ttatttgtta tttattttaa cgtttcattg
    26701 atatataaat ctaaatgtaa atcgttatca ataaagcaat gaaataatat attccaacag
    26761 ttgttttata ttctcaaaat atgttaaggt tgcgccctca tttggggagt agccgatttc
    26821 cagattccgg aaatgtacgt gtcaacatac tcgttgcaaa acgtggcacg tacggactga
    26881 atactttcag tcaggcgaga ccatatgcac atcaatcgct atgcctgcat gaacgcgaaa
    26941 accatcgcgg cttgttgttc atgtatgcat gtttgctggg ggcgatgatg tgctttatgg
    27001 ataccccggt caggacattg tcatgaatat cactgctact gttcttcttg cgtttggtat
    27061 gtcgatggat gcatttgctg catcaatcgg taaaggtgcc accctccata aaccgaaatt
    27121 ttctgaagca ttgcgaaccg gccttatttt tggtgccgtc gaaaccctga cgccgctgat
    27181 cggctgggga atgggcatgt tagccagccg gtttgtcctt gaatggaacc actggattgc
    27241 gtttgtgctg ctgatattcc tcggcgggcg aatgattatt gagggttttc gtggcgcaga
    27301 tgatgaagat gaagagccgc gccgtcggca cggtttctgg ctactggtaa ccaccgcgat
    27361 tgccaccagc ctggatgcca tggctgtggg tgttggtctt gctttcctgc aggtcaacat
    27421 tatcgcgacc gcattggcca ttggttgtgc aaccttgatt atgtcgacat tagggatgat
    27481 ggttggtcgc tttatcggct caattattgg gaaaaaagcg gaaattctcg gcgggctggt
    27541 gctgatcggc atcggcgtcc agatcctctg gacgcacttc cacggttaat aagaacgctg
    27601 ccagaggtga atattaaagt ccgtctggca gtcgaacact tcttttgctg ccagtgtttg
    27661 ccagacttct ggcttcgcac gccaggcaaa cggcgtcatc tgcaataatg cgacggcttc
    27721 atcaccgcga agacgcatcg gataacacaa ctcatcactc tgctgtaatg taaaaccttc
    27781 cagttgttct gcatgaggtg catgaagatg tacttcattg taaatcagcc ccttcagctc
    27841 catcaaatgt cgcggtcccg gcgtggcagt aatgacccag ccgccgggct tcactactcg
    27901 tgctaattct tctgctttac acggcgcgta aatacgtatt atggcgtcca tactggtatc
    27961 ggaaaacggc aaacggtggc tggaagcgac acaaagagtg acctgcggat agcgtttcgc
    28021 cgccgctttt atcgctacct tcgagacatc caggccaaac gtggtgattt cgggcaacac
    28081 atcggcaaat gcgtgtgtgt aatacccttc accacagcca atatccagca ccgccgtggc
    28141 cttttcatca agccgttccc gcagttgggc gacaattgca tcacgcagcg gctgataatg
    28201 tccggcatct aagaatgcgc ggcgtgcttg tatcatttcc gcgctgtcgc ccggatcacg
    28261 agaccgttta tgctgaacgg gcagcagatt gacataccct tctttcgcca tatcaaactg
    28321 atgtcgctgg ggacagatat agctgttttt ttcacgcgaa agaggctgat ggcaaagtgg
    28381 acaggaaaac gacatgacaa ctccggcagg atactaaagg ccgcaagtgt aacgcgaatc
    28441 acgcggcggg caaatatccg aacgccagga gcgaagataa agcatagtaa aaagcctcgc
    28501 attcgacgag gctttatagc gagattgaag cgtattcaca cttcagatca gtggattcga
    28561 tcagatagct gttacgttaa cagctgccgg acctttctgg ccgtcctgaa tttcgaactc
    28621 aacgttctga ccttcagcca gagttttgaa gccattaccc tggatagcgg agaagtgtac
    28681 gaacacatct ttgctgccat cagccggagt aatgaagcca aaacctttag actcgttgaa
    28741 ccacttaacc tgacctttaa tctttgccat ttgaaaaatt ccttagattg ttttcttcgc
    28801 ccgcaggcat aacatagata aaactgacac attactgcat gaggcaccaa tataaggctc
    28861 ggcagagaag cggtattcaa cgtcaacgtg tttactcagg acttctttac tgaaaatgcc
    28921 acacataaac agaactgtac ctcgtttaac ccgaaatctg ttatcacata caacgttaat
    28981 tatggcaagc catttttaaa catgtctcga tcagacgcac aaatctcgat acgtcttcca
    29041 ctttttttgc acacttatgc aacggaatac gcgccgatat atcattgtgc ttaaccttgc
    29101 cagttcaggc agatacttaa actggcgtat tttctaacat agttcaataa gttcagttgt
    29161 tccaggccag tgggagaagt tattacatag tgcgtgcaat atcacatttt ttgctatgca
    29221 atgaataaaa agttatatca ctttttctca taaaacagtc agttaacggc tatcaattac
    29281 cctaaagaga agtcaatccc caaagggatt gaaaatttaa aataagaaaa actgatgaat
    29341 gagcaaaaaa atcaagagag aaacgtttcg ctaataaata ataccacgct taatcacaaa
    29401 tccgatgtaa acatcctaat aaattaatga ggaaaatatc tttttctgat atcccgttac
    29461 gcaaataaat aatatgataa ttatttgaag tcagaccaga gttttaattt gaagcagttg
    29521 caccatggca gtgcgtataa tcaggatgaa ttgagtaacc cttcaacgac ggggttactc
    29581 aagattgagg aaggattatc gttcggcgac cagacgaata atatcttcgt cttcaaccga
    29641 tttttgactc tcaacggtct ttttatctgc tttctcaggt tcgtttgctt cgcacaaacg
    29701 acgtaacagt gcattttgac gtttttgctg atccagcaac gcctcaagca gttcaatctg
    29761 ttcgttagtc cgagaactgg cacgattaat aaaaaaccac aagatgagcc cgataagaag
    29821 aaccaccacc gatacaacca aagacgcaat gttcatcacg cctgaattta caacttcgtt
    29881 catttcacca cctctgagta gaggcgctat tctaccactg ctggagagga agaaaatcta
    29941 gtgctgaaaa aatgatatca ccacgggata aactggttaa tggcacaaat tccgctgaaa
    30001 aattgtacat cctgatcgca catcatgttg aatacctgcg cccaaagcag caagcaagcc
    30061 aacaccacga caaccagaac gacccatcga aactttttca ctccaatctc cgtttcacct
    30121 accttatgtc attaaactag catgcgttag ttaaacagcg cctaactatg tcgatattcg
    30181 tgctttttcg gaatgagtcg cttgcttcag cgcatattgc cgctacgatt aagcgaacat
    30241 aaaaagagaa gaggttgtaa tgcgattcat cattcgcaca gttatgctga tagcactggt
    30301 atggatagga ttattactca gcggatatgg cgttttgatt ggaagcaaag agaatgccgc
    30361 aggattaggt ttgcaatgta cctatctgac tgccagaggc accagcacgg tacaatactt
    30421 gcacactaaa agtggtttct tggggataac agattgccca ctattacgta aaagcaatat
    30481 cgtggtcgat aatggttaag ggtaataaat aaccagattc tcactcatga gtaaaatatt
    30541 cttatagcaa gcgcgtttaa tataacgcac cttcaacttt atttttctca tgctaaataa
    30601 agaacctgtc tgtttgatat tccgacaggt tcttactctc atcagaacgg atagtcgtga
    30661 taacccattt gggcagaaat tttgcgcgct gcggtatgca acattgcgac atattcctgt
    30721 aaacgctctt cagagaaacg caacgtcggg aaggagatgc tcaaaccggc aatgaccacg
    30781 ccaaagcgat caaataccgg taccgcaatg catcgcagcc cttcttcctg ctcttcgtta
    30841 tcttcgccat acccctgctc gcgcacctgg tccagaacgg gtaataacgc ttctgtactg
    30901 gtgatggtcc gctcggtact gcgtttatac tccacgccct caagaatttg cttcacttca
    30961 tcgagatcgc gccatgccag cagtacctta ccaatcgcgg tgctgtacag cggattacga
    31021 cgcccaatcc gtgaatacat gcgcaaattg tacatagagt caattttgtg aatgtaaaca
    31081 atactgtctt cgtccagtgc gccgaggtgg atagtttctt tggtcaggcg ggagatctca
    31141 cgcatctgga tatctgcgct acgaattaaa tcgacgtttt gtaacgcgcg agcgcccagt
    31201 tcaaacaatt tcagggtcag ggaatatttc tccgactccc cttcctgcgc cacataacct
    31261 aaggttttca tggtctgtaa aaagcgataa acggtgcttt ttgacatcat gacgcgctgc
    31321 gacagctcgg ttatccctat ttcgcgctct tcacccagcg cctgcaaaat gccaaaaact
    31381 tttagcacgg aagatacaga atcaggctgt ttatccagat ctgcgttagc catttatcac
    31441 ctcattgcga gtgttttata aaaatcagaa ctgtttttta ttataatttc gcaccagggt
    31501 ggtcgcaatc catcttttgc cggttagtta caattctgcg acatccaccg tgaatatcag
    31561 tgctagaatc atacccctgt tgattattca ccaaagatat aaaattccta tgccaaaagt
    31621 tcaggccgac ggcctgccat tgccccagcg atacggtgcg atattaacca ttgtgattgg
    31681 catttcgatg gctgtccttg acggcgcaat cgccaacgtc gccctgccaa caatcgccac
    31741 ggaccttcat gccacgccag ccagttccat ctgggtagtg aacgcctatc aaatcgccat
    31801 tgtcatctcc ctgctctcgt tttcgtttct gggcgatatg tttggctatc gacgtattta
    31861 taaatgcggt ctggtcgttt ttctgttgtc ttcactgttc tgcgcccttt ctgattcgct
    31921 gcaaatgctc acccttgcgc gtgtcataca aggtttcggc ggtgcagcgt tgatgagcgt
    31981 taataccgca cttatccgcc tgatctatcc acaacgtttt ctggggagag ggatgggcat
    32041 aaactcgttt attgttgccg tctcttctgc tgccgggccg acaattgctg cagcaatcct
    32101 ctccatcgca tcctggaaat ggttattttt aatcaacgta ccgttaggta ttatcgccct
    32161 gcttctggcg atgcgttttc tgccacccaa tggttctcgc gccagtaaac cccgtttcga
    32221 cctgcccagc gccgtgatga acgcgttaac cttcggcctg cttatcactg cgttgagtgg
    32281 tttcgctcag gggcaatcgc tgacgttaat tgctgcggaa ctggtggtaa tggttgttgt
    32341 tggtattttc tttattcgcc gccagctttc tcttcccgta ccgctgctac cggtggattt
    32401 actgcgtatc ccgctgtttt cactttctat ttgcacatct gtttgctctt tctgcgcaca
    32461 aatgctggca atggtttccc tgccctttta cctgcaaacc gtgctcgggc gtagtgaagt
    32521 cgaaacaggt ttacttctga caccgtggcc gttagcaacg atggtgatgg ctccgctggc
    32581 aggctatttg attgaacgcg tacatgcagg attgctgggt gctttagggt tgttcatcat
    32641 ggctgcgggg cttttttccc tggttctgct gcccgcgtca cctgcggata tcaatattat
    32701 ctggccgatg atcttatgtg gcgctggatt tggcttattc cagtcaccca ataaccacac
    32761 cattattacc tccgctccgc gcgaacgtag cggtggggcc agtggcatgt taggaacggc
    32821 tcgtctactg ggtcagagta gtggcgcggc gctggtggcg ctgatgctaa atcagttcgg
    32881 tgataatggt acgcacgttt cgctgatggc tgcggctatt ctggcagtga ttgctgcctg
    32941 tgtgagtggt ttacgtatca ctcagccacg atccagggca taaaaaaagc gcgtcgatca
    33001 ggacgcgctt tttagtattt acttcatatt acttcaggta ttcacccgta cgcagagctt
    33061 caattcgttt atccagcggc gggtgagtca tgaacaactc actgagcgat ttcgacttac
    33121 cgttaatgca gaaagccatc atgctggttg cttcttgcgg ttcatagctg gttttcaggc
    33181 gctgcagcgc ggcaatcatt ttctcgcgac caaccagttt tgccgaaccg gcatcagcat
    33241 ggaattcacg atgacgcgag aaccacatgg tgataatgct cgcaagaata ccaaacacca
    33301 gttccagaac cgttgcaacc gcaaagtaga tcagcgggtt gccgttgctc tcttcacctt
    33361 catcacgatt tccgcccata aaacccgcgg caagctgcgc cagaatacgg gaaataaaga
    33421 taacgaaggt gttcaccacg ccctgaatca gcgtcatggt gaccatatca ccattggcga
    33481 tgtggctgat ttcgtgagca attaccgcct cggcttcatc cgggctcatg ttctgcagca
    33541 aaccggtgct gacagcaacc agagaggcat cacggcgcgc accggttgca aaagcgttga
    33601 tgtccggcgc atggtagata gccacttgcg gcatagcgat ccctgcctga cgagcctggg
    33661 ttgctacagt attaaccagc caacgttccc tttcgttacg cggttgctcg atcacttccc
    33721 cgccaacaga tcgtaatgcc atccatttgg acatcagaag cgaaacgaag gaaccaccaa
    33781 aaccgaacag caaggccatg atcatcagcc cctgaacgct gctcgactgt atccctgtca
    33841 ggctcagtac cagcccgaaa acgaccatta cggccaggtt cgttagcagg aagagcgcga
    33901 ttcgcatcat aattttcttt ttacctcagt ttaacaaaac gcaatatgcg atacccacat
    33961 cgtatgggtt acgcgactat tttcaagtct ggatagtgcg taagtcacca gaaagacaca
    34021 actttacatt ttgtagcatc tgatttacgg catcttgccg ctgttaaaaa atcaggcaca
    34081 atttcttgtg cctgattgat attacttgac gggagcgggt tgttccgcgg gtctggcttt
    34141 ttcaagcttc gccagatcga gtgcgatatt caccgtctca tccagataag gatccggctc
    34201 ctggtaatct ttaggtagat catccagctt cttcaactcc ggtttacctt cgcgtttaaa
    34261 gcgttcgttc aaacgcgcca gacgcgtcgc atcatcttca ttattctctt tctcacgcac
    34321 agcgtagttc agagaaacga tattgcgctt gtccttcata gcgttgaagc gcgcgatatc
    34381 cttcatgatg ttctggaact caggatcttt cgcgatacgc gcattatgtt ccttcagcag
    34441 ctccggccca aaggccgtta aatcgcctga tttcacataa gtcgcggcat caatgctatc
    34501 ccacggcagc gcgttatctt cgaatttctc acccgtttcc gtttcttcat tacccgtcgg
    34561 catgatgatg tctggcgtta cgcctttacg ttgcgtactg ccgccgttaa cgcgatagaa
    34621 tttctggatc gtgtactgca cagaacccag cgctggccat tcaggacgta acatctgatc
    34681 gtaaatacgg ttcaatgaac ggtattgctg aacggtgcct ttaccaaacg tcggttcacc
    34741 cacaaccagc gcacgaccgt aatcctgcat tgccgcggca aagatttctg aagccgaagc
    34801 actgaaacgg tcaaccagta ccaccagcgg gcctttatag aacacctgcc cgtcggtatc
    34861 gctgtcttca cgaaccttgc cgttgttatc gcggacctga acaatgggac ccgcaggaat
    34921 aaacagacct gagagcgata ccgcttcggt cagcgcccca ccgccattgc tacgcaggtc
    34981 gatgatcaca ctgctgacat tctgtttttc cagtttctgc agttgaactt tgacatcgtc
    35041 tgtcaaaccc acatagaagc ccggaatatc cagcacgccg actttctctt taccgacggt
    35101 cttcaccgac attttaaccg cgcggtcttc gagacgaata cgttcacggg tcaatgttac
    35161 agtgcgggtc ttggtccctt taccagcagg taaaatttcc agacgaactt tactgccctt
    35221 cggcccttta attaaggcaa ccacatcatc aagacgccag ccaatcacgt caaccatcgg
    35281 cttgcctgtt tgaccaacac cgacaatttt gtcaccaacg ctgatagctt tactcttcgc
    35341 tgccggacca cctgccacca tcgaattgat aacggtgtag tcatcatcca tttgcagcac
    35401 tgcgccaata ccttccagcg acaaactcat ttcagtgttg aactgttcgg tattacgcgg
    35461 ggaaagatag ttggtatgcg ggtcgatttc acgcgcaaac gccgtcattg ccagcgagaa
    35521 aacatcttcg ctgttggttt gcgccagacg acgaatggca aatttgtagc ggcgtgtcag
    35581 ggtttcacga atttctttat ccgtttttcc tgccagcttc aggcttaact cgtcgaattt
    35641 gactttactg tcccacagcg cgttcaactc agcctcgttt ttcggccagg gcgctttgct
    35701 gcggtcaagg ttataagtgt cgttgccggt gaaatccatc ggcttttcca gtaccgacaa
    35761 agcgtactgg taacgctcaa aacggcgctt ttgcgccaga ttgtagagat cgtagaaaac
    35821 gtcgagtttg cctgaacgca gttcatcgcc taactcggtt ttctttttcg cgaactgttc
    35881 aacatcgctt gccagcagca cgttgtggct gtaatcgagc agattcaggt agcggtcaaa
    35941 gattttggcc gaaaatgcct gatcgaggtc gaactggcga taatgagaac gggtgaagcg
    36001 cgacgttacg cgctcactta ccgtcgcatg ctgcgtctct tcctttaata ccggaatttg
    36061 atcagcacgc gtgatatctt ctacagcgaa ggtctggcct gctattgcaa gcaggccagc
    36121 taacgcggta agcctaaaaa acatgttcat gcctggcccg gcctccgttt cagaacacca
    36181 ggtgttctgc gcgcacaatc aaagacatac ccgaattcag ctggacgcgg acgccgtctt
    36241 tggtgatttc taatacggtg gcatccatcg cgttttgacc cgctttcacc ttcagggctt
    36301 gtccgacagt cagagctgaa atgtcagaaa ccggggtgtg ctgttcttcg cgaggtgctt
    36361 ttactgtttt tggcgctttc tctaccggct tttgcgcacg aggtttacgt tcagcgcctt
    36421 ctttgcggcg tggcgtggtc ggacgtggct tgcgttcgcg gcgcggtgcg tcttctttct
    36481 caccagcagc tgcggcagct tcgcgttttt tcgcttgctg ttcagcacgc tgtgcctgaa
    36541 cacgcgcttt cgcttcttca agctgcttgc gagcatgctc tacatgttgc tcgtccagct
    36601 caccgcatgg gttgccgtca agatcgacac gcgttgcgcc gggtttaaca ccgtaaagat
    36661 aacgccagct cgaagtgtag agacgtaaag cggatcgtaa ttgcgttttg ctcaggttca
    36721 tttccccggc aacacgatcg accaaatcct gaaaaatacc gattttcagc ggacgcgctt
    36781 caccttccgc actgaaacag tggggaaaac gttcggccag aaacgcgatt acttctttac
    36841 tgctattcaa cttaggttga ttttccatga aatttcctga ttacaacgga cgtagccaac
    36901 aagcgcaggc atgaacaggc gtcattataa tgacgctacc agtaaatgct acgttatccg
    36961 ttgattatcc tgcgacgctc gcaaagaatt ttttgtaatc cgtcgttgca agcacttttt
    37021 caagctgtgc cacaagctga cgtaagcctt gctcgtcctc gtctgtaaag cgaccgaaga
    37081 cggtactatc gatgtcaaga acaccaataa tctgattttt caccaccagc ggcagaacaa
    37141 tttcagaatt actcgccgca tcacaggcaa tatgcccgtc aaacgcgtga acatcctcga
    37201 tacgctgcac ttgattgcgg gcaaccgcag tgccgcacac cccgcgcccg acgggtatcc
    37261 ggacacaggc aattttgccc tgaaatggtc ctagtaccag tgtatcgtcc tcaagcaaat
    37321 aaaaacctgc ccagtttacg tcagtgagac gctcgtataa caacgcactg gtgttcgcaa
    37381 gcgttgccag aaaactggtt tctcccgcca tcagcgcgtt aaagtcgcga tttaaatccg
    37441 cgtaaaattc tgttttgttc attatataat cacttggttg tcttacctgg aactgccagc
    37501 ctattaaaat aagcattaaa tgcgttaatg ctcaagatca ttccaatcat gggttaatat
    37561 taatgttaat tcttattaca tttggcacgt catggctctt aacacaccac aaattacgcc
    37621 gacaaaaaag ataacagtga gggcaatcgg cgaggaactg ccgcgtggtg attaccaacg
    37681 ttgcccgcaa tgtgacatgc tgtttagcct gcccgagata aattctcatc agagtgccta
    37741 ttgtccgcgc tgtcaggcaa aaattcgcga cgggcgcgac tggtcgctaa cgcgcctggc
    37801 ggcaatggct ttcaccatgc tgttgctgat gccgtttgcc tggggcgaac cgctgttgca
    37861 tatctggctg ttaggtattc gtatcgacgc caacgtcatg caaggcatct ggcaaatgac
    37921 caaacagggc gatacgataa cgggggcgat ggtctttttc tgcgttattg gtgcccccct
    37981 cattctggtg tcctccatag catatttatg gtttggtaac cgactgggaa tgaatttacg
    38041 tccggtactg ctgatgcttg agcgacttaa agagtgggta atgctggata tctacctggt
    38101 cggcattggc gttgcttcta taaaggtaca ggattatgcc catatccagg cgggtgtcgg
    38161 cttgttctct tttgtggcgt tggtgatttt aacgacggtg acgttgtcac atcttaatgt
    38221 cgaggagctg tgggagcgat tttatccgca gcgccccgct acgcgtaggg acgagaaact
    38281 tcgtgtctgt cttgggtgcc attttaccgg ctatcctgat cagcgtggtc gctgcccgcg
    38341 ttgccatatc ccgctacgcc tgcgtcgccg tcatagtttg caaaaatgct gggcggcgct
    38401 gttagcgtca atcgttttgt tgttacctgc caacctgttg cctatttcta tcatttatct
    38461 gaacggagga cggcaggaag atacaattct ttccggaatt atgtcgctgg caagtagcaa
    38521 cattgcggtt gcgggaatcg tgtttatcgc cagtattctg gtaccgttta ctaaagtgat
    38581 cgtcatgttc actttactgt tgagcattca ttttaaatgc cagcaaggtt tacgcacacg
    38641 cattctgtta ctgcggatgg tgacctggat tggtcgctgg tcgatgctcg acctgtttgt
    38701 catatcttta accatgtcgc tgattaatcg cgatcagatc ctcgctttta ctatgggacc
    38761 ggctgcgttt tatttcggcg cagcggtaat tttgactatt cttgctgtgg aatggctgga
    38821 cagccgctta ctttgggatg cacatgagtc aggaaacgcc cgcttcgacg actgaagcgc
    38881 agattaaaaa taaacgccgt atctcacctt tctggctgct gccgttcatc gcgctaatga
    38941 ttgccggttg gctgatttgg gacagttatc aggaccgggg taataccgtc accatcgact
    39001 ttatgtcggc ggatggtatt gtcccgggcc gtacgcctgt tcgttatcag ggcgttgaag
    39061 tcggaacagt gcaggatatc agcctcagcg acgatcttcg taagattgaa gtcaaggtca
    39121 gcatcaagtc cgatatgaaa gatgcgctgc gcgaagagac tcagttctgg ctggtgacgc
    39181 caaaagcatc gttggcaggt gtctccgggc tggacgccct cgtcggtggt aactatatcg
    39241 gcatgatgcc aggtaaaggt aaagagcagg atcactttgt cgcactcgat acccaaccga
    39301 aatatcgact ggacaatggc gatctgatga tccacctgca agcccccgat ctcggttcgc
    39361 tgaacagcgg ttcattggtc tatttccgca agatccctgt gggaaaagtc tacgactatg
    39421 ccatcaatcc caacaagcaa ggcgtggtga ttgatgtcct gatcgagcgg cgttttaccg
    39481 atctggtgaa aaaaggtagc cgtttctgga acgtttccgg cgttgatgcc aatgtcagta
    39541 tcagtggcgc gaaggtgaaa ctggaaagtc tggcggcact ggttaacggt gcgattgcct
    39601 tcgattcacc tgaagagtcg aaacctgccg aggcggaaga tacctttggt ctgtatgaag
    39661 atctggccca cagccagcgt ggcgtaataa taaaactgga actgccgagt ggggcaggat
    39721 taaccgccga ctcgacgcca ttaatgtatc agggactgga agtcggacag ctaactaaac
    39781 tggatttaaa tcctggtggt aaagtcactg gggaaatgac agttgatccc agcgtcgtta
    39841 ccctgcttcg tgaaaatacc cgcatcgaat tacgtaatcc gaaattatcc cttagcgatg
    39901 ccaatctcag cgccctgctg actggcaaaa ccttcgagtt ggtacccggc gatggcgagc
    39961 cacgcaaaga gttcgttgtt gtgccaggcg aaaaagcact gctgcatgaa cctgatgttc
    40021 tgacgctgac cctgaccgcg ccggaaagtt acggtattga tgctggtcag ccgctcattc
    40081 ttcacggcgt gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct
    40141 ttaccgtcgc catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg
    40201 tcaacagccg tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg
    40261 cctcagaatg gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag
    40321 ccagctatcc actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac
    40381 ccaccacaac cgtgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag
    40441 tgctctaccg taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt
    40501 ttgatatcga tctgcatatt aagccggagt atcgcaacct tctgaccagc aatagcgtgt
    40561 tctgggcaga aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat
    40621 ccccgctctc cagagcatta aaaggggcta ttagcttcga taacctcagt ggtgccagcg
    40681 ccagtcagcg taaaggcgac aaacgtattc tgtatgcttc cgaaacagcg gcccgtgcgg
    40741 ttggtgggca gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa
    40801 ttcgctatct cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcgcgca
    40861 atgaagtaca ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg
    40921 gtacgcgctt ctcagtggtc acaccgcaaa tttcggcagc tggcgttgag catcttgata
    40981 ctatcctcca gccgtatatc aacgtcgaac caggccgggg caatcctcgc cgcgactttg
    41041 aattacaaga ggccaccatt actgattcgc gttacctgga tggcttaagc attattgttg
    41101 aagcgccgga agccggttcg ttaggtattg gtacgcctgt gctgttccgt ggtctggaag
    41161 tcggtacggt tacaggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc
    41221 gcatcagtaa acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg
    41281 gttacagtct ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccagt
    41341 ttatccgtgg cggcatcgcc ttcgccacgc ctccgggtac gccactggca ccgaaagccc
    41401 aggaaggcaa acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa
    41461 ctgcgcttcc caaataatgc ccactgctcc ggcgtgcctg cgccggagcg tttatgctaa
    41521 actgcgcgcc tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc
    41581 ccggacgcct ttctgacaca aatgcgcgaa gcgatgcctt cgacgctctc atttgatgat
    41641 tttcttgccg cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc
    41701 tccgttgctg atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg
    41761 tggtgtgaag aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt
    41821 accgccgagc atttaagcgg cctgttttat attcaggaag ccagttcaat gttgcccgtt
    41881 gccgccttgt ttgctgacga taatgcacca cagcgggtga tggatgtcgc agctgcgcca
    41941 ggctccaaaa cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat
    42001 gagttttccg ccagtcgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt
    42061 aatgttgcgc tcacacattt tgatggccgc gtgtttggtg cggcagtgcc agaaatgttc
    42121 gatgccattt tgctggacgc tccctgctct ggcgaaggcg tggtgcgtaa agatcccgat
    42181 gcgctaaaaa actggtcacc agaaagcaat caggaaatcg cagctacaca acgggagctt
    42241 atcgacagcg cctttcatgc attacgtcct ggtggtacgc tggtttactc gacctgtacc
    42301 ttaaaccagg aagaaaacga agccgtttgc ctgtggctga aagagactta ccccgacgca
    42361 gtagagtttt taccacttgg cgatctcttc cctggtgcaa acaaagcgct gaccgaagaa
    42421 ggctttttgc atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg
    42481 cgtaaaactc aggcgatccc cgccttaccc gcccccaaat acaaagtcgg taatttcccg
    42541 ttcagcccgg tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc
    42601 ttaaactggg atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg
    42661 gtgggcattg aagccctgat cggtaaagtc cgattttctc gcttggggat taaacttgcc
    42721 gaaacgcaca acaaaggtta tcgctggcag catgaagcag ttattgccct tgccaccccc
    42781 gacaatgtga acgcttttga actgacaccg caggaagcgg aggagtggta tcgcgggcgc
    42841 gatgtttacc cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcaa
    42901 ccgattggtt tagccaaacg gattggttcg cgattgaaaa acagctatcc gcgtgaactg
    42961 gtgcgcgatg ggaaactttt taccggtaac gcctgacagc gcacaaaaaa agcgcacttt
    43021 ttgactggca cattcggctg cctcaactag gctgaaaaat ggtgcgatcg gactggtcgt
    43081 accacaaccg gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct
    43141 tttgaaatcg acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt
    43201 ccttgtaaat ctgaccccga tttatgtatg caactcgatg cctgggatgc tgaaaccagt
    43261 atccctgccc tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa
    43321 tctgatgcct ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt
    43381 atttgtcctg gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac
    43441 acttcgggga tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg
    43501 aagatattgt tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc
    43561 aacggatccg ccatggcggt tgttttccga ttgaggattt tatagatggt ttctggcgac
    43621 cagcacagga gtatggtgat ttttaattat tgcaattgca caagagtcag ttcgccccca
    43681 aagacagcac cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt
    43741 gtatgaccaa accagaaatg atcagcacct gtaattccct gccctttttg gcgttcacct
    43801 aatcgcgagc ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcataaaca
    43861 tcatctggat aatcggcatg agcaataaca tgtttgccgg tacgactgtg tacttcaaga
    43921 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
    43981 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctgggatgcc
    44041 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
    44101 agtaactgca ggcaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
    44161 agtaaatctc gccacggatc aaaaccacaa tgccataatt tgcggcgcaa ctgctcaaga
    44221 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
    44281 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
    44341 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
    44401 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acacgattcc
    44461 tgttttcgtc aacaaacaac aaaatctttt agaattaatg tgttaaataa
//
LOCUS       DAZFYC010000027        43410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFYC010000027 DAZFYC010000000
VERSION     DAZFYC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.027"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..136)
                     /locus_tag="ACHKW5_001677"
     CDS             complement(<1..136)
                     /locus_tag="ACHKW5_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFO7511089.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     Q"
     gene            349..1140
                     /locus_tag="ACHKW5_001678"
     CDS             349..1140
                     /locus_tag="ACHKW5_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO7511090.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1150..1992
                     /locus_tag="ACHKW5_001679"
     CDS             1150..1992
                     /locus_tag="ACHKW5_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7511091.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2002..2778
                     /locus_tag="ACHKW5_001680"
     CDS             2002..2778
                     /locus_tag="ACHKW5_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO7511092.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            2788..4329
                     /locus_tag="ACHKW5_001681"
     CDS             2788..4329
                     /locus_tag="ACHKW5_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7511093.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4326..6407
                     /locus_tag="ACHKW5_001682"
     CDS             4326..6407
                     /locus_tag="ACHKW5_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO7511094.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYEWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6428..6904
                     /locus_tag="ACHKW5_001683"
     CDS             6428..6904
                     /locus_tag="ACHKW5_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO7511095.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            6947..8248
                     /locus_tag="ACHKW5_001684"
     CDS             6947..8248
                     /locus_tag="ACHKW5_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO7511096.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            8301..9272
                     /locus_tag="ACHKW5_001685"
     CDS             8301..9272
                     /locus_tag="ACHKW5_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO7511097.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(9384..9833)
                     /gene="rplI"
                     /locus_tag="ACHKW5_001686"
     CDS             complement(9384..9833)
                     /gene="rplI"
                     /locus_tag="ACHKW5_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO7511098.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(9875..10102)
                     /gene="rpsR"
                     /locus_tag="ACHKW5_001687"
     CDS             complement(9875..10102)
                     /gene="rpsR"
                     /locus_tag="ACHKW5_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO7511099.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(10107..10421)
                     /gene="priB"
                     /locus_tag="ACHKW5_001688"
     CDS             complement(10107..10421)
                     /gene="priB"
                     /locus_tag="ACHKW5_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO7511100.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(10428..10823)
                     /gene="rpsF"
                     /locus_tag="ACHKW5_001689"
     CDS             complement(10428..10823)
                     /gene="rpsF"
                     /locus_tag="ACHKW5_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO7511101.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            11150..11425
                     /gene="yjfY"
                     /locus_tag="ACHKW5_001690"
     CDS             11150..11425
                     /gene="yjfY"
                     /locus_tag="ACHKW5_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO7511102.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDMQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(11554..12240)
                     /gene="ulaF"
                     /locus_tag="ACHKW5_001691"
     CDS             complement(11554..12240)
                     /gene="ulaF"
                     /locus_tag="ACHKW5_001691"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO7511103.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12240..13094)
                     /gene="ulaE"
                     /locus_tag="ACHKW5_001692"
     CDS             complement(12240..13094)
                     /gene="ulaE"
                     /locus_tag="ACHKW5_001692"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO7511104.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(13104..13754)
                     /gene="ulaD"
                     /locus_tag="ACHKW5_001693"
     CDS             complement(13104..13754)
                     /gene="ulaD"
                     /locus_tag="ACHKW5_001693"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7511105.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(13768..14232)
                     /gene="ulaC"
                     /locus_tag="ACHKW5_001694"
     CDS             complement(13768..14232)
                     /gene="ulaC"
                     /locus_tag="ACHKW5_001694"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO7511106.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(14242..14547)
                     /gene="ulaB"
                     /locus_tag="ACHKW5_001695"
     CDS             complement(14242..14547)
                     /gene="ulaB"
                     /locus_tag="ACHKW5_001695"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO7511107.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSKIDHTVNSCAVGEY
                     KSELSNADIIIASTHIAGEISVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(14563..15960)
                     /gene="ulaA"
                     /locus_tag="ACHKW5_001696"
     CDS             complement(14563..15960)
                     /gene="ulaA"
                     /locus_tag="ACHKW5_001696"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7511108.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYN
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            16315..17379
                     /gene="ulaG"
                     /locus_tag="ACHKW5_001697"
     CDS             16315..17379
                     /gene="ulaG"
                     /locus_tag="ACHKW5_001697"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO7511109.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVASGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            17487..18242
                     /gene="ulaR"
                     /locus_tag="ACHKW5_001698"
     CDS             17487..18242
                     /gene="ulaR"
                     /locus_tag="ACHKW5_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO7511110.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(18239..18988)
                     /gene="yjfP"
                     /locus_tag="ACHKW5_001699"
     CDS             complement(18239..18988)
                     /gene="yjfP"
                     /locus_tag="ACHKW5_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7511111.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            19170..19499
                     /gene="bsmA"
                     /locus_tag="ACHKW5_001700"
     CDS             19170..19499
                     /gene="bsmA"
                     /locus_tag="ACHKW5_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO7511112.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            19648..19923
                     /gene="yjfN"
                     /locus_tag="ACHKW5_001701"
     CDS             19648..19923
                     /gene="yjfN"
                     /locus_tag="ACHKW5_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO7511113.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(20040..21665)
                     /gene="aidB"
                     /locus_tag="ACHKW5_001702"
     CDS             complement(20040..21665)
                     /gene="aidB"
                     /locus_tag="ACHKW5_001702"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO7511114.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(21749..22912)
                     /gene="yjfC"
                     /locus_tag="ACHKW5_001703"
     CDS             complement(21749..22912)
                     /gene="yjfC"
                     /locus_tag="ACHKW5_001703"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7511115.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(22915..23553)
                     /gene="yjfM"
                     /locus_tag="ACHKW5_001704"
     CDS             complement(22915..23553)
                     /gene="yjfM"
                     /locus_tag="ACHKW5_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO7511116.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(23563..23962)
                     /gene="yjfL"
                     /locus_tag="ACHKW5_001705"
                     /pseudo
     CDS             complement(23563..23962)
                     /gene="yjfL"
                     /locus_tag="ACHKW5_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
     gene            complement(23980..24639)
                     /gene="yjfK"
                     /locus_tag="ACHKW5_001706"
     CDS             complement(23980..24639)
                     /gene="yjfK"
                     /locus_tag="ACHKW5_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO7511117.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(24690..25388)
                     /gene="yjfJ"
                     /locus_tag="ACHKW5_001707"
     CDS             complement(24690..25388)
                     /gene="yjfJ"
                     /locus_tag="ACHKW5_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO7511118.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(25407..25808)
                     /gene="yjfI"
                     /locus_tag="ACHKW5_001708"
     CDS             complement(25407..25808)
                     /gene="yjfI"
                     /locus_tag="ACHKW5_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO7511119.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSQVDNALDLAEITEEYSH"
     gene            complement(25935..26666)
                     /gene="rlmB"
                     /locus_tag="ACHKW5_001709"
     CDS             complement(25935..26666)
                     /gene="rlmB"
                     /locus_tag="ACHKW5_001709"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO7511120.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(26846..29287)
                     /gene="rnr"
                     /locus_tag="ACHKW5_001710"
     CDS             complement(26846..29287)
                     /gene="rnr"
                     /locus_tag="ACHKW5_001710"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO7511121.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLSKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(29326..29751)
                     /gene="nsrR"
                     /locus_tag="ACHKW5_001711"
     CDS             complement(29326..29751)
                     /gene="nsrR"
                     /locus_tag="ACHKW5_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO7511122.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(29956..31254)
                     /gene="purA"
                     /locus_tag="ACHKW5_001712"
     CDS             complement(29956..31254)
                     /gene="purA"
                     /locus_tag="ACHKW5_001712"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO7511123.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(31358..31555)
                     /gene="yjeT"
                     /locus_tag="ACHKW5_001713"
     CDS             complement(31358..31555)
                     /gene="yjeT"
                     /locus_tag="ACHKW5_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO7511124.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(31637..32641)
                     /gene="hflC"
                     /locus_tag="ACHKW5_001714"
     CDS             complement(31637..32641)
                     /gene="hflC"
                     /locus_tag="ACHKW5_001714"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO7511125.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(32644..33903)
                     /gene="hflK"
                     /locus_tag="ACHKW5_001715"
     CDS             complement(32644..33903)
                     /gene="hflK"
                     /locus_tag="ACHKW5_001715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO7511126.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(33989..35269)
                     /gene="hflX"
                     /locus_tag="ACHKW5_001716"
     CDS             complement(33989..35269)
                     /gene="hflX"
                     /locus_tag="ACHKW5_001716"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO7511127.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(35346..35654)
                     /gene="hfq"
                     /locus_tag="ACHKW5_001717"
     CDS             complement(35346..35654)
                     /gene="hfq"
                     /locus_tag="ACHKW5_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO7511128.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(35740..36690)
                     /gene="miaA"
                     /locus_tag="ACHKW5_001718"
     CDS             complement(35740..36690)
                     /gene="miaA"
                     /locus_tag="ACHKW5_001718"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO7511129.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(36683..38530)
                     /gene="mutL"
                     /locus_tag="ACHKW5_001719"
     CDS             complement(36683..38530)
                     /gene="mutL"
                     /locus_tag="ACHKW5_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO7511130.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(38540..39877)
                     /gene="amiB"
                     /locus_tag="ACHKW5_001720"
     CDS             complement(38540..39877)
                     /gene="amiB"
                     /locus_tag="ACHKW5_001720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO7511131.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(39896..40357)
                     /gene="tsaE"
                     /locus_tag="ACHKW5_001721"
     CDS             complement(39896..40357)
                     /gene="tsaE"
                     /locus_tag="ACHKW5_001721"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO7511132.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(40329..41876)
                     /gene="nnr"
                     /locus_tag="ACHKW5_001722"
     CDS             complement(40329..41876)
                     /gene="nnr"
                     /locus_tag="ACHKW5_001722"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO7511133.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            41875..43014
                     /gene="queG"
                     /locus_tag="ACHKW5_001723"
     CDS             41875..43014
                     /gene="queG"
                     /locus_tag="ACHKW5_001723"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO7511134.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(42997..43050)
                     /locus_tag="ACHKW5_001724"
     CDS             complement(42997..43050)
                     /locus_tag="ACHKW5_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511135.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(43284..43359)
                     /locus_tag="ACHKW5_001725"
     tRNA            complement(43284..43359)
                     /locus_tag="ACHKW5_001725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43324..43326),aa:Gly,seq:gcc)
BASE COUNT        10437 a        11652 c        10788 g        10533 t
ORIGIN      
        1 tctgtttact acaccccgca gccttaacaa tattatcgac cctggcccct tcataaccat
       61 attcggcaaa ttcattaacc gcgcataaca gcagcttttc cttcaggctc tgtacgttgc
      121 tggtatcaag atacataacg cttccccacg acctcttcaa gataactatc ttaacccgat
      181 taattagaag ctccagtaaa aaggataaaa acttgtactt tcatcaagtt atcaaatgtc
      241 ctgtgatcac tatcgcatat caaccaacca gatggttgat ctttcttgcg gcacgtcgca
      301 agctaattgt cacttagaaa acaatattca aaaaaagaga gctattgtat gaaatccact
      361 cgtcctgttg ccgtgattac cggagccgct cgtggtattg gaaagggctg tgcgctggag
      421 cttgctcgtg gcggatttaa cctgctgatt aacgatcttc ccgatgccga cagcgtagaa
      481 aaactgcata tcacacaaca agaatgtata gctgaaggcg tggaagtgat ttgcttccct
      541 gccgatgtcg gcgatctctc cttgcatgaa gagatgctcg acgcggcgca aaaccagtgg
      601 ggacgtctgg actgcctgct caacaacgca ggcatttcag tgaaaaaacg cggtgacctt
      661 ctcgacctcg aaccagacag cttcgaccaa aatattgcca ttaacacccg cgcgccgttc
      721 ttcctcgctc aggcattcag taaacgtctg ctcgcacagc cgaaaccgga agcagaactg
      781 ccgcaccgct cgattatttt cgtcagttcc atcaacgcca tcatgctggc gatgaatcgc
      841 ggcgaatata ccattgccaa aaccgccgtt tctgccgctg ctcgtctgtt tgctgctcgc
      901 ctttgtaatg aacaaattgg cgtttatgaa gtgcgccccg gtctcatcaa aaccgatatg
      961 actattccgg caacggctta ttacgacgaa ctgattgcca aaggtctggt gccgtgggga
     1021 cgttggggtt atccggctga tattgcttcc actgtccgcg cgatggcgga aggtaaactg
     1081 atttacacct gtggtcaggc ggtcgccatc gacggcggct taagtatgcc gcgcttctga
     1141 ggctgaatca tgagagatct gttacaacgc ccggatttat tcagtatcaa taccgcaaca
     1201 ctgggctata aaacgccgct acccgccatt attgatgcct gcgccgcgcg tggtattggc
     1261 gcgattgctc cctggcgcag ggagttgcaa ggtgaagatc tccagcaaat cgcccaccag
     1321 cttgccgcca gtaacatgag cgtttccggt ttgtgccgta gcacttacta caccgcgccg
     1381 acactggcgg aacgcaaact ggcgattgat gataaccgcc gggcgctgga cgacgcagcg
     1441 gtgcttaacg ccgcctgtta tatgcaggtg gttggcggtc tgccaacggg cacaaaagat
     1501 ctgtacgaag cgcgggaaca agtaaaacaa gggattcgcc agttgctgcc acattcgaaa
     1561 gatgtcggcg tgccaattgc gctggagcca ctgcacccga tgacagctgc cgatcgctct
     1621 tgcctgtgca cgttacgcca ggcgctggac tggtgtgatg aactcgatcc cgatggtgag
     1681 tttggcctcg gcgtggcggt ggatgtttat cacgtctggt gggatccgga tctcgccagt
     1741 cagatcctgc gtgccggaaa acgcatcctc gcgtttcatg tttccgactg gttagtgccg
     1801 accactgatc tggtcaatga ccgtggtatg ccgggagatg gcgtcatcaa tattccgtca
     1861 attcgtcggc tggttgaaaa cgccggattt aatggtgcta ttgaactgga aatattctcg
     1921 ccttactggt ggcagaaaga tattaacagc acgctggata ttagcgtcga tcgcatcgcg
     1981 cattattgtt aaggaatgat catgagtgac caacccgttc tgttcagcag agtggcggct
     2041 tcctgccgcc tgacattgaa ccgtgaagat aaatgtcatg ctattaacga agagatgatc
     2101 gaatctctcg accattattt aaatgaaatt gaaaacgaca ccacattacg attagtcgaa
     2161 ttaacggcaa ccggcgataa attcttttgt gcgggcggcg atattaaatc gtggtcggcc
     2221 tattcgccat tagatatggg cagaaaatgg attaaacgcg gtaatgacgt ttttaatcgc
     2281 ctgcgaaact taccccaatt aaccgttgcg aatctcaacg gtcataccat cggcggtggt
     2341 attgaactgg ccttgtgctg cgatattcgt attgcccgcc ccggcgcgaa attctctaac
     2401 ccggaagtga tgctcggcat ggtgcctggc tggatgggca ttgaacgtgt gcttaatcag
     2461 gttggtccgg tggtcggtcg ccagatgctg atgctgggta aacgcctgac ggcgcaggaa
     2521 gcccaggcgg cgaatttaat tgatgaagtg gtagaaaaag aacaggtgga aagctggatg
     2581 gcgaaccagt tagcgcagct ggaaaaatgc ggtccggtgg cgcttgcaca tattaagcaa
     2641 ctgattctgg cactggaaaa taaacatgcc gattacccgc accagctact ggccggatta
     2701 atgtccgcca ctcaggattg ccagcaggcg acgcgcgcct ttgccgaaaa aagtagcgta
     2761 agcttccata atcaataagg aaattgtatg cgtaagataa caaccgccga ggcactggcg
     2821 gcgcaaattc aggacggtgc gactattgct attagcggta acggcggcgg tatggtggaa
     2881 gcggaccata ttctggctgc tattgaagcg cgtttcctgc aaaccggaca ccctcgcgac
     2941 ctgacgctaa tccactcgct gggtattggc gatcgcgact gcaaaggcac taatcgtttt
     3001 gcgcacgcag agatgctcaa acgcattatt gccgggcact ttacctggtc gcccaaaatg
     3061 caggcgctgg taaaaaataa cacgattgaa gcctactgct ttcctggtgg cgtgattcag
     3121 gcactgctac gggagatcgg cgccggacgt ccggggcttt ttacccacgt tgggctggga
     3181 tcgtttgttg atccacgcaa tggcggcggt aagtcgaatg aatgcactac cgacgacctg
     3241 gtagaactga ttgaaatcga tggtgaaacc aaacttcgtt atcgcccttt caaagtggat
     3301 tacgcgattt tgcgtggcac ttatgccgat cctcgaggca acgtcagcct tgaagaagaa
     3361 gcgattgata tggatagcta ttccatggcg ctggcagcac acaacagcgg cggcaaagtg
     3421 ttcgtacagg tacgcgatgt gctggaagct ggcgcgattg aaccacgccg ggtcaaatta
     3481 ccgggcattc tggttgatgg catcgttgaa caccgcgaac aaccgcaaac ctatcttggc
     3541 ggttacgacc tgaccattag cggtcaacat cgccgtctga gttctaacga cgctattgaa
     3601 ctggttagtc atccggtgcg ccgcctgatt gcccgtcggg cagcacgcga actggtggct
     3661 ggcgcttcaa ccaactttgg ctttggtatt cctggcggta ttccaggcgt agcgctgcgc
     3721 gaaggcgttc cttaccaaag tttatggctg agcgtcgagc aaggcgtaca taacggcatg
     3781 atgcttgatg atgcgttttt cggctgcgcc cgcaacgccg atgccattat tccatcactc
     3841 gatcaatttg agttctacag tggcggaggg atcgatatca ccttcctcgg catgggagag
     3901 atggatcagt acggtaacgt caacgtctcc catctcaatg gcaatctcat tggtcccggc
     3961 ggttttctcg aaattgcgca aaacgcccgt aaagtggtgt tctgcggcac gttcgacgcc
     4021 aaaggtagca agattgatgt aacgccagat ggcttgcata tcacccagtc aggtcaaatc
     4081 cctaaactgg ttacccaggt ggaaaaaatc acttttagcg ccgcctacgc acagcaaagt
     4141 ggtcaggaag tgttgtatat cactgaacgc gcagtattcc agttaacggc agaaggcgtt
     4201 gaattaattg aaatcgcgcc gggtgtggag attgagcgcg acattctgcc gtatatggct
     4261 ttccgtccaa ttatcaagca cccacgcctg atggaaagta gcctgtttac gccgatggag
     4321 gatgcatgag tcagcttgat gacaccatcc ttgatgcgct gacgcacgtt actttcccaa
     4381 agggttttgc acaggcagag cccgcatggg ttgtcacggt agacggtgtt gattacccac
     4441 tctggcaaac agatgccctg gtagtcggca gtggtgcagc aggattacgt gcggcagttg
     4501 aacttaaacg ccgccagcaa aatgtgctga tcgctaccgc cgggttatat atggggacgt
     4561 cggcatgttc tggttccgat aaacagacac tatttaccgc cgctaccgcg ggcaacggcg
     4621 acaacttcac caaactggca gaggcactgg cgagcggtgg ggcgatggat cacgacaccg
     4681 cttatgtcga agcggtaggt tctctgcaca ctctaggcgg gttgcaatat cttggtctgg
     4741 aattaccgga agatcgctat ggcgcgattc ttcgttatca aaccgaccat gacgaagccg
     4801 ggcgtgcaac ctcgtgtggg ccgcgtacct caaggttaat ggtgaaagtg ctgttggaag
     4861 aagtacagcg cctcgccatt ccagtgttga ccagtgcaac agtgattaaa ctgctgcatc
     4921 agcgtgacga aaacggcgaa gaccgcgtgg caggggcaat cctcgcgacc ggtcatcgcg
     4981 cccataaccc atgggggctg gcaattgtga ctgcgcccaa tgtggtactg gcaacgggcg
     5041 gaccgggcga gctttatcgc gacagtgttt atccacataa atgcttcggc tcgctggggc
     5101 tggcgctgga ggaaggtctg acgctaacca atctgaccga aagccagttt ggtataggca
     5161 cgccgcgtag cacgtttccg tggaatttat ccggcaccta tgtgcaggtg atcccgtata
     5221 tctattccgt ggatgctgag ggtaacgagt ataacttcct cgcggattac tatcgcacca
     5281 cccaggagct ggcttcaaac attttccgta aaggctatga gtggccgttc cacgccactc
     5341 gggtgatgga ttttggctcc agcttgttag atatggcagt agcgcaagag cagcaatcag
     5401 ggcgtcaggt atttatggat ttcaatcgca atcctgaagc ggtgccgggt gacctgccgt
     5461 tctcattaga tcgactggac gacgacgttc gcgcgtatct ggaaaataac gacgctctgg
     5521 caccatcgcc catcgaacgg ctgcaacgaa tgaatccgct gtctatctcg ctgtataaga
     5581 tgcacggtta cgatctcacc acacagcctt tgcagtttgc catgaataat cagcatatga
     5641 atggcggcat tgaagtggat atctggggac aaacctccct gcccggctgt ttcgccgtgg
     5701 gggaagttgc tggcacacac ggcgtcactc gccctggtgg tgcagcattg aatgccgggc
     5761 aggtttttgc tgttcgtctg gcacgtttta ttggttgcac gcaaaagcgg aatattgatg
     5821 gagatatagc acagctggta gctcctacac tggcttctat aagagagatc attactcagg
     5881 cgcacgataa cgggaccggg atgccgttgt cggttgtgag agaaaaaatt caggcacgaa
     5941 tgtctgacca tgcgggattt atttgccatg ccgataaagt ccgacgcgcc actcgcgatg
     6001 ccctgctatt gagcgaattt gtccaacggc atggattggc tatcaaacat gtgggtgaag
     6061 ttgccgagct gtttatgtgg cggcatatgg cactgacttc tgccgccgta ttaacgcaat
     6121 taacgcatta tattgacgca ggtggtggta gtcggggagc acgtattatt ctcgacaggg
     6181 atggaaattc tattccgcaa acacgaaatg gtttttgtga tgcctggcgc tttcgttcag
     6241 agcgtactga ggataaaaaa gataaactac ttattcatta ttgcaacggt atctttcacg
     6301 tcagagaaac acctgttcgt gagtttccta ttatccgagg tatttggttt gaaaaaaact
     6361 ggcctggttt tcttaatggg acaatttacc agccacagga tgaatgatat tgcaaaggtt
     6421 aatcactatg cgaaatataa ttagatctat taatgctcta cttgcagcat tgaacattac
     6481 cattttggcg atcatcgtgg catgtgtgac gtggcaggtg gctgcacgat ttatatttac
     6541 tagtcccagt atttttacag atgaactcag ccgtttgctc tttatttgtc ttggactttt
     6601 tggtggggca tacaccgcag ggcaaaaccg gcatctggcg attgacctgc tacctatgat
     6661 gctgaaagga aaagcccgac gtcatttatt tttgtgtatt cagattatcg ttattatctt
     6721 cgcaaccatc attatggtat atggtggtgg gctattaacg atggatacat tcgattcagg
     6781 tcagacctcg cccgctttag gctggcaaat gggatatatc tatatgagca tcccgattag
     6841 tggtgtactt attattatat acaccataga tatggtttta accgaactta aacaaccact
     6901 ctaaagataa caaacaacaa ccaaccatag taggaatgga gaaattatgg attactggtt
     6961 accgattatt gtgttatttg gtgccttctt ttttatgttg gcattaggtg tacctattgt
     7021 ttatgccatt ggtttatcga cactggcatc aatttccaca caactcgatt tcaactcagc
     7081 attgagtgtg gtttcccaaa agttagcctc cgggttagat agttttacgt tgttggcaat
     7141 cccatttttt atcttgtctg gcaacattat gaaccatgga ggaatagcgc gacgattaat
     7201 caactttgcc cgtattctcg gcggtcgtct gccaggctca cttgcccact gtaatattct
     7261 cgccaatatg ctgttcgggg caatttctgg ttcagccgtg gcttcggcgg ctgcgatggg
     7321 cggcgtcatg catccgcaac aggttaaaga gggttacgac ccggcattca gtactgcggt
     7381 taacgttgcc tctgccccga cgggtttgct tattccacca agtaatacgt taatcgtcta
     7441 ttcactggtc agtggtggta cttctattgc cgcactgttc ctggcgggtt atgttccggg
     7501 aattttgctg ggtctggcac tgatggtaat agccggtatt atcgcggtac gtcgcggtta
     7561 tccaaagcca gaacgcccga ctttacgcca ggctggcgtt gcgatatgga tggctatccc
     7621 cagtattttc ctgattattt taattatggg gggtgtatta agtggtattt ttaccccgac
     7681 agaggcctcc gcaattgcag taatttatac ccttttcctt gcactggttc tttatcgtga
     7741 aatctcagtt aaagatttac ctaaaatatt tctggaatcc gttattacca ctgcaattgt
     7801 cttgttatta attggttctt cgatgggtat gtcatgggca atgtcaaacg ctgacgttcc
     7861 cttcctgatt ctcgatttgc ttaacactat ttctgacaac ccaataataa tcctgcttat
     7921 tatcaatatt atcctgttga ttatcggcac ttttatggat atgacaccgg cggtactgat
     7981 ttttacgccg atatttttac cggttgtcac cgaactgggc atggacccga ttcactttgg
     8041 tattgtcatg gtactgaaca tgtgtatcgg tatttgtact ccgcctgtag gcagtgtgtt
     8101 atttgtcggt tgtagtgtat ctaaattacc gattaataaa attatcaaac ctatgctgcc
     8161 attctatgcc gtgatggtat tggtgctggc aatggtgacc tatattccgc aaatcagtat
     8221 ggcattgcca agagcactgg gctactgatg cgcattcact ggcggcacct gccgccatat
     8281 ttattagaaa aggaacatct atgaaaataa aagtatccgc tggcattatc ggtgctgttc
     8341 ttatgttatc cgcaagccag tcctgggcag tgacattaaa actgagtcat aatcaggata
     8401 agtctcatcc tgttcataaa gcgatggagt tctttgcgaa aaagagcaaa gagtactcta
     8461 acggtgatat tactattcgt atttatccaa atggaacatt gggtactcaa cgagaaacaa
     8521 tggagctgat tcgttctggc gctattcccc tggtaaaaac caatgcggca gaaatggaag
     8581 catttgaaaa ttcctataaa ttatttagcc tgccttattt gttccgcgat cgtgatcatt
     8641 attatcaggt catgcagggc gatatcggga gaaaaatcct cgactcaacg aaaagcaaag
     8701 gttatttcgg gctgactttt tatgatggag gcgcccgcag tttctatggc aataaaccag
     8761 tactgaaacc agacgatctg aaaggcatga aagtccgtgt ccagccaagc cctggcgcag
     8821 ttgaaatgat caaagtcatg ggcggtaacc cgacgccact ggattacggc gagttgtata
     8881 cagccttaca gcagggtgtg gtcgatatgg cagaaaacag cgtgatggcg ctgaccacca
     8941 tgcgtcacgg tgaagtggca aaatccttca gccttgacga acacactatg gttcccgatg
     9001 tggttctgat gagcaatgct gcgtttgata aacttagccc ggaaaatcag gcagttatat
     9061 taaaagcagc taaagaatca atgagctata tgaaagactt gtggagcgag gaagagaaac
     9121 aagaatttgc gaaactggat aaaatgggcg tgaaagtcta ccaggtagat aaagctccgt
     9181 ttatcgagaa agtacagcca atgtacgcaa acttcgctaa ggacaaccca gcccttgccc
     9241 caatgctggc tgatattcag gcagctaagt aattatcttc gtagggctgc ttattgtggc
     9301 ccttatcgat atctgcaagt gctcgctaca aaagcaaaac gccgaccaat ggtcggcgtt
     9361 tttacgtctc gttgaataac gaattattca gctactacgt ttacgatcac tttcgcgaat
     9421 acttcgctgt gaacctggaa gctcacttcg tgttcgccag tggtacgcag aacgccgttc
     9481 ggcagacgaa cttcgctctt agccacttca acgccagctg cagttacagc gtcagcgatg
     9541 tcgcgagtac cgatggaacc gaacagttta ccttcgtcgc cagctttaga cgcgatggta
     9601 acagtttcca gtgcattgat tttctcagcg cgagcattag cagctgccag aacttcagcc
     9661 agtttagctt ccagttcagc gcgacgtgct tcgaagaatt caatgttttt cttggtagct
     9721 ggaacagctt taccctgcgg taccaggaag ttacgagcat agcccgcttt aacgtttacc
     9781 tgatcaccca ggctacccag gtttgctact ttatcaagca gaataacttg cattacctta
     9841 tcctctcaaa gtcgtattaa tggaccgtga ccgattactg atggcgatca gtgtacggca
     9901 gcagggacag gtagcgagcg cgtttgatag cgcgagccag ctgacgctgg tattttgcac
     9961 gggtaccggt gatacggctt gggacaatct taccgctttc ggtgatgtag tttttcagcg
    10021 tagcgatatc tttatagtcg atctcttgaa cgccttccgc ggtgaaacgg cagaacttgc
    10081 gacgacggaa ataacgtgcc atatggctag tctccagaat ctatcaattc aatctgctcg
    10141 gcatgcaaaa ccattttgct cagtccgttc tttgccttgt ggcatgaaat gaacccctga
    10201 acggttatgc gactgccgac cgttatactg tgagtaatgg cctggttttc gtgtccgcta
    10261 acaataacgg gcatttgaca ccatgcctgc cggtgaaagc cggcttcctc ctgcacagaa
    10321 cgatgctcaa gcacgaactg gcagtgagga attcctgatg gactgacctt tcgaagggga
    10381 gtcctgcaca cggtgccgga caacaccaga cggttggtca tcagaaatta ctcttcagaa
    10441 tccccagctt cagcatcatc agcggtttcg tttgcgaaat catcgcgacg ctcacggcgc
    10501 tcgtctttcg ctttaaccat cggagatgct tcggtaacag cgtgcttggt acgcataacc
    10561 atgctgcgga taacggcatc gttgaagcgg aaggtagttt ccagctcatc gatcacttcc
    10621 tgcggagctt caacgttcat cagaacgtag tgtgctttgt gcagtttgtt gatcgggtaa
    10681 gccagctgac ggcggcccca gtcttccaga cggtggatct tgccttctgc accagtgatg
    10741 gcagcagtgt agcgctcgat catgcccgga acctgttcgc tctggtcagg atggaccata
    10801 aaaacgattt cgtaatgacg catcgaattg ctccttacgg attattcagc ctcctgtctg
    10861 ggtcagccga atcccgggga ggcaaggaac gtgttaaagg tcggctgaaa aatgacgcgt
    10921 tatagtactt gtatgccccg ctaaactcaa gggcagagcc gaaataaatc tcccctttcg
    10981 caattaacct ctaaaccgct gatttggagt tcatcacact tcatcatggt gtttttttga
    11041 acaactgcat cagaaatggt caccatcaac ttctttatca ttgattaaaa ttaaatcaga
    11101 agatcatcac agaggagagc acacaaagtc agccgtcgag gaggtatcta tgttcagtcg
    11161 agttttagcc cttctggctg tgcttttgct aagtgcaaat acatgggcag ccattgaaat
    11221 caataaccac caggccagaa atatggacga tatgcaaagc ttaggcgtga tttatatcaa
    11281 tcataatttc gccactgaaa gtgaagcacg tcaggcatta aatgaagaaa cagatgcgca
    11341 gggcgcaacg tactaccacg taattctgat gcgggaaccg gggagtaacg gcaatatgca
    11401 cgccagcgcg gatatttatc gctagcacca ggtataacca acgaaacatt gccatagttt
    11461 gctttgcccc cttcgcaggg ggcttttttt gaaaaacggc tgcggaatta gaccagttat
    11521 ctcccgaaga aggaaatttc cgcagcgcgt gttttacttc tgcccgtaat aagcgttagg
    11581 accatgctta cgcatgaagt gtttattcat caggaagctg tcgatgtgat tgagttgtgg
    11641 attaatactg cgcgcaatcc acgccatttt cgccacttct tccatcacta ccgcgttatg
    11701 caccgcgtcg tgggcatctt tcccccaggc gaacggtccg tgctgataca ccacaattcc
    11761 cggcgtatgc agcggctcgg cgttgcccag cgtttcgata atcactttgc cagtgttcag
    11821 ttcatactca ccctgcacct cttcttcgct taatccacgc gtacacggga tgtcgccaaa
    11881 gaagtagtcg gcatgcgtag tgcctaacgc cgggatcgcc agccccgcct gcgcccatgc
    11941 ggtagcatga gtggagtggg tatggacaat gccgccaagc gacggataac gacggtagag
    12001 ttcaagatgc gtcgcggtgt cggaagacgg acgatattcc ccttccacca ccttaccgct
    12061 catatcgacc accaccatat cgtctgcttt catggtttcg taggcgacgc cgctgggctt
    12121 gatcaccacc agcccgcgtt cgcggtcgat agcgctgacg ttgccccagg taaaggtcac
    12181 cagcccgtag cgcggcagat ccatgttggc ttcaaatacc tgctgtttta gcttttgcat
    12241 tatgccgcct ccaccatgcc cgctttcgcc atgcgcgctt tcacccaatc acgcgctttc
    12301 gccacttctg ccgccgggtc ttccgccgtt tcgctccaca tctcaatcag gtacggcccg
    12361 caatagccac tctgtttgag cgtttcgaaa caacgttcga aatccactac accttcgcca
    12421 aacggcacgt ttttgaagac gccaggtttg gtgtctttca catgtaccgc gacgatatgc
    12481 ccgattccgg cctgcaactc catctgcaca tcgttgtccc acgccgacag gttgccgata
    12541 tccgggtaga gctggaacca cgggttgttg agatagtgcg cgtaacccag cgccttgctg
    12601 atggagttca tcaacggata atccatgatc tccatcgcca gcgtcacctg cgcgcggctc
    12661 gccatctcaa cgctctcttt caggccgtca cggaaacgac gacgcgtttc gttattggct
    12721 tcctgatagt aaacgtcata gcccgccagc tggatcacgc gaatgccgac gtcctgggcg
    12781 aactggatag ctttacgcat aatctccagc ccctgcgccc gcaccgcgtc atcttcactg
    12841 cccagcggga aacgacgatg agcagaaagg cacatggacg gcacgcgcac gccggtttca
    12901 acaatcgcat tcaccagcgc cagacgctgc tcgcggctcc agtcgaggcg cgacaggcgc
    12961 tcgtccgttt catctaccga catctcgaca aaatcgaagc ccaacgtttt tgccagttgc
    13021 aggcgttcca gccagcactc cccggcgggg agcgcttttt catagatgcc aagcgggatt
    13081 tgtttggaca acatatccgc tccttagccc cacagttcgg cgatggaacg tttgaactga
    13141 cgcgcggctt caaccggaga agcggcatca cggatgctgc gaccggcgat aaacacatga
    13201 attggaatgc ctttgaacag cggcagatct tccagcgcca ggccaccggt aacggtgact
    13261 ttgaagccca tatcggaaag acgtttgatc gcagtgatat cggcttcacc ccacgccacg
    13321 ccagcggcct gcgcgtcacg gctgcggtga taaaccacct gctgaatacc tgcgtcgcgc
    13381 cactgttgcg cctgttccca ggtccagtaa ccggtcagtt cgatctgcac gtcgccgtta
    13441 aactcttttg ctacgtccag cgcacctttg gcggtgttga tatccgcaca acaaatcacg
    13501 gtcacccagt cagcgttggc ttcgaagcac atacgagaaa gaattttgcc cgcatcggca
    13561 attttggcgt ctgccagcac gattttgtgc gggtagagcg ctttcagatc gcgaaccgca
    13621 cgcacgcctt cgcccacgca cagaatggta cccacttcga taatgtcgac ttcttcggca
    13681 atcaggcgag tggtttcgta ggcgctatcc atagtctggt tgtccagcgc gacttgcaac
    13741 atcggtaatg acatcttcaa ttccttctta agctgccgcg ttggtgcggt caatgagatc
    13801 cagtacttcc tgctcggtac ggcaggcacg taaacggtcg aaattttcct catcttcaaa
    13861 caggttgacg atctgcatga tgcccacttc ctggtgcgtg ttggcatcga ccgccgccat
    13921 ggtgatgagg atatccaccg ggtcgttatc ttcgtggttg aactccagcg gctttttcag
    13981 cgtcaccagc gcaaaaccgg ttttcttaac accctcttcc gggcgaccgt gcggcattgc
    14041 caggccaggt gcgagcacaa agtacggacc gtgctgttca accgcatcca gaatcgcctg
    14101 gtagtaacgc ggctcgacaa catccgccgc caccagcagg tcaacgccga tttttactgc
    14161 gtcctgccat gtctcagctt cagcctgcag gcggatggat ttattttccg ccagcgaatc
    14221 acgtaatttc atggcgcgtc cttacttcac atcctgcggg aaatgctctt tgatcacttc
    14281 cagcagtttc gggccaaaat cggcaggaga gagcatgttg cgcacgccaa ccacgtattt
    14341 gttgccggtc acggagatct cgcccgcgat gtgcgtggaa gcgatgataa tatccgcgtt
    14401 gctcagctca cttttgtact cgccaaccgc gcagctgttt accgtgtggt caatctttga
    14461 ttgggttaaa aactggtcca ctttcatttt catgatcatg gaactacctt gtccgttgcc
    14521 acacacagcc agaatacgta cggtcataat caaaactcct tattaagcag actgttctgc
    14581 cagttgtttt tctgcatctt cttcagcgcg cagtgcgcgg ccagcgaaga acatataagc
    14641 cagtgcaatt acaatgatga cggccataaa ggcgataccg atggagaaga agccctgcat
    14701 catcggcggt gccagaatcg accagtccgc catgcccatc caggcgctca taccggtgag
    14761 tttcaccgcc cagacgcagc caaagatttc aatcatcccc atcaccagac atatcttcag
    14821 cgccgcacgc cagccgccga agtggttagc gaacacgccg atggtggcgt tggagaagaa
    14881 catcgggata aagccgggaa taatcaggat cgaggaaccg caggcgacca ggatgccaac
    14941 cgcaatcagc tgaccgatgg tgccccacat aaagccccaa accacggcgt tcggcgcgaa
    15001 gctatagata gccgcacagt caatcgccag aaccgcacca gggatcaggc gctgggagat
    15061 accgttaaat gcttcagaga gttccgccac aaacatgcgc acgccctgag tgatgatgaa
    15121 gatcgccacc gcgaaggaga aaccagtttg caggatgtac accgtccagt tcactttgcc
    15181 tgccatcgcc tgcacggtgt cgataccgaa ggagagcaga atggcaccaa agaagatggt
    15241 catcacaatc gccgtggaga cgatgttgtc gtggaaaata ttcagccagc ctggcagttt
    15301 gaggtcttca acgctttcct cttttttgcc gaggaacggc gcaactttat aggcaatcca
    15361 tgatgcaaac tgttgctggt gaccgatgga gaaaccgcag ccgtccgtca cttcctgagt
    15421 cggcttgtac attatgttgg aggtaatgcc ccagtagagc gaaaccagaa tcgccgtgca
    15481 gataatggtc gtccacatgg agtagccgaa aataaacaac gtaacggcaa tcagccccgc
    15541 ctgctggaac atgatatgac cagtcagcat gatggtacga ataccggtaa tgcgacgcag
    15601 cagcacgtaa cagatgttca acgccagcgc taacaacacc gcgtaaccta cccagttata
    15661 ggcatcgccc atgcggtcga tggttgccat catcgacgcg taagtatcgg aaattgcgcc
    15721 gttaatgccg tagacttcgg acattttcgc caccaccggt ttgaaggtgc tggtgaggat
    15781 gccggaccct gcctgcaaca acatgaaacc aattatggtt ttaatcgtgc ctttaataat
    15841 aacgctgaca cttttgcgca gtaggatgta gcccagacag gtcacaatac ccagcaacaa
    15901 cggggcattg gtcatgacct ggttaaaaaa cacggtaaag atgttgtaga ggatctccat
    15961 aacgatctcc tgaagaagag gtaaccgggt attccacaca cccggaatgt tgtgcactca
    16021 ctctaatttt caaaagtaat cacaacaaga ttatttatga ttaaatgtga cgcgacccgc
    16081 aaataattcc acatggatag aatgggattc acacaacgaa acaactattc atttaaattc
    16141 atttagttaa ttgaattaca cattccatta aatctttcca cactcaaaat caacaaccac
    16201 ttccctggca aaaagtcaaa aaatggtcat tgccttcctg aaaaaatagt gtcaggatta
    16261 atcaaaacca atcacatatt gattcgaatt gaactatcat gaaggtaaat ggcgatgagt
    16321 aaagtgaaaa gtatcacccg agaatcctgg atcctgagca ctttcccgga gtggggtagc
    16381 tggctgaatg aagaaattga acaagagcag gtcgcctctg gcacatttgc gatgtggtgg
    16441 ctaggctgca ccgggatctg gttgaaatca gaaggtggca ccaacgtttg cgttgatttc
    16501 tggtgcggca ctggcaaaca aagtcacggt aacccgttaa tgaaacaggg tcaccagatg
    16561 cagcgcatgg ctggcgtgaa aaaactgcag ccaaacctac gcaccacccc gtttgttctt
    16621 gatccgtttg cgattcgcca gatcgacgcg gtactggcga ctcacgatca caacgatcat
    16681 atcgacgtta acgtcgctgc tgccgtgatg cagaactgtg ctgatgacgt accattcatt
    16741 ggaccgaaaa cctgcgttga tttgtggatt ggctggggcg taccgaaaga gcgttgcatc
    16801 gtggtcaaac cgggcgatgt tgtaaaagtg aaagacattg aaattcatgc gcttgacgct
    16861 ttcgaccgta ctgcactgat caccctgcct gccgatcaaa aagcggctgg cgtactgccg
    16921 gacggcatgg acgatcgcgc ggtgaactat ctgttcaaaa cgcctggcgg ctccctgtat
    16981 cacagcggcg actcccacta ctctaactat tatgcgaagc acggtaacga acatcagatc
    17041 gatgtggcgt taggatcgta tggtgagaac ccgcgcggta ttaccgacaa aatgaccagc
    17101 gccgatatgc tgcgtatggg tgaagccctg aatgcgaaag tggttatccc gttccaccac
    17161 gacatctggt caaacttcca ggccgatccg caagagatcc gcgtgctgtg ggagatgaaa
    17221 aaagatcgtc tgaagtatgg cttcaagccg tttatctggc aggttggcgg caaatttacc
    17281 tggccgctgg ataaagacaa cttcgagtac cactatccgc gcggtttcga tgattgcttc
    17341 actattgaac cggatctgcc gttcaagtca ttcctgtaat ctgtcgttta taccggatag
    17401 cgtggagaag ttcgcgctat ccggcgtagt atttcctggc tatttcaaat atcatctaaa
    17461 aaaatcaaat tttatcggaa tagctcatga ctgaagcaca aagacatcaa atcctcctgg
    17521 aaatgctcgc acaattgggc tttgtgaccg ttgagaaagt cgttgagcgt ctgggaattt
    17581 cgcctgccac tgcgcgacgc gatatcaata aacttgacga aagcggcaaa ctgaaaaagg
    17641 tgcgcaatgg cgcagaagct attacccagc agcgcccgcg ctggacgccg atgaatctgc
    17701 atcaggcgca gaatcacgat gaaaaagtac gtatcgctaa agcagcctcg cagctggtta
    17761 atccgggcga aagcgtagtc atcaactgcg gctccaccgc gtttctgctt gggcgggaaa
    17821 tgtgtggcaa gccagtgcaa atcatcacta attatctacc gctggcaaat tacctgatcg
    17881 atcaagaaca tgacagcgtg atcattatgg gtggacagta caacaaaagt cagtccatca
    17941 ctttaagccc gcagggcagc gaaaacagtc tctatgccgg gcactggatg tttaccagcg
    18001 gaaaagggct gaccgcagaa gggttgtata aaaccgatat gctgacagca atggcagagc
    18061 agaagatgct gagcgtggta gggaaactgg tggtactggt tgatagcagt aagattggcg
    18121 aacgcgcggg aatgcttttt agccgtgccg atcaaatcga tatgctgatc accggcaaaa
    18181 atgctaaccc ggaaatcctg caacaactgg aagcgcaagg ggtcagcatt ctgcgtgttt
    18241 aaagatgctg gcggaaaaat gtcacggcag catctaacgc ctcaggcgta atgcggtgac
    18301 gcacgcctgg ctgccatgaa caggttagca gtttatcccg tcccgtctcg cttaaggcct
    18361 gctgcaaacg tagtgattcg tcggcaggca caacgtcatc gtcgaggcca tgccacagca
    18421 gtagaggtct gtccccaagt tgttccaggt ggtttgtcgc ttcccactct gccagcggcg
    18481 cgataatgtt attgaattca ttctgctgtg ctgccgtttc aggtatcagc ggtggaaaca
    18541 gtgaacgggc gagcgatgta aaatagcccg atcccatcat gctggcggta catttcaccg
    18601 tggggtggcg agcggtaatt cccagtgccg tcatcgcgcc catcgacgcg ccaccgactg
    18661 ccagacggtc atcaagcagc cagttttctt cggctattgc cgcacgtaaa gtagtgaatt
    18721 cctgcatact ttgtagcaag atttgccaga attgatttaa ccgccgcgct tcgtcgctac
    18781 taaaacggct accgtgatcg ggcgcatccg gcatgatcac ccgcaaacca gcctgcgcca
    18841 gcgcaacggc aaaatagcta tacaccaggc tggatgaagt aaagccgtga taaaaaatta
    18901 cgcacggtaa cggggtatct ttttgcccga caggataagc atgaagaacg ggaatatctg
    18961 ccagctcgcg tgattctatt tcaatcatct gtctctcctt tttgtctggt atgacatgaa
    19021 gaaatcctta gcacaagttt ctgctggcgg tattagttca taatgttgag atgtgggtta
    19081 cgctttcgtc acgttttcat tcgaaaatcg cgtaccaggt aacaattcgg gaacattccc
    19141 ccaaagttaa attaataagg cactacacta tggttagcag gaaacgtaat agcgttattt
    19201 accggtttgc cagtttatta ttggtgttga tgttaagtgc ctgtagcgca ctgcaaggta
    19261 cgccacagcc agcaccgcca gtaacgaacc atccgcaaga gattcgccgc gaccagacgc
    19321 aagggttaca gcgaataggt agcgtaagca cgatggttcg gggttctccg gatgacgcat
    19381 tagcagaaat cagagcaaaa gctgtcgctg caaaagctga ttattacgtt gtcgtcatgg
    19441 tggacgaaac catcgtgaca ggacagtggt attcacaagc cattttgtac cgtaaataaa
    19501 tcagacaact tacatagtca gctttacatt gctttgcgtc cctatgcgtt tacctgtgca
    19561 caataaatta ccggctggaa gcctgggcgt ttattaccgc gatggaatgc cctgcaacat
    19621 gtggggaaac gaaaaatgga gctgacgatg aaacaattac ttgcctcacc ctcgctgcaa
    19681 ttagtgactt atcctgcgag cgccacggca cagtctgcag aattcgccag tgctgactgc
    19741 gtgacgggcc tgaatgaaat aggtcagatc tcagttagca atatttcagg ggatccgcag
    19801 gacgtggaac gtatcgtggc gttaaaggcc gatgaacaag gtgcgtcatg gtaccgcatt
    19861 attaccatgt acgaagacca gcagcctgat aactggcgcg tacaggcgat actgtatgca
    19921 tgagtgtcat ctacagcaaa tgaacaatgc cggatgcggc gcgagcgcct tgtccggcct
    19981 acaattttca catatttcat tgagttaatc gaaaccagcg tcgcatcagt cgtatacgct
    20041 tacacacaca ctccccccgt cgcccgcagc aataaatcat tctggatctg ctctgacaac
    20101 cgtacgccgc cgcgcgtatc taacatcacc tgacaccacg cctgtgccat tggcggagag
    20161 gcatatttca acatttgcgc accgcagccc agcaggaata gctgatgagt aatctctcgc
    20221 cccaattctt cagctggctt gcgcagctgc tgctgtaaac gacgaaccgc gcgatcaaaa
    20281 tagcgatcct gccctttcac ttccacaaac gcttccgaca ataagtcgta tacgcccgct
    20341 tgcttattga gaacgcgcag cacatccagg cacataatat tgccggaacc ttcccaaata
    20401 ctgtttaccg gcatctcccg gtaaagccgc ggcagctcgc tctcctcgca ataaccaatg
    20461 ccgcccagca cctccatcgc ttcggccaca aacggcatac ctcttttgca gatcacaaat
    20521 tttgccgcag gcgtaaataa acgcgcccac agggcttctt tggcatcggc acgccggtcc
    20581 cacgctcgcg caagacgaaa cagcaacgcc gtttgccctt caagctgaag tgccatgcga
    20641 cttaagacat gacgcataag gggctgttgg atcaatggat taccaaaaac atggcgttga
    20701 tgtgcatgat aaatcgccag cgaaaatgca cggcgcatca tggcatggct acccagggcg
    20761 caatcaaaac gcgtcatccc acccattttc aggatcagac gaattccttc cccttcctgc
    20821 cccaacaacc agccaatggc atcctgaaac tccacttcgc agctggcgtt agagcgatta
    20881 cccagcttat ctttcagccg ctcgaggcga atcgcgttgc gttgcccgtc aggcaaaaag
    20941 cgcggcacaa aaaagcagga caaaccgccc gtagtctgcg ccagcaccag atgcgcatcg
    21001 ctttgcggca ccgagaaaaa ccatttatgc cccaccagcc gataagagcc atcttccagg
    21061 cgctctgcgc gggtggtgtt gctcatgaca tcggaaccgc cctgcttttc cgtcattccc
    21121 atgccaatca acaaaccgcg tttttgccca cctggcaata agtgagaatc gtagcgatcg
    21181 ctcagcagcg gcgtggtcca gtcctgaaac ggcgcgggta acatctgtaa cagcaatggc
    21241 gtggcggcaa aggtcatggt tatcggacat aacgaccctg cctcaacctg tgcgtgcaac
    21301 ataaaacgcg ccgcgcgcgc cacaaatgcg ccggagcgag cgtcttcttc ccaggcaaga
    21361 ttgtgcaccc gattggtaca tagcgcctgc atcagcaggt gccaggcggg gtgaaaacgc
    21421 acatcgtcca gacggcgtcc ttgcgcatcg tagcgcaata attcaggcgg attcacattc
    21481 gccagccgcc ccagttcaag ggattcagcc gttcctaact gctgaccaat actggctaga
    21541 aaatcgctat cccagccagc accttcacgc gttaccgctt cgcagagcgc gccatcagac
    21601 aggtataagt tgctgttatt taatggtata ggttgattaa aaacggtgtg agtttgccag
    21661 tgcactgtgt ctccctccat caatggcagt caccattagt atggtcactg ccatggattc
    21721 atgacagaaa gagattgcta aaacattctt atccagcaat gtaatgcgga acgaaacgtg
    21781 aggtgtcttt ggtgatcagt gtgttatctt cacggatccc cattccgcac gcttcatcat
    21841 cgacaatcca gctaccgatg agtgtgtagc tatcgccaaa tcgcggcaga ggttgaaacg
    21901 cctggtagat catcggttca tcggcgtaat caccatcagc gtggtcaacg acgttattct
    21961 gaccgtcgaa aatggtgacg tttccgcctt cgcgcgagta gatcggttta cgcacatagc
    22021 tttcgccagc ggcaatctgc gatttttcac cctcaaacca ggatgcaaga aggttaggat
    22081 gaccagggaa gaagcgccaa agcagcggca ttagcccttt attactcaag atacttttcc
    22141 ataacggctc cacccattgc tcgcgacgct tgcacagcag cggaccgtta tcgtcacgca
    22201 tcatccactc cagcggatac agcttaaatg cacgctggat gacattatca tcaagatcgg
    22261 tcagtacacc gccgacgccc aaaccgagat cttcaatgta gataaaccgc gactcctgcc
    22321 ctgcctgctg ggcgcagtcc tgcaagtaca gcacggtagt cctgtcttca tcggtgtcct
    22381 gacagcagca gaaataaaac ggctcccggc tataaagctc gctaaagcgt gaaatcaggc
    22441 gttcctgaat ggcattgtac tgatcggcat cacgcggaat aatgccgctg cgccgggcat
    22501 cctccagcca caaccactgg aaataagccg actcatacaa cgaggtcggc gtatcggcgt
    22561 tgtactccag cagcttcacc ggcgcattgc cacaccaggc aaaatccatc cggccataca
    22621 gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc ccagtacaac ggcgggatcg
    22681 ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc caccacctca aggcacatct
    22741 gatgcagttc cgcagtcggt ttttcgatct gctcttcaat ctggcgcaga gtaaaacggt
    22801 aagcccgact ctcgtcccaa tagatttcat tgtcgatgat atgaaagtcg aaaccgttgt
    22861 tagcggcgat ctggtccaga tcccgtcgca caggaacgtt gtgtctcagc atgattagcc
    22921 tccccaatgc ccacgggcgc tggaagaacg accatagccg ccgcgcgaaa cggttgacgc
    22981 ttttttggtg gtaaagccgc ctgaagagta agactctttt ttgccagaac cggagcgcca
    23041 ggagtaatca ccggaagtgt tgcgccagac ggggcgggaa gcaaatgagg atccgccgct
    23101 gttatagacg aacggctcat cgcgatcttt acgaataact cggcttaaca aaaatccaga
    23161 aacgaccggg atccaactct gttcaacatt gtcgtagtag caattttcgt acttagactg
    23221 acaattctgc tgagtcatat tcttcggaac atcggcataa aatgccgctt tggcgttgtt
    23281 ccagccacgc gcgcaaatgt cggcattatt accgtcatca atacaatcct gcactgtcga
    23341 ataaaacgtt ccgtcgccgt cgttatcgac atcgccactt tcactccatc ctttgactat
    23401 taaaaaaacg gcaccgccca ttagcaccaa cgttaagtat ttttgtgcgt agcgattgcg
    23461 gcgcggctca aaaggattat gcggtcttcc aatgcgactg atcgctccgt gaccaatttt
    23521 actgttattt cttgatttgc gttttctggc catgatcgtc cattaccatg tcatacaagc
    23581 tgcgttaaaa ataccaccag ccagtgcggc ggttcccata aacattcctg ctgcggtatt
    23641 gtgattaata attttttcgc ttaatgcggg catataaagt ctgacaccag caaaaacgag
    23701 taactgaatc accagtgcaa tcccgcccca ggcgaaatag tctggaatac tcaccgcatt
    23761 gattgccgca ctggataagg ggatcacgta acccaacaat gtaccgctga atgccagtga
    23821 cgccgcggta ttattgtttt tgattaattg ccattcgttg tgcggtgtaa ttttggagta
    23881 gataaacaga aaaataatca ccatagccac gccaataaaa aaaataggca ctaaaggcaa
    23941 gaagtgaatc cagtatatgc attgtaaacg tccttgtaat taaccaataa tatgaagtga
    24001 tgtcagcggg atatcgacac ccaacgcgcg cgaaaataac cactctggct cgccgagatc
    24061 gttaaaagat tcctcaccat ttaacagcaa atattcgtaa gtatcttcgg ttacttggcg
    24121 ttggtagccc atggtaaaat tatggacctc ccatttggca tggttttgat tttctacttt
    24181 ttccagcatg tagacgggtt cgatatttcc cggttcttcg ctgttaaaaa atcgctgcca
    24241 gcgtttttcc tgccagttta agatcattgc ccccatcact ttggcgttga tcgcctcgcg
    24301 ccagtgactc tctttactga taccgtagct ctcttcatag acaaagagct tgatatcatc
    24361 aatgtcatca atatcttcgc cgccggtggt attgatttgc agaaattcat cgcctgaagt
    24421 gtagtagcgg aaaatctgac taccgccgcc cagatcgatg tggctgacag cggctaccgt
    24481 aaattcctca cccggcagcg caatcagcaa ttcatcttcc agcaaacgaa acgctaacgt
    24541 gtcgagcgta aagccactat tgagatgaag tcccagcgga ccgcgagcga tagcaggctt
    24601 attatccttg ccaaacagac gctggaaaaa accagacata ttttatcctt aaaatagttg
    24661 ccagcctttt agcgaggctg gcaaaaaaat tactcgccct gttggcgttg cagtcttgct
    24721 aatacatcct gggcgctact tttattgcta ccgccaattc cggcttccgc cagcttttca
    24781 tcaagatcgc gaccgtctgc gactttttcc aactgtgcag cagcatccag gcgagcctga
    24841 cgttcggcct gacgcgtttg caggcgtttt aaggattctg ccgctgtcga aacgctggaa
    24901 gatgcgccaa cggtagaggt tgttacagcc tgttgtgcac gctgcatggc ttcagtggct
    24961 ttaacgactt ccatttgctg ctcaaactga gcgatacgct gcgctgtcgc tgtaactgct
    25021 ttttccacgc catcacggga gacttccaga ttcgacaaca cttgctcttc agcggtaatg
    25081 agattctcaa ggcgcgcgat ttcttcagca acttcgttaa tcaacgacgg attaacgttc
    25141 ttgcttaacg cttccagcgc acgggcttcc agactggctt tgcgctcacg taaatctttc
    25201 agcttatcgt gacttaattt cacccgcgcc agcagatcaa cgcgagattt tccggcttta
    25261 tcgagttcag ctttagcgtc acgaatatgc tgttccagca tgcgcacgcc ctgggtttct
    25321 tcaatggatt cttccgcctg ggagataaac gatttcccca gcgtaaataa actttttaaa
    25381 attcccataa atacccgtcc ttgtaattag tgtgaatatt cttctgtaat ttcagccaga
    25441 tccaatgcgt tatctacctg cgaggttatc tcaagcagga tatcttcaag agaagattta
    25501 agcgataacg ccccgaaaac aatgtaatac tcttcctgtt gcacgctgga gattcctacc
    25561 gatgacaacg gcatcatctt ctgatttctt aataagaagg tattaaattc atctggattg
    25621 ctgatactac ttaccggaca aataaaggtt tcgataatca tttggcggga agtaaaaaga
    25681 atattgattt gcaaatcgcc ataatcattc attttaataa ttaatgcgct ctcgctattt
    25741 gtcacatcaa tattttgttc aggtacagtt tgcagcgccg tcgccagcgc caacggattc
    25801 catgtcatat attattccat atagatttag tttaaatatt aataaaatga atatttgcag
    25861 tacgtaatta tcttaccagc tatagacaaa aaaaaaccat ccaaatctgg atggcttttc
    25921 ataattcaga cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca
    25981 accgaaacgt tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa
    26041 tgttcacggg tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaggcgt
    26101 ccggtcattt tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag
    26161 atattctctt cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga
    26221 acgctttctg ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga
    26281 tctttcggca caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg
    26341 aggttgtgcg gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg
    26401 atcagatccg gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg
    26461 ccctgatgca cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata
    26521 accacgccct gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct
    26581 tttaaaataa agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg
    26641 tggatgccgt aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca
    26701 ctacgtttat ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc
    26761 cggataaggc gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac
    26821 cctcttcttt taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg
    26881 ctttggtcgc tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt
    26941 ctttcttcgc cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa
    27001 agtttacctt tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt
    27061 tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac
    27121 taaagtcgat tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc
    27181 ccaggcgata agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc
    27241 gatagtagtc attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca
    27301 gacggacgaa gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta
    27361 cctggtcgag catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg
    27421 cacgacgttc cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat
    27481 ggtagccgcc ggtttcagtg gtgttgccct gatgcccctg ctctttcgac agcagatatt
    27541 taatggcgcg gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat
    27601 aggactgcaa tgccagaccg aagtgaccac ggttttctgg atcgtaaatc gcctgtttca
    27661 tcgagcgtag cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca
    27721 gcagttccgc gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct
    27781 ccgccagcac tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga
    27841 acagtgccgg ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca
    27901 tgcactcttc aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac
    27961 ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt
    28021 cttcacgggc tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca
    28081 gcggggcgta ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt
    28141 aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc
    28201 ctttcgacga aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg
    28261 aacacaggcc gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca
    28321 ccgacgtgcc acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc
    28381 tgacgtcggc aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa
    28441 ctgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca
    28501 aatcgacacg gcccgctttt gcttcttccg gcacttcttc tttcagcccg gcaacctgtt
    28561 gctcaacagc ctgcggccag atgtacggaa tttcatgggt acgcagagcg atatcaaccg
    28621 ccatgccggt gcccatatta tcgccaagga cttcgacgat tttacccacc gctttggtgc
    28681 ggcgagtcgg acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga
    28741 tctgatcggg cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc
    28801 cgacgcccgc ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc
    28861 ggacaatacg cgcttcacga cgacctttac ggtcagcgcc cagcggctga gccagcacct
    28921 gatcgccatg aatgcaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc
    28981 cttcaacccg cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt
    29041 cgaggcgttc cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct
    29101 ccatcgcgcg caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt
    29161 ctaccgccag ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa
    29221 attcccgact agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt
    29281 gtgacatctc ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa
    29341 taatttataa agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc
    29401 cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat
    29461 gtggcaaaac tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc
    29521 accaatacgt atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac
    29581 agcagtcacg tagccggcac gactaagttg attgattatt ttgaccatat gattacggga
    29641 gacgccgtag acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc
    29701 catgtagatc agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc
    29761 aaaagggaaa tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc
    29821 gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc
    29881 gtaatggctt aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg
    29941 ccaggcgtac cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc
    30001 cggaccggta gagatgatat cgatcggcac accagtcagc tcttcaatac gcttgatgta
    30061 gttcagcgcc gcctgcggca gaccgctacg atctttcacg ccgaaggtgg attcagacca
    30121 gcccggcatg gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt
    30181 ggtcgtcact tcacgaccat ccggcatacg gtaagccaca cagagtttca cctctttcag
    30241 gccatccaga acgtccagtt tagtcaggca gaagccagac agggagttca gctgtaccgc
    30301 acgacgaacg gcaacggtgt ccagccagcc ggtacgacga cgacgccccg tagttgcgcc
    30361 gaattcgtta ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa
    30421 cggacctgca cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata
    30481 acgcgggccc aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac
    30541 gtacggataa gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac
    30601 gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt
    30661 caggatgtcg gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc
    30721 tttgtagtag ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc
    30781 gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc
    30841 ataagcaggc ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc
    30901 acgcgcgtta tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag
    30961 cagacgctca cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc
    31021 ggccggagac agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc
    31081 tgatggaata agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt
    31141 gtgaccgccc tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt
    31201 acctttacct tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca
    31261 aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt
    31321 tgcactcaaa aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca
    31381 tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat
    31441 cgggcaaatt ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc
    31501 ctaaaccttc cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt
    31561 atccttatag aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg
    31621 taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat
    31681 cgctatccgg gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac
    31741 gcaggctacg gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt
    31801 tggctgcttc ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca
    31861 gcgttctggt cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt
    31921 gtgaacggtg acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt
    31981 cagacacttc ggtcggcagg ttgatctgct taattcgcac atcgacaacc tcaataccca
    32041 gcgccgccat actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc
    32101 gctctgccgc ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac
    32161 ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg
    32221 tgacgatatc tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac
    32281 gtttcaacag cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc
    32341 tgaaatcgct gatgcgccat ttgatgtaag agtcgacgat caggtctttc ttctctttgg
    32401 tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg
    32461 tttcaatgaa cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat
    32521 cgtcacgcag taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa
    32581 agacagacat gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca
    32641 tcgttattcc ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat
    32701 aatatcgccc tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc
    32761 tggcggcaga cgcagcagat tgctggtacc gttatcgctc ttcgccgcag gggcgttacc
    32821 gcctttcagc atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag
    32881 cactttgcgg gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt
    32941 aatttccggc gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg
    33001 agcttccagg atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg
    33061 accgttcgca cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg
    33121 ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg
    33181 agcagcctgg aagttgacgt ccagcagcgt gatacccatg tcatacggac ggatagtctc
    33241 ttccagttcg cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc
    33301 catggtgtat ttaccgataa ccccgcgcag ggcgctgtcg gtagcctgac gcaggctgtc
    33361 atccgggctg gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt
    33421 catctcaacg cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc
    33481 acgcacggct tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag
    33541 acccggctca accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc
    33601 tttaatggta tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt
    33661 aacgacacga ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc
    33721 ggtgccttta ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc
    33781 taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc
    33841 aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc
    33901 catgtttgtg ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat
    33961 atgactccac gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt
    34021 gtttacagag gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt
    34081 cctcctccat ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgcc
    34141 cttcctgcgg cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca
    34201 aagcctgaaa aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg
    34261 gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt
    34321 tcatcaccag cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg
    34381 cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc agtaatgtgg
    34441 cttgccgcgt ctcttgtaac gtggctttaa atgccgccac cagatcgtgc ggcaggtggc
    34501 gaataaaccc tacggtatct gcaagtacgg tttcaccgac atctgcaacg tcaatacgcc
    34561 gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga
    34621 tgcgattgaa aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa
    34681 cgtcggcttt gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttccaggc
    34741 gtgactgtat ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt
    34801 cacccggacc acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca
    34861 ccaggcgcgt agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg
    34921 tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact
    34981 cgcacaaacg ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga
    35041 ccgaagcacc cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa
    35101 aatactttgg gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg
    35161 cggaagagac cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat
    35221 agatgtgtac cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc
    35281 tctaaataga tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg
    35341 aaaccttatt cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca
    35401 tgatggtagt tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga
    35461 gacgggacaa cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac
    35521 aggatcacga actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa
    35581 taaatagaaa ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat
    35641 tgccccttag ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt
    35701 ctgaaaaaat tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac
    35761 aacctgtaat acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc
    35821 ttcccaacca cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc
    35881 tcgataaacc atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata
    35941 acccacgcaa cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg
    36001 gacttctgct tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg
    36061 gagcagttca cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc
    36121 tcctgacgtt tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc
    36181 ccgggaaagc ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc
    36241 ctgaagttga cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc
    36301 ccgcacttcc gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata
    36361 caacatcgta ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc
    36421 cgccagcgca tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc
    36481 cagcaatcgg tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc
    36541 catccctttg taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg
    36601 cagctcaatg gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt
    36661 aggcaggctc gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat
    36721 caacagattg taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca
    36781 cgtccgccag cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat
    36841 ttcgtgcaat ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc
    36901 ctatcagttc aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc
    36961 tgatggtcac atgctgtgca tctgactgga aatcaatacc caattccgct agggcagact
    37021 gcgctttttc taatgccgat ttttcttcgg cagaaacttt tagccgcaac ggaatcagca
    37081 gcggctgggc gcaaacgggc gcttcacccg gcgtcaattg tgcctgacgt agccaacgtt
    37141 ctgccactgg caaggataaa agtgaaatgt tgccatcgcg ctccagcaac gcacagtcgg
    37201 aatggacgat agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct
    37261 gcggttccgg cgcttttggt ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca
    37321 cttcaccttg ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac
    37381 tgcctgatgc tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg
    37441 caaagtgatt gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg
    37501 gttcatcgtc cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct
    37561 gatagataaa atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt
    37621 gcacgttgac gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct
    37681 gatcggcccc cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc
    37741 gcatcatgcg accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat
    37801 ttggatcggc cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg
    37861 cttgttcaag aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt
    37921 ccggcactgc gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt
    37981 cgaaacgcgc cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct
    38041 cggtgcgcag gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg
    38101 tcgtccccac aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat
    38161 aggcctgcca ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg
    38221 aactgatact cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt
    38281 ccagagaggc gattttactg gtggcatgac gcgccagcgc cagcgccagc tcgtcttttt
    38341 tgataccgca gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa
    38401 tatcgatacg tgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg
    38461 caggtcgctc gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct
    38521 gaattggcat caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg
    38581 ccgtttgtgc cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc
    38641 gcaggccttt gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac
    38701 gttcttcgct gttgttgctg ataaaaccgg tttcgaccag tactgatggg atatccggcg
    38761 aacgcagaac gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt
    38821 gcaactgact gatcatactg gtcgctacat cataccctac ccgttgggaa tgaccgaact
    38881 gtaaatccag caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcac
    38941 ccgccccacc cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc
    39001 tgttggcgcg acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg
    39061 cggcatcagc gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcactgc
    39121 gccccatcac cgagataaag taatccccgt cacgggttaa aacgccctta aacatcggat
    39181 cgtcattgag caaagtacgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg
    39241 taccaccggg gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga
    39301 taattttatc gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc
    39361 tgataacacc cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga
    39421 cgcgcggtgc gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggcggaggag
    39481 gaggtggcgg cgcatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc
    39541 gcttcaccgc ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg
    39601 catctttagg cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg
    39661 gcagtccctg aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat
    39721 ggctaaacgc ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac
    39781 cgttagaaac ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca
    39841 gcgtcgctac caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac
    39901 cggctaaacg cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac
    39961 ggccttgtgc ctgataatca atgtgtattt cgacatccgg gtcaggaaga acaccagtac
    40021 cttgttgtgg ccactccacc aggcagatgg catcgttggc aaaataatcg cgaatcccca
    40081 taaactccag ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat
    40141 tgtcgagcgt atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac
    40201 ccagagcttg taaaaagccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca
    40261 gatagattac ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg
    40321 cctgctcatc agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg
    40381 ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa
    40441 cgcgccgcca gtacgtcagc tgcagcaccg tgcgcgacac agcccgcaca ggcggcatca
    40501 tacggcgaca gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg
    40561 ccgccgctcg ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg
    40621 gcgaccacgg ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt
    40681 ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg
    40741 gcctcgccag gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc
    40801 aggttcaatg catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt
    40861 gcttttttcc cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat
    40921 tccaggcttt cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca
    40981 gtcagcagcg gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca
    41041 cgtagcgccg cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata
    41101 attaccagcc gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt tagccagtga
    41161 gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccaactatcc
    41221 agccccagtg agtcaaaatg cagttgtccg gtaacatccc gcgcttttcc agtgagcaag
    41281 cctggtttca gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta
    41341 gcgccggttt cagccagcag gccggaaggg atatcaaccg ccgcaatcgg cgcaggatgg
    41401 gaattagcgt ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta
    41461 ccgagcagcg catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg
    41521 atctcgcctc ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt
    41581 ttgtcgctct cctgggccag cagcgtgacc tcaatgccga ccgctgtggc cagccgcgcg
    41641 accacgtagc catcgccgcc gttattacca tgaccgcaca gcaccagcca atggcgggcg
    41701 tcaggatacg ccgaacgaca cacctggaat gcggcttcgc cagcgcgaag catcagctca
    41761 tagagtgtaa gccccagcgc atctgccgcc tcacgttctc cgcggcggat atcgtcggcg
    41821 tgccagacgg tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca
    41881 gagcccctcg atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc
    41941 tttcagcagg taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca
    42001 tggctggaca aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc
    42061 gctcgccccc atgagttatt gcccggtacg ctgcgcgtga tcagcgtgcg gatgaattac
    42121 cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc
    42181 cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc
    42241 gagatgattc agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct
    42301 attctcgagc gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt
    42361 atcctcaatc gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg
    42421 ctgcccgtgg atcaaccagt cgaggaagga tgcggtaaat gcgtggcctg tatgacgatt
    42481 tgcccgaccg gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat
    42541 ctcaccatcg aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt
    42601 atttacggtt gcgatgactg ccagcttatc tgcccgtgga atcgctattc acaactcacc
    42661 acagaagagg atttcagccc gcgtaagccg ctacacgcac cggaactcat tgagttattc
    42721 gcctggagcg aagagaagtt tttaaaagtc acggaaggat cggcgattcg tcgtattggt
    42781 cacctgcgtt ggctgcgtaa tatcgccgta gcattaggca atgccccttg ggatgaaacg
    42841 attttgacag cgctggaaag tcgtaaaggt gagcacccac ttctcgatga gcacatagcg
    42901 tgggcgattg cgcagcaaat agagagacga aatgcgtgca tagtcgaagt gcaattgccg
    42961 aaaaaacagc gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc
    43021 cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa gacgaataaa atcgtcaagt
    43081 gatcgactcg acaaaacgga atcttaattt ttaacttatc tatcaatagg ttaaaaaaat
    43141 actattcacc gtgcgaagtt tctttgaaat taagattaca gcctgaggct gtggataact
    43201 ctgtttacaa gagtttgtca gagacaacga aaaccagcaa taacgcatcg catcgctgtg
    43261 gatattttat tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac
    43321 cttggcaagg tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca
    43381 gcactttcaa attttggagc gggaaacgag
//
LOCUS       DAZFYC010000028        43297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFYC010000028 DAZFYC010000000
VERSION     DAZFYC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.028"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKW5_001726"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKW5_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO7511136.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKW5_001727"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKW5_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7511137.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKW5_001728"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKW5_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO7511138.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHKW5_001729"
     CDS             complement(1087..1581)
                     /locus_tag="ACHKW5_001729"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO7511139.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKW5_001730"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKW5_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO7511140.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKW5_001731"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKW5_001731"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO7511141.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKW5_001732"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKW5_001732"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO7511142.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKW5_001733"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKW5_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO7511143.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKW5_001734"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKW5_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO7511144.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKW5_001735"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKW5_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO7511145.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKW5_001736"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKW5_001736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO7511146.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHKW5_001737"
     CDS             complement(9275..9769)
                     /locus_tag="ACHKW5_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO7511147.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKW5_001738"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKW5_001738"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7511148.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKW5_001739"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKW5_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO7511149.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKW5_001740"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKW5_001740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7511150.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHKW5_001741"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHKW5_001741"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO7511151.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKW5_001742"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKW5_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO7511152.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKW5_001743"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKW5_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO7511153.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKW5_001744"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKW5_001744"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO7511154.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAETRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKW5_001745"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKW5_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO7511155.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHKW5_001746"
     CDS             17948..18874
                     /locus_tag="ACHKW5_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO7511156.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGEIGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKW5_001747"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKW5_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO7511157.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHKW5_001748"
     CDS             complement(19225..21444)
                     /locus_tag="ACHKW5_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO7511158.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKW5_001749"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKW5_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO7511159.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKW5_001750"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKW5_001750"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO7511160.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKW5_001751"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKW5_001751"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO7511161.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHKW5_001752"
     CDS             complement(26816..27298)
                     /locus_tag="ACHKW5_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO7511162.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKW5_001753"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKW5_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO7511163.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKW5_001754"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKW5_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO7511164.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKW5_001755"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKW5_001755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO7511165.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..30278)
                     /locus_tag="ACHKW5_001756"
     CDS             complement(29946..30278)
                     /locus_tag="ACHKW5_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO7511166.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            30597..31178
                     /gene="mdaB"
                     /locus_tag="ACHKW5_001757"
     CDS             30597..31178
                     /gene="mdaB"
                     /locus_tag="ACHKW5_001757"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO7511167.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            31209..31523
                     /locus_tag="ACHKW5_001758"
     CDS             31209..31523
                     /locus_tag="ACHKW5_001758"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO7511168.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(31571..33463)
                     /gene="parE"
                     /locus_tag="ACHKW5_001759"
     CDS             complement(31571..33463)
                     /gene="parE"
                     /locus_tag="ACHKW5_001759"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO7511169.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="ACHKW5_001760"
     CDS             complement(33492..34073)
                     /gene="yqiA"
                     /locus_tag="ACHKW5_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO7511170.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="ACHKW5_001761"
     CDS             complement(34073..34900)
                     /gene="cpdA"
                     /locus_tag="ACHKW5_001761"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO7511171.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(34925..35347)
                     /locus_tag="ACHKW5_001762"
     CDS             complement(34925..35347)
                     /locus_tag="ACHKW5_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO7511172.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="ACHKW5_001763"
     CDS             complement(35348..35977)
                     /gene="nudF"
                     /locus_tag="ACHKW5_001763"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO7511173.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARLEAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            36182..37663
                     /gene="tolC"
                     /locus_tag="ACHKW5_001764"
     CDS             36182..37663
                     /gene="tolC"
                     /locus_tag="ACHKW5_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO7511174.1"
                     /translation="MKKLLPILIGLSLYGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            37811..38482
                     /locus_tag="ACHKW5_001765"
     CDS             37811..38482
                     /locus_tag="ACHKW5_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO7511175.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            38488..39648
                     /locus_tag="ACHKW5_001766"
     CDS             38488..39648
                     /locus_tag="ACHKW5_001766"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7511176.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQIEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="ACHKW5_001767"
     CDS             complement(39686..40474)
                     /gene="ygiD"
                     /locus_tag="ACHKW5_001767"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO7511177.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            40617..41390
                     /gene="zupT"
                     /locus_tag="ACHKW5_001768"
     CDS             40617..41390
                     /gene="zupT"
                     /locus_tag="ACHKW5_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO7511178.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(41448..41618)
                     /gene="yqiD"
                     /locus_tag="ACHKW5_001769"
     CDS             complement(41448..41618)
                     /gene="yqiD"
                     /locus_tag="ACHKW5_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO7511179.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="ACHKW5_001770"
     CDS             complement(41879..42532)
                     /gene="ribB"
                     /locus_tag="ACHKW5_001770"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO7511180.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(42636..42784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            42906..43196
                     /gene="ubiK"
                     /locus_tag="ACHKW5_001771"
     CDS             42906..43196
                     /gene="ubiK"
                     /locus_tag="ACHKW5_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO7511181.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        10770 a        11559 c        10815 g        10153 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcaacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcagca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggta
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcgcagc agtgaacggt acgaccaata atacggccca gcgtagagtg cagttgcgcc
     2041 acttccagcg tgttaccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg ccccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc gggctgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat atccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca catatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gagatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgtgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctagccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgaaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gttgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tataagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactaca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc acccgctgat gcaacaacgc cagctacatg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaactccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg cattgtcgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaat cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 cacgcccgta aaacctggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagaacagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga aaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gtggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gtggctgcac ggttacggct ttggcttcct gctttttcgg
    20641 tgccaccggt ttgttatccg cgcacggcaa atcttcacca tacggatgcg ggattgggtc
    20701 gatttttcca ggggtatcaa gacgggtgga atccacgccg ctccagccag gcagcgcctc
    20761 tttcacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    20821 cagacggtgc gccacctcca ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgtgcggt
    20941 acggcgcaga ctagcctcaa taccgccgag gatcaccggt acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg
    21061 cgtgtaggca tcgtcatgac gtaaacggcg atcggcggta taacggttga tcatcgaatc
    21121 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca tgaaatcgtc
    21181 tttgctgctc cagtctggct gggcgatgat cccgacgcga aagccctgtg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaaaagtt gccctatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acattacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagactg
    21901 atatcgcgac tgcgaattgg cgaaccagcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accagaatac tggatggctc aaagaagccg cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcgccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaataggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccaa
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagttcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct ccaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat tatgtataac
    30241 agagcttcaa atattgtttg aattttatgc tttaacatat atcagtacgc tcataaaaat
    30301 ccatttataa ttatcaacat gacacgttga tattttcatc ttttacttat tcaaagccat
    30361 attttcaatc ccaaaaaaaa ccgaaccaaa cgtgtaaatt attccggtgc gagtaagaat
    30421 tatgaggaat ggctatcagt attgtcattt tcagaaaata tttatcctgc atcggtgagt
    30481 cagagcaaga tcagactttt gctaaattcg caaaagactt tgcacatttt gctaatttca
    30541 ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt taaaaaatga
    30601 gcaacatcct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt caactgaacg
    30661 acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat gtccgcatcg
    30721 ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc tgggctgatg
    30781 tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg aaaaaatata
    30841 ttgatgatgt attcaccgaa ggtcatggaa cgctgtatgc cagcgatggc cgtacccgca
    30901 aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa tatatgcttt
    30961 ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc ttccacggcg
    31021 ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt ggcatggaac
    31081 cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc cgctatactg
    31141 aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg ctttagaagg
    31201 agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa catcaccgtc
    31261 aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa gaaggttgcc
    31321 acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct atggcaccgg
    31381 attctatcgt gatgattgag cagtgggaaa gcatcgcgca tcttgaagcg catctgcaaa
    31441 ccccgcacat gaaggcgtac agcgaagccg taaaaggcga cgtgctggag atgaatatcc
    31501 gcatcctgca acctgggatt taaacctgcc ttgtttgccc ggccatcctg accgggcaat
    31561 gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac cagttgcggc
    31621 gatcttccga gcgtttcttc gccagcagca tatccatcat cgcgtcagta cgctgatcgt
    31681 cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc gtggtttcac
    31741 gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc ggcttgcctt
    31801 tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc gcgtaataaa
    31861 cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg tgaccgtgtt
    31921 tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag cagcgtggca atgtgcagac
    31981 catcagagtc cgcatccgcg aggatacaga ttttgccata acgaagctgg ctcagatcgt
    32041 cgctgtcagg atcgataccg atcgctaccg aaatatcatg cacttcctgc gaggccagca
    32101 cttcgtcgga agaaacttcc caggtgttaa ggatcttacc tttcagtggc atgatcgcct
    32161 gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct tccacaagga
    32221 acagttcggt acggttaaga tcctgcgcgg tacagtcagc cagtttgcca ggcagtgctg
    32281 ggccgctggt cagctttttg cgcaccactt ttttagccgc acgcatacgg cgctgggcgc
    32341 tggaaatcgc catctccgcc agcagctccg ccgcctgaac gttctggttc agccacagga
    32401 taaaggcatc tttcaccacg cctgaaacga atgccgcgca ttgacgcgaa gagagacgct
    32461 ctttggtctg cccggcaaac tgcggatcct gcattttcac cgacagaaca taggcgcagc
    32521 gatcccagat atcttccgcc gacagcttca cgccgcgcgg cagaatgttg cggtattcac
    32581 agaactcacg catcgcgtcc aacaggccct gacgcagacc attaacatgg gtaccgccct
    32641 gcatcgttgg gataaggttg acgtagcttt cggtcagcag ttcaccgcct tccggcagcc
    32701 acagtagcgc ccagtccaca gcttcagtat cgccagcgaa attaccgata aacggttttt
    32761 ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga ccgtcctgat
    32821 agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca acgccggggc
    32881 acagtactgc tttggccttc agcacatgcg tcaggcgtga aacagaaaaa cgcgggctgt
    32941 caaagaaggt ttcatccggc cagaagtgca cgctggtacc ggtattgcgt ttaccgcaag
    33001 tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg ttataaacct
    33061 gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc accgaaatcc
    33121 ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta ccgcctgcat
    33181 gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga atatccaccg
    33241 gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaag attacatcca
    33301 cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt atcaataact tcttgcccca
    33361 aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc ggctcaagcc
    33421 cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa gttagcaatt
    33481 cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt gaaataatct
    33541 tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca ggaagcgtag
    33601 tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag cagccagatc
    33661 aaatccggcg cttccagcgg gtcaatctgc atgactttga gatcgtaaat atggcgtgac
    33721 tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata gtccgtcagc
    33781 agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa acattgtgac
    33841 aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga atcgccgcca
    33901 tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg cggcaactgc
    33961 ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa caagctcgct
    34021 tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga catcagtagc
    34081 cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc tcggtggtca
    34141 gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatagta tccagcgtaa
    34201 agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc aggcggcgac
    34261 cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat ttgacgtgcg
    34321 gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt tgatcgagcc
    34381 aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg cgttctggcg
    34441 catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca ccgtgcggca
    34501 cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata aacacgcgct
    34561 tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg aaatcgtggt
    34621 tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg aaatgctgat
    34681 aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg tcgaattcgt
    34741 gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg tttaccccta
    34801 acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa atcctgactc
    34861 tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct taggtttcac
    34921 gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc aaccagtccg
    34981 ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta ttaggataat
    35041 cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc accatcgcgt
    35101 catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg ggcgcagtct
    35161 gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc cgatattgtg
    35221 cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt aacaaacggc
    35281 gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca ggtgtgtaac
    35341 gcttcattta tgcccactca ttttttaacg cttgatgatg cagctgcagc cattgcaaag
    35401 cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat gcctgttccc
    35461 ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga ataccgcttg
    35521 cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg ccccccgggc
    35581 ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagtcccgcc tcttcaatcg
    35641 cttcgagacg ggcaacatct tccacacttt caccctcttc tatcatcccg gcaaccatct
    35701 ccagtagcca gggggtttca ctggtgtcgt atgcggcaat ccgaatctgc tcaatcagca
    35761 caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg cgctcaaaaa
    35821 tttccctccg tacctcatga ctcatttgcc cgttgaataa acgatgacga aatctataaa
    35881 gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct acatcgtttt
    35941 tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg gcactaatag
    36001 tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat gtgttaaatt
    36061 gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg gcttctgcta
    36121 gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac aaggaatgca
    36181 aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tatgggttca gttcgttgag
    36241 ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc cggaattgcg
    36301 taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc gcagtccatt
    36361 actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc gcgacgcgaa
    36421 cggcatcaac tctaacgcga ccagtgcgtc cctgcagtta actcaatcca tttttgatat
    36481 gtcgaaatgg cgtgcgttaa cgctgcagga aaaagctgca gggattcagg acgtcacata
    36541 tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca acgtgttgaa
    36601 tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc gtcaattaga
    36661 tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc agaacgcccg
    36721 cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc ttgataacgc
    36781 ggtagagcag ctgcgccaga tcaccggtaa ctactatcca gaactggcag cgctgaatgt
    36841 cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag aagccgaaaa
    36901 acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg agcaaattcg
    36961 ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg ggatttctga
    37021 cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg acgacagcaa
    37081 tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg gcggaatggt
    37141 taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc aactggaaag
    37201 cgcgcatcgt agcgtcgtgc aaaccgtacg ttcctccttc aacaacatta atgcatctat
    37261 cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat tagacgcgat
    37321 ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg caaccaccac
    37381 gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga ttaatcagct
    37441 gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac tgaacaatgc
    37501 gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc cggaacagaa
    37561 tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc aaacatccgc
    37621 acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac gacggggctt
    37681 cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt atctgccgca ttcttccccc
    37741 ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc actggtcccg
    37801 gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca aaaactggag
    37861 cgcgcgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc tggctggctg
    37921 tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt cagctgcaaa
    37981 cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag aagccgaacg
    38041 tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg aaggtcagtg
    38101 ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg cccagcaatc
    38161 cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc tgatgggcgg
    38221 cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca gcccggctta
    38281 cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag gccgcaccat
    38341 gaccgtaccg aagacagcca tggcaccaaa accggcgacc accactaccg ttacccgtgg
    38401 cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa ctggcacctc
    38461 ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga gcgcccggac
    38521 tggcgtgaga aagcccacga atacggtttc aattttcaca ccatgtacgg cgagccgtac
    38581 tggtgtgaag atgcttacta caagttgacc ctcgcccagg ttgaaaagct ggaagaagtc
    38641 accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc cagcgatgag
    38701 ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgcca gtcatggctg
    38761 acgcaccagc catcgcttta ttcgcgtctt gatctggcgt gggatggcac tggcgaacct
    38821 aaacttctgg aaaataacgc cgatacgcca acgtcactat acgaggcggc gttctttcag
    38881 tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag cgaccagttt
    38941 aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc tgcgtgaaca gtatggcttc
    39001 cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac cattcagtat
    39061 ttgcaggact gcgcaacgga agctgaaatt gctactgagt tcctctacat cgatgatatc
    39121 gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc caacctgttc
    39181 aagctgtatc cgtgggaatt tatgttgcgt gagatgtttt ccaccaagct ggaggatgca
    39241 ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaggc acttctaccg
    39301 ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt tgcggaagat
    39361 gatcatccgc aaatagaaaa atatgtggtt aaaccgatct tctcccgtga aggcgcaaac
    39421 gtgtcgatca ttgaaaacgg caaaaccatt gaagcagcgg aaggtccgta tggcgaagaa
    39481 gggatgattg ttcagcaatt ccacccgtta ccgaaattcg gcgacagcta tatgctgatt
    39541 ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga ccgtgcattg
    39601 atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc aatgatgccg
    39661 gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata ctcaggctgc
    39721 ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac gcacctaaga
    39781 catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca ccttcatggt
    39841 cgaggtaatt caccagagga tgctgctcta ctgccccttg ccacgtcaga ttcgctttca
    39901 catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc cacttcactg
    39961 tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca tctcgcagcg
    40021 ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg tcgatactca
    40081 actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc caggaaccgt
    40141 ggtcaaagcc ccaggcttct ttatccagcg tcaccgggac cggcgctaac agctcaacca
    40201 gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac agcgcctgcg
    40261 ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact cctgttccac
    40321 gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat gtcatcccca
    40381 acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga ctaccgtgac
    40441 ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact aaaggtgtca
    40501 ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa caatgatgat
    40561 gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag ttgtcgatgt
    40621 cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc gcgtttctcg
    40681 gcgttctcgg gcaaaaaccc tcgaaccgct tactggcgtt ttcgctagga tttgcggcgg
    40741 ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca gctgaaggaa
    40801 tgtcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctat tttggcctgg
    40861 accgcatgtt gccacatgcg catccgcagg atttaatgca aaaatcggtg cagccgttgc
    40921 caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg cataacttcc
    40981 cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga tttggcatcg
    41041 cactggccgt cgctttgcac aatatccctg aaggtctggc agtggcaggc ccggtttatg
    41101 cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga ctagcagaaa
    41161 ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg gtggtcatgg
    41221 cggcaatcat ggcggcggtt gcaggaatta tggtggcgct ctcggttgat gaattaatgc
    41281 cgctcgccaa agagattgac cctaataata accccagcta tggcgtacta tgtggaatgt
    41341 cagtgatggg attcagttta gtgctgctac aaacggcggg aattggttaa aaacaacatc
    41401 agaagttgtc cagcacatgg acaacttctg ccagcttcac gaaagcctta ttctaccttt
    41461 tcggctgtct cttctctcgt actgtttaag tatttgttcc gcgcttcaag cagtgcgtct
    41521 ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca ggaaataacc caccacaacc
    41581 agagcaatca atacaatcca gtaccaggcg ataaacatcc ttttaccctt aatatttaac
    41641 aatactaaac atttagcgca taaatttcac atatcctttt tcggatatat tcatatggtc
    41701 gcataataac ggaacaatta tatgattttg atcataatga aaaagaatgc ctgatattaa
    41761 tgaaattttc tcatagaaag agaagcggaa ttaacatctt gcatagcggg gcaaaataaa
    41821 aaccgaagcc agctacaact tcggtttctt gattaaggca gtaattaagc agcggttttc
    41881 agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg
    41941 ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
    42001 cattagtcag ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa
    42061 tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga
    42121 aaacgtggcc cgggcgattc agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa
    42181 cggtcgtaat acgatcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg
    42241 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcaa
    42301 gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg
    42361 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
    42421 cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat
    42481 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
    42541 aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc
    42601 caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct
    42661 aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct
    42721 cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat
    42781 aagcggattc actataacgc taatgattag cggcagcaac gcatagcttc acataattct
    42841 ggtttatgac ttacccttat cgcactacaa tggtactcaa cacctatcag tacagggaaa
    42901 ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc
    42961 ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag
    43021 ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc
    43081 tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag aatgagcgaa ctggaaaatc
    43141 gcagtactga gattaaaaag cagccagatc cagagactct ccccccaacg ctgtaaacat
    43201 ttgtctttaa tgcactgtaa attatgaatt aatcctctaa tttacagtgc tttctattac
    43261 cttatcgctt aattccattc agaatatcat cacaaaa
//
LOCUS       DAZFYC010000029        42105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFYC010000029 DAZFYC010000000
VERSION     DAZFYC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.029"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1733..3159
                     /locus_tag="ACHKW5_001772"
                     /pseudo
     CDS             1733..3159
                     /locus_tag="ACHKW5_001772"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3259..5178)
                     /gene="dhaR"
                     /locus_tag="ACHKW5_001773"
     CDS             complement(3259..5178)
                     /gene="dhaR"
                     /locus_tag="ACHKW5_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO7511182.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5406..6476
                     /gene="dhaK"
                     /locus_tag="ACHKW5_001774"
     CDS             5406..6476
                     /gene="dhaK"
                     /locus_tag="ACHKW5_001774"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO7511183.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSRVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6487..7119
                     /gene="dhaL"
                     /locus_tag="ACHKW5_001775"
     CDS             6487..7119
                     /gene="dhaL"
                     /locus_tag="ACHKW5_001775"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO7511184.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7130..8548
                     /gene="dhaM"
                     /locus_tag="ACHKW5_001776"
     CDS             7130..8548
                     /gene="dhaM"
                     /locus_tag="ACHKW5_001776"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO7511185.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            8868..10565
                     /gene="treA"
                     /locus_tag="ACHKW5_001777"
     CDS             8868..10565
                     /gene="treA"
                     /locus_tag="ACHKW5_001777"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7511186.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(10644..11084)
                     /gene="ycgY"
                     /locus_tag="ACHKW5_001778"
     CDS             complement(10644..11084)
                     /gene="ycgY"
                     /locus_tag="ACHKW5_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO7511187.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(11262..11516)
                     /gene="ymgE"
                     /locus_tag="ACHKW5_001779"
     CDS             complement(11262..11516)
                     /gene="ymgE"
                     /locus_tag="ACHKW5_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7511188.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGSDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            11717..12451
                     /gene="ycgR"
                     /locus_tag="ACHKW5_001780"
     CDS             11717..12451
                     /gene="ycgR"
                     /locus_tag="ACHKW5_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO7511189.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(12453..13064)
                     /gene="emtA"
                     /locus_tag="ACHKW5_001781"
     CDS             complement(12453..13064)
                     /gene="emtA"
                     /locus_tag="ACHKW5_001781"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO7511190.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            13164..14078
                     /gene="ldcA"
                     /locus_tag="ACHKW5_001782"
     CDS             13164..14078
                     /gene="ldcA"
                     /locus_tag="ACHKW5_001782"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO7511191.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            14173..15909
                     /gene="cvrA"
                     /locus_tag="ACHKW5_001783"
     CDS             14173..15909
                     /gene="cvrA"
                     /locus_tag="ACHKW5_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO7511192.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16295..17365)
                     /gene="dadX"
                     /locus_tag="ACHKW5_001784"
     CDS             complement(16295..17365)
                     /gene="dadX"
                     /locus_tag="ACHKW5_001784"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO7511193.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(17375..18673)
                     /gene="dadA"
                     /locus_tag="ACHKW5_001785"
     CDS             complement(17375..18673)
                     /gene="dadA"
                     /locus_tag="ACHKW5_001785"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO7511194.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            19003..20535
                     /gene="ycgB"
                     /locus_tag="ACHKW5_001786"
     CDS             19003..20535
                     /gene="ycgB"
                     /locus_tag="ACHKW5_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO7511195.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(20587..21306)
                     /gene="fadR"
                     /locus_tag="ACHKW5_001787"
     CDS             complement(20587..21306)
                     /gene="fadR"
                     /locus_tag="ACHKW5_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO7511196.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            21528..23069
                     /gene="nhaB"
                     /locus_tag="ACHKW5_001788"
     CDS             21528..23069
                     /gene="nhaB"
                     /locus_tag="ACHKW5_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO7511197.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            23215..23745
                     /gene="dsbB"
                     /locus_tag="ACHKW5_001789"
     CDS             23215..23745
                     /gene="dsbB"
                     /locus_tag="ACHKW5_001789"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO7511198.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(23791..25059)
                     /gene="umuC"
                     /locus_tag="ACHKW5_001790"
     CDS             complement(23791..25059)
                     /gene="umuC"
                     /locus_tag="ACHKW5_001790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO7511199.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(25059..25478)
                     /gene="umuD"
                     /locus_tag="ACHKW5_001791"
     CDS             complement(25059..25478)
                     /gene="umuD"
                     /locus_tag="ACHKW5_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7511200.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            25851..26761
                     /gene="hlyE"
                     /locus_tag="ACHKW5_001792"
                     /pseudo
     CDS             25851..26761
                     /gene="hlyE"
                     /locus_tag="ACHKW5_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(26968..27414)
                     /gene="ycgN"
                     /locus_tag="ACHKW5_001793"
     CDS             complement(26968..27414)
                     /gene="ycgN"
                     /locus_tag="ACHKW5_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO7511201.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(27506..28165)
                     /gene="ycgM"
                     /locus_tag="ACHKW5_001794"
     CDS             complement(27506..28165)
                     /gene="ycgM"
                     /locus_tag="ACHKW5_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7511202.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(28237..28530)
                     /locus_tag="ACHKW5_001795"
     CDS             complement(28237..28530)
                     /locus_tag="ACHKW5_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO7511203.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            28772..29173
                     /gene="pliG"
                     /locus_tag="ACHKW5_001796"
     CDS             28772..29173
                     /gene="pliG"
                     /locus_tag="ACHKW5_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO7511204.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(29293..29661)
                     /gene="ycgJ"
                     /locus_tag="ACHKW5_001797"
     CDS             complement(29293..29661)
                     /gene="ycgJ"
                     /locus_tag="ACHKW5_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO7511205.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            30181..30876
                     /gene="minC"
                     /locus_tag="ACHKW5_001798"
     CDS             30181..30876
                     /gene="minC"
                     /locus_tag="ACHKW5_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO7511206.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            30900..31712
                     /gene="minD"
                     /locus_tag="ACHKW5_001799"
     CDS             30900..31712
                     /gene="minD"
                     /locus_tag="ACHKW5_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO7511207.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            31716..31982
                     /gene="minE"
                     /locus_tag="ACHKW5_001800"
     CDS             31716..31982
                     /gene="minE"
                     /locus_tag="ACHKW5_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO7511208.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(32354..32698)
                     /locus_tag="ACHKW5_001801"
                     /pseudo
     CDS             complement(32354..32698)
                     /locus_tag="ACHKW5_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(32733..32852)
                     /locus_tag="ACHKW5_001802"
     CDS             complement(32733..32852)
                     /locus_tag="ACHKW5_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7511209.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(32813..32998)
                     /gene="ymgJ"
                     /locus_tag="ACHKW5_001803"
     CDS             complement(32813..32998)
                     /gene="ymgJ"
                     /locus_tag="ACHKW5_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO7511210.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            33099..33272
                     /gene="ymgI"
                     /locus_tag="ACHKW5_001804"
     CDS             33099..33272
                     /gene="ymgI"
                     /locus_tag="ACHKW5_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO7511211.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            33274..33618
                     /gene="ymgG"
                     /locus_tag="ACHKW5_001805"
     CDS             33274..33618
                     /gene="ymgG"
                     /locus_tag="ACHKW5_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO7511212.1"
                     /translation="MKKKILTFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            33622..33957
                     /gene="ymgD"
                     /locus_tag="ACHKW5_001806"
     CDS             33622..33957
                     /gene="ymgD"
                     /locus_tag="ACHKW5_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO7511213.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPDLSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(34014..36335)
                     /locus_tag="ACHKW5_001807"
     CDS             complement(34014..36335)
                     /locus_tag="ACHKW5_001807"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7511214.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(36452..36661)
                     /locus_tag="ACHKW5_001808"
     CDS             complement(36452..36661)
                     /locus_tag="ACHKW5_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511215.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(37061..37279)
                     /gene="ymgF"
                     /locus_tag="ACHKW5_001809"
     CDS             complement(37061..37279)
                     /gene="ymgF"
                     /locus_tag="ACHKW5_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO7511216.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(37411..38934)
                     /gene="pdeG"
                     /locus_tag="ACHKW5_001810"
     CDS             complement(37411..38934)
                     /gene="pdeG"
                     /locus_tag="ACHKW5_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7511217.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDIVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(39266..39514)
                     /gene="ymgC"
                     /locus_tag="ACHKW5_001811"
     CDS             complement(39266..39514)
                     /gene="ymgC"
                     /locus_tag="ACHKW5_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO7511218.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(39627..39893)
                     /gene="ariR"
                     /locus_tag="ACHKW5_001812"
                     /gene_synonym="ymgB"
     CDS             complement(39627..39893)
                     /gene="ariR"
                     /locus_tag="ACHKW5_001812"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO7511219.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(39922..40194)
                     /gene="ymgA"
                     /locus_tag="ACHKW5_001813"
     CDS             complement(39922..40194)
                     /gene="ymgA"
                     /locus_tag="ACHKW5_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO7511220.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(40237..40473)
                     /gene="ycgZ"
                     /locus_tag="ACHKW5_001814"
     CDS             complement(40237..40473)
                     /gene="ycgZ"
                     /locus_tag="ACHKW5_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO7511221.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            40787..41998
                     /gene="bluF"
                     /locus_tag="ACHKW5_001815"
     CDS             40787..41998
                     /gene="bluF"
                     /locus_tag="ACHKW5_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO7511222.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT        11080 a        10321 c         9833 g        10871 t
ORIGIN      
        1 ttttttaacc gctgtttgtt gtaatatttt tgccatattt atagtaacga attggaataa
       61 gcaacgacaa cttagggaat aactcgatat aaacccacct ttgtaagggg gatgttaaat
      121 atatattaat tcctgtgttc ctgattttgc ccggtaatag agaattattc tctgagaagc
      181 tcatcatgat tgcaccatac aaatatcatg atgataagag gatattattc attgatttaa
      241 acaaaatgac aaaatccata gacccatagt ctgggtacta tataggagtg gtatgggcat
      301 caaacaacac aatgggaata ccaaagcaga tcgtctcgct gaattaaaaa tccgttcgcc
      361 ctcaattcaa ctgataaaat ttggcgctat tggtttgaat gcaattatct tttcccccct
      421 gctgatagct gctgatacag gaagtcaata tggcaccaat attactatta atgatggtga
      481 cagaattaca ggagataccg ccgatccatc aggaaacctc tatggtgtaa tgaccccagc
      541 aggaaacacg cctggcaata tcaacctggg taatgatgtc accgtcaatg tcaacgacgc
      601 ctctggatat gcaaaaggaa tcattattca gggcaaaaac agctccctga cagctaaccg
      661 actcacagta gatgttgttg gtcaaacctc tgccatcggc attaacttaa ttggtgacta
      721 tacccatgct gacttaggca caggcagcac cattaagagt aacgatgacg gcatcattat
      781 tgggcatagc tcaacactaa cagccactca attcaccatt gaaaactcga acggtatagg
      841 cctaaccatc aatgactatg gcaccagtgt cgatcttgga agcggaagta aaatcacgac
      901 cgatggaagt acaggtgttt atatcggtgg tctcaacggc aataacgcca atggtgctgc
      961 gcgttttacg gcgacagacc tgacaatcga tgttcagggc tacagcgcca tggggataaa
     1021 cgtacagaaa aactctgttg tcgatctcgg aacaaacagt accattaaaa ccaatggcga
     1081 taatgcacac ggcctctgga gctttggcca ggttagcgcg aatgcactca ctgttgatgt
     1141 aactggagcc gcggccaatg gcgtcgaagt tcgtggtggt acaaccacta tcggtgcaga
     1201 tagccatatt tcttccgcgc agggcggtgg cctcgtcacc agtggttcag acgcgacaat
     1261 caatttttct ggcacggcag cgcaacgaaa cagcatcttt tccggcggtt cttatggtgc
     1321 ctcggcccag acggcaacgg ctgttgtcaa catgcaaaat accgatatta cagttgatcg
     1381 taatggcagt ctggcgctgg gtttgtgggc gctcagcggc ggtagaataa ccggagacag
     1441 tttggctatc accggcgcgg caggagccag gggaatttat gccatgacca acagccagat
     1501 cgacctcacg agcgatctgg tcattgatat gagtacaccc gaccagatgg ccattgcaac
     1561 gcaacatgac gatggttatg ccgccagccg catcaacgcc tcgggtcgta tgcttatcaa
     1621 cggtagcgtt ctttccaaag gtgggctaat caatctagat atgcacccag ggtcggtttg
     1681 gacaggttcc tccctcagcg ataatgtcaa tggcgggaaa ctggacgttg caatgaataa
     1741 cagcgtctgg aacgtaacaa gtaattctaa tctcgacacg ctggcgctga gccattcaac
     1801 tgtcgatttt gccagccacg ggtcaactgc cggcacattt gccacattaa acgtagagaa
     1861 cctgagcggt aacagtacct ttattatgcg tgctgatgtt gttggcgagg gtaatggcgt
     1921 taataataaa ggggatttat tgaatatcag cggaagtagt gctggtaatc acgtattggc
     1981 tatccgcaac cagggcagcg aggccactac gggaaatgaa gttctgacag tggtaaaaac
     2041 cactgacggt gcggcctcgt tcagcgcgtc ttctcaggtt gagttggggg gatatctgta
     2101 cgatgtgcgt aaaaatggca ctaactggga gctttacgct tccgggacag ttccggaacc
     2161 gactcctaat cctgaaccca caccagctcc cgctcagcct cccatagtca accccgatcc
     2221 tacgcctgaa cccgctccca cgcctaaacc caccacgacc gcagatgctg gcggcaatta
     2281 tctcaatgtc ggttacttat tgaactatgt tgaaaaccgt acgctgatgc aacggatggg
     2341 tgacctgcga aatcagagta aagacggtaa tatctggttg cgcagttatg ggggaagcct
     2401 ggactccttt gccagtggca aactgagcgg ctttgacatg ggttacagcg gtatccagtt
     2461 tggtggggat aaacgtctct ctgatgtaat gccgttgtat gtcggtctgt atattggctc
     2521 aacacatgca tcgccggact atagcggagg cgacggtacc gcacgttcag actacatggg
     2581 aatgtacgcc agttacatgg cacaaaacgg tttttacagc gatctcgtta taaaagcatc
     2641 gcgccagaaa aatagtttcc acgtactgga cagtcagaac aacggcgtta acgccaacgg
     2701 cactgcgaat ggaatgagca tctccctgga agccgggcag aggttcaacc tgtcccctac
     2761 tggttatggg ttctatatag agccgcaaac ccagcttaca tacagccacc agaatgagat
     2821 ggctatgaag gcgagtaatg gcctcaatat acatctgaat cactacgaat cgctgctggg
     2881 gcgtgccagc atgatactgg ggtatgacat caccgcaggc aacagccagc tgaatgtcta
     2941 tgtgaagact ggcgctatcc gcgagttttc aggggatacc gaatatctgt gaacaactcc
     3001 cgggagaagt acagtttcaa aggtaatggc tggaataacg gcgtgggagt cagtgcacag
     3061 tataacaaac agcacacatt ctatctcgaa gcggattaca cgcagggtaa cctctttgat
     3121 cagaagcaag tcaacggagg atatcgcttc agcttttaag tgcgggatag acaagccctt
     3181 aaacgatgct gaaaattgtt agcgtgcgcc ggaatgaaat acagatcact ctgatgtatc
     3241 agaaatcgtt tctgtctttc atacccggcg cttaaactgc cctgcatcaa tgccatgttg
     3301 cttcattttc cgccacagcg tagtgcggcc gatcccaagt aaagccgaca tttcctgaat
     3361 gcgaccgcct gtgacctggg ctgcgttaat aattgcctct ttttcaactt ccgcaaatga
     3421 cagactggtg gaaaggcggg tggcgctgac atcatctgtc gcctgctcgg taaacagatg
     3481 ttcaggtaaa tcactgacgc gaatgcgccc gttatcacta ctcagagcaa gattctcgat
     3541 gacgctgtaa agttcaaaat cgttgcctgg ccatgcacaa gaaaccaggc gagcgagggc
     3601 atcgtcatca attttcagcc gcgtagagaa gcgtttttca agactgcgta atttgttatt
     3661 caccagcgcc ggaatgctgc cacgccgcat acgcagaggc gggatggtaa tttcaaatgc
     3721 atgcagcgcg taatacagct ggcgactaaa acgattttgt tccaccagca ttgcgaggtc
     3781 cgcggtcgtt gttgcaatca ctttgacatc aattggtatt aaacgccgcg catccagtcg
     3841 cgtgataacc ccctgcttga taacctgaag caaagcagac tgtaactcca ccgccagata
     3901 ttcaatcttt tcaagaaaca gcgtgccgcc gtgtgccagt tccagccgac tcagacggcc
     3961 attttcattg tccgtgcgat cgccaccaat aaattcttcc gccagcgcag catcaccata
     4021 taactcacaa ttgacggcga tataaggacc tgcagcacgc tcgctttcat tatgaattgc
     4081 ctgacttagc agtgccttgc ccacgccctc ttctccacaa agcaggacag gaaagctact
     4141 gcgcgccgcc tggcgaccaa aatgaatcaa gcggcgggtt tgcggatcgt cctgtggcat
     4201 atgagcaaag gtatggctga cttttcctaa ttgactggtc atcaactgcc gcatctgttc
     4261 cacaggatgg agcaacaaaa taaagctggt tccctgcgtt tctattatcg gtttaagggt
     4321 tatcaccgca tcaataaact ggtgctggct ttcaaaggtt gcttctacgt gtttgagcgg
     4381 atgtgcctgt tttattgctt gttgcaatac ggcgggtaac gttaagagtt cagtgattgc
     4441 ccgtccctga cttgccgtcg cgtcaaggcg caagacccgc gccgcctggg cattaataaa
     4501 ttgcaaatta ccctgctcgt cccagctaat cacgccatca tccatacttt ctaacagggc
     4561 attaagttga tttaaatgac ggttagtttc agcgagcaaa ctgtccgtca gcagtaaatt
     4621 accgacctcg cgggcgattg ccaacgtcaa cggcaaatca gctgcggtag tttgctcaac
     4681 cggacacgcc agcgctattg ttcccgtcaa tcggcccttg ctgtcaaaca acggcgttgc
     4741 acaaaaggcc cagttccaga gtgcctgttt gaaatgttga tcggccatcg ttttcacggc
     4801 ctgaccagag atagccgcta acgatagcgc acaagtacca ataattccct cggcgcaata
     4861 cgtgccgtca ttgaatccca gtgcacttag ctgctgcaag gtttgcggat cgccattacg
     4921 gctgagaatg caggcggttt catcgaggat aagcagcgca cactctcgcg gtgccatata
     4981 ttcccaggca tcttccagcg cagcctgacc gagcgtcagc atcgctttct tacgccgata
     5041 aatagaagca aatgtcacgc cctgggcctg atgtggtacg ttccatgtct cccgtttcat
     5101 cagcttattg catcgctccc aggaggttgc aattaaggga gatatgcccc gaccatcgtt
     5161 gttaaaagcg ccactcatat cccgcatccc ttatgacaaa aggtgttccg gctcgcaatt
     5221 ttaagtatta atacacgtaa aagtttcaat atggaacatt cattaaagca tatgtttcac
     5281 tatgaaacat aatttgggct aattttcttt tcccttgcct gatgcacaat ggattcatcg
     5341 gcagtcagtg gtcgccgtgt cgttgaacat catccatgcc ctaccgtaat tgctggagca
     5401 aaataatgaa aaaattgatc aatgatgtgc aagacgtact ggacgaacaa ctggcaggac
     5461 tggcgaaagc gcatccatct ctgacactgc atcaggatcc ggtgtatgtc acccgagctg
     5521 atgcccccgt tgcaggaaaa gtcgccctgc tgtcgggtgg cggcagcgga cacgagccga
     5581 tgcactgtgg ttatatcggt caggggatgc tttcgggggc ctgtccgggc gaaattttca
     5641 cctcaccgac gcccgataaa atctttgaat gcgccatgca aattgatggc ggcgaaggtg
     5701 tactgatgat tatcaaaaat tacaccggcg atattcttaa ctttgaaaca gcgaccgagt
     5761 tactgcacga tagccgcgta aaagtgacca ctgtggtcat tgatgacgac gttgcggtaa
     5821 aagacagtct ttatactgcc gggcgacgcg gcgttgccaa caccgtatta attgaaaaac
     5881 tcgtaggcgc agcggcggag cgtggcgact cactggacgc ctgtgcggaa ctggggcgta
     5941 agttgaataa tcaaggccac tcaataggta tcgctctcgg tgcctgtacc gttcctgccg
     6001 cgggcaaacc ttcttttacc ctggcggata atgagatgga gtttggcgtc ggcattcatg
     6061 gtgagccggg tattgaccgc cgctccttct cttcccttga tcaaaccgtc gatgaaatgt
     6121 tcgacaccct gctggaaaat ggctcatacc atcgcacttt gcgtttctgg gattatcaac
     6181 aaggcagttg gcaggaagaa caacaaacca aacaaccgct ccagtctggc gatcgggtga
     6241 ttgcgctggt taacaatctt ggcgcaactc cgctttctga gctgtacggc gtctataacc
     6301 gcctgaccac acgttgccag caagcgggat tgactatcga acgtaattta attggcgcgt
     6361 actgcacctc actggatatg accggtttct caatcacctt actgaaagtt gatgacgaaa
     6421 cgctggcact ctgggacgcc ccggtccaca ccccggccct taactggggt aaataaggag
     6481 aaagcaatgt cactgagcag aactcaaatt gttaactggc tcactcgttg tggcgatatt
     6541 ttcagcaccg agagcgagta tcttaccgga ctggatcgcg aaattggcga cgctgaccac
     6601 gggctaaata tgaaccgagg ctttagcaaa gtggtggaaa aactccctgc tatcgcagat
     6661 aaagatatcg gtttcattct caagaatacc ggtatgacgc tgctttccag cgtcggtggt
     6721 gccagtggtc cgctgttcgg taccttcttt atccgcgccg cacaggcgac ccaggcacgg
     6781 caaagcctga cactggaaga gctttatcag atgttccgcg atggcgtgga cggcgtaatc
     6841 agtcgcggga aagccgaacc tggcgataaa accatgtgtg atgtgtgggt gccggtggtg
     6901 gaatcgttac gtcagtccag cgagcaaaat ctctctgttc cggtggcgct cgaagctgcc
     6961 agtagaatcg ccgaatccgc tgcacaaagt acgattacga tgcaagcccg caaaggccgc
     7021 gccagttatc tcggtgaacg cagtattggt caccaggatc ccggcgcgac ctcggtgatg
     7081 tttatgatgc aaatgttggc gttagccgca aaagagtaag gaattggtga tggtaaacct
     7141 ggtcatagtt tcacatagca gccgactggg agaaggtgtc ggtgaattag cccgtcagat
     7201 gttaatgagt gatagttgta aaatcgccat tgccgcggga attgacgatc cacaaaatcc
     7261 tattggtacc gatgccgtca aagtgatgga ggccatcgaa tctgttgctg atgccgacca
     7321 tgtgctggtc atgatggata tgggtagcgc attattgagt gctgaaactg cgctggaatt
     7381 gctggctccc aagatcgccg caaaagtacg tttgtgtgct gcgccgttgg tcgaaggtac
     7441 actggcagca acggtcagcg cggcctcggg agcggatatc gacaaagtta tctttgacgc
     7501 tatgcatgcg ctggaagcca aacgtgaaca actgggttta ccgtcctccg acactaaaat
     7561 ctctgacaca tgtcctgcgt acgatgaaga agcccgttct ctggcggtgg tcataaaaaa
     7621 ccgtaacggc ctgcatgtac gtccggcctc ccggctggtt tataccttat cgacatttaa
     7681 tgccgatatg ttgctggaaa aaaacggcaa atgcgtcaca ccagagagta ttaaccagat
     7741 tgcgttacta caagttcgct ataacgatac gctgcgcctg attgcgaaag ggccagaagc
     7801 tgaagaggca ctgatcgctt tccgtcagct ggctgaagat aactttggtg aaacggagga
     7861 agtcgctcca cctactctgc gtcccgttcc gcctgtttcg ggtaaagcct tttattatca
     7921 accagtttta tgtacggtac aggcaaaatc aaccctgacc gtggaagaag aacaagatcg
     7981 attacgccag gctattgact tcacgttatt agatctgatg acgttaacag caaaagcaga
     8041 agccagcggg cttgacgata ttgccgcaat cttttctggt caccatacac tgttagatga
     8101 tccggaactg ctggcggcgg caagcgaact ccttcagcat gaacattgca cggcagaata
     8161 tgcctggcag caagttctta aagaacttag ccagcaatac cagcaactgg atgatgaata
     8221 tctacaagct cgctatattg atgtggacga tcttctgcat cgcaccctgg tccacctgac
     8281 ccaaacgaaa gaagaactcc cgcagtttaa ctcgccaact attctactgg cggagaacat
     8341 ttatccttcc acagtactgc aactggatcc ggcggttgta aaaggtatct gccttagcgc
     8401 cggaagtccg gtatcccaca gcgccctaat cgcccgtgaa ctggggattg gctggatttg
     8461 ccagcagggt gagaaactgt atgcgataca accagaagaa acgctaacgc tggacgttaa
     8521 aaggcaacgt ttcaaccgtc agggttaagc cactgttcgg atggcatcgt tctgatgtca
     8581 tccggcaatt tactcagcac taatggtttt taactacctg ttatattttt caccaattta
     8641 tgatgatttt tcttcgctaa tcacgaaccc aacgccgctg gctggctaat aaacagacgt
     8701 tatttgatca ctctatttcg ccgttattta tccctttaat tcccttttct aaaatgcctg
     8761 acagttcgca gaatgagatt tcgatcatgc agctagtgcg atcctgaact aaggttttct
     8821 gatacttgaa taccgtttta ttcggtttcg ccaaaggaga atgattgatg aaatcccccg
     8881 caccttctcg cccgcaaaaa atggcgttaa ttccagcctg tatctttttg tgtttcgctg
     8941 cgctatcggt gcaggcagaa gaaacaccgg taacaccaca gccgcctgat attttattag
     9001 ggccgctgtt taatgatgtg caaaacgcca aactttttcc ggaccaaaaa acctttgccg
     9061 atgccgtgcc gaacagcgat ccgctgatga tccttgctga ttatcggatg cagcaaaacc
     9121 agagcggatt tgatctgcgc catttcgtta acgtcaattt caccctgccg aaagaaggcg
     9181 agaaatatgt tccgccagag gggcagtcac tgcgcgaaca tattgacgga ctttggccgg
     9241 tattaacgcg ttctaccgaa aacaccgaaa aatgggattc tctgttaccg ctgccggaac
     9301 cttatgtcgt gccgggcgga cgttttcgcg aggtatatta ctgggacagt tacttcacca
     9361 tgttaggact tgccgaaagc ggtcactggg ataaagtcgc ggatatggtg gccaattttg
     9421 ctcatgaaat agacacttac ggtcatattc ccaacggcaa ccgcagttac tatttaagcc
     9481 gctcgcaacc gcccttcttt gccctgatgg tagagttact ggcgcagcat gaaggcgatg
     9541 ccgcgttgaa gcaatacctg ccgcaaatgc aaaaagaata tgcttactgg atggacggtg
     9601 ttgaaaacct gcaagccgga caacaggaaa aacgcgttgt aaaacttcag gatggtaccc
     9661 ttctcaaccg ctactgggac gatcgcgata cgccacgacc agagtcatgg gtggaagata
     9721 ttgccaccgc caaaagcaat ccgaatcgac ctgccactga aatttaccgc gacctgcgct
     9781 ctgccgctgc gtctggctgg gatttcagct cgcgctggat ggacaacccg catcaactaa
     9841 ataccttacg caccaccagc atcgtaccgg tcgatctgaa cagcctgatg tttaaaatgg
     9901 aaaaaatcct cgcccgcgcc agcaaagctg ccggagataa cgcgatggca aaccagtacg
     9961 aaacgctggc aaatgcccgt caaaaaggga tcgaaaaata catgtggaac gatcaacaag
    10021 gctggtatgc cgattacgac ctgaaaagtc ataaagtgcg caatcagtta accgcggccg
    10081 ccctgttccc gctgtacgtc aatgcggcag cgaaagatcg cgccaacaaa atggcgacgg
    10141 cgacgaaaac acatctgctg caacccggcg gcctgaacac cacgtcggtg aaaagtgggc
    10201 aacaatggga tgcgccaaat ggctgggcac cgttacagtg ggtcgcgaca gaaggattac
    10261 aaaactacgg gcaaaaagag gtggcgatgg acattagctg gcacttcctg accaatgttc
    10321 agcacaccta tgaccgggag aaaaagctgg tggaaaaata tgatgtcagc accaccggaa
    10381 cggggggcgg cggtggcgaa tatccattac aggatggctt tggctggacc aatggcgtga
    10441 cgctgaaaat gctggatttg atctgcccga aagagcaacc gtgtgacaat gttccggcga
    10501 cgcgtccgac cgttaagtca gcaacgacgc aaccctcaac caaagaggca caacccacac
    10561 cttaaccagc gcttactccg acaacgtcat tcggcttctc cggatggcgt tgtcggcaga
    10621 taacaccata gctatggttt tttctacaca gacggttttt caagtcctgg cgggaagtat
    10681 tgcgcaaatg tttgccacac gtggttgccg aagatagcgt catttctacg ggtaagtgtt
    10741 tcactttcat cacccggcga tttacgccga cgttggctat ataaagcatc aattgctacc
    10801 tgaatattgt tacggtgttc actcatcaac gtttttttgc tttctggagt taaattaata
    10861 atcacataat ccagacactc cagagaattt agcaaccaaa aaaagaattc agaccattcc
    10921 tcttcgcgta actgaagcat tgccgaacgg gtattttggc ttaatctgcc agaaggattg
    10981 atcaattcta aatgttcaat aagcgcagcc aaaaccgcct cactatccgc cccattttta
    11041 acgcctgact ggctactgtc taaaatattt attttctgaa tcacaccaaa aacaacgaaa
    11101 caataattaa aaaaggattc tgttgttgtt accgggaacc gactttatcg gatatcgaag
    11161 tgctatattg atttttttca cccgtaaagc aggtgtcgag tatacgttct gatgtgaaag
    11221 cggatagcgg catctgtatc gctatccgcc ttatgaaaat cttattctct tcgcaggagg
    11281 cggaatatgc ccagaacgag aatagctccc accaccgcca ccaggaagct gtgcagatta
    11341 aaaccactga tggagccgcc aatgccaaac atggtcgcca gccagccgcc gaccaccgca
    11401 ccgactatcc cgagaataca ggtcaggaaa aatccaccac catcactccc cggcatgatt
    11461 agcttggcga taatgccggc tatcaggcca aaaataatcc aggcaataat tcccatgttc
    11521 aggccctcgt ttcgatgaat cggacgcaag aaatgcttac atcaacgctt taagtatagg
    11581 caagggcggg atcgatgctg ggcgtatgtc ggctgattta agaacaacat gtaaaaaata
    11641 ttcacgcatt tgtattaagt tttgttaact gtgaccgata aaccaaagac agtttgtcag
    11701 tcaggagttt ttccgcgtga gtcattacca tgagcagttc ctgaaacaaa atccgttagc
    11761 cgtcctgggc gtgttacgcg atttgcacaa agccgcaatt cctttgcgtc tcagttggaa
    11821 tggcgggcag ctgatcagca aattactggc aataaccccg gataaactgg tgctggattt
    11881 cggcagtcaa gccgaagaca acatcgccgt gctaaaggca cagcacatta ccattaccgc
    11941 cgaaactcag ggtgcgaaag tcgagtttac tgttgaacaa ctacagcaga gtgaatactt
    12001 gcagcttccg gcatttatca ccgtaccgcc tcccacctta tggtttgtac aacgacgccg
    12061 atatttccgc atctccgccc cactccatcc gccttatttt tgccagacca aactggcgga
    12121 taacagtacg ttacgtttcc gcctgtatga tttgtcgtta ggcggcatgg gcgcattact
    12181 ggaaacagca aagcctgccg aattacaaga aggcatgcgc ttcgctcaga ttgaagtcaa
    12241 catggggcaa tggggtgttt ttcactttga cgcccagtta atctccatca gcgagcgcaa
    12301 agtgattgat ggcaagaatg aaaccatcac cactccccgt ctgagcttcc gttttcttaa
    12361 cgtcagcccg acggtggagc ggcaattaca gcggattatt ttctctctcg agcgagaagc
    12421 ccgagaaaaa gcggacaaag tgcgcgactg atttacatcg cgtccagtgc ctgctcaagt
    12481 ttgtagatat agcgcggagc ctgcggcgca ggatgatttc gcgctacgtg atcgaggaac
    12541 tcgtcagcat ccaaatcgtt gattttgctg atcgcctttt tccgatctga cgagaaagtc
    12601 cgtagcagcg cacctgcccc gttagcgtat gacaccacca gcgcatattg cagtaccttc
    12661 ggatcttcaa tgcctgccag cgggccggtt tccagaatat tcaggtaagc cgcccccatt
    12721 gaaatattac gctctggatt tttcagctcg ctggtcgtcg gctcaccact ccagcccata
    12781 cggcgataaa catcacgtcc ggaggttgaa gcttttaact gcatcaaacc aatggcattc
    12841 gatttactca ccgcgttcgg attaccaccc gattcaatag cgataatcgc cgtgatcaat
    12901 tgtggatcga cgccccaggc tgcaccggct ttctggctta ttggcatcca ctgcatcgca
    12961 cgttgcaccg gaactttcgc gttccacggc gggttcgtat agtcatgctt tgatgaacaa
    13021 cccgctaata acacaatcaa aaaggcaaac catctcaatt tcacttcttc tatccttata
    13081 gcctggaatc gccgttgagg tgacaacggc acacctaagc ggcatgatat acatttcgtt
    13141 tctgttgtca gcaaggaatt gccatgtctc tgtttcactt aattgcccca tcgggttact
    13201 gcattaaaca gcacgccgcg ctgcgtggta tccatcgcct taccgacgag gggcatcagg
    13261 tcaataacgt cgaggttatt gcccgtcgtt gtgagcgttt tgcgggtacg gaaacggagc
    13321 gcctggaaga tctcaattcc cttgccagac tgactacccc caacaccatc gtgctggctg
    13381 tacgcggcgg ttacggtgcc agtcgtttac tggcagatat tgactggcag gcactggtgg
    13441 ctcgccaaca acatgacccg ttactcattt gcggacatag cgattttacc gccattcagt
    13501 gcggtcttct ggcgcaaggc aatgtcatca cctttagcgg ccccatgctg gtagcaaact
    13561 ttggtgcgga tgaactgaac gcctttaccg agcaccattt ctggctggcg ttacgcaata
    13621 aaaccttcac cattgaatgg caaggggaag gcccgacatg tcagaccgaa ggcacgttgt
    13681 ggggaggcaa tcttgcgatg ctgatttcac tgattggtac accgtggatg ccaaaaattg
    13741 agaacggtat tctggtgctg gaagatatta acgagcaccc tttccgcgtc gaacgtatgc
    13801 tgttgcagct ctatcatgcg ggaattttgc cacgtcagaa ggcgattatt ctcggtagct
    13861 ttagcggcag cacgcccaat gattatgacg caggttacaa cctggagtca gtgtacgcgt
    13921 ttttgcgttc ccgcctgtcg attccactca ttaccggcct cgattttggt catgaacaac
    13981 gcacggttac gctgccgttg ggcgcacatg ccattctgaa taacacccgg gaaggcactc
    14041 agttgacgat tagcggtcat cctgttctta aaatgtaaaa aaatcacgct tcagggctaa
    14101 gtaaaacgct gtctctgccg ctaatatggt agggtttata taagagacag cgtaatcagg
    14161 agtaaccgac cgttggatgc cacaacaata attagccttt tcatcttagg atccatcctt
    14221 gtcaccagca gtatattact tagttcattt tcttcccgtc ttggcattcc tattctggtt
    14281 atttttctgg cgatcggcat gctggcagga gtcgatggcg tcggcggtat cccgtttgat
    14341 aattacccct tcgcctacat ggtaagtaac ctggcactgg cgattatttt gctcgacggc
    14401 gggatgcgca ctcaggccag ttcctttcgt gtggcgttag gaccggcact gtcgctggcg
    14461 acgctgggcg tgcttatcac ctctggttta accggcatga tggcggcgtg gctgtttaat
    14521 cttgatttga ttgaaggctt attaatcggc gctatcgtcg gctctaccga tgctgcagcg
    14581 gtcttttctc tgctgggtgg taaggggctt aacgaacgtg ttggctcgac gctggaaatt
    14641 gaatccggca gtaatgatcc aatggcggtc tttctgacga ttaccctgat tgcgatgatc
    14701 cagcaacatg aaagcagtgt cagttggatg ttcgtggtcg atattctgca gcaatttggc
    14761 ctcgggattg tcatcggact tggcggtggt tatttactgc tgcaaatgat taatcgcatc
    14821 gccctgcctg ccggattata tccattgctg gcattaagcg gcggtatttt aatttttgct
    14881 ttaaccacgg cgctggaagg cagcggcatt ctggctgttt atctgtgcgg ttttctgctg
    14941 ggtaatcggc cgattcgcaa ccgctacggc atcctgcaaa atttcgacgg cctcgcctgg
    15001 ctggcgcaaa tcgccatgtt cctggtgctg gggctattgg ttaacccaag cgatctgctg
    15061 cctattgcca ttccggcgct cattttgtcg gcatggatga tattcttcgc ccgtcccctt
    15121 tcggtatttg ctggattgct ccccttccgc ggtttcaatc tgcgtgagcg cgtgtttatc
    15181 agctgggtag gattacgcgg cgcggtgcca atcatcctgg ccgtgttccc gatgatggcg
    15241 gggctggaga atgcacgtct gttctttaat gtcgccttct ttgtggttct ggtttcactg
    15301 ctcttgcagg gaacatcact ctcgtgggcg gctaaaaaag ccaaagtggt ggttccgcca
    15361 gtgggacgtc cggtgtcacg cgttggcctg gatattcatc cggaaaatcc gtgggagcag
    15421 tttgtttatc aattgagtgc cgataaatgg tgcgtgggcg cggcactgcg cgatttgcat
    15481 atgccaaaag agacgcgtat tgcggcactg tttcgtgata accagttgct tcatcctacc
    15541 ggcagcaccc gactgcgcga aggcgatgtg ttgtgtgtaa ttggtcggga acgcgatctt
    15601 ccggcgctcg gtaaactgtt cagccagtcg ccgccggtcg cgctggatca acgcttcttt
    15661 ggtgacttca ttctcgaagc cagcgccaaa tatgctgatg ttgcgctgat atatggcctg
    15721 gaagacgggc gagaatatcg tgataagcag caaacgctgg gtgaaattgt ccagcagttg
    15781 ttaggcgcgg caccggttgt cggtgaccag gtagagtttg ccgggatgat ctggacggtg
    15841 gccgagaaag aagataatga agtgttgaag attggcgtac gggtggctga agaggaagct
    15901 gaatcttaaa tttaatgaag catctatcgc cggttgcgat gctttgctga acgcagcctg
    15961 agcctgcaat tgtttgtcgt ccataattta accttcattt gatgttgaat tgaacatatc
    16021 aaaatcaggc aattacacaa atctatgtac aggctccatc aagggtaaag cgtgatttat
    16081 ctgaagtcga gttcgagtca acaatgaagc gtaagcgttt tatgtagaac gcctgtgcgg
    16141 tgagttcagg ttccggggaa atagtgtcca ttcttttaat ggacactatc atgggcaatg
    16201 gctctgtcac attaacaact acagttgctg accagccgga tggatgattt tttcgcaacc
    16261 agaagacgct gtctccgctc cggcttacaa caagttacac tgtcacaacc gggacgcgta
    16321 acgccagcgc gcacatcaac tcatagccca ccgttccggc agcggcggcg acatcatcaa
    16381 ttttgatctc cttgccccac agctcaaccg gcgtaccaat ccccgcctgc gggcaaggcg
    16441 ttaaatcgac cgccagcata tccatcgaga cggtccccac cgtcatggtg cgcacgccgt
    16501 ccactaaaac aggggtaccg gtaggcgcgt ggcgcggata accgtcggcg taccctgcgg
    16561 cgacaatgcc gattcgctgt tcatcgcgcg cagtatagcg accgccgtag cccacacgct
    16621 cacccgcttt tagcgtctgg acaccaataa tctcactgct tagcgtcatc accggacgta
    16681 atccggtatt ggcgatatca cgccactgac cggacggcga agcgccatac aaaataatgc
    16741 caggccgaac ccagtcaaaa tgcgcttccg ggtgccacag agtcgccgcc gaattggaca
    16801 acgaacgccg acactccagc ccttccgccg cctgctcaat acgcgccatc gcgctggaaa
    16861 ttccatcagg atgttccgct tcggcaaaat gcgacatcag ggtcatctcg ccaacattcg
    16921 ccattgcccg caactgctgc cagacggtaa gcacgcgatc gggctggaag cccaaccgat
    16981 tcatcccact gttcacttta agataaatat ccaacggtgc ttttagccgc gcattttgca
    17041 gtgctttgag ctgccagttg ctgtgtacgc aggtggtcag gcggtgctgg tcataaatct
    17101 ccagatcctg agcatggaaa aatccttcca gcatcaggat cggccccttc cagccgcgct
    17161 cacgtaacgt tattgcctct tccaggttaa gtaatgcaaa gccatcggtg gccccgagcg
    17221 cgctccagat acgctcaata ccatgcccgt aagcgttcgc ttttaccacc gaccagacgc
    17281 gcgcgtgcgg cgcggcctgg cggacaatgg acagattctg ttttaatgcc tgcagatcga
    17341 ggctggcctg tatcggacgg gtcatctcgt ttccttagct gtgtgcgcca tgtaaatggc
    17401 ccggacgtga tggcgtaaat ccacggctgt agcgcgctac gcttagatcc tcatatggga
    17461 tcgctggcgt gcgaccagag agcagatcgc ttaacaactg accggaaccg caagccatcg
    17521 tccagccgag cgtgccgtga ccggtattca gccacagatt tttaaagcgt gtacgcccga
    17581 caaccggcgt accgtctggc gtcatcgggc gcaaacccgt ccagaaagtc gcctgctcga
    17641 catgaccgcc gcgtggatag agatcgcgaa ccaccatttc cagcgtttca cgacgcggtt
    17701 gcaacagctc ggtgttaaaa ccaacaattt ccgccattcc gccaacacga atgcggttat
    17761 cgaaacgggt aatggcgatt ttgtaggttt catcaagaat agtggatact ggcgcaccat
    17821 cttcctgcgc aattggaata gtcagcgagt agcctttcag cggatacacc ggaatatcaa
    17881 caatgccttt gagcatcgcc gtcgagtaag aaccaaacgc catcacatac gcatcggcct
    17941 taatcacttc atcgccacac ttcacgccgt agatttgctc gccgtcgcaa agcagttggt
    18001 caacgggcgt attaaagcgg aatttaaccc ccgcctgctc tgccatccgc gccagattct
    18061 gggtaaacag ctggcagtct ccggtttcat cattgggcag ttgcagacca cccgtcagtt
    18121 tgtgcgctac ttctgccagc gcgggctcca cttccgccag gcggctggat tccagcagct
    18181 gatacggtac gccggcatct tccagcacgg cgatatcgcg ggtcgcattt tcatactgtt
    18241 gttcggtacg gaacagttgc agcgtcccac cctgacgccc ttcatactga atattggttt
    18301 cggcgcgcaa tgctttcagg caatcacggc tgtattccgc cagacgcacc atccgccctt
    18361 tgttttccat gtagtggctg gtgtcgcagt tacgtaacat ttgccacatc catttcaact
    18421 gaaactgcgt accgtcgaga cggaccgcca gcggcgcatg gcgctggaac atccatttga
    18481 tcgcttttaa aggcacacct ggtgccgccc acggtgcagc atatccgggg gagatttgcc
    18541 cggcattcgc ggcgctggtt tccagggcgg cccccggctc ccgatcaatg acggtgacct
    18601 catgtcctgc ctgatttaag taccaggcgc tggcaacgcc taccacacca cttcccagta
    18661 tgacaactcg catagccact ccgataacgg tagatacagt aaaagaataa taatctaatt
    18721 acatcttgat aactcagttg aaaatattat tcaacatacg gcttttttat ggtgagctgg
    18781 ctcacatctc cctgactctg cggcaatatg gcgtctttag tatctcctgc tgaacgccct
    18841 ttttatccag cataaaattc cgttcagaag cggattagtg gcactctgat aataagaaat
    18901 cgcaaagaaa aaaattctcc ggcgacgcgg tttttaacag gtgttctatg cttgaaatga
    18961 ggtgctccac acagagagcg ccgacaacaa tgaggtgcgc gtatggcgac gatcgattct
    19021 atgaataagg acaccacacg tttgagcgat ggacccgact ggacgttcga cctgctggat
    19081 gtttatctgg cagagataga ccgggtggcg aaactctacc ggctggatac ctacccgcac
    19141 cagattgaag tgataacctc agaacagatg atggatgcct actccagcgt cggcatgcca
    19201 attaactatc cgcactggtc attcggtaaa aagtttatcg agactgaacg gctgtataag
    19261 cacggtcagc aaggactggc ctatgaaatc gtcattaact ctaacccgtg tatcgcttac
    19321 ctgatggaag agaacaccat taccatgcag gcgctggtga tggcacacgc ctgctatggg
    19381 cataactctt tctttaaaaa caattactta ttccgcagtt ggaccgacgc cagttcgatt
    19441 gtcgattatc tgatttttgc ccgtaaatat attaccgagt gcgaagaacg ttatggcgtt
    19501 gatgaagtag aacggcttct ggactcgtgc cacgcgctga tgaactacgg cgtggaccgg
    19561 tacaaacgcc cgcaaaaaat ctcgctgcaa gaagagaaag cccggcagaa aagtcgcgaa
    19621 gagtatctac aaagtcaggt caatatgctc tggcgtaccc tgccgaagcg cgaggaagag
    19681 aaaacggttg ctgaagcgcg ccgctatccg tccgaaccac aagaaaacct gctctatttt
    19741 atggagaaaa atgcgccact gctggaatca tggcagcgtg aaatcctgcg tattgtgcgt
    19801 aaggtgagcc agtattttta tccgcaaaaa cagactcagg tgatgaacga aggctgggcg
    19861 accttctggc actataccat ccttaaccat ctgtatgatg aagggaaagt aacggaacgt
    19921 tttatgctgg agtttttgca cagccacacc aatgtggtct tccagccccc ctataacagc
    19981 ccgtggtaca gcggcatcaa cccatatgcc ctcgggttcg ccatgttcca ggatattaaa
    20041 cggatttgtc agtcgccaac ggaagaagac aaatactggt tcccggatat cgccggttcc
    20101 gactggctgg aaacgctgca tttcgcgatg cgtgatttca aagatgagag ttttatcagc
    20161 cagtttctgt caccgaaagt gatgcgtgat ttccgcttct tcaccgtgct ggatgatgat
    20221 cggcataatt atctggagat ttccgctatt cataatgaag aaggttatcg ggagatccgt
    20281 aaccggttat cgtcgcaata taacttaagt aatctggagc cgaatattca gatctggaac
    20341 gtggatttgc gcggcgaccg ttcgctgacg ctgcgttaca ttccacataa tcgcgcaccg
    20401 ctggatcggg ggcgcaaaga agtgctgaag catgtgcatc gcctgtgggg atttgatgtg
    20461 atgctcgaac agcaaaacga agacggcagc atcgagttgc tggaacgttg cccgccaaga
    20521 atgggaaatc tgtaaaaaca acaaaaaacc cctcgtttga ggggtttgct ctttaaacgg
    20581 aagggattat cgcccctgaa tggctaaatc acccggcaga tttttctgca tccggtgcca
    20641 aatctcgcca ctctcatgcc catagcgacg cactgtttcg tacacctgat cgtgcgcgcc
    20701 ttcactgcac aacgccgaca gtttgtggta gaagcccagc gccagactgc gcgcttccgg
    20761 attggcgaaa tagtgacgac caatgcgcgt atacagccct ttcatcccgt taaggatcag
    20821 accgtaaatc gggttgccgg aagcaaacgc caggccgcgg aatatgttgt aatccagctc
    20881 ggcaaaggca tcggcgtgat cggccacttc attagcggta gccagcactt cctgcgcttt
    20941 atcgggatgc tgacgaaacg cggtgcgaat aaaaatagtg gaaatattgg tacgcaccga
    21001 cagcaaatta tcaataagct gcggcacact ttcgtgatcc agtcgcgcca gtgtttcaag
    21061 gatatttaaa ccagaggttt cccagaaatt attcaccttc gtcggcttgc catgttgaat
    21121 ggtcaaccag ccatctcgtg ccagacgctg taacacttca cgtaacgtag tacgcgttac
    21181 gccaattaat tctgaaagtt cacgttctgc gggcaaaata gtcccgggag ggaagcggtt
    21241 attccagata ctttcaataa tgtactcttc cgcgaaaccc gccgggcttt gcgccttaat
    21301 gaccatagtg agatttccat aacacagcaa aacaaagttg gactcatcat accagagggc
    21361 taactacgct gatagcagac aaaaagaaga aaaaagggat caaggcttca ttttccgcat
    21421 ctctttcccc ttgactatca agacttgcct cactcctgcc tgaccgttaa gctttgcgtt
    21481 taaacataaa aacatcattg attatttgta aggtaaggga aaccatcatg gagatctcct
    21541 ggggccgcgc gctatggcgc aattttttgg gccagtcccc cgactggtac aaactcgccc
    21601 tcatcatttt cttaatcgta aacccgttaa ttttcctcat cagccctttc gtcgcgggct
    21661 ggttgctggt agcggaattt attttcactc tggcgatggc cctgaaatgc tacccgctgc
    21721 tccccggtgg tctgttggct atcgaagcgg tattcatcgg catgaccagc gcggaacacg
    21781 tccgtgaaga ggtggcggca aatcttgaag tcttgctgtt attgatgttt atggtggcgg
    21841 gtatctattt tatgaaacag ctgttgctgt tcatatttac ccgtttgctg ttaagcattc
    21901 gctccaaaat gctgctgtcg ctctcttttt gcgtggcggc agcgttcctc tccgcgttcc
    21961 tcgatgcctt aaccgtcgtg gcggtggtga tcagcgttgc tgtcggtttt tatggtattt
    22021 atcaccgcgt agcctcttcc cgtaccgaag ataccgacct gcaagacgat agtcatatcg
    22081 acaagcatta caaagtggtt ctggaacagt tccgtggctt tctgcgtagc ctgatgatgc
    22141 atgccggtgt cggcactgca ttaggcggcg tgatgaccat ggtgggcgaa ccacagaacc
    22201 tgatcatcgc taaagcggct ggctggcatt ttggcgattt cttcctgcgc atgtcgccgg
    22261 tgaccgttcc ggttctgatt tgtggcctgt taacctgcct gctggtagag aagctgcgtt
    22321 ggtttggcta cggtgaaacg ctgccggaga aagtccgcga agtgttgcaa cagtttgacg
    22381 atcaaagccg ccaccagcgt acccgtcagg ataaaatccg tctgattgtc caggcgatta
    22441 ttggcgtctg gctggtgact gcgctggcgt tgcatctggc ggaagttggc ttgattggtt
    22501 tgtcagtcat tattctggcg acatcattga ccggtgtcac cgatgaacat gctatcggta
    22561 aagccttcac cgaatctctg ccattcaccg cactgttgac ggtgtttttc tcggtagtcg
    22621 cggtgattat cgaccaacaa ctgttttcgc caattattca gtttgtgttg caggcatcgg
    22681 aacatgctca gctgtcgctg ttctatattt tcaacggtct gctgtcatcc atttcggata
    22741 acgtcttcgt ggggacgatt tatatcaacg aagcgaaagc ggcaatggaa agtggcgcta
    22801 tcacgttgaa gcaatacgag ctgctggcgg tcgccattaa taccggtacc aatctgccct
    22861 ccgtcgctac gccgaacggt caggctgcgt tcctgttcct gctgacctct gcactcgcgc
    22921 cattgattcg cctctcttat ggccgcatgg tgtggatggc cctgccttac accctcgtac
    22981 tgacactcgt cggcttgctc tgcgtcgagt ttacgcttgc ccctgtaacc gaatggttta
    23041 tgcaaatggg ctggatagca acgctttgat aacaacttac cgggcatata aatgcccggt
    23101 ttgccttttc gcccgataat tgtccaattg cttcctattt ttgctgcctc ctggtggcgc
    23161 agcgaatgaa ttggtttaaa ctgcgcactc tatgcatatt gcagggaaat gattatgttg
    23221 cgatttttga accaatgttc acaaggccgg ggcgcgtggc tgttgatggc gtttactgct
    23281 ctggcactgg aactgacggc gctgtggttc cagcatgtga tgttactgaa accttgcgtg
    23341 ctctgtattt atgaacgttg tgcgttattc ggcgttctgg gtgctgcgct gattggcgcg
    23401 atcgccccga aaactccgct gcgttatgta gcgatggtta tctggttgta tagtgcgttc
    23461 cgcggtgtgc agttaactta cgagcacacc atgcttcagc tctatccttc gccgtttgcc
    23521 acctgtgatt ttatggttcg tttcccggaa tggctgccgc tggataaatg ggtgccgcaa
    23581 gtgtttgtcg cctctggcga ttgtgccgag cgtcagtggg attttttagg tctggaaatg
    23641 ccgcagtggc tgctcggtat ttttatcgct tacctgattg tcgcggtgct ggtggtgatt
    23701 tcccagccgt ttaaagcgaa aaaacgtgac ctgttcggtc gctaatccct tcagcgctcc
    23761 tgcgggagcg cttttttcct gccgctatat ttatttgacc cgcagtaaat cagaacttcg
    23821 cgttgtataa cgtggtgaaa gcatggcacg cttcatctgc cattgttgct ggatcccctg
    23881 cccggcaaaa tagagtgttc ctctgccctc tttagcattc agtgtatcca ttaccgtcat
    23941 caattgctca ctcccggggc gcggtgcgtt gtcatcgaat aaattgagct gcgcgactcc
    24001 ctgactgaag aaatccccca gcatcacgcc cgctttttgg tagcgatggc ccgcttgcca
    24061 gatggcatcc agagatcgcg tagcagcgtt aatgatatcc ctgctgtcct gagtgggcgt
    24121 cagcagtttt accgacgcgc tattgccgta ataaggttca ttgagcgcaa atggtgacgt
    24181 cttaataaat gtggagataa accgacaata ttgatgctcg ctgcgaagtt tttccgccgc
    24241 ccgggcagcg taactacaaa tggcctgccg catcgatgga taatccgtga tgcgttcacc
    24301 aaacgagcgg gaacagataa tttcctgctt cgtcggtgca aactcttcca gttgcaaaca
    24361 gggttcgccg cgcagttcac gcaccgttct ttcgagcacg acattaaaat gtttacggat
    24421 aaaccggata tctgtatccg ccaaatcgag aacggttttg atccccatcg cgtccagttt
    24481 tttgctgatc cgccgtccaa tcccccagac gtcatccacg gggagagcag acattaattt
    24541 acgctggcgt tccagatttg ataaatccac caccccaccc gtctgccgct gccatttttt
    24601 tgccgcatga ttggcaagct tagccaacgt tttggtctgg gcgatcccca caccaacagt
    24661 aagatgggta cgttgtagca ccgttgcgcg aatttctctg ccaaaatcag tcagatcgcg
    24721 acaattacgc acacctgtca gatcgcagaa tgcctcatca atactgtaaa tctcgacgcg
    24781 gggcgatagt tcttccagcg tcgacatcac ccgattgctc atgtctgcgt aaagctcata
    24841 attgctgcta aagcaaacca cgccacagcg acgaaacaga tctttttgtt tgaaccaggg
    24901 atcgcccatt ttaacaccca gcgcctttgc ctcagcgttt cgggcgataa cgcaaccgtc
    24961 attattcgat agcacaacca ccggtttacc ccataaatca gggcgaaaca ccgtctcaca
    25021 gctggcataa aacgcgttta catcacagag agcaaacatc agcgcatcgc cttaacgacg
    25081 tggatcacca caccaaagac atccagcgta tcttcgctac tgatggtaat gggcgagtac
    25141 gcactgttca tgggaataag ctgtaccgtc gggcgtagtt gcaatttttt caccgtaaac
    25201 tcgccgtcaa cagcagcgat gacaatatca ccatggctgg cggtaatagc gctatcgaca
    25261 atcagtaaat caccgtcact aattccacca tcaatcatag aatcaccact tgctttgacg
    25321 aagtaagtcg cgctgggatg ctggatcaac agttgattca gatcgatgcg ctgttcaacg
    25381 taatctgctg ccggtgaagg aaagccacac tgaacaagat cgctaaatag cggaaaagtc
    25441 acaatttcgc ggagatccgc aggcttgata aacaacataa taatctgcct gaagttatac
    25501 tgtttttata tacagtagtc tattcttgcc agcagatcaa tactgattca ggctatcaat
    25561 atttgtcgct gcatagactg ctgatttttc gttctcttat cctgtgctca cgtggccttc
    25621 tggcgacgac gctcatccag cagaaatgaa aaatatcacc cggcaaaaaa atagaataga
    25681 agcattcgcc ataatgacat taaacattgt ttgatattta tcatattaat agaaataaag
    25741 acattgacgc atcccgcccg gctaactatg aattagatga agtaaaattt attaatagtt
    25801 gtaaaacagg agtttcatta caatttatat atttaaagag gcgaatgatt atgactgaaa
    25861 tcgttgcaga taaaacggta gaagtagtta aaaacgcaat cgaaactgca gatggagcat
    25921 tagatcttta taataaatat ctcgatcagg tcatcccctg gcagaccttt gatgaaacca
    25981 taaaagagtt aagtcgcttt aaacaggagt attcacaggc agcctccgtt ttagtcggcg
    26041 atattaaaac cttacttatg gatagccagg ataagtattt tgaagcaacc caaacagtgt
    26101 atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta tatttttcta tttgatgagt
    26161 acaatgagaa gaaagcatcc gcccagaaag acattctcat taaggtactg gatgacggca
    26221 tcacgaagct gaatgaagcg caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg
    26281 cttccgggaa actgctggcg ttagatagcc agttaaccaa tgatttttca gaaaaaagca
    26341 gcttttccag tcacaggtag ataaaatcag gaaggaagcg tatgccggtg ccgcagccgg
    26401 tgtcgtcgcc ggtccatttg gattaatcat ttcctattct attgctgcgg gcgtagttga
    26461 agggaaactg attccagaat tgaagaacaa gttaaaatct gtgcagaatt tctttaccac
    26521 cctgtctaac acggttaaac aagcgaataa agatatcgat gccgccaaat tgaaattaac
    26581 caccgaaata gccgccatcg gggagataaa aacggaaact gaaacaacca gattctatgt
    26641 tgattatgat gatttaatgc tttctttgct aaaagaagcg gcaaaaaaaa tgattaacac
    26701 ctgtaatgag tatcagaaaa gacacggtaa aaagacactc tttgaggtac ctgaagtctg
    26761 ataagcgatt attctctcca tgtactcaag gtataaggtt tatcacattt aaaaacacag
    26821 acgtttttta aagattgaga gttacctgtt gataataagg tatcgggtaa ctctcattat
    26881 gacagagcgc tttcaaaaac agggctacga aatggcgagc atccagacgt atattaagaa
    26941 agcccccatc aacatctatt aaatttatca ctgcgcccag tcaggtttat ttaagatatg
    27001 atcctgccag tcaatcactt ctgattcttt cactgcgata tgacgcacag agatacgttc
    27061 accatgcatt gccgctttcg aaccagtaag tagcggatgc cacgcaggta aatctttacc
    27121 ttccgccagc aaacgataag cgcaggtcat tggcagccat tcgaatgttg gcagattttc
    27181 acgggttaat ttaatgcagt cgggttcaaa ctcgaaacga cgttcgtagt tccgacattg
    27241 acaagtttta atattgagct ggcgacaggc gacgttagtg aagtagattt cgtcggtgtc
    27301 ttcatccatc agtttatgca ggcaacactg accgcaacca tcacacaacg actcccattc
    27361 cgcatcgctc atttcgtcca gggttttact ttgccagaaa ggtacatcgc tcatcaggtg
    27421 ttccgccatt acgttaaaac gcaccttata accagtctgg cacagcgatg caagttttgc
    27481 cgccgctttc aggcggcaaa aagtattaca aaacgcgagt tgtcaaagaa tgcccatcga
    27541 aagtgactgt cagctcatca ccgctttgca acgggccgac gccatcaggc gtgcctgtca
    27601 acacaacgtc accggccttg agggtaaaaa acttgctcat ataagcgatc aacggaacga
    27661 ttttatggat catgtccgca gtcgtacctt gctggcgttg ttcgccgttt acgctcaggc
    27721 ccagcgttgt attttgtgga tcgccggtaa attccgccgc ggggataaac ccggaaagcg
    27781 gacaagagtt atcaaacgct ttagcctttt cccacggctg cccggctttc ttcatttttc
    27841 cctgaacatc acgcaatgtc agatcaagcg ccacgccgta accggcaatg gctttgcgga
    27901 catgctcttc cgttgcctga cgcagcgtcg cgccaatcaa caccgccagt tcgacttcat
    27961 gatgaactga accgaaatcg gatgggatcg ccaatggctg ccgcagatcg cacagtgccg
    28021 tttctggttt aataaacagc actggctctt cgggcactgc gctgcccatc tctttaatat
    28081 gtttggcata gttactgcca acacagacta ctttactcac cggataatcc agcagcgcac
    28141 cttgccagtt gtgatgttga tacataacgt tcccctcacc ttatcgtgga tatgaaaacc
    28201 cgcgccgaca aagataatgc caccgccgga tatcggttat ttgttagtgt cgtctgtttt
    28261 ctgccccatg acggaaagat gttgcttcag caaatcttcg ggtggtggcg gtaactgcaa
    28321 atagtaacct tgctcggtta atgccagttt aaccttttca atatcagcat tgaccagttt
    28381 cttgcgccca tccagcggca gaatcatcgc taactgaggc tgaccaaaac ctttcatcag
    28441 ttcctcagga acacgcgaaa aatcgtcttt tttttcgaca tataaatagg tctggtcacg
    28501 cttgctgctt cgataaatca cacaaaacat acttttactc tttaagatgc caggttttgg
    28561 cataagctaa catcaatgaa acccgcgcca caagccagcc agctgtagat ttctgcggtt
    28621 tgttgagtcc atgccccttt ataagtaatc cccccggcaa aaaatgccgc gccgggattt
    28681 taccgaacgc agaaaagcca ctggaattct cccgtaaggg attgttatat tataacagtt
    28741 catcgtactc attctgaaca ggagactacc aatgaaaatc aagagcatca gtaaggctgt
    28801 attgctgctt gcactgctaa cctccacgag ctttgctgcg ggtaagaatg tcaatgttga
    28861 gttcagcaaa ggccatagca gcgctcagta ctccggcgaa ataaagggat acgattacga
    28921 tacatatacc ttctatgcca aaaaaggtca gaaagtgcat gtgagtatct ctaatgaagg
    28981 cgctgatact tacctcttcg ggccaggaat cgatgattca gttgatttat ccagatattc
    29041 accagaactc gatagtcacg gacaatactc actccctgct tctggcaaat atgagctgcg
    29101 agtgctgcaa acccgaaacg acgcccgtaa aaacaaaact aaaaaatata acgtcgatat
    29161 tcaaattaag taaatgtcaa ccgacgggga ctctccccgt cataaacgtt aacgccccac
    29221 tcctctctgt cgctcaagag gttaatgacg aatagcagac aatgccgaat agaacataat
    29281 gtaaacaagg catcactcac gacacataaa taacatcttt gtggtgattt gattgatttt
    29341 tccactccgc ttaccatcca caccaaaata acgatcatca cgacataatt tttctttaac
    29401 atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga tcgtagcctt gtaatgattg
    29461 taagttttca gcggcttttt cgccgagata tttccttgtt aactctgggg aaatgccatc
    29521 tgagttcaca caaatatacg ggtcgcaaac aacgccgcac gccggagatt gaagtccgcc
    29581 agcgtcatgt ccagacgcca ttgatgagaa catcatcccc acacctgaca atccgatata
    29641 aaaaatacgc atcatattca tagcacatcc tgataatcca accgtacaac acttatgctc
    29701 atttcatcat caatccgtaa tcaaaaccgc tgacctggac gagttacgat gacctgacag
    29761 aagtatcaga cgtcttctgc ggtttctatg atagagcaat tctgtggcgg atattaagat
    29821 aacgggtatc tgttctttaa tgatgccgca aaaaatggaa agttaagaaa gcctggcgtg
    29881 gtgaaaaaat acacttgcct caatactgga tatttaaaca ggtctggaga tgctgtcacg
    29941 attagggtgc ggaaaaagga agcgtaaata agaacaatca aaagtacgac ggcaatgggt
    30001 tgattgacaa gggtattttt taagctatga atcagcgcca tttatcacag aatagacttt
    30061 tactctgaat aaatgggagg gtgacttgcc tcaatataat ccagactata acatgcctta
    30121 tagtcttcgg aacatcatcg cgcgctggcg atgattaata gctaattgag taaggccagg
    30181 atgtcaaaca cgccaatcga gcttaaaggc agtagcttca ctttatctgt ggttcatctg
    30241 catgaggcag aacctaaggt tatccatcag gcgctggaag acaaaatcgc tcaggccccc
    30301 gcatttttaa aacatgcccc cgttgtactc aacgtcagtg cactggaaga cccggtaaac
    30361 tggtcagcga tgcataaggc ggtttcggca accggtttgc gggttattgg cgtaagtggc
    30421 tgcaaagatg cgcaacttaa agccgaaatt gaaaagatgg ggctgcctat cctgacggaa
    30481 ggaaaggaaa aagcgccgcg tccagctccc gcaccacagg ctccagcgca aaatacaacg
    30541 ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt ccggtcagcg tatttatgct
    30601 ccacaatgtg atctgattgt tacaagccac gttagcgctg gggccgaatt gattgccgat
    30661 gggaacattc atgtctatgg catgatgcgc ggtcgtgcgc ttgcaggggc aagtggtgac
    30721 cgggaaacgc aaatattttg tacgaacctg atggcggaac tggtgtccat cgcaggtgaa
    30781 tactggctga gtgatcaaat cccagcagaa ttttatggca aagcggcgcg actgcagtta
    30841 gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc tttttaacaa ggaatttcta
    30901 tggcacgcat tattgttgtt acttcgggca aagggggtgt tggtaagaca acctccagcg
    30961 cggccatcgc cactggtttg gcccagaagg gaaagaaaac tgtcgtgata gattttgata
    31021 tcggcctgcg taatctcgac ctgattatgg gttgtgaacg ccgggtcgtt tacgatttcg
    31081 tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt aattaaagat aagcgtactg
    31141 aaaatctcta tattcttccg gcatcgcaaa cacgcgataa agatgccctc acccgtgaag
    31201 gggtcgccaa agttcttgat gatctgaaag cgatggattt tgaatttatc gtttgtgact
    31261 ccccggcagg gattgaaacc ggtgcgttaa tggcactcta ttttgcagac gaagccatta
    31321 ttaccaccaa cccggaagtc tcctcagtac gcgactctga ccgtatttta ggcattctgg
    31381 cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat taaagagcac ctgctgttaa
    31441 cgcgctataa cccaggccgc gtaagcagag gtgacatgct gagcatggaa gatgtgctgg
    31501 agatcctgcg catcaaactc gtcggcgtga tcccagagga tcaatcagta ttgcgcgcct
    31561 ctaaccaggg tgaaccggtc attctcgaca ttaacgccga tgcgggtaaa gcctacgcag
    31621 ataccgtaga acgtctgttg ggagaagaac gtcctttccg cttcattgaa gaagagaaga
    31681 aaggcttcct caaacgcttg ttcggaggat aagttatggc attactcgat ttctttctct
    31741 cgcggaagaa aaacacagcc aacattgcaa aagaacggct gcagattatt gttgctgaac
    31801 gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt gcgtaaagat attcttgagg
    31861 tcatttgtaa atacgtacaa attgatcctg agatggtaac cgtacagctt gagcaaaaag
    31921 atggcgatat ttctattctt gagctgaacg tgaccttacc ggaagcagaa gagctgaaat
    31981 aagcccgctg taaaaacgca tttatcttca aggcagagtc atttctgcct tgagtttttc
    32041 atccctctca tccacgttgt ggtaaagcgg cgagtattct tgctgatact cctcattgct
    32101 atttccacct ccccctttac accttaaggc tgttcccacg cacagaacaa aatgtttatg
    32161 cctggttgag taaataaccc tgttgttagt gtggatatac tgggagatga taaccccgca
    32221 atttcattat tcattgttct cccataggat gaggtttccc gccgtaactg gcgggctttt
    32281 tttgccccag aattttctct ttcaacatcc tgtaaacgaa aactgcgccg aagcgcagtt
    32341 taatcgttga tggtcagaac gaccagttca caccagccac cgcgttccac ggggactcca
    32401 caccggcacc atggctatac cccaccccga aatgcccgct taacgtactg ctgaatgagg
    32461 ctttaatacc tgcctggtat attccacgtc tgcccgacaa atcattgaca aaattaccgt
    32521 cactattcac tttcacccga ttatcatcga caaattcttt gcgcacagcc gccttcaggc
    32581 agggttcaat ttccataccg ttccccagac gcatgttgta actcagcgtt gcacccagtt
    32641 cacgatatat actgcgggta tcgactgatt tcgattccat gccattggat aaatgatagt
    32701 agcgtctaag tgacctaagc tctgctgcaa catcagcccg caggcaccag accaggggat
    32761 tcatcctgaa gagacagcgc aggtgtattg tgttcaccgc tcaccaaaga catcatgatg
    32821 aaatgatgat atcccgcata agaaagaggc attttttaca cgcagtgcgc tgaagtgtag
    32881 ttggataaaa aatcactctt ttcagataat actgtcgtac gcttatcttt cgacaatgag
    32941 gccgtcggca aaataaaatg actaatgtaa tcgttgctga tgaatatctt ctgctcacat
    33001 aaaaatcaca caataatatt gataaatcag attatcttac ctttacaaca ttgcgttaac
    33061 agtcaatgac acttcttgaa taaaaatatt gtcaatcaat gagttattcc tcatttaaga
    33121 ttattctcat tcacgtaaaa aacataattc ctatcatcac agccacgctc attttgaatt
    33181 atctgaataa tagcgagcgt tctttagtta agcagatctt aattgaagac gaaattattg
    33241 tgtgtgcaac ttacttaata ccggacatct aagttgaaga aaaaaatact taccttcggg
    33301 cttatctcag cattgttctg ctccacgcca gcgatggcgg atatgaatcg caccacgaaa
    33361 ggtgcattgc taggcgcagg cgttggttta cttactggca atggtgttaa tggcgtactg
    33421 aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag aaaaaggccg cgacggtaaa
    33481 aatgcgcgta aaggtgcaaa agtaggtgcc gccgttggtg ctgtcactgg cgttctgacc
    33541 ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg gcggtacggg tggtgcaatt
    33601 ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg ctttactggc gggcctgttt
    33661 gttttcgcac caatgacctg ggcgcaggac tacaatatta aaaacggttt gccgtcagaa
    33721 acctatatta cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc acaggttgca
    33781 gaaattgttg cggtaatggg taatgccagc gttgccagcc gtgatttaaa aattgagcaa
    33841 tctccggatc tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc taaagacccg
    33901 caaatgctgt tgatcacggc aattgatgac accatgcgtg ctatcggcaa aaaataatat
    33961 ttcatatgta ccgcgtcgat gaatacgacg cggtttttta gctaacggcg ggatcagaac
    34021 ttatatttca ggcccaccat cactgcggtg tcattgtagc cattatcacc cagctgcacg
    34081 cccacattcc cccagacgct ggccgctgga ttcaggttgc catttacgcc aagtttaatt
    34141 tcccccaaat tactcgcccc atcttgttcg actttcacac cgttcattga gacagcaaaa
    34201 tctttactgt tatggatcca gttcatttca acaaaaggtt caaagtcgtc accgtgcgga
    34261 ccgctgtttt tctcctgagt acgaataaag gtgcggaaac caagtcgggt ctggatgttg
    34321 ttatttgcgt cattctcaat acgtgagcca ttggcttcgg tgtgcgcatt atgatcgacg
    34381 cccatccaga tagcctgtgc ctgtggctgg aatatccacg tgtatttcgc cgcttccaga
    34441 ccaaaggatt tattcagggc aaagccgtaa cccgcttcca gcgagacggt tgcgcctttc
    34501 gcactatagg attcataggc taattcatcc cctttcaccg agtttttaaa ccagctatat
    34561 tgcgcccagg cgtcaatgta tgcgcctttt ttactgatgt catcggcaaa ccaggtggca
    34621 tacagccctg cgcaatagcc tctgacactc cctttcgaac gataatccga cacgctggaa
    34681 tgagttgagt tgtagtcgcg ggcataacca gccatcactc ccaaacgcca actgtcactg
    34741 tcggtaaaac caccggttaa cagatcggcc cctaactgcg agacgtagcg atgcgaggtc
    34801 gttctcaact gtccgttgct gtcacgccag gcattatgcc cgccaatttg acgtagccaa
    34861 agtcggcttg aacgctcctg ttcagttaca ggatcgatgt agcgcatttc acccgcacgg
    34921 tcgttcagac gcataacaaa aagagtgtta gcggctgcca ggttggcgac gtaacttcca
    34981 gcttcgaggc gcaggacggg ggtaggcgca acagtaggcg ctgaacttcc tccggagttt
    35041 gtttcaccgc cgctcgttgc ctggaagtta gtcagatacc agtttttatt ggtcgcttcg
    35101 gtattacctc gggttaatga atattcgtag gcaccggcga aaatcctcga atccttaata
    35161 aattccccat ttgattcccc ctcaacgctg ataatctcta tcccatttaa cgtgtaagct
    35221 ccgctgcctc ctgcattggt aactttaact cgggttgttc cagaggtatt tccattaata
    35281 acgactttat ccgtagctga attatcatca cccaattcag ttctgagatg caatacgcca
    35341 ttattaccaa cgtaattttc agttattgtt aatcttgttg gttcgacatc ccgtccatct
    35401 gcccgggaaa tatagaccgt actattatca accgtgaggt tgctcaccgt tgagtcagct
    35461 ttaatatccc aggtactatt atctgaaagc gacagatagg tatgagtgtt atcatctgtt
    35521 gaaatattag ctgaacccgt tatttttgaa ttagttgcat cgacggtcaa atgaccttta
    35581 atattattga taaaaacatc tgcagccgat gatacctcac tatccgtaat ggtcacgtta
    35641 ctcgtctcat ttttatggta gaggatttca tttgctgacc gtagtgagct accagataaa
    35701 atatctaccg ttgcttcctg accatttatg gaagactcca gcacaattcc agacccctga
    35761 tgcgcgactg cagatgtatt tttcagcgtc accgtgccgc cattataaat tttagcagtc
    35821 gcggcgtcag gacccgttgt ataaatttct ccctggttaa atgaaactgt cccctggcgg
    35881 gcagcgatgg catgagcatt gtccccatgc gtggtgatat caacatcggt taatgtaacg
    35941 ctggcattgg tataatcaag cgtgtcacca gccttgacga cagtgccatt agcgtacgcg
    36001 gcataacttt ctgttccgtg ggttgtgatc tccccttttg ccatatccag ttttgctatg
    36061 gcattatttt cattacttcc tgtatcggta gtactttctt catcaagaga agatacacac
    36121 gcacgcgcgc ttccgggaga tgcacacgca agaacgccga ctgcgtgatt acccgcagta
    36181 acaataaatg tatcatctcc gtcagaaaaa gagggggtaa ccgaaccgtc agagttgact
    36241 cttattatgg tgtcagtatt caggtttcca ctcgtttgac ttagcaaacc aattgagtaa
    36301 tcccccgtta tgacaatcgt gtttttaccc tgcaaaataa cagcaccgcc aaattcactt
    36361 acgattgcgc tagctgttaa tgctgtgctt agaggactat tgtcagaagg atataaaccg
    36421 cccgttgtta caatgacgcc attgttaatg gttaacttgc taccctctcc tgcaacggat
    36481 acccgtaact tccattaccc gttgttataa ctagcgcatt atcaatatct tttacttttc
    36541 catcttttat tgccacagca tgtttatatt catcgtgctg aataaactgt tcagcatatg
    36601 cattacccac cgccagagca ataagtccca aactaaaccc ggagagtttt ttcagtttca
    36661 tagattaacc atccattcat tggattaaca aacattcctg gtcaaataac ctatgcgttc
    36721 agattaccaa aacggagggt aaagacaaaa acaaaagcac aaaaaattta aatagataaa
    36781 cagcgacaat atataaatga taaataagtc ggcattatca tttttcacct tattttcatg
    36841 acattgatca ctttagggtg acgattttgt ttaacaaata actcttcgac atcaaaattg
    36901 ttaatagttg cgatgaggat tatttcggtg atatataaaa acaaccgtga taacaccagg
    36961 gttgacaact atttgcgcaa taatgccttt ccttcaatga tccaggcgtt cactgccagc
    37021 aacccattca gccgcatact gacagtgaga aactgaaaga ttattgctga tccctgtggt
    37081 tgacgagtct ttctcttaat cgattaacca gattgatcgc cagccaaaca agggcattaa
    37141 gcaaatttat cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga
    37201 ccaggagggt gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca
    37261 gattattact attgttcatg ctactaatgg caatcctgtc aaaactttca aacaattata
    37321 gcaaacaaaa taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt
    37381 acaccgcact atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt
    37441 agaaaggata attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg
    37501 ctgaaatgtg atattatttt ggttcagata gtcaagttgt tctttcgttt cgacaccttc
    37561 agcgacgata ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt
    37621 tgattctggg tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat
    37681 atgcaggtca tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat
    37741 gacaaaacca ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa
    37801 tggctcacgc tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg
    37861 gaaatttaaa cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat
    37921 tccaatatga aaaccttccg gcaatttact cgcgatagcg ttcatctgtc tggcaacctg
    37981 attcatcagg ctttgcgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa
    38041 tgccgcgggt gatatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc
    38101 ccgcaatgtc ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt
    38161 atttattgcc cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag
    38221 caggaaggca gcggcgcagg cgattaacag aataaatatt aaaattccaa agcctttatc
    38281 gataagtctg tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt
    38341 tgtggagtga accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt
    38401 tggtccaata atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt
    38461 aagcgccccg cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg
    38521 gttctgatag agcaggatag gtaatctatt aacgatgtcg attgctggag ccaacagtaa
    38581 attactgtcc gggaaaacag gaatacgaga caacaggacc cgattcccag gcagggatgt
    38641 acaccagact attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc
    38701 ttcagtgcca agctgatatt gctcctcgag gttgcattcc ttcccggcaa tgttaaccgc
    38761 tgtttgagtc gcttgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct
    38821 cgctcccgcc aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac
    38881 accggtaata aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt
    38941 gtcccggcca aagggtttaa tacatgaaaa tgccattcat tcgctcgcgc tggcatctac
    39001 agagagcatg gtagagagca taagaaatat ccacgccgaa gataaaattt tctccatctt
    39061 gatctaaccc attaattatt gagcataatg taggcagtac aaaataagtt aggcgggata
    39121 tcaggcgtca agaatggaga gcgagaactc tccattcttg acacctgata ttgcggacat
    39181 aataagaaag cataacgcct gaaatgctca ctttgcatca gcatggtgat acagctgatg
    39241 tttattctaa aaccttactc aagttctaag agagcacgga ttccctgtca ttaataatga
    39301 tatccgttag tgtagtctta tgcatttcaa tgtactgcaa tatttgcaga tagagatcag
    39361 aaagaatcac actggtttca tcttttaaac gcaagcgtaa agcctgatca ataatatgac
    39421 tatcaacata cccatgcgtc atgaacacct ccctctcgag atttttaaac aaagcacatt
    39481 gaaaaataaa tctctctggg attgaattat tcatgtcagc ctcgtagaaa tattaaccca
    39541 ataaaattat gctgtgtata gtctaaagcg acagacaaaa ataagatgaa tgagttttct
    39601 ataaacttat acttaataat tagaagttac atatcatcag ctgtgtatcg caacacgatt
    39661 tccagtgttt ttctaatcac atcagctttg agaatatcac ttgttgtttc cagggagtgt
    39721 atcagactta agataatatt tttattatta acattatcgc ctgaaagcat taaattggtg
    39781 acagcctgcc ctaacactgc tgactcttct tctaacaaat tacccgaact gcgaaagtaa
    39841 cttgctaacg ctttatcagt tattgcatta tgaattgtag tatcttcaag catggtaacc
    39901 tctcatctta cttatgaaat tttaatgtat tctgtttatt ttcttaccat tgaacatact
    39961 tttattgtca gctgtcgctg ttcttcggtt tggtcctcct gaatcactat tgctaaaatc
    40021 acgaattatg ctgtcaatta aatcgcgtcg gaaagcatct tcctcagccg atttcattac
    40081 gagtaattgc ttaataagag tctgcaatga aaaatttatc ttcatgcgca gtatctggat
    40141 caccgcctgg ccagtaattt catattttat acgttcatta tcagatgtct tcataacgac
    40201 ttcatagcta acctgttaaa taatcaggct gttgcattat tcaaaaagca acccaattag
    40261 tgcattataa tgtttctgtt cctcttcccc agtcgcgtgt tccagtcggc aaagaagttt
    40321 ggcacaaagc gactttcgac tcagattacg tccatctttc aaaatctcag tcacaatttc
    40381 cccgagagtt tcctgctgag taggcaagtt agcttttgca aagtaacgag tgattgctcc
    40441 cgcagaatct aaagtcactg aattttgatg catgctacgc ctctgttaaa aatgttaatc
    40501 aatgttaagt gaggtaacaa tagcaaactt gtacgaaata tgtgtacaga atgataaatt
    40561 aattttttgc aattagtgct aatgcatata aaacaatgtg ttatgataaa acatattgca
    40621 tcatttacct gacaatctat acaagatttt tcgccaacta gtgttcaact ttttccattt
    40681 tttatcaatg taaagaaacg gttatgtttt ggtattaaaa gaaagacaca agaagatatg
    40741 tctgttaccg tcttactctc gcctcaccca ttaccctgga ttgttaatgc ttaccaccct
    40801 tatttatcgt agccatatac gtgacgacga acctgtcaaa aaaatcgaag aaatggtttc
    40861 gatagcaaat cgcaggaaca tgcagtctga cgtaacaggg atcttactgt ttaatggttc
    40921 tcattttttc cagcttctgg aaggtccgga agaacaggtt aaaatgatat atcgggctat
    40981 atgccaggat ccacggcact ataatattgt tgagctgctg tgcgattacg cgcctgctcg
    41041 ccgttttggc aaagcgggaa tggaattatt tgatttgcgc ctgcacgagc gagatgacgt
    41101 tttacaggcc gtattcgaca aaggcacatc aaaatttcag ctaacttatg atgacagagc
    41161 gctacaattt tttcgtactt ttgtccttgc aaccgaacaa tcaacctatt tcgagatccc
    41221 tgccgaagac tcctggcttt ttatcgctga cggatctgat aaagaacttg attcctgtgc
    41281 cctttcacca actataaacg accactttgc ctttcatcct attgtcgatc ccttatcgcg
    41341 gcggataatc gcttttgaag ccattgtgca aaaaaatgaa gatagcccat cagccatagc
    41401 ggttgggcag cgtaaagacg gggaaatcta cacagcggat ctcaaaagta aggcgcttgc
    41461 attcacgatg gcacacgcac ttgagctcgg tgataaaatg atttcaatca atctattacc
    41521 tatgaccctg gttaacgaac ctgacgcagt ctctttttta cttaatgaaa taaaggccaa
    41581 tgctctggtg cctgaacaaa tcatcgttga atttactgaa agtgaagtca tatctcggtt
    41641 tgatgagttt gccgaagcga ttaaatcgct aaaggctgct ggtatcagtg tagcaattga
    41701 tcattttggc gcaggttttg ctggtttgtt actcctgtca cgcttccagc ctgacagaat
    41761 taaaatcagt caggaattga ttaccaatgt tcataaaagt gggccacggc aggcaattat
    41821 tcaggcgatc ataaaatgct gtacatcact tgaaattcaa gtcagtgcta tgggcgtggc
    41881 aacaccagaa gagtggatgt ggcttgaatc cgcaggaatt gagatgtttc agggagatct
    41941 gtttgctaaa gctaaattga atggtatccc ttccatagcg tggccagaga aaaaataatt
    42001 ttcagcacat tctttcacat gatttcagta aatcatcgtg aaaatgaata cgttacgttt
    42061 aaatttaatc cacattaaaa ataatgtttc atccaattta gctga
//
LOCUS       DAZFYC010000030        41892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFYC010000030 DAZFYC010000000
VERSION     DAZFYC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.030"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            270..941
                     /locus_tag="ACHKW5_001816"
     CDS             270..941
                     /locus_tag="ACHKW5_001816"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO7511223.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARFAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQFFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            951..2147
                     /gene="yaiP"
                     /locus_tag="ACHKW5_001817"
     CDS             951..2147
                     /gene="yaiP"
                     /locus_tag="ACHKW5_001817"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7511224.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2125..2706
                     /locus_tag="ACHKW5_001818"
     CDS             2125..2706
                     /locus_tag="ACHKW5_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7511225.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKTGFATEIKNAVIEAQATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2708..3481
                     /gene="yaiO"
                     /locus_tag="ACHKW5_001819"
     CDS             2708..3481
                     /gene="yaiO"
                     /locus_tag="ACHKW5_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO7511226.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQC
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3666..3941
                     /gene="frmR"
                     /locus_tag="ACHKW5_001820"
     CDS             3666..3941
                     /gene="frmR"
                     /locus_tag="ACHKW5_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO7511227.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3976..5085
                     /gene="frmA"
                     /locus_tag="ACHKW5_001821"
     CDS             3976..5085
                     /gene="frmA"
                     /locus_tag="ACHKW5_001821"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO7511228.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5178..6011
                     /gene="fghA"
                     /locus_tag="ACHKW5_001822"
     CDS             5178..6011
                     /gene="fghA"
                     /locus_tag="ACHKW5_001822"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7511229.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6136..6675)
                     /gene="yaiL"
                     /locus_tag="ACHKW5_001823"
     CDS             complement(6136..6675)
                     /gene="yaiL"
                     /locus_tag="ACHKW5_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO7511230.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6777..7988)
                     /gene="mhpT"
                     /locus_tag="ACHKW5_001824"
     CDS             complement(6777..7988)
                     /gene="mhpT"
                     /locus_tag="ACHKW5_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO7511231.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8263..9276)
                     /gene="mhpE"
                     /locus_tag="ACHKW5_001825"
     CDS             complement(8263..9276)
                     /gene="mhpE"
                     /locus_tag="ACHKW5_001825"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO7511232.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9273..10223)
                     /gene="mhpF"
                     /locus_tag="ACHKW5_001826"
     CDS             complement(9273..10223)
                     /gene="mhpF"
                     /locus_tag="ACHKW5_001826"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO7511233.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10220..11029)
                     /gene="mhpD"
                     /locus_tag="ACHKW5_001827"
     CDS             complement(10220..11029)
                     /gene="mhpD"
                     /locus_tag="ACHKW5_001827"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO7511234.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11039..11905)
                     /gene="mhpC"
                     /locus_tag="ACHKW5_001828"
     CDS             complement(11039..11905)
                     /gene="mhpC"
                     /locus_tag="ACHKW5_001828"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO7511235.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11923..12867)
                     /gene="mhpB"
                     /locus_tag="ACHKW5_001829"
     CDS             complement(11923..12867)
                     /gene="mhpB"
                     /locus_tag="ACHKW5_001829"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO7511236.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12869..14533)
                     /gene="mhpA"
                     /locus_tag="ACHKW5_001830"
     CDS             complement(12869..14533)
                     /gene="mhpA"
                     /locus_tag="ACHKW5_001830"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO7511237.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14724..15557
                     /gene="mhpR"
                     /locus_tag="ACHKW5_001831"
     CDS             14724..15557
                     /gene="mhpR"
                     /locus_tag="ACHKW5_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO7511238.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRPMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15634..16716
                     /gene="lacI"
                     /locus_tag="ACHKW5_001832"
     CDS             15634..16716
                     /gene="lacI"
                     /locus_tag="ACHKW5_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO7511239.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16839..19913
                     /gene="lacZ"
                     /locus_tag="ACHKW5_001833"
     CDS             16839..19913
                     /gene="lacZ"
                     /locus_tag="ACHKW5_001833"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO7511240.1"
                     /translation="MTMITDSLVVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWNKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAANLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFIRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGSHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19965..21218
                     /gene="lacY"
                     /locus_tag="ACHKW5_001834"
     CDS             19965..21218
                     /gene="lacY"
                     /locus_tag="ACHKW5_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO7511241.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21284..21895
                     /gene="lacA"
                     /locus_tag="ACHKW5_001835"
     CDS             21284..21895
                     /gene="lacA"
                     /locus_tag="ACHKW5_001835"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO7511242.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(21998..23179)
                     /gene="cynX"
                     /locus_tag="ACHKW5_001836"
     CDS             complement(21998..23179)
                     /gene="cynX"
                     /locus_tag="ACHKW5_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO7511243.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     SWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23185..23655)
                     /gene="cynS"
                     /locus_tag="ACHKW5_001837"
     CDS             complement(23185..23655)
                     /gene="cynS"
                     /locus_tag="ACHKW5_001837"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO7511244.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23686..24345)
                     /gene="cynT"
                     /locus_tag="ACHKW5_001838"
     CDS             complement(23686..24345)
                     /gene="cynT"
                     /locus_tag="ACHKW5_001838"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO7511245.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24455..25354
                     /gene="cynR"
                     /locus_tag="ACHKW5_001839"
     CDS             24455..25354
                     /gene="cynR"
                     /locus_tag="ACHKW5_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO7511246.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASWALHELGAGKRAIHDVAALTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25394..26677)
                     /gene="codA"
                     /locus_tag="ACHKW5_001840"
     CDS             complement(25394..26677)
                     /gene="codA"
                     /locus_tag="ACHKW5_001840"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO7511247.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26667..27926)
                     /gene="codB"
                     /locus_tag="ACHKW5_001841"
     CDS             complement(26667..27926)
                     /gene="codB"
                     /locus_tag="ACHKW5_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO7511248.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(28169..30055)
                     /gene="prpE"
                     /locus_tag="ACHKW5_001842"
     CDS             complement(28169..30055)
                     /gene="prpE"
                     /locus_tag="ACHKW5_001842"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO7511249.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(30095..31546)
                     /gene="prpD"
                     /locus_tag="ACHKW5_001843"
     CDS             complement(30095..31546)
                     /gene="prpD"
                     /locus_tag="ACHKW5_001843"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO7511250.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31580..32749)
                     /gene="prpC"
                     /locus_tag="ACHKW5_001844"
     CDS             complement(31580..32749)
                     /gene="prpC"
                     /locus_tag="ACHKW5_001844"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO7511251.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(32794..33684)
                     /gene="prpB"
                     /locus_tag="ACHKW5_001845"
     CDS             complement(32794..33684)
                     /gene="prpB"
                     /locus_tag="ACHKW5_001845"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO7511252.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33923..35509
                     /gene="prpR"
                     /locus_tag="ACHKW5_001846"
     CDS             33923..35509
                     /gene="prpR"
                     /locus_tag="ACHKW5_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO7511253.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35607..35882)
                     /gene="yahO"
                     /locus_tag="ACHKW5_001847"
     CDS             complement(35607..35882)
                     /gene="yahO"
                     /locus_tag="ACHKW5_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO7511254.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            36029..36700
                     /gene="yahN"
                     /locus_tag="ACHKW5_001848"
     CDS             36029..36700
                     /gene="yahN"
                     /locus_tag="ACHKW5_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO7511255.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36717..36962)
                     /gene="yahM"
                     /locus_tag="ACHKW5_001849"
     CDS             complement(36717..36962)
                     /gene="yahM"
                     /locus_tag="ACHKW5_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO7511256.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(37375..38190)
                     /gene="yahL"
                     /locus_tag="ACHKW5_001850"
     CDS             complement(37375..38190)
                     /gene="yahL"
                     /locus_tag="ACHKW5_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO7511257.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38433..39482)
                     /gene="yahK"
                     /locus_tag="ACHKW5_001851"
     CDS             complement(38433..39482)
                     /gene="yahK"
                     /locus_tag="ACHKW5_001851"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO7511258.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39569..40525)
                     /locus_tag="ACHKW5_001852"
     CDS             complement(39569..40525)
                     /locus_tag="ACHKW5_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7511259.1"
                     /translation="MKKSWRNNVEFYLIGLLALTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(40522..41493)
                     /locus_tag="ACHKW5_001853"
     CDS             complement(40522..41493)
                     /locus_tag="ACHKW5_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7511260.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(41486..>41892)
                     /locus_tag="ACHKW5_001854"
     CDS             complement(41486..>41892)
                     /locus_tag="ACHKW5_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7511261.1"
                     /translation="KDRFGFLSETKVRDLVDRLIKALTIKVSDPHLPVNTLSGGNAQR
                     VSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVLMICDEIEEAWYQ
                     SHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
BASE COUNT        10072 a        11329 c        10699 g         9792 t
ORIGIN      
        1 tattttttaa atacaattaa ttgacaaact aaatcacatc taaatacaat gtataaaaca
       61 aaataactat acataccaaa catacaaaca cttaacatat gaattgcata gaaacattac
      121 ccagtcgaac ctccgggtct gaaagccctt gcttttcagt tattagcaac atttaacatt
      181 tataaattaa catctggctg cgccagagat tacgtctctg agtgcatcgt tgtgcttgta
      241 gtataataaa taaaatggag tgtcattaag tggataaggt tttagattca gccctccttt
      301 cttcggcaaa taaaagaaag ggtatccttg ctattggcgc acatcctgac gatatagaat
      361 taggctgtgg cgcatcgctt gctcgttttg cgcaaaaagg aatttatatc gcagccgtgg
      421 tgatgactac cggcaactct ggcacagatg gaataatcga tcgccatgaa gaatcgcgca
      481 acgccctaaa gatattaggg tgccaccaaa ctattcatct taattttgct gacacccgcg
      541 ctcatttaca gctcaatgat atgatttccg ccctggaaga catcattaaa aatcaaattc
      601 cttctgatgt tgaaatcatg cgggtatata ccatgcatga tgccgaccgc catcaggatc
      661 atctcgctgt ttatcaagct tcaatggttg cctgccgcac tattccacaa attctcggct
      721 acgaaacccc aagtacctgg ctttcattta tgcctcagtt ttttgaatcc gttaaagaag
      781 aatatttcac agtcaaactt gctgcattaa aaaaacataa aagccaggaa cgacgcgatt
      841 atatgcgtca tgatcgcctg cgtgcagttg cacaatttcg cgggcaacag gtcaatagcg
      901 atctgggtga aggctttgta attcataaaa tgattctttg agcaaattgc atgaaaacct
      961 ggatatttat ctgtatgtcc atagcaatgt tgctatggtt tttaagtacg ctaagacgta
     1021 aacccagtca aaagaaaggc tgtattgacg ccattatacc tgcgtataac gaaggcccgt
     1081 gtctggcgca gtcactggat aatctactgc gtaaccctta tttttgccgg gtaatttgcg
     1141 ttaacgacgg ctccacggac aataccgaag cggtcatggc ggaagtcaaa cgcaaatggg
     1201 gcgaccgctt tgttgccgtc acgcaaaaaa ataccggtaa aggtggtgcg ctgatgaatg
     1261 gcctcaatta tgccacctgc gaccaggttt ttttaagtga tgccgacacc tatgttccgc
     1321 ccgatcaaga cggaatgggc tatatgctgg cagaaattga gcgcggtgct gatgccgtag
     1381 gcggcattcc ctctactgcg ttgaaaggcg cggggctgtt accgcacatc cgcgcgaccg
     1441 taaagttgcc gatgattgtt atgaagcgca cgctacagca gctcctgggc ggcgcaccgt
     1501 ttattatcag cggtgcctgc ggaatgttcc gcactgatgt attgcgtaag ttcggtttct
     1561 cagatcgtac taaagtcgaa gaccttgatc tcacctggac attagtggca aacggctacc
     1621 gtattcggca ggcgaatcgc tgcatcgtat acccacagga atgcaacagc ccgcgtgagg
     1681 agtggcgtcg ctggcggcgt tggattgtgg gatacgcggt ctgtatgcgc ctgcataaaa
     1741 gacttttatt tagccgcttc ggtatcttca gtatatttcc tatgctgttg gttgtgattt
     1801 atggcgttgg gatttatctc actacctggt ttaatgaatt catcaccacc gggccgcatg
     1861 gtgtggtgtt ggcaatgttt ccgcttatct ggatcggcgt agtttgtgtt attggtgctt
     1921 ttagcgcctg gtttcatcgt tgctggttgt tggtgccttt agcgccgctt tccgttgtgt
     1981 atgtattatt agcttatgcc atctggatta tttatggact tattgccttt tttactggac
     2041 gcgaacctca gcgcgacaaa cccacccgct attccgcact ggtggaagcg tcaaccgctt
     2101 attcccaacc ttctgtcaca ggaactgaaa aactatctga agcttaatgt taaagagaaa
     2161 aatattcaga ttgcagacca ggtaattatt gatgaaactg caggtgaagt tgttatcggc
     2221 gcgaataccc gtatttgtca tggtgccgtt attcagggtc cggtagtgat tggcgcaaac
     2281 tgcctgatag gtaattatgc gtttattcgt cctggcacaa taatcagcaa tggtgtaaaa
     2341 actggttttg ccaccgaaat taaaaatgcg gttattgaag cgcaagcaac gattggtccg
     2401 caatgtttta ttgccgactc ggtagttgca aaccaggcat atttgggcgc acaagtacgt
     2461 accagtaatc atcgtctgga tgaacagcct gtgtctgttc gaactccaga gggaattatc
     2521 gctaccggat gcgataaatt aggttgttat atcgggcagc gttcacgcct tggcgtacaa
     2581 gttataattt tgcctgggcg aattatttcc ccgaacacgc aacttggccc gcgcgtgatt
     2641 gtagaacgta atttacctgc cggaacttat tcactccgac aagaacttat ccgtacagga
     2701 gattaaaatg ataaaacgga cgttattagc ggcggccatt tttagcgcat tgcccgctta
     2761 tgccgggtta acttccatta ccgcgggcta cgattttacc gattattctg gcgatcatgg
     2821 caaccgtaat ttagcgtatg ctgaactggt ggcgaaagtt gaaaacgcaa cgctgctttt
     2881 taatctttcc caggggcgtc gtgattatga aactgaacat ttcaatgcca ctcgcggtca
     2941 gggtgcggtc tggtataaat ggaataactg gctgacaacc cgaacgggta ttgcctttgc
     3001 ggataatacg ccggtctttg cccgccagga ttttcgtcag gatattaacc tggccctgtt
     3061 gccaaaaacg cttttcacga ccggttatcg ctacactaaa tattacgatg atgtcgaagt
     3121 cgatgcctgg caaggcggcg tatcactcta tactggcccg gtgatcacca gctaccgcta
     3181 tacccattat gactccagcg atgcaggtgg tagttatagc aatatgattt ccgtgcgtct
     3241 gaatgacccg cgcggcactg gttatacgca actatggcta agccgcggaa caggcgctta
     3301 cacctatgac tggacgccag aaacacgcta cggcagcatg aagagcgtca gtttgcaatg
     3361 tattcaaccg ctgactgagc aacttaatct cgggctgacg gcaggtaaag tgtggtacga
     3421 caccccaacc gatgattata acggtctgca acttgcagcc catctgacct ggaaattctg
     3481 attccttctg ccgcccgcta tccggggcgg ccttccctgc cgattagccc cccctttcct
     3541 ctttgttttc cgaccacatt caccggataa attttattct ccagtgttat atactatagg
     3601 ggggtatgca ttgacatata gcataccccc ctatagtata ttgcgtgcag atgatgaggt
     3661 gcgaaatgcc cagtactccg gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc
     3721 gagggcagat tgatgctctg gaacggtcgc tggagggtga tgccgaatgc cgtgccatac
     3781 tccaacagat cgctgccgtt cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa
     3841 gccatatccg ggaaacgttt gaccgaaatg actgctacag ccgcgaagtc agccaatccg
     3901 ttgacgacac tattgaactg gttcgagcct atcttaaata gctgaatcta ttaccatatt
     3961 gaggaagagc gagagatgaa atcacgtgct gccgttgcat ttgctcccgg taaaccgctg
     4021 gaaatcgttg aaattgacgt tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc
     4081 cataccggcg tttgccatac cgacgcattt accctctccg gggatgaccc ggaaggtgta
     4141 ttcccggtgg ttctcggtca cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta
     4201 accagcgtca aacctggcga ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt
     4261 gagttctgtc gttctggcaa aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa
     4321 ggcttgatgc cagacggcac cacccgtttt tcttacaacg ggcagccgct ttatcactac
     4381 atggggtgct ctacattcag tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt
     4441 aatccagaag caaaccatga acacgtctgt ctgctgggct gtggcgtgac caccggtatt
     4501 ggcgcggtgc acaacacagc taaagtccag ccaggtgatt ctgttgccgt gtttggtctt
     4561 ggcgcgattg gtctggcagt ggttcagggc gcgcgtcagg cgaaagcggg acggattatc
     4621 gctatcgata ccaacccgaa gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc
     4681 attaacccga atgactacga caaaccgatt aaagatgtcc tgctggatat caacaaatgg
     4741 ggtatcgacc atacctttga atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa
     4801 agtgcgcacc gcggctgggg tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa
     4861 atctccaccc gtccattcca gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc
     4921 ggcgtgaaag gtcgttccca gttaccgggt atggttgaag atgcgatgaa aggtgatatc
     4981 gatctggaac cgtttgtcac gcataccatg agccttgatg aaattaatga cgccttcgac
     5041 ctgatgcatg aaggcaaatc cattcgaacc gtaattcgtt actgatttcc cgcaggtata
     5101 ccccgtccac ttcagacggg gttcttaata ctctccccgg gcagccgtcc gggggattaa
     5161 ccgtgagata atgactgatg gaactcattg aaaaacatgc cagctttggc ggctggcaaa
     5221 atgtgtatcg gcattattcc caatcactga aatgtgaaat gaatgtcggc gtctatctcc
     5281 caccaaaagc cgcgaatgaa aaattgccgg tgctgtactg gctttcgggc ctgacctgca
     5341 acgagcagaa tttcattact aaatcgggga tgcagcgtta cgcggctgaa cacaacatta
     5401 ttgttgttgc gccggacacc agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg
     5461 atctcgggca aggtgccggg ttttacctga acgcaacgca agcgccgtgg aatgaacatt
     5521 acaaaatgta tgactatatc cgcaacgagc tgccggattt agtgatgcaa cattttccgg
     5581 caacgaccag aaagtctatc tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg
     5641 cgttacgtaa cccagatgaa tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat
     5701 cgcaagtacc gtggggacag caagcctttg ccgcatatct tggtgaaaat aaagatgcct
     5761 ggttgaatta cgatccggtg agtcttattt cacaaggtca acgcgttgcg gaaatcatgg
     5821 ttgatcaggg gttgagtgat gatttttacg cggaacagct gcggactcca aatcttgaaa
     5881 agatctgcca ggagatgaat atcaagacgt taatccgtta tcacgagggt tacgaccaca
     5941 gctattattt tgtctccagt tttattggcg agcatattgc ctaccacgcc aataaactga
     6001 atatgcgttg ataatagtgc acgactgccg gatgcggcgt gaacgcctta tccgcctaca
     6061 tttcgcccgc aggccgtagg catgataaga cgcgcatagc gtcgcatcag gcatctgcgc
     6121 acgacttatc tatgtttacc acatcaaatc atcaggcact ttgaagtcgg catatgggtc
     6181 gtcttcatcc tgctcttcgg cactgagcgc gctgtgcaac acaatactgc tggcatcacg
     6241 ctgagcaatt ttatcggcaa cgctggcggg aataatggcg tattcacctt cgctattgtt
     6301 atcgaccaac aagcgggcaa tcgccagacg accattaatc aactgcgcct gtgtgagctt
     6361 atcgacaaaa atcttcttaa tcagattgcc gtcagtgaag ttaaaaccaa tatcaccatt
     6421 ggcaatggtg attcggttca tttcaataag ctgcttcacc tgggctttat attctttcgc
     6481 caacgccgct tgtttttgct gttcgctaag ctgtttatca cgctcaagct gtgccttttt
     6541 attttcttct accgccgccc gagcttcacg cgcctgaacg cgcgattttt tcgccgttct
     6601 ctcgaccttc gccgcttttt tgctggtgac taatcctgct ttgagcaact gctcttgtaa
     6661 ggtaagtttt gccatctttg tttccaaacc cgctgaataa tttttagcga gtatacctgt
     6721 aaacgccgtg ggtgtattgg gggcaagtcc ctttcgcccc tgctaaataa ggcgcgtcag
     6781 gcatcggcac acggctgcat tcttgatctc cgactcatca aaataaacac cgccaaccca
     6841 gcaacaagaa tacccggtgc agaagcggcc attacgccga ccgtgccagt gcctaatgcc
     6901 agcattttcc cggccagtaa cggaccgctc atagccccca gacgccctac cgccacggct
     6961 gttcccacac ctgttgcgcg gatctgcgaa ctgtaaaaca acggtgccag ggcatacaaa
     7021 acgctttgcc cacctgtcgc aaacaacccc gcgacaaatc ccgccagcaa cataccgtta
     7081 aatgacgaca ccgttccaag cgccagcagc gaagctaaca tgccgctata aatcagtagc
     7141 gacatggtta ctggacgcag cttatccatc aatgcgccca acattaacgt cccgcttgcc
     7201 gcccccattt gcagggcaaa catcacccct gccgcctgcg atggctggaa tccttgctcc
     7261 accaaaagta gcggtagcca gttgatcaac atataaacca ccagcagagt gaagaaataa
     7321 cacaaccaca gcagcagcgt cgcggttgcc gtttctggcg caaataaggc acgcagtggt
     7381 ggcgcagcct gtttttcgcc agcgaaaacc gctgactccg gcagccagcg cattaatagc
     7441 ggcaccagaa tcaacggcac cacaccacct acccaaaaca ccgtttgcca tgctaagttt
     7501 gcccccgcga aacccagtgt cgccgccagc gccgcgccaa tgggaacacc gcaatacatc
     7561 aggctcactg ccgtcccacg aaaacgtgga cccgcggctt cagacgtcag ggcgataaga
     7621 ttcggcaacg ccgcccccag cccgacaccg gtcatcagcc gcgcaaagac cagtgagggg
     7681 aaatcccagg caatcgccgt tgccagtgag aacaaaccaa acagcgcaac tgagccaatc
     7741 aaaatactct tacgaccata acggtctgcc agcatcccgc caaccaacgc gccgggtagc
     7801 aaaccgagta ttccggcgct aaatatccag cccatttgca ttttatcgag tgcgaaagcc
     7861 tgggcgatgc cacccgccgc aatgccagcc gcctgaagat ccagcccttc catcagagcg
     7921 accaaaaaac aaagcccgat ggtcagcatc aggcgggatg aagatgatga aggggtacga
     7981 gtcgacataa gatgaacctc gttgcgagtg caggtgaatc gcgactgata cattgccagc
     8041 cgcgtaatca gaactattta atgactgcct acgggcgaaa tgtaggccgg acaagacgtt
     8101 tatcgcatcc ggcagtcgtg tgcagatgcc tgatgcgacg ctaacgcgtc ttatcaggcc
     8161 tacaaattct cacgccaccc gtaggccgga taaggtgttt acgccgcatc cggcagtcgt
     8221 gctgggatgg gcatacaaaa tgcacgttct cggcagtcat cattatttgt tgttgcgcag
     8281 atccagcgcc acgtcaacga tcatatcctc ctggccgcca accatccggc gtttgcccag
     8341 ctcaacgaga atatccaccg cacttaagcc ataacgcgcc gccgccgttt cacagtgacg
     8401 caggaagctc gagtaaacac cagcgtatcc cagcgccagc gtttcgcgat cgactcgtac
     8461 cggtcgatcc tgcaacggac gcaccaggtc gtcagcggca tccattaacg catagagatc
     8521 ggtcccatgc tgccagccca gtttatccgc ggcggcaata aacacttcca gcggtgcgtt
     8581 acctgcgccc gcgcccattc ccgcgaggct ggcgtcgatt cggtcgcagc cctcttccac
     8641 cgccgcgata gagttcgcca cgccaagact caggttatgg tgagcgtgca tgccagtttg
     8701 cgtttctggt ttcagctctg ctttcagggc gcggaaacgg tcacggatat cgctcatgtt
     8761 catcgcaccg ccagaatcca ccacataaat acaggtcgca ccgtagcctt ccatcagctt
     8821 tgcctgcttt gcgagattct ccggcgtggt catatggctc atcatcagaa aaccaacggt
     8881 gtccattccg agctcgcggg catactgaat atgctgggcg gaaacatcag cttcggtaca
     8941 gtgcgttgcc acacgaacca cccgcgcgcc agcctgccaa gcatttttca gatcgtgaat
     9001 agtgccgatt cctggcagca acaacgtcgc gattttggcg tgcttcacca catccgccgc
     9061 tgcttcaatc cattcaaggt cgctatgtgc gccgaaacca tagttaaagc tggaaccctg
     9121 caaaccgtcg ccatgggcca cttcaatcga atccacgcgg gcatcgtcca gtgctttggc
     9181 aatctggcga acgttttcca gcgaatactg atgacgaatg gcgtgcatac cgtcacgcaa
     9241 cgtgacgtcc gagatataaa gttttttacc gttcatgccg cttctcctgc cttgcgcgcc
     9301 agtgactggg ccattttttc cgctgtcgcc agcgcactgg aagtcataat gtcgaggttg
     9361 cccgcatagg caggcagata atgcgctgcg ccttcgactt ccagccagac cgctgttttc
     9421 agtccggaga attgccccac gcccggtaaa ttgaccggtt tatcctgcgg gataacttca
     9481 aactgcacgc gctgtttcag gcgataaccc ggtacgtaag cctgcaccgc ctcagccatt
     9541 tcattgattg aggcttcgat atcatcttgt gaagcttcgt cgctcaatac atacaccgtg
     9601 tcacgcatca tcaacggtgg ctctgctggg ttaagcacaa taatcgcctt cccttttgcc
     9661 gcgccgccca ccacttcaat ggctcgggaa gtggtttccg taaattcatc gatattggca
     9721 cgcgtgccag gtccggcaga tttactggcg atagaagcga taatttcggc gtaatgaaca
     9781 cgcgccacgc gtgaaactgc cgccaccatt ggaatggtgg cctggccgcc gcaggtgacc
     9841 atgttgacgt tcagttgatc gacgttcgcc tcgaggttaa ccaccggcac gcagtaaggg
     9901 ccgatggcag caggcgtcag gtcaattaag cgaatatccg gtttcgcttc gcgtaaagcg
     9961 gcatcgtttt tcacatgcgc gcccgcgctg gtcgcatcaa atacaatgtc gatatcagca
    10021 aattcaggca tgttcatcag cccgatcacc ccttcatggg tggtggcgac gcccatacgt
    10081 ctggcacgcg ccagaccgtc ggactgagga tcaatgccga ccatcaccgc catctccaga
    10141 tgctgaccgt gacgcaaaat tttaatcatc agatcggtgc caatgttgcc agaaccgata
    10201 atggcgactt tacgcttact catgacagac ttccttttgg ggctgcgctt gaaaatgtcg
    10261 cagcaactga acctatgcct tcaatatggg cttcaaaacg atcgcccgca ttcactgcca
    10321 ccatcggacc taatgccccg gtaagaatga tatctccggc gcgcagcggt tcacccaggc
    10381 tggccatttt gcgtgccagc cagacggcgg cattaagcgg atgtcccagg cattcgctgc
    10441 cgcgcccgct ggaaacctct tcgttattac gcgtcatctt catggcgcag tttttcaggt
    10501 ctaaccccgc cggacgttgc gccggaccgc cgatgacata caccccgcag gaagcgttat
    10561 ctgccacggt atcgacaaac tgaatcgacc agtcgcgaat gcggctaccc accacctcca
    10621 gcgccggaag tacccattca atggcgttat acagttcgtc gaaggtgata tcggttgcgg
    10681 gcaaatcgcg gttcaacacc agtgcgatct ccgcttcaat gcgggcttgc agaacacggg
    10741 aaaaaggaat gatttcgtta tcgccataac acatgtcggc aaataatgtc ccaaaatccg
    10801 gttgatcaac gcccagttgt tgctgcactt tcggatgcgt caggcccact ttacgcccta
    10861 ccacgcgacg cccctgcgca acgtcatatt gcacatttat gtgctgaatg gcgtaggccg
    10921 cttcagcgtt atcgatacca atcagatcgc gcagcggcgc aatcgcttcg ccctgctctg
    10981 cggcgcggcg taaatccgcc gccagttgct caagagtatg cttcgtcatg accattcctt
    11041 aagggcgtgc gaggaaattc agcaccaatt gattgaaagc gtcggcatgt tcccactgcg
    11101 cccagtgacc acagtcgcgg aagatatgca gttcagaacc ggcaatgccg gacagcagac
    11161 gcagacccgc atccatcggc acaaagcggt cgttgcgccc ccagacaatc agggtttgcg
    11221 ctttgatttc cgccagacgt gggccaaaat ccgggaactg tttcggatta gcttccaggc
    11281 tcttaacgaa gttttccagg tgatcgcggc gcgacagcat attattcagg cgcgcttcaa
    11341 acagggcgtc ggtcaaatcg ctggtatcga aaacgaagat atccatcatc agcttcaggt
    11401 tttcgatagt cggctgacga taaagctgat tcagtcgctt aataccttcg gttggcatcg
    11461 gcgtaaacaa actcatgccg cccgtaccgc cgcccatcag caccagtttg ccgacgcgct
    11521 ccggccagtt aagggtgaac gccatagaac tatggccgcc catcgagttg cccagcaggt
    11581 ggattttggc gatatccagt tgatccacca cgcttttcag gattcgtgca ttaagatccg
    11641 atcgtgaacc actattaacg atcgaatcgc ttttgcccca acccggacaa tccagcagga
    11701 ttacccgata gcccgcctct accagcggat cgatattgcg gctgaagttc gcccagccag
    11761 tagcacccgg gccggaaccg tgcagcagga caacggtttc gtcgccttgt ccgcagtcat
    11821 taaaatggat gcgcagcgtt ttacccgctt cttctacatt cagaaaacgg ctggtggcgg
    11881 cttcggtttg tggctgataa ctcatcatct tctcctgcat attcagttct ctgttctggc
    11941 gcttaacgag ccaaatccgg caatccactc cgggattggg cggtaataac gcccttcgct
    12001 acgccagttg ccaaatgcag aaatagcggc aaaagcggcg acccaggttt tgatttcatg
    12061 tgtcgacttt ccggcaatgg cggaaagctc ttcgttactg acggcatcca gttcctgtat
    12121 gcgtccctgc tccagcaaag tcatgaactg gttatcccaa atcgggttga gcggatgcag
    12181 agttctctga tcctcaacaa acttctcagc ggcgctaatc acccgttgct gacgcaattc
    12241 gcgctcactg gcgggtaaat ctttcccgct ccccaacaga cggtcgcgca tatgggcatc
    12301 ggctttcgcc agttcgggca ccggcggctg atgggaaagc ccaccggaac ccaggaacag
    12361 cacgcgttta ttgagagtgc tagtgaaacg tccaatggct tcacccaaca tgcgggtacg
    12421 ctggaaaccg ggcagcggcg tggcgacacc gttgatgaac acaggcagaa ctggcacctt
    12481 atccagcccg ccgagcagga actccagtgg ctgggcgaac ccgtggtcca cctgcataca
    12541 gtaagaaacg gcaagatcga tcccgctctt catgacggca tgcgcacagg cctccgccag
    12601 ctccacaggc actggcagct ctcctgccgc actgccgaaa tcaccaattg ccgtcgctcc
    12661 aacgcctaaa cagaacggtg gcatcacgtc atagaaaaag ccgttgtagt gatctggcgc
    12721 aaacagcacc accagttcag gggagaaagc cgcaatacgc tcgcgggcgc tggcaatcac
    12781 gccattgacc tcatcgagca cctcttgcgc cgggtcgaca taccccacca gcggcgagtg
    12841 ggaaagacag tgaagataag cgtgcatatc aggctacctt ttcgacagaa acgtcggcat
    12901 cagggcgggt cagcgtcatc accgacgcca gtttattcag ggtcttgccc agggtttgcg
    12961 gaatggcggt ggcggcaaca aagcgatccg ggcgcatcac caccagcgaa gcattgtgtt
    13021 gcgcgaacca gctacgcaga cgaccttgcg tatcgcccac gcgtagcacg ccgtcgtggt
    13081 tatcctgtgc ggtatgaatt tgcacttccg gcaccacctg aatgaagcgt gtgcccaacg
    13141 cgcgccactg ctggatttgc tcgtcgctca tcccccacag tggattgcat ccccagccaa
    13201 ttaccgcgaa gttcgcgcca atcgcgttat cgagcagcgt cacgtcaccg ttttccagcg
    13261 tgactttcgg ctgaataaac atcttgccga ccggagagtg cttcgcttcg ccctcacgca
    13321 ccagcgcacc gccgtaatat tgcggcatcg gcttgaagcg catttcgagg aaatagcgtt
    13381 ttactggcgg cagataattc aacagccagg aaacgccgtc acgtaacgta ccctgccagc
    13441 gtttcggcgg agccagcacg ttgcccgccg tcacggacag gtcaatcatc gctttggcgt
    13501 gatcgcgacg ttcttgttga taggtatcga gcagcgcatc gcgggctttc ccctggataa
    13561 ccaacgccag tttccaggcg aggttaaagg cgtcgcgcat accactgtta tagccctgcc
    13621 cctgccacac cggcatgatg tgcgcggcat cgcccgccag cagtacgcga tcaatacgga
    13681 aacgttgcgc cagtcgcgcg ttatgggtgt agacacgctg gcgaatcaat tcaacattgt
    13741 ccggattagg cagcactttg cttaacagct tgcgcatatt ttgcggctca cgcagctgct
    13801 cttcggtttc tcccggcatc accataaatt caaagcgacg taccgcatga ggcagcgcgg
    13861 cagaaacata cgggcgcacc ggatcgcaac acaaatagat atgcggcgta cttaacggat
    13921 cgttggcgat atctaccaca atccactgat ttggcgcagt tttaccttca aacggcacat
    13981 tcagagtgcg acggacaaaa cttgctccac cgtcacaggc taccagccac tgggctttga
    14041 ccgtttcccg ctgcccttct gccgttttca ggtgcaaggt cacttcgtca ttttgctggc
    14101 tgaaggcctc cagctcgcgg gaaaacaagc agcgcacatt cggaaaacgc gacaaccctt
    14161 ccagcatcac cgcatcgacc tgaggctgaa taaaggcgtt acggcgcggc cagccaaatt
    14221 catcggtcat tggctgaata tcagcaaaac agcggccttt cggggtgaga aaacgcatcg
    14281 cgtgccacgg cgtagtgtgc ggcagaacat catcgaccag gccgaccgac tgcatggtgc
    14341 gcagcgcctc gtcatcaata ccaatcgcac gcgggtagtc gatcaactta tcgagttttt
    14401 ccaccaccag cacgtcaatg cccatctggc cgagatagtt cgccatcatc agcccgaccg
    14461 gaccggcacc agcgatcgcc acctgaacgc tatggttaac agcaggctgg atgtcagggt
    14521 gttgtattgc catttcagta cctcacgact cggacaaaat gccgttgcgc gcacagtacg
    14581 gcgcaactta ttttgttaaa aacatgtaaa tgatttttta ttgtgcgctc agtataggaa
    14641 gggtgttttc ggctacaatc aaaacatgcc cgaatgtgca ccaggtgcac aactttgttt
    14701 taactataga aatgtcaatt aatatgcaga acaatgagca gacggaatac aaaaccgtgc
    14761 gcggcttaac ccgcggtcta atgttattaa atatgttaaa taaacttgat ggcggtgcca
    14821 gcgtcgggct gctggcggaa ctcagcggcc tgcatcgcac cactgtgcgg cgactgctgg
    14881 agacgctgca ggaagaggga tatgtccgcc gtagcccctc cgatgatagt tttcgtctga
    14941 ccatcaaagt gcggcaatta agcgaaggat ttcgtgacga acagtggatt tctgcactgg
    15001 cggccccact gctgggcgat ctgttgcgcg aagtggtatg gccgacagat gtgtccacgc
    15061 tggatgttga tgcaatggtg gtacgcgaaa ccactcaccg tttcagccgc ttatcctttc
    15121 accggccaat ggtcgggcga cgtttgccgc ttctgaaaac cgcctctggc ctgacctggc
    15181 tggccttttg cccggaacaa gaccgcaagg aattaatcga aatgttagcc tcccgccccg
    15241 gtgatgacta tcaactggca cgggaaccgt taaagctgga agccattctg gcgcgcgcgc
    15301 gcaaagaggg ttacggacag aactaccgcg gctgggatca ggaggagaag atcgcctcta
    15361 tcgccgtacc gctgcgcagt gaacaacggg tgattggctg tctgaatctg gtgtatatgg
    15421 cgagcgcaat gaccattgaa caggcagcgg aaaagcatct tccggcgcta caacgggtag
    15481 caaaacagat cgaagaaggg gttgaatcgc aggctattct ggtggccgga cggcgaagcg
    15541 gcgtgcattt acgttgacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag
    15601 cgtccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgct atacgatgtc
    15661 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
    15721 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
    15781 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
    15841 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgctgatcaa
    15901 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
    15961 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
    16021 gaccaggatg ccactgctgt ggaagcagcc tgcgctaatg ttccggcgtt atttcttgat
    16081 gtctctgacc agactcccat caacagtatt attttctccc atgaagacgg tacgcggctg
    16141 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
    16201 agttctgtct cggcgcgtct gcgtctggcg ggctggcata aatatctcac acgcaatcaa
    16261 attcagccga tagcggaacg ggagggcgac tggagtgcca tgtccggttt tcaacaaacc
    16321 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
    16381 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
    16441 gtagtgggat acgacgatac cgaagacagc tcgtgttata tcccgccgtt aaccaccatc
    16501 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
    16561 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
    16621 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    16681 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    16741 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtga
    16801 aattgtgagc ggataacaat tttacacagg atacagctat gactatgatt acggattcac
    16861 tggtcgtcgt attacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    16921 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    16981 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
    17041 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
    17101 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca
    17161 ttacggtcaa tccgcctttt gttcccgcgg agaatccgac gggttgttac tcgctcacat
    17221 ttaatattga tgaaagctgg cttcaggaag gccagacgcg aattattttt gatggtgtta
    17281 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagccgtt
    17341 tgccgtctga atttgacctg agcacatttt tacgcgccgg agaaaaccgc ctcgcggtga
    17401 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
    17461 gcattttccg tgacgtctcg ttgctgcaca aaccgagcac acaaatcagc gatttccatg
    17521 ttgccactca ctttaatgat gatttcagcc gcgcggtact ggaagcagaa gttcagatgt
    17581 atggcgagct gcgcgatgag ctgcgggtga cggtttcttt gtggcagggt gaaacgcagg
    17641 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
    17701 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga
    17761 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
    17821 ccagcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
    17881 agccgttgct gattcgcggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
    17941 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
    18001 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
    18061 tgtatgtggt agatgaagcc aacattgaaa cccacggcat ggtgccaatg aatcgtctga
    18121 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
    18181 atcgtaatca cccgagtgtg atcatctggt cgctgggaaa tgagtcaggc cacggcgcta
    18241 atcacgacgc actctatcgc tggaacaaat ctgtcgatcc atcccgcccg gtgcagtatg
    18301 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
    18361 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
    18421 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
    18481 gcggcttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
    18541 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
    18601 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
    18661 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
    18721 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
    18781 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
    18841 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
    18901 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
    18961 catggtcaga agccggacac atcagcgcct ggcagcagtg gcgtctggcg gcaaacctca
    19021 gcgtgacact cccctccgcg ccccacgcca tcccgcaact gaccaccagc gaaacggatt
    19081 tttgcatcga gctggataat aagcgttggc aatttaaccg ccagtcaggc tttctttcac
    19141 agatgtggat tggcgatgaa aaacaactgc tgaccccgct gcgcgatcag ttcatccgtg
    19201 caccgctgga taacgacatt ggcgtaagtg aagcgacccg tattgacccg aacgcctggg
    19261 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc ggcgttgttg cagtgcacgg
    19321 cagatacact tgccgacgcg gtgctgatta ccactgtcca cgcgtggcag catcagggga
    19381 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
    19441 ccgttgatgt tgaagtagcg agcgatacac cgcatccggc gcggattggc ctgacctgcc
    19501 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc
    19561 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
    19621 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
    19681 gctcacacca gtggcgcggc gacttccagt tcaatatcag tcgctacagc caacaacaac
    19741 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
    19801 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
    19861 tccagctgag cgccggtcgc taccattacc aattggtctg gtgtcaaaaa taataataac
    19921 cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa
    19981 acacaaactt ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct
    20041 acttcccgtt tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg
    20101 gtattatttt tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc
    20161 tttctgacaa actcgggctg cgcaaatacc tgctgtggat tattaccggc atgttagtga
    20221 tgtttgcgcc gttctttatt tttatcttcg ggccactgtt acaatacaac attttagtag
    20281 gatcgattgt tggtggtatt tatctaggct tttgttttaa cgccggtgcg ccagcagtag
    20341 aggcatttat tgagaaagtc agccgtcgca gtaatttcga atttggtcgc gcgcggatgt
    20401 ttggctgtgt tggctgggcg ctgtgtgcct cgattgtcgg catcatgttc accatcaata
    20461 atcagtttgt tttctggctg ggttctggct gtgcactcat cctcgccgtt ttactctttt
    20521 tcgccaaaac ggatgcgccc tcttccgcca cggttgccaa tgcggtaggt gccaaccatt
    20581 cggcatttag ccttaagctg gcgctggaac tgttcagaca gccaaaactg tggtttttgt
    20641 cactgtatgt tattggcgtt tcctgcacct acgatgtttt tgaccaacag tttgctaatt
    20701 tctttacttc gttctttgct accggtgaac agggtacgcg ggtatttggc tacgtaacga
    20761 caatgggcga attacttaac gcctcgatta tgttctttgc gccactgatc attaatcgca
    20821 tcggtgggaa aaacgccctg ctgctggctg gcactattat gtctgtacgt attattggct
    20881 catcgttcgc cacctcagcg ctggaagtgg ttattctgaa aacgctgcat atgtttgaag
    20941 taccgttcct gctggtgggc tgctttaaat atattaccag ccagtttgaa gtgcgttttt
    21001 cagcgacgat ttatctggtc tgtttctgct tctttaagca actggcgatg atttttatgt
    21061 ctgtactggc gggtaatatg tatgaaagca tcggtttcca gggcgcttat ctggtgctgg
    21121 gtctggtggc gctgggcttc accttaattt ccgtgttcac gcttagcggc cccggcccgc
    21181 tttctctact gcgtcgtcag gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg
    21241 cgagcgcctt atccgaccaa catatcagga cggaatgatc gcaatgaaca tgtcgatgac
    21301 agaaagaata aaagcaggca agctatttac cgatatgtgc gaaggcttac cggaaaaaag
    21361 acttcgtggg aaaacgttaa tgtatgagtt taatcactcg catccatcag aagttgaaaa
    21421 aagagaaagc ctgattaaag aaatgtttgc cacggtaggg gaaaacgcct ggatagaacc
    21481 acctgtctat ttctcttacg gttccaacat ccatataggc cgcaattttt atgcaaattt
    21541 caatttaacc attgtcgacg actacacggt aacgatcggt gataacgtat tgattgcacc
    21601 caatgttact ctttccgtta cgggacaccc tgtacaccat gaattgagaa aaaacggtga
    21661 gatgtactct tttccgataa cgattggcaa taacgtctgg atcggaagtc atgtggttat
    21721 taatccaggc gtcaccatcg gggataattc tgttattggc gcgggtagtg tcgtcataaa
    21781 agacattcca ccaaacgtcg tggctgctgg cgttccttgc cgggttattc gcgaaataaa
    21841 cgaccgggat aagcaatatt atttcagaga ttacaaagtt gaatcgtcag tttaaattat
    21901 aaaaattgcc tgatacgctt cgcttatcag gcctacaaat tcagtgatct acgtaggccg
    21961 catccggcgt gaacaaagcg cagaaacaag agtcgcatca tgcctctttg acccacagct
    22021 gcggaaaacg tgctggtgca aaacgcaggg ttataatcat cagcccaacg acgcacagcg
    22081 catgaaatgc ccagtccatc aggtaattgc cgctgatact acgcagcacg ccagaaaacc
    22141 acggggcaag cccggcgatg ataaaaccga ttccctgcat aaacgccacc agcttgccag
    22201 caatagccgg ttgcacagag tgatcgagcg ccagcagcaa acagagcgga aacgcgccgc
    22261 ccagacctaa cccacacacc atcgcccaca ataccggcaa ttgcagcggc aaccagataa
    22321 agccgcagaa ccccaccagt tgtaacacca gcgccagcat taacagtttg cgccgatcct
    22381 gatggcgagc catagcaggc atcagcaaag ctcctgcggc ttgcccaagc gtcatcaatg
    22441 ccagtaagga accgctgtac tgcgcgctgg caccaatctc aatatagaaa gcgggtaacc
    22501 aggcaatcag gctggcgtaa ccgccgttaa tcagaccgaa gtaaacaccc agcgtccacg
    22561 cgcggggagt gaataccacg cgaaccggag tggttgttgt cttgtgggaa gaggcgacct
    22621 cgcgggcgct ttgccaccac caggcaaaga gcgcaacaac ggcaggcagc gcccaccagg
    22681 cgagtgtttg ataccaggat tcgctatgtt gaactaacca gggcgttatg gcggcaccaa
    22741 gtccaccgcc gcccatcaga gccgcagacc acagccccat caccagtggc gtgcgctgct
    22801 gaaaccgccg tttaatcacc gaaggcatca ccgcctgaat gatgccgatc cccaccccac
    22861 caagcagtgc gctgctaagc agcagcgcac tttgggggta aagctcacgc atcaatgcac
    22921 cgacggcaat cagcaacaga ctgatggcga cactgcgacg ttcgctgaca tgctgatgaa
    22981 gccagcttcc ggccagcgcc agcccgccca tggtgaccac cggcagagcg gtcaacaggg
    23041 cagccacgct aaagctcatt ccgctcgcct ggcgcaattg cggtagcagt ggcccgacgg
    23101 aggtgagcag tggtcgcata ttaagaccaa tcagcaccag aaccagcagc atcgtgaacg
    23161 tcttttgatg attgcgcatg gctgtcagaa cggcttggtc ggcagatatt taccatctaa
    23221 ggtaatgacc gcacgttcgc caccttccgg gtccgccact ttcttaacgt cgagtttgaa
    23281 gttaatcgcg ctgataatgc catcgccaaa cttctcatga accaacgctt tcagggttgt
    23341 gccgtacacc tgcaacattt catagaaacg atacatcgtt gggtcggttg gaatacggtc
    23401 atcaatgcag ccacgcagtg gaatcatctg caacagtaga atggcgtctt cgtcgagatc
    23461 cagcttcgcc ccgaccaggc gggcggcgtc ggcaggaagc gcttgctgcc ccagcaaagc
    23521 cgcggttacg aaggcttctg ccagaccggt accgtcggca atctcggcaa atgagagatc
    23581 ttttttagct ttgctgagca aaatggcatc ggcaagatca agacgaatat tgcggttaat
    23641 ttgtgactga atcatggtgg atctcctgat ggtttaaaaa taaggttacg ctgcggtcgg
    23701 ttggcgtagc ggtatggcac aaacgcgagg attagcggcc agtggcacaa actggcgggt
    23761 tgcgccgtca aaagctgcga tgctgccgct ttcaatgtcg tagacccagc cgtgcagggc
    23821 gatccgcccc tcttcgagcg ccaggcgcac cgatggatga gtttgcaaat tagccaactg
    23881 agcaatgacg ttttcacgta ccatcgccgc agcttttgac ggtaaatcgg aatgcgggcg
    23941 cgcctcatta acgacgcggg ctgaatcggc ataacgcagc cagtgggaga cggcaggcat
    24001 atggtccatg cactgacagc tggcaatggc ggtcatcgcg ccacagttgg aatgaccaca
    24061 aatcacaatg tcagataccc gaagcgcagc gacggcatac tccaccgaag cagaaacgcc
    24121 accgggttcc ggcccgtagg aagggacgat attgcccgcg ttgcgaataa cgaacagatc
    24181 gccaggctca cgttgcgtca ccagctcagg gaccagacgg ctgtcggagc aggagataaa
    24241 aagtgtgcgc gggctttgct gtgtcgccag ctgtttaaac aaggcttccc gcttcggaaa
    24301 tgcctcgcgc tggaatttaa ggaatccatc aataatctct ttcaccgtta acctctgtct
    24361 gtctctggaa tgagaggcag actactcgcc gattgtcata aggtaaaagt ctcatttatg
    24421 atgagttcta ttggatttac ttataggttg gcgaatgctc tctcgacata tcaattactt
    24481 tcttgccgtg gctgaacatg gcagcttcac ccgtgccgcc agtgcgttgc acgtctccca
    24541 gcctgcgctt tcccagcaga ttcgccagtt agaggagagt ttgggcgtgc cgctgtttga
    24601 ccgtagcggg cgaacgattc gtctcaccga tgcaggagaa gtctggcgac agtacgccag
    24661 ctgggcgtta catgaactgg gagcgggtaa acgggcgatt catgatgttg ccgctctgac
    24721 gcgaggatcg ctgcgtatcg ccgtcacccc cacctttacg agctacttta tcggcccctt
    24781 aatggcggat ttctatgcgc gctaccccgg catcacgctc cagctacagg aaatgtcgca
    24841 ggagaaaatc gaagatctgc tttgtcgcga cgagctggac gttgggattg ccttcgcgcc
    24901 tgtgcattcg ccggagctgg aggcaattcc tttactgaca gaaagtttag cgttggtggt
    24961 ggcgaaacat catccgctgg ctgcctgtga acaggtggcg ttgagtcgct tgcatgatga
    25021 aaaactggtc ctgctcagcg cggaatttgc caccagagag caaattgacc actactgcga
    25081 gaaagcgggg ctacatccac aggtggtcat tgaggcgaac tcaattagcg cggttctgga
    25141 gctgattcgc cgcacttccc tttccacatt gttaccagca gcgattgcca cacaacatga
    25201 cgggcttaaa gcgatttctc ttgccccgcc actactggag agaacggcgg ttttgttgcg
    25261 gcggaaaaat agctggcaga ctgccgctgc gaaggcattt ttgcacatgg cgttggagga
    25321 atgtgcggat gttggcgaga atgaatcacg gtagccactg acacaggtca gtggctcttc
    25381 aacacagagg aaattaccgt ttgtaatcga tggcttctgg ctgctccaga tatacggtgg
    25441 tttgtgccgg ttgtgtactg gcaatcacct tgccgccacg taccgaataa cgtaccggaa
    25501 cctgacggcg cagcgcatca aatccatttt cagccggcag gataatcagg tttgcgctgt
    25561 ttccggcggc aatgccgtaa tcctgcaaat tcaatgttct ggcactgtgg tgggtgatta
    25621 aattcaggcc atcgttaatc tgcccgtagc ccatcaactg gcaaacatgc agccccatat
    25681 gcagcacttg cagcatattc gccgttccca gcggatacca cggatcgaag acatcatcgt
    25741 gaccaaagca gacgttaatg ccggactcca gcatctcttt aacgcgcgtg atgccgcgac
    25801 gttttggata cgtatcgaaa cgtccttgca gatgaatatt gaccagcggg ttggcgacaa
    25861 agttaatacc ggacattttc agcaagcgga acaggcgtga ggtatacgcc ccgttatagg
    25921 agtgcattgc cgtggtgtgg ctggcggtga ctcgcgcgcc catgccttca cggtgcgcca
    25981 gggcagcaac ggtttcgaca aagcgcgact gctcgtcatc gatctcatca cagtgaacgt
    26041 cgatgagacg gtcgtatttt tgcgccaggg cgaaggtttt atgcagcgac tccacgccgt
    26101 attcacgggt aaattcaaaa tgcggaatcg cccccactac atctgcccct aagcgtaacg
    26161 cctcttccag caacgcttca ccgttgggat acgacaaaat cccttcctga gggaaggcga
    26221 caatttgcag atcaatccac ggcgcgactt cctgcttcac ttccagcatt gctttcagcg
    26281 cagttagcgt tgcatccgaa acatcgacat gggtacgcac atgctgaatg ccgttggcaa
    26341 tctgccattt cagcgtttgc caggcgcgtt gtttcacatc gtcatgggtt aataacgctt
    26401 tgcgctcggc ccagcgttca atgccttcaa acagcgtgcc ggactgattc cagttcggtt
    26461 gtccggcggt ttgcgtggtg tccaggtgaa tatgcggctc cacaaacggc ggtataacta
    26521 aaccttgttc ggcatccagg ctgttttcag ttatgggcat cacgccggat tgcgcatcaa
    26581 tggcgctgat ttttccgtcc tgcagatgaa tctgccacag cccctctttg cctggtaacc
    26641 gggcgttaat aattgtttgt aaagcgttat tcgacactgt taacctccac atgcgtcatt
    26701 gctgctgtcg ttttacgatt caaaatcggg ttaaggatca gatagctcag cgcgccacct
    26761 aataccgcgt tgaccggaac aattcccggt aaccagtggc ctgcggcaat ccccagcgcg
    26821 accgccagaa tcgccaccca attgacactc atcatacgcg tggtcgcaaa gtgctcatag
    26881 cggcgacggt tcatcagata gtcggcgatg atcacgcccc ctactggagg aatagctgcc
    26941 gaaaggaagg tcaaccagcc gacaaaattg ttatacagcc ataatgcgca gaccgtaccg
    27001 ataataccgt tgattaccga aagggttttg ctcgacatac cggtaatgtt ggcgaaacct
    27061 aaacccgacg catagagtgc gttatcgttg gtggtccaga tattcagccc cagcaccaca
    27121 atcgcaggca gcagcaggcc ctgagcaatc atcacatcag agatatccgc catgcccagt
    27181 gccgcagcgc ccgctgcacc gaaaataaac atcaacgagt tgccgaggaa aaaggccacc
    27241 atcgccacca gcaccgccag tttggcattg cgaccaaacc ggacaaagtc agcggtgagc
    27301 gtacccgcac tgataaatga ccccacaacc agcgccagcg cgacattgaa atctaacggt
    27361 tgtgcgggaa cgaccgcttt taatgcgtcc aggccgccca tgccgttaac agccagccac
    27421 acggaataac cgcccaggca ggcgatagcc ggaaccgcaa tcaccgaaag aaccgtcagc
    27481 gccgaaatgc caaaaaagac ggtgacggtc atcagtaaac cggaaacggc aatcagcaaa
    27541 ttaatatcca gcccggttgc cttacccacc ggaatggcaa acatcgccac accgacgcca
    27601 aaccagccaa cctgagttcc gcccagtagc agtgaaggca gccatgagcc tttaacacca
    27661 aacgagaagc gagcaagaag atgagtggtc aggccggttt ttgcgccaat gtaaccgaga
    27721 aatgaagtgt aaataccgag gagaagatta ccgatgagaa ctgcgaggaa gaaatcatga
    27781 tagctaagac cggttccgag agtgccgccg gtccacatac tggcggaaaa gaaggttaat
    27841 cccagcatga cgaacgtcaa tgccaatacc cctttccgcg ccgactgcgg gactggcccc
    27901 tggctaaagt tgttatcttg cgacacgaaa ttcccccttt ttgctgtttg aaacccaaaa
    27961 aatccgccgc attctattca tctgaagata aaaagcaatc gttttcgtgg ggaaatatat
    28021 tttttatatg aatgagtgca aaaaggtggg ggaaaaatta tcggtgtaaa aacagtgggt
    28081 agtgggtagg cctgataaga cgcgtcagcg tcgcatcagg catcggcgca tggtgtcgga
    28141 tgcgccacgc gacgcatccg acaatacctt actcttccat cgcctggcgg atctgatcca
    28201 atgacgcagg atcatcaatg gtcgtcagat ccccaggatc gcgtccttcg caaattgcct
    28261 ggatcgtgcg gcgcagcatt tttccggatc gcgttttcgg caattgcgag acaaaccaga
    28321 cgtgcgccgg gcgaccaaag ttgccaatct ggctgtccac cagcgccata atcgccttct
    28381 cttgcgaatg cgccacatca cgatcttcca gactatcgct ctctttcgga atgacaaacg
    28441 ccaccgccac ctgccctttc agcgcatctt tcaccccaac caccgccact tcggcaacgc
    28501 ccggatggct ggagatactc tcttcaatct cgcgcgtgcc cagacgatgc ccggcaacgt
    28561 taattacatc gtcagtgcgc ccgagaataa agtgataacc gtcagcgtca cggatgcccc
    28621 agtcaaaggt ggcgtacacc gggcgggaaa acagcgacca gtaagtcttc acaaagcggt
    28681 cgtcgtcgcc ccagatggtc tgaatacagc ccggcggcag cggcccttcc accaccagca
    28741 tccctttctc gttgacgcca cacggttcgc cggtgacttc attaagcaac tgcacgttat
    28801 agccatacat cggcacgccg gggcttccca gacgcgtcgg cctgtcgtcc agaccgcgag
    28861 caatcgccat aatcggccag ccggattcgg tctgccagta gttgtcgatg accggcacat
    28921 ccagcgtatt gctcacccaa ctggcggtcg gctcgtccag cggttctcca gccagataga
    28981 gcacttccag cgacgagaga tcgtgtttgc gaatttcagc ggtagggaat tttttcagca
    29041 cgcgaatggc ggtcggcgct gagaacatcc ggctaacctg atatttctcg acgattttcc
    29101 accacacgcc gcagtccggc caggtcggca atccttcgta aacgatagtc gccatccccg
    29161 ccagcagcgg cgcgtaaacg atatacgaat gccccaccac ccagccgata tccgatgcgc
    29221 aaaagaacac gctgcccgct ttgccgccaa aaatggtgtc catcgaggtc gccagcgcca
    29281 ccgcatatcc gccgacgtca cgctgcacgc ctttaggttt gccggtcgtg ccggaagtgt
    29341 agagaatgca ggaggtttcg ttggattcca gccacgccac cggtacccgc gcgccgatgt
    29401 gttgatggcg caacgacgcg aaatcgacat cccgcccgct gacgcgcgcc attttcgcca
    29461 gcccgcgatc caccagcaaa acatggcgtg gctggtgctg cgcctgactt atcgcatcgt
    29521 cgagcaattt tttataggga atgattttgc caccgcgcgc tccggcatca gccgagacaa
    29581 tcagcaccgg tttagcgtca tcaattcgcg ccgccacgct gtgcgaggca aaaccaccaa
    29641 ataccaccga atgaatagca ccaatgcgcg cgcaggccag cagagtaata tgcgcttcgg
    29701 caatcatcgg catatacacc agcacccgat cgccgcgctg cacgcccagt gagcgcaaca
    29761 ttgaggccac cgcgttcact tcgtcatgca gctggcgaaa ggtaaaggtg cgctcttctt
    29821 ctgtttccga agagacggca atcaatgcca gtgcctctgg ctgtttctcc agccagcggt
    29881 cgatagcgtt gtggcacaaa ttggttcggc cttcacaaaa ccaacgggca aacggcggat
    29941 tgctgtggtc gagcgtttgc gtaaagggcg tctgccagtc aatacgccgg gcctgctcgg
    30001 cccagaactg ctccggttcg ttaatcgaac gctgataaaa ttcgctaaaa gacataatgc
    30061 tctcctgttg aacttacgcc ttaccgccgt ttacttaaat gacgtacagg tcgagatact
    30121 cattgaccgg catctgttcc aggcgagttc tgtcgagaga aacctccaga atgcgctgct
    30181 gttggcgagt cgggaactgg cgcgcgagat tgattttgaa tttatcgacc agtttcggaa
    30241 taccatcctg acggcggcga gcatgaccaa tggggtactc caccaccact tcttcaaatc
    30301 gtgtgccgtc ggtgaactca agggtaatgg cattggcgat ggcgcgtttt tccgggtcgt
    30361 ggtagtcagc ggtaaatgcc ggatcttcaa agcaattgat cttctcgcgc agggcgtcga
    30421 tgcgtttatc ttgcgcaacg ttgtcctcgt aatctgccgc cgttaagcgc ccgaataaca
    30481 gcgggatcgc caccatgtac tgaatgcagt ggtcgcggtc agccgggtta ttgagcggcc
    30541 cctttttgtc gatgatgcga atacaggctt cgtgggtgcg aatggtcact ttttcgatat
    30601 ccgccgccgt cttgcctgct gcctgcatct gttcatagag cgtcatcgcc gcttcaactg
    30661 ccgtctggga gtggaactcc gccgggaagg agattttgaa cagcacattc tccatgacgt
    30721 aagaaccgta cggacgctgg aagcggaacg attcaccttt aaaggagacg tcgtagaagc
    30781 cccataccgg cgcagtcagg gctgacgggt aacccatttc gcccgttttc gccatcagtg
    30841 ccagacgtac cgcgcgggaa gtggcatcgc ccgccgccca ggatttacgc gtgccggtgt
    30901 tcggcgcatg gcgataggtg cgcagcgact gaccgtccac ccacgccagc gaaacggcgt
    30961 tgagaatttc ctcgcgggtc aggccgagca tttcggcgac cacggcggtg gaagccactt
    31021 tcactaacag aacgtggtcg aggccgacgc ggttaaagga gttttccagc gcgatgcagc
    31081 cctgaatttc atgggctttg atcattgcgg tcagcacctg tttcatggtc aacggcgctt
    31141 tgccgctggc gaccgcgttg cgcgaaagcc agtccgccgt tgccagaatg ccgccgaggt
    31201 tgtcggaagg atggccccac tccgccgcca gccaggtatc gttgaaatcg agccagcgga
    31261 tcatcgcgcc gatgttaaat gccgcctgga cggggtcgag ctggaactga gttccgggga
    31321 cgcgcacgcc gttgggtacg acggtgccgg gaacaattgg ccccagcagt tttttacagg
    31381 ccgggtattc gagagcttcc agaccgcagc cgagcgtgtc gagcaggcag taatgtgcgg
    31441 tgtcgtaggc cactttagag ctgatttcgt agttcatgac gtaatcgacg atatcaacga
    31501 tttcacgatc aaattccggg cggatgttgt tgatttgagc tgacattggg tacgtttcct
    31561 tattgttatt cgtagaggtt tactggcgct tatccagcgc gacaaacggg cggtcttccg
    31621 gtccaacata attggcggaa ggacggataa ttttgttgtc ctgacgttgt tcgataatgt
    31681 gcgccgccca gccggtgacg cgggcgataa caaacagtgg tgtgaacatc tcggtgggaa
    31741 cgcccatcat gttgtaggaa acagcagaga accagtcgag attggggaac atctttttgc
    31801 tctcccacat caccgtttcc aggcgatcgg cgatgttgta catcttcagc gagccgcctt
    31861 cctgcgagag ctgcttcgcc acacgtttaa tcacctggtg gcgcgggtca gcgatggtgt
    31921 aaaccggatg accaaaacca atgaccactt ctttgttttc cacgcgcttg cggatatctg
    31981 cttcggcttc gtccggcgtt tcgtagcgtt gctggatctc cagcgacact tcattcgccc
    32041 cgccgtgttt tggcccgcgc agtgcgccaa tcgcgccaat aatcgcggaa tacatatcag
    32101 agccggtgcc cgcaatcacc cgactggtaa aggtggaggc gttaaactcg tgttcggcgt
    32161 acagcaccag cgagatatgc atcgcctttt cccagctttg cgatggcttt tcgccgtgca
    32221 gcagatgcag gaagtgaccg ccgatggagt cgtcatcggt ttccggctgg atgcgttcgc
    32281 cgttgtgact gtagtgatac caataaagga gaatcgagct aagtgatgcc agcagtttgt
    32341 cggcaatatc ccgcgcgcca gagacagtgt gcccctcttt ttctggcagt gtgcagccga
    32401 gcgcggaaac gccggtgcgc atgacatcca tcgggtgcga cgccgccggt aaggcttcca
    32461 gcacggtacg cacattagct ggtaaaccac gcagggcttt cagtttcgtt ttgtaagcgg
    32521 cgagttcgtc acgggtcggc agtttgccgt ggatcagcag gtgcgccact tcttcaaatt
    32581 cgcaatgttc cgccagatca agaatatcgt agccacggta atgcaggtca ttgccacttt
    32641 tacccacggt gcagagcgcc gtattgcccg ccggaacgcc agaaagtgct accgattttt
    32701 tcggtttaat gacatgggta ctgttttgca ggatcgttgt gtcgctcata ttgtcctcgt
    32761 catttgtagg gtcgagatat ttttcgttgt gttttatttc acctggctac gggcaaacag
    32821 gtcgtcgagc ttctcttcgt actggtagta gttgatgctt tcgtacagct cgttgcgggt
    32881 ttgcatggta tcgatgacgc ttttctgcgt gccttcctga cgcaggacgt tgtagacatg
    32941 ttcagcggcg cggttcatgg cgcggaacgc tgaaagcggg tacagcgcca ttgcgacatg
    33001 ggcgctgcgt aattcgtcgg tggtaaacag cggtgttgcg ccaaattcgg taatgttggc
    33061 gaggattggc acctgcaccg catcggcaaa ctggcggtac atggcgagtt cggtaatcgc
    33121 ctccgggaac aacatctcgg cacccgcttc aacataggcc tgcgcacgct cgatcgccgc
    33181 atccagcccc tctaccgcca gagcatcggt gcgcgccatg atcacaaaat caggatcggt
    33241 tttcgcatcc accgccgcgc ggatccgatc caccatctct tctttcgaga cgatcgcttt
    33301 attcggacga tgaccgcagc gtttcgcacc aacctgatct tcaatatgca atcctgccgc
    33361 accggcttta atcatcgatt tcacggtgcg cgccacgtta aaggccgaag aaccaaaacc
    33421 gatatccgca tccaccagca gcggcagcga acaaacgtcg gtgatacggc gaatgtcggt
    33481 cagcacatca tcaagggtag aaataccgag atcgggcagc cccagcgaac ctgccgccac
    33541 gccaccgcca gaaagataaa ttgcctgata tccggcacgc tgcgccaaca gcgcatgatt
    33601 agcgttgatg gtgccaacaa tctgcaatgg attttcttta gtcagtgcag cgcgaaacgc
    33661 tttacctgga gagtgtagag acatagccca tcctttgtta tcaacttgtt atttgcgttg
    33721 ataaagacaa agcaaggggt gtgccacaat tgccttaata aaattaaatg tttaatttca
    33781 atttgttaga gataaagtca tgaactaatc cgcaaatatg cgtttcagtt aacgtttcag
    33841 gcaatgtttc accgcgtttc attgcaacaa ttatgaaaca agactaaacc caatattcgg
    33901 tttcttaact ttgcggtgcg ctatggcaca tccaccacgg cttaatgacg acaaaccggt
    33961 tatctggacg gtatctgtaa cgcgcctgtt cgagctgttt cgcgatatca gcctcgagtt
    34021 tgatcacctg gcgaacatta cccctattca gcttggcttt gaaaaagcag tgacctacat
    34081 ccgcaagaaa ctggcaaacg aacgctgtga cgccatcatc gcggctggct ctaacggcgc
    34141 gtacctgaaa agccgcctgt cagtgccagt tattttgatt aaaccgagcg gctacgatgt
    34201 gttacaggca ctggcaaaag ccggaaaact cacctcttct atcggcgttg tcacctatca
    34261 ggaaaccatt ccagcgctgg tggcgtttca aaaaaccttt aatttgcgcc tcgatcaacg
    34321 tagctacatt accgaagaag acgcacgcgg gcagattaac gagctaaaag ctaacggcac
    34381 cgaagcggtg gtcggcgcgg ggctgattac cgatctggca gaagaagccg gaatgaccgg
    34441 aatttttatc tattccgccg ccaccgtgcg ccaggcgttc agcgatgcgc tggatatgac
    34501 gcgcatgtcg ttacgccata acactcacga tgccacccgc aacgccctga gaactcgtta
    34561 cgtgctgggc gatatgctcg gtcaatcacc acagatggaa caagtacggc agactatttt
    34621 gctgtatgcc cgctccagtg cagcggtgtt gattgagggg gaaacgggga cgggcaaaga
    34681 gctggcggcc caggcgattc atcgggaata ttttgcccgc cacgatgcgc gacagggcaa
    34741 aaagtcgcat ccgtttgttg cagtcaactg cggggcgatt gccgaatcgc tgctggaagc
    34801 agaactgttt ggctatgagg aaggggcgtt taccggctcg cgacgcggcg gtcgcgccgg
    34861 gctgtttgaa atcgcccacg gaggtacgct gtttctcgat gagattggcg aaatgccgct
    34921 gccgttgcag acccggctgc tgcgggtgct ggaagaaaaa gaggtcaccc gcgtcggcgg
    34981 gcatcagcct gttccggtgg atgtgcgggt cattagcgcc actcactgca atctggaaga
    35041 agatatgcgg caagggcagt ttcgccgtga cctgttttat cggctgagta ttttgcgtct
    35101 gcaattgcca ccactgcgcg agcgggtggc ggatattctg ccactggcgg aaagcttttt
    35161 gaaagtgtct ctggcggcgc tctccgcccc gttttctgcc gcattacgcc agggattaca
    35221 ggcaagcgaa accgtgctgg tgcactacga ctggccgggc aatattcgtg aactgcgcaa
    35281 tatgatggag cgactggcgc tatttttaag tgtggaaccg acgccggatt taacgccgca
    35341 atttttgcag ctgctactgc cggaactggc gcgcgagtcg gcgaaaactc ccgctccacg
    35401 cttactgaca ccacaacagg cactggagaa atttaatggc gataaaacag cagcggcgaa
    35461 ttatttaggc atcagccgga cgacgttctg gcggcggctg aaaagctgaa ctgccgaatg
    35521 cagcgcgaac gctttatccg gcataaattt caatagatga tcaccgataa acttacgcat
    35581 cgggcatgtt tacatagggt tcagaattac ttcttcttat aaatatttgc cgtgccgtgg
    35641 atcttattgt cagtttgacc ggaggtcagt accaacacat cagccccttt ttcatccgct
    35701 tttttgatca aatcttcttt cgcgtctgca gtcgacattt cattgctggt tgaaatatca
    35761 cctatttttt catactgcga ttcaactttt tcaaattccg ctttggtcat caattcagcg
    35821 gcatagacat tggtaacggc taacgctaac gcaccgacta acattttaga gattattttc
    35881 atattacggc tccagagttg ttatcacact atcacccacc cacattattg ggttgattaa
    35941 taattagttc agtcagggca taaacgccag taaccggaaa taagacactt tgtgatctct
    36001 ctcgcacccg attatttttg gtagaaatat gatgaagtta cttcacttat ttatggatga
    36061 aatcactatg gatcctttgc acgccgttta cctgaccgta ggactgttcg tgattacttt
    36121 ttttaatccg ggagccaatc tctttgtggt agtacaaacc agcctggctt ccggtcgacg
    36181 cgcaggggtg ctgaccgggc tgggcgtggc gctgggcgat gcattttatt ccgggttggg
    36241 tttgtttggt cttgcaacgc taattacgca gtgtgaggag attttttcgc ttatcagaat
    36301 cgtcggcggc gcttatctct tatggtttgc gtggtgcagc atgcgccgcc agtcaacacc
    36361 gcaaatgagc acactacaac aaccgattag cgccccctgg tatgtctttt ttcgccgtgg
    36421 attaattacc gatctctcta acccacaaac cgttttattt tttattagta ttttctcagt
    36481 aacattaaat gccgaaacac caacatgggc acgtttaatg gcctgggcgg ggattgtgct
    36541 cgcatcagtt atctggcgag tctttcttag tcaggcgttt tctttgcccg ctgtgcgtcg
    36601 tgcttatggg cgtatgcaac gcgttgccag tcgggttatt ggtgcaatta ttggtgtatt
    36661 cgcgctacgc ctgatttacg aaggggtgac gcagcggtga gcagataatt tactctctac
    36721 gtaatcaacc tgatttgccg aatcccggcc atcggcgatg atagcccccg taatgcgtgg
    36781 tccttatttc cgataagttt acttgagtct ttagcttccc atttcagccc actctcccct
    36841 gtgtgggctt ttttcattgg tctgacccgt cctgaatagc caaacaatgg caatgttcca
    36901 ctttgcaggg atgataataa ggggatctga ttcagaagag ttttgaaaag ttggaccgcc
    36961 atcgaggcct cgaaccccgt accatacaac aatcctggtt gtcgtctgct cttcctgatg
    37021 agctaatggc ggtttttgat atgagcctta cagcatattt tctgtcaggc ttgagtgcga
    37081 tgcgtaagat acagaaaaga ccccacacaa acatgatgga attatgattt ttttcatatg
    37141 aatgtcaacc cacacattca caaaacatct gcatgagatc attttttata caccgcatgg
    37201 ctgcgaaacc actactggtg cttttttaag cgaagtgtcg ccgatcacaa caaaatatcg
    37261 gcgaaaaaag agatggaatg ccagttggca tacagatata cagatgaaat gattacctgc
    37321 attgcgattc actctcaggt ggaataacaa cttgcaggtg gaaccctttc tgaactaaag
    37381 agtgtagcgg attttaacgt gcttatttac aggataatat ctgtcaccta attcctggac
    37441 tctattagat atccattgat ttaatcgcgc atcatctctg tatgttttta cgtccagaac
    37501 agagagcaat aacatcacca gtgattcagg aaaaacaggg tagatatttt ttaagtgtaa
    37561 taataatcca tgcgcaagcg cctgcttaat gggcgcgtct gtctccggaa tagacgacat
    37621 ttcgcgaagc catttttcca catgcataca ctctttccct ttagggaaaa ttctcagcaa
    37681 aggcatatgg tgttcaagaa gaatacattt gcaatctgtt cgcactgaat cgataaactg
    37741 cactaatttc ttaagcccgg taaagcgtcc agcctgcacg tttgctttaa tatcaagaaa
    37801 cgcatcatcc tggtcatata cccaaactgt ccctttttga ttttcaagta tgcgaaaaac
    37861 aaacttaacc gggtgatcag ggtaatccat ccagtcataa aatccaggga aatcattaaa
    37921 ctgcacattt atggtgagtg cgttatcgtc tgaccactca cagtctcgta cggcagccat
    37981 gagatgaatg agtttgtcgt catcgactaa atcatcacga caatcataag gataatcata
    38041 atattgttta atcaaaacct tgaatatatc gacacaggaa accagggagc ttttgatatc
    38101 ctctggtaac tgattgtttt taaccgtatt aagtatgctt tgttgcgtat atggcatcaa
    38161 attattgtgc ggagctttta atgatatcat cgcgatctct cttaaataaa aataaacgtc
    38221 gcagataata aagctctgat taaaccaggc actatcagaa atcgctcata actaacgtgt
    38281 gaagtattgt gtactggagg gagttaattt aataatttga atttacgtta taggttttac
    38341 tgctactttg atattatttc gctctcctta actaaaaagt taagtggaga gcgaagttaa
    38401 tatgttaaac cacagcgtat ttattaattt tttcagtctg ttagtgtgcg attatcgata
    38461 acaaaacgat atttcacatc accgcgcagc attcgctcat aggcttcatt aatttgatcg
    38521 gcccgaatca tctctatatc agccacaatg ccatgttcgg cgcaaaaatc gagcatctcc
    38581 tgagtttctg gaatgccgcc aatcatagaa ccggctatcg cacggcgttt catgatcagg
    38641 ttgaaaactt ccggcgattt atgcggtgtc gcaggcgcac caaccagcgt catggtgcca
    38701 tcacgcttca gcaaggtggt aaaatcgtcg agattatgtg gcgcagctac tgtattcaaa
    38761 atgaaatcga aactcttcag atgagccgcc atctcatcgg cattgcgtga gttaacaact
    38821 tcatcggccc ccagggcttt tgccgcttcg cgttttgcct cagaagtggt aaatgccacc
    38881 acatgtgccc ccatcgcgtg ggccagctta atccccatat gtcccagacc gccgatgccg
    38941 accacgccca cttttttacc cggcccggcc tgccagtgac gtagcggcga atacgtggtg
    39001 atccctgcac acaacaaagg agccaccgcc gccagctgct cttgcgggtg acgaatacgc
    39061 agaacatatc gctcatgaac gacgatctgt tgtgagtagc cgcccagagt atggcccggt
    39121 tcgtccggcg tcggcgagtt ataggtgccg gtcatgtgat cacagtagtt ttccaacccg
    39181 tcttcacact cttcgcaatg tttacaactg tcgacaatgc agccgacacc gaccagatcg
    39241 cccggcgcat atttttctac ctgatcacca acggctacca cacgccccac aatttcatga
    39301 cccggcacgc aggggtaaac cgtccccgcc cactcggaac ggacctggtg gagatcggaa
    39361 tggcaaacgc cacagtaagc gatttcgatt ttgacatcat tcggtcccgg ttcacgccgg
    39421 gtgatatcca tcggttcaag tggttgttta gcggaatatg caccaacagc tttgatcttc
    39481 attgtgttta ctcctgatta gctatgtgta tttggcgacg cctgatatgt catcgcggca
    39541 aaacgcgtcc attgaatata gccaatattt aaaccacccg atccagcccc aacatgccgc
    39601 gagcctgaat gaaacagagc agcaacgttc cccataaggc catcgtcagg taggggctta
    39661 taccgagaat attgaagccg ctttcaataa tttgcagaag aaacagcgcc agcaccatac
    39721 caattatccg cccgctgccg ccgtccggat taaccccgcc gagtactgcc gcgaggattg
    39781 acaccagcaa gtacgattcg ccataagagg ccttagccga gttgagtttc gacatcatca
    39841 aaaatgccgc taccgcacaa agcaaggcgg aaatgacata aacccaaatc agcacccgac
    39901 gagtgttaat gccggagtaa agtgttgctc gttcgttaga cccaatcagg taaatggctt
    39961 taccgagcgg cgttttctcc agcaatatcc atagccccag tgccactgcg gcaaacaacc
    40021 acatcggtag cggaataccg aaccattgcg catgattcaa ccacagcacc catgagggat
    40081 aattggcaat ggcgctacca cctgtcacca gaatatttat ccctttgagc aacgtcatca
    40141 tcccaagcgt tgcaagaatt ggcgacactc gaataccagc aatcaatacg ccgttacata
    40201 aaccgattat caccgccgcg ccagcgcccg ccagtaacgt agcgacaacc gtagcaatac
    40261 ccggtggata ctgagtcgcc acccacgcca tcaccagtga acaggcgtta gcagtggcta
    40321 taattgacaa gttaatgcca ccgcaaagca tggtcaccgc catagccaac gccagaatgc
    40381 ccagcacggg catttgcgaa gcgacggact ggaaattact tatcgaccag aaaatctccg
    40441 gcatagtaat gctgaacgcg gcaacagtta gcgccagcag gccgatcaag taaaactcaa
    40501 cgttattacg ccacgatttt ttcatagcag actccggttc tgatgctgac tccacgccgt
    40561 ggcactaatg ctggcaacga tgatgatgcc ggtgatcaat gtttgccagt aagacgagac
    40621 tcccaataaa tttagcccgt tttgcatcac tgccaacaac accacgccga gcaaagtacc
    40681 ggttaacgtg ccgcgcccgc cgagcaaact agtgccgcca agcaccaccg cagccagtac
    40741 tgtcagctca taacccagaa gagaatcggg ggcgacagtc atcaccgtcc acgactgcac
    40801 tacacccgca gcgccagaca tcaatcccat atagccatag acgaaaagtt gcagtttcag
    40861 cacgctaaag ccgatgcgac tggctgattc gcggttcccg ccaagggcat aaattttgcg
    40921 ccctacactg gtgaaattca tgataaacgc tgttagcagc accaccgtaa tcatggcgat
    40981 cagcggcaga ccaaggccat agtcatagcc atcagcatcg gtgtacttga acaacataac
    41041 cccctgctca aaccacggcg gaaagtcgta cagccacaca cctttactca accataacag
    41101 caggccatag aaaatattca gcgtcgagat agtgatgatg atagaaggca cccgtaggcg
    41161 attaaccagt aaggcgttaa ttaagccaag taaaatgccg atgcagcccg ccagtgcaaa
    41221 cgcgacagcg aagttgccac caattttttg cagcaacagc accatgccgt attgcgcaat
    41281 gatggtcatt gctggaaacg aaatatcaat tccaccggaa atcaacacca caaacaaacc
    41341 acaggcgaga atagtcaaca tggcatagtt attggcgaga tcgtacaaat taccgaatgt
    41401 cagaaactcg tcgctgcgcc aggcaaggcc aacaaacagc accacaatca gtaatgccag
    41461 ccagaattca tggcgttttt tcagttcagc catttaccac ctccgcaatc ctggcctgag
    41521 aactgctgtc aggcaggaag ctatgggtga tctgaccttt ttgcattacc aaaatccggt
    41581 ggctttgata ccacgcttct tcaatttcat cgcagatcat caataccgca atgccatgag
    41641 ccgccagatc gctgataatg ccgtatattc ctgctttgtt ggcgatatca acaccgactg
    41701 tcgggctatc gagaatcaac agtcgtggcc caatcgccag ccatttggcg atcgatactc
    41761 gctgcgcgtt accaccagaa agcgtattca ccggaagatg aggatcagag actttgatgg
    41821 tcaatgcctt gattagcctg tccaccaggt cacgaacttt agtttcactc agaaagccga
    41881 agcgatcttt ta
//
LOCUS       DAZFYC010000031        41070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFYC010000031 DAZFYC010000000
VERSION     DAZFYC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.031"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..924)
                     /locus_tag="ACHKW5_001855"
     CDS             complement(<1..924)
                     /locus_tag="ACHKW5_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7511262.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPA"
     gene            complement(1084..1296)
                     /gene="rpmE"
                     /locus_tag="ACHKW5_001856"
     CDS             complement(1084..1296)
                     /gene="rpmE"
                     /locus_tag="ACHKW5_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7511263.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1499..3697
                     /gene="priA"
                     /locus_tag="ACHKW5_001857"
     CDS             1499..3697
                     /gene="priA"
                     /locus_tag="ACHKW5_001857"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO7511264.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3853..4878
                     /gene="cytR"
                     /locus_tag="ACHKW5_001858"
     CDS             3853..4878
                     /gene="cytR"
                     /locus_tag="ACHKW5_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO7511265.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4970..5929
                     /gene="ftsN"
                     /locus_tag="ACHKW5_001859"
     CDS             4970..5929
                     /gene="ftsN"
                     /locus_tag="ACHKW5_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO7511266.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQADTVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            6022..6552
                     /gene="hslV"
                     /locus_tag="ACHKW5_001860"
     CDS             6022..6552
                     /gene="hslV"
                     /locus_tag="ACHKW5_001860"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO7511267.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6562..7893
                     /gene="hslU"
                     /locus_tag="ACHKW5_001861"
     CDS             6562..7893
                     /gene="hslU"
                     /locus_tag="ACHKW5_001861"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO7511268.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7960..8886
                     /gene="menA"
                     /locus_tag="ACHKW5_001862"
     CDS             7960..8886
                     /gene="menA"
                     /locus_tag="ACHKW5_001862"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO7511269.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8979..9464
                     /gene="rraA"
                     /locus_tag="ACHKW5_001863"
     CDS             8979..9464
                     /gene="rraA"
                     /locus_tag="ACHKW5_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO7511270.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9549..9794)
                     /gene="zapB"
                     /locus_tag="ACHKW5_001864"
     CDS             complement(9549..9794)
                     /gene="zapB"
                     /locus_tag="ACHKW5_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO7511271.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10219..11064
                     /gene="glpF"
                     /locus_tag="ACHKW5_001865"
     CDS             10219..11064
                     /gene="glpF"
                     /locus_tag="ACHKW5_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO7511272.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAVCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            11087..12595
                     /gene="glpK"
                     /locus_tag="ACHKW5_001866"
     CDS             11087..12595
                     /gene="glpK"
                     /locus_tag="ACHKW5_001866"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO7511273.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12731..13741
                     /gene="glpX"
                     /locus_tag="ACHKW5_001867"
     CDS             12731..13741
                     /gene="glpX"
                     /locus_tag="ACHKW5_001867"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7511274.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13838..14584
                     /gene="fpr"
                     /locus_tag="ACHKW5_001868"
     CDS             13838..14584
                     /gene="fpr"
                     /locus_tag="ACHKW5_001868"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO7511275.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14589..15017)
                     /gene="uspD"
                     /locus_tag="ACHKW5_001869"
     CDS             complement(14589..15017)
                     /gene="uspD"
                     /locus_tag="ACHKW5_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO7511276.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(15044..15343)
                     /gene="yiiS"
                     /locus_tag="ACHKW5_001870"
     CDS             complement(15044..15343)
                     /gene="yiiS"
                     /locus_tag="ACHKW5_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO7511277.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15555..15995)
                     /gene="yiiR"
                     /locus_tag="ACHKW5_001871"
     CDS             complement(15555..15995)
                     /gene="yiiR"
                     /locus_tag="ACHKW5_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7511278.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            16096..16695
                     /gene="yiiQ"
                     /locus_tag="ACHKW5_001872"
     CDS             16096..16695
                     /gene="yiiQ"
                     /locus_tag="ACHKW5_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO7511279.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16803..17570
                     /gene="tpiA"
                     /locus_tag="ACHKW5_001873"
     CDS             16803..17570
                     /gene="tpiA"
                     /locus_tag="ACHKW5_001873"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO7511280.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17625..18380)
                     /gene="cdh"
                     /locus_tag="ACHKW5_001874"
     CDS             complement(17625..18380)
                     /gene="cdh"
                     /locus_tag="ACHKW5_001874"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO7511281.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18487..19476)
                     /gene="sbp"
                     /locus_tag="ACHKW5_001875"
     CDS             complement(18487..19476)
                     /gene="sbp"
                     /locus_tag="ACHKW5_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO7511282.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19796..20758)
                     /gene="pfkA"
                     /locus_tag="ACHKW5_001876"
     CDS             complement(19796..20758)
                     /gene="pfkA"
                     /locus_tag="ACHKW5_001876"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO7511283.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20939..21841)
                     /gene="fieF"
                     /locus_tag="ACHKW5_001877"
     CDS             complement(20939..21841)
                     /gene="fieF"
                     /locus_tag="ACHKW5_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO7511284.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21990..22490)
                     /gene="cpxP"
                     /locus_tag="ACHKW5_001878"
     CDS             complement(21990..22490)
                     /gene="cpxP"
                     /locus_tag="ACHKW5_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO7511285.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22640..23338
                     /gene="cpxR"
                     /locus_tag="ACHKW5_001879"
     CDS             22640..23338
                     /gene="cpxR"
                     /locus_tag="ACHKW5_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO7511286.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23335..24708
                     /gene="cpxA"
                     /locus_tag="ACHKW5_001880"
     CDS             23335..24708
                     /gene="cpxA"
                     /locus_tag="ACHKW5_001880"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO7511287.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24814..25488)
                     /gene="yiiM"
                     /locus_tag="ACHKW5_001881"
     CDS             complement(24814..25488)
                     /gene="yiiM"
                     /locus_tag="ACHKW5_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO7511288.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25637..26620)
                     /gene="kdgT"
                     /locus_tag="ACHKW5_001882"
     CDS             complement(25637..26620)
                     /gene="kdgT"
                     /locus_tag="ACHKW5_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO7511289.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26880..27500)
                     /gene="sodA"
                     /locus_tag="ACHKW5_001883"
     CDS             complement(26880..27500)
                     /gene="sodA"
                     /locus_tag="ACHKW5_001883"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO7511290.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27785..28819
                     /gene="rhaT"
                     /locus_tag="ACHKW5_001884"
     CDS             27785..28819
                     /gene="rhaT"
                     /locus_tag="ACHKW5_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO7511291.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGLSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28816..29664)
                     /gene="rhaR"
                     /locus_tag="ACHKW5_001885"
     CDS             complement(28816..29664)
                     /gene="rhaR"
                     /locus_tag="ACHKW5_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO7511292.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29738..30574)
                     /gene="rhaS"
                     /locus_tag="ACHKW5_001886"
     CDS             complement(29738..30574)
                     /gene="rhaS"
                     /locus_tag="ACHKW5_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO7511293.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30862..32331
                     /gene="rhaB"
                     /locus_tag="ACHKW5_001887"
     CDS             30862..32331
                     /gene="rhaB"
                     /locus_tag="ACHKW5_001887"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO7511294.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYISYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            32328..33587
                     /gene="rhaA"
                     /locus_tag="ACHKW5_001888"
     CDS             32328..33587
                     /gene="rhaA"
                     /locus_tag="ACHKW5_001888"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO7511295.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33829..34653
                     /gene="rhaD"
                     /locus_tag="ACHKW5_001889"
     CDS             33829..34653
                     /gene="rhaD"
                     /locus_tag="ACHKW5_001889"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO7511296.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34663..34977
                     /gene="rhaM"
                     /locus_tag="ACHKW5_001890"
     CDS             34663..34977
                     /gene="rhaM"
                     /locus_tag="ACHKW5_001890"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO7511297.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35278..35724
                     /gene="frvA"
                     /locus_tag="ACHKW5_001891"
     CDS             35278..35724
                     /gene="frvA"
                     /locus_tag="ACHKW5_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO7511298.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            35735..37186
                     /gene="frvB"
                     /locus_tag="ACHKW5_001892"
     CDS             35735..37186
                     /gene="frvB"
                     /locus_tag="ACHKW5_001892"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO7511299.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37176..38246
                     /gene="frvX"
                     /locus_tag="ACHKW5_001893"
     CDS             37176..38246
                     /gene="frvX"
                     /locus_tag="ACHKW5_001893"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7511300.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            38246..39994
                     /gene="frvR"
                     /locus_tag="ACHKW5_001894"
     CDS             38246..39994
                     /gene="frvR"
                     /locus_tag="ACHKW5_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO7511301.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(40044..>41070)
                     /gene="yiiG"
                     /locus_tag="ACHKW5_001895"
     CDS             complement(40044..>41070)
                     /gene="yiiG"
                     /locus_tag="ACHKW5_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO7511302.1"
                     /translation="LSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVA
                     KAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTG
                     EERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMD
                     KYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEE
                     REGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNF
                     SFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMV
                     DDYNSLR"
BASE COUNT         9917 a        10712 c        10672 g         9769 t
ORIGIN      
        1 ggcaggtaaa ttctccgggt attccgggtc gtgcgtcagc cacacggcag acagacggat
       61 gccgttgtcc cggccatatt cggtgtaacc cggcagggta tcaggaaaag cggacggttc
      121 cgtcccgccg gaaatggcct gctgccgggc ttcttctgcc cgctgcgcct gcgtggtcag
      181 taccagccgg aagtgacgcc cggcaccatc cgtcacgccg gtgatttcgc cgctgaattc
      241 accggcggct tcgcggtgga acgtctgtgt gcgcccgaag cggtccacca gcccggtcag
      301 tacccggtac ggcggcagcg gcgcaggcag cacctcatcc gcttccggca cccgctcaca
      361 ccagccgagc agccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact
      421 taagcggagt tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag
      481 tttcgccacg ccgccgcgca ccagccacag tgactcgctg cggctgtaac cgtcctcacc
      541 gggaaacagg tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt
      601 atcgcgcagc tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac
      661 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
      721 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca gaggattgac
      781 cggatggccg gacgtcactc cgccggggca caccgaacag gccacgccgg tgggggcacc
      841 aatgcgcacc ccggctgaac cctggacaat gctaccgcca tactgcgtca tgtcgccctg
      901 acgcgccgcc ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc
      961 ctagcgccag tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt
     1021 tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg
     1081 gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt
     1141 agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt
     1201 caggtcatga ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt
     1261 aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc
     1321 gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg
     1381 atcatttcgt acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc
     1441 gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat
     1501 gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc
     1561 agaaggcatg acggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga
     1621 gcgcatcggg gttgtggtgt cagttagcga tgtcagtgag ctgccgctta acgagctgaa
     1681 agcggtagtg gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcggttact
     1741 gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc
     1801 gattttactg cgccaggggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac
     1861 tgaacaaggc caggcggtgg atctgaacag cctgaaacgc tcccccaagc aacaacaggc
     1921 gctggcggcg ttacggcaag gcaaaatctg gcgcgaccag gtcgccacgc tcgaatttaa
     1981 tgatgccgcg ttgcaggcgc tacgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc
     2041 agagtttagc gactggcaaa cgaactatgc cgtttctggt gagcggttgc gattgaatac
     2101 cgaacaggcc accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct
     2161 gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt
     2221 gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac
     2281 tatcgcccgt tttcgtgaac gttttaatgc ccccgtggaa gttctgcatt ccggcctgaa
     2341 cgacagcgag cggctttcgg cgtggttgaa agcgaaaaat ggcgaggcgg cgattgtgat
     2401 cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgatga
     2461 agagcacgac agctcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc
     2521 ggtgtatcgt gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct
     2581 ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc
     2641 ggggaatgcg cgtccggcaa ttcaacacgt gctggattta aaaggtcaga aggtgcaggc
     2701 aggtctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt
     2761 tatcctcttt cttaatcgtc ggggttttgc gcctgcgcta ctgtgccacg actgcggctg
     2821 gattgccgaa tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt
     2881 gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc
     2941 cacgcacctg gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct
     3001 gttccctgat gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct
     3061 ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat
     3121 gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg
     3181 cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt
     3241 cgctggtcgt gccgggcgtg cgggtaaaca aggcgaagtg gtgctgcaaa cgcaccatcc
     3301 agaacatcct ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc
     3361 gctggctgag cgacggatga tgcaactgcc gccatggacc agccatgtga ttgtgcgtgc
     3421 ggaagatcat aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct
     3481 ctccagcccg ctggcagacg acaaactgtg ggttctcggt ccggttccgg cactggcacc
     3541 taaacgtggc ggtcgctggc gctggcagat attgttgcag cacccttccc gcgtgcgctt
     3601 gcaacacatc attagtggta cgctggcgct catcaataca ataccggatt cccgcaaggt
     3661 gaaatgggta ctggatgttg atccgattga gggttaaacc gcttacgatg cgaggcggat
     3721 cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta
     3781 tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg
     3841 cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc
     3901 ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc
     3961 tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag
     4021 cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat
     4081 atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac
     4141 ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc
     4201 gatttgatca tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt
     4261 gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca
     4321 ccggaactgg agctacctac agttcatatc gacaatctga ccgccgcatt tgatgcagta
     4381 aattatctat atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg
     4441 ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg
     4501 gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcgatg
     4561 cagcagctgc ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg
     4621 gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc
     4681 ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc
     4741 gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag
     4801 gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca
     4861 acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg
     4921 cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg
     4981 agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa
     5041 aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgttct
     5101 tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac
     5161 gctgcaaagc cagaaagtga ccggtaacgg actaccacca aaaccagaag aacgctggcg
     5221 ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag agccttctgc
     5281 cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca
     5341 aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaagttccgt ggaatgaaca
     5401 gacgccggaa cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga
     5461 gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg
     5521 tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca
     5581 gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca
     5641 gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga
     5701 cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagatacggt
     5761 acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa
     5821 tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt
     5881 gaagatggcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa
     5941 tcccccccat ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat
     6001 tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg
     6061 gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa
     6121 aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat
     6181 gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc
     6241 aaagccgccg ttgagctggc gaaagactgg cgtaccgatc gcatgctgcg caaacttgaa
     6301 gcactgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg
     6361 gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg
     6421 gcgcgtgcac tgttagaaaa tactgaactg agtgctcgtg aaattgctga aaaggcgttg
     6481 gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc
     6541 tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg
     6601 gataagcaca tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac
     6661 cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc
     6721 ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg
     6781 gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctacgtcggt
     6841 aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc
     6901 caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac
     6961 gtgctgatcc cacctgctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc
     7021 gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc
     7081 gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa
     7141 gagatgacca gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg
     7201 cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg
     7261 gtgaacccgg aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg
     7321 tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct
     7381 cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa
     7441 cacgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg
     7501 aaaccgtctg acctgatccc ggaactgcaa ggtcgtctgc caatccgcgt tgaactgcag
     7561 gcgctgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg
     7621 cagtacaaag cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt
     7681 aagcgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt
     7741 cgtttacaca ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta
     7801 agcggtcaaa ctatcatcat tgacgcagat tatgtgagca aacatctgga tgcgttggtg
     7861 gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt
     7921 tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc
     7981 cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca
     8041 attatcgtcg ggacagcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg
     8101 ctggcactga ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc
     8161 gatgcggtaa aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa
     8221 ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt
     8281 ctctccgggc tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc
     8341 ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct
     8401 tatggttata tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagtgtc
     8461 atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc
     8521 gcatgcggcc tgctggcaac ggcagtactg aatattaata acctgcgtga tatcaatagc
     8581 gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt
     8641 tatcatgcct gcctgctgat gggctcgctg gtgtgtctgg cgctgtttaa tctcttttcg
     8701 ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc
     8761 cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc
     8821 aagggagcgt tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca
     8881 gcataactga caaatatcaa ttaacaattg atgattttgc caacagccca catagcgcga
     8941 tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc
     9001 tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg
     9061 gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc
     9121 tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt
     9181 ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg
     9241 aaggtctggt catttacggc gcggtgcgtc aggtagatga cctggaagag ttggatatcg
     9301 gcatccaggc gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg
     9361 atgtccgcgt caattttggc ggtgtcacct tcttctccgg cgaccatctt tatgccgaca
     9421 ataccgggat tattctttct gaagatccgc tggatattga atgatagaaa aggcaccgca
     9481 aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac gcggaagatg
     9541 aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg
     9601 ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca
     9661 ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc
     9721 aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct
     9781 aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa tcctcgtccc
     9841 gattaccggt gacgccttaa taaatacgag cgcactttag ttagctccga ttgtatgaag
     9901 ccgcgccatc gctgtccagc ggcacgcctt gcagattacg gtttgccaca cttttcatcc
     9961 ttctcctggt gacataatcc acatcaatcg aaaatgttaa taaatttgtt gcgcgaatga
    10021 tctaacaaac atgcatcatg tacaatcaga tggaataaat ggcgcgataa cgctcatttt
    10081 atgacgaagc acacacattt taagttcgat atttctcgtt tttgctcgtt aacgataagt
    10141 ttacagcatg cctacaagca tcgtggaggt ccgtgacttt cacgcataca acaaacatta
    10201 actcttcagg atccgattat gagtcaaaca tcaaccttga aaggccagtg cattgctgaa
    10261 ttcctcggta ccgggttgtt gattttcttc ggtgtgggtt gcgttgcagc actaaaagtc
    10321 gctggtgcgt cttttggtca gtgggaaatc agtgtcattt ggggactggg ggtggcaatg
    10381 gccatctacc tgaccgcagg ggtttccggc gcgcatctta atcccgctgt taccattgca
    10441 ttgtggctgt ttgcctgttt cgacaagcgc aaagttattc cttttatcgt ttcacaagtt
    10501 gccggcgctg tctgtgctgc ggctttagtt tacgggcttt actacaattt atttttcgac
    10561 ttcgagcaga ctcatcacat tgttcgcggc agcgttgaaa gtgttgatct ggctggcact
    10621 ttctctactt accctaatcc tcatatcaat tttgtgcagg ctttcgcagt tgagatggtg
    10681 attaccgcta ttctgatggg gctgatcctg gcgttaacgg acgatggcaa cggtgtacca
    10741 cgcggccctt tggctccctt gctgattggt ctactgattg cggtcattgg cgcatctatg
    10801 ggcccattga caggttttgc catgaaccca gcgcgtgact tcggtccgaa agtctttgcc
    10861 tggctggcgg gctggggcaa tgtcgccttt accggcggca gagacattcc ttacttcctg
    10921 gtgccgcttt tcggccctat cgttggcgcg attgtaggtg catttgccta ccgcaaactg
    10981 attggtcgcc atttgccttg cgatatctgt gttgtggaag aaaaggaaac cacaactcct
    11041 tcagaacaaa aagcttcgct gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa
    11101 atatatcgtt gcgctcgacc agggcaccac cagctcccgc gcggtcgtaa tggatcacga
    11161 tgccaatatc attagcgtgt cgcagcgcga atttgagcaa atctacccaa aaccaggttg
    11221 ggtagaacac gacccaatgg aaatctgggc cacccaaagc tccacgctgg tagaagtgct
    11281 ggcgaaagcc gatatcagtt ccgatcaaat tgcagctatc ggtattacga accagcgtga
    11341 aaccactatt gtctgggaaa aagaaaccgg caagcctatc tataacgcca ttgtctggca
    11401 gtgccgtcgt accgcagaaa tctgcgagca tttaaaacgt gacggtttag aagattatat
    11461 ccgcagcaat accggtctgg tgattgaccc gtacttttct ggcaccaaag tgaagtggat
    11521 cctcgaccat gtggaaggct ctcgcgagcg tgcacgtcgt ggtgaattgc tgtttggtac
    11581 ggttgatacg tggcttatct ggaaaatgac tcagggccgt gtccatgtga ccgattacac
    11641 caacgcctct cgtaccatgt tgttcaacat ccataccctg gactgggacg acaaaatgct
    11701 ggaagtgctg gatattccgc gcgagatgct gccagaagtg cgtcgttctt ccgaagtata
    11761 cggtcagact aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg
    11821 tgaccagcag gccgcgctgt ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac
    11881 ctatggcact ggctgcttta tgctgatgaa cactggcgag aaagcggtga aatcagaaaa
    11941 cggcctgctg accaccatcg cctgcggccc gactggcgaa gtgaactatg cgttggaagg
    12001 tgcggtgttt atggcaggcg catccattca gtggctgcgc gatgagatga agttgattaa
    12061 cgacgcctac gattccgaat atttcgccac caaagtgcaa aacaccaatg gtgtgtatgt
    12121 ggttccggca tttaccgggc tgggtgcgcc gtactgggac ccgtatgcgc gcggggcgat
    12181 tttcggtctg actcgtgggg tgaacgctaa ccacattata cgcgcgacgc tggagtctat
    12241 tgcttatcag acgcgtgacg tgctggaagc gatgcaggcc gactctggta tccgtctgca
    12301 cgccctgcgc gtggatggtg gcgcagtagc aaacaatttc ctgatgcagt tccagtccga
    12361 tattctcggc acccgcgttg agcgcccgga agttcgcgaa gtgactgcat tgggtgcggc
    12421 ctatcttgct ggtctggcgg tcggtttctg gcagaacctc gacgagctgc aagagaaagc
    12481 ggtgattgaa cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt accgttacgc
    12541 aggctggaaa aaagcggtca aacgcgcgat ggcgtgggaa gaacacgacg agtaatgtaa
    12601 atgccggatg aagcgtttat gccgcatccg gtagtcctga aaccgtgcgg gggcaacccc
    12661 gcacacatca ataatcccac ccttcccctg tgctacactt cgcgccattc cttactgctt
    12721 agagtttgct atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatctgcggc
    12781 gctggctggc tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt
    12841 aaacgccatg cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga
    12901 aggtgaaatc gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg
    12961 cgacgcggta gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca
    13021 ggctaacgcg ctggcggtgc tggcagtagg cgataaaggc tgcttcctca atgcgccgga
    13081 tatgtatatg gagaagctga ttgtcgggcc gggagccaaa ggcaccattg atctgaacct
    13141 gccgctggcg gataacctgc gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact
    13201 gacggtaacg attctagcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact
    13261 cggcgtacgc gtatttgcta ttccggatgg cgacgttgcg gcctcaattc tcacctgtat
    13321 gccagacagc gaagttgacg tgctgtacgg tattggtggc gcgccggaag gcgtagtttc
    13381 tgcggcggtg atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga
    13441 cgtcaaaggc gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa
    13501 agcgatgggc atcgaagccg gtaaagtatt gcgcctgggc gatatggcgc gcagcgataa
    13561 cgtcatcttc tctgccaccg gtattaccaa aggcgatctg ctggaaggca ttagccgcaa
    13621 aggcaatatc gcgactaccg aaacgctgct gatccgcggc aagtcacgca ccattcgccg
    13681 cattcagtcc atccactatc tggatcgcaa agacccggaa atgcaggtgc acatcctctg
    13741 attgatttga tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa
    13801 cgaagataag gcaagttaat caaaacagga gaaaaacatg gctgattggg taacaggcaa
    13861 agtcactaaa gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt
    13921 gcttccgttt accgccgggc aattcaccaa gcttggcctt gaaatcgacg gcgaacgcgt
    13981 ccagcgcgcc tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt
    14041 caccgtcccc gatggcaaat taagcccacg attggcggca ctgaaaccag gcgatgaagt
    14101 gcaggtggtt agcgaagcag ctggcttctt tgttctggat gaagtaccgg actgcgaaac
    14161 gctatggatg ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg
    14221 caaagattta gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga
    14281 cttaagctat ttgccactga tgcaagaact ggaaaaacgc tacgaaggga aactgcgcat
    14341 tcagacggtg gtcagtcggg aaacggcagc ggggtcgctc accgggcgga taccggcgtt
    14401 aattgaaagt ggggaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca
    14461 tgtgatgctg tgcggcaatc cacagatggt gcgcgataca caacagttgc tgaaagagac
    14521 ccggcagatg acgaaacatt tacgtcgccg accgggccat atgacagcgg agcattactg
    14581 gtaagcggtt acttatcgat aaacggcacg atgagcaaat ccgcactcat cttattgatc
    14641 atcccgcgat atgccggcat caaacggttg ataaatgagt gatgatgacc acagacaagg
    14701 aggtcgcact gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca
    14761 atacgcagtt ttgtcttcgg ccattgaata tttttcgtca gtttatacag cttgttatcc
    14821 gacttattct tcaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac
    14881 aactcgctta agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg
    14941 agctccagag ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca
    15001 atgtgtttat aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca
    15061 gtgataactc aaaaataatc atctcggcct ggcatgagaa agtgaaggcc gcatcaagct
    15121 caacttgccc ctccagatcc ttaatcttgt aggccacgct ggccccttca tctgcagagg
    15181 atgcagcggc ggcaagctct ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg
    15241 caaaaaagcg cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa
    15301 tcgcacatcc tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat
    15361 tcgcctctct gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt
    15421 aatgatctta tggattacca ttttttttga tattgcttaa ggtgaagagt tcagaaggat
    15481 tttccgggca ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag
    15541 agatatatta aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgggta
    15601 tctttaccat atttattttc gccttgtgtg ccaatgaacg ctccaagatc gataagcatc
    15661 atcaccagaa tcaacgtcgg gacaaaacgc cccaccgccc attgccagac acccggtaaa
    15721 atcgcccagt tacccgccag cagcatccac gccacaatca tcagaaatgc ccatgcgccg
    15781 gaacgcccgc gatcatgcaa gcgcttaacg gttactgccg ctgttggcca aagcaagcac
    15841 acaaggcaaa acgccgcggt ctgaatatcg agtaaatttt tacccgccag tgagaaaagc
    15901 accagcatgc ctgcgaacca caggcctatc caaatccaga aatcacggcg tccaatacgc
    15961 cctttaaatg agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt
    16021 ccaggattct acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa
    16081 caaaaggtac tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg ttctgcatta
    16141 accgttacaa caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg
    16201 ctggctggag cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc
    16261 cagaatccca gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc
    16321 aatctcactc gtgctgcaag taaaattaac gagaacttgt atgcttctac agcgctggag
    16381 cgcggtacct taaaaatcaa aagcattcaa atgacctggc tacccatcca ggggccagag
    16441 caaaaagcag cgaaagcgaa agctcaggaa tacatggcag cagtgatccg cacactcacc
    16501 ccattaatga ccaaaacaca aagccagaaa aaactgcagt cgctgctaac ggcggggaaa
    16561 aacaaacgtt attacaccga gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc
    16621 gaaaaggggc tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa
    16681 ggtttgaata aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac
    16741 agacctgctg ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta
    16801 aaatgcgaca tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc
    16861 acgagctggt ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa
    16921 tcgcaccacc ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc agccacatca
    16981 tgctgggtgc gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacttctg
    17041 ccgctatgct gaaagacatc ggcgctcagt acatcatcat cggccactct gaacgtcgta
    17101 cttaccacaa agagtctgac gaactgatcg cgaaaaaatt cgcggtgctg aaagagcagg
    17161 gcctgactcc ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggcaaaactg
    17221 aagaagtttg cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg
    17281 aaggtgcggt tatcgcttac gaacctgtat gggcaatcgg tactggcaaa tctgcaactc
    17341 cggctcaggc acaggctgtt cacaaattca tccgtgacca catcgctaaa gttgacgcta
    17401 acatcgctga acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag
    17461 aactgtttgc tcagccagat atcgacggcg cgctggttgg cggtgcttct ctgaaagctg
    17521 acgctttcgc agtaatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg
    17581 tgccggattt catatccggc acttactttc cttaactctt cgccttaacg caaaatctca
    17641 cactgatgat cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag atttcgttgt
    17701 gtcgccagca atacaaatga gttatcactc tgccgtacca tcgccagccc gtagcttccc
    17761 atatgttccc gcgcctcagg tacttcttct gccagcatca taaatgggct gcgttgtacc
    17821 agttcgcttt ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt
    17881 ggcaaccagc ggctgctgat gttcgccaga ttgttatcca gttgttcgcg cacatcagga
    17941 cgaatacaag agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc
    18001 gccaaagaaa ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc
    18061 gcctgccagg ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca
    18121 gtaccgttaa tacgatacgt tggcatcaac agatattgca gtggaccatt aagatctttt
    18181 aaaaccacgt atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc
    18241 tgctgattgg gcaaacattc ctcaaggaca atcttacgta atgtatccga ctcttcaccg
    18301 gttaatttcc agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa
    18361 agaagacccg cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc
    18421 aaaatcgtga caaataaaaa acccggtcac gagaaaccac cgggtttgat aattatcctg
    18481 gggaaatcag cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg
    18541 cgctttcgtc cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc
    18601 attttcgtac tttttcgcca cctcagcgtc gcgcggacgg tagtagtttt tcgcggcaat
    18661 ttcctgacct tctggcgagt agagatattt caggtaggct tccgccacct ctttggtgcc
    18721 ttttttctcg accactttat cgaccaccga cacggttggc tctgcgagga tagactcact
    18781 cggcgtgacg atttcgaatt tatctttccc cagttcattc gctgccagca gagcttcgtt
    18841 ttcccaggcg atcagtacat cgccaatccc gcgctcgaca aaggtgttag tggagccgcg
    18901 cgcgccagaa tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt
    18961 tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag gcagccaggt agttccagcg
    19021 tgcgccacca gagcttttcg gattaggcgt gatcaccgaa acacccggtt taatcagatc
    19081 gttccagtca tggatctgct tcggattgcc cttacgcacc aggaaaacaa tggtggaagt
    19141 gtacggtgcg gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc
    19201 cgcaattgcg tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt
    19261 gattaccgac gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca ccacgttatc
    19321 accagtttgc tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg
    19381 cgttggatca tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc
    19441 cagcaaaaat gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt
    19501 gtgatgagca aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct
    19561 ataacttgtg ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc tcatattgct
    19621 gacaaagtgc gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag
    19681 tttgcaaatt caataaattg cagaattcat gtaggcctga taagcgaagc gcatcaggca
    19741 tttttgcttc tgtcatcggt ttcagggtaa aggaatctgc ctttttccga aatcattaat
    19801 acagtttttt cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg ttttcgatag
    19861 cgtcgatgat gtcgtggtga accagctgtt cgttctggat acctacacaa cgaccgccgt
    19921 aacctgccag cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt
    19981 aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac
    20041 cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg
    20101 ccacgatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt accaggtctt
    20161 cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca gccaccggca atggccgcag
    20221 ccaacgtcag atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag
    20281 aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga
    20341 aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc agaccgatgc
    20401 acgggaagcc catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa
    20461 ccaccagcgc gtcgatccca cgttttttca ggttttcgat agccacggcg cggatgttct
    20521 cgtcgcggaa ttccgggaag cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt
    20581 cagaaacgct gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt
    20641 cataaatgcc cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg
    20701 cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc ttaatcatga
    20761 ctacctctga actttggaat gcaaaataaa atctgttgcc ggaagtcttc ttgcacatcg
    20821 aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt
    20881 caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt
    20941 atgaaagcat agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa
    21001 ttacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat
    21061 gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata aagcgggtcg
    21121 gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga
    21181 tatcaataat ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat gactgtaccg
    21241 cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca
    21301 gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga attgcgccgt
    21361 tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg ctttgcgtcc
    21421 ggcgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc gccacaattg
    21481 tcacgataac cccgacgcct ggatctgtca tcggtgttgg agatatcaga tgttgaatac
    21541 ccgtcaaaaa caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga
    21601 gggactctgc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc
    21661 gcaccaccag taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg
    21721 cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcaacg
    21781 aagccatcgc cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca
    21841 taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt
    21901 aagcagcagg caaattgagg ataaaaaaaa cccccacatc atgtggggga agacagggat
    21961 ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct actactcaat
    22021 agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc cattcgttgt
    22081 tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta acaggcgata catttggttg
    22141 cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt ttctgcctgt
    22201 gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat tgtctccagt
    22261 tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc tcgcatctgc
    22321 tgacgctgat gttcggttaa acttatgccg tcaaacatat ggctctgcgt actgcgctgc
    22381 gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc gtggcttaat
    22441 gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat catttgctcc
    22501 caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg caaacatgcg
    22561 tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac gtaatttctg
    22621 cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc gagagctgac
    22681 ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg cccacgatgg
    22741 ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg acgtaatgat
    22801 gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc agacgcctgt
    22861 cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg agctgggcgc
    22921 agatgactat ctcccgaaac catttaatga tcgtgagctg gtggcacgta ttcgcgcgat
    22981 cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt caccgacact
    23041 ggaagttgat gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg acgggcaaac
    23101 gctggagtta accggcactg agtttaccct gctctatttg ctggcacagc atctgggtca
    23161 ggtggtttcc cgtgaacatt taagccagga agtgctgggc aaacgcctga cgcctttcga
    23221 ccgcgctatc gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc gtaaagatgg
    23281 tcacccgtgg tttaaaacct tgcgtggtcg cggctatctg atggtttctg cttcatgata
    23341 ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct ggtgttgatg
    23401 ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct ggatagcgaa
    23461 cagcgtcagg ggctgatgat tgagcagcat gttgaagcgg aactggcgaa cgatccgccc
    23521 aacgatttaa tgtggtggcg acgtctgttc cgggcgattg ataagtgggc accgccagga
    23581 cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg cagcgaaatg
    23641 cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca gaagaaaaag
    23701 tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga taattaccaa
    23761 ctttatctga ttcgtccggc cagcagttct caatccgatt tcattaactt actgtttgac
    23821 cgcccgctat tactgctgat tgtcaccatg ttggtcagta cgccgctgct gttgtggttg
    23881 gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga agttgcccag
    23941 ggaaacttac gccagcaccc ggaacttgaa gcggggccac aggaattcct tgccgctggt
    24001 gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca gcagcgtctg
    24061 ctttctgata tctctcacga gctgcgcacc ccgctgacgc gtctgcaact gggtacggcg
    24121 ttactgcgcc gtcgtagtgg tgaaagcaag gaactggagc gtattgaaac cgaagcgcaa
    24181 cgtctggaca gcatgatcaa cgatctgttg gtgatgtccc gtaatcagca aaaaaacgcg
    24241 ctggttagcg aaaccatcaa agccaaccag ttgtggagtg aagtgctgga taacgcggcg
    24301 ttcgaagccg agcagatggg caagtcgttg acggttaact tcccgcctgg gccgtggccg
    24361 ctgtacggca atccgaacgc cctggaaagt gcgctggaaa atattgttcg taatgctctg
    24421 cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg tatcaccatt
    24481 acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat tttccgtccg
    24541 ttctatcgga ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt ggggctggcg
    24601 attgttgaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga cagcccgctg
    24661 ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac tccgcatttg
    24721 taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct gatgcgacgc
    24781 tgatagcgtc ttatcaggcc tacactccgg gggttatttt ccccacaacc ggcgggaaaa
    24841 atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa
    24901 cccggcggcg gagagtaaac ggtgatattg atcgtcatca aacggcatat gccatgcgat
    24961 ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca gcggcgcgtc
    25021 cgcagacact ttccctggcg ctatcacgct atacaaccac cccaccttac ctgtgttttg
    25081 catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc aaggcgagcg
    25141 cggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccca tataaacatt
    25201 actttccgtc aggccgtcgg ttgagaggtt ttcaccaaac gcaggcgcaa caaacaactc
    25261 cgcctgttcc ggaaattccc gcgcccagta gagataatgc tcacgaggat aatgacacag
    25321 cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt ccagccccag
    25381 ctccgtcagc atcaactcac catcaacctg gattttggca attgcgctgg gtttgccttc
    25441 gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct gttccctctc
    25501 cacgagaaat ttccccatta gaccatattt attttatgtc aggcgtgctg ttttcgcggt
    25561 aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc cggctgtggg
    25621 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
    25681 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
    25741 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
    25801 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
    25861 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
    25921 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
    25981 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
    26041 attctcgtaa ttcagggtca aggttcccca gggcaaagcc caccaggaac ggtaatactg
    26101 cgccgacgaa aacatgcggt tcaaacgagg caatccctgc agtgcccaga ataatcatcg
    26161 tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct tcttttgtgc
    26221 cgtactgctg catgatggaa gcgtaaagtc cgccattggt catatccatc gccgccacca
    26281 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
    26341 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt tgttaccacc agcgtaccgg
    26401 atttacgcaa taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
    26461 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
    26521 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
    26581 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
    26641 tttttaaggg ctgatgcgtc attcctcaat ggagcatcag tgttgatgga aacgaagata
    26701 gacattcatt taatcaataa aagtgatcaa aaccacatca attgaaacac tgttttattt
    26761 ttatcggatc attgttttta ttttgaaatt gatcacaaaa aaaccaccgc cgttggcgat
    26821 ggttcatttt ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaatgat
    26881 tattttttcg ccgcgaaacg cgccgcagct tcgtcccagt tcaccacgtt ccagaactct
    26941 ttgatgtagt ccgggcggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc
    27001 aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc
    27061 tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg
    27121 gaaccaaagc gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg
    27181 aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct
    27241 ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg
    27301 tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct
    27361 ggcaggcttt ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg
    27421 tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg
    27481 gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc
    27541 gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg
    27601 tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca
    27661 cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac
    27721 agcacgattt ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa
    27781 gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc
    27841 agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc
    27901 agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc
    27961 ggatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt
    28021 cggcgctatg tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcct
    28081 gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc
    28141 aattatcaac ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttgct
    28201 cggcgttctg gtggcgctga ttggcgtagg gattgtgact cgcgccgggc agttgaaaga
    28261 gcgcaaaatg ggcattaaag ccgaagagtt caacctgaaa aaagggctgg tgctggcggt
    28321 gatgtgcggc attttctctg ccggtatgtc ttttgcgatg aacgccgcaa aaccgatgca
    28381 tgaagccgct gccgcactgg gcgtcgatcc actgtatgtc gctctgccaa gctatgtcat
    28441 catcatgggc ggcggcgcga tcatcaacct cggtttctgc ttcattcgtc tggcaaaagt
    28501 gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt
    28561 gttactctca gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctgggg
    28621 ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta
    28681 tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaatg caggtcgccg
    28741 tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcca acatcgtcgg
    28801 catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat
    28861 cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt
    28921 cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct
    28981 gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa
    29041 aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag
    29101 gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa
    29161 cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc
    29221 agccatttcg ttagcaaacg acacatgctg actactttca tgctcaagct gaccgataac
    29281 ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc
    29341 gctaaatccc ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata
    29401 aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg
    29461 aatgtaaaag agatcgccac gggtaatgcg ataagggcga tcgttgagta catgcagacc
    29521 attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaaaacatc
    29581 ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt
    29641 gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt
    29701 cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaaa ccatcccgtc
    29761 cctgacgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt
    29821 tactgtcgct gaatccacag cgataggcga tatcagtaac gctggcctcg ctgtggcgta
    29881 gtagatgtcg ggctttcatc agtcgcaggc ggttcaggta acgctgaggc gtcagtcccg
    29941 tttgctgctt aagctgccga tgtagcgtac gcagcgaaag agaaaattgc tccgccacgg
    30001 catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac
    30061 gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt
    30121 gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt
    30181 ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtggg
    30241 aaggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa
    30301 atcgatccgg cgagcgatac agcacattag tcagacacag attatcggta tgttcataca
    30361 gatgccgatc atggtcgcgc acaaaacaga ccgtgccacc ggtgatggta tagggctgcc
    30421 cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat
    30481 gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttaccag
    30541 acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg
    30601 gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa
    30661 aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc
    30721 accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
    30781 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
    30841 gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca
    30901 tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc
    30961 gaaatccatc gttttaacaa tgggctgcat agtcagaacg gctatgtcac ctgggatgtg
    31021 gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt
    31081 atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt
    31141 cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatggcgcag
    31201 gcacaacaac aactcggcaa acgcgatatt tatcaacgta gcggcatcca gtttctgccc
    31261 ttcaatacgc tttatcagtt acgggcgctg acggagcaac aacctgaact tattccacac
    31321 attgctcacg ctctgctaat gccggattat attagttatc gccttaccgg caagatgaac
    31381 tgggagtaca ccaacgccac aaccacgcaa ctggtcaata tcaatagcga cgactgggac
    31441 gagtcgctac tggcgtggag cggtgccaac aaagcctggt ttggtcgccc gacgcatccg
    31501 ggtaatgtca taggtcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt
    31561 gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct
    31621 tatctctctt ctggtacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat
    31681 gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg
    31741 ctgaaaaata ttatgggctt atggctgctt cagcgggtgc ttcaggaacg gcaaatcaac
    31801 gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgtcgctt catcatcaat
    31861 cccaatgacg atcgctttat taatcctgaa gcgatgtgca gcgaaattca ggctgcgtgt
    31921 cgggaaacgg cgcaaccgat cccggaaagt gatgctgaac tggcgcgctg tattttcgac
    31981 agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat
    32041 ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc
    32101 gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat
    32161 atcggcatcc agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc
    32221 gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac
    32281 tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac
    32341 tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg
    32401 aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg
    32461 tttccggttt tgaaaacccg gaaggttcgc tgaccggtgg gattcaagct actggtaatt
    32521 atccgggcaa agcgcgtaat gccagtgagc tacgtaccga tctggaacag gctatgcggc
    32581 tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag
    32641 tctcgcgcga tcagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca
    32701 atcagctcgg tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct
    32761 ttacgctttc ccatgccgac gacagcattc gccagttctg gattgatcac tgcaaagcca
    32821 gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct
    32881 ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc
    32941 tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg
    33001 ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt
    33061 acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc
    33121 cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtgccg cagttgctgc
    33181 tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa
    33241 cccaggcaat tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc
    33301 ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata
    33361 tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag
    33421 cggcgggcga ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgtggc
    33481 aggcggtctg ggaaatgtat tgccaacgtc acgatacgcc agcaggtagc gaatggctgg
    33541 agagcgtgcg ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg
    33601 cggcgcgagc gccttatccg gcctacaggt cgaatgtcca tgcgggctgt caccctcacc
    33661 ccggccctct ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg
    33721 aacactgctc cggacagtcc cctcgcccct acggggagag ggttagggtg aagggaacaa
    33781 gcagcaccat gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt
    33841 acccagtcct ggtttgtcca gggaatgatc aaagccacca ccgatgcctg gctgaaaggc
    33901 tgggacgagc gcaacggcgg taacctgacg ctgcgcctgg atgacgccga tatcgcgcct
    33961 tataaagaca acttccatgc acaaccgcgc tatatcccgc tcagccaacc catgccttta
    34021 ctggcaaata caccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctt
    34081 gatcctgcag ctaacttagg cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt
    34141 ctctgggggt taaccaacga agccgttcca acttctgaac ttccggctca ctttctctcc
    34201 cactgcgagc gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc
    34261 accaacctga tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa
    34321 ctgtgggaag gcagcaccga gtgtctggta gtgttcccgg atggcgttgg cattttgccg
    34381 tggatggtgc ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat
    34441 tcgctggtgt tgtggccctt ccacggcgtc ttcggcagcg ggtcgacgtt ggatgaaaca
    34501 ttcggtttaa tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg
    34561 ggcggcatga aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc
    34621 gttacaccac tagctagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt
    34681 gtcatgcagg ttaaccccga cgcccacgaa gagtatcagc gtcggcataa ccccatctgg
    34741 ccggaactgg aagcagtgtt gaaatctcac ggtgcgcata actacgccat ttacctcgac
    34801 aaagcgcgta atctgctgtt tgccacggta gagattgaat ctgaagaacg gtggaatgcg
    34861 gtagccagca ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct
    34921 aacccggata acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg
    34981 cccaatgtgc cagagatgcc tccggcacaa tattttaaat ttcgatttga aaaattaaac
    35041 atattacatc tcccggtaag ttatatttcc ctgatacatt gtgagtaaat cacaaaaata
    35101 atgaataacc cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc
    35161 gcaaatctga cctttcactc tgtctcaaat tgtcattgtt taccgacata tcgcctctca
    35221 tcacttaatc gctccaccat tacaattgat taaaatatat tttatggagt ccgattaatg
    35281 gcagctctta ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccgtt
    35341 ctgaagcagt tggcgacaat agcgttacaa aacggtttta tcaccgactc acaccagttc
    35401 ctgcaaaccc tgctgctgcg cgaaaaaatg cactctactg gatttggttc cggtgtcgcc
    35461 gtgccgcacg gtaaaagcgc ctgcgttaaa caaccgttcg tattattcgc ccgcaaagcg
    35521 caggctattg actggaaagc cagcgatggc gaagacgtca attgctggat ctgcctcggc
    35581 gtgccgcaaa gcggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt
    35641 attcacaagg aatttattca tcaactgcaa cagggcgata ccgaccaggt gcttgccttg
    35701 ttaaatcaaa ccctcagctc ataaggaagt ggcgatggag tcatccttac gtattgtcgc
    35761 gatcaccaac tgccccgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca
    35821 gaaagcccgt tctctcggtc ataccattaa agttgaaact caaggatcaa gcggcgtcga
    35881 aaaccgctta tccagcgaag agattgccgc tgccgattac gtcattctcg ctaccgggcg
    35941 tggcctgagc ggtgacgatc gcgcgcggtt tgccgggaag aaggtttatg agattgccat
    36001 ctcccaggcg ctgaaaaata tcgatcagat tttcagtgaa ttaccaacaa actcgcatct
    36061 ttttgctgca gatagcggcg tgaagcttgg caaacaggaa gtgcaaagcg gcagcgtgat
    36121 gagccacctg atggcgggcg tctctgcggc gctgccgttt gtcatcggcg gcggtattct
    36181 ggtggcgctt gccaacatgc tggtgcagtt cggtttgcca tataccgata tgtcgaaagg
    36241 tgcgccgtcg tttacctggg tagttgaatc catcggttac ctcggcttca cctttatgat
    36301 ccccatcatg ggcgcttata ttgcctcgtc gattgccgat aaaccggctt tcgctccggc
    36361 gtttctggtt tgctatctgg cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg
    36421 attcctcggc gcggtggtgc tggggctggc gattggctat ttcgtcttct ggttccgcaa
    36481 agtgcgcctt ggcaaagcat tacaaccgct gctcggttca atgctgatcc cgttcgttac
    36541 tctgctggtt ttcggcgtgc tcacttacta cgttatcggg ccggtgatgt ccgacctcat
    36601 gggcgggctg ctccacttcc tgaacaccat tcctccatca atgaagtttg cggcagcgtt
    36661 tctggtcggt gcgatgctgg cgttcgatat gggcggcccc atcaataaaa ccgcctggtt
    36721 cttctgcttc tcactgctgg aaaaacacat ttatgactgg tacgccatcg tcggcgttgt
    36781 cgcgttgatg ccgcccgtcg cggcgggtct tgcgacgttc attgcgccca aactgtttac
    36841 ccgacaggaa aaagaggctg ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga
    36901 accggctatt ccttacgctc tggcagctcc gttgccgatg attaccgcca atacgctggc
    36961 gggcggcatt accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cgcctggttt
    37021 gggtatcttt gacccgctga tcggcctgat gtcgccggta ggttcgtttt atctggtgct
    37081 ggcgattggc ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tgtggttacg
    37141 ccgcaaagcg aaagccgcgc aacaggagct tgtccatgaa cattgagtta ctgcaacagt
    37201 tgtgcgaagc cagcgccgtc agcggcgatg aacaggaagt tcgcgacatc ctgataaaca
    37261 cgctggaacc ttgcgttaat gaaatcacct ttgatggtct gggcagcttt gttgcccgta
    37321 aggggaataa aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc ggctttatgg
    37381 tcacccacat cgacgagagc ggttttctgc gttttaccac cattggcggc tggtggaatc
    37441 agtcgatgct caaccaccgg gtaaccatac gcacacacaa gggagtgaaa atccctggtg
    37501 tgattggttc cgtcgcgcct catgcgttaa cggaaaagca aaagcaacaa ccgctgtcat
    37561 ttgatgagat gttcattgat attggcgcga acagtcgcga tgaggtggaa aaacgcggtg
    37621 tggcgatggg cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaag
    37681 tggtcggcaa ggcgctggat aaccgcatcg gctgcgcaat gatggctgaa ctattgcaga
    37741 cggtgaataa tcccgaaatt acgctgtatg gcgtcggcag cgtggaagaa gaagttgggc
    37801 tacgcggggc acaaacctcg gcggaacaca ttaaaccgga tgtagtgatc gtgctggata
    37861 ccgctgtcgc aggcgatgtt ccgggcattg ataacattaa atacccgctg aaactgggcc
    37921 aggggccggg gctgatgctg tttgacaagc gctacttccc caaccagaaa ctggtagcag
    37981 cgttaaaaag ctgtgccgca cataacgatt taccgctgca attttccacc atgaaaaccg
    38041 gtgcgacgga tggcgggcgc tacaacgtga tgggcggcgg gcgtccggtt gttgcgctgt
    38101 gcctgccaac ccgctatctg cacgctaaca gcggtatgat ttcaaaagcc gattacgatg
    38161 ctctgctcac gttgatacgg gattttctga ccaccttaac cgcggagaaa gtcaacgcgt
    38221 ttagccagtt ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga ttgtcgatct
    38281 gctggagcaa cagccgcgca cgcctggtga gctggcgcaa cagactggcg tttcaggcag
    38341 gaccatcctg cgtgatattg actatctcaa ctttaccctt aacggcaaag cccgcatttc
    38401 cgccagtggt agtgcgggct atcagctgga aatcttcgag cgccgcagct tttttcagtt
    38461 gctgcaaaag cacgataacg acgatcggct gctggcactg ttattactga atacctttac
    38521 tccccgtgcg caactcgcct cggcgcttaa tttgccagaa acgtgggtcg cagagcgtct
    38581 gccccggtta aaacagcgtt atgaacgcac ttgttgcctg gccagccgcc ctggtttggg
    38641 ccatttcatt gatgagacag aagagaaacg cgttatcttg ctggcgaact tgctgcgcaa
    38701 agatccgttt ttaattccgc tggcgggcgt aacacgagac aaccttcagc atttatccac
    38761 ggcctgcgac aaccaacacc gctggccgct catgcagggt gattatctct ccagcctgat
    38821 tctggcgatt tacgccctgc gtaatcaact gaccgatgag tggccgcaat atcccggtga
    38881 cgagataaaa caaatcgttg aacatagcgg tctgtttctt ggtgataacg ctgtaagaac
    38941 cctgacaggt ttgatagaga aacagcatca gcaagcgcag ataatttctg ccgataatgt
    39001 gcagcggttg ctgcaaaggg tgccgggcat cgcgtcattg aatattattg atacgcagct
    39061 ggttgagaac attaccgggc atttattacg ttgccttgcc gcaccggtgt ggattgctga
    39121 gcaccgccaa agcagcatga ataacctgaa agccgcctgg cctgcggcct ttgatatgag
    39181 tctgcacttt attacgctac tgcgtgaaca gctcgatatt ccccttttcg acagcgatct
    39241 gatcggtttg tattttgcct gtgcgctgga acggcatcaa aacgaacgcc agccgatcat
    39301 tttgctctcg gaccagaacg cgattgccac tattaatcag ctcgccattg agcgcgatgt
    39361 tttacattgt cgggtgatta ttgcccgtag cttaagcgaa cttgttgcca ttcgcgaaga
    39421 gattgagccg ttattgatca ttaacaacag ccattattta ctggatgacg cggtaaataa
    39481 ttacatcacc gtaaaaaata tcattacggc tgccggtatc gaacaaataa aacattttct
    39541 ggcgacggca tttattcgcc aacagccgga gcgttttttc tctgcccccg gaagttttca
    39601 ttattcgaat gtacgcggtg aaagctggca acatattacc cggcaaattt gtgcgcaatt
    39661 agtggcacaa caccatatta ccgccgatga agcacaacgc atcatcgccc gcgaaggcga
    39721 aggtgaaaac ctgattgtta atcgcctcgc catcccacat tgctggagcg aacaggagcg
    39781 acgttttcgt ggatttttta ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat
    39841 taaccatgtc ttgatcgcct gcgccgccgc cgatgcgcgt catgagctaa aaatatttag
    39901 ctatctggca agcgtattgt gtcagcatcc ggcagaggtt attgccgggt taacaggata
    39961 tgaggcattt atggagttac ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac
    40021 actgcattta tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg
    40081 tactcgcgta atgctctcgg gaaagagtct tcgaccatcc agcttgagtt tgcgtcctga
    40141 agttgctctt tatcccagtc agagtacggg actttatctc tgatgcggcg aacgtatttt
    40201 ttagcctcga gttgatactg gcctgccgaa ttaataaacg agaaattcat gccgccttta
    40261 tccgcttctt tggcctgcgc cactagcgtt tccagttcag acactttctt cattgccgca
    40321 tcaacatcaa acttctcttg cgatatcagg ttattaattt gtttcgctga aatcatgact
    40381 gccagagagt agtagtggaa tgttttccct tctctttctt caatattttt cagttccgca
    40441 agctggcgct tatcgttaat ttcctgaatc gccgcatgat aagattctgc tacaggttca
    40501 aaggcttcca gattttttaa gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg
    40561 tctttataat tttcctgggt gtaatattta tccatttcgt taatggtatt acccaacgcc
    40621 acggcagcat cgatataact caccgccacg ccatcaattg gttgcagcgc aggcgttaac
    40681 gccacagcac tttttacgcc tttttcacac tcagcgaggt tggattcact aatgccgtag
    40741 atgccataaa cagtacgctc ttcaccggta ggcccctgtt taaaatcttt cagccagtca
    40801 gcataacgcg ccagactgct ctgtacaggg atctgtaact tgttatagca cttgatatag
    40861 acatccattt tttcgtcgaa aaccgcttgt tcatcgacca ccggttgcgc aggtgtttca
    40921 ggtttagctt ctgcttttgc aacgggagct tcaggagctg gtgcggcagg ttgactattg
    40981 gcaggcgggg gcgtttctgt ttcggctttt ttatcatcac aacccgtcag accgagggcc
    41041 atgatggcga ctataatagc ggaagataat
//
LOCUS       DAZFYC010000032        40916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFYC010000032 DAZFYC010000000
VERSION     DAZFYC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.032"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(115..2811)
                     /gene="mgtA"
                     /locus_tag="ACHKW5_001896"
     CDS             complement(115..2811)
                     /gene="mgtA"
                     /locus_tag="ACHKW5_001896"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO7511303.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(2952..3005)
                     /gene="mgtL"
                     /locus_tag="ACHKW5_001897"
     CDS             complement(2952..3005)
                     /gene="mgtL"
                     /locus_tag="ACHKW5_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO7511304.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            3190..4137
                     /gene="treR"
                     /locus_tag="ACHKW5_001898"
     CDS             3190..4137
                     /gene="treR"
                     /locus_tag="ACHKW5_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO7511305.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            4256..5677
                     /gene="treB"
                     /locus_tag="ACHKW5_001899"
     CDS             4256..5677
                     /gene="treB"
                     /locus_tag="ACHKW5_001899"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO7511306.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            5727..7382
                     /gene="treC"
                     /locus_tag="ACHKW5_001900"
     CDS             5727..7382
                     /gene="treC"
                     /locus_tag="ACHKW5_001900"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO7511307.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLGYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRG
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            7776..9914
                     /gene="nrdD"
                     /locus_tag="ACHKW5_001901"
     CDS             7776..9914
                     /gene="nrdD"
                     /locus_tag="ACHKW5_001901"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO7511308.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDKWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            10072..10536
                     /gene="nrdG"
                     /locus_tag="ACHKW5_001902"
     CDS             10072..10536
                     /gene="nrdG"
                     /locus_tag="ACHKW5_001902"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO7511309.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(10581..10967)
                     /gene="cybC"
                     /locus_tag="ACHKW5_001903"
     CDS             complement(10581..10967)
                     /gene="cybC"
                     /locus_tag="ACHKW5_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO7511310.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(11150..12502)
                     /gene="pmbA"
                     /locus_tag="ACHKW5_001904"
     CDS             complement(11150..12502)
                     /gene="pmbA"
                     /locus_tag="ACHKW5_001904"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO7511311.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGTLTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            12596..13147
                     /gene="yjgA"
                     /locus_tag="ACHKW5_001905"
     CDS             12596..13147
                     /gene="yjgA"
                     /locus_tag="ACHKW5_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO7511312.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(13230..14603)
                     /gene="mpl"
                     /locus_tag="ACHKW5_001906"
     CDS             complement(13230..14603)
                     /gene="mpl"
                     /locus_tag="ACHKW5_001906"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO7511313.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTIDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPTHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            14779..15777
                     /gene="fbp"
                     /locus_tag="ACHKW5_001907"
     CDS             14779..15777
                     /gene="fbp"
                     /locus_tag="ACHKW5_001907"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO7511314.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(15810..16805)
                     /gene="yjfF"
                     /locus_tag="ACHKW5_001908"
     CDS             complement(15810..16805)
                     /gene="yjfF"
                     /locus_tag="ACHKW5_001908"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO7511315.1"
                     /translation="MIKRKLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(16792..17814)
                     /gene="ytfT"
                     /locus_tag="ACHKW5_001909"
     CDS             complement(16792..17814)
                     /gene="ytfT"
                     /locus_tag="ACHKW5_001909"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO7511316.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(17828..19330)
                     /gene="ytfR"
                     /locus_tag="ACHKW5_001910"
     CDS             complement(17828..19330)
                     /gene="ytfR"
                     /locus_tag="ACHKW5_001910"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO7511317.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFDLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKAQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(19470..20426)
                     /gene="ytfQ"
                     /locus_tag="ACHKW5_001911"
     CDS             complement(19470..20426)
                     /gene="ytfQ"
                     /locus_tag="ACHKW5_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO7511318.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            20736..21266
                     /gene="ppa"
                     /locus_tag="ACHKW5_001912"
     CDS             20736..21266
                     /gene="ppa"
                     /locus_tag="ACHKW5_001912"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO7511319.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(21646..21987)
                     /gene="ytfP"
                     /locus_tag="ACHKW5_001913"
     CDS             complement(21646..21987)
                     /gene="ytfP"
                     /locus_tag="ACHKW5_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO7511320.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(21990..25769)
                     /gene="tamB"
                     /locus_tag="ACHKW5_001914"
     CDS             complement(21990..25769)
                     /gene="tamB"
                     /locus_tag="ACHKW5_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO7511321.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNCTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(25766..27499)
                     /gene="tamA"
                     /locus_tag="ACHKW5_001915"
     CDS             complement(25766..27499)
                     /gene="tamA"
                     /locus_tag="ACHKW5_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO7511322.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            27705..28343
                     /gene="msrA"
                     /locus_tag="ACHKW5_001916"
     CDS             27705..28343
                     /gene="msrA"
                     /locus_tag="ACHKW5_001916"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO7511323.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            28666..30009
                     /gene="paeA"
                     /locus_tag="ACHKW5_001917"
     CDS             28666..30009
                     /gene="paeA"
                     /locus_tag="ACHKW5_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO7511324.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(30071..30277)
                     /gene="ytfK"
                     /locus_tag="ACHKW5_001918"
     CDS             complement(30071..30277)
                     /gene="ytfK"
                     /locus_tag="ACHKW5_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO7511325.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            30602..31159
                     /gene="ytfJ"
                     /locus_tag="ACHKW5_001919"
     CDS             30602..31159
                     /gene="ytfJ"
                     /locus_tag="ACHKW5_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7511326.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(31149..31889)
                     /gene="cysQ"
                     /locus_tag="ACHKW5_001920"
     CDS             complement(31149..31889)
                     /gene="cysQ"
                     /locus_tag="ACHKW5_001920"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO7511327.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            32079..34022
                     /gene="cpdB"
                     /locus_tag="ACHKW5_001921"
     CDS             32079..34022
                     /gene="cpdB"
                     /locus_tag="ACHKW5_001921"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO7511328.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(34151..34531)
                     /gene="ytfH"
                     /locus_tag="ACHKW5_001922"
     CDS             complement(34151..34531)
                     /gene="ytfH"
                     /locus_tag="ACHKW5_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO7511329.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            34620..35480
                     /gene="qorB"
                     /locus_tag="ACHKW5_001923"
     CDS             34620..35480
                     /gene="qorB"
                     /locus_tag="ACHKW5_001923"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7511330.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            35589..36554
                     /gene="ytfF"
                     /locus_tag="ACHKW5_001924"
     CDS             35589..36554
                     /gene="ytfF"
                     /locus_tag="ACHKW5_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7511331.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            36662..37324
                     /gene="ytfE"
                     /locus_tag="ACHKW5_001925"
     CDS             36662..37324
                     /gene="ytfE"
                     /locus_tag="ACHKW5_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO7511332.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(37369..38781)
                     /gene="cycA"
                     /locus_tag="ACHKW5_001926"
                     /gene_synonym="dagA"
     CDS             complement(37369..38781)
                     /gene="cycA"
                     /locus_tag="ACHKW5_001926"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO7511333.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(39090..39710)
                     /gene="fklB"
                     /locus_tag="ACHKW5_001927"
     CDS             complement(39090..39710)
                     /gene="fklB"
                     /locus_tag="ACHKW5_001927"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7511334.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            39929..40567
                     /gene="ytfB"
                     /locus_tag="ACHKW5_001928"
     CDS             39929..40567
                     /gene="ytfB"
                     /locus_tag="ACHKW5_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO7511335.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(40551..>40916)
                     /locus_tag="ACHKW5_001929"
     CDS             complement(40551..>40916)
                     /locus_tag="ACHKW5_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7511336.1"
                     /translation="WDYYIANPWFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMES
                     LLDEGKKHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKV
                     IKETILSWLLVDPSSTAHE"
BASE COUNT         9913 a        11029 c        10340 g         9634 t
ORIGIN      
        1 ttcctgcaat ttattgaatt tgttggccgg ataaggcatt tacgccgcat ccggcatgaa
       61 caaagcttac tttgtctaca atctgaatcg gggctatcgt gcccagttta ttctttattg
      121 ccagccgtaa cgacggctat aaaatccttt caccaactgg gttaacgtca tataccctgc
      181 cagaatcgca accagccacg ggaaatagct taacggcagc gcctgcagct gcagataact
      241 ggccagcggt gaaaacggca atgcgatccc gacaatcatc acgatcacgg tcatgatcat
      301 taacggccac gatgcacagc tctgaataaa cggcacacgg cgggtacgga tcatatgcac
      361 aatcagcgtt tgcgacagta agcccaccac aaaccatccc gactggaaca gcgtttgcgt
      421 ttccggcgtg ttggcatgga atacccacca catcaggcaa aacgtcagaa tatcgaagat
      481 cgagctgatc ggtccgaaga agatcataaa gcgccccaga tccgccggat tccaacgttg
      541 cggcttttga atttgctcgt cgtcgacgtt atcaaacggg atcgccacct gtgacacatc
      601 gtacagcagg ttctgaataa gcaagtgtaa cggcaacatc ggcaggaagg gcaagaaagc
      661 actcgctacc agcacgctga acacattacc gaagttagag ctcgccgtca ttttgatgta
      721 tttcagcatg ttggcgaaag tgcgacgtcc ctcaataacc ccctcttcca gcaccatcag
      781 gcttttttcc agcaggatga tatcagccgc ttcacgggct atatctaccg cgccatccac
      841 agaaatgccg atatccgcag cgcgtaaggc gggcgcatca ttaataccat cgcccataaa
      901 gccaaccaca tgcccttcgc gcttcagtaa ggtcacaatg cgttctttat gcatcggagt
      961 cagacgagca aacagcgtgg tgcgctgtgc gagatttgcc agttcgtcgt cagatagcgt
     1021 ttcaatatca ctaccaatga ccacctctcc cgcgtccagc cccacttcat ggcacacttt
     1081 cgctgcaact aactcactgt cgccagtaag gatttttacg gtaattccac tcgcttttaa
     1141 tgccttcagt gccggagcag ttgtctcttt tggcggatca aggaaagcaa tatatccttc
     1201 gaggatcagg tcggattcat ccgcccgctg gtaatctcct tcacgcgctg gcaggtattt
     1261 cgtcgccacc gcaaccacgc gcagcccctg acgattcagc gtatcagtaa cccgcttaat
     1321 cttacgcagc atgatgtcat cgagcggcac aatctcgcca ttgtgacgca cctgcgaaca
     1381 cacattgagg atttcctgca atgcaccttt gcaaaccagc tgatggtgct cggtattttc
     1441 tgccactacc accgacatcc ggcgacgctc gaaatcaaac ggaatctcat cgattttttg
     1501 ccaacgactg gccagcgagc gcgctgactc ttcatccgta ccttcgagca ccgctgtatc
     1561 gagcaggttt ttaagtccgg tctgataatg actgttcaac cacgcgctat gcagcacgcg
     1621 ttcgctggtt ttaccggaga tatcggtatg attctccagc acaattttgt cctgtgtcag
     1681 ggtgccagtt ttatcagtgc acagaatatc cattgcgcca aagttctgaa tagcatccag
     1741 atgtttgacg atcactttct gtttcgacag ctttactgcg ccgcgcgcca gcgtcgaggt
     1801 aacaatcatc ggcaacattt ccggcgttaa gcctaccgcg accgaaagcg caaacagtgc
     1861 cgcttcccac cagtcgcctt tggtgtaacc attgattaac agcaccaccg gagccatcac
     1921 cagcataaag cgaatcagca gcatactgac gcggctgatc ccttgctgaa aggcattcgg
     1981 ctcgctttcc tgctcactaa cacgccccgc cagttgacca aaccaggtat tggcacctgt
     2041 agcaatcacc attgcttgtg ccgtgccgct caccacggtg gtgcccataa aacacagggt
     2101 gtcgcactcc agcggattgc tgtgctccgg ctggcgagtg gtagctgctt tttctacggg
     2161 cagagactca ccggttaacg acgcctgagc gacgaacaga tcccgcgcct gcaagatacg
     2221 taaatctgcc gggatcatat cccccgccgc cagttttata atatcgccgg gcaccagctg
     2281 gtcgatcggg atctccagcc agccattttc gcctttgtcg ttaattacgc gcagcaccgt
     2341 cgcagtattg ctgaccatcg ctttcagggc atctgccgct ttagtggaac gtgcttcctg
     2401 aataaagttc agcaacgtag aaatagcgac cattagcgcg ataacgcccg cggcaaataa
     2461 atcttccgtg gcgtaagaaa tagcgccgag aatggtgagt aaaatattaa atgggttgcg
     2521 atagcagacc cataaatgta cccaccacgg cgacggttgt tgtgcgggta atttattttc
     2581 accatgttgt tcgcgggcag attccacttc cgcctgattt aacccttccg gatgggtgtc
     2641 gaacgttttc cataattctt cttcgggcat caccgccatt ttcaggcaat gcgcacttaa
     2701 ggacggcggg accgccgtat tcactgtctg ctgcgcgcca ggcaatggat cgcgatgaac
     2761 cagacgggaa ggtaaatggc gaatgagccg ggtaaaaatt tctttaaaca taaggagtcc
     2821 ctccgcactg tctgaaacag tacagaaatg tcttcaggcg tggcgaagcc ttacctgaag
     2881 gtaataaagc tgagcaggga cgttaaaacg tcacggcctt acgcatccgg taacgccgtg
     2941 aaaagacggg cttaccggaa aagttttaat ctccgtcgag ggagaggcgt gggatcaggt
     3001 tccatataac ctccggtaag tgaaaaaagc gccgcaaata ataaggagaa tccaataata
     3061 ttcgtagcat ctatggcgat ataatacctg ctggcaacta aaccaacgat aaaccagact
     3121 ttacccattg ctgaatgcac gggtaaagtt aggctcaaat aattaaacaa cacgttacag
     3181 gacaacagga tgcaaaatcg gctgaccatc aaagacatcg cacgcttaag cggcgtgggg
     3241 aaatctacag tttcccgggt gctgaataac gaaagcggcg tgagccagcg cacccgcgag
     3301 cgtgttgaag cagtgatgaa tcagcatgga ttttcccctt cccgctctgc gcgcgctatg
     3361 cgtgggcaaa gcgataaagt ggtcgccatc attgttaccc gtctggattc gttgtcagaa
     3421 aatctcgccg ttcaaaccat gctgccagcg ttctatgaac aaggttacga cccaatcatg
     3481 atggaaagtc agttttcccc acaattagtt gccgaacatt tgggggtgct gaaacggcgt
     3541 aatatcgacg gcgtagtgct gttcggtttt actggcataa cagaagaaat gttagcccac
     3601 tggcagtcat cgctggttct gctggcgcgt gacgcaaaag gctttgcttc ggtctgttat
     3661 gacgacgaag gggcaatcaa gatcctgatg caacggctgt atgaccaggg gcatcgtaat
     3721 atcagttatc tcggcgtgcc gcatagtgac gtgacaaccg gtaagcgacg tcacgaagcc
     3781 tacctggcgt tctgcaaagc gcataaactg catcccgttg ccgctctgcc agggcttgct
     3841 atgaagcaag gctatgagaa cgtggcaaaa gtgattacgc ctgaaactac tgcgttactg
     3901 tgcgcaaccg acacgctggc acttggcgca agtaaatacc tgcaagagca acgcatcgac
     3961 accttgcaac tggcgagcgt cggtaataca ccgttaatga aattcctcca tccggagatc
     4021 gtcaccgtag atcccggtta cgccgaagct ggacgccagg cggcctgcca attgatcgca
     4081 caggtaaccg ggcgcagcga accgcaacaa atcatcatcc ccgccaccct gtcctgatcg
     4141 tttcctgaac gataaattgt gatcttcgct gcgtttcggg aacgttcccg tttttaaatt
     4201 tttccgcgca atatattctg cagccaacca aaaatgtcat ctgccatggg gctttatgat
     4261 gagcaaaata aaccaaacgg atatcgatcg gttgattgaa ctggtcggcg ggcgcggcaa
     4321 tattgcgaca gtgagccact gtattactcg cctgcgcttt gtcctcaacc aaccggccaa
     4381 tgccagaccg aaagaaattg agcaactccc catggtgaaa ggctgtttca ccaatgccgg
     4441 gcaatttcag gtggtgattg gcaccaatgt gggtgattac tatcaagcac taatagcgtc
     4501 aaccggacag gcgcaggttg ataaagagca ggtaaaaaaa gccgcccggc agaatatgaa
     4561 atggcatgag cagttgatct ctcatttcgc ggagatcttc ttcccgttgc tgcccgcgtt
     4621 gattagcggc ggtttgatcc tcggttttcg caatgtgatc ggcgatttgc ccatgagcaa
     4681 cggtcaaacg ctggcgcaaa tgtacccttc cctgcaaacg atctacgatt ttctgtggtt
     4741 gatcggtgaa gcgatcttct tctacctgcc ggtcgggatt tgctggtcag cggtgaaaaa
     4801 aatgggcggc acgccgatcc tcggtatcgt gcttggcgtg acactggttt ctccacagct
     4861 gatgaacgct tatctgctcg ggcagcagct gccggaagtg tgggactttg gcatgttcag
     4921 catcgccaaa gtaggctatc aggcgcaggt gatcccggca ctgttagccg gactggcgct
     4981 gggcgttatt gaaactcgcc ttaaacgcat cgtgccggat tacctctatc tggtggtggt
     5041 acccgtctgt tcgctgatcc tcgcggtgtt cctcgcccat gcgctgattg gtccgtttgg
     5101 tcgcatgatt ggcgatggcg ttgcctttgc ggtacgtcac ctgatgaccg gcagctttgc
     5161 tccgattggt gcggcattgt ttggcttcct gtacgcgccg ctggtgatca ccggcgtaca
     5221 ccagaccacc cttgctattg atttgcagat gattcaaagc atgggtggca cgccagtgtg
     5281 gccgctgatt gcgctgtcga atatcgctca gggctccgcc gtgataggca ttatcatttc
     5341 cagccgcaag cacaatgaac gcgagatctc cgtgcctgcc gctatctccg cctggcttgg
     5401 ggtcactgag cctgcaatgt acggcatcaa cctgaaatat cgcttcccga tgctgtgcgc
     5461 gatgattggt tctggtctgg caggattgct atgcggcctg aacggcgtta tggcgaatgg
     5521 catcggcgta ggcggcctgc cgggaattct ctcgattcaa ccgagctact ggcaggtgtt
     5581 tgcgctggca atggctatcg ccatcatcat cccgattgta ctcacctcgt ttatctatca
     5641 gcggaaatac cgcctgggca cgctggacat tgtttaattt tcttcggggc gcaattgcgc
     5701 cctctcgcat ttgcaggaat aacgtaatga ctaatcttcc ccactggtgg caaaacggcg
     5761 ttatctacca gatttatcca aagagttttc aggacaccac gggtagcggc accggcgatt
     5821 tacgtggcgt tatccaacgc ctgggctatc tgcataaact gggcgttgat gccatctggc
     5881 taaccccctt ttatgtctct ccccaggtcg ataacggtta cgacgtagcg aactatacgg
     5941 cgattgatcc cacttacggc acgctggacg attttgacga actggtgacg caggcaaaat
     6001 cgcgcgggat tcgtatcatt ctcgatatgg tgtttaacca tacctctacc caacatgcct
     6061 ggtttcgcga ggcgctgaac aaagaaagcc cttaccgcca gttttatatc tggcgcgatg
     6121 gcgaaccaga aacgccaccg aacaactggc gttcaaaatt tggcggtagt gcgtggcgct
     6181 ggcatgcgga aagcgaacag tactatttgc atctctttgc accagagcag gcggatctca
     6241 actgggagaa tccagcggta cgcgcagagc tgaaaaaagt ctgtgagttc tgggccgatc
     6301 gtggggtcga cgggttgcgc ctggatgtgg tgaatctgat ctccaaagac ccacgtttcc
     6361 ctgatgacct ggatggtgac gggcgtcgct tctacaccga cgggccacga gcacacgagt
     6421 ttttgcacga gatgaaccgc gatgtgttta cgccacgcgg gttaatgacc gtaggtgaaa
     6481 tgtcctccac cagccttgag cattgccagc gatacgcggc actgacaggc agtgaattgt
     6541 cgatgacctt taattttcat cacctgaagg tcgattatcc cggtggtgaa aaatggacgc
     6601 tggctaaacc tgactttgtg gcgttgaaaa cattgttccg ccactggcaa caaggaatgc
     6661 acaacgtggc atggaatgcc ttgttctggt gtaaccacga tcagccgcgc attatttctc
     6721 gctttggtga tgaaggtgaa taccgcgtgc ctgcggcaaa aatgctggcg atggtgctgc
     6781 atggcatgca gggaacgccg tatatctacc agggcgaaga gattggcatg accaacccgc
     6841 atttcacgcg cattactgac tatcgcgacg tggagagcct caatatgttt gccgagctgc
     6901 gcaacgatgg tcgtgatgcc gacgagttat tggcaatcct cgccagtaaa tcccgtgaca
     6961 acagccgcac gcccatgcaa tggagcaacg gcgataatgc cgggtttacg gctggcgaac
     7021 cgtggattgg tctgggcgat aactatcaac aaatcaacgt agaagccgcg ctggccgatg
     7081 agtcctcggt gttttacacc taccaaaagt taatcgcact gcgtaagcaa gaagccatcc
     7141 tgacatgggg caattaccag gatctgctgc caaacagccc tgtattgtgg tgctatcgcc
     7201 gtgaatggaa ggggcaaacc ttgctggtca ttgccaacct tagccgtggg atccaaccct
     7261 ggcagccagg gcaaatgcgc ggcaactggc agcttgtgat gcataactac gaagaagcct
     7321 caccacaacc ctgtgccatg aatttacggc cttttgaggc tgtctggtgg ttacagaagt
     7381 aaatcttccc taagccccgg taatgccggt cattccgggg ttttgtctgt cagcatgttg
     7441 tttttgttga tttttcaacc agcaaattca ttaaaaaatt tacataacgc tgtagcgccc
     7501 gtcatccgta cgctctgctt tttactttga gctacatcaa aaaaagttca aacatccttg
     7561 atgcaaagca ctatatatag actttaaaat gcgccccaac ccaatatgtt gtattaatcg
     7621 actataattg ctactacagc tccccacgaa aaaggtgcgg cgttgtggat aagcggatgg
     7681 cgattgcgga aagcaccgga aaacgaaacg aaaaaaccgg aaaacgcctt tcccaatttc
     7741 tgtggataac ctgttcttaa aaatatggag cgatcatgac accgcatgtg atgaaacgag
     7801 acggctgcaa agtgccgttt aaatcagagc gcatcaaaga agcgattctg cgtgcagcta
     7861 aagcagcgga agtcgatgat gccgattatt gcgccactgt tgctgcggtt gtcagcgagc
     7921 agatgcaggg ccgcaaccag gtggatatca acgagatcca gaccgcagtt gaaaaccagc
     7981 tgatgtcggg tccatacaaa caactggctc gtgcttacat cgagtaccgt cacgatcgcg
     8041 acattgaacg tgaaaaacgc ggtcgcctga accaggagat ccgtggtctg gtcgagcaga
     8101 ccaacgcctc gttactcaac gaaaacgcca acaaagacag caaggtgatt ccaacccagc
     8161 gcgacctgct ggccgggatc gtggctaaac actatgcacg ccagcacctg ctgccgcgtg
     8221 acgtggtgca ggcacatgag cgtggcgata ttcactatca cgatctcgat tactcgccgt
     8281 tcttcccgat gttcaactgc atgttgattg acctgaaagg catgctgacc cagggcttta
     8341 aaatggggaa cgccgagatt gaaccgccga agtcgatctc tacggcaacc gctgtaactg
     8401 cgcagattat tgctcaggtc gccagccata tttatggcgg caccaccatt aaccgtatcg
     8461 atgaagtgct ggcaccgttt gtcaccgcca gctacaacaa acatcgcaag accgcagaag
     8521 agtggaacat cccggacgcc gaaggctacg ctaactctcg taccattaaa gagtgctacg
     8581 atgccttcca gtcgctggag tacgaagtaa acaccctgca caccgccaac ggtcagacgc
     8641 cgtttgtaac ctttggtttt ggtctgggca ccagctggga atcgcgcctg attcaggaat
     8701 ctatcctgcg taaccgtatc gcaggcctcg gtaaaaaccg taaaactgcg gtgttcccga
     8761 aactggtgtt tgcgattcgc gatggtctga accataaaaa aggcgatccg aactacgaca
     8821 tcaaacagct ggcgctggag tgcgcaagca agcgcatgta tccggatatc ctgaactacg
     8881 atcaggtagt gaaagtcacc ggttcgttta aaaccccgat gggctgccgc agcttcctcg
     8941 gcgtgtggga aaatgaaaac ggcgagcaga tccacgatgg tcgtaacaac ctcggcgtga
     9001 tcagcctgaa cctgccgcgt attgctctgg aagcaaaagg cgatgaagcc accttctgga
     9061 agctgctgga tgaacgtctg gtgctggcac gtaaggcgct gatgacccgt atcgctcgtc
     9121 tcgaaggcgt gaaagcgcgc gtggccccga tcctctatat ggaaggtgct tgtggcgtgc
     9181 gtctcaatgc tgacgatgat gtttctgaaa tcttcaaaaa cggtcgtgcg tctatttcgc
     9241 tgggttacat cggcatccac gaaaccatta acgcgctgtt cggcggcgag catgtttacg
     9301 acaacgagca gcttcgcgcg aaaggtatcg ccattgttga acgtctgcgt caggcagtgg
     9361 ataagtggaa agaagaaacg ggttatggtt tcagtctcta cagcacgccg agtgaaaacc
     9421 tgtgcgaccg cttctgccgt ctcgatactg ctgagtttgg cgtggtgccg ggcgtgaccg
     9481 ataaaggtta ctacaccaac agtttccacc tcgatgtgga gaagaaggtg aacccgtacg
     9541 acaagatcga ctttgaagcg ccttacccgc cgctggcgaa cggtggtttc atttgctacg
     9601 gcgagtatcc aaacattcag cacaacctga aggcgctgga agatgtctgg gattacagct
     9661 atcagcatgt accgtattac ggcaccaata caccgattga tgagtgctac gagtgtggct
     9721 ttaccggtga gttcgagtgc accagcaaag gcttcacttg cccgaaatgt ggtaaccatg
     9781 acgcctcccg tgtgtcggtg actcgccgcg tgtgcggata tttaggtagc ccggatgcac
     9841 gtccatttaa cgccggtaag caggaagaag ttaagcgccg cgttaaacat ttgggaaatg
     9901 ggcagatagg ttaatcttca ctctgcgcta agcaaatgcc ggatgcggcg taaacgcctt
     9961 atccggccta catttggcaa cggctccagg cctgataaga cgcgacagcg tcgcatcagg
    10021 cattgattac cggatgcagc ctacacgcgg actgataagc caggcgaaat aatgaattat
    10081 catcagtact atcctgtcga catcgtcaac ggccccggca ctcgctgcac cctgtttgtc
    10141 tccgggtgtg ttcatgaatg ccccggttgc tataacaaaa gcacatggcg ggtaaattct
    10201 ggtcagccat ttaccaaagc aatggaagac aagatcatta aagatctgaa tgacactcgt
    10261 atcaaacgcc aggggatttc cctctccggc ggcgatccgc tgcatccgca aaacgtgccg
    10321 gatattctga aactggtaca acgcatccgc gccgagtgtc cgggtaaaga catctgggtg
    10381 tggacaggct ataaactcga cgaactcaac gctgcgcaaa tgcaggttgt tgatttgatt
    10441 aacgtgctgg tcgacggcaa atttgtgcag gatttaaaag acccttccct gatctggcgc
    10501 ggcagcagca atcaggtggt gcatcatttg cgatgataag ttccctgtta cctgagtgca
    10561 ggcaacaggg aaatgaggaa ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc
    10621 agttgctctg cagcagcctg cgcttctttt actttacctt catttgccag cttcagcgcg
    10681 tcgtcaatct gaccgaccag aatgtcgaaa ccgtggcgga aatctttcat ttccgggctg
    10741 tccggtgatt tatcttcgag cttcggcggc gttgcttttt gcgcatccag cgctgcggcg
    10801 cgcatcttcg ttaacgcgtc tttgacttgc gtcgcgttat cggctttttc gaccactttt
    10861 aaattgtcgt tgagggtttc catattgtct tcaagatcag cggcaaacga cgccgaactg
    10921 aataccaacg aggagactgc aagaatagct aacaggcttt tacgcattgc tcacttcctt
    10981 ttttatttac acttcatcct tcacactgcc tcatcgacgg ctacgctcat tcgcccgggt
    11041 cacgtactgc gagtacgctc cccggccctc attcactttc cgtctcgatg cagtgcgaat
    11101 gattttgtgt aattttttag tttatagcgc ggcaggtcgc gccagttttt tactgcccgg
    11161 cgattttcat ctccggcaac agcacagaac cacactgtat attactgcgt gtttcaatat
    11221 cgttaccgac ggtgacaata ttgcgccaca tatcttttaa attacctgcg atggtgattt
    11281 cactaacggg atactgaatt tcgccattct ctacccagaa gcccgccgca ccacgggaat
    11341 aatcaccggt aattgcactc acgccctggc ccatcaattc cgtcaccacc agcccggtgc
    11401 ccatctcttt gagcatctgc tcgaagctta gaccttgtcc ggcaatccgc cagttgtgaa
    11461 taccgcccgc atgtccggtg cttttcagcc ccagttttcg cgccgaatag ctggtcagca
    11521 gccactgagt cagggtgcca tctttaataa tatcgcgacg ctcggtgcgc acaccttcgc
    11581 tgtcgaatgg cgtcgatgcc agccctttca gcagatgcgg atgctcttca atggtcagcc
    11641 agtccggcag aatttgttta cccagcgaat ccagcaggaa ggtggattta cggtaaaccg
    11701 atccacccgc tatcgccccc accagatggc caaaaagccc ggttgccact tcattggcaa
    11761 aaatgaccgg cgctttcatg gtggagagtt tacgcggtga caggcgcgat aaagtacggc
    11821 gagcacagtc ggccccaacc cactctggcg tttgcagatc gctcatcgca cgaccaatgg
    11881 tgtaggcgta atcacgctcc atatcgccat tttcttcggc aattacacag ctggagagcg
    11941 aatgacgcgt tgagcagtaa ccctgcaaca tgccgtggct gttgccaaaa actttgacac
    12001 cgtagtggct gttaaagctg ccaccttcgg tattggtgat gcgtttgtcc gcctgcaatg
    12061 ccgcctgttc tgcgcgggcc gccagttcaa tggcttcatc cggggaaact tccgcagggt
    12121 ggaacaagtc gagatctggt gcgtcaaagg ccagcagctc tttgtctgcc acgccggcac
    12181 agggatctgg cgaggtataa cgggcaatat ccagcgccgc ctgtacagta cgggcaatgg
    12241 cctgcgggct taaatcggtg gatgatgcgc tacctttgcg gttctggtga taaacagtga
    12301 tccccagcgc gccatcgcta ttgaattcaa cattctccac ttcaccataa cgcgtgctta
    12361 cgctaatgcc ggtggtcttg ctgacggcaa cttccgcacc gtccgatttg cctgaggcca
    12421 actccagcgc agtggaaact gcttcttcca gaatcttgcg ctgcgcttca acttgagaga
    12481 ttactttcat tgcaagtgcc ataatgtaga gagtttctct gaagtctaac agagaaccgt
    12541 ttttcagtgc gcatcttaac tggtaacatt agcctctttt tttaaggagc ctgagatgac
    12601 taagcagccc gaagactggc tcgacgacgt tcccggtgat gacatcgaag acgaagacga
    12661 tgaaattatc tgggtcagta aaagtgaaat taaacgtgat gccgaggagc taaaacgcct
    12721 tggcgcggaa attgttgatc tggggaaaaa cgcgctggat aagatcccgt tagatgcgga
    12781 tctgcgcgcg gctattgagc tcgcccagcg tattaagatg gaaggtcgcc gccgccagtt
    12841 gcagctcatt ggtaagatgc tgcgccagcg cgatgttgaa ccgatccgtc aggcgctgga
    12901 taagctgaaa aaccgtcaca accagcaggt ggtgctgttc cataaactgg aaaacctgcg
    12961 cgatcgtctg atcgaccagg gtgatgacgc catcgctgaa gtattaaatc tgtggcccga
    13021 tgccgatcgt cagcaactgc gtactctgat ccgtaacgcg aagaaagaga aagaagggaa
    13081 taagccgccg aaatccgcac gccagatttt ccagtatctg cgcgaactgg cggagaatga
    13141 agggtaattt tcgttgttga tgtgagtatg gtttgccgga tgcggcgtaa acgccttatc
    13201 cggcaatgct atctcaggct aaggccgaat tactgcgcag cttccgcctt cttcgccaga
    13261 ccatccagca gtttctgatg gatcccacca aaaccgccgt tgctcatcac cagaatatgg
    13321 tcgccaggct gagcggtttt caccaccata tctgccagcg tatccacatc gccactccag
    13381 tgtgcaggct gaacgcaggc ttctgccact tctgccacct gccacggaat atgcgtcggt
    13441 tgcagcagga agacttcatc ggcacggcct aatgaaggtg ccagatcgtc tttgcagatc
    13501 cccattttca tggtattcga gcgcggttcc agcacagcaa taatgcgcgc cgtaccgcca
    13561 actttgccac gcagcgccgc cagcgttgcc agaatcgccg ttgggtgatg ggcgaaatca
    13621 tcatataccg tgacgccatt cgcttcacca cgcagttcca gacggcgacg agcgttaata
    13681 aacgaaccca gcgcattagc ggcatctgcc ggcgctacac caacatggcg agccgccgca
    13741 atcgccatca ggccattgtg catattatgt tcgcctacca gcgaccattt cacttcgccc
    13801 actttttcgc catccagcaa gacttcccat tcggaagcat cgatggtcag ctttttcgcc
    13861 tgccagtgac cctgctcacc caccagctcc tgctcgctcc agcagcccat cgccatggtc
    13921 tgtttcaggt tgatgtcatt ttccggccag ataatacggc cctgccccgg tacgatacgc
    13981 accagatggt ggaactgttt ctggatcgct ttcaggtcgt caaagatatc ggcgtgatcg
    14041 aactcaaggt tgttgaggat cagcgtacgc gggcagtaat gaacaaattt agagcgtttg
    14101 tcgaagaagg cgcagtcata ctcatccgct tcgataacaa agaagtcgct ttcgcccaga
    14161 cgcgccgaaa cctcaaagtt ccccggcaca ccgccgatta caaagcccgg tttgtaacca
    14221 cactgttcca gaatccaggt cgccattccc gcggtggtgg ttttgccatg tgtaccggca
    14281 acggccagca cccagcggtc gcgcagcaca aaatcgtgca gccactgtgg acctgacata
    14341 taagggatgt ttttttccag taccgcttcc acacacggat ttccacgggt catggcgttg
    14401 ccaataatca ccagatccgg ctgcggatcg agctggctgg catcgtaacc ctgaatcagt
    14461 tcaatgcctt gcttctcaag taaggtgctc atcggcggat acacattggc gtccgaaccc
    14521 gttacttcat ggcctaactg gcgcgccagc atcgccagac cgcccataaa cgtgccacaa
    14581 attcctaaaa tatgaatgcg catacgtcac tatccttttt taatctgccg ctcattttac
    14641 gcatatgtcg ggcaagtgag aaacgcattt caggataatc cgcaatttgc tggcgcgatt
    14701 cacctgagcg caacattccg attatttgtt aagattgtta cggtcgcttt actccataaa
    14761 cattgcaggg aaagttttat gaaaacgtta ggtgaattta ttgtcgaaaa gcagcacgag
    14821 ttttctcatg ctaccggtga gctcactgct ttgctgtcgg caataaaact gggcgccaag
    14881 attatccatc gcgatatcaa caaagcagga ctggttgata tcctgggtgc cagcggtgct
    14941 gagaacgtgc agggcgaggt tcagcaaaaa ctcgacttgt tcgctaatga aaaactgaaa
    15001 gccgcactga aagcacgcga tatcgttgcg ggcattgcct ctgaagaaga agatgagatt
    15061 gtcgtctttg aaggctgtga acatgcaaaa tacgtggtgc tgatggaccc actggatggc
    15121 tcgtccaaca tcgatgttaa cgtctctgtc ggtaccattt tctccatcta ccgccgcgtt
    15181 acgcccgtcg gcacgccggt aacggaagaa gatttcctcc agcctggtaa caaacaggtt
    15241 gcggcaggtt acgtggtata cggctcctct accatgctgg tttacaccac cggatgcggt
    15301 gttcacgcct ttacttacga tccttcgctc ggcgttttct gcctgtgcca ggaacggatg
    15361 cgcttcccgg agaaaggcaa aacctactcc atcaacgaag gaaactacat taagtttccg
    15421 aacggggtga agaagtacat taaattctgc caagaagaag ataaatccac caaccgcccg
    15481 tatacctcac gttatatcgg ttcactggtc gcggatttcc accgtaacct gctgaaaggc
    15541 ggtatttatc tctacccaag caccgccagc cacccggacg gcaaactgcg tttgctttat
    15601 gagtgcaacc cgatggcatt cctggcggaa caagcgggcg gtaaagcgag cgatggcaaa
    15661 gagcgtattc tggatatcat cccggaaact ctgcaccagc gccgttcgtt ctttgtcggc
    15721 aacgaccata tggttgaaga tgtcgaacgc tttatccgtg agttcccgga cgcgtaatct
    15781 cctgcgcctc tccacacgga gaggcgtttt tatcgctgcg caatgttgac tcttgtcact
    15841 ggcgaactct gacgattctc ccacagcacc gttaatccac gctgtaatgc tataaaaata
    15901 aacaacaaaa taccgatggc gattttcgtc caccatgagc tcagcgtgcc atcaaagttt
    15961 atgtaagtct gaatcagtcc ctgaatcgcc acgccaaaaa gcgtccctaa taccgttcca
    16021 acgccaccgc tcaaaagcgt accgccaatc accaccgacg cgatggcgtc cagttccaca
    16081 cctacgcccg ccagcgcata tccggcctgg gtataaatcg agaagacaat acccgccagc
    16141 gttgccagtc cggtggagag catataaatg cgaatagtgg tgctgcgagt ggaaatcccc
    16201 atcaggttcg ccgacgttgc gttgccgcca atggcgtata cctgattacc aaagcgggtg
    16261 cgatgcgcga ggaaaatacc gataaccacc accgccaaca tcagcagtcc catcgcactt
    16321 aagcgaccgc cgccagggat tttccacgca aggcttgaga gcgtgtcata aatgggatgg
    16381 tttatcggaa tcgactcttc cgaaacgaga tagctgacac cgcgcaaaaa gaacatcccc
    16441 gccagggtaa tgataaatgc cgggatcttc agggcgtcga tcaaaagccc cataaatgcg
    16501 ccgaaggcac agcccatcac cagcactagc ggaaacgcca gtagcggcga gaggccgaaa
    16561 tcgccaatca cttttgccag aaacacgcca gtaaaggcga tcaccgaacc gacggagaga
    16621 tcgatcccac cggagaggat cacaaaggtc atgccaacgg caatgatccc aagaaaggcg
    16681 ttatcggtca ggatattgca gatcactctt gtggaagcaa aaccgggaaa ctgggtcagg
    16741 cagtaaagat aacccaacac aaagacgccg atggtgatca tcagtggcaa tttacgtttt
    16801 atcacggcta cgtactcctt taatcagact gataaagcgt tgcgactgga caatcagcac
    16861 gcaaagaacc accaccgctt ttacaacctg gttcatctct ggcggaaagc ccgaaagcaa
    16921 aattccggtg ttcatcccct gaataatcag cgcccccacc accgaaagca gtaggttaaa
    16981 acgtccgccc atcagcgatc cgccgccaat aaccaccgcg agaatggcgt ccagctccag
    17041 ccataacccg gcgttattgg catcggcacc gcgaatatcc gccgccacga taatgcccgc
    17101 aatcgccgca cacagcccgc tcaacacgta ggtaagcatg acgatgatcc gcgtgtttac
    17161 cccggcattt tttgccgccc gaatgttgat accaacggct tcgataaaca tccccagcgc
    17221 cgttttgcgg gtcaacagcc agaacaggat aagcgtcagc accgcgataa tgaccggcgt
    17281 tggcaggaac aacagcgatc cactgccaaa ccatgagaga tccggcgagt taaacgtgac
    17341 gatctgtccg gaggtgatca gttgcgccac gccgcgcccg gcgaccatca ggatcagggt
    17401 ggcaacaaac ggctgaattt tgaggatcgc taccagtatg ccgttccaca atcccgccag
    17461 gatgccagtg cccagggcgc ttaacaaaac aatcggcagg ctgaatcccg cgaccgtcat
    17521 cgcagccgtt gtggctccgg cgatagccat caccgccccc acggagagat caatcccacc
    17581 tgttgcgatc accagcgtca ttccgatagc caacagcgca acgggggccg cacggttaag
    17641 aatgtctatg gggctaccga ataaacgccc atcctggagc accacctgcc agaaatgcgg
    17701 ggccaccagg ctatcgacca gcagcaccag caacagtgcc gccagctgcg gcattcccgt
    17761 tggccagcga aagcgccttt tcggcggcgt agtgtccggg agagattgag gcatcacact
    17821 gttctcctta cgccgcaatg gcgttcatga tcgccggaac ggaaagctct gccagcggga
    17881 tctccgccac ctgtttgcga tcgcgcatga tgatcacccg gtcggcatag cccaccagtt
    17941 cttccagttc ggaggagatc accagcagcg ccagaccatc ggcgcatagc gtttcaatca
    18001 ggcggatgat ctcggcgtgg gcaccaacat caatgccgcg agttggctca tcaaggatca
    18061 gaaattgcgg tcgggtcagt agccaacgtg aaagcaacac tttttgctga ttgccgccag
    18121 agagaaattc aatcggttgt tcagttgaag gtgtgcgaat gccaagctgg cggataaagc
    18181 gttcggcaat ctcttgctgt gctttgcggg aaatgggacg tagccagccg cgctgggcct
    18241 ggagagcgag gatgatattt tcccgcaccg aggcggcagc gatgatgcca tcggttttcc
    18301 tgtcttccgg gcagaagcca atgcccagta ccgaagcctg atgtggcgat cgcaggtttt
    18361 gcggtttgcc tttgatcaac gccgtgccgc tgtcagcagg tttgataccg aagatcactt
    18421 cggcggtttc ggtacgtcct gatcccagca atccagccag accgacgatc tcgccggggc
    18481 gtacttcgag atcaaacggt gcgatcgttc cttttttgcc gtaatttttg aacgcggcaa
    18541 cgggtttgtc gctcaacaat gttcgcccgg cacgctgtag cgcgtgggta tccagctcgc
    18601 gccccagcat catttttacc agttcgatct gcggtagctc gcacgtttcc cgacagccta
    18661 cgaaactgcc gttgcgtaag acggtgatcc gatcgctgac ctgatagacc tgatcgagaa
    18721 agtgagtgac aaaaatcagg ctgacgccgc gatcgcgcaa ctgacgcatc aggtcaaaca
    18781 gtaactccac ttcctgggtg tcgagactgg cggtgggttc atcgaggatc agcactttgg
    18841 cagagagatc gatagcccgg caaatagcga cgatttgctg catcgcgact gaaaagcggt
    18901 tgagcggttc gcgcacgtcg agggagaaac cgtaagatgc catcagttcg gtggcgcgct
    18961 tttccatctc tttgcggcgt agaaggccga agcgtttggg ttcgcggcct ataaatagat
    19021 tatcagcgac cgacatattg ggtagcaggt ttacttcctg atagacggtg ccgataccga
    19081 gttgttgtgc atgggcggta tttttcggtg agatagcctg gccttccagc cagatggtgc
    19141 cgcgatcggc gtggtataca ccagttaatg ctttgattag cgttgatttt cctgccccgt
    19201 tttcaccgag cagcgccatg atttcgccgc ggcgcaggct gaaatcaacg ttgtctaacg
    19261 ctttgacgcc ggggaaaaat ttacttaatc cttcggtgcg gaggatctcc tggtgttggt
    19321 tagtcgtcat ggtttccccc ggtgtaaagc gtgctcccag taggcatgat aagacgcggt
    19381 aagcgtcgca tcaggcttca gcgcaccacc gccggatgcg gcgcgagcgc cttatccggc
    19441 ctacgaatcg gcatcaggca tagcaaccct caatacccca tatttttctt cttctctaac
    19501 tcttcttttg cggtatcagg cagataaagg gtggatttgg tcagcgtcag cttttcaggc
    19561 atggtgccgt cttttttgta tttctccagc gcgtcgaagg cgggacctgc catattcggc
    19621 gtcagttcaa cactggcgtt cgcttcgcca tcaatcatcg ctttgtagat atccggtacg
    19681 ccgtcgatag aacctgtcag aatatctttg cctggtttca ggcccgcttc tttaattgcc
    19741 tgaattgcac caatcaccat gtcgtcgtta tgggcgtaaa ccatgcagat gtttttgccg
    19801 ttgttttccg ctttgataaa gctctccatg acttctttgc ctttactgcg ggtgaagtca
    19861 cctgactgcg agcggataat tttgatattt ggcgcattct taatggcttc ggcaaagcct
    19921 ttcttacggt caatggcgac gctggcccca acagtgccct gcagctccac cacgttgcat
    19981 ggtttgccat tcacttcttt taccagccag tcaccaatca acttgccttc gagaatgttg
    20041 tcggcagtga cggtggtcat atagagagat ttgtctttca catcaataga acgatcgagc
    20101 aagaagaccg ggatttcggc atctttcgcc tcttttaata ccggttccca accagtcgca
    20161 accaccggag cgataaagat cgcatccacc ccttgcgcga cgaaggaacg taccgcttta
    20221 atctggtttt cctgcttttg ctgaccatcg gcaattttca gcgtaattcc gcgtttttcg
    20281 gcctcacttt tcgccacatt ggtttctgcg gcgcgccagc cagattccga tccgacctgc
    20341 gaaaatccta cggttaatgg agcggccaac gccatagacg acatggctgc cgagactgca
    20401 gagactacaa gtaagcgttt ccacataggg ttgtcctcgt cggggtaatt attattggtg
    20461 aaaagatgtt cgcgaaaaaa ctatagacaa ttcgttatgt aacggattgc gttacatcac
    20521 acttcaaaga agtaaataaa acgttaatca caagtttgta atcgctttca tctcactatg
    20581 aaaaatgcgg ctacggttat ggattttcct gctctgtata ccgtcttaaa attggcgaaa
    20641 aaggaaaatg aagacgaaaa caagcgaaga cattcggcgc gagttggcta taatactcgg
    20701 cacttgtttg ccacatattt ttaaaggaaa cagacatgag cttactcaac gtccctgcgg
    20761 gtaaagatct gccggaagac atctacgttg ttattgagat cccggctaac gcagatccga
    20821 tcaaatacga aatcgacaaa gagagcggcg cactgttcgt tgaccgcttc atgtccaccg
    20881 cgatgttcta tccgtgcaac tacggttaca tcaaccacac cctgtctctg gacggtgacc
    20941 cggttgacgt actggtcccg actccgtacc cgctgcagcc gggttctgtg atccgttgcc
    21001 gtccggttgg cgttctgaaa atgaccgacg aagccggtga agatgcgaaa ctgattgctg
    21061 ttccgcacac aaagctgagc aaagaatacg atcacattaa agacgttaac gatctgccag
    21121 agctgctgaa agcgcagatc gctcacttct tcgagcacta caaagacctc gaaaaaggca
    21181 agtgggtgaa agttgaaggt tgggaaaacg cagaagccgc taaagctgaa atcgttgctt
    21241 ccttcgagcg cgcaaagaat aaataagttc ttctagcgca ataaccctga acgccgggct
    21301 tcggttagta agggtttttt tatgcccgcg ataaataaac tctctattcc accatcatta
    21361 ttctcagcgg ttgcaaggct tgaacggtaa gaacaagcaa acccgaccac cattttgctg
    21421 ttcatagcca cttgctggaa gttagccgac ctcactcata ctcaccgttc aaagcgtgcc
    21481 cccgttttac ccctgcacat ttgtgaacgc cagcagagtt ttttatgcga ttgcagatat
    21541 ggaaagcggc tcggagagaa aagcaaaaga tggaaaagtt acaaaaaaaa atcgcgagag
    21601 acatcgcata aaacaacgcc acccgaaggt ggcgtgcttt tattattact tatccctgtc
    21661 taaccagtcg ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat aaacgtacat
    21721 ccatgcactc ccgtacggcg tctgaatcaa ctggcgcgcg tattcaccgc ccctggtgcg
    21781 caaggcatca agttcggcca gcgtggcgtt gtcaatacga taaacttcac cgtgtactgt
    21841 tccgttcccc ggaactgcgc ctggatagtg gcccaggcta tacaactggt agttatcgat
    21901 actgaaatcg cccagtaact gggcattggt catccagtga ctgttgcctt gtttgtggcg
    21961 taaactgccg tagacaaata ttcgcattgc taaaactcga actgatagag caaatccagt
    22021 gcctggtcta caccagacac ggcttccaga tatagcttag gcatcaggcg ataacgtaac
    22081 gtgagtgttg ctatagagtc aaatataccc acgccgtatt tcacttgcag acctggcaat
    22141 acatagccgc tgaccactac ctgggaggag tcgcctactc cctgggtgtc gagcgctaaa
    22201 ttgcttacgc caaacgtctc gccgatttta cccacaatct ggccactttg cgcaaccccc
    22261 agaccaatca gcatcgaggt cattgccgca ctgtcgctct gatcgctctc cagcccttgt
    22321 ccacgtagca aataagacaa tgcagcttgt tgcgacatcg ccgggtcaga gaagatctcc
    22381 gctttcggtt cgtccgccag accagtgacg cgaactccgg cgattacgtc gtcttctgta
    22441 gcatccgggt tacgaatagc ttcaatatta agatacggtt gatccggcgg accagagaac
    22501 agtaactcac ctttacgcac aatcagatcc tgaccatagg catggaagcg cccttcaggg
    22561 atgttgatct gcccgttcag gcccagccct tgtttgtcct gaacgacgtt aagatcgccc
    22621 gtcagccgcg ctttcaggcc aaaggcgtca atgcgcacat tgttgccaac gtggacaatc
    22681 aggttactgt taatcggaat cgacgccgtt ttcggctctt ccggttgcag gttatcgtta
    22741 agcatcacca catcgctgga gacgcctact gcgctttccg gcagatcgtg caccacgatg
    22801 cgcgcccacg ggacatccac gcgaccatcg agggtaaaca ggtttggtgt agcctcgaag
    22861 acaacatctg gcgatacatc cattcgtacc atcggcggca cggtgatccg cactttactg
    22921 cctttcgccg ttacccgcgc ccgccagttt tcaatttggc tccagtcggc atcaccgttc
    22981 aggtagattt caccctgctg ggtccgtact gtaccggcaa gcgtcgagcg cataccatta
    23041 aagttgaccg caagctggct cggttgcata tcaaacggca taaagttgcc gtcgatatcc
    23101 acacccgtaa cctgaagctg accaaacaac tgcgggcttt gcacatcacc acccagacgc
    23161 aagttggcac tcaccatccc cgctgctttt tccccacggg taaagatggg gtttatcatc
    23221 gcaaggttga agttacggat attgacgttg ccaccaagat tacggcggcc ttgcggatcg
    23281 gtcacctgca cctgtccatc aaactggccg ttattggtca ggcggatggt ccagcccaat
    23341 tcggcacggt tgttacgcaa ttccgccgtc agattcagtg tctgaaacgc cacgggcagc
    23401 gccgcatcgt tgacggtttg cgttacctgc acgttacgcc ccgaaagggt gatactgccc
    23461 tgcggcagcc cctctttggt ggtgtcccag gcaacgtccg ctttacccgt gaagataccg
    23521 ctggcctgag tggtttctgg cataaatggt ttcagcatgg ccaggtcgaa gcggttgaga
    23581 ttcaccaccg cacgcccttc ggcacccgca tcgatagttt gcggcacgca cagttccgca
    23641 ttcgggttaa gccagcagtg tggcccgatg ctgatttttt gctccttatt gcggtaatcc
    23701 agcgcaatat cgcgggtcag cgaccacggg ccgaccggcg tctggaagcg ggtattgcta
    23761 agagttccct tccagcgttc ttctttgcga tcaaaacttc ctgccagatt aagctgcccg
    23821 gagacaggct cgccctgaat ccgcaactgt aactcgtgct gtttttcgct gcctttggca
    23881 ttcagggtga cgaggttgat atttacatcc ggctgcgaaa tttgctcaac gcgtacgtcg
    23941 agtttcccgg cgatctgatc ggtggattta atgtcgcctt ccacgcgaac ctgcgccacg
    24001 gaaagttcct gccagcgcag gccgcgcgcg gtgatatctg ccagtagttg tggcgcttcc
    24061 accgtgccgc gtactttcac cagccctttc gctgtaccgc caagccccgg cagcgcgtta
    24121 tccagccccg gcgcgttgat ggtggcatca agattgagat cttttacccc cagctcgcct
    24181 ttcacttcgg cactgtttgg ccccagttcc agatgaagcc ctgggatcat ccactgcata
    24241 taactgttgc ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac gttaccggtc
    24301 agcttcagtt ctggcacctc catctgccag gtgccgccgt acaggctacc gcgggtttta
    24361 atcaagccat tgagtttcga cggccactct gggatctctt tggcggtgtt aatgccgtta
    24421 agcgttagct caccgcgcca actaatggcc tgctgccagt cgagcaacgc cttgagttca
    24481 gttttccctt ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg ttcattacct
    24541 ttggcatcaa gggtaatggt ggctggcggg atctccagtc ccttcactgc cgtacgcata
    24601 gagagcgtgt aatcggtcat tttgccggta agtttcagtt tcagatcatc cgcctgatac
    24661 tgcttctcac cggtgaacgg ccagtaaagc tgtttgctgt tcacttccac gttgagtggc
    24721 aatccggctt ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg accggaaagg
    24781 ttaacgccaa tctccagctg ttcgcgcagc gcaccgccca ctttcagctt cagtttttct
    24841 cctttcaacg gctccacgtt tagtgtgcag ttgagggtga tatccaccgg ccagttgtct
    24901 gacagctgcg ccgtgccgct ggcgttgacg atcccttgac tggaatcgat atccagggcg
    24961 tccagtttag tattgccgtc aatgctgctc actttcagca gcatggtgcg cacggtgatg
    25021 tccgtgtcgc ccgtcacgcg taactgctcg cctttaaact cttcaatgtt caggttaagc
    25081 ggcaaatgca cgtcggtcat ttccggcaat accgggcgag aaaagagatc tttcagcgtt
    25141 tcgccgagcg gcttttcctc cggctgcgga ttttcaattt tcggttcgac cacttcttcc
    25201 tgcgccactt ccgccacttt cggcagagca atcagcaggc ctttcagcga cgtcggtttc
    25261 agggtcaggg ttttctcctg ccagttcagg ccggaggtga agtccatcac cgacaccgtg
    25321 gtgtcatcaa tcttgatgtt gacgttgtcc agcgccaccc gtgtcagggt gatgggatac
    25381 ggcgtggaga gatccagcgg accgctatct tcctcttctt caacctgttc agaaggaggc
    25441 atttttttac tgtcgatgtt gacctgaatg tctttcagcg ccaggtcatt aatacaaacg
    25501 ctactgttcc acaggcactc aagaccgacc gccagatgca gattacccgc ttttaccgcc
    25561 acgcctggct gctcataacg aacgtcagac aaggtgagat cgcgccagcc gccggtgacc
    25621 ttgccaatat ccagtcctgg cacccagcga tccgccgctt taaatacaag atgcaggccg
    25681 ctggtggtgc ccaccagaaa cgccaccgat cccagcaaca gtaagataac gataaccacg
    25741 ccgaggctga tttttttcca taaactcata attctggccc cagaccgatg taaaactgta
    25801 acccgtgttc gtctttatcc gcgaccggta cggcaaaatc gagtttgatt ggcccgaccg
    25861 gcgattccca gcgcacgccg accccggtac cggttttaaa gtcgctgcgg cgaatatcgc
    25921 ttaccgcttc gccactatcg acaaacaccg cgccccacca ttttccggtc acgttgtact
    25981 ggtattccag cgatccggtt atcaacttcg aggccccttt caggtcaccg ttggcgtatt
    26041 tcggggcgat agatttgtat ttgtagccgc gaatactgcg atcgcccccg gcgaagaaac
    26101 gcagatccgg cggtactttg tcgaaatcac cggtttcaat ccagcccagc gtgccgcgtg
    26161 taacgaaacg atggcgatcg tacagtgtgc ggatccagac gttctgcgcc tggaaaacgg
    26221 agaaatcgac atctgaaccc caggccgtgt tggagtagtc gatagagtag cgttgcgagt
    26281 cgccccaggt tggcatcagg ccaccacgag aacgcgtgcg gctaatcatc accccaggat
    26341 aaaacagcat cgtggtgttg gtaatttcac cctgggtaaa gtggtcgagg ctccagcgca
    26401 ggttaatggc acgctgccag ccgctggaga gatcccagta gcgagaagcc accagcgtag
    26461 tggagtcaga ttcggtatcg ttcaggtcag tgcgcttaaa accgccctgc accaaataat
    26521 attgttccag tggattcttc agcagcggca ttttatagct gaagtcgagg gtctgttccg
    26581 gcgcggaaat actggtactg gtggtcagac tgtgaccata agagttcatc cacggctttt
    26641 tccacgtcgc tttcacgcgc ggtcccacgt ccgtagagta accgaccccg gtttcgatgg
    26701 tgttttcagt tcgcggcgaa accacgcccg tcaagggtaa tactttcgtt tcgcgcgctt
    26761 tatcaaattg tggagccacc accaccgagt taaaccagcc ggtagcagaa agtcggcggt
    26821 tcagttccgc cagatctttc gattcgtact catcgccctc tttaaacggc accagatttt
    26881 gcaggtattc atcgcggatt tgtgatcctt caaaggtcac atgcccaaag cggtaacgtt
    26941 cgccactgtt ataatcaata tcccagaagg ctttatgcag gccgagcgca atgcccagct
    27001 gcgctttggt aaattcgcta tcgaaataac ctttacgcaa cgcaatgctg gttaaggact
    27061 ttttgaaatt ttcataatcg ccctggttca gcaccgtgcc aatagccggg cgagtatcga
    27121 gcaatttcaa atagtcttta tcggtccgcg cgccgccgcg caataccaca tcggtgccgc
    27181 caattaacac cggcacacct ggcgtgactt tggcgatcaa tacctgccgc cctttctttg
    27241 gcggtggacg gagatcaaat tcaatggtcg gctggtaata acccagcgct ttcagacctt
    27301 cgcggatggc atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact tcatcacttt
    27361 caatcgtaga aagctgcgca cgaacgttct tttccagctg tcccgataac ccctcgacct
    27421 gtagacggac gttcgcggcg acggcagatc cgcttaagca gagtaagctt acacagcata
    27481 actgtcggat atagcgcaca ttttctcctg aatatccttt tttcctgccc ctggaacgcc
    27541 gttaaacggc ttaacaaaaa tccagtaata tggattaaaa agcagactaa accccaaata
    27601 tttcttatgt tttactttag acctattcac ggtggttatt gtgtgcaaat acgcctcttg
    27661 ttacaacctt aaccccaatg accgattttc gggagagcga caccatgagt ttatttgata
    27721 aaaagcatct ggtttccccc gccgatgccc tgcctggacg taacaccccg atgcccgtag
    27781 ccacgctgca tgcggtcaac ggtcactcaa tgaccaatgt acctgacgga atggagattg
    27841 ccatttttgc gatgggttgt ttctggggag tggagcgtct gttctggcag ttacccggcg
    27901 tttacagcac cgccgcaggc tataccggag gctatacgcc aaatccgact tatcgggaag
    27961 tgtgctccgg tgatacgggt catgccgaag cggtacgcat tgtttacgat ccttccgtca
    28021 tcagctatga gcagttgcta caggtatttt gggagaatca cgatcccgcc cagggaatgc
    28081 gtcagggcaa tgaccacggc acgcagtatc gttcagcgat ttatccgctg accccagaac
    28141 aggatgccgc agctcgcgcc agtctggaac gttttcaggc ggcgatgctt gccgccgatg
    28201 acgatcgtca catcaccacg gaaatcgcta acgccacacc gttttattat gccgaagatg
    28261 accaccagca atatctgcat aaaaacccgt atggttactg tggaattggc ggaattggcg
    28321 tctgtctgcc accggaagca tagcgttacg ggtacaaatg tagattgttg ataaagtgct
    28381 ctttgtctat gccggatgcg gcgtgaatgc cttaccaggc ctacaaaatc gtccaaattc
    28441 aatatattgc agggactgcg taggcctgat cggcatagcg catcaggcag tattgcattt
    28501 atcagcagtc tgaattttaa ccctctggcg actttacagt accttacgct atactagcca
    28561 ctgaaaatgc cggttcactt tcttcgaatc ggctttcaat gtgtatttca cacaaattaa
    28621 tcaacttccc cttccgagga tctggcctga aaggtcggat aagatatgtt aaacagtatt
    28681 ttagtcatac tctgcttgat cgctgtaagt gcgttcttct cgatgtctga gatctcactt
    28741 gccgcctcac gcaaaatcaa acttaaactg ctggctgatg aaggcaatat aaatgcccaa
    28801 cgcgttctga atatgcagga aaatcccggc atgttcttta cggtggtcca aatcggtctg
    28861 aacgcagttg cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc agcttttcac
    28921 agcctgttct cccgctatat gtcggcagaa ctctctgagc aactgagctt tattctctct
    28981 ttctcgttag tgactggcat gtttatcctg tttgcggatt taaccccgaa acgcatcggt
    29041 atgattgcgc cagaagcggt ggctttgcgt atcatcaacc cgatgcgctt ctgcctgtac
    29101 gtttgcaccc cgctggtgtg gttcttcaac ggcctggcga acatgatctt ccgtattttc
    29161 aaactgccaa tggtacgtaa agatgacatc acttctgatg acatctacgc ggtagtggaa
    29221 gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg agctgattga aaacgtcttt
    29281 gagctggaat cccgtaccgt tccgtcttca atgacaccgc gtgaaaacgt gatttggttt
    29341 gatctccacg aagatgagca aagcctgaag aataaggtgg cggaacatcc gcactctaag
    29401 ttcctcgtct gtaatgaaga tattgaccac atcatcggtt atgtcgattc taaagacctg
    29461 ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca aattcgcaac
    29521 acgctgattg tgccggatac gttaaccctt tcagaggcgt tggaaagttt taaaaccgca
    29581 ggtgaagact tcgcggtgat catgaacgag tacgcgctgg tggtggggat catcaccctc
    29641 aacgacgtga tgaccacgct gatgggcgat ctggtcggtc aggggctgga agagcagatt
    29701 gtcgcccgtg atgagaactc atggctgatt gacggcggca ccccaattga cgacgtcatg
    29761 cgcgtgctgg atattgacga gttcccgcag tcgggcaact acgaaaccat cggaggcttt
    29821 atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt cgccggctac
    29881 aaatttgaag tggtggatat cgataactac cgcatcgacc agttgctggt gacccggatc
    29941 gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg ctaaagataa agaagaaagc
    30001 gtcgcgtaac ccccagaaac atcaacggct cctgaatcag gagccgtctt attactgcat
    30061 agcactttgg ttaagccatc tctgtttgca gacgcataac ctgacggtta acttcggaca
    30121 tcactgacaa atgcagctta tccttcactt ttgggataag aatcttaccc ttatcaaatt
    30181 caaaagcgcc aacgtccttg atgtataacc gtccacggaa caggatcttt acgtacttcg
    30241 ccacctgaag tgggttgtac cgttggaaaa ttttcattgt tatctcctgc tgagtattac
    30301 gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg caaattttaa tgcctagtga
    30361 ctatagacta tccgggcaat gtttccaccg cgtataactt tttttaccta aaggttacaa
    30421 ttattcagaa ttatcttttt accgaagcgc ggtcttcagt ataagcattc atttttcata
    30481 tgatttgtgc gcttgaccgc aaactggcat cacacttgcg ggaaattcga taaatagcac
    30541 atatgattaa aactcagacc caagtggtcg tatcacctgc atatcataag aaggaaacac
    30601 catgacccta cgcaagattc tggcactcac ctgcctgctg ttgccgatga tggcttccgc
    30661 acatcagttc gaaaccggtc agcgagtgcc gccgattggc atcaccgatc gtggcgagtt
    30721 ggtgcttgat aaagatcagt ttagctacaa aacctggaac agcgcgcagt tagtgggaaa
    30781 agtgcgagta ctgcaacata ttgctggtcg cacctctgca aaagagaaaa acgcgacgct
    30841 gattgaagcg attaaatcag cgaagttacc gcacgatcgt taccagacca ccaccattgt
    30901 taacaccgac gacgcaattc cgggttccgg catgtttgtg cgcagcagtc tggagagtaa
    30961 taaaaagctt tatccctggt cgcagtttat tgttgatagc aatggcgtcg cacgcggtgc
    31021 ctggcagctg gatgaaaaaa gttccgctgt agtggtgctg gataaagacg gtcgcgtgca
    31081 atgggccaaa gacggggcgc ttactcagga agaggtgcag caagtgatgg acctgctgca
    31141 taaattaatt aataaataga cactctgaac ccaggattca ggaacgactc gcgcggagtg
    31201 taatccagcg gtttaccctg ccagtcgtga acgtgcgctc cggcagctgc ggccacagca
    31261 tgtccagcgg cggtgtccca aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgt
    31321 ccttccgcca ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg atgttcgcca
    31381 agctgttgca gatactcttt cagctccgca tctgcatggg aacggctgat aaccaccagc
    31441 ggcgggcgcg catcgcggac ctgaatctgc ttgcgcacac cgcactcttc tttccaggcc
    31501 ttgccttctg ccgcgctgta cattacgttc attaccggcg catacaccac acctaaaatc
    31561 ggtttgccat ggtcaatgag cgcaatgtta acggtgaatt cgccattgcg tttaataaac
    31621 tctttagtac catccagcgg gtctaccagc cagtaacgct gccagtgctg acggacttcc
    31681 caaccgggag gatcttcttc agaaaggacc ggaatatccg gtgtcagcgt acgtaaaccg
    31741 tccatgataa cggtgtgtgc ggcaatatcc gctgccgtta ccggagaatt gtccgctttg
    31801 ctgacgacgt ccatcggttt cgtcccgtcg tagacctgca taatggcatc gcctgcattc
    31861 cgtgcaagct ggcatacttg atctaacatt tctccacctc gtctctgtga gcggtgttaa
    31921 cttattgttt tacttatacc ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat
    31981 tttcaaagcg taaaattgtg gcattcttca ctgttctata agtaagacgt ttattctccc
    32041 ttttctttcg tattcccgat gataaaagga tgtccctgat gattaagttt agcgcaacgc
    32101 tcctggccac gctgattgcc gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg
    32161 aaaccactga tctgcatagc aacatgatgg atttcgatta ttacaaagac accgccacgg
    32221 aaaaattcgg actggtacgt acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa
    32281 acagcgtact ggtcgataac ggcgatttga ttcaggggag tccgctggcc gattacatgt
    32341 cggcgaaagg attaaaagca ggtgatgttc atccggttta taaggcgctg aatacgctgg
    32401 attatacggt cggtacactc ggcaatcatg aatttaacta cggcctggat tacctgaaaa
    32461 atgcgctggc gggagcgaaa ttcccttatg taaatgccaa cgtcattgac gccagaacca
    32521 aacagccaat gtttacaccg tatttaatta aagataccga agtggtcgat aaagacggaa
    32581 aaaaacagac gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg
    32641 ataaagctaa tttatccgga aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat
    32701 acgtgcctga aatgcgcgag aaaggtgccg atgtcgttgt cgttctggcg cattccgggc
    32761 tgtctgccga tccgtataaa gtaatggcgg aaaactcagt ttattacctc agtgaaattc
    32821 cgggcgttaa cgccattatg tttggccatg ctcacgccgt tttcccgggt aaagattttg
    32881 ctgatatcga aggggctgat atcaccaaag gcacgctgaa tggtgttccg gcggtaatgc
    32941 cgggcatgtg gggcgatcat cttggtgtgg tcgacttaca actcagtaat gacagcggta
    33001 aatggcaggt gacgcaggcg aaagcggaag cacgaccgat ttacgacatc gctaataaaa
    33061 aatccctcgc ggcggaagac agcaagctgg tagaaacact caaagccgat cacgatgcca
    33121 cacgccagtt cgtcagcaag ccaatcggta aatctgccga caatatgtat agctatctgg
    33181 cactggtgca ggacgatccg accgtgcagg tggtgaacaa cgcgcaaaaa gcgtatgtcg
    33241 aacattacat tcagggcgat ccggatctgg caaaactgcc ggtgctttca gctgccgcac
    33301 cgtttaaagt tggtggtcgc aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc
    33361 agttgacctt ccgtaatgcc gccgatcttt atctctaccc caatacactg attgtggtga
    33421 aagccagcgg taaagaggtg aaagagtggc tggagtgctc tgccggacag tttaaccaga
    33481 ttgatcctaa cagcacgaaa ccacagtcac tcattaactg ggatggtttc cgcacttata
    33541 actttgatgt gattgatggt gtgaattatc agattgatgt tacccagccc gcccgttatg
    33601 acggcgagtg ccagatgatt aatgccaatg cggaaaggat taagaacctg acctttaacg
    33661 gcaagccgat tgatccgaac gccatgttcc tcgttgccac caataactat cgcgcttacg
    33721 gcggcaaatt tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc
    33781 gctcggtgct ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa attcacccgg
    33841 cggcagataa caactggcgt ttagcaccga ttgctggcga taagaaactg gatatccgtt
    33901 tcgaaacttc cccgtcagat aaagccgcag cgtttattaa agagaaaggg caatatccga
    33961 tgaataaagt cgcgaccgat gatatcgggt ttgcgattta tcaggtggat ttgagtaagt
    34021 aaaacacttc tttttcggcc tataaatcat caaccgcatc cggcatttat tggcggatgc
    34081 gatgctggcg catattatcc gccctacaag ccatgcaccg tagaccagat aagctcagcg
    34141 catccggcag ttatgccgca cgttcatccc gcaccgccaa cacctcgggc aaattcaact
    34201 caatccagtc cgccagtgca gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc
    34261 tgtattcaac atgcggtggc accaccggat acgcgatacg gttaagaaaa ccatcctgtt
    34321 ctaacgcctg taacgactgc gcaagcatct tttcgctcac cccgccgatt ttgcgccgca
    34381 ggtcgctaaa gcgatgagta ccttcgcgta gcgccaccag aatcaacacc ccccaacggc
    34441 tggtgacgtg tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga ttaccctctt
    34501 tcagttgttg cgacaggcta acctgactca tttcatactt acctttttgt acgtacttac
    34561 taaaagtaag tttaggtgtt agcgtattta aactcaagac aaaacgatgg agacttccca
    34621 tgatcgctat tactggtgcc actggccaac ttggtcacta tgttattgaa tccttgatga
    34681 aaacggttcc tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa gcccaggccc
    34741 tggcagcaca aggcattacc gtgcgtcagg ctgactacgg cgatgaagcc gcactgacat
    34801 ctgcacttca gggagtggaa aaactactgc tgatctcttc cagcgaagtg ggccagcgtg
    34861 ccccgcagca tcgcaatgtg attaacgcgg cgaaggcggc gggcgtgaaa tttatcgctt
    34921 ataccagtct gcttcacgcg gataaatctc cgctcggcct cgccgatgag cacatcgaga
    34981 cggagaaaat gctggctgat tctggcatcg tttacaccct gctgcgcaac ggctggtaca
    35041 gcgaaaacta cctcgccagc gcaccggcag cactggagca cggcgtattt atcggtgccg
    35101 cgggcgatgg caaaattgcc tcagcaacgc gggcagatta tgcggcagct gcggcacgcg
    35161 tgattagcga agccggtcac gaaggcaagg tttacgaact ggcgggcgat agtgcctgga
    35221 cgttgacaca gttagcggca gagctgacca aacagagcgg caaacaggtt acctatcaaa
    35281 atctgagcga agccgatttc gccgcggcac tgaaaagcgt cggactgccc gacggactgg
    35341 cggatatgct ggcggattct gacgttggcg catcgaaagg cggtctgttt gatgacagca
    35401 aaacgcttag caaattgatt ggccgcccaa cgacaacgtt agccgaaagc gtaagccatc
    35461 tttttaatgt taataactag ttaattaaag tggcatcctc ccgcatcctc tctgataatg
    35521 acgggatgcc gggagcaatc atgtctgctt cctgaacgtt tcttctgaca gaccaatgga
    35581 tgccagtaat gattagcggc gtgctgtacg ccctgttagc agggttgatg tgggggctta
    35641 tttttgtcgg gccgttgatc gtgccggaat acccggcgat gttgcagtcg atggggcgtt
    35701 atctggcgtt agggttaatt gcgctaccca ttgcctggct gggacgcgtg cgtctgcgtc
    35761 agttggcgcg tcgcgactgg cttaccgcct tgatgctcac tatgatgggc aacctcattt
    35821 attacttctg ccttgccagt gccattcaac gtactggcgc gcctgtttcc acgatgatta
    35881 tcggcaccct gccggtggtc attcctgtct ttgccaatct gctttatagc cagcgcgacg
    35941 gcaaactcgc gtggggaaaa ctcgccccgg cactgatttg tattggcatc ggcctcgcga
    36001 gtgtgaatat tgctgagtta aaccacggac tccccgattt tgactgggca cgttatacct
    36061 ctggcatcgt gctagcgtta gtttccgtgg tctgctgggc gtggtatgcc ctgcgcaacg
    36121 cccgctggct gcgggaaaat cccgacaaac atccgatgat gtgggcaaca gcgcaggcgc
    36181 tggtcacact gccggtttca cttatcggct atctcgtcgc ttgttactgg ctgaatacgc
    36241 aaacgccgga cttctcccta ccctttggcc cccgtccgct ggtgtttatt agtctgatga
    36301 ttgcgatagc cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc gccagccagc
    36361 gattaccgac agtgattctc gggccgctga ttgttttcga aacgctggca ggtttgctgt
    36421 acaccttttt actccgccag caaatgccgc cgctaatgac gctgagcggt atcgcgctgt
    36481 tagtgattgg cgtggtcatc gcggtcagag caaaaccaga aaaaccttta actgaatctg
    36541 tctcagaaag ttgacacgct ggcagtgagt taaataagcc tctgctacgt aagggttata
    36601 gcttttgcct taaagatgca tttaaaatac atcttatctt attaagaatg aggtatcagc
    36661 tatggcttat cgcgaccaac ctttaggtga actggcgctc tctattcctc gcgcttcagc
    36721 tctgtttcgt aaatatgata tggattactg ctgtggcggt aagcagacgc tggcgcgcgc
    36781 ggcggcacgt aaagaactgg atgttgaggt cattgaagct gaactggcaa agctcgctga
    36841 acaaccgatt gagaaagact ggcgtagcgc cccgctggca gaaatcattg accatatcat
    36901 cgtgcgctac cacgatcgtc accgcgagca gttgccggag ctgatcctgc aagcgactaa
    36961 agtcgagcgc gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa aatacctgac
    37021 tatgctgcat gaagagcttt ccagccacat gatgaaagaa gagcagatcc tcttcccgat
    37081 gatcaaacaa ggcatgggca gccaggcaat ggggccaatc agcgtaatgg aaagcgagca
    37141 cgatgaagcg ggtgaactgc tggaagtaat caaacacact accaataacg tcacaccgcc
    37201 gccggaagcc tgcaccacct ggaaagcgat gtataacggc attaatgaac tgattgatga
    37261 cctgatggat cacatcagcc tggaaaacaa tgtactgttc ccacgcgcgc tggcgggtga
    37321 gtgatctaaa agctggatgg cattgcgcca tccagcatga taatgcggtt atttccgcag
    37381 ttcagcagcc cgcttcttac caataaacag ccagcccaac cccagcgcga taaaccacag
    37441 cggagtaacc agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa
    37501 cacaaagaac gccatacata cccagcacat cagcttgccg agcggcatct tgtagatcga
    37561 cttctcatgc agatgaggac gctgtttgcg gtacaccagg tacgagcaaa ggataattgt
    37621 ccagacgaac ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac
    37681 gctaggattc acatacaaca tcaccacgcc gccgagcaga cagatacacg agaaggtcaa
    37741 ccctttcgcg ggtactgcgc gcttagaaag tttagcgaac gctttcggcg caacaccttc
    37801 ctgcgccaga ccaaacagca tacggctggt agagaagaca ccgctgttag cggaagacgc
    37861 cgcagaggtc agcaccacaa agttgatcac gctggcagcc gcaggcaaac ctaccaacac
    37921 aaacagctca acgaacgggc ttttctccgg gactaccgaa ctccacggcg tcacggacat
    37981 aatcacaatc agcgcgaaga cgtagaacat aatgatacgg atcggaatgg agttaatcgc
    38041 gcgtggcagt gatttctccg gatctttggt ttccgcagct gtagtaccta ccagctcaat
    38101 ccccacgaaa gcgaaaaccg ctatctggaa tccggcaaag aagccactta aacctttcgg
    38161 gaaccagccg ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg tcggtgactg
    38221 aaagtgcatc gccaccatga ccaggccgac gacaatcagc gacacgatgg cgacgatttt
    38281 gatcatcgca aaccagaact ccatctcacc gaacattttc acggtggcaa ggttgagcgt
    38341 cagcagcagc actatcaccg ccagcgaggc gacccagtcg gagagatcgg ggaaccagaa
    38401 ctgagcataa gccgtgatcg ccaccacgtc tgccataccg gttacaaccc agcagaacca
    38461 gtaggtccag ccggtgaaat atcctgccca cggcccgagt aaatcggaag cgaagtcact
    38521 aaaagatttg tattccagat tcgaaagcag caattccccc attgcccgca tcacgaaaaa
    38581 gagcataaaa ccaatgatca tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt
    38641 tttgccggac cccataaaca accccgtacc aatggcaccg ccaatggcaa taagctgaat
    38701 atgtcggttt gtgagattgc gccgtagcga ctgttcagcc ggagcctgat catcggcaac
    38761 gacttttacc tgatctacca tgtttttttc ttcctgtacc tgtctgtgtt gttcaggctc
    38821 tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc gatattaggt
    38881 aacaatacgc gggatgaata ctaagattta gatctaatgt taattttatg tttaaagtga
    38941 gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta cggggtcttt
    39001 gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg cctgctcccc
    39061 tctggttcga ggggaacaga atgcgctgct tagagaattt ccagcagttc gacttcaaac
    39121 accagggtac tgaacggagg gatggatgcg cctgcgccgc gctcgccata tgccagttcc
    39181 tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc ttcaatccag
    39241 ccagggatca cgccattaac cggaaattca gcgggttcac cacgagcaac ggagctgtca
    39301 aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc ggtacgtgcc
    39361 ggaattgcgc cttcaccctg gttgatcacg cggaattgca ggccagattc ggtgctattc
    39421 acaccttctt ttttggcgtt ttcttccagg tatttcacac cttcagcagc catcgcctgg
    39481 aaacgctgac gacgaacggc atcggcgcgc tcgtggattt cacgcagcgc gcgatgcacc
    39541 acatcaaccg gaacagccgg atgtttgcct tccagcgcat cggcaatacc tgcaaccagt
    39601 gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac ctgcaaacca
    39661 atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat cgtatttcct
    39721 ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg taaaactttg
    39781 tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc cgtatgaata
    39841 taataaaggc gggtttacca cggacatcat agcgttagct tattcatagg ctatgattga
    39901 ggaacaagac gcggagcagg aggaaaccat gcccgggcgc tttgaactaa aaccaaccct
    39961 ggagaaagtc tggcacgcgc cggacaattt tcgcttcatg gacccgctgc cgccgatgca
    40021 tcgtcggggg atcattattg ccgccattgt attggtggtc ggatttctgc ttccatctga
    40081 tgatacgccc aacgcgccgg tggtgacgcg tgaagcgcag ttagatattc agtcgcaatc
    40141 acaaccgccg acagaagaac agctgcgggc acaattagtc acaccacaaa atgatcctga
    40201 tcaggtggct ccggtcgctc cagaacctat acaggaaggc cagccagaag agcagccgca
    40261 aactacccaa acgcaaccat tccagccaga tagcggaatt gataatcaat ggcgctctta
    40321 ccgtgtcgaa cccggtaaaa cgatggcgca gctgttccgc gaccacggac tgcccgctac
    40381 cgatgtctat gcaatggcgc aggtagaagg cgcgggtaag ccgctgagta atttgcaaaa
    40441 cgggcaaatg gtgaaaatcc gccagaacgc cagcggcgtg gtgacgggct taaccattga
    40501 tacggggaat aatcaacagg tcctgtttac ccgccagccg gatggcagtt ttattcgtgc
    40561 gcggtagatg aagggtcaac caaaagccag ctaaggattg tctctttgat gaccttacgt
    40621 ctggcttcca gtgcctgggg ggaaaccata ctgatatgat aaaccaggcc aagcgtatgc
    40681 tggttgatca aataatatcc gccaagcgca gcaatattaa tattcacctg caatgggtcg
    40741 atatctggct taaaaatatt gtgtttcttc ccttcatcca ataaggattc cattaactgc
    40801 aagtgcgcat ggttaatttc taagagacgc tgtgattttg cataatgcac acctttactt
    40861 tgattttcac tatgcacaat ttttaaaaac caggggttag caatataata atccca
//
LOCUS       DAZFYC010000033        40309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFYC010000033 DAZFYC010000000
VERSION     DAZFYC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.033"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            276..362
                     /gene="leuL"
                     /locus_tag="ACHKW5_001930"
     CDS             276..362
                     /gene="leuL"
                     /locus_tag="ACHKW5_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO7511337.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            455..2026
                     /gene="leuA"
                     /locus_tag="ACHKW5_001931"
     CDS             455..2026
                     /gene="leuA"
                     /locus_tag="ACHKW5_001931"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO7511338.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2026..3117
                     /gene="leuB"
                     /locus_tag="ACHKW5_001932"
     CDS             2026..3117
                     /gene="leuB"
                     /locus_tag="ACHKW5_001932"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO7511339.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3120..4520
                     /gene="leuC"
                     /locus_tag="ACHKW5_001933"
     CDS             3120..4520
                     /gene="leuC"
                     /locus_tag="ACHKW5_001933"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO7511340.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4531..5136
                     /gene="leuD"
                     /locus_tag="ACHKW5_001934"
     CDS             4531..5136
                     /gene="leuD"
                     /locus_tag="ACHKW5_001934"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO7511341.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5185..6363)
                     /gene="setA"
                     /locus_tag="ACHKW5_001935"
     CDS             complement(5185..6363)
                     /gene="setA"
                     /locus_tag="ACHKW5_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO7511342.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIEQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6465..6596)
                     /gene="sgrT"
                     /locus_tag="ACHKW5_001936"
     CDS             complement(6465..6596)
                     /gene="sgrT"
                     /locus_tag="ACHKW5_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO7511343.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6685..8340
                     /gene="sgrR"
                     /locus_tag="ACHKW5_001937"
     CDS             6685..8340
                     /gene="sgrR"
                     /locus_tag="ACHKW5_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO7511344.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8374..8473
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8504..9487
                     /gene="thiB"
                     /locus_tag="ACHKW5_001938"
     CDS             8504..9487
                     /gene="thiB"
                     /locus_tag="ACHKW5_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO7511345.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9463..11073
                     /gene="thiP"
                     /locus_tag="ACHKW5_001939"
     CDS             9463..11073
                     /gene="thiP"
                     /locus_tag="ACHKW5_001939"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO7511346.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11057..11755
                     /gene="thiQ"
                     /locus_tag="ACHKW5_001940"
     CDS             11057..11755
                     /gene="thiQ"
                     /locus_tag="ACHKW5_001940"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO7511347.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11869..12633)
                     /gene="yabI"
                     /locus_tag="ACHKW5_001941"
     CDS             complement(11869..12633)
                     /gene="yabI"
                     /locus_tag="ACHKW5_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7511348.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12719..13597)
                     /gene="araC"
                     /locus_tag="ACHKW5_001942"
     CDS             complement(12719..13597)
                     /gene="araC"
                     /locus_tag="ACHKW5_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO7511349.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13936..15636
                     /gene="araB"
                     /locus_tag="ACHKW5_001943"
     CDS             13936..15636
                     /gene="araB"
                     /locus_tag="ACHKW5_001943"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO7511350.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPLLNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15647..17149
                     /gene="araA"
                     /locus_tag="ACHKW5_001944"
     CDS             15647..17149
                     /gene="araA"
                     /locus_tag="ACHKW5_001944"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO7511351.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17349..18044
                     /gene="araD"
                     /locus_tag="ACHKW5_001945"
     CDS             17349..18044
                     /gene="araD"
                     /locus_tag="ACHKW5_001945"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7511352.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18119..20470
                     /gene="polB"
                     /locus_tag="ACHKW5_001946"
     CDS             18119..20470
                     /gene="polB"
                     /locus_tag="ACHKW5_001946"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO7511353.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20635..23541
                     /gene="rapA"
                     /locus_tag="ACHKW5_001947"
     CDS             20635..23541
                     /gene="rapA"
                     /locus_tag="ACHKW5_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO7511354.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23553..24212
                     /gene="rluA"
                     /locus_tag="ACHKW5_001948"
     CDS             23553..24212
                     /gene="rluA"
                     /locus_tag="ACHKW5_001948"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO7511355.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24329..25144)
                     /gene="djlA"
                     /locus_tag="ACHKW5_001949"
     CDS             complement(24329..25144)
                     /gene="djlA"
                     /locus_tag="ACHKW5_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO7511356.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25399..27753
                     /gene="lptD"
                     /locus_tag="ACHKW5_001950"
     CDS             25399..27753
                     /gene="lptD"
                     /locus_tag="ACHKW5_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO7511357.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27806..29092
                     /gene="surA"
                     /locus_tag="ACHKW5_001951"
     CDS             27806..29092
                     /gene="surA"
                     /locus_tag="ACHKW5_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO7511358.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29092..30081
                     /gene="pdxA"
                     /locus_tag="ACHKW5_001952"
     CDS             29092..30081
                     /gene="pdxA"
                     /locus_tag="ACHKW5_001952"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO7511359.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNAPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30078..30899
                     /gene="rsmA"
                     /locus_tag="ACHKW5_001953"
     CDS             30078..30899
                     /gene="rsmA"
                     /locus_tag="ACHKW5_001953"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO7511360.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30902..31279
                     /gene="apaG"
                     /locus_tag="ACHKW5_001954"
     CDS             30902..31279
                     /gene="apaG"
                     /locus_tag="ACHKW5_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO7511361.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31286..32128
                     /gene="apaH"
                     /locus_tag="ACHKW5_001955"
     CDS             31286..32128
                     /gene="apaH"
                     /locus_tag="ACHKW5_001955"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO7511362.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32206..32685)
                     /gene="folA"
                     /locus_tag="ACHKW5_001956"
     CDS             complement(32206..32685)
                     /gene="folA"
                     /locus_tag="ACHKW5_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO7511363.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32877..34739)
                     /gene="kefC"
                     /locus_tag="ACHKW5_001957"
     CDS             complement(32877..34739)
                     /gene="kefC"
                     /locus_tag="ACHKW5_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO7511364.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGFIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34732..35262)
                     /gene="kefF"
                     /locus_tag="ACHKW5_001958"
     CDS             complement(34732..35262)
                     /gene="kefF"
                     /locus_tag="ACHKW5_001958"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO7511365.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35370..36701)
                     /gene="yaaU"
                     /locus_tag="ACHKW5_001959"
     CDS             complement(35370..36701)
                     /gene="yaaU"
                     /locus_tag="ACHKW5_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7511366.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36760..37047)
                     /gene="fixX"
                     /locus_tag="ACHKW5_001960"
     CDS             complement(36760..37047)
                     /gene="fixX"
                     /locus_tag="ACHKW5_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO7511367.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLESALEQWEYPRGTFGVEFRYG"
     gene            complement(37044..38330)
                     /gene="fixC"
                     /locus_tag="ACHKW5_001961"
     CDS             complement(37044..38330)
                     /gene="fixC"
                     /locus_tag="ACHKW5_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7511368.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38381..39322)
                     /gene="fixB"
                     /locus_tag="ACHKW5_001962"
     CDS             complement(38381..39322)
                     /gene="fixB"
                     /locus_tag="ACHKW5_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7511369.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39337..40107)
                     /gene="fixA"
                     /locus_tag="ACHKW5_001963"
     CDS             complement(39337..40107)
                     /gene="fixA"
                     /locus_tag="ACHKW5_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO7511370.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9679 a        11107 c        10604 g         8919 t
ORIGIN      
        1 caaatcatca ggataggttt tgcaacgcgt gcattttgtc ccctttttcc tcgttgatta
       61 gatgcaaaaa tttatgctga aatatgtcaa ccgatgaaaa gcgtcggtag ttaagcagaa
      121 attaatatcg cttactttaa ccaccgcagc acaattagct aattttacgg gtgcagaact
      181 cacgctggcg ggacgttttt attgcgtcag ggttgacatc cgtttttgta tccagtaact
      241 ctaaaagcat atcgcattca tctggagctg atttaatgac tcacatcgtt cgctttatcg
      301 gtctactact actaaacgca tcttctttgc gcggtagacg agtgagcggc atccagcatt
      361 aagccagcac gcagtcaaac aaaaaacccg cgccattgcg cgggtttttt tatgcccgaa
      421 gcgaggcgct ctaaaagaga caaggaccca aaccatgagc cagcaagtca ttattttcga
      481 taccacattg cgcgacggtg aacaggcgtt acaggcaagc ttgagtgtga aagaaaaact
      541 gcaaattgcg ctggcccttg agcgtatggg tgttgacgtg atggaagtcg gtttccccgt
      601 ctcttcgccg ggcgattttg aatcggtgca aaccatcgcc cgccaggtta aaaacagccg
      661 cgtatgtgcg ttagctcgct gcgtggaaaa agatatcgac gtggcggccg aatccctgaa
      721 agtcgccgaa gccttccgta ttcatacctt tattgctacc tccccaatgc acatcgccac
      781 caagctgcgc agcaccctgg acgaggtgat cgaacgcgct atctatatgg tgaaacgcgc
      841 ccgtaattac accgatgatg ttgaattttc ttgcgaagat gccgggcgta cacccattgc
      901 cgatctggcg cgagtggtcg aagcggcgat taatgccggt gccaccacca tcaacattcc
      961 ggacaccgtg ggctacacca tgccgtttga gttcgccgga atcatcagcg gcctgtatga
     1021 acgcgtacct aacatcgaca aagccattat ttccgtacat acccacgacg atttaggcct
     1081 ggcggtcggc aactcactgg cggcggtaca tgccggtgca cgccaggtgg aaggtgcaat
     1141 gaacgggatc ggcgagcgtg ccggtaactg ttcgctggaa gaagtcatca tggcgattaa
     1201 agttcgtaag gatattctca acgtccacac cgccattaat caccaggaaa tatggcgcac
     1261 cagccagtta gttagccaga tttgtaatat gccgatcccg gcaaacaaag ccattgttgg
     1321 cagcggcgca ttcgcacact cctccggtat ccaccaggat ggcgtgctga aaaaccgcga
     1381 aaactacgaa atcatgacac cagaatctat tggtctgaac caaatccagc tgaatctgac
     1441 ctctcgttcg gggcgtgcgg cggtgaaaca tcgcatggac gagatggggt ataaagaaag
     1501 tgaatataat ttagacaatt tgtacgacgc cttcctgaag ctggcggaca aaaaaggtca
     1561 ggtgtttgat tacgatctgg aggcgctggc cttcatcggt aagcagcaag aagagccgga
     1621 gcatttccgt ctggattact tcagcgtgca gtctggctct aacgatatcg ccaccgccgc
     1681 cgtcaaactg gcctgtggcg aagaagtcaa agcagaagcc gccaacggta acggtccggt
     1741 cgatgccgtc tatcaggcaa ttaaccgcat cactgactat aacgtcgaac tggtgaaata
     1801 cagcctgacc gccaaaggtc acggtaaaga tgcgctgggt caggtggata ttgtcgccaa
     1861 ctacaacggt cgccgcttcc acggcgtcgg cctggccacc gatattgtcg agtcctccgc
     1921 caaagccatg gtgcacgtac tgaacaatat ctggcgtgcc gcagaagtcg aaaaagagtt
     1981 gcaacgcaaa gctcaacaca acgaaaacaa caaggaaacc gtgtgatgtc gaagaattac
     2041 catattgccg tattgccggg ggacggtatt ggtccggaag tgatgaccca ggcgctgaaa
     2101 gtgctggatg ccgtgcgcaa ccgctttgcg atgcgcatca ccaccagcca ttacgatgta
     2161 ggcggcgcag ccattgataa ccacgggcaa ccactgccgc ctgcgacggt tgaaggttgt
     2221 gagcaagccg atgccgtgct gtttggctcg gtaggcggtc cgaaatggga acatttacca
     2281 ccagaccagc aaccagaacg cggcgcgctg ttgcctttgc gtaagcactt caaattattc
     2341 agcaacctgc gcccggcaaa actgtatcag gggctggaag cattctgtcc gctgcgtgca
     2401 gacattgccg ctaacggctt cgacatcctg tgcgtgcgcg aactgaccgg cggcatctat
     2461 ttcggtcagc caaaaggccg cgaaggtagc ggacaatatg aaaaagcgtt tgataccgag
     2521 gtgtatcacc gttttgagat cgagcgtatt gcccgcatcg cgtttgaatc tgctcgtaaa
     2581 cgtcgccaca aagtcacctc aatcgacaaa gctaacgtgc tgcaatcctc tattttatgg
     2641 cgtgaaatcg tcaacgagat cgccacggaa tacccggatg tcgaactggc gcatatgtac
     2701 atcgacaacg ccaccatgca gctgattaaa gatccatcac agtttgacgt tctgctgtgc
     2761 tccaacctgt ttggcgacat tctgtctgac gagtgcgcaa tgatcactgg ctcgatgggg
     2821 atgttgcctt ccgccagcct gaacgagcaa ggttttggtc tgtatgaacc ggcaggcggc
     2881 tcagcaccag atatcgcagg caaaaacatc gccaacccga ttgcgcaaat tctgtcgctg
     2941 gcactgctgc tgcgctacag cctggatgcc gatgatgcgg cttgcgccat tgaacgcgcc
     3001 attaaccgcg cattagaaga aggcattcgc accggggatt tagcccgtgg cgctgccgcc
     3061 gttagtaccg atgaaatggg cgatatcatt gcccgctatg tggcagaagg ggtgtaatca
     3121 tggctaaaac gttatacgaa aaattattcg acgctcacgt agtgtacgaa gccgaaaacg
     3181 aaaccccgct gttatatatc gaccgccacc tggtgcatga agtgacctca ccgcaggcgt
     3241 tcgatggtct gcgcgcccac ggtcgcccgg tacgtcagcc gggcaaaacc ttcgccacta
     3301 tggatcacaa cgtctctacc cagacgaaag acattaatgc ctgtggtgaa atggcgcgca
     3361 tccagatgca ggagctgatt aaaaactgca aagaatttgg cgtcgaactg tatgacctga
     3421 atcacccgta tcaggggatc gtccacgtaa tggggccgga acagggcgtc accttgccgg
     3481 ggatgaccat tgtctgcggc gactcgcata ccgccaccca cggcgcgttt ggcgcactgg
     3541 cctttggtat cggcacttcc gaagttgaac acgtactggc aacgcaaacc ctgaaacagg
     3601 gccgcgcaaa aaccatgaaa attgaagtcc agggcaaagc cgcgccgggc attaccgcaa
     3661 aagatatcgt gctggcaatt atcggtaaaa ccggtagcgc aggcggcacc gggcatgtgg
     3721 tggagttttg cggcgaagca atccgtgatt taagcatgga aggtcgtatg accctgtgca
     3781 atatggcaat cgaaatgggc gcaaaagccg gtctggttgc accggacgaa accaccttta
     3841 actatgtcaa aggccgtttg catgcgccga aaggcaaaga tttcgacgac gccgttgcct
     3901 actggaaaac cctgcaaacc gacgaaggcg caactttcga taccgttgtc actctgcaag
     3961 cagaagaaat ttcaccgcag gtcacctggg gcaccaatcc aggccaggtg atttccgtga
     4021 acgacaatat tcccgatccg gcttcgtttg ccgatccggt tgaacgcgcg tcggcagaaa
     4081 aagcgctggc ctatatgggg ctgaaaccgg gcattccgct gaccgaagtg gctatcgaca
     4141 aagtgtttat cggttcctgt accaactcgc gcattgaaga tttacgcgcg gcagcggaga
     4201 tcgccaaagg gcgaaaagtc gcgccaggcg tgcaggcact ggtggttccc ggctctggcc
     4261 cggtaaaagc ccaggcggaa gcggaaggtc tggataaaat ctttattgaa gccggttttg
     4321 aatggcgctt gcctggctgc tcaatgtgtc tggcgatgaa caacgaccgt ctgaatccgg
     4381 gcgaacgttg tgcatccacc agcaaccgta actttgaagg ccgccagggg cgcggcgggc
     4441 gcacgcatct ggtcagcccg gcaatggctg ccgctgctgc tgtgaccgga catttcgccg
     4501 acattcgcaa cattaaataa ggagcacacc atggcagaga aatttatcaa acacacaggc
     4561 ctggtggttc cgctggatgc cgccaatgtc gataccgatg caatcatccc gaaacagttt
     4621 ttgcagaaag tgacccgtac gggttttggc gcgcatctgt ttaacgactg gcgttttctc
     4681 gatgaaaaag gccaacagcc aaacccggac ttcgtgctga acttcccgca gtatcagggc
     4741 gcttccattt tgctggcacg agaaaacttc ggctgtggct cctcgcgtga gcacgcgccc
     4801 tgggcattga ccgactacgg ttttaaagtg gtgattgcgc cgagttttgc tgacatcttc
     4861 tacggcaata gctttaacaa ccagctgctg ccggtgaaat taagcgatgc agaagtggac
     4921 gaactgtttg cgctggtgaa agccaatccg gggatccatt tcgacgtgga tctggaagcg
     4981 caagaggtga aagcgggaga gaaaacctat cgctttacca tcgatgcctt ccgccgccac
     5041 tgcatgatga acggtctgga cagtattggg cttaccttgc agcacgacga cgccattgcc
     5101 gcttatgaag caaaacaacc tgcgtttatg aattaatccc cttgcccggt caaatgaccg
     5161 ggctttccgc tatcgtccac gtcatcaaac gtctttaacc tttgcggtta aaaataatgc
     5221 gacaacagaa ataaccgcaa ttacccagta gacagcaaag tgcccccaac tttgttcaat
     5281 tgctccctga ataacgccag ccagaattac cccggtagaa atactgttag taaataaggt
     5341 ggtagctgcc cccgctcttc caggcattaa atcctgaaac catagcatcc caatacccgc
     5401 aacaatgccg ataaatacag cgttaaaaag ttgcagcgtc atcaacgcca tacggctatg
     5461 aaagaaaatc aatccggtgt aaaacagtac tcctgccgcc actgctatga ccatcattcg
     5521 ccgcttacca taacgtttga catagtagcc agccagaatc attgctggta tttccagtcc
     5581 agctgccgtc cccatcagga aacccgccag tttgtctggc aatcctaact cgctactgat
     5641 ccacaacggc atatcaataa tgtacatggt gttgcaggtc cacattaacg tcgaggcgac
     5701 aaataacatc cgtacgttac tatcctgcca gccaccttgc attgataaag cattttccga
     5761 cggcagttct acccgcgcca cagacggaag cataaatgca atcaatacca gactgagtgt
     5821 gaatatcccg gcggcaatcg aaaacatcac cgtaaagccg taattcaacg ccagcataaa
     5881 ggccaacggt ggaccgataa cccatgccag agaaagctgc gcacgcatca ccgagctaaa
     5941 catcaccact tctcgcgccg agttatccgc atattcccgc gccagagcaa ataactgtgg
     6001 cattgccgta ttggccagag atgccagaag cacaccacag gtgataagcg tcagataatg
     6061 acgattaaat gcaaacaata gcgcattgcc gatagccatc aaacagcaaa atataatcag
     6121 ttttcgccga tcgccctgac tgtcagaacg ttttgccaac cagaggctta cgccgatccc
     6181 agcaatagca ttcaccgtat aaaagagtcc gatccagaaa ggttgcgcgc caacctcacg
     6241 actcagaaat aagctcaatg taggagcctg tagcgccccg gccaccccca tcataaaagc
     6301 gaccagcata aatgccgcgt aaacaccgtt catacggcga gccatcgtca ttatccagat
     6361 catacgttcc ctttttagcg cggcgagaat aaaaaaaacc agcaggtata atctgctggc
     6421 gggtgatttt acaccaatac tcagtcacac atgatgcagg caagtcaact ttcagaattg
     6481 cggtcatccc actgcatcag ttcttcaagc atggttaatc gttgtgggac gcttaaccaa
     6541 cgcaaccagc acaacttcgc tgtcgcggta aaatagtgct gataaaactg acgcatgggg
     6601 cacctccttg cttcatcgtt aggaattatt gacttaatat agggaaaata aaattgctgt
     6661 cttttgcgca ggagttcccc ttttatgcca tctgctcgtc tgcaacaaca gttcatccgc
     6721 ctgtggcaat gctgcgaggg taaatcgcag gacacaacgc tcaacgaact ggcagcgtta
     6781 ttgagctgct cgcgtcgtca tatgcgtacc ctgctcaaca ccatgcagga tcgcggctgg
     6841 ctgacgtggg aagcggaagt cgggcgcggt aaacgctcgc gtctgacatt cctctatacc
     6901 gggctggcgc ttcagcaaca gcgggcggaa gacctgctgg agcaggatcg tatcgaccaa
     6961 ctagtgcagt tggttggcga caaagcgact gtgcggcaaa tgctggtttc tcatctgggc
     7021 cgcagcttcc gccaggggcg gcacatcctg cgcgtgctct actatcgtcc gttgcgtaat
     7081 ctgctacctg gcagcgcatt gcgccgttcc gaaacccata ttgcccggca aatcttcagt
     7141 tcgctaacgc gcataaatga ggaaaatggg gaactggaag cagacatcgc ccaccactgg
     7201 cagcaaatat caccgcttca ctggcgtttc tttttgcgtc caggagtcca ttttcatcat
     7261 ggtcgtgaac tggaaatgga cgacgtgatc gcctctttaa aacgaatcaa tacgctgccg
     7321 ctctattcgc atattgcgga cattgtgtcg ccgacgccct ggacgctgga tatccatctc
     7381 acgcaaccgg accgctggtt accgttactg ctgggacaag ttccggcgat gatcctgccg
     7441 cgcgaatggg aaaccctcag taactttgcc agccatccca tcggcaccgg tccgtatgcg
     7501 gtgatccgca acaccaccaa tcaactgaaa attcaggcat tcgatgactt cttcggttac
     7561 cgggcattaa tcgatgaagt taacgtctgg gttctgccgg aaattgccga cgagccagct
     7621 ggaggtctga tgctaaaagg tccacagggt gaggaaaaag agattgaaag ccgcctggag
     7681 gaaggttgct actatttact gttcgacagc cgcacccatc gcggggcgaa tcagcaagtc
     7741 agggactggg taagctatgt gctttctcca actaatctgg tctatttcgc tgaggaacag
     7801 taccagcaac tgtggttccc ggcttatgga ctgctccccc gttggcacca tgcccgcacc
     7861 ataaagagcg aaaaaccggc tggcctggaa agcctcatcc tgacctttta tcaggatcac
     7921 agtgagcatc gggtgattgc cgggatcatg cagcagattc tggcaagtca ccaggtcacg
     7981 ctggaaatca aagagatcag ctacgatcag tggcatgaag gagagatcga gagcgatatc
     8041 tggcttaaca gcgccaactt tacgctgccg ctggattttt cgctgttcgc gcacttgtgc
     8101 gaggtgccgc tgctccaaca ctgtattccg atcgactggc aagccgacgc cgcccgctgg
     8161 cgcaatggcg aaatgaacct ggcgaactgg tgccagcaac tggtcgccag taaagcaatg
     8221 gtgccgctta tccaccactg gctgatcatt cagggacaac gcagtatgcg cggcctgcgt
     8281 atgaataccc tcggctggtt cgattttaaa tcagcgtggt ttgcgccgcc ggatccatga
     8341 ttgctggtag ataacaaaat cactacacta acgccgttct caacggggtg ccacgcaaac
     8401 gcgtgcgctg agaaaatacc cgtcgaacct gatccggata acgccggcga agggatttga
     8461 ggctccttct caagtccttt gccactcttt tttgaggtgc aaagtgttaa aaaaatgtct
     8521 tcccctgctg ttgctgtgca cagcgcccgt tttcgctaaa cccgttctga ctgtttatac
     8581 ctacgattcc ttcgccgccg actgggggcc tggtccaaag atcaaaaaag cttttgaagc
     8641 cgactgtaac tgcgaactga aactggtggc gctggaagat ggcgtttcgc ttctcaaccg
     8701 tctacggatg gaaggcaaaa atagtaaagc cgatgtggtg ctggggctgg ataacaatct
     8761 gttagacgcc gccagtaaaa ccggactatt tgccaaaagc ggtgtggcag cggatgccgt
     8821 taacgttccc ggcggctgga ataatgacac tttcgtcccg tttgattacg gctatttcgc
     8881 cttcgtctat gacaagaaca aactgaaaaa cccgccgcaa agcctgaaag aattggttga
     8941 gagcgatcag aactggcggg tgatttatca ggatccgcgc accagtacgc cggggctggg
     9001 cctgctgttg tggatgcaaa aagtctatgg cgatgacgcc ccacaagcct ggcaaaaact
     9061 ggcgaagaaa accgtcacgg tcaccaaagg ctggagcgaa gcctacggcc tgtttttaaa
     9121 aggtgaaagc gatctggtac tgagttacac cacctctccg gcttatcaca ttctcgaaga
     9181 gaaaaaagat aactacgccg ccgcgaactt tagcgaaggt cactatctgc aggtggaagt
     9241 cgccgcccgc accgctgcca gcaagcagcc ggagctggcg caaaaattcc tccagtttat
     9301 ggtttctccg gcttttcaga atgcgatccc aaccggtaac tggatgtatc cggtggcaaa
     9361 tgtcacgctg cctgccgggt ttgaacaatt gaccaaaccg gccaccacgc tggagttcac
     9421 accagctgaa gtggcggcac aacgtcaggc atggattagc gaatggcaac gcgccgtcag
     9481 ccgttaattc ccggctggtt aattccaggt gtaagcgccg ccacgctggt ggtagcggtt
     9541 gcgctcgcgg cgtttctcgc cctgtggtgg aacgcgccgc agggtaactg ggtggcagtc
     9601 tggcaggaca gctacctgtg gcatgtggtg cgcttctcct tctggcaggc gtttctctcg
     9661 gcgctactct ctgtcgtacc cgcgatattc ctcgcccgcg ccctctatcg caggcgcttt
     9721 ccgggtcggc tggcgctgtt gcgtctgtgc gcaatgacct tgatcctccc ggtgctagtt
     9781 gctgttttcg gcattcttag cgtctatggt cgtcagggct ggctggcatc gctctgccaa
     9841 tcgctcggtc tggagtggac cttttcgccc tacggcctgc aaggcatttt gctggcgcac
     9901 gtatttttta atctgccgat ggcgagccgc ttattactcc aggcactgga aaacattccc
     9961 ggcgaacaac gtcagcttgc cgcccagctt gggatgcgcg gctggcattt tttccgcttc
    10021 gtcgaatggc cgtggctacg gcgacaaatc ccgccggttg ccgcgcttat ctttatgctc
    10081 tgtttcgcca gcttcgccac cgtgctatcg ctgggcggcg gtccgcaggc aactactatc
    10141 gagctggcta tttatcaggc gctgagttac gactacgatc ctgcccgcgc ggcgatgctg
    10201 gcgctgctcc agatggtgtg ttgccttggg ctggtgctgt tgagtcagcg actaagtaag
    10261 gccattgcgc ccggtaccac gctgctgcaa ggctggcgcg atccggacga tcgtctgcat
    10321 agccgcattt gcgacacggt gttaatagtg ctggcgctgc tgctgttgct gccaccgttg
    10381 ctggcggtga tcgtcgatgg ggtaaatcgc cagttgccgg aagtgctggc acaaccggtg
    10441 ctgtggcagg cgctgtggac ctcgttgcgt attgcgctgg cggcaggtgt attgtgcgta
    10501 gtgctgacca tgatgctgct atggagcagt cgcgaactgc gggcgcggca gaaaatgctg
    10561 gcgggtcagg cgctggagat gagcggcatg ttgatcctcg ccatgccggg gattgtgctg
    10621 gctaccggct tctttttact gctcaacaac actatcggcc tgccacaatc tgctgacggc
    10681 attgtgattt tcaccaatgc gttaatggcg atcccttatg cgctgaaagt actggaaaac
    10741 ccgatgcgcg atatcaccgc ccgctacagt atgttgtgtc agtcgctggg cattgaaggc
    10801 tggtcgcgct taaaagtggt cgagctgcgc gccctgaaac gtccactggc gcaggcgctg
    10861 gcttttgcct gcgtgctgtc gattggtgat tttggcgtag tggcgttgtt cggtaacgat
    10921 gatttccgta ccctgccgtt ttatctctac cagcaaattg gctcctatcg cagccaggac
    10981 ggcgcggtca ccgcgttaat tctgctacta ctctgttttc tgctgtttac cgtgattgaa
    11041 aaactaccgg ggcgaaatgt taaaactgac tgatatcacc tggctttatc accatttgcc
    11101 gatgcgtttt agcttaacgg tagaacgcgg cgagcaggtg gcaatcctcg ggccaagcgg
    11161 cgcgggtaaa agtaccctgc tgaatttgat tgccggtttt ctgacgccag ccagcggttc
    11221 gctgactatc gacagcgttg atcacaccac gacgccgccg tcacgccgtc cggtatcgat
    11281 gctgtttcag gagaacaacc tgttcagcca cctgacggtc gcgcagaaca tcgggctggg
    11341 gcttaatccg ggattgaagc tgaacgcggc gcagcaggag aaaatgcacg ctatcgcccg
    11401 ccagatgggg attgataatt taatggcgcg gttaccgggc gagctttccg gcggtcagcg
    11461 acagcgcgtg gcgttagcgc gttgtctggt gcgcgaacag ccgattttat tgctcgatga
    11521 accgttctct gcgctcgatc cggcgttacg tcaggagatg ctgacgctgg tgagcacgag
    11581 ctgccagcag caaaaaatga cgctattgat ggtgtcgcac agtgtggaag atgcggcgcg
    11641 gatcgccaca cgctcggtgg tggtcgccga cgggcgcatc gcctggcagg gtaagaccaa
    11701 tgagttgttg agcggtaagg cgagtgcttc ggcactattg gggattacgg gttagtatgc
    11761 actgtcggat gcggcgtgaa cgccttatcc gacctacggg tagtgcgttt tgtaggcctg
    11821 ataagacgcg gtcagcgtcg catcaggcaa cccgcacaag actatctcct aacccccaac
    11881 cactttacgc aaaatatcga tatacaccgg catcaacggg tggcgaatta acaccaccag
    11941 cgccaccacg ccgatggcag aaattaacgg cgtcagccac aacaaacggc cgcgcgacaa
    12001 ataatgactt aaacgatcgg ttgctttacc gctgcgccat aaccgccagc acagccagcc
    12061 accaacccac aaaaataccg ccgtcgccag cagcaaccat ttaaactcgc cgctctgcat
    12121 tccggctgga atatcgatcg ccgcgcccgc cagaatccca ggcaggaagt aaaacggtgg
    12181 ccacagcagg cagccgataa tattcggcgt aatgaatttt gccaccggca gatccagcat
    12241 tcccgccacc attggcacca gcggacgcgt cgggccaaca aagcgaccga ccagaatggt
    12301 gaacatgctg tgttgatgca acgcatgttc agttttatcg agtaatgctt tgtttttctt
    12361 cagaaatgac cagcgatgca acggcttttt aaaacgccag cccagccaga aggagatcca
    12421 gtcgcccagc agacaaccga caatccctgc cagccaggcg tgccagaaac ttaactcgcc
    12481 gctgccaatc agcgctccca gccccgccat cagcaccgta ccgggtagaa tcaaaccgac
    12541 cagcgccagc gactccagaa aggccaccaa caccaccgcc atcaatgaat acacggtgga
    12601 ttgggtaata aagtgttcca gcaatgcttg cataatgtgc ctgtcaaatg gacgaagcag
    12661 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt
    12721 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
    12781 ggctggcccc ggtgcatttt ttaaatactc gcgagaaata gagttgatcg tcaaaaccaa
    12841 cattgcgacc gacggtggcg ataggcatcc gggtagtgct caaaagcagc ttcgcctgac
    12901 taatgcgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
    12961 gtgacagacg cgacggcgac aggcaaacat gctgtgcgac gctggcgata tcaaaattgc
    13021 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacacgatta tccatcggtg
    13081 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
    13141 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
    13201 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaacccgta ttggcaaata
    13261 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
    13321 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
    13381 gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg atttttcacc accccctgac
    13441 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
    13501 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggcg ttaaacgagt
    13561 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
    13621 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc
    13681 tcttctcgct aacccaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga
    13741 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca
    13801 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag
    13861 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt
    13921 ggatggagtg aaacgatggc gattgcaatt ggcctcgatt ttggcagtga ttctgtgcga
    13981 gctttggcgg tggactgcgc cagcggtgaa gagatcgcca ccagcgtaga gtggtatccc
    14041 cgttggcaaa aagggcaatt ttgtgatgcc ccgaataacc agttccgtca tcatccgcgt
    14101 gactacattg agtcaatgga agcggcactg aaaaccgtgc ttgcagagct tagcgtcgaa
    14161 cagcgcgcag ctgtggtcgg gattggcgtt gacagtaccg gctcgacgcc cgcaccgatt
    14221 gatgccgacg gtaacgtgct ggcgctgcgc ccggagtttg ccgaaaaccc gaacgcgatg
    14281 ttcgtattgt ggaaagacca cactgcggtt gaagaagcgg aagagattac ccgtttgtgc
    14341 cacgcgccgg gcaacgttga ctactcccgc tatattggcg gtatttattc cagcgaatgg
    14401 ttctgggcaa aaatcctgca tgtgactcgc caggacagcg ccgtggcgca atctgccgca
    14461 tcgtggattg agctgtgcga ctgggtgcca gctctgcttt ccggtaccac ccgcccgcag
    14521 gatattcgtc gcggacgttg cagcgccggg cataaatctc tgtggcacga aagctggggc
    14581 ggcctgccgc cagccagttt ctttgatgag ctggacccgc tcctcaatcg ccatttgcct
    14641 tccccgctgt tcactgacac ctggactgcc gatattccgg tgggcacctt atgcccggaa
    14701 tgggcgcagc gtctcggcct gcctgaaagc gtggtgattt ccggcggcgc gtttgactgc
    14761 catatgggcg cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact
    14821 tccacctgcg acattctgat tgccgacaaa cagagcgttg gcgagcgggc agtgaaaggt
    14881 atttgcggtc aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa
    14941 tcggcgtttg gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag
    15001 cttgccgccc agcatccgga actgaaagcg caaatcaacg ccagccagaa acaactgctt
    15061 ccggcgctga ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc
    15121 gactggttta acggccgccg cacaccgaac gctaaccaac gcctgaaagg ggtgattacc
    15181 gatcttaacc tcgctaccga cgctccgctg ctgttcggcg gtttgattgc tgccaccgcc
    15241 tttggcgcac gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg
    15301 atggcgctgg gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg
    15361 ctgaatcgcc cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt
    15421 tttgctgccg tcgccgcgaa agtgcacgca gacatcccct cagcccagca aaaaatggcc
    15481 agtgcggtag agaaaaccct gcaaccgtgc agcgaacagg cacaacgctt tgaacagctt
    15541 tatcgccgct atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc
    15601 ccggcacagg ctgcccaggc cgttccgact ctataaggac acgataatga cgatttttga
    15661 taattatgaa gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg
    15721 tcaggtcacc caacatgccg agcacgtcgt taatgcgctg aatacggaag cgaaactgcc
    15781 ctgcaaactg gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg
    15841 cgacgcgaat tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc
    15901 ggccaaaatg tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac
    15961 ccagtttaac gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca
    16021 gactgctcat ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc
    16081 cgtcgttacc ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg
    16141 tcaggcggtc tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat
    16201 gcgtgaagtg gcggtcaccg atggtgataa agttgccgca cagatcaagt tcggtttctc
    16261 cgtcaatacc tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga
    16321 tgttaacgcg ctggtcgatg agtacgaaag ctgctacacc atgacgcctg caacacaaat
    16381 ccacggcgaa aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg
    16441 tttcctggaa caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct
    16501 gaaacagctt ccaggtctgg ccgtacagcg tctgatgcag cagggttacg gctttgcggg
    16561 cgaaggcgac tggaaaactg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct
    16621 gcagggcggt acctccttta tggaggacta cacctatcac ttcgagaaag gcaatgacct
    16681 ggtgctcggc tcccatatgc tggaagtctg cccgtcgatt gcagtagaag agaaaccgat
    16741 cctcgacgtt cagcatctcg gtattggtgg taaggacgat cctgcccgcc tgatcttcaa
    16801 tacccaaacc ggtccagcga ttgttgccag cctgattgat ctcggcgatc gttaccgtct
    16861 gctggttaac tgtatcgaca cggtgaaaac cccgcactcc ctgccgaaac tgccggtggc
    16921 gaatgcgctg tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc
    16981 tggtggcgcg caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt
    17041 cgccgagatg cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt
    17101 taaagacgca ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc
    17161 aggtatgtaa cgcctgatgc gacgctgacg cgtcttatct ggcctacgcg ctgtgttttt
    17221 gtaggccgga taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct
    17281 tatcaggcct acgcgctgcg attttgtaga ccggataagc aaagtgcatc cggcacgaag
    17341 gagtcaacat gttagaagat ctcaaacgcc tggtattaga agccaacctg gcgctgccaa
    17401 aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg
    17461 tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg
    17521 tggttagcat cgcaaccggt gaagtggttg aaggtacgaa aaagccctcc tctgacacgc
    17581 caactcaccg gctgctctat caggcattcc cgtccattgg cggcattgtg catacgcact
    17641 cgcgccacgc caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc
    17701 acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca
    17761 acggtgaata tgagtgggaa accggtaacg tcatcgtaga aaccttcgaa aaacagggta
    17821 tcgatgcagc gcaaatgccc ggcgtcctgg tccattctca cggcccattt gcatggggca
    17881 aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga
    17941 tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac
    18001 actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca
    18061 cagccaatca aacgaaacca ggctataatc aagcctggtt ttttattgga ttttcaacgt
    18121 ggcgcaggca ggttttatct taacccgaca ctggcgggat accccgcaag ggacagaagt
    18181 ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc
    18241 cgtggcgttt attcctgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca
    18301 aggctttcgc ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct
    18361 ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt
    18421 taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac
    18481 ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa
    18541 accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg
    18601 ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct
    18661 ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg
    18721 cccgctgctg ctggaaaaac tcaacgcctg gtttgcaaac cacgatcccg atgtgatcat
    18781 cggctggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg
    18841 tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa
    18901 aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct
    18961 gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt
    19021 aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg accgccgttt
    19081 cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca
    19141 gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct
    19201 gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat
    19261 gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccaccgcacg ccagccctgg
    19321 cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa
    19381 aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg
    19441 catggcgcag cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg
    19501 ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa
    19561 acgtcagggt aataaaccac tttcgcaggc gctgaagatc attatgaatg ccttttatgg
    19621 cgtgcttggc accaccgcct gccgcttttt cgatccgcgc ctggcatcgt cgatcaccat
    19681 gcgtggtcat cagatcatgc ggcaaaccaa agcattgatt gaagcgcagg gctacgacgt
    19741 tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga
    19801 ggcggcgaaa atcggtcgtg tactggtgca gcacgttaac gcctggtggg cggaaacgct
    19861 gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt
    19921 tctgatgcca accattcgcg gagccgatac cggcagtaaa aagcgctacg ccgggctgat
    19981 tcaggagggc gataaacagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg
    20041 gacgccgctg gcccagcagt ttcagcagga gctatacctg cgcatcttcc gcaacgagcc
    20101 atatcaggaa tatgtacgcg aaaccatcga caaactgatg gcgggtgaac tggatgcgcg
    20161 actggtttac cgtaaacgcc ttcgccgtcc gctgagcgag tatcagcgta atgtgccgcc
    20221 tcatgtacgc gccgctcgcc ttgccgatga agaaaaccaa aagcgcggtc gccccttgca
    20281 atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga
    20341 ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc
    20401 ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg
    20461 gctattttga gcaaaaaaaa gagttcgcca gataccattt tgatgcgtga cgaatgcttt
    20521 gccatccagt accatagcgc cctttccatt cctggacctg aataacacca ctacctcata
    20581 agcacggtag cgggtggtta ttgcctgcaa ttaaagatat agagccgaac acatatgcct
    20641 tttacacttg gtcaacgctg gatcagcgat acagaaagcg aattgggact tggaaccgtt
    20701 gtcgcggtgg atgcgcgaac tgtcacttta cttttcccat ctactggtga aaaccgtctg
    20761 tacgcacgca gtgattcccc cgtgacccgc gtgatgttca accctggtga tactattacc
    20821 agccatgacg gctggcagat gcaagtcgaa gaagtaaaag aagaaaatgg cttgctgacc
    20881 tatatcggta ctcgcctgga tactgaagag tccggcgtag ccctgcgtga agttttcctt
    20941 gatagcaaac tggtgttcag caaaccgcag gaccgtctgt ttgccgggca gattgaccgt
    21001 atggaccgct ttgcgctgcg ttatcgcgcg cgtaagtatt ccagcgaaca gttccgtatg
    21061 ccgtacagcg gcctgcgcgg tcagcgtacc agcctgattc cgcatcagct caacatcgct
    21121 catgatgttg gtcgccgcca cgcgccgcgc gtcctgctgg ctgacgaagt gggtttaggg
    21181 aaaaccattg aagccgggat gatcctgcat cagcaactgc tctctggcgc tgctgaacgt
    21241 gtgctgatta tcgtcccgga aaccttacag catcagtggc tggtagaaat gctgcgccgt
    21301 ttcaacctgc gctttgcact gtttgatgat gagcgttatg ccgaagctca gcacgatgct
    21361 tacaacccgt ttgacaccga acagctggtg atttgctcgc tggattttgc ccgtcgtagc
    21421 aaacagcgcc tggaacatct ctgtgaagcc gaatgggacc tgctggtggt cgatgaagcg
    21481 catcacctgg tgtggagcga agatgcgcca agccgtgaat atcaggccat tgaacaactg
    21541 gcagagcacg tgccgggcgt tctgctgctg accgcgaccc cggaacagct ggggatggaa
    21601 agccacttcg cccgtctgcg tctgctggac ccgaaccgtt tccacgattt tgcgcagttc
    21661 gttgaagagc agaaaaatta tcgtccggtt gcggacgccg ttgccatgct gctggcaggt
    21721 aacaaactga gcaatgacga actgaacatg ctcggcgaga tgatcggcga gcaggatatc
    21781 gagccgctgt tgcaggcagc aaacagcgac agcgaagatg cccagagcgc ccgtcaggag
    21841 ctggtttcga tgctgatgga tcgccacggc accagccgcg tgctgttccg taacacgcgt
    21901 aacggtgtga aaggattccc gaaacgcgag ctgcacacca ttaaactgcc gctaccgacg
    21961 cagtatcaga cggctattaa agtctccggc attatgggcg cacgtaaaag tgcggaagat
    22021 cgtgctcgcg atatgctcta cccggagcgt atttatcagg aatttgaagg tgataacgcc
    22081 acctggtgga acttcgatcc gcgcgttgag tggctgatgg gctacctgac cagccatcgc
    22141 tctcagaaag tgctggtgat ctgcgccaaa gctgccactg cgctgcaact ggagcaggta
    22201 ctgcgcgaac gtgaaggtat tcgcgctgcg gtgttccacg aaggtatgtc gattatcgaa
    22261 cgtgaccgcg ctgccgcctg gtttgccgaa gaagacaccg gcgcacaggt actgctgtgc
    22321 tcagaaatcg gttctgaagg acgtaacttc cagttcgcca gccacatggt gatgtttgac
    22381 ctgccattca acccggatct actggagcag cgtattggtc gtctggatcg tatcggccag
    22441 gcgcacgata ttcagatcca tgtgccttat ctggagaaaa ccgctcagtc ggtgctggtg
    22501 cgctggtatc acgaaggtct ggatgcattt gagcacacct gcccgaccgg acgcactatt
    22561 tacgatagcg tatacaacga tctgattaac tatctggctt caccggatca aaccgaaggc
    22621 tttgacgatc tgatcaaaaa ctgccgcgag caacatgaag cgctgaaagc acagctggaa
    22681 cagggtcgtg accgcctgct ggaaatccac tccaacggtg gcgaaaaagc ccaggcactg
    22741 gcagaaagca ttgaagagca ggatgacgat accaacctga tcgccttcgc catgaacctg
    22801 ttcgatatta tcggtatcaa tcaggacgat cgcggcgaca acatgatcgt gctgacgccg
    22861 tccgatcata tgctggtgcc ggacttccct ggcctgtcgg aagatggcat caccatcacc
    22921 tttgatcgtg aagtggcact ggcgcgtgaa gatgcacagt ttattacctg ggagcatccg
    22981 ctgatccgca acggtctgga tctgatcctt tctggcgata ccggtagcag cacgatttca
    23041 ctgttaaaaa acaaagcgtt gccggtaggt acgctgttgg tggaactgat ttatgtggtt
    23101 gaagcccagg ctccgaagca gttgcagctc aaccgcttcc tgccaccgac gccggtacgt
    23161 atgctgctgg ataaaaacgg caacaacctg gcggcgcagg tagagtttga aacctttaac
    23221 cgccagctta acgcggttaa ccgtcacacc ggcagcaaac tggttaacgc cgtgcagcag
    23281 gatgttcacg ctatccttca actgggtgaa gcgcagatcg agaaatctgc ccgtgcattg
    23341 attgatgcag cgcgtaacga agccgacgaa aaactgtctg ccgagctgtc tcgtctggaa
    23401 gctctgcgtg cagtgaaccc gaacattcgt gacgacgaac tgaccgccat tgagagcaac
    23461 cgtcagcagg taatggaaag cctggatcag gcaggctggc gtctggatgc cctgcgtttg
    23521 atcgttgtaa cgcatcagta acggagccga aaatggggat ggaaaactac aatccaccgc
    23581 aggaaccctg gttggttatc ctgtatcagg atgaccacat tatggtggtt aacaagccga
    23641 gcggtttgtt gtctgtgccg ggtcgtctgg aagagcacaa agatagcgtg atgacgcgca
    23701 ttcagcgtga ttatccgcag gcagaatcgg tgcatcgtct ggatatggct accagcggcg
    23761 tgattgtagt ggcgctgacc aaagccgcgg agcgggagtt aaaacgccag ttccgcgagc
    23821 gcgagccgaa aaagcagtat gtggcccgcg tctgggggca tccatccccc gcagaaggtc
    23881 tggtggatct gccgctgatt tgcgactggc caaaccgccc gaaacagaaa gtctgttacg
    23941 aaacgggtaa acctgcgcag acggaatatg aagtggtgga gtatgcggcg gataacacgg
    24001 caagagtggt gttaaaaccg attaccgggc gttcgcatca attgcgtgtg catatgctgg
    24061 cgctgggtca tccgattctc ggcgatcgtt tttatgcatc accagaagcg agagcgatgg
    24121 caccacgttt gttgctacat gcagagatgt tgacgattac ccatccggcg tatggcaata
    24181 gtatgacgtt taaagcgcca gcggattttt aatcgtcacg ctggcgcagt tcggtaggcg
    24241 gataaggcgt tcacgccgca tccggcaatt gtgcgccgat tgcctgatgc gacgcgggcc
    24301 gcgtcttatc aggcctacag tttaaaactt atttaaaccc tttctgctgc tttatcagct
    24361 catatgcctg ctgaatttcc tgcgctttct gcttcgccat ctccatcatc tcaggcggca
    24421 aacctttcgc caccagctta tcgggatggt gttcactcat cagcttacgg taggcacgtt
    24481 tgatggtggt cgcatcatcc gtcggcttca cgcccagcac attacaggca tcttccagcg
    24541 tcgggccacg ctgcgcttgc tgccagttac caccgccaga ttgctgctga taaccgccgc
    24601 caaactgtgc accgccctgc atcatgcgca aaaactggtc aaactgggcg cgggagatcc
    24661 ctaattcttc agcaatgaca tacagcaccg cccgttcatt cgggtgcagt gaaccatcag
    24721 caaacgccgc ctgaatctgg atctccagaa acatacgaat taagtcaaaa cgaccaaagc
    24781 agacactgcg aaactggcgc atcttttcgc gcagcgggta attatctgat tttcccactc
    24841 ggaacgcgtt ttgcgccgca gtacgggaag cgccatgaag gttcattcgg tccatcaact
    24901 ggctggcgat atgaatatca gcctccgtga cgcgaccttt ggatttggtt aaatgcccca
    24961 tcacttcaaa agtggtggca aaaaacagcg cctgacgctc acgctggttg gcgaaccacg
    25021 ccattttacg gctacgggct ttatcaaaca tatggccaat taacaggcct aacactacgc
    25081 cccaaaagcc gccgcccatc agtaaggcca cggccacgcc aatgattttt ccccaatact
    25141 gcatatattc cccaaatcga cacacggata tcagggctat ctcccacaat ataaaggtgc
    25201 ttttaccgtt ttccggctgc ggtcaattgt gggacatcgc ctataatttg cattatcata
    25261 cctgtcattc aatgccgtgc ctaacaccac tgacgctatt cggacaggat taacactagc
    25321 gtagagatga cgagtacgtt agtctctgac cgtttgtcac gcgcaacgtt accgatgatg
    25381 gaacaataaa atcaacgtat gaaaaaacgt atccccactc tcctggccac catgattgcc
    25441 accgcccttt atagtcaaca gggactggca gccgacctcg cctcacagtg catgttgggc
    25501 gtgccaagct atgaccgtcc tctggtacag ggcgatacca atgacttacc cgtgactatc
    25561 aatgctgacc acgcgaaagg ggactacccg gatgacgccg tgtttactgg cagcgtggat
    25621 atcatgcagg gtaacagccg tctgcaggcc gacgaagtgc agctccatca aaaagaggca
    25681 ccaggacaac cggagccggt acgtaccgtt gatgcgctcg gtaatgtcca ttacgacgat
    25741 aaccaggtga tcctcaaagg gccgaaaggc tgggcgaatc tgaacaccaa agataccaac
    25801 gtctgggaag gtgattacca gatggtgggt cgccagggtc gcggtaaagc ggacctgatg
    25861 aaacaacgtg gcgaaaaccg ctataccatt ctggataacg gtagctttac ctcctgtctg
    25921 ccgggttctg acacctggag cgtggtaggt agcgaaatta ttcatgaccg cgaagaacaa
    25981 gttgcggaga tctggaacgc ccgctttaag gtgggtccgg taccgatctt ttatagcccc
    26041 tatttgcagt tgccggtggg tgacaaacgt cgctctggtt tcttgatccc gaacgccaag
    26101 tacaccacca ccaactactt tgagttctac ctgccatatt actggaacat cgcgccaaat
    26161 atggatgcca ccatcacgcc gcattatatg catcgtcgtg gcaacatcat gtgggagaac
    26221 gaattccgct acctctccca ggcgggcgct ggcttgatgg aactggacta tctgccttca
    26281 gataaagtct atgaagatga acacccgaac gatgacagtt cacgtcgttg gttattctac
    26341 tggaaccact ccggggtcat ggatcaggtg tggcgtttca acgtcgacta caccaaggtc
    26401 agcgatccta gctacttcaa tgatttcgat aacaagtacg gttccagtac tgacggctac
    26461 gcaacgcaaa aattcagcgt tggctatgcg gtgcaaaact tcaatgccac cgtttcaacc
    26521 aagcagttcc aggttttcag cgaacagaac accagtagct actcggcaga gccgcagtta
    26581 gacgttaatt actaccagaa tgatgttggt ccgtttgata cgcgtattta cggccaggca
    26641 gtgcactttg ttaacaccag agacgacatg cctgaagcaa cccgtgttca cctggaaccg
    26701 accatcaatt tgccgctctc taataactgg ggcagcatca ataccgaagc gaagttgctg
    26761 gcaacccatt atcagcaaac caatcttgac tggtataact ccagaaacac gaccaagctg
    26821 gacgaatccg ttaaccgcgt aatgccgcaa ttcaaagttg acggcaaaat ggtctttgaa
    26881 cgcgatatgg aaatgctggc tccgggttat acccaaacgc tggaaccgcg cgcgcagtat
    26941 ttgtacgtgc cgtatcgcga tcagagcgac atctataact acgactcgtc tctgctgcaa
    27001 tctgactact ctggcctgtt ccgggaccgg acttacggcg gtcttgaccg tattgcctcc
    27061 gctaaccagg tgacgaccgg tgtcacatct cgcatatatg atgatgctgc cgttgaacgt
    27121 tttaatattt ccgttggtca aatctactat ttcacggagt ctcgcactgg cgatgacaac
    27181 ataacatggg agaatgacga caaaacgggt tcactggtgt gggcaggcga tacttactgg
    27241 cgtatctccg agcgttgggg attgcgtggc gggattcagt acgatacacg tctggataac
    27301 gtagcgacca gtaactccag cattgaatac cgtcgggatg aagaccgtct ggtacagctg
    27361 aattaccgtt acgccagccc ggaatatatt caggctacgc tgcctaagta ctattccact
    27421 gctgagcaat ataagaatgg tatttcgcag gtaggtgctg tcgccagctg gccaattgcc
    27481 gatcgttggt ccattgttgg ggcctactac tacgacacca atgcgaacaa gcaagccgac
    27541 tctatgttag gtgtgcaata cagctcctgc tgctatgcaa ttcgcgtcgg ttacgagcgg
    27601 aagctgaacg gttgggataa cgataaacaa catgcggtat atgacaacgc aatcggcttt
    27661 aacatcgaac ttcgcggcct gagctccaac tacggtctgg gtacgcaaga gatgctgcgt
    27721 tcgaacattc tgccgtatca aaacactttg tgatttgttg atttaccacg taatccgcag
    27781 tgcggttaat tgaaatggaa aaagtatgaa gaactggaaa acgctgcttc tcggtatcgc
    27841 catgatcgcg aataccagtt tcgctgcccc ccaggtagtc gataaagtcg cagccgtcgt
    27901 caataacggc gtcgtgctgg aaagcgacgt tgatggatta atgcagtcgg taaaactgaa
    27961 cgctgctcag gcaaggcagc aacttcctga tgacgcgacg ctgcgccacc aaatcatgga
    28021 acgtttgatc atggatcaaa tcatcctgca gatggggcag aaaatgggag tgaaaatctc
    28081 cgatgagcag ctggatcagg cgattgctaa catcgcgaaa cagaacaaca tgacgctgga
    28141 tcagatgcgc agccgtctgg cttacgatgg actgaactac aacacctatc gtaaccagat
    28201 ccgcaaagag atgattatct ctgaagtgcg taacaacgag gtgcgtcgtc gcatcaccat
    28261 cctgccgcag gaagtcgaat ccctggcgca gcaggtgggt aaccaaaacg acgccagcac
    28321 tgagctgaac ctgagccaca tcctgatccc gctgccggaa aacccgacct ctgatcaggt
    28381 gaacgaagcg gaaagccagg cgcgcgccat tgtcgatcag gcgcgtaacg gcgctgattt
    28441 cggtaagctg gcgattgctc attctgccga ccagcaggcg ctgaacggcg gccagatggg
    28501 ctggggccgt attcaggagt tgcccgggat cttcgcccag gcattaagca ccgcgaagaa
    28561 aggcgacatt gttggcccga ttcgttccgg cgttggcttc catattctta aagttaacga
    28621 cctgcgcggc gaaagcaaaa atatctcggt gaccgaagtt catgctcgcc atattctgct
    28681 gaaaccgtcg ccgatcatga ctgacgaaca ggcccgtgtg aaactggaac agattgctgc
    28741 tgatatcaag agtggtaaaa cgacttttgc tgccgcagcg aaagagttct ctcaggatcc
    28801 aggctctgct aaccagggcg gtgatctcgg ctgggctaca ccagatattt tcgatccggc
    28861 cttccgtgac gccctgactc gcctgaacaa aggtcaaatg agtgcaccgg ttcactcttc
    28921 attcggctgg catttaatcg aactgctgga tacccgtaat gtcgataaaa ccgacgctgc
    28981 gcagaaagat cgtgcatacc gcatgctgat gaaccgtaag ttctcggaag aagcagcaag
    29041 ctggatgcag gaacaacgtg ccagcgccta cgttaaaatc ctgagcaact aatggttaaa
    29101 acccaacgtg ttgtgatcac tcccggcgag cccgccggga ttggcccgga cttagttgtc
    29161 cagcttgcac agcgtgagtg gccggtcgaa ctggttgttt gtgccgatgc cactctcctt
    29221 accgaccggg cagcgatgct cggtttgccg ctcaccctcc gcccttattc ccccaacgcc
    29281 cctgcacaac cgcaaactac gggcacatta acgctacttc ctgtcgcgct acgtgaatct
    29341 gtcactgcgg ggcagttagc gattgaaaat ggacattacg tggtggagac gctggcgcga
    29401 gcgtgtgatg gctgtctgaa cggtgaattt gctgcgctga tcacaggccc cgtgcataaa
    29461 ggcgtcatta acgacgcagg cattccgttt accggtcata ccgagttttt cgaagagcgt
    29521 tcgcaggcga aaaaagtggt gatgatgctg gcgaccgaag aacttcgcgt ggcgctggca
    29581 acgacgcatt taccgctgcg cgatatcgca gatgctatca cccctgcact tttgcacgaa
    29641 gtgattgcta ttttgcatca cgatttgcgt acaaaatttg gtattgccga accgcgcatt
    29701 ctggtctgcg ggctgaatcc gcacgcgggc gaaggcggtc atatgggtac ggaagagata
    29761 gacaccatta ttccggtgct cgacgagctg cgggcgcagg gaatgaaact caacgggccg
    29821 ctgcctgccg ataccctgtt tcagccgaaa tatctcgata acgccgacgc cgtgctggcg
    29881 atgtaccacg atcagggtct tcccgtgcta aaataccagg gcttcgggcg cggtgtgaac
    29941 attacgctgg gcctgccctt tattcgcaca tcagtggacc acggcaccgc gcttgaactg
    30001 gcgggacgtg gcgaagccga tgtcggcagt tttattacgg cgcttaatct cgccatcaaa
    30061 atgattgtta acacccaatg aataatcgag tccaccaggg ccacttagcc cgtaaacgct
    30121 tcgggcaaaa ctttctcaac gatcagttcg tgatcgacag cattgtttct gccattaacc
    30181 cgcaaaaggg ccaggcaatg gtcgaaatcg gccccggtct ggcggcattg accgagccgg
    30241 tcggcgaacg tctggaccag ctgacggtca tcgaacttga ccgcgatctg gcggcacgtc
    30301 tgcaaacgca tccattctta ggcccgaaac tgacgattta tcagcaggat gcgatgacct
    30361 ttaactttgg cgaactggcc gagaaaatgg gtcagccgct gcgtgttttc ggcaacctgc
    30421 cttataacat ctccacgccg ttgatgttcc atctgtttag ctatactgat gccattgccg
    30481 acatgcactt tatgttgcaa aaagaggtgg tgaatcgtct ggttgcagga ccgaacagca
    30541 aagcgtatgg tcgattaagc gtcatggcgc aatactattg caacgtgatc ccggtactgg
    30601 aagttccgcc gtcagccttt acaccaccac ccaaagtgga ttccgccgtc gtgcgcctgg
    30661 ttcctcatgc aacgatgcct cacccggtta aagatgttcg tgtgttgagc cgcatcacca
    30721 ccgaagcctt taaccagcgt cgtaaaacca ttcgtaacag cctcggcaac ctgtttagcg
    30781 tcgaggtgtt aacgggaatg gggatcgacc cggcgatgcg agcggaaaat atctctgtcg
    30841 cgcaatattg ccagatggcg aactatctgg cggagaacgc gcctttgcag gagagttaac
    30901 gatgatcaat tcgccccgag tgtgtattca ggttcaaagc gtctacattg aggcacaatc
    30961 ttcacctgat aatgaacgtt acgtttttgc ttataccgta accatacgca atctgggacg
    31021 agcgccagtg cagttgttgg ggcgttactg gctgatcacc aatggcaatg gccgtgaaac
    31081 cgaagtccag ggcgaaggag tggttggcgt ccagccactt atcgcgcctg gcgaagagta
    31141 ccagtacacc agcggtgcaa tcattgaaac cccgctgggc accatgcagg gtcactacga
    31201 aatgatcgat gaaaatggcg tccctttcag catcgacatt cccgtattcc gactcgccgt
    31261 tcccacactc attcattaaa agaatatggc gacatacctt attggcgacg ttcatggttg
    31321 ttacgatgaa ctgatcgcat tgctgcataa agtagaattt acccctggga aagataccct
    31381 ctggctgacg ggtgatctgg tcgcgcgcgg gccgggttcg ctggatgttc tgcgctatgt
    31441 gaaatcctta ggcgacagcg tacgtctggt gctgggcaat cacgatctgc atctgctggc
    31501 ggtatttgcc gggatcagcc gcaataaacc gaaagatcgc ctgacaccgc tgctggaagc
    31561 gccggatgcc gacgagctgc ttaactggct gcgtcgccag cctttgctgc aaatcgacga
    31621 agagaaaaag ttggtgatgg cccacgcagg gatcacgccg cagtgggatc tacagaccgc
    31681 caaagagtgc gcgcgcgatg tagaagcggt gctatcgagt gactcctatc ccttctttct
    31741 cgacgctatg tacggcgata tgccaaataa ctggtcaccg gaattgcggg ggctgggaag
    31801 actgcgtttt atcaccaacg cctttacccg tatgcgtttt tgctttccaa acggtcaact
    31861 ggatatgtac agcaaagaat cgccggaaga agcccctgcc ccactgaaac cgtggtttgc
    31921 gattcctggc cctgtcgctg aagaatacag catcgccttt ggtcactggg catcgctgga
    31981 aggcaaaggt acgccggaag gtatttacgc gctggatacc ggctgctgct ggggtgggtc
    32041 attaacctgc ctgcgctggg aagataagca gtattttgtc cagccgtcga accggcataa
    32101 ggatttgggc gaagcggcgg cgtcttaaac acagcctgat ataggaaggc cggataagac
    32161 gcgaccggcg tcgcatccgg cactagccgt aaattctata caaaattacc gccgctccag
    32221 aatctcaaag caatagctgt gagagttctg cgcatcggcg tcgtggaatt cgctgaatac
    32281 cgattcccag tcatccggct cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc
    32341 gatatgcgtc agatacagtt tttgcgcttt tggcaggaac tgctcataaa cgcgaccgcc
    32401 gccaatcacc atgatttctg gtacgtcacc acacgccgcg atggcttcat ccaccgactt
    32461 cacccacgtt acgcgatcgt ccgtacccgg ttgactgctg aggataatat ttttgcgtcc
    32521 tggcaacgga cgaccgattg attcccaggt atggcggccc ataatcacgg gtttatttaa
    32581 ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc cacggcatgg cgttttccat
    32641 gccgataacg cgatctaccg ctaacgccgc aatcagactg atcattgaga tttcccgata
    32701 aaaaaaattg tcgccactat acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat
    32761 gttgaggaaa attttatatt ctgctggcga gtccacgctc tctccctgga ctcgccgcat
    32821 tacaatgaaa caaaaacaaa cagttagctg taaagtgtga tttacgtcac tctttattag
    32881 gatgagggtt tcgtttccgg ttcatccgcc atgttgccgg tatgtttacc ttcttcggtt
    32941 ccctgccagc catgtcgttg aattaatgac agatgttcgc gatcctcggt aatgatctca
    33001 cttaacatcg cgctggtgcg tttatagacc gccgcgcggg ctttggtatc attctcaacc
    33061 attgccatct cttccaccat ctgaatatta aagcggcgga acacatcggc acgttctcgc
    33121 gcttcatacg gccccagacc taaactttcc agtgccagac gcccggtttt cagcgcacct
    33181 tcgaaggttt cacgctccgg cttttcaacg cctgcctgac gcaaacgaat gtagtggtcg
    33241 acatcgcggg cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac catctctgtc
    33301 agttgcaggt tggtttgcgg atcgtcgatg gcgttaatca gcacttccgc tttcgccgct
    33361 ccggcagatt ccagtaaatc catccgcgtg gcatcgccat aaaacacttt cataccaaat
    33421 ttacgcaagg tttcgatatg gtccggatcg tgatcgagta ccaccatttt caccccgctg
    33481 gagagcagta aacgtccggt aatctgccca aaacgaccga atccggcgat aatcacgcgc
    33541 ggctgttctt cgtcgatctc atcggcttca cgcgcttcct cagtagaaga ttgctcaagg
    33601 cgattgagga tcaccagcag aatcggcgtt gctgccatcg acagcgccac cgccagggtc
    33661 agcgatttcg cccactccgg ctccagcaca ttcgccatct gcgccgcgcc aaataccaca
    33721 aaggcaaact cactgccctg ccctaacaac accgcaaacc aacgacgctg tttatttggc
    33781 acttgcaacg gtcgggcaat cagccacagc atggcgattt tgatgatgag gaaaccgagc
    33841 agcaaaatga caatgcgcaa tgggttttca agcagcgtgc caaagtctat cgacatgcca
    33901 acaccgatga aaaacagccc caacagcaaa cctttaaatg gttcgatatc gctctccagc
    33961 gcatgacggt attccgagct tgccagcagt acgcccgcca gaaacgcgcc catcgccatc
    34021 gacaagccga cctcttccag cagcaaacca aagccaaaca cgaggaataa cgccacggca
    34081 ctaaacactt cccgcaagcc agagcgggct acaaaacgca gcgccggacg cgtgacatag
    34141 cgccccagca ataccaccag caccagcgca cccgccactt ttaacgccga gagagcaaat
    34201 gcgcccatcg tcgtcgaggc actgctcgtt gccagtagcg gaatcatcgc caccagcggg
    34261 atcgccgcga tatcctggaa cagcagcacc gcaaaggcac tgcgacccat ttgcgtcacc
    34321 atcagattgc gttcattcat cgcctgcatg gcaatcgccg tagaggagag cgccagcgtc
    34381 atgccgatca attccgcgac ctgccagcgc agcccaagta acatgcagaa cagccccagc
    34441 aggccgccgc aaatcaccat ctgcaatgcg ccaccgccga acactgccgc acgcagcttc
    34501 cacagccttt gtggatcgag ttcgaggccg ataataaaca gcatcagcac cacgccaatc
    34561 tcggcaaagt gcagaataga ttcggcatcg gtcaccagtc gcagccccca cgggccaata
    34621 atgaagccgg cgatcaggta gccaagtacc gatcccagac caagacgtac cgcaatgggt
    34681 acaatcagcg ctgccgaacc gagataaatc agcgcctgaa tcagcgtatg gctatccatg
    34741 atgggcctcc tgccattcca gcagacgttg cttatagtga cgcgcctgcc cttcgagggt
    34801 ttcgtcgtca caaataaagg tgcagtgcat ggcaaacggt ggcagccagt tcagcccgca
    34861 gtagattgcc gtcgcctgta gcggctgcga cagcacatca aagcccggat gcgcaccaat
    34921 ttcaaaatgg ctttccccgc cgccggtcgt caccgcccac agcaaatgtt tgccatgcag
    34981 cgccgtgccg ccgtgaccgt aagcccagcc gtgcgagaaa actttatcga tccaaagttt
    35041 gaggagcggt ggaatgctgt accactgcat cgggtgctgc cagacgatca gatcggcgcg
    35101 agacagcgcc tcctgctcgg cggcaatatc gatattgaag tcaggataga gttgataaag
    35161 agagcgaatt tcgacgcctt ccagcgtcct tgcctgttca agcatccgtt tattcgcatg
    35221 ggaatgatgc ggatacggat gcgcataaat tataagaatc ataggtagcc cgcgtgatta
    35281 cgcctttgtt ttcccacaaa gtgtagtcat atattcacgc ggcttatagt caataatatt
    35341 gattcagaaa agagagaaat ctgaacaatt taccccattc tctgcccgcg gatcgtcata
    35401 ttgctggttt gcgccagtga catccctcga gtctccgggg caaacgctac ggaaatcaac
    35461 aagccaaaca gcgagatacc cgcccccatt agcatcgtgt tactgatacc gtaattattg
    35521 ataaagatcg gcagtgccca ggtcgaaaca atggtgccaa tacggcttaa ggacataatc
    35581 acgcccacgg cagaggcgcg gatatctgtc gggaagagtt cattaggata gagccactgc
    35641 aaattacccg gcccgccaga gaaaaaggca tacaccgcaa aggccatcac taccagccag
    35701 atccccatat ccgggatcag ccccaaaacc gccagcgcca gcgtcatcat ggcaaagctg
    35761 ccaatcaaca atggacgccg tccggcagtg tttaaccaca gcatcggcgg aatacagccg
    35821 agcataaaga acaggctaat caccacgttc cccagcgccg cgtttttgcc aacacccaat
    35881 cccaacaaac caacgatttg cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg
    35941 caggtccaga tggcggcaac aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg
    36001 cgaaaacggg tttgctgcgg ttgctcttca tcgaaagcca ccggttcgcc aaacagtttt
    36061 atcatcatct cttcgcactc ttttactcgc cctttgcgta ataaccagcg gggagattca
    36121 ggcagttcga atcgaccaat caaaatcaac aaacagggga tcgccgcgct acccagcatc
    36181 cagcgccagc cgccttccac atcataaagc cagtagccga ccagatcggc gcaggtcgcg
    36241 ccgacatacc acatggcggc gataaagctg atggaaaacg cccgctgacg ggtactggag
    36301 aactcggtga tcattgaggt ggcgatggga taatctgcac cgatgacaat gccgataagt
    36361 acccgcatca ccaacagttc gacgggggat gaaacaaaca tcgtcgccac cgatatcacg
    36421 ccgatggcga tgatatcaat gaggaacatt ttgcgccgtc cgactttatc ggaaatatag
    36481 ccaaacagcg atgtgccaac gaacagcccg gcgagcgttc ccgcgcccaa caagccaatc
    36541 cagtcagcgt ccagttttag cgccggagtc agttgctcca gcgccacgcc aatcattacc
    36601 agtacataac catccagaaa cggaccaccg cttccccaca gcaaaatgcg gcggtgaatt
    36661 gaggagaatt tgagatcgtc aaagtttctg gacggttgca tgggttattc ctgttttttt
    36721 gcaatgagag tcagtacggc gttacgtatc aaaccaacat cagccgtaac ggaactccac
    36781 accaaaggtg ccgcgcgggt attcccactg ttccagcgcc gactccagcc ccagaatgcg
    36841 acaggtgccg cactccagac atccggcgta atcgaagcgc acactgccgt catcctgctt
    36901 cttgtacaga cctgcggggc acgctttcac cagcagctcc agggcctgtt tatcggcatc
    36961 ggccttcaca acaatgtgcg gatgctcttc atcgacattg aatttattga cgcccagttt
    37021 gacgtccaca ttgacgggag aagtcataaa acggtcactc ctttcatgcc atccttgatc
    37081 agattgatga agcccacttt cttgccgtgg cggaggattt tcttgcgcat cagttccggc
    37141 gcactgccgt caatggtgaa caggtcacgc gccacgccca ccgccagttc cgggtagccg
    37201 ctaaacatgc gtgggttatc aaggaaggcc ggtagtttct ggtacatacg catatcgcgc
    37261 agcgggccac tctcaagatg ctgacgatat tccgccagtt tttgcttact gaaatcgtcg
    37321 cttttcatcg ctgaaagcac ggtttttgct gcggcttccc cggcggcaat cgccagatcc
    37381 ataccgcgaa tggtaaaacc gaggttcata cacattccgg cggcatcacc ggcaatcaat
    37441 acgccgtcac caaccaactc cggcagcatg ttgatgcctg cttccggcac tacgtgagcg
    37501 gaatattcca ccagcttgcc acccgcgatc agcggtgcaa cggccggatg ttgtttgaaa
    37561 tcttccagca tttgcggcac cgattttttt gcgtcatgca gatgatgcag accacaaacc
    37621 agccccagcg acagggtatt ttcattggta taaaggaagc cgccgcccat caggccatcg
    37681 gtgggtgatc ccgcaaacag acaagccgcg ccctgattac cctgcaactg aaaacggtct
    37741 tcgattaccg acttcggtaa ctcgatcagt tccttcacgc caaccgccac atccgtcggt
    37801 ttaacgcgtt ttgccatccc cagcttttcg gcaaggatgg agttcactcc atcagcaagg
    37861 atcaccgttt tcgcttcaat cacatcgcca tcggcttcta caccgacgac tttgccatcg
    37921 cgctgtacga ggttatcgac gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc
    37981 tgctccatca gccaggcatc aaatttactg cgcaaaacgg agtaagaacg ctgggatggc
    38041 gaggtttcgt caccattgca gtagtccata gtcatcgctg acttttccgt cataaacgcg
    38101 agtttttcat gggtgatcag gcgttctacg ggggcggagt cggcgaaacc aggaataatg
    38161 tgttccaggc tgtgggcata gagacgcccg ccggtgacgt tcttggcacc tgcggaattg
    38221 ccacgctcga taactaacac ttgcgcccct tcgcgggcga gcaccagtgc ggcaaccgaa
    38281 ccggcaagcc cagcaccgac gatgatggcg tcaaagatat cttcggacat aagagctccc
    38341 tgtcagcggc agggccaaaa tgcagccctg ccagagtgga tcaacgcgct aaagctgcgg
    38401 tcagcgccgg aaggatcttc acggcgtcac caacaatgcc gtaatccgcg tactggaaga
    38461 tcggcgcatt tttatcttta ttgatggcga aaatggtttg cgatgcatta gcaccaacca
    38521 tgtgctggat ctgcccggag atccccaccg ccaggtacag ttcaggtttc agcatcaggt
    38581 tggagatacc gacatagcgt tcgtgctcca tccatttttc gttttccgcc accggacgag
    38641 aacaggccaa ctccgcacct atcgccttgc aaagctgttc tgccagcgca atgttctctt
    38701 tgctgccaat accgcgaccg acgctgacca ccagacgggc tttgtcgaga tcgacgctgt
    38761 tgctctggcg cgcctgggtt gccgtgcggg taatcgccac agccggagcc tgccactcca
    38821 cggtgtgcgt ttcgccagtg cgtgacgcgt ctggctgagc cgcatcgaac gtgccgctgc
    38881 tgatggtcag taccgcatac ggcgtggcaa tgcgttcttc gccaatcgcc agaccaccgt
    38941 aaaccatgtg tttcactgtc gctttaccgt cctgtacgct gacggtgctg gcatcgttag
    39001 acaccgccgc tttaaggcga taacccagtt ttgccgccag taatttgccg cgacgggtgt
    39061 ttggcagcag caccaggccg tctgcgccgt gctggcgaat agtgtcagcc atgacaccgg
    39121 cgtaatcttc gatcatccga tcgtccggtt tgccgtttaa tttccagaca tgattagcgc
    39181 cgagctggat tgcctgtacg ccgtcggcat cattgaggac aaaggtgttg atttgattag
    39241 ctaaagcctg cgcaccgttc atcagttccg gcagacgaga aggggtatcg ctgaataccc
    39301 agacttgaga aaacgtgttc atagcatccc ctgtaattaa atgactttgc gaagattttc
    39361 agcaaatgcg gcgatctgtt cttcgccgtc gccttcaatc acgatgcgct gacgttcgcg
    39421 ctgtttcggc gcggcaacct gttgttctga ccaggctgcc tctgcgttaa aaccaatatc
    39481 cgccgccgac catacctgga cgggcttttt cgccgcgccg agaatggctt tcatcgaagg
    39541 aatttgtggg gagttgatat cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa
    39601 ggtttcggtt tcatcttcca gttcgcgctc aacggtgagg gtatctgcag tcagggagat
    39661 aattttgctg acgccgttaa ctgccggaat attgaggatt tcgcccacca gcagaccaac
    39721 ctgctgggca taaaggtcgg aagaaccatc gccacagagg atcaaatcaa agcctgcttt
    39781 ctgggcggct gcagccagtg cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc
    39841 atcaatcacc acaatcagtt catccgggcc gcgcgatagc acatctttac gccctttggc
    39901 gttggtcagg gctttaccgc ccacacttaa ggccgtcacc tgcgcctctg ctgcctgttg
    39961 ctttagctgg caagccgctt caatagcgtt cagatcgtat tggcttattt tggcatcggc
    40021 tttgctgaag tctaatgaac catcagcatt attgaccgca atatcctgtt catcaggcac
    40081 gcacttatag caagtaataa tcttcattgc atctccagaa atcatgaagg aaacgtcgtt
    40141 catatcggtg gtgttaatta cagatatcac gccctttaat ttggaaataa aactttaatc
    40201 accaatattg aaaatgtcac acgcagataa atccaacttt caatattgtt aagttcctca
    40261 ccaatattga aaacatggcg tagcaaaaag aaattttcaa tattgcttt
//
LOCUS       DAZFYC010000034        39457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFYC010000034 DAZFYC010000000
VERSION     DAZFYC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.034"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..64)
                     /locus_tag="ACHKW5_001964"
     CDS             complement(<1..64)
                     /locus_tag="ACHKW5_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511371.1"
                     /translation="MIKNLPQIVLLNIVGLALFLS"
     gene            complement(103..1089)
                     /gene="yeiR"
                     /locus_tag="ACHKW5_001965"
     CDS             complement(103..1089)
                     /gene="yeiR"
                     /locus_tag="ACHKW5_001965"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO7511372.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DDALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1207..2673)
                     /gene="yeiQ"
                     /locus_tag="ACHKW5_001966"
                     /pseudo
     CDS             complement(1207..2673)
                     /gene="yeiQ"
                     /locus_tag="ACHKW5_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(2896..3468)
                     /gene="yeiP"
                     /locus_tag="ACHKW5_001967"
     CDS             complement(2896..3468)
                     /gene="yeiP"
                     /locus_tag="ACHKW5_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO7511373.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            3623..3877
                     /gene="yeiW"
                     /locus_tag="ACHKW5_001968"
     CDS             3623..3877
                     /gene="yeiW"
                     /locus_tag="ACHKW5_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7511374.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(3874..5052)
                     /gene="setB"
                     /locus_tag="ACHKW5_001969"
     CDS             complement(3874..5052)
                     /gene="setB"
                     /locus_tag="ACHKW5_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO7511375.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMGTAAGLEIPTMLIAGYFAK
                     RLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLMP
                     GQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKDV
                     "
     gene            5420..6550
                     /gene="fruB"
                     /locus_tag="ACHKW5_001970"
     CDS             5420..6550
                     /gene="fruB"
                     /locus_tag="ACHKW5_001970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO7511376.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSAPTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            6550..7488
                     /gene="fruK"
                     /locus_tag="ACHKW5_001971"
     CDS             6550..7488
                     /gene="fruK"
                     /locus_tag="ACHKW5_001971"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO7511377.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            7505..9196
                     /gene="fruA"
                     /locus_tag="ACHKW5_001972"
     CDS             7505..9196
                     /gene="fruA"
                     /locus_tag="ACHKW5_001972"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO7511378.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            9620..10561
                     /gene="psuK"
                     /locus_tag="ACHKW5_001973"
     CDS             9620..10561
                     /gene="psuK"
                     /locus_tag="ACHKW5_001973"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO7511379.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            10549..11487
                     /gene="psuG"
                     /locus_tag="ACHKW5_001974"
     CDS             10549..11487
                     /gene="psuG"
                     /locus_tag="ACHKW5_001974"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO7511380.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEEAINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            11581..12831
                     /locus_tag="ACHKW5_001975"
     CDS             11581..12831
                     /locus_tag="ACHKW5_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7511381.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12902..13600)
                     /gene="yeiL"
                     /locus_tag="ACHKW5_001976"
     CDS             complement(12902..13600)
                     /gene="yeiL"
                     /locus_tag="ACHKW5_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO7511382.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYQHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            13730..14671
                     /gene="rihB"
                     /locus_tag="ACHKW5_001977"
     CDS             13730..14671
                     /gene="rihB"
                     /locus_tag="ACHKW5_001977"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO7511383.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            14771..16021
                     /gene="nupX"
                     /locus_tag="ACHKW5_001978"
     CDS             14771..16021
                     /gene="nupX"
                     /locus_tag="ACHKW5_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7511384.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16077..17165)
                     /locus_tag="ACHKW5_001979"
     CDS             complement(16077..17165)
                     /locus_tag="ACHKW5_001979"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7511385.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17169..18026)
                     /gene="nfo"
                     /locus_tag="ACHKW5_001980"
     CDS             complement(17169..18026)
                     /gene="nfo"
                     /locus_tag="ACHKW5_001980"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO7511386.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18100..19149)
                     /gene="yeiH"
                     /locus_tag="ACHKW5_001981"
     CDS             complement(18100..19149)
                     /gene="yeiH"
                     /locus_tag="ACHKW5_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO7511387.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19248..20129
                     /gene="yieE"
                     /locus_tag="ACHKW5_001982"
     CDS             19248..20129
                     /gene="yieE"
                     /locus_tag="ACHKW5_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO7511388.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20334..21803
                     /gene="lysP"
                     /locus_tag="ACHKW5_001983"
     CDS             20334..21803
                     /gene="lysP"
                     /locus_tag="ACHKW5_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO7511389.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22096..24087
                     /gene="cirA"
                     /locus_tag="ACHKW5_001984"
     CDS             22096..24087
                     /gene="cirA"
                     /locus_tag="ACHKW5_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO7511390.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24119..24955)
                     /gene="yeiG"
                     /locus_tag="ACHKW5_001985"
     CDS             complement(24119..24955)
                     /gene="yeiG"
                     /locus_tag="ACHKW5_001985"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7511391.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25213..25881
                     /gene="folE"
                     /locus_tag="ACHKW5_001986"
     CDS             25213..25881
                     /gene="folE"
                     /locus_tag="ACHKW5_001986"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO7511392.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            25898..27055
                     /gene="yeiB"
                     /locus_tag="ACHKW5_001987"
     CDS             25898..27055
                     /gene="yeiB"
                     /locus_tag="ACHKW5_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO7511393.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            27197..28237
                     /gene="galS"
                     /locus_tag="ACHKW5_001988"
     CDS             27197..28237
                     /gene="galS"
                     /locus_tag="ACHKW5_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO7511394.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDSRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            28517..29515
                     /gene="mglB"
                     /locus_tag="ACHKW5_001989"
     CDS             28517..29515
                     /gene="mglB"
                     /locus_tag="ACHKW5_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO7511395.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            29576..31096
                     /gene="mglA"
                     /locus_tag="ACHKW5_001990"
     CDS             29576..31096
                     /gene="mglA"
                     /locus_tag="ACHKW5_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO7511396.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31112..32122
                     /gene="mglC"
                     /locus_tag="ACHKW5_001991"
     CDS             31112..32122
                     /gene="mglC"
                     /locus_tag="ACHKW5_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO7511397.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32193..33428)
                     /gene="preA"
                     /locus_tag="ACHKW5_001992"
     CDS             complement(32193..33428)
                     /gene="preA"
                     /locus_tag="ACHKW5_001992"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO7511398.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(33422..34660)
                     /gene="preT"
                     /locus_tag="ACHKW5_001993"
     CDS             complement(33422..34660)
                     /gene="preT"
                     /locus_tag="ACHKW5_001993"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO7511399.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLLLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(34979..35218)
                     /gene="yeiS"
                     /locus_tag="ACHKW5_001994"
     CDS             complement(34979..35218)
                     /gene="yeiS"
                     /locus_tag="ACHKW5_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO7511400.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35221..35940)
                     /gene="sanA"
                     /locus_tag="ACHKW5_001995"
     CDS             complement(35221..35940)
                     /gene="sanA"
                     /locus_tag="ACHKW5_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO7511401.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36090..36974)
                     /gene="cdd"
                     /locus_tag="ACHKW5_001996"
     CDS             complement(36090..36974)
                     /gene="cdd"
                     /locus_tag="ACHKW5_001996"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO7511402.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEEALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37104..37799)
                     /gene="yohK"
                     /locus_tag="ACHKW5_001997"
     CDS             complement(37104..37799)
                     /gene="yohK"
                     /locus_tag="ACHKW5_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO7511403.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(37796..38194)
                     /gene="yohJ"
                     /locus_tag="ACHKW5_001998"
     CDS             complement(37796..38194)
                     /gene="yohJ"
                     /locus_tag="ACHKW5_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO7511404.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            38433..39383
                     /gene="dusC"
                     /locus_tag="ACHKW5_001999"
     CDS             38433..39383
                     /gene="dusC"
                     /locus_tag="ACHKW5_001999"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO7511405.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         9673 a         9680 c        10354 g         9750 t
ORIGIN      
        1 aggaaagaaa cagcgcgagg ccgacaatat tcaacaacac tatttgcggc aaatttttaa
       61 tcatattttc tctaattatc ttactgaaaa acgcaggcaa ccttacgcgg tagtcgctaa
      121 acgaagcttc aacagcgcgc tctgcaaagc attccagtca gcttcgctgc tggaaatcag
      181 ctcaatacgg ctgtccggtg gcgcaacgtt ttgcgtttca atgtgcaggt catcgccctg
      241 acggttgatt cgtaccagcc cttcgggaat acgcagcacg cctttgacgc gttccaccgg
      301 tgcaagtcgc gcccattcca gaatgccaat ggtgtcgaat accgtatcag cgtcgaatat
      361 ccagccgcag gcctgatatc cttgcccgct gttcagactg cgacgccagc gttgatgctc
      421 tggcaggctt aacgctgcta accctttttt cacgacatgc tgatgagaat gcgcggcgct
      481 ggcgggcaac tcggctaaat tgcgacgcgg caaatccaga agatgaccgt caactttccc
      541 atgctcactg tgaattaatt gtcgatcgcc accattttgc tgccaccaac gctgtagcgc
      601 ttgctcactt tcgggcgtcg ctcggtcgga tttattggcg acaatgatgt ctgcggcagc
      661 cagctggtca cggaagtttt cattgctggc gcttttttcg tccagcagca agcgcggatc
      721 gagaatgcac aaggtggcgc gcagatctat ccacggttca tagactggtg cggttaacag
      781 atcgaggatc tgtttcggat ggcccagccc ggtcggctct atcaacaagc ggtctggttt
      841 tccctgacgc agtaaggtat tcaaccctac ctgcatgggt aaaccattaa cgcagcacat
      901 gcagccgccg gggatctctt tcagcaatgc gccgctatcg gcgagcaaag catcatcaat
      961 tccgacttcc ccaaattcat taaccagtac cgcccatttt tcgttgggat ctttatgggc
     1021 taacagatga agaatcgacg tggttttccc gctgccgaga aaaccggtga tgaggttggt
     1081 cctggtcaca acggctccgc aatactctgg taagttaact gagcgttaat cctggctaaa
     1141 aaaggcagaa aagagaaggg gagagcggac gaaggtgagc ttcatccgct ttaaccgcag
     1201 aaatcgttaa attttgaggg tgtttaatag cgcctgatgt gcgccgaatt gtacgatttg
     1261 ttgccaggct tgttcgatgg cctgcacaaa atgcgggtta tctggcagat catcaccgaa
     1321 aacttcacgc agggaaagca gggcggttac gcgttgttca ctgctgctgc ccgcaacaag
     1381 ttcgcgaatt ttatcgctaa gcggatcgcg aacatcaatg gcatttccgg catcatcaac
     1441 gccgctgacg taacgcatcc agccagcaac gcctaatgcc agcaacgacc agtccgtttc
     1501 gcaccccaga tgtatgcgaa tacctgccag catacgttgc ggtaatttct ggctaccatc
     1561 cattgcgatt tgccaggtct tatgtttcag cgccggatta gcaaaacgtg caattaactt
     1621 gtccgcatat tgtgttaaat cgacatcttt aatttgtagt gtcggcgctt gctcattcag
     1681 cattaatgtt ctggcggcat ggcgaaatgc gcgatcctgc atgcaatcac tgatatgggc
     1741 gaatcctgaa aggtaaccca gataagcgag aaaagagtgg ctgccattaa gcatacgcag
     1801 tttcatctct tcccatggca ggacatcatt caccatttgt acacctgcga cttcccaggc
     1861 aggacgccca gcgacgaagt tatcttccac cacccactgg ataaacggtt cgcagctaat
     1921 cgcgcaggga tcatttaccc ccagatgctg gctgatttct gccagtgatt cgtcggttgc
     1981 agccggaaca atgcggtcga ccatggttcc cggaaaactg acgtgttatt ttatccaccc
     2041 ggcgagttct ggcgaacgtt tttctgccat tcccagcacc gcgtttttca ccacatggcc
     2101 attgtcggga atattgtcgc aggagagcac ggtaaacggt gtaaggcctc gctcacggcg
     2161 gcgtttcagt gcttcgacga gaatacccgg tgcggagtga ggttcttcag gggtttgtag
     2221 atcgtgaata atccgcggat tactggtgtc gagtgcaccg gtggccgggt caatacaata
     2281 gcctttttcg gtaatcgtca gggaaacaat tgccacctgt ggctcgcaaa atttctcaat
     2341 aattgccgct aaggaatcca gttttgcatt aaggcattcg tgaacggcac cgacaattat
     2401 cacctgattg ccgtccgcac ctttttccag cacggtatat aaatggttct gtgcgcggag
     2461 ctggctcatc agttgatcac cgctgaacaa gctgatttca cagatccccc agtcgccgcc
     2521 ctgggcattc agcacacgat cggtcagtaa cgcctgatga gcgcggtgaa aggcgccaaa
     2581 gccaaaatga acgatacgtg attgcaactg ctggcgatcg tagcgtggag cgtgtatatg
     2641 atgcgggagc gtaacggagg caattgtctt cataggagtc acgtcttagt aacgataaat
     2701 taacaatgac agtgtaaagt tatatgacag caattgaaat tggtctggat tgttaatgcg
     2761 atgaagtgtg agggcgatca atcaacgagt gaaaattgag ggcgtaaggt gttcaggaag
     2821 cgcaaaatag aagattggta taataaatta aaaaagggac agcctgagct atcccttttc
     2881 tgcatgcggc tgaagttagt cagcacgccc catataacgg cgttcttcga tatggatacg
     2941 aattttttcg cccgggctta agtattctgg tacctgaatc accagaccag tgctcaatgt
     3001 cgccggtttg ttacgggcgc tggcagatgc ccctttgatg cctggtgcgg tttcaacgat
     3061 ttccagatca acggtctgcg gcagctcaag cgccagcagt tgaccgtccc aggtcagcac
     3121 ctgcatgtcc ggcatgccgc cctctggcat aaacagcaac tcttcttcaa tctgatcttt
     3181 ggtgaaggta tacggggtat agtcttcttt atccataaag acatattcgt tgccatcgac
     3241 ataggagaag tcaacgtaac ggcgggtcag cgtcacggtg tcaacgatat catcaccttt
     3301 gaaacgctct tctactttca gcccggtacg gacatcagaa aaacgcattt tgtacagcgt
     3361 agcggcaccg cgggcagtgg gcgactgaat atcaatatcc ttaaccagca gcagtttgcc
     3421 gttgtaattc agtaccatac cttttttaat ttcgttcgct cttggcatcg aaaaattcct
     3481 gtagtcgaaa atgtcaaaaa tatcgcgaca aagtactcgc gacgggcctt tcgggcaagt
     3541 ggtattcgca cttttgctgg tgcaaaaaag gtggtactgt gcgcgctcat caatccggtg
     3601 gttaacctta agagaacaac gtatgaaatg ccgtccgggt tgcggggcgt gttgcactgc
     3661 cccttcaatt tccagcccta ttcccggtat gccagatggc aagcccgcca atacgccctg
     3721 cattcagctt gatgaacagc aacgctgtaa aatattcacc tcgccactgc gaccgaaagt
     3781 ctgtgcaggc ttacaagcca gcgctgaaat gtgcggtaac tcgcgtcagc aggccatgac
     3841 ctggcttatc gatctggaaa tgctgaccgc gccttaaaca tctttaatcc gcagtaagca
     3901 aaacagagtg gcgataatca tcaccatcgc aaaccagaac acagcgtgat aattccagat
     3961 ctcggcgacg atgcccgcca ctgatcctgc gatgatccag cccacgcgcg acgtgttggt
     4021 atagagcgtg gtggctgaac ccgcctgacc gggcatcaga tcctgaaaat agagcatccc
     4081 gatgccgccc agaatgccaa taaaaatagc atttagcagc tgcaagccca acagaatgac
     4141 aggtgaatgc gccatcagca ttcctgcgta aaaacagacg ccacccacgg cagcaacgcg
     4201 cattaagaaa cgcttaccca gacgtttggc gaaatatccg gcaatcaaca tcgtcgggat
     4261 ttccagcccg gcggcggtcc ccatcacacc ggccagtttc tcgggaagat gcagttcgtt
     4321 gataataaat agcggcatgt tgatgatgta caggctgttc gagccccaca tcaatgtaca
     4381 aatgacaaac agcagcagcg tatcgcgacg gttacggcgc ggcgcttcga tcgtgccggt
     4441 cgccagcgga agctcttttt gcatcgacgg taaaaacagc cacaccatca caccgcaaac
     4501 aataaacgct accgctgcgc tcagatacat taccgtaaag ctgaaaccca tcgctaaggc
     4561 ataagccagc ggtgggccaa tgacccatgc cagtgaaacc tgagcgcgta aaaaagagct
     4621 gaacatcacc gcctcacgtc cggttttgtc ggcatgttca cgggcaaggg caaacatttg
     4681 cgggttagcg gtcgagccaa agctgctaag aaagacgcca acgaatagca aaacaaagta
     4741 gttgcgattc caggcaaaaa gggtgcaggc cagcacgcct aacaggcagc aaaagacaat
     4801 cagcgatttg cgatcgccgc gcttatcaga gcgcccggcg agaaactgac ttaccagaat
     4861 cccaatgaca gcgctgccgg taaagaagaa tcccaccatc gccggacgag catgtacttc
     4921 atcagtaaga aaaatactga gtgtcggggt ttgcagagcg cccgcaatac cggtgagaaa
     4981 ggcaacgatt aaaaacgccg tcgaggtcag gtcaaacgat ttcgcgctgg agactgcggg
     5041 ggagttatgc atgctggtcg atcactggaa gaggaagacg cggagtttac gctgcttgtc
     5101 agaaaataaa cagacagcga aatcaaaagc gccacgaata atcaaaatgc catttcactg
     5161 ttgctgattg gctgctgacg ctgctgaaat gaaattgctg ataacggatt ttcccatcag
     5221 caattaggaa aaatggcaaa aaattgtgca gcacatcaaa cttttgctca taactttacg
     5281 gctttccttg cgtgctgaaa cagaagtatt atgctttctt gaaacgtttc agcgcgatct
     5341 tgtctttaac cctaagccag gttggcgctt tttttctcat agaggctgaa tcgtttcaat
     5401 tcagcaagag aggagaacta tgttccagtt atccgtacag gacatccatc cgggcgaaaa
     5461 ggccggagac aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa
     5521 tgtagcagaa ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct caacgttcct
     5581 cggcaatggt attgctattc cacacggcac taccgacacc cgcgatcagg tgctgaaaac
     5641 cggcgttcag gtatttcagt tcccggaagg cgtcacctgg ggtgacggtc aggtagcgta
     5701 cgtggcaatc ggtattgctg ccagctcgga tgaacatctg ggcctgctac gccagctgac
     5761 ccacgtactg agcgatgatt ccgttgctga acaactgaag tcagcaccaa cagcagaaga
     5821 acttcgcgca ttactgatgg gcgaaaagca gagtgagcag ctgaagctcg acaacgaaat
     5881 gctgacgctg gatatcgtcg ccagcgatct gctgactctt caggcgctga acgctgcgcg
     5941 tctgaaagag gcgagggcag ttgacgccac tttcgtcacc aaagccatca atgaacaacc
     6001 gctgaacctc ggacagggta tctggctgag cgatagcgcc gaaggcaatc tgcgtagcgc
     6061 gattgcggta agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg cagccatgct
     6121 ggtgagtgtg gcgatgaatg acgatcagcc cattgcggtt cttaagcgtc tcgctgattt
     6181 gttgctcgac aataaagctg accgcttgct gaaagcggat gcggcaacgt tgctggcgct
     6241 gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa
     6301 tgaacacggc ctgcatgctc gtccaggtac catgctggtc aataccatta aacaatttaa
     6361 cagtgatatt accgtgacaa accttgatgg caccggcaaa ccggcaaacg gacgtagtct
     6421 gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg
     6481 tgcagatgct gaacaggcgc tgaaagcaat cggcgacgct atcgctgctg gtcttgggga
     6541 gggcgcataa tgagcagacg tgttgctact atcaccctta atccggctta tgaccttgtt
     6601 ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat
     6661 gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag acctgggaat tgatgtcacc
     6721 gttggcggct tcctcggtaa agacaatcag gatggttttc agcaactgtt cagcgagctg
     6781 ggcattgcca accgtttcca ggttgtacag gggcgcaccc gaattaacgt taagctgacg
     6841 gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt ttgaagtcac ccccgccgac
     6901 tgggaacgct ttgtgactga ttctctgagc tggctcggtc agttcgatat ggtctgtgtc
     6961 agcggaagct taccgtcagg cgtcagcccg gaagcgttca ccgactggat gactcgcctg
     7021 cgtagtcagt gtccttgcat tatctttgat agtagccgtg aagcgttagt agcaggtttg
     7081 aaagcggcac cgtggctggt gaaacctaac cgccgcgagc tggaaatctg ggcaggccgt
     7141 aaactgcctg aaatgaaaga tgtgattgaa gctgcacatg cgctacgtga acaaggtatt
     7201 gcgcatgttg ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa
     7261 tggatcgcca aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg
     7321 gttggtggcc tgatttatgg cttgctgatg cgtgaatcca gtgaacacac actgcgtctg
     7381 gcgactgctg ttgcagccct ggcggtaagt caaagcaatg tgggtattac cgatcgtccg
     7441 cagttggccg caatgatggc gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg
     7501 cataatgaaa acgctgctga ttattgacgc taatctcggt caggcacgcg cctatatggc
     7561 gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa
     7621 cgacgcagaa atggcgattg ttctcggtga ttccatcccg aatgacagcg cgctgaacgg
     7681 taaaaatgtc tggctgggtg atatttcccg ggcagttgcg caccctgagc tgttcctgag
     7741 tgaagccaaa ggccatgcga aaccttacac tgcgccggtc actgcgacag caccggttgc
     7801 cgccagcggt ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg tagcacacac
     7861 ctttatggcg gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt
     7921 tgaaacccgt ggttctgttg gcgcgggtaa tgcaatcact cccgaggaag tagccgcagc
     7981 ggatctggtg attgtggcgg cagatatcga agtggatctg gcgaaatttg ctggtaaacc
     8041 gatgtatcgt acctctaccg gtctggcgct gaagaaaacc gcgcaggaac tggataaagc
     8101 ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct caaacggcga cctctgaagg
     8161 taagaaagag agtgcaggcg cataccgtca cttgctgacg ggcgtctctt acatgctgcc
     8221 gatggtcgtt gcaggtggtc tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt
     8281 taaagagccg ggtacgttgg cagcggcgct gatgcagatt ggtggtggtt cagcctttgc
     8341 gctgatggtg ccggtactgg caggttacat tgccttctcc attgccgatc gtccgggtct
     8401 cacgccgggt ctgattggcg gtatgctggc ggtcagcacc ggttctggct tcattggcgg
     8461 tattattgcg ggcttcctgg ctggttacat tgcgaagtta atcagtacgc aactgaaact
     8521 gccacagagt atggaggcgc tgaaaccgat cctgatcatt ccgctaattt ccagtctggt
     8581 ggtcggtctg gcgatgatct acctgatcgg taaaccggtt gctggcattc tcgaagggtt
     8641 gactcactgg ctgcagacca tggggactgc gaatgcggtt ctgctggggg cgatcctcgg
     8701 tggcatgatg tgtactgaca tgggcggtcc ggtaaataaa gcagcgtacg cattcggtgt
     8761 gggtctgctg agtactcaaa cctatggccc gatggcggcg attatggcgg caggtatggt
     8821 gccaccgctg gcaatgggcc tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca
     8881 gcaggaaggt ggcaaagccg ctctggtact gggactgtgc tttatttcgg aaggtgcaat
     8941 tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgc tgtatcgtgg gtggggcgct
     9001 gactggcgca atctcaatgg cgattggtgc gaaactgatg gcaccgcacg gtggtctgtt
     9061 tgttctgctg atccctggcg ctattacgcc ggtattgggt tacctggtag caattattgc
     9121 cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa cgtccggaag tggacgcagt
     9181 agcgaaagcc gcgtaataaa aggtgtgtta cagggcagaa atttactgcc ctgtatcatt
     9241 ggatgagcgc aaagagaatg accggtttgt gaatgtaatt ttatgaattt aaatggtttt
     9301 atctttcagt agaaacctga aacgttcatc aaacactaag gttatttcct caagggcaga
     9361 ttcttttctg ccctttttta gtgttcctgt tctgtgcatc ccgtcacaaa ttcctcgctt
     9421 ttgtaattct ggcatttaat cgcattttgt gagcctgctc aaatctgccc gctttgcctc
     9481 tctggcggca atttacttta cgtaaaacat gcatactatg agcacatgtt taaatatgaa
     9541 cacatgttta aactgtcttt gcagtatcta tattcttgtg gattgacagg gttggtaatt
     9601 ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa
     9661 tattgatgtc gccggatatt cacatgaatc attaaattat gcggattcaa atccaggtaa
     9721 aataaaattt acacctggtg gagtagggcg aaatattgca caaaacctgg cgttgctggg
     9781 taacaaagcc tggttactga gcgcagtagg tagtgatttt tatggtcaat cgctgctaac
     9841 gcaaaccaat caatctggcg tttatgtcga taaatgcctg attgtgccgg gagaaaatac
     9901 gtcgagttat ttatcattac tcgataatac cggtgaaatg ctggttgcta taaatgacat
     9961 gaatattagc aacgctatta cagctgaata tctcgcacag caccgtgaat ttattcagag
    10021 ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag gcactggcat ggattctgga
    10081 taatgctgcc aacgtacccg tatttgtcga tccggtttca gcgtggaaat gtgtcaaagt
    10141 gcgcgaccgt ctaaatcaga tccacactct gaagccaaac cgccttgaag cggaaacact
    10201 gagtgggatt gcgctgtcag ggcgtggaga tgtggcaaaa gttgctgcct ggttccatca
    10261 acatggcctg aaccgactgg tattgagcat gggcggcgat ggcgtttatt acagtgatat
    10321 cagcggtgaa agtggctggt ctgccccgat caaaaccaat gttattaatg ttaccggagc
    10381 gggcgatgcc atgatggcgg gacttgcttc gtgttgggta gacggaatgc cgtttgccga
    10441 atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat acaccaataa
    10501 ccccgattta tcgattgcca acgttatatc gttagtggag aacgcagaat gtctgaatta
    10561 aaaatttccc ctgaattatt acaaatttcc ccggaagtgc aggacgcttt aaaaaacaaa
    10621 aaaccggttg ttgcgctgga atcgaccata atttctcacg gaatgccatt cccacaaaat
    10681 gcccaaaccg caattgaagt agaagaaact attcgtaaac agggcgcagt accagccact
    10741 atcgccatta ttggcggtgt gatgaaagtg ggcttaagta aagaagaaat cgaattgctg
    10801 ggccgtgaag gacataacgt gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc
    10861 gccggaaaaa atggcgcaac cactgtggct tcaacgatga ttattgcggc gcttgccgga
    10921 attaaagtat ttgccaccgg gggaattggt ggtgtgcatc gcggggcgga acataccttc
    10981 gatatttctg ccgatttaca ggaactggca aataccaatg tcactgttgt ttgtgcgggg
    11041 gcaaaatcta ttctcgattt aggattaaca acagaatatt tagaaacctt cggtgtaccg
    11101 ttaattggtt atcagacaaa agcgctgcct gcgtttttct gccgaaccag cccatttgaa
    11161 gtcagcattc gtcttgacag tgcccgcgaa attgctcgcg cgatggcagt gaagtggcaa
    11221 accggtttga acggcggcct ggtagtggcg aatccgatcc cggaacagtt tgcgatgcca
    11281 gaagaagcca tcaatgcagc aattgatcaa gccgttgctg aagccgaaga acagggtgtt
    11341 attgggaaag agagtacacc gttcctgctg gcgcgtgttg cggagctaac aggcggtgat
    11401 agcctgaaat ccaatattca actggtcttt aataacgcca ttctggcgag cgaaattgcc
    11461 aaagaatatc agcgtctcgt gggttaattt ctggcgggca gaaagccgtc gcccgtactc
    11521 atcaaacata ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg gaaaatcatt
    11581 atggatataa tgagaagtgt tgtggggatg gtggtattgc tggcaattgc ctttgtgttg
    11641 tcggtaaata aaaagagcat cagcttgcgc acggttggcg ctgcgctgct gctgcaaatt
    11701 gccattggtg gcatcatgct ctattttccc ccgggaaaat gggcagtaga gcaggcggca
    11761 ttaggcgtcc ataaagtgat gtcctacagt gacgcaggaa gtgcctttat ttttggttcg
    11821 ctggtcggtc ccaaaatgga tgttctgttt gacggtgcgg gctttatctt cgcctttcgc
    11881 gtactcccgg cgattatttt cgttaccgcg cttatcagct tgctgtacta cattggcgta
    11941 atggggctgc tgattcgtat tcttggcagt atttttcaga aagccctcaa catcagcaaa
    12001 atcgaatctt ttgtcgcagt caccactatt ttcctcgggc aaaatgagat cccggcgatc
    12061 gttaaacctt ttattgatcg catgaatcgc aacgagctat ttacggctat ttgtagcggc
    12121 atggcgtcta ttgcgggttc gatgatgatt ggctatgccg gaatgggcgt gccgattgac
    12181 tatttgttag ccgcttcgct gatggctatt cctggcggta tcctgtttgc acgtattctt
    12241 agtccggcga cggaaccttc gcaagttacg tttgaaaatc tgtcgtttag cgaaacaccg
    12301 ccaaaaagca ttattgaagc tgcggcgaac ggtgcaatga ccgggctgaa aatcgccgcc
    12361 ggtgtggcga cggtggtaat ggcatttgtg gcaattattg cgctgatcaa cggcattatt
    12421 ggtggagttg gcggctggtt tggttttgcc aatgtctctc tggaaagcat tttcggctat
    12481 gtgctggccc cgttggcatg gattatgggg gtggactgga gtgatgcaaa tcttgccgga
    12541 agtttgattg ggcagaaact ggcgattaat gagtttgttg cttatctcaa tttctcgccc
    12601 tatctgcaaa cgagcggcac gctggatgtc aagaccattg ccattatctc gttcgccctc
    12661 tgtgggtttg caaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg
    12721 ccaaaacgtg ctccagaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt
    12781 tccaacctga tgagtgcgac tattgccggg ttctttattg gtctggcgta atgctcgatt
    12841 tgtagaccgg ataaaacgta ttaagcgttg catccggcaa tcgcgctatt gcaaataatt
    12901 tttactgcat catcccggag aatttattct ccgggtccat ctccagcgcc agtcctgaca
    12961 actgctttct gtttttaatg agataccctt tcttgctttt agttaataaa ccgtcgtgaa
    13021 tgaattgcgc gagaacatat aaaagatgtt gataagaaac gcctaaatac tctgccgctt
    13081 gcgtatgctt ttcgtgataa agatcacctt cctgcgagag taatataaat gctgccaggc
    13141 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
    13201 gacttaaggt gacgcagagt tttcgtaaaa atagcgtgtc gtttaataac cgcgggcggt
    13261 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
    13321 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggggcggca aagaaatcga
    13381 tcagcgacac gcgaccatta gctagcgtgg cgtaaagcct ggcgcggcct cgcgtcaggt
    13441 aaaacagcca ggacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
    13501 acagccgcgt atcggctgaa acatccgtta aaaagcaatc cttaaacgcg gattcactca
    13561 tcaattgctg cttaagatca ttattgtgga tttctttcac gatgtttatc cgctatgaga
    13621 tttctcatat ttagcatacg tacgcggttg gtaagattca atcatcggcc cggtcgcatt
    13681 ttctttgctg tagaaaaaat aacgcaactg gaaacagagg aaataaacaa tggaaaagag
    13741 aaaaattatt ctggattgtg atccgggtca tgatgatgct attgctataa tgatggcggc
    13801 gaaacatccg gcaatagatt tattaggcat cactattgta gcgggtaatc agacgcttga
    13861 taaaacatta attaatggcc tgaatgtttg ccagaaactg gagattaatg ttccggttta
    13921 tgcggggatg ccgcaaccca ttatgcgtca acaaatcgtt gccgataata ttcacggtga
    13981 aaccggactg gatggcccgg tattcgagcc gctgacccgc caggcagaaa gtacccacgc
    14041 ggtgaaatac atcattgata ccctgatggc aagcgatggc gatatcactc tggtgccggt
    14101 tggtccgctt tcaaatatcg cggtggcaat gcgtatgcaa cccgcgatcc tgcccaaaat
    14161 ccgtgaaatt gtgctaatgg gcggcgctta cggtacaggc aacttcacgc catctgccga
    14221 gttcaacatc tttgctgacc cggaagcagc ccgcgtggtg ttcacctctg gcgttccatt
    14281 agtgatgatg ggcctcgatc tcaccaacca gaccgtttgc accccggacg tgattgctcg
    14341 gatggaaagg gcaggcggcc ctgccggaga gctgttcagc gacatcatga acttcactct
    14401 caaaacgcag ttcgaaaact acggcctggc tggcggcccg gtgcacgacg ccacctgcat
    14461 cggttatctg attaaccctg atggcattaa aacccaggag atgtacgtcg aagtggacgt
    14521 caacagtggc ccttgctatg ggcgcaccgt ctgcgacgag ctgggcgttc ttggcaagcc
    14581 cgccaatacc aaagtcggca tcactattga taccgactgg ttctggggct tagtcgaaga
    14641 gtgcgtgcgc ggctacatca aaacccatta agcgttgata acaagccggg ccgatagcgc
    14701 aagatgcagg cccggctatt tctgatgggc agaaagccgt cgcccaatat gacatgaaga
    14761 gataatcact atggatgtca tgagaagtgt tctgggaatg gtggtattgc tggcaattgc
    14821 atttttgctg tcagtaaaca agaagaagat cagcctgcgt accgttggcg cggcgttagt
    14881 gttacaggtt gtgattggcg gcattatgct ttggttaccg ccagggcgtt gggtcgctga
    14941 aaaagtcgct tttggcgtgc ataaagtgat ggcgtacagc gacgcgggta gcgcatttat
    15001 cttcggttcg ctggtcgggc cgaaaatgga cacgctgttt gatggcgcag gatttatctt
    15061 tggtttcaga gtattaccgg caattatctt cgttacagca ctggtgagta ttctctacta
    15121 catcggtgtg atggggattt taattcgcat tctcggcggt atcttccaga aagcgttaaa
    15181 tatcagcaag atcgagtcat tcgtcgcggt cactaccatt ttcctcgggc aaaacgaaat
    15241 tccggcaatc gtcaaaccct ttatcgatcg tctgaatcgc aatgaattat ttacagcgat
    15301 ttgtagtggc atggcctcga ttgctggttc gacaatgatt ggttacgccg ccctgggcgt
    15361 acctgtggaa tatttgctgg cggcatcgtt aatggcgatc ccaggcggga tcttgtttgc
    15421 ccgcctgtta agcccggcta cggaatcttc gcaggtttcc tttaataacc tctctttcac
    15481 cgaaacaccg ccaaaaagca ttattgaagc cgctgcgaca ggggcaatga ccgggctgaa
    15541 aatcgccgcc ggtgtagcga cagttgttat ggcatttgtc gccatcattg cgttaattaa
    15601 tggtattatc ggcggcgttg gcggctggtt tggttttgca catgcctcgc tggagtccat
    15661 tttaggttac ctgttggccc cattggcgtg ggtgatgggg gttgactgga gtgatgcaaa
    15721 tcttgccggg agtttgattg ggcagaagct ggcgatcaat gaatttgtcg cttatctcaa
    15781 tttctcgcca tatctgcaaa cgggtggcac tctggatgct aaaaccgtgg cgattatttc
    15841 tttcgcgttg tgcggtttcg ctaactttgg ttctatcggg gtggtggtgg gggcgttttc
    15901 tgcggttgcg ccacaccgtg cgccggaaat cgcccaactt ggtttacgcg cgctggcggc
    15961 ggcgacactt tctaacctga tgagtgctac tattgcagga ttctttattg gtttagcgta
    16021 gtgcgcaatt tatgagccgg ataagatgcg gtcaagcgtc gaatccggca acattgttac
    16081 accattggca ctaatgaaag cgcgttatcg gcagacaggg tagggttgtt taagctgccg
    16141 ctggcgcgcg aaattgccgc gcaggctacc gcaaaacggg cgctgtcgcg gaaactgtat
    16201 ccttcaagaa agctatatac caagcccgcc ataaaaccat cgtcagcacc aaaactgtct
    16261 actgtcgtat gcgctggcgc ggtcagcaaa aattgttctc catccttatc gctgcaaaat
    16321 acagattcat ctttcagata aacaaaaatg cgtttaacac cttgttgatg cagcgaattt
    16381 acagctctga tgcgatcgtt gtcgtcttta atcggttgcc cccacaaaat ttccagttca
    16441 ttttgtgttg gtttcagagt gtggatccgg ctgtaccagt tttttacttt gttcgcttta
    16501 aattcggaaa cggtatcgac aaacatcgga atttcatcag caatggtaaa gacccactcc
    16561 agggcttcag gtgtgagatt acaatcagcc agcaccacac cagcatggcg aatcaagtcc
    16621 cgtgaagtat tgagcagttg aggcgttagc tgttgcagaa tatgtgtgtc attgattgcc
    16681 aggatagttt cttcctgctt attagcgata gcgagataag ttgcagtgct gtgtccatgc
    16741 agacgaatgc agtttgaaac gttcacgccc gcgcggcgcg tttcttccag taatgtctcg
    16801 ccgtaaaaat cgttgccaat agctgatatt aaatgcacat cccgaccaag cagagccagg
    16861 ttatgcgcga tattacgtcc gacgccgcct gcggaacagt gaacagaacc tggatgagag
    16921 gctgcttgcg ggtaacggat atccgccatt ccgcgaatat ccatattgat tgcacccaca
    16981 accacacagt aatcctgctc ggtaaggata tatcctttgc ctttaatcaa acccttgcgc
    17041 atcaaatcca taatatgagc ggcaacacgt gagcggctga tttgcagaat atcggcgatt
    17101 tcattctgtt gaatcagcgg gttacgccgt aaaattttaa gaatctgctt ttccctgtcg
    17161 ttcatcgatc aggccaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    17221 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgtcc
    17281 tgcatgatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    17341 tcaacgcggc tgccaaaggt gcttttcgca tcgttaaggt gcatcccgcg cagatactta
    17401 aagccaacaa tacgggcaaa atccgcgaac gttttctcgc attcggctgg agtacgcaaa
    17461 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacacg ggatttatct
    17521 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    17581 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    17641 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    17701 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    17761 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttaatcag atagctgtcg
    17821 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggccgcttt gaattcatcg
    17881 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    17941 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    18001 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    18061 ggtaatgata gcgggttaac aggagcaaga tgtagtggtt tatgcgatga cgctttgaat
    18121 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    18181 cagaggtttc gccccggctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    18241 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    18301 taacaggtgg aacgagttaa agatggcaac gacgatgaac aatatggcaa accacggaat
    18361 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    18421 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    18481 ttccgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    18541 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    18601 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    18661 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    18721 gatactgcta ccggcaccga tcaaccagct ggtgtgctta tccagaccaa acactttctg
    18781 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    18841 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    18901 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    18961 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    19021 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    19081 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    19141 attcgtcata accctctcct ttaaccgggc ataaggttac ggctaactgg tttaaaaata
    19201 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    19261 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    19321 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gaccgacctg gaagggcagc
    19381 ttggcgtgca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    19441 tgctctatcc gcgtgcgttg gcattgcttg aacaggcggt tgaaatcgaa caactgtttc
    19501 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    19561 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    19621 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggttttattg
    19681 aaggaccgtg ccacagcact gaaattattt ctgaaccgtg gctggaagac gagctggtgg
    19741 ttttcgccgc gccgacttcg ccgttggccc gtggtccggt caccttagag cagctggccg
    19801 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggagattgtc gattatctat
    19861 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    19921 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgggtg attgaagatc
    19981 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    20041 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    20101 attgcgatcc cgcgaatgta ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    20161 cgatttcgtg acgcagcgcc ctcagcatgc attcgccaga aaagagattg gctgctttac
    20221 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    20281 gccaatcgtc tgctacaatc gcgcctcatt tttaagatgg atagcatttt tgtatggttt
    20341 ccgaaactaa aaccacagaa gcgccgggct tacgccgtga attaaaggcg cgtcacctga
    20401 cgatgattgc cattggcggt tccatcggta caggtctttt tgttgcctct ggcgcaacga
    20461 tttctcaggc aggtccgggc ggggcattgc tctcgtatat gctgattggc ctgatggttt
    20521 acttcctgat gaccagtctc ggtgaactgg ctgcatatat gccggtttcc ggttcgtttg
    20581 ccacttacgg tcagaactat gttgaagaag gctttggctt cgcgctgggc tggaactact
    20641 ggtacaactg ggcggtgact atcgccgttg acctggttgc agctcagctg gtcatgagct
    20701 ggtggttccc ggatacaccg ggctggatct ggagtgcgtt gttcctcggc gttatcttcc
    20761 tgctgaacta catctcagtt cgtggctttg gtgaagcgga atactggttc tcactgatca
    20821 aagtcacgac agttattgtc tttatcatcg ttggcgtgct gatgattatc ggtatcttca
    20881 aaggcgcgca gcctgcgggc tggagcaact ggacaatcgg cgaagcgccg tttgctggtg
    20941 gttttgcggc gatgatcggc gtagctatga ttgtcggctt ctctttccag ggaaccgagc
    21001 tgatcggtat tgctgcaggc gagtccgaag atccggcgaa aaacattcca cgcgcggtac
    21061 gtcaggtgtt ctggcgaatc ctgttgttct atgtgttcgc gatcctgatt atcagcctga
    21121 ttattccgta caccgatccg agcctgctgc gtaacgatgt taaagacatc agcgttagtc
    21181 cgttcaccct ggtgttccag cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag
    21241 ttattctgac ggcggtgctg tcagcgggta actccggtat gtatgcgtct actcgtatgc
    21301 tgtacaccct ggcgtgtgac ggtaaagcgc cgcgcatttt cgctaaactg tcgcgtggtg
    21361 gcgtgccgcg taatgcgctg tatgcgacga cggtgattgc cggtctgtgc ttcctgacct
    21421 ccatgtttgg caaccagacg gtatacctgt ggctgctgaa cacctccggg atgacgggtt
    21481 ttatcgcctg gctggggatt gccattagcc actatcgctt ccgtcgcggt tacgtattgc
    21541 agggacacga cattaacgat ctgccgtacc gttcaggttt cttcccactg gggccgatct
    21601 tcgcattcat tctgtgtctg attatcactt tgggccagaa ctacgaagcg ttcctgaaag
    21661 ataccattga ctggggcggc gtagcggcaa cgtatattgg tatcccgctg ttcctgatta
    21721 tttggttcgg ctacaagctg attaaaggaa ctcacttcgt acgttacagc gaaatgaagt
    21781 tcccgcagaa cgataagaaa taagtttcct cccttccttg ctaagccctc tcaaccgaga
    21841 gggctttttc aattccattt ccctgacaaa tcatgcggat ataaaattta acatttggat
    21901 tgataattgt tatcgtttgc attatcgtta cgccgcaatc aaaaaaggct gacaaatcag
    21961 aggctgttcc ggctttctgg gatgatcacc tgcatacaaa taagtccacc gcgatgctgc
    22021 cgtacgcaag gggacgtgaa gaagatgtga gcgataaccc attttatttt cgtagttacc
    22081 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    22141 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    22201 actgcatctt ccgttgaaca aaacctcaaa gatgcacccg ccagtatcag cgtcattacc
    22261 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    22321 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    22381 agcagctaca ccctgattct cgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    22441 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    22501 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    22561 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    22621 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    22681 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    22741 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    22801 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    22861 ggtttcgacc gtcaggatcg tgattccgac tcgctggaca aaaaccgcct ggaacgccag
    22921 aactactccg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatactac
    22981 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    23041 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    23101 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    23161 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    23221 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    23281 ccgcgtgcct acctggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    23341 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    23401 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    23461 gagctggggc tttactacat gggtgaagaa ggctggctgg aaggggttga atccagcgtt
    23521 accgttttcc gtaacgatgt gaaagatcgc atcagcatca gccgtacgtc tgacgtcaac
    23581 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    23641 ccggtattta gctactacaa cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    23701 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    23761 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    23821 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    23881 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    23941 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    24001 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    24061 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    24121 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    24181 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    24241 cggcttctgc cagtaccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    24301 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactgt
    24361 cccattccag ccatgcattt ttgtcctcac ctaaatagcg gctaaacgct ttgattcccc
    24421 acgggacgct gcacggattc acaattggcg caaaggccga aacgctggtg tatttacccg
    24481 gatttttcag cgccataatc agcgcaccgt gaccacccat tgagtgaccg ctaatggcgc
    24541 agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg cgcagataat
    24601 catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa aagcctgcgc
    24661 cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg
    24721 gcatcaccag tacaatcccc agttctgccg ccacgcgctg ggcacccgcc ttggtggtga
    24781 agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc ggggtgtgat
    24841 cacgaggggg tgggagaaag atactgaacg tcatcgggca gtttaaggta ctggagtcgt
    24901 gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg
    24961 gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg tgctgtgagt
    25021 aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa aggcagcggc
    25081 aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg cacaataatc
    25141 aggctgtaaa tcagcttaat aactttgccc ccacgcaggg cggaggcgtc acacctgcag
    25201 gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga agcgttagtt
    25261 gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc
    25321 aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct cgacctggct
    25381 gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc
    25441 tccggtctgg attacgccaa cttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc
    25501 gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca ccattttgtt
    25561 accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat cggtctgtca
    25621 aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga acgtctgacg
    25681 cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc tgtctcgatc
    25741 gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag tgccacaaca
    25801 acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga gtttctgcgc
    25861 gctgtgcgtc atcacaactg attaaaaggc aggaaccatg gagcgcaacg tcacgctcga
    25921 ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg cctttgggct
    25981 accaaaggcg gcttatctca atcccgcctg gtacggtgct attacgccgc aggatgcatg
    26041 gacctgggca tttcttgatc tcatcggcca ggtgaaattc ctcacgcttt ttgcgctgct
    26101 gtttggtgct ggactgcaaa tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt
    26161 aacgctgtta gtcttattgg gctttattca cggtttattg ttctgggacg gcgatattct
    26221 gctggcttac gggctggtgg gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt
    26281 aaaaagcctg tttaataccg gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt
    26341 attggggctg atttccgaca gccagaccag ccgcgcctgg acgccggatg catcggctat
    26401 tttgtatgaa aaatactgga agcttcacgg cggcgttgat gcgatcagta atcgtgccga
    26461 tggtgttggc aacagtttac tggcactggg cgcacagtat ggctggcaac tggctgggat
    26521 gatgctcatt ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt tcagcttacg
    26581 tcactatcgt cgtactggtt ttgtgctggt ggcgattggg gtgaccatta accttcctgc
    26641 catcgccctg caatggcagc tggactgggc gtatcgctgg tgtgccttct tacttcaaat
    26701 gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt tttatggctt
    26761 ctggccgcaa ttaagccgct ttaagctggt gctggcaatc gcctgcgtcg gacggatggc
    26821 gctgaccaac tatctattgc aaacgctgat ttgtaccacg cttttttacc acctcggttt
    26881 gtttatgcat tttgaccgcc tggagctgct ggcgtttgtt attccggtat ggctggcgaa
    26941 tatcctcttc tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg
    27001 gcgtcagtta actttgcgtg ctgccggacc ggcaatatct aaaacatcaa gataacgatc
    27061 tggatcacat tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg
    27121 aagtcctgta ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc
    27181 ataacaggta gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt
    27241 ggcaacggtt tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc
    27301 agtaatgaaa gccgtgagtg agctggatta tcggccaaac gccaatgccc aggcgctggc
    27361 aactcaggtt agcgacacca ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg
    27421 tgcgctggta aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg
    27481 caatagctat catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg
    27541 ttgtaatgcg ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat
    27601 ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg
    27661 cgtttgcctg gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg
    27721 tcatcaacgt attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa
    27781 agcaggctgg atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg
    27841 cactggtacg ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa
    27901 tctacaactt accgctgtat ttgcttataa cgacaatatg gccgctggcg cactgacagc
    27961 attaaaagat aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat
    28021 tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat
    28081 gactaaatta gccaccgaac tggccttgca gggggcagca ggcaatattg attctcgtgc
    28141 cagccactgt tttatgccga cgttagtgcg tcgtcattct gtcgcaacgc gccagaatgc
    28201 ggcggcgatc actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt
    28261 tcacagtatc ttaacaatgt gatagctatg attgcaccgt tttaacgttg taacccgtat
    28321 gtaacagtga ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt
    28381 ttctggagcg ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt
    28441 aacacttcat taactctact gccccgccga gcatttatct caagcactac cctgcataag
    28501 aaaaaccgga gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat
    28561 gttattcggt gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta
    28621 cgacgataac tttatgtctg tagtgcgcaa agctattgag caagatgcga aagccgcgcc
    28681 agatgttcag ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat
    28741 cgacgtattg ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc
    28801 tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa
    28861 agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgattc
    28921 caaagagtcc ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg
    28981 ttgggatctg aacaaagacg gtcagattca gttcgtacta ctgaaaggtg agccgggcca
    29041 tccggatgca gaagcacgta ccacttacgt gattaaagag ctgaacgata aaggcatcaa
    29101 aactgaacag ttacagttag ataccgcaat gtgggacacc gctcaggcga aagataagat
    29161 ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa gtggttattg ctaacaacga
    29221 tgcgatggca atgggcgcgg ttgaagcgct gaaagcacac aacaagtcca gcattccggt
    29281 gtttggcgtc gatgcgctgc cagaagctct ggcgctggtg aaatccggtg cactggcggg
    29341 caccgtactg aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct
    29401 ggccgatggc aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg
    29461 cgtaccttat gttggcgtag ataaagacaa cctggctgaa ttcagcaaga aataagactg
    29521 attgggcgca gtcgtttact gcgcccttat tgtatggcca acaaggtaaa attatatggt
    29581 cagctcaacg actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc
    29641 ctttcctggt gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca
    29701 tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat
    29761 ttatcaaaaa gactccggca ccattttatt ccagggtaaa gagatcgatt tccattccgc
    29821 caaagaggca ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca
    29881 acgttcggtg atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga
    29941 tcaggacaaa atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga
    30001 tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa
    30061 agcgttttcc tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga
    30121 aaaagaggtc aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat
    30181 tgtttatatc tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt
    30241 gcgcgacggt cagtggatcg ccaccgaacc gctggcagga ctgacgatgg acaagatcat
    30301 cgccatgatg gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa acaagccggg
    30361 cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt
    30421 ctcgtttgat ctgcataaag gggagatcct cggtattgcc gggctggtgg gggcgaaacg
    30481 taccgatatt gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt
    30541 gcacggcaaa cagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact
    30601 ggtaactgag gagcgccgct caacgggaat ttatgcctat ctggatattg gttttaactc
    30661 gttaatttcc aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat
    30721 gaaaagcgat acccagtggg tgattgattc gatgcgggta aaaacgccgg gtcatcggac
    30781 gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg attattggtc gctggctact
    30841 aacgcaacca gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa
    30901 gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga ttattattat
    30961 ctcctctgaa atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg
    31021 tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc
    31081 gtctttgcac ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct
    31141 tacttacctg aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat
    31201 tttccaggac ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc
    31261 ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgatct
    31321 ttctgctggt cgtcaggtag ggctggcggc agtggtggct gcgacattat tgcagtccat
    31381 ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct
    31441 gattgtctgt gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct
    31501 caacgtgacg ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gcatcaactc
    31561 gctctattac gactttgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac
    31621 ctttgctcag ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc
    31681 gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat
    31741 ttttgccatt ggcggtaacc cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa
    31801 cctgctgatg atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc
    31861 cggacgtatc ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc
    31921 ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt
    31981 gaccggggta attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgttaaccc
    32041 atactggcag tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact
    32101 gaaatacgcg cgtaagaagt gatgtgattg tggatattgc cggatgcgct gcatccggca
    32161 atcggtgcca aatgccggat gcgtctctgg ttttacaacg ttaccgggtg ttctttctcg
    32221 cctttcttaa acttcacttc cccgagctca atacaaccca ccgggcagac gtgaccacaa
    32281 agcagacaac ccacacattt ctcggtatta caatgcggtg tgcgggtttt ctcgctccat
    32341 tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac agcgtccaca gccaacacat
    32401 ttatcaagat tgatacgggg atagacaata taactgcggt ctaaatcttc cgccgggaca
    32461 atattgttat tcgccagacc taccatctcc tgcagcgaat caaatccctg atcggcgaga
    32521 taatgcgaca acccgcttgc catatcttcc actatccgat acccgtactg catgatgccg
    32581 gtggtcacct gtaacgttgc tgcgccgagc aataaaaact cagccgcatc ctcccaggtt
    32641 tcaatgccgc cgataccgct gattgggaaa tcgcgcagtt ctggatgggt gcgcatctgc
    32701 tgaatgaagc gcagggcgat cggttttacc gctttaccgg aatatccgga aatactcgat
    32761 tttccgttaa cgatcggcat accgattttc tgattaagat cgatattggt gatggattta
    32821 acggtgttaa tcgccgcaat gccatctgcg ccgccgcgct tcgccgccag cgccacttcg
    32881 cacatatcac cgatattcgg cgtcatcttc gccagcattg gcagcgtgga accccgtttc
    32941 actgcccgac aatatttttc taccagctcc gggctttgcc cgacatcgct acccatcgca
    33001 tgagaagtca tttgcggaca ggagaagtta cactcgatca tatccgcgcc agcttcttgc
    33061 accaggcgcg ccagctcctc ccattgctgc tcattttccc ccatgatcga agcgatcaat
    33121 actttgtccg ggtaatcttc cttcagccga cgcagggcgg ccagattctc ttccaacgga
    33181 tgttcagcaa tctgctccat atttttgaag ccgataaaac cggtatcttc tttcaccaga
    33241 tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc cgatcgtttt aaacaccaca
    33301 ccgccccaac ctgtgtcgta ggctttggca cacatctcat agcagttgcc taccggcgaa
    33361 gaagagaggc agaacgggtt gggaaacttc acgccgcaaa aagtaatcga aagatctttc
    33421 gttaacatga gcaagctccc tctaaatagt gatgaatcgc ctcggcggct tctttcccgg
    33481 ttttcacggc atagaccacg gttttgtcac cctcaacaat atcgcccgca gcaaagactt
    33541 gcggatcgcg ggtctgataa ttttgtgtat tgatagtgtt acgctgcggc tctaactccg
    33601 caaaggcatc cagtctggca tgctgaccga cggcgagaat aattttatct gccgccatcg
    33661 tcagttcgcc cggtaaccgt acatgcttaa acgtgacttt attgccttcc acggctactg
    33721 gcgtgaatcc atcgatgatc gaaacgccca gctcccgggc gctggcaaac tctttttcgc
    33781 ttgccggaaa ctcatctaac tcttcacgcg ctacgcaggt taccgcctga cagccgagaa
    33841 ctttcagcgt gctggctacg tccatcgcaa catcaccgcc gccgataatt aatgcgcttt
    33901 gtggaatgct gatatcgcct tgcgcctgtc gtgcacgttg caggaagtcc acggcaatct
    33961 caacgtcgct atgctcaaac agcaataacc cggaaccgct cgataacccc acggtgacca
    34021 gtaccgcgcg gttttctgct ttcagctgct caagggtgag tgtgttaccg acttcgttgt
    34081 tgcacttaat ggtcacgccc attttttcaa tacgggcgat ctctgcatcc agcactgatt
    34141 gcggtaaccg gaattgcgga ataccgttac gcaaccagcc accggggtgc gcttctttct
    34201 cataaatcgt gacgtcataa ccctggtttg tcagtgttac actggcctgc aatccggcag
    34261 gacctgcgcc aataatcgcg actttgccga gcgttttagt accgggctga taaatttcca
    34321 tcccggtttg ttgttcaaaa tcagtaacaa aacgctgtaa gcggccgata tcaatgggag
    34381 catcaacacc ggcacgggta caaccgcttt gacataattt ctccgtcggg cacactctgg
    34441 cacaaacggc accgagggca ttattttcgc gaattgtctc ggcagcgcct ttaaaattac
    34501 gaaagtagat tgagcgaata aatttccccg gatcggtctg tgccgggcaa gcctgactac
    34561 agggagcgtc atgacataat aaacagcgag aggcttcttt aatcgccagt aaaggcgtaa
    34621 aagccggagt gagttcatcc agataatttt gttgcggcat gtgatcttcc ttaatgctat
    34681 gagtaattaa ggcgaacgct aaatatactc gtcatacttc aagttgcatg tgctgcgtct
    34741 gcgttcgctc accccagtca cttacttatg taagctcctg gagactcact cacttgtcgc
    34801 cttcctgcaa ctcgaattat ttagagtata aatattgcgt gctgatatta aggctatgcg
    34861 cgtggggaaa tttatactgt gaaaggattc acaaatagct gcggttcaga atgttatatc
    34921 cgcagcttat tgagtgaagt aataattatt ctcaataatt aacagaaaag agaaaatatt
    34981 aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag ccgatatatg
    35041 ccaccatata agcgaaaaag agtccgggat tttttgctcg ccagacatac accggttccg
    35101 gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat gcttcgcgga
    35161 aacaaaatat cgggataagg aaaaaaacgg caacgacaaa aaactgctgt acatccataa
    35221 ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtgacgg cgggataccc
    35281 ctgcgcatct tccggcacct ggtgcatagc cggaataggg accagcggcc ctaaaaaacg
    35341 cggttcacgt ttaaaaatat aaaggtcagc cagcgctccg aaacgggcgg caaattcgcg
    35401 aatacgtact gacagcatat ctttcggtga cggtacggca taacactgag cctgaatccc
    35461 catatgcagc gcaataaata atgcccgctc acagtggaaa cgttgggtga taataatgaa
    35521 atcattagta tcgaaaactt tgcgtgtacg cactatggaa tccagcgtac gaaagcctgc
    35581 gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt tgcgcatggt
    35641 catcggctca ttataacttt gcaatgcgtt atcgccgctc agtaatagat aatttacctt
    35701 accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact gattaattac
    35761 gccagtacga taatattttg ctgttccgag caccacaccg acctggcggt aggggagatc
    35821 ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat cgaggccgag
    35881 cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc gctttaacat
    35941 gcaggagact cgatctaagg gaaatttcca tcaggctact ttacccgctg gttttgcgca
    36001 agaaacggca aactgtttgc cgggattttc gacaccagga gagggctggc aagccggaca
    36061 aggcgttcac gccgcatccg gcaccaggct taagcgagaa gcacgcggtc gatactgtga
    36121 cagccgagcg ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg cgcatcggct
    36181 ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag attcaacaga
    36241 attaacgctc cctgcaacgg tggcagagtc gggttgaatg cggcgttttc agcgtagctg
    36301 ccactgaaaa tacgaccgtc tttacattcc agcgcgacac cgcttggcga cttactgtaa
    36361 ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag aaagcgcatc accagtcagc
    36421 gcatagccgt gatcctgctc gtccatcagc agcgttttaa tctccagatc tttcggccca
    36481 aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcag atgaatacgc
    36541 agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca aggcgtgtag
    36601 ttaacggtga tggctgcaag cgcttcttca ccactcaacc aggcgtggct gatcgcgctt
    36661 tgttcggcat gaacggtttg ctgcattgtc gcaccaataa attccatatt ggcaccgaaa
    36721 taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga caatggcgta
    36781 cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc gtccagcccc
    36841 gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa gtacttgtct
    36901 gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa agcggtttga
    36961 aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt tacgaacccg
    37021 ttatggcttt atatgtgatc tgaatctcat taattatgca acgcgtgaaa cttacatcaa
    37081 aaatgcgcga cgcatcgcaa attttagccc attactgcca gaataatcgg gaaaaggaac
    37141 ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ctagcgaact aaatgcacct
    37201 tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc agtccccatt
    37261 gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt atggccaaat
    37321 accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg aataccgcca
    37381 atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag gattgacgcg
    37441 gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac catcgcaacc
    37501 acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat ctggtgtagc
    37561 tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc gtttagcact
    37621 tcgctacctt taaagtagct gtcgtaagag atgccggtca gcattaaaaa aggaatgatc
    37681 accaccattg ctaccagcaa cgggttaagc aggggaaact tataccgtgc cgccagtttg
    37741 cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc accagatatt cgccatcatt
    37801 cttctgatcc tttctgacct acgactttac gttcaccgtg taccagttgt gaactccagc
    37861 tcaccaccag aaaaaccacc agcgtactga cagcacagga aaccactacc gggccaaact
    37921 gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca aacaatagcg
    37981 ccatatagcg aatcagtacg tagcaccccg gattgaccca tttcgccgga agaatttgca
    38041 aggccagcag gacaaacagg atcagcatcc cgatgatgct gcccggaatg gttaccggta
    38101 gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg aaagcgcgta
    38161 aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat gagatggatt
    38221 catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca gcgcttattt
    38281 tttcgctccc cggcattacg caatgcgcaa cgaactcagg catacatcaa gcatagagag
    38341 ggattgcagt ttgcagcaat ccctctttgt agacctttaa tccgctgacc ataggcgcta
    38401 acatagcgcc tcattatttt gcgggtgatg atatgcgtgt gttactggca ccgatggagg
    38461 gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt taacgactac gatctgtgca
    38521 tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt catcgcattt
    38581 gccctgagct acaaaacgcc agccggacac catctggtac gctggtgcgc gtgcagttgt
    38641 taggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggag ttaggttcct
    38701 ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt taacggtagc ggcggcggcg
    38761 cgacgttact caaagatcct gaactcatct accagggtgc aaaagcgatg cgtgaagctg
    38821 taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg ctgggacagc ggtgagaaga
    38881 aatttgaaat cgccgatgcg gttcaacagg ctggcgctac ggagctggtg gtgcatgggc
    38941 ggacgaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt ggcgatattc
    39001 gccagcggct gaatattccg gtgattgcca acggtgaaat ctgggactgg cagagcgcgc
    39061 aacaatgcat ggcgatcagc ggctgcgacg cagtgatgat tggtcgcggg gcgctcaata
    39121 ttcccaacct gagccgggta gtaaaatata acgaaccgcg aatgccgtgg ccggaggtgg
    39181 ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg cgataccggg ttatatcacg
    39241 ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa gcaacgggat
    39301 tatttcagca tgttcgggtg ttgaataatt cccctgatat tgcaagggct attcaggcaa
    39361 ttgatatcga taagctgcga taatagtgga atcgcagctt atcagagaca atgaatcgct
    39421 tattcagctt catcatcttg ttgcgctcct ttcatga
//
LOCUS       DAZFYC010000035        39397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFYC010000035 DAZFYC010000000
VERSION     DAZFYC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.035"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..784)
                     /gene="aqpZ"
                     /locus_tag="ACHKW5_002000"
     CDS             complement(89..784)
                     /gene="aqpZ"
                     /locus_tag="ACHKW5_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO7511406.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGDWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1210..2868
                     /gene="ybjD"
                     /locus_tag="ACHKW5_002001"
     CDS             1210..2868
                     /gene="ybjD"
                     /locus_tag="ACHKW5_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO7511407.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2865..3821)
                     /gene="ybjX"
                     /locus_tag="ACHKW5_002002"
     CDS             complement(2865..3821)
                     /gene="ybjX"
                     /locus_tag="ACHKW5_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO7511408.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3972..5087
                     /gene="macA"
                     /locus_tag="ACHKW5_002003"
     CDS             3972..5087
                     /gene="macA"
                     /locus_tag="ACHKW5_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO7511409.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5084..7030
                     /gene="macB"
                     /locus_tag="ACHKW5_002004"
     CDS             5084..7030
                     /gene="macB"
                     /locus_tag="ACHKW5_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO7511410.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAETEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVIERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNASRLDPVDALARE"
     gene            complement(7103..7327)
                     /gene="cspD"
                     /locus_tag="ACHKW5_002005"
     CDS             complement(7103..7327)
                     /gene="cspD"
                     /locus_tag="ACHKW5_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO7511411.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7477..7551
                     /gene="yljB"
                     /locus_tag="ACHKW5_002006"
     CDS             7477..7551
                     /gene="yljB"
                     /locus_tag="ACHKW5_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO7511412.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7650..7970
                     /gene="clpS"
                     /locus_tag="ACHKW5_002007"
     CDS             7650..7970
                     /gene="clpS"
                     /locus_tag="ACHKW5_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO7511413.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8001..10277
                     /gene="clpA"
                     /locus_tag="ACHKW5_002008"
     CDS             8001..10277
                     /gene="clpA"
                     /locus_tag="ACHKW5_002008"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO7511414.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10621..10708)
                     /locus_tag="ACHKW5_002009"
     tRNA            complement(10621..10708)
                     /locus_tag="ACHKW5_002009"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10672..10674),aa:Ser,seq:gga)
     gene            complement(10962..11180)
                     /gene="infA"
                     /locus_tag="ACHKW5_002010"
     CDS             complement(10962..11180)
                     /gene="infA"
                     /locus_tag="ACHKW5_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO7511415.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11465..12169)
                     /gene="aat"
                     /locus_tag="ACHKW5_002011"
     CDS             complement(11465..12169)
                     /gene="aat"
                     /locus_tag="ACHKW5_002011"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO7511416.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCSESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12211..13932)
                     /gene="cydC"
                     /locus_tag="ACHKW5_002012"
     CDS             complement(12211..13932)
                     /gene="cydC"
                     /locus_tag="ACHKW5_002012"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO7511417.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13933..15699)
                     /gene="cydD"
                     /locus_tag="ACHKW5_002013"
     CDS             complement(13933..15699)
                     /gene="cydD"
                     /locus_tag="ACHKW5_002013"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO7511418.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15822..16787)
                     /gene="trxB"
                     /locus_tag="ACHKW5_002014"
     CDS             complement(15822..16787)
                     /gene="trxB"
                     /locus_tag="ACHKW5_002014"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO7511419.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17331..17825
                     /gene="lrp"
                     /locus_tag="ACHKW5_002015"
     CDS             17331..17825
                     /gene="lrp"
                     /locus_tag="ACHKW5_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO7511420.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17960..22027
                     /gene="ftsK"
                     /locus_tag="ACHKW5_002016"
     CDS             17960..22027
                     /gene="ftsK"
                     /locus_tag="ACHKW5_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO7511421.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22182..22793
                     /gene="lolA"
                     /locus_tag="ACHKW5_002017"
     CDS             22182..22793
                     /gene="lolA"
                     /locus_tag="ACHKW5_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO7511422.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22804..24147
                     /gene="rarA"
                     /locus_tag="ACHKW5_002018"
                     /gene_synonym="mgsA"
     CDS             22804..24147
                     /gene="rarA"
                     /locus_tag="ACHKW5_002018"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO7511423.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24238..25530
                     /gene="serS"
                     /locus_tag="ACHKW5_002019"
     CDS             24238..25530
                     /gene="serS"
                     /locus_tag="ACHKW5_002019"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO7511424.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25769..28213
                     /gene="dmsA"
                     /locus_tag="ACHKW5_002020"
     CDS             25769..28213
                     /gene="dmsA"
                     /locus_tag="ACHKW5_002020"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO7511425.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28224..28841
                     /gene="dmsB"
                     /locus_tag="ACHKW5_002021"
     CDS             28224..28841
                     /gene="dmsB"
                     /locus_tag="ACHKW5_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO7511426.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28843..29706
                     /gene="dmsC"
                     /locus_tag="ACHKW5_002022"
     CDS             28843..29706
                     /gene="dmsC"
                     /locus_tag="ACHKW5_002022"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO7511427.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29742..30368)
                     /gene="ycaC"
                     /locus_tag="ACHKW5_002023"
     CDS             complement(29742..30368)
                     /gene="ycaC"
                     /locus_tag="ACHKW5_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO7511428.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30683..31831
                     /gene="ycaD"
                     /locus_tag="ACHKW5_002024"
     CDS             30683..31831
                     /gene="ycaD"
                     /locus_tag="ACHKW5_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7511429.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTLRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32041..33471
                     /gene="ycaM"
                     /locus_tag="ACHKW5_002025"
     CDS             32041..33471
                     /gene="ycaM"
                     /locus_tag="ACHKW5_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7511430.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMKNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33472..34380)
                     /gene="ycaN"
                     /locus_tag="ACHKW5_002026"
     CDS             complement(33472..34380)
                     /gene="ycaN"
                     /locus_tag="ACHKW5_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7511431.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            34480..35070
                     /gene="ycaK"
                     /locus_tag="ACHKW5_002027"
     CDS             34480..35070
                     /gene="ycaK"
                     /locus_tag="ACHKW5_002027"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO7511432.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(35152..35892)
                     /gene="pflA"
                     /locus_tag="ACHKW5_002028"
     CDS             complement(35152..35892)
                     /gene="pflA"
                     /locus_tag="ACHKW5_002028"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO7511433.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASSGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(36084..38366)
                     /gene="pflB"
                     /locus_tag="ACHKW5_002029"
     CDS             complement(36084..38366)
                     /gene="pflB"
                     /locus_tag="ACHKW5_002029"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7511434.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMATKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38421..39278)
                     /gene="focA"
                     /locus_tag="ACHKW5_002030"
     CDS             complement(38421..39278)
                     /gene="focA"
                     /locus_tag="ACHKW5_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO7511435.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9790 a         9608 c        10418 g         9581 t
ORIGIN      
        1 tcatgaaagg agcgcaacaa gatgatgaat caacatataa caacatctta aaaaaaggcc
       61 tgacattacg ccaggccttc tgcgttaatt aatcacgctt ttccagcagg gtccggtaaa
      121 tcagaccacc gataatgccg ccgacaattg gcaccaccca gaagaaccac agttgttcta
      181 atgcccagtc gccctggaag atagcaaccg cggtgctgcg cgccgggtta acagaagtgt
      241 tagtcaccgg aatactaatt aagtgaatca gggttaaggc cagaccaata gcgatcggcg
      301 caaaacctgc cggcgcgaat ttgtcggttg cgccgtggat caccaacagg aaacctgcac
      361 tcaataccag ttcaactacc agcgcggaaa gcatggaata accgcctggt gaatgctcgc
      421 cataaccgtt agaagcaaaa ccgctggctg ccgcgtcaaa acccgtttta ccactggcaa
      481 ttaaatacag cagcgccgct gcaacaatac cgccgacaac ctgggcaatt acgtagccaa
      541 cgacttcttt tgccggaaaa cgtccgccag cccataaacc aatagtgacc gccgggttaa
      601 aatgaccacc agaaatatga ccaacagcaa aggccatcgt cagaacggtc agaccgaacg
      661 ccaacgccac gccggcaaaa ccaatgccta attccgggaa gcctgcggcc agtacagcac
      721 taccacagcc accaaaaaca agccagaaag taccaaaaca ttcagctgct aattttctga
      781 acatatccac ctcaattaaa aattgaccct gtgaaaaata tggtcgtttt atagggtcgt
      841 cgtaaaaaag tgacgacgga aataatgcgc ggctatttta aaaacgaagg cgagtcattc
      901 accagataaa taaatccagt aaatttgatt tagggcaaca gcgggttgcc ccatatagtc
      961 atttgtctga ttgacagtgt agtgcaagca aaagatttaa tcctttaggc gtaataaaaa
     1021 ataatttatc atgctaatta tttgattttg ttgtttttgc agacttatca gcaagaggga
     1081 gtataacgcg attattcgct catttttcag acatttgcca tgcttaaatg tgatgtcatc
     1141 acgtattagc aaggcctttc ccgttatact gccagcgtaa aggataagtc acatatttct
     1201 ggaggggata tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg
     1261 tcgttgatgc tggaacaaaa caacgtcctg attggggaga acgcgtgggg taaatccagc
     1321 ttgctggacg ccttaaccct gctgctatcg ccagaatcag atctctacca ttttgagcgc
     1381 gacgatttct ggttcccgcc gggagatatc aacgggcgag aacatcatct gcatattatt
     1441 ttgaccttcc gcgaatcgct gccaggccga catcgggttc gccgttatcg gccgctggaa
     1501 gcgtgctgga cgccatgcac cgatggctat caccgtattt tttatcgtct ggaaggggag
     1561 agtgcggaag acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg
     1621 attgatgtcg aggatattaa cgatcaggca cgccatctgg tgcgtttaat gccggtgctg
     1681 cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc
     1741 aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg
     1801 caaaatctct ctgatggaca gattcgtcag ggactttccg caatggtgca gttgcttgag
     1861 cattatttct ctgagcaggg ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc
     1921 agcaatgagc aacgaagctg gcgctatctg gatatcatca accggatgat tgaccgacct
     1981 ggagggcgct cgtatcgggt tattttgctc ggcctgtttg caactttatt gcaggcaaaa
     2041 ggcacattgc gactggataa agacgcccgt ccattgttgc tgattgaaga tccagaaacc
     2101 cgtttacacc ccattatgct ttcagttgcc tggcatctgt tgaatcttct gccattgcag
     2161 cgcattgcca ccaccaactc gggtgagttg ctttcgttaa ctccagtgga acatgtttgc
     2221 cgcctggtgc gtgaatcttc tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc
     2281 accgaagaca gccgccgcat ctcttttcac attcgtttta atcgtccgtc atcgctgttt
     2341 gctcgctgct ggttgctggt ggaaggggaa acagaaacct gggttatcaa cgaactggcg
     2401 cgccagtgtg gacaccattt cgatgccgaa gggatcaagg tcattgagtt tgcccagtcc
     2461 gggctaaagc cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc
     2521 gatggcgatg aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataacgat
     2581 cgggaagccg aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg
     2641 tatcgccagg gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg
     2701 atgaatctgc gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc
     2761 attgaagtgg caatggaggc cggacgtcgt ggtgtggact ccgtaccgac gctgctgaaa
     2821 aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc
     2881 gtggccattt gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt
     2941 tttttactgg cgatttcggc aatctctttt cgtgctatct gtgctggaag gcggtaatgg
     3001 cgttcagcat cacatacgcc gccaaccgat tcccagaaag cgttgtaatc ggcatggatc
     3061 ttgccttctt tatcacgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca
     3121 ataatctgct ctacctgcaa acgttgggca aacagacagg ccgcttccat cacgaggcgt
     3181 ttgggaaata gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc
     3241 cattttgcgc cttgcagtcc gccaataaac atcgttcttt tcccctgata ttcacacagg
     3301 gtaaacgtga tctctgccag aggaatacct tcgctgttgc gaaacaggat tgtgctgtca
     3361 ccttctttat ccattgagat catcatggtc agctcaagcg tgaactgctc gccgtttttg
     3421 ccttccagct tcgccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc
     3481 gacatacatc cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg
     3541 ctaagattcg ccgcaaggta agggcgatgc agacgcacag gcagtcgcgg ttggcgcgtt
     3601 aacaacacat tgagattagg ccagtgggaa agttcgttca tccactcaac gcttaaacgc
     3661 ggcataatca acgagcgcag caaaaatttc tggcgaaaac tacggcgatg ccagaattta
     3721 cccggccgac actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca
     3781 gatggcgtaa aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat
     3841 gacgtcgatt tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaacgtaa
     3901 attttagggt tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat
     3961 tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga
     4021 tagtcatcgc cggactgatt acgttatgga gaattcttaa cgcacccgtg ccgacttatc
     4081 agacactgat tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg
     4141 acgcgctgcg taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa actctgtcgg
     4201 tggcgattgg cgataaagta aaaaaagacc agcttttagg ggttattgat cctgaacagg
     4261 ctgaaaacca gattaaggag gtcgaagcaa cgctgatgga gctacgtgcg cagcggcagc
     4321 aggcggaagc ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac
     4381 aaacgcaggc ggtttcacag caggatctcg acaccgccgc gacggagatg gctgtgaaac
     4441 aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcttct ctcgatacgg
     4501 ctaaaaccaa tctcgattac actcgcatcg ttgccccgat ggccggggaa gtcacgcaaa
     4561 tcaccactct gcaaggccag acggtgattg ccgcacaaca agcaccgaac attctgacgc
     4621 tggcagatat gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc
     4681 tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga tccactgacg cgctacgagg
     4741 ggcaaatcaa ggatgtacta ccgacgccgg aaaaggttaa cgacgctatt ttctattacg
     4801 cccgttttga agtccccaac cccaatggtt tgctgcggct ggatatgact gcgcaagtgc
     4861 atattcagct caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg ttaggcgatc
     4921 cggttggcga taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg
     4981 aagtgacgat tggcgcacgt aacgataccg atgttgagat tgtcaaaggg cttgaagcgg
     5041 gcgatgaagt ggtgattggt gaggccaaac caggagctgc acaatgacgc ctttgctcga
     5101 attaaaggat attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg
     5161 catcaccctc gatatttatg ccggtgagat ggtagcgatt gttggcgctt caggttccgg
     5221 taaatcgacc ctgatgaata ttctcggctg tctggataag gccaccagcg gcacctatcg
     5281 cgtcgccggt caggatgttg ccacactgga cgccgatgcg ctggcgcaac tgcgccgcga
     5341 gcatttcggc tttattttcc agcgttacca tttactttcg catttaaccg ccgagcagaa
     5401 cgttgaagta cccgccgtct atgccggtct tgagcggaaa cagcggctgc ttcgcgccca
     5461 ggggttactg caacgactgg ggctggaaga ccgtacagag tattatccgg cacagctttc
     5521 gggtggtcag caacagcgcg tcagcatcgc gcgggcattg atgaacggcg gtcaggtaat
     5581 tcttgccgat gaaccaaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat
     5641 cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc gtcacccacg atccgcaggt
     5701 cgctgctcag gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc
     5761 cgccattgaa aaagtgaatg ttgctggcgg gacggagcct gttgtcaaca cggtgtctgg
     5821 ctggcggcag tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcgctggc
     5881 agcgaataaa atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt
     5941 ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc
     6001 tattggtacg aatactattg atgtctatcc cgggaaagat tttggcgatg acgatccgca
     6061 atatcagcag gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc
     6121 ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc
     6181 tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga
     6241 aggaaacacc tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag
     6301 taatactcgc cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt
     6361 cggcaatatg cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag
     6421 cagtaaagtg ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca
     6481 gtcgtggctg aactccatta ctgtcagggt aaaagaagga tttgacagcg ccgagacgga
     6541 acagcaactc acgcgtttac tttcactgcg ccacggaaag aaggatttct ttacctggaa
     6601 catggacggc gtcttgaaaa ctgttgaaaa gaccacacgt actttacaac tgtttctgac
     6661 gctggtggcg gtgatttcgc tggtggtggg cggtattggt gtaatgaata ttatgctggt
     6721 gtcagtgatc gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag
     6781 cgatgttttg caacagttcc tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt
     6841 gggaataact ctgtcactgt taattgcttt caccttgcag cttttcttac ccggctggga
     6901 gattggtttt tcaccgttgg cactgctgct ggcgtttctc tgctcgacgg ccaccgggat
     6961 tttatttgga tggttacccg cacgaaatgc gtcacgactg gatccagtag atgctctggc
     7021 acgagagtaa tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga
     7081 tgtacacaat gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa
     7141 cactggcgtg attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt
     7201 ttagcgttct gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc
     7261 cgccttcagg gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct
     7321 tttccatgct tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc
     7381 agagaggggg ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg
     7441 agccgtcaag cgtttgacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt
     7501 aacgctattg acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttatct
     7561 atcatgtgga gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg
     7621 ataaccgtaa ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg
     7681 atcaactggc ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga
     7741 tattagtcaa tgatgattac actccgatgg agtttgttat tgacgtgtta caaaaattct
     7801 tttcttatga tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg
     7861 ccatttgcgg agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt
     7921 acgcgaggga gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat
     7981 aaaaattggg ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt
     8041 tcgccagagc gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc
     8101 tgctcagtaa cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc
     8161 tccgtcagga actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag
     8221 aggagcgcga cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc
     8281 atgtccaatc ctccggtcgc aatgaggtaa ccggtgcaaa cgttctggtc gctatcttta
     8341 gcgaacagga gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg
     8401 tggtgaactt tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg
     8461 gcagccagcc aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga
     8521 cgaacctgaa tcagcttgcg cgcgtgggcg gaatcgaccc actgattggt cgtgagaagg
     8581 agctggagcg tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg
     8641 gggaatctgg tgtcggtaaa accgcgattg cggaaggtct tgcctggcga attgttcagg
     8701 gcgatgtgcc ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt
     8761 tagcgggcac taaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc
     8821 tggagcagga cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg
     8881 gggcagcgtc tggtggccag gtcgatgcgg ctaacctgat caaaccgttg ctctccagcg
     8941 gtaaaattcg cgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag
     9001 accgtgctct ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtcg atcgaagaaa
     9061 ctgttcaaat catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata
     9121 ccgcaaaagc ggtgcgtgca gcggtagagc tggcggtgaa atacattaac gatcgtcatc
     9181 tgccggataa agccattgac gttatcgacg aagcgggcgc tcgcgcacgc ctgatgccgg
     9241 taagcaaacg caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg
     9301 cgcgcattcc agagaagagt gtttctcaga gtgaccgcga taccctgaaa aacctcggcg
     9361 atcgcctgaa aatgctggtc ttcggtcagg ataaagccat tgaggcgctg actgaagcca
     9421 ttaagatggc gcgtgcaggt ttaggtcacg aacataaacc ggttggttcg ttcctgtttg
     9481 ccggccctac cggggtcggg aaaacagagg tgacggtaca gctttcgaaa gcgttgggca
     9541 ttgagctgct gcgctttgat atgtccgagt atatggaacg ccataccgtc agccgtctga
     9601 ttggtgcgcc tccgggatac gttggttttg atcagggagg tttgctgact gatgcggtca
     9661 tcaagcatcc acatgcggtg ctgttgctgg acgaaatcga gaaagcgcat ccggacgtgt
     9721 tcaatattct gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag
     9781 cggacttccg taacgtggtg ctggtgatga ccaccaacgc cggggtacgt gaaactgagc
     9841 gtaaatccat tggtcttatc caccaggata acagcaccga tgcgatggag gagatcaaga
     9901 agatctttac accggaattc cgtaaccgtc tcgacaacat tatctggttt gatcatctgt
     9961 caaccgacgt gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagctgg
    10021 atcagaaagg tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag
    10081 gttacgaccg ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa
    10141 aaccgctcgc caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg
    10201 cgctggataa agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg
    10261 cggaagcagc gcattaatct gattgttagg taggttggtc aagtccgtaa tctcgaaaga
    10321 ggttacggac tttttgttta tggggtggag gaggttcaga cccttttttt aatgatgatg
    10381 gtaagttgtt gataattagt gctgcgggaa ggtaaggata aaaaagggtg ctgcaggaga
    10441 atgggatggt tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgcca
    10501 tacaggcagc ttagaaatcg acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg
    10561 tacagacaga taaaatgcga aaaaaaagct cgcactttcg tacgagctct tctttaaata
    10621 tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt
    10681 gctccttcag ccactcggac acctcaccaa attgttttgt cgcctgacct catgggtggc
    10741 aacggggcgc tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt
    10801 attgtttgct catactgtaa acaagttgtg cagtatatct acatcgagac aagttacgga
    10861 cttatacttc caaagtactt catacatatc acaaaataaa aaggccggtt aaaccgacct
    10921 tttactcgtt ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca
    10981 atgcggcctt tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg
    11041 atgcggatgt agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt
    11101 tctaactcta cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca
    11161 atattgtctt ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca
    11221 agataatgcc gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg
    11281 ttcggcacat tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg
    11341 agcggtgaga taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt
    11401 aatgagcgta accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa
    11461 acattcattc ttgtggtgaa aacaagcatc gtggtaccca gaaattattc ggcaatcgtc
    11521 cgaggcgcat ttgattgaga taattaaggt aatcccggcg gggaatttcg caggcaccaa
    11581 gcgatgctgt gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa
    11641 attcctcaca gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg
    11701 actcgctaca aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt
    11761 cacgccagac ttcaatggag tgggcatggc cgagttcgtg aaggcgatgg taggcttcga
    11821 ccacgccacg cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct
    11881 gaccaaaagc gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac
    11941 tacggctgat atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga
    12001 ggatggggtc gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc
    12061 gcgcagggct aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt
    12121 ccggggaagg gaaggctatt gaatggcgag aaagctgaac caggcgcatg accgcaaaac
    12181 tccacgcaag tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg
    12241 cccctgtctg gcaagcagct ctgcgtgagt accttgctca ataatttgcc cgttgtccat
    12301 cactattatt tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt
    12361 tttctcacgc atcatttctg caagcaattc aaggatctgg ctttcggttg tggcatctaa
    12421 gccttcggta ggttcatcca gcaacaccag tggcgcatca tgtaacagcg cacgggcgat
    12481 agccagacgg cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt
    12541 gagacctgca tcctcgagca gcttctccag gccaacgcga cgcaagctct ccgccagagc
    12601 ctcatcacta ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg cgctaaacag
    12661 atgcactcgc tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc
    12721 tatggggcta tcgttaagca aaatctcgcc ctgttgcggg tcccaagcgc gggtcagcag
    12781 ttgtaacagt gttgatttgc cgcatccggt tcgcccgaga atcgcaatat gttccccggc
    12841 gtttacctga agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg
    12901 aacatcccgt aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat cagggaaggt
    12961 gacttccggt ttttgatccg ttaaatcagt gatccgaacg gcagaggcaa tgacttgccc
    13021 cagatgctga aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcgcagaa
    13081 gacaaacagg gcaattaacg cgccgggttg agcattgccg ccaacgccgc cagacgccat
    13141 ccacagcatc aggatcaccg ctaacgcgcc aatgagtagc attatcgctt gcgacaatgc
    13201 ggtcagttca gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg
    13261 cgtgcgataa cgatcgctgg caccaaaaat ggtcagctca gcttgccctt gcagccaggc
    13321 cgtcagttgt tggcgatact gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc
    13381 cgcacgataa aacagcggtg gcatcaggaa aagcgtcagt aacataatgc cgcccagcgt
    13441 aaaggcgagg gtgaaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa
    13501 agcgcccacc agcggcgaga taacgcgcag ataaagatga tcgagcgtat caacatccgc
    13561 caccacgcga ttgagcaatt cgccctgacg atagcgcgcc agtccggcag gggagagggg
    13621 cagcaatttg ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg
    13681 acttaccaga cgttcaaaat aacgcccggc agtacgggtg attgctgcgc cacgcacgcc
    13741 cgcagcgggt agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc
    13801 cgagaggaac cagccggaaa gtgtcaacag accgatactg gcgagcagcg tgacaattgc
    13861 cagcacgata ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag
    13921 caaagcgcgc atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc
    13981 agccacactt aattccgcgt aacgtccttg ctcaataatc cggccatcct gcataaccca
    14041 aatgacatcc cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg
    14101 cagagaggcg gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc
    14161 agcgggttca tccaacagta atagcgaaca gggatttagt aacgcacggg ccaccgccac
    14221 gcgctgcgcc tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg tatcaacgcc
    14281 ttgtggcagg agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc
    14341 ttgttcgctg gcatcaggtc gcgccagtag tacgttatcc cgcaatgttg ctgccggtaa
    14401 ttgtgggttt tgcccaaccc aggagagatg tttacgccat gattctggtg ataaatcgcg
    14461 taattctatc ccgttgattc gtaatgatcc ctgatatgaa agaaaaccag aaagcgcgtt
    14521 cagcagtgag cttttacctg aaccgctgcg accaaccaac actgcacgtt ggcctgctgg
    14581 caaagtaaag ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc
    14641 ctcggcctca atggtcaccg gatcggtcga tgctaattcc gcctcaccgc gctgcggatg
    14701 ggcgagcggg gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt
    14761 agcatgataa aacgtaccga gatcgcgcaa tggctggaaa aactctggcg caaggatcag
    14821 ggccagaaaa cccgcagcca gcgtcacacc ggtatcgtag tgaccaaaat ccagctcgcc
    14881 gagataggaa aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc
    14941 gagaatgccg gaggataaaa acgccagccg tagcacttcc attgtccgtt ggcggaaatc
    15001 ttccgaagca gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt
    15061 ttccatgccg cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt
    15121 acgccggtta gcatcggcag cccccatccc aaccagcgcc ataaacaacg gaattaacgg
    15181 tgctgtgccc agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat
    15241 cagcaacggc accgacactg ccagcgccat ttgcggcagg tagcgtgcat agtaatcatg
    15301 catatcgtca atttgctcga gtaccaacgt cgcccagctc cccgcaggtt taccctgaat
    15361 ccacgctggc cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat
    15421 atgctgcccg gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcagtac
    15481 aaaggtcaga accagtaacg taaagggaag cagcagggct tcacggggaa tattctccat
    15541 aatcatatgt tgcagaattc gcgccatgaa ccaggcctgg gcaataatca atatgccgct
    15601 tacaaagccc agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt
    15661 taaccagcgg gttaactctt tttgacggga tttattcatt gcacgcttag caggtgagtt
    15721 atcagaatta tttgcagagc aatgttacaa cggggaaaaa ataaaggcga cccatagtcg
    15781 catggtgtcg ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa
    15841 ccatcgaggt agcgttccgc atcaagtgct gccatgcagc ctgtaccggc cgaagtaatg
    15901 gcctgacgat aaatatgatc catcacgtca cccgcggcaa agacgccagg gatgctggtc
    15961 tgggtggcat taccatgaat acccgactgc actttgatgt agccgttttc cagttccagc
    16021 tgcccttcga aaatggcagt attcgggctg tgaccgatag caacaaacag accagcaacg
    16081 tcgagtgact cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacaccc
    16141 atttgatcgc cggtcacttc ttccagcgta cggttggtgt gcagaatgat gttgccgttc
    16201 tccactttat ccatcagacg cttaataagg attttttccg cgcggaaacc gtcacggcgg
    16261 tgaatcagat gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta
    16321 ttgccgccgc cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggttgca
    16381 caagcagaaa ccccacggcc tttaaaggct tcttcagagg gcaggccgag atagcgtgca
    16441 gaagctccgg tggcaataat cagcgcgtcg caagtgtatt cgccgttatc gccattcaga
    16501 cggaacggac ggttttgcag atccaccttg ttgatatgat caaaaatgat ctcagtttca
    16561 aacttggtgg catgttcgtg catgcgctcc attaataacg gaccggtcag atcgtttgga
    16621 tcgccaggcc agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg
    16681 gtaatcagca caggttgcag gttggcgcgc gccgcgtaga cagcagcggt gtatcccgcc
    16741 gggcctgaac ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag
    16801 ttgttggcag acaatgggca ggattgtagg gaatttacag acgtaaaaaa agagtatgac
    16861 gattttgtta acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca
    16921 ttatcacctc tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc
    16981 ccctgttaat gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc
    17041 ctggtgtttt gcgaaaacat tcgaggaaga aaaaaacagt attcttatat gcgcataacc
    17101 atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta cgtatggcgt ggacagacgc
    17161 cattcgtgat gtcgatagct gccacaaggc aacggtcttc tcaccgtaga cccaggcatt
    17221 gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg gtagcgactc tgaacagtga
    17281 tgtttcaggg tcagacagga gtagggaagg aatacagaga gacaataata atggtagata
    17341 gcaagaagcg ccctggcaaa gatctcgacc gtatcgatcg taacattctt aatgagttgc
    17401 aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg tgtgggactt tccccaacgc
    17461 cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt tattcagggc tatacggcgc
    17521 tgcttaaccc ccattatctg gatgcatcac ttctggtatt cgttgagatt actctgaatc
    17581 gtggcgcacc ggatgtgttt gaacaattca ataccgctgt acaaaaactt gaagaaattc
    17641 aggagtgtca tttagtatct ggtgatttcg actacctgtt gaaaacacgc gtgccggata
    17701 tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg tctgcctggc gtcaatgaca
    17761 cacggacata cgttgttatg gaagaagtca agcagagtaa tcgtctggtt attaagacgc
    17821 gctaacacgg aacaggtgca aaatcggcgt attttgatta cactcctgtt aatccataca
    17881 gcaacagtac tggggtaacc tggtactgtt gtccgtttta gcatcgggca ggaaaagcct
    17941 gtaacctgga gagcctttct tgagccagga atacactgaa gacaaagaag tcacattgac
    18001 aaagttaagt agcggccgtc gccttctgga agcgttgctg atccttattg tcctgtttgc
    18061 cgtctggttg atggctgcct tactaagctt taacccttcg gaccccagct ggtcgcaaac
    18121 ggcctggcat gaacctatcc ataatttagg tgggatgccc ggtgcgtggc tggcagatac
    18181 gctgttcttt atttttggcg tgatggctta caccattccc gtcattattg tcggcggttg
    18241 ttggtttgcc tggcgtcatc agtccagtga cgaatacatt gattattttg ccgtttcgct
    18301 acgcatcatt ggcgttttgg cgctcatcct tacctcctgt ggtctggcgg caatcaacgc
    18361 tgacgatatc tggtattttg cctccggtgg cgtcattggc agcttactaa gcactacgct
    18421 acaaccactg ctacacagta gcgggggaac tattgcgctg ctctgcgttt gggctgcggg
    18481 cctgacgctg ttcaccggtt ggtcatgggt gaccattgct gaaaaactcg gcggctggat
    18541 tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc gatgatacct gggtcgatga
    18601 agatgaatat gaagatgacg aagagtatga agatgaaaac cacggcaaac agcatgaatc
    18661 gcgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt aaacggttgg cggaaaaatt
    18721 cattaatccg atggggcggc aaacagacgc tgcgttgttc tccggtaagc ggatggatga
    18781 tgacgaagag attatctaca ctgcacgcgg tgtggctgcc gacccggacg acgtcctatt
    18841 ttcgggcaat cgtgcaacgc agccagaata tgacgaatac gatccattat taaacggtgc
    18901 gccaattacc gaacctgtcg ctgtagcagc tgctgctacc acggcgacac aaagctgggc
    18961 tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc tctgttgatg ttccaccttc
    19021 gcaacctaca gtagcctggc agcctgtacc gggtccacaa acgggagagc cggttattgc
    19081 tcctgcaccg gaaggttacc cacagcagtc acaatatgcg cagcctgcag tgcaatataa
    19141 tgagccgctg caacaaccag tacagccgca gcagccgtat tatgcacctg cagctgaaca
    19201 acctgcgcaa caaccttact acgctcctgc ggcggaacaa cctgttcagc aaccgtatta
    19261 cgcccctgcg ccagaacaac cggtggcagg taacgcctgg caagccgaag agcagcaatc
    19321 cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcaac cagccgctca
    19381 ggagccgttg taccaacagc cgcaatccgt tgaacagcag cctgttgtgg agcctgaacc
    19441 cgttgtagaa gagacaaaac ccgcgcgtcc gccgctttac tattttgaag aagtggaaga
    19501 gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat caaccgattc cagaaccggt
    19561 taaagaacca gaaccgatca aatcttcgtt gaaagcgcct tctgttgcag cagtacctcc
    19621 agtagaagcc gctgccgctg tttctccgct ggcatctggc gtgaaaaaag cgacactggc
    19681 gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata gcggtggacc
    19741 gcgtccgcaa gtcaaagagg ggattggccc gcagttgcca cgaccgaaac gtatccgcgt
    19801 gccaactcgt cgtgaactgg cgtcttacgg tattaagctg ccctcacagc gtgcggcgga
    19861 agagaaagcc cgtgaagccc agcgcaatca gtatgattct ggcgatcagt ataacgatga
    19921 tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga cacagcagca
    19981 acgctatggc gaacagtatc aacatgatgt gcccgtaaac gcagaagatg cagatgctgc
    20041 ggcagaggct gaactggctc gtcagtttgc gcaaactcaa caacaacgtt attccggcga
    20101 acaaccggct ggggcgaatc cgttctcgct ggatgatttt gaattttcgc caatgaaagc
    20161 gttgctggat gatggtccac acgaaccgtt gtttacgcca attgttgaac ctgtacagca
    20221 gccgcaacaa ccggttgcac cgcagcagca gtatcagcag ccgcaacaac cagttccgcc
    20281 acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg cagccgcagt atcagcagcc
    20341 acaacagcag gttgcgccgc agccgcagta tcagcagcca caacaaccgg ttgcgccaca
    20401 gccgcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    20461 acaaccggtt gcaccgcagc agcaggatac cctgcttcat ccgctgttga tgcgtaatgg
    20521 cgacagccgt ccgttgcata aaccgacgac gccgctgcct tctctggatt tgctgacacc
    20581 gccgccgagc gaagtggagc cggtagatac ctttgcgctt gaacaaatgg ctcgcctggt
    20641 ggaagcgcgt ctggctgatt tccgtattaa agccgatgtc gtcaattact ctccggggcc
    20701 ggttatcact cgctttgaac ttaacctggc tcctggcgta aaagcggcgc gcatttctaa
    20761 cttgtcacgg gaccttgccc gttcactttc gacggtggcg gtgcgtgtcg ttgaagttat
    20821 tcctggcaaa ccctatgtag gtctggagtt accaaataaa aaacgacaaa ccgtttatct
    20881 gcgcgaagtt ttggataacg ccaaattccg cgataatccg tcgccattaa ccgtggtgct
    20941 gggtaaagat atcgccggtg agccggtggt tgccgatctg gcgaaaatgc cgcacttgtt
    21001 ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg aacgcgatga tcctgagcat
    21061 gctttataaa gcacagccag aagatgtgcg tttcatcatg atcgacccga aaatgctgga
    21121 gctttcggtt tatgaaggca ttccgcatct gttaacagaa gtcgttactg atatgaaaga
    21181 tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag cgtcggtata aactgatgtc
    21241 tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa attgctgaag ccgatcgcat
    21301 gatgcgtccg attccagacc cgtactggaa gccgggtgac agtatggatg cccagcatcc
    21361 ggtgctgaaa aaagaaccat acattgtggt gttggttgac gaatttgccg acctgatgat
    21421 gacggtaggt aaaaaagtgg aagagctgat agcacgtctg gcgcaaaaag cccgtgccgc
    21481 gggtatccac ctcgtactgg caactcagcg tccatcggtt gatgttatta ctggtctgat
    21541 taaagcgaat attccgaccc gtatcgcctt taccgtatcc agtaagattg actcacgtac
    21601 cattcttgat caggctggcg cggaatcact gctggggatg ggggatatgc tctactctgg
    21661 gccgaactcc acgttgccgg tacgtgtcca tggtgctttt gttcgcgatc aggaagttca
    21721 tgccgtggtg caggactgga aagcgcgtgg tcgcccacag tatgttgatg gcatcacttc
    21781 cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc gctgaagaac tggatccgtt
    21841 gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa gcgtcaattt ctggcgtaca
    21901 gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt atcgaacaga tggaagcgca
    21961 ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa gtgctggccc caccgccgtt
    22021 tgactaatta atgcattgcc ggataaggcg cggtagcgtc gcatccggca ctctatcaac
    22081 tgaaaattca gtattttctt ctttcctcaa gctgattatt agcctggaat agagagtaga
    22141 gggaactccc gatcgggagt gacgtaattt gaggaataat gatgaaaaaa attgccatca
    22201 cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg ggctgatgcc gcaagcgatc
    22261 tgaaaagccg cctggataaa gtcagcagct tccacgccag cttcacacaa aaagtgactg
    22321 acggtagcgg cgcggcggtg caggaaggtc agggcgatct gtgggtgaaa cgtccaaact
    22381 tattcaactg gcatatgaca caacctgatg aaagcattct ggtttctgac ggtaaaacac
    22441 tgtggttcta taacccgttc gttgagcaag ctacggcaac ttggctgaaa gatgccaccg
    22501 gtaatacgcc gtttatgctg attgcccgca accagtccag cgactggcag cagtacaata
    22561 tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc cagcaatggc aatctgaagc
    22621 agttcaccat taacgtggga cgtgatggca caatccatca gtttagcgcg gtggagcagg
    22681 acgatcagcg cagcagttat caactgaaat cccagcaaaa tggggctgtg gatgcagcga
    22741 aatttacctt caccccgccg caaggcgtca cggtagatga tcaacgtaag tagaggcacc
    22801 tgagtgagca atctgtcgct cgatttttcg gataatactt ttcaacctct ggccgcgcgt
    22861 atgcggccag aaaatttagc acagtatatc ggccagcaac atttgctggc tgcggggaag
    22921 ccgttgccgc gcgctatcga agccgggcat ttacattcta tgatcctctg ggggccaccg
    22981 ggtaccggca aaacaaccct cgctgaagtg attgcccgct atgcgaacgc tgatgtggaa
    23041 cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg aggcgatcga gcgcgcccgg
    23101 caaaaccgca atgcaggtcg ccgcactatt ctttttgttg acgaagttca ccgtttcaac
    23161 aaaagccagc aggatgcatt tctgccacat attgaagacg gcaccatcac ttttattggc
    23221 gcaaccactg aaaacccgtc gtttgagctt aattcggcac tgctttcccg tgcccgtgtc
    23281 tatctgttga aatccctgag tacagaggat attgagcaag tactaactca ggcgatggaa
    23341 gacaaaacgc gtggctatgg tggtcaggat attgttctgc cagatgaaac acgacgcgcc
    23401 attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa atacgctgga aatgatggcg
    23461 gatatggccg aagtcaatga tagcggtaag cgggtcctga agcctgaatt attgaccgaa
    23521 atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg atcgctttta cgatctgatt
    23581 tccgcactgc ataagtcggt acgtggtagc gcacccgatg cggcgctgta ctggtatgcg
    23641 cgaattatta ccgccggtgg cgatccgtta tatgtcgcgc gtcgctgtct ggcgatagcg
    23701 tctgaagatg tgggtaatgc tgatccacga gcgatgcagg tggcaattgc ggcctgggat
    23761 tgctttactc gcgttggccc ggcggaaggt gaacgcgcca ttgctcaggc gattgtttac
    23821 ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt ttaaagccgc gctggcggat
    23881 gctcgcgaac gtccggatta tgacgtgccg gttcatttgc gtaatgcgcc gacgaaatta
    23941 atgaaggaaa tgggctacgg gcaggaatat cgttacgctc atgatgaagc aaacgcttat
    24001 gctgccggtg aggtttactt cccgccggaa atagcacaaa cacgctatta tttcccgaca
    24061 aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct ggctggctga acaggatcaa
    24121 aatagcccca taaaacgcta ccgttaatgt tatcgttgcg gtaatgttgt tactgtatcc
    24181 ctgtggtcgc aggctgtggc cacatctccc atttaattcg ataagcacag gataagcatg
    24241 ctcgatccca atctgctgcg taatgagcca gacgcagtcg ctgaaaaact ggcacgccgg
    24301 ggctttaagc tggatgtaga taagctgggc gctcttgaag agcgtcgtaa agtattgcag
    24361 gtcaaaacgg aaaacctgca agcggagcgt aactcccgat cgaaatccat tggccaggcg
    24421 aaagcgcgcg gggaagatat cgagccttta cgtctggaag tgaacaaact gggcgaagag
    24481 ctggatgcag caaaagccga gctggatgct ttacaggctg aaattcgcga tatcgcgcta
    24541 accatcccta acttgcctgc agatgaagtg ccggtaggta aagacgaaaa tgacaacgtt
    24601 gaagtcagcc gctggggcac cccgcgtgag tttgactttg aagttcgtga ccacgtgacg
    24661 ctgggtgaaa tgcactctgg cctcgacttt gcagccgcag ttaagctgac aggttcgcgc
    24721 tttgtggtga tgaaagggca gattgctcgc atgcaccgcg cactgtcgca gtttatgctg
    24781 gatctgcata ccgaacagca tggctacagt gagaactatg ttccgtacct ggttaaccag
    24841 gacacgctgt acggtacggg tcagctgccg aaatttgctg gcgatctgtt ccatactcgt
    24901 ccgctggaag aagaagcaga caccagtaac tatgcgctga tcccaacggc agaagttccg
    24961 ctgaccaact tggtacgcgg tgaaatcatt gatgaagatg atctgccaat taagatgacc
    25021 gcccacaccc catgcttccg ttctgaagcc ggttcatatg gtcgtgacac ccgtggtctg
    25081 atccgtatgc accagttcga caaagttgaa atggtgcaga tcgtgcgccc ggaagactca
    25141 atggcggcgc tggaagagat gactggtcat gcagaaaaag tcctgcaact gctgggccta
    25201 ccgtaccgta aaatcatcct ttgtaccggc gacatgggct ttggcgcttg caaaacttac
    25261 gacctggaag tatggatccc ggcacagaac acctaccgcg agatctcttc ctgctccaac
    25321 gtttgggatt tccaggcgcg tcgtatgcag gctcgttgcc gcagcaagtc ggacaagaaa
    25381 acccgtctgg ttcataccct gaacggttct ggtctggctg ttggtcgtac gctggttgca
    25441 gtaatggaaa actatcagca ggctgatggt cgtattgaag taccagaagt tctgcgtccg
    25501 tatatgaacg gactggaata tattggctaa tacccaattt ttctgaatct aaaaagcgcc
    25561 tgcgggcgct ttttttgtct ccctttgata ccgaacaata attactcctc acttacacgt
    25621 aatactactt tcgagtgaaa atctacctat ctctttgatt ttcaaattat tcgatgtata
    25681 caagcctata tagcgagctg ctatagaaat aattacacaa tacggtttgt tactggaatc
    25741 aatcgtgagc aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc
    25801 tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc
    25861 cagcagcgca ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc
    25921 aacaaaatca gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg
    25981 cccgctacgt atgcacgtcg tggacggtga aatcaaatat gtcgaaacgg acaataccgg
    26041 cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgt ctgcgtggac gttccatgcg
    26101 tcgccgtgtc tataatccgg accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg
    26161 tgaaggcaaa tttgagcgca ttagctggga agaagcctac gacatcatcg cgaccaatat
    26221 gcagcgcctg atcaaagagt acggcaacga atccatctat ctgaactatg gcaccggtac
    26281 gctgggcggc accatgaccc gctcctggcc cccgggaaat accctggtcg cgcgcctgat
    26341 gaactgctgc ggcggttatc tgaaccatta cggtgactac tcttccgcgc aaattgcgga
    26401 aggtttgaac tatacctacg gcggctgggc agatggtaac agcccgtcgg atatcgaaaa
    26461 cagtaaactg gtagtgctgt ttggtaataa ccctggcgaa acgcgaatga gtggcggtgg
    26521 ggtgacttac tatcttgaac aggcacgcca gaaatctaat gcccgcatga tcatcatcga
    26581 tccgcgctat accgacaccg gtgccgggcg cgaagatgag tggatcccta ttcgtccggg
    26641 aacagatgcc gcactggtta acggtctggc gtacgtcatg atcactgaaa acctggtgga
    26701 tcaggcattc ctcgataaat attgcgttgg ctacgatgag aaaaccctgc cagccagtgc
    26761 gccgaaaaat ggccactata aagcttatat tctgggtgaa gggccagatg gcgtggctaa
    26821 aacaccggaa tgggcctcac aaatcaccgg tgttccggca gacaaaatca tcaaactggc
    26881 tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag ggatggggcc cgcagcgtca
    26941 cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga ccggtaacgt
    27001 tggtattaac ggaggcaaca gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg
    27061 tatgccgacc ttggaaaacc cgatccagac cagcatttcg atgtttatgt ggaccgatgc
    27121 cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt gtacgcggga aagataagct
    27181 ggatgtgccg atcaaaatga tctggaacta tgccggtaac tgcctgatta accagcattc
    27241 tgaaatcaac cgtacccatg aaatccttca ggatgataag aagtgcgagc tgattgtggt
    27301 tatcgactgc cacatgacct catcggcgaa atatgctgac atcctgctgc ctgactgcac
    27361 cgcttccgaa cagatggact ttgcgctgga tgcatcctgc gggaatatgt cttacgtgat
    27421 tttcaacgat caggtgatta aaccgcgctt tgaatgtaag accatctatg aaatgaccag
    27481 cgaactggca aaacgtcttg gcgttgagca acagtttact gaaggccgta cacaggaaga
    27541 gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt cctgaactgc caacgtttga
    27601 agagttccgc aagcagggga tctttaaaaa gcgcgaccca caagggcatc acgttgctta
    27661 taaagccttc cgtgaagatc cgcaggcaaa cccactgact acgccatcgg gcaaaattga
    27721 gatttattcg caggcgctgg ctgacattgc cgctacctgg gaattgccag aaggcgatgt
    27781 gatcgatcca ctgccgatct acacgccggg ctttgaaagt tatcaggatc cgctgaacaa
    27841 acagtatccg ctgcagctta cgggtttcca ctataaatct cgcgttcact caacttacgg
    27901 caacgttgat gtgctgaaag cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc
    27961 gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc tttaacgatc gtggtgaagt
    28021 tcatattgag gcgaaagtga cgccacgaat gatgccgggt gtggtcgcac tgggtgaagg
    28081 tgcctggtat gacccggatg caaaacgtgt cgataagggc ggttgtatta acgtactgac
    28141 cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca catacaaacc ttgttcaggt
    28201 tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg attttttatt gattccagcc
    28261 gttgcaccgg ttgcaaaacc tgcgagctgg cctgtaaaga ctacaaagat ttgacgccag
    28321 aagtcagctt ccgccgcatt tatgaatatg ctggcggcga ctggcaggaa gataacggtg
    28381 tctggcacca gaacgtgttt gcctactatc tgtcgatttc atgtaaccac tgcgaagatc
    28441 cggcttgtac taaagtctgc ccgagcggtg cgatgcataa acgcgaagat ggttttgttg
    28501 tggtcgatga agatgtatgc attggctgcc gctactgtca tatggcttgc ccgtatggcg
    28561 caccgcaata taacgaaacg aaaggccata tgaccaaatg cgatggttgt tatgaccgtg
    28621 ttgcggaggg taaaaagccg atctgtgttg aatcttgccc gctgcgggcg ctggatttcg
    28681 gtcctatcga cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc
    28741 gagctcactt taccaaaccg aatattgtga tcaaacccaa tgccaacagc cgcccgaccg
    28801 gggataccac tggctacctg gcaaacccga aggaggtgtg agatgggaag tggatggcat
    28861 gaatggccgc tgatgatctt cacggtcttc gggcaatgtg tagcgggtgg ttttatcgtt
    28921 ctggctttgg cgctgctcaa aggcgacctg cgagcagaag cccagcagcg tgttatcgcc
    28981 tgcatgtttg gcttatgggt gctgatgggc attggcttta tcgcctctat gctccatctt
    29041 ggttcaccga tgcgcgcctt taactcgctc aaccgggtag gggcttcagc actcagtaac
    29101 gaaatcgcca gcggttcgat cttttttgct gtaggcggca tcggctggtt gctggcaatg
    29161 ctgaaaaagc tgtcaccggc attgcgtacg ctatggctgg tggtgacgat ggttcttggc
    29221 gtcgtctttg tctggatgat ggtgcgtgtg tataacagca ttgataccgt tccgacctgg
    29281 tacagcatct ggacgccgat gggcttcttc ctgacgatgt tcatgggcgg cccgttactg
    29341 ggttacctgt tgctgagtct ggctggcgtc gatggctggg cgatgcgtct gctgccagcc
    29401 atttctgtac tggcactggt ggtgagtggt gtgatgtcag tgatgcaggg cacagagctg
    29461 gcaactattc atagttctgt gcagcaggcc gcagcgctgg tgccggacta tggtgcgctg
    29521 atgtcctggc ggatcgtgct tttggccgtt gccctgtgct tgtggattgc accacagcta
    29581 aaaggttatc agcctgcggt tccgctacta tccgtctcat tcattctgct tctggcaggg
    29641 gaattaatcg gtcgcggcgt attctatggt ttgcatatga ccgtggggat ggccgtcgca
    29701 agctaatcag taacaaccgg ggtttcggcc ccggttctct tttatttctg cttcgttaac
    29761 gtgtcataac tggtcatcaa attacgataa tcaggaatat ggttagagaa caacgtcgcc
    29821 agtccttcaa tatcattacg ccagtcacgg tgcagctcgc acgccacgcc gaaccatgtc
    29881 atcaattgcg cacctgcttg cgacatgcga tcccatgccg aatgccgggt aatttcatta
    29941 aaggtccctg acgcgtcagt cacgacaaag acgtcaaacc cttcttcaat ggctgaaagc
    30001 gccgggaatg ccacgcaaac ttcggttacc acaccggcaa taattaactg ttttttacct
    30061 gtcgctttga ctgcttttac aaaatcttcg ttatcccagg cgttaatatt tcccgggcga
    30121 gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg gaactaatgg accgttagga
    30181 ccggtttcaa aactggtggt aagaatagtt ggtaagttga aatacttagc caggtcaccc
    30241 agcgccagca cgttattttt aaacttatcg ggttcgatat cccgtacaag ggaaagtaaa
    30301 ccagcctggt gatcaacaag caaaacggca gcatcatttt tatcaagacg aacatacggt
    30361 ttggtcatcc tcttctcctt tcgagagtca gtggtacgtc tgaaatcggt cagacgcgat
    30421 gcattgctct gaaagcatag acgggaaata tgagtttgct gtgaccatga aatttttcga
    30481 ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa caatcagatg agattaatcg
    30541 tctcttcagc attagcgtta tgaatgagta atataaatcg ataataccgc cgctgcgata
    30601 tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg catgatgcgc atgaaatttg
    30661 aacttcctca cggttttaat tcatgtccac gtatacccgg cctgtcatgc ttttgctgtc
    30721 tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat acactcgtgc cgctttggct
    30781 cgcccaggaa cacatgtcca catggcaggt aggcgttgtc agctcatcct attttaccgg
    30841 caaccttgtc ggtacattgc tgacagggta tgtcattaag cgcattggct ttaaccgcag
    30901 ctattatctg gcctccttca tttttgccgc tggctgtgcc ggccttggcc tgatgattgg
    30961 attctggagc tggttggctt ggcgttttgt cgcgggcgtc ggctgtgcca tgatttgggt
    31021 ggttgttgag agcgcgctga tgtgcagtgg gacgttacgt aaccgtgggc gtttgcttgc
    31081 tgcgtatatg atggtttatt acgtgggaac gtttttaggc cagttactgg tcagcaaagt
    31141 ttcaaccgag ctgatgtccg tattgccgtg ggttacaggt ttgacgttgg cagggatctt
    31201 accgctgttg tttacgcgtg tgctgaatca gcaggctgaa aaccatgatt cgacgtcaat
    31261 tacgtcaatg ctaaaactcc gtcaggcgcg gcttggcgtg aatggctgca ttatctcagg
    31321 aatcgttctg ggatctctat atggcctgat gccgctgtac ctcaatcaca aaggggtgag
    31381 caatgccagc attggtttct ggatggcggt actggtcagt gcgggtatcc ttggacaatg
    31441 gccgattgga cgtctggcgg ataagtttgg tcgactgctg gtgttgcgtg ttcaggtctt
    31501 tgtcgtcatt ctcggcagta tcgcgatgct tagccaggcg gcgatggccc cagcgttatt
    31561 catcctcggt gccgctggct ttacgctata tccggtggcg atggcatggg cttgcgagaa
    31621 agttgaacat catcaactgg tggcgatgaa ccaggcctta ctgttgagtt atactgtggg
    31681 aagtctgctt ggcccgtcat ttaccgctat gctaatgcag aatttctccg ataatttatt
    31741 gtttatcatg atcgccagcg tatcgtttat ctatttgctg atgctgctgc gcaacgccgg
    31801 tcatacgccg aaacccgttg ctcacgtgta aatgaattca agcagagtgt gaacttactg
    31861 tttcacactc tgcttttttg tttcttctat ctgacttgct ttattccaaa ttttattcgt
    31921 ttaaaaataa aatgtgcagc aggttataat tttgcatttc gctatttccg cacttcttat
    31981 ttgccgcgca taatccctcg ttttacagat gcccctttaa ttttggcgaa ggatttgtct
    32041 atggctggga atgttcagga aaaacagttg cgatggtaca acattgcgct gatgtctttt
    32101 atcactgtct ggggttttgg caacgttgtt aataactatg ccaaccaggg gctggtggtt
    32161 gttttttcat gggtgtttat ctttgcactc tatttcacac cttatgcgct aattgttggt
    32221 cagttaggct cgaccttcaa agatgggaag ggcggggtca gtacctggat taaacacacg
    32281 atgggacccg gactggctta tctcgccgcg tggacctact gggtggtgca tattccctat
    32341 ctggcacaaa aaccccaggc aattctgatt gcgctcggtt gggcgatgaa aggcgacggt
    32401 tcgctaatca aagaatattc agtcgtagcg ttacaggggt taacgctggt gctgtttatc
    32461 ttctttatgt gggttgcttc acgcggtatg aaatcgctga aaatcgtcgg ttctgtggca
    32521 gggattgcga tgtttgttat gtcgctcctg tatgtggcga tggcggtaac cgcgcctgcg
    32581 attactgaag tgcatattgc gaccacaaac attacctggg aaacgttcat tcctcatatc
    32641 gactttacct acattaccac tatttcaatg ctggttttcg cggttggcgg agcagagaag
    32701 atttctcctt acgttaatca aacgcgcaac ccaggaaaag aatttccaaa agggatgcta
    32761 tgcctggcgg tgatggttgc ggtttgtgcc attctgggct cactggcgat ggggatgatg
    32821 tttgactcgc gtaatatccc ggatgactta atgactaacg gtcaatatta cgcctttcag
    32881 aagctgggcg agtattacaa catgggtaat actttaatgg tgatttacgc cattgcgaat
    32941 accctgggac aagtagctgc gctggtattc tcgattgatg ccccgcttaa agtgctatta
    33001 ggtgatgctg acagcaaata tattccagcc agtttatgtc gtaccaacgc ttctggtacg
    33061 cccgttaatg gctattttct gaccctggtg ctggtggcaa ttctgattat gctgccgact
    33121 ctcggcatcg gtgatatgaa aaatctctat aaatggctgt tgaatcttaa ctcggtagtg
    33181 atgccgctgc gttatttatg ggtatttgtt gcatttattg cagtcgttcg cctggcgcag
    33241 aaatataaac cagaatatgt ctttattcgt aataagcctc tggcaatgac cgtcgggatt
    33301 tggtgttttg cctttaccgc ctttgcctgc ttgacgggga tcttcccgaa aatggaagcc
    33361 ttcactgcag agtggacctt ccagttggcg ctgaatgttg caacgccgtt tgtgctggta
    33421 ggattaggac tgatattccc gctgctggcg cgtaaagcga atagtaaata attattgtgg
    33481 tcggtgacgc tgactctggc agatctgacc cgtttttaca taatcgagaa atgcgcgcaa
    33541 gccgcaggac atatactggc ggtttggata atagatctgg aagcctgaac gctctgtgct
    33601 ccagtcttcc agtacacatt ctaaacgacc agtatcaagg tactccttga tctgttcata
    33661 gagcagatac ccgataccag cacccatcaa aacggcctcc agttcagcat caacatcatc
    33721 cagaatgata tttcctgcga cagcaatttc cagctccttt gcaaattgcc agtggaaagg
    33781 tttgccgctg ggataacgaa aaacaacaca ttgatgatta agcagatcat gaggatggcg
    33841 gggtttgcca tatcttgcaa agtactctgg cgttgctgct acacataatt tcactggtgg
    33901 gccgatcgcc acgctgatca tatctttttc gacgatgcca ctaagtcgaa cgccagcgtc
    33961 aaagccctgt tggacgatat cgacaagaga atcgtcagtt gtgagctcga ctttaatatc
    34021 aggatattca cgtgtgaaac caaccagtaa aggcattaaa aatatacgtg cagctacccg
    34081 ggcggcattg attttgagcg ttcctgtcgg tgtcagacga aaatcattca tctcatctaa
    34141 catgatctga atttcgtcga aagctgggcg caatcgttca tagagattag aacccgcttc
    34201 cgttaacgat acgcttcggg ttgtccgatt gaataagcga attttaagac gttgttccag
    34261 tgttttaata ctatggctaa tggcggacga ggataagcct aattcatcgc ccgctgcacg
    34321 aaagctttga ttacgggcca cggcaaagaa agtggcaaag tcagacatat tcatccgcat
    34381 tagtgaaaat ccttcattaa ctcatcctga atcatcacgt ttacaggatt atgccgcctg
    34441 cgtaaagtgc gctccagaac ttaacgtgga ggtaaaatta tgcagtctga acgtatttat
    34501 ttggtatggg cccatcctcg tcatgattca ttgaccgcac atattgctga tgcgatccat
    34561 cagcgggcaa tggagcggaa aatacaggtg acggaactcg atttatatcg gcgtaatttc
    34621 aacccggtga tgacgtcgga agatgaacca gactggaaga atatggataa acgttattct
    34681 ccagaggttc atcagcttta ttcagagctg cttgaacatg acacgttagt ggtggttttt
    34741 cctctctggt ggtacagctt cccagcaatg ctaaaaggat atattgacag agtatggaat
    34801 aatgggctgg cttatggaga tgggcacaaa ttaccattca ataaagttcg ttgggtggcg
    34861 ctggttggag gagacaaaga atcatttgtc cagatgggct gggaaaaaaa tataagcgat
    34921 tatttgaaaa atatgtgcag ttatcttggt attgaagatg ccgatgtcac tttcttgtgt
    34981 aatacagtgg tattcgatgg gcaagaactt catgcgagct attatcagtc gttattatct
    35041 caggtacggg atatggtaga tgcactataa aatgtgttaa aaacgctgta gcagaatgaa
    35101 gcgcggaata aaaaagcggc aactcaataa agttgccgct ttacggggaa attagaacat
    35161 taccttatga ccgtactgct caagaatgcc tttcacgcgt tccatggtct ctttcttagg
    35221 tggtttaaca ccgtcgagtt tgtactcttc acccattgcc acccatttgt gtttgcccag
    35281 ctcgtggtag gggaggagtt cgattttctc aacgttgccc atatcacggg taaattcacc
    35341 gaggcgatgc gctgaatcgt catcgtcaga ccagcctggg acaacaacgt agcggatcca
    35401 caccttcaca tttttgttcg ccagatattt ggcgaactcc agcgtgcggt ggttggaaac
    35461 tccaaccaga ttttggtgga tctcgtcgtt catctgtttg agatcgagca ttaccaggtc
    35521 ggttacttcc agcagttcat caatcaccgg atcgtaacga cgaacaaaac cgttggtgtc
    35581 cagacaggta tgaatgcctt cttttttgca ggcgcggaac cagtcacgaa caaactcagc
    35641 ttgtaggatt gcctcaccgc cggatgcggt aacgccgccg ctggaagcgt tcataaagtg
    35701 gcgataggtc accacctctt tcattaaatc ttcaacggta acttctttac cgccatgcgt
    35761 atcccaggtg tcgcggttat gacaatacag gcagcgcatc aggcagccct ggaaaaaggt
    35821 gataaagcga atacctgggc cgtctacggt tccacaggat tcaaaggagt gaatgcgacc
    35881 aataactgac attgcggtgt ttctccagat gtggcccatc tgaggccgtg ttggtgcgca
    35941 gctcgaaggc tacgtcgagt ctgttttggc agtcacctta aagtatagat agctgacaaa
    36001 aaaggctctc gtgctaaaaa aggccccact ttcgtggagc ctttattgta cgctttttac
    36061 tgtacgattt cagtcaaatc taattacata gattgagtga aggtacgagt aataacgtcc
    36121 tgctgctgtt ctttagtcag cgagttgaaa cgtactgcgt agccagatac acggatggtc
    36181 agctgcggat atttttccgg gttttccatc gcgtcgagca gcatttcacg gttcatcacg
    36241 ttaacgttca ggtgctgacc accttcgatg gatgcttcgt ggtggaagta accatccatc
    36301 agaccagcca ggttggtctt acgaacttcg tcgtctttac ccagtgcgtt cggaacgata
    36361 gagaaggtgt aggagatacc atctttagcg taagcaaacg gcagtttagc aacggaagtc
    36421 agagacgcta cagcaccttt ctggtcacga ccgtgcatcg ggttagcacc cggtccgaac
    36481 ggcgcgccag cacgacgacc gtctggggtg ttaccagttt tcttaccata cacaacgtta
    36541 gaagtgatgg tcagaacaga ctgagtcggg atagcgtcac ggtaggtgtg cagtttctga
    36601 attttcttca tgaaacgttc taccaggtca acagccaggt catctacacg cggatcattg
    36661 ttaccaaact gcgggtattc gccttcgatt tcgaagtcga tagccagacc gtcttcgtca
    36721 cgaatcggtt taactttcgc atatttgatt gcagacaggg agtcagcagc aacggacagg
    36781 ccagcgatac cacacgccat ggtgcggata acgtcacggt cgtgcagcgc catcagagag
    36841 gcttcgtagc tgtacttgtc gtgcatgtag tggatgatgt tcagtgcagt gatgtactgt
    36901 ttagccagcc agtccatgaa gtgatccatg cgctccatca cttcatcata gttcaggaca
    36961 tcgcctttga tcggttcaga cttcggacca acctgcattt tcagtttttc gtcaacgccg
    37021 ccgttgattg cgtacagcat ggttttcgcc aggtttgcac gcgcaccgaa gaactgcatt
    37081 tgtttaccaa cgatcatcgg gcttacgcag caagcaatag cgtagtcatc gttgttgaag
    37141 tccggacgca tcaggtcatc gttctcatac tgcagagaag aggtgtcgat ggacacttta
    37201 gcggcgaatt tcttgaagtt cagcggcagt ttttcagacc acagaatggt catgttcggt
    37261 tccggagacg gacccatggt gtacagggtg ttcaggaaac ggaagctgtt tttggtaacc
    37321 agggtacgac cgtcgaggcc cataccaccg atagattcgg ttgcccagat cgggtcgcca
    37381 gagaacagtt catcgtattc cggagtacgc aggaagcgaa ccatacgcag tttcatgacc
    37441 aggtggtcaa ccatttcctg cgcttcttgt tcggtgatct tgccagcttt caggtcacgt
    37501 tcgatgtaca catccaggaa ggtggaggta cgaccgaagg acattgcagc accgttctga
    37561 gacttaacag cagccaggta gccgaagtaa gtccactgga tagcttcctg agcgttggta
    37621 gccggaccag agatgtcgta gccgtatttc gtagccattt ctttcatctg acccagagcg
    37681 cggtgctgtt cagcgatttc ttcgcgcaga cggatagtct gttccaggtt tacgccgttt
    37741 tccagatcag cctgcagaga agtgaactgt gccagtttgt ctttcatcag gtagtcgata
    37801 ccgtacagcg caacgcgacg gtagtcaccg atgatacggc cacggccata tgcatctggc
    37861 agaccggtca gaacaccaga tttacggcaa cgcaggatgt ccggagtgta aacgtcgaac
    37921 acgccctggt tgtgagtttt acggtattca gtgaagattt ttttgatcat cggatccagt
    37981 tcgcggttgt acgctttgca ggaaccttcg atcattttga taccaccgaa cgggataaga
    38041 gcacgtttca gcggagcttc agtctgcaga ccaacgattt tctcaagctg cttgttgatg
    38101 tagccagcgt cgtgagaggt gatggtggaa gcaacagcgg tgtcaaagtc aactggcgcg
    38161 tgagtgcggt tttccagttt aacgccttcc attactttgt cccacagggt ggtggtcgct
    38221 tcagtagcgc cagccaggaa ggactcgtca ccctcgtacg gagtgtagtt tttctgaatg
    38281 aagtcacgga cgtttacttc attctgccag tcacctttgg taaaaccttc ccaggctgtg
    38341 gctaactttt cattaagctc ggacatgtaa cacctacctt cttaagtgga ttttttattt
    38401 actgcgtact tcgacaacca ttaatggtgg tcgttttcac gcaggtaaat gacccagtat
    38461 gtcaacccaa ccaacaaacc accaccgata atgttgccga tcgtaaccgg aatcaggtta
    38521 tcagtgatga aattcatcac ggtcaggtga gaaaaatttt ccggtgcaga accgactgcg
    38581 gtccaaaatt ccggggatgc gaagtcgcgg attacaatac ccatcgggat cataaacatg
    38641 tttgcgatac tgtgctcaaa accgctggca acaaacatcg cgaccggcag caccataatg
    38701 aacgctttgt ccatcaggct gcggccagaa taactcatcc atactgccag acataccatc
    38761 aggtttgcca ggataccaag acagacggcc tcaataaaag tatggtgcac tttgtggtcg
    38821 gcggtttgta ggacgtttag tccccattga ccatttgcgg tcatatactc gccggaaagc
    38881 cacattaaaa gtacaaacag cagtgcgccg accaggttgc caaaatagac atttagccag
    38941 tttttcgcca actgacccca ggtgatgcgc ccactcgcct tagcaacaac aatcaacacg
    39001 gtggaagtaa agagatcggc tccgcagaca acacaaagaa tcagccccag agagaagcaa
    39061 atgccgccaa ccagttttgc catgccgaag ggcattgtgc ctgtgccagt ggttgctgtg
    39121 atatagaaga cgaatgcgat tgagatgaaa acaccggcgg taatcgccag atagaaagtc
    39181 ttaagcggat gtttcgttgc tttatagaca cccgcctctt cggccacttt ggccattgca
    39241 gcaggaagta aaagatcaaa agggttgtca gctttcacac taactctctc tttattaagt
    39301 cggcgacgag atactaacaa agcattatag atgagaaatt gatatagatc atatctcgcc
    39361 tggcttatag gcccgtaact cgcatggttt ttatgca
//
LOCUS       DAZFYC010000036        37848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFYC010000036 DAZFYC010000000
VERSION     DAZFYC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.036"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..378
                     /locus_tag="ACHKW5_002031"
                     /pseudo
     CDS             241..378
                     /locus_tag="ACHKW5_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(486..1721)
                     /gene="aslB"
                     /locus_tag="ACHKW5_002032"
     CDS             complement(486..1721)
                     /gene="aslB"
                     /locus_tag="ACHKW5_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7511436.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1868..1944)
                     /locus_tag="ACHKW5_002033"
     tRNA            complement(1868..1944)
                     /locus_tag="ACHKW5_002033"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:Pro,seq:tgg)
     gene            complement(1987..2073)
                     /locus_tag="ACHKW5_002034"
     tRNA            complement(1987..2073)
                     /locus_tag="ACHKW5_002034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2037..2039),aa:Leu,seq:cag)
     gene            complement(2094..2169)
                     /locus_tag="ACHKW5_002035"
     tRNA            complement(2094..2169)
                     /locus_tag="ACHKW5_002035"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2134..2136),aa:His,seq:gtg)
     gene            complement(2228..2304)
                     /locus_tag="ACHKW5_002036"
     tRNA            complement(2228..2304)
                     /locus_tag="ACHKW5_002036"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2268..2270),aa:Arg,seq:ccg)
     gene            complement(2407..3792)
                     /gene="yifK"
                     /locus_tag="ACHKW5_002037"
     CDS             complement(2407..3792)
                     /gene="yifK"
                     /locus_tag="ACHKW5_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7511437.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3983..4723)
                     /gene="wecG"
                     /locus_tag="ACHKW5_002038"
     CDS             complement(3983..4723)
                     /gene="wecG"
                     /locus_tag="ACHKW5_002038"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO7511438.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4726..6078)
                     /gene="wzyE"
                     /locus_tag="ACHKW5_002039"
     CDS             complement(4726..6078)
                     /gene="wzyE"
                     /locus_tag="ACHKW5_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO7511439.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6075..7154)
                     /gene="rffT"
                     /locus_tag="ACHKW5_002040"
                     /gene_synonym="wecF"
     CDS             complement(6075..7154)
                     /gene="rffT"
                     /locus_tag="ACHKW5_002040"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO7511440.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFAARGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7151..8401)
                     /gene="wzxE"
                     /locus_tag="ACHKW5_002041"
     CDS             complement(7151..8401)
                     /gene="wzxE"
                     /locus_tag="ACHKW5_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO7511441.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8403..9533)
                     /gene="rffA"
                     /locus_tag="ACHKW5_002042"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8403..9533)
                     /gene="rffA"
                     /locus_tag="ACHKW5_002042"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO7511442.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9538..10212)
                     /gene="rffC"
                     /locus_tag="ACHKW5_002043"
                     /gene_synonym="wecD"
     CDS             complement(9538..10212)
                     /gene="rffC"
                     /locus_tag="ACHKW5_002043"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO7511443.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10190..11071)
                     /gene="rfbA"
                     /locus_tag="ACHKW5_002044"
     CDS             complement(10190..11071)
                     /gene="rfbA"
                     /locus_tag="ACHKW5_002044"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO7511444.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11090..12157)
                     /gene="rffG"
                     /locus_tag="ACHKW5_002045"
     CDS             complement(11090..12157)
                     /gene="rffG"
                     /locus_tag="ACHKW5_002045"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7511445.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12157..13419)
                     /gene="wecC"
                     /locus_tag="ACHKW5_002046"
     CDS             complement(12157..13419)
                     /gene="wecC"
                     /locus_tag="ACHKW5_002046"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO7511446.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13416..14546)
                     /gene="wecB"
                     /locus_tag="ACHKW5_002047"
     CDS             complement(13416..14546)
                     /gene="wecB"
                     /locus_tag="ACHKW5_002047"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO7511447.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14602..15648)
                     /gene="wzzE"
                     /locus_tag="ACHKW5_002048"
     CDS             complement(14602..15648)
                     /gene="wzzE"
                     /locus_tag="ACHKW5_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO7511448.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15660..16763)
                     /gene="wecA"
                     /locus_tag="ACHKW5_002049"
     CDS             complement(15660..16763)
                     /gene="wecA"
                     /locus_tag="ACHKW5_002049"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO7511449.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17003..18262)
                     /gene="rho"
                     /locus_tag="ACHKW5_002050"
     CDS             complement(17003..18262)
                     /gene="rho"
                     /locus_tag="ACHKW5_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO7511450.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18347..18448)
                     /gene="rhoL"
                     /locus_tag="ACHKW5_002051"
     CDS             complement(18347..18448)
                     /gene="rhoL"
                     /locus_tag="ACHKW5_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO7511451.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18589..18918)
                     /gene="trxA"
                     /locus_tag="ACHKW5_002052"
     CDS             complement(18589..18918)
                     /gene="trxA"
                     /locus_tag="ACHKW5_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO7511452.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19049..20314
                     /gene="rhlB"
                     /locus_tag="ACHKW5_002053"
     CDS             19049..20314
                     /gene="rhlB"
                     /locus_tag="ACHKW5_002053"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO7511453.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20448..21932
                     /gene="gppA"
                     /locus_tag="ACHKW5_002054"
     CDS             20448..21932
                     /gene="gppA"
                     /locus_tag="ACHKW5_002054"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO7511454.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21979..24000)
                     /gene="rep"
                     /locus_tag="ACHKW5_002055"
     CDS             complement(21979..24000)
                     /gene="rep"
                     /locus_tag="ACHKW5_002055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO7511455.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALTVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24217..24666
                     /locus_tag="ACHKW5_002056"
     CDS             24217..24666
                     /locus_tag="ACHKW5_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO7511456.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24865..25146
                     /gene="ppiC"
                     /locus_tag="ACHKW5_002057"
     CDS             24865..25146
                     /gene="ppiC"
                     /locus_tag="ACHKW5_002057"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO7511457.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25233..26708)
                     /gene="ilvC"
                     /locus_tag="ACHKW5_002058"
     CDS             complement(25233..26708)
                     /gene="ilvC"
                     /locus_tag="ACHKW5_002058"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO7511458.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26858..27751
                     /gene="ilvY"
                     /locus_tag="ACHKW5_002059"
     CDS             26858..27751
                     /gene="ilvY"
                     /locus_tag="ACHKW5_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO7511459.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27803..29347)
                     /gene="ilvA"
                     /locus_tag="ACHKW5_002060"
     CDS             complement(27803..29347)
                     /gene="ilvA"
                     /locus_tag="ACHKW5_002060"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO7511460.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29350..31200)
                     /gene="ilvD"
                     /locus_tag="ACHKW5_002061"
     CDS             complement(29350..31200)
                     /gene="ilvD"
                     /locus_tag="ACHKW5_002061"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO7511461.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31265..32194)
                     /gene="ilvE"
                     /locus_tag="ACHKW5_002062"
     CDS             complement(31265..32194)
                     /gene="ilvE"
                     /locus_tag="ACHKW5_002062"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO7511462.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIDVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32214..32477)
                     /gene="ilvM"
                     /locus_tag="ACHKW5_002063"
     CDS             complement(32214..32477)
                     /gene="ilvM"
                     /locus_tag="ACHKW5_002063"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO7511463.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32474..34120)
                     /gene="ilvG"
                     /locus_tag="ACHKW5_002064"
     CDS             complement(32474..34120)
                     /gene="ilvG"
                     /locus_tag="ACHKW5_002064"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO7511464.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34123..34173)
                     /gene="ilvX"
                     /locus_tag="ACHKW5_002065"
     CDS             complement(34123..34173)
                     /gene="ilvX"
                     /locus_tag="ACHKW5_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO7511465.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34260..34358)
                     /gene="ilvL"
                     /locus_tag="ACHKW5_002066"
     CDS             complement(34260..34358)
                     /gene="ilvL"
                     /locus_tag="ACHKW5_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO7511466.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34712..36232
                     /gene="yifB"
                     /locus_tag="ACHKW5_002067"
     CDS             34712..36232
                     /gene="yifB"
                     /locus_tag="ACHKW5_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO7511467.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36257..36595)
                     /gene="maoP"
                     /locus_tag="ACHKW5_002068"
     CDS             complement(36257..36595)
                     /gene="maoP"
                     /locus_tag="ACHKW5_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO7511468.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36714..37553
                     /gene="hdfR"
                     /locus_tag="ACHKW5_002069"
     CDS             36714..37553
                     /gene="hdfR"
                     /locus_tag="ACHKW5_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO7511469.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37649..37724)
                     /locus_tag="ACHKW5_002070"
     tRNA            complement(37649..37724)
                     /locus_tag="ACHKW5_002070"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37689..37691),aa:Trp,seq:cca)
     gene            complement(37733..37809)
                     /locus_tag="ACHKW5_002071"
     tRNA            complement(37733..37809)
                     /locus_tag="ACHKW5_002071"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37773..37775),aa:Asp,seq:gtc)
BASE COUNT         9266 a        10311 c         9511 g         8760 t
ORIGIN      
        1 gccgttaaaa ctaaaaaagc atcaataatc aacgtgatat aataaacctg ccttacatat
       61 caactgcgcc agaggtagga ttgaaaacgc tctcctgatt ttccaattca ttttctggat
      121 aaataaataa tttattttta tcactattat ttatgtaatc atcctgtcag ggagagggat
      181 ctcaattatc aatgcttaat tacgtcatca ttttgatgac atgaaaccgg agcattttgc
      241 atggaatttt cgttttcacc caaacttctt gttgttgctg tcgccgccgc tcttcctctc
      301 atggccagcg cagcagatac cccgtcaact gccaccgcac gcaaaggctt tgccggatac
      361 gatcacccaa accagtatcc acagaacgct gcaatttatt gaatttgcac gatcatgtag
      421 gccggataag gcgtttacgc cgcatccggc aatcaaccgc aggcggccac aaaatggccg
      481 cttttttact tattcaccac cagcaaatgt gcctgcataa tgtcactggc cgggcgaccg
      541 tgcgccagca aatcagccat tgctttaaga tatggcggta gatggcggaa ataacgctga
      601 tacccggcac acaaataatt cagccccggt ttgccgctgg catcgagcat gaagcggtgt
      661 ttcgggcagc ctccccagca cgcttttaac acgttacaac tgcgacactg cgccggtaat
      721 tgcttaaatt tatcttcacc aaacacctgc tgttgcgggg aatcgatcat ttctgcaatt
      781 gtttgctgat gcatattccc cagccgatat tgcggataaa catagtgatc gcaggcgtaa
      841 acgtcgccgt tgtgctcaac aatcaccgag cgcccacagg ttggctgatg atggcaaacc
      901 gcacccggcg caccgacaaa attggcaaac gcccattcga tattcatcac gaaaatcttg
      961 ccgacgtcgc gtttgatcca gtggtcgaat atcgccacca gaaattcacc aaattcatcg
     1021 gggcgcaccg accattccgt cagttccccc tgaatatcac caggcgcatg tagtttcagt
     1081 ccatcgctgg cagctgtttc atcagccagg cgctcgacca ccggaataaa ctggatgaat
     1141 tcgactcccg catcgcacaa aaaatcataa acctgcaacg gttgctgcgc gctggtgcgg
     1201 ttgacgcaga ccagcacgtt atagtcgaca tgatgttttt gcagcagcgt cagggcacgc
     1261 atcaccagct tatgcgttgg tctgccacct ttggtcacgc gatattgatt gtggatctca
     1321 gccggaccgt ccagcgataa cccaacaaga aaatgatttt ctgccagaaa tgcacaccat
     1381 ttatcatcga gcagcacgcc gttagtctgg aagctgttac ttatcttcct gccagcaccg
     1441 tatttcgcct gtaactttac ggcacggcgg taaaaatcca gcccgagtaa cgttggttcg
     1501 ccgccctgcc aggtaaaagc cacttcgttt tgcggttcgc tggcagcgat atagtgacgg
     1561 acatacgctt ccagcgtgtc atcgtccata tgcgtgactg gcttttcgcg gtaaagggat
     1621 tgtttttcga gataaaaaca gtagtcacag ttcagattac aatcggaacc actcggtttc
     1681 gccatcacat gaaaagcacg cgttggaacc tgttgcagca tggtcactcc ttgcctgaag
     1741 tcatccttca atacgttaag ggcgaaatca taccgtgggg caatgagtta ttacgggcgt
     1801 ttattgcgaa gggagggtga cgaaatgcac agaaaacaaa aaaccccgcc gaagcggggt
     1861 tcaaaattgg tcggcgagag aggattcgaa cctccgaccc actggtccca aaccagttgc
     1921 gctaccaagc tgcgctactc gccgaaatac tgctttttga atttttagtt caattcttta
     1981 aagtcgtggt gcgagggggg ggacttgaac ccccacgtcc gtaaggacac taacacctga
     2041 agctagcgcg tctaccaatt ccgccacctt cgcattgtca caacttctaa taatggggtg
     2101 gctaatggga ttcgaaccca cgacaactgg aatcacaatc cagggctcta ccaactgagc
     2161 tatagccacc actacaaatc ttgttacgcg gtattactac caccgcagct caagcgccgg
     2221 gactaaatgg cgcgcccgac aggattcgaa cctgagacct ctgcctccgg agggcagcgc
     2281 tctatccagc tgagctacgg gcgcttagcg ccgttgcggg ggtggataat acggacttcc
     2341 cacccctctg tctagtccct ttttaaataa attgcgcgtt tggttacggt ttgtgcgttt
     2401 tgctgattat tcctccagtt tatgcgcttt cccgtggcga ttaaggccaa aaactttata
     2461 aatcgccgtc accgccagca taaagatgat gccaacaaac agcgacatac gcgtatcttc
     2521 attaaagtac atgccgatca aaacgcaaat caggaatgcc attgttacgt agttggccca
     2581 cgggaacagg attgagcgga acggatggct ggcaatcgcc gctttatgtg cacgccgaaa
     2641 acgcagctgg cttatcaata tcacaaacca tggcaccatc cccggaagca cgctggcact
     2701 gtagacgtag acaaacacac gctgcggatt gggaatgatg tagttcaggc atgagccaat
     2761 tagcagaata gcaatagata ctgccacacc cgcaacgggt acgccgtgac gggaaacttt
     2821 cgccatcgcc gccggtaact gacggttttt cgccagtgcg tagagcatac gtccgcaact
     2881 gtacatgccg ctgttacagc cagagagcgc agccgtcagc accacaaagt tgataatgcc
     2941 cgccgctgcg gtaataccga ttttggcaaa agtcagtacg aacgggctgc cgttgctgcc
     3001 tatttcattc cacgggaaga tggtgacgat aacgaaaatc gcgcctacgt agaaaatcag
     3061 gatccgccac agcaccttgc ctacggcact gcgcagcgtc acctgcggat tcttcgcttc
     3121 accggcagta atgccaatca gctccacgcc ctggtaggac gccaccacaa tacacagcgc
     3181 ggtcaggaac cctttccagc cacccgcaaa gaaaccgcca tgctctgtga gattgctaaa
     3241 accaatcgac tgcccgccat tgccaaagcc aaagaaaatc acgcccagac caatgacaat
     3301 catcacgata atcgtggtga ctttgatcat cgcgaaccag aactcgattt cgccgtacaa
     3361 ccgcaccgcc gccagattcg ccaacgccac cagcgccact gcgatcaatg cgggtatcca
     3421 ctgcgccatc tccgggaacc agaactggac ataaacgcca atggcggtga tttctgagat
     3481 ccccaccgcc atccacataa accaataaga ccaggcggtg agataaccaa agaacgggct
     3541 catataacga tgcgcataaa cggcgaacga accggtaacc ggttcgagga acaacatttc
     3601 gcccattgaa cgcatgatga aaaagacgaa tagcccggcg atgatatagg ccaacaatac
     3661 ggatggcccg gcccatttca aggtactggc ggcccccata aacaggccga cgccaatggt
     3721 gccccccagg gcgatgagtt cgatatgtcg agcttccagc ccacgctgta gctctggttt
     3781 gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt
     3841 tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag gagaaatggc aaataaagca
     3901 ttaaaaattt gaatgctttg tgtaataaaa aagcagacag gcgacggagt gaccactccg
     3961 tcgctttaca aagagaggaa aatcagaggt tgccggtgta gtgccagcgt aaataacgca
     4021 gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag caggcggtag agccactcca
     4081 gtcccagcgt ttgccagatt ttcggtgcgc gttttacgtg accggtgaaa acatcgtaag
     4141 tcccgccaac gcccatatac agcgcatctg gatgcaccag acggcagtcg cgcatgaaga
     4201 tctcctgctt tggcgatccc atcgcaacgg tgacgatttg cgctccgctg gcatgaatgc
     4261 gttcaaacag cgcctgacgc tgctcgggtt taaaataacc atcctgactg ccaacgatat
     4321 tcacattcca ttggttgcgc agtttagctt cagtttgcgc cagcacttca ggtttaccgc
     4381 ccacaagaaa taccggcgtc ccttctttgc ctgcgcgcgc catcagctct tcccagagat
     4441 cggcaccggc aacgcgggaa acctgcgcct gcgggtactt tttacgtact gaacgtacaa
     4501 cgctgatgcc atccgcatat ttaaattcgg cagcgttaat taactccctg acctcggcgt
     4561 tatcttcaat ggtcagcatt ttttcggcat taatggcaac cagcgttccc tgcttaagct
     4621 gcccgtcagc aaacagataa tcgagggcgt gctgcatatc acgccaacca atcaactgta
     4681 agccacgcag cgtataggtc ggtgccgtgg tgttgttatt cattgttatc cttcaacctg
     4741 cgtccggagc gatgattttg tacgtttatg aatgagtccg gcgctttcaa aaagccagta
     4801 caacagtttt gcgatcatca gacatgcgcc gaagaccacg ataaaaaaga ctacgcgtga
     4861 gacaaacgaa tccagccctt cacgtgccag cacgatcata ttgaagatcg ccccaaagca
     4921 gaaactgtgc aatatcgcag ccttatagcg attagtctcg cggttgccca gctcatacag
     4981 ccagtcgaac catttgatga tcagtccaac cacaatcgcc ccgagtggga tgaacaacgc
     5041 gccgcccatc accaccagtg agcctataag cgtaggcgag atcgccagtc cggagtggtt
     5101 attcagcact tcccaggtaa agtagttggc tgagttcagc accatactcg ggcgacccgg
     5161 ccacagccag gaagggataa agacatagaa atcgcggaca attggagcca ggccctggaa
     5221 gtcgatgttg tcgtagttct gcaacagcaa cgccagattc tcccatggcg agaaggtgtc
     5281 gcgagtgaga tagagaaacg tatagaacgc ttcatcgccg ctcacattca ttccatagcg
     5341 ttttagcgcc agccagaaca tgcccacaat ccccagcacg cccgccgccg ccagcatcca
     5401 caacgaaatc cagccgcgaa taatgccaat aaacaggaag atagcgaatg cgatgatgat
     5461 attggcgcga gtgccgccga caatcatata agtcagcaag ccaaaggcga ccgtgctgac
     5521 gaggaaaaac agccacgctt tgctatcctg gcgcagaaag tagaccacca gcatcgccgg
     5581 gatgaaaaag taaaagaagc gttttaacgc cacgccggag acttcactgg agaagatctg
     5641 actgtaggag ttaagccgga acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct
     5701 taccagcgcg atgcccatca ggatcaccca cgtaagattg gtctccacgc ggttcatggt
     5761 aaacagcgga cggcgcggta catcagcaac gcgtttgcgt aggcgggttt tgtaggtgac
     5821 atagtaaacc gcgtagaagc agcccgcaga aagcaacgcc tgcaacaaga tttctggcgg
     5881 cgcgacacca acatcaaagc gaaataccag cacgctggtc agcgggaagc cgaagaaaaa
     5941 ggtgagcaaa aacagcaatg aaaagaagac attgaagtta aagcgcacac ggcgaaactc
     6001 aaaccaggtc agcgtggcaa taaacagcgt gcagagcagc cagacaacaa acaggccact
     6061 gaattgcagc agactcatgc gacctccccg gcggcaatcg ccaacgcccg ctgccagcct
     6121 tgtagatagt tagggctaaa gaaggcaatg gtgtttttat ccaccgacgc caactggcgc
     6181 tgcgcttcac gcacaatatc ctcgttgaga tcgtcggtag taaacagcac cggcaaatgc
     6241 tgttccgtca tatcctgcca gaacggattt tcccggttaa gcacacaagg aatgcccgcc
     6301 tgaatcagta agcaaagcgt accaatgccc tgctggcggg caaaaataaa gtaaccaaga
     6361 tcgcactgac gaagtagcgc cagataggcg tcaaattcca gtttttcgct caaaatttgc
     6421 agattttctt cgctgaataa ctccagcccc gcctgacgaa cttcctcaat gtacgcttcg
     6481 ttattaggcg gatatcccat cggcaccacc acttttaccg tatcgccaaa ttgctgatga
     6541 acggcgcaca aggcagcaat atggtcattg ctgcggtcac cggagttccc caccagaatg
     6601 gtcattttcc cttcacgttg ccgatcgttc gccatcgtat tgagagaagg gtccatccgc
     6661 gtcgggaagt acagcagttc gccccgcacc tttggatgcg ttttggcaaa aaagctcaaa
     6721 tcgccgcggg cggcaaatac acagccgact cgcttttgcg ccaggcgacg tagtgggtaa
     6781 aaaagcttat atctcaagcc actggaaagc tcatacaggt ctgcccccca gatatgccag
     6841 aaaaactggc tgggcttaat gccaccactc agcagagcca gccacagtgt gggattgaac
     6901 tgaccgtgga agaaaaaacg ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc
     6961 gccagcgatt ttttcccagg gaaaaattgc acagaaagcg ccggacagct atcacttaag
     7021 ccgtcgtcct tgccaacaac cataaactcg cgcgcgtgct cgctcgtcgc agccagcgcg
     7081 tcattgaaaa accgcaaaac ggttcggtta tggtgaggga tatccgatcc cagtacgtga
     7141 atcagtacag tcatgcccgc ctacgccaga gtaaaaacac gccacaacaa agagaaaaat
     7201 agacgatata agttgccata tatgcctgcg ccgcgcccag tgcaccatgc gcagggatta
     7261 gccagtgggc aaataccatc aataaagtga actggctgat ttccgccaga atataaaacc
     7321 gcaatgacgc tttggcgatc accagataac caaagacata agcgcccact tttaacacat
     7381 cacccactaa ctgccaggca aagagatcgc gcatagcggt aaatttattc gacaacagca
     7441 gccagatagc aaaatcacgc agcagccaga cggtaaaact cgccgccgcc actgccggta
     7501 agacgaattt cagcgattta accacttccc gggtgatatc gcgcttttcc gttagccgcg
     7561 acaacgtggg cagcaaatat acgctgaacg atgccgtaat aaattgcagg taggcatcgg
     7621 aaatactgct caccccttgc cagatcccca cttcatccca gctatactgc gccgccagca
     7681 gtttacgcat catgatgtaa gcaacaggca aggtcaccga cgtaatcaac gccatgagcg
     7741 taaatttgct caactgccct gccagaccgt tatcccagct gggtttcaga tagcttaacg
     7801 ggatgacacc acgtttgatc aacatgatag cggcaggaat taccaccagc gcgggaatca
     7861 gcgccagacc cagcaacgcc ccttcataac cgcccaaacg gtaactgacg tagtacgcga
     7921 gaacgccaat caagctgccg acaatcagcg ataacgcatt acctgcggca tcgcgaaagc
     7981 ctttcatcag cgccagtaac aggttgcccc aggcgatccc catttgcacc agcgccacta
     8041 aacgcaccag cccctgatag tcggtattac caaacaatcc ctggctgatt ggcgcagctg
     8101 ccagcacaaa aaccagcgcc atcagcgtag agaaaccaag taccatcgct gatgaagtgc
     8161 cgaccacgcg gcgcagctgt tgcggattat catggtactg ggcaacgtat ttggttacac
     8221 cgttaaagat gccagctccg gcaagcacgc cgagcacggt aatcaactgg cggaaatttg
     8281 ccgccagccc aagccccgcc ggaccaaatg acacagccag caacttaccg accagtaacc
     8341 cggcaccaat cttgaccagt gtactggccg ccgtccacaa ggacgctttt gccaacgaca
     8401 tatcaggaga agtagttcaa caaagtcgca attaccgtac gctgattgac gggcgacagg
     8461 ttgtagaaca gcggcaggcg cagcaggcgc tcgctctctt tggtggtgta gcgatcttca
     8521 ccgtggaact caccaaagcg ttcccccgca gggcaaccgt gcagcggaat gtaatggaac
     8581 accgccatga tttccgcttc tttcagaaag ttaatcaacg cgctccggtc atcaatatcc
     8641 cgcagtttaa tgtagaacat atgcgcgttc tgcacgcagc catcgggaat cgacggcagc
     8701 tcgatacgcc cggctttcgc cagaggcgct aacgcatcgt agtagttttg ccacagcgcc
     8761 agacgttgct ggttgatacg atccgctgct tccagttgcg cccacaggta cgcagcttgc
     8821 agatcggaca tcaaatagct ggagccaata tcgcgccagg tatatttatc gacctgacca
     8881 cggaagaact ggctgcggtt agtgcccttt tcacggatga tctcggctcg ttcgattaac
     8941 gctttatcgt taatcagcgt cgcgccgcct tcaccacccg ccgtatagtt tttggtttca
     9001 tggaagctaa agcagccaat atgaccaatg gttcccagtg cacgcccttt gtaagtggac
     9061 atcacgccct gagcggcatc ttctaccaca aacagattat gctttttcgc caacgccata
     9121 atggtgtcca tttcgcaggc cacacccgcg taatggaccg gcacgataac gcgcgttttg
     9181 tcggtgatcg ccgcttcaat cagcgtttcg tcgatgttca tggtgtccgg gcgaacatcc
     9241 acaaaaacga tttttgcgcc acgcagcaca aaggcattgg cggtggagac aaaggtgtag
     9301 ctcggcatga tcacttcatc gccaggctgg atatcgagca gcagcgccgc catctccagc
     9361 gaagcggtgc aggacggcgt cagtaacact ttggcgctgc caaaacgttg ctccagccac
     9421 tgctggcagc gacgggtaaa accgccatcg ccacacagtt tgccgctacc cattgccgac
     9481 tgcatatagt cgagttcggt tcccaccacc ggcggtgcgt taaatggaat catgtgatca
     9541 cctgtataac cagtacgcgg tgctttctac attcgcacca ctttgtatgt atcgtttaag
     9601 cgcggcggtg ttgcccattt gggtcgccac ccgcaaagtt gttttaccgc gagcatacgc
     9661 ccagtttagc gccgtttgca tcagctcagc acctacaccg cgtccagcca gcaggccaat
     9721 tcgcgcatct gtcgcattga gttcccgtaa agagacataa ccacgaatac cgccggacgc
     9781 cgcacgtaaa atcagacatt gatgatcaaa ggtgccgcgc acggcatttt caatccactg
     9841 tgcataaaag cgtctgctgg cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc
     9901 aaatgcggcg ctggctaact gacgtaatgc gggaatatcg gtctcttgtg ccactacagc
     9961 accactatca ctgacattgt tcacgggtag tgccaaatca acttcacctt ctaccaggga
    10021 gaatcccagc tgttgcaggg catccagttc acccgtattt gatgccgcaa ttttggcctg
    10081 cacccgtgac cacggcgcta acacgtctgg cgtcaggagc ggtgcttcag acgtaatgcg
    10141 cacgatggcg ctgttaacac caaagaaggc gttttcccag gttagtggct caatactggc
    10201 gcggacgggc acgaagtaac tccagcagat attggccgta gccagttttc gctaatgaac
    10261 tggcagcacg cttcacaccc tcgtcatcga gccagccgtt acgccaggcg atctcttcca
    10321 ggcaggcaat cttaaagccc tggcgttttt ctactgtctg cacaaaggta ctggcttcaa
    10381 tcaggctgtc gtgagtgcca gtgtccagcc aggcaaatcc gcgcccgagc agttcaacgg
    10441 tcaggttgcc cgcctcgagg tacatctggt tgatggaggt aatctccagt tcaccacgct
    10501 ccgacggctt cacctgcttt gcgtactcca cgactttact gtcgtagaaa taaagcccgg
    10561 tcaccgccca gtttgacttc ggctgttttg gcttttcttc cagcgagata gcgcggaaat
    10621 tgtcgtcaaa ctccaccacg ccaaagcgtt ccgggtccat cacctgatag ccaaataccg
    10681 tcgccccttc cgtgcgcgcc gcaacatgac gcagcttcgg actgaagccc tgaccgaaga
    10741 agatattatc gcccagcacc agacaagaag gttcaccgtt gaggaaggtt tcaccgatga
    10801 taaaggcctg cgccagaccg tccgggctgg gctgttcggc gtattccagc tggataccga
    10861 actcactacc atcgcccagc aggcgctgga aataaccttt atcttccggc gtagtgatga
    10921 tgagaatttc gcggataccg gccaacatca gcaccgacag cggatagtaa atcattggct
    10981 tatcgtaaat cggcaacagt tgcttcgata cgccgcgcgt aatcggatgc aatcgggtgc
    11041 cggaaccgcc cgccaagata atacctttca tgtttgcctc cgctgaaaat tagcctttca
    11101 gacctaaacg ctcgccctga tagctgccgt cctgcacctg cttccaccag ctttcattag
    11161 ccagatacca ctgcaccgtt ttacgcattc cactttcaaa ggtttcctgt ggcagccaac
    11221 caagttcacg ggcaattttc gaagcatcaa tggcataacg cagatcatgc cccggacggt
    11281 cagcgacaaa ggtgatcaag tcacgatagt gcaccacgcc gtgcggcttg ttcggagcca
    11341 gttcttccag cagctcgcaa atggtttcca caacatcgag attcttacgc tcgttgtgac
    11401 caccaatatt ataggtttca ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg
    11461 cgtgatcttc cacatacagc cagtcacgga tttgctgccc gttgccatat accggcagcg
    11521 gtttacccgc cagcgcgttg aggatcatca gcgggatcag tttttccgga aagtggtaag
    11581 gaccgtagtt attcgagcag ttggtgatca gcgtcggcag accgtaggtc cgcagccagg
    11641 cgcgcaccag atggtcgctg ctggcttttg acgcggaata agggctgctc ggcgcatacg
    11701 gcgtggtttc ggtgaagaaa tcatccgttg agtgcaggtc accatatact tcgtcggtgg
    11761 agatatgatg aaaacggaac gctgattttt tatcttccgt cagcgtattc cagtaagccc
    11821 gcgccgcttc aagcaatgta taagtcccga caatgttggt ttcaataaac gctgccgggc
    11881 cgtcaataga acggtcaaca tggctttcgg ctgccagatg catgacacag tctggctgat
    11941 gctcagtgaa tacgcgtgcc agttctgccc gatcgcagat atcaactttc tcaaaggcaa
    12001 agtgctcgct ttgcgcgacc ggtgccagcg acatcaggtt tccggcgtag gtcagcttat
    12061 cgaccactac caccgcatcg ctcgtttcgt tgatgatata acgcaccagc gccgagccaa
    12121 taaacccggc accacctgtt atcagaattt ttctcatcag cgccagactc ctttggcatc
    12181 gacgacatac tgctgatgga cattgtcgcc attgataact ttgaactgac tatgatcgac
    12241 cagcatcacc agcacgtctg ccgttgccag cgcctcgtca agctgcgcca gagtacaaag
    12301 cccggtcagt tttttcggca actggtggat gttaggctca acaaccagcg tttcgccgct
    12361 gtgccactgg gcgatcagtt cagcgatttc catcgccggg ctttcgcgca ggtcatcaat
    12421 attcggttta aacgccagac caaagcaggc gattttcagt tcactggcgc gtttatcggt
    12481 agcagcaagg cagtcggcaa ccgccgcttt cacctgatcg ataacccaga acggtttgtg
    12541 atcgttaact tcgcgcgcgg tacggataag ccgcgcctgc tgggggttct gtgccacgat
    12601 aaaccacgga tcaacagcaa tgcagtgacc gcccacgcca gggccaggct gaagaatatt
    12661 aacgcgaggg tgacgattcg ccaggcgaat cagttcccag acgttaatcc cctgatcggc
    12721 acaaatcagc gacaattcat tagcaaaagc gatattcaca tcgcggaagc tgttttcggt
    12781 gagcttacac atttccgccg tccgcgagtt agtgacgaca cactcacctt cgaggaaaat
    12841 tttgtacagt tcgctggcgc gggtcgaaca aaccggcgtc ataccaccaa tcacgcgatc
    12901 gtttttaatc agctcgacca taacctggcc cggtaacacg cgttccgggc agtaagcaat
    12961 gttgacgtcc gcctgctcgc ccacctgctg tgggaaagtg agatccggac gcatctctgc
    13021 taaccattct gccatcttct cggttgaccc caccggcgag gtggattcaa ggatcaccag
    13081 cgcgcctttt ttcagcactg gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc
    13141 tggctcatga tcgcctttaa acggcgtggg tacagcaatc agccaggcat ccgcttcaac
    13201 tggcgtcgtg ctcgctcgta aaaaaccgcc ttctacggca gtttttacta cgctcgccaa
    13261 atcaggctcg acgatatgga tttcgccacg attgatggta tcaaccgcgt gttggttgat
    13321 atcgacacca atcacctgtt tttgccgtga ggcaaacgct gctgctgtag gcagcccgat
    13381 gtaacccagt ccgataacag aaatggtcgc aaaactcata gtgatatccg attatttttt
    13441 aacgcttcca gaatgcgaga gcatgcctga ccatcaccat acgggttatg ggcgcggctc
    13501 atagtttgat attcgttttc gtcttttaaa agacgcgtca cttcctcgac aattcgctgc
    13561 ttatccgtgc ctaccagacg caccgtaccc gccgtcaccg cttccggacg ctcagtggta
    13621 tcgcgcatca ccagcaccgg tttccccagc gaaggcgctt cttcctgaat gccgcctgag
    13681 tcggtcaaaa tcagccaggc gtggttcatc agccagacaa acggtaaata ctcctgggga
    13741 tcgatcagaa tgacattttt cacatgcccc agaatgcgat tgaccggctc tctgacgttc
    13801 gggttgagat gcaccggata gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc
    13861 agcgcgtggc agatttcttc aaagccacga ccgaaacttt cacgcctgtg accggtcacc
    13921 agaatcatct ttttatcggg gtcgataaac gggtaatttg ccgccagttc tgaacgcagc
    13981 gtgtcgctgc tcatcacctg atcacgcacc cataacagtg catcaatgac tgtattacca
    14041 gtaatgaaga ttcggctatc cgcaacgttt tcacgcagca agttttgccg ggaagtttcg
    14101 gttggagaga agtgatacat cgccagatgc ccggtcaatg tacggttagc ctcttccggc
    14161 cacggcgaat agagatcgcc cgtgcgtaaa ccagcctcaa cgtgaccaac aggaatacgc
    14221 tgataaaacg ccgccaggct ggttgccagc gtcgttgttg tgtcgccgtg aaccagcacg
    14281 acgtctggtt tgaactcggc aagaataggt tttagccctt ccagaatccg acaggttatc
    14341 tctgtcaggc cctgtcctgg ctgcattatg ttgagatcgt agtcaggtac aatggaaaag
    14401 agtttcagca cctgatcgag catctcccga tgctgcgcag tgacgcaaac tttagcctca
    14461 aaaaaaggat cttttgccaa cgcatgcacc aacggagcca tcttgatggc ttccgggcgc
    14521 gtaccaaata cagtcagtac tttcacatcg attctcttcg aataagcggc gagcgccttt
    14581 gcgctcgcag cagcagtgtt gctatttcga gcaacggcgg gttaatgcga caccagcccc
    14641 gatcagcccc ccgacaatgc cccacataat catcaggaag gcacgacgtg ggctatcgcg
    14701 ttttaccggt tcttccggcg tacgcaaata gcgataggtc tgaaaacgcg gatccagggt
    14761 tggaccaaca ttcagggtgt ttaacatggc ccgattctga tcatagtcga gatcaaaggc
    14821 cggaccgacg gcctgtaaat tttccagtcg agcctggagc attggacgcc caagcaggaa
    14881 catttctgaa tcaggtaatt cctcggcagg cacatctgtc gcactgcgcg aaatattatg
    14941 ctgctcagca attttcagcg cctgctcaat gctgttcatc cggcggtcgt agatggcttt
    15001 cgccacctct tcctgacgct tcacctgagc tttcatctgg atggtgcgtg ccgcccatgc
    15061 gcctttcagc tcatcattca gatggctggc tgcacgctgg ctggcaaaag caacatactg
    15121 acgtaacagg ttattagcgt caggcgcggt ttcggcaata agcttcacgc tgtcattgac
    15181 cgcgcgggta aagtctccgg ggataaactg gatgttgtta atcatttcat ccagcaacgc
    15241 cgcatcggct ttgctgttgc ccaccatccg ctgcttgtaa tagtcggttt gcagccagaa
    15301 ctctctgcgg gtatcccacg aggccagttg cataacaaac tctttatagg cttcgtccat
    15361 gaccgatggt tggtcggcag aagccatgtt tgaacgggca tccaggttac gcaaaaattg
    15421 ctgctgcgag taatatcccc ccagcatatt caccgttgga cgatcggtaa tcgccgtcgc
    15481 gctccactct tgtcgagcaa aaaaagtata cgccagcgcg attaacgcaa acgccagccc
    15541 catgccaata atccatagct tcccagccca caaggtacga aacaacccac gaatatccag
    15601 ttcattttca gcgtcttcgg ccggtttccc aggcattggt tgtgtcatca catcctcatt
    15661 tatttggtta aattggggct gccaccacga tttctacgca gtctgcgttt tacgcgctta
    15721 ataaagcgag caactttcca ggcacgctta atgcaatatc catagaggaa gaatgctagc
    15781 aaaaagagca ccagcatgac ccactccggg acaaaatgag aatattctgc cagcacgcca
    15841 atggaagcga gcagtgctgc ggcaagggta atcagcacaa acgcctgacg ggaagtaaac
    15901 ccggcacgca tgatcaaatg gtgaatatgc tgacggtcag gagagaatgg gctcatgcct
    15961 ttacgcaggc gacggtacat aatcgccacc atatccatta gcggaatggc gattatccac
    16021 aaagcggtaa ccgggctgat gggatgggtt ttgccctggg tcgtttcgag caggatccag
    16081 ataacggtaa aaccaatcag cgtactgccc gcatcaccca taaagacttt gtagcggcga
    16141 cccaggatac caaggttaag catgatgtat ggcaggatgg cggcgatcat cgcaaagcac
    16201 cagattgcga ggctggtttg cccgtcgaac cacaaaatca taccgattgc tgcaaacgag
    16261 acgcaggaca acccgcccag caagccatca atgccatcaa ccatgttgaa cgcattaatg
    16321 gccgcccaga cggcaaatag cgtcaggaag taaccaaacg gtccgagcac catctcccag
    16381 gagccaaaga tataacccag gctactgaga taaagtttgc cgaacaccat cataacaatg
    16441 ccaacagcgg cctgtatggt ggcacggatt tttacgctga tatcaaaacg gtcatccagc
    16501 gcgccaatga aaacaagcac accggcacaa gcgagataga gagatgcatg cggaatatag
    16561 taatcgacaa ttccgaacgt gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga
    16621 ggtatcaatc cctggtgacg tttgcggaag tttggtttat ccactaaacc gacttttttt
    16681 gccactttac gggcaaaaaa cagaaacagt gtcgtgaata aaaaaatact gatgagatca
    16741 gtactcactg tcagtaaatt cacaatgcat gctctcagag aaaattatta gcagaagtat
    16801 aaccacgaag acctttattc agaagggaaa gtccgttccg aacccaccag tcctgtgcaa
    16861 aaattaacca tttgatatgc aaattgcata acatgccagc aaattccagg aattttccta
    16921 acatcgccag cgcggcatta agattacaga tataaaagca aaacgccacg taaacacgtg
    16981 gcgtttttgg cataagacaa atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg
    17041 tcttagtcat ggccagttta ttaatgagga attccattgc atcgatttcg cccatcgggt
    17101 gaatgatttt gcgcaggatc cacattttct gcagttcttc ctgagtcgtg agcagctctt
    17161 ctttacgggt accggaacgg ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct
    17221 tacgagagag gtgcagttcc atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt
    17281 ccattttaga accggtatcg ataagcgccg tcgcgataat ggtcaggctg ccgccctctt
    17341 ccacgttacg cgccgcaccg aagaagcgtt tcggacgatg aagggcattg gcgtccacac
    17401 caccggtcaa cactttacct gacgccggaa caacagtgtt gtaagcgcgc gccagacgag
    17461 tgatggagtc gagcagaatg ataacgtctt tcttgtgctc aaccaggcgt ttggccttct
    17521 cgatcaccat ttccgcaacc tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa
    17581 caacttcacc ttttaccaga cgctgcatct cggttacttc ttccggacgt tcgtcgatca
    17641 gcagaaccat cagcacacaa tccgggtggt tgtaagcaat gctctgagca atgttctgca
    17701 gcagcatagt tttaccggct ttcggcggtg ccacaatcag accacgctga ccacgaccga
    17761 taggtgatgc cagatccagt acgcgagcgg ttaaatcttc agtagaaccg ttaccacgtt
    17821 ccatacgcag acgagagttt gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc
    17881 gggcgttttc aggtttgtcg aagttaactt cgttaacttt cagcagcgca aaatagcgtt
    17941 caccttcttt cggcgggcga atcttaccag agatggtatc accagtgcgg aggttgaaac
    18001 ggcggatttg gctaggggaa acgtagatgt catcaggacc ggcgaggtag gagctgtctg
    18061 cggaacggag gaaaccaaat ccatcctgca atatctccag tacgccatca ccaaagatat
    18121 cttcgccact ctttgcgtgc tgcttcagga tggcaaaaat aatgtcctgc ttacgcatac
    18181 gagccaggtt ttccagcccc atattttcgc cgagagtgat cagctcagaa accggcgtat
    18241 tctttaattc ggtaagattc atagtggtgt gagttcttaa acttggggta aatctcgaac
    18301 ttaatgttgt gaatggtatg gcaggatcat ccatgcctgt ttacggtcat cgactcatgt
    18361 cttttcgctg cctggtcaca ggagagtacg cagaactgaa acgacaagac ggattgagtg
    18421 acgagccaga aatctgttca cttcgcattt aagataaaac gggaagcgtt ggaaattaca
    18481 agattcaaac ttaataaggt atgtttaata cgaagtcaac actaagttag catgactcac
    18541 gccgggcgtc cagtttttag cgacggggca cccgaacatg aaattccctt acgccaggtt
    18601 agcgtcgagg aactctttca actgaccttt agacagtgca cccactttgg ttgccgccac
    18661 ttcaccgttt ttgaacagca gcagagtcgg gataccacgg atgccatatt tcggcgcagt
    18721 gccagggttt tgatcgatgt tcagttttgc aacggtcagt ttgccctgat attcgtcagc
    18781 gatttcatcc agaatcgggg cgatcatttt gcacggaccg caccactctg cccagaaatc
    18841 gacgaggatc gccccgtccg ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg
    18901 aataatttta tcgctcatat ataactccac aggaataagc ctggcgtgtt ggtttcgttg
    18961 ttggtgtaac attaaccaac taaaggttga ctttatttca ccggatacgc tttcgtaaag
    19021 caatagtaag ctgatattct accacactat gagcaaaaca catttaacag aacagaagtt
    19081 ttccgacttc gccctgcatc cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa
    19141 ctgtacgccc attcaggcac tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg
    19201 gcaggcgcaa accggtaccg ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct
    19261 tctctctcat cctgcgattg ccgatcgcaa ggtgaatcaa ccacgtgcct taattatggc
    19321 accgacgcgt gaacttgccg tgcagatcca tgccgacgca gaaccgctgg cggaagctac
    19381 cggcctgaag ctgggtctgg cttacggtgg tgatggctac gacaaacagc tgaaagtgct
    19441 ggaaagcggc gttgacattc tgattggcac tacggggcgt ttaattgact acgccaagca
    19501 gaaccacatt aacctcggtg ccattcaggt ggtggtactg gacgaagccg atcgcatgta
    19561 cgatctgggc tttattaaag atatccgctg gctgttccgc cgtatgccgc ctgcaaacca
    19621 gcgcctcaac atgctgttct ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga
    19681 gcagatgaac aatgccgaat atattgaagt ggaaccggaa cagaaaacgg gccaccgtat
    19741 taaagaagag cttttctacc cttctaacga agaaaaaatg cgtttgctgc aaacgctgat
    19801 cgaagaagag tggccagaca gagcgattat tttcgccaat accaaacacc gttgtgaaga
    19861 gatctggggc cacctggcgg cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc
    19921 gcagaaaaaa cgtctgcgta ttcttgatga atttacccgt ggcgatctgg atattctggt
    19981 tgccaccgac gttgccgcgc gtggtttgca tattccggca gtgacgcacg tctttaacta
    20041 cgatttaccc gatgactgtg aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg
    20101 cgcaagcggt cactctatca gcctggcgtg tgaagagtat gcattgaatt tgcctgctat
    20161 tgagacctat attggtcact caattccggt aagcaaatac aatccggacg cattgatgac
    20221 cgatctgcca aaaccgctgc gcctcacgcg cccacgtaca ggcaatggtc cgcgtcgtac
    20281 tggcgctccg cgtaatcgtc gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc
    20341 aaactgtatc aagatggcaa gagaatgaat cccgatgagc atactgtagt aagtgattca
    20401 ggtgaaagaa tgcagccaac acagagacag attgaaggat gaagagtatg ggttccacct
    20461 cgtcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg
    20521 aggtggctgg aagcatccag acgctgacgc gaataaaacg caaagtgcgt ctggctgctg
    20581 gcctgaatag cgaaaatgcc ctgtctaatg aagcaatgga gcgcggttgg caatgtctgc
    20641 gcctgtttgc tgaacgtctg caagatatcc ctccctcgca aattcgcgtt gtcgctacgg
    20701 cgacattacg tcttgccgtc aatgcgggtg attttattgc caaagcgcag gaaatcctcg
    20761 gttgtccggt acaggtgatc agcggtgaag aggaagcacg tctgatttat cagggcgttg
    20821 ctcacacaac tggcggtgcc gatcagcgcc tggtggtgga tataggcggt gccagtactg
    20881 aactggtaac cggcacgggt gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg
    20941 tcacctggct ggaacgctat tttgccgatc gtaatctggg gcaggaaaat tttgatgctg
    21001 cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg tatcacggct
    21061 ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt acaggaaatc atgatggcac
    21121 aggggatgga tgaacgcatt accctggaaa agttgcagca attgaaacag cgagccattc
    21181 attgcggtcg gctggaggaa ctggagattg acgggctgac gctggaacgt gcgttagtgt
    21241 tcccgagtgg tctggcgatc ctgatcgcca tttttaccga actgaatatt cagtgtatga
    21301 ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg
    21361 agcaggatat tcgcagccgt acgctgcgta atattcagcg ccgctttatg atcgatattg
    21421 atcaggcgca gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg gaaaaggaat
    21481 ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg
    21541 gcctgagcgt tgactttaaa caagcgccgc aacacgctgc ttatctggtg cgtaatctgg
    21601 atcttcccgg ttttaccccc gcacagaaaa aattgctggc gacgctactg ctcaaccaga
    21661 ctaatccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccgccg cgcgtcgcag
    21721 aacaactctg ccgtttactc cgcctggcca tcatttttgc cagccgtcgc cgtgacgatc
    21781 tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg cttccgcaag
    21841 gttggctaac ccaacatccg ctgggtaaag agattattgc tcaggaaagc cagtggcaga
    21901 gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc gatgctgacg
    21961 catcttttcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt tcagattcgc
    22021 cagatggctt tgccctttct gcatccgttc ttcggcgctg accactttgc gctcctgttc
    22081 ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg ctcggctccg ggcgcaccag
    22141 ctcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct gggcgcgggt
    22201 aatgccgaca taggccagcc gccgctcctc atcgatatta tcttcatcga tgctgctctg
    22261 gtgcggcaaa aacccttctt ccataccgac catgtagaca taaggaaact ccagcccttt
    22321 tgacgcgtgc agagtcatca gttgcacctg atccagctct tcttcactct caccacgctc
    22381 catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg agcgtcatcg gctcatccag
    22441 ttcgctgcct tccagcatct ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg
    22501 catttcggcg gctttcgggc tgggcgatgt ttcgtacagc caggattcat aatccatgcc
    22561 gtggatcaga tcgcgcaccg cggcaatcgg ctcccgctcc gccagacgct ggatttctgc
    22621 caaccagtga gtaaagcggg tcaatgcttc ataaccacgt ccgctaagcg tctgactcag
    22681 gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc actcacccag
    22741 ctttttcagc gtcgccgggc caatctctct cttcggcgtg ttaacgatac gcagaaatgc
    22801 gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct tgatttcagg
    22861 acgagagaaa aacgacgtac caccagaaat tttgtacggg atgcggtttt gcatcaggaa
    22921 tttttcaaac actcgcgact gatggttacc gcgataaaga atagcgtaat ctttgtactg
    22981 cgttttattg acgaagtgat gcgcaatcag ttcaccagtg acgcgctcgg cttcatgttc
    23041 ttcgttattc gcgcttaaaa ctttaagctc tgtgccgtaa cccagttcgg agaacagacg
    23101 cttctcaaag acgtgcgggt tattggcgat caggatgttc gccgctttca aaatacgtcc
    23161 ggaagagcgg tagttctgct caagcttaat caccgtcagc gccggaaaat cctgactcag
    23221 cagcaccagg ttttgtggac gtgcgccgcg ccaggagtag atcgactggt cgtcatcgcc
    23281 caccacggta aagcgcgcgc ggctgcccac cagcagtttc accagctcat actggctggt
    23341 gtttgtgtcc tgatactcat ccaccagcag atagcgaatt ttgttctgcc agcgctcgcg
    23401 gacttcttca ttgcgttgca gcagcaacgt cggcaataaa atcagatcat cgaagtcgag
    23461 aacattgcac gctttcaggt gtgcatcata cagcccataa caatgggcaa aaatacgatc
    23521 ccgctcgccc tttgcctctg ccgccgcctg cgccggagtt ttgagatcgt tcttccagtt
    23581 ggagatggtc gaaatcagct gttgcaggag aactttgtca tcttcaatca gcccctcggt
    23641 caactcttta agcaaagcaa gctgatcggt atcgtcaaac agcgaaaagt tcgccttcat
    23701 cccaagcgcc gcatactcgc gtttgatgat atccaacccc agcgtgtgaa aagtggagat
    23761 catcagcccg cgcgcctctt tgcgacccag cgtctgcccg acacgctctt tcatctcgcg
    23821 cgccgcctta ttggtaaagg tcaccgccgc gatatgtcgc gcctgataac cgcaaccgcg
    23881 gatcagatgg gcgattttat tggtgatcac acgagtttta ccggaacccg cgcccgccag
    23941 caccaggcag gggccggtaa cgaattcgac agcttgttgt tggccggggt ttagacgcat
    24001 aggtgtattg ctcaatcttc gaacgggggg aggattgtag cagaatcgca tttgaatgtg
    24061 accgcgtgta agccgctaac cggttggctg cggatgttta aatcagagag agaagaccga
    24121 aatcggagtt catcgtgaat ccattagact tagaaaatat cgggtcgtaa cgttgtgtta
    24181 ttactttttc agagtttact aaggagaggt ttactcgtgg cgattaattt cagccctaag
    24241 gttggtgaaa tacaggaatg caactttggt aactatcctg ttatccagaa tggacagttc
    24301 agcaccacgt attacgatgg ccgcattcct cctgaaatga taaaaaacag gttagttgtc
    24361 gtgctcaacg gaaaaattaa tggcaacgca tgtatcgtcg ttccattatc aaccactcgc
    24421 gatcatgaca agttaaatcg tggaatgcat gttgaaatag ccagtaatgt tatcaatgtc
    24481 ctgcaattct ttgatcagca gattcgttgg gccaaagccg acctggtaca acaagtcagt
    24541 cgaaaccgat taaatagagc aaggacttac agagggtatc ttaatcaatg tttgccacat
    24601 gagttggttg cagacataca gagggcggtc atcaagtcaa tcaatgccat atcgctcata
    24661 aattgacaat aaaataacca tccccgataa tgactttgta gcccatagag gccctaagtt
    24721 ttctcccctt gagggacctg cgaagcccgg attttccggg cttttgccat atatgtacca
    24781 gcccctccct gactgacaat tacgttagca tctgcggatc atgctaaaat cgccgcctga
    24841 caattattca ctcaaggcac gatcatggca aaaacagcag cagcactgca tatccttgta
    24901 aaagaagaga aactggctct ggatcttctc gagcagatta agaacggtgc cgatttcggc
    24961 aagctggcga agaaacactc catttgccca tcaggcaaac gcggcggtga tttaggtgaa
    25021 ttccgccagg gtcagatggt tccggcgttc gataaagttg tgttctcttg tccggtactg
    25081 gagccgaccg gcccgctgca cacccagttc ggttaccaca tcattaaggt gctgtaccgc
    25141 aactaatagc aaggccttct ccaggagaag gccttgagtg ttttctccct ctccctgtgg
    25201 gagagggtcg gggtgagggc atcagcgcgc acttaacccg caacagcaat acgtttcata
    25261 tctgtcatat agccgcgcag tttcttacct acctgctcaa tcgcatggct gcgaatcgct
    25321 tcgttcacat cacgcagttg cgcgttatct accgcgcctt ccggaatagc tttacccaag
    25381 tcgcccggtt gcagctctgc cataaacggt ttcagcaacg gcacacaagc gtaagagaac
    25441 agatagttac cgtactcagc ggtatcagag ataaccacgt tcatttcgta cagacgctta
    25501 cgggcgatgg tgttggcaat cagcggcagc tcgtgcagtg attcataata tgcagactct
    25561 tcaatgatgc cggaatcgac catggtttcg aacgccagtt caacgcccgc tttcaccatc
    25621 gcgatcatca gtacgccttt atcgaagtac tcctgctcgc cgattttgcc ttcatactgc
    25681 ggcgcggttt cgaaggcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca
    25741 tcgttggccc agtccgccat cataccggag gagaactcgc cggagatgat gtcgtccata
    25801 tgtttctgga acaggggtgc catgatctct ttcagctgtt cagacagcgc gtaagcacgc
    25861 agtttcgccg ggttagagag acggtccatc atcagggtga tgccgccctg cttcagcgct
    25921 tcggtgatgg tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc
    25981 tcttccacca gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg
    26041 gtttgctcac ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc
    26101 gcacggtgac caccggttgc tgccgcccat gctttagcga tcgccatacc ttcgcctttc
    26161 ggatcgtttt ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct
    26221 tcacgcactt cggtgccagg gcatttcggc gcaaccatga ctacggtgat atctttacgg
    26281 atctgctcgc ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct
    26341 ttcatcagtg gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta
    26401 accaccagat ccgcctgcgg gatcagttct tcgtaagtac caactttaaa accgttttcg
    26461 gtcgctttac gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag
    26521 atatcgagac cagaatcacg catgttcagg ccctggttca gcccctgtgc gccacagccg
    26581 acgataacca cttttttacc ctgaaggtag ctcgcgccgt cggcgaattc gtcgcgcccc
    26641 ataaagcgac atttgcccag ctgtgccagc tgctggcgca ggttcagtgt attgaagtag
    26701 ttagccatgg tgattcctcg tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga
    26761 ttgaattcac tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat
    26821 tgcaatttct gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac
    26881 cttcctgcat ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag
    26941 cccatccacg ctctcacggc agattcagcg cctggaagaa gatcttggtc agccgctatt
    27001 tgtgcgcgat aaccgcacgg taacgctgac cgaagcgggc gaagagttac gcgttttcgc
    27061 ccagcaaaca ttgttgcagt atcagcaact gcgccacacc atcgatcagc aagggccgtc
    27121 gctctctggc gagttacata ttttctgctc ggtgaccgcc gcttacagcc atctaccgcc
    27181 gattctcgac cgcttccgcg cggaacaccc gtcggtggag atcaaactca ccactggtga
    27241 tgcggcagat gcaatggaaa aagtggtcac gggagaagcg gatctggcga ttgccgggaa
    27301 accggagacg ctacccggcg ctgtggcgtt ttcgatgctg gagaatctgg cagtagtgct
    27361 gattgccccc gcgctgccct gcccggtgcg taatcaggta tctgtcgaaa agcccgactg
    27421 gtcaacggtg ccgtttatta tggccgatca ggggccggta cgccgccgca ttgaactgtg
    27481 gtttcgacgc aataaaatca gcaacccgat gatttacgcc acggttggcg ggcatgaagc
    27541 gatggtatcg atggtggcgc tcggctgtgg tgtggcgctg ttgccggaag tggtgctgga
    27601 aaacagcccg gagcctgtac gcaaccgggt aatgatttta gatcgcagcg atgagaaaac
    27661 gccgtttgaa ctgggcgtgt gtgcacaaaa aaagcggctg catgagccgc tgattgaggc
    27721 gttctggagg attttgccga accacaaatg acgttgtcgc gcgggtaggc ctgataagcg
    27781 aagcgctatc aggcattttt ccctaacccg ccaaaaagaa cctgaacgcc ggattattgg
    27841 tttcgtcgtg gcaatcgtag cccagctcat ttagccgggt ttcgaaatcc ggttcatggt
    27901 cgccgagttc gaacgccgcc agtacgcgcc cgtagtcggt gccatggctg cgatagtgga
    27961 acaaagaaat gttccagtgc gtacccagcg tgttgaggaa gcgcagcagc gcgcccggtg
    28021 attccgggaa ttcgaagctg tagaggcgtt cctgcaacgg atgtgacgga cgcccgccga
    28081 ccatatagcg cacatgcaac ttcgccattt cgtcgtcgga gagatccacc acgctgtagc
    28141 cgccgtcgtt gagcatctgc aaaatttctt tgcgctcttc gaggccgcgg ctcaggcgca
    28201 cgccgacaaa gatgcaggcg tttttggcat cggcaaaacg gtagttgaac tcggtgaccg
    28261 atcgcccgcc aagcagttgg cagaatttga ggaagctgcc tttttcttcc ggaatggtca
    28321 ccgccaacaa cgcttcacgc tgttcgccca gttcgcagcg ttccgagacg tagcgcaggc
    28381 cgtggaagtt cacgttggca ccggaaagaa tatgcgccag ccgttcgccg cgaatgttgt
    28441 gctgggcgat atattttttc attcccgcca gcgccagcgc gccagagggt tccgccaccg
    28501 cgcgcacatc ttcgaataaa tccttcatcg ccgcacagat cgcatcacta tcgacggtga
    28561 tgatgtcgtc gagatactcc tggcataaac ggaaggtttc gtcaccgatg cgttttaccg
    28621 ctacgccttc agcaaatagc cctacgcgcg gcagatcaac cggatgaccc gcatccagcg
    28681 ctgctttcag gcaggcggag tcttccgctt ctacggcgat cactttgatt tgtggcatca
    28741 gttgtttgat cagcaccgca acgcccgccg ccagaccgcc gccgccgact ggcacaaata
    28801 cgcggtcgag atgggcgtcc tgctggagca gttccagcgc cagcgtgcct tgcccggcaa
    28861 tcaccatcgg atggtcgaac ggcggcaccc aggtgaaccc ctgctgctgt gacagttcga
    28921 tcgctttagc tttcgcttcg tcaaagttcg cgccatgaag cagcacttcg ccgccgaatc
    28981 cacgtaccgc atcgactttg atgtcggcgg tggcggttgg cataacgatc agggccttca
    29041 cgcctaaccg cgcagaagaa aacgcgacgc cctgcgcgtg gttacccgca gaagcagtga
    29101 tcacgccgtg cgctttttgt tcttccgtca ggcccgccat catggcgtat gcgccgcgca
    29161 gcttaaagct gtgcactggc tggcgatctt cgcgcttcac cagaatgacg ttatcaagac
    29221 gcgacgacag tttttccatt ttttgtagcg gcgtaacctg cgccgcctcg taaaccggcg
    29281 cgcgcagcac tgctcttaaa tattcggcac cttccggagc accggacagg ggttgcgagt
    29341 cagccattat taacccccca gtttcgattt atcgcgcacc gcacctttgt cggcgctggt
    29401 tgccaggctg gcgtaggcgc gcagggcaaa ggaaacctga cgttcacggt ttttcggcgt
    29461 ccaggctttg tcaccccgag cttcctgcgc ttcacgacgc gccgccagtt cggcatcgct
    29521 tacctgtaac tgaatgccac ggttcgggat gtcgatagcg atcaggtcac catcttcaat
    29581 cagaccaatg ctgccgccgc ttgccgcttc cggtgagacg tggccgatgg aaagaccaga
    29641 ggtgccgcca gagaaacgac cgtcggtgat cagcgcacag gctttaccga gacccattga
    29701 tttcaggaag ctggttgggt agagcatttc ctgcatgcca gggccgcctt tcgggccttc
    29761 atagcggatc accaccacat cgcccgcgac aactttgcca ccgaggatcg cttctaccgc
    29821 gtcgtcctgg ctttcgtaca ctttcgccgg gccggtgaat ttgaggatgc tgtcatcgac
    29881 gcccgcggtt ttaacgatgc agccgttttc tgcgaaatta ccgtagagca ccgccaggcc
    29941 gccgtctttg ctgtaggcgt gttccagcga gcggatacag ccattggcgc gatcgtcatc
    30001 gagagaatcc caacggcaat cctgcgagaa tgcctgtgtg gtacgaatgc ctgcaggacc
    30061 tgcgcggaac atatttttta ccgcgtcatc ctgggtcagc ataacgtcgt attgttccag
    30121 cgtttgcggc aacgtcaggc caagtacgtt tttcacatca cggttcagta accccgcgcg
    30181 atccagttcg ccgagaatac cgataacacc accagcacgg tgaacatctt ccatatggta
    30241 tttctgggtg ctcggcgcaa ctttacacag ctgtggaacc ttgcgggaaa gcttatcgat
    30301 atcactcatg gtgaagtcga tttccgcttc ctgcgccgcc gccagcaggt gaagtacggt
    30361 gttagtcgat ccacccatcg cgatatccag cgtcatggcg ttttcaaacg ccgctttgct
    30421 agcaatattg cgcggcagtg cactttcgtc gttttgctcg tagtaacgtt tggtcaattc
    30481 aacaatgcgt ttaccggcat tgaggaacaa ctgcttacgg tcggcgtggg ttgccagcag
    30541 cgagccgttg cccggctgcg acagacccag cgcttcggtc aggcagttca ttgagttagc
    30601 ggtaaacatc ccggagcagg agccgcaggt cggacacgcg gaacgttcaa cctgatcgct
    30661 ctgggagtca gaaactttcg ggtctgcgcc ctggatcatc gcatcaacca gatcgagttt
    30721 gattatctga tcggacagtt tggttttccc ggcctccatc gggccgccgg aaacaaagat
    30781 caccggaata ttcaggcgca gggaagccat cagcatcccc ggggtgattt tgtcgcagtt
    30841 agagatgcag accatggcgt cggcgcagtg ggcgttgacc atatactcaa cggaatcagc
    30901 gatcagttcg cgagatggca gtgaataaag catccccccg tggcccatgg caatcccatc
    30961 atccaccgca atggtgttga actctttggc aacgccgcca gccgcttcaa tttgttcggc
    31021 gaccagttta ccgagatcgc gcagatggac gtgacccggt acaaattggg tgaacgagtt
    31081 cacaaccgcg ataatcggct taccgaaatc ggcgtcggtc attccggtgg cgcgccacag
    31141 cgcacgagca cccgccatat tacgaccatg agtggtggtg gcggaacggt acttaggcat
    31201 actttattta ctcccagtgt ctgtctcgta aatgggacgg tgcgtgccgt cccatttttt
    31261 gtatttattg attaacttga tctaaccagc cccatttatc ttcggtttcg ccagtgaaga
    31321 ggccgaagaa ggcttgctga atgcgtttgg taaccgggcc acaacggcct tcgccaacct
    31381 gaataccgtc tacgctgcgc actggcgtga tttctgccgc cgtaccggac ataaacactt
    31441 catccgccag gtacagggat tcgcgcgaca gaacctgctc acgcacatca attcccagct
    31501 ctttcgccag tttgatgatg gcatcacggg taatacccgg cagcgcggag gaggtgaacg
    31561 gtggggtgaa cagcacacca tctttcactt caaacaggtt ttcgcctgcg ccttcagaga
    31621 tataaccgtt cacatccagc gcgatacctt cctgataacc gtggcggcgc gcttcgctac
    31681 ccaccagcag ggaagagagg tagttaccac cggcttttgc cgccgtcggg atggtgtttg
    31741 gtgctgcgcg gttccaggag gaaaccatcg catcgatccc ctgctccagc gcttctgcgc
    31801 ccagatacgc tccccacggg aaagcagcga taatcacgtc ggttgagtat cccgctggcg
    31861 ggtttactcc catgccaaca tcaccgacga agatcagcgg acggatatag gcgctggtga
    31921 gattattttt gcggatcacg tcacgacaag cttccatcag ctcatcaatg ctctgtgaaa
    31981 ccgggaagcg atagattttg gcggagtcat gcagacgctg catatgctca cgatggcgga
    32041 atacaaccgg tcctttgtgt gagtcgtagc aacggatgcc ttcaaaaacc gaggtgccat
    32101 agtgcagcgc gtgcgacatc acatgcacct tcgcgtcttc ccagcgaacc atctccccat
    32161 tgaaccaaat gtaatcagct ttcttcgtgg tcatttttat attccttttg cgctcaggcg
    32221 cggatttgtt gtgatgtggt tgtgctctgg cagatggcaa cgtgtgcgac gtccaccagt
    32281 ttatttaact gactaaacag taagtcgacc gaccgtgggc tggcaacggt caattcgata
    32341 tttatatttt gtgcatcgct ggcggcagcc atattcattg agcagacgtg gaaaccacga
    32401 tgacgcacca cgcgtaaaac acgttctaag gtttccggat tgaagcgagc cgatacattg
    32461 acctgatgtt gcatcatgat aatttctcca acatttctga attactggcg ccaggcggca
    32521 ccagcggcca gacgttctca agttcgtcga ttgagacatg aagcaggtat ggcccatcac
    32581 tgttcagcat ggtgtcgagt gccgcttcaa cctggtcttt acgggtgatg tgttggccag
    32641 ggatgccgaa ggcgctggct aacatgagga aatcggggtt atcagtaagg gtggtttcgc
    32701 tgtatcgttc ctgaaaaaac agttgctgcc attgtcgaac catccctaac cgttggttat
    32761 cgagtaagac gattttcaac ggtaactgct tgcgttttac ggtgcccagc tcttgcacat
    32821 tcatcatgaa agagccgtca ccggagatac agacgacagt atcgttcggt cgtgcgactt
    32881 gtgcgccaac cgccgctggt aaaccgaaac ccatggtgcc taagccgctg gaggtaatga
    32941 aattttccgg gcgagtgtgt gcgatgtgct gcgcggccca catctggtgc tgccccacat
    33001 ctgtggtcac gacgcaatcc gcaggtttac gatccgacag ttgttttaac aacaatggcg
    33061 cgtagatagc gtcaccggga tggtcgtaac gccaggcatg ttcatcacgc agctgcgcgc
    33121 agtgtagctg ccagtcattg atatttaacg gctgctgtaa tgctggtaac agagcattta
    33181 aatcaccttg taatgccaca tgtgcctgac gcagcttgtt catttctgcc gggtcgatat
    33241 ccatatggat aacactggcg tgtggtgcga aggtgttcag tttgccggtc acccggtcat
    33301 caaaacgtgc acccacggcg atcagcaagt cgcactcctg caccgcgaag ttcgccgctt
    33361 tggtgccatg cattcccagc atgcccagat agtacggata atctgcttca actgcgccca
    33421 gccctttcag cgtgcaggtg gcaggcattt ttgtggtagc gagaaattct cgtaaagcag
    33481 gaactgcctg cgccataccc acgccaccac caacgtacag catcggtttt tgcgcttttg
    33541 ccagcatctg gcgcgcttgc tcaacttcgg catgtgggaa agtcacttcg ttttcaacgg
    33601 tggtgaacca cggttccagg tcaccgctgg ctagctggat atcttttggg atatcgacca
    33661 gaaccggacc aggacgacct gagctggcaa cgtcgaatgc ttcagccata atgcgcggca
    33721 actcttccag cgactgcacc agaaagctgt gcttggtaca ggctaacgac aatcccagga
    33781 catccacttc ctgaaatgcg tccgtgccga taaacggtgc ggacacttga ccggtgatgg
    33841 caacaacagg gatagaatct aacagtgcgt ccgcaagccc ggttatcagg ttggttgcgc
    33901 ccggaccaga cgtggcgata catacgccag ttttgccggt agcacgggca taaccgatag
    33961 ccgccattgc cgcaccctgc tcatgtcggc acagcaagtg ctccacgccg ccgtcataca
    34021 atgcatcgta aaccggcata attgcgccac ccggataacc gaaaacggtg ttcacaccct
    34081 gtgcccgcaa cgcatgtacc acccactgtg cgccattcat agttagttcc ccgtcctgaa
    34141 tcttgagaaa cagaattttg tgctgttatt cattgtctgc tcctcggtta tgtttttaag
    34201 gtcaaaaaaa acccccggac ctttcggtgc gggggtctta gttcgttaag gcttgatctc
    34261 taagcctttc ctcgtccaag tgcagccccg cacggtggga taataatcac caccacgcta
    34321 atcacgacca ggctaatcac tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt
    34381 tcgaaggaat gcctaaagag ttaccataga ttttgcgaat agcacaagat attttttggc
    34441 cgatttttat gatgcgttta ataatttcaa tagaaattgt tgttttttaa aaagaaaata
    34501 gtgagtgaaa aaaaattcaa tttcagtatg aatgttgttc ccttcctcgt agttcatcct
    34561 ttgtccgctg catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca
    34621 caaataaaca ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct
    34681 tatgcacata atagtgactc aaggagggct tatgtcactg tcaattgttc atacccgcgc
    34741 agccctggga gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc
    34801 cggcttaacg atggtgggct taccagaaac aactgtaaaa gaagctcgcg atcgcgtgcg
    34861 cagcgccatt atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaacctggc
    34921 tccagccgat ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct
    34981 ggcggcctca gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact
    35041 ggcgcttaca ggcgctctgc gtggcgttcc cggcgcaatc tccagtgcaa ctgaagctat
    35101 taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa
    35161 cggtgaagga tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa
    35221 gcacgctctc gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct
    35281 cagtgatgtt gtcggtcagg agcaaggaaa gcgaggactg gaaattaccg ccgctggcgg
    35341 gcacaacctt ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat
    35401 taatggcctg ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag
    35461 tctggtaaat gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcaccgca
    35521 tcacagtgcg tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat
    35581 ttcgttggcg cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac
    35641 actggatgcc ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc
    35701 caaaataacc tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg
    35761 acattatcag ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg
    35821 gctctcgggg ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc
    35881 cggcattttg agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt
    35941 aatggccgcc agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag
    36001 tccggaaata cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaaggaac
    36061 actgatccat ctggggttat cgattcgtgc ctggcagcgg ttattgaaag ttgcacgaac
    36121 cattgctgat attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag
    36181 ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg
    36241 catttcgccc tttttattaa tcatcagaat cggtgtagtc ttcagcacct tcaacctgcg
    36301 gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg
    36361 accacacgcg ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga
    36421 gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac
    36481 catgacgctc aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac
    36541 gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc
    36601 acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga
    36661 gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc actgtggata
    36721 cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg
    36781 aatcgctcta tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac
    36841 tgggtgtgaa ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa
    36901 aactactgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg
    36961 cgcatacctc acgacataac gagttttcta tcggtgccag cgcctcattg tgggaatgta
    37021 tgcttaatca gtggctggga cgtttgtatc aaaatcagga tgcccataca ggcttacagt
    37081 tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg
    37141 atcttcttat taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctgggat
    37201 atttcacttt agcgctttat accagtgccc cttcaaaact aaagggagat cttaattatc
    37261 tgcgacttga gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg
    37321 aagtgcccat tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta
    37381 atggttgcac ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg
    37441 tcgatagcac aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa
    37501 atacgttgat tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatatgaat
    37561 ggctggcaag gatgccggta gaaggattta cttcggagag ggttatttca gataaaaaaa
    37621 atccttagct ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca
    37681 cccggttttg gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa
    37741 cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac
    37801 taccgctcca ccgaattctt ttacaaccac cggttttatg accggctt
//
LOCUS       DAZFYC010000037        35610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFYC010000037 DAZFYC010000000
VERSION     DAZFYC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.037"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..592
                     /locus_tag="ACHKW5_002072"
                     /pseudo
     CDS             1..592
                     /locus_tag="ACHKW5_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            854..2476
                     /locus_tag="ACHKW5_002073"
                     /pseudo
     CDS             854..2476
                     /locus_tag="ACHKW5_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            2666..2779
                     /gene="yhiY"
                     /locus_tag="ACHKW5_002074"
     CDS             2666..2779
                     /gene="yhiY"
                     /locus_tag="ACHKW5_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO7511470.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2842..3909
                     /gene="yhiI"
                     /locus_tag="ACHKW5_002075"
     CDS             2842..3909
                     /gene="yhiI"
                     /locus_tag="ACHKW5_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7511471.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3906..6641
                     /gene="rbbA"
                     /locus_tag="ACHKW5_002076"
     CDS             3906..6641
                     /gene="rbbA"
                     /locus_tag="ACHKW5_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO7511472.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQGNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6641..7765
                     /gene="yhhJ"
                     /locus_tag="ACHKW5_002077"
     CDS             6641..7765
                     /gene="yhhJ"
                     /locus_tag="ACHKW5_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7511473.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7838..8113
                     /locus_tag="ACHKW5_002078"
     CDS             7838..8113
                     /locus_tag="ACHKW5_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO7511474.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            8110..8469
                     /locus_tag="ACHKW5_002079"
     CDS             8110..8469
                     /locus_tag="ACHKW5_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO7511475.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8519..9379)
                     /locus_tag="ACHKW5_002080"
     CDS             complement(8519..9379)
                     /locus_tag="ACHKW5_002080"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO7511476.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(9411..9680)
                     /locus_tag="ACHKW5_002081"
     CDS             complement(9411..9680)
                     /locus_tag="ACHKW5_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO7511477.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(9670..11178)
                     /locus_tag="ACHKW5_002082"
     CDS             complement(9670..11178)
                     /locus_tag="ACHKW5_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO7511478.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSKADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(11171..12529)
                     /locus_tag="ACHKW5_002083"
     CDS             complement(11171..12529)
                     /locus_tag="ACHKW5_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO7511479.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRITRSTV
                     YA"
     gene            complement(12606..12887)
                     /locus_tag="ACHKW5_002084"
     CDS             complement(12606..12887)
                     /locus_tag="ACHKW5_002084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7511480.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(12884..13357)
                     /locus_tag="ACHKW5_002085"
     CDS             complement(12884..13357)
                     /locus_tag="ACHKW5_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7511481.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(13382..14128)
                     /locus_tag="ACHKW5_002086"
     CDS             complement(13382..14128)
                     /locus_tag="ACHKW5_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7511482.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(14327..14728)
                     /gene="nikR"
                     /locus_tag="ACHKW5_002087"
     CDS             complement(14327..14728)
                     /gene="nikR"
                     /locus_tag="ACHKW5_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO7511483.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(14734..15540)
                     /gene="nikE"
                     /locus_tag="ACHKW5_002088"
     CDS             complement(14734..15540)
                     /gene="nikE"
                     /locus_tag="ACHKW5_002088"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO7511484.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(15537..16301)
                     /gene="nikD"
                     /locus_tag="ACHKW5_002089"
     CDS             complement(15537..16301)
                     /gene="nikD"
                     /locus_tag="ACHKW5_002089"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO7511485.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(16301..17134)
                     /gene="nikC"
                     /locus_tag="ACHKW5_002090"
     CDS             complement(16301..17134)
                     /gene="nikC"
                     /locus_tag="ACHKW5_002090"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO7511486.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(17131..18075)
                     /gene="nikB"
                     /locus_tag="ACHKW5_002091"
     CDS             complement(17131..18075)
                     /gene="nikB"
                     /locus_tag="ACHKW5_002091"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO7511487.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(18075..19649)
                     /gene="nikA"
                     /locus_tag="ACHKW5_002092"
     CDS             complement(18075..19649)
                     /gene="nikA"
                     /locus_tag="ACHKW5_002092"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7511488.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(19760..20347)
                     /gene="acpT"
                     /locus_tag="ACHKW5_002093"
     CDS             complement(19760..20347)
                     /gene="acpT"
                     /locus_tag="ACHKW5_002093"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO7511489.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(20402..21451)
                     /gene="yhhT"
                     /locus_tag="ACHKW5_002094"
     CDS             complement(20402..21451)
                     /gene="yhhT"
                     /locus_tag="ACHKW5_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7511490.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            21583..22800
                     /gene="yhhS"
                     /locus_tag="ACHKW5_002095"
     CDS             21583..22800
                     /gene="yhhS"
                     /locus_tag="ACHKW5_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7511491.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(22804..23361)
                     /gene="dcrB"
                     /locus_tag="ACHKW5_002096"
     CDS             complement(22804..23361)
                     /gene="dcrB"
                     /locus_tag="ACHKW5_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO7511492.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(23434..24099)
                     /gene="yhhQ"
                     /locus_tag="ACHKW5_002097"
     CDS             complement(23434..24099)
                     /gene="yhhQ"
                     /locus_tag="ACHKW5_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO7511493.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            24320..24565
                     /gene="tusA"
                     /locus_tag="ACHKW5_002098"
     CDS             24320..24565
                     /gene="tusA"
                     /locus_tag="ACHKW5_002098"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO7511494.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(24667..26865)
                     /gene="zntA"
                     /locus_tag="ACHKW5_002099"
     CDS             complement(24667..26865)
                     /gene="zntA"
                     /locus_tag="ACHKW5_002099"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO7511495.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(26939..27565)
                     /gene="yhhN"
                     /locus_tag="ACHKW5_002100"
     CDS             complement(26939..27565)
                     /gene="yhhN"
                     /locus_tag="ACHKW5_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO7511496.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            27706..28065
                     /gene="yhhM"
                     /locus_tag="ACHKW5_002101"
     CDS             27706..28065
                     /gene="yhhM"
                     /locus_tag="ACHKW5_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO7511497.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(28068..28337)
                     /gene="yhhL"
                     /locus_tag="ACHKW5_002102"
     CDS             complement(28068..28337)
                     /gene="yhhL"
                     /locus_tag="ACHKW5_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO7511498.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(28327..28923)
                     /gene="rsmD"
                     /locus_tag="ACHKW5_002103"
     CDS             complement(28327..28923)
                     /gene="rsmD"
                     /locus_tag="ACHKW5_002103"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO7511499.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            29073..30566
                     /gene="ftsY"
                     /locus_tag="ACHKW5_002104"
     CDS             29073..30566
                     /gene="ftsY"
                     /locus_tag="ACHKW5_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO7511500.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            30569..31237
                     /gene="ftsE"
                     /locus_tag="ACHKW5_002105"
     CDS             30569..31237
                     /gene="ftsE"
                     /locus_tag="ACHKW5_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO7511501.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            31230..32288
                     /gene="ftsX"
                     /locus_tag="ACHKW5_002106"
     CDS             31230..32288
                     /gene="ftsX"
                     /locus_tag="ACHKW5_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO7511502.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            32533..33387
                     /gene="rpoH"
                     /locus_tag="ACHKW5_002107"
     CDS             32533..33387
                     /gene="rpoH"
                     /locus_tag="ACHKW5_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO7511503.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            33658..34761
                     /gene="livJ"
                     /locus_tag="ACHKW5_002108"
     CDS             33658..34761
                     /gene="livJ"
                     /locus_tag="ACHKW5_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO7511504.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(34960..35343)
                     /gene="panM"
                     /locus_tag="ACHKW5_002109"
     CDS             complement(34960..35343)
                     /gene="panM"
                     /locus_tag="ACHKW5_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO7511505.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         8713 a         9044 c         9513 g         8340 t
ORIGIN      
        1 tcgttctggc tagaaatcat gaaatcaatt ttaatggtaa ctatactgca tgactagaaa
       61 attataaact ctctgccggt gatacgtata acttaattaa aacgctcgat gtttgtcatt
      121 atgactcaga aaggaaagtt ttaaccagtc atcacggaat aataagagat gaagaaaaaa
      181 atgttattgt ctcggtgctt tgcaggttcc atttaatcag atgaaaaatc ctactgatcc
      241 tgaggaatta gccaatattt ttaataaaaa acataaagaa cacatggatg atcctctatt
      301 ccatctaatc cgaagcaata ctttgaaacc cacacctgta tatgctaatt attttgataa
      361 cactactgat ttcagaccag cacgtaaacg tatcttcata tatggggaga cactcaaagg
      421 tgaagacccc agcaaataca ttagacaaaa atatgggcat catggggtcg gggtagatca
      481 gaatcaacaa tttgataatg gtattatggg gctaaattcc ctgaaggaag cacgtgataa
      541 aaataataaa ataatttatt cctcagggct tagttgtttt caatctcatt aaaactccat
      601 gaatacggtg gtatgtcttt ttaatcctgc cagtatttca taggttttct taatcgattg
      661 tagaggcatg ttctgatgac gcctctacaa tctttaggga ctttctctgc tatgagataa
      721 gaaatttcat attatgcttt aaggctgaat gatttgtgca aaatataatt atttttccat
      781 cgaacatgag gtgtatgtca taagattaaa tgttaactat gcattcctcg cggtgaatta
      841 tatgaggtaa aagatgaaaa ttggaactgt ggcaggcact aacgacagta caacgacaat
      901 cgcaacaaat gatatggtgc aagagcatgt tactaacttc actaaagaac tgtttgggta
      961 tattgcaaat ggtataggtg atgatatatc aagtattgcc aggacaatgc tcggtgaagt
     1021 tgtggaaaaa attgatgatt ggcaaataga acgtttccaa caatctattc gagatgacaa
     1081 aatatcattt accatacaaa cgaatcattc agaaaaatat agtatgctat cggacatgcg
     1141 cgctcatatt ctacgtaggg ataataatta tcagtttatt gtgacaatta atagtaaaaa
     1201 ttacggctgc tatcttgata ataccgatat taattggtgc agcattgttt atttacttaa
     1261 taatatgaca gtaaatgatg atgctaatga tgttgcagtt actgagtcgt acaaacccgt
     1321 ctgggactgg aaaatatcgc aattcaatgt atctgatatt aaattcgaaa caatgataaa
     1381 cccccagttc gctgacagaa cctatttctc caattgttca cctgttgatc ccacaagtac
     1441 cagaccaacc tattttggcg ataccgatgg ctcagtcggt gcagttttgt acgcgctttt
     1501 tgcaacggga catttgagaa tcatggctga aggagaaaat ttccttagcc agttgcttaa
     1561 tatagaagat gaagttctca acgtattgct aagggagaac tttaatgagc aacttgacac
     1621 taatgttaat acaataatca gtattctgaa cagaagagat atagttttag aaagtcttca
     1681 gccttattta gttattaata aagacgctgt gacgccatgt acgtttcttg gcgaccaaac
     1741 cggcgatcga tttagtaata tctgtggcga tcaatttatc atcgatttgt taaaacgcat
     1801 aatgagtatc aatgaaaatg ttcatgtatt agctggaaat catgaaacga attgcaatgg
     1861 taactatatg cagaacttca cgcgcatgaa accacttgat gaggatacct acgctgggat
     1921 aaaagattat cctgtatgtt tctatggccc caaatacaaa ataatggcaa atcatcatgg
     1981 gataactttt gatgatcagc gaaaacgtta cattatagga ccgataacag tctctagtga
     2041 tgaaatgacc aatgctctcg atccggtcga attagcggca attatcaata aaaagcacca
     2101 tgctgtaatt aatggcaaaa agtttaaaaa tagccgggct atctcttgtc gatcctttaa
     2161 tcgttatttt tctgtttcga cagattacag acctaaactt gaagcactat tagcatgctc
     2221 acaaatgtta ggtataaatc aagttgttgc acataacggt aatggtggaa gagaatgtat
     2281 aggtgaaacg gggacagttt taggtcttaa cgcccgtgac agtaaacatg ctggaagaat
     2341 gttcagcatg cataattgcc aaattaaccc tggtgcaggg cctgagataa ccactccctg
     2401 gaagtcttag cagcatgaaa aaaacagaaa tggacttatg cctttgatta gaaggcgcac
     2461 aatgttacag ctctgaacca tagacaacat gaatattaat gttactatct ggttgagact
     2521 gttgataaaa cgtaaaaagg aatgctctgt tcctgcaact gaacaggcgt gaaacgcgat
     2581 ccaacaggat ggataccgtt ttgtcagcca gttttataga tcacacttat gagtatgcgt
     2641 gtttcattta cggccaggaa tgctgatgat gacaacatta ctacccgttt tcactaagcc
     2701 ttccccgttg gcgctcaatg ctctgcgcgc tggtcgtatt tgtcgtttcc ttcttatccc
     2761 ggatggaaga atccgctgaa cgtcgttcgg cggcagttgt aacacgcgtc gtttgcttac
     2821 agggattatg gcgggccgat tatggataag agtaagcgcc atctggcgtg gtgggttgtc
     2881 ggggcactgg cggtggctgc cgtcgtggcg tggtggctgt tgcgcccggc gggtgtgccg
     2941 gaaggctttg ctgtcagcaa tgggcgcatt gaagcgacgg aagtggatat cgccagcaaa
     3001 attgccgggc gtatcgacac cattctggtg aaagaaggcc agtttgttcg cgaaggtgaa
     3061 gtgctggcga agatggatac tcgcgtgttg caggaacagc gactggaagc catcgcgcaa
     3121 atcaaagagg cacaaagcgc cgttgccgcc gcgcaggctt tgctggagca acgacaaagc
     3181 gaaactcgcg ccgcgcagtc gctggttaat caacgccagg cagaactgga ctccgtagca
     3241 aaacgtcata tgcgttcccg ttcactggcc caacgagggg ctatttctgc gcaacagctg
     3301 gatgacgacc gcgccgccgc tgagagcgcc cgagctgcgc tggaatcggc aaaagctcag
     3361 gtttctgctt ctaaagccgc tatagaggcg gcacgtacca atatcattca ggcgcaaacc
     3421 cgcgtcgaag cggcacaagc cactgaacgg cgcattgccg cagatatcga tgacagcgaa
     3481 ctgaaagccc cgcgtgacgg acgcgtgcag tatcgggttg ccgagccagg ggaagtgctg
     3541 gcggcaggcg gtcgggtgct gaatatggtc gatctcagcg acgtctatat gaccttcttc
     3601 ctgccaaccg aacaggcggg cacgctgaaa ctgggtggtg aagcccggct gatcctcgat
     3661 gccgcgccag atctgcgtat tccggcgacc atcagttttg tcgccagtgt cgcccagttc
     3721 acgccaaaaa ccgtcgaaac cagcgatgaa cggctgaaac tgatgttccg cgtcaaagcg
     3781 cgtatcccac cggaattact ccagcagcat ctggaatatg tcaaaaccgg tttaccgggc
     3841 gtagcgtggg tgcgggtgaa tgaagaactt ccgtggcctg acgacctcgt ggtgaggttg
     3901 ccgcaatgac gcatctggaa ctggttcccg tcccgcctgt cgcgcaactg gcgggcgtga
     3961 gccagcatta tggaaaaacc gttgcgctga acaatattac tctcgatatt ccggcccgct
     4021 gtatggtcgg gctgattggt ccggatggcg tcgggaagtc gagcttattg tcgttgattt
     4081 ccggtgcccg cgtcattgag cagggcaatg tgatggtgct gggcggcgat atgcgcgacc
     4141 cgaggcatcg ccgcgacgtc tgcccgcgca tcgcctggat gccgcagggg ctgggcaaaa
     4201 acctctacca caccttgtcg gtgtatgaaa acgtcgattt tttcgctcgc ctgttcggtc
     4261 acgacaaagc ggagcgggaa gtgcgaatta atgagctgct gaccagcacg gggttagcac
     4321 cgtttcgcga tcgtccggca gggaaactct ccggcgggat gaagcaaaaa cttgggctgt
     4381 gctgcgcgtt aatccacgac ccggaactgt tgatccttga tgagccaaca acgggggttg
     4441 acccgctctc ccgcgcccag ttctgggatc tgatcgacag tattcgtcag cggcagagca
     4501 atatgagcgt gctggtcgcc accgcctata tggaagaggc cgaacgcttc gactggctgg
     4561 tagcgatgaa tgccggagaa gtgctggcaa ctggcagcgc cgaagagcta cggcagcaaa
     4621 cgcaaagcgc tacgctggaa gaagcattta taaatctgtt accgcaagcg caacgccagg
     4681 cgcatcaggc ggtagtgatc ccaccgtatc aacctgaaaa cgcagagatt gccatcgaag
     4741 cgcgcgatct gaccatgcgt tttggttcct tcgttgccgt tgatcacgtt aatttccgca
     4801 ttccacgcgg ggagattttt ggattccttg gttcgaacgg ctgcggtaaa tccaccacca
     4861 tgaaaatgct caccggactg ctgcccgcca gcgaaggtga ggcgtggctg ttcgggcaac
     4921 cggttgatcc aaaagatatc gatacccgcc gtcgggtggg ctatatgtcg caggcgtttt
     4981 cgctctataa cgaactcacc gtgcggcaaa accttgagtt acatgcccgt ttgtttcaca
     5041 tcccggaagc ggaaattccc gcaagagtgg ctgaaatgag cgagcgtttt aagctcaacg
     5101 acgttgaaga tattctgccg gagtcattgc cgctcggcat tcgccagcgg ctttcgctgg
     5161 cggtggcggt gattcatcgc ccggagatgt taatcctcga tgagcctact tctggtgtcg
     5221 atccggtggc gagggatatg ttctggcagt tgatggtcga tctctcgcgc caggacaaag
     5281 tgactatctt catctccacc cacttcatga acgaagcgga acgttgcgac cgcatctcac
     5341 tgatgcacgc cggaaaagtg cttgccagcg gtacaccgca ggaactggtt gagaaacgcg
     5401 gagccgccag tctggaagag gcatttatcg cctatttgca ggaagcggca gggcagggca
     5461 acgaagccga agcgccgccc gtggtacacg acaccaccca cgcgccgcgt cagggattta
     5521 gcctgcgccg tctgtttagc tacagccgcc gcgaagcgct ggaactgcga cgcgatccag
     5581 tacgttcgac gctggcgctg atgggaacgg tgatcctgat gctgataatg ggttacggca
     5641 tcagtatgga tgtggaaaac ctgcgctttg ctgtgctcga ccgcgaccag accgtcagta
     5701 gccaggcgtg gacgctcaat ctctccggtt cccgttactt tatcgaacag ccgccgctca
     5761 ccagttatga cgagcttgat cgtcggatgc gtgcgggcga tatcacggtg gcgattgaga
     5821 tcccgcccaa tttcgggcgc gatatcgcgc gtggtacgcc tgtggaactc ggcgtctgga
     5881 tcgacggagc gatgcccagt cgtgccgaaa cggtaaaagg ttacgtgcag gcaatgcacc
     5941 agagttggct tcaggatgtg gcgagccgac aatcgacacc cgccagccaa agcgggctga
     6001 tgaacattga gacgcgttat cgctataacc cggacgtaaa aagcctgcca gcgattgttc
     6061 cggcggtgat cccgcttctg ctgatgatga tcccgtcaat gctaagcgcc cttagcgtgg
     6121 tgcgggaaaa agagctaggg tcgattatca acctttacgt gacccccacc acccgtagcg
     6181 aatttttgct tggcaaacag ctgccgtaca tcgcgctggg gatgctgaac tttttcctgc
     6241 tctgcggcct gtcggtgttt gtgtttggcg taccgcataa aggcagtttc ctgacgctca
     6301 ccctggcggc gctgctgtat atcatcattg ccaccggaat ggggctgctg atctccacct
     6361 ttatgaaaag ccagattgcc gccattttcg gaacggcgat tatcacattg atcccggcga
     6421 cacagttttc cgggatgatc gatccggtag cttcgctgga agggcctgga cgttggatcg
     6481 gcgaggttta cccgaccagt cattttctga ctatcgcccg cgggacgttc tcgaaggcgc
     6541 tggatctgac tgatttgtgg caacttttta tcccgttgct gatagccatc ccgctggtga
     6601 tgggcttaag tatcctgctg ctgaaaaaac aggagggatg atgcgccatt tacgcaatat
     6661 ttttaatctg ggtatcaaag agttgcgcag tctgctcggt gataaagcga tgctgacgct
     6721 gattgtcttc tcgtttacgg tgtcggtgta ttcgtcagcg accgttacgc ctggatcgtt
     6781 gaacctcgcg ccgatcgcca ttgccgatat ggatcaatcg cagttatcga accggatcgt
     6841 taacagcttc tatcgcccgt ggtttttgcc accggagatg atcaccgccg atgagatgga
     6901 tgccggactg gacgccggac gctatacctt cgcgataaat attccgccta attttcagcg
     6961 tgatgtcctc gccggacgcc agccggatat tcaggtgaac gtcgatgcca cgcgcatgag
     7021 ccaggcattt accggcaatg ggtatatcca gaatattatc aacggtgaag tgaacagctt
     7081 tgtcgcgcgc taccgtgata acagcgaacc gttggtatcg ctggaaaccc ggatgcgctt
     7141 taacccgaac cttgatcccg cgtggtttgg cggggtgatg gcgatcatca acaacattac
     7201 catgctggcg attgtattga ccggatcggc gctgatccgc gagcgtgaac acggcacggt
     7261 ggaacattta ctggtgatgc cgataacgcc gtttgagatc atgatggcga agatctggtc
     7321 gatggggctg gtggtgctgg tggtatcggg attatcgctg gtgctgatgg tgaaaggtgt
     7381 actgggcgta ccgattgaag gctcgatccc gctgtttatg ctgggcgtgg cgctcagtct
     7441 gtttgccacc acgtcaatcg gcatttttat ggggacgata gcgcgttcaa tgccgcaact
     7501 ggggctgctg gtgattctgg tgctgctacc gttgcaaatg ctttctggtg gatccacgcc
     7561 gcgcgaaagt atgccgcaga tggtgcagga cattatgctg accatgccga cgacacactt
     7621 tgttagcctc gcgcaggcca tcctctaccg gggtgccgga ttcgaaatcg tctggccgca
     7681 gttcctgacg ctgatggcaa ttggcggcgc gtttttcacc atcgcactgc tgcgattcag
     7741 gaagacgatt gggacaatgg cgtaaggaag ggaaacttga cttcatactg tgatgtcata
     7801 ctattgatgt cataaagtga agtcataagg acatgccatg agaaccaagg tacaggcttt
     7861 acggaagaaa caaaaaaata cattggatca aatatttaaa actcctgttc ctcaagggat
     7921 caagtggtct gatatagagt cactggttaa agcattaggc ggagaaatta aggaaggaag
     7981 aggttcgcgt tgtaagttca tactaaatat gagcgttgcg tgtttccatc ggcctcatcc
     8041 gtcgccagat accgataaag gcgctgtaga aagcgtgcgt gactggttac taagtatagg
     8101 ggtaaaacca tgatcaaact gaagacgcca aactctatgg agattgccgg gcaacctgca
     8161 gttatcactt acgtgccaga actgaatgca tttcgtggca aattcctggg cttgtcaggt
     8221 tattgtgatt ttgtctctga cagcattcag gggctgcaaa aagaagggga actttccctg
     8281 ctagagtatc ttgaggattg taaagctgcg ggtattgagc cgtatgcgcg tacagaaaag
     8341 attaagacct ttacgttacg ctatccagaa tcgctgagtg aacgccttaa taatgcggct
     8401 gcgcagcaac aagtctctgt taatacctat attattgaaa cactgaatga gcgtttgaat
     8461 catttataac ttatagcggc ccaacaattt ctgttgcgcc gctatatttt tgtggctctt
     8521 aagatgcctt tctaaccaac cgatgcccct cattcatcat caggattttg ctatacacat
     8581 cttcctcgac ggcgtttttt gctgatgcca tgactcgtgc cagattcgcc tcgttgtgat
     8641 tattcacata cgctttgccg acggcggtga taaacgcttt gcgcagatcg ctggcgatat
     8701 ttactttagc gacgcgataa ttaacaaagc tgcgtaatat ctccggggcg ataccggagc
     8761 cgccgtgaat aaccaacggc accggacaga cttcggcaat gcgcttcagc agggggatat
     8821 cgatacgcgg tatatcgtcc aggccatgaa cgttgccaat agaaactgcc agcatgtcgc
     8881 aaccggtacg ctcgacaaag gtcttcacct tttccggttc cgttttgcaa tcggcttcgc
     8941 tgacatgatc atcttctttg ccgagaatcg cccccagctc cgcttcaacc ggtacaccat
     9001 aggatttaca gaaatcgaca gcttcctggg tgaaagcgat attctcttca aaagagaatg
     9061 cggcaccgtc aatcatgacc gaggtaaagc ccgcgcgcac cgcctgtttc acatcctcca
     9121 gagttttccc gtgatccaga tgaagactga ccggaacatc cattttatcg gcgtgacgtt
     9181 tcacgatttc ataaatgtaa tcgtatcccg agagttgtgc attggttggc gcaatctgaa
     9241 tgaagttggg taatcccgaa cgctcaatag cgtttaaaat tgaaattgtc gtctccagat
     9301 tggtggtatt aaatgcgccc gctaataccc gtttttcctg aatgcggtcg agcagtatcc
     9361 gtccattaac taatggcata ttgaaaacct catcttttaa tatcagttaa ttattcagct
     9421 tcaccgaagc ggtcgcgaaa taagtcggtg agagtctgta tggcctcgtt ttcatcatca
     9481 ccgtcgcagc gtatttccac cgtttttccg ccttttattc cggcgcgcat aatattcatc
     9541 atgctggtgg cattaatatc attaccctca tagtgcagcg tcactttaca gctatatttt
     9601 ttggcggctt tcgccagaca ggccgccgga cgtgcatgca ggccagtact gtttctgacc
     9661 tccacggttt tattcaacat agtgttcctc ccgatgatgt aatggcatca cgccttcttg
     9721 ttgtaatttc aactgatacc agcttttcca tttgtgctga taacgctgta attgatggta
     9781 tctcgcccga ttcggtaaca aacgttgggc tatagcgact ggcggcggtg gaatatccgg
     9841 tgcggcggtt aataatgctc cgacagaaac cgcctcgtta aatgcaattg cttcaaccgg
     9901 acgctgtaaa atatccgcct ttaattgcag ccaaagagga ttatgggttg ccggaccgat
     9961 cacttttaac gcgctggtgc caggcacgtt aaaacaggcg aagcagttgg caaattccat
    10021 ggttaatccc agtgatacgc tggcaattaa catgtcgata cttaacgtat cgccaagccc
    10081 ataaaataat ccacgggtat ggcgattttt atagggcgaa cccgaaccgc gaagatgtgg
    10141 aataaagacg ggaatgtcat cgagcaacca attccccgcc agataatcag catgtccgcg
    10201 agtaagtgat gcggcgatct cctcatcggt tagccggaac gtgttgcgca gccactccag
    10261 cgcgaaaccg cccacgggca gcgacgcaaa cagggtgaac tggttggcaa gtgagtaaca
    10321 gccgtttgcc agcttgtcac gtttggcctg ttcatccagc gtcggttgtg tatccagttg
    10381 cagaatgcct tccgtggtcc ccgtcgagtt aaggatatcg cctggcatca tctgcaacgc
    10441 tcgcgcaccc accatatggt catgtccggc cagcgtcacg ctgaccggtt gcgagaaccc
    10501 aagcgtcttg caaagtgact cgctgaccca tccggcgtgc tcgcctggct gcaccaatgg
    10561 cgcaaatgcc gtacagggaa cgtgtaacaa cgccgccgct ttcgctgacc attcgccttt
    10621 gcgcacgtcc agacacagcg tgcggctggc taaggtgata tccgtgcgct gttcgccagt
    10681 cagccgccag agcagcactt ccggcgcatg tagccagcgc aggccgcggc gattacgcag
    10741 cgggtaatgt tccagcaacc agcgcatttt gaaggcagag taattgctgt gtagtggtag
    10801 accgcagatg tcataaagtg ctgcactgtc tgccttgcta agcgttgcca gatactcttc
    10861 accgcgacgg tcataccatg ccagcattgg cgtcaggatc tcgccatgct cgtcaaggaa
    10921 cacgcctgat tcgccaaaac tggcaatgct gatccgtctg actggcagcg gcgaagcggc
    10981 gttcagttgc gctatcgcct ggcggacctc ctgccagagg gcgtcgatat cgaaatcgac
    11041 atcgccctgt ggggagatct gctttgccgt cacgaattta tgcgcgccca acgtcgttgc
    11101 gtccaggcag gaaaagcagg tgactttgca attggtggtg ccaatatcga taacgatagc
    11161 tgcgctgtta tcaggcataa accgtgctcc gcgttattcg gaaaatgcca tacagtacca
    11221 gggcgataac cggcaccaga ccaatggcga tgatattgcc gttgaagatt tccagcagcc
    11281 agaagcggaa cgggttaccg ccatcaagga aactggaaac ctggccgctg gagccttcgg
    11341 caaatttgaa gttaacgtct ttcgccatgc cggtaataaa cggtgcgatt ttagtcgcaa
    11401 tccacaggtc ggcaataatc accgggatcg ccgccagaac ggtgcggaaa atattgccac
    11461 ggctggctaa agcaatcatc gacgccatta ccgccaggtt ggcaaggtcg ccaagcggca
    11521 ggacttcatt acccggaagg acaaacgcga taaagaccga gataggcgtc agcagcaggg
    11581 cggtggagat aatcgccggg ttacctacgg caacggcgat atccaggccg atatagagat
    11641 cgtcacggtc agggtatttg gcattgagat actttttaat ggcttcagac agcggtaata
    11701 aaccttccat caggatgcgt accatgcgtg gcaggatgaa catcacaccg ccaatgctga
    11761 tgccaagcag caacactttt ttgaaatcgt atcctgcaat cacgccgagc agtatgccca
    11821 gaatagtgcc aaccatcatc ggttcgccga agatgccaaa ctttttctgt acggtttccg
    11881 ggtcgatatg aatacgattg aggccgggga tatggtcgat gattttgtcg accagcagac
    11941 cgaccgggaa gaacaccacc gaagagagcg tcggcaatga gatcccttcc agtccaaagt
    12001 atttttgtac cagtggcgca gaccagtcgg cgagtttaag tactaccacc gcagcaatcg
    12061 ccgccgccag caaaccaaag aacaggctgc cggtgctgta ataaaccaac gtaccagcaa
    12121 gggcaaaatg ccagtagttc cacatatcta cgtcaacggt acgggtttta tttaacgcca
    12181 gcatcgccac gttaatcaga atggtcaggg ggataacaaa cggggcaatt ggcgaacccc
    12241 atgtaatagc ggaaagcggc ggccagccta aatctaccac cggtaaatta attccggtgc
    12301 gttcaaccat cgcctgggcc gctggcccga cattgctggt cagaatatcg aaaatggcat
    12361 aaataccgac aaaaccaatg ccgactgtta atgacgaacg taaggcttta gcgggtttta
    12421 ctttaaaaca gagagcaaga ataaataaca ccaacggcaa cattaccgtc gggccaaatc
    12481 ccaatatata ttgcacaata ttatagaggg tatgcgcgat atcgttcata ataaacctct
    12541 ttttcaggcg agagcgcacc tcgcgttagc gcctgatgga gtgggttgaa attaattatt
    12601 attaattaat ttttaaggat cgaaacaatt tgcgccagta ctttctcttc gccaataccg
    12661 gtaataatcg gcaagccgtt aactaccggg acgtcgagtt cataaggcac tttagtcgtt
    12721 gagactacca gtgctacgcc atcagtattt ccaggtaaag aggcaacgtt ggtttgggaa
    12781 taattgacgt taagaccgtg ctctttacaa tattccatga ctttttctgt taccgccgtg
    12841 gatgtggcaa tacccgtggc gcagacaaat aaaatggttt gactcatatt tcacctcgtt
    12901 ctttcatttt gtttaagatt actgtgtgta tttctgttgt ttccatcgca agcagggctt
    12961 gcataaaggg cgtatcctga atcatttcca taatccagcc cagggcattt aattgtttat
    13021 tactttcgct taaggcgagc aaaaaaatca ccctgacagg tacggggtca ggatcgccgc
    13081 ccatatcggc aaactctacc ggttccggca ggatgccgac ggcaattgca ttgtgcctga
    13141 catgtttatg gtccgtgtgc ggaatagcga cgcacggtgg ttcggtaggc agccccgttg
    13201 ggtacgtcgc ctcacgtttc agtacgtgat cggcaaaatc agggacgacc agctcggcgg
    13261 caaccaacgg ttgcgctaac tgacggatga tctgttcaac gcttaaccct gccggaagtc
    13321 gttgaatata attttcctga aaatgtatgc cttgcatttt actcctctta aaaaagtgta
    13381 cttaactcac ttcccgccgt tgcagtacgg tgccaagata atatttattt ccttttaacc
    13441 agacgttgcc aaactcaatg ggtaatcctc gggagaaaaa gactaactgc tccagatgca
    13501 aaacgggtgc gtcttcactg atatctaaaa aatgaccacg ttcattacca attaatcgcg
    13561 cggcatagcg actttccgag taacgaattt ttctttgcga caaactttct attgttggaa
    13621 ataaattgtg ttgattaaaa tcgatttccg caataccggg gcatagctca atattgatac
    13681 ggttctctat cagcatcgct ttttcatcac caattgagcg taagcgttca aggtaaagaa
    13741 tatcctgacc gggcgttatt cttaatttct ctgccacata acgattagcc gattcaatcc
    13801 gtgacgtaat aacttcagtg gtaaaatgta ttttctggct ttccagcgat tccgcgaatg
    13861 acaataatcc ttcacctaac ggatatgcca cgttctcttt tttgacaaat gttcccttac
    13921 cctggacctg tattaacacg ccttctttta ccagttgggc gaccgctttt ttaacggttc
    13981 cccgactcac ttccagcata tccataatct ggtattccga aggaatgcga tcgtcttcca
    14041 ccagatctcc ggtataaata ctttctctta tccactccac cacttgttga tagagcggta
    14101 tttgtgacga cttacttaat gatttcatgg cgcgcacctg aacatttaca gttgtctatg
    14161 gacaatcatg gacaacttca agcgtatagt aggtgaacgg gagaaaaaga ggatcgaagc
    14221 gtctcacatt ttgagggacc aaatcgtgat caaaaaaatg tgacgtgagt cgatatttgt
    14281 cagaagttta ctgcgccgta aggaaataac ggcgcgttgg tcggtgttaa tcttccttcg
    14341 gcaagcactg caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat
    14401 gctgcacgtc acccatgtca cctttcaaca cggcgatttc cagacagtcg tcgtggttga
    14461 tgtgcacatg cagcgtggcg acggagaggt cgtggtgatg atgctgggtg gaaacaatac
    14521 ggctggctaa gtcgcgtttt tcgtgttcat acacatacga cagcaccgcg aaaccttgtg
    14581 tgccgtgctg ctgggtagct tcttgcgcca gggcgctacg cagaatatcg cggatagctt
    14641 cggaacggtt gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt
    14701 catcaagcgt gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg
    14761 cacggggaat gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt
    14821 taatttatct cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg
    14881 ctgacagaag cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa
    14941 ctgttgttgt agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt
    15001 agaaacggct tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca
    15061 gacgcgctgg agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc
    15121 gagatcgacc gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga
    15181 tagcaggtgg cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc
    15241 gctgatggag tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg
    15301 attgagtttc gccagcggtt cgccgcgcca gctaatattc ccttgtgagg gagactctaa
    15361 gcctaccagc agccgcgcga gggtgctttt tccgcagccg ctgcgtccca gcagggcgac
    15421 ggtttcgccg cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt
    15481 tccgctaaat ccaccgtggg catagtgatg ggaaaggccg cagacgttaa gtaaggtcat
    15541 gatgccagct ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcaccgta
    15601 tgtttggggg cgttaaacag cgtttctaca tcgccctgtt cgacaatttt accttgtgac
    15661 atcaccgcca cgtcatccgc cagacgcgcc acaacgccca tatcatgggt gaccagcagc
    15721 attcccggcg cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct
    15781 accacgtcga ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc
    15841 gccatcgcaa tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc
    15901 agcacgcgcg cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca
    15961 tcggcgggtt tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt
    16021 ggattaaagg cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg
    16081 caaggcgaaa ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg
    16141 ggcaaaatgc ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact
    16201 aacgccagca cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg
    16261 gcctgtagcg cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca
    16321 ccagatgagg gtccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc
    16381 tgataaacag cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc
    16441 gcgcgtcgtt aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga
    16501 aagacatccc cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca
    16561 gcgaagggat caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcgccag
    16621 aaagccgtga cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca
    16681 tgcgtgcata ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg
    16741 tgccgagcac gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat
    16801 cggcgacgcg catggtggcc tgatcaacgc gcccgccaat caacccggcg ctgccgccaa
    16861 taaccagccc taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc
    16921 gggtcgctgc catcagccgc gagaaaatat cgcgacctaa gtgatcggtg cccagccagt
    16981 gctgcgcatc cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg tcatacggca
    17041 gccaccactg gctggtgagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc
    17101 gtaccgacca gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat
    17161 gcgcgggtcc agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac
    17221 caccatcatc agcgtaaagc actggatcac cggatagtca cggttaaaaa tcgccgacac
    17281 cgcatagcgc ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccaat
    17341 cagttcgccg atgtgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcaggat
    17401 gtggcgacgt tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg
    17461 ctgaccggcg acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat
    17521 aaaggcaatg gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg
    17581 tagccattgc agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg
    17641 catcgacacg ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg
    17701 gcgcgccgcc cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc
    17761 tgcaagttcc agcgtggcgg gcaggaagtt cagcatatcg tccagcaccg ggcgttggct
    17821 ggcgaatgag ataccaaagt caagatgcag cgccttccac aaccaggtgc cgtactggac
    17881 gtacagcggc tgatccagcc cgagcatggt gcgggtagag gccagcatct ccggcgtcgg
    17941 cggcaggtta gataaacgca gataatcgag cgccgggtcg ccggtaccga ggcgcagcat
    18001 tagaaaaatg atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa
    18061 tacgtaacgc aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcagtggcg
    18121 atcggcgcgt aggggatgtt acccagctcc ggttttgata ccaccatcat tgagatgtaa
    18181 ctgataggca gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc
    18241 tgacgttgcg tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggt
    18301 ttgtcggcta atccttgctg tgcctggaag tcagcgtgtg acggtacgcg cattgaactg
    18361 aggaaggcgt gtggatcgta tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga
    18421 ccgtcgcgct gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg
    18481 ccaatctggc gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg
    18541 ccgatgaacg acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc
    18601 gcaggcagcg tccaaccggc tttttccagc aacgctttcg ctttctgcgg atcgtactgg
    18661 cgcggtttca ggccgaggtt ggcgtagggc acagaagggg caaacagggt gtcggcgacc
    18721 tgctgggtgc catacaacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga
    18781 gcttcacgta ctgccagctc attggtgggg gctttggcgg tattgagcgc cagcatcacg
    18841 gtttcgatcg gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag
    18901 gtatcgagcg gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac
    18961 gccaccgcgc gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc
    19021 ttttcgcccc agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc
    19081 tgcaaaatcc atggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt
    19141 ttaaactgcg agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa
    19201 ggatagtagg cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca
    19261 acaatctggt ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg
    19321 aagttttctg ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca
    19381 cgcagggtga aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac
    19441 gggatcaccg aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg
    19501 aacatctggt taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg
    19561 gtagtgattt catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca
    19621 aatagagtgc ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg
    19681 aaataagtat gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat
    19741 gggcagtcgg gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga
    19801 gcgtaaaggg ggtgggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga
    19861 ctgacagcga ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac
    19921 gctgtttaac gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat
    19981 caggatgcac ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc
    20041 agcgccagtt ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac
    20101 tcaacagcag agcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg
    20161 gcgcgaatgc aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt
    20221 gcgaaagcaa tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct
    20281 cgcgtaaacc cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc
    20341 gatacatatc agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc
    20401 ctcatcccgg taaccgactt ttcggtctgc ccggccccag taaaatcgcc agtttgctgc
    20461 cacctttcgt cgtttccatc cagattttac acacgctggt taatggcacc gaaagtagca
    20521 tccctaccgg gccgagcagc catccccaga ttaacaatga aagaaatacc accatggtgg
    20581 acatccccag gcgatggccc atcatccgtg gctctaaaat attgccgatg accatatgga
    20641 caactaaaaa caatgcgccg accagaatac attcgtaaat accattaaac agcagcacct
    20701 gaatcattgg cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttaagcaaaa
    20761 acgccagtac tgcccacatc agcgcaaact gtacccccat cagctccagc cccagccaga
    20821 cgatgacacc tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt
    20881 taagtgcgcg gtgtaatccc gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt
    20941 tgtaagggac gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca
    21001 cgctcgccat tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt
    21061 ccgagtccat ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca
    21121 tctcctgcaa tttaaaaagt ttgcgcgtca actccttatt aaacttcggc agcatagaga
    21181 taaattcgtt aaacgatgcc gccagtacgc cgaccagcgc agttagtgcg atcagcatca
    21241 ccaccaccac aatcgtaatg gcaatggggc gttgtactcc ccgacgaata aaccaggtga
    21301 cgagcgggtt gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg
    21361 ccgctgcgtg aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca
    21421 tgcccgtttt atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa
    21481 gtgtagcggg agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac
    21541 gtggtaaaag tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc
    21601 gaacccgcgc taaacggatt gcgcctgaat ttgcgcattg tctctatagt catgtttaac
    21661 ttcgccagct acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat
    21721 gtgatgggct ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc
    21781 ttgctgagcc gtcctcatgc cggacgttac gccgatttgc tgggccccaa aaagattgtc
    21841 gtcttcggtt tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc
    21901 gccagtctgc ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt
    21961 gggcaaagtt ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg
    22021 catatcgggc gggtgatttc gtggaacggc attgtcactt acggggcgat ggcgatgggt
    22081 gcgccgttag gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt
    22141 atgggcgtgg cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt
    22201 aaaggcaaac cgctgccgtt tcgcgcggtg cttgggagcg tctggctgta cggtatggcg
    22261 ctggcactgg cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac
    22321 gctaaaggtt gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt
    22381 acgcgtttgt tattccctaa cggcattaac cgtatcggtg gcttaaacgt agcgatgatt
    22441 tgctttagcg ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg
    22501 gcgaaaatcg gcgtcttact ggcgggggcc gggttttcgc tggtgttccc ggcattgggt
    22561 gtagtggcgg taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc
    22621 gtatttatgg atttatcgct tggcgtgact ggaccactgg ctgggctggt gatgagctgg
    22681 gcgggcgtac cggtgattta tctggcggcg gcgggactgg tcgcaatcgc gttattactg
    22741 acgtggcgat taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa
    22801 aacttactga ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg
    22861 atcgtcagcg ggcagcgtaa tttgcatggt cagcagttga ttacccacgt tacccagaat
    22921 aacggaagag tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt
    22981 gtgacctttc agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg
    23041 ttgctgatct tccagacgct tcgccagcac cgccagatct tctttcggat catcgcccat
    23101 gatgacgatg actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg
    23161 cgttcccagc ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc
    23221 atcaagcagg ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt
    23281 agcgttagtg tctttatcat cacaggccgc aagccccata accagcaggc caattccgac
    23341 atatttaacc agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt
    23401 catccatctt atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc
    23461 ggatttatct gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa
    23521 gaaaacgata ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg
    23581 ttcagccata aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc
    23641 actgacgtta ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactttgacg
    23701 caggcggtta aaaacgtgca cgtcgaggat ttgccccagc gcgtaggcca tgaaactggc
    23761 ggtggcgata cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccatga
    23821 acccatatag aacagcgacg agatgacata ggagattaat aacgcaggga tcattaccgc
    23881 gaagataatg cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtcgcgag
    23941 aaagataaac ggaaaactaa acgcgcccca ggtggtatga aaaccaaaaa tggagaccgg
    24001 aagctggacc agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa
    24061 caacgcctta tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc
    24121 attggggtga gggaacccaa tacgtacgac acgtctgtta tcttaacata ataacgtgct
    24181 tacccttttt tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt
    24241 tcacgcaatc gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt
    24301 tttaagtgat gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc
    24361 gcttggcctg cgctgcccgg aaccggtgat gatggtgcgc aaaaccgtgc gcaatatgca
    24421 gcctggcgaa actttactga ttatcgccga cgatccggcg acgacccgcg atattcctgg
    24481 gttttgtacc tttatggaac acgaactggt tgcgaaagag acggatggac tgccttatcg
    24541 ttatttgatt cgtaaaggcg gttgatgggg aaagattggc ttcgatgccg ccttttcccc
    24601 tcaccctaac cctctcccca gaggagcgag gggaccgatc gcgctcaatg ttgcgatcgg
    24661 tttgccttat ctcctgcgca acaatcttaa cgcattcgct gttaccagca cggtcgcacc
    24721 ggtatctgcc agcaccgcca gccatagtcc ggtcatccct agcagcgtgg tgacgaggaa
    24781 gatccctttc agccccagcg caatagtgat gttctggcgg atattggcgt gagtggcgcg
    24841 tgccagttca atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcgtcggc
    24901 ggtttccagc gccacgtctg tgccactgcc cattgcaatc ccaatggcgg cagctttcat
    24961 cgctggcgcg tcgttaatac catcaccgac catcgccagg ggcgcatgtt gattcagctt
    25021 ggtcaccgct ttgactttat cttccggcaa taggcccgct ttaaactcca gccccagctc
    25081 cccggcaatt gccgccgctg cgcgtggatt atcgccggtg aggatcactc ctttaacccc
    25141 cagcgcgttc agttcactga tggcagttgc tgcgtcggcg cgcagggtat cctgcaatgc
    25201 aatgataccc agcacgtcat cattacgtac taccagcaca accgtttgcc cggcgctttc
    25261 cagttcatta atcaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa
    25321 tacgcgctca ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgattc
    25381 ggcggtggga atggcgagtt cagcaacctg tgcttcgcgt acgatggcct gcgccagtgg
    25441 atgcgtcgcg ccttgctcga ccgccgccgc cagtgtcagc agttcagatt cactaatacc
    25501 cgttgccgga tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc
    25561 aaacgccacc tgagtaacac gacccagctg ttccagcgcc gctccgcctt taatcaacgc
    25621 cccacgacgc gctgccgccg ccagcccgga ggtaatcgcc gcaggcgtgg agataactaa
    25681 cgcacacggg cagccaatca gcagcagcgt cagcccttta taaatccact cctgccagct
    25741 ggcggcaaac agcagcggtg gcaccagcgt caccagcagg gcgacggcca taatcgctgg
    25801 cgtataaata cggctgaaac ggtcgataaa ccgctcaatg ggggcgcggc gctcttcggc
    25861 ttcttcgatc agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc
    25921 cagcgttacc agacggtcta cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg
    25981 ctccaccgga atggattctc cggtcagggc gctttcatca aaactggcaa acggtgagag
    26041 cagtttaccg tcggcaggca aacgcccacc tgcggcgact tcaatcacat cgccaggccg
    26101 caggctgtta atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg
    26161 tttcagcgcc attaacgcgc taacgccctg acgcgcgcgg ctggcggccc agccttccag
    26221 tcgttcacca atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa
    26281 cagtgcacca atagcggcta cgctcattaa ggtttcaatg gcgaagtagc tgccggattt
    26341 gatcagccgt aatgcctgac gagcaatcgg gtacagccca accagcgtgg tcgcgataaa
    26401 cgccagttgc ccaaacggat gattaaattg ctccagaccc cagctgattg ccatcatcac
    26461 gattagcgta atcagcggca gattctcttt caggcgcgat gcttgtggct cgtcggcggc
    26521 ctgctcatcg cgcagggaat agcccgcttt ttgcaccgca gattcaacct gggcacggat
    26581 gtcattgtcg gcatcgacca ccagtttttc ggtggcgaac agtacctgca cctgattcac
    26641 acctgcaagc tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc
    26701 gctgactttc cagctatagc gggtgccgga gacgttttca gagagcgttg gcgagctgga
    26761 acatgcgccg tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc
    26821 agcaaattga ggggctttct tgccgtgatt gtcaggagtc gacatggcat cctccggtta
    26881 agttttttct cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc
    26941 agagatacag cgagcggacg atgaggaaat gaccggcaaa gtagcaggcc gcagcaatcg
    27001 cgttatctgc ccggaagcgg cggcgatagt ggctacccag ccagacaaag ttactgataa
    27061 acagcaatga agcgccaaca aacgcagaga gtgccggagc ggtcggacgg aagaaccaca
    27121 gttcacctgc cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac
    27181 gatactcttc cagtcgcgtc cagataatcg ccagtaacag cgcgcccaga accagcaaca
    27241 ccagtggcag aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca
    27301 gcaggtgcga gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg
    27361 ttagcgcatc gcccagcagt gaggcgcaca gccctgccag caccagatag ctaatggcgt
    27421 cgaacatcgg cgcttgccag gccagtaaca gcaggagaag aagggttaac ggtttaaaca
    27481 cccagcgttg ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac
    27541 agacagcgat aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg
    27601 gaatgcctac ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa
    27661 ctggcagggt atgcttattt ccgcattttc cgatgaggga taaagatgag caaaccacct
    27721 cttttcttta ttgttatcat tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa
    27781 cagcgacggg aaaaagctga taatgatatg gctccgctcc agcaaaagct ggtggtggtg
    27841 agcaacaagc gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg
    27901 gcaggcacca gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag
    27961 acgtttcgcc tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg
    28021 agctataaag gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact
    28081 ttaaatttct tctgccagac cagcagttca aacacgccga aaaggaaaat ccgcaccttt
    28141 tcggcggtgg tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagttgc
    28201 ataccatgca tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcgggcgc
    28261 gggaagggat gcaccagatt gaggattaaa aatccccaaa cgcagagcat taacaaacga
    28321 ccaatattaa tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac
    28381 ctgacccgcc actttttccc gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt
    28441 ttcgacttcg ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag
    28501 taaatttatc gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt
    28561 atgcggtgtg cctttttgcg ccaggaatga cattgcgttg ctgttcacca cgcgtgcatt
    28621 gcctgctttt agtgtcgcca gattcttaat taactgctga gaaactgcgc gatccatctc
    28681 aatcaacgtg gcccccgcag cgtagcgcga taacgcttcc agccccagcg cgccgctccc
    28741 ggcgaagcaa tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt
    28801 ttcgcgtacg cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg
    28861 gcctcgccac tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt
    28921 catgataaat tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag
    28981 ttaagtgata gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg
    29041 ccatgaagca acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt
    29101 tttcctggct gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga
    29161 atgaacaacc ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttctgaac
    29221 aagccgtcga agagcagccg caggcgcata ctgaagccga ggcggaaact tttgctgccg
    29281 acgttgtgga agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag
    29341 tcgttgcaca gccggaaccg gtcgtagaag aaacgccgga gccagtggct atcgagcgtg
    29401 aagagctgcc gttgccggaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg
    29461 ctgaagcgga aaccgtagag attgtcgaag cggcggaaga agaagcggct aaagaagaaa
    29521 tcaccgacga agagctggaa gcacaggcgc tggctgccga agcggcagaa gaggcggtga
    29581 tggtggttcc tccggtagaa gagcagccgg tggaagaaat cgctcaggag caggaaaaac
    29641 cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc
    29701 tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgagg
    29761 agctggaaga acagctgttg atcgccgatg tgggcgtgga aaccacacgt aaaattatca
    29821 ccaatctgac ggaaggcgca tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc
    29881 tgctgaaaga agagatgggc gagattctgg cgaaagtcga tgagccgctg aatgttgaag
    29941 gtaaaacgcc gttcgtgatc ctgatggtgg gcgtcaacgg tgtgggtaaa accacgacga
    30001 ttggtaagct ggcgcgtcag tttgagcagc agggtaaatc ggtgatgctg gcggcgggcg
    30061 atactttccg tgcagcagcg gttgaacagc ttcaggtctg gggtcagcgc aacaatattc
    30121 cggtgattgc ccagcatact ggtgcggatt ccgcctctgt tatcttcgac gccattcagg
    30181 cagctaaagc gcgtaatatc gacgtcctga ttgccgatac cgccggacgt ctgcagaaca
    30241 aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg
    30301 aagcgccgca tgaagttatg ctgactatcg atgccagcac cgggcagaac gcggtaagcc
    30361 aggccaaact gttccatgaa gctgttggct taaccggcat cacgctaacg aaactggacg
    30421 gcacggcgaa aggcggggta attttctcgg tggctgacca gtttggtatc cctatccgct
    30481 acattggtgt cggcgaacgt attgaggatt tgcgtccgtt taaggcggac gactttatag
    30541 aggcactttt tgcccgagag gattaacaat gattcgcttt gaacatgtca gcaaggctta
    30601 tctcggtggg agacaggcgc tgcagggcgt tacgttccat atgcagccgg gtgagatggc
    30661 gtttctgacc ggtcattccg gcgcagggaa aagtaccctc ctgaagctga tctgtgggat
    30721 tgagcggccc agcgccggga aaatctggtt tagcggccat gacatcacgc gtctgaaaaa
    30781 ccgtgaagtt ccgtttctgc gccgccagat tggcatgatt ttccaggatc accatctact
    30841 gatggaccgt actgtctacg ataacgtggc aatcccgctg attatcgccg gtgccagcgg
    30901 tgacgatatt cgtcgccggg tgtcggcggc actggataaa gtcgggctac tggacaaagc
    30961 gaagaacttc cctattcagc tttctggcgg tgaacaacag cgtgttggca ttgcccgcgc
    31021 ggtggtgaac aagcccgcgg tactgctggc ggacgaaccg actggtaacc tggacgacgc
    31081 gttgtcggaa gggattctac gtctgtttga agagtttaac cgcgttgggg taaccgtatt
    31141 gatggcaacg cacgacatca acctgatctc gcggcgttcc tatcgcatgc tcaccctgag
    31201 cgatggtcac ttgcatggag gcgtgggcca tgaataagcg cgatgcaatc aatcatattc
    31261 ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt cggcggctct ggcgacggcg
    31321 gtcgtaacgc gccaaaacgc gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg
    31381 ttttcaacga acaggtgcgc tatgccttcc acggcgcatt gcaggatctg aaaagcaaac
    31441 cgttcgccac gtttttaacg gtgatggtta tcgccatctc cctgacgctg cccagcgtct
    31501 gttatatggt gtacaaaaac gttaaccagg cggcgacgca atattatccg tcaccgcaaa
    31561 tcactgttta cttgcaaaaa acgctggacg atgacgccgc agcaggcgtg gtagcacagc
    31621 tacaggccga gcagggcgtg gagaaagtga actatctttc tcgtgaagac gcactgggcg
    31681 agttccgtaa ctggtctggt tttggtggtg cgctggatat gctggaagaa aacccgcttc
    31741 cggcagtggc ggtggtgatc ccgaaactcg attttcaggg gacggaatca ctgaatacgc
    31801 tgcgtgatcg tatcacccag attaacggca ttgacgaagt gcggatggat gacagctggt
    31861 ttgcccgtct ggcggcgttg accgggctgg tcgggcgcgt ttccgcgatg atcggcgtgt
    31921 tgatggtggc agcagtgttc ctcgtcatcg gcaacagtgt gcgtctgagc atctttgctc
    31981 gccgtgactc cattaacgtg cagaaactga ttggtgcgac ggatggtttc atcctgcgcc
    32041 cgttcctgta cggaggcgca ttgctgggat tttctggcgc attgctgtca ttaattttgt
    32101 cagaaattct ggtactgcgt ttgtcatcgg cggttgcaga agtggcacag gttttcggaa
    32161 cgaagtttga tatcaatggt ttatcgttcg atgaatgcct gctattgctg ctggtatgct
    32221 cgatgattgg ctgggtggca gcgtggcttg ccacggtaca acatttacgc cactttacgc
    32281 ctgaataata aaagcgtgtt atactctttc cctgcaatgg gttccgtagc agggaaagag
    32341 atcccgttgt ctcttcccgg tatttcatct ctatgtcaca ttttgtgcgt aatttattca
    32401 caagcttgca ttgaacttgt ggataaaatc acggtctgat aaaacagtga atgataacct
    32461 cgttgctctt aagctctggc acagttgttg ctaccactga agcgccagaa gatatcgatt
    32521 gagaggattt gaatgactga caaaatgcaa agtttagctt tagccccagt tggcaacctg
    32581 gattcctaca tccgggcagc taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg
    32641 ctggctgaaa agctgcatta ccatggcgat ctggaagcag ctaaaacgct gatcctgtct
    32701 cacctgcggt ttgttgttca tattgctcgt aattatgcgg gctatggcct gccacaggcg
    32761 gatttgattc aggaaggtaa catcggcctg atgaaagcag tgcgccgttt caacccggaa
    32821 gtgggtgtgc gcctggtctc cttcgccgtt cactggatca aagcagagat ccacgaatac
    32881 gttctgcgta actggcgtat cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc
    32941 ttcaacctgc gtaaaactaa gcagcgtctg ggctggttta accaggatga agtcgaaatg
    33001 gtggcccgtg aactgggcgt aaccagcaaa gacgttcgtg agatggaatc acgtatggcg
    33061 gcacaggaca tgacctttga cctgtcttcc gacgacgatt ccgacagcca gccgatggca
    33121 ccggtgctct atctgcagga taaatcatct aactttgccg acggcatcga agatgataac
    33181 tgggaagagc aggcggcaaa ccgtctgacc gacgcgatgc aaggtctgga cgaacgcagc
    33241 caggacatca tccgcgcgcg ctggctggac gaagacaaca agtccacgtt gcaggaactg
    33301 gctgaccgtt acggtgtttc cgctgaacgt gtgcgccaac tggaaaagaa cgcgatgaaa
    33361 aaacttcgcg ccgctattga agcgtaattt ccgctattaa gcagagaacc ctggatgaga
    33421 gtccggggtt tttgtttttt gggcctctac aataatcaat tccccctccg gcaaaacgtc
    33481 aatccccacg cagattgtta ataaactgtc aaaatagcta ttccaatatc ataaaaatgg
    33541 ggtatgtttt agcagagtat gctgctaaag cacgggtagt catgcataaa acgaaataaa
    33601 gtgctgaatc acaacaccac aacacacgta ataaccagaa gaatggggat tctcaggatg
    33661 aacataaagg gtaaagcgtt actggcagga tgtatcgcgc tggcattcag caatatggct
    33721 ctggcagaag atattaaagt cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac
    33781 ggtgaccagg agtttaccgg cgcagagcag gcggttgcgg atatcaacgc taaaggcggc
    33841 attaaaggca acaaactgca aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg
    33901 gttgcggtgg cgaacaaagt cgttaacgac ggcattaaat atgtgattgg tcacctctgt
    33961 tcttcatcaa cgcagcctgc gtctgacatc tacgaagacg aaggcatttt aatgatcacc
    34021 ccagcggcaa ccgcgccgga gctgaccgcc cgtggctatc agctgatcct gcgcaccacc
    34081 ggcctggact ccgaccaggg gccgacggcg gcgaaatata ttcttgagaa agtgaaaccg
    34141 cagcgtattg ctatcgttca cgacaaacag caatacggcg aaggtctggc gcgagcggtg
    34201 caggacggcc tgaagaaagg caatgctaac gtggtgttct ttgatggtat caccgccggg
    34261 gaaaaagatt tctcaacgct ggtggcccgt ctgaaaaaag agaatatcga cttcgtttac
    34321 tacggcggtt atcacccgga aatggggcaa atcctgcgtc aggcacgcgc ggcagggttg
    34381 aaaactcagt ttatggggcc ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc
    34441 gaatcagcgg aagggctgct ggtgaccaag ccgaagaact acgatcaggt tccggcgaac
    34501 aaacccattg ttgacgccat caaagcgaaa aaacaggacc caagcggcgc attcgtttgg
    34561 accacctacg ccgcgctgca atctttgcag gcgggcctga atcagtctga cgatccggct
    34621 gagatcgcca aatacctgaa agcgaactcc gtggataccg tgatgggtcc gctgacgtgg
    34681 gatgagaaag gtgatctgaa aggctttgag ttcggcgtat ttgactggca cgccaacggc
    34741 acggcgaccg atgcgaagta atcattaatc ggcaactttg ggttgccgcc aaattgctaa
    34801 tatcgagtac gttgcttcat gccggatgcg gcgtaaacgc cttatccggc ctacaagatc
    34861 caaagaaatc agtaaattgc aacacacatt gtaggcctga taagcgtagc gcatcaggca
    34921 atttctcgtt ggtcatcact tttgaaatca acttgacgat taacgcttct cccagccgcc
    34981 ctgttgcgcc gtaaacccca gcgcctgcat aaacgccgtc atcacaccgc gatcttccac
    35041 gcctgcatcc gccatccacc agcatgaaac ggcaggattg ttacgcaaaa cctcttccag
    35101 cagatattgc cccacaccgc gacggcgggt gacttcccgc acgcgcaggg aatccagtgc
    35161 tccctcggtg ccgcttaagg ttacccgcac ggccgctagc aggcgctcgt taaaacgcgc
    35221 ggcgtagatg cggtggttat cgtcaacctg taaagaggaa ggggaatact ccggccagat
    35281 cttttgcagg tcaatccggt cctggtcgct aaagttttct aatcgaatga tggtcagctt
    35341 catgggtaac ccgtgtaaat cacaaaagtg gaaccagtgt agcgaaataa tttaatcgga
    35401 ggctttctct tttttatttc ttttggcagg tgattaattt tttaacagca ataattacaa
    35461 agttaaaaca ttatagaatg aaaaataccc agcataatcc cctgaatgat agtgaattat
    35521 tccgcccttt gtgccgttat tttatgctga caaaggcact tttttctgtt tatctatcaa
    35581 taaattcaga atattatctg ttcttaatcg
//
LOCUS       DAZFYC010000038        34978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFYC010000038 DAZFYC010000000
VERSION     DAZFYC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.038"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            189..773
                     /gene="yadN"
                     /locus_tag="ACHKW5_002110"
     CDS             189..773
                     /gene="yadN"
                     /locus_tag="ACHKW5_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7511506.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            878..1618
                     /gene="yadV"
                     /locus_tag="ACHKW5_002111"
     CDS             878..1618
                     /gene="yadV"
                     /locus_tag="ACHKW5_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7511507.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1653..4253
                     /gene="htrE"
                     /locus_tag="ACHKW5_002112"
     CDS             1653..4253
                     /gene="htrE"
                     /locus_tag="ACHKW5_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO7511508.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAEPVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4270..4839
                     /gene="yadM"
                     /locus_tag="ACHKW5_002113"
     CDS             4270..4839
                     /gene="yadM"
                     /locus_tag="ACHKW5_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7511509.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4854..5456
                     /gene="yadL"
                     /locus_tag="ACHKW5_002114"
     CDS             4854..5456
                     /gene="yadL"
                     /locus_tag="ACHKW5_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO7511510.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5483..6079
                     /locus_tag="ACHKW5_002115"
     CDS             5483..6079
                     /locus_tag="ACHKW5_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO7511511.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6063..7406
                     /locus_tag="ACHKW5_002116"
     CDS             6063..7406
                     /locus_tag="ACHKW5_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO7511512.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7519..8313
                     /gene="panB"
                     /locus_tag="ACHKW5_002117"
     CDS             7519..8313
                     /gene="panB"
                     /locus_tag="ACHKW5_002117"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO7511513.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8325..9176
                     /gene="panC"
                     /locus_tag="ACHKW5_002118"
     CDS             8325..9176
                     /gene="panC"
                     /locus_tag="ACHKW5_002118"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO7511514.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9250..10152)
                     /gene="rpnC"
                     /locus_tag="ACHKW5_002119"
     CDS             complement(9250..10152)
                     /gene="rpnC"
                     /locus_tag="ACHKW5_002119"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO7511515.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            10426..10806
                     /gene="panD"
                     /locus_tag="ACHKW5_002120"
     CDS             10426..10806
                     /gene="panD"
                     /locus_tag="ACHKW5_002120"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO7511516.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10810..12039)
                     /gene="yadE"
                     /locus_tag="ACHKW5_002121"
     CDS             complement(10810..12039)
                     /gene="yadE"
                     /locus_tag="ACHKW5_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7511517.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12103..12543)
                     /gene="yadI"
                     /locus_tag="ACHKW5_002122"
     CDS             complement(12103..12543)
                     /gene="yadI"
                     /locus_tag="ACHKW5_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7511518.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12648..13418)
                     /gene="yadH"
                     /locus_tag="ACHKW5_002123"
     CDS             complement(12648..13418)
                     /gene="yadH"
                     /locus_tag="ACHKW5_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7511519.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13415..14341)
                     /gene="yadG"
                     /locus_tag="ACHKW5_002124"
     CDS             complement(13415..14341)
                     /gene="yadG"
                     /locus_tag="ACHKW5_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7511520.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14450..15112
                     /gene="can"
                     /locus_tag="ACHKW5_002125"
     CDS             14450..15112
                     /gene="can"
                     /locus_tag="ACHKW5_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO7511521.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15153..15689)
                     /gene="hpt"
                     /locus_tag="ACHKW5_002126"
     CDS             complement(15153..15689)
                     /gene="hpt"
                     /locus_tag="ACHKW5_002126"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO7511522.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15895..18285
                     /gene="gcd"
                     /locus_tag="ACHKW5_002127"
     CDS             15895..18285
                     /gene="gcd"
                     /locus_tag="ACHKW5_002127"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO7511523.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18332..19882)
                     /gene="cueO"
                     /locus_tag="ACHKW5_002128"
     CDS             complement(18332..19882)
                     /gene="cueO"
                     /locus_tag="ACHKW5_002128"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO7511524.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20048..20395
                     /gene="yacC"
                     /locus_tag="ACHKW5_002129"
     CDS             20048..20395
                     /gene="yacC"
                     /locus_tag="ACHKW5_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO7511525.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20501..21367
                     /gene="speE"
                     /locus_tag="ACHKW5_002130"
     CDS             20501..21367
                     /gene="speE"
                     /locus_tag="ACHKW5_002130"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO7511526.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21383..22177
                     /gene="speD"
                     /locus_tag="ACHKW5_002131"
     CDS             21383..22177
                     /gene="speD"
                     /locus_tag="ACHKW5_002131"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO7511527.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22215..22577)
                     /gene="yacL"
                     /locus_tag="ACHKW5_002132"
     CDS             complement(22215..22577)
                     /gene="yacL"
                     /locus_tag="ACHKW5_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO7511528.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVIVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22753..25350)
                     /gene="acnB"
                     /locus_tag="ACHKW5_002133"
     CDS             complement(22753..25350)
                     /gene="acnB"
                     /locus_tag="ACHKW5_002133"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO7511529.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAVVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25705..27462
                     /gene="yacH"
                     /locus_tag="ACHKW5_002134"
     CDS             25705..27462
                     /gene="yacH"
                     /locus_tag="ACHKW5_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO7511530.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27533..28957)
                     /gene="lpdA"
                     /locus_tag="ACHKW5_002135"
     CDS             complement(27533..28957)
                     /gene="lpdA"
                     /locus_tag="ACHKW5_002135"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO7511531.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29282..31174)
                     /gene="aceF"
                     /locus_tag="ACHKW5_002136"
     CDS             complement(29282..31174)
                     /gene="aceF"
                     /locus_tag="ACHKW5_002136"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO7511532.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKIAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31189..33852)
                     /gene="aceE"
                     /locus_tag="ACHKW5_002137"
     CDS             complement(31189..33852)
                     /gene="aceE"
                     /locus_tag="ACHKW5_002137"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO7511533.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34013..34777)
                     /gene="pdhR"
                     /locus_tag="ACHKW5_002138"
     CDS             complement(34013..34777)
                     /gene="pdhR"
                     /locus_tag="ACHKW5_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO7511534.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8797 a         9041 c         8411 g         8729 t
ORIGIN      
        1 taagaaaatt taatttgttc aaaagccctt aaagtgcaca ataggcgtcc taatgaacaa
       61 tggttgtaaa ttttggtaac aaatatcatt gctcattaat atctagcatc tgcttataac
      121 atttgtgtca tgaatagtca ttcttcaaac cagtagtctg actaatggaa ttttattcaa
      181 ggatttttat gtctaaaaaa ttaggttttg ccctgtctgg tcttatgctg gcaatggttg
      241 caggtactgc ttctgctgat atggatggtg gtcagttaaa tatcagtggt ctggttgttg
      301 ataacacctg tgaaacccgc gttgacggcg gtaataaaga tggtctgatc ctgctgcaaa
      361 ctgcaactgt tggtgaaatc gatgctggcg tgctgaatga caccgttggc gctaaagcca
      421 aacctttcag catcaccgtt gactgcagca aagcaaatcc gaatccaggt agtacagcta
      481 aaatgacctt cggttctgtt ttctttggta atagcaaagg aacgctgaac aacgacatgt
      541 ctattaacaa cccgtctgac ggcgtgaata tcgctctgca caatattgat ggttccacta
      601 tcaaacaggt tcaaattaac aaccctggcg atgtgtatac taaagccctg gatgcaacga
      661 caaaatctgc tgtttatgat tttaaagcgt cttacgttcg tgccgtcgca gaccaaacag
      721 caactgctgg ttatgtaaaa actaacactg catacaccat tacttatcag taagtattca
      781 ccaattaaat tgattgaatg aatatacagg gaatagtgtt aataatttct atttttatat
      841 tattccctgt tttaattaac tctgtcaggg atggtttatg ttttttaaca ccaaacatac
      901 aacagcttta tgcttcgtaa cctgtatggc ttttagttca tcgtctattg cggacattgt
      961 catttcgggt actcgcgtaa tatataaaag cgatcaaaaa agtgtcaacg tacgtctgga
     1021 aaataaaggg aataacccgt tgcttgtcca gagttggtta gatactggcg atgacaacgc
     1081 tgagcctggc agtattacag tcccttttac tgccacgccg ccagtatcgc gtattgatgc
     1141 caaacgtggg caaacaatca aattaatgta cacagccagc acctcactgc ctaaagacag
     1201 agagagcgtg ttctggttta acgtactgga agttccacca aaaccagatg cagaaaaggt
     1261 cgcgaatcaa agtctgctgc aactggcatt tcgcacacgt ataaaacttt tctatcgccc
     1321 ggatggattg aagggaaatc cctctgaagc cccgttagcc ctgaagtggt tctggtcagg
     1381 ttcagaaggt aaggcgtcat tgcgagtgac caatccaacc ccttactacg tctcttttag
     1441 cagtggtgat ttagaagcta gcggtaaacg ctatccgatt gatgtgaaaa tgattgcacc
     1501 atttagtgat gaggtcatga aagtcactgg ccttaatggc aaagcaagtt ctgcaaaagt
     1561 acatttttac gccattaatg attttggtgg cgccattgaa ggtaatgcca gtctgtaatc
     1621 aggcaaggat ataattccgc aggaagcata acgtgactat aaaaccaact aaaaattatc
     1681 ataatcatct gacccgtatc gctacgtttt gcgcgctgct gtattgcaat agtgctttct
     1741 gtgctgaacc tgttgaatat gaccatacct tcctgatggg gcagaatgcc tcaaatattg
     1801 atctcagccg gtatagtgaa ggtaaccctg ctataccggg tatgtacgac gtcagtgttt
     1861 atgtcaacga tcaacctatt attaaccaaa gcattacatt tattgaaatt gaaggaaaaa
     1921 aaaacgcaca agcctgtatc acacttaaga atttattgca gtttcatata aattctcccg
     1981 atataaataa cgaaaaagcc gttctgcttg ccagggatga aacactcggc aattgcctta
     2041 atttgacgga aattatccct caggcttctg ttcgttatga cgttaacgag caacgtctgg
     2101 atatagacgt tcctcaggca tgggtaatga aaaattacca aaactatgtt gatccatcgt
     2161 tatgggaaaa tggcattaat gcggccatgt tgtcatacaa cctcaacgga tatcatagtg
     2221 aaactcctgg tcgaagaaat gacagcattt atgctgcttt taacggtggg atgaatttag
     2281 gtgcatggcg actgcgtgcc tcaggcaact acaactggat gaccgattct ggcagtaatt
     2341 atgattttaa gaatcggtat atacagcgtg atatcgcctc gctgcgttct caactcattc
     2401 ttggtgagtc ttatacaacg ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt
     2461 tatacagcga cagccgcatg ttgcctccga cattagcgag ttttgcgcct atcattcatg
     2521 gcgttgctaa taccaacgcc aaagtcacta ttacgcaagg tggctataag atttatgaaa
     2581 cgactgtgcc gccaggcgct ttcgtcattg atgatctgag tccgtcaggt tacggcagcg
     2641 atcttattgt tactgtcgaa gaatccgatg gctcaaagcg gacattctcg caaccttttt
     2701 catcggtcgt tcaaatgtta cgccctggcg ttggacgttg ggatattagc ggcggccagg
     2761 tcttaaaaga tgatattcag gatgagccta atttatttca ggccagctac tactacggtc
     2821 tgaataacta tctaacgggt tatactggta ttcagattac cgataataac tataccgctg
     2881 gtttgttagg tcttggtctg aatacttcag tcggtgcatt ttctttcgat gtgactcact
     2941 ccaatgttcg tataccggat gataaaacat atcagggaca aagttatcgt gtttcctgga
     3001 acaagttatt cgaagaaaca agtacttcac tgaatatcgc ggcctatcgc tattcgacac
     3061 agaattacct tggtcttaat gatgcactaa ctctaattga tgaagtgaaa catcccgaac
     3121 aagatcttga accgaaatcc atgcgtaatt actcgcgcat gaaaaatcag gttacggtca
     3181 gtattaacca accgctaaaa tttgagaaaa aagattacgg ttcattttat ctttccggaa
     3241 gttggtccga ttactgggct tccggacaaa atcgtagcaa ttactctatt ggctacagta
     3301 acagtgcatc ctggggcagc tacagtgtca gtgcccagcg ttcatggaat gaagacggcg
     3361 acactgacga tagcgtttat ctcagtttca ccattcctat tgaaaaatta cttggcactg
     3421 aacaacggac ttcaggtttc cagagtattg atactcaaat gagcagtgac tttaagggta
     3481 ataaccaact caacgtcagt agcagtggct atagtgataa cgctcgcgtc agttatagcg
     3541 taaatactgg ctatacgatg aataaagcca gcaaagattt gagttatgtt gggggttatg
     3601 ccagctatga atcaccgtgg ggaagcctgg caggttcagt ttctgcaaat agcgataaca
     3661 gtcgtcaagt gtctctcagc accgacggtg gttttgtatt gcatagcggc ggcctgacct
     3721 tcagtaatga cagttttagc gactccgata cactggcggt agttcaggct ccaggtgctc
     3781 aaggagcgcg aataaattat ggcaacagta ctatcgatcg atggggttat ggtgtcacca
     3841 gcgctctttc tccttatcat gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg
     3901 atgttgaatt aaaaagtacc agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg
     3961 ctgattttga aaccgtgcaa gggcaatcag ccattatgaa catcacacga agtgatggta
     4021 aaaatattcc atttgctgca gatatttatg atgagcaagg caatgtcatt ggtaatgttg
     4081 gacagggtgg acaagcattt gttcgtggta ttgagcagca gggaaatatc agcattaaat
     4141 ggctcgaaga aagtaaaccc gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag
     4201 aaaaaatagc gcaatctatt attctgaatg gaattcggtg tcagattcag taactacaag
     4261 ggacgtcaaa tgataaaaat aacgccacat aaaatagcga tactgatggg aatattatta
     4321 tcaccatcag tatttgcaac ggatattaat gtagagttta cagccactgt caaagcgaca
     4381 acctgtaaca tcacacttac tggtaataac gtcacgaatg atggcaataa taactacaca
     4441 ttgagaatcc ctaagatggg tctggataag atcgcgaata aaacgacaga atctcaggct
     4501 gattttaaac tggttgctag tgggtgcagc agtggcatca gttggattga taccactctg
     4561 accggaaatg catcatcaag ctcacctaag cttattatac cgcagtctgg tgattcatct
     4621 tcgacgacaa gtaatatcgg tatgggtttc aaaaaacgga ctactgatga tgccactttc
     4681 cttaaaccta acagtgcgga aaagatacgc tggagcacag acgagatgca gcccgataag
     4741 ggtcttgaaa tgaccgttgc gctacgtgaa acagatgcag ggcaaggcgt accggggaat
     4801 tttcgtgcac tggcaacgtt taatttcatc tatcaataag aaggataatt aagatgacat
     4861 ttaaaaattt acgttatgga ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg
     4921 taccctctta tgcggcaaca gacagcattg ggctgaccgt gattactacc gtagaaatag
     4981 gtacttgtac tgctacatta gtaaatgact ctgatcagga catttctgtt gttgactttg
     5041 gcgatgtata tatttctgaa atcaatgcca agaccaaagt taaaacattc aaactcaaat
     5101 tcaaagactg tgcgggtatc cccaataaaa aagcacaaat aaaattaacc aagcgagcca
     5161 catgcgaagg tactgctaat gacggtgcgg ggttcgcaaa tggatccaca gccgcagata
     5221 aagcaagtgc cgtcgccgtt gaagtctgga gcaccgaaac tccggcaaca gggagcgcaa
     5281 cacaatttag ttgtgtgaca ccagcatctc aagaggtcac tatctccaat gcagccaacg
     5341 cggttgttta ttatccgatg agtgctcgac tggtcgtcga aaaaaataaa accgtaagca
     5401 atgtcactgc gggtaaattt tctgcacctg ccacatttac agtaacctat aactaacaga
     5461 ctgacagatc caggaactat atatgcatcc cactcaacgt aagctgatga agagaataat
     5521 tctgtttctg tcactacagt ttcccattac ctatccagcc atcgctgggc aggatattga
     5581 ccttgttgcc aatgtaaaaa acagcacttg caaaagcgga atcagtaacc agggtaatat
     5641 tgatcttggc gtcgttgggg tgggatattt ttcagataat gttactcctg aaagctatca
     5701 accaggtgga aaagagttca ctgtcacagt atcagactgt gcattacagg gaactggcga
     5761 tgtgcttaat cagttacata ttgattttag agcccttagc ggtgtcatgg ctgcaggttc
     5821 aaggcaaata tttgccaatg aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt
     5881 ttccattcag gacccgacca atatattcaa tgttattagt tcagcaggaa attctcgttc
     5941 cgtgtatcca gtaatgactt ctgccctgca taattcttca tggaaatttt atgccagaat
     6001 gcaaaaaatc gatcccgcgc tggatgtcat atctggccag gtaatgagtc atatattagt
     6061 tgatgtgtac tacgaataat agatcaaaac aaataactgc gactgttaca gcagtagatg
     6121 gtggagtgat aatgaagaaa ttcttcagac attttctttt tttaattctg tgcttgagtt
     6181 gttataccgc gagtgctggc actgatgata acgtaggata tattgttggg aatagttacg
     6241 gcgttggtcc gtcagatcaa aaatggagag aaacggggcc gaatggcgat gctactgtca
     6301 tattcagata tgcaacaagt acaaataatt tagttttcta caaaccgacg cagcttggac
     6361 ctacaggtgt aaaattacag tggagtcagt tagataccgc ttctggtggt ggttttcttt
     6421 attgcaacag aagtgatagc acaagtggta gcgcaatgcg tattgaaaat gcaatggttg
     6481 actcaggtaa aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt
     6541 acacattatt aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttcac
     6601 ctgggatata cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag
     6661 acgtgttata ttggagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg
     6721 gtatttatca gacccttaca attgaattct atacagatac aaactttgat ccaacagtaa
     6781 ctcaacagat caaattatcc agctcatcaa attatctgta ttcatttaaa gcctatggcg
     6841 caggccaagg tataaatgag catagttatt ttatcaaaat cgatttcgat ctgctcaatg
     6901 tcaagttgac taatcccact tgttttaccg ctatgcttag tggaacttct gtgactggtt
     6961 ctacggtaaa aatgggtgaa tatagtgcag aacaaatcag aaacggtgcc acaccggttc
     7021 cttttgatat ttcacttcaa aactgcgttc gtgtgactaa tattgagaca aaattagttt
     7081 caacaaaggt tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgacg
     7141 cagcaaaagg agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga
     7201 cattgaaacc taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg
     7261 atacaacagg aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc
     7321 aggccacgct acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca
     7381 gtactttcca ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg
     7441 gtaattgata aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat
     7501 catttatcag gacacgttat gaaaccgacc accattgctt cacttcagaa atgtaagcag
     7561 gataaaaagc gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct
     7621 gatgaaggac ttaacgtcat gctggtaggc gattcgctgg gcatgacggt tcagggccac
     7681 gactccaccc ttcccgttac cgtcgaggat atcgcctatc ataccgccgc cgtacgtcgc
     7741 ggcgcgccaa actgcctgct gttggctgac ctgccgttta tggcgtatgc cacgccggaa
     7801 caagcctttg aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag
     7861 ggcggtgagt ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt
     7921 ggtcacttag gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg
     7981 cgcggcgatg aagcgggcga tcaactgctc agcgatgcat tagccttaga agccgctggg
     8041 gcacagctgc tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgaagca
     8101 ctggcgatcc cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg
     8161 atgcacgacg ccttcggcat taccggcggt cacattccta aattcgctaa aaatttcctc
     8221 gccgaaacgg gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc
     8281 gtttatccgg gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa
     8341 ccctgccgct gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc
     8401 tggtgcctac tatgggtaat ctgcacgatg gccatatgaa gctggtcgac gaagccaaag
     8461 cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg
     8521 aagatctggc acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta
     8581 aagtggattt agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc
     8641 atacttacgt tgacgttccc gggctttcga ccatgctgga aggcgcgagc cgtccggggc
     8701 attttcgcgg cgtttcgact atcgtcagca agctgtttaa cctggtccag ccggacatcg
     8761 cctgcttcgg tgaaaaggac tttcagcaac tggcgctgat ccgcaaaatg gttgccgata
     8821 tgggcttcga tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc
     8881 taagttcccg taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca
     8941 aagttttaag ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta
     9001 ttaccattgc ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc
     9061 gcgatgccga cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag
     9121 ccgcctggct tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact
     9181 taactggcgc tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac
     9241 acaggtaact taaataaggt taattacctt atcaatctca gcaagagata aatttgtcat
     9301 ctctgcaacg tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct
     9361 ttcctcctgt cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc
     9421 cagcgtcatc atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc
     9481 cgcttgttca gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc
     9541 gctagtgtac ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg
     9601 ctggcgaata tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc
     9661 gtcatccggt gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc
     9721 cagctccggc gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc
     9781 gccctgataa aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg
     9841 ccggtgcatg gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt
     9901 gctttggtgt tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata
     9961 gagcacgtcc gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg
    10021 aagcgtgttg aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc
    10081 gcgagccgtc tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact
    10141 cggtgcatcc atcttccctg tcttccgtgt tgtcttgcgt gaaggcagta tccagtcaag
    10201 gcgcgccgtc gtcagccgcc tctttagtgt ttaccgagca gcgttcagag aaatttcaat
    10261 tcgttacagg cgatacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc
    10321 atcagatgag tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc
    10381 aggcaccgcc tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg
    10441 ctgcagggca aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct
    10501 tgcgccattg accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat
    10561 atctggaatg tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt
    10621 tcgagaatta tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc
    10681 atcatcgcca gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc
    10741 gcctattttg aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt
    10801 gcttgattgt tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc
    10861 cgttcttaag atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc
    10921 tttcatggtt gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc
    10981 attaaatccg ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc
    11041 gcggcgtgaa cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg
    11101 tcggcgatag ccatccaccc gatgcaaaaa atgggtatgt gactgaaaat caaaaacatc
    11161 gcgaatttcg ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg
    11221 cgggtgacgt ttgatgcgcg aggtaacaac aaacgccgtc gccttcatgc catattgttt
    11281 caatacagga tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac
    11341 cgctcgcgca gggagattga tcttattctt cacgtaacct tccagctgcg ccatgctcag
    11401 tgtcgcgtat cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtaccgaggt
    11461 ggtcgtcgaa gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt
    11521 tagcaccggc aggccattat cgggttgggc atccagcgcg ctgatatagg cgagtcgatc
    11581 gccaatacgg atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg
    11641 gtagcgcaaa ttgtccgcca gtaccccaaa tggcgcactg cccgcactcg gcgcgttata
    11701 gaccggcgta tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc
    11761 caaaccgtct tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa
    11821 ccctttacca aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat
    11881 gatttgcccg gcccgaatat tccccacggt caccatcttg tcgccaattt gcgcccagat
    11941 cgcagcattt tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt
    12001 aaacagcatc agcaggagaa taacagcttg tttgtacatg aaaaaataac cgagggtgaa
    12061 acaacgcttt tattgtagca aaagcgctat aagaatcgtg ggttaatact catacaaatt
    12121 atgtttgagg acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg
    12181 cccacccagt tcgacaatac gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc
    12241 catcatctgt tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt
    12301 gtggcttaat aatgaagcca cgcggtacgg tggcgctccg gctatatcgg ttaagaagat
    12361 gacaccctca ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt
    12421 agagcttaac ccacgccaga aattcacggc ccggcactga agaagcgccc catgtttttt
    12481 ctccagcgca tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa
    12541 catcgccttc ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat
    12601 agccgtcaaa actcctctcc cccaaatcca gaggagagga aatagcctta gctacgcaaa
    12661 ccacgtccac gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc
    12721 agtacgccaa aggtagtgac cagcggaaca tcattgatac cgaggaagcc gtagcggaaa
    12781 ccactgatca tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac
    12841 aaagtcagtg agtaaaagac cccacccaaa tacgtgagtg gcgttaacac aaaggttggc
    12901 accaggctga tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag
    12961 aatagcaccg ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac
    13021 ggcacaaaaa acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg
    13081 ccaccgccga catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct
    13141 tcaatattac gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcattg
    13201 gtgatcaccg acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc
    13261 atatcgccaa tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg
    13321 atgactggcg gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc
    13381 cagatgcttt ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat
    13441 taaccagtga aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct
    13501 gaatgccctg ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgcagcactt
    13561 caacttccag cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg
    13621 gacttttcgg tgcgagatcg agaataaagg tttccgattt cagcttagcc agcagcgcct
    13681 tcatcgaggt attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca
    13741 tttctgcttc ttccaggtag tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat
    13801 ccttcaaaaa gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt
    13861 cgagaatcag tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca
    13921 tcccgccaga taacatacgc gcacgttcgt tgcgttttcc ccatagatcg agttgtttaa
    13981 gatatttttc gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat
    14041 ttaccacaat ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc
    14101 ccaactgacg tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc
    14161 gcccggaggt tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc
    14221 cgttcggccc gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa
    14281 gcgcctgaac gccgcctgga taagtttttt taagctgttg aagttccagt gcaatggtca
    14341 taaattttta cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt
    14401 gccctatatt agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat
    14461 agatacactc atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg
    14521 gttttttgag aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga
    14581 cagtcgcgtt cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg
    14641 taatgttgct aacctggtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc
    14701 agtggatgta ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt
    14761 acaagccgcc gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg
    14821 cgatatctgg ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga
    14881 taccttgtgt gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat
    14941 gcaatcagcg tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca
    15001 cgacggcttg ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg
    15061 ttaccgtcac gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca
    15121 tgccggatgc aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg
    15181 atatacggca gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca
    15241 tccgggatcg agaaaccgat aaattctacc gggacgttca cttcacgacg ggacggttta
    15301 tccagcagcg tacaaatcgc cagcgacttc ggttcgcgca ggcttaagat ctcacgcact
    15361 ttcgacagtg tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg
    15421 atatcttcat ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg
    15481 tagctggagg cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc
    15541 gccataaaca taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct
    15601 ttgtaacgct cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg
    15661 gggatcatta cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca
    15721 cttcgcacgg gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa
    15781 actattcatt cgcggaatct gttaataaag ctcgaattgt gatgacgatc acacatgtta
    15841 aaacctataa ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca
    15901 attaacaata caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt
    15961 tgcgggctgt atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac
    16021 taccctatcg ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt
    16081 gccgcgcttt ggctgtacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa
    16141 gttggtttcg acttctgggc gctgactccg cgcagcgaca ttctggtctt cttcggcatc
    16201 tggctgatcc tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc
    16261 gcactggtgg tggcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat
    16321 ccgcaggaaa tcaacggcac cttaagcgcc aatgccacac ctgcggaagc tatctcaccc
    16381 gttgccgatc aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct
    16441 ctgaaacaaa ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact
    16501 ggcgatgtga agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa
    16561 gtgggcgaca ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc
    16621 agcggcaaag agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac
    16681 gtaacctgcc gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg
    16741 atggcggatt gcccgcgtcg tatcattctt ccggtcaatg atggtcgact gattgcgatt
    16801 aacgctgaaa acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa
    16861 agcaatatgc cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc
    16921 gataaaacca tcgtgatggc cggttcagtt accgataact tctcaacccg cgaaacgtct
    16981 ggcgtgatcc gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc
    17041 gcgaaagatc cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaac
    17101 tcctgggcac cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg
    17161 accacgccgg atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg
    17221 attctggcgc tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac
    17281 gacctgtggg acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt
    17341 cagaaagtgc cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt
    17401 cgcaatggcg aactggtggt tccggccccg gaaaaaccgg tgccgcaagg tgctgcgaaa
    17461 ggcgattacg ttactccgac ccagccgttc tctgaactga gcttccgtcc gaagaaagat
    17521 ttgagcggtg cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg
    17581 ttccaccaga tgcgctatga aggcattttc acgccgccat ctgaacaggg cacgctggtc
    17641 ttcccgggta acctgggaat gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa
    17701 gtggcaattg ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtccg
    17761 ggtaacccga tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag
    17821 ccacagtacg gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc gtttggtctg
    17881 ccgtgtaaac agccagcatg gggttatatc tcggcgctgg atctgaaaac caatgaagtg
    17941 gtgtggaaga aacgtattgg tacgccgcaa gacagtatgc cgttcccgat gcctgtaccg
    18001 gtgccgttca atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg
    18061 ctgtttatcg ctgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa
    18121 aaactgtggc agggccgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg
    18181 aatggcaagc agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg
    18241 ggcgactata ttgtggctta tgcgctgccg gatgatgtga aataaccata atgcccggag
    18301 agatccgggc atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg
    18361 tatcttcatg ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt
    18421 gattaaactt caccagcact tcactgacat tgccttctac cttaacggta tctttccagc
    18481 ccgcgcgatg agccgctggc ggtttgccat tttctgacaa gatacggaac tgggtgccgt
    18541 gaatatggaa cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact
    18601 gccctttcgc cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat
    18661 tggcatggtg gaaatcgaac ttcccgccat ggttcatatg attcatattg ccgtgcccca
    18721 tatggcccat catctggcta tgatccatcc cggccatcgc ctgatcgcca tatttctcca
    18781 tcagcatctg catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta
    18841 ccgtcagccc ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac
    18901 cggaggcact aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg
    18961 caatcgccat ccccatctgg ctgaccggta gcgtcaccag gtcaaagggt ttgttgtcgt
    19021 taacctccac cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca
    19081 ccggttcagg tagcagacca ccgtcgctgg caatcacata caacgggcga ttgtcgctgg
    19141 tggcgaaatt gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac
    19201 gtggggcagc gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc
    19261 ccacggcggc ggtcatcaca tccagttgat aatcaatctg cccatcagcg ttaaattttt
    19321 tatcctgaac aatcaccgga acatcatcga taccccactg ttttggcagc attaatttca
    19381 ggatctcgtc atcttcaatt accaccagcc cagccaaccc catcgccacc tgtcgcccgg
    19441 tcttgccatg ttgatgtgga tggaaccagc aagtagcggc aggttgatca acgttcaacg
    19501 tcaccgagcg cttgccacct ggcggaataa ttccctgcgg gccgccgtcg acttcacccg
    19561 gtacttccag cccgtgccag tgcaacgtcg tctcttccgt cagttggtta tagatatcaa
    19621 ccgttaccgc tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc
    19681 cccaggtagt tgcagttttc tcgccaaagg tagactgacc tgcgccaata gttaactgaa
    19741 tgcgattacg ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg
    19801 caaagactgc gcggctccac agcggcaagg ctgaagccac acccagcgcg acggaatatt
    19861 ttaagaaatc acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca
    19921 aacgttgagc atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga
    19981 caataatggt cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt
    20041 agaagcaatg aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtttgcgc
    20101 aaacagttac gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt
    20161 tgtctttctg aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc
    20221 actggtcttt ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat
    20281 gaaagccctc ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa
    20341 aaagtgcaaa gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct
    20401 gatacctttt tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc
    20461 gccctttttt tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg
    20521 gcatgaaacg ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca
    20581 tgaaaagacc gatcaccaag atctgatcat ttttgagaac gctgcatttg gtcgcgtaat
    20641 ggcgctggat ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat
    20701 gacccatgtt ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg
    20761 cgacggtgcc atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt
    20821 ggaaatcgat gcgggtgtcg tatcgttctg ccgtcagtat ctacccaacc ataacgccgg
    20881 tagctacgac gatccgcgct ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca
    20941 aaccagccag acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga
    21001 aagccttttc acttcggcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat
    21061 cttcgtcgca caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg
    21121 caaactcagc cattacttca gcgacgttgg cttttatcag gcggcgattc cgacctatta
    21181 cggcggtatc atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac
    21241 cgaaattatt caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc
    21301 aatccatacg gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc
    21361 gtcctaagga gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac
    21421 caaaagtctg agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga
    21481 cggttatatt gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc
    21541 agaaacctgt tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc
    21601 acagggtgcc agcgtcacta ttctggtgag tgaagaaccg gtcgacccga aacttatcga
    21661 caaaacagaa caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca
    21721 tatttgcgta catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc
    21781 cgatattgaa gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat
    21841 ccaccagctt gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga
    21901 cattaacggt atgaaacact ttatcgacca tgagattaat tcgattcaga actttatgtc
    21961 tgacgatatg aaggcgctgt atgacatggt ggatgtaaat gtctatcagg aaaatatctt
    22021 ccataccaag atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc
    22081 ggaagattta accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg
    22141 cgagatttat tacgggcgca atatgccagc tgtttaacgt ctcgggcgga gctcccaggc
    22201 tccgccagat ttatttactt ctgctgcacg aaattacggt aagccgccac gacctgcaga
    22261 aaatcctcaa cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc
    22321 atttcatcgc cagcgaattc cagttgattg gcgcgaacaa tcacctcttc accgtccatc
    22381 cacagagtgt actcatgccc tgcccgctgc caggaacgtt cgctaccttt cagtgcgtgc
    22441 gccgcttgtt ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag
    22501 tgcccgacca cttcatgccc catggacata cgcaccttta caactccggt aatatcgcgc
    22561 agaaattcgt aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcattgc
    22621 gccattgatg taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag
    22681 aaataaaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg
    22741 tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg
    22801 agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc
    22861 cacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacaaccgc
    22921 cagttccgca gaagccagga agacattcgc gccagtaccc agacggttcg ggaagttacg
    22981 ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag
    23041 ggaacagcca gggatctcga tacgcgcacc actcttaccg aagacgctgt agtagccttc
    23101 ttcagtcaac tgcgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcag
    23161 ttggcctttg tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttggtcat
    23221 gcaggaaccg ataaatactt cgtcgatctt ctcaccctgt accgcagaca gcggacgcgc
    23281 gtcatccggg tcgttcggtg cacagaggat tggctcttta atatccgcca gatcgatgtc
    23341 gatcactgcc gcgtattcag catctgcatc ggcttccagc agctcaggat tcgccagcca
    23401 tttttccatg ccctgaatac gacgttccag ggtgcgacga tcgccgtaac cttccgcgat
    23461 catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag
    23521 cttgatggta caaccagcgg cagaacgctc ggcggacgca tcggtcagtt caaatgcctg
    23581 ctcaactttc agatccggca gaccttcaat ttccaggatg cggccagaga agatgttttt
    23641 cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg gaatggcatg
    23701 taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga
    23761 ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc
    23821 cgccgggaaa gagataccga tcgggaaacg ggtatgggaa tcaccaccgg taccgacggt
    23881 atccggcagc agcatacggt tcagccagga gtgaatgacg ccgtcacccg gacgcagcga
    23941 cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg
    24001 cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag
    24061 gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct gggaacctac
    24121 agaagtcatt ttcggttcac agtacgcgcc cggacgaatg cctttcacgc cacaggcacg
    24181 gcctaccatt ttttgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg
    24241 acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg
    24301 cccgataatc agcggaatac ggccaccagc acgcacttca tcaatcagca cgtcggtttt
    24361 cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac ctttgtacgg
    24421 gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc
    24481 acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc
    24541 accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt
    24601 agtggcggat ttacgcgaag aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa
    24661 acctttctgt tgcagagctt cgatttgctt gatcggacca acaacaccag gctggtctgg
    24721 ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg
    24781 tgaccacgca tccggtgccg gagaaaggtc atcggtgtta gtttcgccag tgactttgaa
    24841 gacggtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc
    24901 ccaggactgc ataacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc
    24961 atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcaa taggtgccag
    25021 tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc
    25081 cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttcgcgat
    25141 agcagccagg aagcctgctt tgacataggc ggcttcatcg acgcctgggg gaacacggtt
    25201 ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac
    25261 aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc
    25321 agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt
    25381 gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt
    25441 accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac aaaaaagcaa
    25501 cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact
    25561 tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt
    25621 aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt cgccgaccat
    25681 tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg
    25741 gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat aaaaagccgc
    25801 acagtggaag cgcctgtgga accgcaatcg acacaacaga ctgcgcctga catcaccgca
    25861 gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt caaatccgca
    25921 ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc cgattctctg
    25981 ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg
    26041 tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg
    26101 tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa
    26161 aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg
    26221 gactcggtac agcgattgcg gcaactggcg caacaaaccg gttcgctgaa gtcatcaacc
    26281 gaacaaaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggca
    26341 cccgtcatac catccaatac cgttttaact gccagccccg tcattacaga gcctgcaaca
    26401 accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca
    26461 accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca
    26521 gccggagaac cgtttattga cagctttgtg cgcggattcg gctatagcat gggtgttgct
    26581 accacgtacg cactattcag cagcatcgac tgggatgacg acgatcatga ccatcatcat
    26641 catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa
    26701 cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat
    26761 cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat
    26821 catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagcgcc
    26881 acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag
    26941 caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag
    27001 cggtttaatg aagctgaaaa ctatgggagc tatgacgact tccgcgactt cagccgtcgc
    27061 caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc ggcctcgcct
    27121 gagcagcgcc aggcagtccg cgagaaaatg cagactaacc cacagatcca gcagcgaaga
    27181 gacgcagcgc gtgagcgtat tcagtcagcc tcgcctgagc agcgccaggt gtttaaggaa
    27241 aaagtacagc agcgcccact gaaccaacag caacgtgata acgcccgcca gcgtgttcaa
    27301 tcagcatcac ctgaacaacg tcaggttttt cgggaaaaag ttcaggagag ccgcccacaa
    27361 cgtctaaacg acagtaacca tactgtcagg ctgaataacg agcaacggtc agcagtacgc
    27421 gaacgtctct ctgagcgcgg agcaaggcga caggaaaggt aaattgcaga cgtaaaaaaa
    27481 gcggcgtggt tagccgcttt tttaattgcc ggatgttccg gcaaacgaac aattacttct
    27541 tcttcgcttt cgggttcggc aggtcggtaa tgctaccttc gaacacttct gccgccaggc
    27601 ccacagactc gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct tcagcatcac
    27661 aacccatttc gattgccagg ccgatttcac ccagcagctc gccgccgttg gtaccgacaa
    27721 tcgcaccacc gatcacacgg tgagattctt tgtcgaaaat cagcttggtc ataccgtctg
    27781 cgcagtcgga agcgatagca cgaccagaag cagcccacgg gaaggtggcg gtttcatagc
    27841 tgatgccttt ctctttcgct tctttctcag tcagacctac ccatgcaact tctggttcgg
    27901 tataggcgat ggacgggata actttcggat cgaagtagtg tttcttaccg gcgataactt
    27961 cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag catcggctga ccgacgatat
    28021 cgccgatagc aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg cggatgaaac
    28081 cacggtcgtc cacttccacg ccagctttgc ctgcgtcgag gtttttaccg ttcggcacac
    28141 gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc agcgggtgct tttttgcctt
    28201 ccatcgtcac ataaataccg tcttctttcg cttcaacggc ggtaactttg gtttccagca
    28261 tcaggttgaa tttcttgctg atacgcttgg tgaagacttt aacgatgtct ttgtcagctg
    28321 ccgggataac ctggtcgaac atttcaacca cgtcaatctg tgaacccagc gcgtggtata
    28381 cggtgcccat ttccagaccg atgataccgc cacccattac cagcaggcgt tctggtactt
    28441 ctttcagttc cagcgcgtca gtggagtccc agatacgcgg atcttcatgc ggaataaacg
    28501 gcagttggat cgggcgagaa cccgctgcaa tgatcgcatt gtcgaagttg atcacggttt
    28561 tgccgttctc accttcaact tccagggtgt tagccccggt gaatttaccc agaccgttga
    28621 ccactttgac tttgcggcct ttcgccatac cagccagacc accggtcagc tgattgatca
    28681 ctttctcttt ccaggtacga atcttgtcaa tatcggtttt cggttcgccg aagacgatac
    28741 cgtgttcagc cagcgctttg gcttcttcga taacttttgc tacgtgcagc agtgctttag
    28801 aagggataca gccgacgttc aggcaaacac cgccaagggt gttgtaacgt tctacgatta
    28861 cggtttccag acctaaatca gcgcaacgga aggcagcgga gtaacctgcg gggcctgccc
    28921 caagtaccac gacctgagtt ttgatttcag tactcatcat gacctctata tatttatctc
    28981 cggcggtcat acccgtcgtc tttcaggccg cagcggcgtt agcttcacgc cctcacccca
    29041 gtcacttact tatgtaagct cctggggatt ctggcgcttg ctaccttgct gcacccagaa
    29101 atccataggg tatgtgaagt aataccctaa ccaccaccgg gtcgttctat ccgtcggtat
    29161 tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc tattcgctaa
    29221 taacctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc ggctctttta
    29281 cttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta atgaaacggg
    29341 caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc agcatcagac
    29401 gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa acgccgagga
    29461 tagccacttc cggcgcgttc acaatcggcg cgaagtgggt agtacccagg ccgccgatgc
    29521 tggagatggt gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg tcacgcgctt
    29581 tcttagaaat agtcatcagc tcgcgagaca gctcgatgat gcctttcttg ttgacgtctt
    29641 tgaataccgg aacaaccaga ccgttcgggg tatccaccgc cacaccgatg ttgatgtatt
    29701 tcttcagggt cagacgctga ccgtcttccg acaacgaact gttgaagcga ggcatctgct
    29761 caagagctgc agcaacggct ttcatgatga agacaaccgg ggtgatcttc acatccagct
    29821 tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg gtgatatcgg
    29881 ttttgtcgaa gtgagtaaca tgcgggatca tcacccagtt acggctcagg ttcgcaccag
    29941 aaattttctg gatacggccc agttccactt cttcgatttc accaaacttg ctgaagtcca
    30001 ccttcggcca cggcagcatg cctgggatac cgccgccagt cgccgccgga gctgcttctg
    30061 cacgtttgat agcttctttc acgtaagcct gaacgtcttc gcgcaggata cgacctttac
    30121 ggccagtgcc cttcactttc gccaggttaa cgccaaactc gcgtgccaga cggcggatca
    30181 gcggagtcgc gtgaacgtaa gcgtcgtttt ctgcaaattc agatttgcct tccgctttcg
    30241 cagccggtgc tgctgccgga gcttcagctt ttgctgccgg agccggcgct gccgcttcct
    30301 gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc gaagatcata atcagcgagc
    30361 cagttttcac tttatcgcca acgttgactt tcagttcctt cacgacgcct gcaaacggag
    30421 ccgggacttc catagaagct ttatcgcctt ctacggtgat cagtgactgt tcagcggcaa
    30481 ttttgtcgcc cactttcacc atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg
    30541 gaacgttaac ttctttcacg ccagccgctg gtgcaggggc cgctgccgga gccgcttcct
    30601 gtttagccgc cggagctgcc gcgcctgctt cacccgcgac ttcgaagacc ataatcagcg
    30661 agccggtaga cactttgtca cccacgttca ctttgatctc tttcacggtg ccagcaaacg
    30721 gagccggaac ttccatagaa gctttatcgc cttctacggt gatcagcgac tgttcagctt
    30781 caactttatc gccaactttc accaggattt cggtcacttc aacttcgtcg ctgccgatat
    30841 ccggaacgtt aacgtctttt gccgctgcag ccgctggtgc tgctgccgga gctgtttctt
    30901 tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca
    30961 taatcagtgc gccggtctgg gttttatcgc caacagagac tttgatctct ttaacgatac
    31021 ccgcctgcgg agacggaact tccatagagg ctttgtcgcc ttctacggtg atcagcgact
    31081 gttcggcttc aactttgtcg cccactttga ccaggatctc ggtgatttca acttcatcag
    31141 ccccgatgtc cggtactttg atttcgatag ccattattct tttacctctt acgccagacg
    31201 cgggttaact ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt
    31261 atcgatttcg ccacgtttag ccagttcgcc cagcgccgct acaaccacgt aggaagcatc
    31321 aacttcgaag tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc catcagtacc
    31381 cagtacgcgg tagtcgtcag ccggtacgta agtacggacc tgctcagcga acagtttcat
    31441 atagtcggta gatgccactg ccggagcgtc gttcatcacc tgagcgatat acggaacgcg
    31501 cggagtttcc agcgggtgca gcatgttcca gcgttcacaa tcctgaccat ctcgcgccag
    31561 ttcggtgaag gaggtcacgc tataaacgtc agaacctacg ccgtaatctt tcgccaggat
    31621 ctctgccgct tcacggacgt gacgcaggat ggaaccggag cccagcagct gaactttacc
    31681 tttgctacct tcaatggttt cgagtttgta gataccttta cggatacctt cctcagcacc
    31741 ttccggcatt gccggcatgt ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt
    31801 ctcttgtttt tcaccgtaca tacgctccag accgtcatgc ataatgacag caacttcgta
    31861 agcgtaagcc gggtcgtaag agatacagtt cgggatagtc agcgactgaa tgtggctgtg
    31921 accatcttcg tgctgcagac cttcaccgtt cagggtggta cgaccggaag taccgccgat
    31981 caggaagcca cgcgcttgct ggtcgccagc cgcccagcac agatcgccga tacgctggaa
    32041 gccgaacatc gagtaataga tgtagaacgg aatcatcggc agattgttgg tgctgtaaga
    32101 ggtcgccgct gccagccagg aacaacctgc gcccaactcg ttgatccctt cctgcagaat
    32161 ctgacctttc tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg gggtgtactg
    32221 ctgaccgttc gggctgtaaa taccaatctg acggaacaga ccttccatac cgaaagtacg
    32281 cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc gacttgttct tcagcattac
    32341 gttcagggca cgaacgaaag cgatagtggt agagatctct ttgctctgct cttccagcag
    32401 cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg gctgacggct
    32461 tggcagataa ccgtgcagtt tctgacgctg tgcgtgcaga taggtatgct cttcagaacc
    32521 ttccgggaag gtgatgtacg gcagtttttc gatatctgca tcagacaccg gcacattgaa
    32581 acggtcgcgg atgtgacgca cgccgtccat gttcattttc ttaacctggt gcgcgatgtt
    32641 tttaccttca gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa ggattactgt
    32701 cgctttgcct ttggtttcct gcgctttctt gaatgcagcg tagattttct tcggatcgtg
    32761 accaccacgg ttcagtgccc agatctgctc gtcagtccag tctgcaacca gtgctgcggt
    32821 ttcaggatat ttaccgaaga agtgttcacg aacgtacgca ccatctttcg atttgaaggt
    32881 ctggtagtcg ccgtcaacgg tttcgttcat cagctggatc agtttaccgc tggtatcttt
    32941 acgcagcagt tcatcccaac ggctacccca catcactttg atcacgttcc agccagcacc
    33001 ttcgaagatg ccttccagtt cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag
    33061 acgctgcagg ttacagttga taacgaagac caggttatcc agtttttcac gggtagcgat
    33121 ggtgatcgca cctttggatt ccggttcgtc catttcaccg tcgccgagga acgcgtaaac
    33181 ggtctgttta gaggtatctt tcaggccacg gtgttccaga tatttcagga atttagcctg
    33241 gtaaatagca ccaatcggcc ccagacccat agatacggtc gggaactgcc agaattccgg
    33301 catcagtttc gggtgcggat aggaagagag gccattgccg tgaacttcct gacggaagtt
    33361 atccagctgc tcctgagtca gacgaccttc caggaaagca cgagcgtaca cgcccgggga
    33421 gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa
    33481 gtggttaaag cacacatcat aaatggttgc ggaagactgg aaggacgcca tatggccgcc
    33541 cagttcgagg tcttttttcg acgcacgcag caccgtcatg atggcgttcc agcggatagc
    33601 tgaacgaata cggcgttcca gttccagatt acccggatac tccggttgtt cttcaacggg
    33661 gatggtgttg atgtagttgc tgatacctgt gcctgcggct acgtttacac cgcctttgcg
    33721 ggcttcagca agcagttggt cgatcagata ctgagcacgc tcaacacctt cttcacggat
    33781 gaccgattcg atcgcctgga gccagtcgcg agtttcgatc ggatccacgt catttgggaa
    33841 acgttctgac atgggttatt ccttatctat ctaataacgt tgagttttct ggaacctgtc
    33901 ccattgaact ctcgccggaa agctcaataa gacaggttct acgtttagtt gccgcgcttt
    33961 tatatgcgct tgatttacaa catcttctgg ataattttta ccagaaaaat cactaattct
    34021 ttcgttgctc cagacgacgc agagaacgct cacggcggct ctcttcacga cttctgtcga
    34081 gcaaaatttc ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct tcttccggct
    34141 taccggccat aatcgcttca aatatgcggg tgcggtgact actcaccagc ggcagcatct
    34201 cgcgacgcga atagagcaat tcgaagttct ggcggacatt ctgggccaac atcggctcca
    34261 tacaccttag cagatgaagc agaaccacat tgtgggccgc ttcggtgacg gcaatctgat
    34321 actggagtac ggcgtttgat tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg
    34381 cgtggtggag ttcacggatg cgttccttgt cttcatcggt actacgcagc gcggcgtaat
    34441 aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt gactcaggat
    34501 ggtcggagag cagctccacc agcggatcgc tgaagctttg ccataggctg ctctggacaa
    34561 aagtgccgcc accctggcga cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct
    34621 cacgcaagga gggacgggag acgtcaaact gttttgccag ttcgcgttcc ggtgggagtt
    34681 tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc cagttgctgc tcaatcacat
    34741 cggagagttt tggttggcgg attttgctgt aggccatgag ttcctgtctt aagccacttg
    34801 ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc taaagtaaca aagtattcac
    34861 cttatgtcca tacaggtttt gattgaaatc atgaaactgt gcacatttta acaacttgac
    34921 atatataacg tttcaaagtt gtaactatgc acaaatgtag actttacgta ggaaagga
//
LOCUS       DAZFYC010000039        34899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFYC010000039 DAZFYC010000000
VERSION     DAZFYC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.039"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(141..893)
                     /gene="tcyN"
                     /locus_tag="ACHKW5_002139"
     CDS             complement(141..893)
                     /gene="tcyN"
                     /locus_tag="ACHKW5_002139"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO7511535.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(890..1558)
                     /gene="tcyL"
                     /locus_tag="ACHKW5_002140"
     CDS             complement(890..1558)
                     /gene="tcyL"
                     /locus_tag="ACHKW5_002140"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO7511536.1"
                     /translation="MQESIQLVIDSLPFLLKGAEYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1573..2559)
                     /gene="dcyD"
                     /locus_tag="ACHKW5_002141"
     CDS             complement(1573..2559)
                     /gene="dcyD"
                     /locus_tag="ACHKW5_002141"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO7511537.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2664..3464)
                     /gene="tcyJ"
                     /locus_tag="ACHKW5_002142"
     CDS             complement(2664..3464)
                     /gene="tcyJ"
                     /locus_tag="ACHKW5_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7511538.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3552..4103)
                     /gene="fliZ"
                     /locus_tag="ACHKW5_002143"
     CDS             complement(3552..4103)
                     /gene="fliZ"
                     /locus_tag="ACHKW5_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO7511539.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTAREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4149..4868)
                     /gene="fliA"
                     /locus_tag="ACHKW5_002144"
     CDS             complement(4149..4868)
                     /gene="fliA"
                     /locus_tag="ACHKW5_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO7511540.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5033..6526)
                     /locus_tag="ACHKW5_002145"
     CDS             complement(5033..6526)
                     /locus_tag="ACHKW5_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO7511541.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6781..8193
                     /gene="fliD"
                     /locus_tag="ACHKW5_002146"
     CDS             6781..8193
                     /gene="fliD"
                     /locus_tag="ACHKW5_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO7511542.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8208..8618
                     /gene="fliS"
                     /locus_tag="ACHKW5_002147"
     CDS             8208..8618
                     /gene="fliS"
                     /locus_tag="ACHKW5_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO7511543.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8618..8983
                     /gene="fliT"
                     /locus_tag="ACHKW5_002148"
     CDS             8618..8983
                     /gene="fliT"
                     /locus_tag="ACHKW5_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO7511544.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9061..10548
                     /gene="amyA"
                     /locus_tag="ACHKW5_002149"
     CDS             9061..10548
                     /gene="amyA"
                     /locus_tag="ACHKW5_002149"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7511545.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10582..10995)
                     /gene="yedD"
                     /locus_tag="ACHKW5_002150"
     CDS             complement(10582..10995)
                     /gene="yedD"
                     /locus_tag="ACHKW5_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO7511546.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11182..12387
                     /gene="yedE"
                     /locus_tag="ACHKW5_002151"
     CDS             11182..12387
                     /gene="yedE"
                     /locus_tag="ACHKW5_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO7511547.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12384..12617
                     /gene="yedF"
                     /locus_tag="ACHKW5_002152"
     CDS             12384..12617
                     /gene="yedF"
                     /locus_tag="ACHKW5_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO7511548.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12726..13394
                     /gene="yedK"
                     /locus_tag="ACHKW5_002153"
     CDS             12726..13394
                     /gene="yedK"
                     /locus_tag="ACHKW5_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO7511549.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(13787..14101)
                     /gene="fliE"
                     /locus_tag="ACHKW5_002154"
     CDS             complement(13787..14101)
                     /gene="fliE"
                     /locus_tag="ACHKW5_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO7511550.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14316..15974
                     /gene="fliF"
                     /locus_tag="ACHKW5_002155"
     CDS             14316..15974
                     /gene="fliF"
                     /locus_tag="ACHKW5_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO7511551.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMMQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15967..16962
                     /gene="fliG"
                     /locus_tag="ACHKW5_002156"
     CDS             15967..16962
                     /gene="fliG"
                     /locus_tag="ACHKW5_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO7511552.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16955..17641
                     /gene="fliH"
                     /locus_tag="ACHKW5_002157"
     CDS             16955..17641
                     /gene="fliH"
                     /locus_tag="ACHKW5_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO7511553.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17641..19014
                     /gene="fliI"
                     /locus_tag="ACHKW5_002158"
     CDS             17641..19014
                     /gene="fliI"
                     /locus_tag="ACHKW5_002158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO7511554.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFTPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            19033..19476
                     /gene="fliJ"
                     /locus_tag="ACHKW5_002159"
     CDS             19033..19476
                     /gene="fliJ"
                     /locus_tag="ACHKW5_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO7511555.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19473..20600
                     /gene="fliK"
                     /locus_tag="ACHKW5_002160"
     CDS             19473..20600
                     /gene="fliK"
                     /locus_tag="ACHKW5_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO7511556.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            20705..21169
                     /gene="fliL"
                     /locus_tag="ACHKW5_002161"
     CDS             20705..21169
                     /gene="fliL"
                     /locus_tag="ACHKW5_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO7511557.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21174..22178
                     /gene="fliM"
                     /locus_tag="ACHKW5_002162"
     CDS             21174..22178
                     /gene="fliM"
                     /locus_tag="ACHKW5_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO7511558.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22175..22588
                     /gene="fliN"
                     /locus_tag="ACHKW5_002163"
     CDS             22175..22588
                     /gene="fliN"
                     /locus_tag="ACHKW5_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO7511559.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22591..22956
                     /gene="fliO"
                     /locus_tag="ACHKW5_002164"
     CDS             22591..22956
                     /gene="fliO"
                     /locus_tag="ACHKW5_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO7511560.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22956..23693
                     /gene="fliP"
                     /locus_tag="ACHKW5_002165"
     CDS             22956..23693
                     /gene="fliP"
                     /locus_tag="ACHKW5_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO7511561.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23703..23972
                     /gene="fliQ"
                     /locus_tag="ACHKW5_002166"
     CDS             23703..23972
                     /gene="fliQ"
                     /locus_tag="ACHKW5_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO7511562.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23981..24766
                     /gene="fliR"
                     /locus_tag="ACHKW5_002167"
     CDS             23981..24766
                     /gene="fliR"
                     /locus_tag="ACHKW5_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO7511563.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25051..25674
                     /gene="rcsA"
                     /locus_tag="ACHKW5_002168"
     CDS             25051..25674
                     /gene="rcsA"
                     /locus_tag="ACHKW5_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO7511564.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(25718..25906)
                     /gene="dsrB"
                     /locus_tag="ACHKW5_002169"
     CDS             complement(25718..25906)
                     /gene="dsrB"
                     /locus_tag="ACHKW5_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO7511565.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26069..26296
                     /gene="yodD"
                     /locus_tag="ACHKW5_002170"
     CDS             26069..26296
                     /gene="yodD"
                     /locus_tag="ACHKW5_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO7511566.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26594..27409
                     /gene="yedP"
                     /locus_tag="ACHKW5_002171"
     CDS             26594..27409
                     /gene="yedP"
                     /locus_tag="ACHKW5_002171"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO7511567.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDCHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(27406..29100)
                     /gene="dgcQ"
                     /locus_tag="ACHKW5_002172"
     CDS             complement(27406..29100)
                     /gene="dgcQ"
                     /locus_tag="ACHKW5_002172"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO7511568.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPFNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(29271..29453)
                     /gene="yodC"
                     /locus_tag="ACHKW5_002173"
     CDS             complement(29271..29453)
                     /gene="yodC"
                     /locus_tag="ACHKW5_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO7511569.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(29532..30449)
                     /gene="yedI"
                     /locus_tag="ACHKW5_002174"
     CDS             complement(29532..30449)
                     /gene="yedI"
                     /locus_tag="ACHKW5_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO7511570.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            30622..31542
                     /gene="yedA"
                     /locus_tag="ACHKW5_002175"
     CDS             30622..31542
                     /gene="yedA"
                     /locus_tag="ACHKW5_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO7511571.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(31531..32001)
                     /gene="vsr"
                     /locus_tag="ACHKW5_002176"
     CDS             complement(31531..32001)
                     /gene="vsr"
                     /locus_tag="ACHKW5_002176"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO7511572.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(31982..33400)
                     /gene="dcm"
                     /locus_tag="ACHKW5_002177"
     CDS             complement(31982..33400)
                     /gene="dcm"
                     /locus_tag="ACHKW5_002177"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO7511573.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDSRRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33467..34162)
                     /gene="yedJ"
                     /locus_tag="ACHKW5_002178"
     CDS             complement(33467..34162)
                     /gene="yedJ"
                     /locus_tag="ACHKW5_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO7511574.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNYPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(34202..34483)
                     /gene="drpB"
                     /locus_tag="ACHKW5_002179"
     CDS             complement(34202..34483)
                     /gene="drpB"
                     /locus_tag="ACHKW5_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO7511575.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         8533 a         9073 c         8818 g         8475 t
ORIGIN      
        1 ttcaggcaat gaattacttt tgcaagccat cgcattctct tatgttatta atgagttatg
       61 ctgatttgtt aagcagtttt atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg
      121 tcggggctga ttttttctta ttattgcagc agaaacttct cgaggaactg gcgggtgcga
      181 ggctgctggg gatcggcaaa taacgctttt gctgccccct gctcgactat ccgcccctgg
      241 tccataaaga tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg tgtcacaatc
      301 accatcgtgc gcttttcctg cgccagctgg cggatggtgt tcaggacttc gcccaccagc
      361 tctggatcca gcgccgacgt tggctcgtca aacaaaatca cctcaggacg cattgccagt
      421 gcacgcgcaa tcgcaacacg ctgctgttga ccgccagaca aacgacgtgg atagctggtt
      481 tctttaccgg ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct
      541 ttcggttcgc ctttgacgat caccggccct tcaataatgt tttccagcac cgtacgatgc
      601 ggaaacaaat taaagttctg gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc
      661 gatttttgct gacttaatga acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt
      721 cccgcttccg gttgttccag cagatttatg ctgcgtagca acgtggtttt gccggaacca
      781 ctcggaccga taattgccac cacttcgcca ggctttacct cgaggtcgat accgtgcagc
      841 accgtctgac cgtgaaattt tttcaccagg ttcttaactt caatggcact cattttggtt
      901 ctctctcctg gcgattaagt tgattctcaa aatggttctg caacgtcgat aatactgtcg
      961 ccatgatcca gtagatgagc gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg
     1021 aggtaatcaa ctgcgcctga cggaacagct ccggcacctg gattgtcgcg gccagcgacg
     1081 tatcttttac cagactgata aagctgttcg acagcggcgg caacgccacc cgcgccgcct
     1141 gtggcaaaat cgcgcgacgc atggtctgcc acggcgtcat accaatactg gcagcggctt
     1201 cccactgacc tttatcaata gaagaaatgg cggcgcgcag cgtttcggcg gcataggcgg
     1261 cagtgttcag cgacagacca atcattgctg acggaatggg atctaattcg ataccaaact
     1321 gcggcaggcc gtaatagatc ataaacagtt gtgcgatgag cggcgtacca cgaaaaatag
     1381 agatataaaa acgcgccagc cagcgcacgg gccagattgg cgacaggcgc atcagcgcga
     1441 gaataaaccc gagtagtaag ccaaaaaaca tgccgccgat actgagttgc agggtatact
     1501 cagccccttt caacaggaac ggcaaggaat caataaccag ttgtatactt tcttgcatga
     1561 gcgttcccgg agctaaacgt ggggatgata ggcgaacagc gcaggcgcgc cgccggtatg
     1621 aataaacaga atcggccctt catctttgaa gcgtttctga ctgataccgt caatcagccc
     1681 cgccatcgct tttccggtat acacaggatc aagcagaatg ccttccagac gcgccagcaa
     1741 tttcactgct tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc
     1801 ccagagtaaa atttccgctg atgcggtcag ctccagttct ttcgcaatcg cctgttgtag
     1861 gttaaccact ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc caatcagttc
     1921 gctttcaggc atcaggtgtt ccagcccaac agccaatccg gcgtgagttc cggcactgcc
     1981 cgatgcgacc accaccgacg aaatattaac cgccccttca cactgttgcg cgatttccag
     2041 cgcactctcc acataaccca gcgcgcccag agcattagaa ccgccaaccg gaatgacata
     2101 cgggcgaaag ccttgtgctt cgactcgcgt cgccagctct tccagttggg cattgggatc
     2161 ggtcagtgcg tcgcacattt caatctgggt attgaacaga tccagcaaca aacgattgcc
     2221 gttggttaaa tagttttctg cggttgtgcc aataggattt tccagcagcg ccacgcagtg
     2281 cagaccgagt ttcgccgcga ctgcggcagt ctggcgcaca tggttagact ggatcgcgcc
     2341 ggcagtaatc agcgtatcgg caccttcgcg cagggcatct gccgcgagaa attccagctt
     2401 acgtaattta ttgccgccca ttgccatggg tgtgacgtca tcccgtttga tgaaaatttc
     2461 ccgtccaaga taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa
     2521 ctccaaccgt ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtgtgttg
     2581 ttgtgatttt cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc
     2641 gccctttttt gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa
     2701 agagcttgca gagtgccgtc tttttgcatt tccgcaattg catcattcac cgctttcagc
     2761 aggtcttcat ttcctttacg cagcgccacg ccagactcct gacgggagaa tgcttcaccg
     2821 gttactgcca gcgtatcgtt ggttttcttc accagatcca gcgccgccag acgatcaacg
     2881 aggatcgcat cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag
     2941 gtacgcacat cgacgccctg aacattctgc cgcagccact cttcatagtt ggtgcccaga
     3001 ccgacaccca cttttttgcc tttcagatca gcagccgttt taatggtgcc ttcgttacct
     3061 tttttcacca gcgcctgaat accggaaatg gtgtaagggg tagagaaatc gtattttttc
     3121 ttgcgctcgt cagaaatggt gacctgatta atcaccacat caatgcgttt agagtccagc
     3181 gacgccagca taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt
     3241 gccagctgtt gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc tccctgaaaa
     3301 ctgaacggcg gataagtgcc ttccagccct accagcagcg tgccgcgctc tttaacttta
     3361 ttaagcagac cttcatctgc aaaactttta acgctcatgc ccgcaaccag cgctacggcc
     3421 atcacaccca tcaatgcctg acgtcccaga tgtgctaatt tcatatttac cccgaatgtt
     3481 gttatgtctg tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat
     3541 cttattctta tttaatatat atcagaagca ggcaggctgg aggatttctg cacaagtccg
     3601 gtacacgttt gacgctgata gtgttgatac ttccgtaatg cgatgcggta attgttggtg
     3661 ctggtggtgg gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg cagcggatcg
     3721 agggaaatat tttgctcgtg tagatgattc cccaaacggc gcagacgaac gacatattca
     3781 cgcgccgtac cagggctcat ttcagtttgc tcaaaaagaa attgcttaaa gccgataata
     3841 tcgaaaaaat cactctgcgt tttgcaatgt aaatcaccgc agaatcggca caatgccgcc
     3901 catgattttt gttccacttg ccagccattt tcatcaagca gcatgtccag gcgggaaatc
     3961 tcgattttat tcactatttt gccgtcgcga acgagggtaa tgcggtcgag taatttacgg
     4021 caatgcgcgc aatgggtctg gctgtgttta aagtctttaa gatagcggct taatggccgt
     4081 cttttcaggt gctgcaccat cattaagaac tcctggtagt caaaattaaa gtgcggcatt
     4141 tactgacgtt ataacttacc cagtttagtg cgtaaccgtt taatggcctg gctgtgtaac
     4201 tgactgaccc gcgattcccc gacctccagc accgcgccaa tctctttgag attcagctct
     4261 tcctggtaat agagggttaa taccagtttt tcgcgctccg gcaacgtttc gatggcttcc
     4321 atcacccgct ggcgcagatt actgtccagc agttgttgta gcgggttttc tcgctgatga
     4381 tcatcagtaa ccagttcgat gctatcgccg tgctcttcgc gccactcatc gtaggagaag
     4441 agctggctgt tattggtgtc gagcaacatt tggcgataat cggcaatatc gatccctaaa
     4501 cgttccgcta cctcagtttc cgtggcgttg cggccaagtt cctgctccag ttgccctatt
     4561 gcctgtgcca cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga
     4621 agttcatcca gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa tgccgttcct
     4681 tgtagggcgt catagcgttc gacggcatta agtaacccaa tgccgcccgc ctgtagcaga
     4741 tcgtcaagtt ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc
     4801 agcgggacat aacgctgcca cagcgagtgt ttatccatta caccttcagc ggtatagagt
     4861 gaattcacga taaacagccc tgcgttatat gagttatcgg catgattatc cgtttctgca
     4921 gggtttttaa tcggacgatt agtgggtgaa atgaggggtt atttgggggt taccggtaaa
     4981 ttccatgcag aaaaaacccc gccggtggcg gggtttgagc gataagtgta aattaacgca
     5041 gcagagacag tacgttctgc gtggtctggt tagcctgtgc cagaacagaa gtacctgctt
     5101 gttgcaggat ctgcgcacga gacatgttag acacttcggt cgcgtagtca gcatcttcga
     5161 tacggctacg ggcagaagac aggttgttta cggtgttgcc aaggttggtg atggcagagt
     5221 cgaaacgatt ttgaattgca cctaggttag aacgcaacga gtcaacctga gacagtgctt
     5281 tatcgatagc agccaaagga tcttctgttt tctgcgtaga aattgctgca atagaatcag
     5341 ttttcaggct cttaccttgt aaatcactaa cactgtaagt tttgccatcg gttgcagtta
     5401 caacttcggt tttgccatcg cttccaccca gttcaacttt aacattgtcg tgtttgacac
     5461 cgtcagcatc agtgtaagac attgttttaa cggaaactgc tcctgttttc tgatcaacat
     5521 tcgctgcata ctgtttaccg tcatcaccaa tcaacgcaaa tgaaccagta tctttgccat
     5581 tggcgtcttc taacttaaag agttttgcac ctgtgataga agcggttgcg gtatcagtac
     5641 ccaactgaac atcaatagcc cccttccctt tcaccgttgt gacttccttt aaattaccag
     5701 cttgaggctg ggtacccgct agagccccca gcttcagctt acctgcatca ggagatgtcg
     5761 gatcatcctc cacagtaact tcataataac cattttcagc gggtttatct gaaccggcaa
     5821 ctttaacgta caacttaccg ttagcatcag acataatatt accgtcacgt tggcccggag
     5881 tcgtagtgcc tttaactgta gctgtaatat ctgcatcaag tgtattagcg tcagcaagct
     5941 taactgtcga tccaaaatag gttgtttcag ccgttgcggc ttttaattta gtaggatctt
     6001 taataccaaa accagataat cccagtgtgt cggaattaat ttcctgcagt ttaatagtaa
     6061 ttgtttcgcc gtcgtttgca ccaacctgaa tagtcatatc ctgatcagaa gccagcactt
     6121 taacgccgtt aaactgagtc tgaccagaaa cacggtcaat ttcactcaga cgctgctgga
     6181 tttcggactg gatggaggtc aggtcactgt cagagttagt accgttagtt gcctgaacag
     6241 aaagttcacg aatacgctgc aggttgttgt taatttcatt cagcgcacct tcagtggtct
     6301 gcgcaacaga aataccatca ttcgcgttac gggaagcctg agtcagacct ttaatatttg
     6361 ctgtaaaacg gttagcaatc gcctgacctg ctgcgtcatc tttagcactg ttaatacgca
     6421 ggccagaaga gagacgttca atggcggagc tcagagaaga ctgagatttg ttcaggttat
     6481 tctgggtcaa cagcgacagg ctgtttgtat taatgacttg tgccatgatc ttttccttat
     6541 caattacaac ttgatgttat tgggctgttg cccacggttt ctcaccgtac cccttgtatc
     6601 ggcacctgaa tttcgaactt tagaaaattt tagtttctct cctgtttttt cttaacggct
     6661 gaaatatccg taatatacaa ctctactctg cgcatcatct tcgtgaatca actattaaac
     6721 tttgcgcaca aattgccgat aacccgctta tctactgttt gcaatcaaaa ggaattaggt
     6781 atggcttcat ttacatctct tggcgttggt tcgaatctgc cgctggatac attgctaaac
     6841 aacctgacca tcgctgaaaa aaaacgctta aacccaatta ctcagcaaca gagtgacaat
     6901 accgcacgtc tgacagccta tggcacatta aaaagtgcat tagaaaaatt ccagactgca
     6961 aatactgcgt taaataaagc cgatctgttc aaaagcacta ccgttaccag tagcaatgaa
     7021 gaccttaaag tttccaccga agcaggtgct gctcccggta tctataccat cagcgtaacc
     7081 caactggcgc aggcgcaatc tttacgaact gattccccga caatcattgc atcaacaaaa
     7141 gatgcactgg gtgatgagtc atcagataca cgcacaataa aaattaccca agacggtcgt
     7201 aaagagccgc tggaaatcaa gctcaataaa gatcagacgt cattggatga gatcagcaaa
     7261 gccattaacg atgccgatag tggaatttcc gctagtatcg ttaaagtcaa agatggtaac
     7321 tatcaattgg tattgaccgc cagtgaaggt ctggccaata aaatgaccat atctgttgag
     7381 ggtgacagta aattaaatga tctgctggct tatgacagca agaccaacac cgggaatatg
     7441 aaggaactgg ttaacgcgca aaatgcacag ctaaacgtta acggtatcga tattgaacgt
     7501 tcaagtaaca aaattaccga tgctcctcag ggagtgactc tcgatttgac caaaaaagtc
     7561 actgacgtcc gggtcacggt gacaaaaagc aacgataaag caaccgaagc aattaaaggc
     7621 tgggtggatt catataactc cctcattgac acctttaaca ccctgacaaa atataaagaa
     7681 gtagacccag gtgctgaagc acaagataaa aataatggtg cacttctcgg agacagcgtt
     7741 gtacgtacaa ttcaaagcgg catccgcgca caatttgcaa acggtgccag cgacggtgcc
     7801 ttcaaaacat taaacgagat cgggattaag caggacggca cgacgggtaa gctgaaaatt
     7861 gatgatgata aactcaaaaa agtcctgaat gaaaatacag catccgtacg tgaattgctg
     7921 gtaggcgatg gtaaagaaac aggaattacc accaaaattg ccaccgaggt aaaaggttac
     7981 cttgccgatg atggcattat tgatagcgca caggacagta ttaacgccac actgaaaaaa
     8041 ctgaccaaac agtatctatc cgtcagtgcc agcattgatg acaccgtggc gcgttacact
     8101 gcacagttta cgcaattaga taccatgatg agcaaactga ataacaccag tacctatctc
     8161 agccaacaat ttactgcaat gagtaattcc tgatcacgga ggtcagcatg tataccgcga
     8221 gcggctcaaa agcctatgca caaattggcg tcgaaagcgc cgtaatgagc gccagccagc
     8281 agcagctggt caccatgcta tttgatggag tgctgagcgc actggttaga gcgcgcctgt
     8341 ttatgcagga caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca
     8401 ttgagaacgg acttcgggtg agtcttgatg aagagagcaa agacgaatta acccaaaact
     8461 tgattgctct ttatagctat atggtcaggc gcttgctgca agccaattta cgcaacgatg
     8521 tctccgcagt cgaagaagtg gaagcattaa tgcgtaatat tgccgatgcc tggaaagagt
     8581 cgttactctc cccttctttg attcaggacc cagtctgatg aaccatgcac cgcatttgta
     8641 tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga
     8701 acaatgggac gaactcatcg ccagcgaaat ggcgtatgtg aatgcggtgc aggagatagc
     8761 acacctgact gaagaggttg ccccgtccac cacgatgcag gagcagctcc gcccgatgct
     8821 gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc gtatggatga
     8881 actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg gtgttaagtg cctatggcga
     8941 tcagggcggc tttgtgctgg ctccgcaaga taacttctcg tgaatcttaa cgagctaatg
     9001 gttcgcgaat aatccgatta cggctacgct tctaatgttc cccttgaatg gagtcgaaga
     9061 atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg cggtaagctc
     9121 tggcctgaac tggccgagcg cgccgacggt tttaatgata ttggtatcaa tatggtctgg
     9181 ttgccgcccg cctataaagg cgcatcgggc gggtattcgg tcggctacga ctcctatgat
     9241 ttatttgatt taggcgagtt tgatcagaaa ggcagcatcc ctactaaata tggcgataaa
     9301 gcacaactgc tggccgccat tgacgccctg aaacgtaatg acattgcggt gctgttggac
     9361 gtggtagtca accacaaaat gggcgcggat gaaaaagaag ctattcgcgt gcagcgtgta
     9421 aatgctgatg accgtacgca aattgacgaa gaaatcattg agtgtgaagg ctggacgcgt
     9481 tacaccttcc ccgcgcgtgc cgggcaatac tcgaagttta tctgggattt caaatgtttt
     9541 agcggtatcg accatatcga aaaccctgat gaagatggca tatttaaaat tgttaacgac
     9601 tacaccggcg aaggctggaa cgatcaggtt gatgatgaat taggtaattt cgattatctg
     9661 atgggcgaga atatcgattt tcgcaatcat gccgtgacgg aagagattaa atactgggcg
     9721 cgctgggtga tggaacaaac gcaatgcgac ggttttcgtc ttgatgcggt caaacatatt
     9781 ccggcgtggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg
     9841 tttattgtgg cggagtactg gtcgcatgaa gtcgataaac tgcaaacgta tattgatcag
     9901 gtggaaggca aaaccatgct gttcgatgcg ccgctgcaga tgaaattcca tgaagcatcg
     9961 cgcatggggc gtgactacga catgacgcag attttcacgg gtacattagt ggaagccgat
    10021 cctttccacg ccgtgacgct cgttgccaat cacgacaccc aaccgttgca agccctcgaa
    10081 gcgccggtcg aaccgtggtt taaaccgctg gcatatgcct taattttgtt gcgggaaaat
    10141 ggcgttcctt cggtattcta tccggacctc tacggtgcgc attacgaaga tgtcggcggt
    10201 gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga gttaattctc
    10261 gcccgtcagc gtttcgccca cggtgtacag acgttatttt tcgaccatcc gaactgcatt
    10321 gcctttagcc gcagtggcac tgacgaatat cccggctgcg tggtggtcat gtcgaacggg
    10381 gatgatggcg aaaaaaccat taatctggga gagaattacg gcaataaaac ctggcgtgat
    10441 tttctgggga accggcaaga gagcgtagta accgacgaaa acggtgaagc gaccttcttt
    10501 tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaat tcatccccgg
    10561 cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt tatccagcgc
    10621 ggcggcacac tctgccgtcg ggcgatccac acgctccatc gtcataccat cgtactcaat
    10681 ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt tagtgagatc
    10741 atcagacatc aatgtcaact tgcccggcac ggcaatcacc cgctgccact gacggcaatc
    10801 cagggtatcg ccctctttag tgacaatcag gctgcctatc gcttccggac tgaccagcgc
    10861 acgttgcggc cctttggtct gccagtagcc cgccagccag tccggcgcgg gggttttcac
    10921 gacattgtta tagttttcga cttcggcaca gcccgccagc aacatgagcg cgcctgcaat
    10981 tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag aggattgtgg cattaaagcc
    11041 ctgatgtcgc cagcctttcc aggttcagag agaaatttga tctaactatt atttttgtga
    11101 attattaccc cgaaaataaa tttgcatgat gtatgattcg cgattcttgt ttcaaacctt
    11161 ctgagttcag aggctacatt catgtcatgg cagcaattca aacacgcctg gttgattaaa
    11221 ttctgggcac ccatccctgc ggtcatcgcg gcgggtattc tctccactta ctattttggc
    11281 attactggca ccttttgggc tgtcacgggt gaatttaccc gttggggcgg tcagctcctg
    11341 cagctgtttg gcgtccatgc tgaagagtgg ggttatttta aaattatcca tctggaagga
    11401 tcgccattaa cccgcatcga cgggatgatg atcctcggca tgtttggcgg ctgctttgcc
    11461 gcagcgctgt gggccaacaa tgtcaaactg cgcatgccac gcagccgtat ccgcattatg
    11521 caggccatta ttggcggtat tatcgctggt tttggcgcgc gtctggcaat gggctgtaac
    11581 ctggcggcgt tctttaccgg tattcctcag ttctcgctgc atgcctggtt ctttgccatc
    11641 gccactgcca ttggttcatg gtttggcgca cgctttaccc tgctgcccat cttccgtatt
    11701 cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga cgcaaaaacc ggatcaggcg
    11761 cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggta tgctgggctg ggcgctgctc
    11821 acggcgatga accaaccaaa actggggctg gcaatgctgt ttggcgtcgg ctttggttta
    11881 ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc gcgatatgtg gatcaccgga
    11941 cgtacccata tggcgaaagc aatcattatc ggtatggcgg tgagtgccat cgggatcttc
    12001 agttacgtac agttaggcgt tgaacccaaa atcatgtggg cgggaccaaa cgcggtaatt
    12061 ggtggtttac tgtttggttt tggcatcgtg ctggcaggcg gctgcgaaac cggttggatg
    12121 taccgcgcgg tagaaggtca ggtgcactac tggtgggtcg gtctgggcaa tgtgatcggc
    12181 tcaaccattc tggcgtatta ctgggatgat ttcgctccgg cgctggccac cgactgggac
    12241 aaaatcaacc tgctgaaaac ctttggcccg atgggtggcc tgctggtgac atatttgctg
    12301 ttgtttactg cgctgatgtt gattatcggc tgggaaaaac gcttcttccg ccgtgcggca
    12361 ccgcagactg ctaaggagat cgcatgaaaa atatcattcc tgattaccgt cttgatatgg
    12421 tgggtgaacc ctgcccttat ccggcagtcg caacccttga ggcgatgccg cagttaaaaa
    12481 aaggtgagat cctggaagtg gtgagcgact gtccgcagtc gatcaataat attccactgg
    12541 atgctcgtaa tcacggctat acggtgctgg atattcagca agacgggccg accattcgtt
    12601 atttaattca gaagtaatcc ctcattcccg ctgtggccaa cagcgggaat atcgttgcct
    12661 ctgtaccaca tagattttac ccgccccctc tcctattctt ttcagtgtgc tctcaggagg
    12721 ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga agattacctt gcgcttctcg
    12781 cggaagatat tgaacgcgat attccctatg atcccgaacc cattggcaga tacaacgtcg
    12841 cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat ctggatccgg
    12901 ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac gcccgcgtag
    12961 aaactgcggc caccagtcgt atgtttaaac cgctctggca acatggtcgg gcaatctgtt
    13021 ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaacagcct tattttatct
    13081 atcgtgctga cggacaacct gtttttatgg ccgcgatagg tagcacaccg tttgagcgcg
    13141 gtgacgaagc cgaaggattt ttaattgtca ctgcagcagc agaccagggt ctggtagata
    13201 ttcatgaccg ccgcccgctg gtactgtcgc cagaagctgc gcgggaatgg atgcggcaag
    13261 agattggcgg taaagaagca tcagaaattg ctaccaacgg ctgtgtgcca gcaaaccagt
    13321 tcacctggca cccggtatcg cgcgcggtag gtaatgtcaa aaaccagggc gcggagttaa
    13381 ttcaaccggt ttgataagcc aggagactat tgatcaataa taccgcgtca taagaaatct
    13441 ctattagaca aagtagacgc tccttccaca tgcgccacaa gaaacctcta ttccagtgac
    13501 acaattacgc ctgattattt ccctagcccg gaatatgaat aaaaacatca acaaagatta
    13561 aattattcaa acgttactgt tgcgtctgcg gtcgtaacaa catccgacgt ggtgatgtgt
    13621 atcaaatgag gcctctgatg atcagagggt ttattgcgta tacggatcgt ggatggcaga
    13681 aaactttcag gatcaggtat ccattttaaa ccagaaatag ggtgagtttg ccccatccat
    13741 gccccacgcg ggcagatggg atgtaattaa caacaggtta caaaacctac acttgcatgg
    13801 acatcacttc ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag
    13861 aaactgaggc tttttgcata tcggtcatca catcgtttaa cgccacgccg ggttcaccga
    13921 gggtgaattt ttctgcctgc gtgcgggcag ctgtttgtgt atcgctaatg cgatcgagcg
    13981 cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg cagtgaatcc tgcgcgcgcg
    14041 cactcatcgc cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca
    14101 ttttcatctc ctggataatt tctggtagca aagcctacca gtaagtcaat aagacaaagg
    14161 cgctaaatag caacaaaaaa acgggtttat tggcggatag aaaaaaacga aagcacaaat
    14221 aatgggagcg tcaatttttc gagtttgctg acccgggagt gagtcttgtt ccactttgcc
    14281 aataacgccg tccataatca gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa
    14341 aatctcttga gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttgccg
    14401 gttccgcggc agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc
    14461 gcacattatt cagcaatctt tccgatcagg atggtggcgc aattgtcagc caactgacgc
    14521 aaatgaatat tccttaccgc ttcagcgaag ccagcggcgc tattgaagtt ccggcagata
    14581 aagttcacga actgcgtctg cgcctggcac aacaaggttt gcccaaaggc ggcgcggtcg
    14641 gtttcgaact actcgatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt
    14701 atcagcgggc gctggaaggc gagctttctc gtaccatcgc aactatcggc ccggtaaaag
    14761 gggcgcgcgt acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc
    14821 cttctgcatc ggtgacggta aatctgttac ccggccgcgc actcgatgaa gggcaaatta
    14881 gcgccattgt gcatctggtt tccagcgccg ttgctggtct gccgccggga aacgtcacgc
    14941 tggtggatca gggcggacat ctgttaaccc agtccaatac cagcgggcgc gatcttaatg
    15001 atgctcagtt gaaatatgcc agcgatgtcg aaggccgtat tcagcggcgt attgaagcaa
    15061 tcctgtcgcc tattgttggt aacggtaata ttcacgccca ggtcacggcg cagctggact
    15121 tcgccagtaa agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg
    15181 cgcttcgttc acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg
    15241 taccgggggc gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc
    15301 cagcaaatca aaataaccgc cagcagcagg cgagcaccac cagcaatagc gggccgcgta
    15361 gcacacagcg gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa
    15421 tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc
    15481 cagacggtaa accattgcct ctcagcaacg aacagatgat gcaaattgaa gatctgaccc
    15541 gcgaggcgat gggcttttct gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt
    15601 tcaatagcag tgacgaaagc ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg
    15661 atcagttgct ggctgccggt cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc
    15721 ggaaagcggt acgcccgcag ttaacacgtc gcgccgaggc gatgaaagct gtacagcaac
    15781 aggcgcaggc ccgtgaggaa gtggaagatg cggtggaagt ccgcctgagc aaagacgaac
    15841 aactccaaca acgacgcgct aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc
    15901 gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata
    15961 acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc
    16021 gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc
    16081 gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag
    16141 tttgagcaag aagctgaaca gtttgccgca ctgaatatca acgccaacga ttatctgcgt
    16201 tcggtattgg tcaaagctct gggtgaggaa cgtgccgcca gcctgctgga agatattctc
    16261 gaaactcgcg ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagtgcc
    16321 gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgaag
    16381 cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg
    16441 ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta
    16501 ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg
    16561 gcagccgaaa ttatcaacct gatgaaaact cagcaggaag aagccgttat taccgccgtg
    16621 cgcgaattcg acggcgagct ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat
    16681 ctggtggatg tcgacgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg
    16741 ctgttgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg
    16801 tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg
    16861 tcgcaggtgg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact
    16921 ggcgagatgg taattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac
    16981 ctggacgccg gacgatctcg cgccaccaca ggcagagttt gtgcccatgg tcgagccgga
    17041 agaaaccatc attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat
    17101 gcaggcccat gagcaaggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcatga
    17161 gcagggctat caggaaggac tggcccgggg gctggagcaa ggtctggcag aggcgaagtc
    17221 tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct
    17281 tgatgcgctt gatagtgtga ttgcttcgcg cctgatgcag atggcgctgg aggcggcacg
    17341 tcaggtcatc ggtcagacgc caacagtgga taactcggca ctgatcaaac agatccaaca
    17401 gttgttgcag caagaaccgt tattcagcgg taaaccacag ctgcgcgtgc acccggatga
    17461 tctgcaacgt gtggatgata tgctcggcgc aaccttaagt ttgcatggct ggcgcttgcg
    17521 gggcgatccc acactccatc ctggcggctg taaagtctcc gccgatgaag gcgatctcga
    17581 cgccagtgtc gccactcgct ggcaagaact ctgccgtctg gcagcaccag gagtggtgta
    17641 atgaccacgc gcctgactcg ctggctaacc acgctggata actttgaagc caaaatggcg
    17701 cagttgcctg cggtacgtcg ctatgggcga ttaacccgcg ctaccgggct ggtgctggaa
    17761 gccaccggat tacaattgcc gctcggcgca acctgtgtca ttgaacgcca gaacggcagc
    17821 gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg
    17881 ccgctggagg aagtcgaagg tgtcctgccc ggcgcgcggg tttatgccaa aaacatttcg
    17941 gctgaaggac tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt aggtcgcgtt
    18001 ctggacggca gcggtaagcc gctcgatggc ctgccctccc ccgatacgac ggaaaccggt
    18061 gcgctgatta ccccgccatt taccccgttg caacgtacac cgattgaaca tgtgctggac
    18121 accggcgtgc gcccaatcaa tgccctgctt accgttgggc gtgggcagcg tatgggactg
    18181 tttgccgggt ccggcgttgg taaaagtgtg ctgctgggga tgatggcacg ttacacccgc
    18241 gccgatgtca ttgtcgtggg tttgattggt gaacgcggac gcgaagtaaa agattttatt
    18301 gagaatatcc tcggtgccga agggcgtgca cgctccgtgg tgattgccgc tccggcggat
    18361 gtttctcctc tcctgcgaat gcagggtgcc gcctatgcca cgcgcattgc cgaagatttt
    18421 cgcgatcgtg gtcagcatgt gttgctgatt atggactccc tcacccgcta cgcgatggcc
    18481 cagcgtgaaa ttgcgctggc gattggcgaa ccacccgcca ctaaaggtta tcccccgtcg
    18541 gtgtttgcca aattaccggc actggtcgag cgtgccggaa atggcattag cggcggcgga
    18601 tcgattactg cattttatac cgtgctcact gaaggcgatg accagcagga ccccattgcc
    18661 gactctgcgc gggcaatcct cgacggtcac attgtgctgt ctcgccgact ggcggaagcc
    18721 gggcactatc cggctatcga tattgaagcg tcgatcagcc gcgcaatgac ggcgttgatc
    18781 agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt
    18841 aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca gcgatccgat gctcgacaaa
    18901 gccatcgccc tgtggccgca gctggagggc tatttgcaac aaggcatttt tgaacgcgcg
    18961 gactgggaag cgtctctcca ggggctggag cgtattttcc cgacagtgtc ataacccagg
    19021 agataacggc agatggcaga acatggcgcg ctggcgaccc tgaaagatct ggcagaaaaa
    19081 gaggtagagg atgccgcgcg cctgctgggt gaaatgcgtc gcgggtgtca gcaggcggaa
    19141 gaacagctca aaatgctgat cgattatcag aatgaatatc gcaataacct caacagcgat
    19201 atgagtgccg ggatgaccag caaccgctgg atcaactatc agcagtttat ccagacgctg
    19261 gaaaaagcca ttactcagca tcgccagcaa cttaatcagt ggacgcagaa agttgacatt
    19321 gccctgaaca gctggcgaga aaagaaacaa cgtttgcagg cctggcagac actgcaggaa
    19381 cggcaatcca cggcggcact gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag
    19441 ttcgcccagc gcgccgccat gaggaaacct gaatgattcg cttagcgccc ttaattaccg
    19501 ccgacgttga caccaccaca ttgcctggcg gcaaagccag cgatgctgca caagattttc
    19561 tcgcgttgtt gagcgaagca ttagcaggcg agacaactac cgacaaagcg gccccccagt
    19621 tgctggtggc aacagataag cccacgacaa aaggcgagct gctgatcagc gatattgttt
    19681 ccgacgcgca acaagcggat ttactgatcc ctgtggatga aacactgcct gtcatcaacg
    19741 tcgaacaatc cacatcaaca ccgttaacca ccgctcagac gatgacgttg gctgcggtgg
    19801 ctgacaaaaa tacgacaaaa gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa
    19861 gcctgagcgc cctttttgcg atgttgccgg gttttgacaa tacgcccaaa gtgactgatg
    19921 tgccgtcaac cgtgttaccg gcagagaaac caacgctctt cacaaaactg acttctgcgc
    19981 aactcacaac agcacagcct gatgatgccc ccggcacgcc agctcagcca ttaacaccgc
    20041 tggtagcaga agcccagagt aaagcggaag tcatcagcac accgtcaccg gtgaccgctg
    20101 ccgccagccc gctaattact ccacaccaga cacagccact gcccaccgtc gccgcgcctg
    20161 ttttgagtgc accgctgggt tctcacgaat ggcaacaatc attaagccag catatttcgc
    20221 tattcacccg ccaggggcaa caaagtgcag agttgcgtct gcacccgcag gatttaggtg
    20281 aagtgcaaat ctccctcaaa gtggatgata accaggcaca aatccagatg gtttcaccgc
    20341 atcagcatgt acgcgccgcg ctggaagcag cgcttccggt actgcgtacg cagctggccg
    20401 aaagtggcat tcagttaggg caaagcaaca tcagtggcga aagctttagt ggtcagcagc
    20461 aggccgcttc ccaacaacag caaagccaac gcacagtaaa ccatgaacct ctggcggggg
    20521 aagacgacga tacgcttccg gttcccgtct ctatacaagg gcgcgtaaca ggcaacagcg
    20581 gcgttgatat tttcgcctaa cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt
    20641 tgttgcgctc aagacgcagg ataattagcc gataagcagt agcgacacag gaagaccgca
    20701 acacatgact gattacgcga taagcaagaa aagcaagcga tcgctttgga tcccgattct
    20761 ggtattcatt accctcgcgg cctgtgccag cgcaggttac agctactggc attcgcatca
    20821 ggttgccgct gacgacaaag cgcagcaacg cgtcgtaccc tccccggtct tctacgcgct
    20881 ggataccttc acggtcaatc tgggcgatgc ggatcgcgtg ctttatatcg gcgtaaccct
    20941 gcgcctgaaa gatgaagcta cccgctcgcg gctgagtgag tatttgccgg aagtccgtag
    21001 ccgtttgctg ttactgtttt cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa
    21061 gaaaaacctg attgccgaga ttaaaaccac gctttccacc ccgcttgttg ccgggcaacc
    21121 gaaacaggat gtcaccgacg tgctgtatac cgcttttatt ctgcgataac gacatgggcg
    21181 atagtattct ttctcaagct gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag
    21241 acgaaccgac agccagtgtt agcggcgaaa gtgacattcg tccgtacgat ccgaataccc
    21301 aacgacgggt tgtgcgcgaa cgtttgcagg cgctggaaat cattaatgag cgctttgccc
    21361 gccattttcg tatggggctg ttcaacctgc tgcgccgtag cccggatata accgtcgggg
    21421 ccatccgcat tcagccgtac catgaatttg cccgcaacct gccggtgccg accaacctga
    21481 accttatcca tctgaaaccg ctgcgcggca ctgggctggt ggtgttctca ccgagtctgg
    21541 tgtttatcgc cgtggataac ctgtttggcg gcgatggacg cttcccgacc aaagtggaag
    21601 gtcgcgagtt tacccatacc gaacagcgcg tcatcaaccg aatgttgaaa ctggcgcttg
    21661 aaggctatag cgacgcatgg aaggcgatta atccgctgga agttgagtac gtgcgttcgg
    21721 aaatgcaggt gaaatttacc aatatcacca cctcgccgaa cgacattgtg gttaacacgc
    21781 cgttccatgt ggagattggc aacctgaccg gcgaatttaa tatctgcctg ccattcagca
    21841 tgatcgagcc gctacgggaa ttgttggtta acccgccgct ggaaaactcg cgtaatgaag
    21901 atcagaactg gcgcgataac ctggtgcgcc aggtgcagca ttcacagctg gagctggtcg
    21961 ccaactttgc cgatatctcg ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg
    22021 tcctgccgat agaaaaaccc gatcgcatca tcgcccatgt tgacggcgtc ccggtgctga
    22081 ccagtcagta cggcactctc aacggtcaat atgcgttacg gatagaacat ttgattaacc
    22141 cgattttaaa ttctctgaac gaggaacagc ccaaatgagt gacatgaata atccggccga
    22201 tgacaacaac ggcgcaatgg acgatctgtg ggctgaagcg ttgagcgaac aaaaatcaac
    22261 cagcagcaaa agcgctgccg acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg
    22321 aacgttgcag gatatcgacc tgataatgga tattccggtc aagctgaccg tcgagctggg
    22381 ccgcacacga atgaccatca aagagctgtt gcgtctgacg caagggtccg tcgtggcgct
    22441 ggacggtctg gcgggcgaac cactggatat tctgatcaac ggttatttaa ttgcccaggg
    22501 cgaagtggtg gtcgttgccg ataaatatgg cgtgcggatc accgatatca ttactccgtc
    22561 tgagcgaatg cgccgcctga gccgttagtg atgaataacc acgctactgt gcaatcttcc
    22621 gcgccggttt ctgctgcgcc actgctgcag gtgagcggcg cactcatcgc cattattgcc
    22681 ctgatcctcg ctgctgcctg gctggtaaaa cggttgggat ttgtccctaa acgcactggc
    22741 gttaacggtc tgaaaattag cgccagtgct tcgctgggcg cgcgtgaacg ggttgtggtg
    22801 gtcgatgtgg aagatgcacg gctggtgctc ggcgttaccg cagggcaaat caatctgctg
    22861 cataaacttc ccccttcagc accaacggaa gagataccgc agcccgattt tcagtcggtc
    22921 atgaaaaact tgcttaagcg tagcgggaga tcctgatgcg tcgtttgttg tctgtcgccc
    22981 ctgtccttct ctggctgatt acgcccctcg ccttcgcgca actgccgggt atcaccagcc
    23041 agccgctgcc tggcggtgga caaagctggt cgctcccggt gcagacgctg gtgttcatca
    23101 cctcgttgac gtttattccg gcaattttac tgatgatgac cagtttcacc cgcatcatta
    23161 ttgtttttgg tttattgcgt aacgcgctgg gaacaccctc cgcgccacct aaccaggtat
    23221 tgctggggtt ggcgctgttt ttgacctttt ttattatgtc gccggtgatc gacaaaattt
    23281 atgtcgatgc gtaccagcca ttcagcgaag agaaaatatc aatgcaggag gcgctggaaa
    23341 aaggggcgca gccgctgcgt gagtttatgc tgcgtcagac ccgtgaggcg gatttagggt
    23401 tgtttgccag actggcgaat actggcccgt tgcagggacc tgaagccgtg ccgatgcgca
    23461 ttttgctccc ggcctacgtg accagcgagt tgaaaaccgc atttcagata ggcttcacga
    23521 ttttcatccc ttttttgatt atcgacctgg tgattgccag cgtgttgatg gcattgggga
    23581 tgatgatggt cccccccgcc accattgctc tgccctttaa actgatgctg tttgtactgg
    23641 tggatggctg gcaattgctg gtcggttcgc tggcgcagag cttttacagc tagagaggca
    23701 aaatgacacc tgaatcggtc atgatgatgg ggactgaagc gatgaaagtc gcgctggcac
    23761 tggctgcccc gctgttgctg gtggcgctgg tcacgggcct tatcatcagt attttgcagg
    23821 ccgccacgca gatcaacgaa atgacgctgt cgtttattcc aaaaatcatc gccgtattta
    23881 ttgccattat tattgccgga ccgtggatgc tcaatctgtt gctggattac gtccgcacct
    23941 tgttcactaa cctgccgtat atcatcgggt agcccgtaca atgatgcagg tgacaagcga
    24001 tcaatggctt tcctggctaa gtctgtactt ctggccgtta ctgcgcgtgc tggcgctcat
    24061 ttctaccgcg ccaattctga gcgaacgcag cgtgccgaaa cgggtaaaac tgggtctggc
    24121 aatgatgatc acgttcgcta ttgccccatc attacctgcc aacgatgttc ctgttttttc
    24181 gttctttgct ctgtggctgg ccgtgcagca gatcctgatc ggcattgcgc ttggtttcac
    24241 catgcaattt gcctttgccg ctgtgcgaac cgctggcgaa attatcggtc tgcaaatggg
    24301 gctgtcattt gcgacgtttg tcgatccggc cagccatctc aacatgcccg ttctagcgcg
    24361 tatcatggat atgctggcgt tactgctgtt cctgacattt aacggtcatt tatggttgat
    24421 ttcactgctg gtcgatacct ttcacaccct gccgattggt ggcgaaccgt tgaacagcaa
    24481 tgcgtttctg gcactcacca aagcagggag tttgattttc cttaacgggc tgatgctggc
    24541 gttaccgctc attactctgc tgctgaccct gaatctggca ttaggtttac tgaatcgtat
    24601 ggccccgcaa ttatccattt ttgttattgg atttccatta actctgactg tcggtatctc
    24661 tttaatggcg gcattaatgc cgttaattgc gcctttttgc gaacatttat tcagtgagat
    24721 gtttaatttg ctggctgata ttattagtga attgccatta atataattcc gtaacgttta
    24781 tcatgttatc ctaaggatta tccgaaaaat aatacctacg aacatcttcc aggatactcc
    24841 tgcggcgaaa tatttgtttt aagctcactc acatatcgca acatttactt taagacaatt
    24901 ccaggcaaat tatacaacac tttacgggat agtaagtccg cctgaaaaat cgcgagagtg
    24961 gcgcattagg tgacccatgt tgttccgttt agtcatgatg aaatattcag gtaaggggaa
    25021 ttatcgttac gcattgagtg agggtatgcc atgtcaacga ttattatgga tttatgtagt
    25081 tacacccgac taggtttaac cggatatctg ttgagtagag gggttaaaaa aagagaaatc
    25141 aacgacattg aaaccgttga tgaccttgcc atagcttgtg attcacagcg cccttcagtg
    25201 gtgtttatta atgaggactg tttcatccat gatgcttcta acagtcagca catcaagcac
    25261 atcattaatc aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat
    25321 tttgatgaat atctattggt cagaaaaaat ttattgataa gttctaaatc gattaaaccg
    25381 gaatctctcg acgatatcct tggcgatatt ctgaaaaaag agacaacgat aacctctttt
    25441 ttaaatatgc cgacgttatc attgagccga accgaatcga gtatgttgcg aatgtggatg
    25501 gcaggtcagg gaacgattca aatctctgac caaatgaata tcaaagccaa gaccgtttca
    25561 tcgcataaag gtaatattaa acgtaagatc aaaacgcata ataaacaggt tatctaccat
    25621 gtcgtccgac tgacggataa tgtgactaat ggtatttttg tcaacatgcg ctaacacatt
    25681 ctgactggtg gtttcccacc agtcaggctg aataagatta ctctgctttc tccacaaaga
    25741 taccgtcctg atgccctgct tcattaaaga accagatgcc gagcgggtag tcttccagcg
    25801 aaaccaggta cattgtgcct tcactaaact cctcaactgc cagtaccacg ccaggacgac
    25861 gcggaccgcc atccgttttg actgttaccc gatcattcac cttcatcgtt ttcctcctgt
    25921 ggctttgtgc cagtgtagaa caatttcgtt tttctggcag cgccaggcgc gcgcgagtgc
    25981 tgattttctc gacggtctat acttaagaga tgccaggcgg acttaacgac tggcggcaac
    26041 aacagagtaa cggttgcgag gaaagatgat gaaaaccgca aaagagtaca gcgataccgc
    26101 aaaacgtgag gtcagcgtcg atgtcgatgc cctgctggcg gcgatcaatg aaattagcga
    26161 aagcgaagtt catcgcagcc agaacgattc tgaacacgtt agcgtcgatg gacgtgaata
    26221 tcatacatgg cgtgaattgg cggatgcctt cgaactggat attcatgact tcagcgtctc
    26281 tgaagtgaat cgttgaatgc acaataaaaa aatcccgacc ctgagggggt cgggatgaaa
    26341 cttgcttaag caagaagcac ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc
    26401 accttatccg caattttttt cgctgacaag aaaatattcg cgatatgaat gattaacgct
    26461 acttatgaat agaaatgtga cgtgcgtcac cccttggtgg ctctggcgtc aacgggatcg
    26521 ctcccgaaaa aatcatcttt gattatttgt gctgttttga gaatcatcct ggtaagggga
    26581 gaactgcgcg accatgtttt caattcaaca accactactg gtttttagcg atcttgatgg
    26641 caccctgctg gactgtcata gttatgactg gcaaccagct gccccctggc tcagccgttt
    26701 acgcgaagcg aatgttcccg tcattctctg tagcagtaag acatcagcgg aaatgttgta
    26761 cttgcaaaaa acgttggggc tacaaggttt accgctgatt gccgaaaatg gcgcagtgat
    26821 ccagcttgct gagcaatggc aggatataga cggttttcca cgcatcatct caggtattag
    26881 ccatggcgaa atcagccagg tattaaatac gctacgcgag aaggaacatt ttaaattcac
    26941 gacttttgat gatgtcgacg atgcaaccat cgccgaatgg acgggattaa gccgtagcca
    27001 ggcggcgctg acgcagctgc atgaggcgtc ggtaacgcta atctggcgcg acagtgacga
    27061 gcgtatggca caatttaccg ctcgtctgaa cgaactgggc ttacagttta tgcagggtgc
    27121 gcgcttctgg cacgtcctgg atgcctctgc cggaaaagat caggccgcca actggattat
    27181 cgcgacctat caacaattat caggcaaacg cccaaccaca cttggcctgg gcgatgggcc
    27241 aaacgatgcg cccttactgg aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg
    27301 tgaaggggtg catctgcatg atgaggatcc gacccgcgtc tggcgaacgc agcgtgaagg
    27361 accggaaggc tggcgtgaag ggctggacca ttttttctcc gctcgttaag cgttatcgct
    27421 cgcgcatacc cgattacgcc cggcctgttt agcgagataa agccgacggt cagccagtga
    27481 ctggagttgt tcaaaatcat aatcaccggt ttcctcgctg ctactaaccc ccaacgaggc
    27541 actgatgcgt atcgtcgtac tcttagcaat caacatctct ttttcattta acttcaggcg
    27601 aatacgttct gcgacttccg cagcctccgt cagactcgcg cctggcagaa tcacacaaaa
    27661 ctcctcacca ccgacccgcc cggcaacgtc ctgcgcacgc aaggaactgc taattaatcc
    27721 ggcagcatga gaaagaacac ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt
    27781 aaaatggtca agatcgacct gaatgacaga aaaaggatgt tggtgcgtct gacacaattt
    27841 agcgagcgga cgggcttttt cgaacagtgc gccacgatta tataaacggg ttaaggtgtc
    27901 gtgccacgcc tgccactgca acgagctttg cagaacatac atgttgctga ccatccggcg
    27961 aatcacatac caggagatga gtaacatggt ggtaaagagc gcccacagca gggttaatgc
    28021 aatgctgata ctaccgaaat cgccgcgcac gccttcgctt agcgtatgga cgcgcaccag
    28081 cacaccgtcg aaatggtcca gacgttccca gctaacatag cgactgtcca tacgaatgcc
    28141 gccccgcgtg tcatgttcca tcgcctgcgc cagcaaggcc agttcacgag gatcaaaaat
    28201 attccctgtt ggatggtcag gattggaaga ggtcaaaaat ctcagcttac tgtcatagag
    28261 ctgatactca ccatcgaggt ttttatcgat ggcgtttctt aaaaattgct gcatagtacg
    28321 cacaggaata ctcatcccca gcacgccata ccagtagtta ttactgtcta ccggaacact
    28381 gacggtaacc tgcggttcag tattgctggc gtgttccggt tgcgaagtaa accagcgtac
    28441 cgcgcggtga cgattttctc gttgcgaatg gccgataaac cagggttggg tgacatagcc
    28501 gtaataacgc gttggaacat tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc
    28561 ggcacgcgag acatacatcg cctgttcaac catcgacgag gagttgtgcg ccagtcgcag
    28621 caagtaacca acttccagtg cagcggtaat ttcattgtcg aggctttcat tttcgcgaga
    28681 caggagattc ccctcgctga ctaatgcatc cgacacacca ttaaatggca gagtgcgccg
    28741 tcggttgagt tcaatttgcc aggcgtgctc gtcgcgatgc tgttcgaact cggttacagc
    28801 attacgcaga gaggtgaaat ccagtggcgc tacgagagct tcgcgcatgc cattacgcaa
    28861 aaagatcagt ttgtcgacgt tatactgcaa atgcttatcg agcgcgttgg ctacgttttc
    28921 cagatgatta cgctggctgg agatataggc atcttccagc accactactt cacgccaggt
    28981 gagcaaggtg gaaaaaagca ataccacgat aaagcagaga ttaacgacat gaccaggccc
    29041 caggcggcgt gcgagttttt tcaaccagct ctggttttcc atttttgtct cgtgctgcac
    29101 cctgacgact cccaacctgc ttttttatga ttctggcata acctggcgat agcgtaccgc
    29161 aaaacagcga taattttata atggtggcat gataagacgc gactatcatt cattgcaggc
    29221 attggcacac caggccggat gtgatccggc ctggtgttaa tgataccctt tcaaacttct
    29281 tccgcagaac gactcccctc ccccggaaca agctcggttt catgaaaagc ttcccgcttg
    29341 accccgtaac cgtcatacca acgacactca accataccgc tggagtatcc ggtgacaatc
    29401 atccgcgggc caccctcttt aaccgtaact tcctcactaa ccataaagct catactcgcc
    29461 tccttttttc gagtgaaact tgttcacctt agttgaagat ggcgaatttt gcataatagc
    29521 ctgtgcgagt attaatgtgc ctgaccgcgc attttcgcta cggctttcac tcccagcacc
    29581 acgatgccgc cgatgataaa tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc
    29641 accactgcac tttgctgccc ggcgaaatgt tcaatggcgt gatgcagcgg cgcaatacca
    29701 tgtaccacaa tcccgccgcc gacgaggaac atcgccagcg tgccgacaat cgataacgcc
    29761 ttcatcaacc agggcgcaat gatcaataac cctttaccta atgcctgcat cagcgcgctg
    29821 gatttttccg ccagccaata ccccaggtca tcaatcttaa cgataacccc taccagaccg
    29881 tagacgccca cggtcactac cagcgcgatg cctgaaagca ccagcacctg attaagcaac
    29941 ggcgcttcgg ccacaatccc cagcgtgatg gcgacgattt cggcagacaa tataaaatcg
    30001 gtacgaatcg ccccttttat tttgtccttt tcaaacttca gcggatcctg cgccgccagc
    30061 ttctccagac gctgctggct ctgcgccgga tcttctttat gtttacgcgc ctccagcata
    30121 tgcagcactt tctctactcc ttcaaagcag agaaacgcgc caccaatcat caacagaggc
    30181 gtaatcgccc acgggataaa cgcactgatg atcagcgcca gcggcaccag aatcacttta
    30241 ttaatcagcg atcctttcgc cacgccccag accacgggaa gttcccggtt ggcccgcacg
    30301 cctgaaactt gttgcgcatt gagcgataag tcatccccta atacaccggc ggttttcttc
    30361 gccgccagtt tgcccatcac ggagatatcg tccagtagtg tggcgatatc atcgagcaac
    30421 gtcagtaaac tactcccggc caaaagcatt ccctctcatt tttattgttg aagggtgtaa
    30481 gtatgaagca aaaacggcag ctctgatact tgcttcacag gtcaacattt ttttaacaat
    30541 taagtgcaat tatatctctc gccagcagaa tgattttcac gtttactatg aaccaccttt
    30601 ttatttccac cgtgagggat tatgcgtttc cgccagttgt taccgctttt tggcgcgctg
    30661 tttgcgttgt atatcatttg gggctcaacc tattttgtca ttcggattgg cgtggaaagc
    30721 tggcctccgt taatgatggc aggcgttcga ttcctggcag ccggtatttt attgatggca
    30781 tttttgctac tgcgcggaca caagctcccc ccgctacgtc cgctgctcaa tgccgcgctg
    30841 attggcctgt tattgctggc tgtcggtaat ggcatggtga cggttgccga gcatcaaaat
    30901 gttccttccg gcatcgccgc cgttgtggtt gcaaccgtgc ccctctttac cctgtgcttc
    30961 agccgcctgt ttggcattaa aacgcgcaaa ctggaatggg tgggtatcgc cattgggctt
    31021 gccggaatca tcatgctcaa tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt
    31081 ctgattttaa tcggctcgat tagctgggcg tttggctcgg tttatggctc gcgcattacc
    31141 ttacctgtag ggatgatggc gggtgctatt gagatgctgg cggcaggcgt ggtgttaatg
    31201 atcgcgtcga tgattgcagg tgaaaaactg acggcgctcc cttccctttc aggcttcctt
    31261 gcggtcggct atctggcgct gtttggttcg attatcgcca tcaacgctta tatgtattta
    31321 atccgtaatg tcagtccggc tctcgccacc agctacgcct acgttaaccc ggtggtcgcg
    31381 gtcttgctgg gtacgggact gggcggagag acgctgtcga agattgaatg gctggcgctc
    31441 ggcgtaattg tcttcgcggt ggtgctggtc acgttgggaa aatatctctt tccggcaaaa
    31501 cccgtagttg cgccagttat tcaggacgca tcaagcgagt aaatgaatcc cctgcgtgtc
    31561 gatctgcgcg ctggcacctt cgccgcagat ccactcttcc agacgctcgc taagcgcttc
    31621 atccgtcagc ttctcgcgcc cacgtaacgc gcactcccag acaatcaata cgcgccagcc
    31681 gagttcctgc aagcgactga tatcgcggcg atcgcgctca acatttttac ctatcttctc
    31741 cagccagaac tcggttcgag tcgcaggcac tttaaacaga tagcagtgat gatgatgcca
    31801 gaagcagccg tgggtaaata tcacgcagcg atattcatca acgacaaaat ccgggcgtcc
    31861 gggcagactg gcgtcctgaa cgcgaaacgc caggccctgc ccggttaaca gactggcgag
    31921 gcgcttctct atcgccgtat cacgcgtggc aatcgcgcgc atatttttgc tgcgagtggc
    31981 cttatcgtga acgtcggcca tgttgtgcct cttgctgacg caacgccacc gcctgtttga
    32041 tttttggctc aagcagtttt gccaccgcgg caaagaccgg cacgaccacc gagttaccga
    32101 actggcgata ggcctgagtg tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt
    32161 caaaacccat taagcgcgcg cattcacgag gcgttaaccg acgtggacga tgttgctgat
    32221 tctgcggatc gtcaaagtct ttctcacctt tggccatatc ccagccgcga tcgattaaaa
    32281 tttccgcgcc atctttgtaa taacgcgcag acagcgtacg cgtgacgctt tgcggattgt
    32341 tcggataaac cattccataa ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc
    32401 gatagaggta cttccacagc accggcgtca ggatatattt cgcctcgacc atcgggtcca
    32461 gcagctgcgc cagcgtcact cgctgcgcag ggaaacattc gctgatatca cgcagggtaa
    32521 aatcggcttt cagattcaga tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct
    32581 gtggcagaaa atgtttgccg tcgatgattt tcggatcgtc tggcccgtta tcttctgcat
    32641 cagccacgtc atagcccagt tcgtccagcg tctgcatgat gatgcggaac gttttaccct
    32701 ggtcatggct tttcaggttt ttgacgtttt cgagcacaaa catcgccgga cgacgcgagt
    32761 cgataatgcg caccacatca aaaaacagcg tgccctgggt atcgcaggca aaaccgtgcg
    32821 cccgcccgag cgagttcttt ttcgatacgc cagccagcga aaatggctga caagggaaac
    32881 cggccagtaa aacatcgtgt tcaggaatgt gttgacgaat atgttccgcc gccgcctcat
    32941 cactcacgcc ttctttatgg ctgagggtga tgtcgcggat atcttcatta aaatgatgcg
    33001 tcgccgggtc gcaataatga ttggctttat aagtgcgtac cgcatgtttg ttccattcgc
    33061 tggtaaacac gcactgtccg ccaatcgatt caaaaccgcg acggatgccg ccaattccgg
    33121 caaatagatc gataaagcga aacgcataat gcggatgatg tgccggtggt ttgggtaaca
    33181 acgtttgcag atgggcgaac tctttctcac ttaaacggtg ccatgccgag tcattcgcca
    33241 gcgcacgttt taaaattgcc gcgctccagt gattctcacc cacaccatta agctgcgcca
    33301 ccagcgtttt aacatcataa atttgcagca gtttctccag cattgcctgt gcggcattcc
    33361 cggtgctgta tgaatcggtt actgatatat tttcctgcat agatttaacc ggccatctga
    33421 gatggccgac aggttaacat aattacagcc atttagggcc acgcgcttat cccgcgggtg
    33481 aaaacgcatc tatcaccttg tgatcgacac cttcattctc ccccgccagt tcggcactga
    33541 gcttcgccat aaactcgact aaaaaatgcg cgttgtgctg cgccagctgc ttgcctcttg
    33601 cggtttgcat ggtttgcggt agcttcagca acttagtctg gaaatgatcc agcgcgtagc
    33661 gtttatcatc aagcgggcga tgctgtgcga acggatcttc accatcaaac agcgccacgc
    33721 ccaatgcccc tgaaacggca aacacgcgcg ccaggccaat cgcccccagg gcttccagcc
    33781 gatccgcatc ctggacgatt ttagcttccg ttgttaaggg ggcgatttgc gcgctgaaac
    33841 tgtgagcggc aatggcatga caaacggcct cgattttctc cgccggaaat tgctcgaact
    33901 cttcacggag caggcgacgc gtctcttctg ccgccaggat tgaagaacgc tgcctttgcg
    33961 gataattttt cgccaggctc acaatatcgt gaaaataaca ggcggttaaa atcaccagca
    34021 tatcgacatc gtcatctgcc gccagcttct gtgccgtagc ccagacgcgg cgaaaatgac
    34081 acacgtcatg ggccgcgtcc tggtgctgat gatggttttt caaccagttt tcgaactgtg
    34141 cctgccagtg ttgtaagtcc atacgcgctc cgttgccaaa gagtcgccac cttagcagtt
    34201 tttattcata gcgtctgcta cgtgcgggac gaggtcgggt gtaccaggca atgctgccta
    34261 acaatgcccc gaccagagcc ataaataaaa atccgaccag tgcccctaac cactttcccg
    34321 cggtagagcc taaatcgctt tcaaatcccg acaccggggc atcggggatc cggctcatca
    34381 cccagatgta acgcgccatt gcccagacga aatagccaca ccatgtatag aaagcccaca
    34441 aagccagttt gccaccaggg ctgcgagaga gtctctcttc catctttgtc gaaccgtatt
    34501 ccattttgag ataacccgga aaattcagac ttagcactat tatgtgataa aagtcacatt
    34561 tttccacatt ggatgagcca gtcaagactc actcattttt tgaacaaaaa gataattttt
    34621 gcgcaacggc agtgaataca ggaaaaatcg ttttgagcga ttttatttat ctttgagatc
    34681 ggctttatta attcatttaa tcaatatatt agcactgaat acaattatac ctttttattt
    34741 ttctgtgtca tgatgcctcc gttattagcc ttttatcgtc ttgtttatat tttttgggcc
    34801 ggcatgatgc cggctttttt ttatgccttc attaatgtgc gcgtgatcac accagccgtt
    34861 tggcgcaaca atcattgata ccccctatgc ttccgctca
//
LOCUS       DAZFYC010000040        34292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFYC010000040 DAZFYC010000000
VERSION     DAZFYC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.040"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..761
                     /locus_tag="ACHKW5_002180"
                     /pseudo
     CDS             3..761
                     /locus_tag="ACHKW5_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(765..2156)
                     /gene="tcyP"
                     /locus_tag="ACHKW5_002181"
     CDS             complement(765..2156)
                     /gene="tcyP"
                     /locus_tag="ACHKW5_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO7511576.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2289..2879)
                     /gene="ydjM"
                     /locus_tag="ACHKW5_002182"
     CDS             complement(2289..2879)
                     /gene="ydjM"
                     /locus_tag="ACHKW5_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7511577.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3042..3710)
                     /gene="hxpB"
                     /locus_tag="ACHKW5_002183"
     CDS             complement(3042..3710)
                     /gene="hxpB"
                     /locus_tag="ACHKW5_002183"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO7511578.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3857..4393
                     /gene="yniB"
                     /locus_tag="ACHKW5_002184"
     CDS             3857..4393
                     /gene="yniB"
                     /locus_tag="ACHKW5_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO7511579.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4434..5294)
                     /gene="yniA"
                     /locus_tag="ACHKW5_002185"
     CDS             complement(4434..5294)
                     /gene="yniA"
                     /locus_tag="ACHKW5_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO7511580.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5400..5690)
                     /gene="ghoS"
                     /locus_tag="ACHKW5_002186"
     CDS             complement(5400..5690)
                     /gene="ghoS"
                     /locus_tag="ACHKW5_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7511581.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5791..6720)
                     /gene="pfkB"
                     /locus_tag="ACHKW5_002187"
     CDS             complement(5791..6720)
                     /gene="pfkB"
                     /locus_tag="ACHKW5_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO7511582.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7007..7765
                     /gene="ydiY"
                     /locus_tag="ACHKW5_002188"
     CDS             7007..7765
                     /gene="ydiY"
                     /locus_tag="ACHKW5_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO7511583.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7818..7925)
                     /gene="yniD"
                     /locus_tag="ACHKW5_002189"
     CDS             complement(7818..7925)
                     /gene="yniD"
                     /locus_tag="ACHKW5_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO7511584.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(8101..9999)
                     /gene="espL1"
                     /locus_tag="ACHKW5_002190"
                     /pseudo
     CDS             complement(8101..9999)
                     /gene="espL1"
                     /locus_tag="ACHKW5_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10523..12451
                     /gene="thrS"
                     /locus_tag="ACHKW5_002191"
     CDS             10523..12451
                     /gene="thrS"
                     /locus_tag="ACHKW5_002191"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO7511585.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12455..12997
                     /gene="infC"
                     /locus_tag="ACHKW5_002192"
     CDS             12455..12997
                     /gene="infC"
                     /locus_tag="ACHKW5_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO7511586.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            13094..13291
                     /gene="rpmI"
                     /locus_tag="ACHKW5_002193"
     CDS             13094..13291
                     /gene="rpmI"
                     /locus_tag="ACHKW5_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO7511587.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            13344..13700
                     /gene="rplT"
                     /locus_tag="ACHKW5_002194"
     CDS             13344..13700
                     /gene="rplT"
                     /locus_tag="ACHKW5_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO7511588.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13817..13941
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13823..13867
                     /gene="pheM"
                     /locus_tag="ACHKW5_002195"
     CDS             13823..13867
                     /gene="pheM"
                     /locus_tag="ACHKW5_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO7511589.1"
                     /translation="MNAAIFRFFFYFST"
     gene            14151..15134
                     /gene="pheS"
                     /locus_tag="ACHKW5_002196"
     CDS             14151..15134
                     /gene="pheS"
                     /locus_tag="ACHKW5_002196"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO7511590.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            15149..17536
                     /gene="pheT"
                     /locus_tag="ACHKW5_002197"
     CDS             15149..17536
                     /gene="pheT"
                     /locus_tag="ACHKW5_002197"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO7511591.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHSLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGKRIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17541..17840
                     /gene="ihfA"
                     /locus_tag="ACHKW5_002198"
     CDS             17541..17840
                     /gene="ihfA"
                     /locus_tag="ACHKW5_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO7511592.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17941..18921
                     /gene="btuC"
                     /locus_tag="ACHKW5_002199"
     CDS             17941..18921
                     /gene="btuC"
                     /locus_tag="ACHKW5_002199"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO7511593.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            18984..19535
                     /gene="btuE"
                     /locus_tag="ACHKW5_002200"
     CDS             18984..19535
                     /gene="btuE"
                     /locus_tag="ACHKW5_002200"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO7511594.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19535..20284
                     /gene="btuD"
                     /locus_tag="ACHKW5_002201"
     CDS             19535..20284
                     /gene="btuD"
                     /locus_tag="ACHKW5_002201"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO7511595.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            20362..20826
                     /gene="nlpC"
                     /locus_tag="ACHKW5_002202"
     CDS             20362..20826
                     /gene="nlpC"
                     /locus_tag="ACHKW5_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO7511596.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            21073..21786
                     /gene="ydiV"
                     /locus_tag="ACHKW5_002203"
     CDS             21073..21786
                     /gene="ydiV"
                     /locus_tag="ACHKW5_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO7511597.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21849..23285
                     /gene="selO"
                     /locus_tag="ACHKW5_002204"
     CDS             21849..23285
                     /gene="selO"
                     /locus_tag="ACHKW5_002204"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO7511598.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(23289..23480)
                     /gene="hemP"
                     /locus_tag="ACHKW5_002205"
     CDS             complement(23289..23480)
                     /gene="hemP"
                     /locus_tag="ACHKW5_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO7511599.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(23612..24658)
                     /gene="aroH"
                     /locus_tag="ACHKW5_002206"
     CDS             complement(23612..24658)
                     /gene="aroH"
                     /locus_tag="ACHKW5_002206"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO7511600.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24815..25648)
                     /gene="ppsR"
                     /locus_tag="ACHKW5_002207"
     CDS             complement(24815..25648)
                     /gene="ppsR"
                     /locus_tag="ACHKW5_002207"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO7511601.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            25981..28359
                     /gene="ppsA"
                     /locus_tag="ACHKW5_002208"
     CDS             25981..28359
                     /gene="ppsA"
                     /locus_tag="ACHKW5_002208"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO7511602.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(28416..30062)
                     /gene="fadK"
                     /locus_tag="ACHKW5_002209"
     CDS             complement(28416..30062)
                     /gene="fadK"
                     /locus_tag="ACHKW5_002209"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO7511603.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCVRLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(30119..30412)
                     /gene="ydiT"
                     /locus_tag="ACHKW5_002210"
     CDS             complement(30119..30412)
                     /gene="ydiT"
                     /locus_tag="ACHKW5_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7511604.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(30409..31698)
                     /gene="ydiS"
                     /locus_tag="ACHKW5_002211"
     CDS             complement(30409..31698)
                     /gene="ydiS"
                     /locus_tag="ACHKW5_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7511605.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(31754..32692)
                     /gene="ydiR"
                     /locus_tag="ACHKW5_002212"
     CDS             complement(31754..32692)
                     /gene="ydiR"
                     /locus_tag="ACHKW5_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO7511606.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(32712..33476)
                     /gene="ydiQ"
                     /locus_tag="ACHKW5_002213"
     CDS             complement(32712..33476)
                     /gene="ydiQ"
                     /locus_tag="ACHKW5_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO7511607.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASGIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            33792..34238
                     /locus_tag="ACHKW5_002214"
                     /pseudo
     CDS             33792..34238
                     /locus_tag="ACHKW5_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT         8686 a         8167 c         8730 g         8709 t
ORIGIN      
        1 atattaaaag cctggctttc gactctttta aagaaaggct tgagtctacc atggcggcac
       61 tcactttctt tatatcaacg ggtcaatgtg actgcgatga aatagcagag tcgaacttca
      121 atcgtatgat tgcctatatg tctaacatta actatgatgc ctcaaagcca ggagcgccag
      181 cacttagttt tgatacgtat ctgcaggaca atgttaaata tagagtgata attaataatc
      241 tttatggcag tgagtttcgg ataagagata ttaatgagaa ttttgttggg atggatttta
      301 cctgtgaatt ctgacccgaa aaaatgacga atttgatcaa tataaaaata ggttggttat
      361 tcattattta aggactatat actatgaaaa aaattatata catcctgccg ggagcatttt
      421 tgctgcgatt caaaaaaaaa gagagtctcc taaagtttcc ttcgataata tcaatgctta
      481 atatcaatct tctttttaat ccactaaatc ttccgggaat gggaagtggt gtgcttgaag
      541 atattatgag tattccagat agttcactta gaaaacgtct cgggtatgag gttttaagct
      601 tctcactaca ggcacattca ttgagccagg aatgtattga taagttggac atttttttcg
      661 ctgacgatct gtttaaatat gagtcagtat gcatcgccgc gatggaacat ttaaaaagta
      721 aagcaacggc ccctatacaa aacggaccgt tgccagcata agaattaacg gtgtgccagt
      781 tcggcgtcgt cttcgctatc cagaatggct ttatcggttt gcttcagcca ctggctggtc
      841 agcgtgccag ctgtcatcga gccactaacg tttagcgccg tacggcccat gtcgataagc
      901 ggttcaacgg agattaacag cgccaccagg gttactggca ggcccatcgc aggcagcaca
      961 atcagtgcgg cgaaagttgc accaccaccg acaccggcaa cgcctgcgga actaacggta
     1021 acaataccga ccagcgtcgc aatccacatc gggtccagcg ggttaatgcc aaccgtaggc
     1081 gcaaccatca ccgccagcat tgccggatac aaaccggcgc agccgttctg accaatggtt
     1141 gcaccgaaag aggcggcgaa actggcgatg gattcaggaa cgcccagacg acgcgtttgt
     1201 gcttccacat tcagtgggat agacgcagca ctggaacggc tggtaaaggc aaacgtcagc
     1261 acaggccata ccttacggaa gtacttcagc ggactcacgc cattaatgcc cagcagaatg
     1321 ccatgcactg caaacataat cagcagaccg aggtaggacg cgacaacgaa acttcccagt
     1381 ttgatgatgt cttgcaagtt agaacctgca accactttgg tcattagagc cagaacgccg
     1441 taaggggtca attgcatgac aaggcgaacc agtttcatca cccagctttg tagggtatcg
     1501 atagcggcta agacgcgttc acctttcggc gcatcatcct tcagcagttt caatgcagct
     1561 acgccgagga atgcggcaaa aattaccacg ctgataattg acgtcggatt ggctccggta
     1621 agatcggcaa acgggttttt cgggataaag gacaagacca gctgcggaac gctcagatca
     1681 gagactttac caacatagtt actttcaatg gcgttcagac gtgcagtttc tgcaccaccc
     1741 tgaaccagac cttcagccgt caaaccaaac aggttggtga ccagcacacc gaccagcgcc
     1801 gcaatcagcg tggtaaacaa aagcgtaccg atggtcagaa aactgatttt gcctaactga
     1861 gatgcgttat gcagacgggc aaccgcgctc agaatagagg cgaagactaa cggcataacg
     1921 atcatttgca gcagttgaac atagccgtta ccaacgatgt taaaccactg tacagaatct
     1981 ttaagtacct ggctgtcaga accataaatg gtatgcaggg caaggccaaa aaccacaccc
     2041 atcaccagac ccaccagcac ttttttcgcc agactccact gtttatggcg ggtctgagcc
     2101 agcgcaaaca gcagtacaac gaacaccacg atgttcgcaa ttaatggaaa gttcatcccc
     2161 gttctcctga tcttattatt tctatcactg cgtagcagtc atagtgttgg cgcaagatta
     2221 acagaactgt gagctggctc ttatattcaa atggaattgc ttattccaga aaggtgattt
     2281 ttgtcttttt agtattcaac ctgatgctta ataagccgat gaaactggat ctgcaaggta
     2341 ttgatcattt gcgatgacca acgaacagcg ctgttctcgg gtaatgaatg gggcatccat
     2401 accgaccaga caaataatgc catgcagata aaacgttcca gttggttgcc cttttgggga
     2461 accaggatag gcaagcggaa acgccagcgg catggccaga gcaggggaac accggcgggt
     2521 gtcagcatat cggcaagtat gtggctcaaa taacccagca ccattccttg tagcgcatca
     2581 gccggaatga accagccttc cggaacctta aggtaaaagg ttgccagcag cgcaaatacc
     2641 gccagcagac tgtgggtaaa accacgatgc ccaaaagcgc gggcgatcgg ttttgatatc
     2701 cattttaatc gctgcccaag aaacgacttt gggtgatcga tgtccggtaa caaacacgtc
     2761 aggattgcgg aagggacaat atgccaccag tcaccctgtg ccagcacggg cgtcagctcg
     2821 gcatttttgg caaataccgc acaagcaata gaaaagagaa ggtgaccttc cgccgtcatg
     2881 ataaaactca ctaaactgtc gatacgtaca gtataggatt tttatacagt gcacggaagt
     2941 ggtgacggtg taactctttc tcttaaattg acgattaatc cgtcagttga atgcaaattc
     3001 agataaagaa ccaggggcag cgagatcact gcccctggtc attaaccgag aaggtctttt
     3061 gcggtgagtt ctgtcagcga tgagagtttg acgtttgcta atacaaaacg tggatcattt
     3121 tgcgcttctg gcgcaggaac gacaatggaa cgcatgcggg ctgctttaga ggcgatcatg
     3181 ccatttactg aatcttccag cgctacgcag gtcagtgggt caacgcccag ttttgctgcg
     3241 cagtcgagat atacttgcgg atgcggcttg ctgtaaggca gtttttcagc cgaggcgagg
     3301 gcatcgaaac tgtcgcgtaa gtcaaacatg gtcaacactt tttccagcat atgtagtggt
     3361 gacgcggagg ccagtcccac caataaacct tgttctttgc ataacgcaac ggcttcgcgc
     3421 acgcctggta ataatggacg tgtctcttca accagtgaaa tggcacgggc aataacccgt
     3481 tctactactt cctgacggct tggcccattc cacggttgcc gggcgtacca aagatcgacc
     3541 accatatcga tgcgtaaacc taaggtgtcc ggcagctcgt tacgacggga gatatccacc
     3601 cccaggcttg ccatcacatc cagttcggct cgatcccata aaggttctga gtcgataagt
     3661 aatccatcca tatcaaaaat tgcagcaaga atctgacgcg gggttgacat tacaacctct
     3721 cctttagagc attatgtgta aggtattaag ccgtgcttat gattaagcgc ctatcatatc
     3781 gcctttaaag aatgggcgaa aacagatatc tacaggtaaa ctaagtgaaa cgacgcgtct
     3841 taatcaagga gaactcatga cgtatcaaca agctggacgc attgctgttt tgaaaaggat
     3901 tttgggttgg gtgattttta tacctgcatt gatctctaca ttgatttctt tgctgaaatt
     3961 tatgaatacc cggcaggaaa accaggaagg cattaatgcg gtcatgctcg actttactca
     4021 tgtcatgatc gatatgatgc aggcgaatac gccttttttg aatctgttct ggtataactc
     4081 cccgacccct aattttaatg gtggcgtgaa cgttatgttc tgggtgattt tcatcctgat
     4141 ttttgtcgga ctggcgttgc aggattccgg tgcccggatg agtcgccagg cgcgcttctt
     4201 gcgggaaggt gttgaagatc aactcattct ggaaaaagcc aaaggggaag aggggctaac
     4261 gcgtgaacaa attgagtccc gtattgttgt tccgcaccat actattttcc tgcagttttt
     4321 ctcactatat atcctgccgg ttatttgtat tgctgcaggt tatgtgttct tttctctgct
     4381 tgggtttatt taatcaaaag gccgcacgga gcggccatta tcctcaaccc atatcatgct
     4441 gctaataatc tatccaatga ctgctgagca ataaccaaat gctgaccgcc aaataatctt
     4501 gcacgattta acagcgtgta gagttggtaa accggttgac gttcaaggaa atcggcaggt
     4561 agcggggata ctgactgata gccgtcatag atttgtggcg gctgttcagt atgcagcggt
     4621 aacatcgcca ggtcgcactc tcggtcaccc cagtagcagg cagggtcgaa aatgtacggg
     4681 ccatccggac ccagcgcaca gttgccggac cataaatcgc cgtgcaacag agaaggctgc
     4741 ggttgatgcg aggccagacg ctgctgaata tgctcgacga tggcgtcgat attgccgaaa
     4801 gcgatccctt tctctgctgc cagttccaac tgccagccaa tccgttgttc agcaaaaaac
     4861 gttgaccagc gacgttgcca ggtgttgggc tgtggagttg tggagagcga gttatcaaaa
     4921 tcgaggccaa attgtggttg gtcactccat tgatgtaaac gcgcaatttg ctgaccaaga
     4981 ataaatgcgc tatgcgcatc cagcggacga ggtgggagat aatccatcac cagaaaactg
     5041 tagtcacggt cagcgcctac tgcccaaacc ttaggcacgg tgacggtttt actacgcgac
     5101 agtaactcca gttggtcggc ttcggcggta aaaccgggaa gcagttccct ttcatcacat
     5161 ttgacgaaaa agtcatgtcc tgcatagcgc aaatgccatg cggcatggac ttctccgcca
     5221 ggcagttcat tacgcagttc gatttcgcct tcacctaact gctcgctcaa aagacgactg
     5281 attgcctgcc acatgatgtc tctcccatgt tgtctgccag atcataaggt tagcgcaaga
     5341 atgcggtgta aaaacacgag ctaacgcaca ccacagactg attattgccc aaaaaccatt
     5401 tattttctgt tgctattcca ttcctcccag gtcgtgatgg tgatttcagg atctttgccg
     5461 gttgcacttt gggtaagccc cgaaaccagt tctctaattt cttcttcact ttgggtactg
     5521 ataagtccaa aagtgttagt ccccagctca tggatatttc cctcatcatc cgtcatggtg
     5581 agcaaaaaac cgtcgcgagt caggtaatta ttcaactcgt taatttcagt caatgtatcc
     5641 tcatgcaaca tgacggttat gacataacgg acaagatcgc cactggccat agttcacccc
     5701 tttgttatca catgactttt gagcatagtc ggagaaacgc gttgccgaca ggttggtggt
     5761 gattccccca atgctggggg aatgtttttg ttagcgggaa aggtaagcgt aaattttttg
     5821 cgtatcgtca tgggagcaca gacgtgttcc ctgattgagt gtggctgcac tccccgcagc
     5881 tacgccaaaa cggaccatct cttcaagaga ggcattttct gccagtttca gtgtcatcgc
     5941 gccgaccatg ctgtcaccag cgccaacggt actctggctt ttcaccggtg gtggcaccac
     6001 ctgaatgcag ttttcactat caacacccag cgctccttgt ggaccaaggg aaacgacaac
     6061 ccgtttggcc ttgccgctat taacgatttc ctgcgcggct ttgcggacat cgtctggctg
     6121 ggtgagttcg cgattcacca gcgcactgag ttctttttgg ttaggcttaa ccaactcgat
     6181 gttaccaatc gccagtgctg cacttaacgc ttcgccagaa ctgtcgatga tgcagcggat
     6241 cccttggttt tgcgcagcgg aaatcagttg ggttaatttt tccagcttca cacctggcgg
     6301 caggcttccg cttatgacca ggatggcccc ggattcaatt tccagaactt gctcttcaag
     6361 ctggcgaaac tcatcttcat ttaatgccgc gcctggcata acaaaacgat actgctcacc
     6421 gcttgcttcc acatgtacgt gtaaattctg ccgggtccag tctttggctt ctacagtagc
     6481 gacggggaca ttttcatccg ccaacagaga aaccaggtgt tcgccggtcg cgccacccgc
     6541 cgggaagatc gctgtggcac tgcctccaag atgggcaatg gcgcgggcga cgttgatgcc
     6601 gccgcccccg ggttcgaaca ccggtgcggt acagcgcagt tttccttcgg gataaatttg
     6661 cggggtaatt gttgcgctat cgagagaggg cgcaagtgtc aacgtataga tacgtaccat
     6721 catttcctcc tataggctga tttcagtctg gcaccgaatc agccgaaagt gaagaaatta
     6781 acaatatgat ttattagagc tttatttaaa attttgcaga taaatatata taaataaaaa
     6841 tctgttttcg ccacatgctt attcaaaaaa tgaaataaga atacattgct aagttattcg
     6901 cgccttttta taattcgtta cctttccagg gacgctaatc tttgatcctt aagaaagttt
     6961 ccgggaccgc gatggtctct ttttttgttg tacggactct tgataaatga agcttttgaa
     7021 gacagttcct gccatcgtta tgttggcggg gggcatgttt gcgtcactga atgcagctgc
     7081 cgatgattcc gtttttactg tcatggacga tcctgccagc gctaaaaaac ctttcgaagg
     7141 taatctgaac gcaggatatc ttgcacaatc aggcaatacg aaaagctcct cacttacggc
     7201 tgacaccacc atgacctggt atggacaaac cactgcctgg tcgctgtggg ggaacgccag
     7261 taatacttct tctaatgatg agcgttcttc agaaaaatat gcggcgggcg ggcgtagccg
     7321 ttttaattta actgattatg actatttatt tggtcaggca agctggctga cagaccgtta
     7381 taacggctat cgagagcgcg atgtgttgac cgcgggttat ggtcgtcagt ttttgaatgg
     7441 cccggtacac agtttccgct ttgaattcgg tccaggtgtg cgttacgaca aatataccga
     7501 caatgccagt gaaactcagc cgctgggcta tgcctccggt gcttatgcat ggcagttgac
     7561 tgacaacgct aaatttacgc agggtgtttc cgtctttggc gcggaagaca caacgctgaa
     7621 ctccgaaagc gcattaaatg ttgctattaa tgaacatttt ggactgaaag tggcttacaa
     7681 cgtcacatgg aactctgaac ctccagagtc tgcaccggaa cataccgatc gtcggacaac
     7741 cttatcgctg ggctacagca tgtaaaatta tctgccggac cgtgttatgc ggtctggctg
     7801 tcttcgtttg tcactgctca gccccaaatc attgctgccc aacgtagcaa caacatagcg
     7861 ccacagattg ccagtagcac caccaccatc ttccagtgtt ttttatcaaa gcgtttagtc
     7921 ggcataccag tgtcctgcat atctcttgtg cgtgataacg gagtgtaaca aaagggacag
     7981 gcatcaaggg attttttgtt attacagccg tcgggtggcg ttgtacacct gatcactgtt
     8041 tctgcgtttt atgaaaaaaa taaaattatg tatcgatata caaatatcat aaatagctgg
     8101 ttataaatac gtttttagtt catcagcact aaaactcagg ctttggctga ttgcattaat
     8161 agcagcataa tatgtttcta cagttttata atgattatga tgaatggcta tatgaacagc
     8221 tgacatgttt tcatgatttt tagcggccaa cagtgtgaat aattgacgtt gactaaaaga
     8281 atattttttt gcaaaagtac tcagtgttga tatataagat aacacgacgt ctttattacc
     8341 cttgctcatg gcgatgtata aagcaggggt tccatgtgct gtcgcgcctt ttaataagtc
     8401 catgatgttg attttgctga gtttatattt aacggcgatc ccataaactt tagagaggaa
     8461 ccgtgtggca cacaaagggt gattattttc cattgcagca aataaccctg gttcaccatc
     8521 acgtgttttc atggctaaaa tatccatcac catttttttg tttaaaagtt cttgtgctat
     8581 tggagtggtt aatgcattca gaaagatatc gattatttca tcgaatccat acaacatgga
     8641 gatatagagt ccaggtgttt cgttagcact tttagtttgc agtaatttaa ccagattatc
     8701 ttcatgaatg atatgattgt ataccagtga ctggatttct tgcataaata tttttacgtt
     8761 gtcagtttgt ccgttcatca tagctagaaa taaagcaggt gtaccaacgc tattctgcgc
     8821 agttattatt tcaatgattt ctttttcagg gagtatgggc aactgtttga atagtgtaat
     8881 aagctattga gtctggcctg tggccattat taacaacatt gccgatggat gtataggggc
     8941 aaatggcatt cgtgtcgtga tacccgctaa aatagcaact ggaactccct caggtaatag
     9001 aggaagaata gcaattgcac agttgcgaac atacctatca gcccaggtct gagcatcggg
     9061 agctgaactg atgaacttat caagcgagag aaagcgccgt gctgtcctgt gggtgcctct
     9121 gacggcaacg ttggtatcgt tcgggtcgta aacagacaca acataattta attgtccata
     9181 cactgactgc tttattcgta gccttactga caaagtatga ttgtctatat ataaaatcgc
     9241 ggacatatga ctgacattgt ttgccatcat tcgacaaaag aaaatatcta aagcgaagcc
     9301 aaaatcctta ctcgcgataa cgcgactccc tgcaggagga tggcgaagta atgctctgaa
     9361 ctccgcatcc cggactaagg ggatatgctg ctgtatggat tcaacggagg agtaatgttt
     9421 aatcaggttg actttgcctt ttccatttct caatgaatca agggcgtatt gcaatgacag
     9481 atggcgacaa aaaatagcgt cagaaggaga ttgcaaaaaa catgcactac cattgagatt
     9541 tactgttggg tgtatagttt tttctctatg gattttttta gacagtttca tacaatggtt
     9601 acgatcttca gcagttaact gttcctgttc aatataatcc agtatgcttc gcaaatggtt
     9661 agctttaata ggcgtggttt ccagtaataa ggaaatgaaa tcttttttat agtgtaatgt
     9721 aatgagtata ttttctgcat ggatgggtga agtggcaaag ttaatggtaa aaagggcagt
     9781 ggaataacaa ttttgtgata tttttcgaca gtttgcggta aattcctgaa taacacagta
     9841 tgacagcatc gaacttaagt cagaggtatc aagtagggtg atgagctggc gcgcaaattt
     9901 attaataggc tctgatgaag gtgttggtat gtcgctatcg atgtgcaatc tatgaggaga
     9961 cttataatga gttctgataa tgatatcgtt ttgactcata atctattcgc cttatatata
    10021 tctggaggtt ttgaggatga tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa
    10081 ttgttataag aattgtgtca cttttatcat tgtgatatga ccacttcgtc attaatctca
    10141 tgagcgttta aaatactacc gccaaatcac gcaacgaact ttgctggatt cagtttatgc
    10201 tgctgtaaat ccgctcgcag taaacctttc agacgcaccg tgatgttatc agtggttctt
    10261 taagcgtttt gctggtgtac tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg
    10321 aaaggtacaa tctccctttt tttagtgtta acatcgctca accgggtgga aggtgagagg
    10381 ttaatgtctt tcatttgtgt acctgattgc gaaccaattt agcatttgtt ggctagatgg
    10441 tttcgcaatg aactgttaat aaacaaattt ttctttgtat gtgatctttc gtgtgggtca
    10501 ccactgcaaa taaggatata aaatgcctgt tataactctt cctgatggca gccaacgcca
    10561 ttacgaccac gctgtaagcc ccatggatgt tgcgctggac attggtccag gtctggcgaa
    10621 agcctgtatc gcagggcgcg ttaatggcga actggttgat gcttgcgatc tgattgaaaa
    10681 cgacgcacaa ctgtccatca ttaccgccaa agacgaagac ggtctggaga tcattcgtca
    10741 ctcctgtgcg cacctgttag ggcacgcgat taaacaactt tggccgcata ccaaaatggc
    10801 aatcggcccg gttattgaca acggttttta ttacgacgtt gatcttgacc gcacgttaac
    10861 ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag cttgctgaga aaaactacga
    10921 cgtcattaag aagaaagtca gctggcacga agcgcgtgaa actttcgcca accgtgggga
    10981 gagctacaaa gtctccattc ttgacgaaaa catcgcccat gatgacaagc caggtctgta
    11041 cttccatgaa gaatatgtcg atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg
    11101 ccatcatttc aaactaatga aaacggcagg ggcttactgg cgtggcgaca gcaacaacaa
    11161 aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa aaagcgctta acgcttacct
    11221 gcagcgcctg gaagaagccg cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct
    11281 gtaccatatg caggaagaag cgccgggtat ggtattctgg cacaacgacg gctggaccat
    11341 cttccgtgaa ctagaagtgt ttgttcgttc taaactgaaa gagtaccagt atcaggaagt
    11401 taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa accggtcact gggacaacta
    11461 caaagatgca atgttcacca cgtcttctga gaaccgtgaa tactgcatta agccgatgaa
    11521 ctgcccgggt cacgtacaaa ttttcaacca ggggctgaag tcttatcgcg atctgccgct
    11581 gcgtatggcc gagtttggta gctgccaccg taatgagccg tcaggttcgc tgcatggcct
    11641 gatgcgcgtg cgtggattta cccaggatga cgcgcatatc ttctgtactg aagaacaaat
    11701 tcgcgatgaa gttaacggat gtatccgttt agtctatgat atgtacagca cttttggctt
    11761 cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa cgtattggca gtgatgaaat
    11821 gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa gaaaacaaca tcccgtttga
    11881 atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt gaatttaccc tgtatgactg
    11941 cctcgatcgt gcatggcagt gcggtacagt acagctggac ttctctttgc cttctcgtct
    12001 gagcgcttct tatgtaggcg aagacaatga acgtaaagta ccggtaatga ttcaccgcgc
    12061 aattctggga tcgatggaac gtttcatcgg tatcctgacc gaagaattcg ctggtttctt
    12121 cccgacctgg cttgcgccag ttcaggttgt tatcatgaat attaccgatt cacagtctga
    12181 atacgttaac gaattgacgc aaaaactatc aaatgcgggc attcgtgtta aagcagactt
    12241 gagaaatgag aagattggct ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat
    12301 gctggtctgt ggtgataaag aggtggaatc aggcaaagtt gccgttcgca cccgccgtgg
    12361 taaagacctg ggaagcatgg acgtaaatga agtgatcgag aagctgcaac aagagattcg
    12421 cagccgcagt cttaaacaat tggaggaata aggtattaaa ggcggaaaac gagttcaaac
    12481 ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag gaagttcgct taacaggtct
    12541 ggaaggcgag cagcttggta ttgtgagtct gagagaagct ctggagaaag cagaagaagc
    12601 cggagtagac ttagtcgaga tcagccctaa cgccgagccg ccggtttgtc gtataatgga
    12661 ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa
    12721 agttatccag gttaaggaaa ttaaattccg tcctggtaca gatgaaggcg actatcaggt
    12781 aaaactccgc agcctgattc gctttctcga agagggtgat aaagccaaaa tcacgctgcg
    12841 tttccgcggt cgtgagatgg cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa
    12901 agacgatttg caagaactgg cagtggtcga atccttccca acgaagatcg aaggccgcca
    12961 gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc cttcaagtag cactgtctgt
    13021 ggagccttcg ggcttcacag gctttgttcg cctttgtttc gtttattaac aatgcgaagt
    13081 ggaagttatt aaaatgccaa aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa
    13141 aaccggtaaa ggtggtttta agcacaagca cgctaacctg cgtcacattc tgaccaaaaa
    13201 agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg gtttccaaag gcgatctggg
    13261 cctggtaatc gcgtgcctgc cgtacgcata agccgttaac gtttttaact ttttaattag
    13321 aatatagata caggagagca catatggctc gcgtaaaacg tggtgttatt gcacgcgcac
    13381 gtcacaagaa aattttgaaa caagctaaag gctactacgg tgcgcgttct cgcgtatacc
    13441 gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac
    13501 gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg
    13561 gtatttctta cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta
    13621 agatcctggc tgatatcgca gtattcgaca aagtagcgtt caccgctctg gttgaaaaag
    13681 cgaaagcagc tctggcataa gccagttgaa agagggagct agtctccctc ttttcgtttc
    13741 aacgccatca aaacattgac ttttatcgcc gtagcctttt caataaaggt cttttgaaga
    13801 gtaccaaaag gtaacgcaag caatgaatgc tgctattttc cgcttctttt tttactttag
    13861 cacctgaatc caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct
    13921 cccagtggag gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggt
    13981 gtaattgtcg cagccagtct ggacacggcc agcgcggaga aaccggagcg tatttaagta
    14041 cgtgagaatt tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggata
    14101 ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaacc atgtcacatc
    14161 tcgcagaatt ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt
    14221 tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga
    14281 ccctgcgtga gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga
    14341 aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gctgcactga
    14401 atgcgcgtct ggcggcggaa acgattgatg tctctctgcc aggtcgtcgc attgaaaacg
    14461 gcggtctgca tccggttacc cgtactatcg accgtatcga aagtttcttc ggtgagcttg
    14521 gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga
    14581 acattcctgg tcaccacccg gcgcgcgctg accacgacac tttctggttt gacactaccc
    14641 gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagcc cagcagccac
    14701 cgattcgtat cattgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc
    14761 cgatgttcca tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga
    14821 aaggcactct gcacgatttc ctgcgtaact tctttgagga agatttgcag attcgcttcc
    14881 gtccttccta cttcccgttc accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg
    14941 gtaaatggct ggaagtgctg ggttgcggga tggtgcatcc gaacgtgctg cgtaacgttg
    15001 gcatcgaccc ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacta
    15061 tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga aaatgatctg cgtttcctca
    15121 aacagtttaa ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg
    15181 ggtgaacccg gcgattgata gcgatgcgct ggcgaatcaa atcactatgg cgggcctgga
    15241 agttgacggt gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtggt
    15301 tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgttggcgg
    15361 cgatcgcctg ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc
    15421 ggtagctacc attggtgctg ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg
    15481 tggtgaaccg tctgaaggga tgctgtgctc cttttctgag ctgggaattt ctgacgatca
    15541 tagcggcatt atcgagctgc ctgcggatgc gccgattggc accgacatcc gtgaatacct
    15601 gaaacttgat gacaacacca tcgaaatcag cgtgacgcca aaccgtgccg actgtttagg
    15661 tatcattggt gttgcgcgtg acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga
    15721 aatcgttccg gttggtgcga ccatcgacga cacgctgccg attacagtcg aagcgccgga
    15781 agcctgcccg cgttatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc
    15841 gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga
    15901 cgtcaccaac tatgtactgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg
    15961 cattgaaggc ggcattgtgg tgcggatggc gaaagagggc gaaacgttgg tactgcttga
    16021 tggcactgaa gcgaagctga atgctgacac tctggtcatc gccgaccaca acaaggcgct
    16081 ggcgatgggc ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt
    16141 cttgctggaa tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatag
    16201 cctgcatact gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa
    16261 agcgatggaa cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt
    16321 aattgatatc accaacgaag caacgctgcc gaagcgtgca accatcactt tacgtcgtag
    16381 caaactggat cgcctgatcg gccatcatat tgcggatgag caggtaactg acattctgcg
    16441 tcgtctcggc tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg cgccgagctg
    16501 gcgtttcgat atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta
    16561 caacaacatc ccggatgagc cggtacaggc aagcctgatt atgggtactc accgtgaagc
    16621 tgacctgtcg ctcaagcgcg tgaaaacgct gctcaacgac aaaggctatc aggaagtgat
    16681 cacctacagc ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt
    16741 actgctgcca agcccgatct ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg
    16801 cctgctggca accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga
    16861 aagcggtctg cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat
    16921 gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac
    16981 cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact
    17041 gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc
    17101 gatttatctg aaaggtaaac gtattggttt tgttggggtt gttcatcctg aactggaacg
    17161 taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga
    17221 ccgcgtggtg cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc
    17281 ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg
    17341 cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga
    17401 ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga
    17461 agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc
    17521 atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg
    17581 ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga
    17641 tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc
    17701 tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta
    17761 cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg
    17821 cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct
    17881 tttcactgtc gaagagtcac cgtaaaatca acgccatgac acttcagcag aacggatacc
    17941 atgctgacac ttgcccgcca acaacagcga caaaatattc gctggttatt atgcctgtca
    18001 gttttgatgc tgctggcgct tctcttaagc ctttgcgccg gtgaacaatg gatctcgcca
    18061 ggtgactggt ttactcctcg tggcgaactg ttcgtctggc aaattcgcct gccacgtacg
    18121 ctggctgtat tgctggttgg tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg
    18181 tttgaaaatc ctctggcaga acctggacta cttggcgtct ctaacggcgc aggcgtgggg
    18241 cttatcgccg cggtattgct tgggcaaggg caactcccca actgggcgct agggctgtgt
    18301 gcgattgctg gcgcgcttat catcacttta atactcttac gtttcgcccg tcgtcatctt
    18361 tcgaccagtc ggttattgct ggctggcgtt gcattaggga ttatctgtag cgcactaatg
    18421 acgtgggcta tctacttttc cacctctgtt gatttacgtc agctgatgta ctggatgatg
    18481 ggcggttttg gcggcgtaga ctggcggcaa agctggctga tgctggcatt gatccccgtg
    18541 ttgttgtgga tctgttgtca gtccaggccg atgaatatgt tagcacttgg cgagatctcg
    18601 gcgcggcaac tgggtttacc cctgtggttc tggcgcaatg tgctggtggc cgcgaccggc
    18661 tggatggttg gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg tctggtgatc
    18721 ccacatattc ttcggttgtg tggtttaacc gatcatcgcg tattacttcc cggctgcgcg
    18781 ctggcagggg cgagcgcatt gctgctggcc gatattgtag cgcgcctggc attagctgcc
    18841 gcagagctgc ctattggcgt ggtcaccgca acgttgggtg cgccggtgtt tatctggtta
    18901 ttgttaaaag caagacgtta gccgcaaaaa gacggtctat gattaaaagc taagattttt
    18961 actgaccaca cccaggagaa acgatgcaag attccattct gacgaccgta gtgaaagata
    19021 tcgacggtga agtgaccacg ctggagaagt acgctggtaa tgtgctgttg attgtcaatg
    19081 tcgcctcaaa gtgtggctta acgccgcaat atgagcagtt ggagaatatt cagaaagcct
    19141 gggccgatcg aggttttgtg gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac
    19201 cgggcagcga tgaagagatt aaaacttact gtaccaccac atggggggtg acgttcccga
    19261 tgttcagtaa gattgaagtt aatggcgaag gacgccatcc gctgtatcaa aaattgattg
    19321 ccgcagcgcc gaccgcagtc gcgccggaag agagcggatt ctatgcccgt atggtcagca
    19381 aaggtcgtgc accgctgtac cctgatgata ttttatggaa ttttgagaaa tttttagttg
    19441 gcagggacgg gttggtcatc cagcgttttt ccccggatat gacgcctgaa gatcccattg
    19501 tgatggaaag cattaaactg gcgttggcaa aataatgtct attgtgatgc agttacagga
    19561 tgttgcggaa actacccgcc tggggccgct ttctggcgag gttcgggctg gggagatcct
    19621 gcacctggtg gggccgaatg gcgcgggtaa gagtaccttg cttgcacgaa tggccggaat
    19681 gaccagcggt aagggaagca ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac
    19741 aaaactcgcg ctgcatcgcg cctacctttc acaacagcag acgccgccgt ttgcaatgcc
    19801 ggtctggcac tacctgacac tgcatcagca cgataaaacg cgtaccgaac tactgaatga
    19861 tgtcgcaggg gcgctggctc ttgatgacaa actcggacgt agcaccaatc aactttccgg
    19921 cggtgaatgg caacgcgtac gtcttgctgc ggtggtgctg caaatcacac cacaagccaa
    19981 tcccgcaggt caattgctgc ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca
    20041 aagtgcgtta gacaaaattc tgagcgctct gtgtcagcaa ggactggcga ttgtgatgag
    20101 cagtcacgat ctcaaccaca cattgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg
    20161 aaaaatgctg gccagtggac gcagagaaga ggtgctcacg ccgccaaatc tggcgcaggc
    20221 ctatgggatg aattttcgcc gtctggatat cgaaggtcac agaatgctga tttcgaccat
    20281 ctgatttcat tagtatcttg aaaatcgggt aattacgacg ctaaattaat gcaagaataa
    20341 aaaacagagg aaccgtacgg aatgcgtttc tgccttattt tgatcacagc actgtttctg
    20401 gccgggtgta gccaccataa agcaccgccg ccaaatgcca gactttctga ttcgattacc
    20461 gttattgccg gtttgaacga ccagctacaa agctggcatg gcacgccgta tcgttatggt
    20521 ggcatgacgc ggcgcggtgt ggactgttcg ggatttgtgg ttgtaacgat gcgcgatcgt
    20581 ttcgatttgc agctgccccg agaaaccaaa gaacaagcct ctatcggcac gcaaattgat
    20641 aaagacgagt tgctgcctgg tgacctggtc tttttcaaaa cgggttccgg acaaaatggt
    20701 ttgcatgtag gtatttacga taccaacaac caatttatcc acgcctctac cagcaaggga
    20761 gtgatgcgtt cctcacttga taatgtctat tggcagaaaa atttctggca ggcgagacga
    20821 atttagtatg tcgaaaaatg gacggcgatg atacatcgcc gtccatttac tcctgaatac
    20881 agtagtattt tgcagacaaa aattccgtaa atggattatt cgtttataat caggataatc
    20941 taaaaaaagg acccctgaaa aagtggttaa catggaacaa atgaaagtta ctccttctgt
    21001 aattgttatg tcttatggcc tcatcccttt tagccggata ctgaaaaaca tccttcgaga
    21061 gggacggtta ccatgaagat ttttttggag aatctttatc attctgattg ctatttcctg
    21121 ccgataagag ataaccagca actccttgtc ggcgttgagt taattaccca tttctccagt
    21181 gaagatggca cggtgcgtat cccgacgagt cgggttatag cgcaacttac cgaggagcaa
    21241 cattggcaat tattttcaga gcagttggaa ttactaaaat catgccaaca tttttttatt
    21301 caacataaac tctttgcctg gcttaattta acgccacaag tggctacatt attattagag
    21361 agggataatt acgcaggcga actattaaaa tatccgttta ttgaactgtt gattaatgag
    21421 aattacccgc atctcaatga aggaaaagat aatcgcggtc ttttatcctt atcgcaagtg
    21481 tatccccttg tgttaggtaa tttgggagca ggcaatagca caatgaaagc ggtttttgat
    21541 ggtcttttca cccgcgtcat gctggataaa agttttattc agcaacaaat cacgcaccgc
    21601 tccttcgaac cgtttattcg tgcgatccag tcgcaaattt ccccttgttg caattgcatc
    21661 attgcgggag gcattgatac ggcagaaata ttggcacaga ttaccccctt cgatttccat
    21721 gctctccagg gatgcctgtg gcctgccgtt cccataaatc agataacgac gttggttcag
    21781 cgataaccct tctgtttgct ggtgtttaag acgagagtaa ccgtctacac tatcaaacag
    21841 gaggatctat gaccctgtct tttattaccc gctggcgcga tgaattgcca gaaacctata
    21901 cagcactttc ccctacgcct ttaaataatg cccggctgat ttggcataat accgaactcg
    21961 ctaacacgct gagtattcca tcgtcgctgt ttaaaaatgc tgcaggtgtc tggggcggcg
    22021 aaaccctact gcctggtatg tcaccactgg cgcaggttta cagtggtcat cagttcggcg
    22081 tctgggcggg ccaactgggt gatgggcgcg gcattttact tggcgaacaa ctgcttgctg
    22141 atggcactac aatggactgg catctgaaag gtgctggcct gacgccttat tcgcgaatgg
    22201 gtgatggacg ggcggtttta cgttcgacga tacgagaaag tctcgccagt gaggcgatgc
    22261 attatctggg cattccgacg acccgcgcgt taagtatcgt caccagcgat tcgccagtgt
    22321 atcgggaaac ggtggagcca ggcgcgatgc tgatacgtgt ggcaccaagt catctgcgct
    22381 ttggtcattt cgaacatttt tactatcgcc gcgagccgga aaaggttcgt cagttagctg
    22441 actttgccat tcgtcattac tggtcacatc ttgaagatga tgaggacaaa taccgtctct
    22501 ggtttaacga tgttgtcgca cgtaccgcat cgttaattgc ccaatggcag acggtcggct
    22561 ttgctcatgg ggtgatgaat accgacaaca tgtcgctgct ggggctgacg cttgattacg
    22621 ggccgtttgg ttttcttgat gattacgaac ccggttttat ttgtaatcac tcggatcatc
    22681 aagggcgtta cagctttgat aatcaacctg ccgtcgcgtt gtggaattta cagcgtctgg
    22741 cgcagacatt gtcaccattt gttgccgtag atgccctgaa tgaggccctg gacagctatc
    22801 agcaggtttt gttgacgcat tatggacaac ggatgcggca gaaactgggc ttcatgacgg
    22861 agcaaaaaga ggataacgcg ctactgaatg aattattcag tctgatggcg cgagagcgca
    22921 gcgattatac ccgcacattc cgcatgctga gtctgaccga gcagcacagc gcggcgtcac
    22981 cgctacgtga tgagtttatt gatcgtgcgg catttgatga ctggtttgcc cgttatcggg
    23041 ggcgtttgca acaagacgag gttagcgata gtgagcgtca gcagctgatg caaagcgtta
    23101 acccagctct ggtgttgcgc aactggttgg cgcaacgggc gattgaagcg gcagaaaagg
    23161 gtgatatgac ggaattgcac cgcctgcatg aggcgttgcg aaatcctttc agcgacagag
    23221 ctgatgacta tgtcagtcgt ccacctgact ggggtaaacg gctggaagtc agctgctcga
    23281 gttaaaggct acttggtcaa cagcagcttg ccagcctgag ttttacggag cagatattct
    23341 tgcccgtcat gatcgataat cagtttgcca tccggaccta acagtgtctg gctggaaatt
    23401 cgccgaatag gctgcgggga agcggtcttg tgattggcat ccgtttcagg cgtgagtttt
    23461 ctgctatccg tataacgcat aaaaattaat aataattgat ataacaatga ttcttattat
    23521 caatattatg gctgaccaga gcaagcagta atgtgctgag aagacaacaa gttgcgcaga
    23581 aatgcccatc ccatcaggaa tgggcacgca ttcagaagcg ggtatctacc gcagaggcaa
    23641 gtttttcgac caggcgttcg gtatcttccc agcccagaca cgggtcggta atggattgac
    23701 cgtaagtgag cggctgaccg ccgacgattt tttgcgttcc ttcgcgcagg aaactttccg
    23761 ccataattcc agcaatcgcc gtagagccat tgcggatttg ctgacaaata tcctcacaaa
    23821 cttctaactg gcgacggtgc tgcttctggc agttaccgtg gctgaaatcc accaccagat
    23881 gttcaggtaa atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca tcggcatgat
    23941 aattcggttt tttgccgcca cgcataataa tgtggccata cgggttgccg ctggtctgat
    24001 agatggtcat ctgaccattt ttgtctggcg agagaaacat atggctggcg cgggctgcgc
    24061 ggatagcatc cacggcaatc cgcgtgttgc catcggtacc atttttaaaa cctaccggac
    24121 aggagagtgc cgaagccatt tcacggtgga tctgactttc ggtagtacgt gcgccaatcg
    24181 cgccccaact gattaaatca gcaataaact gaccggtcac catatcgagg aactctgtcg
    24241 cggtagggac acccagctca tttacctgta aaagtaattt gcgcgccagc tccagaccgt
    24301 gatttacccg atagctgccg tttaaatccg gatcggagat tagccctttc cagccgacaa
    24361 cagttcgtgg tttttcaaaa taggtgcgca ttacgatttc cagccgtgac tggtactggt
    24421 tgcgcagcga ctgcagacgg gtggcgtact ccattgcagc ggtgagatcg tggatcgagc
    24481 aggggccaat aatgaccaac agtcgcttat cttcaccatt cagtattttt tcaattctgc
    24541 ggcgggagtc ggtgacatgg gtggcgacgc caggcgttac gggataccgt agcgcgagtt
    24601 cggcgggcgt taccaggctc tcaatacgcg cagtacggag ttcgtcagtt ctgttcatta
    24661 caagtctcag aaatttgttg cttctacggc aggactaccg ggaagtgatg cgcatcacga
    24721 taaaccaatc gcagctgatt tcaagtgcga ggtgtgtcac acctcgccgg gtggttagca
    24781 tgataacaaa aaaataagct aatgcactag ttctctagta cattcggcgg ctaagcccca
    24841 tgatatcgag gatcttggtg gcaatctctt ctaccgaata attggtactg ttaatccacg
    24901 ggatctgatt tttacggtac aacgcttcta cttccgcgac ttccatcctg cactgacgaa
    24961 gcgaggcata gcggctgttc tcccgacgtt cctcgcgaat cgccgccaga cgttccgggt
    25021 cgatagtcag gccgaacaat ttatgctgaa gcggtttgag cgacgcgggt agcaccagat
    25081 tgtccatatc gtcggcaata aaggggtagt ttgccgcgcg gataccaaac tgcattgcca
    25141 gatacagact ggtgggggtt ttaccacagc gagaaacacc gagcaggatc acctgagcct
    25201 ggtccagatt gcgcaacgaa atgccgtcat cgtgggcgag ggtgtaatca atcgccgcaa
    25261 tgcgcgcatc atatttattg agattattag ggttaaggcc atgggtacga tgagcaatcg
    25321 gcgttggatc cagtttcatc tcttgttgta gcggggcaac cagcgcctga acaatatcct
    25381 ggcaaaagcc ttcactttgc aagatgatgg cgcgaatctc cggcaacacg atggagtaga
    25441 agaccagcgg gcgcacacct gtctggtgat aaattgcgtc aatctgatcc ttcactgccc
    25501 gtgcacggct ctcattttcg acaaacggca gcgtaatgct gctgatagtg acgggaaatt
    25561 gtgacattac tgcgtgtcct aatacctccg cagttattgc cgtaccatca gaaatataaa
    25621 aaacgtggcg atcaacagca ttatccattt tgttcttccc gtgatgcaga cataattcta
    25681 tgaaagcata aattaaaaac gcacagaagc gtaggacgta atgtctggtt tataaaatga
    25741 accttcaatt ttatttttta tgaaaacagc atttcatttt tatggtttcg tttataccga
    25801 tggtttatgt ggaaattgtc gaatagagca ggtttgcgca acgctgggat cagtcttaaa
    25861 aagtaaaaaa atatatttgc ttgaacgatt caccgttttt ttcatccggt taaatatgca
    25921 aagataaatg cgcagaaatg tgtttctcaa accgttcatt tatcacaaaa ggattgttcg
    25981 atgtccaaca atggctcgtc accgctggtg ctttggtata accaactcgg catgaatgat
    26041 gtagacaggg ttgggggcaa aaatgcctcc ctgggtgaaa tgattactaa cctttccgga
    26101 atgggtgttt ccgttccgaa tggtttcgcc acaaccgccg acgcgtttaa ccagtttctg
    26161 gaccaaagcg gcgtaaacca gcgcatttat gaactgctgg ataaaacgga tattgacgat
    26221 gttacccagc ttgcgaaagc gggcgcgcaa atccgccagt ggattatcga cactcccttc
    26281 cagcctgagc tggaaaacgc catccgcgaa gcctatgcac agctttccgc cgatgacgaa
    26341 aacgcctctt ttgcggtgcg ctcctccgcc accgcagaag atatgccgga cgcttccttt
    26401 gccggtcagc aggaaacctt cctcaacgtt caggggtttg acgccgttct cgtggcagtg
    26461 aaacatgtat ttgcttctct gtttaacgat cgcgccatct cttatcgtgt gcaccagggt
    26521 tacgatcacc gtggcgtggc gctctccgcc ggtgttcaac ggatggtgcg ctctgacctc
    26581 gcatcatctg gcgttatgtt ctccattgat accgaatccg gctttgacca ggtggtgttt
    26641 atcacttccg catggggcct tggggaaatg gtcgtgcagg gtgcggttaa cccggatgag
    26701 ttttacgtcc ataaaccgac actggcggcg aatcgcccgg ctatcgtgcg ccgcaccatg
    26761 gggtcgaaaa aaatccgcat ggtttacgcg ccgacccagg agcacggcaa gcaggttaaa
    26821 atcgaagacg taccgcagga acagcgtgac atcttctcgc tgaccaacga agaagtgcag
    26881 gaactggcaa aacaggccgt acaaattgag aaacactacg gtcgcccaat ggatattgag
    26941 tgggcgaaag atggccacac cggtaaactg ttcattgtgc aggcgcgtcc ggaaaccgtg
    27001 cgctcacgcg gtcaggtcat ggagcgttat acgctgcatt cacagggtaa gattatcgcc
    27061 gaaggccgtg ctatcggtca tcgcatcggc gcgggtccgg tgaaagtcat ccatgacatc
    27121 agcgaaatga accgcatcga acctggcgac gtgctggtta ctgacatgac cgacccggac
    27181 tgggaaccga tcatgaagaa agcatctgcc atcgtcacca accgtggcgg tcgtacctgt
    27241 cacgcggcga tcatcgctcg tgaactgggc attccggcgg tagtgggctg tggagatgca
    27301 acagaacgga tgaaagacgg tgagaaagtc actgtttctt gtgccgaagg tgataccggt
    27361 tacgtctatg cggagttgct ggaatttagc gtgaaaagct ccagcgtaga aacgatgccg
    27421 gatctgccgt tgaaagtgat gatgaacgtc ggtaacccgg accgtgcttt cgacttcgcc
    27481 tgcctgccga acgaaggcgt gggccttgcg cgtctggaat ttatcatcaa ccgtatgatt
    27541 ggcgtccacc cacgcgcact gcttgagttt gacgatcagg aaccgcagtt gcaaaacgaa
    27601 atccgcgaga tgatgaaagg ttttgattct ccgcgtgaat tttacgttgg tcgtctgact
    27661 gaagggatcg cgacgctggg tgccgcgttt tatccgaagc gcgtcattgt ccgtctctct
    27721 gattttaaat cgaacgaata tgccaacctg gtcggtggtg agcgttacga gccagatgaa
    27781 gagaacccga tgctcggctt ccgtggcgcg ggccgctatg tttccgacag cttccgcgac
    27841 tgcttcgcgc tggagtgtga agcagtgaaa cgagtgcgca acgacatggg gctgaccaac
    27901 gttgagatca tgatcccgtt cgtgcgtacc gtagatcagg cgaaagcggt ggttgaagaa
    27961 ctggcgcgtc aggggctgaa acgtggcgag aacgggctga aaatcatcat gatgtgtgaa
    28021 attccgtcca acgccttgct ggcagagcag ttcctcgaat atttcgacgg cttctcaatt
    28081 ggctcaaacg atatgacaca gctggcgctc ggtctggatc gtgactccgg cgtggtgtct
    28141 gaattgttcg atgagcgcaa cgatgcggtg aaagcattgc tgtcgatggc tatccgtgcc
    28201 gcgaagaaac agggcaaata tgtcgggatt tgcggtcagg gtccgtccga ccatgaagac
    28261 tttgccgcat ggttgatgga agaggggatc gatagcctgt ctctgaaccc ggacaccgtg
    28321 gtgcaaacct ggttaagcct ggctgaactg aagaaataaa ataaatcccc ggcggcgttc
    28381 agtcgccggg tttatgtgat ccccgaagat gaaacttatt caatctcttc acagacatcc
    28441 tgcgttaatc gccgcataat atcttttctt aacaaaaact tttgtatttt acctgaggca
    28501 gtgcgcggta gtttttcgat taccacgata tgttcaggat atttatattt tgcgacccgt
    28561 ttacggctaa aaaaagccac cacctcttcc agcgataatg aatgatgcgg cgctttcagc
    28621 acgacataag cgcatgatcg ttcacctaaa cgttcatcgg gcattgcaac cacacaggca
    28681 tcgtgaattt taggatgctg caataaaata tcttccactt cacggctgct aatattttcg
    28741 ccgccgcgga caataatatc ttttttgcgt ccggtaattt ttatatagcc agcctcatcc
    28801 atacggcaga gatcgccgct gtaataccag ccttcttcat ccagcgcacg ggcggttaat
    28861 tcaggttcat caaaatatcc cataaacaca ttggggccac gcgaggcttc ttcaccttcg
    28921 caacctggcg gtaaggtctt gcgtgcgtca tcgaccactt taatctctac acctgcggca
    28981 gcgtaaccat cggtgtgcat aaagcgcgac aaaggatcat cgagattcac caccgcatgc
    29041 ggcgaacttt ctgtggaacc ataaacactt aataatttaa tgccgagctg ctggcattca
    29101 cgcgccactt ttttggggat tgtggttccg ccgcaaagaa agaaacgcag cgctgacagg
    29161 tccgcgggtt gtttctctag tacattcaaa agatcataga caaacggcgt tgcgcctagc
    29221 atacaggtgc aacgctgctg ctcaagcagc gcgagacacg catcaggagt gaaaatatct
    29281 aacaacacgc tgcgagcacc aattaaaaat ggtgctgtta cgccatgcag aaatcccgtt
    29341 gcgtgaccaa gtggcgcagg catcataaag acatcctgcc aggtcagatt cagccgcacg
    29401 caataagccc gctcactggc gagaatattg ttatgcgtta gcattacgcc ctttggcaga
    29461 ccctcggttc cggaggtaaa cagcaccgca gctaattcat cgccgtgggt cgttatcgcc
    29521 gtggtcagtg aggtattgtc ggcgataatc tgacttaatg agagggaaga ggtggcggga
    29581 gccagtttgt ccacgccgac aatttgttgt agttgtggaa gctgattttg cagcggcagg
    29641 attaaatcta ccgggcgcgt ttgtttaaac aacgtcggtg caaagaacat ttttgcctga
    29701 cacttattga gcacccacac cagttctgct tcccgccagg aaggcaacag cggcacggaa
    29761 accgcaccga ttttcaggca ggcaagatag ataacggtaa attcacacca gccaggcagt
    29821 tgaaatgcga tgcgatcgcc tgattcaata cccttcgcta acatccagtt tgccagacag
    29881 ctcgcggcgt gatcgagcgc gctataggtg tacgatgcac catgattatc gaccacggca
    29941 attttgtctg gcatcgcacg agcggtctgc tgccagtaat cggccagcga agcatcgccc
    30001 catagccctt gctgacgata cgccgcacga cgttgttcgt tgaacgttaa tgtcactttc
    30061 atgttggact ctcgaaacag aacatcaggc ccgagatgcg ggcctgtggg atgcatgatt
    30121 agccgtagcg aaaatcaata ccgaaggtgc ctgcgggata ttgccactgt tcgagaatag
    30181 tgttaccgca cagcacccga caggtgccgc actccagaca accggcagaa tcaaaatgaa
    30241 tgttgccggc gtcatcctgc ttataaagtc cggcagggca ggctttcatt aatttacgga
    30301 attcattgat atcgggattt gccgccagaa tgatatgcgg gtggccctca tcaacatgga
    30361 atttattgac gcctaatttg atgtcgacgt taaccgtagc gttctggctc atagcgcggt
    30421 tgctccctta atgccatctt tcagcaagtt gatcagccca attttcttcg catgtcccat
    30481 gatcattttg cgtaccggct ggtttggttt gccgtcaatg gtgaacatct cgttcatgat
    30541 gtcggcgacc attcgtgggt attggctaaa caggcgcggg ttttccatca gcgccgggat
    30601 cttgcggaaa tgctgcatat cgcgcatgac gcagctttgt tccagctcac gtttgtattg
    30661 cgccagactg ctggcggaga aatcctcgcg ttctttggcg gcgatcaccg ttgtggcggc
    30721 agcctgagcc gatgcaatgg ctaaatccat gccacgaact gtaaaaccca aattcaggca
    30781 gaagcctgcg gcgtcaccaa cgatcatcac gccatcgtta accatctgcg gcaccattgc
    30841 cagaccgcct tctggcacca tatgcccgga atattcaagc agtttgccgc cgctaattag
    30901 tgggcgaatg gcggggtgtt gtttaaaatc ttccagcatt tgcggcacgc ttttttgcgc
    30961 atgggcgata tcacccaatc cacaaaccag ccccaaggat atggaatcct tgttggtata
    31021 gagaaatccc ccgcccatca ggccgtcaga aggggaaccg gcaaacagcc aggcggcacc
    31081 ttcctcgccc gtaatattaa agcgatcgtt gatctgttct ggtgtgaggc caataacctc
    31141 tttaacacca acagcgtaat gatgcggatc ggaagcggga accattccca gcgagcggcc
    31201 aagcatcgag ttaacgccat cagccagaat caccacattc gcttcgagaa tatcatcccc
    31261 agcctgcacg ccagtgacct tgtttccttc acgaaccagc gcatcgacgc gaacgcctgg
    31321 gataaactgc gcgcccgcct gctcggcttg ttccatcaac cacgggtcca gacgattacg
    31381 caatacggta taagatgcgt gttgcggaac atctggttgc tcgcggtgaa aatcgagggt
    31441 aacggcgctt tcttcggtta agaaggagat tttctcgcgt gtgaccttgc gttctaccgg
    31501 cgctgatact gcaaagcctg gaatgattgc ttcaagtgtg tgggcataaa gacgcccgcc
    31561 ggtcatgttt ttacatccgg cactgtcgcc gcgttctatc accaggacat ccagcccggc
    31621 tcgcgccatg accagtgcgg caacgctacc agcaacgccc gcaccgacca caatggcatc
    31681 aaatttgtct tccgacatgc gttatgctcc cttcccgctg cgtttacagc gggagatgtg
    31741 gttgaaaggg gaattagcgg ctcaactggc taatcagggc agggacgact ttgtagatat
    31801 cgcccaccag accgtagtcg gcataattga agattggcgc atttttatct ttattgatgg
    31861 cgacaatcac ttttgcgccg ttgccgccaa ccatatgctg gatctgcccg gagatcccca
    31921 gcgtcaggta gagatcggat ttcagcaaca cgccggagac accgatataa cgttcacgct
    31981 ccatccagtt ctcgccttcg gcaattggac gtgaacagcc gacttcagca ttcagcaccg
    32041 ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc cagaccacga ccgacgccaa
    32101 ccacacgttt tgctttgctc aggtccacgc tgcttgcggc tttcgcacgg cgttcgcgac
    32161 agagaatttc atgacgcgga gcaacataag gtaccgtttc tgtcgggcac tgatgagagg
    32221 tatcactggt gcacggttcc tgaacaccgg gtgcaagggt aatgatcgcc agcgggctgt
    32281 tgatcttttc ctgagcgaac gctaacccgc catacatccg gtgttcggcg caaatgtgac
    32341 catcgataat atccaccgcc gtggcatcgt tcaccagcgc cgcattcagt tgcacactta
    32401 accgtgctgc cagagcttta ccacgtttcg tcgcggccaa cagcaacata gcggggtgtt
    32461 tatctttcag cagggcagca atgctttcgg cgtaattttc agtgcgttgc agcgcgtcgt
    32521 tttgctcaag aacataaata cattttggac cataaggcat aactgcctgc gcctggtcgg
    32581 tattttgtac aatggcatac acctgttggc cccattgctg agcgccgcca aacagttcag
    32641 cataacgttc aggattatcg ctaaagaccc agacgctgtt taattgactc atcgtttctt
    32701 ctccgtaggc ttcagttcag ggctttcttc agatgttccg ccagctcggc aatggcctcc
    32761 ggcgaatcgt tatcgataat gatgtgctta cgttctgttt gcggcggtac gcgaatgccg
    32821 accagttcag caagtggcgc gctctggctc cagtcaatac cacttgcctg ccactgattt
    32881 accggttttt tacccgcgcc gagaatggct ttcatcgaag gaatgcgtgg cacgttaata
    32941 tcggaggtga cgcagagcac ggctggaaca gagagttcaa taacttcaac atcatcttca
    33001 agcgtgcgtt caatcaccag tgtattgccc tgacgctgaa tagcactcac tgcattaatc
    33061 accggaagtt gcagaatttc tccgaccagc aagccagcct gctgggcata aaggtcgccg
    33121 gaaccttcac caaagatcag taaatcgaag ccgatctttt caactgctgc tgccagcgct
    33181 tttgcggtat cgagaggcag tgcatgttca agttgcgcat cctgcaccaa atacaggctg
    33241 tgcggcccgc gggatagcac gtctttgcgc actttcgagt tctgcaacaa tgagccgcca
    33301 acggtcagcg cggctatctc atcgtcatct gttgcaagct ggcttgcagc ttcaatggca
    33361 ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt gtattctgga
    33421 gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat tttcatgatt
    33481 tctcctgtga gtcgcatacg gaaccatctc cgatggaagg taaaaatggc ctgaaaatag
    33541 tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt tttccttgcc aacaatcttg
    33601 caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga
    33661 ccgtacaatc ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta
    33721 taaccgcaat acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata
    33781 gggaggttgt catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg
    33841 gtaaaacgcc acggctttca cgttttgcaa ttagcgatga tccaaaatgg gagtctggac
    33901 atcacgttca tgacaatgaa accgagctga tttacgtcaa gaaaggggta gcaaggttta
    33961 ccatcgattc ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca
    34021 ggctgcatgc ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt
    34081 acggctttca gtttcagggg gctgaggaaa atcagctact gcaaccgcat tcttgtccgg
    34141 taattgccgc ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga
    34201 ttttgccgca aagtaaaaat agccaaacat cttcgttata ggatgcattt gcctatacgt
    34261 tagccattct ttactacgaa aattttaaaa at
//
LOCUS       DAZFYC010000041        33608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFYC010000041 DAZFYC010000000
VERSION     DAZFYC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.041"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(209..2302)
                     /locus_tag="ACHKW5_002215"
     CDS             complement(209..2302)
                     /locus_tag="ACHKW5_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7511608.1"
                     /translation="MTVSTEVDHNEYTGNGVTTTFPYTFRIFQKSDLVVQVVDLNENI
                     TELILDTDYIVTGAGGYNGGNIILSKALVSGYQISISRELPFTQETDLRNQGKFFAEV
                     HEDAFDKLTMLIQQVRSWFSLALRKPSFVANYYDALNNYIRNLRDPSTPQDAATKNYV
                     DTQLSSNLGCTLRVQEQIPQLPDAASRANKMPAFDNDGNPIVVLPPSGSASDVLIELA
                     KPTGSDLVGYGSQTVKATLDSLNSMREELLDKTGFNALGRFLNLSELRSCVPEEAGQI
                     VYVASAASTTHAENHLGGGFFESVDNIQAWADDGGIVIKPETGTMVWRRINFTTYDMQ
                     FWGVKPDGVTDNATAITLATNFARSNKCILEAPAGNINTSKTIPIYDNMGIRGQGKAE
                     ATVFYKTTNDKIDLTKNGEVILQVDALCAFIPKQWDLTDNSMSSFCVNGRVENCMFRR
                     LGLTQENVDSYRNYYGLFLGKSAAPVIRQSIFECAYIGCFSYAPFSGVMEMVGFPQYP
                     GKGYAGVLFEDFRDGQIKVIGTSMDMRLVQVNGYQLSFRMSGMQYTTMTNCTAENCTP
                     MDGESTCYAFDFVNPYCIVMNTCATEFVKGGQLRVSVQGNPSFRPSIIVNGFLPIDQQ
                     SPVVQTPIIDIDNGGVVEMSVILNGGDWAINPSAVNLTSPKASGNGLKVRLIGVNGSP
                     SSVWSASSGAEVREF"
     gene            2498..2755
                     /locus_tag="ACHKW5_002216"
     CDS             2498..2755
                     /locus_tag="ACHKW5_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511609.1"
                     /translation="MQQYHYPLEDGFTERIHTPGGVRSLVEGSHLMKLLRDLDKDGFN
                     VDGPLAELTALINYVTSSQMSMRDLQTHLDYCAEQLRKQTR"
     gene            3068..3754
                     /locus_tag="ACHKW5_002217"
     CDS             3068..3754
                     /locus_tag="ACHKW5_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO7511610.1"
                     /translation="MTANVTPSVFHFESEATIRAIVIDGNPWFVAKDVIKALQLTNPT
                     MSIKSLDDDERAKFNLGRQGETNIINESGLYTLILRCRDAVTPGTIPYRFRKWVTGEV
                     LPQIRRTGSYIKNSLPQEERIKMVADQVANATASAVMKAMKIENKTYSAPLKPGYRSL
                     IHSPSGVLGLTENSLLMNLLNQLQDDGHDVSGAAAELTTMFCYIVGVSKCLRDIQTHA
                     EYINDKAGFF"
     gene            3894..4526
                     /locus_tag="ACHKW5_002218"
     CDS             3894..4526
                     /locus_tag="ACHKW5_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511611.1"
                     /translation="MRTNTTRKAMPYIIPEADFDRKLNMSEKNTSHTESYLAKGVVDF
                     VLPGFTTPYGYRLVKSCNGDHYRMITTSDTPETVYAVKLIFREDIVEAKRTCTQIMVW
                     RTPNVIHDRAVHGLPQIFFQFFLEQYAIVVSDEQQTIDGRRFWERMISWAINTPGYNV
                     YVSDGSEEDRPLSFMTSWDDFYSQWADFCWGSDKDVHTHRLLVISKDKLH"
     gene            4625..4786
                     /locus_tag="ACHKW5_002219"
     CDS             4625..4786
                     /locus_tag="ACHKW5_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="HFO7511612.1"
                     /translation="MKKAFAALFVLLSLVASTQAFAGRCQHDSDTAADGSRCGGRSAD
                     SRPGGGGIR"
     gene            complement(4859..5515)
                     /locus_tag="ACHKW5_002220"
     CDS             complement(4859..5515)
                     /locus_tag="ACHKW5_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO7511613.1"
                     /translation="MNAQLIPVFNGTISNETALLCNARDLHAFLGVKKVFAAWITNRI
                     SEYEFIENQDYILLSNLGKQTSGRGGHNRKEYHLTLDTAKELAMVERNEKGRQVRRYF
                     IECEKRLRQQETKVEKVLSGFMPAIMEAIKLEDKKEYSAPLKPGYRSLIHSPSGVLGL
                     TENSLLMNLLNQLQEDGHDVSGAAAELTTMFCYIVGVSKCLRDIQTHAEYINDKAGFF
                     "
     gene            5950..6216
                     /locus_tag="ACHKW5_002221"
                     /pseudo
     CDS             5950..6216
                     /locus_tag="ACHKW5_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
     gene            complement(6430..6594)
                     /locus_tag="ACHKW5_002222"
     CDS             complement(6430..6594)
                     /locus_tag="ACHKW5_002222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511614.1"
                     /translation="MDIKEFSELERRYEQAKTNRAIGAFIGGGGVFALLADFLWDVAN
                     PISIGVLIGG"
     gene            complement(6613..9636)
                     /locus_tag="ACHKW5_002223"
     CDS             complement(6613..9636)
                     /locus_tag="ACHKW5_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511615.1"
                     /translation="MPFYPVSESNNNGFISAGLAIPEPDEQSFDVHPPEGKNPEPRPK
                     EPSLLAAAIRQNNILAGFFRPARQFEPVEGYNPYADKNELHGYEYWGAKFAGSRSPEE
                     TAWIKQQIDDENEDRRFISEAGLAGGIASATAMLFDPVTVASMFIPGAQGGALARIGS
                     QIAIGAAGTALSEVVLNNQQITRSWGESAAHVAAGAMMSGVFASAGVALSPSVRAAAT
                     REVADALDNMSITSATDRAAASLSDGGSVGAMKIDTATLDDLTPVSGGVVGKAAWKAG
                     SYLTPLTRLMESPSKTVRKTTLELAENNFTLKGNERGIETPVAVETRTRGWQREEAAV
                     VVGNKQAYAKYKADGGDMSFDSFRQQVGNAMRSGDVHANPVVQETAQAMRTVLNRVKV
                     EMQKLGLLPPDEELKALGQASYFPRIYKVGKIISERDKFRRILVDWWSRGNKTLDPED
                     AEIAADIVINKITGAKVPQDFVSVFSVKAAGSTKERTLNVPDSLIRDYLESDVNYVLQ
                     RHIREAAAEIELTRTFGKRTMTERLQLIEDEYDSLLREVPEKIKAKYDESVANLKARY
                     ESNGEVVPQGKLDSLMRKYEKELRKEQSRLSKSRANDLRDITALRDRLVGTYGMPDDP
                     SSFFVRAGAFLRDVNFTTKLGGMTVSAIPDLARGVMVNGFRNTMKGYASQISQSPAFK
                     ASKEEMLKMGIGLEAVLHSRSRAIGDLVDSSSRTTAVEAGMERITDAFGKLTLMDRFN
                     DINKSMNGMLTSDGILSGAFSARRMAKLGINDNMAARIRSEFEKHGEVIDGWHIGNFD
                     KWDDQYVAGVFQSAVLKDVNNTIITPGIGDTPLWASTPMGRTIFQFKSFTTASYNRAL
                     LGGLQEGTAQFYYGTAFQIALGSLVYALKEASKGKNVDWSPEKLVLEGIDRSGILGPL
                     MEYNNMAEKATGGAVGLGALFGTGTQSRYASRGFVGSLFGPSFGLADSIIDVTAGVLN
                     GDAGDRIVHNVRTLIPGNNLFWIAPLINQVDPVMK"
     gene            complement(9636..12344)
                     /locus_tag="ACHKW5_002224"
     CDS             complement(9636..12344)
                     /locus_tag="ACHKW5_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7511616.1"
                     /translation="MPVVPTVSGRQVQSRGVQTGGFQTFDVPQAGQVLANVADQYAVA
                     YGEARKKANVALSQDAILQLNQRSNERLYNPQTGFYAQQGKNAIGKGQEYISGFDQDV
                     EEIAASLTDEAARNMFLQQARTQKIQFSTGVLRHEIGQTNAYEDEQYQATRKLWIQNE
                     ADAWNDPQTATLARNSRMVAIARYGAARGWSQERILEEIESDDRRATEMRAKNYAAAN
                     PEGWLNGLFQKNDSGGMDMRAIRLVESGDRHFNPDGSLLEGPITSSGERAQGKYQLMP
                     GTGKELAAKRGVKYNPTDEQQHEMLASDYVNQLYGKYGSEILTGAAYNWGMGNVDKLI
                     AKVGDPRKGEISEEEFIRNLPSETQGWLSRYRKNKTGMDPLTIYQIDNLANSQIEKQR
                     KLILEQLEPAINNTMAQLYNGEVPDYIPAQETIIRGYGKNADKIINQLDIAIDNARIF
                     QAIQYLPPSQQQEEMQKVKPEVNDPHYALKLDAYGKLSALLQRSNEAIQAQRDSRRFN
                     EALTIGEKLDPSNKSMQKAADYTEMAQNFRINDASTHDGVVRLVAQTGIMPSQVITQL
                     SAVSRSSNQEVVKNAANLFSRLYETDNASIGNMPKDMQGFYLTVKQLTDAGMSSDAAI
                     VEAQSKTYNQTDALRAQLSSVQSTREYKKERDSAANSAVSNMAHWLRWDPSADDQTPE
                     AALFRNDYQMLYDVNYRLAGGNADVAKQMTNQQIARTWSISEVNGEAQFMKYAPEALY
                     QYGPSGWIAAQWKAEKEKIMYGDDLHKLSPSFNRPSTEAGALGLNTPRSLVGGELILV
                     PDLSTPRDKLYSVVIRTKDKDGITRDDLFYDKHGRQMRWGPSLEDWEPYKKMQQEREQ
                     HEQEEIMRGQAIQNFKDKHRALDEQYQRLHNERMDKFKDYFSWGSK"
     gene            complement(12344..12889)
                     /locus_tag="ACHKW5_002225"
     CDS             complement(12344..12889)
                     /locus_tag="ACHKW5_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511617.1"
                     /translation="MCDPVIAGGAMLAMSGIQAYTQYQQGKYASKVAEANADIATAQA
                     NDAINRGNAEAEQRRRETRQRLGTQAATMGATGADLSTGNALDIFGDTAQFGALDSLT
                     TVNNAQREAYGYQVQAANYKAEASSARKQGNVGAATTLLTAPLKAYGAYQMFGGTWSP
                     FTQSTPAPIGAAAGTRLPGGL"
     gene            complement(12889..13353)
                     /locus_tag="ACHKW5_002226"
     CDS             complement(12889..13353)
                     /locus_tag="ACHKW5_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511618.1"
                     /translation="MIDVQIIPATEEHLQMILPDVRQADIDELYAVSLMTTEDALRVG
                     LRTATMAWSGFANGELVTMFGVSPASMIGGNGTPWLVGTSRIEKYQKTFLRHCRPVLQ
                     QMLAVYPRLENYVDERNHVAKAWLHWLGFRLEEAAPYGALGLNFHRFHMERK"
     gene            complement(13353..15824)
                     /locus_tag="ACHKW5_002227"
     CDS             complement(13353..15824)
                     /locus_tag="ACHKW5_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511619.1"
                     /translation="MAISWIQPSFAGGEIGPSLYGRIDMAKYQVALRKCDNFIVRQYG
                     GVENRPGTRFVGAAKYSNRKCRLIPFQFSTVQTYALEFGHQYMRVIKDGALVLNSSNV
                     IYEIATPYTEADLFRIKFTQSADVLTLVHPAYPPKELRRYAHDNWQLVDVVTKNGPFE
                     DINIDESVTVYASASTGTITLTANASIFGAEQVGKLFYLEQPAVDSVPVWETSKSTSI
                     GDIRRADSNYYRAVTAGKTGTLRPSHTEGTSWDGWGGSGDDDTGIEWEYLHSGFGIAR
                     ITAANGTTATAEVISYIPSQVVGEDNASYKWAKYAWNSVNGYPGTVVYYQQRLYFAAS
                     TAFPQTIWASRTGDYKDFGKSNPTQDDDRIIYTYAGRQVNEIRHLIDVGSLVALTSGG
                     EYVITGDQNKVLTPSSFAFSSQGSNGSSNVPPIAVANIALFVQEKGSVVRDLAYSFDV
                     DGYQGNDLTILANHLFQKHSIVDWCFSIVPYSSAFCIRDDGKLLVMTYLRDQRVFAWA
                     PQSSTGKYESTCSISEGNEDAVYFVINRTVNGQTVRYIERLSSRLFTSDEDAFFVDSG
                     LSYDGRNTSDRTMTITGGSGEWDYRAEYTISVSGGAYFTSSDVGAQLQFPYTGTDPDT
                     GDEVSKELRCDIISVTSNTAVVVRANRNVPPSLRNVATTNWQMARRTFGGLSHLEGQT
                     VNILSDANVEPQKVVSGGAVTLESPGAVVHIGLPITAEFETLDININGQETLLDKKQV
                     IPSVTLVVNASRGIWATTPGGKWYEYPQREFEFYDDPVDDATGKVEVKLDSNWGKNGR
                     VKIRQLDPLPLSVLAVIPRLTVGGF"
     gene            complement(15824..16429)
                     /locus_tag="ACHKW5_002228"
     CDS             complement(15824..16429)
                     /locus_tag="ACHKW5_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511620.1"
                     /translation="MASVVEICNRALSNIGNSRSINSLTEASKEAGECSLHFEACRDA
                     VLSDFDWNFATKRVALADTSNPPPDWEYAYQYPSDCLRITEIMLPGVRNPTAAMRVQY
                     EVGADTNGTGKLIYTDQPQAWLKYVSRVSDVNMFDAIFMEALAWRLAAAINMALTGNA
                     DLGTFALNMYNRVILSAGSHSQNESQEPQPPVDEFTIARLS"
     gene            complement(16429..16752)
                     /gene="gp10"
                     /locus_tag="ACHKW5_002229"
     CDS             complement(16429..16752)
                     /gene="gp10"
                     /locus_tag="ACHKW5_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid staple protein"
                     /protein_id="HFO7511621.1"
                     /translation="MELVNLKTGTDSYQDESGETRTRDEYPWGLCITLNNDTLNKLKA
                     QPKGVGTEVMITAKAVIRGLSARETDDGVNRSADLQITDMAIAPVSGGVEKSAAETLY
                     GNGGE"
     gene            complement(16803..17138)
                     /locus_tag="ACHKW5_002230"
     CDS             complement(16803..17138)
                     /locus_tag="ACHKW5_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511622.1"
                     /translation="MSGEKPRYRVLRLSHIHNTLWPEGAEIEYEGEPGSALEPVNDAA
                     RQAKAKVAGKVSMAATSTKIINDVSDDGELDKLREEYELLFNEKPHHNAKAETLREKI
                     ADKRKELGV"
     gene            complement(17149..17586)
                     /locus_tag="ACHKW5_002231"
     CDS             complement(17149..17586)
                     /locus_tag="ACHKW5_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bbp16 family capsid cement protein"
                     /protein_id="HFO7511623.1"
                     /translation="MITDKLLMFSEAQAVTNTAASTDVIDLGPIDGKRRDIGVGYPLE
                     FWALVNTAAAASGDATVNIQLQTSENNSSWTTIYDSGALAKTALTAGKRVVSAKVPAG
                     VQRYLRVNYSVATGPLTAGEFTAGISLDVDANTPYPIRSKVTG"
     gene            complement(17638..18624)
                     /locus_tag="ACHKW5_002232"
     CDS             complement(17638..18624)
                     /locus_tag="ACHKW5_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7511624.1"
                     /translation="MAVKGLTALTLADWGKRVDPNGKVDKIIELLGQTNPILQDMPFV
                     EGNLPTGHRTTIRSGLPSATWRLLNYGVQPSKSTTVQVTDSVGMLETYAEVDKSLADL
                     NGNTAEFRLSEDRAFIEAMNQQMAQTLFYGDSSVNPQQFMGLSSRYSSLSAGNAQNII
                     DAGGTGTDNTSIWLVVWGENTVHGIFPKGQKAGIQMEDKGQVTLEDANGGKYEGYRTH
                     YKWDNGLALRDWRYVVRIANIDVSNLSEPSSAANIAKLMVKALHRIPNRGMGRPVFYM
                     NRTVGQALDLQSLEKTSLAISVKETEGEWWTSFRGVPIRETDALLETEARVV"
     gene            complement(18639..19334)
                     /locus_tag="ACHKW5_002233"
     CDS             complement(18639..19334)
                     /locus_tag="ACHKW5_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO7511625.1"
                     /translation="MNLFERLLYSRLCNEQPVDGGAAPAASEPSAPAGDNPAPVGDPS
                     QQEGDKPQPVADGDKPAADKKPENDKLDEKKDGDKPEGAPEKYEFQAAEGVELDTEAL
                     KEFEPVARELNLTNEQAQKLVDAYPKILAGVQQRQAEAWQKTTEQWAADVKADKEIGG
                     DKLISNLSAAQRALDQFGTPELKEYLNTTGLGNHPDLVKTFVKIGKAMSEDGMVTGGN
                     EGQRSAAEVLYGK"
     gene            complement(19337..19633)
                     /locus_tag="ACHKW5_002234"
     CDS             complement(19337..19633)
                     /locus_tag="ACHKW5_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511626.1"
                     /translation="MTDYEDDQLKEENARKQRDMAQREIDDIRFVMSCEQGRRVVWSV
                     LEKGRVFSAISPMDAMAMAFNEGQRNLALELFQRVMAHCPEQYLKMAKEASEQE"
     gene            complement(19630..21309)
                     /locus_tag="ACHKW5_002235"
     CDS             complement(19630..21309)
                     /locus_tag="ACHKW5_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO7511627.1"
                     /translation="MAETTKERLNKQFAQLESERQSFEPHWRELSDYINPRGSRFLTS
                     EANRNDRRNTRIIDSTGTMAARTLASGMMSGITSPARPWFRLATPDPEMMDYGPVKLW
                     LEAVQNRMNDMFNKSNLYQSLPQLYGSLGTYSTGAMAVLEDDEDIIRTMPFPIGSYYL
                     ANSPRGSVDTCFRKFSMTVRQLVQEFGLNNVSESVKSMWESGTYEKWIDVMHSVYPNI
                     DRDTSKLDSKNKPFKSVYYEVGGDNDKLLRESGFDEFPIMAPRWEVNGEDVYGSSCPG
                     MLALGPVKALQLLQKRKSQLIDKATNPPMVAPTSLKNQRASLLPGDITYIDQITGQDG
                     FRPAYLVNPSTADLVADIQDTRQIINSAYFVDLFMMLQNINTRSMPVEAVIEMKEEKL
                     LMLGPVLERLNDECLNPLIDRAFSMMVRKNMLPPPPDAMEGMPLKVEYISVMAQAQKS
                     IGLSSLASTVNFIGQLAQAKPEALDKLNVDQAIDAFADMSGVSPTVIVPQEQVEQARQ
                     QRAQQQQQQQMMAMGMAAAQGAKTLSEAKTSDPSVLSAMANAVSGQGGQSQ"
     gene            complement(21324..21530)
                     /locus_tag="ACHKW5_002236"
     CDS             complement(21324..21530)
                     /locus_tag="ACHKW5_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511628.1"
                     /translation="MCMGSSPSVPATPEVQAAPQEQDAAVVDARDEETRRRRAAAGRS
                     STLLTGSQGDTSTANTSGKTLLGQ"
     gene            21958..22034
                     /locus_tag="ACHKW5_002237"
     tRNA            21958..22034
                     /locus_tag="ACHKW5_002237"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21992..21994,aa:Arg,seq:tct)
     gene            22420..23160
                     /locus_tag="ACHKW5_002238"
     CDS             22420..23160
                     /locus_tag="ACHKW5_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511629.1"
                     /translation="MLESLKEYLSSTVNTASQRVRNPVFGAFALSWCAFNWKSILYLL
                     LSDSSIIDKITYISSNSSWKTVLAFPCVSAIILCGGMPWINNVISKWQAKPLDNYDSI
                     ENHRKAKRIHRATRLQRLKAKHDVTYDKVKTGAEKDIQAMKERITESQARMGELTSER
                     DRLSDKVLELNKKLENMQSVAAALKIEAEKSARMLEDREAKINNLIQELKEKEKESIV
                     LHMGHEPLRKTYTSQAERMIAEAVKNKQ"
     gene            complement(23162..24637)
                     /locus_tag="ACHKW5_002239"
     CDS             complement(23162..24637)
                     /locus_tag="ACHKW5_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7511630.1"
                     /translation="MTDTALSPEEQLIEDIAGFTHDPLGYALYAFPWGEEGTELAHAT
                     GPRQWQADAFREIRDHLQNPETRYQPLMLARASGHGIGKSAFISMLINWGMSTCEDCK
                     VVVTANTDNQLRTKTWPEIIKWSNLAITKDWFTCTATAMYSNDPGHDKRWRADAIPWS
                     EHNTEAFAGLHNERKRIIVVFDEASNIADLVWEVAEGALTDEDTEIIWVAFGNPTRNT
                     GRFRECFRKYKHRWKTAQIDSRTVEGTNKQQLQKWVDDYGEDSDFVKIRVRGIFPDAS
                     ELQFIPTGLTDEAMKRVVTAAQVAHAPVIIGVDPAYSGVDDAVIYLRQGLHSKVLWTG
                     NKTTDDLIMAKRIADFEDKYQADAVFIDFGYGTGLKSIGDGWGRTWQLVPFGGASTDP
                     QMLNKRGEMFNSCKTWLRLGGMLDDQETADDLSTAEYKVRVDGKIVIEPKEDIKERLG
                     RSPGKGDALLLTFAFPVSKRLRIPGQQNQQGKAITDYDPFA"
     gene            complement(24634..25308)
                     /locus_tag="ACHKW5_002240"
     CDS             complement(24634..25308)
                     /locus_tag="ACHKW5_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7511631.1"
                     /translation="MNTAANYGGFILHVTITTTVTITTVVMMPAEPKTYKRKSTQFKP
                     LTAMQEAYCQSYIKTPENQTQAAINAGFSPNTAAVKASVMMRDERIQKRIAELMEERN
                     KRMRVSADYVLMRLVEIDQMDVIDILNDDGSLKPIREWPKIWRTTLSGFDLSSTIMNM
                     NEDSIETILKKIKWPDKVKNLELIGKHVDVNAFKERLDVNVNVTIADRIAAARKRLKE
                     RQDGNQ"
     gene            complement(25305..25517)
                     /locus_tag="ACHKW5_002241"
     CDS             complement(25305..25517)
                     /locus_tag="ACHKW5_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511632.1"
                     /translation="MPKIRYDLEDMRDNSANFPKEVKFLMHKYGCARRDIVIDSQHPC
                     GEDVIFIRGKWEGYLDESFYDEFDGL"
     gene            complement(25534..25872)
                     /locus_tag="ACHKW5_002242"
     CDS             complement(25534..25872)
                     /locus_tag="ACHKW5_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511633.1"
                     /translation="MAKSAAERKAAQRARQAESGVRKLEIVLDAQEIEMLERNCATRR
                     PGRAPYEFGEYIALLIRQDDARVRGRIKSISRKRCGKCGERVPVNSCPCNGDSQCWVT
                     KGWHETKLIV"
     gene            complement(25865..26146)
                     /locus_tag="ACHKW5_002243"
     CDS             complement(25865..26146)
                     /locus_tag="ACHKW5_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO7511634.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDIPYDGYEIKTITHPHFGDKPVKVFAIKVNIDG"
     gene            complement(26139..26423)
                     /locus_tag="ACHKW5_002244"
     CDS             complement(26139..26423)
                     /locus_tag="ACHKW5_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO7511635.1"
                     /translation="MDSFAKYTIIDWMAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGSVIVVTTQSGMTIQIHKPKEEGRG"
     gene            complement(26423..26587)
                     /locus_tag="ACHKW5_002245"
     CDS             complement(26423..26587)
                     /locus_tag="ACHKW5_002245"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7511636.1"
                     /translation="MPAQDDWILIYSKHGEYMAGQVQGEYVELSDGTLSWLGNVLFWM
                     PLPEPPQEVN"
     gene            complement(26699..27010)
                     /locus_tag="ACHKW5_002246"
                     /pseudo
     CDS             complement(26699..27010)
                     /locus_tag="ACHKW5_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(26992..27648)
                     /locus_tag="ACHKW5_002247"
     CDS             complement(26992..27648)
                     /locus_tag="ACHKW5_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eae-like domain protein"
                     /protein_id="HFO7511637.1"
                     /translation="MDCNIKGLVTAGHEMASELKAECGAVDMRSVAKLISDLATQLEV
                     QLVRANALAEDQQKAIESIKQADAAVKLAHEKFSELAAENAALKAFKTAVYQQMGVGC
                     DAPEFSITVGLSNLRRFADTLHAIEREFFTKELPDEEHEGETFNECPLSWGMSVEQYV
                     SEFRKCLAEVRAQGLEMFAQKCNSKSEQSFASDIRDNWKLLGEHATDFAAELRKGGNQ
                     "
     gene            complement(28315..28668)
                     /locus_tag="ACHKW5_002248"
     CDS             complement(28315..28668)
                     /locus_tag="ACHKW5_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFO7511638.1"
                     /translation="MRKQIQALGRLKTGQMNKTESAYCQHLEQRKRAGEVAWYRFEGI
                     KLRLADNCFLTVDFAVMLADGQLVMVDVKGSKSVFTDDARVKMKVAADSYPFVFQVAY
                     PKPKKLGGGWEVEEL"
     gene            complement(28786..29571)
                     /locus_tag="ACHKW5_002249"
     CDS             complement(28786..29571)
                     /locus_tag="ACHKW5_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7511639.1"
                     /translation="MNNVFTAIQNRDGEALSRMSGYEHQYTNNDNVVNMSAERLVDAL
                     FKQLKQLFPAAVVTNLKTPEQEVAAKQQWIAAFAEGGIRTREQVSAGMRHARASESPF
                     WPSPGQFIKWCKDSKMVLGVTIDDVMAEFHRYSKEKSLYPGGPERFPWRHPVMYWVVC
                     DTRRAMYQRQLSEIEVEKHARRLLDDWAKKVASGQQIPDPVISIQAKPEPMSTPPDTG
                     RDVYHPPGRSFGCMPNAATLGGITPAQWLMEEYRRGKAAGFIK"
     gene            complement(29568..30383)
                     /locus_tag="ACHKW5_002250"
     CDS             complement(29568..30383)
                     /locus_tag="ACHKW5_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO7511640.1"
                     /translation="MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLV
                     KDTCLNKKTVQSGLISLMKMGLISDTGERKGATKRVRVFSLNITKNGNIKGNREGGNE
                     PENGNVTENGNIPKNGMLNDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKTGDSVPH
                     DPGANNAVMNNFVPPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAE
                     FRIYWMAEGKAYHQAQWEQKLARRLQISRQKQSTLPDNNVPHWNSPEAWEDFL"
     gene            complement(30399..30599)
                     /locus_tag="ACHKW5_002251"
     CDS             complement(30399..30599)
                     /locus_tag="ACHKW5_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511641.1"
                     /translation="MEIITRIDAAKRGLKRYYTGKSCKHGHDSERWVYNGHCVECTME
                     SNRRIRAEIKQIMINSSPQHSS"
     gene            complement(30750..30980)
                     /locus_tag="ACHKW5_002252"
     CDS             complement(30750..30980)
                     /locus_tag="ACHKW5_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511642.1"
                     /translation="MSDNGHFDFKKHWLALTPDEREAFAQEAGTTSHYIQTHLTGKRK
                     MPGKVLMNGLFKACKTRQWLRSKAELAYFFYS"
     gene            31135..31719
                     /locus_tag="ACHKW5_002253"
     CDS             31135..31719
                     /locus_tag="ACHKW5_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7511643.1"
                     /translation="MKSIQDVRRQNLNDLIDREFNGVQTRMAEKLGTQANLVNRWALG
                     KKVIGDQVARKIEAAANKPRNWLDIDRSLSQEGFQPVGPSDIGQLAAHNLERWMSESR
                     DLSTQGKLHRASGVAQVTISRLLNNEVSVSISTLENVASAFGRHGYELLIHPHDPATI
                     NYDRSRYALLPETEKAKIESYIEFVINQNEKNKQ"
     gene            31873..32025
                     /locus_tag="ACHKW5_002254"
     CDS             31873..32025
                     /locus_tag="ACHKW5_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511644.1"
                     /translation="MQWKIINGWYCVTACGFMSWKFRTLQEGIKWAFVSKEARDVAND
                     NEIWEG"
     gene            32028..32327
                     /locus_tag="ACHKW5_002255"
     CDS             32028..32327
                     /locus_tag="ACHKW5_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511645.1"
                     /translation="MNVNQQKNLQKIMLAFDKDYRLSEQLYDRQVELIESIRLHQLAS
                     TFDVVTGKGVRQEVLEAAKDSPEFEELMDAYRREAMAIIARWDLADRIDGQREAA"
     gene            32324..33223
                     /locus_tag="ACHKW5_002256"
     CDS             32324..33223
                     /locus_tag="ACHKW5_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFO7511646.1"
                     /translation="MMQNAGIMDRTKYIGGSDVAGILGISPWRTPLEVYLDKVKPRVK
                     PVDPSKQKVFTRGQRMEPYVIDLLSEETGMEIVHRGNRYIHRDYDFIAAEIDAEAASG
                     ENIEIKTVSPFKAKEWGEIQTDAIPVHYTAQAMHGLMVTNKQACVFGVLIGGDDFRIY
                     RVERDEETIQAILEKEIAFWDRVKNLNPPEATSVSDVSLMFEKDAGTSIEADGKALAL
                     FNDLRDMKSRRKSLEEEIAISEEKLKMYMQEHSVLTLDGKPLCTWKSQISNRFDQKLF
                     QSVHPELFEKFKTTTTQRVFRMK"
     gene            33233..>33608
                     /locus_tag="ACHKW5_002257"
     CDS             33233..>33608
                     /locus_tag="ACHKW5_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO7511647.1"
                     /translation="MSINALKAAATGNQVAHHNEKPTTLAGLLADPKIKAQMALALPK
                     HMTADRLARIATTEIRKVPKLASCDQASFLGAIMQCAQLGLEPGGALGHAYLIPFDKR
                     QKVNGRWETVSTEAQLIIGYRGM"
BASE COUNT         8466 a         8627 c         7508 g         9007 t
ORIGIN      
        1 aaaaaaatac tttgccgcag tattaaacac accaattgat gattgagaca atagcccaag
       61 gtgactaaca aaaacaccaa gagctgcaaa cattctcaac gatgtaagac tttgaattat
      121 attaccttta ttattcataa ctctgccgga atggttgaaa tttccggcag attagattaa
      181 catattaatt atgtcaatgt ttgtatagtt agaactccct tacttctgca ccagaagacg
      241 cactccacac cgaagaagga gagccattta caccgatcaa tcttaccttc aacccattac
      301 cacttgcttt tggagaagtt aaatttacag ccgatggatt tattgcccaa tcaccaccat
      361 tcaatatgac actcatttca acaacaccac cattgtcaat atcaattata ggtgtttgca
      421 ctactggact ttgttggtca attggtagaa agccattgac aataatagat gggcggaatg
      481 atgggtttcc ctgaacgcta acacgcaatt gccccccttt aacaaattct gttgcacaag
      541 tattcatgac aatgcaatac ggatttacaa aatcaaatgc gtagcaagtt gattccccat
      601 ccattggcgt gcagttttca gctgtacaat tggtcatggt agtatattgc atcccggaca
      661 ttctaaatga caactgataa ccattaactt gaacaaggcg catatccata gaagtgccaa
      721 ttacttttat ttgaccatcc ctaaaatctt caaacaaaac tccagcatat cctttccctg
      781 gatattgtgg gaaaccgacc atttccatta cgccagagaa cggcgcataa gagaagcatc
      841 cgatataagc acattcaaaa attgactgtc tgataactgg tgctgctgat tttcctaaaa
      901 acagtccgta ataatttcta taagaatcta cattttcttg tgttaagcca agccttctaa
      961 acatgcagtt ttcaactcta ccgttaacac aaaatgaact cattgagtta tctgttagat
     1021 cccattgctt tggtataaaa gcacataatg catccacctg aagaattact tctccatttt
     1081 ttgtgagatc tattttgtca tttgttgttt tataaaaaac tgtcgcttca gcctttccct
     1141 gtcctctaat tcccatattg tcataaattg ggattgtttt tgatgtattt atgtttccag
     1201 caggagcctc tagaatgcat ttgtttgagc gagcaaagtt agttgcaaga gttatagctg
     1261 ttgcgttatc tgtgactccg tcaggtttca ccccccaaaa ttgcatgtcg taagtggtaa
     1321 aattaattcg tctccatacc atcgttccag tttctggctt gattacaatt ccaccatcat
     1381 cagcccacgc ctgaatgtta tcaacagact cgaaaaagcc gccaccaagg tggttttcag
     1441 catgtgtagt gctagctgct gatgctacgt atacaatctg ccccgcctcc tccggaacac
     1501 aggagcgaag ctctgaaaga ttaaggaagc gccccagcgc attaaatcct gttttatcaa
     1561 gaagctcctc ccgcatgcta ttcaggctat caagggttgc tttaaccgtc tgtgacccat
     1621 aaccaaccaa gtcagatcca gttggcttgg ctagctcgat caatacgtca gacgcagacc
     1681 ctgatggagg aagcacaaca atagggttac catcgttatc gaaagcaggc atcttgtttg
     1741 cgcgggaggc agcatcagga agttgcggta tctgctcctg aacgcgcaaa gtacacccca
     1801 ggttagaact gagctgtgta tcaacataat ttttagtggc tgcatcttgg ggggttgaag
     1861 gatcgcgtaa gtttctgatg taattgttaa gcgcatcata ataatttgcc acgaatgacg
     1921 gcttacgcag cgccaagcta aaccaacttc gaacctgctg gatcagcatc gttagcttat
     1981 caaaagcatc ctcatgcact tctgcaaaga acttcccctg attacgcaaa tcagtttcct
     2041 gcgtaaacgg gagctctcgt gatatagaaa tctgataacc gctaaccaat gccttcgaca
     2101 gaattatatt gccaccattg tatcctccag ccccagtaac tatgtaatca gtgtcaagaa
     2161 tcagctctgt gatgttctcg ttcaggtcaa caacctgcac tactaaatca gatttctgga
     2221 aaatcctgaa ggtataaggg aatgtcgttg taacaccgtt accggtgtat tcgttgtggt
     2281 caacttcggt tgagaccgtc atgttaaatc tccggatagt cgcagcaccc gttgcgccgc
     2341 atatccggtt attctattac ctgaaaaacc acatatggat atataaccca taaatacgaa
     2401 cagatattac ctttcaggtg attcgcaaaa cgtgctggat agcaaacaaa ttatttgata
     2461 ctgtatattt atacagttat tgcatggaga agataagatg cagcagtatc actatccact
     2521 ggaagacgga tttaccgaaa ggattcacac gccgggaggc gtcaggtcac tggtggaggg
     2581 atcgcacttg atgaaattac tccgggatct cgataaggat ggatttaatg tcgatggccc
     2641 acttgccgaa ctgactgcac tgattaacta cgtcaccagc tcacagatgt ctatgcggga
     2701 tctgcaaaca catctcgact attgtgccga acaattacga aaacaaacca gataaggttt
     2761 gcaattacca agtggagtgc ttatatttac ctttgcggta aatttacatc gcactcctct
     2821 tgtgccatag taatcgggca ctggcaaaat ccagtgccgg gattggcgtc ccggattact
     2881 acagaggcac atatgccgca taagcggttt ttttatgtgt aaaacgcacc tattctatgg
     2941 tgggctgtgt gggggcaccg aaaggtgcgc cgggtccttt gtagccggtt acgccaaccc
     3001 tgcacagttc accaccaacc gattggcgtc ggtagtggtg attaacctaa ctacaaaggt
     3061 gatcactatg actgctaacg taaccccatc tgtttttcat tttgaatcag aagcaaccat
     3121 tcgagccatt gttattgatg gaaatccttg gtttgttgcc aaagacgtta ttaaagctct
     3181 tcaactgaca aaccccacta tgtcaataaa atctcttgat gatgatgaaa gggctaaatt
     3241 taacttaggc cgtcaaggcg aaaccaatat tatcaacgag tcaggcctct acacactgat
     3301 cctccgctgc cgcgatgcgg tgacaccagg cactatccca taccgctttc gtaaatgggt
     3361 tacaggtgag gttcttcctc agatccgccg caccggaagt tacattaaaa actcgctccc
     3421 gcaggaagaa cgcataaaga tggttgccga ccaggtagcc aacgccacgg cgtcagcagt
     3481 gatgaaggcg atgaagatag agaacaaaac ctacagcgcc ccgctgaagc caggctaccg
     3541 cagcctgatt cactcgccgt ctggtgttct cggcctgacg gagaactcac tgctgatgaa
     3601 tctgctgaac cagttacagg acgatgggca cgacgtatcg ggcgcggcgg cggagctgac
     3661 caccatgttc tgctacatcg tcggtgtgag caaatgcctg cgtgatatcc agacgcacgc
     3721 ggagtacatc aacgacaagg cagggttctt ctgacgggcg gtggcacagg gatgtgcctt
     3781 taaataattc tgtacagatt gcaggtgaat agcgtactat tacctcaagg gtaagaaagc
     3841 atttttaatc ttcccttcaa aacacgtcct gtagccaaac atgggaggac gaaatgagaa
     3901 caaatacaac aagaaaagcg atgccataca ttatcccaga agcagatttc gatagaaaac
     3961 tgaacatgtc tgagaagaac actagtcaca ccgaaagcta cttagcgaaa ggtgtggttg
     4021 atttcgttct tccgggattc acaacacctt atggttatcg ccttgtaaaa tcttgtaatg
     4081 gcgatcatta cagaatgatt actaccagcg atactccaga aacggtgtat gcggttaagt
     4141 taatctttcg ggaagatatc gtcgaagcca aaagaacatg tacgcagatc atggtatggc
     4201 gtacgcctaa tgttattcat gaccgtgctg ttcatggttt gcctcagatt ttttttcagt
     4261 tcttcctaga gcaatatgcg atcgttgtat ctgatgagca acaaacaatc gatgggagaa
     4321 gattctggga aagaatgatt tcttgggcta taaacacccc gggatataat gtatacgttt
     4381 cagatggctc tgaagaagat agacctctta gcttcatgac atcatgggat gatttctata
     4441 gccagtgggc tgatttctgt tggggcagtg ataaagatgt gcatacccac agactactgg
     4501 ttataagcaa agataaactg cattaataga agcccgcagc gcgggctttt ttgtggacga
     4561 aacaaaagtc agtgctacac tcattgacgc cacattgagg tggcttatag atggaaattt
     4621 cacaatgaaa aaagcatttg ctgcactgtt cgttttgttg tctctggtag cttcaactca
     4681 ggcctttgcc ggtcgttgtc agcacgacag cgacactgct gctgacggct cccgctgcgg
     4741 tgggcgttct gcggattccc gcccgggcgg cggtggcatt cgttaaaaac aaggccgcga
     4801 aagcggcctg tgacatgtca cgctagtttc gttttgcacg tccctgtgcc gccgcccgtc
     4861 agaagaaccc tgccttgtcg ttgatgtact ccgcgtgcgt ctggatatca cgcaggcatt
     4921 tgctcacacc gacgatgtag cagaacatgg tggtcagctc cgccgccgcg cccgatacgt
     4981 cgtgcccgtc ttcctgtaac tggttcagca gattcatcag cagtgagttc tccgtcaggc
     5041 cgagaacacc agacggcgag tgaatcaaac tgcggtagcc gggcttcagt ggggcgctgt
     5101 attctttttt gtcttccagc ttgatcgcct ccataatggc gggcatgaag cctgacaaga
     5161 ccttctccac ttttgtttct tgttgtctta aacgtttctc gcattcaatg aagtagcgtc
     5221 gtacctgacg acctttttcg ttacgctcga ccatcgccag ctctttggct gtatcaaggg
     5281 tgaggtggta ctctttgcgg ttgtggccgc ctctaccaga tgtttgcttt cccaaattgg
     5341 aaagcaaaat atagtcttga ttttcaatga attcgtattc tgatatgcga tttgtaatcc
     5401 atgctgcaaa caccttttta acacctaaaa aagcgtgcag atcgcgggca ttacaaagta
     5461 gggctgtttc gttagatata gtgccgttga atacggggat gagttgagcg ttcatgatgg
     5521 cgtctccact tagcgaatta catcaccacc gctgagacca atcagatggt ggtgaactga
     5581 acggagttgg tcttaccggc ctaagtggta ccggcgtcct ttcggacccc cattcagccc
     5641 accataattc tggcatgact gtgctatacg cataaaaaaa accacgtctg gcgtggtatg
     5701 cgccacttag taatccggga gaccaatccc ggcactggat tttgccagtg catgattact
     5761 atggcacaag aggagtgcga tgtaaattta ccgcaaaggt aatgataagc gctaagaaat
     5821 attaaaatca accatatttg gttaatgcgt ataaaagagc cagcagcgag aaattgtgcc
     5881 gcgacgtaaa ataacgagca tctgctggct tacaaccagc gatgtttcaa atttgtcact
     5941 attatcaacg tgccattcca cggcgcagag ctttatgttg tcaatcacaa cggcgaacca
     6001 tataccccaa tgaaacctat cgttgaggga atggggctag actggaaatc tcaacataag
     6061 aagatttctc aacgcttctc gaagggtatg gtggaaatca ccataccttc tgccggtggg
     6121 gtgcaagcca tgatttgtat ggctttacga aaattggcag cttggttgaa cagcatcagc
     6181 cctaacaaag tccgccctga aatccgcgaa agtattgaaa catcaaccgt atcgatatcg
     6241 acacggttga ttatgacggt aacttatttc cgtgtgtcct atttctcccg ccactaacta
     6301 attatatttg tctttaaaaa tttcatggaa tgattgagag taagacttgc catacttcaa
     6361 ataacattct tcatccaact ttcttaatga acttcgaacc tgttcgtggc atacagcaaa
     6421 aaaaacaagt cagccgccta ttaatacccc tattgatatt gggttcgcaa catcccataa
     6481 aaaatcggct aacagagcaa ataccccacc gccgccaata aatgccccta tagctcggtt
     6541 cgttttagct tgctcatagc gtctctctaa ctcactaaac tctttaatat ccatataagc
     6601 acctctataa aattacttca tcaccggatc cacctggttt attagtggcg caatccagaa
     6661 caggttattg ccgggtatca gggttcggac attatgcaca atacgatcac cggcatcacc
     6721 attcaacact cctgcggtca catcaatgat gctatccgca agaccaaatg acggtccgaa
     6781 tagagatcct acgaatccac gactggcata cctagactgt gtgccagtgc caaataaagc
     6841 ccccagccca acagcaccac cagtagcctt ttcagccatg ttgttatatt ccatcaatgg
     6901 cccaagaata ccggatctat ctataccctc aagcaccagc ttctctggtg accagtcaac
     6961 atttttccct ttcgatgctt cttttagcgc atagaccagt gagccaagag caatctgaaa
     7021 tgcagtgcca taataaaatt gcgcagttcc ttcctgtaac ccaccaagta gcgcacggtt
     7081 gtatgaagcc gttgtgaatg atttaaactg aaatatcgtt cgccccattg gagtactcgc
     7141 ccataaaggt gtgtcaccaa taccgggggt gatgatagtg ttattaacgt ctttcagaac
     7201 cgctgactgg aatactccgg caacgtactg atcgtcccat ttatcaaagt taccaatgtg
     7261 ccatccatca attacctcac catgtttctc gaactcactg cgaatacgcg cagccatatt
     7321 gtcgttgata ccgagttttg ccatgcgacg tgcagaaaac gcaccagaca aaataccgtc
     7381 tgacgtgagc attccgttca tggatttgtt tatgtcatta aatcgatcca tgagtgtcag
     7441 cttgccgaag gcatcagtaa ttcgctccat tcctgcttcg actgctgttg tcctggaaga
     7501 actgtcaaca agatcaccaa ttgcacgaga acgtgaatgt agtacagctt ccaatccaat
     7561 ccccatcttc aacatctctt ctttgctggc cttaaatgcc ggtgattggg atatctgaga
     7621 agcatagcct ttcatggtgt tacggaaacc attaaccata acccctctgg ccagatctgg
     7681 aatagctgat actgtcattc caccgagttt ggtcgtgaag ttcacatccc gcagaaaagc
     7741 gccagcacga acaaaaaacg aagacggatc atcaggcata ccatatgtac caacaagacg
     7801 atcgcgtaat gctgttatgt ctctgagatc atttgctctt gattttgaaa gtctggactg
     7861 ttctttccgt aattcctttt cgtactttcg cattaatgaa tcgagtttac cctgaggaac
     7921 aacttcacca ttgctctcat aacgtgcttt cagatttgcc acactttcgt catatttcgc
     7981 ctttattttt tcaggcactt cccgtaacag actgtcatat tcgtcctcaa ttaattgcag
     8041 acgctcagtc atagttcgtt tgccaaatgt tctcgtcaac tcaatttctg ctgccgcttc
     8101 acggatatga cgttgcagca cgtaattcac atcactttca agataatccc tgataagact
     8161 atcaggaaca tttaatgttc tttctttcgt actacctgcg gcttttacag aaaatacgct
     8221 gacaaaatcc tgtggaacct tagcaccagt aattttatta attacgatat ccgctgcaat
     8281 ttcagcatcc tcaggatcca gtgttttatt tcctctcgac caccagtcaa ccaaaatacg
     8341 tcgaaattta tcgcgttcac tgattatttt tccaacttta tatatgcgtg ggaaatagct
     8401 tgcctggcct aatgctttca gttcttcatc tggcggcaat aaaccaagct tttgcatttc
     8461 aactttcacc cgatttaata cagttcgcat cgcctgcgcc gtttcctgaa caacaggatt
     8521 agcatgcaca tcaccgcttc gcatagcatt cccaacctgc tgacgaaatg aatcaaaact
     8581 catgtcacca ccatcagctt tatactttgc gtatgcctgt ttatttccga caacaacagc
     8641 agcttcttca cgctgccatc cacgtgtacg ggtttctaca gctaccggtg tttcaatccc
     8701 cctttcattt cctttaaggg tgaaattatt ttcggctaac tccagcgttg tttttcgcac
     8761 cgtcttggac ggagactcca ttaaccttgt caaaggagta agatagctcc ctgctttcca
     8821 tgcagccttt ccaaccaccc caccagaaac aggggttaaa tcatccagag tcgctgtatc
     8881 aattttcata gcaccaacac taccaccatc ggaaagcgaa gcggcagccc tgtcagtcgc
     8941 tgatgtaatg ctcatattat caagagcatc agcaacctca cgtgtggctg cagcccggac
     9001 ggatggcgaa agcgcaacac cagcactggc aaacacgccg ctcatcatcg cacccgctgc
     9061 aacgtgagcg gcactttcac cccatgagcg tgttatttgc tgattattca gtacaacctc
     9121 gcttaatgca gtaccggcag caccaatcgc aatctgtgag ccaatacgcg ccagtgcccc
     9181 tccttgagca ccagggataa acattgacgc aacagtaacc gggtcaaaaa gcatcgctgt
     9241 tgcactggct attccaccag ccaagcctgc ttcagagata aaacgccgat cttcattttc
     9301 atcatctatc tgctgcttaa tccacgctgt ttcttctggc gagcgggaac ctgcaaattt
     9361 cgccccccag tattcatagc cgtgcaactc atttttatca gcatatgggt tataaccttc
     9421 gaccggttca aactgtctgg caggacggaa aaaaccagcc agaatattgt tctgtcgtat
     9481 tgctgcggca agcaatgaag gctcttttgg gcgaggctca gggttcttac cttctggcgg
     9541 atgcacatca aaactctgtt catcaggttc aggaatggcg agaccagcag aaataaatcc
     9601 gttattgttt gattcagata caggatagaa cggcattatt tagatcccca cgaaaagtaa
     9661 tctttaaatt tgtccatacg ttcgttatgc aggcgctgat actgctcatc cagagcacga
     9721 tgcttgtctt tgaagttttg tatagcctgt ccacgcataa tttcttcctg ctcgtgctgc
     9781 tcccgttcct gctgcatttt cttataaggt tcccaatctt ctagtgatgg cccccagcgc
     9841 atctgtcttc catgcttgtc ataaaataaa tcatccctcg taataccatc tttatctttt
     9901 gttctgatca caactgaata aagcttatct ctcggagttg ataaatcagg aacaagaatc
     9961 aactcacctc caaccagtga gcgtggcgta ttcagtccca atgcaccagc ctcagttgaa
    10021 ggtcggttaa atgaaggaga cagtttatgc aagtcatcgc catacatgat tttttctttt
    10081 tcagccttcc attgcgctgc aatccagcct gacggcccat attgataaag cgcctccggt
    10141 gcatatttca taaactgtgc ttccccgttg acctcgctga tactccaggt gcgagctatc
    10201 tgctggttgg tcatttgctt cgctacgtca gcgttaccac cagcaaggcg atagttaacg
    10261 tcatagagca tctgatagtc attgcgaaag agcgctgctt ccggcgtctg gtcatccgca
    10321 gacggatccc agcgaagcca gtgagccata ttactgacag cagaattggc tgcgctgtca
    10381 cgctcttttt tgtattctct tgtactctga acagatgaga gctgcgccct tagtgcatct
    10441 gtctggttgt atgtcttact ctgcgcttcc acaatagcag catctgaaga catccctgca
    10501 tcagttagtt gcttaacagt cagataaaaa ccctgcatat ccttcggcat atttccaata
    10561 gatgcattgt ctgtttcata taaccgacta aacaaattcg ctgcattttt aaccacttcc
    10621 tgattgctgg atctggatac tgctgaaagc tgcgtgatga cctgcgaagg catgatgcca
    10681 gtctgagcca caagccgaac aaccccatca tgagtggagg catcattaat acgaaagttc
    10741 tgcgccattt ctgtgtaatc agcagctttc tgcattgatt tgttgcttgg gtctaatttt
    10801 tcacctattg tcagcgcctc attgaatctg cgtgaatcac gttgcgcctg aattgcttca
    10861 tttgatcgct gaagcaatgc agacaatttt ccgtaagcat cgagttttaa cgcatagtga
    10921 ggatcgttaa cctcaggctt cactttctgc atttcttctt gctgctgaga aggaggtaaa
    10981 tactgaattg cctggaatat tctcgcgtta tcaatcgcta tatccagttg attgattatt
    11041 ttatctgcat tttttccata ccccctgatg atagtctcct gagccggtat ataatctgga
    11101 acctcaccgt tatatagctg ggccatggtg ttattaatag ctggctcaag ctgttctaat
    11161 attaacttcc tttgcttttc tatctgacta ttagcaaggt tatctatttg ataaatagtc
    11221 agcggatcca ttccagtttt attttttcta tatcgggaaa gccacccttg tgtttctgat
    11281 ggaagatttc ggataaattc ttcttctgat atttcacctt tacgtggatc accgactttg
    11341 gcgatcagtt tatccacgtt acccatcccc cagttatatg ctgctccggt caatatttcg
    11401 gagccgtact taccatacag ttgatttaca tagtcactgg cgagcatttc atgctgttgt
    11461 tcgtccgtag ggttgtattt aacgccacgc ttggccgcca gttctttccc tgtgcccggc
    11521 attaactggt atttcccctg ggctctctct ccagaagatg ttatcggtcc ttcaagaaga
    11581 ctaccatcag gattaaaatg tcgatcacct gattcaacaa ggcgtatggc acgcatgtcc
    11641 atgcctccag aatcattttt ctgaaacaga ccatttagcc atccttctgg attggcagcg
    11701 gcataattct tcgcccgcat ttctgtggca cggcgatcat cactttctat ttcttccaga
    11761 atgcgttctt gtgaccatcc cctggcagct ccatatctag caatggctac cattctggaa
    11821 tttctggcta aagtggcagt ttgcgggtca ttccaggcat ccgcttcatt ttgtatccat
    11881 aattttctcg ttgcctgata ttgctcatct tcataggcat ttgtctgccc tatctcatgt
    11941 ctgagaactc cagtactgaa ctgaattttc tgtgttctgg cttgttgcaa aaacatattt
    12001 cttgctgctt catcagtcaa tgaagcagct atttcttcca catcctgatc aaatccagat
    12061 atgtactctt gccccttacc aatcgcattt ttgccttgtt gtgcataaaa accggtttga
    12121 gggttataaa gacgttcatt gctgcgctga ttaagctgaa ggatggcatc ctgagacaat
    12181 gcaacattcg ctttcttcct ggcttcacca tatgccaccg catactgatc tgcgacattc
    12241 gccagcacct gacctgcttg aggaacatcg aaggtctgaa aaccaccggt ttgcacacca
    12301 cgactttgca cctggcgtcc ggatactgtt ggtacgactg gcattataat cctccgggta
    12361 atctggttcc tgctgctgcc ccgattggcg caggagtgct ttgagtaaac ggactccacg
    12421 tcccaccaaa catctggtac gcaccgtatg ccttcagagg cgcagtgagc aatgttgttg
    12481 ctgctcccac attcccctgt ttacgggctg aactggcttc tgctttatag ttggcagcct
    12541 gaacctgata accgtaagcc tcgcgttgcg cgttattcac cgtcgtcaga gaatcaagag
    12601 cgccaaactg ggcagtgtcg ccaaatatat ccagcgcgtt acctgtagat aaatcagcgc
    12661 cggtcgcccc cattgtcgcc gcctgtgtac caagccgctg tcgggtctct ctgcgccgtt
    12721 gctcagcttc agcgttacct ctgtttattg catcatttgc ctgagctgtg gctatatctg
    12781 cgttcgcttc tgcaaccttc gaggcatact ttccctgttg gtactgggtg tatgcctgaa
    12841 tgccactcat ggcgagcatt gcgccaccag caataaccgg atcgcacatt attttctctc
    12901 catgtgaaat ctgtggaaat taagaccaag agcaccataa ggcgcggctt cttcaagcct
    12961 gaatccaagc cagtgcagcc atgctttggc aacatggttt cgctcgtcga catagttttc
    13021 caggcgcgga taaactgcca gcatctgctg caatacaggg cggcagtggc gaagaaatgt
    13081 cttctgatat ttttcgatac ggctggttcc gaccagccag ggcgtaccat tgccaccgat
    13141 cattgacgcc ggagatacgc caaacatggt taccagttct ccgttcgcaa atcctgacca
    13201 ggccatagtc gcagtacgca gaccaacacg cagcgcatct tcggtagtca tcagtgatac
    13261 cgcatacagt tcgtcaatat cagcctgacg aacatccggc aaaatcatct gaagatgctc
    13321 ttcggttgcg ggaataattt gaacatcgat catcagaatc ccccaacagt aaggcgagga
    13381 ataacggcaa gaacagacag cggcaacgga tcaagctgac ggatttttac acgtccgttt
    13441 ttgccccagt tactgtccag tttcacttct acttttccgg tagcatcatc aacaggatca
    13501 tcgtagaact cgaattcacg ctgtggatat tcgtaccatt taccgccggg cgtagtcgcc
    13561 cagatgccgc gactggcatt cacaaccaga gtaacggagg ggatcacctg ttttttgtcc
    13621 agcagcgttt cctgtccgtt aatgttgata tccagtgttt cgaattcagc agttattggc
    13681 aggccgatgt gcactacagc ccccggagat tccagcgtga cggcacctcc ggaaaccact
    13741 ttctgtggtt ccacgttcgc atcagagaga atgtttacgg tctggccttc aagatgagac
    13801 aggcctccaa atgtccggcg cgccatctgc cagttcgtgg tggccacatt cctgagggat
    13861 ggcgggacgt tcctgttagc acgaaccact acagcggtat tgctggttac agaaataatg
    13921 tcgcaacgta attcttttga cacctcatcg ccagtatcag gatcagttcc ggtataaggg
    13981 aactgtagtt gcgcgccgac atcactactg gtgaagtacg caccaccaga aacactgatt
    14041 gtatattccg cgcggtaatc ccattcgcca gaaccaccag tgatggtcat cgttctgtca
    14101 gacgtatttc ttccatcata gctaaggcca gaatcaacaa agaaagcatc ttcatcgctg
    14161 gtaaataaac ggctggacag tcgctcgatg tatctcactg tttgcccgtt aacggttcgg
    14221 ttaatgacga aatacaccgc atcttcattg ccttcgctga tactgcatgt gctttcatat
    14281 tttccggtac tggactgtgg tgcccatgca aaaacccgtt gatcacgcaa ataggtcatc
    14341 accagtaatt taccgtcatc acgaatgcag aaggcgctgg agtaagggac tatcgagaag
    14401 caccagtcaa caatgctgtg cttctgaaaa agatgattgg caaggatggt caggtcgttc
    14461 ccctgatagc cgtcaacatc gaatgagtag gccagatcac ggacaacgct gcctttctcc
    14521 tggacgaaca gagcaatatt cgccacggca attggcggga cgttgcttga gccatttgat
    14581 ccttgagagc tgaatgcaaa tgatgatggg gttaacactt tgttctggtc gccggtgatg
    14641 acgtactcac ctccggaagt cagtgccacc agcgaaccga catcaatcag gtggcggatc
    14701 tcattaacct gacgcccggc ataggtgtag ataattctgt cgtcatcctg cgtaggattg
    14761 cttttgccaa aatccttata atccccagta cggctggccc agatagtctg agggaacgca
    14821 gtagatgcgg cgaagtaaag acgttgttga taataaacaa cagtgccagg ataaccatta
    14881 acactgttcc aggcatattt agcccattta tagctagcat tatcctcgcc aactacctga
    14941 gaagggatat aggaaatcac ctcggcagtt gcagtagttc catttgcagc agtgatacgg
    15001 gcaatgccaa aaccactgtg cagatattcc cactcaatgc cagtatcatc atctccggat
    15061 ccgccccagc catcccatga tgtgccttct gtatgcgaag ggcgcaaagt gcctgtttta
    15121 cctgccgtaa cagcgcgata gtagttactg tctgcacggc gaatatcgcc aatcgacgta
    15181 ctcttactgg tttcccatac cggtactgaa tccactgctg gctgttccag atagaacaat
    15241 ttgcctacct gttccgcgcc aaaaatagag gcgtttgccg ttaacgtaat tgtcccggtg
    15301 ctggcgctgg cataaaccgt cactgactcg tcaatattga tatcttcaaa tggcccgttc
    15361 ttcgttacca catcaacaag ttgccagttg tcatgcgcat agcgccgcaa ctctttcggc
    15421 gggtatgccg ggtgaaccag cgtaagcacg tcagcgcttt gcgtgaattt aattcggaac
    15481 agatcggctt cagtatatgg cgtggcaatt tcataaataa cattgctgct gttcagcacc
    15541 aacgcaccat ctttgataac gcgcatgtac tggtgtccga actccagagc ataagtctga
    15601 accgtcgaga actggaacgg gatcaggcgg catttccgat ttgagtattt ggcggcaccg
    15661 acaaaacgcg taccaggtcg attctcaacg ccgccatact gccgcacgat aaagttatcg
    15721 cacttgcgca atgccacctg gtacttcgcc atgtcgatac gaccgtacaa cgacggtcca
    15781 atctcaccac cggcaaagct gggctggatc caactgatag ccatcaggac aacctcgcaa
    15841 tggtaaactc gtcaaccggt ggctgtggtt cctgtgattc attctggcta tgcgagccag
    15901 cactaagaat cacgcgattg tacatattga gggcaaacgt accgaggtct gcattcccag
    15961 tcagcgccat gttaatagct gccgcaagac gccaggccaa cgcctccata aaaatggcat
    16021 caaacatgtt cacatctgaa acgcgagata catacttgag ccatgcctgc ggctggtctg
    16081 tgtagatcaa ctttcctgtt ccgttggtgt ctgcaccaac ttcgtactga acgcgcattg
    16141 ctgctgttgg attgcgtaca ccaggaagca taatttcagt aatgcgcaga caatcggacg
    16201 ggtactggta cgcatattcc cagtcaggcg gtggattgct cgtatctgca agcgccacgc
    16261 gtttggtagc aaagttccag tcaaaatcag aaagaacagc atcacggcag gcctcaaagt
    16321 gcagcgaaca ttcccccgct tccttgctgg cttccgtcag gctgttaatg ctgcgactgt
    16381 tgccaatatt ggacagcgca cgattacaga tctctactac agaggccatc actcacctcc
    16441 gttaccgtac agagtttcag ccgctgattt ttctacaccc ccggaaacag gagcgatcgc
    16501 catatcagtg atctgcagat cggcgctgcg attaacacca tcgtcagttt ctctggcaga
    16561 caggcctcga ataacagcct ttgcagttat catcacttcc gttccgacgc ccttaggttg
    16621 cgccttcagc ttattcaatg tgtcgttatt aagagtgatg cacagccccc atgggtattc
    16681 atcgcgagtt ctggtttctc cgctctcatc ctggtagctg tcagtgccgg ttttgaggtt
    16741 tacgagttcc atatacactc ctgcaataaa ggggccgaag ccccttgtct gattcgcgag
    16801 gcttacacgc ccagttcttt acgcttatct gcgatcttct cgcggagcgt ttcagctttg
    16861 gcgttatggt gtggcttctc gttaaagagc aattcgtact cttcacggag cttatccagt
    16921 tcaccatcat ctgacacatc gttgatgatt ttggtgctgg ttgctgccat tgacaccttt
    16981 cctgcaactt ttgcttttgc ctgtctggct gcatcgttaa caggttccag tgcgctacca
    17041 ggctcacctt cgtattcgat ttctgccccc tccggccaca gagtgttatg gatatgagag
    17101 aggcgcagaa cgcggtatct tggtttctca cctgacatcg atatcacctt aaccagttac
    17161 ttttgagcgg atcggatacg gcgtattggc atcaacatca agactgatac cagcagtgaa
    17221 ttcgccagcc gttagtgggc cagttgcgac ggagtagtta acacgcagat atcgctgaac
    17281 accggcaggc acctttgcag aaacaactcg tttacctgct gtcagggcgg tctttgccag
    17341 tgcgccacta tcataaatag tggtccatga gctgttattc tcactcgtct gcaactggat
    17401 gtttacagtt gcatcaccgc ttgccgcggc ggctgtgtta accagcgccc aaaactcaag
    17461 cgggtaaccc acgccgatat cacgacgttt tccgtcaatt ggaccgagat cgattacgtc
    17521 agtagaagcc gcggtattcg taaccgcctg agcttcggag aacatcaaca gtttgtcggt
    17581 gatcatcttc tttctccatt agtgggtctg ttacgaccca caggttaata acaggcgtta
    17641 caccacacgg gcttctgttt ccagaagcgc atcagtctca cggattggta caccacggaa
    17701 tgaagtccac cactcgcctt ctgtctcttt tacgctgatc gccagagatg ttttctccag
    17761 agattgcaga tcaagagcct ggcctacagt gcggttcatg tagaacaccg ggcggcccat
    17821 gccacggttt ggaatgcgat gcagtgcttt aaccatcaac ttcgcaatat ttgctgcaga
    17881 ggaaggttct gaaagattgc tgacatcgat gtttgcaatg cgaacaacat aacgccagtc
    17941 acgcagagca agcccgttgt cccatttgta atgggtacgg tagccttcgt acttgccgcc
    18001 attcgcatct tccagtgtca cctggccttt atcttccatc tggatgccag ccttctgccc
    18061 tttcgggaag atgccatgca cggtgttttc gccccacacc actaaccaga tagaggtgtt
    18121 atctgtaccc gtgccaccag catcaatgat gttctgagca ttacccgcag acaggctgga
    18181 atagcgggag gacagtccca taaactgctg agggttaacg ctggaatcac cataaaacag
    18241 cgtctgcgcc atctgctgat tcatcgcttc aataaatgcg cggtcttcag acaggcggaa
    18301 ttcggcggta ttgccgttca gatcagccag tgacttatcg acttcagcat aggtttccag
    18361 catgccaacg gaatcggtta cctgcactgt ggttgatttg cttggctgta cgccatagtt
    18421 cagcaaacgc caggtagctg aaggtaaacc agaacgaatg gtggttcggt gtccggtagg
    18481 aaggttccct tcgacaaaag gcatatcctg aaggatcggg ttagtttgac cgagaagctc
    18541 gataatctta tcgactttcc cgtttggatc gacgcgctta ccccagtcag ccagcgttag
    18601 cgcagttaag cctttaacag ccattgtcat ttcctctctt atttgccata gagcacttcg
    18661 gccgcactac gctggccttc attaccaccg gtgaccatgc catcttcaga catcgccttt
    18721 ccgattttca cgaacgtctt gaccagatca gggtgattac ccagtccggt ggtgttcaga
    18781 tattctttga gctcaggtgt cccgaactgg tcaagcgcac gctgcgcggc gctaaggtta
    18841 gaaatcaact tgtcgccacc gatttctttg tcagctttta catcagcagc ccactgctcg
    18901 gttgttttct gccaggcttc tgcctggcgc tgctgaacac ctgccagaat cttcggataa
    18961 gcatcaacca gcttttgcgc ttgctcgttg gtcaggttta gttctcgcgc caccggctcg
    19021 aattccttca acgcttctgt atccagctct acgccttcgg cagcctgaaa ctcgtacttc
    19081 tcaggcgcac cctctggttt atcgccgtcc tttttttcat ccagcttatc gttttcaggc
    19141 tttttgtcag cagcaggttt atcgccatca gcaacaggtt gtggcttatc accttcctgt
    19201 tgtgatggat caccaactgg agcagggtta tcacctgcag gcgctgacgg ttctgacgca
    19261 gccggagctg ctccaccatc gactggttgc tcattgcaaa gacggctata cagcaaacgc
    19321 tcaaataaat tcatgatcac tcctgttcac tggcctcttt ggccatcttc aaatactgtt
    19381 cagggcaatg cgccataacg cgctgaaaca gttccagcgc cagattgcgt tgcccctcat
    19441 taaatgccat tgccatagcg tccatcggcg agatagcgga aaacacacgg cctttctcca
    19501 gtaccgacca gacaacccga cgcccctgtt cacagctcat gacaaagcga atgtcatcaa
    19561 tttcacgctg tgccatgtca cgttgcttac gggcgttttc ttctttcagt tgatcgtctt
    19621 cgtaatctgt cattgtgatt gcccaccctg accactaact gcattcgcca tagctgacaa
    19681 aacactcgga tccgaagttt tagcttcgct tagcgtcttg gccccctgtg ccgccgccat
    19741 ccccatcgcc atcatttgtt gctgctgttg ctgctgtgcc cgttgctggc gagcctgctc
    19801 aacctgttcc tgcggaacaa tgacggttgg agacactccg gacatatcag cgaatgcatc
    19861 gatcgcctga tcaacgttga gtttgtcgag agcttctggt ttcgcttgcg caagttgacc
    19921 aatgaagtta accgtggacg ccagactgga caggccgata gacttctgcg cctgagccat
    19981 gacggaaatg tattcgacct tcaggggcat gccttccatc gcgtcaggcg gtggcggcag
    20041 catgttttta cgcaccatca tcgagaaagc gcggtcaatg agaggattaa gacattcgtc
    20101 gttcagacgc tccagaaccg gccccaacat cagaagtttt tcttctttca tttcgatcac
    20161 cgcttcaaca ggcatcgagc gggtattgat gttctgcaac atcatgaaca gatcgacaaa
    20221 gtaggcgctg ttaatgattt gacgagtgtc ctgaatgtct gccaccaaat ctgctgtact
    20281 ggggttaacc agataagcag gcctgaagcc atcctgacca gtaatctgat cgatatacgt
    20341 gatgtcgcca ggaagaaggg aggcgcgctg attcttgagg gaagtcggag caaccatcgg
    20401 cggattggtg gctttatcaa tcaactgcga cttgcgcttc tggagaagct gcaatgcctt
    20461 aacaggtcca agcgccagca tgcccgggca tgatgatcca taaacatctt caccgttaac
    20521 ttcccagcgc ggagccataa ttggaaactc atcgaatccg gactcacgca acaacttgtc
    20581 gttatcgcca ccaacctcgt aataaaccga tttgaatggc ttgttcttgc tatccagctt
    20641 cgatgtatcg cggtcaatgt tcgggtaaac cgaatgcatc acgtcaatcc acttctcgta
    20701 ggtgccgctt tcccacatgc tttttacgga ttcgctgacg ttatttagcc cgaactcctg
    20761 aacaagctga cgaacagtca tagagaactt gcgaaaacag gtgtcaacac tgccacgagg
    20821 tgagttagcc aggtagtaac tgcctatcgg gaatggcatt gtgcgaatga tgtcctcatc
    20881 atcctccagt accgccattg caccggtgct gtatgtgccg aggcttccgt ataactgcgg
    20941 cagcgactga tagagattcg acttattgaa catatcgttc atgcggttct gcaccgcctc
    21001 aagccacaac ttaacagggc cataatccat catttcagga tctggcgtag ccaggcgaaa
    21061 ccacggacgc gcagggcttg tgatgcctga catcatgccg ctggcgagag tgcgcgccgc
    21121 catagtcccg gtcgaatcaa taatgcgtgt attgcgccga tcgttacggt tggcctcaga
    21181 agtcagaaag cgggaaccac gcgggttgat gtaatcactc aactcgcgcc agtgcggctc
    21241 gaacgactga cgctcgcttt caagttgtgc gaactgtttg ttcaatcgct ctttagttgt
    21301 ttccgccatt tcaatgactc cggttactga ccaagcagcg ttttaccgct ggtattagcg
    21361 gttgatgtgt cgccctgaga accggtaaga agcgtagaac tacgaccagc agcagcgcga
    21421 cggcgacgtg tttcttcgtc gcgggcatca acaacggcgg catcctgctc ctgtggtgct
    21481 gcctgaactt ctggtgttgc aggcactgat ggtgagctac ccatgcacat atcaatgact
    21541 ccgtacgcaa ttaaattatt accaatttaa ccacaaatga tttacctatc gtagatagtt
    21601 gacatttaac gcacgaatta ttacctttca ggtaagcaaa gagttcattc cggttattaa
    21661 cctgactagc ttgtcgttaa attgaacagg tggggtgaga ttttattttg agcagtacgg
    21721 cgtatggcac atgcgccgat agcggtctgg atacgtttaa ggggcactct cccttgctct
    21781 ggcaaacgaa ccaggtagcc gggatgtgca agtcgagcgg ttttattccg cgcacgggga
    21841 ttcaccatcc cggcgattcg gtgtgacacc tcggaagaga cgagggtaca acgatgagag
    21901 catttatgga accgcgacaa agtgtggcgc cttaacaggc taagtgctct cagcgttgtg
    21961 gcattagctc agttggacag agcaaccgcc ttctaagcgg ctggtcgcag gttcgaatcc
    22021 tgcatgccac gccagaatca cgcctaagga ccgtgatgcc agaagttcca ggtgcttggc
    22081 ggtgatggtt tccattgaag gactatcacc gcccttttta cagcaggacg ccattgcgat
    22141 gacttcatgc tgtaaacccg tacagccacg gaaggcataa ctcattgctt acagttcgcc
    22201 cggttcaccg ggcatttttt taaggtgtga atcatgaaat acgaattcga tggattttaa
    22261 tgtcgtgaca cgtcacaaac agccagccga taagctggct ttgttttatc ctcatcagag
    22321 gatatcaacg acattatccc ctcataggga tacgtttttc ataatgaccg aagattacct
    22381 taaaggtaga atcagaaaaa aacctaaaag ggcctggaca tgcttgaatc acttaaagaa
    22441 tacctatctt caaccgttaa taccgcttcg caaagggtca gaaatccagt gtttggagcg
    22501 ttcgcactgt catggtgcgc ctttaactgg aagtcgattt tatacttact cctcagcgac
    22561 tccagcatca tcgacaagat cacctacatt tccagcaata gcagttggaa aacggtatta
    22621 gcattcccat gtgtttcagc aataatcctg tgcggcggca tgccctggat caacaacgtg
    22681 atttcaaagt ggcaagccaa acctcttgat aattacgatt cgatagagaa ccaccggaag
    22741 gccaagcgca ttcatcgcgc cacacgcctg cagcgcctga aggcaaagca cgatgtgaca
    22801 tacgacaaag ttaagactgg cgccgaaaaa gacatacagg ctatgaagga aagaattacg
    22861 gagtcacagg ccaggatggg tgagctaacg tcggaaagag ataggctctc cgataaggtt
    22921 ttggaattga ataagaagct ggaaaacatg caatcagtgg ctgcagcgtt aaagattgag
    22981 gcagaaaagt ccgccagaat gcttgaagat agggaggcca aaataaataa tctcatacaa
    23041 gaattaaagg aaaaagaaaa ggaaagtatc gtattgcata tgggccatga acccttgcga
    23101 aaaacatata catcacaggc tgagagaatg attgctgaag ccgtgaaaaa taagcaataa
    23161 atcacgcaaa cgggtcgtaa tctgtgatgg ccttgccttg ctggttctgc tgaccgggaa
    23221 ttcgcaggcg cttcgacaca gggaaagcaa acgtcagcaa tagcgcatcg cctttccccg
    23281 gcgaacgccc aagtcgttct ttgatatctt ccttcggttc gataacgatt ttaccgtcca
    23341 cgcgaacttt gtactctgcc gtcgacaggt cgtccgctgt ttcctggtca tccagcatac
    23401 cgcccagcct cagccatgtc ttacatgagt tgaacatctc cccacgcttg ttgagcatct
    23461 gcgggtcagt agacgcgcca ccgaacggaa caagttgcca tgtacgtccc cagccatcac
    23521 cgattgactt caaaccggtt ccgtaaccga agtcgatgaa taccgcgtca gcctgatact
    23581 tgtcttcaaa gtcagcgatg cgcttcgcca taatcagatc gtcagtggtc ttgttgccag
    23641 tccacagcac cttactgtgc agcccctgcc gcaggtatat cacagcgtca tcaacgccgg
    23701 agtatgccgg gtcaacgccg attatcaccg gagcatgtgc cacctgcgca gcagttacca
    23761 cccgtttcat tgcctcgtca gtaaggccgg tagggataaa ctgcaattca gatgcatcag
    23821 ggaatatgcc gcgcacacgg attttaacga agtcgctgtc ttccccgtag tcatcaaccc
    23881 atttctgcaa ctgctgtttg ttagtgcctt ccaccgtccg gctgtcaatc tgcgcagttt
    23941 tccagcggtg tttatatttg cggaaacatt cgcggaaacg tccggtatta cgcgtcgggt
    24001 ttccgaacgc cacccagata atctcagtgt cttcgtccgt aagcgccccc tcggcaactt
    24061 cccacaccag atccgcaatg ttcgacgctt catcaaacac cacgatgatg cgtttgcgct
    24121 cgttgtgtag tccggcgaat gcctcggtgt tgtgctcaga ccaggggatt gcgtcagctc
    24181 gccaccgctt gtcgtgccca ggatcattgc tgtacatcgc ggtagcggta caggtaaacc
    24241 agtctttcgt gatagcaagg ttcgaccact tgataatttc cggccaggtc ttcgttcgta
    24301 gctggttgtc ggtgttggcg gtcaccacga ccttacaatc ctcgcaagtg gacatgcccc
    24361 agttgatcag cattgagatg aatgcggatt taccaatacc gtgaccagaa gcgcgtgcca
    24421 gcataagcgg ctgatagcgc gtctctggat tctgcaggtg atcacgtatc tctcggaacg
    24481 catcagcctg ccactgacgt gggccggtag catgtgccag ttcagtcccc tcttcccccc
    24541 aagggaacgc atagagggca tagccaagcg gatcgtgagt gaaccctgca atatcctcga
    24601 ttaactgctc ttcaggagat aacgctgtat ctgtcactga ttgccatcct gacgttcttt
    24661 gagtcgcttc ctggctgctg ctatgcgatc agcaattgtc acattcacat taacatccaa
    24721 gcgttctttg aatgcgttga cgtcgacgtg cttaccaatc agttcgaggt tcttcacctt
    24781 gtcaggccat ttaatttttt tgaggattgt ctctatcgaa tcctcgttca tgttcatgat
    24841 ggtcgatgac agatcaaagc cgctaagcgt agtgcgccag attttcggcc actcacggat
    24901 tggtttaagg ctcccatcgt cgttgaggat gtcgatcacg tccatctggt cgatctccac
    24961 taggcgcatg agaacgtaat cagcactgac gcgcattcgt ttgttgcgct cctccatcaa
    25021 ctcggcaatc cgtttttgaa tgcgttcatc gcgcatcatg acactggctt taactgccgc
    25081 tgtatttggg gagaatcctg cgttaatcgc tgcctgagtc tggttttcag gcgttttgat
    25141 gtatgactgg caataagcct cctgcattgc tgtgagcggc ttaaattgcg ttgatttgcg
    25201 tttataggtt ttaggttcag caggcatcat aaccaccgtg gtaatagtta ccgttgtggt
    25261 aatagtaaca tgcaaaataa agccgccata gttggcggca gtattcaaag tccatcaaat
    25321 tcatcgtaaa aactctcgtc aagataccct tcccatttac cgcgaatgaa aattacatcc
    25381 tcgccgcaag ggtgctgact gtcgataact atatccctcc tggcgcaacc atacttatgc
    25441 atgagaaatt taacctcttt cggaaaattt gctgagttat ctctcatatc ttcaaggtcg
    25501 tagcgtattt ttggcataac acctcgtgac atgtcacact attaatttcg tttcatgcca
    25561 gcctttggtc acccagcatt gcgagtcacc attacacggg catgaattca caggaactct
    25621 ctcgccgcac ttaccgcaac gttttctgct gatcgatttt atacgcccgc gcacgcgtgc
    25681 atcatcctgg cggatcagta acgctatata ctcaccaaat tcgtaaggcg cacgcccggg
    25741 gcgacgtgtg gcacagttac gctccagcat ttcaatttcc tgagcatcaa gcacaatctc
    25801 cagcttacgt acaccggatt cagcttgtct ggctctctga gcggctttgc gctctgctgc
    25861 tgatttagcc atcaatattc acctttatcg cgaacacctt taccggttta tcgccgaagt
    25921 ggggatgtgt gattgtcttg atttcatatc cgtcatacgg aatatcaatt ctgcggctgg
    25981 aatcgtcgcg cttcggatat ccctttgtga taatcaggcg gtcatactcc cggaacataa
    26041 ttcgcttatt ccaatagtca ttacacaggc gatactcctc ggttttctcg ccacgaatca
    26101 tggcatcgaa gtattcacct tttacggcaa gttgcaggtt agccacgacc ttcctccttt
    26161 ggcttgtgaa tttgtatcgt catgccgctt tgagtggtga ctacaatgac agaaccaggc
    26221 tgaaggctgt taagattgaa tgcttcgtaa aacgaatcca atgccagtgc ttttttattc
    26281 tttcggttcc accaacgcca tcccttgcta caggctacac tgacaatcca ctgcccactc
    26341 ctgtaagcca tataaaacca gatgagcaaa acctgaagga aggccatcca gtcaataatc
    26401 gtatatttcg cgaaggagtc catcaattca cctcctgcgg cggttctggt agcggcatcc
    26461 agaacaagac gttccctaac cacgataaag tgccgtcgct caactccacg tattcccctt
    26521 gtacctgtcc tgccatatac tcgccgtgct ttgaataaat taaaatccaa tcatcttgag
    26581 cgggcattcg ctcactacag cttatccaac catccggagt taccggagag ttgcccgata
    26641 gctcgttcaa cttgtaagtc tggcttacaa gttcggcttc caattctgct atgcgcttct
    26701 ctgatgcttc cagctcaaca cgcagcttcc ctaccgttag cgcaatatcc tcgttctcct
    26761 ggtcgcggcg tttgatgtat tgctggtttc tttcccgttc atccagtagt gccagcacgg
    26821 tttctggtcc ggccagaagt ttgaaggcgt tgagcgcatc aatatccaca ccgtaatctt
    26881 taagttcctg ttcacttaac aaatcatcat caactggcaa cattaacagg cgttccattg
    26941 ccggaattgc acgttctgcc gccattcgca gtgcctgata gtcaatcttg ctcactggtt
    27001 gcctcctttt cgaagctctg cggcaaagtc agtggcatgt tcaccgagta gtttccagtt
    27061 atcgcgtata tcagatgcaa atgactgttc ggattttgag ttacatttct gtgcaaacat
    27121 ctccaatccc tgcgcccgca cttcagccag gcatttgcga aactcggaaa cgtactgttc
    27181 gacgctcatt ccccagctaa gtggacattc attgaatgtt tcgccttcgt gctcttcatc
    27241 aggtagctct ttggtaaaga actcacgctc aatggcgtgg agtgtgtcag caaaacgacg
    27301 taagttactc aaacctaccg taatggagaa ttcaggagca tcacatccga cgcccatctg
    27361 ctgataaacg gcggttttga aggccttcag cgccgcattc tccgctgcca gctccgaaaa
    27421 cttctcgtgt gccaacttaa cagctgcatc agcctgctta attgactcaa tcgctttctg
    27481 ctggtcttcg gccagcgcat tagcacgcac cagttgcact tccagttgcg ttgccaaatc
    27541 gctgatcagc tttgccacgc tgcgcatatc aacggcacca cattctgctt tcagttccga
    27601 agccatctca tgcccggcgg taactaaccc tttgatatta cagtccatct ttaccctcgc
    27661 ttatccacat aacttattga ttacattgat aactaaaaag atcgtcgatt catgccgatc
    27721 tatttttcat cagtgattca taaaactttt gccacttatg ccgaccgaaa ttagccttcg
    27781 agttacagct cgaacaaagg caaataaggt tctcctgacg acagtcttgc ttgtcgtaat
    27841 tgatgtgatg agttgttagc cttttgtctg ttccatcaca acctgggttc tgacaagtaa
    27901 aaccatctcg ctcaataacc ctcttgctcg tttctcggaa atcccacgga tacggcaatc
    27961 tcgataatcc accagaccag tttggatttc cttccccctt catcagagaa gaacgcatta
    28021 tgttggaaca ctgtttcgag cagcatttat gacgcttctc atgactcaag aaaacggaga
    28081 acgaagatcc gcatacgcag catattttta atactcgctt tagttcccga tttaccgtct
    28141 tgcctcgctg tgaaattcct acacactgaa gtgagcagaa ccttgaagat gagttggctc
    28201 gcttaacctg atattgcctg ccacatacgg ggcaaacctt ttcgattttc ccacccttcc
    28261 aattcgggtt tttatcaccg gatacaatca atcccgcagc attgggcttc tcgctcatag
    28321 ttcctcaacc tcccatccac caccgagctt tttaggtttt ggataagcaa cctgaaagac
    28381 aaacggatag ctgtctgccg caaccttcat cttgactctc gcatcgtcag taaagactga
    28441 cttacttccc ttaacgtcca ccatcaccaa ctgcccgtca gccaacatca cggcaaaatc
    28501 tacagtcagg aagcagttgt cagccagccg cagcttgata ccctcgaacc gataccaggc
    28561 gacttcccct gcacgtttac gctgctcaag gtgctggcaa tacgcagatt ctgttttgtt
    28621 catctggcct gttttgagtc gaccaagagc ctgtatctgt tttctcatga tttacctcta
    28681 aggtaattaa aaaccacata aggcacaaaa tcaatagatc taagaatatt ttgttaccca
    28741 ttgggtaatt attgaggcgt aaaaaaatgc gctatcgcgc tggtattact tgataaatcc
    28801 tgctgccttt cctcgcctgt attcctccat cagccactgc gccggtgtta ttccccccag
    28861 ggtggcggcg ttaggcatgc acccgaaact tcgccctggt ggatggtaaa cgtctctccc
    28921 tgtgtccgga ggtgtactca tgggttctgg ctttgcctgt atgctgatca ccggatcggg
    28981 tatctgctgt ccggaagcca cctttttcgc ccaatcatcg agcaacctgc gcgcgtgttt
    29041 ctcaacctca atctcgctaa gctggcgctg atacattgca cggcgggtat cacatacgac
    29101 ccagtacata accggatgtc gccacgggaa tctttcggga ccaccaggat ataaactttt
    29161 ttccttgctg taccggtgaa actccgccat cacatcgtca atggtgacgc caagaaccat
    29221 cttgctgtct ttacaccact tgatgaattg ccctggcgac ggccagaacg gagattcact
    29281 ggcgcgggcg tggcgcatac cagcagaaac ctgttcacgg gttcggatcc ccccttcggc
    29341 aaacgcagca atccactgct gttttgcagc aacttcctgc tctggcgtct tcaggttggt
    29401 taccactgcc gccggaaaca gttgtttcag ctgtttgaaa agggcatcaa caagcctctc
    29461 tgctgacatg ttcaccacgt tgtcattgtt ggtgtactga tgctcataac ctgacatgcg
    29521 agaaagggct tctccgtcac ggttttgtat cgcggtaaaa acgttgttca caagaaatcc
    29581 tcccatgctt cagggctgtt ccagtgcgga acgttgttat caggtaatgt tgattgcttc
    29641 tgtctgctaa tctgcagccg ccttgccagc ttctgctccc actgtgcctg atggtatgcc
    29701 ttaccctcag ccatccagta aattctgaac tctgcaagtt cctgtgccgt tggcagactg
    29761 tccaggtaga tcccctgcaa tgagcttttc cgaagaaagt catctgatgg ttgccattgt
    29821 tcatgcatga caaatttgcc taattgccct ggcccaccag gaggaacaaa gttattcatc
    29881 acggcgttgt ttgcgccggg gtcatgaggc acagaatccc cggtttttgt tctgctctcc
    29941 ctctcttggt taaatgactg gttatatgac tggttctgga tcccgttttt gggatcattc
    30001 aacatcccgt ttttgggatc attcaacatc ccgtttttgg gtatattccc gttttcggta
    30061 acattaccgt tttcgggttc attaccccct tcccggttgc ctttaatgtt cccgtttttg
    30121 gttatattaa gagagaaaac ccgcactctt ttcgtcgctc cctttctctc tccggtatct
    30181 gaaataagcc ccattttcat gagcgatata agcccggact gcacggtttt tttattcagg
    30241 caagtgtctt taacgaggcg ttctatgctg gggtagcaga ggttatattc atcggctctg
    30301 tcagccatcg agagcagtat gagctttaat gatgagctac ctgggtctgt ctcccaggcc
    30361 caatctgttg catgtctgct catgattaat ctccgctatc agcttgaatg ttgtggggag
    30421 gaattaatca tgatctgctt aatctctgcc ctgatgcgac ggtttgattc catggtgcac
    30481 tcaacacagt gtccgttgta aacccagcgt tcactgtcat gtccgtgctt acatgatttt
    30541 ccggtgtagt agcgtttaag tccgcgcttt gcggcatcaa tacgtgtaat gatttccatg
    30601 gtaagccctg ttattagtat tgggattacg gttattttgt gctgacacaa aaaaaagatc
    30661 aaccagattt ggttttttat tacctttaag gtgcgaatag atatgaaaag accgcaggat
    30721 ggcggtctac agaggtttgt ggctggatat catgagtaga agaagtatgc cagttctgct
    30781 tttgagcgca gccattgtct tgttttacag gctttaaaaa gcccattcat caatacttta
    30841 cctggcattt tgcgcttacc tgttaagtga gtctggatat agtgactcgt cgttccggct
    30901 tcctgtgcga aggcttcacg ctcatccgga gtaagtgcaa gccagtgctt tttgaaatcg
    30961 aaatgtccgt tatcgctcat agctattgcc tgatatttat ttcagataat aaatattcac
    31021 ccgtaaggta acaaatatca aggatagtta cctatggggt gcatttacct gttgggtaat
    31081 attgctttaa attgaatcat ctactgattc atatatgagg cgattctcca gaaaatgaaa
    31141 agtatccagg acgtccgcag gcaaaatctc aacgacttga tcgaccgtga attcaatggt
    31201 gttcagacgc ggatggcaga aaaacttgga actcaggcaa atctggtaaa ccgctgggct
    31261 cttggcaaga aggttatcgg cgaccaggtt gcgcgaaaaa ttgaagctgc cgccaataaa
    31321 ccccgtaact ggcttgatat cgatcgctcg ctttctcagg aggggtttca gcctgtcggc
    31381 ccaagcgaca ttggtcagct ggcggctcac aacctggaac gctggatgag cgaaagccgc
    31441 gacctttcaa cacagggaaa acttcaccgc gcatccggcg tcgcccaggt gacaatcagc
    31501 cgcctgttaa acaatgaggt cagtgtttcc atttccaccc tggagaatgt tgcatccgca
    31561 ttcgggcgtc acggatatga actactgatt cacccgcacg accctgcgac tatcaactat
    31621 gaccgctcgc gctacgcatt gttacccgaa accgagaaag caaagatcga aagttacatt
    31681 gaatttgtca tcaaccagaa cgaaaaaaac aaacaataaa atcatatttt tcagtaagta
    31741 agccgccttc tggcggcttt tttattgcct gttcaattac ctaatgggta atttttttaa
    31801 ctcatatcta ttgacgtcaa accagatacg cataattatt acctaaacgg taacagaccg
    31861 aggtaacaag ttatgcagtg gaaaatcatc aacggttggt actgcgttac tgcatgcgga
    31921 ttcatgagct ggaagttccg caccttacag gaaggcatta agtgggcttt cgtcagcaaa
    31981 gaagctcgcg atgtggccaa cgataacgag atatgggagg gatgataatg aacgttaatc
    32041 agcagaaaaa tcttcaaaaa atcatgctgg cattcgacaa ggactaccgc ctgtcagaac
    32101 agctatatga ccgacaagtt gaactgattg agagtatccg gcttcaccaa ctggcctcaa
    32161 ctttcgacgt tgtaacaggc aaaggcgttc gtcaggaagt gctggaggct gctaaagaca
    32221 gccctgagtt cgaagaactg atggatgcct accggcgcga ggcaatggca attatcgccc
    32281 gctgggatct ggcggatcgg attgatgggc agagggaagc ggcatgatgc agaacgctgg
    32341 aatcatggat agaacaaaat acatcggagg aagcgatgtt gcagggattc ttggaattag
    32401 cccatggcgc accccgcttg aggtttatct ggataaggtc aagccacgtg tcaaaccagt
    32461 agacccaagc aagcagaaag ttttcacgcg tggccagcgt atggagccat acgtaataga
    32521 cctgctttct gaggaaacag ggatggaaat cgttcatcgc ggaaaccgct atatccaccg
    32581 tgattacgat tttattgcag ctgagatcga tgcagaagca gcgtcaggcg agaacattga
    32641 gatcaaaaca gttagtccgt tcaaagccaa agaatgggga gaaatccaga cagatgcaat
    32701 tcctgtgcat tacacggccc aggccatgca cgggttgatg gttacaaaca aacaggcatg
    32761 cgttttcggt gtgcttatcg gtggtgacga cttccgaatc tatcgggttg agcgtgatga
    32821 agaaactatc caggcgatct tagaaaaaga aatcgctttc tgggaccgag tgaaaaatct
    32881 taacccgccg gaagctacca gcgtaagcga tgtatcgctg atgtttgaga aagatgccgg
    32941 gacaagtatc gaggctgacg gaaaggcact cgcactattc aacgatctac gagacatgaa
    33001 gtcacgcaga aaatcactgg aagaagaaat agctatatca gaagagaagc tgaagatgta
    33061 catgcaagag cactcagtcc tgaccctgga cggaaagccg ctctgcacat ggaaatctca
    33121 gatcagcaac agattcgacc agaagctatt ccagtcagta caccctgagt tattcgaaaa
    33181 attcaaaaca acaacgacac aacgcgtctt cagaatgaag taaggagaaa aaatgtctat
    33241 caatgcactt aaggcagcgg ctaccggtaa ccaagttgca catcataatg agaaaccaac
    33301 aactctggcc ggacttctgg cagacccaaa aattaaagct cagatggctt tggcacttcc
    33361 aaagcacatg acagcagacc gtctggcgcg catagcaacc acagagatcc gaaaggttcc
    33421 aaaacttgca tcatgcgacc aagccagctt cctgggggca attatgcaat gtgcccaatt
    33481 gggtcttgaa ccaggcggag ctcttggaca cgcttacctg ataccgttcg acaaacgcca
    33541 gaaagtaaat ggaagatggg aaaccgtatc tacagaagca cagctgatta tcggctatcg
    33601 cggaatga
//
LOCUS       DAZFYC010000042        33427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFYC010000042 DAZFYC010000000
VERSION     DAZFYC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.042"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(180..275)
                     /gene="dqlB"
                     /locus_tag="ACHKW5_002258"
     CDS             complement(180..275)
                     /gene="dqlB"
                     /locus_tag="ACHKW5_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO7511648.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
     gene            820..2100
                     /gene="trbA"
                     /locus_tag="ACHKW5_002259"
     CDS             820..2100
                     /gene="trbA"
                     /locus_tag="ACHKW5_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO7511649.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FSFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFIAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDEVI
                     PRRHDNDEGSETGETTETTETTETRHPADKEKAQ"
     gene            2097..3215
                     /locus_tag="ACHKW5_002260"
     CDS             2097..3215
                     /locus_tag="ACHKW5_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO7511650.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLNAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSAAPAITADKNTLVTEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNQTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            3196..5496
                     /gene="trbC"
                     /locus_tag="ACHKW5_002261"
     CDS             3196..5496
                     /gene="trbC"
                     /locus_tag="ACHKW5_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO7511651.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLLQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHTYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMILARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMMAQDQGRIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAE
                     NNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(5717..8437)
                     /locus_tag="ACHKW5_002262"
     CDS             complement(5717..8437)
                     /locus_tag="ACHKW5_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO7511652.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMTAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWHSVPKDIFTQVIPGERFRG
                     HSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYALIQCRSQIEEMIAGGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPKCRYNPELAASDEVEPGFRRDPELRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAHIRVQFRDPQVRKLHYHIAEVQRMQALIRLK
                     ENVREERLALMAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNADRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM"
     gene            complement(8449..8781)
                     /gene="mobA"
                     /locus_tag="ACHKW5_002263"
     CDS             complement(8449..8781)
                     /gene="mobA"
                     /locus_tag="ACHKW5_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO7511653.1"
                     /translation="MSEKKTRRGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            9014..9349
                     /locus_tag="ACHKW5_002264"
     CDS             9014..9349
                     /locus_tag="ACHKW5_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO7511654.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIILEAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(9434..10282)
                     /locus_tag="ACHKW5_002265"
     CDS             complement(9434..10282)
                     /locus_tag="ACHKW5_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7511655.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDLLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEHGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            10630..10827
                     /locus_tag="ACHKW5_002266"
     CDS             10630..10827
                     /locus_tag="ACHKW5_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511656.1"
                     /translation="MQGAQVTARLKKNDGTTMACRGHWRTTPAQVKTEKTTRHIQSKK
                     SEISSVFHSFYRANLVTMMIK"
     gene            10861..11112
                     /locus_tag="ACHKW5_002267"
     CDS             10861..11112
                     /locus_tag="ACHKW5_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7511657.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(11143..11349)
                     /locus_tag="ACHKW5_002268"
     CDS             complement(11143..11349)
                     /locus_tag="ACHKW5_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511658.1"
                     /translation="MTLAERLIQKGFDEGFKEGFKEGFKKGALEVAREAACRLRDMGW
                     TPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(11411..12301)
                     /locus_tag="ACHKW5_002269"
     CDS             complement(11411..12301)
                     /locus_tag="ACHKW5_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7511659.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKKIIH"
     gene            complement(12298..12687)
                     /locus_tag="ACHKW5_002270"
     CDS             complement(12298..12687)
                     /locus_tag="ACHKW5_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO7511660.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRSGLVY
                     ELYQRRLSAAIDNFVSTLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(12878..13144)
                     /locus_tag="ACHKW5_002271"
     CDS             complement(12878..13144)
                     /locus_tag="ACHKW5_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511661.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(13237..13671)
                     /locus_tag="ACHKW5_002272"
     CDS             complement(13237..13671)
                     /locus_tag="ACHKW5_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO7511662.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            14022..14092
                     /locus_tag="ACHKW5_002273"
                     /pseudo
     CDS             14022..14092
                     /locus_tag="ACHKW5_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            complement(14358..14858)
                     /locus_tag="ACHKW5_002274"
     CDS             complement(14358..14858)
                     /locus_tag="ACHKW5_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO7511663.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(15327..15923)
                     /locus_tag="ACHKW5_002275"
     CDS             complement(15327..15923)
                     /locus_tag="ACHKW5_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511664.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            complement(15920..16639)
                     /locus_tag="ACHKW5_002276"
     CDS             complement(15920..16639)
                     /locus_tag="ACHKW5_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7511665.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(16636..17070)
                     /gene="psiB"
                     /locus_tag="ACHKW5_002277"
     CDS             complement(16636..17070)
                     /gene="psiB"
                     /locus_tag="ACHKW5_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7511666.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(17125..19077)
                     /locus_tag="ACHKW5_002278"
                     /pseudo
     CDS             complement(17125..19077)
                     /locus_tag="ACHKW5_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            complement(19137..19370)
                     /locus_tag="ACHKW5_002279"
     CDS             complement(19137..19370)
                     /locus_tag="ACHKW5_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO7511667.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDEQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(19428..19955)
                     /locus_tag="ACHKW5_002280"
     CDS             complement(19428..19955)
                     /locus_tag="ACHKW5_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7511668.1"
                     /translation="MSARGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSREDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            complement(20392..20745)
                     /locus_tag="ACHKW5_002281"
     CDS             complement(20392..20745)
                     /locus_tag="ACHKW5_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511669.1"
                     /translation="MVTTSFRAAGTGDTGQAVEAACQPARNPLAGWRLPEGKRATLAR
                     RPLPFLLVTGVVSGSTCYRPVVKEPSYTCAPSAPGCLAASGRQARLRQAAACAPASLR
                     RTRWRGFGLPLRSDA"
     gene            complement(20708..20899)
                     /locus_tag="ACHKW5_002282"
     CDS             complement(20708..20899)
                     /locus_tag="ACHKW5_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511670.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(20899..21318)
                     /locus_tag="ACHKW5_002283"
     CDS             complement(20899..21318)
                     /locus_tag="ACHKW5_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7511671.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            complement(21365..21790)
                     /locus_tag="ACHKW5_002284"
     CDS             complement(21365..21790)
                     /locus_tag="ACHKW5_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7511672.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(22205..22975)
                     /locus_tag="ACHKW5_002285"
     CDS             complement(22205..22975)
                     /locus_tag="ACHKW5_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511673.1"
                     /translation="MNKTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            complement(23020..23487)
                     /locus_tag="ACHKW5_002286"
     CDS             complement(23020..23487)
                     /locus_tag="ACHKW5_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7511674.1"
                     /translation="MRALKIEKETRMYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSAAVMEIISNVSENRQVAVPAE
                     LLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            complement(23468..23689)
                     /locus_tag="ACHKW5_002287"
     CDS             complement(23468..23689)
                     /locus_tag="ACHKW5_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511675.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            complement(23690..24373)
                     /locus_tag="ACHKW5_002288"
     CDS             complement(23690..24373)
                     /locus_tag="ACHKW5_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO7511676.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(24450..24755)
                     /locus_tag="ACHKW5_002289"
     CDS             complement(24450..24755)
                     /locus_tag="ACHKW5_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511677.1"
                     /translation="MTETGGLPPVSFPVKDVAVLLFLLRRLTRRGINAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            complement(24759..25661)
                     /locus_tag="ACHKW5_002290"
     CDS             complement(24759..25661)
                     /locus_tag="ACHKW5_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7511678.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVLFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            26079..26327
                     /locus_tag="ACHKW5_002291"
     CDS             26079..26327
                     /locus_tag="ACHKW5_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7511679.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            26324..26761
                     /gene="umuD"
                     /locus_tag="ACHKW5_002292"
     CDS             26324..26761
                     /gene="umuD"
                     /locus_tag="ACHKW5_002292"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7511680.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRGEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            26761..28032
                     /locus_tag="ACHKW5_002293"
     CDS             26761..28032
                     /locus_tag="ACHKW5_002293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO7511681.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGCRLPEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCQQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPSYTTQWTEIPIASF"
     gene            complement(28037..28429)
                     /locus_tag="ACHKW5_002294"
     CDS             complement(28037..28429)
                     /locus_tag="ACHKW5_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7511682.1"
                     /translation="MDDERKRKKFTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGALQ
                     SAEKMPPSATDSVQEAMNDVRLKMQKLF"
     gene            complement(28434..29405)
                     /gene="parM"
                     /locus_tag="ACHKW5_002295"
     CDS             complement(28434..29405)
                     /gene="parM"
                     /locus_tag="ACHKW5_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO7511683.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKISKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLVPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            29634..30278
                     /locus_tag="ACHKW5_002296"
     CDS             29634..30278
                     /locus_tag="ACHKW5_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7511684.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
     gene            30272..30547
                     /locus_tag="ACHKW5_002297"
     CDS             30272..30547
                     /locus_tag="ACHKW5_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO7511685.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(30685..31173)
                     /locus_tag="ACHKW5_002298"
                     /pseudo
     CDS             complement(30685..31173)
                     /locus_tag="ACHKW5_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            31359..32894
                     /gene="cba"
                     /locus_tag="ACHKW5_002299"
     CDS             31359..32894
                     /gene="cba"
                     /locus_tag="ACHKW5_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin B"
                     /protein_id="HFO7511686.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLTDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVRSNPEIQEDGVTSETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISAVEILDSNSLSQRQEAENNAKDDFRVKKEQENDEKTVLTKTSEVIISVGD
                     KVGEYLGDKYKALSREIAENINNFQGKTIRSYDDAMSSINKLMANPSLKINATDKEAI
                     VNAWKAFNAEDMGNKFAALGKTFKAADYAIKANNIREKSIEGYQTGNWGPLMLEVESW
                     VISGMASAVALSLFSLTLGSALIAFGLSSTVVGFVGVVIAGAIGAFIDDKFVDELNHK
                     IIK"
     gene            complement(32912..>33427)
                     /gene="cbi"
                     /locus_tag="ACHKW5_002300"
     CDS             complement(32912..>33427)
                     /gene="cbi"
                     /locus_tag="ACHKW5_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin B immunity protein"
                     /protein_id="HFO7511687.1"
                     /translation="KDKNKKANEILYAFSIIGIIPLMAILILRINNPYSQVLYYLYNK
                     VAFLPSITSLHDPVMTTLMSNYNKTAPVMGILVFLCTYKTREIIKPVTRKLVVQSCFW
                     GPVFYAILIYITLFYNLELTTAGGFFKLLSHNVITLFILYCSIYFTVLTMTYAILLMP
                     LLVIKYFKGRQ"
BASE COUNT         8110 a         9258 c         8381 g         7678 t
ORIGIN      
        1 tcatttatat gagaaaaaga aaacaaaaga gggatgacaa tcccatgata tgggaaaaac
       61 ttgccatgct tactgcaggt gcatttttga tactggcagt cctgtggggg taataatatc
      121 ctgtttatta aagggcagaa ggcggtgccc ctctgccctt aagtacgttt ccgggattat
      181 cagtcaataa gttcccggat cagttccaga agagctatca ctgcattgac cacagcaaca
      241 actgccctga gaacgatgat taacaggtta atcatccgtt tctccgtcaa tggaggaaat
      301 cactgctgct tactcttaca ttgcctgtca caacagctct ctcattgtta gctgaaaaga
      361 gacttcctcc ggccagagca ccagacacag caccacctgt ttgtccggcc aggtctttga
      421 atagcacaga tccatagtcc cgatggattg atgcgaaggt aagtctccag acaaggctaa
      481 cttactgatt atacttgttt caggcgatat cgctactccc ggaataatag attgtaactg
      541 acagattgcg atcaggtgaa tataacgcta ttctggtaac gttagttgaa aagagacttc
      601 ctccggtcag agccaccaac tccaccccgg aatcgcaaaa aaaccggcct caccagtcgg
      661 tttttttgta cctgtctccc accaccttct gccgttcgct cttctctgtg aaaatgtcct
      721 gttgaactca ttcctgtccg gttattaccg gcgctgattc gcattcatgc cacccgactt
      781 aatcccgaaa ttgatccatt catctatgga ggaatccccg tgtcttacca acagacgtct
      841 gccgccgaag atccaatggc tatatggtat atcgtcggtg ccatatgcct gctctttgcg
      901 attatcatct ggcgcttttt acctgaaatc gtcttcgcct cctgcctgat cctgcacaca
      961 ctgtggggca tgattgactg ggggcccttt cacaactttg ccgccccccg ttacaacctg
     1021 ctggccatca ctgctaacaa cgcggcaacc atcactttca gccagtggct ggatgtaatg
     1081 tcccggacag tggggatcct ctggctgatt ctgcttccca tgacattcag ctttctgtgg
     1141 atgtggtttc accatccggc ccagccccgt tttacccgca ggccactgaa tatccacaca
     1201 ctgccccata ttttttcagc tctgtcaccg gcgatcgccc ccgttctggc tgacggagac
     1261 aacaacagac tcttccacgg acaaaaacgt cccgagcgcc gtgtcgcact aacccccgaa
     1321 gcctttgtgg agcagaacaa ccttatccgc aacatgcagc tcgatgttgc gtccacacgc
     1381 cagtgcttca tagcgcaact ggggcaacca ctgaccagct ggaaagacat ggccccacat
     1441 gaaaaagcgc tctttgccat ctttggcctg cagttcttcc tcggtgaccg gaaagcagcg
     1501 gtagccctga tgaacaacct caatctctcc tgcaggctca aaagtaaacg ggaccagggg
     1561 cgtttcagca cgccggtgta ttcactggcc agaaacgcct ttatccgggt catcaaaaca
     1621 gaaggcgcac aaaagtggct caggcagcac cgttacgttc gcagcggtct ggtctggctt
     1681 tacgcacatg atttgcgcct gacgcctccg aactggctgt ggctcaaggg cgttgaccgg
     1741 acgctcttct atgcccttca ccgggcaaac accacaaaag gcttcatcga aggggccggc
     1801 gtggtggccg ttgcccgggc agaaaatgaa gcgtgtcgtc tcggcctgcc ctgcccggaa
     1861 ccctgtgtgg aagaagccat tgaagggctg cggcaggaca tgctgagact ggggttgata
     1921 tgggacgaac ctcagccaga ccgcgatcgc agacgtcaga taaggacccg ctggtcactc
     1981 acggatgagg tgataccacg ccgccatgat aacgatgaag gctcagagac tggtgaaacc
     2041 accgaaacca ctgaaaccac tgaaacccgg catcccgccg acaaggaaaa agcacaatga
     2101 ccatccgcta cactgcaaca ctgaacaata aacacctgtc cctgaccgcc ctgtaccctg
     2161 acggtggtca ggaggttatt gccatcctgc cggttacaga actgggcgca ctgattaacc
     2221 gtaataacag gctgatgcac ctgtccttca gcaccccttt tcgtgaaata accgaaacac
     2281 tgcttgttga taccgttgat actgaacgtc ccggacatca catcggcaga atgctctccg
     2341 cgattgacag ctgtctgaac gcggcatatg cagaacttcc tgcccggaaa accagcttca
     2401 tgagagggct gatatacggt gcaggattag tgctggcact tctgacagcg gtaacagccg
     2461 gatatatttt actgaaaccg gacagcgatt cccctctcag ggtacctgtc acagaatccg
     2521 taacagaaac tgcaccatta cacatcgaca aggaaattgt ccggtcagta gccccgtcag
     2581 agacgaaaaa tgaaatcgtt cacccggcac cacaggaaat gcaggagaca tccgctgccc
     2641 cggcgataac agcagacaaa aatactctgg tcactgaagc ccgccgtaat ctggccactg
     2701 tacttaaacg caacgcggac cggggtatgt tcaccattaa tctctccact ggtcatgaac
     2761 gcacactcta cgccttcctt gaccccgcat gcatcaactg ccgtcttctg gagcccgcgc
     2821 ttaagcaact ggcccgggaa ttcagcgtgg tgatttatcc ggtctccgta atcggcggag
     2881 aggccagcac aaaccagact gcgccactgc tgtgtgaacc ggacgcacaa aaacgggcga
     2941 ccggatggca tcagctgtat tccgcagacg gtggtatggc acccaccggc gatgcaccgg
     3001 ctgcaccgga tgaaaagtgt ctgaccgctg ctcgtgctgc catcgatgtc aataaccagg
     3061 ctttccgcca gttcggtttt ggcggtaccc catgggtgtt atccgatacc ggctggcacc
     3121 tgccgtccgg cattctgaaa gacgccacta ccgtcagcct gttcctgaaa gccacagata
     3181 ccgaggataa tcacaatgag ccagcatcac gttaatcctg acctcataca ccgcacggcc
     3241 tggggaaacc cgctgtggaa tgcactgcac aacctgaaca tcactggtct gtgtctggcc
     3301 ggcagtatca ttacggccct gatatggcca ctggcccttc ctgtctgtct gctgttcacc
     3361 ctggtcacct ccgtgatatt cacgctgcaa cgctggcgat gccccctgcg tatgcccatg
     3421 acgctcagcc ttgatgaccc ctcccaggac agaaaggtca ggcgaagcct cttcagcttc
     3481 tggcccaccc tgcttcagta tgaagcagat gaaacatccc ccgcacgggg gattttttat
     3541 gtcggttacc gccgtatcaa tgatatcggt cgtgagctct ggctgagcat ggatgacctc
     3601 acccggcatg tcatgttttt tgccaccacc ggtggcggta aaacagaaac cacctttgcc
     3661 tggctgctca acccgctctg ctggggaaga ggctttacct ttgttgacgg caaggcacag
     3721 aacgatacca cgaggacaat ctggtatctc tcccgacgtt ttggcaggga ggatgatatc
     3781 gaagtcatca acttcatgaa cggcggaaaa tcccgcagtg aagtcattca gagtggcgat
     3841 aaatcccgcc cacagtcaaa cacctggaac ccgttcgcat tcagcaccga agccttcacc
     3901 gccgaaacca tgcagtccat gctgccccag aacgttcagg gcggtgaatg gcagtcacgg
     3961 gcgatagcca tgaacaaggc actggtcttt ggcacaaaat tctggtgcgt ccgggaaggt
     4021 aaaaccatgt ccctgcagat gttacgggaa catatgacgc tggaagggat ggcaaaactc
     4081 tattgccgtg gccttgatga ccagtggccg gaagaagcca tcgcaccact gcgaaactac
     4141 ctgcaggatg tcccgggatt cgatatgtca ctggtgagaa cgccgtcagc ctggactgag
     4201 gagccccgta aacagcatac ttatctctcc ggtcagttct cggaaacgtt cacaaccttt
     4261 gccgaaacct tcggggacgt ctttgcggca gatgccggcg acatcgatat tcgcgacagt
     4321 attcacagcg accgcattct gattgtgatg atccctgcac tggacacatc agcccatacc
     4381 acgtcggcac tcggtcgtat gttcgtcacc cagcagagca tgatcctcgc ccgtgacctg
     4441 ggctaccgcc tggaaggcac agatgcacag accctggaag tcaaaaaata caaaggcagc
     4501 ttcccctata tctgtatcct tgacgaagtc ggtgcctact acacagaccg cattgcagtg
     4561 gaggccaccc aggtacgttc actggagttt tccctgataa tgatggcgca ggatcagggg
     4621 cgaattgaag ggcagacctc cgccaccaac accgccacac tgatgcagaa cgccggcacc
     4681 aaattcgccg gacgcattgt cagtgacgac aaaacggcaa gaacggtgaa aaatgccgcc
     4741 ggtgaagagg cacgggccag aatgggcagc ctgcagcgcc acgatggtgt gatgggtgag
     4801 tcctgggtgg acggtaacca gatcaccatc cagatggaaa gcaaaatcga cgtgcaggat
     4861 ttgatcagac tgaatgccgg tgaattcttt accgttttcc agggggatgt ggtgccgtca
     4921 gcctctgtgt atattccgga cagcgagaaa agttgcgaca gtgatccggt ggtcatcaac
     4981 cgctatattt ccgttgaagc cccccggctg gagcgtctcc gccgtctcgt ccccaggaca
     5041 gtgcagcgca ggctccccac accggagcat gtcagcagca tcatcggtgt actgacagca
     5101 aaaccatccc gtaaacgtcg taaaaaccgt acagagcctt accgtatcat tgatacattc
     5161 cagcgccggt tggcaacagc gcagacatca ctggatctgc ttccgcaata tgatacagat
     5221 atcgaatccc gggcaaacga actgtggaaa aaggctgtgc acactatcaa taacacaacc
     5281 agggaagaac gccgggtatg ttatatcaca ctgaatcgtc cggaagagga acactccggt
     5341 cctgaagata taccttcagt gaaggcgatt ctgaataccc tgctccccct ggagatactg
     5401 ttaccggtcg ctgaaaataa taaatcgcag acaataaata atgatcatcc ccagaaatca
     5461 tgtggagcac aaccaacaac tgacgaaaaa tactgaagga atacaaatat tttctggtga
     5521 ccatatctgt caatcagact gtaaaaccct gttcactaag tccggggttt tacggcgcac
     5581 tggataaggg ccgcagacac ggcccttaat tcagagttaa ccaggtataa actcaattca
     5641 gtgactactc ccgcccttat tgacgaaggt tcctgccgtt tttgggctga atctttgcac
     5701 catcacttat ccgggcttac attccaggtg atggtggctc ccatgttttc tcctgccgta
     5761 acgtataatt atcatcatgg ctctccgata aacgggtctg ctgtctgata tattcccggt
     5821 aatactgctc actgcgttga cggtgcaaat ccacgtccgg acgagtaatg gtgaagtgtc
     5881 cgtttccggt tattcccgct gcattgtgcc aggccaccat ttctgcaaac acatcattaa
     5941 actgctggta tgaaccttcc ggctcaaaac caagctttcc gttgcggctg aacagaactg
     6001 gcgcaattaa atcaagcttc tctgccagtt catttcggtc cgtgtgatgg tagaacacaa
     6061 cccgatctcc gtaatccgta cagacatttt ttcctgtccg cgtatccctg aaatgaacac
     6121 ttccattttt ctgaagacga gcttcaagtt ttgcaacatt attaaaaagt ggcgttcctc
     6181 cgggttcgca gagaacgaca caacgatcag cattagttgt acgtctttta tccttattac
     6241 gcgagcgacg atcgcgataa tcccagcctc ggagctgtga cactgcagct ctgtcgcctt
     6301 gcgccgcctg ttgttccacc cactgccggt aggacaacgg ataccactta ccctccgcca
     6361 tcagcgccag tcgctcttcc ctgacatttt ccttcagtct gatcagcgcc tgcatacgtt
     6421 gcacttctgc aatatggtaa tgcagtttac gcacttgcgg gtcccgaaac tgcacgcgga
     6481 tatgagcctt gcgacgacgg caggcagcat gaatttcccg caaccgttcg ccgtaacgta
     6541 aatcgggttt acgccagtgt tccttccatg caagataacg cgcacgcaaa tcttcccgcg
     6601 cagctgcacg ggcttcacgg cgctcacgac gcagctcagg atcgcgccgg aagccaggct
     6661 ccacttcatc actggcagca agttccggat tgtagcgaca tttaggcttc acccgttcga
     6721 aaatatctgc agccgcaatt tcaaatggcc cggcctgagg ctcggcccgg gataacgtca
     6781 aatccgggtg gatactgctg gccttcaccg gtgtcagttc atgattaaac gcatccacaa
     6841 tcacgagacc atgatgctgt ttctgcagca taagcccttt acgtgcaaac atctcatgac
     6901 atgcctgcca gctgaaatcc ccgccagcta tcatctcttc aatctggctc ctgcactgaa
     6961 taagagcgta tccccacaga ctttccttat cggaaaacag ttcacgcttt atggcaccct
     7021 gtggaccagt gcccatcctc atgcgatacc attcgtaatc gctgacagcc tgagtgccaa
     7081 tactgtgtcc ccggaatcgt tctccgggta taacctgagt aaaaatatcc ttcggtacag
     7141 agtgccatcc accatccaga tcctgcatat aatccagcgt cattaacggc cacacactgt
     7201 ctgcacgtac tggtgtctgt tgtttatcaa atccatcatg cagtaccaga tgcccgtgct
     7261 ggatccccat ccataatcca gcctttgcca gagttctgtg cacatccagg caactttcca
     7321 gctcaccatt tctcgccatt tccggtaagc gttcggcaaa atgacggcag gcgtactgtt
     7381 tcagactctc cgtttctgcc actttttccg gccggatatc aacgttaatg gcttccggat
     7441 cataacgacc gggtgtcccc acctgactga aaatatctgt cggtacaggc tgatattccc
     7501 ccagtttccg ccccagtttt tcagctgtcc atgacggtcc gaacgaactg agtgcaacac
     7561 cttcccgcgc tctgtcccag ccatccttca ccaccagttc cccttcctgc atcgtgatat
     7621 acagaccatc actggccagc cgtttatgca aagataacca gtcttgtgct ggctcttcac
     7681 gcagcccggc aatcgacatc tgagcaatat attccctgaa cgtctgcttt ttcccgcgtc
     7741 gccaggcatt acggcgctca cgtaccagcg ctgttttgcg cacaatacga ttgcccggtg
     7801 catgtacaaa gcaaccatta tccggcgcga atccgtgctt aagttcaagt tcacggcatg
     7861 cacgactgag tttttcctgg ctccacggaa ggcaattgat atagccggtt tcagggtgga
     7921 cacgattcac tgcaacatgt acatgcaggt tatccgtgtc tgtatgaacg gctgccacat
     7981 actgatgttt tgacagctca agggatttca gggtgtgacg cacacagtca aaaagctgct
     8041 cagaacgcgg actttcgtgc gcaggccagg agagaatata gtgaaaaaca gggtctgtat
     8101 catctttgga aaaatgagca agctctgctg ctttctgcat ttcttcagcc gcagattcaa
     8161 gagacaaaca gttatgaaag caggtcacac catagaaatt cacccactga ctgccgtctt
     8221 tcatcacatc aatcagggaa acaaattttt cattacgcag acgtgttgca taatcaacaa
     8281 gacgtctgaa gcggttccgg tgcggaatct cagctgtcat ttccgtgtct ggcctgagtt
     8341 catcttccgg cacatcatcc cgcactgagg tgtacgcaat caggtcttca aaagaagact
     8401 ttccgtcgcg tctttttttc ggaatgatgg cattcatcag cgattatctc agcgtcccat
     8461 taaatctctt cgcagagtat tagcaagttc tgtaatagca acgagaacct ctgcatactc
     8521 tttatctcct gtgcgttttc cttccacaaa cagatgcttc tgcaggcgac caagacgcat
     8581 cagctctttc aaaaaatctt catcaatgcg ggcatttatc tgttttccca gcacagtttt
     8641 tcttatcaga gttgaaactg tcagtccgtt tttttctgcc ttttctttaa caatctcagc
     8701 ctcttctggt gaaaaacgaa cagcaatgag gacattcttt tgccgtttct cactgcctct
     8761 tcgggttttc ttctcactca tggtctgctc cttaccgggg tgtcggggct aagccctgac
     8821 caggtggtaa tcgtatagcc gtgcgtgcgc ggttatacga ttacacatcc tgtcccgttt
     8881 ttcggtccat tatagcctgt tatttttgtg catcatagtc ttatgcaggc ggcagaggca
     8941 gggcgcagcc ctgccaggag tcagtttttg atgataaaat cagacttcaa catgaaacgg
     9001 agagaaagtg ccaatggctt ctaaaaaatt ttactcagat gatgacatcc agctggcaaa
     9061 agctgcttta tcagaattgc cggacctcac cgctcaacga aaaacattgc gtgattttct
     9121 tgatgccatt cgtgatgaca tcattgtcct ggtaaggaca aagggttata cactggcaga
     9181 tgttcgtgac acgctcaaaa atgcagggta tgaagtagga gagaaggcac ttcgtgacat
     9241 tattctggaa gcagaatcga aaaaaccatc ccgccgttct tcctctaaaa cagcacctaa
     9301 aaagacagat tcaggcagga aagatggcat cgatatgaac aataattgat gtgacaatgt
     9361 cacatcgtgt tctgagatta tgatgattac agaaaaaaag ccctggcata aataccaggg
     9421 cttttctgtt taatcagact cgtggcggca atgcaccagg ataatgccgt gcaataatat
     9481 cattcagttt ttcaacaagg tcagaacgct taaagacaat gtgtacggtc cctttctggt
     9541 agccacgcac cgtaaaatat tccccttcaa acaactcacc gacattgtcg ccgttcccga
     9601 gaaattcacc atactgcgcc ccgatgctgt tcctgaagtc aggaacgttg cgcccctcca
     9661 gcagataaaa cggacgtacc aggtcatcca gtgcctcccg tccgctgcag tccagcgtca
     9721 cacgcccgtt actccagcgg tacagcaggt tttcaataat gattttcttt cccagacggc
     9781 agggattatt ggttctgtaa tcccatgaca atttccggta gacatcaatc agtccatgtt
     9841 caaatgtctg catcttactg gcattcagat ggcgaaaagt tgccagtaca ttgtccaggg
     9901 tgatttcagg acaggtgtcg ctcatcagct ggctgttcca ctcatcacgc tggcatgaac
     9961 tcatgaatgt gtacatgcca gtatccgtca tcaggagatc ccagattttc cggtcggcaa
    10021 gacgggtgat attctttttt gcttcttccg gtttatccat gcatcgaacg gagtcagtca
    10081 cgcagtcttc aaacccgtag agaaaaatgt caccggaagc atcgagcaga atcctgcgcg
    10141 ctgtcgccag gcattccatc gcctgcatga acagtgcaat accttcgtta cgctgcgcga
    10201 tgatgcggtc gatagccaca gacggaataa catctgtcgt gccggcagta ttcaggtcag
    10261 cagtgggtaa agtctgattc atcacattgt ctcctcatcg tcacgactgc cgggtcagcc
    10321 aggtcatttc ccggaggagc gaagctcctt ctgccgtccg ggtcttcggg gatttccccg
    10381 cagtggacct ttccgatggg ggcccgttta ccgcctcacg ggacagctgt ccgggggcgg
    10441 acgcaaaatt ttttgccggg ttcatggcta aaaattttgt ggccggtgca tctcaccctg
    10501 gacagatgga acgggagggg gttacgtctg ccccccggca agtccacagc gggaaacagg
    10561 ccgcagaggt gacggcagga gggaagactg ctgcgccatc aggcgcagtt tcctcacaca
    10621 ccgtcaggtg tgcagggagc gcaggtcacg gcgcgactga agaaaaacga cggcacaaca
    10681 atggcatgtc gtggtcactg gcgcaccact cccgcacagg tgaaaacaga gaaaacaaca
    10741 cgacatatac aatcaaaaaa aagtgaaatt tcttcagttt ttcactcttt ttatcgcgct
    10801 aacttggtga ctatgatgat aaaataaagt gaagtttttt cactttttca aaggtgtttg
    10861 atgaccggct gggaactgcg aatctggcga aaaagcatgc tctggtcacg cgaaaaagcg
    10921 gccagagaat ttggcgtgac gcagagaaca tggcacgcct gggaaaatgc ggaacaggtg
    10981 gacgtcaccg tctggaggac cacccaggca ctttcagtcc gtgacctgct gcctcacatg
    11041 caggggatgc gcaaggcaga tatcatcagg cgtctggaaa atgaactggg ggaaacggca
    11101 gggaatgtgt gagcgtgatt cacagcctgg cagaatgcca ggctactgct catcaggaaa
    11161 cagctttttc agttcttcac cggaaagtcc ggttgcctcc tgaatccgtt ccggcgtcca
    11221 gcccatatcc cgcagccggc aggctgcttc ccgcgccact tcaagcgcac ctttcttaaa
    11281 accttcttta aaaccttctt taaaaccttc gtcgaaacct ttctgtataa gacgttctgc
    11341 aagcgtcatg ctgtttgctc tgctcctgct ggaaaaaagc ccggcatttt gccgggctgt
    11401 gatgctctca tcagtgaatg atttttttca gttcagcttc ggaaagtccg gtagcctgct
    11461 taatctgctc cggcgtcatc cccattttct gaagctggca cgcaatagcc agggcttttt
    11521 caagcgctcc ttcctggcga cctttttgct caagacgttc tgcgatagtc atcagtttat
    11581 cctcatgctg cggcagacgc tcagccagcg cccgcataaa ttccggtgat acagagtccc
    11641 ctgcgttaac gatgtaatga atcagaacac gcacctgctc gtctgaaatt tccaccatga
    11701 cctgcgggag tttgtccagc agctccatca tgtcacgatg gcggatatgc ttcatcagca
    11761 gcgtcagtgc ggccatccgg cgatggttca tgatttcatt gtcatcaacg acagtgatat
    11821 caaccagcgg aaacggcttt gtgtatattt tagccgcaag tgccggattc tcaaaacagt
    11881 ccagccagtt catgctgtac gggtacgggc tgcgctcacc atgatagaac agcaccggaa
    11941 tcaccagtgg caatgttttg tgctgctcca gatgtctctg catcgccgcc acactgtaac
    12001 gtaacatgcg gaaaggcatg tacagatttt cggtgctctg gtgctcgatg agcgtataaa
    12061 catagccgtc ttcacccccg gtggttttca cactccagag gatatcgctg gcgtactgat
    12121 gcaggtccgg ctcaacaaag gaggcgggtt ccagcttcag cgtggacagg tcgcacaact
    12181 gccggtactc cgccggaaga tgcagttcca gaaaatctct ggcgacgtcg gggttcgcca
    12241 ggaacgaccg gaacgcggca tcatgcggcg tgggcgttgt ggtgtttttc ttcttactca
    12301 cggttcctcc atcaaatcag cagcatcgca gcaatcatcg tccaaatccc cacaaccaag
    12361 aggacagcaa ttgcgatcaa agccgtgaca acaatagcca cgttctgcgt cacggcgtat
    12421 ttcttctgca atctcttcag cggaaaggta atcaaactcc tcacgggcca gttccaggac
    12481 cagcagatgt tgttctgtcg gcaatgtaga cacaaaatta tcaatagcag cactcaaacg
    12541 tcgctgatat agctcataaa caagaccact acggtgcatt agttcttcgg ataatagttc
    12601 cagttttgag cgaacgctgt cagcattgaa agtgacttca tcagatgaca tctgaaaatc
    12661 tttgtcagtg tcaaaacaag acttcatttt tatctcctgc ccgtccgggc cctgaatgaa
    12721 agagaacctg tttcccttct ctgttttttt atggaacagg aggaatccgg taccatgcga
    12781 catagacgtc atgatgtttt tcatcttgct cacggttcct cctggtaaag tacctgaaca
    12841 tatcctgcca cctgcacaga cagacggcag ggggtaatca gtggcagacc cgttcaattt
    12901 caagcgcacg gagcaggtgc ccccactcgc tgactttcat ggcggggata cgtacccgct
    12961 tctgctcact gcagcgtctg gcgatatgac cgcccatcgc caggacgtcc gggttaatct
    13021 catccggtgc cagttcctgg cgtctgaagc gggtatggcg gtgcaggggt ttgttctccc
    13081 gcaggcactc ttcacgcaga cgctttacat actcggcggt tgcgaagata attgatggtc
    13141 tcatggcata atctccatag ttagtgaatg agatgccgga cttccgtacg gggaagcgcc
    13201 ggcggagaaa ggcagggttt atcgccctgc tttttttcat tccggacagc gacgcagtgc
    13261 cagcgcgagc gctttcatct tccagccttc cggcgtgcgg tacatcacct tccagtagcc
    13321 gtcgatgaac acataatgga agcgcacctc ttctgtgctt tccgtggcaa tagcctccat
    13381 gaaagaacgg tactcacgcg cctcactgtc catccggaaa ccactgtcac gtccgtaata
    13441 agccgtcaca ccgtccaccg ggttctgaag cgtatgcccg gcgggtttgt cgcaacgggg
    13501 accaaggcaa cggatatcac cgtgttcaac cagggcttcg gcctgctgac cggtggcata
    13561 gtgggtatgc aggatgcgac cgttccaggt cggctcaccg tgcaggtggc agtaaacggt
    13621 acggaacacc ccgtcgcggc aaaaaacgga aatcgtgctg ctgactgtca tgaaaacgtc
    13681 tccttctgct gaatcaggtc gccgggccga ccggttgctc ccccggaggg gcgaaagccc
    13741 cttctgccgt ccacttcttc ggggtttctc cccgcagtgg acctttccga tggggcagcg
    13801 acagccacgg cacggggcag ttgtccgggg gcagacgcaa aagtttttga cgcttgcggc
    13861 aaaaagtttt gtggctggta catctccccc tggacggctg acacgtgttg tggccgcttc
    13921 agcccccgga aagtccacag cgggaaacag gccgcagggg tggcggcgga agggcagaca
    13981 actgcgccat caggcgcagt ttccttacac accgtcaggt gtgcagggag cgcaggtcac
    14041 ggcgcgacag aggcggcgcg accggaacgg tcgctgcagg ccaggttttt cagacatcgg
    14101 accgtagcgg cagcccgaaa ccgcgccagc gggttcggcg gagactggcg ggtgcgccag
    14161 cagctgcctg tcggagtcgg gcctgacgac cggatgccgc caggcagccg ggagccgaag
    14221 gtgtccccgc ctccggaaat cagaacaaca gaaacgacat aacagaaaaa tgcctcacac
    14281 catgacgggc aggcgtaagc ctgccagggt tttccggggg cctggcaggc catacccccg
    14341 ccggagcggg gagtaaatca ccgacggaac acatgaccgt caataaaggt gaaggagtcc
    14401 aggaataaat cccgcgcata cgcctcatag tcaaaataga gcgccaccgt ctccggcacg
    14461 tcagccagca ggccggtatc actgacgaac tcaaccgcaa aagcctcctc actgtcagcc
    14521 tcgccccacc aggcatcgcg gaagctgtca aaatccgtct caccggtatc atccacccag
    14581 agacgataag cctcttcgca gccttcatcc cgtgcctggc ggaagccttc aacccaggcc
    14641 cagttgatat gacattcaga ggccatattc cccgggaatc cctcataatc ctgaaacatc
    14701 agttcaggat cggcttcatc ctggtgaaga gcacggcagg cggcgaaaaa gtcgcgctca
    14761 tcatcaaacg tggtcaggtc aaaccagcgt ccggcgatgc ttccacagtt gtatttgtgc
    14821 caggttccaa cgtatacagc aggtgcaaca acagacattc ccgaatcctc catagtcaca
    14881 tgttctgcga caccgggcgg tgccgtgcct ccagaaaagc cgggcgataa ggcggggatg
    14941 caagggcgca agggaagccg cagcccgacg cgcacggctg acgggagtta gagggtgaga
    15001 ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg
    15061 gtgaccgcag cggttcatct taccttgcat cccgcccgca gaagcccggc tacgatggag
    15121 gcacggcaca gcccgcacac gacgctgagg ccacataact gaaaacccac aaggccatgc
    15181 cccgcatggc cgggtaaaag cggagccgcc ggcgacttcc ttattccggt gagagcagag
    15241 gacacgccgc atgacctcca gaccacccct gatatgaaac gatcatgacc ggcatgtccc
    15301 tgcccctcaa gcatacctcc actggttcac cgaagaacat ccgtgatgct cacaagtttt
    15361 ctgtcgcgca gccagtcctg cgcttcacgc accacacaac tgtccggttt caccgctttc
    15421 agcgatgatg ccagtcgctt gatcaacatc accagttccc gtgtctgtat gtccggcatt
    15481 gtctccggcg gagcagaggg actctcattt ccgacgccca tcccatgcgc ttccagtgtg
    15541 gcacggacaa accaggcagc atcgaccagt tgctggcgaa tccaggccag atcggcattt
    15601 ctgtcacggg ctgccattcg ctcacccgac agcgccttgc tgatatggct gcgcaccaga
    15661 ttatccagga gttcctgcgc ttcttccggt gaaaagccac ctggtatctc tgtgcgcaac
    15721 agtcctttcc tgtacagtag ctgcagcaga tcaatctgcg accgcactgc aactatcagt
    15781 acataacgga caatattcgc actggcggtg gtttctgtat cattcagcag ttcctccgga
    15841 tcattaccac cggtattgca gagcaacggc aacagaggtc gcatccacac gggaacctga
    15901 tattgttcgt ctgatttcat catattgcgt tctccagctt gttcgggatc atccactgct
    15961 caagtgcacg tacctgtacc ggtgcaccac gaccggcctc accggcttca aaaatcagcc
    16021 gccagagtgg ttcctcctgc cagaaaaacc ggtcaaatga tgacagtgat ggaaaacgcc
    16081 cgcaccaccc ccagaaaatc gtttccagat catgctcctc aacaacacca gaccagcggg
    16141 acaaccagct tccgaccgtg gcgggagtat gaaacgcaag gtcaatctgc gcctgagcaa
    16201 aacatgtctg gtattccagt tcccgtcgtt ctgcctcacg ttcccgctgg cggctgtatt
    16261 tctgctctgt cagcgtggtt ttctgacgac gacgctcacc ttcacgaaaa ggaacggacg
    16321 ggaacagacg atgccccgca tcggatggca ggggaagaac acagacttca ccaaaacttt
    16381 caatcagttt atccacggca gccagccaca ggagacggtt gccatgaaat tcctccttcg
    16441 tcagcgcagg ggcaaaaaag cggagatcgg tcagcgaaat ccggcgactg ccattcaggc
    16501 agcgaaagaa agcgctggca taaggccatg ctgaaagtgt gcgtccctga cgacgacgtt
    16561 gttcggtgac agccacagcc tgcatggcgg cctgttgttc tgcgctgagg gggatcagtg
    16621 cccgtgaacg tgcgctcatg cctgacctcc ttctgccatc aaaatatgaa tgatgtcgtt
    16681 aacactgtat ccctgcgcat caagtgtcgc ggcgagcgcc agcgtgtgac tgatcgcgac
    16741 aggcgtgaag atatgttgca cctgaacgac ggcgaaaggc tgtccaccac cattcaccag
    16801 aaccagcatc cagagttgag agacgtctcc cggcgaacat aatgccaggt ggaaacgttc
    16861 gtgggctggc gtgaaacgaa cctggacggg gaaaaagccc ccgaactcac tgccatactc
    16921 gccattcatc atccagttag ccggtgcgtc cacttcacgc atcacctcgt gagtgagatt
    16981 acggcgcata tcgcttcccg cagcacggat atcttcatat tcctgtgcgt tcatggactg
    17041 cagggcattc agggtcagtt cagttttcat cacggtatct cccttgctgc cgccccgtct
    17101 ccggcggggc ggtgtggttg ttcatcaggc ggcatcagcc aggttagtgg tgtgttcagt
    17161 ggcatccatc tgtggacgtg gtgtacacat ccagccaggc acccagcggt tgtctttcat
    17221 atggaactct gcatgttcag ccgcatcgcc tttcttcatc ttctccgcgt cccgtgcggc
    17281 accggacagt cctgcctcat tcagggctgc gataatctgc ggctttttca ggtgtccgaa
    17341 gaagtttgct tttgtcggct gccaccagtc gcgcatgtga aaaccgatgg cgctctccag
    17401 cgtgtccagc gggctgcgtg acgtgtgacc acactcgcgg gtctgaactc cgtggatact
    17461 gcacgcggtg cagaaactga gcagggataa cagcacctcc tgactgagtg aaaggaacgt
    17521 cgtcatgtcc cgtgaccatc cctccggtaa cagggccgca agacgggctt tttctgccat
    17581 catcgccatg aacgcggcac cttccttccc tgacggcgca tcgctggtca gcgaataatg
    17641 tttacattcc aggctgattt gtgctggttt actgtacgct ccgctgccaa acacattcag
    17701 gcagagcgtc catgccagca gtgccagaga tttgtccggt tgctgcatca gtgctgcctg
    17761 gactgccagc gtgcgttcgg aagacatttt cgtcagcagt ggcagactga tttcctccac
    17821 gggcgctttc tcctgcacct gttccgtgcg gtcagcgtcg tcagtcgcgt catcctcact
    17881 gcgcaactgc acaccacgct ggacacacac attgccataa cgccaggaca ccaccacacc
    17941 gcttccggca cgcatctccg gcgtccacgc cctgaccttc gccatgcagc gcatcagctt
    18001 catttctgct gccagcaggt cggattcctc acactggttt tccagtgcgt cgtaacgtgc
    18061 catcagttcg ttcaggcatt cttcttctgc ctccgtcagc accgcttccg gctccggcag
    18121 acagcggtat gttccggcat cctcacggca ctcaccgaca ggctccatac gtccggcgca
    18181 ccattcccag ccttcggctt cccgaaggtg ttcagcgaca gcctggagtt tttccagcag
    18241 ggcagcatcg agcgccacgc ggtcgacata accgtccccg tcatcgctga acaaatcagt
    18301 acgcagttcg tcaggtgaga aagcatcagc ccccacgaag cggaatttac tgttccccgc
    18361 caccgccact tcactttcgg tcaccagacg acgaatggtc tgcacttccg gttttccacc
    18421 ccatcccgac tggcaggcgg cttcaaacac ctgcacctga cgcgcggtgt cgttctccag
    18481 cgcctgacag tgctctgtgg tgatgcggtc ttctgccagc gcatcgagta tgacaggcac
    18541 aaggtctgcc agtttcagca ttcgctgaac gtggcggggt gaatagccca gcaaatcacc
    18601 gatttgtgca ggtgttttgc cttcctgcgc cattgcacgg aatccggcaa tctgttcggc
    18661 agggtgcata tcccgacgat gaccgttttc agtcattgat gcggcagtcg ccagttcctg
    18721 cggaataatt ttcacgcgga caggccagtc agccggaatg attccacgct ctgccagcat
    18781 gttgagtgcc gccagtcggc gaccacctgc ggcgacaccg taacggtcac caggcagagt
    18841 atgcacgacc agattctgca acaggccaac gcccttaatg gagtccgcca gttcgctgac
    18901 ggactcagca gaatacggca ccgtgcgcac attcagtggt gatttaatca gttaggccag
    18961 cggcacgctc acttcctccg tctgcgccag cagggcagac aggacagttt cctgcgtttt
    19021 tgcaggttta cggcttgctt tacgggtaga ttttggttca gactttgtta ctgacatgac
    19081 tatcccctta ttgtgtagtt aatgtctgcc ggacgcgttc ccgtccggca cctctcttat
    19141 ttaagaatgc cgaaatccct gatgacatgg ttcatccagt acccggcacc gtcctcaaaa
    19201 ttccaggtac gccagaccaa gccactgtta ttgcggacaa ccaggcgaaa atgcgttccc
    19261 tgctcatcct cgataaagac gttccggtac tgtgcggcaa cggcttccgc ctgttcgcgg
    19321 gtaaaggagc cgtccggcag cccctgaagt attctgaaat attcgctcat cctcatctct
    19381 ccggtgatgc cccgcgcagg gcggggcggt tatcacagtc aggccagtca gaacgggata
    19441 tcgtctgaaa acccgtaatc ctccccctcc ggcggttgcg gctgaggctc gggctgcgcg
    19501 gccttacgtc cacggccttt cgttttcgcg ctcccttttt tcgtcgcgtc agcactctgt
    19561 gactgaccat tctgctgagg ctgcggttgc acctgagcgt tctgctgtgg tgcactcccc
    19621 agcatctgca tggtgcccgt ggtcttaaca agaatttcag tgacgtaacg ggtgataccg
    19681 ttatcttccc tgctacgggt acggagttgc ccctcgatgt agacctgcgc gcccttacgc
    19741 agatattcac ctgccacttc cgcgagcttg ccgaacagca ccacgcggtg ccattccgtc
    19801 tgctcccgca tctcccccgt ctgtttgtcg cgccaggttt ctgacgtggc cacctgcagg
    19861 tttgccactg cgcccccgtt ggggatgtaa cggacttccg gatctttacc cagacgcccg
    19921 acgaggatga ccttgttgat accacgtgca ctcataagaa cctcctgact gaaagatgtg
    19981 agataaaccg gataccggcg acgggacgtt catgtgcccc ttcagaggag cgaaagctcc
    20041 tcctgccgtc cacttcacag gggtttctcc ccgcagtgga cctttccgat gggggcccgt
    20101 ttaccgcctc acgggacagc tgtccggggg cggacgcaaa attttttgcc gggttcatgg
    20161 ctaaaaattt tgtggccggt gcatctcacc ctggacagat ggaacgggag ggggttacgt
    20221 ctgccccccg gaaagtccac agcgggaaac aggccgcaga ggtggcggca ggagggagga
    20281 ctgctgcgcc atcaggcgca gtttcctcac acaccgtcag gtgtgcaggg agcgcaggtc
    20341 acggcgcgac agaggcggcg cgaccggaac ggtcgctgca agcccgggtt ttcacgcatc
    20401 ggaccgtagc ggcagcccga aaccgcgcca gcgggttcgg cggagactgg cgggtgcgca
    20461 agcagctgcc tgtcggagtc gggcctgacg accggatgcc gccaggcagc cgggagccga
    20521 aggggcgcat gtgtaagaag gttctttaac gacaggtctg taacaggtac ttcctgaaac
    20581 aacgcctgtc accaacagga agggtaacgg gcgacgcgcc agcgtggccc gcttcccttc
    20641 cggcaggcgc cagcctgcca gggggtttcg cgcgggctga caggcggcct ccaccgcctg
    20701 ccctgtatca cctgtgcctg ctgccctgaa ggatgtagtg accaccgagg aaaaccgcct
    20761 gctgattcgc cacaaaatca cagatttcat caacaatctg cgcggtggtg agcggtttct
    20821 gccccatttc acgacgccgc gcattaatca cggccctgta attacgcaga atttcgcggg
    20881 tttccgtgct gatattcatt acccgttctc cccgtccagc gcctgacgca ttacccgcat
    20941 aatctcacgt tcttccctga caaatgcgtc accgtcgccc ccgttctcgc tccctacagc
    21001 atggaggcga taaatttcac taccagccat caccatcact ttttccagca gcaccgcagg
    21061 gactgttaca agacggttta cgcgcttttc ctccatcagt tcattgacaa tatcggaaac
    21121 aactctgtca catgcgccat atacaaaagc atcgtccagc cgctgcatga cggtttccag
    21181 ctcatcgtct gacaggttcc agtcccgggc tatgtggcgc acatccccac gggtcagcac
    21241 cacggcaaaa acgcattcac tgtccggcac gtctgtatcc agtacatcgc ggatgatttc
    21301 tttaacagta cagtacatgt tctcgtctcc tttacagggg cggcattgcc gccccgtccg
    21361 ttaatcagtc cagcgccgcg taaatcgcgt tgcattcagg gtgaaattcg atgtggctcc
    21421 acaactgcgc atcgcgcatc ctgtacaggt gagtcagccc tgcatccccg ctgtcgtggt
    21481 acagccacaa ctggcggtta atcacaaggg aggtaaggat gatgcctgcg gcatcggcac
    21541 tgaccgtgcg gtcaaaccag tttgcaccgt tcaccatgtg gaaacggtca ccatccggca
    21601 tcatgtaacc gccaccgtcc ggcaggcgga tgaaatgcca gaagcctccc tgatattccg
    21661 gcatcatttt ttgcgccagc gcgtacacat acgcctcggc atacagaaaa tcgttgccaa
    21721 acaggtaagg cagaaaagaa aggcggtcgc actcttcaac gttcagagtg acagatttcg
    21781 catactgcat gggatttcct ccgttgttca catattccgc gacaccgggc ggtgccgtgc
    21841 ctccatcttt gccgggcctg cagggttgca agggcgcaag ggaagccgca gcctgacgca
    21901 cacggctgac gggagttgga gggtgagacc gcgaaatctg agcgggtgaa cgaccggaaa
    21961 atcacggcag acgggaagga gggaccgggt gaccgcagcg gttcacctta ccttgcaacc
    22021 cgcccgcaga agcccggcta cgatggaggc acggcacagc ccgcacacga cgctgaggct
    22081 acagagttga aaacccacaa ggccatgccc cgcatggccg ggtaaagcgg agcctgcggc
    22141 gacatccttt tgctctttcc ggggaccggc tggaaaaggc ggagattttt ccccgccagc
    22201 gtcatcattt ccggattttc gccagtgcat ccgccatgct gcggatttca tcccgtgcct
    22261 ccagaacaac ctcccgcaga atatcccggt caaaccagcg acgggacggt tcatccggta
    22321 tacgcagcac atcacagacc agaccactgg ccaggatatg ctccacacac tcactgacca
    22381 ggtacatttc cacagaagaa aaacgggcgc cacggttcag aatggcatcg agcaccacgg
    22441 gcagattttc gtcatcttca tcaagccagc aggggtaatc gctttccttc acgaccagac
    22501 gaaagcggcg cgtctggcgg gtcagcaacg tctgaccggt ggtggtcagc gccgcccgga
    22561 tacgccgcgc atcgtctgcc acctggtaac agatgcttcg tactgcattg cgcaccgcat
    22621 caagaactgc cctgatttct tcctccgtca gccactcacc ggcatacggg aaagacacct
    22681 gcgttccgtc tgccggaagt accggcagcg cctgccactg gctgccggac agaacaagtt
    22741 ccgctccggt tccggtcagt ttctgaatgg cggaggccag gtgcggcggc agcacgccat
    22801 cgtggcggtt aacgagtaac gtcgccagcc ggggcgcatc cagccgcacg taaatgctga
    22861 tccagcccgg atagttcgga tttcgctggt caacatccgt ctcttccggc cagccctgcg
    22921 ccagcagcag gttacgggcg tggcgacaaa tcagtgcatt cagcgttttg ttcatgatta
    22981 atggtctccc tgaatcgccg cccgcaggcg gcgataacat cagtcgtttt cgcgtgtgtt
    23041 gtttttcagc agggcgcggg cctgattaac caccgcctgt cggcgggtga cgcattccgg
    23101 gacggcgagg ccgttatcac gggcggccca ctcacgtttc cacagtgcct gttcagcggt
    23161 ctgaatcagg gacgccagca gttcagcagg aacggccacc tggcggtttt cgctcacgtt
    23221 gctgataatc tccatcaccg cggcggaaga aataccggat tcagtcctct ggtcttccgg
    23281 gatgtcctcc aggcgcgcca ggacggttct gatttcatga tcggacaggg aaatatccat
    23341 tccgtcagcg agggcctgaa tttcttccgg ggaccagata accagcatca gcggcgtgtc
    23401 tcccggatac tttgctgcca gctcccggca tagcgtttcg catgttccgt acattctcgt
    23461 ctccttctca atctttaaag cacgcatcca gttccagaat ttcggtatat gcctccatca
    23521 ggaggcggtt tgcgcgaaac agcgtctgac gcaccagacg ttccggcagg gtgtcggcat
    23581 taaagctgta ccgctgctcc atctcattca tggttgtacg caggtcgcac atggcattgg
    23641 ccacctccgc cacttcggcg cgcagttttt cgtgtccggc atagttcatt tacgcagcct
    23701 ccggcataaa ccagtcatca ttcgcggccc cctgctgcat ggcccgttgc acggcggcca
    23761 gtcgttgctg tccggcacgg tgatactgtt caagcagctc gataccgata taccggcgtc
    23821 cggactgaag ggcggccacg caggttgagc cgctgcctgc aaacgggtcc agcacaatcg
    23881 cgttcggatg tgtgaagctc tcaatcagtg gttgcaggct ggtaacgggc ttttccgtcg
    23941 ggtgatgacg attgcctgaa tatttccagc ccagcacatc cggcagaggg ttacggggcc
    24001 gggccggact gccctttgcc aggatgtaag cgcattcgtg gcggtacgcg acatacgcag
    24061 atttggatgt gtagtttttg gtgaacacga ggtgccctgc cacgctgaat ccggcgcgtt
    24121 tccaggccgc cataaagcgg tcaatacggt gccagccgta gaagctgacc atcagcgcgt
    24181 cttttttgag tacgcggtac atttcattac aggccggttg cagccattca tcggttttat
    24241 cgccagcgat ggtgcgcccg aaacggtcac gaaaaccgac gaggtacggc gggtcagtca
    24301 gtatgaaatc aatggcgtta tcaggaatac gggccataac atcgatgcag ttaccgagga
    24361 caaaacggga cataacaaca ctccttctct tacagaaggc accaagaggg cacccctgtg
    24421 gtgcccgttc cccgctgcct gtgggctgtt caccgcgtcc ggtaacgggt caggccgacc
    24481 gtggaatacc gcagccgtcg tctttttgac ggcaaggcta tgccgcgaaa gcggcttgaa
    24541 gggcgttcat cagaaaaggg cgacgggctg gcgtacactg tcaccgacgc cagccgggag
    24601 cggtaaaagc gcccgtcccg tgcccggccg gacgcgggac actgaccaca ggcagcgttt
    24661 atgccgcgcc gcgtcagccg gcgcagtaaa aagaggagca cggcgacatc ctttacagga
    24721 aaagaaacgg gcggtagccc gcccgtctcc gtcatacttc atccgccata atgcgccacc
    24781 ttgtcgacta tccacgcagg tccggtgact tccggcagct catcgtcatc tgtcggggaa
    24841 gaccattcca gctcccccca cagcacatca acgagctctc cgcgctcata caactgccag
    24901 ccacagaaat cacagccctg ttcggcatac cagtgctcca gcgtgcagct gaaacggcgg
    24961 cttaattctg caataacgtc gctttccggc tgacaccacg gcgtgtcgaa atcaacctga
    25021 atgaagttgt ctccctgact gctgatattc aggtcatacc cacaaggcca cttcacgcca
    25081 tactgttcgg tataccagtg atatgcagaa ggaacaccgt tcagaacacc accgttaaag
    25141 ccattcactt caacatcaag gcgggtgggc agaaccgtca gcaggtcaaa cggcatggtt
    25201 ttttccggca gtacgttgtc acacagacgg ttccaccaca ccgatacatc ctcgttactc
    25261 cagaagccac accagtcacc tctttttgcg gtgaaaagcg cggtgatggt atccctgaca
    25321 tcgtccggta atccttccca gcgacgctgg ccagtaccgc tctgcagcca gagttcatgc
    25381 agcatcaggc agttttgttc atccagcagt acaccgtcct gcaggtgtgt cagccagcgg
    25441 gtgaacgcga tattctccgg cgatacaacg ccacgtccgg cagccgtcag tccggggcac
    25501 ggttcaaaca acacatcttc agtggtctgc agaagtccgg cacttccggc cagaaacagc
    25561 tgaatacctt catttgtggc gcggcgataa agcggtgtga cggcaccgct ggccagtcgt
    25621 ttaatctcag caatctgtgc cggttcacca gaaaaatgca tacgatttga gcaccagttt
    25681 ggcatgggaa ttctcctctt acatgcggcc tccatcaggc cgtgccgcac ctgtaaccgg
    25741 gcgaccgcat acgaatgcag gtgcggcccc gaagccgcag ctcgtggagg aggaacgacg
    25801 aaacgtgacc gggtgaggag gccggcgaca gcgacacact tgcatctgat gcagcccggt
    25861 taacgtgccg gcacggcctg ggtaaccagg tattttgtcc acagaaccgt gcacaaaatg
    25921 ctgtggataa gctggacaca gcacttcaca gcaggcatac agccacaccc tcagagaatt
    25981 gagcgttttt taatcagaac aatgacattt gactgtgtgc ggattcagtt attagaatac
    26041 tgtatatgca tacagcaaaa gaaaaggaag atgagaacat gatccgcatt gaaattcttt
    26101 tcgaccgcca gagcacaaaa aacctcaaat caggcacact tcaggcgcta cagaatgaaa
    26161 tcgaacaacg tctgaaacca cactacccgg aaatctggct gcgtatagac cagggcagcg
    26221 ccccttctgt atctgtcaca ggagcccgca acgacaagga taaagaacgt atcctgtctc
    26281 tgctggaaga aatctggcag gacgacagct ggctgcctgc agcatgagta ccgtttatca
    26341 ccgtccggct gacccttcag gcgatgacag ttacgtgcga ccgttgtttg ccgatcgttg
    26401 ccaggccggt tttccttcgc ctgccactga ttatgctgag caggaactgg atctgaacag
    26461 ctattgcatc agcagacctg cagccacctt ctttctgcgc gccagcggtg aatcgatgaa
    26521 ccaggctggc gtgcagaatg gtgatctgct ggtagtagac aggggcgaaa aaccacaaca
    26581 cggggacatc gttatcgctg agatcgacgg tgagttcacc gtcaaacgac tgctgttgcg
    26641 cccacgcccg gcactggagc cggtttcaga cagcccggaa ttccgcacac tgtatccgga
    26701 aaacatctgt atttttggtg ttgtcactca cgtgatacac aggacgcggg agttacgctg
    26761 atgtttgcac tggctgatat caacagtttc tacgcctcat gtgaaaaagt tttccgcccg
    26821 gaccttcgca acgaaccggt catcgtactc agcaataacg atggctgtgt gatcgcgcgc
    26881 agcccggagg caaaagctct tggcatcaga atggggcagc cctggtttca ggtgagacag
    26941 atgcgcctgg agaagaaaat acatgtattt tccagcaatt atgcgctgta ccacagcatg
    27001 agccaacggg tcatggctgt tctggagtcg ctttctcccg cagttgagcc ctactcaatt
    27061 gatgaaatgt ttattgattt gcgggggata aatcattgca tctctccgga gttttttggt
    27121 catcagctca gggaacaggt aaagagctgg acaggactca ccatgggggt gggcattgcg
    27181 cctacaaaaa cgctggctaa aagtgcacag tgggcaacaa agcaatggcc acagttttcc
    27241 ggtgtggtcg cgctgacggc agaaaaccgt aatcggatct tgaagctact ggggctgcag
    27301 ccagttggtg aggtctgggg agtaggatgc agactgccgg aaaagctgaa tgcgctgggt
    27361 attaacacag cactgcagct ggcgcaggct aacacggcat tcatccggaa aaacttcagc
    27421 gtcattcttg agcgtacggt acgcgaactc aacggcgagt cctgcatatc cctggaagaa
    27481 gcaccaccgg caaaacagca gattgtctgt agtcgcagtt ttggtgaacg aatcacagac
    27541 aaagatgcca tgcaccaggc tgttgttcag tatgcagagc gggctgcaga gaaactacgt
    27601 ggggagcgtc agtattgcca gcaggtgacg acatttgtac ggacatcccc ctttgcagta
    27661 aaagaaccct gttacagcaa tgccgctgtg gaaaagcttc cattgcccac acaggacagc
    27721 cgggacatta ttgccgcagc atgcagagcg ttaaaccatg tctggcgtga agggtaccgc
    27781 tatatgaagg caggagtcat gctggctgat ttcacaccat cgggtatagc gcagccggga
    27841 ttatttgatg agatccagcc ccgtaaaaac agcgaaaagt taatgaaaac actcgatgaa
    27901 ctgaaccagt ctggaaaagg gaaagtgtgg tttgcggggc gaggaaccgc ccctgaatgg
    27961 caaatgaaac gggaaatgct ctcgccttcg tatacaacac aatggactga gatccccatt
    28021 gcttcattct gatatatcag aataattttt gcattttaag gcgcacatca ttcattgcct
    28081 cctgcaccga atcagtagca gaaggaggca ttttttcagc agattgcaaa gcaccgagtt
    28141 cagtcaacac agctctgata tcggcctgag aaggcttcca tccagtcgtc tgactgagta
    28201 aggttacaac ctgatctgct gaaaactcct cgctcaaaat ggcagtcagc aatacaggca
    28261 ggcgaggatc aagttggtgt aacgccattc cagaaataaa tgcattccgg aacagctcac
    28321 cacgctcaga acgcggaacg gactctatcg cttccaaagt ttgaaagtct gcagcttttt
    28381 ccggatgcag ataaagtgtg aattttttcc ttttccgttc atcgtccaca atattactcc
    28441 tctttgaaag ccgcgatagc ttcaaccagt gctgtctgtg gcgactccat catcactact
    28501 ttttgcccaa gatggtgcca tgccgttttt atcgaatcat agatcaacgg ggccccacca
    28561 ccgacaacat aaacacgatt cacatgatga aaatcaccga gctcattgac tacttgctcc
    28621 cccagactgg caatagcccc ctcaatggta ttaaggacca gatcagtttt tgtttcgtca
    28681 tttataacct gccgaacaaa gtccgggtca ttacgacgtt taatcaattc atctgcaacg
    28741 aggaaactgg tatcactgga tgccatgcgc aatgcactca ttgcagcttt cgtaactgaa
    28801 gacacaccaa tgccagaatt accatgaatg gcacttacag agtcaaattg accaacgata
    28861 acaccaacat caagcgtagt gccgccaata tcaactacca gcgatttttc gaactgacca
    28921 actttatcca tgaccagacg agaaaacaca gctggcaatg attcaggcat cacatcaacg
    28981 tgttcaatgg tgaaaacatc gcctttatta agacgaatcg gcctcattaa attctcaatt
    29041 ttacgctgaa tgttgagttc atttttttga cactctttcg tgtagaactc actgataggc
    29101 aaagtcacag tcaatgatac aggctgaggc actaatccac tattcagtaa agcgtgatgc
    29161 acagctaaca ggttaacatc cgtatattga tactcaatat gagtcgtaag aatagattgg
    29221 ttgctaactt catcatacgt gtatttttta ccatccagtt catagttaaa tgtctggcga
    29281 atccctaacc cgtcaacctt ccatccatga cgaaatgagt tttgcgacaa cgacttactg
    29341 attttcccat tgtcattcca tgccaacttg attgttgttg aaccatcatc gcagtatacg
    29401 ttcatctata gccccttaaa tactcaatct gagtaaatca ctgtttctgg ttgcaaggta
    29461 atactcaatt tgagtactat catagttatg aaatcacaaa acacaagtat tatttattac
    29521 atcatatgac acacttcatg tttcatactt cataaatatg aacacatatt gacacctcag
    29581 gttaccgtgt caaaatatca tcatacttca cacatcacat ttatgaggtt gcaatgatta
    29641 ctgtagttgg tgggaacaaa ggtggttcag ggaaaactac cattgcatca aacctggcta
    29701 ttgctctcgc aaataaagga agggaagtct gcctgttaaa cggagatcta caacgaacag
    29761 ccgcaaaaca tcatgcggaa cgagaagctg caggactttt accagcaata actcttgttg
    29821 agaaatttga taatctgaca caaacactac aggctctgaa tgaaaagttc gatgatgtaa
    29881 tagtggatgt cgcaggaaga aactcaaagg aattcataac gagtggagtt gtcgcacatc
    29941 aaataattgc gccactacag tgttctcaac cagatctgga tactcttaca gaactagaac
    30001 aacagataga cgcaatgcgt aaccttaacc cggaacttaa ggtttattgt ttacaaagta
    30061 tggcaacaac caatccggtt ttgaggggaa atgagagaaa agaattcctt gaatatcttg
    30121 aagaattccc aacaattcaa gttctggatt cagttatttg tttcagaaaa gtctatcgcg
    30181 attgcatgag caacggcacc ggtgtagtag aaacgaacaa tacagcagcc aaagcagaaa
    30241 ttgaacattt aatgaatgag gtatttggcc catggtgaag aaacccagcc agcaagcatt
    30301 aaacagagct gcagttacgg tagaacaagc agaggctctg gctcagcgcc ttgcggataa
    30361 accatacggc gcgccggaaa aacccgaacc agaaaaacaa tgtcggacga caatctctct
    30421 tggtgaaagc atgcttgtca caatagaaga tctggcattg aggaataaac gcaatggtaa
    30481 agatccaaaa aatgtaagtg ccatcgtcag agtagccctt gagcaatact taaaaacact
    30541 aacataataa tcaaaaagtt attacttgtg aaatcacact tgtgaattca tactgtttaa
    30601 gcccccttgc atacaagaga tcacatattg cagtggtctc tttttttaca gaataaatca
    30661 caattcataa ttatgattta tgaattatgc gtgtctgttt ttcagcattg tgaccgcatc
    30721 ggactcaggc atcgaaaact gcacccggtg ccgggcagcc acatccagcg caaacacctt
    30781 cgtgtagacc tccgttgagc tgatggactt atgccccatc aggctctgca ggaccttcag
    30841 cggtataccg ggatacagca tgtgcatcgt ataggaatgg cggaacgtat gtggcgtgac
    30901 cgggacagag aacgtcacac catcagcggc agcggcggca accgcctccc caatccaggt
    30961 cctgaccgtt ctgtccgtca cttcccagat ccgcgctttc tctgtcctgc ctgttcgttt
    31021 attacgccgt tccatgggga ttttcagtgt ggccactatc gtctgcagct ggctgacgta
    31081 ccaggagtcc gagagcggaa ccagccggtg agtctgctgg ccggcgggca ttcttcctgc
    31141 cgtcctgacc agtaatgctt gtgcagtttt ttgtggtatc aggcgagaat agcacgtggt
    31201 gtgagtgcct gttttttgat cgttttcaca aaaattgaaa tccacagtct tgacagagaa
    31261 aacacagcgg cgtaattttt tatctgtaca taaaaccagt ggttatatgt acagtattta
    31321 actttaattg attgttttta aagtcaaaga agttttctat gagtgataat gaaggtagtg
    31381 taccgacaga aggtattgat tacggggaca caatggttgt gtggccgtca acaggacgaa
    31441 ttccgggcgg tgatgtgaaa cccggaggct catcaggtct cgctccatcc atgcctccgg
    31501 ggtgggggga ttacagccca caaggtatcg cacttgtaca aagtgttctt tttcctggaa
    31561 ttattcgccg gattattctt gataaagaac ttgaagaggg agactggtcg ggatggtctg
    31621 tcagtgtgca tagcccctgg ggaaacgaga aagtttccgc tgcacgaaca gttcttgaaa
    31681 atggtttacg tggtggtttg ccagaaccgt ctcgcccggc tgctgtttct tttgcccgtc
    31741 tggagcctgc ttccggaaat gagcaaaaaa ttattcgtct tatggttaca cagcaactgg
    31801 agcaggtaac ggatatccct gccagccagt taccagcagc gggtaataat gtaccggtaa
    31861 aatatcgtct gacggacctt atgcagaatg gtacacaata tatggctatt atcggaggta
    31921 ttccgatgac agtgccagta gtggatgccg ttccagttcc ggaccggagt cgtccgggaa
    31981 ccaatattaa agatgtttac agtgcccctg tatcaccaaa tctaccggac ctggtattaa
    32041 gtgtgggtca gatgaatact ccagttcggt ctaatcccga aatccaggaa gatggcgtta
    32101 cttctgagac agggaattat gttgaggctg gttatacgat gtccagtaat aatcatgatg
    32161 tcattgtccg ttttcctgaa ggcagtggag tttctccgct atatatttca gccgtggaga
    32221 ttctggacag taatagttta agccagcgcc aggaagccga aaataacgca aaggatgact
    32281 tcagagtcaa gaaagaacaa gaaaatgacg agaagacggt cctgacaaaa accagcgagg
    32341 tcatcattag tgtcggtgac aaagtcgggg aatatcttgg agataaatac aaggcgcttt
    32401 cccgtgaaat tgcagagaat ataaataatt ttcagggaaa aacgattcgt agttatgatg
    32461 atgcaatgtc ttccattaat aagttaatgg ctaaccccag ccttaaaata aatgcaacgg
    32521 acaaagaagc cattgtgaat gcgtggaaag catttaatgc tgaggatatg gggaataaat
    32581 ttgctgcgtt gggtaaaacg ttcaaagcag cagattatgc aataaaggca aacaacatca
    32641 gggagaagag tattgagggt taccagactg gtaactgggg gccattaatg ctggaagtcg
    32701 agtcctgggt tatcagtggg atggcatctg ctgtagctct tagtttgttt tctttgacat
    32761 taggctcggc ccttatagcc tttggtcttt cgtccacagt tgttggtttt gttggcgtag
    32821 ttattgcagg tgctattggt gcatttatcg atgataaatt tgttgatgag ttgaatcaca
    32881 agatcataaa ataattaaaa ggaaggcagt gttactgcct ccctttaaaa tatttaatga
    32941 caagcaatgg catcagtaaa atcgcatatg tcatggttaa aacagtaaag taaatggaac
    33001 aatataaaat aaacagagtg atgacattat gagataataa tttaaaaaaa ccacctgctg
    33061 ttgttagttc cagattatag aacagtgtga tataaatcag aatggcataa aaaacgggcc
    33121 cccagaaaca ggattgcaca acaagttttc ttgttactgg ctttatgatt tctcttgtct
    33181 tatatgtgca aagaaaaacg agaataccca taactggtgc tgtcttgttg tagtttgaca
    33241 taagtgttgt catgacggga tcatgcaatg atgtaataga agggaggaat gccaccttat
    33301 tatataagta gtacagcact tgagaatatg gattatttat tcgaagtatt aatatagcca
    33361 ttaatggaat aatcccgatt atggaaaatg catataatat ttcgtttgct ttcttgttct
    33421 tatcttt
//
LOCUS       DAZFYC010000043        33218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFYC010000043 DAZFYC010000000
VERSION     DAZFYC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.043"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..762
                     /locus_tag="ACHKW5_002301"
     CDS             <1..762
                     /locus_tag="ACHKW5_002301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511688.1"
                     /translation="NANDNHLIYSNLNDTDINSKIFDAVNGEISAKKLADELFPENEP
                     HIFISHSSKDVNVAISLANTLYAKYKITSFIDSQLWGHIDHALKEMHNRYCKIPGSSY
                     YHYDKSNNLLSHMHAILSMALMRVMDNADSVIFIESGNSIYQYMGDTTIAPQEAIEKT
                     SETLSPWISSEVNFANTLRLKGHKDREQPLMESTDSYSLNKSNIKADSLPKIIHEVNL
                     SKFIEIDNKKFKESLSLNETNPIKTLDMIYQIYAQ"
     gene            complement(914..1000)
                     /locus_tag="ACHKW5_002302"
                     /pseudo
     CDS             complement(914..1000)
                     /locus_tag="ACHKW5_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1006..1110)
                     /locus_tag="ACHKW5_002303"
                     /pseudo
     CDS             complement(1006..1110)
                     /locus_tag="ACHKW5_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1140..1658
                     /locus_tag="ACHKW5_002304"
     CDS             1140..1658
                     /locus_tag="ACHKW5_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7511689.1"
                     /translation="MPFARYFCIFINVGLGEGSALPVGVPVPWPSATPPTGWLKCNGA
                     AFDKVKYPHLATAYPSGKLPDLRGEFIRGWDDGRGIDAGRALLSIQTGMLEKHRHIVV
                     ANDGYDSKEEWELATIFRRAYTQGRGLDAADAGGTLIPSPTLHTRGSIGNTGGSETRP
                     RNIAFNYIVRAA"
     gene            1658..2260
                     /gene="tfaE"
                     /locus_tag="ACHKW5_002305"
     CDS             1658..2260
                     /gene="tfaE"
                     /locus_tag="ACHKW5_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7511690.1"
                     /translation="MDKAVLNSELIATKAGNITVYNYDGETREYISTSNEYLAVGVGI
                     PAYSCLDAPGTYKAGYAICRSVDLNSWEYMPDHRGEIIYSTETGEAKEITAPGDYPEN
                     TTTIVPLTTYDKWDGEKWVTDSEAQHGAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(2232..2672)
                     /locus_tag="ACHKW5_002306"
     CDS             complement(2232..2672)
                     /locus_tag="ACHKW5_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7511691.1"
                     /translation="MNYIYSATTNSFYPLEMKEDYTQADSWPDDAVEVDEQVYIEFSG
                     LPPEGKIRIAGENGFPAWSEIPPPTPEEQIAAAELEKQQLINQANDYMNSKQWPGKAA
                     IGRLKGEELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(2694..3383)
                     /locus_tag="ACHKW5_002307"
     CDS             complement(2694..3383)
                     /locus_tag="ACHKW5_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511692.1"
                     /translation="MAKNDFKAFATDRNANVISQEEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALENLGLGEAAKRNVGTGANQIPDMGSFTLSVSGTGYQKLPSGFILQWGSIG
                     APGIAQDVVTHFPIAFPNRCLRVLVSQDYTPDSGAVGYIACAGFSPDPVKFISRTSTP
                     GLGASFLALGC"
     gene            complement(3383..4063)
                     /locus_tag="ACHKW5_002308"
     CDS             complement(3383..4063)
                     /locus_tag="ACHKW5_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFO7511693.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGDGLRMQIVDNQDMTISVWVFPDT
                     DISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGVLL"
     gene            complement(4060..5259)
                     /locus_tag="ACHKW5_002309"
     CDS             complement(4060..5259)
                     /locus_tag="ACHKW5_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7511694.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNNVIWRLPASVVVGVDGTVMVTATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGTPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVTGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQAISDYINSLMIG
                     DSVLLSRIYSPANLGVVSSGNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            complement(5259..5612)
                     /locus_tag="ACHKW5_002310"
     CDS             complement(5259..5612)
                     /locus_tag="ACHKW5_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511695.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVRFST
                     EIDTLYGITTVTSEA"
     gene            complement(5612..6364)
                     /locus_tag="ACHKW5_002311"
     CDS             complement(5612..6364)
                     /locus_tag="ACHKW5_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp138 family membrane-puncturing spike protein"
                     /protein_id="HFO7511696.1"
                     /translation="MAVSDQTRSGDLAETFKSERETTKNQIRVALPGIVQSFDPDAVT
                     AVVQPAIRSVETDNDGNRVTKNYPLLVDVPVVFPRGGGCTLTFPVKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            complement(6426..6821)
                     /locus_tag="ACHKW5_002312"
     CDS             complement(6426..6821)
                     /locus_tag="ACHKW5_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511697.1"
                     /translation="MSVTSNQILDTARLCLSENIESGFRSAISRAYYSMLHESISSLT
                     AIPHFTHEHHKNTVGYMSTPSECKSEPYPTNTLKSLAFVLRQWRDARNEADYDLTNVT
                     VSKEMGQDAIEAAELYFERWEQLKSARAS"
     gene            complement(6808..7581)
                     /locus_tag="ACHKW5_002313"
     CDS             complement(6808..7581)
                     /locus_tag="ACHKW5_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511698.1"
                     /translation="MAAGIPLEKAQKYVEQFNALLLDRERLDPFTEKKIREELSVSPT
                     PATNIALSYLDAITGKIESALRYLRISLEVNDVTLAMHYHHILINTFSYNELKDVYVP
                     LAKKYRTKLFSHNAYSWAYRCGEREQLEFYMEEHIKLLSESEGRSNAMKHKEELLAEM
                     DNFYSATQCSKEQFQTLASIIWGLLSEYKATTGFVQLSGSGCYVVDIKNLEPKTIAKM
                     NFDLADRVCAESKLDDCSLLARFTSPRQLHTGVSYHVGN"
     gene            complement(7677..8024)
                     /locus_tag="ACHKW5_002314"
     CDS             complement(7677..8024)
                     /locus_tag="ACHKW5_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511699.1"
                     /translation="MKRMKLVLTISLLFSCSSAFADLQCGGYRLHASDNGWTKINGEQ
                     VTSQKIKFLGQKDDWDNVKTDMGLMPSRDGNNYGFEFVKRNGKAFLNVQLLQNSMDAP
                     RIIGSFPCKKALR"
     gene            complement(8021..9088)
                     /locus_tag="ACHKW5_002315"
     CDS             complement(8021..9088)
                     /locus_tag="ACHKW5_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511700.1"
                     /translation="MSQNWMRHFELQLVDGNGQGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVSASDVGTVREVDAADVGQSDGR
                     NYGMIFSGEIRYSVTGKDSPIDSYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVFPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     NNEYVHEAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     SNDIAMAGGQITDQNTDGNITLSGTTSQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAADLLSSSAQQRFYS"
     gene            complement(9091..9393)
                     /locus_tag="ACHKW5_002316"
     CDS             complement(9091..9393)
                     /locus_tag="ACHKW5_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511701.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGATWRISIIWRDPYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLLVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            complement(9393..9980)
                     /locus_tag="ACHKW5_002317"
     CDS             complement(9393..9980)
                     /locus_tag="ACHKW5_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO7511702.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLDLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVLISRTQQITVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            complement(9980..11968)
                     /locus_tag="ACHKW5_002318"
     CDS             complement(9980..11968)
                     /locus_tag="ACHKW5_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HFO7511703.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYWASQRTGATVQGIQSIGYAVSQVGGSVDAARSSLESL
                     SRFIRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSSMPYYRANQYAQMLG
                     IDENTLMAMRRGVGQFSAQYSEMVKAIGFNADEAARSSNKFMTSLREFSAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILALGDIIGRLFFRLIEGTSG
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKTTVNDLVKALAKLLNIDLKSWSLKWDFSKF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVSIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQHAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMRDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKMTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
     gene            complement(11958..12134)
                     /locus_tag="ACHKW5_002319"
     CDS             complement(11958..12134)
                     /locus_tag="ACHKW5_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP pyrophosphohydrolase"
                     /protein_id="HFO7511704.1"
                     /translation="MPDGLSYLLNPVDAGLIPYTALKDGSVDLYDIALLNDHLAVKAD
                     NQRRIEKWREDNER"
     gene            complement(12146..12598)
                     /locus_tag="ACHKW5_002320"
     CDS             complement(12146..12598)
                     /locus_tag="ACHKW5_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO7511705.1"
                     /translation="MEFEIKGVKYRTAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKFEQLKPVFETMLPRIAEELSSLTEDDTDAIIHPCLAVVSRRHMDRWV
                     PVFTQGELMFDDIDLLVMLQLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            complement(12602..13042)
                     /locus_tag="ACHKW5_002321"
     CDS             complement(12602..13042)
                     /locus_tag="ACHKW5_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HFO7511706.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGADGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            complement(13053..14198)
                     /locus_tag="ACHKW5_002322"
     CDS             complement(13053..14198)
                     /locus_tag="ACHKW5_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HFO7511707.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKTQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPNADWLKVAAAIESSSVSRILAITTSDDKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASVLDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSNVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            complement(14202..14765)
                     /locus_tag="ACHKW5_002323"
     CDS             complement(14202..14765)
                     /locus_tag="ACHKW5_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511708.1"
                     /translation="MGELRLSNNTSTELGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPAETNWCAFGVIEWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(14740..15129)
                     /locus_tag="ACHKW5_002324"
     CDS             complement(14740..15129)
                     /locus_tag="ACHKW5_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HFO7511709.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(15116..15670)
                     /locus_tag="ACHKW5_002325"
     CDS             complement(15116..15670)
                     /locus_tag="ACHKW5_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511710.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKTAAQAALDGNAAGAESA
                     LNRAGTLAVNGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRAAGESPGTELA
                     KPLIDTGQYRRAITHIVRDKDADS"
     gene            complement(15667..16074)
                     /locus_tag="ACHKW5_002326"
     CDS             complement(15667..16074)
                     /locus_tag="ACHKW5_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HFO7511711.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGSGGSTNGVQASKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            16073..16306
                     /locus_tag="ACHKW5_002327"
     CDS             16073..16306
                     /locus_tag="ACHKW5_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511712.1"
                     /translation="MFYLVTRVCGFANGYFRVRTSEPVRGNVFLHSMTAFNKLAPRTV
                     GKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(16303..17244)
                     /locus_tag="ACHKW5_002328"
     CDS             complement(16303..17244)
                     /locus_tag="ACHKW5_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFO7511713.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDTGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDCVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGKKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(17256..17762)
                     /locus_tag="ACHKW5_002329"
     CDS             complement(17256..17762)
                     /locus_tag="ACHKW5_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511714.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
     gene            complement(17766..18986)
                     /locus_tag="ACHKW5_002330"
     CDS             complement(17766..18986)
                     /locus_tag="ACHKW5_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFO7511715.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWSRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEQETTITGDSAWRAEVIVPGIDLSRKMKPTEFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(19001..19735)
                     /locus_tag="ACHKW5_002331"
     CDS             complement(19001..19735)
                     /locus_tag="ACHKW5_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO7511716.1"
                     /translation="MTNRRNRQAEIRYRTSLRRIARAVGDIVNGHYDGSNDSITEIIE
                     ALERYSEIITPWATKVAENFTADIVRKNDEQWRKHSKTISRELRNLVNSSPPGQVMKS
                     IVAEQVKYIKSLPLEAADRVYDIQNRAIEAVVTGGRAEHFAKEIAASGDIAKSRADLI
                     ARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDGM
                     TGHAGELPNCRCYKEIVFPSSHSYPA"
     gene            complement(19626..21092)
                     /locus_tag="ACHKW5_002332"
     CDS             complement(19626..21092)
                     /locus_tag="ACHKW5_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFO7511717.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYKVEKLRELIALGGQAFEALLKNIDLIRQFQ
                     SNEGMTLMDAKDTFETHQYNFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSAGGRGHRKWSLRWFK"
     gene            complement(21092..22714)
                     /locus_tag="ACHKW5_002333"
     CDS             complement(21092..22714)
                     /locus_tag="ACHKW5_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HFO7511718.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPVLKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWSEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(22717..23289)
                     /locus_tag="ACHKW5_002334"
     CDS             complement(22717..23289)
                     /locus_tag="ACHKW5_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7511719.1"
                     /translation="MTKKLKAKHEVFCREFLVDLNATQAAIRAGYVSRRAHVTGAELY
                     GKPEIRARINELKQERIDQLGIDANYVLMRLVEIDRLDVADILEDDLSIKPLSAWPES
                     WRRYLSGFNLAEMFEGRGDDREMVGILKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(23351..23875)
                     /locus_tag="ACHKW5_002335"
     CDS             complement(23351..23875)
                     /locus_tag="ACHKW5_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511720.1"
                     /translation="MKLTYKIVIAAFFFSAFGALVWSANHYHSKYQAEKLRADKAEGE
                     AEYQGKVIANQALNFNRFNQIAENASRLNSLVDIGHEKTVIKYREVLLREKNCDFPVP
                     VDIAVGLLNYANRLRASALHADSGDIDSAGDRATTTRTLTYCQAVLWINPLLAAIEKA
                     NNQLAGVRQIEQSR"
     gene            complement(23859..24335)
                     /locus_tag="ACHKW5_002336"
     CDS             complement(23859..24335)
                     /locus_tag="ACHKW5_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7511721.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFNDAGGVVNEADL"
     gene            complement(24339..24680)
                     /locus_tag="ACHKW5_002337"
     CDS             complement(24339..24680)
                     /locus_tag="ACHKW5_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO7511722.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIVMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGGSKE"
     gene            complement(25126..25467)
                     /locus_tag="ACHKW5_002338"
     CDS             complement(25126..25467)
                     /locus_tag="ACHKW5_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511723.1"
                     /translation="MALISVRNRFESFMEQRYPDLSLQVKGNISASMKAQLGIRIERE
                     LYSEDVTYCDSAVQLMWTLFQAGVLAERRATSVTLPALKAKPDSFYDAGYNEGIQDCR
                     KHLTASGIKVR"
     gene            complement(25499..25921)
                     /locus_tag="ACHKW5_002339"
     CDS             complement(25499..25921)
                     /locus_tag="ACHKW5_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7511724.1"
                     /translation="MRNIQQVLERWGGWAASEGGSVYFPPVAAGFKNLLPATQSGRLK
                     CSDNDGLIINSAMSCLKKKDPYLCTLLEWHYVQAMPVRAMGEKLGVSHTHVLKRLQAA
                     EGFIDGCLAMLDVVLEMDQSVQSKPQAIRTLRRSCSAA"
     gene            complement(26203..28395)
                     /locus_tag="ACHKW5_002340"
     CDS             complement(26203..28395)
                     /locus_tag="ACHKW5_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFO7511725.1"
                     /translation="MSDSKPWGATPDEWFHFDLVLGRTDHLLPVVCNPGATISPDSKL
                     KALGKTPSRYNRDRQVTGIAQWTGHVVTEHDFARWSNEPDYGICVRTGHGWLALDCDS
                     EDEDIQADIRKTLVQLLGELPPRRWRANSNKCLYLLAVDGDFRKRIHRLAGDMGIIEL
                     LANGQQFVACGTHSSGARIEWDGGLPDEPPAITGEQLETLWQRLAEQLPVSVTTEAGN
                     TKMRNRSAFTPGATDDTAEYLDANGWTLLDGANGERYIRCPFEDGHSSGGDPTSTVYF
                     PAGTAGFEQGHFKCLHASCAHRDDGDFLNAIGIRNDDFEDLTSTEVAEPLPLPAFERD
                     KWGRIEATISNAAKAVVRPDFVDIDIRFDLFRDEIMFAPAGSGQWRAFTDADYARLRI
                     TMEKRGFKPVGRELIRDVVLLAADEQPFDSAIAWLNGLEWDGVPRIECFYHTHFGTAD
                     TPYTRAVSMYMWTALAGRVLEPGIKADMVPILVGPQGCGKSSGVEALSPDPAFFTEIS
                     FAEKDDDLARKMRGRLVAEIGELRGLNTKELESIKAFVTRTHENWIPKYREFATQFPR
                     RLVFVGTTNEDEFLADKTGNRRWLPVEVSKVDVKAIKRDLLLLWAEAREVFQRLGGIQ
                     FREAEQLAASVHEQYTIKDAWLETVEKWLDTPDLMTNELPRNCEFLRASDVLRDAIGL
                     NPDRIGKREEMRISNVLQNCGYKRAQRRIGGKMTRIFEAVSQPVPASK"
     gene            complement(28399..28611)
                     /locus_tag="ACHKW5_002341"
     CDS             complement(28399..28611)
                     /locus_tag="ACHKW5_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7511726.1"
                     /translation="MTLATRVKERRKELKMTQVTLAELTGVSQQAINRIESGVIARPR
                     YLLEMSIALDCDPNWLLYGSQNDKKA"
     gene            28732..29355
                     /locus_tag="ACHKW5_002342"
     CDS             28732..29355
                     /locus_tag="ACHKW5_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7511727.1"
                     /translation="MSLAARFKARRLELGMTQVEVANSAGVSQQSIESIESGRTRKPR
                     NLLDLAKALKCSPDWLLNGKNIMPLAEISTRRIPILSYVQAGELTEARDITDLTGEFE
                     YILADSDIPETCFALRIDGDSMQPEFKEGDIVIIDPDLCPAPGEFVVAKNDGHEATFK
                     KYRPLGIGIDDFELIPLNPDYPIFRSADMNLQIIGVMIEHRIYRRKR"
     gene            29989..30138
                     /locus_tag="ACHKW5_002343"
     CDS             29989..30138
                     /locus_tag="ACHKW5_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511728.1"
                     /translation="MKPEHLYRLTGRDVLRYRRKNFDLMTGLAVATALGLIITFILLV
                     ARTTV"
     gene            30135..31037
                     /locus_tag="ACHKW5_002344"
     CDS             30135..31037
                     /locus_tag="ACHKW5_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511729.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATETENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRARALLKPALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            31040..32341
                     /locus_tag="ACHKW5_002345"
     CDS             31040..32341
                     /locus_tag="ACHKW5_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFO7511730.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVEAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERAQEAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWIDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            32357..32905
                     /locus_tag="ACHKW5_002346"
     CDS             32357..32905
                     /locus_tag="ACHKW5_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFO7511731.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWEPIYNQLEKKLNLCLHDGDEKAEYEGFPGNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(32958..>33218)
                     /locus_tag="ACHKW5_002347"
     CDS             complement(32958..>33218)
                     /locus_tag="ACHKW5_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511732.1"
                     /translation="FINVNFSGFLFIISRLFSFLCFVLFILFSIICIISIGQNEGLLN
                     YLVYMVMTIVLETVGFWMYDTFPSKKKICELNKELRKIKVPE"
BASE COUNT         8378 a         8894 c         7679 g         8267 t
ORIGIN      
        1 aacgctaatg acaaccattt aatttactca aacctaaatg acacagatat aaactcaaaa
       61 atatttgacg ctgtaaatgg tgaaatatca gcaaagaaac tagccgacga actatttcct
      121 gaaaacgaac cacacatttt tatatctcat agtagcaaag acgtaaatgt agcaataagt
      181 ctagcaaaca ctctttatgc aaaatataag ataacatctt tcattgactc ccaactttgg
      241 ggacatattg accacgcact aaaggaaatg cataatcgat actgcaagat ccccggctca
      301 tcttattacc actatgataa atctaataac ctactatctc atatgcacgc catactaagt
      361 atggctttaa tgagagtaat ggataacgct gacagcgtta tatttatcga atccggcaac
      421 tcaatttacc aatatatggg agatactacc attgctcccc aagaagcaat tgaaaaaaca
      481 tctgaaacat tatctccatg gatatcctct gaggtgaatt ttgcaaatac cttaagattg
      541 aaaggccata aagatagaga acaaccatta atggaaagca cagattcata ctctttaaac
      601 aagagtaata ttaaagcaga ctccttaccc aaaataatac atgaagtcaa cttaagtaaa
      661 ttcatagaga tagacaacaa aaaattcaaa gagtctttat cattaaatga aacaaatcca
      721 ataaaaactt tagatatgat ttaccaaata tatgctcaat aggtagtttt tatttttagc
      781 atctaataaa ccgttggcac atcatataca acgtgccaac ggaacgagtc aattacaatt
      841 attttatggc aggaaaaact gacgaatcaa tatctggttc ggttgctgta tcaatacggt
      901 tcagcaacac ctaatatttc ttccatgccg ccagcaatga ggtctcctct gccgccacaa
      961 tacccaaatc tacggcatcc tgaagcggtg caatatgctc accctgattt tatcttcaaa
     1021 aatccggtcg catcctgcac agttcaacgc attacgttgt agatcggtat tctggtcatt
     1081 tgttgatacg cgtacatagc caataagcat ggtagatctc cctgacaaaa gcaggaatga
     1141 tgccatttgc tcgttatttc tgcattttca taaacgttgg tttgggagaa ggctctgcat
     1201 tacctgttgg tgtgcctgtt ccgtggccct ccgcaacccc gccaacgggc tggctgaaat
     1261 gcaacggcgc tgcctttgat aaggtgaaat atccccatct tgctacagca tatccatcag
     1321 ggaaactacc tgatctccgt ggtgagttta ttcgtggatg ggatgacggg cgtggtattg
     1381 atgcaggacg tgctttattg agcattcaga ctgggatgct ggaaaaacac cgccatattg
     1441 ttgttgccaa cgatgggtat gattcaaaag aggaatggga actggcgaca atcttcagaa
     1501 gagcatatac gcaaggccgg gggcttgatg ctgccgatgc cggaggaact ctgattccat
     1561 caccaacgct acatacacga gggagtattg gtaacacagg tgggagcgaa acccgtccac
     1621 gaaatattgc atttaactat atcgtgagag ctgcataatg gataaagccg tattaaatag
     1681 cgaacttatt gccacgaagg cggggaatat taccgtctat aactatgatg gtgaaacacg
     1741 ggaatatatt tccacttcaa atgaatatct tgccgttggt gtcggtatcc cggcatattc
     1801 ctgtttagat gctcctggca catataaggc tggttatgca atctgccgtt cagtagattt
     1861 aaactcatgg gaatatatgc cagaccatcg cggtgaaatt atctatagca ccgaaacagg
     1921 agaagcaaaa gaaatcacag ctccgggtga ttaccctgaa aatacaacca ctatcgtccc
     1981 gttaacgaca tatgataaat gggatggtga gaaatgggtg acagattctg aggcacaaca
     2041 cggtgccgca gtagaagcgg cagaagcaca gcgccagtca ctgattgatg cagcaatggc
     2101 ttccattagt ctgattcagc tgaaattaca ggccgggcgg aaactgacgc aggcagaaac
     2161 aacccggctt aacgctgtgc tggattacat tgacgcggtg acggcaacag ataccagcac
     2221 cgcgccggat gtcatctggc ctgaactgcc ggaggcgtag gccattcaat atctggcgca
     2281 ctggaagtat cgaccagctc cagtgcgtcc agataatcca gccacagatt atattgcgcc
     2341 agttcctcac ctttcagacg accaatagcc gctttaccag gccattgttt actgttcata
     2401 taatcgttgg cctgattaat cagttgctgc ttttccagtt cggctgcagc aatctgttcc
     2461 tcaggtgttg gtggtggaat ttcagaccat gcaggaaaac cattttctcc agcgatacgg
     2521 atttttcctt ccggcggtaa tccggaaaac tcaatataca cctgctcatc aacttcaaca
     2581 gcatcatctg gccatgagtc agcttgagtg taatcctctt tcatttccag tggatagaaa
     2641 gagtttgtag tcgcggaata tatgtaattc atttttcact ccatatagct aaattaacag
     2701 cctaacgcta aaaatgaagc gccgaggcca ggagtactgg ttctggatat aaatttaacc
     2761 gggtcgggac taaaacctgc acaggcaata taaccaacag ccccgctatc tggtgtgtag
     2821 tcttgtgaga ccaaaacacg cagacatctg tttggaaatg caatcgggaa atgggttact
     2881 acatcctgtg caatgcctgg tgcgccgatt gagccccact gaagaataaa acctgatggt
     2941 aatttttgat atccagtacc tgaaacagaa agcgtgaagc tacccatatc aggtatctga
     3001 ttcgcccctg tccctacatt ccttttagcc gcttctccca aaccaaggtt ttcgagagcc
     3061 gttttcaccg tgccatccga tttgatatca ccaaacggat tcttgcggct caggtattca
     3121 acagcaaacc ccgatcccag caattcaaca aaaccgggca gatcaccatt atcaagcaca
     3181 tcccgttgcg ttttatcact tacaaactgg gccagagctg cagcaataaa gctggcctgc
     3241 cgaataacct tattgacttg cgcactggag gctttccctg ctgtaaatcc ggataaaagc
     3301 gcgggcaacg cttcccattc ctcctgcgat ataacattgg catttcgatc cgttgcaaac
     3361 gctttaaagt catttttcgc catcagagta atactcccca tgctcctaca tcaaaaccac
     3421 tgatgaattc gttatccata tcaaaaccaa aaaattttga gccttccgat ggggtttcca
     3481 ccgaaggtgt ttcaatgcca cccgcccata ccccggcggc ttttactgtg agatacccct
     3541 gtttaattgc cgcaattaac tcacgcgata catctgaaat atcagtatca ggaaagaccc
     3601 agaccgatat cgtcatgtcc tggttatcga ctatctgcat tcgcagcccg tctcctgctg
     3661 ttgcagcgtc aagaattgcc ggaagcgaat cattccgtcc atcccagtta ttaatcgcaa
     3721 tcttcgcttt aagaatgaca cgataagttt catcgctgag atacatgtat cctgaatcag
     3781 gatcgtatgg tccctgccat accccctgat catatccaag cccgtcggta tcccagctga
     3841 aatagacacc tgagataggc tggctgacaa cacggctacg tccgatccac aatccaagaa
     3901 tgtcaagttg cacaccaacc gcagagtcaa tatcaaatgc agtaatcagc cctctggtgg
     3961 cagccgcaac atcaataagt ggccgggtca tcagatcaac atgcgcaaga aatttaggtt
     4021 tggtggcgtg gtagttcgtg attagttcgg tgtatttgct catgactcca ccgttataac
     4081 gatattttcc ggggtacagg acgcagattc gttgtatctg atatcaatgt ttgatgacga
     4141 caaagccccc ggggatttcc caatcgtcag ttcctgaata tcgtaatagc gtgcattccc
     4201 gctactcacc acgccaagat tcgccggtga gtaaatgcga cttaaaagga ccgaatcacc
     4261 aatcatcaga ctattgatat agtcggaaat agcctgctgg atctgctgcc ctatctgtga
     4321 ggtataaccc gtaaaaactt ttaatttaat ccgggcataa acaggcacat cactggaacg
     4381 cgagaatttg attacatggg gattgccgta tttatccgga accgtaacgg atgttgtacc
     4441 gtgagtggct gtcccctggc ctttatttcc tctgatagcc tgagcaatat ccgttacatc
     4501 accgccatcc acaattacag caacagagtg tggcggtaac ccgttaccgt cctccgaacc
     4561 agtatcgttt tcatagagtt tgtggcgggt tacaccggta acattagaaa cagcaccatc
     4621 cagtgcttca aatggggtta ttgatggcaa cgcaacactt tgcgactggc ggatacgtaa
     4681 ctcagcgtca gtttctgccg gagtgcccac agtagccgca gcaggattag ttaccgaaac
     4741 ccagccacgg gttggcgtat taatttcagt gatagttcca gccagcgccg ccactgcacc
     4801 actgacggaa catgttgcgg tcaccatcac tgtaccatcc acgccgacca ccactgaagc
     4861 aggcaaacgc catatcacat tattactgtc tttcacgctg ccattaatga tggttgttcc
     4921 ggcagttcct gtaagaagca aatcaaccgt agaattcgtc gcgcctttac gtgaaatacc
     4981 atttattttc acgttactgg tcagtgcggc tccatagccg gttgctggtg aaaaacagtt
     5041 gtagacagtt atcgccatat tattggcatc atgaatcgcc agcgccatca gagccaccat
     5101 ctggccgtct ttgctgtccg gttcgaggta ggcatcactg ccataaatct gctgaaaata
     5161 gctaatcagg gtgctgagta tcgtctgata atcaggcgca ctgatcccct ctgcggttac
     5221 ctttgcagat aaaccgagag aatcaaggtt cagagccatt acgcctccga tgtaacagtc
     5281 gttattccat agagagtgtc gatttcagtg gaaaacctga cacgtcgggt cgtggtatcc
     5341 accgtagtat tgaaagagag gattgattta acgccccgcg tttcgaggat gcgcttacgg
     5401 atcgccaggt tgtaggtttc cggcttctgc ttaccgagta cggactggat ccacggagtc
     5461 ccctcggtgg tgtcgagaaa ccattgccca taccacaatt cgaatcgcgt ttttaccgcc
     5521 tgcgccacgg cctccggtga gttaatcagc caggtgtcat caccgctgcc aaaggtgtaa
     5581 tcgccatcgg cgtcttcacg tctgtatcgc atcagtttac cccatcggta ttgcttccac
     5641 cgcgctgaac accgccatga gtgtgcgtat catcgattgg cttgccgtta gccttaacgc
     5701 tccccaagaa ctcaacagca ccagtgattt ttgaagccac accagaaaca acagacccca
     5761 ccatgccccc catccaggtt aacaggccat gaatggttac tttctcagaa aaatcagcca
     5821 gaggggcaac cacatcaaga ccacccggag cgacaatttt aattttcctg gtgtcaggat
     5881 taagctcaaa ataggtgctg ccgtcgtcac tacgcaactg tgtggcactg gtattaatac
     5941 cgctaatctt cctcgcctgc gactggggac cgacaataca aaacgcatcc gataaatcat
     6001 gcattctgtc atcgaccggc tcctgtatcc cgccactctg ccaccagaaa tcaatacaac
     6061 gatcggcaaa aacgacaaga cactcatccc cggcttttac cggaaaagtc aacgtacagc
     6121 ctccgccgcg cgggaatacc actggtacat ccaccagcag cgggtaattt ttggttacgc
     6181 ggttgccgtc gttgtcggtt tcaaccgaac gaatagcagg ctgcacaacc gccgtcaccg
     6241 cgtcaggatc gaatgactga acgatgccag gcaaagcgac gcggatctgg ttctttgtgg
     6301 tttcccgctc ggatttgaat gtttcggcaa ggtcgccgct gcgggtctgg tcagatactg
     6361 ccattttgta ggctccagaa agcaaaaaac ccgccgggtg gcgggtttga taacgttaat
     6421 tttattcatg aagccctagc agactttagc tgttcccagc gctcaaaata gagttcagca
     6481 gcctcgattg catcctgtcc catctctttc gaaacagtca cattcgtcag atcatagtca
     6541 gcttcgtttc tagcatcccg ccactggcga agtacgaaag ccagggattt cagtgtattt
     6601 gtcgggtatg gctctgattt gcactctgat ggcgttgaca tataacccac agtatttttg
     6661 tgatgctcat gggtaaaatg cggtatggca gtaagtgagc taatagactc atgtaacata
     6721 ctgtaatacg cccttgaaat agcgcttcga aatccactct caatattttc agacagacat
     6781 aatcgagcgg tatcgagaat ctgattgcta gttaccgaca tggtagctca cccccgtatg
     6841 caactgacgc ggagaggtaa atctagctaa cagggaacaa tcatccagtt tagattcagc
     6901 acagactcta tcagccaaat cgaaattcat tttggcaata gttttaggct caagattttt
     6961 aatatcaaca acatagcaac cgcttccact aagctgaacg aaacctgtcg tagccttgta
     7021 ttcacttagc aagccccaga ttatggatgc taaggtctga aactgttcct tcgagcactg
     7081 tgtggcgcta taaaaattat ccatttctgc aaggagttct tctttgtgct tcatagcatt
     7141 actcctgcct tctgattcag aaagaagctt aatgtgctct tccatataaa attctaactg
     7201 ttcacgctca ccgcaacggt acgcccaact gtatgcgttg tgactaaaaa gttttgtccg
     7261 atacttcttt gccagcggga catagacatc cttgagttca ttatagctaa atgtattaat
     7321 taaaatgtga tgatagtgca ttgccaacgt tacatcgttt acttccaggc ttatcctgag
     7381 ataacgtaac gcgctttcga tctttccggt tattgcatcc agataagata gtgcaatgtt
     7441 ggtagcaggc gttggagaga cagaaagttc ctctcgaatt ttcttttcag tgaagggatc
     7501 taatctttcc ctatccaata gaagcgcatt gaattgttca acatattttt gcgctttttc
     7561 tagtgggatg cctgccgcca tggattattt ctcttactaa agggattgtg attgtgaact
     7621 ttgaacaata tctcgtcaac cttaaagcgc tcatctcgga agattgacat caaactttag
     7681 cgtaacgcct ttttacaagg gaatgatccg atgattctag gcgcatccat gctgttctgc
     7741 agaagctgga cgttcagaaa agcttttccg ttacgcttca caaactcaaa gccgtaattg
     7801 ttgccatcgc gggaaggcat caggcccatg tcggttttaa cgttgtccca gtcatctttc
     7861 tggccaagaa acttaatttt ctgagatgtt acctgttcgc cgttgatttt cgtccacccg
     7921 ttatcagatg catgaagtcg gtatcctccg cattgcaaat cagcgaaagc cgatgagcag
     7981 gaaaacagta acgaaattgt cagaactaac ttcatacgct tcatgaataa aacctttgct
     8041 gcgctgagga cgaaagtaaa tccgccgcac cacgcgcttc gcacatcatg tccatgtacc
     8101 acgcctggcc ccttgtgtca ccagtgtaca taatcccgcg cacaatataa acgccatccg
     8161 ttgcgatgct ggcaggctgc gatgtggtgc cgctgagcgt gatatttccg tccgtgttct
     8221 ggtcggtgat ctgcccaccg gccatcgcga tatcgttgct cgacagtgcg gtgcgatata
     8281 cggaagcctg atcaagctga atgagcccgt taacccggat gttcggatta atcagcgcgc
     8341 ggacgtttac gccgttaccg atagtctgct gcggcatgcc aataagcccg gtagcgctgt
     8401 tgagcacaat cgcttcgtga acatattcgt tattcgccac catctggcgc tgaccgtcca
     8461 cgaattgcca tgttgcgcca cattgcccgg ctacgttatc cataagatgc cgcgtcatgc
     8521 caaagagtac ccgcccccgg gggaatacag tagcaggcat ttcaggcgtc agaccttcag
     8581 tcgcgccttt ggcctcgaag tctttcatca gcgcgcgatt cacgtctgcg accgtataac
     8641 cggccgccag cgtctgcgaa gttatgctgg tggcaaaagc cagatccgta tcggctgcct
     8701 gaatcagaac gtaagaatca atggggctgt cttttcctgt taccgagtaa cgaatttcgc
     8761 cgctgaaaat catcccatag ttgcggccat cactctggcc cacatccgcc gcatcgactt
     8821 cccgcacggt cccgacgtcg ctggccgata cctccggcgc gataccgtcg taacccgcaa
     8881 tcaggcgcac tttcgaaaac tcctgcccgg tgattcggtt cacagtatct gccgagaggt
     8941 tataaatttt gatagtccct acccgggacg cgctgctgat gttgaaccag tcgatcgtaa
     9001 aggtgacttt gaaatcactt agctcaattc cctgaccgtt cccgtccaca agctgcagct
     9061 cgaaatgtct catccagttc tgtgacatgc ttactccgtt gataccagta aatgactgcg
     9121 accgcccagg tcagttttcg tgggataatc ctgtgtgttg tcatcgcaga ccaccaacag
     9181 cttaaaacca agccccatac aggcgtactg cgccagcaga tcagcgccag tgacgagagg
     9241 aataccggag attaccggct ctcctctgtc gttctgcagg tccataatcc agtaaggatc
     9301 tcgccatatg atgctaatcc gccaggtggc accacccagg acgatgctga actgctggtt
     9361 gtccgctgtc agcggaattt cctgaattgt cattagccgc cccccagtaa tgacgccacg
     9421 ttacccgtga tgcttttcag cagtgaagta tctggaggct ttgtggtttt gttgccgcta
     9481 ttctgtaccg ccgacgtgct ggccccttcc ttcatgttgg ttttatccgc gacggtaatc
     9541 tgctgtgtcc gggagataag gacctccctc agggtgagga cggcggacag gacgttttcg
     9601 gttgtcttgt ccgtcgtcac ttccagcgcc cggatcaaca tgttgctgta cagtcgttta
     9661 ccggttacca catcgaaggg gatacggctt tcctgcagat ccagtagctc ctgatacgtc
     9721 tgctgaggac tcaggccgag caggctggta gccgtcagat tactggcaaa atccagcaat
     9781 gcgccgccac cggcgaaacc aacctccatc accacttctg acggtttttt ataggcatga
     9841 tcagcgacag cggccccgac ctctaccgga tgctctgtta tttcaagcat atctgtatgc
     9901 ttctctgaaa taacaacact gggaacaatc attcctattt ttctgctctg ctgatgaaaa
     9961 agtgtagaga gaatatccac taacccaccc tcacctgatt acttcgcatg acctgagcat
    10021 ttgcagactg ttgccgacgt gcaacctcat taccgacagc gtgcggatct ccgccaccgt
    10081 aaatgtggta agtattttgc tggttaacct ctgtcatttt gccactaatt cccgccacgg
    10141 cagccttatt aatcagctct cgagaataga tatttcttcc attttcatgc tggataatgc
    10201 tgctcatcaa tgctgacatg gtttgcggat ctctcatatt cagggcagcc cggggatcca
    10261 ctcccagtcg ttgcgataca gccctgatat acgcagttgt gttgttatta tcagacgcag
    10321 gtgcccaggt agagataatt ttctccacac tgtttattcc ccgtccggcg tacagcatta
    10381 actgacgagc aagagcccgt aatccatcaa aggcagtttc aaatctggca aatcgcccgc
    10441 ccgggcgttc aagagaagcc cctgcctgac cagcaaaatt aaggtttccc ggattgttat
    10501 tccgttctcc tcgtttcgta gcctgtgcat gttgttctgg ctcatcatca ccaaaccagc
    10561 cgcgtaccgt ccggcccacg ctgcggggat cgaatcccca gtgctcttta atccagtcgg
    10621 cagtactgtt agcgctgtct gtaaccatcg gcatcgctga cggattttcg ctgccctgat
    10681 taagtatctg tttgccgatg ctgacggcat cagcccagcg gccatctttg atagcgttga
    10741 gcaggtcggc gatcatgttc agcattttgc tgaattcgcc catctggtcg atgaatttgc
    10801 tgaaatccca cttcagggac catgatttga ggtcaatatt gagcagtttc gccagcgctt
    10861 tcaccaggtc gttaacggtt gttttaaggt cacgaaccat cttcagcgcg gcatcgacct
    10921 ccggtttcca cttgccccag tcaatcaggc tgtcgccgcc ttccttccag gtctgatagt
    10981 cctcccacag gagggcaatc cccgccgcca gcgcggtaat gaggccaatc ggcgacatcc
    11041 agaacgtact gttcagaatg cgcagcgcaa tcgtcagtgc gccaaacagc gagatcaact
    11101 cccgcgtttg cttatccagc gattgccacc aggtgataag gcctgatgtc ccctcaatca
    11161 gtctgaagaa cagccgcccg attatatccc cgagcgccag aatgcctttt atggctttcg
    11221 tcagagtctg ctcgatgcgc gggaagttgt ccaggatatg gcggcgcagg gtgtccagcg
    11281 accccgcaag tccacccgca agattagagc cgattttgtc acgggccatg cctgccatcg
    11341 cgctaaactc acgcagggag gtcataaatt tgttggagct tctggccgcc tcgtcagcat
    11401 tgaagccgat cgctttcacc atttctgaat actgagcgct gaactgcccc actccgcgac
    11461 gcatagccat cagggtattt tcgtcaatgc ccagcatctg cgcatactgg ttagcccggt
    11521 aatacggcat gctgctgagt ttctgtccaa cgcccgtaaa gatagcggcc atgtcacgca
    11581 tgttaccgct ggcatcacgg gtctgtacgc ccaggcgatt cagaaagcct tctgcaccgg
    11641 gattgttacg aataaaccgg gagaggcttt ccagagaaga tcgcgcagcg tccacgctgc
    11701 cgccaacctg cgaaaccgca tagccaatag actgaattcc ctggactgtc gcgccggtgc
    11761 gctgtgacgc ccagtaaaga ttatccaggc cggaggcgat cttagccgtg aaggccacca
    11821 cggacagcgc agctccttca acagccagcc ccattttgat gacatttgca gttgtaccgg
    11881 cgaggacaga accgaacttt ttcgctcctg catcatccac actgaagcca agcgagacga
    11941 ggaaatcttt aatagtttca gcgttcatta tcctctctcc atttctcaat gcgtcgctgg
    12001 ttatccgctt ttaccgccag atggtcattc aagagagcaa tgtcatacaa atcgacagag
    12061 ccatctttaa gtgctgtata aggaattaac ccggcgtcaa ccggattgag aaggtaggac
    12121 agcccgtccg gcaggctgtt aaacgtcagc cctgttgcag gctctgcgtc gtgctggtaa
    12181 ggggtgtagg caaaaaattt cccagcgaat cggcgaccac ccgcgccacc agctgcagca
    12241 tgaccagcaa gtcaatatca tcaaacatca gttcgccctg ggtaaatacc ggcacccatc
    12301 tgtccatatg acgccgcgat accaccgcaa gacagggatg aataattgca tcggtgtcat
    12361 cttcggtcag ggaagacagt tcctcagcga tacgcgggag catggtttca aacaccggtt
    12421 ttaactgttc gaatttcacg gtgtcgattt tgccgtcagc aggcaaacgg gagcgaatac
    12481 tcccgaaatc tgacatcatt cctgccagca ccggcagaag tttgcgggtc actttcagct
    12541 ggtcaaaaac gctgagtttt gccgtgcgat atttcacgcc tttaatttcg aattccatgc
    12601 attaaaactc cccgagaacc tggtcaatct tgccgcagtc aaacacccac ggcatcgtat
    12661 taccggtttt agcgttggcg ttatccggtt gtttctggaa cgcaacactg cgtgccgtga
    12721 tgatgtcgcc gctgaccttg ttgcggatca cgataacgtt attcccccat gtggcagaag
    12781 actggctctg tgcgttatac gccagcgaca attttttatt tgtcggtgat gtcttcagaa
    12841 ggttaacggt aatcgtcccg cttttatctg catggagact gtgcatcact tcgccatcag
    12901 caccgatggt catggtgttt ttaggaccgc ccatcgcaac cacaatcccc tcttcagaac
    12961 ttgcagaacc gtacccgagg tcaatcgaac cggtcggccc ggtcagcgtc gcagtgacat
    13021 ccataaaaga ataggtagac attcacttcc ccttagcgaa caacgttaat ctgtacgtca
    13081 gcgtaatgaa ccgcgcctgc aagttttatt gcagcctgaa tcaccggagc cttacgggct
    13141 tcacgttctg attgtgcctg ttcatccagc ggctgggcgt atacgtaata acctttgggc
    13201 agcgtgtcac ctgatgacaa ctgaccaagg tcgccaccgt tccatacgcc cggagcaatc
    13261 agtccattct gaacagcctg atccagtgat ttttcaacat ttgataacag tcgggtaata
    13321 ccggcttcag tctggggaac tttcgtggtg ctggtataaa gcaggttata gaggttggtc
    13381 tgcacataat tctgtaacca gtccaggccg tggcgttcat caaagaaatc gccgttagcc
    13441 atcactccct gctggaggat agccgtatca ttctggtagt acacgaatac attgcagttt
    13501 tttgcatcaa gtaccgatgc ctggctgacg gtcagtgttt catacccgac acccggctcc
    13561 tgcttaaact tgagcgtaat cgcggtatta ctgccattga aattaaccgt gaatgcccgg
    13621 ccaaatgcag ataacgcagc gtatttatta cccgatgaat actgaataaa actgcgtgaa
    13681 tatccggcgg ttttcagttt tgatgccaaa tcatcgctgg atgcagtctg caggcattta
    13741 tcatcgcttg tcgtaatcgc cagaatacgg cttacagaag aggattcgat cgccgcagcc
    13801 actttcagcc agtctgcatt cggaatatct tcatcgtctg caatccccag cccataccat
    13861 gaagtataat cgagcatggc attcacagcc tgctccagcg tctcaggcgt ggcctgttcg
    13921 ctgtctccct tcgttttcac ccaacgacca acaaaaacct cctgagtttt cggtgattga
    13981 gagaaaaaca cctgcgcagc cttatattct ggtgattcca cgccaaaatc ttttccaata
    14041 tcttccgcgg cagaataacg gcgaatgcgc tcacttaccg gaatgattgt ggacgggccg
    14101 agaatgagta atgcaccaaa atttcgccct gatgctgcac gcggcgacat gatcacatca
    14161 acattaacaa cgtttgatac aggcaagccc tgtgccatag cttaatctcc gaaaaagatg
    14221 actggtgctt ccaccagcga tttaataccg tactcgcgca caaccttccg gcgcagacgc
    14281 accgtcatat cgtagcggcg gacccattgc tgattaataa gttcagggaa gggagtcaga
    14341 cctgtgtaat cgccaagaga cagccccaac gcattcagtg ctgcattgtt ctgcggtaca
    14401 gatataccgt cacgaaaccg ggacgcatac accatccccg ccggtccata aaacgaagcc
    14461 atacactcaa tcgtttcatg ccgccagagc tgagagccat catcggtctg tctggtgaat
    14521 gccgggctgt catcacctga ccattcgata accccaaacg cacaccagtt cgtttcagcc
    14581 ggtagcagtg gcggctgctc tttctgccag cgcggacgaa ccatcccggc agacagaccg
    14641 gaaacgttac gcatccactg gcttaacagc ctgtcgagag cttcgtcata atccggatca
    14701 ccactggttg gtatcagcca tccgagctct gtactggtgt tattgctcaa ccggagttcc
    14761 cccatcaaac ggcatcaact cacaatgcgc ctgaacgaat ccggccccat aagctgtata
    14821 cgggtcgacg aaggtcacac gataatcacg gccctgatac gtcacgatat cggcatcacg
    14881 cccagtctgt ccctgcgtca gtcgctcagt cgtcacaatc agaattgcac cgctgattac
    14941 ctgccctgcc tgcatacggc ggttttccag agagcgatca acagttacga ctccggcaaa
    15001 ctgcttttta acttcactgt cgctgccgat tccgtcctca tccaccgttt gcacacggcg
    15061 tgttacccac aaattgaagt cgcaaaaatc ggggtcaaaa agcacatctg ttacatcaag
    15121 agtcggcatc tttatccctc acaatatggg taatggctct gcgatattgc ccggtatcaa
    15181 ttaatggttt cgccagttcg gttcccggag attcgccagc agcacgccgg gcaagttcca
    15241 gtgttgctcc cttgcgcccc cgacgagccc gggcttcaac agtactgtca gcaagcggcg
    15301 taaagccggt aatggtcatg taacgcctga cgccattaac ggccagcgtt ccggcacggt
    15361 tgagtgcact ttctgctccc gcagcattac catcaagtgc agcctgcgcc gcggttttta
    15421 gctgcggtac cgtctgctct tctgccgatt taacaccggg gaccaggtga ggtcgtggcg
    15481 ggatgttctg ctctggtgaa ccgtattcgt tgaggtaacc gatgcccgca ttaccaaacg
    15541 gaacatcatc ccgctcgctg tcttccgaag ggatgccgac cagcacatct tttttggtta
    15601 acgacctgag cgcatccaga atggccttag cgttatccac cctcgttgtt acaccgcttt
    15661 tgaaactcat agctggcgac cgcctgcacc gaacatcgtg atcaactgat aaaattcagc
    15721 gccatatcgg gtgttattcc agaaacctgc atcaggattc agcgtcgcgc tggtgtcata
    15781 actgacgctt accttatcca cggactttga ggcctgaaca ccattggttg aaccgcccga
    15841 accaccagcc agcatcgccc ggctgtctgc cgcccagagc gtcatgtagt gcgcaacgaa
    15901 caacccggca aagtacggaa acaacttttt gccggtgacg ttttcgctca gcagttcatc
    15961 ggccagattc agacgaaact ggatttgcgc ttcgggatat ttggcagggt cagcaaactg
    16021 cgggaagtcg cggcgaaaat cacttaccgc tggcagactt tgattctttg gcatttttta
    16081 cctcgttacg cgcgtctgtg gctttgccaa cggatacttc cgcgtgcgca cgagtgaacc
    16141 agtgcgtggc aacgtcttcc tccacagcat gacggccttt aacaaactcg cgccgagaac
    16201 cgtcgggaag cgtgagcaca aacggggtat gtacgtgtat tactgcatca ttttttgcca
    16261 tcgggtcatc cttaatggcc ccgccagggg gccatgtggc tgttaaatgc catcaacgta
    16321 cgaaatggtt tctttgtaca ctggctcaac cgcacccagc ttgccgtagt aagtgacgat
    16381 ctgatacaga ccgcgatact gcaccggcac gctctgaagc ggaaccagcg ggtagcggac
    16441 gtatttttta tcgttggtgt acgcaaccat gcgatccttt ttccccacac cacggccttt
    16501 cagccattta accgcgcgga tattcagcgg aacaccgttc tggtgatagc tgatggtgtt
    16561 ggtctgaaga tacgtcaaca gggactggtt acccgcagat gaaacgatga tgctggacaa
    16621 cagagcaaac tgctcaggcg ggatcagcaa atcacgcgga accacagagt aaccggaagc
    16681 ggcccacgca tcagacagca cctggttaat gcttgcgcgg atttcgtccg gtgttgaagt
    16741 tgcccacgtt ttggcagcgt tgttgacagg cacgcagtcc agggtaacaa ggcctttcag
    16801 gtttaatgct gaatcgccaa catatacctg ttcatcgtta tccatctgcc atttcagttg
    16861 cataccgtca tacttctgcg tatcaatcgg gcggccgacc tgctgagcag cctgcaattc
    16921 tatgaccgtc cagccaagtt ccatccccca caggttcagc gggttaccgg atttgccggt
    16981 atccacgttc acgccagcaa tagcggttga gtctttgcct acccagtttt tgccattcgg
    17041 atttgcacca gtacccgcag cggcgaagct ggtattcgtc cagctggaaa tgtcatctgc
    17101 gatggagacg tcttcacgca gttgaatatc gcgggtccag gtgtacccca ccagcggcag
    17161 gttcagcgtc tggtcgagtc gctccagctc cccgatgaga aaggcaccag agctgtcaac
    17221 ggttgcctga tcaaaagtaa tcattcgtct gttccttaaa tcttccagga aatttctgca
    17281 ttgccgtcag catcaccggc acctgtgaat tcagcgttgg tcagcaccac gtttttgcca
    17341 ctgactgacg tggacatgaa tccacccagc ggcactttga tggattcatc agtggagacg
    17401 acaacgtata ccgggtcgcc ttttttgatg gtgctggcat caaaatcaga accgagatta
    17461 acagtcacgt agccacgctt catggcgtcg cccgggaagt tcttgcctgt tcccacctgg
    17521 cgaaccatgt ccggctgcga cgtggtcgga taagggcgca cgtagatccc cttcaccttg
    17581 tctgcggtat caccatctgc cagcggcacg aaaaaaccgt catcatcgta tttaccagcc
    17641 aggccatagg cagcgaaggc gttatcggat ttaaggacca ccggttcgac ggttaagtcc
    17701 tgcgggcgag agacagcccc ggcaatgcca acaggcatcc ggtacagaaa tacattattc
    17761 attttttacc ctttacggtt tgcccagaat tcagcgtttt gtttgttcag ggaagcgata
    17821 ctggtcatgc ccatgtttag gcgctgtgca tcgccggtgg tggcgcgggt gtttcgccct
    17881 ttggcaatct cagacacggc attaaacgcc atgtcgaccg attgtttcgg caatttgcgg
    17941 atatccgcat caccgacgat ctggcgaacc agcgttttat ctgcggaagc cagaacctcg
    18001 cgtttgaact cggtcggttt catcttacgg ctcagatcga tacccggaac gataacttca
    18061 gcacgccagg ctgagtcacc agtaatcgtg gtttcctgtt catcgtcctc gccgtcaccg
    18121 gtcggattat cgtcaggctt attgtcgtta tcgcccgtcg catttccttc cagcttagcc
    18181 agcagggctt tcagtaatgt tttgagatca tcatcactgt cgccggttgg acctccgccc
    18241 atctctggtg ctttgtccgg tagcggttgc tgcggggaca ggttaatgtt gagattaacg
    18301 ccctgcggca aatccccctc atctcctgta accgatgcgg gagccgactc caccagttcg
    18361 ttcatggtgt cagcgtcacc cgttttgatg gccgtgcgca tgcggctcca ccagcttttc
    18421 ttttgatttg ccattgtgtc tctgtctcca attgcacaac gatttccggc tctgccttta
    18481 gggacaagag ccacatggtt tccggtaata tcgacctgct cggctttacc tggctcggtc
    18541 tgctcatact ccgcgtcata gccgcacgac acttcgcgca ggccatcttc gataagctga
    18601 atggcgcttt cgtctttgac gataaggtca gccagcatca aatcagactg ctcacccgtc
    18661 ccgcgccgga cattctggag gtgcccgaca gcaagctctt tccagttctc gggatttacc
    18721 agccgcacat tcccgttttc atcttcagga tgcaggatcg tgatactcat cccttcgaat
    18781 gaggcaagcg tggccggatg gaatacctgc tcaggagaac gcgtgacgac tatttcaccg
    18841 aacttatcgg gtttcagttt tggcaggtca tcagcaccat agagctgctt acctgttcgt
    18901 cctatcggca cgtctttgca cagcaacgag ccgtcagcca gctgatagcg ggtttccccc
    18961 agccgggtat tgaaaaaata tttcatgggt tacctgcgat tcaggcggga taagaatggg
    19021 aggagggaaa aacgatttct ttataacaac gacaattcgg gagctcgcca gcgtgaccgg
    19081 tcatgccgtc aagcgttgga ggtttgcccc attcgacaaa tttaccttcc atttcccgat
    19141 gagaatgcct gacgtcacca tcttcggctg tacgccagat ataaccattc gaaccaattg
    19201 acagcgcacg cgcctgatcc agcgcgccgg ttgcacgtcc aagttcagta cgggcaatca
    19261 ggtcagctct ggactttgct atatcacccg atgcggctat ttctttagca aaatgttccg
    19321 ctctcccacc ggtaacaaca gcttctatcg cccgattctg gatgtcgtac accctgtcag
    19381 ccgcctcgag gggtagcgat ttgatgtact taacctgttc ggcaacgatg gatttcatca
    19441 cctgcccagg cggggaactg tttaccagat tgcgtagctc acggctgatg gttttgctgt
    19501 gtttacgcca ctgctcatca ttcttgcgca caatgtcggc ggtaaagttt tccgcgacct
    19561 ttgtcgccca gggggtgatg atttcactgt agcgttccag cgcctcaata atttccgtga
    19621 tactgtcatt tgaaccatcg tagtgaccat ttacgatgtc cccgaccgcc cgcgctatcc
    19681 tgcgtaggct ggttcgatac cggatttccg cctgacggtt cctgcggttc gtcatcaggt
    19741 tcgccgatgc cgggcggcgc ttcatcttcg gcattctcga tgtcctcgtc ggtaatggat
    19801 gccccgatgc cggttacgtc agaattttcg cgcaaatcag tcatagcggc tttcagtgtc
    19861 atcagaccat cacccagcgc tgtactgatt gcgttggtgg tatttaacgc caccgttgaa
    19921 cgatcgacat cagacatttg ccagagcggg ttaaactcaa acgtgaaatc atccgggagc
    19981 ggcttgccaa gttccgaacg atgcatgatg tccagtatcc gccgcaccgg aagacgtaaa
    20041 cgcctctcct gcaatgagct tacccggtcg taatagttgg caagatctgc atcaccggta
    20101 gaaaatcctt tcggggactg tccgaacaac cgcaccagtg ggataccaac agcgccgcta
    20161 atctgttctg caaactgcga aaggatgtca tccagaccac tgaagttgta ctgatgggtt
    20221 tcgaaggtat ctttggcatc cattagcgtc atgccttcat tgctctgaaa ctgtcgaatc
    20281 agatcaatgt ttttcagcag cgcctcgaat gcctggccgc ccagcgcaat aagctcacga
    20341 agtttttcca ccttgtaggt ccgcagatgc gctttgtaga ccagctgcgc cgcgccgaca
    20401 gtagcgctat cgaacgcggt aagacgatcc cagatacgct ctacaaccga cattccccat
    20461 tcgttctcgg tcatcttctg ctggaatggc agcgtgacgc catcaaagcg aatcagacga
    20521 ctgtgatgaa tgcgccaggc aggaattccc gttgctgtgg tcaccacatc gtaaaactca
    20581 ggtttaccca ggtccggccc catatcttta atgcggcggg tcagcactgg gttaatcatc
    20641 cagcggtcga gcgggagaat gcccttaaac ttgccttctc caatggtttc gagccgcagc
    20701 ggggtcattg gtgcctgccc ctcaatcatg atgaaaccca ccgcgccgcc atagaggcgc
    20761 gaccatttca gcacgtcatt cagcgcatcc cagatttgca actcatccag ttgtgattcg
    20821 agaatgccgc gatcttttgc atcaatttcc gaagtgatgc gaatgccttt gcgggtcata
    20881 tcatctggga tagcatcgac cgcttcgccg atgatccagg acgaacgata ggaccattcc
    20941 accagcatgc ggttacgact ggtgaaatta gcccggtagg tggatgctga gtgctggtta
    21001 ggtgtctgca tccctacgcg ggcaataaaa ttctcataac catcagctgt ggcctgcgca
    21061 gttcgccgca gggcttgttt gtttcgtgcc atcaggcctg tctccctagc agctcccaga
    21121 tgttcagggc tgaattcatt ggggcatagc tgatcatcac cgagtcggca aggtttggcg
    21181 accgggttcc atcaggctgt ttatcaataa cgatttttcc cacaccatta atggaatagg
    21241 tcggctgcga aagctcgatg atgagtttat ctttgagtgc catgctactg ctgattgaga
    21301 tgatttcgtc cgggttgtaa gccatacctt caaccacggc gcgccaggta ttctgaaaaa
    21361 gtttacgtaa ccgccaccag ctctgggctt tggcgttagc gaagaagtcc ttgttcagac
    21421 gtgcggcttg cccgttgtcc ccgcgaacag cttcatcatc cggatcaaat accgcgccac
    21481 tacctcgaaa cggtgtggca agtattgacg gtcgacgcgc agcgttacgc agttcgttga
    21541 tagcgcgtgc atcgccgcga acgccagcgc ccagcccgtc ctcgtcaaag cgaaactctt
    21601 cgaggttgtc ctgttcgcaa aagccgaaaa ccttctcgac ggactgataa atgtcgctgc
    21661 ccacaccgga ccattcccgc acattttcca ggaggaagcc atgacgggtg gaaaaggcat
    21721 ttttgtccct gccttcgtcg gcgacatcca tcgcgccaag tcgtttgcct gttggctgga
    21781 tacccagttt gatatgcgca tcaacggcag cctgtaccca ttcggatgga atcagaacgc
    21841 cttccgctga tgcgctgtag ttcagatcaa gttcctgtgc caccaccacc ggattatcga
    21901 ttttctcgca ttccctgcga taccactctt catccttgcg aggatcatcc cgccagtgga
    21961 atgtgaatac cggtatcttc ccgccatgac gcttctgagc gaacgggtta gccatgccgt
    22021 taactgaact caggtcgata cggcaacgcg tcgtttgtga caacgccgca tcaatcagca
    22081 gaggacgctg aaggaatgca gcctcatcaa ccagataaag cgtggtacgg tcaccacgac
    22141 caatattatc gccagcctcg cctttgataa cggcaccagt ttcaggaaac tcaacacgca
    22201 tatatggcgc gtgcttcttc tcactccacg aaccgcgaaa ctctacaggt agcgtttcca
    22261 cgaacttgcg tgccttccag aacaatgctt tcgggtcacc agtgctgtcg acgtattcct
    22321 ctttacggga gccgaaaccg ataaccattt ctttgttgaa gagacaaagc gagcaggcca
    22381 gtccgatcgc ggtccaactg agccccattt cacgggattt ttcggtaata ccattctccc
    22441 gattgcccca gcgttccata atccagtgga tccactcctc ctgcttaggg aagagtaaaa
    22501 acggaatggt caccggcagg ccataatcaa tattacgcgg gtccgttgtc atgccccagt
    22561 cgatgatgaa ctgagccgga ttggttcggt aaaactgctt caatacgggc aatatttcag
    22621 gattctggcg aatgcgctgt aggcgttcca tccgccattc aaaaaccatc tggtaatcag
    22681 gatgtttaaa atcgaagggg aatggtaacg gcatacttag cccatcattt ttctatacgc
    22741 ctctgcagcc tgctccggcg ttaagttggt aatttctgtt ctgacgggtc ctccatcagc
    22801 gccagtcact tcatttttga cgttgtcttt aaacgcctga acagaaacat gacgcccaag
    22861 caactcaagg tttttaacct tatcaggcca cttaatcttt ttaaggatcc cgaccatttc
    22921 tctgtcatct cctcgcccct caaacatttc agcgaggtta aatccactca ggtaccgacg
    22981 ccacgattcc ggccacgcag acagaggctt aatacttaaa tcgtcctcca ggatgtcagc
    23041 cacatcgagc ctgtcgatct caaccagtcg catcagcaca taattcgcat caatgcccag
    23101 ttgatcaata cgctcctgct ttagctcgtt aatacgggcg cgtatctcag gtttaccgta
    23161 tagttcagcc cccgtaacat gtgctcgcct ggagacgtag cctgcgcgaa tagctgcttg
    23221 tgtagcattc agatcgacaa gaaactcgcg acaaaacacc tcgtgttttg ctttcagctt
    23281 cttagtcatt ttattttcca gttatcaggt cattatcgaa gcccctcctg gaagagcttc
    23341 tgtaatgcta ttaccgggac tgttctattt gtcggacacc agccaactgg ttattcgcct
    23401 tctcgatggc cgccaacagt gggttaatcc acagaacagc ctggcaatat gtcaacgttc
    23461 tggtggtagt ggcacgatca ccggctgagt caatgtcccc ggaatcggcg tgcaatgcgc
    23521 tggcgcgtaa acggttcgcg tagttgagca acccgacagc aatatcaaca ggaacaggga
    23581 aatcacagtt cttttcacgg agcagaacct cacggtattt gatgactgtc ttctcgtgac
    23641 cgatgtcgac cagagaattt aatcggcttg cgttttctgc tatctggtta aaacgattga
    23701 agtttaatgc ctgattagct atcactttcc cttgatattc agcttcacct tccgctttat
    23761 cagcccgcaa cttttctgcc tgatacttgc tgtggtaatg gtttgcagac cagacgagcg
    23821 ccccaaaggc agagaagaaa aatgccgcga tgacaatctt ataagtcagc ttcatttacc
    23881 accccaccag catcattaaa cctggaaatc aggtcaccga ttttatgttc atactgaccg
    23941 taacctgcac caggtaacga cgcccagata ttgctgcaac ggtcgattgc ctgacgaata
    24001 tcgccacggt caatcatcgg taaagcgcca cgctctttaa tctgctgcag cgccacagcg
    24061 tcctgacttt ctggcgaaaa atctttcagg ccaagctgct tacggtaggc atcccaccag
    24121 cgtgaaagaa gctgataccg tcctgcggct gttgatttaa gcttcgggtt tagcgtgaca
    24181 agtttgcgag ggtgatcgga gtaatcagtg aacaactcac ctccgacgat aacgtcataa
    24241 ccgtgattac gtgtcggttg tcgcccgtta tccgttcctt ctgaccatgc caccatatcc
    24301 aggaaagctt tacgttggga atttagtgtc tgcatgaatt actccttaga gccaccaaac
    24361 ttgttaccga ttactcgcat tgcagcccca cgaatagcat ccacaccgat cagcccaaca
    24421 ccaccaccaa tggcaacaga aagcgattta ggccatccaa catactcaag agcggatgca
    24481 aaggtcagcg tcagagcacc acagagcaaa atctcaagcg tttttcgttt ccagccgccg
    24541 ccaccgccaa aataggcaat gcgcaagcca gccataacaa ttgacataac cactgcgccc
    24601 aacggcgtat ctccacgcca ccaactttgt aagagttcca gtaagtcagg ccaggaatga
    24661 ggagcattgt gcattttcat acttcccacc tccgccatta cggggtgttg ttgaaaggcg
    24721 ggccctgcgt atacgcccgt aggattgggt tatgagccgt ccttcggtgg gccctgaata
    24781 caaaaaaagt tcgccatagc gaaccttgtt aaatttgtta aaaaaggaag ttatttaaca
    24841 taatgtacgt tataggaacc acacgatcac cgctcgcaat agatttgcga tgaaaggcct
    24901 atttaatcaa cttaagtggt ccagaatgac gaaattcgag tgaataaaag gtgcataaaa
    24961 aagggcaaaa actgcatagc gttttttcgc agcgaaagcc ctgttttatt aacttttccc
    25021 cagaatgggg caataaaaaa ggcccccttg cggaagcctc ttcgtgatga actgttttca
    25081 acggatatca ggctaatcgg ctttttggaa ttccacaact attttttatc ttaccttgat
    25141 gcctgatgcc gtcagatgtt tacgacagtc ctgaatacct tcgttataac ccgcatcgta
    25201 gaagctatcc ggctttgcct tcagcgcagg aagcgtaacg ctggtagctc ttcgttcggc
    25261 cagaacacct gcctgaaaga gtgtccacat caactgaact gctgaatcac agtaagtaac
    25321 atcttcactg tacaattctc gttcaatcct gatgccaagt tgagccttca ttgatgcgct
    25381 aatattacct ttgacctgca acgacaggtc tgggtacctc tgttccatga agctttcaaa
    25441 tctgttgcga acgctgatca gagccatctg gttcccttac gtgattgatt gctggatatt
    25501 atgccgccga gcagctccta cgcaaagtcc tgatagcctg aggctttgac tgaaccgact
    25561 gatccatttc gagcaccaca tccagcatgg ctaagcaacc gtcaataaat ccctctgccg
    25621 cctgaagcct cttcagaacg tgggtgtgag atacgccgag cttctcaccc atcgcccgca
    25681 cgggcatggc ctggacgtaa tgccactcaa ggagcgtgca cagatacgga tcttttttct
    25741 tcaggcagct catagcggag ttgatgataa gaccgtcatt gtcactgcat ttcagccttc
    25801 cagactgcgt cgcgggtagc agattcttaa acccggctgc aacaggagga aagtagacgc
    25861 taccaccttc actcgctgcc cagccacccc agcgctctaa aacctgttga atattacgca
    25921 tagctctgac ctctacgttt tgctacgaaa aaattttctg agaaccacgt ctgtttatcc
    25981 gtgggtgtgg ataacgcagt tccgattcgc ttattacacg ctgttatgtg caaaaaattc
    26041 cttgttcggc ctgtaccaac ctgtacccac ctgtaccagc tcttttcaaa ccttttcccc
    26101 aagcgactta tacatatata tggggttctt agtaattagg ttggtacaga tggtacagtt
    26161 ggtactgacc tttaaattca attagttaaa atgtaccaac ctctattttg aggctggtac
    26221 aggttgggac accgcctcaa aaattcgagt cattttcccc ccaattcgcc tttgggcacg
    26281 cttatacccg caattttgca aaacattact aattcgcatt tcttcgcgtt ttccgatgcg
    26341 gtcaggattt agcccaatcg catcacgcaa aacatcactt gcgcgtaaaa attcgcaatt
    26401 tcgcggaagt tcattagtca tcaggtctgg cgtgtcgagc catttctcta ccgtttcaag
    26461 ccaagcatcc ttgatggtgt actgttcatg gacactcgct gccagttgtt cagcctcacg
    26521 gaactggata ccccccagac gctgaaacac ctcacgagcc tcagcccaaa gtaaaaggag
    26581 atctcttttt atcgctttca cgtcgacttt cgacacttcc acggggagcc agcgacggtt
    26641 accagtcttg tccgcaagga attcgtcctc attggtggta ccaacgaaca ccaggcgacg
    26701 aggaaactgg gtggcgaact cccggtattt cgggatccag ttttcgtgag tacgcgtcac
    26761 aaacgctttg attgactcca gctctttggt attaagtccg cgcagttcac caatctctgc
    26821 caccagccga ccacgcattt ttcgagcgag atcatcgtct ttttcggcaa aagagatttc
    26881 agtaaaaaac gcaggatcag ggctcagtgc ctccactccg gaagacttac cgcagccctg
    26941 cggaccaacg aggatcggca ccatatccgc tttgatgcct ggctccagta ctcgccccgc
    27001 caacgcggtc cacatgtaca tagacaccgc acgggtataa ggcgtgtcgg cggtaccgaa
    27061 gtgcgtatgg tagaaacatt cgatgcgcgg caccccatcc cactccagtc cgttcagcca
    27121 ggcgatcgct gaatcgaatg gttgttcatc ggctgcaagt aacaccacat cgcgaataag
    27181 ttcacggcca acaggtttaa atccccgctt ttccatcgtg atgcgcaggc gcgcataatc
    27241 cgcatcggtg aatgcccgcc attgtccgga tcctgccggg gcaaacatga tttcgtcgcg
    27301 gaacaggtca aagcgaatat cgatgtccac aaaatcaggg cgtactactg ctttggctgc
    27361 gttgctgatg gttgcctcga tacgccccca tttatcacgc tcgaaagcag gcagcggtaa
    27421 aggttccgcc acttcggtgc tggtcagatc ttcgaaatcg tcgttgcgga tcccgatggc
    27481 attaaggaaa tctccgtcat cacgatgcgc acaactggca tgcaggcatt taaaatgccc
    27541 ctgctcaaag cccgcggttc ccgcaggaaa ataaactgtg cttgttggat cgcccccgct
    27601 actgtggccg tcttcaaacg gacagcggat atatcgttca ccatttgcac catccagcag
    27661 cgtccagcca ttggcatcaa gatattcagc tgtatcatcc gtcgcgccgg gcgtgaatgc
    27721 tgatcggttg cgcatcttcg tgttgcccgc ttcggtggtt accgacacag ggagttgttc
    27781 agccaggcgc tgccacagcg tttcaagctg ctcaccagta atagccggag gttcatccgg
    27841 caaaccgccg tcccattcaa tacgcgcgcc gctgctgtgc gtaccgcagg caacgaactg
    27901 ctgcccgttc gccagcaact cgataatgcc catatccccc gccaggcgat ggatacgctt
    27961 acggaaatca ccatcaacgg ccagcagata cagacactta ttactgtttg ctcgccagcg
    28021 tcgtggcggc aactcaccca gaagttgcac aagcgtttta cgaatatctg cctgaatgtc
    28081 ttcatcttcg ctatcacagt ccagcgccag ccagccatgg cctgtacgca cgcagatgcc
    28141 ataatccggt tcattcgacc agcgggcaaa atcatgctca gtaacaacat gcccggtcca
    28201 ttgagcaata ccggtgacct ggcggtcccg gttatagcga ctcggcgtct tacccaacgc
    28261 tttcagttta ctatcagggg atatggtcgc accggggtta catacaactg gcagaagatg
    28321 atcagttctc cccaatacca ggtcgaaatg aaaccactca tcaggcgtag ctccccatgg
    28381 tttgctatcg gacatgggtt aagccttttt atcgttttgt gagccataca acagccaatt
    28441 agggtcgcaa tccaatgcta tagacatttc aagaagataa cgtggccgag caataacacc
    28501 actttcaatt ctgtttattg cctgctggct cactcctgtg agctcagcca acgtaacttg
    28561 cgtcattttg agttctttac gtcgttcttt tactcgggtc gctaaggtca ttgctttcac
    28621 ctcatacaat tttagtggta tttagcaaca acaaacgagg tttgtcaaat acaacaaaaa
    28681 ttgtttttaa tgtggagata cacattaata caacccagaa ggactaatcc catgtctctc
    28741 gcagcccgtt ttaaagcacg tcgtttggaa ttaggaatga ctcaagtgga agtagcgaat
    28801 tctgcagggg ttagccaaca atccatagaa tccatcgaaa gtggacgaac cagaaagcca
    28861 cgcaatcttc tggatctggc taaggccttg aaatgtagcc cagactggct actgaacggt
    28921 aaaaatatta tgcctctcgc ggagataagt accagacgca tacctatatt aagttacgtt
    28981 caggcaggag agcttacaga agcaagagac ataacagatc tgacagggga atttgaatat
    29041 attttggcag actctgacat tccagaaaca tgcttcgcat tacgtatcga tggcgacagc
    29101 atgcaaccag agtttaaaga aggagacatt gttatcatcg atcctgatct atgtcctgca
    29161 ccaggggagt ttgttgtcgc caaaaacgac ggtcacgaag ctacatttaa aaaataccgt
    29221 ccattaggaa tcggcatcga cgactttgaa ttaatccccc taaatcctga ttaccctatt
    29281 tttcgtagtg cagatatgaa cttacagatc ataggtgtaa tgatcgaaca ccgtatttac
    29341 cgccgtaaac gttagtattc ccaccgtaaa tatcgggagg cgtaacagcc tcccttaccc
    29401 catttgtaaa gtcttacaaa ttaaattcac ttaaatatca ataaagtagt attcacaccc
    29461 cacaaaatac cacatttgtg gtttacacaa tacaactcaa attgtatctt tagccacaag
    29521 tcgaacggcg cgactctaaa ccatgcgtcg ggaccgtggc gggacaggat gtcggcaata
    29581 cgggtcagta agttcccttt ggggtgcggc gaagcactgt ctactgatac aagtcgaagc
    29641 tgccgaacca ccaaagtgaa ctgaatgagg aaacggcgtg aaaacgtata aaccgctcaa
    29701 aggtgaatgc cctgcttgcc aaacacagct tcagacacca aagccaaaac gataccaaat
    29761 caacgaatgt tatgagcact gccctggatg tggtgctttc ctgcatacca ttgcaacgca
    29821 ctggcgtgtc cgcttcaacc ttgtggtacc acgtacgcac aataccaact gaatcatcca
    29881 tgtaattgct gtgtgtagtc tttgcccgcc gcaagtgacg ggctttttta tgtctgaaag
    29941 cgcactcgca acagcgcgct ccccgatatg aaaaaaggaa tacaaccgat gaaacctgaa
    30001 cacctctatc gactgacggg gcgcgatgtt ctccgttatc gccgtaaaaa cttcgatttg
    30061 atgaccggtc tggccgttgc cactgcgctc ggtctgatca tcactttcat tctccttgta
    30121 gcgaggacca cagtatgagt ttagaaacca gtctcgaact taataatcaa cttctggcac
    30181 aacataatgc gctgcttgaa cgtcttatcc gcacaatggc atcaggtatt gttatgcatc
    30241 ctgacacgat ttcacgagtg caggaatgtc gggatacagc aactgaaact gaaaatatgt
    30301 cagcggcaat gacactggat gatctggagt tcagcgacgt tatcgcactg gctggtttct
    30361 acccggtagc cacccctatc acagaagaca tgctgcaacg tgctgttgcc taccgtgatg
    30421 ctgaaggcga taaacgagta gttcagattg atgctctcga cagcgcattg cagggcgtca
    30481 aacgagccag ggcgctgctt aaaccagctc tcctggacct gtcccgtaac atccttaagt
    30541 tctgggacga cctgccaacc atcggcgagc ggcgtgcttt tgccgagcag ctacttgatg
    30601 cacctgcgga tgggcgtgat gaagttaagc cgaaaaaggc cagtaacaaa gatggagaac
    30661 gcacggggcc gttttacgtc aaaaatgtat ccggcacagc agccagtgaa ctccacacct
    30721 tacgcaagtt gaacgagatg ctgaaaaaag gccatatcga gatcaaccgt gttgagtacc
    30781 ttcagctgca ggaagaattt gcacgcagag acgcagcaaa ttccagccag aataatgacg
    30841 ccaaagatga ccatacaatt gatttcgcgg cactacgcaa acaagctgaa gggttgatcc
    30901 tccagttagc aaaagggggt taccgggcag aagctattgc aattctggaa aaacagggag
    30961 ccaggaaact tggtgaagta acggatgaaa atctcgctga agtaatcacc ctggctgaaa
    31021 aagcactgga gggttaatca tgccagacgt tcatgcacaa ctttctccat catcagcgca
    31081 tcgatggatg cgctgcccag gaagtctggc gctggaggct acacaaccgg ataaagaaac
    31141 aacttttgca atcgagggca ctgcagcgca cgcgcttgct gaaaaagttc tacgaaacag
    31201 gcaaagccat ccggaacact acgccggatg caatgtttct atgtttctcg gctcataccc
    31261 ccttcgcgaa aatcctgatg atacatctgg cccacaggtg gatgatgaaa tggtcgaagc
    31321 cgttggccgg tatgttgata cggtctggac tcttgcacag aataatgaac tattggttga
    31381 acaacgtgtt gatttctcac atataacggg tgtggaagaa tctttcggaa ctgccgacgg
    31441 cataatcatc gcgggtaacg aattacaaat ccacgacctg aaatatggca aaggcgtccg
    31501 cattgatgca gaacaaaatg agcaactaca actgtatgct ctgggtgcgc tcgaacaatt
    31561 cagcatgctg tatgactttg agactgtaag attatttatt caccagccaa ggctcaacca
    31621 cgtttcagaa tggtcgttaa ccgtacagga acttcagtct ttcggtgaac gggcacagga
    31681 ggccgcaacc agtgcgatcc ttgttctcaa tattgctgaa tgcgaaggca ttgagacact
    31741 accgctggaa aacttcatac ctggagaaaa acagtgccgc ttctgtaaag caaaagctat
    31801 ttgcactgcc cagaaaatgc agcatttaca aacagcggcc agcgattttg aagatctgac
    31861 aaagcctgtc agcgaaataa tcaccaatgc cagtgcacgt gtacctctgt taaccattga
    31921 gcagcttgcg gagatctata gccaggccga ctttattgaa tcctggctaa aggcagtacg
    31981 ggaccgggtt cacaatgaac tcaatgccgg acatccggta ccggggttta aactggtaac
    32041 aggaaaacaa ggtaaccggg cctggattga tgaagaggca gctcgcgcac ttctgaagga
    32101 ccagttcagg tacaaaactg aagaagtatt cgactttaaa cttattagtc ccacaaaagc
    32161 cgaaaaactt atcaaaaagg ccagtccgcg ccgttggtca aaagtcgagg cactgataac
    32221 acgagctgat ggtaaaccca ccgtcgttcc cgagtcagac ccacgccccg cactcaatat
    32281 caaccctgta aatgatttcg acgacgtatc cgacgatacg ctaaccgcag acctcatctg
    32341 atttaaggaa atacccatga aactgaagct gaacaatgtt cgtctggcct tcccgtctct
    32401 gtttgaagct aaaactgtaa acggcgaagg cgatccacgt ttctccgcag tatttttaat
    32461 gtcacccaaa cacccacaac tggaagaaat ccgtaaagct atgaagcagg tagcgaagga
    32521 aaaatgggga gagaagtggg agcccattta taaccaactg gagaaaaaac tcaatctgtg
    32581 cctgcatgat ggtgatgaaa aagcagagta tgaaggcttc cccggcaatt tcttcctgaa
    32641 tgctgctaac aaagcgcgcc cagctgttct tgatcgcgat cgttcgccac ttattcaggc
    32701 tgatggacgt ccctatgcag ggtgctatgt aaacgccgtt atcgatatct gggcacagga
    32761 caataatttc ggtaaacgca ttaatgcctc actcggcgga gtccagttcc tgcgagacgg
    32821 cgatgcattc gctggcggcg gagtggcaag cgctgacgat ttcgacgata tcagcgaagg
    32881 tgctgatgct gaagcactga tttaacccta cttcatagac gcccggttaa ataccgggct
    32941 gctgattagg cgtataatca ctcaggaacc ttaatcttcc tcaattcttt atttagttca
    33001 cagatcttct tcttagacgg gaaagtatca tacatccaaa aaccaacagt ctctaaaact
    33061 attgtcataa ccatatacac aagataattt aaaaggcctt cattttgccc tatggaaata
    33121 atgcagataa tagaaaaaag gataaacagc acaaaacaca gaaaggaaaa cagacgacta
    33181 ataataaata aaaaacctga aaaattaaca ttgatgaa
//
LOCUS       DAZFYC010000044        32675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFYC010000044 DAZFYC010000000
VERSION     DAZFYC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.044"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..3844
                     /gene="yfaL"
                     /locus_tag="ACHKW5_002348"
     CDS             110..3844
                     /gene="yfaL"
                     /locus_tag="ACHKW5_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO7511733.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLKNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3972..4694)
                     /gene="ubiG"
                     /locus_tag="ACHKW5_002349"
     CDS             complement(3972..4694)
                     /gene="ubiG"
                     /locus_tag="ACHKW5_002349"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO7511734.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4841..7468
                     /gene="gyrA"
                     /locus_tag="ACHKW5_002350"
     CDS             4841..7468
                     /gene="gyrA"
                     /locus_tag="ACHKW5_002350"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO7511735.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7617..9305
                     /gene="yfaA"
                     /locus_tag="ACHKW5_002351"
     CDS             7617..9305
                     /gene="yfaA"
                     /locus_tag="ACHKW5_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO7511736.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9302..9925
                     /gene="yfaT"
                     /locus_tag="ACHKW5_002352"
     CDS             9302..9925
                     /gene="yfaT"
                     /locus_tag="ACHKW5_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO7511737.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9949..14463
                     /locus_tag="ACHKW5_002353"
     CDS             9949..14463
                     /locus_tag="ACHKW5_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO7511738.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDVNGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14464..16113
                     /gene="yfaQ"
                     /locus_tag="ACHKW5_002354"
     CDS             14464..16113
                     /gene="yfaQ"
                     /locus_tag="ACHKW5_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO7511739.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVGLFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16118..16894
                     /gene="yfaP"
                     /locus_tag="ACHKW5_002355"
     CDS             16118..16894
                     /gene="yfaP"
                     /locus_tag="ACHKW5_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO7511740.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17169..20018
                     /gene="rcsC"
                     /locus_tag="ACHKW5_002356"
     CDS             17169..20018
                     /gene="rcsC"
                     /locus_tag="ACHKW5_002356"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO7511741.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20218..20868)
                     /gene="rcsB"
                     /locus_tag="ACHKW5_002357"
     CDS             complement(20218..20868)
                     /gene="rcsB"
                     /locus_tag="ACHKW5_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO7511742.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20885..23557)
                     /gene="rcsD"
                     /locus_tag="ACHKW5_002358"
     CDS             complement(20885..23557)
                     /gene="rcsD"
                     /locus_tag="ACHKW5_002358"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO7511743.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTWDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            24296..25399
                     /gene="ompC"
                     /locus_tag="ACHKW5_002359"
     CDS             24296..25399
                     /gene="ompC"
                     /locus_tag="ACHKW5_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7511744.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25511..26566
                     /gene="apbE"
                     /locus_tag="ACHKW5_002360"
     CDS             25511..26566
                     /gene="apbE"
                     /locus_tag="ACHKW5_002360"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO7511745.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSTLMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            26640..27704
                     /gene="ada"
                     /locus_tag="ACHKW5_002361"
     CDS             26640..27704
                     /gene="ada"
                     /locus_tag="ACHKW5_002361"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO7511746.1"
                     /translation="MKKATCLTDDQCWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVVMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRSLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            27704..28354
                     /gene="alkB"
                     /locus_tag="ACHKW5_002362"
     CDS             27704..28354
                     /gene="alkB"
                     /locus_tag="ACHKW5_002362"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO7511747.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28430..30073
                     /gene="yojI"
                     /locus_tag="ACHKW5_002363"
     CDS             28430..30073
                     /gene="yojI"
                     /locus_tag="ACHKW5_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO7511748.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            30291..31937
                     /gene="mqo"
                     /locus_tag="ACHKW5_002364"
     CDS             30291..31937
                     /gene="mqo"
                     /locus_tag="ACHKW5_002364"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO7511749.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(32086..32595)
                     /gene="eco"
                     /locus_tag="ACHKW5_002365"
     CDS             complement(32086..32595)
                     /gene="eco"
                     /locus_tag="ACHKW5_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO7511750.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         7939 a         7988 c         8878 g         7870 t
ORIGIN      
        1 taacaaaata gccattgcat cgggttggac aatttacaaa agaaagataa cccatattgt
       61 ataatgcgtt taaatatgcc gtcttatata aaatgatggt tagatgaata tgcggattat
      121 ctttctacgc aaggagtatt tatctctact cccgtcaatg attgcatctc ttttctctgc
      181 taacggtttc gcggcggcca ttgatttatg ccagggatat gatatcaaag cgagttgtca
      241 cgccagcagg caaagccttt caggcattac gcaggactgg agtgtcgccg atgggcaatg
      301 gctggttttt tcggatatga ccaataacgc cagcggagga gccgtatttt tgcaacaagg
      361 agcggaattt tcactattac cagaaaatga aactggaatg actctgtttg ccaataacac
      421 cgttacagga gaatataata acggcggggc catatttgct aaagaaaact caacgctgaa
      481 tcttactgat gttatttttt ctggtaacgt cgcaggcggc tatggtggcg caatctattc
      541 ttctggtact aatgatactg gtgccgtcga tttacgtgtc actaacgcca tgtttcgcaa
      601 taatatcgct aatgatggca aaggtggcgc aatttatacc attaataatg acgtctattt
      661 aagtgatgtt atttttgata acaaccaggc atatacatca acaagttaca gtgatggcga
      721 tggcggggca atcgatgtta ccgataataa tagcgacagc aagcatcctt caggttatac
      781 gataataaat aacactgcct ttacaaataa cactgccgaa ggttatggcg gggcgatata
      841 taccaatagc gcgacggctc cctatcttat tgatatttct gttgatgaca gctacagcca
      901 gaacggcggc gtgttagtgg atgagaacaa cagcgcagca ggctatggag atggtccttc
      961 cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa gttacctttg atattgccga
     1021 cggaaaaacg ctggttattg gcaatacaga gaatgacgga gctgttgact ctattgctgg
     1081 taccgggttg atcaccaaaa caggttccgg cgatctggta cttaatgcag ataacaatga
     1141 ctttactggt gagatgcaga ttgaaaacgg tgaagttacc ctgggccgca gcaactccct
     1201 gatgaatgtc ggcgatacgc attgccagga cgatccgcaa gactgctacg gtctgacgat
     1261 agggagtatt gataagtacc agaatcaggc agagctaaac gttggctcca cccaacaaac
     1321 ctttgtgcac tcattgacgg gctttcagaa tggcacttta aatatcgatg ctggtggcaa
     1381 tgttactgtt aatcaaggca gttttgctgg caccatcgaa ggtgctggtc agctcaccat
     1441 tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg atggcgctaa ccggcgatat
     1501 agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac gcggcagatc tcgccgctct
     1561 ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg ctcgatctct ctgatttctc
     1621 cacctggcag agcggcacat catacaatga tggccttgaa gtcagtggca gcagcggaac
     1681 ggttatcggc agtcaggatg tggtagatct tgcaggtggc gacaatttgc atatcggcgg
     1741 cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc gacgggcagg taagtctggc
     1801 aaacaataat agttatttgg gcacaacaca aatcgcctcc ggtacgctga tggtgagcga
     1861 caactcgcag cttggagata cccactataa ccgccaggtt atctttaccg ataagcaaca
     1921 agaaagcgtg atggagatta ctgccaatgt cgatactcgc tctacgacga ctgagcatgg
     1981 gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt gatgcggggg tagacacgca
     2041 gtggggcgca ctgatggctg acagcagcgg gcagcatcag gatgagggta gcacatttac
     2101 taaaacgggg gcgggtacgc tggagctgac cgccagcggt acaacgcagt cggcagtacg
     2161 tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc tttccttatg cttcgtcact
     2221 gtgggtcggt gatggggcga cgttcgttac tggcgcggat caggatattc agtctatcga
     2281 tgctacttcc agcggcacta tcgatatcag cgatggtacg gttttgcgtc tgaccgggca
     2341 ggatacttcc gtcgccctta atgcctcatt gtttaacggc gatgggacgc tggtgaatgc
     2401 taccgatggc gtgacgttga caggtgagct taataccaac cttgaaactg acagcctgac
     2461 ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat acgtccggtg cggttagcct
     2521 gaaaaatggc gtcgctggcg atacgctgac ggtaaacggt gattataccg gcggcggtac
     2581 gctactgttc gacagcgaat taaacggcga tgactcggta agcgatcaat tggtgatgaa
     2641 cggtaatact gctggcaaca caactgtggt ggttaactcc attacaggga ttggtgagcc
     2701 gacatcgaca ggcattaaag tggttgattt cgcagctgat cccacgcagt ttcaaaacaa
     2761 tgcacagttc agtctggctg gcagtggcta cgtcaacatg ggagcgtatg actacacgct
     2821 agtggaagat aacaacgact ggtatctgcg atcgcaagaa gtaacgccgc catcgccacc
     2881 tgatccagac cctgacccaa cacccgatcc tgacccaaca cccgatccaa tccccgctta
     2941 ccagccggtg ctgaatgcca aagttggcgg ttatctcaat aatctgcggg cggcaaatca
     3001 ggcgtttatg atggagcgac gcgatcatgc aggtggcaat ggtcagacgc tgaatttacg
     3061 tgttatcggc ggacgttatc attacacggc ggcagggcaa ctggctcagc atgaagacac
     3121 ttctacggtg cagcttagcg gtgacctgtt tagcgggcgc tggggcgatg atggcgagtg
     3181 gatgcttggg gcggttggtg gctacagtga taatcagggc gagagccgct cgaatatgac
     3241 cggaactcgc gccgataacc agaaccacgg ttatgccgtt gggctgacct caagctggta
     3301 tcagcacggt aatcagaagc agggggcctg gctggatagc tggctgcaat acgcgtggtt
     3361 taacaatgat gtttccgaac aagacgatgg cacagatcat taccactcgt cggggattat
     3421 cgcctcgctg gaagccgggt atcagtggtt accggggcgt ggtgtggtga ttgaaccgca
     3481 ggcgcaggtg atttatcagg gcgtgcagca ggatgatttt accgccgcta accatgcgcg
     3541 cgtgtcacaa tcgcaggggg atgatattca gacgcggctg ggtttacaca gcgaatggcg
     3601 taccgcggtt ggtgtcacac caacattaga tctgaattat tatcacgatc cccatgcgac
     3661 ggaaattgaa gaagatggca gcactatcag tgacgatgcg gtgaagcgac ggggtgaaat
     3721 aaaagtggga gtcacgggca atatcagtca gcgagtgtcc ctgcgcggca gcgtggcgtg
     3781 gcagaaaggg agtgatgatt ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg
     3841 gtaatcactg tctagtcggc acggttgccg gatgcggcgt aaacgcctta tccggcctac
     3901 ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat tggctcggcc
     3961 aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt tcacatccac gccggggccg
     4021 agtttaaaag tattagtgat cgggttgtaa tgtagcccgg ttatatgtcg ctctttaagg
     4081 cttgtctgat ctacccagcc cagcaattct gccggtttaa taaacttctt cacatcatgc
     4141 gtgcctttgg gcaccatgcg caaaatatat tccgcaccaa ccaccgccat cagccatgac
     4201 ttgccgttgc ggttaagtgt cgagaaaaag acatcgccgc ccggtttcac cagttgcgca
     4261 caggctctga ccactgactg cggatcgggg acgtgctcca gcatctccat gcaggtcacc
     4321 acatcatact gcccggcatg ttttgccgcg tgctcttcca cggtttcctg cacgtaatcc
     4381 acctgaatgc cgctttccag tgcgtgcagt tttgccacct gcaatggctc aaagcccata
     4441 tccagaccgg tcaccgtcgc gccttcgcgc gccatactct cggccagaat gccgccgcca
     4501 caaccgacat cgagcacctt tttgccaaat aaaccgccag cacgctcggc aatatagccc
     4561 agacgcagcg ggttaatgcg gtgcagcggt ttgaactcac cttccagatc ccaccagcgg
     4621 gaggcgacgg cttcaaattt agcgatctct tcgtggtcta cgttatggtt taccggcgat
     4681 ttttcggcat tcattggcac ttctactccg taattggcaa gacaaacgag tatatcaggc
     4741 attggatgtg aataaagcgt ataggtttac ctcaaactgc gcggctgtgt tataatttgc
     4801 gacctttgaa tccgggatac agtagaggga tagcggttag atgagcgacc ttgcgagaga
     4861 aattacaccg gtcaacattg aggaagagct gaagagctcc tatctggatt atgcgatgtc
     4921 ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc ctgaagccgg tacaccgtcg
     4981 cgtactttac gccatgaacg tactaggcaa tgactggaac aaagcctata aaaaatctgc
     5041 ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat ggtgactcgg cggtttatga
     5101 cacgatcgtc cgtatggcgc agccattctc gctgcgttac atgctggtag acggtcaggg
     5161 taacttcggt tccatcgacg gcgactctgc ggcggcaatg cgttatacgg aaatccgtct
     5221 ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa gagacggtcg atttcgttga
     5281 taactatgac ggcacggaaa aaattcccga cgtcatgcca accaaaattc ctaacctgct
     5341 ggtgaacggt tcttccggta tcgccgtagg tatggcaacc aacatcccgc cgcacaacct
     5401 gacggaagtc atcaacggtt gtctggcgta tatcgatgat gaagacatca gcattgaagg
     5461 gctgatggaa cacatcccgg ggccggactt cccgacggcg gcaatcatta acggtcgtcg
     5521 cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg tatatccgcg ctcgcgcaga
     5581 agtggaagtt gacgccaaaa ccggtcgtga aaccattatc gtccacgaaa ttccgtatca
     5641 ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg gtaaaagaaa aacgcgtgga
     5701 aggcatcagc gcgctgcgtg acgagtctga caaagacggt atgcgcatcg tgattgaagt
     5761 gaaacgcgat gcggtcggtg aagttgtgct caacaacctc tactcccaga cccagttgca
     5821 ggtttctttc ggtatcaaca tggtggcatt gcaccatggt cagccgaaga tcatgaacct
     5881 gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa gtggtgaccc gtcgtactat
     5941 tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt gaagcattag ccgtggcgct
     6001 ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg ccgacgcctg cagaagcgaa
     6061 aactgcgctg gttgctaatc cgtggcagct gggcaacgtt gccgcgatgc tcgaacgtgc
     6121 tggcgacgat gctgcgcgtc cggaatggct ggagccagag ttcggcgtgc gtgatggtct
     6181 gtactacctg accgaacagc aagctcaggc gattctggat ctgcgtttgc agaaactgac
     6241 cggccttgag cacgaaaaac tgctcgacga atacaaagag ctgctggatc agatcgcgga
     6301 actgttgcgt attcttggta gcgccgatcg tttgatggaa gtgatccgtg aagagctgga
     6361 gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa atcaccgcca acagcgcaga
     6421 catcaacctg gaagatctga tcactcagga agatgtggta gtgacgctct ctcaccaggg
     6481 ctacgttaag tatcagccgc tttctgaata cgaagcgcag cgtcgtggcg ggaaaggtaa
     6541 atctgccgca cgtattaaag aagaagactt tatcgaccga ctgctggtgg cgaacactca
     6601 cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat tcgatgaaag tttatcagtt
     6661 gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc aacctgctgc cgctggagca
     6721 ggacgaacgt atcactgcga tcctgccagt gaccgagttt gaagaaggcg tgaaagtctt
     6781 catggcgacc gctaacggta ccgtgaagaa aactgtcctc accgagttca accgtctgcg
     6841 taccgccggt aaagtggcga tcaaactggt tgacggcgat gagctgatcg gcgttgacct
     6901 gaccagcggc gaagacgaag taatgctgtt ctccgctgaa ggtaaagtgg tgcgctttaa
     6961 agagtcttct gtccgtgcga tgggctgcaa caccaccggt gttcgcggta ttcgcttagg
     7021 tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc gatggcgcaa tcctcaccgc
     7081 aacgcaaaac ggttacggta aacgtaccgc agtggcggaa tacccaacca agtcgcgtgc
     7141 gacgaaaggg gttatctcca ttaaggttac tgaacgtaat ggtttagtgg ttggcgcggt
     7201 acaggtagat gactgcgacc agatcatgat gatcaccgat gccggtacgc tggtacgtac
     7261 tcgcgtttcg gaaatcagca tcgtaggccg taacacccag ggcgtgatcc tcatccgtac
     7321 tgcggaagat gaaaacgtag tgggtctgca acgtgttgct gaaccggttg acgaggaaga
     7381 tctggatacc atcgatggca gtgccgcgga aggggacgat gaaatcgctc cggaagtgga
     7441 cgttgacgac gagccagaag aagaataatt ttacttcttc atgccaaaag ggagctatct
     7501 cccttgtttg aattgaaaag tccaggctgc aaagtctggg cttttgtcgt attagggcac
     7561 ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata cacaaggaat gtggcaatga
     7621 gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct tgtaggtttt ggcgcaatag
     7681 cactgctttc cgctggcgtc tgggcgttgc aatatgctgg cagtgggcca gaaaaaacgt
     7741 tgtcgccgct ggtggtgcac aacaatctgc aaatcgatct caatgagccg gacctctttc
     7801 tcgacagcga ctctctgagc cagcttccca aagatctcct caccattccg tttctccacg
     7861 atgttctgag cgaagatttc gttttctatt atcagaatca tgccgatcgt ctgggcattg
     7921 aaggcagcat tcgtcgtatt gtctatgaac acgatctcac gctgaaagat aagctctttt
     7981 cgtcactctt agatcagccc gcgcaggcag cgctgtggca cgataaacaa ggccatcttt
     8041 cacattatat ggtgctgatc cagcgcagtg gtttaagcaa actgctggag ccattgttgt
     8101 ttgccgctac cagcgacagc cagttaagca aaacggaaat cagtagcatc aagataaata
     8161 gtgaaactgt tcctgtttat cagttgcgct ataacggcaa taacgccctg atgttcgcga
     8221 cttatcagga caagatgctg gtgttttcca gcacggatat gttgtttaaa gatgatcagc
     8281 aggataccga agccacggcg atcgcaggtg atttgttgag cggcaaaaaa cgctggcaag
     8341 caagctttgg cctggaagag cgtgctgctg aaaaaacgcc agtacgccag cgcatcgtgg
     8401 tcagcgccag gttgctgggt tttggctacc agcggttaat gccttctttt gctggcgtac
     8461 gcttcgaaat gggtaacgac ggctggcaca gttttgtggc gttaaatgat gaatccgcca
     8521 gcgtagatac cagtttcgat tttacgccgg tctggaacag tatgcctgcc ggggccagct
     8581 tctgtgtggt ggtgccgtat tcacacggta ttgccgaaga gatgctttcg cacatcagtc
     8641 aggaaaacga caagttgaat ggggcgttag acggtgccgc ggggctgtgc tggtatgcag
     8701 actcaaaatt gcaaaccccg ctgtttgtcg gtcagtttga tggcactgcc gaacaggcgc
     8761 aattgccagg gaaactgttt acgcaaaata ttggtgcgca cgaaagcaaa gcgccagaag
     8821 gtgttttgcc gataagccag actcagcagg gcgaagcgca aatctggcgt cgcgaagtga
     8881 gttcccgata cggtcagtat ccgaaagcgc aggcggcgca acccgatcaa ttaatgtcgg
     8941 attatttttt ccgcgtgtcg ctggcgatgc aaaacaaaac gctgcttttc tccctcgatg
     9001 acacgctggt taataacgcc ctgcaaacac tgaataaaaa ccgcccggca atggtggatg
     9061 taatacccac tgatggcatc gttccgctct atatcaatcc acaaggcgtg gcgaaactgc
     9121 tgcgtaacga aacgttgacc agtctgccga agaatctcga accggttttt tataacgccg
     9181 cacaaacttt attaatgccg aagctggacg ctttatctca acaaccgcgt tatgtcatga
     9241 agctggccca gatggaaccc ggtgccgcct ggcagtggct acccataacc tggcagccgc
     9301 tatgaggcac gggctgctgg cgctgatttg ctggctgtgt tgtgttgttg cccatagcga
     9361 aatgctgaat gtcgaacaat ccgggctgtt tcgcgcctgg tttgtacgca ttgcacaaga
     9421 acagctccgc caggggccaa gtccacgctg gtatcagcag gattgtgcgg gcctggtgcg
     9481 atttgcggcg aacgagacgc tgaaagttca cgatagtaaa tggctaaaaa gtaacggttt
     9541 ctctagccag tatttgccgc cagagatgac gctaacacct ggacagcgtc aactggcgca
     9601 aaactggaat caggggaacg ggaaaaccgg cccctacgtg accgcgatta atttgattca
     9661 gtacaacagc cagtttattg gccaggacat aaaccaggcg ctgcctggcg atatgatttt
     9721 tttcgatcag ggcgatgccc agcacttaat ggtctggatg gggcgttacg tcatctacca
     9781 caccggaagc gccacgaaaa ctgacaacgg aatgcgcgca gtcagtctgc aacaacttat
     9841 gacatggaag gacacccgat ggatacccaa cgattccaat cccaatttca ttggcattta
     9901 tcgtttaaat tttctggcgc gatagcagcg tgcttgtccc tgtctcttgt gggaacaggg
     9961 cttgctaatg ctgatgattc gcttccttcc agtaactatg cgccgcccgc cgggggaaca
    10021 ttctttttgc ttgctgacag cagttttagc agcagtgaag aggcgaaagt gcgactggaa
    10081 gcgccggggc gtgattatcg gcgctatcag atggaagagt acggcggcgt ggacgttcgc
    10141 ctgtatcgta ttcctgaccc gatggcattt ttgcgccagc agaaaaacct gcatcgcatt
    10201 gtggtgcaac cgcaatatct gggcgacggg ctgaacaata cgctgacctg gctgtgggat
    10261 aactggtacg gcaaatctcg ccgcgtaatg cagcgtactt tctcttctca gtcacggcag
    10321 aatgtgactc aggcattacc cgaattacat ctcggcaatg ccattattaa accttcccgt
    10381 tatgtacaga acaaccagtt ttccccgctg aaaaaatatc ccttggtgga acagttccgt
    10441 tatccactat ggcaggctaa accggtcgag ccgcagcaag gggtaaaact ggaaggcgca
    10501 tccagcaatt tcatctcgcc gcagccgggt aacatttata ttcctctcgg ccaacaagag
    10561 ccgggactgt acctcgtcga ggcgatggtt ggtgggtatc gggcgacgac ggtggtgttt
    10621 gtttccgata ccgtggcgct tagcaaagtg tcaggcaacg agcttctggt gtggaccgcg
    10681 ggtaaaaaac agggtgaagc gaagcccggc tcagagatct tgtggactga cggtcttggc
    10741 gtgatgaccc gcggtgtgac cgatgacagc ggtaccttgc agttacaaca tatatcgcca
    10801 gaacgttcat acattctggg taaggatgct gaaggcggtg tttttgtctc cgagaacttc
    10861 ttctacgaaa gcgaaatcta caacacccgc ttgtatattt ttaccgatcg cccgctatat
    10921 cgcgcaggcg atcgtgtcga tgttaaagtg atgggccgcg agttccacga tccgttgcat
    10981 tcatccccca tcgtcagcgc cccggcgaag ctttcggtgc tggacgttaa cggcagtctg
    11041 ttgcaaaccg tcgatgtcac gctggatgcg cgcaatggcg ggcagggaag tttccgcctg
    11101 ccagaaaatg ccgttgccgg aggttatgag ttacgtcttg cttaccgcaa tcaggtctat
    11161 agcagcagtt ttcgcgtggc aaactacatc aagccacatt tcgagattgg tttagctctc
    11221 gacaaaaaag agttcaaaac tggcgaagcg gtcagcggca aactgcaact gctctacccg
    11281 gatggcgagc cggtaaaaaa tgcccgcgtg cagttaagtt tgcgcgctca gcaattatca
    11341 atggtcggta acgatttgcg ttatgccgga cgtttccccg tgtcgctgga aggcagcgaa
    11401 acggtgtccg acgccagcgg tcatgtggcg ttaaatctcc ccgccgccga taaaccgagc
    11461 cgttatttgt taaccgtctc cgccagtgac ggcgcggcgt atcgtgtcac caccaccaaa
    11521 gagatcctca ttgaacgcgg tctggcgcat tactcattaa gtactgccgc acaatacagt
    11581 aatagcggcg agtcggttgt gttccgttat gccgcgctgg aatcttcaaa acaggttcct
    11641 gttacgtatg aatggttgcg tctcgaagac cgcacgagcc atagcggaga gctaccgtca
    11701 ggcggcaaat cctttaccgt caatttcgct aaacctggca actacaatct gacattacgc
    11761 gataaagacg gcttaattct cgctgggtta agtcatgccg tcagcggtaa gggcagcacg
    11821 gcgcatactg gtacggtaga tatcgtggcg gataaaacgc tgtaccagcc aggcgaaact
    11881 gcgaagatgc tgattacctt tccggagcca attgatgaag cattattgac gctggaacgc
    11941 gatcgcgtgg aacagcagtc gctgctttcg catccggcaa actggctgac gttacaacgt
    12001 ttaaacgata cccagtatga agcccgggtt ccagtgagca attcctttgc gcctaacatc
    12061 actttttcgg tgctgtatac ccgtaatggt cagtacagtt ttcagaacgc cgggatcaaa
    12121 gttgccgttc ctcagcttga tatccgggtg aaaacggaca aaacccatta ccagcctggt
    12181 gaactggtca atgtcgaatt aacctcgtcg ctgaaaggta aacctgtttc tgcgcagcta
    12241 acggtaggcg tggtcgatga aatgatctac gcgctgcaac cagaaatcgc gccgaatatc
    12301 ggcaaatttt tctatccgct ggggcgtaac aatgtgcgta ccagctccag tctgtcgttt
    12361 atcagctacg accaggcgct ctccagcgag ccggttgcgc ctggcgcaac taaccgcagc
    12421 gagcggcgag taaaaatgct tgaacgtcca cggcgtgaag aggtggatac cgcggcatgg
    12481 atgccgtcac tcacaaccga taaacaaggc aaagcgtatt tcacgttcct gatgcctgat
    12541 tcgttaaccc gctggcgtat caccgcgcgt gggatgaacg gcgacgggct ggtcgggcag
    12601 gggcgtgctt atctgcgttc ggaaaaaaat ctctacatga agtggagtat gccaacggtg
    12661 tatcgcgtgg gcgacaaacc ggcggcagga ctgtttatct tcagtcagca ggataacgaa
    12721 ccggtggcgc tggtgactaa atttgcaggc gctgagatgc gccagacgct gacgctgcac
    12781 aaaggggcga attatatttc gctggcgcag aacattcagc aatctggctt gttaagcgca
    12841 gaactgcaac aaaatgggca agtgcaggac agcattagca caaaactgtc ttttgtggat
    12901 aacagctggc ccgttgaaca gcagaaaaat gtcatgctcg gcggtggcga gaacgcgctg
    12961 atgttgcccg agcaggctag caatatccgg ctacaaagta gcgaaacgcc gcaggagatt
    13021 ttccgcaaca atcttgatgc gttagtcgat ggaccatggg gtggcgtgat caacaccggt
    13081 agccgtctga tcccgctcag tctcgcctgg cgttcgcttg ccgatcatca aagtgccgcc
    13141 gctaacgaca ttcgtcagat gattcaggat aaccgtctgc ggctgatgca actggcgggg
    13201 cccggagcgc gctttacctg gtggggtgaa gatggcaatg gtgacgcctt ccttacggca
    13261 tgggcatggt acgccgactg gcaggccagc caggcgctcg gcgtaacgca acaaccggaa
    13321 tactggcagc atatgctcga cagttatgcc gagcaggcgg ataacatgcc gttattgcat
    13381 cgggcgctgg tgctggcgtg ggcacaggag atgaatctgc cgtgcaaaac gttgttgaaa
    13441 gggttggatg aagctatcgc ccggcgcgga actaaagatg aagatttctc tgaggaagac
    13501 atccgcgata tcaatgacag cctgatcctc gatacaccgg aatctccact ggcagatgcg
    13561 gtggcaaacg tcttaaccat gacgttgctg aaaaaagcgc agttgaagtc cacggtgatg
    13621 ccacaggttc agcaatatgc gtgggataaa gcggcaaaca gcaatcagcc gctggcgcac
    13681 acggttgtgc tgctcaatag cggtggtgac gctacccaga cggccgctat tttaagtggt
    13741 ttgaccgctg agcaatccac tattgagcgc gcgctggcca tgaactggct ggcgaaatat
    13801 atggcgacaa tgcctccagt tgttttgcct gcgcctgcgg gcgcatgggc taaacataag
    13861 ttaactggag ggggcgaaga ctggcgttgg gttggtcagg gtgtgccgga cattctctct
    13921 tttggtgacg aattatcgcc gcaaaatgtg caggtccgct ggcgtgaacc ggcaaaaacg
    13981 gctcaacaaa gtaacattcc ggtgaccgtt gaacgccagt tgtatcggct tatccccggt
    14041 gaagaagaga tgagctttac tctgcagccg gtgaccagca atgagattga cagcgatgcg
    14101 ctgtatctcg atgaaattac gcttaccagc gagcaggatg cagttctgcg ctatggtcag
    14161 gtggaagtac cgctcccgcc gggagccgac gttgagcgca caacatgggg catttcagtc
    14221 aataaaccca acgccgcgaa acagcagggg caattgctgg aaaaagcgcg taatgaaatg
    14281 ggcgaactgg cctatatggt gccggtgaaa gaactgacgg gaacggtcac tttccgccat
    14341 ttgctgcgct tctcgcaaaa agggcaattc gttctgcctc ctgctcgtta tgtgcgttcc
    14401 tatgcacctg cgcagcaaag tgttgcggca gggagtgaat ggaccgggat gcaggtgaaa
    14461 taagtgaact ggcgcagaat cgtctggcta ttggcgttgg ttactctgcc aacgcttgcg
    14521 gaggagacgc cgttacaact ggtgctgcgc ggtgcgcagc acgatcaact gtatcagctg
    14581 tcgtcatcag gcgtgaccaa agtctcagca ttacctgact cgctcaccac gccgttaggg
    14641 agtttgtgga aactgtatgt ttacgcctgg ctggaggata cccatcaacc ggagcagccc
    14701 taccagtgtc gcggaaactc cccggaagag gtttattgtt gtcaggctgg ggagagcata
    14761 acgcgcgata ccgcgctggt acgttcctgt gggctctatt ttgcgccgca gcggttgcat
    14821 attggcgcag atgtgtgggg gcaatactgg caacagcgtc aggcacctgc gtggttagcg
    14881 tctcttacaa cgctgaagcc agaaacctcg gtaacggtta aatcgctgct cgattcatta
    14941 gcgacgttac ctgcgcaaaa caaagctcag gaagtcttac tggatgtggt gctggacgag
    15001 gcgaaaatcg gtgtcgcatc catgctcggt agtcgggtac gggtgaaaac ctggagctgg
    15061 tttgccgatg ataaacagga aatccgccag ggtggttttg ctggctggtt aaccgatggt
    15121 acaccgctgt gggttactgg cagtggcacc agcaaaacgg tactaacccg ttatgcgacg
    15181 gtgcttaatc gggttttgcc agtgccgacg caggttgcca gtgggcaatg tgtagaggtg
    15241 gggctgtttg cccgctatcc gctgaagaaa atcacagcgg aaaagagtac gacggcagta
    15301 aaacccggcg tacttaatgg gcgctatcgt gtgacgttta ctaatggtaa ccacattacg
    15361 tttgtcagcc acggtgagac gaccttactg agcgaaaagg gcaaactgaa attgcagtcg
    15421 catctcgatc gtgaagagta cgttgccaga gttctggatc gcgaggcgaa aagtacgccc
    15481 cctgaggcag cgaaagccat gaccgtggcg attcgcactt ttttacagca aaacgccaat
    15541 cgggaaggcg attgcctgac catccccgac agcagcgcca cgcagcgtgt ttctgcttcg
    15601 ccagcgacca cgggggcgcg aacgatgacc gcctggacgc aggatctcat ctatgcaggc
    15661 gatccggttc attatcatgg cagtcgcgct accgaaggga cactttcctg gcggcaagcc
    15721 acggcccagg cggggcaggg ggagcgttac gaccagatac tcgcgtttgc ttatcccgac
    15781 aacagcctta gccgctgggg agctccacgc tcaacctgcc agttattacc caaagcgaaa
    15841 gcctggctgg cgaaaaaaat gccgcagtgg cggcgtatat tacaaggcga gacggggtac
    15901 aacgaaccag acgtgtttgc ggtctgtcgt ttagtctcag gtttccccta taccgatcgg
    15961 cagcagaaac ggctgtttat tcgtaacttc tttacgcttc aggatcggct ggatttaacc
    16021 catgagtatc tgcacctggc ctttgatggt tatcccaccg ggcttgatga gaactatatc
    16081 gaaacgctga cccgacaatt attaatggac taatgctatg cgaaagattt ttcttccgtt
    16141 gttactggtg gcactttcgc ctgtcgctca cagtgaaggt gtgcaggaag tcgagattga
    16201 cgcaccgctc tctggctggc atccggcaga gggcgaagat gccagttttt cacaatcgat
    16261 caattacccg gcgtcgtccg tcaacatggc agatgatcaa aatatctcag cgcagatccg
    16321 cggcaaaata aagaattatg ctgcggcggg taaagttcag cagggccggc tggtggtcaa
    16381 cggtgccagt atgccgcaga gagttgaatc cgatggttca tttgcacgcc cttatatttt
    16441 cactgaaggc agcaacagcg tgcaggtcat cagcccggat gggcaaagcc gacaaaaaat
    16501 gcagttttac tcaacgccgg gtacaggaac gattcgtgca cgtttacggc tggttctctc
    16561 gtgggatacg gacaataccg atctcgatct ccatgttgtc acgcccgatg gcgaacacgc
    16621 ctggtacggt aacaccgtgc tgaaaaacag tggtgcactg gatatggatg tcacgacggg
    16681 gtacggaccc gagattttcg ccatgccagc accagttcac gggcgctatc aggtgtatat
    16741 caactactat ggtgggcgca gcgaaacgga attgacgacc gcccaactga cgctgatcac
    16801 cgatgaagga tcggtcaatg agaaacagga aacatttatt gtaccgatgc gtaatgctgg
    16861 cgagctgacg ctggtgaaaa gttttgactg gtgatccatc ccttcacaca acctggctct
    16921 ctcttttttc agataagact gctgggcgat ctgccgacgc caggatataa ccattaacaa
    16981 gcgtcggcag attggtcatt tcttattttt cgtactgaga actgtccatc tactccggct
    17041 ttcctggtta aggcaatggt ttctaagcga tacgcattat tgcaatggtg aaagaacatt
    17101 gcggtaacac gcttttaccg ctaccttaac cacactccat cggtcacctg aggcggagct
    17161 tcgccccttt gaaatacctt gcttcttttc gtacaaccct gaaagcctcg cgctacatgt
    17221 tcagagcatt ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt ttttacatcg
    17281 ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga atttaatctg agttccgatc
    17341 aggctcagcg ctttattcaa cgcacctctg atgtgatgaa agagctgaag tacatcgccg
    17401 aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg
    17461 atgtgcctgc gtttgaaccg ctgtttgccg actccgattg ttccgcaatg agtaacacct
    17521 ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg
    17581 cggcttacga tctcaaccgg gtatttttaa tcggcagtga taacctctgc atggccaatt
    17641 tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt gaaagctttg catgaacgca
    17701 tcaataaata tcgaaatgca ccacaagatg atagcggcag taacctctac tggatcagcg
    17761 aaggtccgcg ccctggcgtc gggtattttt acgcgttgac gccagtttat ctggcgaacc
    17821 ggttgcaggc gcttttgggt gtcgagcaga ccatccggat ggagaacttt ttcttaccgg
    17881 gtacgttgcc gatgggggtt accattcttg atgaaaatgg tcataccctg atttcgctta
    17941 ccggaccaga aagtaaaatt aagggcgatc ctcgctggat gcaggaacgc tcctggtttg
    18001 gctatacgga agggttccgg gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa
    18061 gcatcgtgta ttcggtgccg gttgataagg tgctggaacg cattcgcatg ttgatcctta
    18121 acgcaatttt gctgaatgtg cttgccggag ctgcattgtt tactctcgcg cggatgtacg
    18181 agcgacgtat tttcattccg gcggaaagcg acgccctgcg actggaagaa catgagcagt
    18241 tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg tattttgcgt accgctgatg
    18301 gcgtcaatat tttaagtaac gaactggcgc atacctatct caatatgctt acgcatgagg
    18361 accgccaacg actgacgcaa attatctgtg ggcagcaggt caattttgtt gatgtcctga
    18421 ccagcaacaa taccaatctg caaatcagct tcgtccattc gcgctatcgt aatgaaaacg
    18481 tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg
    18541 agatggcaca agcagcggaa caggcgagcc agtcaaaatc gatgttcctt gccaccgtca
    18601 gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa cctggatctg ttgcaaacca
    18661 aagagttacc gaaaggcgtc gatcggctgg tgacggcaat gaacaactct tccagcctgt
    18721 tgttgaaaat tatcagcgat attctcgatt tctcgaagat tgaatcggaa cagttgaaga
    18781 tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca catcaccgcc aactatttac
    18841 cgctggtggt acgcaagcag ttaggcttgt actgctttat tgaaccggat gtgccagtgg
    18901 ccttaaatgg cgacccgatg cgtttacagc aggtcatctc caacctgttg agtaacgcca
    18961 taaaattcac cgataccggc tgtatagttt tgcatgtccg cgctgatggc gattatctct
    19021 ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg
    19081 atcccttctt ccaggtcgga acgggcgtac agcgaaactt ccaggggacc ggtctgggtc
    19141 tggcgatttg tgaaaaactg atcagcatga tggacggcga tatctcggta gattcagaac
    19201 cgggaatggg cagccagttt accgtgcgta ttccgttgta cggcgctcag tacccgcaga
    19261 aaaaaggcgt ggaagggttg agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc
    19321 tctgtcagtt cctggaaacc agtttgcagc gcagcggcat cgtcgttaca acatacgaag
    19381 ggcaggaacc gactcccgaa gatgtgttaa ttactgacga ggtagtgagt aaaaaatggc
    19441 agggtagagc ggtagtgacc ttctgtcgtc gccatattgg tattccgctg gagaaagcgc
    19501 caggggagtg ggtacacagt gtggctgccc cgcatgagct accggcattg ttggcgcgta
    19561 tttatttgat cgagatggag agcgacgatc ctgctaacgc tctgccgtcg acggacaaag
    19621 cggtcagcga taatgacgat atgatgattc tggtcgtgga tgatcatccg attaaccgac
    19681 gtctgctggc agatcagttg ggatcgttgg gctatcaatg taaaaccgcg aatgatggtg
    19741 tcgatgcgct taatgtactt agcaagaatc atattgatat cgtgcttagc gacgtcaaca
    19801 tgccaaatat ggatggttac cgcttgacgc aacgcattcg tcagttggga ctgacgttgc
    19861 cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg
    19921 gtatggacag ctgcctgtcg aagccggtaa cgctggatgt gataaaacag acgctgacgg
    19981 tatatgccga gagggtcagg aaatcgcggg aatcgtagga cggataaggt gtttatgccg
    20041 catctggcat tcagtgcaaa tgccagatgc gacgctgacg cgtcttatct ggcctacttt
    20101 aatgtaatct gttgaaataa tgggaatcgt aggccggata aggcgtttac gctgcatccg
    20161 gcaatcggtg caaatgccag ataagacact aacgcgtctt atctggccta cagatgatta
    20221 gtctttatct gccggactta aggtcacaga agagagataa ttcagcaggg cgatatcgtt
    20281 ctcgacaccc agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg
    20341 gttcagcttt ttagcgatct cggtcaccag gaagccttcc gcaaacaggc gcagaacttc
    20401 actctctttt ggcgagagac gcttgtcacc gtaaccacca gcactgattt tttccaacag
    20461 gcgagaaacg ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg
    20521 cagatcggtc ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc
    20581 actaagaatc gccgggttgt tgttcatggt cagaacaatg atcgacaggc ttgggaaatg
    20641 gcgcttgatg tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag
    20701 atcggtaatc aacacatgcg catccagttt cggcaggttg ttgatcagtg ctgtagagtc
    20761 ttcaaattcg ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa
    20821 caagactatc ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac
    20881 cttgctacag caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg
    20941 gaacatcctt ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca
    21001 gatttagcat ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt
    21061 cactggttgc tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga
    21121 gcgcataata gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg
    21181 tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt
    21241 tgctcatatt gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat
    21301 catcgcttaa aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat
    21361 cataatcttg actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta
    21421 actggcgagt gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat
    21481 catccagtaa acgctcttct tcctcttcaa cttccgggtc agctgcgagc attttgatat
    21541 gcacagagta gcgtgtacca agcccatccc acgttttgat gtttaaatga ccgcccagtt
    21601 tacgtgccag ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt
    21661 tttgggtctg gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt
    21721 ctcccgtgtc cagaatgcgg aacgtcaggc ggtcttcgga ggactcatcc tgatcaacct
    21781 caagggtgat ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa
    21841 tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa
    21901 tcagcagttg cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa
    21961 ttaaatcctg cacggagaac agcaccgtct cacttttcca gctatcatcc gcaagcatgt
    22021 tcgctaactg tatttcatca accagccgca ccagcacatc tgcctgattc gccagttgtt
    22081 tgctttccgg ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca
    22141 gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg ctggtttttc tcatacagac
    22201 gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga
    22261 aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat
    22321 tgatcgtcgc ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat
    22381 tcaaatgcgg cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct
    22441 gatcgtgaac cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta
    22501 aaatgcgcaa ttcgttattg accgccgtgc tgggtagact ttctgtactg cggctggaga
    22561 aatggcggaa tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca
    22621 gtggcagcag aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga
    22681 ccaggcgcat atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt
    22741 ggatactgac gctatccgtc ccttctttct cattatcatt gtctcccgtt gccgtcgcgt
    22801 ctggctcaag gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggca
    22861 gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa
    22921 agtaatgacc gttctgccag gccaggcggc gcatgttaga aaaactttcg cgttcatcga
    22981 gggcgttggc ctgttgcaac atttctgcac gacgtgaatc aacaatgtca ctgacggtcg
    23041 attctttaaa tccggaggtg agatctttga ggggcagggt tgagatcagc accagactgt
    23101 tatcctgacc attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtgt
    23161 ccagataggt ggacatccgt tgagtcatct caagcgttga gttgtcgtga gagccaaaga
    23221 ttaacgcttc cgttttgcgg cgcggttttt ccagatagta gacatcctgt ttcaggcgcg
    23281 tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc ggcaatgttg tcgtagatct
    23341 gccaggtcac gtaacgccag gtatcgacgc gcttttggat agcgtgggta atgtcgacaa
    23401 tctggtaact tttatctttc agccaggcgt taacggcgct ttgtaccatt acccccatcg
    23461 tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg
    23521 aaaagcgggt cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac
    23581 ttcgcgaagt gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat
    23641 tgatgattct ctattctccc gtgatattta tcatcctgca ttgggggaaa tagatgtaca
    23701 agcgccattt tgctgtacaa ataacctaca aaaagcccaa caaaaataat tgcaaataaa
    23761 caaagattgc tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat
    23821 agtttttctg tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccgg
    23881 atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata
    23941 aataaagtta atgatgatag cgggagttat tctagttgcg agtgaaggtt ttgttttgac
    24001 attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa ttattattgc
    24061 ttgatgttac gtgcttattt cgccattccg caataatctt aaaaagttcc cttgcattta
    24121 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
    24181 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
    24241 cagtatgcag tggcataaaa aagcaaataa aggcatataa ctgagggtta ataacatgaa
    24301 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
    24361 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcctgca
    24421 ctatttctct gacaacaaag atgtagatgg cgaccagacc tacatgcgtc ttggcttcaa
    24481 aggtgaaact caggttactg accagctgac cggttacggc cagtgggaat atcagattca
    24541 gggcaacagc gctgaaaacg aaaacaactc ctggacccgt gtggcattcg caggtctgaa
    24601 attccaggat gtaggttctt tcgactacgg tcgtaactac ggcgttgttt acgacgtaac
    24661 ttcctggacc gacgtactgc cagaattcgg tggtgacacc tacggttctg acaacttcat
    24721 gcagcagcgt ggtaacggct tcgcgaccta ccgtaacact gacttcttcg gtctggttga
    24781 cggcctgaac tttgctgttc agtaccaggg taaaaacggt agcgtaagcg gcgaaggcat
    24841 gaccaacaac ggtcgtggtg ctctgcgtca gaacggcgac ggcgttggcg gatctatcac
    24901 ttatgattac gaaggcttcg gtatcggtgg tgcgatctcc agctccaaac gtactgatga
    24961 tcagaacagc ccgctgtaca tcggtaacgg cgaccgtgct gaaacctaca ccggtggtct
    25021 gaaatacgac gctaacaaca tctacctggc tgctcagtac acccagacct ataacgcaac
    25081 tcgcgtaggt tccctgggtt gggcgaacaa agcacagaac tttgaagctg ttgctcagta
    25141 ccagttcgac ttcggtctgc gtccgtctgt agcatacctg cagtctaaag gtaaaaacct
    25201 gggtaccatc gctggtcgta actacgacga cgaagatatc ctgaaatatg ttgatgttgg
    25261 tgctacctac tacttcaaca aaaacatgtc cacctacgtt gactacaaaa tcaacctgct
    25321 ggacgacaac cagttcactc gtgacgctgg catcaacact gataacatcg tagctctggg
    25381 tctggtttac cagttctaat ctcgattgat atcgaacaaa gggcctgcgg gccctttttt
    25441 cattgttttc agcgtacaaa ctcatttttt tggtgtactc ttgcgaccgt tcgcatgagg
    25501 ataatcacgt atggaaataa gctttacccg cgtggcactg ctggctgccg cgctcttctt
    25561 tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag ttaccgttct
    25621 tgaaggcaaa accatgggta ccttctggcg tgccagcatc ccgggcattg atgccaaacg
    25681 cagtgccgaa cttaaagaaa agattcagac ccagctggac gccgacgatc agctgctttc
    25741 gacctataaa aaagattcca cgctgatgcg ctttaacgac tcgcaaagtt tgtcgccgtg
    25801 gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg gcgcaaggac
    25861 cgatggtgcg atggatataa ccgtcgggcc gctggtgaat ctgtggggct ttggcccgga
    25921 acaacagccg gttcaaattc cgagccagga acagatcgat gcgatgaaag ccaaaaccgg
    25981 cttacagcac ctgacggtca tcaatcagtc gcatcagcaa tatctgcaaa aagacctgcc
    26041 ggatttatat gtcgatctct ccaccgtcgg tgaaggttat gcggcggatc acctggcacg
    26101 cttgatggag caggaaggta tttcacgtta tctggtgtca gttggtggtg ctctggatag
    26161 ccgtggtatg aacggtgaag gccagccgtg gcgggtagcg attcaaaaac caaccgataa
    26221 agaaaacgcg gttcaggccg tggtggatat caacggtcat ggcattagca cctctggcag
    26281 ctaccgtaac tattacgaac tggacggcaa acgtctttct catgttatcg atccgcaaac
    26341 cgggcgtcct atcgaacaca atctggtatc cgtgacggtg attgccccga cggcgctgga
    26401 agccgatgcc tgggatactg gcttaatggt actcgggccg gagaaagcca aagaggttgt
    26461 tcgccgggaa gggctggcgg tctatatgat caccaaagaa ggtgatagct ttaaaacctg
    26521 gatgtcgccg cagtttaaaa gcttccttat cagcgaaaaa aattaaagcg caagattgtt
    26581 ggtttttgcg tgatggtgac cgggcagact aaaggctatc cttaaccagg gagctgatta
    26641 tgaaaaaagc cacatgctta actgacgatc aatgctggca atctgtctta gcccgcgacc
    26701 cgaatgccga cggcgaattc gttttcgccg tgcgtaccac aggcatcttt tgccgtccgt
    26761 cttgccgcgc cagacatgct ttgcgggaaa acgtctcctt ctacgcaaat gccagcgagg
    26821 cactcgccgc tggctttcgc ccctgcaaac gttgtcagcc agaaaaagcc aatgcccagc
    26881 aacatcggtt ggataaaatc acccacgcgt gtcgactgct ggaacaggaa acgcctgtaa
    26941 cgctggaagc cttagccgac caggtggtga tgagtccatt tcatctacat cggttgttta
    27001 aagcgactac cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct cgccgtttgc
    27061 gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc ggattccccg
    27121 acagcagcag ttactaccgc aaagccgatg aaacgctggg catgacggct aaacaattcc
    27181 gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag ctgggtcgta
    27241 gcctggtggc agaaagcgag cgggggattt gcgcgatatt gctgggcgat gatgacgcga
    27301 cactaatcag cgagttgcag cagatgtttc ccgctgccga caacgcgcct gccgatctga
    27361 tgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat acgccgctga
    27421 cgttaccgct ggacattcgc ggcactgctt ttcagcaaca agtctggcag gcactgcgca
    27481 cgataccttg cggtgaaacc gtcagttatc agcaactggc taacgccatc ggcaaaccga
    27541 aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc ataataccct
    27601 gtcatcgggt ggtccgtggt gatggcacac tttccggtta ccgctggggc gtgtcgcgta
    27661 aagcgcaact gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg atctgtttgc
    27721 cgatgctgaa ccgtggcaag agccactggc ggctggtgcg gtaattttac ggcgttttgc
    27781 ttttaatgct gcggagcaac taatccgcga tattaatgac gttgccagcc agtcgccgtt
    27841 tcgccagatg gtcacccccg ggggatatac catgtcggtg gcgatgacca actgtgggca
    27901 tctgggctgg acgagccatc ggcaaggtta tctctattcg cccattgatc cgcaaacaaa
    27961 taaaccgtgg cccgccatgc cacagagttt tcataattta tgtcaacgtg cggctacggc
    28021 ggcgggctat ccagatttcc agccagatgc ttgtcttatc aaccgctacg ctcctggcgc
    28081 gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccaa ttgtttctgt
    28141 ttctctgggc ttacctgcga tttttcaatt tggtggcctg aaacgtaatg atccgctcaa
    28201 acgtttgttg ttggaacatg gcgatgtggt ggtgtggggc ggtgagtcgc ggctgtttta
    28261 tcacggtatt caaccgttga aagcggggtt tcatccactc accaccgact gccgctacaa
    28321 cctgacattc cgtcaggcag gtaaaaaaga ataaaaataa gaattattat tgctgtgcgc
    28381 gcgaagatgt ttaaactgcg ggctgtaatt cattgtccgg gttttctgca tggaacttct
    28441 tgtacttgtc tggcggcagt atcgctggcc atttatcagt gtgatggcgc taagcctcgc
    28501 cagtgcggca ttaggcattg gcttaattgc ttttatcaat cagcgcctta tcgaaacggc
    28561 ggataccagt ctgctggtat tgccggagtt tctgggatta ttgctgctgt tgatggcagt
    28621 cactctcgga tcgcaactgg cgctcaccac tttggggcat cacttcgttt accgactgcg
    28681 tagtgaattt atcaagcgga ttctggatac caacgtcgag cgcattgaac aactcggtag
    28741 cgcctcgttg ctggcgggat taaccagcga tgtgcgcaat atcaccattg cttttgtgcg
    28801 tcttccggaa ctggtgcagg ggatcattct cactatcggt tccgcggcgt atctgtggat
    28861 gctgtcgggc aaaatgttgc tggtaacggc tatctggatg gcgatcacca tctggggtgg
    28921 ttttgtactg gtggcgcggg tgtacaaaca tatggcgacc ctgcgtgaaa ccgaagacag
    28981 gctgtatacg gattttcaaa ctgtactcga agggcgcaaa gagctgactc tgaaccggga
    29041 acgcgccgag tatgtgttta acaacctcta cattcctgat gcgcaagagt atcgccacca
    29101 tattatccgc gcagacacct tccatcttag cgccgtgaac tggtcaaaca tcatgatgtt
    29161 gggcgcaatc ggcctggtgt tctggatggc gaacagtctc ggttgggctg ataccaacgt
    29221 tgccgcgacc tattcgttga cgcttttatt cctgcgtacg ccgctgcttt cggcagttgg
    29281 cgcattgccg acgctgctga cggcgcaggt ggcgtttaac aagctgaaca aattcgcgct
    29341 cgcgcctttc aaagcagagt ttccgcgccc gcaggcgttt cccaactggc aaacgctgga
    29401 gctgcgtaac gtgacgtttg cttatcagga taacgcgttt tccgttggtc cgattaatct
    29461 caccatcaaa cgtggcgagc tgctgtttct gattggcggc aacggtagcg gaaaatcgac
    29521 gctggcgatg ttgttgacgg gcttgtatca accacaaagc ggcgaaatct tgctggatgg
    29581 caaacctgtc agcggcgaac aaccggaaga ttatcgcaaa ctgttttcgg cagtgtttac
    29641 cgatgtctgg ctgtttgatc aactgctggg gccggagggt aaacccgcta acccgcaact
    29701 ggttgagaag tggctggcgc agctgaaaat ggctcataag cttgagttaa gcaacgggcg
    29761 tattgttaac ctgaagttat caaaagggca gaaaaaacgc gtggcgctgt tgctggcgct
    29821 ggcagaagaa cgcgatatta tcctgctgga tgaatgggcg gcggatcagg atccacactt
    29881 ccgtcgtgag ttttatcagg tgttgctgcc gctgatgcag gagatgggta aaactatttt
    29941 cgccatcagt catgatgatc attactttat ccacgccgac cgcctgctgg aaatgcgcaa
    30001 cgggcaactt agcgagctga cgggcgaaga gcgcgatgct gcttcgcgtg atgccgttgc
    30061 ccggacggca taatattacg acgctgacct ggcggactgc tgccgccagg tcaatatgct
    30121 aaaaatccct tcatttctaa taaccctata attaatcaac gaaattataa tgtttctaaa
    30181 attagaatat aatttataaa cattatttaa atgttgttac ttaagtgtta ccgttgatgc
    30241 cgcgcaaatc tcacctgcaa taaagcgact aaaagtaagg cattaacaag atgaaaaaag
    30301 tgactgccat gctcttctcg atggccgtgg ggcttaatgc cgtttcgatg gcggcaaaag
    30361 cgaaagcgtc cgaggagcag gaaactgatg tactgttgat tggcggcggc attatgagcg
    30421 ccacgttggg gacctattta cgcgagctgg agcctgaatg gtcgatgacc atggtggagc
    30481 gcctggaggg tgtcgcgcag gagagttcga acggctggaa taacgccgga accgggcatt
    30541 ctgcactgat ggaactgaac tacaccccgc aaaacgccga tggcagcatc agtattgaaa
    30601 aagcagtcgc cattaacgaa gcatttcaga tttcccgcca gttctgggcg caccaggtcg
    30661 agcgcggcgt gctgcgtact ccgcgttcat ttatcaatac cgttccgcat atgagctttg
    30721 tctggggcga ggataacgtt aatttcctgc gcgcccgtta cgccgcgttg caacaaagct
    30781 cgctgtttcg cggtatgcgt tactctgaag atcacgcgca gatcaaagag tgggcacctt
    30841 tagtgatgga agggcgcgat ccgcaacaga aagtggcagc cacgcgtacg gaaattggta
    30901 ccgatgtgaa ctacggcgag atcacccgcc agttaattgc ttccttgcag aagaaatcta
    30961 acttctcgct gcaactcagc agcgaagtcc gcgccctaaa gcgtaatgac gataacacct
    31021 ggaccgttac cgttgccgat ctaaaaaatg gcactgcaca gaacattcgt gccaaatttg
    31081 tctttatcgg cgcgggcggt gcggcgctga agctattaca ggaatcgggg attccggaag
    31141 cgaaagacta cgccggtttc ccggtgggcg gacagttcct tgtttcggaa aacccggacg
    31201 tggttaatca ccatctggcg aaggtttacg gtaaagcatc cgttggcgca ccaccgatgt
    31261 cggttccgca tatcgatacc cgcgttctgg acggtaaacg cgtagtgctg tttgggccat
    31321 ttgccacctt ctcaaccaaa ttcctcaaaa acggttcatt gtgggatctg atgagttcca
    31381 ccaccacctc taacgtgatg ccgatgatgc acgtcgggct ggataatttc gatctggtga
    31441 aatatctggt gagtcaggtg atgttgagtg aagaggatcg ttttgaagcg ttgaaagagt
    31501 actatccgca agcgaaaaaa gaggactggc gtttgtggca agcggggcag cgcgtgcaga
    31561 ttatcaagcg tgatgccgat aaaggtggtg tactgcgtct gggtactgaa gtggtcagtg
    31621 accagcaagg gaccattgcc gcgctgctgg gggcatcgcc aggggcgtca accgccgcgc
    31681 cgattatgtt ggatctgctg gaaaaagtat ttggcgatcg cgtttccagc ccgcaatggc
    31741 aggctacgtt gaaagcgatc gttccgtctt atggacgcaa gctgaacggt gatgtagcgg
    31801 caacagaacg cgagttgcag tacaccagcg aagtgctggg gctgaaatac gacaagccgc
    31861 aggcagcaga tagtacgcca aaaccgcagt tgaaaccgca acccgttcaa aaagaagtgg
    31921 cggatattgc gttgtaatga tgtgccacat ccggcatggt atgccggatg tggcgtatgc
    31981 tgataagacg cgccagcgtc gcatcaggca ccggctcggg cgttagatgt cggatgcggc
    32041 gtgaccgcct tattcggcct acggggtgcc gcacttcaca gcattttagc gaactaccgc
    32101 gttgtcaatt ttctcttccg ccttccagac gcggtacttc acatctacat tgtctggcgt
    32161 atacaccacg atcggcagct tgctgttgta acgcagcatt ccagcatcgc ccagatacgc
    32221 ggtgacaaat ttcttctctt tcttgccatc cgggcaggcc atcatcgtgg aaaccggcga
    32281 gctaacttta tcaaagacat aataatcata gccccagcct tccagcgttt tgttttccag
    32341 cttcccgccg agacgatgca aattgcaatc gacttccagc gtctgaccga ttaacagttc
    32401 tactttcagg gtagattcat cttcttgcgg ggttaactga atcacctgac gcttcatccc
    32461 tttttcagct tgtggataag gcgcgatttt ttccagtggc tggacgcttt ctgccgccca
    32521 ggcggaagtg gtagcgaaag cggcaaacaa tactgcaggt agaatggtct tcattttttt
    32581 gctttccttc ttcatttata ggttttcacc gatgatgtcg ctgaaggtta acatattcat
    32641 cagatttatt aatttgcttt atgaataact taaag
//
LOCUS       DAZFYC010000045        32524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFYC010000045 DAZFYC010000000
VERSION     DAZFYC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.045"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            155..454
                     /locus_tag="ACHKW5_002366"
     CDS             155..454
                     /locus_tag="ACHKW5_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511751.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILALFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(501..1388)
                     /gene="rarD"
                     /locus_tag="ACHKW5_002367"
     CDS             complement(501..1388)
                     /gene="rarD"
                     /locus_tag="ACHKW5_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO7511752.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1440..1907)
                     /gene="yigI"
                     /locus_tag="ACHKW5_002368"
     CDS             complement(1440..1907)
                     /gene="yigI"
                     /locus_tag="ACHKW5_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO7511753.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2072..2941
                     /gene="pldA"
                     /locus_tag="ACHKW5_002369"
     CDS             2072..2941
                     /gene="pldA"
                     /locus_tag="ACHKW5_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO7511754.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3074..4903
                     /gene="recQ"
                     /locus_tag="ACHKW5_002370"
     CDS             3074..4903
                     /gene="recQ"
                     /locus_tag="ACHKW5_002370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO7511755.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFSEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4967..5587
                     /gene="rhtC"
                     /locus_tag="ACHKW5_002371"
     CDS             4967..5587
                     /gene="rhtC"
                     /locus_tag="ACHKW5_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO7511756.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5649..6269)
                     /gene="rhtB"
                     /locus_tag="ACHKW5_002372"
     CDS             complement(5649..6269)
                     /gene="rhtB"
                     /locus_tag="ACHKW5_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO7511757.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6381..7403
                     /gene="pldB"
                     /locus_tag="ACHKW5_002373"
     CDS             6381..7403
                     /gene="pldB"
                     /locus_tag="ACHKW5_002373"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO7511758.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7411..8211
                     /gene="yigL"
                     /locus_tag="ACHKW5_002374"
     CDS             7411..8211
                     /gene="yigL"
                     /locus_tag="ACHKW5_002374"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO7511759.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8287..9186
                     /gene="bioP"
                     /locus_tag="ACHKW5_002375"
     CDS             8287..9186
                     /gene="bioP"
                     /locus_tag="ACHKW5_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO7511760.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9074..10027)
                     /gene="metR"
                     /locus_tag="ACHKW5_002376"
     CDS             complement(9074..10027)
                     /gene="metR"
                     /locus_tag="ACHKW5_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO7511761.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10150..12411
                     /gene="metE"
                     /locus_tag="ACHKW5_002377"
     CDS             10150..12411
                     /gene="metE"
                     /locus_tag="ACHKW5_002377"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO7511762.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12450..13265)
                     /gene="ysgA"
                     /locus_tag="ACHKW5_002378"
     CDS             complement(12450..13265)
                     /gene="ysgA"
                     /locus_tag="ACHKW5_002378"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO7511763.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13527..14288
                     /gene="udp"
                     /locus_tag="ACHKW5_002379"
     CDS             13527..14288
                     /gene="udp"
                     /locus_tag="ACHKW5_002379"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO7511764.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14429..15856
                     /gene="rmuC"
                     /locus_tag="ACHKW5_002380"
     CDS             14429..15856
                     /gene="rmuC"
                     /locus_tag="ACHKW5_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO7511765.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15951..16706
                     /gene="ubiE"
                     /locus_tag="ACHKW5_002381"
     CDS             15951..16706
                     /gene="ubiE"
                     /locus_tag="ACHKW5_002381"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO7511766.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16720..17325
                     /gene="ubiJ"
                     /locus_tag="ACHKW5_002382"
     CDS             16720..17325
                     /gene="ubiJ"
                     /locus_tag="ACHKW5_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO7511767.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17322..18962
                     /gene="ubiB"
                     /locus_tag="ACHKW5_002383"
     CDS             17322..18962
                     /gene="ubiB"
                     /locus_tag="ACHKW5_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO7511768.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19041..19310
                     /gene="tatA"
                     /locus_tag="ACHKW5_002384"
     CDS             19041..19310
                     /gene="tatA"
                     /locus_tag="ACHKW5_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO7511769.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19314..19829
                     /gene="tatB"
                     /locus_tag="ACHKW5_002385"
     CDS             19314..19829
                     /gene="tatB"
                     /locus_tag="ACHKW5_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO7511770.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19832..20608
                     /gene="tatC"
                     /locus_tag="ACHKW5_002386"
     CDS             19832..20608
                     /gene="tatC"
                     /locus_tag="ACHKW5_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO7511771.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20650..21432
                     /gene="tatD"
                     /locus_tag="ACHKW5_002387"
     CDS             20650..21432
                     /gene="tatD"
                     /locus_tag="ACHKW5_002387"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO7511772.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21429..21917)
                     /gene="rfaH"
                     /locus_tag="ACHKW5_002388"
     CDS             complement(21429..21917)
                     /gene="rfaH"
                     /locus_tag="ACHKW5_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO7511773.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22084..23577
                     /gene="ubiD"
                     /locus_tag="ACHKW5_002389"
     CDS             22084..23577
                     /gene="ubiD"
                     /locus_tag="ACHKW5_002389"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO7511774.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23623..24324
                     /gene="fre"
                     /locus_tag="ACHKW5_002390"
     CDS             23623..24324
                     /gene="fre"
                     /locus_tag="ACHKW5_002390"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO7511775.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24516..25679)
                     /gene="fadA"
                     /locus_tag="ACHKW5_002391"
     CDS             complement(24516..25679)
                     /gene="fadA"
                     /locus_tag="ACHKW5_002391"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO7511776.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25689..27878)
                     /gene="fadB"
                     /locus_tag="ACHKW5_002392"
     CDS             complement(25689..27878)
                     /gene="fadB"
                     /locus_tag="ACHKW5_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO7511777.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAANGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28068..29399
                     /gene="pepQ"
                     /locus_tag="ACHKW5_002393"
     CDS             28068..29399
                     /gene="pepQ"
                     /locus_tag="ACHKW5_002393"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO7511778.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29399..30013
                     /gene="yigZ"
                     /locus_tag="ACHKW5_002394"
     CDS             29399..30013
                     /gene="yigZ"
                     /locus_tag="ACHKW5_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO7511779.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30052..31503
                     /gene="trkH"
                     /locus_tag="ACHKW5_002395"
     CDS             30052..31503
                     /gene="trkH"
                     /locus_tag="ACHKW5_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO7511780.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31515..32060
                     /gene="hemG"
                     /locus_tag="ACHKW5_002396"
     CDS             31515..32060
                     /gene="hemG"
                     /locus_tag="ACHKW5_002396"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO7511781.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDHCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            32436..>32524
                     /locus_tag="ACHKW5_002397"
     rRNA            32436..>32524
                     /locus_tag="ACHKW5_002397"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7595 a         8228 c         8865 g         7836 t
ORIGIN      
        1 ttgcatatat atgcaatata gcagtctttg cattgataac tatacagtag taatttagag
       61 tgtgtaagtt tttattgatg gggtatattt taattaaata taattgaaat gtatttttat
      121 tctataaaca atatcttggg tgaatggatt ctatatggga aatggtaact tttggcagtt
      181 gttcttgggt aaagaacctt acagcaaaaa atctgatttt gttaaggcat ggttattgtg
      241 gtgggctatt accatccttg ctctatttat tttaagcctg actacgacat taaatttaag
      301 aagtataggt atagggttaa gttttgggtt ttgtcaaatc cttggtagat atacgatgtc
      361 tctaggatgg agtaagtact ggggagtttt gggggtattt cctgtaacaa atattgttct
      421 ttttcttatc ttaatttttt tcaaaaaaag ataaataagt cagtgggtag gctctttaaa
      481 cggcctaccc atgttaattc ttaacttttt ctacgctgcg tataaatcgc atccatcaca
      541 aaaattgcca gcgccaccca aataaaggcg aaagtcacca tcttatcggc acccggtttt
      601 tcaccataaa acgtcacagc cagcaggaac atcagcgtcg ggccaatgta ctggaaaaag
      661 cctaacgttg agagacgcaa gcgcgtggca gcggcggtaa aacacaacag cggtacggta
      721 gtgacaatac cggcggcgat cagcagtaaa ttcagcgaca tcgggttttg ccccatatgg
      781 ctggttgagc tgtcggcaat agcaaacagg taaattgccg ccacgggcag cagccacatg
      841 gtttcgatta acatgccggt ttgcgcttca acggcaatct tcttgcgtac cagaccgtag
      901 aaggcaaaac taaatgccag tcccagcgcg ataataggta gcgaaccaaa agtccacagc
      961 tggactaaca cgccacatat cgccagaatc accgccagcc attgcatccg gcggaatcgc
     1021 tcgccgagga aaatcatccc cagcacaatg ttcaccagcg ggttaataaa gtaaccaagg
     1081 ctcgcttcca gcatatggtg attgttcacc gcccagataa acagtagcca gttgccacca
     1141 atcagcacgg cagagactgc cagcataaaa attttctgtg gcgtctgaat cagcgttttt
     1201 aaataggacc actggcggca aatgctcatc agcaccacca taaagaaaaa cgaccagatc
     1261 acgcgatgcg tcaggatttc atcggcaggc acgtagtaaa tcaacttgaa gtacgctggc
     1321 gctatacccc aaataaaata agcggcaaga gcgagtaata cgccctgccg cgtttgtttt
     1381 gcatccatcg ggaatactca tttttaattg gtaacagcag tttacctgct tttatgtctt
     1441 caacctacca tataggtggc ggtggcactg gcaatataaa gctgttcttc attgtgtaat
     1501 tcaacgcggg cgacggcgac tttattgcct gcacgcaaca ggctactggt agcagtaaaa
     1561 cgctcgcccc tgcctgggcg cagataatca acgcgaagat caatggtccc catccgcgat
     1621 agccgctggc gtagttcatc ttcactgatg gtttcgtggc gggttaaggt acttcccacg
     1681 cacaccagac cggcggcgac atccagcgcc gacgcaatga ccccgccgtg caaaatgctt
     1741 tgcgcccagt tgcccaccat cattggctga tttttaaagg ccagctgtgc gaactctttt
     1801 tcgtaacgct ccagttccat ccccaatgcg cggttaaatg gcatgtgata aacaaacatc
     1861 tcacccacta atttcagggc ttgttcagcg gtcagtacgg cagacatatc atccttacac
     1921 ttcattggtt aatgaaatgt tgattttatg cttctttgtt gttggtttct actttaggaa
     1981 gggataacta acggctatgg agttaagtat gtaaaatagc ccgcagaaaa atattcacct
     2041 tatcaataat tcgttacgga gaacacgacc gatgcggact ctgcagggct ggttgttgcc
     2101 ggtgtttatg ttgcctatgg cagtatatgc acaagaggca acggtgaaag aggtgcatga
     2161 cgcgccagcg gtgcgtggca gtattatcgc caatatgctg caggagcatg acaatccgtt
     2221 cacgctctat ccttatgaca ccaactacct catttacacc caaaccagcg atctgaataa
     2281 agaagcgatt gccagttacg actgggcgga aaatgcgcgt aaggatgaag taaagtttca
     2341 gttgagcctg gcatttccgc tgtggcgtgg gattttaggc ccgaactcgg tgttgggtgc
     2401 gtcttatacg caaaaatcct ggtggcaact gtccaatagc gaagagtctt caccgtttcg
     2461 tgaaaccaac tacgaaccgc aattgttcct cggttttgcc accgattacc gttttgcagg
     2521 ttggacgctg cgcgatgtgg agatggggta taaccacgac tctaacgggc gttccgaccc
     2581 gacctcccgc agttggaacc gcctttatac tcgcctgatg gcagaaaacg gtaactggct
     2641 ggtagaagtg aagccgtggt atgtggtggg taatactgac gataacccgg atatcaccaa
     2701 atatatgggt tactaccagc ttaaaatcgg ctatcacctc ggtgatgcgg tgctcagtgc
     2761 aaaaggacag tacaactgga acaccggcta cggcggcgcg gagttaggct taagttaccc
     2821 gatcaccaaa catgtgcgcc tttatactca ggtttacagc ggctatggcg aatcgctcat
     2881 cgactataac ttcaaccaga cccgtgtcgg tgtgggggtt atgctaaacg atttgttttg
     2941 atgaacggtt gagtggttgg caaatctgga atccagcatc caggattacc ctctcagaga
     3001 ctaaaagcat tgcagtttct cgcgcaggcg ctgaaaatag cgcctgtttt tatttcaggc
     3061 aatcggggtg aatgtggcgc aggcggaagt gttgaatctg gagtccggag ctaaacaggt
     3121 tttacaagaa acctttggct accaacagtt tcgccccggc caggaagaaa ttatcgacac
     3181 tgtgctttcc ggtcgcgatt gcctggtcgt catgcccacc ggtggcggaa aatccctttg
     3241 ctatcaaatt cctgccttat tgctaaacgg ccttaccgtg gttgtttcac cgctgatttc
     3301 gttgatgaaa gatcaggtgg atcaactgca agccaacggc gtggcggcgg cgtgccttaa
     3361 ctcgacgcaa acccgcgaac agcaacttga agtgatgaca ggctgccgca ccgggcaaat
     3421 tcgtctgctt tatatcgccc cggaacgcct gatgctggat aactttcttg agcatctggc
     3481 gcactggaat ccggtgttat tagccgttga tgaagcgcac tgtatctccc aatggggcca
     3541 cgatttccgc ccggaatatg ccgcgctcgg tcagttgcgc cagcggttcc cgacgctgcc
     3601 gtttatggcg ctgaccgcca cagccgacga caccacgcgc caggatatcg tgcgcctgct
     3661 ggggctgaac gatccgctga ttcaaatcag cagttttgac cgtccgaata ttcgctacat
     3721 gctgatggag aagttcaaac cgctcgatca gttgatgcgc tacgtgcagg aacagcgcgg
     3781 taagtccggc attatctact gcaacagccg ggcgaaagta gaagacaccg ctgcgcgcct
     3841 gcaaagcaag ggtattagcg cggcggccta tcatgccggg ctggaaaata atgtccgcgc
     3901 cgacgtgcag gagaaattcc agcgcgatga cctgcaaatt gtggtggcga cggtggcgtt
     3961 cggcatgggc atcaataaac caaacgttcg cttcgtggtc cactttgata ttccgcgcaa
     4021 tatcgaatcc tattatcagg aaaccggtcg cgccgggcgt gatggcctgc ccgcggaagc
     4081 gatgctgttt tacgatccgg ccgatatggc gtggctgcgc cgttgtctgg aagagaagcc
     4141 gcaggggcag ttgcaggata tcgaacgtca taaactcaat gcgatgggcg cttttgccga
     4201 agcgcaaact tgccgtcgtc tggtactgct gaactatttt agcgaagggc gtcaggagcc
     4261 gtgcgggaac tgcgatatct gcctcgatcc gccgaaacag tacgacggtt caaccgatgc
     4321 tcagattgcc ctttccacca ttggtcgtgt gaatcagcgt tttggtatgg gctatgtggt
     4381 ggaagtgatt cgcggcgcta ataaccagcg tatccgcgac tatggtcatg acaaactaaa
     4441 agtctacggt atgggccgtg ataaaagtca tgaacattgg gtgagcgtga tccgccagtt
     4501 gattcacctc ggcctggtga cgcaaaatat tgcccagcat tctgccctcc aactgacaga
     4561 ggccgcgcgt ccggtgctgc gcggcgaatc ctctttgcaa cttgccgtgc cgcgtatcgt
     4621 ggcgctcaaa ccgaaagcga tgcagaaatc gttcggcggc aactatgatc gcaaactgtt
     4681 cgccaaatta cgcaaactgc gtaaatcgat tgccgatgaa agcaatgtcc cgccgtacgt
     4741 ggtgtttaac gacgcaacgt tgattgagat ggctgaacag atgccgatca ccgccagcga
     4801 aatgctcagc gttaacggcg ttgggatgcg caagctggaa cgctttggta aaccgtttat
     4861 ggcgcttatc cgcgcgcatg ttgacggcga cgacgaagag tagtcagcgg cataaaaaag
     4921 tgccaggatg aagactccgt aaacgtttcc cccacgagtc aaatgtatgt tgatgttatt
     4981 tctcaccgtc gccatggtgc atattgtggc gcttatgagc cccggtcccg atttcttttt
     5041 tgtctctcag accgctgtca gtcgttcccg taaagaagcg atgatgggcg tgctgggcat
     5101 tacctgcggt gtaatggttt gggctgggat tgcgttgctt ggcctgcatt tgattatcga
     5161 aaaaatggcc tggctgcata cgctgattat ggttggcggt ggcctgtatc tctgctggat
     5221 gggttaccag atgctacgcg gtgcactgaa aaaagaggcg gtttctgcac ctgcgccaca
     5281 ggtcgagctg gcgaaaagtg ggcgcagttt cctgaaaggt ttactgacca atctcgctaa
     5341 tccgaaagcg attatctact ttggctcggt gttctcattg tttgtcggtg ataacgttgg
     5401 cactaccgcg cgctggggca tttttgcgct gatcattgtc gaaacgctgg cgtggtttac
     5461 cgtcgttgcc agcctgtttg ccctgccgca aatgcgccgt ggttatcaac gtctggcgaa
     5521 gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt tgattatttc
     5581 gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc cgcatccgac
     5641 attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat aaacaacgag
     5701 ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag agcaatccgt
     5761 tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt ggtgacgccg
     5821 agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg aaatagcgcc
     5881 gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg ctggaacaaa
     5941 tgtcgacgcg attgagtaga ggccagcgat ttaaggtcaa ttgcaccagc ggcgcgccac
     6001 tgctggattc ccagccaaat caagtaagcc gcgcctgccc acttcaacac ttcaaacgca
     6061 atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat atgaatcgcc
     6121 agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg gttgagcgag
     6181 gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat cgatgtcagc
     6241 aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta tttttgtgcc
     6301 acaatacgct actgtcgcag cgttgtgtca ggcacgctaa aaaaaaacga ttttacgtgg
     6361 tttaagaggc agattacccg atgtttcagc agcaaaaaga ctgggaaaca agagaaaacg
     6421 cgtttgctgc ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt gatgaagcag
     6481 agtttactgg tgtggatgac attccggtgc gctttgtccg ttttcgcgca cagcaccatg
     6541 accgggtggt agtcatctgc ccggggcgta ttgagagcta cgtaaaatat gcggaactgg
     6601 cctacgattt gttccatttg gggtttgatg tcttaatcat cgaccatcgc gggcagggac
     6661 gttccggtcg cctgttagcc gatccgcatc tcgggcatgt taatcgcttt aatgattatg
     6721 ttgatgatct ggcggcattc tggcaacagg aggtccagcc cggtccgtgg cgtaaacgct
     6781 atatactggc acattcgatg ggcggtgcga tctccacatt atttctgcaa cgccatccag
     6841 gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt cgtatgccgt
     6901 catttatggc acggcagatc ctcaactggg ccgaagcgca tccacgtttc cgtgatggct
     6961 atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta ctgacccaca
     7021 gcagacagcg atatcgacgt aacttacgct tctatgctga tgacccaacg attcgcgtcg
     7081 gtgggccgac ctaccattgg gtacgcgaaa gtattctggc tggcgaacag gtgttagccg
     7141 gtgcgggtga tgacgccacg ccaacgcttc tcttgcaggc tgaagaggaa cgcgtggtgg
     7201 ataaccgcat gcatgaccgt ttttgtgaac tccgcaccgc cgcgggccat cctgtcgaag
     7261 gaggacggcc gttggtaatt aaaggtgctt accatgagat cctttttgaa aaggacgcaa
     7321 tgcgctcagt cgcgctccac gccatcgttg attttttcaa caggcataac tcacccagcg
     7381 gaaaccgctc tacagaggtt taaatttctt atgtaccagg ttgttgcgtc tgatttagat
     7441 ggcacgttac tttctcccga ccatacgtta tccccttacg ccaaagaaac tctgaagctg
     7501 ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt tgatgtgggg
     7561 caaattcgcg ataatctgga gattaagtct tacatgatta cctccaatgg tgcgcgcgtt
     7621 cacgatctgg atggtaatct gatttttgct cataacctgg atcgcgacat tgccagcgat
     7681 ctgtttggcg tagtcaacga caatccggac atcattacta acgtttatcg cgacgacgaa
     7741 tggtttatga atcgccatcg cccggaagag atgcgctttt ttaaagaagc ggtgttcaaa
     7801 tatgcgctgt atgagcctgg attactggag ccggaaggcg tcagcaaagt gttcttcacc
     7861 tgcgattccc atgaacaact gctgccgctg gagcaggcga ttaacgctcg ttggggagat
     7921 cgcgtcaacg tcagtttctc taccttaacc tgtctggaag tgatggcggg cggcgtttca
     7981 aaaggccatg cgctggaagc ggtggcgaag aaactgggct acagcctgaa ggattgtatt
     8041 gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga tggcggggaa aggctgcatt
     8101 atgggcagtg cgcaccagcg tctgaaagac cttcatcccg agctggaagt gattggtact
     8161 aatgccgacg acgcggtgcc gcattatctg cgtaaactct atttatcgta atcgttcttt
     8221 atttggtcag ttgtcaacct gatacttcgc tacaatggat actcgttaat caaagagttt
     8281 tccattgtgg cgctacttat catcaccacg attctgtggg ccttctcctt tagcttttat
     8341 ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg cgttggcctg
     8401 gcggcactcg tttttctgcc gtttctgcgt acccgtggca atagcctgaa aacagtcggc
     8461 ctgtatatgc tggtgggcgc gatgcagctt ggcgtgatgt atatgctgag tttccgcgct
     8521 tatctctatc tgacggtttc cgaactgctg ctgttcaccg tactgacgcc gctctacatc
     8581 acgctgattt atgacatcat gagtaagcgc cgtctgcgct ggggatatgc ttttagtgcc
     8641 ttgttggcgg tgattggtgc cgggattatt cgctatgatc aggtcaccga ccatttctgg
     8701 actggcttgt tgctggtgca actctccaat atcacttttg ccatcggcat ggtgggttac
     8761 aaacgcctga tggaaactcg cccgatgccg cagcataacg cctttgcgtg gttctatctt
     8821 ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca gaaaatgccg
     8881 caaaccacgt tgcaatgggg cattctggtg tttcttggcg tggtggcttc cgggattggc
     8941 tactttatgt ggaactacgg cgcgacgcag gtggacgccg gaacgctggg aattatgaat
     9001 aatatgcacg ttccggcagg gctgctggta aacctggcta tctggcacca acagccgcac
     9061 tggccaacgt ttattacagg cgcgctggtg atcctggcct cactgtgggt gcatcgtaag
     9121 tgggtcgctc cgcgctcttc acaaacggca gatgatcgca ggcgtgattg cgcgctgagc
     9181 gaataaacgc ttccgtaact ggctgacgct gctcgccatc gcgcacggcg gcgtacagtc
     9241 ggctccacaa gccttcgccc agggttttgg tcaccaccag accctggcgc tcaaaactct
     9301 ctactaccca atgcggtagc gcggcaatac ccatccgcgc ggcaaccatc tgaatcaaca
     9361 ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc tgccggctga agaaaatgcc
     9421 gccagacatc cagtcgacta cgctgcaccg gataaatcaa cagcgtctcg ctggcgagat
     9481 cttccggtgt aattcgcgtt ttcgccgcca gtggatggtc aggtgctaac accagacgca
     9541 cttcatagtc gaacatcggc gaataatgca ggccactgcg cggcaggata tcggaggtca
     9601 tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg gtcaaacgtc acgcccgatt
     9661 taaaatccat ctctacctgc ggccagttct tatggaaatt ttctaacgcg ggcgtcagcc
     9721 actgaataca gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc ggttcattgc
     9781 aggcttgcag tgcctggcta atttgcggca gcacctggtt tgccagttgc aacaggattt
     9841 ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac aaatagccgg aagccaaggc
     9901 gttgttccag atcgctaaac tggtgagaca gggcggattg cgtctgatgc aacgtcgccg
     9961 cagcggctgc gagagagccg cagttccgca acgcttgtag cgttttcagg tgttttactt
    10021 cgatcatgaa agtccttcac ttcggcatga ataatttgcg cttgaggaat atacagtaac
    10081 cgccaattat ggatgtgtaa acatctggac ggctaaatac taaaaattcc ataaggggca
    10141 tatcatataa tgacaattct taatcacacc ctcggtttcc ctcgcgttgg cctgcgtcgc
    10201 gagctgaaaa aagcgcaaga aagttattgg gcggggaact ccacgcgtga agaactgctg
    10261 acagtagggc gtgaactgcg tgctcgtcac tgggatcaac aaaagcaagc gggtatcgac
    10321 ctgctgccgg tgggcgattt tgcctggtac gatcatgttc tgaccaccag tctgctgctg
    10381 ggtaatgttc cgccacgtca tcagaacaaa gacggttcgg tagatatcga caccctgttc
    10441 cgtattggtc gtggacgtgc gccgactggc gaacctgcgg cggcagcgga aatgaccaaa
    10501 tggtttaaca ccaactatca ctacatggtg ccggaattcg ttaaaggcca acagttcaaa
    10561 ctgacctgga cgcagctgct ggaggaagtg gacgaggcgc tggcgctggg ccacaacgtt
    10621 aagcctgtgc tgctggggcc ggttacttac ctgtggctgg ggaaagtgaa aggtgaacag
    10681 tttgaccgcc ttagcctgct gaacgacatt ctgccggttt atcagcaagt gctggcagaa
    10741 ctggcgaaac gcggcatcga gtgggtacag attgatgaac ccgcgctggt actggaacta
    10801 ccacaggcgt ggctggacgc atacaaaccc gcttacgacg cgctccaggg acaggtgaaa
    10861 ctgctgctga ccacctattt tgaaggcgta acgccaaatc tcgacacgat tactgcgctg
    10921 cctgttcagg gtctgcatgt tgacctcgta catggtaaag atgacgttgc tgaactgcac
    10981 aagcgcctgc cttctgactg gttgctgtct gcgggtctga tcaatggtcg taacgtctgg
    11041 cgcgccgatc ttaccgagaa atatgcgcaa attaaggaca ttgtcggcaa acgtgatttg
    11101 tgggtggcat cttcctgctc gttgctgcac agccccatcg acctgagcgt ggaaacgcgt
    11161 cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac aaaaatgcca tgaactggca
    11221 ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc tggcagagtg gagcgccccg
    11281 attcaggcac gtcgtcactc tacccgcgta cataatccgg cggtagaaaa gcgtctggcg
    11341 gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg aagtgcgtgc cgaagcccag
    11401 cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga ttggttcttt cccgcaaacc
    11461 acggaaattc gtaccctgcg tctggatttc aaaaagggca atctcgacgc caataactac
    11521 cgcacgggca ttgcggaaca tatcaggcag gccattgttg aacaggaacg tttgggactg
    11581 gatgtgctgg tacatggcga ggccgagcgt aatgacatgg tggaatactt tggcgagcac
    11641 ctcgacgggt ttgtctttac gcaaaacggt tgggtacaga gctacggttc ccgctgcgtg
    11701 aagccaccga ttgttattgg tgacgttagt cgcccggcac cgattaccgt ggagtgggcg
    11761 aagtatgcgc aatcgctgac cgacaaaccg gtgaaaggga tgctgacggg gccggtgacc
    11821 atactctgct ggtcgttccc gcgtgaagat gtcagccgtg aaaccatcgc caaacagatt
    11881 gcgctggcgc tgcgtgatga ggtggccgat ctggaagcgg cagggattgg tatcatccag
    11941 attgacgaac cggcgctgcg cgaaggttta ccactgcgtc gtagcgactg ggacgcctat
    12001 ctccagtggg gcgtagaggc cttccgtatc aacgccgccg tggcgaaaga tgacacacaa
    12061 atccacactc acatgtgtta ttgcgagttc aacgacatca tggattccat tgcggcgctg
    12121 gatgcagacg tcatcaccat cgaaacctcg cgttccgaca tggagttgct ggagtcgttt
    12181 gaagagtttg attatccgaa tgaaatcggt ccaggcgtct atgacattca ctcgccaaac
    12241 gtaccgagcg tggaatggat tgaagccctg ctgaagaaag cggcaaaacg cattccggca
    12301 gagcgcctgt gggtcaaccc agactgtggc ctgaaaacgc gcggctggcc agaaacccgc
    12361 gcggcactgg cgaacatggt gcaggcggcg cagaatttgc gtctggggta aatccaaacc
    12421 gggtggtaat accacccggt cttttctcat tacagcgact tcttcccacc atactgctta
    12481 aaccattcca gcatacgctg ccagccatct ttcgcagatt cggcatgata gctcgggcga
    12541 taatcagcgt tgaatgcatg cccggcatcc gggtagacga taatctctgc tttcgcatta
    12601 gcagcccgca gcgcctggcg catcgtttca acgctctcct gcggaatgct gttatcctga
    12661 ccgccatata agccgagaac cggcgcgtta agatcggttg cgatatcaac aggttgtttc
    12721 ggtgaattca gcgacttatc gcccgtcagt ttgccgtacc acgccactgc ggcttttagc
    12781 tgtggattat gcgcggcata cagccaggtg atacgtccac cccagcagaa tccggtgatc
    12841 attaaacgat gaacatcgcc gccgttgcgg gaagcccaac tggcgacatg atcgagatcg
    12901 gccagcacct gcgagtcagg cacttttgct accagaccgc taagcaacgt ggggatatcg
    12961 gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag gtgcgatagc cagatacccc
    13021 tccagcgcca gacggcgaca aatgtcgcgg atatgttcat gcacgccaaa aatttcctgc
    13081 actacaatga ccactggcag tgggccatcg ctttgctttg gtctggcatg gtaagcaggc
    13141 atgttatccc cttgtgaagg gatagaggtg aagcccgcca caatcgcgtc gtccggggtc
    13201 tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa atccagattg ttgtgttgtt
    13261 gccatggtat tctccgtacc cttataaaaa tgttgcgcaa tgttaactat agtcagcatg
    13321 caacaaatca cattgcctga atcggctcat cttttatgca gtcctgcaga atgaagagtg
    13381 atttatgtga tttgaatcac ttttggtggg taaatttatg caacgcattt gcgtcatggt
    13441 gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt gtctttttgc ttcttctgac
    13501 taaaccgatt cacagaggag ttgtatatgt ccaagtctga tgtttttcat ctcggcctca
    13561 ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc tggcgacccg gatcgtgtgg
    13621 aaaagatcgc cgcgctgatg gataagccgg ttaagctggc atctcaccgc gaattcacta
    13681 cctggcgtgc agagctggat ggtaaacctg ttatcgtctg ctctaccggt atcggcggcc
    13741 cgtctacctc tattgctgtt gaagagctgg cacagctggg cattcgcacc ttcctgcgta
    13801 tcggtacaac gggcgctatt cagccgcata ttaatgtggg tgatgtcctg gttaccacgg
    13861 cgtctgtccg tctggatggc gcgagcctgc acttcgcacc gctggaattc ccggctgtcg
    13921 ctgatttcga atgtacgact gcgctggttg aagctgcgaa atccattggc gcgacaactc
    13981 acgttggcgt gacagcttct tctgatacct tctacccagg ccaggaacgt tacgatactt
    14041 actctggtcg cgtagttcgt cactttaaag gttctatgga agagtggcag gcgatgggcg
    14101 taatgaacta tgaaatggaa tctgcaaccc tgctgaccat gtgtgcaagt cagggcctgc
    14161 gtgccggtat ggtagcgggt gttatcgtta accgcaccca gcaagagatc ccgaatgctg
    14221 agacgatgaa acaaactgaa agccaagcgg tgaaaatcgt ggtggaagcg gcgcgtcgtc
    14281 tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc ggccttttgt atttttgcgt
    14341 agcgcctcgc aggaaatgcc tttccaactg gacgtttgta cagcacaatt ctattttgtg
    14401 cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca atcatggttt acgcagttat
    14461 tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt tatcaacatg cgcagcaaaa
    14521 agccgagcaa ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca
    14581 aattacccaa agcgagcact ggcgtgcaga gtgcgagtta ctcaataacg aagtgcgcag
    14641 cctgcaaagt attaacacct ctctggaggc cgatctgcgt gaagtaacca cgcggatgga
    14701 agccgcacag caacatgctg acgataaaat tcgccagatg attaacagcg agcagcgcct
    14761 cagtgagcag tttgaaaacc tcgccaaccg tatttttgag cacagcaatc gccgggttga
    14821 tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg ctacgtgaac aactggacgg
    14881 tttccgccgt caggttcagg acagcttcgg taaagaagca caagaacgcc ataccctgac
    14941 ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg gcccaggaag cgatcaacct
    15001 gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac tggggcgagg tagtattgac
    15061 gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat
    15121 cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc gtgcggctgc cgcagggaaa
    15181 agatgtggtg atcgacgcca aaatgacgct ggtcgcctat gaacgctatt ttaacgccga
    15241 agacgactac acccgcgaaa gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat
    15301 ccgtttgctg ggacgcaaag attatcaaca gctgccgggg ctgcgaactc tggattacgt
    15361 gctgatgttt attcccgttg aacccgcttt tttactggcg cttgaccgcc agccggagct
    15421 gatcaccgaa gcgttgaaaa acaatatcat gctggttagc ccgactacgc tgctggtggc
    15481 gttgcgtact attgccaacc tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat
    15541 cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc
    15601 gattggtcaa agtctcgaca aagcgcagga taactaccgc caggcaatga aaaaactctc
    15661 ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag gagtagaaat
    15721 taaacgcgag attaatccgg atttggctga acaggcggtg agccaggatg aagagtatcg
    15781 acttcggtcg gttccggagc agccgaatga tgaagcttat caacgcgatg atgaatataa
    15841 tcagcagtcg cgctagccca ttaggggtag ttaagccggg tagaaatcta gggcatcgac
    15901 gcccaatctg ttacacttct ggaacaattt tttgatgagc aggcattgag atggtggata
    15961 agtcacaaga aacgacgcac tttggttttc agaccgtcgc gaaggaacaa aaagcggata
    16021 tggtcgccca cgttttccat tccgtggcat caaaatacga tgtcatgaat gatttgatgt
    16081 catttggtat tcatcgtttg tggaagcgat tcacgattga ttgcagcggc gtacgccgtg
    16141 ggcagaccgt gctggatctg gctggtggca ccggcgacct gacagcgaaa ttctcccgcc
    16201 tggtcggaga aactggcaaa gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg
    16261 gacgcgagaa gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg
    16321 ctgaggcgct gccgttcccg gataacacct ttgattgcat caccatttcg tttggtctgc
    16381 gtaacgtcac cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg
    16441 gccgcctgct ggtgcttgag ttctcgaagc caattatcga gccgctgagc aaagcctatg
    16501 atgcatactc cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca
    16561 gctaccgtta tctggcagaa tccatccgta tgcatcccga tcaggatacc ctgaaagcca
    16621 tgatgcagga tgccggattc gaaagtgtcg actactacaa tctgacggcg ggggttgtgg
    16681 cgctgcatcg tggttataag ttctgacagg agaccggaaa tgccttttaa acctttagtg
    16741 acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc gctgaaaaca
    16801 gcccgctcgc gtctgctggg taaagtattg cgcgtggagg taaaaggctt ttcgacgtca
    16861 ttgattctgg tgttcagcga acgccaggtt gatgtactgg gcgaatgggc aggcgatgct
    16921 gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt
    16981 accgcactga ttcgcagtgg tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac
    17041 ttcgttgcgc tggcagatct ggcagagttc gaccctgcgg aactgctggc cccttatacc
    17101 ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg gaggcgcaaa gttcctgcat
    17161 cacggcatta agcgccagca acgttatgtg gcggaagcca ttactgaaga gtggcgtatg
    17221 gcacccggtc cgcttgaagt ggcctggttt gcggaagaga cggctgccgt cgagcgtgct
    17281 gttgatgccc tgaccaaacg gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac
    17341 ggcgcctata tttcatcatt cgcacttttt taagctacgg acttgatgaa ctgatcccca
    17401 aaatgcgtat caccctgccg ctacggctat ggcgatactc attattctgg atgccaaatc
    17461 ggcataaaga caaactttta ggtgagcgac tacgactggc cctgcaagaa ctggggccgg
    17521 tttggatcaa gttcgggcaa atgttatcaa cccgccgcga tctttttcca ccgcatattg
    17581 ccgatcagct ggcgttattg caggacaaag ttgctccgtt tgatggcaag ctggcgaagc
    17641 agcagattga agctgcaatg ggcggcttgc cggtagaagc gtggtttgac gattttgaaa
    17701 tcaagccgct ggcttctgct tctatcgccc aggttcatac cgcgcgattg aaatcgaatg
    17761 gtaaagaggt ggtgattaaa gtcatccgcc cggatatttt gccggttatt aaagcggatc
    17821 tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc
    17881 tgcgcccaac cgaagtggtg cgcgagtacg aaaagacatt gattgatgaa ctgaatttgc
    17941 tgcgggaatc tgccaacgcc attcagcttc ggcgcaattt tgaagacagc ccgatgctct
    18001 acatcccgga agtttaccct gactattgta gtgaagggat gatggtgatg gagcgcattt
    18061 acggcattcc ggtgtctgat gttgcggcgc tggagaaaaa cggcaccaac atgaaattgc
    18121 tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt tcgcgacagc tttttccatg
    18181 ccgatatgca ccctggcaac atcttcgtaa gctatgaaca cccggaaaac ccgaaatata
    18241 tcggcattga ttgcgggatt gttggctcgc taaacaaaga agataaacgc tatctggcag
    18301 aaaactttat cgccttcttt aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt
    18361 ctggctgggt gccaccagat accaacgttg aagagttcga atttgccatt cgtacggtct
    18421 gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt tggacatgta ctgttaaatc
    18481 tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc gcaactggtg ttactccaga
    18541 aaaccctgct ctacgtcgaa ggggtaggac gccagcttta tccgcaactc gatttatgga
    18601 aaacggcgaa gcctttcctg gagtcgtgga ttaaagatca ggtcggtatt cctgcgctgg
    18661 tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaac
    18721 tggtttacga cagtttgcgc cagggcaagt atttacagca cagtgttgat aagattgccc
    18781 gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg ttattttctc ggaattggcg
    18841 ctacgttagt attaagtggc acattcttgt tggtcagccg acctgagtgg gggctgatgc
    18901 ccggctggtt aatggcaggt ggtctgatcg cctggtttgt cggttggcgc aaaacacgct
    18961 gattttttca tcgctcaagg cgggccgtgt aacgtataat gcggctttgt ttaatcatca
    19021 tctaccacag aggaacatgt atgggtggta tcagtatttg gcagttattg attattgccg
    19081 tcatcgttgt actgcttttt ggcaccaaaa agctcggctc catcggttcc gatcttggtg
    19141 cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga accaaagcag gataaaacca
    19201 gtcaggatgc tgattttact gcgaaaacta tcgccgataa gcaggcggat acgaatcagg
    19261 aacaggctaa aacagaagac gcgaagcgcc acgataaaga gcaggtgtaa tccgtgtttg
    19321 atatcggttt tagcgaactg ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc
    19381 aacgactgcc tgtggcggta aaaacggtag cgggctggat tcgcgcgttg cgttcactgg
    19441 cgacaacggt gcagaacgaa ctgacccagg agttaaaact ccaggagttt caggacagtc
    19501 tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc cgaactgaaa gcgtcgatgg
    19561 atgaactacg ccaggccgcg gagtcgatga agcgttccta cgttgcaaac gatcctgaaa
    19621 aggcgagcga tgaagcgcac accatccata acccggtggt gaaagataat gaagctgcgc
    19681 atgagggcgt aacgcctgcc gctgcacaaa cgcaggccag ttcgccggaa cagaagccag
    19741 aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac
    19801 cttccccttc gtcgagtgat aaaccgtaaa catgtctgta gaagatactc aaccgcttat
    19861 cacgcatcta attgagctgc gtaagcgtct gctgaactgc attatcgcgg tgatcgtgat
    19921 attcctgtgt ctggtctatt tcgccaatga catctatcac ctggtatccg cgccattgat
    19981 caagcagttg ccgcaaggtt caacgatgat cgccaccgac gtggcctcgc cgttctttac
    20041 gccgatcaag ctgaccttta tggtgtcgct gattctgtca gcgccggtga ttctctatca
    20101 ggtgtgggca tttatcgccc cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct
    20161 gctggtttcc agctctctgc tgttttatat cggcatggcg ttcgcctact ttgtggtctt
    20221 tccgctggca tttggcttcc ttgccaatac cgcgccggaa ggggtacagg tatccaccga
    20281 catcgccagc tatttaagct tcgttatggc gttgtttatg gcgtttggtg tctcctttga
    20341 agtgcctgtg gcaattgtgc tgctgtgctg gatggggatt acctcgccag aagacttacg
    20401 caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc gggatgctgc tgacaccgcc
    20461 ggatgtcttc tcgcaaacgc tgttggcgat cccgatgtac tgtctgtttg aaatcggtgt
    20521 cttcttctca cgcttttacg ttggtaaagg gcgaaatcgg gaagaggaaa acgacgctga
    20581 agcagaaagc gaaaaaactg aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg
    20641 gagtacagga tgtttgatat cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt
    20701 gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg ggctactcat caccggcact
    20761 aacctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca
    20821 acggcgggcg tacatcctca cgacagcagc cagtggcaag ctgcgactga agaagcgatt
    20881 attgagctgg ccgcgcagcc agaagtggtg gcgattggtg aatgtggtct cgactttaac
    20941 cgcaactttt cgacgccgga agagcaggaa cgcgcttttg ttgcccagct acgcattgcc
    21001 gcagatttaa acatgccggt atttatgcac tgtcgcgatg cccacgagcg gtttatgaca
    21061 ttgctggagc cgtggctgga taaactgcct ggtgcggttc ttcattgctt taccggcaca
    21121 cgcgaagaga tgcaggcgtg cgtggcgcgt ggaatttata tcggcattac cggttgggtt
    21181 tgcgatgaac gacgcgggct ggagctgcgg gaattgttgc cgttgattcc ggcggagaaa
    21241 ttgctgatcg aaactgatgc gccgtatctg ctcccgcgcg atctcacgcc aaagccatca
    21301 tcccggcgca acgagccagc ccatctgccc catattttgc aacgtattgc gcactggcgt
    21361 ggagaagatg ccgcatggct ggctgccacc acggatgcca atgtcaaaac actgtttggg
    21421 attgcgtttt agagtttgcg gaactcggtg ttcttcacac tgtgcttaat ctctttatta
    21481 ataagattaa gcaatagcat ggagcgagcc tcaccatcgg gttcggtgaa aatggcctga
    21541 aagccttcga acgcgccttc ggtaataatc accttatcac ccggataagg ggttgccgga
    21601 tcgacaatgt ctttcggttt atataccgat agctgatgaa tcaccgccga tgggactatc
    21661 gctggcgacg cgccaaagcg cacgaagtgg ctgacaccgc gggtcgcgtt gatagtcgtg
    21721 gtatgaatca cttctggatc aaattcgaca aacaggtagt tgggaaacaa tggctcactg
    21781 actgcagtac gttttccacg cacgattttt tccagggtga tcatcggtgc caggcaattc
    21841 acagcctgtc tttcgaggtg ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt
    21901 aaataccagg attgcataat gactcttatc cgtttaatcg gggcgcaagg atagcaaaag
    21961 ctttacgcta agttaattat attccccggt ttgcgttata ccgtcagagt tcacgctaat
    22021 ttaacaaatt tacagcatcg caaagatgaa cgccgtataa tgggcgtaga ttaagaggct
    22081 acaatggacg ccatgaaata taacgattta cgcgacttcc tgacactgct tgaacagcag
    22141 ggtgagctaa aacgtatcac gctcccggtg gatccgcatc tggaaatcac tgaaattgct
    22201 gaccgcactt tgcgtgccgg tgggcctgcg ctgttgttcg aaaaccctaa aggctactca
    22261 atgccggtgc tgtgcaacct gttcggtacg ccaaagcgcg tggcaatggg catggggcag
    22321 gaagatgttt cggcgctacg tgaagttggt aaattattgg cgtttctgaa agagccggag
    22381 ccgccaaaag gtttccgcga cctgtttgat aaactgccgc agtttaagca agtattgaac
    22441 atgccgacaa agcggctgcg tggtgcgccc tgccaacaaa aaatcgtctc tggcgatgac
    22501 gtcgatctca atcgcattcc cattatgacc tgctggccgg aagatgccgc gccgctgatt
    22561 acctgggggc tgacagtgac gcgcggcccg cataaagagc ggcagaatct gggcatttat
    22621 cgccagcagc tgattggtaa aaacaaactg attatgcgct ggctgtcgca tcgcggcggc
    22681 gcgctggatt atcaggagtg gtgtgcggcg catccgggcg aacgtttccc ggtttctgtg
    22741 gcgctgggtg ccgatcccgc cacgattctc ggtgcagtca cccccgttcc ggatacactt
    22801 tcagagtatg cgtttgccgg attgctgcgc ggtaccaaga ccgaagtggt gaagtgtatc
    22861 tccaatgacc ttgaagtgcc cgccagtgcg gagattgtgc tggaagggta tatcgaacaa
    22921 ggtgaaacgg cgccggaagg gccgtatggc gaccacaccg gttactataa cgaagtcgat
    22981 agtttccctg tctttactgt gacgcatatt acccagcgtg aagatgcgat ttaccattcc
    23041 acctataccg ggcgtccgcc tgatgaacct gcggtactgg gcgtggcgtt gaacgaagtg
    23101 tttgtgccga ttctgcaaaa acagttcccg gaaattgtcg atttttacct gccaccggaa
    23161 ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac agtacgccgg acacgcgaag
    23221 cgcgtcatga tgggcgtctg gtcgttctta cgccagttta tgtacactaa atttgtgatc
    23281 gtttgcgatg atgacgtcaa cgcacgcgac tggaacgatg tgatttgggc gattaccacc
    23341 cgtatggacc cggcgcggga tactgttctg gtagaaaata cgcctattga ttatctggat
    23401 tttgcctcgc ctgtctccgg gctgggttca aaaatggggc tggatgccac gaataaatgg
    23461 ccgggggaaa cccagcgtga atggggacgt cccatcaaaa aagatccaga tgttgtcgcg
    23521 catattgacg ccatctggga tgaactggct atttttaaca acggtaaaag cgcctgatgc
    23581 gcgtttgttt tgccctattt atcgatccga cagagaaagc gcatgacaac cttaagctgt
    23641 aaagtgacct cggtagaagc tatcacggat accgtatatc gtgtccgcat cgtgccagat
    23701 gcggcctttt cttttcgcgc tggtcagtat ttgatggtag tgatggatga gcgcgacaaa
    23761 cgtccgttct caatggcttc gacgccggat gaaaaagggt ttatcgagct gcatattggc
    23821 gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa
    23881 atcgtggtcg atattcccca cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg
    23941 attttgattg cgggcggcac cgggttctct tatgcccgct cgattttgct gacagcgttg
    24001 gcgcgtaacc caaaccgtga tatcaccatt tactggggcg ggcgtgaaga gcagcatctg
    24061 tatgatctct gcgagcttga ggcgctttcg ttgaagcatc ctggtctgca agtggtgccg
    24121 gtggttgaac aaccggaagc gggctggcgt gggcgtactg gcaccgtgtt aacggcggta
    24181 ttgcaggatc acggtacgct ggcagagcat gatatctata ttgccggacg ttttgagatg
    24241 gcgaaaattg cccgcgatct gttttgcagt gagcgtaatg cgcgggaaga tcgcctgttt
    24301 ggcgatgcgt ttgcatttat ctgagatata aaaaaacccg cccctgacag gcgggaagaa
    24361 cggcaactaa aactttattc agtggcattt agatctatgt cgcatccggc attcggcaca
    24421 cgatgcctga tgcgacgctg gcgcgtctta tcaggcctac attggtgccg gatcggtaga
    24481 ccggataagg cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgtcgca
    24541 ataccctgac ccagaccgat acacatcgtc gccagaccaa actggacgtc tttgcgctcc
    24601 atcagattta gcagcgtggt gctgatacgc gcaccggaac agcccagcgg atgacccagc
    24661 gcgatcgcgc caccgttgag gttgatcttc tcgtcaatct gctccattag ccccagatct
    24721 ttaatacatg gcaggatctg cgcggcaaag gcttcattca tttcaaacac gccgatatca
    24781 ctgacagaaa gccccgcttt tttcagcgcc agcttcgagg ccggaaccgg gccgtaaccc
    24841 ataatcgatg ggtcacaacc aacgaccgcc atcgaacgca cgcgggcgcg cggcttaaga
    24901 cctaattcat gggcgcggct ttcactcatc accagcatgg cagctgcgcc atcggaaagc
    24961 gcagaagatg tacccgccgt taccgtaccg cttactggat caaacgccgg acgcagcgtg
    25021 gcgagggctt ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcagg
    25081 acgccgtcgg catcgtgacc accggtcggg ataatttcat ttttaaatgc acccgactgc
    25141 gtggcggccc aggcgcgggc gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg
    25201 ataccgtgca tacgcgccag catttctgcc gttaagccca tcatgcccgc cgctttggcg
    25261 acattgcggc tcaggccggg gtgaaaatcg acgccgtggc tcattggcac atggcccata
    25321 tgctccacgc cgccgacaag acatgcctgc gcatcgccag tcatgatcat tcgtgctgcg
    25381 tcatgcaatg cctgcatgga tgaaccacac aagcgattga cggtcaccgc cgggacagag
    25441 tgtggtactt ctgccagcag cgccgcgtta cgggcgatat taaaaccctg ctccagcgtc
    25501 tgctgcacac aaccccagta aatatcgtcg agggccgccg cttccagcgc cgggttacgc
    25561 gccagcaggc tacgcattaa atgagcggag agatcttctg cacgcacgtt acgaaaagca
    25621 ccgcccttcg aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt
    25681 gtgactcctt aagccgtttt caggtcgcca accggacggg ctggctcaac cggaggatag
    25741 tacggttcgt tatgacgcgc tttattacgc agaccttccg gcacttcata cagcgggccg
    25801 aggtgctgat attgctgtgc catatcgagg tattttgcgc taccgagggt gtccagccag
    25861 cggaacgcgc cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct
    25921 tccgccggag tggcgataat gccttcctcc agacagcgca ccacttcgtt gaccatcggg
    25981 atcatcatgc gggcgataat ctcttcttcg ctgaaatcgc gcttcggctg gctcacttct
    26041 gccagcaggt cttcaacggc ggcgtcttct tctttcttcg gcttaccttt gctgtcttct
    26101 ttataacgcc agaaaccgag gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg
    26161 tcgatggcat cgcggtaatc tttctgcatc cgctgcggga agcctgctgc catgacagcc
    26221 tgagcgtgat gcgcggtatc aatgcccacg acatccagca gatatgccgg acccataggc
    26281 cagccaaact gtttttccat cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc
    26341 agctggctga aaccggcgaa atacgggaac agcacgcggt taacaaagaa gccggggcag
    26401 tcgttaacca caatcggcgt cttgcccatc ttgctcgccc aggcgacaac tttcgcgatg
    26461 gtttcgtcgg agcttttctc gccgcgaata atttctacca acggcatccg gtgtaccggg
    26521 ttaaaaaagt gcattccgca gaagttttcc gggcgttcca gcgcgttggc cagttcgctg
    26581 ataggaatgg ttgaagtgtt agacgccagc acggtatccg ggcgtacttt ttgttcggtt
    26641 tctgccagta cggctttttt cactttcggg ttttcgacaa ccgcttctac cacaacatcc
    26701 acgcggtcaa acccggcgta gtcgagcgtt gggtggattg tggagatcac gccagccagt
    26761 ttcagaccat cgatcttgcc gcgctcaagc tgcttgttca gcagtttcgc ggcttcggtc
    26821 atgccgaggg ttaacgactt gtcgttgata tctttcatga caaccggcac gcctttccac
    26881 gcagactggt aagcgatgcc gccgcccata atgcctgcac ccagcaccgc ggcctgtttc
    26941 ggggtttcaa cgtctttggt gagtttcttc gctttgcctt ttacatattg atcgttaagg
    27001 aaaatgccga ccagtgcgcg ggcttcgttg gtatgcgcca gcgggacaaa acttttgttt
    27061 tccaggttta aggcttcttc acgaccaaaa cgggccgcag cttcaatggt ttttactgcg
    27121 gtgatggggg ccggataatg tttccccgct gtttgtgcga ccatcccttt agcgatggtg
    27181 aagctcatgg tggcttcaat cttgctcagt tttagtggtt ccagcttcgg ctgacgtttt
    27241 gctttccagt cgaggtcgcc gttaatggcc tggcgtaaaa ccgcctttgc gccttcaacc
    27301 agtttttctg ctttgactac gccatccacc agaccgattt tcagcgcctg atccgcgccg
    27361 acatctttac cggcggcaat gatttccagc gcactgtcag cgcccagcat acgtggcata
    27421 cgtacagaac cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc
    27481 agatccggcg tcgccagacg ataatcggtc gccagcacgc attcgcagcc accgcccagc
    27541 gcatagccgt tggcggcagc aatggtcggc accggcagat cttccaggcg attaaacacg
    27601 ctattggcaa agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa
    27661 aattcggtga tatcagcacc gacgataaag gctgctttgt tcgaacgcag cagcagccct
    27721 tttagatctg attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca
    27781 gtgtcgagtt tattaactga acctggggca tcaaatacca gttcggcaat gccatcttcc
    27841 agccagtcaa ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag
    27901 gtgatctggt cgtaccagat gagtcgaagt gtgtattttg tgttaaaaat atgcaaataa
    27961 aagattaaag aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat
    28021 gtgctaagat gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg
    28081 cctcactcta taaaaatcat atagctacct tacaggaacg gactcgcgat gcgctggcgc
    28141 gcttcaagct ggatgcgtta cttattcact ccggcgaact gttcaacgtt tttctcgacg
    28201 atcatcccta tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg
    28261 tgccaaactg ctggctgctg gtggatggcg tgaacaagcc gaaactgtgg ttctatctgc
    28321 cggttgatta ctggcacaac gtcgaaccgc tgccgacctc tttctggact gaagacgtgg
    28381 aagtgatcgc gctgccgaaa gccgatggca ttggtagcct gctgcctgct gcgcgcggga
    28441 atatcggtta tatcggtccg gtgccggaac gtgcgctgca actgggtatt gaggccagca
    28501 atatcaaccc gaaaggggtg atcgactacc tgcattacta ccgctccttc aaaaccgagt
    28561 acgaactggc ctgtatgcgt gaagcgcaga aaatggcggt caacggtcat cgcgcggcag
    28621 aagaagcgtt ccgttctggc atgagcgagt tcgatatcaa cattgcctat ctgaccgcga
    28681 ccggtcatcg tgataccgac gtaccttaca gcaacattgt ggcgctcaac gaacacgctg
    28741 cggtgttgca ttacaccaaa ctggaccacc aggcaccgga agagatgcgc agcttcctgc
    28801 tggatgccgg ggccgaatat aacggctatg cggctgacct gacccgtacc tggtcggcaa
    28861 aaagtgacaa cgactacgca cagctggtga aagacgtaaa tgatgaacaa ctggcgctga
    28921 tcgctaccat gaaagcaggc gtcagctatg tggattacca catccagttc catcagcgca
    28981 tcgccaaact gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa gcgatggtcg
    29041 aaaacgatct caccggaccg tttatgccgc acggtattgg ccatccgctg ggcctgcagg
    29101 tgcatgacgt agccggtttt atgcaggatg atagcggtac gcacctcgcg gcaccggcaa
    29161 aatatccgta cctgcgctgc acccgtattc tccagccggg catggtgtta accatcgaac
    29221 cgggtatcta cttcattgaa tcgctgctgg caccgtggcg tgaagggcag ttcagcaagc
    29281 acttcaactg gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca
    29341 acgtggtgat ccacgaaaac aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat
    29401 ggaaagctgg ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaaaagccg
    29461 cttcataacg atgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc
    29521 ggtgcgggca gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc
    29581 ggatgattct caacagctgg gtttctctga cgacggggag ccggcgggaa cggcaggtaa
    29641 accgatgctc gcccagctaa tgggcagcgg cgtcggggaa attaccgctg tggtagtgcg
    29701 ctactacggc ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt
    29761 gaatcaggcg ctgcgccagc taacgaccca acgcaagacg ccattaaccg aatatacttt
    29821 gcaatgtgaa tatagtcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa
    29881 aattatcaac agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa
    29941 agtggctgaa ttttccgcaa agctggcgga ttttagccgt ggttcattgc aattgttagc
    30001 gattgaagaa taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt
    30061 cgcgccatta cccgaatcgt tggactactg gtcatcttat tttcagggac catgattatc
    30121 cctgggctgg tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt
    30181 tttgtcgccc tcgccattgg ctctatgctg tggtggccga accgcaaaga gaagggcgaa
    30241 ctgaaatccc gtgaggggtt tctgatagtg gtgctgtttt ggaccgtgct gggtagcgtc
    30301 ggtgcgctcc cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt
    30361 gaatctttct ctggcctgac caccaccggg gccactacgc tggtggggct ggattcgctc
    30421 cctcatgcca tcctctttta tcgccagatg ctgcaatggt ttggcgggat ggggatcatc
    30481 gtgttagcgg ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca
    30541 gaaatgcccg gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa
    30601 accctgtggt tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc
    30661 ggaatggatg cctttgatgc catcggccat agctttgcga ctatcgctat tggcggcttc
    30721 tcgacacatg atgccagtat cggttatttc gacagcccga ctattaacac tatcattgct
    30781 atcttcctgc tgatctccgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt
    30841 agtctgaagg tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagtttacg
    30901 ctggtggtta tttgtacact cgtactgtgg tttcataatg tctacagttc ggcgctgatg
    30961 acaattaacc aggcgttttt ccaggtggta tcgatggcga caaccgccgg gtttacgact
    31021 gacagcattg cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc
    31081 ggcggttgcg ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gctgctgttt
    31141 aagcagggga accgtgagct gaaacgactg gtgcatccga acgccgtcta tagcattaag
    31201 ctggggaatc gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc
    31261 tatgcattgg tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgacttt
    31321 tctgcctttg cctcagttgt tgcgacattg aataacctgg ggccagggct tggcgtggtt
    31381 gctgataact ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg
    31441 tttggtcgtc tcgaggtctt tacattgctg gtgctcttta ccccgacttt ctggcgtgaa
    31501 tgatggagta atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg
    31561 agattgcctc ctacctggct tcggaactga aagaactggg gatccagacg gatgtcgcca
    31621 atgtgcaccg cattgaagaa ccacaatggg aaaattatga ccgtgtggtc attggtgctt
    31681 ctattcgcta tggtcactac cactctgcgt tccaggaatt tgtcaaaaaa catgcgacgc
    31741 gactgaattc gatgccgagc gccttttatt ccgtaaacct ggtggcgcgc aaaccggaga
    31801 agcgtactcc acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc
    31861 ccgatcactg cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgatc
    31921 gttttatgat caagctaatt atgaagatgt caggcggtga aacggatacg cgcaaagaag
    31981 ttgtctatac cgattgggag caggtggcga atttcgcccg agaaatcgcc catttaaccg
    32041 acaaaccgac gctgaaataa gcataaagaa taaaaaatgc gcggtcagaa aattatttta
    32101 aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga cacggaacaa
    32161 cggcaaacac gccgccgggt cagcggggtt ctcctgagaa tctcaacaga gaaaagtaaa
    32221 aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac cccgcgccgc tgagaaaaag
    32281 cgaagcggca ctgctcttta acaatttatc agacaatctg tgtgggcact cgaagatacg
    32341 gattcttaac gtcgcaagac gaaaaatgaa taccaagtct caagagtgaa cacgtaattc
    32401 attacgaagt ttaattcttt gagcatcaaa cttttaaatt gaagagtttg atcatggctc
    32461 agattgaacg ctggcggcag gcctaacaca tgcaagtcga acggtaacag gaaacagctt
    32521 gctg
//
LOCUS       DAZFYC010000046        32504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFYC010000046 DAZFYC010000000
VERSION     DAZFYC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.046"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..189)
                     /locus_tag="ACHKW5_002398"
     CDS             complement(<1..189)
                     /locus_tag="ACHKW5_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7511782.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFT"
     gene            complement(232..1029)
                     /gene="rfaP"
                     /locus_tag="ACHKW5_002399"
     CDS             complement(232..1029)
                     /gene="rfaP"
                     /locus_tag="ACHKW5_002399"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO7511783.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(1022..2146)
                     /gene="waaG"
                     /locus_tag="ACHKW5_002400"
     CDS             complement(1022..2146)
                     /gene="waaG"
                     /locus_tag="ACHKW5_002400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7511784.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2143..3201)
                     /gene="rfaQ"
                     /locus_tag="ACHKW5_002401"
     CDS             complement(2143..3201)
                     /gene="rfaQ"
                     /locus_tag="ACHKW5_002401"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO7511785.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            3614..4891
                     /gene="waaA"
                     /locus_tag="ACHKW5_002402"
     CDS             3614..4891
                     /gene="waaA"
                     /locus_tag="ACHKW5_002402"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO7511786.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4899..5378
                     /gene="coaD"
                     /locus_tag="ACHKW5_002403"
     CDS             4899..5378
                     /gene="coaD"
                     /locus_tag="ACHKW5_002403"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO7511787.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5417..6226)
                     /gene="mutM"
                     /locus_tag="ACHKW5_002404"
     CDS             complement(5417..6226)
                     /gene="mutM"
                     /locus_tag="ACHKW5_002404"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO7511788.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWVIIHLGMSGSLRILPEELPPEKHDHVELVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6324..6491)
                     /gene="rpmG"
                     /locus_tag="ACHKW5_002405"
     CDS             complement(6324..6491)
                     /gene="rpmG"
                     /locus_tag="ACHKW5_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO7511789.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(6512..6748)
                     /gene="rpmB"
                     /locus_tag="ACHKW5_002406"
     CDS             complement(6512..6748)
                     /gene="rpmB"
                     /locus_tag="ACHKW5_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO7511790.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(6965..7633)
                     /gene="radC"
                     /locus_tag="ACHKW5_002407"
     CDS             complement(6965..7633)
                     /gene="radC"
                     /locus_tag="ACHKW5_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7511791.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7805..9025
                     /gene="coaBC"
                     /locus_tag="ACHKW5_002408"
     CDS             7805..9025
                     /gene="coaBC"
                     /locus_tag="ACHKW5_002408"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO7511792.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9003..9461
                     /gene="dut"
                     /locus_tag="ACHKW5_002409"
     CDS             9003..9461
                     /gene="dut"
                     /locus_tag="ACHKW5_002409"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO7511793.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRVCLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            9568..10164
                     /gene="slmA"
                     /locus_tag="ACHKW5_002410"
     CDS             9568..10164
                     /gene="slmA"
                     /locus_tag="ACHKW5_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO7511794.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10201..10842)
                     /gene="pyrE"
                     /locus_tag="ACHKW5_002411"
     CDS             complement(10201..10842)
                     /gene="pyrE"
                     /locus_tag="ACHKW5_002411"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO7511795.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(10908..11624)
                     /gene="rph"
                     /locus_tag="ACHKW5_002412"
     CDS             complement(10908..11624)
                     /gene="rph"
                     /locus_tag="ACHKW5_002412"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO7511796.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            11751..12614
                     /gene="yicC"
                     /locus_tag="ACHKW5_002413"
     CDS             11751..12614
                     /gene="yicC"
                     /locus_tag="ACHKW5_002413"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO7511797.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12835..13659
                     /gene="dinD"
                     /locus_tag="ACHKW5_002414"
                     /pseudo
     CDS             12835..13659
                     /gene="dinD"
                     /locus_tag="ACHKW5_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            13951..14568
                     /gene="yicG"
                     /locus_tag="ACHKW5_002415"
     CDS             13951..14568
                     /gene="yicG"
                     /locus_tag="ACHKW5_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO7511798.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(14565..16247)
                     /gene="ligB"
                     /locus_tag="ACHKW5_002416"
     CDS             complement(14565..16247)
                     /gene="ligB"
                     /locus_tag="ACHKW5_002416"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO7511799.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVTEWKENAQIKKLGSWLAAQQITGFEP"
     gene            16505..17128
                     /gene="gmk"
                     /locus_tag="ACHKW5_002417"
     CDS             16505..17128
                     /gene="gmk"
                     /locus_tag="ACHKW5_002417"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO7511800.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17183..17458
                     /gene="rpoZ"
                     /locus_tag="ACHKW5_002418"
     CDS             17183..17458
                     /gene="rpoZ"
                     /locus_tag="ACHKW5_002418"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO7511801.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            17477..19585
                     /gene="spoT"
                     /locus_tag="ACHKW5_002419"
     CDS             17477..19585
                     /gene="spoT"
                     /locus_tag="ACHKW5_002419"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO7511802.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            19592..20281
                     /gene="trmH"
                     /locus_tag="ACHKW5_002420"
     CDS             19592..20281
                     /gene="trmH"
                     /locus_tag="ACHKW5_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO7511803.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            20287..22368
                     /gene="recG"
                     /locus_tag="ACHKW5_002421"
     CDS             20287..22368
                     /gene="recG"
                     /locus_tag="ACHKW5_002421"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO7511804.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(22353..23219)
                     /locus_tag="ACHKW5_002422"
     CDS             complement(22353..23219)
                     /locus_tag="ACHKW5_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511805.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(23222..24427)
                     /gene="gltS"
                     /locus_tag="ACHKW5_002423"
     CDS             complement(23222..24427)
                     /gene="gltS"
                     /locus_tag="ACHKW5_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO7511806.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            24707..26098
                     /gene="xanP"
                     /locus_tag="ACHKW5_002424"
     CDS             24707..26098
                     /gene="xanP"
                     /locus_tag="ACHKW5_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO7511807.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            26219..27928
                     /gene="yicH"
                     /locus_tag="ACHKW5_002425"
     CDS             26219..27928
                     /gene="yicH"
                     /locus_tag="ACHKW5_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7511808.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(27981..30299)
                     /gene="yicI"
                     /locus_tag="ACHKW5_002426"
     CDS             complement(27981..30299)
                     /gene="yicI"
                     /locus_tag="ACHKW5_002426"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO7511809.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(30309..31691)
                     /gene="yicJ"
                     /locus_tag="ACHKW5_002427"
     CDS             complement(30309..31691)
                     /gene="yicJ"
                     /locus_tag="ACHKW5_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO7511810.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            31984..32078
                     /locus_tag="ACHKW5_002428"
     tRNA            31984..32078
                     /locus_tag="ACHKW5_002428"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32018..32020,aa:Sec,seq:tca)
     gene            32157..32318
                     /gene="rhuM"
                     /locus_tag="ACHKW5_002429"
                     /pseudo
     CDS             32157..32318
                     /gene="rhuM"
                     /locus_tag="ACHKW5_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         8066 a         8699 c         8033 g         7706 t
ORIGIN      
        1 tgtgaaaaca tgaaattcac aagagatttc tctattgttt aatagaatag atgcgatggc
       61 tacaccacat ccaaaaagaa aatttctgtc aataccaaat gctatatcaa gagtattttt
      121 agatcttata ttttgataat gaaaattata ctcaaaaagg atgatgtcac tatcattgag
      181 ttgagacata tttattctgt atccaaaatt ttggcaaaaa taagttacgg gttacaacga
      241 ttttcgaatc gttctttccc tgatttttgt ggcttttgct tctgcttgcg acagcagtcc
      301 ttgttcctgc ttgagaatgt ctttaagcgg ggcggcaaaa tacactttca taaaccgcca
      361 gatatcccgc tgagtcaggc cgatattcat cgaagaaaaa taaagcccaa taagatcttt
      421 atcccgccaa cgacgtggaa cgcgcgtgcg aagctgcgcc cggtgcaggt caattaccga
      481 aatttttaac tcctcttcct taccggaaaa aggcaagtgc agcaggaaat gacagatata
      541 acagtcacgg tggttaatgc ccgcagcatg catatcgcgc accatcgtcg cgacacgctt
      601 aataagcata cgctttacgc gaacatctgg agggttagtc gcccagtcag cactgtaatc
      661 ttcaagactt atggttggtg tcagatcttc ggtaataata aatgaagttc tggtcagcgg
      721 attcatgcct ttttcgccaa atgccacccc atacatagta tcaacgccga catcccgcag
      781 tcgatgaatc gcattccatt cgcggtcagc gcctaatact ggcatccgca atgagagtaa
      841 atttttgatt atctctttca gggtcgtgcc gcgatgccat ttgagaaaat agcttttgcc
      901 cgccatttca aagcgcagag tacggcgagt ttccagttca cgaaatacct caccctgcaa
      961 ggttttaact tcctcaaaag gatctttgcc gcgccataac gtggcaaacg gctctttaag
     1021 ttcaaccatc cagaccaccc gttatgatat ccgccgcttt ctctggcaga ctgtataaat
     1081 cttgtgtatc agcataatgt cgcgcatttt ccgcccaggc ctggcgcaat gaagattgcg
     1141 ttaacgcttt gcgtaaaatc tcattcaatg tttcctggcg gaatggctca gcaatagcct
     1201 cgccgcaatt agcgtcgaca atataatgcg catagccaca aacggctgtt gttagtaccg
     1261 gtaatcctgc agtaatcgct tccagcagca caattcccgc cgcttcctgg taggcaggat
     1321 gcagtaataa atccgccgcc gccattaatt ccgagacatc attgcgcccc gagaaaaagt
     1381 gaacattact gcgcacgccg cgtttttctg ccagcgcctc aaattttcgc ggtttatcct
     1441 gcccaacaac atatagcaat gtgttgtggc gcagcgaatc cggtaacgaa gcaagtgctt
     1501 caatggaacg atcgacacct ttacgcgtga agtctgaacc gacctgcaac aataaatatt
     1561 gttgttcggt tattccattc ttcttacgga agatttcacg gctattggct ggctgctggc
     1621 tatatttacg atcaggataa atccccggtg gcagaatatg aaaacgctcc gcttcagtct
     1681 gataatgttt ctggaaatcg gcgatttgct tatctgtcag catcagcaac tgtgtcggct
     1741 taccctgttc gaaggttgcc cgctcaaagg cggcataatg gcgataacgt gacgtcaggc
     1801 gatagaaaaa gcctttttcc tgcgctactt tctcggcata acaaacatca gcggcataat
     1861 aaacgtccag ccccggcatt ttgttgaatc caacgacttt atcgacggga tgttcgcgta
     1921 aatgtttttg cacccaggca aaatactccg cattgcgccc gtgattggta tgcgatttaa
     1981 ccggcacttt gatcagttca aatacatcag ggcattcgcc ttcccacgac tgggtataaa
     2041 cccgaacatg atgacctcgg gcggcgactg tctgagcaat acgcataaaa tcacgctgca
     2101 aaccgccaaa gggaaaatat ttatataaac aaaaagcaac gatcataacg acgtacctgt
     2161 cgtggaggag ggcagtaatt tatcgacagc agcaatgaca tctgccgccg gaataaccga
     2221 aagatacatc tcatttcggt cacgctgatc gcgcgttggc atttcccggt aatctcccgc
     2281 ccagaattga atcatgttat ttgaccaggg acgccagaaa atatgatctg tcgcaccaaa
     2341 cagcgatatc agcggcgtat taactgcagc ggcaatatgc gccggtgcgg aatcaacgcc
     2401 aataaacagc tgcgcatgat cgattaacgc accaagttcc gggaaggtca cctttccagc
     2461 cagcgccgtt actggtggcg tctggcatcc ctgcgcaatt tcattgacgc aggccagatc
     2521 gtctttatct gggccggacg tcagaacgac ttcataacca cgagcatgta aggcatcaat
     2581 cacagcggaa aacttggcgt tgtcccagca tttgaagatt tgccgcgccg taggttggat
     2641 aaccacataa ttttgtccaa caccagcgtg atcaagttcg cgacgcatac gtttccagct
     2701 tgcaggcggg taactcatgg ttgtctgctt caccaacgaa tcaagtccca atggtgtcag
     2761 cacggataag ttactttcca ccacatttcc accctgcaac ggcaccaaat gggtgaaact
     2821 tttacgccaa aaagcagact gccgatgatg ataatcctgg gaaattttca cacgagcatt
     2881 taataagcga accagtatag caaccatcca ttgatcggta agattgacga taaggtcata
     2941 cttattggca cgtaatacct tgatgagatg aaaaaagttg gcaatttttt ctgaggcttt
     3001 tgctttttta ttttttatgc cgtagagcgc gttaatctct ggattttcag acaggatcgg
     3061 gatggtgtcc tgataaagca gcacatcgat ttttgcgtca gggtaatttt ttttcagcga
     3121 actaatgacg ggagtagtta ataacatatc cccatgaaaa cgcatcttaa tgagcaaaat
     3181 tcttcgaaat ggcttatcca caagtaactc tttttgttga gattatccga ttaaggtgaa
     3241 gcagaaaaag cacgctaccg ccccaggctc aacagctacc tgaatactga taccgcactg
     3301 atattgacgc gctagtgtat cacttctttc gcggcaatcc gacccgaatg atttttaagc
     3361 acattgttga ttttcaataa agaaatcaat caaataaaat cattagcaac catattcttt
     3421 cactgttgct ctgcgattcc ttcctgcacg tacagcagaa atcaatgaat cgcataaaaa
     3481 tacaaaaaaa gtatagatgt tcagcaagaa ttggcgggta aattgctatt taagtcaaaa
     3541 acgggaaaag taatggtaaa gccacagcta aatacataga atccccagca catccataag
     3601 tcagctattt actatgctcg aattgcttta caccgccctt ctctacctta ttcagccgct
     3661 gatctggata cggctctggg tgcgcggacg taaggctccg gcctatcgaa aacgctgggg
     3721 tgaacgttac ggtttttacc gccatccgct aaaaccaggc ggcattatgc tgcactccgt
     3781 ctccgtcggt gaaactctgg cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc
     3841 tgatttaccg attaccgtta caaccatgac gccaaccggt tcggagcgcg tacaatcggc
     3901 tttcgggaag gatgttcagc acgtttatct gccgtatgac ctgcccgatg cactcaaccg
     3961 tttcctgaat aaagtcgacc ctaaactggt gttgattatg gaaaccgaac tatggcctaa
     4021 cctgattgcg gcgctacata aacgtaaaat tccgctggtg atcgctaacg cgcgactctc
     4081 tgcccgctcg gccgcaggtt atgccaaact gggtaaattc gtccgccgct tgctgcgtcg
     4141 tattacgctg attgctgcgc aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc
     4201 aaaaaataac caggtaaccg ttaccggtag cctgaaattc gatatttctg taacgccgca
     4261 gttggctgct aaagccgtga cgctacgccg ccaatgggca ccacaccgcc cggtatggat
     4321 tgccaccagc actcacgaag gtgaagagag cgtggtcatt gccgcacatc aggcattgtt
     4381 acagcaattc ccgaatttat tgctcatcct ggtaccccgt catccagaac gcttcccgga
     4441 tgcgattaac cttgtccgcc aggctggact aagctatatc acacgctctt caggggaagt
     4501 cccctccacc agcacgcagg ttgtagttgg cgatacgatg ggcgagttga tgttactgta
     4561 cggcattgcc gatctcgcct ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc
     4621 gctggaagct gccgcacacg ctattccggt attgatgggg ccgcatactt ttaactttaa
     4681 agacatttgc gcgcggctgg agcaggcaag cgggctgatt accgttaccg atgccactac
     4741 gcttgcaaaa gaggtgtcct ctttactcac cgacgccgat taccgtagtt tctatggccg
     4801 tcatgccgtt gaagtactgt atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct
     4861 ggaaccttac ctgccaccga aaacgcattg aggttgttat gcaaaaacgg gcgatttatc
     4921 cgggtacttt cgatcccatt accaatggtc atatcgatat cgtgacgcgc gccacgcaaa
     4981 tgttcgatca cgttattctg gcgattgccg ccagccccag taaaaaaccg atgtttaccc
     5041 tggaagagcg tgtggcgctg gcacagcagg caaccgcgca tctggggaac gtggaagtgg
     5101 tcgggtttag tgatttgatg gcgaacttcg cccgtaatca acacgctacg gtgctgattc
     5161 gtggcctgcg tgcggtggcg gattttgaat atgaaatgca gctggcgcat atgaatcgcc
     5221 acttaatgcc ggaactggaa agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct
     5281 cttcatcgtt ggtgaaagag gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg
     5341 agaatgtcca tcaggcgctg atggcgaagt tagcgtagcg tttatgccgg atggtatgcc
     5401 atccggcgcg catgaattac ttctggcact gccgacaata aaacgttgcc cgctgcgcat
     5461 gtttagtcgc cacaatcggc gtaccgcaca cccgacacgg ctcgcctttt cgcccgtaaa
     5521 cctgcaattc ctgagcgaaa tagcccggtt taccatcact ttgcagaaaa tctttcagcg
     5581 ttgtaccacc ctgctcaatc gaacgcagca acaccgcttt aatcacccga gctaacaatt
     5641 cacactctgc cagcgacagt gatgacgcca gccgatccgg atggatcccc gccgcaaaca
     5701 gtgattcgct ggcatagata ttccctaccc ctaccaccag cttgttatcc atcagccacg
     5761 gtttaatcgc cgtttttttc ttcgcgcact tctgatgcag atactcacca ttgaaatcgt
     5821 cgctaagcgg ctccggtcca agatgggtca gcacattatg cccttccagc tctttggtcc
     5881 acagccaggc accaaagcgg cgcggatcgg tgtagcgcag cactttgccg ttgctcatca
     5941 ccaattccac atggtcatgc ttttcagggg gaagttcttc tggaaggatg cgcaggctgc
     6001 cagacatccc taaatgaatg ataacccagc cctcaggcag ctccagcagc agatatttag
     6061 cccgccgctg cacgctaagc actggttggt cgcttaaacg gtagatctct tctgaaaccg
     6121 gccagcgcaa gcgtccgttg cgcaccactg catgaagaat ggttgcacca acgagatgcg
     6181 gttctatgcc gcggcggctg gtttcaactt cgggtaattc aggcatagca tctccaggaa
     6241 tgaacagatg cagtcaatat gggggcaagc agataacaaa aaaccccgcc ggagcgaggt
     6301 tttttgttac atcaaagcga gaattatttg attttcgctt ctttgtagat cacgtgctgg
     6361 cgaacaactg gatcgaattt tttcagttcc agtttttccg gcttagtacg tttgttcttc
     6421 gtagtggtat agaagtgacc agtaccagca gaagaaacca gcttgatttt ctcacgaata
     6481 cctttagcca tgatttattt cctctaagta cttagtactt ttcgccacgg gcacgcagtt
     6541 cagccagaac tgtatcgatg ccttttttat cgattacacg cataccttta gcagatacgc
     6601 gcagggtgac aaaacgcttc tcgctctcaa cccagaaacg gtgagagtgc aggttcggca
     6661 ggaaacggcg tttagtcgcg ttcagtgcgt gggaacggtt gttaccggtc accggacgct
     6721 tgccagtaac ttggcagact cgggacatgt ctattctcca aaaatcaaat tagctcgagc
     6781 ttcgtatggg gtattggcgc ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg
     6841 aactctcgat tgccaggccc aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac
     6901 gctgactcag cgatgtgctc aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag
     6961 cgggttaaat ccagccgcgt tcggcaaaag aaacatactc tccacgccca atcacgatat
     7021 ggtcgagcac gcgtaaatcc atgaactgac aactctttat tatccgttca gtaatgagtt
     7081 tatccgcttt actgggttca gcacaacccg aagggtgatt atgtgcaagg atcagcgccg
     7141 aggcgtttat ttttatcgct tcgcggataa tttcccgagg atggacttca acatggttta
     7201 gcgtgccgga aaaaagacgg ctatgcgtta taacccggtg ttgggagtcg agaaagatca
     7261 ccataaagat ctcccgctcc tcacccgtga gctggctttg taaaaattcc ctcgtcatct
     7321 ccgggctgag taaagggctt tcttcacgca tccgcacgtt gtagtaacgc cgcgccagtt
     7381 cagcaatccc ctttaactgg gcaaatttcg ccacgccaat tccatgaacg ccactaaatt
     7441 gttcatattc agaggttaac aagccataaa gagagccgaa attctccagc atctcttttg
     7501 ccagggttaa tacatcttta ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga
     7561 catccgttaa ggcgctaata ccaaacttca gcattttttc gcgcggcatc aacagctgtg
     7621 aattgttttt caccttcacc tcctttgtgg tgctcgcatc ctgtcatagc cgtttcgtta
     7681 aatcgacggc cagttttcaa tcctggaaag cgcctcgcaa agtgaatctc aggtgaaggc
     7741 acgacaacaa aagtattgtg ataaaatcgc caacttctgg tgtcacccta caggaaaaat
     7801 catcatgagc ctggccggta aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta
     7861 taaaacccct gaactggtgc gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat
     7921 gaccgaagcg gcaaaagcct ttatcacccc acttagcttg caggcggttt ctggttatcc
     7981 cgtttccgac agtctgctgg acccggcagc cgaagccgct atgggccata ttgagctggg
     8041 taaatgggct gatttagtga ttctcgcccc tgccacggca gatttgattg cccgtattgc
     8101 tgccggaatg gcgaatgacc tggtatcgac gatttgtctg gctacacctg cgcctgtagc
     8161 cgtgctcccc gccatgaacc agcagatgta ccgtgccgct gccacgcagc ataatttaga
     8221 ggtgcttgct tcccgtggta tgctcatctg ggggccagac agtggcagtc aggcttgtgg
     8281 tgatatcggt cctgggcgaa tgctcgatcc gttaaccatt gtggatatgg cggtggcgca
     8341 tttttcgccc gtcaacgacc tgaaacatct gaacattatg attaccgccg gcccgacgcg
     8401 tgaaccgctc gatccggtgc gttatatctc taatcacagc tccggcaaga tgggttttgc
     8461 tatcgccgcc gccgctgccc gtcgtggcgc gaacgtcacg ctggtatcag gtccggtttc
     8521 actaccgacg ccaccgtttg ttaaacgtgt tgatgtgatg actgcgctgg aaatggaagc
     8581 cgccgtgaat gcttctgtac agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga
     8641 ttatcgcgca gctaccgtgg ccccagagaa aatcaaaaag caggccacgc agggtgatga
     8701 attaacaata aaaatggtta aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga
     8761 ccatcgaccc tacgtcgttg gatttgccgc cgaaacaaat aatgtggaag aatacgcccg
     8821 gcaaaaacgt atccgtaaaa accttgatct gatctgcgcg aacgatgttt cccagccaac
     8881 tcaaggattt aacagcgaca acaacgcatt acaccttttc tggcaggacg gagataaagt
     8941 cttaccgctt gagcgcaaag agctccttgg ccaattatta ctcgacgaga tcgtgacccg
     9001 ttatgatgaa aaaaatcgac gttaagattc tggacccgcg cgttgggaag gaatttccgc
     9061 tcccgactta tgccacctct ggctctgccg gacttgacct gcgtgtctgt ctcgacgacg
     9121 ccgtagaact ggctccgggt gacactacgc tggttccgac cgggctggcg attcatattg
     9181 ccgatccttc actggcggca atgatgctgc cgcgctccgg attgggacat aagcacggta
     9241 tcgtgcttgg taacctggta ggattgatcg attccgacta tcagggccag ttgatgattt
     9301 ccgtgtggaa ccgtggtcag gacagcttca ccattcaacc tggcgaacgc atcgcccaga
     9361 tgatttttgt tccggtagta caggctgaat ttaatctggt ggaagatttc gacgccaccg
     9421 accgcggtga aggcggcttt ggtcactctg gtcgtcagta acacatacgc atccgaataa
     9481 cgtcataaca tagccgcaaa catttcgttt gcggtcatag cgtgggtgcc gcctggcaag
     9541 tgcttatttt caggggtatt ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt
     9601 cgcgaggaaa tacttcagtc tctggcgctg atgctggaat ccagcgatgg aagccaacgt
     9661 atcacgacgg caaaactggc cgcctctgtc ggcgtttccg aagcggcact gtatcgccac
     9721 ttccccagta agacccgcat gttcgatagc ctgattgagt ttatcgaaga tagcctgatt
     9781 actcgcatca atctgattct gaaagatgag aaagacacca cagcgcgcct gcgtctgatt
     9841 gtgttgctgc ttctcggttt tggtgagcgt aatcctggcc tgacccgcat cctcactggt
     9901 catgcgctaa tgtttgaaca ggatcgcctg caagggcgca tcaaccagct gttcgagcgt
     9961 attgaagcgc agctgcgtca ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac
    10021 accaccgatg aaaccctgct ggcaagccag atcctggcct tctgtgaagg tatgctgtca
    10081 cgttttgtcc gcagcgaatt taaataccgc ccgacggatg attttgacgc ccgctggccg
    10141 ctaattgcgg cacagttgca gtaatatgac gccggatgac ttttcatccg gcgagtttct
    10201 ttaaacgcca aactcttcgc gataggcctt aaccgccgcc agatgttccg ccatttccgg
    10261 cttctcttcc aggtaagcaa tcaggtcttt cagggtgatg atagagatca ctttgcagtt
    10321 gtaatcacgc tcaacttcct gaatcgccga aatctcgccg cgcccccgtt cctgacgatc
    10381 aagcgaaatc aacacgccag caagcgtcgc gccattagcc tgaataatct ccatcgattc
    10441 gcgaatcgcc gttccggcgg tgatcacatc atctaccagc attacgcgtc cttgtaacgc
    10501 gctaccaacc agattgccgc cttcaccgtg gtcttttgcc tctttgcggt taaagcagta
    10561 cggcaggtca aggtcatgat gctccgccag cgccacggcg gtagtggtgg caatcgggat
    10621 ccctttgtaa gcagggccaa acagcagatc gaactcaagg ccggaatcca ccaacgcttc
    10681 agcgtaaaaa cggcctaaca gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa
    10741 gaaatagggg cttttgcgcc cggacttcaa cgtaaactcg ccaaacttta acacctgctt
    10801 gccaagcgca aattcaataa actggcgctg atatggtttc atgccttcgc tcctcatctt
    10861 acttttctac agacaaaaaa aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc
    10921 gccgccttct gcgtcgctac aatggattcg attccccctc gggccagagc caacaaggtg
    10981 agtagctctt catgggtgaa cggctcgcct tctgccgtcc cctgcacttc aatgatgcgc
    11041 ccgtcttcgg tcatcactac gttcatgtcg gtctctgcgg cagagtcttc aacgtattcc
    11101 agatcgcaaa ccgcttcgcc gttcacaatt ccgacagaaa ctgcggctac catccctttc
    11161 atcggattgg ttttcagctt gccgttttcc accagcttct gtagcgcatc tgccagcgcc
    11221 acgcaggcac ccgtaatcga cgcggtacgc gtgccaccat cagcctgaag cacgtcgcag
    11281 tccagcgtaa tggtgaactc acccagcgct ttcaaatcta ctgccgcgcg aagagcacgg
    11341 gcgatcagac gctggatttc cattgtgcgt ccaccctgct tacctttcgc cgcttcacga
    11401 gcgttacggg tgtgggtaga acgtggcagc atgccgtact ctgcggtgat ccagccctgg
    11461 ccctgacctt tcaggaagcg cggcacgcct tcttcaatag aggcggtaca caacactttg
    11521 gtatcgccaa attcgaccag caccgagcct tctgcatgtt ttgtatagtt acgagtcagg
    11581 gtaacgggac gcacctgatt attgctacgg cctgctggac gcatattgaa atctccggct
    11641 tgaaacaaat gtggctgcgc attatacgga cttccggcgg ttattcctat cctgacaagg
    11701 catcgatggc tataatcctt ccacctctcc ttttataaac aggaacgtct atgatccgca
    11761 gtatgaccgc ctacgcccgg cgtgaaatca agggtgaatg ggggagcgca acctgggaaa
    11821 tgcgctcggt aaaccagcgt tatctggaaa cttactttcg tctgccggag cagttccgta
    11881 gccttgaacc tgtcgttcgc gagcgtattc gttctcgcct gacgcgcggt aaagtggaat
    11941 gtaccctgcg ctatgagcca gatgttagcg cgcaaggtga gctgatcctc aacgaaaaac
    12001 tggctaaaca gctggtaact gccgcgaact gggtaaaaat gcagagtgac gaaggggaaa
    12061 tcaacccggt tgatattcta cgctggccgg gcgtgatggc agcccaggag caggatcttg
    12121 acgccattgc cgctgaaatt ctcgcggcgc tggatggtac gctggacgac tttattgtcg
    12181 cgcgcgaaac cgaaggtcag gcactgaaag cattgatcga gcagcgtctg gaaggcgtca
    12241 ccgccgaagt ggtcaaagtc cgcgcccata tgccggaaat cctgcaatgg cagcgtgagc
    12301 gtctggtcgc gaagctggaa gatgctcagg ttcagttgga aaacaaccgt ctggagcagg
    12361 agctggttct gctggcacaa cgaattgacg ttgccgaaga actggaccgc ctggaagcgc
    12421 atgtcaaaga gacctacaac attctgaaga aaaaagaagc cgttggtcgt cgtctggact
    12481 ttatgatgca ggaattcaac cgcgagtcga acactcttgc gtcgaagtct atcaatgccg
    12541 aagtgacaaa ctccgcgatt gagctgaaag tgttgattga gcagatgcgc gagcagattc
    12601 agaacattga ataactcgta acgccaattc ttacttttca gccttcacaa atgccgccca
    12661 ctcaaacaga gcggcatttt tcttccccgc aacttcaatt ctgttttgcg tgcctgctcc
    12721 agattttgcg atgttttttt gcccagcaca ctgagaacgt gagatactca caactgtata
    12781 taaatacagt tacagattta ctttctttgc aatcgatatc acatggagtg ggcaatgaac
    12841 gaacatcatc aaccttttga agaaatacgg cattatggca ctgaagggca agaattttgg
    12901 tcagctagag aattggcacc gttactggat tatcgggact ggcgcaattt tcaaaaggta
    12961 ctggcccgcg cgactcaagc gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt
    13021 gaaaccacca aaatggttgt attaggttca ggggctcaac gagaacttga agatgttcat
    13081 ctctcccgct atgcctgtta cctggtagta caaaacggcg accctgcgaa accggtcatt
    13141 gcggcagggc agacttattt tgctatccag acccgacggc aggagcttgc tgacgatgaa
    13201 gcatttaagc aacttcgtga agacgaaaag cgtctgtttc tgagaaatga gttgaaagaa
    13261 cataacaaac aattggttga ggcagcacag taagctgggg tagcgacagc tactgatttc
    13321 gccatatttc agaatcatgg ttaccagggg ctgtatggtg gattagatca gaaagccatc
    13381 caccaactga aggggctgaa aaagagtcag aagatcctgg atcatatggg ttcaacagaa
    13441 ctggcggcta atctctttcg agctacccaa acagaagaaa aactcaagcg ggatggcgtg
    13501 aattcaaaac agcaagctaa taccacacac tttgacgtgg gtagcaaggt gaggcaaacc
    13561 attcaggaac ttggcggaac catgcctgag gagttgccga ccccgcaggt cagcatcaag
    13621 cagttggaaa acagtgtaaa aattacagag aagaaatagc agaaactaaa aactcttttt
    13681 gttgattgag atacccgatg cgtaagccaa agtccaggtg cggtgaacac aatggctaaa
    13741 cattgcacct ttcttttcca cccctcaatt ttaacctatt ttttcttatg cattttctca
    13801 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactacaa
    13861 gttatcaatt tcctctcacc ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac
    13921 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    13981 attactgccg aagccatgac gggggctctg gcggccgggc gacgccgcat ggatacattt
    14041 ggcgtaatta ttattgcgac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    14101 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    14161 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    14221 gtgctcgatg cgcttggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    14281 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc
    14341 gttttgcgcg atatgttctg taaacgtatc ccactggtat tccagaaaga gttgtatgcc
    14401 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    14461 gatgtggtaa ttatctccac cctggtattc ggcttttttg cccgcttact ggcgctacgc
    14521 cttaagctgg gattaccggt cttttactac agccacgaag gacactaagg ttcaaaacct
    14581 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    14641 tcagtaacct gtctggcgcg ccccgatcca gtccccggca gctgctgcca gaactgctcc
    14701 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    14761 agcggtattc ccattgccat cacccagcga gtaaaaggct gcttacgagc cagattaaac
    14821 cgatgccata gctgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    14881 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    14941 cagccggcct caccaatacc atccagcccc agaacctgtt ttgaccccag ccagactaag
    15001 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgagttaaag
    15061 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    15121 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    15181 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    15241 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    15301 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccattctgcc
    15361 tggcctggta gccaatggcg ggattcgggt tctttcgccc ctcgcacaac cacgccatcg
    15421 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    15481 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    15541 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac gcccagagaa
    15601 ttcagcgtgt cgctatcgtc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    15661 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    15721 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    15781 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    15841 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    15901 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    15961 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    16021 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    16081 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    16141 cgtgaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    16201 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    16261 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    16321 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    16381 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    16441 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    16501 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    16561 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    16621 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    16681 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    16741 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    16801 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    16861 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    16921 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    16981 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    17041 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    17101 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    17161 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    17221 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    17281 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    17341 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    17401 agagcaggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    17461 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    17521 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    17581 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    17641 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    17701 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    17761 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    17821 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    17881 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    17941 tcgccgcatc gcccgtgaaa ctctcgaaat ttatagcccg ctggcgcacc gtttaggtat
    18001 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaatcgtta
    18061 ccgcgtaatt aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    18121 aatcctctct gaaatcgaag ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg
    18181 tcgcgaaaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    18241 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattctgaca cctgttatcg
    18301 cgtgctgggc cagatgcaca gcctgtacaa gccgcgtccg ggtcgcgtga aagactatat
    18361 cgccattcct aaagcgaacg gctatcagtc gttgcacacc tcgatgattg gcccgcacgg
    18421 cgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    18481 tgccgcgcac tgggcttata aagagcacgg cgaaaccagt actaccgcac aaatccgcgc
    18541 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    18601 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    18661 gcgcattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    18721 cgatatcggc catgcctgcg tgggcgcacg cgtcgaccgc cagccttacc cgctgtcgca
    18781 gccgcttact agcggtcaga ccgttgaaat cattaccgct ccgggcgctc gcccgaatgc
    18841 cgcttggctg aactttgtcg ttagctcgaa agcgcgcgcc aaaattcgtc agttgctgaa
    18901 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    18961 tggtagccgt aagctcaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    19021 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    19081 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    19141 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    19201 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    19261 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    19321 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    19381 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    19441 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    19501 ccgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    19561 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    19621 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    19681 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    19741 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    19801 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    19861 catgcagatt ctggcaaccc atctttctga taacgctgtc gatttccgcg aaattgatta
    19921 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    19981 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    20041 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    20101 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    20161 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    20221 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    20281 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    20341 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    20401 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    20461 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    20521 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    20581 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    20641 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    20701 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    20761 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    20821 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    20881 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    20941 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    21001 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    21061 aataaactcc tcgccgcctt accgttcaag caaacgggcg cacaggcacg cgtagtggcg
    21121 gagatcgagc gcgatatggc gctggatgtg ccgatgatgc gtctggtgca gggcgatgta
    21181 ggttccggta aaacgctggt cgccgccctc gccgcgttgc gtgcgattgc ccacggcaaa
    21241 caggtagcat tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    21301 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggtaaa
    21361 gcacggctgg cacagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    21421 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    21481 cagcatcgtt ttggcgtaca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    21541 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgact
    21601 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    21661 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    21721 tgcataactg aaggtcgtca ggcatactgg gtttgtacgt tgattgaaga gtcggaattg
    21781 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagagttg
    21841 aacgttggcc tggtacacgg gcggatgaaa cctgccgaga aacaggcggt gatggcgtcg
    21901 tttaaacaag gtgagctaca cctgctggtt gccacaaccg ttattgaagt cggcgttgat
    21961 gtgcctaacg ccagtctgat gattatcgaa aacccggagc gtctgggtct ggcgcagtta
    22021 caccagcttc gcgggcgtgt aggtcgtggt gcggtggctt ctcactgcgt gctgctctac
    22081 aaaacgccgc tctccaaaac cgcgcaaatt cggctgcaag tgctgcgcga cagcaacgac
    22141 ggttttgtga ttgcacagaa agacttagag attcgcggac cgggtgaact gttaggtacg
    22201 cgtcagacgg gtaatgctga gtttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    22261 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca agcaaaagcc
    22321 ctgatagaac gctggatgcc ggagacggaa cgttactcca acgcatgacc agactcatca
    22381 caagacaagt cacgcagtaa atcttcaatc tggatagtga ggatatccat tgcctcatac
    22441 gcatcctgtg caattcgcgc cgccattgcg ccttccggtt caccacgcag aatttcagcc
    22501 gctgcctgta cgccagccac aatcatttca ggtgttaaca tttggcgaag aaaagcgcga
    22561 ttatcagagg accagccatc tttatggcct aatggatgcc cccagcctat ccattcttgc
    22621 gaccataacg gattggcttc tatgggataa attagatgcg cggcatactg aatagcacac
    22681 accgcttttc ctggcgtatc cagcgcccac cagcgcgacc agagagaacg aattaacggc
    22741 gcggccccac ccagagtatt gaacaccgcc agccagcaaa gaacgttatt gaaaccacgc
    22801 tcgttatcca tacgctgcaa cgcggtgtcc agtaaaaatt gccgtacctg ctggcgttgg
    22861 gaagcattca tcatctcctg ccagagatag ggccgacaca atgccttcat taaggattct
    22921 tcgaagtgat caccataacc gccagctaaa acggtttcat gccattgcgc caggcagagc
    22981 ggaaacagat agcgaaacaa ctctatttgc atcggttcgt tataaacgag attgagtaca
    23041 tagtaagcac tcaaatccca ttcgctgatc tgctgccact ctttagccgc cagcgccttg
    23101 agttcggcat ctgcgtaatc aaattccgtt tcccatacac agagcactgg cggctcatca
    23161 ccaaaaatat ggcgacagtg ttcacgtagc gtttccggtt ccttccgacg ctttttcatt
    23221 tttaaccggc aaaaatcggc aacattaaat acagcttaat caccagcgca ttgacgatat
    23281 cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc catgtgcgac gggccaaagc
    23341 gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt tgcaccgagg ccaaaaccac
    23401 agtgacccgc agccagcact gccgcatcgt agtttttgcc catcatgcgc caggtaacga
    23461 agatggcata caacgccatg aagatggtct gtaccaccag aatcgccagc atcggcagcg
    23521 ccagcgaagc cagctcccac agtttcagcc ccatcagcgc catcgccagg aacaacgaca
    23581 ggcttacgtt acccagcacg gataccgcac gctcaaagac gcggtaaaag cccatcattg
    23641 acagaccgtt gctcagaatc acgccaacaa acagtacaca gacgaaggtc ggcagttcaa
    23701 aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac cgtcaggcag atagcaatca
    23761 gcgcgatagt ttcaatcagc accagcgagg tgatcatgcg tcccacatcc ggcttttcaa
    23821 acgccgtcgg gacttcctgg tcatccggaa taccgttcgg cgtggtggag tgtttcacca
    23881 gatagcgcgc caccggaccg ccaatcaagc cgcccagcac cagaccgaac gttgcacagg
    23941 ccatcgccac ttccgtcgca ttggtgaagc cataacgttc aatgaacaat ttactccacg
    24001 cagcgcccgt accgtgaccg ccggaaagcg taatagaacc ggccaacagc cccatcagcg
    24061 gatcaagccc taacaggcta gccataccaa tgccaatggc attttgcatc accaacagac
    24121 caacaaccac aatcaagaag atgccaacca cacgcccacc ggcacgcaaa ctggcaatgt
    24181 tggcgttcag gccaatggtg gcgaagaaag ccagcattaa cggatcgcgc agggacatat
    24241 caaagttgac ttcccagccc atgctttttt tcagtactag tagcgccagc gccaccaaca
    24301 aaccacccgc aacaggttcc ggtatggtgt atttcttcaa aaaggagacg gaatggacca
    24361 acttacgccc gagcagcaac gtcagcgttg cggcaacaag cgttgctaaa gtatcgagat
    24421 gaaacatagt tactcctttg tatccgcatc actctttcat actcgtcata cttcatcgcg
    24481 cagtggcaac ttcatgaact atgacgggta tcgagttatg ctttcctggg tcaaattctc
    24541 agctgttaat gaacaaatgg catggatttt aagcagaaaa ataccaaaag ttatataaaa
    24601 aagtgcattt atctcattta aatatttaga tgaggatgtg gcaaacgttt gcttttctct
    24661 ttcggtcagc taaaatgccc gctttgctac cacgggattg ttttcgatgt ctgtttccac
    24721 cctcgagtca gaaaatgcgc aaccggttgc gcagactcaa aacagcgaac tgatttaccg
    24781 tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc gcctgtcagc atctgctggc
    24841 gatgttcgtt gcggtgatca cgccagcgct attaatctgc caggcgctgg gtttaccggc
    24901 acaagacacg caacacatta ttagtatgtc gctgtttgcc tccggtgtgg catcgattat
    24961 tcaaattaag gcctggggtc cggttggctc cgggctgttg tctattcagg gcaccagctt
    25021 caactttgtt gccccgctga ttatgggcgg taccgcgctg aaaaccggtg gtgctgatgt
    25081 tcctaccatg atggcggctt tgttcggcac gttgatgctg gcaagttgca ccgagatggt
    25141 gatctcccgc gttctgcatc tggcgcgccg cattattacg ccgctggttt ctggcgttgt
    25201 ggtgatgatt atcggcctgt cgctaattca ggttgggtta acgtccattg gcggcggtta
    25261 cgcagccatg agcgataaca ccttcggcgc accgaaaaat ctgctgctgg caggcgtggt
    25321 cttagcctta attatcctgc ttaaccgtca acgtaaccct tacttacgcg tggcctcact
    25381 ggtgattgcg atggcggccg gatatgcgct ggcgtggttt atgggcatgt tgccagaaag
    25441 caacgaaccg atgacgcaag aactgattat ggtgccaacg ccgctctatt acggtcttgg
    25501 cattgaatgg agtctgctgc tgccgctgat gctggtcttt atgatcactt cgctggaaac
    25561 cattggcgat atcacggcga cctctgacgt ttccgaacag ccagtgtccg gtccgctgta
    25621 catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg aactcgtttg tttcggcggt
    25681 gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac ggagtgatcc agttgactgg
    25741 tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg ttgatcgtgc tgggtctgtt
    25801 cccggcagtg agcggttttg tacaacacat tccagaaccg gttctgggcg gcgcaacgct
    25861 tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc gtttctcgtg agccgctgaa
    25921 ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt ggtctgggcg tgtctcagca
    25981 gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac ctgctctcct ccgggatcgc
    26041 cgcgggcggt attactgcca tcgtgctgaa tctgattttc ccaccagaaa aacagtaatt
    26101 cactggcggc ggtaagataa ttaccgccgc agtctcacct ctttcaccat tcccgccttg
    26161 aggattgcgc gtaaatcgtg cataactccc ttatgtgcat ttcacgggat ggaagaccat
    26221 gaaatttatt gggaagctgc ttctctacat tctcatcgct ctgttagtgg tgatcgctgg
    26281 cctctatttt cttctgcaaa cccgctgggg agcagaacat atcagcgcat gggtttccga
    26341 gaatagcgac tatcatctgg ccttcggggc gatggatcac cgtttttccg cgccatctca
    26401 tatcgtgctg gagaacgtca cgtttggtcg tgatggtcag cccgcgaccc tggtggcaaa
    26461 aagtgtcgac attgcgctaa gcagtcggca actgaccgaa ccacgccatg tcgataccat
    26521 cctgctggaa aacgggacgc tgaatctcac cgaccagacc gcgccgctac cgttcaaagc
    26581 cgatcgtctg caactgcgtg atatggcgtt taatagcccg aatagcgaat ggaaactgag
    26641 cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca gaagccggta aagtgctggg
    26701 tacgaaggcg cagattcagt ttagtgccgg atcgctttcg ctcaatgatg ttcctgccac
    26761 caatgtactg attgaaggca gtattgataa cgatcgcgtt acgctgacta acctgggtgc
    26821 cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt aacgccgacg gcagctggca
    26881 agtggaaaat ctgcgcatgg cggatatccg tctacaaagc gaaaaatcgc taaccgactt
    26941 ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt cgcctggaag tgatcgatgc
    27001 tcgtttgcaa ggtccggact gggcggtgac cgacctcgat ctcagcttgc gcaacatgac
    27061 cttcagtaaa gatgactggc agacacaaga aggcaaactg tcgatgaacg ccagcgagtt
    27121 catttatggt tcgctgcatt tatttgaccc gattataaac gcggaatttt ccccgcaggg
    27181 cgtagcgctg cgccagttca ccagccgctg ggaagggggt atggtcagaa cgtcagggaa
    27241 ctggctgcgt gacgggaaaa cgttgatcct tgatgatgcg gcaattgccg ggctggaata
    27301 taccttgccg aaaaactggc aacagttgtg gatggaaacg acacccggtt ggttaaacag
    27361 cctgcaactg aagagattta gcgccagccg caatctgatc attgatatcg accctgactt
    27421 cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac ctgacgctgg ttaccgatca
    27481 taaatggggc gtctggagtg gctcggcgaa tctgaatgcc gccgccgcga cattcaatcg
    27541 tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac agcagcacgg tgaatatcag
    27601 cgaactgagt gcatttactg aaaaaggcat tctggaagcc accgccagtg tttcacaaac
    27661 gccacaacgt cagacacata tcagcctgaa tggacgcggt gtgccggtga atattttgca
    27721 acagtgggga tggcctaaat taccgttgac tggcgacggc aatattcagc ttaccgccag
    27781 tggcgatatt caggccaatg tcccgttgaa acctacggtt agcgggcaat tgcatgccgt
    27841 gaacgccgca aagcagcaag tgactcaaac catgaatgct ggcatcgttt ccagcggtga
    27901 agttacatcg acggagccgg tgcggtaaac atcaatctcg ccgggtacga ctcagacgta
    27961 cccggcattc catcaataga ttacaacgta attgtcagcg cattcccttg aggcttcacc
    28021 accagcccct gctcactttc agcctgcgaa ccgtcttgca gaccatttac tttcacaaca
    28081 ttgcgcaggc acagcgtcca gttcttcgcc tcgcccgcac cagtcacagt aatcgtgttg
    28141 ccagtacgtg ctgcttttaa agtaaagatc accgatccgt cagtagcggg cacttcacag
    28201 acggcttcat gcccgtcttg cagattaaag aggtggaatg ccgtgccttc gtgccacacg
    28261 taatcgggac gttgatcgtt gttgcccagc gccagtagag tgttatcacg cacataaacg
    28321 ggcagactca ggaagccgtg ctgctgttta tgccagcgac taccgtcgag ttcatcgttg
    28381 tgccacaggt gtgtccagcg accttccggc aggtagaact gcacatcgcc cgcttcagtg
    28441 aacaccggcg caaccatcac gttgtcgcct aacatgtatt gacggtcaag gtaatcacaa
    28501 gccggatcgt ccgggaactc catcatcatg gcccgcatca tcggcgtacc ccgcgcgttc
    28561 gcacgcgcag cttcacgata cagatacggc atcatgcggc atttcagttg cgtgaagaag
    28621 cgcaccacat cacaggactc atcatcgtag gcccacggca cacgataaga tttgctaccg
    28681 tgtaaacggc tatggctgga gagcaaacca aacgcgcacc agcgtttgta aacgtgcgcc
    28741 ggagcggtat tttcaaagcc gccgatatcg tggctccaga agccaaaacc tgaaaggcca
    28801 atagacaaac caccgcgcag gctttccgcc attgattcgt agttagcgta acaatcgcca
    28861 ccccagtgta ccgggaattt ctgcgcaccg acggaggccg agcgggcaaa caagacagct
    28921 tcttcctcac caacggtgtc cttgagcacg ttccacacca gttcgttgta gatgtacgca
    28981 taatggttat gcattttctg cggatcggaa ccgtcaaacc actgaacatc agttgggata
    29041 cgttcgccaa agtcggtctt aaagcaatca acgcccatcg cgaccagacc tttcagtttg
    29101 tcggcgtacc atttgcaggc atccggattg gtaaagtcat aaatcgccag acctggctgc
    29161 catttatccc actgccatag cgaaccgtcc gggcgtttga gtaaatagcc tttctcttgt
    29221 aactctttaa agacggggga tttttgaccg atataggggt taatccagac gcagattttc
    29281 agtcctttcg ctttcaggcg gcggatcatc ccttccgggt cagggaaagt cagcgggtcc
    29341 cactcaaaat cgcaccactg gaaggctttc atccagaaac agtcaaagtg gaaaacatgc
    29401 agcggcagat tgcgttccgc cataccatcg ataaagctgt ttaccgtcgc ttcgtcgtag
    29461 ttggtggtaa atgaagtggt tagccacagg ccgaaggacc acgcgggcgg cagcgccgga
    29521 cgaccagtaa agcgggtata acgatcaagt accgctttcg gcgtcgggcc gtcgataaca
    29581 aagtattcga gatattcact ctcaacgctg aactgcactt tggagacttt ctccgatccc
    29641 acttcaaaag agacacactg gggatgattg accagtaccc cataaccacg gttagtcatg
    29701 tagaacggga tatttttata cgcctgttca gtacttgtgc cgccgtcccg gttccaggtc
    29761 tctaccgtct ggccattgcg caccagggca gtaaagcgct ctcccagacc gtaaactgtt
    29821 tcgccaacgc caagatcaag ccgctcaaac atataattgc gttgattatt cgtgtcctgc
    29881 acgtagccat tatttttcac ctgactaccg gtaatacgtt cgccgttgcg cagaaaatcc
    29941 agtgaccaga actcaccttt gctgacacgc gcgcttaagt tgccactttt aaactcagca
    30001 taacgttctg tgttttcgat tgtgaccttc acgtcctgca aaatattgag cggataatga
    30061 ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca caccgacaat accttcctgt
    30121 ggggagaaaa agcgcaacgt aaataaaggc gtatcaagct gccaggtacg ttcacgcaca
    30181 tcacgggggg cagcatagac caccatttca ttatcctgct gttcaacctc gaacacctga
    30241 agcgggtgaa tcaaattgag gccaggttga atcaaccagt ttccatcgct aattttcatt
    30301 gccgttcctt agttctgcac ttcttgagag gtgaattgtt gctggcaacg gcgtttaccc
    30361 tgagccagct gttccataac ggttttcaga ttgtgcgtcg tgagtgagta gtagcgttta
    30421 gcgataatcg cgctcagcaa ataacagatc gccggaacaa tcgtgaatag cgcaataatg
    30481 atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat catatccgcc ataagccagc
    30541 atccagccga taagagcgcc gccgaaggcc aaccccagtt tgagcacaaa cagcgtgcca
    30601 gcaaaactga tcccggtcag gcgtttacca ttgcaccact caccgtagtc gacggtatcg
    30661 gacatcatta cccactggat aggtgtcacc agttgatgca acacaccaat cacgaagatg
    30721 aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga acatcgcgag gctaatcact
    30781 gccagcaggg cgttcgtcca ccagaagata gtgactttac atttccagtc ggtcagaggt
    30841 tttgccagtg cggaaccaat caggttaccc acgcaataag tggtgagaaa agcgacaaac
    30901 acttccggcg tgcccaaaat ccatgtgacg taatacatca tcgccccacc gcgtacgcac
    30961 accgccagga tattgaaaat ggttagtaaa ccgacaatcc gccactggtc gttttgccag
    31021 atatcacgta aatcttcccg catagacgtt gttgtaggtg gtgcttcaac gcgttcttta
    31081 gtggtgaaga aacaaaatgc cagcatcatg aatgccacca cggaaaggac cgcgataccg
    31141 ccctggaaac cgagtggttt attatcaccg ccaattaaat taaccagtgg catcatcaga
    31201 acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac gccaggattg cagcgagata
    31261 cgctgagtcg ggtcattggt gattacacca cccaatgcgc agtaagggat attgacgacg
    31321 gtataaagta aggtaagtag ggtgtaagta attgctgcat agatcatttt gccgttcata
    31381 ctgagatctg gcgtgctata ggccagtaca cagacgatcc cgaatggcag tgcgccaaac
    31441 agtacccacg gacgaaattt accccagcga gagcgcgttc gatcggccaa caaccccatg
    31501 caaggatcgg aaatcgcatc cagtgcacga gcgaccaaaa acatggttcc gacaaaaccg
    31561 gcaggaatgc caaaaatatc ggtataaaag aacatcatat ataacattac gttatcgaaa
    31621 ataatgtggc tggcggcgtc tcccatgcca taaccaattt tctctttaac ggacaacact
    31681 tcactcttca tctgcttttc cttgatgcta taggggtgct gagaccgata acgcattttg
    31741 taaaccatcc ctgtgagagc gaatattccg atatctggtt attaatttat gattcttgtt
    31801 ttatgtgatc gtggtagcgt taatttcgct catatatcat tgtaaaatat aggttttata
    31861 tgaactataa tgctttcgtg ataatacgct gcgtgtatta ggcggaaaaa actgatctgg
    31921 gggatgtaga aactcaagga agtagctata atgcgccccg cctccatgta gcaatcgagg
    31981 cgcggaagat cgtcgtctcc ggtgaggcgg ctggacttca aatccagttg gggccgccag
    32041 cggtcccggg caggttcgac tcctgtgatc ttccgccaaa atgcctctcc tgacgttctc
    32101 caaccccttg taatactgtc aatccagata ttttctactg ctcaatattc aatcggcttc
    32161 aggaatacct ggtctaaggt tttgtgatgg acgatgagcg gctgaagaat ccgcctgtcg
    32221 gttcttctgt cctcgaacgc atccgcgata ttcacgccag tatgcgccgt gtttatttgc
    32281 aggtgatttg cccccctgct gactctcagc caactgtatc gccattaagg tgaagatgtt
    32341 gttttactcg ccccagaaca ccctttttta tacgactatt ctatttcttt aaccgctggc
    32401 gaaaaaaaac tgactataaa cttcaccaat aatcagactg ccatcaagat taatttgttc
    32461 atttaatata tcaaatcaac ttgttttatt tttactatca aaaa
//
LOCUS       DAZFYC010000047        31976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFYC010000047 DAZFYC010000000
VERSION     DAZFYC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.047"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            315..1883
                     /gene="cydA"
                     /locus_tag="ACHKW5_002430"
     CDS             315..1883
                     /gene="cydA"
                     /locus_tag="ACHKW5_002430"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO7511811.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1899..3038
                     /gene="cydB"
                     /locus_tag="ACHKW5_002431"
     CDS             1899..3038
                     /gene="cydB"
                     /locus_tag="ACHKW5_002431"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7511812.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3053..3166
                     /gene="cydX"
                     /locus_tag="ACHKW5_002432"
     CDS             3053..3166
                     /gene="cydX"
                     /locus_tag="ACHKW5_002432"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO7511813.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3166..3459
                     /gene="ybgE"
                     /locus_tag="ACHKW5_002433"
     CDS             3166..3459
                     /gene="ybgE"
                     /locus_tag="ACHKW5_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO7511814.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3609..4013
                     /gene="ybgC"
                     /locus_tag="ACHKW5_002434"
     CDS             3609..4013
                     /gene="ybgC"
                     /locus_tag="ACHKW5_002434"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7511815.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4010..4702
                     /gene="tolQ"
                     /locus_tag="ACHKW5_002435"
     CDS             4010..4702
                     /gene="tolQ"
                     /locus_tag="ACHKW5_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO7511816.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4706..5134
                     /gene="tolR"
                     /locus_tag="ACHKW5_002436"
     CDS             4706..5134
                     /gene="tolR"
                     /locus_tag="ACHKW5_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO7511817.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5199..6464
                     /gene="tolA"
                     /locus_tag="ACHKW5_002437"
     CDS             5199..6464
                     /gene="tolA"
                     /locus_tag="ACHKW5_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO7511818.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKTAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6597..7889
                     /gene="tolB"
                     /locus_tag="ACHKW5_002438"
     CDS             6597..7889
                     /gene="tolB"
                     /locus_tag="ACHKW5_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO7511819.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7924..8445
                     /gene="pal"
                     /locus_tag="ACHKW5_002439"
     CDS             7924..8445
                     /gene="pal"
                     /locus_tag="ACHKW5_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO7511820.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8455..9246
                     /gene="cpoB"
                     /locus_tag="ACHKW5_002440"
     CDS             8455..9246
                     /gene="cpoB"
                     /locus_tag="ACHKW5_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO7511821.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9411..9486
                     /locus_tag="ACHKW5_002441"
     tRNA            9411..9486
                     /locus_tag="ACHKW5_002441"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9444..9446,aa:Lys,seq:ttt)
     gene            9622..9697
                     /locus_tag="ACHKW5_002442"
     tRNA            9622..9697
                     /locus_tag="ACHKW5_002442"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9655..9657,aa:Val,seq:tac)
     gene            9700..9775
                     /locus_tag="ACHKW5_002443"
     tRNA            9700..9775
                     /locus_tag="ACHKW5_002443"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9733..9735,aa:Lys,seq:ttt)
     gene            9925..10000
                     /locus_tag="ACHKW5_002444"
     tRNA            9925..10000
                     /locus_tag="ACHKW5_002444"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9958..9960,aa:Val,seq:tac)
     gene            10004..10079
                     /locus_tag="ACHKW5_002445"
     tRNA            10004..10079
                     /locus_tag="ACHKW5_002445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10037..10039,aa:Lys,seq:ttt)
     gene            10226..10301
                     /locus_tag="ACHKW5_002446"
     tRNA            10226..10301
                     /locus_tag="ACHKW5_002446"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10259..10261,aa:Lys,seq:ttt)
     gene            10335..10410
                     /locus_tag="ACHKW5_002447"
     tRNA            10335..10410
                     /locus_tag="ACHKW5_002447"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10368..10370,aa:Lys,seq:ttt)
     gene            10659..11702
                     /gene="nadA"
                     /locus_tag="ACHKW5_002448"
     CDS             10659..11702
                     /gene="nadA"
                     /locus_tag="ACHKW5_002448"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO7511822.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11740..12459
                     /gene="pnuC"
                     /locus_tag="ACHKW5_002449"
     CDS             11740..12459
                     /gene="pnuC"
                     /locus_tag="ACHKW5_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO7511823.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12456..13397)
                     /gene="zitB"
                     /locus_tag="ACHKW5_002450"
     CDS             complement(12456..13397)
                     /gene="zitB"
                     /locus_tag="ACHKW5_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO7511824.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13511..13891)
                     /gene="ybgS"
                     /locus_tag="ACHKW5_002451"
     CDS             complement(13511..13891)
                     /gene="ybgS"
                     /locus_tag="ACHKW5_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO7511825.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14207..15259
                     /gene="aroG"
                     /locus_tag="ACHKW5_002452"
     CDS             14207..15259
                     /gene="aroG"
                     /locus_tag="ACHKW5_002452"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO7511826.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15417..16169)
                     /gene="gpmA"
                     /locus_tag="ACHKW5_002453"
     CDS             complement(15417..16169)
                     /gene="gpmA"
                     /locus_tag="ACHKW5_002453"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO7511827.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16374..17414)
                     /gene="galM"
                     /locus_tag="ACHKW5_002454"
     CDS             complement(16374..17414)
                     /gene="galM"
                     /locus_tag="ACHKW5_002454"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO7511828.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17408..18556)
                     /gene="galK"
                     /locus_tag="ACHKW5_002455"
     CDS             complement(17408..18556)
                     /gene="galK"
                     /locus_tag="ACHKW5_002455"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO7511829.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18560..19606)
                     /gene="galT"
                     /locus_tag="ACHKW5_002456"
     CDS             complement(18560..19606)
                     /gene="galT"
                     /locus_tag="ACHKW5_002456"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO7511830.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19616..20632)
                     /gene="galE"
                     /locus_tag="ACHKW5_002457"
     CDS             complement(19616..20632)
                     /gene="galE"
                     /locus_tag="ACHKW5_002457"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO7511831.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20893..22365)
                     /gene="modF"
                     /locus_tag="ACHKW5_002458"
     CDS             complement(20893..22365)
                     /gene="modF"
                     /locus_tag="ACHKW5_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO7511832.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDLYRYALTKIN"
     gene            complement(22433..23221)
                     /gene="modE"
                     /locus_tag="ACHKW5_002459"
     CDS             complement(22433..23221)
                     /gene="modE"
                     /locus_tag="ACHKW5_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO7511833.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23350..23499
                     /gene="acrZ"
                     /locus_tag="ACHKW5_002460"
     CDS             23350..23499
                     /gene="acrZ"
                     /locus_tag="ACHKW5_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO7511834.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23665..24438
                     /gene="modA"
                     /locus_tag="ACHKW5_002461"
     CDS             23665..24438
                     /gene="modA"
                     /locus_tag="ACHKW5_002461"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7511835.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24438..25127
                     /gene="modB"
                     /locus_tag="ACHKW5_002462"
     CDS             24438..25127
                     /gene="modB"
                     /locus_tag="ACHKW5_002462"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO7511836.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSILHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25130..26188
                     /gene="modC"
                     /locus_tag="ACHKW5_002463"
     CDS             25130..26188
                     /gene="modC"
                     /locus_tag="ACHKW5_002463"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO7511837.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26189..27007)
                     /gene="ybhA"
                     /locus_tag="ACHKW5_002464"
     CDS             complement(26189..27007)
                     /gene="ybhA"
                     /locus_tag="ACHKW5_002464"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO7511838.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27162..28157
                     /gene="pgl"
                     /locus_tag="ACHKW5_002465"
     CDS             27162..28157
                     /gene="pgl"
                     /locus_tag="ACHKW5_002465"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO7511839.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28198..29151)
                     /gene="ybhD"
                     /locus_tag="ACHKW5_002466"
     CDS             complement(28198..29151)
                     /gene="ybhD"
                     /locus_tag="ACHKW5_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7511840.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKSLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            29335..30387
                     /gene="ybhH"
                     /locus_tag="ACHKW5_002467"
     CDS             29335..30387
                     /gene="ybhH"
                     /locus_tag="ACHKW5_002467"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO7511841.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30463..31896
                     /gene="ybhI"
                     /locus_tag="ACHKW5_002468"
     CDS             30463..31896
                     /gene="ybhI"
                     /locus_tag="ACHKW5_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO7511842.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         7681 a         7804 c         8423 g         8068 t
ORIGIN      
        1 gcatcaaaaa gagataaatt gttctcgatc aaattggctg aaaggcggta atttagctat
       61 aaattgatca ccgtcgaaaa atgcaaattt gcttcaacaa aaacctgttt attgtaagga
      121 ttttgcggcg taatatatac gtgggatcaa tttgagtttt tattaacatg tttgcaacct
      181 ttctttacgc cgtttttgtg tgcattcaca tggtatgatg aaagtgttca aacaaatttc
      241 tattggggca tgcgtgtgac cctttctaac ggggttcact ctcggagtct tcatgcgatg
      301 agcaaggagt catgatgtta gatatagtcg aactgtcgcg cttacagttt gccttgaccg
      361 cgatgtacca cttccttttt gtgccactga cgctcggtat ggcgttcctg ctggccatta
      421 tggaaacggt ctacgtcctc tccggcaaac agatttataa agatatgacc aagttctggg
      481 gcaagttgtt tggtatcaac ttcgctctgg gtgtggctac cggtctgacc atggagttcc
      541 agttcgggac taactggtct tactattccc actatgtagg ggatatcttc ggtgcgccgc
      601 tggcaatcga aggtctgatg gccttcttcc tcgaatccac ctttgtaggt ctgttcttct
      661 tcggttggga tcgtctgggt aaagttcagc atatgtgtgt cacctggctg gtggcgctcg
      721 gttcaaacct gtccgcactg tggattctgg ttgcgaacgg ctggatgcaa aacccaatcg
      781 cgtccgattt caactttgaa actatgcgta tggagatggt gagcttctcc gagctggtgc
      841 ttaacccggt tgctcaggtg aaattcgttc acactgtagc gtctggttat gtgactggcg
      901 cgatgttcat cctcggtatc agcgcatggt atatgctgaa aggtcgtgac ttcgccttcg
      961 ctaaacgctc ctttgctatc gctgccagct tcggtatggc tgctgttctg tctgttattg
     1021 ttctgggtga tgaatccggc tacgaaatgg gcgacgtgca gaaaaccaaa ctggctgcta
     1081 ttgaagccga gtgggaaacg caacctgcgc ctgctgcctt tactctgttc ggcattcctg
     1141 atcaggaaga ggagacgaac aaatttgcga ttcagatccc ttacgcactg ggcatcattg
     1201 caacgcgttc cgtggatacc ccggttatcg gcctgaaaga gctgatggtg cagcatgaag
     1261 aacgcattcg taacgggatg aaggcgtact ctctgctcga acaactgcgt tctggttcta
     1321 ccgaccaggc ggttcgtgac cagttcaata gcatgaagaa agacctcggt tacggtctgc
     1381 tgctgaaacg ctatacgcca aacgtggctg atgcgactga agcgcagatt caacaggcaa
     1441 ccaaagactc catcccgcgt gtagcgccgc tgtactttgc gttccgtatc atggtggcgt
     1501 gtggcttcct gcttctggca atcatcgcgc tctctttctg gagtgtcatc cgcaaccgca
     1561 ttggcgagaa aaaatggctt ctgcgcgccg cgctgtacgg tattccgctg ccgtggattg
     1621 ctgtagaagc gggctggttc gtggctgaat atggccgcca accgtgggct atcggtgaag
     1681 tgctgccgac agctgtggcg aactcgtcac tgaccgcagg cgatctcatc ttctcaatgg
     1741 tgctgatttg cggcctgtat accctgttcc tggtggcaga attgttctta atgttcaaat
     1801 ttgcacgcct cggcccaagc agcctgaaaa ccggtcgcta tcactttgag cagtcttcca
     1861 cgactactca gccggcacgc taagacagga gtcgtcaaat gatcgattat gaagtattgc
     1921 gttttatctg gtggctgctg gttggcgttc tgctgattgg ttttgcagtc actgacggtt
     1981 tcgacatggg ggtgggcatg ctcacccgtt tcctcggtcg taacgacacc gagcgtcgaa
     2041 ttatgattaa ctccattgca ccacactggg acggtaacca ggtttggctg atcaccgcgg
     2101 gcggcgcact ctttgctgcc tggccgatgg tctatgccgc tgcgttctcc ggcttctatg
     2161 tggcgatgat cctcgtgctg gcgtctttgt tcttccgtcc ggtcggtttt gactaccgct
     2221 ccaagattga agaaacccgc tggcgtaaca tgtgggactg gggcatcttc attggtagct
     2281 tcgttccgcc gctggtaatt ggtgtagcgt tcggtaacct gttgcagggc gtaccgttca
     2341 acgttgatga atatctgcgt ctgtactaca ccggtaactt cttccagttg cttaacccgt
     2401 tcggcctgct ggcaggcgtg gtgagcgtag ggatgatcat tactcagggc gcaacctatc
     2461 tgcaaatgcg taccgtgggc gaactgcacc tgcgtacccg tgcaacggct caggtggctg
     2521 cgctggtgac actggtctgt ttcgcactgg ctggcgtatg ggtgatgtac ggtatcgatg
     2581 gttatgtcgt gaaatcgaca atggaccatt acgcagcctc taacccactg aataaagaag
     2641 tggttcgtga agctggcgca tggctggtta acttcaacaa cacgccaatt ctgtgggcta
     2701 ttccggcact gggtgtggtt ctgccgctgc tgaccatcct gactgcacgt atggataaag
     2761 ccgcgtgggc gtttgtgttc tcctccctga cgctggcctg catcatcctg acagccggta
     2821 tcgcaatgtt cccgtttgtg atgccgtcca gcaccatgat gaacgcaagt ctgacaatgt
     2881 gggatgcaac ttccagccag ctgacgctta acgtcatgac ctgggttgcg gtggttctgg
     2941 taccgatcat tctgctctac accgcctggt gttactggaa aatgttcggt cgtatcacca
     3001 aagaagatat tgaacgtaac acccactctc tgtactaagt aaggagctaa aaatgtggta
     3061 tttcgcatgg attctgggaa cgcttcttgc ctgttcgttt ggggtaatca ccgcgctggc
     3121 gcttgaacac gtcgaatcag gcaaagccgg tcaagaagac atctgatgag taagattatc
     3181 gcgactttgt atgcggtaat ggacaagcgc cccctgcggg cgctttcctt cgtgatggcg
     3241 cttctgttag caggatgtat gttttgggac ccatctcgtt ttgccgcgaa gaccagtgaa
     3301 ctggaaatct ggcatggttt attgctgatg tgggccgtct gtgctggtgt gattcacggc
     3361 gtgggctttc gtccgcagaa ggttctttgg caagggattt tttgcccatt gcttgccgat
     3421 attgttctca ttgtcgggct gattttcttc ttcttttaaa tcagaattct ctttaaaaaa
     3481 ttatgggccg ctccaggccc ataaattttt acgctccctt aacttgccct cattcccaaa
     3541 cctcaatcgc gcgcgtatag tagcagcgtt taaaagctct aacttttgtt gcattaccgg
     3601 gatgtaaagt gaatacaacg ctgtttcgat ggccggttcg cgtctactat gaagataccg
     3661 atgccggtgg tgtggtgtac cacgccagtt acgtcgcttt ttatgaaaga gcacgcacag
     3721 agatgctgcg tcatcatcac ttcagtcagc aggcgctgat ggctgaacgc gttgcctttg
     3781 tggtacgtaa aatgacggtg gaatattacg cacctgcgcg gctcgacgat atgctcgaaa
     3841 tacagactga aataacatca atgcgtggca cctctttggt tttcacgcaa cgtattgtca
     3901 acgccgagaa tactttgctg aatgaagcag aggttctggt tgtttgcgtt gacccactca
     3961 aaatgaagcc tcgtgcgctt cccaagtcta ttgtcgcgga gtttaagcag tgactgacat
     4021 gaatatcctt gatttgttcc tgaaggctag ccttctggtt aaacttatca tgttgatttt
     4081 gattggtttt tcaatcgcat cttgggccat tattatccag cggacccgta ttcttaacgc
     4141 agcggcgcgc gaagccgaag cgtttgaaga taaattctgg tctggaatcg aactctctcg
     4201 cctctatcaa gagagccagg ggaaacggga taatctgact ggttcggaac aaatctttta
     4261 cagcgggttc aaagagtttg tgcgcctgca tcgtgccaat agccatgcgc cggaagccgt
     4321 agtggaaggg gcgtcgcgtg ctatgcgtat ctccatgaac cgtgaacttg aaaatctgga
     4381 aacgcacatt ccgttcctcg gtacggttgg ctccatcagc ccgtatattg gtctgtttgg
     4441 tacggtctgg gggatcatgc acgcctttat cgccctcggg gcggtaaaac aagcaacact
     4501 gcaaatggtt gcgcccggta tcgcagaagc gttgattgcg actgcaattg gtctgttcgc
     4561 cgcaattccg gcggtaatgg cttataaccg cctcaaccag cgcgtaaaca aactggaact
     4621 gaattacgac aactttatgg aagagtttac cgcgattctg caccgccagg cgtttaccgt
     4681 tagcgagagc aacaaggggt aagccatggc cagagcgcgt ggacgaggtc gtcgcgatct
     4741 caagtccgaa atcaacattg taccgttgct ggacgtactg ctggtgctgt tgctgatctt
     4801 tatggcgaca gcgcccatca tcacccagag cgtggaggtc gatctgccag acgctactga
     4861 atcacaggcg gtgagcagta acgataatcc gccagtgatt gttgaagtgt ctggtattgg
     4921 tcagtacacc gtggtggttg agaaagatcg cctggagcgt ttaccaccag agcaggtggt
     4981 ggcggaagtg tccagccgtt tcaaggccaa cccgaaaacg gtctttctga tcggtggcgc
     5041 aaaagatgtg ccttacgatg aaataattaa agcactgaac ttgttacata gtgcgggtgt
     5101 gaaatcggtt ggtttaatga cgcagcctat ctaaacatct gcgtttccct tgcttgaaag
     5161 agagcgggta acaggcgaac agtttttgga aaccgagagt gtcaaaggca accgaacaaa
     5221 acgacaagct caagcgggcg ataattattt cagcagtgct gcatgtcatc ttatttgcgg
     5281 cgctgatctg gagttcgttc gatgagaata tagaagcttc agccggaggc ggcggtggtt
     5341 cgtccatcga cgctgtcatg gttgattcag gtgcggtagt tgagcagtac aaacgcatgc
     5401 aaagccagga atcaagcgcg aagcgttctg atgaacagcg caagatgaag gaacagcagg
     5461 ctgctgaaga actgcgtgag aaacaagcgg ctgaacagga acgcctgaag caacttgaga
     5521 aagagcggtt agcggctcag gagcagaaaa agcaggctga agaagccgca aaacaggccg
     5581 agttaaagca gaagcaagct gaagaggcgg cagcgaaagc ggcggcagat gctaaagcga
     5641 aggccgaagc agatgctaaa gctgcggaag aagcagcgaa gaaagcggct gcagacgcaa
     5701 agaaaaaagc agaagcagaa gccgccaaag ccgcagccga agcgcagaaa aaagccgagg
     5761 cagccgctgc ggcactgaag aagaaagcgg aagcggcaga agcagctgca gctgaagcaa
     5821 gaaagaaagc ggcaactgaa gctgctgaaa aagccaaagc agaagctgag aagaaagcgg
     5881 ctgctgaaaa ggctgcagct gataagaaag cggcagcaga gaaagctgca gccgacaaaa
     5941 aagcagcaga aaaagcggct gctgaaaagg cagcagctga taagaaagca gcggcagaaa
     6001 aaaccgccgc agacaaaaaa gcggcagcgg caaaagctgc agctgaaaaa gccgctgcag
     6061 caaaagcggc cgcagaggca gatgatattt tcggtgagct aagctctggt aagaatgcac
     6121 cgaaaacggg gggaggggcg aaagggaaca atgcttcgcc tgccgggagt ggtaatacta
     6181 aaaacaatgg cgcatcaggg gccgatatca ataactatgc cgggcagatt aaatctgcta
     6241 tcgaaagtaa gttctatgac gcatcgtcct atgcaggcaa aacctgtacg ctgcgcataa
     6301 aactggcacc cgatggtatg ttactggata tcaaacctga aggtggcgat cccgcacttt
     6361 gtcaggctgc gttggcagca gctaaacttg cgaagatccc gaaaccacca agccaggcag
     6421 tatatgaagt gttcaaaaac gcgccattgg acttcaaacc gtaatcgcga tgttgactgt
     6481 tcggacggtc aacatcaggc accggttgcc acggggttct ggtagttttg tgtattttag
     6541 tttgttaaca ttctgctaaa ttatcgtggg ccatcggtcc agataaggga gatatgatga
     6601 agcaggcatt acgagtagca tttggttttc tcatactgtg ggcatcagtt ctgcatgctg
     6661 aagtccgcat tgtgatcgac agcggtgtag attccggtcg tcctattggt gttgttcctt
     6721 tccagtgggc ggggcctggt gcggcacctg aagatattgg cggcatcgtt gctgctgact
     6781 tgcgtaacag cggtaaattt aatccgttag atcgcgctcg tctgccacag cagccgggta
     6841 gtgcgcagga agtacaacca gctgcatggt ccgcactggg cattgacgct gtagttgtcg
     6901 gtcaggtcac tccgaatccg gatggttctt acaatgttgc ttatcaactt gttgacactg
     6961 gcggcgcacc gggtactgta cttgctcaga actcgtacaa agtgaacaag cagtggctgc
     7021 gttatgctgg tcataccgcc agtgatgaag tgtttgaaaa actgaccggc attaaaggtg
     7081 cgttccgtac tcgtattgcc tacgttgttc agaccaacgg cggtcagttc ccgtatgaac
     7141 tgcgcgtatc tgactatgac ggttacaacc agtttgtcgt tcaccgttca ccgcagccgc
     7201 tgatgtctcc ggcgtggtca ccagacggtt ctaaactggc ttatgtgacc ttcgaaagcg
     7261 gtcgttccgc gctggttatt cagacgctgg caaatggcgc tgtacgtcag gtggcttcat
     7321 tcccgcgtca caacggtgca cctgcattct cgccagacgg cagcaaactg gcattcgcct
     7381 tgtcgaaaac cggtagtctg aacctgtacg taatggattt ggcttctggt cagatccgcc
     7441 aggtgactga tggtcgcagt aacaataccg aaccgacctg gttcccggat agccagaacc
     7501 tggcatttac ttctgaccag gccggtcgtc cgcaggttta taaagtgaat atcaacggcg
     7561 gtgcgccaca acgtattacc tgggaaggtt cgcagaacca ggatgcggat gtcagcagcg
     7621 acggtaaatt tatggtaatg gtcagctcca atggtgggca gcagcacatt gccaaacaag
     7681 atctggcaac gggaggcgta caagttctgt cgtccacgtt cctggatgaa acgccaagtc
     7741 tggcacctaa cggcactatg gtaatctaca gctcttctca ggggatggga tccgtgctga
     7801 atttggtttc tacagatggg cgtttcaaag cgcgtcttcc ggcaactgat ggacaggtca
     7861 aattccctgc ctggtcgccg tatctgtgat aataattaat tgaatagtaa aggaatcatt
     7921 gaaatgcaac tgaacaaagt gctgaaaggg ctgatgattg ctctgcctgt tatggcaatt
     7981 gcggcatgtt cttccaacaa gaacgccagc aatgacggca gcgaaggcat gctgggtgcc
     8041 ggcactggta tggatgcgaa cggcggcaac ggcaacatgt cttccgaaga gcaggctcgt
     8101 ctgcaaatgc aacagctgca gcagaacaac atcgtttact tcgatctgga caagtacgat
     8161 atccgttctg acttcgctca aatgctggat gcacatgcaa acttcctgcg tagcaacccg
     8221 tcttacaaag tcaccgtaga aggtcacgcg gacgaacgtg gtactccgga atacaacatc
     8281 tccctgggtg aacgtcgtgc gaacgccgtt aagatgtacc tgcagggtaa aggcgtttct
     8341 gcagaccaga tctccatcgt ttcttacggt aaagaaaaac ctgcagtact gggtcatgac
     8401 gaagcggcat actccaaaaa ccgtcgtgcg gtactggttt actaagagaa ttgcatgagc
     8461 agtaacttca gacatcaact attgagtctg tcgttactgg ttggtatagc ggccccctgg
     8521 gccgcttttg ctcaggcacc aatcagtagt gtcggctcag gctcggtcga agaccgcgtc
     8581 actcaacttg agcgtatttc taatgctcac agccagcttt taacccaact ccagcaacaa
     8641 ctttctgata atcaatccga tattgattcc ctgcgtggtc agattcagga aaatcagtat
     8701 caactgaatc aggtcgtgga gcggcaaaag cagatcctgt tgcagatcga cagcctcagc
     8761 agcggtggtg cagcggcgca atcaaccagc agcgatcaaa gcggtgcggc ggcatcaacg
     8821 acgccgacag ctgatgctgg tactgcgaat gctggcgcgc cggtgaaaag cggcgatgca
     8881 aacacggatt acaatgcagc tattgcgctg gtgcaggata aatcccgcca ggatgacgca
     8941 atggtggcat ttcagaattt catcaaaaat taccctgatt caacttacct gccaaacgcc
     9001 aattattggc tgggtcagtt aaactacaac aagggtaaaa aagatgatgc ggcgtactat
     9061 tttgcttcgg tagtgaaaaa ctatccgaag tcaccaaagg ctgctgatgc gatgtttaaa
     9121 gtcggcgtca tcatgcagga caaaggtgac accgcaaaag cgaaagccgt ataccagcag
     9181 gttatcagta aataccctgg tactgatggc gctaaacagg cacaaaaacg tctgaacgcg
     9241 atgtaatgca taacacacga ccagaagtcg cattatttct ggtcgtgtcg tgcgaatcat
     9301 aagcagttga gtgatctgca tcgaaatttt tgttgcgctc aagtctgaaa tcagtaatat
     9361 atgccgccgt tgccacggga tatcaaacaa accgaaagca acgaaaaagt gggtcgttag
     9421 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
     9481 ccaccaatcg ctaaggtgga agcggtagta aaacgtgaag gataacgttg catcagcaac
     9541 ggcccgaagg gcgagacgaa gtcgagtcat cctgcacgac ccaccactaa catagttagt
     9601 tgtagtatcc agcgtagtat tgggtgatta gctcagctgg gagagcacct cccttacaag
     9661 gagggggtcg gcggttcgat cccgtcatca cccaccaccg ggtcgttagc tcagttggta
     9721 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccagtttt
     9781 agcatcgaag acagatgtta agcgtgtagg ataacgttgc gtcagcaacg gcccgtaggg
     9841 cgagcgaagc gagtcatcct gcacgaccca ccactaatta cggtgggttc ggtggaagta
     9901 gtttgtagta tccagcgcag tatcgggtga ttagctcagc tgggagagca cctcccttac
     9961 aaggaggggg tcggcggttc gatcccgtca tcacccacca ctcgggtcgt tagctcagtt
    10021 ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccaa
    10081 ttttaacatc aaactcagat gttaagcgtg aaggataacg ttgcgccagc aacggcccgt
    10141 agggcaagcg aagcgagtca tcctgcacga cacaccaatc ttaaagattg gccccgagta
    10201 gaaatctttc aggtaacacc cgtatgggtc gttagctcag ttggtagagc agttgacttt
    10261 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc atcctgaatg attaaagcag
    10321 cataatcccg caaggggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg
    10381 tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt
    10441 tgctattcag gcatcctcaa tttcactttg taaacctgat gacatcgtca gagcttactg
    10501 tgcaagcaac tctacgtcgg tggaattagg cgtaattcga gtgacttttc cccaccattc
    10561 gactatcttg tttagcatat aaaacaaatt acaccgataa cagcgaatat tacgctaatg
    10621 tcggttttaa cgttaagcct gtaaaacgag atggtaagat gagcgtaatg tttgatccag
    10681 acacggcgat ttatcctttc cccccgaagc cgacgccgtt aagcattgat gaaaaagcgt
    10741 attaccgcga gaagataaaa cgtctgctaa aagaacgtaa tgcggtgatg gttgcccact
    10801 actataccga tcccgaaatt caacaactgg cagaagaaac cggtggctgt atttctgatt
    10861 ctctggaaat ggcgcgcttc ggtgcaaagc atcccgcttc tactttgtta gtcgctgggg
    10921 tgagatttat gggagaaacc gccaaaattc tcagtccgga aaaaacaatt ctgatgccga
    10981 cacttcaggc tgaatgttca ctggatctcg gctgccctgt tgaagaattt aacgcatttt
    11041 gcgatgccca tcccgatcgt actgtcgtcg tctacgccaa cacttctgct gcggtaaaag
    11101 cgcgcgcaga ttgggtggta acttcaagca ttgccgtcga acttattgat catcttgata
    11161 gtttgggtga aaaaatcatc tgggcacccg acaaacatct ggggcgttac gtgcaaaaac
    11221 agacgggtgg agacattcta tgctggcagg gtgcctgtat tgtgcatgat gaatttaaga
    11281 ctcaggcgtt aacccgcttg caagaagaat acccggatgc tgccatactg gtgcatccag
    11341 aatcaccaca agctattgtc gatatggcgg atgcggtcgg ttccaccagt caactgatcg
    11401 ctgctgcgaa aacattgcca catcagcggc ttatcgtggc aaccgatcgg ggcattttct
    11461 acaaaatgca gcaggcagtg ccagataaag agttactgga agcaccaacc gcaggtgagg
    11521 gtgcaacctg ccgcagctgc gcgcattgtc cgtggatggc catgaatggc cttcagtcca
    11581 tcgcagaggc attagaacag gaaggaagca atcacgaggt tcatgttgat gaaaggctgc
    11641 gagagagggc gctggtgccg ctcaatcgta tgctggattt tgcggctaca ctacgtggat
    11701 aacgaataat aaggcgtaac gttacgcttt gggggaaaga tggatttttt tagtgtgcag
    11761 aatatcctgg tacatatacc aataggggca ggcggttatg atctctcatg gatcgaagcg
    11821 gtaggcacga tcgccgggtt gctgtgtatt ggccttgcca gtctggagaa gatcagcaac
    11881 tacttctttg gcctgatcaa cgtcaccttg tttggcatta ttttctttca gattcagctg
    11941 tatgccagcc tgctattaca ggtgtttttc tttgccgcga atatttacgg ttggtatgcg
    12001 tggtcgcgac aaaccagtca gaacgaggcg gagttgaaaa ttcgctggtt gccattgccg
    12061 aaggcactca gctggttggc ggtttgcgtt gtttcgattg gtctgatgac ggtatttatc
    12121 aatccggtgt ttgcattttt gacccgcgtg gcagtcatga tcatgcaagc attaggatta
    12181 caggttgtga tgcctgaact gcaaccggac gctttcccgt tctgggattc atgcatgatg
    12241 gtgttatcta tcgtggcaat gattctgatg acgcgtaagt atgtggaaaa ctggctgttg
    12301 tgggtgatta ttaacgtgat tagcgtcgtt atttttgcac ttcagggcgt ttacgccatg
    12361 tctctggagt acatcatcct gacctttatt gcgctcaacg gcagccggat gtggatcaac
    12421 agcgcacgtg aaagaggctc acgcgcgctg tcccattaat ggtgatgatg tgaatggcca
    12481 gacacccctt cattcaggtg gcagtctggc ccatgacaag gttgatattc catctgaatg
    12541 gtggcgtgct caatctgata gtgatccatc aggtagtgtt ggatctgatc caacaaggca
    12601 tcgtgatcgt gtggcgggat cacctgcaca tgcaacgtca tcaccggctt ctcgcctacc
    12661 atccatacat gtacatggtg cacattgcgg acttccggga tttcccggca catacgacgc
    12721 ttcagttcag cgatatccag cgataccggt gcaccttcaa gtaattcatt cacgctatct
    12781 ttcaacagtc gccacgcgct acgcagaacc agaagcgaca ctagtatcga gagaatgggg
    12841 tcagcaggtg tccagccagt ccagataata atcaacgcgg caataatagc cccaaccgaa
    12901 cccagcaggt ccccgagcac atgcagtgcc gctgctcgta cgttgaggtt tttctcttcg
    12961 ctgccgtgat gaagtaacca gaaagaaagt atatttgcca gcaacccagc cacggcaatt
    13021 gccatcatca tgccgccctc gaccggacgc ggcgtgcgga agcgttctat cgcctcccag
    13081 acaatcaaaa tggtaattac cacgagggct atggcattca caaacgccgc gagcgtggtc
    13141 agtcttagcc aaccgaaagt gtggcgaatg gtgggaggac gacgtgaaaa ttgcacggcg
    13201 agtagggcga aaagcagggc ggcagtatcg gtcaacatat gacccgcatc ggccagcaat
    13261 gccagagaac cagaaagaaa accaccaacg acttctacca gcataaaccc ggcagtcacg
    13321 ccgaaagcat acaacaagcg acgagcatta ttatcttctg gcaggtgtga ggacgtgtgt
    13381 gagtgtgagt gcgccatgac gagtcatccc ttaattattc tcacttaact ttatgacatc
    13441 atactgcttt tagaagtgaa aaattaaaag ggagagactc cgctctccca ttattggcta
    13501 ttttgcaggg ttactgcgtg gtaccgtcgg ttttggtatc gacatcatta ttgatgccat
    13561 caccggtttg taccttttta ttgatatccg gacagcgacc atctttgcac atggtgttct
    13621 tgtgctcttc atctttggtc attccgtcat tgttcattga agaaccatcc gaatgcagca
    13681 ttgtgccacc agaaccggta tttaccccgt tattgtcgac gttatttggc gcgacatttt
    13741 cacgggcgtc aggggctacc tggcccgcat cagctgcggc gtttgcctgg ccgttattag
    13801 tttgcgctcc gctatcggca gccagtgcgg caccgctggc aaggcttaga gtggcagtca
    13861 gaaataatgt ggccagtttt gtcattttca taggatgctc ctgttatggt cgttatgtcg
    13921 gataacctct tccaacagtg catttgcagg tgaatataag gcattggttt aagatttcag
    13981 ccaggttatg aaacgcagca gagaatcttg aaataattaa caaacaaagg agttacagtt
    14041 agaaattgta ggagagatct cgtttttctc gacaatctgg cgtttttctt gctaattcca
    14101 ggattaatcc gttcatagtg taaaaccccg tttacacatt ctgacggaag atatagattg
    14161 gaagtattgc attcactaag ataagtatgg caacactgga acagacatga attatcagaa
    14221 cgacgattta cgcatcaaag aaatcaaaga gttacttcct cctgtcgcat tgctggaaaa
    14281 attccccgct actgaaaatg ccgcgaatac ggttgcccat gcccgaaaag cgatccataa
    14341 gatcctgaaa ggtaatgatg atcgcctgtt ggttgtgatt ggcccatgct caattcatga
    14401 tcctgtcgcg gcaaaagagt atgccactcg cttgctggcg ctgcgtgaag agctgaaaga
    14461 tgagctggaa atcgtaatgc gcgtctattt tgaaaagccg cgtaccacgg tgggctggaa
    14521 agggctgatt aacgatccgc atatggataa tagcttccag atcaacgacg gtctgcgtat
    14581 agcccgtaaa ttgctgcttg atattaacga cagcggtctg ccagcggcag gtgagtttct
    14641 cgatatgatc accccacaat atctcgctga cctgatgagc tggggcgcaa ttggcgcacg
    14701 taccaccgaa tcgcaggtgc accgcgaact ggcatcaggg ctttcttgtc cggtcggctt
    14761 caaaaatggc accgacggta cgattaaagt ggctatcgat gccattaatg ccgccggtgc
    14821 gccgcactgc ttcctgtccg taacgaaatg ggggcattcg gcgattgtga ataccagcgg
    14881 taacggcgat tgccatatca ttctgcgcgg cggtaaagag cctaactaca gcgcgaagca
    14941 cgttgctgaa gtgaaagaag ggctgaacaa agcaggcctg ccagcacagg tgatgatcga
    15001 tttcagccat gctaactcgt ccaaacaatt caaaaagcag atggatgttt gtgctgacgt
    15061 ttgccagcag attgccggtg gcgaaaaggc cattattggc gtgatggtgg aaagccatct
    15121 ggtggaaggc aatcagagcc tcgagagcgg ggagccgctg gcctacggta agagcatcac
    15181 cgatgcctgc atcggctggg aagataccga tgctctgtta cgtcaactgg cgaatgcagt
    15241 aaaagcgcgt cgcgggtaag gtttaattgt cggatgcgcc gtcagagtgg cgtatccgat
    15301 gaatcaccac aggcctgata agtcgcgcag cgtcgcatca ggcaatgtgc tccattgtta
    15361 gcaacaaaaa tgccgactca cttgcagtcg gctttctcat tttaaacgaa tgacgtttac
    15421 ttcgctttac cctggtttgc aaccgccgct gctttcgctg cgatctcgtc agcattaccc
    15481 agataatagc gtttcagcgg tttgaaattc tcgtcgaact catacaccag cggcacgcca
    15541 gtcgggatat taagctcaag aatctcttct tcgctcatgt tatcaagata tttcaccagc
    15601 gcacgtaaag agttaccgtg tgcagcgatg atcacgcgct caccgctctt catacgcggc
    15661 agaatagttt cattccagta agggatcacg cggtcaatgg tcagcgccag gctttccgtc
    15721 agcggcagtt ctttttcgct cagtttcgcg taacgcggat cgtgacccgg ataacgctca
    15781 tcatctttag tcagttccgg cggagtcact gcaaaaccac gacgccactg tttcacctgc
    15841 tcgtcgccat acttttcagc agtttccgct ttgttcagac cctgcaacgc accgtagtga
    15901 cgttcgttca gtttccagga tttctcaacg ggcagccatg cctgatccag ttcgtccagc
    15961 acattccaca gggtatggat agcgcgtttc agcacagagg tgtaagcaaa gtcaaagctg
    16021 taaccttcct ctttcagcag cttacctgct gcttttgctt cgcttacgcc tttctcagac
    16081 agatccacgt cgtaccaacc ggtgaaacgg ttttctttgt tccactgact ttcgccatga
    16141 cgaaccagaa ccagcttagt tacagccata tacttactcc tcaaatcatt ttttaatgat
    16201 aataattctc attatattgc cgtgacgaag caacagcaat gcttacgcat aaccatagcg
    16261 aaaatagtgt cgcagtgtaa ggttgttgtg aatattgagt tgcaaatatg tcggtgtttg
    16321 ctggtgattt gagcaatata aggataaagc cctcattacg agggcttcag gtgctactca
    16381 gcaataaact gatattccgt caggctggaa tactcttcgc caggacgcag gaagcagtcc
    16441 ggttgcggcc attcagggtg gtttgggctg tccggcagaa actcgctttc cagtgccagg
    16501 ccttgccagt cggcgtaagg ttcagttcct cttgatggcg tgccgccgag gaagttgccg
    16561 gagtagaatt gcagagccgg agcggtggtg tagaccttca gctgcaattt ttcatctgct
    16621 gaccagacat gcgccgccac tttcttgcca tcgcctttgg cctgtaacaa gaatgcgtga
    16681 tcgtaacctt tcactttgcg ctgatcgtcg tcggcaagaa actcactggc gatgattttg
    16741 gcgctacgga aatcaaaaga cgttccggcg acagatttca ggccgtcgtg cggaatgccg
    16801 ccttcatcaa ccggcagata ttcgtccgcc agaatctgca acttgtgatt gcgcacgtca
    16861 gactgctcgc cgtcaagatt gaaatagacg tgattagtca tattcaccgg gcaaggttta
    16921 tcaactgtgg cgcgataagt aatagagata cggttatcgt cggtcagacg atattgcacc
    16981 gtcgcgccga gattacccgg gaagccctga tcgccatcat ctgaactcag ggcaaacagc
    17041 acctgacgat cgttctggtt cacaatctgc cagcgacgtt tgtcgaaccc ttccgggccg
    17101 ccgtgcagct ggttaacgcc ctgacttggc gaaagcgtca cggtttcacc gtcaaaggta
    17161 taacggctat tggcgatgcg gttggcataa cgaccaatag aggcccccag aaacgcagcc
    17221 tgatcctgat agcattccgg gctggcacag ccgagcagcg cctcgcggac gctgccatcg
    17281 gaaagcggaa tacgggcgga aagtaaagtc gcaccccagt ccatcagcgt gactaccatc
    17341 cctgcgttgt tacgcaaagt taacagtcgg tacggctgac catcgggtgc cagtgcggga
    17401 gtttcgttca gcactgtcct gctccttgtg atggtttaca aacgtaaaaa gtctctttaa
    17461 tacctgtttt tgcttcatat tgttcagcga cagcttgctg tacggcaggc accagttctt
    17521 ccgggatcaa cgcgacgata cagccaccaa atccgccgcc ggtcatgcgt acgccacctt
    17581 tgtcaccaat cacagctttg acgatttcta ccagagtgtc aatttgcggc acggtgattt
    17641 cgaaatcatc gcgcatagag gcatgagact ccgccattaa ctcgcccata cgtttcaggt
    17701 cgccttgctc cagcgcgctg gcagcttcaa cggtgcgggc gttttcggtc aggatatgac
    17761 gcacgcgttt cgccacgatc gggtccagtt catgcgcaac agcgttgaac tcttcaatgg
    17821 tgacatcgcg cagagctggc tgctggaaga aacgcgcacc ggtttcgcac tgctcacggc
    17881 gagtattgta ctcactgcca accagggtac gtttgaagtt actgttgatg atgaccacag
    17941 ctacgccttt cggcatggaa actgctttgg tccccagtga gcggcagtca atcagcaagg
    18001 catgatcttt cttgccgagc gcggaaatta gctgatccat gatcccgcag ttacagccaa
    18061 caaactggtt ttctgcttcc tgaccattaa gcgcgatttg tgcgccgtcc agcggcagat
    18121 gataaagctg ctgcaatacg gttccgaccg cgacttccag tgaagcggaa gaacttaacc
    18181 cggccccctg cggcacattg ccgctgatca ccatgtccac accgccgaag ctgttgttac
    18241 gcagttgcag atgtttcacc acgccacgaa cgtagttcgc ccattgatag ttttcatgcg
    18301 cgacaatggg cgcatcgagg gaaaactcgt cgagctgatt ttcataatcg gctgccatta
    18361 cgcgaacttt acggtcatcg cgtggcgcac agctgatcac ggtttgataa tcaatcgcgc
    18421 agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc acgcggccag
    18481 gcgcctgaat ggtgtgagtg gcagggtagc caaatgcgtt ggcaaacaga gattgtgttt
    18541 tttctttcag actcatttct tacactccgg attcgcgaaa atggatatcg ctgactgcgc
    18601 gcaaacgctc tgctgcctgt tctgcggtca ggtctcgctg ggtctctgcc agcatttcat
    18661 aaccaaccat aaatttacgt acggtggcgg agcgcaacag aggcggataa aagtgcgcgt
    18721 gcagctgcca gtgttgattc tcttcgccat taaatggcgc gccgtgccag cccatagagt
    18781 aggggaagga gcactggaag aggttgtcat aacgactggt cagctttttc aacgccagtg
    18841 ctaaatcgct gcgctgggca tcggtcaaat cggtgatccg taaaacgtgg gctttgggca
    18901 gcagtagcgt ttcgaacggc caggcagccc agtaaggcac tacggctaac cagtgttcgg
    18961 tttcgacaac ggtacggcta ccgtctgcca gctcgcgctg aacataatcc accagcattg
    19021 gcgatttctg cccggcaaaa tattcttttt gcaggcggtc ttcgcgctca gcttcgttag
    19081 gcaggaagct atttgcccaa acctgtccgt gcggatgcgg gttagagcag cccatcgccg
    19141 cacctttgtt ttcaaagacc tgcacccacg ggtacgtttt tcccagctct gcggtttgct
    19201 cctgccaggt tttgacgatt tccgtcaatg ccgcaacgct cagttctggc aacgttttac
    19261 tgtgatccgg tgaaaagcag atcacccggc tggtaccgcg cgcgctctgg caacgcatca
    19321 aaggatcgtt gctttctggc gcatctggcg tgtcagacat caaggccgca aaatcattag
    19381 tgaaaacgta agtcccggtg taatcggggt ttttatcgcc tgtcacccgc acattacctg
    19441 cgcagaggaa gcaatctgga tcgtgcgcag gtaacacctg tttggctggc gtttcctgcg
    19501 ccccctgcca ggggcgctta gcgcggtgcg gtgaaaccag aatccattgc ccggtgagcg
    19561 ggttgtagcg gcgatgtgga tgatcaacgg gattaaattg cgtcatggtc gttccttaat
    19621 cgggatatcc ctgtggatgg cgtgactgcc agtgccaggt gtcctgcgcc atttcatcga
    19681 gtgtgcgcgt tacgcgccag ttcagttcgc gatcggcttt gctggcgtcc gcccagtagg
    19741 ccggaaggtc gccctcgcga cgcggtgcaa aatgatagtt aaccggtttg ccgcaggctt
    19801 tgctgaaggc attaaccacg tccagcacgc tgctgcctac gccagcgccg aggttgtaga
    19861 tgtgtacgcc tggcttgttc gccagttttt ccatcgccac gacgtgaccg tccgccagat
    19921 ccattacgtg gatgtaatcg cgtacgccag taccatcttc ggtcggataa tcgttaccaa
    19981 aaatcgccag cgagtcgcga cggcctacag caacctgggc gatgtatggc atcaggttat
    20041 tcggaatgcc ttgcggatct tcacccatat cgcccgacgg atgcgcgcca accgggttga
    20101 agtagcgcag cagggcaatg ctccagtccg gctgggcttt ttgcaggtcg gtgaggatct
    20161 gttccaccat caatttgctt ttgccataag ggctttgcgg tgtgccggtc gggaagcttt
    20221 caacgtatgg aattttgggc tgatcgccat aaacggtggc ggaggagcta aaaataaagt
    20281 ttttgacgtt agcggcgcgc atggcgctaa tcaggcgcag agtgccgttg acattgttgt
    20341 cgtaatattc cagcggtttt tgtaccgatt cgccaacggc tttcagcccg gcgaagtgga
    20401 tcacggtgtc gatagcgtga tcgtgcagga tctcggtcat caacgcttcg ttacgaatat
    20461 cgccttcaac aaacgttgga tgtttgccgc ctaaacgctc gataacaggc agtacgctgc
    20521 gcttactgtt acagaggtta tcaagaatga tgacatcatg accgttttgc agtaattgca
    20581 cacaggtatg acttccaatg taaccgctac caccggtaac cagaactctc ataattcgct
    20641 ccattaggct tatggtatga aataaccata gcataacaaa gatgcgaaaa gtgtgacatg
    20701 gaataaatta gtggaatcgt ttacacaaga atttagccgt tttttatgcg cgattaagtg
    20761 attataaaac agagggttta tgaatgattg cgctttttat ctgaaaaaag acgcggtttc
    20821 atgcctgcat gcgtcgaact gtaggccgga gagggggctt acgccacatc cggcacagtc
    20881 agcattacca actcagttta tttttgtcag cgcatagcga taaaggtcac cgtccggcac
    20941 gaactcaaga cgatgggtaa tacaggcagg cgcatcttca gcgtggtgcg aaacaaacaa
    21001 caattgcgtt tcaccttcgc taatcagcac atcaacaaaa cggcggataa gttggcgatt
    21061 gagcggatca agcccctgta gtggttcatc gagaataagc aatgtcggat gtttcaccag
    21121 cgcgcggacg atcagcgcca gacgctgctg tccccaggaa agactatgga acggaacgtc
    21181 agccgtgcgt ttatcaatgc cgagaatatc cagccactgc tgcaccagtt tttgctggcg
    21241 atccgaaacg gcctgataaa tgccaatcga atcaaaatag ccagaaagaa tcacattacg
    21301 cacggtagtg ctgacccggt aatccagatg caaactactg ctgacgtaac cgatatgctt
    21361 tttgatatcc cagatggttt cgccgctgcc gcgacgtcgg ccgaaaagcg tcaaatcgtt
    21421 gctgtaacct tgcggatgat cgccagtaat caggcttaat aacgtcgatt ttcccgcacc
    21481 atttggcccg acaatttgcc agtgctcgcc aggattcacc tgccagctaa ggttattaag
    21541 aatggggcga tcgttataag aaaccacgcc attattcagc acaatgcgcg gttcgttggt
    21601 gggtaaggcg tgacgtgctg aaggttcatc cggctccggc agttgcacac cttcaagctg
    21661 ttcgctatgc gccagttgcg cgacgagtgc ttgctggagc agttcctctt tagcgcccgt
    21721 ttccgctaac gtacaatccg ccagcacgcc agcaaactgg acaaattccg ggatctcatc
    21781 gaagcgattg agcaccagta ccagagtaat accggactga tgtaacgagg cgagtcgctc
    21841 agccagctgc tgacgtgagg caacatccag gccatcgaac ggctcatcaa gaatcaacaa
    21901 gtcaggctcc gacatcagcg cctgacacag cagggttttt cgcgtctcgc cagtggaaag
    21961 gtatttaaag cgtcggtcga ggagggcggt aataccgaac tgctgcgcca gttgcatgca
    22021 acgcggtgca tccttaactt catcctgaat gatctcagcc gtagtgcgtc cggtgtcatc
    22081 ttcgccaggg ccgagcatat cggtgttatt ccgctgccat tcgtcgctga cgagcttttg
    22141 caattgctcg aaggagagac gagtgatgtg ggaaaactgg ctttgccgtt cacctttcaa
    22201 aagcggaagt tcccccgcca gcgcacgggc cagcgccgac tttccacttc cattcgaacc
    22261 gacaaacgcc caactatcac ccgcgtttaa cgttagctga ggcaattgca gcgtttttgt
    22321 gtcgctaaga cgaaacgtgc cttgcaaaat ttgcaacgat gacattttat atcccatttt
    22381 gtgcagcgat tactgatagg gatacgtgtt tcataacaaa ttgtcaacac gcttagcata
    22441 gcgtggcgat aatcacgctg tcggcattaa agtaggccgt gacattctgt ccttgctgaa
    22501 gagaagtcgc ttcattaacc ggcactgtgg cgcacagtgt ttgcccgtcg ggtagcgcca
    22561 ttaatacttc gcactgctct gcgccgcgct caatatgact aataataccc ggtaattggt
    22621 tgtcggcgtt ttgtgcgacc gcttcgtcct gagtaatgcc tacccacggc gcttttagca
    22681 atatcaacac ttctttgcct tcatccagcc ccagacgtgc gccgctttgt gcggtaattg
    22741 cgactttcag gcgtgttttt ccgtcagcca gtaagacatc aacatgctgt tgaacgtcat
    22801 catgatcgcg ggcggtgatg gtgccgaacc actggttacg ggcgctggtt tgcagtgaaa
    22861 aacgtgagat cgcggccagc aggctgttca gcggcagggc gtcatcgtca cttaacacat
    22921 caaaggcttt ttgctggatt tgcgccagta agtcatagag ctgaatcagt cgctgaccat
    22981 agcgggtcag tactgcgccg ccgccacctt taccgcctgt tgcgcgctcg accagaatat
    23041 gctcacttaa ctgattcatc tcgttaatgg catcccaggc gcttttatag ctgataccgg
    23101 catctttcgc tccctggcta atggaaccgg aaagcgcaat gtgttttagt agcgaaatgc
    23161 ggcgcgggtc ggcgaataat ttttgttgga gcttaagggt gagaaggatt tcggcctgca
    23221 taacaatgtc ctggcaaaag tcttattgtg acggaaaacg aacgccacgc aaagctgacc
    23281 gcacaaaagg ggagtgcttt tctgtgctta gcggttagaa tagtctcatg actatatctg
    23341 gagttgacca tgttagagtt attaaaaagt ctggtattcg ccgtaatcat ggtacctgtc
    23401 gtgatggcca tcatcctggg tctgatttac ggtcttggtg aagtattcaa catcttttct
    23461 ggtgttggta aaaaagacca gcccggacaa aatcattgat tccctgaatg cccgcttagt
    23521 cgggcatttt ctttttctca acttcctgct tttcctgccg atattttttc ttatctacct
    23581 cacaaaggtt agtaataact gctggaaaat tccgagttag tcgttatatt gtcgcctaca
    23641 taacgttaca ttaaggggtt accaatggct cgtaaatggt tgaacttgtt tgccggggcg
    23701 gcactctctt tcgctgttgc tggcaatgca ctggcagatg aagggaaaat cacggtgttc
    23761 gccgccgcat cactgactaa cgcaatgcag gacattgcta cgcagtataa aaaagagaaa
    23821 ggcgtggatg tggtttcttc tttcgcttcg tcatctactc tcgcccgtca gattgaagcg
    23881 ggtgcgcctg cggatctgtt tatttctgcc gatcagaaat ggatggatta tgcagttgat
    23941 aaaaaagcga tcgatacagc tacgcgtcag acactgctcg gcaatagcct ggtcgttgta
    24001 gcaccgaaag ccagcgagca gaaagatttc atcatcgaca gcaaaactaa ctggacttcg
    24061 ctgctgaatg gcggtcgcct cgcggttggc gatccggaac atgtccctgc tggcatttat
    24121 gcaaaagaag cactgcaaaa actgggcgca tgggatacgc tctccccgaa actggctccg
    24181 gcggaagatg ttcgtggcgc gctggcgttg gtcgaacgca atgaagcgcc gttgggcatt
    24241 gtttacggtt ctgacgcagt tgccagcaaa ggggtaaaag tggttgccac cttcccggaa
    24301 gattcacata aaaaagtgga atatccggtt gctgttgtgg aagggcataa caatgcgaca
    24361 gtgaaagcat tttatgatta tctgaaggga ccgcaggctg cggaaatctt taaacgttac
    24421 ggatttacaa ccaagtaatg atactgaccg atccagaatg gcaggcagtt ttattaagcc
    24481 tgaaagtttc ttccctggct gtgctgttta gcctgccgtt tgggatcttt tttgcctggt
    24541 tactggtgcg ttgcacgttt ccgggcaaag ctttgctcga cagcatactg catctaccgc
    24601 tggtgttacc gcccgtggtc gtcggttact tattattagt ttcgatggga cggcgcggat
    24661 ttatcggtga acgtctgtat gactggtttg gtattacctt cgcctttagc tggcgcggcg
    24721 cggttctcgc tgccgccgtc atgtcgtttc cgctaatggt gcgggcaatt cgtctggcgc
    24781 tggaaggggt tgatgtcaaa ctggaacagg ccgcaagaac actgggggcc gggcgctggc
    24841 gcgttttctt tactatcacg ttaccgctga ccttaccggg aattattgtt ggtacggtac
    24901 tggcttttgc tcgttctctc ggtgaatttg gcgcaaccat cacctttgtg tcgaacattc
    24961 ctggtgaaac gcgaaccatt ccttctgcca tgtataccct gattcagacc cccggcgggg
    25021 aaagtggcgc agcgagactg tgcattattt ctattgcgct ggcgatgatc tccctgttga
    25081 tttcagaatg gctggccaga atcagccgtg aacgggcggg gcgctaatca tgctggaact
    25141 gaatttttcc cagaccttgg gcaaccattg cctgactatt aatgaaacgc tgcccgccaa
    25201 tggcatcact gctatctttg gcgtctccgg tgccggaaaa acttcgctga ttaacgccat
    25261 cagtggactg acgcgcccgc aaaaagggag gattgtcctc aatggtcggg tactaaatga
    25321 tgccgaaaaa ggtatctgcc tgacgccaga aaaacgtcgc gttggctatg tttttcagga
    25381 tgcacggctg ttcccgcatt acaaagtgcg tggcaatttg cgctacggca tggcgaaaag
    25441 catggtcgat cagttcgata agctggtggc gcttttaggc attgaaccgt tgcttgaccg
    25501 tttaccgggc agcctgtccg gaggcgaaaa acagcgcgtg gcgattggtc gggctttgct
    25561 gacggctccg gaattgctgt tgctggatga accgctggcg tcactggata ttccgcgtaa
    25621 acgcgaactg ttgccttatc tgcaacgtct gacgcgggaa atcaacattc cgatgttgta
    25681 tgtcagccat tcgctggatg agatcctcca tctggcagac agagtgatgg tactggaaaa
    25741 cggtcaggtg aaagcctttg gcgcgctgga agaagtctgg ggtagtagcg tgatgaatcc
    25801 gtggctgccg aaagagcaac aaagtagcat tctgaaagtg acggtacttg agcatcatcc
    25861 gcattacgcg atgactgcgc tggcgctggg cgatcagcat ttgtgggtca ataagctgga
    25921 cgaaccgctg caagctgcgc tacgtatccg cattcaggct tccgatgttt ctctggtttt
    25981 acaaccgccg cagcaaacca gcattcgtaa cgtattgcgg gcaaaagttg ttaatagtta
    26041 tgacgacaac ggccaggtgg aagtggagct ggaagtcggc ggtaaaacgc tgtgggcgcg
    26101 tatcagcccg tgggccaggg atgaactggc gatcaaacct ggcctgtggc tgtacgcgca
    26161 aattaaaagt gtgtcgataa ccgcctgatt aaatcaggtg gctataaatg aactgggcaa
    26221 tgctgtcggt ggtgttatca ccaatcacaa tgttggcgcg cgcttttacc gcgtcatcgg
    26281 cgttgcccat cgccacgcct gtaccagcgg cttccagcat actgatatca ttaaagttat
    26341 cgccgaatgc cacgacgttt tccatcgacc aaccttgcgc ctcaacccat ttcgtcaaac
    26401 gtttaccttt gctgttgccg ccgcgtgcaa tatcaacctg atcgtgccag gaccattcac
    26461 actccagtcc cagttcatgt tcgacatgct taccaaaatg ctgcaattgc ggcaggtcat
    26521 cgtgcgtcag ggcgaacttc catacggcgt taacttgttg cgccgtttca gccagagaag
    26581 cgacttgtgt gaaagtcgga cgctgttccg gcggcagggt ttgcgcccag ttagacgtgc
    26641 gaatgacatg cccggtcggg tgctcataga ccattgcatc atcgacatac atcagaccgt
    26701 gaatgtggtg ttcattcagc atctcaatga gttgcagggc tttattaacg ggcattgggt
    26761 ccgcttccag cacggttttt gcatgataat catacaaata ggtgccatta cagcaaatag
    26821 caggtgtatc cagcgccagc gcctgataaa aaggatgaat agcgacgtga tggcgacctg
    26881 tgacgatgat taattgatag cctgcttcgc gagcgcgggc cagggcttct atcgatgaag
    26941 gaagcagggt ctttttcggg gtcaataagg tgccgtctaa gtcgagagca atcacgcgtg
    27001 tggtcatggc gtattccaga ttaaggttaa gaattttcgt ctgcgcgaat ggtacaccga
    27061 taccactatc ggacaaaatt ctgcgtttta attcagcatt caccgccaaa agcgactaat
    27121 tttagctgtt acagtcagtt gctaaatgca aaggagcatt catgaagcaa acagtttata
    27181 tcgccagccc tgagagccag caaattcacg tctggaacct gaatcatgaa ggcgcactga
    27241 ctctgacaca ggttgtcgat gtgccggggc aggtgcagcc gatggtggtc agcccggaca
    27301 aacgttatct ctatgttggt gttcgccctg agtttcgcgt cctggcgtat cgtattgccc
    27361 cggacgatgg cgcactgacc tttgccgcag agtctgcgct gccgggtagt ccgacgcata
    27421 tttccaccga tcaccagggg cagtttgtct ttgtaggttc ttacaatgcg ggtaacgtga
    27481 gcgtaacgcg tctggaagat ggcctgccag tgggcgtcgt cgatgtggtc gaggggctgg
    27541 acggttgcca ttccgccaat atctcaccgg acaaccgtac gctgtgggtt ccggcattaa
    27601 agcaggatcg catttgcctg tttacggtca gcgatgatgg tcatctcgtg gcgcaggacc
    27661 ctgcggaagt gaccaccgtt gaaggggccg gcccgcgtca tatggtattc catccaaacg
    27721 aacaatatgc gtattgcgtc aatgagttaa acagctcagt ggatgtctgg gaactgaaag
    27781 atccgcacgg taatatcgaa tgtgtccaga cgctggatat gatgccggaa aacttctccg
    27841 acacccgttg ggcggctgat attcatatca ccccggatgg tcgccattta tacgcctgcg
    27901 accgtaccgc cagcctgatt accgttttca gcgtttcgga agatggcagc gtgttgagta
    27961 aagaaggctt ccagccaacg gaaacccagc cgcgcggctt caatgttgat cacagcggca
    28021 agtatctgat tgccgccggg caaaaatctc accacatctc ggtatacgaa attgttggcg
    28081 agcagggact actgcatgaa aaaggccgtt atgcggtcgg gcagggacca atgtgggtgg
    28141 tggttaacgc acactaaccg ctgatttaac cggcgcagtc tctcctgcgc cggtgtatta
    28201 acttatctcc tgtaacgcat gtctctggcg ttcgacgata ttggtccaca aattgtcttt
    28261 atcgtcagtc cacaaattaa tcagcaaggc aaaaaagcgt tcagctgccg gagaaagcac
    28321 ggcatctttg cggcgaataa tccccaatgt ccgacgtata acgggttcca ccagtgggat
    28381 accaataatc gaagaatagg gcgcatgggg cattgccaga ccaggaagcg ccgaaatccc
    28441 cagtcctgcc tccactagtc ctaatgacgt cgaaagatga cgcacttcgt aaaaccagtc
    28501 cagcttccag ggcttgtcgg ctagctgttg ttctatcaac aatcggttgc cgctggagga
    28561 acgtacgcca atcattttgt aacccactaa ctcctgccat tctacaagct gctttttggc
    28621 caatggatgg tcacgccgac aggccagcac gaatggctcg ttgaccagtg gggtaaaatc
    28681 aatggatgaa tttgtgacgt tgttcatgtt tatgccaaag tctgattcgt tacacagcac
    28741 cgattccatg caattattcg tgccttgttc cagaatccgc actttaatat tgggatacag
    28801 ctcattaaat ttaccgattg ccagcggtaa aaaataaaat actgcggtcg gaatacacgc
    28861 taacgtcacc ataccacgat gataagcatt catatcacga atattaaaaa gcgtttcatc
    28921 gaatttcttt attaattccc gcgcctccgg gagcaacctt ttccctgctt ttgttaacgt
    28981 aaccttgcga gttgtacgct caaatagttg aatatgtaaa tcttcctcca tcttttttat
    29041 tcttcgcgtt aatgcaggtt gcgtaatatt gagtgattta gcggcattgt taaatgaact
    29101 ggactctgcc agtatgacaa atgccttcat acttgataat tcatgcttca ttattactcc
    29161 ggaaaatgga agcgacgatt ttgggtggct ggccgttaaa aattttaact gcatttagcc
    29221 aacttaaatt aatgaaaaaa tgttattaat cgttgagcta aagtcattag agatgctttg
    29281 cccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa
    29341 aaaataccct gcgtgatgat gcgaggtgga acctcgaggg gcgcgttctt gttagcggaa
    29401 catttacccg aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt
    29461 aacgatctgg aaattgacgg tattggcggc ggtaatccgc tgaccagtaa agtcgccatt
    29521 attagccgtt ccagcgatcc gcgtgctgat gtcgattatc tgtttgctca agttattgtc
    29581 catgagcaac gtgtcgatac cacgcctaat tgtggcaata tgctgtctgg tgttggggca
    29641 ttcgccattg aaaatggttt gattgcagcg acttcaccag ttacccgcgt acgtatccgc
    29701 aacgtcaata ctggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag
    29761 tacgagggta gcgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact
    29821 ttcctgaatg ccgccggaac caaaaccgga aaagtcttcc cgactgataa tcagattgat
    29881 tattttgacg atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg
    29941 gctgaatatc tgggaaaaac aggttatgaa ttaccggcgg aactggatgc cgacaaagca
    30001 ttattagcac gcattgaatc tatccgtcta caagcgggta aagcaatggg cttaggtgat
    30061 gtcagtaata tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca
    30121 attaatgtac gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct
    30181 attgctattt ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct
    30241 gtaggatacg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt
    30301 aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacgac cagaaaaata
    30361 ttttccggtg aagtttatct tccctgaaaa aattcgttgt caggataagg acaatcaata
    30421 aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta
    30481 tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca
    30541 ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtgggtctg
    30601 gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctcaatggtg
    30661 gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct
    30721 tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc
    30781 ggtttaggta aacgtattgc ctatctgctg attggtaaaa tcggtaacac cacgctgggt
    30841 ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc
    30901 gcgcgtgcgg gcggcattgt gttaccgatc atcaacagcg tggcggtggc tttggggtcc
    30961 gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc
    31021 accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa
    31081 atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcgct ggccgccgga
    31141 ttgccgggca tcattatgct gctggtcacc ccgctggtaa tttacaccat gtatccacca
    31201 gaaattaaaa aggtggataa caaaaccatc gctaaagcgg gccttgccga actgggaccg
    31261 atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc tggcgctact gggctggatt
    31321 ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg
    31381 ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc
    31441 ttaatctggt acggcggtat tatcggatta agctccttat tatcgaaagt taaattcttc
    31501 gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct
    31561 ttcttcgtta ttattttcct cagcattatc gtgcgttatt tcttcgcttc cggtagtgcc
    31621 tatatcgttg ctatgttacc ggtatttgcc atgctggcga acgtctccgg cgcaccgtta
    31681 atgttaaccg cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat
    31741 ggcggcgcgg caggtccggt catctttggc gtgggttata acgatattaa atcctggtgg
    31801 ttggtcggtg cggtactgac gatattaacc ttcctggtgc atatcaccct cggcgtgtgg
    31861 tggtggaata tgctgatcgg ctggaacatg ctgtaaatat tctcgtcata cttcaagttg
    31921 catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggg
//
LOCUS       DAZFYC010000048        31722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFYC010000048 DAZFYC010000000
VERSION     DAZFYC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.048"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(210..1583)
                     /gene="argH"
                     /locus_tag="ACHKW5_002469"
     CDS             complement(210..1583)
                     /gene="argH"
                     /locus_tag="ACHKW5_002469"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO7511843.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1644..2420)
                     /gene="argB"
                     /locus_tag="ACHKW5_002470"
     CDS             complement(1644..2420)
                     /gene="argB"
                     /locus_tag="ACHKW5_002470"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO7511844.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2428..3432)
                     /gene="argC"
                     /locus_tag="ACHKW5_002471"
     CDS             complement(2428..3432)
                     /gene="argC"
                     /locus_tag="ACHKW5_002471"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO7511845.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3586..4737
                     /gene="argE"
                     /locus_tag="ACHKW5_002472"
     CDS             3586..4737
                     /gene="argE"
                     /locus_tag="ACHKW5_002472"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO7511846.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5089..7740
                     /gene="ppc"
                     /locus_tag="ACHKW5_002473"
     CDS             5089..7740
                     /gene="ppc"
                     /locus_tag="ACHKW5_002473"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO7511847.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7923..9656
                     /locus_tag="ACHKW5_002474"
     CDS             7923..9656
                     /locus_tag="ACHKW5_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7511848.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPVLDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9805..10656
                     /locus_tag="ACHKW5_002475"
     CDS             9805..10656
                     /locus_tag="ACHKW5_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7511849.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10643..10984)
                     /locus_tag="ACHKW5_002476"
     CDS             complement(10643..10984)
                     /locus_tag="ACHKW5_002476"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7511850.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10986..11864)
                     /locus_tag="ACHKW5_002477"
     CDS             complement(10986..11864)
                     /locus_tag="ACHKW5_002477"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO7511851.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(11830..14127)
                     /locus_tag="ACHKW5_002478"
     CDS             complement(11830..14127)
                     /locus_tag="ACHKW5_002478"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7511852.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14177..14497)
                     /locus_tag="ACHKW5_002479"
     CDS             complement(14177..14497)
                     /locus_tag="ACHKW5_002479"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7511853.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14512..15591)
                     /locus_tag="ACHKW5_002480"
     CDS             complement(14512..15591)
                     /locus_tag="ACHKW5_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO7511854.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15900..18401
                     /gene="ptsP"
                     /locus_tag="ACHKW5_002481"
     CDS             15900..18401
                     /gene="ptsP"
                     /locus_tag="ACHKW5_002481"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7511855.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18413..19075
                     /gene="fsa"
                     /locus_tag="ACHKW5_002482"
     CDS             18413..19075
                     /gene="fsa"
                     /locus_tag="ACHKW5_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7511856.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGIVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19086..20189
                     /gene="gldA"
                     /locus_tag="ACHKW5_002483"
     CDS             19086..20189
                     /gene="gldA"
                     /locus_tag="ACHKW5_002483"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO7511857.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20464..21081
                     /locus_tag="ACHKW5_002484"
     CDS             20464..21081
                     /locus_tag="ACHKW5_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO7511858.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21108..22013)
                     /gene="yijE"
                     /locus_tag="ACHKW5_002485"
     CDS             complement(21108..22013)
                     /gene="yijE"
                     /locus_tag="ACHKW5_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO7511859.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22107..24287)
                     /gene="katG"
                     /locus_tag="ACHKW5_002486"
     CDS             complement(22107..24287)
                     /gene="katG"
                     /locus_tag="ACHKW5_002486"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO7511860.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24616..25506)
                     /gene="metF"
                     /locus_tag="ACHKW5_002487"
     CDS             complement(24616..25506)
                     /gene="metF"
                     /locus_tag="ACHKW5_002487"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO7511861.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25582..25716
                     /locus_tag="ACHKW5_002488"
     CDS             25582..25716
                     /locus_tag="ACHKW5_002488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511862.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25855..28287)
                     /gene="metL"
                     /locus_tag="ACHKW5_002489"
     CDS             complement(25855..28287)
                     /gene="metL"
                     /locus_tag="ACHKW5_002489"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO7511863.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28290..29450)
                     /gene="metB"
                     /locus_tag="ACHKW5_002490"
     CDS             complement(28290..29450)
                     /gene="metB"
                     /locus_tag="ACHKW5_002490"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO7511864.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGYGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29727..30044
                     /gene="metJ"
                     /locus_tag="ACHKW5_002491"
     CDS             29727..30044
                     /gene="metJ"
                     /locus_tag="ACHKW5_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO7511865.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30228..30836
                     /locus_tag="ACHKW5_002492"
     CDS             30228..30836
                     /locus_tag="ACHKW5_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO7511866.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30981..31722
                     /locus_tag="ACHKW5_002493"
                     /pseudo
     CDS             30981..31722
                     /locus_tag="ACHKW5_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7645 a         8833 c         8067 g         7177 t
ORIGIN      
        1 tgattttcat ggggagtgtg aaggtaaaga agacacaatg ataaaaagga atgtgacaaa
       61 taatggttct ggttgggcgt taaatttaag ctcaccagaa tgctgacaaa tttctgcctg
      121 atgtggcgta aacgccttat ccgcctacga agtgtcgcac tttgtaggcg tggtaagctc
      181 cccattttca ggggagcaat ataaacaaat tactctaacc gcgcctgcgc aaaagcaatt
      241 gcctgcgcca cctgctgcgg tgagacgccg ccttttgccg cacgcttgtc gaggcacgat
      301 tgcagcgaca gaatcggata gacatcttcg tcaatcaccg ggctgaattt ctgcaactcg
      361 tcgagcggca gatcttccag cggtttgccc tgacgaatgg cttccaccac cgcttcacca
      421 acaatatggt gcgcctcgcg gaacggtacg cctttcgcca ccagataatc cgccagttcg
      481 gtggcgttgg cgtaaccctg ctgagccgct tcctggcaac gtggacgttt cacctgaatg
      541 ccgtccagca ccagcgccgc catatgcagg cagtccagcc aggtatcgag cgcgtcgaac
      601 agaccttctt tgtcttcctg catatctttg ttgtaagcca gcggcaaacc tttcagcgtc
      661 atcatcatgc cggttaacgc cccctgcacc cgaccgcatt taccgcgaat cagctccagc
      721 gcatccgggt ttttcttctg cggcattaat gatgaaccgg aagtcacgcg gtcagaaagc
      781 tccacaaacc ccgcttcgcc ggtgttaaag aaaatcagat cttcagcaaa acgcgacaga
      841 tgcaccatgc cgatagcggc agcagaaagc agttccaaca catggtcacg gtcagaaacg
      901 ctgtcgagac tgttacgggt cgccgaagca aagcccagcc agcctgctaa ctgttcacgg
      961 tcgatttcat aggccgttcc cgccagcgcg ccacagccta gcgggctgac atccagacgc
     1021 ttaagcgcat cctgcaaacg gctttcatca cgcgccagca tctcaacata ggccaggcac
     1081 cagtgcgcga acgtcaccgg ctgggcgcgt tgcaggtgag tgtaacctgg cattaccgcg
     1141 tcctgattgt tttgtgcggt ttccaccagc gccgattgca gctgccggtt agccgtcagt
     1201 aactcgctaa cggtatcttt gcaccacagt ttcaggtcag tcgctacctg atcattacgg
     1261 ctacgcccgg tatgcagctt tttgcctaac tggcccactt tgtcgatcag tttgccttcc
     1321 acccagctat ggatatcttc ggcgtcgctt tcaaggattt gttgtggcct ggcgcgaaca
     1381 tcttccagca acacgttcag cgcctcttcc agttgcgcct gctcttctgc ggttaacacg
     1441 cctaccgtga ccagggcttt ggaccaggcc acagagccaa caatatcctg ctccgccaga
     1501 cggtaatcaa agcgcagtga gtcgttgaat tgtttgaacc gttgatctgc tgcctgggta
     1561 aaacgcccgc cccaaagtgc cataactctg tttccttatt ttgaaattca atgccggaaa
     1621 gctgcgcctc cggccaacaa aacttaagct aaaatccgcg tacccatcgg cataccgtta
     1681 aacagtgccg gaagctgctc cgcatgacgc caggaggcga tatctaccgg acggcccagc
     1741 gtgcgggccg catccagcgc cgcgttcact ttcactatca tgccgtcagt aataatgccc
     1801 tgctcaatca gttgttctgc tttcgcggcg gtcatttcgg caatgcgttg ccctttgccg
     1861 tcgagaatgc cgctgacgtc ggagagcaaa atcagatccg cgcccagcgt tgccgccagc
     1921 gccgttgccg cctggtcggc attgacgttc atcagttgcc cttcgtctgt tacgccaatg
     1981 gagctgacca ccggcagata accgttctcc agcaaggagt tgataagctt aggcgaacct
     2041 ggctgcgcca gtccaacatg acctaactct tcatcaagct gggtcacttt gacgctgtcg
     2101 ccgtcaccga gaaacaaacc tacggccgca atctgatgtt tcttcgccca tgccaacagg
     2161 gttttatttg ccgttcccgc cagtgctccg gtgataatgt ctatctgatc agcaggcgtc
     2221 acccgcaggc cgtttttctt tttcaccggc agattcagcc ctttcatcag ctcatccacc
     2281 acgcaaccgc cgccgtgcac aatcaccagc ggacgctgat gtgactcacg ataattcacc
     2341 agtgcgctaa acagacgttc cagcgcctct tcactatcca gcagtacgcc gcccagtttg
     2401 ataattaatg gattcatcat tgcaccctta aataagagac tgcgtttccg catagccgaa
     2461 acgaatattg gcgcactgta ccgcttgtgc cgccgcgcct ttcagtaagt tgtcttcggt
     2521 cgccacaata atcagatgct cgccctgaac ggcaaacccg atatcgcaaa atggcagccc
     2581 aacgacattt ttcagcgccg gaacgccttt gtcatacagc cgcaccagcg gtttatgggc
     2641 atacgcctgc tgtaacactt gcgcgacttg cgcctgggtc acacccgatt tcaggcggca
     2701 ggtaatggtt tcgagaatgc cgcgcgggaa attgcccaga tgtggggtga agataacgtc
     2761 agcaccgagg tgtgtggcga tctctggttg atggcgatga gtaaagacgc catacggttg
     2821 caggctaact tcacaaaagc tgtttgaaat ggccgcttta cgccctgcac cgctcacgcc
     2881 gctggtggcg ttgatcaccg gccactgatt gaggtcaaga agatcggcat caatcaacgg
     2941 tttcagcgcc agctgtgccg ccgtcggata acagcccggc accgcaatca aattcgcttc
     3001 ttttaattta ttaccgcacc actccgccag accgtaggct gcctgttcca acagttccgg
     3061 gtattgatgg gtaaagccgt aatatttttc atagaaggtg gcgtcgttaa cacgaaacgc
     3121 gccggaaagg tcgaacacca cgcagcccgc ttcaagaaac tgcggcgcta aatcgtggct
     3181 aacttcatgg gcggtggcga gaaacactac gtccacccct gggctaaact cgctgatatc
     3241 cgacatcggc tgcaacggca gatcaacgat gccttttagc tgcggatgca aatcggagat
     3301 taacttgccc gcatcattgc tttgcgctga aacagtcaaa gcggttatgt tcatatgcgg
     3361 atggcgattt acataggtca ctagctctgc gccagcgtag ccgctggcac ccacaatcag
     3421 cgtattcaac atcggggcta ttcaccttct tatgtctggt tgccaggtta aacgtaaaac
     3481 attcacctta cggctggtgg gttttattac gctcaacgtt agtgtatttt tattcataaa
     3541 tactgcatga atattgatac tatcatgacc agaggtgtgt caacaatgaa aaacaaatta
     3601 ccgccattta tcgagattta ccgtgctctg attgccacac cttcaataag cgccacggaa
     3661 gaggcactcg atcaaagcaa tgcagattta atcactctgc tggcggactg gtttaaagat
     3721 ttaggcttca atgtggaagt gcagcctgtg ccaggaactc gcaacaaatt taatatgctg
     3781 gcaagtatcg gacagggggc tggcggcttg ttgctggcgg ggcataccga tacggtacca
     3841 tttgatgacg gtcgctggac gcgtgatccg tttacactga cggagcatga cggcaagctt
     3901 tacggcttag gcaccgccga catgaaaggc ttttttgcgt ttatccttga tgcgctacgc
     3961 gatgtcgacg tcacgaaact gaaaaaaccg ctctacattc tggcgactgc tgatgaagaa
     4021 accagtatgg ccggagcgcg ttattttgcc gaaactaccg ccctgcgccc ggattgcgcc
     4081 atcattggcg aaccgacgtc actacaaccg gtacgcgcac ataaaggtca tatctctaac
     4141 gccatccgta ttcagggcca gtcggggcac tccagcgatc cagcacgcgg agttaacgct
     4201 atcgaactaa tgcacgacgc catcgggcat attttgcaat tgcgcgataa cctgaaagaa
     4261 cgttatcact acgaagcgtt taccgtgcca taccctacgc tcaacctcgg gcatattcac
     4321 ggtggcgacg cttctaaccg tatttgcgct tgctgtgagt tgcatatgga tattcgtccg
     4381 ctgcctggca tgacactcaa tgaacttaat ggtttgctca acgatgcatt ggctccggtg
     4441 agcgaacgct ggccgggtcg tctgacggtc gacgagctgc atccgccgat ccctggctat
     4501 gaatgcccac cgaatcatca actggttgaa gtagttgaga aattgctcgg agcaaagacc
     4561 gaagtggtga actactgtac cgaagcgccg tttattcaaa cgttatgccc gacgctggtg
     4621 ttggggcctg gctcaattaa tcaggctcat caacctgatg aatatctgga aacacggttt
     4681 atcaagccca cccgcgaact gataacccag gtaattcacc atttttgctg gcattaaaac
     4741 gtaggccgga taaggcgctc gcgccgcatc cggcactgtt gtcaaactcc agtgccgcaa
     4801 taatgtcgga tgcgatactt gcgcatctta tccgacctac acctttggtg ttacttgggg
     4861 cgatttttta acatttccat aagttacgct tatttaaagc gtcgtgaatt taatgacgta
     4921 aattcctgct atttattcgt ttgctgaagc gatttcgcag catttgacgt caccgctttt
     4981 acgtggcttt ataaaagacg acgaaaagca aagcccgagc atattcgcgc caatgcgacg
     5041 tgaaggatac agggctatca aacgataaga tggggtgtct ggggtaatat gaacgaacaa
     5101 tattccgcat tgcgtagtaa tgtcagtatg ctcggcaaag tgctgggaga aaccatcaag
     5161 gatgcgttgg gagaacacat tcttgaacgc gtagaaacta tccgtaagtt gtccaaatct
     5221 tcacgcgctg gcaatgatgc taaccgccag gagttactca ccaccttaca aaatttgtcg
     5281 aacgacgagc tgctgcccgt tgcgcgtgcg tttagtcagt tcctgaacct ggccaacacc
     5341 gccgagcaat accacagcat ttcgccgaaa ggcgaagctg ccagcaaccc ggaagtgatc
     5401 gcccgcaccc tgcgtaaact gaaaaaccag ccggaactga gcgaagacac catcaaaaaa
     5461 gcagtggaat cgctgtcgct ggaactggtc ctcacggctc accctaccga aattacccgt
     5521 cgtacactga tccacaaaat ggtggaagtg aacgcctgct taaaacagct cgataacaaa
     5581 gatatcgctg actacgaaca caaccagctg atgcgtcgcc tgcgccagtt aatcgcccag
     5641 tcatggcata ccgatgaaat ccgtaagctg cgtccaagcc cggtagatga agccaaatgg
     5701 ggctttgccg tagtggaaaa cagcctgtgg caaggcgtac caaattacct gcgcgaactg
     5761 aacgaacaac tggaagagaa cctcggctac aaactgcccg tcgaatttgt tccggtccgt
     5821 tttacttcgt ggatgggcgg cgaccgcgac ggcaacccga acgtcactgc cgatatcacc
     5881 cgccacgtcc tgctactcag ccgctggaaa gccaccgatt tgttcctgaa agatattcag
     5941 gtgctggttt ctgaactgtc gatggttgaa gcgacccctg aactgctggc gctggttggc
     6001 gaagaaggtg ccgcagaacc gtatcgctat ctgatgaaaa acctgcgttc tcgcctgatg
     6061 gcgacacagg catggctgga agcgcgcctg aaaggcgaag aactgccaaa accagaaggg
     6121 ctgctgacac aaaacgaaga actgtgggaa ccgctctacg cttgctacca gtcacttcag
     6181 gcgtgtggca tgggtattat cgccaacggc gatctgctcg acaccctgcg ccgcgtgaaa
     6241 tgtttcggcg taccgctggt ccgtattgat atccgtcagg agagcacccg ccataccgaa
     6301 gcgctgggcg agctgacccg ctacctcggt atcggcgact acgaaagctg gtcagaggcc
     6361 gacaaacagg cgttcctgat ccgcgaactg aactccaaac gtccgcttct gccgcgcaac
     6421 tggcaaccaa gcgccgaaac gcgcgaagtg ctcgatacct gccaggtgat tgccgaagcg
     6481 ccgcaaggct ccattgccgc ctacgtgatc tcgatggcga aaacgccgtc cgacgtactg
     6541 gctgtccacc tgctgctgaa agaagcgggt atcgggtttg cgatgccggt tgctccgctg
     6601 tttgaaaccc tcgatgatct gaacaacgcc aacgatgtca tgacccagct gctcaatatt
     6661 gactggtatc gtggcctgat tcagggcaaa cagatggtga tgattggcta ttccgactca
     6721 gcaaaagatg cgggagtgat ggcagcttcc tgggcgcaat atcaggcaca ggatgcatta
     6781 atcaaaacct gcgaaaaagc gggtattgag ctgacgttgt tccacggtcg cggcggttcc
     6841 attggtcgcg gcggcgcacc tgctcatgcg gcgctgctgt cacaaccgcc aggaagcctg
     6901 aaaggtggcc tgcgcgtgac tgaacagggc gagatgatcc gctttaaata tggtctgcca
     6961 gaaatcaccg tcagcagcct gtcgctgtac accggggcga ttctggaagc caacctgctg
     7021 ccaccacctg agccgaaaga gagctggcgt cgcattatgg atgaactgtc agtcatctcc
     7081 tgcgatctct accgtggcta cgtacgtgaa aacaaagatt ttgtgcctta cttccgctcc
     7141 gctacgccgg aacaagaatt gggcaaactg ccgttgggtt cacgtccggc gaaacgtcgc
     7201 ccaaccggcg gcgtcgagtc gctgcgcgcc ataccgtgga tttttgcctg gacgcaaaac
     7261 cgcctgatgc tccccgcctg gctgggtgca ggtacggcgc tgcaaaaagt ggtcgaagat
     7321 ggcaaacaga gcgagctgga agccatgtgc cgcgattggc cattcttctc gacgcgtctc
     7381 ggcatgctgg agatggtctt cgccaaagca gacctgtggc tggcggaata ctatgaccaa
     7441 cgcctggtag acaaagcact gtggccgtta ggtaaagagt tacgcaacct gcaagaagaa
     7501 gacatcaaag tggtgctggc gattgccaac gattcccatc taatggccga tctgccgtgg
     7561 attgcagagt ctattcagct acggaatatt tacaccgacc cgctgaacgt attgcaggcc
     7621 gagttactgc accgctcccg ccaggcagaa aaagaaggcc aggaaccgga tcctcgcgtc
     7681 gaacaagcgt taatggtcac tattgccggg attgcggcag gtatgcgtaa taccggctaa
     7741 tcttcctctt ctgcaaaccc tcgtgctttt gcgcgagggt tttctgaaat acttctgttc
     7801 taacaccctc gttttcaata tatttctgtc tgcattttat tcaaattctg aatatacctt
     7861 cagatatcct taaggaattg tcgttacatt cggcgatatt tttttcaaga caggttctta
     7921 ctatgcattc cacagaagtc caggctaaac ccctttttag ctggaaagcc ctgggttggg
     7981 cactgctcta cttttggttt ttctctactc tgctacaggc cattatttac atcagtggtt
     8041 atagtggcac caacggcatt cgcgactcgc tgttattcag ctcgctgtgg ttgatcccgg
     8101 tattcctctt tccgaagcgg attaaaatta ttgccgcagt aatcggcgtg gtgctatggg
     8161 cggcctctct ggcggcgcta tgctactacg tcatctacgg gcaggagttc tcgcagagcg
     8221 ttctgtttgt gatgttcgaa accaacacca acgaagccag cgagtattta agccagtatt
     8281 tcagcctgaa aattgtgctt atcgcgctgg cctatacggc ggtggcagtt ctgctgtgga
     8341 cacgcctgcg cccggtctat attccaaagc cgtggcgtta tgttgtctct tttgccctgc
     8401 tttatggctt gattctgcat ccgatcgcca tgaatacgtt tatcaaaaac aagccgtttg
     8461 agaaaacgtt ggataacctg gcctcgcgta tggagcctgc cgcaccgtgg caattcctga
     8521 ccggctatta tcagtatcgt cagcaactaa actcgctaac aaagttactg aatgaaaata
     8581 atgccttgcc gccactggct aatttcaaag atgaatcggg taacgaaccg cgcactttag
     8641 tgctggtgat tggcgagtcg acccagcgcg gacgcatgag tctgtacggt tatccgcgtg
     8701 aaaccacgcc ggtgctggat gcgctgcata aaaccgatcc gaatctgacc gtgtttaata
     8761 acgtagttac gtctcgtccg tacaccattg aaatcctgca acaggcgctg acctttgcca
     8821 atgaaaagaa cccggatctg tatctgacgc agccgtcgct gatgaacatg atgaaacagg
     8881 caggttataa aaccttctgg atcaccaacc agcagacgat gaccgcccgc aataccatgc
     8941 tgacggtatt ttcgaagcag accgacaaac agttctatat gaaccagcaa cgtacgcaga
     9001 gtgcgcgtga atacgacacc aacgtgctga aaccattcca ggaagtgctg aaggacccgg
     9061 cgccgaagaa actgatcatc gttcatctgc tgggtacgca tatcaaatac aaataccgct
     9121 acccggaaaa tcagggcaag tttgatggca ataccgatca tgttccgcct ggattaaacg
     9181 cggaagagct ggagtcatat aacgattatg acaacgctaa cctgtataac gatcatgtgg
     9241 ttgccagcct gattaaagac tttaaagcag caaacccgaa cggtttcctg gtttatttct
     9301 ctgaccacgg tgaagaggtt tacgacacgc cgccgcacaa aactcagggg cgtaacgaag
     9361 acaacccgac gcgccacatg tacaccattc cgttcctgct gtggacgtcg gaaaaatggc
     9421 aagcgactca tccgcgtgat ttctcgcagg atgttgatcg taaatacagc ctggcggaac
     9481 tgatccacac ctggtcagat ttagcaggct tatcttacga cggttacgat ccaacccgtt
     9541 cagtggtgaa tccgcagttc aaagaaacta cccgctggat tggtaacccg tacaagaaaa
     9601 acgcgctgat cgattacgac acgctgccgt atggcgatca ggtgggtaat cagtaaatga
     9661 cctttgtatc gcgtctggcg ctatttgccg gatgcgatac tgccgcaatg tttacatcga
     9721 actcccctca ggccacactt ttcaggtggc cttttttaat aatgccagtt actgttttcc
     9781 cgtctacata cctaagggaa taacatgtat cacgacgtca gctatctgct ctcccgcctg
     9841 atcaacggcc cgctgtccct gcgccagatt tactttgcca gcagtaacgg tccggcgccc
     9901 gatcttgcgt atcaggtcga ttttccacgg ctggaaatag tgctggaagg tgagtttgtt
     9961 gataccggcg ctggagcaac gttagttccc ggcgatgtgc tgtacgttcc cgctggtggc
    10021 tggaattttc cacaatggca agcccccgct actaccttta gcgtgctgtt tggcaaacag
    10081 caactcggct tcagcgtcgt gcaatgggat ggcaaacagt atcaaaatct ggcgaagcaa
    10141 cacgtcgccc gacgcggccc gcgcattggt tcttttctgc tacaaacgct caatgaaatg
    10201 caaatgcagc cacaggagca gcaaacggca aggcttatcg tcgccagcct gcttagccac
    10261 tgccgcgatt tgctcggcag ccaaatacag accgcctcac gcagccaggc tctattcgaa
    10321 gctattcgtg attatatcga cgaacgctat gcctccgcgc ttacccgcga atctgtcgca
    10381 caggcgttct atatttcacc aaactatctc tcgcacctgt ttcaaaagac gggggccatt
    10441 ggttttaacg aatacctgaa tcacacgcga ctggagcacg ctaagacgtt attgaaaggc
    10501 tatgacctga aggtaaaaga agtggcgcac gcctgcggct ttgtcgacag caactacttc
    10561 tgccgtctgt ttcgtaaaaa caccgaacgc tcgccgtcgg aatatcgtcg gcagtaccac
    10621 agccaactga cagaaaaacc gactactcca gaataagatg ggtttgctgc ggcgcagaaa
    10681 gcactttacg taccgcgctc atcacccgct gcggctcacg caggaacgcg tagatactgc
    10741 attgcacgta gcgacaatgt tcaaatcgct cggcaccagc aagctcgata tccgtaatca
    10801 acagcgcaac atccgcccgg cggatatcct cgtccgctaa acgattctcc gttcccagcg
    10861 cgccctgagt ttcaatgctg acgccccact tctctaactg gcacaacttt tccagccgtt
    10921 ccgccgccat ataagtatgc gccacgccac tgacgcaggc ggttactgcc accaggtatg
    10981 ccattttaac ctcccacggt aacctgaaat ccggcccgtt ctgccatttc gcgcatcgtt
    11041 gccacatcgg ctgacgacgg cgcagaaacc tctttcatcg accatgtttt ccccagcaga
    11101 cggtatttcg gttcgccgta ctgatgaaac ggtaacagat ggatctgcct gatattcagc
    11161 gggatcagca cgtccagcgc ctgctgcata ttctcccggc tgagtgtgaa accggggatc
    11221 agcggcaaac gcgggatcac gttgacacct tcactcacca gcaaacgcag attctccagc
    11281 acgcgtggca ggttcatctt caccacatcc cgcgcctgag tcgcgtccat aatttttaaa
    11341 tcgaacaaca cttcatcgca caatttcgcc agcggtaaca gcttgctggc tggcgcgtct
    11401 ccggcagttt cgatggcgca tgacacgccc cacagccgca gtcgctgtaa aaaacgggta
    11461 gcaaactcgg cctgcattaa cacttcgccg ccagaaagcg tgacgccgcc gccggacgtg
    11521 cgaaagaaaa tgtcatcttt catcacttcc cgttccagag cgtcaaggct gatatcgcga
    11581 ccaatccgtt caaacgcacc ggaggggcat tcatccgcat cacgcaaaca tttcgcacag
    11641 tgcagacatt tcgcctctct gcgtaccgtc tggattttgc cggagatcga ctccggatta
    11701 gcacaccacg ggcaaagatg cggacagcct ttaaaaaaga ccaccgtacg aatgccctcg
    11761 ccgtcattca gtgaataacg ctgaatgttg aaaatgcgcg ccacatcatc gcggcgcgtt
    11821 tccacaacgt tacagctgat gcgctgtccg gcggatgatg tcatcctgga tctccttcga
    11881 cagttcgaca aagaaggcgc tgtatccggc aacgcgcacc accagcccgg cataatcttg
    11941 tgggcgctgt tgcgcttccc gcaacgtgtc ggcgttcacc acgttaaact gaatatgctg
    12001 caacttaagc tgggtaaacg cccgtaagaa gtcggccagt ttgcgtaatc ccgcttcacc
    12061 ttccagggtc gccggagtga atttcacgtt cagcaacgta ccgttagaca gcagcgtgtt
    12121 atcgagctta ctgactgact tcagtaccgc tgttggccct tgtgcgtcct ggcccagcat
    12181 aggtgacaag ccgccgtctg ccagttgttc cccggcaaaa cgaccgtctg gcgtcgcgcc
    12241 aaccaccgat cccaacggga cgtgagcaga aacggtatac gatcccggcg tgaagtaacc
    12301 gccgcgcggg ttctggtatt tttccacttc tttgcagtag tggcgtaaca gttcggcgct
    12361 gatgttatcc acctcgtcga tatcgttacc gtatttctcg aagcggttaa ttaagcgagc
    12421 acggactttt tcgccttctg gcgttgcaaa gttggctttt aataccgaca gcaattcgtc
    12481 aaaacttaaa cgctgttgct caaaaaccat ccctttcaac gcatggagag aatcgctcag
    12541 gttggcgata ccgatcccct gtacgccgga gaagttataa cgcgcgccgc catcggtaat
    12601 atcgcgacct ttttccagac aatcgctgat aaaagacgag agcagcggta caggtgccca
    12661 gtcgcgatgg ccgatatcac aaatattgct gccctcaacc atcagggtga tgtagtggct
    12721 gatcttggcg cggatctgct ccagtaaacc ttcataagtc agcgcagcat tgccttcatt
    12781 ttcatgcagg cagatttcca tcactttcag cagattaaac atcgcgatgt catgcaagcc
    12841 gtaggttctg ccgggaatag ataattccac acagcccact acggaatagt cgcgcgcatc
    12901 ttccagcgaa acgccacggt tgaggaacgc tggtaccacc acttcatcgt taaagatttg
    12961 cggaataccg gtgccgaggc gaatggtttc ggcggttttc atcaggaacg gcgtgtcgat
    13021 caaggcgtta gtgcgcacgc cgaggttcgg ttgcggtaat tgcacgcttt gataagcgtc
    13081 aaggcaaagg aacgaaagca cgttcaccgc gctacgtccg ttctcggtta acccgccgag
    13141 cagtgcggta tagccggtcg ggaaacctgc gaaataacgc gcgctgctgg tggagcgcaa
    13201 cagcacgatg tcgttgcatt tcacccataa agattcgagt agttctttca ggaacgccgg
    13261 atcttcgccc tgggttaaag atgcctgata gaacggcaac atatactggt cgaagcgccc
    13321 caacgatagc gaactggcgt tggattcgta ttgcagaatg atgttcatgt accagaataa
    13381 ctggcacgcc tgccagaacg tctgcggctt atgttcggcg ttatggcggg agatctccgc
    13441 aatagtcagc agttcttcgc gacgctgggc atctgtgcag tttgccgcca ttgtttccgc
    13501 cagttcagcg taacgcagaa tgtgtttctg cgaggcttcc agcagtaaca gcgcggcctg
    13561 ataaaagtga ttctccggct gttgctgaca atgttgctgc atctgcgcca ccagctcccc
    13621 cagcccgtga ttcagcaggc gtgggtaatc aataataatg tgcccctggc ctttatctgt
    13681 ctggttgatg ctgaaaatct gcgtgctggt cgcggctttt acttcatctg tcatctgccc
    13741 gttgatgaaa tctttcatcg aacgtttttc ccagtacggg aacaactctt cacggtagat
    13801 acgtttatct tcttcactga tagcaaagcg gtcctgcgga cgcgtcggga attgatccag
    13861 ctctttcagc agccagtaag ggtccatttc cggcgacata atcccagcgc gcggttttac
    13921 ggtgcggtta ccggcaatca gttcttcatc acgaatcgaa atttcaacat gttcaaggat
    13981 atacgctgtt gctttcgccc ggcgcaatat caccggttcg ccttcggttt gccgatggct
    14041 ggcggtataa agcagcgccc gctccagcga gatttcacgg gtattggcaa acagtgcagt
    14101 tttgaggcga gagatacgat tcgtcatgag gaactcctgt gggaaagcga acccgccctt
    14161 gcgaggggag aagaacttaa gcggtttgcg ccaggtgagc ttcaattttg ctcataatgg
    14221 cgtcagcacg ttttactgca tcgctgatgt tgacgcgcac gatggtcttg ccagcaaaac
    14281 gctcttcaaa cttgatgccg atatctttgg tcagaatcac catatcagcg ctcgccacat
    14341 cttccgcagt cagttcattt tccagaccaa ttgacccctg ggtttctact ttcacttccc
    14401 agcctttcgc tttcgcggca ctttccagcg cctctgctgc catataggta tgggcaacac
    14461 ctgaaggaca tgcggttact gcaataattt tcgtcatggc tcgttcctca attaattaat
    14521 ttcaaaatcc aaatccaggt cgtcttcttt ttcatcagtc gaagacccat ttttacgcgc
    14581 cagacttttc agcacgttca cacaaacagc cgtcaccact gctccgacag ccactgctgc
    14641 gatataaccc agcttgcctt caaccaccgg cagcacaatc agaccacccc agcctgcata
    14701 gcactgcgca ccgaccagcg ccgcagttac tgcaccacaa actgaaccga ccatgatgga
    14761 aggaatgaca cgcagcggat cggcagcggc gaaaggaatc gccccttcag taacgccaac
    14821 acagcccatc accagtgccg ctttgccggt ttcgcgctct tctgcggaga aatttttacg
    14881 accaatcagc gtcgccagcc ccatccccag cggcgggata caaataccca cggcagcgat
    14941 ggccaccacg gtataaacac cctgagcaac gcaaatcagc atgaaggcat aggccacttt
    15001 gttaaccgga ccgcccatat cgaacgccag catcagaccc atgatcaccg ccagcataac
    15061 aatgctgccc tgctgcatcc cctgaagcca ctgagtcagg ctgttggtca acgcccctac
    15121 aggctcgccc aagccccaca tcatgatgcc tgcggtaatc agtgtgccga cgataggaat
    15181 aatgaagata ggcatcaccg agcgcagaac tttatgcacc ggaattttct tcaggtaatg
    15241 caccacgatg ccgccgataa tcccggcgat cagtgcacca aagaagcccg caccaaagct
    15301 gttaccaacc caggcaccga tagcgcacgg agccagcgca gaacgctctg caatggaata
    15361 accgatgtac gctgcgagga aaggcaccat cagcgtcaag cccgcaacgc cgatatcaaa
    15421 cagttttttc agatttggat cggctacggc atccggcact gcgcctttgc catacaacat
    15481 gacggaaacc gccagcaaaa taccgcccga taccacgaag ggaatcatgt gtgaaacgcc
    15541 cgtcattaaa tgctgacggg tatttttcag gatctgcacc aactcattca taagctgctc
    15601 ccgggcttgc gaaagcccat gtccatattt ttgatggagg aaacaatagg caatcgcagg
    15661 cggagcaaac agtggataaa aaggccattt actggacttt tgtaatatca gtacaaaaat
    15721 gcgatccgcc tcataacttg cgataaaccg gaagcatgcc gcaaaacagc gtcttgtgag
    15781 acgcgtcgca aattgacggt tacattacat ttgtccagct tttggcagat ttgtcacatg
    15841 gcaggccgtt gccgtgcgct cttatcctgt aaacagaaaa ccgtaccggg agagaggcaa
    15901 tggccctgat tgtggaattt atttgtgagc tacctaacgg cgtacatgca cgtccggcaa
    15961 gccacgttga aacgctgtgt aatacttttt catcacaaat tgagtggcat aacctgcgca
    16021 ctgaccgcaa gggcaacgcc aaaagcgccc ttgcgctgat tggcaccgat acgctggcgg
    16081 gcgataactg ccagttactg atttccgggg ccgacgaaca ggaagcgcac cagcgtttaa
    16141 gccaatggct gcgcgatgaa ttcccccact gcgatgcgcc gctggcggaa gttaaatctg
    16201 acgaactgga accactgccg gtttcactga ccaatctgaa tccgcaaatt atccgcgccc
    16261 gcaccgtgtg cagcggtagt gcaggcggca ttctgacgcc gatctcttct ttagatctca
    16321 atgcgctggg taatcttccc gcagccaaag gcgttgacgc cgagcaatcc gcactggaaa
    16381 acggcctgac gctggtactg aaaaacattg agtttcgtct gctggatagc gacggtgcca
    16441 ccagcgcgat tctggaagcg caccgatccc tggctggcga tacttccctg cgcgaacatt
    16501 tactggcagg cgtcagcgcc ggattaagct gcgccgaagc gattgtcacc agtgcgaatc
    16561 acttttgcga agagttcgcc cgttccagca gcagctacct gcaagaacgt gccctggacg
    16621 tacgcgacgt ctgcttccag ttactccagc aaatctacgg tgagcaacgc ttcccggcac
    16681 cgggcaaact gacgcagccc gccatttgta tggctgatga actgactccc agccagttcc
    16741 tcgaactgga taaaaatcac ctcaaaggtc tgttactgaa aagtggcggc accacctcac
    16801 ataccgtaat tcttgcccgt tcgttcaaca ttccgacact ggttggtgta gatattgatg
    16861 cccttactcc gtggcagcat caaacgattt atatcgacgg caacgctggg gcgattgtgg
    16921 ttgagccagg agaagccgta gctcgttatt atcagcaaga agcccgcgta caggacgccc
    16981 tgcgtgagca acagcgtgtc tggctgaccc aacaagcccg taccgctgac ggtatccgca
    17041 ttgaaattgc cgctaacatc gctcactccg tggaagcgca ggccgcattc ggcaatggtg
    17101 cggaaggcgt tggtttgttc cgcactgaaa tgctctatat ggatcgcacc agtgcaccgg
    17161 gcgaaagcga gctgtacaac attttttgtc aggcgctgga gtctgccaac ggacgcagca
    17221 ttattgtgcg cactatggac attggcggcg acaaacccgt tgattatctg aacattccgg
    17281 cagaagctaa cccgttcctc ggttatcgcg ctgtgcgtat ttatgaagaa tacgcctcgc
    17341 tgttcaccac acaactacgg tcgatcctgc gcgcctccgc tcacggcagc ctgaaaatca
    17401 tgatcccgat gatctcctca atggaagaga tcttatgggt gaaagaaaaa ctggcggaag
    17461 ccaaacagca actgcgtaac gaacacattc cgtttgatga gaagatccag cttggcatta
    17521 tgctggaagt accgtcggtg atgttcatca tcgatcaatg ctgcgaagag attgatttct
    17581 ttagtattgg tagtaatgac ctgacgcaat atctgctggc agtggatcgc gataacgcta
    17641 aggttactcg tcactacaac agcctgaatc cggcattctt gcgggcgctc gattacgccg
    17701 tgcaggcggt gcatcgccag ggcaaatgga ttggtctgtg cggtgagctg ggagcgaaag
    17761 gttccgtgct gccgttgctg gtcggcttag ggctggatga actcagcatg agcgcaccat
    17821 caattccggc ggcgaaagcg cggatggcgc aacttgatag ccgtgagtgc cgcaagttgc
    17881 tcaaccaggc aatggcctgc cgtacttcgc tggaagtgga acacctgctg gcgcaattcc
    17941 gcatgaccca acaggacgca ccgctggtca ccgccgagtg catcacgctg gaaagtgact
    18001 ggcgcagcaa agaagaagtg ctcaaaggca tgaccgataa cctgctgctg gcgggccgct
    18061 gccgctatcc gcgtaaactg gaagccgact tatgggcgcg cgaggccgtt ttctctaccg
    18121 gtctgggctt tagttttgcc attccacaca gcaaatcaga acacattgag caatccacca
    18181 tcagcgtggc gcgtctgcaa gcgccggtgc gctggggcga tgatgaagcg caattcatca
    18241 ttatgttaac cctgaacaaa cacgctgcgg gcgatcagca catgcgcatt ttctcgcgcc
    18301 tcgctcgccg catcatgcac gaagaattcc gtaacgcgct ggttaacgcc gcctctgccg
    18361 acgctatcgc cagcctgctg caacatgaac tggaactgta aaaggaaaca tcatggaact
    18421 gtatctggac accgctaacg tcgcagaagt cgaacgtctg gcacgcatat tccccattgc
    18481 cggggtgaca actaacccga gcattatcgc tgccagcaag gagtccatat gggaagttct
    18541 gccgcgtctg caaaaagcga ttggtgatga gggcattctg tttgctcaga ctatgagtcg
    18601 tgacgcgcag ggtatagtgg aagaagcgaa gcgcctgcgc gacgccattc cgggcattgt
    18661 ggtgaaaatc ccggtgactt ccgaaggtct ggccgccatt aagatgctga aaaaagaggg
    18721 tattactacg cttggcaccg cagtgtacag tgctgcacaa gggttactgg ccgcgctggc
    18781 aggggcaaaa tacgttgctc cgtatgttaa ccgcgtagat gcccagggcg gggacggcat
    18841 tcgtacggtt caggagctgc aagcgctgtt agaaatgcac gcaccagaaa gcatggtgct
    18901 ggcggccagc tttaaaacgc cgcgtcaggc gctggactgt ttactggcag gatgtgaatc
    18961 catcaccctg cccttagatg tagcgcaaca aatgctcaac acccctgcgg tagagtcagc
    19021 tatagagaag ttcgaacacg actggaatgc cgcatttggc actactcatc tctaaaggag
    19081 caattatgga ccgcattatt caatcaccgg gtaaatacat ccagggcgct gatgtgatta
    19141 atcgtctggg cgaatacctg aagccgctgg cagaacgctg gttagtggtg ggtgacaaat
    19201 ttgttttagg ttttgctcaa tccactgtcg agaaaagctt taaagatgct ggactggtag
    19261 tagaaattgc gccgtttggc ggtgaatgtt cgcaaaatga gatcgaccgt ctgcgtggca
    19321 tcgcggagac tgcgcagtgt ggcgcaattc tcggtatcgg tggcggaaaa accctcgata
    19381 ctgccaaagc actggcacat ttcatgggtg ttccggtagc gatcgcaccg actatcgcct
    19441 ctaccgatgc accgtgcagc gcattgtctg ttatctacac cgatgagggt gagtttgacc
    19501 gctatctgct gttgccaaat aacccgaata tggtcattgt cgacaccaaa atcgtcgcgg
    19561 gcgcacctgc acgtctgtta gcagcgggta tcggcgatgc gctggcaacc tggtttgaag
    19621 cgcgtgcttg ctctcgtagc ggcgcgacca ccatggcggg cggcaagtgc acccaggcgg
    19681 cgctggcact ggctgaactg tgctacaaca ccctgctgga agaaggcgaa aaagcgatgc
    19741 ttgccgccga acagcatgta gtaaccccgg cgctggagcg cgtgattgaa gcgaacacct
    19801 atttgagcgg cgttggtttt gaaagcggcg ggctggcggc ggctcacgcg gtgcataacg
    19861 gcctgaccgc tatcccggac gcgcatcact attatcacgg tgaaaaagtg gcatttggta
    19921 cgctgacgca gctggttctg gaaaacgcac cggttgagga aatcgaaacc gtagctgcac
    19981 ttagccatgc ggtaggtttg ccaataactc tcgctcaact ggatattaaa gaagatgtcc
    20041 cggcgaaaat gcgaattgtg gcagaagcgg catgtgcaga aggtgaaacc attcacaaca
    20101 tgcctggcgg cgcgacgcca gatcaggttt acgccgctct gctggtagcc gaccagtacg
    20161 gtcagcgttt cctgcaagag tgggaataac ctactccaaa ctcccggctt gtccgggagt
    20221 ttgaacgcaa aattgcctga tgcgctacgt ttatcaagcc tacgaaatct ctgcaatata
    20281 ttgaatatgc gtgcttttgt aggccggata aggcgttcac tccgcatccg gcactctgtg
    20341 ccagtaatct gaaactccca cgttgttcag gagtttttta tccgcttctg gacattttct
    20401 ccacagaaca atcgttagcc cggaaggtcg aatctgtgac tcttattttc acggacgaac
    20461 cagatgaaag cttcactggc gctgctcagc ctgctaacgg catttaccag ccactcctta
    20521 aaatctcccg ccgttccacc aaccgtggta caaatccagg ccaataccaa ccttgccatc
    20581 gcagacggtg ccagacagca aatcggcagc acgctatttt acgatccggc gtatatgcag
    20641 cttacctatc ctggcggtga tgtcccgcaa gaacgcgggg tatgttccga tgtggtgatc
    20701 cgtgcattgc gcagccagaa ggtcgatttg cagaaactgg tgcacgaaga tatggcgaag
    20761 aattttgccg agtacccgca aaaatggcag ttaaagcgcc cggacagcaa catcgatcac
    20821 cgtcgggtgc ctaatctgga aacctggttt acccgccacg ataaaacacg ccccatcagc
    20881 aagaacccca gtgactatca agcgggcgat attgtctcct ggcgactgga taacggactg
    20941 gcacatattg gggtggtgtc agatggcttc gcccgcgacg gtacgccgct ggtgatacat
    21001 aacattggcg caggcgcgca ggaagaagat gtgctgttta gctggcggat ggtggggcat
    21061 tatcgttatt ttgttaaata agcgatcgcc ccacatgaag aaaaaattca gatgcttttt
    21121 acactgacag cttctttttt cttacggctc accagcgcca gtgccagcac aatcagcaca
    21181 ataccgctac cttcaacggc ccccggattc tcaccgagca gccaccagga aaagagtacg
    21241 ccgcaaacgg gaacggccag cgtgcttaag ctggcaatac tggcaggcaa gtttttcaat
    21301 acaaacaacc ataagctcca cgccagcgcc gtcgccagaa tcgcactgta ggccagcgcc
    21361 cagaacacgg tgggctgcca gtcaatttca cgttgcggca ccagtaaagc gaccacactc
    21421 atcaccagcg ccgcatagag catctgccag gatgttaacg ataataaatc cacgcgcgga
    21481 tgacgggtgt ataggcgttt agcaacaatc gcgctcgccc cccaactgac gccggagagg
    21541 attgccagca tggcactttt cattgaagag aaatcgagtt gccacggctg caacaccaaa
    21601 aataagccga aagcggcaat cagtatcgcg aaatattgcc cacgtcgcag gcgttcaccg
    21661 agaaacaacg cggcgaaaat caccacccag aacggcatgg tatagcttag gatcgccacc
    21721 ttccccgctc cgccgctgac cagcgcccac tgcgccaggc caaccatccc gcaggtttgt
    21781 aacagggcaa tggctaaggt gtatttaaac ggtgtcgggc gcattccgcg accacgtaat
    21841 aaaaggacga tgaataaaac gagagcgccg aaaatgcagc gtaaggcggt aaagtcgaag
    21901 gcaccgatgt agctggtgac ttgcttcatg aaaatccagc tataactcca gataagtgtg
    21961 agcacaacca ggccactgat tgccagtggg ttgctctttc ctgcggcaga catgagagat
    22021 ccagtgtgta gttttcctgt ggacactata cgccggtaaa gaaacttcag cgactgcctg
    22081 aaagacagtc gctgaagaag gtcagattac agcaggtcga aacggtcgag gttcatcact
    22141 ttcacccatg ccgccacgaa gtctttaaca aacttctcgt gggcatcgct gctggcgtaa
    22201 acttccgcta ccgcacgcag gacagagtta gaaccaaata ccagatccgc acggctggct
    22261 gtgtatttca cttcgccggt ttcacggtca cgaccttcga acagctcttt cgattcgtca
    22321 gtcgctttcc actcgtaacg catatccagc aagttcacga agaagtcatt gctcaatacg
    22381 ccaacgcggt cagtgaagac gccgtttttg ctgccatcga agttggcacc cagtacacgc
    22441 atcccgccca ccagcgcagt catttccggc gcggtcagcg tcagttgctg tgctttgtcg
    22501 atcagcagtg actcggtggt ggaaacatcc agacgagcgc gatagttacg gaaaccgtca
    22561 gcaattggct ccagcagctc gaacatctca atgtcagtct gatcctgacg cgcatcaacg
    22621 cggcccggcg caaacggtac atgaatgctc aaacctgcgg cgcttgcggc tttctcaaca
    22681 ccaaccacac cagccagcac gatgatatcc gccagcgagg ctttaccaga ctctttctgg
    22741 attttctcca gaacaggcag agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc
    22801 ggcattaatg ccagacgcgc accgttggca ccaccgcgtt tgtcgccacc acggaaggta
    22861 gaagcagaag cccaggccac cgataccagc tcactaacag acagaccaga atccgcaatc
    22921 gcgaatttca gatcgataat gtcctgctcg gtcgggttgt agatcggctg cggcagcgga
    22981 tcttgccaga tcagatcttc tttcggcact tccggcccga tgtagcgaga tttcggcccc
    23041 atatccctgt gcgtcagttt gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga
    23101 tcgttgagga aacgacgaga gatcttctcg aactcaggat caaaacgcag cgtcaggtcg
    23161 gtcaccagca ttgtcggttt acgtttcttc gacggatcga acggatccgg gataatttcc
    23221 ggtgcgtcta ccgcttcgaa ctggattgct ccagccggac tgcgggtctg tacccactca
    23281 tacttgaaca gattctcgaa gaaatagttg ctccactggg tcggcgtctg ggtccagact
    23341 acttccagac cagaggtaat ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag
    23401 cctaaacctt gttcttcaat cggtgcagct tctggatcag gacctacgtt tgatgtcgga
    23461 ccggcaccgt gggttttacc cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg
    23521 tcgttcatgc ccatgttgcc gaaggtcgcg cggatagctg ctgccgcaga aagcggttcg
    23581 ccgctgtgat ccgggccttc cgggttaacg taaatcaggc ccatctcggt tgcacccagc
    23641 ggtgctttcg ccagcgcttc cggatgacgg tgagtcagcc aggctttttc atcaccccag
    23701 ttaacatcca gatccggttc ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg
    23761 aagccggagt tttccagcgc cacgttacct gcgaggataa acaggtcggc ccaggagatt
    23821 ttctgaccat atttctgttt gattggccac aacaggcgac gcgctttatc gaggcttacg
    23881 ttatccggcc aggagttcag cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt
    23941 ccatcgattg aacggtaagt acctgcgccg tgccaggcca tacgaataaa cagaccggcg
    24001 taactgcccc agtcggcagg ccaccacggc tgagattctg tcagcagggc tttcagatct
    24061 tttttcaggc cgtagtaatc taatttgctg aattctttgc ggtagtcaaa gtcctcaccc
    24121 agtgggttag aacgattaga atgttggttt aacaggtcaa cacggagttg atttggccac
    24181 cagtcgcgag tggttgtgcc cgcccccgcg ctctggtcgt gaccgccctg atggaacggg
    24241 catttgccag tggctgtggt gttatgaata tcgtctgacg tgctcatcaa tgtgctcccc
    24301 tctacagtgt taccgttacg atacacagcg ttagagagaa gttataattg aattaatcca
    24361 cggatgcgat agttgagatc ttacatattg ttggttaaag agatgtagat caaattgatc
    24421 gtaattaagc ttttttgatt atgtccgaat tcggacaaat gctttataaa aaagggtttt
    24481 aggttcatat ccttcaagta atgagggaga aaacaaggct gataacctta ttttcccgcc
    24541 ctcatttcga ggcagcattt tgtgctctgt ttaaaatttg tgatcactgt gtgattttca
    24601 caaaagccac actatttata aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact
    24661 catttcagcg cggttaagcg tatagaagtg gaaatctttc actccttcac ggcttaaaat
    24721 cttcaccata tccatggcaa tattcgctcc caccagtttg cgggtttcgg catcgtcgtc
    24781 cagcccgtca aacatttgtg ccatccacgc cggaatacgc acgttggtca tatcggcaaa
    24841 tttcttcgct tgtttaaagt tcgagaccgg caaaattccc ggaataattt ccacatcaat
    24901 acccgccgat acgcagcggt cacgaaaacg caggtagctt tcgacatcaa agaagaactg
    24961 agtaatcgcg cggttggctc cggcatcgac tttgcgtttc aggttgagca aatccgcctg
    25021 agcgcttttt gcttccgggt gaacctccgg atacgccgcc acggagatat cgaaatctgc
    25081 cacttctttt aacagcgtca ccaggtcaga agcatacatt tccggcttac cacttcccgg
    25141 cggcagatcg ccacgcagcg ccacgatatg acgaataccg ttattccagt agtcgcgtgc
    25201 aatggtgcgc agctcgtcgg gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag
    25261 accagtgcga tctttaatgc ctttaataat gctgtgcgta cggtcgcgct cgccggagtt
    25321 cgcgccatag gtcaccgata caaacttcgg tttcaggctg ctaaggcgat cgatggagtt
    25381 ccacagggtc tgctccattt cactggtacg cggcgggaaa aactcgaacg aaacgttaat
    25441 ctgcccctgg acttctgcca ggctctgatt cagggcatcc cgctggctgg cgtgaaaaaa
    25501 gctcatacct tacctcatca atcgcttgta gttatatgtt gtgttgtgca catctatccg
    25561 tttagacgtc cagatgtaaa gatgaaggaa aagccgaagg gcgtcaaccg aaaaatgacc
    25621 aatgatggtg aggaaaaatc agggaagatg aaaaaacttc aggatggtaa gaaaaagaaa
    25681 atccggtatg caaagcaaac cggactgcaa atataaacta accatgtact tattgcttct
    25741 tgccggattc gtcgtaaacg cttaatccgg cctaatccta tgataattca atatatttca
    25801 ttagaaacca taaacccgaa aacatgagta ccgggcatta ttaaatttct gaaattacaa
    25861 caactgtgcc agccggttga tatccgactg aatcgccccg gcggtgacgt cgcgccccgc
    25921 gccaggccca cggatcacca gcgggttgtc gcgataccag cggctttcga tggcaaagac
    25981 gttatcgcac ggcagcagtg atgccagcgg atggtcttca cgcaccgcct ccacgcccac
    26041 acgcgctttg ccgttggcat cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg
    26101 ggccgcttcc agccgttgca ccatctgctc gttaagttca tcgccatttt caaagaaatg
    26161 gtcgatgctg ccgccttcgc aatgggcagg caccagcgac tccacgcgta cctgatccgg
    26221 ttcgatgttg taacctgctt cacgcgccag aatcaccagc ttgcgcatta cgtctttgcc
    26281 ggaaaggtca tcacgcgggt caggctcggt taacccttgc tgccacgcct gatccaccag
    26341 ctcggtaaac ggcacgctac cgtcgaattg caggaacagc caggagagcg tgccggagaa
    26401 gatcccactg atcgacaaaa tagtatcgcc gctgtcgatc agatcgcgca cggtatggtt
    26461 gatcggcaag cccgctccga cggtagcatt gtacagccag tgacgcccgg ttttttcgaa
    26521 ggcgtcgtgg atctggcgat atttattgct gtcgctggct cccgccagtt tgttggcgct
    26581 gatgacgtgg aaaccgtggc tggcgaaatc aagatactga tcagcaagct gctggctggc
    26641 ggtaacgtcc agcaccacta aatcatcata cggatgggcg cgcatccaca ggaacaacga
    26701 ctcttcatcc tgctcaaccg cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag
    26761 cccgtcatag ctcaacaggc tgcggcggct gtccaccaca cctgccagca caaactcaaa
    26821 gccggtacgt gccgaaagcg tgctctgctc acgggcgaac agttccagcc aacgggaacc
    26881 gatattgccc ttaccgaaca ataccaggcc gatgcgtttt tctgcgcgga agacggactg
    26941 atgcagcccc tgaatcaggc tttcggtcgg gccggtgcgc agtactgcca ccaggctgat
    27001 gccgtcatcg gactgccagg taaattcgac cggctggcct ttcagttgct gccagaagcg
    27061 gtggcaatgc agcgggttac gggtgacgcc tgcaccgacc atcgccacca gcgccagccc
    27121 ctgacgcagg cgcagttcgc caggtaatcc cgcttcgtcg aggattttca gcgcactgtc
    27181 ggccacttct gaggtgtagc aaaattgcag caactggcga tcgttatgta cgccaaccgc
    27241 cagcgggcgt acctgcgcgc gtttcaggat ttggtcgatc tctttatgcg ccagtttgaa
    27301 atcctgactg gcgggcacct gaaactcaat caaacagaca tcatcgtggc tggtgacaat
    27361 acgcgcacca gtaccggagg ccagcacgcg ttcaatgcgc gtggaacctt gatccggcgt
    27421 gtagctacag cgcagttgca ggtcgatttc gctgccagaa accggctgta aagtacgggc
    27481 gtgaagaacg ggagccgcca ggcgcgccag ttcgctggcc tcatccagac gcagcaacgg
    27541 cagcaggcag gcatctttca ctttacgcgg gtcggcactg tataccccgg cgacgtcgct
    27601 ccagatggtt acgcgagaaa cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga
    27661 accgttacgc cccagcagca ccgtttcacc ggcgttgttg cggctgataa atccggtcac
    27721 caccagacgt ttgcccggat gttgcaccag cagctgttgc agcaacgggt aagaaagccc
    27781 ttcatcaacc tgcggttgtg cggcgcgttc agcgcgtaaa aactcgcggg catcaagcca
    27841 ggccgctggc agcccttgtt gattaagtac cgcagacatc aggcgtgccg accacacttc
    27901 accgtgcccc accacttccg catacactgc gtcgttaata ccgctgtcga gcagcgccgc
    27961 caggcgctca aggtcgctga caaaagcgct aatgaggcta tcggcttctt cagcgggtag
    28021 cagaccgcta atcagatcgc actgatagcg acgcagcgtt tgttgaacct gatgcgcaga
    28081 gagacgatcg gtctggctta gtttcaacca gttaatcaac tggttagtgg tgctaccggc
    28141 ggcggaaacc accatcatat cgtcaggctg agagtactcc gccataatgc ccgcgacacg
    28201 caaataacac ttcacatcag ccagactact gccaccaaat ttatgcagct gacgaccttt
    28261 cgcccctgcc tgcgcaatca cactcatttt taccccttgt ttgcagcccg gaagccattt
    28321 tccaggtcgg caattaaatc ttcgccatct tcaataccgg tggagatacg cagcagcgtc
    28381 tcggagatcc cggcggcagc acgcgcttct ggtgccatgc ctgcatgtgt catggttgcg
    28441 gcgtgagaga ttaaactttc cactccccct aatgattccg ccagcgtaaa caacgacagt
    28501 ccacccagga aacgacgcag cgtctgctca tcgccatcca gttcaaaact caacattgcg
    28561 ccatagcctt tttgctggcg cgcggcaatt tcatgcccct gattttccgg cagcgacggg
    28621 tgatacagtt ttttcaccaa cggctgggtt tgcagatatt tcacaatcgc ctgcgcgtta
    28681 cgctgggcca actccatgcg cggcaccagc gttcgcaacc cgcgtagcag cagatagctg
    28741 tcaaacgcgc cgcccgtcac gccaatattg tttgcccacc aggcaagttc agtgacaacg
    28801 tccgggtctt tagcaatcac cacgccggcc accacgtctg agtgaccgtt cagatatttc
    28861 gtgcatgaat gcaacaccag atcggcacct aatgccagcg gattttgtaa tgccgggctt
    28921 aagaaggtgt tatccaccac gctcaccgcc ccgacttccc ttgccagatg gcagattttc
    28981 gcaatatcca cgacgcgtaa caatggatta cttgggcttt ctaccagtac cagtttgggt
    29041 ttatctgcca gcgctgcccg taatgcctgt tcatcgcctt gatcaacaaa caacacgcga
    29101 tagcaaccgc gtttcgccag gctgtcgaac aggcgatagc taccgccgta gcagtcgtgc
    29161 ggcgctacca gcagatcgcc aggtttcaaa aagacggtcg ttaccaggtg aatcgcggac
    29221 atgccggtat tagtaagtac tgcaccagca ccgccttcca gttctgccag tgctcgctga
    29281 accacatcgc gcgttgggtt gccacgacgc gagtaatcat gcgcgcgcgg ttcattaaat
    29341 ccggtaaagt tataggtact ggaaagatgg atgggtggga caacgcaacc atactgttcg
    29401 tcgtcattta acccgctacg cactgcgatg gtggcctgtt tacgcgtcat gtgatgatgt
    29461 tccctgggct ttattggtga aatgtcaggc accagagtaa acattgtggt aatggacgtc
    29521 aatacatctg gacatctaaa cttctttgcg tatagattga gcaaatccca aatagccgtt
    29581 aaaattatat gcattatcac gccagcaggt ggattacccg atgtcacggt agcgcctgta
    29641 cggtaaacta tgcgggttta cggccagtgc ccacattaac tgtgtggtct ggtctcaatt
    29701 tattgacgaa gaggattaag tatctcatgg ctgaatggag cggcgaatat atcagcccat
    29761 acgctgagca cggcaagaag agtgaacaag tcaaaaagat tacggtttcc attcctctta
    29821 aggtgttaaa aatcctcacc gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg
    29881 ctaccaacag cgagctgctg tgcgaagcgt ttctgcatgc ctttaccggg caacctttgc
    29941 cggatgatgc cgatctgcgt aaagagcgca gcgacgaaat cccggaagcg gcaaaagaga
    30001 tcatgcgtga gatggggatt aacccggaga cgtgggaata ctaaagcgga aaagagcggc
    30061 gcggagtgga accgcctgat gcgctacgct tatcaggcct acgtcatatt gcaatttatt
    30121 gaatttgcac gaacttgtag gccggataag gcgttcacgc cgcatccggc ataaacaacg
    30181 agcacgttgt ctgcgaccca ccgcttttta tacatggacg tttaactatg aaaaacaggc
    30241 tgctgatcct cagcctgctg gtttctgtac ctgcctttgc ctggcagccg caaactggcg
    30301 acatcatctt tcaaatctct cgctcatcgc aaagtaaagc gatccaactg gcaacccatt
    30361 ccgattacag ccacaccggt atgctggtga tacgcaacaa aaagccctac gtttttgaag
    30421 cggtcggccc ggtgaaatac accccgctca agcagtggat cgcccatggt gaaaagggca
    30481 aatacgtcgt tcgccgcgtt gaaggcggac tgagtgttga acaacagcaa aaactggcgc
    30541 aaacggcaaa acgttatctc ggtaaaccgt atgatttcag cttctcatgg agcgacgatc
    30601 gccagtactg ttcggaagtg gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg
    30661 gcgaacagca gaagttgaaa gagtttgatc tcagcaaccc gctggtccag gcgaagctca
    30721 aagaacgcta cggtaaaaat attccgcttg aggaaacggt agtttcacca caagccgttt
    30781 tcgacgcgcc acaactcacc acggttgcca aagagtggcc gctgttttcg tggtaatgga
    30841 tgatgaaaaa aataattccc gccgtagtct gtttgttgct ttgtagttgc acgacctttc
    30901 cgcaagctgt cgctccggta aacggtgatg cctccaatta gttgaattaa ggatataata
    30961 ctcgctttta aggttgtttc atggtcacag ttaaccttca ttgcccccgt tgccagtccg
    31021 ttcaggttta ccgtcacggg cagaacccaa aaagacatga tcgattccgc ggccgtgact
    31081 gtcaccgggt atttcagctc agttactgtt acgagccccg taaaccggga gttaaagacc
    31141 aaatcgctga aatgactttc aacggtgccg gggttcgcga tactgcccgt acgctgaaaa
    31201 tcggcattaa tacagtcatc cgaactttaa aaaactctcg ccacgaagaa taacgtcatc
    31261 cccggtcgct catgctgatg tggcactcat ctgcaaactt agtcagcaat ggagctttgt
    31321 cggcagtaaa gcgcgacagc actggctctg gtacgtgtac aacacgaaaa ctggtggtgt
    31381 tctggcctac acttttggcc cccggactga cgaaacctgt cgtgaattgc gggcgctgct
    31441 taccctttta ccatcggcat gttgaccact gacgaatggg gtagttacgc tagagagtta
    31501 ccgaaggaga agcatctgac tggtaagatt tttactcagc acattgagcg taacaatctg
    31561 accctgagaa cccgaatcaa acgtctggcc cgcagaacca tctgtttctc gcgttctgta
    31621 gaacttcatg aaaaagtcat cggtgctttt atcgaaaata tatgttctac taattggagt
    31681 catcaccctt ctgataagct ttccttggat gattaccaga ct
//
LOCUS       DAZFYC010000049        31349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFYC010000049 DAZFYC010000000
VERSION     DAZFYC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.049"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(251..514)
                     /gene="cedA"
                     /locus_tag="ACHKW5_002494"
     CDS             complement(251..514)
                     /gene="cedA"
                     /locus_tag="ACHKW5_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO7511867.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            697..2958
                     /gene="katE"
                     /locus_tag="ACHKW5_002495"
     CDS             697..2958
                     /gene="katE"
                     /locus_tag="ACHKW5_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO7511868.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDSTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3005..3763)
                     /gene="chbG"
                     /locus_tag="ACHKW5_002496"
     CDS             complement(3005..3763)
                     /gene="chbG"
                     /locus_tag="ACHKW5_002496"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO7511869.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLNV"
     gene            complement(3776..5128)
                     /gene="celF"
                     /locus_tag="ACHKW5_002497"
                     /gene_synonym="chbF"
     CDS             complement(3776..5128)
                     /gene="celF"
                     /locus_tag="ACHKW5_002497"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7511870.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5233..6075)
                     /gene="chbR"
                     /locus_tag="ACHKW5_002498"
     CDS             complement(5233..6075)
                     /gene="chbR"
                     /locus_tag="ACHKW5_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO7511871.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6083..6433)
                     /gene="chbA"
                     /locus_tag="ACHKW5_002499"
     CDS             complement(6083..6433)
                     /gene="chbA"
                     /locus_tag="ACHKW5_002499"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO7511872.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6484..7842)
                     /gene="chbC"
                     /locus_tag="ACHKW5_002500"
     CDS             complement(6484..7842)
                     /gene="chbC"
                     /locus_tag="ACHKW5_002500"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO7511873.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7927..8247)
                     /gene="chbB"
                     /locus_tag="ACHKW5_002501"
     CDS             complement(7927..8247)
                     /gene="chbB"
                     /locus_tag="ACHKW5_002501"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO7511874.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8546..8884)
                     /gene="osmE"
                     /locus_tag="ACHKW5_002502"
     CDS             complement(8546..8884)
                     /gene="osmE"
                     /locus_tag="ACHKW5_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO7511875.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9086..9913
                     /gene="nadE"
                     /locus_tag="ACHKW5_002503"
     CDS             9086..9913
                     /gene="nadE"
                     /locus_tag="ACHKW5_002503"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO7511876.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10143..11030
                     /gene="cho"
                     /locus_tag="ACHKW5_002504"
     CDS             10143..11030
                     /gene="cho"
                     /locus_tag="ACHKW5_002504"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO7511877.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10990..11565)
                     /gene="ves"
                     /locus_tag="ACHKW5_002505"
     CDS             complement(10990..11565)
                     /gene="ves"
                     /locus_tag="ACHKW5_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO7511878.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFHWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11768..12253)
                     /gene="spy"
                     /locus_tag="ACHKW5_002506"
     CDS             complement(11768..12253)
                     /gene="spy"
                     /locus_tag="ACHKW5_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO7511879.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12583..13551)
                     /gene="astE"
                     /locus_tag="ACHKW5_002507"
     CDS             complement(12583..13551)
                     /gene="astE"
                     /locus_tag="ACHKW5_002507"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO7511880.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13544..14887)
                     /gene="astB"
                     /locus_tag="ACHKW5_002508"
     CDS             complement(13544..14887)
                     /gene="astB"
                     /locus_tag="ACHKW5_002508"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO7511881.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14884..16362)
                     /gene="astD"
                     /locus_tag="ACHKW5_002509"
     CDS             complement(14884..16362)
                     /gene="astD"
                     /locus_tag="ACHKW5_002509"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO7511882.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16359..17393)
                     /gene="astA"
                     /locus_tag="ACHKW5_002510"
     CDS             complement(16359..17393)
                     /gene="astA"
                     /locus_tag="ACHKW5_002510"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO7511883.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17390..18610)
                     /gene="astC"
                     /locus_tag="ACHKW5_002511"
     CDS             complement(17390..18610)
                     /gene="astC"
                     /locus_tag="ACHKW5_002511"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO7511884.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19056..19862
                     /gene="xthA"
                     /locus_tag="ACHKW5_002512"
     CDS             19056..19862
                     /gene="xthA"
                     /locus_tag="ACHKW5_002512"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO7511885.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20029..20739
                     /locus_tag="ACHKW5_002513"
     CDS             20029..20739
                     /locus_tag="ACHKW5_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7511886.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLASEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20744..21421
                     /gene="ydjY"
                     /locus_tag="ACHKW5_002514"
     CDS             20744..21421
                     /gene="ydjY"
                     /locus_tag="ACHKW5_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO7511887.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDVTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21436..22143
                     /locus_tag="ACHKW5_002515"
     CDS             21436..22143
                     /locus_tag="ACHKW5_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7511888.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            22143..22691
                     /locus_tag="ACHKW5_002516"
     CDS             22143..22691
                     /locus_tag="ACHKW5_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO7511889.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22701..23867
                     /locus_tag="ACHKW5_002517"
     CDS             22701..23867
                     /locus_tag="ACHKW5_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7511890.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            23840..25375
                     /locus_tag="ACHKW5_002518"
     CDS             23840..25375
                     /locus_tag="ACHKW5_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO7511891.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25375..26028
                     /locus_tag="ACHKW5_002519"
     CDS             25375..26028
                     /locus_tag="ACHKW5_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7511892.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26095..27402
                     /gene="ynjE"
                     /locus_tag="ACHKW5_002520"
     CDS             26095..27402
                     /gene="ynjE"
                     /locus_tag="ACHKW5_002520"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO7511893.1"
                     /translation="MKRVSQMTALAMALGLAYASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27411..28031)
                     /locus_tag="ACHKW5_002521"
     CDS             complement(27411..28031)
                     /locus_tag="ACHKW5_002521"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7511894.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28118..28525
                     /gene="nudG"
                     /locus_tag="ACHKW5_002522"
     CDS             28118..28525
                     /gene="nudG"
                     /locus_tag="ACHKW5_002522"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO7511895.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(28491..28763)
                     /locus_tag="ACHKW5_002523"
     CDS             complement(28491..28763)
                     /locus_tag="ACHKW5_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO7511896.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            28999..30342
                     /gene="gdhA"
                     /locus_tag="ACHKW5_002524"
     CDS             28999..30342
                     /gene="gdhA"
                     /locus_tag="ACHKW5_002524"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO7511897.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30459..>31349)
                     /locus_tag="ACHKW5_002525"
     CDS             complement(30459..>31349)
                     /locus_tag="ACHKW5_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7511898.1"
                     /translation="AVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAK
                     KEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHV
                     LPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIA
                     LLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIGEELAWHY
                     IMLASRKAHELFESEEDYQKNSLMGFLYRHICCYRRKLTDAELEACYRYDKQFWEHYS
                     KKCRWPIRNVPWGASSVKYS"
BASE COUNT         7754 a         8248 c         7726 g         7621 t
ORIGIN      
        1 taaatctaag tcacttacaa tataagttta agagcgacgc cacaggatga actatcaaaa
       61 atagctcatc atgattagca aaacttaacc attttaaaat aaataaacaa ttaaagaaaa
      121 aagatcactt atttatagca atagctcgtc aaaggcagct ttttgttaca ggtggtttga
      181 atgaatgtag caacgaaatg cagaatttca ggtcatgtaa ctcccggcaa aaccgggagg
      241 tatgtaatcc ttactcagtc acttcccctt cctggcggat ctgatttgcc caacgttggg
      301 cagattcagg cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacaaacg
      361 caacatattt gccacgcagc atccagacat cacgaaacga atccatcttt atcgcatgtt
      421 ctggcggcgc gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga
      481 gcggtttctt cattactggc ttcactaaac gcatattaaa aattagaaaa actgtagttt
      541 agccgattta gcccctgtac gtcccgcttt gcgtgtattt cataacaccg tttccagaat
      601 agtctccgaa gcgggatctg gctggtggtc tatagttaga gagttttttg accaaaacag
      661 cggccctttc agtaataaat taaggagacg agttcaatgt cgcaacataa cgaaaagaac
      721 ccacatcagc accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca
      781 ctggcacctg aggacggatc tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa
      841 cctaccgccc ctgggagcct gaaagcccct gatacacgta acgaaaaact taattctctg
      901 gaagacgtac gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc
      961 gccgatgatc aaaactcact gcgtgccggt aaccgtggtc caacgctgct ggaagatttt
     1021 attctgcgcg agaaaatcac ccactttgac catgagcgca tcccggaacg tattgttcat
     1081 gcacgcggat cagcagctca cggttatttc cagccatata aaagcttaag cgatatcacc
     1141 aaagcggatt tcctctcaga tccgaacaaa atcaccccag tatttgttag gttctctacc
     1201 gttcagggtg gcgctggctc tgccgatacc gtgcgtgata tccgtggttt tgccaccaag
     1261 ttctatactg aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc
     1321 caggatgcgc ataaattccc cgattttgtt catgcggtaa aaccagaacc gcactgggca
     1381 attccacaag ggcaaagtgc ccacgatact ttctgggatt atgtttctct gcaacctgaa
     1441 actctgcaca acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc
     1501 atggaaggct tcggtattca caccttccgc ctgattaatg ccgaagggaa ggcaacgttt
     1561 gtacgtttcc actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa
     1621 aaactcaccg gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc cattgaagca
     1681 ggcgattttc cggaatacga actgggcttc cagttgattc ctgaagaaga tgaattcaag
     1741 ttcgacttcg atcttctcga tccaaccaaa ctgatcccgg aagaactggt gcccgttcag
     1801 cgtgtcggca aaatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa
     1861 gcagcattcc atccaggtca tattgttcct ggtctggact tcaccaacga tccgctgttg
     1921 cagggacgtt tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc
     1981 catgagattc cgattaaccg tccgacctgc ccttaccaca acttccagcg tgatggcatg
     2041 catcgtatgg ggatcgacac taacccggcg aattacgaac cgaactcgat caacgataac
     2101 tggccgcgcg aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc
     2161 gtggaaggaa ataaagttcg tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg
     2221 cgtctgttct ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt
     2281 tttgagttaa gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggcg
     2341 catattgatc tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga actgactgac
     2401 gaccagctga atatcacccc acctccggac gtcaacggtc tgaaaaagga tccatcctta
     2461 agtttgtacg ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat
     2521 gatgaagtga gatcggcaga ccttctggcc attctcaagg ccctgaaggc caaaggcgtt
     2581 catgccaaac tgctctactc ccgaatgggt gaagtgactg cggatgacag tacggtgttg
     2641 cctatagccg ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt cattgtccct
     2701 tgcggcaata tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc
     2761 tacaaacacc ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc
     2821 aaggtcgctg accagggtga agaagggatt gtggaagctg acagcgccga cggtagtttt
     2881 atggatgaac tgttaacgct gatggcagca caccgcgtgt ggtcacgcat tcctaagatt
     2941 gacaaaattc cggcgtaaat atggataagg tcacgtcccc tggggagtga ccttaccacc
     3001 ttacttacac attaagataa ctacccaacc gataaccacg ctcagcaatt gcatatttca
     3061 acgacgctga tgtcagcact tccagctccg ttaaccgtgg gaaacagtaa gcactctgac
     3121 ggatagtatt atcgataaac gccggatgac acatcacctc cagcgaacga tcaccccgat
     3181 ggcttgcgtc atccagcact tgcaggaaca gtgcctcgct aatctcttca ccataaaacg
     3241 cgctactgaa tccctgagaa ctgcgcagat tagccggtaa atcaccagcg ttagatagcg
     3301 gctgacggtc gatgcgcaac gcaatcccct cttcagccgc aaacctggca acaatcggga
     3361 aaatctgcgg gaacatatgc acatgatgat ggctatcaag atgcgtaggt ttgcgtccaa
     3421 atagctcaat gaaacgcaaa tactgactgg caagctcctg agtaatttct tccagcggta
     3481 aagcatcttc ttctgccaac tgccagatcc attttcccag cacaccatcg cgggttaacc
     3541 ccggcatagc tgtcagtggc ttgcccatag taaggacaaa gtgcatccct atggccagac
     3601 tcggttcatc acgactcaac tgcaccgcat ggtcaatagc ctgcccattc accagcgccg
     3661 tcgttgatgt gacaatccca ttgcgacagg cctcgataat gccgtagttc tgacctttgc
     3721 ttaagccaaa atcatcggca ttaacaatca gtaagcgttc cataatcagc ctcggttaat
     3781 gtgctttttt aagctctgcg atgcagtcgg caaaatttgg cagccatttc tcgtgcgcca
     3841 gaatcatctc gcgtgccagc agctcagcat cgcgatcgga atgcaccaac ggactaaggt
     3901 ttagcgccag taacacatcg ttaaattctc cgctaagtgc ggcgttgctg gcagcaatct
     3961 cgaagccttt aatggtgtga atcagcccca tcactttatc atcgaaatgc gtaatgcgcg
     4021 gatgtggcgt cgcgccatcg cgccccagct tacaggtcat ttctaccgcc cagtctgccg
     4081 gaatattatc aatatgccca tgatgcggga tattaacgta atgttcagct tgcttgtcgt
     4141 tgtagatagc gttgatcact tcgcacgctg catcagagta ataagcccca ccgcgctgtt
     4201 ccagttcttt cggcttaact ttcaactccg gatttttata cagctcaaaa agttgtttct
     4261 cgactttctg tactacctgt gctcgtgcgc cgcctttgta gtattcgccc atttcaatag
     4321 ccagcatctc tttctgcttg aagtaataca gcagataaga acatggcagc agatttaacg
     4381 aacgaattaa gccctcacta aatggcagat cgaaaatatt ttttacagag gacgctttta
     4441 actgccctga cgccacacca tcaagcaatt cggcaaagcg cgacttgcca tttaccagca
     4501 catccttaat gaacaccata tggttgaggc cgaacagatc gatagataaa tcatcgctgt
     4561 ctttcagcat cagaacatcg cgaataaaca tcttcatgcc gatcggaata ttacacacgc
     4621 cgataaagcg tttaaatccg gtatgacgat aaacggcttc agtgaccatt cccgccgggt
     4681 tagtgaagtt aatcacccat gcattcggac aaagttcttc gacatctttt acgatgtcaa
     4741 aaatcaccgg aatggtacgc agacctttaa acagaccgcc cgcgccgttg gtttcctgac
     4801 caagataacc atgacttaat ggaatacgtt catccagttc gcgcgccggt aattggccta
     4861 cgcgcagttg ggtagtaacg aaatcagcat ctttcaatgc ttcgcggcga tccagcgttt
     4921 tataaagctt catcgggacg ccagcgttat caatcatccg ttggcagagt tcaaaaatga
     4981 tatccagttt ctctttacca tcttcgacat ccaccagcca taattcgctg accggcaatt
     5041 cgtgataacg cttaataaat ccttccagta actccggggt atagctgctc ccgccaccaa
     5101 tagtgacgac ttttaatttc tggctcataa tttctccctt cagtacagaa tactgatatc
     5161 tggcatatct gcccccccgg acataaataa tccagcaaca ggacagatat gtgaattgtc
     5221 aggtataacg acttactgat taaattcagt caatttctta cgatagctct taggcgtaaa
     5281 ggacgttaat ttcttaaacg ttttaataaa caaactcggg ctactataac cggcctcaaa
     5341 cgcaatatcc gtcacggaat agttggtcat ttccagttgt tttttggcaa aattaatacg
     5401 gatctcatta ataatctgca ttggtgtttt gccataatat cgttgagtcg ctcgcgtcaa
     5461 atattcctgt gatttggctg acaacgtcac catattctcc agcgccgatt cactaaactg
     5521 ctctttatca tgcatctttt ctaccgtact tttcagccac tgcggtacat catcaatcac
     5581 ctgttcttcg cggtaatgac gtaaacggtt aatgacataa aaagtaacca tctcaacaaa
     5641 ctcttccagc cctgtttcgc ggaaattcaa tgaagaaatc actgtttcca cataggtgag
     5701 aaaagcgtta ttggtccggt atacctgcga agcgacaaag caataaggca acaacggcag
     5761 gtaatgctgc tcaaaaaagc gtttactgat cccaacgttc aatatacgcg tggcaccaaa
     5821 ctcataaaaa ctttgatggt gcgaacctaa cggaataaaa acaaaatcgc cccgttccag
     5881 taacacgcgc ttaccgttaa tctcctggaa ataacgcccg gttaatacca gagtaaattc
     5941 ataatagtcg tgctgatgca gcccgctgat actctcagtt ttgttataga taaacacatg
     6001 gaagtttttg ccattaaaca actgctgttc tcgggcagtg gcaatttccg gcgcgttaat
     6061 cactggctgc atcatcgact ccttatgcct tcagtttttc atgaagctca attaattcag
     6121 taatcagttc acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg accagcacca
     6181 gactcacttt catcttacct tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat
     6241 gcgcttcatt caatgccatc cgtgactgat ccatcatggc ttttgctgca gcaaaatcgc
     6301 cctgcttcgc ctgtttcagt gccgcatacg ccaggctgcg cgcttgtccg gagttgatga
     6361 taagccccat caccacttct tccagctctt cagcttccgt ttgcgtatcg ggaatgttat
     6421 cgagatccat catacatcgt tcctctctat tcttaccggc acgattaccc gtaccggcat
     6481 cgattaaaat ttcagggcat tagcgatatc ttcttcgctc tcttctttat caatcgcgtt
     6541 ttgtgctttg ttagccacca caacaaaggg cagataaatt aacgttgcga taccaaggtt
     6601 gaagagtgcg accagcaatg cggcgacgct accgttggtg ttaaagaagg ctcccagacc
     6661 ggttggcatg gtccacggtg caatattggt caccggagga atgatgccca tgtagtacgc
     6721 tgcgagggtg attgccgcca gaatcggttg taccagtaca aacgggataa acatcaccgg
     6781 gttcatgata attggcagac caaacagaat cggttcgtta atctggaaga tgccggacgg
     6841 cagcgccagt tttgccacct gacgataatc agcacggcga gaggcgataa agatagccag
     6901 gatcaggcct aaagtcgcac cactgccccc aaggaaaata aaggagtcca gcatcggctt
     6961 ggcccagata tggaaggtct taccggctgc cagcgccgct tcgacggaac catattgctg
     7021 ataggtcgcg atattttcca gcgcccacgg cgtcataatg ccgttgtcca gtgcggtcag
     7081 cgccagcgcg ccatgaatac cgaagaacca gagcagtgga acaaagatca cataggccca
     7141 gcccaccacg ctacccaacg atgccagtgg ggttgagatg gtatccataa tgatctgatg
     7201 gaagttggtg ccccaggtat tcaacgccca ggcaataatc cccatcacgg aaagaataat
     7261 aaagccggga attaatgccg agaaggaacg cgatactgaa gcaggtacgc tgtcgggcag
     7321 tttaatgacc caattgcggc ggacaataaa ggtaaacatt tctgccacca ccaggccaat
     7381 aataatcccg gagatgatat tcgccccacc taaccagttt gcgccaaccg cataggcctc
     7441 accgacacta tatggggtga cggtcataaa tgctgcaacg gataacaacc cagccgccag
     7501 cgcatcgact ttacgctctt ctgccagcgc catgccaata aagaacggtg ccattaaaga
     7561 cattatccct aatgttccgt tatatacgtt gccgccaata cctttcagac cattaagtgt
     7621 ttcaatggtt gaggcgtcga ggcgaatacc taaggaataa aaaaacgacc cctccccaaa
     7681 gcttagaaaa acgttgttaa ttaatacaaa catggccccc gcaagggtta acggcattaa
     7741 gcgaataaag ccatttttga ttgcattaac gtgtggctgc tttcctattt taactgcaaa
     7801 agggaggagt accttttcaa gcgatgcaat aacattactc atagaaaaat acccttaaaa
     7861 accgcaattt aaatattgcg gtattgattt atgaaataac tctttgacgg gaaaatttaa
     7921 aataaattaa tttgctgcgg cttttttaat cgctgcaacc gcagccttaa gcacgcctaa
     7981 accatcgact ttgccataaa gcagcgagtc aattacttca accggtttgt tgggtaacaa
     8041 acgctggatt tcgggcaaca tataagcaat ctgcggccct aataacacga catcggcatt
     8101 ctgacctttt tcaccagcca gtgtttccgg aaatgcttca ataatgaccg gaacttcata
     8161 tttttctgcc tgtgcgcgca tttttgatac cagtaaagag gtagacatgc ccgcagaaca
     8221 aaacagataa atgtgtttct tttccataaa actgccctca tcgacgatta tctgtcagcc
     8281 agacactccg caagccttaa cctgcttcca tgctctgggt aacttgcgaa accaacatga
     8341 ctttttttgt ggctgaaagg agtatacggt atagacctga gcaatagtat ttctttgacc
     8401 ttataaattg tttctccttg acctgtggtt atgaccctct tcacatttcg ggcaaataat
     8461 tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc gcaatggccg gtttccgttg
     8521 ttactcaagc tttcagacga attgattact tcgcagcctg tggatcagtg tcgtattcag
     8581 cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg acaaagtagg
     8641 tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtaccg cgagcatgga
     8701 tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc cggctcatgc
     8761 cttttttgac gtctttcacc acaggctgta caaactggtc tttagtacga tcataagccg
     8821 tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc atattcttgt
     8881 tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca ttgggcgctt
     8941 tttcaagccc gtgaacgaaa cagctccgct ttcagaggat tcctgtatga cgttttaacc
     9001 accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt aaggaagttt
     9061 tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa aggcgctggg
     9121 cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtgtcgatt ttctgaaaag
     9181 ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga
     9241 ctccacgctt gccggaaagc tgtgccagat ggcgattaat gagctgcgcc aggaaactgg
     9301 caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ccgacgaaca
     9361 agattgccag gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa
     9421 gggcgcggta ttggctagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt
     9481 tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat
     9541 gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac
     9601 taaatatggt gacggcggta cggatattaa tccgctgtat cgtctcaaca aacgccaggg
     9661 taaacagttg ctgacggcat taggttgccc ggaacacctt tataagaaag ccccaacggc
     9721 cgatctggaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga
     9781 caatatcgac gactatctgg aagggaaaaa cgtacctgaa caggtcgcca gaacaataga
     9841 gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaccgttt tcgatgattt
     9901 ctggaaaaag taataatttg cacctattgg attgtgcgaa aaagagtaat ttgttcacgc
     9961 cggatgcggc gtgaacgcct tattcgacct acaaaactat gcaaattcaa tacattgcag
    10021 gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt catcaacccg
    10081 cctctttttt aatcgcctcc cgcctgttac actggataga taaccagcat tcggagtcaa
    10141 cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca atttatgaat
    10201 atcccgaaca tttacgttca ttccttaatg acttacccac ccgaccaggg gtgtatctgt
    10261 ttcatggcga aagtgacacc atgccgctct atatcggcaa aagcgttaat atccgcagtc
    10321 gggtgctttc tcatttacgc accccggatg aagccgccat gctacggcaa tcccggcgaa
    10381 ttagctggat ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca
    10441 aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc
    10501 aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt tcacgagccc
    10561 ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg cagagcatcg
    10621 ctgatgaaca aaaactttgt tatggcctgt tgggactgga accgttaagt cgcggtcgtg
    10681 catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg
    10741 aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgggtggtg tgttggcctt
    10801 ggcaaggtgc ggtggcactg aaagaacagc acccggaaat gactcaatat catattattc
    10861 aaaactggct gtggctgggg gcggttaatt cgctggaaga agcgacaacg ttaattcgga
    10921 cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg ctttccggta
    10981 actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga tttcactgaa
    11041 caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga accagcacgc
    11101 gccctggtcg gtagtgagca atttatcgcc cagttgccac gccccgttaa tcacaaacac
    11161 caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt
    11221 acagacatcc aggcgtgtca tgatgttgaa atccatcgac atctgccctg ccgtcagttt
    11281 cgcttttacc acctggtccg ctgcaaaggc aaaaggctgt aacggtttta aggtatggtt
    11341 aaagcggtct gcgctttcaa ggaacatctc gccgccttcc agcaacgtca ctatcctttc
    11401 catgccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc tggcacgcca
    11461 gtgaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac cggcagcgtt
    11521 tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg ctcccggccc
    11581 gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact cgggtgattt
    11641 acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga aaaccgccga
    11701 tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa gttttttggc
    11761 tgaaagatta ttcagcagtt gcaggcattt taccttttgc cgctggacgt tctgtcagac
    11821 gcttctcaaa attagcatta aattgctttt tctgttccgg cgtcaggatg ttgtaaattt
    11881 tgttctgggt ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc atttttgcga
    11941 tctgcgcttc agcttttgct ttatcgaagg tatcgctggc aatgatgtca tgcattgcgc
    12001 ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg gcctttcatg atttcgcgga
    12061 tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc tgatgcggac
    12121 cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg gtagtggtgt
    12181 ctgcggcatg ggccaggtta gccgcgccaa gagccagggt agaggcaaca aacagtgcag
    12241 ttaatttacg catattctat atccttcctt tcagttattt attacggctt tcttaagtag
    12301 cgtgccgtgt tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt
    12361 aaaacaatga aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg
    12421 atggagagtg atgagagaat aatacaacac gatgattttg cagagattat gaagaactat
    12481 accggatgac tggtgataaa taaagcaaat aaccaggatt aatctgtatt aatttataag
    12541 aaagcaactt aatacccgca gaatgatttc tgcgggtaag tattagctta ttttttcgag
    12601 cattaatccc gcgcgtaatc ccaacgctac caacggatta gggaataaca catactctac
    12661 atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa
    12721 cggcataaaa ttcagcgtgt cacttgccat atgcatttcg aaggacggcg agtggcgagt
    12781 aatttgcgaa accacccgat aacggagcgg cggtgttctc acgataccga cactctcacc
    12841 agatagcagc gcagcaattg cgctggcagt tactgcaaac tggcgaagat cgttttgccc
    12901 aaagggcaac gctttgccaa gttccagcgt acaggccagc gcgccaaaat gtctggcgct
    12961 gaaatgggta aacgtaccac caggttcctg atggaacacc agcgcctcca gccccgccgc
    13021 acccagccac gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta atacaccgaa
    13081 ctgcggatgc aaggagccac gaattgcggt atgtagatca aggtgccagc gcacagattc
    13141 tttgcccagg tcataaaaat cttccaggca ctgttccagt tcgcgcgccc gacaggtttc
    13201 tccgctttca gcaaatagct gccaacgacc gccaaacatt cgattcatat cgctatggca
    13261 ataacgtttc ccttgcttca gcgcaggagg attcccgagg atcaccagca accgccaacg
    13321 taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg tccagcatct ccacaggtgc
    13381 cgtctcatta ccgtgtattc ccgctgaaat caccagtgcg ccttgcggtg gcgttaatgg
    13441 cgtcagttcc agcacaccat cgcccagcca gcgccagcga acgccgttga tttctcgctc
    13501 ggtgataacc ggttttttac ccgttaaggt cagagcaaga aaattatcca ttgccccctc
    13561 cctcgcgctg gaacggataa accgaaccga gattcagtaa ttggctcaat acatccagtg
    13621 cttcccgccc tccgcgcagc aattgcgggt cggccagatc ggcagcagta aggcgatcgc
    13681 ggtagtaacg atccacccag tcattgagcg cattaaacag cgtatcgttc atcatcaccg
    13741 ccggattcac cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc aggcacgccg
    13801 ggccgccgcc attcgccatg ctttcacgta aatcaaagac ttttagttcg ctaatcgggt
    13861 tgtcagcggc aaggagttca ttgagataac cccatactcc ggcgtgttcc cgacactcct
    13921 gaggcagcac caacatcatg gaaccatcat cgcggctcaa cagttggctg ttaaacagat
    13981 aggtagacac cgcatccgac acggaaacct gagttgccgg aacttctatc gccataaaac
    14041 cgttcacccg cgcacgcagg tttgccagta actgtgactg gcgagcgaac gcctgttggt
    14101 ggcaaaacag cacctggcgg ttactcacgg caatcacgtc attatgaaaa acgccctggt
    14161 cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg gggattcacc tgattcagcc
    14221 ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat
    14281 cattgccttc ttctcgcccg tagacaaaaa gttgcatacc cggctcaccg taatgaccgc
    14341 cgagacgatt gtggtttgcc gccccctcat caccgagcaa cgctacctgc ggcaacgccg
    14401 aatgaacgct aaatttctct tcgtcgttaa aaatcgcttt taaaagtgat tcggtgacgt
    14461 gcgcttccag cgagcggtga aatttattgt tcaggttggc aacggtgaga tgcactttgc
    14521 catccagcgt atcggcagat ggcgcgatcg ttgccgcatt ggctacccac attggcgaag
    14581 cggaactgac gctggaaagc cagtgcggtg cctggcgtgc aactttttcc agtacctgct
    14641 catcgctacc actgaatccc aactgacgca gcaccggaat aaacggacgc tcgtgcggcg
    14701 ggatcacggc ctgggggaat cccgcatcgg caagggcttt cattttcagt aagccctgtt
    14761 ttgccgcctg tcgcgggtta gacacctgaa aacggtgacg ggtagaggct tcattaccaa
    14821 acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg
    14881 cgttcatcgc accacctcat cggaaaaatc cagcccgggg ttaagcgttg cgggcaaagt
    14941 taacgagtcc gactccaggc tcgccatagg ccatgcgcag taatctgcgg cataccaggc
    15001 gctggggcga tggttaccgg atgcaccaat gccgccgaat ggcgcggtac tggcagcacc
    15061 agtaagcggt ttgttccagt taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa
    15121 tttttcccgc tcgggggaaa ccagaccgca agagagtccg aagcgagtgt tattcgccat
    15181 tagaatcgct tcctcgaaag aatcataacg ccagacgcgc aataacggtc cgaacacctc
    15241 ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc cccggcgtca gcaacgatgt
    15301 ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg
    15361 ccaggcagta accacctgct gtgcggcctg ttcagaaatc agcccgccaa taaacggctg
    15421 cggttcgtca tcccagttgc ccggcgttaa tcgctggctg acggcaacca gacgagcaag
    15481 aaacgcatcg ccctgcgctc cgcttttgag caataaacgg cgggcgcagg tgcagcgttg
    15541 cccggctgtg acaaacgccg actgaatggt cagatggaca gccgcgtcga tatccgccac
    15601 ctcatcgata attagcggat tattaccgcc catctcaagg gcgagaattt tctccggctg
    15661 accggagagc tggcgatgca gctggtagcc ggtattggcg ctaccggtaa acagcaaacc
    15721 gtcgagatcc tccagcgcac tcagcgcctg acccgtttca cgcccgccct gcaccaggtt
    15781 cagtacgccc ggcggcaagc cagcctgctg ccataaacgc attaccgctt cgccactcca
    15841 cggtgtcagt tcgctgggtt taaagataat ggtgttacct gccagcaatg ccggaacgat
    15901 atgtccgttc ggcaaatgac cagggaaatt atacggccca aacaccgcca gcacgccgtg
    15961 cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct cgccggtacg
    16021 aacgtgatac gccttaattg atatcgcgat tttattgatc atcgccgtca cttcggttgc
    16081 cgcttcccag cgcggcttac ccgtttctct ggcaataatc gcggttaatt cgcctttatt
    16141 cctttccagc aatccggcaa agcgttcgac aacgacctga cgttcagcca atgagagccg
    16201 cgcccagcgc ggaaacgccg cacgggctgc gcgacaagcc tgcccgacct gagcggcatc
    16261 ggcatcattg ccttgccata acacctcgcc cgataccgga ttacgcttca cacgcgatgc
    16321 gccctggccc gttatccagt caccgttaat ccataaagtc atgctgtttt ctcctctgcg
    16381 cacaggcgca ccagacgaac gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg
    16441 gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg
    16501 tgataatttt cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct
    16561 gccacttcca ccagccgact tttacgaatg gcgcgcacgc ggtcgatgtc acactcaagc
    16621 gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc tttctccagc
    16681 accgcgcggg caggcgcggt ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg
    16741 gataaaaagt gggtatagat cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc
    16801 ccggtgccgc agagaaaatc ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg
    16861 ctttgccaga acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact
    16921 ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa agcgcgattt cgacagcaaa
    16981 tagccgttgc cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg
    17041 ccggtgtgat cgttactgag aaacagcgtc ggcaatgcgt tatagacatt cagctctttt
    17101 gaggcgtgaa ccaacgtgcc gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc
    17161 acctcaatgg cacaaatccc cgccacggtg cctgtctcgc tatcttccag cacgaacaca
    17221 tagccctgct cacttttggg cagttcgcct tgccaggttt tgattgccct ttcgatacgc
    17281 gccgaaagcg tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca
    17341 agctgcatca gcgccgagac atctgatcgc tcaacgggac ggatgaccat catgatgaac
    17401 ctcggctaac aaagtgttcg caagcagctg caaagcgatc cagtccggtc gtcacctctt
    17461 cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt gccacccgca atcagtacca
    17521 tcacgcctgc tttcgccgct tcctgagaga tctgtttcgc ttgcccggcg taatcggcat
    17581 tcagtacaca gccaatcagc aaacctaagc cgcgaacttc actgaacaaa ccatagcggt
    17641 gattaatagt attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca
    17701 tctctggtgt gttgatgagc tccagcactt tgcctgccac cgccgaggcc agcggattac
    17761 cgccataggt ggtgccatga gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca
    17821 acaacgcacc gaccgggaaa ccgccgccca gcgctttggc ggtagttaac agatcaggcg
    17881 tcacgccgta gtgcatatag gcatacagtt ccccggtgcg cccgacgccg gtttgtactt
    17941 catcaaaaat caacagcgca ttgtggcggt tacacaattc acgcagacct tgtaaaaacg
    18001 cgttgctggc tggcaccaca ccgccttccc cctggatggg ttcgacaatc accgcacagg
    18061 tagagtcgtc aatcagcgcg ctggcagagt taatatcgtt atatgcagca tgacgaatat
    18121 ccgccggcag tggcgcaaaa tcctgtgaat aggctggctg cccacccgca ctgacagtaa
    18181 acagcgtgcg accatgaaac gcatttttga acgccacgat gccgctctta tggctgccgt
    18241 agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg
    18301 agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat caattttttc gccagtcgca
    18361 gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc tgttcgttca
    18421 gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga
    18481 agtcgatata ctctttcccc tgctgatccc acaagcgcga accttcgcca cgtaccggta
    18541 taaagggtgc cggagcgtaa acaggtatca tccattcatc aaagttttca cgcgtaattg
    18601 gctgagacat agcgacctct acagtaaata attcgttatt tatatgttaa caataagtaa
    18661 tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc cagtgataaa agtgcataaa
    18721 cggcggaggc taactggaaa tcaaggagtt ataaccaaac catatgcatt taaagtgcat
    18781 ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc
    18841 agaagtaaaa tataattctg gaattgtgat cattgacgaa atttactgga aattactgcg
    18901 ccattctgac gcagcgcgca ccaaaagcgg gcattttttg cgccatcgtt gacatcatta
    18961 acaaccatcg atcaaatcac ttaacaacag gcggtaagca acgcgaaatt ctgctaccat
    19021 ccacgcactc tttatctgaa taaatggcag cgactatgaa atttgtctct tttaatatca
    19081 acggcctgcg cgccagacct caccagcttg aagccatcgt cgaaaagcac caaccggatg
    19141 tgattggcct gcaggagaca aaagttcatg acgatatgtt tccgctcgaa gaggtggcga
    19201 agctcggcta caacgtgttt tatcacgggc agaaaggcca ttatggcgtg gcgctgctga
    19261 ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc
    19321 ggcggattat tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt
    19381 acttcccgca gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt
    19441 atcagaatct gcaaaactac ctggaaaccg aactcaaacg tgataatccg gtactgatta
    19501 tgggcgatat gaatatcagc cctacagatc tggatatcgg cattggcgaa gaaaaccgta
    19561 agcgctggct gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa tggatggaca
    19621 ggctgatgag ctgggggttg gtcgatacct tccgccatgc taatccgcaa acagcagatc
    19681 gtttctcatg gtttgattac cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg
    19741 acctgctgct cgccagccaa ccgctggcag aatgttgcgt agaaaccggc atcgactatg
    19801 aaattcgcag catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct
    19861 aatttagcag ctctcctggc tcaaactggg tcaggagaat taaccttgag aaaaatcaac
    19921 aaactgtcag taatgatttg ttgcctgccg tcctttgtta taccgtcact gcgtttttag
    19981 ttgtctgacc acttctctat tatcaagttt gatataggaa actccacgat gaacgctgag
    20041 cgtaactttc tgtttgcctg tcttattttt gcgctggtca tttacgctat ccacgctttc
    20101 ggtttattcg atctgctcac cgatttaccc cacttacaga cactcattcg ccagagcgga
    20161 ttgttcggtt atagcctcta tattctgtta ttcatcattg ccaccctctt tctgttacca
    20221 ggaagcatac tggtgatcgc tggaggaata gtttttggcc cgctcttagg gacactactc
    20281 tcattaattg ccgccacgct ggcctcctcg agctcattcc tgctggcgcg ctggctggga
    20341 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    20401 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    20461 aatattcaaa attacgctta cggattaacc accatcgcct tctggcctta tacccttatt
    20521 tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc
    20581 agtgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    20641 attctcgtcc agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg
    20701 cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc
    20761 agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg
    20821 ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca
    20881 accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg
    20941 tttcctcacc gacgtcactc gtcacggtat tgtgtttaaa gatggctcca acggacataa
    21001 atcgctgttt atggcttatg cgaccccgaa agcattttat gaagccctga aagaggcagg
    21061 tggtacgccg ggcgaaaata tgacgatgga taataaagaa acgactcttg tcacaggcag
    21121 caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt
    21181 gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc
    21241 agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag
    21301 caatgcaaca tacacttatg gtgcggtgga aaaacgcggc gaagtgaaat tcaaaggcaa
    21361 tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata
    21421 aagccaggat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc
    21481 ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    21541 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    21601 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc
    21661 attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg
    21721 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    21781 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac
    21841 agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg
    21901 ccttttgtcc ctttcgatcc aatcagctat gctgccggtc tgacttcaat acgttttcgc
    21961 tcgtttttta tcgccaccgg gcttggtcaa ttaccggcga ctattgttta ttcctgggcg
    22021 ggcagcatgt taacaggcgg tactttctgg tttgtcaccg gactgtttat tctgtttgcc
    22081 ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgac
    22141 tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag gcgtggctgc
    22201 atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg atcccgttta
    22261 tcttttattt ggtgtcaatg tttgttgggt ggctggagcg caaacgctca ccgctcgatc
    22321 cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg
    22381 tggatatcac cagtatgaaa gtcgccgagc gtaccggcag caacgataaa ctgctggcag
    22441 tggctgactg gcggcaaagc ccgctcttta gcgatgaaga acggctggcg ctggagtacg
    22501 ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc cctgcgtacc cgactggctg
    22561 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt
    22621 cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
    22681 aacgttctta aggagggaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
    22741 tttctttggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
    22801 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
    22861 gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctc gcggatgccg
    22921 cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg acgtaaaacg ggcggctcgg
    22981 tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaaagaggcc aatttattac
    23041 aaacgggctg ggcggagact ctgcccaact ggcgctatgt cgacacacag ctgccggtgc
    23101 gggaagattt ttcagtgccg acacaaggtg cggaatcgcc ctggggcggc gcacaactga
    23161 cgtttatcgc ccgccgcgat gttacgccac agccaccaca aacgccgcaa gccttactgg
    23221 agtttgctaa agtcaatccc ggcacggtta cttatccgcg cccaccggac tttaccggca
    23281 cggcgtttct tgaacagttg ctgattatgc tgacgccaga tcccgccgca ttaaaagaag
    23341 cgccggacga tgcgactttc gcccgtgtca ctgctccctt gtggcaatat cttgatgcgc
    23401 tacatccgta tttgtggcgc gaaggaaagg atttcccgcc ttcgcccgcg cggatggatg
    23461 ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt tagccccgcg catgcgcagc
    23521 aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag ttttggcttt cgcgagggga
    23581 tgataggcaa cgtgcatttc gtcaccattc cagccaacgc gaatgccagt gctgcggcga
    23641 aggtagttgc caatttcctg ctctcacccg atgcgcaact gcgtaaagca gatcccgttg
    23701 tctggggcga tccttctgtt ctcgatccgc aaaaactgcc tgacgggcag cgcgaaatat
    23761 tgcaatcaag aatgccgcag gatctgccgc cggtactggc tgaaccgcac gcaggatggg
    23821 tgaatgcact ggaacaagaa tggttacgcc gttacggtac gcattaatct ttttgctgtg
    23881 ggcgatggtg gcggtgattt atgcaccgct gatcccggca gctctcacgc tgatatcgcc
    23941 tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat ccacagttac cgcatgcatt
    24001 actggcaacg ctggtgtcga caaccatcgc ggcggtcggg gcattgttga ttgccctgct
    24061 ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt atgtgcgccc gtctgccgtg
    24121 gctgctcgcc attccccatg tggcttttgc caccagcgcc ctgctgctct ttgctgacgg
    24181 agggctgcta tatgactatt tcccgtattt cactccgcca atggaccgat ttggcatcgg
    24241 tctgggcctc acccttgcag tgaaagaaag cgcatttctg ctgtggatct tagcggcagt
    24301 attgagcgaa aaacggctgt tgcagcaggt cattgtgctg gattcgctgg gctacagccg
    24361 ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg ccttcgctgg caatggcgat
    24421 gctggcgatt gttgcctggt cgctttcggt cgtggatgtg gcgattattc ttgggccagg
    24481 taatccaccg acgctggcag taattagctg gcagtggtta actcagggcg acgcggatca
    24541 acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg ttactcgccg cctacgtttt
    24601 gctgagctat ctgctatggc gcagctggcg gcgcactatt ccccgcgtag atggcattcg
    24661 caagcctgcc acacctttat taccaggcac tacgctggcg agttttttac ccttaaccgg
    24721 tgtgctgtgt gtggttctgc tggcgatcct cgcggatcag tcgacgatca atagtgaagc
    24781 actcatcaac agcctgacaa tggggctggt ggcgacattc atcgctttgc tcctgttact
    24841 gctgtggctg gaatgggggc cacagcgtcg ccagttgtgg ctctggttgc ccattctatt
    24901 gcccgctctg ccactggtgg cgggccagta cacgctggcg ctatggctga acctggatgg
    24961 aagctggacg gcggtggtct gggggcatct gctgtgggta atgccgtgga tgctgtttat
    25021 cctgcaaccc gcctggcagc gcattgattc acggttaatt ttgattgcgc aaacactggg
    25081 ctggtcgcgg gccaaaatct tcttttacgt gaaatgccca ctcatgttgc gccctgtgct
    25141 gattgccttc gcggtgggat tttcagtcag tattgcgcag tatatgccga cgctgtggct
    25201 gggcgcgggg cgttttccga cgctcaccac tgaggcggtg gcattaagca gcggcggcag
    25261 caacggtatt ctcgccgccc aggctttatg gcaactgttg ttaccgctta ttatttttgc
    25321 cctgaccgcg ttagtcgcaa aatgggtagg ttatgtcaga caaggactcc gctaatgctc
    25381 tgcgtgaaaa atgtttcgct acgtttacca gaaagccgct tgctgacaaa cgttaacttt
    25441 acggtggata aaggtgacat tgtcacgtta atggggccgt ctggctgtgg aaaatccact
    25501 ctgttttcat ggatgattgg tgcactggct ggacagtttt cttgtacagg tgagctatgg
    25561 ttcaatgagc aacggattga catgctaccc accgcacagc gtcagattgg cattcttttc
    25621 caggatgcac tgttatttga ccagttcagc gtcgggcaaa atttactgct ggcgctaccg
    25681 tcgacgctca aggggactgc caggcgcaat gccgtgaaag acgcgcttga tcgagcggga
    25741 ctggctgaaa cttatcacca ggaccctgcg acattatcag gcggtcagcg agcgcgggtg
    25801 gccctgctgc gcgcccttct cgctcagcca aaagcgttac tcctggatga gccattcagc
    25861 cgtcttgatg tggctctgcg cgataatttt cgccagtggg tgttcagcga agttcgcgaa
    25921 ctggcgatcc ccgtcgttca ggtaacgcac gatctccagg atgttcctgc tgatagttct
    25981 gttctggata tggcgcagtg gtcagaaaat tacaacaaac tgcgataacg caaagttttt
    26041 ctcaatgcgt cagttcagaa tggcgcactc aaaactacaa tgtcgggatt ttcgatgaaa
    26101 cgtgtttctc aaatgaccgc gctggcaatg gctttagggc tggcttacgc ttcttcgtgg
    26161 gccgctgaac tggcgaagcc tcttacactt gaccagcttc aacaacaaaa tggcaaagcg
    26221 atagatactc gccccagcgc gttttataac ggctggccac aaaccttaaa tggcccttct
    26281 ggtcatgaac ctgccgcctt aaacctctct gccagctggc tcgacaaaat gagcaccgaa
    26341 cagctctatg agtggatcaa gcaacataat ctgaaaaccg atgctccggt ggcgctgtac
    26401 ggcaatgaca aagatgtcga cgccgtcaaa acgcgtctgc aaaaagcagg ttttacgcat
    26461 atctccattc tgagtgacgc gctaagcgaa ccttcccgtc tacaaaaact gccgcacttt
    26521 gagcagctgg tttatccgca atggctgcac gacctgcaac aaggtaaaga ggttacggcg
    26581 aaacctgccg gtgactggaa agtcattgaa gcggcctggg gcgctcctaa gctttacctt
    26641 atcagccata ttcctggcgc tgactacatc gataccaacg aagtggaaag cgaaccgctg
    26701 tggaacaaag tttctgatga acaactaaaa gcgatgctgg caaaacacgg cattcgccat
    26761 gacaccacgg tcattctgta cgggcgtgac gtatacgctg gagcgcgtgt ggcgcaaatt
    26821 atgctctacg caggcgtgaa agatgtgcgt ctgctggatg gcggctggca aacctggtcc
    26881 gacgcgggac tgcctgttga gcgaggaacg ccaccgaaag tgaaagcgga accggatttc
    26941 ggcgtgaaga tcccggcaca accgcagttg atgctcgata tggaacaggc ccgtggactg
    27001 ctgcatcgcc aggatgcatc actggtgagc attcgttcgt ggccagaatt tatcggtacg
    27061 accagcggtt acagctatat taaaccaaaa ggtgaaatag ccggagcacg ttggggacac
    27121 gctggtagcg actcgacgca tatggaagat tttcataacc cggatggcac catgcgcagc
    27181 gccgatgata ttaccgctat gtggaaagcg tggaatatca aaccagagca gcaagtttca
    27241 ttctactgcg gcaccggctg gcgcgcgtcc gaaaccttta tgtacgcacg cgccatgggt
    27301 tggaagaatg tttccgtgta tgacggcggc tggtacgaat ggagcagcga tccaaaaaat
    27361 ccggtagcaa ccggtgaacg cggcccggac agtagcaaat aacattgcaa ttactgacgc
    27421 tggagcgact tcagcgtcag atacccactc cacacccgcg taaaggtcgt catccagcac
    27481 aacgcaccga agatccacgc aaaccacgca aaccatgcag ggaataaaca tcccagcaca
    27541 aacagtaaga tcgtttctgt gccttcggtt aatcccccca gatagtaaaa cgacttatgt
    27601 gcataaccgg ggttatcaat ctgatgtttt gctgccagtg cagcaaaggc gagaaaactg
    27661 ctgcccgtgc cgataaacgc aaacaacaac cagccgcctg ccagcgcatt ttgctccggt
    27721 gcagcaagaa taaagccaaa cggcaccagc gcgtaaaaga gaaaatcgag agaaatatca
    27781 agaaagccgc ccgcgtcggt aagctctctg cgccgcgcca gcgcaccgtc cagaccatca
    27841 agcagcctgt tcaacaaaat gacgactagc gccgccagat accagcccag cgccagaaac
    27901 ggcagagcca gcacgccgat ggcaaatccc actaatgtta aaccatctgg cgtaatgccc
    27961 ggtttatcaa gaacccgcac gcactgatgc aacaacggtt taatccgggg atgaagatgg
    28021 cggtctagca caggcactcc ttaaatataa agcctttctg attgagcaac agtgcggata
    28081 ttatggcatt tttcgcttat ctgcccgtgt gtaatttatg aaaatgattg aagttgttgc
    28141 cgccatcatt gaacgtgatg gcaaaatttt actcgcgcaa cgccccgccc agagcgatca
    28201 ggcgggatta tgggagtttg ccggtggtaa agtcgagctg gatgaaagcc agcagcaggc
    28261 actggtgcgt gagttaaacg aagaattggg catcgaagca actgtgggtg agtatgttgc
    28321 cagccatcag cgagaagttt cgggccggat tatccatctt catgcctggc acgtacccga
    28381 cttccacggg acgttacagg cacatgaaca tcaggcgctg gtctggtgct cacctgaaga
    28441 ggcgctgcaa tatccgctgg cccctgctga cattccacta ttagaggcgt ttatggcttt
    28501 acgcgccgcc agagcagcgg attagtgcta agggttttgt catcccgctg gcattgcagc
    28561 agtattcctt cggctttaat taccgcccct tcagaataat tttgatcctg ataaacgcag
    28621 cactgagtac agggctgcgc tgactgcccg cctgaactga acacttctgg cggtacgttt
    28681 acctccacgt ccggacgata atgcgggtta gccagtgcaa ttaatggaaa tgctaatact
    28741 acggcgaaca atgctcgact cacagggaac tccttaacgt tattgtctct gctactgata
    28801 acggtagccg ggtggcaaaa ctttagcgtc tgagttatcg catttggtta tgagattact
    28861 ctcgttatta atttgctttc ctgggtcatt tttttcttgc ttaccgtcac attcttgatg
    28921 gtatagtcga aaactgcaaa agcacatcac ataaacaaca taagcacaat cgtattaata
    28981 tataagggtt ttatatctat ggatcagaca tattctctgg agtcattcct caaccatgtc
    29041 caaaagcgcg acccgaatca aaccgagttc gcgcaagccg ttcgtgaagt aatgaccaca
    29101 ctctggcctt ttcttgaaca aaatccaaaa tatcgccaga tgtcattact ggagcgtctg
    29161 gttgaaccgg agcgcgtgat ccagtttcgc gtggtatggg ttgatgatcg caatcaggta
    29221 caggtcaacc gtgcatggcg tgtgcagttc agctctgcca tcggcccgta caaaggcggt
    29281 atgcgcttcc atccgtcagt taacctctcc attctcaaat tcctcggctt cgaacaaacc
    29341 ttcaaaaatg ccctgactac cctgccgatg ggcggtggta aaggcggtag cgattttgac
    29401 ccgaaaggta aaagcgaagg cgaagtgatg cgtttctgcc aggcgctgat gaccgagctt
    29461 tatcgtcatc tgggcgcgga taccgacgtt ccggcaggtg atatcggcgt tggtggtcgt
    29521 gaagtcggct ttatggcggg gatgatgaaa aagctctcca acaataccgc ctgcgtcttc
    29581 accggtaagg gcctttcatt tggcggtagt cttattcgcc cggaagctac cggctacggt
    29641 ctggtttatt tcacagaagc aatgttaaaa cgccacggta tgggttttga agggatgcgc
    29701 gtttccgttt ctggctccgg caacgtcgcc cagtacgcta tcgaaaaagc gatggaattt
    29761 ggtgctcgtg tgatcactgc gtcagactcc agcggcactg tagttgatga aagcggattc
    29821 acgaaagaga aactggcacg tcttatcgaa atcaaagcca gccgcgatgg tcgagtggca
    29881 gattacgcca aagaatttgg tctggtctat ctcgaaggcc aacagccgtg gtctgtaccg
    29941 gttgatatcg ccctgccttg cgccacccag aacgaactgg atgttgacgc cgcgcatcag
    30001 cttatcgcca atggcgttaa agccgtcgct gaaggggcaa atatgccgac caccatcgaa
    30061 gcgactgaac tgttccagca ggcaggcgta ctgtttgcac cgggtaaagc ggctaatgct
    30121 ggcggtgtag caacgtcggg cctggaaatg gcacaaaacg ctgcgcgcct gggctggaaa
    30181 gccgagaaag ttgacgcacg tttgcatcac atcatgctgg atatccacca tgcctgtgtt
    30241 gagcatggtg gtgaaggtga gcaaaccaac tacgtgcagg gcgcgaacat tgccggtttt
    30301 gtgaaggttg ccgatgcgat gctggcgcag ggtgtgattt aagttgtaaa tgcctgatgg
    30361 cgctacgctt atcaggcata caaatgggca caattcattg cagttacgct ctaatgtagg
    30421 ccgggcaagc gcagcgcccc cggcaaaatt tcaggcgttt atgagtattt aacggatgat
    30481 gctccccagg gaacatttct tatgggccaa cggcatttct tactgtagtg ctcccaaaac
    30541 tgcttgtcgt aacgataaca cgcttcaagt tcagcatccg ttaactttct gcgatagcag
    30601 cagatatgcc tgtaaagaaa tcccattaga ctattttttt gataatcttc ttcgctttcg
    30661 aacaactcgt gcgcctttcg agaagcaagc attatataat gccaggccag ttcttctcca
    30721 attgtcccgt tttgaaaagc tgtgcttgat atcgagatca tccatgataa ttccgccgcc
    30781 catattagct tcgccgagga tttaccggag cgattattag cgcaatcaga gatatagtct
    30841 gagggaaaaa cagcaaattt attcaacaag gcgataacct gctctggggc ttcctccatg
    30901 tttgctttaa aggtattggc tccatggtcg ccagaaagaa aatgctccat taaggcacaa
    30961 taactttcgc tatcttcgat accccattga tcctctaaag actcgcgtct tttacttatg
    31021 atatcgatcg agtcaaaagg aagcacatga tattggaagg tatctttgcc aggttcaggc
    31081 tttcgcggcc agaactccag cgtttcagac cattgcttat gatagaatcg ataaggtgcg
    31141 atcaattgta gcgcctgtaa cttctcgata ctgagcggct caataccttt agcctgataa
    31201 taatgcagtt gttctttttt tgctttaaaa ccggcccgaa caataagccc catcataatt
    31261 aatagataaa gaaaagagca tcccgcagta atcaggcctc tttcattcaa accgttggat
    31321 gttatcgctg cgaatacaaa cattacagc
//
LOCUS       DAZFYC010000050        30770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFYC010000050 DAZFYC010000000
VERSION     DAZFYC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.050"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1274)
                     /gene="rimO"
                     /locus_tag="ACHKW5_002526"
     CDS             complement(<1..1274)
                     /gene="rimO"
                     /locus_tag="ACHKW5_002526"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO7511899.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLREARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILR"
     gene            1487..1870
                     /gene="bssR"
                     /locus_tag="ACHKW5_002527"
     CDS             1487..1870
                     /gene="bssR"
                     /locus_tag="ACHKW5_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO7511900.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            1981..3096
                     /gene="yliI"
                     /locus_tag="ACHKW5_002528"
     CDS             1981..3096
                     /gene="yliI"
                     /locus_tag="ACHKW5_002528"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO7511901.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQVGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGIAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRSGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3093..3719)
                     /gene="gstB"
                     /locus_tag="ACHKW5_002529"
     CDS             complement(3093..3719)
                     /gene="gstB"
                     /locus_tag="ACHKW5_002529"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO7511902.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            3966..5168
                     /gene="dacC"
                     /locus_tag="ACHKW5_002530"
     CDS             3966..5168
                     /gene="dacC"
                     /locus_tag="ACHKW5_002530"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7511903.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(5215..5973)
                     /gene="deoR"
                     /locus_tag="ACHKW5_002531"
     CDS             complement(5215..5973)
                     /gene="deoR"
                     /locus_tag="ACHKW5_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO7511904.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6031..6627)
                     /gene="ybjG"
                     /locus_tag="ACHKW5_002532"
     CDS             complement(6031..6627)
                     /gene="ybjG"
                     /locus_tag="ACHKW5_002532"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7511905.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            6912..8144
                     /gene="mdfA"
                     /locus_tag="ACHKW5_002533"
     CDS             6912..8144
                     /gene="mdfA"
                     /locus_tag="ACHKW5_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO7511906.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(8185..8469)
                     /gene="ybjH"
                     /locus_tag="ACHKW5_002534"
     CDS             complement(8185..8469)
                     /gene="ybjH"
                     /locus_tag="ACHKW5_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO7511907.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(8555..9370)
                     /gene="ybjI"
                     /locus_tag="ACHKW5_002535"
     CDS             complement(8555..9370)
                     /gene="ybjI"
                     /locus_tag="ACHKW5_002535"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO7511908.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(9370..10578)
                     /gene="ybjJ"
                     /locus_tag="ACHKW5_002536"
     CDS             complement(9370..10578)
                     /gene="ybjJ"
                     /locus_tag="ACHKW5_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7511909.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            10662..11198
                     /gene="rcdA"
                     /locus_tag="ACHKW5_002537"
     CDS             10662..11198
                     /gene="rcdA"
                     /locus_tag="ACHKW5_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO7511910.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(11373..13058)
                     /gene="ybjL"
                     /locus_tag="ACHKW5_002538"
     CDS             complement(11373..13058)
                     /gene="ybjL"
                     /locus_tag="ACHKW5_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO7511911.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            13328..13705
                     /gene="ybjM"
                     /locus_tag="ACHKW5_002539"
     CDS             13328..13705
                     /gene="ybjM"
                     /locus_tag="ACHKW5_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO7511912.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(13735..13992)
                     /gene="grxA"
                     /locus_tag="ACHKW5_002540"
     CDS             complement(13735..13992)
                     /gene="grxA"
                     /locus_tag="ACHKW5_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO7511913.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            14152..14439
                     /gene="ybjC"
                     /locus_tag="ACHKW5_002541"
     CDS             14152..14439
                     /gene="ybjC"
                     /locus_tag="ACHKW5_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjC family protein"
                     /protein_id="HFO7511914.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDTLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14423..15145
                     /gene="nfsA"
                     /locus_tag="ACHKW5_002542"
     CDS             14423..15145
                     /gene="nfsA"
                     /locus_tag="ACHKW5_002542"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO7511915.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRQ
                     TIIKESRPFILDYLHKQGWATR"
     gene            15206..16108
                     /gene="rimK"
                     /locus_tag="ACHKW5_002543"
     CDS             15206..16108
                     /gene="rimK"
                     /locus_tag="ACHKW5_002543"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO7511916.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            16196..16672
                     /gene="ybjN"
                     /locus_tag="ACHKW5_002544"
     CDS             16196..16672
                     /gene="ybjN"
                     /locus_tag="ACHKW5_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO7511917.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            17024..18136
                     /gene="potF"
                     /locus_tag="ACHKW5_002545"
     CDS             17024..18136
                     /gene="potF"
                     /locus_tag="ACHKW5_002545"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO7511918.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKKKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            18231..19364
                     /gene="potG"
                     /locus_tag="ACHKW5_002546"
     CDS             18231..19364
                     /gene="potG"
                     /locus_tag="ACHKW5_002546"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO7511919.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLSKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            19374..20327
                     /gene="potH"
                     /locus_tag="ACHKW5_002547"
     CDS             19374..20327
                     /gene="potH"
                     /locus_tag="ACHKW5_002547"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO7511920.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            20324..21169
                     /gene="potI"
                     /locus_tag="ACHKW5_002548"
     CDS             20324..21169
                     /gene="potI"
                     /locus_tag="ACHKW5_002548"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO7511921.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            21229..21717
                     /gene="ybjO"
                     /locus_tag="ACHKW5_002549"
     CDS             21229..21717
                     /gene="ybjO"
                     /locus_tag="ACHKW5_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO7511922.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            21758..22885
                     /gene="rlmC"
                     /locus_tag="ACHKW5_002550"
     CDS             21758..22885
                     /gene="rlmC"
                     /locus_tag="ACHKW5_002550"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO7511923.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(23060..23791)
                     /gene="artJ"
                     /locus_tag="ACHKW5_002551"
     CDS             complement(23060..23791)
                     /gene="artJ"
                     /locus_tag="ACHKW5_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO7511924.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(24083..24751)
                     /gene="artM"
                     /locus_tag="ACHKW5_002552"
     CDS             complement(24083..24751)
                     /gene="artM"
                     /locus_tag="ACHKW5_002552"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO7511925.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(24751..25467)
                     /gene="artQ"
                     /locus_tag="ACHKW5_002553"
     CDS             complement(24751..25467)
                     /gene="artQ"
                     /locus_tag="ACHKW5_002553"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO7511926.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(25474..26205)
                     /gene="artI"
                     /locus_tag="ACHKW5_002554"
     CDS             complement(25474..26205)
                     /gene="artI"
                     /locus_tag="ACHKW5_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO7511927.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(26223..26951)
                     /gene="artP"
                     /locus_tag="ACHKW5_002555"
     CDS             complement(26223..26951)
                     /gene="artP"
                     /locus_tag="ACHKW5_002555"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO7511928.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(27169..27684)
                     /gene="ybjP"
                     /locus_tag="ACHKW5_002556"
     CDS             complement(27169..27684)
                     /gene="ybjP"
                     /locus_tag="ACHKW5_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7511929.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            27810..28133
                     /gene="ybjQ"
                     /locus_tag="ACHKW5_002557"
     CDS             27810..28133
                     /gene="ybjQ"
                     /locus_tag="ACHKW5_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO7511930.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            28130..28960
                     /gene="amiD"
                     /locus_tag="ACHKW5_002558"
     CDS             28130..28960
                     /gene="amiD"
                     /locus_tag="ACHKW5_002558"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO7511931.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQIDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(28957..29970)
                     /gene="ybjS"
                     /locus_tag="ACHKW5_002559"
     CDS             complement(28957..29970)
                     /gene="ybjS"
                     /locus_tag="ACHKW5_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7511932.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(30069..>30770)
                     /locus_tag="ACHKW5_002560"
     CDS             complement(30069..>30770)
                     /locus_tag="ACHKW5_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO7511933.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKQIARLAEQSTD"
BASE COUNT         7475 a         7676 c         8047 g         7572 t
ORIGIN      
        1 cgcaggatat cacccggctt aacgttggtt tcaccattga gataaaccgc gccgtcgatt
       61 tccggtgcat ctgccatgct gcgaccaatt gcgccttctt cgtccacttc gtcgataatc
      121 accagaattt cacggcccac tttctcttgc aggcgctcgg cggaaatctg ctgctgcagc
      181 tgcatgaaac ggttccagcg ttcttctttc acttcttccg gaacctggtc aggcagggca
      241 ttggcgtctg caccttcaac cgggctgtat ttaaagcagc caacgcgatc cagacgcgct
      301 tctctcagga agtcgagtag catctggaaa tcttcttctg tctcgccagg gaagccgaca
      361 ataaaggttg agcgtagggt cagttccggg cagatttcgc gccactgttt gatgcgcgcc
      421 agttggcgat ctacagaacc cggacgcttc atcagtttga gaatgcgcgg gctggcgtgc
      481 tgcaacggaa tgtccagata cggcaggatt ttgccttctg ccatcagtgg gatgacgtcg
      541 tccacatgcg gataagggta aacgtagtgc agacgtgtcc agatccccag tttcgataac
      601 tgttcgcaca ggctgaccat gctggttttt accggctcgc cgttgtggaa gccagtacga
      661 tgtttaacat caacgccata ggcggaagta tcctgcgaga tcaccagaat ctctttaacg
      721 cccgcatcta ccagacgttt cgcttcactt aacacttcgc caatcggacg gctcaccagg
      781 tcgccgcgca tagacggaat aatgcagaag gtgcagcggt gattacagcc ttctgaaatt
      841 ttcagatagg cataatgacg cggtgtcagt ttcacacctt gttctggcac caggctcagg
      901 aatgggttgt gtttcggttt tggcacgtag tgatgaacgt gctccagaac ctgctcatag
      961 ctatgaggcc cggtgatttc cagcactttc gggtggactt cgcggatctg atcttctttt
     1021 gcccccagac aaccggtcac aattaccttg ccgttttcat tcaacgcttc accaatggct
     1081 tccagtgatt cttgtaccgc gctgtcaata aagccgcagg tgttgacgat caccatgtcc
     1141 gcatcgtcat agctcggtac cacgtcataa ccttcagtgc gtagttcggt gagaatacgc
     1201 tctgaatcga caaggttttt cggacagcca agggaaacaa agccgatttt cggctgggga
     1261 gttactttgc tcatagctta aaaaatattc agttacagga aaggtcaggg cagggattct
     1321 acagagttct ggataaaatt tgtatcgcaa tctcattcgc tggcggaggc gaaggaaatg
     1381 taaattttgt taatttggcg tgaagaattg atcctggaca gcattttgct caaaaaatag
     1441 ccatactatt taattgcaac aaggctggaa agaggaggat cgaagtatgt tcgttgacag
     1501 acagcgaatc gatctgctga accggttgat cgacgcacgc gttgacctcg ccgcatacgt
     1561 gcaactgagg aaggcaaaag gatacatgtc cgtcagcgaa agcaatcatc tacgagataa
     1621 cttttttaaa ctgaatcgcg aactgcacga taaatcgctg cggttgaatc ttcatctgga
     1681 tcaggaagag tggagtgctc ttcatcatgc tgaagaagca ttagcgacag ccgcagtatg
     1741 tttgatgagt gggcaccatg attgcccgac tgttattacc gtcaacgccg ataagcttga
     1801 aaattgtctg atgagcttaa cgctgagtat ccagagcctg cagaagcacg ccatgcttga
     1861 gaaggcctga aaactaaggg ggagaaagcg tctccccctt catgtttaag tttttgtaaa
     1921 aatgaatttg ttatctcctc cactgactac gctttaagcc agagtcaatc cggaggcgtt
     1981 atgcatcgac aatccttttt ccttgtgccc cttatttgtc tttcttccgc tctctgggcg
     2041 gctcctgcaa cggtaaatgt cgaagtactg caagacaaac tcgaccatcc ctgggcactg
     2101 gcctttttac ccgataatca cggtatgtta atcactctgc gcggcggcga gttgcgtcac
     2161 tggcaagtag gaaaaggatt atctgcgccg ctttccggag ttccggacgt ttgggcgcac
     2221 gggcagggcg gcctgctgga cgtggtttta gcgcctgatt ttgctcagtc tcgccgcatc
     2281 tggttaagtt attccgaagt tggcgatgat ggcaaagccg gaactgctgt gggttatggc
     2341 cgcttaagtg atgatctctc aaaagtgacc gacttccgca ccgtcttccg ccagatgcca
     2401 aaactgtcta ccggcaacca ttttggcggg cggctggtat tcgacggtaa aggttatctt
     2461 tttattgctc tgggcgaaaa caatcagcgc ccgacggcgc aggatctgga taaattacag
     2521 ggcaaactgg tgcgtctgac cgaccagggc gaaatcccgg atgataatcc ttttataaag
     2581 gaatccggtg cgcgcgccga gatctggtct tatggcattc gtaatccgca aggaatggcg
     2641 atgaatccgt ggagtaatgc actgtggctg aatgaacatg gcccgcgcgg tggtgatgaa
     2701 attaatatcc cgcaaaaagg caaaaactac ggctggccgc tggcaacctg gggaatcaac
     2761 tattcaggct ttaagatacc ggaagcgaaa ggggagatcg tcgccgggac cgagcaacct
     2821 gttttttact ggaaagattc gcccgctgtg agcggcatag ccttctataa cagcgataaa
     2881 ttcccccagt ggcagcaaaa attatttata ggcgcgctga aagataaaga tgtcattgtg
     2941 atgagcgtca acggcgacaa agtgacagaa gatggccgta ttttaacgga cagagggcag
     3001 cgaattcgtg atgttcgctc tggacccgac ggttatttat acgttctcac cgacgagtcc
     3061 agtggggaat tacttaaagt tagcccacgc aattagctaa cgggaatcat caccactttg
     3121 cgcactgcgg ggcgttcagt gagttgctga taccagcgtt gcagatttgg acgcggtgtc
     3181 caggtcaggc caacgttaaa caaattgtag atgaacggtg cgatagcgat atcgcccaca
     3241 ccaaactcgt cgccagagaa ccattttact tttgccagtt ccgcatcgag cagggcaaac
     3301 agggcgtcgc actctttgca actggcatca atggcggcct gatcgcgctc ttcgggtggt
     3361 gttctgacta atcccatcag gatcccgcga tgagcattac tgagtgtctg gtttgcccag
     3421 tccatccatt tttccgcttc cgcacgacgt gccggtgagt cgatccacag gcgtttttgc
     3481 ccgtactgtg ccgccagata gcggacaatg gcgtttgatt cccaaagaat gagatcactt
     3541 tcgtcgtcac gcaacaacgg caccagcccg ttagggttca tcgccagaaa atcagcatcg
     3601 tgatttatcc caaactcacg gcccgcgaga atttgctcat aaggtagttc cagttcttcg
     3661 agcgtcagca atactttttt tacgttagtt gaattattcc gaccccacag cgtaatcata
     3721 ctcacccctt ttccgacagg cagccacagg ctgaacagga ctccgatggt gaagtaaagt
     3781 taacttttaa gcaacagctg gcaaaaaaat gacaccagaa gcagcgaagc ggcaggatat
     3841 tgcataaact ttaaaaactt taccaactta cggtttcttt aagtttgtgt gtgcgttatt
     3901 aatcaccgaa cttatcatac ggcgatataa cgtatttttt ttgaatggat actcgggtgg
     3961 catttatgac gcaatactcc tctctccttc gtggtcttgc agcgggttct gcatttttat
     4021 tcctttttgc cccaacggca ttcgcggcgg aacaaaccgt tgaagcgccg agcgtggatg
     4081 cgcgtgcatg gattttaatg gattacgcca gcggtaaagt gctggcagaa ggcaacgcgg
     4141 atgagaaact ggatcccgcg agcctgacta aaatcatgac cagctatgtg gttgggcagg
     4201 cgcttaaggc cgataagatt aaactcaccg atatggtgac ggtcggtaaa gatgcctggg
     4261 cgacgggaaa tccggcactg cgtggttcat cggtaatgtt cctcaaaccg ggcgatcagg
     4321 tttcggtggc agacttgaac aaaggtgtga ttatccagtc cggtaatgac gcctgtattg
     4381 cgctggccga ttacgttgcc gggagccagg agtcatttat tggtttgatg aatggttatg
     4441 ccaaaaaact gggtctgacc aacactacct tccagacggt gcacggtctg gatgcgccgg
     4501 ggcagttcag taccgcgcgc gatatggcat tgctgggtaa agcattgatc cacgatgtgc
     4561 cggaagagta cgccattcat aaagagaaag agttcacctt caacaaaatt cgtcagccta
     4621 accgtaaccg tctgctgtgg agcagcaatc tgaatgttga tggcatgaag acaggaacca
     4681 cggcaggcgc gggatataat ctggttgctt cggctaccca gggcgatatg cgtttaatct
     4741 ccgtagtgct gggggcgaaa actgaccgta tccgttttaa tgagtctgag aaattattga
     4801 cctggggttt ccgcttcttt gaaaccgtga cgccaattaa acctgatgcc acctttgtga
     4861 ctcagcgcgt ctggtttggt gataagagcg aagtgaatct tggggcaggt gaagcgggct
     4921 cagtgaccat accgcgtggg cagctgaaaa acctgaaggc gagctacacg ttaacggaac
     4981 cgcagcttac cgcaccgctg aaaaaaggtc aggttgtcgg gaccattgat ttccagctta
     5041 acggtaaatc cattgagcag cgtccgctga tcgtgatgga aaatgtggaa gagggcggat
     5101 tctttggtcg gatgtgggat ttcgtgatga tgaaattcca tcagtggttc ggcagctggt
     5161 tctcttaatc ttctgataac cggatggcgc gaaacgtcat ccggttatac gtcattaata
     5221 catcaactta atacggtgcg tctgcgcgta cttcacatac tcatcttccg ggcaacaatc
     5281 actcaccaca atatcaaagc gtttcaggtc acccatgcgc gccggacgca ccttgccaaa
     5341 tttactgtgg tcgacaacca gcacatgctt ttgcgccatc gacatggccc agtgttttac
     5401 cggcaactct tcaagattaa aacaggtagc gcctttactg acatgcacgc ctgccgcgga
     5461 ataaaaagcg atatccgggc aaaaattatt caacgtttgc tgaaaatcga tgggtttgaa
     5521 aatggcgttg ctggcgtgaa attcaccacc gcacagaaat gcgcggcaat ggggtttctc
     5581 tttcagcgcc agaaaggtat ttagcgaata acaaacggcg gtaaaaggga tttcattatc
     5641 aatcgcttca ataatccacg gcgtggtggt gccacagtca aaaaagagag tctgatcggg
     5701 ttctaccagt gtcgcagcca tttttgccgc ccggcgtttt tcttccacca ggcgggattt
     5761 ttgatcgctt aacaggtaat ggctggcact gcgcggttcc agaacaatat agccgccgag
     5821 caaaacgacg ggcgcactgt ggttgttcag atcgcgacga atcgtcatct ccgaaacccc
     5881 aagcagggcg gcggcgtctt taagatgtaa cttatcgctg cgttttaatt cttgcagcag
     5941 ctgcccgata cgctcttcgc gacgtgtttc cataatccct ctgaatagtt attgaagcga
     6001 gccgctcaat actacacttt ttagcagaga tcagtcacgc acccagcctt tgcggatcgg
     6061 taatgcaaaa cagaagcgat accacgattg cagacgttga tagagtttat gccccatcgc
     6121 ttgccagata atctgagcgc taaggcagcc aatcatacct gccagcaatc caccgagcat
     6181 atccagcggc cagtgcacgc caagataaac gcgcgaccag gcaatgacga cggccagcac
     6241 cattaaaagt gagccggacc acaggcgatg ccagcataaa aatgccagtg caaaggtgaa
     6301 aatcaccgta ccgtggtcgc ttgggaatga gtcatccgcc gcatgatgca ggaagttata
     6361 gccgatattt tcgacaaagg gtcggtcgtg cggaaaaaga tgtcccatcg tccaggacac
     6421 aaacaggctg accgccagcg cgatagcgat ttttatcacc agttgccgtt gtgctgtaag
     6481 cccccacaac caaagcaccg cggccagcag cggtaccacg gtaatcaaat ctttagcaat
     6541 aaaaatcgcc aacgagatca tccacggagc cgagtctggc gtcgcgttaa taagagagaa
     6601 tagagagaga ttcaaatttt ccagcataac ttcccgacgc aaagtgatta aaagggagcc
     6661 aatacaggca agtcgttgag aataaagtgc aggttaaact gggtaaagcg gcatcgtctt
     6721 atttccctca agcggcctgt ttacggtggg taattgtaac gggcataggt taaataaaac
     6781 ttaaagaaag cgtagctata ctcgcaataa tgtaagaatg tgcttaaccg tggtttcagc
     6841 tacaaaattc gctttctcgt tagctgtgct tttattaaac tctgcgcgat tattattggc
     6901 gaagaaattg catgcaaaat aaattagctt ccggtgccag gcttggacgt caggcgttac
     6961 ttttccctct ctgtctggtg ctttacgaat tttcaaccta tatcggcaac gatatgattc
     7021 aacccggtat gttggccgtg gtggaacaat atcaggcggg cattgattgg gttcctactt
     7081 caatgaccgc atatctggcg ggcgggatgt ttttacaatg gctcctgggg ccgctgtcgg
     7141 atcgtattgg tcgccgtccg gtgatgctgg cgggggtggt gtggtttatc atcacctgtc
     7201 tggcaatatt actggcgcaa aacattgaac aattcactct gttgcgcttc ttgcagggca
     7261 taagcctctg tttcattggc gctgtgggat acgccgcaat tcaggaatcc ttcgaagagg
     7321 cggtttgtat caagatcacc gcgctgatgg cgaacgtggc gctgattgct ccgctacttg
     7381 gtccgctggt gggcgcggcg tggatccatg tgctgccctg ggaagggatg tttgtcttgt
     7441 ttgccgcatt ggcagcgatc tcctttttcg gtctgcaaca agccatgcct gaaaccgcca
     7501 cgcgtatagg cgagaaactg tcactgaaag aactcggtcg tgactataag ctggtgctga
     7561 agaacggccg ctttgtggcg ggggcgctgg cgctgggatt cgttagcctg ccattgctgg
     7621 cgtggatcgc ccagtcgccg attatcatca ttaccggcga gcagttgagc agctatgaat
     7681 atggcttgct gcaagtgcct attttcgggg cgttaattgc gggtaacttg ctgttagcgc
     7741 gtctgacctc gcgccgcacc gtacgttcgc tgattattat gggcggctgg ccgattatta
     7801 ttggtctgtt ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga
     7861 ccgccgggtt aagtatttat gctttcggca ttggtctggc gaatgcggga ctggtgcgat
     7921 taaccctgtt tgccagcgat atgagtaaag gtacggtttc tgcggcgatg ggaatgctgc
     7981 aaatgctgat ctttaccgtc ggtattgaaa tcagcaaaca tgcctggctg aacgggggca
     8041 acggactgtt taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta
     8101 tctttttaaa agataaacag atgggaaatt ctcacgaagg gtaaaaaaat gcctgattgc
     8161 tttgtgcgat caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac
     8221 catcccttca cctacgctgg caagatggcg aacataagga tgcgggcggt aagccggagc
     8281 tggcgcagga gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac
     8341 gctgactgaa tcaggaacca caaccacttt gtatccactc ggcacatcga cggtgacact
     8401 ttgcgccatc gccacaccag taaagcccat taaaagcgcg cccaacagta gacaattttt
     8461 cataaacatt tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca
     8521 ttcaggtatt gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt
     8581 taagcacttt atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt
     8641 ttgccgctgc gacgaccgca ttgccggcat tttccattgc aaaactaaag cctgcctgac
     8701 gcagcatctc aatatcgtta ccgccatcgc caaagaccac cacttcgctg tcgtctattc
     8761 cccataattt ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat
     8821 cgatgctgcc gttgccggtg tggaccggca ccataatatc gccgatggcc tcatgtaatg
     8881 ctttttgtac ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaagatat
     8941 cctctaagtt gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tccgccaccg
     9001 ttttcatggc atcgtcatat tttttgagcg tataggcgct attttttccg caggcaataa
     9061 tttccacttc cgggcgcgtc agcaaatgtt ccacgacagt agtaaacgca tccttcgaca
     9121 gctcgccatt aaaaacatct ttgccttcgc tcactaccca gccgccgttt tcagccacaa
     9181 aggcaatttc attagcaatt tcagggaaga aagagatcaa ctgataatat tgattcccgc
     9241 tggcgactac aaagcgaatt ccttgtgctt tcatttgctg atactgagcc ataaaccgct
     9301 cacggttata ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt
     9361 taatgctcat caactattct ccatcgccgt ctgcgttttg gtatcgggtt tggcgacggc
     9421 tttcgcgaca atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa
     9481 tccataatgt tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc
     9541 ggtcgttgct accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga
     9601 aatggtcagc gggaagccca gcgaggcacc cagtccccag agtacaacag acaccccagc
     9661 gacccaggcg ctatcgacaa aaataatcag cccaataccc aacgccccca ttagcgcact
     9721 ggcccgaacc acggcaacgc gactgtaacg gtcgatgaac caaccgccag tgaagcgtcc
     9781 aacggtcatc cccagggtaa aaccggcata aatcagcgag ccggaagtgg ggctaaaacc
     9841 gtgaccgtca accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat
     9901 cgccagcacc acaacaccaa tcagcagcaa ctggatatcg cgataaaaag gtacgccttt
     9961 ttcgccatgc tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat
    10021 ggcgatatat ataggcgcga tgcctaccag cgccgccaat aaaatgtgca ccgttgccgg
    10081 aacgccaaag gccgtcagtg ccatcccgac accagcgcct gccagcgtgc ccaggctata
    10141 aaaaccgtgc atcatcggca aaaccgtttt attcatttct cgctcaacgg cggcaccttc
    10201 aacgtttatc gccacttccg cagaaccaaa acttgcccca aagacgccga gaccaacggc
    10261 aaagagcagg ggcgatgtca gccagagtgc cagacttaat atcatcatcc cgaccaatgc
    10321 gcaggacatg gtgaccagga tgacattacg tgtcccaaag cgtttcacta accacgccga
    10381 gcagagaata ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc
    10441 gatcgagaca gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa
    10501 caggcctggc aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg
    10561 tgaagaattt acggtcatag atcacgtcaa aataagaaga gggaagacaa cattagcaag
    10621 gttgtgtaca tttgtacaca attgcagaga gaggaaatga catgcgtcgc gctaacgatc
    10681 cgcaacggcg agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac
    10741 atgctgttac gcaccgcaaa attgctaccc ttgccggggt accgttggga tcgatgacct
    10801 actatttttc aggaattgat gagttgttgc tggaggcgtt cagcagtttt actgagatca
    10861 tgtcccggca atatcaggca ttttttagcg atgttagtga tgctccaggc gcatgccagg
    10921 ctatcaccga tatgatctac agctcacagg ttgcaacgcc ggataatatg gagctgatgt
    10981 accagctcta cgcgctggct agccgaaaac cgctattaaa aacggtaatg caaaactgga
    11041 tgcagcgcag tcagcaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg
    11101 atgcgtttat tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcgcgcg
    11161 aggagatttt gaggatggtt gagagggttg cagggtagta gataaatttt agataacaaa
    11221 aaacccacca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc
    11281 aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc
    11341 aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg
    11401 atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cataggtgcc cgcatagccc
    11461 agcgccggga tgttactgcg agctgtatca ctgatgatct ccattgctgg cgcgcaggtg
    11521 cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg
    11581 aacaagaaac agataaccac gggcacaaga ctgacaatca atccggcaat caacatctga
    11641 ccgccaatcg cgcccagacc gttattaata ccgctaccgg cgctcagacc aacgcctgcc
    11701 ataaacacca ttaagccgaa ctctttcacc atgcttaatg caccctgagg aatgtaaccg
    11761 aaggtcgggt ggttagcacg cataaagccc agcataattc cggcgaataa caacccggca
    11821 gcgttcccca tgccgaaact gaatgtgctg aactggaatg tgatcatccc gatcatcagc
    11881 ccaataacaa agaaggcgca gaatgccagc aagtcagtga cctggctgtg aatcgagata
    11941 aagccgatgc gatcggcgat ggtttttacg cggcgggcat cgccgctgac ttgtaaaacg
    12001 tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct acgaatgacg
    12061 cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt gccaacggcg
    12121 ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg
    12181 aaaacttctt tgccgttacg gaagctggga tcgagtcggg catgggcgtc gggatagcct
    12241 accaacgcta tttcatcgcc catttgtagc acggcatcac cgtctggatt tgccagaatc
    12301 ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc gataaatacc cagttcacgc
    12361 agatttttgc cgtcggtcca ggccaccagc tccgggccga cgcgataggc gcggatcacc
    12421 ggtaaataaa ccttacggtt ggcatcagtg tccaggccac gttcgcgggc gatttgctgg
    12481 gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa
    12541 ctcaccagac cgattaaata ggttaaggca tacccgaggc tcagattatc cagtgccagt
    12601 gagagctgcc tgctttccat gccggaatga cgcagtgtat cgccagcacc gaccagaacc
    12661 ggtgtcgacg tcatagagcc tgctaacata ccggccgtca ggccaatatc ccagccaaac
    12721 agcttaccta accctaaggc gatcaccagc gcactgccaa ccatcaccag tgctaacatt
    12781 aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg
    12841 acgcagaaaa taaacagcat aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt
    12901 tgttggccta ataacagtga tacgactaaa acgccaatgg aattacccag ttggatcgaa
    12961 ccaagtcgta actttccgag acatagccca agcgcgagga ccacaaataa taacagaatg
    13021 taattcccat ttaacaattc ggcgacgttt atattcacgg aggctaactt cttgtttact
    13081 agtaagctgt tgaaagaaat ggtaatttac gataatgttt tttaccagaa ttcagggcgc
    13141 agattcattc agcgcaccta aacgatagta aagtaacaat atattttact agtgtaatca
    13201 cattaggtat caacggctat atgaattgcg ttggcctata ttagcatgga atgtgaagcg
    13261 gctttatctt actgaacgcc acactggcga aaaatgtgtt cgatagacgc agtgtcagga
    13321 ggaacgagtg aaacataaac aacgttgggc gggggcaatc tgctgttttg tcctcttcat
    13381 tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc
    13441 tgaaatcggc ttgctatttt tcattcttcc tggagcagtc gccagcttct tttcacagcg
    13501 tagagaagtc ctgaaacctc tgtttggcgc aatgctggcg gcaccctgtt caatgcttat
    13561 tatgcggctg tttttttcac cgacccgctc attctggcaa gagctggcat ggttactaag
    13621 cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta tttatcagta gtttgtttaa
    13681 accacagcac agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc
    13741 gtccagattt tctttcaccc atgcagcaaa atcggtatag ccgccgatat gttgctgatc
    13801 gacaaaaatc tgcggcacgg tttctacggg tttacctgcc ttttgttgta gatcttcttt
    13861 agtgatccct tccgcacgaa tatctacata ctgatactga aaatcatcgc gttcattgct
    13921 caatttctca gccagatctt ttgcacgcac acagtaaggg caacccggac gaccaaaaat
    13981 aacggtttgc attatttctc tcctcataga tttatgcctg taatgatcac gctaaaatgt
    14041 attcgctgaa agtaggttta acctgttgca ttaattgcta aaagctataa ctgttaaaca
    14101 caatacagtg aaaagtttta gactgaaggc tcactttgca gagggaagcg tatgcgcgcg
    14161 atcggtaaat tgcctaaaag cgtgttgata ctggaattta tcggaatgat gttgctggcg
    14221 gtggcgctgc tgtcggtaag cgacaccctg tcgctgcctg agccattttc tcggccagaa
    14281 gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt
    14341 attcttcagg tggcaaaacg tcttgcccca cagctgatga accgtccacc gcaatattca
    14401 cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa cttatttgtg gtcatcgctc
    14461 cattcgccat ttcactgatg aacccatttc cgaagcgcag cgtgaggcga ttattaacag
    14521 cgcccgtgcg acgtccagtt ccagtttttt gcagtgcagt agcattattc gcattactga
    14581 caaagcgtta cgtgaagagc tggtgacgct gaccggcggg caaaaacacg tagcgcaagc
    14641 ggcggagttc tgggtgttct gtgccgactt taaccgccat ttacagatct gtccggatgc
    14701 tcagctcggc ctggcggaac aactgttgct cggtgtcgtt gatacggcaa tgatggcgca
    14761 gaatgcatta accgcagcgg aatcgctggg attgggtggg gtatatatcg gcggcctgcg
    14821 caataatatt gaagcggtga cgaaactgct taaattaccg cagcatgttc tgccgctgtt
    14881 tgggctgtgc cttggctggc ctgcggataa tccggatctt aagccgcgtt taccggcctc
    14941 cattttggtg catgaaaaca gctatcaacc gctggataaa ggcgcactgg cgcagtatga
    15001 cgagcaactg gcggaatatt acctcacccg tggcagcaat aatcgccggg atacctggag
    15061 cgatcatatc cgccaaacaa tcattaaaga aagccgccca tttattctgg attatttgca
    15121 caaacagggt tgggcgacgc gctaaaaccg ccacgtcgat gtatgatacg cgggcttttg
    15181 accaggtctg acagagaggt gcagggtgaa aattgccata ttgtcccggg atggaacgct
    15241 ctattcgtgt aagcggctgc gtgaagccgc tatacagcgc ggtcacctgg ttgaaattct
    15301 tgatccgctt tcttgctaca tgaacataaa tcctgcggcg tcttctattc actacaaagg
    15361 ccgcaagtta ccccattttg acgcagtgat cccgcgtatt ggcaccgcca ttacctttta
    15421 tgggacggcg gcactgcgcc agttcgagat gctggggagc tatccgctca atgagtcggt
    15481 cgccattgcc cgggcgcgtg acaaattgcg ttccatgcaa ctgctggcgc gtcagggcat
    15541 cgacctgcct gtcacgggca ttgcgcattc gccggatgat accagcgatt taatcgacat
    15601 ggtcggtggt gcgccgctgg tggtcaagtt ggttgaaggc acgcagggaa ttggcgtcgt
    15661 gctggcggag acgcgtcagg cggcggaaag cgtgattgac gctttccgcg gtctgaacgc
    15721 gcatattctg gtgcaggaat atatcaaaga ggcgcaaggg tgcgatatcc gctgtctggt
    15781 tgttggcgat gaagtggtcg ctgcgattga acggcgggcg aaagagggcg attttcgttc
    15841 caatttgcat cgtggcggcg cggcaagcgt cgccagtatc acaccacagg agcgtgaaat
    15901 cgcgataaaa gccgcgcgaa cgatggcgct ggacgttgct ggtgtggata ttctgcgtgc
    15961 taatcgcggg ccgttggtga tggaggtgaa tgcgtcgccg gggctggaag gaatagaaaa
    16021 aaccaccggt atcgacatcg cgggtaaaat gatccgctgg atcgaacgcc acgctacgac
    16081 agaatattgc ctgaaaacgg gtggttagtc gcaatcacat tactgatcat ggttttgcct
    16141 gcgctttttg cgtaagctgt gccggtcttt ttatcgaaag aggttgtaca aaattatgac
    16201 atcgctggtc gttcctggtc tggatacgct gcgtcaatgg ctcgatgacc tggggatgag
    16261 tttttttgaa tgtgataact gtcaggctct gcatctgccc catatgcaga atttcgacgg
    16321 tgtctttgat gccaaaatcg atctgatcga taacacgatc ctgttttctg ccatggcgga
    16381 agtccgacct tcagccgtat tgccgctggc ggcggattta tctgccatca atgccagttc
    16441 gctgaccgtg aaagcatttc ttgatatgca ggatgataat ctgccaaagc tggtggtttg
    16501 ccagtcttta tccgttatgc agggcgtaac ctatgagcag tttgcatggt tcgtgcgtca
    16561 gagcgaagag cagatttcga tggtcattct tgaagctaat gcccatcaac tgctgttacc
    16621 gactgacgat gaagggcaaa acaacgttac cgaaaactat tttctccact gataattcct
    16681 ttcgagcacg cagtcgctgc cgcagtgact gcgcgctgca aaattatctg ctgtttttaa
    16741 ccttttctta aagattattt cacttatctt gtgtcgattt ggctttatca catagagcaa
    16801 atacgcataa aaatttgtta aataccgttt tttaatccga gctatagtct caaaccctgg
    16861 ctaaagttat tcttgcgatg cttttatata gcgagcagtg ctggccggga gagagttctc
    16921 ttttcttaca ccgcgccgat aaaaaaatat gcacgtttat tgcatatctt tcagtgtgac
    16981 aacttttgtt cgtttgttaa cgaactttca gaaggaaaga gatatgaccg ccttaaataa
    17041 aaaatggcta tcgggtctgg ttgcgggtgc tctgatggcc gtctctgtcg gcacgctcgc
    17101 ggctgaacaa aaaacactcc acatttataa ctggtctgat tatatcgccc ctgacacggt
    17161 ggccaatttt gaaaaagaaa ccggtattaa agtcgtctac gatgttttcg actctaacga
    17221 agtactggaa ggcaaattaa tggcgggtag caccggcttt gacttggtgg ttccatctgc
    17281 cagctttctt gaacgccagc tgacagcggg agttttccag ccgctggaca aaagcaaatt
    17341 gccggagtgg aagaatctcg atccggagct gctgaagttg gtcgccaaac acgatcccga
    17401 caataaattt gctatgccct atatgtgggc gacgaccggg attggctata acgttgataa
    17461 agttaaagct gtgctgggcg aaaacgcgcc cgtcgatagc tgggacttga tcctcaagcc
    17521 tgaaaatctg gaaaaactga aaagctgcgg tgtctctttc ctggatgcgc cagaagaagt
    17581 ttttgctacc gtgttgaatt atctcggcaa agatcccaac agcactaaag cggatgatta
    17641 caccggaccg gcaaccgatc tgctgttaaa gctgcgcccg aacattcgtt atttccattc
    17701 atctcaatac attaacgacc tggcaaacgg cgatatttgc gtcgctatcg gctgggcagg
    17761 tgatgtctgg caggcgtcaa accgcgcgaa ggaagcgaag aatggagtga atgtctcgtt
    17821 ctcgattcca aaagaagggg cgatggcgtt ctttgatgta ttcgccatgc ctgcggatgc
    17881 caaaaaaaaa gacgaagcct atcagttcct gaattacctg ctgcgcccgg atgtagtagc
    17941 gcatatttcc gacaatgtgt tctatgccaa cgccaataaa gcagccacgc cgctggtgag
    18001 tgcggaagtc cgtgagaacc cgggtattta tccgcctgcg gatgttcgtg cgaagctgtt
    18061 cactctgaaa gtgcaggacc cgaaaatcga ccgtgtgcgc acccgcgcgt ggaccaaagt
    18121 gaagagcgga aaataatccg cagccttaga tgccgggcgg gcgcaccaca cccgccggca
    18181 attcgcacca tcatggtgcg cttgcacaca ttcaatgccg gagagcagcc gtgaatgacg
    18241 ctatccctcg cccgcaggcg aaaacccgta aggcgctgac gccgctatta gaaatccgca
    18301 acctcaccaa atcctacgat ggtcaacatg cggtggatga tgtcagcctg accatctaca
    18361 aaggtgaaat cttcgcgctg ctgggcgcat ccggctgtgg caagtccacg ctgctgcgta
    18421 tgctggcagg tttcgaacaa ccttctgccg gacagataat gcttgatggc gtcgatttgt
    18481 cacaggtgcc gccttacctg cgccccatca atatgatgtt tcagtcttac gcgctgtttc
    18541 cccatatgac tgtggagcag aacatcgcct ttggcctgaa acaggacaaa ctaccgaaag
    18601 cggaaattgc cacccgggtc aacgagatgc ttgggctggt gcacatgcag gagttcgcca
    18661 aacgcaaacc gcatcagctt tccggtggtc agcgacagcg tgtggccctg gcccgaagcc
    18721 tttccaaacg cccgaaacta ttactgctcg atgagccgat gggcgcgctg gataaaaagc
    18781 tgcgtgaccg gatgcagctt gaagtggtgg atattctgga gcgcgtcggt gtgacttgtg
    18841 tgatggtcac ccacgatcag gaagaggcga tgaccatggc ggggcgtatc gccattatga
    18901 atcgcgggaa atttgtccag attggcgagc cggaagagat ctacgagcat ccaactaccc
    18961 gctacagcgc tgaatttatc ggttcggtta atgtctttga aggtgtactc aaagagcgtc
    19021 aggaagatgg cctggtgctt gattcgccgg ggctggtgca tccactgaaa gtcgatgcgg
    19081 atgcttcagt ggtcgataac gtgccggtac acgtagcgct gcgcccggaa aaaatcatgc
    19141 tttgcgaaga gccgcccgcc aatggttgta acttcgcggt gggggaggtg atacacattg
    19201 cctatctcgg cgatctttcg gtgtatcacg ttcgtctgaa aagtgggcag atgattagcg
    19261 cccagctaca aaacgcccat cgtcatcgta aagggttacc gacctggggc gacgaagtgc
    19321 gtttgtgctg ggaagtggac agctgtgtgg tgctgacggt ttaaggagca aagatgaata
    19381 cacttgaacc tgctgcccag tcgaaaccgc cgggcggatt taagctgtgg ttgtcgcagc
    19441 tgcaaatgaa gcatgggcgc aaactggtca ttgcattgcc atatatctgg ttgatcttgc
    19501 tgtttctgct gccatttctg attgtcttta aaataagcct ggcggagatg gcgcgcgcta
    19561 ttccacctta taccgaactg atggagtggg ctgacgggca actgtccatc actcttaatc
    19621 tcggtaattt tctgcaactg accgacgatc cgctctattt cgatgcttat ctccagtcgc
    19681 tacaggtggc tgcgatttcg acattttgct gtttactgat cggctatccg ctggcgtggg
    19741 cggtggcgca cagtaagcct tcgacccgta atattttatt actactggtg atcctgccgt
    19801 cgtggacctc gtttctgatc cgcgtttatg cctggatggg aatattaaaa aacaacggtg
    19861 tgctgaataa ttttctgctg tggctggggg ttatagacca gccgctgacc attctgcata
    19921 ccaatctggc cgtttatatc ggcattgttt acgcttacgt gccgtttatg gtactgccga
    19981 tttataccgc gttgattcgt attgattatt cgctggtgga agcagcgctg gatctcggtg
    20041 cacgaccgct gaaaacgttc tttactgtga tcgtgccgct gactaaaggt gggattattg
    20101 ccggatcgat gctggtgttt atcccggctg tgggcgagtt tgtgatcccg gaactgctcg
    20161 gtggcccgga cagcatcatg atcgggcgcg tgctatggca agagtttttc aataaccgcg
    20221 actggccggt ggcctcggcg gtagcgatca tcatgttgct gctgctaatt gtgccgataa
    20281 tgtggttcca caaacaccag caaaaaagcg tgggagaaca cggatgaata atttaccggt
    20341 agttcgttca ccctggcgga ttgtgatttt gctgctgggc tttacctttc tctacgcgcc
    20401 aatgctgatg ctggttatct attcgtttaa cagctcgaag ctggtgacgg tgtgggccgg
    20461 ctggtcaacg cgctggtatg gtgagttatt gcgcgatgac gcgatgatga gtgcggttgg
    20521 tttaagcctg acaattgctg cctgtgcggc aacggcggcg gcgatcctcg ggactattgc
    20581 ggcggtggtg ctggtgcgct ttggcaggtt tcgcggatca aatggctttg cctttatgat
    20641 caccgcgccg ctggtgatgc cagatgtcat cacgggcttg tcgctgttgt tattattcgt
    20701 cgcgcttgct catgccattg gctggcctgc ggaccgcggt atgctcacca tctggctggc
    20761 gcatgtcact ttttgtacgg cttatgtggc ggtcgttatt tcttcaagat tgcgggaact
    20821 ggatcgctcg atagaagaag cagcgatgga tctcggtgcg acgccgctga aagtgttttt
    20881 cgtcattacg ctaccgatga tcatgcccgc gatcatttct ggctggttac tggcttttac
    20941 tttgtcgctt gatgatctgg tgatcgccag ctttgtttcc gggccgggag ccaccacgtt
    21001 accgatgctg gtcttttcca gcgtgcggat gggggtgaat ccggaaatca acgcccttgc
    21061 aacgttaatt ctcggtgcgg tcggaattgt cggatttatc gcctggtatc tgatggctcg
    21121 cgcagaaaaa cagcggatac gcgatatcca gcgtgcaaga catggctgaa gacactaaaa
    21181 tttgccaacc tggctacata atgccgcgca tgccgcggca ttgttttcat ggaagacgaa
    21241 acgttgggat tttttaagaa aacatcttca tctcatgctc gcctgaatgt gcctgcgctg
    21301 gtgcaggtgg cggcgctcgc cattattatg atccgtggcc tcgacgtgct gatgattttc
    21361 aatacgctgg gcgtgcgcgg tattggcgag ttcattcatc gcagcgtaca aacctggagt
    21421 ttaacgctgg tctttttaag cagtctggtg ctggttttca ttgagatctg gtgtgcgttt
    21481 tcactggtga aagggcgtcg ctgggcgcgc tggctatatc tgctgacaca aatcaccgcc
    21541 gcaagttact tgtgggcggc ttcgctgggg tacggttatc cggagctgtt cagcattccc
    21601 ggtgaatcaa aacgtgaaat cttccatagc ctgatgctgc agaagctgcc ggatatgctc
    21661 atcctgatgc tgctgttcgt tccctcgacc agtcggcggt tcttccagtt gcaataatgt
    21721 gtataatcgt cgcccctgat gatgtgaagg tcaatgtatg cagtgcgcac tttacgacgc
    21781 gggtcgctgt cgttcctgtc agtggataac gcagccgatt ccagagcaac tctccgctaa
    21841 aaccgccgat cttaaaaatc tgctcgccga ttttccggtt gaggaatggt gcgcgccggt
    21901 gtcaggcccg gaacaagggt ttcgtaataa agccaaaatg gtggtgagtg gtagcgttga
    21961 aaaaccactg ctcggtatgc tgcatcgcga cggcacacca gaagaccttt gtgactgccc
    22021 gctttatcct gcctcatttg cgcccgtttt tgcggcgcta aaaccgttta tcgcccgtgc
    22081 ggggctgaca ccctacaacg tggcgcgtaa gcgtggcgaa ctgaaataca ttctgctgac
    22141 tgaaagccag agcgatggcg gcatgatgct gcgctttgta ctgcgttctg aaaccaaact
    22201 ggcacaactg cgtaaggcgc tgccgtggtt acaggaacaa ctaccgcaac tgaaagttat
    22261 taccgtcaat attcagccgg tacatatggc gattatggaa ggggagacgg agatctacct
    22321 tactgaacaa caggcgctgg cagagcgttt taatgacgta ccgctgtgga tccgtccgca
    22381 aagtttcttc cagactaatc cggcggtcgc cagccagctg tacgctaccg cgcgcgactg
    22441 ggtacggcag ctgccggtta aacatatgtg ggatctgttc tgcggtgtgg gtggctttgg
    22501 tttacactgc gcgacgcctg acatgcagtt aaccgggatc gaaattgcat cagaggccat
    22561 tgcctgtgca aagcagtcag ccgctgaact gggcttaacg cgtttgcaat ttcaggcgct
    22621 ggactccact cagtttgcca ccgctcaggg ggaggtgccg gagctggtgc tggttaatcc
    22681 gccgcgccgc ggcattggta aaccgctatg tgattatctc tcaacaatgg caccgcgttt
    22741 tattatctac tctagctgta acgcccaaac catggcgaaa gatatccgcg aactgccagg
    22801 ttaccgcatc gaacgggtac agctttttga tatgttcccg cacaccgcgc actatgaagt
    22861 gctgacgctg ctggtgaagc aataaaaaag ccgcaggtgc ggcttcagat tgctgatcag
    22921 gtgcgctttg ttcatgccgg atgcggcgtg tacgccttat ccggcctaca aaatcatgct
    22981 aaatcaatac attgcagagt tgatgtgggc cggataagcg cagcgcatca ggcaatctta
    23041 cttttgtcat cagtctcaat tactgcggaa accactggtc attgattttc tgatatgtac
    23101 cgtcagcttt aattgctgcc agcgcgttat tcagtttttc cagcagagct ttgttatccg
    23161 gacgtacagc gatgcccaga ccggtgccga agtattgcgg atcggtcact ttttcagtag
    23221 caacgcccag ttgcggattg gttttcagcc attcgtttac caccgctgtg tcaccaaata
    23281 ccccatcaat acgaccattt ttcagatcga taaaggcatt ctgataactg tcataggaga
    23341 cagttttcac ttcggggtgc tgatcctgaa tatatttctg gtgcgtggtg ccgttttcca
    23401 tcccaatacg tttgcctttc agatcggcaa acgttttgta ggtatctttt ttggcaatca
    23461 cgacggctga gttttcatag tagggcgtgg taaacgatac ctgcttgcta cgctcgggcg
    23521 tgatatccat accggagatt acggcgtcat attttctgaa tttcagtgac gggatcaggc
    23581 tgtcgaacgc gtgattagta aaagtacatt ctgcctgcat ttgtttgcac aaggcttttg
    23641 ccagatcgat atcaaagccg acaatctcat tattagcacc tatagattca aagggtggat
    23701 aggtggctga aacgccaaaa ttgattttct ctgcggcaga agcaccgaaa gtaaaggaag
    23761 caagtaaagc ggcaagaact aactttttca tgatggaact cccgtctgtc aatcttatga
    23821 tttttggccg tgtctgcggc atgggataac aatgccatca agtgaattta tatgcaataa
    23881 atatgattaa ataatttaaa tgaaataaaa aaagacggat accttagtga gttgtccgtc
    23941 ttcattataa gaatttatgc actatgtagg ccggataagg cgtccacgcc gcatccggca
    24001 caggcaccgt gctgatgtct gatgcgacgc tggcgcgtct tatcagacct acaaaccccc
    24061 ccggcgaatg tacgcagcta cattaatttc gccgttcgaa tgccagtgct ttgcgctcga
    24121 tcagacgcat catcagcgtc agcaggccgt tgacgaccag gtaaataatc cctgccgcac
    24181 cgaacaccat tacatcgtag gtgcgtccgt acaacaactg gctgtatccc atcacttcca
    24241 tcagcgtaat ggtgtaagcc agagaggtac ttttgaacac cagcaccact tcgttggaat
    24301 aagaagagag cgagcgttta aaggcatacg gcagcaggat cgccagcgta tcttttttgc
    24361 tcattcccag ggcgctacag gactgccact gaccttctgg gatcgcacga attgcaccgt
    24421 aaaacagctg cgtggtatac gccgcactgt tcagcgataa cgcaatcagc gcacataacc
    24481 acggttctga caacaaatgc cacagtgccg gatactcctg caaagtcgga aactggcccg
    24541 gcccgtaata aatcaggaag atctgcacca gcagcggcgt accggtaaac agcgtgatat
    24601 aaccccgcac cagccacacc agcaccggcg ttttcagcgt caggatgatg gtaaaaatca
    24661 atgccagaat cagtgccaca atcagcgagg caacggttag cgtcaggctg gtgtgtagcc
    24721 ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc ctccgctcaa aacgtgtcgc
    24781 gcgcaggtca atgcgtttga gaatgtactg actgagcagg gtgatcacca ggtaaatcgc
    24841 cgccgccaca atgtaccagg taaatggttc ctgggtacga gtagcgatgc tttttgtttg
    24901 cagcattaaa tcattcacac taatcaaact gaccagcgcg gtatctttca gcagcaccag
    24961 ccactggtta ccgaggccag gcagcgcatg acgccacatc tgcggcatca ccagacggaa
    25021 aaagatagcc gatttcgaca gccccagcgc ctgaccagac tcccactgac ccaccggcac
    25081 cgctttcaac gcgccccgaa gcgtttgcga ggcataggcg gcatacagca gtgacagagc
    25141 gatgacacca caaaggaacg ggctaacgtc gaagttctca atgtccatct gcactgggat
    25201 ctgcacgaac ccaagattga tagtgaagcc atccgaaagc gtcagcagca gctgcgagga
    25261 gccaaaatag ataaacagca ccaccagaat ttctggcagg ccacgcagaa tggttaccag
    25321 cgctgaacct gcccacgcga caggacgcca ttttgccgac tcccataccg caaagaacat
    25381 cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg ccgacggtca tcccggcggc
    25441 gcttgctaaa ggaaaaaatt cattcatcag gaattacttc tggaaccatt tgttgtagat
    25501 ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca gtgttgagtt tctgctgcag
    25561 ctcagtgttg ccctgacgta ccgcgatgcc gaggccagtg ccgaagtaat ctttatcggt
    25621 cactttgtcg cccaccgccg ccagtttcgg gttatctttc agccactcag tgaccactgc
    25681 ggtgtcaccg aagacgccgt cgatacgccc gttttgcaga tccagttttg cgttctggta
    25741 gctgtcatac ggaacggtag tgatttccgg gtgcttatcc ataatgaatt tctggtgtgt
    25801 cgtcccgttc tgaacgccga cttttttgcc tttcagctga tcaacactgg tgtatttgcc
    25861 ttgctgaccc acaaacaggg cagagttgtc atagtacggg gtggtaaaca gtacctgctt
    25921 ttcacgctcc ggagtgatat ccatgcccgc catcactgct tctacgcgac ggaatttcag
    25981 gcttgggatc aggctgtcaa acgcctggtt agagaaagta caggttgcat caatctcttt
    26041 acacagtgcc tgtgccaggt cgacgtcaaa accaacgatc tggttgtttg catcaatcga
    26101 ttcaaacgga ggataggagg cttcggtagc aaaacgaatg gtttcggcag ctgtggcgga
    26161 aagactaaaa cctgcaatta acgcggcaat cagaactttt ttcattgttg ttatcccgaa
    26221 tcttagtgag agagatagtt tttaaatgct tcggtttgcg gctcggtaaa gcagctcgcg
    26281 tcgccttgtt ctacgatatg accattttcc atatacacca ctcggctggc ggttttacgc
    26341 gccacttcaa cttcgtgggt gacgatcacc tgggtaatat tcgtttctgc cagctcacga
    26401 atgatgctga cgatttgtgc cgtaatttcc gggtccagtg cggcggtcgg ttcatcgaac
    26461 agcagtacct gcggttccat catcaacgca cgggcaatag caacacgctg ctgctgacca
    26521 ccagaaagat gcagcgggta acgatcgcta taaggtttga gacgcagacg ttccagcagt
    26581 ttttctgcac gggccagcgc ctgatcttta ctcaacccca gtacacggca gggcgcttca
    26641 atcaggtttt gctgcacggt cagatgcggc cacaggttgt attgctgaaa caccatgcca
    26701 acgttacgac gcaaatcgcg aatcgctttg tcagagggtg ttttggtgaa atcgaaatgg
    26761 ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa gcagattgag tacacgcagc
    26821 agcgagcttt tacccgcgcc gctggggcca agtaacacca gcgtttcgcc ctgtgggcaa
    26881 tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga agcaattaat gccgtttaat
    26941 tgaatactca ttgacactcg tatactggca gtctgatagc tattgaggtc gaagatagta
    27001 cctttgacag aataattatg caatatttct gctttaaaag ttaaaagcaa agcgcattat
    27061 tcaataaaca tagcacaaaa taacgggggc ggtggtcggt gagcataaat gtcggcattc
    27121 ctcacgaaat gccggacaat ttacggggtt tattggttga tcaaggcgtt agcgattctc
    27181 gatggactga cggagcgtac ccgctgtggc atgaacgctg ccgcctaaat agcgtacatc
    27241 gtcgatcacc caacactggc cttcctgaat cattaacact tcatcctgcc agctctgatc
    27301 accctgtttt agatccacac gcagaggaat atttcgcgca tcacgattcg ggattgttga
    27361 ggcactggca acacgggcgc tctctggcaa ggttgtgcga ctggagaatg gatcgttggt
    27421 cagtagttcc cgatggttat tatcccggga ggcatcgcta agcagtgtcg ccagtttgtc
    27481 gctcagataa gggcgcaagg cggtgatgtc gttgctgcgg tgcaaaatgc ggtagtcata
    27541 aaattgctgg gccacgttat ccgggcctcc ttcaacgcaa ggaccactgc gtgtgccgtt
    27601 atctttataa gctggagtga ctgtggtgca ggcactgagg agcagtgcgc aggggataag
    27661 cattgtcaat ttgctgtagc gcataatgat ttccttataa gcgatcgctc tgaaagcgtt
    27721 ctacgataat aatgatatcc tttcaataat agcgtatcag tctgataatg ctttagagat
    27781 cgaaggctta acaaacaagg agatcgatca tgcaattttc tacaacccca actctggaag
    27841 gccagaccat cgttgaatat tgcggtgtgg tgaccggcga agcgatttta ggtgccaata
    27901 ttttccgtga tttctttgcc ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg
    27961 aaaaagaact gcgtaaagca cgggagatcg cctttgagga attaggctcc caggcgcggg
    28021 cgctgggggc cgatgccgtc gtcggtattg atatcgacta cgaaacggtc gggcaaaacg
    28081 gcagtatgct gatggttagc gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt
    28141 cttctggctg atcgctgccg ctctgttact ggcagggtgt gcaggcgaaa aaggcattgt
    28201 cgagaaagag ggatatcaga ttgatacccg acgccaggcg caggcggcgt atccgcgcat
    28261 taaagtgctg gtgatccact acaccgcaga tgattttgat agctcgctgg cgacactgac
    28321 cgataagcag gtcagctcgc attatctggt ccctgcggta ccaccgcgat acaacggtaa
    28381 accgcgcatc tggcaactgg tgccggaaca agaactggcc tggcatgcgg ggattagcgc
    28441 ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc attgagctgg aaaaccgtgg
    28501 ctggcaaaaa tcggccggag tgaaatattt tgccccgttt gaaccggcac agattcaggc
    28561 gcttattcca ctggcgaaag atattattgc ccgttatcac atcaagccgg aaaacgtagt
    28621 ggcacatgcg gatatcgcac cgcagcgcaa agacgatccg gggccattat ttccctggca
    28681 gcaactggcg cagcagggga ttggtgcctg gccggatgcg cagcgggtta acttttacct
    28741 tgccgggcgc gcgccgcaca caccggtaga taccgcgtca ttgctggagc ttttggcgcg
    28801 ctacggttat gacgttaaac ctgatatgac accgcgcgag cagcggcgcg tgattatggc
    28861 attccagatg catttccgcc cgacgttata taacggcgaa gcggatgcag aaactcaggc
    28921 gattgccgaa gcattgctgg agaaatacgg gcaggattag cgcggcagtt ttccgtggtc
    28981 gcgtagccag gcggcagttt tctcgatacc ttcatccagg gtgatgaccg gctgataacc
    29041 taactcttcc tgcgcacgcg taatatccag cgtaaagtca aaattcaact tggagacgcc
    29101 gtagtgggtc agcggcggct cttttgctga cttgcggcct aaacgctcca tgctgcgggc
    29161 gatcatatcc agcatcgggt aggggacgga acgaatacga cagtcaatat tcaactcgtc
    29221 gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg ccgttggtga tgttgtacac
    29281 acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc agccacattg cgtgcacggc
    29341 attttcatag taggtcatat cgaccagcgc actgccgcca tgcggtaaca gaatactgcc
    29401 gtagtggtgc atcatatgcg ccagacgggg aataaagact ttatcgtgcg gtccgaacag
    29461 actttgtggg cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat
    29521 cacttcttcg ctggctgctt tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa
    29581 atcttcttta atatcgcgat ggtggtgata atcgaagtac agggaggggg aagagatatg
    29641 aataaagtta cgcacacccc aggcgacagc ccattcaccc aggcggcgag tggcgcgaac
    29701 gttagccaga tcgaaagcct gttgtgtccc ccagggtgag gtaaagctgg agcagtgcca
    29761 cagcgtatca atgcccgcga gcatcacttt agcttgtgat gaaaccagct cggtcagatc
    29821 cgccggaaca aactctgcgc ccattttttc cagcaatttg cccattgcct cgttgcgacc
    29881 ggtcgctcgc acgctgatgc ctttctggca taaaaactct accgcgtttc gacctaagcc
    29941 gctggtggcg ccggtaacca gtaccttcat atcaatccac tgttgttgag aaaataacgt
    30001 gcgcattctt ccgtgatttc ccccatgatg caatgggaaa catgaaagaa taacgcaggt
    30061 tttgtcgatt aatctgtgct ttgttctgcc agtctggcga tttgttttgc cattccgcga
    30121 aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa acagccccgg cataccgtgc
    30181 ggatgccaga aagcgcggac atcgatagta cgatagtcgc ctttatcttc caggctaaaa
    30241 cacagtcgtc ccagccccgg cgctttcatg ccaaataaca acgtaagttg tttttccggt
    30301 tcaacgacaa tcactttcca gctatctacc gcatcgccag tctgtaaata ttcgcgctcc
    30361 gggcggcctt tcgccagctt atgaccgatc gcgcggtcca tcaacgcccg tgtctgccac
    30421 aaaatattgc caaagaaata acgctcttta ccgccgattt ggttcactac ctgccataaa
    30481 gcagcaaggc tggcggacgt tttaacggta aaccccgcct gtttggcaaa ataaccgtac
    30541 tccggtcgcc agcgggcaaa ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt
    30601 ttttcctcct ctttcaacgt gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg
    30661 atgagtgcac gtagcgcggt atcatccgcc agcagatcgt gtttcagccc ctgaatcaac
    30721 gccctggcgg tggtgggcgg tacggaagta atcacattga gaaaccacac
//
LOCUS       DAZFYC010000051        30735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFYC010000051 DAZFYC010000000
VERSION     DAZFYC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.051"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(292..2904)
                     /locus_tag="ACHKW5_002561"
     CDS             complement(292..2904)
                     /locus_tag="ACHKW5_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7511934.1"
                     /translation="MTNAFAWPWNAPRPAVGPYIYEPKKIAPLAGAVAHHPAVKKHID
                     HIFKRAGYNPAEVRDRDALIQALDRYEPCGLPLAAHQNIIRQEMEAAKATAAAWANTP
                     EGVEARLLSEPFFIREVWRKKIEWLRVNRETRHTNDFLMGTVKKSLLRLDVVRTRQGV
                     SPDITGELAAYWFGRWQRLADFTKREALSAANEIASRMAEMLGTECEALGQNVSDMNV
                     EELDWLYCHLGREMLALRIVPPAWYAPWERDRICTAILRMASPDWWGRKIWRLRCDWR
                     ENQLRAVGAVNKKAHAYVSASSLIEWQEQRRKNRDFFKSHELVDEDGNVSSLEDMINK
                     STSNPAIRRHELMARMAGVELVAQSRGDVGIFLTITCPSKYHSNIASGHHNAKWNHST
                     VAQAQCYLCRVWNRATAKLKREDLRPYGFRVAEPHHDGTPHWHALLFMPQEQVKATVA
                     ILRTYFIAEDRDELGRNTGARFKSKKMDPRKGSATAYIAKYISKNIDGHALAGELDDE
                     SGKPLNESAKYAMAWASLHRIRQFQPIGQPPISVYRELRKLSNQITTRQKIDSTFKRG
                     APLLVDPAMDAVCAAADVGCFATYIIRQGGVLIPRENYVVRLAYQPADEMNAYCEIPE
                     KVFGVWSPRLGDASRICTRLVKWKIRAKSKTVTGAKSGPGLGVDLLPSPTGDAWSSVN
                     NSTEDEKITDFSPGVEGITEESEEEIVDFENMDQPTRRKLIRRLREAPFKRRHSGSPY
                     EPGSELDVAWRSAVEKTEARSVADKARRAALAPAVASLLADAALCLVEISEIQAVSLL
                     MGSRLEIGGKFYQASAGGQLITRQLPNESRTVNKLWEHLRDNHGIDATHLRFDPVGEY
                     QKMLAGAEGRYPKE"
     gene            complement(2901..3791)
                     /locus_tag="ACHKW5_002562"
     CDS             complement(2901..3791)
                     /locus_tag="ACHKW5_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO7511935.1"
                     /translation="MIRSLVKWPGGKGRVMPDLLPILPKADCLVEPFVGGASVFLNTE
                     YRRYILGDINPDLINLYRQITRWPDAVIDAARPLFKVYGDKDGYKWIRDDFNARARDL
                     LSLRSVFEDGPDAGKILRAAQFLYLNRHGYNGVVRYNQQGGYNVPFGRHKTPPYFPEE
                     QIRLFSEKANDTKAIFVCCDFQSTLKIMIGSDAVIYCDPPYLPTSETANFTQYHTAPF
                     GIKEHRQLAAALLDINRLTGSPVILSNSDTPATCEIYRSFNFQEISVNRSVSANAITR
                     GAASEVIGILKVATSTGICE"
     gene            complement(3862..4245)
                     /locus_tag="ACHKW5_002563"
     CDS             complement(3862..4245)
                     /locus_tag="ACHKW5_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511936.1"
                     /translation="MDKTGQQPGRRQFLEQRARLQASLNVSRVNDTATRFNRLDDACK
                     KVIFILANDASRYIAGMPKLTAKQLGCTYENLTEKEQTCLLMGIKRLSEFAASMPWEF
                     EDYAAPRAEIQAIRDKPPAPDNAVN"
     gene            complement(4267..4488)
                     /locus_tag="ACHKW5_002564"
     CDS             complement(4267..4488)
                     /locus_tag="ACHKW5_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="HFO7511937.1"
                     /translation="MDLTQCPSLASLLTHGQQITHRQHQRGWIETPDGRFFQPKAADV
                     QFVKNCRVPFMSRPRNKRRWFSRLMGIFA"
     gene            4627..5106
                     /locus_tag="ACHKW5_002565"
     CDS             4627..5106
                     /locus_tag="ACHKW5_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511938.1"
                     /translation="MKFNVFFKKDHGSHWVLSDGSPLFESPLFETRPKAIDDLENFVG
                     LMESPIFIKAGDDINSGDTENCPSVVISLKQHESLWGWELFISKNGQLSKVTESSGNG
                     FDSLELAKQSAQFFINAIIDAPILDQADVAIPGMHFSKSFEETHHIGDIHPSSKWFK"
     gene            complement(5111..5458)
                     /locus_tag="ACHKW5_002566"
     CDS             complement(5111..5458)
                     /locus_tag="ACHKW5_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7511939.1"
                     /translation="MDTAKIFKLSDSHTALSNVFIRIKDKRTPCGNYFFRLDKLDCVS
                     LADNSGLTPLIEVTGGYVEPSVTYEQFMSFLFQVFDDVSNGDNSIRIYELMVDEETVR
                     DVPPRTRKTITPQ"
     gene            complement(5481..5756)
                     /locus_tag="ACHKW5_002567"
     CDS             complement(5481..5756)
                     /locus_tag="ACHKW5_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFO7511940.1"
                     /translation="MSKQVVSISDAVPYQEFAKLIGKTPAAVRGMIEKGKLPVVEMTD
                     PQSTSGRAGEYWVYLPAWNNGMKMAYESRPKEIREGWLMWLGLGNPS"
     gene            5880..6179
                     /locus_tag="ACHKW5_002568"
     CDS             5880..6179
                     /locus_tag="ACHKW5_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7511941.1"
                     /translation="MSTPISEKLALVRESERLNRKQFAEITGVPYSSLTYYESGRTTP
                     PTDVTMKILQHPRFSKYTLWFMTDQVAPEAGQIAPALAHFGQDVTTSQHSDQKIG"
     gene            6325..7332
                     /locus_tag="ACHKW5_002569"
     CDS             6325..7332
                     /locus_tag="ACHKW5_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7511942.1"
                     /translation="MTIKKLDDGRYEVDIRPAGRNGKRIRRKFDKKSEAVAFEKHTQY
                     NHHDKEWLAKPTDKRHLSELTKVWWDLKGKHEEHGKSNLGKIEIFTRITDDPCAFQIT
                     KALISQYSMTRRGQGIKPASINRDLTCLSGMFTALIDAELFFGEHPFRGMKKLKEQKP
                     ETGYLTQEEITLLLANLDGDNKKIAILCLSTGARWGEAAKLKAENVIQNRVTFVKTKT
                     NKPRTVPISEEVAVMIAGKGYLFPDASYPKFRRTMKDVKPDLPDGQATHALRHSFATH
                     FMINGGSIITLQRILGHSRIEQTMVYAHFAPEYLQDAVSLNPLRGGVDSQCVHTVSTV
                     G"
     gene            complement(7597..7914)
                     /gene="yegR"
                     /locus_tag="ACHKW5_002570"
     CDS             complement(7597..7914)
                     /gene="yegR"
                     /locus_tag="ACHKW5_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO7511943.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            8329..9228
                     /gene="yegS"
                     /locus_tag="ACHKW5_002571"
     CDS             8329..9228
                     /gene="yegS"
                     /locus_tag="ACHKW5_002571"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO7511944.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(9310..10083)
                     /locus_tag="ACHKW5_002572"
     CDS             complement(9310..10083)
                     /locus_tag="ACHKW5_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7511945.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(10189..11229)
                     /gene="gatD"
                     /locus_tag="ACHKW5_002573"
     CDS             complement(10189..11229)
                     /gene="gatD"
                     /locus_tag="ACHKW5_002573"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7511946.1"
                     /translation="MKSVVNDTDGIVLVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(11277..12633)
                     /locus_tag="ACHKW5_002574"
                     /pseudo
     CDS             complement(11277..12633)
                     /locus_tag="ACHKW5_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(12637..12921)
                     /gene="gatB"
                     /locus_tag="ACHKW5_002575"
     CDS             complement(12637..12921)
                     /gene="gatB"
                     /locus_tag="ACHKW5_002575"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO7511947.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARGDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(12952..13404)
                     /gene="gatA"
                     /locus_tag="ACHKW5_002576"
     CDS             complement(12952..13404)
                     /gene="gatA"
                     /locus_tag="ACHKW5_002576"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO7511948.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(13414..14676)
                     /gene="gatZ"
                     /locus_tag="ACHKW5_002577"
     CDS             complement(13414..14676)
                     /gene="gatZ"
                     /locus_tag="ACHKW5_002577"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO7511949.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(14717..15559)
                     /gene="gatY"
                     /locus_tag="ACHKW5_002578"
     CDS             complement(14717..15559)
                     /gene="gatY"
                     /locus_tag="ACHKW5_002578"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO7511950.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGC"
     gene            complement(15869..16921)
                     /gene="fbaB"
                     /locus_tag="ACHKW5_002579"
     CDS             complement(15869..16921)
                     /gene="fbaB"
                     /locus_tag="ACHKW5_002579"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO7511951.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            17178..18455
                     /gene="yegT"
                     /locus_tag="ACHKW5_002580"
     CDS             17178..18455
                     /gene="yegT"
                     /locus_tag="ACHKW5_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO7511952.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18452..19456
                     /gene="yegU"
                     /locus_tag="ACHKW5_002581"
     CDS             18452..19456
                     /gene="yegU"
                     /locus_tag="ACHKW5_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO7511953.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            19453..20418
                     /gene="yegV"
                     /locus_tag="ACHKW5_002582"
     CDS             19453..20418
                     /gene="yegV"
                     /locus_tag="ACHKW5_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO7511954.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(20392..21138)
                     /gene="yegW"
                     /locus_tag="ACHKW5_002583"
     CDS             complement(20392..21138)
                     /gene="yegW"
                     /locus_tag="ACHKW5_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7511955.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYLQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(21190..22008)
                     /gene="yegX"
                     /locus_tag="ACHKW5_002584"
     CDS             complement(21190..22008)
                     /gene="yegX"
                     /locus_tag="ACHKW5_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO7511956.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGTVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQYSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(22073..22873)
                     /gene="thiD"
                     /locus_tag="ACHKW5_002585"
     CDS             complement(22073..22873)
                     /gene="thiD"
                     /locus_tag="ACHKW5_002585"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO7511957.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(22870..23658)
                     /gene="thiM"
                     /locus_tag="ACHKW5_002586"
     CDS             complement(22870..23658)
                     /gene="thiM"
                     /locus_tag="ACHKW5_002586"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO7511958.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(23693..23789)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23881..24153)
                     /gene="rcnR"
                     /locus_tag="ACHKW5_002587"
     CDS             complement(23881..24153)
                     /gene="rcnR"
                     /locus_tag="ACHKW5_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO7511959.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            24274..25098
                     /gene="rcnA"
                     /locus_tag="ACHKW5_002588"
     CDS             24274..25098
                     /gene="rcnA"
                     /locus_tag="ACHKW5_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO7511960.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            25317..25655
                     /gene="rcnB"
                     /locus_tag="ACHKW5_002589"
     CDS             25317..25655
                     /gene="rcnB"
                     /locus_tag="ACHKW5_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO7511961.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(25737..26771)
                     /gene="yehA"
                     /locus_tag="ACHKW5_002590"
     CDS             complement(25737..26771)
                     /gene="yehA"
                     /locus_tag="ACHKW5_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO7511962.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(26785..29265)
                     /gene="yehB"
                     /locus_tag="ACHKW5_002591"
     CDS             complement(26785..29265)
                     /gene="yehB"
                     /locus_tag="ACHKW5_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO7511963.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGRSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(29281..29955)
                     /gene="yehC"
                     /locus_tag="ACHKW5_002592"
     CDS             complement(29281..29955)
                     /gene="yehC"
                     /locus_tag="ACHKW5_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7511964.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(30036..30578)
                     /gene="yehD"
                     /locus_tag="ACHKW5_002593"
     CDS             complement(30036..30578)
                     /gene="yehD"
                     /locus_tag="ACHKW5_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7511965.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         7818 a         7766 c         7084 g         8067 t
ORIGIN      
        1 aacattaatc attctatctt gaaacttgtc ttaaacaaga ttacctcttt actctgctga
       61 gtcaaacagc ctagcccact cacttgtatc actcatcttt aacatcgcag cgtagtttct
      121 aactggagca taccgaatat gaccgctatt aacattatgc cgtaactgca tatttagata
      181 ttggattagg ggcatcgcgc tgacagaact caaatgcggg ctctttgccg cccgcccccg
      241 cagaatcacg ctatccgacg caaccagctg cgacggatag cgtgatttaa ttcattcttt
      301 agggtagcga ccttccgcac ccgccagcat tttttgatat tccccgaccg gatcaaaccg
      361 caggtgcgtg gcatcaatgc cgtggttatc ccgtaaatgt tcccataatt tattcaccgt
      421 ccgtgactca ttaggtaatt gacgcgttat caactggccg ccagcactgg cttgataaaa
      481 tttcccgcca atctccagac ggctccccat taacagcgaa acggcctgaa tctctgaaat
      541 ctcaaccaaa cacaacgccg catcagccag cagactggca accgcagggg caagcgctgc
      601 ccgcctggct ttatcagcca ccgatctggc ctcggttttt tccacggcag aacgccaggc
      661 aacatctaat tcgctccctg gttcataagg tgatccgctg tgccgccttt tgaatggtgc
      721 ttcacgtagt cgccgtatca atttgcgccg cgttggctgg tccatatttt caaaatcgac
      781 gatttcctct tctgattctt ctgtaatacc ctccacacca ggcgaaaaat cggtgatttt
      841 ttcgtcttcc gtagagttat tgacagaact ccaagcgtcg ccggttggcg acggcaaaag
      901 gtcaaccccc aaaccggggc cgcttttggc cccagtgacg gttttggatt tggctcgaat
      961 tttccactta accagacggg tgcaaatacg ggaggcatcc cccagacgcg gcgaccagac
     1021 cccgaaaacc ttttcgggga tctcacaata agcattcatt tcatcagcgg gctgataggc
     1081 cagacggacg acatagtttt ctcgcgggat caaaacacca ccctggcgga tgatgtaagt
     1141 agcaaagcat ccgacatcgg cagcggcgca aaccgcatcc attgcaggat ccacaagcaa
     1201 tggcgcacca cgtttgaagg tactgtcaat tttctgtcgg gtcgtgatct gattactaag
     1261 tttgcgcagc tcgcggtaaa ccgatatggg cggctgtccg atgggctgaa actggcggat
     1321 acggtgaagc gatgcccagg ccattgcata tttggcgctt tcattcagcg gcttgccgct
     1381 ttcgtcgtcc agttcaccgg ccagcgcgtg gccgtcgata ttcttcgaaa tgtatttagc
     1441 gatgtatgcc gttgctgacc ctttccgtgg gtccattttt tttgacttga aacgagcgcc
     1501 ggtattgcgg cccagctcgt cgcggtcttc cgcaatgaag taggtgcgaa ggatcgcaac
     1561 cgtggctttg acttgctctt gtggcataaa cagcaacgcg tgccagtgtg gtgtcccatc
     1621 gtgatgcggc tcggcgacac ggaagccata agggcgcaaa tcttcgcgtt tcagtttggc
     1681 ggttgcccgg ttccacacac ggcataaata gcactgcgcc tgggcgacgg tggaatgatt
     1741 ccatttggcg ttatggtggc cggatgcaat attactgtga tatttcgacg ggcaagtgat
     1801 tgtcaggaag atgccaacat cgccgcgact ttgggcaaca agctcaaccc cagccattcg
     1861 ggccataagc tcatggcgac gaatagccgg gtttgacgtg gatttattaa tcatgtcctc
     1921 aagtgaggaa acgttgccat cttcgtctac cagttcatga cttttgaaaa aatcccggtt
     1981 tttacggcgc tgttcctgcc actcgatcag gctggaagcg ctaacgtatg cgtgcgcttt
     2041 tttatttacc gcgccaacgg ctcgcagctg gttttcgcgc cagtcacaac gcaggcgcca
     2101 gattttgcgc ccccaccagt caggcgaagc catacgcaaa atggccgtac atatccgatc
     2161 acgttcccac ggcgcatacc atgcaggcgg cacaatgcga agcgccagca tttcgcggcc
     2221 cagatgacaa taaagccagt ccagttcttc gacgttcatg tcggaaacat tctgtcccag
     2281 ggcttcgcat tccgtcccca gcatttcagc catgcggctg gcgatctcat tagcggcgct
     2341 tagtgcttcc cgctttgtga aatcagccag ccgttgccag cgcccgaacc agtacgcggc
     2401 cagttcgccg gtgatatcag gcgaaacacc ttgccttgtg cgcacaacat caagacgcag
     2461 caatgatttt ttcactgtcc ccataagaaa atcattggtg tgtctggttt cacggttgac
     2521 ccgtaaccac tcaatttttt tacgccagac ttcacgaatg aagaacggct ctgataataa
     2581 acgtgcttcg acaccttctg gcgtattggc ccaggctgcc gccgtggctt tagcggcttc
     2641 catttcttgc cggattatat tttgatgggc ggccagtggc aggccgcatg gttcgtacct
     2701 gtccagcgcc tggatgagcg catcacggtc acgaacttcg gcgggattgt aaccggcacg
     2761 tttgaagatg tgatcgatat gtttttttac ggcaggatga tgcgccaccg ccccagcaag
     2821 cggggcgatt tttttcggtt catagatgta cgggccaaca gctggacgcg gtgcgttcca
     2881 aggccatgcg aaagcgttag tcattcacat atgcccgtag aagtcgcaac cttgagaata
     2941 ccgatcacct cactggcggc ccctcgggta atggcgttcg cgctgacaga acggttaacg
     3001 ctaatttcct ggaagttgaa agagcgataa atctcgcagg tggctggggt gtcgctattg
     3061 gacaggatca ccggcgaacc agtaaggcgg ttaatatcca gcagagcggc agctaactgg
     3121 cgatgctctt taatcccaaa cggggcggtg tggtattggg tgaaattagc ggtttcgctt
     3181 gttggcaggt atggcggatc gcaatagata accgcgtcac taccaatcat gatttttaac
     3241 gtactctgga aatcgcagca cacgaaaata gctttcgtgt cgttagcttt ttcagagaat
     3301 aaacggattt gttcttccgg gaagtaaggc ggggttttgt gacgcccaaa gggaacgtta
     3361 tatccaccct gttggttgta gcgtactacg ccgttatatc cgtgacggtt caggtaaaga
     3421 aattgtgctg cacgaagaat cttgcccgcg tccggcccat cttcaaacac actgcgcaat
     3481 gacagtaggt cacgggcgcg ggcgttgaaa tcatcgcgga tccacttata gccgtcttta
     3541 tcgccgtaca ctttaaacag tgggcgagca gcgtcgatca ccgcatcagg ccagcgggtt
     3601 atctggcgat acaggttaat taaatctggg ttgatatcac ccaggatata acggcgatat
     3661 tcagtattga ggaaaacaga agcaccgccg acaaacggtt ccaccaggca atcggctttc
     3721 ggcagaatcg gcagcaaatc aggcataacg cggcctttac caccgggcca tttaacgaga
     3781 gatcgaatca tttttacttt ctccagggtg caagaagccc gacgcgttag cgcctgtttc
     3841 ttttttgtgg gtagttagtt gttaatttac tgcgttatct ggcgcgggtg gtttgtcgcg
     3901 tatcgcctga atttcggcgc gtggtgcggc gtagtcctca aattcccacg gcattgatgc
     3961 tgcaaattcg gaaaggcgct taatgcccat caaaaggcac gtttgctcct tttcggttag
     4021 attttcgtaa gtacaaccca gctgtttggc ggtcagcttc ggcattccgg ctatgtatct
     4081 ggacgcatcg tttgccagga tgaaaatcac ctttttacag gcatcatcca ggcggttaaa
     4141 acgggttgca gtgtcgttca cgcgtgagac gttcaaactt gcttgcagtc gagcccgttg
     4201 ctctaagaac tggcgacggc ctggctgttg tcctgtctta tccatcaaca taaccacctc
     4261 cccgaactac gcaaagatgc ccattaagcg ggaaaaccag cggcgcttat tacgcgggcg
     4321 cgacataaac ggtacacggc agtttttaac aaattgaaca tctgccgctt taggctggaa
     4381 gaaccgcccg tccggggttt caatccagcc gcgttgatgc tgacggtggg tgatctgctg
     4441 gccgtgggtg agcaggctgg cgagtgaagg gcactgtgtt aagtccatgt tgatctccgt
     4501 catattgaaa agatgccggg atataacccc acgcccggca cgtgtttcct gtgataacat
     4561 cgctatgcct gtgtaacttt gagcagtgaa tcaggcataa caatcaccaa cacagaagga
     4621 gttgtaatga aattcaatgt gttttttaaa aaagaccacg gatcacactg ggtactttcc
     4681 gatggttccc ccctctttga aagtccacta tttgaaacaa gacctaaagc cattgatgac
     4741 cttgagaact ttgtaggcct tatggaatcg ccaattttta ttaaggctgg tgacgatatt
     4801 aattctggcg atacagaaaa ctgtccatct gtagttattt ctttaaaaca gcatgaatcg
     4861 ctatggggct gggagttatt tatctcaaaa aatggacaac tgtctaaagt taccgaaagt
     4921 tcaggtaatg gctttgactc attagaatta gcaaagcaaa gtgcccagtt ttttattaat
     4981 gccattatcg acgctcctat tctggatcaa gccgatgtcg ctatccccgg tatgcacttc
     5041 tcaaaatctt tcgaggaaac gcaccacatt ggtgacattc acccatccag taaatggttc
     5101 aaataatcca tcactgcggc gttatcgttt ttcttgtgcg aggtggaaca tctcgcacgg
     5161 tttcttcatc aaccatcaac tcataaattc tgatgctgtt atcaccgttt gatacgtcat
     5221 caaatacctg aaaaagaaaa ctcatgaact gctcataagt gacgctgggt tcaacatacc
     5281 caccagtaac ctcaattaac ggtgttagcc ctgaattatc tgctaaagaa acgcagtcca
     5341 acttatccaa acggaaaaag taattaccgc aaggcgttct tttgtcctta atccgtataa
     5401 atacatttga taacgctgta tgactatctg agagcttaaa gatttttgcc gtgtccattt
     5461 tattcagccc tgtaagttgc ttatgatgga ttgcccagac ccaaccacat taaccaccct
     5521 tccctaattt ccttgggacg actctcatac gccattttca ttccgttgtt ccaggctggg
     5581 aggtagaccc agtactcacc tgcccgccct gaagttgatt gcggatcagt catctcaact
     5641 acgggtagct ttcctttttc aatcattccc cttactgctg ccggtgtttt gccgatcagt
     5701 tttgcaaact cctggtacgg gacagcatca ctaatactga caacttgttt gctcatctgc
     5761 taacctcgta tctagatcta accaatgggt ttcaaagttc tttaatgggc tttgatgcac
     5821 attgacaaca tctaaaacca cattagagag tttcgataat attagaggat cttgataaca
     5881 tgtcaacgcc cataagcgaa aaactagcgt tagttcgtga gtctgagcga ctaaaccgga
     5941 agcaattcgc tgaaataaca ggagttcctt acagttctct aacttattat gagagcggaa
     6001 gaactacgcc ccccactgac gtaacaatga aaattctcca gcaccccaga tttagcaaat
     6061 acacgttgtg gtttatgacc gatcaggttg ctcccgaagc cggtcagata gcaccggccc
     6121 tcgcacactt tgggcaagat gtaacaacat cgcagcactc agaccaaaag attggttaac
     6181 cattcaccag gcgcatcttt attacaaaat ttgtttactt gttgccaaat acaacaagca
     6241 caacaagttt gtgaaatcta agcgcaaggg aaaattaaca gcagcaaaga aaacacaacc
     6301 tagcaccatt catttggggg tcttatgaca attaagaaac tcgatgatgg tcgttatgaa
     6361 gtggacatca gacctgcagg gcgcaacgga aagcgcatcc gcaggaaatt tgacaagaaa
     6421 agtgaggctg tagcttttga gaaacacact caatacaacc atcacgataa agaatggttg
     6481 gctaaaccga cagataagcg gcatctgtct gaattaacga aagtctggtg ggatctgaaa
     6541 ggcaagcatg aagaacacgg aaaatctaat ttgggtaaga ttgaaatctt tacccggatc
     6601 actgatgacc cttgcgcgtt ccagataacc aaagcgctta ttagccagta cagcatgact
     6661 cgcagagggc aaggcataaa accggccagt attaaccgcg atcttacctg cctgagtggt
     6721 atgtttactg cgctaattga tgcggaattg ttctttggtg aacatccttt cagagggatg
     6781 aaaaagctca aggagcagaa accagaaacc ggctatctga cgcaggaaga aataacattg
     6841 ttgcttgcca atcttgacgg ggacaacaag aaaatcgcga ttctctgttt aagtaccggc
     6901 gctcgatggg gtgaggccgc gaaactgaaa gctgagaacg taatacagaa tcgggtgacg
     6961 ttcgttaaaa caaagacgaa caaaccgcgt actgtcccta tctctgagga agtggctgta
     7021 atgattgcgg gtaaaggtta tctgttccct gatgcctcat acccgaagtt caggcgaaca
     7081 atgaaagatg taaaaccaga tctaccggac gggcaagcaa ctcacgcatt gcgtcacagt
     7141 tttgctaccc actttatgat taacggcggg agtatcatca ccttgcagag aatactggga
     7201 cattcacgga tagagcagac aatggtttat gcgcactttg ccccggagta tcttcaggat
     7261 gctgtttcac tcaatccgct acgtggtggt gttgactccc agtgtgtcca cactgtgtcc
     7321 acagtagggt agtttattat ggctttcagt ggtcttgcgt gccgcacaaa cccgcattgc
     7381 accgctgaaa gcccatgtta cctaccctga aaaacgccct tacacgggct tattttttat
     7441 gcataagccc tatccctggt caccgtcttc cattgaccac atcgatagaa tctcccttca
     7501 tagcacgatg cctttcacgt aacggcatcg tgctcgcaca ggttccggct aagcacaacc
     7561 agaacgcgca tgtttgacgc ttaccaaaaa atattctcac tctccacatt tgaatgtcag
     7621 acgagcgacg ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct gcattttttt
     7681 gccctgagta acacagaaat gggctgcatc attttttact atattttctg caccagatat
     7741 tctgcccctg gctaaagaag cttcggcttc ggtgtagtat tggttatcga gtttacgctg
     7801 aatattactt ttatatgcaa gaccaaattt accgatactt gtctcatcat tatgcacagc
     7861 acaaccagac ataataaaaa tactaattaa tgatatagca gctatctttt tcatctcacc
     7921 ttcccccatt aaataccaac gacactctct agtgtttaaa tataataatg gcatgattat
     7981 tataattgaa taggattata ataaatgttc tgtacaacat ttcctacata agtaggaatt
     8041 acggacattg aggcccttca gggtaactcc atgggggctt taatatattt ggaatagtat
     8101 atttaagatg ccactgtttg gttgaatatt aggcatatgc tcttttttga aatttatcgg
     8161 tggcagccgt tagtattcgc tgtccccatt gcaagctcct ggtggtaacc actgaatcct
     8221 ccatacttga actgactttt tatcctccga ctttcatcct gttctgactc caccttttgt
     8281 tttctgctct acactatcta cagaccaatc ataaaggcac atacgatcat ggcagaattt
     8341 cccgccagct tactgattct taatggcaaa agtactgaca atctaccctt gcgcgaagca
     8401 attatgctgt tgcgtgagga aggaatgacg atccatgtgc gggtcacctg ggagaaaggc
     8461 gatgccgcac gatatgtaga ggaggcccgg aagttgggcg tcgcaacggt gattgccggt
     8521 ggtggcgatg gcaccattaa tgaagtttct acggcgttga ttcagtgtga gggggatgac
     8581 atacccgcgc tgggaatttt gccattagga accgccaatg attttgccac cagtgtaggg
     8641 attcctgagg cactggataa ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata
     8701 gatatggcgc aggtcaacaa acaaacctgt tttattaata tggcgacagg cggatttggg
     8761 acgcgtatta ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac
     8821 atcattcatg gcttaatgcg catggatact ctgcaaccgg accgttgtga aatccgcggt
     8881 gaaaactttc actggcaagg tgacgccctg gtcattggta ttggtaacgg gcgtcaggcc
     8941 ggtggcggtc agcaattgtg tccgaacgcg ttaattaacg atggcttgct gcaactgcgc
     9001 atttttaccg gcgatgaaat tcttccggct ctcgtatcaa ccttaaaatc tgacgaagat
     9061 aacccgaata ttatcgaagg cgcttcgtcg tggtttgata tacaagcccc acacgaaatc
     9121 acttttaatc ttgatggcga accgttgagc ggacaaaact ttcatattga aatacttccg
     9181 gcggcgttgc gttgtcgatt accaccagat tgtccattat tgcgttaatc aaactattct
     9241 ctcttgaacc agggagaatc cgaaatgccg gatgcggtgt aaatacctta tccggcatac
     9301 agaacaatac tacaccgtaa ttaattttac ccgtgtcttc tgcaatgcca gtttatccac
     9361 ctctgataca gcatcatcgg taataataat gtcaattttt tcgattggca gtacctgatt
     9421 aaaaccgcga cggttaaatt tcgatgaatc gagaacggca atcactttat tagcggcggt
     9481 gaccatcgca ccgctaatcg aataaccttc attaaaggtc gtaataccat taaccgcatc
     9541 aataccatca gcaccgacaa acattaaatc ggcattaata tcctgcaggg aacgttcagc
     9601 aatagatcca tgcatagatc gcgttttatg gcggaccgtg ccaccgcaga cgaccagagt
     9661 aatgtcttta ttttcggaaa gggcaaacgc agccgggaga ctgttggtaa tcaccgtgat
     9721 atttttggcg gtcattaatc cttcggcgat aagcattgtg gtacttccgc tgtcgagaat
     9781 aacggtcatc ccttcatgga tcattttgac tgccgcctgc gctattctgt ttttcggcgc
     9841 gctggcgagc tgaaatcgct ctttaaaccc gacttcctgg gtctcggttt cactattacc
     9901 ggacattatt ttcgccgcac cgccatgaaa gcgcgtaacc acgccttttt gttcgagaaa
     9961 gcgcaaatcg gcacggattg tcgcttccga ggcagcaaat actcccgcca gatcctgaac
    10021 aagcacggtt ccctgttcat tcactaattg gatgatctta ttccttcgct cgaatgaatt
    10081 catcgtcatt tacccatatt caattgtggc tagtgtaaac gaagggggac cattggtgaa
    10141 tgatctgttt tgcacaatac cgggccagca tcacgctggc ccgcggtttc agggaatgag
    10201 caacactttg cccggcatag cattacgagc gatgtcacgc accgcctggg cgaagctttc
    10261 aaagcttcca cggtgagcga ttaatggctc caggcttaac ttacgttctg tcagcaaccg
    10321 gctcgccgtt tcccactcct gccccggcca agggctggag tagttcatcc aactgccgat
    10381 aaccgtcagc tctttacgca atattttgcc aaacgttgcc gatgttaaat gcagatcctg
    10441 atgcaacgtg cccaccagcg ccagttgggc atgaggaccg gcaatctcta ccgccagttc
    10501 gacagtttgc ggtacgccag ccgtctcgag gataagctga ttaaagcgca gttcgcgtaa
    10561 aacgctctgc atttgcggcg cgctcatttc gctactgtta aatgtttgca tcgcaccgaa
    10621 agattttgcc agtgccagtt tttctgaact aatgtcgatc gccgtcacac tctttgctcc
    10681 cagcgcgaca gcgcactgaa tggccagcag gccaatggtt ccggcaccaa taataataac
    10741 gtttttattc tcacaacctt gcgctaaatg aaaagcatgc aggccaacgg taatcggttc
    10801 aataaaagcc ccatcctcaa taggcatatc agtaggtaga gcaaagacat ttttccgctt
    10861 aacgacaata tattcggcaa atccaccatc acgccgtgag ccaataaaat catattttgc
    10921 gcactgggaa taaaaccctt tcaaacactc tggacaagta aaacagggta ataacggcac
    10981 acaggcaacc gcatcgccag gatgtaaatc atcaacaccg gatcccaccg catcaatata
    11041 gccgctaaat tcatggccta acgttattgg ataataatgt gcaccatttt taaatatcct
    11101 gggtaaatcg gaaccacata agcccgagct ggcaattttt acccgcacct catcctgatg
    11161 tttaatttca ggaatgacgc tttctgcaac gagcacgata ccatcagtat cattcaccac
    11221 tgatttcata aaaactcctg attgttaagg gggataacct ccccctcagg taaaaattat
    11281 tctgcgagaa cgactttctc ttgtttaata aagccacgcg ctctacgcca ggtcatgaaa
    11341 ataccggtaa gataaattgc gccgataata atgaatccgg gaatattttg cggggagaaa
    11401 acctgaatca gtaaccaggt aatgggagaa ccgccctgat ccattgaagc caccataccc
    11461 ccggctttta acgccccagc attagccgcc agttgggtgt gcaaaccaat agtttgcgtc
    11521 gcgatccaca gagtgatgct catgatgatg acacccgaga ttaaggtgcg gaacagattt
    11581 ccacgatgca cggcgactgc catcgccaca aagaagccga tggtggcaag atcgccaaac
    11641 ggcagcacct gattacccgg aacacaaaca gcaattaaaa tggtgagcgg gataaaaatc
    11701 aggcttgccg ataccaccgc cgtatgcccc agcagtaatg cgggatccag gccaatcagg
    11761 aactcctgac cgccgaactt cgcctgtaaa cgactacggg cctgcttagc gatgggcgtt
    11821 aaaccatcca tgatgggttt taatcacccg tggcattagc agcatcactg ccgccgtttt
    11881 taccgccagc tgcaatacac ctttgacatc gtaacccgcg aggatgccga taatcagccc
    11941 catcacaaaa cccacggtga caggctcgcc aaatggacca aatttgcgct gaatatcgtc
    12001 ggcgctaaat ttaattcggt taacgcctgg gattttctcg atgatagcat cgaccagcac
    12061 cgcaatcggc cccatatacg ccgacgtacc gtgcggaata gcaatacctt ccagctcaaa
    12121 gaaatttcgg gtatcgcggg caaaccagtc gccgagctta taaacaaacg ccgcgtgaat
    12181 taccacacct gccatcccta tcatccatga accggttgcc aggtgcagca acgcgccggt
    12241 gaaggtcata tgccagatat tccagatatc aacatttacc acccgcgtca tacgggtcag
    12301 taacatcgcc acgttaacca atatcgcaat cggaatcgcc accagcgcaa tttgcgacgc
    12361 ccaggtcatt ggtgaagagc ccggccagcc gacatcgacc acatgcagat tcaggtcgaa
    12421 attttccgcc atcgctttcg ccgccggacc aatggaatcc agcattaagc caatcacaag
    12481 gccaatgcca acaaagccaa tcccgatatg cagacccgct ttaaagcaat cgcctgcctt
    12541 catgcctaat attttagaaa aaataatgat gacaaccggc agcatcactg tagggccgag
    12601 gtcgagaata taacgcatga cttctgaaaa cataggtcac ccctgtaaga tagtcagaat
    12661 tttattttgt aatgcttcga taccgacacc agaaacaaaa ggcatgccgt gaactaacgg
    12721 aatatcgcca aaactgcgat cccccctggc agtggtacat atcaaatgca caccatccat
    12781 ataggtttct atttcattaa cccgacactg gattaattca acaggaatat tatgactctg
    12841 acacaactct ttaatttctt ccgccgccat cgtagaggtc gcaaccgcgc ctccgcaagc
    12901 gacaataatc ttgcgtttca taaatagtcc tttttattgt tacagaggga tttattcgtc
    12961 tgaatctaaa acatactttg tgaagtattc ctttaactgg gtttcaggaa gagtgattag
    13021 tgtctcgacg atatcgggct gttgtaactt gccaaataaa cagcgtaaaa gtttcaattg
    13081 ctgctgcgga ttttccacaa tcaacgcaat aaccaacgat accgccacgt cgttatcatc
    13141 atccgcttgc tgaaaatgaa ctttatttgt tggccttaac agataaatgg ctgacgactt
    13201 agcatgaatc gcctcacaat gcggtattgc aatggcgtgc tgctcaagca ttatcccggt
    13261 agggaattct gcttctctgg caattaatgc ctgtggccag gtatcatgaa ccacaccttt
    13321 ggcgagcatc tcattaccga tatgggttaa aacttcgcta cgatcgacaa aagaaattcc
    13381 gctacgaaca aacaggttag tcatataccg tccttattcc gcacagccgt agcgataggc
    13441 gcgcaaaaca tcataaattt tatccataat cagctgatgc ggtattgctg ataattcccc
    13501 ggactgaatg cgttcaaatt gtttgggaag atactgacta atcatgccca gtgggatgtc
    13561 cacgccttca agattcacca tcatcgtttc gacgctattt ttaatccgac tatgcggcca
    13621 gtaataacga atacgatccg acaggctgta acgaatatcc agtaatgaat cgttaaaacc
    13681 cgtacgataa tattttttcc agtattgcgg ttcgtcgagc atcacttctt caattaccgc
    13741 caggcaaccg ctgcgatttt caggggcgat aagttcctgc tcaatttgtg ccagtgcaaa
    13801 tatcgcctcg cgtaaagcaa aggttaatgc gggaccgact ttcaatattg caaagtgatc
    13861 gcggactaat tcccaataag ccgtccgggt ctggtaatcg gtagaatgtg cttcataaac
    13921 cattcgggtg ttttctatcc attgcgccag cggctgcgct tcctgcggct gataatggat
    13981 aatattgctg tgatcaaatt ccacgcccgg ctgcaccacg atggcaatca cgcgtgttaa
    14041 cgcctctgtc agcccacggg caataaaggc cttttgatgc gtacgtaaag tattggcggt
    14101 atcttcaaca tgggtgatgt gtactgactg aatggcgctg gcctcaccgc ccggaaccgg
    14161 aacttcggtg ccaatgacat agctcagttg ctcacgctgg caatctgtcg ccacactttc
    14221 cgcagcaaag caaagcacag cagctcgttc cgcaaccgtt tctggtgcta acggtatggg
    14281 atcccccgcg caggacattg acgcatcaag atgaatttta ctgaagccgg cacgaacata
    14341 ttcctttacc agctcgacgg atttttccat cgccgcatcc gcattttctt gctgccagca
    14401 gtttggcccc agatgatcgc cgccgagaat aatgcgttcg cgtgcaaacc caactttatc
    14461 ggcaatcgta aaaacaaatt cgcgaaagtc tgccggtgtc attccggtat aaccgccaaa
    14521 ttgattgacc tggtttgacg ttgcttcaat cagcactttg cgcgtgctgt tgcgatcaaa
    14581 tgccagcgcc gcttcgataa ccaacggatg ggcagaacag actgaacata tgccgatatg
    14641 ttcaccagct ttatgccggg caattaacgt tttcatgttt tttccttatt aaatggcagg
    14701 tgcgttatgc cctgccctag cagccacaat cggcaatcac tttgctcacc acatcgcgca
    14761 ttgcggattt agccgactgc aaataatccc ggggatcggt cgcttcaggg tgctcggtca
    14821 ggtaattttt taacgcctgc gagaaggcat ttttcagctc cgttgcaacg ttgattttgc
    14881 atatccccag tttgatggtt tgctgaatat ccttagtcga taaccctgac gcgccatgca
    14941 gcaccagcgg taagttcacc cactggcgaa tgttctccag tcgagaaaaa tcaagcgccg
    15001 gtgcgctggc atacatccca tgagccgtgc cgatggcgac cgccagggaa tcaattccgg
    15061 ttgcctcggc aaattcacgc gcctgagcgg ggttggtata aaaagcatcg gcttcattga
    15121 cttgcacatc atcttcctgg ccgccaagtt gccccagctc cgcttcgacg ctgacatcaa
    15181 agcgatggca aaaatccacc acctctttga cccgtgaaat attttgcgca aaaggcaaat
    15241 gcgaggcgtc aatcatgact gagcgcacgc cagaacgaac cttctgagcg atatcgtcaa
    15301 atttcgtgtg atggtcgaga tgaattgcca gtggatggtg atattgcttc gccatcgcgc
    15361 tgaccagcgc caacagattt tctgtaccag catgagtaaa tgtgccaggc gttccggcga
    15421 tgatgaccgg cgcatgcagg ttggcagcgg tttccaccac cacttgcatc gtttcgagat
    15481 tgtgaatatt gaatgccgga accgcataac cgccgcgctg tgcgttgttc agcatctgct
    15541 ttgtcgatac cacgtacatt ttcatatcct gtcgtttgtt ttctatttca aaatataatg
    15601 aaatgatttg tttttaaatg tcgagataac gatcacaaaa acgacaatgt gaaaattgtt
    15661 cgaggagtaa aaggcaaaaa aaaacggcct cccgataggg aagccgtagc aaagtgcgcg
    15721 tgtttttatg ccggatgcgg tgtaaacata cgtggcagtg ctatgtaggc atgataagac
    15781 gcttccgcgt cgcatcaggc ataggttgcc ggatgcagtg taaacgcctt atccgtccta
    15841 cggggtggtg ctgttcgtat gaggcgtatc aggcaatagt aattttgcta tcgagataaa
    15901 cgtcctgcac ggcattaatc agtttcacgc cgtcagccat cgatttcttg aacgccttac
    15961 gtccaagaat cagccccatt ccgccagcgc gtttgttgat aaccgcagta cgcactgcat
    16021 cactgaggtc agtttcaccg cctgcagcac cgccggagtt aatcagccct gcgcgcccca
    16081 tatagcagtt agctaactga taacgcacca gatcaatcgg gttttcgctg gttaacttgc
    16141 tgtacacgcg atcgtcggta taaccgtaat taattgcttt atagccgccg ttattttccg
    16201 ccattttttg tttgacgata tctgcaccga tggttgccgc cagatggttt gcctgaccgg
    16261 tcaggtcggc ggaaacatgg taatcaacgc catctttctt aaaggcggag ttacgcaaat
    16321 aggcccacag cactgtcacc atacccagct cgtgcgcacg ttcaaaagcc gcagaaattt
    16381 cttcaatctg gcgacgtgac tcttccgagc caaaatagat agtcgcacca accgcaaccg
    16441 cgcccatgtt gaacgcctgc tccacgctgg catacagcgt ttgatcgtag gtattcgggt
    16501 aacttagcgt ctcgttgtga ttaagtttga cgaggaatgg aatgcgatgc gcataacgcc
    16561 gcgataccga cgccagcacg ccgtaagttg acgccacaca gttacagccc gcttcgatcg
    16621 ccagttcaac aatgtttttc gggtcaaagt agagcgggtt agcagcaaat gaagctccgg
    16681 cagagtgctc aacgccctgg tcaaccggca gaatagaaag atatcctgtg ccagccagac
    16741 gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc tggcgggcga ttattgtcaa
    16801 tcattacgcg gtctacgtag tcatgtccgg ggagataaag ctggtcagaa ggaattgtca
    16861 tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag caactgcgca atatctgtca
    16921 ttactatgct cccgtaaatt ccgattggat atcggctatg gattgtcctg gcccgccttt
    16981 tgcgggcaat cataatcctg gtcgttacgg gcgagttttt ccatcttttg gatgtttttt
    17041 cagcgttttc tgttggctcg attcatcaga aaaaatgtta gcgcggtcaa gttttcaccg
    17101 caaaggtatt taaaaggtat tattaagtgg tattatcatc gcgtacctta cattacctgt
    17161 catgaaggaa ttaaaagatg aaaacaacag caaagctgtc gttcatgatg tttgttgaat
    17221 ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg gttaagtaaa agcggtttta
    17281 gtgccggaga aattggctgg tcgtatgcct gtaccgccat tgcggcgatc ctgtcgccaa
    17341 ttctggttgg ctccatcact gaccgctttt tctcggcgca aaaagtgctg gcggtattga
    17401 tgttcgcagg cgcgctgctg atgtatttcg ctgcgcaaca gaccactttt gccgggttct
    17461 ttccgttact gctggcttac tctctaacct atatgccgac cattgcgctg actaacagca
    17521 tcgcttttgc caacgtgccg gatgtggagc gtgatttccc gcgcattcgt gtgatgggca
    17581 ctatcggctg gattgcctcc ggtctggcat gtggtttctt gccgcaaata ctggggtatg
    17641 ccgatatctc accgactaac atcccgctgc tgattaccgc cggaagttct gctctgctcg
    17701 gtgtgtttgc gtttttcctg cccgacacgc caccaaaaag caccggcaaa atggatatta
    17761 aagtcatgct cggcctggat gcgctgatcc tgctgcgcga taaaaacttc ctcgtctttt
    17821 tcttctgttc attcctgttt gcgatgccac tggcgttcta ttacatcttt gccaacggtt
    17881 atctgaccga agtcggcatg aaaaatgcca ccggctggat gacgctcggc cagttctctg
    17941 aaatcttctt tatgctggca ttgccgtttt tcactaaacg ctttggtatc aaaaaggtat
    18001 tattgcttgg tctggtcacc gctgcgatcc gctatggctt ctttatttac ggtagtgcgg
    18061 atgaatattt cacctacgcg ttactgttcc tcggtatttt gcttcacggc gtaagttacg
    18121 atttttacta cgttaccgct tacatctatg tcgataaaaa agcccccgtg catatgcgta
    18181 ccgccgcgca ggggctgatc acgctctgct gccagggctt cggcagtttg ctcggctatc
    18241 gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatcc ggaaccggta aacgggctga
    18301 ctttcaactg gtccgggatg tggactttcg gcgcggtgat gattgccatt atcgccgtgc
    18361 tgttcatgat ttttttccgc gaatccgaca acgaaattac ggctatcaag gtcgatgatc
    18421 gcgatattgc gttgacacaa ggggaagtta aatgaaaaca gaacgtattc tcggtgctct
    18481 ttatgggcag gcgttagggg atgcgatggg gatgccctcc gagctttggc cacgcagccg
    18541 cgttaaagca cactttggct ggattgaccg ttttctgcct ggaccaaagg agaataacgc
    18601 ggcctgttat tttaaccgcg ccgaattcac cgacgatacc tcgatggcgc tgtgtctggc
    18661 ggatgcgtta ctggaacgtg aaggcaagat cgatccggat ctgattgggc gtaatattct
    18721 cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta ctaggtccga cctcgaagat
    18781 tgcgcttaac gccattcgcg acggtaaacc cgttgctgaa ctggaaaaca acggcgtgac
    18841 caacggcgca gcgatgcgcg tctcgccatt aggttgtttg cttccggcgc gtgatgttga
    18901 ttcctttatt gatgatgtgg cgctggcgtc gagcccgacc cataaatccg atctggcagt
    18961 tgcaggtgcg gtagtcatcg catgggcgat ttctcgtgcc attgacggag aaagctggtc
    19021 agcgattgtt gattccctgc cttcaattgc gcgacatgca cagcaaaaac gcatcactac
    19081 cttcagcgcc tcactggcag cacgtctgga gattgcgctg aaaattgtgc gcaatgccga
    19141 cggcaccgaa tccgccagcg aacagcttta ccaggtcgtt ggcgcaggta ccagcactat
    19201 tgagtccgtt ccgtgcgcca ttgcgctggt tgaactggca caaaccgacc cgaatcgctg
    19261 cgccgtcctg tgcgctaacc ttggcggcga cacagacacc atcggcgcta tggcgacggc
    19321 gatttgcggc gcgttgcatg gcgttaacgc tatcgatcct gcattaaagg cggaactgga
    19381 tgcggtaaat cagcttgatt tcaaccgcta tgccacagcg ctggcgaaat atcgtcaaca
    19441 acgggaggcg gtatgagcgg cgctcgatta cacacgctgc tgcctgaatt aacctcgcgt
    19501 cagccggtga tggtcgtcgg cgcggcggtc attgatgtga tcgccgacgc ttatgccctc
    19561 ccctggcgtg ggtgcgatat cgaactgaag cagcagagcg ttaacgttgg cggctgcgca
    19621 ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag cgggtaatgc cttgccgctc
    19681 ggtcagggcg tgtgggcgga gattattcgc aaccggatgg cgaaagaggg gttaatcagc
    19741 ctgatcgata acgccgaagg tgataacggc tggtgtctgg cgctggttga gccggatggc
    19801 gaacgcactt ttatgtcatt cagcggtgtg gaaaatcagt ggaatcgcca gtggctggcg
    19861 cgattaaccg ttgcgcctgg cagcctgctc tatttttccg gttatcaact ggcctcgccc
    19921 tgcggcgaat tgttagtgga atggctggaa gagctgcaag acgtgacgcc gtttatcgat
    19981 tttggcccac gtattggcga tatcccggat gcattactgg cgcggatcat ggcctgtcga
    20041 cctttagtgt cgctcaatcg tcaagaggct gagattgccg ccgaacgttt tgctttatcc
    20101 gcagagataa caacacttgg cgagcaatgg caggagaaat ttgccgcgcc gttgatcgtt
    20161 cgtctcgata aagaaggcgc atggtatttc agcaacgacg cttctggctg cattccggca
    20221 tttccaacgc aagttgtaga caccattggg gcgggcgaca gtcatgcagg tggcgtactt
    20281 gccgggctgg cctctggtct gccactggcg gatgccgtat tactgggcaa tgcagtggcg
    20341 tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa cgcgcgagga actactcctc
    20401 gcacacaaaa acgtatagat cgctgcgaca gtggctaatg ctgtactcaa taggccgttg
    20461 ctgttggtca agcgccactt gcttgatcac cagcaccggt attttgctat ctaactgaat
    20521 atgtgactga aactcggcat ccggcatccg ggcgctaacg cgggaacgcg tacgttgtag
    20581 gtaaatgtgc tggctgcgga aatagtcata aagtgaaatc ccgatggcat caacatcatg
    20641 aattaaatgc gccggaaccc acgattcctc aatcgatacc gcttcttcat cgacatagcg
    20701 aatacgctta agcaaaaaga catcacttcc tgcttcgacc gccagctgct gcgcgacttc
    20761 ttcgggacat ttcacgacac gcttgttaac ccatagcgta tcgggctttt tcccgcgcaa
    20821 taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag tattcgaaga tgttgttgat
    20881 ctgcgtgccg taaccgcgcg aacgcgtcac cacaccctct tcttccagcg cctgcatcgc
    20941 cttgcgcacc gtaatgcgcg aaacgccggt taactgactt aaatcacgct caccgggcaa
    21001 aatattgcca tgctccagca ccccgctgcg cacggcattt tttaccgttt cggcaaactt
    21061 aatataaagc ggcgtgttat ccgccgctaa aatacgctcg ttcaactgag cgataagctg
    21121 ggtatgcgct tgttccattt gtctttttcc tgccggggag tctggtggga gtatatcact
    21181 gcttcttatt taaggcgttt ctttaatccc atcaacgaat ccctgcaact cctccaccgt
    21241 gccattaaac acattaaaat ccaccggacc attgatgcca tctacctgtc cacggtcgga
    21301 atactgccag aagcgccagg ccattccgtc attgtccgga cgacgttgat aatagtgagc
    21361 cacccaccac ggatattcat taaaatagcc cgccagattg gtgtgataaa aaacggttcc
    21421 tgagtaaata atcggctttt ttcccgtact tttttcgacc atttttagcc actgacttac
    21481 tcgtttgcgt aattcttttg ccgataattt tccccgttct tctacgtcca gtacagcagg
    21541 gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat cgtgcctgaa ccggcgctgc
    21601 taccgacggg gagaaataat gatacgcccc gcgcagcagg ccattttcgc ggcttagttg
    21661 ccagttacgc gaaaaatacg ggtccaccag cttttcgcct tccgtcgcct taataaaagc
    21721 aaactgtaag cggataccgt tgtcgcgcat ttttgccacc cgctgccagt cgatccgctc
    21781 ctgccagcga gaaacatcta ttccgtgaat ggtgtaactg gcaggaatgc gaatagcgaa
    21841 agatttaacc gggcgataac cgtagaaatg gatgtagtct gtaatctgaa ctgctgtcga
    21901 atagaaaaaa ttcaccgtct gacgcggata aattgcaacg atggaaatta gcccaagagc
    21961 gcataataga gaggtgagtt tttttcgact ggtaattctt agctgcattg gtttcatccc
    22021 tgaatgtcag tgccaaaggc tgacaataac caaagcagct atagtacggt gctcaccacc
    22081 aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac ttccagcgtg tcggcctggg
    22141 ctaatgctga tgaaagccag ctttttgcct cctgtacggt gtcagcccag tttgtatggc
    22201 gcgggcgtag tgcagccaac gccgcagaga gcgtacaacc agtgccgtgg gtgtttttgg
    22261 tcataatgcg cggtgcggta aaccgttgtt caccctcgcg ggtaaacagc cagtccgggc
    22321 tttgctcatc atccagatga ccacctttca ttagcactgc gccacagccc atcgccaaca
    22381 gcgatcgccc ttgttccagc atttcctgtt cggtgcgcgc gtgtggcgcg tcgagcaagg
    22441 cggcagcttc gggcaagttt ggcgttatta atgaaacctg tggcaataat cgactgcgca
    22501 gcgtagcaac cgccgaaggt gaaagcagcg ggtcgccgct ttttgccagc ataacggtgt
    22561 cgagtaccac gttttggatc tgataacgtt gcaaccgttc tgccaccgct tcaacaatat
    22621 cggtttccgc caacataccg attttagtgg tgtcgattcg cacatcgctg aacaccgaat
    22681 cgagctgggc ggcgacaaaa tcaggctcaa tgcgatacac cgactgtacg ccacgggtat
    22741 tttgcgccac cagtgcagta ataactgagc aaccataagc gccaagtgcc gagaaggttt
    22801 taagatcggc ctgaatcccc gcaccaccac tcggatcagt accggcaatc gtcagagcgt
    22861 taattcgttt catgcctgca cctcctgcgt caattgccag agcgcatcaa ggaaatgtgg
    22921 aacaaaactg cctggcccct cgcttctggc gactgcgcgt tctccggctt gtttcatcca
    22981 gtgacaggca gatgcgacat tttccagcgt atcgcctggt aacgcacagc aggcagcgac
    23041 aaccgccgat aatgcacagc cagttcctac cactttggtc attaacggat cgccaccgtg
    23101 aatgccaacg gcgcgatggc catcggtaac ataatccacc tcgccagtga ccacgacgat
    23161 tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc gcgttagctg cggcgtcagt
    23221 ggtatccact ccccgtccac cattagcaat gccagctaat gccatgattt ccgaagcatt
    23281 accacgtatc gctgccggtt taaaagataa aagttcatga caaaaatggc ggcgataatc
    23341 gagcgcacca accgctactg gatcaagcgt ccagggtgtt tgagagcttt ttgcttgctc
    23401 aacggcagca cgcatcgcct gagcgcgtgg ctgcgtcagt gtgccaacgt taatcaacaa
    23461 ggcactggcg atagccgcaa actgactggc ctcttcggtt tcgataacca tcgctggcga
    23521 tgcaccgagc gccagcaagg tattggcagt aaaggtttgc accacatcgt tggtcatgca
    23581 gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgcg tgcgcagatt gcgctgaact
    23641 cagcaggtcg acttgcatag tttgctcctg ccataacgtg aagaagcaat gacctggtgg
    23701 tccgtgactt ccctacgctg gcattatcca gatcaggtga tacgggtatt tctcagcctt
    23761 cacgcagaag ggcaccccga gtcgtttggt tgcgatgata aatcgcagag gaggatggta
    23821 atgtccagcg cacgcgttgt aaacgaggaa aaacggcagt tttacaatcg cgttaataaa
    23881 ttatttgata tatgaatcca gcaccttcag aacgacatcc agatcttctt cacgttttag
    23941 ctcatccccc tggtgaacga tgtgttccgt cagatgacct ttaatcactt cccgcatcag
    24001 accgtttacc gcgccacgga tagcagcaat ctgttgtaaa actgcagcgc attcgtgcgg
    24061 ctcgtcgagc attttcttga gcgccacgac ctggccctga atcttactgg cacgcgcttt
    24121 cagtttctgt ttatcacgga ttgtatgaga catggcaaca cctggttaac aagaatatga
    24181 aaaatcatag cactattaat ctactggggg gtagtatcag gtactggggg ggagtagaat
    24241 cagattgccg aattaatact aagaattatt atcatgaccg aatttacaac tcttcttcag
    24301 caaggaaacg cctggttctt catccccagc gccatcttac ttggtgcgct tcatggcctg
    24361 gaaccagggc actcaaaaac gatgatggcg gcgtttatca tcgccatcaa aggcaccatt
    24421 aaacaagcgg tgatgctcgg actggcagca actatttcgc ataccgcagt ggtctggtta
    24481 attgcctttg gcgggatggt gatcagcaag cgctttactg ctcaatcagc agaaccgtgg
    24541 ctccagctga tttccgcagt gatcattatt ggcaccgcgt tctggatgtt ctggcgtacc
    24601 tggcgcggcg aacgcaactg gctggagaat atgcacgagt atgatcatga acatcatcac
    24661 cacgatcacg aagatcacca cgaccatgga catcatcacc atcacgaaca tggcgagtat
    24721 caggatgccc atgcacgagc ccatgccaat gacattaaac gacgctttga tggtagagag
    24781 gtcaccaact ggcaaatttt gttatttggc ttaaccggtg gtcttatccc ctgtccggca
    24841 gcaattaccg tgctgttgat atgcattcag ttgaaagccc tgacactggg cgcaacactg
    24901 gtcgtcagtt tcagcattgg cctggcgtta acgcttatca ccgtaggcgt tggcgcagca
    24961 atcagcgttc agcaggtcgc aaaacgctgg agcggattta acactctcgc taaacgcgcc
    25021 ccctattttt ccagtctgtt gattggctta gtcggtgtgt atatgggcgt acatggcttc
    25081 atgggcataa tgcgataact caatccttta ggcttgcatg ttatcttcag cccaggatga
    25141 ttcttaaatc agctatttcc gctgactctc tgctcgctaa ggatgctttt agggcatcct
    25201 ttcatttaca ctttttacga aatcatggga tcactaacaa aatatcgctt gtcagttata
    25261 ttgtatggca ggaaagatat gcgactgata ttacagatcc ccaaagtgga gagtttatga
    25321 ccattaaaaa taagatgttg ctgggtgcgc ttttgctggt taccagtgcc gcctgggccg
    25381 caccagccac cgcgggttcg accaatacct cgggaatttc taagtatgag ttaagcagtt
    25441 tcattgctga ctttaagcat ttcaaaccag gcgataccgt cccagagatg taccgcaccg
    25501 atgagtacaa cattaagcag tggcagttac gtaacctgcc cgcgcctgat gccgggacgc
    25561 actggaccta tatgggtggc gcgtacgtgt tgatcagcga caccgacggt aaaatcatta
    25621 aagcctacga cggtgagatt ttttatcatc gctaaaaaaa gccccctcat catgaggggg
    25681 aaatgcagac accttgttat tttttattat tagccacttg ctcgtcttgc ttgttattag
    25741 tcgtatttca cgttgattaa tgcggttgcc tccagtgcgc cagatttaac tttgtttgta
    25801 tcgtagacgt agtaactggc agttatcgga attgaaaccg ttgtatctgt aagttttgac
    25861 tttgccgcaa tttcttttaa atttgtgtat cctttactgc tgttagtgat atttaaaagt
    25921 gtgactgacg aaccattact ttcaagaata aatcctacgc cattatcttc cgcacctttc
    25981 agtattgtgt tgctgttggt taacaagtca gaacttgata aatacacctg cattttccgt
    26041 gatgtttgat tttgttgacc taataaattt ttacaacgca gaataatatc cccttgttta
    26101 ctatttattg ttgctttgtt acctgtggct ggtaattgtg aaacgggaat actgtctaaa
    26161 gtaattaata aatcgtccgg tttgcaggtt gtggttatcg ggttgtatga caatgtcaga
    26221 ttaaaggaat agacacctgc attttgtgcc aatgcatttc tgtagctatt tacacatgca
    26281 tcccatgact tgccacctaa taaacacagt agaaattgta ctgcggcact cccgcgtgtc
    26341 gatgatgaag cactggataa tgtcgcaact ccattaatca tcactgaacc gccgcccccc
    26401 gcagaaacat tcacttttgc attactggcc gcaggtgtca ccgttaccga ataaccactg
    26461 gtgaaactgg tctgattatt tgataacgtg atgttgtttt tattggtcca ttcaaaatgt
    26521 aaatttaatt taatggtatc gttataaagt ccaacaataa tatccgacgg gctcataggg
    26581 gtgatagtat ttgtactcac gcatgacgaa tcattaaaat tatgctcata tgtcgccatt
    26641 tgtgtttgag taagtgcaaa atccccctga acaaccaaat tgtgctgaac agataactca
    26701 ctataacatg ccgcatagct cacaggcata accatcatca ttactataaa taacattata
    26761 cgaatattca taaccacctg actattactg gcaaatatag tttctacttt catcaatctc
    26821 tttaccgaag gtgattgtgc atgaaagtcc ttgttgctta tcaattgcta cattaaccga
    26881 tggcggtact tcattggtgc gaataaataa ctggcttccc tgaccgacaa cgccaatatt
    26941 atgaccatgg atatcattga cttcataacc aaacattaat ggttgcccat ccgctcttaa
    27001 cgcttttata aaccagggct tgcgctgatc ggtatcaaaa ttaaccagta caaccgcgcc
    27061 gcgataaggg gcggcaattt tccggttgcc acgtaattct gcttcgctat cgctttgcga
    27121 cacatccagc atcaggtgat tttcccgata aggtgtcatt ccgtcgtata ccaccactcc
    27181 attacggttt gttgtgcgat atttttgccc attgacataa gcatctttaa ttcctggcgc
    27241 attcatcaca gcaaacgttt cggaaagacg gttcgccaga ttaacgcccc ccgaccaggc
    27301 gacaatgccc cctgaaacac tggctccagc ctgtcgataa gtactcgact gactataact
    27361 gccattcact gtcgcaaccg gcgcgttcca ggtcaaattc gccccagctg tcgtttcatt
    27421 tccctgattc tgataactca ggttgacccc atagttaaac tgatcccggc ttccaacggt
    27481 tcctgataat cccgtattat ttgaggcaaa cccctgatca tcaaacgtcg ttgagttaga
    27541 catatatatt tgccgacgag gcgtcgtaac gtcatcaccc caatcaaagg gaatcgatat
    27601 aaaaatatta aaacgtttct cttcatgatg attctcgtca tacgcctggc ttgccgcgag
    27661 gatatagctt atccgccgcc agttattgga ataactcaac tgataatcct tactgctgcc
    27721 gctacgcccc cagtaatctc gccataacgt acttaaggac acagaccccc agccttctgg
    27781 caatgactgg ctcatattgg cagagaagct atttttacgg ccaaaatcgt tctggtaata
    27841 atcggcaatg tcatagacat cgttttcatc acggcgataa ttatctttat tgtttgccca
    27901 aacgtgatca ttaaatgtcc ggtaatcacg cgatgaataa cgccaggccg ccagaccaaa
    27961 acgcgtcgac gtttggctca caaatttgtt gtaggcaatt tgataacttt gcccgtcaaa
    28021 cacatcgccg ttgtcttgtt tactatgcga cttcgtggca tcgacggaaa tggcaccaaa
    28081 gcgtgtattc cagccagtcc ccaaagtaaa cgcgtaataa ttattcgcga ccatcgagcc
    28141 accatacagc gtcaataaat tattaaaacc atactgataa cccgcctgga caaaatcact
    28201 ttgtttgctc gccccttcaa tatggctacg acccgccgca aaatcatatt tcgatacgcc
    28261 gggttgcagc atatttggca ctgctgcata aggcaccaga taggtggtta ccgagccgtc
    28321 cgcctctttc acgctgacat caagatctgc tccaccaccg gccaactgca aatctgtaat
    28381 cgcgaacggg ccaggaggaa cctctttctg ataaaccaca aaaccgttct gttcaatagt
    28441 taccagcgca ttactctgag caatcccctg cacccgtggc gtaaaatttt gtttcgagtt
    28501 aggcaacatc tgcatatcac gaaacaaccg cacaccgctg aagcgaacag aatcaaaaat
    28561 atcgcttgat gtgtacatat cacccacgcg aagcgtgccg agaagttggg caaatccacg
    28621 ttccagatac agggtattgc ttttccacac ccctggattg ttatttgttt tactgaaact
    28681 ggcatcagaa tgcagttgcc accccagtaa atttaacccg ctgttaaaac gtacatatgt
    28741 actcttacta ttacccgacg ctttatagtc actgtaatac tgactcacat aataagaggt
    28801 ataaaacgca ttaatacccc gctcccagtt ttccggcgga acatagccac tttccagttc
    28861 ttccacccag gcctgcggga cactgaaatc gagacgaaaa accccgatat cccaggtata
    28921 gctcctaccc tgaacaagtt gctcaaatgt taaacattgc ttaccgctgg cgaagttatc
    28981 gctattaatg cctaaccgct tgataacttc tattgataaa catgtttctt gcgggttgtc
    29041 tttaacaata atctcatatt tcccgcgcca ttgcttattg acataaatat cgatgtcata
    29101 ctgccccggt aagggttgat tatcatcaag acgaatattt gcaacctgct ggtctttcat
    29161 tccacctatc ataaaatggg tatcaaaggt ttcttcagct gcataagctt caatgccgag
    29221 caataacgct acgattgctg atgcaagtgg ggtcattctc aacataggca gctcctgcaa
    29281 ttaaattttg tcactaatat agttgccgtg gtcatcgata atggtcagat accagttatt
    29341 tgcattatta cttttgacat taacactctg actttctaag ggggcaatca taatagtttc
    29401 ataattgact ttaacattat tagctttgac atccgaaatc gtcacccaat tagctgagtc
    29461 attttttatc accaatccat tgccactgcg atttaccagt aactttttaa aggtcgcttt
    29521 atttaccgga gcaatacccg ctggccggta aaacaactta attctgtttt gcatcgcaaa
    29581 cttcagtgca tttttgcctt cttgctctgg actatttggt ggaatatcca gaacattcag
    29641 ataaaaaatg ctttctttat tagtgggcag tttattcggc ataattttga tttttacttg
    29701 ctgcccggaa tttgccccta tttttgccac tggtggcgtt aacatgaaag gaacctgaat
    29761 tttttctggt ggtaacgacg tatcgccatc atcaatccac gcctgcagca gcgaagaacg
    29821 gtttccctgg ttcatcaact gcaccataac ttctttattt tctgccgggt aaataatgcg
    29881 cgtcccgtag ataacaattc cggcatgtgc aggaaggacc atagaaaaaa tgagtaaaga
    29941 taataatcct ttcatcttaa tgcctcttaa attaaaaagc cgccatgggg atagcggcca
    30001 tgtttattac ctggagaaac ttacaatttt atttattaat cctgcgccac acgcataacc
    30061 agagaagata acacatcacc tgattgcact gagttatctg tcagtttcag gtattttgct
    30121 ttaaacggaa tagcatattg accttccgct gttttataac tctcaatgtt aaaaggtgca
    30181 gaaacatcca ggacatcgct gttatcacct aaatagagat agtaacccac ggtatcgttg
    30241 ctactggcta tcgccttatc gttaccggtt attttatcgt tggtaatcaa cgagagtttt
    30301 acaccagcaa cttctttcgg gcagctaatt tttaacatgc tgctggttac accgactgca
    30361 tcatcatagg tagaatattt agctttacct tcaaagacag tggttggtac agtcggcata
    30421 ttaatactta ctgaatcacc acccgcttcg aatttacacg gagattctgc aatattcccc
    30481 ttaatagtta atgttccggt atcaacgtct gcagcaaata ctccagcgct cataaaaaaa
    30541 gaagaaaaga cagcggcagc aataattgaa cgtttcataa tatgttcctt aataaaattt
    30601 atttttggtt gttaagagtc tgtatctctt ggaaacaaat ttctcacatc aaaaaagttg
    30661 ccgcaatgta aacacattgt tttattgatt taaaaaaaat caaccattgc aatataaata
    30721 caaaaaatat ttaag
//
LOCUS       DAZFYC010000052        29986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFYC010000052 DAZFYC010000000
VERSION     DAZFYC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.052"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHKW5_002594"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHKW5_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO7511966.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHKW5_002595"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHKW5_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO7511967.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKW5_002596"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKW5_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO7511968.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKW5_002597"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKW5_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO7511969.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKW5_002598"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKW5_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO7511970.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKW5_002599"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKW5_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO7511971.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKW5_002600"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKW5_002600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO7511972.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKW5_002601"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKW5_002601"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO7511973.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12704..13837)
                     /gene="thiH"
                     /locus_tag="ACHKW5_002602"
     CDS             complement(12704..13837)
                     /gene="thiH"
                     /locus_tag="ACHKW5_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO7511974.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGHASQRP"
     gene            complement(13834..14604)
                     /gene="thiG"
                     /locus_tag="ACHKW5_002603"
     CDS             complement(13834..14604)
                     /gene="thiG"
                     /locus_tag="ACHKW5_002603"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO7511975.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14606..14806)
                     /gene="thiS"
                     /locus_tag="ACHKW5_002604"
     CDS             complement(14606..14806)
                     /gene="thiS"
                     /locus_tag="ACHKW5_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO7511976.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14790..15545)
                     /gene="thiF"
                     /locus_tag="ACHKW5_002605"
     CDS             complement(14790..15545)
                     /gene="thiF"
                     /locus_tag="ACHKW5_002605"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO7511977.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15538..16173)
                     /gene="thiE"
                     /locus_tag="ACHKW5_002606"
     CDS             complement(15538..16173)
                     /gene="thiE"
                     /locus_tag="ACHKW5_002606"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO7511978.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16173..18068)
                     /gene="thiC"
                     /locus_tag="ACHKW5_002607"
     CDS             complement(16173..18068)
                     /gene="thiC"
                     /locus_tag="ACHKW5_002607"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO7511979.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18149..18249)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18301..18777)
                     /gene="rsd"
                     /locus_tag="ACHKW5_002608"
     CDS             complement(18301..18777)
                     /gene="rsd"
                     /locus_tag="ACHKW5_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO7511980.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18872..19645
                     /gene="nudC"
                     /locus_tag="ACHKW5_002609"
     CDS             18872..19645
                     /gene="nudC"
                     /locus_tag="ACHKW5_002609"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO7511981.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19685..20749
                     /gene="hemE"
                     /locus_tag="ACHKW5_002610"
     CDS             19685..20749
                     /gene="hemE"
                     /locus_tag="ACHKW5_002610"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO7511982.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20759..21430
                     /gene="nfi"
                     /locus_tag="ACHKW5_002611"
     CDS             20759..21430
                     /gene="nfi"
                     /locus_tag="ACHKW5_002611"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO7511983.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADTVASERPAFVRY
                     TANQP"
     gene            21473..22063
                     /gene="yjaG"
                     /locus_tag="ACHKW5_002612"
     CDS             21473..22063
                     /gene="yjaG"
                     /locus_tag="ACHKW5_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO7511984.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22250..22522
                     /gene="hupA"
                     /locus_tag="ACHKW5_002613"
     CDS             22250..22522
                     /gene="hupA"
                     /locus_tag="ACHKW5_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO7511985.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22535..23230
                     /gene="yjaH"
                     /locus_tag="ACHKW5_002614"
     CDS             22535..23230
                     /gene="yjaH"
                     /locus_tag="ACHKW5_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO7511986.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23232..23657)
                     /gene="zraP"
                     /locus_tag="ACHKW5_002615"
     CDS             complement(23232..23657)
                     /gene="zraP"
                     /locus_tag="ACHKW5_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO7511987.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGGHMGMGHW"
     gene            23895..25271
                     /gene="zraS"
                     /locus_tag="ACHKW5_002616"
     CDS             23895..25271
                     /gene="zraS"
                     /locus_tag="ACHKW5_002616"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO7511988.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25268..26593
                     /gene="zraR"
                     /locus_tag="ACHKW5_002617"
     CDS             25268..26593
                     /gene="zraR"
                     /locus_tag="ACHKW5_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO7511989.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTVYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26590..27879)
                     /gene="purD"
                     /locus_tag="ACHKW5_002618"
     CDS             complement(26590..27879)
                     /gene="purD"
                     /locus_tag="ACHKW5_002618"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO7511990.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27891..29480)
                     /gene="purH"
                     /locus_tag="ACHKW5_002619"
     CDS             complement(27891..29480)
                     /gene="purH"
                     /locus_tag="ACHKW5_002619"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO7511991.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7035 a         7810 c         8248 g         6893 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctaggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc ctggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccacgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc
    11701 aatccttgct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgccgaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaattttt aagaaaggca gggcgaaacg aggaagaagc ttttcgccct ggacaacatt
    12601 cctgctgacg gaactaccat aaaaaaacag aacgctttca acaaccttgc gtttttcatc
    12661 ttccgtagcg gctcggaata aatttacgtt gttacattct gtctcatggc ctttgcgagg
    12721 cgtgtcccag atagctgtcc cagtctttcc ataccggctg caaaccctga gcggttaacg
    12781 cggccgcaac tgcttctggt ctgcgatcgt cgtgcggtga gaactgttcc agctcggggt
    12841 gattgtcggc atagccacct ggctgcgttt tcgaaaaagc gctgacgtta ttaattgcca
    12901 gcggaatcac gcgatcgcga aaccacggtg attcccgcgt ggagagtgag agttcaatct
    12961 ccggtgcaag cagtcggaag gcgcagatgg tttgcactaa ctggcgttca tccataatcg
    13021 acgcaggctc aatgccgcca gtacacgggc gcaggcgcgg aaaggagaca gagtaacggc
    13081 tttgccagta atgctgttgc agccatagca aatgttcggc aaccatatag cagtcaacgc
    13141 gccagttgtc ggaaaggcca attagcgcgc cgaggcctat cttatcaatc cccgcacgcc
    13201 ccagccgatc cggcgtttcc agccgccaga agaagtcctg ttttttgcct ttcaaatgat
    13261 ggcgggcata agtcgcctcg tgatatgtct cctgataaac catcacgcca tccagaccaa
    13321 gctgctttaa ctcggcgtat tccgtctccg ccagcggttg cacttccatt tgtagtgaag
    13381 agaactgttc acgcagggcg gggagatgac gacgaaagta atccatcccc actttcgcct
    13441 gatgttcacc agtgactaat agcagatgtt caaagcccat ctcccgtata gcggcgcttt
    13501 ccctggcaat atccgcttca tccagcgttt tgcgtttgat gcgattactc atggaaaatc
    13561 cgcagtacgt gcagtcgtta gcgcaaagat tggaaagata aagcgggacg tagaaactaa
    13621 ctgtgttgcc aaatcgctga cgggtcagac gctgcgcccg ttgggccagt tgttccagat
    13681 agccactggc ggctggcgat aacagcgcca tcatatcgtc gcgggtgagt tgcgaggcat
    13741 ttagcgcccg ttctacgtca gcagccgttt tgccgttgat acgcaggcgg atgtcgtccc
    13801 agtccagttg tcgccagcga tcgctgaagg ttttcatgcc gatgcctcca gaaatccagt
    13861 cagcgggctg gtggcatgag caaaatgact gcggctgccc ggtccggact gacgtgccag
    13921 caggcctgct tctaccgcca gacgaaatgc cttcgccatg ttgacgggat cgtccgcgac
    13981 ggcaattgcc gtattcacta acaccgcgtc ggcccccatt tccagcgcct gcgcggcatg
    14041 gctgggaacg ccgatgccag catcgacaac caccggcact gtggcctgct ggataataat
    14101 ctccagcatg gcgcgggttt ccagtccctg attcgagcca atcggcgcgc cgagcggcat
    14161 caccgctgca cagccgactt cttccagacg tttacacaat accggatcgg ccccgcagta
    14221 aggcagcacg acaaatccct gttgtaccag cgtttcggcg gctttcaggg tttcgatggg
    14281 atcgggcaac agccagcggg cgtcagggtg aatctctaat tttaaccagt ttgtgcctaa
    14341 cgcttcacga gccagatggg cggcgaaaat ggcttcttcc gctgttttcg ccccggatgt
    14401 atttggcagc agggtcacac ccgccgcgat aagcggttcg aggatagcgt cgttgtgctg
    14461 gcgcaagtcg acacgtttca tcgccagtgt caccagctgg ctgccggaag cgcggatcgc
    14521 ctccaccatc agttgtgaag aagcgaattt ccctgtgccg gtaaacagat gtgaatcaaa
    14581 cgttttgtcc gcaatacgta acatttcaac cccctgcaat aacctgaaaa agcaggatct
    14641 ggtcgccatc ctgcacgata tgttgcgccc actgctcacg cgggacgatt tgctgattaa
    14701 tcgccagagc cgcgcccgct tgtcgttggt cgagttgctc cagtagttcg tgaacagttt
    14761 gcccggcggc gcactgcatc ggttgatcgt taaacaggat ctgcattgct tcctccgcat
    14821 accgggcaac cactggcgcg gcgcaacgcc aggctgcgcc actggctcga tttaccgtcg
    14881 aacagtcgga gttctcccgc aggtgtctct ataccgctta ataacttaat ggcttccagt
    14941 gcctgcaaag tgcccataac cccgaccacc gggccaacca cgcccgccgt gcggcagttg
    15001 cgttctggct cctggttatc tggccacagg cagcggtaac acccctgctc ccagggcggc
    15061 gtcagtacca tcaactgacc gccaaatccg accgcgctgg cggtgataag cggcgtgttg
    15121 agtgccacgc aggcggcatt aatctcctgg cgagtcgcca tattgtcggt acagtcgagc
    15181 accacatcgg cccgtgcaac cgcatctttt aacgcctcac ccgttaaccg ttgttgtaat
    15241 gctgtcagtt gaatgtcggg attcaactgt gtcagtcgct gttggctgac ctgcgatttc
    15301 gggctgttga tatcttcagt ggtaaagagg atttgtcgtt gcagattgct taaatgcaca
    15361 tcgtcgtcat ctgccagtac cagcgtcccg acgccagcgc ccgccaggta cagcgcagca
    15421 ggtgtaccca gcccgcccag accgataatc agcacctggc tgtcgagcag tttttgctgc
    15481 ccgtccagag cgatatcgtc gagcaggatt tggcggctat aacgcataaa gtcacggtca
    15541 ttcatcgcca actcctgcaa tttccagcaa ctgtgccgtt gccaaacgcc agtctgcagc
    15601 ttgagtaatg gcgctgacga cggcgatact gccgacaccc gttgctatca ccgcaggcgc
    15661 gcgtgccaga ctgataccgc caatcgccac ggtgggataa tccgccagtc gctcaacatg
    15721 ccgtgccagc tgttccagcc cctgcggtgc agaaggcatc tgtttggttt gcgtcgggaa
    15781 cacatgtccc agcgcgatat aagaggggcg tgctgccagc gcgacgtcga tttccatatc
    15841 gtcatgtgtc gaaacgccca gccgcaggcc tgccgcgcgg atggcattga gatcggtggc
    15901 ttgcaaatct tcctgcccca aatggacgcc atacgcctga tgcttgatcg ccagccgcca
    15961 gtaatcgttg ataaacaatc gcgcgttata gcggcggccc agcgcaattg ccgccacgac
    16021 atcggcttcc acctcttcat cgcgccgatc tttgatgcgt agctggagag tacgtacgcc
    16081 tgcatccaac agacgttcga tccactgtac gctgtccacc accgggtaca gtcctaaacg
    16141 aaaaggtaca ggaggaaaat caggctgata catcacgctt cctccttacg caggtagatt
    16201 tctccgcctc tggcacggaa gttctccgac atatccgcca ttcccatttc aatagtttgc
    16261 gtggcggcgt aatcacgcac ttcctggctg attttcatcg aacagaattt cggcccacac
    16321 atggagcaaa aatgggcgac tttacctgac tcttgcggca gggtttcatc gtgataagcg
    16381 cgggcggtaa acgggtcgag ggccagatta aactggtctt cccagcgaaa ttcgaagcgg
    16441 gctttcgaca tggcgttatc gcgaatttgc gcgcccggat gccctttcgc caggtcagcg
    16501 gcgtgggcgg caatcttata ggtgataagc ccctgcttaa catcttcttt attaggcaga
    16561 cccagatgct cttttggcgt tacgtaacag agcatcgcgc agccaaacca gccaatcatc
    16621 gccgcaccaa tccccgacgt gaagtggtca tagcccggcg caatatcggt agttagcggc
    16681 cccagagtgt aaaacggcgc ttcgtggcag tgctctaact cctcggtcat attgcggcgg
    16741 atcatctgca tcggcacgtg acctgggcct tcaatcatca cctgcacgtc atattcccag
    16801 gcaattttgg tcagttcgcc cagcgtatgc agctcggcaa actgcgcttc atcgttggcg
    16861 tcctgaatag aaccggggcg cagaccgtcg cccagcgaca gcgaaacgtc ataagcggca
    16921 cagatttcac aaatttcgcg gaagtgttga tagaggaaat tttcctgatg atgggagagg
    16981 caccatttcg ccataatcga accgccgcga gagacgatac cggtcaggcg tttcgcggtc
    17041 atcggcacat agcgcagcag tacgcccgca tggatagtga agtaatccac accttgctcg
    17101 gcctgttcca gcagcgtgtc gcggaacgct tcccaggtaa gatcttcggc gatcccgtta
    17161 accttctcca gcgcctggta gatcggcact gtaccgatcg gcaccgggct gttacgcaaa
    17221 atccactcgc gggtttcgtg aatatagcga ccggtggaga gatccatcac cgtatccgct
    17281 ccccagcgcg tggaccatac cagcttttcc acttcttctt cgatggaaga ggtgaccgcc
    17341 gagttgccga tattggcgtt aacttttacc aggaaattgc gaccaataat catcggctcc
    17401 gattccggat gattaatgtt ggccgggata atcgcacgtc cggcagcaac ttcatcacgg
    17461 acaaattccg cagtgatatt ttccggcaga tgtgcgccaa agctcattcc cggatgctgg
    17521 tggcgtaaaa cctcgctacg gatgcgctcg cggcccatat tctcgcggat ggcgatgaat
    17581 tccatttccg gcgtgatgat gccctggcgg gcgtagtgca gttgggtgac acggcgtcct
    17641 gctttggcgc gttttggtgt tagtacaccg ctaaaacgca gttcgtcgag gccatcatct
    17701 gccagccgcg ctttagtgta atcggaactg cgcacggtaa gttcttcggt atcgccgcgc
    17761 gcatcgatcc acggctggcg tagttttgcc agcccttgct gcacgttaat ggcaatctgc
    17821 ggatcaccat acgggccgga ggtgtcgtag accggaatcg cttcgttttc ttcgtactgc
    17881 ggctgttctt tgctaccgcc aattagcgtc gggctaagct ggatctcacg catcggcacg
    17941 cgcacgccgg ggtgtgtgcc agtgatataa atgcgttttg agttgggaaa ggcggtgcct
    18001 tccagggtgt cgataaaatg ttgggcccgg gcgcgttgtt cgcggcgggt cagttttgtt
    18061 gcagacatag ctcattccaa aaagttaagg acgtggcttg tcagacgacg gatgaagcaa
    18121 gagacgatcg ccgcaggggc gatgcgatag cagattaact cttgttccct tcgcaggtat
    18181 tagcctgatc aggttccgcg gatcccgaat aaacggtctc agccagttaa ggcactccga
    18241 caagaaatta gccccgcaaa tggggcattg aatgtaaatt acgcgttaac agcgcagaac
    18301 tcaagcagga tgtttgacgc gggcggcgtc aagcaccagc agaatcagct tatcttccag
    18361 cacaaagcgc gcttccagcg cctcgccgat gtcagataaa acctgttgaa actcaaggta
    18421 attatcatga tcgatggcgg tttccagact ggaatcgtag taatccataa tctgttgagt
    18481 attggcttcg agttgcggcc aaatcttcgc ggcgcgcgcg agttgcccgt tgccttccag
    18541 cttatgaaga atgcgctcat aaatactgaa atgtccggca gacaagtaat cgaccaggct
    18601 ctgacaaaaa tcatcaaggg ctttttcgtt tagcctcatg tacgattctt tgccaggctt
    18661 aatgccaacc agattgtagt aagccacgag cagatgctta cgtacatgta gccagcgatc
    18721 aaccagtttg ttacttcctc tgacgcgctc cgtcaggtta tcgagctggt taagcatgat
    18781 tgactccgca agtttgtatt caaaaactgc tcagtgagaa atgtaaaaac catgttaaac
    18841 atgccagtga tgcaaaggta gtgcaagagc tatggatcgt ataattgaaa aattagatca
    18901 cggctggtgg gtcgtcagcc atgaacaaaa attatggttg ccgaagggag aattgccata
    18961 tggcgaagcg gcaaatttcg atcttgtggg tcagcgcgca ctacaaatcg gcgaatggca
    19021 gggggaacct gtttggttag tacaacagca gcggcgtcac gatatggggt cggtacgtca
    19081 ggtcattgat ctcgatgttg ggctgtttca actggccgga cgaggcgtac aactggcgga
    19141 gttttaccga tcgcataaat actgtggtta ctgcgggcat gaaatgtatc cgagcaaaac
    19201 cgaatgggcg atgctgtgca gccattgccg tgagcgttac tacccgcaaa tcgccccctg
    19261 cattattgtt gccatccgtc gcgatgattc gatcctcctc gcccagcata cccgccatcg
    19321 taacggtgtc catacagtac ttgccggatt cgtcgaagtg ggcgaaaccc tcgagcaggc
    19381 agtcgcgcgg gaagtgatgg aagagagcgg aattaaagtt aaaaacttgc gttacgtgac
    19441 ttctcagccg tggccgtttc ctcagtcttt aatgaccgcg tttatggcgg aatatgacag
    19501 cggcgacatc gtgatcgacc cgaaagaatt gctcgaggcg aactggtatc gctatgacga
    19561 tttgccgtta ctcccgccgc ccggcaccgt agcgcgccgt ctgatagaag atacggtggc
    19621 gatgtgtcgg gcagagtatg agtgatgata cactgaccgc ctgacgcact aaggaacagc
    19681 caaaatgacc gaacttaaaa acgatcgtta tctgcgggcg ctgctgcgcc agcccgttga
    19741 tgtcactcca gtatggatga tgcgccaggc gggtcgctat ctaccggaat ataaagccac
    19801 gcgcgcccag gcgggcgatt ttatgtcgct gtgcaaaaac gccgagctgg cgtgcgaagt
    19861 gactttgcaa ccgctgcgtc gctacccgct ggatgcggcg atcctctttt ccgatatcct
    19921 caccgtgccg gacgcgatgg ggttagggct ctattttgaa gccggagaag gtccgcgttt
    19981 tacctcgcca gtcacctgca aagccgacgt cgataaactg ccaattccgg acccggaaga
    20041 tgagctgggt tacgtgatga acgcggtgcg taccattcgt cgcgaactga aaggcgaagt
    20101 gccgctgatt ggtttttccg gcagcccgtg gacgctggca acctacatgg tggaaggcgg
    20161 cagcagcaaa gccttcaccg tgatcaaaaa aatgatgtat gccgatccgc aggcgctgca
    20221 cgctctactc gataaactgg cgaaaagcgt cactttgtat ctgaatgcgc agattaaagc
    20281 cggtgctcag gctgtgatga ttttcgacac ctggggcggc gtgcttaccg ggcgcgatta
    20341 tcaacagttc tcgctttatt acatgcataa aattgttgat ggtttactgc gtgaaaacga
    20401 cggtcgccgc gtaccggtca cgctgtttac caaaggcggc ggacagtggc tggaagccat
    20461 ggcagaaacc ggttgcgatg cgctgggcct cgactggaca acggacatcg ccgatgcgcg
    20521 ccgccgtgtg ggcaataaag tcgcgttgca gggtaatatg gatccgtcga tgctgtacgc
    20581 gccgcctgcc cgcattgaag aagaagtagc gactatactt gcaggtttcg gtcacggcga
    20641 aggtcatgtc tttaaccttg gtcacggcat tcatcaggat gtgccgccag aacatgctgg
    20701 cgtgttcgtg gaggcagtgc atcgactgtc tgaacagtat caccgctaag gagtgattat
    20761 ggatctcgcg tcattacgcg ctcaacaaat cgaactggct tcttctgtga tccgcgagga
    20821 tcgactcgat aaagatccac cggatctgat cgccggagcc gatgtcgggt ttgagcaggg
    20881 cggagaagtg acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg agctggtgga
    20941 gtataaagtt gcccgcatcg ccaccaccat gccttacatt ccaggttttc tttccttccg
    21001 cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg atttagtgtt
    21061 tgtcgatggt catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggctt
    21121 attggtggat gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta aattcgaacc
    21181 gctctccagc gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg
    21241 ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc atcgggtcag
    21301 cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc
    21361 aacgcgctgg gcggacacgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa
    21421 tcagccctaa ttcaggtaaa ctgcgggtaa tttccgtatt tgagaactca acatgttaca
    21481 aaacccaatt catttgcgtc tggagcgcct ggaaagctgg cagcacgtca ctttcatggc
    21541 ttgcttatgc gaacgcatgt accccaatta cgccatgttt tgccagcaaa ccggttttgg
    21601 tgatgggcaa atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga
    21661 cgcaaaagta aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga
    21721 cgatttcgat ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact
    21781 ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc
    21841 catcacgacc gttgcgatgc tggaaatgac tcaggctggt cgtgaaatga gcgatgaaga
    21901 gcttaaagaa aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact
    21961 tttagctgag tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg
    22021 tgaggcgggt gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt
    22081 taacgcctga tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg
    22141 gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc
    22201 cgtcgcactc gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac
    22261 tcaactgatt gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc
    22321 tctggagtcc actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact
    22381 ggttggtttc ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca
    22441 gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc
    22501 actgaaagac gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt
    22561 ttcacccctt ttgtctttct ggcgtcgatc attgatgctg gcaggcgctc tgtttctcac
    22621 tgcctgtagt cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca
    22681 gggcgcggta cgcatctggc gaaaagacag cggcgataat gtgcatctgc ttgccgtgtt
    22741 tagcccgtgg cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct
    22801 cacgctcatc aatatcaatg tttacagcaa accgccggtg aacattcgcg cgcgttttga
    22861 cgatcgcggc gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc
    22921 taacgaccaa atcgatttat accgttatcg tgctgatcag atccgccaga ttagcgatgc
    22981 cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt
    23041 gaccacctgc gaagggcaaa ccattaaacc tgatttagat tcgcaggcga tagcgcatat
    23101 cgagcgccgc cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga
    23161 aggttcgcaa ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa
    23221 aacgttctga tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtaa
    23281 cccatgccca ttccggtacc gcgcggaata cccgcttcag ccatcgcaat atctcgtttc
    23341 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    23401 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc
    23461 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    23521 gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga
    23581 gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    23641 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    23701 ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    23761 gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    23821 cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc
    23881 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    23941 gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc
    24001 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    24061 ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc
    24121 agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    24181 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    24241 attccccgca ggaaatgcag cagttacatc ctggagatga agaagcgtgg cggcggatcg
    24301 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg
    24361 ctggaatgca ccggatgcgc catatgcagc agtatgccgc aacaccacaa gcaattttca
    24421 tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    24481 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc
    24541 gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg
    24601 tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga
    24661 ttaaaggact ggcgaaatac tttgccgaac gcgcgccagc agggggagaa gcgcatcaat
    24721 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagttgctgg
    24781 aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc
    24841 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    24901 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    24961 atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggtca
    25021 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag
    25081 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg
    25141 ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc
    25201 aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc
    25261 cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg
    25321 cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    25381 gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg
    25441 aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat
    25501 tccggtgctg attatgactg tgtactccag cgtcgagacg gcggtagagg cactgaaaac
    25561 tggggcgctg gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga
    25621 aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca
    25681 gttcggtatg gtcggtaaaa gctcggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    25741 cgcgccatcg gaagccacgg tactgatcca cggcgattcc ggcaccggta aagagctggt
    25801 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    25861 tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt
    25921 tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt
    25981 tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca
    26041 ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat
    26101 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct
    26161 ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga
    26221 tatccctctg ctggctggcc attttctgca gcgctttgcc gagcgtaatc gcaaggcggt
    26281 aaaaggtttt acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat
    26341 tcgtgagctg gaaaacgcgg tggaacgggc agtggtgctg ctgaccgggg aatatatttc
    26401 cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc aaagtcagga
    26461 tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcactgg agaaaacggg
    26521 cggcaacaaa actgaagccg cccgtcagtt agggatcacg cgtaaaacgt tattggcaaa
    26581 actgtcgcgt tagttctgct cgcgttcgat agcacgccag ccgatatctt tccggcagaa
    26641 gcagtcgtcc cagtggatat cggtcattaa ggcataggcg cgtttctgcg cttctgccac
    26701 ggtatgaccc agcgcggtga cgcacagtac gcgtccgccg ttggttacca cctgctcgtc
    26761 atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt ccagcggcag
    26821 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    26881 gccgagagaa gcgcgttcat cccactcgga cgttttctcg tccagcttgc cttcacaggc
    26941 cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    27001 atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag
    27061 gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    27121 ccagataata cgttccatgg tgcgctgatg aacgtcatcg gttactaccg gcgcggggga
    27181 gtaagcgccc atcccgccgg tgtttggtcc ggtatcttta tcgcctacgc gtttgtgatc
    27241 ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc
    27301 ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc
    27361 cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc
    27421 tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata
    27481 cgccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg
    27541 ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg
    27601 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc
    27661 ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg
    27721 gatatcggtc acgccaatag caacgttttg cagcgcgggt tccagcgcag tgcctgcatt
    27781 acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc
    27841 gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg
    27901 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact
    27961 tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg
    28021 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact
    28081 tccaggcctt catcagcagc tttaataccg gcgattttcg cggagtacac gcggctcatc
    28141 tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc attggatttc
    28201 acgaacttcg ccactttcca gcagaacagc gcatcgcgca gttcctgttc ggtcggctga
    28261 cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc
    28321 agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg
    28381 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    28441 gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca
    28501 tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    28561 gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc
    28621 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    28681 tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag
    28741 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa
    28801 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accagcggct
    28861 tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg
    28921 tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc
    28981 agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc
    29041 acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata
    29101 tcgatgttct caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac
    29161 ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    29221 tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    29281 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct
    29341 ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa
    29401 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    29461 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    29521 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    29581 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    29641 attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt gtggataact
    29701 ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt
    29761 ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg
    29821 cggatgtgaa tcacttcaca caaacagccg gttcggttga agagaaaaat cctgaaattc
    29881 agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gcgctaaagc
    29941 gcgtcgcaac tgctctttaa caatttatca gacaatctgt gtgggc
//
LOCUS       DAZFYC010000053        29695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFYC010000053 DAZFYC010000000
VERSION     DAZFYC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.053"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHKW5_002620"
     CDS             complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHKW5_002620"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO7511992.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(805..1353)
                     /locus_tag="ACHKW5_002621"
     CDS             complement(805..1353)
                     /locus_tag="ACHKW5_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO7511993.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHKW5_002622"
     CDS             complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHKW5_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7511994.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHKW5_002623"
     CDS             complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHKW5_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO7511995.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRAPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHKW5_002624"
     CDS             complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHKW5_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO7511996.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGVFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDENEENQPDSGEEE"
     gene            complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHKW5_002625"
     CDS             complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHKW5_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO7511997.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHKW5_002626"
     CDS             complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHKW5_002626"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO7511998.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11027..11806
                     /gene="elyC"
                     /locus_tag="ACHKW5_002627"
     CDS             11027..11806
                     /gene="elyC"
                     /locus_tag="ACHKW5_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO7511999.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11783..12676)
                     /locus_tag="ACHKW5_002628"
     CDS             complement(11783..12676)
                     /locus_tag="ACHKW5_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO7512000.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHKW5_002629"
     CDS             complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHKW5_002629"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO7512001.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAAPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHKW5_002630"
     CDS             complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHKW5_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO7512002.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHKW5_002631"
                     /pseudo
     CDS             complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHKW5_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
     gene            complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHKW5_002632"
     CDS             complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHKW5_002632"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO7512003.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYSGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHKW5_002633"
     CDS             complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHKW5_002633"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO7512004.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFDHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17844..20108)
                     /locus_tag="ACHKW5_002634"
     CDS             complement(17844..20108)
                     /locus_tag="ACHKW5_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO7512005.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTIFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITTTDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAIHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGVRLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGRICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGMNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHKW5_002635"
     CDS             complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHKW5_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO7512006.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHKW5_002636"
     CDS             complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHKW5_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO7512007.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHKW5_002637"
     CDS             complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHKW5_002637"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO7512008.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHKW5_002638"
     CDS             complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHKW5_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO7512009.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHKW5_002639"
     CDS             complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHKW5_002639"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO7512010.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHKW5_002640"
     CDS             complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHKW5_002640"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO7512011.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26973..27665)
                     /locus_tag="ACHKW5_002641"
     CDS             complement(26973..27665)
                     /locus_tag="ACHKW5_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO7512012.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHKW5_002642"
     CDS             27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHKW5_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO7512013.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEKFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7214 a         8060 c         7213 g         7208 t
ORIGIN      
        1 gtcccctgca atattttgaa tttgcacgat tttgtaggcc ggataaggcg ctcgtgccgc
       61 atccggcata aacaaagcgc actttgccag aaatccaggc ccgcattaaa agcgggcttt
      121 tttatgtgcc ttaccagacg ggcatttcgt cttgcaggaa gggattatgc aggcgttcat
      181 aaccaagtgt ggataatggt ccgtgacccg gaataaatgt aacgtcatcc cccagtggca
      241 gcaatttatc tttgattgaa gaaatcagtt gattatgatc gccacgcggg aagtcactgc
      301 gccctactcc gcctttgaaa ataacatcgc cagaaatcag cagctttgcc cgatcatcaa
      361 aaaacacgac atgacccggc gtatgcccag ggcaatgtaa cacctgtaaa gtcacattcc
      421 ctatgctgat ggtatcgcct tcgttcagcc aacgatctgg cgtcagcggc tggcactctt
      481 ccagaccaaa catacgactt tgcgcaggca agccttgcag ccagaactca tcttcttttt
      541 ccgggccaaa aaccggcacg ccgtaatgtt gcgccagttc cgccgctgcg ccaacgtggt
      601 ccagatgacc atgcgtcagc aggatctgca tcagtgtcag gccgctgtca tcaacttcct
      661 gtttgatttt ttccgcatcg ccgccaggat cgaccagtgc ggccagacgg gtttgttcac
      721 accagattaa tgaacagttc tgggagaatg cggtgaccgg aataatacga tagttcatac
      781 tgcccctgtt tcgttaagca attactacca gtgccgtgct ggcccggtat caatatgcac
      841 aaagttacta cgtggataat atcctacacc acctgcgcgc atagataacg cggctttgcg
      901 aatattgctt aacgcgatac cttcaatatg gaaatccatc gcctggcctt tagtgtgata
      961 gcttttcttc gctactccac ggctgcgggc gcgtagttca ttgttggtat caatagaacg
     1021 ataaccggaa atgagttgca ccggtttgcg cgtgcctaac aacccttgca ggcgatacaa
     1081 ctggtcgaat aatcctgggt cgatggactt tattttgttc gcgcggtaat cgcggaaaaa
     1141 atggttaagt tttgccaatt cttcctgaat atagcctctg ccatcgaaaa actccgcttt
     1201 gattgactct ccggtatgaa gattattgag tgtcaaaatg cgcgggcgtg gggtagagag
     1261 tgttgcaaac gcaggggtcg gcaggatggc ggcaccgagt gcaacgccac caagcgccag
     1321 caatttgcgg cgattagcgt cgaatttgtc catgataatc aagtctacag gtcaatgtta
     1381 tcgtttatat gcacttctgg cgcacaagag gcaccttaac cgcccgtaaa gcagacgtca
     1441 agatgactca accccagcag tgacgggggc tgcagagaat cgcccccaat catgctaatt
     1501 attacgacaa ctgatttccc cgaactactt catttacctg attaattgtt ccgctttcga
     1561 tacgatttgc gagctggatc gcgcaggcag atcataattg taaatatctg tacgatactg
     1621 ggtacgacca tctgcaccaa caaaggccgt caggtagtag agattcaccg gaatcgactg
     1681 ccgaatattg acgtaacgtg tatcaccctg cttcagcgca tcagaaatac gtttgtcatt
     1741 ccagcctgca tcctgcaaca gcatattcgc cagatcggaa gctttattca ctcgtacaca
     1801 gcctgagctc aatgcgcgtg tatcacgctt gaacagattg tggttcggcg tgtcatgcaa
     1861 ataaatggcc tctgaactcg gcatattgaa tttatagcgc cccagcgagt tccgtgggcc
     1921 tggagcctgc tggaagcgga acggtaaatt cgaggccgtg attgtagacc agtcaacctg
     1981 ccatgggtca atcgcttctc tgctgttcca gccgcgcatc accgtatagc catggctttc
     2041 gagatatccc ggatcgttgc gcacttttgg cagaatatct ttgcgtgcca gagttggagg
     2101 tacgttccac ggcgggttta ccactacgtt gttaagggca ctgctcatca tcggcgtttt
     2161 gcgatcgggg cgaccgacaa tgactcgcga atccagcacc tgattgccgt tctgatagta
     2221 gaccagcgaa taggccggaa tgttaaccat gatcccggta gaaagctctg ttggcagcaa
     2281 gcgcaatcgc tggatgttga gagccaacac gccagcacgc tgggcgggcg ttacgtttaa
     2341 ccagtcacgt gttgccgggc caatagcacc atctgccccc aatccttgcc atgcctgaaa
     2401 acgtttaacg gcttccacca gttcattatc gtaggcggcg cgaacggcag gcgcaggttt
     2461 actacgagat gtcgtttgct tgtccatcgg cttagtttct gctgtttcaa cagtaacagc
     2521 ggatgggctg actaccgcgt cagttggcgt gtcatcgcca ggtagagtaa ttttcggccc
     2581 cccgtccaac atgcctgtgc gttgcaatat ttcgcgcaac gccggtacgt cgttactcca
     2641 ctgccctggg cgcaacgttg ctttgccggt cagttggggc cacggtttgg tgtcactgag
     2701 taaggccagt aacgattcat gcatcgccgc atattgcgga tgctgcggtg ccagtccagc
     2761 aacaaacgta ggcaattgac ctttatccag cgccagctgc cattggttaa tcaccgagag
     2821 cggcggcgtt gcaagtgcat aaggtttact gctatatagc cagcgagtgc ctttgaccgg
     2881 aatatttgca atgaaatgga gatagcccat catcgcatca gagagcacta cgtcgcgtgc
     2941 catcccgtta acaccaggat cggtcagtaa ctctacccat ttattaaact gcggctggaa
     3001 accggcaatc gccacctctg ccagctgttg ctggaaggct ttaacagcat cacggttttc
     3061 ccacatgggt tgcatatcgc gtgcggcata caacagttga agctggttaa gataaaccgg
     3121 cttgtaacct gcgggtaatt gagattcgat ttgcgtgcgg gctttttctg ccgcaccttc
     3181 aggcaatggc tggatccccg ccattattgc cgtggcttgc gcctgcggca gtgcctcgcc
     3241 ctgttcactg acagccaccg ggctgtcgcc agggattact tcaggctcat cggcctgcgc
     3301 attgaacagt ggagcgaatg ttacggccag gcacaaactg attgccgaca gccgacgacc
     3361 acacatcata ttaagcaaca tcccttgccc cctgttttta tcatactctg aatagccagg
     3421 ctgttatttt acgacttgcc tgtacaacgc caatactcac gaaagtatga cgagcaagcc
     3481 tgttactcat caggcttatc ttcagtatat aaacgccgat acgtttttac ataacctaat
     3541 gtaaagaagt ttaaagaaaa gcgcgccgta atgtgccact tttttagtgc agaaacggag
     3601 ttttcggaaa aagaaaaggc ggcattgctg ccgccttaat tttaactcgc ctgagaaggc
     3661 gcttcgtcac ttcctggaag cgtttcaggg agttgcggcg caaatcctcg caggccgacg
     3721 acgtgaacgt gttcggtatt ctggaagact ttacgcacca gtttataggt ggtgcctttc
     3781 tccgggctga tattttccgg cgctgcgatg atgagctgca tttgcaaacg ttcacacaat
     3841 tcaaacagcg tggcgataga acgagcatcc agtcgcgctg cttcatcgag gaacagcagg
     3901 cggcaaggag agatatcttt accgcgcagg cggcgagatt catcttccca gctttgtacc
     3961 accatcacca gaatcgacat accggtacca atcgcctcac cggtcgacaa tgcaccagac
     4021 tctgcgcgca accagccatc ggaaccacgg ttaacctcaa cttccatttc cagatagttg
     4081 cggtaatcca gcagttcttc gccaatggtc tgcggcgcgc gctgccccat atcaatctgc
     4141 ggattaagac gctgatacaa tttcgccagc gcttccgaga aggtcagacg gttgctgtta
     4201 aacagatcct gatgctgctc gtgctgttca gagagcacat ccagcagcat ggcgtgcgtt
     4261 tcacgcacgt tcacgttcag acgtacgctg ttcacctgac caaacgatac gttctgcaac
     4321 ccctggttga gcatacggat acggttctgc tcgcgctgaa tggttttgcg aatgatgttc
     4381 gccacgctgc gggaactgat cgccagtttc tgttcgcggg aggttaactc ctcggtcagt
     4441 cggctaagtt cgatttccat ctgctcgatg gcttccaccg gatcatcggt acgaataata
     4501 tcctgacgaa tacgttcacg cagatgctga taaaccgcca cgaagaactg aattttacgt
     4561 tccggacgtt ttggatcttc cgacatgcgc agcacgtcgc gcagatgttc gttatccgcc
     4621 accgccagac gcagcgcacc caatgcctta tccgacatag agcgcaaatc atcagcggag
     4681 agataagcca gctcgcgacg gtgtaagcgc cgttcaacgc cgttatcttt caccatgcgc
     4741 atcactgcac accagcccgc tttggcggtc actacctgct cgcgcatctc aaagtaatcc
     4801 cgctccagct tgcgcagttt gcgggtcaga ttgtccatct ccgcttcgca gaaagtcagt
     4861 gctttttcca gctggttgcg gcgggaccgg ttattactca gttgcgcatg cagctcgtca
     4921 cggcgaattc gcgcccgctc ttctgccccg ctatcggcgc gcacgccgat atcctgcaat
     4981 tcacgttgca gatcgttgag tagctctttt ttggtgtcgt aagaactttt cagcgaagcc
     5041 agcacctggt tgtactgact caactgcgct gcgtgaccac gcaacgcttc acgagcgcga
     5101 gtacgctccg cttccgcctg ttccagacgt tcacgcagct tttcgttgag atcgctatta
     5161 ccgctcagca tttctgccga gtcagaatag ctaaagtgcg cacgacgctg caccacttcc
     5221 gtcagggcaa acgcctgctg acgggcatcg cgctgcatct gctgagagta cgcgtaatct
     5281 tcttttagct gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat cggttccagt
     5341 ttcgccagtt gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc ttcatccaga
     5401 cgttcgcgga tttcatcgac gcgatccgcc aggctgtcat cagccaacag gttgagacgc
     5461 ggcagaatgc ggttcagcgc cgtaacgcct tctttcgcct gctcaaactg aatacgctgc
     5521 tgctggttat cattttcatg attactcaac gcccgctcca gttcgacgcg acggctgttc
     5581 agttgacgga tttctgcttc cgggtcagac tcaaacgcaa ccgccagatg actgccgata
     5641 aagcggctga acgcctgatg cagacgctga gttttctgta catcaaagga gagcgtggcg
     5701 aagcgttcgg aaagcacttc acgctcggca tggaggcttt caatacggct ttcacgcgca
     5761 gcacgaccaa acagcggcac ttccgggaaa cgtgaataac gccactgacg atcggcgatt
     5821 ttaaccacta ccgctttttc cagctcatca acgctgaaca cgctgtcatc gaatgactgc
     5881 ggatcccctt cgatcaaata gagatcttcc gggcaatcgg tcaagccttc caggtgttca
     5941 gttacctgtg acagatctgg caccacgatg gcgtggcgtg acgggccata cagcgctgag
     6001 aagtacggcg catcttccag gctaacgtcg tcataaattt ctgacagcag cacgccgcca
     6061 aaacgctccg ccagcgcgtt cagacgctga tcttcagagc cgccaggctg gcttaaacgt
     6121 tcgatctctt catcgacagc gtttttgcgc gcgcccactt catcgcgttc aacaatcgcc
     6181 tctcgctcac gctccagcaa ctgttgcaga tattcggtga cgtcctggct ggaggtaaac
     6241 tcttcaccgc actgctcgct caactggttg agactgtttt gcgctgccag ccaaaccggc
     6301 gcacgctgca tcaaactctg aatgcgagac tgcagctgtt cctgttcctg gcgcagtgcc
     6361 atgcgctctt cacgggcgtt agacacgcta tcggaaagag aggcaatgcg cgcttccagc
     6421 tcctgatgca gagcttccag ttcatcaata tcaaaattct tgccctgacg tttgcagaag
     6481 tctgccagca ggcgttcggc ttcttgctgc tcgcgcagac gctgttccag ttcacttaag
     6541 cgcatccgca acggttgaac ctgctctgcc aggtgacgct gatcgacccc ttcgcgcagc
     6601 agttcgcggg cgacatccca agcctcgtta cgcgccagcg ggccgttgat tgccaccacc
     6661 agctgataag cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat tttctgctca
     6721 agagagagca ttttttcagt cgcttccagc tctttcgcct ggaaggtttc cagccattcg
     6781 gcggcgctgt cggcggttaa gtccggcaga tggcacagtt ctttggcgcg attaagcgca
     6841 gcaatcgcct ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc ctgctggtag
     6901 tcagcaagct ggcttttcag ctcgtccact tccagttcgg cagcttccgc acgagcctcg
     6961 ttctcttcct ggcgttcgat ggcttctgcc accacttcgt tttgctcttc cagacggatc
     7021 tgcagctcat cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg cagtgcggtt
     7081 tgcaccaggt tcaggtgatc gctggccgcc tgataatccg cttccagatc gccttcggca
     7141 ccgttgtgct ctgccagctc acgcgccata tcgacgtgtt tgtactgctc agccgccagt
     7201 tgctgacgcg aagtatgtag ctcgcgacga aactccaggg ctttgtcgag atggacacgg
     7261 cgctcgttgg cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc gctgatcaga
     7321 tgcttaaaca ggtcacggtc cgactgggtg acacgaatcg cttccagcgt catgcggttt
     7381 tcacgcagcg ccgcttccat gtcctggaac gctttacgca cgccgctgtt ttccggcaac
     7441 aggtagtcgc gcagagaacg ggtaatggca ctggagatcc cgccatacag cgaagcttcg
     7501 atcagacgat agaatttgct acggtcagat gcggagcgca gacgacgcgc gatgatgccc
     7561 agatcgaaca tcagcgagtg gtaatcggta atggagttga actgtttaaa ctgcacgcct
     7621 tccatcgcct cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc ctggcgttcg
     7681 ttcagggttt cggtcaccag ctgcgtcggc agcaccgaca tcggcagtcc ctgaatggca
     7741 aatggtttga tatccacttt acgatcgcgt ccggcaacct gttgcagacg cacaccgacc
     7801 accacgcgct ggtggcgcga gttaatggtg tcgagcatcg aataacagac acccgctttc
     7861 agcttaccgt gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc cgtagtgtta
     7921 cggaaatgca gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc catagtggtg
     7981 gatttacccg ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag gtcaaaagtt
     8041 cgggcaaaaa agccgttcca gttaatcagc gtcagtgagc gaaatttacc gcgttcaatc
     8101 attattcttc ctctccgcta tctggctgat tctcttcgtt ttcatcgttg agttgcagat
     8161 gattttcaat cggcattgct tcgccatcac gaatcaggcg acgctgtgct tcacggggat
     8221 cgtcgccagc acgcacatcg gctccgaagc ggaacaccga ttcggtaatg cggaacttgc
     8281 tgctgtcgtg gcccataaac cacaccatgc ctaaacgacg cagacggttg agcgaagaac
     8341 gtactttctc ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg ttgttcacca
     8401 gtttcagcag tttcgcttca tcggccaggg tgagcagttc gtcgtacagt tcctgctggg
     8461 tgaaaatccc ctcattcgcc agccgttccg gactgagata gagataacag aggattttcc
     8521 cgaccatcat atccagttcc gacaagacgg aacgagggat cagcgtggtg gaacgtgggc
     8581 gtaaatagaa gaccccttct ggtgcgcgaa taagctcaac gttataacgc gcgtaaaact
     8641 cttccagata ttcctgaaaa tccatcagga atgcatgatt atccagttcg tcgaggccaa
     8701 tatggcggcc tgaacgtaag gcgctgtcca gcgccggaaa taacggattc gccagcgcct
     8761 gcgccagctt aaccggcatc acttgttcaa tatttgtcga tgacatgcgc ctgtaccttg
     8821 gctccgtaat cattaatcgg ctgccatttc gctggcagcc cggtgaaatc tgcttgcgct
     8881 acgccaagac gtaccgcctg atcaataaca atgcgcgcaa cgtcaaagtg acgtgcacgc
     8941 ggatactgtg acagatattc gcgtaccacc agaccaagat ccagcggcac ttgtctggtt
     9001 ttgtacacag caagttgttc ttcgatgatc gccgccagct gttcgcggat ctcgttaaac
     9061 tcttcgtatt ctaaatctgg cggaagttcc ccagtcactt cttcatcgcg cagtgccatc
     9121 tcttcgtcac gcatatccag cagacgatcg gcattggcat aagttagcgc ccacggctca
     9181 tcaaaatagg tttgtaccga ctgacgtaaa cgctgggcaa agacgcggtt tttatccata
     9241 tcgatcgcag tacgaataaa tttgtgtacg tggcgatcgt agccaatcca caagtcgatg
     9301 gattgctggc cccagctgat aatacggtcg agtttgctct gcagatcgaa caccagacga
     9361 tcgacaaaat gcaggtcgtc atgggtcatc gtcgcatcct gaatgcgcaa cagattagcc
     9421 tgcaacttgt cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt tccggaagtt
     9481 tctgaaagca acaattcaca gctggaaatc gccgcccgcc agtctttgtt cagcaactgg
     9541 gcaatatcgt ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt caggtcgata
     9601 ctgtcgaaaa tttctgctac cgaatatttc agtggcgcat agacattacg gtgccagtga
     9661 aattcatcac cgccctcttc ggcggcatca gctgcgcgtt tgagttcacc cgccacaatc
     9721 gacaactgca tagaaagacg cagcgtagaa aactcgcgct gacggatgta gtagtcagta
     9781 atgccgatgc cgagcggcgt cagacggtaa attgcgttcc cttccgcctg ctcgctggta
     9841 aagcggttca gcagacgttg acgcaccatg tcgttgatcg cgttattggc gcgcacgccg
     9901 atggtttcgc tggtttgctc aaacgcatca ctcacatggc ggaatgcatc caccagctcg
     9961 ccttcactca tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc cagcagaaaa
    10021 gagagtcggt ctaccggcag cgagatggag aagtcatttt ttctggccca ggcaaccagt
    10081 tcggggactg tctgggaaaa ttcactcata ctttatcctt gctctgcggt ttgcgcgcgg
    10141 tgacatgaat ataacgcccc agggtaatat acggttcctg acggcaataa cgcgtttcta
    10201 attcaagtaa tgcttcatag cagtcgcgct gctggtgctt ctcgcgcaga taatcatgaa
    10261 acacgcgaac gcctgtctta cccataattt gccaaccagc ttcttccagc cacagataaa
    10321 cctgcgccgg gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt ttcggcattc
    10381 ccgcctgcac gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa ccatgcgcat
    10441 tgtagaacat taacgacaac acgccgcctg gacgcaacac tgaccacagg gtctgcaata
    10501 cactgcgggg atcggccacc cactcgagca ccgcatggaa caatatcaga tcaacgggcg
    10561 tttccaaatg cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg ttgtcgctca
    10621 caccttttgc ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa agatcgcata
    10681 aaatgacctg atgcccacgc tcggccattt tgattgcggt ctgcccttct ccaccgccag
    10741 catccagcac acgcagtttt tgcgggccca tttccgccag cacgcgatcg agatcctgcc
    10801 acagaatagc ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg gaaaactttt
    10861 ccgcaatatc atcaaaattg cgatcctgca ttgctttctc caccggttgg ctaagacgtt
    10921 ttctgataaa gacgctattt tgtcacagcc acggcgagaa tgaacccgtc tggtgtaata
    10981 tcgctggcga tcccttgcta tatggttaaa aaaggaacca gaaaggatgc tttttacact
    11041 gaaaaaagtg attggcaata tgttgctgcc acttccgctg atgttgttaa ttattggcgc
    11101 tggcctggcg ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt ttattagcat
    11161 tgggtggctg gcactcttgt tattgagttt gcaaccggta gccgaccgcc tgctgcgtcc
    11221 catcgaaagc acctatccga catggaataa ttcgcaaaaa gtggactata tcgtggtgct
    11281 cggaggtggt tatacctgga acccgcagtg ggcaccgagc tctaatttaa tcaataacag
    11341 tctgccacgc ctgaatgaag gtattcgctt atggcgcgaa aatccgggat caaaactgat
    11401 cttcacagga ggcgtagcaa aaaccaatac ggtgagtaca gcggaagtag gtgccagagt
    11461 tgcgcaatcg ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc caaaagatac
    11521 cgaagaagaa gctgcggcag tgaaacaggc gattggtgac gcccctttcc tgctggtgac
    11581 ctccgcatca catctgccgc gggcgatgat cttttttcag caggaagggt taaatccgct
    11641 accagccccg gcgaatcagt tggccatcga ctcgccgctt aacccgtggg aacgggcaat
    11701 cccttctccg gtctggctga tgcatagcga tcgtgttggc tatgaaacgc ttggccgcat
    11761 ctggcagtgg ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt gatgccagat
    11821 cgaagttgcg ccgactaaat cgtccggtat taaccagttg cgcaacttca tcccataaca
    11881 catacaacca acgccgccag atgaacgact ccgccactgg cgcgcgttgc aggtaactcc
    11941 agagcaaatc ttccgccaga gaattatcca ttagtcggaa cagttcgtat tctcgcggtg
    12001 cccaaagcat tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc gaatctttca
    12061 gcatgctgcg taaacagaag ttaccgtgaa tcaacacaca attgtcgtta aagccttcaa
    12121 ataatgccgg gagacattcg cgagtgcgaa acaggatccg cttatcctgc atcgtcagac
    12181 cggtattatt gaactgattg agcgtggtcc atagcacttc aacatgttgc aggtaccatg
    12241 agggccagaa attttcctga gtattgtcga ccgcgccgac gcaaccgcga ctgtcctgac
    12301 ggtgccaggc cagtaaggct tcaacgattt ggtctttgag ctgttcccag cgttctggtg
    12361 ttcgggctgg tgcctccacc gaaacaccac gcatccgctc cagtagcagg acatcagggc
    12421 cggggtgttc ttcatgcgtc atcacaccat aaatagtcgg catacggaca gtcccacttc
    12481 ttgccagcat ggtggttttc catgccagtt gtcgggcttt tccgggcgta ctaaagcttc
    12541 ttgccattaa cggcattggg tttccctggc tgtcatacaa agcccacaac gccgtatccg
    12601 ctttttcatt gacgcactca atacgactga gtttttcgcc cagtaaatgg cttaattcgg
    12661 cacgcagctg ttccattcag attaccctca taaaagctac tgctttcata atgagcgcca
    12721 atataaaaga tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga ggaaaatcgt
    12781 aacggtacga cactcctccc aaaattggct gaagtgtcgt gaagtgaaat tagcgcattt
    12841 cagcgcgaac gcgctcaaga tcttcagggg tatccacacc tgtgccagga acttcctgag
    12901 caacagcaac atggattttt tcgccgtacc acagaacacg aagctgctct aacatttcga
    12961 tgtgttctaa cgggcttggc tgccaggtga cgtaacgacg gataaagcct gcacggtagc
    13021 cataaatacc aagatgacgc aggaagttat cgccaacggt ttcaaggtct tttgcaaaac
    13081 ggtcacgatc ccacggaatg gtggcgcgtg agaagtacag agcataccct tcagcgtcga
    13141 gaacgacttt caccgcattc gggttaaacg cttcttccgc attgtggatt ggcgccgcca
    13201 gagtcgccat acccacctga cgctgagcga ggttatcagc aacctgacga atgatggtcg
    13261 cagggatcat cggttcatca ccctgcacat taacgatcac cgtgtcgtcg ctgaatgcgc
    13321 atttttcgac aacttccgcc agacgttctg ttcctgactg atgatcggcg cgcgtcatac
    13381 atacttcacc gccagcggct tcaacggcgc gggcaacatc ctcatgatcg gttgccacga
    13441 tgatgcgctc ggcacctgat tcacgcgcgc gttcaagaac atgaacaatc atgggtttgc
    13501 cgttaatatc aaccaatggt ttaccgggca gacgcgtgga cgcgtagcgc gcgggaataa
    13561 tgaccacaaa actcatgatt tactctcatc agcagtcagc acgcgggctt ccgtttccag
    13621 caacactgga atgccatcgc ggagcgggaa agccaggtta tccagtttgc aaataagctc
    13681 ttgtttttct tggttatacc aaagttttcc gttgcaaacc gggcaggcaa tgatttcaag
    13741 caaacgatga tccatagttc ctccagatgg atcgggttat gaatgcataa atcttatcat
    13801 aatcatttat gcgacggggt ctatttccca gccagtgcgg caatcagtaa agagaccttg
    13861 cgggcagcgt cctaatgtca cccgcgttgc ctgctgccag ctcgcgaaat cagtaatcgc
    13921 ctgacgtaac cctttttgca gcattgtcgt tggtttaatg ccttcctgta accacagaga
    13981 gataacttca aggatgcctg tctggcgatg cattttggca tccattcgcc caactaattg
    14041 cccacgatgt aataatggca gaacaaaata gccatactgg cgtttcggcg ctggggtata
    14101 gcactccagc cggtagctaa aatcaaaaag ctactctgcg cgtttgcgat cccagacaac
    14161 aggatcaaaa ggcgaaagta ccgcgctgtg cgttgcagtg agctttccgg ccagcgcacg
    14221 ctcgagtagc ggcagcaaat cagcatgcag ccaaagattg cccaattttt caacatgcac
    14281 agcaatgatt tgctgctgtt cagccctcgc ttcgcgccac gccgccagtg ccggacgttt
    14341 cagccgatag taatctgcca gccactgttc gcggaatatt cccaggctac gcgcactgtt
    14401 atccagcatg atgatttctg cttctgtttg cgaaacgaga tcgcgctcat catcccagtc
    14461 aggcatgaca cggtgggtta aatcataaac gcgctggaag ttgcgccgtt caatcaccat
    14521 cacctttccg gcagtaaata aaccttccag atgccgttta tgcggcttcc attcccacca
    14581 gccgcttgca cctttacgag gatgctcaaa atcggctgaa cgtaccggcc ccttatcatg
    14641 aatatgccga attaactgtg caatttccgc ctcatgttcc tgcatccagg cgtctttgta
    14701 tttccagccc attttttcag gtgccagcat gcggtggcga ataagacgaa agtcgctacg
    14761 cggcataaag caggcttcat gcgcccagta ttccattaat tcgccacgcg ccagagactc
    14821 atccagccac tgggcaggat aatttcccag acgactgaaa agcaccagat atggactgcg
    14881 ggcaacaata ttgatggtat cgatttgcag caaggacatg cgggagatcg ttgccggaat
    14941 atcctccaac gacgcttgac ggcggggttt gtttaacagg ccttgtgcgg caaggtgaag
    15001 attacgcgca tcagcaaggg agaggtgcgg cagcgacatt catgactcca tcaatcgaac
    15061 gctgccgcgg cgtaactagt tgccagaagc cagcgaggtt agttgcgtaa gcagtttcgc
    15121 tggttcatca cctgaaagct gtgcgtctac aggcaaatac caccaatttt cttctgcaaa
    15181 ggcccggcat ttcaccgcat ctttttcagt cattaccagc gtttgcccgg cgcttaccaa
    15241 cgcactgaca tccgcatggt tcaaagactg atgatcggcc agcggtacac atttttccgg
    15301 ttgtacgcca cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa tccccgccat
    15361 cgccactaca tgttcaagct gagcaacgtc acaacgcgta ccggtacgta aattcaccgc
    15421 ctgacccggc agcagatgca tggggatttc accgctgcga gggacaccgc cgttgacgat
    15481 taccgcatca accgacttta agcgccccgc tcgctcacgc attggccccg ccggcaacca
    15541 ccagccattg ccaaagcgac gcacaccatc aataacgaca atttccacat cacgcgccag
    15601 acggtaatgc tgtaaaccgt cgtcggttac gatgatctgc acatcagggt gttgcgccag
    15661 aatggctttt accgcatcag aacgaacggg agaaaccgca acaggcgcat cagtgcgttg
    15721 ataaatcaac acaggttcat cacccgcctg tgctgttgtg gtatctgccg acaataacag
    15781 cggataagat tcagccttac cactatatcc ccgcgatacg acccccacgc gaataccgcg
    15841 ctgttgcaac tgttccacca gccagacaac gaccggggtt tttccgttgc cgcctgcggt
    15901 gagattacca accacgacaa ccggtacggg ggcacgccag gcgcgcttca gttttagttt
    15961 atagcaaaga cggatcgcgc cacttaccag gccatacaac caggagagtg gcagcaatag
    16021 ccgccacaaa ggggattcac cagaccagat tttttcgatc attggccaaa ctgcattttg
    16081 tgaagttgcg cgtaaacgcc acggtgctca agtaaatcgt tatgcgtacc gcgttccaca
    16141 atgacaccat cctcgacgac cacgatttcg tctgcctttt caatggtaga caagcggtgg
    16201 gcaatcacca gagaggtacg gtttttctgc aactcatcca gtgccgcctg aatcgcacgt
    16261 tcggattcgg tatccagagc cgaggtagct tcgtccagaa tcagaatcgg gctatcacgc
    16321 aacaaggctc gagcgatagc aatacgctga cgctgaccgc cagagagcag cacgccgttt
    16381 tcaccaatca ctgtatcgag accgttatcc atcttattga tgaagtccat agcgtaggcc
    16441 atacgcgccg cttcttcaat ttgctcacgg ctgtactgtt cagttcgtgc gtaagcaatg
    16501 ttgttagcaa ccgtatcgtt aaacagatgg acattctgcg acaccagagc aacctggtta
    16561 cgcaacgacg ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat ttcgccttca
    16621 tcaatatcgt aaaaacgcgt gatcaggctg gcgatggttg atttacctga accagagcgt
    16681 ccaaccagag caaccgtctt ccctgccgga attttcaggt tgatgttacg caatgcaggt
    16741 acgtcacgtc ccggataagt aaaggtgacg ttgcggaatt ccacgtcacc tgtcgcacgc
    16801 tcgatcacgc gcttaccttc gtctttctcc tgctcactgt ccagaatggt aaacagcgtc
    16861 tgacaagccg ccataccgcg ctggaactgg gcgttaacgt tggtcagcga tttcagcgga
    16921 cgcataagtg caatcattga agagaaaaca acggtaatcg taccggcagt caggctatcc
    16981 atgacacttg ggaagcttgc cgcatacaga acaaacgcca gcgccaaaga ggcgatcagc
    17041 tgaatgatcg gatcagagat ggaagaggct gaaaccattt tcatcccctg aagacgcatt
    17101 cggttgctga ctttatcgaa gcgtttcgtt tccacttcct gaccaccgaa aatcaatact
    17161 tctttgtggc cctttagcat ttgttcggcg ctggtggtca cctgccccat ggtgttctgc
    17221 atgtttttac tgatgttgcg aaaacgcttc gatacaacgc gaatcgcaat cgaaacaatc
    17281 ggtgccagca caatcaaaat gatcgacagt tgccaactgt aatagaacat catgatgaac
    17341 aggccgatga ttgacgcacc ttcacgcaca accgttatca gtgcgccgga agaagaagaa
    17401 gcaacctgtt cggaatcgta ggtgatacgt gacaacagcg tacccgttga ctgtttgtca
    17461 aaaaaggaaa ccggcattcc catcatgtga tcaaacaggc ggcgacgcat ggtcattacc
    17521 acctttcctg atacccagga gatacagtag ctggagacat agctggtgat accacgtaaa
    17581 atcatcagcc cgatcaccac cagcggcatc cacaccagca cggagcgatc tgttttacca
    17641 aagccatcat caagaagtgg cttaaggagc gataacatga aggtatcgct ggctgcgttg
    17701 aggattaacg ctacgcccgc cacgatcaga cccgctttaa aaggcgcaat ggttggccac
    17761 agtcggcgga acgtctgcca cgtagagaga tctttgtcgt tatgcattca aaaaaccagc
    17821 atttgttgaa atagccgcat attctacccg ttatccactg gcacgccaaa ccactgatga
    17881 taccaacgag gtaaaatttg ctctctgaga ctgctaatcc gccagccttg cgctgaaaaa
    17941 ttgaccgtta cttgcccctg atgaggagta tcgagccacg tataaccctg ttgttgatag
    18001 cgatgcttaa ctttgttaga gggcagtcgc catgcgttat agcgcgatgc cgatgcgagt
    18061 gccacttttc cattcactcg ctgaattaat ggcaacgatg atgaggtatt actgccatgg
    18121 tgaggtacct gaagcaatgt tgcctgcact tgctgccagt aacggcttag catcttttgt
    18181 tcagcgggga cttcaatatc accggttaga agaatgctat tcatcccgtc atcaacctta
    18241 accacacagg aatggttatt tcctttatcg ttgctagctt gtaaaggcca gtgcacgctg
    18301 aaacgcaatc cttgccattg ccacgcttcg ccacgcacac agggctgatg atgttcccag
    18361 tttaacggac ttctgatcca taacatcggc catgtatgca atattgagtc cagccctccc
    18421 cggtgatcca gatgttcatg actcagaata acgccttccg gttcaagatt atgccagtgg
    18481 agccagggga taatcagttg ttgcccacta tccccttcag gccaggccag tcccgtgtca
    18541 tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc gacatcaagc
    18601 atgtacactt gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagtaag
    18661 cctccagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt
    18721 aagaaccatg gggaaaatga tagccattgc caacgttcag caatgttgat ccacccttcc
    18781 ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac
    18841 ccttgctcca ggattaacgg cccgcttaaa tgcacaacca tcgcggcgag gatcaacgga
    18901 accgtgataa atgtcaccaa gggaattgca aatagatttg caataaacga ggtcagacta
    18961 atgccatgaa atatgacgat ttgcacgggc ataagcagga gtgtgattcc cagttgcaaa
    19021 tgaatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac
    19081 cactggtacc aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag
    19141 agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag
    19201 ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta
    19261 cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagattctg
    19321 ccaattaatg gtatttgcca gtggatccag cgcccaggca gaaaaatttg tccactgcga
    19381 atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa
    19441 tgcgccgttc cagtatcacg catgatattt ttgatttctt tagggacgga taaccgtacc
    19501 cccataccta aaccaagaat aaccgcattc cacatatagg gttgcagcgt tgtttgtagt
    19561 gacgccagat actgtgcccg caggctacaa ttcggttcaa tgacacttgc ctgcagaaag
    19621 cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca
    19681 ttaagttgac catgaatggc acgaactttg agtttcattg accacagttg tccagcacaa
    19741 accgcttgcg gaagatattc gccatacagc acgaggcctg gcgcagggaa tatacgtcga
    19801 ccttgtagat gagtgatttg accgtaatga gtggtcatgc cgtcagtagt agtgatctca
    19861 acaattgcat cctgcgtcgc accagttaag gtttctcctg cccacaaaat ttgctttgcc
    19921 gctaatatac cccaaacgaa aaacagcaac gtcagcgcga tataacgtac ggttttaaca
    19981 gggataaatg ccagtacgca ggcgaagata gtcagaaacg caagggttaa tgtcccaggc
    20041 aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt
    20101 attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg
    20161 tgggaatttt gcgttccccg aggcacgttc agaagaattt aacgagaggg tgaaaactgc
    20221 tcaaaactct gcgaagcagc ttccagttcc agattagaga aatgaaaaaa gcacccgaca
    20281 ggtgcttttc tctcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc
    20341 gcagttcttt accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc
    20401 cagtcttcgg attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac
    20461 cgcggatttc aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt
    20521 ttactgcatc ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta
    20581 tcaattctga cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt
    20641 tgctgcattg attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct
    20701 gcaatctgtc aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt
    20761 actcgccttt agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt
    20821 cctgtttgtt aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag
    20881 acaggctgat tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc
    20941 caacgctcag aaccagggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca
    21001 ggtaaccttc aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta
    21061 ctttaccggt tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt
    21121 ctgcgagctg tttaacgccc agggagatac gttcacgttc tgcgtcaacc tgcagaacaa
    21181 ctgcagcgat ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc
    21241 aggagatgtc agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac
    21301 cgaagtcagt gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg
    21361 cgaactgctg ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt
    21421 cttcgtcgat atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg
    21481 ggtggatgtt tttgttggtc caatccattt cggaaacgtg taccaggcct tcaacgcctt
    21541 cttcgatttc aacgaagcag ccgtagtcgg tcaggttggt cacgcgacca gtcagtttgg
    21601 taccttccgg ataacgttta gcgatagcta cccacggatc ttcgcccagc tgtttcaggc
    21661 ccagggatac acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc
    21721 ccacgttgac gatttcgctc ggatgcttaa cgcgtttcca ggccatgtca gtgatgtgca
    21781 gcaggccgtc aacgccgccc agatcaacga atgcaccgta gtcagtgagg ttcttaacga
    21841 tacctttaac ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt
    21901 tttcggattc gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct
    21961 tgattacttt gaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa
    22021 cgtctaccag agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc
    22081 ccttaacttt gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca
    22141 gcgtgatcca ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc
    22201 cgtcttctac tgcgtccaga gcaacgtcaa cttcgtcacc tacctggatt tccagctcgc
    22261 cctgggcgtt tttgaactgc tcagccggga tggcggactc agatttcaga ccagcgtcaa
    22321 ccagtactac gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg
    22381 tttcgatttc ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc
    22441 ttcaggttta tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc
    22501 gctgtcattc cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt
    22561 ctggcgcgcg tattgtagcg ctttttcaat cacttgctca atgcttaagg tggtggaatc
    22621 caacactaaa gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc
    22681 gtcgcgttct ttgatctcgg ccaaaaggcg ctcaaagtta acactaaagc ccttctcctg
    22741 caactgtagc atgcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac
    22801 tggcgcatca gggaatacca ccgttcccat gtcgcggcca tcggcaatca ggcctggtaa
    22861 ttcgcgaaac gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac
    22921 ttgtgaagct gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc
    22981 gaggatcact tccagattgc cattggttga cacaaaacgt acatccaggt gggatgccag
    23041 cggtaccagc gcatcttccg atgcaacatc aacatggtga tgtaatgccg ctagtgccag
    23101 tacgcgataa attgcacccg aatccagcag atgccattgc aatgcttccg ccatagcctt
    23161 acacaaggtg cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc
    23221 cgtcatcttt atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg
    23281 ccgacgccac aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg
    23341 gatttattgc gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt
    23401 acttaccaga ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag
    23461 agtcaaacat ggactgggtg cgaccgccat ccagactagc cagtttttca aagctgccaa
    23521 ccagcccctg ggtgctgatg ccacgtttct tcaacagatc aaaggagaaa tcatctgctt
    23581 cggactcttt atcacgggag tatttagcat tgatagcgcc ttctgcgata tcacccaatt
    23641 gtgagcggga aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa
    23701 cgatcgcata agaagctttc atttcagcca gcgagtgccc caacgcgacg tggcccagtt
    23761 catggcccag aacaccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga
    23821 cacgaacaca gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat
    23881 agttgaccgg cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt
    23941 taccgtactt gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct
    24001 gattggcaat cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac
    24061 cggagctgat agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg
    24121 ctgcagaggt cgcaatcgcc agcagtaatt tagtattctt catgtgaatc atccgttgat
    24181 taatcatata taaatattat gtatcaacaa cttacattga caagcgacgc taccattaat
    24241 caaaatgact cacaaggtcc gaaaaaaaac gccgacaatg tgccgacgtt ttgataaaga
    24301 aagatttggc tatttattgc ccgttgttca ttcaggctgc ctggctaatc cgcgccaact
    24361 gctcgaaata atccggaaat gttttggccg tacatttggg atcaagaatc gtcactggtg
    24421 tatctgacaa cgccaccagc gagaaacaca tcgccatccg gtgatcattg tatgtcgcga
    24481 tctcggcaaa gttcagtttt tccggtggag tgatacgaat gtaatcgtgc ccctcttcta
    24541 cttccgcacc gactttacgc agttctgttg ccatcgcaaa caggcgatcg gtttctttaa
    24601 cacgccagtt atagatattg cgcagcgtgg tggtgccttt tgcaaataac gccgccgtgg
    24661 caatggtcat cgccgcatcg ggaatatggt tcatatccat atcaatagcg ttcagttcac
    24721 cacgcgtgca ggaaatataa tcatcgcccc agcaaatggt cgcgcccatt ttttccagca
    24781 catcagcaaa gcgaatatca ccctgcatac tgttacgtcc aataccggtc acttttacag
    24841 tgccgccttt gattgctgct gctgccagaa agtaagaagc cgaagatgca tcgccttcga
    24901 ccaaataagt acccggagac tgataagact gcccgccttt tacgacaaat tgttgatagt
    24961 gctgattttc aatttcaaca ccaaacgtct tcatcagatt gagtgtgatg tcgatataag
    25021 gtttagaaac cagatcgcct ttaatacgaa tcaccgtatc ttccggcgca agaggcgcag
    25081 tcattaacag tgcggtgagg aattggctgg aaacggagcc atcaacgtca acgttgccgc
    25141 cagtaaagcc gccctgtaaa cgcaacggcg gataattttc ttgttccagg taagtgatct
    25201 tcgccccgcc caggcgcagc gcatccacca gatgaccaat cgggcgttct ttcatacgcg
    25261 gctcaccggt cagcacaata tcattgctac ccagacaaag agctgccgcc agcggacgca
    25321 ttgccgttcc ggcgttaccg aggaacaact ccagggcacc ttctgcgtgt aatggaccgc
    25381 cgttaccgat aatttcgcaa cgcgtacgat cggctgaaag cgtatagctt acccctaacg
    25441 ctgttaatgc attcagcata tggcgcacgt catcgctatc cagcagattg gttaatactg
    25501 ttttgccgtg tgctaatgcc gccagcaata aagcgcggtt agaaacgctc ttggaaccgg
    25561 gcagattaat agtgccatcg acacgagcga tgggttgtaa cgtcagggat tccatgaact
    25621 caactctcta caacagaaat aaaaacccca caggctggct gtggggatta agcaaaattt
    25681 cggcattaac cgtgacggcg ttcgaactca accatgaagt ctgtcagcgc tttaacgcct
    25741 tccagcggca tggcgttata aatagaagcg cgcattccgc cgaccacacg gtgacctttc
    25801 agtgcatgaa ggccagcagc aaaagactct tcaaggaaca atttgtcaag cgcactgtcc
    25861 gccaactgga acggcacgtt catccgcgaa cggttagctt tcgccacgtc attgcggtag
    25921 aaatcgctgt tatcaatcac cccatatagc agttctgctt tttgctgatt gattttatcc
    25981 atttcagcta caccgccgtt cgctttcagc catttaaaga ccagaccaga tagataccag
    26041 gcaaatgtcg gcggcgtgtt aaacatggag ccgttatcgt tgaggatgga ataatccaga
    26101 atcgacggac acgcgatatt cgctttgccc agcaaatctt cacgaacgat gacgattgtc
    26161 aggccagccg ggccgatatt tttctgcgcg ccagcgtaaa tcacgccata acggctgacg
    26221 tcaatcggac gggaaagaat ggttgaagag aagtcggcgg cgaccaccac atctttgccg
    26281 aagtctggcg tttcgtcgat ggcgataccg tcgatggttt cattcgggca gtaatgcata
    26341 taagcagcat tatcagagag ttgccattca cgcattggct taaccgcgcg cagaccatca
    26401 acagtcactt tggcgtcaaa gacattaggc gtgcagtatt ttttcgcttc tttaatggca
    26461 cttgccgccc agtaaccggc atcaacataa tctgcggtgg ttttatcacc gagaatattc
    26521 agcggtaccg cagcaaactg accgcgacca ccgccatggc agaataatac cttgtagttg
    26581 gaggggacat taagaagatc gcgaaaatcc ttctcggctt cctctgcaac ctgaatgaac
    26641 tctttgccac ggtgactcac ttccatcacc gacgtaccaa gaccgttcca gtcgcgcagt
    26701 tcctgttgag cctgtttaag cacctctgcc ggtagcattg ccggaccaga actaaaattg
    26761 aagatttgag ccatttcccc tcaccacgtt gcgttgctat taacccgcgg tcaatcgatt
    26821 gcgaccacag gtaatacatt attcctgtgg ctatcggttt tatcattcag tgacacacat
    26881 cgcaatgagt aaaaccgttg agggcaaaaa tgtggcttgc atcacacaaa agaatctctc
    26941 ggattattag caaaaagccg atgtttttac tgtcaggtca cccgttttgc ccgacttgcc
    27001 ttcgtccatt ctggatttgc acagcgcgga cataattgtt tttcccccgc tttcatatga
    27061 atgatttcac tacaacgaat acaggcatag tccgccgact ccggcaccac tttgtaacgc
    27121 tgcgtacaat cactgggtaa aataagtaaa cccggtttca ccttgtcatc ttcaaagaaa
    27181 tagacactga tttgcccgtt ggtttcgaga atcgccagac gtacctgccc cagctgctcc
    27241 acgccacgca atcgtagctc cataaagaac tcaaattccg tcatgttgga gttattgagt
    27301 ttcgaccagg ccagctcgcc atcttcaata ataacaaccg gcttaccttc cagtagatct
    27361 tccagttttt cactgtgcgc catcaaccac attaccaggc ggtataacaa cgccagagtg
    27421 ataaagacga taagtaccgg caccatcggc acatcatcat aaaacgccac atctcccgcc
    27481 gctgaaccca gcgtgagaat gattaacact tcaaacagcg acatctgtcg cacaccgcgt
    27541 cttccggtca ttttgaggaa caaaaaaacc agtacaaagg tataaagact acgtagtgca
    27601 acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca tacggtggag atcgaacgcc
    27661 ttcattttta tgctcttatc gtggtttatc cgctaaaagc atagtcaaca gttcatcggc
    27721 atggcggcgg tgatagcgcc tttgagataa aacccgtatt attgcgcgct ttccgtacga
    27781 ctaaagtgat tttcatgacg caaacattta tccccggcaa agatgccgct ctggaagatt
    27841 ccatcgctcg cttccagcaa aaactttcag acctcggctt tcagattgaa gaggcctcct
    27901 ggctgaatcc tgtgcctaac gtctggtctg tacatattcg cgacaaagag tgcgcactgt
    27961 gttttaccaa cggaaaaggc gcaaccaaaa aagcggcgct ggcttctgca cttggtgaat
    28021 atttcgagcg tctctcaacc aactactttt ttgctgattt ctggctgggc gaaaccatcg
    28081 ccaacggtcc gttcgtgcat tatcccaacg aaaaatggtt cccactgacc gaaaatgacg
    28141 atgtgccaga aggactactc gatgaccgtc tgcgcgcgtt ttatgatccg gagaatgaac
    28201 tgaccggcag catgctgatt gacctacaat ccggtaacga agatcgtggt atttgcggcc
    28261 tgccgtttac gcgtcagtcc gacaatcaga ccgtttatat tccgatgaat atcattggta
    28321 acctgtacgt ctccaacggt atgtccgcag gtaatacccg caacgaagca cgcgttcagg
    28381 gattgtctga agttttcgaa cgctacgtga aaaaccgcat tattgctgaa agcatcagcc
    28441 tgccagagat cccggcagac gtgctggcgc gttacccagc agtagttgaa gccatcgaaa
    28501 cgctggaagc ggagggtttc ccaatcttcg cttatgacgg ttcgcttggc ggccagtatc
    28561 cggtgatttg cgtggtactg ttcaatcctg ctaacggtac ctgctttgcc tctttcggtg
    28621 cgcatcctga ttttggcgta gcactggaac gtaccgtgac cgagctgctg caaggtcgtg
    28681 gcctgaaaga tttggatgtg tttactccgc caaccttcga tgatgaagaa gtcgctgaac
    28741 ataccaacct cgaaacgcac tttatcgatt ccagcggttt aatctcctgg gacctgttca
    28801 agcaggatgc cgattatccg tttgtggact ggaatttctc cggcaccacg gaagaaaagt
    28861 tcgccacgct gatggctatc ttcaaaaagg aagataaaga agtttatatt gccgattacg
    28921 agcatctggg tgtctacgct tgccgcatta tcgtgcctgg catgtccgat atttatccgg
    28981 ctgaagatct gtggctcgcg aataacagta tgggcagcca tttacgtgaa acgattcttt
    29041 cgctaccagg cagcgagtgg gaaaaagaag attacctgaa cctcatcgag caactggatg
    29101 aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt gggtctggcg accgggtcgg
    29161 ataacggttg gtacaccctg cgtatcggtg aattaaaagc catgctggcg ctggctggtg
    29221 gcgatctgga acaggctctg gtctggaccg aatggacgat ggagtttaac tcatcagtat
    29281 ttagtccgga acgcgccaac tattatcgct gcctgcaaac gttgttatta ctggcacagg
    29341 aagaagatcg ccagccgctg caatatctga atgcgtttgt tcgcatgtac ggcgcagatg
    29401 ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc gttttacggc ctgcaaccgg
    29461 tagatagcga tctgcacgcg tttgctgcac atcagtcgtt gttgaaggcc tacgaaaagc
    29521 tgcagcgcgc caaagcagca ttctgggcaa aataaaatca aatagcctac gcaatgtagg
    29581 cttaatgatt agtctgagtt atattacggg gcgttttttt aatgccccgc tttacatata
    29641 tttgcattaa taaaataatt gtaattataa ggttaaatat cggtaatttg tattt
//
LOCUS       DAZFYC010000054        29547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFYC010000054 DAZFYC010000000
VERSION     DAZFYC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.054"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..138
                     /locus_tag="ACHKW5_002643"
     ncRNA           7..138
                     /ncRNA_class="other"
                     /locus_tag="ACHKW5_002643"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(322..999)
                     /gene="narI"
                     /locus_tag="ACHKW5_002644"
     CDS             complement(322..999)
                     /gene="narI"
                     /locus_tag="ACHKW5_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7512014.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(999..1709)
                     /gene="narJ"
                     /locus_tag="ACHKW5_002645"
     CDS             complement(999..1709)
                     /gene="narJ"
                     /locus_tag="ACHKW5_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7512015.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASENLSKEDAHALG
                     IFLRDLTALDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1706..3244)
                     /gene="narH"
                     /locus_tag="ACHKW5_002646"
     CDS             complement(1706..3244)
                     /gene="narH"
                     /locus_tag="ACHKW5_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7512016.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKAQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3241..6984)
                     /gene="narG"
                     /locus_tag="ACHKW5_002647"
     CDS             complement(3241..6984)
                     /gene="narG"
                     /locus_tag="ACHKW5_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO7512017.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7500..8891)
                     /gene="narK"
                     /locus_tag="ACHKW5_002648"
     CDS             complement(7500..8891)
                     /gene="narK"
                     /locus_tag="ACHKW5_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO7512018.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9230..11026
                     /gene="narX"
                     /locus_tag="ACHKW5_002649"
     CDS             9230..11026
                     /gene="narX"
                     /locus_tag="ACHKW5_002649"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO7512019.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            11019..11669
                     /gene="narL"
                     /locus_tag="ACHKW5_002650"
     CDS             11019..11669
                     /gene="narL"
                     /locus_tag="ACHKW5_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO7512020.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11670..13064)
                     /gene="ychO"
                     /locus_tag="ACHKW5_002651"
                     /gene_synonym="iatA"
     CDS             complement(11670..13064)
                     /gene="ychO"
                     /locus_tag="ACHKW5_002651"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO7512021.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13249..13602
                     /gene="ychN"
                     /locus_tag="ACHKW5_002652"
     CDS             13249..13602
                     /gene="ychN"
                     /locus_tag="ACHKW5_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO7512022.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTHPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13646..14341)
                     /gene="chaC"
                     /locus_tag="ACHKW5_002653"
     CDS             complement(13646..14341)
                     /gene="chaC"
                     /locus_tag="ACHKW5_002653"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO7512023.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMEPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14499..14729)
                     /gene="chaB"
                     /locus_tag="ACHKW5_002654"
     CDS             complement(14499..14729)
                     /gene="chaB"
                     /locus_tag="ACHKW5_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO7512024.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14999..16099
                     /gene="chaA"
                     /locus_tag="ACHKW5_002655"
     CDS             14999..16099
                     /gene="chaA"
                     /locus_tag="ACHKW5_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO7512025.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16504..16611
                     /locus_tag="ACHKW5_002656"
     CDS             16504..16611
                     /locus_tag="ACHKW5_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7512026.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(16760..17614)
                     /gene="kdsA"
                     /locus_tag="ACHKW5_002657"
     CDS             complement(16760..17614)
                     /gene="kdsA"
                     /locus_tag="ACHKW5_002657"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO7512027.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17650..18459)
                     /gene="sirB1"
                     /locus_tag="ACHKW5_002658"
     CDS             complement(17650..18459)
                     /gene="sirB1"
                     /locus_tag="ACHKW5_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO7512028.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18463..18855)
                     /gene="sirB2"
                     /locus_tag="ACHKW5_002659"
     CDS             complement(18463..18855)
                     /gene="sirB2"
                     /locus_tag="ACHKW5_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO7512029.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18852..19685)
                     /gene="prmC"
                     /locus_tag="ACHKW5_002660"
     CDS             complement(18852..19685)
                     /gene="prmC"
                     /locus_tag="ACHKW5_002660"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7512030.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     THLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19685..20767)
                     /gene="prfA"
                     /locus_tag="ACHKW5_002661"
     CDS             complement(19685..20767)
                     /gene="prfA"
                     /locus_tag="ACHKW5_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO7512031.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20809..22065)
                     /gene="hemA"
                     /locus_tag="ACHKW5_002662"
     CDS             complement(20809..22065)
                     /gene="hemA"
                     /locus_tag="ACHKW5_002662"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO7512032.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22279..22902
                     /gene="lolB"
                     /locus_tag="ACHKW5_002663"
     CDS             22279..22902
                     /gene="lolB"
                     /locus_tag="ACHKW5_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO7512033.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22902..23753
                     /gene="ispE"
                     /locus_tag="ACHKW5_002664"
     CDS             22902..23753
                     /gene="ispE"
                     /locus_tag="ACHKW5_002664"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO7512034.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23904..24851
                     /gene="prs"
                     /locus_tag="ACHKW5_002665"
     CDS             23904..24851
                     /gene="prs"
                     /locus_tag="ACHKW5_002665"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO7512035.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24976..26655
                     /gene="dauA"
                     /locus_tag="ACHKW5_002666"
     CDS             24976..26655
                     /gene="dauA"
                     /locus_tag="ACHKW5_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO7512036.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26710..26988)
                     /gene="ychH"
                     /locus_tag="ACHKW5_002667"
     CDS             complement(26710..26988)
                     /gene="ychH"
                     /locus_tag="ACHKW5_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO7512037.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27266..27850
                     /gene="pth"
                     /locus_tag="ACHKW5_002668"
     CDS             27266..27850
                     /gene="pth"
                     /locus_tag="ACHKW5_002668"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO7512038.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27967..29058
                     /gene="ychF"
                     /locus_tag="ACHKW5_002669"
     CDS             27967..29058
                     /gene="ychF"
                     /locus_tag="ACHKW5_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO7512039.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         7111 a         7804 c         7438 g         7194 t
ORIGIN      
        1 ttaaatattc tgcccaagcg gggaaggatg agaagcttcg accaaggttc gacttgagcg
       61 ccagcgagag agcgttgccg caggcaacga cccgaagggc gaagcgcgca gcgctgagta
      121 atccttcccc caccaccatc gcttccttaa ataatcaaaa cctgttactc cgagctaatt
      181 ttgcccgatg gcaccacgtt taccgggcct acaatatcct cccctgtagg tcggataagc
      241 gaagcgcatc cgacaactca ccccgcaccg tgcccataaa aaaaccccgc cgaagcaggg
      301 tctatgtgaa ctaaaattcg cttagtgacg agcgcgcacc agctggtact tacgtgtcag
      361 atactccacc ggtacgctcc agatgtgtac cagacgcgag aacgggaaca gcaggaataa
      421 cgtcatcccc agcaccaggt gcagacggaa gataaacgcc acaccatcga ggtgttgaga
      481 agcgccaccg tggaaggtca ccaccgactg cgcccagcca accagtttca tcatctcgct
      541 accgtccata tgctgagcgg agaacggaat ggtcagcagg cccagcgcgc actggataac
      601 gagcagcgac aggatcagga tatccgctcc agtagtggtt gcacgcacgc gtgggctgaa
      661 cagacgacgc ttcagcaaca gcacgccgcc aatcagacac agcacgccgc tggcaccacc
      721 agcaaacatg gccattttct gtttcacttc aatcggcagc caggcttcat acatccagtg
      781 cggcgtcaac ataccgaaga agtgaccgac aaaaatcccc agaataccga tatggaacag
      841 gttcgacgcc aggttcatcc ctttgcgatc cagcatctgg ctggacgccg cgcgccaggt
      901 gtactgcccg tagtcataac gcagccagct accaatcagg aagaccgccc cggctatgta
      961 cgggtagata tcaaagaaga acatattcag gaattgcatt agtgctgtcc tccggtggtg
     1021 atattcagat attgcggcgc gacggcaccg gcaaagcgac gctgatgagc agtgattgct
     1081 gaatcaccgc agcctttgtc agcaaagaat ttaacctgct cttcttccca gacagcatcc
     1141 agcgcctgcg gcgtatcatc gcgcgcttcg tcggcaattt tttccgccac tttgtcgctg
     1201 tcgatagcgg tattggccaa tttcagcagc agatcaaaca gcacggcata acggctttca
     1261 cgctgttgca gacgcgcgct cagcaatgcc agaatcggcg cgatatcttt caaaccttcc
     1321 acggcttcgc tttgcggcag ctgagccagg tactccagat acagcggcag atggtccggc
     1381 aattcgcggc tgtttaactg caagccgtgc tgctcgtact gcgccaacag gtcaaccatc
     1441 gcctggccgc ggtcgcggga ttcgccgtgc acatgttcaa acagcaacag cgacgtggcg
     1501 cgaccacggt cgaacagttc gctgtactgc gcctgggcat ccagcggatc cagcgccgtt
     1561 aaatcgcgca ggaaaatgcc cagcgcatgg gcatcttctt tcgacaaatt ttccgacgcg
     1621 gcaatcgcct caaacatctc ctgttgatgc tgccataagg cagcatccgg atattcaagg
     1681 agacgcgata caatcacgag ttcgatcatg gatgcggctc cgttttgctg gtcacatcga
     1741 tggcatcgat acgacggctg ttgaacagat tgaatttggt atctgagccg tggcagccat
     1801 caccaaaggt aaagccgcag ccatttttct ccgggaaggc ttcccgtgcc agttcacgat
     1861 gactactcgg caccacaaag cgatcttcgt agttagcaat cgccagataa cggtacatct
     1921 cctgtgcctg ggcttcagtc aggccgacct cttccagcgc acgggtatca actttaccgt
     1981 caacggtttc agcacgtttg taatgacgca tcgccagcat acgtttcagt gcgcgcagta
     2041 ccggtttggt atcaccggcg gtcagcagat tcgccagata ctgtaccgga atacgcaagc
     2101 tttcgacgtc tggcagaatt ccgttgctgc ccagctcgcc cgcgtctgct gcagactgaa
     2161 tcggagacag aggcggcacg taccagacca tcggcagtgt gcgatattct ggatgcagcg
     2221 gcagcgccag tttccattcc atcgccattt tataaaccgg cgactgctgt gcagcttcaa
     2281 taacgctcag cggaataccg tctttaatcg cctgctcgat gactttcgga tcgttcggat
     2341 cgaggaacac gtccagctga cgctggtaga gatctttctc gttctcggtg ctggctgcac
     2401 gttcaatcgc gtcggcatcg tacagcagca cgccaagata acggatacga ccgacacagg
     2461 tttctgagca cacggtcggc tgacccgctt caatacgcgg atagcagaag atgcacttct
     2521 cagacttacc gctcttccag ttgaagtaga tttttttgta cgggcatccg gtgatgcaca
     2581 tacgccagcc acggcattta tcctggtcga tcaggacgat gccatcttct tcacgcttgt
     2641 aaatcgcacc gctcgggcag gtcgccacac atgccgggtt caggcagtgt tcgcacaggc
     2701 gcggcaaata catcataaag gtgttttcga actggctata catcgccttc tggatgttgt
     2761 cgaagttctt gtctttcgcc agtttgtcaa actcaccgcc caggtcatct tcccagttcg
     2821 gccctttttc gattttcgcc atccgttccc cggtaatcag cgaacgcgga cgggcaatcg
     2881 gctgcgcttt gctgccttcc ggcgcggtat gcaggttctg atagtcaaaa tcgaacggct
     2941 cgtaataatc gtcgatcccc ggcagatgcg ggttagcgaa gattttaccc agcagcatgg
     3001 cgcggttacc catgcgcggc tgcagtttgc cattgatttt acggatccag ccgcctttgt
     3061 atttttcctg gttttcccag tcagtcggga agccctggcc cggcttggtt tccacgttgt
     3121 tgaaccacgc gtattccacg ccttcacggc tggtccagac gtttttacag gtaactgaac
     3181 aggtgtggca gccgatgcac ttatcgagat tcagcaccat gccgacttgt gaacgaattt
     3241 tcattttacg ctctcctgta cctggtcatt gccttcgcca tctaaccagt caatgttctt
     3301 catcttacgc actacaacaa actcatcgcg gttagaacct acggtgccat agtagttaaa
     3361 gccgtatgcc agatgggcat agccgccgat catatgcgtc ggtttcggcg tgatacgggt
     3421 gaccgagtta tggataccac cacgctgttg ggtaatttcc gaaccaggca ggttaacgat
     3481 acgttcctgc gcgtggtaca tcatggtcat ccctgccgga acacgctggc tgacaaccgc
     3541 acgggcagtc agagcaccgt tgctgttgaa tacttcaatc cagtcgttat cggcgatacc
     3601 cagatctttg gcatcggctt cactcaacca gaccaccgga ccaccgcgac ctaaagtcag
     3661 catcagcagg ttgtcgctgt aggtggagtg gataccccac ttctggtgcg gcgtcaggaa
     3721 gttgagcgct ttttccgggt tgccattgga tttctggccc atcacttctt tcaccgaacg
     3781 ggtgtcgatc ggcggacgat aaaccagcag gctttcaccg aaatcacgca tccactggtg
     3841 atcctgatac agttgctgac gaccagagag cgtacgccac gggatcagct cgtgaacgtt
     3901 ggtgtaaccg gcgttgtaag aaacgtgttc atcttccaga ccagaccagg tcgggctgga
     3961 gatgattttg cgcggctgtg cctgaatatc gcggaagcga atcttctcgt cttctttatt
     4021 cagcgccaga tgcgtatggt cacgaccggt aaactcgctc agggcagccc aggctttcac
     4081 ggctacctga ccgttggttt ccggtgccag cgtcaggatc atctctgccg catcaattgc
     4141 ggtgttcagc atcggctggc cttttgccgg accttccgct ttggtgtagt tgagcttacg
     4201 cagcagatcc atctcgctct gggtgttcca ggcaataccc ttaccaccgt taccgatttt
     4261 ctccatcagc gggccgatag aggtaaagcg ttcgtaagtt gccggataat cgcgctctac
     4321 gaccataatg tgtggcgcgg ttttacctgg gatcaggtcg cattcgcctt ttttccagtc
     4381 tttcacatcc agcggctgcg ccagttcagc ggcagagtcg tgctggatag gcagcgtgac
     4441 gatgtcggtt tctttaccca gatggccgac gcacacttcg gagaatttct tcgcgatggc
     4501 tttgtagatt tcccagtcgc ttttcgcttc ccaggccgga tcgaccgccg cagacagcgg
     4561 gtgaataaac ggatgcatat ccgaagtatt catgtcgtct ttttcgtacc aggtcgccgt
     4621 cggcaaaatg atgtcggaat agagacaggt gctcgacaga cggaagtcca gcgtaaccac
     4681 cagatccagc ttgccttcca gaccattgtc ctgccagtcc acttcttccg gcttcacgcc
     4741 gccctgctgc cccagatctt taccctggat accgtgctcc gtccccagca ggtacttgag
     4801 cataaactca tgacctttac cggaagaacc gagcaggtta gaacgccaga tgaacaggtt
     4861 gcgcgggtgg tttttaccgt tttctggttg ttctgccgca aaacggatgg aaccctcttt
     4921 cagggatttc acggtgtaat ctaccggatt catcccggct ttttccgctt cgccagcgat
     4981 agtcagcggg ttagtgccta actgcggtgc agacggcagc cagcccatgc gttctgcacg
     5041 gacgttaaag tcgattaggt gtccggtata acgggattta tccgccatcg gcgacagcag
     5101 ttcttccgca gtcacggttt cataacgcca ctggctggag tggttataga aataagaagt
     5161 gctgttcatg tgacgcgccg gacgctgcca gtcaagggca aacgccagcg gctgccagcc
     5221 ggtttgcgga cgcagttttt cctgacctac atagtgcgcc cagccgcccc cgctctgacc
     5281 gacacagccg cagaaaatca gcatgttgat cagaccacga tagttcatat cgaggtgata
     5341 ccagtggttc agccccgcac cgacgataat catcgaacga ccgtgcgttt tatcagcgtt
     5401 atcggcaaat tcacgggcga tgcgaataat ctggctgcga gaaacgccgg taatctgctc
     5461 ggcccaggcc ggggtataag ctttcacatc gtcatagctg gttgcacagt taacgtcgtt
     5521 caggccacgt tccagaccgt agtttgccag cgtcagatca taaacggtgg tcaccagggc
     5581 ggtgctgcca tcagccagtt gcaggcgttt caccggcagt ttgtgcagca gcacgttttc
     5641 cagttccact ttgttgaagt gttcagtacc gtcgccacca aagtacggga agcccacctc
     5701 agcgatctca tcctggctac ccagcaggct cagttgcagc tcggtttctt cgccagtttt
     5761 gccgtcgcgc tgctcaagat tccatttgcc cttctcgccc cagcggaagc caatagaacc
     5821 gttcggcgca accatttcac cattggtatt aaaggcgaca gttttccatt ccggattgtt
     5881 ttcctgaccc agcgcatcca ccagatcagc agcgcgcagc atacgacccg cggcgtagta
     5941 accgtcgcgc tcttccagca tcaccagcat cggcatgtcg gtgtagcgac gcacatagtc
     6001 ggtgaaatac tggcttgggt tgtcgaggtg gaattcacgc agcattacgt ggcccattgc
     6061 cagcgccatt gccgcatcgg tgccctgttt cggtgccagc cacagatcgc acagtttggc
     6121 gatttcagcg tagtccggcg tgacggcaac agttttggtc cctttgtaac gcacttcagt
     6181 aaagaagtga gcatccgggg tacgcgtctg cggcacgttt gacccccagg cgatgatgta
     6241 gctggagttg taccagtcag cagattccgg tacgtctgtt tgctcgcccc aggtttgcgg
     6301 agacgcagga ggcaggtcgc agtaccagtc gtagaagctt aagcaagtac cgccaatcag
     6361 cgagagatag cgtgcacccg atgcgtaaga aaccatcgac attgccggaa ttggcgagaa
     6421 gccagcgaca cggtccgggc cgtagttttt gatggtgtaa acgttagatg cggcgatcag
     6481 ttcgttcact tcctgccagg aagaacgaac aaaaccaccg cgtccacgcg cctgcttaaa
     6541 gcttttcgct ttatcggcgt cttcaatgat agaagcccat gcctcaaccg gatcgctatg
     6601 cagcgccttc gcttcacgcc acattttcat caagcgtttg cgcatcatcg ggtatttcag
     6661 gcggttggcg ctgtaaagat accaggagta gctggcaccg cgcgggcagc cgcgaggttc
     6721 atggtttggc aggtccgggc gagtacgtgg atagtcagtc tgctgggttt cccaggtgac
     6781 cagaccgttt ttgacgtaga ttttccagct gcaggagccg gtgcagttta ccccgtgggt
     6841 agagcggacg attttgtcat gctgccaacg ctggcgatat ccatcctccc agtcacggtt
     6901 ggtattgaga agctggccat gcccatcggc aaaggtttca cccttctgtt tgaagtagcg
     6961 aaaccggtcc aggaatttac tcatcggttt tctcctgtgg gagcctgtcg gccctctgat
     7021 aaatcgacat tgctaatgtt taagggcaag gtaacgctct gaaacagcgt gggaattgat
     7081 aacgatcaag tgtgatgggg atgaaaaata aagtaaattc ctaagatacc cctttaggat
     7141 aattaacagt taaagcataa catgctgtaa attaagacgt tttacacctg tagttattta
     7201 aactattaag aattttatgc agatgggtat taaggagtat tccccatggg taacatatta
     7261 atggctctta atgaaatccg gcgaaataaa ccatacaaca ttagtatgtt gtaactaaat
     7321 gacgcataaa aaaagcgcga catcatgccg cgcaaaggat aatcaaatat tttactctat
     7381 ataattcggt ttacaggaag gcggcaagtg aatgaatccc aggagcttac taaagtaagt
     7441 gactggggtg aatgaacgca gccgcagcac atgtaacccg aagtatgacg agtaacggtt
     7501 tattttttag aatgccgacc atataccgcc caggtaatca ctacgcaggc gatatagaaa
     7561 atcaaaaata ctttcattgc gccgactggc gaacccgtta atgccagcga gctaccaaac
     7621 gctttcggga taaagaagcc accaatcgcg ccaatcgcag agatgaaacc caacgccgcc
     7681 gccgtgtcgg ttgccgcttc acgcatcgca cgttcgtcag aacccccttc tgctttcacg
     7741 cgatccattg tcagtttacg gaagatcact gaaatcatct ggaaagtgga accactaccc
     7801 agcccagctg tcaggaacag cgccaggaag accgcgaaga acgccataaa gcttccgccc
     7861 tgcccgtcag tcggtaaggt caggaacagc aggccgctga aaatcgccat cagaataaag
     7921 ttcaccagcg tgacacgagt tccgcccaga cggtcagata atgcaccacc tgcagaacgc
     7981 gccagcgcac caataaacgg cccgaagaaa gcgtattgca gaatctgaac atccgggaac
     8041 tgcgtttttg acagcatcgc aaagcccgcg gagaagccga taaaggagcc gaaggttgcc
     8101 agatacagca ggctcataat ccacagatgc ccccgtttga gtaccggcaa ctgctccttg
     8161 atggaggctt tcgaggtagc aagatcgttc atgccaaacc acgccgcaat ggtgaagatg
     8221 gcaaggaacg gcacccatat ccaggacgca ttcgccagat acagctcagt cccatccggc
     8281 tgtttgacgc cctggctacc aaatactgcg aaaatcgaca gtgataccac cagcggagca
     8341 accaactgca tgacgctgac gcccatgttg cccagaccac cattcagacc cagcgcgcca
     8401 ccctgcttct gtttcggaaa gaagaagctg atgtttgcca tactggaggc gaagttcgcg
     8461 ccagcaaagc cgcacagcag agagatgatg atgaagacgc tataaggcgt ggaggtatcc
     8521 tgcacggcaa aacccagcca gacacaagga ataatcagaa taccggtgct gaacgccgtc
     8581 cagcgacgac caccgaagat aggaaccata aaggagtatg gaacacgtaa taacgcgcca
     8641 gaaactgaag gcagcgcagt caacataaat agctgatcgg tcgtaaaatt aaaaccgact
     8701 ttcggtaagt tcaccgcaac agcgctgaac aacatccata cacaaaacgc cagcagcaga
     8761 cagggaacgg aaatccacag gttgcggctg gcaatacgtt gaccgcgttg ttgccagaac
     8821 gcaggatctt ccggtcgcca atctgtaatg acagctccag tagccctttc gggggcggat
     8881 gagtgactca tagacacctc tgatactcgt ttcgatgtct gccaccttag tgtctgtagc
     8941 taaaggcaat ttgatgtaaa tcaaacgata agataacttt atctatcatt gatatttatc
     9001 attacccata gtgagtacag tgatttcata aaaattatga gatttttcac ggtgctgtaa
     9061 aatccctacc cttaccgatg taaagcgact aaccacacgg caaataagga gtaactcctt
     9121 ccgggtatgg gtatacttca gccaatagcc gagaatactg ccattccaga atgtatcgtc
     9181 acattcatta aggttattgc tcatttaaag cctgaaggaa gaggtttaca tgctcaaacg
     9241 ttgtctctct ccgctcaccc tggttaatca ggttgcgctt attgtgttgc tttctactgc
     9301 tattggactg gcagggatgg cggtttctgg ctggctggtg caaggcgttc agggcagcgc
     9361 ccatgcgatc aacaaagcgg gatcgctgcg catgcaaagt taccgtctgt tggcagcagt
     9421 gccattaagc gagaaagaca agcccttaat taaagagatg gaacaaacgg catttagcgc
     9481 cgagttgact cgagcagcag aacgagacgg acaactggcg caattacagg gtttacaaga
     9541 ttactggcgt aatgaactga tccctgcgct gatacgtgca caaaaccgcg aaacggtgtc
     9601 agcggatgtc agccagtttg ttgccgggct tgatcagctg gtatctggtt ttgaccgcac
     9661 cacggaaatg cgcattgaga cagtggtact ggtccatcgg gtaatggcgg tatttatggc
     9721 acttttactg gtgttcacta ttatttggtt gcgggcgcga ctgctacaac cgtggcggca
     9781 actgctggca atggcgagtg ccgtcagcca tcgcgatttt acccaacgcg cgaacatcag
     9841 cgggcgcaac gaaatggcga tgcttggaac tgcattgaac aatatgtctg cagaactggc
     9901 cgaaagttat gccgtacttg agcagcgggt tcaggagaaa accgccgggc tggagcataa
     9961 aaatcagatc ctctcttttt tatggcaggc taaccgccgt ttgcattccc gcgccccgct
    10021 gtgtgaacgc ctgtcacctg tactcaacgg cttacagaat ttaaccctgc tacgtgatat
    10081 cgaactgcgg gtttatgata ctgacgatga agagaatcat caggagttta cctgccagcc
    10141 agatatgact tgtgatgata aaggctgcca gctctgcccg cgcggcgtat tacccgttgg
    10201 cgatcgcggc acgaccctga agtggcggct ggctgactct catacgcagt acggtatttt
    10261 gctggcgacc ctgccgcagg ggcgtcatct tagccatgat caacaacaac tggtggatac
    10321 cctggttgaa caactcaccg ccacgctggc gctggatcgc catcaggaac gtcagcaaca
    10381 gttgatcgtg atggaagagc gtgccaccat tgcgcgcgaa ctgcatgatt ctattgccca
    10441 atctctctct tgcatgaaga tgcaggtgag ttgtttacag atgcagggcg atgcgctgcc
    10501 agaaagcagc cgcgaactgt taagtcagat ccgtaacgaa ctgaatgcat cctgggcgca
    10561 gttgcgtgaa ttgctcacca cattccgctt gcagctcacc gagcctggat tacgtccggc
    10621 gctggaggcg agttgcgaag agtacagcgc caaatttggc ttcccggtga agctggatta
    10681 tcaattgccg cctcgtctgg tgccttcgca tcaggcaatc cacttgttgc aaattgcccg
    10741 tgaggcatta agtaacgccc tcaaacattc gcaagcgagt gaggtcgtgg tgacggtggc
    10801 gcaaaacgat aatcaggtaa aactgaccgt tcaggataat ggctgcggcg tgcctgaaaa
    10861 tgccatccgc agcaatcact acggcatgat aataatgcgc gaccgtgcgc aaagtttacg
    10921 aggcgattgc cgcgtccgcc gtcgtgaatc aggtggcacc gaagtggtgg tcacctttat
    10981 tcccgaaaaa actttcacag acgtccaagg agatacccat gagtaatcag gaaccggcta
    11041 ctatcctgct gattgacgat cacccgatgc tgcgaactgg cgtaaaacag cttatcagta
    11101 tggcaccaga tatcaccgtg gttggcgaag cgagtaatgg cgaacagggt attgaactgg
    11161 cggagtctct tgatcccgat ctgatcctgt tagatctcaa tatgcccggc atgaacggtc
    11221 tggaaacgct ggataaactg cgcgaaaagt ccctctcagg gcgcattgtg gtattcagcg
    11281 tctctaacca tgaagaagat gtggtcaccg cactgaaacg cggcgcggat ggctatctgt
    11341 taaaagatat ggaaccggaa gatctgctga aagcattgca tcaggcagct gctggcgaaa
    11401 tggtattaag cgaagcatta acgcctgttc tggccgccag cttgcgcgct aaccgtgcca
    11461 ctactgagcg cgatgttaac cagttaaccc cacgcgagcg cgatattctc aagctgattg
    11521 cccagggttt gccgaacaag atgattgccc gccgcctgga tatcaccgaa agcacagtaa
    11581 aagtgcacgt caagcacatg ctgaagaaaa tgaagctcaa gtctcgcgtg gaagcagcgg
    11641 tatgggtgca tcaggagcgc attttctgat tacggttccc agcgcagttc gtcgtttgac
    11701 aatgcatcga acggttcgac aagcgttagc gtgatctcat tggaggagac acgctgcccc
    11761 tggttatctt ccaccaccac cgataatcgc cagtgattgc ttgccccttc cccgttctgc
    11821 cagtcaggca tgatcagcgt ccagccctcc gcgctgttgg cttgtgcgcc cggcgtcaaa
    11881 cttaatatct gcgtatcgcc ctgccaaatc agttgccgaa taccgtaacg actgcggatt
    11941 tgtaatttca gcggcactgt ttcgccaggt tttagatccc acggcggtgt cgccagaaac
    12001 accgttaacg tttttcgctg tcggtactca agagtcggta gattatttcg ctgcggattg
    12061 tcatagcgac taccacgtaa cgactgactt tcggcaacct cgcccgcaga aagttgtttt
    12121 ttgagcggta caccaaagcg gtaattaaga ttcagcccga ggttattttg attctcgcca
    12181 ctttcaccct gtttatgctg ggccgtcaca gtgactaatg gcacaggggt gtaatttaat
    12241 cccagactca acgcgacggg attgtgataa cccgtaccag agttaaacaa atcaacacga
    12301 tcaccaaaat actgttctac gctgacactg gtattgaggt gttgatagaa cggcatgcgc
    12361 atccgggctg tcaggtcata cccgcgcgcc atccgttgtt cctgcgtggc tgtctgttca
    12421 tgccatgcag caaacggctg ataaaagttt gccgatagtc gcaaatattc gccccacgct
    12481 tcggcaccaa agcccgctcg ctgaagattt tcgtccagca agttgtcata aaaagtgtta
    12541 taacccacca gccagttgcc gcgcgcccag cgttgcccaa cgcccacatt gctcaccaac
    12601 ccatcatcct gctgagtaag accaagctgg ctccaggtga gataacgatc attatcttgt
    12661 aacggcacaa accagcttcc acgactgccg gtgaaatgtc cttcgttatc cactttgacg
    12721 tcaacactgg catttcccca cggtgatagc caggactcta cgtgctgatt aacctgttga
    12781 ctaagcgcgt cgcggacttt tcccaatgcg aaagcttttg cctgctcgcc agtatccagc
    12841 ccgttatcat tcatactggt ttcgccgaaa tctttcacaa tttcagcaaa gtgtttttcc
    12901 ccatcatgat tttcgggagc catgcctaaa tccggcagac catcattgtt attatcaaag
    12961 ggatttgccg ctttttgctc gaaggtagat tgtgcgttag ctgtaccgcc tgctacaagc
    13021 aagagtaaat aaaacgggat aatgcgagga acgaaacggc tcaaaacgtc ggtaacttcc
    13081 ggtaataatg aaaaaccata gccttaaaga ataacgatta ctggcaactt ttgtcgtatt
    13141 ttctcgcgct ttcaggaaaa tccttaaaaa tgtgaccgat tggtcagacg ctgcagaagc
    13201 agtaagatag aagatgaatg tctaattccc tgaatacagg agtaaataat gcaaaaaatc
    13261 gtgatcgttg ccaatggcgc accttacggg agcgaatcct tgtttaacag cttgcggctg
    13321 gccattgcgt tacgagagca ggagagcaat ctggatctgc gtctgttcct gatgtctgat
    13381 gcggtcacag ccgggttgcg cgggcaaaaa ccaggggaag gctacaacat tcagcaaatg
    13441 ctggagatcc ttaccgctca gaatgtaccg gtgaaattgt gcaaaacctg taccgacggg
    13501 cgcgggatta gcacacatcc tcttattgat ggggtggaaa tcggtactct tgtggaactg
    13561 gcgcaatgga cgctgtcagc cgataaagtg ctcacatttt aatgtttgag ggggcgtctg
    13621 acgccccact atgagggaag tttactcagg cgaatcccgg ccgtaacaca ccatccggat
    13681 aattctccgc cagcagtttt tttaccgata ccagcaaatc attcagccca tcgtcctgca
    13741 ttcccagttt gataagctcc tgttccagtg aaaacaggta ttgtgcgttg gttcccagcg
    13801 gaccgctcgc cgccgcaatc aacggcgcga tgacctgagc gcgagtgtca gattcatatt
    13861 ctggatgtcg cggctccata ataaacacaa tggcgtttac tgtgcatcca tcatcaagat
    13921 caagctgaca ccaggttggc agataacagc cggtaatcat ctcgcgcttc cacaacaggg
    13981 ttagttcctg ctccagcgtc tcttctggca gtcgataggc gacgcctgta gtgcgtccgc
    14041 cctctttcag tgcaagcatc cgtcccggct ggtgcgcagt cccgcgcccg gcggtcaggc
    14101 gcaggcaaaa cgcgcgatgc catccaacca gtgtaccggt gcacgactcg gtaaactcca
    14161 gtgccggatt ccacatcaac gatccataac cgaagatcca caccggtccc tcatcaggtc
    14221 gacaagccag cgtcgccgcc agcgaagccg cccgttgctc tgctgaccat aagagtgatt
    14281 cttcaatggc accaaatgcc gttttacaat cggcattcat caagaaatca cgcgttatca
    14341 ccttacacct ccgccactgc atacttccct gcgacaactt tttggtgtct tccggacatt
    14401 tttactgctc attttgaaac cactaacggg acaatatgca atccacggac cacttgcaag
    14461 gcaacaccgc acgaatagct ttaactaagc taccggtttt acgatttttt atgccattta
    14521 tcatcatccc ctttggcata ttcatgcttc acagcagccc aggccacttt atgcgcggtt
    14581 tcttcgcgac tggcgtcatc acgccgatcg tctttatctt tatattgatc ccatgcgctg
    14641 ttgaacgctt ctttatagat atcctgggca tgagacggta gaacgtgctt tacgctttcc
    14701 ggcagatcgc ttttcgtttt atacggcatc gttgactcct tttacaaagt tgaacattaa
    14761 gtgtggtcaa caatatctac tcttgccaaa acataagaat tttcatgtca gactgttctt
    14821 aagaaacgct aaaacaaaca ttgtctaaac gtaatactta tttttatgtc ccaatgagta
    14881 aaaaatcgac agtggggtaa aaattcgtaa aatgttaccg atatccagac actttctgtt
    14941 atttttccta cctatttttt acattttcga taaattcatc tgcaaaggga taaccataat
    15001 gtcaaatgct caagaggcgg taaaaacccg ccacaaggag acttcgctta ttttcccggt
    15061 tctggcgctg gtagtgctgt tcctgtgggg aagcagccag acactaccag tggtcattgc
    15121 catcaatctt cttgcgctta ttggtatttt aagtagcgcc tttagtgttg tccgtcatgc
    15181 ggacgtatta gcccatcgcc tgggagaacc ttacggttcg cttattctta gcctttcagt
    15241 ggttattctt gaagtcagtt tgatttcagc tttaatggca accggcgacg ccgcgccaac
    15301 gctaatgcgt gatacgctct attcaatcat tatgattgtt accggtgggc tggttggctt
    15361 ttcattattg ttgggcggtc gtaagtttgc cacccaatat atgaatctgt ttggtatcaa
    15421 gcagtattta attgcgctgt tccccctggc gataatcgta ctggtatttc caatggctct
    15481 gcctgcggcg aatttttcaa ccggtcaggc gttactggta gcattaattt ctgcggcaat
    15541 gtatggcgta tttttgctga tccagaccaa aacgcatcaa agtttgtttg tctacgagca
    15601 cgaagatgac agtgatgacg atgacccgca tcacggtaaa ccgtctgccc atagcagcgt
    15661 gtggcatgct atctggttga ttatccatct gattgccgtt attgcagtca ccaaaatgaa
    15721 tgccagcccg ctggagacat tgctcgacag catgaatgcc cctgtcgcct ttactggctt
    15781 cctggtggct ctgttgattc tgtcgccgga aggtttaggt gcgttaaaag cagtgttgaa
    15841 caaccaggtc cagcgcgcga tgaatctgtt ctttggttca gtgttagcaa ccatttcgct
    15901 taccgtacct gtcgtcacgc taattgcctt tatgacgggt aacgaattac agtttgcact
    15961 tggtgcgcca gaaatggtgg tgatggtggc ctctttagtg ctgtgccata tctccttctc
    16021 caccggacgt actaacgtgc tcaatggcgc agcgcatctg gcactgtttg ccgcctattt
    16081 gatgacgata tttgcctgac agttatatct cctccgatga cggaaatcgg gggagataag
    16141 ataaattcct gcttattgta tttcttcata ctacctggac agctcgttaa tcagaattgc
    16201 gagcctgtac cacaacctgc acgaaaccac tgcaacagct gttgaaaagc cggaatgtac
    16261 tctttattgc aaacatttca gggatgaagt gaagatgaat tgagacgtag tatccacacc
    16321 aagccggaat tatccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt
    16381 tgctggaaag agaaaacccc cgcacgttga gaggtaaaac ctgacaacag aacgggggct
    16441 aatcttgaaa ccagacactt caagaatagc cgcaaaatgt tgtcattaca acaaggcggc
    16501 tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc accgatcctg
    16561 gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta aggtgaaaac
    16621 gagcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg gtaacagcct
    16681 gcggcctttt ttgtatctgg cacccatgaa aaaagtctta acgcagaacg ctaagacttt
    16741 atttttcaag caaaaaagat tacttgctgg tatccagttc ttcgaaacct ttcaccagat
    16801 catcaatcgc tttcatctgc ttaaggaacg gttccagttt agccagcggc agcgcggatg
    16861 gaccatcaca tttcgcatgt tccggatccg gatgcgcttc aataaacagc cccgccagac
    16921 ctaccgccat accggctcgt gccagctcag ccacctgagc acgacgacca ccggaagctg
    16981 cgccaaacgg atcgcggcat tgcagtgcgt gggtcacgtc gaaaatcacc ggcgagttac
    17041 cagacacttt cttcataatg ctgaagccca gcatatcgac aaccaggttg tcatagccga
    17101 agttagcacc acgatcgcaa agaatcactt tttcgttgcc gccttctttg aatttatcaa
    17161 cgatattacc catctgtccc gggctgacaa actgtggttt cttgacgtta attaccgcac
    17221 cggttttcgc catggcttca accaggtcag tctggcgagc aagaaacgcc ggcaactgaa
    17281 tcacatccac gacatcagca acgggctgtg cctgacttgg ttcgtgaacg tcggtgataa
    17341 ttttcacgcc aaaagtctgc ttcaactcct ggaagatttt catcccttct tccaggcccg
    17401 gtccacgata agagtggatg gaggagcggt tggctttgtc aaaagaggct ttgaacacgt
    17461 aagggatacc cagtttctgg gtcacagtta cgtagtgctc gcaaatgcgc atcgccagat
    17521 cgcgagattc cagcacgttc ataccgccaa acagtacgaa cggcaggtca tttgctacgt
    17581 tgatgtcgcc aatgctaacc actttttgtt tcataggatc gccttaatct tgagtaaaat
    17641 gtcgattaat taatgcagca caatatgttt atgcgcgatg ttatttattt gcgcacggat
    17701 catttcgctg atcgggtctt ccggacactg ttcaacgaaa taacttaaat cgttcaacgc
    17761 aacgtgttcg caatccagtt gcgcataaat caacccgcga tcgcgaattt catagggatc
    17821 ttcagggttg aattgtaata aagcttcgct ggtgcgtaac gccagctcca tctgattttc
    17881 ttccatcaac gaggctttga gtgtatccag caatttgcgg attacctcaa tgttatcagc
    17941 ttcatcaagg tcttcataaa acagttccgc cgacgggctg atatttccct ttaaccatac
    18001 gtccagcata tgttcgctta acgattcacc gttaaaagga ttaatcagcc aaatttcgcc
    18061 atccggacat tcaatgcgca atatcagctg cgtagggaaa atcaccggca gcagcggcaa
    18121 atcgagacga ttcgcgaccc ataataaaac cgcacctaat gataccgcac tgccctgtcg
    18181 attctttaac acctggtcca gccataatgc atcggaaaga cgataaacgc cgcgtgaggc
    18241 tttaaatccc cagtcgccgt aaaacagcgc gatcagtttt tccagctgct cttctaaagg
    18301 cagaagctgg ctgatttctt ccttcgccag actaacgaga cgctccagtt cgtcgtaaac
    18361 atcttgcgag ggaaaatcgc ggcggattgc ttcgcaagcc aggatcatgc cttcgcacaa
    18421 tggcgcttta ttaaattcga aatcagctaa cgatctcatg acttacccca gtaacggtac
    18481 ttttgtggtg gcgagtttaa tgatgatgta cagcaccacc aacgccagcg ggaaggcaat
    18541 aatgcgcgcc tgctgactgt gcatacgacg ataatcgagt gcaataaaac ccaaaacgat
    18601 ataaatgata actccaaaca gcttttcagt cagccactgt gcctgcccgg aaaatggcag
    18661 gatgtgcgct ttagccatca acgcaatgcc gcttaacaat aacaccgtat cgacaaccgg
    18721 cggcacaatg cgcgtccagc gagcaaatgc ctgtggatgc ttctgataac gtagccagaa
    18781 acgtaaggtt aatagcccaa cagaaagcgc gagactaata agatgaacac taagcagtgt
    18841 agaaaaactt gtcattgata atagcggccg agcgttacgc gctcgttatc accatagtca
    18901 cggcaggttt cgacgtcatg ataccccgcg aggataaatg cttgtcgcac cgcttcgccc
    18961 tgctgccagc catgttccag aagcagaaag ccgccggata ccagcgcgtt acgcgactgt
    19021 tcgatgatat gcacgatgtc tgccattcca ctgtctgccg caaccagcgc agtgagcggc
    19081 tcaaagcgga catcgccttg ttgaagatgt gggtcctgct cgtcaatata cggcggattg
    19141 ctgacaatca tcgcaaactg ctgcccggct agcgcgctaa accagtcgct ttgcagaatg
    19201 tggatatttt tgatcgccag atgctgggca ttacgttgtg ccagggagac agcatcaggc
    19261 atacgatcta cagcgataat ttcgcagtcc gggcgctcgc tagccagcgc aagcgcaatc
    19321 gccccggtac ccgtcccgag atcaagaata cggcaaggtt gttcaggcaa ccgcgccagt
    19381 gcctgctcca ccagacactc cgtatccggg cgcggaatta aggtcgctgg cgaaacaaat
    19441 aacggcaacg accagaattc tcgcaccccg gttaaatgag caatgggttc accatcgcga
    19501 cgacgtgtca gtagcgcatc aagttgctga cattgttcgt cagtcagatg cgtttcacca
    19561 aaggcgagaa taaaagtacg ccctctgccg gtaacatgct ccagcaggat ttcagcatca
    19621 cgccgcgggc tttcgctcgc ctgaagttgg cttattgctt cacgtaacca gtgttgatat
    19681 tccattattc ctgctcggac aacgccgcca gttggtcggc ctgatgttcc tggataatcg
    19741 gttcaatcag catatccagc ttaccttcca tcacttcatc caggcggtag agcgtcaggt
    19801 tgatgcggtg atcggtaacg cgcccctgcg ggaaattgta agtacggtta cggtcgctgc
    19861 gatcgccact ccccagcagg ttacgacggg tagacgcttc ggcctgttgg cgttttgcca
    19921 tttcagcagc gtggatgcga gcaccgagca ctgaaagcgc cttcgctttg tttttatgtt
    19981 gtgaacgttc atcctgacat tcaacaacaa tcccggtcgg caagtgagta atacgaattg
    20041 ccgaatcggt ggtgttaacg tgctgaccac ccgcccctga cgagcggaaa gtatcaatgc
    20101 gtaaatctgc tgggttgatg tccggcagtt ctgcgtcagg cagttctggc attaccgcaa
    20161 cggtacaagc agaagtatga atacgaccct gcgattccgt agcaggaaca cgctgcacgc
    20221 gatgaccgcc agattcaaat ttcagacgac catacacacc atcaccgcta attttggcga
    20281 tgatctcttt ataaccacca tgttcaccct cgctggcgct catgatttct acccgccagc
    20341 ggcgggcttc ggcgtaacgg ctgtacatac ggaacagatc gcctgcgaac agcgccgctt
    20401 cgtcgccgcc ggttccggct cggacttcga ggaaggcgtt acgttcgtca tcaggatctt
    20461 ttggcagtaa cagaacctgt aattgctgtt ccagttgctc gcttttttct ttagcttcgc
    20521 gcagttcatc ctgcgccatc tcacgcattt caggatcatc gagcatcatc tgtgcggttt
    20581 cgatatcttc ctgaacctgt tgccagtcgg taaaacagcg cgaaacatca cttaactgcg
    20641 catattcgcg tgataatgcg cgaaaacgtt cctggtcggc gatagtttgc gcgtcaccca
    20701 gcaacgcctg aacttcttca tggcgttcat gcagggcttc cagtttggca acgatagaag
    20761 gcttcatagg cgtaaatgca ccctgtaaaa aaagaaaatg atgtactgct actccagccc
    20821 gaggctgtcg cgcagaatat tcaggcgttc gttatccccg tcacgggcgg cctgttgaag
    20881 tgatttcgtt ggcgcatgga tcaagcggtt agtcagtttc catgccagat cctgcataat
    20941 ggcttgcgcg tcgccgccct gctcaagggc cgctaacgct ttggcggtta actcatcgcg
    21001 aacgtgctct gcctggctgc gatactcgcg aatggtttcg ctggcgcttt gtgctcgcag
    21061 ccacgccata aattcgctgg tttcctgagc gacaatagtt tccgcctcaa ccgctgcggc
    21121 tttacgctgc gccaggttgt gcgaaatgat gctttgcaga tcgtccacac tataaagata
    21181 agcattcgcc agtttgccaa cttccggctc aacatcgcgc ggaacggcaa tatccaccaa
    21241 cagcattggt tggttgcgac ggctttttaa tgcacgctcc accatgcctt tcccgataat
    21301 cggtaacggg ctggcggtgg aactgatgat gatatcggct tcgcgcagac gttcgtcgat
    21361 ctcactcagg gcaatcactt ccgcgccaac ttcatctgcc agaatttggg cacgttcgcg
    21421 agtgcggttg gcgataatca tcttctgtac tttatgttcg cgcagatgac gcgctaccag
    21481 ctcgatggtt tcgcccgcgc ctaccagcaa cactgtgacc gtagagagcg attcaaagat
    21541 ctgccgcgcc agcgtacaag ccgcaaaagc gacagacaca gcactggcac cgatatctgt
    21601 ttcagtgcga acgcgtttcg cgacagagaa agatttctgg aacatgcgtt ccagttcgct
    21661 ggccttcata tgaccttttt gcgaatcggc aaacgctttt ttaacctgac cgaggatctg
    21721 cggctcccca agaaccagtg aatccaggcc gctggcaaca cgcattaaat ggctaaccgc
    21781 gtcgttatcc tgatgccagt agaggctttt acgcagatct tcttcattaa gattgtgata
    21841 atcgcaaagc cagcggatta acgcctcttg caggttatcc tgctcttcaa cgctaagata
    21901 aagttccgtg cggttgcacg tcgacagcac cacgccgccc tgcaccatcg gctgcgcaag
    21961 caggctgtca agcgcctgat cgagcttatc cggcgaaaac gatacacgtt ctcgcagcga
    22021 tacaggtgcc gttttatggt tgataccgag tgctaaaagg gtcatgtctg cgggaaataa
    22081 taccaacgtt gatagggtta gtctgcttgc atcatacagg atgcgtaaga tcaataaaag
    22141 agagcgcccc cttttggagt aattgccgaa agccgttaga ttctggcaat taagacaact
    22201 tgaacataga cgatagcgga cggtaacgct agcattaagg gttataactg caacgtatct
    22261 caaggacttg tcatcactat gcccctgccc gattttcgtc ttatccgcct gctaccgctg
    22321 gctgctcttg tgctcactgc ctgttccgtt accacgccca aaggtcctgg caaaagcccg
    22381 gattcgccac aatggcgtca gcatcagcaa gacgtgcgca atcttaatca gtatcagact
    22441 cgcggcgcgt tcgcttatat ttctgaccaa caaaaagtgt acgcccgctt tttctggcag
    22501 caaaccggcc aggatcgcta ccgtctgctg ctcactaacc cattgggcag cacggaactg
    22561 gagctgaatg ctcaaccggg taacgtgcag ttagtcgaca ataaaggtca gcgttatacc
    22621 gccgatgacg ccgaagagat gattggcaaa ttgaccggaa tgccaattcc gctcaacagc
    22681 ttgcgccagt ggattttagg tttaccgggt gatgcaaccg actacaaact ggacgaccag
    22741 taccgcctga gcgaaattac ctacagccag aatggcaaaa actggaaggt tgtttatggt
    22801 ggttatgaca ccaaaacgca acctgcgatg ccagccaata tggaactcac cgacggtggt
    22861 caacgcatca agttaaaaat ggataactgg atagtgaaat aatgcggaca cagtggccct
    22921 ctccggcaaa acttaatctg tttttataca ttaccggtca gcgtgcggat ggttaccaca
    22981 cgctgcaaac gctgtttcag tttcttgatt acggcgacac catcagcatt gagcttcgtg
    23041 acgatgggga tattcgtctg ttaacgcccg ttgaaggcgt ggaacatgaa gataacctga
    23101 tcgttcgcgc agcgcgattg ttgataaaaa ctgcggcaga cagcgggcgt cttccgacgg
    23161 gaagcggtgc gaatatcagc attgacaagc gtttgccgat gggcggcggt ctcggcggtg
    23221 gttcatccaa tgccgcgacg gtcctggtgg cattaaatca tctctggcaa tgcgggctaa
    23281 gcatggatga gctggcggaa atggggctga cgctgggcgc agatgttcct gtctttgttc
    23341 gggggcatgc cgcgtttgcc gaaggcgttg gtgaaatact aacgccggtg gatccgccag
    23401 agaagtggta tctggtggcg caccctggtg taagtattcc gactccggtg atttttaaag
    23461 atcctgaact cccgcgcaat acgccaaaaa ggtcaataga aacgttgcta aaatgtgaat
    23521 tcagcaatga ttgcgaggtt atcgcaagaa aacgttttcg cgaggttgat gcggtgcttt
    23581 cctggctgtt agaatacgcc ccgtcgcgcc tgactgggac aggggcctgt gtctttgctg
    23641 aatttgatac agagtctgaa gcccgccagg tgctagagca agccccggaa tggctcaatg
    23701 gctttgtggc gaaaggcgtt aatctttccc cattgcacag agccatgctt taagccgggc
    23761 aagctgagtt tcggtgacaa cgtcaccttg ttccagacgt tgcatcgcgc tctttaatac
    23821 accgcctgga aaggatcatg cctggcccgc acagttttcg gcagattctt tccaccaatg
    23881 gacgcatgcc tgaggttctt ctcgtgcctg atatgaagct ttttgctggt aacgccaccc
    23941 cggaactagc acaacgtatt gccaaccgcc tgtacacttc actcggcgac gccgctgtag
    24001 gtcgctttag cgacggcgaa gtcagtgtac aaatcaacga aaatgtacgc ggtggtgata
    24061 ttttcatcat ccagtccact tgtgccccta ctaacgacaa cctgatggaa ttagtcgtta
    24121 tggttgatgc cctgcgtcgt gcttccgcag gtcgtatcac cgctgttatc ccctactttg
    24181 gctatgcgcg ccaggaccgt cgcgtccgtt ccgctcgtgt accaatcact gcgaaagtgg
    24241 ttgcagactt cctctccagc gtcggtgttg accgtgtgct gacagtggat ctgcacgctg
    24301 aacagattca gggtttcttc gacgttccgg ttgataacgt atttggtagc ccgatcctgc
    24361 tggaagacat gctgcagctg aatctggata acccaattgt ggtttctccg gacatcggcg
    24421 gcgttgtgcg tgcccgcgct atcgctaagc tgctgaacga taccgatatg gcaatcatcg
    24481 acaaacgtcg tccgcgtgcg aacgtttccc aggtgatgca tatcatcggt gacgttgcag
    24541 gtcgtgactg cgtactggtc gatgatatga tcgacactgg cggtacgctg tgtaaagctg
    24601 ctgaagctct gaaagaacgt ggtgctaaac gtgtatttgc gtacgcgact cacccgatct
    24661 tctctggcaa cgcggcgaat aacctgcgta actctgtaat tgatgaagtc gttgtctgcg
    24721 ataccattcc gctgagcgat gaaatcaaat cactgccgaa cgtgcgtact ctgaccctgt
    24781 caggtatgct ggccgaagcg attcgtcgta tcagcaacga agaatcgatc tctgccatgt
    24841 tcgaacacta atcgaacccg gctcaaagac ccgctgcggc gggttttttt gtctgtaata
    24901 tccatttgta tgacctatgc ctccttcacc tgccatttag ttgacagatg atgcgctcac
    24961 ggatgaaaca ttattgtgaa caaaatattt tcctcacatg tgatgccttt ccgcgctctg
    25021 atcgacgctt gctggaaaga aaaatatact gccgcacggt ttacccgtga cctgattgcc
    25081 gggataaccg tcgggattat tgctatcccg ctggcgatgg cgttggctat tggtagtggt
    25141 gtggcacccc agtacggttt atataccgca gctgttgcgg ggattgtcat tgctctgacg
    25201 ggtgggtcac gctttagcgt ttccggtccg actgcggcat ttgtggtaat tctctatccc
    25261 gtgtcgcaac agtttggact ggcaggactg ctggttgcga ccttgctgtc ggggattttt
    25321 ttgattctga tgggtctggc acgctttggt cgcctgattg agtatattcc ggtttccgtc
    25381 accttaggtt tcacctcggg tatcgggatc accatcggta ccatgcagat taaagatttt
    25441 ctcggtctgc aaatggccca tgtcccggaa cattatctac aaaaagtcgg cgcattattt
    25501 atggcgctgc cgaccattaa tgtgggtgat gctgccattg gcattgtgac gctaggtatt
    25561 cttgtttttt ggccgcgtct gggcattcgt ttacccggtc accttccggc cttgctggct
    25621 ggttgcgcgg tgatggggat tgttaacctg ctcggcggac atgttgctac catcggttcg
    25681 caattccact acgtcctggc cgatggttct cagggtaacg gtattccgca actgctgccg
    25741 caactggtgc tgccgtggga tctgcctaat tcagaattca cgctaacctg ggattctatt
    25801 cgcacactgc tgcctgcggc attctcaatg gcaatgctcg gcgcaatcga atctctgctc
    25861 tgcgccgtgg tgctggatgg tatgaccggg acgaaacaca aggcgaatag cgaactggtt
    25921 ggacagggac tggggaatat catcgctccg ttctttggtg gcattaccgc tacagctgcc
    25981 atcgcgcgtt ctgccgctaa cgtccgtgcc ggggcaactt cccctatctc ggcggtgatc
    26041 cactctattc tggttattct tgccctgctg gtactggcac cgctgctctc ctggctgccg
    26101 ctttccgcca tggctgccct gctgttgatg gtggcgtgga acatgagtga agcgcacaaa
    26161 gtggtcgact tgctgcgtca tgcgccgaaa gatgacatca ttgtcatgct gctgtgcatg
    26221 tcgctgaccg tgctgtttga tatggttatt gccatcagcg tggggatcgt gctggcatcg
    26281 ctgctgttta tgcgtcgtat cgcacgtatg actcgcctgg caccggtagt cgtagatgtt
    26341 ccagacgatg tcctggttct gcgcgttatt ggcccgctgt tttttgctgc tgctgaaggc
    26401 ttgttcacgg acctggagtc acgtcttgaa ggcaaacgga ttgtgattct gaagtgggat
    26461 gcggttccgg tacttgatgc tggtggtctt gatgcgttcc agcgttttgt gaagcgtctg
    26521 cccgagggat gtgaactgcg cgtgtgcaac gtggaattcc agccactgcg cactatggct
    26581 cgcgctggca ttcaaccgat cccgggacgc ctggcgttct tcccgaatcg tcgcgcggcg
    26641 atggcggatt tataagtacg agattgacca gtcagcgcaa actgactggt cagcaaactg
    26701 cattatttgt tagctatgac ggcgattatc gctacggcgg caacgtttgt catagtggcg
    26761 aacccaccag taacggtcgc acacctgttc atgcccgcca acacgggcac ccgccagcca
    26821 gagaatggca ccgacgaaaa tacttagcac tgcaccatgt gcgaaatact ggggcatatt
    26881 aaactgtggt aactggttga ggattgaata ccccacgccg accaccatta ccaccagacc
    26941 caaccccatg agcacgttac cgagtaacga agcgtttttg cgtttcatat gtcacctccg
    27001 gaactttctg ggttgtaaca gggaataccc ctcttcctta tgtgtaaagt atagacaaca
    27061 ccctgtgggt tatgtgcggg cgtgatcaca attacaaccc ttatttcaac aaaactttac
    27121 aaataaacgc ctgaacacct tgactttcta atagtccact catgcaatta ttacgttatc
    27181 tgcatttgat acgcagtttt ttgtcaagcg gggccgcaac cagtaaacta cgcgccagtt
    27241 atgtacacac tcaggacaaa aaaacgtgac gattaaattg attgtcggcc tggcgaaccc
    27301 cggtgctgaa tacgccgcaa cgcgacataa tgctggtgcc tggttcgttg acttactggc
    27361 agagcgtttg cgcgctccgc tgcgcgaaga ggctaaattc tttggttata cttcgcgagt
    27421 cactcttgga ggcgaagatg tccgcctgtt agtcccgact acatttatga atctcagcgg
    27481 caaagccgtt gcggcgatgg ccagtttttt ccgcattaat ccggacgaaa ttctggtggc
    27541 ccacgacgaa ctggatctgc ctcctggcgt cgccaaattt aaattgggcg gtggccatgg
    27601 tggtcacaat ggactgaaag acatcatcag taaattgggt aataacccta actttcaccg
    27661 tttacgcatc ggaatcggtc atccgggcga taaaaataaa gttgtcggtt ttgtgttagg
    27721 caaaccgcct gttagtgaac agaagttaat tgatgaagcc atcgacgaag cggcgcgttg
    27781 tactgaaatg tggtttacag atggcttgac caaagcaacg aaccgattgc acgcctttaa
    27841 agcgcaataa gtcgttgtct gcggcatttt tgccgagtgc cgtgtataat aggcaaagtt
    27901 atttccattt ctgcaatctg ttagcaataa caggttgatt attaagatat taaggtgatt
    27961 taaatcatgg gattcaaatg cggtatcgtc ggtttgccca acgtcgggaa atctaccctg
    28021 ttcaacgcgc tgaccaaagc cggtattgaa gcggccaact ttccattctg caccattgag
    28081 ccgaacacag gcgtcgtacc aatgcctgac cctcgcctgg atcaactggc tgaaatcgta
    28141 aaaccgcaac gtacgcttcc cacgaccatg gaatttgtcg atatcgccgg tctggtaaaa
    28201 ggcgcatcga aaggcgaagg tctgggtaac cagttcctga ccaacatccg tgaaaccgaa
    28261 gcgatcggtc acgttgttcg ctgctttgaa aatgacaaca tcattcacgt ttccggcaaa
    28321 gttaacccgg ctgacgatat tgaagttatc aacaccgaac tggcgctggc agacctcgac
    28381 acctgcgaac gtgcgattca tcgcgtacag aagaaagcca aaggtggcga taaagacgcg
    28441 aaagctgagc tggcggtcct ggaaaaatgc ctgccccagt tggaaaatgc aggtatgctg
    28501 cgcgcgctgg atttaagcgc tgaagagaaa gcggctattc gttacctgag cttcctgacg
    28561 ctgaaaccaa caatgtacat cgccaacgtc aacgaagacg gttttgaaaa caacccatat
    28621 cttgaccagg tgcgtgaaat cgcggcgaaa gaaggttctg ttgtggttcc ggtttgtgct
    28681 gctgttgaag cagacattgc cgaactggac gacgaagaac gtgacgagtt tatgcaggag
    28741 cttgggctgg aagagccggg cctgaaccgt gtgatccgtg ccggttataa gctgctgaac
    28801 ctgcaaactt acttcaccgc tggggtgaaa gaagtgcgtg catggaccat tccggttgga
    28861 gcaaccgcgc cgcaggcagc gggcaaaatc catactgatt ttgaaaaagg ctttatccgt
    28921 gcacaaacca tctcgtttga agatttcatc acttacaaag gtgaacaagg cgcgaaagaa
    28981 gcaggcaaaa tgcgtgcaga aggtaaagat tacatcgtta aagatggcga tgtgatgaac
    29041 ttccttttca acgtctaatt aatttgttgt ctcatgaggt ttcgctaaat ctcatgaaga
    29101 gcgcaaaagt atatataatc cacgcaattg cgtggatttt tcatttcacc gagtcctaat
    29161 cattcttgta acatcctaca cttttgtgct tattgcctac agtgaaccac gagaataatt
    29221 aaagacatca gtgtcagatg acggatatct cattagtagt gatataacgt aagttttggt
    29281 ccactacaca tcaccccctt cacagctgat caatattgat cacgctccaa tatgctggcg
    29341 agaaaaggac ttacctgaag ggtaatatcc cgcagataaa gtccccgaat aacgtgatta
    29401 cgtgcgatcc tggcaagatg aaaatgataa ccagtagcac aataacaacg ataggccaga
    29461 aaacccattg accaaaaatg aatatcgatg ttttttgctt tttcttatta gtaatgcaaa
    29521 aatggcgcat attgtttttg ttatgca
//
LOCUS       DAZFYC010000055        29525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFYC010000055 DAZFYC010000000
VERSION     DAZFYC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.055"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(216..291)
                     /locus_tag="ACHKW5_002670"
     tRNA            complement(216..291)
                     /locus_tag="ACHKW5_002670"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Phe,seq:gaa)
     gene            complement(398..973)
                     /locus_tag="ACHKW5_002671"
     CDS             complement(398..973)
                     /locus_tag="ACHKW5_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7512040.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(1010..2707)
                     /gene="dsbD"
                     /locus_tag="ACHKW5_002672"
     CDS             complement(1010..2707)
                     /gene="dsbD"
                     /locus_tag="ACHKW5_002672"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO7512041.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(2683..3021)
                     /gene="cutA"
                     /locus_tag="ACHKW5_002673"
     CDS             complement(2683..3021)
                     /gene="cutA"
                     /locus_tag="ACHKW5_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO7512042.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(3137..4438)
                     /gene="dcuA"
                     /locus_tag="ACHKW5_002674"
     CDS             complement(3137..4438)
                     /gene="dcuA"
                     /locus_tag="ACHKW5_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO7512043.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(4556..5992)
                     /gene="aspA"
                     /locus_tag="ACHKW5_002675"
     CDS             complement(4556..5992)
                     /gene="aspA"
                     /locus_tag="ACHKW5_002675"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO7512044.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6329..6805
                     /locus_tag="ACHKW5_002676"
     CDS             6329..6805
                     /locus_tag="ACHKW5_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO7512045.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(6821..8077)
                     /gene="yjeH"
                     /locus_tag="ACHKW5_002677"
     CDS             complement(6821..8077)
                     /gene="yjeH"
                     /locus_tag="ACHKW5_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO7512046.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            8353..8646
                     /locus_tag="ACHKW5_002678"
     CDS             8353..8646
                     /locus_tag="ACHKW5_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO7512047.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            8690..10345
                     /gene="groL"
                     /locus_tag="ACHKW5_002679"
     CDS             8690..10345
                     /gene="groL"
                     /locus_tag="ACHKW5_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010427772.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO7512048.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMGGMM"
     gene            10483..10836
                     /locus_tag="ACHKW5_002680"
     CDS             10483..10836
                     /locus_tag="ACHKW5_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO7512049.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11039..11908)
                     /locus_tag="ACHKW5_002681"
     CDS             complement(11039..11908)
                     /locus_tag="ACHKW5_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7512050.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(12298..13326)
                     /gene="epmB"
                     /locus_tag="ACHKW5_002682"
     CDS             complement(12298..13326)
                     /gene="epmB"
                     /locus_tag="ACHKW5_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7512051.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            13368..13934
                     /gene="efp"
                     /locus_tag="ACHKW5_002683"
     CDS             13368..13934
                     /gene="efp"
                     /locus_tag="ACHKW5_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7512052.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            13986..14111
                     /gene="ecnA"
                     /locus_tag="ACHKW5_002684"
     CDS             13986..14111
                     /gene="ecnA"
                     /locus_tag="ACHKW5_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7512053.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            14222..14368
                     /gene="ecnB"
                     /locus_tag="ACHKW5_002685"
     CDS             14222..14368
                     /gene="ecnB"
                     /locus_tag="ACHKW5_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7512054.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            14550..14867
                     /gene="sugE"
                     /locus_tag="ACHKW5_002686"
     CDS             14550..14867
                     /gene="sugE"
                     /locus_tag="ACHKW5_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7512055.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(14864..15397)
                     /gene="blc"
                     /locus_tag="ACHKW5_002687"
     CDS             complement(14864..15397)
                     /gene="blc"
                     /locus_tag="ACHKW5_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7512056.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15486..16619)
                     /locus_tag="ACHKW5_002688"
     CDS             complement(15486..16619)
                     /locus_tag="ACHKW5_002688"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7512057.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(16682..17041)
                     /gene="frdD"
                     /locus_tag="ACHKW5_002689"
     CDS             complement(16682..17041)
                     /gene="frdD"
                     /locus_tag="ACHKW5_002689"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7512058.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(17052..17447)
                     /gene="frdC"
                     /locus_tag="ACHKW5_002690"
     CDS             complement(17052..17447)
                     /gene="frdC"
                     /locus_tag="ACHKW5_002690"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7512059.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(17458..18192)
                     /gene="frdB"
                     /locus_tag="ACHKW5_002691"
     CDS             complement(17458..18192)
                     /gene="frdB"
                     /locus_tag="ACHKW5_002691"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7512060.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(18185..19993)
                     /gene="frdA"
                     /locus_tag="ACHKW5_002692"
     CDS             complement(18185..19993)
                     /gene="frdA"
                     /locus_tag="ACHKW5_002692"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7512061.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            20318..21295
                     /gene="epmA"
                     /locus_tag="ACHKW5_002693"
     CDS             20318..21295
                     /gene="epmA"
                     /locus_tag="ACHKW5_002693"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO7512062.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            21514..23016
                     /gene="yjeM"
                     /locus_tag="ACHKW5_002694"
     CDS             21514..23016
                     /gene="yjeM"
                     /locus_tag="ACHKW5_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO7512063.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(23167..26490)
                     /gene="mscM"
                     /locus_tag="ACHKW5_002695"
     CDS             complement(23167..26490)
                     /gene="mscM"
                     /locus_tag="ACHKW5_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO7512064.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPIRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(26512..27480)
                     /gene="asd"
                     /locus_tag="ACHKW5_002696"
     CDS             complement(26512..27480)
                     /gene="asd"
                     /locus_tag="ACHKW5_002696"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO7512065.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEN
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(27577..28629)
                     /gene="rsgA"
                     /locus_tag="ACHKW5_002697"
     CDS             complement(27577..28629)
                     /gene="rsgA"
                     /locus_tag="ACHKW5_002697"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO7512066.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            28724..29269
                     /gene="orn"
                     /locus_tag="ACHKW5_002698"
     CDS             28724..29269
                     /gene="orn"
                     /locus_tag="ACHKW5_002698"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO7512067.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(29282..>29525)
                     /locus_tag="ACHKW5_002699"
     CDS             complement(29282..>29525)
                     /locus_tag="ACHKW5_002699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512068.1"
                     /translation="PDLGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSAT
                     PQKSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
BASE COUNT         7088 a         7479 c         7728 g         7230 t
ORIGIN      
        1 tgcgtcagtc agtgccatag ttcagccttc tgaatgcgtt aaagagggta caaattctgt
       61 cgaactgggg tatgcccgca attataccca cacaatgagg ttgatgtggg gagaatttgg
      121 ttgagttcgg tagaattgat ttggaggaag aacacttaaa tcatggtgtt ctgaaacgaa
      181 aaacggacct ccgtggaggt ccgttgatat gaatttggtg cccggactcg gaatcgaacc
      241 aaggacacgg ggattttcaa tcccctgctc taccgactga gctatccggg caacggggcg
      301 cattaaaccg taatctgcac atctcgtcaa cctaatttca gtaaaagcga ttcaactgct
      361 taagattgcg gcaaatcgct tcttttctgt gtttctgtca ggtcaatgca ccaccctggc
      421 ggcagcgggc gaagcgcagg atatcttccg ccagccgatg tgcagtgtcg acatccgcct
      481 ggctacgatt caccagcatt cggcttaaac agccttccag caccagttcc atctgctttg
      541 ctaccatcgc cggatcgtca acttccagcg tggttaacag ttcgtgggtg aaatcgtagg
      601 ccgcgctttt ttgctgatcg gccagttgat gaatagggtg gccaggatcg ggataaaacg
      661 tacaggcagc gataaataga cagcccggat agcggttgtt tttaacgcac tccgataacg
      721 cctgataacg tgccagcaac ttttgttcgg cggtttgcgt ttcgtccagc atcagctgac
      781 gacgccagac atctatctgt tggctaagat aacgcagcgc atcgtagagg attgcctctt
      841 tgtctggcca gaagcggcgt agctcgtcca gtggataatc cacacgttca gcaaccatct
      901 ccagcgtggt gttggcaatc ccttgtaatt ctaataattt cagggcttct cccagtacat
      961 cttcacgttg cacgctattt tcctccgtct ttcccactgc aagtgtcgtt cacggttggc
     1021 gatcgcgcaa atgtgcgctg aaggtttcag catccataaa gcccgtgacg cgtgcttgtg
     1081 gatgctcctg gccttgtccg tcaaaaaaga gaattgtcgg taggccaagg acattaagat
     1141 gctttaacag cgccacatct tgtgcgtcgt tggccgtgac gttggcctga agtaagaccg
     1201 tgtctgctaa cgctttttgc acctgcgggt cgctgaaggt gtatttctca aactctttac
     1261 aggcgacgca ccagtcggca taaagatcta acatcaccgg tttgcctttg gcttcaacga
     1321 gcgcctgatt taactcatct accgttttga tttgtgtaaa gttgagatgc gtctgagttt
     1381 gcgcggtatg cgtcgcacca aatgcccaat cctgaagtgg gcgcacgcta accaatgccg
     1441 ctgccagcag gattatttgc accacgcgca tccagccgcg tttagcctgt aggctggtga
     1501 taaaggccca gccaaagaat gcgacaccca gcgccgacca caagcgtaat ccccatacat
     1561 caccaatcac tcgctccagc aggaagaccg gcagtgcgag gatcacaaaa ccaaacgcgg
     1621 ttttgacttg ttccatccac gggccgcttt tcggcagcaa gcggttacca aagacggtaa
     1681 ttagcatcag cggcaggccc atgcccaacg catagagata aagcgtgccg ccgcccagcc
     1741 acatgttccc gctttgggcg atatacagca gaatcgcgct aagcggtgcg gtggtgcatg
     1801 gtgaacagat cagtccggca atcgccccca taacaaacac accgccaggt gagccgccct
     1861 gttggcgatt gctcatcaac gtgagacgtg tttgcagcga agaggggagt tgtagagtaa
     1921 acaagccaaa cattgacatc gccagcaagg taaagacgat ggcgaggcca atgagcacgt
     1981 atgggtgctg tagcgccgcc tggaactgta accctgcggc ggcaactacc agacccagcg
     2041 ccgtgtaagt cagtgccatc ccctgcacat aaataaaggt cagcaacaat gctctggcag
     2101 tggagagccg ctgtttacca cccagcacga tgccagaaat cagtgggtac attggcagca
     2161 cgcatggcgt aaaggcgata ccaataccga tcaacaacgc ccagagcgcg gaaaagggca
     2221 attgcgcggt gtgctgctct tgctgcgaaa cagacacagg ctgtgacgct gcgttgttgg
     2281 cgaccacttc gcttaacgga acggttttgg tttctggcgg ataacagaaa ccggcatcag
     2341 cacagccctg gtaggtgaca gttaacgtcg ctcccgcact cgcctggttg atggttacgg
     2401 gaagcgtcag ccgatcgcgg taaatctcgc ttttgccgta aaactcatct tcatgccaga
     2461 cgccttgtgg cagctgcacg tcggcaattt tcgcgtgttc cggcgtaatg cggatctgtt
     2521 tacggtagag gtagtaaccg tctttgatct gccaggtcag attaaggtca tgttggtttt
     2581 gctgaaaatc aaaagtaaag gcttgatccg cggggacaaa ttgtgaacgt cccggcgcgt
     2641 cgaataatcc ggcaaaaacg gaagtgctgc aaagtagcag gatcagcgta aagatgcgtt
     2701 gagccatgag aggtaatctg tgtctccgtg tgtaacaggt aaaaccagaa gttccggggt
     2761 ttgatatgga tgatgagact tcaggcattc cagcagtgcc tgctggtgag atacggtagt
     2821 ttttaaaatc atctgcactt cgtattcttg ctccagctta ccttcccagt aatagagaga
     2881 ggtagcgccg gggatcaagg tcgcgcaggc cgccagtttt tccgccagca ctttggcggc
     2941 taaatcctgg gctgtcgctt catccggtgc ggtacatagc accacgacag acgcggtatt
     3001 cgaacttttt tcatcaagca taaacacctc gcaagaacag atggaaccgc aagagagagg
     3061 tcactataca acggacgggg gaaggatgtt agtcggcggg aaagaaagcg gggcgtgaac
     3121 gccccgcgat tggtcattac agcatgaagc tacccagcac gaagccgaag caaacggcca
     3181 gggcaacacc cagagtaccc gggatgaaga acggatggtt gaagacgaat ttaccgatac
     3241 gggttgtacc cgtgtcatcc atctgtaccg cagcaaccag cgtcgggtag gtcggcagaa
     3301 tgaacagacc agacaccgca gcgaaagaag caacagcggt cagcggtgaa acgttcagtg
     3361 ccagggccat cggcatcagt gcttttgcgg ttgcagcctg agagtacagc agagcagaag
     3421 caaagaagaa gatgacggcc agcagccacg gatgaccctg aatcacttca ccagcggtat
     3481 ctttgatcca gtcgatgttg ttggaaacga aagtatcgcc cagccacgca acacccagga
     3541 tacaaataca ggcgctcata cctgctttga aggtgctgga gttgaggatg ttgtcggtat
     3601 cgactttaca gataacggtg gtcagagttg caacgctgag catgatgatc aggattgcgt
     3661 tggtggtgtt catcagcggt ttttcaacca gacccatgct tgggctgttg atgattgcat
     3721 agataaccac gccaactacg cccagcagga acagccagac ggacgttttt gcaccggatt
     3781 tgatttcaat ctgcttttca ccgcgcagtt caaccaggcc ctcttccaga cgcttgcgat
     3841 aaatcggatc gtcagagagt ttggagttga atagcatagt gaccagaaag gacatcacca
     3901 gaaccgccag cagggtggac gggatgacca cggagagcag atggaggtag ctgatgccat
     3961 gaccttccat cacggaagac atgtaaacca ctgccgctga gattggcgat gcggtgatcg
     4021 caatctgcgc ggataccact gcagtagaca gcggacggca aggtttgacg ccttgttcct
     4081 tcgcaacttc agcgataact ggcagtgtcg ccagagatat gttgccagta ccagcaaaga
     4141 tagtcaggaa ataggtcacg atcggtgcga ggatcgtgat gtatttcggg ttacggcgca
     4201 gcagcttttc tgtctgatga accagatagt ccagaccgcc agcaacctgc atggcagaaa
     4261 tagcggcgat aaccgccatg ataatggaaa tgacatcgaa cgggatgtta ccgggtttaa
     4321 cgccaatagc ggcaagaacc agcaccccca atccgcctgc aaaaccaata cctattcccc
     4381 ccaatctggc gcccaagaag atcgccagca aaactatgat gagttctaca actagcatat
     4441 tagccttcct tgttttttaa caagttgata ttaggttgtt atttttaagt tactgctcac
     4501 aagaaaaaag gcacgtcatc tgacgtgcct tctttatttg tactaccctg tacgattact
     4561 gttcgctttc atcagtatag cgttttgctt tgtaagccgg gtgcatcaga ttctgtacgg
     4621 agaaaatatc gtcaagttcc gcttcagtca acagaccgcg ttccagaacg acttcacgta
     4681 cactcttacc ggtttcggca cagattttac ccacgatgtc accgttgtgg tgaccgatga
     4741 acgggttcag gtaagtaacg ataccgatag agttgtaaac gtaaccttcg cacacttctt
     4801 tgttagcagt gatgccgtta atgcattttt ccagcaggtt gtagcaagcg ttggtcagaa
     4861 tgtgaacgga ttcgaacatg gcctggccaa tgaccggctc cataacgttc aactgcagct
     4921 gacctgcttc tgctgccatg gtaacagtgg tgtcgttacc gatgactttg aagcatacct
     4981 ggttaaccac ttccggaaca accgggttta ctttagctgg catgatggaa gagcccgcct
     5041 gcagttccgg caggttgatc tcgttcaggc cggcacgtgg accagaagag agcaagcgca
     5101 ggtcgttaca gattttggac atcttcacag ccaggcgttt cagcgcgccg tgaaccataa
     5161 cataagcgcc gcagtcagag gtcgcttcga tcaggtcttc agccggtacg catgggaagc
     5221 cagtgacttc agccagtttt ttcactgcca gcggagagta ctctttcggc gtgttcagac
     5281 cagtaccgat tgctgttgcg ccaaggttaa cttccagcag cagttcagcg gtacgttgga
     5341 tatttttcac ttcttctttc agcaggatgc tgaaagcgcg gaattcctga ccgagggtca
     5401 tcggtactgc gtcctgcagc tgggtacgac ccattttcag gatgtcctgg aattcgacag
     5461 ctttacgttc aaagccttca cgcagttggt taatcgcatc taccagctta atcagggaag
     5521 agtaaactgc gatacggaaa ccggtcgggt aggcgtcgtt agtggactga catttgttaa
     5581 catggtcgtt cgggttcagg tactgatatt cacctttctg gtgacccatc agttccagac
     5641 cgatattggc cagcacttcg ttggtgttca tgtttacgga agtacctgcg ccgccctggt
     5701 agacgtctac cgggaactga tccatgcatt ttccgttgtt caggacttca tcacatgcgg
     5761 caatgatggc attcgctaca cttttaggaa tggtttgcag ctctttgttt gccatagctg
     5821 cggctttttt aaccattacc ataccgcgaa caaattcagg aatatcactg attttgttgt
     5881 tgctgatata gaagttttca atcgctctca gagtgtgaac accatagtag gcatcagctg
     5941 gaacttccct ggtacccaac agatcttctt cgatacgaat gttgtttgac atgtgaacct
     6001 tctttttcaa gctgccaatg atttgcttta aacacacaga atatatgtgg tttcgaatgt
     6061 ttttcgaccg acgattatcc cctgcatcga ccgaataccc gagatcatat gctgcttgag
     6121 gatttctacc gtaatctgga tcactttaag tgtcggtttt taccccttaa ttattaattt
     6181 gtgaaataga tcaccgcttt gggattacta ccaaaaatag ttgcgcaaac atcttgaaat
     6241 tttgctaatg accacaatat aagctgaacg cgattcgcaa cccattcagg tagccgggtt
     6301 ttaccggctg ctattacagg agaaaccttt gcgctggtta ccttttattg ccattttcct
     6361 ttatgtctat attgagattt caatctttat tcaggttgcc catgtattgg gggtattgct
     6421 gaccctcgtg ctggttatat tcacgtcagt tatcgggatg tcactggtac gtaaccaggg
     6481 ctttaagaat ttcgtgctga tgcagcaaaa aatggcggcg ggtgaaaacc cagcggcgga
     6541 gatgattaaa agtgtttcgc tgatcattgc cggtttgctg cttttattac cgggcttttt
     6601 caccgacttc ctcggtcttc tgcttttatt accgccggtg caaaagcatc tgacagtgaa
     6661 gttgatgccg catttgcgct tttctcgtat gccaggcggc ggttttagcg ccgggaccgg
     6721 tggcggtaat acttttgatg gtgagtacca gcgaaaggat gatgagcgcg accgccttga
     6781 tcataaagac gatcgccagg attaatgtcg aaacgccgga ttatgtggtt atgccatttt
     6841 ccggcgtttt tcgttttggc agcaacagcc ataaccccgc cagcatgatc agcgcataga
     6901 gacttttcca gccgaccatt gccagtaaca gaacgcataa cagcccgcca accaccgcca
     6961 gtagtcgata acgtccttgc aataatttac agcctgccag catgcataac agataaatca
     7021 taataaagat gccattggca taaataataa gagcgtccag attgatctct aaagcatgaa
     7081 tcaccaacgt gctcaccaca cagcagccga gcaccgcatt gagggcatta ttcgggatat
     7141 ggcgagaaga gaggcgtgcc aggtagtggt caggattatg ttgcgcctgc gaccagacca
     7201 ggcgggcgaa gctctgtata taaatgttga gactggcaaa gcaggccaga tagccaatca
     7261 cgcaggcaat ccataacgct cctacaccga acaactgcac tacaattttt ggaagcgatg
     7321 ctgccgccgc cattttttca ccataggcgt cgaagtgtaa gacgactacc gtacagcccc
     7381 agtagactaa tcctgccagc agcagaccaa tcatcaatgc gcgaggaaaa tcacgctctg
     7441 gatttttaaa ttccgaggca agatgggcaa atgcttccag accgacaaaa caccagaaca
     7501 tcactgataa cgcagcaaat aacccggtaa gttcgatatt accaggtgcc gggaagggga
     7561 tattcgcagg tttgatatcg cccgcccacc agatagcgac aatcagcgcg acgataagtc
     7621 cggcaataac ggtttgtaga ttagcactgg aactggcacc gcgagtaccg atataccaca
     7681 ccagcgccag cgtaccgagt tctgccaaca acagttgcca gctatgccag ccaaacattg
     7741 cctggccgaa cccggcggca atttgtagcg cggcaggcaa acccacggga atgaccgata
     7801 aaaacaacca gccggtgact cgctcaagtc gcgaaccaaa cgccataccg acgaagtgcg
     7861 cgacaccgcc tgcgctggga tagtggcgac ccagaatcgc aaacacaatc gcaatcggga
     7921 acactaagat aatcaaaacg ggccacgccc acaggctgtt attgcccgct accagcgcag
     7981 ctaacgcagg aacggcaaac acgccagtgc ctaataatga cgtcgatagc aggccaatgc
     8041 cttgggccag ccccagttct tgtttgagtc cactcatggg ttgatatccg attgcgccca
     8101 aattttgggc aactgcgtag attttcgatg gtagcacaat cagattcgct tatgacggcg
     8161 atgaagaaat tgcgatgaaa tgtgaggtga atcagggttt tcacccgatt ttgtgctgat
     8221 cagaattttt tttctttttc ccccttgaag gggcgaagcc tcatccccat ttctctggtc
     8281 accagccggg aaaccacgta agctccggcg tcacccataa cagatacgga ctttctcaaa
     8341 ggagagttat caatgaatat tcgtccattg catgatcgcg tgatcgtcaa gcgtaaagaa
     8401 gttgaaacta aatctgctgg cggcatcgtt ctgaccggct ctgcagcggc taaatccacc
     8461 cgcggcgaag tgctggctgt cggcaatggc cgtatccttg aaaatggcga agtgaagccg
     8521 ctggatgtga aagttggcga catcgttatt ttcaacgatg gctacggtgt gaaatctgag
     8581 aagatcgaca atgaagaagt gttgatcatg tccgaaagcg acattctggc aattgttgaa
     8641 gcgtaatcct cgcacgacac tgaacatacg aatttaagga ataaagataa tggcagctaa
     8701 agacgtaaaa ttcggtaacg acgctcgtgt gaaaatgctg cgcggcgtaa acgtactggc
     8761 agatgcagtg aaagttaccc tcggtccgaa aggccgtaac gtagttctgg ataaatcttt
     8821 cggtgcaccg accatcacca aagatggtgt ttccgttgct cgtgaaatcg aactggaaga
     8881 caagttcgaa aatatgggtg cgcagatggt gaaagaagtt gcctctaaag cgaacgacgc
     8941 tgcaggcgac ggtaccacca ctgcaactgt actggctcag gctatcatca ctgagggtct
     9001 gaaagctgtt gctgcgggca tgaacccgat ggacctgaaa cgtggtatcg acaaagccgt
     9061 taccgctgca gttgaagaac tgaaagcgct gtccgtaccg tgctctgact ctaaagcgat
     9121 tgctcaggtt ggtaccatct ccgctaactc cgacgaaacc gtaggtaaac tgatcgctga
     9181 agcgatggac aaagtcggta aagaaggcgt tatcaccgtt gaagacggta ccggtctgca
     9241 ggacgaactg gacgtggttg aaggtatgca gttcgaccgt ggctacctgt ctccttactt
     9301 catcaacaag ccggaaactg gcgcagtaga actggaaagc ccgttcatcc tgctggctga
     9361 caagaaaatc tctaacatcc gcgaaatgct gccggttctg gaagctgttg ccaaagcagg
     9421 caaaccgctg ctgatcatcg ctgaagatgt tgaaggcgaa gcgctggcaa ctctggttgt
     9481 taacaccatg cgtggcatcg tgaaagttgc tgcggttaaa gctccgggct tcggcgatcg
     9541 tcgtaaagct atgctgcagg atatcgcaac cctgactggc ggtaccgtaa tctctgaaga
     9601 gatcggtatg gagctggaaa aagcaaccct ggaagacctg ggtcaggcta aacgtgttgt
     9661 gatcaacaaa gacaccacca ctatcatcga tggcgtgggt gaagaagctg caatccaggg
     9721 ccgtgttgct cagatccgtc agcagattga agaagcaact tctgactacg accgtgaaaa
     9781 actgcaggaa cgcgtagcga aactggcagg cggcgttgca gttatcaaag taggtgctgc
     9841 taccgaagtt gaaatgaaag agaaaaaagc acgcgttgaa gacgccctgc acgcgacccg
     9901 tgctgcggta gaagaaggcg tggttgctgg tggtggtgtt gcgctgatcc gcgtagcgtc
     9961 taaactggct gacctgcgtg gtcagaacga agaccagaac gtgggtatca aagttgcact
    10021 gcgtgcaatg gaagctccgc tgcgtcagat cgtcctgaac tgcggcgaag aaccgtctgt
    10081 tgttgctaac accgttaaag gcggcgacgg caactacggt tacaacgcag caaccgaaga
    10141 atacggcaac atgatcgaca tgggtatcct ggacccaacc aaagtaaccc gttctgctct
    10201 gcagtacgcg gcttctgtgg ctggcctgat gatcaccacc gagtgcatgg ttaccgacct
    10261 gccgaaaaat gatgcagctg acttaggcgc tgctggcggt atgggcggta tgggcggcat
    10321 gggtggcatg ggcggcatga tgtaattgcc ctgcacctcg cagaaataaa caaacccccg
    10381 ggcagaaatg tctgggggtt tttcttttgg tcatctttct agtataagat tcagacacgg
    10441 acgacgcgag tggcgtccag ctcattgatt atggggaata acatgcacgt aaaatactta
    10501 gcagggattg tcggtgccgc gctactgatg gcgggttgta gctccagcaa cgaattgagt
    10561 gctgccggtc agagtgtacg cattgtggac gagcagccag gcgcagagtg ccagctgatt
    10621 ggtactgcga caggtaagca aagcaactgg ctttccgggc aacacggaga agagggcggt
    10681 tctatgcgcg gcgcagcaaa cgatctgcgc aaccaggcgg ctgcaatggg cggtaacgtg
    10741 atttatggca tcagcagccc gtcgcaggga atgttgtcca gttttgtccc gacggatagc
    10801 cagattatcg gtcaggttta taagtgcccg aactgatgtg atccagaggc tgggtgacaa
    10861 acgtatcatt gcctgatgcg cttcgcttat caggcctaca gcaggcattg caactgattg
    10921 aatgcttgag aatttgtagg ccggataagg cgtttacgcc gcatccggca tgaccaacga
    10981 tcacgttatc agtcatttca ggctggatgg ctccagcctc gcattagcaa agatgagatt
    11041 atttcgcctg tggtgcagtt ttgctggtgg attgaacacg taagtgatgc agcgcgcgta
    11101 agcattgatc ctgggttagc ggttgggcgt tattcgccgc tacggtgcga acatagacct
    11161 gacatggcac acccgcgagt tttttcagtc gtgggctgaa atcaagcatt cggcgagtga
    11221 tcgcctctat ttctttaccc ggcgttgctt cacgcgtttt caccactgcg ccgctaaatt
    11281 gttctttgcc actttcatct ttaaagcggt aaagcaccgc cagataatgg tcgaacaggt
    11341 tatgtttcca ttccggttgg gaataggtgt catattccag attgccattt tcctggcagt
    11401 caacgtcata taacggcagg aaatctaaca gtgaggtaat acgtaatgcc agttcacgca
    11461 tttccgcatt attaattgca tgaatgattg ctcgcgccag catttcaatc tgcaactcat
    11521 tgacgactaa ctcacatttg ctaccgtcat gtagcgttaa tacgaaggta agattttcgc
    11581 cttgattatc ggtaagttcc agcgtgttaa cccgacgatt aatatcggca tttttaagct
    11641 catcaaccag aatttctggg atgttttctg ccattttttt aatgacttta tcgcgagctt
    11701 gctcatactg aaggcgtgca gcagcatcca gtttatgctt ttgatgcata cgactttcca
    11761 gcgcaatcag caggtcgcgc agttccatga cagacataaa gaacaacgat tctttattgc
    11821 gaggttcttt aattttcagc gccagtgcaa tgaaattatt actcttgcga ataacaccgg
    11881 tattaacacc cttgatgctt atggccatgt aggttctccc taaccatttc tcaattaaat
    11941 aattaatttt aatttataag ccagataaat gggcttggta gtaatagttg ttaaaataac
    12001 ataaatagcc gtgctcactc tatataaaca gaaaagaaag gttaattatt ggtgttagct
    12061 atataaaaag taacaacagc aatgcatatg aataattaaa ggttatgcaa cgtgcaaaga
    12121 ttaaatttcc cagtttaatg acaggttgct gtacttgcgt ttgttgctct tgccggatgc
    12181 ggcgtaaacg ccttatccgg cctacagttc attgcaaatc cattcaattt caatgcacaa
    12241 tgtaggcctg ataagcgtag cgcatcaggc taatgtttaa cttccctgtt taatcagtta
    12301 ctgctggcgt agctggagat ccagcggcgt tttgctgggt tcgccgccga tttctcgcgc
    12361 cagtttcggc accagataac ccgacaccag tgtcagcaac tcacgcataa tctgccgtgc
    12421 ttcgtcatca ctcaccataa aatgcgccgc gccctgtact ttatcgagca catgcaggta
    12481 atacggcatt acgccggcat cgaacaacgc attactcagg tttgccagcg tttgtgcgtt
    12541 atcgttcaca ccacgtaaca gaacgctctg gttaagcagg gtgacaccga cacggcgcaa
    12601 tttagccatc gcctgacgga acgtttcatc tacctcattg gcatgattga tgtgattcac
    12661 cagcaagatt tgcagcgtag aacgggcaaa gcgttcaacc agcgcctcgg tgatacgtgc
    12721 cgggatcaca atcggcagac ggctgtgaat ccgcagacgt ttgatatgcg ggatggcttc
    12781 cagttgtgtg agcaaccagt ccagctcgtg atctttcgcc atcagcggat cgccgccgga
    12841 gaaaatcatc tcgtccagtt ccggatgcgc agcaacatac tcaagagcag tctgccagtt
    12901 acgcttgttg ccctgatttt cggcataggg gaagtggcga cggaagcaat agcggcaatt
    12961 taccgcgcag ccgcctttga ccagcaaaag cgcccggttg tggtatttat gcaacaaacc
    13021 aggcactacg ctgtgctgtt cttccagcgg gtcggtggag aatccggacg cgacgacaaa
    13081 ctcatcttgc gaggtaagta cctgacgcaa aagaggatcg tccggattgc ctttctccat
    13141 gcgatcgata aatgagcggg gcacacgcag ggcaaacagc tttttggcgc tgcgtccggc
    13201 caacagtttt tcgtccgcgt ctatattcaa aagacgcaga agttcatcag gatcggtcac
    13261 aacatcggca agttgcgtta accaatcttc tctggatggg gtatttaggg ttacaatatg
    13321 cgccattttg tggcttagct accaattaac aaatttcaga gggccttatg gcaacgtact
    13381 atagcaacga ttttcgtgct ggtcttaaaa tcatgttaga cggcgaacct tacgcggttg
    13441 aagcgagtga attcgtaaaa ccgggtaaag gccaggcatt tgctcgcgtt aaactgcgtc
    13501 gtctgctgac cggtactcgc gtagaaaaaa ccttcaaatc tactgattcc gctgaaggcg
    13561 ctgatgttgt cgatatgaac ctgacttacc tgtacaacga cggtgagttc tggcacttca
    13621 tgaacaacga aactttcgag cagctgtctg ctgatgcaaa agcaattggc gacaacgcta
    13681 aatggctgct ggatcaggca gagtgtatcg taactctgtg gaatggtcag ccgatctccg
    13741 ttactccgcc gaacttcgtt gaactggaaa tcgttgatac cgatccgggc ctgaaaggtg
    13801 ataccgcagg tactggcggc aaaccggcta ccctgtctac tggcgctgtg gttaaggttc
    13861 cgctgttcgt acaaatcggc gaagtcatca aagtggatac ccgctctggt gaatacgtct
    13921 ctcgcgtgaa gtaatgcggt tgtggtgcgg cctgcaggct gcaccatcac ttattcaggt
    13981 cagagatgat gaaacgcctt atcgttcttg ttttgcttgc cagcacgctg ctcacgggct
    14041 gtaacaccgc tcgcggtttc ggcgaagaca tcaaacatct cggcaactcc atctctcgcg
    14101 ctgccagcta atttttcttc tcttccgaaa aatcatcaga tttccgtcat ttttggcgat
    14161 gttgtctatt attaatttgc tataggcaaa cataaataac attacctaaa aggaagacgt
    14221 tatggtgaag aagacaattg cagcgatctt ttctgttctg gtgctttcaa cagtattaac
    14281 tgcctgcaac accacgcgtg gcgttggtga agacatttct gatggcggta acgcgatttc
    14341 tggcgcagca acgaaagcgc agcaatagca ataagcaata acggtacgac aggtatgtcg
    14401 tgccgtttgt tttttctgcg atagtcacta aggtaatagt tgaaattccc ctgccacctg
    14461 gcaaaatatc cgttcaacca tcagctttgc aggacgacct gcaaacgcct cttttcaccg
    14521 gggacggccc caattctccg gagcctgata tgtcctggat tatcttagtt attgctggtc
    14581 tgctggaagt ggtatgggcc gttggcctga aatataccca cggctttagt cgtttgacgc
    14641 cgagtgttat taccgtgacg gcgatgattg tcagtatggc gctacttgcc tgggcgatga
    14701 aatcgttacc agtagggacg gcttatgccg tgtggacggg tattggcgca gtcggcgcgg
    14761 ctatcaccgg cattgtgctg ctcggtgagt ccgctaaccc gatgcgcctg gcgagtctgg
    14821 cgttaatcgt attggggatt attggtctga aactcagcac tcactaacta ccgggctgct
    14881 gtacccaaat aaacttactg acatcaaacc cttcccgggt cgcgactgcc agcatctcct
    14941 gtttcacttc gtcagaaatg gttggcgtgc gggagagtat ccacaggtag tcgcggtccg
    15001 ggccgcaaac cagcgcatgg cggtattccc gatcgagtgc aataacgtta taaccgccat
    15061 agaaaggacc aaagaatgac actttcagcg cagcgcgagt tggtgcgccg gtaaagtacg
    15121 ctttcccttc actctgctgc cacattcctc tgtcagggtt atagccttta ttaatgacat
    15181 tcaggccgcc gtcatcacgc aggctgtatg ttgcggtgac tttttccagt ccacgttcaa
    15241 aacggtgatc aaaacgggca atctcatacc aggtaccgag ataacgtttg gcgtcgaaat
    15301 tattcactac tgttacgcca cgcggcggcg taggagaact gcaggcaacg accagaaatg
    15361 cagctgtcgc tgcggcaacg agagggagca ggcgcataaa tgtttcctta ctggtttttt
    15421 tctaagtgta gatgacagca aggaaaagcg cagaaaaggt ccgaaaattc ggacccgatg
    15481 aaattttact gtagagcgtt gagaatctgc caggcggcgg cgactctcgc tggattggga
    15541 tagtttttgt tagccagcat cactataccc agctcttttt ctggaataaa cgcgacatag
    15601 ctaccaaatc cgccggttgc ccctgtttta tgtacccatg atgcgcgtac tgcaggagtt
    15661 gggggcgtaa tcgcttttac ggggtgtgct gccagtgcaa ttttattgcc actgccgtta
    15721 atgatgctgt caggatttac cggccagtcc agcatttccc agcccaggcc ctgatacata
    15781 tcgccggttt gccagtagcg agattgtgcc agttgtatcc cttgctgaag tgttttgtcg
    15841 ttgatatcac ggggattcat attgcttcgt acccagcagg ccatatcttc aatggtcgac
    15901 ttcacaccat aagtttcagc atctaacgcc cctggcgaaa catgaactgc cttgccttcg
    15961 cgatatcccc aggcgtaatt cttttcttct gcgggcggta cattaatcca cgtatggttg
    16021 agtttgagtg gctggaagac acgtgtttgc atcgcctgct caaaactcaa accagacggc
    16081 ttcacagcca gtgcgccgaa caaaccgata ctggagttgg catacagacg ttgtgttcct
    16141 ggcgcccatg caggctgcca gttttgatag aagcgcagca agtcgcttga ggatttcacc
    16201 tcatccggca cctgcaatgg caggccgcca gcagtgtagg ttgcgagatg taatagtgtg
    16261 atcccattcc actgtttagc ggtaagttca ggccagtatt ttgttgtggg atcgcttaac
    16321 ttgatttccc ctcgagcaat agcgtcgcca ccaagcacgc ccgtaaatgt tttgctgacc
    16381 gaacctaact caaacaacgt ttgctgtgtg acgggctgct ttttggcgat gtccgcatag
    16441 ccccaggtaa agtaataagg tttaccctga taaattaccg ccaccgccat accggggatc
    16501 ttttgttgct ctataagcgg ggtaattgtg cgatgcacaa tatcgttgat ttgttgaggg
    16561 gcagcaaatg tggagcaaga ggcggtaatt aataaggcgc agagcgtcgt tttgaacata
    16621 aggtctggtt tccatacaaa acggcccgcc ataggcgggc cggatttaca ttggcgatac
    16681 gttagattgt aacgatacca atcagcgtga caactgtcag gatagcagcc agaccgtaga
    16741 aaacccattt gcccgcaggc acgtgaattt tcagatcgtg catcgcgtgg tgcatacggt
    16801 gtaaaccaca ccacagcggc agaacgatca tcaggaacag gaatacgcga ccaatgaagc
    16861 tctgcgcgaa cgccagaacg cgctcgtagc tcagcgcatc acccggaaac agccccagtg
    16921 gcagcagaat acccaccagc aggatcatca ccggcgcaat aattgcgctc cacataccac
    16981 cggccccgaa gaggccccag aataccggtt cgtcagaacg ctttggattt ggattaatca
    17041 tctcaggctc cttaccagta cagggcaaca aacaggatta cgatggtggc aaccacagtt
    17101 accgcccaga gacttttgat aattggctct ggtcccattt tttcgtcttt tacaatgata
    17161 ttggccgctt tcggtgccag ttcaaaccag gttttggtgt gcagcagagc tgccgccaga
    17221 gtgatcaggt taatgatcac gataaccggg ttttgtaaaa agtcgacgaa tcccgcccag
    17281 gcttccgggc catttttcag ggcaaacagc ccgaaaatca gttcaatgct gaaccacaca
    17341 gccggaaccg ccgtgccttc gcgcagcatg taaaagcgat aaaacggcaa ttttttccac
    17401 caggtggacg tcattggccg tacatacggt ttacgtttag tcgtcatgtt gcactcctta
    17461 gcgtggtttc agggtcgcga taagaaagtc tttcgaactt tctactttgc cctgctgaat
    17521 ggccgcagcc ggatcgacgt gtttcgggca gacttcggag caatagccca cgaaagtaca
    17581 gctccatacg ccgttctggc tgttcaactg cgccatacgc tccttcttac cgtggtcgcg
    17641 gctatcttcg ttataacgat gcgccagcgt aatggcagcc ggaccgatga actctgggtt
    17701 caggccaaac tgcgggcacg cggcgtagca cagaccacag ttgatgcaac cggagaactg
    17761 gtgatacttt gccatctgcg ccggggtctg gatgttagta ccctgatccg cggtgcggga
    17821 gttgccgatg atgtacggtt tgatcgcttc cagactttcg atgaagtggg tcatatcgac
    17881 caccagatcg cgttcaatcg ggaagttagc taacgcttca accttcatac cgtcggtgta
    17941 atcacgcagg aaggttttac atgccagttt tggcacgttg ttaaccatca tgccgcagga
    18001 accacaaatc gccatacggc aggaccagcg gtaactcagg tccggtgcca ggttgtcttt
    18061 gatgtagccc agcgcatcca gtaatgaggt agttgcgtca taaggcactt catagaatgc
    18121 gctatgcggt gcggtatcga cttccgggtt atagcgcacc acctcaattt tcaggttttt
    18181 catctcagcc attcgccttc tccttcttat tggctgcttc cgccttatcg gctgcatccg
    18241 cttcgccacc gtaaacgcgt ttagctggcg gcagcgtagt aatcttcacg tcgctgtact
    18301 ccaggcgagt cgtgccatca gcatcgcgga aggcgagggt gtgtttgagg aagttgacgt
    18361 cgtcacgctc ggtgcaacct tcgtccagac gctggtgtgc gccgcgggac tctttacgtg
    18421 ccattgcgga gtgcgccata cattcagcaa cgttcagacc gtggcccagt tcaatggtgt
    18481 agagcaggtc ggtgttgaac acgctggagg tgtcggtgat gcgcacgcgc ttgaagcgtt
    18541 cctgcagctc tgccagcttg tcgatggttt tctgcatcag ttccggcgta cggtagatac
    18601 cgcaaccttc ttccattgcc aggcccattt cgtcgcggat cttcgcccag ttttcgccgc
    18661 catcctggtt aaccagatct ttcagacgtt gttcaacgcc agctgcctgc gcttcaattg
    18721 ccgcttcgtt gccattaccg gcagttgctg cacgctctgt cgcttgttca ccggccagac
    18781 ggccgaagac caccagttcc gccagggagt tagaacccag acggtttgca ccgtgcagac
    18841 caacagagga acattcaccc acggcgaaca gacctttaat gcgggtttca cagttctgat
    18901 cggtttcgat accgcccatg gtgtagtgtg cggtcggacg taccggaatc ggttctttaa
    18961 ccggatcgac gccaacgtac gctttcgcca gttcgcagat gaacggcaga cgttcatgca
    19021 gttttttctc gccgaggtga cgcaagtcga gataaaccac atcgccacgc ggcgtggaga
    19081 tggtgttgcc tttacgccat tcgtgccaga aggcctgaga aactttgtcg cgtggaccca
    19141 gttccatata tttgtttttc ggctcgccca gcggagtttc cgggcccatg ccgtaatctt
    19201 gcagataacg gtagccattt ttgttgacca gaataccgcc ttcaccgcgg cagccttcgg
    19261 tcatcaggat accggaacct ggcagaccgg ttgggtgata ctgaacgaat tccatgtcac
    19321 gcagcggaac gccgtggctt agcgccatac ccataccgtc accggtaacg atgccgccgt
    19381 tggtgttgta acgataaacg cgacccgcac cgccagtagc cataacgacc gcgttagcac
    19441 ggatctgcac cagcgtgcct tccatcatgt tcattgctac cacgccgcga acatgaccat
    19501 catcaaccag aatatccagc acgaaatgtt cgtcaaaacg ctggatctgc gggaattgca
    19561 gagaggtctg gaacagcgtg tgcagcatat ggaagccggt cttatcggcg gcgaaccagg
    19621 tgcgctcgat tttcatgccg ccgaagcgac gtacgttgac gctaccatcc gggcgacggc
    19681 tccatgggca cccccacagt tccagttggg tcatttcggt tgggcagtgg tggacgaaat
    19741 aatccacgac atcctgctca cacaaccagt cgccacccgc tactgtatcg tgaaagtgat
    19801 attcgaagct gtcatgatcc tgcgcgacag cggcggagcc cccttctgca gcaacggtat
    19861 ggctacgcat cgggtatact tttgagatta gtgcgatttt tgcatttgga tttgcctgcg
    19921 cggcagcaat tgcagcacgt aatcccgcgc caccggcgcc tacaatggca agatcggctt
    19981 gaaaggtttg cacgacattc ctccagattg tttttatccc acagccccgt acttcagggt
    20041 aagtgcctga aagttacggt ctgcgaacgc tattccactg ctcctttata ggtacaacag
    20101 tatagtctga tggataagtc tgaaatttga cgagatcgat ttttttagtg cgcaagaggg
    20161 ctaaattatc actgaagatg attaatttaa ttactaaacc atcagatcgt gcttttttta
    20221 gtcactgccc accgctgttt gattcctgcg taaaatttgt ctcgccgtcg cgttgcgagt
    20281 agacttcgtg cccttgtcaa aaactggaga tttaactatg agcgaaacgg catcctggca
    20341 gccgagcgca tccattccta acttattaaa acgcgcggcg attatggcgg agatccgtcg
    20401 tttctttgcc gatcgtggag tgctggaggt ggagacgcct tgtatgagcc aggcgacggt
    20461 aaccgatatt catttggtcc cgtttgagac acgtttcgtt ggccccgggc attcgcaggg
    20521 gatgaatctc tggttaatga ccagcccgga ataccatatg aaacgcctgc tggttgccgg
    20581 ttgtgggccg gtattccagc tgtgccgcag cttccgtaat gaagagatgg ggcgttatca
    20641 caaccctgag ttcactatgc tggagtggta tcgaccgcac tatgatatgt accggttgat
    20701 gaacgaggtg gacgatctct tacaacaggt gctggactgc ccggcagcag aaagcctttc
    20761 ttatcaacaa gctttcttgc gttatctgga aattgacccg ctctctgccg acaaaacgca
    20821 actgcgggaa gtcgcagcga aactggattt gagcaatgtt gctgataccg aagaagaccg
    20881 cgacacgctg ctacaattgc tgtttacctt tggcgtagag ccaaatattg gcaaagaaaa
    20941 accgaccttt gtgtaccact ttccagccag ccaggcatca ctggcgcaaa tcagtaccga
    21001 agatcatcgg gtcgctgaac gctttgaggt ttattataaa ggtattgagc tggcgaatgg
    21061 tttccatgaa ttgacggatg cccgtgagca gcaacaacgc tttgaacaag ataaccgtaa
    21121 gcgcgcggcg cgcggtttgc cgcagcaccc cattgaccag aatctgattg aagccttgaa
    21181 agtcggtatg cctgactgtt ccggcgtggc attaggtgtt gatcgtctgg tgatgttggc
    21241 gctgggcgcg gagacactgg ctgaagtcat cgcctttagc gttgaccggg cataattctg
    21301 aaaaattact gaattaacag cgaagaatgg cgtgataagc gccattctgt agcatatgtt
    21361 ttcatttttc cgttaaaggc cgcaatttaa gactggatat ttgatatcat ccaggtatca
    21421 attctgtatt ggtttttacg ctgcctgctc aatgttgcgc agtgcagttc agtgggcacg
    21481 tgttatacac gcgctgaaat gaaggatggt ttcatgcctc acacgataaa aaagatgagt
    21541 ctgataggac tcatattgat gatctttact tccgtatttg gatttgccaa tagcccatcg
    21601 gcttattact taatgggtta tagtgcgatt cccttttata tattttctgc attgttattc
    21661 tttattccat tcgccttaat gatggctgaa atgggagctg cttatcgcaa agaagaaggt
    21721 ggtatctatt cctggatgaa taatagtgtc ggaccacgtt ttgccttcat tggtacgttt
    21781 atgtggtttt cctcttatat catctggatg gtgagtacct ccgcgaaagt ttgggtaccg
    21841 ttctcaacat tcctctatgg tagcgacatg acccagcact ggcgtattgc cggactggag
    21901 cctacgcagg tggttggtct gctggcagta gcatggatga ttctggtcac cgtcgttgct
    21961 tcaaagggga ttaataaaat tgcccgcatt actgcggtgg gcggtattgc agtaatgtgt
    22021 ctgaatttag tattgctgtt agtaagcatt actattttgt tattaaatgg tgggcatttc
    22081 gcgcaggata ttaatttcct tgcatcaccg aacccaggtt atcagtccgg tctggcaatg
    22141 ctatcgtttg tggtatttgc tatttttgcc tatggcggaa ttgaagcggt tggtggtctg
    22201 gtcgataaaa cggaaaatcc agaaaagaac tttgccaaag gtattgtttt tgccgctatt
    22261 gttatttcaa tcggttattc gctggcaata tttttatggg gtgtcagcac aaactggcag
    22321 caggtattaa gtaatggttc cgttaacctc ggcaatatta cctatgtgct gatgaagagc
    22381 ctcggggtga cgctgggtaa cgcactgcat ttgtcacctg aagcgtcatt gtcgctgggt
    22441 gtatggtttg cgcgtattac cggactttcg atgttcctcg cttataccgg ggcgttcttt
    22501 acgctttgct attcaccgct gaaagccatc atccagggga cgccgaaagc gttgtggccg
    22561 gaaccgatga cgcgcctgaa tgcgatgggg atgccttcta tcgccatgtg gatgcagtgc
    22621 gggttggtta ctgtcttcat cctgctggtt tcgtttggtg gcggtaccgc atcggcgttc
    22681 tttaacaagc tgacgctgat ggcgaacgtg tctatgacgc ttccttacct gttcctcgcg
    22741 ctggctttcc cattctttaa agcacgtcag gatctcgaca gaccatttgt gattttcaaa
    22801 acgcgtatgt cggcaatgat tgcgacggtg gttgtcgtac tggtggtgac atttgcgaac
    22861 gtcttcacca tcattcaacc tgtggttgaa gccggagact gggacagcac attgtggatg
    22921 attggcggcc ctgtcttctt ctcgctgtta gcgatggcga tttaccagaa ctattgcagt
    22981 cgcatggcga ataaacctga gttagctctc gactgatcat atgaaatcct gcaactcatc
    23041 gtctctccgg cgatgagttg tcaggaagta atgcgtatgg atgatactct ccagggccgg
    23101 tgataactga agatgatgtt aagctgctaa atgagccacg ttgacatcat ttggcagctt
    23161 catgcattac aaacttcccg cctgacgacc tttgcccgca gaagttaatg ttctccccgt
    23221 ttgcttaccg ttgaggcttt ccagacgcat ctggaagggc gggaatggca tatcgatacc
    23281 gtgggcgtgg aagcctgcca gaatcagctg gtggatctca tggcgtatcg gcatacggtg
    23341 acccatctca gcggcgtaaa tacgcagctc gaaaatctga atcccctgtt gcagatccac
    23401 caggaagact tccggtgccg ggttgtcgat caccagcgag cagcgacgcg ctgcggtgag
    23461 caggatttcc gtcacttctt cgctattggc atcggcaggg gccggtatcg tcaacaccac
    23521 gcgcgtgacc gagtcagaga gcgaccagtt gataaactgc tcggtaataa acgccttgtt
    23581 cggcacgatt atctctttac ggtcccagtc gctgatggtg gtggcgcggg tgttaatttt
    23641 cgtcacgcta ccggtaagat cgcggatcgt taccgtatcg ccaatacgaa tcggtttttc
    23701 gaacaggata atcaggccag agataaagtt ggcgaaaatc tcctgcaaac caaaaccgag
    23761 accaacaccg agcgcggcaa ccagccactg caatttcgac cactcaatac caatcatcga
    23821 gaagccgacc agcccgccaa tcagcatcag cagatatttg gtgatggtgg tgatggcgta
    23881 gcccgtaccc ggcgttaaat ccaggtgctg caaaatcgcc agttccagca gcgcgggcag
    23941 gttgcgcacc agctgcgtgg tgatgataaa caccagaatg gcaatcagca ccgcaccgag
    24001 ggtaattggc tccagacttt ctacgccctg taccgtggag gtgacatccc acagcgaaat
    24061 attttcgagg aagccgaaag cggaatggat ttctgaccac agcacaatga cagaaagcag
    24121 ggcgatcaac atcaaaattg agcgcaccag ccgcaaggat tgcgcactga tggcatcgag
    24181 atcgacttcg ctttcatcga cttcaattgc cccttccggg ctactgtgat gatgcgcttc
    24241 ctcttcgcca cgcgcgcgtt gcgctaacat ctctgcccgg cgatgcttcg cccggtcaaa
    24301 cgccagcctg cgacgctgga tcagcatcca gcggcggata acgtgataaa ccaccagtag
    24361 caggaaccag atggcaaccg aggtttcaag cctcgctaac agtgcctgcg ccgttgccag
    24421 ataacccacc gctgacgcca gaatggcaac cagtggtgcg ccaatcatca tgttccacag
    24481 catatggttg gtaatgttgt cgccgctgcc ctctttgttg aggtacagcg ggatcccggc
    24541 ctttttcagg ctgagggtga ccaccgccag cgcaccgcaa atgaggataa agcaaagccg
    24601 tcccagcgaa ccggagaatt cacggtcgtc gaggttatcg aacatcatca gcgccataat
    24661 cagcggcaca ataagcccga tgctcatcag gtagtagcgc atcccacggg aaacacgttc
    24721 gcgcggccag ccaaaatgag cgataaacaa gccgttcggg cgggcaaagg tggcgcaaat
    24781 catcactacc cacagcagcg gcacggtggc cgttacgcca tcgccaatcg cgaccgccag
    24841 cggataaggc cacgcctcgc gcaagccgta acccagcgtc atccacagca ccggcagcgg
    24901 tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc agccagaagt gatcctgagt
    24961 cactttgccg actttcgccg ccgaacgttc aagaaaacgg gtgaaatagc ggcgcgagta
    25021 aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc aaaatcgtct ctttgctggt
    25081 cagcatcatc acactggctt tgcccaactg actgaaggtg tccagcgaaa tgagacgacg
    25141 cagatcctgg gcgatttcca gcggccaggc gatggtcatc gggcgcacgt cagaggtcca
    25201 gaacagatag cggtgcgttg cttcgttcac ctctttcagc gcatcctcca gttgcccgtt
    25261 ggagactttc agcttggtca gttccagtag tagcgtgtcg ccaccctgca acaatgagtt
    25321 cagcaactca cgctgagtgc gcagttgtgc ttccagaata cggttttgct cggcggtcag
    25381 cggctgaccg tcggcctgat gaatttgccg tagcagcggc tgtttattaa gcagatcctc
    25441 ataacgtaac cgttgcacac gcaactgcgc catttcggta tcaagctgtt gtggtttcgg
    25501 catttccggc agccgtgcca cctgcgcccg caacgcttcg ccgagcaggt tggacgatcc
    25561 cagccattgc gactgttcac gcagcgtatt cagcgcctgc cgaacctgta acgtctggct
    25621 ggtggcctgg cgctgttgcg aggcaacgag atccatccgc tgcgcctgtt gattcagtgc
    25681 cgccgatagt tcgcggttaa ttttgaattg cgcgacgatg tctttcggca aatcggcgct
    25741 gttttctgcc agcaattcgg tactttccag cgcccgctcc gcctcaagct gacgttggct
    25801 gttcaactga ttacgtaagg cctgcaaata cgcatccagt tgctggctct ctttttccgc
    25861 cagctctgag cgtaagcgcg ctaattcctg acggttattg gcagacagct gcgccagttc
    25921 cagttcatca acgagcgctt tcaggcgtgc ggagtcagac tgcaacgcga aattttgtgc
    25981 ctgattgagc ggggtattgc cagtaagcgt tcccaggcgg cgctcgatct catttaactg
    26041 acggcgggcg tcggtttgct gttgcggcag ttgattcagc gaatcggcaa tctcgcgggc
    26101 gcgctcctgc tcttgctggg cctgacggct tttatccagc aactggctgc tgacctggag
    26161 aatttcctga ttcagcgcgt cggtagacat tcccggcgac acgctgcgcg gctcgtcacg
    26221 catgttgttt aattgtgcgc gcagagtagc ggagagtttc ggataattat cgataacttg
    26281 ctgatattgt ttgatgcgct caagggaacc ttttcgttcc tcaagcgcat ttaaggcaga
    26341 ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc gcttttgcct gctccagttc
    26401 ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac gccccccaac tgaggcacca
    26461 ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt cctttgatgg attagacctg
    26521 gtctttttta tcgtcaacca atgggctggc gtcgtgttct gcttcgatct cttcagcagg
    26581 aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg gtagatactg ccagcggctg
    26641 gccaattttc gtgacagaca ggctttccag ttgctcaacc agattcactt tacccggtgc
    26701 aaacaggttg ataacggtag agccgagttt aaagcggccc atttcctggc ctttcagcag
    26761 tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag cgcttgatga taccttcgcg
    26821 cggcggcgta atggtgcccg cccagaccgt ctcaatgctg cctacaatcg tcgccccgac
    26881 cagaatctgc gccatcgggc caaattcggt atcgaacagg caaatcacgc gttcgttacg
    26941 ggcaaacaga ttcggcacgt tctgagcggt gagatggtta acggagaaga gatcgcccgg
    27001 cacgtagatc atctcacgca gaataccgtt gcacggcatg tgtacgcgat ggtagtcacg
    27061 cggggagagg taagtggtca caaacgtacc gttgcggaac aggtccgcca tcagatagtt
    27121 gcctgccagc agggcttcga ggctgtagtt gtggcctttg gcttgcagga ttttattttc
    27181 ttcgatttta cccaactggc tgataacgcc atcggcaggc atgaccagga cattcggatc
    27241 ggtatcgatt gggcgtactt cgtcacgcag cggacggaca aagaattcgt taaaggtgcg
    27301 gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg accttgtagt atttaacgaa
    27361 cagatcgata accagttttg tcagccatcc tgcccgcttg cttgcgcccc aacccgccag
    27421 gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa agtttaaatg aatttaacaa
    27481 ggtagcctcc aggccattgt tttgtcgttc ctgatccggc caacatgccg gatcctgaaa
    27541 aaaaggggac gattctaacg acggttagct taattgtcag tcatccgtat cagaaaagtt
    27601 tttacgcgtt tttacctgcg ccatgctttc cagaatacgg tgatagtttt cgaaacgggt
    27661 ttccgcgatt ttcccttcct caaccgcttc ccggatagcg cagcccggat cggtatcgtg
    27721 tttgcaatcg cgatatttac acagacctaa atagtcatgg aattcgacaa agccctgagt
    27781 gatttgttcc ggctccaggt gccagaggcc gaactcacgc acaccagggg aatcaatcac
    27841 atcaccgccg tgcgggaagt gatacagccg agcggcggtc gtggtgtgct gaccgagccc
    27901 cgagttgtca gagacatcgt tggtcaggat ctctttttgc agccccagca acgcattcag
    27961 caggctggat ttgccgacgc cagactgccc ggcaaaaatg ctgatgcgcc cggtcaacgc
    28021 ctcttccagc ggttttagcc catcctgagt atggctggaa accatcaata cgcgataacc
    28081 gatattgcgg tagatatcca tctgctcgtt gacgaacgcc atgccttcgt cgtccagcag
    28141 gtctatcttg ttgagcacaa taatcggctc aatctgcaag gtttcgcagg ccaccaggta
    28201 acggtcgata atattgagcg acagctccgg caaaatggcg gagacaatga caatctggtc
    28261 gatgttggcg gcaataggtt ttacgccgtc gtagaaatcc ggacgcgtca acaccgaggt
    28321 acgctcatgc accgcttcca cgatcccttt gacatttacg ccttccgccg ccggtttacc
    28381 cggacgccag actacgcggt cgccggttac cagcgaacgg atggtacggc gaatattgca
    28441 gcggtgaacg tcgccatcgg cggattccac atcagcgtgc ataccaaagc ggctgatgac
    28501 gataccttca tcaggctcgc caaacagatt gtcgtcgtag tcgggcttct ccttagacgt
    28561 tttaagacga cgctggtgat tggcgttcac gcggcgctgc tggcctttgg agagtttatt
    28621 tttactcaat cgtacagact cctggtcgcc cctgatgggc aaaacatcta tgatacacgc
    28681 aattgtggat caatatagtc actgtgaatg ggtggaaaat agcatgagtg ccaatgaaaa
    28741 caacctgatt tggatcgatc ttgagatgac cggtctggat cccgagcgcg atcgcattat
    28801 tgagattgcc acgctggtga ccgatgccaa cctgaatatt ctggcagaag ggccgaccat
    28861 tgcagtacac cagtctgatg aacagctggc gctgatggat gactggaacg tgcgcaccca
    28921 taccgccagc gggctggtag agcgcgtgaa agcgagcacg atgggcgatc gggaagctga
    28981 actggcaacg ctcgaatttt taaaacagtg ggtgcctgcg ggaaaatcgc cgatttgcgg
    29041 taacagcatc ggtcaggacc gtcgtttcct gtttaaatac atgccggagc tggaagccta
    29101 cttccactac cgttatctcg atgtcagcac cctgaaagag ctggcgcgcc gctggaagcc
    29161 ggaaattctg gatggtttta ccaagcaggg gacgcatcag gcgatggatg atatccgtga
    29221 atcggtggcg gagctggctt actaccgcga gcattttatc aagctgtaaa atttttcctg
    29281 gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt tgacgctaaa ttgaacgtct
    29341 tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa acttttttgg ggggttgcag
    29401 agggaaagat ttctcgtata atgcgcctcc cgtaacgacg cagaaatgcg aaaattacga
    29461 aagcaaaatt aagtagtacg cgggaatagc tcagttggta gagcacgacc ttgccaaggt
    29521 cgggg
//
LOCUS       DAZFYC010000056        29361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFYC010000056 DAZFYC010000000
VERSION     DAZFYC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.056"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            306..494
                     /gene="dicB"
                     /locus_tag="ACHKW5_002700"
     CDS             306..494
                     /gene="dicB"
                     /locus_tag="ACHKW5_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7512069.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            491..679
                     /locus_tag="ACHKW5_002701"
     CDS             491..679
                     /locus_tag="ACHKW5_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7512070.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            772..3447
                     /gene="recE"
                     /locus_tag="ACHKW5_002702"
     CDS             772..3447
                     /gene="recE"
                     /locus_tag="ACHKW5_002702"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO7512071.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     SSAQDKPASTDNTNVNGEDMTEIEENMLLQISGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARAEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGLDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLIETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKHDGEEKPQPSCAMADKQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKYQKLRAE
                     LHEARKTIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIETRDSVNQNQPESEQ
                     NNQKAEQNSPNAQKNEPETKQSEPVAQQEPEKVCAACGQSGGGNCPDCGAVMGDTTYQ
                     ETFNEENLDESQEKDPEEMEGPEHPHNENAGSDPHRDCSDETGEVADPVIAGDIEPGI
                     YYGISNENYHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLE
                     PEEFSNRFIVAPEFNRRTTAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQSVMAL
                     PLGQWLVESAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAY
                     YDYRYHVQDAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQE
                     YHRNLRTLADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            3440..4249
                     /gene="recT"
                     /locus_tag="ACHKW5_002703"
     CDS             3440..4249
                     /gene="recT"
                     /locus_tag="ACHKW5_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO7512072.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4306..4500
                     /gene="ralR"
                     /locus_tag="ACHKW5_002704"
     CDS             4306..4500
                     /gene="ralR"
                     /locus_tag="ACHKW5_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO7512073.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            4493..4681
                     /locus_tag="ACHKW5_002705"
     CDS             4493..4681
                     /locus_tag="ACHKW5_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO7512074.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            4788..5069
                     /locus_tag="ACHKW5_002706"
     CDS             4788..5069
                     /locus_tag="ACHKW5_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7512075.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            5035..6111
                     /locus_tag="ACHKW5_002707"
     CDS             5035..6111
                     /locus_tag="ACHKW5_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7512076.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(6504..6845)
                     /gene="yebY"
                     /locus_tag="ACHKW5_002708"
     CDS             complement(6504..6845)
                     /gene="yebY"
                     /locus_tag="ACHKW5_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO7512077.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(6858..7730)
                     /gene="copD"
                     /locus_tag="ACHKW5_002709"
     CDS             complement(6858..7730)
                     /gene="copD"
                     /locus_tag="ACHKW5_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO7512078.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(7734..8108)
                     /gene="yobA"
                     /locus_tag="ACHKW5_002710"
     CDS             complement(7734..8108)
                     /gene="yobA"
                     /locus_tag="ACHKW5_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO7512079.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            8247..8477
                     /gene="holE"
                     /locus_tag="ACHKW5_002711"
     CDS             8247..8477
                     /gene="holE"
                     /locus_tag="ACHKW5_002711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO7512080.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            8579..9235
                     /gene="yobB"
                     /locus_tag="ACHKW5_002712"
     CDS             8579..9235
                     /gene="yobB"
                     /locus_tag="ACHKW5_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO7512081.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            9259..9921
                     /gene="exoX"
                     /locus_tag="ACHKW5_002713"
     CDS             9259..9921
                     /gene="exoX"
                     /locus_tag="ACHKW5_002713"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO7512082.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(9918..11978)
                     /gene="ptrB"
                     /locus_tag="ACHKW5_002714"
     CDS             complement(9918..11978)
                     /gene="ptrB"
                     /locus_tag="ACHKW5_002714"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO7512083.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12187..12846)
                     /gene="yebE"
                     /locus_tag="ACHKW5_002715"
     CDS             complement(12187..12846)
                     /gene="yebE"
                     /locus_tag="ACHKW5_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7512084.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(13173..13529)
                     /gene="yebF"
                     /locus_tag="ACHKW5_002716"
     CDS             complement(13173..13529)
                     /gene="yebF"
                     /locus_tag="ACHKW5_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO7512085.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(13596..13886)
                     /gene="yebG"
                     /locus_tag="ACHKW5_002717"
     CDS             complement(13596..13886)
                     /gene="yebG"
                     /locus_tag="ACHKW5_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO7512086.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            14020..15198
                     /gene="purT"
                     /locus_tag="ACHKW5_002718"
     CDS             14020..15198
                     /gene="purT"
                     /locus_tag="ACHKW5_002718"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO7512087.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(15254..15895)
                     /gene="kdgA"
                     /locus_tag="ACHKW5_002719"
                     /gene_synonym="eda"
     CDS             complement(15254..15895)
                     /gene="kdgA"
                     /locus_tag="ACHKW5_002719"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO7512088.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(15932..17743)
                     /gene="edd"
                     /locus_tag="ACHKW5_002720"
     CDS             complement(15932..17743)
                     /gene="edd"
                     /locus_tag="ACHKW5_002720"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO7512089.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(17978..19453)
                     /gene="zwf"
                     /locus_tag="ACHKW5_002721"
     CDS             complement(17978..19453)
                     /gene="zwf"
                     /locus_tag="ACHKW5_002721"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO7512090.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     YNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            19791..20660
                     /gene="hexR"
                     /locus_tag="ACHKW5_002722"
                     /gene_synonym="yebK"
     CDS             19791..20660
                     /gene="hexR"
                     /locus_tag="ACHKW5_002722"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO7512091.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            20788..22230
                     /gene="pyk"
                     /locus_tag="ACHKW5_002723"
     CDS             20788..22230
                     /gene="pyk"
                     /locus_tag="ACHKW5_002723"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO7512092.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(22361..23332)
                     /gene="lpxM"
                     /locus_tag="ACHKW5_002724"
                     /gene_synonym="msbB"
     CDS             complement(22361..23332)
                     /gene="lpxM"
                     /locus_tag="ACHKW5_002724"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7512093.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(23452..24774)
                     /gene="mepM"
                     /locus_tag="ACHKW5_002725"
     CDS             complement(23452..24774)
                     /gene="mepM"
                     /locus_tag="ACHKW5_002725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO7512094.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(24790..25722)
                     /gene="znuA"
                     /locus_tag="ACHKW5_002726"
     CDS             complement(24790..25722)
                     /gene="znuA"
                     /locus_tag="ACHKW5_002726"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO7512095.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            25801..26556
                     /gene="znuC"
                     /locus_tag="ACHKW5_002727"
     CDS             25801..26556
                     /gene="znuC"
                     /locus_tag="ACHKW5_002727"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO7512096.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNRHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            26553..27338
                     /gene="znuB"
                     /locus_tag="ACHKW5_002728"
     CDS             26553..27338
                     /gene="znuB"
                     /locus_tag="ACHKW5_002728"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO7512097.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(27485..28495)
                     /gene="ruvB"
                     /locus_tag="ACHKW5_002729"
     CDS             complement(27485..28495)
                     /gene="ruvB"
                     /locus_tag="ACHKW5_002729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO7512098.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(28504..29115)
                     /gene="ruvA"
                     /locus_tag="ACHKW5_002730"
     CDS             complement(28504..29115)
                     /gene="ruvA"
                     /locus_tag="ACHKW5_002730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO7512099.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(29254..29319)
                     /gene="yobI"
                     /locus_tag="ACHKW5_002731"
     CDS             complement(29254..29319)
                     /gene="yobI"
                     /locus_tag="ACHKW5_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO7512100.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
BASE COUNT         7517 a         7353 c         7217 g         7274 t
ORIGIN      
        1 tgaggacacc gggttgtcag gttgaccata cgcttaagtg acaaccccgc tgcaacgccc
       61 tctgttatca attttctggt gacgtttggc ggtatcagtt ttactccgtg gctgctctgc
      121 cgcccttttt aaagtgaatt ttgtgatgcg gtgaatgcgg ctaagcgcac gcggaacagt
      181 taaaacaagc ggtcttttac gggcgtaacg ggaatgctct gtatccggcg ttaattgtta
      241 actggttaac gtcacctgga ggcaccaggc accgcatcac aaaattcatt gttgaggacg
      301 cgataatgga aacgttatta ccaaacgtta atacgtctga aggttgtttt gatattggtg
      361 ttctgctcag taaccgggag tttactgaag atgccattaa tatgaggaaa tatgagcctt
      421 atctgctcaa tgataattcc atactttccc gaattgctct tcttgaactt ggtattttcg
      481 gagaacgtca atgacttcag catttgcact gatgatgacg gtttttctta taacgggtga
      541 atcacagaat gtgattaccg gaatttatgc cagtaaagaa tcctgcctcc aggcaagaga
      601 cgagcaaaaa atttctggtg aatgcctccc ggtaaaaaaa gtatcgctgt acctgaataa
      661 cgaaacaccg gctggataac cctccagcca tattaacacc ataccaacgg attaaaaatg
      721 ccagcaatgg cagggatttg ttcaccctta aatctgtaat gaggtttatc aatgagcact
      781 gataaagaag aatttgcgct atattgcgaa gcaaaaaatg acaaagtcag aaaacgtctg
      841 ggaattaaag gtggttttta ctggactaca gcaaaaaaat tatctgttgc catctcccgc
      901 tgcattaccg caatggatga caacgattat gatgaagacg actttaaaaa acccgttcgc
      961 gtcaatttgc ccgttgttga tgaccttcca ccagagggcg tatttgatac ggaattctgc
     1021 aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc gccctcatcc
     1081 tctgcgcagg acaagccagc cagcactgac aataccaatg ttaatggcga agacatgacg
     1141 gagattgagg agaatatgct actccagatt tccggtcagg agctgcccat tcgctggctt
     1201 gctcaacacg gcagcgaaaa accggtaacg cacgtttcac gcgacgaact ccaggcatta
     1261 cacattgcac gggctgaaga actaccagct gttactgccc tggctgtttc ccacaaaacc
     1321 agcctgctcg acccgctgga aattcgcgat ctccacaaac tggtgcgtga tactgacaga
     1381 gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt cgaagcatac
     1441 ctggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa aggaaatcgt
     1501 gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa cctcaccgat
     1561 cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc cactggcgta
     1621 ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc taaacgtgtc
     1681 gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa attcatcacc
     1741 atgcctggcg ggctggatta ttcccgcgcc atcgtggttg cgtccgtaaa agaagcacca
     1801 attgggatcg aggttatccc tgcgcatgtc actgaatatc tgaacaaggt gctgattgaa
     1861 accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg ttcctctgcc
     1921 ccgatgccgc agcgtgtaac agaagaagga aaacacgatg gtgaagaaaa accgcaacca
     1981 tcttgcgcaa tggcagataa acaggcaacg gctgaaacag tggaaccgga tgcaactgaa
     2041 catcatcagg acacgcagcc gctggatgct cagtcacagg taaattctgt tgatgcgaaa
     2101 tatcagaaac tgcgggcaga actccatgaa gcccggaaaa ccattccgcc caaaaatcct
     2161 gtcgatgccg acaaattgct ggctgcctct cgcggagaat ttgttgaagg gattagcgac
     2221 ccgaatgatc cgaaatgggt taagggaatt gaaacccgag attctgtgaa ccagaaccag
     2281 cccgaatcgg aacaaaacaa ccagaaagcg gaacaaaaca gcccaaatgc acagaaaaac
     2341 gagccagaaa cgaaacaatc tgaaccagta gcgcaacagg aaccggaaaa agtctgtgcc
     2401 gcctgcggtc agagcggtgg cggcaactgc cctgattgtg gcgcggtgat gggcgacacc
     2461 acgtatcagg aaacctttaa tgaagaaaat ctggatgaat ctcaggaaaa agatccggag
     2521 gaaatggaag gccctgaaca tccgcacaat gagaatgctg gcagcgatcc gcatcgcgat
     2581 tgcagtgatg aaactggcga agtcgcagat cccgtaatcg caggagacat agagcctggt
     2641 atttattacg gaatttcgaa tgagaattac cacgcgggtc ccggtgtcag taagtctcag
     2701 ctcgacgaca ttgctgatac tccggctctg tatttgtggc gtaaaaatgc cccagtggac
     2761 accacaaaga caaaaacgct cgatttagga accgctttcc actgccgtgt acttgaaccg
     2821 gaagaattca gtaaccgctt tatcgtagca cctgaattta accgccgtac aaccgccgga
     2881 aaagaagagg agaaagcgtt tctgatggaa tgcgcaagca caggaaaaac ggttatcact
     2941 gccgaagaag gccggaaaat tgaactcatg tatcagagcg ttatggcttt gccgctgggt
     3001 caatggcttg ttgaaagcgc cggacacgct gaatcatcaa tttactggga agatcctgaa
     3061 acaggaattt tgtgtcggtg ccgtccggac aaaattattc ctgaatttca ctggatcatg
     3121 gacgtgaaaa ccacagcgga tattcaacga ttcaaaacgg cttattacga ctaccgctat
     3181 cacgttcagg atgcattcta cagtgacggt tatgaagcac agtttggcgt gctgccaact
     3241 ttcgtttttc tggttgccag cacaactgtt gaatgcggac gttacccggt tgagattttc
     3301 atgatgggcg aagaagcaaa actggcaggc cagcaggaat atcaccgcaa tctgaggacc
     3361 ctggctgact gcctgaatac cgatgaatgg ccagctatta aaacgttatc actgccccgc
     3421 tgggctaagg agtatgcaaa tgactaagca accacctatc gcaaaagccg atctgcaaaa
     3481 aacccaggga aaccgtgcac cagcagcagt aaaaaataac gacgtgatca gctttattaa
     3541 tcagccatca atgaaagagc aactggcagc agctctccca cgccatatga cggctgaacg
     3601 aatgatacgt atcgccacca cagaaattcg taaggttccg gcgctaggaa actgtgacac
     3661 catgagtttt gtcagtgcga tcgttcagtg ttcacagctc ggccttgagc caggtagcgc
     3721 cctcggccac gcatatttac tgccttttgg taataaaaac gaaaagagcg gtaaaaagaa
     3781 cgttcagcta atcattggtt atcgcggcat gattgatctg gctcgccgtt ctggtcaaat
     3841 cgccagcctg tcagcccgtg ttgtccgtga aggtgacgag tttagttttg aatttggcct
     3901 tgatgaaaag ttaatacacc gcccgggaga aaacgaagat gcaccagtga cccacgtcta
     3961 tgctgtcgca agactgaaag acggagggac tcagtttgaa gttatgacgc gcaaacagat
     4021 tgaactggtg cgcagccaga gtaaggctgg taataacggg ccatgggtaa ctcactggga
     4081 agaaatggca aaaaaaacgg ctattcgtcg cctgtttaaa tacctgcctg tctcaattga
     4141 aatccagcgt gcagtatcaa tggatgaaaa ggaaccactg acaatcgatc cggcagactc
     4201 ctctgtatta accggggaat acagtgtaat cgataattca gaagaataat tcagcctggc
     4261 ggtgtaatgc accgccaacg tgagacagtt tttatgacaa aaattatgag atatgacgat
     4321 gttaaaccat gtccgttttg tggttgtcca tctgttacgg tgaaagcaat ttcaggatat
     4381 taccgggcaa aatgcaacgg atgcgaatcc cgaactggct atggtggaag tgaaaaagaa
     4441 gcactcgaaa gatggaataa acgaactact ggaaataata atggaggtgt tcatgtataa
     4501 aataactgcc actattgaaa aagaaggtgg cactcctact aactggacaa gatattcaaa
     4561 atctaaatta acgaaatcag aatgcgaaaa aatgctctca gggaaaaaag aagcaggcgt
     4621 gtccagagag cagaaagtaa agctgataaa ttttaattgc gagaaacttc tgtcctcgtg
     4681 agttgcatta tatacaaatt agaacttcat agctgattat taaaaatcaa ccacacccgc
     4741 cagtattctg tatatttact ggcggtcata tcgtaagagg tatggcaatg aatcttgtga
     4801 cactcaaaac gtggggaaaa ctcagatatc cggataaccc accatcaata tcaacgctga
     4861 gacgatgggc aaggaatgga aacatttatc ctgcacctga actacacggg aggagttaca
     4921 gggtggttcc agaggctttc tatatcaacc cgaataaggt tgataccgat ataacacacc
     4981 atcagcctaa tgggcgacaa gggagagaca gtccgttaat ggagaagtta aaacatgcag
     5041 cggaaaaaat acgatcccaa tttgcctaaa aacttaacat atcgaaggag ggacaaagca
     5101 tattactggc gcaaccctct gacgaaagaa gaatttacac taggtaaaat ttcaagaaga
     5161 gatgcagtag cgcaggcaat tgaagcaaat cattatatat acaaaaacta ctctcctgct
     5221 gccttaattg aaaagcttaa agggttcgac tcatttacta tggcagactg gattgaacgt
     5281 tacaaaacga ttcttataag gagaaaagtg tccagaaata cttataaaat tcgggtaaat
     5341 caactggaga caataaaaga aaaattggga gagattttac tgacagaaat aaccactcgc
     5401 catattgccg agtttcttga tttgtggatt gaaggaggga aaaacacaat ggcaggatca
     5461 atgcgttctg tgttgtctga tatgtttcgc gaggccattg ttgaaggacg tatatctcaa
     5521 aatccagtaa cgccaacaag agcaccgaaa atagtagtta caagagaacg actgaaacta
     5581 aagacataca actgcatcag ggaggcagca gatcaacttc cggcatggtt cccattagct
     5641 atggatttag cccttgtaac aggacaacgt cgcgaagaca taacgaatat gcggttcagt
     5701 gatatttatg atgatcgtct ccacatcagg caaattaaga caggaatgat gattgctatc
     5761 cccctgtcac tcagccttcc tgtcgctggt ctacggcttg gtacagtagt tgaacggtgc
     5821 cgcctggtaa gccggggaga ttatctaatc agtgccggga ttagaaaaaa cagccctgac
     5881 ggcagcattc acccggacgg cctgacaaaa aaatttgtcg cagccagaaa attaacaggt
     5941 atccagttca gtgaaaaccc tccaactttt cacgagatca gaagtctggc tggacgattg
     6001 tacaaagaaa catgtggaga agaatttgct cagcgtctac ttggccacac atcggagaag
     6061 acaacaaaaa tgtatcttga tgagagagaa aaaacgtact tactgctctg attttaacgt
     6121 aaatggattg ttaaatgtat tttggttgtg atataaccaa aaaagaccgg aatacagaaa
     6181 ttcgagtaaa tttcggggaa tttcggggag acgtttgcaa ctaattgatt ttaaatacaa
     6241 ttaaaaaaag accgaatacg attcctgtat tcggtccagg gaaatggctc ttgggagaga
     6301 gccgtgcgct aaaagttggc attaatgcag gcttagttgc cttgcccttt aagaatagat
     6361 gacgacgcca ggttttccag tttgcgtgca aaatggtcaa taaaaagcgt ggtggtcatc
     6421 agctgaaatg ttaaaaaccg cccgttctgg tgaaagaact gaggcggttt ttttattgga
     6481 aatcaaaagg ctattttagg taattaacag agtttttcag ctcgttctat aaacggtgcc
     6541 agactcattt tttcgccggg attgttagga tcatcaatct gaatcaccga aatgggttgg
     6601 gctttagtct tcccactggc aacttccttt tgtgcgatat cgtttaaagg atactgcacg
     6661 agggtacttg ggttgatgac atacaaagca ttacccggtc ggcaagtcag catcacctct
     6721 tcgcgattaa acgcccattt gtctttaccc acttcaaaac ggctgacggt aatcacctgc
     6781 ggtgcagcca gcgccgctgc agaactggtg agtaacagaa acgccagaat actttttttc
     6841 atcattttcg taatccatca aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc
     6901 actaccgcac ctatcgccca ttcgagttta gtcatccact caaaccaggg tgccgcacga
     6961 tcttcatcct gcctcatgcg cggtacaaga acataacgat tagccagcgc aatgaccacc
     7021 ataatcatca ccaggatggc tttgagcaat aagagttggc cccagtaagt cgtaagcgtg
     7081 gggggaaatc cggtaatcaa caatgcatta agcacgccgc ttgccagtac gccgatcacc
     7141 gcaaaatgcc cgcaccagga aaaacgcatc agcgcctgaa tggcctggtg tcgccaacgg
     7201 cctttgatga gttgcataca ccagagcacc ggcagtaatc ccccaaacca cgccgccgca
     7261 caaatcaggt gaatcgcgtg attagtctga tggattttcg ccgttacccc ttcattcagt
     7321 gtcgcatgcc cgattcccgc cagcagaata aattgcgcgg tagtgagcat aaacagcaaa
     7381 cgtggcatat tccgcggttg cataagggcg acgatcaacg ttacgagggc gagaacaatt
     7441 tgccataacc agacgccacc aaactgcgtt tgcaaaacag cctgccaaat gtttggcgaa
     7501 aatacatctg cccatcccgt tcccatcaac ccgccctgaa cggcgagcat tgcagtggca
     7561 ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt cgccagcaag
     7621 cgacgaatcg ttaagggggc cagccaggca ccgtacattg cgaagccaaa gaccagcatc
     7681 agcgaggtga aatggataaa tcgcagcgcg atccaggtaa acgccagcat gatttatttc
     7741 acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca ccgaaacaac atgccagtca
     7801 acggtgtagg ttccgggttt cagcgaatcg gccagtggaa caatcagttg cttttggtcc
     7861 tgctcatttc gcttcgccgg taatgtttta atattttcgt tttttggccc cgtgattttt
     7921 gcaccactga atcctgtttc aacaccttcc gagaagttta aagtgattgc ctgcggtgca
     7981 gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg
     8041 gaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt
     8101 gaagtcatat tatctattcc tttttgtaat aactttttta cagagcataa ccttgtctaa
     8161 tgtctgagtc gaggatcatc aattccggct tgccatcctg gctcactctt agtaactttt
     8221 gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct ggctaaactg gatcaaacag
     8281 aaatggataa agtgaatgtc gatttggcgg cggccggggt ggcatttaaa gaacgctaca
     8341 atatgccggt gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg cgcagctggt
     8401 ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg
     8461 agcccaaact taaataaaac ttatacagag ttacactttc ttacataacg cctgctaaat
     8521 tatgagtatt ttctaaaccg cacccataat ttgcagtcat tttgaaaagg aagtcattat
     8581 gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca
     8641 ggtagctcat catctggagt ttgtgagggc cgccgccagg caacaatgtc agctgttagt
     8701 ttttccatcg ctttctttac tggggtgtga ttattcgcga cgtgcccttc ctgccccacc
     8761 cgatctctca ctgttagacc cgctttgtta tgccgcaacg acctggcgaa tgaccatcat
     8821 tgccggcctt cccgttgaat ataacgatcg ctttattcgt ggaattgcgg tgttcgcacc
     8881 gtggcgaaaa acgcctggga tctaccatca gagtcatggt gcatgtctgg gccgtcgttc
     8941 cagaaccatc acggtggtcg atgaacaacc gcaaggtatg gatatggacc cgacctgttc
     9001 actgtttact acagggcaat gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt
     9061 acaatttttt tcacatcagt actcgattgc cgtgctgatg gcaaatgccc gtggtaacag
     9121 tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt
     9181 agtcggtcag cgttcatcac agggttggca aggcgatatc attccattac gctaggcttt
     9241 ttcggcctgg agcatgccat gttgcgcatt atcgatacag aaacctgcgg tttgcaggga
     9301 gggatcgttg agattgcctc tgttgatgtc attgacggaa aaatcgtcaa ccccatgagc
     9361 cacctggtgc gccccgatcg tcctattagt ccacaagcga tggcgattca tcgcatcacc
     9421 gaagccatgg tcgccgataa accgtggatt gaagatgtga tcccacacta ttacggtagt
     9481 gaatggtatg tcgcgcataa cgccagcttt gaccgccgcg tactgcctga gatgcccggt
     9541 gagtggattt gcactatgaa actggcccgt cgtttgtggc ctgggatcaa gtacagcaat
     9601 atggcgttat ataaaacacg caagctcaat gtacagacgc cgccgggcct gcatcatcac
     9661 cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta tcgatattat gaacacctcc
     9721 ggctggacgg cagaacagat ggccgatatc accggacgtc cgtcgttgat gacgaccttc
     9781 acctttggca aataccgtgg caaagcggtt tccgacgttg ccgaacgcga tccgggctat
     9841 ctgcgctggt tatttaataa cctggacagc atgagcccgg agctgcgttt aacactgaaa
     9901 cattatctgg aaaatactta gtccgcaggc gtagcgggta atgttccctg cgccagcgcg
     9961 accagaaaag catattccat cgctacgcct tcgtacgatt taaagcgacc agatttaccg
    10021 ccatggcctg agtccatgtc ggtacagagc aataaaagat ggtcatcggt tttcagctcg
    10081 cgcaatttag cgacccattt tgccggttcc caatattgca cctgagaatc gtgcaaaccg
    10141 gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt tgtcatatgg gctgtagctt
    10201 ttcatgtact cgtaatattg cggatcctgc ggattccccc actcttcaaa ctcaccagtg
    10261 gtaagaggaa ttgattcatc aagcatcgtt gttacaacat caacaaacgg tacctgggcg
    10321 ataacgccgt ggaataattc cgggcgttga ttaattgcaa cgcccatcaa catgcccccc
    10381 gcactcccgc ccatcgcata acaaagcgaa ggagagccat agcccagttt taacaatgca
    10441 tcgcaggcat caagataatc attaaacgta tttttcttct tcagaaattt tccgtcttcg
    10501 taccattgtt gccccagctc accaccgccg cgaacatgga caatggcgta gacaaagcca
    10561 cgatctaaca aactcaagcg gctaaaactg aaatcggcat caatacttgc gccgtaagaa
    10621 ccatagccat acaccagcaa cgggttgtgt cctttgcgaa aatgtttgcg atggtagacc
    10681 aacgaaaccg gaacttcgac gccatcacgg gcgactatcc acaggtgttc actgcggtaa
    10741 ttcgccgcat caaaaccggg cacttctgtt tgttttaata cacgacgctc accggtatcc
    10801 atatccagtt caaacaaagt atctggtgtg gtcatggaag aataaccata acgcaatcgc
    10861 gcggtttcag gttctggatt gtaggcaatc caggtcacat aggccggatc atcaaaggca
    10921 ataccaatga cttcccgggt cttgcggtta atttggcgca aactggttaa cccgcgctga
    10981 cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc cttccagcat aatgttatcg
    11041 cgtggcggaa ttaactcttc ccactgttgc tcatcacgca ggcgggtacg gtataagcca
    11101 aagtttttgc catggcggtt ggaacgcaga taaaaccgat gctggtagtg atcaaggctg
    11161 tattcgtgat ctttgcggcg cggcagaaaa acaaacggct cggcatcggc catttccgcg
    11221 tccagcaggc gaacttcact ggtggtggcg ctggccaaat gaatgactac atagtgcttc
    11281 gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt tttcttcgta gatcagttta
    11341 tcttgcgatg ctggcgtacc gatggcgtga cgccagacct gataaggcag cagcgtcacc
    11401 ggatgcttgc gaacatagta gaaagtccag gagtcatttg cccagacaaa gctgggttca
    11461 acgttatcca gcagttccgg gtaccagtta ccagtttcca gattacgaaa acgaatgccg
    11521 tactggcgtc gggaaagaaa atcttctgcc agcgccataa tggtgttatc gggcgtaatc
    11581 gccattccgc ccatcgaata aaactcacta tgagctgcgc gcttattggc atcgagcaat
    11641 gtttcccact catcccactc ttcactgaat gccgattgac gctggtagat agcatattca
    11701 cagcctggtt cataaatatg ccgatagcgg tagccatttt tgatgtaggg cgcagaaact
    11761 tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa ggcttgttgt
    11821 gaggccatca cccgatgacc gtaactattt tcttgttgca ggtagtccag gacttctggc
    11881 tgagaacgcg tatcgtcccg cagccagtag taattatcga tgcgcgtatc gccatgaagc
    11941 gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt tattgttctt tctggttgaa
    12001 acatcttata agggtggcaa aactcaccgg ggatgcaagc gaaacagggg agtcattgct
    12061 tagatgatga caggtaatgg cgcggatatc gaatgttatg cagacagaga aaatcagcct
    12121 gttcaaatgg ctgtgcgatt ctggatagcc cgaaatagtc aacttcaggc tatccagaga
    12181 gcggaattat tccgccaaag tgcgtttttg ctgttcgaga tcgcgttcaa tgccgtcacg
    12241 aacatcctgg gggattttca gcgcgtcacc cagtgcattc aggtaactgc gttccataaa
    12301 atggtcaata tcaatagccg cgcaactcag gaaatagatt tccagcgcct cttcttcatt
    12361 gcggaccccg gtagccaggc gttgtggatc cagcggttgt tcgattgcct gctcaatgag
    12421 tacacgcccc tgctcttcca cgccggcttc acgcaattgc tggtcgattg ccgcacgttc
    12481 tttggcatca atatgaccat cacttttagc ggcaaagacc agcgcaagga tcaaacgttc
    12541 tgtacgctca tccagcggcg tactttgcgc gccaaactgc ggttcgtcct gatgcgccgc
    12601 gcgaatttta tctttgtatt tattccacag caccgtaccc gctaccgctc cgccgccaac
    12661 cagtaacgcg ttggtgccat atttcgtaag aagtttacgt gctgatttat ttgcgaccag
    12721 cagcccagcc agtccgccta atgcgcctgg cactaacagt ttgcccaatc cctgatccgc
    12781 agacgaggag gtagaagaac tgctttgccc aagcagggat tgcagttgat ttaaccagtt
    12841 agccatattt gctcctcaat aaccattttt agtgcctgta gcatagcgga tgaggatgtc
    12901 agaaaatgta tatcgagaca aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa
    12961 agaaattcgc gccaggtaag gtatttactg tatatcaggt gataagggta ttttaggtga
    13021 aagtagtttg tcgttttgag tggcattgtt tgatgctgat tgcccgatgc gacgctgttg
    13081 cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat
    13141 ccgccaacgg tcaatcgaat tatcagaagc cattaacgcc gctgatattc cgccattccc
    13201 gctttgcagt ccacgctgac ctggtaatga atatcggcac ttttaccacg cacggttagc
    13261 ggtactgacc atttatcatc tttaccctga atgtcctgca aactgaccca agccacggga
    13321 tcggcctgac cgacaatttt ttgatcatct gcccaacgcg ccacgcgatt ttgttgataa
    13381 tcacgtttta cgctcgcggc aattccggca gcatccagac cttcacactt tgggaaagtg
    13441 accgacttgc tggtttcatt attggcagcg aaaactgatg cgcaggcaga aaccaacaac
    13501 agccctaaaa acgcccctct ttttttcatg tttttctcca tagcacaatg attcaggaga
    13561 aagcatggta caaattgtca ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc
    13621 tcttcggatt cagcaccgac gacctgtggg gacggtaatt tgccggtttt cagaatggtg
    13681 ctcagcacat ctttttgttc tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc
    13741 atttgcactg gagaatttgt cagccaggtg tcaagcagat ccgccgtatc aagcatttta
    13801 tcataagcat cggcttcctt tttgctggta aacgacattt tctcttcgcc ctcacgaatg
    13861 actacgtatt taacttcaac cgccatttgc agcctctcat aataactgtg attttataca
    13921 gtatatttct tttcggttga gaaatcaaca tcagcaataa agacacacgc aaacgttttc
    13981 gtttatactg cgcgcggaat taatcagggg atattcgtta tgacgttatt aggcactgcg
    14041 ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg gtgaactggg taaagaagtg
    14101 gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg tcgatcgcta ttccgacgca
    14161 ccagccatgc atgtcgcgca tcgctcccat gtcattaata tgcttgatgg tgatgcatta
    14221 cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc cggagatcga agctattgcc
    14281 accgatatgc tgatccaact tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca
    14341 acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg
    14401 cccacttcca cttatcgttt tgccgatagc gaaagccttt tccgcgaggc ggttgccgcc
    14461 attggctatc cctgcattgt aaaaccggtg atgagctctt ccggcaaggg gcagacgttt
    14521 attcgctctg cagagcaact tgctcaggca tgggaatacg ctcagcaagg cggtcgcgcc
    14581 ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg acttcgaaat taccctgcta
    14641 accgtcagcg cggtggatgg cgtccatttc tgtgcaccag taggtcatcg ccaggaagat
    14701 ggcgactacc gtgaatcctg gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg
    14761 caggagattg cccgtaaagt ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag
    14821 ctgtttgtct gtggtgatga ggtgattttc agtgaggtct cccctcgtcc acatgatacc
    14881 gggatggtga cgttaatttc tcaagatctc tcagagtttg ccctgcatgt acgtgccttc
    14941 ctcggacttc cggttggcgg gatccgtcag tatggtcctg cagcttctgc cgttattctg
    15001 ccacaactga ccagtcagaa tgtcacgttt gataatgtgc agaatgccgt aggcgcagat
    15061 ttgcagattc gtttatttgg taagccggaa attgatggca gccgtcgtct gggggtggca
    15121 ctggctactg cagagagtgt tgttgacgcc attgaacgcg cgaagcacgc cgccggacag
    15181 gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa aaatgcccga tcctcgatcg
    15241 ggcattttga cttttacagc ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc
    15301 gatcgtaatc gcccgcttcc agcgcatctg ccggaaccag ccaggaacca ccgatgcaca
    15361 gcacgctttt cagcgccagg tagtcacggt agttagccgg agaaatacca cccgtcgggc
    15421 agaaacggac ctgggagaac ggacccgcga tcgcctgcag ggctttcacg ccgccgttag
    15481 cttcagccgg gaagaatttg aactctttca aaccgtagtc catacccagc atcagttcgg
    15541 aaacagtgct gatccccgga attagaggaa tagtcccttc ggtagcagct ttcagcagcg
    15601 gctcggtcag acccgggcta attgcgaact gtgcacccgc ttcagtgact tctgccagct
    15661 gctgtggatt cagcaccgta ccggcaccca caatcgcttc aggcacttct ttggcgatag
    15721 cacggatagc gtcaactgca cactcggtac gcagagtcac ttccagaacg cgcaccccac
    15781 cagcaaccaa cgcttttgcc atcggcactg cgtgttccag tttttttacc acgataaccg
    15841 gtacaaccgg gccggtggtc aggattgatt ctgcacttgt tttccagttt ttcatcagag
    15901 ttttctctcg cctgattaca aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt
    15961 cggcaccgga cagtttttca cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg
    16021 acgcgctcag gtcaggaatg tgcggttcgc gagcagccag ttccgcttcg tctaccagca
    16081 gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc cccgtcgcgc acttttgcca
    16141 gcagcccgcc atcgtaggct tctggtgtta cgtggatagc tgacggcact ttacctgaag
    16201 cgccggagag tcgtccatcg gtaactaacg caattttgaa acaccggtcc aataatacac
    16261 caagtggcgg catgagttta tgtaattctg gcattccgtt cgcttttggc ccctgatgac
    16321 ggacaacaac gacacaatcg cggtccagca aacccgcttc aaaggccggc ataacgtcat
    16381 gctggctttc aaaaacaacc gctggcgctt caatcacctg gttctcaacc ggcacggcag
    16441 aggttttcat aaccgcacgg cccaggttac cgcttaacac ttttgtccca ccatgatgag
    16501 agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag tgatttttcc gccccttccc
    16561 gccagtccag ttcaccatta ttcagccatg gttcaagggt ataacgagac agaccaaagc
    16621 ctgccaccgt attgacatct tcatgcagca ggcctgcttt gagcagttca cgcaccagaa
    16681 ccggtacgcc acctgccgcc tggaagtggt taatatcggc cggaccgttc gggtagagac
    16741 gtgccatcag cggtacaaca tcagaaaggt cagagaagtc atcccagtta atctgaatac
    16801 cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt agtggaacca ccggtcgcca
    16861 gcagtgcaac gataccgttc accaccactt tctcatcgat catcttaccg atcggcatcc
    16921 attcattacc attaccggtc atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat
    16981 cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg caactgcatc cccataaatt
    17041 ccaccaccat ctggttggtg ttggcagtac cgtagaaagt acatgttccc ggcgcatggt
    17101 aagacgcggc ttctgactcc agtaaggcca tgcggtccac tttaccttcg gcataaagct
    17161 ggcgaatacg cactttttct ttatttggca aaccgcttgc catcggtcca gacggcacaa
    17221 acaccgcagg caaatgacca aacgacaggg ctgccatcgt cagacccggg acaatcttgt
    17281 cgcacacacc gaggaacaga gcaccatcaa acatgttatg ggacagcccc accgccgcag
    17341 acatcgctat cacttcgcgg cttagcagcg acaattccat tccatcctgc ccctgggtga
    17401 caccatcaca catcgccgga acaccgcccg caacctgacc aaccgcattc gcttcatgca
    17461 gggctttacg aatgatttct ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt
    17521 tataggaggt gatgatggcg atattgttac gcaacatgct tttcaaagag gctttgtctt
    17581 ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa
    17641 cggtcgacgt tttcgcttgt tctatccggg cgagataagc agagcgagtc tcgcgcgaac
    17701 gttcaatgat tcgatttgtt acgcgtaaca attgtggatt cataaaggct cctgaaattg
    17761 agttgtcaga gcaggatgat tcacaacgcg tttcattcag aggatttatg agtgaaacgc
    17821 ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg
    17881 ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct acaatctgtg caagatcatg
    17941 ttaccggtaa aataaccata aaggataagc gcagatatta ctcaaactca ttccaggaac
    18001 gaccatcacg ggtaatcatc gccaccgagg caacgggtcc ccaggttccg gcctgatacg
    18061 gtttcggcgc atcattgtcc atcgcccacg cctcagtaat ggagtctacc catttccagg
    18121 cttcttccac ttcgtcgcga cgtacaaaca gtgcctgaat accacgcatg gtttccagca
    18181 gcaaacgttc ataggcatcc gccagatgcg tctgattaaa ggtttctgaa tagctcagat
    18241 ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct
    18301 ggatatccac gccttcatca ggttgcagac ggatagtcag tttattctgc ggcagatcct
    18361 gccacgactc tttaaacaga ttcagttcag gtgttttgaa ataaaccacg acttcagaac
    18421 atttggtcgg cagacgttta ccagtacgca ggtagaatgg cacaccggcc cagcgccagt
    18481 tatcaatgtc gacgcggatc gccacgaagg tttctgtatt gctgctcttg ttcgcgccct
    18541 cttcttccag atatcccggc acttttttgc cctgggcgaa gcccgcagta tattgcccgc
    18601 gtacggtttt ttcgcgtacg ttgtagcggt cgatgcggcg cagagacttc agtactttca
    18661 ctttttcatc gcggatgctg tctgcgctca ggtcagacgg cggagacatc gcaatcatgc
    18721 aaagaatttg cagcaggtgg ttctggatca tgtcgcgcat ctgaccggct ttatcaaaat
    18781 agccccagcg cccttcgatc cccacttctt ctgccacggt aatctcaaca tgatcaatgg
    18841 tgcgattgtc ccagttattc acaaacaggg agttagcaaa acgcagcgcc aacaggttca
    18901 gcaccgtttc tttaccaaga tagtggtcga tacggtaaac ctggcactcc tcgaagtatt
    18961 cgccaacctg atcattgatt tcctgcgagg tcgccagcga cgtccccagc ggtttctcca
    19021 tgactacgcg tgccggttta gcattcagtt ttgcctcgcc aagccctttg caaattgcgc
    19081 caaaagtgct gggcggcatg gcaaagtagt taatggtgat acgatttttt tgatccagca
    19141 tcgcgccgag acggctgaat gcagcagtgt cattgacatc gagattacaa aaatccagac
    19201 gtgcactcag ggtgtcccat aaaccttcat caatggtttc tttcatgaaa gtttcgagcg
    19261 cctcgcggac aactttggta tatgccgctt tatcccagtc agcacgccct acgccgataa
    19321 tccgggtgtc cgggttgagc tgaccggctt tttccagttg atacagggaa ggcagcaatt
    19381 tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt
    19441 gcgttaccgc catgtcattc tccttaagtt aactaacccg gtacttaagc cagggtatac
    19501 ttgtaatttt cttacggtgc actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct
    19561 tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc
    19621 atcgttttcc attgggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt
    19681 ttttatctgc ttttgtcatt aacacggcac acaggcgtaa tatctgacaa aactgcattt
    19741 cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc tctttacatc atgaatatgc
    19801 tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg
    19861 tcattctggc ttcgcccgat aacgcgatcc attcgagtat tgctgctatg gcactggaag
    19921 ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag catggacacg cgcggttttc
    19981 ctgattttaa acttcatctg gcacagagtc tggcgaatgg cactccctat gttaatcgca
    20041 atgtcaatga agatgacagc gttgaatcat acacagggaa aatatttgag tccgcaatgg
    20101 caacgcttga tcatgtccgt cattcactgg ataaatctgc catcaaccgc gccgtcgact
    20161 tgctcactca ggcaaaaaaa atcgcctttt tcggattagg ctcttcagcc gccgttgccc
    20221 acgatgcgat gaataagttc tttcgtttta atgttccggt ggtgtactcc gatgatatcg
    20281 tgctgcaacg catgagttgt atgaattgta gcgacggaga cgtggtggtg ctgatttctc
    20341 acactggaag aacaaaaaat ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcca
    20401 tggtgattgc cctcacctct gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta
    20461 ccctcgacgt accggaagat actgacattt atatgcccat ggtttctcga cttgcacagc
    20521 tgaccgtgat agatgtgctg gcgacaggat ttactttgcg acgcggtgca aaattcagag
    20581 ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg ttttgataag cagttactta
    20641 atttaagtga cgatcgctaa aaacgactgt cactgtccta atcttatacg acatccgaat
    20701 gagattaatt tatcgccatc gcggcgttat ttcattcgga tttcatgttc aagcaacacc
    20761 tggttgtttc agtcaacgga gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt
    20821 accacgttag gcccagcaac agatcgcgat aataaccttg aaaaagttat cgcggcaggt
    20881 gccaacgttg tacgtatgaa cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg
    20941 gataaagttc gtgagattgc cgcaaaactg gggcgtcatg tggctattct gggtgacctc
    21001 caggggccca aaatccgtgt atccaccttt aaagaaggca aagttttcct caatattggg
    21061 gataaattcc tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt
    21121 atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg acatcctgct gctggacgat
    21181 ggtcgcgtcc agttaaaagt actggaagtt cagggcatga aagtgttcac cgaagtcacc
    21241 gtcggtggtc ccctctccaa caataaaggt atcaacaaac ttggcggcgg tttgtcggct
    21301 gaagcgctga ccgaaaaaga caaagcagac attaagactg cggcgttgat tggcgtagat
    21361 tacctggctg tctccttccc acgctgtggc gaagatctga actatgcccg tcggctggca
    21421 cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc
    21481 agccaggatg caatggatga catcatcctc gcctctgacg tggtaatggt tgcacgtggc
    21541 gacctcggtg tggaaattgg cgacccggaa ctggtcggca ttcagaaagc gttgatccgt
    21601 cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc agatgatgga gtcaatgatt
    21661 actaacccga tgccgacgcg tgcagaagtc atggacgtag caaacgccgt tctggatggt
    21721 actgacgctg tgatgctgtc tgcagaaact gccgcagggc agtatccgtc agaaactgtt
    21781 gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa
    21841 caccgtctgg acgttcagtt cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac
    21901 gcagctaacc acctgaaagg cgttacggcg attattacca tgaccgaatc gggtcgtacc
    21961 gcgctgatga cctcccgtat cagctctggt ctaccaattt tcgccatgtc gcgccatgaa
    22021 cgtacgctga acctgactgc tctctatcgt ggcgttacgc cggtgcactt tgatagcgct
    22081 aatgacggcg tagcagctgc cagcgaagcg gttaatctgc tgcgcgataa aggttacttg
    22141 atgtctggtg acctggtgat tgtcacccag ggcgacgtga tgagtaccgt gggttctact
    22201 aataccacgc gtattttaac ggtagagtaa gtacgttgcc ggatgcggcg aaaacgccac
    22261 atccggccta cagttcaatg atagttcaac agatttcgaa tattctgaag caaacttgaa
    22321 cttatcatca ggcgaaggcc tctcctcgcg agaggctttt ttatttgatg ggataaagat
    22381 ctttgcgctt atacggctgg atttcgcccg gtttgcgagt tttcagcaat tttagtatcc
    22441 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
    22501 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
    22561 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
    22621 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tcaacaaatt
    22681 cgctgtgctc agggccatga tcctgatcgg gtaaataata tccccagtag ccctgacgta
    22741 ccgactggat gaatggttta ataccatcat ttctcgcatg cagacgaccg ccaaaacgac
    22801 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
    22861 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
    22921 gcaccagaaa gataactttc tcgttattac gccgcatctc ttcgatgatc tccagccctt
    22981 gccagtcaac gcgcggctga attttctccg gcccgcgtat tgccaactca gccatcattg
    23041 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
    23101 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
    23161 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
    23221 aagcgatacc cgccatcgct gctacgccca gccatgctcc ccagtagcgc gggtggcgaa
    23281 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
    23341 agtttcggat aaggcaaaaa tcaatctggt gatagtgtag cggcgcaact tgccccgcac
    23401 caaataaaaa aagccggtac tgacttcgta ccggctgcga atggatgtta attaatcaaa
    23461 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
    23521 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
    23581 tacttcatag tgcaaatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
    23641 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
    23701 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
    23761 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
    23821 atcaacacct ctgtgtggtg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
    23881 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
    23941 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
    24001 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
    24061 tcgagacatt aataccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
    24121 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
    24181 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
    24241 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
    24301 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
    24361 ttgttgaccg attttcaggt tacgcaattc tttgtcggca gcagccagtt gggtgatatc
    24421 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
    24481 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct gaggaatggc
    24541 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
    24601 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
    24661 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
    24721 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
    24781 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
    24841 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
    24901 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
    24961 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
    25021 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc caaactgttt
    25081 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
    25141 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
    25201 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
    25261 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
    25321 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
    25381 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctt ctggtaattt
    25441 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
    25501 gttctgtaag cgttttacat ccgatggacg cagtgaataa tcatgttctg aagcgccgtc
    25561 aggcagtaaa acctccgttt ctgttacacc atcagcaatg gcagaagcga tgaacccaac
    25621 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
    25681 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
    25741 aatgaatatg aggagtgtga tattataaca tttcatgact actgcaagac taaaattaac
    25801 atgacaagtc tggtttccct ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
    25861 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
    25921 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
    25981 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
    26041 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
    26101 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
    26161 ctctctggtg gcgaaacgca gcgtgtacta ttagcgcgag cattgttaaa tcgcccgcaa
    26221 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
    26281 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
    26341 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcgcca catttgttgt
    26401 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
    26461 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
    26521 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
    26581 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
    26641 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggcgtc gcgtttggtt
    26701 tgttgctgga cgtgaatcca ttctatgcgg taattgccgt tacgctgctc ctggcgggcg
    26761 gtctggtatg gctggagaag cgtccacagc tggcgattga cacgttatta gggattatgg
    26821 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaac attcgtgttg
    26881 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatcgcta
    26941 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
    27001 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
    27061 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gttggcgcgt
    27121 tgattattac ttcactgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
    27181 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
    27241 ccttttccgc gttttacgat acgccggcgg gtccgtcggt ggtgctatgt gcggcactgt
    27301 tatttattct cagtatgatg aaaaagcagg ccagctaatc tatcgctgaa cacatttgtc
    27361 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
    27421 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtacga gcgaccaatc
    27481 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
    27541 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
    27601 cctcaatggt ttcacgttct tcgccaatag ccgccgccag gttatccaga cctacaggcc
    27661 caccaaagaa tttatcgatt accgccagca acaatttgcg gtccatataa tcaaaacctt
    27721 cagcatcgac attcagcata tccagcgcct gagcagcgat atctgccgag atggtgccat
    27781 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atgcgcggcg
    27841 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
    27901 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
    27961 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgt gtggttgcac
    28021 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
    28081 tcatgatatc cagttggtag tcttccattg ccggatacaa cacctcttca acaactggcg
    28141 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
    28201 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
    28261 tttcattggc cacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
    28321 acaaatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
    28381 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
    28441 ctacatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
    28501 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagggcg
    28561 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
    28621 ggcaaccgct tcttgttcag catcgtcggt cgccgggctg gcaggagacg tgagcaccag
    28681 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
    28741 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
    28801 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
    28861 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgctt
    28921 attgttaaag ccgtacagca gctgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
    28981 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
    29041 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
    29101 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
    29161 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggcgt cccaacgatc
    29221 tgaaaaatgg cgcagtataa tttattctac aggttatatt ggaagcaaat atttgaatat
    29281 tacatattca gtgaagaaat gcgtaataaa aatatacatt gcgactcctg aaataataaa
    29341 ttttttatgc tattacgtat a
//
LOCUS       DAZFYC010000057        29127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFYC010000057 DAZFYC010000000
VERSION     DAZFYC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.057"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..1797)
                     /gene="glnS"
                     /locus_tag="ACHKW5_002732"
     CDS             complement(133..1797)
                     /gene="glnS"
                     /locus_tag="ACHKW5_002732"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO7512101.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2000..3946)
                     /gene="nagE"
                     /locus_tag="ACHKW5_002733"
     CDS             complement(2000..3946)
                     /gene="nagE"
                     /locus_tag="ACHKW5_002733"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO7512102.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDY
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4279..5079
                     /gene="nagB"
                     /locus_tag="ACHKW5_002734"
     CDS             4279..5079
                     /gene="nagB"
                     /locus_tag="ACHKW5_002734"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7512103.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKICSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5139..6287
                     /gene="nagA"
                     /locus_tag="ACHKW5_002735"
     CDS             5139..6287
                     /gene="nagA"
                     /locus_tag="ACHKW5_002735"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7512104.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6296..7516
                     /gene="nagC"
                     /locus_tag="ACHKW5_002736"
                     /gene_synonym="nagR"
     CDS             6296..7516
                     /gene="nagC"
                     /locus_tag="ACHKW5_002736"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO7512105.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7564..8316
                     /gene="nagD"
                     /locus_tag="ACHKW5_002737"
     CDS             7564..8316
                     /gene="nagD"
                     /locus_tag="ACHKW5_002737"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO7512106.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8573..10237
                     /gene="asnB"
                     /locus_tag="ACHKW5_002738"
     CDS             8573..10237
                     /gene="asnB"
                     /locus_tag="ACHKW5_002738"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO7512107.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10618..10694
                     /locus_tag="ACHKW5_002739"
     tRNA            10618..10694
                     /locus_tag="ACHKW5_002739"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10652..10654,aa:Met,seq:cat)
     gene            10703..10787
                     /locus_tag="ACHKW5_002740"
     tRNA            10703..10787
                     /locus_tag="ACHKW5_002740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10737..10739,aa:Leu,seq:tag)
     gene            10811..10885
                     /locus_tag="ACHKW5_002741"
     tRNA            10811..10885
                     /locus_tag="ACHKW5_002741"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10843..10845,aa:Gln,seq:ttg)
     gene            10920..10994
                     /locus_tag="ACHKW5_002742"
     tRNA            10920..10994
                     /locus_tag="ACHKW5_002742"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10952..10954,aa:Gln,seq:ttg)
     gene            11010..11086
                     /locus_tag="ACHKW5_002743"
     tRNA            11010..11086
                     /locus_tag="ACHKW5_002743"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11044..11046,aa:Met,seq:cat)
     gene            11135..11209
                     /locus_tag="ACHKW5_002744"
     tRNA            11135..11209
                     /locus_tag="ACHKW5_002744"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11167..11169,aa:Gln,seq:ctg)
     gene            11247..11321
                     /locus_tag="ACHKW5_002745"
     tRNA            11247..11321
                     /locus_tag="ACHKW5_002745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11279..11281,aa:Gln,seq:ctg)
     gene            complement(11475..12650)
                     /gene="ubiF"
                     /locus_tag="ACHKW5_002746"
     CDS             complement(11475..12650)
                     /gene="ubiF"
                     /locus_tag="ACHKW5_002746"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO7512108.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12796..14220
                     /gene="miaB"
                     /locus_tag="ACHKW5_002747"
     CDS             12796..14220
                     /gene="miaB"
                     /locus_tag="ACHKW5_002747"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO7512109.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14373..15413
                     /locus_tag="ACHKW5_002748"
     CDS             14373..15413
                     /locus_tag="ACHKW5_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO7512110.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15410..15877
                     /gene="ybeY"
                     /locus_tag="ACHKW5_002749"
     CDS             15410..15877
                     /gene="ybeY"
                     /locus_tag="ACHKW5_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO7512111.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15967..16845
                     /gene="corC"
                     /locus_tag="ACHKW5_002750"
     CDS             15967..16845
                     /gene="corC"
                     /locus_tag="ACHKW5_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO7512112.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16870..18408
                     /gene="lnt"
                     /locus_tag="ACHKW5_002751"
     CDS             16870..18408
                     /gene="lnt"
                     /locus_tag="ACHKW5_002751"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO7512113.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYKSADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18486..20363)
                     /locus_tag="ACHKW5_002752"
     CDS             complement(18486..20363)
                     /locus_tag="ACHKW5_002752"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO7512114.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20818..21726
                     /gene="gltI"
                     /locus_tag="ACHKW5_002753"
     CDS             20818..21726
                     /gene="gltI"
                     /locus_tag="ACHKW5_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO7512115.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21896..22636
                     /gene="gltJ"
                     /locus_tag="ACHKW5_002754"
     CDS             21896..22636
                     /gene="gltJ"
                     /locus_tag="ACHKW5_002754"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO7512116.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22636..23310
                     /gene="gltK"
                     /locus_tag="ACHKW5_002755"
     CDS             22636..23310
                     /gene="gltK"
                     /locus_tag="ACHKW5_002755"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO7512117.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSIKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23310..24035
                     /gene="gltL"
                     /locus_tag="ACHKW5_002756"
     CDS             23310..24035
                     /gene="gltL"
                     /locus_tag="ACHKW5_002756"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO7512118.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24153..25088
                     /gene="rihA"
                     /locus_tag="ACHKW5_002757"
     CDS             24153..25088
                     /gene="rihA"
                     /locus_tag="ACHKW5_002757"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO7512119.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRTIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25172..26842
                     /gene="hscC"
                     /locus_tag="ACHKW5_002758"
     CDS             25172..26842
                     /gene="hscC"
                     /locus_tag="ACHKW5_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO7512120.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26902..28353)
                     /gene="djlC"
                     /locus_tag="ACHKW5_002759"
     CDS             complement(26902..28353)
                     /gene="djlC"
                     /locus_tag="ACHKW5_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO7512121.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKSHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28350..29057)
                     /locus_tag="ACHKW5_002760"
     CDS             complement(28350..29057)
                     /locus_tag="ACHKW5_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7512122.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEDSLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         7159 a         7561 c         7358 g         7049 t
ORIGIN      
        1 gataaattgc gaagcggaat taattcgcga agaaaagcag aaaaaacgcc gcagagtgcg
       61 gcgtttggga ttgtttgcta tttcttgccg gatgcgacgt aaatgcctca cccggcctgg
      121 tagaacttaa aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt
      181 aaataccggt ttttccgctg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc
      241 aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt
      301 gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac cagggttcgg
      361 cacgctgaac agacggtcgt acagacggat ttcaaccggc agcgcatgtg ccgcgctcac
      421 ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc
      481 gtcataagta cagaagatgg tggtgatatt gccttcggca tctttctcca cgcgttcagc
      541 cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg
      601 cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atctcaccgc taaacggcac
      661 ctgacgactc cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc
      721 gccctgatag ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc
      781 attttcgttg agatcttcac ggatgcagga ttccagcgat gccatctcaa tggtgttgtc
      841 ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata gaagcagcag tgtaaccacg
      901 acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc
      961 ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga
     1021 gaattcgtac tggcgcgggt gaacaggaat ggtgatgttg tccagcaccc agtcgtacag
     1081 gcgacggttg tcctgaaact caagcgtaca cagagagtgc gtaatacctt ccagggcatc
     1141 gctgatgcag tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg
     1201 gtgttcagca aatttaatac ggtacagcac cggatcgcgc atcacgataa acggtgaagc
     1261 catgtcgatt ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt
     1321 ttcgaacagc gccaggttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg
     1381 ttgcgtcagg gtgccgcggt attcgcggat ctgttccggc gtcagttcat caacgtacgc
     1441 caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga
     1501 gtaacggacg ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgactc
     1561 aacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc
     1621 tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag
     1681 atagccattc ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac cactggccag
     1741 atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt
     1801 ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg
     1861 acaactgtta gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa
     1921 aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctcaagcgtc
     1981 gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa
     2041 catggccctg agctttaatg atcaagccac tgaaatagtc gatattgctg caaaccaccg
     2101 ggctaatcat cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt
     2161 gccctgcgct tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg
     2221 ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga
     2281 acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt
     2341 tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag
     2401 gaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata
     2461 ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag
     2521 ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt
     2581 ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag
     2641 cacccagacg tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca
     2701 gacgggtgat acaggcgtca attgctttca ggttgtcagt gccgccaacc gcagcaatat
     2761 agttggtcgc cagttgattg aggccttctt cagtgttgct gttggcttct tcagtaacga
     2821 tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac
     2881 tgaacaccac gaagtagata gcgaagaaga caacacccat aaccagcagc atccagacgt
     2941 tctggctggc ggccggcagg ttatacatca acgcgtagtc gatagccccc gcagagaaag
     3001 agaagccagc atggataccc agcagtgttg ccacaaacag gctgataccg gtcagcagtg
     3061 cgtgcaggag gtacagcagc ggtgcaagga acatgaacag gaattccagc ggctcagtca
     3121 caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac
     3181 gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcataa
     3241 tcgggaagaa gccggacatg aacatccccg cggtgccgtc accggcatag aagcggttga
     3301 tatcaccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga
     3361 tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga
     3421 taccggaacc cagcgcgccc gcagaaacga tccactcgcc tcctgcatgg atagcgtgct
     3481 gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag aagccggtgg
     3541 caatcggcac aaagcgtttg ccgccgaaga aactcaggaa gtccggcagt ttaatatcgg
     3601 accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca
     3661 tgttaatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac
     3721 ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga
     3781 agattaatgc gaggttatca aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa
     3841 gtaaatctgg ctgaccgaat cgcagcaaca gtgccgccac cggcagcacc gcgataggga
     3901 gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac
     3961 gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat tgtactgccg
     4021 atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgta
     4081 tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc
     4141 cttttactgg ctaaagcaga aaacttattt tatcattcaa aaaatcaggt cggattgacg
     4201 cctgtctgcg caaatccagg ttacgcttaa agatgcctaa tccgccaacg gcttacattt
     4261 tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa
     4321 tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccgactgc cgatcgtccg
     4381 tttgttctgg gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa
     4441 atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat
     4501 gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc
     4561 gatcacgttg atattccagc agaaaacatc aaccttctca acggcaacgc cccggatatc
     4621 gacgccgaat gccgccagta tgaagaaaaa atctgttctt acggaaaaat tcatctgttt
     4681 atgggcggtg taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct
     4741 tctcgtactc gtatcaaaac cttgactcat gacactcgcg tcgcgaactc tcgtttcttt
     4801 gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacattgctg
     4861 gatgccgaag aagtgatgat tctggtgctg ggtagccaga aagcactggc actgcaggct
     4921 gcagttgaag gttgcgtgaa ccacatgtgg accatcagct gtctgcaact gcatccaaaa
     4981 gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat
     5041 ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggctc
     5101 cttgctcagg gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc
     5161 ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga
     5221 ttaaaagcgt ctgtcctgta gcggaactgc cgccagagat cgaacaacgt tcactgaacg
     5281 gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt
     5341 ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg
     5401 agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga
     5461 aacagggcgt gcgcgttatg cgcgagtacc tggcaaaaca tccgaatcag gcgttaggtc
     5521 tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg
     5581 tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca
     5641 aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg
     5701 ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc
     5761 gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg
     5821 aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attattgctg
     5881 atggcctgca tgttgattac gccaacattc gtaacgctaa acgtctgaaa ggcgacaaac
     5941 tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg
     6001 cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg
     6061 gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac
     6121 tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattggc gttgagaaac
     6181 gtctcggcac actcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta
     6241 aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac
     6301 accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc
     6361 ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag
     6421 ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat
     6481 caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga
     6541 aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca ccatcaccct
     6601 gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca
     6661 gcaaacgctg gaacatgccc tgttgaatgc cattgctcag tttattgata gctaccagcg
     6721 caaactgcgc gagctgatcg cgatttcggt gatcctgcca gggcttgttg acccggacag
     6781 cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact
     6841 ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc
     6901 ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg
     6961 cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca acggcaacgt
     7021 cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt
     7081 tggctgcctg gaaactatcg ctgccaacgc cgctattgaa caacgggtgt tgaatctgtt
     7141 aaagcagggc taccagagcc gcgtgccgct ggacgactgc accatcaaaa ctatctgcaa
     7201 agccgcgaac aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct
     7261 gggcaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgccgg
     7321 tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca
     7381 ggcgctgaag gcgtttcgca ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc
     7441 ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca
     7501 tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag
     7561 tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc
     7621 gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg
     7681 ttgctgacca actatccttc gcagaccggg caagatctgg cgaaccgctt tgccactgcc
     7741 ggtgtcgatg tgccggatag cgtgttttat acctctgcga tggcgactgc cgatttcctg
     7801 cgtcgccagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg
     7861 tacaaagccg gtttcactat taccgatgtg aaccctgatt ttgtgattgt tggcgaaacg
     7921 cgttcctaca actgggacat gatgcataaa gcggcctatt tcgtcgctaa cggtgcacgc
     7981 tttatcgcca ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg
     8041 tgtgcaggga ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg
     8101 tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc
     8161 ggcgataact tgcgcaccga cattctggcg ggcttccagg cgggtctgga gacgattctg
     8221 gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg
     8281 atttacccgt cggtcgctga aatcgacgtt atctgaatag caaccacgtc tcagggcgtg
     8341 gtttcttatt tttacccatc attaaaatca tcccattcaa taaaactcgc caaaaattat
     8401 cgatccatca ataaatacat caccacaccc tccccttttt cacaaatcag caatcaccat
     8461 tacgtttttt atttttgcac tgccaaatcg cttgcgcacg gtgctgcttt gcggcatttt
     8521 tttaaacaag caaacacaac aagcaacaaa taccaggtta acggagaagg ttatgtgttc
     8581 aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga aagcactcga
     8641 gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg ccagcgataa
     8701 cgccattctc gcccacgaac gtctgtcaat tgttgacgtt aacgcggggg cgcaacctct
     8761 ctacaaccaa caaaaaactc atgtgctggc ggtaaacggt gaaatctaca accaccaggc
     8821 actgcgcgcc gaatatggcg atcgttatca gttccagacc ggatctgact gcgaagtgat
     8881 cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg gcatgtttgc
     8941 ctttgccctg tacgacagcg aaaaagatgc atatctgatt ggtcgcgacc atctggggat
     9001 catcccactg tatatgggct atgacgagca cggtcagctg tatgtggcct cagaaatgaa
     9061 agccctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct atttgtggag
     9121 tcaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg atgcagtgaa
     9181 agataacgtg accgacaaaa acgagctgcg tcaggcactg gaagattccg ttaaaagcca
     9241 tctgatgtct gacgttcctt acggtgtgct gctctcgggt ggtctggatt cctcaattat
     9301 ttccgcgatc accaagaaat acgccgcccg tcgcgtggaa gatcaggaac gctctgaagc
     9361 ctggtggccg caattacact cctttgctgt aggtctgccg ggttcaccgg atctgaaagc
     9421 agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact tcactgtaca
     9481 ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacctatg atgtgacaac
     9541 tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcaa tgggcattaa
     9601 aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt acttccacaa
     9661 agcaccgaat gccaaagaac tgcatgaaga gacggtgcgt aaactgctgg ccctgcatat
     9721 gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag cacgcgttcc
     9781 gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg ataaaatgtg
     9841 cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat acctgcccgc
     9901 aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca gttggatcga
     9961 caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag caactggaaa ctgcccgctt
    10021 ccgcttcccg tacaacacgc caacctcaaa agaagcgtat ctgtaccggg agatctttga
    10081 agaactgttc ccgcttccga gcgccgctga gtgcgtgcct ggcggtcctt ccgtcgcgtg
    10141 ttcttccgct aaagcgattg agtgggatga ggcgttcaag aaaatggacg atccgtctgg
    10201 tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa atgcgaaaca gccccggtga
    10261 aatccggggc tgtttcagtt attgaggtgc ttgttaattc tgatatgcag taaggaaatg
    10321 ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta tttcatagat
    10381 taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa agaatggctg
    10441 tagggtgggg aaaaaacaca aagtaaacaa taattgacga atatagcgcc acgctgttcg
    10501 caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt gacgctgcaa
    10561 ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa aaaagatggc
    10621 tacgtagctc agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc
    10681 cgtcgtagcc accatctttt ttgcgggagt ggcgaaattg gtagacgcac cagatttagg
    10741 ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc accagaaagc
    10801 gttgtacgga tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct
    10861 ggttcgaatc caggtacccc agccatcttc ttcgagtaag cggttcaccg cccggttatt
    10921 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
    10981 aggtacccca gccatcgaag aaacaatctg gctacgtagc tcagttggtt agagcacatc
    11041 actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccaaatt ctgaatgtgt
    11101 cgaatacgtt cggcaaattc aaaaaccaat ttgttggggt atcgccaagc ggtaaggcac
    11161 cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccaa tttattcaag
    11221 acgcttacat cgtaagtgcg cccagttggg gtatcgccaa gcggtaaggc accggattct
    11281 gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa agctcgcttc
    11341 ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat gcgatgttga cacgtcttat
    11401 ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggttgg cacgcatccg
    11461 gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt caacacgcca
    11521 gcacgctctg ccgccattaa cccgagatta cgcacaaaac gcagcggcgg cagattatta
    11581 ctgaatccgg cataaaacag atccatacca ctttgcataa tgaagttatc cgccatgcgc
    11641 cgcatctggt aacgcttgag gacaggataa ctggcccacg cttcaccgta gctgcgggca
    11701 ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac cccctgcccc
    11761 gccagcggat ggatggtatg cgcggcatcg cccaccagcg caagccccgg ctgcacatac
    11821 tgcaacgcat gtcggcgcgt cagcggaaac gcaccagcgg caagcggtgt aacgtaaccc
    11881 agacgcgacg ggaaatgctt cgcgatttcc gcctggagtt gcgccatatt catattctgc
    11941 aactggcgaa tacgtaccgg agagtcatac cacaccagcg atgcccagtt atcaaacaac
    12001 ggcagaaacg cacgcggtcc gtccggggta aattgctgcc aggtgctgtc gccgggatcg
    12061 ttctcacact gtacgctaat caacatacac gactgcgcat actgccaggc atgaacgcca
    12121 attcccgcca tctgccgcac ctgcgaattt gcgccgtcag caccaatcac cagcttcgcg
    12181 cgaatcactt caccgccctt cagttccagc tcctgaagat cattatctcg atgtaatgca
    12241 atcagcgagc ctggcacacg taacgttact ttcggatgcg cttccagcgc ctgccacaat
    12301 gcctgttgca ggacggtgtt ttccaccata tagccaagca gcggcagctt aagttcagcg
    12361 gcgtcaaaca tcacatgcgc cgtttcccac tcccacgttt ccagtctgcg gtaaggatgg
    12421 caacgcatag cctgtactgc atcccagacc cctaaccctt taagcaatga taccgatgcg
    12481 gcgctgatcg ccgagatccg cacgtccggt tggctatcag cgacaaacgg tgcaggttct
    12541 gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca gccccagcgc cagtgcgccg
    12601 ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat ggtcaatcat
    12661 cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg ataacgctca
    12721 tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg gctactattt
    12781 cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg tcagatgaac
    12841 gagtacgatt catcgaagat ggccgatctg ctggatgcca cccacggcta tcaactgacc
    12901 gacgtggcgg aagaagcgga tgtgctgctg ctgaacacct gctcaatccg cgagaaggct
    12961 caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa gaatccagac
    13021 ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat tcgccagcgc
    13081 gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc ggagatgatc
    13141 aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga aatcgagaag
    13201 tttgaccgtc tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc catcatggaa
    13261 ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga agaggtaagc
    13321 cgtccgtccg acgatattct gtttgagatc gcccagcttg cggctcaggg cgtgcgtgaa
    13381 gtcaacctgc tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga cggcaccacc
    13441 ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga tcgtattcgc
    13501 tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta tcgcgacacg
    13561 ccggagctgg tgagcttcct gcatctgccg gtacagagcg gttccgatcg cattctgaac
    13621 ctgatgggcc gtactcatac ggcactggag tacaaagcga tcatccgtaa actgcgtgcg
    13681 gcgcgtccgg atattcagat cagttctgac ttcattgttg gcttccctgg cgaaaccacc
    13741 gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat gagctacagc
    13801 tttatcttct ctgcacgtcc aggaacgcct gccgctgata tggttgatga tgttccggaa
    13861 gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca agcgatggca
    13921 tggagccgtc ggatgctcgg caccacccag cgtattctgg tagaaggtac atcgcgtaag
    13981 agcatcatgg agctttccgg gcgtaccgaa aataaccgtg tggtcaactt cgagggcaca
    14041 ccggatatga tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc gaactctctg
    14101 cgcggtaaag tggtgcgtac tgaagatgaa atgggtctgc gcgtggcaga aacacccgaa
    14161 tcggtgattg cccgtactcg caaagaaaat gaccttggcg tgggttatta tcagccgtaa
    14221 ttctcaggcc cgccgttccg gtgggccttg ttttttctct ctttatcccc atctttatcg
    14281 caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg tgaatgttca
    14341 gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc gaaatcaccc
    14401 tggagccagc agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat gacaacatca
    14461 agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgacaaccac tttaaactga
    14521 ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg tatgtcgata
    14581 cagccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac cttgcgatta
    14641 aagaagcgcg agtactggag caaagtgcgg agagcgtgcc ggagtacggc aaagcggtca
    14701 atatcaaaac caaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg cagtacatcg
    14761 ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg ggtaaaacct
    14821 acctggcagt ggctgcggca gttgatgccc tggagcgtca ggagattcgc cgtattctgc
    14881 tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt cctgcctggc gatttaagcc
    14941 agaaagtaga cccgtatctg cgcccgctgt acgacgcgct gtttgaaatg ctgggctttg
    15001 agaaagttga gaaactgatt gagcgcaacg ttattgaagt cgcgccgctg gcctatatgc
    15061 gtggtcgtac gctgaacgac gcgtttatca ttctcgatga gagccagaac accaccatcg
    15121 aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt atcaccggcg
    15181 acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcac gccatcgaag
    15241 tgctggccga tgtcgaagag atcagcttta acttcttcca cagcgaagac gtggttcgtc
    15301 acccggtggt ggcgcgtatc gttaacgcct atgaagcctg ggaagaagcc gaacaaaaac
    15361 gaaaagcggc gctggcggca gaacgcaagc gcgaagaaca ggaacaaaaa tgagtcaggt
    15421 gatcctcgat ttacaactgg catgtgaaga taattctggg ttaccggaag agagccagtt
    15481 tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa gaatcggaag tgactattcg
    15541 cgtggtcgat accgccgaaa gccacagtct gaatctgacc tatcgcggta aggataagcc
    15601 gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc atggaaatgt cgctactggg
    15661 cgatctggtt atctgccgtc aggtggttga gaaggaagct caggagcaag gcaaaccact
    15721 ggaggcgcac tgggcgcata tggtggtgca cggcagtctg catttgttag gttacgatca
    15781 catcgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta tgcttgctct
    15841 gggctatgag gatccgtaca ttgccgagaa agaataattt gccaggcatt tgactggcag
    15901 acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga gaacccatac
    15961 gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa gaagggattt
    16021 ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga cgaactgctg
    16081 gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg cgatatgctc
    16141 gaaggggtga tggacatcgc agaccaacgc gttcgcgaca tcatgatccc ccgctcccag
    16201 atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat catcgagtcc
    16261 gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg gattctgatg
    16321 gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcagcat ggacaaagtg
    16381 ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct gaaagagttt
    16441 cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt ttccggtctg
    16501 gtgaccattg aagacatcct ggaactaatt gttggtgaga ttgaagacga gtatgacgaa
    16561 gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg cgcactggct
    16621 tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga ggttgacact
    16681 atcggtggtc tggtgatgca ggcatttggg catcttccgg cgcgtggcga aactatcgac
    16741 atcgacggtt accagttcaa agtggcgatg gccgacagtc ggcgtattat tcaggttcat
    16801 gtcaaaatcc cggatgactc accccagccg aagctggatg aataaaaccg aaactggata
    16861 gataactaca tggcttttgc ctcattaatt gaacgccagc gcattcgcct gctgctggcg
    16921 ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgatgtctg gcctgcggcg
    16981 attatttcgc tgatggggct tcaggcgttg acctttaacc gccgtccact ccagtctgcc
    17041 gctattggct tttgctgggg atttggcctc tttggcagcg gtattaactg ggtctatgtc
    17101 agcatcgcga cctttggcgg gatgcctggc ccggttaaca tcttcctggt ggtgctgctg
    17161 gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcgcg tctgtggccg
    17221 aaaaccacct ggctgcgcgt ggcgattgcc gccccttctc tctggcaagt gaccgagttt
    17281 ctgcgcggtt gggtactgac aggcttcccg tggttacagt tcggctatag ccagattgat
    17341 ggcccgttaa aagggctggc accgataatg ggcgtggaag ccattaactt cctgctgatg
    17401 atggttagtg gcctgctggc actggcgttg gtcaaacgca actggcgtcc gctggtggtg
    17461 gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca tccaatggtt taccccacaa
    17521 ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc actgaaatgg
    17581 gatggagatc agcttcttaa tacgctgaag atttactaca acgcaacggc tccgctgatg
    17641 ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga aattaatcag
    17701 caaccgttcc tcaaagcact ggacggtgag ttgcgcgata aaggtagctc gctggtaacc
    17761 gggattgtcg acgcgcgtct caataagcag aaccgctacg atacctacaa caccatcatc
    17821 acgctgggta aaggtgcacc gtacagctac aaatcagccg atcgctataa caaaaaccat
    17881 ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt agcaccgttc
    17941 tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc gctgtcggca
    18001 aatggtattg agcttactgc ggctatttgc tacgagatca ttctcggcga gcaagtgcgc
    18061 gataacttcc gcccggatac cgactatctg ctgactatct ccaacgatgc gtggtttggt
    18121 aagtctattg gtccatggca gcacttccag atggcgcgaa tgcgtgcgct ggagctggcg
    18181 cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc gcagggtgag
    18241 attcaggcga tgatcccgca gttcacccgc gaggtgttga ccactaatgt gacaccgacc
    18301 accggactca caccgtatgc acgtaccggc aactggccgc tgtgggtgct gacggcgctg
    18361 tttggttttg ccgctgtgct gatgagtctg cgagcacgta aacgttgatg tgcctgatgt
    18421 atcctgcatg agcaaaacgt actttctcat taagctttgg ggcatggtta tcatgcccct
    18481 ttttcttacg gttcagggat acaagccaca ttgccatatt ggggatgacg aataattgcc
    18541 tgcggatcaa gcgagcgcgt cctgtccatt tggatcaacc cgttcttcgc catgacaaat
    18601 atttttctac catccgctga aaaacgaagg tgattaacgt agctggcagg ataaacgcca
    18661 ataatctgcc cggcattaat attgagcagg atgacataac cttgcagtcc atatgacacc
    18721 aacgctaaat attgaccgtc cgggctaata ccctcaacgg aataaacgct cctgctacac
    18781 cattgcgtca taatgctggt ttgttgatcc cctatgctta tacgtgaaat cccctgacta
    18841 tcggcaaaaa taacctcgcc gttttcaggt cgaaaacgca tcgccgatat cgttccacgc
    18901 gcatcacctg cgggaaagtc ttctttctga tgcgtgatgg tattgatccg cacgatgtta
    18961 cctctgcctt ctcccggaac cagtaatgtt ctttcatccg gggaaagtgc cataacccag
    19021 gcgcctggtt taccgctcgt ccccgtcccg tcgtatttct ccagcgggat atccgccaca
    19081 tgctgataag ccaccagatc aatggctgac acactattac tggtgcgatt catcacccag
    19141 gcttttgtgc catcacgcga gaggataaga ctgtcaggat gtgcaccggt ggtaatcgac
    19201 tgaataatct cccctgtcgc cacgttaatg accgataacg cattcgcttc aaatgaagtc
    19261 gcataaatca acttttcatc cgggcttaat gccagcgatc tgacgccctg atctttacag
    19321 ccaccacaat gtttgactga cttttctttc gctatgggcg ctgtaaaggt atgcaggatt
    19381 ttcttttgtc cgaggtcgaa aacaacaatg ctgttggtgt cttcaattgc ggcatacaac
    19441 gtattaccat cccgactgat cgccatatca tggatatcgg caatctcagc gagcaaatca
    19501 ccactggcct gttcacgact taccggagcg gataatgttt ccctgagggc gttgcgttcc
    19561 tccgctaact gctgcttttt ttgccgtcgc tcttgttcaa tctcctgtgg ataaaacgct
    19621 tcacgcaggc tttgcctgac ttgtaaaact gactcatcca tctgctgctg cgcaagccag
    19681 atagccccgg tcagaagaag actgcccgca acaataatcc cgccttccgt aacgagtcgg
    19741 ttttgcccag ataaacgcgc gctcagccaa cccaacgcgc cacccgcaat caggccgcca
    19801 atgtggcaag cgttatctat tcctgtttgc cgggcaccgt acaacagtgt cagcgccacc
    19861 atacccaaca gaggaaatac tcgccgctgg tttttatggt atgtgcccgt acctgaagca
    19921 aactgtgttg ctatcgctgc accagctatt cccagaatcg ccccggatgc cccaacgctg
    19981 atgactatct gctcactttc gcgtaacgca taacaggcgc tgatgagccc accaaagacg
    20041 ccggagaata accaggtaat caggagtcgc catttcccca gaatctgttc cgccacgcct
    20101 cccacgacca ggaatgcaaa catattcatc agcagatgag aaacactact gtgcagaaag
    20161 atactgctca ataagcgcca gggttcaccc gacaaagtaa gaggcgcaac gtttgcgccg
    20221 agatggataa gtgcggattg atcgacctct tcataactca tccattgtgc gagataacaa
    20281 aatatcccaa tacagagtgc aaaaaggata agggttattg cgggtaattg aacatttaac
    20341 ggcttaaccg aagatgcaga cataaggtgt ttcttccttg aaaagacagg taaaaagcat
    20401 ttttcccgtg tgcagttatc atcccggcaa acgtcccatg ccgaaaagat aattctgtcc
    20461 gaaatatgac tttttcagcc taaaattaca atcgtaatta attcgcccct aagtattttt
    20521 tggcatgtaa gttgctttat ttactgtgta agcgatgaaa tcagccgcga cgcaaccagg
    20581 ttgcaccacc acaaatcagc ggtagcacca aaaaggtgcg gtttaagaat tttgcttcgg
    20641 aatggtgcgg cgcatctcgc aaaaacaaac aataaaacag caatatcttt acatttagcc
    20701 aaactaaatg ttacctaaca ggcacaacac tgcacaataa agttgcagac gataacaaca
    20761 caaacactca caacgggtat ccatgcgttc ttaacgcaga agataaagga gttggatatg
    20821 caattacgta aacctgccac agcaatcctc gccctggcgc tttccgcagg actggcacag
    20881 gcagatgacg ccgccccggt agcgggcagt acgctggaca aaatcgccaa aaacggtgtg
    20941 attgtcgtcg gtcatcgtga atcttcagta cctttctctt attacgacaa tcagcaaaaa
    21001 gtggtgggtt actcgcagga ttactccaac gccattgttg aagcagtgaa aaagaaactc
    21061 aacaaaccgg acttgcaggt aaaactgatt ccgattacct cacaaaaccg tattccgctg
    21121 ctgcaaaacg gcacttttga ttttgaatgt ggttctacca ccaacaacgt cgaacgccaa
    21181 aaacaggcgg ctttctctga cactattttc gtggtcggta cgcgcctgct gaccaaaaag
    21241 ggcggcgata tcaaagattt tgccgacctg aaagacaaag ccgtagtcgt cacttccggc
    21301 actacctctg aagttttgct caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc
    21361 atcagcgcca aagatcacgg tgactctttc cgcaccctgg aaagcggtcg tgccgttgcc
    21421 tttatgatgg atgatgccct gctggccggt gaacgtgcga aagcgaagaa accagacaac
    21481 tgggaaatcg tcggcaagcc gcagtctcag gaggcctacg gttgtatgtt gcgtaaagat
    21541 gatccgcagt tcaaaaagct gatggatgac accatcgctc aggtgcagac ctccggtgaa
    21601 gcggaaaaat ggtttgataa atggttcaaa aatccaattc cgccgaaaaa cctgaacatg
    21661 aatttcgaac tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaggcactg
    21721 aactaattac aagaaccagg ggcggaaatt ccagccctct cgattgttac gtagcacgga
    21781 cagactatac gcctgatggt cgttccccat cgggcctgaa aaccgcaata cgctgggtaa
    21841 caatcttcga gggtagcagt taacgctgct accctctttt ttctggagta gatttatgtc
    21901 tatagactgg aactggggta tttttttaca acaagccccg ttcggcaaca ccacctatct
    21961 tggttggatc tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg cctggattat
    22021 cgctttcctc gtcggttcat ttttcggcat tttacgtacc gttccaaacc gttttctctc
    22081 cggtctgggt acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg tgcaattctt
    22141 tacctggtat ctggtgatcc cggagctgtt gccggagaaa atcggcatgt ggtttaaggc
    22201 cgagctggat cccaatattc agtttttcct ttcatccatg ctctgcctgg ggctgtttac
    22261 cgccgcccgt gtttgcgaac aggtacgcgc cgctattcag tcgctcccgc gtgggcagaa
    22321 gaatgccgcg ctggcgatgg gcctgacgct accgcaggct taccgctatg tactgctgcc
    22381 taatgcttat cgcgttatcg tcccgccgat gacctccgag atgatgaacc tggtgaaaaa
    22441 ctccgccatc gcctcgacta tcggtctggt ggatatggca gcgcaggcgg gtaaattgct
    22501 cgactactcg gctcacgcct gggagtcgtt taccgccatt acgctggcgt atgtattgat
    22561 taacgcggtt atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc tgcccggcaa
    22621 catggggggc aaataatgta cgagtttgac tggagttcca ttgttccttc cctgccatat
    22681 ctgctcgacg ggctggtgat cacgctgaaa atcaccgtca cggcagtcgt gattggtatt
    22741 ttgtggggca ccatgctggc ggtgatgcgc ttgtccagct ttgcgcccgt agcgtggttt
    22801 gccaaagcct acgttaacgt attccgctcg attcctttag ttatggtttt gctgtggttt
    22861 tacctgatcg tgccgggttt tctgcaaaac gtgctgggat tatcgccaaa aaatgatatc
    22921 cgtttaattt cggcgatggt ggcgttttcg atgtttgaag cggcctacta ttcagagatt
    22981 atccgtgctg gtattcaaag tatttctcgt ggtcagtcga gcgccgcgct ggcgttggga
    23041 atgactcact ggcagtcgat aaaactgatt attctgccgc aggcgttccg cgcgatggtg
    23101 ccgcttctgc tcactcaggg catcgtactg ttccaggata cctcactggt gtatgtgtta
    23161 tctctggccg atttcttccg taccgcctca accattggtg agcgtgatgg tacgcaggtt
    23221 gagatgatcc tgtttgccgg atttgtttat ttcgttatta gtcttagcgc gtcgttgttg
    23281 gtcagctact tgaaaagaag gacagcataa tgattaccct gaaaaatgtt tcaaaatggt
    23341 atggtcactt tcaggtgctg accgactgct caaccgaagt gaaaaaaggc gaagtggtgg
    23401 tggtttgcgg cccgtctggc tccggcaaat caacgctgat taaaaccgtc aacggcctcg
    23461 aaccagtgca gcaaggtgaa atcaccgtcg atggtatcgt ggttaatgac aagaaaaccg
    23521 atctggcaaa gctgcgttcc cgcgtcggga tggtgttcca gcattttgag ttgttccctc
    23581 atctgtcgat tatcgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa cgcgataaag
    23641 cgccggctcg tgaaaaagca ttgaaattgc tggagcgcgt gggactttcc gcacatgcca
    23701 ataaatttcc ggcgcagctt tccggcggtc agcagcagcg tgtggcgatc gctcgcgcat
    23761 tgtgtatgga tcctattgcg atgctgttcg acgaaccgac gtcggcgctg gatccggaga
    23821 tgatcaacga agtgctggac gtgatggtgg aactggcgaa cgaaggaatg accatgatgg
    23881 tggtgaccca cgaaatgggc tttgcccgca aagtggcgaa tcgggtgatc tttatggatg
    23941 agggtaaaat tgtcgaagac tcgccgaaag acgctttctt cgatgatccg aaatcggacc
    24001 gcgcaaaaga cttcctcgcg aaaatcctgc attaatcact ctggcgcgca tacccaagtg
    24061 cgcgccatct cacgctttat cgtttcgccc ctcgtatctg ttacatcctg gcgttaccct
    24121 tgtcgcaaag aagcacacaa caaggagcaa caatggcact gccaattctg ttagattgcg
    24181 acccaggtca tgacgacgct atcgcaatag ttctcgccct cgcctcacca gagcttgatg
    24241 tcaaagcaat tacgtcttcc gccggaaacc agacaccaga aaaaacctta cgcaatgttc
    24301 ttcgtatgct gaccttgctc aatcgcaccg atattccggt agcaggcggc gcggtaaaac
    24361 cgttaatgcg tgagttgatt atcgcggaca atgtgcacgg cgaaagcggt ctcgacggcc
    24421 cggcattacc ggaaccggcg ttcgcaccgc aaaactgtac ggcggtagag ctaatggcga
    24481 aaacgctgcg tgaaagtgcg gaacctgtca cgattgtgtc taccggaccg caaactaacg
    24541 ttgccttgct gctcaatagc catccggaac tgcatagcaa aattgcccgt atcgtgatta
    24601 tgggcggcgc aatggggctt ggtaactgga cgcctgcggc tgaatttaat atttacgttg
    24661 acccggaagc ggcagaaatt gtcttccagt cagggatccc ggtggtgatg gccggtctgg
    24721 atgttactca taaagcacaa atccacgttg aagacaccga gcgtttccgc acgattggca
    24781 accctgtttc aaccattgtt gccgaactgc tggatttctt cctcgaatat cataaagacg
    24841 aaaaatgggg ctttgtcggt gcaccgctgc atgacccatg caccatcgcc tggctgttga
    24901 aacccgagtt gtttaccact gttgagcgct gggttggcgt ggaaacacag gggaaatata
    24961 gccagggtat gacggttgtt gattattatt atctgactgg caataaaccg aatgccactg
    25021 taatggtcga tgttgatcgt cagggctttg ttgatttact ggctgatcgt ctgaaatttt
    25081 acgcttaaca cacgtcattg attaagatca ccacgcgtta cctgggtggt cttaatctgt
    25141 ttatctcttt ttctttcaag atataaatgc aatggataat gcagaactcg ccattggtat
    25201 cgatctcggc actaccaata gtttaattgc cgtctggaaa gacggtgccg cgcaattaat
    25261 tccaaataag ttcggtgaat atttaacacc atccataatt agcatggatg aaaataatca
    25321 tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg catccggata aaacggcagc
    25381 gttatttaaa cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg acacatttaa
    25441 cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa gaagatgccg aagaatttct
    25501 gcaacgtccg attaaagatg tggtgatctc cgttccggct tatttcagcg atgaacaacg
    25561 caagcatacc cgtttagcag cggagttagc cgggttaaat gcggtacgct taattaatga
    25621 acccacagca gctgcgatgg catatggcct gcatacgcaa caaaataccc gctcgctggt
    25681 ttttgatctc ggtggcggca cgtttgacgt cacggtgctc gaatacgcca cgccggtgat
    25741 tgaagttcac gcctccgctg gcgataactt cctcggtggc gaagatttta cccatatgtt
    25801 ggtcgatgac gttttaaagc gcgcggatgt cgccaggacc acgcttaacg agagtgaact
    25861 ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc cattgcacat
    25921 tcgctggcag taccaggatg aaacgcggga gtgcgaattt tacgagaacg aactggaaga
    25981 tttgtggttg ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt tgcgcgatgc
    26041 acgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt cacaaatgcc
    26101 gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa gttacgaccc
    26161 gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac gcagtgaaga
    26221 tattgaagag gtgatcctca ctgatatttg cccttactcg ttgggcgttg aagttaaccg
    26281 ccagggcgtt tccggcattt tctcgccaat tattgaacga aacaccacag tacccgtgtc
    26341 gcgtgtagaa acttactcga ccatgcaccc ggaacaggat tcaattacgg ttaacgtcta
    26401 tcagggagaa aaccacaaag ttaaaaataa cattctggtt gaatccttcg atgtgccgtt
    26461 gaagaaaacc ggggcttatc agtcgattga tattcgcttt agttatgata tcaacggctt
    26521 gcttgaagtt gacgtgcttc tggaagatgg cagcgttaag tccagagtca ttaatcatag
    26581 cccggtaaca ttgagcgcgc agcagattga agagagtcgg acgcggttat ccgcattgaa
    26641 aatttatccg cgcgatatgc tcatcaaccg cacctttaag gccaaactgg aagagttgtg
    26701 ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg gtgatcaccg attttgatgc
    26761 ggcgttgcag tcaaacgata tggcccgcgt cgatgaggtc aggcggcggg cgagcgttta
    26821 tttagccatt gagacctcat aatcacggct gccggatgcg gcgtaaatgc tgcatccggc
    26881 atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg attttgccga gagcataacc
    26941 tataaccagc accgttaaac tgattttgat aatgctggta atgctctgtg gtttttcacc
    27001 ctcatcattt ttttcttcat ggttttcagg tgttggctca tcgacatctt catcctccgg
    27061 ctcgagtttt actttcgtca acagcatccg ggtgataatg gcctctaatg gatgagcatc
    27121 tccgccttcc agccttgtat taagacgggc cagcatctct tgtttcggaa acgcttcaag
    27181 tactacctgt tccgctaccc gatacaaatc cagtaacggt gaatcttctt ctgtctcgct
    27241 accgactctc gatttccacc acgcccatgc tttgcgtttg aagaataacg actcaataaa
    27301 cgattgacct aacggcgata attttccgct atgtaaaatt tccgcccata acagccgcgt
    27361 ccgggcgctc tcaccaggaa catatttcag cggttgacct tgcgcatcta ccatgctccg
    27421 cgcttcgata accatcacta ataacgcgaa ataatccggg caatgactct tgctccactg
    27481 caacaacaaa ttatcagctt gtgtagatgg gtaactttcc cggtacgcgc agagatcggc
    27541 aagcaggctt tccccctcgc cgcagtacaa cctttgagca cacaagtaat atcgggcatc
    27601 ttcgctttct ggttcttcca tttgccactg tttggctaca ggaatgagtt ccgccatacc
    27661 tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt aactttgcat catcaggaat
    27721 gaaccacgga ccttccatcg ccaaaaacgc agtgacgtag tcaggatggt aacggcagca
    27781 acgttccagc gcgaagtaat actcaacagt ctggttctgc aacgcaacgg gatattccag
    27841 aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag cctccctttc
    27901 ttcctcatcc attccttcgc tgtcattaaa cgaatgccag ttcaaacgct ctgccagcaa
    27961 agaagcacaa tttaatgaaa gatatcgcgc ttctatagcg attgcacaca gcggccagcg
    28021 taattgatcg acatcctcca tgttccaggt atttaattgc tgaataaatt tctgccaggc
    28081 ggagggctga aaacgatcac tttctgaatc cagtaatgtc ctgaatgcac gcagtatttc
    28141 atgttcagcg gcggcatctt tttcttcatc atctgcttcc tcgacaggat ttaccgccag
    28201 tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac ccctgcggat cggtttcggg
    28261 atgacataac ggtaagcgag caagataagc ctggcggata atgtctattt gcgtcgtgct
    28321 ttcaatttcg aggatttgcc aacatgtttt cataggtcgc tcacatcctt aagcgttacc
    28381 gggcattcag gctggacagt taatggctgc cagggaagcg tggcatagaa acgatcgctt
    28441 cccgctgcca gctcttcgaa catccgactc ccggcgctat cgtatttgta cagggttagc
    28501 gcctcgcgta gcgaagtgtt gtcttcgcac tgagaagatt gccagtagag acgtcctaac
    28561 gaataggcgg aaaaatagtg catccagctg tgatagtaat ggtgagctcg tacatagacg
    28621 cgcgattgca gccataaact atcttcttcc gtcagccagt tgttgagcac accgacccgt
    28681 gacagaaacc ccattcgaac ataatcccag gctttgattc ccccttctcc acagcacata
    28741 gcggtttggg caacgaattg ggtataaatc aaccctctct ccgagccacc agcaattaat
    28801 gctttccact cttcagggga agagcgatgc catgtcagat ataagccagc taaatcattg
    28861 gcatgaccat catcagtcat ctgataaata gtctgaatta aatcatcacg attatttatt
    28921 ccccaggatg atgagagatc gaattctttt ataccggtat acatttttgg cgagtcatgc
    28981 gcgggtttgc aaccctgatt aagtatttcc atgggcgcag aaagcgcaaa aagaagatat
    29041 tgttcttcct tattcatttg ttaataatcc cttaagtgca ttaactgact actttttatt
    29101 attgatgagt ataaacttga cgctttc
//
LOCUS       DAZFYC010000058        29029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFYC010000058 DAZFYC010000000
VERSION     DAZFYC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.058"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            209..1075
                     /gene="folD"
                     /locus_tag="ACHKW5_002761"
     CDS             209..1075
                     /gene="folD"
                     /locus_tag="ACHKW5_002761"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO7512123.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1077..1289
                     /gene="ybcJ"
                     /locus_tag="ACHKW5_002762"
     CDS             1077..1289
                     /gene="ybcJ"
                     /locus_tag="ACHKW5_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO7512124.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1397..1918
                     /gene="ybcI"
                     /locus_tag="ACHKW5_002763"
     CDS             1397..1918
                     /gene="ybcI"
                     /locus_tag="ACHKW5_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7512125.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1954..3339)
                     /gene="cysS"
                     /locus_tag="ACHKW5_002764"
     CDS             complement(1954..3339)
                     /gene="cysS"
                     /locus_tag="ACHKW5_002764"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO7512126.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3513..4007
                     /gene="ppiB"
                     /locus_tag="ACHKW5_002765"
     CDS             3513..4007
                     /gene="ppiB"
                     /locus_tag="ACHKW5_002765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO7512127.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4010..4732
                     /gene="lpxH"
                     /locus_tag="ACHKW5_002766"
     CDS             4010..4732
                     /gene="lpxH"
                     /locus_tag="ACHKW5_002766"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO7512128.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKAEALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4850..5359
                     /gene="purE"
                     /locus_tag="ACHKW5_002767"
     CDS             4850..5359
                     /gene="purE"
                     /locus_tag="ACHKW5_002767"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO7512129.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5356..6423
                     /gene="purK"
                     /locus_tag="ACHKW5_002768"
     CDS             5356..6423
                     /gene="purK"
                     /locus_tag="ACHKW5_002768"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO7512130.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6560..7453)
                     /gene="ybcF"
                     /locus_tag="ACHKW5_002769"
     CDS             complement(6560..7453)
                     /gene="ybcF"
                     /locus_tag="ACHKW5_002769"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7512131.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKAYGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7450..8265)
                     /gene="ylbF"
                     /locus_tag="ACHKW5_002770"
     CDS             complement(7450..8265)
                     /gene="ylbF"
                     /locus_tag="ACHKW5_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7512132.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGTP"
     gene            complement(8276..9535)
                     /gene="ylbE"
                     /locus_tag="ACHKW5_002771"
     CDS             complement(8276..9535)
                     /gene="ylbE"
                     /locus_tag="ACHKW5_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7512133.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9545..11212)
                     /gene="fdrA"
                     /locus_tag="ACHKW5_002772"
     CDS             complement(9545..11212)
                     /gene="fdrA"
                     /locus_tag="ACHKW5_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7512134.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEMLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVACDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11529..12578
                     /gene="allD"
                     /locus_tag="ACHKW5_002773"
     CDS             11529..12578
                     /gene="allD"
                     /locus_tag="ACHKW5_002773"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO7512135.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12600..13835
                     /gene="allC"
                     /locus_tag="ACHKW5_002774"
     CDS             12600..13835
                     /gene="allC"
                     /locus_tag="ACHKW5_002774"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO7512136.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDICIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYWVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13846..14631
                     /gene="allE"
                     /locus_tag="ACHKW5_002775"
     CDS             13846..14631
                     /gene="allE"
                     /locus_tag="ACHKW5_002775"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO7512137.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14760..15905)
                     /gene="glxK"
                     /locus_tag="ACHKW5_002776"
     CDS             complement(14760..15905)
                     /gene="glxK"
                     /locus_tag="ACHKW5_002776"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO7512138.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(15927..17228)
                     /gene="ybbY"
                     /locus_tag="ACHKW5_002777"
     CDS             complement(15927..17228)
                     /gene="ybbY"
                     /locus_tag="ACHKW5_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO7512139.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17285..18646)
                     /gene="allB"
                     /locus_tag="ACHKW5_002778"
     CDS             complement(17285..18646)
                     /gene="allB"
                     /locus_tag="ACHKW5_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO7512140.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18706..20160)
                     /gene="ybbW"
                     /locus_tag="ACHKW5_002779"
     CDS             complement(18706..20160)
                     /gene="ybbW"
                     /locus_tag="ACHKW5_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO7512141.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFCLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20330..21208)
                     /gene="glxR"
                     /locus_tag="ACHKW5_002780"
     CDS             complement(20330..21208)
                     /gene="glxR"
                     /locus_tag="ACHKW5_002780"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7512142.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21308..22084)
                     /gene="hyi"
                     /locus_tag="ACHKW5_002781"
     CDS             complement(21308..22084)
                     /gene="hyi"
                     /locus_tag="ACHKW5_002781"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO7512143.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(22097..23878)
                     /gene="gcl"
                     /locus_tag="ACHKW5_002782"
     CDS             complement(22097..23878)
                     /gene="gcl"
                     /locus_tag="ACHKW5_002782"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO7512144.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAKRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(23968..24783)
                     /gene="allR"
                     /locus_tag="ACHKW5_002783"
     CDS             complement(23968..24783)
                     /gene="allR"
                     /locus_tag="ACHKW5_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO7512145.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24861..25343)
                     /gene="allA"
                     /locus_tag="ACHKW5_002784"
     CDS             complement(24861..25343)
                     /gene="allA"
                     /locus_tag="ACHKW5_002784"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO7512146.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25573..26499
                     /gene="allS"
                     /locus_tag="ACHKW5_002785"
     CDS             25573..26499
                     /gene="allS"
                     /locus_tag="ACHKW5_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO7512147.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPFGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26568..27662
                     /gene="mnmH"
                     /locus_tag="ACHKW5_002786"
     CDS             26568..27662
                     /gene="mnmH"
                     /locus_tag="ACHKW5_002786"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO7512148.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27778..28143
                     /gene="ylbG"
                     /locus_tag="ACHKW5_002787"
                     /pseudo
     CDS             27778..28143
                     /gene="ylbG"
                     /locus_tag="ACHKW5_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28910..29029)
                     /locus_tag="ACHKW5_002788"
                     /pseudo
     CDS             complement(28910..29029)
                     /locus_tag="ACHKW5_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7167 a         7844 c         7192 g         6826 t
ORIGIN      
        1 aaggaaataa atcagttcca gatttacaac gccatcatgg acgaaaaatg aagctttcaa
       61 tctcagcgac ctcagcgacg gtgcgcctaa ccttcgcaag aggtcgcttc acgcgataaa
      121 tctgaaacga aacctgacag cgcgccccgc ttctgacaaa atagacgcat ccccttcgac
      181 ctacgtaaca gatggaatcc tctctctgat ggcagcaaag attattgacg gtaaaacgat
      241 tgcgcagcag gtgcgctctg aagttgctca aaaagttcag gcgcgtattg cagccggact
      301 gcgggcacca ggactggccg ttgtgctggt gggtagtaac cctgcatcgc aaatttatgt
      361 cgcaagcaaa cgcaaggctt gtgaagaagt cgggttcgtc tcccgctctt atgacctccc
      421 ggaaaccacc agcgaagcgg agctgctgga gcttatcgat acgctgaatg ccgacaacac
      481 catcgatggc attctggttc aactgccgtt accggcgggt attgataacg tcaaagtgct
      541 ggaacgtatt catccggaca aagacgtgga cggtttccat ccttacaacg tcggtcgtct
      601 gtgccagcgc gcgccgcgtc tgcgtccctg caccccgcgc ggtatcgtca cgctgcttga
      661 gcgttacaac attgatacct tcggcctcaa cgccgtggtg attggcgcat cgaatatcgt
      721 cggtcgccca atgagcatgg aactgttgct cgcaggttgc accactaccg tgactcaccg
      781 cttcactaaa aatctgcgtc atcacgtaga aaatgccgat ctgttgatcg ttgccgttgg
      841 caagccaggc tttattcccg gtgactggat caaagaaggc gcaattgtga ttgatgtcgg
      901 catcaaccgt ctggaaaatg gcaaagttgt gggcgacgtc gtgtttgaag acgcggctaa
      961 acgcgcctca tacattacgc ctgttcccgg cggcgttggc ccgatgacgg ttgccacgct
     1021 gattgaaaac acgctacagg cgtgcgttga atatcatgat ccacaggatg agtaacatgg
     1081 cgacattttc tttaggtaaa catccgcacg ttgagctgtg cgacttgctg aaactggaag
     1141 gctggagcga aagcggcgcg caggcgaaaa tcgcgattgc cgaaggccag gtgaaagtcg
     1201 acggtgcggt tgaaacgcgc aaacgctgca aaatcgtcgc cggtcagaca gtgagttttg
     1261 caggtcacag cgtacaggtt gttgcctgat ccctttcccg gcctgtatca acaggccggt
     1321 tctcttttca gacttttgcc ctctacgccc cgttcatcca tgctgatagc atcgacattt
     1381 tgccgaagcg accatcatgc caaccgttat tacgcacgcc gccgttcccc tatgcattgg
     1441 tttagggttg ggttcgaaag tcatccctcc gcgtctgtta tttgccggaa tcatcctggc
     1501 gatgctgccc gacgccgacg tattgtcgtt taaatttggc gttgcttacg gcaatgtttt
     1561 tggtcatcgc gggtttaccc attcgctggt gtttgcgttt gttgtcccgc tgttatgtgt
     1621 gctgattgga cgccgatggt tcagggctgg gctgattcgc tgctggctgt ttttaaccgt
     1681 ctcgttgcta tcgcacagct tgctggattc ggtaaccact ggcggtaagg gcgttggctg
     1741 gctgtggccg tggtcagatg aacgcttttt cgctccctgg caggtgatta aagtcgcgcc
     1801 gtttgcattg tctcgttaca ccacgccgta cgggcatcag gtgataattt ctgaattgat
     1861 gtgggtgtgg ttgccgggga tggtgcttat gggaatgttg tggtggcgca ggcgataacc
     1921 ggatgcgaaa actcgcatcc ggcaacagcg caattactta cgacgccagg tggtcccttg
     1981 cgggccatct tccagcacga tccccatctc gttaagacgg tcacgcgccg catctgccgc
     2041 cgcccagtct ttcgctttac gggcatccag acgctgttga attaacgctt caatctcagc
     2101 cacttcgctg tcgtctgcct gcgcgccgct ttgcaggaac gcttccggtt cttgctccag
     2161 caggcccaat acagcggaaa gtttacgcag gtgagatgcc attgcattcg ccgctgccat
     2221 atcttctgct ttcagacggt ttacttcacg cgccatatca aacagtacgg aataggcttc
     2281 cggggtgttg aaatcgtcgt ccatcgcttc aataaagcgc gcttcaaacg cttcgccacc
     2341 ggcaggcgca acggttttat ctgtgccgcg cagcgcagtg tagagacgct ccagcgccgc
     2401 acgcgcctgc ttcaggttct cttcgctgta gttcaactgg ctgcgatagt ggcccgacat
     2461 caggaagtaa cgcacggttt ccgcgtcgta gtatttcagc acatcgcgca cggtaaagaa
     2521 gttacccagc gatttggaca tcttctcgcg gtcaaccatc accatccccg agtgcatcca
     2581 gtagttcaca tactggccat catgggcaca ggtggactgc gcgatttcgt tctcgtggtg
     2641 cgggaacatc aggtctgaac cgccgccgtg gatatcaaag tggttaccca gctgcttgca
     2701 gttcattgcc gaacattcaa tgtgccagcc tggacggccc gcgccccacg gagacggcca
     2761 gctcggttcg ccctctttcg acatcttcca cagaacgaag tccatcgggt tgcgtttgtc
     2821 gtcgaccacg tcaacgcgcg cacctgcctg cagctggtcg agatcctgac gcgacagcac
     2881 gccatatgtc ggatcggtcg ggacgtcgaa catcacgtcg ccgttgtccg ccacataagc
     2941 gtgacctttg gcgatcagtt gttcagtgag ttcaataatt tctgcgatat ggtgcgtcgc
     3001 gcgcggctcc atatccgggc gcagaatgtt caaagcatca aaatctttgt gcatttcggc
     3061 gatcatgcga tccaccagcg ccacaaagct ttcgccattt tcattggcgc gtttgatgat
     3121 tttgtcgtcg atatcggtaa tgttgcgcac atacttcagt ttatagccga ggaaacgcag
     3181 atagcgcgca accacgtcaa aagcaacaaa ggtacgcccg tgaccgatat gacagagatc
     3241 gtaaacggtg attccacaca catacatgcc gacttccccg gcgtgaatag gcttaaattc
     3301 ctctttttgg cgtgtcagag tattgaagat ttttagcatc gaagattccg tttagacatg
     3361 tgtgggtaat tgagttgcgt ataataccca tatttcccgc gcgaatcagc atacattgcg
     3421 tgatgatccg atcgtgcggt tatgctataa caccacccta tatatgaccc gaactgggtt
     3481 gaagcaccaa tcaaacggaa caggatgcaa aaatggttac tttccacacc aatcacggcg
     3541 atattgtcat caaaactttt gacgataaag cacctgaaac agttaaaaac ttcctggact
     3601 actgccgcga aggtttttac aacaacacca ttttccaccg tgttatcaac ggctttatga
     3661 ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc caccaaagaa ccgatcaaaa
     3721 acgaagccaa caacggtctg aaaaataccc gtggtacgct ggcaatggcc cgtactcagg
     3781 ctccgcactc tgcaactgca cagttcttca tcaacgtggt tgataacgac ttcctgaact
     3841 tctctggcga aagcctgcaa ggttggggct actgcgtgtt tgctgaagtg gttgaaggca
     3901 tggacgtggt agacaaaatc aaaggtgttg caaccggtcg tagcggtatg caccaggacg
     3961 tgccaaaaga agacgttatc attgaaagcg tgaccgttag cgagtaatcg tggcgacact
     4021 ctttattgca gatcttcatc tctgcgtgga agaaccggcg atcaccgccg gttttctgcg
     4081 ttttttagcg ggggaagccc gcaaggccga agcgctgtat attcttggcg atctgtttga
     4141 agcatggatt ggcgacgacg atcccaaccc actccatcgc cagatggcgg cagcgatcaa
     4201 agcggtgtcc gattccggcg ttccctgtta tttcattcat ggcaaccgtg attttctgct
     4261 cggcaaacgc tttgcccgtg aaagcggcat gacattattg ccggaagaaa aggtgctcga
     4321 actttatggt cgccgggtgt tgattatgca tggcgacacg ctgtgcaccg atgacgcggg
     4381 ttatcaggct tttcgcgcca aagtccacaa accctggctg caaatgctat tcctcgccct
     4441 gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcga gcgaacagta aagaagccaa
     4501 cagcagtaaa tcgctggcga tcatggacgt taaccaaaac gcggtagtca gtgcgatgga
     4561 aaaacatcag gtgcaatggc tgatccacgg acatacccat cgcccggcgg tacatgaact
     4621 tatcgccaac cagcaacctg cttttcgcgt ggtactcggt gcctggcata cggaaggttc
     4681 gatggtgaaa gtcacggcgg atgacgttga gctgattcat tttccgtttt aaaaaacccg
     4741 caactttgct gatttcacag ccacgcaacc gttttccttg ctctctttcc gtgctattct
     4801 ctgtgccctc taaagccgag agttgtgcac cacaggagtt ttaagacgca tgtcttcccg
     4861 caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa agcgactggg ctaccatgca
     4921 gttcgccgcc gaaatcttcg aaatcctgaa tgtcccgcac cacgttgaag tggtttctgc
     4981 tcaccgcacc cccgataagc tgttcagctt cgccgaaagc gccgaagaga acggttatca
     5041 ggtgattatt gcgggcgcag gcggcgcagc gcacctgcct ggtatgattg ccgccaaaac
     5101 gctggtgcca gtgctgggcg tgccagtaca gagcgccgca ctgagcggtg tcgatagcct
     5161 ctactccatc gtacaaatgc cgcgcggcat tccggtgggt acgctggcga ttggtaaagc
     5221 tggcgcggca aacgcggcgt tactggcagc acaaattctt gcgactcatg ataaagaact
     5281 gcaccagcgt ctgaatgact ggcgcaaagc ccagaccgac gaagtgctgg aaaacccgga
     5341 cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta acgggcagtt aggccgtatg
     5401 ctgcgccagg caggtgaacc gttaggcatt gctgtctggc cggtcgggct ggacgctgaa
     5461 ccggcggcgg tgccttttca acaaagcgtg attaccgctg agatcgaacg ctggccggaa
     5521 accgcattaa cccgcgagct ggcgcgtcat ccggcctttg tgaaccgcga tgtgttcccg
     5581 attattgccg accgtctgac tcagaagcag cttttcgata agctccacct gccgaccgca
     5641 ccgtggcagt tacttgccga tcgcagcgag tggcctgcgg tgtttgagcg tttaggtgaa
     5701 ctggcgattg ttaagcgtcg cactggtggc tatgacggtc gcggtcaatg gcgtttacgt
     5761 gccaatgaaa ccgaacagtt accggcagag tgttacggcg aatgtattgt cgagcagggc
     5821 attaacttct ctggtgaagt gtcgctggtt ggcgcacgcg gatttgatgg cagcaccgtg
     5881 ttttatccgc tgacgcataa cctgcatcag gacggtattt tgcgcaccag cgtcgctttt
     5941 ccgcaggcca atgcgcagca gcaagcgcaa gccgaagaga tgctgtcggc gattatgcag
     6001 gagctgggct atgtgggcgt gatggcgatg gagtgttttg tcaccccgca aggtctgctg
     6061 atcaacgaac tggcaccgcg tgtgcataac agcggtcact ggacacaaaa cggtgccagc
     6121 atcagccagt ttgagctgca tctgcgggcg attaccgatc tgccgttacc gcaaccggta
     6181 gtgaatagtc cgtcggtgat gatcaacctg attggtagcg atgtgaatta tgactggctg
     6241 aaactgccgc tggtgcatct gcactggtac gacaaagaag tccgtccggg gcgtaaagtg
     6301 gggcatctga atttgaccga cagcgacaca tcgcgtctga ccgcgacgct ggaagccttg
     6361 atcccgctgc tgccgccgga gtatgccagc ggcgtgatgt gggcgcagag taagttcagt
     6421 taagcaggtg ctcatttgat tgccggatgc ggcgtaaacg ccttatccgg cttaccgctc
     6481 cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat ggtgcaccga
     6541 ttgccggatg ctctcatcac tacagcgaaa tacaggtccc cgcttcgccc gccagcgtct
     6601 cttcaattcg cgataacgcc ccaatccacg cgggtttacc acggcttctg acataaccac
     6661 ttaccgccgt taccttcggc cccatcgaac cataggcttt ggcaaatggc gctaactcat
     6721 ccggtgtggc atggcgaatg gcgcgttgct gcggcgttcc ccagttttca taaaccgcgt
     6781 cagcatcggt gaggatcacc agtccatcgg cattaatctg ctcggcgagc aacgcagcgg
     6841 cgagatcttt atcaatcacc gcttcactcc ctgccccatc ttccgtcaca ggcacaccgc
     6901 cgccgccact gcaaatcacc acatgcccct ctttgagcaa caactcgatg gcttcgctgt
     6961 cgagaatttt acgcggttgc ggagacgcca ccacccggcg caaatattta ccatcacgtt
     7021 tcatctgcca gccgtaagcc gcttccagtg ccttttgttc ttctggctga taaaccggac
     7081 caataaattt ctctggctgc aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca
     7141 gcaccgtcgt gacaggcggc atctgcggct gtgcgctcaa actctgcgcc agcatatagc
     7201 caatcatccc ctggctttcc gccaccagca catccagcgg atacggatct acctctttcc
     7261 acgccagatt ctgcaatgcc agcagcccca cctgcggccc attgccgtga acaatcgcca
     7321 gccgataaga acgggccagg cgcgccagcg cgggtacagc actggcgata ttgcgatatt
     7381 gattttctgc cgtcagcgcc tcaccgcgct ggagtaaggc gttgccccca agagctacaa
     7441 ccagtgtttt catggtgttc cttgtaataa ttgttgacca agccagaacc ccagcagcgt
     7501 atctgcccct gacgtgtgcc ccagcgccag cagcgaatca atcgcaacag cggtacgttt
     7561 cgggcaactc agagcatgaa caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc
     7621 gtagcgtaaa taactgacgc tcaccgctgt ggtaacgagt tgaagattgt cggagcaggc
     7681 aaaaaactgg cgaccggagc gcgcatctaa agcgccataa taccaggccg ccagcagcat
     7741 tccgctcaac gtgtcatcat gacttggcgt taatccgggg cctttaccca gccagtgccg
     7801 ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag tgacgaaact ggcgtaattc
     7861 agcggtcagc ggatcgcttg ccgccatcgc cagtggtccg aaaagtccgg tttcctccgc
     7921 gcgttgcatc catgcagctg caagcggttg aggatgtgaa ggcggcgtaa tacgcagcaa
     7981 acaataacgc tgtggctgtt taaccgtgaa acgcccgagg cgaatccctt gagccacaac
     8041 ctgtggtcgt tcattgccgc ataatccacc gcataacgca tcgaattgcg cgcggcgaag
     8101 cacccatcct ccggggccga aaccactacc ctgacgatgc agcgttaaca gttcgccgct
     8161 ttccgtcatc aggttaatcg cccgtcgcca cactcccgct aaccgccagg actggcgata
     8221 attcggtgca ctacggctgg caagcagagg atggatgatc gtcattgcgc gttcctcaac
     8281 caatccccat gctttcagcc agtgcttcca gcgcctgttc aaaacacgcc agcggtgccc
     8341 gcacagtgcc tgcgccaatc tgcccgatcc ccgcctcttt atgggcgata ccggtattga
     8401 tgagtggcgt aatgccggtt tctaccacgc gacgaatgtc cagtcccagg cacgcgccct
     8461 gaaaatccca gcctgggatc tgcaactgca tattgcgctc aaggtaaatt tccgccatct
     8521 cttcagatac cgctctggcc gcttccatgc cacccgcacc gacaaagcgc gttacgccag
     8581 gcgctgcgat cattgccgca cctccgatac caaaggtttc ggtaatcgcg ctatcgccca
     8641 tatccgggtt cgcctgctcc tgcgagaagc cggtgaaaaa cagaccttgc ggagtgttta
     8701 caggcgcagt aaaccagcgt tcgcccagcc cgcttacccg aatcccgaac atattgccgt
     8761 tgcgggtcat tgccgtgacg atgctgcctg cacggatcat cgcgccagca tccatcgccg
     8821 ccttgcagta agccatcgcg aggttgagga agaactgatc ggtcacgctg aggaaatcca
     8881 tcacttcggc gatgtgctgt ttatcatgat cgaggcgagc aatttgtggg gccagcgcac
     8941 gcattaacag tgcagaggaa gcaatattgc gttgatggaa ctcatcgccc atcgtaatgc
     9001 cctgcgccat catcgccgtg agatcgatac cgcgctccat gcgccccagc gccgcgctta
     9061 atactggcat taacacatcg cgcatccagc gatggcgagt caggacatct tcgccgtaag
     9121 cgccaaaacg catcactttg ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt
     9181 cggtcacgtt ctcgaccacc agcatcggca tactggcaga agtaatgccg cccattggcc
     9241 cgacggcatt cacatggtga caaggaatga agttcacttc cccctgctcc agtattgcca
     9301 gcgcctgcgc ttcatctttc gcccagcctt cgaacagaca tgcgcccacg cacgcccctt
     9361 tcatgggtcc ggtcatctcc tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc
     9421 cctcgtttag ttcgctgata agtgaagaag ccggttgcac atccagccag tgtggacgag
     9481 cgcgacgaat ttgttcgata accgcagcat tggcttgcgc cactgatgta aacatgggaa
     9541 ccccttattg caaacgttct aataaacgag ccagtttttt attgccgccg gcgactggcg
     9601 accattggta atgcacaacc ggtttgctgg cgctttgtag ctccagcgca aagctgcgta
     9661 atccgatgtt aatcacggcg acgttttcca gtaatgacgg tgtgtgttgc tgagtggcag
     9721 gcgaaagcgg acgaatcagt gccgccgcca gcaaggtggc ttccggcagc gaactcacta
     9781 ccgcgatccc tgcatcttcc agcgtggcga tttgctgcga gcggcattgc gggtcacgtt
     9841 cagtgcctgt caccgtggca atggcataca gtggttgatt gtctgaacgc gcggcacagg
     9901 ctttttgcca ggcgcttacc agcgaggcgg caggatcggc ggtcgcaccg aagccaatca
     9961 cgacatcaag cagcaatacg cgcacttgcg gtttagcgcc gagatcggca attaactgat
    10021 tgcgtaaggc tgggtcgatc atcggatggg gacgcccgac ggtgtagaaa tcatcgccga
    10081 ggtcaataat ctggtgacca tcggcgtcga gcatcatgcc gtgatgatgg gtatcgtcgg
    10141 cttccacgcc aaggtgcccg gcaagtaatc ccgccgcttc ggcagccagc gtaccgccgg
    10201 tatacaaacc gcaaataaat ccgctgctgg caggcgttat cgcgttacgt cgcgccgtaa
    10261 cgcgtgaaag caggcaagcc aggcgtgcgg cctcatccag cgaggaggca aaccagacat
    10321 tctcgtcgca ggccaccgcc ggggtataac ctaaaaacag cgccaccgtc ggtttgccgg
    10381 ttgctttcat ggcattaaca attttcagac gcacagcttc ggcaggcggt tttgaaacaa
    10441 atgccagcat ttcgcttttc tcgtctgcac tgagcatttc cagcgccgtc agcgcactga
    10501 tgccgcccac ttcacggctg aggtcgcgcc cgccaaggcc aatcgcgtga gtaattccct
    10561 cccctgccag cgcaatctgt gagcacagct cctgaatccc ggtaccggaa gcgccaatga
    10621 cgccaatgtt gccttccggc atcacgttag caaaagccag cggcgtgccg gcaatcatcg
    10681 acgtaccgca gtccggcccc atcaccagca agcctttttc tcgcgcgcgg gttttaagtt
    10741 ggatttcatc ttccagcgtg acgttatcgg agaacatcat cacgttgagg ttgcgatcca
    10801 gcgcctgatt cgccagctcc gccgcatact cgccagccac tgaaatcagc gccagactgg
    10861 catcggataa tttctgacag gcactgtccc agcgacgcac ctgcgtcaag gactggctgc
    10921 tgcctgaccc ctgcgccagt tgttttagcg cctcttcaag ctgctgcata atcgcctgcg
    10981 cgatccccgc atccgccgct tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt
    11041 tattaaaatc gtcatgccag aaacctgtgg tatctaataa cgctttattg gcgggcgtac
    11101 ccatcattac ggaaacatca tcaacatttt ctgattcgct gagttttcgt gaaataatca
    11161 ttaaactgac cgaatcctga aaacaccctt ttttaataaa ggcgtggatc ataacaactc
    11221 cttaaaaatt gcacccatgg caattgcacg agcagtgtta atgtggcgat tacggtatcg
    11281 atgacaacgg gtaaataagg tgatgaatat cacacaggga ttattaacaa aagaattcaa
    11341 ataaccgcca tgctattacg tgttattttt taataagcgt ggttgtttta aaaaccaggt
    11401 taaaagcgag cggattatca aataaattct aactatattt ttttgcctgt ctggatcaca
    11461 taatccagat attttccctg tatgttaatt gcagtcatgc ttcacaccgt cgttaaaaag
    11521 gaagacagat gaaaatcagt cgggaaacac tccaccagct aattgagaat aaactctgcc
    11581 aggctgggtt aaaacgtgag cacgctgcaa ccgtggctga agtattggtt tacgccgatg
    11641 ccagagggat ccactctcat ggcgcggtgc gcgtggaata ctacgcggaa cgcatttcaa
    11701 aaggcggcac caaccgcgaa ccggagtttc gtcttgagga aaccgggcca tgctcggcaa
    11761 ttttacatgc cgacaatgcc gccggacagg tcgcggcgaa aatgggtatg gaacatgcca
    11821 tcaaaaccgc ccagcaaaat ggcgttgcgg tggtcggtat cagccggatg ggtcacagcg
    11881 gcgcaatctc ttattttgtg cagcaggcag cccgcgccgg attcattggc atttcgatgt
    11941 gccagtccga tccaatggtg gtgccgtttg gcggcgcgga aatttactac ggtactaacc
    12001 ccctggcctt tgccgcgccg ggagaaggcg acgagatcct tacctttgat atggcgacta
    12061 ccgtacaggc atggggaaaa gtgctcgacg cccgctcgcg taatatgtct atcccggata
    12121 cctgggcggt cgataaaaac ggtgcaccaa caaccgatcc gttcgcggta catgctctgc
    12181 tccccgccgc cgggccaaaa gggtatggcc tgatgatgat gattgacgtc ctctcaggcg
    12241 tcttactcgg cttaccgttc gggcgacagg ttagttcgat gtatgacgat ttacacgcag
    12301 ggcgtaattt ggggcaatta catatagtta ttaatccgaa ctttttctcc tccagcgaat
    12361 tattccgtca acatcttagc cagaccatgc gcgaattaaa tgccattacc cccgcgcccg
    12421 gttttaacca agtttattat cccggacagg atcaggatat taaacaacgc aaagccgccg
    12481 tcgaaggcat cgaaattgtt gatgatattt accagtattt aatttccgac gcgctttata
    12541 acacgtcata cgaaacgaaa aatccctttg cgcaataatt attgagacag gactttctta
    12601 tgattacaca ttttcgtcaa gccatagaag aaacgctgcc ctggctttcc tcttttggcg
    12661 ctgacccaac gggtgggatg acccgtttac tttattcgcc ggaatggctg gaaacccagc
    12721 agcaatttaa aaaaagaatg gcagcaagtg ggctggaaac acgtttcgat gaagtaggga
    12781 atttatacgg tcgcctgaat ggtaccgaat atccacagga agtgattctg agcggttcgc
    12841 atatcgatac cgtggttaac ggcggtaacc tcgacgggca attcggcgcg ctggcggcgt
    12901 ggctggcaat tgactggttg aaaacgcaat acggcgcgcc gctacgtacg gtcgaagtgg
    12961 tggcgatggc agaagaagaa ggcagccgct tcccgtatgt cttctggggc agtaaaaata
    13021 tctttgggct ggcgaatcct gacgacgtgc ggaatatctg tgatgccaaa ggaaatagtt
    13081 ttgtcgatgc aatgaaggct tgcggattta ctcttccgaa cgccccgcta actccgcgtc
    13141 aggatattaa agcctttgtc gaactgcata tcgaacaggg ctgtgtgctg gaaagtaatg
    13201 ggcagtcaat tggcgtggtg aatgcaattg tcgggcaacg tcgctatacg gtgacgctga
    13261 acggcgaatc aaaccatgca ggcaccacgc cgatgggtta tcgtcgtgat acggtttacg
    13321 ctttcagtcg catttgccat cagtcgatcg aaaaagcgaa aaagatgggc gatccgctgg
    13381 ttctgacctt tggcaaagta gagccgcgcc cgaatacggt aaatgtggtg ccgggtaaaa
    13441 ccacgttcac cattgattgt cgtcataccg acgctgccgt gctgcgtgat ttcacccaac
    13501 agttagaaaa cgacatgcgg gcgatttgcg atgaaatgga catttgtatt gatatcgatt
    13561 tatggatgga cgaagaaccc gtgccgatga ataaggacct ggtcgccacc ctgacagaat
    13621 tgtgtgaaag tgaaaaactg aattactggg tgatgcacag tggtgccggg cacgacgcgc
    13681 aaattttcgc gcctcgcgta ccaacctgca tgatttttat ccccagcatc aacgggatca
    13741 gccataaccc ggcggaacgc accaatatta ccgaccttgc cgaaggggtc aaaacgttgg
    13801 cactcatgct ttatcaactt gcctggcaga aataaggagt cataaatggg atatttaaat
    13861 aacgtcaccg gttaccgcga tgatttactg gctaaccgtg cgattgttaa acacggtaat
    13921 ttcgcactgt taaccccgga tggtctggta aaaaatatta ttccgggctt tgaaaattgt
    13981 gacgcgacaa tcctctccac gccaaagctg ggtgcctctt ttgttgatta tctggtcaca
    14041 ctgcatcaaa acggtggcaa ccaacagggc ttcggtggcg aaggcattga aacgttcctg
    14101 tatgtgatct ctggaaatat cactgccaaa gccgaaggca aaacatttgc cttaagcgaa
    14161 ggtggctatc tttattgccc accaggctcc ttaatgacgt ttgttaacgc ccaggccgaa
    14221 gacagccaaa tctttttata taagcgccgc tatgttccgg tagaaggcca tgcaccgtgg
    14281 ctggtttctg gcaatgccag cgaactggaa cgcattcact atgaaggtat ggatgatgtg
    14341 atcctgctgg attttctgcc caaagagtta ggttttgata tgaacatgca tatcctctct
    14401 tttgcaccag gtgccagcca cggttatatc gaaacacatg ttcaggaaca cggtgcctat
    14461 attctttccg gtcagggggt ttataacctc gacaataact ggatcccggt gaaaaaaggc
    14521 gattacatct ttatgggcgc ttattcttta caggctggtt atggcgtagg gcgtggtgaa
    14581 gcgttcagct atatttactc gaaagattgt aaccgcgacg tcgagattta agtcatacca
    14641 ctcgcctgat tatttttagc tatgttggat tttgccacgg tttataccgt ggcttttttt
    14701 gtagtaaatt caggtaaaaa aagatgcgat ataaatcgca tccgctttaa aggtaagggt
    14761 cagtttttaa ttccctgacc tattttaatg gcgcaggcaa tatttcgtgc gctgttgaag
    14821 agattggttt caccgctggc gagcacttcg gctaaaggtg ccagacgtgg caaaatgctg
    14881 aataccgcat caatgccgta ctggtgcacc acttccacgc catcacccaa tacgccagca
    14941 atcccaatca ccgggacatt aaactgcttc gccaccgacg ccacacccag cggcgcttta
    15001 ccgcctgccg tttgcgagtc gatgcgccct tccccggtaa tcaccagtgc tgcgccctgc
    15061 actgcctgcg caagattgac cgcattcaac acaatctcaa tacctggttt aatatccgca
    15121 ttgagaaata ccgccgccgc aatacccata ccgcccgcag cgccgccgcc cgccatctgg
    15181 cagacgttga tttcagtttg ctgttgtaaa acacgggcgt aattttgcag cccctgttcc
    15241 agctcttcga ccatttccgg cgttgcccct ttttgcgggc caaacaccgc cgccgcgccg
    15301 cgtgccccta ccagcgggtt atcaacgtca catgctactt caatatggca gttcgccaga
    15361 cgcggatcgc attcctccat ctcaatgctt gccacgcgcg ctaaattgcc gccgttagct
    15421 gccagcacct gaccgtcggc atcaaggaaa cgcacaccga gcgcctgcgc catgcccata
    15481 ccaccgtcga ccgtcgcact gccgccaatg ccgagaataa tatggcgaat gccgttatcc
    15541 agcgcatgac gaattaactc ccccgtacca aaactggagg ccagcaacgg attacgcttt
    15601 tcaggcgcga ccagcatcag gccacttgct gccgccatct caatcaccgc cgttttcccg
    15661 tcgccggtaa ggccataaaa agcattcact ttttcgccca tcggcccgca gacttcaagc
    15721 gtcacgatgt tgccgcccgt cgcggcgacc atcgcatcca ccgttccttc gccgccatcc
    15781 gctatcggca aacagatata gttcgcatcg ggaaagaggg tcgaaaaccc ggctttaatt
    15841 gcctgacaac atttttctgc acttaagctc tctttaaaag agtctggcgc aatgacaatc
    15901 ttcattatca atcctttttc aacaaattat tcgatacgtt cccacggaat aaggttatcc
    15961 ataagaacag ccagtaaaat cccgaccaat aagccgttac tgagcagagg acgaagcgtt
    16021 aatggaaggt cttgcagata cacaggcggt aatgccatta aaaatatgcc gacaaataac
    16081 ggcaatgcga gtcgataaat attgcgagcg gtaaacgtta tttgctggct aaacaccagc
    16141 gcggaaaaga gtaaaggcag ataagaaacc agcatgaccg cgctactcac gggtaaaggg
    16201 atactgcaaa acagtcgcgt gagtgccgga accagcgcca ccagcaggca aataacgctg
    16261 ccataaataa acgaacgccg cgtgtaatcg cctgtctggg ttaataaacc aatggatgaa
    16321 acaaacggtg aaaatggaat taccgccagc ggtacggtta tcagcgtcat aaatccggtc
    16381 gccacaaagc tgcgacgata acgcgtattt cccgcgccct gctgcggata aaaaacatcc
    16441 gtgccccgaa tcgcaccgta ggtattgctg atattcacca gacctgtaat caccgccgtc
    16501 agaataattc ccggagacaa agcacctcca ctgccgagcg ggaaccactg ccagtgcaac
    16561 tcaccggaga gcgagtgcga agaaggaaag caaaagtacc acaacaacca gccggttatg
    16621 gtgccgacca gcaggccata acgggcaaaa cgttgcggca ggaagataat catccccagt
    16681 accaggcaca tcaccgccac cgagagcgcg aacggcggta actgaatttt aaaatttggg
    16741 tcggctatgc caaacggcag cccgagcata cctttgaaaa agatagtggt cagctgcgcg
    16801 cccagcatca acataaacaa gaccatcacc gacggcgtaa ataaccgtgc caggcgatgg
    16861 cctaatccgc taaaaccaat caacatcgtc agcacgccgg agagcgcaat ccccaccgcc
    16921 aggctggtag cgatatcgtt gatcggtgtc ccgcgcgatg cttcgccaag ggtgatagta
    16981 aggatggttc cccaccacag gccacccggc ccttccataa tagcgcgacg atgaccgcag
    17041 aacgcctgag cgaagcaggc cagtgcggta gcaagaaaag catattgcgt gagcgtaagc
    17101 aggctacttt gcggcaactg aaaagcagaa agtagcgtag gaggaaccac aaccgtgttg
    17161 caaaaaataa agaaaaacca ctgaaatcct gataacaggc tttcgcggct cactgcaaaa
    17221 ttgaacataa acacacctta accggagaat gcccgccgca aggacgggca ttgcaggggc
    17281 cagattactg ctgatgttta aggataaatt gacctttcgg cgcaacaggg aagccctgtt
    17341 caatgtcgta aatcacatca ccacgtaaga tggttttcgt gatacgcgcg ccaatggtac
    17401 ggccaacata cgggctgact ttgtggcgat attccagatc gtcattggta agaacatagc
    17461 tgctattcgg ctgaatgaag acgaagtcgg catcttttcc tggggcgata cggccttttt
    17521 gctgcagacc gaaaatatct gctgcgttag tcgccattaa tttgccgaac attggcagag
    17581 acattccgcg tttctgtacc gcttcatcga acatcacgtc catacagttt tgcagaccgg
    17641 caataccgcc ccatgcttcc atgatgttgc cggctttcat ttccggaggg catggtgagt
    17701 ggtcggaaac caggcagtcg atttcaccgt taaacagttt ttcccacatg cctttctggt
    17761 tttccagatc gcggatcggc ggtgaacact tcgccagagt gccgatttct tcgaactgat
    17821 cggtatccag tacaaagtaa tgcgggcagg attcacaagt aacgtcctga ccttcctgac
    17881 gtgcacgagt cacttcttca acgccttccg ggctgctgat atggcaaacg tgcagacggc
    17941 aaccggcaac tttcgccaga tacagcacgc ggcgaatcgc ttccacttcg gtaaataccg
    18001 gacgcgaagc cacatagtca tgggcagtta cgcgaccttc acgtttcgct tcttcgccca
    18061 gtgcgtcaca gatcagcgcg ttttcgcagt gcaccagcac cggctgcccc agttcgccca
    18121 gcttctgcgc acctttgaag aactgccagt cgtttacatc acggaagtcg ttgtcgatac
    18181 cgcgatcgcc acaggtcgca acgaagcatt tgaagccgac aacgcccact tcatccagct
    18241 catgcagacg gtcgatgttg taagacacca ggccaccgag ttgtgccgca tcgatagtca
    18301 gcttgccttt agcggcatcg aacttcagtt caattgaagc gcggtcaacc gttgcaggca
    18361 gctggttgag cggcatttcg atcatggtgg tgataccacc ttttgccgct gcgcgagtac
    18421 cggtttcata accttcccag tggctacgac ccggttcaga aatatgggtg tgcgcatcaa
    18481 ccatgcccgg cgaaaccacc agaccagacg catccataac ttcttttgca tcgcccagat
    18541 cctgaccgat agcagcaatt ttcccgcctt taacggcgat atctacaacg cgagcttcgt
    18601 tttctaaaat aacggtgccg tttttaatga ttaaatcaaa agacataaca aactccttgt
    18661 aaaatacaga ttggttttta actcaatgtt caatatcggg attaattaac ctatggtttt
    18721 ttgctctcct gttttttctg ctgttgtacg tttctttaat aaggcgtagg ccgcaaaggc
    18781 gacgatgacg ccgacaaacc atgaaacacg cgataacggt tccataaagg gaataaactt
    18841 accgccaaga gataaaataa cggccaccag agttactgaa aacgcggtga ggttaaaacc
    18901 gttatcgtaa tatttataat cgccaggtgc ggtgtacagt tcatcaagat taatttgccc
    18961 acgcatcacc acaaaataat gtgccatcat gacaccaatt accggaccca gcattccgcc
    19021 gatgatatcg aggaacagat aaatactgtc ctgattttcc attaatttcc acgggcagat
    19081 cagcaggctg ataatactgg caatcagtac gccgttttta taggtcagtt ttgtcggtgc
    19141 aatggcagca atctgataac cggccggaat aatattaccg gtggcgttag tggaaatagt
    19201 cgtcatcaga ataaccagta ccgcaaagaa cgaggcaaac aggctgtccc aacgctgaac
    19261 aatatccagc acgttccagg tgtcagcgcc gtagtgaata ctggctccgg caataataca
    19321 taccccagcg accgcaaaca gaatataggc cacaactaaa cccagcgttt gccccagcgc
    19381 ttgctcacga aacgagtggg cgttttgcgt aaagtcggat gcgctcaccg ccggtgccgc
    19441 ccagaccgca actaccgcgt taatcaccac caggaacaag aatccactgt tttctgcttt
    19501 ctgaataccg ctcggaatgt agtcaaagat tggaccgagc ccgaccagcg aaatcgccca
    19561 aatcgccata ccgccgaaaa cgatatagat gcacgggtta agaatggcag tgaatttatt
    19621 taaaactttg ccaccgccaa aacctatacc aacgttgacc agccagaaga gtaagaaagt
    19681 aattaagccc ggtagagaaa gccctaacag cgtgaaatca ccaccgagag ttaaaaatcc
    19741 cggccagatt ttgccaatca gaatcaagca ggccagtgac cccgcataac attgcaggca
    19801 aaaccacata atggcagcaa taccgcctct taataatccg ggaaacagtg cgccacgtac
    19861 gccataagaa gcacgcagga tcatggcaaa cggtacgccg tatttactgc ccgcagcgcc
    19921 gtttaatacc attaccgcgg caatgaaaaa ggcgctgagg ataattgcca gcataatact
    19981 aaaggttgac aagccgagaa taaaaaagcc gccgaccatc acataattgg gaacgttatg
    20041 aaccgaaccc atccataagg taaaatagtt aaatgttttc caggttcgct ggctttgcgt
    20101 tttcggcaat agatcttcgc tatagccgcg ttgctggaat agttttctct gatgttccat
    20161 aaatccctct taatttcacg tatcgaataa cgttgtttta atctagctat gaggaacaaa
    20221 taaaataaaa atattatttt taatgctgaa gcaacatctt tggctgcttt ctttttttaa
    20281 tattgctggc cgattcattt tctgatatcg gccattttta ttgcgggtat caggccagtt
    20341 tatggttagc cattaattcc agcgcctgca ctaacgcaga gtgatccaac tggctgccac
    20401 cgtttgccgc gcaggtatta aataactcct ggcaggtcgc agtgtttggc aggttcagcg
    20461 ctagcgcttt cgcactttgc agtgccaggt tgagatcttt ctggtgcagc gcaattttga
    20521 aacctggatt aaaagtgcgt ttaatcatgc gctcaccgtg aacttccaga atacgtgagg
    20581 aagcaaagcc gcccatcagc gcctggcgca cacgtaccgg gtccgcaccg gcttttgaag
    20641 caaatagcag ggcttcagaa accgcttcaa tattgagcgc cacaataatc tgatttgcca
    20701 ctttgcaggt ttgaccatcg ccgttaccgc ccacgagggt gatattttta ccgagcagtt
    20761 caaacagcgg tttaacacgt tcaaataccg cttcatcacc gccaaccata atcgacaacg
    20821 tcccttcacg cgcaccgatt tcaccgccgg agactggcgc atcgagataa tcgccgccca
    20881 gttcattcac ctgacgagcg aaacgcttag tttcaatcgg ggaaatggag ctcatatcaa
    20941 caatggtttt gcccttcagc gaggctttgg tacaaccatt ttcaccgaac agaacttctt
    21001 caacctgtgg tgtatctggc accataataa aaatgatgtc cgatgcttcc gttacctggc
    21061 gcgcagtttc aacactgacg gcacccagtg acagtaattc atcagcaacc ggaccaatgg
    21121 ttgtgacatg cagttgatga ccggcacgcg ccagattaat ggccatcggt gtacccataa
    21181 tgcctaagcc aataaatccc agtttcataa ttaacctctt ttaaatttcg cttttcctga
    21241 attcagacaa cacgatcccc tgccaactta gcggcctaaa aaagcattgc ctgaacagcg
    21301 ttacgtttta acggtacgga tccatccagc gtaaaccggc ttccgtggtg gtttggggtg
    21361 tatattcaca cccaacccaa ccgttgtagt cagaattttc gattacctta aagagataat
    21421 cataattaat ttctccggtc cccggttcgc cgcgatgcgg attatcagca atttgcaggt
    21481 gaccaatttt atcggcccac tgagtcatgg tgttggttaa ttcaccttcc atccgctgca
    21541 tgtgataaat gtcatactga attttcaaat tgcagcaacc aacatcatca atcaatttca
    21601 gcgcctgccg agttccggtg agatggaaac caggaatatc aaaatggtta ataggttcaa
    21661 tcagtaataa aatatcttct ttcatcagca tattcgcggc ataacgcagg ttttctacaa
    21721 gcgttgcgtg aatctgttca ctgctgaaac cagccggcgt tttaccgacc agacagttaa
    21781 tttttttatt acccagcgca cgggcataac gaatcgctgc tgctacgcca tcccgaaact
    21841 cttcttcacg gccaggaata caggcaatac cgcgctcccc cgccgcccag tcacctgccg
    21901 gtaaattgtg cagcgtatgt tcgagtttat tactcgccag cacctgtttt aattcttcaa
    21961 tgtcgtagtc ataaggaaac ataaattcaa cgccgcgaaa accacactgc gcagctttct
    22021 caaaacgggc gagaaaatca tattcaccaa ataacatcga taaattagca gagaaacgta
    22081 acattattta tctcccttat tcatagtgca tgaagcaggt ttcagtcggt gcatccgctg
    22141 cgttatcggc gatatcttca aattccatga cgttatccag ttcgctgccc atcgaaatat
    22201 tggtcacacg ctcgagaata acttccacga ctaccggtac ccgatattgc gccattaagg
    22261 ctttcgcctg ttcaaaggct ggcgcaatat cttccggttt gaagacgcga atagctttac
    22321 aacctaaacc ttccgctact tttacgtggt caacaccgta gccattcact tcactggagt
    22381 tgatattctc gaaagcgagt tgcacgcagt agtccatatc aaacgcgcgc tgcgactggc
    22441 gaatcagccc cagataagcg ttattgacca gtacatggat gtacggaatg ttgaactgtg
    22501 cgccaacggc caattcttca atcaggaact ggaagtcgaa gtcaccggaa atcgccacca
    22561 ctttgcgctc tggatcagcg gcacaaaccc ccagcgccgc cggaatggtc cagcccagcg
    22621 ggcctgcctg cccacagttg atccagtggc ggtctttaaa gacatgcagc atttgcgcag
    22681 cggcaatttg cgacagacca atggtggtga catagcaaac atcacgtcca aaagctttgt
    22741 tcatctcttc atacacacgc tgcggtttaa ccggaacgtt gtcgaagtgt gttttacgca
    22801 acaaagtacg tttgcgctgc tggcactcag caacccactc tttacggcat ggcagacgcc
    22861 ctgctttttg catttcctgc gccacttcaa ccagcagtgt cagcgccgct ttagcatcag
    22921 agacaatccc cagatccgga cacagcacgc ggccaatttg cgtcggctca atatcgatat
    22981 gaacgatttt gcgcccttcg gtgtatttct ctaccgaacc ggtatgacgg ttagcaaaac
    23041 ggttaccgat accaaacacc atgtcggacg ccagcaacgt tgcgttaccg taacgatgcg
    23101 cggtttgcag acccaccatc ccggccatca gttcatgatc gtccgggata cagccccagc
    23161 ccatcagcgt tgggatcacc ggaacgctgg tcagttcagc aaactgttgt aacagtgcag
    23221 ctgcgtcagc attaattacc ccgcccccgg caacaatcac cggacgtttg gcctggatta
    23281 acatttctac agctttttcg atctgcatac ggctggcagc aggtttgtag accggcagcg
    23341 gttcgtacat atcaggatca aactcgattt ccgcaacctg aacgtcgaac ggtaaatcca
    23401 ccagtaccgg tcccggacga ccagaacgca tcaggtgaaa tgcctgttgc agcacacgag
    23461 gcaccagcgc cgcttcacgt actgtaaccg ccattttgct gaccggttta gcaattgctt
    23521 caatatctac ggcctgaaaa tcttctttat gcagacgggc gcgcggtgcc tggccggtaa
    23581 tgcacagaat aggaatggaa tcagcagaag cggaatagag cgcggtgatc atgtccgtgc
    23641 ccgcaggacc ggaagtcccc agacatacgc cgatattccc tgccgttgcg cgggtataac
    23701 cttccgccat gtgcgaagca ccttccacat gacgcgccag aatgtgacga ataccgccgt
    23761 gcttacgcat cgctgagtag aacggattga ttgcagctcc cggaacaccg aaggcggtgg
    23821 tgataccttc tttctccagc acatacattg ccgcgtcaac ggctctcatt tttgccattt
    23881 tattcctacc tctatttatt ggaaaatttt tccaactttc aaattttgtc aaaaaaaccc
    23941 gctccgcaga gcgggatgcg acagacatta tggatgtgct ttcagtccca acgccgtgct
    24001 gatatcgcgg gcggtgtctc tgaccagctc accctgactg acaaaacgat cttctgtcag
    24061 tcttgatgaa ggcccggaga tggagatagc ggcaacaacg ctaccgacat catcgtaaat
    24121 tgctgaagct atgcaattca gacctacaac atgctcttct ttatctacgg tatagcccag
    24181 ttcacgcgct tgttccaggt ccttcagcaa ggtgggcata tccacaagcg tagttggcgt
    24241 aaactgctgc aaaccggttt gcagaatgat gctcatcaac tcctcttccg ccagcggata
    24301 aagcaacgcc ttgcccgcac cggaagcatg cagcggcaga cgactgccca gtggcgcaca
    24361 catcctgacc attgatttac actctaactg accaattaat actgcttcat tgccgttacg
    24421 gatcgcgaca ttgaccgttt cgccggaaag taacatcagg cggcgcataa acggcccggc
    24481 gacggagagg acatcgcggt tatggatgta cgccgcaccg acgttaaaga cacctaatcc
    24541 tatatgccac cagcctaatt gactgtcctg atagacaaaa tccgctgctt gtaaaacctt
    24601 cagcaagcga aaggtcgtgg agagcggcaa atccagattg agagaaatat cgctaaccga
    24661 cgaacttccc ccactttttt ccagatattg cagaatcgca attccccgct ctaacgcctg
    24721 tgcgcccttc tgtgccacag gctccgcctg tcctggcctg ccgcgccgtc taacttccgt
    24781 catacctacg ttaactccga cctacaagtg agcataaaca atgaacaatt agtttatctt
    24841 atgcaagtcg gttgcaggcg tcacgcaaaa cagagttcct gttcaggaat actttcaaca
    24901 tcacagttgt cactgccgcc gcgatcgatg gtcagaaaat cggtgacgcg ctgccaggcg
    24961 aaaagtgggt gatgccagac gttacgatgg taattcactc cctgttcgcc gttggtgata
    25021 aacgcccgca gcgttgacag gtctggtttg tcgtcgccta acgccacgac caccacaaac
    25081 acttcacctt tcatcgggat aaaggcctga gtgcccagcg gatgacgttc gagttcgtaa
    25141 atggtcagcg gcagattcgc cggttgcgcg cggttaatgc tgataagcgt acggtcttgc
    25201 tcaagaatct caaccagcgc caaatcgtgg taacgctcca ccaggccatt gttaatatgg
    25261 aaaaaatccc gttgctgagt ttcgattacg tcgccataag cactaaaggc ttcctgactt
    25321 aacggtaata cctgaagttt catctttttg tccttgtaac ataattccgt tcaaccatcg
    25381 ccgaccggtc agcttggttt caataacagg caatctaaaa ccttaagaga acaacatcaa
    25441 tacaaaagtt ggaatatttt tccaaaacta gatttagtcc acaaattgaa cagggttatc
    25501 atgtaaaaat actgataaat cagaggataa gaaaatcacg caacttgcgg atttaaaagg
    25561 ggatacctga agatgttcga tccagaaacc ttgcggactt tcattgcggt tgctgaaaca
    25621 ggaagttttt caaaagcggc agaacgatta tgtaaaacca cggcgacgat cagttatcgc
    25681 attaaacttc tggaagagaa taccggagta gcgctgtttt tccgcacgac tcgcagcgtg
    25741 acgttgacag cggctggcga gcatctactt tcccaggcca gagactggct gagctggctg
    25801 gaaagtatgc caagcgagct gcaacaggtg aatgatggcg tggaacgcca ggtgaatatt
    25861 gtcatcaaca acctgctcta ccacccccag gccgtcgccc agttgctggc gtggctgaat
    25921 gaacgttacc cctttaccca gtttcacatc tcccgacaaa tctatatggg cgtctgggat
    25981 tcgctattat acgaaggttt ttcactggct atcggcgtca cgggaaccga ggcgctggca
    26041 aataccttta gtcttgatcc cttcggatcg gtgcaatggc gctttgtcat ggcggcggat
    26101 catccgctgg cgaacgttga agagccgcta acagaagcgc agttgcggcg ctttccggcg
    26161 gtcaatattg aagacagcgc ccgcacctta accaaacgcg tcgcctggcg attgcctggg
    26221 caaaaagaga ttattgttcc tgatatggaa acgaaaatcg ccgcccatct tgcgggcgtt
    26281 ggcattggtt ttttgccaaa atcgctttgc cagtcaatga tcgataatca acaactagtt
    26341 agccgggtaa tcccaacgat gcgccctcct tcgccattga gtctggcatg gcgcaaattt
    26401 ggcagcggca aagcggtaga agatattgtg accttgttta cccagcgcag gccggaaatc
    26461 agcggatttt tagaaatttt cggcaaccca cgcagttaaa aatcaagttt ccttgcgcgt
    26521 tggcgcgtaa aatagcgcgc aagctgattt gaggttcact tttaactatg caagagagac
    26581 acacggaaca ggactatcgt gccctgctga ttgctgatac gcccattatt gatgttcgcg
    26641 cccctatcga gtttgagcaa ggcgcaatgc ccgccgctat caatctgccg ttaatgaata
    26701 acgatgaacg cgccgccgtt ggcacctgct ataaacagca aggctcagac gcagcgctgg
    26761 cgctggggca taaactggtg gcgggtgaaa ttcgtcagca gcgcatggac gcctggcggg
    26821 cagcgtgcct gcaaaatccg caaggtattc tctgctgcgc ccgtggcggt cagcgctcac
    26881 atattgtgca aagctggttg tatgcagcgg ggattgatta tccgctggtg gaaggcggtt
    26941 ataaggcact gcgccagacc gcgattcagg cgactattga actggcacaa aaaccgatag
    27001 tgctgattgg cggttgtact ggcagcggta aaacgctgtt agtgcagcaa cagccgaacg
    27061 gtgttgatct ggaagggctg gcgcgccatc gcggttcggc gtttggtcgc acgttacaac
    27121 cacaacttag ccaggcgagt tttgaaaacc tgctggctgc cgaaatgctg aaaaccgacg
    27181 cccgtcagga tttgcgcctg tgggtgctgg aagacgaaag ccggatgatc ggttcgaatc
    27241 acctgccgga atgcctgcgc gagcgaatga ctcaggcggc gattgcggtg gtagaagatc
    27301 cgtttgagat ccgtcttgag cgcctgaacg aagagtattt cttgcgtatg catcatgatt
    27361 ttacccacgc ttatggcgac gaacagggct ggcaggaata ttgcgaatac ctgcatcacg
    27421 gactttcggc gattaagcgt cggctggggt tacagcgcta taacgaactc gctgcccagc
    27481 tggatacggc gctgacaacg caactcacca ccggcagcac cgacggtcat ctggcctggc
    27541 tggtgccgtt acttaaagaa tattacgacc caatgtatcg ctatcagctt gagaaaaaag
    27601 cggaaaaagt tgtctttcgc ggtgagtggg cggaagtggc ggaatgggtt aaggcgcggt
    27661 aatgagggac gtatttgtat tagtcgcgac ttgatctgac acatggcctt gaaaggttga
    27721 gagttaccgg ttttgatatg agtgtcgaat ccttatccaa aacaagaggt aactctcatg
    27781 cttcatactg ccaatcccgt catcaaacac aaagccggtt tgctcaatct ggctgaagaa
    27841 ctcagcaacg tatcaaaagc ctgtaaaatc atgggcgttt cgcgtgatac gttttatcgt
    27901 taccgcgaac tggtcgctga aggcggtgtg gatgcgcaga ttaatcgtag tcgccgcgct
    27961 cctaacctta agaaccgtac cgatgaggca actgaacagg ctgttgttga ttacgccgtc
    28021 gctttcccga cacatggtca gcaccgggcc agcaacgagc tgcgtaaaca gggggttttt
    28081 atctccgaca gtggtgtccg ttctgtctgg ttactccata accttgagaa tctcaaaaga
    28141 cgatactgaa caaattttat caggtgacgt tccgtaaaaa gttgtatgga gttctcgatg
    28201 cagtcggcgc gactatcagt acccccgctg aaccctggac tatcgggccg ccgtactggg
    28261 gcatgtcacc ctggcgtgcc gctggttttc cgctaatggt ttatttcctc tttctttccc
    28321 ggcaagaatt tattttaatc cggaaggatt atttattttc agatagggac tcacattaaa
    28381 cacgtaacat tttaattaat gcatggctga atatgtatta cacaatgtca gatatcgtaa
    28441 aagaccagtg caattctatg taaactctct gactgttcat tttatttgtt gtttcagggt
    28501 cggaactcac cctcaataaa caactgaata cgagtagata ttcatcgtct gagctatatg
    28561 gctttacaca atagccgaca tgtcttttct cagtcaggaa ttttactttt cctcactacg
    28621 aagttgacga cttcgatatg ggatagactc ttaattcaag caattactct gcacaagcat
    28681 caggcaatcc agctccaata tttgttataa aataatgagt aacattaatt tcaccaacaa
    28741 aaaaaaggtt tacaccatcc ccttgccctg gtatcttacc atatttacac cacacttttt
    28801 ttgtatctgg atcattcttt gatacgattt tcgccacttg ttccctggcc ctcactgtca
    28861 atgttttctt aaacctaccc ccatcatcat ttgttaggga aaaataagaa aaaaaataat
    28921 catcttctac atttgtgtat attgatatat ccaccgctac gtgcggaaaa aaatctggga
    28981 gccatgtaaa aactccatca ttttctggta ttgccatata gtttatgta
//
LOCUS       DAZFYC010000059        28282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFYC010000059 DAZFYC010000000
VERSION     DAZFYC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.059"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            174..857
                     /locus_tag="ACHKW5_002789"
     CDS             174..857
                     /locus_tag="ACHKW5_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7512149.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            901..5751
                     /gene="ehaG"
                     /locus_tag="ACHKW5_002790"
     CDS             901..5751
                     /gene="ehaG"
                     /locus_tag="ACHKW5_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO7512150.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6119..7774
                     /gene="lldP"
                     /locus_tag="ACHKW5_002791"
     CDS             6119..7774
                     /gene="lldP"
                     /locus_tag="ACHKW5_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO7512151.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            7774..8550
                     /gene="lldR"
                     /locus_tag="ACHKW5_002792"
     CDS             7774..8550
                     /gene="lldR"
                     /locus_tag="ACHKW5_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO7512152.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8547..9737
                     /gene="lldD"
                     /locus_tag="ACHKW5_002793"
     CDS             8547..9737
                     /gene="lldD"
                     /locus_tag="ACHKW5_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO7512153.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSINEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9923..10396
                     /gene="trmL"
                     /locus_tag="ACHKW5_002794"
     CDS             9923..10396
                     /gene="trmL"
                     /locus_tag="ACHKW5_002794"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO7512154.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10449..11270)
                     /gene="cysE"
                     /locus_tag="ACHKW5_002795"
     CDS             complement(10449..11270)
                     /gene="cysE"
                     /locus_tag="ACHKW5_002795"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO7512155.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11350..12369)
                     /gene="gpsA"
                     /locus_tag="ACHKW5_002796"
     CDS             complement(11350..12369)
                     /gene="gpsA"
                     /locus_tag="ACHKW5_002796"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO7512156.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12369..12836)
                     /gene="secB"
                     /locus_tag="ACHKW5_002797"
     CDS             complement(12369..12836)
                     /gene="secB"
                     /locus_tag="ACHKW5_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO7512157.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12899..13150)
                     /gene="grxC"
                     /locus_tag="ACHKW5_002798"
     CDS             complement(12899..13150)
                     /gene="grxC"
                     /locus_tag="ACHKW5_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO7512158.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13292..13723)
                     /locus_tag="ACHKW5_002799"
     CDS             complement(13292..13723)
                     /locus_tag="ACHKW5_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO7512159.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13968..15512
                     /gene="gpmM"
                     /locus_tag="ACHKW5_002800"
                     /gene_synonym="pgmI"
     CDS             13968..15512
                     /gene="gpmM"
                     /locus_tag="ACHKW5_002800"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO7512160.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15546..16805
                     /gene="envC"
                     /locus_tag="ACHKW5_002801"
     CDS             15546..16805
                     /gene="envC"
                     /locus_tag="ACHKW5_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO7512161.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16809..17768
                     /locus_tag="ACHKW5_002802"
     CDS             16809..17768
                     /locus_tag="ACHKW5_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO7512162.1"
                     /translation="MFPFRRNVLAFATLLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17755..18789)
                     /gene="waaH"
                     /locus_tag="ACHKW5_002803"
     CDS             complement(17755..18789)
                     /gene="waaH"
                     /locus_tag="ACHKW5_002803"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO7512163.1"
                     /translation="MMNSTSKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19028..20053)
                     /gene="tdh"
                     /locus_tag="ACHKW5_002804"
     CDS             complement(19028..20053)
                     /gene="tdh"
                     /locus_tag="ACHKW5_002804"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO7512164.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20063..21259)
                     /gene="kbl"
                     /locus_tag="ACHKW5_002805"
     CDS             complement(20063..21259)
                     /gene="kbl"
                     /locus_tag="ACHKW5_002805"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO7512165.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21534..22391)
                     /gene="yibB"
                     /locus_tag="ACHKW5_002806"
     CDS             complement(21534..22391)
                     /gene="yibB"
                     /locus_tag="ACHKW5_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO7512166.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            22695..23627
                     /gene="rfaD"
                     /locus_tag="ACHKW5_002807"
     CDS             22695..23627
                     /gene="rfaD"
                     /locus_tag="ACHKW5_002807"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO7512167.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            23637..24683
                     /gene="rfaF"
                     /locus_tag="ACHKW5_002808"
     CDS             23637..24683
                     /gene="rfaF"
                     /locus_tag="ACHKW5_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO7512168.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            24687..25679
                     /gene="rfaC"
                     /locus_tag="ACHKW5_002809"
     CDS             24687..25679
                     /gene="rfaC"
                     /locus_tag="ACHKW5_002809"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO7512169.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            25676..26884
                     /gene="rfaL"
                     /locus_tag="ACHKW5_002810"
     CDS             25676..26884
                     /gene="rfaL"
                     /locus_tag="ACHKW5_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO7512170.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(26920..28062)
                     /locus_tag="ACHKW5_002811"
     CDS             complement(26920..28062)
                     /locus_tag="ACHKW5_002811"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO7512171.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLEKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(28071..>28282)
                     /locus_tag="ACHKW5_002812"
     CDS             complement(28071..>28282)
                     /locus_tag="ACHKW5_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO7512172.1"
                     /translation="GITKPWHDWANYASADYFRNIYNISPWRNIPYKKAVKKHEYKEK
                     YKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         7360 a         7041 c         6961 g         6920 t
ORIGIN      
        1 tacaaacaca gacatctttt gaaacgaatg atacattttt atgatgaatg cttaaaacaa
       61 tatttaatcg acacacatca ctgtcattaa tgtaaagcaa catagctcac caataataca
      121 atggacaact ctaccggcat tgatcgctgg tcggaggaaa taaggatttt tacatgaact
      181 tgaagaagat ttttttcagt gctgtcactg tttcagttct ctgcgcatta acgggatgtg
      241 actacattga agaaggcaag ccagaaagta gtttactgaa acaacaagaa gaacataata
      301 ataaaattgt gctacttgaa aaacaacaag cacagctaaa gagtcaactc gaaactattc
      361 aaaaacaaca aactggcata ataaacagta caaagacttt aactcacgta attaaatccg
      421 ttaaagacca acaaaatact tttattttta ccgagtttaa cccagcaaaa acgaagtatt
      481 tcatcctgaa caacggttcg gtggctttag cgggtcgagt gttatccatt gacgccacag
      541 agaatggtag tgttattcat atctcactgg tcaacttatt aagtacacct atctcaaaca
      601 ttggttttaa tgcgacatgg ggtggtgaaa aacctgtcga tgccaaagag tttgcccggt
      661 ggcaacaatt acttttcaac acatcaatga aatccacatt gaaattatta ccaggtcaat
      721 ggcaagacat taatttgacc ctgaagggtg tatcgcccaa taacctggga tatctgaagt
      781 tagccatcaa catggaaaat attcagttcg acaatcttcc ctctgctgaa aatcggcaga
      841 aaagaagcaa aaaataaatg cagttagtac gcttataaca aggaatagcg agcatcatta
      901 atgaacaaaa tatttaaagt tatctggaac cctgcgacag ggaattatac tgttaccagc
      961 gaaacggcaa aaagccgtgg caagaaatct gggcgcagta agctgttaat ttctgcgctg
     1021 gttgcgggtg gaatgttgtc gtcgtttggg gcattggcga atgccgggaa tgacaacggt
     1081 cagggtgttg attacggtag tggatcagct ggcgacggct gggttgctat aggcaaaggg
     1141 gcgaaagcaa atacttttat gaacaccagt ggttccagta ctgctgtggg ttatgacgct
     1201 atagctgaag gccaatatag ctctgccatc gggtcaaaaa cccatgcgat tggcggtgca
     1261 tcaatggcct ttggggttag tgcaatatca gaaggcgata gaagtatagc actgggtgcc
     1321 tcttcgtatt cattgggcca atactcaatg gccctcggcc gttattcaaa agcattgggt
     1381 aaattgtcta ttgctatggg ggactcttcc aaagcggaag gagcaaacgc cattgccctg
     1441 ggaaatgcca ctaaagctac tgagattatg agtattgctc ttggcgacac cgccaatgcg
     1501 tcaaaagcgt attcaatggc gctgggagca agtagcgtcg catctgaaga aaacgcaatt
     1561 gccctggggc gtagcagtgt agctagcggt actgacagcc tcgcatttgg cagacaatca
     1621 cttgccagcg cagcgaacgc tattgcgata ggtgctgaga ccgaagccgc tgaaaatgca
     1681 actgctattg gcaataatgc gaaggcaaaa gggactaata gcatggcaat ggggttcgga
     1741 agccttgccg ataaagtcaa tactatcgca ttaggaaatg gcagccaggc tctggcagat
     1801 aatgcaatcg ccataggcca gggcaacaaa gctgatggcg tggatgccat cgctctgggt
     1861 aatggtagcc agtcgagagg cttaaacacc attgccttag gcacagccag taatgcaact
     1921 ggtgataaga gtcttgcgct tggtagtaat agcagtgcca acggtattaa ctctgtcgcg
     1981 ctgggcgcag attccattgc ggatttagac aataccgtct ctgtcggcaa tagttcatta
     2041 aaacgcaaga tcgttaatgt gaaaaatggc gcgatcaagt ctgacagtta cgatgccatt
     2101 aatggttcac agctttatgc cattagcgac tcggtagcaa aaaggcttgg aggaggggct
     2161 gcagtagatg ttgatgacgg tactgttaca gcaccaacct acaatttaaa aaatggtagc
     2221 aaaaataacg taggggctgc gctcgctgta cttgatgaaa acaccctgca atgggaccaa
     2281 accaaaggca aatacagcgc tgctcatggt actagtagcc caactgccag cgtaatcacc
     2341 gatgttgcgg atggcacgat ttcagcctcc agtaaggatg cggttaacgg ttcccaactg
     2401 aaagctacca atgacgatgt cgaagccaac accgccaata tcgctactaa taccagcaac
     2461 attgccacga atacggcaaa tattgccacc aataccacca atatcaccaa cctgacggat
     2521 tccgttggtg accttcaggc tgatgccctg ctctggaacg aaactaaaaa ggcattcagt
     2581 gcagctcacg gccaggatac caccagcaaa atcaccaacg ttaaagatgc cgacctgacg
     2641 gctgacagca ctgatgctgt taacggctct cagctgaaaa ccaccaacga tgctgtggcg
     2701 acgaatacca ccaatatcgc caataacact tccaatattg ccactaacac caccaacatc
     2761 tctaacctga ctgagacggt gactaatctt ggtgaggatg cgctgaaatg ggataaggac
     2821 aatggtgtat tcacggcagc tcatggcacc gagaccacca gcaaaatcac caacgttaaa
     2881 gatggcgacc tgacgactgg cagcaccgat gccgttaacg gctctcagct gaaaaccacc
     2941 aacgatgccg tggcgacgaa taccaccaat atcgccacta acaccaccaa catctctaat
     3001 ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt
     3061 gtcttcactg cagctcatgg caacaatacc gccagcaaaa tcaccaatat cctggacggc
     3121 acagtcactg caaccagttc cgatgccatt aacggtagcc agctttatga cttaagcagc
     3181 aatatcgcca cctacttcgg cggcaatgct tctgtgaata ctgacggtgt gtttaccggt
     3241 ccaacctaca aaatcggtga aacaaattat tataacgtcg gcgatgcact ggctgcgatt
     3301 aactcctcat ttagcacgtc tctcggcgat gctctgcttt gggatgccac cgcaggtaaa
     3361 ttcagtgcca aacacggtac taatggtgac gcaagcgtga tcactgatgt cgcagatggt
     3421 gaaatttcag actccagttc tgacgcagta aacggctcac aactccacgg cgtgagcagt
     3481 tatgttgttg atgcgctggg gggtggtgcc gaagtcaatg cagacggcac catcactgcg
     3541 ccgacgtaca ccattgctaa tgctgattac gataatgtcg gtgatgccct gaatgctatc
     3601 gataccactc ttgacgacgc tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt
     3661 agcgccgctc acggaaaaga taaaactgcc agtgtaatca ctaacgtcgc taacggtgca
     3721 atctctgctg ccagcagcga cgcgattaac ggctcacaac tctataccac caataagtac
     3781 atcgctgatg cgctgggtgg tgacgcagaa gtcaacgctg acggcaccat caccgcaccg
     3841 acttacacca ttgcgaacgc cgagtacaac aacgtcggtg acgccctgga tgcgcttgat
     3901 gataacgccc tgctgtggga tgagactgcc aatggcggtg ctggagccta caatgccagc
     3961 catgacggta aagccagcat catcactaat gtcgctaatg gcagtattag tgaggacagt
     4021 accgatgcag tgaacggttc tcagttgaat gcgacgaata tgatgattga gcagaacacc
     4081 caaattatca atcagctcgc tggtaacacc gacgcaacct atatccaaga aaacggtgcg
     4141 ggtattaact atgtgcgtac taacgacgac ggcttagcgt tcaacgacgc cagcgcacag
     4201 ggtgttggcg ctacagctat aggttataac tctgtcgcca aaggcgatag cagcgtagct
     4261 attggtcagg gcagctacag cgacgttgat acgggtatcg ccctgggtag cagctctgtt
     4321 tccagccgag tgattgccaa aggctcccgt gacaccagca taacggaaaa tggcgttgtt
     4381 attggttacg acaccacgga tggcgaactg ctcggtgcat tgtctatcgg tgatgacggt
     4441 aaatatcgtc aaatcatcaa cgtagccgat ggttccgaag cccatgacgc cgttacggtt
     4501 cgtcaattgc agaatgcgat tggtgcggtc gcaaccacgc cgactaaata cttccacgct
     4561 aattcaacgg aagaagattc actggcagtg ggaactgact cgctggcaat gggtgcgaaa
     4621 accatcgtga atggcgataa aggtattggt atcggttatg gtgcctacgt ggacgcgaat
     4681 gcacttaacg gcattgccat tggtagcaat gcgcaagtca ttcatgtcaa cagtattgcg
     4741 ataggtaatg gttctacgac cactcgtggc gctcaaacca attataccgc ctacaacatg
     4801 gacgcaccgc agaactctgt cggtgaattc tcagtcggta gtgcggatgg tcaacgtcag
     4861 atcactaacg tcgcagcagg ttcggctgat accgatgcgg tcaacgtggg tcagttgaaa
     4921 gtaacggatg cgcaggtttc ccagaatacc cagagcatta ctaacctgga taatcgggta
     4981 acgaatcttg attcacgcgt caccaatatc gaaaacggta ttggcgatat cgtcaccacc
     5041 ggtagcacca agtacttcaa gaccaatacc gatggtgtag atgccagcgc gcagggtaaa
     5101 gatagcgtcg cgattggttc tggctccatt gctgccgcgg ataacagcgt cgcactgggt
     5161 acagggtctg tggcaaccga agaaaatacg atctctgtag gctcatctac taatcaacgt
     5221 cgtatcacca acgtagccgc aggtaaaaac gacaccgatg ctgttaacgt ggcacagttg
     5281 aagtcttccg aagctggcgg tgtgcgttac gacaccaaag ctgatggttc tatcgactat
     5341 agcaatatca ccctcggtgg cggcaacggc ggtacgactc gtatcagcaa cgtctccgct
     5401 ggcgtcaaca acaacgacgc ggtgaattac gcgcagttga agcaaagcgt gcaggaaacg
     5461 aagcaataca ccgatcagcg aatggttgag atggataaca aactgtctaa aactgaaagc
     5521 aagttgagcg gtggtatcgc ttctgcaatg gcaatgaccg gtctgccgca ggcttataca
     5581 ccgggtgcca gcatggcttc tattggtggc ggtacttaca acggtgaatc ggcagttgct
     5641 ttaggtgtat cgatggtgag cgccaatggt cgttgggtct acaaattaca aggtagtacc
     5701 aatagccagg gtgaatactc cgccgcactc ggtgccggta ttcagtggta atgatccatt
     5761 aagttagtgt gactaagggc aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag
     5821 ataaggcgct ccgggtcaac aatctaaggg caattctctg atgaggattg cccttttctt
     5881 taccagacat ctccccccac aagaattggc cctaccaatc cttcgcttat ctgacctctg
     5941 gttcacaatt ccccaattaa aactcacatc aatattgcca atacataaca tttagttaac
     6001 cattcattgt cattatccct acacaacaca attggcagtg ccacttttac acaacgtgtg
     6061 acaaggagat gagcaacaga ctcattacac gatgtgcgtg gactccagga gacctgcaat
     6121 gaatctctgg caacaaaact acgatcccgc cgggaatatc tggctttcca gtctgatagc
     6181 atcgcttccc atcctgtttt tcttctttgc gctgattaag ctcaaactga aaggatacgt
     6241 cgccgcctca tggacggtag caatcgccct tgccgtggct ttgctgttct ataaaatgcc
     6301 ggtcgctaac gcgctggcct cggtggttta tggcttcttc tatggtttgt ggcccatcgc
     6361 gtggatcatt attgcagcag tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga
     6421 catcattcgc tcgtctattc tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt
     6481 cggtttctgt ttcggcgctt tccttgaagg agccgcaggc tttggcgcac cggtagcaat
     6541 taccgccgca ttgctggtcg gcctgggttt taaaccgctg tacgccgccg gactgtgcct
     6601 gattgttaac accgcgccag tggcatttgg cgcgatgggc attccaatcc tggttgccgg
     6661 acaagtaaca ggcatcgaca gctttgagat tggccagatg gtggggcggc agctaccgtt
     6721 tatgaccatt atcgtgctgt tctggatcat ggcgattatg gacggctggc gcggtatcaa
     6781 ggagacgtgg cctgcggtcg tggttgcggg cggctcgttt gccatcgctc agtaccttag
     6841 ctctaacttc attgggccgg agctgccgga cattatctct tcgctggtat cactgctctg
     6901 cctgacgctg ttcctcaaac gctggcagcc agtgcgcgta ttccgttttg gtgatttggg
     6961 ggcgtcacag gttgatatga cgctggccca caccggttac actgcgggtc aggtgttacg
     7021 tgcctggaca ccgttcctgt tcctgacagc taccgtaaca ctgtggagta tcccgccgtt
     7081 taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc
     7141 gtacctcgat aaactggttg cccgtatgcc accagtggtc agcgaggcta cagcctatgc
     7201 cgccgtgttt aagtttgact ggttctctgc caccggcacc gccattctgt ttgctgcact
     7261 gctctcgatt gtctggctga agatgaaacc gtctgacgct atcagcacct tcggcagcac
     7321 gctgaaagaa ctggctctgc ccatatactc catcggtatg gtgctggcat tcgcctttat
     7381 ttcgaactat tccggactgt catcaacact ggcgctggca ctggcgcaca ccggtcatgc
     7441 attcaccttc ttctcgccgt tcctcggctg gctgggggta ttcctgaccg ggtcggatac
     7501 ctcatctaac gccctgttcg ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc
     7561 tgatctgttg ctggttgccg ccaataccac cggtggcgtc accggtaaga tgatctcccc
     7621 gcaatctatc gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt
     7681 ccgctttact gtcaaacaca gcctgatctt cacctgtata gtgggcgtga tcaccacgct
     7741 tcaggcttat gtcttaacgt ggatgattcc ttaatgattg ttttacccag acgcctgtca
     7801 gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga agcgggcatg
     7861 aagttgcccg ctgagcgcca actggcgatg caactcggcg tatcacgtaa ttcactgcgc
     7921 gaggcgctgg caaaactggt gagtgaaggc gtgctgctca gtcgacgcgg cggcgggacg
     7981 tttattcgct ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc gctaaaaaca
     8041 ctgatggccg atgatccgga ttacagtttc gatattctgg aagcccgcta cgccattgaa
     8101 gccagcaccg catggcatgc ggcaatgcgc gccacacctg gcgacaaaga aaagattcag
     8161 ctttgctttg aagcaacgct aagtgaagac ccggatatcg cctcacaagc ggacgttcgt
     8221 tttcatctgg cgattgccga agcctcacat aacatcgtgc tgctgcaaac catgcgcggt
     8281 ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc agcggatgta tctggtgcca
     8341 ccggtttttt cacaactgac cgaacaacat caggctgtca ttgacgccat ttttgccggt
     8401 gatgctgacg gggcgcgtaa agcaatgatg gcgcacctta gttttgttca caccaccatg
     8461 aaacgattcg atgaagatca ggctcgccac gcacggatta cccgcctgcc cggtgagcat
     8521 aatgagcatt cgagggagaa aaacgcatga ttatttccgc agccagcgat tatcgcgccg
     8581 cagcgcaacg cattctgccg ccgttcctgt tccactatat ggatggtggt gcatattctg
     8641 aatacacgct gcgccgcaac gtggaagatt tgtcagaagt ggcgctgcgc cagcgtattc
     8701 tgaaaaacat gtccgactta agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc
     8761 cggtggcgct aggtccggta ggtttgtgtg gcatgtatgc gcgacgcggc gaagttcagg
     8821 cagccaaagc ggcggatgcg catggcattc cgtttactct ctcgacggtt tccgtttgcc
     8881 cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg gttccagctt tatgtactgc
     8941 gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc
     9001 tggttttcac cgtggatatg ccgacaccgg gagcgcgtta tcgtgatgcg cattctggaa
     9061 tgagcggccc gaacgcggca atgcgccgct atttgcaggc ggtgacgcat ccgcaatggg
     9121 cgtgggatgt gggcctgaac ggtcgtccgc atgatttagg taatatctca gcttatctcg
     9181 gcaaaccgac cggactggaa gattacatcg gctggctggg aaataacttc gatccgtcca
     9241 tctcatggaa agaccttgag tggatccgcg atttctggga tggcccgatg gtgatcaaag
     9301 ggatcctcga tccggaagat gcgcgcgatg cagtacgttt tggtgctgat gggattgtgg
     9361 tttctaacca cggtggccgc cagctggacg gtgtactctc ttccgctcgt gcactgcctg
     9421 ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga attcgtaacg
     9481 ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt
     9541 tcttgtatgc gctggcaaca gcgggccagg cgggtgtagc taacctgcta aatctgatcg
     9601 aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa atcgatcaat gaaattacgc
     9661 aggattcgct ggtgcagggg ctgggtaaag agttgcctac ggcactggct ccgatggcga
     9721 aagggaatgc ggcatagtcg tttgagcccc ctcaccctaa ccctctccct cagggagtgg
     9781 ggaccgttcg gcgctgtatg tactccctca ctctgaaacg acaccgcact ctttttttct
     9841 ccctcgcccc tccggggtga ggggaaaagg ccgcactgct caccaatttt ctgctatcct
     9901 gcccccgcac taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc
     9961 aaatactggc aatatcatcc gtctttgcgc taataccggc tttcgtctgc atatcatcga
    10021 accgatggga tttgcctggg acgataagcg cctgcgccgc gcggggctgg actatcacga
    10081 gtttaccgcc gttacgcgtc atcatgactt tcgcgcgttc ctcgaagcag aaaatcccca
    10141 gcgcctgttt gccctcacca cgaaaggtac gcctgctcac agcgccgtaa actatcagga
    10201 tggcgattat ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc
    10261 cctgcccgct gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaacct
    10321 gtccaatgcg gtgtcagttg tggtgtatga agcctggagg cagctgggat atccgggagc
    10381 gttgttgaga gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc
    10441 acaggacatc agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg
    10501 tccatatcca ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca
    10561 gcggcggttg tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg
    10621 ccgcgaccaa cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca
    10681 cgaattttcg ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc
    10741 agaatcgata cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg
    10801 tcgagcataa tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca
    10861 gaaacctggt tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga
    10921 ccgatgcgat aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat
    10981 ttatcgactg ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc
    11041 atttccgggt cagcggcgta ggcttcttcc accacttcac ggatagcaat agcaggcata
    11101 attggcgatg acagcttgtt cgccagcatg tagctcagtg cactaccaag gttttcgtgc
    11161 ttgagtagcg tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg
    11221 gcttcggctt taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc
    11281 acacgatgag ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt
    11341 tccctggggt tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata acgtcaatgc
    11401 tgcctcgcgc gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat
    11461 ttcaacgccg aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac
    11521 cacctgacca atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc
    11581 aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc accagatcgc caagccccgc
    11641 catgcccata aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc
    11701 cagcccacgg gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcggacat
    11761 ccccgcgcca atggcaatga cgtttttcac cgcaccgcca agctgcacac cgatgaaatc
    11821 cgggttgctg taaacgcgga aacttttgcc acagtgcagc aattgttgga gatcatcggc
    11881 aaaggtctgg tcggtagagg ccaacgaaat agccgtcggt aaacctgccg ccagttcttt
    11941 cgcaaacgtt gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc
    12001 cacgtcctgt aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg
    12061 cgcatcagga cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg
    12121 tacgacgacg agaatattac ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg
    12181 gagcgtatcg ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag
    12241 cgttgcgata tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag
    12301 ggtaatggca agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg
    12361 ttggttcatc aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag
    12421 ttcatgaaca gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg
    12481 gataccatgc tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg
    12541 cccaggcaat gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc
    12601 tgaacttcac acaggaacgc ggtttcttcg cccagggagg ccgttacggt aacacgcagt
    12661 accacttcgt atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact
    12721 tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc
    12781 ttggtataaa tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt
    12841 agaaaaccct taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt
    12901 atttcagcag gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc
    12961 caatgtgctg tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca
    13021 tctcttcacg cttggcggca ttgccatcga tcggcagctc ctggaaactc acgcccttgc
    13081 tgctcagcag tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa
    13141 cattggccat aaattagctc ctgttttttt accctgaaat ttcatgttgc agggcggcag
    13201 caaaatggag tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta
    13261 acggcctgca tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt
    13321 cgccagccca gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg
    13381 tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccggtt
    13441 tgtctttgtg cttctcaagc tcaccaacat tgttggcttt gatttcactc ggcaacagat
    13501 tgatagaacc tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca
    13561 cagcgtcttc tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg
    13621 aggtcaggct cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac
    13681 tcagtatggg atgacggcca acaaattgca taatttcttg catggggggt aacaactccc
    13741 gacgtagtga ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc
    13801 agcgcgttaa ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca
    13861 tcggcttgcc gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct
    13921 gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta
    13981 aaaaacctat ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata
    14041 acgccatttt tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata
    14101 ccctgatcga cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg
    14161 aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg
    14221 acgttgaaat caaagatcgc gctttctttg ctaatccagt gctgactggc gcggtagata
    14281 aagcgaaaaa cgcaggtaaa gcagtacaca ttatgggtct gctctccgta ggcggcgtac
    14341 acagccacga agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa
    14401 aaatctacct gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct
    14461 cgctgaaaaa atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca
    14521 ttggtcgtta ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg
    14581 acctgctgac tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg
    14641 cagcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag
    14701 gtcagccaga tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg
    14761 accgcgcgcg tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta
    14821 agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg
    14881 cggttgctta cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg
    14941 acaaaactca gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca
    15001 acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag
    15061 tggctaccta cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg
    15121 cggccatcaa gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg
    15181 taggtcacac cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg
    15241 tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc
    15301 acggtaacgc tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca
    15361 acctgccagt tccgctgatt tatgttggtg ataagaacgt gaaagcggtt gaaggcggca
    15421 aactttctga catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga
    15481 tgactggtaa gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta
    15541 ataccatgac acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca
    15601 gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc
    15661 aactcaaatc tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac
    15721 aacaacgcgc aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca
    15781 cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga
    15841 tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg
    15901 cgcaactgga tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg
    15961 gtgaagaaag ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc
    16021 gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg
    16081 aactggaaga gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg
    16141 cgaaactgac tcaggcgctg aacgagcgta aaaagacgct ggcagggctg gagtcttcca
    16201 tccagcaagg tcagcaacag ttgagcgagc tgcgcgccaa cgaatcccgt ctgcgtaaca
    16261 gcattgcccg tgcggaagcc gcggcgaaag cgcgtgcaga acgagaagca cgtgaggccc
    16321 aggcggttcg cgaccgccag aaagaagcga cgcgtaaagg caccacctac aaaccgactg
    16381 aaagcgaaaa atcgctgatg tcccgtaccg gtggtctggg cgcgccgcgt ggtcaggcat
    16441 tctggccggt tcgcgggccg acgctgcatc gctatggcga acagctacag ggtgaattac
    16501 gctggaaagg gatggtgatt ggtgcctctg aaggtactga agttaaagcg attgccgacg
    16561 gtcgggtgat tctggctgac tggctgcaag gctacggtct ggtggtggtg gttgagcatg
    16621 gtaaaggcga catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc
    16681 aggttcgcgc gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt
    16741 cactctattt cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa
    16801 gataagtttt gtttccattt cgtcgtaacg ttcttgcatt tgccactctg ttggcgctct
    16861 cctcccccgt acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc
    16921 acaacgaaaa ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt
    16981 caccgcacgc cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc
    17041 atctgccaat ggcaccgttg agtaaacagc cgctggagaa aaatacgctg cgcccggaga
    17101 tgagcagcga cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg
    17161 tggggatcaa caaccacatg ggtagcaaga tgacctctaa cctgtttggt atgcagaaag
    17221 tgatgcaggc gctggagcgt tacaatcttt acttcctcga cagtgtaacc attggtaata
    17281 cccaggcgat gcgcgccgcg caaggcactg gcgtgaaggt tattaaacgg aaggtgttcc
    17341 tcgacgattc acagaacgaa gcggacatcc gtgtgcagtt caatcgcgca attgatctgg
    17401 cgcgtcgcaa cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc
    17461 tacaacagat ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc
    17521 tgaatgaacc gcaggttgat acgtctacac cgccgaaaaa cgctgtgcct gatgcaccgc
    17581 gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg
    17641 ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact
    17701 tccagcatca gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg
    17761 caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca
    17821 taagcggaaa gaccacagta acgcctggta acccaccttc acgctgcgca cattggtaat
    17881 caggcgctta tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat
    17941 atccggctct ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg
    18001 aaattccgga taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg
    18061 ggtaatctta atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg
    18121 actcactgac gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg
    18181 cagcgcatta aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat
    18241 aaatttaatg ttatttttaa caataacatc gcggcgataa acccccatcc agacaacgtg
    18301 agtccaacgg cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacaccggt
    18361 tgagcgaagg cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaacacca
    18421 gtcagcgttg cactgcgcca cgtcgaggtc gtcctctaac gccatggtca tcagcgtttc
    18481 gtacatggtg ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc
    18541 cacctcgatc ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg
    18601 aacgtgcgga tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc
    18661 atcgttaata ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca
    18721 agtgcggaaa tcatcgcccg cattatataa cggaataata acactaagtt tactggtgct
    18781 gttcatcata aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt
    18841 aagaaactat taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag
    18901 agagataatg agaacagcta tataagagat cagcgcaatt acgaactctt tattacactg
    18961 acaggatttt acatatttaa cggattatcc tcaggcaaaa gggctggaat accagccctt
    19021 gttcgtgtta atcccagctc agaattactt tcccggactg gcccgaacgc atagcgtcaa
    19081 agcccttctg gaaatcatca atagagaaac gatgggtaat gatcggcgag agatcgaggc
    19141 cagactgaat cagcgccgcc atcttgtacc aggtttcaaa catctcacga ccgtaaatac
    19201 ctttaatgaa caagccttta aagatcactt tggtccagtc gatagacata tcagacggcg
    19261 gaatacccag catcgcaata cggccgccgt ggttcatggt gtcaagcatg gtacgaaacg
    19321 ctggcggcgc accggacatt tccagaccga catcaaagcc ttcggtcatg cccagttctg
    19381 ccatcacatc attaaggttt tctttcgcga cgttaaccgc acgggtgata cccattttgc
    19441 gcgccagctc aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt
    19501 gtttcgccac cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt
    19561 cgcccaccag atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg
    19621 aagccaggtc gtcggagata ttgtctggga ttttgaaggc gttgaacgcc gggatcacca
    19681 gatattcggc gaagcagccc ggacggttaa cgccgacgcc gatcgtgttg cggcacagat
    19741 gggtacgacc gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa
    19801 cgcgatcgcc gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat
    19861 attcatggcc tacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat
    19921 agatgtgaac gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat
    19981 gcccgagttc cggtacagga acgtcggtca tccagatgcc ctcttccgct ttcagtttgg
    20041 ataacgcttt catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga
    20101 atgcttctac tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa
    20161 tacgcgcctg acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt
    20221 tttgcagctc acgggcaaat ttctgcgcca ctactgcatc gccaagcatg accggaataa
    20281 tggcatgatc ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactggc
    20341 gcgcgttcgc ccacagacgg tcacgcagtt cgctgcccgc ttcgaccatc tccagtactt
    20401 tgatggacgc ggcaacaatg gccggtgcca gcgagttgga gaacaggtac ggacgagaac
    20461 gctggcgcag ccactcaacc acctctttgc gcgccgcggt ataaccacca gaagccccgc
    20521 ccagcgcttt accaagcgta ccggtgataa tatcgacccg gcccatcaca tcgcagtatt
    20581 catgggaacc acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca
    20641 ccagggcatc gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt
    20701 ccatcgagaa tacaccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt
    20761 ctttcagacg agcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt
    20821 tacacagacg cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt
    20881 cttccgcgcc cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga
    20941 gaatcgcatc ttccatcccc aggaaggccg ccagtttttg ttcaagctct ttatggctgt
    21001 cctgagtgcc gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg
    21061 cctttgccgc cgcaatcaga tcaggatgat tcgccagccc gagatagttg ttggcacaaa
    21121 agttaatgac gtggcttcca tcagccacag tgatatctgc ttgctgcgca gacgtaataa
    21181 tgcgctcttc tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct
    21241 gataaaattc tccacgcatt gcgattctcc agacagggca aattccagca catattaccc
    21301 aaacttatag gtcgcgacga gatgacgcgt taacacttct gcaaaattca ggataaataa
    21361 cgtgtaactt gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg
    21421 ggcataatga tgattgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat
    21481 catttttcct atctcagttg gtatcaaaac gctacccact aatggaggga aacctatttt
    21541 cttgaaagga aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag
    21601 cgaaacaaat cgaaccattt tcctctcccc agatagttta ggttgaagag atcggggcgt
    21661 ttgtaatagc acatgacaaa aatgccctgg tcatcatcca caatgttatt atttagtgtt
    21721 attttttgac tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca
    21781 attgcgcccc caataatata tacatgattg ccaatcatga aatcaaaaac ctgttgttga
    21841 gatgtgacag taagcccttt ttttatagtg aaaagatgca tcttactttc atcaaatggg
    21901 aaatcccaaa ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc
    21961 cacgcaacaa gaggcgtttt cactaagccc atgttgatag ctttatttac aaaataggcc
    22021 ttaaggttac ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga
    22081 ggctcaagtc tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata
    22141 tatctaaatt tcttttttat atcgataacg ataaccgttg ttggttttcc gttgcgaatc
    22201 gcttcaaccc ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg
    22261 gccagacgct caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac
    22321 cctttattag cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg
    22381 gatgatttca ttactaataa gaccattaat ataaaaaaag aattatatca taaatattaa
    22441 cagaatgtat actgtatatt atccattcaa atgcttccgt tatttatcat acatcggaat
    22501 attgatacta aagcactatt cacatgcaaa accaacatcc gccatgaagg actagctaaa
    22561 acccaaacta gtttgttgca attagcatcc ttgcacctct atgtaaaggg ctgaagggat
    22621 tcggatgtga tggtatgatt acagacattc gtgtctgaga ttgtctctga ctccataatt
    22681 cgaaggttac agttatgatc atcgttaccg gcggcgcggg ctttatcggc agcaacatcg
    22741 ttaaagccct gaatgataaa ggcatcaccg atattctggt ggtggacaac ctgaaagacg
    22801 gcaccaagtt tgtgaacctg gtggatctga atatcgcaga ctatatggat aaggaagact
    22861 tcctgatcca gattatggct ggcgaagagt tcggcgatgt cgaagcgatt ttccacgaag
    22921 gcgcgtgctc ttccaccacc gagtgggacg gcaagtatat gatggataac aactatcaat
    22981 actccaagga gctgctgcac tactgcctgg agcgtgaaat cccgttcctg tacgcttctt
    23041 ccgcagccac ctacggcgga cgcacctccg actttattga atcccgcgag tacgaaaaac
    23101 cgttgaacgt ctacggttac tcaaaattcc tgtttgatga atatgttcgc caaatcctgc
    23161 cggaagcgaa ctcgcagatt gttggcttcc gctatttcaa cgtttatgga ccgcgtgaag
    23221 gccataaagg cagcatggcg agcgtcgctt tccatctcaa cacccagctt aataacggtg
    23281 aatcaccgaa gctgttcgaa ggtagcgaga actttaaacg cgacttcgtc tatgttggcg
    23341 atgtggcaga tgtaaacctg tggttcctgg aaaatggcgt ttccggcatc ttcaatctcg
    23401 gcaccggtcg tgcggaatcc ttccaggctg tagctgatgc tacgctggct tatcacaaga
    23461 aaggccagat cgaatacatt ccgttcccgg ataagctgaa aggccgctac caggcgttca
    23521 ctcaggcaga tctgacaaat ctgcgcgcgg cgggttacga caaaccgttc aaaaccgttg
    23581 ctgaaggtgt aacggaatac atggcctggc tgaatcgcga cgcataagag ctctgcatga
    23641 aaatactggt gatcggcccg tcttgggttg gcgacatgat gatgtcgcaa agtctctatc
    23701 gcacgctcca ggcgcgctat ccccaggcga taatcgatgt gatggcaccg gcatggtgcc
    23761 gtccattatt atcgcggatg ccggaagtta acgaagctat ccctatgcct ctcggtcacg
    23821 gagcgctgga aatcggcgaa cgccgcaaac tgggtcatag cctgcgtgaa aagcgctacg
    23881 accgcgccta cgtcttacct aactccttca aatccgcatt agtgcctttc ttcgcgggta
    23941 ttcctcatcg caccggctgg cgcggcgaga tgcgctacgg tttactcaac gatgtacgcg
    24001 tgctcgataa agaagcctgg ccgctaatgg tggaacgcta tatagcgctg gcctatgaca
    24061 aaggcattat gcgcacagca caagatctgc cgcagccatt gttatggccg cagttgcagg
    24121 tgagcgaagg tgaaaaatca tatacctgta atcaattttc gctttcatca gaacgtccga
    24181 tgattggttt ttgcccgggt gcggagtttg gtccggcaaa acgctggcca cactaccact
    24241 atgcggagct ggcaaagcag ctgattgatg aaggttatca ggtggttctg tttggctcgg
    24301 cgaaagatca tgaagcaggc aatgagattc ttgccgcttt aaataccgag cagcaggcat
    24361 ggtgtcggaa cctggcgggg gaaacacagc ttgatcaagc ggttatcctg attgcagcct
    24421 gtaaagccat tgtcactaac gattctggcc tgatgcacgt tgcggcggcg ctcaatcgtc
    24481 cgctggttgc cctgtatggt ccaagtagcc cggacttcac accgccgcta tcccataaag
    24541 cgcgcgtgat ccgtctgatt accggctatc acaaagtgcg taaaggtgac gctgcggagg
    24601 gttatcacca gagcttgatc gacattactc cccagcgcgt actggaagaa ctcaacgcgc
    24661 tattgttaca agaggaagcc tgacggatgc gggttttgat cgttaaaaca tcgtcgatgg
    24721 gcgatgttct ccatacgttg cccgcactca ctgatgccca gcaggcaatc ccagggatta
    24781 agtttgactg ggtggtggaa gaagggttcg cacagattcc ttcctggcac gctgccgttg
    24841 agcgagttat tcctgtggca atacgtcgct ggcgtaaagc ctggttctcg gcccccataa
    24901 aagctgaacg caaagcgttt cgtgaagcgc tacaagcaga gaactatgac gcagttatcg
    24961 acgctcaggg gctggtaaaa agcgcggcac tggtgacacg tctggcgcat ggcgtaaagc
    25021 atggattgga ctggcaaacc gctcgcgaac ctttagccag cctgttttac aattgtaagc
    25081 atcatattgc aaaacagcag cacgccgtag aacgcacccg cgaactgttt gccaaaagtt
    25141 tgggctatag caaaccgcaa acccagggcg attatgctat cgcacagcat tttctgacga
    25201 acctgcctac agatgctggc gaatatgccg tatttcttca tgcgacgacc cgtgatgata
    25261 aacactggcc ggaagaacac tggcgagaat tgattggttt actggctgat tcaggaatac
    25321 ggattaaact tccgtggggc gcgccgcatg aggaagaacg ggcgaaacga ctggcggaag
    25381 gatttgctta tgttgaagta ttgccgaaga tgagtctgga aggcgttgcc cgcgtgctgg
    25441 ccggggctaa atttgtagtg tcggtggata cggggttaag ccatttaacg gcggcactgg
    25501 atagacccaa tatcacggtt tatggaccaa ccgatccggg attaattggt gggtatggga
    25561 agaatcaagt agagtgtcgt tctacaagta tgtctcttgc agatttgcca gctcaaaccg
    25621 tttttcaaaa tttaaatctt gaaataataa ccaataagtt gacatcggag ataagatgac
    25681 ctcaacatta tttttctctc tcgagaaaaa aaactggata gcgtactgga acagagctct
    25741 cgtattctta ttcattacca cctatttttt gggtgggatt acaaggtata aacatcttat
    25801 tgttattctt atgacaataa cgacaatcgt ctatctctgc aaacggccaa aacactatct
    25861 ctcactattt aaaacatttc tttttggtag tgttgccata ttaactattg ctgcattgct
    25921 gtcacttctt caatcccctg atgcaggtgc tagcatgaag gaaattttca aagctattat
    25981 tgagaatact ttactatgca caatagcaat accgatcata ttgagagacg agaaaagaga
    26041 agatgtcgaa aaaatcgttt tcttctcatt tattagtgcg ttgggcttac gctgtttttc
    26101 tgaattgatt acctattata aggactatca acaagggata atgccattcg cagattatag
    26161 acaccgtagc atttctgact cgatggtctt tttattccct gcattgttaa atctctggct
    26221 tatcaaatca gcaaaatacc gcatttcttt tgtggttcta agcgttattt ttatttttct
    26281 gatattagga actttatcca gaggggcctg gctttccgtg ttagtcattg gattaatatg
    26341 gattctgatg tttaaacaat ggaagttact attagtagga gtaatggttg ccatcattgc
    26401 attgtcggtt attttcacac ataaggagat gaccgcaaag ctaacgtata aacttcaaca
    26461 aactaatagt tcttatcgct atgcaaatgg tactcaaggc agcgcactcg atctaatatt
    26521 agaaaatcct gttattggtt atggttacgg taacgttgca tataaagatg tctataataa
    26581 acgtgtcatt gattatccag aatggacctt tagacaatca atagggccac ataattttgc
    26641 gctattcatc tggtttggca ctggtttatt agggctggta agtcttatga tgctatactg
    26701 tgcaatattg aaagagtgta taaaaaatgg cgtcaagaat aaatatcgct caccatataa
    26761 tgcatattat ataatcttac tatcttttat aggttatttt gttatccgtg gaaacgtaga
    26821 acaaattgaa ccaaatttat taggcgttta cgccggctta ttattagcga tgaaaaacaa
    26881 gtaaaaaata aaaaaggctg cataatgcgg cctttttatt tattcaaacc aattatgaat
    26941 aacctcttcg aatctttgag ttacgccatc ccaactgtag tgttcaaaca caaagtcctg
    27001 accttgcttt gcaactgctg ttaattcagg attcgctaac gtttttaaaa tatcgctgct
    27061 tatggagtct gcagtcatag gttcttttaa atggaatccc gtagtatttt ctttgacaaa
    27121 ttccgtcatc cccccacgcg tactcactaa aacaggctta ccagcaccca tagcttcaat
    27181 tgccaccatg caaaatggtt cctggaactg ggaaggaatg acgactaaat ctgccagtgg
    27241 gtaataacaa tgcatctttt caggcggtat gcttcctaac ataatgcaac gatccttcaa
    27301 acgttttgca atttccctga cattgcgttg atatgcacct ttgtcacttt tactcatttc
    27361 tgtataatca ccaatcacaa ctaattttaa atttttgtga gcagcggcca atttttcgaa
    27421 tgattgcatc aacagcaata tgcctttgtc agggacgata cgcccagcat aaaaaataat
    27481 tttctcttca ggggagatat taagttcgct tctagtgatc ggctgaaaat tagattgata
    27541 ggtttctaaa tcaattccat ttggtacaat ctcaatatct gcatttgcta aataagattg
    27601 gtaatacttt tccaagtata aactaggcac tatcatttta acattttgct caagcaattt
    27661 tggttcaaat gcattatgca tatgtataac cactcttgcc tgaggagctc gcttacgaat
    27721 ttgagtgtat agcttcatgc tattatgaac tacaataaca ctttctttcg ttataggaaa
    27781 atcatgtgca atattcaata ttctctgtga atatggaaga ggatcaagtc ttgtccattt
    27841 ttgaaataac cgcttatata tgcgactgaa tccaacccta tgaatactac aattatcatt
    27901 aacaaaggtg taatttgagt atcctggatt tttgatacaa acaatacgat ttggaaaatt
    27961 ggtacgctga gcaacctgat acatccacgt ttcaacagca gccgctccgc gaggaggaat
    28021 tgaaaaaata ggagtaaccg taaatattat tttatcaacc atctcgtata ttaacctttc
    28081 ataacattat atttaattgc tgtaaaaaca ccatcgagaa attttttctg gtaaagcaag
    28141 tgtttatatt tttctttgta ctcatgtttt ttaacagctt ttttataagg tatatttctc
    28201 catggtgata tattataaat attacgaaaa taatctgcag aggcgtagtt cgcccaatca
    28261 tgccatggct tagttatacc ag
//
LOCUS       DAZFYC010000060        28253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFYC010000060 DAZFYC010000000
VERSION     DAZFYC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.060"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..934
                     /gene="ygcG"
                     /locus_tag="ACHKW5_002813"
                     /pseudo
     CDS             62..934
                     /gene="ygcG"
                     /locus_tag="ACHKW5_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(994..2292)
                     /gene="eno"
                     /locus_tag="ACHKW5_002814"
     CDS             complement(994..2292)
                     /gene="eno"
                     /locus_tag="ACHKW5_002814"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO7512173.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2380..4017)
                     /gene="pyrG"
                     /locus_tag="ACHKW5_002815"
     CDS             complement(2380..4017)
                     /gene="pyrG"
                     /locus_tag="ACHKW5_002815"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO7512174.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPECKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4245..5036)
                     /gene="mazG"
                     /locus_tag="ACHKW5_002816"
     CDS             complement(4245..5036)
                     /gene="mazG"
                     /locus_tag="ACHKW5_002816"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO7512175.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5107..5442)
                     /gene="mazF"
                     /locus_tag="ACHKW5_002817"
     CDS             complement(5107..5442)
                     /gene="mazF"
                     /locus_tag="ACHKW5_002817"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO7512176.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5442..5690)
                     /gene="mazE"
                     /locus_tag="ACHKW5_002818"
     CDS             complement(5442..5690)
                     /gene="mazE"
                     /locus_tag="ACHKW5_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO7512177.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5768..8002)
                     /gene="relA"
                     /locus_tag="ACHKW5_002819"
     CDS             complement(5768..8002)
                     /gene="relA"
                     /locus_tag="ACHKW5_002819"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO7512178.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8050..9351)
                     /gene="rlmD"
                     /locus_tag="ACHKW5_002820"
     CDS             complement(8050..9351)
                     /gene="rlmD"
                     /locus_tag="ACHKW5_002820"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO7512179.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9408..12164
                     /gene="barA"
                     /locus_tag="ACHKW5_002821"
     CDS             9408..12164
                     /gene="barA"
                     /locus_tag="ACHKW5_002821"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO7512180.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12396..13736)
                     /gene="gudD"
                     /locus_tag="ACHKW5_002822"
     CDS             complement(12396..13736)
                     /gene="gudD"
                     /locus_tag="ACHKW5_002822"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO7512181.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13757..15097)
                     /gene="gudX"
                     /locus_tag="ACHKW5_002823"
     CDS             complement(13757..15097)
                     /gene="gudX"
                     /locus_tag="ACHKW5_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO7512182.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15099..16451)
                     /gene="gudP"
                     /locus_tag="ACHKW5_002824"
     CDS             complement(15099..16451)
                     /gene="gudP"
                     /locus_tag="ACHKW5_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO7512183.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16886..17335)
                     /gene="yqcA"
                     /locus_tag="ACHKW5_002825"
     CDS             complement(16886..17335)
                     /gene="yqcA"
                     /locus_tag="ACHKW5_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO7512184.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17353..18135)
                     /gene="truC"
                     /locus_tag="ACHKW5_002826"
     CDS             complement(17353..18135)
                     /gene="truC"
                     /locus_tag="ACHKW5_002826"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO7512185.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18135..18464)
                     /gene="yqcC"
                     /locus_tag="ACHKW5_002827"
     CDS             complement(18135..18464)
                     /gene="yqcC"
                     /locus_tag="ACHKW5_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO7512186.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19086..19631)
                     /gene="syd"
                     /locus_tag="ACHKW5_002828"
     CDS             complement(19086..19631)
                     /gene="syd"
                     /locus_tag="ACHKW5_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO7512187.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19699..20547
                     /gene="queF"
                     /locus_tag="ACHKW5_002829"
     CDS             19699..20547
                     /gene="queF"
                     /locus_tag="ACHKW5_002829"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO7512188.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20659..22023
                     /gene="ppnN"
                     /locus_tag="ACHKW5_002830"
     CDS             20659..22023
                     /gene="ppnN"
                     /locus_tag="ACHKW5_002830"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO7512189.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22580..23869
                     /gene="sdaC"
                     /locus_tag="ACHKW5_002831"
     CDS             22580..23869
                     /gene="sdaC"
                     /locus_tag="ACHKW5_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO7512190.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23927..25294
                     /gene="sdaB"
                     /locus_tag="ACHKW5_002832"
     CDS             23927..25294
                     /gene="sdaB"
                     /locus_tag="ACHKW5_002832"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO7512191.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25406..26161
                     /gene="xni"
                     /locus_tag="ACHKW5_002833"
     CDS             25406..26161
                     /gene="xni"
                     /locus_tag="ACHKW5_002833"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO7512192.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26216..27364)
                     /gene="fucO"
                     /locus_tag="ACHKW5_002834"
     CDS             complement(26216..27364)
                     /gene="fucO"
                     /locus_tag="ACHKW5_002834"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO7512193.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27392..28039)
                     /gene="fucA"
                     /locus_tag="ACHKW5_002835"
     CDS             complement(27392..28039)
                     /gene="fucA"
                     /locus_tag="ACHKW5_002835"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO7512194.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6880 a         7524 c         6743 g         7106 t
ORIGIN      
        1 aaatctaaaa ttaccgatgc cgccgtcaat ttatttatcc agatttaatg agaaatttat
       61 aatgcgctat ttcatactaa tgttcacttt cgtatgttcc tttgttgcag cccaaccaac
      121 tattgtccca caattacagt aacaagttac ggatcttacg agtagcttaa actcacaaga
      181 aaagaaagaa ctgacgcaca agttagaatc tattttcaat aatacccaag tgcaaattgc
      241 tgtattaatt gttcccacaa ccaaagacga gacaattgaa caatatgcta caagagtttt
      301 tgacaattgg cgtttaggag atgccaaacg taatgatggg atactgatca ttgttgcctg
      361 gtcagatcgt actgtccgca tccaggtagg ttatgggctg gaagaaaagg taaccgatgc
      421 tctggctaga gatatcatcc gtagcaacat gatacccgca tttaaacaac aaaaatttgc
      481 tcagggatta gagctagcta taaacgcttt gaataaccaa ctcacttcac aacaccaata
      541 tccgactaat ccttcagaaa gtgaatcagc gtcttccagt gatcattatt actttgctat
      601 tttttgggta tttgcagtga tgttcttccc tttctggttt tttcatcaag gcagtaattt
      661 ttgtcgcgca tgtaaaagtg gagtctatat ttcagcaatc tatcttttag atttattcct
      721 gttctccgat aaaatttttt ccattgctgt attttccttc ttttttactt tcaccatatt
      781 tatggtcttt acctgtttat gtgtacttca gaaaagagca tcaggtaaaa gttatcattc
      841 agacaacagc ggttccggtg gaggctcaag tggcggcgga tttagcggcg gtggcggttc
      901 ttctggaggt ggcggcgcat ccggccgctg gtaactcaac cataaaaaat gccagcccgg
      961 aggctggcat ttttaaatca gataaagtca gtcttatgcc tggcctttga tctctttacg
     1021 accgttgtac ggtgcttttt cgcccagagc ttcttcgata cgaatcagct ggttgtattt
     1081 agcaacgcgg tcagaacggc tcatagaacc agttttgatc tggcctgcag cagtaccaac
     1141 agccaggtca gcgatggtag cgtcttcagt ttcgccagaa cggtgagaga taactgcagt
     1201 gtagccagca tctttcgcca tcttgattgc agccagagtt tcggtcagag aaccgatctg
     1261 gttgaatttg atcaggatgg agttagcgat acctttttcg ataccttctt tcaggatctt
     1321 ggtgttggtt acgaacaggt cgtcaccaac cagctggatt ttgtcgccca gaactttggt
     1381 ctggtatgcg aaaccgtccc agtcagattc gtccagaccg tcttcgatag aaacgatcgg
     1441 gtactgtttg gtcagttctt ccaggaagtg agtgaattct tcagaggtga acgctttgtt
     1501 gccttcgcca gccagaacgt atttaccgtc tttgtagaat tcagaagctg cgcagtccat
     1561 cgccagagtg atgtctttgc ccagttcata accagcagct ttaacagctt cagcgataac
     1621 agccagagct tcagcgttgg aacccaggtt cggcgcatag ccaccttcgt caccaacagc
     1681 agtgttcatg cctttcgctt tcagaacttt tgccaggtga tggaaaactt cagaacccat
     1741 gcggatggct tctttcacag ttttcgcgcc aaccggctga atcatgaatt cctggatatc
     1801 aacgttgttg tcagcgtgct caccaccgtt gatgatgttc atcatcggaa ccggcataga
     1861 gtatttgccc ggagtaccgt tcagttcagc gatgtgctcg tacagcggca tccctttagc
     1921 agctgcagca gctttggcgt tagccagaga tacagccagg attgcgttcg cgccgaattt
     1981 ggatttgttt tcggtgccgt ccaggtcgat catgatcttg tcaatgccag cctgatcttt
     2041 agcatctttg ccaatcagcg cctgagcgat cgggccgttt accgcagcaa cagctttggt
     2101 tacgccttta cccaggaaac gggatttgtc gccatcgcgc agttccagag cttcacggga
     2161 accagtagaa gcacctgacg gagcagctgc cataccgacg aaaccaccct ccagatgtac
     2221 ttcggcttca acagtcgggt taccacggga gtcgatgatt tcacgaccga tgatttttac
     2281 gattttggac attaggtttt cctcaagtca ctagttaaac tgaaactcca gacaaacaac
     2341 gcgtacccag ggtacgcgtt gccgttctaa cttttttact tacttcgcct gacgtttctg
     2401 gaactcgctg gcggctttca caaagcctgc aaacagcggg tgaccatcac gtggagtaga
     2461 agtaaactcc ggatggaact ggcaagccac gaaccacggg tgattcggaa cttcgatgat
     2521 ctcgaccaac tgatcatccc cggaacggcc cgcaacgcgc agacctgcat cttcaatctg
     2581 tttcaacagc atgttgttga cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc
     2641 attgtacagc tggcgaacca ggctatcgtc aaccaactgg cactgctgtg cgccgagacg
     2701 catggttccg ccgagatcgc tcttctcgct acgaacttca acgttgccgt tttcatcgcg
     2761 ccactcggta atcagcgcca caaccgggta cttacattct ggcacaaatt ccgtagagtt
     2821 ggcgttctcc atgttggcaa catggcgagc gtaatcaatt aacgccacct gcatacccag
     2881 gcaaatgccc agataaggaa tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc
     2941 ttctacgcca cgatagccaa aaccgccagg tacgaggatt gcgtccagac ctttaaggat
     3001 ttcaacgccg cgcgtttcaa catcttgtga atcgatcagt ttgatgttga cgctgacacg
     3061 attcttcagc ccaccgtgtt tcagtgcttc gatcactgat ttataagcat ccggcagttc
     3121 aatgtacttg ccgaccatac cgatggtgac ttcacttacc ggattcgctt cttcgaagat
     3181 aacctgttcc cattcggaca gattcgcttc cgggcagttt aagctgaatc gtttacaaat
     3241 ataatcgtcc agcccctgag atttcaacag gcccggaatt ttatagatgg aatcgacgtc
     3301 tttcagagaa ataaccgctt tttccggaac attacagaac aatgcaatct tcgcacgttc
     3361 gttcgccgga acagcgcgat ctgaacgaca aatcaggatg tcaggctgga taccgatgga
     3421 gagcagctct tttacagagt gctgagtcgg tttggttttg acttcaccag acgctgccat
     3481 gtacggcacc agcgtcaggt gcataaacag agtgtgctca cggccaattt caacagccat
     3541 ctggcgaatc gcttcgagga acggcaagga ttcgatatca cctactgtac cgccgatttc
     3601 taccagtact acgtcatgac cttcgccacc ttccagcacg cgctctttga ttgcgttagt
     3661 gatgtgcgga ataacctgca cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag
     3721 aacgtcagag tagatacgac ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat
     3781 gaaacgctcg tagtgcccca ggtccaggtc ggtttcagcg ccgtcttcag taacgaacac
     3841 ttccccgtgt tggattgggc tcatagtacc tggatcgacg ttgatgtacg gatccagttt
     3901 catgatggtc acattgaggc cacgggcttc aagaatggct gcgagggagg ctgcggcaat
     3961 gcctttaccc agagaggata cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct
     4021 gaacctgaga agttaggttg aaaagacgat ggaataacca ggacgggaaa gcagtatacc
     4081 cgaacatgac ctgtgccaca aactttcatt atccctcctc ttcgccagcg cactattgaa
     4141 atcaggagtg agaaaatagc ccctttgggg taaatgtttt tgacgcaaat caagcgcttg
     4201 tcatttaaaa aatgacacaa atggcgcttg accgcgtaat tcccttagag atcaatttcc
     4261 tgccgtttta cctgttgcca gacttcttcc attgtttcga ggtcaacacc tgtcatttcc
     4321 agtccacgcg cggcaacaat acgctccact tcgcgaaaac gacgctcaaa tttttcgttc
     4381 gctttttgca atgcgatttc cgcttttgtc cctaaatgac gagccagatt gaccgtggca
     4441 aacagcaggt cccccatttc ctcctccagt ttagcctggt cgacaacagc ctgccgcgct
     4501 tcgtacatca cctcgtcgat ctcttcgtag actttatcga ctaccggacc aagcgtcgtc
     4561 caatcgaagc caacgttggc gcaacgtttc tggatttttt gcgcacgcat taaagccggt
     4621 aaactacgag gaatatcgtc cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt
     4681 ttgatttgct cccaacgggc aagcacttca ctactgtttt cggcagaact atcagcaaaa
     4741 acatgcggat ggcgacgctc taatttatcg ctaatagcag cgcaaatatc attaaagtca
     4801 aagcgccctt cttcctgagc catttgcgcg taaaacacca cctggaatag cagatcgccc
     4861 agttcaccgc gaagatcgtc aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt
     4921 tcttcaaggg tgtaaggcgc aatggtggca aatgtctgct ctttatccca cgggcagccg
     4981 ttttccggat cgcgcaggcg ctgcataata gtgagcaaac ggtcgatttg attcattgaa
     5041 ttgtcctgaa aattgcgggt ctgtcaggtg gaaacctgtg accagaatag aagtgagtta
     5101 gtaacactac ccaatcagta cgttaatttt ggctttaatg agttgtaatt cctctggggc
     5161 aactgttcct ttcttcgttg ctcctcttgc ccgccaggcg atacttttta cctgatcagc
     5221 taacgctacg ccatcacgtt cctgaccgga taaaacaact tcgaacggat atccttttga
     5281 ttgcgttgta taaggaacac acagacacat acctgttttg ttgttgtaca tgaaaggact
     5341 caggacaaca gctggacgat gtccagcttg ctcgctacct tttgtcgggt caaaatcaac
     5401 ccaaatcaga tcgcccatat cgggtacgta tcggcttacc attaccagac ttccttatct
     5461 ttcggctctc cccagtcgat attctcgtgg aggttttccg gcgtgatgtc gttgaccagt
     5521 tcagcaagcg taaatacggg ctctttacgc actggctcaa taattaattt gccatccacc
     5581 aggtcaatct tcacttcatc atcaatattc agattgagcg cctgcattaa cgtagccggg
     5641 atccgcaccg ccggtgaatt tccccaacgc tttacgctac tgtggatcat aaccctttcc
     5701 tcaaaccgct atcatatgta gatacagtat atatcaatct acattgtaga tacgagcaaa
     5761 tttcggccta actcccgtgc aaccgacgcg cgtcgataac atccggcacc tggttgagtt
     5821 tacccagcac gcgccccagc acttgcaggt tgtaaatctc aatggtcatg tcgatggtcg
     5881 ccagttgctg tttggtgtcg ctacggctgg caacgccaag cacgttcacc ttctcgttgg
     5941 cgagaatggt cgtgatatca cgtaacaacc cactacgatc attagccacc acacggacca
     6001 ccagcgaata tccggcggag tagctctcac cccataccgc gtcaacaatg cgttctggcg
     6061 catgggagcg cagttccgcc agttgttcgc aatcggcgcg gtgtactgaa ataccgcgcc
     6121 cctgggtaat gaagccgaca atctcatctc caggaatcgg ctggcagcag cgcgcgatgt
     6181 ggtgcatcag gttgccaaca ccttcgacca ccacgcgacc gttatcttta ctgcggtttt
     6241 gcggcgtgta gcttttttgc tgaagttgct tcagcgcggc ggcgtcctgc tcttcggcac
     6301 tcggcttatt aaattgcgat tgcaggaagt tcaccatctg attgagacgg atatccccgc
     6361 caccaatcgc cgccagcaac tcgtcgacat cattaaagtt gtaacgcggc agcagatgtt
     6421 tttctgcttc tttcaggctg atccccagat gttccagctc gtcgtcaagg atttgccgcc
     6481 cagccagaat gtttttgtca cggtcctgtt tacggaacca ggcgtgaatt ttcgaacgcc
     6541 cacggctggt tgtgacgtaa ccgaggtttg ggtttaacca gtcacggctg gggttcggct
     6601 gtttctgggt gataatttca atctggtcgc ccatctgcag ctggtaggtg aacggcacaa
     6661 tgcgcccgcc aatttttgcc ccgatgcagc ggtgtccgac atcactgtgg atgtggtaag
     6721 cgaagtccag cggcgttgat cccgcaggca aatcaacgac atcacctttc ggcgtaaaga
     6781 catacacccg gtcgtcaaag acctggctgc gtacttcgtc gagcatttcg ccggaatcag
     6841 ccatctcttc ctgccacgca atcagtttac gcagccaggc aatccggtct tcatgtcccg
     6901 aacgtgcgcc gccagctgcc gcgccctctt tatatttcca gtgcgcagca acacccaact
     6961 ctgcatcttc atgcatctgt ttggtgcgga tttggatctc aacggttttg ccacctggcc
     7021 ccagaaccac ggtatgaata gactgataac cgtttggttt cgggttagcg acataatcgt
     7081 caaactcatc cggcaggtgg cgatagtgag tgtgcactat ccccagtgcg gcataacaat
     7141 cctgtaaacg ctcggcgaca atacgtaccg cacgcacatc aaacagctca tcgaaggcga
     7201 ggttcttttt ctgcattttg cgccagatac tgtagatgtg tttcggacga ccatacactt
     7261 cagctttaac gccttcagct ttcatctcag cgcgcagatg gccgacaaac tcttcgatat
     7321 agtgttcgcg gtcgagacgg cgttcatgca gcagttttgc gatgcgtttg tattcggtcg
     7381 ggtggagata acggaagcag taatcttcca gctcccattt cagttgccca atcccaagac
     7441 ggttcgccaa cggcgcgtag atattggtgc actcttttgc cgccagtacg cgttcatctt
     7501 ccggcgcatc ttttacttcg cgcagatgag caatacgctc cgccagtttg atgaccacgc
     7561 agcgaaaatc atcgaccatc gccaataaca tccggcgaac gttatcgacc tgttcggagg
     7621 aaacagaatc agtgtgcgtc gctttcagct ggcggatcgc cgccatatca cgcacgccgt
     7681 gaataaggtt aacgaccgac ttaccgacgc tctcacgcag cacatcttcg ctgactacgt
     7741 tggcatcagc cagagggaac agcagcgccg cccgcagcgt gtcaatgtcc atacttaatg
     7801 tcgagaggat ctccaccatc tcaacaccac gccacaataa cagactggca tccggatgcc
     7861 cctgcgtctg ttgcagacaa tacgcccagg tttcggctaa gcactcacac gacttctggc
     7921 tggtaatacc cagacttgcg atccattttt ccggatcaaa ttcaccagcc ttattgatat
     7981 gtgcacttct taccgcaacc attgtcctct cctttaggga ccagacctgc cgaaatcggc
     8041 aaatcgtaac tatttaacgc gcgagaaaag taccatcgat tccagatgtc ccgtgtgtgg
     8101 gaacatatcc agcatcgcca gtcgcgcaat ggtatatcct gcttttaata acgcttcgct
     8161 atcccgagcc agcgttgcag ggttacagga tacataaact atacgaatag gttccagttt
     8221 tataatttgc tgcataacac ctgcggcacc tgctcgcgcc gggtccagca acactttatc
     8281 gaagccgttt ttcgcccacg gctgctttgt gacatcttct tcaagatttt cgtgataaaa
     8341 cgtcacattc tgtaaaccat taagacgcgc attctgctgg cctttttcca ccagcgccgg
     8401 aacaccttct acaccgacca cactggctgc ttgtttcgcc aatggcagtg taaagttgcc
     8461 cataccgcag aacagatcca gtacgcgatc ttcaggttgc acatccagcc attccaacgc
     8521 acgcgctacc attttttggt tcacacccgc attgacctga ataaaatcgc gcgggctaaa
     8581 agttaagcgc aacccgtttg agtcatacca gggcatctca ccagagacgg tttcgagtat
     8641 ctcactatcg ggggcgagat acagatccag gccttcagaa tgcgaaaagc gttccagttt
     8701 ttcgcgatct gccgaactta gcggtgcggt atggcgcaaa atcatcagcg tgccgctggt
     8761 tgcctgtacc agttcaacat gaccaagatg gcgcataact tgtaagctgc ccaggcatgc
     8821 cctgactttg ggcagcaatg cttcaagttg gggcactaaa atggggcatt gtttaacgtc
     8881 gacaatgtca ctggagcccg ctttgcgaaa ccccatctga agttgctgtg ttttcggtaa
     8941 gtagttcaga cttaaacgcg cgcggcggcg atagccccag ggaacatcgg cgatcacttc
     9001 agagacatcg tgtttcatta atcgggcgag tgccgcactt ttgcttcgct gctgtaaatc
     9061 cacgctggcg tgttgttgct gacagccgcc gcatacgcca aaatgaggac agcgtggtgt
     9121 ttcgcgttcc gggctatcgc ttaaccggcg tacgacttta gcgcgggcat actgtttttt
     9181 atcttcagta acagcaactt ccgcgttttc ctgcggcaat aatccgggga taaataacgt
     9241 tttgccgtta tgtcgcgcca cgccctgacc aaaagagtcg aggtcgttga ctgaaacggt
     9301 tatgatctga cgcgtcgtcg tgcgtcgttt tgcagagtag aattgcgcca ttggcgagac
     9361 tttctcaatt taacagtgtg accttaattg tcccataacg gaactccatg accaactaca
     9421 gcctgcgcgc acgcatgatg attctgatcc tggcaccgac cgtccttatt ggtttattgc
     9481 tgagtatctt tttcgtcgtg catcgctata acgacttgca gcgtcaactg gaagatgccg
     9541 gtgccagcat tattgagccg cttgcagttt ctactgaata tggcatgagc ctgcaaaatc
     9601 gcgaatctat cggtcagtta ataagcgtac tgcatcgtcg ccattccgat attgttcgcg
     9661 cgatttcggt ttatgatgaa aataaccgac tctttgtcac ctccaatttt catcttgatc
     9721 cctcatcaat gcagctcggc agcaacgtgc cgtttcctcg ccagctcact gtcactcgtg
     9781 acggcgatat tatgatcctc cgcacgccga ttatttctga gagttactcc cccgacgaat
     9841 cgcccagtag cgatgccaaa aatagtcaaa atatgttggg atatattgcg ctggagctgg
     9901 atcttaaatc ggttcgcttg cagcaatata aagagatctt tatttccagc gtgatgatgc
     9961 tgttttgtat cggtattgcg cttatttttg gctggcgctt aatgcgcgat gtaaccggtc
    10021 cgattcgcaa catggtgaat accgtcgacc gcatccgtcg cgggcaactc gacagccggg
    10081 tggaaggatt tatgctcggc gagctggata tgctgaaaaa cggtatcaac tcgatggcaa
    10141 tgtcgctggc tgcttatcac gaagagatgc agcacaatat cgaccaggcg acgtccgatc
    10201 tgcgtgaaac gctggagcag atggaaattc agaacgttga gttagatctg gcgaaaaagc
    10261 gcgcccagga agcggcgcgt attaaatccg agtttctggc aaatatgtca cacgagctgc
    10321 gtacaccact gaatggtgtt attggcttta cccgcctgac gctgaaaaca gaattaacac
    10381 caacgcagcg cgatcacctg aatacgattg aacgttcggc aaataatttg ctggcaatta
    10441 ttaatgatgt tctcgacttc tcgaaactgg aagcaggtaa gctgattctg gaaagtattc
    10501 cattcccact acgcagcacg ctggatgaag tcgttactct gctggcacat tcttctcacg
    10561 ataaagggtt ggaactgacg ctcaatatta aaagcgacgt gcctgataac gtgatcggcg
    10621 acccactgcg attacagcaa atcatcacta acctggtggg gaatgcaatt aaattcaccg
    10681 agaatggcaa cattgatatt ctggtagaaa aacgtgcgct gagtaatacc aaagtgcaga
    10741 ttgaagtgca gattcgggat accggcattg gtattcctga acgcgatcaa tcgcgcttat
    10801 tccaggcctt ccgacaggct gatgccagta tttcccgccg tcatggtggc accggtctgg
    10861 ggctggtgat tacacaaaaa ctggttaatg aaatgggcgg cgatatttcg ttccatagcc
    10921 agccgaatcg cggttcaact ttctggttcc acattaatct cgatctgaac ccgaacatta
    10981 ttatcgaagg gccatccacc cagtgcctcg ccggtaagcg cctggcctat gtcgaaccaa
    11041 actccgcagc agcgcaatgc acgctggata ttttaagtga aacgccgctg gaagtggttt
    11101 atagcccaac gttctccgcg ctgcctcccg cgcattacga catgatgttg ttaggcatcg
    11161 cggtgacctt ccgcgagccg ctaacaatgc aacatgagcg attagcgaaa gcggtatcga
    11221 tgaccgattt cctgatgctg gcacttcctt gccatgcaca agtcaatgca gaaaaactca
    11281 agcaagatgg tatcggcgcg tgtctgctga aaccattaac acctacgcgc ctgttgcctg
    11341 ccctgacgga attttgtcat cacaaacaaa acacgctttt gcctgtaacc gatgaaagca
    11401 agctggcaat gacagtcatg gcagttgatg acaaccccgc taacctgaaa cttatcggcg
    11461 cattgctgga agatatggtg caacatgtgg aactttgcga tagcgggcat caggcggttg
    11521 aacgggcgaa acagatgccg ttcgatttga tcttaatgga tattcaaatg cctgacatgg
    11581 atggcattcg ggcctgcgag ctcatccacc agctccccca tcagcaacaa acgccggtta
    11641 tcgcggtaac ggcgcatgca atggccgggc aaaaagagaa gctgcttggc gcagggatga
    11701 gcgattatct ggcgaaaccg attgaagaag agcgattgca taatttgttg ttgcgctaca
    11761 agcctggcag cggtatttcc tctcgcgtcg tgacgcccga agtcaacgaa attgtggtga
    11821 acccgaatgc gaccctcgac tggcaactgg cactacgcca ggcagcagga aaaaccgatt
    11881 tagcgcgcga tatgctgcaa atgttactcg atttcctgcc tgaagttcgc aacaaagttg
    11941 aggaacagct ggttggagaa aacccggaag gcctggttga tttgattcat aaactgcatg
    12001 gcagttgcgg ctatagcggt gtgccgcgta tgaagaatct ctgccaactg atcgaacaac
    12061 agctacgtag cggtactaaa gaagaagatt tggagccgga gctgctggaa ctgttggacg
    12121 agatggataa tgtagcgcgc gaagccagca aaattctcgg gtaacggacg tcttgtcgag
    12181 tagcaatcgg ctttttatga cgctggtttc agattggtga caaagtgcct gtgtttatgc
    12241 cggatgcggc gttaacgcct tatccggcct acaaaaccaa ttaaattcaa tgaattaaaa
    12301 aatgatgtag acctgataca tagcacaaca ggctattttg cgtttagcat cagtctcaag
    12361 ccggctccag atagagccgg ttttggtttt ctgtcttaac gcaccatgca cgggcgcttg
    12421 ttatcgaacg tccagccagg aatcagatac tgcattccca tcgcatcgtc acgcgcgcca
    12481 agcccgtgtt tctgatacag ctcatgggct ttcatcactt gatccatatc gatttctaca
    12541 cccagccccg gtttttctgg cacctgtacc agcccgcctt tgatctcaaa cggttctttg
    12601 gtcaggcgct gattaccttc ctgccagatc cagtgcgtat caatagcagt aattttaccc
    12661 ggtgcagcgg cggcaacatg ggtaaacatc gccagggaaa tatcgaagtg gttgtttgag
    12721 tgtgaacccc aggtcaggcc aaattcatgg cacatttgcg ccacacgtac cgaaccttgc
    12781 atcgtccaga aatgcggatc cgccagcggg atatcaacgg attgcaggga gagcgtatgg
    12841 cccatctgcc gccagtcggt ggcgatcata ttggttgcgg tcggcaggcc agttgcgcga
    12901 cggaactctg ccatcacttc acgcccggag aaaccttgct ccgcaccaca cggatcttct
    12961 gcataagcca gcgacccttt caggtattta ccaattttaa tcgcttcgtt cagcgaccag
    13021 gcaccgttag gatcgagcgt aatacgcgcc tgcgggaagc gttgcgccag tgccacaata
    13081 gactcggcct cttcttcccc ggccagtaca ccgcctttca gtttgaaatc gttgaagcca
    13141 tatttttcat acgccgcttc cgccaggcgc accaccgcat ccggcgtcat cgcttcttca
    13201 tgacgcaggc gataccagtc gcatgagtca tccggctggc tttgatacgg cagcggtgtg
    13261 gctttgcgat caccgacgaa gaacagataa ccgagcattt cgacttcgct acgctgttga
    13321 ccatcgccca gcagggatgc cacgtttacc cccagatgct gccccagcag atccagcatt
    13381 gccgcttcta tcccggtaac tacatgaata gtggtacgca aatcaaatgt ctgcaaaccg
    13441 cgcccaccag catcacgatc ggcaaaagta ttacgcacca gcgtcagaac gtttttgtat
    13501 tcacccagcg ttttacctac caccagcgga atcgcatctt ccagcgtttt acggattttc
    13561 tcgccgccgg gaatttcccc tacgccagtg tgaccagaat tatctttgat aatcacaata
    13621 ttacgcgtaa agaacggtgc gtgtgcacca ctcagattca tcagcatact gtcatgaccc
    13681 gccaccggga tgacctgcat ttcagtaaca acaggcgtcg taaattgaga actcatattt
    13741 atgtcctttt tcagaatcaa tgacggccga aaacgggacg tttacggtca aaggtccagc
    13801 cggggatcag gtactgcatc ggacctgcgt cgttacgcgc accgacaggc agacgtttat
    13861 aggcctcatg tgccttttgt acctgttccc agtccagttc cacgcccaga ccgggcgcat
    13921 caggaacggc aatttttccg tttttaatct ccagcggatt tttggtcagg cgacaatcgc
    13981 cctcctgcca aatccagtgg gtatcgatag cggtaggatt acccggtgcc gccgcgccca
    14041 catgggtaaa catcgccaga gagatatcga aatggttatt agaatggcag ccccaggtca
    14101 gcccccagtc gtcgcaaagc tgcgccacac ggactgcacc ggaaagcgtc cagaagtgcg
    14161 gatcggcaag tggaatatct accgcattga gcatcaccgc atgacccatt tcgcgccagt
    14221 tggtggcgat catgttagtc gcgacgggca agccggtcgc ccgccgaaac tccgccataa
    14281 cttcacgacc ggagaaaccc tgttctgcgc cgcatggatc ttcggcatag gtaagaacat
    14341 cattcagccc tttgcataga gaaatggctt catcaagtag ccaggcaccg ttgggatcaa
    14401 cggtaatccg cgcatcaggg aagcgtttct tcaatgcacg aacagtgtcg atttcttgct
    14461 cgccaggtaa caccccgccc ttaagtttga aatctttaaa gccatagcga tcctgtgagg
    14521 cttccgccag acgcacaacg gcttcgctgt tcatcgcttt ctgatggcgc aactgatacc
    14581 actcatggtt gcccggcgta ttttccagat aaggaagatc ggttttggtc cgatcaccga
    14641 tataaaacag ataaccgagg acggtaatag tctcgcgttg cttgcctggc cccaacagtt
    14701 cgcagaccgg aacattcagc gccttaccta gcaggtcaag caaggcggct tccagcgccg
    14761 ccacggcatt aacgcgcaat tcaaaagtcc aggcaccttt gccgaaggta tcaaaatcgg
    14821 ctgcctgatt acctttatgc acctgctgaa ccactttatt caggcgcgca acttcctggc
    14881 ccagaaccat cggaatagca tcgaccagtg tttgataaat cacctctccg cccggcgctt
    14941 caccaatgcc ggtatgcccg gcgttatcgg tgagtaccac aatattgcgg gtgaaatatg
    15001 cgttatgtgc cccaccaata ttaagcaaca tgctatcgtg cccggcgacc ggaatgactt
    15061 tcatatcagt aataacagga ctggattgtg tcgtcatctt attgccccgc gacaggtttc
    15121 agctcgatac gcttgatatc gcccaccagc accaggtagc tcagtaccgc gattaaggca
    15181 tgaacaccaa cataaatcag cgccccatta aaggagccag tcgtgccaac gatataacca
    15241 attgcgattg gcgtgacgat gccagaaatg ttaccgaaca tgttgaacag gccaccggaa
    15301 agaccgctga tctcttttgg cgcggtatct gccattactg cccaacccag cgccccgatg
    15361 cccttaccga agaaggccag cgccataaag ccgatgatca tccactcaac gttgacgtag
    15421 ttgcagaaca ccatcaccat cgacagcaac atgcccatta cgatcggtgt tttacgcgca
    15481 atgttcagcg atcccgtgcg gcgcatcagc cagtcggaaa taatcccacc cagcacaccg
    15541 ccgataaaac cgcaaaccgc cggaacggaa gccacaaagc ccgctttcag aatcgacatc
    15601 ccacgcgcct gcaccagata aaccggaaac caggtaatga aaaagtaagt cagggcgttg
    15661 atacagtact gaccgatata aacgccgatc atcatccgtg acccgagcag ttgtttgatc
    15721 tgcccccact tcacgctgaa cggcacttta actttggtgt tttgctgatc catattgatc
    15781 agcgcgccac ccgcggcgat gtactccagc tctttctgat ttacccccgg atgttgattt
    15841 ggctcgtgga tgactttcaa ccagataaag ctgatgacaa tccccagacc gcccataaag
    15901 aagaagacgt gtgaccagcc cacttcatgc gtcagccagc ccataatcgg cgcgaagatc
    15961 accgttgcga agtattgagc ggagttaaaa atcgacaccg ccgttcccct ttcctgcgcc
    16021 ggaaaccagg ccgcaacaat gcgactgttg ccggggaaag atggcgcttc agcaagcccg
    16081 accaggaagc gcagagtaaa cagggcaacg ataatgccga atccactaaa gatatcgacg
    16141 aagccttgca gcaaggtaaa catcgaccag ataaagatcg accagaagta gacgcgtttt
    16201 gaaccaaaac gatccagcaa ccaaccacca gggatctgcc cgataacata agcccatgag
    16261 aaggcagaga acacatagcc cattcccacg ggatcaaggc cgatatcttt ggccatttcc
    16321 gaaccggcaa tagaaagcgt agcgcggtcg ccgtagttga aggatgtgac gataaacaac
    16381 atcaccacta tccagtaacg agcattcgtg cgtttttcca cactgctcgc agcctgactt
    16441 aaagaactca ttgttgcact cctgaaaatt cgcgttagcc acgctcactc tggactgcga
    16501 catagccagg aaatcagagg tgacgtaggg tgttttttgc cgtttttata ggtcgttcgc
    16561 cgaatacggc gcgtgtttat atctggcaat agcagtataa aaagcgcgcc atagcggctc
    16621 accgtgcaac aacacaacat taatgcgttc aatgaggccc gattttggca ttagccctgg
    16681 acggtggaat ccacttcacg gaaatgaaaa caagaacaag aaaggaaggg ttaaaacgaa
    16741 gaaataaaga agagtatgaa atggatcgct tgactccagg caaacgccag taaaaatccg
    16801 cgctatgaag cagtttttac tggcatttgc ctgaaaatat tcgattcagc accgctaaaa
    16861 cgacatttac cgctcgctga acatatcagg acaacagcgt gccccactgt tcgacccacg
    16921 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16981 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcagaaat
    17041 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    17101 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    17161 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    17221 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    17281 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    17341 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    17401 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    17461 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    17521 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    17581 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    17641 gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    17701 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    17761 tgctggctgc gagcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17821 caccagtgga taatccagaa ctgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17881 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17941 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcgg taaaaacgtg
    18001 ctggccaatc tgatcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    18061 gctgcggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    18121 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccagctca
    18181 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    18241 acggcaaaag cgcccggtaa cggctgtttg ttatctaaca aatcgtgcat tcgcgggatc
    18301 agcacccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    18361 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    18421 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    18481 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagcgctc
    18541 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    18601 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    18661 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    18721 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18781 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18841 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18901 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18961 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    19021 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    19081 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaatgc
    19141 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    19201 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    19261 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    19321 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    19381 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    19441 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    19501 gttgaaataa tgcacggtga aggaacaccg tacagttcct cacttaacgg ccagctttta
    19561 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    19621 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    19681 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    19741 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19801 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19861 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19921 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaaa
    19981 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    20041 ctggagcgcg acttaagcac ttgcgcccag ggtaaggtta gcgtggcgtt atatcgtctt
    20101 gatgaactgg aaggccagcc gataggtcat ttcaatggca cctgcattga tgaccaggat
    20161 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    20221 aaagtagtgg aagagacgct tgtcagccac ctgctgaaat caaactgcct gatcacccat
    20281 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    20341 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    20401 cgcatcttta atgacctgtt acgcttctgc cagccagaaa aattgagcgt ttacgcacgt
    20461 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    20521 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    20581 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    20641 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    20701 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    20761 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20821 ctgtctcgtt ttgaaaattt cgatattaac gtcttgcgcc gtgaacgcgg cgtaaagctg
    20881 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20941 gccaacttgt tcgcggtcct gcgtgacatt ctcttcgttt acgggcaaat ccataacacc
    21001 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    21061 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    21121 ggcggtcact caattaacga aaatgagtat ttgtatgccc gtcgcgtcgg aaaccagctg
    21181 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccgg gagcgatgga agcgccgatg
    21241 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    21301 acagagccgt cgattatcgc cgctgaaccg cctaacccgc tggtcaacga attgatcatc
    21361 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    21421 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    21481 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    21541 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    21601 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    21661 ctggtgaaag aaaatcgccg cgacactggt gacgcttaca gctttaactg gtcaatgcgc
    21721 attgcaccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21781 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21841 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21901 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21961 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    22021 taatctccgc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacttactc
    22081 atcaactcat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga caaatatcat
    22141 cacagttaat atgtcataca atttatgttg caacgcaaac gtttccctat tttcataaac
    22201 cgtattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    22261 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa
    22321 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    22381 tcttccaagt agattattgt atttgagatc aagatcactg atagatacat aacttgtgtg
    22441 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    22501 tttcattttt acggtcaggc accttcccgg gctgaactgg ctaaaagctg aattatttgc
    22561 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    22621 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    22681 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    22741 catcatggct atccttgcgt tcccgatgac gttttttgct caccgcggcc tgactcgctt
    22801 cgtactgtct ggtaaaaacc cgggcgaaga catcaccgag gttgtagaag aacactttgg
    22861 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    22921 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22981 gacgccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    23041 tcgctttggt gagcagatga tcgttaaagc gatgagtatt ctggtattcc cgtttgttgg
    23101 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    23161 gctgtctctg gacactgcat ctgcaaccgg gaacggtctg tggatgaccc tgtggctggc
    23221 aattccggta atggtgttct cgttcaacca ctctccgatc atctcttctt tcgccgttgc
    23281 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc ttgcattcgc
    23341 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgcgtac tgagcctgac
    23401 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    23461 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    23521 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    23581 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    23641 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    23701 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    23761 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    23821 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    23881 ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta tttcctatga ttagcgtatt
    23941 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    24001 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    24061 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    24121 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    24181 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    24241 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    24301 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    24361 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    24421 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    24481 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    24541 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    24601 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    24661 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    24721 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    24781 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agtttatccg
    24841 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    24901 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24961 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    25021 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    25081 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    25141 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    25201 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    25261 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    25321 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    25381 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    25441 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gtcagcatgc
    25501 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    25561 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    25621 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    25681 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    25741 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    25801 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    25861 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    25921 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25981 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    26041 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    26101 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    26161 acggcgagcc ggatacgccg caaacgtcgt atccggcatt atcacatcag cgcatttacc
    26221 aggcggtatg gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg
    26281 tacaaacatc atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtaccccaa
    26341 catcacgcaa atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag
    26401 cggcattacg cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga
    26461 tatcgcggta cttctcaccg gtaaagtcag cgttatagcg catgacatgc ggtagcagga
    26521 tggcgtttgc aacaccgtgt ggagtgttat aaaacgcgcc cagtggatgc gccataccat
    26581 gcaccaaccc taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca
    26641 tttcttctcc ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt
    26701 caatcgcttt aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct
    26761 caatagcatg agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat
    26821 ccatcatgtc agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa
    26881 acttgcgccg tttttcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtgc
    26941 ctgctgtggt ggggattgcc agaatcggta cactgggttt attggtcggg gaaagtcctt
    27001 ccaggctacg cacatcggca aactccgggt tgttgctgat aatgccaatc gctttacaag
    27061 tatcctgtgg agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata
    27121 caccgagccc ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg
    27181 cccatgccag ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca
    27241 ccagcgtttt atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg
    27301 ttaaagcccc aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag
    27361 ccatcatcct tctccttgtt gctttacgaa attactcttc aattcgtaac ccataggttt
    27421 tgaatttctc cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa
    27481 tcgccagggt cgtcaggtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg
    27541 ctttttccag attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac
    27601 gatttttgag agcctgcgca acatgttcag aaagttcacg tgttccaaag gtcgcataag
    27661 gcgcgcaagg aatagaatta ccgccagccg ccgcaatcat gtagtgaata gcggggatcg
    27721 atcggttaag aatggaaact gccgtgcaat gaacggcatg attgtgaaca accgcgttgg
    27781 catccggtct gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc
    27841 cttcctcatg tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat
    27901 atggaatgcc tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg
    27961 ctgtcccctg gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag
    28021 caagtttatt tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt
    28081 tcctttcaca ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaaccag
    28141 aaagaaattc acaagcttat attttgtgac ctggttcaac taatcacagt aaataactgc
    28201 aagttctctt tttataaccc cattaaaaat gaccgctctt aaaaatattt atc
//
LOCUS       DAZFYC010000061        27963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFYC010000061 DAZFYC010000000
VERSION     DAZFYC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.061"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..358
                     /locus_tag="ACHKW5_002836"
     CDS             <1..358
                     /locus_tag="ACHKW5_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512195.1"
                     /translation="CLSQPFYSCSIVMVNTCKVSINISTVNNSTHYDGKVRDNFHLDI
                     GERKPIVYFIYTGRESDVFNKNPVNIVSELSILISDGNNSKTLSGYHISSLLKKSSSS
                     MESDFSFEINDPSICP"
     gene            434..2194
                     /gene="tssF"
                     /locus_tag="ACHKW5_002837"
     CDS             434..2194
                     /gene="tssF"
                     /locus_tag="ACHKW5_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7512196.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGSLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCFYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPFYLNADSPVACALHRALTLGTQQFWLRLPGQDRRTLDAHFS
                     PLGFEDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLELVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDADRMLEDETLSLSLTGTNGHLPRKALQSTLLDRPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            2158..3237
                     /gene="tssG"
                     /locus_tag="ACHKW5_002838"
     CDS             2158..3237
                     /gene="tssG"
                     /locus_tag="ACHKW5_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7512197.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQFLEQSQQNAPLGST
                     DNPATDAVRFRPHPGMGFPVSELKNVERDAGNPNVPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAMDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPALSIRNRESMTHVSYSF"
     gene            3218..3754
                     /gene="tssJ"
                     /locus_tag="ACHKW5_002839"
     CDS             3218..3754
                     /gene="tssJ"
                     /locus_tag="ACHKW5_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7512198.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            3758..4186
                     /gene="tssE"
                     /locus_tag="ACHKW5_002840"
     CDS             3758..4186
                     /gene="tssE"
                     /locus_tag="ACHKW5_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7512199.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMVAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     YLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            4186..5562
                     /locus_tag="ACHKW5_002841"
     CDS             4186..5562
                     /locus_tag="ACHKW5_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7512200.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     RVEKLSLALFRQNGVELQTQVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMKQPEKSEESPQPVFATRPAQQNDK
                     DASPSAPSPEISWQRTWPIFMAGMVVMACLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSTEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            5627..6175
                     /locus_tag="ACHKW5_002842"
     CDS             5627..6175
                     /locus_tag="ACHKW5_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HFO7512201.1"
                     /translation="MYKKERNRPFETREEVLAYHKREKIPCLECGKLLIFLPRHIWFV
                     HGIRADEYRVKWNIPKHIALAGISYLEKQSEYMKARIAKGKLDPAEQIAMMRIKGRQL
                     KSDPDWRNRSGSILKKQYARRDILKNRVWERSPSIKSVSVELKTEAIRRMKNRKVVGE
                     KVQDIADDLNVSISRLYHWLKM"
     gene            complement(6521..7774)
                     /gene="amiC"
                     /locus_tag="ACHKW5_002843"
     CDS             complement(6521..7774)
                     /gene="amiC"
                     /locus_tag="ACHKW5_002843"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO7512202.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            8006..9337
                     /gene="argA"
                     /locus_tag="ACHKW5_002844"
     CDS             8006..9337
                     /gene="argA"
                     /locus_tag="ACHKW5_002844"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO7512203.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(9399..11225)
                     /gene="recD"
                     /locus_tag="ACHKW5_002845"
     CDS             complement(9399..11225)
                     /gene="recD"
                     /locus_tag="ACHKW5_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO7512204.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKCKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(11225..14767)
                     /gene="recB"
                     /locus_tag="ACHKW5_002846"
     CDS             complement(11225..14767)
                     /gene="recB"
                     /locus_tag="ACHKW5_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO7512205.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSVGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(14760..17648)
                     /gene="ptrA"
                     /locus_tag="ACHKW5_002847"
     CDS             complement(14760..17648)
                     /gene="ptrA"
                     /locus_tag="ACHKW5_002847"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO7512206.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGAKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(17824..21192)
                     /gene="recC"
                     /locus_tag="ACHKW5_002848"
     CDS             complement(17824..21192)
                     /gene="recC"
                     /locus_tag="ACHKW5_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO7512207.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCHEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(21205..21528)
                     /locus_tag="ACHKW5_002849"
     CDS             complement(21205..21528)
                     /locus_tag="ACHKW5_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO7512208.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(21513..21920)
                     /locus_tag="ACHKW5_002850"
     CDS             complement(21513..21920)
                     /locus_tag="ACHKW5_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO7512209.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(21917..22480)
                     /locus_tag="ACHKW5_002851"
     CDS             complement(21917..22480)
                     /locus_tag="ACHKW5_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7512210.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(22471..22941)
                     /locus_tag="ACHKW5_002852"
     CDS             complement(22471..22941)
                     /locus_tag="ACHKW5_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7512211.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(23126..23920)
                     /gene="thyA"
                     /locus_tag="ACHKW5_002853"
     CDS             complement(23126..23920)
                     /gene="thyA"
                     /locus_tag="ACHKW5_002853"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO7512212.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(23927..24802)
                     /gene="lgt"
                     /locus_tag="ACHKW5_002854"
     CDS             complement(23927..24802)
                     /gene="lgt"
                     /locus_tag="ACHKW5_002854"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO7512213.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(24953..27199)
                     /gene="ptsP"
                     /locus_tag="ACHKW5_002855"
     CDS             complement(24953..27199)
                     /gene="ptsP"
                     /locus_tag="ACHKW5_002855"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7512214.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(27212..27742)
                     /gene="rppH"
                     /locus_tag="ACHKW5_002856"
     CDS             complement(27212..27742)
                     /gene="rppH"
                     /locus_tag="ACHKW5_002856"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO7512215.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         6673 a         7654 c         7103 g         6533 t
ORIGIN      
        1 atgtttatct caaccattct attcttgttc cattgtcatg gtaaatacat gcaaagtatc
       61 tattaacatt tcgacagtca ataattcaac acattatgat ggtaaggtaa gagataattt
      121 tcatcttgat atcggagagc ggaagccaat tgtatacttt atttacacag gtagagagtc
      181 agatgttttt aataaaaacc ctgtaaatat tgtttctgaa cttagtattt tgatatctga
      241 tggtaacaat tctaaaactt tatctggata tcatatctcc tctttattga aaaaatcctc
      301 atctagtatg gagtccgatt tttcttttga aataaatgat ccatctatat gtccttagga
      361 ttctgaaaat aaaaaattct aatctcacta ctcgttggta ttaatttcaa taaaaggaaa
      421 gataaatcct ccgatggacg acttaaccca acgctactac gaagccgaaa tgcgctacct
      481 gcgtgaagcg gggaaagaat ttgcgcaggc ttaccccgac cgcgccgcga tgctcaatct
      541 tgataaaccc ggcgcacgcg atccctatgt ggaacgcctg ttcgaaggct ttgctttttt
      601 aatgggcagc ctgcgcgaga agctggacga cgatctgccg gagctgactg aagggctggt
      661 cagcctgctg tggcctcact acctgcgaac tatcccgtca ctttccgtgg ttgaactgtc
      721 gacagatcac cggcagatga agcagagcga aacgctgagc gattttcagg tattgtcgcg
      781 ccctgttggc gagcgcagaa cccgctgttt ctacagcgcc acgcgggata tcacgctgca
      841 tccgctggcg ttgccggacg tgtcattgca atatgaaccg gacggacgtt cggttattcg
      901 tctgcgcttt gaatgcggcc cgctggtcgg tgactggtca cagatcgatt tatcccgcct
      961 gccgttctat ctcaatgccg acagtccggt ggcctgtgcg ctgcaccggg ctttaacgct
     1021 tggtacgcag cagttctggt tgcgtctgcc ggggcaggat cgccggacgc ttgacgcaca
     1081 cttttccccg ttgggctttg aggataatga caggctctgg ccaaaaggcg agtcggcgtt
     1141 cagcggctac cagttgctgc tggagtattt caccttccgc gagaagttta tgtttgtggc
     1201 actcaacggg cttgagctgg ttgcatggcc ggaggggata accggctttg agattgacgt
     1261 ggtgctgaac gagaactggc ctcacgacct gccgtttgac agcgataata tccgtctgca
     1321 ctgcgtgccg gtcatcaatc tgttcccgct cgaagccgat ccgttacacc tttcgccact
     1381 ggaaaacgaa tttctgctac gcccgatgcg cattcaggac gggcataccg agatttattc
     1441 cgtcgacaac attatttctt cgcgccatac cggcagtcag gcgtatgtcc ccttctccag
     1501 ctttcgtcac cggggcggga tgctgcgcca cgatgcgccg gaacgctatt accacacgcg
     1561 ggtaaagcgc ggcccttccg gtctgcatga cacctggctg attctgggcg gtgatgcgtt
     1621 tgatgctgac cggatgctgg aggatgaaac gctctcttta agcctgaccg gtactaacgg
     1681 gcatcttccc cgtaaggctt tacagagcac gctgctcgat aggccagttc atgcctcaca
     1741 gaacgtcctg cgggtgcgca acctgtgcgc cccaacgcag ccctgctatc ccccggcgcg
     1801 tgaccgcttc cactggcggg tgctcagcca ccttggttct aactttttat cgatgatgga
     1861 taacgccgaa attctgcgcg gaacgctggc gctgtacgac tggaccgaaa gcgagatgaa
     1921 ccgccgtcgt ctggaggcga ttgtcgacgt tcagcacagc ctgatacagc gttttgaaag
     1981 aggctttctg ctgcgtggcg tggatattca ggtgacgctg gacagtaacg gctttgccgg
     2041 agaaggtgat atcacgctgt ttggtgaact gctgcaccgc tttttcgcac tgtatgccga
     2101 tatccactta ttcactcaac tgaccttaat tctgcaacct acaggaaagt gcctgcaatg
     2161 gacagagcat cacagccagc gcgtaccggg ctgaccgacc ggctggcccc ggatattgcg
     2221 cggatcaatt tttaccgttt ttgccagttt ctggaacaga gccagcagaa cgcgccgctg
     2281 ggcagtaccg acaaccccgc caccgatgcg gtgcgctttc gtccccatcc cggaatgggc
     2341 tttccggtca gcgagttaaa gaacgttgaa cgtgacgcgg gcaatcccaa cgtgccaccc
     2401 acggtgcgca ccacctttct cggcctgtac ggcgtggatt cgccgctgcc aaccgcctat
     2461 ctggactaca ttacccagcg tcatgacgga catgacgcag tgatggcgtt tctcgatatt
     2521 tttaaccatc gctttatcac ccagtattac cgcatctggc gcaaatataa ctacccggcg
     2581 tcgtttgaag caggtgctat ggatgacatt tcccgttgtc tgctgggact catcgggctg
     2641 ggtattcccg gcagcgagaa ccatatcgcc acgccggtat cccgttttct ggccctgctc
     2701 agcgtgatgc gcctgccgac gcgcacggcg gaaggcgtca cggcactggt gggactgctt
     2761 gccccgctga cgaaagcgac ggtggtgcct cacgatccgc agcctgttat tctcccggcc
     2821 cctgcgggtc tgtcaaaaaa ttcacgcatc agcctgaaaa cgcgaacgct gctggggcgt
     2881 accggcaccg acgtcaacag ccagttgctg ctgaaactgt ataccgaaga tgccgcagaa
     2941 gcacgtggct ggctacctgg cgggcagctg cacagcgatt tgctggtgct gctgcgcgtt
     3001 tatctgggct ggcgctgtca ggcccgtctg caactgacgc tgcccgtcag cctgttgcct
     3061 gctgcccggc tgggaaaaca gcgcgtacag attagccgta ccggcatcct tcgcgcctcc
     3121 tttgccgcgc ccgcgaccgg aacagtgacg gtcagtctgg ggcgttatca gggattaata
     3181 cccgctttat ccattcgtaa cagggagagt atgactcatg tttcctattc gttttaaacg
     3241 tccggcgctg ctctgtatgg cgatgctgac ggttgttctg agcggctgcg ggctgattca
     3301 gaaagtggtg gatgaatcga aaagcgtggc ctcagccgtt ttctacaagc aaatcaaaat
     3361 actgcatctc gatttcttct cccgaagtgc cctgaatacg gatgcggaag atacgccgct
     3421 ttccacgatg gtgcatgtct ggcaactgaa aacccgcgaa gattttgaca aggcggatta
     3481 tgacaccctg tttatgcagg aagagaagac gctggagaag gacgtactgg caaaacacac
     3541 cgtctgggta aaaccggaag gcacggcatc cctgaatgtg ccgctggata aagagacgca
     3601 gtttgtcgcc attattgggc agttttatca ccctgatgaa aaaagcgaca gctggcgtct
     3661 ggtgatcaaa agggacgaac tggaggccga caagccgcgc tcgattgaac tgatgagaag
     3721 cgacctgcga ctgctgcctc tcaaggataa ataaccgatg tcgacacctt ccttatatga
     3781 aatgctgacg ttcagtttca gcggcgagtt accactggaa caggtcagcg agcgggatca
     3841 gcttatcctt tccgtgatgg acaatatgca acgcattatc aactgtcggg caggcacact
     3901 ggcgcatttg ccggattacg gtctgccgga tctcagtctg atccatcagg gtatggttgc
     3961 gggtattcat ggcctgatgc gtcagattga agaaacgctg ctgcgttatg aaccacgcct
     4021 gagtcagata caggtggagt tactccccca gccccgtccg gggtatctta attacctgat
     4081 ccacgcgcag cttcccgata ccggctggat acgctttgat ggcgtgtttt ctccggaagg
     4141 acgaattgtt ctgcgtcatc tcaaacagca ggagcgggcg tactgatggc aagtaacgcg
     4201 aattttatca gccagttcgt catgggcggc gatccctgta cttataagga atccggtgaa
     4261 ctacaggctg aaatgagtaa actgactcac ccggcccgac ctgatgtgga ctggcgtcgg
     4321 gtggaaaaac tcagcctcgc gctgttccgg caaaatggcg tggaattaca gacgcaggtc
     4381 tgttacgtac tggcgataac cagacggcag gggctggcag ggatggcaga cggactcggt
     4441 tcactggaca tactgctcca gcgctgggct gacttctggc cggtacaggt acattccaga
     4501 atatcactgc tcagttgggt cacagaaaaa atgcagcagg cactgagaac gctggatatt
     4561 cagtatcagg atctgccgca gatttaccgt tgtgtacagc atctttccgc catcgaaacc
     4621 acgctgcaac agtgtgaact gtggcatatg acgaaactgg atgtgctggc cgggcagttt
     4681 cgtaatacgg cgctacgtct ggagcggctg gcaccacagg gagcggaaac cactatcact
     4741 ccccctgaat taccccgccg ggaaatgaag caaccagaaa agtcagaaga aagtccacag
     4801 ccggtttttg caaccagacc cgctcagcaa aacgataagg atgccagtcc atccgcgcca
     4861 tcccctgaaa tctcctggca gcggacatgg ccgatattta tggccggaat ggttgtgatg
     4921 gcctgtctcg gcggaacagg attatggggc tggtcgcagc ttaatcagcc ggacgcacta
     4981 atccagcgaa tacaactgtc tgtcatgcca ttaccgcagt cgctggagag cggcgagctg
     5041 gcaaagctgg atgtaaagga taaggcgctg ctggctcagg acagaacaat tgcggcaagt
     5101 cagatgcaac tggagcagtt aaacaaattg cctgcccgct ggccactgga gcagggatat
     5161 cgccagctac gccagcttga tgccctgtgg ccggataatc ctcaggtcag agcgctgaac
     5221 gcgcagtggc gtaaacagcg ggagctgagc gccctgtcca cagaggcatt gaatggctac
     5281 gcacaggcgc agagccagct acagcgcctg tcggcgcagc tggatgcact ggatgagcgt
     5341 aaggggagat atctgaccgg ttcggaacta aaaacggcgg tgtacggcat ccggcagtcg
     5401 ttaaaggagc cgccgctgga agaactgctt cggcaactgg aagagcaaaa acagaccgga
     5461 gaggtttcgc cgacgctgtt gacgcaaatt gatacccggt taaatcagtt gctgaatcgc
     5521 tatgtaattt tactggatac gaaggtggaa caaagtcagt aaacgtcggg ggatttatgc
     5581 accaataagg gatatgtgac cttgccattt tttaaaagga gtctttgtgt acaaaaaaga
     5641 gaggaacagg ccattcgaaa cgcgtgaaga ggttctggca tatcataagc gggaaaaaat
     5701 accctgtctg gagtgtggca agttgctcat ttttttaccg cgccatatct ggtttgtgca
     5761 tggaatacgc gctgacgaat atcgtgtaaa atggaacata ccgaagcaca tcgcactggc
     5821 agggatttca tatctggaga aacaaagtga atatatgaag gcgagaatag caaagggaaa
     5881 gctggatccc gcagaacaaa tagccatgat gagaataaag ggaaggcaac taaagtcaga
     5941 tcctgactgg cgtaatcgct ccggttctat attgaagaaa caatatgccc gacgagatat
     6001 ccttaaaaac agggtatggg aaagatctcc cagcataaaa agtgtctctg tggagttgaa
     6061 aacagaagct ataaggcgaa tgaaaaacag gaaggtcgtc ggggaaaaag tccaggatat
     6121 tgccgatgac ctgaacgtat ctatcagcag attgtatcac tggctaaaga tgtaggcgct
     6181 catgtgctct caatgccttc aataaagccg ctatgcgcga accggcacat ctggtagact
     6241 gttctcagtg agtcaagcaa actgcctact tttctaactt ttaatcatga ataaccagcg
     6301 ccttgagtat agctggttcg aatatactcg ttgattcaca tcagataatg agtgtataat
     6361 tcggtagata gcttgggttc gggaacagtg aatgccttaa gtttcaaaag gttagattaa
     6421 cctcttgact gtggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
     6481 ctgctctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
     6541 tcgccccatc ggcaaaatac gctttaattc ccgcaagaat agactccgca acttcctgct
     6601 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
     6661 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
     6721 ttttatgcag cttgttgatt ttaccgagct tattcagtac cgctttacca aacttcaggc
     6781 tgtcggcaat ggtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
     6841 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
     6901 ccgcagtact ggttgcacct ttggttgaga gcgcaaacac agaggaaccg ctcggctgac
     6961 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
     7021 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
     7081 tattgccctc tttctcgatc agagagcgca aacggcgagc tatttgcaat accacgtctt
     7141 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa
     7201 gcataatgac aatcggacga tcgcgccctg ctttacctgg ttgtggacca ctttgcgctg
     7261 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
     7321 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
     7381 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatcc
     7441 gtacggtttg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
     7501 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
     7561 ccacgcgttc cgggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
     7621 ccgtcacacg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctgaccg
     7681 cagccagact gacctgactt acgctcaata gccacatggc acccgcgcct tgcagtaaac
     7741 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
     7801 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
     7861 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
     7921 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata cactaatttc
     7981 gaataatcat gcaaagaggt gtgccgtggt aaaggaacgt aaaaccgagt tggtcgaggg
     8041 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
     8101 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttatt
     8161 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
     8221 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
     8281 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
     8341 gtcgatgagt ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg tcagtggcaa
     8401 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
     8461 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gacagcggtg caatagtgct
     8521 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
     8581 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
     8641 gggcgttact aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc
     8701 acgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
     8761 tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga
     8821 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
     8881 ggaaagcgct gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
     8941 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
     9001 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
     9061 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
     9121 ccgcagctca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
     9181 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
     9241 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
     9301 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaattg tcggatgcga
     9361 catgcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
     9421 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
     9481 tcggcataca gcgacagacg gcgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
     9541 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
     9601 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
     9661 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
     9721 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
     9781 accggtcgac cttcgtacca acgagagtgc ggatgacgat gaattttgca cttctgttgc
     9841 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
     9901 cacaaaagtt ggtactcatt gaacgcctga atgattaaat ccggctcagc acgcgcttgc
     9961 agcagatcca gataacgtcc ataacccgca agagcttcct caagcatcgc aatataatct
    10021 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    10081 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
    10141 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    10201 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcccggc acgctcggcg
    10261 gtaaagccgg cattggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    10321 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc
    10381 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    10441 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc acccagcaat
    10501 cggtgcaaag tactggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    10561 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    10621 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagtaacttc
    10681 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    10741 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    10801 tccagggttt gcgccagtag agcttcatca acctcaatgg catgattcac ctcattgaag
    10861 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    10921 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    10981 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
    11041 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    11101 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
    11161 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    11221 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg tcaatcaacc
    11281 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acaccacgca
    11341 ggaacagata aataacgccg ccaaagtggc gctcatagtc gtaatcagca atgcgatggc
    11401 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    11461 gcatcgccgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    11521 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    11581 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcggatcaa
    11641 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcactaatc ggcaggtaaa
    11701 actccatctc cacctgttta tcgcggtcgg aaagctgatt caggctaaca cccgtttcat
    11761 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa cactggttcc cactgcgatt
    11821 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    11881 cgaggtcttc aaacaaactg tgcaagaatg tccctggtga cgcaccgcgc gggaactgat
    11941 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    12001 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caaaccggag tagctggtga
    12061 cacgccagtt atcgccgggc aatcgttgta gcgtcctcgc attcagttct gcagttaaag
    12121 catcattaac ctgccagggc tgtttatcac ccgtttgtgc cgtttgccag gcaatatcat
    12181 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    12241 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    12301 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgtgtc agcgccacgt
    12361 aaagcaaacg cagatcttcc gccagacgtt cggcctccgc gaggtcgacg ctttctggcg
    12421 cagcattaag atccagaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    12481 ggacgcggaa attggtgata aacggcagcc agaccaatgg atattccagc cctttcgatt
    12541 tgtggatcgt gacaatctgc accagatgtt tatcactttc gagacgcatt tgttggctgg
    12601 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    12661 tttccagctg cgttccggct tcttgtagca gttcgctgat atgcaagata tcggtaagtc
    12721 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    12781 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    12841 cgaccaccgc gtcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca
    12901 gccccatcat tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca
    12961 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    13021 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    13081 cctggcggct gcgcaccagc acactgatgt ccgaagcacg caccggacgc gcgtcgtcgc
    13141 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    13201 cacatacctg cgccatggta ctttgataat cgccaacgcc gcagctttcg ccttccatca
    13261 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    13321 tcccgactga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
    13381 ggctgaaaag cttattcacg ctgttcacca ttcctggtgc ggaacgccag ttggtgtcta
    13441 aagtgtagtg ggcgtgaact tcgctacgcg ccttcatata agtgaagata tccgcacccc
    13501 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    13561 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    13621 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcaccg ctttcgctac
    13681 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    13741 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcacc agatcgcgga
    13801 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
    13861 cccccccggc tttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    13921 gcaactgata actgttggtc tcttcttctg cccaggcgct gatcttgtcg atccatttag
    13981 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    14041 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtagc aatacgcgcc acaatttgcg
    14101 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
    14161 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    14221 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    14281 cctggtaacg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    14341 caaaggcatt caggttgagc atgcgctggc aaaaaccgtg aatagtaaag actgccgctt
    14401 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    14461 cttccagcag acgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    14521 actcatggat attgctacgg atacgaccac gcaattctgc cgtggcagcc tcggtaaagg
    14581 tgaccaccag cagttcttca acggtcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    14641 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    14701 cttcaatcag gcgctcaccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    14761 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    14821 actttccagc cttcagggtg tacatattcg gctttcccat tctggctgcc ggaaatctgc
    14881 gacaggatag ccatgccttg cggctcgact accgcctgat ggaagaaatc ggccagtttt
    14941 tgcggcgtca gcagtttaat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    15001 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    15061 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    15121 aattttgcct ctgcagttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    15181 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    15241 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    15301 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    15361 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    15421 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    15481 tgtttttgca catcgcgtgc cagcgttgtt gcctgggctt cggtcatgtt gccaataacc
    15541 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    15601 gtaatggagg gcaaaatttt gcgccgttca tctcgcgaga agtacggcac ttgcgagagc
    15661 atctgcgcgg gcataatcgc ctgctcgaac gctttgccct tttctgcgga atccatcatc
    15721 tggttatacc aggacttcgc ctgctcaagc tgatcttccg tggcggtata gctaaagtag
    15781 ccttccagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    15841 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    15901 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    15961 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcggcttt tggctcgctg
    16021 gcaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    16081 ggatggtcat atttcttgtc tgacttaatc agcgagaaat cgtcaggaat ataagggtta
    16141 agctctggca aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt
    16201 gcgctgattt tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc
    16261 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc
    16321 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca
    16381 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgattgac
    16441 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcgatc
    16501 cctttttcac gtaacaaatt gaggtagcta aaaatagccg ccacaacctg atcgcgattc
    16561 gccagacctt tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    16621 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcggaa
    16681 agtgtacctg ggctgcgatt accaatcaga taggtaatca attcatcggt tttgctacgg
    16741 aatttcgccg agttgttatc aatacgaaac tctacgcgca acactttacg cggcagcgcc
    16801 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    16861 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    16921 tccggcagcg gtttattgct gtaaataacc gccttcatca gattggcgga atagtacttc
    16981 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg
    17041 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    17101 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta
    17161 cgctcacgtt cggcgtattt cttgtcgagc aagggttctg caatagcatc agccaggcgg
    17221 tctaccgcac caggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    17281 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    17341 tgcgggtact ttttcgcccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    17401 tacgcctcgg gatcttccag cgacccaacg ggcaccacca gcgccgagag cgatttaact
    17461 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    17521 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    17581 gcctgactta agggtgccca aagggcaact aacaacaata atgctttgaa ccaggtgctg
    17641 cggggcattc acggacctca taatcaactt aattttctgt ccagattcaa cacgttaacg
    17701 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    17761 tcagtccgtg actggtgcgc atcataaagc aagcggatag attgcgcaat ttttatacag
    17821 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    17881 ggcctccatt gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc
    17941 ttcgccacgc accatcatgt tgccttcgta agcctgcagg aatttcgtac gggctttttg
    18001 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    18061 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac atcccttcac gatacccctc
    18121 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    18181 gccgtctttg cgtagaaaaa ggcgactttc accattacca ccgctggcac agtagacaag
    18241 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    18301 caagccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    18361 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    18421 catctcatgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    18481 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    18541 taactgctga ttgatttgat aacgactaag cccttcaaga ataaatggct cggtgtcggg
    18601 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    18661 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    18721 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    18781 acgagcatag ctttgtcgtt cgcctggctg gtagttttgt ggatcaaacg gcatccgggt
    18841 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    18901 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    18961 cgggaaacgt tcactgttat cctgaatgga gcgcccgata tagctgatat aaagtttttg
    19021 ttgcgcggaa attaacgctt ccaggaacag atagcggtca tcatcgcgac ggctacggtc
    19081 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    19141 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    19201 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    19261 acgctgcgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    19321 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    19381 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    19441 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    19501 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    19561 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    19621 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    19681 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    19741 gtcaaaccgc gccgccagca ccggcacatc cagtaatgcc agcacgtctt ccgacacaaa
    19801 gcggctgtct ggcaacgata acaggctgat aaacgcttcc agtaccggat gcgactgccg
    19861 cgcccgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    19921 agcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    19981 agtaagtgtc gggtcttctt ccagcatcgc cagcaggcga tcgtgcaaaa cttcaacttc
    20041 acgctgcggg ctatggcaaa cgtggaaggt gatactgcta tccagtggat caagcgggcg
    20101 tttgttatcg ctacgggaaa actcttcgat gttcacacca gcaacggcac ggttttccag
    20161 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtgacat cgacaaaggc
    20221 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    20281 accccatgaa gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    20341 cccggcattt tcgctatcac gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    20401 ctgacgggtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    20461 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct gtagcgcctg
    20521 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtagccccgg
    20581 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tggcgcggtg
    20641 ccagcgcggt tgcccgagtt gatgggtata ttccaccaac gccttccaca acggcgcttg
    20701 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    20761 gtccggacga tagaccagat actggtcaaa caggtccgcc gcttttgaag aaagctggaa
    20821 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    20881 ctccaacaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    20941 gctctctttg gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    21001 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    21061 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    21121 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    21181 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    21241 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    21301 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    21361 ctgccaacca tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa
    21421 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat
    21481 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa cgccctctct
    21541 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatgtttcc atcaatgact
    21601 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    21661 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    21721 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    21781 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    21841 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    21901 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    21961 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    22021 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    22081 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    22141 tcagacgaaa tccaatctgg tcagactctt ttaccggttc gcgatcccag ataccgttac
    22201 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    22261 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    22321 gcagccagat ttcatcttcc agcgccagct tacgggtgct cgttaaactt tcacgctgta
    22381 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    22441 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    22501 acaaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    22561 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    22621 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    22681 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    22741 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    22801 ccgcgcctgg ctggcggttt gccatagccg ttgcgactgc tgccagtatt gccagccata
    22861 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    22921 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    22981 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    23041 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaaccgacg cacactggcg
    23101 tcggctctgg caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg
    23161 gatcgtagcc ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac
    23221 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    23281 ccatatgatt gctgtacagg tgcgtgtcgc ctcccgtcca gacaaaatcg cccacctcca
    23341 gatcacactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttaaacggca
    23401 ggccgaggaa aacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    23461 catagaactg gaagaacgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    23521 tccacgctga aacaataatg cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg
    23581 tagtgatctg gtcaatatga cgaccatccg gcgttggcca ggcgcgccac tgtttaccat
    23641 acactggccc gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    23701 cgtgtagata agcaatgtta gtgtcgccct gcagaaacca cagcagttca tggatgatgg
    23761 aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    23821 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    23881 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    23941 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    24001 tggaaagaat ttgccccatg ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg
    24061 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta gccaatcagg aacaagccag
    24121 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca
    24181 caccttccag cagcagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt
    24241 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    24301 ctgggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    24361 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag
    24421 aaacctggaa gaaggaacgt ttagtacggc gggcaaagat aatcatcacc acgataacgc
    24481 caatcaggcc gccgtggaaa gacatgccgc cgtcccagac acggaacaga tacagcggat
    24541 cggccataaa ctgcgggaaa ttgtagaaca gaacataacc aatacgtccc ccgaggaaga
    24601 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccgctgcccg
    24661 gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    24721 ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    24781 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    24841 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    24901 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    24961 ctccgcgaat cagcccgccc atgccgcgac gctccataaa ggctgcaacc tgatggcgaa
    25021 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcggcataat
    25081 caatgcgccg cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc
    25141 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    25201 aacggagatc gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca
    25261 ttgcaggatg aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca
    25321 gaatgtattg agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    25381 gcggcagcat aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa
    25441 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    25501 catcaacttc atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    25561 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    25621 tgcgaatccc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    25681 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca
    25741 gcatcccctg atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga
    25801 atgggatttc agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat
    25861 gttccgggct taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg
    25921 cgggtaaatt gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc
    25981 gctgatattc ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat
    26041 caacgatcag cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag
    26101 ggatccccag cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga
    26161 caacaccgac taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg
    26221 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    26281 acagcaatcg ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    26341 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    26401 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt
    26461 gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc accggcggca aagcgtttgc
    26521 tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca
    26581 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    26641 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc
    26701 gatattgccc aaacagggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg
    26761 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    26821 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    26881 aacgttcttc ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    26941 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    27001 ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc
    27061 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    27121 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    27181 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    27241 cgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    27301 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac
    27361 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    27421 cgccgctcac cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    27481 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    27541 ggattcgaac gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct
    27601 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    27661 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    27721 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    27781 gtttcatact ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    27841 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    27901 tattcacctt ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg
    27961 gag
//
LOCUS       DAZFYC010000062        27833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFYC010000062 DAZFYC010000000
VERSION     DAZFYC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.062"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..737
                     /locus_tag="ACHKW5_002857"
     CDS             54..737
                     /locus_tag="ACHKW5_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512216.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            753..1163
                     /locus_tag="ACHKW5_002858"
     CDS             753..1163
                     /locus_tag="ACHKW5_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512217.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            1384..2205
                     /locus_tag="ACHKW5_002859"
     CDS             1384..2205
                     /locus_tag="ACHKW5_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7512218.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGKVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2287..2766
                     /locus_tag="ACHKW5_002860"
     CDS             2287..2766
                     /locus_tag="ACHKW5_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7512219.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPLWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2782..3258
                     /gene="yeeS"
                     /locus_tag="ACHKW5_002861"
     CDS             2782..3258
                     /gene="yeeS"
                     /locus_tag="ACHKW5_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7512220.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3321..3542
                     /gene="yeeT"
                     /locus_tag="ACHKW5_002862"
     CDS             3321..3542
                     /gene="yeeT"
                     /locus_tag="ACHKW5_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7512221.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3616..3984
                     /gene="cbeA"
                     /locus_tag="ACHKW5_002863"
     CDS             3616..3984
                     /gene="cbeA"
                     /locus_tag="ACHKW5_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO7512222.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDAAAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            4073..4446
                     /gene="cbtA"
                     /locus_tag="ACHKW5_002864"
                     /pseudo
     CDS             4073..4446
                     /gene="cbtA"
                     /locus_tag="ACHKW5_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4443..4637
                     /gene="yeeW"
                     /locus_tag="ACHKW5_002865"
     CDS             4443..4637
                     /gene="yeeW"
                     /locus_tag="ACHKW5_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7512223.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4683..4763
                     /locus_tag="ACHKW5_002866"
                     /pseudo
     CDS             4683..4763
                     /locus_tag="ACHKW5_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5052..5198)
                     /locus_tag="ACHKW5_002867"
                     /pseudo
     CDS             complement(5052..5198)
                     /locus_tag="ACHKW5_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5252..5434
                     /locus_tag="ACHKW5_002868"
     CDS             5252..5434
                     /locus_tag="ACHKW5_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO7512224.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(5535..5864)
                     /gene="tmaR"
                     /locus_tag="ACHKW5_002869"
     CDS             complement(5535..5864)
                     /gene="tmaR"
                     /locus_tag="ACHKW5_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO7512225.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6036..7094)
                     /gene="yeeA"
                     /locus_tag="ACHKW5_002870"
     CDS             complement(6036..7094)
                     /gene="yeeA"
                     /locus_tag="ACHKW5_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7512226.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ACHKW5_002871"
     CDS             complement(7292..7765)
                     /gene="sbmC"
                     /locus_tag="ACHKW5_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO7512227.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCGTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7884..9050)
                     /gene="dacD"
                     /locus_tag="ACHKW5_002872"
     CDS             complement(7884..9050)
                     /gene="dacD"
                     /locus_tag="ACHKW5_002872"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO7512228.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            9259..10686
                     /gene="sbcB"
                     /locus_tag="ACHKW5_002873"
     CDS             9259..10686
                     /gene="sbcB"
                     /locus_tag="ACHKW5_002873"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO7512229.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(10729..10956)
                     /gene="tsuB"
                     /locus_tag="ACHKW5_002874"
     CDS             complement(10729..10956)
                     /gene="tsuB"
                     /locus_tag="ACHKW5_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO7512230.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10970..12028)
                     /gene="tsuA"
                     /locus_tag="ACHKW5_002875"
     CDS             complement(10970..12028)
                     /gene="tsuA"
                     /locus_tag="ACHKW5_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO7512231.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(12207..13565)
                     /gene="plaP"
                     /locus_tag="ACHKW5_002876"
     CDS             complement(12207..13565)
                     /gene="plaP"
                     /locus_tag="ACHKW5_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO7512232.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(13555..13617)
                     /gene="yoeI"
                     /locus_tag="ACHKW5_002877"
     CDS             complement(13555..13617)
                     /gene="yoeI"
                     /locus_tag="ACHKW5_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO7512233.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(13832..14761)
                     /gene="yeeY"
                     /locus_tag="ACHKW5_002878"
     CDS             complement(13832..14761)
                     /gene="yeeY"
                     /locus_tag="ACHKW5_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7512234.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(14807..15631)
                     /gene="yeeZ"
                     /locus_tag="ACHKW5_002879"
     CDS             complement(14807..15631)
                     /gene="yeeZ"
                     /locus_tag="ACHKW5_002879"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7512235.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(15714..15968)
                     /gene="yoeB"
                     /locus_tag="ACHKW5_002880"
     CDS             complement(15714..15968)
                     /gene="yoeB"
                     /locus_tag="ACHKW5_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO7512236.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(15965..16216)
                     /gene="yefM"
                     /locus_tag="ACHKW5_002881"
     CDS             complement(15965..16216)
                     /gene="yefM"
                     /locus_tag="ACHKW5_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO7512237.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            16499..16549
                     /gene="hisL"
                     /locus_tag="ACHKW5_002882"
     CDS             16499..16549
                     /gene="hisL"
                     /locus_tag="ACHKW5_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO7512238.1"
                     /translation="MIRVQFKHHHHHHHPD"
     misc_feature    16525..16648
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            16695..17594
                     /gene="hisG"
                     /locus_tag="ACHKW5_002883"
     CDS             16695..17594
                     /gene="hisG"
                     /locus_tag="ACHKW5_002883"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO7512239.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            17600..18904
                     /gene="hisD"
                     /locus_tag="ACHKW5_002884"
     CDS             17600..18904
                     /gene="hisD"
                     /locus_tag="ACHKW5_002884"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO7512240.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNVETFHTAQ
                     KLPPIDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNIGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDTD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            18901..19971
                     /gene="hisC"
                     /locus_tag="ACHKW5_002885"
     CDS             18901..19971
                     /gene="hisC"
                     /locus_tag="ACHKW5_002885"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO7512241.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDS
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            19971..21038
                     /gene="hisB"
                     /locus_tag="ACHKW5_002886"
     CDS             19971..21038
                     /gene="hisB"
                     /locus_tag="ACHKW5_002886"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO7512242.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILSWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGMTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            21038..21628
                     /gene="hisH"
                     /locus_tag="ACHKW5_002887"
     CDS             21038..21628
                     /gene="hisH"
                     /locus_tag="ACHKW5_002887"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO7512243.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            21628..22365
                     /gene="hisA"
                     /locus_tag="ACHKW5_002888"
     CDS             21628..22365
                     /gene="hisA"
                     /locus_tag="ACHKW5_002888"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO7512244.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSQDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            22347..23123
                     /gene="hisF"
                     /locus_tag="ACHKW5_002889"
     CDS             22347..23123
                     /gene="hisF"
                     /locus_tag="ACHKW5_002889"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO7512245.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIALNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            23117..23728
                     /gene="hisIE"
                     /locus_tag="ACHKW5_002890"
     CDS             23117..23728
                     /gene="hisIE"
                     /locus_tag="ACHKW5_002890"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO7512246.1"
                     /translation="MLTEQQRRKLDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(23825..24805)
                     /gene="wzzB"
                     /locus_tag="ACHKW5_002891"
     CDS             complement(23825..24805)
                     /gene="wzzB"
                     /locus_tag="ACHKW5_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO7512247.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDAGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGVQKQLAKYIQQVDDQVN
                     VELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(24952..26118)
                     /gene="ugd"
                     /locus_tag="ACHKW5_002892"
     CDS             complement(24952..26118)
                     /gene="ugd"
                     /locus_tag="ACHKW5_002892"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO7512248.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQHFLQSDKIHFNATLDKNEAYRGANYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(26367..27773)
                     /gene="gndA"
                     /locus_tag="ACHKW5_002893"
     CDS             complement(26367..27773)
                     /gene="gndA"
                     /locus_tag="ACHKW5_002893"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO7512249.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         7173 a         7162 c         6842 g         6656 t
ORIGIN      
        1 ctgagagaaa gttttacccc tcacctgtca attcactcac tggaaattta attatgaaat
       61 tacctgtaaa gttattaatg agccttatat ctctggtcag cgttattgca cgtgccgggg
      121 aatataaaaa ttacagccgg gatgaaataa aatactggcg atatacatca tacaaggggg
      181 ggaaacttcc ggaaggtttc actgatgaga aattttccag cgccatttac aacggaagaa
      241 tatttacaat gaaacgttta cataccctta tgttatttct ggcggttctg ttcacgggct
      301 ttaactttaa cgcagaagcg gctaccgtaa aacaagctct tagttgtaac ccggaagcct
      361 gggctgaaca acctggagcg tgtccatcaa cgtacgagtt gtacgaaggt gacgctacct
      421 acaaagccgc tatcgacaaa gcattaaaac cggtcggact gagcggcatg ttcggtaaag
      481 gcggctatat ggatggccct ggtggaggta tcacgccagt aaacattaac ggtacagtct
      541 ggttccaggg cgacggttgc aaagccaata cctgcggctg ggactttatc gtaacactct
      601 ataacccaaa aacccatgaa gttgttggct accgttactt tggtttagat gacccggcct
      661 acctggtttg gttcggtgaa attggcgtgc atgaattcgc gtatctggtg aaaaactacg
      721 tagctgcggt taactaattt aaggagtcta tcatgaaaac ttttatcaaa actttactcg
      781 ttgctgtaac tattctgttc tctgtcttcg ctactgcgaa acaagtaaaa ctgccaaata
      841 acatcaaata cgttaatact acagaagcgt tttcctgtac cgaaattgac ggtatgaatt
      901 gccagacaaa gaatcagttc aactataaag ataacagcta tgttttcgtg cttgaacgtg
      961 gtggtgcctg gtgctacgac tacactgtct cggtagttaa cctgaaaacc gggaaagcac
     1021 aaatgattga atacggggac aatcaactgt gctcaggtag caacaaaccg ttcttcgaaa
     1081 tcaaaaatgg cgtaccgacg gtaggagtca tcgacacatc cggaaaacct gtcgttgtag
     1141 cccaggacaa acttaaaatc taaggagtaa accatgcgtt tctctgcaca ttattaatat
     1201 caaaggtaaa tacagcccca gccattgtta ccggaaagcc gcttaatgcg tggctcgcca
     1261 gtgaaaggcc tctcagacaa taaaccttat tcatttcacc cgtcaggccg cgtcttctcc
     1321 gggagacgcg gcttttttca tttattgcac cagtaaatct taaccaccga taaggagcaa
     1381 agtatgcgat tagcaagtcg ttttggttat gctgcaaacc agatacgccg tgaccgtccg
     1441 ctgacacatg aagaactgat acgccatgta cccagtattt ttggggaaga ccggcacacc
     1501 tcccgcagtg aacggtatgc gtacattccc accattaccg tcctggaaaa tctgcagcgg
     1561 gaaggctttc agccgttctt cgcctgccag acccgtgtgc gcgacccggg ccgccgggga
     1621 tacacaaaac acatgctgcg tctgcggcgg gccggagaga taaacggaga acatgtccct
     1681 gaaattattc tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac
     1741 ttcaggttcg tctgccagaa cgggtgcgtc tgtggccagt ctctggggaa agtgcgtgtt
     1801 ccacaccggg gaaatgtagt ggagaaagtt atcgaagggg cttacgaggt ggtgggcgtg
     1861 tttgaccgga tagaggagaa gcgtgatgcc atgcagtcgc tggtcctgcc gccaccggca
     1921 cgccaggcgc tggcacaggc ggcactgact taccgttatg gtgacgaaca tcagcccgtc
     1981 accaccgccg acattctgac gccgcgacgc cgggaggatt acggtaagga cctgtggagc
     2041 gcatatcaga ccatccagga gaatatgctg aaaggcggga tttccggtcg cagtgcaaaa
     2101 ggaaaacgta tccatacccg ggccattcac agcattgata ccgacattaa gctcaatcgc
     2161 gcattgtggg tgatggcaga aacgatgctg gagagcctgc gctgatgccg tttccctgag
     2221 tgagggaatc attcagcttt cgttcctgaa aaacacacat ttatttgtta tttaaggagt
     2281 tatctcatga aaaccgtttc tcagaatacc cccacaattt attcagctac aacaccagag
     2341 aataatccgc ctcagttggt tgccagcctc gtccctgatg aacagcgcat cagcttctgg
     2401 ccgcagcatt ttggcctcat tccactgtgg gtgaccctgg agccccgtgt cttcggctgg
     2461 atggaccgtc tgtgcgaaga ctactgcggt ggtatctgga atctgtacac cctgaacaac
     2521 ggcggggcat ttatggcacc cgaaccggat gacgatgatg acgaaacatg ggtactgttc
     2581 aatgccatga acggtaaccg cgcagaaatg agcccggaag ccgccggtat tgccgcctgt
     2641 ctgatgacgt acagtcatca tgcctgtcgt acggagaatt atgccatgac ggtccattat
     2701 taccggttgc gggattacgc cctgcagcat ccggaatgca gcgccattat gcgcatcatc
     2761 gactgaatgg agagaagcac aatgcaacaa ctttcctttc tgcccggaga gatgacgccc
     2821 ggcgagcgca gcctcattca acgggccctg aaaaccctgg accgccatct tcatgaaccc
     2881 ggcgtggcct tcacctccac ccgtgcagca cgggaatggc tgattctgaa catggcggga
     2941 ctggagcgtg aagaattccg ggtgctgtat ctgaataacc agaatcaact gattgccggt
     3001 gaaaccctct ttaccggcac catcaaccgc acggaagtcc atccccggga agtgattaaa
     3061 cgcgccctgt accacaatgc cgctgccgtg gtgctggcac acaatcaccc ctccggtgaa
     3121 gtcacaccca gtaaggcaga ccgccttatc acggaacgtc tggtacaggc actgggcctg
     3181 gtggatatcc gggtgccgga ccatctgata gtcggtggca accaggtttt ctcctttgcc
     3241 gaacatggtc tgctttaacc cttcacaacc acatcacacc tgttttcact tttatcttct
     3301 gtcttcagag gtatcccatt atgaaaatta tcacccgtgg tgaagccatg cgtattcacc
     3361 aacaacatcc gacatcccgt ctttttccgt tctgtaccgg taaataccgc tggcacggca
     3421 gtgctgaagc gtataccggt cgtgaagtgc aggatattcc cggtgtgctg gccgtgtttg
     3481 ctgaacgccg taaggacagt tttggtccgt atgtccggct gatgagcgtc accctgaact
     3541 gagtgggaat tctgatgagc agaattatcg ccactaccgg accattctta gccgattttc
     3601 tgtaaggatt ttatcgtgtc agacacactc cccgggacaa cacttcccga cgacaatcac
     3661 gaccgcccct ggtgggggct gccctgcacc gtgacgccct gtttcggggc acgtctggtg
     3721 caggagggta accggttgca ttaccttgcc gaccgcgccg gtatcagagg cctgttcagc
     3781 gatgcagctg cgtaccacct ggaccaggcc tttccgctgc tgatgaaaca actggaactc
     3841 atgctcacca gcggtgaact gaatccccgc catcagcata ccgtcacgct gtatgcaaaa
     3901 gggctgacct gcaaagccga taccctcagc agttgtggtt acgtttatct ggctgtttat
     3961 ccgacgcccg aaatgaaaaa ttaactctcc agaatagcct tctgctacgg cctggtgttt
     4021 tcaccacgcc acttttccat ttttatatct gcatatcagg aaaatcttca gtatgaaaac
     4081 attacctgta ttacccgggc aggcgaccag ttctcacccg tctcctgttg aaatctggca
     4141 gatactgctg tcccgactgc tggaccagca ctatggcctc acactgaatg acacaccttt
     4201 tgccgatgaa cgtgtgattg agcagcatat tgaggcaggc atttcactgt gtgatgcggt
     4261 gaactttctc gtggaaaaat acgcgctggt gctaccgacc agccgggatt cagcgcctgt
     4321 acccgctctc agttaataaa cagcatcgat atcctccggg ctcgcagggc gaccggcctg
     4381 atgacccgcg acaattacag aacggtaaat aacattaccc tgggtaagta tccggaggcg
     4441 aaatgatgac actggaagcc gacagcgtta acgtacaggc gctggatatg gggcacattg
     4501 tcgttgacat tgatggtgtt aatatcactg aactgattaa taaggccgct gaaaaaggtt
     4561 attcactccg cgtggtggat gaccgtgact ctaccgaaac accggcaact tatgccagcc
     4621 ctcaccagtt gctgtaagac aatgcagtga tgcagggtat agtgccatta accacagaaa
     4681 cagcactgtt gtcctgtatt aagcaggcaa gagaagatgt ccttaccctg cgccatctgc
     4741 agcttctgca ccagaaccgg tgaatctcac tcaacaggct tcatttcccc tgacgccagc
     4801 ctgaatacag ctggcgtttt catttataaa cagaaaggaa aaccgataat aatggaatgg
     4861 acgccccgac catgaagcaa cggtaggata ttactgataa accttccagt ggaggtgcgt
     4921 tatggccgtt ttaacgatta gcattgacct gacaaaaaat gaattccaaa tccacggtct
     4981 gggtaggaac agaaaaatct aagctcagaa aacggattaa agtaagcgtc aacggagcac
     5041 cgtattgacg cttatttatt ggtgagaact acgttccatg gcaggagttc gtcaacacgg
     5101 ttggagggcc attccggcag tatgctcaga atatggcgca gatacgcttc cggatcgata
     5161 ccgttcagac ggcaggtgcc tgcttttccg gcaacaagca ggaatctcag cgctgaagca
     5221 ggaattagag gtaaaaacgc cctaccgggc catgaatcac ccggtcattg gagtagtaac
     5281 caaagcagat ttagccagca tggaacaaat atctttggtg aaaagttggt tacgggaagc
     5341 tggagcgcac aacgtgttag taaccagtgc agttaataac aatggagtta cagaactttt
     5401 tgccttgctg catacagaag aaggctgttg ttaatttcga ttggttctca gatgagaatg
     5461 atgacggtga tacacagata agaatcaatg agcaatgagg gttgccgggc aaccctcatt
     5521 gaataaaacg ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
     5581 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
     5641 cgatcttcat agtcaccttt catgctggcg atgatgccct ggattgcttc aacgctcatc
     5701 cctggcttga tgtaatcgct caggttgtcc agcaacagga cgcgcttctg gttgtcacgg
     5761 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagacga
     5821 acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat acccctgata
     5881 atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat tactcatagc
     5941 tgccgcagat gacaatgctt ttatcccttt tcactatcat accctttatc cttgctgaat
     6001 cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc
     6061 agattcgata gcaactcaag ctgatgtgcc gtttccatgt tcagccacac ataaccatag
     6121 attggtgttt ccacaacctt caggtcatgg tggttattca gcaactgacg cagctcttcc
     6181 acagcatcgt tcaatttttc cgtattggca aaaaccggct gcggattacc ttcgtacagc
     6241 gcatgaacaa ggctcagcag tatttgctgc atcatatgct gggtatcacg aagtttttgc
     6301 gcgttcaata acacgaaatg gctggggcgc gtggcccagt acgcattgat ttgcaactcc
     6361 agcatacaaa ccagattgcg gttaatggtc tggatacctt catatatcga ttttggaata
     6421 cgggtttctt tgctggcggg cgcaatcagc ccacgcattt tcacggcatc ggtcaagagt
     6481 ttttgtagat ggctttccag acgtgggcgt tcgagtaagt tcggtgagaa tgcagattga
     6541 tagacccgat tatactcggt cagacttttc gccagttgaa tgcgccagtg gatgaacgcc
     6601 cgttgtggcc agataccggt aaacaacatt gccagtaaag agccgaggat cacatcgccg
     6661 cttcgccata acgccgtatc aatttcacct gtcggggaac caacaacaat tgccagcgtc
     6721 accccaatca ataatccttg atacggtttc ttgcccagcg ccagccaacc gcaaagaaac
     6781 atcgccgccg cgcaccagac taacatcagc ggtaacgaaa ttaactccag ttgcagagcg
     6841 ataagaccca aaatcgaacc caacaccgta ccgccaatac gttcgaaggc gcgagggaca
     6901 acgttacccc agaacgagat tggccccata atcaccacca tggtgaccag cggccaggtg
     6961 ctttccggga tagtaaacag gcggataatg agaaaagtga gcaggaatgc cagcgcgacc
     7021 cgagtaccat gcacaatgcg gtaatggcgg tatacccgga tttcaaacgg gcttaatgac
     7081 ttatcggcac gcacgcagaa actccgtaat gaagattaaa agcgctcata actcatctgt
     7141 cggccagaag gcgcgcctct gaattatccg acggacatac cgggaacagt gtaatttccc
     7201 ggcaggagcg aaaaatttga aaggcgcatc ttactctttt cgctttcaaa aaaagagtgg
     7261 tcatcgcgtt aacacaccgc cctgagatga attagtgatg ttttggctgc accgcaacat
     7321 acatttcgat atcccagtac ccatcttccg cgccattgtt caagtaaacc tcgaagcagg
     7381 gctttggtaa catttcataa gcactgtcct gcaagaggct attaaagaac tgataccagg
     7441 gtttagcaaa atcatcaccg actacacgag ctaccgccac cgcatactga ccacctgaaa
     7501 tttctgtcag aatgacgccc tcactgtttt cgggaagcgt aaagtaaccc ggcaccgtca
     7561 cgacggtgcc gcagcgtaat ttttcggcgg gtgtttcatc tggattgtcg taatagatag
     7621 caacccactc cttcggcaca atatttttac tatctaccca catcatcaac tgctcaaaac
     7681 ctttctttac cgtctgttcc caggggccaa cgagatggaa acctgcaacg gtacgtttat
     7741 cttcctgctt aatctcgtag ttcatgacgc ctccattaat actgttttta tatacagtat
     7801 agttgcaata ttaaaaccac aaggaatgag tgttgattat gcgagcagac tcgcactcct
     7861 gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact caggcgagaa
     7921 aacatgctgc cttccccgac agattccagg gtaaccagcg gccagtgcgc cacctgttta
     7981 tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg cgcggtgagt
     8041 tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt cggtagcacc
     8101 atccagaact cttgttccgt tcccaggtcg atattttctt tatcgccata ccagatgcgt
     8161 tccgttccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt ttgttgcccc
     8221 caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc ccccattatc
     8281 actgcaatga gacgatgctg cccatctaca gccgaagcaa tgagattaaa cccggcacca
     8341 gaagtatgac ccgttttcag gccgtcaaca ttcatggttt tatcccacaa taatccgtta
     8401 cggttttgct gggtgatacc gttccaggtg agacttttct cactgtacat atgataaaac
     8461 tcgggctcgc cgtggatgat agcacgagaa agcacagcta aatcataagc cgagctatgc
     8521 tggccaggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag atgcagcttc
     8581 tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat atagtcagcc
     8641 agagcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa atcacgtacc
     8701 gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac cggattatct
     8761 ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg atgactatcg
     8821 atagcgcgat ccacgacata acccgtcatc agctttgtca ggctggcggg attgcgctgt
     8881 tgatgctcat tacccgcggt gaggatctga ccggtggtgt aatccatcaa tacccaggac
     8941 ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc aaaaccagac
     9001 gataagttaa aaacgaacaa agaagcagca ataataagac ggcgtttcaa cagcaaaccc
     9061 tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc tggaataaag
     9121 tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatctgttgc cactcgctgc
     9181 caaattgtgg cgctaaagct gattagcacg gtgatatttg atactctggc agacagcaga
     9241 aataacggat ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac
     9301 gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt
     9361 cgcaccgata gcgaattcaa tgtcatcggc gaacccgaag tcttttactg caagcccgcg
     9421 gatgactatt taccccagcc tggagcagta ttaattaccg gtattacccc gcaggaagca
     9481 cgggcgaaag gagaaaacga agccgcgttt gccgcccgta ttcactcgct ttttaccgta
     9541 ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac
     9601 gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga taactcgcgc
     9661 tgggatttgt tggatgttat gcgtgcctgt tatgccctgc gcccggaagg aataaactgg
     9721 cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa agcgaatggt
     9781 attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat tgcgatggca
     9841 aagctggtaa aaacgcgtca gccacgcctg tttgattatc tctttaccca tcgtaataaa
     9901 cacaaactga tggcgttgat tgatgttccg cagatgaaac ccctggtgca cgtttccgga
     9961 atgtttggag catggcgcgg caataccagc tgggtggcac cgctggcgtg gcatcctgaa
    10021 aatcgcaatg ccgtaattat ggtggatttg gcaggagaca tttcgccatt actggaactg
    10081 gatagcgaca cattgcgcga gcgtttatat accgcaaaaa ccgatcttgg cgataacgcc
    10141 gccgttccgg ttaagctggt gcacatcaat aaatgtccgg tgctggccca ggcgaatacg
    10201 ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct cgataacctg
    10261 aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tggcgatatt cgcggaagcc
    10321 gaaccgttta cgccttcaga taacgtggat gcacagcttt ataacggctt tttcagtgac
    10381 gccgatcgcg cagcaatgaa aattgtgctg gaaaccgagc cgcgtaattt accggcactg
    10441 gatatcactt ttgttgataa acgaattgaa aaactgttgt tcaattatcg ggcacgcaac
    10501 ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg ccgccaggtc
    10561 ttcacgcccg agtttttaca aggttatgca gatgaattgc agatgctggc acaacaatat
    10621 gccgataaca aagaaaaagt ggcgctgtta aaagcgcttt ggcagtacgc ggaagagatc
    10681 gtctagtttg aagcctccgc cgctgatacg gcggaggaca atgttgggtc aggctttttg
    10741 aacggtgatg ctccacgcag catcgccaat ctgctgataa tcggtgatgg catgtccttc
    10801 ttctgccgcc cattgtggga ttgcttctgt tgcctgggtg caatcaaact caattaccaa
    10861 ctcatcgccg ctggccattc ctgccagtgc tgcttttgct tctatgagtg gaaacggaca
    10921 gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact taatttgccg
    10981 cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata cacaagccag gacgcagtcc
    11041 agactccgag aatcataaat acaaggccaa tccagccctg ccaggtcatc attgcagtca
    11101 taaccagtcc attaccgata gagcaaccac ctgcaatact ggcaccgaag cccatcagta
    11161 caccacctaa cccgctacgt aatgttgttt gtgcatcagc tgcgcgaacg cggaactcac
    11221 ggctcgcttt ggctgcaata aatgacccca cgaagatccc taacaccagg aaaacgcccc
    11281 agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc gttggggaag
    11341 tgattcctaa cccaaacatg cgtccggttg cttcactcag gggccaggct aaaagcgcaa
    11401 tcaaaccgat gagtacagca gtgacaaagg gatgccagcg tttttcaaac agaatatgag
    11461 cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc ttcaactctt
    11521 tcatcaccac ccagagcgtt atcaccagca aaacggcaac caacggccac acagacaaat
    11581 taaaagtctc agcaatagag ttatgttcag tactgtagtg ctgcaaggtt tgatttaaac
    11641 cactggcatg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc
    11701 aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat ccacccgcca
    11761 gaacaattcc cagcccaaag atatacccac ctataacagt accaagccac gggaacgccc
    11821 cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccaca ctttgtaccg
    11881 aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgagatac atatcgcgaa
    11941 aaccacctgt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa
    12001 ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg ttaataggga
    12061 aattattcat gactggtgga atgcagacca ataaccaaat tctctaaatt agaacaaatg
    12121 gttattaatg aggatgtcga taaaaaagaa taaaaaaacc ggagccaaat attcattcga
    12181 ctccggttta ttattacaaa agatgattac gctacgtctt cgtactgcgg aaccgggtta
    12241 cggaaactct tggtgacgca agccaggtaa atcagaccga tagccgccca gatcagaccc
    12301 agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag cgcaccacac
    12361 atcggcagga acagatactg gaagtgatct ttcagcgtct tattacgctt ctcacggatc
    12421 cagaactgcg agatgaccga caggttaacg aaggtgaacg ccaccaacgc accaaagtta
    12481 atcagcgctg tagccattac caggtcgaag ttgattgcca gcagggcaat cgcgccaacc
    12541 aggatgatgt tcatcgccgg agtacgccat ttcgggtgta cataaccgaa gaagcttttc
    12601 gggaatacgc cgtcacgacc cattacgtac atcagacgcg ctacgcctgc atgcgccgcc
    12661 ataccggacg ccagtacggt aatggtggag aagatcagcg cgccaacctg gaacgcttta
    12721 cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa gcgagagata
    12781 tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc gccaatcagc
    12841 gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc ttccgacagg
    12901 ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc
    12961 gggataacat gcgcatcgcc agaccagaac ggacgagtgc tcgccagcgt accagcgcct
    13021 tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag caccacctgc
    13081 aacacgacga ttacagtgtt gaagttcgct acggatttca gactacgcag gttaaaggcg
    13141 gtcatgaagg ccaccaacgc caccacaaac atccacgatg ggatggaagg caccagagct
    13201 tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata gtcgagcaga
    13261 gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc gtaagtgtat
    13321 gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc cgtaaacagg
    13381 atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag gcctgaaacg
    13441 ataccaaaag tatcaaacag cgtcatcggc tgcatatagg caagacccat cattacaacc
    13501 ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt aacgttatgc
    13561 gacatggtca ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt gccccatttt
    13621 tttggattcc tcagcgacaa caactgtcga tttttagtaa atatctatcc ggtacgaagc
    13681 ccggcctctt ggtatgaatg attggtttga agcaaaaaat aaccgacgct gatgaaacgt
    13741 cggtttttag tcattttttg acagcggcgc attgtgccta aatggggggg aaatgacaag
    13801 agaatgagag gcttgtcata ataatttttc tttaaatggc tgattttccg tcatcagatt
    13861 ttgcataaac accggcgatc gcactatttg ctaaaatttc atcccgccac cagccactgg
    13921 caagtcctgc ggtctgttca ttccagccaa tccagacggg ttcaaagaga gtttgggcga
    13981 ccactttctt ctcgattaac gcaccactat cgagaaaacg ttgcgccaga taacggggta
    14041 aatagccgca gccgaggccg ctaatttgca gctccagctt ggttttaaaa tcgaaaacgg
    14101 taatggcttc ttgttcgtcg agaagttgtg aggccgtaga agcgcctgcc tgcgcagtat
    14161 cccccaccac aatcgcccga tatcgcttaa tgatacgacg gtttaacggt tcttcttcca
    14221 gggctaacgg atgatgaggc gctaccgcaa agacttgttc cagatcgccc aaccgcgaga
    14281 aaccaaactc actggaagat ggtggctcat gcatcgcgcc aacaataata tccgctcgcc
    14341 cctgagtcag ggcatcccat gaaccaccga gtacgccatt gataaacttc aggcgcgtca
    14401 cgctatgatg ttgataaaaa gcttcaatca gtggcgcaag cagagaaaaa ggaaacgtgt
    14461 cgtccacgcc aatcaccagt tcgttttccc agccttcatg cagtttgatc gcctgctttt
    14521 ccagttctcg cacggtatgc agaacttccc gccctttctc taataacatt tttccggtgc
    14581 gggtgaattt agcgcggtgt ccgctacgat caagcaattg aatattaagg tcgctttcca
    14641 gcttgtgaac ggtgtaactc agagcggacg gcgtcttata gagtttggcc gacgccgccg
    14701 caaaactgcc ttctttttct aaggcatcga ggatcatcaa cacatccagc agtggtttca
    14761 tacacgcccc cttgcagtgc atgcggcgtc ctgctggcgt gatcgtttac tccagcggca
    14821 ttaccagcgg atcgggatac tggtattcaa atcccagttc attacaaatc cgactgccat
    14881 caataatctt acctttgccg ctgtccagac tatttctgaa ctgcggaggc tccagcccca
    14941 gtaaacgggc catctgcgga tagaaaacat tacgcgcagg gtgagctggc gcacatatat
    15001 tatagatgtg tccgccttta ggtgcctgta acaacagagt gatagcgcca ataacatctt
    15061 ctaaatggac taaattaaca ccatgttcac catcaggcgc ggtttttccg gcaaagaagc
    15121 gtccgggatg acgtcccggt ccgaccagcc ccgcaagacg cagaatatcg accgaagtac
    15181 cgggtaaatt gtgcagccag tcttcgagtt cttctaatac tcgtccactg ttggtcaccg
    15241 gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc gccatagaca gacgttgagc
    15301 tggtaaaaat aattcgggga atacgatggg ccagcgcgct atccactaac tcttgtaccg
    15361 cttgtaaata gaactcatcg ccggggccgc tacgacgtgc cggaagcgta atgaccagcg
    15421 catcggcatc catcagggca tccagatcgt cagaatcgca aactaactca ggctccatgc
    15481 gaagcagata gctgtcaatg ccactcattc gggccgcttc gacaccatct tgtgtggttt
    15541 tactcccggt gacttgccag cctcgcgctg aaagtgacat cgccagcggc atgcctaacc
    15601 accctaaccc gacaattgcg acctttttca tccgttatct cctgatacac cttagatcta
    15661 taaggctacg ctagcgtatc aaaactgaca attcattcta tgaatgaatc tgttcaataa
    15721 tgataacgac atgctgcaat gagcagtgaa tcgtcggtaa ccgcgtatac cagacggtgc
    15781 tcctctgtaa tgcgtcggga ccagaaacct gacaaattat gtttcagggg ttctggcttc
    15841 cccttacctt caaatggcgt tctgcgggta tctttgataa gttcattgat ctttttaaca
    15901 attcgcttat ctgtttcctg ccagtacaga taatcatccc atgattcctc agaccagatt
    15961 agtttcactc aataatgtcc ttttctgttc ctttgcctga tttcaggcta tcgattgagt
    16021 ccatcaatct ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgagt
    16081 tgtattcttc gagtgacatc agaacacaag cctctccatt ctgacgagta ataaggatcg
    16141 gggcatgatc ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt
    16201 agctaattgt acgcatgtca atctcctctt ttgtacagtt cattgtacaa tgatgggcgt
    16261 taattaacta tttattaatt agtttgtaga tcaaggtatt gtcagtgaga cgaaaatcca
    16321 ggcttcgcta tttttggtgc cgtcagctta gaggacagtc ctcttagccc cctcctttcc
    16381 ccgctcattc attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta
    16441 acgtgaaagt ggtttaggtt aaaagacatc agttgaataa acattcacag agacttttat
    16501 gatacgcgtt caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg
    16561 atgtgtgctg gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg
    16621 gaagatcacc ttccgggggc ttttttattg cgcggttgat aacggttcag acaggtttaa
    16681 agaggaataa caaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc
    16741 gtttaagtga tgactcacgc gaattgctgg cgcgctgtgg cattaaaatt aatcttcaca
    16801 cccagcgcct gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg
    16861 acgacattcc cggtctggta atggatggcg tggtagacct tgggattatt ggcgaaaacg
    16921 tgctggaaga agagctgctt aaccgccgcg cccagggtga agatccacgc tactttaccc
    16981 tgcgtcgtct ggatttcggc ggctgccgtc tttcgctggc aacgccagtt gatgaagcct
    17041 gggacggccc gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca
    17101 agcgttatct cgaccagaaa ggcatctctt ttaaatcctg cttactgaac ggttctgttg
    17161 aagtcgcccc gcgtgccgga ctggcggatg cgatttgcga tctggtttcc accggtgcca
    17221 cgctggaagc taacggcctg cgcgaagtcg aagttatcta tcgctcgaaa gcctgcctga
    17281 ttcaacgcga tggcgaaatg gaagaatcca aacagcaact gatcgacaaa ctgctgaccc
    17341 gtattcaggg tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg
    17401 aacgtctgga tgaagtcatc gccctgctgc caggtgccga acgcccaact attctgccgc
    17461 tggcgggtga ccaacagcgc gtagcgatgc acatggtcag cagcgaaacc ctgttctggg
    17521 aaaccatgga aaaactgaaa gcgctgggtg ccagttcaat tctggtgctg ccgattgaga
    17581 agatgatgga gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc
    17641 ggagcaacaa cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg
    17701 cactgttaac gatattctcg ataacgtgaa agcacgcggc gatgaggccc tgcgggaata
    17761 cagcgcgaag tttgataaaa ccacggttac cgcgctgaag gtgtctgctg aggagatcgc
    17821 cgccgccagc gaacgcctga gcgatgaact aaaacaggcg atggcggtgg cagttaagaa
    17881 tgttgaaacc ttccacactg cgcaaaaact gccgccgata gatgtagaaa cgcagccagg
    17941 cgtgcgttgc cagcaggtca cgcgcccggt cgcttcagtt gggttgtata ttcctggcgg
    18001 ctccgccccg ctcttctcaa cggtattaat gctggcgact ccggcgcgta ttgcgggctg
    18061 taaaaaagtg gtgctgtgct caccgccgcc gattgccgat gagatccttt atgcggcgca
    18121 gctgtgcggt gtgcaggacg tgtttaacat cggcggcgca caggccattg ccgcgctggc
    18181 gtttggtacg gaatctgtgc cgaaagtgga caaaatcttc gggccgggta acgcctttgt
    18241 caccgaagca aaacgccagg taagccagcg tctggacggt gcggcgatcg atatgcccgc
    18301 aggcccgtcg gaagtgctgg tgattgctga cagcggcgct acgccggatt tcgtggcttc
    18361 tgatttgctt tctcaggctg aacacggccc ggactcacag gtgattttac tgacgcccga
    18421 tactgatatg gcgcatcaag ttgccgaggc cgtcgaacgc caactggcag aactgccgcg
    18481 tgccgaaacc gcccgccagg cactgagcgc cagccgcctg atcgtgacta aagatttagc
    18541 gcagtgcgta gagatctcca accagtacgg cccggagcac ctgatcattc agacccgcaa
    18601 cgcccgcgaa ctggtcgatg gcatcaccag cgccggttcg gtatttcttg gtgactggtc
    18661 accggaatcg gcaggcgact atgcctccgg cactaaccat gttctaccga cttacggtta
    18721 caccgccacc tgttccagcc tcgggctggc agatttccag aagcgcatga ccgtacagga
    18781 actgtcgaaa gaggggttct ccgcgctggc ttcaaccata gaaacactgg ccgccgccga
    18841 gcgcctgacc gcccacaaaa atgccgttac tttgcgtgtt aacgccctta aggagcaagc
    18901 atgagcaccg tgactattac cgatttagcg cgtgaaaacg tccgcaacct gacgccgtat
    18961 cagtcggcgc gtcgtctggg cggtaatggc gacgtctggc tgaacgccaa cgaatatccc
    19021 acagccgtgg agtttcagct tactcagcaa acgctcaacc gctacccgga atgtcagccg
    19081 aaagcggtga ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc
    19141 agccgtggcg cggacgaagg tattgaactg ctgatccgcg ctttttgcga accgggtaaa
    19201 gactccatcc tctactgccc accaacgtac ggcatgtaca gcgtcagcgc cgaaacgatt
    19261 ggcgtcgagt gccgcacagt gccgacgctg gacaactggc aactggactt acagggcatt
    19321 tccgacaagc tggacggcgt aaaagtggtc tatgtttgca gccccaacaa cccgaccggg
    19381 caactgatca atccgcagga ttttcgcacc ctgctggagt taacccgcgg taaggcgatt
    19441 gtggttgccg atgaagccta tatagagttt tgtccacagg catcgctggc tggctggctg
    19501 gcggaatatc cgcacctggc tattttgcgc acactgtcga aagcttttgc tctggcgggc
    19561 cttcgttgcg ggtttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc
    19621 gccccctacc cgctctcgac gccggttgcc gacattgcgg cccaggcgtt aagcccgcag
    19681 ggaatcgtcg ccatgcgcga acgggtagcg caaattattg cagaacgcga atacctgatt
    19741 gccgcactga aagagatccc ctgcgtggag caggtttttg actctgaaac caactacatt
    19801 ctggcgcgct ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca gggcattatc
    19861 ttacgtgatc agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc
    19921 cgtgaagaaa gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga
    19981 agtatctttt tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg
    20041 tggaccgttt tgacaaactc gcctttgaac cgggcgtgat cccggagctg ctaaagttgc
    20101 aaaaagcggg ctacaaactg gtgatgatca ctaatcagga tggtctggga acacaaagtt
    20161 tcccgcaggc ggatttcgat ggcccgcaca acctgatgat gcagatcttc acctcgcaag
    20221 gcgtgcagtt tgatgaagtg ctgatttgtc cgcatctgcc cgccgatgag tgcgactgcc
    20281 gtaagccgaa agtaaaactg gtggagcgtt atctggctga gcaagcgatg gatcgtgcca
    20341 acagttatgt gattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggtatta
    20401 atggtttacg ctacgaccgc gaaatcctga gctggccgat gattggcgag caactcacta
    20461 aacgagaccg ttacgcccat gtagtgcgca acaccaaaga gacgcaaatt gacgtccagg
    20521 tgtggctgga tcgtgaaggt ggcagcaaga ttaataccgg cgttggcttc tttgatcaca
    20581 tgctggatca gatcgccacc cacggcggtt tccgcatgga aatcaacgtc aaaggcgacc
    20641 tctatatcga cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa
    20701 aaattgccct cggcgataaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg
    20761 aatgccttgc ccgctgtgca ctggatatct ctggtcgccc gcacctggaa tataaagccg
    20821 aatttaccta ccagcgcgtg ggcgatctca gcaccgagat gatcgagcac ttcttccgtt
    20881 cactctctta caccatgggc atgacgttgc acctgaaaac taaaggtaaa aacgatcacc
    20941 accgtgtaga gagcctgttc aaagcctttg gtcgcaccct gcgccaggcc atccgcgtgg
    21001 aaggcgatac cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac
    21061 cggctgcgcc aacctgaact cggtgaagtc tgccattgcg cgtcacggtt atgaacccaa
    21121 agtcagccgt gacccggacg tcgtgttgct ggccgataaa ctgtttttac ccggcgttgg
    21181 cactgcgcaa gcggcgatgg atcaggtacg tgagcgcgag ctgtttgatc tcatcaaagc
    21241 ctgtacccaa ccggtgctgg gcatctgctt agggatgcaa ctgctggggc ggcgcagcga
    21301 agagagcaac ggcgtcgact tgctgggcat catcgacgaa gacgtgccga aaatgaccga
    21361 ctttggtctg ccactgccac atatgggctg gaaccgcgtt tacccgcagg caggcaaccg
    21421 cctgtttcag gggattgaag acggcgcgta cttttacttt gttcacagct acgcaatgcc
    21481 ggtcaatccg tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca
    21541 aaaagataac ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggtgctaa
    21601 gttgctgaaa aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc
    21661 actgtggtgc gtctccatca gggcgattac ggcaaacaac gcgattacgg taacgacccg
    21721 ctgccgcgat tgcaggatta cgccgcgcag ggtgccgaag tgctgcacct ggtggatctg
    21781 accggggcaa aagatccggc taaacgtcaa atcccgctga ttaaaaccct ggtcgcgggc
    21841 gttaacgttc cggtgcaggt tggcggcggc gtgcgtagcg aagaagatgt ggcggcgtta
    21901 ctggaagcgg gcgttgcgcg cgtagtggtc ggctccaccg cggtgaaatc acaagatatg
    21961 gtgaaaggct ggtttgaacg cttcggtgcc gatgccttag tgctggcgct ggatgtccgt
    22021 attgacgagc aaggcaacaa gcaggtggca gtcagcggct ggcaagagaa ctcgggcgtt
    22081 tcactggaac aactggtgga aacctatctg cccgtcggcc tgaaacatgt gctgtgtacc
    22141 gatatctcgc gcgacggcac gctggcaggc tctaacgtct ctttatatga agaagtgtgc
    22201 gccagatatc cgcaggtggc atttcagtcc tccggcggta ttggcgacat tgatgatgtg
    22261 gcggccctgc gtggcactgg tgtgcgcggc gtaatagttg gtcgggcatt actggaaggt
    22321 aaattcaccg tgaaggaggc catcgcatgc tggcaaaacg cataattccg tgtctcgacg
    22381 ttcgtgatgg tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata
    22441 tcgtgccgct ggcaaaacgc tacgccgaag aaggtgcaga cgaactggtg ttctacgata
    22501 tcaccgcttc cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag
    22561 tgatcgacat tccgttctgt gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa
    22621 ttctttcctt tggcgcggat aaaatttcca tcaactcgcc tgcgctggcg gacccgacgt
    22681 taattactcg cctggccgat cgctttggcg tgcagtgtat tgtggtcggt attgatacct
    22741 ggtacgacgc tgaaaccggt aaatatcatg tgaatcaata taccggcgat gaaagccgca
    22801 cccgcgtcac tcagtgggaa acgctcgact gggtagagga agtgcaaaaa cgcggtgccg
    22861 gagaaatcgc cctcaacatg atgaatcagg acggcgtgcg taacggttac gacctcgagc
    22921 agctgaaaaa agtgcgtgaa gtttgccacg tcccgctgat tgcctccggt ggcgcgggca
    22981 ccatggaaca cttcctcgaa gccttccgcg atgccgacgt tgacggcgcg ctggcagctt
    23041 ccgtattcca caaacaaata atcaatattg gtgaattgaa agcgtacctg gcaacacagg
    23101 gcgtggagat caggatatgt taacagaaca acaacgtcgc aaactggact gggaaaaaac
    23161 cgacggactg atgccggtga ttgtgcaaca cgcggtatcc ggtgaagtgt taatgctggg
    23221 ctatatgaac ccggaagctt tagacaagac catcgaaagc ggcaaagtca ccttcttctc
    23281 gcgcactaaa cagcgactgt ggaccaaagg cgaaacgtcg ggcaatttcc tcaacgtagt
    23341 aaatattgcg ccggactgcg acaacgacac gttactgttg ctggcgaatc ccatcggccc
    23401 gacctgccac aaaggcacca gcagctgctt cggcgacacc gctcaccagt ggctgttcct
    23461 gtatcaactg gaacaactgc tcgccgaacg caaatctgcc gacccggaaa cctcttacac
    23521 tgccaaactg tatgccagcg gcaccaaacg cattgcgcag aaagtgggcg aagaaggcgt
    23581 ggaaaccgca ctggccgcga ctgtacatga ccgctttgag ctgaccaacg aggcatctga
    23641 tttgatgtat cacctgctgg tgttgttgca ggatcaggat ctggatttaa caacggtgat
    23701 tgagaacctg cgtaaacggc atcagtgagt tgcggggtaa gcggatgcga tgttgttgtc
    23761 gcatccggca aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
    23821 aagattactt cgcgttgtaa ttacgcagag cgttacgccc cagcacaatc cccgcaccaa
    23881 ccatgccgcc cagcaatacc gccagaatca aagtgattgc ctttttcggg ctatcgcgac
    23941 gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga tcatcaactt
    24001 ttaagctttc gatatccagc aggttttgac gagtctgata gtagtttggc gagaacacca
    24061 acggacgggt cgcctcatgc ttaatcatcg actccagcgc ttcgctcccc aaaaggaaca
    24121 acgtatcttg cgtgatatct tcgccagtct gttgaatttg cggttttgtc acctgcgcct
    24181 gattcgcata ctgcaacgct tcctgaatct gacggatacg cagatctttc tgctcttgcg
    24241 cgactacttc ctgagttctt aaagagtcct gcaagttttt catacgcagc gcgatgttgt
    24301 ctttcaagtc tttttccagc tctacgttta cctggtcgtc aacttgctgg atatacttcg
    24361 ccagctgctt ctgaacacct tcaggtgttt caccaacata agacaccgct aaaggtaatg
    24421 actgattttt aacggtaggc tcaatggtca gcttttctgc ttcttcctgg ttatccagcg
    24481 tttccgctaa tgctgagaat gcagtgctgt aacgtccgat aagtgaagcc tgaatgtcgg
    24541 agactttcgg tacagccgga ccgtaaatca cattcatcgc gttggtataa ccagcgattt
    24601 gccccgcatc aggttgagta acgatagcag tggaagtcca cttttctttg gcaacggcca
    24661 gataccctat agccagggca atagccacaa tgacagaaat gatgatagtc atcttgccgc
    24721 gccacaactg caccagtaaa tcaatcaaat caatctgttc cgggtcatgg ttttgcccag
    24781 aaacattatt attttctact ctcatcatta ccctaacgga cgaaataatt ttaagtcgcc
    24841 atagtttagc gataatcgcc atgaatgcta taggataaat gataaaaata atgaattatt
    24901 acaaagggaa cataagagaa tcagggcgaa aatcgccctg ataacaggat gttagtcgct
    24961 gccaaagaga tcgcgggtgt atactttgtc tgccacatcc ttaagctctt ctgccatacg
    25021 gttggaaata atgacatcgg cttgttgttt gaaagtggcg agatcacgtt ccagccgaga
    25081 gttgaagaat gaatcttctt tcatcaccgg ttcgtagatg atcacttcaa cgcctttcgc
    25141 cttgatacgt ttcataatcc cctgaatgga agacgcacgg aagttatctg atccgctctt
    25201 cataatcaga cgataaatac ccaccacttg cggcttgcgt gacaaaatgg catcggcaat
    25261 aaagtcttta cgcgtgcggt tagcatcgac aattgccgag atcaggttat tcggcacaga
    25321 ctggtagttc gccagtaact gcttggtatc tttcggcaga caataaccac cataaccaaa
    25381 cgacgggttg ttgtaatggt tgccaatacg cggatcgaga caaacgcctt cgataatctg
    25441 gcgagtattc agccctaaac tttctgcata gctatccagt tcattaaagt atgctacgcg
    25501 catcgccaga taggtattag cgaaaagttt aatcgcttct gcttcagtgg agtcggtaaa
    25561 cagggtcggg atattttgct taatcgcgcc ttcctgtaat aacgccgcaa aacgttctgc
    25621 gcgttctgaa cgctcaccga tgacaatacg tgaaggatgg agattatcgt aaagggcttt
    25681 accttctcgg aggaattccg gagagaaaat aatattttca gtacgatatt tcttatgcat
    25741 cgcttcggta aaaccaacgg gaaccgttga tttgatgacc ataaccgcat aaggatttat
    25801 ctcaactacg tctttaatta ctgattctac actggatgta ttgaaataat tagttttagg
    25861 atcatagtcg gttggagtgg cgatgatgac ataattagca ccccggtagg cttcattttt
    25921 atctaatgtg gcattaaagt gtattttatc tgattgcaaa aaatgctgaa tttccttatc
    25981 aacaatagga gatatccgat cattcagcat agcaacgcgt gacggtaaaa tatctaatgc
    26041 cacaacctca tgattttgtg cgattagaag cccgtttgac aagcctacat agccggtacc
    26101 ggaaatggtg attttcattt catgctctca gaattaactt aactgtgaat catgatgttt
    26161 ttagcatcct gataagagct aaaagtttta acgctacgtt gtcagatggt caacgcaacg
    26221 aaaaacaatt cagattttct ttataagaat agctgatatt tattaagtta atattaagca
    26281 gtgaaattta gtctataaga taaatggcaa aaaaaagccc ggtgcaatat acgccgggcc
    26341 tcaattttat tgttggttaa atcagattaa tccagccatt cagtatggaa cacaccttct
    26401 ttatcaatgc gcttataagt atgtgcaccg aaatagtcac gctgtgcctg gatcaggttt
    26461 gcaggcagaa ctgcggcgcg gtagctgtca taataggcaa ccgcagccgc gaaggtcgga
    26521 accgggatac cgttctgtac tgcataagca acgacatcac gcagcgcctg ctggtagtca
    26581 tcggcaattt gcttgaagta cggagccagc agcaggttag cgatctgcgg attttcggca
    26641 taagcatcgg tgattttctg caggaactgc gcacggatga tgcagccagc acggaaaatt
    26701 ttcgcgattt cgccgtagtt cagatcccag ttgtactctt cagacgccgc tcgcagctga
    26761 gagaagccct gagcgtaaga aacgattttg cccagataca gagcacgacg aactttttcg
    26821 atgaactcag ctttgtcgcc tgctggctgc gcttgcggac cagagagaac tttagacgcg
    26881 gcaacacgct gatctttcag agaagagata taacgtgcaa acacagattc ggtaatcagc
    26941 gacagcggtt cgccaagatc cagcgcactc tggctggtcc atttgcccgt acctttgttt
    27001 gctgcttcat ccaggatcac atcaaccaga tagttaccgt cttcatcttt tttggtgaag
    27061 atatctttgg tgatgtcgat caggtagctg ctcagttcac cgttattcca ctcggtaaag
    27121 gtctgcgcca gttcttcgtt ggtgaggttc agaccacctt taagcagaga ataggcttca
    27181 gcaatcagct gcatatcgcc gtattcaata ccgttgtgaa ccatcttcac atagtgacct
    27241 gcgccatcgg caccaatata ggtaacgcat ggctcaccgt cttcagccac tgcggcgatt
    27301 ttggtcagga tcggcgcaac aagttcatag gcttctttct gcccaccagg cataatggaa
    27361 ggacctttta gtgcgccctc ctcaccaccg gaaacaccgg taccgatgaa gttaaagcct
    27421 tctgcggaaa gctcacggtt acgacgaatg gtgtcctgga agaaggtgtt accaccatcg
    27481 atgatgatgt cgcctttatc gagatatggt ttgagggaat caatagcagc atccgtgcct
    27541 gcacctgctt tcaccattaa caggatgcga cgaggcgttt ccagagattc aacaaactct
    27601 ttcaccgtat agtaaggaac cagtttcttg cccggatttt cggcaatcac ttcttccgtc
    27661 ttttcacggg aacggttgaa aatagagacg gtataaccac ggctttcgat gttgagcgca
    27721 aggttgcgcc ccatcactgc cataccgact acgccaatct gttgctttga cattgtttac
    27781 tcctgtcagg ggtggtcacc gcgacgtatg cacggcttga atgtagtgta gat
//
LOCUS       DAZFYC010000063        27771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFYC010000063 DAZFYC010000000
VERSION     DAZFYC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.063"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..315
                     /gene="yhcN"
                     /locus_tag="ACHKW5_002894"
     CDS             52..315
                     /gene="yhcN"
                     /locus_tag="ACHKW5_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO7512250.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(370..642)
                     /gene="yhcO"
                     /locus_tag="ACHKW5_002895"
     CDS             complement(370..642)
                     /gene="yhcO"
                     /locus_tag="ACHKW5_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO7512251.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(734..2701)
                     /gene="aaeB"
                     /locus_tag="ACHKW5_002896"
     CDS             complement(734..2701)
                     /gene="aaeB"
                     /locus_tag="ACHKW5_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO7512252.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSTLGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(2707..3639)
                     /gene="aaeA"
                     /locus_tag="ACHKW5_002897"
     CDS             complement(2707..3639)
                     /gene="aaeA"
                     /locus_tag="ACHKW5_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO7512253.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3647..3850)
                     /gene="aaeX"
                     /locus_tag="ACHKW5_002898"
     CDS             complement(3647..3850)
                     /gene="aaeX"
                     /locus_tag="ACHKW5_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO7512254.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4033..4962
                     /gene="aaeR"
                     /locus_tag="ACHKW5_002899"
     CDS             4033..4962
                     /gene="aaeR"
                     /locus_tag="ACHKW5_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO7512255.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5090..6535)
                     /gene="tldD"
                     /locus_tag="ACHKW5_002900"
     CDS             complement(5090..6535)
                     /gene="tldD"
                     /locus_tag="ACHKW5_002900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO7512256.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6691..10491)
                     /gene="yhdP"
                     /locus_tag="ACHKW5_002901"
     CDS             complement(6691..10491)
                     /gene="yhdP"
                     /locus_tag="ACHKW5_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO7512257.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10559..12028)
                     /gene="rng"
                     /locus_tag="ACHKW5_002902"
     CDS             complement(10559..12028)
                     /gene="rng"
                     /locus_tag="ACHKW5_002902"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO7512258.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12018..12611)
                     /gene="yhdE"
                     /locus_tag="ACHKW5_002903"
     CDS             complement(12018..12611)
                     /gene="yhdE"
                     /locus_tag="ACHKW5_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO7512259.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12620..13108)
                     /gene="mreD"
                     /locus_tag="ACHKW5_002904"
     CDS             complement(12620..13108)
                     /gene="mreD"
                     /locus_tag="ACHKW5_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO7512260.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13108..14211)
                     /gene="mreC"
                     /locus_tag="ACHKW5_002905"
     CDS             complement(13108..14211)
                     /gene="mreC"
                     /locus_tag="ACHKW5_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO7512261.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPVNTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14277..15320)
                     /gene="mreB"
                     /locus_tag="ACHKW5_002906"
     CDS             complement(14277..15320)
                     /gene="mreB"
                     /locus_tag="ACHKW5_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO7512262.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15625..17565)
                     /gene="csrD"
                     /locus_tag="ACHKW5_002907"
     CDS             complement(15625..17565)
                     /gene="csrD"
                     /locus_tag="ACHKW5_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO7512263.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17717..18691
                     /gene="yhdH"
                     /locus_tag="ACHKW5_002908"
     CDS             17717..18691
                     /gene="yhdH"
                     /locus_tag="ACHKW5_002908"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO7512264.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            19669..20139
                     /gene="accB"
                     /locus_tag="ACHKW5_002909"
     CDS             19669..20139
                     /gene="accB"
                     /locus_tag="ACHKW5_002909"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO7512265.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20150..21499
                     /gene="accC"
                     /locus_tag="ACHKW5_002910"
     CDS             20150..21499
                     /gene="accC"
                     /locus_tag="ACHKW5_002910"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO7512266.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            21608..21850
                     /gene="yhdT"
                     /locus_tag="ACHKW5_002911"
     CDS             21608..21850
                     /gene="yhdT"
                     /locus_tag="ACHKW5_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO7512267.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21840..23291
                     /gene="panF"
                     /locus_tag="ACHKW5_002912"
     CDS             21840..23291
                     /gene="panF"
                     /locus_tag="ACHKW5_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO7512268.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLSPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            23303..24184
                     /gene="prmA"
                     /locus_tag="ACHKW5_002913"
     CDS             23303..24184
                     /gene="prmA"
                     /locus_tag="ACHKW5_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO7512269.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            24513..25478
                     /gene="dusB"
                     /locus_tag="ACHKW5_002914"
     CDS             24513..25478
                     /gene="dusB"
                     /locus_tag="ACHKW5_002914"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO7512270.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            25504..25800
                     /gene="fis"
                     /locus_tag="ACHKW5_002915"
     CDS             25504..25800
                     /gene="fis"
                     /locus_tag="ACHKW5_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO7512271.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            25886..26770
                     /gene="yhdJ"
                     /locus_tag="ACHKW5_002916"
     CDS             25886..26770
                     /gene="yhdJ"
                     /locus_tag="ACHKW5_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7512272.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            26854..27033
                     /gene="yhdU"
                     /locus_tag="ACHKW5_002917"
     CDS             26854..27033
                     /gene="yhdU"
                     /locus_tag="ACHKW5_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO7512273.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRI"
     gene            complement(27036..27698)
                     /gene="acrS"
                     /locus_tag="ACHKW5_002918"
     CDS             complement(27036..27698)
                     /gene="acrS"
                     /locus_tag="ACHKW5_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO7512274.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         6774 a         7387 c         6950 g         6660 t
ORIGIN      
        1 atgggtcacg aaacaaaggc ccagctaaaa gattatgtcg aggtaaaaat catgaaaatc
       61 aaaaccactg tagctgcatt aagcgtactt tctgttctct ctttcggtgc attcgctgcc
      121 gactccattg atgctgcaca agcacaaaat cgtgaagcaa tcgggaccgt atccgtaagt
      181 ggtgtggcgt cttcgccaat ggatatgcgt gaaatgctga acaaaaaagc ggaagagaaa
      241 ggcgcaacgg cctaccagat tactgaagct cgtagcggtg acacctggca cgctacggct
      301 gaactgtaca aataaaccct catcgtcttg tccgacgata ttgcccccgg ttcgggggct
      361 ttttttgcgc taatgacgaa cattaaaacg caaatgccct tccagctctt cctctgcctc
      421 atcaaacagc aatattaacg cgccaaaacg gcgacgcgtt ttctctccca gatgaacaaa
      481 ttcaatctca agtggtagcg gcaggacatc gttcattaac acatcccata gtgagtcgag
      541 atcgcgtacc ttatctttcg ccagaccaaa ggtttggcta aagtcacgat aaaaatcctc
      601 ctgactctca atctcatcaa aatcaaaggt ataaatattc atctgttgcc accgtcacgt
      661 ttcgcggcga gttctgccgt gcatcgctaa gtatagccat gaaaaaaccg acgcttttgg
      721 cgtcggtttt gacttaacta tcggtcaacg catgttgata cttatggagc atccccgcca
      781 gccgatgaac cggttccgtc acctgcggtg gcgcttgcca gatgcgtagc ttttcctggt
      841 agatttccag ttcttccagc aactggccaa agtaccgacg acgtttatca tcgctacggg
      901 cagatatcac atggtctgct gtgcggcgca tttgtcggtg aaacgccgat aaatcctcgt
      961 taaccgggat cggcgcatca cgcaggcgct ggtgcgcgat aatcatcgtc agcgccaggc
     1021 gaaatttcgg caaatcccct gggaacttat tcatcagcaa aaacagctgc tgataaagtg
     1081 ccgggaggtg gttctcttta cgacgtgcca cattggtagt catcgcggaa acagcggcag
     1141 aaacaaactg attaagcagt acacgtccag tcctgtcgcg cgatttatcc cgcaccagca
     1201 aaataacggt gaacgcgagc acacagccga cgatttgccc taatgtgctg tcgagaaact
     1261 gactgaaatg gaaagtcatc gggttatcca gcacgataat atttatggtg ctggccagcg
     1321 cccccatcga gcccagtcgc cgtttctgta cttctattcc gaggaagaat cccagcactg
     1381 ccaggctaat acacagcagc aacatgctct gttgggtatt agggataatc accaaaaagt
     1441 agagcagccc taacggcagc gcggccagcg tcccgtagat aaagtcgatc gccaccatgc
     1501 gtggattcgg caaacgcatt gccagtgacg tcacgaccgc aatcatcacc attgcaccac
     1561 tgccggaagt ccagcccgtc cacagccaga aaagcgttcc cagaatgcag gaaagtgtgg
     1621 ttcgccagaa gttaaccatc gcatgatgac gttcggctga ctctactttg acttccggtt
     1681 cgccttgcag gatctcttct tcggtggcgt tgatttttgt gttactgata acgccgcgct
     1741 tgagaagctg ataacgcgtt gccgccgcga cccagctata aatggtgaca ggcgtttccc
     1801 gttccccggt ccaggcgata actctccgca ggcgtttgag ctgcttgtgg acgtcctgcg
     1861 cggtttctac cggcgtgtca aaaaattcgc ggaaagtatc agtgatcaat tccgggcgcg
     1921 tattctgaat aagataagtt tcgcaggatt gggtaatcag cgtcagcgat agcgtattga
     1981 tcgcttttaa acgtcgattg gcccgcgccc agcgggaaga ttccatattc aggttgctgc
     2041 gcatgccttg tagcgccgtg gtgcgtcgca ccaggtcgcc ccaggcttta tcgacaactt
     2101 caccatcgcc atgcttgata cagagttgca ttaattgata ttgcgcgacc agcaaacttt
     2161 ccagctctcg atccacttct tgtttgatcg atcgcggaga aaagagcaaa tccgccataa
     2221 tcgcacacac aataccgata acgatctcgc tacaacgttc gacggcaaac tgcggcgtaa
     2281 gcaatggttc cggctgaatg gtgatcacaa tgatcagagc ggtataaccg gccagccccc
     2341 acgcgtacga gttttctatt cgtaccagcg aggatatcca ggtacaaaaa ccggcccaga
     2401 tacagcacac cagaatcatc aataatggtg cgcggatcat cgcaatgatg atcaccagtc
     2461 cggcaataca gccaataaat gtgccgatga tgcgcaaaaa gccacgatag cgaatagcgc
     2521 cagaatacgg ttcacctccc gcagcaaagg ccggaccggc ggcaacaatc gccgctgtca
     2581 gtaccgccca gcgtggcgtt tccagctgga agtgaaagcc aacaaacagc gccagtacaa
     2641 tggcggtcgc cagttttacc gcaaagcgaa tatgttggtt agcaatggag aaaataccca
     2701 tcgtgattaa ccaaactcac gcaggcgatg ggccatttta cggaagaacg aatcctggct
     2761 ttcgtcgcga tcttgtttgc cagtgaccac cactgtagca gtggtgcccg caggccagat
     2821 gttctcttgc tggttgtcga gacgaatacg aaccggaaca cgttgcgcca aacgcaccca
     2881 ttcaaggtta gagtcgatgg tcgccatccc tttgtcgtca cgcgtgctgc tggcgttggt
     2941 gaccccagcg gcaacactat caacagtccc tttcagcact ttgttactgc caagcggcgt
     3001 gatctctgca cgataccccg gacgcacccc ttccagctta gtttcttcca tataggccag
     3061 tacatagaag gagttctgtt tcaccagcgc aaccgccgtt gatcctcgag taataaactc
     3121 accggtatag acgttgaggt tggtcaccca gccatctgct ggcgcgcgga tcaccgtgcg
     3181 ttcaagatcc agttttgcca gatcgcgggt cgcctgcgct ttcgctaact gatgcagaac
     3241 cgtttgtagt acgttgttag cctggtcgat ctcttcgcga gacatcgcct gcacaccgag
     3301 acggttacga cgtccggcct cctggcgttt ctcctgtgcc agtacctgat aataagcaac
     3361 atcggcttgc gcttcctcaa gcgccttttg atagcgcggc tggtcgatgg tgaacagtat
     3421 ctgtcctttt ttcaccagct ggttatcatg aacattcacc tgggtaatga gtccagaaac
     3481 gtccggcgcg atcgcaacga cgtcagcgct aaagcgcgcg tcacgcgtcc agggggattc
     3541 ggtgtaatag acccaggcat taaaaattgc gatgaaggcc agaatgacta atacgaccgt
     3601 gatggccgta cgggagaatt ttcttattag tgttttcact tcaacctcaa acgaacagtc
     3661 gcgatatcaa ataaaacaag cagcaataga gcgcggtgtt gaacaacgcc ggatgccaga
     3721 caaagtcgta gatacctgtt ggcacaagta cccggcgcac cagccagaaa atcgccagtg
     3781 ataaaagcaa ttcaaaaaat atcggtggga aggacagccc aaacaccacg ataacgggaa
     3841 acagactcat gttgaccttg gttgtataga gagagcaggc gttattattt tcagcatctg
     3901 tcgccgcaga gaagggcatg gaaagccggg cgagagcaac attgctgtag attgatattt
     3961 aatatattag cgtaactgtt atgctgttat ctatattatg tgatctaaat cacttttaag
     4021 tcagagtgaa taatggaacg actaaaacgc atgtcggtgt ttgccaaagt agttgaattt
     4081 ggctctttta ccgccgccgc cagacagcta cagatgagcg tttcgtccat cagtcagacg
     4141 gtgtcaaaac tggaagatga gttgcaggta aagctgttaa accgtagcac acgcagcatt
     4201 ggcctgaccg aagccggtag aatttactac cagggctgcc gtcgtatgct tcatgaagtg
     4261 caggatgttc atgagcaact gtatgccttc aataacaccc ccatcgggac gctacgcatt
     4321 ggctgttctt caactatggc acaaaatgtt ctcgccgggc tgacagccaa aatgctgaaa
     4381 gaatacccag gtttgagcgt caatctggtt accggaattc cagcccccga cctgattgcc
     4441 gacggtctgg atgtggtgat ccgcgtcggc gcgttgcagg attccagcct gttttcccgc
     4501 cgtcttggcg cgatgccaat ggtggtgtgc gccgcgaaaa gctatctcac acaatacggc
     4561 ataccggaaa aacccgccga tttaagtagc cattcatggc ttgaatacag cgtgcggccc
     4621 gacaatgaat ttgaactgat tgcaccggaa gggatctcga ctcgcctgat cccgcaaggg
     4681 agatttgtga ctaatgatcc gatgacgctg gtgcgctggc taacggcggg tgccgggatc
     4741 gcctacgtgc cgctgatgtg ggtgatcaac gagatcaatc gcggtgagct ggagatcctg
     4801 ctgccgcgtt atcagtcaga tccacgcccg gtttatgcgt tatataccga aaaagataag
     4861 ctgccgctga aggtacaggt cgtgatcaac tcgctgacgg attattttgt tgaggtcggt
     4921 aaattgtttc aggagatgca cgggcgcggg aaagagaagt aattcatatt gtactgttac
     4981 gttctacaaa cctgtgccaa cgggttcccc tcaccctaac cccagaggag cgaggggacc
     5041 gttcgtgcac gtagaaagat taattatcct tctgaaaata atgaaattat tacgcagtac
     5101 cgccaacagt caggttatca actttcagcg ttggctggcc cacgccaacc ggcaaacttt
     5161 gcccttcttt accgcagaca cccacgccgt tatccagttt caggtcgttg ccaaccatcg
     5221 aaatctgctg catggtttcg ataccggaac caatcaacgt cgcgcctttc accggcttcg
     5281 ttactttacc gttttcaatc agatacgctt ctgaagtgga gaaaacgaat ttgccggatg
     5341 taatatccac ctgaccgcca ccaaagttcg gtgcatagat accgtactca acggattcaa
     5401 taatttcctg cggggtcgat ttacccggca gcatataggt gttggtcata cgcggcatgg
     5461 gcagatgggc gtaggattca cggcgaccgt tgccagtcgg cgtcatcccc atcaaacgcg
     5521 cgttgagttt atcctgcatg tagcctttca gaatgccgtt ctcaatcagc acgttgtact
     5581 ggcctggcgt accttcgtca tcaatcgcca ccgaaccacg gcgatcgacc atcgtgccgt
     5641 catcaaccac ggtacacagt tctgaagcca ccagctcccc gacctgccca ctaaatactg
     5701 aagtgccgcg gcggttgaag tcgccttcca gaccgtgacc taccgcttca tgcaacagca
     5761 cgcccggcca acctgcgcca agtactaccg gcatggtgcc cgctggtgca gcaacggcag
     5821 aaagattgac cagcgccata cgcactgctt cttttgccca tgcatccgca cggacttcgc
     5881 cgtcaagatc ggcaaggaag aactcataac caaaacgacc gccgccgcca ctggcaccgc
     5941 gttcgcgttt gccatcttct tcgaccagaa cgctcacgga aagacgcacc agcggacgaa
     6001 catccgccgc cagcgtgccg tcggtggccg caaccagaat taattcataa acgccgctga
     6061 ggctggcagt cacttcttgt acgcgcttgt ccgcttcgcg ggcaacctta tcgacgcgac
     6121 gcaggatatc cagcttctct tcacggctca tgctttgcag cggatctacc gaggtataca
     6181 acgggctatg ctctaccgcg cccagcgtct gtactttgcc atcaccacta tcacggacga
     6241 tggtgcgcgc cgcttgcgca ctctgttcca gcgccagcag gctgatttgg tcagcgtaag
     6301 caaatccggt tttttcacca ctgattgcac gcacaccaac gccctgatcg atgttgtaag
     6361 aaccatcttt aataatgcgg tcttctaaaa cccaggattc gtgatagctc gactgaaaat
     6421 agagatcgcc ataatcaagg cgacgttcgg ccagttgacc gaggatcgcg aacaagtcct
     6481 gatgtttcag gccgttcgcc gctagcaatt gttcacttac caggttcaga ctcatcgttt
     6541 ttgctactcg ttagttactg cagtagagga ttttttacgg ctgccggatg cggcgtgaac
     6601 gccctatccg gcctacagtt atgttccggc tcgttggcct ttggcaacga ttatcatatg
     6661 agattggggc aattacgcgc cctcgtcaaa tcattgcgct ttttctttac gcggttggcg
     6721 caacacttcg ttgatttgcg gatcgtccag cggacccgaa atgtgatagc gcaaaatgga
     6781 gactttgctc cacagcggcc ccagcacttt actggctgca aatactgccg cgccaacaat
     6841 ggggttaacc gcaaaagccg cagccacgcc caccgtcgca gaaatctctg gtgcgacaac
     6901 cgcttccata ttcaggtcgc gacgtaccag atttaccgac cctttcatgg cgatatccgc
     6961 ctccaggcca tccaccagcg tgtcgtcggt gtgcataacc ccgtctttaa tccacgcggt
     7021 gctgcgaatg gagtcaaaat agaacccttc gccaaaagtg tctctgaaat caaaacgcag
     7081 cttacgcatc agggcatcaa cgctcaataa gcgcagcaat tgcccggcat gtccggtatt
     7141 gatttcggta atttcgcctt tacccagttg agtatgaatg atgccattca acgtcgcctc
     7201 atctggttgc cagggtgctt tgcgccagtg taaatcgtaa tccacattaa atgacgactg
     7261 gcgtatgggc gtcgtgacac caaaaaattc tgcggcggca tcaattttct gcccgcgcag
     7321 ttttcctttc agcgaggtac gttcattctc cggattatta acccattcac catcggcagt
     7381 aagccgcgag aaaccagtat caatcagtcc attggtcagc gttaacgtat cgccagaaat
     7441 ggtgatatca ctgtcaatgc gaccgaattt ttgcccccag aaccagcact ctgtgcatcg
     7501 tatttgtgcg tccggccagc cacggaagtt aatgcgctcc gttgtcggga aaggtgatga
     7561 cggcgttgaa tcaccacgag ttttcgccac gctcgggttg taataaagat atttgatatt
     7621 cgccagccac ggcgcgttat tacgcatcgc tagcgtggcg ttgatttcac gcccttgcgc
     7681 ctcaaccagg gtgccatttg ccgtcggttg cgaaacaata ctcaggttat tccattgctg
     7741 atttcccagt gacaacatag gcgtacgtaa cgttatgtgt tgtgggaaac tcgctgcacc
     7801 accgacactc tccgcagcgc ctttctggaa cagggccagc cactcggcac cattcatcgg
     7861 cggcatattg agttcaacgc cactttgttc cggcaacggc gggagcgttt tactgtctgc
     7921 cgcccaaata gcacgatcga gcgtcagctt ttgaccaagc aaccagcggc tattgaaatg
     7981 gttatccgca ccagcctgtc cggttaattc aaagctgttg agattgccat caaccttaac
     8041 gtttaccgct agtggttcac ccgcaggttt ggctaacggt gaaggtaagt gactactcac
     8101 attattcaga tcgccgttca tctctacgtt ataggtcgcg ccagcatgat aaggcagatc
     8161 aatgcccact ttaccatccc acgccacgct gccactcaat gcttcgttca ccgcttcagg
     8221 cagaacgccg gttttcgccg gttgccagtt accgttgagg tttaccgcta cctggtaggc
     8281 ttttgcccct tctttggtgg aaaaatccac gttcaacggc tgattaaacc agcttgctgt
     8341 cagtggttca ctttgcagat cgccattgat aaagctgaat ttaccgctca aatttttcag
     8401 ggtgctgtcg agtggtttga taaacagact gttattacgc agcgtcactt cacctttcgc
     8461 ggttaccaac tcgccgttca gcgggatatc aagatgtaag cgagcattca catcgccgtc
     8521 gagctggagt tcttgcaggg tcgcacccag ggaatctttc agcggtgtct catcaaagta
     8581 agggcctacg gctttacccg gacctttaat gtcagcgtca atcagcagtt tttcttttga
     8641 gtagtcaggg atcactgcgg tgagattact cgcgcgcacg ccgcccagat taacgccatc
     8701 ggttttcatc cataaaccgt cgttaataaa gtccagttca atatcaaggt tagttaatgc
     8761 aggccagtcc ggctggaagg caaacttcgc gttgcgcagc ggcaccagca cttcaaactg
     8821 accttcgttg tgtttatagg ggaagagttg cggattgcca ccataaacca gcgtcgcgtt
     8881 atccgcttca ccgccctgaa tcgcgccact taagtaatca accaggtctt tacccatcaa
     8941 gttttccggg aaatagcgcc aggcttgtga accatcatcg gtactgatgc cagccagaat
     9001 acccagccag ggttcatcgt tagcaggttg caggtaacga aaaccgccgc gcgcatggac
     9061 ggctttggct ttaacgtcaa tattacgccc atccagctgg aaacctttgt cattgttcag
     9121 ccagcttata gttgcctggc cgtcggcgat ttccagtggc gcacggaata ccgtttcgta
     9181 aggcatcttt gcctgcttca tcgacgcggt aagcaaacca ttttcaacgc tgccggaaag
     9241 cgtcccggag aagtgttccg cacccggtaa taatttccat tgcttccagg ccagatcgct
     9301 ccacgatgcc tgaaaacggg tattgtctgc cgcctgaagc gggatatcca gcgccagagt
     9361 gttaatcttg ccgctcggtt gtgtggagcg ccaaacatca cccagtgcag gtgaaagttt
     9421 cgcggccagc gggcgtatgc cctccaggcc tgccagctcc agattactgg cgcgaatccg
     9481 gagttcgtca ctgcgtttat tgtctttgcc gccaacgtcc tgttccggta tccaggccag
     9541 cgtcaatgct ccgctcggcc agggtttacc gtccatcgtg atccgtgtat cgggaataga
     9601 gaactgccag cccggatttt cacgcgtaat atgcgcggtc agattatcca ccgacagcgt
     9661 atgcgtttcc ttctcgccca accagctggc accgccctgt ttcagccaga cgtcaccgcc
     9721 ggttacatcg cctttgtcga tcgtcatcca gccttcaagg gagaactgtg ccgtttccag
     9781 agcaatattg tcctgcatcc atttaccgag ccacggcttc aggtcgatgt catccgcctg
     9841 tagccagacg cgaccattgc ttaacaaccc ctcatcatcg cgcaaatcca tgcgcacctg
     9901 catcacgccg tgctgtccgg taaggctgga gaggcttacc aggccttccg cacggtgtcg
     9961 acgtggatcg ttcagccagg tgagttgtgg gatcgccagc tcggcgcgct gaccggatgg
    10021 cgtcagaaaa ctgacttcac tgtcgcggag atcgaaatgg tcaaattgac gaagaaacag
    10081 atcgctgatg tgactggctt ccagactgtc atcgctacca ccgctggtga taggagtgtt
    10141 ggtgcgaaag cgcagctgcc agaaagtgag gtcgcgaaac tgccagcgca tatgtaacag
    10201 gctctgccag acatccagcg ccagagtaac gcgtttaacc gaaaattcgc cgccatcttt
    10261 tagttctgca cggatgtcgt gtgcttcaag cgtcgggcca aaattctgcc agctggctga
    10321 gagctgactg gcttctaccg gcatgccagt cgcggattct attttgttga ggatttccgg
    10381 acgccaggcg tcaagatgcg gtaaagcaat acgcaggccg ctaaccagca gggctgcgat
    10441 cacaacgagc gcggctccag taagcagtaa aatccccggc aatcgcctca cccgtcactc
    10501 cttgtctgct aaaaatgtga ctcaaaaacc ctttgccgga tggcggccca gcatctgttt
    10561 acatcattac gacgtcaaac tgctcctggt tatagagcgg ttcaatttgt actttaacct
    10621 gtttaccaac gaaaatttcc acttccgcca gcgagtgtga ctcttcgcct ttcaaggctt
    10681 cagctactgc cggagaagca tagaccagga aacggtcgga gtcgtaagca tggtggacac
    10741 gaacaatctc gcgcatgatt tcatagcata ccgtttccac ggttttcacc gttccgcgac
    10801 cgtggcaggt tgggcattcg ttacacagta cgtgctcaat gctttcgcgg gtgcgtttac
    10861 gcgtcatctc caccagcccc agcgccgaaa aaccattaac actggttttc acccggtctt
    10921 tgctcaacgc ctgctccagc gagtgcagca cgcggcggcg gtgatcttca ttattcatat
    10981 cgatgaaatc aataatgata atcccaccca gattacgcaa ccgtaactgg cgagcgatag
    11041 cctgcgtcgc ttcaatattg gtattgaaaa tggtgtcgtc cagattgcga tgaccgacaa
    11101 acgctccggt attgatgtcc acggtggtca tcgcttcggt ctggtcgata atcagataac
    11161 caccggattt cagttctact ttgcgttcca gcgctcgctg gatttcgttt tcgacatcaa
    11221 agagatcgaa aatcggctgg cgtccggtgt aatgctccag cttgctggtc atctcgggaa
    11281 tgtactccga ggtaaactcc agcaatgctt cgtaagtcag gcgtgagtca acgcgaatgc
    11341 ggtccagttc ggcgtcggcg aaatcacgca gaacacgctg cgccagcgcc agttcgccgt
    11401 acagctgata acgggtctgc gggcgttttt tacgctccat aactttggtc cagacgcgtt
    11461 tcagataagc ggcatcggag gccagttcag cctcgccaac cccttccgct gcggtacgga
    11521 tgataaaccc gccctgctcg tcgcaatact ctgcgaccac ttttttcagg cgttcacgtt
    11581 ctgattcgct ttcaatacgt tgggaaacac caacgtgaga agcccctggc ataaagacca
    11641 gatagcgaga aggcagcgtg atatcggtgg tcaggcgcgc ccccttagtg ccaagcggat
    11701 ctttcaccac ctgcaccatc agatcttgcc cctgacgaac cagttccgag atgtcgcgca
    11761 ccgtgaattg cttttgttct tcacccgcca cacattcggt gtgcggcatg atatcggatg
    11821 catgaagaaa cgcggcttta tccagcccaa tatctacaaa agccgcctgc atacccggaa
    11881 gtacacgact tacacgaccc ttgtagatat tgcctactat tccgcgtcgc gcctcacgtt
    11941 caatatgaat ttcctgcaga ataccgccat caatatacgc cactcgcgtt tccgaaggcg
    12001 ttacgtttac taacaattca gccgtcatgt ttatcccttt tctcacgcag tgcgttaaaa
    12061 ttacttaata attcatacgt ttcaaccagc ggtaagccga ctacggcgtg atagctgcca
    12121 tttatcttcc tgacaaaaca gccacccagc ccctgaatac cgtatgcacc tgctttatct
    12181 aacggttcac cgctggcgac atagcccgcg atgtcttcgt ctgttaacgt tctgaaagtc
    12241 acatcggtga ccaccaggca atcgagaatg tgctggctgt cggccaacgc cactgccgtc
    12301 atcacctgat gggtctgacc cgataatttg cgcaacatct gcgccgcatg ctctgcgtcg
    12361 cgcggtttct ccagcacttc tccattcagg ataacgatag tatccgcacc cagcaccggg
    12421 agatcctgcg ccgtttgcgc gacacccgcc tgtgctttct cgcgcgccag acgcacaaca
    12481 tactgctgcg cgctctcctg cggctgacgc tgctcctcaa tgcccgtaac aatacgttca
    12541 aaggtcacgc caagttgcgc aagtaactcc tgacgacgcg gagaaccgga agctaaatac
    12601 agagaagtca tagaaacctt tattgcactg caaactgctg acggacttta cgcatcagca
    12661 agaaaatcca cggccagagc accccattga ctacactact ccagaacact tccggtctga
    12721 aagagacgtt aatcactaaa aactctgccc agaaaacaat aatatccacc accagcgaaa
    12781 gcaacatgac gaccagcgcc tgctgccata atgcgaggtt gcggaaaagc tggtatttca
    12841 gcgccaccag gtaagcaatg atgctcatcg ccaatacgcg tacgccaagc gtcgagccgc
    12901 tgatcagatc cagtatggca cccatcacaa aacctgtgcc cacatttacg cgatgaggca
    12961 aggccaggat ccaatacaac aagatgagta acacccagtt tggccggaaa acaatcaggt
    13021 tatccggcca gggcatgatt tgcagcaaca gcgcaatgag gaaagagagc cagattaccc
    13081 aacgtccctg gctacgatag ctcgccacta ttgccctccc ggcgcacgcg caggcggttg
    13141 agcaccactt tgcggcggcg tagccctttg tggagagcgg ttagcagcag gctgtgtcgg
    13201 cgcagcaggc gcagtagctg catttcctgt cgtcggttgc tgcggagtcg gctgagcgat
    13261 ccccgttgcc ggttcaggta actttggccc catcgcgtct ggcgaaggca atacctgcgg
    13321 catcatctgc atcagacgtt cattagcaac acgatgcacc tcttccggcg tcatcgggtt
    13381 agcgccgtta cgatccgcgc cccacagcag cagcagataa cgcaaacgtt gcagccctgc
    13441 tgtcggacgc gcctgaatca cagtataagc gcgctgggta tcgagtttta cggaagagac
    13501 aaccgcgacc ggatagcctt ccgggaaacg accgcccaga ccggaagtca ccagcacatc
    13561 accaacacga atatccgtat tcactggtag atgctcaagc tgcaaatcat ccgtacaacc
    13621 gttaccggct gcaattacgc ggatatcgtt gcgcagcacc tggattggca gcgcgtgggt
    13681 cgcatcacaa atcagcagca cgcgactggt cagtttagcg acggccacca cctgaccaac
    13741 aacacctttg tcgctgatga ccggctggcc ttcataaacg ccattaacgc tacctttatc
    13801 gataacaact tgatcgctat aaggatcgtt aaccgtggag ataacctgag tcaccatttt
    13861 ctgctcatcc tgacgcagcg gggaacccag cagctcgcgc agacgcgcgt tctcctgttt
    13921 gtattgtcca agcatcagca gttcactgtt tttcagcaac agttcctgac gtaacgcccg
    13981 gttttcaagt tctagttggt cacgcgaggc cagcgtctgc gatacgccat ccagcaattc
    14041 acgaggagca ttggaaacaa agtagaaagg actgacggcg gtatccatat aagtacggat
    14101 ttgactgaac gtccccaggc ggctgtcggc aataataatg ccgagcgcca ccagcaccgc
    14161 cagaataagg cgaatctgta gcgacgggcc acggctaaaa attggcttca taagttatgc
    14221 gtattctcgt atcagaccag gcagggtaaa cagacatttc ccctgcctgc atccgattac
    14281 tcttcgctga acaggtcgcc gccgtgcatg tcgatcattt ccagcgcttt gccgccaccg
    14341 cgcgccacac aggtcagcgg gtcttcagca acaacgactg gaatgccggt ttcttccatt
    14401 aacaaacggt caaggttacg cagcagtgcg ccaccaccgg tgagcaccat gccgcgctcg
    14461 gagatgtcgg aagccagttc cggcgggcac tgttccagtg caaccattac cgcgctcaca
    14521 ataccggtca gcggttcctg cagtgcttcg aggatttcgt tggagttcag ggtaaaaccg
    14581 cgtggaacac cttctgccag gttacggcca cgaacttcga tttcacggac ttcatcgccc
    14641 gggtaagccg aaccgatttc gtgcttgata cgttctgcgg tggcttcacc gatcagagaa
    14701 ccgtaattac gacgcacata gttgatgata gcttcgtcga aacgatcacc accaatgcgc
    14761 acggaagagg agtaaaccac gccgttcaag gagataacag caacttcagt ggtaccacca
    14821 ccgatatcaa ccaccataga accggtcgct tcagaaaccg gcaggccagc accaattgcg
    14881 gcagccatcg gttcttcaat caggaagact tcacgggcac cagcgccctg cgcggattca
    14941 cgaattgcgc ggcgttcaac ctgggtcgcg ccaaccggta cacaaaccag aacgcgcggg
    15001 cttggacgca taaagctgtt gctgtgcact tgtttgatga agtgctggag cattttttca
    15061 gtcacgaaga agtcggcgat aacgccgtct ttcattgggc gaatggcagc aatattgccc
    15121 ggcgtacggc ccagcatctg cttcgcgtca tgacctactg cagctacgct tttcggtgaa
    15181 ccggcacgat cctgacgaat ggccaccacg gaaggctcat tcaatacgat gccttgtcct
    15241 tttacataaa tgagggtatt cgcagtaccc aggtcaatgg acaagtcatt ggaaaacatg
    15301 ccacgaaatt ttttcaacat actaagggat aatcctgaaa gctggggcgg aaaagaaaat
    15361 ccgcttactt taccaaccac acgcagcagc gacaaggcgc aaaaatcatc tgctacggtg
    15421 aaaattagtg cagttcgttt cctttgttac aaatctctgc ctgagtccag aaaggcttaa
    15481 tgcatcagca gcattcctcg cctgtttgca accgcgtaag gtcattcatc tgcatatgtg
    15541 ctgcaacaat ctggcgagca gacaagcaca ctcccatgag acgcagcgcg cgttattcta
    15601 cgtgaaaaca gattaaacgg caggttaaac cgagtatctt tgtgaatatt ttttcacgtt
    15661 agtatcaagt ggctgtgagg acgcgaaaaa atccccttgc ccgcctgtaa caccgcgctg
    15721 aatcagggtc tgccactcgc ttcgcgaacg cacaccggtg gcgtaaactt gggtgctggt
    15781 cccggagcag gcttccacca ggctttgaac cagcagctgg ttctccgttc gcttctcaat
    15841 gtttctgacc agccccggat ggagcttgag taactcaaca ttaagttctt tgatccaact
    15901 ggtgcttacc agcgtcaaac cagcctggtt gacggctacc cgtaccccta aagcattcac
    15961 taaacgaata acaggttgta aacgactgat atgttgacct acatcggcct ctgcaagttc
    16021 aataattatg cgttttcgtt gtgatttttc acattgcatt aacgtatcgc gcagccaacg
    16081 ctgaaaacgc gggcgaatca gcgactcaac ggtaacctga atcgccagat tttcctctgg
    16141 ccagtaacgc aatagtggaa taagacggct gatttgcaga cggtcatact cttccgataa
    16201 gccaaactgc aagaccatcg gcatatactc cgccgagcta acctcttcat taccatcgaa
    16261 gatgcggcac atgagttcgc gatgatgaac ctgaccttcg cgagtaaccg ccggtttttg
    16321 ataaaggcgc gggccgccgc gactgagcat ttgctcgata agcgtacgcc agcgaacatt
    16381 accgcgtcct ttttcaggca acgagtcatc gtaaatagcc cagctattgc cgccctgcaa
    16441 tcccgcatta cgcgtggcag actctgcatg ttccattacc tgctcggtat cctgaccact
    16501 acgccaggcg cagataccaa tgtggatcat atcgtcgcga tcgagcattt tattgttcgg
    16561 caaggtatcg acggctttga ttaactgacc ggcgatgctc tctgcctctt ttaacgtccg
    16621 gtgcggtaac agcgcagcaa aatcactgcg gtggtaacgc gccagcagtg cgccagggta
    16681 gcgcatcata aatgtcgaca gcagattcgt cagagtgaag aactgttctt caacctggct
    16741 gtgcccccag gtatcgctca acatattgaa atccggcaga cgaatcatca tcacgatccc
    16801 gtgggtacct actttctcct gatcttccag taacgttgct aactgattat cgaaaaagag
    16861 tcggttattg aggccggttt tcacgtcctg ggcggcataa gagcggatca gcgtatcaag
    16921 acggctgtgt tgttcgcgtg cgttctgaat ttcacgaagc agcgtatcta gcgcactgct
    16981 ggttctgggc ggccattcat agatggttcc caacacatta gagccacgct caccgtttaa
    17041 gatacgagta gcccgggttt ccagcaattc ttgcccggca agttgccgtt gtaaccagcg
    17101 taccgccagg aagagcataa caatgataaa gccaatcgcc cccgtgagcg gcgcggtagt
    17161 cattaaagaa tggaaatagt tgcccatcgg atcctgataa accagacgca gcgacatccc
    17221 cggatgcttt atcaacggaa cactcagttc gcgaaacaga tcgctggagc caaccggacg
    17281 ataactacca tttctggcca gggtataaac ctgtttatca ccatggagca ggtctacacg
    17341 aacgatatct gccgacatca ttaattcggt aatttgtggc cttaatgtgc tgaagtcatt
    17401 cgacacaagg tgggtatcaa tcgccgtcgc caccgcctga acgcgatgac taaacttata
    17461 ctgaattgcg ttgtagaaac ttagcgaaca gcccagcaaa gtcacaaaaa ttgttaaccc
    17521 ggtgagcagc gtaacaaagg ccgaaaattt cgtcgttaat ctcatccttg tgttaactcc
    17581 gatagtgagg aagcgggcat actagcaaat cagatttatc tcgcaattta ttgcgcttca
    17641 tcggctttgc ttttccatta gcgagtatag tcttcagaaa ttattttcca atccatcatg
    17701 cacatgagga ccacttatgc aggcgttact tttagaacag caggacggca aaactctcgc
    17761 atcggtacag acacttgacg aaagtcgcct gccggagggc gatgtcacgg ttgatgttca
    17821 ctggtcgagc ctgaactata aagatgcgct ggcgattacc ggtaagggaa aaatcatccg
    17881 taattttccg atgattcctg ggatcgattt tgccggaact gtacgcacca gcgaagatcc
    17941 gcgttttcat gccggtcagg aggtgttact cactggctgg ggcgttggtg aaaaccactg
    18001 gggtgggctg gcggagcagg cgcgagtgaa aggtgactgg ctggttgcca tgccgcaagg
    18061 gctggacgcg cgtaaagcaa tgattatcgg tactgccggt tttaccgcca tgctgtgtgt
    18121 gatggcgctg gaagatgccg gtgttcgccc gcaggacggg gagattgtcg tgacgggtgc
    18181 cagtggtggc gtcggcagta ccgccgtggc gctgctgcat aagttgggtt atcaggtcgt
    18241 tgccgtttcc ggtcgcgaaa gtacccatga atacctgaaa agtttaggtg ccagtcgtat
    18301 tctccctcgt gatgagtttg ccgaatcccg tcctctggaa aaacaagtct gggctggggc
    18361 aattgacact gttggcgaca aagtgctggc aaaagtgctg gcgcaaatga attacggcgg
    18421 ctgcgtggcg gcctgtggtc tggcgggtgg ttttactctg ccaaccacgg tcatgccatt
    18481 tattctgcgt aatgtccgtt tgcaaggggt ggattcagta atgacgccac cagaacgccg
    18541 cgcacaagcc tggcagcgac tggtcgccga tttaccggaa tcattctata cccaggcggc
    18601 aaaagagata tctctgtcag aggcaccgaa ctttgccgag gccatcatta ataaccagat
    18661 ccagggtcgc acgctggtga aggttaacta accatttagc aaggaataat aagagaggga
    18721 actcattttg acatcattat tcaatcggtt aacgggaaaa gcggttagcc ggacagcttt
    18781 cgtcgaacac cttggtcagg aagttgtaca acatcatcca aactggaaag tcatgatttc
    18841 gactgaccac aaattgatgc gcattgatac tcctctaaac agccattatt gatatgcctc
    18901 cgtcgcctgt taggtttatg gtgctttgcc tgggcgacac tacgcttaac ctattactga
    18961 tttctggtcc catggggtga ataatctgat tttatttgac tacaaattaa ccactcgaac
    19021 ctatttaatg ctgagcattg tcagtcggtt gattttgcgt gctttagcat tcacatctat
    19081 ccagacgatg cagtgaaaat tgggtaatcc ccagcaaccg ctgcgtaatg tcgtctatct
    19141 tgtcgcgatc ctggcatccc tacattattt gtggtctgtg aagattatct cattgcagcc
    19201 cctcatcttc gcagggctgg ctttacagct tttcacctta cgttataaga agttccgtcg
    19261 atgatggcgc taatttcgtg aattgtgcgg cttgttgcaa attacacggt gttgaaggtt
    19321 atttacatgt tagctgttga ttatcttccc tgataagacc agtatttagc tgccaattgc
    19381 tacgaaatcg ttataatgtg cgacctcgtc ctccctgaca cagtttttgc gctgcggaaa
    19441 aggtgacatt ggcgcaacga aggtatattt tgttttttgc cggaggatag cagcagatcg
    19501 ctgcacaatg tccgtcaagt ctaacattga cactctgggg caaaatagac cggcgtcccg
    19561 gcctgctgga atttatcgct atgcatacag ctgtcggggc atacgcttta cagacggcgg
    19621 tgaaacgcct gtcacaatca cactaaacaa agagtacgga acccactcat ggatattcgt
    19681 aagattaaaa aactgatcga gctggttgaa gaatcaggca tctccgaact ggaaatctcc
    19741 gaaggcgaag agtcagtacg catcagccgt gcagctcctg ccgcaagttt ccctgtgatg
    19801 caacaagctt acgctgcacc aatgatgcag cagccagctc aatctaacgc agccgctccg
    19861 gcgaccgttc cttccatgga agcgccagca gcagcggaaa tcagtggtca catcgtacgt
    19921 tccccgatgg ttggtacttt ctaccgcacc ccaagcccgg acgcaaaagc gttcatcgaa
    19981 gtgggtcaga aagtcaacgt gggcgatacc ctgtgcatcg ttgaagccat gaaaatgatg
    20041 aaccagatcg aagcggacaa atccggtacc gtgaaagcaa ttctggtcga aagtggacaa
    20101 ccggtagaat ttgacgagcc gctggtcgtc atcgagtaac gaggcgaaca tgctggataa
    20161 aattgttatt gccaaccgcg gcgagattgc attgcgtatt cttcgtgcct gtaaagaact
    20221 gggcatcaag actgtcgctg tgcactccag cgcggatcgc gatctaaaac acgtattact
    20281 ggcagatgaa acggtctgta ttggccctgc tccgtcagta aaaagttatc tgaacatccc
    20341 ggcaatcata agcgccgctg aaatcaccgg cgcagtagca atccatccgg gttacggctt
    20401 cctctccgag aacgccaact ttgccgagca ggttgaacgc tccggcttta tcttcattgg
    20461 cccgaaagca gacactattc gcctgatggg tgacaaagtg tcggcaatcg cggcgatgaa
    20521 aaaagcgggc gttccttgcg taccaggttc tgacggcccg ctgggcgacg atatggataa
    20581 aaaccgtgcc attgctaaac gcataggtta tccggtgatt atcaaagcct ccggcggcgg
    20641 cggcggtcgt ggtatgcgtg tagtgcgcgg cgacgctgaa ctggcacaat ccatctccat
    20701 gacccgtgcg gaagcgaaag ccgcattcaa taacgatatg gtttacatgg agaaatacct
    20761 ggaaaatcct cgccacgtcg agattcaggt actggctgac ggtcagggca acgctatcta
    20821 tctggcggaa cgtgactgct ctatgcagcg tcgtcaccag aaagtggtcg aagaagcacc
    20881 agcaccggga attacaccgg aactgcgtcg ctacatcggc gaacgttgtg ctaaagcgtg
    20941 tgttgatatc ggctatcgcg gtgcaggtac tttcgagttc ctgttcgaaa acggcgagtt
    21001 ctatttcatc gaaatgaaca cccgtattca ggtagaacac ccggttacag aaatgatcac
    21061 cggcgttgac ctgatcaaag aacagctgcg tatcgctgcc ggtcaaccgc tgtcgatcaa
    21121 gcaagaagaa gttcacgttc gcggccatgc ggtggaatgt cgtatcaacg ccgaagatcc
    21181 gaacaccttc ctgccaagcc caggcaaaat cacccgtttc cacgcacctg gcggttttgg
    21241 agtgcgttgg gagtctcata tctacgcggg ctacaccgta ccgccgtact atgactcaat
    21301 gatcggtaag ctgatttgct acggtgaaaa ccgtgacgtg gcgattgctc gcatgaagaa
    21361 tgcgctgcag gagctgatca tcgacggtat caaaaccaac gttgatctgc agatccgcat
    21421 catgaatgac gagaacttcc agcatggtgg cactaacatc cactatctgg agaaaaaact
    21481 cggtcttcag gaaaaataag actgcaaaag cgtcaaaagg ccggattttc cggccttttt
    21541 tattactggg gatcgacaac ccccataagg tacaatcctc gctttcttca cccatcaggg
    21601 acaaaaaatg gacactcgtt ttgttcaggc ccataaagag gcgcgctggg cgctggggct
    21661 gacccttttg tatctggcag tttggttagt agccgcttac ttacctggcg ttgcccccgg
    21721 ttttaccggc tttccgcgct ggtttgagat ggcctgcatc ctgacgccgc tactgtttat
    21781 tggactgtgc tgggcgatgg tgaaatttat ctatcgcgat atcccactgg aggatgacga
    21841 tgcagcttga agtaattcta ccgctggtcg cctatctggt ggtggtgttc ggtatctcgg
    21901 tttatgcgat gcgtaaacgg agcaccggca ccttccttaa tgagtatttc ctcggcagcc
    21961 gctctatggg cggtattgtg ctggcgatga cgctcaccgc gacctatatc agtgccagtt
    22021 cgtttatcgg cgggccagga gctgcttata aatacgggct gggctgggta ttgctggcga
    22081 tgattcagct tcctgcagtc tggctttcac tcggtattct cggcaagaag tttgcgattc
    22141 ttgcgcgccg ctacaatgca gtgacgctga acgatatgct gtttgcccgc taccagagtc
    22201 gtcttctggt gtggctggcg agtttgagtt tgctggttgc gttcgttggt gcgatgaccg
    22261 tgcagtttat cggcggtgcg cgcctgctgg aaaccgcggc gggtattcct tatgaaaccg
    22321 ggctgctgat ttttggtatc agcattgcgt tatataccgc ctttggtggc tttcgcgcca
    22381 gcgtgctgaa cgacaccatg caagggcttg tgatgctgat tggcaccgtt gtgctgctta
    22441 ttggcgtggt acatgccgct ggcggcttaa gcaacgcagt acagaccttg caaaccatcg
    22501 atccgcaact ggttacgcca caaggcgctg acgatattct gtcgcctgcc tttatgacgt
    22561 cgttctgggt actggtgtgc tttggcgtga ttggcctgcc gcatactgcg gtgcgctgta
    22621 tctcttataa agacagcaaa gcagtacacc gggggatcat catcggtacg attgtagtcg
    22681 caatcctgat gttcggtatg cacctggccg gagcgttagg tcgtgcagtg atccccgatc
    22741 tcaccgtacc ggatctggtg atcccgacgt taatggtaaa agtgctgtca ccgtttgctg
    22801 ccggaatctt cctggctgca cctatggctg cgatcatgtc gacgatcaat gcccagttgc
    22861 tgcaaagttc cgctacgatc attaaagatc tctatctgaa tatccgtccg gatcaaatgc
    22921 aaaacgagac gcgtctgaag cggatgtcgg cggtaattac gttagttctc ggcgcgttgc
    22981 tgctgcttgc cgcctggaag ccgccagaaa tgatcatctg gctgaatttg ctggccttcg
    23041 gtgggctgga agccgttttc ctgtggccgc tggtgctggg tctttactgg gaacgcgcca
    23101 acgccaaagg cgcgctaagt gcgatgatcg ttggcggcgt gctgtatgcc gtactcgcga
    23161 cgctgaatat tcagtacctg ggcttccacc ctattgtgcc ctcgttacta ctaagtttgc
    23221 tggctttcct ggtcggaaac cgtttcggta catccgtccc gcaagctacc gttttgacta
    23281 ctgataaata aagagttttg ccatgccttg gatccaactg aaactgaaca ccaccggcgc
    23341 gaacgcggaa gatcttagcg atgcgctgat ggaagcgggt gccgtttcta tcacttttca
    23401 ggatacccac gatacgccag tatttgagcc gctgccgggc gaaacgcgcc tgtggggcga
    23461 caccgatgtg attggtctgt tcgacgctga aaccgatatg aacgacgtgg tggcgattct
    23521 ggaaaaccat ccgctgctcg gcgcaggctt cgcgcataaa atcgaacaac tagaagataa
    23581 agactgggag cgcgaatgga tggataattt ccacccgatg cgctttggtg aacgactgtg
    23641 gatctgccct agctggcgtg atgtgccgga cgaaaacgcc gtcaacgtga tgttagatcc
    23701 agggctggcg tttggtacgg gtacccatcc aaccacctct ctgtgcctgc aatggctcga
    23761 cagcctcgat ttaaccggta aaacagtcat cgactttggc tgtggttccg gcattctggc
    23821 gatcgcggcg ctgaaactgg gtgcagcaaa agccattggt attgatatcg atccgcaggc
    23881 gattcaggcc agccgcgaca acgccgaacg taatggcgtt tctgaccgtc tggaactcta
    23941 cttaccgaaa gatcagccag aagaaatgaa agccgacgtg gtggtcgcta acatccttgc
    24001 aggcccatta cgtgaactgg caccgttaat cagcgtcctg ccggtttcag gcggtttgct
    24061 gggcctttcc ggtattctgg caagccaggc agagagcgtt tgtgaagctt atgccgatag
    24121 cttcgcactg gacccggtcg tggaaaaaga agagtggtgc cgtattaccg gtcgtaagaa
    24181 ttaaccttcg catcgccgta gggtgacgcg gggcgaagtg cgagcaagct cacaaaaggc
    24241 acgtaaattt gccggttatt tacgcaaatt tgcgtgccaa aattttcatt cataaagaaa
    24301 aattgagaac ttactcaaat ttctttgagt gtaaatttta gtcactattt tctaatatga
    24361 tgatttttat gagtaattat cgcaccacgc tcattttaaa tgcaattctt tgatccatct
    24421 cagaggattg gtcaaagttt ggcctttcat ctcgtgcaaa aaatgcgtaa tatacgccgc
    24481 cttgcagtca cagtatggtc atttcttaac tcatgcgcat cggacaatat cagctcagaa
    24541 atcgcctgat cgcagcgccc atggctggca ttacagacag accttttcgg acgttgtgct
    24601 acgagatggg agccggattg acagtatccg agatgatgtc ttctaaccca caggtttggg
    24661 aaagcgacaa atctcgttta cggatggtgc acattgatga acccggtatt cgcaccgtgc
    24721 aaattgctgg tagcgatccg aaagaaatgg cagatgcagc acgtattaac gtggaaagcg
    24781 gtgcccagat tattgatatc aatatgggtt gcccggctaa aaaagtgaat cgcaagctcg
    24841 caggttcagc cctcttgcag tacccggatg tcgttaaatc gatccttacc gaggtcgtca
    24901 atgcagtgga cgttcctgtt accctgaaga ttcgcaccgg ctgggcaccg gaacaccgta
    24961 actgcgaaga gattgcccaa ctggctgaag actgtggcat tcaggctctg accattcatg
    25021 gccgtacacg cgcctgtttg ttcaatggag aagctgagta cgacagtatt cgggcagtta
    25081 agcagaaagt ttccattccg gttatcgcga atggcgacat tactgacccg cttaaagcca
    25141 gagctgtgct cgactataca ggggcggatg ccctgatgat aggccgcgca gctcagggaa
    25201 gaccctggat ctttcgggaa atccagcatt atctggacac tggggagttg ctgcccccgc
    25261 tgcctttggc agaggttaag cgcttgcttt gcgcgcacgt tcgggaactg catgactttt
    25321 atggtccggc aaaagggtac cgaattgcac gtaaacacgt ttcctggtat ctccaggaac
    25381 acgctccaaa tgaccagttt cggcgcacat tcaacgccat tgaggatgcc agcgaacagc
    25441 tggaggcgtt ggaggcatac ttcgaaaatt ttgcgtaaac agaaataaag agctgacaga
    25501 actatgttcg aacaacgcgt aaattctgac gtactgaccg tttctaccgt taactctcag
    25561 gatcaggtaa cccaaaaacc cctgcgtgac tcggttaaac aggcactgaa gaactatttt
    25621 gctcaactga atggtcagga tgtgaatgac ctctatgagc tggtactggc tgaagtagaa
    25681 cagcccctgt tggacatggt gatgcaatac acccgtggta accagacccg tgctgcgctg
    25741 atgatgggca tcaaccgtgg tacgctgcgt aaaaaattga aaaaatacgg catgaactaa
    25801 ttcaggttag ctaaatgctt gattaaaaag gcgctactcg gcatggggaa gcgccttttt
    25861 tataggtgtc acaaagggag tgaccatgag aacaggatgt gaaccgaccc ggtttggtaa
    25921 tgaagctaag accattattc acggtgatgc gcttgccgaa cttaaaaaga tccccgccga
    25981 aagtgtcgat ctgatctttg ccgacccacc atataacatc ggtaaaaatt ttgatggtct
    26041 gatcgaagcc tggaaagaag atctgtttat cgactggctg tttgaagtga ttgcagagtg
    26101 ccaccgcgtt ctgaaaaagc agggcagcat gtacatcatg aacagcacgg aaaacatgcc
    26161 ctttatcgat ctccagtgcc gcaagctttt taccatcaaa agtcgcatcg tctggtcata
    26221 tgacagttct ggagtacagg cgaaaaaaca ctacggctcc atgtacgaac ccatcctgat
    26281 gatggtgaaa gacgcaaaga actacacatt caacggtgat gctattctgg tagaagccaa
    26341 aaccggatcg cagcgcgcgt tgatcgatta tcgcaaaaat cctccacagc catacaatca
    26401 tcaaaaagta ccgggtaacg tctgggattt tccgcgcgtg cgttatttga tggatgaata
    26461 tgagaaccac ccgacgcaaa aaccggaagc cttactgaaa cgcattattc tcgcctcttc
    26521 caacccaggc gatatcgttc tcgacccgtt tgccggtagc tttactaccg gtgccgtagc
    26581 cgtctccagc gggcgaaaat tcattggtat tgagatcaac agcgagtaca tcaaaatggg
    26641 gcttcgacgg ctggatgtcg cgtcgcatta ctctgcggaa gaactggcga aagtgaaaaa
    26701 aagaaagacc ggcaacctgt caaaacgaag ccggttaagc gaagttgacc ccgatctcat
    26761 tgcaaagtaa aggaagtgta agcctggttt tcagattatt catttcgtgt atatttcctg
    26821 ccagacttgg ttaaacatac acaggctcag gtaatgattc gcaagtattg gtggctcgtc
    26881 gttttcgctg tcttcgtttt tctgtttgat actttactga tgcagtggat tgaactgctg
    26941 gcaacagaaa cagacaaatg ccgcaatatg aactcagtta atccactaaa actggttaac
    27001 tgtgacgaac tgaattttca ggacagaata tgaatttaca tgacacttaa ttcattcgtt
    27061 tgatgaatta atttcgttat gttttcatct ggcatgaaca ttcttaatac gttatcgacc
    27121 agagcggggg cttgtttata aagatcataa cccgccatat tcattaacca gttttgaaca
    27181 attccgctga aggcaccatt aataataatc atcacaacat ctaaatcgag gttatttgct
    27241 acacaacctt gttgctgaca cgcctgcaat acttcgcgga gagtctgcgg attgaagccc
    27301 atcttttcgc gtatcactcc ctcggccagc atctcatcat taaattcaca tttgtgatat
    27361 aagattttca gcaacgcctg ctggcgggga attttggcaa tatattgcaa gccgacaatc
    27421 aatttttcac gcaattgttg aaacgggtca tgctctaatc cagccgtcaa gtgttcctgg
    27481 attaactccc gcaatgaagg ctgttgcaac cacatctcat taaacagttg agtcttgttt
    27541 tcgaagtgcc agtagatagc gccacgcgta acgttagcag cgtcggcaat gtcgttgagt
    27601 gtcgtcttgc ttacgccatg ctgcgcaaac tgggcgatgg cagtttcaat cagttcttgc
    27661 cgggtcttca gagcttcggc tttggttctt tttgccatga ttaattattc aggaaataaa
    27721 tatattcgac acagagtgag aaaatagcga aggttaatct atcacctaat g
//
LOCUS       DAZFYC010000064        27328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFYC010000064 DAZFYC010000000
VERSION     DAZFYC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.064"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHKW5_002919"
     CDS             complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHKW5_002919"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO7512275.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHKW5_002920"
     CDS             complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHKW5_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO7512276.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHKW5_002921"
     CDS             complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHKW5_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO7512277.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHKW5_002922"
     CDS             complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHKW5_002922"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO7512278.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHKW5_002923"
     CDS             complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHKW5_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO7512279.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHKW5_002924"
     CDS             complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHKW5_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO7512280.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHKW5_002925"
     CDS             complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHKW5_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO7512281.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHKW5_002926"
     CDS             complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHKW5_002926"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7512282.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHKW5_002927"
     CDS             complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHKW5_002927"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO7512283.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHKW5_002928"
     CDS             complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHKW5_002928"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO7512284.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHKW5_002929"
     CDS             complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHKW5_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO7512285.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHKW5_002930"
     CDS             complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHKW5_002930"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO7512286.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHKW5_002931"
     CDS             complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHKW5_002931"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO7512287.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHKW5_002932"
     CDS             complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHKW5_002932"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO7512288.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHKW5_002933"
     CDS             complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHKW5_002933"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO7512289.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHKW5_002934"
     CDS             complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHKW5_002934"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO7512290.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHKW5_002935"
     CDS             complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHKW5_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO7512291.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHKW5_002936"
     CDS             complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHKW5_002936"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO7512292.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHKW5_002937"
     CDS             complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHKW5_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO7512293.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHKW5_002938"
     CDS             complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHKW5_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO7512294.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23548..23634)
                     /locus_tag="ACHKW5_002939"
     CDS             complement(23548..23634)
                     /locus_tag="ACHKW5_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO7512295.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHKW5_002940"
     CDS             complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHKW5_002940"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7512296.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHKW5_002941"
     CDS             complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHKW5_002941"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO7512297.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25983..26027
                     /locus_tag="ACHKW5_002942"
     CDS             25983..26027
                     /locus_tag="ACHKW5_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512298.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHKW5_002943"
     CDS             complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHKW5_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO7512299.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6534 a         7787 c         6555 g         6452 t
ORIGIN      
        1 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac
       61 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
      121 aagaccgact agtgacatcc actcaccggg ttgcccttct ttaccccacg gctccccttc
      181 ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
      241 tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa gttcacgcac
      301 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
      361 cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct cgttgcgaat
      421 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
      481 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
      541 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc accggtttgc
      601 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc
      661 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
      721 cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc cagcatcgct
      781 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
      841 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
      901 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
      961 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
     1021 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
     1081 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
     1141 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
     1201 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
     1261 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
     1321 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
     1381 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
     1441 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
     1501 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
     1561 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
     1621 tctacggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc gagcacaata
     1681 tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata acctgcctga
     1741 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg cttaataccg
     1801 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
     1861 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
     1921 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
     1981 acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt atcagcagta
     2041 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
     2101 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
     2161 ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
     2221 tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc
     2281 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
     2341 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
     2401 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
     2461 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
     2521 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
     2581 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
     2641 gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
     2701 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accgacagtc
     2761 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
     2821 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
     2881 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
     2941 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
     3001 cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgt
     3061 gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc catctccggt
     3121 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
     3181 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
     3241 gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
     3301 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
     3361 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
     3421 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
     3481 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg
     3541 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg cttccagcaa
     3601 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
     3661 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat
     3721 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
     3781 tccactcacg ttattgccat cccgatcata ttcaaacgta tttagcgcac aaatcttatc
     3841 attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag tgcttaagaa
     3901 aacagcgttc gcaccgtttt ttatagctct tataaaattt aaaaaaaaac gactggttca
     3961 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
     4021 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
     4081 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
     4141 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
     4201 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
     4261 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
     4321 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
     4381 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
     4441 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
     4501 tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg
     4561 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
     4621 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
     4681 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
     4741 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
     4801 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
     4861 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
     4921 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaactga agtgtactat
     4981 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
     5041 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
     5101 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
     5161 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
     5221 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
     5281 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
     5341 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
     5401 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
     5461 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
     5521 tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
     5581 cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat cgttcgctgc
     5641 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
     5701 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
     5761 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
     5821 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
     5881 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
     5941 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
     6001 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
     6061 atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt caacagcatt
     6121 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
     6181 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
     6241 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
     6301 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
     6361 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
     6421 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
     6481 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
     6541 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
     6601 cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
     6661 cggacgacca cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
     6721 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
     6781 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
     6841 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
     6901 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
     6961 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
     7021 gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc ctaccggatt
     7081 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
     7141 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
     7201 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
     7261 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
     7321 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
     7381 acgtgacggg cagcttccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
     7441 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
     7501 cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc
     7561 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
     7621 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
     7681 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
     7741 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
     7801 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
     7861 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
     7921 gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
     7981 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
     8041 tctatttgcg tctggatgat gttgacatct tgggtcataa aggtacccgg ctcacccaat
     8101 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
     8161 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
     8221 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
     8281 ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata
     8341 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
     8401 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
     8461 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
     8521 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
     8581 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
     8641 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
     8701 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
     8761 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
     8821 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
     8881 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
     8941 ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
     9001 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
     9061 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
     9121 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
     9181 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
     9241 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
     9301 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
     9361 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
     9421 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
     9481 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
     9541 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
     9601 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
     9661 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
     9721 ccagcgggaa ttcaccgagg aaatcaaaac ggcgcccagt cccctggaag ctctccagcg
     9781 cacgtaaaat agcctcgtca tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
     9841 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
     9901 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
     9961 cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc
    10021 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa
    10081 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    10141 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    10201 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    10261 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    10321 ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    10381 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    10441 cgacaatttc cgggttatcg gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    10501 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg
    10561 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    10621 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    10681 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    10741 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    10801 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    10861 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    10921 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    10981 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    11041 ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    11101 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccgcttcggc
    11161 atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga tagtgactga
    11221 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    11281 accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    11341 cggcagcgcc agtacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    11401 gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat tggttaagcc
    11461 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    11521 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    11581 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    11641 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    11701 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    11761 cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    11821 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    11881 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    11941 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    12001 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    12061 agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga catcgcgcga
    12121 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc cagttcttcg
    12181 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    12241 gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    12301 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    12361 atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat accgataatg
    12421 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    12481 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    12541 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    12601 agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    12661 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagcacg
    12721 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    12781 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    12841 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    12901 gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg
    12961 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ttttagcgcc
    13021 gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    13081 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    13141 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    13201 gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt tctgccactt
    13261 ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt
    13321 cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg ccgcccagca
    13381 acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta ctgccgacgt
    13441 tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc acttcaaagc
    13501 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag
    13561 ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc ccggaaagtt
    13621 tcatctcttt cacgttcagc actttctcgc ctttcactcg cagccaggtt tctccctgct
    13681 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatcagcac
    13741 cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc gcattttcgt
    13801 aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa cggatagcgg tccatatgat
    13861 cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct
    13921 ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc atcaacgctg
    13981 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    14041 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    14101 cttcgcggca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    14161 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    14221 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    14281 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    14341 ccacgcagga aagcccagtg aggcccaggc cgataatgac gacattttta ccctgataat
    14401 cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca tcagcgaaat
    14461 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    14521 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    14581 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    14641 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    14701 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    14761 cgtgcagaca atgaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    14821 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    14881 cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    14941 aatgacgaag taagccagca gaatgtagaa caaccccagc tgcggcatca catctttaaa
    15001 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc
    15061 gatacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca accctttggt
    15121 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    15181 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gtacggagat
    15241 cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    15301 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    15361 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    15421 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    15481 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta
    15541 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    15601 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    15661 tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    15721 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    15781 acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga accgacattg
    15841 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    15901 cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    15961 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    16021 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc
    16081 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    16141 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    16201 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    16261 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc agcttccggg
    16321 cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag ttcaataact
    16381 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    16441 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    16501 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    16561 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    16621 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    16681 tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg caacaaacag gcagcccggc
    16741 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    16801 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    16861 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    16921 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    16981 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    17041 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    17101 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    17161 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    17221 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    17281 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttta
    17341 acagatcagc cagcgggtag ccaagtgcca gcagtgtcgc cagcgcgagc agcaggttgc
    17401 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa cttgaactaa
    17461 agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt
    17521 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    17581 gccagcggcg gcccacttca tcgtcggcgt taacaatcgc ctgaccacaa tgatgttcag
    17641 aataaagcag ccatttcgcg gcttcgtagt gttccatatc accatgataa tcaaggtgat
    17701 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgctgta
    17761 ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgatcc accagccccg
    17821 ccagctcatg ctgaacatcg actgccgaac cggtggtatt ttctgtcggg atcactttcc
    17881 ccagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    17941 actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    18001 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    18061 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    18121 cacctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc
    18181 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    18241 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    18301 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    18361 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    18421 atgatggcac caaagaccgg cgctgaaacg gcgccgccgt agtatttacc cgcctgcgga
    18481 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    18541 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    18601 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    18661 acgctttcca tcatatgcac cacagtgcgg acaatggatt ccgggaagac acgttcaccg
    18721 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    18781 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    18841 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    18901 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    18961 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    19021 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    19081 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt
    19141 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    19201 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    19261 gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    19321 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    19381 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    19441 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    19501 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    19561 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    19621 agttttttga tgtagtccgc catgtcaggg ttcacctgac gcgccagata aataaagcgc
    19681 cctttcgggt tggcgttaat gcgggctgaa agctgatcca gcggaatatt gagcgcgtta
    19741 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    19801 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    19861 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    19921 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    19981 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    20041 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    20101 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    20161 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    20221 cattcaatat ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    20281 cgggtatggt gcgccgtggt taccacagtc accgccgtca aaataatgca aatgaacagg
    20341 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    20401 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    20461 atgcattcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    20521 cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt
    20581 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    20641 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    20701 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    20761 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    20821 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    20881 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    20941 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    21001 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    21061 gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    21121 tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    21181 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    21241 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    21301 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    21361 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    21421 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    21481 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    21541 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    21601 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    21661 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa
    21721 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    21781 agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    21841 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    21901 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    21961 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    22021 cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    22081 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    22141 ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    22201 agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca tctctgcatg
    22261 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    22321 tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga atagtcttat
    22381 ttactttaag gatattgatg tccattcccg tactctacgc gccagaggga aattcacctg
    22441 gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca ccgcggcgat agagattacg
    22501 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat
    22561 ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgccagca ccgggcactg
    22621 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    22681 accatcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    22741 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc
    22801 ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    22861 acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    22921 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    22981 ggcaccaacc acgctggtga aatgttcacg atcgagggcg cggtccagcg cgacaatcgg
    23041 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac
    23101 aataatggca tcaacctgac gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg
    23161 ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgctcaag
    23221 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    23281 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    23341 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    23401 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    23461 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    23521 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    23581 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
    23641 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    23701 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    23761 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
    23821 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    23881 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
    23941 ttgtgtaatt gcttttcgat ctgctcaagc actttttcat cgcccacggt ctggatggtc
    24001 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    24061 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    24121 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    24181 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    24241 acaccaggcg attattgcac acctgttcca gcgcctcgct aagtttgctt tccagctcat
    24301 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
    24361 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
    24421 tcaccatccc cagatagcgg ttattgagat tcaccaccag taccggcaac tcgtattgca
    24481 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
    24541 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    24601 tgccgaggcc accggaattg atccagcgac gcggtttgtc gaatggataa taaagtgcag
    24661 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc
    24721 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
    24781 actgacgagc gcgccactgt tcaatttgct gccaccagtc gcggatctca tccagtggtt
    24841 gatgggcgga ttcttgcgac aagagttcaa gcatttgttc gaggacctgg cgagcatccc
    24901 ccacaatcgg gatatccgca gtcacggttt tagaaatgga agtaggatca atatcgatat
    24961 gcagaacagt ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc
    25021 gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
    25081 cgtgcattcc cagcatgccc agcgcctgac gatgcgttgc cggaaacgcc cccagcccca
    25141 tcaatgagga aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
    25201 agcccgccat gattgcccca ccgcctacgt agacaaccgg tttttttgcc gctaccagcg
    25261 tttgcagagc gcgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca
    25321 tactgaccga ctcaggccag acatagggta atttgttcgc cggattaaga atatctttcg
    25381 gtaaatcaac gaccactggt cctgggcgac cacttgccgc cagccagaaa gcctttttca
    25441 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
    25501 aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
    25561 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
    25621 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg
    25681 ccagaccatc ggccatatgc actgccgcct gctcatgacg aactaaaaca tgatcgatac
    25741 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
    25801 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    25861 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    25921 tgcttaagca attggcagca agagacataa acgcagggta ataagaataa acaggaggaa
    25981 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgataa taaatcggct
    26041 gaatcccaca acttacagca atttctctat ttttttaatt aatcctaaaa cagagagccc
    26101 acacattcag aataaaattc tacattcaat aaaaaataca gaataaacca gacattcatg
    26161 cctgacctat tctgcattca gttagcgttt gcaaattgag actaattgct cttccatcca
    26221 ctgatggcct ttatcgcgcc cggcagcttc atgccaggag agataacagg ttctgctatt
    26281 ttgttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag
    26341 ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat
    26401 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact
    26461 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
    26521 cagtaacggg cccttaattg ttggatgatt tttgctggct accagcacca tttcatcttt
    26581 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
    26641 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
    26701 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
    26761 taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga
    26821 accaggcaat tcattttgta ctagttgcaa tgcctgacga actgagccaa aaagttgaaa
    26881 tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat
    26941 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc
    27001 ggcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
    27061 gaccatgcgt agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc
    27121 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
    27181 taatcctgaa gaatatgatt tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa
    27241 ttcggaaaac ataaagatac gggttctgat aaaaacagaa tccattgccg tggaatgagt
    27301 catttaccac agcataataa tccataat
//
LOCUS       DAZFYC010000065        27241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFYC010000065 DAZFYC010000000
VERSION     DAZFYC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.065"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..843
                     /locus_tag="ACHKW5_002944"
     CDS             <1..843
                     /locus_tag="ACHKW5_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO7512300.1"
                     /translation="RYSAVDLNSSKYGYYEKVGGGFYRYIESFNPISSETPNKIVYKG
                     ESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQ
                     LREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDS
                     DIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLS
                     VADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMV
                     NASK"
     gene            complement(1220..2581)
                     /gene="espK"
                     /locus_tag="ACHKW5_002945"
     CDS             complement(1220..2581)
                     /gene="espK"
                     /locus_tag="ACHKW5_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO7512301.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(2944..3807)
                     /gene="potB"
                     /locus_tag="ACHKW5_002946"
     CDS             complement(2944..3807)
                     /gene="potB"
                     /locus_tag="ACHKW5_002946"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO7512302.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
     gene            complement(3791..4927)
                     /gene="potA"
                     /locus_tag="ACHKW5_002947"
     CDS             complement(3791..4927)
                     /gene="potA"
                     /locus_tag="ACHKW5_002947"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO7512303.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5177..6406
                     /gene="pepT"
                     /locus_tag="ACHKW5_002948"
     CDS             5177..6406
                     /gene="pepT"
                     /locus_tag="ACHKW5_002948"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO7512304.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCNIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(6552..7673)
                     /gene="roxA"
                     /locus_tag="ACHKW5_002949"
                     /gene_synonym="ycfD"
     CDS             complement(6552..7673)
                     /gene="roxA"
                     /locus_tag="ACHKW5_002949"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO7512305.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            7922..9151
                     /locus_tag="ACHKW5_002950"
     CDS             7922..9151
                     /locus_tag="ACHKW5_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7512306.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFITQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            9515..9703
                     /locus_tag="ACHKW5_002951"
     CDS             9515..9703
                     /locus_tag="ACHKW5_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7512307.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            9761..10315
                     /locus_tag="ACHKW5_002952"
                     /pseudo
     CDS             9761..10315
                     /locus_tag="ACHKW5_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            10508..10705
                     /locus_tag="ACHKW5_002953"
     CDS             10508..10705
                     /locus_tag="ACHKW5_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO7512308.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVRHD"
     gene            10698..10910
                     /locus_tag="ACHKW5_002954"
     CDS             10698..10910
                     /locus_tag="ACHKW5_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512309.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            10900..11364
                     /locus_tag="ACHKW5_002955"
     CDS             10900..11364
                     /locus_tag="ACHKW5_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512310.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQFSADTALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            11357..11590
                     /locus_tag="ACHKW5_002956"
     CDS             11357..11590
                     /locus_tag="ACHKW5_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512311.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            11596..11895
                     /locus_tag="ACHKW5_002957"
     CDS             11596..11895
                     /locus_tag="ACHKW5_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7512312.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMVNGFRGKK"
     gene            11892..13292
                     /locus_tag="ACHKW5_002958"
     CDS             11892..13292
                     /locus_tag="ACHKW5_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO7512313.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            13493..13744
                     /locus_tag="ACHKW5_002959"
     CDS             13493..13744
                     /locus_tag="ACHKW5_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512314.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            13741..14151
                     /locus_tag="ACHKW5_002960"
     CDS             13741..14151
                     /locus_tag="ACHKW5_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7512315.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            14162..14434
                     /locus_tag="ACHKW5_002961"
     CDS             14162..14434
                     /locus_tag="ACHKW5_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7512316.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            14391..14519
                     /locus_tag="ACHKW5_002962"
     CDS             14391..14519
                     /locus_tag="ACHKW5_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO7512317.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            14561..14785
                     /locus_tag="ACHKW5_002963"
     CDS             14561..14785
                     /locus_tag="ACHKW5_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512318.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            15037..15243
                     /locus_tag="ACHKW5_002964"
     CDS             15037..15243
                     /locus_tag="ACHKW5_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512319.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQTHGGE"
     gene            15243..16298
                     /locus_tag="ACHKW5_002965"
     CDS             15243..16298
                     /locus_tag="ACHKW5_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7512320.1"
                     /translation="MAGKATALNTNQLFAYLNRGDIAEFKFSPLFTTLFFPNVATFNT
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNLIEGEGIERYE
                     IDWKIPESNIIEQADGKKWSELDKETHDPIYDIELYADQGNCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDIALVVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            16311..16646
                     /locus_tag="ACHKW5_002966"
     CDS             16311..16646
                     /locus_tag="ACHKW5_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7512321.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLAENGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESADTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            16659..17072
                     /locus_tag="ACHKW5_002967"
     CDS             16659..17072
                     /locus_tag="ACHKW5_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO7512322.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNSLEEKD
                     KPRADAIMALHESDDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            17278..17820
                     /locus_tag="ACHKW5_002968"
     CDS             17278..17820
                     /locus_tag="ACHKW5_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7512323.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            18077..18358
                     /locus_tag="ACHKW5_002969"
     CDS             18077..18358
                     /locus_tag="ACHKW5_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512324.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(18960..20420)
                     /gene="phoQ"
                     /locus_tag="ACHKW5_002970"
     CDS             complement(18960..20420)
                     /gene="phoQ"
                     /locus_tag="ACHKW5_002970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO7512325.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20420..21091)
                     /gene="phoP"
                     /locus_tag="ACHKW5_002971"
     CDS             complement(20420..21091)
                     /gene="phoP"
                     /locus_tag="ACHKW5_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO7512326.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21259..22629)
                     /gene="purB"
                     /locus_tag="ACHKW5_002972"
     CDS             complement(21259..22629)
                     /gene="purB"
                     /locus_tag="ACHKW5_002972"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO7512327.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(22633..23274)
                     /gene="hflD"
                     /locus_tag="ACHKW5_002973"
     CDS             complement(22633..23274)
                     /gene="hflD"
                     /locus_tag="ACHKW5_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO7512328.1"
                     /translation="MAKNYYDITLALAGICQSARLVQRLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23310..24416)
                     /gene="mnmA"
                     /locus_tag="ACHKW5_002974"
                     /gene_synonym="trmU"
     CDS             complement(23310..24416)
                     /gene="mnmA"
                     /locus_tag="ACHKW5_002974"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO7512329.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGGDYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(24470..24931)
                     /gene="nudJ"
                     /locus_tag="ACHKW5_002975"
     CDS             complement(24470..24931)
                     /gene="nudJ"
                     /locus_tag="ACHKW5_002975"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO7512330.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(24941..25594)
                     /gene="rluE"
                     /locus_tag="ACHKW5_002976"
     CDS             complement(24941..25594)
                     /gene="rluE"
                     /locus_tag="ACHKW5_002976"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO7512331.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            25766..27016
                     /gene="icd"
                     /locus_tag="ACHKW5_002977"
     CDS             25766..27016
                     /gene="icd"
                     /locus_tag="ACHKW5_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO7512332.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(27119..>27241)
                     /locus_tag="ACHKW5_002978"
     CDS             complement(27119..>27241)
                     /locus_tag="ACHKW5_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO7512333.1"
                     /translation="NKDLWSLLKSSQECPPGERKITNKCLHNSCIIKICPYGLK"
BASE COUNT         7234 a         6369 c         6913 g         6725 t
ORIGIN      
        1 agatatagtg cagtagatct taactcttct aagtatgggt attatgagaa agttggcggt
       61 ggtttttata gatatataga atcctttaac cccatatctt cagagacacc aaataaaata
      121 gtctacaagg gggaatcagt agatttaact aaggagccaa attcggaatt atattcaggt
      181 aggtattcta taaataacaa acaggttaat gtttatttct ttcgtgacgc tgatggtaca
      241 ttttataaat cagaaggtct tcatggtggg ggagttatta gatacataga taaaccgtat
      301 tctcagttaa gagaaggaga tattgggtat gatgaggatt tgttggatat atacgatgat
      361 tctccggtgc ttgaagacac gttgcctgct ttatcttctg aaatagtacc aactccagaa
      421 catagtatta aacaaattta ttcgaaaatt aaggaggggc acatagaact gtccgattca
      481 gacatcatat tgtgtcgcgg cacaaccggt attcaagctg aaaatatcgt tgaatataaa
      541 actgctggag ggcttcctga ttcaaatcca aatgtaaaag caccagatga atatatggca
      601 caacagcagg tacgtattgg aagaatattg cctgaataca catcggatct tagcgttgct
      661 gatcggttta gtcgtgagca ttatctaata gttgttaaag taaaggcaaa atatatcaca
      721 cgaggaagtg ttacagagag tggttgggtt atagataaga ccgcacctgt tgaaccactt
      781 gcgataattg atagaacttt tggtatgaag gaaaatatct caatggtaaa tgcatcgaaa
      841 tagttttttt acaatctatg tcctgcctcc tctggtaaaa acgatgctac atctttgaga
      901 tgttgcacgg cagtacggtg ttgaccgata tatagtgaag tacacatcgg tcaacgaatt
      961 accattgtca gcaatatcat cctgataaac tcagtactcg tgagccgctt gatgacgggc
     1021 ggaatagccc aaaaggtaaa cgatccgcat ggcagtccgt cggaaagctg ctgcttcata
     1081 cgaccgctta aaccgtcagt tagtgtcagt atcgctgaag atcagcttct tttgctgatt
     1141 tactctgttt ttacctgccc tgatgaaata ctctttaatc ataacattga ttatatcgaa
     1201 aatatatttt ttgctatcat taagaatatt tatatgtgga accggagtat tttggacggt
     1261 agtctttcca ctctggtgta tctaatattc tttctgctgg tatctctttg ccatgcagat
     1321 aaaattttat gtttttcatt atttggctgt tgtctttatt acgataaatc catcgtaatt
     1381 cgctggcggt aatactctga ccatagccct ctgacttgtt agctatgagt ttgagtgaat
     1441 tgacaagccc atccgcacaa aacataaccg ttctgttgcg aattaatgtt tgaaactcta
     1501 ggcctgcctt gcttgttttt gcccataact tgtctcgatt gtatatggaa aggtcggcca
     1561 tgatgacatt ataattattg tgatttttta agcactgttt aaattttgtt cctctttcat
     1621 gttcatttaa tgctcttttg ataagcgagt cttgcttata tttacggggg gttctggcgt
     1681 cccatactgc gtttgttaat gtattctgaa ttacgatatc ttttttggaa aagtcgttaa
     1741 ccagtttgat gctgttgaca agtttttctc tgggagagga tagcccatag acaatgtctc
     1801 ccggacgaaa atgcaaggca gcatccagtg cgttgccctg aaaagtgatg tttttttctt
     1861 taattacatt cggagagagt agtacatttt gtaaatcaag atccagtcca tcaggaaccc
     1921 cttcagctac gatgttccat gtagttttct gctccacatg aatggttaca gatctcagac
     1981 tctccgggag tttctccggc agtataatat tgctacatcc agtcagagat agcgtctgta
     2041 gtgacggtga tatcaggctg tcaattctgg cctgattttc tggggtgtag cgattgatgc
     2101 tgagagataa taacccgttt ggaacatttt ttatgccgtc tgtggcgtaa cctctgatct
     2161 caagatgacg gacactctct ggcactccag atatatggca gtggcaaacc gtcaggcatt
     2221 cgatgccttc agatatgatg tgtataaatg gtgaatcagt taattcaata tgtagatttt
     2281 ttactgtcga tggtatgtct gtgatttgta atttttcaca gttaatgacg gacaggtttt
     2341 gtgtattaac cggtaatcct gtaagggtgg tcaggttatt gcaattttcg attgtaactg
     2401 atgttatttc gtttggtaac tgtggcagat actcaatatc acaatctttg atatataaac
     2461 gaccaatggc ccgcgcttct tctatctgag ttggtgtgat ttcgcttttg atgcctgctg
     2521 atgtttctgc ggaattggag tagaatgtcc cggacggtcg taattgcgat gtaggaagca
     2581 ttgataaatt ccatttttag aagtaaataa cagtgtaagt tctatccata ctgctaatca
     2641 acaagctaat tttagcaacc agcaaagcca aggtgtcaat attagaaaag agagttatat
     2701 ctcaaaatca acagactgtt gattttaact tttgcgaata ctttctccag tccgtcatgc
     2761 atggattagg attgctcatt tttataccat ttgttgtttt ttatagcgat ttgctaaaag
     2821 ggagaagaaa aaacgttctc caaaattaga ttgcaactgt ttgattttat ggatagaggc
     2881 atgctgttta ctagacagta aaaagcatgc caaatttgct attaaccttt tgaatataaa
     2941 gcactaatta tctatttctg ttgccttctt attcagcaga cgagaagcgc gccagtaaac
     3001 cagcaacatc aggcccatta cgatagtcag cgtaatgctg gtggctgcac caaatggcca
     3061 gtcacgaata ttgaggaact gtaccttgat gacgttaccg atcagcaggt ttttcgcacc
     3121 gcccatcagg tcggacacat agaacaggcc catcgctggc agcatcacca gcagacatcc
     3181 ggcaataatc cccggcatcg tcagtgggat aatgatgcgg ataaaggtct gcaacttgct
     3241 ggcaccgaga tcgcgcgccg cctcaagcaa aggcttatcc agtttttcga tactggagta
     3301 cagcggcatc accataaacg gcagcagaat gtaaaccaga ccgataatca ccgcactggg
     3361 cgtgaacatg atacggattg gtgtgtcgat aacgcccagc cagagcaaaa actcgttgag
     3421 atagcctttg gtgctgagga aaattttcag cccgtagata cgaatcaatg agttggtcca
     3481 gaagggaaca atcagcagaa acagcagcag cggacgcacc ttgtgtggca gcttcgccag
     3541 aaaccaggca aacgggtagc ccagcaccag gcaggcgagg gtggcgatca gcgccatatt
     3601 cagcgagtgc aatagcactt caaaatagag cggatcgagc agacgcgtgt agttatccag
     3661 cgtaaagacc attttgacga aactggcgtc gtcgcgggtc aaaaagctgg tgccaatgat
     3721 catcaggttg ggcagaaaga caaacaacac aagccaaccg acaatagtga caatcactac
     3781 attctggaac ttacttgtgt tcttcatcag ccagtacgac ctcccagctt tctacccaat
     3841 taatggccat tttttggtcg agagagtggt caaagtcagg gtcgtcttca ttgaagaatt
     3901 cgctgaccat caccatcttg ccattttcca gttcaacaac cgactccagc gtcatgcctt
     3961 tgtagttacg ctcacgaacg taaccaatca gcccttcagc gtggttgtcg tcgttaatct
     4021 cttcaacacg taagtcttcc gggcgcagca gaacatgcag tttttgcccc ggttcaacgg
     4081 cgaagttaac gtagatatta cattcgcggc cttcaacgtt ggcgcgtacg cgctgctcgt
     4141 cgagtcgctc gatcaccgtg gcgttaaaca tattaatttc gccaatgaac ccggcaacaa
     4201 acaggttttt cggctcttcg tagatttcac gcggcgtgcc gtcttgctca atgcgaccat
     4261 cgcgcatcac cacaatcctg tctgacatgg tgagtgcttc ttcctggtcg tgagtcacga
     4321 agacgaatgt aatgccaagc ttacgctgta acgctttcag ctcgttctgc atttgcttgc
     4381 gcagtttgta atccagcgct gagagcgact catccagcaa caacagacga ggcttgttaa
     4441 ccaccgcgcg ggcaatggcg acgcgttgct gttgaccacc agagagctga tgcggtttac
     4501 gttgagcgaa ggtttccagt tgcaccatcc gcagggcttc catcacgcgg ggcgtaattt
     4561 cagcagcggg ggttttttgc atgcgcaacc caaaggccac attttcgaac acggtcatgt
     4621 gggggaaaag tgcgtagctt tggaaaacag tgttcacata gcggttttcc gccggaacgt
     4681 gggtgatgtc ctcgttatcc agcatgatgc gtccggaatc aacagtttcc agacctgcaa
     4741 tcaggcgaag aacggttgtt ttaccgcagc cagaagggcc aagcagcgtg aggaactcgc
     4801 cattgttgat agtcagatcc agctggggaa tgacctcttt accatcaaag catttgcgaa
     4861 ttcccgccaa ttgcaccagc ggtgaaagcg aactcggttg tttattcaat tttttactct
     4921 gtcccatgta aacgcaacgg atggcttacc gatgcggggt ttgtggttaa ccaccttggt
     4981 gactcttaat gagggcggta attctacggc aaaccgcttg aatcgccaat ctttgttgtg
     5041 aattactggc ttagctttat attcattaag gtaatgctga taaatattcc cgcttgcagg
     5101 ggtaaaagtg acctgacgca atatttgtct tttcttgctt cttaataatg ttgtcacaaa
     5161 aagtgagggt gactacatgg ataaactact tgagcgattt ttgaactacg tgtctctgga
     5221 tacccaatca aaagcagggg tgagacaggt tcccagcacg gaaggccaat ggaagttatt
     5281 gcatctgctg aaagagcagc tcgaagagat ggggcttatc aatgtgacct taagtgagaa
     5341 gggcactttg atggcgacgt taccggctaa cgtccctggc gatatcccgg cgattggctt
     5401 tatttctcat gtggatacct caccggattg cagcggcaaa aatgtgaatc cgcaaattgt
     5461 tgaaaactat cgcggtggcg atattgcgct gggtatcggc gatgaagttt tatcaccggt
     5521 tatgttcccg gtgctgcatc agctactggg tcagacgctg attactaccg atggtaaaac
     5581 cttgttaggt gccgatgaca aagccggtat tgcagaaatc atgaccgcgc tggcggtatt
     5641 gcaacagaaa aacattccgc atggtgatat tcgcgtcgcc tttaccccgg atgaagaagt
     5701 gggcaaaggg gcgaaacatt ttgatgttga tgccttcgat gcccgctggg cttacactgt
     5761 tgacggtggt ggcgtaggtg aactggagtt tgaaaacttc aacgccgcat cggtcaatat
     5821 caaaattgtc ggtaacaatg ttcatccggg cacggcgaaa ggagtgatgg taaatgcgct
     5881 gtcgctggcg gcacgtattc atgcggaagt tccggcggat gaaagcccgg aaatgacaga
     5941 aggctatgaa ggtttctatc acctggcgag catgaaaggc accgttgacc gagccgatat
     6001 gcactacatc atccgtgatt tcgaccgtaa acagtttgaa gcgcgtaaac gtaaaatgat
     6061 ggagatcgcc aaaaaagtgg gcaaagggtt acatcctgat tgctacattg aactggtgat
     6121 tgaagacagt tactacaata tgcgcgagaa agtggttgag catccgcata ttatcgatat
     6181 cgcccagcag gcgatgcgtg actgcaatat tgaaccggaa ctgaaaccga tccgcggcgg
     6241 taccgacggc gcgcagttgt cgtttatggg attaccgtgc ccgaacctgt tcactggcgg
     6301 ttacaactat catggtaagc atgagtttgt gactctggaa ggtatggaaa aagcggtgca
     6361 ggtgattgtc cgtattgccg agttaacggc gcaacggaag tcgtgattgc ccgatgcgta
     6421 tgtggactgc caattaccgg gatgaaaccg ttatattgat actggtcaac atacttatcg
     6481 tgcctacagg cgttcagtgc atgtaggcac gataaggcgc aatcagcgcc gcatccggct
     6541 taaatccaaa cttacccttc gaagaaccaa tacccgctat tgaccagcgc cgcgagcatc
     6601 gcgaggaatg acggatcttc cagcgcatcg ccaaaattct ccgcagtcag cgcaatgttg
     6661 ctggcgagtg catccagtgc cggacggtgc ggggaatcga tcttctcacc attggcatac
     6721 acgtcgtcgc caatgcgcaa tacgcgcaga ccacccaggc gcaccagcac ttcaccttgt
     6781 ttcagcgcat cgtagatttc atctggctga taaggcggtt ccggcggcgc gatatccagt
     6841 tcatgacgtg actgggatat aaactcgcca aaccattgct taaagtgttc cggctggttg
     6901 atcaattcga gcatcatctc acgcagttta tccatctctt gcggcagaac gtccgcagga
     6961 tgagcgcgag gtggaacatc cggatcgctg tagtagttgc cgcccagttc acgttgcagc
     7021 acataatcgg caaatccgct aattaattcc cgcgtatttg gcgcgcgaaa acccacggaa
     7081 tagttcatcg cattttccag cgcgtagcct tcatgcggga atcctggcgg aatataaaga
     7141 atatcgccag gctccagctc ttcatcgatg atggcttcga acggatcgac ctgtaacaga
     7201 tccgggtgcg gacagtgctg tttcatttgc agcttttcgc ccactcgcca gcgacgacga
     7261 ccggtaccct gaatgataaa cacgtcgtac tgatcgagat gcgggccgac gccgccgccc
     7321 ggtacagaaa aagagatcat cagatcatca atacgccagt ccggtagttc acggaacggt
     7381 cgcatcagcg cggcggtcgg ctcatgccag tggttcactg cctgtaccag taatgaccag
     7441 ttggtttcac cgagatgatc gtagctttcg aacgggccgt ggctgacctg ccatttgcca
     7501 tcctggtgac tgaccagtcg actgtcaact tcgctttcca tcgccagacc cgccaactcg
     7561 tctggagaga tcgggtcaat aaaattatta aagccgcgtt ttaacaccac cgggcgtttc
     7621 tgccagtgac gttcaagaaa atcgggccag ttaagagtga gttggtattc catgttaagc
     7681 atccacaggc tggtatctgc aaccgattat aacggatgct taacgtaatg cgtgaagtat
     7741 ggacatattt tatcaattag tgttttttgc cgtttcataa tgtttcagat tgttcattat
     7801 gtttttgatt ataaacagat ttgtgatttt ccttgttcca gcttgtttca cgttgtagcc
     7861 ctttgtgcaa cgatcggtgt agttaatggt gtagttaatt gtgatgaccg gaatcaaaat
     7921 tatgagcaga gcacttaaca aactgagcga tacacagctg aggaaaatca acggcacacc
     7981 cgcccaaaaa acagcctttc ttaatgacgg tggaaacctg agcgtcaggc attcaaccag
     8041 tggcctttta acctggtatt tcacttacag ggccggaacg ggaagggggg caccaccgga
     8101 acgcattaag ctgggaaatt atcctgatct gagcctgaaa tcagccaggg aaaaagccgc
     8161 ccagtgtcgc gcatggctgg cagaggggaa aaatccacgt catgagctta attacaccgt
     8221 acaggaagcg ttaaagccgg taacggttgg cgatgcgctc acctactggc ttgagtcgta
     8281 cgcaaaggaa aaccgcgtgg attatgccgc cctgaaaaag cgccttaata atcacgtaat
     8341 acagcacatt ggtgctatgc cgctggataa atgcgagcta cggcactggc tggcctgttt
     8401 tgaccaggtg gcaaagcgaa cgcctgttac tgccggattc ttgctacaga cgtgcaaaca
     8461 ggcgcttaag ttctgccgga ggcggcgcta tgcaatcagc aacgttcttg atgatatgag
     8521 tgtggcggac gttgggaaaa aaccggatat aagcgagcgt gtcttaagca ccaaagaact
     8581 gggcgaatta ttgcaggcac tggacaaaaa aatattctcc ccctactaca tcgcgttaat
     8641 ccgcctcctg attgtgttcg gatgccggac ggtagaactg aggttatcgg agatcagcga
     8701 gtgggatttt accgaaatgc tctggaccgt tccgaaggag cacagcaaaa cgaaggtagc
     8761 catattccgg cctataccgg aagcaatact gccgttcatc acgcagctgg tggagcagaa
     8821 caggcacacg ggcttattgc tgggggaagt gaaacaggag gccagcgtat cgcagtatgg
     8881 aagattagcg cacaggaggc ttaatcaccc tcactggtca ctgcatgaca tccggcgcac
     8941 ctttacaacc atgctgaacg atttaggcgt tgatccgcat gtcgtggagc agcttacagg
     9001 ccaccagatg ccaggaatgc agcgagttta taatcattcc cgttatctgg atgcgaaacg
     9061 caatgcgctg gatatgtgga cggagcggtt agggatactg gcgggaacac atgaaaacgt
     9121 aaccacgcta ccagtagcca gaagaaaata atttttttcg tgttttttca gtatgcgcat
     9181 actggatatg cgaacagata caacgtgcaa caatgagcaa caatgtggaa caactacgaa
     9241 caagaggcga aaaagcgtac ggatttataa gtagctgatt tttaatgtgt tactggtttt
     9301 tatacactag cgaatcactc tttaaagagc gagaaaaaag gcatggaaca atattttatg
     9361 cctttgtatt aacatgaatt taaaagattt tattcttact ttttcatttt ctgtaaatca
     9421 tacatcctca gtatttgcca ttcatcactc tagcgggaac aatacgacac aataagacac
     9481 ctgatgactc tttaagaaac gaaagggggc aatagtgtta agcactgatc ggtttatacg
     9541 tgaaaaagaa tgcgaaaaac taaccggcct tagccgtacg tgtcgctacc gcctggaaaa
     9601 ggccggacaa ttcccatcac gtcgtaaact tggcggtcgt tccgttggct ggtctttatc
     9661 cgaggttctg gcctggaagg atagctgcaa ggcagttcat taatcacgct ggcggcacac
     9721 agccgccaca catcaatcat ctgaacacag agctataacc atgaagattg aatatacgcc
     9781 agaacgtggg cggggattcg ttcgccctgg tgagactgga aaaccacaaa attggggttt
     9841 ttcaggcata aaaaaagcgg ccccgaaatg gagccgcctt tctgaacaga taacccgctg
     9901 cgccttatgt gtatatgatc ccaaacataa gcacggggat gatagccgct atcaggctgg
     9961 tgggcaatgc tatcagtctg gttcagttcg ttgccatacc tgcaatgggc gcgtttccct
    10021 gtactcttta aggaattgct caagggcaaa agcacatggc gcgaatcttt ctgattcatg
    10081 ctctatcttt ctgcgccgtc ttttccgtgc cggtgataat gttttggtca attctttatc
    10141 ggtcattgtg ttgtcctgca tagcaatgcg ccgtaatacc tcacaccacg gcgctgatag
    10201 tttttatcct ttgggttcta tgccgcgttg ctgtaattct ttgcggagaa cgcgtttaat
    10261 ccaagtggct aaagatgtgt cgccatcggc cttagctgct tcttctagct gcgctctgaa
    10321 gtcttccggt aatcgcattt ggtatggagg cgaacgtttt tctaatggtg ttgacatggt
    10381 aattaccctt tattactata cctacatggt aattaccatt gtacttgcca ttacacaaaa
    10441 atcaacgccc cgcagtgctg ggaacacata cggggcgtct aaccaacaac gtaaactagg
    10501 agccgttatg gttgccgtaa atcatatacc acaccttgta cacacacaaa cggcctttgt
    10561 gtggcgtttt ctggcactga gtgccggaga atctcaaatc atccacgtaa cagcctggac
    10621 ggaacgcgaa gcgcgtaacc gttgcccgtc cggttgtgtt gctgtatttg ccgcccgtat
    10681 tcgtcaggag gtgcgccatg attaatctgt cacttactga ccttaaccgc attcagtttc
    10741 gtgagaaatt cacagggcag ctactggtca atgtggataa aggtcgcgtg gtgtgtaatt
    10801 accacttacc agatgaagca attgtcgcca caagggaatc attacaggaa atcacaaaac
    10861 gtgccggaat gattaacacg aatattcagg ggatgcttta tgcacaataa aaccacaccg
    10921 gacgcagccg ccgaagccat caaaacgctg atgcacgcgc tgattgatat ttctgttatc
    10981 gcggacaggg cacataagca cgccaccagc gaaacagaat atgccggtgc tttcgttcct
    11041 cactcactgg ctgtcatgca atttagtgct gatacggcgc tgaatgaggc caaagctatc
    11101 ctgattgctg attgtgaaaa tgggggggtt atgcgtgatg atcgttttaa ttccctgaaa
    11161 caggaatttt ccggcgttcc tgatgatgcg gctgatgcgc tttcgtcaat gccagaactt
    11221 attagagcgg cttttttctt actttccacg agagaatata aatcaacggg gcttgatgta
    11281 ctgaatatcg ccgccgatta tgcggaatat gtggcagagg cgcgttatag aagaaaattt
    11341 cctgaggatg taagccatgc gtgatattta ccaccaaaca atagaccgcg catttcttgc
    11401 actttctcac agtgaaaaca tgatggaaat attgcgcata tggcttgaaa cacttggcga
    11461 caatgaacgc gacaaacaaa aatcaagaat tgccacggca ttaataacgc ttcttgagcc
    11521 tgtaataatg gaactgcaag aaatagatct attgcacgac agatataaag aacagcacac
    11581 cggagaataa aaataatgaa acttaaatat tctggcttaa ctgccagtgg caacactcac
    11641 cctaaattta cgcgcggtga tatttaccgc gaccagtacg gcggcacggt aatgattaag
    11701 ggcgtggaag aacggcgcgt aacctaccgc cgtgaaggtt acgaatatga ttgcgtgatg
    11761 cctgtttatc agttccggcg tgatttttct ctggtacaga ccgcgccgca taacgtgccc
    11821 accagcaacg ccagggcacg ggcaaacatc cagaagctga aaaccatggt taacggattc
    11881 aggggcaaga aatgaaaagc gcaccgaact taaaaaaaca gccttacgac aagatgaccg
    11941 aagtcattat ttttgcgggt agtgatgcct gggcacatgc gaaacagtgg caggaacagg
    12001 acgggcgact ggctggcgat aatgtgcctc ccgttgtgct ggctgatgat caactggatg
    12061 aactggcaga cctgagaatc atcgacgagg ggcgctattg tgtccggctg tacaaggcag
    12121 gccacatcag gccatcaaat attaatgcca ttgcgcacaa gctggcggcg gcgggtgtaa
    12181 ctgatgcgaa ttattacccc gaagggatgc acagccatat gcgggagaac tggcgcgaat
    12241 acctggaacg ggtgcgcggg aaagagccgg cggaagaaaa aaaccaccag cgaaaaacca
    12301 cgctaccgat gagcgttgga tctaccggat acgacacgca actggattac gtggttaagg
    12361 ggattattcc ggcggtatcg ctatgcagca tatacggggc tagcgggtcc tataaatcat
    12421 tccttgccgg atcgtgggcg tgccatgttg ccactggtcg ccagtgggga ggccgcaggg
    12481 ttgcacatgg tgcggttctc tatgtggttg gtgaaggcgg tataggtgtt ccgcgtcgtg
    12541 taaaagcctg ggaggttgtg cacgatgagc aggtgaaaaa tctgtatctg gtaaaccgcc
    12601 ccatctttcc ggctgccccg cttgatgttg atgaaatggt tatcgctgcc cgtcaggtgg
    12661 agcgggaaac gggtaaacct gtacgcatga ttattctaga tacgctggcg cgttgctttg
    12721 gtgggaatga tgaaaatgat tcccgtgata tgggggcgtt tatccgtggt tgtgacgaac
    12781 tgaaacgacg cacaggggcc acggtgctgg tggttcacca ttccggcaag gatgagacga
    12841 aaggcgcgcg cggttccagt gcatttcgtg cttcgctgga tgctgaatac cggatacgca
    12901 gggaggacgc aggaagcgaa gcgctggtta tctcatgcac caaaatgaag gacgcggagg
    12961 aactcaaaga agccgcatat gacttacgcg tggtggagct ttttaccgac gctgacggtg
    13021 aattaatcac gtcgctggtg gtggtggatg atccgcgccc tcctgttgaa ctggagcgca
    13081 tcgaggaggc agggaacaag acggaaaacc ataccgcgct atgggggtgc atccgttcac
    13141 gcacacagaa cggcgacaag tgcacgatcc cgctgttacg tgatgacatg aaaaagctgg
    13201 ggtatgaaat gaaaaacttc cggcgctggc tgtacaagct ggaaaaagat ggggttattc
    13261 gtatcgatgg ggatgatgta gcgccgctat aaaagtgagg agcaaaagcg agggggatag
    13321 aaagagggcc aaaattagcc cgctctccct cacttttcga cctgtataca tcctcaaaag
    13381 tgaggggtaa aaaaatactt atgaaacaca cacatagaaa aaccgaaaat cccaactgcg
    13441 acgaagtgag acgcttgaaa aagtgaggcg aaaaagtgag aggttgcgag aaatgaccca
    13501 aaaacgcaga gacagaacag agccaaaata taaagcgtta gacatgactg agcacacctt
    13561 aaaggtggca atcagaacga tagaccgcca cacgcgggaa ggatacgcga aggaacatcc
    13621 cgacctgata agcgcattca tgaccacggc ggcggcaaac tttgccacgc tgacagaacg
    13681 ggagattgcc gaagcggaac aggtaacaac catcaacgtt aaaaccggag aggtggaatt
    13741 atgacggcac agatagccgc ttacgggcgg ctggtggacg acccgcaggt aaaacagacc
    13801 agcaagggca caccgatgac gctggcgcgt atggcggtat ctttgccatg cagccaggca
    13861 caggacggac aggcgacgtt atggttatcg gtcatcgcat ttggcaaaca ggccgacttc
    13921 ctggctaaac atcaaaaagg cgatgttgcc agcgtatccg gcacgatgca ggtaagccag
    13981 tggaccggac agaacggaga aacgcggcag ggctggcagg ttatcgcaga cagcgtaatc
    14041 agtgcccgcg cggcacgtcc aggcgggaac agacgcaaaa ccacaggcac acagggtaat
    14101 cagccaccag cgggaggcga tgacccttac ggtgatgata tttcgttctg agggggtgac
    14161 gatggtacat gaccgcatag cggaggaact ggaggcgaaa ggcttttacc ggagggcggc
    14221 ggcgcgatgg ggtgaagtca tgcagctggt ggagacagac aaggaacggc atcacatcac
    14281 gatgcgacgg ctggaatgtt caaggaaggc acagagggca ccggagccgc cggataattt
    14341 cggagacctg aaaaaggcag tcgatcgcac ttatgccgaa atgggtatag atggtgctgg
    14401 tgatgaaata tggcgcaatt accaggacag ctaatcaaac agccggagaa atccggcttt
    14461 ttttgcacca gttgaaacgg tatggcgcat taccgggttt tcgtcacggt caggcatagt
    14521 tactatctga aacaaacaga cacaacagag gaaaaaaaca atgccgatga aatttgatga
    14581 gatattaaaa cagcgtgata aataccatgc tgacaacatg gagacgatga gcatcaatga
    14641 ttaccgcgca ttcctggaga cgggcgcact gattgaaaag gatcagcatg gttttgtgag
    14701 atgtgctcta tccggtgaaa tgctggcggt aaatcctgaa cagatagatg cattgataga
    14761 atttctgaaa gagatcagag actgagccag cacacagcac acatagccgg agcaatccgg
    14821 cttttttgcg cccaaaaaaa gcccgataag gtcagagggt tcttatcggg cttttgcata
    14881 tgaggttttt ggatgcactg gcgttcgtga tcgggacaat catttcataa tttgcaacat
    14941 aactcaatat cattgcataa aatgcaattc tgattataat caggactgga taaacatcca
    15001 gttatgattt tttaagtcga agaggaattt cttactatgg ctgaagagaa aaaaggcggt
    15061 gtttcggtgt acataagccc cgacatcgtg aaggcgctca aggaacgcca ccagcagaac
    15121 gtaaaagcag gcattgcggc aggacttgat ccgctggcga tggttgagcc gtcaacaggc
    15181 tggcaggtcc gcgcctattt acgcgcggcg ctgggcatga atcagacgca tggaggtgaa
    15241 taatggcagg caaagcaacg gcacttaaca ctaaccagct tttcgcgtac ctgaatcgcg
    15301 gggatattgc agaatttaaa ttcagtccgc tgtttaccac gctgtttttc ccgaacgtgg
    15361 cgaccttcaa cacccagaac atcatgctgg acaccctgga cattgaagag gttacgatgt
    15421 cagcgttctg ttcgcctatg gtgggtagtc aggtccagcg cgataaaggg tacgaaacca
    15481 gcacgattaa accaggctac atgaaaccca aacacgaaat cgatccaaca aaaaccatca
    15541 tgcgcatggc tggagaagat ccggcacagc ttaacgatcc tacctatcgc cgtatgcgcc
    15601 tgattactgg caacatgcgc cgccagataa acgccattaa ggcacgcgtg gaatggctgg
    15661 cggtgaatgc ggtaacgacc ggaaaaaacc tcattgaggg cgaaggcata gaacgctatg
    15721 aaatcgactg gaagatcccg gaaagcaata tcatagaaca ggctgacggt aaaaaatggt
    15781 ctgagctgga taaagaaaca cacgatccaa tctatgacat cgagctttat gctgatcagg
    15841 gcaattgccc cgcaaacgtc atgattatgg gcgctgaagt atggcgcacg ttacgcagct
    15901 ttaaaaaatt ccgcgaactg tacgaccttt cccgtggttc agaatccgcc gcagaactgg
    15961 cctgtaaaaa cctgggtgaa gtggtgagct ttaaaggcta tctgggcgat attgcccttg
    16021 tcgtctattc cggcaaatac actgacagcg acggcaccga aaaatatttc cttgagcctg
    16081 atttgctggt cctgggtaac accaacaata aagggctggt ggcctatggt gcgattatgg
    16141 atcaggaagc ggtcagaacg ggcgcaacgc aaaacatgtt ttacccgaaa aactggattg
    16201 aggacggcga tccggcgatt gagtacgtgc agacgcacag cgcaccgcag ccggtaccgg
    16261 cagatattcg caaatttgtt accgtcaaaa ttggttaacg gggggattct atggacactc
    16321 catacattga gttatttgca ggtagtcagc aggtatccac gacgctggta cattttgctg
    16381 ctgatgctgg cgttattcag gaatttaccc cgctgatgct ggcggaaaat ggcgagttta
    16441 agccgtggga tggtcaggaa tctggcaagg ctgtttatct gacctcgtac cccgtggaca
    16501 cgtcgacgca gaaatcagca cagtgctaca agacggggat attcaatatt gccgccgtta
    16561 actggcctga gagcgccgac accgacgcga aaaaatgcgc cgcctttgcg ggttctggcg
    16621 tatccgttca gccgctggcg cgataagcag ggggaacgat ggcaacgaat gaaagtatca
    16681 tgacgctacc gctggcgagt aaatttaaag ccgaagcgcg ggcaatggct gacagaggtt
    16741 tatcaaccta cgaggccgta tatcaactta acagcctgga agagaaggac aagccgcgcg
    16801 ctgatgcgat tatggcgctt catgaatctg acgactatca gccgctgtta cgtgcaatgg
    16861 caaacgtgcc ttgtattgat gttgatacgg ctaaaaacat cctgaacatg accatagagc
    16921 aggaacgccc gaaggttgca ccagagctta ccgcagcctt tgaaaacttt atggacatgc
    16981 acagcccaca agccgtatca gctggcatgg catacgatgg cagaaacccg ggcgatgacg
    17041 gcgacatcga tcgcatactg aaaaccatct gagacaaggc cggagaaatc cggctttttt
    17101 gcggggcctt tccggcatat ggacctgtta cggggcggcg acctcgcgga ttttcgctat
    17161 ttatgagcct tttcagggtg gtggtggtgg ttttgttgtt tgatctatct ttatgaatga
    17221 aaagggaaag atgcaagcaa tacaccaacc tgaagcagta attaagttgg tgtattaatg
    17281 aaatctcatc tgatgaacaa aaaaaacatg gcgaaaagct gccgtgtaag tgcgacagcg
    17341 ttcgacaagt ggggagtaac tcccgttgag cgtaaaggcc gtgaggcgtt ttatgatgtt
    17401 gccagcgtga tagaaaatag ggttaacaat gcaattagcc agcttataaa cgacaaaggc
    17461 gagattgacg atgatgaact tttacgagtc aggatcagat tactgacagc acaggcagag
    17521 gcgcaggaac ttaaaaacga tcgcgatcgc ggtgacgtga ttgatactga gttttgcctg
    17581 tacgcgcttt caaagctggc gagtcagatt tcatcaatca tggacagcct cccgcttact
    17641 atgcaaagga gcttcccaca gattaccccc gccatgctgg acaggcttaa aagggaagtg
    17701 gttaaagcct gtaatgccag tgccagagtt gccgacaacc tcccacagat actggctgat
    17761 tacttgaaag aaacaaccgg aaacgtaccg gaaaagttgc tacagaagaa aggcgagtaa
    17821 cagacgcgca attattgaac aaaaatgaga aaggatctga taatggcatt ttctggctaa
    17881 aatgcctcgt gtcagatcct ttcttttttt taatattttc attgttaaac agtctgttac
    17941 agagaagaag atcggatctg acttttgccg gaaaattctc ataaacagat aaaaaccgcg
    18001 aggtcgccgc cccgtaacgg gccataattc caggaaggac ccgacgacac cagactatca
    18061 gagcgatggg ggcacaatga cagaagccga actactggga ttaatccgcc gcgtcgccgg
    18121 aatcagccag cagcatgacg aacaggccac acagccggac agcgtgaccg ctgaaaatta
    18181 tgtgcgtgtt gtagcggaag tgatgcgccg tgatggtata gaacttaatg gcgttgatat
    18241 gcgcaacata cggaaccgcg ttcttgaaat gctggcctac aatcgccgcg ttgaactgta
    18301 tcgggagaaa gaaaaaataa cgtaccactg gaagaagccg gaacggctgc ggcggtaact
    18361 tgctgatatt cccgataacg caaaattgcg ctggctacat ccctcacatc tgaggactgt
    18421 taatcagaag gccggaatat attcacaaac tacgaaattt tcgtagttta attaatggtc
    18481 agattaatcg ctaacccgct gatattttcg gaaacctcaa tttgaggaag tcggaagcgt
    18541 cacccttcat attctgaatg gtcataatga ccacgcagac catcaaggca accacaacga
    18601 acatcgttac ggttggagct tttaccccac tgggggaaag gtattacgat aatcataaca
    18661 cctaccgaac aggtaaaacc caccagcctg attaacagtt aaccggaaaa aaaatccggt
    18721 agccccgtta tggggatccc catatcgaca ttaacgcccc tcatgaattg gtgcgcttcc
    18781 ccctggaaag attacccgcc tgtatatttc ttgtgtctat ttgttccacg ttgtttcata
    18841 cagtgcaccg aacggtgtag ttaatggtgt agtcaatttg ctgtttttag cactttttgc
    18901 agtgtgggtt gtttaattaa atcaatgggt tgccttgctg tggtgataaa aggacatatt
    18961 tattcatctt tcggcgcaga atgctggcga ccaaaaatca cctccatccg cgcaccgccc
    19021 agcatgctct ctccggcgac gattttaccc tcatattgct cggtgatttc gcgggctacc
    19081 gccagcccta caccttgccc agggcgtaaa gtatcaaccc gttgaccacg gtcgaaaatg
    19141 acctctcgct tgcttaatgg aataccgggg ccatcatcct cgaccacaat atagagatgc
    19201 tcgtcggttt gccttgcaga aatttcgaca aactcgaggc aatatttaca ggcattatcc
    19261 agcacgttgc ccatcacctc gacaaaatcg ttctgctcac cgacaaagct gatctctggc
    19321 gaaatatcga gagagatatt gacccctttg cgttgataca ctttgttcag cgctgaggtg
    19381 agattgtcca gcagtggggc gaccggatgc agctcgcggc tgagcaatgt cccgccgcgc
    19441 atactggcac gatgcaggta gtagccaatt tgctgtgaaa tgcggctgat ttgctccagc
    19501 attaccggct cagcatcact gacgctcatc ttttcactac gcagagaacg cagcgtactt
    19561 tgcagcaccg ccagtggcgt tttcagacta tgggtcaggt cggtgagcgt cgtacggtat
    19621 ttgtcgtaac gttcgcgttc actttttaac aatcggttca ggtttcgtac cagactggtc
    19681 agttctcgcg ttgtggctgg attgagcaat tcgcggttat gttcttctag ttcgcggact
    19741 tcttttgcca gggcttcgat ggggcgtaaa ctccaccagg cggcgaccca cagcagcggg
    19801 atcactaaca gcagattggc tgagagcaca tagataaacc agctccagac catataggaa
    19861 ctttttagct ccaccggaat ggtatccacc accacaaagg ttaattttgg catccgcgat
    19921 gttgccgggt agacgtttac tgccaccgag tgggtcatct ccgcgtcgtc atcatcttcc
    19981 cgcacttcct gcaactgttg ctgtatcgaa tgatctccac ttagcaagag gctggtatcg
    20041 ttaacatccg cttcaatttc atgaaaacca ttcgatttca gccagtcagg ctggatcatc
    20101 ttcatcagcc agggcacgtc acgttgcgcc cataaaagct gcccgttctc atcataaatt
    20161 agcgtcatgg tggggctttg cttgtcgata ttttcgggta actcgacatg caacttattg
    20221 ttttcccact tcgcaagggt atagaacaga ttgctctcgc cacgtaacag ccgaaacgta
    20281 gttttatcga aactgacgct ataaccgatc agcgcgacca ttccgtaggc aagcgaaagc
    20341 accagtacta ccgcagccgt tgccaacaga aaacgtaccc gcagtgagag cgggaaaaaa
    20401 agacgcagta attttttcat cagcgcaatt cgaacaaata gccctggccg cgaacggtag
    20461 taatcacttc ttgcggatat tgtgcctgaa tttttttgcg cagacgtccc atcagtacat
    20521 caatggtatg gctttcccgc agctccgcat ccggatagag ttggagcatt aacgaatctt
    20581 tgctcaccac tttgccatta ttgcgtatca acgtttccat aatggtgtat tcgaacgcgg
    20641 tcagtttgat cacttcgtca ttaatagata attcacggcg agagagatca acctgaaacg
    20701 ggggtagcga aatgacctgt gaagccaggc cgctattacg ccgcattaat gcctgcattc
    20761 gcgccatcac ctcttcaata tgaaacggtt tagtcacgta atcatcagca ccggcactta
    20821 atacttcgac tttgtcctgc cagctttcac gggcggttaa taccagaatc ggcagtgaaa
    20881 catcattgct acgccagcgg cgaatcagtg acagaccgtc ctcgtcaggc aatccgagat
    20941 cgacaatcgc aatatccggt aaatgttcat tgagataata atcggcttct ttggcatctt
    21001 ctgcgtcatc gacctgatga ccagcatcct gaatctgaac tttaaggtgg tgacgtaaca
    21061 acgcattgtc ttcaacaacc agtacgcgca tttttatttc tccctgtctt aattattaaa
    21121 atagtgtaac gcgattatgt gtttatgggg gtaaacatta aataaaccag cggggagggg
    21181 aggtaaagtg aaaaaagaaa aagtggataa ttttaataaa caggccggac agcatcgcca
    21241 tccggcactg atacgagttt atttcagctc atcaaccatg gtgatagctc gaccaatata
    21301 gttagccggc gtcatcgctt tcaggcgggc tttctcttct tctggcaacg ccagaccatc
    21361 gataaactgc ttcatgcctt cggcgtcaac gcgcttaccg cgagtcagct ctttcagctt
    21421 ctcgtacggt ttttcgatgc catagcgacg cataactgtc tggattggct cagccagcac
    21481 ttcccagttg tgatccagct catccagcag atggtcacgg tttacttcca gtttgctcac
    21541 gcctttcagg gtggattgat acgcaatcag cgcataaccg atacccacgc cgaggttacg
    21601 cagcacggtg gagtcggtca ggtcacgctg ccagcgggaa accggcagtt tgcttgccag
    21661 atgctgcaat accgcgttgg aaaggcccag attcccttcg gagttttcga agtcaatcgg
    21721 gttaacttta tgcggcatgg tggaagaacc aatctcacca gcaatggttt tctgtttgaa
    21781 gtggttaagg gcgatataac cccagacgtc acggtcaaag tcgatcagaa tggtgttgaa
    21841 gcgcgcaacg caatcaaaca attcggcaat gtagtcgtgc ggttcgatct gggtggtgta
    21901 cgggttccac tgaataccca gcgaggtgac gaactcttcg ctgaactgat gccagtcaac
    21961 ttccgggtaa gcggcgatat gggcgttata gttaccgacc gcgccgttga ttttgccgag
    22021 gatctccacc tgattaagct ggcggtactg gcgctccata cggtaggcga cgtttgccat
    22081 ctctttaccg atggttgacg gcgtggctgg ctgaccgtgg gtacgagaca gcagcgggat
    22141 atcgcggtac tgaacggcga gatctttaat gccatcaatc agttgacgcc agtacggcag
    22201 gatcacttca tcacgcgcgg ttttcagcat taatgcgtgg gagaggttat tgatatcttc
    22261 cgaagtacag gcaaagtgga tgaattcaga aaccgcgtgc agttccggga tctccgccac
    22321 tttttctttc aggaaatact caaccgcttt aacgtcgtgg ttagtggtac gctcgatggt
    22381 tttgatgcgt gcggcatctt cttcgctgaa attggcgaca attgcatcaa ggtaaccgat
    22441 tgcgtcggca gcaaaagcag gaacttcctt gatcgctgcg tgcgcggcca gtttttgcag
    22501 ccaacgtact tcaacttgta cacggaattt cagcaaacca tattcgctga aaatcccgcg
    22561 cagcgcgctg actttatcgc cgtagcgtcc atcgacaggg gaaacggcgg tcagtgagga
    22621 taattccata gatcacaact ccggggttaa atgagcaaga atttgttttg cctgagtggt
    22681 caggcgatta cgagaaaaca tcagttgcag acgtccgccg ccgacctggt gccagagcac
    22741 ggcggcgcga atgcctgcca gcagggttgc gcgaactttc gcctgcactt gtgggctttg
    22801 cagtacagca ggggaaccgg tgacctgaat gcgcgggcca agcgggctga tcacatcaac
    22861 atagatagca gccatcgcgc tcatcagcgt ttcggactgt aaatcgaagt gttcgagctg
    22921 gcgttgcagg ccgttgatcc ggttgcccag agtgtcgagc gcgcctttcg ctgaggagag
    22981 tttgcgctca agcaccatca agctgagtgt gtagcgggtt aattcggcgt ttaagccctg
    23041 gcgactgctg gcattgagca cgccgagcag ggtttccagc ccgacgcgca ggttggcttc
    23101 gctaccgcca aaaaccgcca gcgtcgagct ggggttcata tcaataatac tgttgagtga
    23161 gacgtgtagc gcatcggcat cacaatgccc ctggtgagcg agtcgttgca ctaggcgtgc
    23221 cgactgacaa ataccggcca gggcgagggt gatgtcatag taattctttg ccacgttcac
    23281 tgcttccttg ttttaagtaa agataataat cagaccggca gcggcagacg ctgctcaata
    23341 ataccgccac cgaggcacac ttcaccgtta tagaagacgg cagactggcc cggcgtcacg
    23401 gcggcaaccg gttcatcgaa aatcacttca atgcgatcat cgtccagcgc cttgacggtg
    23461 caagggatat cggtctggcg atagcgggtt tttaccgtgc aacgcatagt gccggtgaat
    23521 ggttcgcgat cgacccagtg caactgctgg gcaatcaacc cgacagacat cagccgcggg
    23581 tgttcatggc cctgagcgac aaccagaatg ttgttttcga cgtctttgtc caccacatac
    23641 cacggttctt cggtaccttc tttggtgcca ccgataccca gacctttacg ctgaccgaga
    23701 gtgtgataca tcagcccctg gtgctcacca atttcatcgc catcgacggt aatgattttg
    23761 cccggttgcg ccgggagata acggcccagg aactcgcgga atttacgttc gccaatgaag
    23821 caaatgccgg tagagtcttt tttcttcgcg gtgaccagac caagatcttc agcaatctta
    23881 cgcacctgcg gtttttccag ttcgccgacc gggaacaggc tttgcgcaat ctgctcatgg
    23941 ctgagcgtat aaaggaagta gctctggtct ttattgctgt ccagaccacg cagcaggcgg
    24001 ctcttgccat cgacatcggc ccgacgtacg taatgaccgg tagcgatata atcgccacct
    24061 aaatcttcgg cggcaaattc gaggaaggct ttaaatttga tctctttgtt gcacagaata
    24121 tccggattcg gcgtgcgacc ggctttatat tcggcaagga acagttcgaa gacgttgtcc
    24181 cagtactcgg cagcaaagtt aacggtgtgc agttcaatgc cgagcttgtc gcagacagcc
    24241 tgggcatcag ccagatccgc tgccgctgtg caatattcct caccgtcgtc ttcttcccag
    24301 ttcttcataa acaggccttc aacctgatat ccctgttgtt gcaacagcca ggcagaaacg
    24361 gaggaatcga caccgccgga catgccgacg attacttttt ttgcggtttc agacattgga
    24421 tcactcacga cattgaactt caaggcggcg tattctatca cgcagccttt tagatgacac
    24481 cctttgtaaa aggccagtta aaatcgccaa tcatctccag cggatagcgt tgcccgcttt
    24541 gataacaacg gatactttcc gccaccagcg gcgaacgaag atttgacgcc tttaaaatct
    24601 cttcggcgct gacccaacga cagcagtcga tatcgctgtc atgaggctgg gtcgggcata
    24661 tttgctcaag ctcaatggca aagaggaaac gcaaaaacgg cgttttatct ggcgcaatcc
    24721 actgatgcat acgaataaag tgttgcggct gcgcgctgat gccggtttct tcccacagtt
    24781 cacgggcggc ggcttccact aaggtttcat cggcttccag atgcccggca ggttggttcc
    24841 ataacgcttt accattaatc gtctcttcaa cgactaaaaa tttgccttct gcgtgcacca
    24901 cgcaagcaac ggtaacgtgc ggtttaaaca ttttgcatcc ttaatctgtc acttctcgcc
    24961 attcgccatt ggcaagatta tccaaagagt aatctcccat cgcatagcga atcagtcgca
    25021 gcgtggggaa accaacatgg gcggtcatgc ggcgcacctg gcgattacgt ccttcatata
    25081 aggtgatctt cagccagctg gtgggaatac ttttgcgttc acgaattggt ggattccgcg
    25141 gccataacca cgctggttcg tcaaccagct ccgcgccggc gggcagggta gggccatcat
    25201 ttaaggttac gccattgcgc aaggcttcaa gtgcgtcttg tgtgggaata ccttccacct
    25261 gcacataata gattttcccg gtgcgtttac ccggctgggt taaacgcgcc tgcaacgcgc
    25321 cgttattggt cagcaccagt aacccttcgc tatcgcggtc aagacgacct gctgcataaa
    25381 cgccctgaac cgggatgaat tcttttaatg ttttgcgtcc ggcttcatcg gtgaactgcg
    25441 gaagaacatc gtagggttta ttgaacagga tcacacgcgt gggctggttt tcaggtttac
    25501 gcctggtaga acgttgcgag ctgaatcgct taacctggtg atttctaaaa gaagtttttt
    25561 gcatggtatt ttcagagatt atgaattgcc gcattatagc ctaataacgc gcatctttca
    25621 tgacggcaaa caatagggta gtattgacaa gccaattaca aatcattaac aaaaaattgc
    25681 tctaaagcat ccgtatcgca ggacgcaaac gcatatgcaa cgtggtggca gacgagcaaa
    25741 ccagtagcgc tcgaaggaga ggtgaatgga aagtaaagta gttgttccgg cacaaggcaa
    25801 gaagatcacc ctgcaaaacg gcaaactcaa cgttcctgaa aatccgatta tcccttacat
    25861 tgaaggtgat ggaatcggtg tagatgtaac cccagccatg ctgaaagtgg tcgacgctgc
    25921 agtcgagaaa gcctataaag gcgagcgtaa aatctcctgg atggaaattt acaccggtga
    25981 aaaatccaca caggtttatg gtcaggatgt ctggctgcct gctgaaaccc ttgatctgat
    26041 tcgtgaatat cgcgttgcta ttaaaggtcc gctgaccact cctgttggtg gcggtattcg
    26101 ctctctgaac gttgccctgc gccaggaact ggatctctac atctgcctgc gtccggtacg
    26161 ttactatcaa ggcactccaa gcccggttaa acaccctgaa ctgaccgata tggttatctt
    26221 ccgtgaaaac tcggaagaca tttatgcggg tatcgaatgg aaagcagact ctgccgacgc
    26281 cgagaaagtg attaaattcc tgcgtgaaga gatgggcgtg aagaaaattc gcttcccgga
    26341 acattgcggt atcggtatca agccgtgttc tgaagaaggc accaaacgtc tggtccgtgc
    26401 cgcgattgaa tacgcaattg ccaacgaccg tgactctgtg accctggtgc acaaaggcaa
    26461 catcatgaag ttcaccgaag gcgcgtttaa agactggggc taccagctgg cgcgtgaaga
    26521 gtttggcggt gaactgatcg acggcggccc gtggctgaaa gttaaaaacc cgaacaccgg
    26581 caaagagatc gtcattaaag acgtgattgc tgatgcattc ctgcaacaga tcctgctgcg
    26641 tccggctgaa tatgatgtta tcgcctgtat gaacctgaac ggtgactaca tttctgacgc
    26701 cctggcagcg caggttggcg gtatcggtat cgcccctggt gcaaacatcg gtgacgaatg
    26761 cgccctgttt gaagccaccc acggtactgc gccgaaatac gccggtcagg acaaagtaaa
    26821 ccctggctct attattctct ccgctgagat gatgttacgc catatgggct ggactgaagc
    26881 cgcagacctg attgttaaag gtatggaagg cgcaatcaat gcgaagaccg taacctatga
    26941 cttcgagcgt ctgatggaag gcgctaaact gctgaaatgt tcagaatttg gtgatgcgat
    27001 catcaagaac atgtaatcac tacatgtgtt taatattgca acgggcgtat aacacgcccg
    27061 ttgttttatt tatgtaggta ttattaatag catatcgagc atatttatat aaaacccttt
    27121 acttgagccc atatgggcat atttttataa tgcaactatt atgcaaacat ttatttgtta
    27181 tttttctttc tcctggagga cactcttgac tgcttttgag tagactccat aaatccttgt
    27241 t
//
LOCUS       DAZFYC010000066        27229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFYC010000066 DAZFYC010000000
VERSION     DAZFYC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.066"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            432..2855
                     /gene="pgaA"
                     /locus_tag="ACHKW5_002979"
     CDS             432..2855
                     /gene="pgaA"
                     /locus_tag="ACHKW5_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO7512334.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2864..4882
                     /gene="pgaB"
                     /locus_tag="ACHKW5_002980"
     CDS             2864..4882
                     /gene="pgaB"
                     /locus_tag="ACHKW5_002980"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO7512335.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4875..6200
                     /gene="pgaC"
                     /locus_tag="ACHKW5_002981"
     CDS             4875..6200
                     /gene="pgaC"
                     /locus_tag="ACHKW5_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO7512336.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6202..6615
                     /gene="pgaD"
                     /locus_tag="ACHKW5_002982"
     CDS             6202..6615
                     /gene="pgaD"
                     /locus_tag="ACHKW5_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO7512337.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6665..7453)
                     /gene="phoH"
                     /locus_tag="ACHKW5_002983"
                     /pseudo
     CDS             complement(6665..7453)
                     /gene="phoH"
                     /locus_tag="ACHKW5_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
     gene            complement(8072..9343)
                     /gene="efeB"
                     /locus_tag="ACHKW5_002984"
     CDS             complement(8072..9343)
                     /gene="efeB"
                     /locus_tag="ACHKW5_002984"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO7512338.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9349..10476)
                     /gene="efeO"
                     /locus_tag="ACHKW5_002985"
     CDS             complement(9349..10476)
                     /gene="efeO"
                     /locus_tag="ACHKW5_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO7512339.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10534..11364)
                     /gene="efeU"
                     /locus_tag="ACHKW5_002986"
     CDS             complement(10534..11364)
                     /gene="efeU"
                     /locus_tag="ACHKW5_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO7512340.1"
                     /translation="MFVPFLIMLHEGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11907..13415)
                     /gene="putP"
                     /locus_tag="ACHKW5_002987"
     CDS             complement(11907..13415)
                     /gene="putP"
                     /locus_tag="ACHKW5_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO7512341.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            13837..17799
                     /gene="putA"
                     /locus_tag="ACHKW5_002988"
     CDS             13837..17799
                     /gene="putA"
                     /locus_tag="ACHKW5_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7512342.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAAQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLARRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17839..18477)
                     /gene="rutR"
                     /locus_tag="ACHKW5_002989"
     CDS             complement(17839..18477)
                     /gene="rutR"
                     /locus_tag="ACHKW5_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO7512343.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18765..19856
                     /gene="rutA"
                     /locus_tag="ACHKW5_002990"
     CDS             18765..19856
                     /gene="rutA"
                     /locus_tag="ACHKW5_002990"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO7512344.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19856..20548
                     /gene="rutB"
                     /locus_tag="ACHKW5_002991"
     CDS             19856..20548
                     /gene="rutB"
                     /locus_tag="ACHKW5_002991"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO7512345.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20560..20946
                     /gene="rutC"
                     /locus_tag="ACHKW5_002992"
     CDS             20560..20946
                     /gene="rutC"
                     /locus_tag="ACHKW5_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO7512346.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20954..21754
                     /gene="rutD"
                     /locus_tag="ACHKW5_002993"
     CDS             20954..21754
                     /gene="rutD"
                     /locus_tag="ACHKW5_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO7512347.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLQQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21764..22354
                     /gene="rutE"
                     /locus_tag="ACHKW5_002994"
     CDS             21764..22354
                     /gene="rutE"
                     /locus_tag="ACHKW5_002994"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO7512348.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HSDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22365..22859
                     /gene="rutF"
                     /locus_tag="ACHKW5_002995"
     CDS             22365..22859
                     /gene="rutF"
                     /locus_tag="ACHKW5_002995"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO7512349.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22880..24208
                     /gene="rutG"
                     /locus_tag="ACHKW5_002996"
     CDS             22880..24208
                     /gene="rutG"
                     /locus_tag="ACHKW5_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO7512350.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGMNPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(24291..24464)
                     /gene="ymdF"
                     /locus_tag="ACHKW5_002997"
     CDS             complement(24291..24464)
                     /gene="ymdF"
                     /locus_tag="ACHKW5_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7512351.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24837..25433
                     /gene="wrbA"
                     /locus_tag="ACHKW5_002998"
     CDS             24837..25433
                     /gene="wrbA"
                     /locus_tag="ACHKW5_002998"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7512352.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25454..25681
                     /gene="yccJ"
                     /locus_tag="ACHKW5_002999"
     CDS             25454..25681
                     /gene="yccJ"
                     /locus_tag="ACHKW5_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO7512353.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25719..26960)
                     /gene="agp"
                     /locus_tag="ACHKW5_003000"
     CDS             complement(25719..26960)
                     /gene="agp"
                     /locus_tag="ACHKW5_003000"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO7512354.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         6656 a         7052 c         6899 g         6622 t
ORIGIN      
        1 gcaacaagcc aggcttgaaa atacttataa atggttatag aaaatcctta taaaacatta
       61 attcatagat acattaggaa taacaattaa atccgtgagt gccgtagcgc agcctttcat
      121 caggactttc gaaaaatccg aaatcatgca tcggaattta ctgatttaat tattttaatc
      181 ctaatttatt ttgaaaaagg cattgggatt tatgccgtat tcctgaagat cctcatcatt
      241 ggaatggatt ttcgggcgag aaaaggattt tatatggaca ctctgctcat catttcttct
      301 tctcatcatc aacaattcac gtctctcttc cgcgtttaat aacggattat gaggtgcaaa
      361 aatatctttc ttttcagtta cctgtaatta gatacagaga gagattttgg caatacatgg
      421 agtaatacag gatgtattca agtagcagaa aaaggtgccc gaaaaccaaa tgggctttga
      481 aacttcttac tgccgcattt ttagcagcga gtcccgcggc gaagagtgct gttaataacg
      541 cctatgatgc attgattatt gaagctcgca agggtaatac tcagccagct ttgtcatggt
      601 ttgcactaaa atcagcactc agcaataacc aaattgctga ctggttacag attgccttat
      661 gggccgggca agataaacag gttattaccg tttacaaccg ctaccgtcat cagcaattac
      721 cagcgcgtgg ttatgcagct gtcgccgtcg cttatcgtaa cctgcaacaa tggcaaaact
      781 cgcttacact gtggcaaaag gcgctctctc tggagccgca aaataaggat tatcaacggg
      841 gacaaatttt aaccctggca gatgctggtc actatgatac tgcgctggtt aaacttaagc
      901 agcttaactc tggagcaccg gacaaagcca atttactcgc agaagcctat atctataaac
      961 tggcggggcg tcatcaggat gaattacggg cgatgacaga gtcattacct gaaaatgcat
     1021 ctacgcaaca atatcccaca gaatacgtgc aggcattacg taataatcaa cttgctgccg
     1081 cgattgacga tgccaattta acgccagata ttcgcgctga tattcatgcc gaactagtca
     1141 gactgtcgtt tatgcctacg cgcagtgaaa gtgaacgtta tgccattgcc gatcgcgccc
     1201 tcgcccaata cgctgcatta gaaattctgt ggcacgataa cccagaccgt actgcccagt
     1261 accagcgtat tcaggttgat catcttggcg cgttattaac tcgcgatcgt tataaagacg
     1321 ttatttctca ctatcagcga ttaaaaaaga cggggcaaat tattccgccc tgggggcaat
     1381 attgggttgc atcggcttat ctcaaagatc atcagccgaa aaaagcacag tcaataatga
     1441 ccgagctctt ttatcacaag gagaccattg ccccggattt atccgatgaa gaacttgcgg
     1501 atctctttta cagccacctg gagagtgaaa attatccggg cgcgctaact gtcacccaac
     1561 ataccattaa tacttcgccg cctttccttc ggttaatggg cacgcctacg agcatcccga
     1621 atgatacctg gttacagggg cattcgtttc tctcaaccgt agcaaaatat agtaatgatc
     1681 ttcctcaggc tgaaatgaca gccagagagc ttgcttataa cgcaccagga aatcagggac
     1741 tgcgcattga ttacgcgagt gtgttacaag cccgcggttg gcctcgtgca gcagaaaatg
     1801 aattaaaaaa agcagaagtg atcgagccac gtaatattaa tctggaggtt gaacaagcct
     1861 ggacagcatt aacgttacaa gaatggcagc aggcagctgt cttaacgcac gatgttgtcg
     1921 aacgtgaacc gcaagatccc ggcgttgtac gattaaaacg tgcggttgat gtacataatc
     1981 ttgcagagct tcgtatcgct ggctcaacag gaattgatgc cgaaggcccg gatagtggta
     2041 aacatgatgt cgacttaacc accatcgttt attcaccacc gctgaaggat aactggcgcg
     2101 gttttgctgg attcggttat gccgatggac aatttagcga aggaaaaggg attgttcgcg
     2161 actggcttgc gggtgttgag tggcggtcac gtaatatctg gctcgaggca gagtacgctg
     2221 aacgcgtttt caatcatgag cataaacccg gcgcgcgcct gtctggctgg tatgatttta
     2281 atgataactg gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt ccattacggg
     2341 caatgaaaaa tggtgttaca ggcaacagtg ctcaggctta tgttcgctgg tatcaaaatg
     2401 agcggcgtaa gtacggtgtc tcctgggctt tcactgattt ttccgacagt aaccagcgtc
     2461 atgaagtctc acttgagggt caggaacgca tctggtcttc accatatttg attgtcgatt
     2521 tcctacccag tctgtattac gaacaaaata cagaacacga taccccatac tacaacccta
     2581 taaaaacgtt cgatattgtt ccggcatttg aggcaagcca tttgttatgg cgaagctatg
     2641 aaaatagctg ggagcaaata ttcagcgcag gtgttggtgc ctcctggcaa aaacattatg
     2701 gcacggatgt cgtcacccaa ctcggctacg ggcaacgcat tagctggaat gacgtgattg
     2761 atgctggcgc aacgctacgc tgggaaaaac gaccttatga cggtgacaga gaacacaact
     2821 tatacgttga attcgatatg acattcagat tttaaggata aatatgttac gtaatggaaa
     2881 taaatatctc ctgatgctgg tgagtataat tatgctcacc gcgtgcatta gccagtcaag
     2941 aacatcattt ataccgccac aggatcgcga atctttactc gccgagcaac cgtggccgca
     3001 taatggtttt gtagcgattt catggcataa cgttgaagac gaagctgccg accagcgttt
     3061 tatgtcagtg cggacatcag cactgcgtga acaatttgcc tggctgcgcg agaacggtta
     3121 tcaaccggtc agtattgctc aaattcgtga agcacatcga ggaggaaaac cgctaccgga
     3181 aaaagctgta gtgctgactt ttgatgacgg ctaccagagt ttttataccc gcgtcttccc
     3241 aattcttcag gccttccagt ggcctgctgt atgggccccc gtcggcagtt gggtcgatac
     3301 gccagcggat aaacaagtaa aatttggcga tgagttggtc gatcgagaat attttgccac
     3361 gtggcaacaa gtgcgagaag ttgcgcgttc ccgactcgtt gagctcgctt ctcatacatg
     3421 gaattctcac tacggtattc aggctaatgc caccggcagc ttattgcctg tatatgtaaa
     3481 tcgtgcatat tttactgacc acgcacggta tgaaaccgca gcagaatacc gggaaagaat
     3541 tcgtctggat gctgtaaaaa tgacggaata cctgcgtaca aaggttgagg taaatccaca
     3601 cgtttttgtt tggccttatg gcgaagcgaa tggcatagcg atagaggaat taaaaaaact
     3661 cggttatgac atgttcttca cccttgaatc aggtttggca aatgcgtcgc aattggattc
     3721 cattccgcgg gtattaatcg ccaataatcc ctcattaaaa gagtttgccc agcaaattat
     3781 taccgtacag gaaaaatcac cacaacggat aatgcatatc gatcttgatt acgtttatga
     3841 cgaaaacctc cagcaaatgg atcgcaatat tgatgtgcta attcagcggg tgaaagatat
     3901 gcaaatatca accgtgtatt tgcaggcatt tgctgatccc gatggtgatg ggctggtcaa
     3961 agaggtctgg tttccaaatc gtttgctacc aatgaaagca gatattttta gtcgggttgc
     4021 ctggcaatta cgtacccgct caggtgtaaa catctatgcg tggatgccgg tattaagctg
     4081 ggatttagat cccacattaa cgcgagtaaa atacttacca acaggggaga aaaaagcaca
     4141 aattcatcct gaacaatatc accgtctctc tcctttcgat gacagagtca gagcacaagt
     4201 tggcatgtta tatgaagatc ttgccggaca tgctgctttt gatggcatat tgttccacga
     4261 tgatgctttg ctttcagatt atgaagatgc cagtgcaccg gctatcacgg cttatcagca
     4321 agcaggcttt agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat ttaaacagtg
     4381 ggcccgcttt aaaagtcgtg cgttaactga cttcacttta gaacttagtg cgcgcgtaaa
     4441 agccattcgc ggtccacata ttaaaactgc acgaaatatt tttgcacttc cggtaataca
     4501 acctgaaagt gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa gctatgactg
     4561 gaccgctatt atggctatgc cttatctgga aggtgtcgca gaaaaatcgg ctgaccaatg
     4621 gttaatacaa ttgaccaatc aaattaaaaa catccctcag gctaaagaca aatctatttt
     4681 agaattacag gcacaaaact ggcagaaaaa tggtcagcat caggctattt cttcgcaaca
     4741 actcgctcac tggatgagcc tattacaact gaatggagtg aaaaactatg gttattatcc
     4801 cgacaatttt ctgcataacc aacctgaaat agaccttatt cgtcctgagt tttcaacagc
     4861 ctggtatccg aaaaatgatt aatcgcatcg tatcgttttt tatattatgt ctggtgttat
     4921 gcatacccct atgcgtagcg tactttcact ctggtgaact gatgatgagg ttcgttttct
     4981 tctggccgtt ttttatgtcc attatgtgga ttgttggcgg cgtctatttc tgggtctatc
     5041 gtgaacgcca ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa gataatccgt
     5101 ctatctccat tatcattccc tgttttaatg aggagaaaaa cgttgaggaa accatacacg
     5161 ccgctttagc acagcgttat gagaacattg aagttattgc cgtaaatgac ggttcaacag
     5221 ataaaacccg tgccatcctg gatcgcatgg ctgcacaaat tccccatttg cgggtcattc
     5281 atctggcgca aaaccagggg aaagccattg cgcttaaaac cggagctgcc gcggcgaaaa
     5341 gtgaatatct ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg gcggcatata
     5401 ttgtggaacc gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat cctcgtattc
     5461 gaacacgttc taccctggtg ggtaaaattc aggttggcga gtattcctca attattggtt
     5521 tgatcaagcg aacccagcgt atctatggaa acgtatttac cgtttccggt gttattgccg
     5581 catttcgtcg cagcgccctg gcagaagtgg gttactggag tgacgatatg atcaccgaag
     5641 atattgatat tagctggaag ctgcagttga atcagtggac gattttttac gagccacggg
     5701 cgctgtgctg gatattaatg cctgaaacgt taaaagggct gtggaaacag cgcctgcgct
     5761 gggctcaggg cggtgcagaa gtattcctca aaaatatgac aaggttgtgg cgcaaagaaa
     5821 actttcgaat gtggccgctg ttttttgaat actgcctgac gacaatatgg gccttcacct
     5881 gcctggtcgg tttcattatt tacgcagtcc aacttgccgg tgtaccgtta aatattgaat
     5941 tgacacatat cgctgcgaca catactgccg gaatattatt gtgtacgtta tgtttactgc
     6001 aatttattgt cagcctgatg atcgagaatc gctatgagca taatctgact tcatcgcttt
     6061 tctggattat ttggttcccg gttattttct ggatgctgag cctggcaacg acattggtat
     6121 catttacacg agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta agtcccgatc
     6181 gcgggattct gagaggttaa tatgaacaat ttaattatta cgacccgaca atcaccagta
     6241 cgtttactgg ttgattatgt tgccacaacc atcttgtgga cattatttgc gttgttcata
     6301 ttcttattcg ccatggatct gctgacgggt tattactggc aaagcgaggc cagaagccga
     6361 cttcagttct attttttgct ggcagtggcg aatgccgtcg tgttaattgt ctgggcgctg
     6421 tacaataagc tgcgttttca aaaacagcag catcatgcag cctaccaata tacgccgcaa
     6481 gaatatgcag agagcttagc aatacctgat gagctctatc agcaactaca aaaaagccac
     6541 aggatgagcg tacacttcac cagccagggg caaataaaaa tggttgtttc agaaaaagcg
     6601 ctagtccggg cataaacacc caaaacaaag cccggttcgc ccgggctctg caccgataat
     6661 acacttaact gtatgcatgc agcgtacgtt ggcaaagtgc cgaacgtacg cagtcctctt
     6721 taccgaaccg gacgatccca accatttcat cttcttcgaa acgttccagc gcatcactta
     6781 atccggagca cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg acgataaccg
     6841 tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc acattctgcg
     6901 cctcgtcaag aatgacgact gcattttcaa aggtacgtct acgcatatag gcgaacggcg
     6961 caatttccac cttccctatt tccggtcgca ggcagtactg cataaaggaa gcccctaagc
     7021 gccggaccag cacgtcgtag accgggcgaa aatagggagc aaacttttct gcgatatctc
     7081 caggtaagaa gccaagatct tcatcggctt gcagaactgg acgggtgacg ataatcctgt
     7141 cgacatcctt atgtatcagg gcctctgccg cttttgctgc gctgatccag gtttttccgc
     7201 acccggcttc gcccgtggcg aatatcaact gcttactctc aatagccttc agatagtgca
     7261 attgcgcttc atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg gccataccaa
     7321 tggcttctac gccgcccatc tgcacaagcg aggtgaccta ttcttcttca cgctgcttat
     7381 ggctgcgcga atcccgtctc agcacacgtt ttgcctcacg acgagctttg atcactgctt
     7441 tttgtcttcc catggagagc accttgagtt gtttgtattc atcacacgcg ccgttggcag
     7501 cgcgattatg cgcacgaaca tcagagggtt ggcttccttg taagccatag tttgcttttg
     7561 gataaaatgc cgaaaaacgg ctacgcacac cgtttgcggc gtcggtaaca cgagtaaaga
     7621 gaggaagtga gaaaaataca gagtgacgaa gttgctaccg gaggagaaat ctccgcaagt
     7681 ggtggagcgt agcttaacaa gacattgtcc agaacaggac gttaccatcc gcgatctcca
     7741 tagtgactga ctatcactgc cgggaacttc cgctgctact taataattac aacagatctc
     7801 gcattcattg caacaatata ttcacttata tttaaccata aaacaccatt tcagtgacat
     7861 tagtttctac tggaaagatg acagagtgat gacagatatg aaaaaagctg tgtgctttca
     7921 gcaggattcg catcaggcat ctgcgcacga acgccggatg cggcgtgaac gccttatccg
     7981 cctaccgaac ccgcaccgat tgtaggcata agaagacgcg caagcatcgc atcaggcatc
     8041 tgcgcaagaa cgccttatcc gtctataaac attaaacccg caataacgcg cttccgagat
     8101 aatcgttcgc gtccttcact cccggcagcg caaaaaaata accgccgccg ataggtttaa
     8161 cgtattcctc cagcgcttcg ccattgagcc ttttttgtac agtcaggaag cctttttcca
     8221 gatcgtgttg gtaacagaca aacagtagcc ccatatccag ttgcccggag ttggtgacgc
     8281 ccagtgaata actgtagcca cgacgcagca tcaggctgga ctcactctcc gccgtgcggg
     8341 gattcgccag ccggatatgg ctgtccagcg caatcacctt cccttccgga tcgctggcgt
     8401 aatcgggaac atcatgctca tgctgcattc ccagcggcgc accggtttgc ttatcacgac
     8461 caaaaatcgt ctgctgttct ttcagcggag ttctgtccca aaattccact cgaaactgaa
     8521 tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc tggctcctgc tgatctgccg
     8581 ttacccacac cactttttgc atcaacttat cattctggct atcgggattg gcagtgccgt
     8641 ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgcc gcgtgatcgg
     8701 aaataaaccc ttcccgcttc cagcgcacgc tgagcaaatc tggcgtatgt ttgatgatat
     8761 cgcgcagcgc atggatcacc gtgtcctggg tgttggcgca aatttgtagc aacacatcac
     8821 catgacataa cgccgcatcc agcgagtcgt tgggaaaacg cgtcatcttc tgcaactttt
     8881 tcggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc accgataacg
     8941 tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg agtggtggca
     9001 ggcgcggatt tggcgtttct ggtgccgctc cgccctgagt cagaaaagca aaacgctgag
     9061 tcaacaagcg aaacaaccgt tcaagatcgg gtttatcgct ggcaagcaca tcaaacgcca
     9121 ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca ccataaaacg
     9181 gctgtttctc attgcgtgca tccggtgaaa gcgtacccgg cgcactttgc gttttttgtg
     9241 catgagcgac cggacaactt cccgccagcg ccagcgcacc aatgcctttc agtaaacgtc
     9301 ggcgtgacgg ttcattcacg ccgtttttat cttcatactg cataatgctt aatccaatcc
     9361 cagcacaccg cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc ctttcagtgc
     9421 attccggtcg gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag tacggtattt
     9481 cgccagaatg gtatcgacct ttttaaagtt ggcatcgact tttgccaaca gttccgggtt
     9541 ggctttttgc agttgtggac gcagcagatc gacaattttc tgcgagcctt caacgttagc
     9601 ctggaaatcc cacagatcgg tgtggctgta gcgatcttct tcaccgctga ttttgctggc
     9661 tgccacttcc tcaatcagtc cggctgcacc gccgaccact tttgaaggtg ggaaagccag
     9721 ttcactgatg cgtttttgca aatcgaccac atcggtataa agctggtcag cgtactgatc
     9781 catgcctttg gtggtgttgt cgccaaacaa tgctttttcc agacggtgga aaccggtgaa
     9841 tttcggatcg gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc tgccatccag
     9901 atcggagaac agttcagcaa tcggttcgat gcgctcatag tgctggcgcg tcggcgcata
     9961 cagtgctttc gctttttcga tatcgcctgc tttaatagcg tcagtaaagg ctttcgtgtc
    10021 ggtcaccagc tgcgtggttt ccgccatgac atacgcttta tatgcagtaa ttgcaccacc
    10081 aagacttaac agcgcatcac tttgcgccgc atccgccgtt gcctcacctt tgacgatcaa
    10141 cttccctttc gggttagtca gcagaccgca ggtcatatcg tattcgccag gctgtaaatt
    10201 cgccgtcatt ttctggctaa agccaggggc gatattttcc cgctcttcca ccaccatcac
    10261 gcctttgagg atctcccact ccagcgcctt ctggctgtgg ttctgaataa tgaactgtgt
    10321 tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc ttatcggtca cggtcacctt
    10381 gacctgcggc acattagcgg cgttagccat aaaagcagaa gagaacagcg cagccacgct
    10441 caactgcaat gcgttacggc ggaagttaat ggtcatgacg agactatccc tttaaagtat
    10501 gttgtaacta agcaagagtt tgcgtcgtat ttgctacgcg gagcgagacg ctgtcgcccc
    10561 tgcgcgtggt ggcagggcaa atgccaccag cgccgggatg agataaataa accagacggc
    10621 gacttcgctg acgctcggtg cttcctgata gccaaaaatc ccttccatca gcgtgccaaa
    10681 cagcgagtga gttgagagga ccgcgctcat atcgaaggcg atttcctgaa agtggttcca
    10741 caatccggct tcatgaaatg cgcgaatggc accagccgcc agccctgcgg cgacgaagag
    10801 aataaacagg ctggtccatt taaaaaatgc accaagattg aggcgaatac cgccccagta
    10861 gagcaggaag ccgagcacca cggcagtagc aagaccgagc attgcaccca gcggcggcca
    10921 gatcccgaca tcttgttgaa atgccgccag caggaaaaag accgactcca gcccttccct
    10981 tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc cacgctgcaa
    11041 tgcgctatcg actgcctgtt ccagttgcac tttgacgttg cgcgacactt tgcgcatcca
    11101 gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt caaacagttc
    11161 ctgttctttt tgcggaaatt cgccggtggt ttcgttaatg aagataccca ggcccaggca
    11221 caacgcagcg gcaagcaaca cgccaatcca catcacacca atccatcggc ctcgctgggt
    11281 acgcttaagg tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc cttcgtgcaa
    11341 cataatgaga aacggaacaa acatgccacc cacccttaaa cgtttttctg agtcaatcta
    11401 tgcaaagaaa agtaaatcgc agtgatagcg attatcatta cggtaaagaa aaatacaagc
    11461 ggaatgttta taaaatgatg ttggaatgta aacaaagaga ggtaatgcca agggatataa
    11521 acgattattc cagggtagaa agatgggatt gtttgcaaag aaccatgtgt tcttgccgga
    11581 tgccgcgtaa acgccgtatc cagccgacaa aactgtccag actcaatgca tcagagcttc
    11641 catgtaaaac tgataagcgg gtgtatcaag caatttcgtc catccgcgat tatcaaatct
    11701 aactttttgc ccggaacata ataacagctc gggtgttggt ggcgtcaaaa tcgctgaagc
    11761 gttccccgaa ggccgggaca gcagggggcg gcggcgattg gccgccccct gcgcgctccc
    11821 tgcaccattc aatttatatg gcgaggcaca ttaagggagc gcaacccgct gctgaagcca
    11881 aacaaaaccg ccgcaggcta agtcccttag ctttcctgca accgtgacgg cggagccgaa
    11941 tgatagtgcg catcggcctc ggcaaagcgt ttttgcatcg ccgctgacgg cgctttaccc
    12001 agcaaactaa acactacaat cccaatactg ccgaagataa agcccggaat aatttcgtag
    12061 agtcccagcc agccgaactg cttccagacg ataaccgtca gcgcaccgat gatcatcccc
    12121 gccagcgcac cgttgcgcgt catgcgtgac cacatcaccg agaacagcac caccggacca
    12181 aacgccgcgc caaagcctgc ccacgcgtag ctcactaagc ccagcacgcg gttttccggg
    12241 tttgccgcca gcgcaatcgc caccagcgcc accaccagca ccatcacacg ccctacccac
    12301 accagctctt tctggctggc atgtttacgc agaaacgctt tgtataaatc ttcggtaatc
    12361 gcactggagc acaccagcag ctggcagctt aacgttgaca ttaccgccgc cagaattgcc
    12421 gacagcagaa tcccggcaat ccacgggtta aacagaattt gcgccagttc gataaacaca
    12481 cgttcagcgt tctggtttac cgcacctgct accgccgggt gctcattaaa gtaagcaatc
    12541 ccaaagaagc cgacagccac cgcccctgcc aggcagagga tcatccaggt catactgata
    12601 cgacgcgcat ggacaatgct gtggtgagaa tccgccgcca taaaacgcgc cagaatatga
    12661 ggctgaccga agtaacccag cccccaaccc atcaatgaga taatggcaac aaagttcagc
    12721 cctttgagca tatccacgtt ttcgatgctc ttttgtttga tcacttccag cgagtcacca
    12781 aagccaccga cactgataat gacgataacc ggtgtcagga tcagggcaaa aatcatcagg
    12841 ctggcctgta cagtatcagt ccagctcacc gcgaggaaac cgccaataaa ggtgtaaagg
    12901 atcgtcgccg cagctcccgc ccacagagcc gtttcgtagc tcatgccaaa ggtactttca
    12961 aacagacgcg cgcctgccac aatgcccgaa gcgcaataaa tggtgaagaa cagcaaaata
    13021 accagcgcgg agataatgcg caaaatgcgg cttttatctt caaagcgccc ggtgaaataa
    13081 tccggcaggg ttaaggcgtt attgttgtat tcggtatgca cacgcaaccg cccggccacc
    13141 agcttccagt tgatccacgc gcctaatgtc aggccaatgg cgatccagct ttcggaaatc
    13201 ccggaaagaa aaaccgcgcc cggcaacccc attaacagcc agccgctcat atccgacgca
    13261 cccgccgata atgccgtcac gaatggccca agactacgac cgcccagaat atagtcgtca
    13321 aagtttttcg ttgatcgcca ggcgataaac ccaatcaata tcatgccaaa gatatagaca
    13381 caaaatgtca ccaacatcgg tgtgctaata gccatctaaa gtctccaaaa aattattatc
    13441 ggcaatgtcg aaacttgccg ttatatctgc caccggaacg gggtaacaga gtttatgttt
    13501 taccagggcg accgtatcct gccggaagcg ctggttattc acaatcgatt taacacacca
    13561 tttacattaa attttagtgc tcagcgacac tatttttcat caggttgcac tctctcacat
    13621 tttttgcggt tgcacctttc aaaaatgtta actgccgcag agaaaaagtc tgatttattt
    13681 tttaatccct gtcatatcga tttcttttat taacatttca ttcattttta agcttgctac
    13741 gcaggtcaca tttaacatgg ttgcacaaag ttgcaacatc atggatattt cacgataacg
    13801 ttaagttgca cctttcagaa caacaggagt aatggcatgg gaaccaccac catgggggtt
    13861 aagctggacg acgcgacgcg tgagcgtatt aagtctgccg cgacacgtat cgatcgcaca
    13921 ccacactggt taattaagca ggcgattttt tcttatctcg aacaactgga aaacagcgat
    13981 actctgccag agctacctgc gctgctttct ggcgctgcca atgagagcga tgaagcaccg
    14041 actccggcag aggaaccaca ccagccattc ctcgactttg ccgagcaaat tttgccccag
    14101 tcggtttccc gcgccgcgat caccgcggcc tatcgtcgcc cggaaaccga agcggtttct
    14161 atgctgctgg aacaagcccg cctgccgcag ccagttgctg cacaggcgca caaactggcg
    14221 tatcaactgg ccgataaact gcgtaatcaa aaaaatgcca gtggtcgcgc aggtatggtc
    14281 caggggttat tgcaggagtt ttcgctgtca tcgcaggaag gcgtggcgct gatgtgtctg
    14341 gcagaagcgt tgttgcgtat tcccgacaaa gccacccgcg acgcgttaat tcgcgacaaa
    14401 atcagcaacg gtaactggca gtcacacatt ggtcgtagcc cgtcactgtt tgttaatgcc
    14461 gccacctggg ggctgctgtt tactggcaaa ctggtttcca cccataacga agccatcctc
    14521 tcccgctcgc tgaaccgcat tatcggtaaa agcggtgaac cgctgatccg caaaggtgtg
    14581 gatatggcga tgcgcctgat gggcgagcag ttcgtcacgg gtgaaaccat cgcggaagcg
    14641 ttagccaatg cccgcaagct ggaagagaaa ggtttccgtt actcttacga tatgctgggc
    14701 gaagctgcac tgactgccgc agacgcacag gcgtatatgg tttcctatca gcaggcgatt
    14761 cacgccatcg gtaaagcgtc taacggtcgt ggcatctatg aagggccggg catttcaatc
    14821 aaactgtcgg cgctgcatcc gcgttatagc cgcgcccagt atgaccgggt aatggaagaa
    14881 ctttacccgc gtctgaaatc actcaccctg ctggcgcgtc agtatgatat tggtattaac
    14941 atcgatgccg aagaggccga tcgtctggag atctccctcg atctgctgga aaaactctgt
    15001 ttcgagccgg aactggcagg ctggaacggc atcggttttg ttattcaggc ttatcaaaaa
    15061 cgctgcccgt tggtgattga ttacctgatt gatctcgcca cccgtagccg tcgccgtctg
    15121 atgattcgtc tggtgaaagg cgcgtactgg gatagtgaaa ttaagcgtgc gcagatggac
    15181 ggccttgaag gttatccggt ttatacccgc aaggtgtata ccgacgtttc ttatctcgcc
    15241 tgtgcgaaaa aactgctggc ggtgccgaat ctaatctacc cgcagttcgc gacgcacaac
    15301 gcccatacgc tggcagcgat ttatcaactg gcaggtcaga actactatcc gggtcagtac
    15361 gagttccagt gcctgcatgg tatgggcgag ccactgtacg agcaggtcac cgggaaagtt
    15421 gccgacggta aacttaaccg accgtgtcgt atttatgccc cggttggcac acatgaaacg
    15481 ctgctggcgt atctggcgcg tcgcctgctg gaaaacggtg ctaacacctc gtttgttaac
    15541 cgtattgccg acacctcttt gccactggat gaactggtcg ccgatccggt cactgctgta
    15601 gaaaaactgg cgcaacagga agggcaaact ggattaccgc atccgaaaat tcccctgccg
    15661 cgcgatcttt acggtcacgg gcgcgacaac tcggcagggc tggatctcgc taacgaacac
    15721 cggctggcct cgctctcttc tgccctgctc aatagtgcac tgcaaaaatg gcaggccttg
    15781 ccaatgctgg aacaaccggt agcggcaggt gagatgtcgc ccgttattaa ccctgcggaa
    15841 ccgaaagata ttgtgggctt tgtgcgtgaa gccacgccgc gtgaggtaga acaggcgctg
    15901 gaaagtgcgg ttaataacgc gccaatctgg tttgccactc ctccggttga acgcgcggcg
    15961 attttgcatc gcgctgccgt gctaatggaa agccagatgc agcaactgat tggtattctg
    16021 gtgcgtgaag ccggaaaaac cttcagtaac gccattgccg aagtgcgcga agcggtcgat
    16081 tttctccact actatgccgg acaggtgcgg gatgatttcg ctaacgaaac ccaccgtcca
    16141 ttagggcctg tggtgtgtat cagtccgtgg aacttcccgc tggctatttt caccgggcag
    16201 attgctgccg cactggcggc aggtaacagc gtgctggcaa aaccggcaga acaaacgccg
    16261 ctgattgccg cgcaagggat cgccattttg ctggaagcgg gtgtaccgcc aggcgtggtg
    16321 cagttgctgc caggtcaggg ggaaaccgtg ggcgcgcaac tgacgggtga tgatcgcgta
    16381 cgcggggtga tgtttaccgg ttcaaccgaa gtcgctacgt tactgcagcg caatatcgcc
    16441 agccgcctgg acgctcaggg tcgccctatt ccgctcatcg ctgaaaccgg cggcatgaac
    16501 gcgatgattg tcgattcttc agcactgacc gaacaggtcg tcgtggatgt actggcctcg
    16561 gcgttcgaca gtgcgggtca gcgttgttcg gcgctgcgcg tgctgtgcct gcaagatgag
    16621 attgccgacc acacgttgaa aatgctgcgc ggcgcaatgg ccgaatgccg gatgggtaat
    16681 ccgggtcgcc tgaccaccga tatcggtccg gtgattgata gcgaagcgaa agccaatatt
    16741 gagcgccata ttcagaccat gcgtagcaaa ggccgtccgg tgttccaggc ggtgcgggaa
    16801 aacagcgaag atgcccgtga atggcaaagc ggcacctttg tcgccccgac gctgatcgaa
    16861 ctggatgact ttgccgaatt gcaaaaagag gtctttggtc cggtgctgca tgtggtgcgt
    16921 tacaaccgta accagctacc agagctgatc gagcagatta acgcttccgg ctatggtctg
    16981 acgcttggtg tccatacgcg tattgatgaa accatcgccc aggtcactgg ctcggcccat
    17041 gttggtaacc tgtatgttaa ccgtaatatg gtgggcgcag tggtgggtgt gcagccattc
    17101 ggcggcgaag ggttgtccgg taccgggccg aaagcaggcg gtccgctcta tctctaccgt
    17161 ctgctggcga atcgcccgga aagtgcgctg gcagtgacgc tcgcgcgtca ggatgcagag
    17221 tatccggtcg atgcacagtt gaaagccgca ttgactcagc cgctaaatgc actgcgggaa
    17281 tgggcagcaa atcgtccaga attgcaggcg ttatgtacgc aatatggcga gctggcgcag
    17341 gcaggaacac aacgattgct gccggggccg acgggtgaac gcaacacctg gacgctgctg
    17401 ccgcgtgagc gcgtgttgtg tattgccgat gatgagcagg atgcgctgac tcagctcgcc
    17461 gccgtgctgg cggtgggcag ccaggtactg tggccggatg acgcgctgca tcgtcagcta
    17521 gtaaaggcat tgccatcggc agtcagcgaa cgtattcaac tggcgaaagc ggaaaatata
    17581 accgctcaac cctttgatgc ggtgatcttc cacggtgatt cggatcagct tcgcgcattg
    17641 tgtgaagccg ttgctgcgcg ggatggcgca attgtttcgg tgcagggttt tgcccgtggc
    17701 gaaagcaata tccttctgga acggctgtat atcgaacgtt cgctgagtgt gaataccgct
    17761 gccgctggcg gtaacgccag tttaatgact ataggttaaa caggtagccg gaggatgtta
    17821 caacctcctc cggcatcttt aacgtggtcg aatcccctca ataataatcc gctgcacgtt
    17881 ttcaaccgtt tgattgaaaa atatctcatc gcgcaacgtc gcgcctgtca ccgcctccac
    17941 ctgaggggcg aaatcggcgt aatgttgagt ggaagcccaa atcatgaaaa tcaaatgctg
    18001 cggatcaatc ggcgcgagtt tgccgctttt gacccaaccg gcaatcagcg ccgatttctc
    18061 atcaattaat gccttcaaat cgcccgtcag ttcatccatt aacagcggcg cgcctgccag
    18121 catctccata cagaacaggc gcgaagcctg cggataatcg cgggagactt ccagcttcag
    18181 acggatgtac tcttttatcg ccgccagcgg agcgaaatct tcacgaaacg cttttaacgg
    18241 tgccagccag atatcgagaa tctgccgcag cacggcaata tacagcgcct cttttgacgg
    18301 aaagtaatac agcagattgg tttttgaaac acccgccaac tctgcgatct gctccagcct
    18361 tgtgccgtga aaaccgaatt gtgaaaaagt gtccagtgct gcgctaagaa tcgctttttt
    18421 cttcgcgctt actgcgcgcg aacgtttacc cgttgttttc actgcgcctt gcgtcatgcg
    18481 ctctcccctc ttgtagattg gcaacaggat agcaaaaccc ccgcccggcc tcgaccttct
    18541 gcactctcat cgcgcacaga tgcatgtttt atgtgcaact gttttgaccg tttagtccac
    18601 tttttaccag atattttaga taacgcattg ttaacatatt aacaacatta actttttaaa
    18661 aactggcatc cgctttgcaa acaagccatt acgcggctga aaaggatatg caggatgcag
    18721 cgccccgtct gactttcact ttacgagacg aagagaggtt gatgatgaaa attggcgtat
    18781 tcgtacctat tggcaacaac ggctggctca tttcgaccca cgcgccgcag tacatgccga
    18841 cctttgaact gaataaagcc attgtgcaaa aagcggagca ctaccatttc gatttcgccc
    18901 tgtcgatgat caaactgcgt ggctttggcg gcaaaactga gttctgggat cacaaccttg
    18961 agtcgttcac cttgatggcg gggctggcgg ccgtcacctc gcgcattcag atttacgcca
    19021 ctgctgccac cttaacgtta cctccggcaa tcgtcgcccg tatggccgca accatcgact
    19081 ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg ctggcaaaag cccgagtatg
    19141 agcagatggg catctggcct ggcgatgact atttctcccg tcgttacgac tatctcaccg
    19201 aatatgttca ggtgctgcgc gacctgtggg gcacggggaa aagcgatttt aaaggcgatt
    19261 ttttcaccat gaatgattgt cgcgtcagtc cgcaaccgag tgtccccatg aaagtgatct
    19321 gcgccgggca aagcgacgct ggcatggcgt tctccgccca gtatgccgat ttcaacttct
    19381 gtttcggcaa aggcgtaaat acgcccacgg ctttcgcccc gaccgctgcg cggatgaaac
    19441 aggccgcaga gcaaaccggg cgcgacgttg gttcttatgt gttgtttatg gtgattgccg
    19501 atgaaaccga cgatgccgct cgcgccaaat gggaacacta caaagcgggc gcggatgaag
    19561 aggcgttaag ctggctaacc gaacaaagtc agaaagatac ccgctccggt actgatacca
    19621 acgtccgtca gatggccgat cccacttcgg cggtaaacat caatatgggg acgttagtcg
    19681 gttcttacgc cagtgtcgcg cgcatgttag atgaagtcgc aagcgtgcct ggtgccgaag
    19741 gcgtgctgtt gaccttcgat gattttctgt cgggaatcga aaccttcggc gagcgcattc
    19801 aaccactgat gcagtgccgc gcccatctac ctgcgctgac tcaggaggtg gcatgatgac
    19861 aaccttaacc gctcgaccgg aagccattac cttcgatccg cagcaaagtg cgctgatcgt
    19921 ggtggatatg caaaacgcct atgccacgcc aggcggctac ttagatctcg ccgggtttga
    19981 tgtctcaacc actcgcccgg tcattgccaa cattcaaacc gctgtgaccg cagcgcgagc
    20041 ggcagggatg ttgatcatct ggtttcaaaa tggctgggat gaacagtatg tcgaggctgg
    20101 cggccccggc tcaccgaatt ttcataaatc gaacgccctg aaaaccatgc gtaatcagcc
    20161 gcagctgcag gggaaattgc tggcgaaagg ctcctgggat tatcaactgg tggatgaact
    20221 ggtgccgcag cctggcgata ttgtgctgcc gaagccgcgc tacagcggtt tcttcaatac
    20281 accgctggac agcattttgc gcagccgtgg aatacgccat ctggttttca ccggtatcgc
    20341 taccaacgtc tgcgtcgaat cgacgctacg cgatggcttt tttctggagt atttcggcgt
    20401 ggtgctggaa gacgcaacac accaggcggg gccggaattt gcacagaaag ccgcgttgtt
    20461 caatatcgaa accttttttg gctgggtcag cgacgtcgaa acgttctgcg acgcgctttc
    20521 tcccacatcc tttgctcgta tcgcttaagg agtttaacga tgccaaaatc cgtaattatt
    20581 cccgctggca gcagcgcacc gctggccccc ttcgttcccg gcacgctggc tgatggcgtg
    20641 gtgtatgtct ccggtacgct ggcttttgat caacataata acgtgctgtt tgccgatgac
    20701 ccaaaggcgc aaacccgcca cgttctggaa actatccgca aggtgatcga gacggcgggt
    20761 ggcacgatgg cggatgtgac cttcaacagc atctttatta ccgactggaa aaattacgcc
    20821 gcgattaacg aaatctacgc cgagtttttt ccgggtgata aaccggcgcg attctgcatt
    20881 cagtgcggac tggtaaaacc tgacgcgctg gtggaaatcg ccacaattgc gcatatcgcc
    20941 aagtgaggcc gcgatgaaac tttcactctc acctccccct tatgctgatg cgcccgtagt
    21001 ggtgttgatt tcgggtcttg gcggtagcgg cagttactgg ttacagcaac tggcggtgct
    21061 ggagcaggag tatcaggtag tctgttacga ccagcgcggc accggcaata atcccgacac
    21121 actggcagaa gattacagta tcgcccagat ggcagcggaa ctgcatcagg cgctggtagc
    21181 cgcagggatt gagcattacg cggtggtcgg ccatgcgctc ggtgcgctgg tgggaatgca
    21241 gctggcgctg gattatcccg cgtcggtaac tgtgctgatc agcgttaacg gctggctacg
    21301 aataaacgcc catacgcgcc gctgttttca ggttcgcgaa cgattactgt atagcggcgg
    21361 cgcgcaggca tgggtggaag cgcagccgtt gttcctctat cccgccgact ggatggcggc
    21421 ccgcgcacct cgccttgagg cagaagacgc gctggcactg gcgcattttc agggcaaaaa
    21481 taatttactg cgtcgactta acgccctcaa acgcgctgac tttagtcacc atgcggatcg
    21541 catccgctgc ccggtgcaaa tcatctgcgc cagtgatgat ctgctggtgc caacagcatg
    21601 ttccagtgaa cttcatgccg ccctgcccga tagccagaaa atggtgatgc cctatggcgg
    21661 acacgcctgc aacgtgaccg atcccgaaac gtttaatgct ctgttactca acgggcttgc
    21721 cagcctgtta catcaccgtg aagccgccct gtaaggaatt gctatgaacg aagccgttag
    21781 cccaggtgcg cttagcaccc tgttcaccga tgcccgcact cacaacggct ggcgggagac
    21841 acccgtcagc gatgagacgt tacgggagct ttatgccctg atgaaatggg ggccgacatc
    21901 agctaactgt tctccggcac ggatcgtgtt tatccgcact gcagaaggaa aagaacgtct
    21961 gcgcccggcg ctttccagcg gcaatctaca aaaaaccctg accgcgcccg tcaccgctat
    22021 cgtcgcctgg gacagtgaat tttatgaacg gttaccacta ctgtttcccc acagtgatgc
    22081 ccgcagttgg tttacctcca gcccacaact tgccgaagaa acagcgtttc gcaacagttc
    22141 catgcaggcg gcctatctga tcgtcgcttg ccgtgcgctg ggactggata ccggcccgat
    22201 gtcgggtttt gaccgtcaac acgttgacga cgcctttttt gcgggcagca cgctgaagag
    22261 caatctgctg attaatatcg gctatggcga tagcagcaaa ctttttgcgc gcctgccacg
    22321 tctgtccttt gaagaagcct gcgggctgtt gtaaggagca aactatgaac atagttgatc
    22381 aacaaacctt tcgcgatgcg atgtcctgta tgggcgcggc ggtcaatatc atcaccacgg
    22441 acggtccagc cgggcgcgcc gggttcaccg ccagcgccgt ctgcagcgtg accgatacgc
    22501 cgcccacatt actggtgtgc ctgaatcgtg gggcgtccgt ctggccggta ttcaatgaaa
    22561 atcgaacgct gtgtgtaaat acacttagcg ccgggcagga gccgctttcc aacctttttg
    22621 gcggcaaaac gcccatggaa caccgctttg ccgccgcccg ctggcagacg ggtgtgaccg
    22681 gatgcccgca actggaagag gccctggttt cgtttgactg ccgtatcagc caggtggtga
    22741 gcgtcggcac ccacgacatt ctgttttgcg ccatcgaagc gattcatcgt cacgccacac
    22801 cctacgggct ggtgtggttc gatcgcagtt atcacgcgct gatgcgccct gcttgttaac
    22861 ctccttaagg agacagctga tggcaatgtt cggttttcct cactggcagt taaaatcgac
    22921 ctctacagag agcggcgtgg tcgcgccgga tgaacggttg ccgtttgctc agacggcagt
    22981 aatgggcgtt caacacgcgg tggcgatgtt tggcgcgacg gtgttaatgc cgattctgat
    23041 ggggctggat cccaatcttt ccattttaat gtcggggatc ggcacgttac tgtttttctt
    23101 tattaccggc gggcgcgtcc ccagttatct cggctccagc gcggcttttg tcggcgtggt
    23161 gatcgccgcg acgggcttta acggtcaggg catgaacccg aatatcagca tcgcgctggg
    23221 cgggattatc gcctgtgggc tggtttatac cgtcattggc ctggtggtga tgaaaatcgg
    23281 tacacgctgg attgaacggc taatgccgcc ggtggtgacg ggtgcggtgg tgatggcgat
    23341 tggcctgaac ttagcgccta tcgcagtgaa aagtgtatcg gcttcggcct ttgatagctg
    23401 gatggcggta atgacggtgc tgtgtattgg gctggtggcg gtgtttaccc gaggcatgat
    23461 ccagcggtta ttgattctcg taggattaat tgtcgcctgt ctgctgtatg gcgtgatgac
    23521 caacgtttta gggctgggaa aagccgtcga ttttactctc gtcagccatg ctgcctggtt
    23581 cggtttgcca catttttcca ctcctgcttt taatggtcag gcaatgatgc tgattgcgcc
    23641 cgtggcggtg attctggtgg cggaaaactt aggtcatctc aaggcggtcg ccgggatgac
    23701 cggacgcaat atggacccgt atatggggcg agcattcgtc ggtgatggtc tggcaacgat
    23761 gctttccggc tctgtcggcg gtagcggcgt caccacctat gctgaaaata tcggtgtgat
    23821 ggccgtgaca aaagtctact cgacgctggt gtttgttgct gctgccgtca tcgccatgct
    23881 gttgggcttt tcgccgaaat ttggcgcgct gatccatacc attccggccg cggtgattgg
    23941 tggtgcatca attgttgtgt tcgggctgat tgccgtcgca ggcgcaagga tatgggtaca
    24001 aaaccgtgtc gatttaagcc agaacggtaa tttgattatg gtcgcagtaa ccttagtgct
    24061 gggcgcgggt gattttgctc tcacgctggg cggttttacg ttgggaggga ttggtacagc
    24121 aacctttggc gcgattttac tcaatgcgtt gttaagcaga aagttggttg acgttccacc
    24181 gccagaagtt gttcatcagg agccataaac agaaaaccgc cgggattacc ggcggtgatg
    24241 actttttgac gagcctaaga aaaaaatgca ttcgtcattg attagcccgg ctagttgtcg
    24301 cttttgccgt gactgctctt gccacctttt ttacctgctt cagatgcgcg ctgcgggtca
    24361 ttcttgaaat tacccccgct gtgctgtcca ccttttttac ctgcttctga tgctctttcg
    24421 cggtcttctg caaaattgcc ggaaccgcct cgatggtttg ccattactga cctccgtctg
    24481 ggtatagaca tcatattgca tagaattaac gcggcgtatg gcggctgcca tattttcgct
    24541 acgtgggact aagcttagtg aactgacgcg ggcagacccg cgtttggtaa tatcctgcaa
    24601 cagcttctta gcgagaatag atatcatctc aaataattat ccataagccg ctcttatgat
    24661 taaaaaaacg cgatgagata aaaatgtgtc attttgcgac aaaattacgt gcttgtgaaa
    24721 gttgttataa atcaaataag tggttgtgaa atttgcactc tgaaaaggac gtcttatctt
    24781 taaataagtg gtagcgaatc gctacggaat agagataaca cgaggagtgg ttagaaatgg
    24841 ctaaagttct ggtgctttat tattccatgt acggacatat tgaaacgatg gcacgcgcag
    24901 tcgctgaggg tgcaagcaaa gtcgatggcg cagaagttgt cgttaagcgt gtaccggaaa
    24961 ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac gcaaactgca ccggttgcaa
    25021 ccccgcaaga actggccgat tacgacgcca ttatttttgg tacacctacc cgctttggca
    25081 acatgtccgg tcaaatgcgt accttcctcg accagacggg cggcctgtgg gcttccggcg
    25141 cactatacgg aaaactggcg agcgtcttta gttccaccgg tactggcggc ggtcaggaac
    25201 aaactattac ttcaacctgg acgacccttg cgcatcacgg catggtaatt gtccccattg
    25261 gctacgcagc gcaggaatta tttgacgttt cacaggttcg cggcggtacg ccgtacggcg
    25321 caaccaccat cgcaggcggt gacggctcac gccagccaag ccaggaagaa ctgtctattg
    25381 ctcgttatca aggggaatat gtcgcaggtc tggcagttaa acttaacggc taatcttcaa
    25441 caggaggata cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg aatgggcatc
    25501 tctgcgcaat acgtcgccgg aaatagccga ggccattttc gaagtcgccg ggtacgacga
    25561 aaaaatggcg gaaaagattt gggaagaagg tagcgatgaa gtgttagtca aagcctttgc
    25621 taagaccgat aaagattcgc ttttttgggg cgaacagacc atcgaacgta aaaacgttta
    25681 accagcgact cccccgcttc tcgcggggga gttttctgtt atttcaccgc ttcattcaac
    25741 acgctatcaa atttatccat cgggcagaaa ccgttggcat ctatcgggca accgcttaat
    25801 tccagcgtca cacgctgagc aggtgcctgc agggttaacg catcggcatt acgtaactgt
    25861 tccgcactct gatacacata ttcaattttc atcaaatcgc gattggcttt gctgtcatgc
    25921 caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc atgcaactga
    25981 tacggtttga aatccagcgc cgttaacaga gaggcaatgt tggagtcgtg cccaaccaac
    26041 actgtaattt tcggtgcgct ggtgcgatcg gtgaccagag ctttgtcgat ataactgacc
    26101 agcggtttcg caacattgcg cgccacttcc ggtgaggtaa acaggctgtc ctggtagccg
    26161 tttttaagct tcgacaacac cttccactgc tggtcagatt tgatttctcc ccaggccacc
    26221 tgatccatcg gaaaaccttc gtaatattgc aaagtaaacg catctaccag cgagttgccg
    26281 actttcagcg gcccggaaac acctggttct tgttgatact tggcgctaaa ggtatttttg
    26341 ccatccacca gcgaacactg ttgtttctct ttacaggcag gggaatcttt atagttaacg
    26401 attttttcca gtagctggta gctgtcggta agctggagtt tgctgagctc tttctccatt
    26461 gccgccaccg cctgttcact gaatgcggcg gaatcatcgg tgatcaccgg gttaaaggtt
    26521 gggtccatag tgcccatttt ttcctggtga tgcacaggaa tatcacaccc cgggaatgcg
    26581 ccggtaataa agaactgtgc ggtcgcaacg gtacgttgca gactattggc ataggcataa
    26641 acggtgtacg gcggcgggca ttcccccgat ttcaccatcc cctgctctgc cagccattca
    26701 cgcatgtaat ggcccatata cacttcgagc acgccacctt tggtggtgag ttgcccaccg
    26761 gggacgtccc attctggcca tttattcggc gtcgactgct ccagcacact gccattgttc
    26821 gccagcggcg cacgtaagtt atggcggctc atcatgagca cttgctgtag ctgatagcct
    26881 tccggtacgg tttgtgcctg agcgtttgaa gcgagtaaaa ctatccctgc cacagctgcg
    26941 gcgattagcg ttttgttcat tcctggcacc tcttttgtta tcaatcacta aagagtgtga
    27001 cagaaatatg acttttttcc gggaacaggt tcgcaaaatg gcttgttgaa cgcctgtttt
    27061 tctatataca tcttcaaaaa caggcaaggt ttgacgcaaa ctatgcctga ttagcccctg
    27121 cctcgcgtac gccgctgata attgcacctg tggctaaaca gaatatctgc ggcgttacca
    27181 atacagagac aatatttacc aataaaaaag tgtaagacta ataacaatg
//
LOCUS       DAZFYC010000067        26787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFYC010000067 DAZFYC010000000
VERSION     DAZFYC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.067"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(106..1107)
                     /locus_tag="ACHKW5_003001"
     CDS             complement(106..1107)
                     /locus_tag="ACHKW5_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7512355.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
     gene            complement(1113..1460)
                     /locus_tag="ACHKW5_003002"
     CDS             complement(1113..1460)
                     /locus_tag="ACHKW5_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO7512356.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNSDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            complement(1490..2140)
                     /locus_tag="ACHKW5_003003"
     CDS             complement(1490..2140)
                     /locus_tag="ACHKW5_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512357.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFNL"
     gene            complement(2156..2560)
                     /locus_tag="ACHKW5_003004"
     CDS             complement(2156..2560)
                     /locus_tag="ACHKW5_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7512358.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(2650..2787)
                     /locus_tag="ACHKW5_003005"
     CDS             complement(2650..2787)
                     /locus_tag="ACHKW5_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512359.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            2859..3062
                     /locus_tag="ACHKW5_003006"
     CDS             2859..3062
                     /locus_tag="ACHKW5_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7512360.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            3084..3434
                     /locus_tag="ACHKW5_003007"
     CDS             3084..3434
                     /locus_tag="ACHKW5_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO7512361.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            3445..3723
                     /locus_tag="ACHKW5_003008"
     CDS             3445..3723
                     /locus_tag="ACHKW5_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512362.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHEIISEAEKARQVNGDKPYIEIICMDSEAEIQKAD"
     gene            3735..3977
                     /locus_tag="ACHKW5_003009"
     CDS             3735..3977
                     /locus_tag="ACHKW5_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO7512363.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGVHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            3974..4087
                     /locus_tag="ACHKW5_003010"
     CDS             3974..4087
                     /locus_tag="ACHKW5_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512364.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIINSVKKIERFFSDE"
     gene            4181..4591
                     /locus_tag="ACHKW5_003011"
     CDS             4181..4591
                     /locus_tag="ACHKW5_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512365.1"
                     /translation="MHAKEEGIIRALKEISKMESEVAKKAVANAHMDVATHTMIVAKV
                     TAEAAKIIEEQGVELALLKTKPVTGLDLSDTGRLIYTIGSEPQRYTIIAGLQNKYLIT
                     PHPIRESTLLTNLRLIERSQVAFIDDARHTVFNA"
     gene            4615..4818
                     /locus_tag="ACHKW5_003012"
     CDS             4615..4818
                     /locus_tag="ACHKW5_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512366.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            4815..5081
                     /locus_tag="ACHKW5_003013"
     CDS             4815..5081
                     /locus_tag="ACHKW5_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO7512367.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            5078..5377
                     /locus_tag="ACHKW5_003014"
     CDS             5078..5377
                     /locus_tag="ACHKW5_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512368.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            5700..5912
                     /locus_tag="ACHKW5_003015"
     CDS             5700..5912
                     /locus_tag="ACHKW5_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO7512369.1"
                     /translation="MANRKQQRAYAARRHIQTEINRRLFRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLRELQQLFQ"
     gene            6003..6392
                     /locus_tag="ACHKW5_003016"
     CDS             6003..6392
                     /locus_tag="ACHKW5_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO7512370.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQEWQRVTGYQP"
     gene            6389..9229
                     /locus_tag="ACHKW5_003017"
     CDS             6389..9229
                     /locus_tag="ACHKW5_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7512371.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     AREEQEAKEEAFFAQSAMGKKLMASLEEAGQREKLAQSIISKRRATEQDPVARAFATL
                     KALPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWVRSLKRHERRALMRS
                     LRDKQAKNSSDEMQSWTQSRKQQRPLPDNHELLAREWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPLASYLA
                     QLGASDPEAATRLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKA
                     DLLRGGLTSGNKKQTETGFTNPVNEQKTRRDI"
     gene            9306..10265
                     /locus_tag="ACHKW5_003018"
     CDS             9306..10265
                     /locus_tag="ACHKW5_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO7512372.1"
                     /translation="MKICIDDGSTNIKLAWTENSERRNAISPNSFKSEWSAPFGGSQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            10270..10581
                     /locus_tag="ACHKW5_003019"
     CDS             10270..10581
                     /locus_tag="ACHKW5_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7512373.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            10645..11015
                     /locus_tag="ACHKW5_003020"
                     /pseudo
     CDS             10645..11015
                     /locus_tag="ACHKW5_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(11118..11648)
                     /locus_tag="ACHKW5_003021"
     CDS             complement(11118..11648)
                     /locus_tag="ACHKW5_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512374.1"
                     /translation="MKKDSYPYLICMTVSVLGIIFLFYWWRADIYRITYLNSRISNYY
                     ILCSMGMSFFFSLFLVKRGVVKQSGWKGLSEYLKVYAGSCILTGIFLIIPLMMVTYFL
                     PGVTSSYVAPYEYTSGSSRSCSGAFVDDPELHKNILICYPYGNYEYDNIIYVEKKTNA
                     LGVVVTYAQTARDDAE"
     gene            complement(12179..13225)
                     /locus_tag="ACHKW5_003022"
     CDS             complement(12179..13225)
                     /locus_tag="ACHKW5_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7512375.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(13225..14976)
                     /locus_tag="ACHKW5_003023"
     CDS             complement(13225..14976)
                     /locus_tag="ACHKW5_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO7512376.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPKQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVFPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTRRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            15131..15967
                     /locus_tag="ACHKW5_003024"
     CDS             15131..15967
                     /locus_tag="ACHKW5_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7512377.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            15991..17043
                     /locus_tag="ACHKW5_003025"
     CDS             15991..17043
                     /locus_tag="ACHKW5_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7512378.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            17089..17889
                     /gene="gpM"
                     /locus_tag="ACHKW5_003026"
     CDS             17089..17889
                     /gene="gpM"
                     /locus_tag="ACHKW5_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO7512379.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSTFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASTPQTQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            17991..18485
                     /locus_tag="ACHKW5_003027"
     CDS             17991..18485
                     /locus_tag="ACHKW5_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7512380.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAI
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILRRPRIF
                     AELC"
     gene            18485..18685
                     /locus_tag="ACHKW5_003028"
     CDS             18485..18685
                     /locus_tag="ACHKW5_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO7512381.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            18688..19011
                     /locus_tag="ACHKW5_003029"
     CDS             18688..19011
                     /locus_tag="ACHKW5_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7512382.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            19008..19400
                     /locus_tag="ACHKW5_003030"
     CDS             19008..19400
                     /locus_tag="ACHKW5_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO7512383.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGVR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPQYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            19397..19804
                     /locus_tag="ACHKW5_003031"
     CDS             19397..19804
                     /locus_tag="ACHKW5_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO7512384.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASALKST
                     ADTLNIISAGVQDMQQVLAQLRAENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            19942..20409
                     /locus_tag="ACHKW5_003032"
     CDS             19942..20409
                     /locus_tag="ACHKW5_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7512385.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            20402..21037
                     /locus_tag="ACHKW5_003033"
     CDS             20402..21037
                     /locus_tag="ACHKW5_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO7512386.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            21049..21615
                     /locus_tag="ACHKW5_003034"
     CDS             21049..21615
                     /locus_tag="ACHKW5_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7512387.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVKLDTPVVECTNLLKTAEIDVTKGGKMSGNITHSGG
                     DFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            21612..21962
                     /locus_tag="ACHKW5_003035"
     CDS             21612..21962
                     /locus_tag="ACHKW5_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO7512388.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            21966..22862
                     /locus_tag="ACHKW5_003036"
     CDS             21966..22862
                     /locus_tag="ACHKW5_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7512389.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLSEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            22855..23385
                     /locus_tag="ACHKW5_003037"
     CDS             22855..23385
                     /locus_tag="ACHKW5_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO7512390.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            23388..24233
                     /locus_tag="ACHKW5_003038"
                     /pseudo
     CDS             23388..24233
                     /locus_tag="ACHKW5_003038"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            24753..25658
                     /locus_tag="ACHKW5_003039"
     CDS             24753..25658
                     /locus_tag="ACHKW5_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7512391.1"
                     /translation="MASKGQVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHLKGMT
                     AVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQ
                     DGIKSHTHSASVSSTDLGTKTTSSFDYGTKTTSLFDYGTKTTSNAGSHTHSIPTSDGA
                     NQWGKIPLTSGRLATQYTSATTGSSGSHSHTVGIGSHQHTVGIGAHTHSVAIGSHGHT
                     ITVNAAGNAENTVKNIAFNYIVRLA"
     gene            25658..26236
                     /locus_tag="ACHKW5_003040"
     CDS             25658..26236
                     /locus_tag="ACHKW5_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7512392.1"
                     /translation="MAFRMSKQSRTLKIYNLLAGTNEFIGDGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNTDEASWHLVEDHRGYTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTSWVKDAEAEKLFRSQEAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVDTTVAADIEWPVAPQ"
     gene            complement(26280..>26787)
                     /locus_tag="ACHKW5_003041"
     CDS             complement(26280..>26787)
                     /locus_tag="ACHKW5_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO7512393.1"
                     /translation="WKHEVIRRKRMDIWRLLREKKQRNRNFLFWWRLANEMYINGNKL
                     HKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHAGIVVHFAVDAGENLVLRQNTTI
                     GQIDGDIPGSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINKDIPSNCTYITK
                     KSGVVLYK"
BASE COUNT         7029 a         7186 c         6747 g         5825 t
ORIGIN      
        1 actatcaacc accatttata aatcataaca ttatgatttt attaactttg aaaatggctc
       61 tatacaacgc catttgaatc tattgtcact ttttatcgcc actttttacc ctctattgtc
      121 aaaagggttc aattcaacag cagcctctaa atgacctgga gcaaaatgcg cataacgcat
      181 agtcattttt atatcgctat gccccagtat tttttgcaac acaagaatat ttccgccccg
      241 catcataaaa tgactggcaa acgtgtgacg tagcacatga gttaattgcc catcaggaag
      301 ctcgatcttc gctctcttaa ttgcagcgtc aaaagcctca taacatggtg aaaatagcgc
      361 tcctcgtttt ttgggaagca tagcctgcaa ttgaggtgaa atcggtacag tgcggttctt
      421 ctttccttta gttttaacaa atgtgattcg accgggcagt acttgagatt gctttaatcc
      481 ttctgcttca ctccaccgag caccagtcgc aagcccaata cggacaacaa cccccaaatc
      541 tttattccgt gactcatcac acgcaatcag aaggcgttca atctcatcta catacagaaa
      601 cgccagttcc ttttcttcct cacgaaactt gcgaatacca gtcagggggt tttcaccaga
      661 ccactcccca aggcgcttca gttcggcaaa aacagcatgt agatatgact gctcgcgatt
      721 aacggttgct tcactaagtt ttttcttccc cttgggattc cattctcctg atagccttct
      781 ttcccgataa gtggcaaaca tatttttgtc aaactgagaa gcaaatggat ctcccagcct
      841 ggaacaaatc gcctcaagtt tgactttgcg ctctgcacca gaggacaagg ttttaccgta
      901 catctcaaac caacgagcaa tcaactcaga aagacgagga ccagaaccat cttgaaactc
      961 gtctccaact ctactattca ttaaacggcg ctcataagag agcgcctcac tttttgtcgc
     1021 aaactgttta cgaatgcgtt ttcccgatgc cccgtaggga taacattcgc aaagccattt
     1081 acctgatgga atcttacgaa ccgacatttt agttacttat cacataaatc aaatgcagcc
     1141 ttagtgacat cccccagact cttttttaac cctggggcgg catcattatc tagccaaaat
     1201 ggattattgt tatctaacgg taacgcacca aatgttttac cttttattcg agccaaacct
     1261 gtaagtgcat ataacttatt atcgtcaaaa ttcatcacat aaggattacc atcaagacac
     1321 tgtaattgaa cctcatcaga attaaatggc cataccccat tgaaactctc acgttcaata
     1381 gttttaaaag gcattgcgac ggcggaaaaa gaaaacatag ataaaaaagt aactaatagt
     1441 tgaacctttt ttactttcat atcattacct caatttagct caagtaagat tacaaattaa
     1501 aaaacgccct agaaatgaca ccgcctacca aaacccctac gcagataaag aatattattt
     1561 cttttggata aagtcggatt aattctgaag cacgaagtcg gacttctggt aaggtcgaac
     1621 tctctgtgtg gcttgatgcc gattgttgct ctaaccacga caatgcagac tgtaactgag
     1681 aacgagtaag atcgtttaaa cgtcctgtac cgaaattgat atggcaatac cgcagaagtt
     1741 tttgtcgaag tccacagtct tcactgttac gtagtaataa acttacaaga gccttacagg
     1801 catcatgatc tttacatcgc tcaagcattg catgcagaaa actctccgct gttttatatt
     1861 gatttactgt catatcatca ataccagcta caccaatctc cgcatgtact ttttgccaaa
     1921 taataaacgc ttcagtattg ctagcttctg caatagcagc aaccaagcta tttagctcct
     1981 tacgctgagc cttaagcaaa gggcgatcgt catcatcatt attcgaaggg attgcgatat
     2041 tgacagtatg agaaccatca tatcgctcta tctgaatatt cttttcgtga aaatcacgcc
     2101 cagcaacgcg attgtttgaa ccgtttgagt tgacggccat gtcacctccc tactatcacc
     2161 tacccttagt ttcgttatag tcacgaccag cgatacggtt attaccacca gaaatattta
     2221 actcacgtcc tgatggctga gtttcctttt cactgatcgc accttttaaa gccccaatca
     2281 ccgcgttttt cacatctaac gaagctgctc gaaagcgagt aatcaactcc tgctcatcat
     2341 cgttataagt ttcaggtgag tgaattccca acacaacata ctgaacatca aggccaaaac
     2401 gagacagcgc tgccaaatac gcagcatcag gaaagctatc tcctttctca tatctaagct
     2461 gagttagctt tttgactcca ccaatgtcgc tcatggcaac ttgactaagt cccaatcttt
     2521 ccctttcctc acgcaaccgc tgaccaatat catttttcat acaaaaacct tgacaggtat
     2581 cttttttgat accaaaatga tttcacgagc tattagatga tcacaatata ccactatgaa
     2641 acaagttctt cacgatacca gatcacgcat tccgcgtaac accgccacag gtccaagact
     2701 ggcacttcgg ctgtcccttg aggagcgagc cgtcattgat gaaatggcag ctaaagaaca
     2761 acgctcatcc tctaacatgg cgcgcatgat cttccttcgc ggcctagagc taacccagaa
     2821 agaacaaaac aaatcttcct gatcaggagg ctagtgggat gtcaggtata accatcaata
     2881 tcaatgtgaa tgccccctat gtatccctgc agaaatatgc agagataaca ggtatccctc
     2941 ttaatacgtg caaaaagatg ttggctgacg gtcgaattat tatccgcccc aaacgcgcca
     3001 aaatggaaaa gcctgaagta aaccttgtgg cgatgttaaa agacgctttg gctaacagct
     3061 aaaacaatga acagagcacc atcatgaaaa aaaacgctaa taatccatac tccaaatttc
     3121 gtaatggcgt agaacgccat gtacaccacg tcgctaccag tgcatcacgt agtaacagtc
     3181 gctataacct gaacgagacg cacgcaacac cggatggcca cgctgtaaaa caaatcggcg
     3241 agcatgcctg gctgattgag aaagctggaa tcgtggtcca caaatgccca cgcaatccgt
     3301 ttaccggaaa ccgcattttt gcattgaact gcggcgacaa tcacttcggg caggatttca
     3361 cattatacga agcacttcgc acggttgatc gtctgcttcg cgggcaaagt tttattaaac
     3421 aggctgattt ataacaggtg ctttatgacc aaagagcatg cacaaggtgt atttatccgt
     3481 tttattgatt ttcgcggtga actgttatta cgcgcatcag ctattgatgg agtcgtccca
     3541 tcagaaaaaa atgcagctac ttacgtttat ctgaacggca cgcgcctgac cgtagaactt
     3601 ccgtaccaga ctgtacacga aatcattagc gaagctgaaa aggcacgtca ggttaatggc
     3661 gataaaccct atatcgaaat tatctgcatg gattcagaag ctgaaattca gaaagcagat
     3721 taaagggtgt tgcaatggac aaagaatata aaacactcat caacaaagca cttgagcgtt
     3781 tttattttcg cttaagcgca tcaggcgttc atgctgaacg tgcagcccgt gactcattga
     3841 ccagggcaat ccggagtctg tatgacgtgg ctttttacgc tgatgatctg gatgcactta
     3901 acgaactttc cgagctgatc tgtgccgcag aatgcggaga acatattgaa ccgtataagc
     3961 tggggaatat cgcatgagta tatttatctc atggcttgtt ctgattattt cggtggcctg
     4021 cgccattggg attatgcgaa ttattaattc agtgaaaaag atcgagcgtt ttttctctga
     4081 tgaataacga tacaaataaa acatcaaatt aaataagaaa acgtgaaaac catccgtatt
     4141 aacggaggta ttcgcacacg caaataacgg agatacaaaa atgcacgcaa aagaagaagg
     4201 tatcatcaga gcactgaaag aaatttcaaa gatggaaagc gaagtagcga aaaaagccgt
     4261 ggccaatgct cacatggacg tcgcaaccca cacaatgata gtcgcaaaag tcacggcaga
     4321 ggctgccaaa atcatcgaag aacagggtgt ggaacttgcg cttctcaaaa ctaaaccagt
     4381 caccggactg gatttatctg acaccggacg ccttatttac actattggct cggagccaca
     4441 gcgatacacc attatcgccg gattacagaa caaatacctg atcactcctc accccataag
     4501 ggaatcaacg cttctgacaa atctccgcct gatagagcgc tctcaagttg cattcattga
     4561 tgacgcccga cacaccgtat ttaacgcata gggttactgg acaaaggggg tgcaatggca
     4621 attaagcatt ttcccgtcgt tcgctttacc tccagagggc gcgaatacga ggtcgacgaa
     4681 cgcctgatta ccactatcga caaacatcgt tcggaaaagg atgcacacca catctacctc
     4741 actgacggta cttacttctg cgccaccaac gtggcgcggg tgaatcttat ccgacaggta
     4801 caggagccac gcagatgacc attctggact acatcgctac tcatccgggt tgtagcggcg
     4861 gagagatcgc cgcagcactg aatactccaa ccacagccat taatgctgag ttacgccaac
     4921 tttggcgcgg cggcttagtc atcagaacaa accgcagcac aggtggtcgc gctcgcaaaa
     4981 ctggaggcca ggcttcttac cacgtaaacc cgatgccgtt cgggtgtagc aatccactta
     5041 ctcacatgtt taaccagcta ctaaaggaag ccagaacatg agcaccatca accaccagaa
     5101 gctacgcgaa ctggcatttg ccctgcaacg aatggcaacg cctcaaaaat tactggcatt
     5161 tcgcgcaatg ctctcgccgt ctgctgtgct ggcactgctg gatgagctgg agcacgccag
     5221 aaccacggct cctgccattc gcctgacact ccatcatgaa atcgctgatt tctgcgcgac
     5281 gttggaggca ccaggcgaac cggaaacgcc ggaagcaata cagcaagagc tgctgcaacg
     5341 cattgacaag gtttttgatt tttttctgaa ccagtaagaa accagaacat gcacacacaa
     5401 aaaaaccgct tgccatgccg caatcagtca ggttacattt ccgctgcacc tcataaaacg
     5461 ggtgccggga ttctcaaccc gatacagagc aaagcgcata accgcgccag cggttttttt
     5521 gtgcgtactg tattgccacg tctttttcgc gtcagaatta tggcggggcg tacggggccg
     5581 acttcggtcg ggccggattc tttgctctcc ggtgttgaga accctgtacg tctcgccacc
     5641 ccgagattct caactctgga tggtgagcta tttctatcac cgagcaaaga ggccacacca
     5701 tggcaaaccg caaacaacag cgcgcatacg ctgcgcgtcg tcacatccag actgaaatca
     5761 accgtagact tttccgcgca tcacgcgtcg cgcaaatcat gcacatcaat atgctgcatg
     5821 agcgcagcca cgcgctatcg aacatctatt ccgcctctgt tttcagctat ctggcggatg
     5881 atctgcgcga gctgcaacaa ctcttccagt agcaaaacaa actccattaa ttcctgttcc
     5941 gggcctttcc tgcaccttgc ggcgggaggc cttcgcacat ctgtagtaaa gagaattgca
     6001 gcatgattga cgctcatgac ttcacaagat gggtgcgcac acaggacacc cgtctggctc
     6061 ccgttcttca gggattattt gatctctaca tccgtggtcg tgacaacaga gcacgcacca
     6121 caaaaccaga gaatgcagac accctttatt tcacagtaga cgactgctac cgcgtggact
     6181 tcacaccaca cgggctggcg ttgcactgcc tgacaccaca cggagaatca ctgctggcgt
     6241 attacgactc cccggcctcc gtatttgcgg caatgctggc gcatcgcact gctggcgggt
     6301 gtgcctcgct gagtgaatac accgctgaat ttaaccgcct ttctgccctc ttctcgcagg
     6361 agtggcagcg cgtgacagga taccagccat gagtgagttt gcatggagct ggaatgaacc
     6421 acggccagcc attgatccgg ccagatttac ggagcacagg caggaaactg aaaccgacct
     6481 gcaacgcgcc atccgttact accttgaggc ggacaaaaag gcccgggaag aacaggaagc
     6541 gaaggaggaa gcctttttcg cacaatccgc catgggtaaa aaactcatgg catcccttga
     6601 ggaagccgga cagcgtgaaa agctggcaca gagcatcatc agtaagcgcc gggcaacaga
     6661 acaagacccg gtggcccgtg cctttgccac actgaaggcg cttcccgttt atctgcgaga
     6721 acctctgagc cgccacctct ctttcctgcg caagaagcag gaagccgatc gtcagaaagg
     6781 caaaaagagc tggcaggcgg aacgctatgc acgcggaacc ctgcgcaaaa tattcgaacg
     6841 tctggaccgc accgacagcc gctggctgac accgggttat cgctcccttg ccggacgcga
     6901 acgcctggac gatttgcttt acctgccgca gctcaacaaa caccagatac agacgctggc
     6961 caccatgacg gcggcgatgt tcagcagcac cttcgaaaaa ctctgcgatg gctttggcgc
     7021 gactgatggc gaactgacca tggatgtaac gctgaaggcg tatcagatgc tggcccgcat
     7081 ggcgttacac ctgcacgcca tgcctccaca ttatgacgca ctgacaacag acaaagaccg
     7141 gaggaacgaa ccggacacgg agctgctgcc gggcgcaatc cttcgcctga cctgtgcgga
     7201 atggtggaaa cgcaaactgt ggctgttacg ttgcgagtgg agagaagaac aactccgcgc
     7261 cgcctgtctg gtttccagaa aaacatcacc ctatctgagc caggacgcgt taagcgagtt
     7321 tcgcgcacag cgcgagaaaa cacgcgattt cctgaaaagt tttatgctgg aaaacgaaga
     7381 cgggttcacg attgatctcg aaacagtgta ttacgcggga gtaagtaacc cggttcaccg
     7441 taaggcagaa atgatggcca ccatgaaggg gctggaactt ctggccgaag cccgtggcga
     7501 caaagcggtg tttctgactg tcacctgccc gtcaaaatac cacgccacaa cagagaacgg
     7561 tcatccgaat cccaaatgga acggagccac catgcgcgac tccagcgatt acctggttaa
     7621 cacgtttttt gcggcggtcc gcaagaaact gaaccgcgac ggcctgcgct ggtatggcat
     7681 ccgcacggtg gagcctcacc atgacggcac cgtgcactgg catatgatgg tctttgctca
     7741 tccggaagaa atcgacacca ttgtgtccca cacccgcgat attgccattc aggaagatcg
     7801 tcacgagctg ggtgatgata ttaccccacg ctttaaggcg gagtacgtcg acggctcaaa
     7861 aggtacgcca accagctaca tcgccaccta catcggaaag aacctagaca gccgcgccgt
     7921 ggatggcatc gacccgaaaa cgggcaagcc acgcgttgac cacgaaaccg gaaaatcaat
     7981 ggccgagagc gtggaacgcg ccatcggctg ggcgcgcctt caccgtgtcc gccagttcca
     8041 gttctttggt atcccctccc gtcaggtgtg gcgtgaactc cgccgccttg ccagccagat
     8101 ggcacgcaac ccggaaggcc cgcaacggct gaaggatgac gcaatggatg cggtactcgc
     8161 tgccgctgat gccggatgtt ttgccaccta catagagaaa cagggcggcg tacttgttcc
     8221 acgcaaagac tacctgattc gcaccgccta cgacctcgca gatgagctga acgattacgg
     8281 cgaacagagt gtacagattt acgggatctg gtcgccgctc atcggggaat cctcccgtgt
     8341 gtgcacgcat ccggataact ggaagctggt aagacgtaaa ccggaagcgg aagacagcgc
     8401 ccgcgaaaat ggttttgacc ttcagggcgg ccctgccgcc ccttggactc gtggcaataa
     8461 ctgtccccgt gtacaggaaa cggacaacaa cgggacagaa cagccggaag aacggccagc
     8521 accgtggccg cagcttcctg acggcgttga tgtggatgaa tgggtgcgct cactgaaacg
     8581 gcacgaacgc cgggcgctga tgcgttcgct tcgtgacaaa caggcaaaaa acagcagtga
     8641 tgaaatgcag agctggacac agagccgcaa acagcagcgg cctttgcctg ataaccacga
     8701 attactcgct agagaatggc gggagtctgc tgaatctctc ggcctgcata tcggtgaaca
     8761 acaaatgcag cacctgttac ggggcggcag tctgtacgtt gacggcagca tcattgcacc
     8821 gcagggattt gaaattgtac gcaaaccgga tacccgcccg gacagccgaa tcacgcagct
     8881 ctggcagcgc ctgagccgta atcacggtgt aagcagcacg gagatccgcc ataacccgct
     8941 cgccagctat ctggcacagc tgggggcatc agaccctgaa gccgccacac gcctggcatc
     9001 cacacttcag caggaccaga acaccatgaa aacacccgtt accgtgcttt ctgacatgct
     9061 gcgcgccatc cgcgacgcag agcacgcaca gagaatcagt gaaaccactg aacgtgcccg
     9121 ccgcaaagca gacctgctgc ggggtggcct gaccagtgga aacaaaaaac agacagaaac
     9181 gggattcaca aatcccgtaa atgagcaaaa aacgcgccgc gatatatgaa gcgcacacaa
     9241 aacaggcaaa aacgggattt cagaatcccg taaacgatta attaatcaac ataaggaaaa
     9301 gcgacatgaa aatttgtatc gacgacggct ccaccaacat caagctggca tggactgaga
     9361 acagcgaacg ccgcaacgcc atcagcccga acagcttcaa gtcggaatgg tctgcgccgt
     9421 tcggtggctc gcagcctgcg aactacatgc ttgatggcgt gcgctatggt tttgatccgg
     9481 tcagcgatcg ctttgtccag acgaccgaca cgcaatacca gtacagcgat gtgaatgtaa
     9541 tcgccattca ccacgcgctg gtcaaatcag gcatcacacc acaggaggtg gatgtggttg
     9601 tcaccctgcc actgagcgag tatttcgaca caaacgcaca gccggacatg gccaacatca
     9661 accgcaaaaa agcgaacgtt atgcgcccgg tggagtacca gaacggcgaa gcattcacta
     9721 tccgtaacgt gcgggttatg cctgaatcca ttccggctgg ctttaaggca ctggctgaca
     9781 tgagtccgtt tgaatccctg ctgattgtgg atttaggcgg aaccacgctg gatgtggcaa
     9841 aggtccaggg acaactggca ggtatcagcc aggtgttttg cgatccacac gtaggcgttt
     9901 ccctgatggc cgatgccgta ctgtcggtga tggccactaa cggtatgcgt accagtcacc
     9961 acatcgccaa taccattatc gaacatcgcc atgatgaagc ctggttgcgc caacacatcc
    10021 acaatgacgc gcattacgcc agcctgatgg cggttattcg tgaaaaggaa gaaacactga
    10081 aacaacgcgt gatccgcgcg ctggcgggtt tttcgggtta cggacgggtg atggttgtcg
    10141 gtggcggggc ggagattgtg gcacccgcta tccgcgaagc ctgcggagtt aatgcgactt
    10201 tcatcgcgga cggggtgcca cagtttgctc tggttaatgg gctgtacgca atggacaagg
    10261 agtaaaccaa tgacgacacc aaccagacgg ataagtttct atctgaagcc cgccgccgtc
    10321 aagaacgaag gcgaagcatg cgcctggctg gacagcctta caccagaagc ccgcaaaagc
    10381 ggccaacgtg tggcttttct ggccgggctg gcacttctga aaatgaatcc ggcagaggct
    10441 taccgactgg ctgcatgggc tgatgatgaa gcgttatcag tgacacaaac caggacagaa
    10501 cgccccgcat cacagccagt atcaaccgca cagataacca gtcagatggc cggaaatatc
    10561 cgggcgttat ttcctgaata acacaacatc agggcgagtt cgccctgctc tccacctcag
    10621 aacataaaca aggagaacga cttaatgagc gaaatcaact atcaggcatt acgtgaacgt
    10681 tattcacctg tgccagtacc gaaatgccct atttgcggcg aggaaatgtc aattcagcga
    10741 atatctggag cacaggttgt ttatgcctgc tccggttatg gtgatgatgg agatttcaaa
    10801 attggccgaa ctcttgccga cgaacattat gaaaaatcac acgtaacagt gttggatgtc
    10861 gggatcctga agtattggcg ctacttgatt ggctggaaac caaagacaac cgaatcgctg
    10921 aactggaaaa aatcgccact gactatgcac ttaaattcca gaaagcacag gacgcattaa
    10981 agtacgccgc tttgctgcat agcaggacag cgcagctaaa atactaactt aaagcggaag
    11041 attaatacat ggtgagtcgg ggtaatttgt ttttatccca acaaataaac aaaaaagtgg
    11101 actatctctg tagtatctta ttcagcatcg tctcgtgcag tctgtgcata cgtcacaaca
    11161 acgcccaatg cattagtttt cttttcaaca tagataatat tgtcatattc atagttacca
    11221 tacggatagc aaataagaat atttttatgc agttcgggat catcaacaaa cgctccggaa
    11281 cagcttcttg aactaccaga tgtatactca tacggtgcaa catatgatga tgttactcct
    11341 ggtaaaaaat aagtcaccat cataagaggg ataatcagga atatccctgt aagaatgcaa
    11401 gagccagcat aaaccttcag gtattccgac agtcctttcc agccactttg cttcactacc
    11461 cccctcttca ccagaaacaa ggagaagaaa aaggacatac ccatactaca caagatgtaa
    11521 taattggata tacgcgaatt aagatatgta atcctgtaga tatctgccct ccaccagtaa
    11581 aagaggaaaa taatccctag cactgaaaca gtcatgcaaa tcaaataagg atatgaatct
    11641 ttcttcatgt cttgcgccca ttaaaatttc cctgctacga acaaatttac catccgtttt
    11701 ttgcgcagaa aacagcacga tgcacactgc acgatagtgc acaaatttgc acaatttttt
    11761 tgaacgactt tttgcccttc cggcccccgt ggcggctgga tccgtcaagg atccgtgcgt
    11821 gcacaaaaaa acgcgttttt tctgcgcgca ggtgacgggg gaacagcccg cgtttcaggg
    11881 ggtaaatagc attccctgaa cgatgtcgca gagatacaac agaatggctg tatttctcac
    11941 gctgagcgtg aaaaagacgt gagggctttt gatttgatgg ggtgaaaggt aaggccgtca
    12001 aaatcgcact gagacggcga gaacatgcag tcaacgcggt gggattgcgt aagagtctga
    12061 ctgtcgatga tggcaatcag caggaaagcg tcgtgaaatt atctgactga tacaggagct
    12121 ggagagtcgg ggcataaatt ttttatgccc cggcgaagca gcagacaagc gaagcgcgtc
    12181 aggatgtggg ctgggtgtct aacagtgcgt aagggttaaa gcggatcacc tcttccccaa
    12241 gccagtcatt gatgtgcttc atggcctcca tgacgggcat cagctcgtta attgcgtaaa
    12301 cccgcgcggc cttctccaca tcaccaaacg cacttttttc gcccggcatc gcccccatca
    12361 gttgcggcgg aacgcggtgc gcagccagca catcatcacg ggatgccgcc ttaacattca
    12421 tgaactcatc ctttgcggtg atctgctgga acggcaaaat ttgcaccccc tctttgcccc
    12481 cgttgggcgc atggatgagc acgtttttaa acgcgccacc accacgcgca ccctgtaacg
    12541 tttctttcag ggagtccatg ctttcgcggt ttacctgcgc tgcaccgatg tagatgatgc
    12601 acccggcgtg ggatccattg tcgtaataca gttttctgaa catgtccgcc gaatgagaca
    12661 ggctggccga gagtaatgcg ccaagatatt ccggcatgcc gtaaatttcc tggttaatat
    12721 ccggattcat taggtggcac actttgccag ggcgaaactg gaacgcgtcc ttgccatcct
    12781 gcacatacca ccatgattca agatcgcttc cgcgtcgcat gtatttcgcc agtgcgtgcc
    12841 gtaatttaag cggttcgccg agcatattgc ttctaagctc aaggaatgcg ttaccgaaca
    12901 caaaccagtc cagcgccagc gccgagaaat cctgccggga aagcagcggg tgcgggatgt
    12961 agcaaccgag taatacattg cgcttaaagt aaagcgcaga ctgatgccag gacgtttgcc
    13021 gggctgctct tgccagaccg taccagtcca ccggggtttc ataccaccgc ccgttatcag
    13081 cacagtacat attgtccagc aggtcatgcc cggtcaggcg ataaggacca tcaaatgtga
    13141 atgcactgag cgatgattct ttcctgagcg catcagcgag atcaatgcgt gaactcatgc
    13201 gcactttttt attttttctg ctcatcagaa ctccataacc gtgaaacgct cgttttctcc
    13261 ttcgccgcca atcggttcgt taatgacagc aagcatggtt gcccacgcaa ggtcgccgtg
    13321 gctgatcccc ctcgcgcggt ccgtttcgta agtgataaag ccgcccggtg ttttcacctt
    13381 acgcacggcg ttaaaggccg cgaccagctc gcgttcggcg cgatcgtatt cccaccgtcc
    13441 ggcacgcatt atttgcagca ttttcagtac cagcgaccgt ttggatgaca gcgtgaaggt
    13501 gtacggaata gcagcaggga aaaaccgctt cactatctga taaacagcct ccccgttccc
    13561 gcccgtcaca tcaatgccga tgtgttccac gttgtagcga cgcgtgaact cttcaatgac
    13621 tctggcctgt tcttcaaact ccagcccctg aacgcgtcgc gtctccaccg ttcgaaaacg
    13681 gccaccagga acagccggag gaaacaccac ggacacagcg ccgctgtcgc cgttgccact
    13741 gctgccgttt gcgtcatacc caatccatac cggacgattc cccatcgggc ggggagcaaa
    13801 aggtttccag tctttccagt cgtcgtatcc gtcaacaccg cagccaatca ggatattcag
    13861 gttaaatgcc gattcccctt cgcgaacaaa ctcacacata tagagattga ggaactcgtc
    13921 ttcggtgttt tcatcacgaa tttcatcaat atcggtgtgt ttccagccgt gattaaccac
    13981 atcttccagc gtgacaattt gccgccacgt ccggtcaggg cagataagcc cgttatgcag
    14041 cgttttccag tccacagaaa aacgctggcg tttatgcgtg gcctttttct cgttccagcg
    14101 gtcgccgttc cagtaggcgt atgcctcgtg cgtttcggtg gatggcgtgg agaagtaggt
    14161 gcgccgcagt ccgctgaggg ttgccatagc gccagccacc ttgcgcagtt cagcaaagcg
    14221 actgacccag aagaattcat caaaataaaa attgcccgta taggactgtg ccgacgcagc
    14281 agaagtgccg agaaaatgca gctctgcgcc gttggagagg atgattttat cgcccccttt
    14341 cagctccaca tcaacttcag ccgcggcctt ctgaataatg cttttaaact ggaacgcctg
    14401 acgacgcgac gcagacaaaa aaatctggtt acgctggtaa ggttgcgcca catcgtcacg
    14461 cagcgccatc agcagagctt cctgtgcaaa ataccaggtc gccccaatct gtcgggattt
    14521 caggatcatc ctgttacgta tccctgcttc cctgcaaagg gtcagggagt caaaccagcc
    14581 ccgctgatgc cactccagcc tgctgatgat tttttcccgc agtgcggcaa tctgtttcgg
    14641 cgtgaaatga tttttgagtt ttttcgcccg gcctttcttt cctgcggcca tcacatccgg
    14701 ctggccatca tgcagctttt taagctgccg ggtcagcagg tctatttcct taaagtcacc
    14761 gcctgtttta ttctgttttt cagtaagctg gatgaggcgc gcatcgatgg actgcgtgac
    14821 acgctgcacg ggtggcgttt catcccactg gtcacgtttt ttccacgcat aaatcgtgtt
    14881 cgggtttatt cccatcagac gtgatatttc tgcgggcgga taaccctgcc agtaaagttg
    14941 ccgcgcacgc tggcgcacaa aagcgtcctg aatcattgct ccccctgagt aattacagga
    15001 agattacccg cgcgcgaaac tgttctcctt aaccctctgt tctgactgtt ttcttacaac
    15061 aaaagccctt tgtatcagcc tgttacgctt tgccatcatg actgaagaac cagtcagagg
    15121 ggcaaaaact atggctaatg aaaaaaagac atcccgcaaa aagtttcgcg tggctgtctc
    15181 cggatcaact gttgatggcc gtgaaatcag tccggtgcat ctgcgtgaag ccgccgagaa
    15241 cttcaacccg gatgtttacg ctgcccgcgt gaacgttgag cactatctct cgccatgccc
    15301 gtcaagcgaa ttttccgcaa tgggcgatgt caccgcactg agtacggaag acattacgga
    15361 aggtccgctg gccggacgta ctgcgctgta tgcagaaatc gaaccgaccg agcgcatgaa
    15421 gcagcttgtc gcggacggca agaaaatcta ttccagtatc gaactgcacc cgcagttctc
    15481 cgttaacggg cgcgcctatc tggtcgggct ggcgatgacc gacaccccgg caagcctggg
    15541 cactgagcgc ctgaaattca cggcacagca acgtcaggcg gtgatgacgt tcaacagtat
    15601 ccagggtgaa gcgccgctta tctccgaagc catcgagtct gaaatcatcg aaatggcaga
    15661 acaacgccag gaagaaggca cccagtggtt taaccgcgta atggggatta ttggccgtgg
    15721 ccgcaaagcg gatgacgcca gtttttcccg tattcaggaa gcggtggaag gcgtcgcaac
    15781 gtcacaggcc gacattatcg accgttttaa tgtgctggaa acccgccatc agcaggaccg
    15841 ccagaaaatt acgtcactga ccacagagct ggcagcactg aaggaaaaac tgcgcacgca
    15901 ggacggcgat ccgcagaacc ggttcaccgc aacaggtgca gcctccgacc agctggctga
    15961 cttctgataa gacaaaggag caaatttttt atgaatctgg tgatgtcaga tattacccgc
    16021 aacaagctgg gttgctatat ggcgcagcag gcgtcgctta ataatattcc ggtttccgca
    16081 ctggtatcgc gatttaccgt ggaaccctcg gtgcagcagc gttttgaaaa cgcctcaaag
    16141 gaaagcaccg aatttacaaa aagaattaac gtgatcggcg tgaccgacca gaaaggcgaa
    16201 aaaatcctcc tggacaccac cgggccaatt gcacgcacga ataccagtta tgacggcaca
    16261 aaacgccgta acccgaataa cgtggttgat ctgaaaaacc gcaaatacca gtgcgaacag
    16321 gtgaactacg acacgtttat ttcatatccg cagcttgatg cctgggcggc acatcctgat
    16381 tttcagtccc gcatcagcgc acagattgcc cgacaggtgg cgcttgaccg catcatgatc
    16441 ggtttcaacg gcacgtctca cgcggatgag tccaacttca gcaccaacaa gctgcttcag
    16501 gacgttaacg tgggatggct ggagcacatc agaaccgacg ccagcgaacg cgttatgaat
    16561 gacgtgacgc tgacctcccg caacatggac aacaccgtgg cgcacgcggg taagtatgcg
    16621 aacgctgatg cactggtaca ggacgcgcgt tcatccctgc tggatgaatg gcacaaggaa
    16681 gctgacgacc ttgtggtgat tatggggcgc aacctgttta actcgctgcg tctgcccgtg
    16741 ctgaacagca tcagcggcca gaatccaaat gcggaattac ttgccgggca gctcatcctg
    16801 tcatcgcgca ccattggcgg gctgggcgtg ttccttgcgc cgttcttccc ggatgcaacg
    16861 atgctgatca cctcgttcaa caacctgtcg atttactggc agaaaggttc aatgcgtcgt
    16921 ctgatgaaag acgaaccgga atacaaccgc atcgccacct accagtccat caatgacgct
    16981 tatgtcgttg aagactatgg caagtgcgcg atggtcactg gcctgaagtt cgccgacagc
    17041 taatcaactc acggcgggca tcatgcccgc ctgtaacgga gagaaaaaat gattactcct
    17101 gcacagcaac actggcagaa cgtgatggca cagcgcgcag gccgggcgaa tgaaggcgtg
    17161 gaccacgccg cgcgtaccgc gcatgaagag gtgctgtatc gtctgcgtct ggcacaggcc
    17221 cggcttaagg gcgtacaggc cagaagcgcg aaagccgcca tcaaaaaaga gttgttgccg
    17281 gacttttccg gctggattga gggaacgctg gaggctgacg gcgggcagca ggatgaagtg
    17341 attgccacgc tgatggtgtg ggcgattgac tgcggcgatc ttccgcttgc gctgcgtatt
    17401 ggtgcgtatg tggtccgtca caacctcatc atgccggata actttggccg tactgctgcc
    17461 acggtactga ccgaagaaat ctgtaacccg gtattgacgc aggccgggac ggatgccgac
    17521 gcggatttgt ccacctttat cgaaccactg gacacacttt gggaaattgt cgccaaccag
    17581 gatatgccgg acgaagtgcg cgccaaatta tgcaaggcgt gcgcctttgc ccgccgtggc
    17641 ctgagcgatg cggacagcat ggcctcatca ctgaagctgc tgcgcgaagc gatgcacctg
    17701 aacccgaacg caggtgtgaa acgcgagatt gcaacccttt cccgcgccct gaaaaaagct
    17761 gattccgcag ccgcaccaga agatgccagc acaccgcaga cgcaggacga aagcagcaaa
    17821 agtaaaaaga caacgcggaa gcctgcaaca cgaaaaacca ccgcgacgca gaaggcgaag
    17881 cgcggttaac gactgacccc gtcagcgggc ggcgtgcgcg gtgttccggt ttgactccgt
    17941 gaccgtttac accgcgcacc caccgcccga ttttttcagg agtgaacccc atgagtatgg
    18001 ttgccagaac caaccccgga cccgcagagg acgacatcac cgataccgat gatggtgata
    18061 cccgtatttc agcgggtgca ttctggccgg atattgtgct gcgtgagctg cgtctggcga
    18121 tacgactgcc gggccgcgtg accacctccc gcctgctgca taccgccacc ggggccgtgg
    18181 cacacgttac ccgcgagctg gaagcgtggc agcaggaaca gcaggcggct ggccatcaga
    18241 cgctggccga tgttccggcc cccgtaatta acggagaaag cgtcaatctc tggcactggc
    18301 gcaatgctgt ttataccgcc acacgcgccc tgattctgga gcgttaccgc gatgcggaca
    18361 caacggataa gggcgaccgc cgggcggacg cactggatat acagacatcg gatttgtggc
    18421 gcgatgtgag ctgggccatc tctgacattc tgcgccgccc gcgaatcttt gcggagctgt
    18481 gctgatgaaa gtgaaggcac tggaaggcga caccgtggat tcgctctgtt tccggtacta
    18541 cggcacgacg cagggcgtca ccgaaaaggt gctggatgcc aaccccggac tctgtcagca
    18601 ggtatttctg gacgccgggc aggaagtgga gatgccggag ccggagaaga agaaacgaga
    18661 aatgattcag ttgtgggggg agtagcagtg agcaccattc aaacagggat cacagagcag
    18721 gttattgcgt ggctctttga ccacctgcca acggtgtatg cagtaggcgc ggcagtcagc
    18781 atttccgcgc tgatgagtct ttatgacgga cgaacactgg ttcagaccgt aacgggatcg
    18841 ctggcgtgcg gcgttcttgc catggccgtg gccgggtcgt tgcgcttctt cgggtttcct
    18901 gaagatgccg tgacgtttat cggcgcatca atcggtttta tgggcgcaga gaaagcacgc
    18961 gacaaggtta ttgcggcctt taatcgcagg gtgaaggaga aggacgaatg agcaacacat
    19021 ttaaattcag cagccggagc gaaaagaatt tgcagggtgt aaatcctgat ctggtgaaag
    19081 tgacccgacg ggcactggaa atctcggaag tggattttgg tatcaccgaa ggggtgcgca
    19141 gccgttaccg ccagaagcaa cttgtggcca cgggtaagag ccagaccatg aacagccgcc
    19201 accttacggg gcatgccgtg gatgttgtgg cttatgtcgg cagccaggtg tcatgggaat
    19261 ggccgcagta cgaaaaaatc gcagcagcat tcagacaggc cagccgggaa ctgaatattc
    19321 cggtggaatg gggcggagac tggaagaccc tgaaagacgg accgcatttt cagttaccac
    19381 acggagccta tccggcatga agctctggcc cacgctgggt gtcgctttcc ttctgattgc
    19441 cgcatgggga acatccatgc gtctgtcgtg gtcgctgggt cgggagaacg ccagaaacga
    19501 agcgcaggcc agcgccctga aaagtaccgc cgacaccctg aatatcatca gcgccggggt
    19561 acaggatatg cagcaggtgc tggcacaact ccgcgcggaa aatcagcaac gcaatcagga
    19621 cggagaggcc agacgtgaac agctacgcaa cgatattgca aaagatgaat gcgcccacgc
    19681 tttgcctgac gctcgtttta ctgacaggtt gcgcaggcac gcagaacgcg ccacggccag
    19741 cgccgtcagt ccggcttata ccgcagacgc tgaccatacc ggtaatgcct ccccccttcc
    19801 ctgatactcc cacatgggga aatctcggta tatggggcga ccgccttctg gatgcactgg
    19861 aaacctgtaa cgcggataaa cgggccattg aattactgga acagcgccgg ctgcaacgac
    19921 tgaacaacga ggacaacaac catgctgaaa actgattccc tgcgtgaagc catgacccgt
    19981 tcatgccgat ggtgtcaggc caacccggaa aaattcacca ttttcgtgga gagcggcaac
    20041 attgaaacga ccggagaaac gccctcgttt gtttaccgct atcagatggt gatgtttgtc
    20101 atggattacg ccggggagct ggacgacctc acgctgccgc tactggcgtg gttatccgaa
    20161 aatcagccac agttgttgct caaccctgag cgtaatcagg acatcaaatt ctccgccgtt
    20221 atcaatgacg atgacagcgc cgatctcctg tttacgctcc ccctgcggga acgcgttcgc
    20281 attacgcgca acagtcaggg gacaccgcag gcagaacacc tgccggagcc aaaaccccgt
    20341 ctgccctctt ccgaaggcga ctggtcgcat gtattccagg atgtgacgtg gggtgaaagc
    20401 gatggataag gcattcaccc gcgtggatga aacctttgag gccatccgcg acagcctgaa
    20461 tcagcaggcc atcaataaca tcgccagaaa gctggcacag gatttacgcc gcgcccagca
    20521 ggcgcgcatc cggtcacaga aagcgccgga cgggaccgca tggacaccac gcagacgccg
    20581 cgtaacccgg atacaggagc gcattcgctt tatctggaat aacgaagcac gcacgctgaa
    20641 aaactggcat cacgacacgg ggaaatacgg gcgaaccatt accgggtggg atgaggataa
    20701 aaacaatatc cgcacgtttt accgggatga catcgaccgt tttctggaaa tacgcacccg
    20761 gcgcatcaac caggacagca caaagcgcgt ccccatgttc gtaaaactgc gcaccgcccg
    20821 ctacctgaaa gcccgtgcag atgcttccgg tgtgacggtg ggttacagcg gcgtggctgc
    20881 acgtattgca cgcgttcatc agttcggtga gcgcgatcag gttgcgccgg gcattttcac
    20941 cgattacccg gtacgtgagc tgctgggtat cagccaggca gatgaacgcc tgatttataa
    21001 cacggtgctg ggccggattg cggaggctgt acggtgagcg cagaactcat gcgactgctg
    21061 agcaacatca tccgcaccgg gattatctct gaagttgatg agaagtcctg gtgcgtgcgc
    21121 gttcgcagcg gcgaactgga aacaggctgg ttgcgctgga acaccacgcg cgcgggagcc
    21181 ttcaatgtgt ggctgccgcc atcacccggc gaacaggtgg taattgcctg cattggcggc
    21241 aacccggaaa ccgccatgat aattggcagc ctgtggagtg atgccagtcc ggcacccggc
    21301 aaaagcctga aagaaatcgt ggtcagcgcg ccggacggcg cggtgttccg ctacgacgcg
    21361 gacgcaggcg cactgagtgc cagcggcatg aaaacggcca ctttgcaggc atccgtcagc
    21421 gtgaaactgg atacgcccgt cgtggaatgc acaaaccttc tgaaaacagc cgagattgac
    21481 gtcacaaaag ggggaaagat gagcggcaat atcacgcaca gtggcggcga tttcacctca
    21541 aacggcatca cagtgcatac gcataagcac ggtggcgtta aaggtggcag cgattcgaca
    21601 ggaggcccgc agtgacaacc cgctacacag gaatgaatcc ggacggaacg ggaaacctga
    21661 acgatatgga gcacctgaaa cagtcagtca gggacatcct gaccaccccg ctggcaagcc
    21721 gggttatgcg acgggaatat ggcagccttg tgcctgattt gattgacgaa cccatgaata
    21781 acaccacgcg tctgcaatgc atgagcgctg ccgtgattgc actgacacga tgggaacccc
    21841 gcattgccct ggacgccatc gacgttgtct ggaaggcagg aggccgcgcc ggggtgacgc
    21901 tgtcgggcac tgtcatgcag accatgcaga atgttgaatt aaccatcacg ctgagagagt
    21961 aaatcatgcc tgccgttgac ctttcccagt taccggaacc cgccatcatc gcggagcctg
    22021 actttgaggc aattctggct gacacaaagg ccatgatgat tgcgtcctat cccgccgaac
    22081 agcgtgaagc cgtttccgcc gcgctggagc tggaatcgga accccttaac gttatcgctc
    22141 aaaccatgtc gtttcgtgaa atgctgttac gccagcgggt taacgagggc gcacgcgcct
    22201 gcatgttaag ccacggttca gggacaaacc tggacaacct cgcgggcaat atgaacacaa
    22261 agcgcctggt tatcactccg gcaacggata ccaccgacgc ggtgatggag agcgacacct
    22321 cgctgagact gcgggcgcag cgggcgtatg acggcctgag tgttgcaggc ccgtcaggcg
    22381 catacgagta ttttgcacgc agcgccagcg gtctggtgcg tgatgcgcgg gccatcagcc
    22441 cgtctccggc ctgtgtgacg gtttccatcc tgtccacaga gggcgacggc acagcaacgg
    22501 aggcgttgct taataccgtt cgcgctgttc tgaatgcaga ggatacccgc ccggtggccg
    22561 accgccttac tgtacagagc gccagaatcg tgacatggcg gctgaatgca aaactgtact
    22621 tttaccccgg cccggaatcc gaacctattc tggccgcggc ggaatcgtcg ttcaggaagt
    22681 ggctgtctga gcaggggctt atcggtcagg acgtggcgtt gtcagccatt gctgccgcac
    22741 tgcatgtgca cggtgtgcaa cgcgtggaga taatcgaacc cacacagaat atggccatca
    22801 gcgacataca ggcggcgcgc tgtgagtcat tcaccatcag cgaaggtggg cgtaatgagt
    22861 aattcactgt taccgccatc agccagcaat ttcatgcgtt gtgccgaagc tgtcggaacg
    22921 cgcattacag acattccggt agacctcaac acgctgtggt cgccggacac ctgcccggtg
    22981 catctgctgc cttatctcgc ctgggcattt tccgttgacc gctgggatcg caactggccg
    23041 gaagagacaa agcgacaggt tattcgtgat gcatggctga tacaccgaca caaagggacc
    23101 atcagcgcac tgcgccgggc cattgagccg ctgggatacc tcattcgtgt gtctgagtgg
    23161 tgggagttcg gcggagaacc gggaacattt accgttgaag tcggcacgct ggacagtggc
    23221 gtgacggagg aaatgtatct ggaaatggag cggttgattg ctgatgcccg cccggtcagc
    23281 cgccacatga cagggctgaa tatcattcag gagatcccgg gggatatttt cgcggcggca
    23341 gcaacttacg acggtgaagt cattaccatt tatccggacg attaagcatg agtaccacaa
    23401 cacgaaaatt taaaaccatt atcactgata ctggtgccaa aaaattagct caggcagccg
    23461 cgccagatgg taatcctgtc cacctgactc acatggccgt gggcgacggt ggcggcacgt
    23521 tgcccacacc agacagtaag cagacccgtc tggtgcatga ggtgtggcga catactgtta
    23581 atcgcgtcat cctggacgca acacatcaga accgcattat tgcggagctg gttattcctc
    23641 ctgaaacggg cggattctgg atccgggaaa ttggtgtatt tgatgagcac ggcgatttga
    23701 tcgcggtggg caatactgcc gaaagttaca agccagccgt tgccgaagga tccggacgtg
    23761 cacaaacatt tcgcaccatt ctgaccgtat ccagcactgc caccgtggcg cttaccgtgg
    23821 ataacaccat ggttatggcc acagtggatt acgtgaatga caaactgaaa gaacatgaac
    23881 agtcacgacg tcacccggac gcctcgctga ccgcaaaagg ctttgttcaa ctcagtagcg
    23941 ccaccaacag cacgtctgaa gcactggccg caacgccgaa agcggtcaag gcagcctatg
    24001 accttgctaa cgggaaatat accgcacagg acgccactac agcgcgaaaa ggcattgtcc
    24061 agctcagtag cgccaccaac agcgattcag aaacgctggc ggcaacacca aaggcggtaa
    24121 aggcagccta tgacctggct aacgggaaat acaccgcaca ggatgcaaca acagcacaga
    24181 aagggataat ccagctaagc agcgcgacca acagcacatc tgaaacgctt gccgcgacac
    24241 caaaggcagt aaaaacagcc tatgacaatg ctgagaaacg tctgcagaaa gatcagaacg
    24301 gtgcggatat ccctggcaaa gacaccttta cgaaaaatat tggtgcctgc cgtgccttcg
    24361 gtgggtcagt aagcacaaca acaggaaact ggacgactgc acagtttatc gagtggctgg
    24421 attctcaggg agcatttaac catccatact ggatgtgcaa gggttcctgg tcttatggca
    24481 ataataaaat cattactgat actgactgcg gtaatattca tctcgccgga gctgtcattg
    24541 aagtaatggg gataaagtca gcgatgacga tccgcattac cacaccgacc acctccactg
    24601 gtggtggaac aactaacgcc cagtttacct atattaatca cggaacagat tattcacctg
    24661 gctggcgaag ggactataac tccagaaata agccaacggc atcagagatc ggggcgttac
    24721 cgtcaggtgg aacagcagta tcatcagtta atctggcttc aaaaggtcag gtaaccgcgc
    24781 tgacagacaa tacgcagggg gcaacaggtc ttgagttata cgaggtgtat aacaacggat
    24841 atccaacagc gtatggaaat atcattcacc tgaaagggat gacagccgtt ggcgaaggtg
    24901 agttactcat cggctggagt ggtacaagcg gtgctcatgc tccggcattt attcgttcac
    24961 gacgggatac gaccgacgca aactggtcgc cgtgggcgca gctttacacc tcggctcatc
    25021 ctcctgcaga gttttatcca gtcggtgcac caatcccgtg gccatcagat accgttccgt
    25081 ctggttatgc cctgatgcag gggcagactt ttgacaaatc tgcctacccg aaacttgcag
    25141 ccgcttatcc gtcaggcgtg atccctgata tgcgtggctg gacgattaag ggcaagcccg
    25201 ccagtggtcg tgccgtattg tctcaggaac aggacggcat taaatcgcac acccacagcg
    25261 ccagcgtatc cagtacggat ttgggtacga aaaccacatc gtcgtttgat tacgggacga
    25321 aaacaacctc actatttgac tatgggacca aaactaccag taacgccggg agtcacacgc
    25381 attcaatacc aacatcagat ggtgcaaacc agtgggggaa aattccactg acatctgggc
    25441 gtctggcaac tcaatataca tcagcaacaa caggttcctc tggctcacat tctcatactg
    25501 taggtattgg ttctcatcag cacactgtcg gtattggtgc acacacgcac tccgttgcga
    25561 ttggctcaca tggacacacc atcaccgtaa acgctgctgg taacgcggaa aacaccgtca
    25621 aaaacatcgc atttaactat attgtgaggc ttgcataatg gcattcagaa tgagtaaaca
    25681 atcacgaacc ctcaaaattt ataatctgct ggccggaact aatgaattta ttggtgacgg
    25741 tgacgcatat attccacctc atacaggtct acctgcaaac agtaccgata ttgcaccgcc
    25801 agatattccg gctggcttcg tggctgtttt caacactgat gaggcatcgt ggcatctcgt
    25861 tgaagaccat cggggttata ccgtctatga cgtggcttcc ggcgacgcgt tatttatttc
    25921 tgaacttggc ccattaccgg aaaatgtcac ctggttatcc ccggaagggg agtttcagaa
    25981 gtggaacggt acatcctggg tgaaagatgc agaagcagaa aaactgtttc ggagccagga
    26041 ggcggaagaa acaaaaaaca gcctgatgca ggtagctagc gagcatattg cgccacttca
    26101 ggatgctgta gatttggata ttgcgacgga agaagaggca tcgttactgg ctgcatggaa
    26161 gacatatcgg gtattgttga atcgtgttga tacaacagta gcagcggata ttgagtggcc
    26221 agtcgcccca caataaaaag aaaaagccat cgatagaaat atcgatggct ttatgtgctc
    26281 tatttataca atacaacacc gctcttttta gttatatatg tgcagttcga tggtatatct
    26341 ttatttataa aagacattgc gcctattttt acattatccc cgattttacg tgataatcca
    26401 atgatgcaac aattagctcc gatatcaacg ttactaccaa tttttactct tgaaccaggt
    26461 atgtcaccat ctatctgtcc aatcgtagta ttctgtcgta ataccagatt ttcacctgca
    26521 tcaacagcaa aatgaacaac aattccagca tgatggggaa ttgttaaccc ttttccaata
    26581 tttgctccca atcctatttc acaaccaaat ttgttaatta ttttactgtt taactttttg
    26641 gctgcttttt tatgtaattt attaccatta atatacattt cgttagccaa ccgccaccag
    26701 aaaaggaaat tccggttacg ctgctttttc tctcttaaaa gcctccagat atccatacgt
    26761 ttccgccgaa ttacttcatg tttccag
//
LOCUS       DAZFYC010000068        26196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFYC010000068 DAZFYC010000000
VERSION     DAZFYC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.068"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHKW5_003042"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHKW5_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO7512394.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHKW5_003043"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHKW5_003043"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO7512395.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHKW5_003044"
     CDS             complement(941..1408)
                     /locus_tag="ACHKW5_003044"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO7512396.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKW5_003045"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKW5_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO7512397.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKW5_003046"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKW5_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO7512398.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKW5_003047"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKW5_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO7512399.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKW5_003048"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKW5_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO7512400.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKW5_003049"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKW5_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO7512401.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKW5_003050"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKW5_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO7512402.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKW5_003051"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKW5_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO7512403.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKW5_003052"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKW5_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO7512404.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKW5_003053"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKW5_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO7512405.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKW5_003054"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKW5_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO7512406.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKW5_003055"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKW5_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO7512407.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKW5_003056"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKW5_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO7512408.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKW5_003057"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKW5_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO7512409.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKW5_003058"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKW5_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO7512410.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKW5_003059"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKW5_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO7512411.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKW5_003060"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKW5_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO7512412.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKW5_003061"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKW5_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO7512413.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKW5_003062"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKW5_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO7512414.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKW5_003063"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKW5_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO7512415.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKW5_003064"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKW5_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO7512416.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKW5_003065"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKW5_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO7512417.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGHGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHKW5_003066"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHKW5_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO7512418.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKW5_003067"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKW5_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO7512419.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKW5_003068"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKW5_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO7512420.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKW5_003069"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKW5_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO7512421.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKW5_003070"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKW5_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO7512422.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKW5_003071"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKW5_003071"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO7512423.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKW5_003072"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKW5_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO7512424.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHKW5_003073"
     CDS             15548..15916
                     /locus_tag="ACHKW5_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO7512425.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKW5_003074"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKW5_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO7512426.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKW5_003075"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKW5_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO7512427.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKW5_003076"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKW5_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO7512428.1"
                     /translation="MNIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKW5_003077"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKW5_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO7512429.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKW5_003078"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKW5_003078"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO7512430.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKW5_003079"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKW5_003079"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO7512431.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKW5_003080"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKW5_003080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO7512432.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22618
                     /gene="dprA"
                     /locus_tag="ACHKW5_003081"
     CDS             21497..22618
                     /gene="dprA"
                     /locus_tag="ACHKW5_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO7512433.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTVFV"
     gene            22593..23063
                     /gene="smg"
                     /locus_tag="ACHKW5_003082"
     CDS             22593..23063
                     /gene="smg"
                     /locus_tag="ACHKW5_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO7512434.1"
                     /translation="MFDVLYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNALL
                     WLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLETR
                     EMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHKW5_003083"
     CDS             23092..23634
                     /locus_tag="ACHKW5_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO7512435.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHKW5_003084"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHKW5_003084"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO7512436.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHKW5_003085"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHKW5_003085"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO7512437.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHKW5_003086"
     CDS             25031..25288
                     /locus_tag="ACHKW5_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO7512438.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHKW5_003087"
     CDS             complement(25264..25818)
                     /locus_tag="ACHKW5_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO7512439.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6741 a         6209 c         6784 g         6462 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aggcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaaggaaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcatggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgtt catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgctaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatattt acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca gattcaggtg ctcaaccttg aaactcgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taacgcagag ttcgaactgg atgatctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgaaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgcc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatcca ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgtcctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgtcggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcggcaaat tacgagcagc ttaaacccta tattgatgac accatgctga ctgatgcgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcaa caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcatcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt accttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca
    24481 agcggttaga agatggacgc ttgcagggtg acaataccga tggtataggt ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtccgg gtttacgtat tctgcttatc ggcgctggtg
    24601 gagcatctcg cggcgtacta ctaccactcc tttccctgga ctgtgcggtg acaatcacta
    24661 atcggacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc tctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tctggcatgg tgtgagcagc
    24901 gaggctcaaa gcgtaatgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 ttcttctctg gcacggtgtt ctgcctgacg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg acgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgagagtctg gcgtatcgtt ttactggaga tacgccagaa cagtggttag cgagttttcg
    25201 tcagcatcgc tgggatctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggc tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt
    25321 attcgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 ggggctaccg agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaaca tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg
    26041 gtcggaacaa cgaagagaaa aaatcctgaa attcagggtt gactctgaaa gaggaaagcg
    26101 taatatacgc cacctcgcga cagtgcgcta aagcgcgtcg caactgctct ttaacaattt
    26161 atcagataat ctgtgtgggc actcgaagat acggat
//
LOCUS       DAZFYC010000069        26190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFYC010000069 DAZFYC010000000
VERSION     DAZFYC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.069"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..647
                     /locus_tag="ACHKW5_003088"
     CDS             <1..647
                     /locus_tag="ACHKW5_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO7512440.1"
                     /translation="DLARRSGQILSISARTVHTNDKFSYSYGLEETLEHLPCETGDRG
                     ELTHVYAVARLKDGGVQFEVMSLADVEKVRALSKAGSSGPWIDHFDEMAKKTVIRRLF
                     KYLPVSIEMQKAVVMDERAEAGLSQDNAAVITGEYSVVDDERQHLSPISDSEREEARE
                     YIIAILNSLDPSAEDAKTMFKRAENEINTMAEKLGDEYHQKFMMTLNDMRPEFE"
     gene            697..945
                     /locus_tag="ACHKW5_003089"
     CDS             697..945
                     /locus_tag="ACHKW5_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7512441.1"
                     /translation="MKGALGKKELLAVVPLSWSTIDRMERAGEFPKRWYITDKRCAWN
                     RDEVERWLDERQAASPAEFQGKKPPVQQRVYRPVSNAA"
     gene            1103..1354
                     /locus_tag="ACHKW5_003090"
     CDS             1103..1354
                     /locus_tag="ACHKW5_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7512442.1"
                     /translation="MRSPSKIVAVTPAAIEIEKRAIEREKKGQFRIAAHLWLQCMDVA
                     SGDVERARIAVRRDQCITKGNGLRRGDYSGIGCCGVVYD"
     gene            1347..1997
                     /locus_tag="ACHKW5_003091"
     CDS             1347..1997
                     /locus_tag="ACHKW5_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO7512443.1"
                     /translation="MTKKYTLIYADPPWAYRDKAADGNRGAGFKYPVMSVLDICRLPV
                     WDLADENCLLAMWWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGH
                     MTRANSEDCLFAVKGKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDVSRIE
                     LFARQTSHGFDVWGNQCEDPAVQLHPGYALDIAGLTNAFSNAPLSPTDNQGRERAA"
     gene            1994..2188
                     /locus_tag="ACHKW5_003092"
     CDS             1994..2188
                     /locus_tag="ACHKW5_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7512444.1"
                     /translation="MNRASPADLRKCLETANMLAHSGIRFVPIPAVTDAEFATLSAIF
                     ADKIESLAAEAEMEENQQNY"
     gene            complement(2195..3442)
                     /locus_tag="ACHKW5_003093"
     CDS             complement(2195..3442)
                     /locus_tag="ACHKW5_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7512445.1"
                     /translation="MLTDTKLKNLKPQDKLYKVSDRDGLYVAVLTSGTVSFRYDYRIN
                     GRRETLVIGQYGRDGISLAEAREELIAAKKLLKAGQSPAAAKRDGIKKIRGAETFAVH
                     TDSYMKHVILADSTRAMKQAVIDRDILPVLGNKMMTEITTSMVRDLCDRIVERGGRAT
                     AVQAREIISSVYRHANDRGHGLFNPAADIKPSSIAIFKPRERTLTPEEIGLFFRTLDA
                     IGAMGTMKMALKLVLITMVRKGEFTNATWDEIDFKKWTWTIPSGRMKGSRAHVIYLPK
                     QAQDILVGLQMCAGGSEYLVPGRYNFRKPLSNAALNSLIDRTVKIINEDGEHIQDFTV
                     HDMRRTASTLLHEAGYPSDWIEKALAHEQKGVRAVYNKAEYARQRAYMLQQWADMIDS
                     WIDGEHTDLIPFSPSKFEKWMAG"
     gene            complement(3635..5212)
                     /gene="guaA"
                     /locus_tag="ACHKW5_003094"
     CDS             complement(3635..5212)
                     /gene="guaA"
                     /locus_tag="ACHKW5_003094"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO7512446.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(5281..6747)
                     /gene="guaB"
                     /locus_tag="ACHKW5_003095"
     CDS             complement(5281..6747)
                     /gene="guaB"
                     /locus_tag="ACHKW5_003095"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO7512447.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            6909..8279
                     /gene="xseA"
                     /locus_tag="ACHKW5_003096"
     CDS             6909..8279
                     /gene="xseA"
                     /locus_tag="ACHKW5_003096"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO7512448.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(8276..8491)
                     /gene="yfgJ"
                     /locus_tag="ACHKW5_003097"
     CDS             complement(8276..8491)
                     /gene="yfgJ"
                     /locus_tag="ACHKW5_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO7512449.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(8560..10032)
                     /gene="der"
                     /locus_tag="ACHKW5_003098"
     CDS             complement(8560..10032)
                     /gene="der"
                     /locus_tag="ACHKW5_003098"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO7512450.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(10150..11328)
                     /gene="bamB"
                     /locus_tag="ACHKW5_003099"
     CDS             complement(10150..11328)
                     /gene="bamB"
                     /locus_tag="ACHKW5_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO7512451.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(11339..11959)
                     /gene="yfgM"
                     /locus_tag="ACHKW5_003100"
     CDS             complement(11339..11959)
                     /gene="yfgM"
                     /locus_tag="ACHKW5_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO7512452.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(11977..13251)
                     /gene="hisS"
                     /locus_tag="ACHKW5_003101"
     CDS             complement(11977..13251)
                     /gene="hisS"
                     /locus_tag="ACHKW5_003101"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO7512453.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(13362..14480)
                     /gene="ispG"
                     /locus_tag="ACHKW5_003102"
                     /gene_synonym="gcpE"
     CDS             complement(13362..14480)
                     /gene="ispG"
                     /locus_tag="ACHKW5_003102"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO7512454.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(14507..15520)
                     /gene="rodZ"
                     /locus_tag="ACHKW5_003103"
     CDS             complement(14507..15520)
                     /gene="rodZ"
                     /locus_tag="ACHKW5_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO7512455.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(15805..16959)
                     /gene="rlmN"
                     /locus_tag="ACHKW5_003104"
     CDS             complement(15805..16959)
                     /gene="rlmN"
                     /locus_tag="ACHKW5_003104"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO7512456.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(17109..17540)
                     /gene="ndk"
                     /locus_tag="ACHKW5_003105"
     CDS             complement(17109..17540)
                     /gene="ndk"
                     /locus_tag="ACHKW5_003105"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO7512457.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(17689..20001)
                     /gene="pbpC"
                     /locus_tag="ACHKW5_003106"
     CDS             complement(17689..20001)
                     /gene="pbpC"
                     /locus_tag="ACHKW5_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO7512458.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPDQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(20002..24963)
                     /gene="yfhM"
                     /locus_tag="ACHKW5_003107"
     CDS             complement(20002..24963)
                     /gene="yfhM"
                     /locus_tag="ACHKW5_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO7512459.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAN
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLYLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            25170..26015
                     /gene="sseA"
                     /locus_tag="ACHKW5_003108"
     CDS             25170..26015
                     /gene="sseA"
                     /locus_tag="ACHKW5_003108"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO7512460.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
BASE COUNT         6256 a         7180 c         6522 g         6232 t
ORIGIN      
        1 ttgaccttgc ccgccgctct gggcagatcc tgagtatctc ggctcgtacc gtacatacaa
       61 acgacaaatt cagctactca tacggcctag aagaaacgct cgagcattta ccttgcgaaa
      121 caggtgaccg cggagaatta acgcacgttt acgccgttgc acgactgaaa gatggcggag
      181 tccaatttga agttatgagc ctggcagacg ttgagaaagt tcgtgcactg agcaaagccg
      241 gtagcagtgg cccatggatt gatcacttcg atgagatggc taaaaaaaca gtaattcgcc
      301 gactgttcaa atatcttcct gtttctattg aaatgcagaa ggctgttgtt atggatgagc
      361 gcgctgaagc tggacttagc caagataacg cagctgttat cactggtgaa tattccgtag
      421 ttgacgatga gcgtcaacac ctgtcgccaa tttcagattc agaacgagaa gaagctcgag
      481 aatatatcat cgcgatactt aatagcctgg atccatctgc tgaagatgca aaaacgatgt
      541 tcaagcgcgc tgaaaatgag attaacacca tggctgaaaa gctcggtgat gaatatcacc
      601 aaaaattcat gatgacgctt aacgatatgc gtccagaatt cgagtaacca ccaccgcggc
      661 gccacgcacg ccgcactgca accaagagag gtatttatga aaggtgcatt aggtaagaag
      721 gaactcctgg cggtggtgcc actgtcatgg agcactatcg accgtatgga gcgcgcaggg
      781 gaatttccta aacgctggta tatcactgac aaacgctgcg catggaaccg tgacgaagtt
      841 gagcgttggc ttgatgaacg tcaggcagca agcccggcag agttccaggg taaaaagcct
      901 cctgttcagc aacgtgtata tcgtcctgtg agcaacgctg catgagtgcg ctgctaaggc
      961 actggagcaa atggtcagga tggtacttat tcctggcctc tgtttcagca tggctttatc
     1021 tgctggcatt aattttcaga gagggttgga ttaagtgaga aagttaagcc gacttgaaaa
     1081 atatcacatg aacaaggttt caatgcgcag tccgtcaaag attgtcgccg ttactcctgc
     1141 ggcgatagag atcgaaaaac gcgcgattga aagagagaaa aaagggcagt tccgcattgc
     1201 cgctcacctt tggcttcagt gtatggatgt tgcttctggt gatgttgagc gtgcaaggat
     1261 cgcggttcgc agggaccaat gtatcacaaa aggtaacggc cttcgccgtg gcgactatag
     1321 cggcatagga tgttgtgggg tggtttatga ctaagaaata cacactaatc tatgcagatc
     1381 caccctgggc ataccgggac aaagccgcag atggtaatcg cggtgccggt tttaaatatc
     1441 cagttatgag tgtgctggat atctgccgcc ttcctgtgtg ggatttggcc gatgaaaact
     1501 gtctgttggc catgtggtgg gtgccaacac aaccactcga agcactaaaa gttgttgaag
     1561 cctggggatt ccgtctgatg accatgaagg gattcacgtg gataaaatgt ggtagtcgac
     1621 aaccagataa actggttatg ggtatgggtc acatgactcg cgccaatagt gaagattgcc
     1681 tgtttgcagt aaagggaaaa ctacctacgc gcattaatgc agggatcgtt cagtcattta
     1741 ccgcaccgcg gcttgagcat tcaagaaaac cagatattgt tcgtgaaaaa cttgtgcaat
     1801 tgttaggcga tgtttctcgc attgaactat tcgcccgcca gacgtctcat ggcttcgatg
     1861 tttggggtaa tcagtgcgaa gacccggcag tgcaactaca ccctggatac gcgttggata
     1921 ttgccggatt aacaaatgca ttcagcaatg ctccgctgtc accaacagac aaccaggggc
     1981 gggagcgtgc agcatgaaca gggcatcacc agcagattta aggaaatgcc ttgaaactgc
     2041 aaacatgctt gcacacagcg ggatcaggtt tgttccaatt cccgctgtca ctgatgctga
     2101 atttgcaacg ctgtcagcaa tattcgcaga caaaattgaa tcactggcag cagaagccga
     2161 gatggaagaa aatcagcaga actattaaac gttattatcc cgccatccac ttctcaaact
     2221 tcgacgggga gaacggaatc agatccgtat gctccccgtc aatccaggaa tcaatcatat
     2281 cggcccactg ctgcaacatg taggcgcgct gtctggcgta ttccgctttg ttatatacgg
     2341 cgcgcacacc tttctgctca tgtgccagag ccttttcaat ccagtctgaa ggataaccag
     2401 cctcatgcaa caacgtactg gctgtacggc gcatatcatg tacagtgaag tcctgaatat
     2461 gctcaccatc ttcatttatt attttcaccg ttctgtcgat cagagagttc agcgcggcat
     2521 tagataatgg tttccggaaa ttgtaacgac caggaaccag atattcactt ccaccagcgc
     2581 acatctgcaa cccgaccaat atatcctgtg cctgtttagg caggtaaata acgtgcgccc
     2641 ggcttccctt catgcggcct gaaggaattg tccatgtcca ttttttaaaa tctatttcgt
     2701 cccacgttgc attggtgaat tcgcctttac gaaccatagt gataagcacc agttttaaag
     2761 ccattttcat agtgcccata gcaccaatgg catccagcgt gcggaagaaa agaccaattt
     2821 cttctggtgt cagcgttcgc tctcgtggtt taaatatggc gatagacgaa ggcttaatat
     2881 cagccgcagg attaaacaaa ccatgaccgc ggtcattggc gtgacggtat acgctgctga
     2941 taatctccct ggcctgcact gctgttgccc gaccaccgcg ttcgacaatc cggtcacaca
     3001 aatcacgaac catcgatgtg gtaatttcag tcatcatttt gttgccaaga actggaagta
     3061 tgtcacggtc gatcaccgcc tgcttcattg cgcgggtact gtcagccagg atgacgtgtt
     3121 tcatataact gtcggtatgt accgcaaacg tctcggcacc acgaatcttt ttgataccgt
     3181 cacgtttagc cgcagccggt gactggcctg ctttaagcag cttctttgca gcaatcagtt
     3241 cttctcgcgc ttctgccagg ctgataccgt cacgcccata ctgcccgatt accagcgttt
     3301 cgcggcgacc gttgatacgg tagtcgtagc gaaacgagac cgtgcctgac gtaagcacag
     3361 ctacatacag cccgtcacga tcggagacct tgtacagttt gtcctgcggc ttgaggtttt
     3421 ttaattttgt atcggtaagc acaattcacc cgtatagaaa ccattttcat gacggtatga
     3481 gagtatacct ttaaggtaat accgtcacct gtaccgccga aaaatatggt gtagagtgaa
     3541 tagaaatgaa tacataaaaa caaaaaccct ctgtaaaaac agagggttga gttagtatcc
     3601 gaatagaaat gacttgctat gagttagctg ttaatcattc ccactcaatg gtagctggcg
     3661 gcttgccgct gatgtcatac accacgcggg aaataccgtt cacttcattg ataatgcggt
     3721 tggaaacgcg accgaggaaa tcgtacggca gatgcgccca gtgtgcggtc ataaagtcga
     3781 tggtttcgac agcacgcaga gagacaaccc agtcatactt acgaccatcg cccattacgc
     3841 caacggaacg taccggcagg aacacagtga acgcctggct gactttgtcg tacaggtccg
     3901 ctttacgcag ttcttcaatg aagatggcgt cagcacggcg cagcaggtca cagtactctt
     3961 tcttcacttc acccagcaca cgaacgccaa ggcctggtcc cgggaacggg tgacggtaca
     4021 gcatgtcgta cggcaggccc agctccagac caatcttacg cacttcgtct ttgaacagct
     4081 ctttcagcgg ttcaaccagg cccatcttca tctctttcgg caggccgccc acgttgtggt
     4141 gagatttgat gacgtgtgct ttaccggttg cagaagccgc agattcgata acgtcagggt
     4201 agatggtgcc ctgcgccagc cacttcacgt cttccagttt cagcgcttct tcatcgaata
     4261 cttcaacgaa aacgcgcccg atgattttac gttttgcttc cggatcgttt tcgccagcca
     4321 gcgctgacag gaagcgatct tctgccggta cgtgaacaat gttaagacca aagtgatcgc
     4381 caaacatatc cagaacctgc tctgcttcgt tgagacgcag caggccgttg tcgacgaata
     4441 cgcaagtcag gtttttaccg atagcgcggt gcagcagcat tgcggttacg gaggaatcca
     4501 caccaccaga gaggccgagg atgactttat cgtcgcctac ctgctcgcgg atgcgagcta
     4561 cagcatcgtc gataattttc gctggcgtcc acagggcttc acactggcag atatcacgca
     4621 caaaacgctc cagcatgcgc ataccctggc gggtgtgagt cacttccggg tggaactgta
     4681 cgccatagaa gcgtttttct tcgttagcca taatggcaaa cgggcagctt tcggtgctgg
     4741 ctacggtgac gaagtcggac gggatagcgg taactttatc gccgtggctc atccagacat
     4801 cgagcagcgg tttaccgtct gcggtcagcg catcttcgat accgcgaacc agtgcgctgt
     4861 cgtttacgac ttcaacctgc gcgtagccaa attcacgttc gttagaggct tcaacgtgac
     4921 cgcccaactg cattgccatg gtctgcatgc catagcaaac gccgaatacc ggtacgcctg
     4981 cttcaaagac atactgcggc gcacgcgggc tgttttcttc ggtggtgctt tccgggccgc
     5041 cggaaagaat aatgccgctt ggattgaagt cacgaatttg tgcttctgtc acatcccacg
     5101 cccacagttc gcagtaaaca cccagctcac gcacgcggcg cgcaaccagt tgagtgtact
     5161 gagaaccgaa gtccagaatg aggatgcgat gcttatgaat gttttccgtc attgacgctt
     5221 attccgaggc aagtgaaaca gataatataa atcgcccgac atgaagtcgg gcgaagagaa
     5281 tcaggagccc agacggtagt tcggggactc tttagtaatg gtcacgtcgt gaacgtggct
     5341 ttcctgaatg cccgcaccgc tgatacgtac aaactccgct ttagtacgca gttcgtcgat
     5401 agtaccacag ccggtcagac ccatacagga gcgcaggccg cccatctgct ggtgaatgat
     5461 ctctttcagg cgacctttat aagctacgcg accttcgata ccttccggca ccagtttgtc
     5521 ggcagcgtta tcgctctgga aataacggtc agaggaacct ttggacatcg cgcccaggga
     5581 acccatacca cggtaagatt tgtaagaacg gccctggtag agttcgattt cacccggaga
     5641 ttcttcagta cccgccagca tggaacctac catcaccgcg cttgcgccag ctgcgatagc
     5701 tttggcgatg tcgccggaga agcgaatacc accatctgcg ataaccggaa taccggtgcc
     5761 ttccagggct tcaactgcgt cagcaacggc agtgatctgc ggaacaccta cgccagtaac
     5821 gatacgagtc gtacagatag aaccagggcc gataccgact ttaaccgcgc tgcaaccagc
     5881 ttctgccaaa gcgcgtgcac ctgcagctgt tgccacgttg ccgccgatga tttgcagatc
     5941 cggatattta gcacgggttt cacggatacg ctgcaaaact ccttctgagt gaccgtggga
     6001 ggagtcgatc agcagaacgt caacgccagc ggcaaccagc gcgtcaacac gctcttcgtt
     6061 acccgcacct gcgccaactg cagcaccaac acgcagacgg ccttgctcgt ctttacaggc
     6121 gttcggttta cgttccgctt tctggaagtc tttcacggtg atcatgccga tcaggtggaa
     6181 ttcgtcatca accaccagcg ctttttcaac gcgtttttcg tgcatttttg ccagcaccac
     6241 ttcacgggct tcaccttcac gcacggtgac cagacgctct ttcggcgtca tgtaaacgct
     6301 aaccggctgg ttcaggtcgg taacaaaacg cacgtcacga ccggtgataa tacccaccag
     6361 ttcgttttct tcggtaacga ccggatagcc cgcaaaaccg ttacgctcgg tcagttcttt
     6421 cacttcgcgc agcgtcgtgg ttggcagaac agtctgcgga tcagtcacca caccagattc
     6481 gtgttttttc acacggcgaa cttcttctgc ctggcgttca atggacatgt ttttgtggat
     6541 aaagccgata ccgccttcct gagccagagc aatagccagg cgcgcttccg ttacggtatc
     6601 cattgctgcg gaaagcatag ggatattcag acgaatagtt ttcgtcagct gggtgctgag
     6661 gtcagcagta ttcggcagaa cggtagaatg agcaggaacg aggagaacgt cgtcaaacgt
     6721 cagagcttct ttagcgatac gtagcatggg caatatctcg accagagtgg ttaataaata
     6781 ttgccgcggc attatacaga gcgtaaccga ttgcatctac ccctttttgc aaaaaatgct
     6841 tgctatcccc gaagggcggg ttactatcga ctgaataacc tgctgatttt gaatttgatc
     6901 tcgctcacat gttaccttct caatcccctg caatttttac cgttagtcgc ctgaatcaaa
     6961 cggttcgtct gctgcttgag catgagatgg gacaggtttg gatcagcggc gaaatctcta
     7021 atttcacaca accggcttcc ggtcactggt actttacact caaagacgac accgcccagg
     7081 tacgctgcgc gatgttccgc aacagcaacc gtcgggtgac cttccgtcca cagcatgggc
     7141 aacaagtttt agttcgcgcc aatattacgc tctacgagcc gcgcggcgac taccagataa
     7201 tcgttgagag tatgcagccg gccggtgaag ggctgctgca acagaagtac gaacagctca
     7261 aagcgaagtt gcaggctgaa ggattgttcg atctgcaata caaaaaacca cttccctccc
     7321 ctgcgcattg cgttggtgtg atcacctcaa aaaccggtgc tgcgctacat gatattttgc
     7381 atgtgttaaa acgtcgcgat ccttctctac cggtgatcat ctaccccacc gccgttcagg
     7441 gcgatgacgc gccggggcaa actgttcgcg ccattgaact ggcgaatcag cgcaatgagt
     7501 gcgacgtgtt gatcgttggg cgcggcggcg gttcgctgga agatttatgg agttttaacg
     7561 acgaacgcgt agcgcgggcg atttttgcca gccgcattcc ggtcgtcagc gccgtcgggc
     7621 atgagacgga tgtgaccatt gccgattttg ttgccgatct gcgtgcgcca acgccgtctg
     7681 ccgccgctga agtagtgagc cgtaatcagc aagagttact gcgccaggtg caatcgaccc
     7741 gtcaacggct ggagatggcg atggattatt atctcgccaa ccgcacgcgt cgctttacgc
     7801 agatccatca ccgattacag caacagcatc cgcagctccg gctggcacgc cagcaaacca
     7861 tgcttgagcg cctgcaaaag cggatgagct ttgcgctgga aaatcaactt aagcgtaccg
     7921 ggcaacagca gcagcggtta acacagcggc tgaatcagca aaatccacag ccgaagattc
     7981 atcgcgcgca aacgcgcatt cagcaactgg aatatcgttt agcagaaacc ctgcgcgtac
     8041 agcttagcgc cacgcgtgaa cgtttcggta atgcagtaac gcacctcgaa gccgtaagcc
     8101 cactgtcaac gctggcgcgt ggatacagcg ttactactgc tactgacggc aatgtactga
     8161 aaaaagtgaa gcaagttaaa gcgggtgaaa tgctaaccac acgtctggaa gacggctgga
     8221 tagaaagtga agtaaaaaac atccagccgg taaaaaaatc gcgtaaaaag gtgcattaag
     8281 ccagcacgaa ttcaacgcgt tttttcgata tcaagccatg cccgtgctga cagaagtaat
     8341 ctactgcacc gcaggctttt aacacctgca acggttgatg gcagtctgga caaagagctt
     8401 tcatttcaat aatttccccg caggatgggc aacgcgcatg gccgttatcc tgatcaagca
     8461 catgctgaca ttgtggacaa tgcagttcca tatgaattcc tccggtagcc atccctctac
     8521 attcatagag ggaatggcag ataaaatact tacggataat tatttatttt tcttgatgtg
     8581 cttcatcaga cgcttacgtt tacgcatctg ggttggcgtc agggtgttac gcttgttcgc
     8641 atacgggttt tccccttctt tgaactgaat acgaatcggc gatcccatta cgtccagcga
     8701 tttgcggaag tagttcatca agtagcgctt gtaggaatca ggcaggtctt tcacctgatt
     8761 accgtgaatc accacaatcg gcgggttata accaccagcg tgggcatatt tcagcttcac
     8821 acgacgaccg cgtaccagcg gcggttggtg atcttcaaca gccatcgtca tgatgcgcgt
     8881 cagcatagag gtccccacac gacgggtgga gctgtcatac gcttcacgta ctgattcaaa
     8941 caagttacca acaccactgc cgtgcaaggc agagataaag tgcacacgag caaaatcgat
     9001 aaagcccaga cggaagtcca gcgtttcttt cacctgctct ttcacttcct ggctcaggcc
     9061 atcccactta ttcaccacaa tgacaagtga acgcccacta ttgagaataa agcccagcag
     9121 cgagagatcc tgatcggaaa taccttcgcg cgcatcaatc actaacatca ccacgttggc
     9181 atcttcaatg gcctgcaacg ttttgattac ggagaatttc tctacagcat cggtgatttt
     9241 gccgcgttta cgtacgccag cggtgtcaat gagcacatac tcacgtccat cgcgttccat
     9301 tggaatgtag atactgtcac gcgtcgtgcc aggcatgtca taaacaacaa cgcgctcttc
     9361 accaagaata cggttagtga gtgtagactt acctacgttc ggacgaccca caatcgccag
     9421 tttgatcggc agactttgcg ggtcgaagtc gtcttcctct tcttcttcgc cgttctcttc
     9481 cgcttcaaat tgcgcccagt attcagcgtc ttcgtcgact tcctcttgcg gtgcgagatc
     9541 ttccatccac ggcagcagca catgctccag cagacttaat acgccacgac cgtgagacgc
     9601 ggcgatcggg tagatttcac ctaaaccaag cgagtagaaa tcaaccactg cctgatcggg
     9661 atccagaccg tcagttttgt ttgccaccag gaaggtcggt ttttcacggg agcgcagatg
     9721 tttggcaatc gcttcatctg ccggcatcag gcccgcgcgc gcatccacca taaacagtac
     9781 gacgtccgct tcttcaatcg ccagcagcga ctgttccgcc atgcgggttt ctacaccgtc
     9841 ttctgtgcca tcaatcccgc cggtatcaat acagataaac tcacggcctt caatttccgc
     9901 acgaccgtac ttacggtcac gagtcagacc cgggaaatcc gcaaccagcg catctcgggt
     9961 gcgagttaga cggttaaata acgtggattt tcctacgtta gggcgcccga caagcgcgac
    10021 cacaggtacc atgtttaaag cctcattttt ataaatcatc agacaacgca cgctatattc
    10081 gcgtcgttgt taaaaacagg aaaacggccc ctgtccagga gccgttttca aagtgaacga
    10141 cagagacgat taacgtgtaa tagagtacac ggttccgtct tttgcctgga tcagcagttt
    10201 gccgtcagcg gcaaccggtt cagtctggaa accggaacta tcaacttttt gctgggcaac
    10261 gaaacgacca tcttcgacgt taatccagtg cagataacct tcactgtcac cgaccaccag
    10321 gttgccatta tacagcaccg gagaagtcag caggcgatgc agcagatcgc tttgtgccca
    10381 cagcgtaacg ccgccatcaa tggtcaacgc catcacccgg tcattttgat cgaccagata
    10441 gatgcgattg ccgtcgacga tgaaatcatt caccgaaccc agttcgcgtt tccacataat
    10501 ctgaccactg cgcagatcaa gcgccgtcag gttaccatta taggccagcg cgaaaacaac
    10561 gccgttaaca acgacgggag tcgtgtcaac atcgctcaga cggtcaattt cggtagaacc
    10621 ggtcgcctgg gaaatacgct gctgccaaat catctggccc tgttccatca gcactgcgct
    10681 gacgcgacca ttatcgcccc ccacgacggc cgcaccaaaa gccgttgccg gcgcagactc
    10741 gccacgcaaa gagagcgaag gcatatcgag gttaactgtc catttgacag cgccgtcagc
    10801 ttcgttcagc gcttgtaact gaccgttact ggtgtggatt aacaccagac cgtcgctgac
    10861 caccgggcgc gaaagtgctt cacccgcgac tttagtttgc catgccacag taccatcgct
    10921 ggtattcagc gcgtaaacct gcgccttttc gctgccaatg tagacatgcc caccagacac
    10981 ggtcacaccg ccagaaagta atgcaggctc tttagagaac cagccatctt tctcggccag
    11041 gctgacagac cagatttctt tgccatcatc cgcattcagc gcttttacca aaccagcgcg
    11101 gtccgctgca tagacaacgt tgtccgccag tgccggatga agattggaat agaagttgcc
    11161 aatgccgcta ccaacggaag tactccacgc cgtggtcggc gtaaactggt tttcaacggt
    11221 tggcaatggg gacatcttta ccacatcttc ttcgctgtta aacagcgaac agccgcttaa
    11281 aagggtaacg gaaagcagtc ctggcagcag taatttacgc aattgcatcg ggtccctctc
    11341 agatggacaa attattaatt ttcatctgca tcatttcgct cagtgccggt gtaacatcgc
    11401 ttttcacgcc tgcttcccat gcactacgcg caccttgctt atcacctttg ctcagcaatg
    11461 cttcaccacg caggtcggca acaatggcag cccacccttc acctttgatg gtatcaaggg
    11521 ttttcagcgc ggcatcagcc tgcttgagct gtacctgaac gcgagcaaga cgcagattta
    11581 tcacggcttt gagattttca tcgctcgtgt ctgccagccc ctgttgtaac tgggcggcag
    11641 ctttctctag ttcatttttg tcaacaaatt gctgcgcaag ttccaaagaa gccagcgcac
    11701 cataagtatt tttattttca gcagcaaatt tttccgccgc cgggatgcta tccggtttgc
    11761 cttcgctcac tgcggtaacc gcattttgat aggcaagaga agcggagcgt gcagaatcaa
    11821 cctgatggct gttccagtag cgccagccaa tcagtgcgcc aacgcccaaa atcaccccaa
    11881 cagccagtgc tttgccattt tcagcaaaaa agcgtttaac cgcttctacc tggtcgtttt
    11941 cgttctcgta aatttccacg ctgtccttct ccttccttaa cccagtaacg tgcgcaagtg
    12001 cgcggctacg ctatcctgcg caactgccgt ttgctcacca gagcgcaaat ccttcactac
    12061 tgctgtgccg ttagccactt cagactcacc cagcaccaca gcaacgcggg caccccattt
    12121 atcagcacgg gcaaactgtt tcttaaagtt gccgccgccg tggttggtca tcaatttcac
    12181 gcccggtaat tcatcacgca gacgctcagc taatgccata gccgcagatt gtgtatcagc
    12241 acctgaagcc accaggtata tatcgacaac aggatcggct ttaaattccg gattaacggc
    12301 ctgtactaac aatacaagac gttcgaggcc catcgcaaaa ccgacagccg gtgttgcacg
    12361 accgcccagt tgttccacaa gaccgtcata acgaccgcct gcacacacgg tgccctggga
    12421 gccgagactg ttagtcaccc actcgaaaac ggtacggtta tagtaatcca gaccacgcac
    12481 cagacgctgg tttacggtgt aagcgatccc cgcgctctca agcagtttgc acagaccggc
    12541 aaaatgctca cgagattcct cgtccagata atcacctaat gccggagcgt cgttgagaag
    12601 cgcctgcact tccggatttt ttgaatccag cacgcgcagc gggttagtgt acatgcggcg
    12661 tttgcagtct tcgtccagct tttctttatg ctgctcaagg aatgccacca gcgcatcgcg
    12721 gtaattggcg cgtgcttcca gcgaaccgat agaattcagc tcaagagtta cgtgttcgga
    12781 aatacccagc gcgcgccacc agcgggcggt gagcataatc agttcagcgt cgatatccgg
    12841 accttgcaga ccgaaaactt cgcagcccaa ctgatggaac tgacgataac gccctttctg
    12901 cggacgctcg tgacggaaca tcggcccgat ataccacaga cgctgttcct gattgtacag
    12961 aagaccatgc tcgatgccgg cgcgtacaca gcccgccgtc ccttcagggc gcagagtcag
    13021 gctgtcgcca ttgcgatcct caaaggtgta catctctttt tcaaccacgt cggtgacttc
    13081 accaatcgca cgtttgaata gcggggtctg ctctacaatc ggcaagcgga tttcactgta
    13141 accgtagctg ccgagcacgt ttttcagtgt gccttcaatg cgctgccaga tggccgtttc
    13201 gccaggcagg taatcgttca tgccgcgaat ggcttgaatg ttttttgcca cgtttattct
    13261 ctttctgaat ataaaaatga accctcaacg cttccctcaa tgtttcggga gctatgcggg
    13321 ttcaatcata cacgggaagc gaggcgcttc ccatcacgtt attatttttc aacctgctga
    13381 acgtcaattc gacgcgcttc gtccagctga ctggctttcg cacgaatgcg tgcttccagc
    13441 tggtcgatca tatcgttgtt gtccagacgg tctttgcgca cgccatcttc atagaggccg
    13501 cttttcttgt tgccgccggt gacgccgagt gtagaaacca gcgcctcacc tgggccattc
    13561 accacgcagc cgataatcga aacgtccatc ggagtgatga tatcttccag gcgttgctcc
    13621 agcgcgttaa ccgtaccgat aacatcaaat tcctgacgcg aacaggtcgg gcaggcgatg
    13681 aagttgatcc cgcgcgaacg gatacgcagc gatttcaaaa tatcgaaacc gactttgatc
    13741 tcttcgaccg gatcggccgc cagtgatacg cgcagcgtgt cgccgatgcc ttcagacagc
    13801 agcagaccta aaccaatggc ggattttact gccccgctgc gtgcaccacc ggcttcggtg
    13861 atccccagat gcagcggctg atcgatctgt tttgccagca aacgataaga ctcaacagcg
    13921 aggaagacgt cagacgcttt cacgctgact ttgaactgat cgaagttcag gcgatcgaga
    13981 tgatcaacat ggcgcatggc ggattccagc aacgcctgcg gcgtcggttc gccatacttt
    14041 tcttgcagat ctttttccag cgatccggcg ttaacgccaa tacggatcgg aatgttttta
    14101 tcgcgcgcac agtcaaccac catgcgaata cgctcttcat taccgatatt gccagggtta
    14161 atacgcagac aatcgacgcc gtattccgct actttcagtg caatgcgata gtcgaagtgg
    14221 atgtcagcca ccagcggcac gttaacctgc tgtttgatga gtttgaacgc ttctgccgcg
    14281 tccatcgtcg gtacggagac acggacgata tcagcgccaa cgcgttccag cgccttgatt
    14341 tgattgaccg ttgcttcgac gtctgtcgta cgcgtattgg tcatggactg tacggcgatg
    14401 ggagcaccat cgccaatcgg cacattccca acgtaaatac gtgttgattt tctacgttga
    14461 attggagcct ggttatgcat gaaaaatctc ccgcgttacc cgtctgttac tgcgccggtg
    14521 attgttcggc attgagggtc agacgcgcaa cctggttagt tctgataaaa cgactcagat
    14581 cgacaggttt cccttgatac tggatctgta ctgcggctgg cgcaccaatt ttcagtttgt
    14641 atggtgcctg gccggttaag ttcaaattac cgtctttacg ctgcataccg ctaaacaatt
    14701 ttttaccggt agcatcagta acctccagcc agcaatcggc agtaaagttc atcaccagcg
    14761 cattcggatc agccgccggc gtagtcacgc ctgcctgatc ggttggcaac ggcgcagcgc
    14821 catctggcgt tgttgttgcc gttggtgccg gggtcgcggc ggtatcaaca tttgcctgcg
    14881 aaggcgaaac aaccgcattc tgttgcggat caacagctgg tgctggcgca gttacggcag
    14941 gtgtttgcgt gttggttgcg gtagtatcca cagacgctgg cggcgtggac gttgtagccg
    15001 ggtctgtagt tgtcgacgta tttaacggaa cactctgccc ctgctcgcta ttactgctca
    15061 gttccgccga agattgatcg gccatagtgg tgatctcttc ctgctgagct ttgtggtctt
    15121 gccaccacca ggcaccgctc aggccgataa ccacaaacaa caccagccag gtgaaggtca
    15181 tcagccagcc gtcacgtttt ttgcggcgtt taccgaggga aaaactctgc ataggcgcaa
    15241 cttttgcagc ccgtagtgga gcctgctttt ccagccctgg cagcagttct tcttctggaa
    15301 tatgtaccag acgcgcataa gagcggatat atccgcgcag gaatgttgaa gcaagatcgg
    15361 cgggtgcctt atcttcttca atgtcgcgta ccgtggaaac cttcaggcaa agtcgctcgg
    15421 caacggcctg ctgactaagt cctagttgtt cgcgagcatt acgcaggcga gcgccggtag
    15481 taagtgcttc attttggtcg tgcgtggctt cagtattcat tcgctgcagg tacgtttaaa
    15541 tgagaattga gatgccggtg aaccatcatg ctcacccaca ccgcgaaaca tccgttaagt
    15601 taaccgttat catacagtat aagaccgtct gtgtgctcgt gacaaagccc acattcacta
    15661 aggctaactt actgttgcat cgttacatac tgccttaaag tcagcaaaaa cgcaccgtta
    15721 atattgacca gacaattgca acttaatact tcattcataa aagattacgc cacggtacat
    15781 aaagtaaccg tggcgtaatg gctatcagac cgctttaatg tcgatggctt caccctgcat
    15841 ccgtttacgc agggtacgtt tcgtacggtc gataacatcg cccgccaact gaccacaggc
    15901 agcatcgata tcatcaccac gggttttacg cacaatagtg gtgaaaccgt agctcatcag
    15961 cacttttgag aaacggtcga tacggctgtt cgagctgcgt ccatacggcg cgcccgggaa
    16021 ggggttccac gggatcaggt tgatcttaca cggcgtatct ttcagcagtt ccgccagttg
    16081 gtgcgcgtgt tcagtgccgt cgttaacgtg gtcaagcatc acatactcaa tagtgacacg
    16141 gccctgattg gcgttggatt tctccagata acggcgcacc gcagcaagga acgtttcgat
    16201 attgtacttt ttgttgatcg gcacaatttc gtcacgaatt tcgtcgttcg gcgcgtgcag
    16261 ggaaattgcc agtgcaacgt cgatcatatc gcccagttta tccagcgccg gaactacacc
    16321 ggaagtagaa agcgtgacgc gacgtttaga caggccaaaa ccgaaatcat caagcatgat
    16381 ttccatcgcc ggaacgacgt tgttcaggtt gagcagcggc tcgcccatgc ccatcatcac
    16441 tacgttagtg attgggcgct gaccggtgac ttttgctgcg ccgacgattt tcgccgcacg
    16501 ccacacctgg ccaataattt ccgatacccg caggttgcgg ttaaagccct gctgggcggt
    16561 ggaacagaat ttacactcca gcgcacaccc cacctgcgaa gagacgcaga gcgtggcacg
    16621 gtcgtcttcc gggatataca ccgtttcgac gcgctgatcg ccaacggcga tcgcccattt
    16681 aatggtgccg tcggatgaac gctgttcttc aaccacttcc ggtgcgcgga tttccgccac
    16741 ctctttcagt ttgccgcgca acactttgtt gatgtcggtc atctcatcaa agttgtcgca
    16801 gcaatagtga tacatccact tcatcacctg atcggcgcgg aagggttttt cacctaaatc
    16861 tttaaaaaac tcccgcatct gctgacggtt gagatccagc aggttgattt ttccatcttt
    16921 cgtggtgacg ttttcaggtg tgactaattg ttcagacata tgctattccg gcctcgttat
    16981 tacacgttat ggcccctgga ggattgaaaa aagaaacgcc ccggtgagcg gctgctcgtc
    17041 cgggggcgct gcattgtaca aattctggcg cacggatgcc acgtttgcac gcggcattta
    17101 cgaaattatt aacgggtgcg cgggcacact tcgccttcgc caaagaaata agcgatttcg
    17161 cgagcggcag attcgacgga atcagaaccg tgggtaccgt tttcggtcag gctgtcagcg
    17221 taatcagcgc gcagagtacc agccagtgcg tttgccggat tggtcgcgcc cagcagatcg
    17281 cggtgacgct gaacggcgtt ttcaccttcc agcacggaaa ccacgatcgg gccagaggtc
    17341 atgaattcaa ccagaccatc aaagaacggt tttccatcgt gttcagcata aaagccacgt
    17401 gcctgttcaa cggtcaggtg cagcattttg gtgccgacaa ttttgaaccc tgcagcttca
    17461 aagcgcgcaa agatattacc aatgacgttt tttgctaccg cgttcggttt gatgatggaa
    17521 aaagtacgtt caatagccat ttttacctct gtaaattgtt ctgttgttgt ctgtaccagc
    17581 gtacgagatg gcgcggatta taatgagcaa cagggccgtt gactattgat gaaggtaaca
    17641 tttttttaaa ataaaatgag ttttagcaac aatcagggac aaaacagact attgcatgac
    17701 aaatttcact gtcgcgattt gccccacatc atccatcacc agcaattgat aatcaccttt
    17761 atccgtcaaa tgcagggtca cgttgcgccc gcgttcagtt aacggttcgc cattcagaaa
    17821 ccaccagcgt tcacctgccc ctccacttga ttgcaacggc aaagtcgctt ccgctgcgcc
    17881 cggtaaacgt ttaataatcg cgccatcgcg aacgcctgtc agctgcagag ggagctgggc
    17941 atcgtgaccg tatgacgggc aaattgtcga ggccggtggt aagcgcacag cgcgacgttc
    18001 tgatgcgggc agccagggtt ccagcggtag cggccagaca tttatcattt cttgtcgcgc
    18061 ttgcgggcaa tcagcggcaa cacgtttgcc attttcatcc agccagatgg ggaaacgaat
    18121 gccattaatg ccttcctgat ccggcagtaa tagagttggc ggctgactcc cgtccagcag
    18181 ccaggttgcc aggcggcggc gacagttacc gtcaccttcc ggcaaagact gtccgcccgg
    18241 ccagcagata acgccacgag tgactgagtt cgggcgcggg tcttccggca gattcgcact
    18301 gcgcgacagt aagatattat tgacctgatt caacaatggt acggcactgg caaagccaaa
    18361 ctgaccaaca acgggcgtgc cgtccggtct gccagtccag atcccaatga catagcgagc
    18421 gttaacccca atcgcccagg cgtcacgata gccatagctg gtgcccgttt tccatgccag
    18481 tggggcgacg cgcggcaagg cactatccgg caagggtttc gcttcatcag ccataatccg
    18541 gcgaatgatc cacgccgccc ccgacgacat taaaggccgt tcaagcagcg gatcgtcagg
    18601 ctgtaagcgc aatttgcctg ccttgccgtg gcgagcaaac gcggtatacg ctgccgccat
    18661 atcttccagt tttgcaccag cgccgccgag aatgagtgaa agattcggcg cagcaccgtt
    18721 gggcaaatat aacggcaatc caacattgcg taactttgcc gcaaaccgtt tcggtccata
    18781 ggcttccagc acctgcacag caggtaagtt cagcgagcgc accagcgcct cgctcatgct
    18841 gatcgggcca tgaaaaccgc tatcaaagtt acctggtcga taatcaccgg tgcgccgggg
    18901 gacgtcttgc agcagtgatg ccgggtggat caagccttca tccagcgcca gaccataaac
    18961 aaacggtttg agcaccgatc ctggcgatcg gatcgcattg accatatcaa catgaccaaa
    19021 gcgtgaatca tcgttgagat caaccgatcc cacccagccg cgaacacgca tatcggtatg
    19081 atcaaccacg atcatcgcca gtgagctgcg cggtggcaac cgccctttcc agttttgcgc
    19141 cagttcttcc agacgtcgtt gaagaccggc atccagcgta gtaacgattt tgtcgctttt
    19201 gcttttaccg agcatcatgc gcgaaaacag cggtgccagt tgcggcattt gtcggggtgc
    19261 cagccagatg ggttcttccc ttgactcttt tacctgctca cgggaccaca caccttgcac
    19321 ggccatccgt tcgagcactt tattacgcgc ggcttcggca cgctccggcc aacgatccgg
    19381 gcgaagacgg ctgggcgctt gcggcaaaac cgccagcatt gccgcctcgg aatagcttaa
    19441 attcgcaggc gactttccga gataagccca acttgccgca ccgatcccct gcaacgtacc
    19501 gccaaacgga gcgcggttaa gatacaaggt cagaatttca cgcttagaca gatgccattc
    19561 cagttgcaac gcgcgccaga gctggcgaat tttgccgcca aatgttttgg ggtgaggatc
    19621 aagcagacga gcaacctgca tagtgagcgt gctgccaccg gaaataaccc gtcccgaagt
    19681 gagatcttgc catgctgcgc gcgccaccga gaatggattc acccccggat gcttccagaa
    19741 ccagcgatct tcataattga tcagcgcttc aaggtaacgt ggagaaacat cttcgattgt
    19801 taccggataa cgccagatgc cgtcagcatc ggcgaagcgc catagcggcg taccatcctg
    19861 cgccacgacc actcgtgcgg gattgacttc atgcagcggt agaggccaga gtttatctgc
    19921 tccccacgct gcaagaaaaa ggagaaatgg cgcagctgcc agcgttatcc agcagccgcg
    19981 tttggttaac aagcgaggca tttacggtcg gacaatcagc agatcttcag ccgcgccggt
    20041 cgcccgccat tggggaacat acattgattc caccatcggt tgcggtacct gatacgttcc
    20101 cggcgtcacc gcccgcgcca gatacaccag cgttaccggt tggtattcat caacggcaac
    20161 cgccgccaca aagcgatcgt cacggaactc aatgtgctta atactcgcct gctgcatctg
    20221 gttcagtaag ttttgcactt cgccaccact ttgctccagg ctggcgctac cgttcgccag
    20281 attctgattt tccagttcca gacccgcagg cagcagatcc acgactaacg catccggcac
    20341 gctgttactg gcttttacct gcaaccagac cagcaccaga tcgccgctac gtaacgagtc
    20401 cagcgatttg ctcttaccat cagtaccaag aatatgacgc tcgatttgca gcacattgtt
    20461 cgccggtaaa ggtgcggatt gcggataacc gctggcatcc acacgcagcc ataacggctg
    20521 atcgccactg tttctcacct gcaaggtggc aagttgatcg ctattcagat tgctgtttag
    20581 cgttttctcg cctgtcagcg gctcagctga gaaagaggtt tgcgcctgcc atttacccgg
    20641 taaatcctga atcgtacggg cagccaggaa caacgcgtta ctttcctgcg tcgatagcca
    20701 gcgttcacca aatgcctgct gcgaaagcgt gttcagcaaa gtgttctgct catcgggtag
    20761 cagtttattt tcttccagca agtagagcat taacgcgttg tcgcgcagtg gactaccgta
    20821 atcacccagc catatccgct catcactatt tctcggcgtt ttcagcgcca gcacaatcgc
    20881 ttcttcacca cgcgttgcat cacccatggt tttcagcgca acgccaagtt gcagcagcgg
    20941 taaaccagaa gcggcatctg cacgatgctc ccagatttca cgcagcgcac ccagtggtgc
    21001 tttttgctga cgggccagca ccagcgcagc gtaagactga acggcgaatt tactggcttt
    21061 gagattatcc gcgtacggga tcgacatcat gcccggatct tgtaaatagc gcagcagacg
    21121 ctcattaccc cggttaatgg cgtctgtcgg cacgctgtaa ccctgttcgc ctgcgcggac
    21181 caggaaatcc atcacgtaag ccgtcagcca gtactcttcg tcaccgtttt tatcccacag
    21241 cgcaaagccg ccgttatcac gttgcatttg cagcaaacgg gaaatgccga tatcgaccga
    21301 tgcacggcgt ttctcatcac tgtcgccttt gatgcccaac gcctgcagtt gggctgcgtt
    21361 ggtataaagt gacgggaaca ggccgctggc ggtttgctca agacagccgt acggatacgc
    21421 ttttaactct ttgatataac gtgcaatgtt cagcggtggt ttgccgctca acaacaattg
    21481 cccttccagc gtaacaggcg agaagttttg caatccatcc gccgggttcg cccatgtctc
    21541 accaggctgt aacgccgtac cgtaattaac cgtttgtgcc gggaacgccg gacggacgcc
    21601 gattttccac tgcttatgct gatcggcaac ggtttcaccc ggtaacgcta acccgctaat
    21661 ggtggcctga atttctccat cgccataacc cggcaatgct cgcaccggga taaacagcgt
    21721 agtacgcaca cctggcgcta attcaacggc tgcgggtgaa tcgctgacca gttcaagcaa
    21781 accactggcg gtcagggcaa cgttcagttt ttgcggttta tcggtaagat tagtgatatc
    21841 cagcgtcaga cgcgaggtat cgccactcgc cataaagcgc ggcatgttca gttcagcaat
    21901 caccggtgcg gcaactatca ctttgctttc gttgctaccg aaatcatctg ccgtccaggc
    21961 ttgcgccatg acgcgcaatt caccgttaaa atcgccaatc ggcagtgtaa ccgagccttc
    22021 gccctgttcg ttgagcgtta ccggcagcgc ctgctgcgcg acaatattga cgtgattgac
    22081 cggcggttta ccaccacgtt tcagctcatc accatcgcca ccgaaacgca gagctgccag
    22141 acgcccctga ccttcaataa cctgaccgta aatatcgtaa atgtctgcgc catagcgttt
    22201 ctgaccaaag aacgcctgcc acggatctgg cgtgacgtag tcagtaatat tcagcacacc
    22261 actatcaacg gcagacacca gcacattcac ctgtttaggc ttctcgccat ttttagtgct
    22321 ggctttaatt ttcacggtta atggttgatt gggacgcatt tttgctggtg tttccagcgc
    22381 cagatcgagg cgacggtttt catcgccaag cggcagatgc aacacaccaa ccgcgcgttt
    22441 tggcgtcgcg gagcgagatt tatcgccagg acgtaccacc agcgtactta aatacagatc
    22501 atgacgattc caggttttat cgaccggaat cgtcagatcc agcccttgag ccggaacatc
    22561 aatctcttgc caccacagcg gcccttcact ggactcgacc atcgcataac ctttacccgc
    22621 cgttggcgcg gcgatatgca acttaatggt gtcgccaggg cgataactgg ctttatccag
    22681 tttcagcgtg acacggtcgg gtcgcactgc gccgctaccg tcgctgttgt cctgccagct
    22741 atagccagcc cagaaacgaa cactactgac cgcttcattc ggcgctttga cttccagacg
    22801 ataagcgccc cactctaccg gaaaactgac cttgccggtt tcgtccgctt tcagatccag
    22861 agtttgttca ttttcgatca gatctttttg atcaaactgt gactgccagc cttcatcttc
    22921 tgaccagttc cagtagtaat cgcggcgttc gcgaatcagg cgcacctgca agcccgacac
    22981 ggcttttttc acgccttgcg catcgctata aacgatgtca aaagcggcgt tactgccttc
    23041 atcaacaatg ggctgtttca ccgtgctgtc agtacgataa tcgtaaaccg atttcgaggc
    23101 gaactgcgga cggatccccg gcaatgcatc ggcaggccag atagcctgct cagcgcggcg
    23161 cgtcaccggg cgaccgcccg attccagcaa actaccctgg aaaataacct gtaatgggga
    23221 atgcgtttcc ttccactggc tttctgtaga aacttcgccg cgccctttat catccagcgt
    23281 caactgaact tcatccagcg tgcgggaaag attttcggca gctatatcgc cgaattcaaa
    23341 accaggtaag gctgacacag cttcacgcag tgggcgcagg aaaagttgcc cttgcaaagt
    23401 attaccatta gcaggtgcac catacaggta gtaccccacc acggagaatt tcacttcatc
    23461 tttcggcgtt agcggggttt tctcaccggt cagattcagc gccatgcgct ctggcataaa
    23521 atcttcgacg tggaaatccc acatccgata ctggttatcg cccgtgttag cgcgaatatg
    23581 ccacataccg gttgccgcat tgctatcgag tggccaggta aagtggtaga ggccattctc
    23641 cggctgacta acgacgctcc tgagtacctg cccatcgggt ttaatcacgt ctaacttgat
    23701 gggttgattg ggcaacgctt taccgtctgc atcacgcagc aaaccattga ggattaccgt
    23761 ttcacctggg cgataaagat cgcgtgggcc aaacatgaaa aactgtttgc tatagcctgg
    23821 cgcgccagca atgttaaatt ctgctaagtc cagcgccgga agttttaaat cgagtagcgt
    23881 tgtctgaccg tctttacgcg ccaacagtaa tgccgcgttt ttatcatttt ccagctgcac
    23941 atgcccctga gcgtcactgg ttgcctgagt cagagtctgc cctttctcat ttaataaaga
    24001 gacttcaatt ccttgctggg ccgcgccgtt ttccagactt tgggtaaaga tatccagacg
    24061 attgtgataa cggtgagctg aaacgccgat atcacttaac gtaaacagcg tcgcgggatt
    24121 actgtaatcg taacgtccag cctgattcat cacagccaga tacacgcccg cctgctgaag
    24181 cggtttgata tcgcccagcg gcagcaataa tttttcacgg gtgttacgcg caggattgag
    24241 atcaaaccgt ccggtgtaga ccagatccgc catctgcagc agtttgtctg actgccagtt
    24301 cgccagcgaa ttgcggtatt cccattggct aatgaatgct ggcagagatt ctggcttaac
    24361 gcggaagaag ttaacatcga cattattaac gttgagcgcc attaccggca gcccttcaac
    24421 gactttgcca ggcagcagcg aaccacggct ggcaaaaccg acgctgggtt ggatgtcgcg
    24481 ggtagttata gttttttcgt aatctttact gaaggttgcg ttgttgagtg ctttgacctc
    24541 cttgccaata gtaacgatca aatcacgttt cggttcgagg tggcgtaaac gtaactcttt
    24601 aagattatct gacagctccc aggcaccatc cactttgccg ctttttttat cgacgacatg
    24661 aataacgcgt gagaaatcct gatccgggtc gagagggatg gagaacgtca gcaccagcgt
    24721 ggctgcacca tcaagttgga cttcagagag atccagcaat gtcagcacct taccggcact
    24781 ctgttgggcc agtttttgtc tctccggcac ggagagtttt gctgaactcg cgttttctga
    24841 agaggccgct ttagtaactt cagaaggcgc atcttttttc accgctgttg gcgcgttatc
    24901 gttgttgtcg caccctgcca gcgccagcat tagcatgcag gcggctacgc gtaacttttt
    24961 catttttcat ccctgccaca atggcccgtt agcaacgtcg aataattatt atgcgtgaga
    25021 atttacgtta tgtaattcag tatcacgccc atacaatccg aattttagtg agaattgtgg
    25081 ctcagacagc caaatcagcc tctttcgctt gtcgcttttc actaaacatc cgacaattta
    25141 gcctaacccc ggcaaaaatg gagatgccta tgtccacgac atggtttgta ggagccgact
    25201 ggctcgccga acatattgat gacccggaaa ttcagattat tgatgcccgc atggcgtcgc
    25261 ctggacagga agatcgtaac gttgctcagg agtatctgaa tggacatatt cccggcgcag
    25321 tgttttttga tatcgaagcg ctttctgatc acacttcccc gcttccgcac atgctgccgc
    25381 gcccggaaac gttcgccgtg gcgatgcgtg aattaggcgt taaccaggat aagcacctga
    25441 ttgtctatga cgaaggtaat cttttctcag ccccacgagc atggtggatg ctgcgcacct
    25501 ttggtgtaga gaaagtgtcg attctggggg gtggacttgc aggctggcag cgcgatgatc
    25561 tgctgttaga agaaggtgca gtagagctgc cggaaggaga gtttaacgcc gcgtttaatc
    25621 ctgaagccgt ggtgaaagta accgatgtat tattggcaag ccatgaaaat acggcgcaaa
    25681 ttattgatgc ccgcccggct gcacgtttta acgcagaagt tgatgaacct cgcccaggtt
    25741 tacgtcgcgg acatattccc ggagcactga atgttccgtg gacggaactg gtgcgcgaag
    25801 gcgaactaaa aacgaccgat gaactggatg cgatattttt tggtcgcggc gtcagctacg
    25861 acaaaccaat tatcgtcagc tgcggctctg gtgtaacggc agccgtggtt ttgttagcac
    25921 tcgcgacgct ggatgtgcca aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg
    25981 cgcgggcaga tttaccggtt gagccagtga aataagtatt ttacaggcaa taaaaaaccg
    26041 ccgaatttgg cggtttttta ttgctggtct ggttcgcggc ctttccagca ggttgacttg
    26101 tgttacatga gcaacgcagg tgcttcacag caaaacaata ctcaccagta actctctttt
    26161 tgtcaagcaa aagagagtaa ttattgttta
//
LOCUS       DAZFYC010000070        25082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFYC010000070 DAZFYC010000000
VERSION     DAZFYC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.070"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHKW5_003109"
     CDS             complement(60..341)
                     /gene="yehE"
                     /locus_tag="ACHKW5_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO7512461.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHKW5_003110"
     CDS             complement(604..1713)
                     /gene="apbC"
                     /locus_tag="ACHKW5_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO7512462.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIVSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPGLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1845..3878
                     /gene="metG"
                     /locus_tag="ACHKW5_003111"
     CDS             1845..3878
                     /gene="metG"
                     /locus_tag="ACHKW5_003111"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO7512463.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4019..7813
                     /locus_tag="ACHKW5_003112"
     CDS             4019..7813
                     /locus_tag="ACHKW5_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO7512464.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7823..11455
                     /gene="yehI"
                     /locus_tag="ACHKW5_003113"
     CDS             7823..11455
                     /gene="yehI"
                     /locus_tag="ACHKW5_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO7512465.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11516..11836
                     /gene="yehK"
                     /locus_tag="ACHKW5_003114"
     CDS             11516..11836
                     /gene="yehK"
                     /locus_tag="ACHKW5_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO7512466.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            13019..14107
                     /gene="yehL"
                     /locus_tag="ACHKW5_003115"
     CDS             13019..14107
                     /gene="yehL"
                     /locus_tag="ACHKW5_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO7512467.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYTLLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14118..16321
                     /gene="yehM"
                     /locus_tag="ACHKW5_003116"
                     /pseudo
     CDS             14118..16321
                     /gene="yehM"
                     /locus_tag="ACHKW5_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            16314..17450
                     /gene="yehP"
                     /locus_tag="ACHKW5_003117"
     CDS             16314..17450
                     /gene="yehP"
                     /locus_tag="ACHKW5_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7512468.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17447..19447
                     /gene="yehQ"
                     /locus_tag="ACHKW5_003118"
     CDS             17447..19447
                     /gene="yehQ"
                     /locus_tag="ACHKW5_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO7512469.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PAPACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19572..20033
                     /gene="yehR"
                     /locus_tag="ACHKW5_003119"
     CDS             19572..20033
                     /gene="yehR"
                     /locus_tag="ACHKW5_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO7512470.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(20074..20544)
                     /gene="yehS"
                     /locus_tag="ACHKW5_003120"
     CDS             complement(20074..20544)
                     /gene="yehS"
                     /locus_tag="ACHKW5_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO7512471.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(20591..21310)
                     /gene="btsR"
                     /locus_tag="ACHKW5_003121"
                     /gene_synonym="yehT"
     CDS             complement(20591..21310)
                     /gene="btsR"
                     /locus_tag="ACHKW5_003121"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO7512472.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(21307..22992)
                     /gene="btsS"
                     /locus_tag="ACHKW5_003122"
     CDS             complement(21307..22992)
                     /gene="btsS"
                     /locus_tag="ACHKW5_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO7512473.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            23214..23279
                     /locus_tag="ACHKW5_003123"
                     /pseudo
     CDS             23214..23279
                     /locus_tag="ACHKW5_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23608..24834
                     /locus_tag="ACHKW5_003124"
     CDS             23608..24834
                     /locus_tag="ACHKW5_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
                     /protein_id="HFO7512474.1"
                     /translation="MILIADMFAAKPELSQRCISGLLTTGPNDENVNNKPSSDMPNRI
                     KPVVSDSQIGIYTADSNTLKDGLCSQAHIGTQPYRPGADSPITVRSFLQITLSNNNYK
                     SLEHLQTRAENYLRYRKAEENILRSTVEGLTNPESPVFKQTAWMGHLERGLWKTETRW
                     DGNDREQLGKEALGSEEPKPGSPFYGSRGLKLSDSAHSAFSMMLCGSEGPFTKEQALS
                     GFELAQTGQVLAGRLKIQERVKFRADNRIDAQRNGTHSTRTPTGMDLSQDIGTIMRDK
                     AGLPVMSGTSGSSSDATLATRYAAEHFGKTWAAPGLSQAEGCKAISDLSHHYFRAEGS
                     SPPQSMATGINKVRYDAGMEEKYVNTLDIFTHSYPEIYAGVALTIAGAGGNDEQAMYN
                     VTQEAARILHEAETKD"
BASE COUNT         6439 a         6419 c         6217 g         6007 t
ORIGIN      
        1 gaaatcattt atataaatga tttcggcttt ctaaatgcaa ttaaaatcag gttgccaaat
       61 taatcgtagc gatttaacac gattcgacgt ttgctatcgc tacctgcaac aaccacttct
      121 gtcgttacac ctttgacggg cgaagaaaac agatgacggg tgtcaacatt atacgttaac
      181 tgaccacaat gctgttgagg ttgaccatta accatagcca tactgcatgt tgggggactg
      241 atcctgccat taatcgctaa tgtggcagtc tctgaagaga atgcgggaga agccaatcca
      301 taggcaagaa aaataattcc tgacagccaa tacttattca ttgaacgtta tccctgtagt
      361 aaaggttatg cctggataga atgagtgcat aacaaactat agctgtacat ccactacaca
      421 gccacgaagg atgataatga agcattgcct gtatgatcaa tcgactttgt agagtttcgg
      481 acgaaggtcc gcagaatatt cgcagtatta aataagtgtt cattaatgcg gcaaaaacta
      541 atacaccgca tcaatgtaac atctctgcgg tgtatgaaga ataaccagaa tggttaatag
      601 gcgttagacc gcgcggaagg aaatctcgcc tggaatgact tcaccctgcc agtagagctg
      661 agctgcaacg cggtcagcca gctggcggta gatagcggta aattcgctct ccggacggct
      721 gataaccgtt ggcgtgcctt tatcgagatc ctcacgtaag gagatgtgta acggcatctg
      781 ccccagcagc tgtgtgtggt atttttcagc cagtttctca gcaccaccgg taccgaaaat
      841 cggttcatgg tgaccacagt tactgcaaat atgcacactc atgttctcga caataccaag
      901 caccggaacc tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat
      961 gtcctgcggg gtggtgacca cgactgcacc agttactgga atgttttgcg ccagcgtcaa
     1021 ttgaatgtca ccggtgcctg gcggcatatc aagcaccaga tagtcgagac ccggccacaa
     1081 ggtttcctgc aacatctgca ttaacgcctt gctcgccatc ggtccacgcc acaccatcgc
     1141 attgtcgtca gtgaccagat aaccaattga attggttgcc aggccatgag acacaatcgg
     1201 tgccatatgg gtaccgtccg gcgaagttgg acgctgattt tctgcgccca gcatggtggg
     1261 aattgatggg ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag
     1321 tgccagcgcc aggtttaccg ccgtggagga tttacccacg ccgcctttac cggaactgac
     1381 agcaatgatg ttcttcacac cattaatacc cggctgattc ttcacgcgtt ttaaggtggc
     1441 gatattgtgc gacagtttcc agtcgatagc tttcgccccg gtgatacgca gcagttcggc
     1501 gctacattgc tcttttaact cttcaaacgc gctatgccag acgaatggca taaccagttc
     1561 cacatgcaac gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg tggtgaggtt
     1621 atgcttcagg gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc
     1681 ttctggtgat ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac
     1741 catcagctga tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt
     1801 ggtactatct aacccctttt cactattaag aagtaatgcc tactatgact caagtcgcga
     1861 agaaaattct ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata
     1921 tgctggagca catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg
     1981 tcaacttcat ctgcgccgac gatgcccacg gtacaccgat catgctgaaa gctcagcagc
     2041 tcggtatcac cccggagcag atgattggcg aaatgagtca ggagcatcag actgatttcg
     2101 caggctttaa catcagctat gacaactatc actcgacgca cagcgaagag aaccgccagt
     2161 tgtcagaact tatctactct cgcctgaaag aaaacggttt tattaaaaac cgcaccatct
     2221 ctcagctgta cgatccggaa aaaggcatgt tcctgccgga ccgttttgtg aaaggcacct
     2281 gcccgaaatg taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct
     2341 acagcccgac tgaattgatc gagccgaaat cggtggtttc tggcgctacg ccggtaatgc
     2401 gtgattctga acacttcttc tttgatctgc cctctttcag cgaaatgttg caggcatgga
     2461 cccgcagcgg tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatccg
     2521 gcctgcaaca gtgggatatc tcccgcgacg cgccatactt tggttttgaa attccgaacg
     2581 cgccgggcaa atatttctac gtctggctgg acgcaccgat tggctacatg ggttctttca
     2641 agaatctgtg cgacaagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagact
     2701 ccaccgccga gctgtaccac ttcatcggta aagatattgt ttacttccac agcctgttct
     2761 ggcctgccat gctggaaggc agcaacttcc gcaagccgac caacctgttt gttcatggct
     2821 atgtgacggt gaacggcgca aagatgtcca agtctcgcgg cacctttatt aaagccagca
     2881 cctggctgaa tcattttgac gctgacagcc tgcgttacta ctacactgcg aaactctctt
     2941 cgcgcattga tgatatcgat ctcaacctgg aggatttcgt tcagcgtgtg aatgccgata
     3001 tcgtgaacaa agtggttaac ctggcctctc gtaatgcggg ctttatcaac aagcgttttg
     3061 acggcgtgct ggcaagcgaa ttggctgacc cgcagttgta caaaaccttc actgatgccg
     3121 ctgaagtgat tggtgaagcg tgggaaagcc gcgaatttgg taaagcagtg cgcgaaatca
     3181 tggcgctggc tgatctggct aaccgctatg tcgacgagca ggctccgtgg gtggtggcaa
     3241 aacaggaagg ccgcgatgcc gacctgcagg caatttgctc aatgggcata aacctgttcc
     3301 gcgtgctgat gacttacctg aagccggtac tgccgaaact gaccgagcgc gcagaagcat
     3361 tcctcaatac agaactgacc tgggatggta tccagcaacc gctgctgggc cacaaagtga
     3421 atccgttcaa agcgctgtat aaccgcatcg atatgaagca ggttgaagca ctggtggaag
     3481 cgtctaaaga agaagtgaaa gcagctgccg cgccagttac tggcccactg gcagatgatc
     3541 cgattcagga aaccatcacc tttgacgact tcgcaaaagt tgacctgcgc gtggcgctga
     3601 tcgaaaacgc agagtttgtt gaaggttctg acaaactgct gcgcctgaca ctggatctcg
     3661 gcggtgaaaa acgcaatgtc ttctccggta ttcgttctgc ctacccggat ccgcaggcac
     3721 tgatcgggcg tcacaccatt atggtggcca acctggcacc gcgtaaaatg cgctttggta
     3781 tctccgaagg catggtaatg gctgctggtc ctggcgggaa agatatcttc ctgttaagcc
     3841 cggatgctgg tgctaaacca ggccatcagg tgaaataatc cccctttaag gcgctgcatc
     3901 gacagcgcct tttctttata aattcctaaa gttgttttct tgcgattttg tctctctcta
     3961 acccgcataa atactggtag catctgcatt caactggata aaattacagg gatgcagaat
     4021 gagacacttt atctatcagg acgaaaaatc acataaattc tgggcagttg agcaacaggg
     4081 aaacgagttg catatcagtt ggggaaaagt tggcaccaaa gggcaaagcc agataaaaag
     4141 tttttcagat gctgcggcag tggcaaaagc ggagcttaag ctgattgcgg agaaggtgaa
     4201 gaaggggtat gtggagcaag cgaaggataa ttctttgcaa ccttcccaaa cggtaacggg
     4261 ctctctcaag gtagcggact tatccaccat tattcaggaa caaccctctt tcgtagcaga
     4321 aacccgtgcg cctgacaaaa atacagatgc tgttttaccg tggctggcga aagatattgc
     4381 tgtcgttttt ccgcccgaag ttgtacacac cacgttaagt catcgccgct ttcccggagt
     4441 tcctgttcag caagcagaca aattgaccca attacgtcgc ttagcctgta gtgtgtcgca
     4501 acgggataat aaaacagcca catttgactt cagcgcctgt tctttagaat ggcaaaacac
     4561 cgtcgcccag gcgatcagtc agatcgacgg cctgaaaaca acacagctac catcaccagt
     4621 aatggctgta ctcacggcac ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt
     4681 tatggatcag atcgtccagg aaggcggtct ggaatatgct actgatgtaa taatacacct
     4741 tcaacagatt gatattaaat gggattatgc gaataatgtc attattattc tgccgtctgg
     4801 cattgcacct gactacttgg agcaatattc cagatttgaa ttacgcctac gtaaacattt
     4861 atcactggcg gaagagtctc tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc
     4921 acatattcca gaatggcggc aaccattaat tgctttgtta ttacccgaaa aaccagaaat
     4981 tgcacatgaa attgcccagc gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt
     5041 aaaaatagtg gcgactgatg agcacattct tgcctcatta gaaaaatatc acgagccata
     5101 tgccattttt gatgattact attgtggtgc gatatggtca gccaccgtat tacaggagca
     5161 aggtgttgca gccctgcccc gatttgctcc ctatgccgca agtgactact gcgccgatgt
     5221 gttgcgtcat atcaatcatc cgttcgcatt aacactgctt atacgtgtag ccgggcatac
     5281 taaacgctgt cacgatcgga tgacgaaagc ctgcgctgcg ttcccacatg cagcaatggc
     5341 ggcactgacg gaacttcttg ggcaaaaaga agagaacagt tggcgcatta tgctaatgac
     5401 aatgcttatc tcacaaccag cactggcaga acaggtcatt ccctggctct cgacacccgc
     5461 agttgccgta ctgaaatcat gccagcaaca actgacacag ccctcaaacc atgccagcgc
     5521 cgatctactg ccagccgtag tagtctcccc tccctggctt tcgaaaaaga aaaaatcgcc
     5581 gattccggtg ctggatttag cgccattagg cattgagcca atctgttatc tgacagaaga
     5641 aatcagtaat caacttttgg cgaaatatat ctggtattca aaacacatca cggttagcca
     5701 tgaagaaagt actaccaacc tgttggcaag gatgggtttt caacgacgga tcgctggtac
     5761 atatattaaa gctcccgaag cggtagttga ggcatggcta aatgaagatt attcaacctt
     5821 actaagtgaa tttaaggtgt ttcattcacc taccgggcat tattggcagt tggggatttt
     5881 gacaacattg ccgctggaga aagcagtaaa agcatggaat gcccttaccc tatctccaca
     5941 taccgatacc gaatacgcca tgttacattt tggactcaaa gggttacctg ggttagtaaa
     6001 ctcacttgca cgctatccac aagaagcctt gcccatcacg aattacttcg cagcgagtga
     6061 gctggctcct gccgtcgccc gtgccttcaa caaactgaaa acgctacgcg aaaacgcccg
     6121 tagctggctg ttgaaatacc cggaacatgc ccttaccggc ctgctgcctg cggcgctcgg
     6181 caaagccggt gaagcacagg ataacgcccg cgctgccttg cgtatgctta ccgaaaacgg
     6241 tcatcagcca ttactgcaag aaatcgcccg acgttataac cagccggaag taaccgatgc
     6301 ggtgaacgct ctgcttgcgc tcgatccctt agataatcac ccgacaaaaa tccccactct
     6361 tccggccttt tatcagccat cgctctggac gcgcccggta ttaaaagcaa atgcccaatc
     6421 actgccagat agcgccctcc tccacctcgg tgaaatgctc cgcttccctc aggaagaggc
     6481 tctgtatccg ggattattgc aggtgaaaga cgtctgttcc gccgactcac tggcgggatt
     6541 tgcctgggat ctgtttaccg cctggcagac cgctggcgcg ccgtcgaaag agagttgggc
     6601 gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc cgcaaactga cgccattaat
     6661 acgcgcctgg cctggtgaat cccagcataa acgcgccacc gttgggttgg atattctcgc
     6721 tgctatcggt agtgatatcg cccttatgca gcttaacggc atcgcccaga aactgaaatt
     6781 caaagcatta caggagcggg caaaagaaaa aattgccgac attgccgaga gccgcgaact
     6841 cacggtggcg gagcttgaag atcggttagc accggatctc ggtctggatg ataacggttc
     6901 gctgctgctg gattttggcc cacggcagtt caccgtcagc tttgatgaaa ccttaaaacc
     6961 gtttgtgcgt gatgtttccg gcagccgcct gaaagacctg cccaaaccga acaaaagcga
     7021 tgatgaaacg cgggcgaacg atgcggttaa ccgctacaaa ttgctgaaaa aagatgcgcg
     7081 taccatcgcc gcccagcagg tagcaaggct ggaatccgcc atgtgcctgc gccgccgctg
     7141 gtcgctggaa aacttccagc tcttcctggt tgagcatccg ctggttcgtc acttaacccg
     7201 ccgtctgatt tggggcgttt atagcgccga aaaccagcta ctggcttgct ttcgcgtagc
     7261 agaagataac agctacagca ccgctgacga tgatcttttc accctgccgg aaggcgatat
     7321 ctctatcggc actcctcacg ttctggaaat atcaccaacg gatgctgccg cctttggtca
     7381 gctttttgcc gactacgaac tgctaccacc gttccgccag ctcgaccgta acagctacgc
     7441 cctgacagaa gccgagcgca atgccagtga actgacccgc tgggcaggca gaaaatgccc
     7501 gagcggtcgg gtcatgggac tggcgaataa aggttgggtg cgtggtgaac cgcaggatgg
     7561 tggctggatc ggctggatga tcaaacctct gggccgctgg tcgttaatca tggaaatcga
     7621 tgaaggcttt gcagttggta tgtcgccagc cgaacttagc gccgagcaac tcttaagcaa
     7681 gctgtggcta tgggaaggca aagcagaaag ctatggctgg gggagtaatt caacacagga
     7741 agcgcagttc tccgtactcg atgccatcac cgccagcgag ctgattaacg atattgaagc
     7801 cctgtttgaa taaggaaaga gcatggacaa ggaattaccg tggctggcgg ataacgccca
     7861 actggaactg aaatataaaa aaggcaaaac accactcagc catcgcaatt ggccgggcga
     7921 accagtgcct gttatcactg aaagcatcat tcagacactg ggtgatgaat tgctacaaaa
     7981 agctgagaag aaaaaaaaca ttgtctggcg ttatgagaat ttttcactgg agtggcagtc
     8041 cgccattacg caggccatca acttgatcgg cgaacacaaa ccctcaatcc cggcccagac
     8101 aatggcggcg ctagtctgta tcgcgcaaaa tgacagccaa cagttgctcg acgaaatcgt
     8161 ccaacaagag gggctggaat atgcgactga ggtggtgatt gcacgccagt ttattacacg
     8221 atgttatgag agtgatcctc tggtagtcac attgcagtat cagaacgagg actatggcta
     8281 tggttatcgc tcagaaacct ataacgaatt cgatctccga ctgcgtaagc atctctctct
     8341 ggcagaggaa agctgctggc agcgttgcgc cgacaaactc attgccacac tgccaggaat
     8401 aaccaaagtt cgccgccctt ttattgcgct gatcctcccc gaaaaaccag aaatcgccaa
     8461 tgagttggta agccttgaat gcccgcggac tcattttcat tctaaggagt ggttaaaagt
     8521 tgttgctaat gaccccacag cggtgagaaa actcgaacgc tactggagcc aggatatatt
     8581 tagcgatcga gaagccagct acatgtcgca tgaaaaccac ttcggctacg cggcctgcgc
     8641 cgcccttttg cgcgaacaag gactggcagc cgttccgcgc ctcgcgatat atgcccataa
     8701 agaagattgc ggcagtctgc tggtacaaat taaccatccg caagtcatcc gcaccttgct
     8761 gttagttgct gataaaaaca aacccagcct gcaacgtgtg gctaaataca gtaaaaactt
     8821 cccccatgcg acactcgccg cactggcaga actgctggcg ttaaaagaac caccagcccg
     8881 cccaggttat ccaatcatcg aagacaaaaa gctgcctgca cagcaaaaag cacgcgatga
     8941 atactggcgt acgctgttac aaacgctgat agcatcgcag ccacaactag cagaaagggt
     9001 gatgccgtgg ttaagtactc aagccagggc agtgctgaat agttatttat cggcaccgcc
     9061 aaaaccggtt attgatagca ccgataacag cagtctgcct gaaatgctcg tttcaccacc
     9121 gtggcgtagt aagaaaaaaa tgacagctcc acgtcttgat ttggcaccgc tcgaattaac
     9181 tccgcaaatt tactggcaac caggcgaaca agagaggctt gccgccactg agtctgcccg
     9241 ttatttcagc acggaatctc ttgcggaacg catggaacaa aaaagtggac gagttgtatt
     9301 acaggaactg ggttttgggg atgatgtatg gctgtttctg aattatatac tccccggaaa
     9361 actggatgct gcacgcaatt cactcattgt tcagtggcat tactaccagg ggcgggttga
     9421 agagatcctg aatggctgga actccccgca agcacaatta gcagaacagg cgctccgcag
     9481 cggtcacata gaagcgttaa ttaacatatg ggaaaatgac aacttctcac gttatcgtcc
     9541 ggaaaagagt gtctggaacc tgtatttatt ggcacagttg ccgcgtgaga tggctttgac
     9601 cttctggctg cgtatcattg agaaaaagca tctgttcgcg ggtgaggact attttctcag
     9661 tatcctcgga ttggatgcgc taccaggtct gctgttggct ttttcacatc gtccaaaaga
     9721 aacatttccg ttaattttaa attttggcgc aacagaactg gcgctgcctg ttgcccgcgt
     9781 ctggcgacgt ttcgcggcgc agcgtgatct ggctcgccag tggattttac aatggccgga
     9841 acatacggct agtgcactta tccctcttgt ctttaccaaa cccagcgata atagcgaagc
     9901 cgcattactt gccctgcgtt tactgtacga acagggacat ggcgaattgc tacaaaccgt
     9961 ggcaaaccgc tggcagcgta cagatgtatg gtctgccctg gagcagttgc ttaaacaggg
    10021 tccaatggac atttacccgg cacgcattcc aaaagcccct gatttctggc atccggcaat
    10081 gtggtccagg cctcgactta tcactaataa tcagcctgtt accggtgacg ctctggaaat
    10141 tatcggcgaa atgctgcgct ttacccaggg gggacgtttt tatagcgggc tggaacaact
    10201 gaaaacgttc tgccagccac aaacgctggc agcttttgcc tgggatctct tcactgcgtg
    10261 gcaacaagct ggtgcccccg caaaagacaa ctgggcattt ctggcgttaa gtctctttgg
    10321 tgacgaaagc acggcacggg atctgacgac acagatcctc gcctggccac aagaaggcaa
    10381 atctgcccgt gctgtcagcg gcctgaacat ccttaccctg atgaataatg atatggcgct
    10441 gatacagctg catcatatat cgcaacgggc taaatcccgc cccttacgtg ataacgcggc
    10501 ggaatttctt caggtggtcg cagaaaatcg cgggctaagc caggaagagc tagcggacag
    10561 attagtccca accctgggcc ttgatgatcc gcaggcgttg agttttgatt ttggtccccg
    10621 gcagtttacc gttcgcttcg atgaaaacct caacccggtt atctttgatc agcaaaacgt
    10681 tcgccagaaa agcgttcccc ggttgcgcgc cgatgacgat caactgaaag cgcccgaggc
    10741 actggcccga ctaaaagggc taaaaaaaga tgctactcag gtgagcaaaa acctgctccc
    10801 gcgtcttgaa gctgccctac gtaccacccg acgctggtcg ctggcagatt ttcatactct
    10861 gtttgttaat catcccttta cccgcctggt tacccagcga ttaatatggg gcgtgtatcc
    10921 ggcaaatgaa ccgcgtcgtt tactcaacgc ctttcgtgtg gccgcagagg gggagttctg
    10981 caatgcgcag gatgagccaa ttgacctgcc tgcggatgct ctgattggca ttgcccaccc
    11041 gttagaaatg acagcagaaa tgcgcagtga atttgcccag ctttttgccg attacgaaat
    11101 tataccgcca cttcgccagt tgacacggcg cacggtgctg ctcacgcctg acgagtcagc
    11161 cagtaatagt ttgaatcgct gggaaggtaa atctgctacc gttgggcaac ttatgggaat
    11221 gcgatacaaa ggctgggagt caggctatga ggacgcattt gtctatgacc tgggtgagta
    11281 ccggctggtc cttaagtttt cacccggttt taaccactac aatgttgata gcaaagcgct
    11341 aatgagcttc cgttctcttc gagtgtacag tgacaataaa tccgtcactt ttgccgaact
    11401 tgatgtgttt gatttgagtg aggcgttaag cgcacctgac gtcattttcc attaaaacac
    11461 agcgggcagt gatgcaactg cccgttatca acacgactta ccagcgggga aagcgatgat
    11521 cgtgcaaaaa gagctggttg ctatttacga ttatgaggtc cctgtacctg aagatccgtt
    11581 ttccttcaga cttgagatcc ataaatgctc tgaattattt acaggttccg tctatcgact
    11641 ggagcgattc cggctacgtc caacatttca tcaacgtgat cgagaagatg ctgacccgct
    11701 aataaatgat gcgttgattt atataagaga tgagtgtatt gatgagcgga aattacgagg
    11761 tgaatcacct gaaactgtaa tagcaatttt taatcgtgaa ctacagaata tattcaacca
    11821 actaatagaa tattagttta ttaaaggggg aagcggtttt cccccttagt tgcgtttcaa
    11881 tttgcccatt acttcaacaa atcaatcaac cgtaacagag acactaatag ctccagtacg
    11941 gcaatgagca cggttaactt ttgtgtcagc gtgtccatcc gctctcctta ttaaggagcg
    12001 caacaccagc accttaccct tacactgcct gtcgatactg gtttacgttt gttgagtcgc
    12061 catgttgcac tccggatcag agcactacct gcggcaccac ccgtccggta gccaggactt
    12121 gaaatgggtt cgatccggcg caacttccca acagtcagga ttataaatct cattatctta
    12181 attgcaacac tgtccagagg tggttataat tcgttcgttg agatgccatg ttgcactatc
    12241 agtaacttaa catcaaagta gtaaataaaa gaacctgcct caccagcagg tttttttatt
    12301 ttctgcgatc tactttccag atattttttg ccaatagccc accataaata ttcacatccc
    12361 ggtcaaatgg tgcgttgaat ataaaatcga tgaatttata aataaaaatg agttatatga
    12421 tgaattaata aaaacagtaa tcattttatt aaagggggaa gcggttttcc cccttagatg
    12481 cgtttcaatt tgcccgttac ttcaacaaat caatcaaccg taacagagcc actaatagct
    12541 ccagtacagc aatgagcacg gttaactttt gtgtcagcgt gtccatccgc tctccttatt
    12601 aaggagcgca acaccagcac cttaccctta cactgcctgt cgatactggt ttacgtatgt
    12661 tggaccacga tgttgcactc cggatcaggg caccacctgc agcaccaccc gtccggtagc
    12721 caggacttga aatagattcg atccggcgca acgtcccaac ggtctggatt ataaatctca
    12781 ttatcttaat tgcaacgggg tccagccgtg gttataattc gttcgttgga acacgatgtt
    12841 gcacaggctg tggtgttagg cctgaaagta gtaaataaaa gaacctgcct caccagcagg
    12901 tttttttatt tactgtgatc tgctttccag atatttttcg ctcaaacaac taatgcgcca
    12961 aacatttatt gcgcgtaaaa tatcgtttat ttcattaata catttcaggg atgaatatat
    13021 gtcaccgcag aataatcatc tgcagcgtcc gcctgctgct gtgttatacg ccgatgaact
    13081 ggcaaaatta aaacaaaatg ataacgcacc ttgcccgccc ggttggcagt taagtttgcc
    13141 tgcggcccgt gcttttatcc ttggcgatag cgcgcaaaat attagccgta aagtggtgat
    13201 tagtccctcc gctgtcgaac gcatgttagt cactcttgcc accgggcgcg gtttgatgtt
    13261 ggtgggggaa cctggcaccg caaaatctct cctgtctgaa ttactggcaa ccgctattag
    13321 cggcgatgcc gggttaacca tccagggcgg cgcatctact accgaagatc aaatcaagta
    13381 cggctggaac tacaccctgc ttatcaacca tggtccgtca acggaagcac tggtccctgc
    13441 cccgctttac cagggaatgc gcgacggcaa aatcgtccgt tttgaagaga taacgcgtac
    13501 accgctggaa gtacaagact gtctgttagg tatgctttct gacagggtga tgacggtgcc
    13561 ggaactcacc ggtgaagcca gtcagctcta tgcgcgggaa ggctttaata ttattgccac
    13621 tgccaatacc cgcgatcgcg gcgtcaatga gatgagtgcc gcgctaaagc gtcgcttcga
    13681 ttttgaaacc gtctttccga ttatggattt cgcccaggag ctggaactgg tcgccagtgc
    13741 ttcggcgcgt ttgctggccc atagcggtat tccgcataaa gtgcctgacg cagtactgga
    13801 gttactggta cgcaccttcc gcgatctgcg tgccaacggc gaaaagaaaa cgtcaatgga
    13861 tacactgaca gcgattatgt ccaccgctga agccgtgaac gtggcccacg ccgtaggcgt
    13921 ccgcgcctgg tttttagcga atcgcgcggg ggagcctgcc gacctggtgg actgtattgc
    13981 cggaaccatc gtcaaagata acgaagaaga tcgcgctcgt ctgcgccgtt acttcgaaca
    14041 gcgtgtcgcc acacataaag aagctcactg gcaggcttat tatcaagccc gccaccgcct
    14101 gccgtgagga aagaggcatg agcgagccgt taattgtcgg catccggcat catagtccgg
    14161 cctgcgcccg gctggtgaaa tcgttaatcg aaagccagcg gccacgatac gtgttgattg
    14221 aaggcccggc tgattttaat gaccgggtag acgaactttt ttttcccacc agcttccggt
    14281 agctatttac agttattgcc agtatcagga cggtgcagcc cccgggcgtg gtgcctggac
    14341 gccatttgct gaattttcgc cggagtggca gacgctacaa gccgcacgtc gtattcaggc
    14401 acaaacttac ttcatcgatt tgccttgctg ggcgcagagt gaagaagagg acgattcgcc
    14461 tgatacgcaa gatgaaagcc agcaaactgc attaccctct gcgctggcgc actattttgc
    14521 ccaactgcgg ggcgatttcc ccggcgatgc actcaatcgt cagcgcgaag cctttatggc
    14581 tcgctggatt gcatgggcgg tgcagcaaaa taatggcgac gtgttagtcg tctgcggtgg
    14641 ctggcacgct ccggcactgg caaaaatgtg gcgcgaatgc ccgcaggaca ttaacacgcc
    14701 ggaattgccc tcgctggcag atgccattac aggttgttat ctcacgccct acagtgaaaa
    14761 gcgccttgat gtgctggcag gatacctttc cggtatgcct gccccggtct ggcaaaactg
    14821 gtgctggcag tggggcttac agcaggccgg tgaacaactg ctaaaaacta tccttacccg
    14881 tttgcgccag cacaagttgc ctgcttcgac cgcggatatg gctgccgctc atctgcatgc
    14941 gatggcgctg gcacagttgc gcggtcatac actaccgtta cgcactgact ggctggatgc
    15001 catagcagga tcgctgatta aagaagccct gaatgcgccg ttgccgtgga gctatcgcgg
    15061 cgttattcat cccgacaccg atccgattct gctaacgttg atagacacat tagcgggtga
    15121 cggattcggt aaacttgccc cttctacgcc acaacctcct ctgccaaaag atgtcacctg
    15181 cgaactggaa cgtaccgcaa tctcccttcc ggcggagctt accttaaatc gctttaaccc
    15241 caatggacta gcgcaaagtc aggtgttaca tcggctggca atactggaga tcccagggat
    15301 tgtgcgccag cagggaagca cactgacact tgcaggcaac ggtgaagaac actggaaatt
    15361 aacccgcccg cttagccagc atgcggcatt gattgaggcc gcctgctttg gtgccacact
    15421 ccaggaagcc gcacgccata aattagaagc cgatatgctg gacgcgggtg gaatcggcag
    15481 tatcaccaca tgtcttagcc aggcggcgtt agcgggtctg gcgtccttca gtcaacaatt
    15541 actggagcaa ctcacattat taatcgccca ggaaaatcaa tttgccgaaa tgggccaggc
    15601 gctggaagtg ctatatgcct tatggcggct ggatgaaatt agcggtatgc aaggcgcgca
    15661 gatattacaa acgacgttat gcgcggctat cgatcgcacg ctgtggctgt gtgaatctaa
    15721 cggcagaccg gatgaaaagg agtttcacgc tcacctgcat agctggcaag cgctttgcca
    15781 tattctgcgc gatctacata gcggcgttaa tttatccggc gtttcgcttt ctgcggcggt
    15841 agccttactg gagcgacgca gtcaggcaat tcatgccccg gcgctggatc gcggcgcggc
    15901 tcatggcgca ctgatgcgtc tggaacatcc taacgccagt gccgaagcgg cgctgacgat
    15961 gctggcgcag ttatccccgg cacaatccgg cgaagcgctg cacggtttgc tggcgctggc
    16021 ccgccatcaa ctggcctgtc agccgacatt tatcgccggt ttcagcagtc atttaaatca
    16081 actaagtgat gccgatttta tcaatgccct gcccgattta cgcgcggcga tggcctggct
    16141 accaccacga gaacgcggga cgctggcgca tcaggtgctt gagcattatc aactggcgca
    16201 acttcccgtt tcggcgctgc aaatgccgtt gcattgtcca ccacaggcca ttgcacatca
    16261 tcaacaactc gaacagcagg cactggcatc gctgcaaaac tggggagttt tccatgtctg
    16321 aactgaacga tcttctgacc acccgtgagc tacaacgctg gcgattaatt cttggcgaag
    16381 cggcagaaac gacgctttgt gggctggatg acaacgcccg gcagatagac cacgcgctgg
    16441 agtggctgta tgggcgcgat cctgaacggc tccagcgtgg tgaacgctct ggtggattag
    16501 gtggctcaaa tctcaccacc cctgagtgga tcaacagtat tcacactctg tttccgcaac
    16561 aggtgattga gcgactggaa agcgatgccg tactgcgcta cggcattgaa gatgtggtga
    16621 cgaatctcga cgtgctggaa cgtatgcagc cttcggaaag cctgctacgc gccgttttgc
    16681 acaccaaaca tctgatgaat cccgaagtac tggctgccgc ccgccagata gtgcgccagg
    16741 ttgttgaaga aattatggcg cgactggcaa aggaagttcg tcaggctttt tctggtgtcc
    16801 gcgatcgccg tcgccgctca ttaattccac tggcgcgaaa ctttgatttc aaaagtactc
    16861 tgcgcgccaa cctgcaacac tggcacccgc aacacggcaa gttgtatatc gaatcccccc
    16921 gctttaacag ccgcattaaa cgccaaagcg aacaatggca actggtctta ctggttgatc
    16981 aaagcggatc gatggtggac tcggtgatcc actctgcggt gatggcggcc tgtttgtggc
    17041 agttacccgg cattcgtacc catctggtgg cgtttgacac aagcgtggtt gatctcacgg
    17101 cagacgttgc cgatccggta gagttattaa tgaaagtaca gttgggcggc gggaccaata
    17161 tcgccagtgc cgtggagtat ggtcggcaac ttattgaaca accagcaaaa agcgtcatta
    17221 tcctcgtgag cgatttttac gaagggggtt catcatcatt gctgacgcat caggtaaaaa
    17281 agtgtgtcca gagcggcatc aaagtgctgg gactggcagc gctcgatagc accgcaacgc
    17341 cttgctatga ccgcgatacg gcccaggcgc tggttaatgt cggcgcacaa atagccgcca
    17401 tgacgccggg cgagctggca tcatggcttg cggagaatct tcagtcatga attcactacg
    17461 tccggaatta ttagaactga caccgcaggc actgacggcg ttaagcaatg ccggttttgt
    17521 taagcgcagt cttaaggaac tggaaaatgg caacgtcccg gaaatcagcc atgagaacgg
    17581 cgctttaatc gccaccttca gtgacggtgt ccgtacccag ctggcgaaca gccaggcact
    17641 gaaagaggct cagtgcagct gcggggccag cggcatgtgc cgtcatcgcg tgatgctggt
    17701 gttaagttat caacgacttt gtgccaccgt ccagcccacg gaaaaagaag aagagtggga
    17761 tccggcaatc tggctggaag aactggctac cctgcccgat gccacccgca aacgcgcgca
    17821 ggcgctggtc gctaaaggca tcaccattga gttgttctgt gcgccgggcg aaattccctc
    17881 tgcccgctta ccgatgagcg atgtgcgttt ttattcccgt agcagtattc gtttcgcccg
    17941 ttgtgattgt attgaaggca cactttgcga acatgtcgta ctggctgtgc aggccttcgt
    18001 cgaggccaaa gcgcagcaag cagaatttaa ccatttaatc tggcagatgc gcagcgaaca
    18061 cgtcacatca tctgacgatc cgtttgccag cgaagaaggc caaacgtgtc gtcaatatgt
    18121 tcagcaatta agccaggcat tatggcttgg cggcatcagc cagccgctta ttcactacga
    18181 ggcagcattc aaccgcacat tgcaggcggc agaggcctgc aactggcgct gggtgagtga
    18241 atcactacga cagctacggg caagcgttga tgccttccac actcgcgcca gccactatca
    18301 tgccggagaa tgcttacgtc agcttgcggc attaaacagt cgattaaatt gcgcacaaga
    18361 gatggcccgg cgcgacagtg ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg
    18421 ctctggcatt gccggagaag caaagcttga tcatctgcgg ctggtgtctt taggtatgcg
    18481 ttgctggcag gatattgagc attatggttt acgcatctgg tttaccgatc ccgacaccgg
    18541 cagtattttg cacctttcgc gcagttggcc gcgaagtgaa caggaaaact caccggcagc
    18601 tacgcgtcgg ctgtttagtt ttcaggctgg cgcactggcg ggtgggcaaa ttgtttcaca
    18661 agcagcaaaa cgcagtgccg atggcgagct gctgttagct acccgcaacc gcttaagcag
    18721 cgttgtgccg ctgtcgcctg atgcctggca aatgttgagc gcgccgttac gccagccggg
    18781 cattgtggct ttgcgggaat atttacgcca gcgtcccccc gcctgcatac ggcctcttaa
    18841 tcaggtcgat aacttattta ttctgccggt cgctgagtgt atttcgctcg gttgggatag
    18901 cagccgccag acgctggatg cgcaggtaat cagcggcgaa ggtgaagata atctcctgac
    18961 gttatccctt ccggcacctg cctgttctcc ttttgccgtt gaacgcatgg cggcgctttt
    19021 gcaacaaaca gacgaccccg tgagtctggt ttctggcttt gtcagttttg ttgatgggca
    19081 attgacactg gaaccacggg tgatgatgac aaaaacccgc gcctgggcgc tggacgcaga
    19141 aactgcgcct gtggcaccgc taccttctgc cagcgttttg cctgtgccgt ctaccgctca
    19201 tcagttgctg atgcgctgcc aggcgttact tattcaactg ctccataacg gctggcgcta
    19261 tcaggaacag agcgctatta gtcaggcaga gttgctggcg aatgacctct ccgcggtcgg
    19321 tttttatcgg ctggcacatg tgttgggaca atttcgtaat acagaaagcg aggcacgggt
    19381 agaagcaatg aataacggtg ttttactttg cgaacaatta ttccccatgc ttcagcaaca
    19441 aggatgaaat agtgcttttt actaagagtt ctactccagt tccggactgc tcacgtcacg
    19501 gtattaggca tattctatat agcccctggt gagagtcacc agtttcttga ttaaataaaa
    19561 tggagtttta catgaaggct ttcaataagc tgttttccct cgttgttgca tctgttctgg
    19621 ttttctctct tgctggctgc ggtgacaaag aagaatcgaa gaaattcagc gccaatctga
    19681 acggcactga aattgccatt acctatgtct acaaaggtga caaggtgctt aagcaatctt
    19741 ctgaaaccaa aattcaattt gcttctattg gtgcaaccac caaagaagac gctgccaaga
    19801 cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg tgttgaagaa aaattaacct
    19861 atacagatac ctacgcgcag gaaaacgtga ctatcgatat ggaaaaagtg gattttaaag
    19921 ccctgcaggg tatttcagga atcaacgttt ctgctgaaga tgccaaaaaa ggtatcacta
    19981 tggcgcaaat ggaactggtg atgaaagccg ctggttttaa agaagtgaaa taatctgtcg
    20041 gcggccatgt ttcgcatggc cgccataccc gtcttagctt ttcttcacat gctggcgcgc
    20101 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
    20161 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
    20221 gaggatagcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac
    20281 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
    20341 tttttcataa atcaggccat tgaggaatga cgacaaaaca atgtccggac aacgctgaaa
    20401 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
    20461 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
    20521 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
    20581 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
    20641 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
    20701 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
    20761 tttccagggt acgtaatgtc aattcggtaa agccctcttt cccttcgtgg ctggtaacgt
    20821 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
    20881 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
    20941 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
    21001 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt
    21061 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc ccaaccattt
    21121 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
    21181 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
    21241 gctcctgcaa aaatacacgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
    21301 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
    21361 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
    21421 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
    21481 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
    21541 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
    21601 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
    21661 gaagcgcgcc ttttcaattt gcagataagc gttcacatgt tcaatttcgt cggcgagagt
    21721 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
    21781 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
    21841 caaaaaatga ggattcacct gggcgtgaag cagtttgatt tctgactggg tgagcatcgc
    21901 tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa agcagttgcg caatcccctc
    21961 gcccagcgtg cggtttattg aactgaataa acggtttttg gcttcataca atttgatggt
    22021 gcccatcacg cgctgatttt cgccacgcaa cggaattacc agcgtcgacc ccagtttgca
    22081 ttgcggatgc aaagagcaac gataaggtac ttcgttgcca tcagcgtaga ccacttcacc
    22141 ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt ttgccgggta aatggtggtc
    22201 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
    22261 atccagctcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
    22321 tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagcagaagt
    22381 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
    22441 ggtattggtg accatcattg gtgcagcaat attactcacc agacgcaccg catcttcata
    22501 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
    22561 accggcggta atggggttaa agactttatc agtgcgcccg cgacggatca ggatgctgtg
    22621 taccaggccg ccgagcaatc cttcaacgat ggttgagatc atgcaactta gcgcggtcat
    22681 gccccccatc gaatatcgat gtaagccgcc ggtcagacca accagcccac cgacgaccgg
    22741 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
    22801 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
    22861 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
    22921 cattaaccac gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
    22981 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
    23041 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
    23101 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
    23161 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
    23221 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt
    23281 gacgcaacat ttttaaaaat caacgaagat cgtccaggag cgccgatttt caagggttgg
    23341 acagataatt cgttttcgat gtcgacgaac ttcgacggat ttcgcctata aaaagtgtcc
    23401 catacatgcc ccattgatgc cccatagaca atatcagcaa ttgacactgg ttatctatac
    23461 aactaaaaat aacacctgca cataaccaca gttacaggag ggtgttatgc gtgttgagag
    23521 ctgtatcgct aaagacaaaa tcagcaaaat gaggtgtact ggcaatagcg gacactacca
    23581 tttgttcttt ttttaagcag ccatctgatg atattaatag cagatatgtt tgcagcaaag
    23641 cccgaattaa gtcaaagatg tatttctgga ctcctcacaa ctgggccaaa tgatgaaaac
    23701 gtaaataata aaccgtcaag tgatatgccg aacagaatca aaccagttgt aagcgattca
    23761 caaatcggta tatatacagc tgacagtaat actctgaagg atggattgtg tagccaagcc
    23821 cacataggaa ctcaacctta tcggccaggt gctgactcgc cgataactgt gagatcattc
    23881 ctacaaatca cactttccaa caataattac aaatcattag agcatctgca aacacgagca
    23941 gagaattatc tgaggtatcg caaagcagaa gagaatattc tgcgttcgac cgttgagggg
    24001 ctaaccaatc cagaatctcc tgtattcaaa caaacagcat ggatggggca tctggagcgt
    24061 ggcctttgga aaacggaaac acgctgggat ggaaacgacc gcgagcagct aggcaaagag
    24121 gctcttggct cagaggagcc aaaacctggt tcaccatttt acggtagtcg tggactaaaa
    24181 ttatccgaca gtgcgcattc tgccttctca atgatgctgt gcggttctga aggtccattc
    24241 acaaaagaac aagcactctc tggtttcgag cttgctcaaa ctggccaagt gctggctgga
    24301 aggctaaaga ttcaggagcg agttaaattc cgcgcagaca accgcattga tgctcagcgt
    24361 aacggtacac actcaacccg cacaccaaca ggcatggatc tttctcaaga tatcggcaca
    24421 attatgcgtg ataaagctgg gctaccggtc atgagcggaa catccggttc ctcctctgac
    24481 gccactcttg caacacgtta cgctgccgag cattttggta agacatgggc cgctccaggc
    24541 ttaagccaag cagaaggatg caaagctatc tcggatctgt cacatcacta ttttcgtgca
    24601 gaaggctcca gtccaccaca atcaatggca acgggaataa ataaggttcg ttatgatgca
    24661 ggaatggaag agaaatatgt aaatacactg gatatattta cacatagcta cccagaaatt
    24721 tatgctgggg ttgcattgac gattgctggg gctggcggta atgatgagca ggcaatgtat
    24781 aatgttacgc aagaagccgc tcgtattctg cacgaagctg aaactaaaga ttgaacatgt
    24841 tctagtgaag aggcctaccc cagaagaata tattccacat tcaaggcatt attgatgaat
    24901 tttataatca atgcgaaaaa actggcattg attgtataca gattctcctg aaggagaatc
    24961 tgttctgtcc cttctgccaa gcagtgttac gcaacgatca atctttcgcg ttcctattaa
    25021 tcaatgagtt agagttgatt agtgacctga gacagagtat tagcgcaagg tgattttttg
    25081 tc
//
LOCUS       DAZFYC010000071        24730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFYC010000071 DAZFYC010000000
VERSION     DAZFYC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.071"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..346)
                     /gene="yaaX"
                     /locus_tag="ACHKW5_003125"
     CDS             complement(50..346)
                     /gene="yaaX"
                     /locus_tag="ACHKW5_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO7512475.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(560..1846)
                     /gene="thrC"
                     /locus_tag="ACHKW5_003126"
     CDS             complement(560..1846)
                     /gene="thrC"
                     /locus_tag="ACHKW5_003126"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO7512476.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVESILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1847..2779)
                     /gene="thrB"
                     /locus_tag="ACHKW5_003127"
     CDS             complement(1847..2779)
                     /gene="thrB"
                     /locus_tag="ACHKW5_003127"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO7512477.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2781..5243)
                     /gene="thrA"
                     /locus_tag="ACHKW5_003128"
     CDS             complement(2781..5243)
                     /gene="thrA"
                     /locus_tag="ACHKW5_003128"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO7512478.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5269..5388)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5324..5389)
                     /gene="thrL"
                     /locus_tag="ACHKW5_003129"
     CDS             complement(5324..5389)
                     /gene="thrL"
                     /locus_tag="ACHKW5_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO7512479.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5603..6289)
                     /gene="yjtD"
                     /locus_tag="ACHKW5_003130"
     CDS             complement(5603..6289)
                     /gene="yjtD"
                     /locus_tag="ACHKW5_003130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7512480.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6689..6829)
                     /gene="yjjY"
                     /locus_tag="ACHKW5_003131"
     CDS             complement(6689..6829)
                     /gene="yjjY"
                     /locus_tag="ACHKW5_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO7512481.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6925..7641
                     /gene="arcA"
                     /locus_tag="ACHKW5_003132"
     CDS             6925..7641
                     /gene="arcA"
                     /locus_tag="ACHKW5_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO7512482.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7701..9053)
                     /gene="creD"
                     /locus_tag="ACHKW5_003133"
     CDS             complement(7701..9053)
                     /gene="creD"
                     /locus_tag="ACHKW5_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO7512483.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9111..10535)
                     /gene="creC"
                     /locus_tag="ACHKW5_003134"
     CDS             complement(9111..10535)
                     /gene="creC"
                     /locus_tag="ACHKW5_003134"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO7512484.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10535..11224)
                     /gene="creB"
                     /locus_tag="ACHKW5_003135"
     CDS             complement(10535..11224)
                     /gene="creB"
                     /locus_tag="ACHKW5_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO7512485.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11237..11710)
                     /gene="creA"
                     /locus_tag="ACHKW5_003136"
     CDS             complement(11237..11710)
                     /gene="creA"
                     /locus_tag="ACHKW5_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO7512486.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11921..12790
                     /gene="robA"
                     /locus_tag="ACHKW5_003137"
     CDS             11921..12790
                     /gene="robA"
                     /locus_tag="ACHKW5_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO7512487.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12787..13434)
                     /gene="gpmB"
                     /locus_tag="ACHKW5_003138"
     CDS             complement(12787..13434)
                     /gene="gpmB"
                     /locus_tag="ACHKW5_003138"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO7512488.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13486..14001
                     /gene="yjjX"
                     /locus_tag="ACHKW5_003139"
     CDS             13486..14001
                     /gene="yjjX"
                     /locus_tag="ACHKW5_003139"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO7512489.1"
                     /translation="MHQVVCATTNPAKIQSILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            complement(13995..14321)
                     /gene="trpR"
                     /locus_tag="ACHKW5_003140"
     CDS             complement(13995..14321)
                     /gene="trpR"
                     /locus_tag="ACHKW5_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO7512490.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(14411..16348)
                     /gene="sltY"
                     /locus_tag="ACHKW5_003141"
     CDS             complement(14411..16348)
                     /gene="sltY"
                     /locus_tag="ACHKW5_003141"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO7512491.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPATTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16559..18226
                     /gene="ettA"
                     /locus_tag="ACHKW5_003142"
     CDS             16559..18226
                     /gene="ettA"
                     /locus_tag="ACHKW5_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO7512492.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18282..18566)
                     /gene="nadS"
                     /locus_tag="ACHKW5_003143"
     CDS             complement(18282..18566)
                     /gene="nadS"
                     /locus_tag="ACHKW5_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO7512493.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(18568..18900)
                     /locus_tag="ACHKW5_003144"
     CDS             complement(18568..18900)
                     /locus_tag="ACHKW5_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7512494.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEV"
     gene            complement(18992..20224)
                     /gene="nadR"
                     /locus_tag="ACHKW5_003145"
     CDS             complement(18992..20224)
                     /gene="nadR"
                     /locus_tag="ACHKW5_003145"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO7512495.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(20245..21627)
                     /gene="radA"
                     /locus_tag="ACHKW5_003146"
     CDS             complement(20245..21627)
                     /gene="radA"
                     /locus_tag="ACHKW5_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO7512496.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21676..22644)
                     /gene="serB"
                     /locus_tag="ACHKW5_003147"
     CDS             complement(21676..22644)
                     /gene="serB"
                     /locus_tag="ACHKW5_003147"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO7512497.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRSPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LLMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            22750..23394
                     /gene="ytjB"
                     /locus_tag="ACHKW5_003148"
     CDS             22750..23394
                     /gene="ytjB"
                     /locus_tag="ACHKW5_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO7512498.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23422..24438
                     /gene="lplA"
                     /locus_tag="ACHKW5_003149"
     CDS             23422..24438
                     /gene="lplA"
                     /locus_tag="ACHKW5_003149"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO7512499.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAIREAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            24470..>24730
                     /locus_tag="ACHKW5_003150"
     CDS             24470..>24730
                     /locus_tag="ACHKW5_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7512500.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
BASE COUNT         5929 a         6816 c         6112 g         5873 t
ORIGIN      
        1 agcgtcgcat caggcgctga atgccggatt gttacccggc atttgtcatt tagcggtgat
       61 gtttgcctgg accatgaccg ccgtgatgat catgatgagc tttcttatgg tggcgcggcg
      121 gtggcggcgg tccgtgtggg tgccagcgat tgcctcgcca ttcataatgt tgtttccacc
      181 agccgtggtc gcgccagtga ccgccatccc agtaataacc gcgattatca cgatcgccta
      241 tctgtaattt tactgacggg actaacgtaa tttccgcagc ctgcgctgcc atgggagcga
      301 ccagaaccag ggaaagtgcg agtacgatag attgcatctt tttcacaggt tactcctttt
      361 atcattgccc gttatgggtc gatatccgga gattacgcga tgagaacggc ggttgttatt
      421 ctccgcaatc ctaatctcta agttaccgca tttattgaga atttctcctt tttccctgtc
      481 gtttttctcc ataatttctt cataaaaaag ccgggctgca taaaagcaaa cccggcctga
      541 ttgagataat gaatagattt tactgatgat tcatcatcaa tttacgcaac gcagcaaaat
      601 cggcgggcag attatgtgaa agcaagggta aatcagcacg ttctgccagc tcttttggca
      661 gatccaacgt ttcaccgaga atcgattcca cgctctcttt aaatttcgcc ggatgcgcgg
      721 tgccgaggaa caagccatat tcgcctggat tcaactggtc acgcagcgca cgatacgcta
      781 cggcagcgtg cggctccgag gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg
      841 tggtttcatc atccacggct gcataaccca gctctttcag ttgccagatt ttgcggcgga
      901 acaactcttc cacacgcggc cagttgttcg gctgactcac gtccatcgcg ttggataacg
      961 tcgcctgagt cgctttgggt gaccactgac cgtcgtgcag gaaacgtggc acggtatcgt
     1021 tcacgttggt cgcagcaata aaacgtttca ccggcagacc gagtgacttc gccagcagac
     1081 ccgccgtcaa atcgccgaag tttccgcttg gcaccgagac aaccagctgg ttgcgcgctt
     1141 cctgtggcag ctgcgcaaca gcttcaaagt agtagcaaat ctgcgccagc aaacggctga
     1201 tgttaatcga gttagctgag tttaacccca gcgccacttt cagttcttca tcatcaaacg
     1261 cctgcttcac cagcgcctga caggcatcga aatcgccgtc gatggcaaca gtttcgatat
     1321 tgccgcccaa cgtacagaac agtttttctt gcagtggact gattttgcct cgtggataga
     1381 ggataaccac tttcacattc ggtaaaccgt agaaagcatg agccactgcc gctccggtat
     1441 caccggaggt cgcggtcaga atggtcactg gcttatcgcc cgcaatatgg gtcagcattt
     1501 gtgccataaa gcgaccgccg aaatctttaa atgccagcgt tggcccgtgg aacaattcca
     1561 gacaaccgac atcgctttca acattggcga ccggagccgg gaaggcaaac gccgcgcgca
     1621 cgcgctcttc caggatttcc tgcgggattt catcaccaat aaacgccgag aggatcttcg
     1681 cactgcgggt gacaaaatcc agcttcagca tctcatcaat ttcagtcagg ctgaattccg
     1741 gcaggtcatg cggaaaaaac agcccctgat ttttgcccaa cccctgggtt acggcttgcg
     1801 caaagctgac ctgctcgttg tgatctttca gattgtagag tttcatttag ttttccagta
     1861 ctcgtgcgcc cgccgtatcc agccggcaaa tatgaacaaa accttcctga ttttgcaggt
     1921 agttcttacc caaccagtcg gcaacgcgct gggcggtatc cggcttgtca catagagcga
     1981 acaaggtcgg gccggagccg gagataccgc tcgctaccgc gccgatttcc gcgaccgcct
     2041 gccgcgcctg ccggaagcca ggcagtaacc gttcacggta gggttcagcg ataacatctt
     2101 tcatcagctt cgcggcaagc tcaggctgac gggaatagca ggcgtgaatg aagccagcca
     2161 gatgtcgccc gtgcgcaatg caatcctggc ggcgatactg cgccggtaaa atagccctgg
     2221 cttctgccgt cgagacttta atccccggat acgccagcac ccacagccac tcatcaaacc
     2281 ctggcacttg ctggctgatg atgtcgtttt cttcgatcat caactgcata ccaccaagaa
     2341 aacacggtgc cacgttgtcg taatgaatgc tgccggagat tcgtccttcc agctcgccca
     2401 tcaaagccag caaacgagtg tcattaagcg gcttgccaca gtgttcattc atcgccatca
     2461 gcgccgcgac caccgaacag gcgctggagc ctaagcccga accgattggc atattctttt
     2521 ccagagtcat cgccactgga atttgcttgc caagctcctg gcaaaaacgc tcccagcact
     2581 gataaacgat attttcccgt ggttctgacg gcagcttatc ggcaaagcgt ccgaggttgt
     2641 tgagactgaa tgtctctgcc gcctcaaccg tgactacatc tccgagcaat gcaccatcaa
     2701 caggtgtcac cgccgccccg agcacatcaa acccgacgct catattggca ctggaagccg
     2761 gggcataaac tttaaccatg tcagactcct aacttccatg agagggtacg tagcagatcg
     2821 gcaaagacac cggcagctgt aacgtcattg cccgcaccat atccgcgcag caccaacggc
     2881 agcggctgat aatagtggct ataaaaggcc agggcgtttt cgccattttt cactttgaac
     2941 agcggatcat taccatccac ttcggcaatc ttcacgcggc aggcaccatc ttcatcaata
     3001 ttgccaacat agcgcaaaac ttttccttca tcacgggcct tcgccacgcg cgcggcaaag
     3061 agatcgtcga gctgtgacag attcgccata aaagcggcaa catcaccctc agcgttaaac
     3121 tctgcgggca gcacaggttc aatttcaata tccgccagct ccagttcacg tcccgtttca
     3181 cgagcgagaa tcaatagctt acgcgctaca tccataccag aaagatcatc tcgcggatcc
     3241 ggttcggtat aacccatttc ccgcgccaga gtagtcgcct cggagaaact catgccttcg
     3301 tctaacttgc cgaagatata agaaagcgaa cctgaaagaa tgccggagaa cttcatcaat
     3361 tcatcaccag cattgagcag attttgcagg ttctcaataa ccggtaatcc agccccaacg
     3421 ttggtgtcat agaggaattt acgccgcgat ttttccgccg catgacgcaa cagatggtag
     3481 taatccatcg acgaggtgtt ggcctttttg ttcggcgtga caacgtggaa accttcgcgc
     3541 aagaagtcgg catattgatc cgccactgcc tggctggagg tgcagtcaac aatgaccggg
     3601 ttcagcagat gatattcttt cacgaggcga attaagcgcc cgagattaaa cggctctttg
     3661 gcttgcgcca gttcttcctg ccagttttcc agatttaggc catgcacatt ggtgagcagt
     3721 gccttcgagt tggcaacacc gcagacacgt aagtcgatat gtttattctt cagccagctt
     3781 tgttgacgct tcagttgctc cagcagcgca ccgccaacgc caccgacgcc aatcacaaac
     3841 acttcgataa cctgatcggt attgaacagc atctgatgag taacgcgcac gccagtggtc
     3901 gcatcatcgt tatttaccac gacagagatt gagcgttcag aagatccctg agcaatggcg
     3961 acaatgttga tattggcgcg ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag
     4021 gtgcgcatac catcacctac caccgagata atggccagcc gttccgtcac cgccagcggc
     4081 tccagtaagc cttctttcag ttccaggtag aactcttcct gcattgcccg ttcagctcgc
     4141 acacagtcgc tttgtggaac gcagaaactg atactgtatt cggaagatga ttgcgtaatc
     4201 agcaccacgg aaatacgggc gcgtgacatc gcggcaaaga cgcgcgccgc catgccgacc
     4261 attcctttca tccccgggcc ggaaacgctg aacattgcca tgttattcag attggaaatg
     4321 cccttgaccg gtaattcgtc ttcatcacga ctggcaccaa tgagcgtacc tggtgcttga
     4381 ggatttccgg tatttttaat caggcaaggg atctggaact gggcgatggg ggtaatggtg
     4441 cgggggtgaa gaactttagc gccgaagtag gaaagctcca tcgcttcctg gtaggacatc
     4501 gacttcaaca acctcgcatc gggcacctga cgcgggtcgc aggtatagac cccgtcaacg
     4561 tccgtccaaa tctcgcaaca atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag
     4621 tcggaaccgt tacgtccaag taccaccagt tcgccttttt cattaccggc ggtgaagcct
     4681 gccatcagca ccatgtgatc agccggaata cgacttgccg caatacggcg ggtggactca
     4741 gcaatatcga cggtagattc gaggtaatgc cccactgcca gcagtttttc gaccggatcg
     4801 ataacggtaa cgttgtgacc acgcgcttct aacacgccgg ccataatggc gatcgacatt
     4861 ttctcgccac ggcaaatcag cgcagcgttg atgctatccg ggcactgccc caacaaacta
     4921 atgccatgca ggacatgttt tatttgggca aattcctgat cgacgaaagt tttcaattgc
     4981 gccagcggga accccggctg ggcggcggcg agtcccgtca aaagttcggc aaaaatacgt
     5041 tcggcatcgc tgatattggg taaagcatcc tggccgctaa tggttttttc aatcatcgcc
     5101 accaggtggt tggtgatttt ggcgggggca gagaggacgg tggccacctg cccctgcctg
     5161 gcattgcttt ccagaatatc ggcaacacgc agaaaacgtt ctgcatttgc cactgatgta
     5221 ccgccgaact tcaacactcg catggttgtt acctcgttac ctttggtcga aaaaaaagcc
     5281 cgcactgtca ggtgcgggct tttttctgtg tttcctgtac gcgtcagccc gcaccgttac
     5341 ctgtggtaat ggtgatggtg gtggtaatgg tggtgctaat gcgtttcatg gatgttgtgt
     5401 actctgtaat ttttatctgt ctgtgcgcta tgcctatatt ggttaaagta tttagtgacc
     5461 taagtcaata aaattttaat ttactcacgg caggtaacca gttcagaagc tgctatcaga
     5521 cactcttttt ttaatccaca cagagacata ttgcccgttg cagtcagaat gaaaagctga
     5581 aaaatactta ctaaggcgtt ttttatttgg tgatattttt ttcaatatca tgcagcaaac
     5641 ggtgcaacat tgccgtgtct cgttgctcta aaagccccag gcgttgttgt aaccagtcga
     5701 ccagttttat gtcatctgcc actgccagag tcgtcagcaa tgccatgact cgttcgcgta
     5761 aagcttgcag ttgatgttgg tctgccgttg tgtcactttt cgccggttgt tgtattaatg
     5821 ttgctaattg atagcaatag accatcaccg cctgccccag attgagcgaa ggataatccg
     5881 ccaccatcgg cacaccagta agaacgtcag ccaacgctaa ctcttcgtta gtcagcccgg
     5941 aatcttcgcg accaaacacc agcgcggcat ggctcatcca tgaagatttt tcctctaaca
     6001 gcggcaccag ttctactggc gtggcgtagt aatgatattt cgcccgactg cgcgcagtgg
     6061 tggcgacagt gaaatcgaca tcgtgtaacg attcaaccag tgtcgggaaa actttaatat
     6121 tatcaataat atcaccagat ccatgtgcga cccagcgggt ggctggctcc agatgtgcct
     6181 gactatcgac aatccgcagt tcgctaaaac ccatcgtttt cattgcccgc gccgctgccc
     6241 caatattttc tgctctggcg ggtgcgacca gaataatcgt tatacgcata ttgccactct
     6301 tcttgaccaa ataaccgcga acagggtgat cactgtcaac ttattacgcg gtgcgaattt
     6361 acaaattctt aacgtaagtc gcagaaaaag ccctttactt agcttaaaaa aggctaaact
     6421 atttcctgac tgtactaacg gttgaattgt taaaaaatgc tacatatcct tctgtttact
     6481 taggataatt ttataaaaaa taaatctcga caattggatt caccacgttt attagttgta
     6541 tgatgcaact agttggatta ttaaaataat gtgacgaaag ctagcattta gatacgatga
     6601 tttcatcaaa ctgttaacgt gctacaattg aacttgatat atgtcaacga agcgtagttt
     6661 tattgggtgt ccggcccctc ttagcctgtt atgttgctgt taaaatggtt aggatgacag
     6721 ccgtttttga cactgtcggg tcctgaggga aagtacccac gaccaagcta atgatgttgt
     6781 tgacgttgat ggaaagtgca tcaagaacgc aattacgtac tttagtcatg ttacgccgat
     6841 catgttaatt tgcagcatgc atcaggcagg tcagggactt ttgtacttcc tgtttcgatt
     6901 tagttggcaa tttaggtagc aaacatgcag accccgcaca ttcttatcgt tgaagacgag
     6961 ttggtaacac gcaacacgtt gaaaagtatt ttcgaagcgg aaggctatga tgttttcgaa
     7021 gcgacagatg gcgcggaaat gcatcagatc ctctctgaat atgacatcaa cctggtgatc
     7081 atggatatca atctgccggg taagaacggt cttctgttag cgcgtgaact gcgcgagcag
     7141 gcgaatgttg cgttgatgtt cctgactggc cgtgacaacg aagtcgataa aattctcggc
     7201 ctcgaaatcg gtgcagatga ctacatcacc aaaccgttca acccgcgtga actgacgatt
     7261 cgtgcacgca acctgctgtc ccgtaccatg aatctgggta ctgtcagcga agaacgtcgt
     7321 agcgttgaaa gctacaagtt caatggttgg gaactggaca tcaacagccg ttcgttgatc
     7381 ggccctgatg gcgagcagta caagctgccg cgcagcgagt tccgcgccat gcttcacttc
     7441 tgtgaaaacc caggcaaaat tcagtctcgt gctgaactgc tgaagaaaat gaccggccgt
     7501 gagctgaaac cacacgaccg tactgtagac gtgacgatcc gccgtattcg taaacatttc
     7561 gaatctacgc cggatacgcc ggaaatcatc gccaccatcc acggtgaagg ttatcgcttc
     7621 tgtggtgatc tggaagatta atcggcttta ccaccgtcaa aaaaaacggc gctttttagc
     7681 gccgttttta tttttcaacc ttatttccag atacgtaatt gatcgtccat tgtaacttct
     7741 ttactggctt tcattttcgg cagtgaaaac gcataccagt cgatattacg agtcacaaac
     7801 atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca
     7861 gagttgagca gtccccacat cacaccatcc agcaacaaca gcgcgagggt aaacaacatg
     7921 ctgttgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc
     7981 agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc cagcaagagc
     8041 aaataaaaca ttaccaatga aagccccacc agcaaatatt gcattgggtg taaacgttgc
     8101 gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag cagaatggcg
     8161 tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac tgcgacgcta
     8221 aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta
     8281 ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctaac ttcccgtttg
     8341 gctggcaaaa aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg
     8401 ctattacgcc cggcaggcac cacagaaaga tcgccggtac cgcttaaatt cagggccata
     8461 ttcagcttca ggttctgctt ccgccagtcc ccttcgggta aagggatatg cacgccctgc
     8521 ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt aacttcaggc
     8581 gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa gggcttgcct
     8641 aagatgatat ttggcgcatt gagctcgcta agacgcgaaa catcgaaatc ggcttttagc
     8701 gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttccacgttc
     8761 tgattgccat caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc
     8821 tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat agcgataagc
     8881 ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta
     8941 cggtaatcgg cacgttcgac aatcacctgc cgaatcagca ttatcggaat caacaacagc
     9001 aatactgcac caaacagggt ggtcattttc cagaacaggg gggatttcaa catagtcttc
     9061 tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag
     9121 tgacggtgaa gtcgaagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact
     9181 tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt
     9241 tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca
     9301 ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc cacttcggcg
     9361 cttagcgtta tacgaccgct ctcgggggta aaatcgatgg cgttatccag taaattcccc
     9421 agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggtggg cgtaacatgt
     9481 agagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcggaataat
     9541 gccgccacat caacagcagt caaaacgact tcctgacgat tctccagtct tgcctggcgt
     9601 agtaacgttt ccaccagtgc ctgcattcgc gcattttgcg tcagaatgtt gtcagtaaaa
     9661 cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc
     9721 gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc
     9781 ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag ctcgctacta
     9841 ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata gcgagtgagc
     9901 cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcgatcccc
     9961 aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac cggagccatc
    10021 gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc cataatcggc
    10081 gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca
    10141 ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgcccaacg
    10201 gctttatttg ccgaatcgaa caatacttta ccctgcgaat cggtcatata gacatgatat
    10261 tcattgcgca ctttgttaat gccaccgata ttggcgcgaa acgggcgatg ttgtagctga
    10321 ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc
    10381 agctccgcca gcaacgttgc ggtgtcgatc aacgtcccct ccgttgctct tcgcacgccc
    10441 ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa
    10501 aaatagccca gcagcaaccg catgccgata cgcattacag gcccctcagg ctatatccca
    10561 tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca
    10621 acgttttaat gtgggtatca acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc
    10681 tatccatcag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca
    10741 ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg
    10801 gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgacgag aacttcttca
    10861 cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca
    10921 catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg
    10981 tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc
    11041 cactgatatc aggcaaacca acgtcaagaa tcatgacgtc gggaacctgc tggcgagctt
    11101 tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca
    11161 tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt
    11221 gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt
    11281 tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg
    11341 cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata
    11401 ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt
    11461 gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc cctttaatac
    11521 caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg
    11581 cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa
    11641 tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct
    11701 tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa
    11761 taacatattg attaagaaca aaatctcaat acttttattt ccgtcaagcc ctaaaacata
    11821 ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc
    11881 tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat
    11941 tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt
    12001 agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg
    12061 ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg
    12121 cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac
    12181 atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc
    12241 tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca caatgccaga
    12301 gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg
    12361 ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt
    12421 tctcggcaac gcgccgacca ttccgccagt gctctacggc ctgaatgaaa cgcgtccgag
    12481 tcaggataaa gacgacgaac aagaggtatt ctataccact gcgttagccc aggatcaggc
    12541 agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt
    12601 tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacagtat acggaacgtg
    12661 catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat actacccggc
    12721 agaagatgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattccgat
    12781 ccgtcgttaa cgctgcaact catctaatgc aggggcgtcc agatgcgaga tgtcccctgc
    12841 ggtttcaaca acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat
    12901 agaacagtta cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact
    12961 caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc cctgcggtaa
    13021 gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc
    13081 ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc
    13141 ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg
    13201 agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt
    13261 gcgtcctaaa tcgctactga tgatatgcgt aatgccaagc tctttggcac gggttgccac
    13321 ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc cctgaatacg
    13381 tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt
    13441 tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc
    13501 tgtgcgacca ccaatcccgc taaaattcag tccattctgc aggcatttca cgagatcttc
    13561 ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc ggaacaaccc
    13621 tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta
    13681 cttccagagg ctgatttttg ggtggcgatt gaagctggca tcgatggtga cagcactttc
    13741 agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc tactttgccg
    13801 ttgcctgcgg tgattctgga aaaagtgcgt gaaggtgagg cacttggtcc cgtgatgtcg
    13861 cgttataccg gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc
    13921 gggaaactca ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt
    13981 cataatgcgg tgtatcagtg atctttcagc aacacctctt ccagccactg gcgcagctcg
    14041 acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg tcgcgatgcc agcgccgagt
    14101 tcatttttca actcacgctg gctcatttcg ccgcgcaaca gctcttcgac aatacgcacg
    14161 cgagtcccca acgcttcgcg ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg
    14221 agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc
    14281 tgttctgcca tcgctgctga atagggtgat tgttgggcca taatatgtcg ccattgttag
    14341 cgggggaagc aaaatgcctc ccccggttgt actcgctaaa gagtacgata gcatatcata
    14401 aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct catcaatgtc ggtttatccc
    14461 ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt cttcacataa ccgcgcgtct
    14521 cggagaacgg aatactctcg acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa
    14581 gccaggttcg cacccgccct ggtccggcgt tataagctgc tgaggagaaa atacggttat
    14641 taccaaactg ctgataaaca tattgcaggt aactggtgcc aatgttgatg tttgtttccg
    14701 gatccagcaa ttgcccaggg ctgctataac cgggaataga gaacatcttc accgtatggg
    14761 tcgccgtgcc cggcataatc tgcatcaggc cgctggcccc taccggcgat ttcactttcg
    14821 gattccaggc actctcctga cgggcaatcg ccatcgcata gctttgcggg atctccttcc
    14881 cactggtgta acgtttgaaa agatcgttgt aggccagcgg gaatctctct tccagatgat
    14941 cccaaagctt cccggcgatc gttgcctgaa cgctaagatc ccaccattgg ttattgaaag
    15001 cataccgagc cagttgcgcc tgctctgttt ttgacttgct cttcaccaga ttggcccact
    15061 cgctacgcgc ggtgttatcg agattccagt acatcaactc gcgcacgcgc gccatctccg
    15121 gcccctgagt cagggcgctg tcaacattct gcggcgcttt atcaatcttc agctcatact
    15181 cttcgccgat gcgctgtgca gcaaccatcg ggtagaaacc acgctgttgc atgagttgat
    15241 gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag cagtaaatcc gcctgccagt
    15301 aacgccattc atctttctct ttcgcttcca tcggcagacg cgccagccag gtattcaggc
    15361 cgcgacgatc gccggtgcca agcgccattc gcacacggcg ttcaataagc gaagtagatt
    15421 gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc gtcggtgaca tcgttgccca
    15481 tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg atcttcatta agctgctgtg
    15541 cctgagcaag tgacgggatc atcagccgtg cattttcagc atcctgccgc gccacgctgg
    15601 caaacgccac cgccgccatt tgacgggtaa aatcggtcgc accggttgta cgcgcgaagg
    15661 tcagtaccgt attagggttg ttcgccagag aaataatcgc cgaggcgata gtctgataat
    15721 cagcaggcat ctgacctgcc agcactgtca ccagacccgt attacccgct ttcatcgcca
    15781 gacggatacg ctctaaatac gccagcggat cttgtttacc tgacgcacgc cagacactaa
    15841 ataacttgtc acaggcgtta ggctggctct tgccggttag ccacagctct ttcgcccctt
    15901 gccaggcttc ttcactctgc ccggtgttcc atttcgcata gtagtaatta cattgtgctt
    15961 cggtagttgc gggcttttcc gggctaaagg ctaacaggcc acgccagtct tcacgccgcg
    16021 ccagttcatt gacgaaacga gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc
    16081 gaacaaagtt agtgaccgtc accgccggtt gattcatcag atcatcggtg atctggcggt
    16141 attccagata gggataaagt ggataatcct tcaatccagg catcatttgt tcgaccacat
    16201 ccatttgtcg attatcccag gcctgcttaa tttgcgcgta acggctacgc tgctcatcca
    16261 gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca gacaccgaca gccaacagcc
    16321 gccaggtaac gtgtttggct ttttccacaa gcgcatcctc taagtgtcaa tacatcaatg
    16381 cagcgtcatg ccgcgtaata gatttatgct aaccagtcat tgccgtttac gccacgttac
    16441 ggacactttt ttacttttac tgcgagggtg atcgaccggc tgggattaga taaggaaaaa
    16501 ggctacactt cgcctttgaa aactcatcat cacgataaac atagaggcga agtccaacgt
    16561 ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat
    16621 tttgaaaaac atctctctga gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa
    16681 cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc attgataaag atatcgaagg
    16741 tgaagcgcgt ccgcagccag acatcaagat tggttacctg ccgcaggaac ctcagctgaa
    16801 cccggaacac accgtgcgtg agtccattga agaagcggtt tctgaagtgg ttaacgctct
    16861 gaaacgtctg gatgaagtgt atgcgctgta cgccgatccg gatgccgatt ttgacaagct
    16921 ggccgctgaa caaggccgtc tggaagagat cattcaggct catgacggtc ataacctgaa
    16981 cgttcagctg gagcgtgcgg ctgatgcgct gcgtctgccg gactgggacg cgaaaatcgc
    17041 taacctttcc ggtggtgagc gtcgtcgcgt ggcgttgtgc cgcctgctgt tggaaaaacc
    17101 agacatgctg ctgctcgacg aaccgaccaa ccacctggat gccgaatccg tagcgtggct
    17161 ggaacgcttc ctgcacgact tcgagggtac cgtggtggcg attacccacg accgttactt
    17221 cctcgataac gttgcaggct ggatcctcga acttgaccgc ggtgaaggta ttccgtggga
    17281 aggcaactac tcctcctggc tggagcagaa agatcagcgc ctggcgcagg aagcttcaca
    17341 agaagcggcg cgtcgtaagt cgattgagaa agagctggag tgggtacgtc agggaactaa
    17401 aggccgccag tcgaaaggta aagcccgtct ggcgcgcttt gaagagctga acagcaccga
    17461 atatcagaaa cgtaacgaaa ccaacgaact gtttattcca cctggaccac gtctgggcga
    17521 caaagtgctg gaagtcagca acctgcgtaa atcctatggc gatcgtctgc tgattgatga
    17581 cctgagtttc tcgatcccga aaggggcgat cgtcgggatc atcggtccga acggtgcggg
    17641 taaatcgacc ctgttccgta tgatctctgg tcaggaacag ccggacagcg gcaccatcac
    17701 tttgggtgaa acggtgaaac tggcatcggt tgatcagttc cgtgactcaa tggataacag
    17761 caaaaccgtt tgggaagaag tttccggcgg gctggatatc atgaagatcg gcaacaccga
    17821 gatgccaagc cgcgcctacg ttggccgctt taactttaaa ggggttgatc agggtaaacg
    17881 cgttggcgaa ctctccggcg gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt
    17941 tggcggcaac atgctgctgc tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg
    18001 cgcgctggaa aacgccctgc tggagttccc gggctgtgcg atggttatct cgcacgaccg
    18061 ttggttcctc gaccgtatcg ccacgcacat cctggactac caggatgaag gtaaagttga
    18121 gttctttgaa ggtaacttta ccgagtacga agagtacaag aaacgcacgc tgggcgcaga
    18181 cgcgctggag ccgaagcgta tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg
    18241 gatgcggcgc gccagcatcg catccggtaa tagttaaatg attaatggga ggcaagttcc
    18301 cggaaaaagg caggattttc tttaattgtg gcaagtactt ttgcagccaa tcccgtagga
    18361 tttcttcgct tcgattccca gcttttaatg gtatctacac tggttcccag agcttttgcc
    18421 atctcacttt gagaaacgtt gagttgctca cgaatagctt taacatccgc aatttcgtag
    18481 cgggtcaccc gtgcaggttc ttttaaacct tgtttaatct cgacagcctc ttccagagag
    18541 gttttcaact catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcgtcaactt
    18601 ctttagttga gctttttccg tatctgtcag actatccttg gcatttttcg gataggccat
    18661 aatcaaataa ataatctctt ccgtcgccag aaagtaaatt attctgacac tgccactttt
    18721 tccctgtgaa cccgcagcca ttcggacttt acgcaatcca ccagtctgct gaatgagatc
    18781 acctttatcg ggagttccga tgagttcttt ttgtaattcc tttaattcat catcggttgc
    18841 aatctgcttg atctgacgcg taaacattgg cgtctctata aatattatcg ccttacccat
    18901 tacccctccc tggcaggtat attgtacacc gaaatggagt actaagtaca cttttgaatc
    18961 agtaatccca ctcaacatct tataaagatg attatctttg ctcccccatc atctcccgca
    19021 ccagctccac gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacaaatt
    19081 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    19141 aactgccgag actgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    19201 ccagatcaaa acggtattca tcgatcagcg cctgtacgaa cggatgctcg cgcccttcgt
    19261 actttttgca gaacgcctga gttgtcacaa aatcggtgtc gataaacgcc actttattgg
    19321 catattttac tgcaaaatca atatattgag cgtgtcccag cgcgatttta tcgtagtcgg
    19381 aatactgcaa tgcaatctca tcgccgccaa gatgcgaaaa gacataatcg cgaccatatt
    19441 cccatgcact ggtagtgttg aagatattag caagtttgtt taccagggtg gatttaccgc
    19501 tcgactcgcc gccaaggatc gccacggtgc gcacaaagaa cggcttcact tcggtaggaa
    19561 tatattccca gtagcggaat ggattttcgc ggatctgcgc accgctaata ctcataaagg
    19621 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    19681 cggcttcttc cgaggtgtag atcagatccg gctggatccc tttttcagcc ataaactttt
    19741 tgatgccgtt gctccacaca tcccagccgt gcggatacgg ctccatgccc tcttcgttga
    19801 aggcatgaat acggatattt ttctgatatt taaaggtttg caataaccag cgcagacgat
    19861 ccggcaccgt tggctgctgc gacatggcgc tgtcttcaaa caacgcacga tcgcgggtat
    19921 cgtcaaagcc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    19981 agatatgtcc ggtatgcaac gggtagaact taccgaatac cacgccaatg gttttctttt
    20041 ggcggggaaa ctcaagcccc aaaaaacggt gcaacgcctc cagtttttgc gcgctggggc
    20101 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    20161 ctacctgctg tagcgtgcag ccctgttgct taatggcagt tttcaggtaa tcaaatgacg
    20221 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    20281 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    20341 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagag attcgttcct gcccgctggg
    20401 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    20461 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc
    20521 tgtcaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    20581 accgtgacgg tgcaagacag ccagcaggat cgccagacgg ttttgctcca aacctaccgc
    20641 cacgcggcgt ggattcgcca tcatcgagtg atcgaccagc gcctggatct ccaccagcag
    20701 cggacgcgtt ccttcccaca ctaccatcac tgagctaccc gaggtcactt catcaccgcg
    20761 gcttaaaaag attgccgaag ggttgctgac ttcacgcaga ccctgttcgg tcattgcgaa
    20821 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    20881 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg
    20941 gccagccagc gagccatctt tggttacgtg cccaaccatg acaatcgcca cgccgcgcgt
    21001 tttggcgaag cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc tgccaggcga
    21061 tgactgaaca tccgccatat gcatcacctg gatggagtca attaccatca gcttcggttg
    21121 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    21181 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    21241 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca acagcagcgt
    21301 ggatttcccc gcacctgggt taccgccaat cagaatggcg cttcccggca ccaccccgcc
    21361 gcctaatacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    21421 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cactgagacg
    21481 ctcgttacgc gccaccgttg gcgacgctgc aagacgcacc tcggtgatgg tgttccaggc
    21541 atgacaggcg ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    21601 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    21661 atgacgggcg agcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    21721 caggtcagcg tgacggatgg tgacttccgt cttttcattc acttttggct tggcatggta
    21781 ggcaatcccc agccctgccg ctttgatcat cagcaggtca ttggctccat cgccaatcgc
    21841 cacggtttgc gccagcggga tttcatactc ctgcgcgagg cgagtcagtg ttttcgcttt
    21901 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    21961 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    22021 agtaaagcca ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagtaccag
    22081 ttgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    22141 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    22201 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc
    22261 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggaga
    22321 gcgcaggtgc gggattttcc ccaacggcgc gacatccagt tgcgcttcgt gggccaggcg
    22381 cgtagcccgc gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    22441 ggcggcaaca atcaccatag ccgcgcccag tttgctctgg tattgggtca gacgttgttt
    22501 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    22561 cacttcatca ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag
    22621 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    22681 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    22741 gggtggaaga tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg
    22801 ttctgtctcg ccttgttagt ggcgctgatg cagggggcgt catggtttag tcaaaaccac
    22861 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg
    22921 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgtat tcaggcgatc
    22981 cttgatcagt taacggatga aagccgcatc ctcgacgcgg gcgtgtatga cgaacaaggc
    23041 gatcttatcg cccgttctgg cgaaagcgtc gaagtgcgcg accggttggc gctcgacggt
    23101 aaaaaagcgg gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    23161 ccgctcggct atctgcgcct gacgctcgac acccatacgc tcgccaccga agcccaacag
    23221 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcgctggc aatcggcgta
    23281 gtgctgaccc gtacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    23341 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    23401 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    23461 ccgtggttta acctggcggt ggaagagtgt atttttcgcc agatgcccgc cacgcagcgc
    23521 gttctgtttc tctggcgcaa tgccgacacc gtggtgattg gtcgcgcaca gaacccgtgg
    23581 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagcagc
    23641 ggtggcggcg cggtgttcca cgatctcggt aatacctgct ttacctttat ggctggcaag
    23701 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgctctgaa cgcgctcggc
    23761 gtcagcgccg aagcgtccgg ccgtaacgat ctggtggtga aaaccgccga aggcgaccgc
    23821 aaagtctcag gctcagccta tcgcgaaact aaagatcgcg gcttccatca tggcaccttg
    23881 ctactcaatg ctgaccttag ccgtctggca aactatctca acccggataa aaagaaactg
    23941 gcggcgaaag gcattacgtc ggtacgttcc cgcgtgacca atctgaccga attgctgccg
    24001 gggatcaccc atgagcaggt ttgcgaggcg attcgagagg cctttttcgc ccattatggc
    24061 gagcgcgtgg aagcggaaat catatccccg gacaaaacgc cagacttgcc gaactttgcc
    24121 gaaacctttg cccgccagag tagctgggaa tggaacttcg gtcaggctcc ggcgttctca
    24181 catctgctgg atgaacgctt cacctggggc ggcgtagaac tgcatttcga cgttgaaaaa
    24241 ggccatatca cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc
    24301 ctcgccgggc gactgcaagg ctgcctgtac cgcgcagata tgctgcaaca ggagtgcgaa
    24361 gcgctgttgg ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata
    24421 gcgggggcgg taaggtaact cactgattta aatatccagg gaaggaaata tgatcgatct
    24481 ggaaaaccag gaacgggaga ttatcaattt aatgttcagc cagggtatat cctggctaac
    24541 cgccgtgcgt atcaggcata aactatcact tgctgaagta agtaaaatgt taggtatctc
    24601 cattaattct ctaaaacaga tcgaaaagac ggaacggctt agttcaaaca tcaaaagcaa
    24661 aatggcagga atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc
    24721 tgaacataaa
//
LOCUS       DAZFYC010000072        24162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFYC010000072 DAZFYC010000000
VERSION     DAZFYC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.072"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="ACHKW5_003151"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="ACHKW5_003151"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO7512501.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="ACHKW5_003152"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="ACHKW5_003152"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO7512502.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="ACHKW5_003153"
     tRNA            2652..2727
                     /locus_tag="ACHKW5_003153"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            complement(2854..3192)
                     /locus_tag="ACHKW5_003154"
                     /pseudo
     CDS             complement(2854..3192)
                     /locus_tag="ACHKW5_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(3243..4199)
                     /gene="paoD"
                     /locus_tag="ACHKW5_003155"
     CDS             complement(3243..4199)
                     /gene="paoD"
                     /locus_tag="ACHKW5_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO7512503.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(4209..6407)
                     /gene="paoC"
                     /locus_tag="ACHKW5_003156"
     CDS             complement(4209..6407)
                     /gene="paoC"
                     /locus_tag="ACHKW5_003156"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO7512504.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            complement(6404..7360)
                     /gene="paoB"
                     /locus_tag="ACHKW5_003157"
     CDS             complement(6404..7360)
                     /gene="paoB"
                     /locus_tag="ACHKW5_003157"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO7512505.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            complement(7357..8046)
                     /gene="paoA"
                     /locus_tag="ACHKW5_003158"
     CDS             complement(7357..8046)
                     /gene="paoA"
                     /locus_tag="ACHKW5_003158"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO7512506.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            8464..9076
                     /gene="yagU"
                     /locus_tag="ACHKW5_003159"
                     /pseudo
     CDS             8464..9076
                     /gene="yagU"
                     /locus_tag="ACHKW5_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
     gene            complement(9324..9653)
                     /gene="ykgJ"
                     /locus_tag="ACHKW5_003160"
     CDS             complement(9324..9653)
                     /gene="ykgJ"
                     /locus_tag="ACHKW5_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7512507.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(9966..10676)
                     /gene="ecpE"
                     /locus_tag="ACHKW5_003161"
     CDS             complement(9966..10676)
                     /gene="ecpE"
                     /locus_tag="ACHKW5_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO7512508.1"
                     /translation="MFRRRGVTLTKALLTAVCMLATPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPECDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            complement(10645..12288)
                     /gene="ecpD"
                     /locus_tag="ACHKW5_003162"
     CDS             complement(10645..12288)
                     /gene="ecpD"
                     /locus_tag="ACHKW5_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO7512509.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            complement(12278..14803)
                     /gene="ecpC"
                     /locus_tag="ACHKW5_003163"
                     /gene_synonym="yagX"
     CDS             complement(12278..14803)
                     /gene="ecpC"
                     /locus_tag="ACHKW5_003163"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO7512510.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDHGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            complement(14829..15497)
                     /gene="ecpB"
                     /locus_tag="ACHKW5_003164"
                     /gene_synonym="yagY"
     CDS             complement(14829..15497)
                     /gene="ecpB"
                     /locus_tag="ACHKW5_003164"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO7512511.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDRQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            complement(15557..16145)
                     /gene="ecpA"
                     /locus_tag="ACHKW5_003165"
                     /gene_synonym="matB"
                     /pseudo
     CDS             complement(15557..16145)
                     /gene="ecpA"
                     /locus_tag="ACHKW5_003165"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
     gene            complement(16220..16762)
                     /gene="ecpR"
                     /locus_tag="ACHKW5_003166"
                     /gene_synonym="matA"
     CDS             complement(16220..16762)
                     /gene="ecpR"
                     /locus_tag="ACHKW5_003166"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO7512512.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            17587..17812
                     /gene="ykgL"
                     /locus_tag="ACHKW5_003167"
                     /pseudo
     CDS             17587..17812
                     /gene="ykgL"
                     /locus_tag="ACHKW5_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(17847..17987)
                     /gene="ykgO"
                     /locus_tag="ACHKW5_003168"
     CDS             complement(17847..17987)
                     /gene="ykgO"
                     /locus_tag="ACHKW5_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO7512513.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(17987..18250)
                     /gene="ykgM"
                     /locus_tag="ACHKW5_003169"
     CDS             complement(17987..18250)
                     /gene="ykgM"
                     /locus_tag="ACHKW5_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO7512514.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(18613..18714)
                     /gene="ykgR"
                     /locus_tag="ACHKW5_003170"
     CDS             complement(18613..18714)
                     /gene="ykgR"
                     /locus_tag="ACHKW5_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO7512515.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(19188..19277)
                     /locus_tag="ACHKW5_003171"
                     /pseudo
     CDS             complement(19188..19277)
                     /locus_tag="ACHKW5_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19830..24083
                     /gene="fdeC"
                     /locus_tag="ACHKW5_003172"
     CDS             19830..24083
                     /gene="fdeC"
                     /locus_tag="ACHKW5_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO7512516.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         5767 a         6015 c         6401 g         5979 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgcttgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg gtacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcagat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag caatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttcttgaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggcgtca gtcgttacaa cagcgatgca ttacgccgta ttgccgggca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagcagg ctgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgtgccaa tggccttggc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgatgat gtgcgccagg tgtgtaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cagcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg
     1741 ctgcaacggt ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc
     2161 agttgcaggc aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccatta aaatcaaaga gttaccccat atttaaatac
     2761 accacgtttc ctcctgtgcc gcatttgtgc cattgtaacc ttggcaattc atcaaaatac
     2821 tgttctgaca tcaggcagtg caggtgcagc tatttaaacc aattgctgcc gccattcttt
     2881 gacgtagtca atcagggcgc ggagctttgg tgcaatattg cgacgctgtg ggaaatacag
     2941 atagaagccc ggaaattgtg gaagaaagtc atcaagcagc gatacaagct taccgctttc
     3001 aatatatggc ctgaaagttt cctgagtggc aattgttatt cctccgccgg caagagccag
     3061 cctcaacatc agacgcagat cattagtcgt aatctgcggt tcaatcgcaa ggtcgaaagt
     3121 tctcccgttt tcttcaaatg gccagcgata aggcgcaacc tccggggact gacgccagcc
     3181 gatacactta tgggtatttc ccccggaggc gagaaagcgc tctccacgcc cggccgcaag
     3241 gatcaggacg acgggggcag gcatgaatcc tcctcctgat ggagacgtac agaggcgact
     3301 tctgccagca cggagagtgc cagagtatgc gcatcccggg ctttggggaa tatcccgacg
     3361 ggtgcccgga tttgcgttgt ttcctccctg gaccatccca gctcgtggag cttttgcaga
     3421 cgtaacgtgt gggttcgata gctgcccaat gcgccgagat aaaagggttt tgcttctcgc
     3481 gcggcctgca acactggcag ctcccggttg agatcatggc acagcaaaat gaccgccgta
     3541 tcggtatcga tctgagcgct ggctgaggcc ggaaaaagat cgaagatatg gctgtcatag
     3601 cctgtggctg ctgcaagact cgcagttgcc tgcgcctcaa gagaacgtcc gtaaatcatc
     3661 agcctgacgc atggcctgaa ccccacctca aagccattga gattccagcc cgttcgggtt
     3721 tgcgtgggca ggcacaccag cgattgtgct tgcggatcgt agcgcagccc cgccggtttt
     3781 ctctgttcca gacggttcag cacggcgagc agaggctgtg ccgagcgtag tttatggagc
     3841 gtcagcgtga tcccaccgcc gcagggcaga acgatgtcaa accacggcga accttcgcca
     3901 tagcgaattt cgcgatcgcg gcctgagccc atcatctcca gcgcttcaaa agcggcagcg
     3961 gcctccacgc agccgccaga gacaaaaccg cagtaacgac catcttcgcg aaccaccatc
     4021 tgcgccccga gcgggcgcgc tgcgccgccg cgtatctcca ccagcgtcac cagcaccgct
     4081 cctttaccgg acattagcgc ctctacggcg aagcgcagaa tggtccggtg gtcatcggtg
     4141 agaaacgcct gctctggctt atgccagtgt tcgtctttat caaaaagcgg gtatgacatt
     4201 gtttctcctt aaaccacatc cggcagctta tcgagcagct tatccagagt gatgggataa
     4261 tcccgtaccc gaataccggt ggcgttatac accgcgttgg cgatagccgc gctcacgccg
     4321 cacaggccca gctcaccgac acctttggcc ttcatcgggg aggatatggg gtcggtatca
     4381 tccaggaaaa tcacctcctg ttttgggata tccgcatgga ccggcacctc ataccccgcc
     4441 atatcgtgat taacgaagta gcccaaacgg tcatccaccg ccagctcctc catcagtgcc
     4501 gcgcccatgc ccatagtcat tgcgccaatg acctggctgc gcgcagtttt cggattcagg
     4561 atgcgtcctg cagcacacac agcgagcata cgccggaccc gaacttctcc cgtcgcgcta
     4621 tgcacgccga cctccacaaa atgcccggca aaggtcgact gctggtactc tttgctcagt
     4681 gttccgaatt caatgctctc ttccgctgtc agtctgccgc ctgcggtggc ttcatgtagc
     4741 gtggcgcttc gggtaccgtt ggtaatcttg ccgtcggcaa actgcgactg ctcaggatca
     4801 aacccgactg ccgaggcaat catttcgcga agcttcatac aggcggcgta aacgccggag
     4861 gtggaggtat tcgcgcccca ttgtcctcca gaacccgcag aaaccgggaa actggaatcg
     4921 ccgaggtgaa ccgcaacctg ctccagcggt acgccaagca tttccgctgc cgtctgggcc
     4981 agaatggtgt agctgccggt gccaatgtcg gtcatgtccg tttctacggt aacggtgccg
     5041 ttttgttcga ggtgaacccg agcacccgat ttttccagca gattattgcg aaagcccgcc
     5101 gcaacaccgt ggccgactag ccactccccg tcgcgcacct gtccgggtgt ggcgttgcgc
     5161 tgcttccagc caaatttatc cgctccggtg cgcaagcact cgataagctg acggcgagag
     5221 aagcagcgcg tcgggtcggc ggggtcaacc tgagtgtcat tcaggatgcg aaactcgacg
     5281 ggatcgatgc ccgctttttc cgccagttcg tcgatcgcga tttcgagcgc catcagaccg
     5341 ggggcttcgc cgggcgcacg catggcgttc ccttccggca aatcaagcgt ggcgagccgc
     5401 aggccggtat gacgattcgc cccggcgtag agtaattcgc tttgctgtac cgccgtttcc
     5461 ggcgtgccgc cgggcaggtt tccagaccag ctttcatgtg agatagcggt gattttcccg
     5521 ctctggtcgg caccgatacg caagtgctga agggtggcgg ggcggtgcgt ggtgttattg
     5581 ggaatagagg ggcgggggag catcacttta accggacgtt tcaccgctcg ggcggcgagg
     5641 gccgccagca gcgcatcgct tctcaggaac agcttgccgc caaaccctcc gccgatatac
     5701 ggggagataa tacgcacatt ctccacggga actttcagcg tttttgccag atcggtgcgg
     5761 caccagtcaa tcatctgatt tgaggtccag agggtaagct tatttccatc ccagacggcc
     5821 atcgaggcat gcggctccat cgccatatgg ctctggtccg gggtcgtgta ggtagcatca
     5881 atcttcaccg cagcggaggt gaaagccccg tcaaagtcac cgacgttttt gtcgggcgtg
     5941 tcttccggcg gctgattgac ggcctgtttt tcgtccgcca gggagtaagc tcctttatta
     6001 cggcgatagt gtgcctgcac cagcgaggcc gccgctcgcg cctgttcgaa ggtctcggcc
     6061 actaccagcg caatggcctg atgatagtgc tcaatagtgg ggccgcctaa cagtctggcg
     6121 gtgtttttgt cgcctttgcc gagtgccccg gcgttactgg cggtaatgac agccagtacg
     6181 cccggcgctt tttgcgcggc gtccgtatca agggcggtga ggcgtccttt ggcaatggcg
     6241 gaaccgacga tatagccata ggcggcgttg ggggcttctt catgccattc gtaggcgtag
     6301 cgtgccgtgc cggtagtttt cagcggtccg tcgatgcggt catggggacg accgacaacc
     6361 ttcagctgat cgatcgggtt ttcccctgcg ggtttatcaa atttcatgcc tgtgccctcg
     6421 cttcagccag tacggaggca agcgttcgct tcgccaacag gagtttaaag gtattttcag
     6481 cggtgggatg ggcgctggcg aacagcgcgt catatacggc ctgcgccccc tgggatagct
     6541 gagcatccgc agcctcaatg cgccagggct tatgtgctac tccgcccagc gcgacgcgcc
     6601 cgctgccgtc aggctgaata atcgccgcga ccgataccag ggcaaaggcg taggaggcgc
     6661 gatcgcgcac cttacggtag atatgttttc cgccgagcgg tggaggtaac gtcaccgcaa
     6721 cgataagctc accgggaagc agggcggttt caatgtgcgg cgttttcccc ggtgggtgat
     6781 aaaaatcagc cagtgtgata ctgcgagtct ttccctccgg cgtgatggtt tccaccaccg
     6841 catccagcaa ccgcattgcg accgccatat cgctcggatg ggtggctatg caggcttcac
     6901 ttacgcctac caccgcgtgc tgacggctaa agccttcaag cgccgcgcag ccgctcccgg
     6961 gcaggcgctt attgcagggc tgattggtgt cgtaaaaata ggggcagcgc gtgcgctgga
     7021 gcagattacc tgcggtggtt gcctgattac gtaactgacc agatgcgcca gcgagcaggg
     7081 cgcgggagag taccgcgtaa tcacgacgca cgcgctcgtg agccgccagg tcggtgttcc
     7141 gtactagtgc gccgatgcgc agcccacccg cgtcggtcac ttcaatctta tcgagcccga
     7201 ggccgttcac atcgataagg tgggtgggcg tttcaatttc cagcttcatc aggtccagca
     7261 gattggtccc gcccgcgata aattttgcgc cgggtacgcg ctgagcgcta agtgccgcct
     7321 cggctggggt gttcactcgt tcataggtaa acgccttcat gattttatct cccccgcagc
     7381 atcttcaatg gcggcaagga tgttagcgta tgcaccacag cgacagatgt tgccgctcat
     7441 acgttcacgg atctcatcgg cagttgtttc tggagcggaa accaaatcga ccgtgacgtg
     7501 actgggaatg ccgtcctgaa tctcttttag cgccgctact gatgagcaaa tttgcccgga
     7561 ggtgcagtag ccgcactgga agccatcatg cttgataaag gccgcctgca tggggtgaag
     7621 attatctggc gaacccaggc cttcaatggt ggtgatctcg gccccctgat gcatgactgc
     7681 aagcgtcagg caggcattaa gcctgcgacc attgaccagc acggtacagg ctccgcactg
     7741 tccgtgatcg caacctttct tggtaccgat caaatgcaga ttttcacgca aagcgtccag
     7801 tagcgtggtt cgggtatcca cctcaagctg ctcggttttg ccgttcacct tcagtgtcag
     7861 gggcattatc tctggcgcgg gtgtagctgc cggaacgctt gccgccagcg tagaatgagg
     7921 ataaaccacg gcggtcgccg ctgttgcggc gctcacttta atcagatcgc gacgggtcaa
     7981 acttaaatcg tgcggctcgt gcttcccaac ccgattatct tcggggtatt cgccttggtt
     8041 gctcatacca ggcctccggt attctaaagg ggaaaataag agtgcgttgc gagagtgctg
     8101 atgctttttt aaatgttaag catagtcggc gacagcagga ggattattct taaaacgcga
     8161 atgcagctat gagcccggct aataaattca tgttcgccgg gatgttgatg atgatgggag
     8221 ctggtttatt gctttagttg tacgatgcaa aaaccaataa ggaaacctgt gattttcagc
     8281 tctgcatcac cctgcaaatc tctgtcactt ctaatataaa aatagggaga aatgatggag
     8341 cttatattca ttggcgatta ggaaaccatc ttgttataca aaacaataca gttctttaca
     8401 tttaccttgt tttatgaata ctcctgaaga ggtgtataac ataatggtac aagcagggta
     8461 gatatgaata tatttgaaca aactccaccg aaccgcagac gttatggtct tgctgcattc
     8521 attgggctga ttgctggcgt tgtttccgca ttcgtgaagt ggggctgaag ttccattgcc
     8581 gccacgtagc ccggtggata tgtttaatgc agcgtgtggc ccggaatcat taatcagggc
     8641 tgcaggccaa attgattgct cgcgtaattt tctcaatcca ccgtatattt ttcttcgaga
     8701 ctggttgggg ctgacagatc ccaatgcggc tgtttatacc tttgccgggc atgtctttaa
     8761 ctgggttggt gttacgcaca ttatcttttc gatagtgttt gctgtcggtt attgtgtggt
     8821 cgctgaagta tttccaaaaa ttaaactctg gcagggctta ctggcaggtg ctttagccca
     8881 actttttgtt catatgattt cattccctct catgggactg acgccacctc tgtttgatct
     8941 cccgtggtat gagaatgttt ctgaaatttt tggacattta gtctggttct ggtctattga
     9001 aattattcgc agagatttac gaaacagaat tactcatgag ccagaccctg agatcccttt
     9061 aggctcaaac agataatgca ttgaatgata aaaatggcgc aaatacagcg ccatttttat
     9121 aggttaaaaa cattgctttt tatattctga ttcagatagt cagtgagtat atcgcgctac
     9181 ttcaggatga tgtagatccg aagaacgcta cagaagagag gcattgttgc tggcaaatag
     9241 aagaagtatc gggttttgtt acccctgaaa aacgaagccc cgctattatc gctggcgggg
     9301 cagtgcaatt aattattgta tgtttataac ggtgtcagcc cgtatttagc ccttgcacga
     9361 ttgcaagcct cattgacgac tccgttctca ccagacatgg caaattctct gcatgtggac
     9421 gatcgatttt tatatatcgt gcagcaggca tttttgcccg gggttcctgc aagggcaata
     9481 catcgggggt ttttctgatt ggtaccgctc atgcatcggt gaaaagggga tatttgttca
     9541 gtgagcctgg cgggaatagt gccgccagca tcgtcggctt cggcccagta aaaagagacg
     9601 cggaaaaatg cacaacaggc accacacgtc atgcatggat tcagattgct cataattcac
     9661 cagtacagct aaaaatcgta aagagaacag cggtacatcg tatgtaagaa cgtaccgcgg
     9721 ttggctgggg aactttcgat agtgtgagta ttgaatgatt tccagccgtt accgatttta
     9781 cgtactaatt cgtgattaaa ccactcgtta gcaggctgcc tgataccttt ccgtgtctcc
     9841 tgaacactat ggtgtgcata tgagaaagcc catcgttaca ggtgatattg acgatcgctt
     9901 ccacatcatt taaacgattt ttggggctct gaggtggaat tcttccctcg accgatggat
     9961 aagtcttagt ccgccgaagg gggcttagcc ggacaggaat cgctaatctt aatgaatttg
    10021 tcgttataga ccagataatg attccccggc tgacgtaact caggctgatg aaccacgtct
    10081 cccggacgta gataccaggc atcgccttcc tcttcggtcg aatcacattc tggcttaatc
    10141 agtagcttaa accatgtgtt gccagtgtta cttaccgttc ctgtcacctt gtcgaaggac
    10201 catttaaact gaacctgacg tggtcgtact accagaatgg tatccatcac caccaccggc
    10261 tccgtgctga cctcgccgcc ggttgggcta cgtcttgtct ggttacgagt ggggacctcg
    10321 cgaaatgaga cccggtagta gcgctcgcgg ttatcccgtg gcccatgata gtaaaattta
    10381 aaatactcgc tctcaccagc ctgcaacgct agctggcggg gggcgaaaag cagttcacca
    10441 tccaccggtc gggtgcgcaa ttcactgctg cccgggctat caatagcact gatggcaata
    10501 cggtatatcc gtgcgctttt gttgttattc actacacgtt tgctgacaaa gtcagtctcg
    10561 gacggcagcg aaaatgtcag attgccgacc gaaatcgcct gtgtcaaagg tgtcgccagc
    10621 atacagaccg ctgtcagcag ggccttagtt aatgttacgc cacgtcgcct gaacatgaat
    10681 ttctcctgaa gcgctgactt cgccaaacca gccgttatca tctaccgtac gcagcgaaat
    10741 ggcgttatcc attggaatcg aaaatttgac tgtggtctta tccatctgcc ccacttcgcc
    10801 agagatatca gtccacggtg tggtcagcca cagcgcatcg gtcatatcac gccagctatc
    10861 atcgcacccg gcatcgtagg tttttgtagc tccgttgcgg gtaataaagg aaagcgtcgc
    10921 cgggaacggt actttcgcct taccgtcatc ggagctgaag acacaatagg agcgcccgcc
    10981 aatcacctgc gcgggtccgg tcaccttgat cagcacttcg tcggcagctg ttttaccgct
    11041 ggtggttacg atatagccga aatcgagtgc cgactcgccg ctgccaacat atccctcccg
    11101 cgacggtgtc tgagtatatt catcggagat aatactgatg ctgaaatcac ggggtttgat
    11161 aatcagcgtg ttggaggtag aaaattcata ccagccagac tccggtgatg tggtgttctg
    11221 aaagcgaaag ttgaatagat ctttggtgtt gatatcgctg atcccgaggt tcaccatctg
    11281 cttaaagaag ttgctcgaga agaaaacata gatcgagtct gcgctcttca tctcgttgaa
    11341 ggtgtagtaa taggcagtat tgctcaccgg tttccatgaa ctgccattca gactgaactg
    11401 catatcgtac gccccgacgg ccgaggctaa cgacgagttg gcgatcgccg ggaatatatg
    11461 gattgaggtg ttgctgagtc cgtttgtttg cagggtatag ttaaccatct tacaacttaa
    11521 tcctgaacgg ctgccgatgg tttgcgtccg gcagtcggcg ttcccttcgc ccagagtcgg
    11581 tactccgtcg ctgttgataa atacttccgc cagcgagtgg gtatttatca accgtagatt
    11641 tgctgctttc gtatgggtga cgttgcgaac ataccagttg ccggaggcct gatccttaca
    11701 gcgtgcgccg ctgctggcgt catagttcac agaggtctgg caggcattga tggtcattgt
    11761 aaagctgctg ccgacgggca tttgttgcag atactggtaa aacgcgtctg acaacatgcc
    11821 gtgcatccac ttcgcgccgc cgctggtcac cgtcgcgcca taaaatccac tggcgtcggt
    11881 ggtttgcggc aggataagac tggtggtcat attacaccca gcgtaccagt tgatgcagcg
    11941 caagccagtt aaaggagatg aaactggtga attttccagc cacatatcaa acttccagtt
    12001 ggtataaagg ccggtgttgt aaccgttatc aatgtagccg aggctttgct gatagatggt
    12061 tcctgaacca ttgtatttta aaccggtcca gcggttggca ccggtcaggc gcggatccag
    12121 cgccccgcca ggagtgacga aaaagttgtc gtctgagttg ttttcgacaa acacaaactc
    12181 gcgtgccggt gcatccgccc aggttgtttt gctcactgca gctctgagcg cggttgcagg
    12241 ccagattagc gcaaagatta tcatcgctat cagtaggtta actctcattc tcttctcctg
    12301 tctgcgtcac cgccgcccac gatgagaggc cgctgcagac cacatctccg acccagacgg
    12361 caccgcgcgc ctggttgagt tccagtgcca cttcgcaggt tttattgcca ctgtagcgaa
    12421 aatcgatagt ggggtatttc ttgtccacat ccatgacaaa ctcgccgttt tcatcggttc
    12481 gggttcggcc gatatggttg ttaatccgtg cgttagccag cagtgtgccg tcttccgcac
    12541 ggatacggcc ggagacggta accatctgct tcacctctgg ctcaatgaca gcgacattgc
    12601 ctggatagag agtcagatga cttttgcggc cgctgacgat atcgtaactg tcgagtgagt
    12661 ttttgctgtt ctgtaactcc acctcatatc ttccataggg agagagcggg agatagttac
    12721 gcttgccgtt aagcgggaaa atccgcccgt tgattttggc gctgatctga ccgtcgtcct
    12781 ccagcccggt gttgaatatc accccagcgt tgccatcagt ccgcccgctg gcagcaatgt
    12841 ttttaccctg ccagccgacg ctgccattgg cggttaaatt ggtattgacg tagccgtccg
    12901 ccgcgctatt gacgttcagc gttccgctgg cgtaacgagc gtcgaactgt gcatacgccc
    12961 caccgctgag ggttttgtca tcaccggtat cgccggagat ggctcgtgac agattggcac
    13021 caacagtgcg aatggtccct tcatcaaact gcttgcgtgc tgacaggttt gccatggtgt
    13081 agccgttttg atgagtcatc cctgcgctaa accagttgcc cagtggtagc gagagatcga
    13141 gagcgatata tttccctgta ttggcgttgc tgtcgccgtt gttatagcgc tgaataccgg
    13201 cccgcaggcc aagcgaacca aagggaccgc tgtagacatt ttgatagtaa tctgccgtgt
    13261 aataatggct gttgtaacgg cggtcatcat tgtagctgat gctgaatgtg cccagcttcg
    13321 accacagtga gttcaggttg agtgtgccgc cgattgcacg gttgtcggca tcgctacgtc
    13381 gcaattgatt gccaatgcgg gttttttcct ggttaaccca cagcgaacta aagcctcccg
    13441 gtagagtggc gctgatgctg gcgatgttgc tccatgagct gtcactggcc agcatatttt
    13501 gcaggttaac gttgatcgct cccccaagcg gcagcgtcag acgggtttca cccaccgcct
    13561 gattatcgta tccatatccc gttgccgccc agctaagcgt actcaatgag ccggaggtcg
    13621 aggcacctgc tagccaactc tctttagctg gtcgcgtctt tttcccgttt tccgaccagc
    13681 gatccatatg aaagctaccg ccccatacct gccacgccag tggtgcaccg acgccgcgcc
    13741 cccggctaaa cagcttattg acccgctggg ggcgtttgct gatcacgcga ccgttaacga
    13801 tcacctcaac ttccacatcg taaatcccgt acggtagacc ccgggtatcc acttcatgat
    13861 tgcccatagt gaagttctga acgcttagta gccgcccatc acgggtgaga tgtacttcgc
    13921 ccgccgccgg taaaaaggcg atcactggcg tggctgactg gctgctgtcg aagatggtgg
    13981 agctggcctg gtttccccag gaaaggccgt aaatcttccc tgctgaaatg gcggtcatcg
    14041 gccctaagga ctgcaagttc caggtgtcga gcattccacc ggcaaatcgg tgaccggcaa
    14101 aatcgcgttc atacatcgct ttatataatt cactgtcctg ttgaccgcta ccgatcccgt
    14161 acagcgagcc gtcgagcacc acatgatgtt cgcgcagtgc agtaacgtta ttcagcgaca
    14221 gatagctgga tgtgttgctc ccgccgttac gcaactggtt gttatagatg ccgaagttat
    14281 agctcagatt actgctgagg gtgttaacac tggactgccc gatgtcttcg ctacgtgagc
    14341 gtagtacagt gcccagcgct tcgcgcttga ccaccagctg cagcagcagt tggcgcaagc
    14401 tgagatccag ctgcgcgttg tcagtcaggg ggatggtaag ggcctcattg aacggggcgt
    14461 tcgccagagc catcagctgc tgtcgagttt gttcgctgac actggcgtta tcttcactct
    14521 cttccagctg tattttccgg atgcgtagct gtccatgatc caaccagata aaagcgctgc
    14581 cgattcgctg atcgtcctgg cgaccctggc taccggcgag atgaatataa agcgggacgc
    14641 tcatgccgtc ctggagcgcc tgactgaagg cctgcggaat aatcacccca cctatttgct
    14701 gcgcacttat gtcagcagcg ctggcatcgg gctgaacgaa caaaaagacc atgccgaagg
    14761 caaactgggc tttcagtcct ggggagaacc gtcgtaaagg cattgtcgtt atccatctgc
    14821 acagctatct atttcacggg aatgaactta tcaccctgcc aaagtgcaac ccgtcctttg
    14881 ttatccgccg tgtccacgcg ggtaaaacga cgcgacttgc ccggcatcag gtagtaattc
    14941 tctttacact ccttaccatt ggcggctttc aggcaggggc cgtaagcgag gatccgcagc
    15001 gtcgcatttc ctgtatttgt cagggagccg ttggcgtact gaaagtggta gtttgcctgg
    15061 cggggtgcga cgaccagaat ggtgccgatg cgggcggaag cagtggccac agcgctgcgg
    15121 ttggcattat cgcgctgcgc atcactgagg gcctgatcaa accagacaat gcggtagtag
    15181 cgctcttttt catctgctgg tcccttatag aagaagcgga tcacttcgct ggcttgggcg
    15241 ggtagcagca agctggcggg agtgagtagc aactcatccg gcttgtccat tgcgataacc
    15301 tgcctgtcgt caagcggtga agagagccgt tcgagacgga tattgatgag gcgaccactg
    15361 tcggtactgt ttttgatcgt tttgctcagc gtgctgctgt cactgttcat aaacgatgat
    15421 atatcgccga catccagcgc ctgggccgga gatattccgg ataacagcag agcgagaagc
    15481 agaaggtgct ttttcataac aattccgtcc aggaataaag cagggggggg ctacccctgc
    15541 tggtacatca gagagattaa ctggtccagg tcgcgtcgaa ctgtacgcta acgtcgccgc
    15601 tccagatgcc ttccggtaga gtgctgtaat cggttactgc ggtggtacca ttggtggtgc
    15661 cgctgatgat ggagaaagtg aaaccatcct gtgcggtggt acgattgctg gcattgtaac
    15721 cgttagccag cgggctaagg gtgccgccca gtacgccgtt ggcggtatcg atcatcacgg
    15781 tatcgccagt tttttcgact gccgcgccgt tataatccac gcccacattc agtgtagaac
    15841 ctgaggtatc caactgggtt aaggtgttgg tgataagacg tgaggtcagt ttaaaggcgg
    15901 tagccgttga gtcaccctcg atagccacgt caaatagacc tttctgtgag ttaaaacctt
    15961 taatgccttc ggcatactgg aacgccaggc taccgagtgg cgtcacaacc agcttactgg
    16021 tggtgtcttt tttggcagta cacctcacag gtcgctacag cctgagctgt tacgtcagca
    16081 gcctgcgcca cacccatgcc ggtaaacacc gttaccagag ctattgccag aacctttttt
    16141 ttcattgcat ttcttcccga gttgaattga ggacgtgatg tcccagtgag tcatttttta
    16201 aaactaactt gcctggagtt tactgaacca acttatatat ttttgagtac agcttggcct
    16261 ctgcattacg ccgatgggta tacactgtct ttacactaca attttcaatt ctggcaatcg
    16321 atttaggcag cattccctga gccgtcatgc ggataatttc catttctcgg tcagtgattt
    16381 tatctttctt aatatctttt ctcaggaacc tgccattaat cacataggcc agttcttttc
    16441 taatatcacg actcagtcca gcgtatacca cgcccctaat attgctgttg taataccaat
    16501 aatttgctaa ggcttctgac tttctggccg caatcaatac gacctgtttc ccctttaccg
    16561 cggataacca ctcattatca cggtttataa attcagttaa agaatccgta ttgagattga
    16621 taaacacgaa atcttttctg attttctcca gcgatagata aattaatctg tcgatatcca
    16681 caatcagttc agatagtcct ttatagaagt aggcgtcatg gggagaccag atgtatttat
    16741 cagaacggtt ttgacattcc atatggtttt taacctcata gtccctgctg taatcatttt
    16801 gccatgtcac tactttccaa acctgtaatt tttacaggta cattcagttt ctttagtaaa
    16861 tcggaagtaa ataagatacg tagcacatat caatataggt taaacgagat taagttttaa
    16921 ttatttcgat taggtttaaa tttatgtttt tttcctgatg agtgtcaata tgtgaacaga
    16981 aaaggaattc aatggttaag tcaatattta gaaaaactga aaatattgct tttttgataa
    17041 gaagaaaaag acattcagtt tcataaggtt attggcaata taacgttcaa ttcttacttg
    17101 cattttatat tagccaggaa ctttgccctg aggtgttagg aatagtctta atgtgttgtg
    17161 taatggacta gtaaaaaaaa caagtttaaa tgataagaaa tagaatataa aactatcatg
    17221 agtcataatt aaagatgatt tgccaatcag taaggtgttt taatggatta acatgccgca
    17281 caaaatggtt atgggtgttc atcccaataa gtgatgaaaa attaaacagg tataagtacg
    17341 tcatctgtcc ggtaagcccc ctaagagcca aattaagtga agttaaacga agactgccag
    17401 ttgtcacagg aacgatggcc tctttgccag ggcactgcat tcagtcatct gtgcgcgtta
    17461 ctctaactgg cactcgaacg tcggcgatgg ggttaccggt ggatcaggcc gttcctgtgg
    17521 cgtaaacgtt tttatgtcag taccaacaga tttaccacat ttatcatctt aacagtttga
    17581 gtgtaaatgc aggctgcgtc gcaaagggag gaaactgagt ggcgtgttca gtctaaaaga
    17641 gggttgatgc cagcatacag ggcgaagctg gtcaacaagt caacattaat ataatggaat
    17701 attcggagag aaacgtcagg cagttgacct taaatgaaca ggaagataca tccccagaaa
    17761 aattaatgct ggtgtaataa atacaccaac attaatccgg tctgattatt aatcagacaa
    17821 atatcgttaa ttcgtcgaat tttaaatcaa cgttttttct tacgaccctg aacggcctta
    17881 aaacgtggat tagatttaca aatcacatac agccgtcctt ttcgcttcac aatctgacag
    17941 tctggatggc gttcttttgc ggtacgcaga gagttaagga ctttcatcat gccccctttt
    18001 tcgtgctaac aaaacgacca aaacgttggg tgaaccgtgc aacatttcct tctgatgcca
    18061 ctgttctcag cttccctgta tagaacgggt gcgatttcga agagacatca attgtcacgt
    18121 atggatacgt tacgccatcc agctcaatct cacggtctgt tttgatagtc gagccgattt
    18181 taaagtactc atcaacactg gtgtcgtgga acaccacagt acgatactca ggatggatat
    18241 tgggcttcat cattttacct gttatgttat aacataacca taaagataca tgctcatccg
    18301 gatcatttca acacccaatc cctgtgccca tttttggtat gtggaatttc cccaacgggg
    18361 aagtctggca ttgttagcag ccgttggcga cgctcccata aacgcctgga tgagtgattg
    18421 atgatggttt cgctgagagt atggattttt cgacaatgga ggttatctcc actttactgg
    18481 ccgatgattt atcgcagtat gattgattac ctgacagcgt gtattcctct cccacggata
    18541 taataattac ttacaaaaag ggagaggatg catattttaa atatcactga agtgaacagt
    18601 ttattgccgt tattaataga aatggagaaa taaataggcg tattctacaa ttgcgacaaa
    18661 aacaacgata ttaatcagtt tatgactgat ttgttgtact ttattctctt tcattggtac
    18721 ttcctcgctt taaaaaagag cgcacttcgt aagtgccctt atataaataa cgagtttggt
    18781 caaccaattt tttgacatgt atcacaaatt tgaatggatg tattacatcg gctatctttt
    18841 attgcaccaa cgtcattgat atatgtcgcc tgaagtcagt tccgggaatg agtctgatct
    18901 caagactggc ccagtccggg cgttgattgg cgctgaggag catatcgcat ctcatcataa
    18961 tgtcgtatct cctggggtgt tatacaagat atcgttgttg gtgacctggg agaggaattg
    19021 agttctatta aaccgtcaac tatgccggat acatactgga ttacactgca ggcacgcctt
    19081 acgagagaac gtgccgcagt gacgggttaa ttatctgaaa gaatttgcga ggctgtatcg
    19141 gttactcatt ggtttgatag ttttactctc gggagagtaa tagatattta attcattaac
    19201 ggaaaccagt cagttccttt cgatgcctga atttgatccc atagtttatc cggaatggtt
    19261 aattcatgaa caagttgtgt tatcgttcat gagaagcata acgtaaaggg aaaagctcga
    19321 ttagacggca ggatttgtca ggggttatga acgaaattca taaatctgtt tgagtgttgc
    19381 gatgggtagt gcaagttcga tatctccgca atttacagtc tgatgaatga aaatgaatat
    19441 ccataaaaaa tatattggtt tatcctggcg tatataccta cttcgatgta ttgccaatcg
    19501 tggtaaatta aagacaggtc attattattc actctgaata atgaattatt cactgtccgc
    19561 agagtaagaa atataactta ggtatctatt taatgacttg cacaaaaagc taaattttta
    19621 cccataaata aaaatataat cccgcgccca accacctgat gagtggctac aggcactgga
    19681 tatattaggt ggcggtgtac tttcttacat aaaggtattt ccttttctgc ggaaaaggaa
    19741 accgggaaat ctccggtttt tctgacaagc agacgccatt atttgtgtct gcctatgttc
    19801 gttaattcgt tcatcaggaa attatctcaa tgtcacgtta taaaacaggt cataaacaac
    19861 cactatttcg ttattcagtt ctggcccgct gcgtggcgtg ggcaaatatc tctgttcagg
    19921 ttctttttcc actcgctgtc acctttaccc cagtaatggc ggcacatgcg cagcatgcgg
    19981 ttcagccacg gttgagcatg gaaaatacca cggtaactgc tgataataat gtggagaaaa
    20041 atgtcgcgtc gtttgccgca aatgccggga catttttaag cagtcagcca gatagcgatg
    20101 cgacacgtaa ttttattacc ggaatggcca ccgctaaagc taaccaggaa atacaggagt
    20161 ggctcgggaa atatggtaca gcgcgcgtca aactgaatgt cgataaagat ttctcgctga
    20221 aggattcttc gctggaaatg ctttatccga tttatgatac gccgacaaat atgttgttca
    20281 ctcagggggc aatacatcgt acagacgatc gtactcagtc aaatattggt tttggctggc
    20341 gtcatttttc aggaaatgac tggatggcgg gggtgaatac ttttatcgat catgatttat
    20401 cccgtagtca tacccgcatt ggtgttggtg cggaatactg gcgcgattat ctgaaactga
    20461 gcgccaatgg ttatattcgg gcttctggct ggaaaaaatc gccggatatt gaggattatc
    20521 aggaacgccc ggcgaatggt tgggatatcc gcgcagaggg ctatttacct gcctggccgc
    20581 agcttggcgc aagcctgatg tatgaacagt attatggcga tgaagtcggg ctgtttggta
    20641 aagataagcg ccagaaagac ccgcatgcta tttctgccga ggtgacctat acgccagtgc
    20701 ctcttctgac actgagcgcc gggcataagc agggcaagag cggtgagaat gacactcgct
    20761 ttggcctgga agttaattat cggattggcg aacctctgga gaaacaactc gatacagaca
    20821 gcattcgcga gcgtcgaatg ctggcaggca gccgctatga cctggttgag cgtaataaca
    20881 acatcgttct tgagtaccgc aaatctgaag tgatccgtat tgctctgcct gagcgtattg
    20941 aaggtaaggg cggtcagaca ctttccctgg ggcttgtggt cagcaaagca actcacggac
    21001 tgaaaaatgt gcagtgggaa gcgccgtcat tactggctga aggtggcaaa attaccggtc
    21061 agggtagtca gtggcaagta acgctcccgg cttatcgtcc aggcaaagac aattattatg
    21121 cgatttctgc ggttgcctac gataacaaag gcaatgcctc aaaacgcgtg cagacagagg
    21181 tggtcattac cggagcaggt atgagtgccg atcgcacggc gttaacgctt gacggtcaga
    21241 gccgtattca aatgcttgct aacggtaatg agcaaagacc gctggtgctg tctctgcgcg
    21301 acgccgaggg ccagccagtc acgggcatga aagatcagat caagactgaa ctaactttca
    21361 aaccggctgg aaatattgtg actcgtaccc tgaaggccac taaatcacag gcaaagccaa
    21421 cactgggtga gttcaccgaa actgaagcag gggtgtatca gtctgtcttt actaccggaa
    21481 cgcagtcagg tgaggcaacg attactgtta gtgttgatgg catgagcaaa accgtcactg
    21541 cagaactgcg ggccacgatg atggatgtgg caaactccac cctgagcgct aacgagccgt
    21601 caggtgacgt ggttgctgat ggtcagcaag cctacacgct gacgctgact gcggtggata
    21661 ctgatggtaa cccggtgacg ggagaggcca gccgcttgcg atttgttccg caagacacta
    21721 atggtgtcac cattggtaca atttcggaga taaaaccagg cgtttacagc gctacggttt
    21781 cttcgacccg tgccggaaac gttgttgtgc gtgctttcag cgagcagtat cagctgggca
    21841 cattacaaca aacgctgaag tttgttgccg ggccgcttga tgcagcacat tcgtccatca
    21901 ccctgaatcc tgataaaccg gtggttgggg gtacagttac ggcaatctgg acggtaaaag
    21961 atgcctatga caaccctgtg accagcctca cgccggaagc gccgtcatta gcgggtgccg
    22021 ctgctgtagg ttctacggca tcgggctgga caaataatgg tgatgggacg tggactgcgc
    22081 agattactct cggctctacg gcgggtgaat tagaagttat gccgaagcta aatggacagg
    22141 atgcggcagc aaatgcggca aaagtaaccg tggtggctga tgcgttatct tcaaaccagt
    22201 cgaaagtctc tgtcgcagaa gatcacgtaa aagccggcga aagtacaacc gtgacgctta
    22261 ttgcaaaaga tgcacatggc aacgctatca gtggtctttc gttgtcggca agtttgacgg
    22321 ggaccgcctc tgaaggggcg accgtttcca gttggaccga aaaaggtgac ggttcctatg
    22381 ttgctacgtt aactacaggc ggaaagacgg gcgagcttcg tgtcatgccg ctattcaacg
    22441 gccagccagc agccaccgaa gccgcgcagt tgacggtcat cgccggagag atgtcatcag
    22501 cgaactctac gcttgttgcg gacaataagg ctccgaccgt caaaacgacg acggaactca
    22561 ccttcaccgt gaaggatgcg tacgggaacc cggtcaccgg gatgaagcca gatgcaccag
    22621 tgtttagcgg tgccgccagc acggggacag agcgaccttc aacaggagac tggacagaaa
    22681 caagtaatgg ggtctacgtg gcgaccttga ctctgggatc tgccgcgggc cagttgtctg
    22741 tgatgccgcg agtgaacggc caaaatgccg ttgctcagcc actggtgctg aatgttgctg
    22801 gtgacgcatc taaggctgag attcgtgata tgacggtgaa ggttgataac cagctggcta
    22861 atggacaatc gactaaccag gtaaccctga ccgttgtgga cacctatggt aacccgttgc
    22921 agggacaaaa tgtgacgctg actctgccga aaggtgtgac cagcaagacg gggaatacgg
    22981 taacaaccga tgcggcaggt aaagccgaca ttgagctgat gtcaacggtt gccggggaac
    23041 acagcatcac ggcctcagtg aataatgctc agaagacggt tacggtgaaa ttcaaggcgg
    23101 atttcagtac cggtcaggcg agtctggagg ttgatagcgc cgcgccaaaa gtggcaaacg
    23161 gcaaagatgc ctttacgctg acggcgaccg ttgaggataa aaatggtaac cctgttccag
    23221 ggagcctggt gacctttaat ctgccccggg gtgtcaagcc gcttacaggc gataatgtct
    23281 gggtgaaagc caacggtgag gggaaagcag agttgcaggt ggtttcagtg actgccggaa
    23341 cgtatgagat cacggcatcg gcggggaata gccagccttc ggatacgcag actataacgt
    23401 ttgtagccga taaggctacc gcaaccgtct ccggtattga ggtgatgggc aactatgcgc
    23461 tggcggacgg caaagccaaa caaacgtata aagttacggt gactgatgcc aataacaatt
    23521 tggtgaaaga tagcgaagtg acgctgactg ccagcccggc ttcgttaaac ctggaaccga
    23581 atggcactgc gacaacgaat gagcaagggc aggctatttt caccgctacc actactgttg
    23641 cggcgacata cacactcaag gcgcaagtga gtcagaccaa cggtcaggta tcaacgaaaa
    23701 ctgccgaatc taaattcgtt gcggatgata aaaacgcggt actcaccgca tcatctgata
    23761 tgcaatctct ggtggcggat gggaaatcga ctgcgaagct ggaggtgaca ctgatgtcgg
    23821 caaacaaccc cgttggcggg aatatgtggg tcgacattca gacgccggaa ggggtgacgg
    23881 aaaaggatta tcagttcctg ccgtcgaaaa atgaccattt cgtgagcgga aaaatcacgc
    23941 gtaaatttag taccagcaag cctggtgtct atacgttcac atttaacgcc ctgacctatg
    24001 gcgggtacga aatgaagcca gtgacggtga ccattaccgc ggtggatgcc gatacggcaa
    24061 aggacgagga ggcgatgaaa taattttaag aatatgaaag taacattcta ataaacaata
    24121 atggcgttga catcttcaac gccattattt attgaatttc ct
//
LOCUS       DAZFYC010000073        23016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFYC010000073 DAZFYC010000000
VERSION     DAZFYC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.073"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            604..672
                     /locus_tag="ACHKW5_003173"
                     /pseudo
     CDS             604..672
                     /locus_tag="ACHKW5_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            731..1201
                     /locus_tag="ACHKW5_003174"
     CDS             731..1201
                     /locus_tag="ACHKW5_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512517.1"
                     /translation="MTKSWSVPFPELETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            1345..1656
                     /locus_tag="ACHKW5_003175"
     CDS             1345..1656
                     /locus_tag="ACHKW5_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO7512518.1"
                     /translation="MKLRVWHIPQVPMKPFIVEVASVEEGVRLMDALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEMGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            1729..1893
                     /locus_tag="ACHKW5_003176"
     CDS             1729..1893
                     /locus_tag="ACHKW5_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO7512519.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            1862..2005
                     /gene="kil"
                     /locus_tag="ACHKW5_003177"
     CDS             1862..2005
                     /gene="kil"
                     /locus_tag="ACHKW5_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO7512520.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILMLKLRSSK
                     SIH"
     gene            2081..2377
                     /locus_tag="ACHKW5_003178"
     CDS             2081..2377
                     /locus_tag="ACHKW5_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7512521.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            2383..3168
                     /gene="bet"
                     /locus_tag="ACHKW5_003179"
     CDS             2383..3168
                     /gene="bet"
                     /locus_tag="ACHKW5_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7512522.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            3165..3845
                     /locus_tag="ACHKW5_003180"
     CDS             3165..3845
                     /locus_tag="ACHKW5_003180"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7512523.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3842..4024
                     /locus_tag="ACHKW5_003181"
     CDS             3842..4024
                     /locus_tag="ACHKW5_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO7512524.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            3997..4188
                     /locus_tag="ACHKW5_003182"
     CDS             3997..4188
                     /locus_tag="ACHKW5_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7512525.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            4265..4480
                     /locus_tag="ACHKW5_003183"
     CDS             4265..4480
                     /locus_tag="ACHKW5_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7512526.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            4579..4800
                     /locus_tag="ACHKW5_003184"
     CDS             4579..4800
                     /locus_tag="ACHKW5_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7512527.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            4797..5204
                     /locus_tag="ACHKW5_003185"
     CDS             4797..5204
                     /locus_tag="ACHKW5_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7512528.1"
                     /translation="MSEINSQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            5206..5961
                     /locus_tag="ACHKW5_003186"
     CDS             5206..5961
                     /locus_tag="ACHKW5_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512529.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHMNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAL"
     gene            5972..6097
                     /locus_tag="ACHKW5_003187"
                     /pseudo
     CDS             5972..6097
                     /locus_tag="ACHKW5_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            6095..6637
                     /locus_tag="ACHKW5_003188"
     CDS             6095..6637
                     /locus_tag="ACHKW5_003188"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO7512530.1"
                     /translation="MRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWAD
                     MQFLLWDAQRRAGISDAEITAAMENKLKINMERQWPEPKDGEPRLHIKEPGNSPVTPD
                     GWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGKPTWDDGDGED
                     LYLKEVTHWMQLPEPPQEVK"
     gene            6725..6967
                     /locus_tag="ACHKW5_003189"
     CDS             6725..6967
                     /locus_tag="ACHKW5_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7512531.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            6971..7105
                     /locus_tag="ACHKW5_003190"
     CDS             6971..7105
                     /locus_tag="ACHKW5_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512532.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWFHCRG
                     "
     gene            7124..7378
                     /gene="xisR"
                     /locus_tag="ACHKW5_003191"
     CDS             7124..7378
                     /gene="xisR"
                     /locus_tag="ACHKW5_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7512533.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            7412..8698
                     /locus_tag="ACHKW5_003192"
     CDS             7412..8698
                     /locus_tag="ACHKW5_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7512534.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            8719..9420
                     /gene="mlrA"
                     /locus_tag="ACHKW5_003193"
     CDS             8719..9420
                     /gene="mlrA"
                     /locus_tag="ACHKW5_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO7512535.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDILPLGI"
     gene            9480..9587
                     /gene="yohO"
                     /locus_tag="ACHKW5_003194"
     CDS             9480..9587
                     /gene="yohO"
                     /locus_tag="ACHKW5_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO7512536.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(9568..10299)
                     /gene="yehW"
                     /locus_tag="ACHKW5_003195"
     CDS             complement(9568..10299)
                     /gene="yehW"
                     /locus_tag="ACHKW5_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO7512537.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(10304..11230)
                     /gene="yehX"
                     /locus_tag="ACHKW5_003196"
     CDS             complement(10304..11230)
                     /gene="yehX"
                     /locus_tag="ACHKW5_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO7512538.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(11223..12380)
                     /gene="yehY"
                     /locus_tag="ACHKW5_003197"
     CDS             complement(11223..12380)
                     /gene="yehY"
                     /locus_tag="ACHKW5_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO7512539.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(12387..13304)
                     /gene="osmF"
                     /locus_tag="ACHKW5_003198"
     CDS             complement(12387..13304)
                     /gene="osmF"
                     /locus_tag="ACHKW5_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO7512540.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(13515..15812)
                     /gene="bglX"
                     /locus_tag="ACHKW5_003199"
     CDS             complement(13515..15812)
                     /gene="bglX"
                     /locus_tag="ACHKW5_003199"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO7512541.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            16008..17723
                     /gene="dld"
                     /locus_tag="ACHKW5_003200"
     CDS             16008..17723
                     /gene="dld"
                     /locus_tag="ACHKW5_003200"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7512542.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(17761..18693)
                     /gene="pbpG"
                     /locus_tag="ACHKW5_003201"
     CDS             complement(17761..18693)
                     /gene="pbpG"
                     /locus_tag="ACHKW5_003201"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO7512543.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATAASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(18867..19454)
                     /gene="yohC"
                     /locus_tag="ACHKW5_003202"
     CDS             complement(18867..19454)
                     /gene="yohC"
                     /locus_tag="ACHKW5_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO7512544.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            19624..20202
                     /gene="yohD"
                     /locus_tag="ACHKW5_003203"
     CDS             19624..20202
                     /gene="yohD"
                     /locus_tag="ACHKW5_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7512545.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVAFGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(20332..21093)
                     /gene="yohF"
                     /locus_tag="ACHKW5_003204"
     CDS             complement(20332..21093)
                     /gene="yohF"
                     /locus_tag="ACHKW5_003204"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7512546.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(21146..22582)
                     /gene="mdtQ"
                     /locus_tag="ACHKW5_003205"
     CDS             complement(21146..22582)
                     /gene="mdtQ"
                     /locus_tag="ACHKW5_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO7512547.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAGSNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            22806..22889
                     /gene="yohP"
                     /locus_tag="ACHKW5_003206"
     CDS             22806..22889
                     /gene="yohP"
                     /locus_tag="ACHKW5_003206"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO7512548.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         5951 a         5909 c         5805 g         5351 t
ORIGIN      
        1 gatgaattat ctctagcggt gttgacacaa ataccactgg cggtgatact aaacacatca
       61 gcaggacgca ctactcacca gggcggtgaa tatacaacga ttcgaatatg aatctacggc
      121 gctgacaaag cgcaataacc aaagtgaact ttggggtgtg gtgaagggtt catggacggg
      181 aatatgtcgc acgtaaagcg gcgaggcctg cgggactatt gccgaattga agtaggtcga
      241 aatgggtctc ccacctacca caccaccaaa gttcatcagg aggtctatat gacacgcaga
      301 actcagttca aaggcaattc acgttctcgt cgtcgtgagc gtttaaaggt aaaggcatta
      361 gctaacggcg tactggcccg cgaagaagca ataagttcag aagtattaca ccgccctact
      421 ctaagcagag cgcagattca ggctaaaggt actcacgaaa cgcctgagcg tatagaagac
      481 gctaagccaa ttaagttcat ggcacaggac gtgatctggc aacagaaaga atacagacgc
      541 aatctggagc gagcggccat tgtgtacgcg aatgagtttg gacataagca accagaaact
      601 ggtgtatgtc ttccaaacgt agccatttac gcggcaggct accggaaatc caaacaactg
      661 acggcgaggt gacttgtgtt ggtcgccaga aaatgaaatt aggcagcaaa ccacttattt
      721 gaggtgagat atgacaaaat catggagcgt accttttcct gaattagaaa ctgaacatga
      781 tggaatgcct gttttctgga gattccaggc gacagttgaa gaagatggga taaaaatatt
      841 cgcacttcaa tatatagctt ttcatcagac agagcattat gcatggttgg ttcctgcgca
      901 ttggattgtt aattttaaac cagcaccaaa tcagtggtta caggaatgga aacaaaggag
      961 aaatagatat gcaattaaga aagtagcaaa aaatgcagaa agatcttttg cattcccaac
     1021 gaagaaactt gctattgaaa gtttattgcg ccggaagaaa taccatttaa tgagaataaa
     1081 acaagatttg gctgttgtat caactcttgt tgatgggatg aaaaatattg atacatcaac
     1141 accagatatt gaatataact ttggacataa ccaagaaaca gaaaattggg tattttatta
     1201 gtacgaataa gcactgtgta ttcattccaa cgagtgaata cacggagcaa tgtcgctcgt
     1261 aactaaacag gagccgactt gttctgatta ttggaaatct tctttgccct ccagtgtgag
     1321 ggcaattttt ttgacggagg atatatgaaa ttacgtgtct ggcatatccc gcaagtacct
     1381 atgaagccgt tcattgtaga agtggcaagt gttgaagagg gtgttcgtct gatggacgca
     1441 ctggctgatt atgacgcctt tcagtatgac aacaacatca agcctgatta ctgcaatgct
     1501 aacggccttg agatgtggga tgagagcctt accgatgaag atttatcaga gatggggctt
     1561 actgatcgct gggtggattg gtacagcgaa tgccaatgtt acgacgaccc acgtaaatat
     1621 ctcgaaagcc tgaaagaaga aacatcagcc gcctaagcgc ggctttaccg cataccaata
     1681 acgcttcact cgaggcgttt ttcgttatgt ataaataagg agcacaccat gcaatatgcc
     1741 attgcagggt ggcctgttgc tggctgccct tccgaatctt tacttgaacg aatcacccgt
     1801 aaattacgtg acggatggaa acgccttatc gacatactta atcagccagg agtcccaaaa
     1861 aatggatcaa acacttatgg ctatccagac taaattcact atcgccactt ttattggcga
     1921 tgaaaagatg tttcgtgaag ccgtcgacgc ttataaaaaa tggatattaa tgctgaaact
     1981 gagatcaagc aaaagcattc actaaccccc tttcctgttt ttcctaatca gcccggcatt
     2041 tcgcgggcga tattttcaca gctatttcag gagttcagcc atgaacgctt attacattca
     2101 ggatcgtctt gaggctcaga gctgggcgcg tcactaccag cagatcgccc gtgaagagaa
     2161 agaggcagaa ctggcagacg atatggaaaa aggcctgccc cagcacctgt ttgaatcgct
     2221 atgcatcgat catttgcaac gccacggggc cagcaaaaaa gccattaccc gtgcgtttga
     2281 tgacgatgtt gagtttcagg agcgcatggc agaacacatc cggtacatgg ttgaaaccat
     2341 tgctcaccat caggttgata ttgattcaga ggtataaaac ggatgagtac agcactcgca
     2401 acgctggctg ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc acaggaactg
     2461 atcaccactc ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca gttcatcgca
     2521 ttgctgatcg tcgccaacca gtacggcctt aatccgtgga cgaaagaaat ttacgccttc
     2581 cctgataagc agaacggcat tgttccggtg gtgggcgttg atggctggtc ccgcatcatc
     2641 aatgaaaacc agcagtttga tggcatggac tttgagcagg acaatgaatc ctgtacatgc
     2701 cggatttacc gcaaggaccg taatcatccg atctgcgtta ccgaatggat ggatgaatgc
     2761 cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca ccggaccgtg gcagtcgcat
     2821 cccaaacgga tgttacggca taaagccatg attcagtgtg cccgtctggc cttcggattt
     2881 gctggtatct atgacaagga tgaagccgag cgcattgtcg aaaatactgc atacactgca
     2941 gaacgtcagc cagaacgcga catcactccg gttaacgatg aaaccatgca ggagattaac
     3001 actctgctga tcgccctgga taaaacatgg gatgacgact tattgccgct ctgttcccag
     3061 atatttcgcc gcgacattcg cgcatcgtca gaactgacac aggccgaagc agtgaaagct
     3121 cttggattcc tgaaacagaa agccactgag cagaaggtgg cagcatgaca ccggacatta
     3181 tcctgcagcg taccgggatc gacgtgagag ctgtcgaaca gggggatgat gcgtggcaca
     3241 aattacggct cggtgtcatc acagcgtcag aagttcataa tgtaatagca aaaccccgct
     3301 ccggaaaaaa gtggcctgac atgaaaatgt cctacttcca caccctgctt gctgaggttt
     3361 gcaccggtgt ggctccggaa gttaacgcta aagcgctggc ctggggaaaa cagtacgaaa
     3421 acgacgccag agccctgttt gagtttactt ccggcgtgaa tgttactgaa tccccgatca
     3481 tctatcgcga cgaaagtatg cgcaccgcct gctctccgga tggtttatgc agtgacggca
     3541 acggccttga actgaaatgc ccgtttacct cccgggattt catgaaattc cggctcggtg
     3601 gtttcgaggc cataaaatcg gcttacatgg cccaggtgca gtacagcatg tgggtgacac
     3661 gaaaagatgc ctggtacttt gccaactatg acccgcgtat gaagcgtgaa ggactgcatt
     3721 atgtcgtgat tgagcggaat gaaaagtaca tggcgagttt tgacgagatg gtgccggagt
     3781 tcatcgaaaa aatggacgag gcactggctg aaattggttt tgtatttggg gagcaatggc
     3841 gatgacgcat cctcacgata atatccgggt aggcgcgatc actttcgtct actccgttac
     3901 aaagcgaggc tgggtatttc ccggcctttc tgttatcaga aatccactga aagcacagcg
     3961 gctggctgag aagataaata ataaacggga ggcggtatgc acaaagcatc tcctgttgag
     4021 ttaagaacga gtattgagat ggcacatagc cttgctcaaa ttggagtcag gtttgtgcca
     4081 ataccagtag aaacagacga agaatttcat acgttagcca catccctttc acaaaagctg
     4141 gaaatgatgg tggcgaaagc agaagcagat gaaagagacc aggtatgaca accactgaat
     4201 gcatttttct ggcagcgggc ttcatattct gtgtgcttat gcttgccgac atggggcttg
     4261 ttcagtgaca cctcagcagg aaaacgccct tcgcagcatt gcccgtcagg ctaattctga
     4321 aatcaaaaaa gccagacagc agtttccgga taaaaacgtc gatgacattt gccgtagcgt
     4381 actgaagaag caccgcgaaa cggtaacgct gatgggattc acaccgactc atttaagcct
     4441 ggcaatcggc atgttaaacg gcgtttttaa ggaacggtga acatgaaaag caaaatcatc
     4501 agggagctac aggctccttt tttattattc gcatttaccc tcaagcgtat taaccaacaa
     4561 ttcagggatt aatgaaagat ggcggacatc attgattcag catcagaaat tgaagaatta
     4621 cagcgcaata cagcaataaa aatgcgtcgt ctgaactacc agactatatc cgccactcat
     4681 tgttgtgagt gtggcgatcc catagatgaa cgaagacgcc tggtcgttca gggttgtcgg
     4741 acttgtgcaa gttgccagga ggatctggag cttatcagta aacagagagg ttcgaagtga
     4801 gcgaaattaa ctctcaggca ctgcgtgaag cggcagagaa agccggtgaa gataagtggc
     4861 aggctaaaaa aataaatggt gattttttcg ttattcgtca cggtagttat acaagacagc
     4921 atggctacac atcgtatcaa cccattgcgg agattgattg taagccagtc cgggattttg
     4981 ttgccaaggc taatccggct accgtgctgg aattactgga tgaactggaa gcagcaaaaa
     5041 agcgcattgc agaactggaa gcgcgggaaa tactgctccc ggaacgtagc agcatgcttc
     5101 atcgaacaga ttttcacgat gattaccaaa cggtaatggc atacaaagtt tctgaagtca
     5161 tcgatgcaat ccgcgctact ggcattcgca tcaaaggaga gtgagatgat tcactaccac
     5221 ggtgggccta ttactcctga tacgtgcgca atgagagcat ggaaagggcg acatgcgttt
     5281 atcagttttg cgcattcagg ccagatcaat ctcgcggctg aatactgtca gtcgttcgcg
     5341 ctggacaacg gtgcattcac cgcctggaaa gcagctggca aaaacaaaat cgactggagc
     5401 gattactacg agtttgttgc tcgctggaag aatcacccag gattcgattt tgccattatc
     5461 ccggatgtta ttgatggcgg agaggaggaa aatgatgcgc ttctgaatga gtggcctcac
     5521 ggaaaactag ctggcgttcc agtgtggcac atgaatgaaa gtgacgagcg atttattcat
     5581 ttgtgcaatg agtttccgcg agtggctatc ggtagttgtg gcgactatga cgtaaagcgc
     5641 ccaactcttg cggtagccag aatgaaagac ctgattcgtc acattgttga tgggcatggt
     5701 cagccggtta cgaaactaca tggattgcgc atgttaaatc cgctgatatt cacaaaatta
     5761 cccttagcca gcgcagatag tacgaacgtc gctcgaaaca tcggtattga taaagcctgg
     5821 tctggggctt atgcacctgc aagtaaagag acacgcgcag cattaatggt agaacggatt
     5881 gaggcacaca atagccctgg ttctcttgcg tattgtgaac aacgcgaccg ctttgaaatg
     5941 caattgcaac tagcacttta aggactaaca aatgaccact attaccaaag aacgtattga
     6001 attgttcatt aaaaatccgc ttgaaaacgg gcttactcgt ggcgaacaaa tggaactggc
     6061 acgaattgca ctggcatcac tggaacgcga actgattcgc cacgagcatg ccaaatggtc
     6121 tgactccaca tttggctgcg ttggccccat tggtccgctg aaacatctct caaaagaggc
     6181 actggaagcc gcagccgaac ctgacgatct cagcgagtgg gctgatatgc agttcctgtt
     6241 gtgggatgca cagcgccgtg ctggtatcag tgatgctgaa attaccgctg ctatggaaaa
     6301 taaattgaag atcaacatgg agcgccagtg gcctgaacca aaagatggtg agcctcgttt
     6361 gcacattaaa gaacccggca actctccggt aactccggat ggttggataa gctgtagtga
     6421 gcgaatgcct gaaatgggag agcgacaatg ctatgtgtta gcagctgact ttaaaaacaa
     6481 ctacccacca aacatcccca acactcaggt cggcgtatat ggcgactggt ttaatgatgg
     6541 caagcccact tgggatgacg gtgatggcga agacctgtat ctcaaagagg taacccactg
     6601 gatgcagcta ccagaaccgc cgcaggaggt gaaatgatga attggcctga agcattcacc
     6661 gcagttggag ttgcaatagc ggtggcattt attctgtatt cgcttttccg ctggggataa
     6721 aggaatgttc gctctgattc aacgtggtca gatatacacg gacagagccg gataccctgt
     6781 ggtgattact cgcagtactc agcactcagt gttctttcga cgcatggacg ggcgctccgg
     6841 acgggtacgc attggtgagt tcaacaacct gttcgaacat attgaccaac aggagtaccg
     6901 caaaattctg gcgggcactg agcaggaaat gcgcctgaaa aaattacgcg caatgcaacg
     6961 gaggtgatac atgcatacgg cttttgagtt ctgggttcgc aagacattcg gcaatcgcta
     7021 cgacctgacc cgtgatgtcg acggcttcta ctgccgtgaa gttgtgaaac gaatgtttga
     7081 cgtgtggttc cactgccgtg gatgaaagtt ttatgaggtt ggcatgcaga caatcatcta
     7141 tcagataacc cccagcaaat ggtgtacgga gagagtcctt attgcatcaa cagggctaaa
     7201 gcccggcacc atcgagcggg ccagaagaaa gtcatggatg cagggaaaag aataccgcca
     7261 ttacgctgta gaaggtgatc ctgggcatta cagtgaatgc ctgtacaaca tcgaagaaat
     7321 tatgcgatgg atcgaaaacc agaaacaacc aggtgccaaa aatgcaagtt ccggttaacc
     7381 tgttaatgct cctggacgtc tgggaggttt aatgagtaac gcatcatacc cgacaggcgt
     7441 tgaaaaccat ggaggatcac tccgtatatg gtttcactat aatggcaaac gtgtcagaga
     7501 aaacctcggt gttcctgaca cagccaaaaa ccggaagatc gctggtgaac ttcgcacttc
     7561 cgtttgtttt gcaatcagaa tggggagttt cgactacgcc gcgcagttcc ctaattcccc
     7621 taacctgaaa cactttggtc tgggaaaaag agagataacc gttaaggcac tttcggaaaa
     7681 atggttggac cttaagaaaa ttgagatttg tgcgaatgca cttaaccgtt accagtcagt
     7741 aattaaaaac atgttaccaa tgttaggtga aaaaaaactg gtttcatcca taacaaaaga
     7801 ggatttactt ttcgtaagga gagatttgtt gaccggttac caaaagcttt ctaatggaaa
     7861 gacttcttcc ataaaagggc gctcagtggt cacggtaaac tactatatga caaccatagc
     7921 tggaatgttt caatttgcaa cagataatgg ttatacctca ggaaacccat ttaacggtct
     7981 ggctccctta aaaaagtcca aggtaaaacc agatcctctc acccgtgacg aatttattcg
     8041 ttttattgag gcttgccgtc atcaacaaac aaaaaacctg tggattctcg ctgtatacac
     8101 gggtattcgt cacggggagt tggtatcgct ggcatgggaa gatatagacc ttaaagcaag
     8161 gactataacc atccgtagaa attatacaaa acttggcgaa ttcactccac caaaaaccga
     8221 tgcaggcacc ggaaggacaa ttcatctggt tcaaccagct attgatgctc ttaaaagcca
     8281 ggcggaaatg accatgcttg gaaagcaaca ttctgtagag gtgaagcaga gggaatatgg
     8341 gagaactgct gtgcataaat gcacttttgt ttttagtcct caggtaacaa aacagcagca
     8401 gttgtccgga cctcactaca aggttgactc catcagggag tcatggacaa gtatcttaaa
     8461 acgcgcaggt ctgagacaca gaaaatcgta ccaatccagg catacttatg catgctggtc
     8521 acttgccgca ggagctaatc ctagttttat cgcaagccag atgggccaca caaacgcaca
     8581 aatggtattc aatgtttacg gagcatggat gaaagacaac aatcacgaac agatagaact
     8641 ccttaacaaa agactatctg aaagtgtccc atgtatgccc cataagaaag cggggtaaaa
     8701 taaaaacttg caaaaccaat tggtttacct ttaatccctg tcacgttacg cgcgtggcag
     8761 aggcgttacg ggttgctgaa accgcaacgg acagacggcg gtcatcgact gttcaacgat
     8821 gccgatattg accgtatccg cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc
     8881 aaagttaaaa tgctgctcag taatgaaaat gttgatgtgc agaacggctg gcgcgatcag
     8941 caagaaacat tactgaccta cctgcaaagc ggcaatctgc atagcctgcg aacgtggatc
     9001 aaagagcgcg gtcaggatta ccccgcccag acactcacca cacatctgtt tattcctctg
     9061 cgccgacgac ttcaatgcca acaaccgact ctccaggcgc tgctggcgat cctcgacggc
     9121 gtactgatca actacatcgc catttgtctg gcttcggcac gtaaaaaaca gggtaaagat
     9181 gcgctggtgg ttggctggaa tattcaggat accacccgtc tgtggctgga gggctggatt
     9241 gccagtcaac aaggatggcg cattgatgtc ctcgcccact cgctcaatca actacgccct
     9301 gaactattcg aaggccgtac attgctggtg tggtgcggtg aaaatcgaac ctccgcccaa
     9361 cagcagcaac tcaccagttg gcaagaacaa ggccatgata ttttaccact cggcatttaa
     9421 tgattcgtta acaaatgcgc tttactgtac aatcctttcg ttaacataag gagtgcatta
     9481 tgcgcatagc taaaattggg gtcatcgccc tgttcctgtt tatggcgttc ggcggaattg
     9541 gtggcgtcat gctcgcaggt tataccttta ttttgcgtgc tggctaagcg cctgcaccag
     9601 cctttcaaac aggcggtctg cgatgatcgc cgccagtgcc accagtaacg ccccctggat
     9661 cacatacgcg gtattaaatc cgctaagccc gatgatgatg ggcgtaccca gcgtgctggc
     9721 ccctaccgtt gaggcgatcg tcgccgtacc aatgttgata atcaccgaag ttcgcacgcc
     9781 cgccagaatc accggagccg ccagcggtag ctcgacctta cgcagtcgct gaccacgact
     9841 cattcccata cctttcgcaa cttctgtcac gctggcatcg atcgctccca gcccggcaag
     9901 tgtcgcctgc aggacgggca gcacaccgta aaggatcaag gcgataatcg ctggttgcag
     9961 accaaagccg atcaccggaa cggcaatcgc cagcactgcg acaggcggaa aagtctgtcc
    10021 aacggcggca atagtttcca ccagtgggcg aaattccgcg ccccacgggc gagtgacagc
    10081 aattccggca ccagtgccaa tgatcaccgc aaacaaactc gaaattccca ccagccagaa
    10141 atgagccagt gccagagctg caaaactttc ttgctgataa acgggtcgtg gcagttgtgg
    10201 gaacaaggca gcaaacagcg gctggctgta aggcagccag aaaatcagtg ccacaaacaa
    10261 agcaatgagc cagaacagcg gatcgcgcaa catcttcata cgcttacgcc tccaccagca
    10321 gatcctgaaa atgcagcgta ccgcaaggct ggccctgcgt gttcaccacc ggcagcacct
    10381 cgcaaccccg cgcgacaaac agggagagcg catcgcgtag cgtcatctct tctaccagtg
    10441 cctcaccttc tgcccgttct tcgcgacgca cgtaatccgc cacactacgt aacgaaagca
    10501 gacgcacacc cagttcgcta cgtccaaaaa actggcggac aaaatcattc gccggacgag
    10561 tcagcatcgt cagcggattg ccctgctgca ccacttcacc gtgatccatc aataccagat
    10621 gttcagccag ccgtagcgcc tcatcaatat catgagtgac cagcacaata gtgcgcccca
    10681 gcaaacggtg aatgcgcgtc atctcttgtt gcaacgcgcc gcgcgttacc gggtccagtg
    10741 cgccaaaagg ttcatccatc agtaagactt gcggatcggc agccagtgcg cgcgccactc
    10801 ccacacgttg ctgctgacca ccggaaagct gatgcggata acgctcacgc aaatttgact
    10861 ccagccccag tagcgtcatt aattcgtcga tacgatcgtc aatccgtgcc cgcgaccatt
    10921 tttgtaattg cggcacggta gcgatgtttt gcgccacgct ccagtggggg aacaggccaa
    10981 tagattgaat ggcatagccc atccggcggc gcaactccag tactggcagc gagcgaattt
    11041 cttctccggc aaagcggatc tctccgctgt catgttccac caggcggtta atcattttca
    11101 aggtggtgga tttgccggag ccagatgtgc caatcagcac cgaaaaactc ccttcctgaa
    11161 aattgagatt aagatcgtta acggcttttt gtgcgccaaa cagtttgctg acatggctaa
    11221 attcaatcat tacgtttcac cttcagcagt gcgataagca aatcgaacag ggcatcgatc
    11281 agcaccgcca gaacaattac cgggatcacc cccagcaaca ctaaatcaat ggcgctgctt
    11341 agcagcccct ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc
    11401 gccataccta cagtttgcac catcaccacc cgcaggctgc gcagaaatac cggtaacgcc
    11461 agtggtaact gaacatgcag gaatcgctgc gccccgctca tgcccatcgc tctggcgctc
    11521 tccagcacat cgcgcgggat ctggttcaaa ccgactacca cgccgcgcac cagcggcagc
    11581 aaggcataga gcaccagcgc aatcagtgcg ggtgtcattc cggttcctgc tatgccgagc
    11641 ttccccagcc acggaaaggc cgtcaccagc gcggcaagcg gcgcaatcaa caggccaaag
    11701 agcgccaccg aaggcacggt ctgaatgaca ttgagcagag aaaaaattgc cccctgccgc
    11761 gcagtggaaa agtagcacca gatgcccaac ggcacaccaa tcactaacgc aggcagcacc
    11821 gcaccaaaca gcaacgtcag atgttgtgcc agcgcgtcgt caaacacatc ctgacggttg
    11881 gcgtattctt tcattagtga gagatcgtta agcgtgccgg agtacagcaa ccacagcgga
    11941 ataatggcaa tctgcatatg caacaaccag cgccacagcg gatgcgtgga gattcggcgg
    12001 atggcatcgc tacaggccag caatgccagc gccgcagcca gccagaaacc actgccgagg
    12061 ctggtacgcg ccagcgcact gccattttgc gccagttggg tcgccgcctt tccagctccc
    12121 cacaccaaca atacgaagac gaattgcgcc agaatgagtg cacaaatgct gcctttttta
    12181 gcggggacaa aacaggctgt cagccaggcg caaccaacgc ccaccagcat ccagatcgtt
    12241 tgcggccaca gttgccagag atgacgccct tcacccgaga ctaaacgatt aggcgcgtag
    12301 ctgataaacg gcagcgctgc tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga
    12361 ttaatacgta gataagtcac gggaaattac ttcgcccacc ctttttgttt caggtagtcg
    12421 gcagccactt ttttggcatc cagtccttcc actgcaatgc tggcattcag ttgctgcaat
    12481 gtttttgcat cgaggctggc gaagactggc tgtagccact gtgccatttg cggatactcc
    12541 ttcaacaccg attcacgcac cactggtgca ggcgcataga taggttgcac gccttgcgga
    12601 tcgcttaagg tttgcagccc cagcgccgct accgggccgt cagtgccgta agccattgca
    12661 gcattaacgc cagaggtttg ctgggcagcg gctttgatcg tcaccgccgt gtcgccacca
    12721 gccagtgaca gcaactgatc ctgaccgagc ttaaagccat aggctttttc aaacgcgggt
    12781 aacgcatcgg cgcgttcgat aaactctgcc gaggctgcca gtttgaaggt gccgccctct
    12841 tgcagataac gactcaggtc ggcaagcgaa gtgagtttat ttttctctgc cacatcctga
    12901 cgcacggcga tagtccaggt gttatttgca ggcgcgggcg tcagccagat taacttgttg
    12961 tgctccgaat cgagcttttt gactttctcg taaccttgct gcgcgttttt ccacgctgca
    13021 tcgttttcat ctttaaagaa gaaagcgcca ttgccggtat attccggata gatatccagt
    13081 tcaccggaag taatcgcccc gcgcaccaca ggagtcgttc cgagttgcac tttattcacc
    13141 gtcggtacgc cgtggctttc cagcacctgc aaaatgatat tgccgagtag cgcaccttcg
    13201 gtatcgattt ttgaaccgac tttaacgggg gaagccgcct gtagcggcag gctcacggct
    13261 gccaacataa ccagtgaacc tgcccagagc tttaagagtg gcatgatgct ttcctcattc
    13321 ttttactgtt gttttcagcg aattaagaga aaagcatagt tgataatggc ggggttagct
    13381 tgagcggaag gattcagttg cagaatcaga taaatactta agaggcatat tcggtccggc
    13441 gtttcctgcc ggatgcggcg cgagcgcctt atccggccta caaagggcgc aaacgtcgcg
    13501 cccttactaa agcattacag caactcaaac tcgcctttct taacgcgtgc ggaatcagtg
    13561 ccgataaata cattgaactt gccaggctcg gcgtcatatt tcatctgttg attccagaac
    13621 ttcagcgcct caatatcgat cgggaagctg acagtctgag tttcgcccgg tttcagggtg
    13681 attttctcaa agcctttcag ctgtttcaca gggcgactca tggaagccgt cacatcctgc
    13741 aagtacatct gcactaccgt ggccccctcg cgcttaccgg tgttcgtcac ctgcacgctg
    13801 gcagtcactt tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc agagacggtg
    13861 aaagtggtgt agctcagccc atagccgaac ggatacaacg ccccgttagc ttcatcaaaa
    13921 taacgcgaag tgtatttgtt cggcttgtcg gcattatacg ggcgaccggt attcagatgg
    13981 ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg acattggcag cttgccggac
    14041 gggttgtaat cgccaaacaa tacatcggca attgcattac cgccttcagt ccccgcaaac
    14101 caggtttcca gaatcgcatc agcctgctga tcttctttca ccagcgccag cggacgcccg
    14161 ttcatcagca ccagcaccag cggtttaccg gtggctttca gcgccgcaat caagtcacgt
    14221 tggctttgcg gaatagtgat atcggtccgg ctggaggctt cgtgcgccat cccctgtgct
    14281 tcaccgacta cagccaccac cacatcagat tgtttcgccg tctgcaccgc ttcatcaatc
    14341 atctcttgcg gcgaacgcgg atcgactttg accgcttctt catactgatt caggaaatcg
    14401 ataatgcctt tgtcactggt aacgttcgcc cctttggcat acagcacttt accgttttca
    14461 ccgacggaat ttttaatccc ggtcagtacg gtcacggatt gatcggcaac accggctgcg
    14521 gaccagctgc ccatcacgtc acgtttactg tccgccagtg gcccaaccac cgcaatggtg
    14581 gccgattttt tcagcggtaa cgtttcgaga cggtttttca gcaacaccag gctttcgcgc
    14641 gccacttcac gcgcttcttt acggtgcagg cggctttcgg cattggtatc caccgggtca
    14701 gactctttcg gccccaaatg gctgtatggg tcgttaaaca atcccatatc atatttaacg
    14761 ttcagtacat ggcgggcagc atcgtccagc tcttccatcg tcactttgcc ggatttgatc
    14821 aacccaggca gatacttcga gtaatactcg tcgctcatac tcatgttgat tccggatttc
    14881 agcgccacgc gcaccgcatc ttccgggtct gccgccgtgc catgtttaat cagctctttg
    14941 attgcaccgt gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg cagaacatct
    15001 ttcagcagcc aggaatcgga ggtggctggc gtgccgttca gcgagttcag cgccaccatc
    15061 accgcgccgc tgcctgcgtc cagccccgct ttgtacggcg gcatataatc attaaacagg
    15121 cgctgcggac tcatatcgac ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg
    15181 gcaaagtgtt tgacgctggt catcaccgag tagcgatctg ccgggctttt accctgcatc
    15241 gcttccacca tggttttacc cattgttgag gtgagatacg tatcttcgcc aaaaccttcg
    15301 gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca tcggtgccca ggtcatattc
    15361 aggccatcat ctgccgcttc ataagcagag acacgtccga ccgttttcac tgcatcgagg
    15421 ttaaaagacg aggccagacc gaggctaatc gggaacaccg tgcgctgacc gtgcagcacg
    15481 tcgtaagcaa agaaaagagg aattttcagg cggcttaatt ccatcacctg atcctgcatg
    15541 gcgcggatat cctgacgggt tacggtgttg aaaatcgccc caacctgacc gtctttgatc
    15601 atctcgcgga tcgcctcttt cgggttatcc gggccgacgc tgattaagcg cagctgacca
    15661 attttctcat caactgtcat tttcttaagc agttcggtga cgaacgcatc ccgcgcttcg
    15721 ggcgttaatg gatggttgcc gaataaatca tccgccagtg ctggctgcag ggccagactc
    15781 accgcgattc ctactgaaca tagccatttc atatggattt ttcttcctct gttgcccgac
    15841 gaggcagcga aaatggtgca aaaaccgtag tttgccataa gcatgatgga gagagaaaaa
    15901 gaatgctcag tttattgtct gaattttcaa aatattcact cgctgaattg tcatacaagg
    15961 cgctattcta gtttgtgata ttttttcgcc accacaagga gtggaaaatg tcttccatga
    16021 caacaactga taataaagcc tttttgaatg aacttgcccg tctggtcggt cattcacacc
    16081 tgctcaccga tcccgcaaaa acggcccgct atcgcaaggg cttccgttct ggtcagggcg
    16141 acgcgctggc tgtcgttttc cctggctcac tactggaatt gtggcgggtg ctgaaagcct
    16201 gtgtcaccgc cgacaaaatt attctgatgc aggctgccaa tacaggcctg accgaaggat
    16261 cgacgccaaa cggtaacgac tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg
    16321 acaagctgca cgttcttggc aagggcgaac aggtgctggc ctatccgggc accacgctct
    16381 attcgctgga aaaagccctc aaaccgctgg gacgcgaacc gcactcggtg attggatcgt
    16441 cgtgtatagg cgcatcggtt atcggcggta tttgtaacaa ctccggcggc tcgctggtgc
    16501 aacgtggccc ggcgtatacc gaaatgtcgt tattcgcgcg tataaatgaa gacggcaaac
    16561 tgacgctggt gaaccatctg gggattgatc tgggcgaaac gccggagcag atccttagca
    16621 agctggatga tgatcgcatc aaagatgacg atgtgcgtca cgatggtcgt cacgcccacg
    16681 attatgacta tgtccaccgc gttcgtgata ttgaagccga cacgcccgca cgttataacg
    16741 ccgatccgga tcggttattt gaatcttctg gttgcgccgg gaagctggcc gtctttgcgg
    16801 tacgtctaga taccttcgaa gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc
    16861 agccggaagt gctgaccgaa atccgccgtc atattctggc taacttcgaa aatctgccgg
    16921 ttgccgggga atatatgcac cgggatatct acgatattgc ggaaaaatac ggcaaagaca
    16981 ccttcctgat gattgataag ttaggcaccg acaagatgcc gttcttcttt aatctcaagg
    17041 gacgcaccga tgcgatgctg gagaaagtga aattcttccg tccgcatttt actgaccgtg
    17101 cgatgcaaaa attcggtcac ctgttcccca gccatttacc gccgcgcatg aaaaactggc
    17161 gcgataaata cgagcatcat ctgctgttaa aaatggcggg cgatggcgtg ggcgaagcca
    17221 aatcgtggtt ggtggattat ttcaaacagg ccgaaggcga tttctttgtc tgtacgccgg
    17281 aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca attcgttatc
    17341 aggcggtgca ttccgatgaa gtcgaagaca ttctggcgtt ggatatcgct ctgcggcgta
    17401 acgacaccga gtggtatgag catttaccgc cggagatcga cagccagctg gtgcacaagc
    17461 tctattacgg ccattttatg tgctatgtct tccatcagga ttacatagtg aaaaaaggcg
    17521 tggatgtgca tgcgttaaaa gaacagatgc tggaactgct acagcagcgc ggcgcgcaat
    17581 accctgccga gcataacgtc ggtcatttgt ataaagcacc ggagacgttg cagaagttct
    17641 atcgcgagaa cgatccgacc aacagcatga atccggggat cggtaaaacc agtaaacgga
    17701 aaaactggca ggaagtggag taaaaattac ggatggcaga gtatcgccat ccgaattcac
    17761 ttaatcgttc tgtgccgtct gccccgccgc cgccatttgg gcggcttttt gttttttata
    17821 gctcaacgct gctgccggca caggcatcac tttaccggtt tcaatccagg tacgcaggcg
    17881 gctggcatcg gcaaaatggg tatatttgcc aaacgcgtcc atcactacca gcgccaccgg
    17941 tttattattg ataaccgtac gcatcaccag acaatggccc gccgcattgg taaagccggt
    18001 tttggttaac tgaatattcc agttatcgcg atacaccaga tgattagtat tgcggaacgg
    18061 cagcgtatac gtcgggttag agaaggttgc catatcttcc cgggtagtac ttaactgccc
    18121 gatcaacgga tattgtttgc tggcaatgag cagtttggtt aagtcacggg cagttgaaac
    18181 gttatgcacc gacaatccgg taggttcaac aaagcgcgtg ttgttcattc cgagcgattt
    18241 cgctttcgca ttcattgcct taataaaggc tttgtaacca ccgggataat ggtgcgcaag
    18301 gcttgccgcc gcgcggtttt ctgaagacat cagcgccagc aacagcatat ctttacggct
    18361 gatttcgcta ttcagtcgta cgcgcgaata gacccctttc atctccggcg tctggctgat
    18421 atccactttt agtttttcat ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat
    18481 taatttgctg atagacgcaa ttggacgcac cagatccggg tggttcgaat agatcacttt
    18541 gttggtattc agatcaacaa tcatcgcgct accggaggca atttccggtt gtgaagcggc
    18601 ggtagcggct gccgttttcg caacggcctg cggtgccaaa ggcacagcca gcatcagggc
    18661 caggctaaat aaagaaactc gaaatttcgg catgatgagc attcagatag tggttcacgc
    18721 gcacgggttg cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc atcataacga
    18781 gcaaaaagtg cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct gacatttacg
    18841 ccagcaatgc aatgtcaaag aactttctag aacaggcgat acccgtagcc ccatagtata
    18901 acggttaggg cgagcagcac ttccagtacc agcacgccaa tcgccagcgt cgaactggag
    18961 aagctaaggc cttcctcttt gttgatattc aggaagctcg gaataccaag gtaaagcagg
    19021 tagccggtgt aaaacagcgc caccgtgccg accagcgcac acaaccagac cagcggataa
    19081 agcgccacca gaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag
    19141 tgcgcaagtg acggacgctg cgggtaattg cgcgccatcc accagatgac ccgccccatc
    19201 accgcgaccc cagccagcat aacgccgtaa aacaagacag ccagcgccag tccggtaaac
    19261 caggataact tcaggatagt gccatcgcca aaattccagc caatctgtgt agtgccaatg
    19321 aaggcgcaaa tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa
    19381 atcgtttcgt tttcgcgatt aatcacctgc atttcacgat cgggatggga aaacagtccc
    19441 cagacatggc tcataccgcc cccttgttgt gagttcatga acctgacagt tcaagtataa
    19501 gtcagcttgt gattattttt tgttcgccca tgtaattttc actgtctgat gaacgtcctt
    19561 tttcagcgtg aatgattcac agggtgtatg cttactggca accaaaggga gacagactgg
    19621 cctatggatc tcaatacact tatctcacaa tatggttatg ccgcgctggt gatcggtagc
    19681 ctggcggaag gtgaaaccgt gactttgctg ggaggcgttg cggcacatca ggggctatta
    19741 aagttcccgc tggtggtact ttctgtggcg tttggcggca tgattggcga ccaggtgctt
    19801 tatctgtgcg ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa acatcaggat
    19861 aaaattgagc gggcgcagaa acttatccaa cgccatccgt atctgtttgt cattggtacg
    19921 cgctttatgt atggctttcg ggtgattggc ccgacgctga ttggtgccag ccagctgccg
    19981 ccgaaaatct ttctgccgct gaatattctc ggcgcatttg cctgggcgtt gatttttacc
    20041 actattggtt acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat
    20101 ttgaagcact gggtctggtt gattctggtc gtggttctgg tggtgggcgt gcgctggtgg
    20161 ctgaaacggc gcgggaagaa aaagccggat catcaggcgt aaaaccattg ccctgggtaa
    20221 ggcgttcgcg ccgcatccga catccggtgc accatgcctg atgcgacgct tgtgcgtctt
    20281 atcatgccta ccaaaatttt acccattgaa ataaccacgc acctcactgc gctattctgg
    20341 gttgaactgc ggattcgcca acataaagcc gccatccact atcaacgact gcccggtggt
    20401 gtaatttgcg ccctctgagc acagccacgc taccaggctg gcaatctcat gcgttgcgcc
    20461 aaaacgccgc agaggaatcg aaggctccgc atcgggcttc acgtcgctgt catccatgcc
    20521 attcattggc gtggcgatcg ccccaggcgc gactgcgttc accaaaatct tatgcctgac
    20581 cagctccagc gccatcgttt tggttaaccc gccgagcgca tgtttagcgg ctgtgtaagc
    20641 gctggcatcc ggcagcggcg tatgttcatg taccgacgta atgttgatga tgcgaccgcc
    20701 ctgcccttgt ttcaccatct ggcgggccgc aatttgtgag cataaaaatg caccatcgac
    20761 atcaacggta aaaatcttgc gccactcatc gaaagccata tcaagaaacg gcgctttggt
    20821 cattgcgccc gcattattca ccagcacatc aatacgcccc agccgttgaa tgagtttctc
    20881 cagcgcctgc gcaccttctg gcagtttacc gagatccagc tgcacgatct ccgcacgtac
    20941 gccgtggcta actacctcac gcgcggtatc ttttgcccct tcttcatctg agtgccaggt
    21001 aatgccaata tcaaacccct gctgcgccag taataacgcg cactctttgc cgatccccga
    21061 atcggaggcg gtaataatcg caacctgtgc catcgagttc tccacttaac gctgaataaa
    21121 cgttaagtat agaaggcgca tatcatcagc gtttgtaacc cccgcccaac gcaccagtga
    21181 gttgaatgga agcatccagc cactgccctt gcaataacag gccattggcc cgctcacgca
    21241 gcgcggggat tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg
    21301 cctgcgcaag acctaccaca cgcaaggcat cgcgctcaat ttgcgcctga tgctggtttt
    21361 tctccgcaag tgtctgaacc tgactggctg cccgcgccac gtcattcacc gcttcaacca
    21421 ccgctttgtt gtagctggcg atagacaagt tgcttccggc ttttgcgata tcgagattgg
    21481 cgttaagacg accactatcg aaaatgggta gcgtcaggcc tgccgtaacg cccatttgct
    21541 gcgcggaatg acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca
    21601 ggttgatgtc aggataaaat gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa
    21661 cgtaccagtg cgccgcctgc aaatctgccc gccgggccag taaggagtac cccagttcat
    21721 caggaagctg gcttgccact ttcggcaacg cgaccggttt aagcttcaat gactttgtct
    21781 ggttatttgt aagtgcgctt aaccgtgcct caataatttt cattttcccc gcgacatcgt
    21841 tgagctgctg ccgggttttg ctggcattaa tatcggtttc cacaccgtca actgaagaag
    21901 taatcccgtt ctgatatagc tggcgatcgg tcgcgataat ggtgttctgc tctttttcta
    21961 tttgctgcaa gaccgtgttt aacgccgcct gggtttgcca ctcccagtac aggcgggcta
    22021 cgctgccagc cagcaattgg cgggtttgct cgcgttccgc cgcccgtgct ttaaccgtac
    22081 ccaggcgggc agtaacctcc gcccgattct ttccccagat atcgagatgc cagcccgccg
    22141 ttaagccaaa agttccgttg gtgtaccacg gaccggtcgt acctgcggcc ggatcgttca
    22201 gagcaaacgg ccccattaag ccttctgccg acattttttg ccgctccata tccgcagaaa
    22261 agtcgatctg cggaccatcc tgagtagcaa ttgccttcgc ctgggcttca gctagctgaa
    22321 tgcgctgttc agccacctgc atatccggtg cgttctgtag tgcattgtta attaaggaag
    22381 tgagttgatt atcgtgatac tccagccacc attggctgtc tggccaacca tttttcagcg
    22441 ccgtgggtaa tgcggtgtca acttgtgcag cgggcgtttg ctggcttaac gcctggcggg
    22501 tttcatgcat aggcgcacac ccggccagca tcagtaacag cggaaaacag gcgatggcgg
    22561 gataaaagga atcacgattc atgggggaat aatcaggtaa gaaaaggtgc gcggagatta
    22621 ccgtgtgttg cgatatattt tttagtttcg cgtggcaata catcagtagc aataaaacga
    22681 catatccaga aaaatataca ctaagtgaat gatatcttcc gatttatctt aatcgtttat
    22741 ggataacggc aaagggcttc gttttttcct atacttattc agcactcaca aataaaggaa
    22801 cgccaatgaa aattatactc tgggctgtat tgattatttt cctgattggg ctactggtgg
    22861 tgactggcgt atttaagatg atattttaaa aatcagatat ggcatataag ttgtcaggtc
    22921 aggaactaga tattttgtgt acttgatttg caaacagctc cggtgttggt ggcgtcaaaa
    22981 tcgctgaagc gttccccgaa ggccggggca gcccac
//
LOCUS       DAZFYC010000074        22909 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFYC010000074 DAZFYC010000000
VERSION     DAZFYC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.074"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..517
                     /gene="ybeT"
                     /locus_tag="ACHKW5_003207"
     CDS             <1..517
                     /gene="ybeT"
                     /locus_tag="ACHKW5_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7512549.1"
                     /translation="VAAITCISQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAATELS
                     AIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLG
                     VKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKN
                     AERLLAGLPAH"
     gene            complement(527..1954)
                     /gene="djlB"
                     /locus_tag="ACHKW5_003208"
     CDS             complement(527..1954)
                     /gene="djlB"
                     /locus_tag="ACHKW5_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO7512550.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1951..2658)
                     /gene="ybeR"
                     /locus_tag="ACHKW5_003209"
     CDS             complement(1951..2658)
                     /gene="ybeR"
                     /locus_tag="ACHKW5_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7512551.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2822..3799
                     /gene="ybeQ"
                     /locus_tag="ACHKW5_003210"
     CDS             2822..3799
                     /gene="ybeQ"
                     /locus_tag="ACHKW5_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO7512552.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3869..4351)
                     /gene="ybeL"
                     /locus_tag="ACHKW5_003211"
     CDS             complement(3869..4351)
                     /gene="ybeL"
                     /locus_tag="ACHKW5_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO7512553.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFIRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4586..7168
                     /gene="leuS"
                     /locus_tag="ACHKW5_003212"
     CDS             4586..7168
                     /gene="leuS"
                     /locus_tag="ACHKW5_003212"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO7512554.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7183..7764
                     /gene="lptE"
                     /locus_tag="ACHKW5_003213"
     CDS             7183..7764
                     /gene="lptE"
                     /locus_tag="ACHKW5_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO7512555.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7764..8795
                     /gene="holA"
                     /locus_tag="ACHKW5_003214"
     CDS             7764..8795
                     /gene="holA"
                     /locus_tag="ACHKW5_003214"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO7512556.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8797..9438
                     /gene="nadD"
                     /locus_tag="ACHKW5_003215"
     CDS             8797..9438
                     /gene="nadD"
                     /locus_tag="ACHKW5_003215"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO7512557.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9462..10073
                     /gene="cobC"
                     /locus_tag="ACHKW5_003216"
     CDS             9462..10073
                     /gene="cobC"
                     /locus_tag="ACHKW5_003216"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO7512558.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            10333..10650
                     /gene="rsfS"
                     /locus_tag="ACHKW5_003217"
     CDS             10333..10650
                     /gene="rsfS"
                     /locus_tag="ACHKW5_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO7512559.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10654..11121
                     /gene="rlmH"
                     /locus_tag="ACHKW5_003218"
     CDS             10654..11121
                     /gene="rlmH"
                     /locus_tag="ACHKW5_003218"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO7512560.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11152..13053
                     /gene="mrdA"
                     /locus_tag="ACHKW5_003219"
     CDS             11152..13053
                     /gene="mrdA"
                     /locus_tag="ACHKW5_003219"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO7512561.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13056..14168
                     /gene="mrdB"
                     /locus_tag="ACHKW5_003220"
                     /gene_synonym="rodA"
     CDS             13056..14168
                     /gene="mrdB"
                     /locus_tag="ACHKW5_003220"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO7512562.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14179..15267
                     /gene="rlpA"
                     /locus_tag="ACHKW5_003221"
     CDS             14179..15267
                     /gene="rlpA"
                     /locus_tag="ACHKW5_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO7512563.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPLPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15407..16618
                     /gene="dacA"
                     /locus_tag="ACHKW5_003222"
     CDS             15407..16618
                     /gene="dacA"
                     /locus_tag="ACHKW5_003222"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO7512564.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16729..16992
                     /gene="ybeD"
                     /locus_tag="ACHKW5_003223"
     CDS             16729..16992
                     /gene="ybeD"
                     /locus_tag="ACHKW5_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO7512565.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17093..17734
                     /gene="lipB"
                     /locus_tag="ACHKW5_003224"
     CDS             17093..17734
                     /gene="lipB"
                     /locus_tag="ACHKW5_003224"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO7512566.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIVPRLLENILALLNNPDFEYITA"
     gene            17993..18946
                     /gene="ybeF"
                     /locus_tag="ACHKW5_003225"
     CDS             17993..18946
                     /gene="ybeF"
                     /locus_tag="ACHKW5_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO7512567.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINLTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19155..20120
                     /gene="lipA"
                     /locus_tag="ACHKW5_003226"
     CDS             19155..20120
                     /gene="lipA"
                     /locus_tag="ACHKW5_003226"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO7512568.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20221..20424)
                     /gene="tatE"
                     /locus_tag="ACHKW5_003227"
     CDS             complement(20221..20424)
                     /gene="tatE"
                     /locus_tag="ACHKW5_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO7512569.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20553..21341)
                     /locus_tag="ACHKW5_003228"
     CDS             complement(20553..21341)
                     /locus_tag="ACHKW5_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO7512570.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGELLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21434..21817
                     /gene="crcB"
                     /locus_tag="ACHKW5_003229"
     CDS             21434..21817
                     /gene="crcB"
                     /locus_tag="ACHKW5_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO7512571.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21872..22081)
                     /gene="cspE"
                     /locus_tag="ACHKW5_003230"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21872..22081)
                     /gene="cspE"
                     /locus_tag="ACHKW5_003230"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7512572.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22256..22816)
                     /gene="pagP"
                     /locus_tag="ACHKW5_003231"
     CDS             complement(22256..22816)
                     /gene="pagP"
                     /locus_tag="ACHKW5_003231"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO7512573.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5876 a         5753 c         5678 g         5602 t
ORIGIN      
        1 agttgcagca attacctgta ttagccaacc caagaaaaca acgttgcgtg ataaagccat
       61 ggtgaattat gcctttgatt atttaagctc accaggcagt ctaccgttaa ccacggcagc
      121 cacggagctt tccgcgattc atggtcactc aacatcgcaa tatcgccttg gcgaatttta
      181 tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca
      241 atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg gggtggatct acctgaaagg
      301 cctgggagtc aaacccgata cccgtaaagc aattctctgg tataaggaag cagctgaaca
      361 agggtatgct catgctcaat atactttggg tttgatctac agaaatggca ctggtattaa
      421 tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac attacaaaaa
      481 tgcggaaaga ttacttgccg ggcttcccgc acattaacgt cgactgtcaa ccgaacagat
      541 ggagtatttt ccctatcaaa ccagcaaaga taaaaatata gaaaataatt ttaatgatgc
      601 ccagacagcc tggcccacca tcgtcaacgg gcattggctg cggttgcggc tccggttcaa
      661 cagtttgctt ctcggtaaaa ttcgccgcct gaattaataa tcgacgaata attaatgctt
      721 ccagtggaca acgatcttct gcatcaaggc ggctcacaat cggcattaat gattcttcgg
      781 ttggttggtt cgcctgcact aattgtttgg caactaaata taatggccat aagggatgtt
      841 ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc atctcgacca
      901 gtgaacgccc cagagcagag agtttttcat cgctgagtat ttcgtataaa tgaaaacgcg
      961 tacgggcgct gtcgccgggg atataaagta gcggttttcc ctgatcgtta accagttcac
     1021 gagcttcaat catcatcacc actaacgggt aataatcgac ccgatgctga cggcaccatt
     1081 gcaatattaa agcatccgcc tgggtggagg gatactcgcg ccagtagtca caaagttctg
     1141 ccagcaagct ttcgccttct ccacaataga cgcgttgtgc gtattcataa taacgcgcat
     1201 cttcattatc agattcagct tcccgccacg accgggcgta atccaggagt tcgggaattc
     1261 ccaggcgacc tgcgctatac cagcggagta aatttctgtg cagttttgca tcatcgggga
     1321 taatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc gatgaccaaa
     1381 tccgagcgaa catttggtaa aaatcgatgg tgtcattttg cacggcaacg ggcagatgca
     1441 gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag gattccagtt
     1501 cttcttcgtc tatttccccg acgttttcct cctgcaacca ccgcaatctt tctgctaata
     1561 acaccacgca ctcgaaggat aaatgggcag tgttcatggc gattgtgcac agcgaccagc
     1621 gtaattcatc aatctcttcc atcgagcaat aatttaattg ctgaatgaat cgctgccagg
     1681 cggagggcag aaaacgttcg ctatcagagg caagtaacgc acgaaaggcg agcagaattt
     1741 catgttctgc tacctcatat tcttctggtt gccaaacaga tttagcaggc gactgcgcaa
     1801 tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa accctgcgga tcggtttctg
     1861 gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgacg tcagtcgttt
     1921 cctctatttc gagaatcttc cagcaatttt tcataggtcg ctcatatcct taagcgtttc
     1981 cgggcatgca gaatagtaag ccaatggttg ccaggacaag gtagggtaat acgcatcatc
     2041 actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt tacgcgctaa
     2101 gaattcgcgt aataactgca gatgatcatc ttcgggagac tgccaatatt gccgaccaaa
     2161 ggtataaccg gcaaagtatt gccgccagtt acgataataa cgtagagccc gtagatgaat
     2221 gcgcgactgg atccatagac tttcttcttc gctcaaccag ttattaagta cgcccattcg
     2281 gcttaaaaaa cccatccgga cataatccca ggctttaatt cctccctcac cacagcattc
     2341 ggcggtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttagtt cagcaagata
     2401 gtcacgccat tcacatggcg aataacgaaa ccagcggtga taaaaccctg ccaaccgagc
     2461 ggcatgaccg ttatcgatca ttcggtgaat tgtgcgcagc aggtcatcac gggcatcaat
     2521 tccccaggag gaagaaaggt caaaaaagca tgtacctaaa tacattttgg gtgatgaatg
     2581 cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa acaatatttt
     2641 ttgcgactcc atgtccatat aatttatcct taaatattca ttaaatgaat attagccgca
     2701 ttgcgaggct cgtttattta tacttttctg acgcgacata atacgcataa aataaggctt
     2761 acgcaaaaat ggaacacata aaaatataat atgttttatc tgcaaccgta aggaaatgat
     2821 aatgattttc acgtcaagtt gctgcgataa tttatcaata gatgagatta tcgaacgtgc
     2881 tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc gcgatagcgc
     2941 aattgattct ccggacgacg aaaaagcctt ttactggctg aagctggccg ctgagcaagg
     3001 tcattgtgaa gcacagtatt ccttagggca aaagtatacc gaggataaaa gccgtcataa
     3061 agataatgag caagccatct tctggctgaa aaaagctgcc ctacaaggcc atactttcgc
     3121 ttccaacgcc cttggctgga cactggatcg tggagaagcc cccaattata aagaagcggt
     3181 tgtctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata atcttgggtg
     3241 gatgtacaga aatggcaacg gagtcgcaaa agactatgcg ctggcatttt tctggtacaa
     3301 acaagctgca ttacaaggcc atagtgacgc gcaaaacaat ctggccgatc tttatgaaga
     3361 cggaaaaggc gttgctcaaa acaagacact cgccgcattc tggtatttga aaagcgcaca
     3421 gcagggtaat cggcacgccc agtttcaaat tgcatgggat tataacgctg gcgaaggggt
     3481 ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc aggaaagcgt
     3541 cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg agaaagatta
     3601 tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta
     3661 taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc gacaggctct
     3721 cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg aaattcgtga
     3781 gactgaagat ttactgtaga gataaaaaca agggccgggg aatttcattc ggcccatatt
     3841 ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc tggaactggt
     3901 catgaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg agatggaagt
     3961 gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga taaaccccat
     4021 gatgattgag gtcctggaaa acttcgcgcc attcaagctg cgttttatcg gtgatatctg
     4081 ccagctcctg ccataagctt tctttaatca cccgtataaa gacgctgtca gattcttctt
     4141 tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtcttaca gctcgcgtca
     4201 gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt
     4261 ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca
     4321 gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact
     4381 ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta
     4441 atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg
     4501 gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat ttgtagccgt
     4561 attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc
     4621 caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa
     4681 agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca
     4741 cgtacgtaac tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa
     4801 cgtcctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa
     4861 aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct
     4921 caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta cgccggaata
     4981 ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg tatataagaa
     5041 gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat
     5101 cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc cgcagtggtt
     5161 tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg
     5221 gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat
     5281 caccttcaac gttaacgact atgacaacac gctgaccgtt tacactaccc gcccggacac
     5341 ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc
     5401 ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca aagttgccga
     5461 agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag ctgttcaccc
     5521 attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg agtacggcac
     5581 gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata
     5641 cggcctgaac atcaaaccag ttatcctggc tgctgacggc tctgagccag atctttctca
     5701 gcaagccctg actgaaaaag gcgtgctgtt caactctggc gaattcaacg gtcttgacca
     5761 tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg gcgagcgtaa
     5821 agtgaactac cgcctgcgcg actggggtgt ttctcgtcag cgttactggg gcgcgccgat
     5881 tccgatggtg acgctggaag acggtaccgt aatgccgacc ccggacgacc agctgccggt
     5941 gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag cagatccgga
     6001 gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca ctttcgacac
     6061 ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca aagaaggtat
     6121 gctggattcc gaagcggcta actactggct gccggtggat atctacattg gtggtatcga
     6181 acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg
     6241 catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga
     6301 tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg ttgatgctat
     6361 cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact
     6421 ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt
     6481 gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc
     6541 tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg
     6601 tgtctggaaa ctggtttacg agcacacagc aaaaggtgat gttgcggcac tgaacgttga
     6661 tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacga tcgctaaagt
     6721 gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct
     6781 gatgaacaaa ctggcgaaag caccaaccga tggcgagcag gatcgcgctc tgatgcagga
     6841 agcgctgctg gccgttgtcc gtatgcttaa cccgttcacc ccgcacatct gcttcacgct
     6901 gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga
     6961 aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc
     7021 caaaatcacc gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga
     7081 acatctggta gcaaaatatc ttgatggcgt taccgtgcgt aaagtgattt acgtaccagg
     7141 taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca
     7201 ttgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgatacc
     7261 acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca
     7321 ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct tgataaagaa
     7381 accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc
     7441 gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg
     7501 accgtgttga tccccggccg tgatatctac ccgattagtg ccaaagtctt ccgttcgttc
     7561 tttgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag
     7621 atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat
     7681 attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac gcctgcacgc
     7741 gtctccacca cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc
     7801 tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg
     7861 aaagccagga cgctgttcgt caggtagctg cggcacaagg attcgaagaa caccacactt
     7921 tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc
     7981 tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg aatgcggcga
     8041 tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgctg ttgatcgtcc
     8101 gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgct cttgcgaatc
     8161 gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttgctg
     8221 cgcgcgcaaa acagctcaac ttagaactgg atgacgctgc aaatcaggtg ctctgctact
     8281 gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg ctgctctggc
     8341 cagacggcaa attgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca
     8401 ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg ttgcatattc
     8461 ttcagcaact gcgtctggaa ggcagcgaac cggttatttt gttgcgcaca ttacaacgtg
     8521 aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt
     8581 ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct
     8641 taagtcagac gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca
     8701 aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt ctgttgtgcc
     8761 ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt
     8821 ggcggcacct ttgatccggt gcactatggt catcttaaac ccgtggaaac gctggcgaat
     8881 ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag
     8941 ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc cgacaagcca
     9001 ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca
     9061 ctgaaagagt ggcggcagga acaagggccg gacgtgccgc tggcgtttat tattggtcag
     9121 gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga caatgcacat
     9181 ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca ataccagcaa
     9241 tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa
     9301 atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg
     9361 caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tactgactta cattaaccaa
     9421 caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc
     9481 gtcatggcga aacgcaagcg aatgtcgatg gtctgtacag cggtcatgcg cccacacccc
     9541 tgaccgcacg cggtattgag caagcgcaaa atctgcatac gctgctacat ggtgtttcct
     9601 ttgatctggt tttatgcagt gaactggaac gggcacagca taccgcgcga ctggttctca
     9661 gtgaccgcca gctccccgtt catatcatcc ctgaactcaa cgaaatgttt tttggcgact
     9721 gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat agcgcgtggt
     9781 gcaatgactg gcagcatgca atccccacga acggtgaagg atttcaggca ttttcgcaac
     9841 gtgtggaacg ctttatcgca aggcttagtg aatttcagca ctatcagaat attttagtcg
     9901 tcagccatca gggtgtactg agtctgttaa tcgcccgttt aattggcatg cctgccgagg
     9961 ctatgtggca ttttcgcgtt gaccaggggt gttggagcac cattgatatc aaccaaaaat
    10021 tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaatgca tgacttttct
    10081 gtttttttac gggtaagccg caacgaccat tgacagaccc gggcaggctg atattctccg
    10141 cagccagact tttttcgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg
    10201 caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg
    10261 tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc
    10321 cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac
    10381 ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat caccgactgc
    10441 atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga ccacgttgtg
    10501 caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac
    10561 tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc
    10621 ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga
    10681 acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa
    10741 gatatgccct tcgagctgat tgaaattccg gccggaaaac gcggcaagaa tgcggacatc
    10801 aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt
    10861 gtcaccctcg atattccagg caagccctgg gatacgccgc agttagccgc tgagctggaa
    10921 cgctggaagc tggatggtcg cgacgtcagt ctactgattg gcgggcctga agggttgtcg
    10981 cctgcctgta aagcggcggc ggagcagagc tggtcgctgt cgacgcttac cctcccccat
    11041 ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac
    11101 catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg gatgaaacta
    11161 cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc
    11221 gcctttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta taatctgcaa
    11281 attgttcgct ttaccgacta ccagacccgc tctaatgaaa accgcattaa gctggtgcct
    11341 atcgcgccca gccgcggcat tatctacgac cgtaatggta tccctctggc cctcaaccgc
    11401 actatctacc agatagaaat gatgccagag aaagtcgata acgtgcagca aacgctggac
    11461 gctttgcgca gcgtggttga tctgaccgat gacgatattg ctgcattccg aaaagagcgc
    11521 gcacgttcac accgtttcac ctctattccg gtgaaaacta acctgaccga agtacaagta
    11581 gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt
    11641 cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac
    11701 gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat
    11761 gatatcggta agctgggcat tgagcgttac tatgaagacg tgctgcacgg tcagaccggt
    11821 tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca
    11881 ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa
    11941 acgctgctgg cgggtagccg cgcagctgtg gttgtcaccg atccgcgtac aggtggggtg
    12001 ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc
    12061 aaagattatt ccgccttgtt gaacgacccg aatacaccgc tggtgaaccg cgccacacag
    12121 ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attgagcgcc
    12181 ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact gccaggttcg
    12241 gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg
    12301 ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc
    12361 ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga cctggcggaa
    12421 gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg
    12481 tatcagggtg acaccattcc ggttggtatc ggtcagggtt actggacagc gaccccaatc
    12541 cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt tccgcatttg
    12601 ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgca tgaaccgccc
    12661 gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct
    12721 aaccgcccta acggtacggc gcataaatac tttgccagcg caccgtacaa aattgcggcg
    12781 aaatctggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa
    12841 attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg
    12901 caagtggctg tcgccatgat tctggagaac ggcggcgcgg gtccggcggt tggtacgctg
    12961 atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa
    13021 aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg aataaaaaaa
    13081 cattctggga taaagtccat ctcgatccca caatgctgct gattttactg gcattgctgg
    13141 tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgca
    13201 aaatcggcca aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa attcctccac
    13261 gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg
    13321 tagatgcttt cggtgccatc tctaaaggtg ctcaacgctg gctggacctc ggtattgttc
    13381 gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg cgctttatca
    13441 accgcgacgt ttgcccgcca tcgttgaaga acactggcat cgcgctggtg ctgatattta
    13501 tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt
    13561 ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag
    13621 tgctggtggc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc
    13681 agcgcgtaat gatgctcctg gacccggaat cagacccact cggcgcgggc tatcacatta
    13741 ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca
    13801 ctcagtcaca gcttgaattt ctccccgaac gccataccga ctttatcttc gcggtactgg
    13861 cggaagagct gggattagtg ggcattttga ttctgctcgc tctctacatt ctgctgatca
    13921 tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggtg
    13981 gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc
    14041 tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc
    14101 tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa
    14161 gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg
    14221 ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg
    14281 gtatgtaacg gccccatagt tgaaattagc ggggcggatc cgcgtttcga accactgaac
    14341 gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg
    14401 tctcgattta gccaggcggg actggcggca atctatgatg ccgaaccagg cagtaacctg
    14461 acggcctctg gcgaagcttt cgatccgaca cagctgacgg cggcccatcc aacgcttccg
    14521 ctccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt
    14581 aatgatcgcg gcccttacgg caacgaccgc gttatttcgc tttctcgcgc ggcagctgac
    14641 cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat
    14701 ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg
    14761 ccagcacctc ccgatttaag cggtggcgcg ggaacaagtt cagtgtctgg cccgcagggt
    14821 gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag atccgaccgg cgcgccggta
    14881 accagtagcg gtttcctcgg cgctccaacg accttagcgc ctggtgtact ggaaggcagc
    14941 gaaccgacgc ctgcgccaca gcccgttgtt acagctccgt cgacaacgcc tgcaacctcg
    15001 cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa
    15061 gtcggggccg ttagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag
    15121 ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcggatcca gcttggccca
    15181 ttcgccagca aagccgaagc cagcaccttg cagcaacgtt tgcaaaccga agcccaatta
    15241 cagtcattta ttaccaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt
    15301 aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata
    15361 gtagggcact ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt
    15421 ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc
    15481 tgctgcacat gccgatgacc tgaatatcaa aactatgatc ccgggtgtac cgcagatcga
    15541 tgcggagtcc tacatcctga ttgactataa ctccggcaaa gtgctcgccg aacagaacgc
    15601 agatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca
    15661 ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg
    15721 ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca
    15781 ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt
    15841 cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga tgaacagcta
    15901 cgttaacgca ctgggcctga aaaataccca cttccagacg gtacatggtc tggatgctga
    15961 tggtcagtac agctccgcgc gagatatggc gctgatcggc caggcattga tccgtgacgt
    16021 accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct
    16081 gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggcatca aaaccggaca
    16141 cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat
    16201 ttctgcggtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaactgct
    16261 aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc
    16321 ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt
    16381 gtacctgacc attccacgtg gtcgcatgaa agatctgaaa gccagctatg tgctgaacag
    16441 cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca acttccagct
    16501 tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg caagaaatcc cggaaggtaa
    16561 cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa
    16621 attgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaaactcc
    16681 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    16741 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    16801 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    16861 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    16921 actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc
    16981 atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc
    17041 gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca
    17101 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    17161 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    17221 ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga
    17281 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cgggacaaca
    17341 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    17401 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    17461 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgaat
    17521 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    17581 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    17641 acccgaagcg acgactaata atattgttcc acgtttactg gaaaatattt tagcgctact
    17701 aaacaatccg gacttcgaat atattaccgc ttaattccat acctcaatgg cccaatttac
    17761 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt
    17821 tctttatcac ttgcagaaat gcggtgccac gccttgctcc gtaactcacc ccattgatta
    17881 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat
    17941 tattgtaatt ttttaaatat tatttttcca tgaataaaat tggagtaagt gcgtggatag
    18001 taataatcaa attgaaccct gtttaagcag aaagtcgtca gaaggtaaac cacaaatatt
    18061 tacaacctta cgaaatattg atcttaacct tctgactatt tttgaagctg tatatgtaca
    18121 taaagggatc gttaatgcag cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc
    18181 tattcagaaa ctgcgcgtta tattccctga cccattgttt attcgcaaag gccagggtgt
    18241 cactcctacc gcatttgcga tgcatctaca tgagtatatc agtcagggcc ttgagtccat
    18301 tcttggcgcg ctggatatcg aaggaagcta tgataagcaa cgaacgataa ctattgcgac
    18361 cactccctcg gtcggagccc tggtccttcc tgtcatctat cgggcgatta aaactcacta
    18421 tccgcagctt ttactgcgca acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt
    18481 tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt
    18541 tctgttcacc gacaatatgg tgttaatttg ccgcgaggga aatccactac tctctttaga
    18601 agatgacaga gagactatcg acaacgctgc gcatgtactt ctgttaccgg aagagcaaaa
    18661 tttcagcggt ctgcggcaga gagttcaaga aatgtttccg gaccggcaaa tcaatctcac
    18721 cagctacaac attttgacaa tcgctgcact ggttgccaac agtgacatgt tagcgattat
    18781 tcccagccgt ttttataacc tgtttagccg ctgctggccg ctggaaaaac tgccttttcc
    18841 gtccttaaat gaggagcaaa tagatttctc tatccactac aacaaattca gcctgcgtga
    18901 tcccatcctg cacggagtaa tagatgttat ccgtaatgca ttttgaaaaa gtattgttac
    18961 agccagatca cagcgcagaa cgataaaatg gcacaatcca acaacaattt tacattttcg
    19021 cgaccgcttt ggctgctttc aggtccgttt caatgatata ctgccagtcg ttaattcaaa
    19081 aatagttgat aattacaaca atctattgaa ttgaaacgct ttccttcgta attcgcaact
    19141 ggaacacgca cgctatgagt aaacccattg tgatggaacg cggtgttaaa taccgcgatg
    19201 ccgataagat ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc
    19261 gcaagccgga atggatgaaa atcaagcttc cagcggactc tacacgtatc cagggcatca
    19321 aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc
    19381 tggcggaatg cttcaaccac ggcacagcaa cgtttatgat cctcggcgct atttgtaccc
    19441 gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt agctcctgat gccaatgaac
    19501 cagtgaaact ggcgcagacc attgccgata tggcgctgcg ttatgtggtt atcacctccg
    19561 ttgaccgtga tgacctgcgc gatggcggtg cccagcactt tgcggattgc attactgcca
    19621 ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt gccggatttc cgtggtcgta
    19681 tggatcgtgc tctggatatt ctgactgcaa cgccaccaga tgtgttcaac cataacctgg
    19741 aaaacgtacc gcgtatttac cgtcaggtac ggcctggtgc agattacaac tggtcgctga
    19801 agctgctgga acgctttaaa gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg
    19861 tgggactggg tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg
    19921 gtgtgacgat gttaacgctg gggcaatatt tgcagccaag ccgccatcac ctgccggttc
    19981 aacgttacgt tagcccggat gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct
    20041 ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga
    20101 aagggatgga agttaagtaa tcctgtaata attacttaca tctgtctgat aaaaaacccg
    20161 ctttttgaag cgggtttttt gtatcgaaca agatattgag ggagcgtcct gctcgccacg
    20221 tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc
    20281 agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc
    20341 cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa ctactagcag
    20401 tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc aacagaacgc
    20461 gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata gcaatctgta
    20521 acaaggtgaa tcaagcgccg agtaaaaaaa catcataata attgcggcgg cgcaaagcga
    20581 cggttgttta agacgggcag ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc
    20641 gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa gggatctata
    20701 atccggcttt gaccgatatt tttgttcccg cactcccccg ccgccaccat ataacaggtg
    20761 gtatccagcg cacgagcggc aagcaacgtt gaccaatgat gctctttgag cggcccgcga
    20821 acccaggcgg caggaagtac caggatttca gctccctgta atgcctgtgc cagcgccagc
    20881 tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccctc cacctcgagt
    20941 aacggagcga tttcattacc agcatcaaca cggcgtgatt cctgaatggc aaatgcatca
    21001 tagagatgca atttggcata acgggcgacg atgtttcctg cctgtagcgc caccagcata
    21061 ttccatgcgc gccccggcgt tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta
    21121 cgtttacttt ctcgccgtaa acgtccgagg agttcgcctt ccagcagctg tgctgattta
    21181 accgatagat ctgcatcatg atcatcgcgc gccagcaatg cttccggcag gacgaacagc
    21241 gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac aaatctcagc gttcttttcc
    21301 cacacagatg taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc ctgtcagagt
    21361 cgtaaaattc gccaacgacg attattcggc gttacactta tcactcatac aaatcaaata
    21421 gcaggatttt gcagtgttac aacttctttt agcagttttt attggcggtg gtacgggaag
    21481 cgtggcgaga tggctgttaa gtatgcgatt taacccgctg catcaggcga ttccgttggg
    21541 gacgctggca gcaaacctga ttggggcatt catcatagga atgggattcg cctggttcag
    21601 caggatgacg aacattgatc cagtgtggaa agtattaatc accaccggat tttgtggcgg
    21661 tctaacaacc ttctcaacat tttcggcaga agtggtgttt ttgttacaag agggccgctt
    21721 tggctgggcg ttactgaacg ttttcgtcaa ccttctgggg tcttttgcca tgacagcact
    21781 ggctttctgg ctgttttcgg cctcaaccgc acactaaaga ccaaaaaaaa acccgctgat
    21841 taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag
    21901 ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa
    21961 aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag
    22021 taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca
    22081 tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttacc agtacaccaa
    22141 ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt
    22201 gatgttactt attttagcta ttgattttaa agaagttact aaaacttcat ttgtctcaaa
    22261 actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg
    22321 tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag
    22381 ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa
    22441 aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc
    22501 atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat
    22561 cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt
    22621 ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa tcataatgtt
    22681 caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatccac tcatctgcgt
    22741 tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag atagcgacat
    22801 atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc
    22861 aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatct
//
LOCUS       DAZFYC010000075        22209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFYC010000075 DAZFYC010000000
VERSION     DAZFYC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.075"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(310..585)
                     /locus_tag="ACHKW5_003232"
     CDS             complement(310..585)
                     /locus_tag="ACHKW5_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO7512574.1"
                     /translation="MVNMRVFKIKCPECGSPAIIRKSDWKDKKLASLYCGCTEVECGH
                     TFVYSVEFSHTLSPSGLTGNKLVKFLIDRLKPEERQFALDLLSGQTT"
     gene            complement(633..1694)
                     /locus_tag="ACHKW5_003233"
     CDS             complement(633..1694)
                     /locus_tag="ACHKW5_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7512575.1"
                     /translation="MTKKQRNNKKFRSMTGSKVESFTPGRGSVITFGEPEPILTTGTD
                     YHNIWYDNEYDHWRLPIDRLALAQLPNLNGQHGGVLYARRNMVAGGYIGGGLTPDQVE
                     QAVFDYLLFGDVAILKIRNVFGEVIDLLPLPSLYLRCRKDGAFAVLQEGPALIYEPED
                     IVFFKMYDPRQQVYGLPDYIGGIHSVLLNSEATIFRRRYYNNGAHMGFILYTSDPNLT
                     LEMENEIKEKIAQSKGLGNFRNMFINIPKGDPEGVKIMPVGEVSAKDEFANIKGITAQ
                     DIFTAHRFPAGLAGIIPTNGAVMGNPETARTTYRKDEVIPLQRKLMNGVNNDPEIPAH
                     LHLVFDVDIPAIATEKGEK"
     gene            complement(1691..3502)
                     /locus_tag="ACHKW5_003234"
     CDS             complement(1691..3502)
                     /locus_tag="ACHKW5_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO7512576.1"
                     /translation="MAKYSDELKEAARTLYMKSWTPKDIAQELNIPPRTIYHWADVGK
                     WASLLPVESVENVIARRIDQLSRREKKTALELEELRDLIAHHVKLMAQRNKHAEKLAE
                     IQAQKAAYDGEGYCLSSAGGEPEERKRRYKKNDVSGITPEMLDTWAREHLFEYQLHCR
                     EHKGEDWRFILKSRQVGMTYYFAWEAFEDAVITGDNQVFFSASRSQSEIFREYIVQIA
                     QNHFGVTLTGKNIRLSNGAILRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASA
                     MATHNKYRTTYFSTPSAKTHQAYPVWTGEAWRGDDPKRKGVAFPTESEMRQGILCPDQ
                     IWRYIITMEDAIEGGLGALVDIERLRNKYSPTAFAMLYMCQFVDSKDAVFKFSALVGC
                     EVDRATWGDFDLTAARPFGNREVWAGFDPSRSGDNSTFVLIAPPIEDGERFRVLAVWQ
                     WQGFNFSWQADQIKQLMRRFNITYIGIDTTGIGKGVYDLVSKFAPREATPILYSVESK
                     NRLVMKMIDVVERKRIEWAKDAVDETNKERVEIPASFMAIRRTTTNSGNALTFVAERS
                     DATGHADVFFAISHAVINEPIDHEYDRPSGWYFGKAA"
     gene            3679..4740
                     /locus_tag="ACHKW5_003235"
     CDS             3679..4740
                     /locus_tag="ACHKW5_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7512577.1"
                     /translation="MPQSHYRTDWLCIATSGKAVDGRTIEPQWLIDAAETYTRKTYTA
                     MIWPHHPQCDISEREFTCNLGEVDALKVETEGDVTKLYAQLIPNQFLIDANRQGQKLF
                     TSAEFVTDFAGSGREYLFGLAVTDIPASLGTEKLKFVLAGEEKDAERGSLETFSLGKL
                     QTSKPDKKNSFWSRLFSASKDFTPTPEPNTDKPTEGDGEKMEELKALIQQMLDLLKSG
                     KDAATGDADTVDTPEQAADVVADVAAQIADAADEVAELAQDVIENPEDEVKAEEFSAA
                     KANLAKVMKSFNVTPAKRPRASRRRDFSARRQPAGNQMDNLTTQLTTVLTKLSAMENG
                     NTRRPGSAPGGSKEPFEFV"
     gene            4769..5791
                     /locus_tag="ACHKW5_003236"
     CDS             4769..5791
                     /locus_tag="ACHKW5_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7512578.1"
                     /translation="MQLTPKAEQMLRKFAAGLAKANGQVDTSRYFSLTNPKETQLRNA
                     LLQSSEFLRLLPNVLDVDQITGQVVSTGKPGIYTGRKKDGRFSRALGVSGNEYKLVET
                     DSGSYLPYSLLVVWANAGSEEEFFQRIQAFSNESFALDMLRVAFNGTSVAEDTDPEAN
                     PNGEDVNIGWHKIVKDRSSEQIISDAVTIGASGADFIGLDAAVTDLVHTCIYEPFRND
                     PRLVVLASADLIGNDATTMMNKIDRPTEKVAAQLIGRQIAGRTVYTPPFMPEGRLIVT
                     TLDNLHIYTQQGTRKRKAEWNDDRKRFENNYLRMEGYGVEHDELYAAFDKITLATGEV
                     APGGGA"
     gene            5794..6495
                     /gene="gpM"
                     /locus_tag="ACHKW5_003237"
     CDS             5794..6495
                     /gene="gpM"
                     /locus_tag="ACHKW5_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO7512579.1"
                     /translation="MAMTPCQRHRARVKATKALDKCEALTASPVSFHIQMLELERDVE
                     RLRSLTRAERMDMKRDVLLPRWMPTVEAYLAGDARFANPALVYCVIWLFDTGEMGKAL
                     DWADVAISESQATPENFKSNLPAFVADTVLEWAIMQAEAGHSIEPYFSRTFENIREKW
                     RLHEDINAKWFKFAGLYLLRDEKGQPRATAVDDVNTLEQADALLAQAAAYNKNAGVKT
                     MREKIRARINGLTQL"
     gene            6598..7071
                     /locus_tag="ACHKW5_003238"
     CDS             6598..7071
                     /locus_tag="ACHKW5_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006624209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7512580.1"
                     /translation="MSGPSFSISGKPVTVTPTAITNGVTFWPDLDLAEFQKVRTLPAD
                     LPPETAGVALLAAIAEVNDALADVVTYWNAKACERAADVPGAKMGDETQLTAQYKKAV
                     YARAKADLLGEFATIGRRESHPGQESQDTRASLLAEAANVMRNMLRQPRVGVHLI"
     gene            7068..7544
                     /locus_tag="ACHKW5_003239"
     CDS             7068..7544
                     /locus_tag="ACHKW5_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7512581.1"
                     /translation="MSQLESLTAFITANLPPDAMQMFSSSMEDCELVRNAKALGNNQR
                     RIGVLTYTARLSWDDFPYRKYSPGLIYALVLAWVDEFANELRDELKLADPTVDPEFDD
                     EGSCILDVVVPLVDPLVLREVEKGPIPFKGKKWDIVNPEIWEASQLEFIVQRGDAS"
     gene            7580..8236
                     /locus_tag="ACHKW5_003240"
     CDS             7580..8236
                     /locus_tag="ACHKW5_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512582.1"
                     /translation="MRETLAKADLPPRKRQRLLWRIAKLGIVAAAKRHQRQQAAPDGT
                     PWEPRKRGKGKMLKGLPKLLAVREMPEIQGVRIYLKGGNYRNGTKPIAAGLVGAVQQD
                     GARIQMKASNAPRKPQADKPALPRQAKRLRALGYKTRKGKRWVKPSSKQIMETMSMAQ
                     AGLLIRKLKGTPSKRTWTIDIPGRVFLGVSNDEFNQIIARQMQAIGFGWDVNAQQIRG
                     "
     gene            8239..9381
                     /locus_tag="ACHKW5_003241"
     CDS             8239..9381
                     /locus_tag="ACHKW5_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFO7512583.1"
                     /translation="MTWPNVNVSQKNRFNGTTNDVERVILFVGYGDTNIGKTQSLNTG
                     SDLDKALGDKDSPLKNMVAAAANNAGQNWFAYVHVLAEPDKEAEGYKPDEDWMNAVKM
                     AQSVASVEGVFLVFDTDEVATINRATEMRITLQANYGRFIWFGLAIGGPKQGEAWADY
                     VTRLAQLQDGIASPGVQLVPRLWGNEPGVLAGRLCNRSVTVADSPARVATGAVTALGR
                     DKLPVDGTGAEIDLSVLQSLQVNRYSVPMWYHDYDGIYWADGRTLDVEGGDYQVIENV
                     RVVDKASRRVRLRAIPKIADRSLNSTPGSIAAHETYFGKPLREIAISTQINGVEFPGE
                     VKPPKDGDITITWTSSEAVQIYLVVRPYESAKEISVSIELDTSLES"
     gene            9385..9840
                     /locus_tag="ACHKW5_003242"
     CDS             9385..9840
                     /locus_tag="ACHKW5_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFO7512584.1"
                     /translation="MTERISGGSFDVNYDSIMIHVENATVTITDNSAVAQSRGIPNGH
                     TKGSVSADVEVEVDSQSFKKFTAVARAAGSWRAIPAKDFLFYANAGDDEEKIEVFGCV
                     PTLSDIVNINPNEASKTTKKIKFMVTSPDFVAIDGVPYLSARDTRDLKG"
     gene            9845..10156
                     /locus_tag="ACHKW5_003243"
     CDS             9845..10156
                     /locus_tag="ACHKW5_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO7512585.1"
                     /translation="MMNGETSLLEKLLLIGAVIGLGQLMVSNERITTRLLVGRMILGS
                     AVAPLAAIPLLKFPDMPELVVIGLACALGILGSAFIEAGLKRCLDMYIKRWGSKRNET
                     E"
     gene            10143..10481
                     /locus_tag="ACHKW5_003244"
     CDS             10143..10481
                     /locus_tag="ACHKW5_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO7512586.1"
                     /translation="MKLSEKQQLFTVMIASLIHFAQEKGYRLTFGEAYRTPEQAALNA
                     KKGSGITNSLHTQRLAVDFNLFINGEYQTDSAAYRPLGEYWESIGGSWGGRFSKPDGN
                     HFSLEHNGVR"
     gene            10481..10864
                     /locus_tag="ACHKW5_003245"
     CDS             10481..10864
                     /locus_tag="ACHKW5_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512587.1"
                     /translation="MRNLLGLLLILAAAMSAGWQAHDWHDAKLKLAASEAAEQTRQIV
                     VEVTQQSGEALEAKLAELRANEIHTERVIRTETIKSVFSNVCASDDYVRLFNESADQA
                     ERKLSGKPAGTLPGHVATSGRTDRK"
     gene            10967..11236
                     /locus_tag="ACHKW5_003246"
     CDS             10967..11236
                     /locus_tag="ACHKW5_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFO7512588.1"
                     /translation="MSKSKKIAMTVAGVNLSFEPNKTAFNNLLNEMTMTNKVAPMVTY
                     LGRIVDAECKEALNKLMEDYPGCEMQIVEKVNEIYSPKLEIEVKN"
     gene            11287..11424
                     /locus_tag="ACHKW5_003247"
     CDS             11287..11424
                     /locus_tag="ACHKW5_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512589.1"
                     /translation="MRRYYLPHEDDDEESIARALWLDEYFAQTRASKTAEGIAIAFNG
                     N"
     gene            11426..13540
                     /locus_tag="ACHKW5_003248"
     CDS             11426..13540
                     /locus_tag="ACHKW5_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7512590.1"
                     /translation="MSHLDFTLSLIDKLTRPLKTAQSSLSGFAEKSQESFTKIGIGAA
                     AVWGVAQSIAGVVGPAYEMNAALAEVGSKGVAEDALKRMSGEAMRFSMRYGKGAVDVV
                     RSSYAMKGAMAGLSDLDLPRVTIAANTLAAGVKASGEEAGEYIGAMASRFNAELSSLG
                     HVRFAEELAGKTAYMVQNFGVKMQTMQELIEGTKSAGADFGVSLDEQFAVLGTLSRTL
                     GTEASGIYEQFLRSAPAAAEKLGMSFVDATGKMLPMGDILQKLQNKYGQSIEGNVKAQ
                     QALDAAFGGGADVIKKLYGQQDKLNRSITELGRNDGMKRAQEMAERMAEPWERIKATF
                     FAIRVAIGNTLFPILSPLMNRIADVGTKFARWLDMFPNIARWLGYITLGVLSFGLAGA
                     AVNIVMGVFGFTMTGLAAIAKVLGGAWKLLLWTLNLLRPSLLTTRIGLAALWIQSKLL
                     ALWTGVCRIALAAWNIALKAGAIAMRVYGAATMFAGAAMQLLMSPITLIVAGLALLAV
                     GVWYVITHWEELAAAIMDTSAFAWVMSVAEQVGQVFAQVWQSITDGWAVVVDFFAGLS
                     PLATFEGFAQTIGGVFSKLFDVLKNTFASTYNWIVEKLNKIPGVNIDLKTVSPPAAAA
                     VPANAVIPDSAAGSSKLNSPSVLTGNRINAGIPRGGLMSQVKNDSKTAVDNRKTWGDT
                     YINAPNGITPAQLAEWQELNAG"
     gene            13537..13872
                     /locus_tag="ACHKW5_003249"
     CDS             13537..13872
                     /locus_tag="ACHKW5_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006624195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFO7512591.1"
                     /translation="MSTEPLYIDLLITDGDFTLDSGNEPRRCDNRDSITQDIIHSILE
                     SGITTRLIGERSPTMRGDVLTQLSLLVESDERLVPGTIVITEETLSRLYITAETYDFG
                     PVSTEVNYD"
     gene            13865..15049
                     /locus_tag="ACHKW5_003250"
     CDS             13865..15049
                     /locus_tag="ACHKW5_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7512592.1"
                     /translation="MTEKPDVDFEKVLNDSGMPATEAEITAAFKATVQAEGFVTNTSR
                     MSPFWRLISKIVTTPVLWLRAALIDVVLRNMFVATATGPMLRLLAWAVHIEPKPASAA
                     AGVLRFFKLNAADVVVVPAGTLVQTERINGVVYVLAVNEDVTLPAGVESGLVPVTATG
                     TGSGYNLAPGYYRILPVAVAGIASAVNEDEWLITPGANEESDDELRDRTRNQFNLVGN
                     YHSDAIYRSMIASVLGLSIDRIYFLHDAPRGPGTANAYLLLDSGEISQPFIDAVNDYV
                     NTQGHHGHGDDLQCYAMPETSHTLAVTVYVKSVENMEAEDLSALKTGITDLIRCAFRE
                     NANYDVKKTQPYSRYSFSNLGREIHKAFPVVDSLHFSLTDIVSELSVPRLSGLTVEIE
                     ND"
     gene            15042..15635
                     /locus_tag="ACHKW5_003251"
     CDS             15042..15635
                     /locus_tag="ACHKW5_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7512593.1"
                     /translation="MTEFSKLLSGLKLPSWLNKGDPARLLRGSVKFWSQVYGWITWPL
                     KQFDPLVCPEPLLNLIAWERDIDRFKGEPLDIFRKRVSYAFINAQQAGEVAGFIAIFE
                     RLGIGYVELLERQDGLDWDVIVVRVTSSQVAENGDLLLEIIRKYGRTCRRYQFEVIDT
                     LPLNINIGWYQGEYICWPATLGDVNNQSEATYSASLK"
     gene            15647..17611
                     /locus_tag="ACHKW5_003252"
     CDS             15647..17611
                     /locus_tag="ACHKW5_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7512594.1"
                     /translation="MSQAVITKAFAEWKAQQAINNQPVTLDEFIFAYIPGLDADKPIN
                     NTETTPAEDKIVCRQAVNKAGVVNENSVVYSVTLGADVGDFDFNWIGLANKATGTLAM
                     IIHAPTQRKIKNADGQQGNVLVRSMLMEYSGAREATEINTPAETWQIDFTARLVGMDE
                     RQRRENIDLYGAAAFFDSGYLVAKSGNQFFVTKGAGYVAGLRAELPANFNITVSAKPT
                     KIWLDVSWTGTLTSEWAVQSKITVAADLADYVLGGVQHYVFAVASIDAAGNITDLRPK
                     GTLNDQAASDALKKHEQSRNHPDASTSEKGFVRLSSETNSDSEAMAVTPKALKAVNEN
                     ANGRVPASRKVNGHALNGDINVTSRDIFDGQVIAIGANKNLDDYQVPGLYFQEANNNT
                     SAAMNYPENSAGSLMVLRGAGVTQVYRVYNSSRSYSRSKYSTLAWTPWMPEDSYPVGA
                     PIPWPSDVTPTGYALMQGQPFDKAVYPLLAIAYPAGIIPDMRGQTIKGKPNGRAVLSY
                     EQDGVISHTHGASISDTDLGTKYTSSFDYGSKPTTSFDYGNKSSTEGGWHAHNFRYCA
                     TSAYRDTPGQGLGMHSSNVSWAAGDRIEGSGNHAHVTWIGPHDHWVGIGAHNHYVVMG
                     YHGHTATVHAAGNAENTVKNIAFNYIVRLA"
     gene            17611..18198
                     /locus_tag="ACHKW5_003253"
     CDS             17611..18198
                     /locus_tag="ACHKW5_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7512595.1"
                     /translation="MTFEMTGENRTITIYNLRADTNEFIGKSDGFIPANTGLPANSTN
                     IAPPPMKAGFVAVFNSASEKWSLVEDHRGKIVYDILTGKSITIDELGQLPDDVVSVAP
                     EGHFVKWNGKKWVHDADAEKTAQITQATQQKDSLLALAASKIAPLQDAVDLDIATEEE
                     TALLLAWKKYRVLINRIKPEDAPDIDWPEVPGDVA"
     gene            18188..18916
                     /locus_tag="ACHKW5_003254"
     CDS             18188..18916
                     /locus_tag="ACHKW5_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512596.1"
                     /translation="MWRESVIKIADDMGALACSIVPAHPWVYGLGQNTDSGGYLSPAN
                     ALGYLAKKLLSGGGSGSGDVIVMMVAENTHDAFMQGLNKLSTVFPAPVFTQVSRMAAA
                     AAELSTVKMQLPVKADVLPASAPLSVSTNRLALNAQRVAAAQLAAAVSTTTTDLKNQV
                     TGFIQERAGLLSSLSQGLDDLKAASANIFAFSYSGSYAVAAAELLKGIPQTTAVHTAA
                     MMFIGDSLSDLGKMLHEPDRITRA"
     gene            18888..19430
                     /locus_tag="ACHKW5_003255"
     CDS             18888..19430
                     /locus_tag="ACHKW5_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512597.1"
                     /translation="MSQTALLALDGEGIAMQNMLVSPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVSGLVTFDDEAVLQRLFQLASATEASGALKKYRVANATATAINLREATFT
                     GQIDAVPQEDRLAWQVSFTLREKGSVPEKRQARKGNATASTKQTGAKGAGPAAGADEP
                     ADKMSWFEEKVLKPVNDALG"
     gene            19438..21162
                     /locus_tag="ACHKW5_003256"
                     /pseudo
     CDS             19438..21162
                     /locus_tag="ACHKW5_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802618.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21747..>22209)
                     /locus_tag="ACHKW5_003257"
     CDS             complement(21747..>22209)
                     /locus_tag="ACHKW5_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFO7512598.1"
                     /translation="IHGKASSDQQLILGHGIDPINFEEKEAVPPENATDEELEEWRQC
                     MADNYDHSYEMGKQTINKYFTCSFKNTEKVIADSAKFFKNLSNVDEIIIIGHSLSSVD
                     VPYFSFIHSIVSENTEWIATYYQETEREAHHTTLCEIGIKSPKLISVSKLL"
BASE COUNT         5353 a         5630 c         6330 g         4896 t
ORIGIN      
        1 ttaatgataa aaaaccaatg gatgatattg gtgaactttc agattaaatg taatgtatca
       61 ttgtagagat ttattcttac agtgtttgaa cgcgtttatt cactaaggat tgtttctatg
      121 ttgtttatat aggatattga tagtgcgtaa aagtatgatt ttataatgtg taggcaatgg
      181 gctggatagg gcaatccttt acttatttga aaaccgccgc aatttgaatg tgcggcggag
      241 ggatgtaatg tacattaggt gttcaaagga ttgatgcttg cgccgtacat ctatgtgtag
      301 cagtgggatt tatgtagttt gcccactgag caggtccagc gcgaattgac gttcttccgg
      361 cttgagccgg tcaatcagga atttaaccag cttgttgccg gtcagcccgc tggggctgag
      421 ggtatgtgaa aactcaacgc tataaacaaa tgtatgacca cattccactt ctgtgcagcc
      481 acagtataaa gacgccagtt ttttatcttt ccagtcagat ttgcgaatga tggccggtga
      541 gccgcattca ggacatttaa ttttaaaaac tcgcatgttc accatcccgc acgccattgc
      601 taataatggg ggggatttta acttaactgc gctcattttt cgcccttttc ggtggcaatt
      661 gccgggatat ccacgtcaaa aaccaggtgc aagtgtgcgg ggatctccgg gtcattattg
      721 acgccgttca ttagcttgcg ctgcaagggg ataacttcat ccttgcgata ggttgtgcgg
      781 gcggtttcag ggttgcccat taccgcgccg ttggtcggga tgatacccgc caggccagcg
      841 gggaaacggt gggcggtaaa gatatcctgc gcggttatcc ctttgatgtt ggcaaattca
      901 tcctttgcgc tgacttcacc cactggcatg attttaaccc cttccgggtc gcccttcggg
      961 atgttgataa acatatttcg gaagttaccc agccctttgg actgggcgat cttctctttg
     1021 atttcatttt ccatttccag cgtaagattc ggatcgctgg tatagagaat aaagcccata
     1081 tgtgccccat tattgtagta acggcgtcgg aagatggttg cctcactgtt gagtaacacc
     1141 gaatggatgc cgccgatata gtcaggcagg ccatacacct gctggcgcgg gtcgtacatt
     1201 ttaaaaaaga cgatgtcttc cggttcatag ataagtgctg gcccttcctg caaaacagca
     1261 aacgccccgt cttttcggca gcgcagatac agcgacggca gcggcagcag gtcgatcact
     1321 tccccgaata cgttacgaat tttcaggata gcgacgtcac caaacagcag gtaatcaaag
     1381 acagcctgtt cgacctggtc aggcgtcagg ccgccgccga tgtagccacc ggcaaccata
     1441 ttgcgccgcg catacagtac cccaccgtgc tggccgttaa ggttcggcaa ctgggccagc
     1501 gccaggcgat caatcgggag tcgccagtgg tcatactcat tgtcatacca gatattgtga
     1561 taatcagtgc cggtcgtcag gatgggttcg ggttcgccaa atgtgatcac gcttccgcgc
     1621 cctggcgtga aggattcaac cttgctgccg gtcatggacc tgaatttttt attattacgc
     1681 tgtttctttg tcatgctgct ttcccaaagt accagcccga tgggcggtca tattcgtgat
     1741 cgataggttc gttaattacg gcgtgcgaga tagcgaagaa aacatctgca tggccggtcg
     1801 cgtctgaacg ttcggcaaca aacgtcagcg cgttgccgct gttggttgtc gtgcgccgga
     1861 tagccataaa gctggccgga atttcgacgc gctctttgtt cgtttcgtct acggcgtctt
     1921 ttgcccattc gatgcgctta cgctcgacaa cgtcgatcat cttcattacc agacggtttt
     1981 tgctttcgac gctgtaaaga atgggtgtgg cttcgcgcgg tgcaaacttg ctgaccaggt
     2041 cataaacgcc cttaccaatg ccggttgtat cgatcccgat gtaagtgatg ttaaagcggc
     2101 gcataagctg ctttatctgg tcggcctgcc agctgaagtt aaagccctgc cattgccaga
     2161 cggccagcac gcggaagcgc tcgccgtcct ctatgggggg cgcgattaac acaaaggtgg
     2221 agttgtcgcc ggatcgtgac gggtcaaagc ctgcccatac ttcgcgattg ccgaagggcc
     2281 gcgcagcggt cagatcaaag tcgccccatg tggcccggtc cacttcgcag ccgacaagcg
     2341 cggagaactt gaacaccgcg tccttactgt caacaaactg gcacatgtag agcatggcga
     2401 acgcggtcgg gctgtatttg ttgcgcagcc gttcaatatc gacaagcgca ccgaggccac
     2461 cttcgatagc gtcttccatc gttatgatgt agcgccagat ctggtccggg cagagaatac
     2521 cctggcgcat ttcgctttct gtcgggaacg ccacaccttt gcgtttcggg tcgtccccgc
     2581 gccaggcttc gccagtccat accgggtaag cctggtgcgt tttcgcgctg ggcgtcgaaa
     2641 agtaggtcgt ccggtatttg ttatgcgttg ccattgcgct ggcaacttcg tgcaggcgcg
     2701 tgaatttcgg gatccagaaa acctcatcgc catacaggtg gccgttaaag ccctgcgcgg
     2761 tgctggcgtt cgtggacaaa aagcgcagta ttgcgccgtt gctgaggcgg atatttttac
     2821 ccgtcagcgt cacgccgaaa tggttctggg cgatctggac gatgtattca cggaagattt
     2881 cagactggga gcggcttgca gagaaaaaga cctggttatc accggtaatg acagcatctt
     2941 cgaatgcctc ccaggcgaaa taataggtca tgccgacctg tcggcttttc agaatgaagc
     3001 gccagtcttc gcctttatgt tcgcggcagt gcagctggta ctcgaagaga tgttcccgcg
     3061 cccaggtgtc gagcatttcc ggtgtaatac cggaaacgtc gtttttctta taccggcgct
     3121 ttctttcctc tggttccccg cctgcgctgc tgaggcagta cccttcacca tcataagccg
     3181 ctttctgggc ctgaatttct gccagctttt cggcgtgttt gttgcgctgc gccatgagtt
     3241 tcacatggtg agcaatcaga tcgcgcagtt cttccagttc cagcgctgtt tttttctcgc
     3301 ggcgggagag ctggtcgata cggcgggcga tgacattttc caccgattca acaggcagca
     3361 gtgatgccca cttcccgaca tcagcccagt ggtaaattgt acgcggtgga atattcagtt
     3421 cctgcgcaat atctttcggc gtccagcttt tcatataaag cgtgcgggcc gcttctttta
     3481 attcatcgga atatttagcc atgcggctat tatggcggga ttattttgca gaattgataa
     3541 ttaaatatcg gtaaaaagtg gctatgtatt tataaccgaa tacataagaa ataaagcggt
     3601 cgcgctgttt taatcaattc gcaatactga ccaccacaaa cgaatcattg ttattaaatt
     3661 cattattaaa ggtcagttat gccgcaatct cattaccgca cggattggct atgtattgcc
     3721 acatctggaa aggctgtgga cggtcgcacc attgaaccgc aatggttgat tgatgcggca
     3781 gaaacctaca cccgcaaaac ctacacggcc atgatttggc cgcaccatcc gcaatgcgat
     3841 atcagcgaac gtgaatttac ctgcaatctg ggggaagtgg acgcgctgaa agtggaaacg
     3901 gaaggtgatg tcacgaagtt atatgcccag ttaatcccaa atcaattttt aattgatgcc
     3961 aatcggcagg ggcaaaagtt atttacgtca gcagaatttg tcactgattt tgcaggcagt
     4021 ggtcgtgaat atctttttgg gctggctgtg acggatattc cggcaagtct gggaacggaa
     4081 aaacttaaat tcgttttagc cggtgaagaa aaggacgccg aacgcgggag tctggaaaca
     4141 ttcagtttag gaaaattaca gacaagtaaa ccggataaaa aaaattcttt ctggtcgcgt
     4201 ttattttcgg ccagtaaaga ttttacgcca accccagagc caaacactga caagcccacc
     4261 gagggcgacg gagaaaaaat ggaagaatta aaagcgctca ttcagcaaat gctggatctg
     4321 cttaaaagcg gtaaagacgc cgcaacgggt gatgctgaca cggttgatac ccctgaacag
     4381 gccgctgatg tggtggcaga tgttgccgcc cagatcgctg atgctgccga cgaggtggcc
     4441 gagctggcgc aggacgttat cgaaaacccg gaagacgaag tcaaagcgga agaattcagc
     4501 gccgccaaag ccaacctggc aaaagtcatg aagtcattca atgtgacgcc agcgaagcgc
     4561 ccacgcgcca gccgtcgccg tgacttttcc gcccgtcgcc agccagctgg caaccagatg
     4621 gacaacctta ccacgcagct gactaccgtc ctgactaagt tgtcagcgat ggaaaacggc
     4681 aatacgcgcc gccctggcag tgcgccaggc ggaagcaaag aaccgtttga gtttgtttaa
     4741 cagccattct tttcaggaat aaaagattat gcaattaacc ccaaaagcag agcagatgct
     4801 gcgtaagttt gccgcaggcc tggcgaaagc taacggccag gtggacacgt cgcgctactt
     4861 ctcgctgaca aatccgaaag aaacccagct gcgcaatgcc ctgctgcaaa gttctgagtt
     4921 cctgcgcctg ctgcccaatg tgctggatgt ggaccagatc accggccagg tggtcagcac
     4981 cggtaaaccg ggtatttaca ccgggcgtaa aaaagatggt cgtttctccc gcgccctggg
     5041 cgtgtccggt aacgagtaca agctggtaga aacggattcc ggttcgtatc tgccttactc
     5101 cctgctggtt gtctgggcga acgcgggcag cgaagaggag ttcttccagc gtattcaggc
     5161 attcagcaac gaatctttcg cgctggatat gctgcgcgtg gcgttcaacg gaacgagtgt
     5221 tgcagaagat acagacccgg aagccaaccc gaatggcgaa gacgtcaaca tcggctggca
     5281 caaaatcgtt aaagaccgtt catctgagca gattatttct gatgccgtca ctatcggcgc
     5341 gtcgggtgct gactttattg gtctggatgc ggcagtcact gacctggtgc acacctgcat
     5401 ttatgagcca ttccgcaatg acccgcgcct ggttgtgctg gcttccgctg accttatcgg
     5461 caacgacgcc accaccatga tgaataagat tgatcgcccg actgagaaag tcgccgcgca
     5521 gcttattggc cgccagattg ctggccgtac cgtgtacacc ccgccgttta tgccggaggg
     5581 gcgccttatc gtcaccacgc tggacaacct gcatatctat acccagcagg gcacacgtaa
     5641 gcgtaaagcg gaatggaacg acgaccgcaa gcgcttcgag aataactatc tgcgcatgga
     5701 aggttacggc gtcgagcatg acgagctgta tgcggcattc gacaaaatca cccttgcgac
     5761 cggcgaagtt gcaccgggag ggggcgcgta aaaatggcca tgaccccgtg tcagcgacac
     5821 cgagcacgcg tgaaagccac aaaggcgctg gataagtgcg aagccctgac ggcatcgccg
     5881 gtcagctttc acattcaaat gctggagctg gaaagggatg ttgaacggct tcgcagtctg
     5941 acgcgtgcag aacgcatgga catgaagcgc gacgttcttt tgccgcgctg gatgccgacc
     6001 gtcgaggcat atctcgccgg tgatgcccgc tttgccaatc cggccctggt ttactgcgtg
     6061 atctggctgt tcgatacggg ggaaatgggc aaggcgctgg actgggctga cgtggctatc
     6121 agtgagagcc aggccacgcc ggaaaacttc aaaagcaacc tgcctgcctt tgtggccgac
     6181 acggtgctgg agtgggcgat catgcaggcg gaagccggtc acagcatcga accctatttc
     6241 agccgcacgt ttgaaaacat ccgcgaaaag tggcgtttgc acgaagacat taacgcgaag
     6301 tggttcaagt tcgccggtct ttacctgctg cgcgacgaga aggggcagcc gcgtgccact
     6361 gccgtggatg atgtgaacac gctggagcag gccgacgccc ttttggccca ggcggcggcg
     6421 tacaacaaaa acgccggagt taagaccatg cgcgaaaaaa tccgcgcccg gattaacggc
     6481 ctgacccagc tttaacgact cccgcaagcc gggacgggcg cgggggaggc atcaaccatg
     6541 cggttgttgg ccgtggaacc cgttagcccg tttctattgc aaaaccgagg tgcgccaatg
     6601 agtggcccaa gtttcagtat cagcggcaag ccggtgacgg tgacgccaac agcgatcacc
     6661 aacggcgtga cattctggcc cgatctcgat ctggccgagt ttcagaaggt gcgcacgcta
     6721 cccgccgatc tgccgccaga aaccgcaggc gtggccctgc tggctgctat tgcggaagta
     6781 aacgacgcgc tggccgacgt ggtgacgtac tggaacgcga aagcctgcga gcgggctgca
     6841 gatgtaccgg gtgcaaagat gggcgacgaa acccagttaa cagcccagta caaaaaagcg
     6901 gtctacgccc gcgcaaaggc cgatttgctg ggggagttcg ccaccatcgg gcggcgtgaa
     6961 tcgcatccgg ggcaggaaag ccaggacacc cgcgccagcc tgctggccga ggcggccaac
     7021 gtgatgcgaa acatgctgcg gcaaccacgc gtgggggtgc atctgatatg agccagcttg
     7081 aaagcctgac ggcgtttatt acggcaaatc tgccgcctga tgccatgcag atgttttcca
     7141 gttcaatgga ggattgcgag ctggtacgca acgccaaagc gctgggaaac aaccagcgcc
     7201 ggatcggagt gctgacttac accgcccgtt tgtcgtggga tgacttcccc taccgcaaat
     7261 attcgccggg gctgatttat gccctggtgc tggcctgggt ggatgagttc gccaacgagc
     7321 tgcgcgacga actgaagtta gccgatccca ccgtggaccc ggagttcgac gacgaagggt
     7381 catgcattct ggatgtggtt gtcccgctgg ttgatccgct ggtcctgcgt gaagtggaaa
     7441 aagggccgat ccctttcaag ggcaagaaat gggacatcgt aaaccccgaa atctgggagg
     7501 cgtcgcaact ggaatttatt gtccagcgtg gtgacgcgtc gtgatccgtg gggagctgaa
     7561 ccagcaacag ctaaagcaga tgcgggaaac gctggcaaaa gctgaccttc ccccgcgtaa
     7621 gcgccagcgc cttttatggc gtattgcaaa gctgggcatt gtcgcagcgg caaaacgtca
     7681 ccagcgccag caggcggccc cggacggtac gccgtgggag ccgcgcaagc gtggcaaagg
     7741 gaagatgtta aaagggctgc ccaagctgtt ggccgtgcgc gaaatgccgg agattcaggg
     7801 ggtaagaatt tacctcaagg gcgggaacta ccggaacggg acgaagccca tcgcggcggg
     7861 tctggtcggc gcggtccagc aggacggcgc acggatccag atgaaagcca gtaacgcccc
     7921 gcgtaagccg caggctgaca agccagcgct accgcgacag gccaagcgtc tgcgggcgct
     7981 gggttacaaa acccgcaagg gcaaacgctg ggttaagccg tccagcaagc aaatcatgga
     8041 aaccatgagc atggcccagg cgggattact gattcgaaaa ctgaaaggca caccctcaaa
     8101 acgcacatgg accattgata ttcctgggcg cgtttttctg ggggtgagca acgacgaatt
     8161 taaccaaatt attgcgcggc aaatgcaggc aatcggcttc ggctgggacg tcaacgcgca
     8221 gcaaatcagg gggtaaaaat gacctggccg aatgtcaacg tcagtcagaa aaaccgcttc
     8281 aacggcacaa cgaacgacgt cgagcgcgtc atcctctttg tgggttacgg cgacactaac
     8341 atcgggaaaa cccagtcgct gaataccggc agcgatctgg ataaagccct gggcgacaaa
     8401 gacagcccgt taaaaaatat ggtggccgca gcggccaata acgccggtca gaactggttt
     8461 gcttacgtgc atgtgctggc agaaccagac aaggaagccg agggctataa accagatgaa
     8521 gactggatga acgcggtcaa gatggcccag agcgtggcat ccgtggaagg ggttttcctg
     8581 gtatttgata ccgacgaagt ggcaaccatc aaccgtgcaa cggaaatgcg tatcaccttg
     8641 caggctaatt atgggcgatt tatctggttt ggccttgcga taggcgggcc gaaacagggc
     8701 gaagcatggg ccgattatgt gacgcgcctg gcacaactcc aggacggtat tgcgtcaccg
     8761 ggtgtgcaac tggtcccgcg tctgtggggc aatgaacccg gcgtcctggc cggtcgcttg
     8821 tgtaaccgtt cggtgacggt ggcagacagc cccgcccgcg ttgcaaccgg cgcagtcacc
     8881 gcgctggggc gtgacaaact gccggttgac gggacgggag ccgaaattga tctgtccgtg
     8941 ttgcagtccc tgcaggtgaa ccgctacagc gtgccgatgt ggtatcacga ctatgacggc
     9001 atctactggg ctgacggtcg caccctggac gttgaaggcg gcgattatca ggtcattgaa
     9061 aacgtgcgcg tggttgataa agcatcccga cgcgtccgtc tgcgtgctat ccccaaaatt
     9121 gccgatcgtt cactgaacag cacaccgggc agcattgccg cgcatgaaac ctatttcggc
     9181 aagccgctgc gtgaaatagc gatttcaacc cagatcaatg gcgtcgaatt tccgggcgaa
     9241 gtgaagccac caaaggacgg tgacatcacc atcacctgga ccagtagcga agcggtacag
     9301 atttaccttg tggttcgacc gtatgagagc gcgaaagaaa tcagcgtcag catcgaactg
     9361 gacacctcac tggagagcta atcaatgact gaacgtatta gcggcggatc gttcgatgtg
     9421 aactacgaca gcatcatgat tcacgtcgaa aacgccaccg tcaccattac ggataacagc
     9481 gccgttgcgc agtcgcgtgg catcccgaac ggccacacga aagggtcagt ttcggcggat
     9541 gtggaagtcg aagtcgattc ccagagcttt aaaaagttta ccgccgttgc ccgtgccgca
     9601 ggttcctggc gagccattcc ggcaaaggac tttttgttct atgccaatgc gggggatgac
     9661 gaagaaaaaa tcgaggtgtt tggctgtgtt ccgacgctgt ccgacatcgt caacatcaac
     9721 cccaacgagg ccagcaaaac cacgaaaaaa attaaattca tggtgacaag cccggacttt
     9781 gtcgcgattg acggcgtgcc gtacctgtca gcccgtgaca ctcgagatct gaaaggctga
     9841 cacgatgatg aacggagaaa cgtcactgct tgaaaagctg ttgcttatcg gggccgtgat
     9901 cggcctgggg caactgatgg tcagcaatga gcgaatcaca acccgtctgc tggtcgggcg
     9961 gatgattctg ggatctgcag tcgcaccgct ggccgcaatc ccgctgctga aattccccga
    10021 tatgccggaa ctggtcgtca ttgggctggc ctgcgccctg ggcattctgg gaagtgcgtt
    10081 tattgaggcg gggctaaagc gctgcctgga catgtatatc aagcgatggg ggagcaagcg
    10141 caatgaaact gagtgaaaaa cagcaacttt ttacggtgat gatcgccagc ctgattcatt
    10201 ttgcccagga aaaaggctat cgcctgacgt ttggcgaagc gtaccgcacg ccggaacagg
    10261 ccgcgcttaa cgcaaaaaaa gggagcggca ttactaacag cctgcatacc cagcgccttg
    10321 cggtggattt taacctgttt attaacggcg aataccagac cgacagcgcc gcatatcgcc
    10381 ccctgggcga atactgggaa tctatcggcg gatcgtgggg tggacgtttc agtaagccgg
    10441 acgggaacca tttcagtctt gagcataacg gggttcgctg atgcgcaatt tgctgggtct
    10501 tttgctgatt ctggcagctg caatgtcagc gggctggcag gcgcatgact ggcacgacgc
    10561 aaagctgaaa ctcgctgcca gtgaagcggc agaacaaacg cgccagattg ttgttgaggt
    10621 gacacagcag tctggtgaag cgctggaagc aaaactcgcg gagctgaggg ccaatgaaat
    10681 tcatacggaa cgggttatcc gcacggaaac cattaaatcg gtttttagca atgtttgtgc
    10741 ttctgatgat tacgtccggt tgttcaacga aagtgcagat caagccgaac gaaaattatc
    10801 aggaaaacca gctggcactt tgcccggtca cgttgccacg tctggccgga ccgaccggaa
    10861 gtgactttga cgcggcgctg aaagcctacc ggctgatgta taccgactgc gccgcccgac
    10921 ataacgccct ggtgggcatc attcgacaac gtaaggaatt agcacaatga gcaaatcgaa
    10981 aaaaatcgcc atgaccgtgg cgggcgtaaa tctgagcttt gagccgaata aaaccgcgtt
    11041 caacaacctg cttaacgaaa tgaccatgac caataaagtt gccccaatgg tgacgtatct
    11101 gggccgcatt gttgatgccg agtgtaaaga ggcgctcaac aagctgatgg aagattatcc
    11161 gggctgcgaa atgcagatcg tcgagaaggt taacgaaatt tactccccaa aacttgagat
    11221 cgaagtaaaa aactgacggc gcgggtagcg gccattcgca cgaatgccct ggagcaatac
    11281 cttgccttgc gccgctatta cctcccgcac gaagacgacg acgaagaaag catcgcccgc
    11341 gccctgtggc tggatgaata tttcgcccag acccgcgcca gtaagacggc ggaagggata
    11401 gcaatcgcat ttaacggaaa ctgatatgag ccacctggat tttaccctga gcctgatcga
    11461 taagctgacg cggccattaa agacggccca gtcttcgctg tctggctttg ctgaaaaatc
    11521 gcaggagtct tttacaaaaa tcgggattgg tgcggcggct gtctggggcg tggcgcagtc
    11581 catcgcgggc gtggtgggtc cggcgtatga gatgaacgcc gcgcttgcag aagtgggttc
    11641 caaaggcgta gcagaggacg cgctgaagcg tatgtccggc gaagccatgc gattcagtat
    11701 gcgctacggc aaaggggccg ttgatgtggt ccggtcaagc tacgcgatga aaggcgcaat
    11761 ggcgggcctg tccgatctgg acctgccccg cgttaccatc gcggccaata ccctggccgc
    11821 aggcgtcaag gccagcggcg aagaggcagg cgaatacatc ggcgcgatgg cgtcacgttt
    11881 caacgcggaa ttgtccagtc tggggcatgt gcgttttgcc gaagagctgg caggaaaaac
    11941 ggcgtacatg gtgcaaaact tcggcgtgaa aatgcagacc atgcaggagc ttatcgaggg
    12001 gacgaaaagc gccggtgctg actttggcgt cagcctggat gaacagttcg ccgtactggg
    12061 tacgctttcg cgcacgctgg gtactgaggc cagcgggatc tacgagcagt ttttacgcag
    12121 cgccccggct gccgctgaaa agctgggtat gagctttgtc gatgccaccg gaaaaatgct
    12181 gccgatgggc gacattctgc aaaaactcca gaacaaatac gggcagagca ttgaagggaa
    12241 cgtcaaggca caacaggcgc tggatgccgc gttcggtggt ggtgctgacg ttatcaaaaa
    12301 gctgtacggc cagcaggata aattaaaccg cagcatcacc gagctgggcc gaaatgacgg
    12361 gatgaaacgc gcccaggaaa tggccgaacg aatggctgag ccgtgggaac gtatcaaagc
    12421 gacattcttt gccattcgcg tggcgattgg taacacgctg ttccctatcc tgtcgccgct
    12481 gatgaaccgt attgccgacg tggggacaaa atttgcccgc tggctggata tgttcccgaa
    12541 tattgcccgc tggctgggtt acatcaccct gggcgtgctg tccttcgggt tggctggggc
    12601 ggcggtcaat atcgtgatgg gggtctttgg cttcaccatg acagggctgg ccgcaattgc
    12661 aaaagtgctg ggcggggcat ggaaactcct gttatggacg ctcaacctgt tgcgtccgtc
    12721 cctgctgacg acgcgcatcg gtctggctgc attgtggatc cagtcaaaat tactggcgct
    12781 gtggacgggc gtctgccgca tcgcgcttgc tgcatggaat atcgcgttaa aggccggagc
    12841 cattgccatg cgggtttacg gtgcggcgac catgtttgcc ggggcggcaa tgcaactcct
    12901 gatgagtccg atcactctga tcgttgccgg gctggcactt ctggccgtgg gggtctggta
    12961 tgtcatcacc cactgggaag aactggcggc agcaatcatg gatacgtcgg cttttgcctg
    13021 ggtgatgtcg gtcgctgaac aggtaggcca agtgtttgcg caggtctggc agtccatcac
    13081 agacggctgg gccgtggtgg ttgatttctt tgccggtctt tccccgcttg ccacctttga
    13141 agggttcgca caaaccatcg gcggggtatt cagcaaactt tttgacgtcc tcaaaaatac
    13201 ctttgcgtct acctataact ggattgtcga gaagttaaac aagatccccg gcgtaaacat
    13261 cgacctgaaa accgtttcgc caccggcagc ggctgccgtt ccggcaaatg cggtcattcc
    13321 tgacagtgct gcaggttcat cgaagctaaa cagcccgtcc gtgctgacgg ggaaccgaat
    13381 taatgcaggc atcccacgcg gcggcctgat gagtcaggtt aaaaacgaca gcaaaaccgc
    13441 tgttgataac cgtaaaacgt ggggcgatac ctacatcaac gcccccaatg gaatcacccc
    13501 ggcccagctg gctgaatggc aggagcttaa cgcaggatga gtaccgaacc gttatacatc
    13561 gaccttttga tcactgacgg cgatttcacg ctggacagcg gcaacgagcc gcgccgttgc
    13621 gataaccgcg acagcatcac ccaggacatt attcacagca ttctggaaag cggtatcacc
    13681 acccgcctga tcggtgaacg cagcccgaca atgcgcggtg acgtgctgac gcaactgtcc
    13741 ttactggtgg aaagcgacga acgtctggtc ccaggaacca tagtgatcac cgaagaaacc
    13801 ctttcgaggt tgtatatcac ggcggaaacc tacgatttcg gccctgtcag tacagaggtt
    13861 aactatgact gagaaacccg acgttgattt cgaaaaggta ctgaatgaca gcgggatgcc
    13921 cgcgacagag gccgaaatta cggcggcatt taaagccacc gtgcaggcgg aagggttcgt
    13981 cacaaacacg tcgagaatgt cacctttctg gcggctgatt tcgaagattg tcaccacgcc
    14041 ggtgttatgg ctgcgtgcgg cgctgatcga tgtggttctg cgcaatatgt ttgttgcgac
    14101 tgccaccggt cccatgctgc gcctgctggc ctgggcggtt catatcgagc ctaaaccggc
    14161 cagtgctgcc gctggcgtgc tgcgattctt caagctgaat gcggcggatg tggtcgtcgt
    14221 gcctgccgga acactggtgc aaacagagcg cattaacggc gtggtttacg tgctggccgt
    14281 gaatgaagac gtgacactgc ctgccggggt tgaaagcggg ctggttcccg tcacggcgac
    14341 cggcaccggc agcggctata accttgcgcc aggctattac cggatcttac ctgttgcagt
    14401 agctggaatc gccagcgcgg tcaatgagga cgaatggctg attacgccag gggctaacga
    14461 ggaaagcgac gacgagctgc gcgaccgcac ccgcaaccag tttaatctgg tggggaatta
    14521 ccactctgac gctatctacc gcagcatgat tgccagcgtg ctgggcctga gcattgatcg
    14581 catctacttt ttgcacgatg ccccgcgtgg gccgggtacg gcaaatgctt atctgttact
    14641 ggacagcggc gaaatatcac agccctttat tgatgcggtt aacgattatg tgaataccca
    14701 gggccaccac ggacacggtg atgatctgca gtgttatgcc atgccggaaa ccagtcacac
    14761 cctggcggtt actgtctatg tcaaaagcgt ggaaaacatg gaagcggaag acctgagcgc
    14821 gttaaaaacc ggtattaccg acctgattcg ttgcgcgttt cgcgagaacg ccaattacga
    14881 cgttaaaaag acgcagccct attcgcgcta ttccttttcg aatctgggcc gcgagatcca
    14941 caaggctttt ccggttgtcg attcactgca tttttcacta acggatattg tcagcgaact
    15001 gtcggtcccg cgcctgtcag ggttaacggt ggagattgaa aatgactgag ttttcgaagt
    15061 tgctttccgg tctgaaattg ccgtcgtggc tgaacaaagg cgaccccgcc aggctgttgc
    15121 gtggcagcgt gaagttctgg tcgcaggtgt acgggtggat cacctggccg ttaaagcagt
    15181 ttgacccgct ggtttgccct gagccgctgt tgaacctgat tgcctgggag cgcgacatcg
    15241 atcggtttaa gggggagccg ctcgacatct tccgcaagcg ggtgagttac gcatttatca
    15301 atgcgcagca ggccggagag gtggcgggct ttattgccat ttttgagcga ctggggattg
    15361 gttacgttga attactggaa cggcaggacg gactcgactg ggacgtgatt gtcgttcgag
    15421 tgacaagcag ccaggttgcg gaaaatggcg atctcctgct ggaaatcatt cgcaaatatg
    15481 ggcgcacatg ccgccgttac cagtttgaag taatcgacac cctgccactg aatatcaata
    15541 ttggctggta tcagggggaa tatatttgct ggcctgccac cctgggcgat gtgaataacc
    15601 agtcagaagc aacatatagc gcaagtttga agtagaggga atattaatgt cacaggccgt
    15661 cattacaaaa gcatttgcag agtggaaagc ccagcaggca attaataacc agcccgtcac
    15721 gctggatgag tttatttttg cttatattcc ggggctggat gctgacaagc cgattaataa
    15781 tactgaaaca acgccagcgg aagataaaat agtttgtcgt caggcggtga ataaagccgg
    15841 tgttgtgaat gaaaactctg tcgtttattc cgttaccctg ggggcggatg tgggcgattt
    15901 tgatttcaac tggatcgggc tggcaaataa ggcaacgggt acactggcga tgattattca
    15961 cgccccgacc caacgaaaaa ttaaaaacgc agacggtcag cagggaaacg ttcttgttcg
    16021 ttccatgctg atggaataca gcggagccag ggaagcgacc gaaattaaca ctccggcaga
    16081 gacatggcag attgatttta ctgcccgact ggtgggaatg gatgaacgcc agcgccggga
    16141 aaatatcgat ctgtatggcg cagcggcatt ttttgattcg ggctatctgg tcgcaaagtc
    16201 cggcaatcag ttttttgtca caaagggggc gggatatgtc gccggattgc gtgccgaatt
    16261 gcccgcaaac ttcaatatca cagtatccgc gaaaccgaca aaaatctggc ttgatgtgag
    16321 ctggaccggg acgttaacaa gcgaatgggc tgtacagagc aaaattactg ttgctgcaga
    16381 tcttgccgat tatgtgctgg gcggtgtgca gcattatgtc tttgcggtgg cgagtattga
    16441 tgctgccgga aatatcactg acctgcgccc gaaaggcacg ctaaatgacc aggcggccag
    16501 cgatgcgctg aaaaagcatg aacaatcccg aaatcaccct gatgcatcaa ccagtgaaaa
    16561 gggttttgtg cggttaagca gtgaaacgaa cagtgattcc gaagcgatgg ccgtcacgcc
    16621 aaaagcatta aaagcggtga atgagaatgc aaatggccgc gttccggcat cacgaaaagt
    16681 gaacggccat gccctgaatg gagatatcaa tgtcacttca cgggatattt ttgacggcca
    16741 ggttatagcg attggtgcaa ataagaatct ggatgattac caggtaccgg ggctttattt
    16801 tcaggaagcg aacaacaata ccagtgcagc aatgaattac ccggagaata gcgcgggttc
    16861 tctgatggta ctgagaggtg ccggagtcac tcaggtttat cgtgtgtaca acagctcgcg
    16921 cagttattcg cgcagcaagt attcaacgct ggcatggacg ccgtggatgc cagaagattc
    16981 ttaccctgtc ggcgcaccta tcccctggcc atcggatgtt accccgacag ggtacgcctt
    17041 aatgcagggg cagccctttg ataaagcggt ctatccattg ctagcgattg cctatcctgc
    17101 ggggattatc ccggacatgc gaggccagac gattaagggt aaaccgaacg gtcgcgcggt
    17161 actctcgtat gaacaggatg gtgttatatc gcatacccac ggagccagta tttccgatac
    17221 cgatttgggg acgaaataca ccagctcttt tgattatggt tcaaaaccaa caaccagttt
    17281 tgactacggc aataaatcct ccactgaggg tgggtggcac gcacataact ttcgttattg
    17341 cgcaacgtct gcataccggg atacccccgg tcaggggctg gggatgcatt cgtctaatgt
    17401 ttcatgggcg gcgggagatc gcattgaggg aagcggtaat catgctcatg tgacatggat
    17461 cggccctcat gatcactggg tgggtattgg tgcgcataac cattatgtgg ttatgggcta
    17521 tcacggacat acagcgaccg ttcatgccgc aggaaatgcg gaaaataccg ttaaaaatat
    17581 tgcgtttaac tacattgtga ggcttgcctg atgacttttg aaatgaccgg agaaaaccgg
    17641 acaattacca tctataacct gcgtgctgat acaaatgaat ttatcgggaa aagtgatggg
    17701 tttatccctg ctaataccgg tttgcctgct aacagtacca atattgcgcc accgccgatg
    17761 aaagccggtt ttgtcgctgt atttaattct gcgtcagaaa aatggtcact tgttgaagac
    17821 catcgcggga aaattgttta cgacattctc accgggaaat ccatcacgat tgatgaatta
    17881 ggtcagttac ctgacgacgt tgtttccgtt gcgccggaag gccattttgt taaatggaat
    17941 ggtaaaaaat gggtgcatga tgctgacgca gaaaaaacgg cacagattac acaggctaca
    18001 cagcaaaaag acagtcttct ggcgctggct gcatcaaaaa ttgccccatt acaggatgct
    18061 gttgatctgg atattgcaac ggaagaggaa acagcgcttt tgctggcgtg gaaaaaatac
    18121 agggttttga ttaatcgtat taagccagaa gatgcgccag atattgactg gccggaggtt
    18181 ccgggcgatg tggcgtgagt cggttataaa gattgctgat gatatggggg cgctggcctg
    18241 ttcaattgtg ccagcgcatc cctgggttta cggtctggga cagaacaccg attcaggcgg
    18301 ttatctcagt ccggccaatg cgctgggata ccttgctaaa aagctgttat ccggtggcgg
    18361 tagcggtagc ggtgatgtca tcgtcatgat ggtggcggag aatacccacg atgcttttat
    18421 gcagggactg aataaactgt ccaccgtatt tccggccccg gtatttacgc aggtaagccg
    18481 tatggccgca gccgccgcag aactcagcac ggtaaaaatg cagttgccgg ttaaagccga
    18541 tgtattgcca gccagtgcgc cgttatcagt ctcaaccaac cggctggcgc tgaatgccca
    18601 gcgtgttgcc gccgcgcagc tggccgccgc agtcagtacc accacaacag atctaaaaaa
    18661 ccaggtgacg ggatttattc aggaacgggc cggtttgctg tcctcgctca gccagggact
    18721 ggacgacctg aaagccgcca gtgcgaatat ttttgcattc agttacagcg ggagttatgc
    18781 cgttgctgcc gcagaattgc tgaaaggcat cccgcaaaca acggcagtgc ataccgccgc
    18841 gatgatgttt atcggggatt cgttatctga cttagggaag atgctacatg agccagaccg
    18901 cattactcgc gcttgatggt gaagggatcg ccatgcagaa catgctggtt tcaccttcca
    18961 tgcagtttca ggaaaaggac cagtcgggcc agacatcgag cacggccaat gctgaacagg
    19021 gtatcaaggc caaagagctg cgcgtatcgg gtctggtgac attcgacgac gaagccgtct
    19081 tacagcggct tttccagctg gcatccgcga ccgaagccag cggcgcactg aaaaagtacc
    19141 gcgtcgccaa tgcgacggcg acggctatca accttcgtga agccacgttt accggtcaga
    19201 ttgatgccgt accgcaggag gatcgccttg cctggcaggt aagtttcacc ctgcgtgaaa
    19261 aaggcagcgt cccggaaaaa cgacaggccc gaaaaggcaa cgcgacggcc agcaccaagc
    19321 aaacaggggc aaagggcgcg ggtccggctg ccggagctga tgagccagcc gacaaaatga
    19381 gctggtttga agaaaaggtc ttaaagccgg tcaacgatgc gctggggtaa ttaaacgatg
    19441 aaaccaatta aacgcctgta cctttcctct gatccggtcc atctgattga ctgcaatatc
    19501 gtgctggagc tgaacgcgtg cggtcggggg tttattacgg cggggacaga gacagattac
    19561 accggcaaaa tggtgcgtat cgatgttggt tatgatggtc tggtcctgcg ctggtttacc
    19621 gggtacgtcg aacggtcaca gcctgctgat aatggaacat gccggttgtt cgtgcgtgag
    19681 ctggtcggca tctttgataa attgtggccg tgttctttcc agcatccaac gttgcgccag
    19741 attactgact ggataagtga gcaacgcggg ctgaccgtca caacgccggt cggcgctgct
    19801 tatgcagata aaccgatccc ccactttacg cacagcggca cgggctatca gctttttgcc
    19861 agtctgggcc gtgcattttc agtgacggat tatctttggt atcagttgcc ggacggggat
    19921 gtcttcgtcg gcgctgcgga gcatagtctt tttgcgggaa aaccagtgga gatcccgcac
    19981 gaatttagcc aggaatcggc aggcggaaat tcaatggttg tgccaatgat tcagagcctg
    20041 cgcccgggtg cggaggtaaa cggccagcgg ctgaaccagg tccggctaaa taacgatgac
    20101 atggcaatta cctggcagcc ccgcaacaaa gccaacggcc agccattgca aaaatcaccc
    20161 attcagcggc agattgaaaa cgcattcccg gagctggcaa gcggcctgca tcttccaaga
    20221 ttcgctaggg tggaagcgcc aagcgaggat gtttcagggg gggatattgc cgatccattc
    20281 cgcccacgct atgccgtgga tctctagctg cttgacgaag acggcaagcc agccgcaaat
    20341 acgccgatct attctgccgt tccactacct gtgccaatgg cgggcagcga gtcaggaatg
    20401 tttcagtttc cgccccctgg cacgctggtt gaagttgggt tcactgaggg gcggcaggat
    20461 aagccctttg tgcgtcagat tatggcggag ggtcataacc tgccagcggt taaacccggt
    20521 gagcagttgc agcaacagcg cgatggtgta tcgcagcgcg tgacggttgc cggagactgg
    20581 gaacgccaga cggatcagac aatccgcgaa aactccatga ctctcgaagt caccaccgac
    20641 gaagagatcc gcaaagtggt tgcccgtgaa actacggtcc aggcaacaga taaaacaacc
    20701 gtgcttggca cggccacact tctggccggg gcggtcgttc atatcagcga gggggattac
    20761 agcgtcggca catccggcaa cctgacagta acctgcagca aggacaattc cgtcagtgtt
    20821 ggccggaacg tgaaacggga tgtcgcgggc aatgttacag acgacgtgaa aggggatgtc
    20881 acgtcaaacg tcagcggcgc actgactgaa aaaatcagcg gcattcgccg aagtgtggcc
    20941 caggcgtaac aactgattgc cccggtggta aagctgggaa gcgaagagat taacgtcctg
    21001 acactgctca ccgacaccct ggacgtggtg agggagctgg cagagactgc cgcatcacat
    21061 actcacccca atacgggggc cagtgggcag gcggcgcagt tcaccgcaac ggccaataaa
    21121 accggcacat tgaaaagcaa atacggcccc ctgatagcct gacaaaaaag cggcataacc
    21181 gcacacctca ccagacacca cagaacgcgc cacagcaggc gcaaagatta aaggtcgcca
    21241 ccgtgcggcc tttctttcgt tcgttcaaca gcggcccaca gcgtaagcga cagcaggcaa
    21301 acggaagcgg atccaggacg gaaacggcgc tacaccgcac ccgcctgcgc agtttggatc
    21361 ataaaaattt tgcaaaagaa attttgtgca aagcaccccg ccagcctgcg ccgctgctgg
    21421 gcttttgcat cctgcgggcg ttgcactctg cgcaaagatt tgcagcactt tgcaaaagtc
    21481 atcgcgccgc cagctactaa ctgactggat aacatgatgt ttttaaaagg atcgttttgg
    21541 tttccgtgac gatcaaaatg attaggtggt atccgatggt tttcgggggc ggatatcgtg
    21601 aagccttgtg cggtgagggc tggcggctag tttgctttga tttgggtttt gcaaaatgat
    21661 gcacgaagtt ttgataaaag gatctctagc taagggggag gggaacgtat aaaaaccccc
    21721 gccctctggg tgatattttg ttctatttat agaagtttac ttactgaaat cagtttggga
    21781 gatttaatgc caatctcaca taatgttgta tgatgtgctt cacgctcagt ttcctgataa
    21841 taggtggcta tccattccgt attttcacta acaatgctat gtataaaaga aaagtaggga
    21901 acatctacag atgataacga atggccgatg atgatgattt cgtctacgtt gctgagattt
    21961 ttaaaaaatt tagcgctgtc agcaatgact ttttcagtat tcttaaaaga acaagtgaag
    22021 tatttattaa tagtctgctt tcccatttca taagagtgat cgtaattatc agccatacac
    22081 tgacgccact cttcaagttc ttcatcggtt gcattttcgg gcgggactgc ttccttttct
    22141 tcaaagttga tagggtcaat accatgacct aaaatcaact gttgatctga tgatgctttt
    22201 ccatggata
//
LOCUS       DAZFYC010000076        21554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFYC010000076 DAZFYC010000000
VERSION     DAZFYC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.076"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..368
                     /locus_tag="ACHKW5_003258"
     CDS             <1..368
                     /locus_tag="ACHKW5_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7512599.1"
                     /translation="KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPI
                     NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKN
                     CFAEQEILSEFPQPVSLAG"
     gene            complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHKW5_003259"
     CDS             complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHKW5_003259"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO7512600.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHKW5_003260"
     CDS             complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHKW5_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7512601.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3315..4073)
                     /gene="aroD"
                     /locus_tag="ACHKW5_003261"
     CDS             complement(3315..4073)
                     /gene="aroD"
                     /locus_tag="ACHKW5_003261"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO7512602.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4104..4970)
                     /gene="ydiB"
                     /locus_tag="ACHKW5_003262"
     CDS             complement(4104..4970)
                     /gene="ydiB"
                     /locus_tag="ACHKW5_003262"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO7512603.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4982..6247)
                     /gene="ydiN"
                     /locus_tag="ACHKW5_003263"
     CDS             complement(4982..6247)
                     /gene="ydiN"
                     /locus_tag="ACHKW5_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7512604.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6474..7688)
                     /gene="ydiM"
                     /locus_tag="ACHKW5_003264"
     CDS             complement(6474..7688)
                     /gene="ydiM"
                     /locus_tag="ACHKW5_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7512605.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFFSFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7788..8144)
                     /gene="ydiL"
                     /locus_tag="ACHKW5_003265"
     CDS             complement(7788..8144)
                     /gene="ydiL"
                     /locus_tag="ACHKW5_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO7512606.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8280..8387)
                     /gene="rprA"
                     /locus_tag="ACHKW5_003266"
     ncRNA           complement(8280..8387)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKW5_003266"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8573..9685)
                     /gene="ydiK"
                     /locus_tag="ACHKW5_003267"
     CDS             complement(8573..9685)
                     /gene="ydiK"
                     /locus_tag="ACHKW5_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO7512607.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10074..13130
                     /gene="ydiJ"
                     /locus_tag="ACHKW5_003268"
     CDS             10074..13130
                     /gene="ydiJ"
                     /locus_tag="ACHKW5_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO7512608.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIVSQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQISI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13127..13537
                     /gene="menI"
                     /locus_tag="ACHKW5_003269"
     CDS             13127..13537
                     /gene="menI"
                     /locus_tag="ACHKW5_003269"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO7512609.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13637..13825
                     /gene="ydiH"
                     /locus_tag="ACHKW5_003270"
     CDS             13637..13825
                     /gene="ydiH"
                     /locus_tag="ACHKW5_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO7512610.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14373..14741
                     /gene="sufA"
                     /locus_tag="ACHKW5_003271"
     CDS             14373..14741
                     /gene="sufA"
                     /locus_tag="ACHKW5_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO7512611.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14750..16237
                     /gene="sufB"
                     /locus_tag="ACHKW5_003272"
     CDS             14750..16237
                     /gene="sufB"
                     /locus_tag="ACHKW5_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO7512612.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16247..16993
                     /gene="sufC"
                     /locus_tag="ACHKW5_003273"
     CDS             16247..16993
                     /gene="sufC"
                     /locus_tag="ACHKW5_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO7512613.1"
                     /translation="MLSIKDLHVSVEGKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16968..18239
                     /gene="sufD"
                     /locus_tag="ACHKW5_003274"
     CDS             16968..18239
                     /gene="sufD"
                     /locus_tag="ACHKW5_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO7512614.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPFLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18236..19456
                     /gene="sufS"
                     /locus_tag="ACHKW5_003275"
     CDS             18236..19456
                     /gene="sufS"
                     /locus_tag="ACHKW5_003275"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO7512615.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19469..19885
                     /gene="sufE"
                     /locus_tag="ACHKW5_003276"
     CDS             19469..19885
                     /gene="sufE"
                     /locus_tag="ACHKW5_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO7512616.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20034..21038
                     /gene="ldtE"
                     /locus_tag="ACHKW5_003277"
     CDS             20034..21038
                     /gene="ldtE"
                     /locus_tag="ACHKW5_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO7512617.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21102..21338)
                     /gene="lpp"
                     /locus_tag="ACHKW5_003278"
     CDS             complement(21102..21338)
                     /gene="lpp"
                     /locus_tag="ACHKW5_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO7512618.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5622 a         5236 c         5441 g         5255 t
ORIGIN      
        1 taaaagatat tcttttctat ctgaataata attatcgcga aaaaatcact ttagaacagt
       61 tatcgaaaaa atttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc
      121 gtatttcccc tattaactat gttattcaac ggcgtatgac ggaagcgaaa tggtcactca
      181 ctaatactga attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
      241 actttgccaa actgtttttg cgccatgtcg gttgttcgcc cagcgattac cgcaggcaat
      301 ttaaaaactg ttttgcggaa caagaaatcc tatctgaatt tcctcaaccg gtaagtcttg
      361 ccgggtaaat ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc
      421 tgataatctt tcaggatctg ccgacccgca acgtaaatca taatttcgtc tgtaccgccg
      481 ccgatacgtt cacaacggac atcacgccag aagcgggaga cgcgcgcctc atcggtatag
      541 cccagaccgc ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcacag
      601 tacagttttg ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc
      661 actttcagca ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc
      721 tggatcatct ggttatgacc aatgggctta ccaaaagcga tacgttggtt ggcatagcgg
      781 gcggcatctt caaaggcgca ttcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc
      841 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
      901 ctctcttcaa cctccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
      961 attttatgca gcgggttaat cttaatgccg ggcttactgg agtcgaccca ccacagggtg
     1021 aaggcttttt tgggatcttt cggttgcgga tcgcgcgcca gcaccagcat atacgggtac
     1081 tctttcgcac cggtaataaa ggttttttgt ccgttgatgt agaccttgcc gtttttacgc
     1141 gtgtaagtgg tagtggcact gttgttatct gagccagcac ctggttccgt caacgccagg
     1201 gcataggcgg gatcacccgt ttccagggtg ctctctgccg ttttacgtag ctgctctgca
     1261 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg taattaaaaa tgccggagca
     1321 ccgcattttg acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca
     1381 ccaaattctt ccggcacgcc aagcatggaa ataccgttat ccgccagcgc ccgcataaac
     1441 tcacgcggat atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc
     1501 gtaatcagtt cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc
     1561 atcattatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
     1621 aaataatctt tcgtccatca gtttgagttc tggcgaaatc actggggtga agtccatttt
     1681 gtcgagaata tctttttgta aatcgacgcc aggggcgatt tcgattaaat gcaggccgtc
     1741 ttctttcagc gtgaatacag cacgttcggt gatgtaacga acatccagtc cccgttcgag
     1801 cgcgattttt ccgctaaaag taatttccgg tagttcccga ataaatttat ttacccgtcc
     1861 ttcctggacg atatgcaatt tgccatcggc aatttctgtt tttaaactgc ccgcagttaa
     1921 cgtgccgcag aaaataattt tcttcgaggt ggcactgata tcaataaatc caccggtacc
     1981 catgattttg ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
     2041 actcaaataa caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc
     2101 cagaatggcg cgggtattca cgttcgcgcc aaaggcgata ccctgtgaag taattccgcc
     2161 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac
     2221 caggccaata ccgtcagcaa taccgacgcc gacattcccc accgcgcctt tacgcatttc
     2281 gaataatgca cgccgcgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct
     2341 gtcatcaagg gtgaagtcac cagaaataaa acggttaacc ggcgcaccgc catacagttg
     2401 gctttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
     2461 aggatgcagc gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
     2521 attgtgcacc gcctgggcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt
     2581 ggcgtagcct tcactgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgcaatcgc
     2641 tttgtaatag agatattctt tgttatcaaa ctcgaccagt ttaatcaggt cttctttcgt
     2701 gacttcattc agtttgccgc cttgctggcg tggatcgaca aatgtcccga tgccaatatc
     2761 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
     2821 tggatagtta taagcaataa ttttattttg ttctgcgagt tcagaaatac gcggcgattg
     2881 tccccagtga ccacataatg cccatttcac cagaccttct tgcgccagag gactaatacc
     2941 acggtcggcg cgatcgccaa gccctgtcgg actaataatc gataaattac gtggcgtttg
     3001 agtctgttta tatttatcag caagagctgt aattaacgtg gtggcttcca gaataccgcc
     3061 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
     3121 cgacaggacc ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc
     3181 ctgattcaga tgtgattttc cctacgccag atggagaatt agcgcacaga gactcacgaa
     3241 atatttttat taaatacggt taaatatatt ttttaagttc ggcggggagg gtgttcccgc
     3301 cgaaatatta ttgcttatgc ctggtgtaaa atagttaata ccgtgcgtaa atcatttacc
     3361 gagatttgcc ctggtgcaga ggcttttttt accgcaccaa aagttgccgc cgagccaaat
     3421 acttcaccag ccagacgaga aattacgcca gtttttgcca tcgacatcgt gatgattgga
     3481 cgatcggcat actgctcctg catctccagg gtcgcggtaa gcaacgtcag cacatcgctg
     3541 gtactttgcg gcatcagcgc aatcttagga atatcggcgt cgaaggactg cattttgcgc
     3601 agacgggcaa tgatttcttc ggcttccggc gttttatgga agtcatggtt ggacatgaca
     3661 actttcacat catgcgcgtg ggcgtaggcg acggtttctt taacctgatc atcgccggta
     3721 aataactcca gatcgatcat atcaaccagg ccgctgtcga tggctgcacg attgagagcg
     3781 atataagcct cggtggaaat cgcctgctcg ccgccttctt tggcactgcg gaaggtaaac
     3841 agcagcggtt tttctggcat ggtttcacgg agaatttttg ccgccgccat gacagactcc
     3901 acattggaga ggtcggcaaa gtggtccaca cgccattcca gaatatcaaa gtccgcttca
     3961 cgataggcga gagcttcgga tttcacgcgg gcgatatctt tcgccatcag cgagacgatg
     4021 attttaggtg cgcccgtacc aatgacgaga tcttttacag ttacggtttt cattttttac
     4081 cctttctgca cgcggtcagc ctgtcaggca ccgaacccca tgacctgttt aacatattcc
     4141 agagggaaat ctttgccagt ccataacgtg aactgttcag ccccttgcca caacaacatg
     4201 ccgtatccat caatcgtttt gcaaccagct tgttgcgcct gctgcaataa cttcgtcata
     4261 tgcgggttat acacgcattc agtgaccagc agtcccggat gtaacagact gatatcatta
     4321 actaatgatt cattctcaag gggtttcata cccacttttg tgccattggt taaaatgtcg
     4381 gcggaagcca gggcttcagc aaaggcttgc tgatcggcga gatcggtaac cgtgacgaca
     4441 caatcggtgt tttcgttaac ccgctgcgcg aaggcgaggg ctttatcgaa gaactcatcc
     4501 cgacggttaa agagtttaat ttcttttaaa ccttcaattg ccccctgcgc gccaatcgcc
     4561 gttgaggcac ctccggcccc taacagcacc attgttttgc ctttgatatc aaaaccgctc
     4621 tctttaatgg cgcgaatatg gcccgtgccg tcggtgttat agccgcgcag gtagccatca
     4681 tcattaacga tggtgttgat ggcacccacc agtttggctg ctggcgttaa ttcatcaaca
     4741 tattcacacg ccagttgttt gttcggcatt gatataccag ttccgcgcat tttgagggct
     4801 tttaatcctt caattgctcc gggaaaacta tcgttatcca cttcaaaggc catataggta
     4861 aatggcaatc ccgctttttc taaggcttta ttctgcattt caggcgataa actgtggcgg
     4921 ataggatagg ccatcagccc aatcaattcg tattttgcgg taacatccat aattaactcc
     4981 tttaacctct atgcttaatt gtgtttaacc gggtgctaaa tttacgctcg ccaaaccgca
     5041 catcattctc aggaataata aaaacgcggt aatagcggat aaaaacaata attgcggtaa
     5101 tcagggccag cagcgcgaaa gtaaaatcga gaacaatgat atattgaagg ccgatgttcg
     5161 acagataacc ggtaatcagc ggaataacaa agttagccag tccacccatc atcatataaa
     5221 tactggtgac tttggctttg ctttttggga aaaactctga catgaccgaa acaccgagct
     5281 gtaaaatgcc gccagctgct gagaaaccga taacaaaggc gccggcatta cacaccagtg
     5341 gagaagggta caggtagata atggctgctg ttattgttgc cagtgcagag ttaaatacat
     5401 tagcccagat gggccggacc atttttttca gtagtgcggc aaaaataaag acacagacca
     5461 acgagcccat actgtaataa gagatggttt ttaatgcctc agcttctgac ataccagcaa
     5521 aagccatcgc atatttgggc atccacacca caatcacata aaaggtcgag aatgcggcta
     5581 caccgaacag taccgatgaa acaccttcca gccagactaa cggtttgctg ttcatttgcg
     5641 gtaattcatt agctacgctg gcgtccacca actggctggg gaatttgctt ttcaacagca
     5701 tcagcgtgat cagtacaaac agaatacccg gaataatcag cccatagccg taccagatat
     5761 tattgagcaa catatagctc accagcattg ggtagaacat ttgcccaaat gacaccatcg
     5821 ctttaaccag tatgaccgcc gaaccagagg ctttcggaaa gcattccatg agcgcggggt
     5881 agccacccgt atccagcgct gagttagcga tacctacgca cactgccaga ccgtaggcga
     5941 gagttaaatt cgggcaagcg ggaataccaa agaagaatag cagatacatt attactgcca
     6001 ttaatatcac cgcccgacga ccaaacttat cggagatcac accgaagaat aaaatactga
     6061 tcaatcgccc caaaccgata ccggaaatta agtaggcaat gcccgcgttg tcagtggaaa
     6121 acttttctgc cagagatgac atattttggg caagcgtaat aacactaatg ccgtgcagga
     6181 aatagctgaa gtaaatacaa agaacagcca ggataaatgg cgtgctgaaa gccttatttt
     6241 gagacatttt cacagctcct tgctgaaaga taatttcgtt accgtacgta ttgttctccg
     6301 acggtttaca taattactat actgaagatg attttccctg ggtacatggc ataaagttta
     6361 tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc ttagttagcg
     6421 cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt gcattaccca
     6481 cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct gcgtaagccg
     6541 ataaacagtg ccagtaaaaa accgatggca gcgatggcgg tatcgaacca cataatatcg
     6601 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     6661 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     6721 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     6781 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagaaa
     6841 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     6901 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttaat cgacatagtg
     6961 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     7021 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     7081 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     7141 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     7201 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     7261 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     7321 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     7381 aagccaaaaa cataggcgat aatgatgtta ttggtgtgca ggatgccaaa aaagaaggcc
     7441 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     7501 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     7561 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     7621 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     7681 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     7741 tccgttcatt attgttgtaa caacgacaat tcctttttgt ggagaaatta acaaagccgc
     7801 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     7861 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     7921 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     7981 cgcatagcca acagttgctc gactacacga tcaggaatgg cgcatttgcc attttcccac
     8041 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     8101 atggcaaaaa tatggcgaag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     8161 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     8221 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     8281 taaaaaaaag cccatcgtgg gagatgggca aagactacac acagcaattc gttgtttcac
     8341 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtaag ctaatagtag
     8401 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     8461 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagtaagcaa
     8521 atatcaatta gcgggggtta acaatcaggc ggcagagccg cctgatgaga aattacttat
     8581 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggcggcg
     8641 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     8701 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacaccagag aggatcagga
     8761 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     8821 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgcca gtccagtaga
     8881 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     8941 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     9001 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     9061 gtgccgccag cagtacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     9121 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     9181 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     9241 tggtgccaat ataagggcgg actttcgcca tgatcgccgt cccccccata tccagcaagt
     9301 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     9361 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     9421 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     9481 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     9541 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     9601 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     9661 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     9721 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     9781 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     9841 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     9901 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     9961 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
    10021 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
    10081 cacagatttc ccaggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
    10141 agcaacaagg ttttaccggc gatacggcga caagttatgc cgatcgtctg acaatgtcga
    10201 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
    10261 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
    10321 cccgtggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
    10381 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
    10441 aggccggggt gataaaagat caactcaatc agtacctgaa accgttcggc tacttttttg
    10501 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgctt
    10561 ctggtcaggg atcgctggtc tatggcaaaa cgtcggatca cgtacttggc gtacgtgcgg
    10621 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgaattg gcggaaacgc
    10681 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
    10741 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
    10801 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
    10861 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
    10921 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
    10981 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
    11041 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat gtgcctgaca
    11101 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
    11161 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgtcagccaa caggcaggtg
    11221 tgattggctg gcaggtgtgc cgcgagctgg cgggtgttga acgtatctat gcgatgcgca
    11281 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
    11341 aggatacctg tgtaccgccg gaacatctgg cggattatat tgctgaattt cgcgcgctgc
    11401 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gttttgcacg
    11461 tccgtccagc gctggatatg tgcgatcctc aacaagagat tttgatgaag caaatctctg
    11521 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
    11581 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
    11641 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
    11701 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
    11761 agatctccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
    11821 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
    11881 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgt ttgttggcgg
    11941 atcgcggcgt tgatccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
    12001 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
    12061 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcgtgt tcgacccagt
    12121 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
    12181 gttatttacg cccgctgcgc gaccacctcg tcgctacggt cgagagctac gcgccgctga
    12241 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
    12301 cggaaaaaca tatcggcatg gttgatttgc cgctgctgtc ggtcccctcg ctacaacaac
    12361 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaatct ctcaatgcag
    12421 agcagaaagc gcgcacagtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
    12481 aagtggtggc ggattttgtc cgtctggtcg aaaaattagg tttccagcct gtgttactgc
    12541 cattttcgcc aaatggcaaa gcccagcata ttaaaggttt tcttaatcgt tttgcgaaga
    12601 cggcgaaaaa gacggcggat ttcctcaacc gtatggcgaa gctgggtatg ccaatggtgg
    12661 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
    12721 aacgtggcga gtttaacgtc ttactggcga atgaatggct ggcaagcgca cttgagtcac
    12781 agccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaagtta
    12841 ccgccttgcc gggtgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
    12901 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
    12961 accataaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
    13021 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
    13081 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
    13141 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
    13201 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
    13261 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    13321 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    13381 caatgctaac cacgtccgct cggcacgaga agggcgggtg cgcggggtat gcaaaccgtt
    13441 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    13501 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    13561 gcatattgcc tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    13621 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    13681 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    13741 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    13801 tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    13861 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    13921 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    13981 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    14041 gggtagaaac ccttaagcct gtgttgcaca gacttaaggg ttttcttatt tctgaacgct
    14101 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    14161 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    14221 tgcctgttaa cccacacatc agggtctatg cttattaaaa gcaggcaaaa agcatgtttt
    14281 acggtaaagc ccctgcgttt gctgagttga actgataatc attatcacta acatgctgtt
    14341 atacgctgaa agcgatgaag tgaggtaaat cgatggatat gcattcagga acctttaacc
    14401 cacaagattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgtg
    14461 agctggtggc aaagcagccg ggtatggtcg gcgtgcgctt aggcgtgaag caaacgggct
    14521 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    14581 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaagc gatgccgttt attgatggca
    14641 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    14701 cccagaatga atgtggctgt ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa
    14761 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaatt ataaagaagg
    14821 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    14881 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    14941 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    15001 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    15061 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    15121 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    15181 cattttcgac tcagtttcgg ttgccactac ttatcgcgaa aaactggcgg agcagggaat
    15241 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    15301 cggcaccgtg gtgccgggga atgacaactt ctttgccgcg cttaatgcgg cggtagcctc
    15361 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    15421 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgt accattctgg tggccgacga
    15481 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    15541 acacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    15601 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    15661 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtccgc
    15721 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    15781 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    15841 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    15901 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    15961 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    16021 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    16081 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    16141 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    16201 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    16261 agatttacac gtcagcgtgg aaggtaaagc tatcctgcgt ggattaagcc tcgccgttcg
    16321 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    16381 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    16441 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    16501 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    16561 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    16621 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    16681 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    16741 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    16801 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    16861 acgcattctc gactacatca agcctgatta cgtccatgtg ctatatcagg gacgaattgt
    16921 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    16981 cgaacagcag taacgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    17041 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    17101 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagccag tttgtcagca
    17161 ttgcgggaga gatatcccca cagcagcgag atgccttagc gttaacgtta gacgctgtgc
    17221 ggctggtatt tgtcgatgga cgttacgtgt cagcactgag cgatgcgact gaaggcagcg
    17281 ggtatgaagt gagcattaac gacgaccgtc agggggtacc cgacgctatt caggcggaag
    17341 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    17401 gtcaacggcc ggcaaagcca tttctgttaa tgcatattac ccagggcgtg gcaggtgaag
    17461 aggtcaacac tgcccattac cgacatcatc tggatctggc ggaaggtgcc gaagcaacgg
    17521 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcactt caccggcgca cggttcacta
    17581 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    17641 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    17701 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    17761 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    17821 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    17881 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    17941 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggca gaagtggata
    18001 cgaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    18061 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    18121 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    18181 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacgact gccaggaggt gcaagatgac
    18241 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    18301 gccgctggct tatctcgaca gcgccgccag tgcgcagaaa ccaagccagg tgattgactc
    18361 cgaagccgag ttttatcgtc acggctacgc ggcggtgcat cgcggtattc ataccttaag
    18421 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    18481 ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc
    18541 caatagttgg ggcaacagca acgtgcgggc gggcgataac atcatcatca gtcagatgga
    18601 gcaccacgct aacattgttc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    18661 tgtgatcccg ctcaatcccg acggtacgct gcaactggag acgctaccta atctgttcga
    18721 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    18781 cctggcggaa atgatcacgc ttgcgcatca gcatggcgca aaagtgctgg tggatggcgc
    18841 tcaggcggtg atgcatcatc cggtggacgt tcaggcgctg gattgcgatt tttacgtatt
    18901 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    18961 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    19021 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    19081 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    19141 agccgagtat gaacagaatc tgatgcacta cgcgctatca cagctggaat ctgtaccgga
    19201 tctcactctc tatggcccgc ggaacaggct tggcgttatt gcttttaatc ttggtaaaca
    19261 ccacgcctat gatgttggca gttttctcga taactacggc attgctgtgc gtaccggaca
    19321 tcactgcgct atgccattaa tggcctatta caacgtccct gctatgtgtc gggcgtcgct
    19381 ggccatgtat aacacccatg aagaagtgga tcgtctggtg accggactgc aacgtattca
    19441 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    19501 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    19561 ccagcgtctg ccagaattac gcgccgaaga ccgaagccca caaaatagca ttcagggctg
    19621 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    19681 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    19741 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    19801 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    19861 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    19921 tgccggatgg tcaatgttta cactgacctg cacaggagtg ttttggtttc aggtgaacat
    19981 aagcgccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    20041 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    20101 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    20161 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcggcaa
    20221 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    20281 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    20341 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    20401 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    20461 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    20521 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    20581 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    20641 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    20701 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    20761 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    20821 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    20881 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    20941 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    21001 aaccagagca agggaatatg ttacgcgcga cgcagtagcg gtaaacggca gacaaaaaaa
    21061 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    21121 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    21181 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    21241 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    21301 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    21361 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    21421 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    21481 gataaaaaaa ttgagagaaa acaggatgct tccatcggat tcatcttaga taaagtgaga
    21541 ttatatagtg aaat
//
LOCUS       DAZFYC010000077        21536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFYC010000077 DAZFYC010000000
VERSION     DAZFYC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.077"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..1675
                     /gene="caiT"
                     /locus_tag="ACHKW5_003279"
     CDS             161..1675
                     /gene="caiT"
                     /locus_tag="ACHKW5_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO7512619.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1706..2848
                     /gene="caiA"
                     /locus_tag="ACHKW5_003280"
     CDS             1706..2848
                     /gene="caiA"
                     /locus_tag="ACHKW5_003280"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO7512620.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2977..4194
                     /gene="caiB"
                     /locus_tag="ACHKW5_003281"
     CDS             2977..4194
                     /gene="caiB"
                     /locus_tag="ACHKW5_003281"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO7512621.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4268..5821
                     /gene="caiC"
                     /locus_tag="ACHKW5_003282"
     CDS             4268..5821
                     /gene="caiC"
                     /locus_tag="ACHKW5_003282"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO7512622.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5930..6715
                     /gene="caiD"
                     /locus_tag="ACHKW5_003283"
     CDS             5930..6715
                     /gene="caiD"
                     /locus_tag="ACHKW5_003283"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO7512623.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            6721..7311
                     /gene="caiE"
                     /locus_tag="ACHKW5_003284"
     CDS             6721..7311
                     /gene="caiE"
                     /locus_tag="ACHKW5_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO7512624.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7397..7792)
                     /gene="caiF"
                     /locus_tag="ACHKW5_003285"
     CDS             complement(7397..7792)
                     /gene="caiF"
                     /locus_tag="ACHKW5_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO7512625.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8053..11274)
                     /gene="carB"
                     /locus_tag="ACHKW5_003286"
     CDS             complement(8053..11274)
                     /gene="carB"
                     /locus_tag="ACHKW5_003286"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO7512626.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11292..12440)
                     /gene="carA"
                     /locus_tag="ACHKW5_003287"
     CDS             complement(11292..12440)
                     /gene="carA"
                     /locus_tag="ACHKW5_003287"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO7512627.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12896..13717)
                     /gene="dapB"
                     /locus_tag="ACHKW5_003288"
     CDS             complement(12896..13717)
                     /gene="dapB"
                     /locus_tag="ACHKW5_003288"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO7512628.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(13884..14798)
                     /gene="rihC"
                     /locus_tag="ACHKW5_003289"
     CDS             complement(13884..14798)
                     /gene="rihC"
                     /locus_tag="ACHKW5_003289"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO7512629.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(14864..15814)
                     /gene="ispH"
                     /locus_tag="ACHKW5_003290"
                     /gene_synonym="lytB"
     CDS             complement(14864..15814)
                     /gene="ispH"
                     /locus_tag="ACHKW5_003290"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO7512630.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15816..16265)
                     /gene="fkpB"
                     /locus_tag="ACHKW5_003291"
     CDS             complement(15816..16265)
                     /gene="fkpB"
                     /locus_tag="ACHKW5_003291"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7512631.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMEAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16353..16847)
                     /gene="lspA"
                     /locus_tag="ACHKW5_003292"
     CDS             complement(16353..16847)
                     /gene="lspA"
                     /locus_tag="ACHKW5_003292"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO7512632.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16847..19663)
                     /gene="ileS"
                     /locus_tag="ACHKW5_003293"
     CDS             complement(16847..19663)
                     /gene="ileS"
                     /locus_tag="ACHKW5_003293"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO7512633.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19706..20647)
                     /gene="ribF"
                     /locus_tag="ACHKW5_003294"
     CDS             complement(19706..20647)
                     /gene="ribF"
                     /locus_tag="ACHKW5_003294"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO7512634.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20655..20873)
                     /gene="yaaY"
                     /locus_tag="ACHKW5_003295"
     CDS             complement(20655..20873)
                     /gene="yaaY"
                     /locus_tag="ACHKW5_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO7512635.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20976..21239
                     /gene="rpsT"
                     /locus_tag="ACHKW5_003296"
     CDS             20976..21239
                     /gene="rpsT"
                     /locus_tag="ACHKW5_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO7512636.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5126 a         5789 c         5541 g         5080 t
ORIGIN      
        1 aaaaatgtga taacaatcac agaatgcagc ttattgaata cccattatga gttagccatt
       61 aacgcgtcca cgaggttaat aataattata ttaaatgtta acaaaaataa aacaaacggg
      121 aacgcaaaat aaatattcgt tttcacagtg gaattaactc atgaagaatg aaaagagaaa
      181 aacgggaata gaaccgaagg ttttctttcc gccgttaata atcgtcggca tactttgttg
      241 gcttacagtc agagatctgg atgcagcgaa tgtcgttatt aatgctgtat tcagttacgt
      301 caccaatgta tggggatggg catttgaatg gtatatggtg gtgatgcttt tcggttggtt
      361 ctggctggtg tttggcccgt atgccaaaaa gcgtttaggt aacgaaccac ctgaatttag
      421 taccgccagt tggatcttta tgatgttcgc ctcctgtacg tctgctgccg tactgttctg
      481 gggatcgatt gagatctact actacatctc caccccgccg tttggcttag aaccgaactc
      541 gacaggggcg aaagagttgg ggctggctta cagcttgttc cactggggac ctctgccgtg
      601 ggccacttac agcttccttt cagtcgcctt cgcttacttc ttctttgtcc gcaaaatgga
      661 agtgattcgc cccagctcga cactggtgcc gctggtaggt gaaaaacacg ccaaagggtt
      721 gttcggcact atcgtcgaca acttctatct cgtcgccttg atcttcgcga tgggtaccag
      781 tctgggcctt gccacgccgc tggtgaccga gtgtatgcaa tggttgtttg gcattccgca
      841 taccctgcaa ctggacgcta tcatcattac ctgctggatt atcctcaacg ccatttgcgt
      901 cgcttgcggt ctgcaaaaag gggtacgtat cgccagtgac gtgcgtagtt acctgagctt
      961 cctgatgctg ggttgggtgt tcattgtcag cggtgccagc ttcatcatga actacttcac
     1021 cgattcggtg gggatgttgc tgatgtatct gccgcgcatg ttgttctata ccgatcccat
     1081 cgctaaaggc ggcttcccgc agggctggac cgtgttctac tgggcatggt gggtgattta
     1141 tgctatccag atgagtatct tcctcgcccg catctcccgt ggtcgtactg tgcgtgaact
     1201 gtgcttcggc atggtgctgg ggctgacagc gtcaacctgg atcctgtgga ctgtactcgg
     1261 tagtaacact ctgctgttga tagataaaaa cattatcaac attccaaatc tgatcgaaca
     1321 gtacggtgtg gcgcgcgcca ttatcgaaac ctgggccgct ctgccactca gcaccgccac
     1381 catgtggggc ttcttcatcc tctgctttat tgccaccgtt acgctggtta acgcctgctc
     1441 ttataccctg gcgatgtcca cttgccgcga agtacgcgat ggtgaagaac caccgctgct
     1501 ggtgcgtatc ggttggtcaa ttctggttgg cattatcggt attgttttgc tggcgctcgg
     1561 cggcctgaaa ccgattcaaa ctgccattat cgccggagga tgcccgctgt tcttcgtcaa
     1621 cattatggtg acgctctcct ttattaaaga cgcgaaacag aactggaaag attaattaac
     1681 ccccaaaata tcaagaggtt gaaagatgga ttttaattta aatgatgagc aggaactgtt
     1741 tgtcgccggt atccgcgaac tgatggccag cgaaaactgg gaggcctatt ttgccgagtg
     1801 cgaccgtgac agcgtctacc cggaacgttt tgtcaaagca ctggcggata tgggtatcga
     1861 cagtctgctg atccctgaag agcacggtgg tctggacgcg gggtttgtta ctctcgccgc
     1921 cgtgtggatg gagctgggac gtctgggggc accaacctat gtactgtacc agttgccggg
     1981 cgggttcaac accttcctgc gcgaaggcac acaagagcag atcgacaaga ttatggcttt
     2041 ccgcggcacc ggtaagcaga tgtggaactc agcgattacc gaaccgggcg cgggctccga
     2101 cgtgggtagc ctgaaaacga cttatacccg tagaaatggt aagatttatc ttaatggtag
     2161 taagtgtttt attaccagca gcgcctacac cccgtacatc gtggtgatgg cgcgcgacgg
     2221 ggcttctccg gacaaacctg tctacaccga atggtttgtt gatatgagca aaccgggcat
     2281 caaagtgacc aaacttgaaa agctcggtct gcgtatggat agctgctgtg aaatcacctt
     2341 tgacgacgtg gaactggacg agaaagacat gttcggtcgg gaaggtaacg gctttaaccg
     2401 cgtcaaagaa gagttcgacc atgaacgttt cctggtagcc ctgaccaact acggtacggc
     2461 gatgtgcgcc tttgaagatg cggcgcgcta cgccaaccag cgcgtgcagt ttggcgaggc
     2521 tattggtcgt ttccagttga ttcaggaaaa attcgcccac atggcgatca aattaaactc
     2581 catgaaaaac atgctgtatg aagcagcgtg gaaagcagac aacggcacca tcacctctgg
     2641 cgatgcagcg atgtgcaaat acttctgcgc caatgcggca tttgaagtgg tggatagcgc
     2701 aatgcaggtg ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc gcttctggcg
     2761 tgacctgcgt gtagaccgcg tctccggggg atctgacgaa atgcagatcc tgacgctggg
     2821 tcgtgcggtg ctgaagcaat accgctaagt caatgcgctg cctgatgcga cgctgatgcg
     2881 tcttatcagg cctacgggag cacgaatgta ggccggataa agcgtttacg ccgcatccgg
     2941 cagtcatgcg ctacataaat ttttcaggag caaataatgg atcatctacc catgccgaaa
     3001 ttcgggccgt tggccggatt gcgcgttgtc ttctccggta tcgaaatcgc cggaccgttt
     3061 gccgggcaaa tgttcgcaga atggggcgcg gaagttatct ggatcgagaa cgtcgcctgg
     3121 gccgacacca ttcgcgttca accgaactac ccgcaactct cccgccgcaa tttgcacgcg
     3181 ctgtcgttaa atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt aatggaaacc
     3241 accgatatct tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat
     3301 gaagtactgt ggcagcacaa cccgaaactg gttatcgctc acctgtccgg ttttggtcag
     3361 tacggcaccg aggagtacac caatcttccg gcctataaca ctatcgccca ggcctttagt
     3421 ggttacctga ttcagaacgg tgatgttgac cagccaatgc ctgccttccc gtataccgcc
     3481 gattactttt ctggcctgac cgccaccacg gcggcgctgg cagcactgca taaagtgcgt
     3541 gaaaccggta aaggcgaaag tatcgacatc gccatgtatg aagtgatgct gcgtatgggc
     3601 cagtacttca tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat gagcaaaggt
     3661 aaagatccct actacgccgg ttgcggtctg tataaatgcg ccgacggcta catcgtgatg
     3721 gaactggtgg gcattaccca aattgaagag tgctttaaag atattggcct cgcacatcta
     3781 ctcggtacgc cggaaatccc ggaaggcact cagcttatcc accgtatcga atgcccttac
     3841 ggcccactgg tagaagaaaa actcgatgcc tggctggcgg cacataccat cgcagaagtt
     3901 aaagaacgct ttgccgaact gaatatcgcc tgtgccaaag tactgaccgt accggaactg
     3961 gaaagcaatc cacagtatgt tgcccgtgaa tcaatcactc agtggcaaac gatggatggt
     4021 cgcacctgca aagggccgaa catcatgccg aaattcaaaa ataaccccgg acaaatctgg
     4081 cgcggaatgc cctcacatgg catggacacg gctgccattt tgaaaaatat cggctacagc
     4141 gaaaacgaca ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt
     4201 ggcgtcagtc ggcttgcctg actagcgccc gcccaataac aaacgaacaa aaatggatag
     4261 aggtgcaatg gatatcattg gcggacaaca tctacgtcaa atgtgggacg atcttgcgga
     4321 cgtttacggt cataaaacgg cgctgatttg tgaatccagc ggcggagtcg ttaaccggta
     4381 tagttatctt gagttaaatc aggagattaa ccgcacggca aacctgtttt atacgctggg
     4441 gattcgcaaa ggcgacaagg ttgcactaca tctcgacaac tgcccggaat ttatcttttg
     4501 ctggttcggg ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc gcctgttacg
     4561 cgaggaaagc gcgtggatcc tgcaaaatag ccaggcgtgc ctgctggtga ccagtgcgca
     4621 attctatcct atgtatcaac agattcagca ggaagatgcc actcaattgc ggcacatttg
     4681 cctgacagat gtggcacttc ccgctgatga tggcgtgagt tcgtttactc aactgaaaaa
     4741 tcaacaacct gccaccttgt gctatgcacc gccgctatcg actgacgata cggcggaaat
     4801 tctcttcacc tccggcacca cctcccgacc gaaaggtgtg gtgattaccc attacaacct
     4861 gcgcttcgct ggatattact ccgcctggca gtgtgcactg cgtgacgatg acgtctacct
     4921 gacggtaatg cctgcgtttc atatcgattg ccagtgtact gcggcgatgg cggcgttttc
     4981 tgccggggcc acctttgtgc tggtcgagaa atacagcgcc cgcgccttct ggggacaggt
     5041 gcagaagtac cgcgccacca ttaccgaatg tattccgatg atgatccgta cgttgatggt
     5101 gcagccgcct tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt tttatctcaa
     5161 cttgtcggag caggaaaaag atgcgttttg tgaacgcttc ggcgttcgct tgctgacgtc
     5221 ttatgggatg acggaaacca ttgtgggcat tatcggcgat cgccctggcg ataaacgacg
     5281 ctggccgtcg attggtcggg cggggttttg ctacgaagcg gagatccgcg acgatcacaa
     5341 tcgcccgctc ccggcaggtg agatcggtga aatctgtatt aaaggcgtac cagggaaaac
     5401 catcttcaaa gagtattttc tcaacccgaa agccactgcg aaagtgctgg aagccgacgg
     5461 ctggctacat accggcgata ccggatactg cgacgaagag ggcttttttt atttcgtcga
     5521 tcgccgctgc aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg agctggaaaa
     5581 tatcatcgcc acccatccaa aaattcagga tatcgtggtt gtgggtatta aagattcgat
     5641 tcgcgatgaa gccatcaaag catttgtggt gctgaatgaa ggtgaaacat tgagcgaaga
     5701 ggaatttttc cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct cttatctgga
     5761 gatcagaaaa gatctgccac gtaattgctc ggggaaaata attagaaaga atctgaaata
     5821 aatgaaacag cagggaacta ccctgccagc taataatcac gctataaaac aatatgcatt
     5881 ttttgcaggg atgttgtcgt ccctgaaaaa gcaaaaatgg agaaaaggaa tgagtgaatc
     5941 attacatctg acccgcaatg gatcaattct ggaaattacc cttgatcgtc caaaagcgaa
     6001 tgctattgat gcaaaaacca gctttgaaat gggcgaagta tttctaaatt tccgtgacga
     6061 tccgcaatta cgtgtcgcca ttattaccgg tgccggagag aagttctttt ccgcgggctg
     6121 ggatttaaaa gcggcagcag aaggcgaagc accggatgct gactttggcc cgggtggatt
     6181 tgcgggatta accgaaattt tcaatctcga caaaccggtt atcgcagctg tgaacggcta
     6241 tgcctttggc ggcggctttg aactggcgct ggcggcagat tttattgttt gtgccgataa
     6301 tgccagcttc gccctgccgg aagccaaact gggcatcgtt cctgacagcg gcggcgtgct
     6361 gcgtctgccg aagatcctgc cgcctgccat cgtcaatgaa atggtgatga ccggcagacg
     6421 aatgggcaca gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta gccaggcgga
     6481 actgatggat aacgcccgcg aactggcgca gcagctggtt aacagcgccc cgctggcgat
     6541 tgcggcgctg aaagagatct accgcaccac cagcgaaatg ccggtagaag aagcgtatcg
     6601 ctatattcgc agcggcgtgt tgaaacacta tccgtcggtt ctgcattcgg aagatgccgt
     6661 tgaagggccg ctggcgtttg ccgagaagcg cgatccggta tggaaaggac gttaacaacc
     6721 gtgagctatt acgcctttga ggggttaatt ccggtggttc acccgacggc gtttgtccat
     6781 cccagtgccg tcttgattgg cgatgtgatt gtgggagccg gtgtctacat cggcccactc
     6841 gcctcactgc gtggtgacta cgggcggttg atcgtgcaag cgggagccaa tattcaggat
     6901 ggctgcatta tgcatggcta ctgcgacact gacaccatcg ttggggaaaa cggccatatc
     6961 gggcacggag cgatcctgca tggttgtgtg attggtcgcg atgcattggt cgggatgaac
     7021 agcgtgatta tggatggcgc ggtcattggc gaagagagca ttgttgccgc catgagcttt
     7081 gtcaaagcag gctttcacgg cgagaaacgc cagttgttga tgggtacgcc agcccgcgct
     7141 gtacgcagtg ttagtgacga cgagttacac tggaaacggt tgaataccaa agagtatcag
     7201 gatcttgtcg ggcgctgtca tgcatcgtta catgaaacac agccactgag gcaaatggag
     7261 gaaaatcgcc cccgtttgca ggggacgacg gatgtgacgc ctaaacggtg atcttgccgg
     7321 atgcgacctg gttgctggat aagatgctac aacatcgcat ctggcaagca ggacgcccgg
     7381 tatcaacgag ataaaattaa cgacgcatac tctttgacaa catcatctgc cacttctgct
     7441 cgcggttaag ttccgtgagc ggaacggcag gaatacgcgg cgtctttctt acgccgacta
     7501 atttatcccg gctgttattg cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt
     7561 ttaccagtcg ctggcactgg catccccgtc cttccagctt attagggatc atcttaactt
     7621 cgcagcttat ttctgcgact tccgacagaa tataagtcag ggtattaacc gccttgctgt
     7681 gttcaatatc gaaatgaata gagatctctc ttgctatcac ccaccgattt tcagccatca
     7741 tccattcggc gattaacaag tagagtggtt tttcaacata tccttcacac atacgaactc
     7801 cagtttcaca aattaaaagt ctggctatga ctcgttttga agcaattaaa caaggccata
     7861 ttaaaattgt ggatatatcg atagtatgca acttcaccgt cgataagtgt gagctaaatc
     7921 ctgcttatgt catcaaaaat atcgattccc caaaagattt acttaatggg caaaaaaaac
     7981 ggtcctcatc agagaaccgt attacttatt cgatcaaatt agcggatgaa aaatatccgc
     8041 catgacacgc tattatttga tctgcgcgtg catttcctgc accgaaatta ctttttcggt
     8101 cgcgtcggca ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg
     8161 cactttatat tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cgcgacggcc
     8221 tgaagtggtg ttgatgatgt aggtatattc gccattcttg atgcggtcct gaatgtgcgg
     8281 acggccttca tgcaccttgt tcaccagacg cggattaatg cccgcttcgc ccagcacaat
     8341 cgccgtgccg tgggtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc
     8401 caccacgcgt tctttatcgc cttcgcgcac ggaaagcaac gcacgaccgt gtttcttcat
     8461 ggtggagttg ctgcccagct gcgctttggc aaacgcttca gcgaaggtgc ggcccacgcc
     8521 catcacttcc ccggtggagc gcatttctgg ccctaacagc gggtcaacac ccgggaattt
     8581 attgaacggc agcaccactt ctttcaccga gtagtacggc gggataactt ctttggttac
     8641 gccctgctca gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac
     8701 gccggtggct ttggagacga acggaacggt acgcgccgca cgcgggttaa cttcaatcag
     8761 gtagacttcg ttgtttttca ccgcaaactg cacgttcatc agaccgcgca cctgcaattc
     8821 gaaggccagt ttctgcacct gctggcgcat cacatcctga atttcctgac ttaaggtgta
     8881 ggctggcaga gaacatgcgg agtcaccgga gtgcacgccc gcctgctcga tgtgctccat
     8941 gatgccgcca atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctaccgc
     9001 gtcatcaagg aagtgatcca gcaacactgg cgcatcgtta gacacgctga ccgccgtctg
     9061 gaagtagcga cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac
     9121 gtaagacgga cgtaccacca gcgggtagcc aatctctttc gccttctcta ccgccatttc
     9181 aatagcggta acggtggcgt tcgccggttg tttcagtttc agacgctcaa ccgcatgctg
     9241 gaagcgttca cggtcttctg cacggtcgat agcatccggg ctggtgccga taaccggtac
     9301 gccagcagct tccagcgcgc gcgccagttt cagcggggtc tgaccgccgt actgaacgat
     9361 aacgcctttc ggcttctcaa tacgcacgat ttctagcaca tcttccagag ttaccggctc
     9421 gaagtagagg cgatcggaag tgtcgtagtc ggtggagacg gtttccgggt tacagttaac
     9481 cataatggtt tcgtaaccgt cttcgcgcag cgccagcgag gcgtgtacac agcagtagtc
     9541 gaattcgata ccctgaccga tacggttcgg gccgccgccg agaaccatga ttttttcacg
     9601 gtcggtagac ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc
     9661 ggtggcgaac tctgccgcac aggtatccac gcgcttataa accgggtgca ggtcatactg
     9721 gtcgcgcagc ttgcggattt ccgcttcgcg cacgcccgcc agttttgcca gacgcgcatc
     9781 ggcaaagcct ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac
     9841 ttccgctact ttctcttcca gacgcaccag ttcttcaatc tgtaccagga accagcggtc
     9901 aatattggtc aggttgaaca cgccgtccac agacaggccc gcacggaagg catcggcgat
     9961 gtaccagata cgctctgcgc cagcatcttt cagttcgcga cggattttgg ttaacgcttc
    10021 cgggtcatcg aggctcactt tcgggtcgaa tccagtcgca ccgacttcca ggccgcgcag
    10081 cgctttttgc agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt
    10141 catctgagtg gtcagacggt cgttagcacc agcgaatttt tcgaagttga agcgaggaat
    10201 tttagtaacc acatagtcga tggacggctc gaaggaggcc ggagtacgtc cgccagtgat
    10261 gtcgttcatc agttcgtcga gggtgtaacc caccgccagt ttcgccgcca ctttagcaat
    10321 cgggaaaccg gtcgctttcg acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc
    10381 gataacaatc aggcgaccgt ttttcgggtt caccgcaaac tggacattgg aaccaccggt
    10441 ttcaacgccg atttcacgca gcaccgccat cgaggcgtta cgcatgattt gatattcttt
    10501 gtcggtcagc gtttgggctg gcgcgacagt gatggagtca ccggtatgga tgcccatcgc
    10561 atcaaagttt tcgatagagc agacgatgat gcagttgtcg tttttatcac gcaccacttc
    10621 catctcgtac tctttccagc cgatcagcga ctcatcaatc agcagctctt tggttgggga
    10681 gagatccaga ccgcgggcgc aaatttcttc aaactcttcg cggttataag cgataccgcc
    10741 gccgctaccg cccatggtaa aggatgggcg aataatgcac gggaagccca cgtcagcggc
    10801 aaccgccagc gcttcttcca tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat
    10861 tttcttcatc gctacgtcga aacgacggcg gtcttctgct ttatcaatcg catcggcggt
    10921 cgcaccaatc atagtcacgc cgaactcttc caacacgccc tgacgctcca gctccagcgc
    10981 gcagttcagc gccgtctgac cgcccatcgt tggcagcacc gcgtccgggc gctctttttc
    11041 aataatcttg cgtactactt cccagtgaat cggctcgatg taggtcgcat cggccatttc
    11101 cgggtcggtc atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc
    11161 gcgcagggct ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac
    11221 aatcgggccc gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct
    11281 tttactcctg attacttagc ggttttacgg tactgctcaa ttaactcgat aaagtggtcg
    11341 aacaacggcg ctgcgtcgtg tggcccaggg ctggcttccg ggtgaccctg gaagctgaac
    11401 gccggtttat cagtgcgatg aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg
    11461 acacgcaggt ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg
    11521 atcatcacca cgtttttctc cacatcttta accggatggt tgccgccgtg gtgaccaaat
    11581 ttcattttga cagtcttcgc accgctcgcc agcgccagca gctgatgacc gaggcagatg
    11641 ccgaataccg gaatatcggt ttcgaggaat ttctggatgg cggtaatggc gtaatcgcac
    11701 ggggccgggt cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca
    11761 tcttccgcag aagtttgcgc cggaacgatg gtcaggcgac agcctctatc caccagcatc
    11821 cgcaggatgt tgcgcttggc accaaaatca taagccacga cgtggaacgg cagctcgtct
    11881 tcttttttcg cttctggcag gccgccggtc aacgtccagc tcccttgtgt ccagctatag
    11941 gcttctgcgg tggtcacttc ttttgccaga tccatgccat tcagacctgg gaacgcgcgg
    12001 gctttttcta acgccaacgc cgcatccggg ttatcgcctg cgataatgca gccattctgt
    12061 gcgcctttct cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg
    12121 atgttatggc gcttcaggta agaagagagg tcttcggtat tacggaagtt gctggcaatc
    12181 agcggcagat cgcgaatcac cagaccttgt gcatgtacct gagaagattc ttcatcggcg
    12241 tcattagtgc cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag
    12301 gaaggatcag tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca
    12361 accgccgaac ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc
    12421 aatagcgctg acttaatcaa aacaccctcc agagaatatt cactcacttt atttgcatat
    12481 taattcacaa tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac
    12541 ggcggcattc tggagatatg ggcgctaaaa gtcaacttaa tggcgggatt ttttatatta
    12601 ttttgtggct catgcatgtt ttacaggcat taagcgacaa aaagatcata taacctgttc
    12661 tggaaaacgc ttgcgcagca agaagattgc atgaaataaa agcgagcagg gaaaagtgga
    12721 ccaaatggtc aaaatttaca ttaaaacaac aaaaaccgaa gacaaaacat gtaatttttg
    12781 gcacttatag acaatttaaa aacataacat taaaaataaa agggccaaaa attaaagccc
    12841 tttataatca ataaattaaa tgtgttattt ttgcaccata acaaatattt tgtggttaca
    12901 aactattgag atcaagcaca tctcgcatat caaaaagacc actttcctta ccactcaacc
    12961 acaaagccga tcttaccgcg ccgttagcaa atgtcatgcg gctggacgcc ttatgagtga
    13021 tctccagacg ctcgccaata tcggcaaaca tcgcggtatg ttcaccaacg atgtcacctg
    13081 cacgcacggt ggcaaaacca atggtgccag gcacacgttc accggtgtgg ccttcacgac
    13141 tgtagaccgc gcaatctttc agatctttat caagggcgtg ggcgatcgcc tctcccattg
    13201 ccagtgcggt gcctgacggc gcatcaactt tatgtctatg atgtgcttca ataatttcga
    13261 tatcggtgta gtcacccatc actttggctg ctttctccag cagcttaagc atgacgttaa
    13321 cgccaacgct aaaattggcg gcaaagacaa tcgcaatatc ggcggcggcg tcacgaatcg
    13381 cctgtttacc ggcttcgtca aaccccgtag tgccgatcac catccctttg ccatgctggc
    13441 gacaaaaagc gagatggttc agcgtacctt ccggacgggt aaaatcgata aacacatcaa
    13501 aatcatcttt taccgcatcg aggctgcttt gcacggtaac gcctgttttc cctgcaccag
    13561 ccagctcacc ggcgtcgctg cccagtaaag aagatccttc gcgctccagc gccgcgccca
    13621 gctgcacgcc ttctaatgcc agcgccgcct gaatcaactg gcggcccata cgccccccgg
    13681 ctcccgcgat ggcaacgcgg atgtttgcat catgcatagc tattctcttt tgttaatttg
    13741 catagaccgt tttcagagta accagcgcat cgctacgccg ccagccaaaa acgctgataa
    13801 ttagaaatta atgataaaca gggagggata tcagtgaaag gcatgagtcg atgaatgact
    13861 gcatgccaat aacatgtgac aggttacgac gccagagcca gcacctcagc cacccactgc
    13921 tggaagcctt tcacatccag atccaatgcc acctgtacat tggctggctt gcccaggcaa
    13981 ccgtcgatat caaccaccgt cgtgcctgag gtaaattcgc cctgagtttc cactgccaca
    14041 aaacagggtt tgagagtgaa caggtccggg cgcaccagcc aggcgatggc gcagagatcg
    14101 tgcattcgca gaccgctttg catactgccg ctacggtagt ggctaaacag tgcatgaagc
    14161 atttttccgg tgcgattcaa ctccggcaaa gtagcgagat agtcaggggt taatattgcc
    14221 tgattggtga catccagacc gcacatgacg atttcgattc cgctacggaa gacacaggca
    14281 gccgcttctg gatcggcagc gatattaaac tcggcgtttg gcgtacagtt gccgcgtccg
    14341 gcagaaccac ccatgatcac cagacggcga atatacggct tgcattccgg gcattgtgaa
    14401 agtaacagcg caatattggt taacgggccg atggccacca gggtaacagg ctctggtgca
    14461 cgcatcaggg catcccgaat cgccagaaac gccggtatcc cgagcggctt tcggttgtgc
    14521 tcaacaaagt cgtagccagc cattcccgat tcgccgtgca cagatgccgc atcacgcggt
    14581 gcgcgtacca gcggcacagc ggccccttgg gcgagcggaa tctccgcatt ccagaaatgc
    14641 agcagttgca aggcattacg ggtagttttc tcaaccgaga cattacccgc gacggtggtc
    14701 atcagttgca ggtcgagttc gggggcaaaa atcgcggcgg caatggcgac ggcatcgtca
    14761 atgccggggt cagtatcgag gaagataggt aaacgcatgt tttctccata aaaaatgccg
    14821 gtaacaagac cggcattttc gcataactta ggctgctaat gacttaatcg acttcacgaa
    14881 tatcgacacg cagctctttc ggcacttcga aaacaatgtt ttcttcacgg ccttccagcg
    14941 gaatggcttc accaccgccc agctgctgca aacgtgccac cacattctgc accagaatat
    15001 ccggagccga tgcgcccgca gtcacgccga cgcatttaac ctctttcacc cactcttcct
    15061 ggatgtcttt cgcatcgtca atcaaaaacg cgcgtttgcc catacgctgg gccagctccg
    15121 ccagacggtt ggagttggag gagtttttcg aaccgaccac caacacaact tccgcctgtt
    15181 ctgccagggc gcgtaccgct tcctgacggt tagtcgtggc gtagcagatg tcatctttgc
    15241 gcggaccgac aattttcggg aagcgtttac gcagcgcgtc gatcacatca gacgtgtcat
    15301 ccaccgacag cgtggtctgg gtcataaagg agagcttctc ttcgtttttg accgtcagtt
    15361 tccacacatc gtccggcgat tcgaccagat acattccccc ttccgggtta ctgtactggc
    15421 ccattgtccc ttccacttcc gggtgcccgg cgtgaccgat gagaatagat tcttcgccac
    15481 ggcgactggc gcgggcgact tccatatgca ctttggtcac cagcggacag gtcgcgtcaa
    15541 ataccgtcaa atcgcgactt tttgcttcgt tacgtaccgc ctgagaaaca ccgtgtgcgg
    15601 agaaaatcag gatcgcgccg tccggtactt cgctaatctg ctcaataaag atagccccac
    15661 gctcacgcaa gctatcgacc acatagcggt tatgtaccac ttcgtgacgg acatatatcg
    15721 gtgcgccgta aatggccagc gcgttttcaa caatgctgat agcgcggtct accccggcac
    15781 aaaaaccacg cgggttggcc aacaggatct gcatgttacg cctccagtgc cggatcgatt
    15841 tccagcactt caatatcaaa atgaacggtc tgcccggcca gcggatggtt gaaatcaacg
    15901 gtaatggagt cgccgttaat ttcgcggatc acgccaggca tctcactgcc atccattgcg
    15961 gtaaaaagca tgattgcgcc aatttctggc tcgcctgcct ccataaattc acggcgggag
    16021 aagtactgaa tcaggtccgg tgacggcacg ccaaacgccg catctggctc caacgagaag
    16081 gtggttttat cgcccacttt cagccccaac aggtgttgct ccagcccttc agaaagagaa
    16141 gcatcaccca ggcggaacag cgccggttta ccgttgttgc gggtagactc ggcggtggtg
    16201 ccatcgtcga gttttagcgt gaagtgcacc aggacggcgc tattgctctg tacagattca
    16261 gacatgcagg tttgctctta tattttgagg atttttgccg gatgcggcct acgatttcgc
    16321 agcaatctgt aggccttatc cggcagggtt tattattgtt ttttcgcttt agaaggcaaa
    16381 aaaccttcca gcacaatcag tgccgcaccg acacaaatgg cagtatcggc aaggttgaag
    16441 gtggcgaagt gccagtcgcc gacgtagaag tcgatcatat cgacaacgaa gccgtgccac
    16501 aggcggtcga acaggttgcc cagcgcgccg ccaataatca gcgcataagc gatattgttt
    16561 agcttctgcg tggccttcga gcgatacatc atcactgcca ggatcacgct aataccaatc
    16621 gcaataccgg caaagaacca acgctgccag ccgccgctat cggcaaggaa actaaacgcc
    16681 gcgccatagt tacgcgcata atgcagatta agcgacggga acagcgggac cgtatccccc
    16741 agagcaaagt tctggaggat caggtatttg ctgcccagat cgataatcag cacgactacc
    16801 accagccaca gccagcgtag ccctgttgaa cagatcgatt gactcatcag gcaaacttac
    16861 gtttttcacc gtcaccggcg acgttgctga cacagcggcc gcagatttct gcgtgttccg
    16921 ccaccttgcc gacatcctgg gtgtagtgcc agcagcgtgg gcacttctca ccttcggctt
    16981 tactcaacgc gactttcagc cctttgagta cttcgctctg ctgagcatca gcaggtgcgt
    17041 cgttatagtc tgcaacggta gcgccggagg tcaacaggac aaatcgtaat tcatcgccca
    17101 gcgcggtcag tttcgccgac agttccggtt ctgcatacaa ggttactgcc gcttccagcg
    17161 agccacccac tttcttgtcg gcacgcgctt gctcaatgac tttgttcact tcgccacgca
    17221 ctttcaacag ctcgtcccag aacgcatcgt tcatcgcttc actgtctgcc aggccaaaca
    17281 ggccttcgta ccactcaccg gtgaagacgt atttttcacg ttcgcccggc aggtagcccc
    17341 acacttcatc agcggtgaag gagaggattg gtgccatcca gcgcaccagc gcttctgcga
    17401 tgtgatacag cgcagtctgg cagctacgac gcgccacact gtccgctttg gcggtgtact
    17461 gacggtcttt gatgatgtcg aggtagaagg aacccatctc aacggagcag aagcgcatca
    17521 gacgctgtac cacttcgtgg aaatcgtatg cttcgtacgc cttgaggatg tcttcctgtg
    17581 ccgctttcgc acaacctacg gcccagcgat ccagtaccac catctcttcc ggtttcacca
    17641 tatcttttgc tggatcaaaa ccgttcaggt ttgccagcag gaagcgcgcg gtgttacgga
    17701 tacgacgata gctatcggca gcacgtttca ggatctcgtc agaaacggcc atttcaccgg
    17761 tgtagtcggt tgatgccacc cacagtcgca gaatatccgc gcccagtttg ttcatcacat
    17821 cctgcggcga aacggtattg ccgatggatt tagacatctt gcgaccctga ccatccacgg
    17881 taaagccgtg ggtcagtacc tgacgatacg gcgctttgcc tttcatcgcg gtggagatca
    17941 ttagggaaga catgaaccaa ccacggtgct ggtcagaacc ttccagatac atgtccgctg
    18001 cgtgaccggc aaattccgga cgcacgtcaa caacagaaga gtgggtagat ccggagtcaa
    18061 accatacatc caatgtgtcc ggcactttca cgtactgatc agcttcatcg ccgaggatct
    18121 ctttcgcatc aagatcccac cacgcctgga tgccgtcaac ttcaacgcgt tttgccactt
    18181 cttccattag ttcgagtgta cgcggatgca gctcttccgt gtctttgtgc acgaacagtg
    18241 acatcggtac gccccaggtg cgctgacggg agatacacca gtcaggacgg ttagcaacca
    18301 tcgactcgat acgcgcctgg ccccagtccg ggatccactg cacgcctttg atctctttca
    18361 gtgactgcgc acgcagacct ttctgatcca tgctgacgaa ccactgcggc gtcgcgcgga
    18421 agatgatcgg cgttttgtga cgccagcagc acggatagct gtgctgcatt ttctcaacgt
    18481 gcagcagcgc gcctttttcc tgcagcagcg caacgacgat gtcgttcgct ttgaagacgt
    18541 tcacgccatc cagcgtcgga taagtgcccg gcagataagt gccgtccggg ccaaccgggt
    18601 tagcggtttc caggccgtat ttctgaccga tcacatagtc gtccgggccg tggccaggcg
    18661 cggtgtgaac ggcaccggta ccggcatcca gggtaacgtg atcgccgagg attgccggaa
    18721 cgtcgaagcc cataaacgga tgggtaaagc gcagcaactc aagctccgca ccttttaccg
    18781 tgccgagaat ggtgtaatcg gtcacgccga tacgctgcat tacgctttca accagatctt
    18841 tcgccagaat cacggcctga ccgtcgatct gcaccagcgc atagtcgaaa tcaggtgcaa
    18901 tagagattgc gcggttcgca ggcagagtcc acggcgtagt ggtccagatc accagcgaga
    18961 ttgggccgtt aacgttgctt acggcaaatt ttgctttcag tgcatcctga tcgaccgcct
    19021 gaaaagcaac gtcgatggac ggagaagttt tgtcgtaata ctcaacttcc gcttccgcca
    19081 gcgcagaacg gcagtcaacg caccagtgaa ctggcttcgc gcctttgtgc aggtgaccgt
    19141 tgccgatgat tttgcccagc gcgcggatga tgttggcttc agttttgaag tccatggtca
    19201 ggtacgggtg cgaccagtcg cccagcacgc ccagacggat aaagtctttg cgttgaccgt
    19261 caacctgggt cgccgcgtat tcgcggcact tggcgcggaa ctcggcggcg gtgaatttct
    19321 cacccggctt accgtattct tgctctactt tcagctcgat cggcagaccg tggcagtccc
    19381 agccaggcac atacggcgag tcataaccgg aaagcccttt ggacttcacg ataatgtctt
    19441 tcagaatctt gttaaccgag tgaccaatat gaatgctgcc attcgcataa ggagggccat
    19501 catgcagaat gaaggttttt ttgccttttt tagccgcacg gatgatgccg tacagatcat
    19561 catcagtcca acgcgccagc attcccggtt cgcgcttggc gagatcgcca cgcatcggga
    19621 accctgtttc cggcaaattc agggttgatt tatagtcact catcagattc tcggttccgt
    19681 gtttcggttt gattacataa caggcttaag ccggttttgt tagcccaaaa aattcgcggg
    19741 cggttaattc atcacgcgca atctgcgctt tcagttcgtc cagcgacgca aatcgctgct
    19801 cattgcgtat ttttttacgc agcactactt gtatatggcg accgtaaagg tccattgcaa
    19861 catctaacaa atgcacttcc agctgctggc gaataccggc aaccgttggg cgtgttccaa
    19921 tgtttgccac gccgggtaac ggcttttcac cgaggcccag cacttctacc gcataaaccc
    19981 ctttcaccgg ggaaacctga cggcgtagcg gtacattcgc cgtcgggaaa cctatagtgc
    20041 gccctaattc atcaccgtgg actacacgcc cggagatggc aaacgggtgc cccagtaaac
    20101 tttctgccag agccagattg tcatccgcca gcgcctgacg cacagcggtg ctgctgatac
    20161 gcacgccacc ttcgcaaaaa gtttgcgtac tggtgatatc gaagccgtat tccatgccag
    20221 ctttctgtaa taacaagaaa tcgccttcac gaccagcgcc aaagcggaaa tcatcaccta
    20281 cggcaagaaa ttttacgcgc aaatgcttca ccagaagatc gctgatgaaa ttttgcgcgg
    20341 ttaacgccgc gaaacgcctg tcgaaacgca cgcacagcac gtaatcaacg ccacactctg
    20401 caaggtaacg cagtttttcc cgcagccggg tcagccgtgc cggggcttta tcggtagcaa
    20461 acagttccag tggttgaggt tcaaaaagca tcaccatcac cggtaagttg cgcttgcgcc
    20521 cttcttcctg caagccctgt aacagcgcgc gatgaccgcg atgcacgccg tcgaaattac
    20581 caatagtcag cacacaccct tcttgcgggg cctggctgag attatgtatg ccgcgtatca
    20641 gcttcatgtc tggctcaaaa cagtgaaaat cgtccgagta taccttgtac agcggtcaag
    20701 gttaaccggc gattgagtac cgaaatcaga aagaagtggc ttttcattgc catggcgcaa
    20761 atcacgggaa gaaagtgacc gcctgctgca atttttatcg cggaaaagct gtattcacac
    20821 cccgcaagct ggtagaatcc tgcgccatca ctacgtaacg agtgccggca cattaacggc
    20881 gcttatttgc acaaatccat tgacaaaaga aggctaaaag ggcatattcc tcggcctttg
    20941 aattgtccat atagaacaca tttgggagtt ggaccttggc taatatcaaa tcagctaaga
    21001 agcgcgccat tcagtctgaa aaggctcgta agcacaacgc aagccgtcgc tctatgatgc
    21061 gtactttcat caagaaagta tacgcagcta tcgaagctgg cgacaaagct gctgcacaga
    21121 aagcatttaa cgaaatgcaa ccgatcgtgg accgtcaggc tgctaaaggt ctgatccaca
    21181 aaaacaaagc tgcacgtcat aaggctaacc tgactgcaca gatcaacaaa ctggcttaat
    21241 cgccaatttg ctgaagcttt gtgaaaaagc ccgcgcaagc gggttttttt atgcctgctg
    21301 cttttatgat gcgtgagaga acacactacc ggttgccaga tagtcaaaag atgctggctt
    21361 ttgcagacat gccgttacac tttctgaact cttcgtggct taagaatcgt agaaccatcg
    21421 gctgatacat tctgaacaag aatatctact caatctccag actcccctac aaagaatgtg
    21481 gatattgtga tacacattga ggtattaaac ttaagaccac tctatttttg aattaa
//
LOCUS       DAZFYC010000078        21252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFYC010000078 DAZFYC010000000
VERSION     DAZFYC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.078"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            77..1132
                     /gene="phoE"
                     /locus_tag="ACHKW5_003297"
     CDS             77..1132
                     /gene="phoE"
                     /locus_tag="ACHKW5_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO7512637.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHKW5_003298"
     CDS             complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHKW5_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7512638.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHKW5_003299"
     CDS             complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHKW5_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7512639.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHKW5_003300"
     CDS             complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHKW5_003300"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7512640.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3685..5142
                     /gene="pepD"
                     /locus_tag="ACHKW5_003301"
     CDS             3685..5142
                     /gene="pepD"
                     /locus_tag="ACHKW5_003301"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7512641.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5199..5813)
                     /gene="prfH"
                     /locus_tag="ACHKW5_003302"
     CDS             complement(5199..5813)
                     /gene="prfH"
                     /locus_tag="ACHKW5_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO7512642.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5810..6949)
                     /locus_tag="ACHKW5_003303"
     CDS             complement(5810..6949)
                     /locus_tag="ACHKW5_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO7512643.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7195..7647)
                     /gene="yafP"
                     /locus_tag="ACHKW5_003304"
     CDS             complement(7195..7647)
                     /gene="yafP"
                     /locus_tag="ACHKW5_003304"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7512644.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7644..8699)
                     /gene="dinB"
                     /locus_tag="ACHKW5_003305"
     CDS             complement(7644..8699)
                     /gene="dinB"
                     /locus_tag="ACHKW5_003305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO7512645.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8770..9555)
                     /gene="lafU"
                     /locus_tag="ACHKW5_003306"
     CDS             complement(8770..9555)
                     /gene="lafU"
                     /locus_tag="ACHKW5_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO7512646.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPKSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9527..11239
                     /locus_tag="ACHKW5_003307"
                     /pseudo
     CDS             9527..11239
                     /locus_tag="ACHKW5_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11344..11622
                     /locus_tag="ACHKW5_003308"
     CDS             11344..11622
                     /locus_tag="ACHKW5_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO7512647.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            11615..11971
                     /locus_tag="ACHKW5_003309"
     CDS             11615..11971
                     /locus_tag="ACHKW5_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO7512648.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(12028..12801)
                     /gene="yafL"
                     /locus_tag="ACHKW5_003310"
     CDS             complement(12028..12801)
                     /gene="yafL"
                     /locus_tag="ACHKW5_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7512649.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12987..13247
                     /gene="dinJ"
                     /locus_tag="ACHKW5_003311"
     CDS             12987..13247
                     /gene="dinJ"
                     /locus_tag="ACHKW5_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO7512650.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            13250..13528
                     /gene="yafQ"
                     /locus_tag="ACHKW5_003312"
     CDS             13250..13528
                     /gene="yafQ"
                     /locus_tag="ACHKW5_003312"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO7512651.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            13684..14424
                     /gene="dpaA"
                     /locus_tag="ACHKW5_003313"
     CDS             13684..14424
                     /gene="dpaA"
                     /locus_tag="ACHKW5_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO7512652.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(14395..15162)
                     /gene="yafJ"
                     /locus_tag="ACHKW5_003314"
     CDS             complement(14395..15162)
                     /gene="yafJ"
                     /locus_tag="ACHKW5_003314"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO7512653.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMSGEWRLFCLGERVV"
     gene            complement(15368..15946)
                     /gene="lpcA"
                     /locus_tag="ACHKW5_003315"
     CDS             complement(15368..15946)
                     /gene="lpcA"
                     /locus_tag="ACHKW5_003315"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO7512654.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            16186..18630
                     /gene="fadE"
                     /locus_tag="ACHKW5_003316"
     CDS             16186..18630
                     /gene="fadE"
                     /locus_tag="ACHKW5_003316"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO7512655.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(18673..19119)
                     /gene="ivy"
                     /locus_tag="ACHKW5_003317"
     CDS             complement(18673..19119)
                     /gene="ivy"
                     /locus_tag="ACHKW5_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO7512656.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            19300..20067
                     /gene="yafV"
                     /locus_tag="ACHKW5_003318"
     CDS             19300..20067
                     /gene="yafV"
                     /locus_tag="ACHKW5_003318"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO7512657.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(20291..>21252)
                     /locus_tag="ACHKW5_003319"
     CDS             complement(20291..>21252)
                     /locus_tag="ACHKW5_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO7512658.1"
                     /translation="QYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDK
                     DVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNM
                     LDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPE
                     HDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCGAVSFRSIIAEQKKEP
                     EMTVRYYISSADLTAEKFATAIRNHWHLENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         5220 a         5406 c         5393 g         5233 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga ctggctatgg
      301 ccgttgggaa gcggagtttg ccggaaataa agcggagagt gatactgcac agcaaaaaac
      361 gcgtctcgct tttgccggat tgaagtataa agatttgggt tctttcgact atggccgtaa
      421 cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga
      481 ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg
      541 gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa
      601 aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata
      661 tgactttggc ggcagcgatt tcgccattag tggtgcctat accaactcag atcgcaccaa
      721 cgagcagaac ctgcaaagcc gtggcactgg caagcgtgca gaagcatggg caacaggtct
      781 gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac gcaaaatgac
      841 gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca
      901 gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga
      961 aggtatcggt gatgaagatc tggtcaatta tatcgatgtc ggggctacat attatttcaa
     1021 caaaaatatg tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt
     1081 gaatattaat aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatgattatt
     1141 gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg gctcgtcacg
     1201 aaaatcatcc gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagcttctc
     1261 gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac
     1321 gctcttccag tcgcctgctt tatcgaacag accaaactgg tagctgtagg taaaacggga
     1381 ttcctgcgct tccagctcca tccaccagcc ccagaattca cgcacttccg gtgccggttt
     1441 cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc
     1501 acgaatatac gggcctagtg cggtaaattt tttgatcaat ctgctcttcg ggtgtccact
     1561 cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaaattt
     1621 agcaaatttt taacacaagc gtttttcgat ccagccggtg atttcctgaa gacctttgtc
     1681 aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga
     1741 ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa
     1801 cattggcgtt gggcagcgac gtcccagcaa tccttgcact tttaatgaat agcgattcag
     1861 ctccacgcgc aacgcgtcat cggaagcatc atgcatcccc aaacgactcg ccagaacgtc
     1921 aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac
     1981 cggaccaaga caggcaaccg ctttcagacg cggcgattca aggtatgcca gacgcacggc
     2041 aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac
     2101 gttaggcagc gcctttaaga cgtgctgatg caacaggctg gagtcctggg tgagcttcca
     2161 ttttgaagaa aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc
     2221 aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat
     2281 taatactgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc
     2341 accgccgggt acggtaaact ccatctgccg catcgtgccc ggcagacgct gggcggcctc
     2401 ttcataggcg cggtttgata aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg
     2461 aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgtcgac cactgagttg
     2521 cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg cccactcata
     2581 aatccagttg ccaccacggt agccaatcac agtatcgtat aaatcatcgt cggtacgttc
     2641 agcatcgctc atcacaatac gtgcctggac gtcgaggatt tcacgtgggt caataccgcg
     2701 ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag
     2761 agccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc
     2821 aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc
     2881 agcctccaga atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc
     2941 ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc
     3001 ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca taatcatcaa
     3061 ccagcgggcg accggccggt tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt
     3121 cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt
     3181 cgccatcgcc ttctgcacgt ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc
     3241 tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg
     3301 gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc
     3361 ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc
     3421 tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag
     3481 gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt
     3541 ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc
     3601 aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc
     3661 gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg
     3721 tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc
     3781 gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta
     3841 ggtaatatcc tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc
     3901 ttacaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg
     3961 aaagatccta tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg
     4021 ggtgcggata acggcattgg tatggcctct gcactggcag ttctggctga cgaaaacgtg
     4081 gttcacggcc cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg
     4141 ttcggcttac agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa
     4201 gaaggtgaaa tctacatggg ttgtgcgggg ggtatcgact tcacctccaa cctgcattta
     4261 gatcgtgaag cggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa
     4321 gggggtcact ccggcgggga aatccacgtt ggcctgggta atgccaacaa actgctggtg
     4381 cgcttcctgg cgggtcatgc agaagaactg gatctgcgcc ttatcgattt caacggcggc
     4441 acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa
     4501 gtcgacgtcc tgaaatctct gatgaatacc tatcaggaga tcctgaaaaa cgagctggca
     4561 gagaaagaga aaaatctggc cttgttgctg gactctgtag cgaacgataa agctgccctg
     4621 attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc
     4681 cgcaactcag acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc
     4741 atgactgata ataacgtaga aattcactgt ctgatccgtt cactgatcga cagcggtaaa
     4801 gactacgtcg tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg
     4861 aaaggcgcat atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt
     4921 gaaacctatc agcgtctgtt caacaaaaca ccgaacatcc agattatcca cgcgggcctg
     4981 gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaact
     5041 atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg
     5101 acgctgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg
     5161 gatggcgttt aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc
     5221 cctgtaaatg tccgtctcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgc
     5281 gatttcagca ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat
     5341 cgtgccagcc gcttgttagc atgctgacta cgctctgact gaaccttcac gctaataccg
     5401 gatgccaaat gcgtggcgcg taccgccgag tcggttttat tgacatgttg accgcccggc
     5461 cccgacgaac gcaacgtctc ataacggatt gcatccgatt gttcctgctc atcagcggta
     5521 aaacgcccaa tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcgga
     5581 caaatccact gaatagcgcc acaccagctt tcgcttaatg cccatgcgtt atcgccatcc
     5641 agagaaatca gcgccgaacg cagcgtgtca gagtagcggc ccgtttctgt ttccaacacc
     5701 gttaccgcga cgtcttgtcg ggcagcttct ttaataagcc tgtccagcgc ctttttcact
     5761 gcgagacaac attcttccgg cccctgcgct gaggagagtt gtagcaagat catccttttt
     5821 tccctccact gtttttgagc gttagcaccg gacgcagccg cgcgacagga ataattaaac
     5881 cggcctgcac cagacattgc accacgcttt cagccgattt ataggcttgt ggcgcttctt
     5941 caaagatgag ttgtttatcg cgacaaatta cccgactgcc aagttcagta cgcgagagtt
     6001 gcgtcgccgt gtatttcgct gccagacgcc ctttacactc ggtgcgcccc catttacgcc
     6061 ctgccccatg cgccagcgag tggagcgttt tttcgttcgc aacaggctgt acaatccagg
     6121 agtaatcacc acgtgaaccg ggaataatca ccagaccgtt gtcatccggc gtggcacctt
     6181 tacgatgcaa ccagccctgt tgatcgccga tttgacacgc gctaacaaag ttatgcgcca
     6241 catccagaac cggacttccc gtggccttaa cttgttgcat tatgcgcaaa gcgatcatct
     6301 ggcgattaat acacgcaaac gccagcgcat cgtcatgttc cgcaatatac cgtagcgcgt
     6361 cgtcactgcc ttgaggcaaa ccatgatgcg agaacgaggc aatatgccgc tgtaaaatag
     6421 actgccccac cccccgcgag ccgctatgaa ccaacagttg cagatgttgt gcatccagac
     6481 ctgccagtgc aaacagttca gcgttgatga tttgatcaac ttgttgcagt tcagcgaaat
     6541 gattgccgcc accgatagag ccaagcgagc tgcgccacgg atgctgtgca aatgctgacg
     6601 gcaggttttc ctccagccag ctttcttcag caacgtcatc cagatctgat aatcgctttt
     6661 caaacttatc ggcgttgtat ttgcgagcga gaatatctgt ttgccatagc gccataccgc
     6721 agccgatatc attgccgacc agtgccggat aaaaacgacc aacagagaag aacgctgcgc
     6781 caatcggata gccgcgtccg ggatgtaaat ctggcatccc cactacgcgc tgcatgttgg
     6841 gtaaatttgc cgtggtgtgt aattgttgga tcgctaaact ctcgatccac aggtcatcag
     6901 atgcgatggt aaataccgca tcggataagg gacgaatata attgcccata taccattccg
     6961 taaatagact cgtcatactt caagttgcat gtgttgcgac ttgaattatt gagagtaaca
     7021 attaaaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat taatcctgga
     7081 aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt gcagtttgtg
     7141 gaataagaaa acgcgcacat actaggcttg cgttattttc attgcaagct ggatttaatg
     7201 ttgctgttta taacgcatat aaaaattaat aaaccactct ccccgacatt caacgcgttg
     7261 ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta tgcttgcgtc
     7321 caccgtaagt tcggattcag acttaatcaa aggttttaac aaagcgctgg caaccccacg
     7381 gcgggtgtat tcagggtcaa caaataacat atcgatatag tgttcaacgc aggtaataaa
     7441 gccgaccagt tttgcattaa tgactgcaac ccgcacttgt gatttcgcga gtttctcctt
     7501 ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggtgaat aatgctgact
     7561 ggcagtcatc gtaaccgctc taatgaaaat agcgcatagt tgctgaaaat cgccaggctg
     7621 atagtctctt atttgtatgt tattcataat cccagcacca gttgtctttc catttgcggg
     7681 tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg gcgttcatcc
     7741 caggttttac gcgcggtggc gattaagtca gctttattca gccgcggcca gacgtgctcc
     7801 tgagtggttt gctgaaaatc gtcgaacttt aatttcaccc cctggcgggc aatcagtaaa
     7861 tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc gataatcgct
     7921 tcacattcag accagtggtg gatatcttcc gccatcgtgc gttccacgcc gacggatttt
     7981 cgcaaccgtt cgctgttaac gtcgcgctcg tcaatcccct gactacgctc ccacaaaatg
     8041 cggccaaatt tgccaaagcg tttaagcagc atcaccagat cacacttttg tacatcaccg
     8101 caggtccgca gccccatcgc ttccagtttt gccgccgaga ctttgccgac gccggggatt
     8161 tttgccagcg gtaaggtttg taaaaatgcc ggaacttctg ccggcgtaat cacaaactga
     8221 ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttactgg tgccacgccc
     8281 gcagacgctg tcagttgcag ctcgctgaaa atcgtctggc ggatttcctg ggcgatgagg
     8341 gtcgcagaac cgtggcaatg gacgctatcg gtgacgtcga gataagcctc atccagtgac
     8401 aacggttcaa tacgcgaggt gtagcgcgag aagatttcac ggatatgatt tgaggcttct
     8461 ttgtaggcgt caaagcgccc cggaagcaag gtgagatgcg ggcataattt gagcgccatc
     8521 cctgtcggca tagcgctacg tacgccaaat ttacgcgcgg gataattggc ggtgctgatc
     8581 accccccgac gttcgcggct gccgccaata gcaataggga tatcgcgcag ggcgggattg
     8641 tcgcgcatct ccaccgctgc gaaaaagcag tccatatcca catgaatgat tttacgcatt
     8701 actcacctct caacactggt aaagtataca gtgatttcag ggtttgagaa atgcgtaaag
     8761 attcagcatt taacgcgttc gctgcgaaag cacctgctgc ttatccagct tttgcaccag
     8821 cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat cggacgcact
     8881 tttggtcagc accataatct caatgcgccg gttgcccgcg cttttcggat ttttggaatc
     8941 cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttatcttccg gcattccggc
     9001 ctcttccagc acccgacgag ccgaaagcgc gcggtcaccc gaaaggttcc agttgttgta
     9061 gatattgttt ttgtaagcca tcgcatcggt atgcccggta ataataattt tgttatcgag
     9121 cgagtcgaaa actggcgcga gctccaccag cagcgtttta aagaacggca taatctgcgc
     9181 gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta atcagcacgc gtaatccctg
     9241 gggaacaatc tccatctcca gattcgcttc catatgcgca tcacgggcaa tggtggtgat
     9301 actggttgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagcg ccgttttctc
     9361 attgacgtcg cgcgcttttt tctccgtttc ttccggcacc gccacggtgg caggttttgg
     9421 cggatgagac gggctgattt tatttaacgg cgacagccca ccaccgttaa aaatcgactg
     9481 cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctgt tgacaatcaa
     9541 ctttattgtc gtcaccaaag gggccgagcg tatctccgag gtttctgccc gctttaccct
     9601 ggacgcgatg cccggtaagc agatggcgat tgacgccgat cttaatgccg ggttgatcaa
     9661 tcaggcgcag gcacaaaccc gacgtaaaga tgttgccagc gaggccgatt tctacggcgc
     9721 gatggacggg gcatcgaaat ttgtgcgcgg ggatgctatc gccggtatga tgattctggc
     9781 gatcaacctg atcggcggcg tctgtatcgg gatcttcaaa tacaacctga gcgccgacgc
     9841 cgccttccag cagtatgtgc tgatgaccat cggcgacggg ctggtggcgc aaatcccttc
     9901 cctgctactc tccaccgcgg cggcgattat cgtcacccgc gtcagcgaca acggcgatat
     9961 cgcccatgac gtgcgtaacc agttgctggc aagcccatcg gtgctctaca ccgctaccgg
    10021 aattatgttc gtactggcgg tagtgccggg aatgccacat ctgccgtttt tgctgttcag
    10081 cgccctgctt ggctttaccg gctggcggat gagcaaacgc ccgcaggcgg cggaagcgga
    10141 agagaaaagt ctcgaaacgc tgacccgcac catcaccgaa accagcgagc aacaggtcag
    10201 ttgggaaacc attccgctga tcgagcccat cagcttaagc ctcggctaca agctggtggc
    10261 gctggtggac aaagcccagg gcaacccgct cacccagcgt attcgcggcg tgcgacaggt
    10321 gatatccgac ggcaacggcg tgctgctgcc ggagatccgc attcgggaaa acttccgcct
    10381 taagcccagc cagtacgcta ttttcatcaa cggcattaag gctgatgaag cggatattcc
    10441 ggcggataaa ctgatggcgc tgccctccag cgaaacctac ggcgagatag acggcgtgct
    10501 ggggaacgac ccggcgtacg ggatgccggt gacctggatc cagcctgcgc agaaagcgaa
    10561 ggcgctaaat atggggtatc aggtgatcga cagcgccagc gtgattgcta cccatgtaaa
    10621 taagattgtg cgcagctata ttcctgattt atttaactat gacgatatta cgcagttgca
    10681 taaccgtttg gcgtcgatgg caccgcgcct ggcggaggat ttaagcgcgg tgctcaatta
    10741 cagccagttg ctgaaagtgt accgggcgct gctgaccgaa ggcgtttccc tgcgcgatat
    10801 cgtcaccatc gccaccgtgc tggtcgccag tagcgcggtg accaaagatc atattctgct
    10861 ggcagccgat gtgcgcctgg cgctgcggcg cagcattacc catccgttcg ttcgcaagca
    10921 ggagctgacg gtgtataccc tgaataatga gctggaaaat ctgctgacta acctagtgaa
    10981 tcaggcgcaa cagggcggta aagtgatgct cgacagcgtg ccagtggacc cgaatatgct
    11041 caaccagttc cagagcacga tgccacaggt gaaagagcag atgaaagcgg cggggaaaga
    11101 cccggtgctg ctggtgccac cgcagctgcg ccctttgctg gcgcgttatg caaggttgtt
    11161 tgcgccgggg ctgcatgtgc tgtcgtataa cgaagtgccg gatgagctgg agttgaagat
    11221 tatgggggcg ttgagttgat gggggattct tttccatgct ttgcagcacc tgacaccatt
    11281 aattgacgtc acactgcaaa gagactacga tgacaccata caatgacgtc atggattgat
    11341 agtatgatta aggtcagcgc gctaggttct cgtcatagaa aaacattcga cgatgtaatg
    11401 acggagccgc caaaatctgg aattaaatgg gatgatgttg ttgcactcat taaagctgtg
    11461 ggtggaacaa taaaaaataa caacggctcc agacgaaaat ttcagattgg aacgaccaaa
    11521 ttcagcacac atgagccaca tccaaagaat gtgatggata aaggcgcagt agcaggtctt
    11581 aaggagtggt ttgttaactg tgtaggggta gattatgaat aaagcatcga acattatcac
    11641 aattaaaggt caaccagccg cagtgacatt tgaagccgat atcaacgcat ttcgcggcaa
    11701 gttcctcaat gtaaacggtt attgtgactt tgtagccaca agtattgagg cattatatcg
    11761 tgaaggcgaa agcgcgctgg atgagtggat ggcggattgt gaagaggacg gtattgcccc
    11821 ttttcgtgaa gaagaagagc aaaaacgcct gacgcttcgt gttcctcatc acattgattc
    11881 gcggctgacc atcgtcgcca gacaacattc catttcaaaa aaccagctca ttgttgatgt
    11941 cttagaacgt gagttttcag cccagatgta aacaggtcgg atgccgcgtg gcatccgacc
    12001 ttttgccagt tcgtcaatat tacccattta caaaatcgtc tcttccgtca atatccgccg
    12061 cgcgcctaaa aagtgatcct gccagaaagg ttcagctaac cgacttaccc gaatggtttc
    12121 cccggtgcgc ggtgactcaa taaactgacc gtcgcccaga tacaccccca tatggtcggc
    12181 aatctcgcgg ctgtggatat ggaaaaatag caaatctccc cggcgcaggt cgttattcgc
    12241 cacaatggtt gcccggcgat agtgatacat ctcattagcc gtgcgtggga gtttcgcctc
    12301 aaggatcttg ttgtaggcat aaaaaactaa cccgctacag tcgaagcctt gatcgggccg
    12361 cgtaccgccc cagacgtaag gcttgccaag ctgttgctcc agacggtgaa tcgccacttc
    12421 ggtgatgtta tgcagatggt cgctgcttaa gaaatggttg ttttccgcca gcgcctgcca
    12481 gcgtctgtca ctcgccttga gcggcgcagg aaaccattct ggatgctgtt ttatctgttc
    12541 gcggttgtgc tggcgtagcg cccttctgct ttcggcattg ccttccacaa tatagctggc
    12601 ctgttttttc agatgggttt ggtactgttt taataaacgc gcacgatttt gcatccgctg
    12661 gcgggcggcg tagctgaaag tgatatgtga tgtggtggcg ctggcaaatg aagaaaacgg
    12721 caagcaaagc aacagtaatc ccgacaacac aaaagaggga atcgacggca aggacatact
    12781 caataatggc tgatcatgca ttgatgttgc agtgtatccc ggcccgtacc tgaacaaact
    12841 taataaacca ttattataaa attaataaat atatttcagt taattatgta aagacatccg
    12901 tttattaaat tactcatgga ttgtcgctgt tgctcatttg agctacaatt caagctgaat
    12961 aaatatacag cacaggagat accccaatgg ctgctaacgc atttgttcgc gcccgaatcg
    13021 atgaagatct gaagaatcag gcggcggacg tactggccgg gatggggctg accatctctg
    13081 acctggttcg cataaccctc acaaaggtcg cgcgtgaaaa ggcattgccg tttgatttac
    13141 gcgagcctaa tcaattaacc attcaatcaa tcaaaaacag cgaagctggc gttgatgttc
    13201 ataaggccaa agacgccgat gatttatttg ataaattagg aatttaaata tgattcaaag
    13261 ggatattgaa tactcgggac aattttcaaa ggatgtaaaa cttgcacaaa agcgtcataa
    13321 ggatatgaat aaattgaaat atcttatgac gcttcttatc aataatgctt taccgcttcc
    13381 agctgtttat aaagaccacc cgctgcaagg ttcatggaaa ggttatcgcg atgctcatgt
    13441 cgaaccggac tggatcctga tttacaaact taccgataaa cttttacgat ttgagagaac
    13501 tggaactcac gcggcgctct ttgggtaaag cacgctgccg atgaaatttg taaaccctgc
    13561 gccgacaggc ttgcgtaaaa ctcttttggt gataatgtga gccttccaaa tccagactat
    13621 tccgatttgg gatctatttt gccgtcctgg cgtgtgtaac cgttttatca aggaataagc
    13681 agtatgcgta aaatcgcatt aattcttgcg atgttgttga tcccgtgcgt ttcgtttgcc
    13741 ggtttgctgg gcagcagtag ctcgaccacg ccagtgagca aagagtataa gcagcagttg
    13801 atgggatccc ctgtctacat ccagatcttc aaggaagaac gtacgctcga tctctacgtc
    13861 aaaatgggcg agcaatatca gctgctcgac agttataaaa tctgtaaata ttccggcggc
    13921 ttagggccaa aacagcgtca gggcgatttc aaaagcccgg aagggtttta tagcgtccag
    13981 cgtaatcagt taaaaccaga cagccgttac tacaaagcga ttaatattgg tttccccaat
    14041 gcctatgacc gtgcgcatgg ttatgaaggg aaatacctga tgatccacgg cgattgtgtt
    14101 tccatcggct gctacgcaat gaccaatcag ggtattgatg agatcttcca gttcgttact
    14161 ggtgcgctgg tgtttggtca gccgagcgtg caagtgagta tttacccgtt ccgcatgacc
    14221 gacgccaata tgaagcgcca taaatattcc aactttaagg acttctggga gcagctgaag
    14281 ccgggctacg actactttga gcaaacccgt aagccaccga ccgtttctgt ggtcaatggc
    14341 cgttacgtgg tcagcaagcc gttaagccac gaagttgtgc agccacaact ggcatcaaac
    14401 tacacgctcc ccgaggcaaa ataagcgcca ttcgcctgac ataatctttt gccaggtttc
    14461 attgcccgtc agcggctgtg ttgcaatcac cgtgaccaca tcattcggtg tggtctgcga
    14521 gctgaagtcg atttccacat cctgatccag cagcgttgcc acgccaaacg gcgcgcggcg
    14581 ggtgatccag tgtaaattag tcgagcaata cgccatcaca tagcgcccgt cagaaagcag
    14641 catgttgaaa acgcccttct gccgcagttc atccgccagt gaggcgatat atttaaacac
    14701 tgccgccatg ttgcccggtg tgcgcgggta acgctgcgtt aatttatgca ggagccagca
    14761 gaaggctttt tcgctatcgg tttcaccgac cgggcggaag ttgccggttt ccagtgattt
    14821 gtagcccgtc agttgtccgt tatgggcata agtccagtta cgcccccata actcgcgggt
    14881 aaatgggtga gtattttcca gcgccacctc gccccgatta gcctggcgaa tatgagccac
    14941 caccgaacag gattttatcg ggtagtcctg gacaagtttg gcgatggggg aattaaagct
    15001 gggttgtgga tctttaaatg tgcgacagcc tttaccttcg taaaaggtaa tgccccagcc
    15061 atctttatgt ggcccggttc caccaccacg ctgtacaagt ccggtgaaac taaagcagat
    15121 atcggtaggg acgttggcgc tcatcccgag caattcgcac atgggtaacc tccacaacag
    15181 cgggagcgaa tggcccccgg cgaagattga tttaagaatt ttcaggtcgg atgcggcgta
    15241 aacgaattat tcggcctacg gttcgggtac agatttgtag gcatgataag acgcgtcagc
    15301 atcgcatcag gcatcagcgc acaagtgccg gatgcggcgt aaacgcctta tccagcctac
    15361 gccagactta cttaaccatc tctttttcaa ttaactggat caggatatgg atcactttaa
    15421 tgtgaatctc ctgaatgcgg tcggcataac caaagtgcgg tacgcgaatt tcgatatccg
    15481 ccgtgccagc cattttgccg ccgtctttac cggtcagggt gatcactttc atccccttct
    15541 cacgcgccgc tgcgatcgct ttgatcacgt ttgcagagtt accggaggtg gagatcccca
    15601 gcagtacatc gccttcgcga cctaccgctt caacgtagcg ggagaaaata tcattgaaac
    15661 cgaaatcatt accgacgcag gaaatatgac taacgtcaga aatagcaatc gccgggtagc
    15721 ccggacggtt ttcacggtag cgaccggtca actcttcggc aaagtgcata gcgtcgcaat
    15781 gggaaccgcc gttgccgcag gaaagcactt tgccaccggc tttaaagctg tctgctaaca
    15841 ggaccgccgc gcgctgaatg gcgtgaatat tggcgtcatc ttttaaaaag ttagccagcg
    15901 tttccgccgc ttcgttcagt tcgttacgaa taagatcctg gtacatgagg atatccttca
    15961 gcataaatgt aatagacaaa atgcagtgta ccggataccg ccaaaagcga gaagtacggg
    16021 caggtgctat gaccaggact ttttgacctg aagtgcggat aaaaacagca acaatgtgag
    16081 ctttgttgta attatattgt aaacacattg ctaaatgttt ttacatccac tacaaccata
    16141 tcatcacaag tggtcagacc tcctacaagt aaggggcttt tcgttatgat gattttgagt
    16201 attctcgcta cggttgtcct gctcggcgcg ttgttctatc accgcgtgag cttatttatc
    16261 agcagtctga ttttgctcgc ctggacagcc gccctcggcg ttgctggtct gtggtcggcg
    16321 tgggtactgg tgcctctggc cattatcctc gtgccattta actttgcgcc tatgcgtaag
    16381 tcgatgattt ccgcgccggt atttcgcggt ttccgtaagg tgatgccgcc gatgtcgcgc
    16441 actgagaaag aagcgattga tgcgggcacc acctggtggg agggcgactt gttccagggc
    16501 aagccggact ggaaaaagct gcataactat ccgcagccgc gcctgaccgc cgaagagcaa
    16561 gcgtttctcg acggcccggt agaagaagcc tgccggatgg cgaatgattt ccagatcacc
    16621 catgagctgg cggatctgcc gccggagttg tgggcgtacc ttaaagagca tcgtttcttc
    16681 gcgatgatca tcaaaaaaga gtacggcggg ctggagttct cggcttatgc ccagtctcgc
    16741 gtgctgcaaa aactctccgg cgtgagcggg atcctggcga ttaccgtcgg cgtgccaaac
    16801 tcattaggcc cgggcgaact gttgcaacat tacggcactg acgagcagaa agatcactat
    16861 ctgccgcgtc tggcgcgtgg tcaggagatc ccctgctttg cactgaccag cccggaagcg
    16921 ggttccgatg cgggcgcgat tccggacacc gggattgtct gcatgggcga atggcagggc
    16981 cagcaggtgc tggggatgcg tctgacctgg aacaaacgct acattacgct ggcaccgatt
    17041 gcgactgtgc ttgggctggc gtttaaactc tccgacccgg aaaagctgct gggtggcgca
    17101 gaagatttag gtattacctg tgcgctgatc ccgaccacca cgccgggcgt ggaaattggt
    17161 cgtcgccact tcccgctgaa cgtaccgttc cagaacggac cgacgcgcgg taaagatgtc
    17221 ttcgtaccga tcgattacat catcggtggg ccgaaaatgg ccgggcaagg ctggcggatg
    17281 ctggtggagt gcctctcggt aggccgcggc atcaccctgc cttccaactc aaccggcggc
    17341 gtgaaatcgg tagcgctggc aacgggcgcg tatgctcaca ttcgccgtca gttcaaaatc
    17401 tctatcggta agatggaagg gattgaagag ccgctggcgc gtattgccgg taatgcctac
    17461 gtgatggatg ctgcggcatc gctgattacc tacggcatta tgctcggcga aaaacctgcc
    17521 gtgctgtcgg ctatcgttaa gtatcactgt acccaccgcg ggcagcagtc gattattgat
    17581 gcgatggata tcactggtgg caaaggcatt atgctcgggc agagcaactt cctggcgcgt
    17641 gcttaccagg gcgcaccgat tgccatcacc gttgaagggg ccaacattct gacccgcagc
    17701 atgatgatct tcggacaagg agcgattcgt tgccatccgt acgtgctgga agagatggaa
    17761 gcagcgaaga acaatgacgt caacgcgttc gataaactgt tgttcaaaca tatcggtcac
    17821 gtcggtagca acaaagttcg cagcttctgg ctgggcctga cgcgcggttt aaccagcagc
    17881 acgccaaccg gcgatgccac taaacgctac tatcagcacc tgaaccgcct gagcgccaac
    17941 ctcgccctgc tttctgatgt ctcgatggct gtgctgggcg gcagcctgaa acgtcgtgag
    18001 cgcatctcgg cacgtctggg ggatatttta agccagcttt acctcgcctc tgccgttctg
    18061 aagcgttatg acgacgaagg ccgtaatgaa gccgacctgc cgctggtgca ctggggcgta
    18121 caagatgcgc tgtatcaggc tgaacaggcg atggatgatt tactgcaaaa cttcccgaac
    18181 cgcgtggttg ccgggctgct gaatgtggtg atcttcccga ccggacgtca ttatctggca
    18241 ccttctgaca agctggatca taaagtggcg aagattttac aagtgccgaa cgccacccgt
    18301 tcccgcattg gtcgcggtca gtacctgacg ccgagcgagc ataatccggt tggcttgctg
    18361 gaagaggcgc tggtggatgt gattgccgcc gacccaattc atcagcggat ctgtaaagag
    18421 ctgggtaaaa acctgccgtt tacccgtctg gatgaactgg cgcacaacgc gctggcgaag
    18481 gggctgattg ataaagatga agccgctatt ctggtgaaag ctgaagaaag ccgtctgcgc
    18541 agtattaacg ttgatgactt tgatccggaa gagctggcga cgaagccggt aaagttgccg
    18601 gagaaagtgc ggaaagttga agccgcgtaa ttgaataacg gagccgaaag gctccgtttc
    18661 tttatccgct aattatttaa aattaaagcc atccggatgg ttttccaggc tgccggtcaa
    18721 cgccgcgaac agcaccgttt taccatcaat cgaaagcgca tcgttcacat tcagccaggt
    18781 gagtttctct tgcgacgttt tctcatcaat agtcgagaac agccccgtca tctgattaga
    18841 tttctcggac cacatcacag cgatacgttg cgagccacag tcatgcggtt tgcacgcgct
    18901 catcacctga tacgtctcat ctcccaacgt tacggtttgt gcgggagtat aagtaccgcc
    18961 tttcatcacc caggcaggca gcttatgccc ttgtaccatc tgattaaatg cagctttggt
    19021 ggtttcgccc tttgcaaggc tgctaatggt taaatcatcc tgcgccattg cactggtggc
    19081 gatgaccaga gcggcgactg tcgttattgc cttaaacatc attcctcccg agcttatcct
    19141 gcccatgtta ttaaccttct aaagcctggc gataacacga taatttgcaa atcagatatt
    19201 tccaatatca gcatttgtta tcaatttatt ctgcgctaag gcaactgcct gataaggtgt
    19261 taaagttata aaacccttcg cataaaggag ttgtacaccg tgcctggttt gaagattacg
    19321 cttttgcagc aaccactggt gtggatggat ggccctgcca acctgcgtca ttttgatcgt
    19381 caactggaag gtattaccgg gcgcgatgtg atcgttctac cggagatgtt taccagcggc
    19441 tttgccatgg aagcggcagc ttcgtcgcta gcacaagatg acgtagtgaa ctggatgaca
    19501 gctaaggcgc agcagtgcaa tgcgctgatt gcaggcagtg ttgcattaca aacggagtct
    19561 ggttcggtta accgcttttt gctggttgag ccgggcggca cggtacattt ttatgataag
    19621 cgtcatctgt tccgcatggc agatgagcat ctacattata aagcgggcaa tgcgcgagtg
    19681 attgtggaat ggcgcggctg gcgtattttg ccgctggtgt gctacgactt acgttttcct
    19741 gtgtggtcgc gcaatctcaa cgattatgac ctcgccctgt acgtcgccaa ctggcctgct
    19801 ccgcgctctc tgcactggca ggcattgctg acggcccgcg cgattgagaa tcaggcgtat
    19861 gtggcgggat gcaatcgcgt cggcagcgat ggcaacggct gccattatcg cggtgacagc
    19921 cgggtgatta atccacaagg cgagattatc gctactgccg acgcgcatca ggcaacgcgc
    19981 attgatgccg agctgtcgat ggcggtgtta agggaatatc gcgataagtt tccggcatgg
    20041 cgggatgcgg atgagtttag gttgtagtga gtgggggctg tatttagggc ggggaattca
    20101 gaaagggatg tttagtgtgc tgagcgagag ttaaataata aaaaactggg aagatttaat
    20161 aatccccccg cagggcaaga gcatgaatta aatttgtgtt aaatttttca ttttgtcagt
    20221 tcgaaaccca agctctgagc aggatgcaag cacttcttct atataacatg tgtttaacgc
    20281 cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag tttctgtcca
    20341 tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa
    20401 tattaatagc aatgtgccgt atccctgaaa ataattctgc tgcatttcct cttcttattt
    20461 tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc tccaagtgcc
    20521 agtgatttcg gatcgctgtg gcgaatttct cagcggttaa atcagcagaa ctgatataat
    20581 atctgaccgt catttctggc tctttctttt gttctgctat tatggaccga aaggagactg
    20641 ccccacataa tttcttcagc cctttccatt caaacgtgaa atcaataagt tcatcaggga
    20701 catcgcaaac aatatgaaga cggatttctt ctctgccgtg actcttttca ctaattgcgt
    20761 aactgtcatg ctctggatta tttaattctt tcagcggaaa tttttcctca aaggctttat
    20821 ttagccgccc ctggtttcct tttaccgcga ataaataatc acctccctgt ttttgtatct
    20881 tctctgcaat atctttctgg caacccatcg catcagttgt gatgattttt cctttaatat
    20941 ccagcatgtt aagaagttct gggatagctg taatctcatt agatttctca tccgtcctga
    21001 tctgtccgat gaccagactg tgcattgttg agaacgcact aatgacatga atcgctcccc
    21061 tgcggcgact cttgtcataa gagtgccgga gcgtttttcc atcaattgcg atgacgtctt
    21121 tatcatctga agaatggcag tcacgcatcc agttaataaa gcactcatga aattttgcag
    21181 gactgataca ggatacaact ctggcaatag tatcgtgaac aggaatacca ttttcaaaat
    21241 caccatattg ct
//
LOCUS       DAZFYC010000079        21120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFYC010000079 DAZFYC010000000
VERSION     DAZFYC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.079"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            171..680
                     /gene="tssB"
                     /locus_tag="ACHKW5_003320"
     CDS             171..680
                     /gene="tssB"
                     /locus_tag="ACHKW5_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7512659.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGSGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKL
                     RRRRTFNPK"
     gene            729..2267
                     /gene="tssC"
                     /locus_tag="ACHKW5_003321"
     CDS             729..2267
                     /gene="tssC"
                     /locus_tag="ACHKW5_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7512660.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGENVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFSNLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWVRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2285..3622
                     /gene="tssK"
                     /locus_tag="ACHKW5_003322"
     CDS             2285..3622
                     /gene="tssK"
                     /locus_tag="ACHKW5_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7512661.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALMSGKLKTQTVRLRFADGTLIDSDVSDVLPLACDLHALTNDSAVVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDEGYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLGSLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3619..4284
                     /gene="tssL"
                     /locus_tag="ACHKW5_003323"
     CDS             3619..4284
                     /gene="tssL"
                     /locus_tag="ACHKW5_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO7512662.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     GVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKDPLQAHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4297..5949
                     /locus_tag="ACHKW5_003324"
     CDS             4297..5949
                     /locus_tag="ACHKW5_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7512663.1"
                     /translation="MRNTLKQAVVLWGMVLLLVLWSVFISPSGVLIWAGAAAIVLTVA
                     ALLIYRRRQAWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVSDEEQLVAQAERLLTLRPAWASQLAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEIHVATSAETASPAQWLTQTSTQE
                     RSQQLCYLLKAESLMQWLNLNMLAALNGPETKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDATLPFPDALLRRLPRQSGFTPLRRACVTMLGITTVAGIAALC
                     LSATENRQLLRHIGDDLHQFYAVPAEEFITKARRLSVLKDDAIMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            6007..6498
                     /locus_tag="ACHKW5_003325"
     CDS             6007..6498
                     /locus_tag="ACHKW5_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7512664.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFIFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKEPSKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6689..9334
                     /gene="tssH"
                     /locus_tag="ACHKW5_003326"
                     /gene_synonym="clpV"
     CDS             6689..9334
                     /gene="tssH"
                     /locus_tag="ACHKW5_003326"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO7512665.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLTALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQATEPLTSAQPESATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTASLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLQARLTALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKRL
                     AQQIMALRQEGADSTDLQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVVR
                     RLRQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9346..11853
                     /locus_tag="ACHKW5_003327"
     CDS             9346..11853
                     /locus_tag="ACHKW5_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7512666.1"
                     /translation="MGIQNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLARSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMAP
                     AMAILDGAQSQMQSLSTDAQTTNADPADLSSQIALLQQSVKDLTQAVILLSAPKGVAI
                     ASGEHLQLAASKNVIANAGNNADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKM
                     SGITDENGNTELISSDKEEVVNISVFEPDEFLDDDIN"
     gene            11875..12468
                     /locus_tag="ACHKW5_003328"
     CDS             11875..12468
                     /locus_tag="ACHKW5_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7512667.1"
                     /translation="MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAI
                     RLKQIRQLVRNNNKSIMGEDYIICQIYKESRFKQFAGKNKHNAKGLMQMQRNAVRQVF
                     KYRQQKIKGRMTTDKETNEAFANADTFYKSDKIFDEKENIKIGTEYLQYWIDKEATIE
                     EAYRGTDEAYYSVIKPCAEKLAKDPDNIQILMEGIGR"
     gene            12572..13021
                     /locus_tag="ACHKW5_003329"
     CDS             12572..13021
                     /locus_tag="ACHKW5_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512668.1"
                     /translation="MQLLSLRTIDGDTLYLKFRNVITEAFLDTPTKSYDFFGDVVLSQ
                     CINGSLIFALEYGSPYIKGCLVTGWENGFKGKNSPEGFCFAERNIPEAVWFGKNNILI
                     IIRNQMDVGTWKGKYIIYDNAKKSGERAYSSDNLPSKDGYMIFALNK"
     gene            13099..14313
                     /locus_tag="ACHKW5_003330"
     CDS             13099..14313
                     /locus_tag="ACHKW5_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512669.1"
                     /translation="MPVRLNTIPDAESYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPHFWGLACGVPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVYLEPKTPERTAESSTALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGIWTAELDREQRTALLSL
                     NQPFTQEALMYELDAFLGRSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            14370..17696
                     /locus_tag="ACHKW5_003331"
     CDS             14370..17696
                     /locus_tag="ACHKW5_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7512670.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKK
                     LRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQA
                     GRPLQSVGCLLPERCTPEQLTAMLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAER
                     GIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSQVWAGVTGDNAR
                     GRAVGFPWLRAALMSAVCVLAIWGAGMTTSFFANCALVQETGIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADSLRY
                     ENIPEGVQQSVFPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARHEEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFTGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELQAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            17689..19299
                     /gene="tssA"
                     /locus_tag="ACHKW5_003332"
     CDS             17689..19299
                     /gene="tssA"
                     /locus_tag="ACHKW5_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7512671.1"
                     /translation="MSELLQKLTRSCFVDRDALDVARTQAALWQTWLLPVTANTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALSQLTVSFTAWPEDQHSPSLMLLINALESRLAQSGGMNAVVPQNSSSVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDDTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            19305..20723
                     /locus_tag="ACHKW5_003333"
     CDS             19305..20723
                     /locus_tag="ACHKW5_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7512672.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIGGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGGSEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSEQQTGKYFDKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLHNSPEEQKWVDVKVDRKQKTVDIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVNLTKNGVEFYWSRNGSRGGGIGENIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            20716..>21120
                     /locus_tag="ACHKW5_003334"
     CDS             20716..>21120
                     /locus_tag="ACHKW5_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512673.1"
                     /translation="MTRVVIALLFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTVNKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDS"
BASE COUNT         5133 a         5531 c         6011 g         4445 t
ORIGIN      
        1 aagaggctaa ataatatcta aaatgataca accgtatcta ttccccagaa aaatacatta
       61 ttcattttta tattttccta atcattgctt tttatttaaa tcacccgata atcccgtgta
      121 tataattatg tcaataacca tcagaaaaag tggatgatga ggaaaaggat atggccgaca
      181 gtttccagaa tgaagttccc gccgctcgtg taaatatcaa gcttgacctg catacaggca
      241 atgctaaaaa gaaagttgaa ctccccctca agcttcttgc tgttggcgat tacagtaacg
      301 gaaaagagca acgtccgctg tccgaacggg acaagattga tatcaataaa aacaacttca
      361 acagtgtcat ggctgagttt tcgcctgcgg ttaatttaac agtagaagac acactaagcg
      421 gaagcggtaa tgagcaaaat atcgcgcttg aatttaaaag cctgaaagat tttgaacccg
      481 aacaggtagc aaaaaatatc ccacagcttc gtgtattact cgctatgcgc aatttattac
      541 gtgacctgaa atccaatctg ctggataacg caacgtttcg ccgtgaactg gaaaatattc
      601 tgaaagaccc aacgctcagt tcagaactgc gtgatgaact ggcgaaattg cgccgcagga
      661 gaacgtttaa cccgaagtga tattcaggtt aaaccggaga agaaaggatc actgaaaagg
      721 aaatgctgat gtctgtacaa caagaacatg ccacctctga aactgcaaca ctgaccacca
      781 ctgagtccgg cggcgtttat cagtccctgt ttgataaaat taatttaacc ccggtgtctt
      841 ccattcagga aatagattta tggcaaaaca gcgaaacact ggcggatgcc tcacccgatg
      901 agcgcgtgac agcggcgatt cacgttctgc tttcctgtct ggcgaaatca ggcgagaacg
      961 tggtcaagct cgacaagagc ctgctggatt ttcatatcga cgatttggat cagaaaatca
     1021 gtaagcagct tgatgcggtc atgcaccatc ctgaattcca gaaagtcgag tcgctgtggc
     1081 gcggcacatg gttcgtcgta cagcgcactg attttcgcaa aaatgtcaga attgaattgc
     1141 tggatatcag caaagagcat ctgcgtcagg atttcgacga ctccccggaa atcattcaga
     1201 gtggtttata tcgtcataca tatattcagg agtacgatac gccgggtggc gaacctgttg
     1261 cctcattaat ttccagctat gaatttgata acagcccgca ggatattgcc ctgctgcgca
     1321 atatttccag agtgtctgcc gcttcccata tgccttttat cggttctgtc gggccgaaat
     1381 tcttccttaa aaattcgatg gaagaagtcg ccgcgattaa agatatcggc aactactttg
     1441 accgcgcaga atatattaaa tggaaatcgt ttcgtgatac cgatgacagc cgctatgtgg
     1501 gattagtgat gccgcgagtg ctgggccgtc tgccctatgg gccggacacg gtgccggtac
     1561 gcagctttaa ctatgtggaa gaagtcaaag gcccggatca cgaaaagtat ctctggacaa
     1621 acgcctcgtt cgcctttgcc gccaatatgg tgaagagctt tgtgaataat ggctggtgcg
     1681 tgcagatccg cggtccacaa gcaggtggcg cagtggccga tctgcccatc catctttacg
     1741 atctcggcac cggcaatcag gtcaaaattc cgtccgaagt gatgatcccg gaaacccgcg
     1801 aatttgaatt ttccaacctt ggctttattc cgctctctta ttataagaat cgcgattacg
     1861 cctgcttctt ctcggcaaac tctgcccaga aaccggcgtt gtatgatacc gctgacgcca
     1921 ccgccaacag ccgtattaac gcccgtctgc cctatatctt cctgctgtcc cgcattgcgc
     1981 attacctgaa aatcatccag cgcgagaata tcggtaccac caaagaccgc cgcgtactgg
     2041 aactggaact gaacacctgg gtccgcacac tggtgacgga gatgaccgat ccgggtgatg
     2101 aactgcaggc gtctcacccg ctgcgcgacg gtaaggttat cgtggaagat attgaggaca
     2161 atccgggctt cttccgcgtc agactctttg ccgtgccgca tttccagatt gaagggatgg
     2221 atatcaacct gtcactggtt tcccagatgc cgaaagcgaa agcctgaccg ggaagtggtg
     2281 tgatatgaaa attcatcgac cactgtgggc tgaggggaca ttcctgtcct ctcagcagtt
     2341 tcagcagcag gcccgctggg aagcgttcag caatgacagc attgcgcaac tttgtattcg
     2401 tcatccgtgg gggatcgcga atgtgctctt tgaccgcgat gccctgatgt ccggaaagct
     2461 gaaaacgcag acagtacgcc tgcgttttgc tgatggcacg ctgatcgatt ctgacgtgag
     2521 cgatgttctg cctctcgcct gcgatctgca cgcgctgacg aacgacagcg cggttgttct
     2581 gctggcgctg ccgcttgccc atggtaacgg cggtaatctc ggtcagggtg agcagactga
     2641 aagaccgcta cgctatcgtc aggaatggca aaaggtacag gatatctacg gcagtgacag
     2701 tgaagatatg gccgttgagc gtcatgcgct gtccctgcgc tttgctcatg ataacaatca
     2761 ggattacatc acctgcccac tggcgcgtct ggtgcgggat gttcagggca actggacaca
     2821 ggatgagggc tatattccgc cgttactggc gtttaatgct catgacgggt tggtacagcg
     2881 tctggacacg ctgctgttgc agctacgggc caaatgtcag cgcctgatgg cgatgcgtcg
     2941 ggaaagcaac cagcgtatgg cggacttcgc cgtcgccgat gtctccctgt tctggttgct
     3001 taacgcgctg aactccgccg agccggtgct gagtgatttc ctgcgctacc cggcggttca
     3061 tcccgaactg gtctggcggg agctggcgag gcttgccgga gcgctgctga cgttttccct
     3121 tgaacataac gtcagcgccg ttccgcccta tgtccacgaa tcgccgtcca ctgtctttcc
     3181 gccactgttc tccctgctca gcgaactgct ggaagcaagc ctgccgtcac gggtgatcgc
     3241 gctggatctg ggatccctgc cgggcaaccg ctggaaagcc gacctgcacg atccgcgcct
     3301 gcgggaagag gccgattttt acctgtccgt tcgttcctca ctgcctgcgc atcaggtact
     3361 gcaccagcta ccgctggtct gcaaaatcgg tgcgcctgat gacgtcacgc tgcttatcaa
     3421 cgtggcgctg aatggtgttc cactggtgcc actgacctca gttccggcag cgctgccact
     3481 gcgcctggag aatcagtatt ttgcgctgga tatgcacagt gacgcggcta aatcgatgct
     3541 ggagtccggg tgctgcatga tttatgcgcc aggaaccatg ggcgacctga aacctgaact
     3601 gtttgcggtg ctgcgcacat gaaaaaagag acggatatca atatcgacgc gctgctgcgc
     3661 gacacgtttc tgacggtggt ggaactgcgt cagggaacca ccgtgcgtca cggtatggag
     3721 ctgtatcgcc actgccagcg gcaggtggag ggggtccgcg aacgcctgaa agatgccggg
     3781 ttcagccggg agaacgtgga gcacattact tacgcacagt gcgccctgct ggacgaaaca
     3841 gtcctgagtc gcagcggcat ggacgacggt caggccatct ggatgaagga tccactccag
     3901 gcgcatttct tcaacaccct gcaggcaggc gagctgctgt acgagcggat gaagcaggtg
     3961 ttgcaggagc ccgcgcctgc acaggcagtg ctgacctgct ttcaccgtgt cctgctgctg
     4021 ggatttcgcg ggcgttatca ggatccggtg gccccggagc gtgaccaact tatttcgacg
     4081 ctcaacgggc tggtcgcgcc atttggcgtg ctgccggaaa cggcggtact gaacgtgccg
     4141 ttatcccccc gtcagcaccc tttgctgcat tcgccgtttt tctggctggt aacgctgacg
     4201 ctgctactgg ccggtgtctg gtggggactg caccactggc tgaacgtgct ggtggatgaa
     4261 ttgctgccgc aaagcctgcg gtaaagggag tcgtgagtga ggaacacgct gaaacaggcc
     4321 gtcgtgctgt ggggaatggt gttactgctg gtgctgtggt cggtgtttat cagtccgtct
     4381 ggcgtactga tatgggccgg ggcggcggct atcgttctga cggttgccgc gttgctgatt
     4441 taccggcgca ggcaggcgtg gacggagatg accggcgatg ccgggttgtt atcgctgccg
     4501 ccggaaacgt accgacaacc ggtagtgctg gtctgtggcg atatgtcggc gcatctgttc
     4561 actgacagcc cggtccggca ggtttcagaa gggctgtatc tgcatgtttc tgatgaagaa
     4621 cagcttgtgg cgcaggcgga gcgattactg acccttcgcc cggcgtgggc atcgcagctt
     4681 gccgtggcat ataccgtcat gcccggcatg taccgggatg cggcggttct gaccggacaa
     4741 ctgcgccggt tcgcccacag tatggcgaca gtgcgtcgcc gggcgggcgt aaacgtcccc
     4801 tggcttctct ggagcgggct gtccggctcg ccgttgccgg agagagcgaa ttcaccgtgg
     4861 tttatctgta ccggcggcga aattcatgta gcgacgtctg ctgaaaccgc ctcccccgcg
     4921 cagtggctca cgcagaccag cacacaggag cgcagccagc aactctgtta cctgctgaaa
     4981 gccgaaagcc tgatgcagtg gctgaatctt aatatgctgg cggcactgaa cggcccggaa
     5041 acgaaatgtc cgccactggc gatggccgtg gggctggtcc cctcgttgcc tgcggtggat
     5101 aacaacctgt ggcagttgtg gatcaccgcc agaaccggcc tgacgacgga tatcgcggac
     5161 accggcacag acgccacgct gccgtttccg gatgccctgt tacggcggtt gccgcgtcag
     5221 tcgggcttta ccccgctgcg acgagcctgc gtgaccatgc tgggcatcac caccgtggcg
     5281 ggtatcgccg cgctgtgtct gtcagccacg gagaatcgcc agttattacg acatatcggt
     5341 gatgatctgc accagtttta tgccgttccg gcggaggaat ttatcaccaa agcccgtcgt
     5401 ctgtcggtgc tgaaagacga tgcgatcatg ctcgatgggt attaccggga aggagagcca
     5461 ctgcgtctcg gtctggggtt ataccccggt gaacaaatcc gccagccggt attacgcgcc
     5521 attcgtgact ggcgtccgcc tgagcaaaaa atggaagtga cagcctcgct tcaggcgcag
     5581 accgtgcgtc ttgacagtat gtcgctgttt gacgtcggac aggcccgcct gaaagacggc
     5641 tcgacaaaag tgctggtgga cgcactggtg aacatccggg caaaaccggg ctggctgatc
     5701 ctcgtggccg gatataccga cgccaccggc gacgaaaaaa gcaatcagca gttatcgctg
     5761 cgacgtgccg aagcggtgcg caactggatg ctgcagacca gcgacatccc ggccacctgt
     5821 tttgccgtac agggactggg cgagagccag cctgcggcga ccaacgatac gccacagggc
     5881 cgggcagtca accggcgcgt cgaaatcagt cttgttccgc gttctgacgc ctgtcaggac
     5941 gtgaaataaa acataccgcc ggaagaaggc ggtgcttcaa tcacactaac aaggagagta
     6001 attctcatgg ctattcctgc ttatctctgg ctgaaagatg acggcggcgc ggatatcaaa
     6061 ggttccgtgg acgttcaggg gcgcgaaggc agcatcgaag tggtggcgct ggatcacgat
     6121 gtgtacatcc cgaccgacaa caacaccggc aaactgaccg gtactcgcac ccataaaccg
     6181 ttcattttta ccaaagaaat cgatgcgtcc agcccgtatc tctacaaagc tgtgaccacc
     6241 ggtcagaccc tgaaaacggc ggaatttaag ttttaccgca tcaacgacgc cggtcaggaa
     6301 gtggagtact tcaacatcac gctggataac gtcaagctgg tcagagtcgc tccgctgatg
     6361 cacgacatca aggagccttc caaagagaag cataaccacc tggaacgcat tgagttccgc
     6421 tacgaaaaaa tcacctggac ttacaaagac ggcaacatca ttcattccga ctcgtggaat
     6481 gaacgtcctt ccgcctgata actgcggcag acaggaaact gtctgccttt ctgttgtgtg
     6541 ctttgcccag tgccgcaggc accggtactg agcaaagcat acccacaatc tggtgatacc
     6601 tgatgggcct gagtgaggta acggctatgg gcggtagcgt acctgaacgc agatcgcagg
     6661 aatacagaac acgataagga aaccttctat gaccggaaat caccccgccg cgctgctgcg
     6721 tcgccttaac ccgtactgtg cacgggcgct ggacgctgcc gcctcactgt gtcagacccg
     6781 cgcccatgcg gagataacca ttgaacactg gctgctgaaa ctgctggagc agggagaagg
     6841 cgatatcacg gtgattgccc gccgctatga atgggatatc gacacgctct ggcagtctct
     6901 gctggcacat ctggacacct taccccgctc ggtccgcgaa cgtccgcagc tttctgaacc
     6961 cctgacggcg cttatcaggc aggcgtggct gattgcctcg ctggaaggcg acgacccgca
     7021 aatccgcagt cagcacctgc tgatggcgct gacagaaaaa ccgatgctgc ccgcctgtaa
     7081 tgacctgtgg gtattgctga gcctgagccg cgtgcagctt gagcggctgc gccccctgct
     7141 ggatgcgcag tcggatgaat gtccggcacg tcagccacag gccaccgaac cgctgacatc
     7201 cgcgcaacca gagtcggcaa cgacggacgc accggcaaaa acgctgacgg ggaaacagga
     7261 tgacgccctg ctggcggtac ttaaccgctt taccgaagac gtgacggaaa aagcccgcag
     7321 cgggcgaatc gacccggtat tcgggcgcga caccgaaatt cgccagatgg tcgatatcct
     7381 ctcccgtcgc cgcaaaaaca acccgattct ggtgggagaa ccgggcgtgg gcaaaaccgc
     7441 gctggtggaa gggctggcgc tgcgtatcac cgaaggcaac gtgccggaca gcctgaaaac
     7501 ggtgcatatc cgcacactgg acctcggtct gttacaggct ggcgcgggcg ttaaaggtga
     7561 atttgagcag cgactgaaaa acgtgattga tgcagtacag aaatcaccgg agccggtact
     7621 gctgtttatt gatgaagccc ataccattat cggcgcgggt aatcaggcgg gcggcgcgga
     7681 tgcggcgaac ctgctgaaac cggcgctggc aaggggcgag ctgcgcacca tcgccgccac
     7741 aacgtggagc gaatacaaac agtattttga gcgcgacgcc gcgctggaac gccgcttcca
     7801 gatggtgaaa gtcgacgagc cggatgatga caccgccagc ctgatgctgc ggggactcaa
     7861 ggcccgctat gcacagcacc acggcgtaca tatgctggac agcgccattc agaccgccgt
     7921 gcgcctgtcg cgccgctatc tgaccggacg ccagttgccg gacaaggcgg ttgatttgct
     7981 ggataccgcc ggggcaagag tccgcatgag cctcgacacc ctgccggaac cgttgacgca
     8041 gcttcaggcg cgtctgaccg cgctggatat tgagcgggaa gcgattgagc aggacagcgt
     8101 gttttatccc gaaaccagcc cggagcggct ggcggaactg accgatttgc gtgatgagct
     8161 acaggcagaa gccgggcatc tggaagcgca gtatcagcag gaaaagcggc ttgcgcagca
     8221 gattatggcg ttgcgtcagg aaggggcaga cagcactgat ttgcaacagc aactgcgaac
     8281 gcatcagggc tttgcaccgc tgctggcgct ggatgtggac gcccgcgccg tcgccactgt
     8341 ggtggcggac tggaccggca tcccgctctc ttccctgctc agggacgagc agagcgacct
     8401 gctcagtatg gaacaaagcc ttgaaaaccg cgttgtcggg caaagcccgg cgctctgcgc
     8461 catcgcacag cggctgcggg cggctaaaac cggcctcacg ccggagaacg gcccgcaggg
     8521 ggtattcctg ctgaccggcc ccagcggcac cggtaaaacc gaaactgcgc tcacactggc
     8581 cgacaccctg tttggcggtg aaaaatccct tatcaccatc aacctctccg aatatcagga
     8641 gccgcatacc gtctcccagc ttaaggggtc tcctccgggc tacgttggct acggtcaggg
     8701 cggcgtactc accgaagccg ttcgcaaacg cccgtacagc gtggtgctgc tcgacgaagt
     8761 ggaaaaggcg catcgcgacg tgatgaacct gttctatcag gtatttgatc ggggctttat
     8821 gcgcgacggc gaaggccggg aaattgactt ccgcaacacc gtgattctga tgacggctaa
     8881 tctgggcagt gaccacatca tgcagctgct ggaggaaaaa ccggacgcca cggacgcaga
     8941 cctgcatgaa ctgctgtacc ccctgctgcg cgagcacttc cagcctgcac tgatggcgcg
     9001 ttttcagacg gtgatttacc gccctctggg acaggaagcg atgcgcgcca ttgtggaaat
     9061 gaaactggcg caggtggttc gtcgtcttcg tcagcactat gggctggaaa cggaaatcaa
     9121 cgacagcctg tatgacgcgc tgaccgccgc ctgcctgctg ccggacaccg gggcgcgtaa
     9181 tatcgacagc ctgctgaacc agcaaatcct gccggtctta agccagcagt tgctggcgca
     9241 acaggccgcg caccgtaaac cagcccagct acggcttggc tgggatgagg aagacgggat
     9301 tgtactggag ttcgctacgg aagagatgca ataacggagg ctgtcatggg tattcagaat
     9361 ttcgatcatt cacaccacaa gctcaaaatt cgtggcctgc aaagtccggt ggatgtgctg
     9421 accttcgaag gccgcgaaca actgagtacc ccgttccgct acgacattca gtttaccagc
     9481 agcgataaag ccatcacgcc ggagtcggtg ctgatgcagg acggcgcatt cagcctgacg
     9541 gcaccgccgg tacaggggat gccggtacag actgccctgc gcaccctgca cggcgtcatc
     9601 accggcttta aacacctgtc atcgtcacag gatgaagccc gttacgaagt gcgccttgaa
     9661 ccccgaatgg cgctgctggc ccgcagccgc cagaacgcca tttaccagaa ccagacggtg
     9721 ccgcaaatag tcgaaaaaat cctgcgcgag cggcaccaga tgcgcggtca ggacttcgtg
     9781 tttaacctca aaagcgaata cccgtcccgt gaacaggtga tgcagtacgg tgaggacgac
     9841 ctgacgttta tttcacgcct gctgtcggaa gtgggtatct ggttccgctt tgccaccgac
     9901 gcccggctga aaattgaggt tgtcgagttt tacgacgacc agagcggcta tgagcgcgga
     9961 ctgacgctgc cgcttcgcca tccttcaggg ctgtttgatg gtgaaacgga agccgtatgg
    10021 gggctgaaca ccgcatacag cgtggtggaa aaaagcgtca ccacgcggga ctacaactac
    10081 cgcaccgcca cagcagagat gatgacagag cagcatgacg ccaccggggg cgataacacc
    10141 acctatggcg aagcctacca ctacgctgac aacttcctgc aaaaaggcga caaggaggca
    10201 gcggaaagcg gcgcgtttta tgcccgtatc cgtcacgaac gctacctgaa tgaacaggcc
    10261 attctgcagg gccagagcac cagttcactg ctgatgccgg ggctggaaat cagggttcag
    10321 ggcgacgacg cgcctgcggt attccgcaag ggcgtgctga ttaccggcgt gacggccagc
    10381 gcggcccgtg accgcagcta tgaactgacg ttcacggcga taccctactc agaacgctac
    10441 ggctatcgcc cggcgcttat cccccgcccc gtaatggccg gaacactccc ggcgcgggtc
    10501 accagtacgg tgaagaacga catctacgcc catatcgata aggacgggcg ctaccgggtc
    10561 aatctcgact ttgaccgtga tacgtggaag ccgggctacg aaagcctgtg ggtacgccag
    10621 tcgcgcccgt atgcgggaga cacctacggc ctgcacctgc cgctgctggc gggaacggaa
    10681 gtcagcatcg cctttgagga aggcaacccg gaccgaccgt atatcgccgg ggtaaaacac
    10741 gactcggcac acaccgacca tgtgaccatt cagaactaca agcgcaacgt gctgcgcacc
    10801 ccggcgaaca acaaaatccg cctcgatgat gagcgcggca aagagcatat caaggtcagt
    10861 accgagtacg gcggcaagag ccagttgaat ctgggtcatc tggtggatgc cggaaaacaa
    10921 cagcgcgggg aaggctttga actgcgtacc gatatgtggg gcgcggtgcg ggcgaagaaa
    10981 gggattttta tcagcgccga tgcgcaggat aaggcgcagg ggcaggtgcg cgaaatggct
    11041 ccggcgatgg caatactgga tggcgcacag tcacagatgc agtcgctgtc cactgatgcg
    11101 cagacgacga atgccgatcc ggcagatctg tcatcgcaga tagcgctgtt gcagcaaagt
    11161 gtaaaagatc tgacgcaggc cgtgatcctg ctaagtgcac ccaagggcgt ggcgatcgcc
    11221 agcggcgagc acctgcaact ggcggcgtcg aagaatgtga tagcgaacgc aggcaacaac
    11281 gcagatatcg gcgtagtaaa aaatatgttt atcggtgtgg gtcaggcgct gagcgtgttt
    11341 gtgcgtaaag cgggcatcaa gctgtttgcc aataaagggg cggtctcggt acaggcacag
    11401 aacgatctga tggaactgtt ggcacagaaa tcgatagaga tcaccagtac ggaagatgag
    11461 atcaagatca ccgcgaagaa gaaaatcacc cttaacggcg gtggcagcta catccggctg
    11521 gatgcctgcg ggatagaagc gggaacgccg ggagaatata acgttaaggc cggatattac
    11581 gggcgtaaac caaaggcgaa gctgacgccg gagctgatgg catttccggt gattaagtct
    11641 gaggatttta atcagagctt tattttgctg gatgaaaata ctgggcaacc acttattaat
    11701 tggccctatg aactagaact ggaaagtggg ctaaaaatga gtggaattac tgatgaaaat
    11761 ggaaatacag aactaatatc ctctgataag gaggaggtcg ttaatattag tgtgtttgaa
    11821 cccgatgagt ttttagacga tgacattaat taagacatat agcgagaata taacatggca
    11881 gataaatccg aaaagaaaaa agaaaccaca aataaatcag caaaaaaaac actaacgaag
    11941 aagagcgaaa aattaactcc agcaaaagac accgcaccaa aacaagtgaa tgtagatcca
    12001 gctataagat tgaaacaaat tcgtcaactt gtccgtaata ataataaatc catcatggga
    12061 gaggattata ttatttgtca aatctataaa gaatctagat ttaagcaatt tgcaggtaag
    12121 aataaacata atgcgaaagg cttaatgcaa atgcaacgaa atgcagtgag gcaggttttt
    12181 aaatatcgac aacaaaaaat aaaaggaaga atgacgactg ataaagagac aaatgaagct
    12241 ttcgctaatg ctgatacatt ttataaatct gataaaatat ttgatgaaaa agaaaatatt
    12301 aagattggca cagagtattt gcaatactgg atagataaag aagcaacaat tgaggaggca
    12361 tatcgtggaa cagatgaagc atactattct gttataaaac cttgcgctga aaaactggct
    12421 aaagatcctg ataacataca aatactaatg gagggaatag gaagatagta tgcgcattat
    12481 gttaatatta ctttgttttt ttctttcccc tgtttttcag gcgataagat taaaatcgtg
    12541 aaaacctgtc aactgaaaaa aaaatgaaag tgtgcagttg ctttccctta gaaccattga
    12601 cggtgataca ttatatctaa aattcagaaa tgttatcacc gaggcctttt tagatactcc
    12661 aactaaaagt tatgattttt ttggagatgt ggttctttct cagtgcataa atggttctct
    12721 tatctttgcg ctggaatatg gttctccata tataaaaggt tgtctggtaa ctggttggga
    12781 aaatgggttt aaaggtaaga actctcctga aggcttttgt ttcgcagaga gaaatattcc
    12841 cgaagcggta tggtttggaa aaaacaatat tttaattatc atccgaaacc aaatggatgt
    12901 tgggacatgg aaagggaaat atattatcta tgataatgcg aaaaagtcag gagaaagagc
    12961 atactcatca gataatctac catcaaaaga tggttacatg atttttgcat taaataaata
    13021 aaatgactct atagataact ttaaatttca cttggttatt aacttctgtc cgataatgca
    13081 aaataaaggg atgtactgat gcctgtccga cttaatacca ttcccgacgc agaatcctat
    13141 cccgcaccgc cagcccgcat taaatggctg tcagccttat tactgatgct gtctgttggt
    13201 gtggcaataa cctcattatt cgccagcgat gaactggcaa aaaacggccc gcatttctgg
    13261 gggctggcct gcggtgtgcc tgcctttatc tggtcgctgg ttgcttcagt tcgctggttg
    13321 ttttttatca cgcagtacat tcgggctgat gcctggaata agcgccggga agaggtgatt
    13381 ttgcaggaaa cgcgacgggg acgacgggcg ttgcagatac ttagtttttc cgtacaaacc
    13441 gcgctgaatg gtgattccgt tgcagaaact acaacagcat ttctggcgcg tcagcaggtg
    13501 ttgaatactt acgctgattt gcgcggagaa gatactgtgc gtcgcagtgt cattcccgta
    13561 ttaaccaata aaccggttac cgggcgttta tccgaaatta tttcccgcct ttttattgat
    13621 atcagaccgc aattgtttct gctgccccct gattttcata tcaacgtact actggaaatt
    13681 gatgcgccgc tttcaggggc ctccgtgaga actatctggc aggacgaatg gcaaaaagcc
    13741 ggactcccgg aagcccgtct gttttctgcc ccggagccgg ggctggccgc cgtggacgac
    13801 tggctggaca actttattca ggaaaaggcc gtactgctgg tgatttccgt gtatctggaa
    13861 ccgaagactc cggaacggac ggcagaatcc tccaccgcgc tgttgctggc aaaccggttg
    13921 acgcaaaccg cgctgacgcc gctggccctg ctgcatcgtc ctgaacgtat cacagataca
    13981 gagatgatgg cgtcaggcat tgcacaggcg ctggactgga tgccggtgca gccagatgcc
    14041 atatccggca tatggactgc cgaactggac agggaacagc gtaccgcgtt gttatccctt
    14101 aaccagccgt ttactcagga agccctgatg tacgagctgg atgcctttct ggggcgctcc
    14161 ggaccggcag cgccgtggct gtccgtcgct gcggcaacgc tggcggcaat acaatcacaa
    14221 cacccccaac taacgctcag cggcgttcag ggcgggcatt attcatgggc gaccgtggtg
    14281 tcgccattcg tttccccaca ggaggcatca tagtggttcc gggattaaac cgtctccggc
    14341 cccgttctgt ttcagggcct gccatcgtca tgaccgtggc tgttgtggtt gcgctttgca
    14401 cgtttattca ccgtaaaccg gcattgctgg gcgttgaacc gggtagctca cagcaaatgt
    14461 actggatggc tggcgtgatt gccgcaggcg tggtcttttt gattatcatg ggggtttatc
    14521 atctgctgaa ccgccacgct gccgtacaaa actttgaact ccgcagacaa cttgccgccg
    14581 gagatgacta tcgtgcggtg acgacaaaga aagccgacag ccgtcatccg gcaatggtgg
    14641 aactcgccgc cttcctgcgc gaccgctacg gtccgttctg gcggcgtaac gtccgcctgc
    14701 tgctggtgac cggcgagcct gaacaggcag aagccatcgc gccggggctg accgggcaac
    14761 actggctgga gggcgaccac acggtgctga tatatggcgg caggccatca gcggagcctg
    14821 atgtcacact gctgaccgcc ttaaaaaaac tgcgccgcag ccgtccgctg gacggcatca
    14881 tctgggcgct gacagaagaa cagagccgcc agacagcgca actcgacaaa ggctggcgcg
    14941 aactgataaa cggcggtaag cgactcggtt ttcaggcccc actctatttg tggcaggtct
    15001 gtgacaacgg tgattatcag gccggacgcc ccctgcaaag cgtcggctgc ctgctgccgg
    15061 aacgctgcac cccggaacaa ctgaccgcca tgctggaagc tcacaccctg ccgctgacgg
    15121 aacagggcat gtcgcaactg ctggctgata accgccatga ttttctgctg cgtctggccc
    15181 atacccttgc agagcggggt atcgctcact ggcaaagcgt cctgaaaccg ctgctggcag
    15241 gcggcgcatt ctcttccctg cgcctgcgtg gtctgatgtt cagcccgccg cttaccgccg
    15301 taccggaggc cgcacctcat gcgtggctgc cgtcacaggt ctgggcgggc gtgaccggcg
    15361 ataacgcacg ggggcgtgca gtgggctttc cgtggctacg cgccgcgctg atgtccgctg
    15421 tctgcgtgct ggcaatatgg ggggccggaa tgacgacctc tttcttcgcc aactgcgctc
    15481 ttgttcagga aaccggtatc cagacggcgc gggcgctcga tacccgcctg ccgctggcag
    15541 aacaactggt ggcgctgcat accctgcagg gcgaactgga acgcctgcaa tatcgtatcc
    15601 gcgaaggtgc gccgtggtat cagcgttttg gccttgaacg taaccaacaa ctgctcgccg
    15661 ccgcctttcc cggctatgcg caggcggcaa accggctggt gcgcgacgtg gccgttgacc
    15721 atctgcaaca gcaactgaac gcctttgtcg ccctgccgcc caacagtcct cagcgtaccg
    15781 ccaccggtga acaacgctat aagcagctta aggcattgct gatgacttcc cgcccggaaa
    15841 aggccgacgc tgcctttttc agtaccacgc tgatggcaga cagcctgcgc tacgagaata
    15901 tcccggaagg tgtgcagcag agcgtgtttc cgtcactgct gaccttctgg atggcgaacc
    15961 tgccggaaca cccgcagtgg aaaacctcgc cgccaccgga actgaccggc gcggtgcgta
    16021 aaatcctgct gcgccagatt ggtgtgcgta atgccgaaaa cactctctac cagaacgtac
    16081 tgcaacaggt gtcccgcaac tacgccgata tgacgctggc ggacatgacc ggggataccc
    16141 tcaccgaatc tcttttcagt acggaacaga cggtgccggg gatgttcacc cgtcaggcgt
    16201 gggaaggaca ggtcagggaa gccatcgagc aggtggtgac ggcgcggcac gaggaaatcg
    16261 actgggtgct cagcgaccgc cagcaggata cctctgcgga tatatcgccg gatacgctgc
    16321 gtaaccgtct cacctcacgc tactttaccg acttcaccgg aagctggctg gcgtttctca
    16381 acagcattcg ctggaaaaag gaagactcgc tctccggcat tctcgaccag ctgacactga
    16441 tggccgatgc ccgtcagtcg ccgctgattg cgctgaccga taccctcgcc tggcaggcgg
    16501 cggcaggcag ggaaaaccgt ggcctgtcag actcgctggc gaaatcggca caggaactgt
    16561 ttaacggcaa ggagaaaacg ccgcagcaat cccgtgaagg cgacgtgcct gtcgggccgc
    16621 tggataaaac cttcgctccg ctgctgcggt tgctgggcga taaagccgga ggcggcgaca
    16681 gccagctgag tctgcagacc tacctcaccc gcgttacccg tgtgcgtctc aaactgcaac
    16741 aggtgaccaa cgcccccgac ccgcaggaaa tgacgcaaca gctggcacag acggtactgc
    16801 agggcaaaac cgttgacctc accgacaccc gcgactacgg acggttaatc gccgccagtc
    16861 tgggcgagga atggagtggc ttcggtcagg cgctgttcgt ccgcccggtg gagcagtcgt
    16921 ggcggcaggt gctgacgcct gcggcggaca gcctgaaccg ccagtggcag cgggcgattg
    16981 tcagccactg gaatcaggac ttcgctggtc gctacccgtt caaagcctca cagaacgatg
    17041 cctccctccc cctgctggcg cagtatctgc gcgatgacgg gcgcatcaac cagtttattg
    17101 ccgccaacct ttccggcgtt ctgaaacgtg aaggccgcta ctgggtggcg gacgccatga
    17161 acacgcaggg gctgacggtc aacccggact ttatccgcgc cctgaaccgc ctgcgcgacg
    17221 tggctgacac cgcctttgcc agcggcgatg ccggaataca ttttgaactg caggcaaaac
    17281 cggcccgaga cgtgatgaag acacatctgg tgattgacgg gcaggaactg gagtatttca
    17341 accagaaaga acgctggcag cgtttcaact ggccggatga acagtggcaa cccggcgcat
    17401 cgctgagctg gaccagtacg caggcgatgg aacgcatact ggcggattac cgggggagct
    17461 ggagccttat tcgcctgctg gaacaggcgc aggtgacacc ggtggacagc agcaccttta
    17521 aagtggtgtg gaaagcgcag gacggcctgc cgctgaatta cctgctgcgg gttgaacagg
    17581 gtaaagggcc gctggcgctg ctggaactga aaaacttccg cctgccggga caggtatttc
    17641 tgaccggcag gagtatgaaa gatgcggaag aatacgggga agacgccgat gagtgaactg
    17701 ttgcaaaagc tgacccgtag ctgttttgtc gaccgggatg cactggatgt ggcgcgaaca
    17761 caggcagcgc tgtggcagac atggctgctg ccggtgacgg caaatacgcc ggtcggggaa
    17821 gaccccggct accacgacga tttcctgcgt atccgcgacg agatgaacaa actttccggc
    17881 gcggatacgg acctcatctg ccagcttgcc gaatccctgc tgctgacgca ggcaaaagac
    17941 gtgcgcatcg ccacttacta catctgggcg cgactgcacc gcgacggtga gcgcggactg
    18001 gctgaggggc tggcgctact gaccggactg gtggaacgct tcggtacgca actgctgccc
    18061 tcgcgaccgg ccagccggaa aatggcgctg gagtggctgg caggggaaaa gatgctcgac
    18121 agtcttgccc gctacccgga agtggcaaaa gaggattttg cgaacattgt tgctgccctc
    18181 agtcagttga cggtttcctt cactgcatgg cccgaagacc agcattcccc ctccctgatg
    18241 ctgctgatta acgcgctgga atcacgtctg gcgcagtcgg gcgggatgaa cgcggtggtg
    18301 ccgcaaaaca gcagcagcgt tcctgcaccg tcatccccgg tggatgcgcc acaggtacag
    18361 accatcacct ccgggcgtga cctgctcgac caggcgaaag tgctggcccg ttatctgaac
    18421 gaacagccgc agggctggct ttcggcgcac cggctgatga aaaccctgcg ctgggacaca
    18481 gtgcatgaac tgccgccgga tgtggacggc aaaacccggc tggccccgcc gcgcaccgag
    18541 tcacgcaacc agcttaagcg gctgtatgcg cagcagaact ggacggaact gctggagcag
    18601 gccgacctga tgttcagcac cggcgtcagc catttctggc tggatatcca gtggtatctg
    18661 catcaggcgc tgactaaggc gggcgcaccg tgggacaggt ggacagccgt tatccgtcag
    18721 gacctggcgc tgctgctgga acgtctgcca gggctggaaa atctggcatg gaacgacgac
    18781 acaccgttcg ccgacgaggt gacgcgcaac tggatagcgc agcaggtcat gatgcgggaa
    18841 gacggcgcat ggcttgcagg gaaagccgct gtaccgacag acgacgccac aaatgacgta
    18901 ctggcgctgg agccggaagc actggagatg gcagacagtc agggcgtgga agcggctctg
    18961 ggctggatac agacccgtcc gggcatcacc acggcacgcc agcgcctgtt attgcgactg
    19021 ctgatggcgc gggtggcgga acagtacggt aaaaacgaaa tggcgctcct gctgctggaa
    19081 gaactggata ccgccgcaca ggggctgaca ctgacgcagt gggagccgga cctgctgttt
    19141 gaggtaaaag cccgacaact gaaactgctg cgcctgcggg cgcaccgcta tgcggataaa
    19201 gccctgctga accgaaaaat ggaaatcctg ctgggaacgc tggtgacaat cgatccggta
    19261 cgggcagcgg tattgtgtga tacgcaacat aaggactgat ggcaatggga acaggctact
    19321 ttctggtcag gggggacaaa accacctgcg gcgggaagat tattggaggc gcggacgacc
    19381 atacgatcat gggtatccca caggcacggg atatggaccg ggtcacctgc ggcagatatc
    19441 cgggaatgtt tattattgtg ggcggcgttc ctgaaacaga tattcacggc aggctgatgg
    19501 cggggacgct ggacagccag agcagttgcc cctgtaaggc acggtttatt gcgtcgatga
    19561 tggatgatac gtatgagacg gatgacgggg gaagtgaagc ggaacaacat gcgcagtcgg
    19621 cgaaaaagga tctgacttct ggttctgatt catcctctga tgatgtaaaa ctggattaca
    19681 gaattaaatt atcaggtaat aagatactga cgccactgaa tatacctgat tataaagaaa
    19741 tgataagcgg tggtagtacg aagaatacag aaaaaataga ttttactatc acaaataaag
    19801 gcgatgaggc tgaaggactt tctctggaag tgcttgatgg taatgaagtg atttacagtg
    19861 aacagcagac cggtaaatat tttgataagg gagagcatgc ctggcaatgg gatggctaca
    19921 gtaatcaagg catactggat acaactaagc taaaaagcaa atcactactc gtaagactga
    19981 ttgctttatg tggtgatatg atgataaaag ttgattatcc acttcataat tcaccagagg
    20041 aacaaaaatg ggtcgatgta aaggtagaca gaaaacaaaa aacagtagat ataatatggc
    20101 gactggcagt tagtgatgga gggataaaag gaagtaatcc taagttatcc ccagttcctt
    20161 ataacgactt ggttaacctg accaaaaatg gagttgaatt ttattggtct cgaaatggaa
    20221 gtcgcggcgg ggggattggt gaaaatattg ttacagcgat tggtgttttt aaagtaaatg
    20281 taaaggctga gataaatatt actccatcca tgcgaacttt ttctttgata tcatcattag
    20341 atcctgactt tcaggcatcc gttagtctat caggatttga aaaaatatat tataactacg
    20401 gtgatagtta taaggatatt caagatgaat tacaggcgct tcttgacgct aataacagat
    20461 ataaatggga tagtgctcat gaaatggggc ataaagtact cgatgaatat ggagagggtt
    20521 ctagccctga ttattcgtgg actcataaag gaacatctac tcttatgcaa aaaacaatac
    20581 cagggaatgt tatgcccgct cagggagaga ttgatgtaat gaaatatgga aaatatcgtc
    20641 ctgacatgta tactcgattg gttgcagcag atgaagatgt tcagggattg atctggttaa
    20701 gcaggataaa atttgatgac tagagtagtt attgctttat tatttcttgc cggatgtact
    20761 tcgcaaatga agtattccaa tgtcgtcagg attaggaatg tatgttcctc agatattaat
    20821 gtgtttattc ctgagtatgt ttttggaggt aatgttatta agcaacatca tatttactta
    20881 cctgttaatg ggattgatgt tttgtcttat ttttatactg aaaacatcaa tgatggtttc
    20941 caatatgaag aagcattcac cgtaaataaa gatagctcgg gtaattttga ttatccgttt
    21001 gttatcttat catcaggacg tgaaaaaaaa tttgggaaaa acgatctaat tgaattatcc
    21061 agggacattt caacaaaaga cgataagaag catggggttg taaagtacat gatagatagt
//
LOCUS       DAZFYC010000080        20900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFYC010000080 DAZFYC010000000
VERSION     DAZFYC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.080"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..126
                     /locus_tag="ACHKW5_003335"
     CDS             <1..126
                     /locus_tag="ACHKW5_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFO7512674.1"
                     /translation="IDFRNIYSEYYSATKNDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            165..382
                     /locus_tag="ACHKW5_003336"
                     /pseudo
     CDS             165..382
                     /locus_tag="ACHKW5_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            492..755
                     /locus_tag="ACHKW5_003337"
                     /pseudo
     CDS             492..755
                     /locus_tag="ACHKW5_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            978..1103
                     /locus_tag="ACHKW5_003338"
                     /pseudo
     CDS             978..1103
                     /locus_tag="ACHKW5_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1163..1669)
                     /gene="yciE"
                     /locus_tag="ACHKW5_003339"
     CDS             complement(1163..1669)
                     /gene="yciE"
                     /locus_tag="ACHKW5_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7512675.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKIAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1715..2215)
                     /gene="yciF"
                     /locus_tag="ACHKW5_003340"
     CDS             complement(1715..2215)
                     /gene="yciF"
                     /locus_tag="ACHKW5_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7512676.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2301..2480)
                     /gene="yciG"
                     /locus_tag="ACHKW5_003341"
     CDS             complement(2301..2480)
                     /gene="yciG"
                     /locus_tag="ACHKW5_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7512677.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(2861..3667)
                     /gene="trpA"
                     /locus_tag="ACHKW5_003342"
     CDS             complement(2861..3667)
                     /gene="trpA"
                     /locus_tag="ACHKW5_003342"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO7512678.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(3667..4860)
                     /gene="trpB"
                     /locus_tag="ACHKW5_003343"
     CDS             complement(3667..4860)
                     /gene="trpB"
                     /locus_tag="ACHKW5_003343"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO7512679.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     AREEI"
     gene            complement(4872..6233)
                     /gene="trpCF"
                     /locus_tag="ACHKW5_003344"
     CDS             complement(4872..6233)
                     /gene="trpCF"
                     /locus_tag="ACHKW5_003344"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO7512680.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(6234..7829)
                     /gene="trpD"
                     /locus_tag="ACHKW5_003345"
     CDS             complement(6234..7829)
                     /gene="trpD"
                     /locus_tag="ACHKW5_003345"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO7512681.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(7829..9391)
                     /gene="trpE"
                     /locus_tag="ACHKW5_003346"
     CDS             complement(7829..9391)
                     /gene="trpE"
                     /locus_tag="ACHKW5_003346"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO7512682.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9413..9506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9483..9527)
                     /gene="trpL"
                     /locus_tag="ACHKW5_003347"
     CDS             complement(9483..9527)
                     /gene="trpL"
                     /locus_tag="ACHKW5_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO7512683.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            9665..10546
                     /gene="rnm"
                     /locus_tag="ACHKW5_003348"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             9665..10546
                     /gene="rnm"
                     /locus_tag="ACHKW5_003348"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO7512684.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10543..11163
                     /gene="yciO"
                     /locus_tag="ACHKW5_003349"
     CDS             10543..11163
                     /gene="yciO"
                     /locus_tag="ACHKW5_003349"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO7512685.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            11264..12136
                     /gene="rluB"
                     /locus_tag="ACHKW5_003350"
     CDS             11264..12136
                     /gene="rluB"
                     /locus_tag="ACHKW5_003350"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO7512686.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(12176..12766)
                     /gene="cobO"
                     /locus_tag="ACHKW5_003351"
     CDS             complement(12176..12766)
                     /gene="cobO"
                     /locus_tag="ACHKW5_003351"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO7512687.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(12763..13521)
                     /gene="yciK"
                     /locus_tag="ACHKW5_003352"
     CDS             complement(12763..13521)
                     /gene="yciK"
                     /locus_tag="ACHKW5_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO7512688.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13741..14790
                     /gene="sohB"
                     /locus_tag="ACHKW5_003353"
     CDS             13741..14790
                     /gene="sohB"
                     /locus_tag="ACHKW5_003353"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO7512689.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGEYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14826..15077)
                     /gene="yciN"
                     /locus_tag="ACHKW5_003354"
     CDS             complement(14826..15077)
                     /gene="yciN"
                     /locus_tag="ACHKW5_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO7512690.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15457..18054
                     /gene="topA"
                     /locus_tag="ACHKW5_003355"
     CDS             15457..18054
                     /gene="topA"
                     /locus_tag="ACHKW5_003355"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO7512691.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKAGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGGALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            18264..19238
                     /gene="cysB"
                     /locus_tag="ACHKW5_003356"
     CDS             18264..19238
                     /gene="cysB"
                     /locus_tag="ACHKW5_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO7512692.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            19557..19697
                     /gene="ymiA"
                     /locus_tag="ACHKW5_003357"
     CDS             19557..19697
                     /gene="ymiA"
                     /locus_tag="ACHKW5_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO7512693.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            19700..19867
                     /gene="yciX"
                     /locus_tag="ACHKW5_003358"
     CDS             19700..19867
                     /gene="yciX"
                     /locus_tag="ACHKW5_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO7512694.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            19981..20076
                     /gene="ymiC"
                     /locus_tag="ACHKW5_003359"
     CDS             19981..20076
                     /gene="ymiC"
                     /locus_tag="ACHKW5_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO7512695.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            20240..>20900
                     /locus_tag="ACHKW5_003360"
     CDS             20240..>20900
                     /locus_tag="ACHKW5_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO7512696.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
BASE COUNT         5194 a         5115 c         5361 g         5230 t
ORIGIN      
        1 atagattttc gaaatattta ttctgaatat tactctgcaa ccaaaaatga taaagagatt
       61 atcgatcgac gattacagat gcgtacaaat gatttttatt cattattaaa cagaaaatat
      121 ttataagtgc tatattcatg aatgaaaaat agctatatgc gtacattctc aacctgcccg
      181 tcacgtagca ggttgaatca ctttttcttg ataaagggct ggtcctgcca ccgttgttgt
      241 tgggtgcgtt aaaaatacac tactaatatg cccggcatgc gggcaacaga gtcctgttca
      301 cgatcgccgc caccggaaat ggcgctgcct cgatacctgc cagttcatgg cactattcga
      361 agccgatgtt ccccacgtta tatggagtaa tcattggggt tatgccgcag cacgttgtat
      421 gcaagaatgt gctgcggctg gtttgtgagc tttcgatagt ggttgttatt tttgccctta
      481 tttgttccgg aggccctggt tcaatggtcc gtctaccccc tgtggtgatg tcagcaaaat
      541 cagccactgc gcgaaccaca atagcccggg aagatgctga agatcaccag gtaaagctgt
      601 cagcgcagaa actggaagaa ctgctcgcat caatggttaa ggatgaggtt gatcgcaatg
      661 atgggattta ttgacgtcag cgagagcaga aggaagaact gaataacctg aatgatttac
      721 gctcaatcag agcgatgatg attggcagta attagcggtc aatttcgggt tttttggctg
      781 tgcgctcccc gcccagcatt gcgcgtcaat tagccccaaa aaaacgaaaa agttacaatc
      841 aagtaaaaaa ttccttctgc aaaactaaaa aagatttcaa taaaatcagg cagttaaaaa
      901 cataaaaaaa aggtacaaaa aagcatctca aagtcttgtt ttttgcttca aatacaattg
      961 gttattgtgt tttttgaaac ctaccagtgt ggtacatgga tatcgatacc accgccaatt
     1021 ataagctagg cggtgcacag caacacgata gcgtacgcct cgatccgtgg gtgtttatgt
     1081 tctcagcagg atatcgtttt taattccaca aaaaaacgac cccgtaatat acggggtcaa
     1141 taaggacatg gcataaagtg tattatttct tcgcttctac gccgtcagtt tcagagcgaa
     1201 ttaaaaattt ctcagttgtt tgcggaatat gctgaatcag ccagtcggcc atttgctttt
     1261 cctcatttaa aatcgcttcg atggttggaa ttgaagctgt atcaccggca ttttttgctg
     1321 ctgctaatag tgaggtgtaa caggcgattt caaattgctc gaagacatat ccgctaatag
     1381 agcctttgac tatttcatca gaagggaata taccaccgat tgactgccca agcgcagcta
     1441 ttttactcat ggaatcttta atgactgaac gtgaaatgtc attacgatca agaatagttt
     1501 ccagttgaac aatctggttt ttggtttcac taagatgttg ttcaatacga gcgcgtagtt
     1561 caggataatt atctatacgg ctggccatgg attcaagcat agattcggct tgcttttcca
     1621 ttgcgtgggc gtcacgtaac cagtcatgat aatgttcaat acgattcatg aaaatgtcct
     1681 ctttataaaa tgactaaaag ttaaagttca tatttcaggc tttattttcg gctttcttat
     1741 ttacgttatt aagggccaga tcagtcagtt tgatgtcggt ggccttttct tcttccaggg
     1801 tctctttcag aagcttcgct gctttacgat aacctaattg ttcagccagc gtcgctaatg
     1861 tcccgtaact ggcaatctca taatgctcga ctttctgtgc tgcggcaatc agtgcggcat
     1921 cacgcacttc gtttttctcg gtactttcga tgacctcatt agcttcttca ataagacctt
     1981 ccattgccac acatttcatg cgcttaattt tcagattcga ttctgattcc acaacttgat
     2041 caatacgttc aatctgtcca tgtgtttcct cgaggtgcgc atgaaaagcc tgacttaatt
     2101 tttcgtttga tgttgctctt gcgagttttg ccagtgcccg ggttaattgt ttttctgcgc
     2161 tgtaggtatc tgaaagcagg tgaataaata catcttcaat ggtcttcata ttcatatttt
     2221 tctccagtga aatcactgcg agccatacgg cccgcagatg caattatctt tatcagctga
     2281 tattaataat taacggtgaa tcaggatttg cctgatttat taccaccgct ttgttgaccg
     2341 ccttttttac ccgcttcaga tgcgcgttgc ggatcatttt taaaattacc gccgctatgc
     2401 tgaccgcctt tacggcctgc gtcggatgcc ttctcacggt cttcggcgaa atttcctgaa
     2461 ccaccacgat gttcggccat gttatttctc ccgttgcgtt gcattgtttc attaatatga
     2521 gtgttgtgtg tcgacactca ttaaaattag tcgctaatga gaattagtca aattaagcgc
     2581 aacgagaaga tagagggaaa atatattttg aggaacattc tggatatatt aacaattacc
     2641 tgaggaataa gtgacttaga gaaaattatt gattttactg gtgttatgtt gcgggattaa
     2701 tttgtttgat tatattactg ttgggcggaa aaatgacgta agttgacgtt cgaccggggt
     2761 aagcgaaacg gtaaaaagat aaatattaaa tgaatttagg atttttccgg cttcattaaa
     2821 gaaagttaaa atgccgccag cggaactggc ggctgtggga ttaactgcgc gtcgccgctt
     2881 tcatcggttg tacaaaaact ttcagtgccg ccagcatttt ctctggctca ttaatatgtt
     2941 gctcgatgat tttaacaatg gccgaaccag aaatcgcgcc cgcagctcct gcatcaatcg
     3001 ctgcttttac ctgatccggg gcggaaatac caaacccctg caatggaggt gcagcgttgt
     3061 actctttcag cttcgcaacc agatgattga ggggtaacgc ggcgcggttt tctgcgccgg
     3121 tcacgcccgc tcgtgacagc aaataggtgt aaccacgacc gtaagaggct atctggcgca
     3181 gcaggtcgtc atcggcattt ggcgggcaga tgaagatagg tgcgacatta tggcgcaacg
     3241 cggcctggcg gaagggcgcg gactcttcaa ttggcacatc ggcaaccagc accgaatcga
     3301 cgccgacttt ctcgcactgg gcataaaact catcaatgcc tttgttaaac accagattgg
     3361 catacatcaa caggccaatg ggaatggtcg ggtgtttctg gcgaatcagt gccagcattt
     3421 caaaacattg tgccggagtc acacctgccg caaaggcgcg cagagtggcg ttttgaatgg
     3481 tcgggccatc cgctagtggg tcggagaagg ggatacctaa ctccagcgcg tcagcaccgg
     3541 cttcaattag cgtatcgata attttcaatg actgctcaat gcccggatca ccgagcgtga
     3601 cgaaaggaac gaatgcgcct tctttgcgct ccttcaactg ggcaaacaga gattcgtagc
     3661 gttccatcag atttcctctc gtgctttcaa aatatcgtga acggtgaaga tgtctttatc
     3721 gccgcgaccg gaaaggttaa ccaccagtag ctgctcttta tccgggtttt cgcgcatcat
     3781 tttcaacgca tgggccaggg cgtgggagga ttccagcgcc gggatgatcc cttcgtgcag
     3841 gcacagcgtt ttgaaggctt caagggcttc atcatcggta atagacacgt aatcagcgcg
     3901 tccagtgctg ttaagatacg cgtgttgtgg gccgacagac gggaaatcca gtccggcgga
     3961 gatggagtaa gattcttcaa tctgcccgtc ttcggtttgc atcatcggcg ctttcatacc
     4021 gaaatagatg cccacgcgac catgttttaa cggtgcgccg tgctcgccag tttcgatacc
     4081 gtgaccacct ggctccacac caatcaggcc gacgttggtt tcattgatga aatcagcaaa
     4141 catgccgatg gcattcgaac cgccgccaac acaggcgata acggcatccg gcaggcgacc
     4201 ttctctttcc agaatctgcg ctttggtttc ttcgccaatc atccgctgaa actcacgcac
     4261 aatggtcgga taaggatgcg ggccagctgc ggtgcccagc atatagtgcg cggtttcgta
     4321 actaccggac cagtcgcgca gcgcctcgtt acaggcatct ttcagcgtcg cggaaccgct
     4381 atgcaccggg atcacttccg cacccattaa gcgcatacga aaaacgttag gcgactggcg
     4441 ttctacgtct ttggcaccca tataaatacg gcatttcagg ccgagcaggg cgctggcaag
     4501 ggccgacgcc acgccatgct gaccggcacc ggtttcggcg atgatttcgg ttttacccat
     4561 ccgcttcgcc agtaaagcct gaccgagcac ctggttagtt ttatgcgcgc cgccgtgcag
     4621 caaatcttca cgcttgagat acagagtggt gttcgtcccg gctgtaatgt tctggcattt
     4681 ggtcagcgcg gttggacgcc cggcatagtt tttcagcagg tcgttgaact gagcctgaaa
     4741 ttcaggatct ttttgcgcac tgacaaaagc ttcttccagc tggcgcagag caggcatcag
     4801 gatttgtggc acgtacatgc cgccaaactc accaaaatag gggttaagta atgttgtcat
     4861 tgttcctttc cttaatatgc gcgcagcgtc tggaaaaccg aggccaaaag acgtgcgtct
     4921 ttgatgcccg gttgcgactc tacagcagaa ttaaaatcaa gtccggcgca gccggtttgt
     4981 gccgcttcca cgcagttatc tgcgcctaag ccccccgcca gcagaacgtt gccaagcgat
     5041 tgaccattta atagtgacca gtcgaaacgt tgcccgcttc caccctggcc gttgtctaaa
     5101 acatatttat caacgtgctg aagctcgcgg gcgggcaggg tttcaccgac gcttaatgct
     5161 ttccagatgg caacgtgtgc tggcagagct tcacgcagcg tatcgatata cagctgatct
     5221 tcattaccat gcagttgcac tgccgccagc gataacacct tagctttgtc caccacatcg
     5281 gcaatatcgt gattgcggaa cacgccaaca tactgcaacg gtgccgcagc catcacttcc
     5341 tgcgcctgtt caacgttgac gcaacgcggt gatgtcgcaa caaaaatcaa cccaccgtaa
     5401 atcgcgcccg cgtcataagc tgctttagca tcttgcccac gcgtcaggcc acatacttta
     5461 ttctcaccca gcaacacccg gcgcacggcg gcgtgcaaat cgtcatgggc catcaacgcc
     5521 gaaccaatca gaaaaccgtt agcgaagtgg cttaactcgc gcacctgagc gtaagtattg
     5581 atgccggatt cgctgattac cgttacgttg tgccccagtt tcggcgcaag ctcgcgggta
     5641 cggttgagat caatcgacaa atcacgcaga tcgcggttgt tgatgccaac gacctttgcc
     5701 cccaatgcaa tggcacgctc cagttcctct tcattactga cttcggtcag cacacccatc
     5761 tccagactgt gggcgacggc ggcaagctgg cgatattgtt cgtcatccag tactgaaagc
     5821 attaataagc aggcatcagc ctggtaatag cgcgccagat agatctggta aggatcgata
     5881 atgaagtctt tacataaaat cggctgcggg gcgatttggc tgacgatggg gaggaaatca
     5941 aagctcccct gaaaatattt ctcatcagtc agcaccgaaa ttgccgaagc gtaatgttta
     6001 taaatggcgg caatgcgtgc tggatcgaaa tcatcacgga tcacgccttt tgacggcgac
     6061 gctttcttgc actccagaat aaacgccgtg cgcgcaccct gtagcgcatc ataaaaatgt
     6121 cgcgtgctcg gctgaacctc attctgaaaa ctggccagcg gttgctgctg tttgcgggtt
     6181 tctacccaaa tcgccttgtc tgcgacgatt ttcgctaaaa cggtttgcat catttaccct
     6241 cgtgccgcca gtgcggtaac tctgtcgtaa gcggaaccac tgcgcagtac ctcaagaacg
     6301 gtttgcgcat tggcttgcag atcttcatgg ccatgcaggc gcattaacat ggcgacgttc
     6361 gcagcgacgg ctgcttcatg ggcggcgtcg cctttacctt gtaacaagcg tgttaaaatg
     6421 tcacggtttt cttccggtgt tccgcctgcc agttgctcct ggtggtaggg agtcaggcca
     6481 aaatcttcag cggtcaattg atagctctta atttcgccgt catgcagctc ggcaacgatt
     6541 gtcggcgcgt gtaatgaaac ttcatccatc ccgccgctgt gcaccaccgc cgcgcgttga
     6601 taccccagca cgcgcaaggt ttcggcaatc ggcagcacca gttccggact ataaacacca
     6661 attaacgcca gcggcggatg cgccgggtta atcaatggcc ccagcacatt gaacagggtg
     6721 cgggttttca gttgctggcg aaccggcatc gcgtggcgga atccggtgtg atacttcggc
     6781 gcaaagagga aacatacacc taactcatcc agcgcctggc gcgatttatc ggcgttcata
     6841 tcaagattaa taccgaacgc cgccagcaga tcggacgaac cagatttact ggagacgcta
     6901 cggttgccgt gtttcgccac tttcagccca caggccgcgg cgacaaacgc actggcggta
     6961 gaaatattga tactgttgct gccgtcaccg ccagtaccga cgatatcagc aaacagataa
     7021 tccgggcgcg ggaacggcgc ggcgttttcc agtagcgcgg ttgctgctcc ggcgatctcg
     7081 ttcgggtgct caccgcgaat tttcatgctc accagcgccg ccgccagttg ttccggcttc
     7141 agctcgccac gcaccaccgc tgaaaacagc tggtggcttt cttgttggct aagcgtctgc
     7201 gcctgataca gtttttccag aatcggttgc agcgtgttgg ctggctctag tttctgctgc
     7261 gcccaggcca gcgtttgttc cagcaggcga gcgccctggg tggtgagaat ggattccgga
     7321 tggaactgga atccacaaac gcgatccgca tcgtgacgta ctgccatcac catgccatta
     7381 aaatgggcgt tgatggttaa accggccgga atgttactgc caaccagcga gtgataacgc
     7441 gccaccggca gcgggtttgt taatccggca aacatcgcct gaccgtcatg ttcaatgctc
     7501 gacgctttac cgtgaagaat ttcgcccgcc tgaccgacat agcccccgta agcttcgaca
     7561 attgcctgat gtccgaggca aatgccaata atgggcagct tgccacgcaa gcgggtgagg
     7621 agttccggca tacaaccggc ttcgctcggc acaccggggc caggagaaag catcagtacc
     7681 ggattgctca tcgtcgccag gcgttcaatt aaggtctgcg ccggaatatg gttgcggtaa
     7741 atcaccacat tatgcccatt gctgcgcaac tgatctgcca ggttgtacgt aaaagagtcg
     7801 atattatcga gcagcagaat gtcagccatc agaaagtctc ctgtgcatga tgcgcggtgg
     7861 caatagcgcg cagtacagcg cgggctttat tacgggtttc gtcggcttcc gactgcggaa
     7921 cagaatcaag gactacgcca gcaccggctt gcacggtggc gataccgttt tccaccagcg
     7981 ccgagcggat cacaatgcag gtgtcgagat cgccatgcgc ggtgaaataa cctaccgcgc
     8041 cgccgtagct gccgcggcgg cgaccttccg cctcggcaat taactgcata gcgcgtactt
     8101 tcggcgcacc acttaacgtt cccatattca tacaggcgcg gtaagcgtgc agggcgtcga
     8161 gatcgtggcg cagctcaccg accacgcggg agaccaagtg catcacgtaa gagtaacggt
     8221 caactttggt gaggtcggcg acgtagcggc tgccgggggt gcaaatgcgt gccagatcat
     8281 tacgggcgag atccaccagc atcagatgtt cagaaagctc tttatgatcg gtacgcattt
     8341 ccagttcgat gcggctgtcg aggtctctgt ccagcgagcc atcggcacga cgaccgcgtg
     8401 gacgtgttcc ggcaatcggg tagatctcaa tctggcggct ggtggcgtca tacttgagcg
     8461 aactttccgg cgacgcgcca aacagggtga aatcattatc ctgcataaaa aacatgtacg
     8521 ggctgggatt actctttttc agcacgtaat aggccgccag cggtgacggg cagggcagag
     8581 agaaacggcg agatggcacc acctggaaaa tttctccagc gcgaatcgct ttttgcaaca
     8641 aacgcactac gccaccgaac tcttcatcgc tctgattaca ttcacaacgc atatgcggca
     8701 cggaaaccac tggcagcggc ggcgcggctt cggtcagttg ctgacgtagt tcgttcaggc
     8761 gagcagtgag acgttgtttt tcttcttcat tcggagcaaa caggctggcc tgaatacggg
     8821 tgcttttttt ctgatggtca atcaccatca gagtttcagc gagataaaaa cagaaatcag
     8881 ggcagttatt ttccgctgac agttgcggta aatcttcaaa tcccgccaca aggtcataag
     8941 agaacaggcc gccgaagaac attgcttctc gttcttcctt cggtacattc aacagattct
     9001 gcaataaacg gaaagcgtca aaaaccgaaa gggagcataa gcgagcgtct tcatccagca
     9061 gtggactgac aggggggaag cgcagcacac ggcagtttgg taattgttca ttttccacac
     9121 ccgcaggcag ggcgttatcc agtagtgtca gcagggcttc gccgttgccg gaaagtgcct
     9181 ggattgtgac agtgtcacct aaagctgtaa tgcgcagcgc actgtctacc agcagcaggc
     9241 tttttaaatc atctttgctg tcgatatctg cggattccag cagcagcgtt gccggacgat
     9301 ccccacacaa ctggtgaaaa agcgcggtgg gattgtcgcg ataagcgcct tcgcaggtta
     9361 gcagttcgag agtcggtttt tgtgtttgca ttgttattct ctaattttgt tcaaaaaaaa
     9421 gcccgctcat taggcgggct gggtatctga ttgctttacg catggtgaat acactgcccg
     9481 tttcaggaag tgcgccacca acctttcagt acgaaaattg ctttcattgt cgataccctt
     9541 tttacgtgaa cttgcgtact agttaactag ttcgatgatt aattgtcaac agctcatttc
     9601 agaatatttg ccagaaccgt tatgatgtcg gcgcaaaaaa cattatccag aacgggagtg
     9661 cgccttgagc gacacgaatt atgcagtgat ttacgacctg cacagccata ccacagcttc
     9721 cgatggctgc ctgacgccag aagcattggt gcaccgtgca gtcgagatgc gcgtcggcac
     9781 cctggcgatc accgaccatg acaccacagc tgcaatcgcg ccagcaagag aggaaatttc
     9841 acgttcggga ctggcgttga atcttattcc cggcgtggaa atttccacgg tctgggaaaa
     9901 tcatgaaatt catattgtcg ggctgaatat tgatattact catccgctaa tgtgtgagtt
     9961 cctcgcgcag cagacagaac ggcgcaatca gcgggcgcag ctgattgccg aaagacttga
    10021 aaaagcgcaa atacctgggg cgctggaagg cgcacaacga ctggcgcagg gcggcgcagt
    10081 gacgcgcggt cattttgcgc gtttccttgt tgagtgcggc aaagccagtt caatggcgga
    10141 tgtctttaaa aagtatctgg cgcgcgggaa aaccggatac gttccgccac agtggtgtac
    10201 aatagaacaa gctattgatg tcattcatca ttctggcggt aaggcggtgc tagctcatcc
    10261 tgggcggtac aatcttagtg ctaaatggct gaaaagattg gttgcgcatt ttgccgaaca
    10321 ccacggtgac gcgatggaag tcgcgcagtg ccagcaatcg cccaatgaac gtacccagct
    10381 ggcggccctt gcgcgtcagc atcatttatg ggcatcacaa ggatctgatt ttcatcagcc
    10441 atgcccgtgg atcgaactgg gtcgtaaact ctggctgccc gcaggcgttg aaggcgtctg
    10501 gcagctatgg gaacagccgc agaacaccac agagagggaa ttatgagcca gtttttttat
    10561 attcatcctg ataacccaca gcaacgtctg atcaaccagg cggtggagat cgtgcgtaaa
    10621 ggcggggtga ttgtttatcc aactgattcc ggctatgcgc tcggctgtaa aattgaagat
    10681 aaaaacgcga tggagcgtat ttgtcgtatt cgccagctgc cggacggtca caactttacc
    10741 ctgatgtgtc gcgatctttc tgaactctcg acctattcat ttgttgataa cgttgcgttt
    10801 cgtttaatga aaaacaacac gccgggcaac tataccttca tcttgaaggg gacgaaggaa
    10861 gtgccacgcc gtctgttgca ggaaaaacgc aaaaccatcg gtatgcgtgt gccttctaac
    10921 ccgattgcgc aggcgttgct tgaagcactg ggcgaaccga tgctttccac ttcgctaatg
    10981 ctgccaggca gcgaatttac cgaatccgat cccgaagaaa ttaaagaccg tctggaaaaa
    11041 caggtggatt tgattatcca cggcggctat ctcggacaga aaccgacaac ggttattgat
    11101 cttaccgatg atacgccggt cgtggtgcgt gaaggcgtag gtgatgtgaa gcctttctta
    11161 taagtgcgta actctgtata ctacgcggcc ttaaaatggc gctatacggc gccattgatt
    11221 ctcctgttcg acgcctgtga aggcgacacc aaaaggaagc tctatgagcg aaaaattaca
    11281 gaaagtgctg gcccgcgctg gccacggctc ccgccgtgaa atcgaatcta ttattgaagc
    11341 cggtcgtgtg agtgttgatg gcaaaattgc taaactcggc gatcgcgttg aagttacccc
    11401 tgggctgaaa attcgtatcg atggtcatct gatttcggta cgtgagtccg ctgaacaaat
    11461 ttgtcgcgtt ctggcctatt acaaaccgga aggtgagttg tgtacccgta acgatccgga
    11521 agggcgtccg acggtgtttg accgtctgcc aaagctgcgt ggcgcacgct ggattgccgt
    11581 aggtcgtctg gacgttaaca cctgtggtct gctgctgttc accaccgatg gtgaactggc
    11641 aaaccgttta atgcatccaa gccgtgaagt tgaacgtgaa tacgccgtgc gtgtatttgg
    11701 tcaggttgac gacgcgaaac tgcgtgattt gagtcgtggc gtgcaactgg aagatggtcc
    11761 ggcagctttt aaaaccatca agttcagcgg cggcgaaggg atcaaccagt ggtacaacgt
    11821 gactctgacc gaaggccgta accgtgaagt tcgtcgtctg tgggaagcgg ttggtgtgca
    11881 ggtgagccgc ctgatccgtg ttcgttacgg tgatatccca ctgccaaaag gtctgccacg
    11941 cggtggctgg acggagctgg acctcgccca gactaactat ctgcgcgaac tggtggagct
    12001 tccgccagaa accagctcta aagtcgctgt agaaaaagac cgtcgtcgca tgaaggctaa
    12061 tcagattcgt cgtgcggtga aacgtcacag tcaggtgagc ggcggtcgtc gttctggcgg
    12121 gcgtaataac ggttaatcaa agtatgccgg acgtcatatc cggcattttt acagattaat
    12181 aatcaatccc tatttgcgcc ttaacaccgg catcaaacgc atgtttgatg gggcgcagct
    12241 cacttaccgt atccgccagt tcaagaatat cccgatgaca gcctcgaccc gtgataatca
    12301 ccgtctgttg atgtggacgt tcatttaacg cctgcaccac ttcttccagt ggcaaatagt
    12361 catacgccac catatacgtc agttcatcaa gcaaaaccat atccagtgag gaatcagcaa
    12421 gcatccgctt tgcatgttgc cagacttcgc ggcaggcggc ggtatcagac tcgcggtttt
    12481 gtgtatccca ggtaaagccc gttgccatca cctgaaactc aacgccatgt ggctccagca
    12541 gattgcgttc gccattaggc caggtgcctt taataaactg cacgacgcct actttttttc
    12601 cgtgaccaac tgcgcgtgtt gccgtaccaa atgccgcggt ggttttgcct tttccattgc
    12661 cggtaaagac gatgataata ccgcgttcat cctgggcctg ggccacacga gcatctactt
    12721 tttctttcac tcgctgctga cgctgttggt agcgttcatc actcattggg aaattcctgg
    12781 tttacggccc ggttgggcgt caaaggtcat gccggtttta cggcggctgt catcgcccat
    12841 cagccagagg tagagcggca tgatatcagc gggtgtttta agtttctgtg gatcttcggt
    12901 cgggaaggcg ctggcacgca ttgcggtgcg cgtaccgcct gggttaatgc agttgacgcg
    12961 caggcgctgc tgatattcat cggccagtac ctgcatcatc ccttcggtgg caaatttcga
    13021 cgctgcatat gcgccccagt tggctcgtcc ctgacgtcca acgcttgatg aagtaaagac
    13081 cagtgaaccg gcgtccgatt tgagtaataa aggaagcagt gcctgggtga gcataaaggt
    13141 ggcattaacg ttgacctgta tgacgtcctg ccagacctgt ggattttgtt cgctcattgg
    13201 gcaaacatcg ccgagcaatc cggcattatg caaaacacca tccagacgcg gataattaac
    13261 ggtaatgcgc tgtgccagtt gttggcaatc ttcggacgtg caggtcagca aatcgagaat
    13321 aaaccactgt ggctgacgtc cagtttcttc gtttatgtgg ctggctacct gacgtaattt
    13381 ttcttcatta cggcccaaca gaatcactgt cgcaccatag cgtgcatacg tcatcgcggc
    13441 ttcacggcca atcccatcgc tggctcccgt caccaggata atgcgatcat tgagtaaatc
    13501 ttgttttggc tggtaatgca tggctactcc tcaacgacgt tgtctgtcgt gctgccagtg
    13561 tactttatga ctttggggct ttatgcctga aacagaaggg catttcaatc agcctggggc
    13621 gataacaccg caaaatggat actttgtcat actttcgctg caataacatc tctgcgagac
    13681 ggcttaacat gcctgttgta aactgtgagc caaagcgttg tttaaccaag gtggggactc
    13741 gtggaattgt tgtctgaata tggtttgttt ttggcgaaaa tcgttaccgt tgtgctagcg
    13801 attgcggcga ttgccgccat tattgtcaat gttgctcaac gtaataaacg ccagcgtggc
    13861 gagttacggg tcaacaatct cagcgaacag tataaggaga tgaaagaaga actggccgcg
    13921 gcgctgatgg actcacatca gcaaaaacag tggcacaaag cgcagaagaa aaagcacaag
    13981 caagaagcga aagcagcaaa agcgaaagcc aaactgggcg aggtggcaac tgacagtaaa
    14041 cctcgcgtct gggtgctgga ttttaaaggc agcatggacg cccatgaagt gaactcgcta
    14101 cgtgaagaga taacggcggt actcgcagca ttcaaaccgc aggatcaggt tgtgctccgt
    14161 ctggaaagcc ctggtggcat ggtgcatggt tacgggttgg cggcttcgca gctgcagcgt
    14221 ctgcgtgata aaaacattcc tttaactgtt acggtagaca aagtcgctgc cagcggcggt
    14281 tacatgatgg cctgtgtggc ggacaaaatt gtttccgcac cgtttgctat tgtgggttcc
    14341 attggggtgg tggcgcaaat gcccaacttt aaccgcttcc tgaaaagcaa agatattgat
    14401 atcgaactgc acactgccgg ggagtataag cgtactctga cgttgctggg tgaaaatacc
    14461 gaagaagggc gggagaaatt ccgcgaagag ttgaacgaaa cgcatcagtt gtttaaagat
    14521 tttgtgaagc gtatgcgtcc gtctctggat attgaacagg tggcaacggg tgaacactgg
    14581 tacggacaac aggcggtaga gaaaggcctg gttgatgaaa tcaacaccag tgatgaagtt
    14641 attcttagcc tgatggaagg ccgtgaagtg gttaatgtac gctatatgca gcgtaaacga
    14701 ctcattgacc gattcaccgg cagcgcggca gagagcgccg atcgattgtt gctacgctgg
    14761 tggcagcggg gtcaaaagcc attgatgtaa aagacaaacg cgaggctaag acctcgcgtt
    14821 ttgctttaat caaccagatg atatttttct gaaagcacat gggccaggtg tttgaacata
    14881 ttaaacaccg cggtgctttt ggctgttggc aatccttgtt catctaaaaa gtagtcgccg
    14941 gtaaatacca gcacgccatt acgctgcgtg acgccggtgg cttcaatccc tgcgagcgta
    15001 tcctcatgct cacgaatgat tttgttggcc tctttcaaca gcgtttcgcg gtcgatgggt
    15061 tgtgtctctt tgttcattat ttactcctta aacaaggaca ttagtctacg ccaggcatgg
    15121 cttgcagaca aatataccac gctggtggca agagcgcctt actggtaact ttggattttg
    15181 catgctaata aagttgcgta tcggatttta tcaggtacag tgtgacgctt tcgtcaatct
    15241 ggcaatagat ttgcttgaca ttcgaccaaa attccgtcgt gctatagcgc ctgtaggcca
    15301 agacctgtta actcagtcac ctgaattttc gtgaacagag tcacgacaag gggttgatat
    15361 ccgcagagaa cgagtccata tcggtaactc gttgccagtg gaaggtttat caacgtgcga
    15421 cgcattcctg gaagaatcaa attaggtaag gtgaatatgg gtaaagctct tgtcatcgtt
    15481 gagtccccgg caaaagccaa aacgatcaac aagtatctgg gtagtgacta cgtggtgaaa
    15541 tccagcgtcg gtcacatccg cgatttgccg accagtggct cagctgccaa aaagagtgcc
    15601 gactctacct ccaccaagac ggctaaaaag cctaaaaagg atgaacgtgg cgctctcgtc
    15661 aaccgtatgg gggttgaccc gtggcacaat tgggaggcgc actatgaagt gttgcctggt
    15721 aaagagaagg tcgtctctga actgaaacaa ctggctgaaa aagccgacca catctatctc
    15781 gcaaccgacc ttgaccgcga aggggaagcc attgcatggc acctgcggga agtgattggt
    15841 ggtgatgatg cgcgctatag ccgagtggtg tttaacgaaa ttactaaaaa cgcgatccgc
    15901 caggcattta acaaagcggg tgagctgaat attgatcgtg ttaatgccca gcaggcgcgt
    15961 cgctttatgg accgcgtggt tgggtatatg gtttcgccgc tgctatggaa aaagatcgct
    16021 cgtggtctgt ctgccggtcg tgtgcagtcg gtggcggttc gcctggtggt cgagcgtgag
    16081 cgtgaaatta aagcgttcgt gccggaagag ttctgggaag tcgatgccag cacgaccacg
    16141 ccatctggtg gagcgttggc gttgcaggtg actcatcaga acgacaaacc gttccgtccg
    16201 gtcaacaaag aacaaactca ggctgcggta agtctgctgg aaaaagcgcg ctacagcgtg
    16261 ctggaacgtg aagacaaacc gacaaccagt aaacctggcg ctccttttat tacctctacg
    16321 ctgcaacaag ctgccagcac ccgtcttgga tttggcgtga aaaaaaccat gatgatggcg
    16381 cagcgtttgt atgaagcagg ctatatcact tacatgcgta ccgactccac taacctgagt
    16441 caggacgcgg taaatatggt tcgcggttat atcagcgata attttggtaa gaaatatctg
    16501 ccggaaagtc cgaatcagta cgccagcaaa gaaaactcac aggaagcgca tgaagcgatt
    16561 cgtccttctg acgtcaatgt gatggcggaa tcgctgaagg atatggaagc agatgcgcag
    16621 aaactgtacc agttaatctg gcgtcagttc gtagcctgcc agatgacccc agcgaaatat
    16681 gactccacga cgctgaccgt tggtgcgggc gatttccgcc tgaaagcacg cggtcgtatt
    16741 ttgcgttttg atggctggac aaaagtgatg cctgcgttgc gtaaaggcga tgaagatcgc
    16801 atcttaccag cagttaataa aggcgatgct ctgacgctcg ttgaacttac accagcccag
    16861 cactttacca agccgccagc ccgtttcagt gaagcatcgc tggttaaaga gctggaaaaa
    16921 cgtggtatcg gtcgtccgtc tacctatgcg tcgatcattt cgaccattca ggatcgtggc
    16981 tacgtgcgag tagaaaatcg tcgtttctat gcggaaaaaa tgggcgaaat cgtcaccgat
    17041 cgccttgaag aaaatttccg cgagttaatg aactacgact ttaccgcgca gatggaaaac
    17101 agcctcgacc aggtggcaaa tcacgaagca gagtggaaag ctgtactgga tcacttcttc
    17161 tcggatttca cccagcagtt agataaagct gaaaaagatc cggaagaggg tggtatgcgc
    17221 ccgaaccaga tggttctgac cagcattgac tgcccgactt gtggtcgcaa aatggggatt
    17281 cgcacagcga gcaccggggt attccttggc tgttctggct atgcgctgcc gccgaaagag
    17341 cgttgcaaaa ccaccattaa cctggtgccg gaaaacgaag tgctgaacgt gctggaaggc
    17401 gaagatgctg aaaccaacgc gctgcgcgca aaacgtcgtt gcccgaaatg cggcacggcg
    17461 atggacagct atctcatcga tccgaaacgt aagttgcatg tctgtggtaa taacccaacc
    17521 tgcgacggtt acgagatcga agagggcgaa ttccgcatta aaggttatga cggcccgatc
    17581 gttgagtgtg aaaaatgtgg ctctgaaatg cacctgaaaa tggggcgttt cggtaaatac
    17641 atggcctgca ccaacgaaga gtgtaaaaac acacgtaaga ttttacgtaa cggcgaagtg
    17701 gcaccaccga aagaagatcc ggtgccatta cctgagctgc cgtgcgaaaa atcagatgct
    17761 tatttcgtgc tgcgtgacgg tgctgccggt gtgttcctgg ctgccaacac tttcccgaaa
    17821 tcgcgtgaaa cgcgtgcgcc actggtggaa gagctttatc gcttccgcga ccgtctgccg
    17881 gaaaaactgc gttatctggc cgatgcgcca cagcaggatc cggaaggtaa taagaccatg
    17941 gttcgcttta gccgtaaaac caaacagcaa tatgtctctt cggaaaaaga cggaaaggcg
    18001 actggctggt cagcatttta tgttgatggc aaatgggttg aaggaaaaaa ataaccttta
    18061 attctgtcag gtttttataa acaaagggtc gcgaaagcgg ccctttttta ttgcatatta
    18121 ttttttcttc acacctatac actaaggcta taaatgatat agtggttata gttagcacct
    18181 tttttattat taaatcgtat tagtcacccg ccaggtgtga cgaaaaaacg atgttctgat
    18241 ggcgtctaag tggatggttt aacatgaaat tacaacaact tcgctatatt gttgaggtgg
    18301 tcaatcataa cctgaatgtc tcatcaacag cggaaggact ttacacatca caacccggga
    18361 tcagtaaaca agtcagaatg ctggaagacg agctaggcat tcaaattttt tcccgaagcg
    18421 gcaagcacct gacgcaggta acgccagcag gacaagaaat aattcgtatc gctcgcgaag
    18481 tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggtga gcacacctgg ccggataaag
    18541 gctcgctgta tatcgccacc acgcataccc aggcacgcta cgcgttacca aacgttatca
    18601 aaggctttat tgagcgttat ccccgcgttt ctttgcatat gcaccagggc tcgccgacac
    18661 aaattgctga tgccgtctct aaaggcaatg ctgatttcgc tatcgccaca gaagcgctgc
    18721 atctgtatga agatttagtg atgttaccgt gctaccactg gaatcgggct attgtagtca
    18781 ctccggatca cccgctggca ggcaaaaaag ccattaccat tgaagaactg gcgcaatatc
    18841 cgttggtgac atataccttc ggctttaccg gacgttcaga actggatact gcctttaacc
    18901 gcgcagggtt aacgccgcgt atcgtcttca cggcaacgga tgctgacgtc attaaaactt
    18961 acgtccggtt agggctgggg gtaggggtca ttgccagcat ggcggtggat ccggtcgccg
    19021 atcccgacct tgtgcgtgtt gatgctcacg atatcttcag ccacagtaca accaaaattg
    19081 gttttcgccg tagtactttc ttgcgcagtt atatgtatga tttcattcag cgttttgcac
    19141 cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt gcgctctaat gaagaaattg
    19201 aggtcatgtt taaagatata aaactgccgg aaaaataatt tcttgcgtta ttttcggcac
    19261 cttttatgta gcgaaggtgc cggaatatat tctcttttgt tacttatttc tataccgtgc
    19321 acaatatttt tatccctcat atttataggg taaattacct gtaaagtagt gtctaattac
    19381 ccgcctcgca aactccagtt ttcaaatatc tatttcaggt caaaagattg aattattcac
    19441 tggagacgat tcgtaaattc gctggatttt tgactaaagt ttctttagga tttgtctcat
    19501 cgtatgatta attacaccaa tcgttcaacg tcttgctgat aagtgatggc gattatatga
    19561 ggttagcaat gccttctgga aatcaggaac cccgccgcga tcctgaatta aaacgtaaag
    19621 cctggctggc ggtttttctt ggttctgcac ttttctgggt ggttgtcgca ctgctgattt
    19681 ggaaagtgtg gggataacta tggtcggtca ggagcaactg gagtcgtcac cattatgcca
    19741 gcatagtgac aatgaaacag aaacgaaacg ggaatgttcc gtcgttattc cagacgactg
    19801 gcaactaaca tcgcagcagc aagcctttat agaactgttt gctgaagatg atcagccgaa
    19861 acaataatta tcatcattct tattacccat ttttaatgaa ttaaagggct tttaatacac
    19921 cgcagcaata acagcttgag ttatctcaac acaaaataat aaccgttaag ggtgtagcct
    19981 atgatcaaca caaatatgaa atattggtcc tggatgggcg cgttttctct gtcgatgctc
    20041 ttctgggccg aactcctctg gatcattact cactgatcct tgaccccgct gcggcggggt
    20101 tgtcatttgc tttgccacaa ggtttctcct cttttatcaa tttgggttgt tatcaaatcg
    20161 ttacgcgatg tttgtgttat ctttaatatt caccctgaag agaatcaggg cttcgcaacc
    20221 ctgtcattaa ggaggagcta tgtcgtcaac cctacgagaa gccagtaagg acacgttgca
    20281 ggccaaagat aaaacttacc actactacag cctgccgctt gctgctaaat cactgggcga
    20341 tatcacccgt ctgcccaaat cactcaaagt tttgctcgaa aacctgctgc gctggcagga
    20401 tggtaactcg gttaccgaag aggatatcca cgcgctggca ggatggctga aaaatgccca
    20461 tgctgaccgt gaaattgcct accgcccggc aagggtgctg atgcaggact ttaccggcgt
    20521 acctgccgtt gttgatctgg cggcaatgcg cgaagcggtt aaacgcctcg gcggcgatac
    20581 tgcaaaggtt aacccgctct caccggtcga cctggtcatt gaccactcgg tgaccgtcga
    20641 tcgttttggt gatgatgagg catttgaaga aaacgtacgc ctggaaatgg agcgcaacca
    20701 cgaacgttat gtgttcctga aatggggaaa gcaagcgttc agtcggttta gcgtcgtgcc
    20761 gccaggcaca ggcatttgcc atcaggttaa cctcgaatat ctcggcaaag cagtgtggag
    20821 tgaattgcag gacggtgaat ggattgctta tccggataca ctcgttggta ctgactcgca
    20881 caccactatg atcaacggcc
//
LOCUS       DAZFYC010000081        20811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFYC010000081 DAZFYC010000000
VERSION     DAZFYC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.081"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(116..1765)
                     /gene="actP"
                     /locus_tag="ACHKW5_003361"
     CDS             complement(116..1765)
                     /gene="actP"
                     /locus_tag="ACHKW5_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO7512697.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATKREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1762..2076)
                     /gene="yjcH"
                     /locus_tag="ACHKW5_003362"
     CDS             complement(1762..2076)
                     /gene="yjcH"
                     /locus_tag="ACHKW5_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO7512698.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVLSFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2276..4234)
                     /gene="acs"
                     /locus_tag="ACHKW5_003363"
     CDS             complement(2276..4234)
                     /gene="acs"
                     /locus_tag="ACHKW5_003363"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO7512699.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4626..6062
                     /gene="nrfA"
                     /locus_tag="ACHKW5_003364"
     CDS             4626..6062
                     /gene="nrfA"
                     /locus_tag="ACHKW5_003364"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO7512700.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIEIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6107..6673
                     /gene="nrfB"
                     /locus_tag="ACHKW5_003365"
     CDS             6107..6673
                     /gene="nrfB"
                     /locus_tag="ACHKW5_003365"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO7512701.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6670..7341
                     /gene="nrfC"
                     /locus_tag="ACHKW5_003366"
     CDS             6670..7341
                     /gene="nrfC"
                     /locus_tag="ACHKW5_003366"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO7512702.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLMIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7338..8294
                     /gene="nrfD"
                     /locus_tag="ACHKW5_003367"
     CDS             7338..8294
                     /gene="nrfD"
                     /locus_tag="ACHKW5_003367"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO7512703.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8314..10032
                     /gene="nrfE"
                     /locus_tag="ACHKW5_003368"
     CDS             8314..10032
                     /gene="nrfE"
                     /locus_tag="ACHKW5_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO7512704.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10025..10408
                     /gene="nrfF"
                     /locus_tag="ACHKW5_003369"
     CDS             10025..10408
                     /gene="nrfF"
                     /locus_tag="ACHKW5_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO7512705.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10405..11001
                     /gene="nrfG"
                     /locus_tag="ACHKW5_003370"
     CDS             10405..11001
                     /gene="nrfG"
                     /locus_tag="ACHKW5_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO7512706.1"
                     /translation="MKQSQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAQYQRQRD
                     PLHQFASQQTPEAQLQVLQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11343..12656
                     /gene="gltP"
                     /locus_tag="ACHKW5_003371"
     CDS             11343..12656
                     /gene="gltP"
                     /locus_tag="ACHKW5_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO7512707.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTIAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12734..13423)
                     /gene="yjcO"
                     /locus_tag="ACHKW5_003372"
     CDS             complement(12734..13423)
                     /gene="yjcO"
                     /locus_tag="ACHKW5_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO7512708.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13517..15664)
                     /gene="fdhF"
                     /locus_tag="ACHKW5_003373"
     CDS             complement(13517..15664)
                     /gene="fdhF"
                     /locus_tag="ACHKW5_003373"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15245..15247),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO7512709.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15862..17328)
                     /gene="mdtP"
                     /locus_tag="ACHKW5_003374"
     CDS             complement(15862..17328)
                     /gene="mdtP"
                     /locus_tag="ACHKW5_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO7512710.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17325..19376)
                     /gene="mdtO"
                     /locus_tag="ACHKW5_003375"
     CDS             complement(17325..19376)
                     /gene="mdtO"
                     /locus_tag="ACHKW5_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO7512711.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEVRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19376..20407)
                     /gene="mdtN"
                     /locus_tag="ACHKW5_003376"
     CDS             complement(19376..20407)
                     /gene="mdtN"
                     /locus_tag="ACHKW5_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO7512712.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20426..20701)
                     /gene="ytcA"
                     /locus_tag="ACHKW5_003377"
     CDS             complement(20426..20701)
                     /gene="ytcA"
                     /locus_tag="ACHKW5_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO7512713.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4689 a         5509 c         5701 g         4912 t
ORIGIN      
        1 ctgtcaaaaa tcgctcattt tttaatgagt ttatttgttt aatattatgg gaaaaggtga
       61 tgcatgttgg agaggaagag tattccccgg tcaggcgacc ggggaagggg agagattaat
      121 gtgcgcggcc ttgctcaacg ccaaagccgg tctgggagcg gataaactgt gcacggaaca
      181 gttcacgctc gcgcgcacct tccgctgagt tatcggttgc cgagaagaac cagatgccga
      241 ggaatgccac gctgatagag aacagcgccg ggtattcata cgggaagatg gctttttcgt
      301 gaccaaggat ctgtacccaa atcgtcgggc cgaggatcat cagtaccact gcggtaatca
      361 gccccagcca gccacccatc atcgcgccac gagtggtcag tttcgaccag tacatagaaa
      421 gcagaatgat cgggaagtta cagctcgctg cgatggcaaa cgccaggcct accatgaagg
      481 cgatgttctg attctcaaac agcacgccga ggataatggc aatcacacct aaaatcagca
      541 cggtgatttt cgatacccgc agttcttcac gtttggtcgc gccttttttg aagacgttag
      601 cgtacaggtc atgcgaaacc gctgatgcgc ccgccagcgt cagaccagca accaccgcaa
      661 gaatggtggc aaaagcaacc gctgaaataa aaccgaggaa caggttgccg cccactgcat
      721 tcgccaggtg aacggccgcc atgttgttac caccaatcag atggcccgcc gcgtctttat
      781 attccggatt cgcaccaacc agcatgatcg cgccgaagcc gataataaag gtcagaatat
      841 agaagtagcc cataaacccg gtggcgtaga acacgctctt acgtgcttcg cgggcatcgc
      901 tgactgtaaa gaagcgcatc agaatgtgcg gcaagcccgc cgtaccaaac atcagtccca
      961 gacccagaga gagcgcggag atcggatctt tcaccagccc gcccggcttc atgatgtcga
     1021 cacctttcgg gtgtaccgcc atcgcttcac tgaacagatt gttgaagcta aagccgacgt
     1081 gtttcatcac cataaaggcc ataaagctgg caccgaacag caacagcacg gctttgataa
     1141 tctgtaccca agtggtcgcc agcatgccgc cgaacaggac gtacatcatc atcagcacgc
     1201 cgaccagcac caccgcaatg tgatagttaa ggccaaacag cagctcgatc agtttacctg
     1261 cgcccaccat ctgggcgata aggtaaagcg ccaccaccac cagagaacca caggccgaaa
     1321 gaatacgaat cggcccttgt ttcagacggt aagaggccac atcggcaaag gtgtagcgcc
     1381 ccaggttacg cagacgttcg gcaatcagga acaaaatgat cggccagccc accaggaagc
     1441 ccagcgagta aattaagccg tcatagccgg aggtaaacac cagcgcggag atccccaaga
     1501 atgaggcggc ggacatatag tccccggcaa tcgccagccc gttctggaag ccagtgatat
     1561 tgccgcctgc ggtgtagtag tcgctacgag aacgtacgcg ttttgatgcc cagtaggtaa
     1621 tgccgagcgt aaacacgacg aaaatcagga acataataat cgcctgccag ttcgttggct
     1681 ggcgctctac ggccccgcta atagcatccg cggcgttagc tgcgaaaggg agtgtggcgg
     1741 caagcgccgt cagaactctt ttcatgatgc ttgtacctca tgcaggactt cattattaag
     1801 acggtcgaat tcgccgttcg cccgccagat gtagataccg gtgagaacaa aggagagcac
     1861 aatcactcca acaccaattg gaataccgcg tgtgacgctg gtgttcggat tcagcggcgt
     1921 gcccagccag ccgggcgcga aggcgatcag taaaataaag ccgatataaa ctgccagcat
     1981 aataatcgac aggatggtgg caaaccgttg ccgtttttcg actaactccc tgaaatgcgc
     2041 attgtcttct atccgctgat aaatagtgcc attcatcaca gattctccag aggtaatgta
     2101 gggattgttt taattcccgc tcccttatgg gagaaggtta acgctcgggt aacccttgcc
     2161 gaatgtaggc cggataaggc gtttacgccg catccggcaa tcaatgcctg atgcgacgct
     2221 gtcgcgtctt atcaggccta caaaccgtta ccgactcgca tccggcaatt gtgggttacg
     2281 atggcatcgc gatagcctgc ttctcttcaa gcagcttctc gactacgcca ggatcggcaa
     2341 gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc aattttgcgc agaatacggc
     2401 gcataatttt gccggagcgg gttttaggca gggagtcggt ccagtgcagc acgtctggcg
     2461 tcgccagcgg gccaatctct ttacgcaccc agttgcggac ttctgcgtac agttctggtg
     2521 acggttcctc cccgtgatta agcgtgacgt aggcgtagat cgcctgacct ttaatattgt
     2581 gcggaatacc tactacggcg gcttcggcaa tcttcggatg cgccaccagc gccgactcaa
     2641 tctctgccgt ccccagacgg tgaccggaga cgttcagcac gtcgtccaca cgcccggtga
     2701 tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc gctgaaatac atatttttga
     2761 aggtggagaa gtaggtctgt tcaaaacgtt cgtgatcgcc aaacagcgta cgcgcctgac
     2821 ccggccagga gtcggtgatc accaggctgc cttcggtagc gccttccagc gggttacctt
     2881 cgttatcgac cagcgccggt tgcacgccga agaacggacg tgttgccgaa ccggctttca
     2941 gctcggtagc gccaggcagc ggcgtgatca tgaaaccgcc agtttcggtc tgccaccagg
     3001 tatcgaccac cggacatttc tcgttgccga tttttttcca gtaccactcc cacgcttccg
     3061 ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga cgaacggtcg gtgccttcga
     3121 tcgctttatc gccttccgcc atcagcgcgc ggatcgccgt gggtgcggta tagagaatat
     3181 tgacctgatg cttgtccacc acctgcgcca tacgggcagg cgtcggccag ttgggtacgc
     3241 cttcaaacat cagcgtggtc gcaccgcagg ccagcgggcc gtacagcaag taactgtgtc
     3301 cggtcaccca gcccacatcg gcggtgcacc agtagatatc acccggatga taatcaaaga
     3361 catatttaaa ggtcagcgcc gcgtacacca gataaccgcc ggtagtatgc agcacacctt
     3421 ttggcttacc ggtagaaccg gaggtgtaga gaataaacag cggatcttcg gcgttcatct
     3481 cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc gtgccaccac aggtcgcgcc
     3541 cttcctgcca gtcaattttc ccgccagtac gcttcagtac caccacatgc tctacgctgg
     3601 tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt cagcggaata ctgcgcccgg
     3661 cacgcacacc ttcgtcggaa gtgatcacca gtcgtgagtt ggaatcaata atgcgcccgg
     3721 caacggcttc cggcgagaag ccgccgaaaa tcaccgaatg caccgcgcca atgcgggcgc
     3781 aggccagcat cgcaaccgcg gcttccggca ccatcggcat ataaatcgcc accacatcac
     3841 cttttttaat gcccagctcg agcagggtat tggcgaagcg gcagacgtcg cggtgcagct
     3901 ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc ttcccagatg atggcggtgc
     3961 gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc cgccagattc agcgtgccgt
     4021 cctcgtacca tttaatggac acattaccgg gggcaaagga ggtatttttc accttctggt
     4081 acggtttgat ccagtcgaga atttttccct gttcgcccca gaaggtatca ggtacgttaa
     4141 tagattgttg atacatcgcc tcgtactgct gagggtttat caggcaacgg tctgcgatgt
     4201 tggcaggaat ggtgtgtttg tgaatttggc tcatgctttt gttctccttg tgggatgtta
     4261 ataatatgtg gcataagcgt taaatgtagg ggtgttggca gttttgttta gtatttgggc
     4321 gacagatcac gcaaaagtag aattgtgcaa ataaacggca gggtaatttt tgaaggtcac
     4381 caacaaaagt tgattaattc tttgaggaac atgcagttat gcatgctgtt gaaaaagagg
     4441 aagatactga ctaactctaa agtggtattt tacatgcact tacaattgat taaagacaac
     4501 attcacagtg tggttatttg ttacacatag gggcgagcaa tgtcatgaca gtgtaggtgc
     4561 agttactgtc gtgaaaacaa taaaaaccgc cattgcaaca atggcgcaat tcggatgaag
     4621 cccctatgac aaggataaaa ataaacgcac gccgtatctt cagcttattg attccttttt
     4681 tctttttcac ttctgttcac gctgaacaaa cggcagctcc cgcaaaacct gtaactgtgg
     4741 aagcgaagaa tgaaaccttt gccccgcagc atcccgatca atatctctcc tggaaagcca
     4801 cctcggaaca gtcagagcgt gttgacgccc tggcggaaga tccacggctg gtgatcctgt
     4861 gggcggggta tcccttctcg cgcgattaca acaagccgcg tggacatgcc tttgctgtga
     4921 ccgatgtgcg tgaaaccctg cgtaccggtg cgccgaaaaa cgctgaagat ggtccgctac
     4981 cgatggcgtg ctggagttgt aaaagcccgg atgtggcgcg tctgatccag aaagacggcg
     5041 aagatggcta cttccacggt aagtgggcgc gcggcggccc ggaaatcgtc aacaacttag
     5101 gttgtgccga ctgccataac accgcctcac cagagttcgc caaaggcaaa ccggagttaa
     5161 ccctttcccg tccgtatgcg gctcgcgcga tggaagccat tggtaaacct tttgagaaag
     5221 ccggacgttt cgaccagcaa tcgatggttt gcggtcagtg ccatgtggag tattacttcg
     5281 acggcaaaaa caaagcggtt aaattcccgt gggatgacgg catgaaagtc gaaaatatgg
     5341 agcagtatta cgacaaaatt gccttctctg actggactaa ctccctgtcg aaaacgccaa
     5401 tgctgaaagc gcagcacccg gaatatgaaa cctggacagc gggcattcac ggtaaaaaca
     5461 acgtgacctg tatcgactgc catatgccaa aagtgcagaa cgccgaaggc aaactctaca
     5521 ccgaccataa aattggtaat ccgtttgata acttcgccca gacttgtgcg aactgccata
     5581 cccaggacaa agctgccttg caaaaagtgg tcgcggaacg taagcagtcg attaacgacc
     5641 tgaaaatcaa ggttgaagat caactggttc acgctcactt cgaagcgaaa gcagcgctgg
     5701 atgcaggcgc gacggaagcc gaaatgaagc cgattcagga cgatatccgt catgcccagt
     5761 ggcgttggga tctggcgatc gcttcccacg gcattcatat gcacgcaccg gaagaaggtt
     5821 tacggatgct cggtactgcg atggataaag cggcggatgc acgcaccaaa ctggcgcgcc
     5881 tgctggcgac caaaggcatc acccatgaaa tcgagatccc ggatatctcg accaaagaga
     5941 aagcccagca ggccattggc ctgaacatgg aacaaatcaa ggccgagaag caggacttca
     6001 tcaaaacggt gatcccgcag tgggaagaac aggcacgtaa aaatggtctg ttaagccaat
     6061 aaccccgttc cgactcgcaa ggggcggaaa acacaatgga gtgaatatga gcgtattacg
     6121 ttcgttatta actgctgggg tgctggcgtc gggcctgttg tggagcctga acggaattac
     6181 cgctacccct gcggcgcagg catctggtga tcgctacgaa gttacccagc agcgtaaccc
     6241 ggatgccgcc tgtctggact gtcataagcc agatatcgaa ggtatgcatg gcaaacatgc
     6301 ttccgttatc aacccgaata acaaactgcc ggtcacctgc accaactgtc acggccagcc
     6361 atcaccgcaa caccgcgaag gggtgaaaga tgtgatgcgc tttaacgagc cgatgtacaa
     6421 ggtcggagag cagaacagcg tctgtatgtc ctgtcatctg ccggaacagt tgcaaaaagc
     6481 gttctggccg cacgatgtcc acgtaaccaa agtggcgtgc gccagctgcc attccctgca
     6541 tccacagcaa gatacgatgc agacgttaag tgacaaagga cggattaaga tttgcgtcga
     6601 ttgccacagc gatcagcgca ccaatccgaa ctttaacccg gcgtctgttc cgttgcttaa
     6661 ggagcagcca tgacctggtc tcgtcgccag tttctcaccg gcgtcggcgt gctggcagcc
     6721 gtcagcggca ccgcagggcg cgtggtggcg aaaacgttga atatcaatgg ggtgcgttac
     6781 ggcatggtgc atgacgagtc gttatgcatc ggctgtaccg cctgtatgga tgcttgtcgg
     6841 gaagtgaaca aggtgccgga aggcgtctcg cgcctgatga ttattcgtag cgagccgcag
     6901 ggcgaatttc ctgatgtgaa atatcgcttc ttccgtaagt cttgccagca ctgcgatcat
     6961 gcgccgtgcg ttgacgtctg cccgaccggt gcgtcttttc gtgatgccgc tagcgggatt
     7021 gtcgatgtta atcctgatct ctgcgtcggt tgtcagtact gcatcgccgc ctgtccgtac
     7081 cgcgtgcgct ttatccatcc ggtcacgaag acggcggaca aatgcgattt ctgccgtaag
     7141 accaatttgc aggccggtaa gctgcccgcg tgcgttgagg cttgcccgac gaaggcgctg
     7201 acgtttggca atctggacga tcctaacagt gagatttcgc aactgctgcg ccagaagcct
     7261 acttaccgct acaagctggc gctgggaacc aaaccgaagc tgtaccgcgt accgtttaaa
     7321 tacggggagg tgagccaatg acgcagactt ccgcatttca ttttgaatcg ctggtgtggg
     7381 actggccgat tgccatctac ctgtttttga ttggtatttc tgccggtctg gtgacgctgg
     7441 ccgtgctgtt acgtcgcttc tacccgcagg cgggcggtgc agacagcacg ttgctgcgca
     7501 ccacgctgat tgtcggacct ggcgcggtga tcctcggtct gttgatcctc gtcttccacc
     7561 tgacaagacc gtggaccttc tggaagctga tgttccacta cagttttacc tcggtgatgt
     7621 cgatgggggt gatgctgttt cagctctaca tggtggtgct ggtgctgtgg ctggcgaaaa
     7681 tctttgaaca tgatttgctt gccctgcaac aacgctggtt gccgaagctg gggatcgtgc
     7741 aaaaggttct gagcctgctg acgcccgttc atcgcggact ggaaacgctg atgctggtgt
     7801 tggcggtgct gctgggggct tataccggct ttctgctgtc ggcgctgaaa tcgtatccgt
     7861 tcctcaataa cccgatcctg ccggtgctgt tcctcttctc cggcatctcg tccggtgtgg
     7921 cggtagcgct gatcgccatg gcgatacgcc aacgcaaaaa tccgcattcc acggaagcgc
     7981 actttgttca ccgtatggaa atccccgtgg tatggggtga aatcttcctg ctggtggcgt
     8041 tttttgtcgg tctggcgctg ggcgatgacg gtaaagtgcg tgcgctggtg gcggcattag
     8101 gtggcggttt ctggacgtgg tggttctggc ttggtgtcgc cgggctgggg ctgattgtgc
     8161 caatgttgct caaaccgtgg gtcaatcgca gttccggcat tcctgccgtg ttggcggcgt
     8221 gtggggccag tctggtcggc gtgttgatgc tgcgcttttt cattctctac gccggacagt
     8281 tgacggtggc gtaagccaga aaagaggtgg tttctggacg tattccttcc tgaagtcggt
     8341 tttctggcgt tgttgttaag tctcggggtc aacgtgttga ccccgttgac ggcctttgcg
     8401 ggagtgcggt tgcgctggcc tgccatgatg cgactcactt gcatcggcat tctggcgcag
     8461 ttcgcgctcc tgctgctcgc ctttggcgta ctgacgtatt gttttctcat cagcgatttc
     8521 tcggtcattt atgtcgcgca acatagctat agcctgctgt cgtgggaact caagctggca
     8581 gcggtgtggg gcggccatga aggttcgctg ctgctttggg tgctgctgct ttccgcctgg
     8641 agcgcgctgt ttgcctggca ttatcggcag caaaccgatc cgctatttcc gctgacgtta
     8701 gccgttttat ctctcatgct cgccgcactg ctactgtttg tggtgctgtg gtccgatccc
     8761 ttcgtgcgga tatttccacc agcaatcgaa ggccgcgatc tcaatccgat gctgcaacat
     8821 cccggtctta tctttcatcc accgctgctt taccttggct atggcggttt gatggtagcg
     8881 gcgagcgtgg cgctggcgag tttactgcgc ggcgagtttg atggtgcctg cgccagaatt
     8941 tgttggcgct gggcactacc tggctggagc gcattaacgg cggggatcat cctcggttcc
     9001 tggtgggcct attgcgaact gggctggggc ggctggtggt tctgggatcc ggtggaaaac
     9061 gcctctttat taccctggct ttctgccact gcgctgctgc acagtttgtc cctgacacgc
     9121 cagcgggaga ttttccgcca ctggtcgctg ttgctggcga tagtaactct gatgctgtcg
     9181 ctactgggta ccttaattgt ccgttctggc attctggttt cggttcatgc gttcgcactg
     9241 gataacgtcc gcgccgtgcc gttgttcagc ctgtttgcac tgattagcct tgcgtctctg
     9301 gctctgtatg gctggcgagc gcgggacggt ggcccggtgg tgcgtttttc ggggttatcg
     9361 cgggaaatgt taatcctcgc tacgctgttg ctgttttgcg cagtgctact gatcgtgctg
     9421 gtgggaacgc tttatccgat gatttacggt ctgctgggct ggggacgcct ctccgttggc
     9481 gcgccgtatt ttaaccgcgc gacgttaccg tttggcctgt tgatgctggt ggtgattgtg
     9541 ctggcgacgt ttgtctctgg caaacgcgtg cagcttccgg cgctggtagc tcatgcgggc
     9601 gtgctgttat ttgccgctgg gatcgtggtt tccagcgtca gccgtcagga gatcagcctg
     9661 aatttacagc cgggtcagca ggtgacgctg gcaggataca ccttccgttt tgagcgcctc
     9721 gatctgcaag ccaaaggcaa ttacaccagc gaaaaagcga tagtggcact gtttgaccat
     9781 cagcaacgca ttggtgaact gatgccggag cggcgttttt acgaagcacg tcgtcagcaa
     9841 atgatggaac cgtcaattcg ctggaacggc atccatgact ggtatgcggt catgggtgaa
     9901 aaaaccggag cggatcgtta cgcttttcgc ttgtatgtac aaagcggtgt gcgctggatc
     9961 tgggggggag gattgttgat gattgcgggc gcattattaa gcggatggcg ggggaggaag
    10021 cgcgatgaat aaaggacttc tcacgctact gctgttattt acctgttttg cgcgcgctca
    10081 ggttgtagac acctggcaat tcgccaatcc gcaacaacag caacaggcgt taaatattgc
    10141 cagccagtta cgttgtccgc agtgccagaa tcagaactta ctggaatcca atgcaccggt
    10201 ggcggtcagt atgcgccatc aggtttacag catggtggcg gaggggaaga acgaagtcga
    10261 aatcattggc tggatgaccg aacgctacgg agattttgtt cgctataacc caccgttaac
    10321 gggtcagacg ctggtgttat gggcgctgcc agtggtgttg ttactgctga tggcactgat
    10381 cctctggcga gtgagggcga agcggtgaaa cagtcacaaa taccggtgaa aatgctgaca
    10441 acactcacga ttttgatggt atttctctgt gtcggcagtt atctgttaag cccaaaatgg
    10501 caggcggtac gtgcgcaata tcagcgtcag cgcgatccgc tacatcagtt tgccagccag
    10561 caaaccccgg aagcgcagct tcaggtattg caggataaaa tccgcgctaa tccacaaaac
    10621 agcgaacagt gggcgttact gggcgagtac tatctgtggc aaaacgatta cagtaattcg
    10681 ctgctggcgt atcgtcaggc gttgcaacta cgtggtgaga acgctgaact gtatgcggcg
    10741 ctggcgacgg tgctttatta ccaggccagc cagcatatga ccgcccagac tcgcgcaatg
    10801 atcgacaaag ccctcgcgct ggactctaac gaaatcaccg ctctgatgct gctggcttcc
    10861 gatgcgttta tgcaggcgaa ttacgcgcaa gccatcgaac tatggcaaaa agtgatggat
    10921 ctcaactcac cccggatcaa ccggacacag ctggtcgagt cgattaatat ggcgaaattg
    10981 ttgcagcgga gatcggatta atcatctggt gatgaagatt atgttattga gctgcataaa
    11041 aaataatcga atgaacatat gccaaaaata atcaccaatc agtattattg cagattaaac
    11101 aaataaaaat ctttccataa caaatggtta ttcattaatc ctgccataaa ctcgcgttat
    11161 tatgcattaa tgcagcgaaa agctctgttg ttaaagggtt gcgcaacata ccgcgcaaat
    11221 gatactgatc ataagcgtta aaaaaatcta caaaccaacg caacacaatt catgccctag
    11281 cagtatgtca cgttctcgcg tttctgaacg gggaacggcg ctccattgag gaagttattc
    11341 tgatgaaaaa tataaaattc agcctggcct ggcagattct gtttgctatg gtgctgggca
    11401 ttctcctggg aagctacctg cactaccata gcgacagccg cgactggctg gtcgtcaatt
    11461 tgctctctcc ggcgggtgat atcttcatcc atctgattaa aatgatagtt gtgccgattg
    11521 tgatctccac gctggtggtg ggtatcgcgg gtgttggtga tgccaaacag ctcgggcgta
    11581 ttggcgcgaa aaccattatc tacttcgagg tgatcaccac catcgctatc attttgggga
    11641 tcactctggc gaacgtcttc cagcccggtg ccggggtgga tatgtcgcag ctggcgaccg
    11701 tcgatatctc gaaatatcag agcactacgg aagcggtaca aagcagttcc cacggcatta
    11761 tgggcacgat tttgtcgctg gtgccgacga acattgtggc gtcgatggcg aaaggcgaaa
    11821 tgctgccgat catcttcttc tcggtgctgt ttggtctggg gctttcctcc ctgcccgcga
    11881 cgcatcgtga accgctggtg accgtgttcc gctccatctc tgaaaccatg tttaaagtga
    11941 ctcacatggt gatgcgttat gcaccggtgg gtgtgtttgc gctgattgcg gtgacggtgg
    12001 ctaactttgg tttctcttct ctgtggccac tggcgaaact ggtgctgctg gtgcatttcg
    12061 ccattctgtt cttcgcgctg gtagtgctgg gaattgtggc gcgcctgtgc gggttaagcg
    12121 tctggatcct gattcgtatt ctgaaagatg agctgattct ggcgtactcc actgccagct
    12181 ctgaaagcgt actgccgcga attattgaga agatggaagc ctacggagca ccggcgtcga
    12241 tcaccagctt cgtggtgccg accggttact cttttaacct tgatggttcg acgctgtatc
    12301 aaagtattgc cgctattttc atcgctcagc tgtatggcat tgacctgtcc atctggcagg
    12361 aaatcattct ggtgctgacg ctgatggtga cctcgaaagg gattgctggc gtgccgggcg
    12421 tgtcgtttgt ggtgttgctg gcaacgctgg gtagcgtagg tattccgctg gaaggcctgg
    12481 cgtttattgc tggtgttgac cgtatcctcg acatggcgcg tactgcgctg aacgtggtgg
    12541 gtaatgcgct ggcggtgctg gtgattgcca agtgggaaca caaatttgac cgcaaaaagg
    12601 cgctggctta tgagcgtgaa gtgctgggca aatttgataa aactgcggat caataattga
    12661 agattgccgg ggttatccac ccggcaatgt gtggatgcct gatgcgacgc tggtgcgtct
    12721 tatcaggcct accttaaccg ttggttaatt tttcaaactc ttcacacccg gtatcaaacc
    12781 cttccataca gctcaggttc aaccagtgca gcgccttttg tttgttcttc tcgataaaac
    12841 cttcctcacc gtttaagaac atcattcccg cccagtactc ggaataaccg gttcgggaaa
    12901 ttgcagagct gcgtttgaaa taccaggttg ccttgtcatc gtcggcctta atgcccacgc
    12961 cgttggcgta aatcaaacca agcagcattt gggcatcgac ggcagagtcg ttctccagat
    13021 cttccgaggc gttttctaac agcgaaatcg cctttggata atccggttta cccgcttgag
    13081 tatttaccag aatatgcgcc agcgttactt caccttcttt actgcccgct tgcgccgctt
    13141 tctctgcaag aacttttgct tgtggatagt ccagactgac cggattggtg attttgatct
    13201 gcgccagcag cgcgcaggca tcagcatcac cgctgtcggc ggctttctgt gcccaatatt
    13261 cggctttgct caaatcgccg gagctaaacc agctgtcggc aagaaaatat tgtgcgcgtc
    13321 ggtccccggc ctctgctgcc tttaaatact ggctgccagg ctcggagtcg ttcgcgtggg
    13381 caaagaatgt caaaaacaac atcaatgcga taattttttt cattttgata atacgattgg
    13441 gtagggagta accagtataa agagttcgaa tggataaaaa aacagcctcc gaaaggaggc
    13501 tgtagaaagg actgtattac gccagtgccg cttcgcgcag gcgagttttc aacttgttgt
    13561 actcgtcaat cacgtactgc tcggcggcgc gctgatcggc gatcggctcg acgcgcacgg
    13621 cgcagtattt gtattccggc gttttcgtaa tcgggcttaa gttttcggta accagctcgt
    13681 tacaggcacc aatccaccac tggtaggtca tgtaaatcgc ccctttgttc ggacgatcgc
    13741 tgacctgcgc acgggtgata attttgcctt tacgcgagtg cacccaaacc aatgcctcat
    13801 cttcaatacc cagacgtttg gcgtcttcgg tattgatttg tgcgtagcca ggttcatcag
    13861 ccagcgccgc cagtgccgca cagttaccgg tcatcgaacg gcaagagtag tgaccaactt
    13921 cacgcaccgt tgacagtacc atcgggtact cgtcggtgag tttgtcgatt ggcgctaccc
    13981 agtcgcaggt gaagaactgc gccagaccgt tcggggtatc aaacttctct ttaaacagat
    14041 aagaagtccc ctgatcggca tctgaagtat cgcggcaagg ccactgaatg aagcccagtt
    14101 cgcccatttt ctcgtaagtc gcaccgtaga aatccgggca cagatgacgc aactcatccc
    14161 agatctcctg ggtgttgttg tagtgcatcg gataacccat acgggtggcg atttcactga
    14221 tgatttgcca gtccgttttc agatcccatt tcggttcaac tgctttgaag aaacgctgga
    14281 agccacggtc agccgcagta aacacgcctt catgctcgcc ccacgacgtt gacggtaaaa
    14341 taacatccgc cgccgacgcg gttttggtca taaagatgtc ctgaacgata accagttcca
    14401 gatcttcaaa ggctttacgt actgccgaca gctccgcgtc agtttgcagc ggatcttcgc
    14461 ccataatgta cgcggcacgc acttcgccat gcgctgcgcg gtgcggcagc tcgctgatgc
    14521 gataaccggt atgagcaggc aggctttcca cgccccaggc tttggcgaat ttctcgcggt
    14581 tagccggatc tttcacgtac tggtatcccg gatacgtatc cggcagcgcg cccatatcgc
    14641 aggcaccctg cacgttgttc tgaccacgaa ccgggtttac acccgcatgc ggcttaccga
    14701 ggttaccggt cagcatcgcg aggctggtca gagaacgcac ggtttccacg ccctggtaga
    14761 actgggttac acccatgccc cacaggatgg cggcgctttt cgcctgggca tacatccgtg
    14821 ccgcctgacg aatctcactg gcgctgacgc cggtgatatc ttcaaccgac tccggcgtgt
    14881 agccttcaac gattttacga tactcttcaa agccttctgt acgggaagcg acgaacgctt
    14941 tgtcgtacag attttcttca ataatgacat ggcccatcgc attcaacagc gcgatgttcg
    15001 agccgttttt cagtgcaatg tgcatgtcag caatgcgcgc ggtttcaatt ttgcgcggat
    15061 cgcagacgat aattttcgcc ccgttacgtt tagcgttaat tacgtgattc gccacgattg
    15121 ggtgggaatc cgccgggttg tacccgaaaa cgaacactaa atcggtatta tcaatttcgt
    15181 taatagcatt gctcattgcg ccattaccga ccgattggtg cagacctgca accgatgggc
    15241 cgtgtcagac acgagcgcag cagtcaacgt tattggtacc aataacggcg cgcgcaaatt
    15301 tttgcattac atagttggtt tcgttacccg taccacgcga ggagccggtc gtctggatgg
    15361 catccggacc gtacttctct ttgatggcgc tcaggcgctc ggcaacgtaa ttcagtgcct
    15421 catcccagga aacaggttcg agtttgccgc cacgctggcg acggatcatg ggggttttca
    15481 ggcgcggggt caggatctgg gtatcgttaa tgaaatccca gccataataa cccttcagac
    15541 acagggtacc ctggttggtt ttcccctgcg ctgcctccgc ccggacgatt ttgccgttat
    15601 cgacgaccag gttgattttg caacctgatg cgcaataggg gcaaaccgtg acgacttttt
    15661 tcatcggtct cgctccagtt aatcaaatca cgcatacgcg ctctcgacta cagtatgcat
    15721 cttttatgcc acattttatt tggggtcatt ccctgatatt acgggcacta tttattcaaa
    15781 actctgacga aaaacaggct gtcgtcagtt ttgacgtgac gaaacgaaat accgcgtgac
    15841 agccatcacg cggcagacat tttatttttt ctcgacgacg ggagccgcct gatacccgcc
    15901 gcccagcgat ttcatcaact gaatgctttg gatcacccgg cggctgtcca gcatcagtaa
    15961 tgacatctct tcggcaagta ctggcaaccg ggcttcagtg gcctgtaagc ggctggttaa
    16021 gccgcgctga taggcggcct cggcagcgcg ctgggtgaag cgcgttgctt ccacgcgttc
    16081 agcctgcatt tctcgctcgt cgttgagcgt ttgcagacgc gtgccgttga cggcaacgtc
    16141 acgcaccgcg ttcagtactg actggttgta acgttcaatc atcatgttgc tggcggcgcg
    16201 cgtgccttcg agattggcat tcaaccgtcc accgtcaaac agcggcaatt tcagacccgg
    16261 gatgaagttg aactggcgac tggttttttt gaataaggta tccaggtgga tggcatcaag
    16321 gccgaaaaac gctttgatat caaagctcgg gtagaacagc gcccgcgcgg aatccacctg
    16381 atctaatgac gcctgaacat accagcgcat ggcttgcaga tccgggcgtc tggcgagcaa
    16441 ctcataagag agtgtcgccg gaatgccggt ctggactcgt ggtaatgcca ccggtttgat
    16501 ctccggcata tcgctggccc ccgcgccaat caatgcacgc agagattctc gcgtttcggt
    16561 gatttgccct ttgacggcag caatttgttt atcgaccgcc agaatctgtg cccgcgcgcc
    16621 gtggaaaggc acttgtgctt ccagaccgtg cgccacttta ctctggtgcg ctttcaccgc
    16681 gtaatcaatc acatcgcgag tttgttctaa cagatcgagc atctgatagc tggcctgcat
    16741 actgtaataa agctgcgcta cgcccgtggt cagcgatagc tctactgctg cggtttctgc
    16801 cagcgcggca ttatgcgcgc caatggcggc ggcaaccgct gagcgatgca caccccacaa
    16861 atcaagatcc agtccggcaa acaaacctac tgtggcttcc gtatagtacg gcccgtccat
    16921 acccagtgcg ggcgcatcca tcgcataagg gcttaaaaag ccgttcgccg agacgcgttg
    16981 gcggttgagc atccctaacg ctgcgacctg taattgtgaa ccggcatcta acaaatcggc
    17041 ctgggactgc gctttttctt cccgcagttt cgcttcggcg agggtgtgtg aaccacttag
    17101 cgtccgttgg atcagcgcat ccagctgcgg gtcattgagt tgtttccacc actgcgcctg
    17161 cggccagccg gagctggcaa gatgaatatc gtcggccagt ttgatttgtt ccggtttgag
    17221 ctgttgatga ggtgcagaat ccttacgtac cagggcacag ccggaaatca gcgtcgtgct
    17281 gccgagaatg ctgcacaaca gcagacgtga aagttgacga ttgatcattg cgtggctcct
    17341 tgtgcctgtt ccgtggcggg cgttaatgcc ggggctgtcc agtctggcaa gcgggcgaaa
    17401 agctggcaga cgtgttgctc ctgttttaat aaggtgggca ggatggcctg cgaggcgggt
    17461 gtctcttcaa gcgttatttg aggagaacgg ctgagtgcgg ttgccagacc ggcagcgtat
    17521 ttctccaggg cgtcggcaaa ctgagctgct ctgtccggct gtagtgcgtt atccagcgcc
    17581 tgcgccgagt tccaggtggc atcccaggcg tgaagaatat cgcggccctg acgaataacc
    17641 gtttgcgaac gttcaatcag taaggcgcgt tcttcgctgt ccagttgacg ctccagcgcc
    17701 acgcgttggc acatctcttc acaggcatta aacgccgcat gcagaccgat acgaatttgc
    17761 agataagtgc gcagagccgt gacttcctgc tggcgtggga tccgcattac tttacttaac
    17821 atacccagcg tgccagccag tttttgcggc agtgtgcgcg cttcactttc aggccagaca
    17881 aaggtgtaaa tcaccgcgga caccacggta ccaatgatga tacccatggc gcgatcgcga
    17941 acttccacca ggtcgtacac cgggccaaaa acgttttcga gcgtggcgag cgcgaaggtg
    18001 accaccatct gtgtgccgat ataagaagag cgttcagagc tggtggcaat ccatgcgccc
    18061 aacaggaaaa tcggtgccag cacaaacagc aattcgacaa tattgtccag ccagggcatg
    18121 accagtagcg tgaataacag cgccagaatc gcgccgcaaa aggccccgcc aaaacgcagc
    18181 accatcttct ggtacgacga accgacattt gggttagcga cgatcacgca tgtcagcata
    18241 caggtgtgaa tgccttccca gtccacgccg ctgtagaagg tgtaacagat caaacaggcg
    18301 agcagtgttt ttaccgcgta gcgcatatag tctggattgg taaaagcatc ggcgaccatt
    18361 gatggcggtt tggcggcggg cgttggcggc gtattcgggt ccatctgacc cagttgtaac
    18421 aacgtctggc agatattctc caggttacat tcccgtgccg ccatcgcttc actttcagtg
    18481 atccgccagt cgctttgcca gcactgacct tcagcaacgg catgctgcag cttattgatt
    18541 tctgaggcta atttttgccg aaattcaata attgcctgag aatcagcaaa agaggtggga
    18601 tcgtagcgat tcagcgtcga gtaaatgtag gttaccgttg ccacgcagct ttgccaccat
    18661 gcgctttgag ttcgccagtt ggcatcgtcc gcgagacaaa agacattcag tttttgcagc
    18721 gccagcgcct ctctttcaat ccgcgtttcg ggtagcggtg cgaggctgtc cgtcaggtga
    18781 ctaatggcat catcaagccg atcattaagc gcttgatgca tttgcgtaat ggcacgactg
    18841 ggaaaccaca gcacgccgat taacgtcatc agcaaggttg gatagaggcc aacaacgata
    18901 caccacagcg ttaagcgcac gaccacttcc ggatagtcga gcatggcggg gaaggtttgc
    18961 ccgtaaatgg cgacaatggc gacagcgaaa aagaccagtc ccagccgatg ggtgcgcatc
    19021 aaaaacatac agcccatcag gatcggcccg gcgatgatca atcggatcaa cggttcgccg
    19081 tatgaccatt tgtagatcaa aaacaggctg ccgatctcca gcaccgtggc aaccacaaac
    19141 aagatcgcga caaatttggt gtaaaacgcg ttcgactgaa taccgtaaaa cagcactgcc
    19201 agcgataacg ccacaaaagg gatctcaaaa gtcatcgaga tcagaatcac cagcaggcag
    19261 cctacccaga gttgcacgat ctgcggcacg cggccgggtc gccgctcgct taactcttca
    19321 tgaaagaacg ccagcagcct gaccaccggt aatggcaggg agttgagcgc gctcattatt
    19381 gcggctcaag attagcgact gccgaagcgc cgatgcggaa catttccggg tcaggtttat
    19441 cgaccatgat tttgaccgga aaacgctggg caacgcggac ccagttaata gagcgagaaa
    19501 ctttcggcag gccgcccagc accaggccgc cgtcatccgg tagcacgccg tagccaatcg
    19561 aatccacttt accctcgaag gttttgccgc tgtcactcat caggcgaatc gttgcgggtg
    19621 tacctgagcg aatatttttc agatcggttt cgcggaagtt ggcgatcacg taccagtgac
    19681 gagtgtcgat tagggtaaaa atagggcgca tggcagaagc aaattgcccg acggaggttt
    19741 tgagggaaat gacccggcca tcaaacggcg cgcgaacggt cgccatttcc agatgaagtt
    19801 tggtcagggc aatatccgct tcgaccgccg cacgttgcgc caccagcgca tcgacgccgc
    19861 tgacggcgct ggcggctgac tgcgcctgta acaatacggc attaagatcg gcttctgcgg
    19921 cgcgctgcgc agttcttgca cggtcaacgt cttccgctga gacaaaaccc tctttcagta
    19981 atggctcggt gcggcgtaat gtatctgtgg cctgtttcgc ggcggcacgg gctttttcta
    20041 ccgtggcatt aatcgagtcg gcaccaaact gttgcgcatc aacgctacgc tgggtgagca
    20101 taatttgctt atccagcgcc gcgagggagg cttcagcttt cgccagattg gcttcgtacg
    20161 ggcgcgggtc aatgcggaac agcaaatcgc cctgtttgac tgcctggttg tcggtgaccg
    20221 ccagttctac aatgcggccg ctgacttccg gcaccacatc aatggtatct gctgacgcgt
    20281 aagcgtcatt agttgatggc gcactgtcta cgcgccagat aacgaaaaca agggcaacca
    20341 gcgccaatgc aaccactaac agagcaggga atttactgcg aggagctttt ttcggcgtac
    20401 tttccatgag cgcgtctcaa atagattaga aaaatgccag ccagaacagc atggcgtaga
    20461 gagcaaaaag ggcggtataa ataattccga caaatgccag attgatattt gcccgctgaa
    20521 taactcgcct cgtgatgagc atcaaaataa ggctggcaat ggcgcagaaa aaccagctgg
    20581 gataataagc accgatcacc gggattgccg gagataatga gcatcccgaa accatgaaga
    20641 cgggaattat ccaggctgtt tttttgagtt gcatcgccat gcgagagaga actgttggca
    20701 tattaaatac atcctgtctg ctgcgaacaa tatctgtagt gaatatcaat atccattgaa
    20761 ggcgtcagta tggctatttt ctcgatgaat aaaaatagct ttattcagcc a
//
LOCUS       DAZFYC010000082        20799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFYC010000082 DAZFYC010000000
VERSION     DAZFYC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.082"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            186..1568
                     /gene="dosC"
                     /locus_tag="ACHKW5_003378"
     CDS             186..1568
                     /gene="dosC"
                     /locus_tag="ACHKW5_003378"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO7512714.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1593..3992
                     /gene="dosP"
                     /locus_tag="ACHKW5_003379"
                     /gene_synonym="pdeO"
     CDS             1593..3992
                     /gene="dosP"
                     /locus_tag="ACHKW5_003379"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO7512715.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4250..4831
                     /gene="ddpX"
                     /locus_tag="ACHKW5_003380"
     CDS             4250..4831
                     /gene="ddpX"
                     /locus_tag="ACHKW5_003380"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO7512716.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            4845..6395
                     /locus_tag="ACHKW5_003381"
     CDS             4845..6395
                     /locus_tag="ACHKW5_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7512717.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVESDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6397..7419
                     /locus_tag="ACHKW5_003382"
     CDS             6397..7419
                     /locus_tag="ACHKW5_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7512718.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGYLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7416..8312
                     /gene="ddpC"
                     /locus_tag="ACHKW5_003383"
     CDS             7416..8312
                     /gene="ddpC"
                     /locus_tag="ACHKW5_003383"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO7512719.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8309..9295
                     /locus_tag="ACHKW5_003384"
     CDS             8309..9295
                     /locus_tag="ACHKW5_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7512720.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQLLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            9288..10214
                     /locus_tag="ACHKW5_003385"
     CDS             9288..10214
                     /locus_tag="ACHKW5_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO7512721.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(10270..10701)
                     /gene="osmC"
                     /locus_tag="ACHKW5_003386"
     CDS             complement(10270..10701)
                     /gene="osmC"
                     /locus_tag="ACHKW5_003386"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO7512722.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            11047..11262
                     /gene="bdm"
                     /locus_tag="ACHKW5_003387"
     CDS             11047..11262
                     /gene="bdm"
                     /locus_tag="ACHKW5_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO7512723.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            11364..11501
                     /gene="sra"
                     /locus_tag="ACHKW5_003388"
     CDS             11364..11501
                     /gene="sra"
                     /locus_tag="ACHKW5_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO7512724.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            11658..13355
                     /gene="maeA"
                     /locus_tag="ACHKW5_003389"
     CDS             11658..13355
                     /gene="maeA"
                     /locus_tag="ACHKW5_003389"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7512725.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRMEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            13489..14499
                     /gene="adhP"
                     /locus_tag="ACHKW5_003390"
     CDS             13489..14499
                     /gene="adhP"
                     /locus_tag="ACHKW5_003390"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO7512726.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            14646..14930
                     /locus_tag="ACHKW5_003391"
     CDS             14646..14930
                     /locus_tag="ACHKW5_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7512727.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(14981..15634)
                     /gene="fdnI"
                     /locus_tag="ACHKW5_003392"
     CDS             complement(14981..15634)
                     /gene="fdnI"
                     /locus_tag="ACHKW5_003392"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO7512728.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIIEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIILRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(15627..16511)
                     /gene="fdnH"
                     /locus_tag="ACHKW5_003393"
     CDS             complement(15627..16511)
                     /gene="fdnH"
                     /locus_tag="ACHKW5_003393"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO7512729.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(16524..19571)
                     /gene="fdnG"
                     /locus_tag="ACHKW5_003394"
     CDS             complement(16524..19571)
                     /gene="fdnG"
                     /locus_tag="ACHKW5_003394"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(18984..18986),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7512730.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLLWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKLLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            19803..20684
                     /gene="yddG"
                     /locus_tag="ACHKW5_003395"
     CDS             19803..20684
                     /gene="yddG"
                     /locus_tag="ACHKW5_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO7512731.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5087 a         5309 c         5285 g         5118 t
ORIGIN      
        1 aaaaataatt acgatgggct atactcttct gaagaacagt agcgtagatt gaaactgtcc
       61 taagtcgctt atttcttttc agtatatctt catatttcag gagaatatgg tgcagttagt
      121 cgatctcgca cgttgcgttt cgttttgatt ccgtctgatt tcccagcctt ataagggtgt
      181 gaattatgga gatgtatttt aaaagaatga aagatgagtg gaccggactt gtcgaacagg
      241 cagatcctct cattcgtgct aaagccgcag aaattgccct tgcgcatgct cattatctga
      301 gtatcgagtt ttatcgaatt gtccgcatcg acccgcatgc cgaagaattc ttgagtaatg
      361 aacaagttga gcggcagttg aaaagtgcga tggaacgctg gattattaac gtgctttctg
      421 cccaggttga cgatgtcgaa aggctaatac aaatccagca taccgtcgcg gaagtgcatg
      481 cccgcatagg aattccggta gaaattgtcg agatggggtt tcgggtgctg aaaaaaatcc
      541 tctatccggt aatcttctct tcggattatt ctgccgcaga aaaacttcag gtctaccatt
      601 tctcgattaa cagtattgat atcgctatgg aagtgatgac ccgcgcgttt acctttagtg
      661 acagtagtgc ctcaaaggaa gatgaaaact atcgtatctt ctcgttactg gaaaacgccg
      721 aagaagaaaa agaacggcaa atagcctcaa tactttcatg ggaaatagat attatctata
      781 aaatcctgct ggattctgat ttaggcagta gtttgccttt aagccaggct gattttggcc
      841 tgtggtttaa ccataaaggt cgacattatt ttagcggcat tgccgaagta ggccatatct
      901 cccgtctgat tcaggatttc gacggtattt tcaatcaaac catgcgtaac accaggaatt
      961 tgaataacag aagtttgcgg gtgaagtttc tattacagat aagaaatacc gtatcgcaaa
     1021 ttattacctt gctgcgtgaa ttgtttgaag aagtatcgcg ccacgaagtc ggtatggatg
     1081 tactgacgaa attacttaac cgccgtttcc tgccgactat cttcaaacgc gaaattgccc
     1141 atgccaaccg gaccggtaca ccgctgtcag tgctgattat tgacgttgat aaattcaaag
     1201 agatcaacga tacgtggggc cataacactg gcgatgaaat tctgcgtaaa gtctctcagg
     1261 ccttttatga caacgtccgc agtagtgatt atgttttccg ctacgggggc gatgaattta
     1321 tcattgtttt gactgaagct tctgaaaacg aaacgttacg taccgcagaa cgtattcgca
     1381 gtcgggtgga gaaaaccaaa ctgaaagccg caaacggcga ggatattgcc ctctcacttt
     1441 ccatcggtgc cgccatgttt aatggtcatc ctgactatga gcgcctcatt caaatagccg
     1501 atgaagctct gtatatcgcc aaaagacgag gtagaaaccg tgttgaactc tggaaagcca
     1561 gtctttagat gcgccaggat gcagaggtaa tcatgaagct aaccgatgcg gataatgccg
     1621 ccgatggcat ttttttcccc gcccttgagc aaaatatgat gggtgcggtg ttaattaacg
     1681 aaaatgatga agtgatgttt ttcaaccccg ccgcagagaa gctctggggg tacaaacgtg
     1741 aagaagtcat tggcaataac attgatatgc tgattccgcg ggatttgcgt cctgcgcatc
     1801 ctgaatacat tcgtcataac cgtgaaggcg gtaaagcgcg tgtcgagggg atgagtcggg
     1861 agctgcagct ggagaaaaaa gacggcagta aaatctggac ccgttttgcg ctatcgaaag
     1921 tgagcgccga ggggaaagtt tattacctgg cgctggtacg ggatgccagc gtagaaatgg
     1981 cgcaaaaaga acagactcga caattgatta ttgccgttga ccatctcgac cgaccggtga
     2041 ttgtcctcga tccggaacgc catattgtgc agtgcaatcg cgcatttacc gaaatgtttg
     2101 gttactgcat tagcgaagcc agcggtatgc agcccgatac actcctgaac attcctgaat
     2161 tccctgccga taaccgcatt cgtttacaac agttgctatg gaaaaccgcc cgcgatcagg
     2221 atgaatttct gctgttgacg cgcaccggtg aaaaaatctg gattaaagcc tctatcagcc
     2281 cggtttatga cgtgctcgcg catctgcaga acctggtaat gaccttctcg gatatcaccg
     2341 aagaacggca aatccgccag cttgaaggca atattctcgc cgccatgtgc agcagcccgc
     2401 catttcatga aatgggggaa atcatttgtc gtaacatcga atctgttctc aacgaatcgc
     2461 atgtttcgct gttcgcactg cgcaacggga tgccgataca ctgggcgtca tcttcgcacg
     2521 gtgcagaagt tcaaaatgcg caaagctggt cagcgaccat tcgtcagcgt gatggcgcgc
     2581 ctgcggggat cctgcaaatt aaaacctcgt caggagcaga aaccagcgcc tttatcgaac
     2641 gcgtggcaga tatcagtcag catatggccg cgctggcgct ggaacaggaa aaaagccgcc
     2701 agcatattga acaactcatc caatttgatc cgatgaccgg tctgccaaat cgcaataacc
     2761 tgcacaatta cctcgatgac ctggtcgaca aagccgtctc tccggtggtg tatctcatcg
     2821 gtgttgacca tattcaggat gtgattgata gccttggcta tgcgtgggcc gatcaggcat
     2881 tgctggaagt ggtcaatcgc tttcgtgaaa aactcaaacc ggatcagtat ctctgtcgta
     2941 tcgaaggtac gcagtttgtc ctcgtgagcc tggaaaacga cgtcagtaac attacccaaa
     3001 tcgccgatga gctacggaat gtggtcagca agccgataat gattgacgat aaacccttcc
     3061 cgcttacctt gagtattggc atcagctacg acgtgggtaa aaaccgcgat tacctgctct
     3121 ccactgctca caatgcaatg gattatattc gcaagaatgg cggtaacggc tggcagttct
     3181 tcagcccggc gatgaacgaa atggtaaaag agcgtttggt tttaggcgca gcgctgaaag
     3241 aagcgattag caataaccaa ctgaaacttg tttaccagcc gcaaatcttc gcagaaacgg
     3301 gtgaactgta cggcatcgaa gcccttgcgc gctggcacga tccccagcat ggtcatgtgc
     3361 ccccttcacg gtttattcct ctcgcagaag agattggtga aatcgaaaat attgggcgct
     3421 gggtcatcgc ggaagcttgc cgtcagttag cagaatggcg tagccagaat attcatatcc
     3481 cggcgctttc cgttaacttg tcggcactgc actttcgcag taatcaactg cctaatcagg
     3541 tgtctgatgc aatgcacgcc tggggtattg acggccacca gctgacggta gaaatcacgg
     3601 aaagcatgat gatggaacac gataccgaaa tctttaagcg cattcagatc ctgcgtgata
     3661 tgggcgtggg cttatcggta gatgattttg gtacgggctt ttccggatta tcccgcttag
     3721 tcagtcttcc ggtaacggaa atcaaaattg acaaaagttt tgtcgatcgt tgtctgaccg
     3781 aaaaacgcat ccttgcctta cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa
     3841 ccgtcgtggc ggaaggggtc gaaaccaaag agcaatttga gatgctacgc aagatccact
     3901 gtcgcgttat tcagggatat ttcttttccc gccccctacc cgccgaagaa attccaggct
     3961 ggatgagcag cgtgttaccg ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt
     4021 tttcatttaa cgtgacactg tcacttaaat actgtgattt cagctacgat tccgggagat
     4081 tccttcctta acttcccctc ttgcaccaaa actgcactac gctgcacaaa tgttgatcaa
     4141 aagttaaaaa atataacttt aagccagcat tctggcgctt atcccggcat ggcatgagat
     4201 ctgcataagc ggaaagcgca gcaatttttg tcttatacag gaaccgttta tgtcggatac
     4261 caccgaactg gttgatttag ccgtaatctt ccctgatctg gagatcgaat tgaaatacgc
     4321 ctgcgctgat aacatcacag gtaaagctat ttatcagcaa gcgcgttgtc tgctacacaa
     4381 ggatgcgatt accgcgctgg cgaaaagtat cagtatcgcc cagctgtcag gcttacaact
     4441 ggtgatttac gatgcgtatc gcccacaaca agcacaggcg atgttgtggc aagcctgccc
     4501 agacccgcaa tatgttgttg atgtgacggt cggttctaat cacagccgtg gcacggcgat
     4561 cgacctgacg cttcgtgatg agcacgggaa catcctcgat atgggcgcag ggttcgatga
     4621 aatgcacgag cgctcccatg cctatcaccc ttccgtcccg cccgccgctc agcgcaatcg
     4681 gctgttgctg aatgcgataa tgactggtgg cggttttgtc ggtatctcca gcgaatggtg
     4741 gcacttcgaa ttaccccagg cagcgagtta ccctctgctc gctgatcaat tcacctgttt
     4801 tatatcccct ggcacacaac acgtcagtta atcaggaaac tgtcatgaag agatcgatac
     4861 cgtttcgtcc cacattgctc gcgctcgtcc ttgccacaaa tttcccggtt gcgcacgccg
     4921 ccgtaccgaa agatatgctg gtgattggta aggccgccga tccacaaacc ctcgacccgg
     4981 cggtaacaat agataataac gactggacgg tgacctaccc gtcttatcag cggctggttc
     5041 agtacaaaac ggacggtgat aaaggctcaa ccgacgttga aagcgatctg gcaagtagct
     5101 ggaaagcgtc tgacgatcaa aaagagtgga cgttcaccct gaaggacaat gctaaatttg
     5161 ccgatggcac accggtcact gccgaagcag taaaactctc ttttgagcgg ctactaaaaa
     5221 tcggccaagg gccagcagaa gcatttccca aagatttaaa gattgatgct cccgacgaac
     5281 atacggtgaa gtttaccctt agccagccat tcgcaccgtt cctctacacg ctggcgaatg
     5341 acggtgcatc cattatcaat ccggcggtct taaaggaaca tgcagcggat gatgctcgcg
     5401 gcttcctcgc gcaaaatacc gccggttccg gaccatttat gctgaaaagc tggcaaaaag
     5461 gtcagcaatt agttctggtg ccaaatccgc attaccccgg caataaaccg aactttaagc
     5521 gagtatcggt aaaaattatc ggtgaaagtg cctcccgtcg cctgcagctc tcccgtggcg
     5581 acattgacat tgccgatgcg ctgccggtgg atcaactcaa cgccctgaag caggaaaata
     5641 aagtcaatgt ggcagagtat ccgtcactgc gcgttaccta tctgtatctg aataacagca
     5701 aagcgcctct taatcaggcg gatctgcgtc gggccatttc ctggtctacc gattatcagg
     5761 gcatggttaa cggcattctg agtggtaacg gaaaacagat gcgcggcccg attccggaag
     5821 gcatgtgggg ctacgatgcg acggcaatgc aatacaacca tgacgaaacg aaagccaaag
     5881 ccgaatggga taaagtgacg agcaaaccca ccagcctgac gtttctctac tccgataacg
     5941 atccgaactg ggagcctatt gctttggcga cacaatccag tctcaacaag ctgggcatca
     6001 atgtgaagct ggaaaagctg gcgaacgcca ccatgcgcga cagagtgggt aaaggtgatt
     6061 acgacattgc gattggcaac tggagtccgg attttgccga cccgtatatg tttatgaatt
     6121 actggtttga gtcagacaaa aaaggtctgc cgggtaaccg ctcgttctat gaaaacagtg
     6181 aggtcgataa gttactgcgc aatgcgcttg cgaccaccga ccagacgcag cgtacccggg
     6241 actaccagca ggcacagaaa atcgtcattg atgacgctgc ttatgtgtac ctgttccaga
     6301 aaaactacca actggcgatg aacaaagagg tgaaaggctt tgtgttcaat cccatgctgg
     6361 aacaggtctt caatatcaat accatgagta aataacgtga ccttctggag tattttacgc
     6421 caacgctgct gggggctggt gctcgtggtg gcgggcgtct gcgtgattac gtttattatc
     6481 tcgcatctga tccctggcga tccggcgcgg ttactggcgg gtgaccgcgc cagcgatgct
     6541 atcgtggaaa atattcgcca gcaactggga ctggaccagc cactgtatgt acagttttac
     6601 cgctacgtca gcgacctgtt tcatggttac ctgggaacat ccattcgtac cgggcgtccg
     6661 gtgctggaag agttgcgtat atttttcccg gcgacgctgg agctggcttt tggtgccctg
     6721 ctgctggcac tcctgattgg catcccgctg ggcatactct ctgcagtctg gcgaaatcgc
     6781 tggctggatc atctggtgcg aataatggcc attaccggaa tctccacacc tgcgttctgg
     6841 cttgggctgg gcgtcattgt gctgttttat ggtcatctgc aaattcttcc cggcggcgga
     6901 aggcttgatg actggctgga tccaccaacg cacgttaccg gcttttatct gctcgatgcg
     6961 ctgcttgaag gcaacggtga agtcttcttc aatgcgttgc aacatctcat cttaccggca
     7021 ttaacgctgg cgttcgttca cctgggaatt gtcgctcgcc agatccgctc agcgatgctg
     7081 gaacaattga gtgaagacta cattcgtacc gcccgggcca gcggcttgcc cggctggtat
     7141 atcgttttat gttatgcgct acccaatgcg ttgatcccat cgattaccgt attgggtttg
     7201 gcgctgggcg atttgttgta tggcgcagtg ctcaccgaaa ccgtttttgc ctggcccgga
     7261 atgggtgcat gggtagtaac atcaatacag gcgctcgact tcccggcagt gatgggcttt
     7321 gccgtcgtgg tttcatttgc ttatgtgctg gtcaacctgg tggtggattt gctctatttg
     7381 tggattgatc cgcgtatcgg acgtggaggt ggtgaatgat gctaagcgag gaaacgtccg
     7441 ccgtacgccc acaaaaacaa acgcgattta acggtgcaaa actggtatgg atgctgaaag
     7501 gcagtccgct caccgtgacc ggcgcagtca tcattgtatt aatgctattg atgatgattt
     7561 tttcaccgtg gctggcgacg catgatccca acgccattga tttaaccgcc cgccttttgc
     7621 cgccttctgc ggcgcactgg tttggcaccg atgaagtggg acgcgatctg ttcagccgcg
     7681 tactggtcgg cagtcagcaa tcaattctcg ccggattagt cgtggttgcc attgcgggta
     7741 tgattggttc gctactcgga tgtctatccg gtgtgcttgg cggacgcgca gacgccatca
     7801 tcatgcgcat catggacatt atgctgtcga ttccttcgtt ggtactgaca atggcactgg
     7861 ctgccgctct cgggccgagt ttatttaacg ccatgctggc gattgctatt gtgcgaattc
     7921 ccttttatgt gcgcctggcg cgggggcaag cattagttgt acgccagtat acctatgttc
     7981 aggcggcgaa aacctttggt gcgtcgcgtt ggcatctgat cagctggcat attttacgta
     8041 actccctgcc gccgctgatc gtgcaggcat cgctggatat cggtagcgcg attttaatgg
     8101 ccgccacgtt gggatttatt ggcctgggtg ctcaacaacc gagtgctgaa tggggcgcga
     8161 tggtagcgaa tggtcgcaac tatgtactcg atcagtggtg gtattgcgca tttccggggg
     8221 cagcgatttt gcttaccgcc gttgggttta atctctttgg tgatggtatt cgcgatctgc
     8281 ttgacccgaa agcaggagga aagcagtcat gacccaaccc gttctggaca ttcaacagct
     8341 gcatttgagt ttccccggtt ttaacggtga tgttcacgcg ctcaacaatg tgtccttgca
     8401 gattaaccgc ggcgaaattg tcggtctggt gggagaatcc ggctcaggta aatcagtcac
     8461 cgcaatgctg attatgcgtc tgctacagac gggcagttat tgcgtacatc gggggcagat
     8521 ttcactgctg ggagacgatg ttcttaacgc tcgggaaaaa cagcttcgtc agtggcgcgg
     8581 cgcacgagtg gcgatgatct ttcaggaacc gatgaccgcc ctcaatccga cacgtcgaat
     8641 aggtcttcag atgatggacg tgatccgcca tcatcaacca ataagtcgtc gggaagccag
     8701 agctaaagcg attgccctgc tggaagagat gcaaatcccg gatgccgtgg aagttatgtc
     8761 gcgctatccg tttgagcttt caggtggtat gcgccagcgg gtaatgattg cgctggcatt
     8821 ctcctgcgag ccgcaattga ttattgccga cgaaccgact acggcgctgg acgtcacgct
     8881 acagttgcag ctactgcgtt tgcttaaaca taaagcccgc gccagtggaa ctgcggtact
     8941 gttcatcagc catgatatgg tcgtggtgtc gcaactgtgc gatagcgttt acgtgatgta
     9001 tgccggaagc gtaattgaaa gcggcgtgac ggcagacatt atccatcatc cccggcatcc
     9061 gtataccatt ggtttgctgc aatgcgcacc agaacatgga gtaccacgcc agccattacc
     9121 cgccattcca gggacggtac caaacctcac ccatttgcct gaaggctgcg cttttcgcga
     9181 tcgttgctat gcggcaggtg cacagtgtga aaacgtcccg gcgctgacag cgtgtggtga
     9241 caacaaccag cgctgcgcct gttggtatcc gcagcaggag gtcataagtg tctgacacgt
     9301 tattaacgtt acgcgacgtc catatcaatt tcccggcccg taaaaactgg cttggtaaaa
     9361 ctacggaaca tgttcatgcc attaatggta ttgatttaca gatccgccgt ggtgaaacct
     9421 tagggatcgt cggcgagtca ggctgcggca aaagcaccct cgcacagctt ttaatgggca
     9481 tgctgcaacc gagccacggg cagtacatcc gttcaggctc acaacgcatt atgcagatgg
     9541 tgtttcagga cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc atcatcacag
     9601 aaccgctctg gatagctaag cgtagtagtg aacaacagcg gcgagcgttg gcagaggagc
     9661 tggctgtgca ggtgggtatt cgtccggagt atctcgaccg cctgcctcat gcgttctccg
     9721 gcgggcagcg gcaacgtatc gccattgcca gagcactctc ttcgcagcct gacgtgattg
     9781 tgcttgatga gccaacctct gcgctggata tctccgtgca ggcgcagatc ctcaatttac
     9841 tggtaacgct acaggaaaat cacgggctga cctatgtgct gatttcacac aatgtctcgg
     9901 tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg
     9961 gcgacgcgca acaggtgcta acggctccgg cacatccata cacccgatta ttgctggatt
    10021 ccctccccgc cattgataaa ccgctggagg aagaatgggc attacgtaaa acggatctgc
    10081 cgggaaaccg cacgttgccg caaggctgtt ttttctacga acgttgcccc cttgcaacca
    10141 gcggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgag ctccggtgct
    10201 ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc
    10261 ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga
    10321 aaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc
    10381 cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt
    10441 atccagcgac acatcggcgg tggtatcaat cgatgttggc gtaaatcccg cttcccccag
    10501 cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg
    10561 gttagttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac
    10621 accactctcg gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg
    10681 acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact
    10741 acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaaatccgg cagaaatatt
    10801 acttctgcac ttaaattaaa tttccacaat agataaccgg gaatatataa cccaagagat
    10861 attgcattaa tattaatcaa ctcaccacaa taataatacc tatcaatatc atgaggttaa
    10921 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    10981 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    11041 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    11101 gccattgagc aaggccttcg ggcagagcac ggtgttgtca ctgaagatga cattctcatg
    11161 gagctgacca aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    11221 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    11281 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    11341 agcaacagga ttgaggagtt aaaatgaaat caaaccgtca ggcacgtcat attcttggac
    11401 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    11461 taaataatcc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    11521 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    11581 tggggccgac gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc
    11641 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    11701 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    11761 aagaacgccg taacttcaat ctgctggggt tactgccgga agtggtcgaa accatcgaag
    11821 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    11881 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    11941 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    12001 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
    12061 tggacgatat tctgcaaaac gtaccaaacc ataatatcaa agtgattgtg gtgactgacg
    12121 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    12181 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    12241 tggatgtcgg aacgaacaac caacagttgc ttaacgatcc gctgtatatg ggctggcgta
    12301 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    12361 aacaacgctg gccggacgtg ctgttgcagt ttgaagactt cgcacaaaaa aatgcgatgc
    12421 cgttacttaa ccgctatcgc aatgaaattt gttcctttaa cgatgacatt cagggcactg
    12481 cggcggtaac agtcggcacc ctgatcgcag caagccgcgc ggcaggtggt cagttaagcg
    12541 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    12601 tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    12661 tcgatcgctt tggcttgctg accgacaaga tgccgaacct gctgcctttc cagaccaaac
    12721 tggtgcagaa gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    12781 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    12841 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    12901 cgctgtccaa cccgacgtca cgcatggaag ccacaccgca ggacattatc gcctggactg
    12961 aaggtaacgc gctggtcgcc actggcagtc cgtttaatcc agtggtatgg aaagataaaa
    13021 tctaccctat cgcccagtgt aacaacgcct ttattttccc aggcatcggg ctgggtgtta
    13081 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    13141 ctcagtattc gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata
    13201 ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    13261 tggcggtgaa aacctctgcc gaagccctgc aacaggccat tgacgataat ttctggcaag
    13321 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    13381 cgggctattt ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    13441 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    13501 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
    13561 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    13621 aatggcgatt ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg
    13681 gcagaagttg gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    13741 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    13801 tcagttaaaa atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc
    13861 gccgattacg cggtaaaagt gccagatggc ctggactcgg cggcggccag cagcattacc
    13921 tgtgcggggg tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg
    13981 attgctatct acggtcttgg cggtctgggt aacctcgccc tgcaatacgc gaagaatgtc
    14041 tttaacgcga aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa
    14101 atgggtgcag atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag
    14161 aaaaccggtg gcgctcacgc tgcggtggta acagcagttg ctaaagctgc gtttaactcg
    14221 gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccagagtct
    14281 atgagcctgg atatcccacg ccttgtgctg gatggcattg aagtggtcgg ttcgctggtc
    14341 ggcacgcgcc aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg
    14401 aaagtcgccc tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc
    14461 aaaatccgtg gccgtatggt gattgatttc cgccgttaag aggcctttgc tgcgagtgcc
    14521 agtcctggtg tcgcagcatc gcactctctc cgacattaaa tggctaaatg gattattgtt
    14581 ccttcctacg caacaataat gatagtgttc ctgatgaagg aacactactg agagtaaaga
    14641 cactgatgaa aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg
    14701 aacttaatgt cagcctgcgc gagtttgcca gagcaatgga aattgctccc tcaacggcaa
    14761 gccgattgct gaccggaaaa gcagctttga caccagaaat ggcgattaaa ctttccgtgg
    14821 tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg
    14881 cagaaaaaac ggtggatgtg tcgcgactac gccgtctggt tacgcaataa aaaaatggcg
    14941 cttcttaaag cgccattttc agcttaaagt tcaaaagaga ttatatccct tcttcactct
    15001 cttttttcgc ttctgccttc tcgatttcac gataccagcg cggatggtgt ttcttcgccc
    15061 agcgacggct caccttccct tcgatcatcc ctttaatcga ccctttcacc cagaatgcca
    15121 tatacatatg gatcaggatg gcgtggatca ggatgatacc cgcagccgcg tggatcagca
    15181 ggctgtagcg aacaacctgc atcgggaagt actgcgcaaa gtacggacgc aagataatca
    15241 ccccggtcac cagcagcacg aaaatcatgc tcatgatcga ccagaacatc atcttttgcc
    15301 cggcgttgta cttaccgaca tccgccactt tatgctcatt gcctttcaat acttcgataa
    15361 tgttcaacag ccacggaata tctttcttat ccgggatgtt gtgatgcaca aaacgcacaa
    15421 acataaacat cagtgcgacg aaaatcgcaa tgccgaaaaa cgggtgcaaa atgcgtccca
    15481 tctgcggcgt accgaaggtt tgcgtcagcc attgcagcgt cgggaagaag aacgaaatcc
    15541 cggacagcgc caccaggaag aagcaaatca ccacggtcca gtgacaggcg cgatcaataa
    15601 atttggtgcg cacaatcatt ttcgacttac tcatgatgat cctcctcgtc atcgtccact
    15661 tccttattcg ggccaatacc gatgtagtgg aaaatcaacc cggcaaaagt ggcaataaag
    15721 ccagccgctg ccagcggttt caacgcgcct ttccacaggc ttaccgaggt gtcgatcttc
    15781 ggatctttcg gcagaccgtg atacagctcc ggctgatcgg cgtgatgcag cacgtacata
    15841 acgtgcgtac caccgacccc ttccgggttg tagacgccag catgttcgta accacgcgct
    15901 ttcagtttcg ccacgcgctg ttccgccagc tccagcatct ccttcttggt gccgaagtgg
    15961 atagccccgg tcggacaggt tttcacacaa gccggttcct ggccgacgct gacgcgatcg
    16021 acgcagagcg tgcatttata tacccggtta tcctctttgt tgaggcgcgg aatattaaac
    16081 ggacacccgg caatgcagta accacagccg atgcagtttt ccgactggaa atcgacaatc
    16141 ccgttagcgt actgaatgat tgcaccagca gacgggcacg ccttcaggca gccgggatct
    16201 tcacagtgca tacagccgtc tttacggatc agccactcca gcttgccgtt ctgttcggtt
    16261 tcgctaaagc gcatcaccgt ccaggacttg gcgctcagat cggcggggtt atcgtaaacc
    16321 ccgacgcagt gccccacttc atcacggatg tcgttccact ccgaacacgc cacctgacag
    16381 gctttacagc cgatacaggt ggaaacgtcg ataagttttg cgacttctgc tttgtaatca
    16441 cgcacctgag aaggcggcgt gatggagtta gttgcggacc ttttgataat gtcctgcgtt
    16501 tccatagcca tctgttcgcc cccttacgcc ttctcgatgt tgactaagaa cgctttatat
    16561 tccggcgttt gcgagtttgc atcaccgaca ttcggcgtca gagtgttagc gatataacct
    16621 ttacgcgcga caccctcaaa gccccagtgg attggaatac ccaccgtttc aacctgctga
    16681 ccatttacat tcagtagttt cagacgacgc gttaccacag ccaccgcgcg gataaagcca
    16741 cgcttgcttg agacagtgac acgatcgcca ttattaatgc ctttcgccgc cgccagcgtt
    16801 tcgctgattt ccacaaactg ttccggctga gcaattgcgt tgagcaatgc gtgcttggtc
    16861 caggtgtgga agtgctcggt cagacgatag gtcgtaccca catacgggaa ctgctctttt
    16921 ttacccatcc gcagtgcgtc ttgttcatac agacgaacaa ccgggttaga caccacgttc
    16981 ggatgcagcg ggttagtgcc cagcggcgtt tcaatcggct cgtagtgttc cgggaacgga
    17041 ccttccgcca ttttgttgat ggcaaacagg cgtcccatcc cttccggctg catgataaac
    17101 ggcccggttg gcgtacccgg tgcggcattg ccgaagtcag gaatatcgtt acccgtccac
    17161 ttgctgccgt tccactggat cagcatccgt ttcggatccc acggtttacc gttgatatcc
    17221 gccgaagcac ggttgtacag cacgcgacgg ttgagcggcc acgcccaggc ccatcccagc
    17281 gtattcccca gaccggacgg gtcggagtta tcgcggttag ccatctggtt gccctgctct
    17341 gtccagctac cggtgtagat ccagcaagaa gatgcggttg taccgtcatc acgcagatgc
    17401 gcaaagctac tcagcaactg acctttcttc gcaatcagca cgccattagc atcatagaga
    17461 tcttccagcg catagccgtt gttctcttta gctacttcgt cagattgcgg ttcgtgcggc
    17521 tgcttgtagt tccagctcat cttcatcagc ggttctacgc ctttaccacc ttcggcctgg
    17581 tacagctcgc gcaggtgatg gtagataccc gccagaattt caccgtcgtt acgcgcttcg
    17641 cccggcgcgt cctgaccttt ccagtgccac agcagccagc gaccggagtt agcaatagaa
    17701 ccatcttctt cagcaaagca ggtcgaaggc agacggaata cttcagtctg aatagacgcc
    17761 ggatcgacat cgttcgactc gccgtggttc tgccagaagg ttgaggtttc agtcaccagc
    17821 ggatcgataa ccaccatgta cttcagcttg cttaggcagc tcaccacttt gtttttgtcc
    17881 gggaaggacg caaccgggtt aaagccctgg cagaaataac cggtgacttt gccttcatcc
    17941 atcatgttga aatacttgat gacgtcgtag gtctggtccc acttcggcag ccagtcatag
    18001 ccccagttgt tctctttctg cgcggcatcg ccatagaaag atttcatcag gctaacgaag
    18061 aacttcggat agttgctcca gtagttcacc tgaccagcca gcgtcgcttt cggcgtgttc
    18121 gcttccagat acgactgcaa atcaacctgt ttttctgacg gcagcgtcag ataacctggc
    18181 aggctggtag agagcagacc taagtcagtc agaccctgaa tgttggagtg accacgcaat
    18241 gcattcacgc cgccaccggc catacccatg ttaccgagca gcaactggat catcgccata
    18301 gtacggatgt tctgcgcacc cacggtgtgc tgcgtccagc ccagcgcgta caggaaggtg
    18361 gttgtgcgat ccggtgcgct ggtggaggcc agcacttcac acactttcag gaagtcggct
    18421 tttggcgtac cgcagatgtt ttcaacgacg tctggcgtgt agcgggaaac gtgctctttc
    18481 agcaggttcc acacacagcg cggatgagtc agtgtttcat cgcgtttcgc atagccgttt
    18541 tcatcgaact gatagttcca ggacgatttg tcgtactggc gtttttcagc gtcataaccg
    18601 ctgaacaggc catcttcgaa agcaaaatca tcacgcacca gcaggctggc gttggtgtaa
    18661 tgtttaacgt attcggcgtt gattttgttg ttttcgatca ggtagcgcaa aacgccagac
    18721 aggaacgtaa tgtccgtacc ggaacgaata ggtgcgtaaa tatccgccac agaagcggta
    18781 cgcgtaaaac gaggatcgac aacgatcaag gttgcatcgt tgttgttttt cgcttccatc
    18841 gcccagcgga aaccgacggg atgcgcttca gcagcgttac cgcccattac cattacgacg
    18901 ttagcgtttt tgatatccac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca
    18961 agacttgcta ccgttggtcc gtgtcagacg cgcgcctggt tgtctaccgc cagcatcccg
    19021 agggagcggg caaatttctg tgtcagcatc ccggtttcgt tgctggcacc ggaggcacac
    19081 agcataccgg tagaaagcca acggtttacc gttacgccct gctcgttctt ttcaataaag
    19141 ttagcgtcac ggtcagcttt catcagcttt gcaatacggg agaatgcttc ttcccagcta
    19201 atgcgctgcc atttgtcaga acctggcgca cgatattccg ggtagcgcag acggttttcg
    19261 ctgttgacgt aatccagcaa accggcccct tttgggcaca gcgcaccacg gcttaccgga
    19321 tgatccgggt caccttcaat gtgataaatc gcttctctgg cgtttttcgc gccatcaccc
    19381 aggctataca tcaatagccc gcaacctacg gaacagtatg tgcaggtgtt acggatctct
    19441 ttagcgcgta ataatttgta gtttcgcgcc tgagccagtg cttgcttcgg ggcaaagccc
    19501 aatgccgcta ccgttgttcc agccataccg cccgcgcaga ttttaaaaaa ttgtctgcga
    19561 ctgacgtcca ttgctttcct cttttttcag gggtattact gcgcgaggaa actaacagta
    19621 aaattctgaa tctttttgcg cgaaatcaaa aagaacacgt gtttgccaca aaataatccg
    19681 cctgcgcaaa ttgacctacc tcaatagcgg tagaaaaacg caccactgcc tgacaggcca
    19741 gttaaaaaaa tgctataaaa ttcagcttaa tttttaacgg caagagagac aaaacagcga
    19801 gcatgacacg acaaaaagca acgctcatag ggctaatagc gatcgtcctg tggagcacga
    19861 tggtaggatt gattcgcggt gtcagcgagg ggctcggccc ggtcggcggc gcagctgcta
    19921 tctattcatt aagcgggctg ctgttaatct tcacggttgg atttccgcgt attcggcaga
    19981 ttccgaaagg ctatttactc gccgggagtc tgttattcgt cagctatgaa atctgtctgg
    20041 cgctttcctt agggtatgcg gcgacccgtc atcaggcgat tgaagtgggt atggtgaact
    20101 atctatggcc cagcctgaca attctctttg ccattctgtt taatggtcag aaaaccaact
    20161 ggctgattgt accaggatta ttattagccc tggtcggcgt ctgctgggtg ttaggcggtg
    20221 ataatgggtt acattatgat gaaatcatca ataatattac caccagccca ttgagttatt
    20281 tcctggcgtt tattggtgcg tttatctggg cagcctattg cacagtaacg aataaatacg
    20341 cacgcggatt taacggaatt accgtttttg tcctgctaac gggagcaagt ctgtgggttt
    20401 actattttct cacgccacaa ccagaaatgg tatttagcac gcccgtcatg attaaactca
    20461 tctctgcggc atttacctta ggatttgctt atgctgcatg gaatgtcggt atactgcatg
    20521 gcaatgtcac cataatggcg gtaggctcgt attttacgcc cgtactttcc tcagcgcttg
    20581 cagccgtgct gctcagcgcc ccgctgtcgt tctcattctg gcaaggcgcg ctaatggtct
    20641 gcggcggttc cctgctctgc tggctggcga cacgtcgtgg ttaaaataat tgccgggtct
    20701 acggcccggc agttaatcag ataaatcccg acaatattaa aaaataaatt tcaaatacca
    20761 gtaattcacc ttatatctca tttcgacttc gatcacata
//
LOCUS       DAZFYC010000083        20770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFYC010000083 DAZFYC010000000
VERSION     DAZFYC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.083"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..930
                     /gene="exuR"
                     /locus_tag="ACHKW5_003396"
     CDS             154..930
                     /gene="exuR"
                     /locus_tag="ACHKW5_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO7512732.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1170..1253
                     /gene="yqiM"
                     /locus_tag="ACHKW5_003397"
     CDS             1170..1253
                     /gene="yqiM"
                     /locus_tag="ACHKW5_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO7512733.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1275..1937
                     /gene="yqjA"
                     /locus_tag="ACHKW5_003398"
     CDS             1275..1937
                     /gene="yqjA"
                     /locus_tag="ACHKW5_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO7512734.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1941..2324
                     /gene="mzrA"
                     /locus_tag="ACHKW5_003399"
     CDS             1941..2324
                     /gene="mzrA"
                     /locus_tag="ACHKW5_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO7512735.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2471..2839
                     /gene="yqjC"
                     /locus_tag="ACHKW5_003400"
     CDS             2471..2839
                     /gene="yqjC"
                     /locus_tag="ACHKW5_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO7512736.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2877..3182
                     /gene="yqjD"
                     /locus_tag="ACHKW5_003401"
     CDS             2877..3182
                     /gene="yqjD"
                     /locus_tag="ACHKW5_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO7512737.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3185..3589
                     /gene="yqjE"
                     /locus_tag="ACHKW5_003402"
     CDS             3185..3589
                     /gene="yqjE"
                     /locus_tag="ACHKW5_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7512738.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3579..3878
                     /gene="yqjK"
                     /locus_tag="ACHKW5_003403"
     CDS             3579..3878
                     /gene="yqjK"
                     /locus_tag="ACHKW5_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO7512739.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4064..4456
                     /gene="yqjF"
                     /locus_tag="ACHKW5_003404"
     CDS             4064..4456
                     /gene="yqjF"
                     /locus_tag="ACHKW5_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7512740.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4526..5512
                     /gene="yqjG"
                     /locus_tag="ACHKW5_003405"
     CDS             4526..5512
                     /gene="yqjG"
                     /locus_tag="ACHKW5_003405"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO7512741.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5805..6170
                     /gene="yhaH"
                     /locus_tag="ACHKW5_003406"
     CDS             5805..6170
                     /gene="yhaH"
                     /locus_tag="ACHKW5_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7512742.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6412..6768
                     /locus_tag="ACHKW5_003407"
     CDS             6412..6768
                     /locus_tag="ACHKW5_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7512743.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6819..7715)
                     /gene="yhaJ"
                     /locus_tag="ACHKW5_003408"
     CDS             complement(6819..7715)
                     /gene="yhaJ"
                     /locus_tag="ACHKW5_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO7512744.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7820..8521
                     /gene="yhaK"
                     /locus_tag="ACHKW5_003409"
     CDS             7820..8521
                     /gene="yhaK"
                     /locus_tag="ACHKW5_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO7512745.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8544..8708
                     /gene="yhaL"
                     /locus_tag="ACHKW5_003410"
     CDS             8544..8708
                     /gene="yhaL"
                     /locus_tag="ACHKW5_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO7512746.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8920..10230)
                     /gene="cyuA"
                     /locus_tag="ACHKW5_003411"
     CDS             complement(8920..10230)
                     /gene="cyuA"
                     /locus_tag="ACHKW5_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO7512747.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10258..11589)
                     /gene="cyuP"
                     /locus_tag="ACHKW5_003412"
     CDS             complement(10258..11589)
                     /gene="cyuP"
                     /locus_tag="ACHKW5_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7512748.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11864..13228)
                     /gene="tdcG"
                     /locus_tag="ACHKW5_003413"
     CDS             complement(11864..13228)
                     /gene="tdcG"
                     /locus_tag="ACHKW5_003413"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7512749.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13300..13689)
                     /gene="tdcF"
                     /locus_tag="ACHKW5_003414"
     CDS             complement(13300..13689)
                     /gene="tdcF"
                     /locus_tag="ACHKW5_003414"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO7512750.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13703..15997)
                     /gene="tdcE"
                     /locus_tag="ACHKW5_003415"
     CDS             complement(13703..15997)
                     /gene="tdcE"
                     /locus_tag="ACHKW5_003415"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO7512751.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16031..17239)
                     /gene="tdcD"
                     /locus_tag="ACHKW5_003416"
     CDS             complement(16031..17239)
                     /gene="tdcD"
                     /locus_tag="ACHKW5_003416"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO7512752.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17265..18596)
                     /gene="tdcC"
                     /locus_tag="ACHKW5_003417"
     CDS             complement(17265..18596)
                     /gene="tdcC"
                     /locus_tag="ACHKW5_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO7512753.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18618..19607)
                     /gene="tdcB"
                     /locus_tag="ACHKW5_003418"
     CDS             complement(18618..19607)
                     /gene="tdcB"
                     /locus_tag="ACHKW5_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO7512754.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19706..20644)
                     /gene="tdcA"
                     /locus_tag="ACHKW5_003419"
     CDS             complement(19706..20644)
                     /gene="tdcA"
                     /locus_tag="ACHKW5_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO7512755.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5362 a         5410 c         4930 g         5068 t
ORIGIN      
        1 acaatgacga tacttatttt ttaggaattt ataaatattc acaatattaa ttcaatacat
       61 taattcaatc accaaattta tatatgacca atggcatacc catgaataaa gtggtatgat
      121 aacttcgtcc gcattccact ccgggagacg catatggaaa tcactgaacc acgccgtttg
      181 tatcaacaac ttgccgctga cctgaaagag cgcatcgaac agggcgtcta tctggtaggt
      241 gataaactgc ctgcagaacg ctttattgcc gatgaaaaga acgtcagccg tacggtagtt
      301 cgtgaagcca tcatcatgct ggaagttgaa ggctatgtcg aagtgcgtaa aggttcaggt
      361 attcatgtgg tttccaacca gccgcgccat cagcaggcgg ctgacaataa tatggaattc
      421 gccaattacg gtccgtttga gttgcttcag gctcgccagc tcatcgaaag taatattgcc
      481 gagttcgcgg caactcaggt aacgaaacaa gacatcatga aactgatggc cattcaggaa
      541 caggcgcgcg gcgaacaatg cttccgtgat tccgagtggg atttacagtt ccacattcag
      601 gtagccctgg cgacgcagaa ctccgccctg gcggccatcg ttgaaaaaat gtggacccaa
      661 cgtagccata acccgtactg gaaaaaactg cacgaacaca ttgattcccg taccgtcgat
      721 aactggtgtg atgaccacga tcaaatcctc aaggcgctga ttcgcaaaga tcctcatgct
      781 gctaagctgg caatgtggca gcacctggaa aacaccaaga tcatgttatt caacgaaacc
      841 agcgacgact tcgagttcaa tgccgaccgc tatctgttcg ccgaaaaccc ggtagtgcat
      901 ctcgatacgg ccaccagtgg cagtaaatga aactcctgtc cgacaggcgc tgcgacgcgc
      961 ctgtttgtct cgtcggtaag cgaaaagtat aaagtgtcag cctgtgtaaa tcctctcgcc
     1021 accctcccct gcattcagca aaatcagact ccacggacat gtaattttga taacgaacaa
     1081 cgttgacctt tgttacaatt agattcaatt tgaatttatg tttttgaatg ctttcttatc
     1141 tcacgattta acagggaata gttcaggctg tgttgatgta tcaaacccgc agaacatacc
     1201 aaaacagcaa taacattgcg gtagtgcatc ttttaaaacc agcgtggcgt taaccgattc
     1261 accaggaata atgaatggaa cttttgaccc aattgctgca agccctgtgg gcgcaggatt
     1321 ttgaaaccct ggccaatcca tcgatgattg gcatgttgta ttttgtcttg tttgtaattt
     1381 tgttccttga aaacggcttg cttccagcgg cctttttacc gggcgacagt ttactggtat
     1441 tggtcggcgt gttgattgcg aaaggcgcga tgggttaccc gcaaacgatt ctgctgctga
     1501 ccgttgccgc cagcctcggc tgctgggtca gctatattca ggggcgatgg ctgggcaata
     1561 cccgcaccgt acaaaactgg ctatctcatt tacccgcgca ttatcatcaa cgcgcacacc
     1621 atctttttca taaacacggt ttatcggcgc tgttaattgg tcgctttatt gcgtttgtca
     1681 gaacactgct gccgacgatt gccgggttat cagggctgaa taacgcgcgc tttcagtttt
     1741 tcaactggat gagcggtctg ctgtgggtat tgatcctgac aactctgggt tacatgctcg
     1801 gcaaaacgcc ggtattttta aagtacgagg accagctgat gtcatgcctg atgctgctcc
     1861 cggtggtgct gctggtgttt ggcctggcag gttctctggt cgtgttatgg aaaaagaaat
     1921 atggaaatcg ggggtaaggg atgcaaatac ctcgcatgtc gcttcgccag ctagcctggt
     1981 ccggcactgt tttacttctg gtcggcacgc tgttgctggc ctggtccgcg gttcgccagc
     2041 aagagtctac gctggcgatt cgtgccgttc atcaaggcac aacgatgcca gacggttttt
     2101 cgatctggca tcaccttgac gctcatggca ttcctttcaa aagtatcacc cccaaaaacg
     2161 acactctgtt aattacattt gactccagcg accagagcgc cgccgcaaaa gcggtcctcg
     2221 acagaacatt gccccatggc tacatcattg cgcagcagga caataacagt caggctatgc
     2281 agtggctgac ccggttacgg gataactctc atcgcttcgg ataacttcct gttatccgaa
     2341 acatatcact cactttggtg atttcaccgt aactgtctat gattaatgag gcggtggtta
     2401 acactaccgc ttcgttgtct ggacaatcgt tcctttgtaa taggtccgaa tcacaatgga
     2461 aggttcaaga atgaaatacc gcatcgcttt agctgtttct ctctttgctc ttagtgccgg
     2521 tagttatgcc actaccctgt gtcaggaaaa ggagcaaaat atccttaagg agatcagcta
     2581 tgccgaaaaa caccaaaacc agaatcgtat tgacggtctg aataaagccc tgagtgaagt
     2641 ccgggccaac tgttcagata gccagctgcg tgccgatcat cacaagaaaa tcgcaaagca
     2701 gaaagatgag gtggcggaac gccagcaaga tttagccgag gcgaagcaaa aaggcgatgc
     2761 cgataagatt gccaaacgcg aacggaaact ggcagaagcg caggaagagc tgaaaaagct
     2821 ggaagcgcgc gactactaac tcacaatagt cactacttac tcacctggag aaaactatgt
     2881 cgaaagaaca cactacggaa catctgcgtg ctgagttgaa atccctttcc gatacgctgg
     2941 aagaggtgct tagctcatct ggcgagaagt cgaaagaaga gttgagtaag attcgtagca
     3001 aagcggagca ggcactgaaa cagagccgtt atcgcctggg tgaaaccggt gatgccatcg
     3061 ccaaacaaac ccgtgtcgcg gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga
     3121 ccggcgtggg aattggcgct gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt
     3181 aattatggcg gacactcatc acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca
     3241 gcgaattgtt tccatcatgg ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga
     3301 gctggaagag gaaaaagcta atctctttca acttttactg atgctgggcc tgacgatgct
     3361 tttcgctgca tttggtctta tgagcctgat ggtgctaatt atttgggcgg ttgatccgca
     3421 atatcgcctg aatgcgatga ttgccaccac cgtggtgttg ctgctactgg cactgattgg
     3481 cggtatctgg acgttacgta aatcgcgtaa atccaccttg ctgcgccata cacgccatga
     3541 gctggcaaac gatcggcaac tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg
     3601 aacgacgtaa ggcgcaactg cttagccaga tccagcaaca acggctggat ctttccgcca
     3661 gtcgtcgtga atggctggag gcaacaggcg cttacgatcg tcgctggaat atgctgctaa
     3721 gtctgcgctc ctgggcgctg gttggcagta gcgtgatggc gatctggacg attcgccatc
     3781 ctaatatgct ggtccgctgg gccagacgcg gttttggcgt atggagcgcc tggcgtctgg
     3841 taaaaacgac cctcaagcag caacagcttc gcggttaact tccctctggc cggagcaatt
     3901 ccggccttat ccctcaaatt ttttgaagat ttttgacagt tttccttgct aacaatcatc
     3961 attcaccacg tttatgattc tctccatcga cagcaacgac gctaataccg cgcccttgca
     4021 caaaaaaaca atcagcagcc tgagtggcgc agtggagagt atgatgaaaa aattagaaga
     4081 tgttggtgta ctggtagcgc gcattttaat gccgattctg tttattaccg caggctgggg
     4141 aaaaatcacc gcttactctg gtacccaaca atatatggaa gcaatgggtg tcccgggttt
     4201 tatgctgccg ctggtgattc tgcttgagtt cggtggtggt ctggcaatcc tgttcggttt
     4261 cctgactcgc accacagccc tgtttactgc gggctttacg ctgctgacgg catttttatt
     4321 tcacagcaac tttgctgaag gcgtcaactc gctgatgttc atgaaaaacc tgacaatttc
     4381 tggcggattc ctgctgctgg caattaccgg tccgggcgcg tatagcatcg accgcctgct
     4441 gaataaaaag tggtaagcac gcgctatact taacgataaa aaacgaggag gaagctcctc
     4501 gtttttgcta ttggaggaga gaaaaatggg tcaactgatt gacggcgtct ggcatgacac
     4561 ctggtacgat accaaatcta ccggcggtaa atttcaacgt tcagcttccg catttcgtaa
     4621 ctggctcact gccgatggcg cacctggccc cactggcaca ggcggtttta tcgcagagaa
     4681 agatcgttat catctctatg tttcactcgc ctgcccgtgg gcgcaccgca cgctgatcat
     4741 gcgcaaactc aaaggactgg aaccgtttat ttccgtttcc gtagtgaacc cgctgatgct
     4801 ggaaaacggc tggacctttg atgacagttt tccgggagca accggagata cgctctatca
     4861 acatgaattt ctgtatcagc tttatctcca cgccgatcca cactacagcg gacgagttac
     4921 tgttcccgtg ctgtgggaca aaaagaacca caccatcgtc agcaacgaat cagcagaaat
     4981 catccgcatg tttaataccg cgtttgatgc gctgggcgcg aaagcgggtg attactaccc
     5041 accagccctg caaacgaaaa ttgacgaact taacggctgg atttatgaca ccgttaacaa
     5101 cggcgtgtat aaagctggtt ttgccaccag ccagcaagcc tacgacgagg cggtggcgaa
     5161 agtgtttgaa tcgctggcgc gactggagca gattttaggc cagcatcgtt acctgaccgg
     5221 caaccagcta accgaagccg atattcgcct gtggaccacg ctggtccgtt ttgatccagt
     5281 gtatgtgacc cacttcaagt gtgataagca ccgcatcagc gattacctta atctgtatgg
     5341 cttcctgcgt gatatctacc agatgccggg aattgccgaa acagtcaatt tcgatcacat
     5401 ccgtaatcat tacttccgca gccataagac catcaaccct acggggatta tttcaattgg
     5461 tccatggcag gatctcgatg aaccgcatgg acgagatgtt cgcttcggtt aaaaaaaggc
     5521 accttacgag gtgcctctga taattagcta agtgcatgtt gttcatgtcg gatgcggcgt
     5581 gaacgcctta tcaaacctac aaaacattta tattcaatat gttgcaatga ccgcgcaggc
     5641 cagataagcg tagcgcatct ggcgattttg cgcttgtcat cagtcttagc gcgccttcac
     5701 agcactttat taattctttg aacgaatatt tactgccatt tcattcacgt ctattcttaa
     5761 tttgctgctt taaaaacaag tgattgagca aaattgaggc aaaaatggac tggtatctga
     5821 aagtactaaa aaattatgtc ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt
     5881 ttattctggt caacatcatc tttacgttcg tgctggggct gctggataaa atgttaggct
     5941 ggcaacgcgc tggtggcgaa ggcatcctga cgacaatcta cggtattctg gtgtttttac
     6001 cgtggtgggc agttcagttc cgccgcctgc acgacaccga ccgctcggcg tggtgggcac
     6061 tgttgttctt aatcccgttt atcggctggc ttattatcat cgtctttaac tgccaggcgg
     6121 gtacgccagg cgaaaaccgc tttggaccag atccgaaact ggaaccttaa aacaaatctg
     6181 ctcgccggat gtctgactca tccggctttt atcatcatgt tgatgaatga ataatttact
     6241 aatgtttatt taaaatattt caatgtcgtt atataaatta cctataaaaa acaaccatgt
     6301 atttatctta aatatcaaaa cctttataac atccgaaaaa gaaagatacg aatagcattt
     6361 acacaatacc cggaatagac tttcgtcact tttaattaaa gggatgtttt tatgcagtgg
     6421 tatttagccg tattaaaaaa ttatgttggt ttctctggtc gcgcacgtcg taaagagtac
     6481 tggatgttta ctctgattaa cgccatcgtc ggcgctatta tcaatgtcat tcaattgatt
     6541 ttaggtttag agtttccatt cctttctctc atttacctgg cagcaacaat cattccggtc
     6601 attgctctgt gtgttcgtcg tctgcacgat actgaccgtt caggtgcgtg ggcgcttctc
     6661 tatctggtac cgatcattgg ctggttagtt ctgtttgtat ttgcctgttt agaaggcaat
     6721 tctggcagca atcgttacgg aaacgatcct aagtttggtt caaattaatc ttagaattgg
     6781 ggcgacattt cgcccctttt tattaataat aacaaatatt attttccgct aaaaagtttg
     6841 ggaatttccc gcagacacca ggatttcgct tcccccatac tgtcacgacg ccaggccata
     6901 ataatatcga tctcgctggt cgattccggg ctgacgacac gcaaccgccc ttccgcaata
     6961 tctttttcga ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga
     7021 cgtttatctt caatcgtgct caccgttaag cgcggctgtt tgtccagcag ctgtacggtc
     7081 aacaccgggc gctcacgagc ggtatccgct accgcaattc cacgatattt cacgcgcgtc
     7141 acttcagata acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg
     7201 ttcattaagg tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc
     7261 ggcgcgataa caatatccgc ccgcccctgc tccagtcgtt cccacgcccc cgccagcact
     7321 tcggtgatga ttgccagttg ggtattggct tttgccgcca gtttgtcgat taacgggaaa
     7381 aaggcaggtg tcggtaccag cgcctcggtc acaatggtga gatgcgtttc ccaaccgcgc
     7441 gcgagggctt ccgcatcggt agtcagttta tctgcggctt ccagcaaaac gcgccctcgc
     7501 tccagcaaca tcctcccgac attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc
     7561 accacatcca gctcttcttc cagtttttgc atggtgtagc taagtgcgga aggcacgcgt
     7621 cccagctcat ccgccgccgc cgcaaaactg ccccggcgat cgatcgcatc cataacccgt
     7681 agtgcttcca gcgttaatgc cctttctttg gccatttcgt tctcattcag gaaatttgaa
     7741 cataccgggc agaatatctg gctaacaatg tagcgtccag ccccttacca taaaaggaag
     7801 taaagagagg tcaagaatta tgattactac ccgaactgcc agacagtgtg gacaagcaga
     7861 ctacggatgg ttgcaggccc ggtatacttt ttcctttgga cactacttcg acccgaaatt
     7921 gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag gtgccgcctt
     7981 tcagccgcga acctatccca aagtcgatat tttaaatgtg attctggatg gagaagcaga
     8041 gtatcgcgac agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt tgctgctctc
     8101 tacccagccg ggtgtcagct acagcgaaca caatctcagc aaagacaaac cgttaacacg
     8161 aatgcagctt tggctggacg cctgcccgca gcgagagaat ccgctgattc aaaaactggc
     8221 gcttaatatg ggcaagcagc aattaatcgc ctcgccagag ggtacgatgg gaagcctgca
     8281 attacgccag caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt
     8341 ccagttgcat gggccacgcg cgtatttgca atcgattcac gggaaatttc atgcgcttac
     8401 gcatcatgaa gagaaagcgg cgctgacctg cggtgatggg gcgtttattc gtgacgaggc
     8461 taacattacg ctggttgccg attccccact gcgcgctttg ctgatagatt tgcctgtcta
     8521 gttgttttta cagggagatg atgatgagta aaaaatcggc caaaaagcgc cagccagtga
     8581 agcccgtggt ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat gaagagatgt
     8641 tgagcgagct ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta
     8701 ccgcgtaaag acaggttgcc ggatgcggcg aaaacgcctt atccggccta caaaatccaa
     8761 caacattaac gaattaaaca acccgtaggc cggataagat gcgccagcat cgcatccggc
     8821 atcaggtaca aaacacaaca acatcaacaa gttgagcacc ccgtaggccg gataaggtgc
     8881 gccagcatcg catccggcac aatactcaaa acctggcgtt tatctggcct tgctcgccat
     8941 aatctcgata atctgccgat ccgtttgctg catcgaatgg cttgctaacg cacacaggtt
     9001 ggcaatcgac tgctcaacat catgcgccac gatcccttca ttgccggtca cggcggtatc
     9061 atccagcgcc attaacaccg ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc
     9121 gcagctgttc gacgcaccat cgcaaatcat gccgctgaca tcgccgatca tactgctgat
     9181 cgccatcgag atggtttcat aacgcccatc caccagccat gccatcccgg cggcggcccc
     9241 cattgctgcg gtcgttgcgg cacaaagtgc agacaaacgc ggtaactggt tatggatgta
     9301 aattgcgctc aaatgagaaa gcatcagcgc acgcgccagc cgttcatcat ccgctccgaa
     9361 gtgttctgct accaccacca caggcattgt tgcggtgatc ccctggttac ccgagccgga
     9421 gttactcata gccggaagcg tagcgccgcc catacgcgca tcggatgccg cgctggtacg
     9481 aatcacaatg gatgaagaga gatctttcgc cagcaaaccg cgctcgcact gtttttccag
     9541 cgtcgcgcca atatgcagcc cccacttacc gctcaaacct tcctgcgata acgcacaatt
     9601 tagcttcgcg gaatcgagaa taaagcggat cgccgcaaac gggacttcat tgacgaactt
     9661 caggatctca gccagcgtcg ttctggaaag caccgtcagc ggagactctt gctcgccctc
     9721 tgccacacac gcctgctggg taaacaccac accattgtgc gtctcaatat gcacaatgtt
     9781 ggtatgcccg ccgacgatgg tgacacacgc ccacttctca ccgttccaga ctttggcgcg
     9841 tgagaagagg atttcattgc aaggttcctg gatcttaacg gagactttcc ccgccgccag
     9901 cagtgctttg gcatcggcaa ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc
     9961 gttggcattt ccacctaacg cccccagcgc cgccgcaatc ggcagcccca ccattcccgt
    10021 gccgggaacg gtgacgccca gaccgttctt catcagattt ggcgaaaccc aggcttctac
    10081 acgttcaacc ggaccttcca gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat
    10141 cggttcagta catcccagcg ccggttttac ttcctcctga acggcgagga tgtaacgctg
    10201 ccataacgga tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc aggtaaatca
    10261 agaaaatgcc aggaacggag aaacacaaag caacaaacca gtgacgataa tcaggtacag
    10321 agacatccct ttgtatttgt gcaatgccgg tactttgtaa accagccacg ccgggatcag
    10381 gcaccctacc atgccgaaaa tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt
    10441 cagtacgatg gcgctccagg ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag
    10501 attttcgtta atcttctcgg caggcatctt gcgacgcagg atgttcatta cgatcccttg
    10561 cgttgcttcg cgaaagccta aatagacgcc aaagaatgca gtcatgacag caaagatatt
    10621 gagaatgacg ctgaccactt tcacccacgc tgcgccgtca ccgctaataa actgtgcggc
    10681 aatcgccagc gcggaaatat tctgctcata ggcttttacc gcttcgtcat gtcccatcgc
    10741 cagcgtgaac gacacggcgt agaaaaagac ggtgacaaac aaaatgccaa acgcgatatt
    10801 cattgcccgc aatgctttat gccgcgccac ttcaattgat ttttcccgcg agcgataaga
    10861 gatcaccatc gggcttaacg tctggataaa cagaatcgac gtcagggtaa acggcagcgt
    10921 aataatggcg tttttcacca gcagccccag cggcggtagc gaaccgacgt tatacagatg
    10981 ccacattccc accatcgaca cgcccagcgc cgcgaccacc agcagcttgg tcagcaccat
    11041 gccggtcgaa attttgaata acaatttctc gccgcgtgaa gagatcgcca ccagaatgca
    11101 aatcagtacc agaccataaa agggactgtc tgacagcaac ccttccgtca cgccgaaggt
    11161 atgcagatag gaagcactat cgttggtgat ggcggtggaa tagacgaaca tccaaatcac
    11221 cagcattacg aaatagagcg cacctaacag gatgccccag tttttaccta aataaccgct
    11281 aatgacactc gggtaatctt tacattctgg tgattctgcc agcgtattaa taaacaaccg
    11341 ctgaaacaga tacattgccg ggtaaccaat caccgatgag agcaaaaata cccacaatcc
    11401 catcaaaccg acctgcaccg ggagaaaaac aatccccgcg ccaatcgcca tcccgatact
    11461 cataatcacc cagccggtgt cagtactgtc gaatttgatc gcttctcgcc actcgctctc
    11521 actcattcca gcacgacccg ccggggtcga agcgtctgca atgacgcctt tattcgatgc
    11581 aatttccata atttctcgct caatattttg tagggcttat tatttttttc cgagccgcat
    11641 caaggcgata tgcggtctac gtttttgcag gcgagttatt agaataaaga aatgatacgc
    11701 gcgaggtcag agaaaatctt cacaatcaag actttgaaat aacggagatg gataagaatt
    11761 ttctactaaa ttaatcgcaa gagaatattc attctctatt tgcggcagat cacaaaaaaa
    11821 aggtgcacat ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt
    11881 taatggccag tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt
    11941 catacatcgt ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg
    12001 ccatccgcgc ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga
    12061 tttgtacctg tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt
    12121 ccgccgcatt gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgccg
    12181 ccattgaaca ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg
    12241 cgttcatttt atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc
    12301 gctcgtttac cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc
    12361 acgcaccgtt agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg
    12421 ccagcgcgta catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc
    12481 tggaaaccag ctgacgacgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca
    12541 gcacgccttc agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg
    12601 caaaccctgc gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc
    12661 cagatataga caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat
    12721 cgtaaggtac ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa
    12781 acccgccgcc gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag
    12841 tgatccgcat tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg
    12901 ctacaggaaa atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta
    12961 ctaactctat aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca
    13021 gtcccatgat aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccat
    13081 acagatcgac cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa
    13141 taaaactttt tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa
    13201 ttttgaaaat atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt
    13261 ggagcggaac gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat
    13321 agcttcaatt tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt
    13381 cgggtaggtc gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa
    13441 atcattcaga tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc
    13501 agcaaccacg atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc
    13561 cgggatctca ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc
    13621 taaatcaacg ccctgaacat aagggccgat tgcgcctggg gcacgttgcg tttcgataat
    13681 ctttttcata catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct
    13741 gttgctgttc acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca
    13801 ggttaggata tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt
    13861 tgacgttgag gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca
    13921 ggccgacaag gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg
    13981 agaaggtgta cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca
    14041 atgaggccac ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg
    14101 gtgttccggc gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag
    14161 aagtgatagt cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa
    14221 ttttcttcat aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt
    14281 tgccgtactg cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac
    14341 ggattggttt cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc
    14401 ccgcgatgcc gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag
    14461 cttcgtagct gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca
    14521 ccgccagcca gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt
    14581 cgtccatcag cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc
    14641 cgttaattgc gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt
    14701 gcttaccaat caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt
    14761 cagtacgcat cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg
    14821 cggcatattt tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt
    14881 ccggtgccgg ccccatagtg tgcagggtgt gcaaatagcg gaaggagttt ttggtcacca
    14941 gcgtacgacc gtccagcccc atcccgccga tcacttccgt cgcccagatt gggtcgccgg
    15001 agaacagcga atcaaattcc ggtgtacgca ggaagcgtac catacggatc ttcatgatga
    15061 agtgatcgat cagttcctgt gcctgctgct cattgagtac gccagcttta aagtcgcgct
    15121 caatgtagat atcgaggaac gatgccgtgc ggcccagcga catcgcgccg ccattttgcg
    15181 atttcactgc cgccagataa gcgaagtaga gccactgcac cgcttcctgc gcattctgcg
    15241 ccgggcgaga gatatcaaag ccatatttcg ccgccatttc ctgaatctgc aacagcgcat
    15301 gacgatgctc tgccagctcc tcacgcagac ggatggtggc ttccagatcc tcgccttttt
    15361 ccagacgaga ctggagatcg gcaaattgca gttcgcgttc acgtaccaga taactgatgc
    15421 catacagcgc tacgcggcga tagtcaccga taatgcgccc acggccatag ccatctggta
    15481 aaccggtcag cacgccagat ttacggcagc gcagcatatc cggtgagtaa acatcaaata
    15541 cgccctggtt atgggtttta cgcagatcgg taaacagata ttcaaattca ctgtccattt
    15601 cacggccata ggcgtggaat gaacttttaa tcatattaat gccaccgaac gggtgtagcg
    15661 cacgtttcaa cggcgcatcc gtttgcaggc caacaatttt ttccagcggc tggttaatat
    15721 atcccgcatc atgagcggta attgtggtgg caatattggt atcgaaatca accggcgcgt
    15781 gggttgcatt ttcgatacgg atgccttcca ttactttttc ccacaattcc gtggtggcag
    15841 gcgtcgcttc ggcgaggaaa gattcatcgc cttcatacgg tgtatagtta tgttgaataa
    15901 aatcgcggac attaatttcg tttttccagt ccgtaccttt aaagccaagc catgcgtcgg
    15961 cgtacagctt atcgctggta tcaatatcta ccttcatgaa aaataatctc tctacaatac
    16021 ttcaactaaa ttatgcaaat tctgcgggcg cgttaacttt gcctaaatga atggcatcca
    16081 aagcaatcat tttttcttcg ttagtcggaa taacggcaca aatgacacgc gcattttcac
    16141 tggaaacaat tcgctcacca caggagttag agcgattatt catttctgta tcaatctcta
    16201 cgcctaatac agccaaatgt tccatgacca gacgacgaat taagcttgaa ttctctccta
    16261 ttccgccggt gaatataatt ccatccaggc gacgtaatga agctgcgtgt ccggcaatat
    16321 gacgggcaat tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat
    16381 gccaggcttt ttccagaaca cgtaaatccg aagaaagacc ggaaatacct aataatcccg
    16441 actctttatt cactacgcgt tccaggtcac ccaggctctg gttggtttgg ctggcgaccc
    16501 aggacatcgc accaaagtcg acatcgccac tgcgggtacc catcatcaag ccttccagcg
    16561 gcgtcattcc cattgaagta tcaacactct gaccgttgcg aaccgcgcag attgacgcgc
    16621 cattgccaag atgcgccaca accaggccgg agtcatcttc cgccagattc agcagcgaat
    16681 gtgcgcgctg ggaaacatag cggtgcgacg tgccgtggaa accataacgg cgtacaccta
    16741 actcttcata atatttccac ggcaggccgt ataaataagc ttccggagcc atcgtctggt
    16801 ggaaactggt atcaaatacc gccacctgag ttacgcccgg aaataattgc tgcgccgatt
    16861 caataccact taaattggcg taattatgca ggggtgccag tggagaaacg cgacggatat
    16921 tatcaatgac ttcatcggta ataatggcgg actcggtaaa aatactgccg ccgtgagcga
    16981 tgcggtggcc aattaaggcc acactgtcat ttaaattccg tttttccagt tcaaatgcaa
    17041 ttgccttcaa tgcaccttcg tagctgtggt gagccagcgg tgctggctct cccccattta
    17101 cggataagaa tgcattttcc gagttaatac cgtcggcaat acctgacatt aatacttcac
    17161 agtcgctggc atcgagtacg gaaaacttaa tcgaagacga accacagtta ataaccaaaa
    17221 caaccggaaa ttcattcatc tcttttctca tcctgagtta cggattaaaa cagtttgtat
    17281 acgatgttca ggatggtcag cagaccaatt acggtaacaa acacgttatc cagacgacca
    17341 cggtatttcg ccagagacgg cgctttacgg atggcataca tcggcaacag gcacagcagg
    17401 gatgcgataa tcggtgcgcc catggcttca ataaggtcga ggatgttcgg gttggcgtag
    17461 gcaacaaccc aggtggagcc catgatgaag atcatgctga tagtgttcag tttacccagc
    17521 gacactttag ttttgtcgcc tttataaccg aacttcagaa tcagaccatt caagccttcc
    17581 agcgtcccca gatagtgacc gaagaaagat ttgaagatag ccacgagtgc gatgatggaa
    17641 gccgcatatt ccagtgtaat cgcgaacgtt gttttggtac cggtcatgga cgcaaagtgg
    17701 ttagccagat aagaaagcac tggaatattc tgcgctttgg cttccgccat gttggccgga
    17761 gacagcgtaa acaggcagct aaaggcaaag aacatcacca ctgcaaccat cagcatgctg
    17821 gcacgagaaa tgatttggga acatttacgt tcggtgaagt cgcgaccgaa gtctttctca
    17881 tactcttcac gtttagaaac cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa
    17941 accatgatgg aaatccccag ccagacagtg atcaggatac cgtcatgacc ggttaacgac
    18001 agcgaaccga ggtcaacctg gtcgataact gcagagttcc agtaagggat cagcgacaga
    18061 gaaatcagca ccaggctggc gataaacggc cataccaggt agctcatcac tttaaccatc
    18121 agatccttac caaaccagat gacgaaagcc atcagcagca acaggaacag cgccacaaag
    18181 ccgcgattca gcggtgcaaa tccgagctgg ttttcccaga acgtcataaa ggtattggta
    18241 atagtaacgc cataaatcca cagcagtggg caaatcgcga agaagtacag gaacgtgata
    18301 accacgccgc cagttttacc aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg
    18361 ttagagccag aaagacacag acgcgccagc gcccggtggc aataaaatgc gatggggtat
    18421 gccaatacca acatcagaag aatcgggatc agtccgccaa aacctgcgcg gatagggaag
    18481 aacagcaccc cggcgccgat tgccgtacca aacaagccta acgtccatgt ggtatctgat
    18541 ttacgccagg acgattgttt tgtctggctg gatacaatgc tatctgaagt actcatatcc
    18601 tatcctcaac gaattaatta agcgtcaacg aaaccggtga tttgagagac gcgagaaaga
    18661 tcgatattgc cgccggaaat aatactgacg gtttttctgt tttgaatata ttggtctaat
    18721 ttaccgctta ataatgcagc acatgccaga gcgcctgcgc cttcggtgac gactttattg
    18781 cgctgaatta aggcaatcat actgtttctg atttcgtctt cgctgaccag cacgatgtca
    18841 tcgactaatt cacgaacgat ttcgtaagtt aaattacccg ggcgggagac atcacaacca
    18901 tccgccaggg tgccggtagt tcggtgcgtg gttatttctc cggagtggaa agaagccgcc
    18961 atgccgtgaa cgttttctga ctgtacgcca ataacacgaa tggtcgggtt aatagattta
    19021 attgccaccg caataccagc aattaaacca ccaccaccaa ttggcacaat cacgttatcg
    19081 acatcataga gatcttccat aatttccaga ccaatcgttc cctggccagc aatcactttc
    19141 ggatcatcgt aaggtgggat aaaaatacga ccttccattt cgacaatttc gctcacttta
    19201 gcgatagtgt cgttgaaatt atcaccatgc agaacgactt ctgcggagta gtcgcacgtt
    19261 gccgctactt tggattttgg cgcacctttt ggcatcacca ctttaccgtc gatacccagc
    19321 atcgcgcagg agagcgaaac cccttgcgca tggttgcccg cagaacaggc cactacacct
    19381 ttacgttttt ccgcatcaat cagtgaactt aatttattaa atgcgccacg aattttaaat
    19441 gaacccgtac gctgcatatt ttcaaacttc aggaatattt cacctttgca acgttcacta
    19501 aaatagtttg agcgaggcat gcctgtttta taaattcgcc cagccagtcg ttgtttcgct
    19561 tcaataatgt catcaatagc aaccggcaga tcgtatgtaa tatgcattat aaaacctctt
    19621 cgcctgaatt aaatatgtag gtaaccgtaa ccgacacctg caaaacagac aggtggatta
    19681 tttatgttta aataaaaaca aataactaac caacttctat taattgcctt cgtctacacc
    19741 cattataaga tgaatactct ttggctaatt ccaccaaaac cgatgctgct tttttaatac
    19801 gataattttt cgaccatacc gcggcatatt gtgccacagg taatgtttct tcaaccggaa
    19861 tagtaataaa ttgatttgaa ccaaaaggtg acgtcatatc acaaggaatt acagttaaga
    19921 aatcagcatt gagaacaaga ttataaattg tcacgactga gtcggtttta acgatgtttt
    19981 caatactgat gccatttctt tgtaacgtag taagaagctc gctgtagtac cccatattgg
    20041 tttgtggcaa cacccactgt tcgttcttca acgactccag cgtggtggtg ccggtgcatg
    20101 ttcgggactt actggctacc aacacaaact cggactcgaa cagcggctca acatgtaaat
    20161 cctgaagctt catttctgca cttaacgtac caatcgcaaa atccagtcga ccgtcgcgga
    20221 ttgccggtaa gaacgaagac agttgcgctt catacataga aacttgcgct ttcgggaaca
    20281 cctctttgaa cttgttgatc atccctgaca taaaagtaaa accaatcaat gaaggaaaac
    20341 caaatgagac ttccaccacc gcctcagaag acataccact tatctcatta accatatttt
    20401 tcatttcacg ggtaatggat tcggaacggg agagtaacaa ttgaccggca ggtgttaatg
    20461 ttacaccggt atttttccgc accactaatt ccacaccaaa ataatcttca atatcgttaa
    20521 tgattttact gacggctggt tgagttaacc ctaattcttt tgcagccgag ccgatagagc
    20581 cacttctaat gacttcctga aagactacca ggtgctgcgt tttcggaaga agaatagtgc
    20641 tcataacgac ctacgttaat tacctcattg acggcatgaa gtgtatcaaa atgaaatgaa
    20701 caggatatgt gcgaccactc acaaattaac tttcaatact ttccagggta tcgttattac
    20761 aattaaacac
//
LOCUS       DAZFYC010000084        20577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFYC010000084 DAZFYC010000000
VERSION     DAZFYC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.084"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(163..2154)
                     /gene="tkt"
                     /locus_tag="ACHKW5_003420"
     CDS             complement(163..2154)
                     /gene="tkt"
                     /locus_tag="ACHKW5_003420"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7512756.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2432..3190
                     /gene="loiP"
                     /locus_tag="ACHKW5_003421"
                     /gene_synonym="yggG"
     CDS             2432..3190
                     /gene="loiP"
                     /locus_tag="ACHKW5_003421"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO7512757.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3276..4838)
                     /locus_tag="ACHKW5_003422"
     CDS             complement(3276..4838)
                     /locus_tag="ACHKW5_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7512758.1"
                     /translation="MERTLPWKPLLCLLFLLSTGCDRAEKQKIAAQLQQDKEAISSVV
                     KKSYRALCGPEFVTSTDSHDRLAEMQQVFTPALMEPVLKYWQESPKTCKRRFPSYSDP
                     LATFVEGDKAQIGAFFGHQSYIDIPMELIREGDGKWKIAAIDFASLHEFEKNGRVTDE
                     VQKKIDLLKKRSFKQHRDFPLLTRTAPERVSNSPLTSVEQTVTEPDSSWLDVLLTIGK
                     YALYLVAIILILALVAVLWIGYGLVFAGHRLFKASCKDPSIQKIPAAERWAMAVGAPY
                     AIAGNNHWARRVVNDDSVEAENDCKSEVESLADMWGVFDRDSLLEQLLALFVAGHRSV
                     YAEQIKNDSEMPDAEYRAFAQQLAQNAKSSSEAKERLWQLQMVRKNRRKICDIDFCAW
                     DMVRFVMLCNSGAQVGYITQREMVDFSMLAARRVQQHYRSWRELAGHFLLARWYWKAT
                     DKRHLITHILFKKAITSLLKEQGSPWRTLPWDTPLPEGTETAFAWACGVSTADVVEVQ
                     PDTPDKAIYEAN"
     gene            complement(4989..5909)
                     /gene="speB"
                     /locus_tag="ACHKW5_003423"
     CDS             complement(4989..5909)
                     /gene="speB"
                     /locus_tag="ACHKW5_003423"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO7512759.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6046..8022)
                     /gene="speA"
                     /locus_tag="ACHKW5_003424"
     CDS             complement(6046..8022)
                     /gene="speA"
                     /locus_tag="ACHKW5_003424"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO7512760.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8031..8162)
                     /gene="yqgB"
                     /locus_tag="ACHKW5_003425"
     CDS             complement(8031..8162)
                     /gene="yqgB"
                     /locus_tag="ACHKW5_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO7512761.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8256..8399)
                     /gene="yqgG"
                     /locus_tag="ACHKW5_003426"
     CDS             complement(8256..8399)
                     /gene="yqgG"
                     /locus_tag="ACHKW5_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO7512762.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8510..8761)
                     /gene="yqgD"
                     /locus_tag="ACHKW5_003427"
     CDS             complement(8510..8761)
                     /gene="yqgD"
                     /locus_tag="ACHKW5_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO7512763.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8817..9971
                     /gene="metK"
                     /locus_tag="ACHKW5_003428"
     CDS             8817..9971
                     /gene="metK"
                     /locus_tag="ACHKW5_003428"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO7512764.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10407..11801
                     /gene="galP"
                     /locus_tag="ACHKW5_003429"
     CDS             10407..11801
                     /gene="galP"
                     /locus_tag="ACHKW5_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO7512765.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11878..12375
                     /locus_tag="ACHKW5_003430"
     CDS             11878..12375
                     /locus_tag="ACHKW5_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO7512766.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12470..13177
                     /gene="endA"
                     /locus_tag="ACHKW5_003431"
     CDS             12470..13177
                     /gene="endA"
                     /locus_tag="ACHKW5_003431"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO7512767.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13257..13988
                     /gene="rsmE"
                     /locus_tag="ACHKW5_003432"
     CDS             13257..13988
                     /gene="rsmE"
                     /locus_tag="ACHKW5_003432"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO7512768.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            14001..14951
                     /gene="gshB"
                     /locus_tag="ACHKW5_003433"
     CDS             14001..14951
                     /gene="gshB"
                     /locus_tag="ACHKW5_003433"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO7512769.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15060..15623
                     /locus_tag="ACHKW5_003434"
     CDS             15060..15623
                     /locus_tag="ACHKW5_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO7512770.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15623..16039
                     /gene="ruvX"
                     /locus_tag="ACHKW5_003435"
     CDS             15623..16039
                     /gene="ruvX"
                     /locus_tag="ACHKW5_003435"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO7512771.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16228..17208)
                     /locus_tag="ACHKW5_003436"
     CDS             complement(16228..17208)
                     /locus_tag="ACHKW5_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO7512772.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            17226..17930
                     /gene="yggS"
                     /locus_tag="ACHKW5_003437"
     CDS             17226..17930
                     /gene="yggS"
                     /locus_tag="ACHKW5_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO7512773.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17948..18514
                     /gene="yggT"
                     /locus_tag="ACHKW5_003438"
     CDS             17948..18514
                     /gene="yggT"
                     /locus_tag="ACHKW5_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO7512774.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18511..18801
                     /gene="yggU"
                     /locus_tag="ACHKW5_003439"
     CDS             18511..18801
                     /gene="yggU"
                     /locus_tag="ACHKW5_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO7512775.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18809..19402
                     /locus_tag="ACHKW5_003440"
     CDS             18809..19402
                     /locus_tag="ACHKW5_003440"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO7512776.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19395..20531
                     /gene="hemW"
                     /locus_tag="ACHKW5_003441"
     CDS             19395..20531
                     /gene="hemW"
                     /locus_tag="ACHKW5_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO7512777.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         4983 a         5434 c         5255 g         4905 t
ORIGIN      
        1 caaatgtcaa tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat
       61 atttgtgatc tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa
      121 taaaaaaggt cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc
      181 tttcgcaacg acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc
      241 tgactcaccg aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca
      301 gtagtcagca atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga
      361 ttcacggtaa gcagcatcct gcttgtcaaa tgcgtcggta gacggcatgg acaccacgcg
      421 cgctttcacg ccttcggcag tcagtttttc gtaggcagca acagccagtt caacttctga
      481 accggtagcg atgaaaatca gttccggctg accggcgcag tctttcagca cataaccacc
      541 gcgcgcgatg tttgccagtt gctcttcagt tcgttcctgc tgcgccaggt tctgacggga
      601 gaggatcagt gcggtcgggc cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga
      661 ttcaacctgg tcacacggac gccatgtaga catgttcggg gttacgcgca gagaagcgac
      721 ctgctcaacc ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta
      781 aaccatcacc tgacgctgtt tcatcagcgc agccatacgt acggcgttac gtgcgtattc
      841 cacgaacatc aggaaggtgg aggtgtacgg caggaaacca ccgtgcaggg agataccgtt
      901 agcaatcgcg gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc
      961 ttcgttgatt gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga
     1021 gccgccgagg aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt
     1081 acggctggcg attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc
     1141 gtcgaagtca gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg
     1201 ataagctttc gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc
     1261 ttctttcgca tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca
     1321 gcccagctgt tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc
     1381 gtgagtaccg gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga
     1441 cggtttgtca gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg
     1501 accgtcgatg tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc
     1561 ggtgaaccag ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat
     1621 cagtttaccc agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat
     1681 catgcagccg tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg
     1741 gttaaactgc gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc
     1801 ctgacccagc ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc
     1861 cggagtttta gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc
     1921 ggtgaggtgc agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg
     1981 gtcacggtca gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac
     2041 ttcggcaatg tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac
     2101 tgcgtccatg ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac
     2161 tccagatcgg atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta
     2221 cgccggaaaa tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt
     2281 ttgccgcttc agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg
     2341 ctgcgctttt ccttatactg agactgagcg tcggttcacc tgcaaacggc gcatttttag
     2401 aataatcctg accttgtgcg gaagaaaaaa catgaaaatt cgcgccttat tggtagcaat
     2461 gagcgtggca acggtactga ccggttgcca gaatatggac tccaacggac tgctctcatc
     2521 aggagcggaa gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga
     2581 tcaggcatgt caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc
     2641 taaacgtctg acaactattg ccaatgcgct aggcaacaat atcaacggtc agccagtgaa
     2701 ttacaaagtg tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg
     2761 cgtctatagc gggctgatgg atatgatgac ggataacgaa gtcgaagcgg tgatcggtca
     2821 cgagatgggg cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac
     2881 aaatgccgtg cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc
     2941 acaacttggt gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc
     3001 agaagccgat gattattctt acgatcttct acgccaacgc ggcatcagcc cggcaggtct
     3061 tgccaccagc tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga
     3121 cgaccatccg gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg
     3181 gataaagtga tttcatattt ctctgacagg aatgtcggag aaattttcat tcagagccgg
     3241 ccagagtcat taaacaagct caatcatttg gggcatcaat tcgcttcgta gattgcttta
     3301 tctggtgtat ccggttgcac ctctactaca tcggcagtgc ttacgccaca agcccaggca
     3361 aaagccgtct ctgttccttc aggtaatgga gtatcccagg gtaaagtacg ccacggactc
     3421 ccctgctctt ttaataaaga tgtaatggct ttcttaaaaa gaatatgggt aatcaggtgc
     3481 cgtttatccg ttgctttcca gtaccaacgg gccagcaaaa aatgcccggc caattcgcgc
     3541 cagcttctgt aatgttgctg tacgcgtctg gctgccagca tcgaaaaatc gaccatttca
     3601 cgctgggtaa tgtatcccac ttgcgccccg ctattacata gcatgacaaa acgcaccata
     3661 tcccaggcac aaaaatctat atcgcatatt ttacgtcgat tttttctgac catctgtagc
     3721 tgccacaaac gctctttcgc ttcgctactt gatttcgcgt tctgagctaa ttgctgagcg
     3781 aacgcgcgat actcagcatc cggcatttcg ctatcgtttt tgatctgttc tgcataaact
     3841 gaacggtgcc ctgctacaaa caaagctaag agttgctcaa gtaacgaatc ccgatcaaag
     3901 acgccccaca tgtcagcaag tgactcaact tcacttttgc aatcattctc tgcttccacg
     3961 gaatcatcat taacgactcg tcgcgcccag tgattgttac ccgcaatggc ataaggcgcg
     4021 ccgactgcca ttgcccaacg ttcggcagca ggaatttttt gaattgaggg atctttacag
     4081 gatgctttga aaaggcgatg tccggcgaac accaggccat aaccaatcca caaaacagcc
     4141 accaaggcga gaataagtat tatcgctacc agataaaggg catatttgcc tattgtcagt
     4201 aagacatcca gccaggaaga gtctggctcc gtaactgttt gctccaccga tgtcaaaggg
     4261 gaattactga cccgctctgg cgcggtcctc gtcaacagag ggaaatccct atgttgcttg
     4321 aatgagcgtt ttttaagcaa atctattttt ttctgcacct catcagtaac ccgaccgttt
     4381 ttttcaaact catgtaacga tgcaaaatca atcgccgcaa tcttccattt accatcccct
     4441 tcacggatta attccattgg aatatcaata taagattggt gaccaaaaaa agcaccaatc
     4501 tgcgccttgt cgccttcgac gaaggtcgct aaaggatcgc tataactggg gaaacgtcgt
     4561 ttacacgttt tgggtgactc ttgccagtat ttcagtacag gctccattaa agcaggcgta
     4621 aatacttgct gcatttccgc aaggcgatcg tgcgaatctg tgctggtgac aaactcaggg
     4681 ccacataatg cgcgataact ctttttgacg acagaggaaa tagcttcttt gtcttgttgc
     4741 agctgtgcgg caattttctg tttttctgct ctatcgcagc cagttgataa aagaaataac
     4801 aaacaaagaa gaggcttcca gggaagagtg cgttccatct atttattcca ttaaaaacac
     4861 ggtaataaca atccattaaa aaaacagcag tcagggtaaa gatgacttaa ttaacaggat
     4921 aggttttaaa tgataagtcc cttgtgccag acaagggact gtgcaaaaaa ttcgaccagg
     4981 caggttactt actcgccctt tttcgccgcc tgaatataca gcatttccag cgccagcgtt
     5041 gccgctgcca gagcagtgat ttccgactga tcgtatgccg gagccacttc cactacgtcc
     5101 atcccaacga tgttgagatc tttcaggccg cgtaccagtt taatggcgcg atcggaggtc
     5161 aggccgccaa tcactggcgt accggttccc ggcgcgaaag caggatccag gcagtcgata
     5221 tcaaaggtca ggtaaaccgg catatcaccc acaatctgtt tcacttgggc gataacgtca
     5281 tccacgctgc gatcgttcac ctggcaggcg tccagcacgg taaaaccgtt gtctttatca
     5341 aactcggtac gaataccaat ctgcacggaa tgattcgggt cgatcagacc ttctttcggc
     5401 gcggtataga acatggtgcc gtggtcaaat tcacaaccgt tcgcgtaggt atcggtgtgg
     5461 gcgtcaaagt gtaccagcgc cattttaccg aaatgcttcg catgagcacg cagcagcggc
     5521 agcgtaacaa agtggtcacc accgaaagag agcatacgct taccggcagc cagcagcttc
     5581 tcggcgtgtg cctgcagttt ttcgctcatc tcacgggcat cgccaaaggc atataccaga
     5641 tcgccgcagt ccacgacgtt caggcgctca cgcatgtcga aattccacgg gaagcgattg
     5701 tgttcccagg caagattcgt cgaaacctga cggatcgctg ccggaccgtg gcgtcccccc
     5761 gcacgaccag aagtggccat atcgaacggc acaccagtaa tcacccagtc agcatcgctg
     5821 tcatacggct ggaagttcat cggcaggcgt aaaaaaccaa aggcgttgga aaccagtgag
     5881 ttatcgtatt gatgacctaa ggtgctcatg gatggactcc tattacaaag tcgatataaa
     5941 aaaacccttc cgcgtcgtta ggcccgacga ggaagggttg gatttgtcac aataaattgt
     6001 ggcggattat caccgctaaa ttaagcggat tcaagtaata cggacttact catcttccag
     6061 ataagtataa ccgtacaaac ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc
     6121 atcaagatcg gttttcttca cttgatcgcg gaactgagtt aacagcgttt tcggatcgag
     6181 ctgcacatat tgcagcatat ccgcaacggt atcgccttcg tcagacagtt ccacttctac
     6241 actaccgtca gggaagacga acacgtcaac cgcttcggta tcaccgaaca ggttgtgcat
     6301 gttgccgagg atctcctgat atgcgccgac cataaagaaa ccgagcatcg gcggattctc
     6361 tggatcgtac tccggcattg gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc
     6421 gatagcaccg tcagagtcac aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg
     6481 atccagccct tccagcggca gaaccgggaa caactggtcg atcccccatg cgtccggcat
     6541 tgactggaac agcgagaaat tgacgtacat tttgtccgcc atacgttcct gcagttcgtc
     6601 gattatcgga cgatgcgcac ggttttgcgg atccagttgc ttctgcactt catggcacat
     6661 gctcagatag agttgttctg cccatgcacg ttcttgcagg ctaaaggtgc cggaagagta
     6721 gccgatatga atgtcgtgca gatccatctg actgtcgtgt aaccattcac gcagagaacg
     6781 gcgagttccc ggttcgtgca tctcctgcca ggtttcccac atgctttgca gcgcgcgcgg
     6841 cgcatcttct gcaggcgcgg tcggcaccgt gtattcgtta cgttccacgc cgatgatatt
     6901 agacaccagc acggtgtgat gcgcagtcac cgcacgaccc gattcggtga ttaccgtcgg
     6961 atgcggcaga ccgttttctt cacacgcatc accaatcgcc cagatgatgt tgttggcata
     7021 ttcattgagg ccgtagttta cggagcagtc agactgcgaa cgggtgcctt catagtccac
     7081 gcccagaccg ccgccgacgt cgaagcactg aatattgacg cccagcttgt gcagttccac
     7141 atagaaacgc gccgattcac gaacgcctgt ggcgatatcg cgaatattcg ccatctgcga
     7201 accgaggtgg aagtgcagta gttgcaggct gtcgagacgc ccggcttcac gcagggtttc
     7261 aaccagttgc agtacctgag tcgcagccag gccgaacttc gatttttccc cgccggagga
     7321 ctgccattta cccgagccct gcgaagccag acgtgcacgc acgcccagac gaggaacgac
     7381 attcagacgt tctgcttcat ccagcacaat ggcgatttct gacatcttct caatgaccag
     7441 atagaccttg tgccccatct tctcgccaat taatgccagg cggatatatt cgcggtcttt
     7501 ataaccgttg cagacgatga cgctacgggt catgccagca tgtgccagca ctgccatcaa
     7561 ttcggctttg gaaccggctt ccagacccag cggttcgccc gaatgaatta gggactcaat
     7621 cacgcgacgg tgctggttaa ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc
     7681 gtaggattcc ctcgcacgtt tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat
     7741 ctgtgggaaa cagaacagtg caggcagacg ctgaccctgt gcttcacgag ttttcactaa
     7801 ctgcgcgaga tcgacgcgag cttccgggac gtccgggtcc gggcacacgc tgatgtggcc
     7861 cagctcgtta acgtcatagt agttattgcc ccaccaggca atattgtaag tacgcagcat
     7921 cttgctggct tcctgggagc tcattgcaac ctcctgcatg gagcgtagta caccgtgttc
     7981 gcccgctgac gaaggcaaac ccatagacat gtcgtcagac atagcgaacc tcaaattatt
     8041 ttattaagtg taaaacagtt aacgactatc gcagcctgga actgcgataa caacccataa
     8101 gcacatggat tttccagcag tgaatgctga cgctccaact gcgcaaccgg tttctttttc
     8161 ataacattat taagcacata accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa
     8221 actcttgttt ttacaagagc gcccttgttc agtcctcagt aactgtaacc agctcttgaa
     8281 tcctgagaag cgccgagatg ggtataacat cggcaggtat gcaaagcaga gatgcagagt
     8341 gcggggaacg aatcttcacc agaacggtga gacaggttaa gcagcagaca acggttcatt
     8401 atttcgtatc acctccacgg ccgcctgtta agacgaaccc acaagccaaa actctgattt
     8461 caacccggct ggaagtggca acacgaaaga aacgtcgtgt gctttttatt taagccgcgc
     8521 gccgcgtttt ataccccaca atggcagaaa attgcaaaag ataaatacgc agaatgccgg
     8581 cattgtcagg aaaaatttcc agccacgttt ttaacagaat gagacacgat tcaaaaaaaa
     8641 gtggaaatcg ggtgaagaat tgacctaaaa tagccatcca gatgttaatc catccatacc
     8701 gattaacact cagactgcca gtgtttttaa cttgcagagt cgtggtagga tccgctacca
     8761 cagaaaatcc acacaacagt ttgagctaac caaattctct ttaggtgata ttaaatatgg
     8821 caaaacacct ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc
     8881 aaatctctga tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt
     8941 gcgaaaccta cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc accagcgcct
     9001 gggtagacat cgaagagatc acccgtaaca ccgttcgcga aattggctat gtgcattccg
     9061 acatgggctt tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg
     9121 acatcaacca gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc
     9181 tgatgtttgg ctacgcaact aatgaaaccg acgtgctgat gccagcacct atcacctatg
     9241 cacaccgtct ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc
     9301 gcccggacgc gaaaagccag gtgactttcc agtatgacga cggcaaaatc gttggtattg
     9361 atgctgtcgt gctttccact cagcactctg aagagatcga ccagaaatcg ctgcaagaag
     9421 cggtaatgga agagatcatc aagccaattc tgcccgctga atggctgact tctgccacca
     9481 aattcttcat caacccgacc ggtcgttttg ttatcggtgg cccgatgggt gactgcggtc
     9541 tgactggtcg taaaattatc gttgatacct acggcggcat ggcacgtcac ggtggcggag
     9601 cattctctgg taaagatcca tcaaaagtgg accgttccgc agcctacgca gcacgttatg
     9661 tcgcgaaaaa catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg
     9721 caatcggcgt ggctgaaccg acctccatca tggtagaaac tttcggtact gagaaagtgc
     9781 cttctgaaca actgactctg ctggtacgtg agttcttcga cctgcgccca tacggtctga
     9841 ttcagatgct ggatctgctg cacccgatct acaaagaaac cgcagcatac ggtcactttg
     9901 gtcgtgaaca tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg
     9961 gtctgaagta atctttcttc acctgctttc aaaggccagc ctcgcgctgg cctttttctt
    10021 ttacctcacc gcggtggata ggcattcacg ccgcatccgg caaaaagctg cccgcacaat
    10081 aacatcattc ttcctcaaca cgttttatcg caaattatca tcactactga aaccgattac
    10141 accaataaca ccagccaaat atttgtaata ttttcatatt attattcagt tttcacagtt
    10201 gttacatttc ttttcagtaa agtcttaatt gcagataaca gcgtttaatc tatgatgata
    10261 taactcaatt attttcatgc acttaaacca taactaagat aaatgttagt gtaagcgatt
    10321 acactgatgt gatttgcttc acatcttttt acgtcgtact cacctatctt aattcacaat
    10381 aaaaaataac catattggag ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca
    10441 aggcaatgac gtttttcgtc tgcttccttg ccgctctggc gggattactc tttggcctgg
    10501 atatcggtgt aattgctggc gcactgccgt ttattgcaga tgaattccag attacttcgc
    10561 acacgcaaga atgggtcgta agctccatga tgttcggtgc ggcagtcggt gcggtgggca
    10621 gcggctggct ctcctttaaa ctcgggcgca aaaagagcct gatgatcggc gcaattttgt
    10681 ttgttgccgg ttcgctgttc tctgcggctg cgccaaacgt tgaagtactg attctttccc
    10741 gcgttctact ggggctggcg gtgggtgtgg cctcttatac cgcaccgctg tacctctctg
    10801 aaattgcgcc ggaaaaaatt cgtggcagta tgatctcgat gtatcagttg atgatcacta
    10861 tcgggatcct cggtgcttat ctttctgata ccgccttcag ctacaccggt gcatggcgct
    10921 ggatgctggg tgtgattatc atcccggcaa ttttgctgct gattggtgtc ttcttcctgc
    10981 cagacagccc acgttggttt gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc
    11041 tacgcctgcg tgacaccagc gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt
    11101 tgcaggttaa acagagtggc tgggcgctgt ttaaagagaa cagcaacttc cgccgcgcgg
    11161 tgttccttgg cgtactgttg caggtaatgc agcaattcac cgggatgaac gtcatcatgt
    11221 attacgcgcc gaaaatcttc gaactggcgg gttataccaa cactaccgag caaatgtggg
    11281 ggaccgtgat tgtcggcctg accaacgtac ttgccacctt tatcgcaatc ggccttgttg
    11341 accgctgggg acgtaaacca acgctaacgc tgggcttcct ggtgatggct gctggcatgg
    11401 gcgtactcgg tacaatgatg catatcggta ttcactctcc gtcggcgcag tatttcgcca
    11461 tcgccatgct gctgatgttt attgtcggtt ttgccatgag tgccggtccg ctgatttggg
    11521 tactgtgctc cgaaattcag ccgctgaaag gccgcgattt tggcatcacc tgctccactg
    11581 ccaccaactg gattgccaac atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc
    11641 tgggtaacgc caacaccttc tgggtgtatg cggctctgaa cgtactgttt atcctgctga
    11701 cattgtggct ggtaccggaa accaaacacg tttcgctgga acatattgaa cgtaatctga
    11761 tgaaaggtcg taaactgcgc gaaatcggcg ctcacgatta atctcctcag gcttcctccc
    11821 gtcgcggagg aagccacctc ttgcagtcat cttttcttcg ctctatcctc tgccgctatg
    11881 aaaacatccc gtctccctat cgccatccaa caggccgtta tgcgtcgcct gcgggaaaaa
    11941 ctcgcccagg ccaacctgaa gctagggcgt aactacccgg agccaaaact ctcttacacc
    12001 cagcgcggaa cctccgccgg aacggcctgg ctggaaagct atgaaattcg cctcaatccc
    12061 gttttgctgt tggaaaacag tgaagctttt attgaagaag tggtaccgca cgaactggca
    12121 catttgctgg tatggaaaca tttcggccgc gtagcgccac atggcaaaga gtggaagtgg
    12181 atgatggaaa gcgtgctggg tgttcccgcc cgtcgtacgc atcagttcga actgcaatcc
    12241 gtgcgtcgca acaccttccc ctaccgctgc aagtgccagg agcatcagct taccgtacgc
    12301 cgccataatc gcgtagttcg tggcgaggcc gtctatcgct gtgttcactg cggtgaacag
    12361 ctggttgcga aataaccatc tgaactatca ggaactttcc tgatctggct gattgcatac
    12421 caaaacaact ttcgctacgt tgctggctcg ttttaacacg gagtaagtga tgtaccgtta
    12481 tttgtctatt gctgcggtgg tactgagcgc agcattttcc ggcccggctt tggccgaagg
    12541 tatcaatagt ttttctcagg cgaaagccgc ggcggtaaaa gtccacgctg acgcgcccgg
    12601 tacgttttat tgcggatgta aaattaactg gcagggcaaa aaaggcgttg ttgatctgca
    12661 atcgtgcggc tatcaggtgc gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca
    12721 cgtcgttccc gcctggcagt tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa
    12781 aaactgcgct aaagatccgg tctatcgcaa gatggaaagc gatatgcata acctgcaacc
    12841 gtcagtcggc gaggtgaatg gcgatcgcgg caactttatg tacagccagt ggaatggcgg
    12901 cgaaggccag tacggtcaat gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc
    12961 accagcgcgt gcacgcggtg ccattgcgcg cacctacttc tatatgcgcg accaatacaa
    13021 cctgacactc tctcgccagc aaacgcagct gttcaacgca tggaacaaga tgtatccggt
    13081 taccgactgg gagtgcgagc gcgatgaacg catcgcgaag gtgcagggca atcataaccc
    13141 gtatgtgcaa cgcgcttgcc aggcgcgaaa gagctaacct acactagcgg gattcttttt
    13201 gttaacccct accccacgcg tacaaccgcg tggggagacg acgcggattt ttaactatgc
    13261 gtatcccccg catttatcat cctgaaccac tgaccagcca ttctcacatc gcgctttgcg
    13321 aagatgccgc caaccatatc gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat
    13381 tgtttgacgg tagcaaccag gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg
    13441 tggaagtgaa ggtgctggaa ggcaagatcg acgatcgcga atctccgctg catattcacc
    13501 tcggtcaggt gatgtcgcgt ggtgaaaaaa tggaatttac gatccagaaa tcgatcgaac
    13561 tcggtgtaag cctcattacg ccactttttt ctgagcgctg cggcgttaaa ctggatagtg
    13621 aacgtctgaa caagaagctt cagcagtggc agaagattgc aattgctgcc tgtgagcagt
    13681 gtggtcgtaa ccgggtgccg gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag
    13741 agcaggatga aggactgaaa ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt
    13801 tgcctttacc ggttgaacgc gtccgcctgc tgattggccc ggaaggcggt ttatcggcag
    13861 atgaaattgc catgactgcc cgctatcaat ttactgatat cctgttggga cctcgcgttt
    13921 tgcgtacaga gacaactgcg ctcaccgcca ttaccgcgct acaagtacga tttggcgatt
    13981 tgggctaacg gagaagaata atgatcaagc tcggcatcgt gatggacccc atcgcaaaca
    14041 tcaacatcaa gaaagattcc agtttcgcta tgttgctgga agcacagcgt cgtggttacg
    14101 aacttcacta tatggagatg ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata
    14161 cccgcacgct gaacgtgaag cagaactacg aagagtggtt ttcattcgtc ggtgaacagg
    14221 atctgccgct ggccgatctc gatgtgatcc tgatgcgtaa agacccgccg tttgataccg
    14281 agtttatcta cgcgacctat attctggaac gtgccgaaga gaaagggacg ctgatcgtta
    14341 acaagccgca gagcctgcgc gactgtaacg agaaactgtt taccgcctgg ttctctgact
    14401 taacgccaga aacgctggtt acgcgcaata aagcgcagct aaaagcgttc tgggagaaac
    14461 acagcgacat cattcttaag ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga
    14521 aagaaggcga tccaaacctc ggcgtgattg ccgaaaccct gactgagcat ggcactcgct
    14581 actgcatggc gcaaaattac ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg
    14641 tggatggcga gccggtaccg tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg
    14701 gcaatctggc tgccggtggt cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa
    14761 tcgcccgtca gatcgggccg acgctgaaag aaaaagggct gatttttgtt ggtctggata
    14821 tcatcggcga ccgtctgact gaaattaacg tcaccagccc aacctgtatt cgtgagattg
    14881 aagcagagtt tccggtgtcg atcaccggaa tgttaatgga tgccatcgaa gcacgtttac
    14941 agcagcagta acccacctta gcgagaagga tctcgttgag actctgagtg acagcgccct
    15001 tctttccacg catactgggc gctgttgctt ttttgaacca ggaaacagaa cctctgacaa
    15061 tgaatttaca gcatcacttt cttattgcca tgcctgctct ccaggatccg attttccgtc
    15121 gttccgtggt ctacatttgc gaacataata ccaatggtgc aatggggatc atcgtcaaca
    15181 agccgctgga aaatctcaaa attgaaggga ttctggaaaa gctgaagatc acgccggagc
    15241 cgcgtgatga atcaatccgt ctggataaac cggttatgct cggcggtccg ctggctgaag
    15301 atcgcgggtt tattttgcat actccgccct ccaattttgc ttccagcatt cgcatttcag
    15361 acaacacagt gatgaccact tcccgcgatg tgctggaaac gctcggcacc gataaacaac
    15421 cgtctgacgt attggtggct ctgggttatg cctcctggga aaaaggtcaa ctggaacaag
    15481 aaattctcga taacgcgtgg ctaacggccc cggcggatct gaatattctg ttcaaaacac
    15541 cgattgccga ccgctggcgc gaggcggcaa aactgattgg tgtggatatt ctcaccatgc
    15601 ctggtgtggc agggcacgcc tgatgagtgg aaccttactc gccttcgact tcggcaccaa
    15661 aagcattggc gtagcggtcg gccaacgcat taccggcact gctcgccctt tgcctgcaat
    15721 taaagcacag gacggtacgc cggactggaa cctcatcgaa cgtttactga aagagtggca
    15781 gccggacgaa atcatcgtcg gtttgccgct gaatatggac ggcaccgagc aaccattgac
    15841 tgccagagcg cgtaaatttg ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct
    15901 ccatgacgaa cgtcttagca ccgtggaagc ccgttccggt ctgtttgaac agggcggcta
    15961 tcgggcgctc aacaaaggca aagttgactc tgcctctgcg gttattattc tcgaaagcta
    16021 tttcgagcag ggatattaag gcgatttaaa cgcctgctgg agtgtataat atcatcgcgc
    16081 cttacgcctt attgccggat gcggcgtgaa cgccttatcc ggcctacatg tggcagcgat
    16141 tgtaggtctg ataagacgcg ttagcgtcgc atcagacatt tattgccttt ataggcccta
    16201 taaacttgcg catcgggcat ggcaacgtca caaacgccct tcccccaccc gctgctgata
    16261 actctgctga aacgttatca tccccacctg ctgcccggtt tgaataacat gcggtaactg
    16321 gtgggttttc ccttcgcgaa tcaaattccc caccgcggga gtgttaatca ataattcaaa
    16381 cagcgccacg cgtccttcct gtttatccac ttccagcttt tgtgacaaca cggcccgtaa
    16441 actacctgcc agttgattac gtacgggatc tttttcctgc gccggaaatg aatccaccag
    16501 tcgctcaact gcctgcgcgg caccacgcgt atgtaatgtt gccagcacca aatgcccggt
    16561 ttctgccgcc gtcagtgcca gacggattgt ctcgctgtca cgcagctctc cgagcaaaat
    16621 cacatcagga tcttcccgca atgcggcccg caatcccgat gcgaacgtca tacagtgcaa
    16681 accaatttcc cgctgctgga tcaaacatcg ctggctggca tagatatatt caacaggatc
    16741 ttccagcgtc agaatatgcg catcggcatg ttgattgaga tagccaacca tcgccgccag
    16801 cgtggtagat ttgccgctcc ccgtcgcccc cgtcaccaga atcaggccat tctcgctctt
    16861 gagtaattcc ggcaataccg ttggtgcgcc aagctgttcg agctgtggac agtgcgaagg
    16921 taacaaccgt aatgccagag aaataccttg ccgttgcgcg aacgcactgc cacgcaaccg
    16981 ctggttttcc gccagcgaca cggcaaaatc cagctggcca ttttccagca atattgcccg
    17041 ctgatcgtca tccagccact cccgcagtag ctcttcgacg tccggcgcat caaacggcgc
    17101 agcttccatt ctgccacgaa tgcgccatcg tgcgggccag gcgctgcaca ggtgtagatc
    17161 cgagacgtta tgctttacac taagggccac aatttcttcc atattcatac taagatcctc
    17221 ggaaaatgaa cgatattgcg cataacctgg cacaggtccg ggacaaaatc tcagcggctg
    17281 caacgcgttg cggccgttct ccagaagaaa ttacgctgct tgcagtcagt aaaacaaaac
    17341 ctgcgagcgc catcgcagaa gccattgatg ccgggcagcg tcaatttggt gaaaactacg
    17401 ttcaggaagg ggtagataaa attcgccact ttcaggaact gggcgtaaca ggattagaat
    17461 ggcattttat tggcccgttg cagtctaata aaagccgcct ggtggcagag catttcgact
    17521 ggtgtcatac catcgaccgt ttgcgcattg ctacccgcct caacgatcag cgcccggcag
    17581 aacttccccc tcttaacgta ctgattcaaa ttaatattag tgatgaaaac agtaagtccg
    17641 ggattcaact ggcagaactg gacgagctgg cagcggcggt cgctgaacta ccgcgtttac
    17701 gtctgcgcgg gctgatggca atccctgcgc ctgagtcaga atatgtaagg cagtttgaag
    17761 ttgcacgcca aatggctgta gcatttgccg gactgaaaac gcgctacccg catatcgaca
    17821 cgctctctct gggaatgtcg gacgatatgg aagccgccat tgcggcaggt agcacgatgg
    17881 ttcgtatcgg cactgcaatt tttggtgcgc gtgattactc taaaaaataa ggaattaaag
    17941 gaacgccatg aatacgttga ctttcctgct ttcaacggtc attgagctgt ataccatggt
    18001 gctgttatta cgcatctgga tgcagtgggc tcattgtgat ttttacaacc ccttctcaca
    18061 gtttgtagtg aaggtaacac agccaattat cgggccactg cgccgcgtta ttccggcaat
    18121 gggtcccatt gatagcgcct cgctgctggt tgcgtacatt ctcagtttta tcaaagccat
    18181 cgtgctgttt aaagtggtga ccttcctgcc aatcatctgg attgccggtt tactgattct
    18241 gctgaaaacc atcggcctgc tgattttctg ggtcctgctg gtgatggcga ttatgagctg
    18301 ggtaagccag ggtcgtagtc cgattgaata cgtgctgatt cagctggccg atccgctgct
    18361 gcgcccgatt cgccgcctgc taccggcaat gggtgggatt gatttctcgc cgatgatcct
    18421 cgttctgctg ctgtatgtca tcaatatggg tgtcgcagaa gtattacagg caaccggaaa
    18481 catgctgctg ccggggctgt ggatggcgtt atgagtgccg taacagttaa tgatgacggt
    18541 ctggttttac ggctctatat tcagccgaaa gccagccgtg attctattgt cggtttacat
    18601 ggcgacgaag ttaaagtcgc cattaccgcg ccgccggttg acggccaggc caacagccat
    18661 ctggtgaagt ttctcggtaa gcaattccgg gtagccaaaa gccaggtggt aattgaaaaa
    18721 ggcgaacttg gccgccacaa acaaattaaa atcattaatc cgcaacaaat cccgccagaa
    18781 atcgcggcgt taattaatta ggtatcctat gcaaaaagtt gtcctcgcaa ccggcaatgc
    18841 cggtaaagtg cgtgagctgg cgtcgctgct tagcgacttc ggtcttgata tcgtggccca
    18901 aacagacctc ggcgtagatt ccgctgaaga aaccggcctg acctttatcg aaaacgcgat
    18961 tctgaaagcg cgccatgcgg caaaagtgac cggtttaccg gcaattgccg acgactctgg
    19021 tctggcggta gatgcgctgg gtggcgcgcc gggtatttac tccgcgcgtt attccggtga
    19081 agacgcgacc gatcaaaaga atctgcaaaa actgttggaa acactgaaag acgtaccgga
    19141 cgaccaacgt caggcgcgtt tccactgcgt gctggtatat ctgcgtcacg cggaagatcc
    19201 gactccgctg gtgtgccacg gtagctggcc gggcgtgatt actcgtgaac cagcgggcac
    19261 tggtggcttt ggttatgatc caatcttctt cgtaccttcc gaagggaaaa ccgctgccga
    19321 actgacccgc gaagaaaaga gcgccatttc ccaccgtggt caggcattga aactgctgtt
    19381 ggacgcttta cgtaatggtt aaattaccgc cgctgagtct ctacattcac atcccgtggt
    19441 gcgtgcagaa atgcccgtac tgcgatttca actctcacgc gttgaaagga gaagtgccgc
    19501 acgacgatta cgttcagcat ctgctttgcg atctggacaa cgatgtggct tacgctcagg
    19561 gccgtgaagt aaagacaatc tttattggcg gtggtacgcc gagcctgctt tccggcccgg
    19621 cgatgcaaac gctgctggac ggcgtgcgtg cgcgtttgcc gctggcagcg gatgcagaaa
    19681 ttactatgga agcgaaccct ggtacggtag aagccgatcg ctttgtcgat tatcagcgtg
    19741 ctggtgtgaa ccgcatctct attggtgtgc agagttttag cgaagacaag ctgaaacgac
    19801 ttgggcgtat tcatggcccg caagaagcga aacgcgcggc aaagctggcg agcgggctgg
    19861 ggctgcgtag ctttaacctc gatttgatgc atggactacc ggatcaatca ctggaagagg
    19921 cgcttggcga tctacgccag gccattgaac tgaatccgcc gcatctttcc tggtatcaac
    19981 tgaccatcga acctaatacg ctgtttggtt cacgcccgcc ggtactgccg gacgacgacg
    20041 cgctgtggga tatattcgaa caggggcatc agttattaac cgcagcgggt tatcagcaat
    20101 atgaaacttc cgcttacgcc aaacccagtt atcagtgcca gcacaatctc aactactggc
    20161 gctttggcga ctacatcggt attggctgcg gcgcgcacgg caaagtgacc ttcccggatg
    20221 ggcgcattct gcgtaccact aaaacgcgtc atccgcgtgg ttttatgcaa ggaaggtatc
    20281 tggaaagcca gcgtgatgtc gaagccgcag ataagccgtt tgagttcttt atgaatcgct
    20341 tccgtctgct ggaggccgcg ccgcgcgtgg agtttattgc gtataccggg ctttgcgaag
    20401 atgtgattcg cccacagtta gacgaggcga ttgcccaggg ttatctcacc gaatgtgcgg
    20461 attactggca gataacggaa catggtaaat tattcttaaa ctcacttctt gaattattcc
    20521 tcgctgagta atcattaatg cgtctgcggg cgcattaatc acttcttccc cccctcc
//
LOCUS       DAZFYC010000085        20529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFYC010000085 DAZFYC010000000
VERSION     DAZFYC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.085"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHKW5_003442"
     CDS             complement(373..1569)
                     /gene="hemY"
                     /locus_tag="ACHKW5_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO7512778.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1572..2801)
                     /gene="hemX"
                     /locus_tag="ACHKW5_003443"
     CDS             complement(1572..2801)
                     /gene="hemX"
                     /locus_tag="ACHKW5_003443"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO7512779.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFIAIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(2823..3563)
                     /gene="hemD"
                     /locus_tag="ACHKW5_003444"
     CDS             complement(2823..3563)
                     /gene="hemD"
                     /locus_tag="ACHKW5_003444"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO7512780.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3560..4501)
                     /gene="hemC"
                     /locus_tag="ACHKW5_003445"
     CDS             complement(3560..4501)
                     /gene="hemC"
                     /locus_tag="ACHKW5_003445"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO7512781.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4888..7434
                     /gene="cyaA"
                     /locus_tag="ACHKW5_003446"
     CDS             4888..7434
                     /gene="cyaA"
                     /locus_tag="ACHKW5_003446"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO7512782.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7474..7794)
                     /gene="cyaY"
                     /locus_tag="ACHKW5_003447"
     CDS             complement(7474..7794)
                     /gene="cyaY"
                     /locus_tag="ACHKW5_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO7512783.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7842..9167)
                     /locus_tag="ACHKW5_003448"
     CDS             complement(7842..9167)
                     /locus_tag="ACHKW5_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO7512784.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9164..10672)
                     /locus_tag="ACHKW5_003449"
     CDS             complement(9164..10672)
                     /locus_tag="ACHKW5_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO7512785.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11010..11564)
                     /locus_tag="ACHKW5_003450"
     CDS             complement(11010..11564)
                     /locus_tag="ACHKW5_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO7512786.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11914..12117
                     /gene="yifL"
                     /locus_tag="ACHKW5_003451"
     CDS             11914..12117
                     /gene="yifL"
                     /locus_tag="ACHKW5_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7512787.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12154..12978
                     /gene="dapF"
                     /locus_tag="ACHKW5_003452"
     CDS             12154..12978
                     /gene="dapF"
                     /locus_tag="ACHKW5_003452"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO7512788.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12975..13682
                     /gene="yigA"
                     /locus_tag="ACHKW5_003453"
     CDS             12975..13682
                     /gene="yigA"
                     /locus_tag="ACHKW5_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO7512789.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13679..14575
                     /gene="xerC"
                     /locus_tag="ACHKW5_003454"
     CDS             13679..14575
                     /gene="xerC"
                     /locus_tag="ACHKW5_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO7512790.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14575..15291
                     /gene="yigB"
                     /locus_tag="ACHKW5_003455"
     CDS             14575..15291
                     /gene="yigB"
                     /locus_tag="ACHKW5_003455"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO7512791.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15375..17537
                     /gene="uvrD"
                     /locus_tag="ACHKW5_003456"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15375..17537
                     /gene="uvrD"
                     /locus_tag="ACHKW5_003456"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO7512792.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17684..18448)
                     /gene="yigE"
                     /locus_tag="ACHKW5_003457"
     CDS             complement(17684..18448)
                     /gene="yigE"
                     /locus_tag="ACHKW5_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO7512793.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18610..18666
                     /gene="ysgD"
                     /locus_tag="ACHKW5_003458"
     CDS             18610..18666
                     /gene="ysgD"
                     /locus_tag="ACHKW5_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO7512794.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18818..19768
                     /gene="corA"
                     /locus_tag="ACHKW5_003459"
     CDS             18818..19768
                     /gene="corA"
                     /locus_tag="ACHKW5_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO7512795.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19810..20301)
                     /locus_tag="ACHKW5_003460"
     CDS             complement(19810..20301)
                     /locus_tag="ACHKW5_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO7512796.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(20298..>20529)
                     /locus_tag="ACHKW5_003461"
     CDS             complement(20298..>20529)
                     /locus_tag="ACHKW5_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO7512797.1"
                     /translation="QKISLLSFQNYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRF
                     FRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         4833 a         5260 c         5309 g         5127 t
ORIGIN      
        1 ctcaggttaa atattcactc aggaagttat tactcaggaa gcaaagagga ttacagaatt
       61 atctcataac aagtgttaag ggatgttatt tcccgattct ctgtggcata ataaacgggt
      121 agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg aatgacgcag
      181 agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat cagacaccat
      241 ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt aacgcctgct
      301 ccgtaataag agcaggcgtt tttttatgca tcagaaaggt cccggaggtg cttgcctccg
      361 ggtgagaagg aactactgtg gtgggttgtt ctgcaacgtt aacatcaaac cgtcgcgacg
      421 catcgctgca gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa gccatgcgta
      481 atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag gcgagcgatg cttcctgcca
      541 ttctccgtgc ttcatcagtg actggcccag tgtgctccac aacagcgggc gatcgccgac
      601 gtttttgatc tgctggcgca gcactttttc cagctgttcc ggattgttgg ttttcagtcg
      661 aggaatcggc agcagtaggc gatcgtcgta ctggcgtttc aggccatcga tgataatttg
      721 ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca cctgcaacgc
      781 tacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac cttcactacc
      841 gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca gcattgcacg
      901 atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca gcagagaact
      961 ccatgcacct gtgcggatat acgcctgttc cgccagacgt aatacttccg gatggcgtgg
     1021 cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca tggttttcat tacgggccag
     1081 ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca gctctgctgc
     1141 gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg cttcggcagc
     1201 cagtagatag ttcaccaccg gttgttccgc gtgatcggca tttttcgcca tcagcttttc
     1261 aacttgctga taatcgcctt ccgccagttt cagcagcgcc tgttcggtct gcttacgggc
     1321 acggcgacgc ttacgtccga caaaccaccc acgggtgtgt gcgcctgtgc ggaagatccg
     1381 ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaaaa taatcgccag
     1441 gcccgtgacg ctggtttcga tattgtagtt gtcagtctgg atcagcacgt aaccctgatg
     1501 accggcaatc atcgggccaa ccactatccc cgcaatcagc aaaacaaaga gtaataacac
     1561 ttttagcatg gttattctcc ttgcggcgcg gctgccggag tatcaccttg cggtgcaggt
     1621 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct
     1681 tccgttgtcc ccgctgccgg ttgtgctagc aggttacgca cgcgggtctg catcagtttt
     1741 tccagcatcg cctggctttg cagggtttcc ggaagatcca tcgagatatt ttgctggctt
     1801 aactggtcca cctcgtcgag gaacgctttg gtggtggcat catcagtatc gtagtaagct
     1861 cgtacccagg tggagacgtt ctccagcgcc tggcgataag tctcttcctg gtgacgcggt
     1921 acagcttgtg ctgcgaccag caggcgagag cgaatatttt cgcgcagata gatatcctga
     1981 tttggcgcta acagcggtac ggcggtgtca tcacgacggc gaatcgcaat gaagttgtcc
     2041 ataaagttct gccagctttt ttgcagattg atacgccatt cgctgatgga actggaaagc
     2101 tcttcaccgt cggaatccat cggcgaacca tcgctgtcat tatcggccag acgcaggtta
     2161 tctacctgat ttgaaagctg attaagctta aggatgatgc cgtcataatc cacctgcgaa
     2221 actgcagaaa ggctggcgat atcatcggta attgcccgac gaacggtaat cagactcggg
     2281 tcattcatat ccgccaggct ggcatctgca cttttcagca acgctgcagc ggtcgtgacg
     2341 tcctgatcgc tccacagctt ccgtccggcg agtttcacca gaaaatcggc ctgagccagc
     2401 agccaggttt tagcatcgct gccggaaatg gtggcgacct tttgttggac ttcatccaac
     2461 tgttttgcca gcgtttcttg ctgacgattc gcctgcttaa gctgtgcagc ttgttgctta
     2521 ataatgcctt ccagctcggc tttttggctc tcctgggctt tttgcaatgc cgtcagttgg
     2581 ttagccaggg catcgctggt ggcggtctga ttgacggcct gttgtttacc ccagccatac
     2641 aaaccgacac ccgccgccag agcaatagcg atagccaccg cgctgagaat caatgcagta
     2701 ttgttcttac tctttttttc tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg
     2761 gtctcttcaa ccacggcgga ggttttttct tgttccgtca ttatggcttc ctgttatgag
     2821 agttattgta atgcccgtaa aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct
     2881 tgccagccca gttcccgggc gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag
     2941 tgtagtaacc agtgctcacg ataccattgt gggatcagcg accagagttg ctgcaacatt
     3001 tcaccgctgg taacaacgac catcgtcacc tcgcgggatt gccagcgcat cgcttcttct
     3061 gcaccatcgt aatggattgc gcatcgttga taacattcac aaaaagtgac ctcagcaccg
     3121 cgcgctgtca gggtatcccc aattagctca cgaccgccat tgccacgtaa tatcagcgca
     3181 cgtttgcccg caatattttg taattcaggt aattgtagca agacttcgct gatttcccga
     3241 tcctgcgggt agagaatctt ctgtccgctt acggtatgta gcgccagtgc ggtggtgcgt
     3301 ccaatggcga aataatcagg tagtcggggc catttacgat cttgctgatg cagctgtgat
     3361 tgggcaaaag caaccgcgtg ttgcgagagg gcaaacaaca gatcgctctc ccccagcgcc
     3421 gccagttgat cagcaagttg cggtaattgt cgacccggag aaaactcaat cagcggaaaa
     3481 tgccaggcca cctgccccag tgtgcgcaga cggctcacta actcttctcc agcgggagac
     3541 gggcgggtga ccaggatact catgccgggg cgtctccgtt atagacttca gcgaggatct
     3601 cgcgcgcgcc gttattcagt agctcttctg ccagcgaaat ccccatttgt tcggcatttt
     3661 gcggcgcacc gcggcgttca ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg
     3721 cacgcagcca gatttcacca tcaataagct cggcgtagct accaattggc acctgacatc
     3781 caccttcgag acgggtattc atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt
     3841 gattcagcgc ggcaagcagc tcgcgagtgc gcgtatcatc aaggcggcat tcaataccga
     3901 ctgcaccttg tcctaccgcc ggaagagaaa tctcgggtgg taacgcggcg cgaatgcggg
     3961 actccagacc taaacgtttt agtccggcta cggcaagaat gatggcatcg tattcgccgt
     4021 tatctagttt gctcaggcga gtgccgacgt tgccgcgcag ggagcggata atcagatccg
     4081 ggcggcgttc agccagttgg cactggcgac gtaaactgga cgtcccgacg atactgcctg
     4141 ccggtaacgc atccagactg tcataattat tggacacaaa ggcatcgcga ggatcttcac
     4201 gctcacaaat agtgaccaac cccagacctt gcgggaattc aaccggcaca tctttcattg
     4261 aatgtacggc gatatcggcg cgattctcca ggagcgcgac ttccagctct tttacaaata
     4321 agccttttcc gcctactttc gccagcggcg tatcaagaat cacatcgccg cgcgtcacca
     4381 tcggtaccag ttcaaccacc aggcccggat ggctcgccat caacttgtct ttgacatagt
     4441 gtgcctgcca gagtgcaagt gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca
     4501 tgcttgttac cgtcattatc atccgtggtc catcctaaca tccttgccag agtgatgtca
     4561 gtgttgtggt gaaacgtaga cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc
     4621 tgacaggcgt ttcacgccgt cgtaataagg aatttacaga gaataaacgg tgctacactt
     4681 gtatgtagcg catctttctt tacggtcaat cagcaaggtg ttaaattgat cacgttttag
     4741 accatttttt cgtcgtgaaa ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt
     4801 gacatacgaa atatcccgaa tgccgcgtgt taccgttgat gttggcggaa tcacagtcat
     4861 gacgggtagc aaatcaggcg atacgtcttg tacctctata ttgagactct gaaacagaga
     4921 ctggatgcca taaatcaatt gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc
     4981 caacaggtct acagtctact gccgacattg ttgcactatc accatccgct aatgccgggt
     5041 taccttgatg gtaacgttcc caaaggcatt tgcctttaca cgcctgatga aactcaacgc
     5101 cactacctga acgagcttga actgtatcgt ggaatgtcag tacaggaccc accgaaaggt
     5161 gagcttccaa ttactggtgt atacaccatg ggcagcacct cgtccgtagg gcaaagttgt
     5221 tcctctgacc tggatatctg ggtctgtcat caatcctggc tcgatagcga agagcgccag
     5281 ttactacaac gtaaatgtag tctgctggaa agctgggccg cctcgctggg tgtggaagtc
     5341 agcttcttcc tgattgatga aaaccgcttc cgtcataatg aaagcggcag cctggggggc
     5401 gaagattgtg gctccaccca gcatatactg ctgcttgacg aattttatcg taccgccgtg
     5461 cgtctcgccg gtaagcgtat tctgtggaat atggtgccgt gcgacgaaga agagcattac
     5521 gacgactatg tgatgacgct ttacgcgcag ggcgtgctga cgccaaatga atggctggat
     5581 ctcggtggct taagctcgct ttctgctgaa gagtactttg gtgccagcct ttggcagctc
     5641 tacaagagta tcgattcccc atacaaagcg gtactgaaaa cactgctgct ggaagcctat
     5701 tcctgggaat acccgaaccc acgtctgctg gcgaaagata tcaaacagcg tttgcacgac
     5761 ggcgagattg tatcgtttgg tctcgatcca tactgcatga tgctggagcg tgttactgaa
     5821 tacctgacgg cgattgaaga ttttacccgt ctggatttag tacgtcgctg cttctattta
     5881 aaagtgtgcg aaaagctcag ccgtgaacgc gcctgcgtag gctggcgtcg cgcagtgttg
     5941 agccagttag tgagcgagtg gggttgggac gaagctcgtc tggcaatgct cgataaccgc
     6001 gctaactgga agattgatca ggtgcgtgag gcgcacaacg agttgctcga cgcgatgatg
     6061 cagagctacc gtaatctgat ccgctttgcg cgtcgcaata accttagcgt ctccgccagt
     6121 ccgcaggata tcggcgtgct gacgcgtaag ctgtatgccg cgtttgaagc attaccaggt
     6181 aaagtgacgc tggtaaaccc gcagatttca cccgatctct cggaaccgaa tctgaccttt
     6241 atttatgtgc cgccgggccg tgctaaccgt tcaggttggt atctgtataa ccgcgcgcca
     6301 aatattgagt cgatcatcag ccatcagccg ctggaatata accgttacct gaacaaactg
     6361 gtggcgtggg catggtttaa cggcctgctg acctcgcgca cccgtttgta tattaaaggt
     6421 aacggcattg tcgatttgcc taagttgcag gagatggtcg ccgacgtgtc gcaccatttc
     6481 ccgctgcgct tacctgcacc gacaccgaag gcgctctaca gcccgtgtga gatccgccat
     6541 ctggcgatta tcgttaacct ggaatatgac ccgacagcgg cgttccgcaa tcaggtggtg
     6601 cattttgatt tccgtaagct ggatgtcttc agctttggcg agaatcaaaa ttgcctggta
     6661 ggtagcgttg acctgctgta ccgcaactcg tggaacgaag tgcgtacgct gcacttcaac
     6721 ggcgagcaat cgatgatcga agccctgaaa actattctcg gcaaaatgca tcaggacgcc
     6781 gcaccgccag atagcgtgga agtcttctgt tatagccagc atctgcgcgg gttaatccgt
     6841 actcgcgtgc agcaactggt ttctgagtgt attgaactgc gcctttccag cacccgccag
     6901 gaaaccgggc gtttcaaggc gctgcgcgtt tctggtcaaa cctgggggtt gttcttcgaa
     6961 cgcctgaatg tatcggtaca aaaactggaa aacgccatcg agttttatgg cgctatttcg
     7021 cataacaaac tgcacggctt gtcagtgcag gttgaaacca atcacgtcaa attaccggcg
     7081 gtggtggacg gctttgctag tgaagggatc atccagttct tcttcgaaga aacgcaagac
     7141 gagaatggct ttaatatcta cattctcgac gaaagcaacc gggttgaggt gtatcaccac
     7201 tgcgaaggca gcaaagagga actggtgcgt gacgtcagtc gcttctactc gtcatcgcac
     7261 gaccgcttca cctacggctc aagcttcatc aacttcaacc tgccgcagtt ctatcagatt
     7321 gtgaaggttg atggtcgtga acaggtgatt ccgttccgca caaaatctat cggtaacatg
     7381 ccgcctgcca atcaggatca cgatacgccg ctattacagc aatatttttc gtgatgaacg
     7441 tgccggaaag cgaggcttat ccggcatgca atcttagcgg aaactgactg tttcacccgc
     7501 ttgctgtgtc gccgcctgtt ccagcaaatc ccagaaggtt tcgccgctgc gatcgcaaat
     7561 ccattcatcg ccttttaggt caaaatggta gccgccctgt ttggttgcca gccacacctg
     7621 gtgcagcggc tcctggcggt taataatgat tttgctgccg ttttcaaagg taatggtcag
     7681 tacgccgccg ttgatttcgc agtcgatatc gctgtcgcca tcccagtcgt ccaggcgttc
     7741 ttcaatggtc agccagagtt gatcagccag gcgatgaaat tcactgtcgt tcattgttgt
     7801 atcctgtctt taagtgatgg cggcagtata gcggtatggg gtcagggctt caaagtttgc
     7861 ccgtctgttg ctgcgttccg gcatgagtca tccgtcaacg gaataataat gtctctgttg
     7921 tgaagttcac gcacattctc ttctctgacg tgtgttttcg ccgggcaggg ggagggcgta
     7981 atccttacct ctgtatcggt ccatgtataa acattgcgat cgtcataagc gaagcgatcg
     8041 tttttagtta gcggaccgtt aactggaata atttgcacgc tctttgaccg taattggcgg
     8101 ataaaataag catgctgttt gccgctatta aatgagcgat caatgaccag gtctggcgta
     8161 tccagcggga ttatctcaag ctggtcttca ccaaatgtcg ataattttgt tacatacaac
     8221 cgatctttat attgcgcaat attgccagta atcagatgga attgttcagg ttcaatatct
     8281 ggtaggtcta tccacttgct ccactgacgg tcggacgtga gctgtttacg ccagcgaacg
     8341 cgactttccc ctacttcaaa agagacgtca tcatctacgg gtatcgcttt gccagacaat
     8401 tgcccaaagg gataacgatg cataccctta ttgtcgcgaa aaaccagcag tgaatgaggt
     8461 atccagcgaa ttatctcaaa tgagtctgca ttcgccgtaa ggtgaacgcc atttaaataa
     8521 atattctgcc cactgcggat aagcagatca ttggaataca aatttctgtt tacactcata
     8581 agacgtggat taatacccca ccatcttttt tctaatacag taacattact gctgctcccc
     8641 tgacgtcgac cggacaaata gagattttta ctatccacca gcgtggtaga gttaccttcg
     8701 atagctttta gtgtcgccag atcaacacgt gacgcgccgc tattactttc ggtgcgctgt
     8761 ccgtcaaaat agaggctata tttatcgacc gcgaagcggc ccatagcctg aaagctggca
     8821 atatcgacag tgggtgttcc cggtggattg ctaacgattt tgccacgcca gagaataacg
     8881 cggccgtctg tcacccagtg gtattcctca ttttttatgc ttatttcatc ggtgggtata
     8941 tagccttcca ccaactcccg ctccccggat atatttgtca caaggcgcaa atcggctctg
     9001 ttcatcttcg gaaattgggc aatgattttc ttatcccccc aataaccttt accgtaagac
     9061 ataacaccgt gatcattgta atagtcgaac cctatggcct gtacagcagg cgtcagaaat
     9121 gtgtgtccca acaggatgag tgcaactgtg attatttgac ccttcatttt gtcgcctcat
     9181 tgcgcggggc aggaataaat tcgccgtcat cagtaatttt tccgtccaga tatcccgttt
     9241 tgtaatttac gccatagcca tatcccgaat gttgttcctt ccagattaag cgaacttgtt
     9301 tcggatcagc ggtgcggtat cgccagagct gcgcgccatc aaagctgtaa atgtagcggt
     9361 tatcgcgggc ataccatctg cgcaaaaagg gatggtcatt aagatgggct tcgcgttcat
     9421 ccggagaacg gttatccagt aagatcagcg gtccgtcaga ttcaaacaca tgtaaaccgg
     9481 aatggtatgg ccacccttcg gcacgcgtaa gtaaatagcc gtggcgtttc ccggcgttga
     9541 aacgtttgtt gataataaga ttgggatcat caagtttcac gatttccagc ttgcggtcac
     9601 cagactctgt ttcgatgatg gtataaagcg agtcctgata ctgggcgaca gcatcactga
     9661 tgggttgaaa ctgctccgga tcgagtccgg gtaattcttc ctgtttacaa gcaggtcctt
     9721 tacgccagaa cactttcttt tccagcagag aaaaatccag acagtgcttt gttaaatctt
     9781 catcccactc cagattttcc ttgtcgagga ctttacgctg cagcccgttt ttatcacgcc
     9841 aggtcaagag tgagccgggc atccagcgca cgacggaaaa ggtgtccggg tcggcatcaa
     9901 gctgttcgcc gttgatcagg attttggtcc gtgtacgagc cagggtatcc caggggccaa
     9961 atggcatagc cacacaggga ttaaacgcgg tagaaaactt tcctcgctga tcccatgact
    10021 tttgtgccag cacacggaaa ctatcgggat ctgcgaggcg atgcccattg atatacaaga
    10081 aattagcgtc acgtaatatc agccccagta agtcaggatc ccagggaggc cggaattcca
    10141 cctgatgcag tgttttgata tctaagcgat ttcccccgcc attgttatcg gtgcgcttgc
    10201 cttcgaaata gagactgtgt ttatctgcgg caaaatcacc ccacgccttg aacgaggcga
    10261 catcgacggg aggtttgtct ggcgggtttt gcacaacttc acccgcgtag aggatatagc
    10321 gaccatcgct atgccaggag taatgaggat accaggcctg tgcgccttca tagaggtagt
    10381 cggttgtcag atcagtcaga ggaggtactt tatcccattt gtctagtgtt gtcgggtcta
    10441 ttaataaatt gggtaaacgg cgcaaacttt tgtaattcac gtcaggaaga gaaagatttg
    10501 tttctttata agggcgcagg tataccacgc cttttccctg tttctggtaa atattatgtt
    10561 caggcggatc aagccgacat ccccatgccg acgataaaaa caatgaacaa gacaacaaac
    10621 aaaccacatt gcgatagtgc ataaagccat cctggcgcga ggtgccgatc acgaaactac
    10681 cagcagaaca taattcctcg cacgtaagcg taacttttgc ggcaaatcgt cacttttcag
    10741 aatctataaa ggccatcgct attttctgat agttgctggg gtaaacttga ggttttcatt
    10801 cacgatataa atacgagcta ctgcgccatg gtgtgactga tcacgttaaa atgtgccgga
    10861 ttgacgccgg gaagggtgtc aacatgcgac tctgcccacc gttacctact ttattgtctt
    10921 tcgcgtgttg tacttaggtc ccaccaccgg tatctaacat tccccacatt attacgcttt
    10981 ctgccgaacg tgatttttat agaaatctat caattcattt tccctgtacc ataaagttta
    11041 tctttcacat cctgccgaac gcgggtcttg taccccccgt tgtgaagatc ttcatacgga
    11101 gaaaaagaat ctttactcca ccccagcagc actttatcgc caggcataaa atgcacatgc
    11161 agaaaatctt ctccccattc tcttttgggg agaggtaacg tcatattccg tttctccaga
    11221 cgcattccag cattatactg tgtacgagta gtacttaatg tccagataac ctctgccgta
    11281 tccccactaa catcaccaca acaggttgtt gaaccgccac catttgcaaa agcattcgcg
    11341 cccgccacgc cgttaacact gaatcccagt atcgggcggt caatttcact gtggatttcc
    11401 agaatcactc cacctcttgg tcctccccat actgcctgtc ctatattcca caacacgacc
    11461 agtaccggaa cgacaattaa cgtccgccat acccagcgcc cccaccgggc ataaccgcgc
    11521 gtaagctttt ggtcaaattt ttccagttgg gtaaatagcc ccatcagatt ttattccctc
    11581 ttatcaccag gttggcaaag ttgtatgtca ctggcgacta aggtcagaca acgaaattgt
    11641 atggtgcgcc actctattaa aagccccatg ccgacgataa gaacaatgaa caagacaaca
    11701 aacaaaccac attgcgatag tgcataaagc catcctggcg cgaggtgccg atcacgaaac
    11761 taccagcaaa acataaatcc ccacgtgtaa gcgttatact cgcagcattt cctcactttt
    11821 cagacttcat aaagagtcgc taaacgcttg cttttacgcc ttctcctgcg atgatagaaa
    11881 gcagaaagcg atgaacttta caggcaatcc ataatgaaaa acgtgtttaa ggcactcact
    11941 gtattaatta ctctcttcag cctgacgggc tgcggtctga aaggtccgct ctatttcccg
    12001 cctgcagata aaaacgcacc gccgccgacc aaaccggtag agacgcaaac gcaatccacg
    12061 gtgccggata aaaacgatcg cgccactggc gatggtccat cccaggtgaa ttactaaaag
    12121 tcagtttctg tacccgcgtg attggagtaa atgatgcagt tctcgaaaat gcatggcctt
    12181 ggcaacgatt ttatggtcgt cgacgcggta acgcagaatg tctttttttc accggagctg
    12241 attcgtcgcc tggctgatcg gcacctggga gtagggtttg accaactgct ggtggttgag
    12301 ccgccgtatg atcctgaact ggattttcac tatcgcattt tcaatgctga tggcagtgaa
    12361 gtggcgcagt gcggcaatgg tgcgcgctgc tttgcccgtt ttgtgcgtct gaaagggctg
    12421 accaataagc gcgatatccg cgtcagcacc gccaacgggc ggatggttct gaccgtcacc
    12481 gatgatgatc tggtccgcgt aaatatgggc gaacccaact tcgaaccttc cgccgtgccg
    12541 tttcgcgcta acaaagcgga aaagacctat attatgcgcg ccgccgagca gacaatctta
    12601 tgcggcgtgg tgtcgatggg aaatccgcat tgcgtgattc aggtcgatga tgtcgatacc
    12661 gcggcggtag aaacgcttgg tcctgttctg gaaagccacg agcgttttcc ggagcgagcc
    12721 aatatcggtt ttatgcaagt ggttaagcgc gagcatattc gtttacgcgt ttatgagcgt
    12781 ggggcaggag aaacccaggc ctgcggcagc ggcgcatgtg cggcggtggc ggtagggatt
    12841 cagcaaggtt tgctggccga agaagtacgc gtggaactcc ccggcggtcg tcttgatatc
    12901 gcctggaaag gtccgggtca cccgttatat atgactggcc cggcggtaca tgtctacgac
    12961 ggatttattc atctatgaag caaccagggg aagaactgca ggaaacactc acggagcttg
    13021 atgaccgggc ggttgtcgat tatctgatta aaaatcctga gttttttatc cgtaatgcgc
    13081 gcgcagtaga agcgatacgt gtgccgcatc cggtacgcgg caccgtttcg ttggtcgagt
    13141 ggcacatggc ccgcgcacgt aatcatattc atgttctgga agagaacatg gcgctgttga
    13201 tggaacaggc tatcgccaac gaaggcctgt tttatcgcct actctacctg cagcgcagtc
    13261 tcaccgccgc cagcagtctc gacgatatgc tgatgcgctt tcaccgctgg gcgcgcgatc
    13321 tcggcctggc aggtgcgagt ctgcgcctgt ttccggatcg ctggcgctta ggtgcgccgt
    13381 cgaaccacac tcatctggca ttaagccgtc agtctttcga accgctgcgt attcagcgtt
    13441 tggggcagga acagcactat cttgggccgc ttaacggacc agagttgctg gtggtgctac
    13501 cggaagcgaa agcggtggga tcggtggcga tgtcgatgct gggaagcgat gctgatttgg
    13561 gtgttgtgct gtttaccagt cgcgatgcca gtcactatca acaagggcaa ggaacgcagt
    13621 tacttcatga aattgcgctg atgttgccgg agcttctgga gcgttggatt gaacgcgtat
    13681 gaccgattta cacaccgatg tagaacgcta cctacgttat ctgagcgtgg agcgccagct
    13741 tagcccgata accctgctta actaccagcg tcagcttgag gcgatcatca attttgccag
    13801 cgaaaacggc ctgcaaagct ggcagcaatg tgatgtgacg atggtgcgca attttgctgt
    13861 acgcagtcgc cgtaaagggc tgggagcagc aagtctggcg ttacggcttt ctgcgctacg
    13921 tagctttttt gactggctgg tcagccagaa cgaactcaaa gctaacccgg cgaaaggagt
    13981 ttcggcaccg aaagcgccgc gtcatctgcc gaaaaacatc gacgtcgacg atatgaatcg
    14041 gctgctggat attgatatca acgatcccct cgctgtacgc gaccgtgcaa tgctggaagt
    14101 gatgtacggc gcgggtctgc gtctttccga gctggtgggg ctggatatca aacacctcga
    14161 cctggagtct ggtgaagtgt gggtaatggg gaaaggcagc aaagagcgcc gcctgccgat
    14221 tggtcgcaac gctgtggcgt ggattgagca ctggcttgat ttgcgcgacc tgtttggtag
    14281 cgaagacgac gcgctttttc tgtcgaaact gggcaagcgt atctccgcgc gtaatgtgca
    14341 gaaacgcttt gccgaatggg gcataaaaca agggctgaat aatcacgttc acccacataa
    14401 attacgtcac tcgttcgcta cgcatatgct ggagtcgagc ggcgatcttc gtggtgtgca
    14461 ggagctgctg ggtcatgcca acctctccac cacgcaaatc tatactcatc ttgattttca
    14521 acaccttgcc tcggtgtacg atgcggcgca tccacgcgcc aaacggggga aataatgcgt
    14581 ttttaccggc ctttggggcg catctcggcg ctcacctttg acctggatga taccctttac
    14641 gataaccgtc cggtgatttt gcgcaccgag cgagaggcgc ttacctttgt gcaaaattat
    14701 catccggcgc tgcgcagctt ccagaatgaa gatctgcaac gcctgcgcca ggcggtacgg
    14761 gaagcggaac ccgagattta tcacgacgtg acgcgttggc gttttcgttc gattgaacaa
    14821 gcgatgctcg acgccgggct gagtgccgaa gaagccagtg caggcgcaca cgcagcaatg
    14881 attaactttg ccaaatggcg cagccgaatc gacgtcccgc agcaaactca cgacacctta
    14941 aaacagctgg cgaagaaatg gccgctggtg gcgatcacca acggtaacgc ccagccggaa
    15001 ctgtttggtt tgggggatta ttttgagttt gtgctgcgcg ctggcccgca cgggcgctca
    15061 aaaccgttca gcgatatgta ctttttggct gcggaaaaac tcaacgtgcc gatcggcgag
    15121 atcttacatg ttggggacga tctcaccact gacgtgggtg gggcaattcg cagcggaatg
    15181 caggcttgtt ggatcagacc ggaaaatggc gatctgatgc aaacctggga cagccgttta
    15241 ctgccgcatc tggaaatttc ccggttggca tctctgacct cgctgatata atcagcaaat
    15301 ctgtatatat acccagcttt ttggcggagg gcgttgcgct tctccgccca acctattttt
    15361 acgcggcggt gccaatggac gtttcttacc tgctcgacag ccttaatgac aaacagcgcg
    15421 aagcggtggc cgcgccacgc agcaaccttc tggtgctggc gggcgcgggc agtggtaaga
    15481 cgcgcgtact ggtgcatcgt atcgcctggc tgatgagcgt ggaaaactgc tcgccatact
    15541 cgattatggc ggtgacgttt accaacaaag cggcggcgga gatgcgtcat cgtatcgggc
    15601 aactgatggg caccagccag ggcggcatgt gggtcggcac cttccacggg ctggcgcacc
    15661 gcctgctgcg tgcgcaccat atggacgcca atctgccgca ggatttccag atcctcgata
    15721 gcgaggacca gctgcgcctg cttaagcgtc tgatcaaagc catgaacctc gacgagaagc
    15781 agtggccgcc gcgccaggca atgtggtaca tcaacagcca gaaagatgaa ggcctgcgtc
    15841 cgcatcatat tcaaagctac ggtaatccgg tggagcagac ctggcagaag gtgtatcagg
    15901 cgtatcagga agcgtgtgac cgcgcgggcc tggtggactt cgccgagctg ctgctgcgcg
    15961 ctcacgaact gtggcttaac aagccgcata tcctacaaca ctaccgcgaa cgttttacca
    16021 atatcctggt ggacgaattc caggatacca acaacattca gtacgcgtgg atccgcctgc
    16081 tggcgggcga caccggcaaa gtgatgatcg tcggtgatga cgaccagtca atctacggct
    16141 ggcgcggggc gcaggtggag aatattcagc gtttccttaa tgatttcccc ggtgccgaaa
    16201 ctattcgtct ggagcaaaac taccgctcta ccagcaatat tctgagcgcc gctaacgccc
    16261 tgattgaaaa caataacggg cgtctgggta aaaaactgtg gaccgatggc gcggacggtg
    16321 agcctatttc cctctattgc gcttttaacg aactcgatga agcgcgtttt gtggttaacc
    16381 gcatcaaaac ctggcaggac aacggcgggg cgcttgccga gtgcgccatt ctctaccgca
    16441 gtaacgccca gtcgcgtgta ctggaagaag cactgttgca ggccagtatg ccgtaccgta
    16501 tttacggcgg gatgcgcttc ttcgaacgcc aggaaatcaa agacgcgctc tcgtatctgc
    16561 gtctgattgc caaccgtaac gacgacgcgg cgtttgagcg cgtggtgaat acgccaacgc
    16621 ggggtattgg tgaccggacg ctggacgtgg tacgtcagac atcgcgcgat cgccagttaa
    16681 cactctggca ggcatgtcgt gaactgttgc aggaaaaagc cctcgccggg cgagctgcca
    16741 gcgccttgca gcgatttatg gaattaatcg atgccttagc gcaagaaact gccgatatgc
    16801 cgctgcatgt acagactgac cgggtaatta aagactccgg cctgcgcacc atgtacgagc
    16861 aggagaaggg cgaaaaaggc cagacgcgta tcgaaaactt agaggaactg gtgacggcaa
    16921 cgcgccagtt cagctacaac gaagaagatg aagatttaat gccgctgcag gcattcctct
    16981 cccatgcggc gctggaagcg ggcgaggggc aggcggatac ctggcaggat gcagtgcagt
    17041 tgatgacgct gcactcggcg aaaggcctgg agttcccgca ggtgtttatt gtcggtatgg
    17101 aagagggtat gttcccaagc cagatgtcgc tggatgaagg cgggcgtctg gaagaagaac
    17161 gccgtctggc ctacgttggc gtaacccgcg cgatgcagaa actgacgctg acctacgcgg
    17221 aaactcgccg tctgtatggc aaagaggttt accatcgccc gtcgcgcttt attggcgagc
    17281 tgccggaaga gtgtgtggaa gaggtgcgcc tgcgcgccac ggtaagccgc ccggtcagcc
    17341 atcagcggat gggtacgccg atggtcgaga acgacagcgg ctacaagctc ggccagcgcg
    17401 tacgccacgc taagtttggt gaaggcacca ttgtcaatat ggaaggcagc ggtgagcata
    17461 gccgtttgca ggtggcattt cagggccagg gtattaaatg gctggtggcg gcttatgccc
    17521 ggctggagtc ggtgtaacgt tgccggatgc ggtgctgcgc accttatttg gcctaaaaaa
    17581 tcattcagat tcaataaatt gcaacgtcat gtaggccgga taaggcgttt acgccgcatc
    17641 cggcatcagc agcatattta gtatctgaca caaaactatc attctaacct ttccgctcga
    17701 cggaaatcat ggtgacaaaa ggatagcgtt gccacgggat tgccccgcct ttcatataca
    17761 tatgtgaaat cgtgccatca agataaagca gctgctcaac gttcagtttc gctttggcat
    17821 aacaggcaaa atcataaaaa tttgtcgcct gctggctcaa caaaaacacg gcattcccat
    17881 gtttattaat cccaacaccg ttacgaattt tgcgtgaggc gacgttggga tgaatacgcg
    17941 gattaattac accgttttcc atcaacattg gccctgactg caccgcaaac tgaatctctt
    18001 tactggtttt gaaggcatcc agacgaacga tgccgacttt atctcccgcg acataaaaca
    18061 cgccgccagg acggataaag aaattccctt cacctgaagc gagatttaac gccaccttct
    18121 gctgaccgtt ttcgatgtac aaaccgagcg gcgcatagct ttcatcatag atgccgccgt
    18181 tcatcgccat ctgcacctga ccctgactat tcatatccgc cagcagagca tgtaacgttc
    18241 cccacgcttc gccattagct ttttgccagt acattttcac ccgctctgtt tgaggattaa
    18301 cggtatacgc ctgtacggtc agcgtcggat ctgagagtgc gcaatcatca gcggcaacag
    18361 caaacaaggg aagtaaggtg agggtgagaa aaatccgttt gagattcaag gtgatcattc
    18421 ctttaccaat gagtagccga tgcgccatta taggtcctgg atgttggatt tttttatcct
    18481 gttagcgacc ttgacgagta ccaaaaagcg cgaagttcaa ctattgttct gtggtgttct
    18541 gttgcgtgtt gacggcaaaa ttttgctggc gtaacatgcg cgcacgatca ctctaagagg
    18601 acattcgcct tggacacacc cagtagatac tggctcacta tcctgtcatc caggatcaac
    18661 tcctaaggct atcccttttt gctgatagcc ttagcggttg tcagcgacct caatttttcc
    18721 cgtcgcgctg agtcaggctg tttaatggtc tgaaacccaa tttgtttctg tgtgcccacc
    18781 gaactgtccg atattttaag cattgggagt cccggtcatg ctgagcgcat ttcaactgga
    18841 aaataaccga ctgacccggc tggaagtcga agagtcacaa ccccttgtaa atgcagtatg
    18901 gattgatctt gtcgaaccgg acgacgacga gcgactgcgc gtacaatctg aacttggcca
    18961 gagcctggca acccgcccgg aactggaaga catcgaagca tcggcacgtt tctttgaaga
    19021 cgacgacggc ctgcatattc actccttctt cttctttgaa gatgcggaag atcacgccgg
    19081 taactccact gtggcattta ccatccgtga tggtcgtctg tttactctgc gtgagcgtga
    19141 actgcccgct tttcgtctgt atcgtatgcg tgcccgtagc cagtcgatgg tagacggtaa
    19201 cgcctacgag ttgctgctgg atctgttcga aaccaaaatc gaacagttgg cagatgaaat
    19261 tgaaaatatc tatagcgacc tggagcagtt gagccgggtg attatggaag ggcatcaggg
    19321 cgatgagtac gacgaggcgc tctccacgct ggcggaactg gaagatatcg gctggaaagt
    19381 tcgcctgtgt ctgatggata cccagcgcgc gctcaacttc ctggtacgca aggcgcgttt
    19441 accgggtggg caactggagc aggcgcgtga aatcctgcga gatatcgaat ccctgctgcc
    19501 gcataacgaa tccctgttcc agaaggtgaa cttcctgatg caggcggcga tgggctttat
    19561 caacatcgag cagaaccgca tcatcaaaat cttctcggtg gtatccgtgg tgttcctgcc
    19621 accgacgctg gtggcttcca gctacgggat gaactttgaa tttatgccag agttgaagtg
    19681 gagtttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg caccgtatct
    19741 gtactttaag cggaagaact ggttgtaata tgagtgccgg ataaagctat ttttttatcc
    19801 ggcttatttc taaaaattgt cttttttaaa gtcatgcgga ttgaaaactc gctctatttt
    19861 attttcttgt gttaattgat actcagtaaa atcgttaata ccaatatgag tgttacgata
    19921 agggtattca ttaataacca ttaagtaatt tttcacgatg caattatcat tgctcttaat
    19981 tgtcgggaaa cagtagagat cttcatacgg cccttcgata tattttttat agtcagtgaa
    20041 gccattaccg atatcaatga cataccaggt atgccaacta ccttctggta ggggatgttg
    20101 atttttaatt tcattctgat gcttcagata ccaatttatt ttatcgctgt cagtccatgg
    20161 cagatgatca acgaccagaa agactgaatt gccagcatca gagtatgtat acaaaatctg
    20221 tacaggacgc aatgtccata aattatagat gcagcataga agaaataaac ataatataat
    20281 tttctggagt ttgcttttca tagataattt tcgattcttt cgatagtgtt aaaattggtg
    20341 aaaaaaggtt tgaaagcaaa gtctttatgc cgttgtaaaa agaaccatat gcgaaaaaat
    20401 ctatatttgc tgtaagtctt gtttttgata tcagttacat caagaccgaa gtggtcctgt
    20461 gctacaaaat gaattgttgc actccatcct atagcgtagt tttgaaaact taaaagagat
    20521 attttttga
//
LOCUS       DAZFYC010000086        19994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFYC010000086 DAZFYC010000000
VERSION     DAZFYC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.086"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..226
                     /locus_tag="ACHKW5_003462"
     CDS             5..226
                     /locus_tag="ACHKW5_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7512798.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAHTCREVQ
                     DIPGVLAVFAERRKDSFGLYVRLMSVTLN"
     gene            389..757
                     /locus_tag="ACHKW5_003463"
     CDS             389..757
                     /locus_tag="ACHKW5_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7512799.1"
                     /translation="MSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGVCLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            847..1173
                     /locus_tag="ACHKW5_003464"
     CDS             847..1173
                     /locus_tag="ACHKW5_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7512800.1"
                     /translation="MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDN"
     gene            1221..1709
                     /locus_tag="ACHKW5_003465"
     CDS             1221..1709
                     /locus_tag="ACHKW5_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7512801.1"
                     /translation="MKLALTPEADSVNVQALNMGRIVVDVDGIELAELINVVCDNGHS
                     LRVVDESDRASTDCTPSFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRQGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            1721..1918
                     /locus_tag="ACHKW5_003466"
     CDS             1721..1918
                     /locus_tag="ACHKW5_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7512802.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLQQNR"
     gene            2003..2845
                     /locus_tag="ACHKW5_003467"
     CDS             2003..2845
                     /locus_tag="ACHKW5_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7512803.1"
                     /translation="MSDITISRPELVNRHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISAMTESIGGKTAPAWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQAHDTWYQSLEYDNFPEISEANILSTFEQLHQNKDEVFERGMINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQCKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPG
                     ALAM"
     gene            complement(3188..3724)
                     /gene="yghD"
                     /locus_tag="ACHKW5_003468"
     CDS             complement(3188..3724)
                     /gene="yghD"
                     /locus_tag="ACHKW5_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO7512804.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIADRGENIQVWIDPVVFNDLLKWLNALDEKYALRVTQIDAS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(3726..4904)
                     /gene="gspL"
                     /locus_tag="ACHKW5_003469"
     CDS             complement(3726..4904)
                     /gene="gspL"
                     /locus_tag="ACHKW5_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7512805.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMACDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGKP
                     EGNLLTGPWQPRVSYRKQWARWRVMILQILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(4901..5878)
                     /gene="gspK"
                     /locus_tag="ACHKW5_003470"
     CDS             complement(4901..5878)
                     /gene="gspK"
                     /locus_tag="ACHKW5_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7512806.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNETELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5881..6480)
                     /gene="gspJ"
                     /locus_tag="ACHKW5_003471"
     CDS             complement(5881..6480)
                     /gene="gspJ"
                     /locus_tag="ACHKW5_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7512807.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6477..6848)
                     /gene="gspI"
                     /locus_tag="ACHKW5_003472"
     CDS             complement(6477..6848)
                     /gene="gspI"
                     /locus_tag="ACHKW5_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7512808.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(6845..7408)
                     /gene="gspH"
                     /locus_tag="ACHKW5_003473"
     CDS             complement(6845..7408)
                     /gene="gspH"
                     /locus_tag="ACHKW5_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7512809.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(7412..7867)
                     /gene="gspG"
                     /locus_tag="ACHKW5_003474"
     CDS             complement(7412..7867)
                     /gene="gspG"
                     /locus_tag="ACHKW5_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7512810.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7884..9107)
                     /gene="gspF"
                     /locus_tag="ACHKW5_003475"
     CDS             complement(7884..9107)
                     /gene="gspF"
                     /locus_tag="ACHKW5_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7512811.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAVGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPAMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFSP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9107..10600)
                     /gene="gspE"
                     /locus_tag="ACHKW5_003476"
     CDS             complement(9107..10600)
                     /gene="gspE"
                     /locus_tag="ACHKW5_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7512812.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(10600..12660)
                     /gene="gspD"
                     /locus_tag="ACHKW5_003477"
                     /pseudo
     CDS             complement(10600..12660)
                     /gene="gspD"
                     /locus_tag="ACHKW5_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12690..13649)
                     /gene="gspC"
                     /locus_tag="ACHKW5_003478"
     CDS             complement(12690..13649)
                     /gene="gspC"
                     /locus_tag="ACHKW5_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7512813.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(13667..14077)
                     /gene="gspS2"
                     /locus_tag="ACHKW5_003479"
     CDS             complement(13667..14077)
                     /gene="gspS2"
                     /locus_tag="ACHKW5_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO7512814.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(14143..14952)
                     /gene="pppA"
                     /locus_tag="ACHKW5_003480"
     CDS             complement(14143..14952)
                     /gene="pppA"
                     /locus_tag="ACHKW5_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO7512815.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAIITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(15100..19662)
                     /gene="sslE"
                     /locus_tag="ACHKW5_003481"
     CDS             complement(15100..19662)
                     /gene="sslE"
                     /locus_tag="ACHKW5_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO7512816.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRV
                     TGDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDA
                     QELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFK
                     KLVNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIIL
                     SEGRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKS
                     TIALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLS
                     NGATLDEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQ
                     IQGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARND
                     KNYWLAFGEKRAWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVI
                     GNPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKS
                     SMTVGTNLDTVYFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNG
                     FEYVTQWGSDPYSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESA
                     SGFVRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTID
                     DTTKEVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQ
                     RILDAFPGLEVCKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLDLGADTAKAMLQ
                     AADLGTNIQRLYQHELYFRTNGLQGERLNSVDLERLYQNMSVWLWNETKYRYEEGKED
                     ELGFKTFTEFLNCYTNNAYVGTQCSAELKKSLIDNKMIYGEESSKAGMMNPSYPLNYM
                     EKPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTG
                     LWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVK
                     FKVPYGGLIYIKGDSKDNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFV
                     YTAPKKNLNASNYTGGLKQFANDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHR
                     FANDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGA
                     TEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLK
                     EWAEKNFDIKKWYPEGSLPAFYSEREGMKGWNLFQLMHRKARGDEVGKTKFGERNYCA
                     ESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVGQSAYNTL
                     ASLKLPKPEQGPETINQVTEHKMSVE"
     gene            19813..>19994
                     /locus_tag="ACHKW5_003482"
     CDS             19813..>19994
                     /locus_tag="ACHKW5_003482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512817.1"
                     /translation="MSGWLFLRNFNKQKINNISQRNYINSLVVLTIKIAKATDRTVFA
                     HQANIINTDLFCVIKK"
BASE COUNT         4579 a         5598 c         4993 g         4824 t
ORIGIN      
        1 cattatgaaa attatcaccc gtggtgaagc catgcgtatt caccaacaac atccgacatc
       61 ccgtcttttt ccgttctgta ccggtaagta ccgctggcac ggcagcgctg aagcacatac
      121 ctgtcgtgaa gtgcaggata ttcccggcgt gctggctgtg tttgccgaac gccgtaagga
      181 cagttttggc ctgtatgtcc ggctgatgag cgtcaccctg aactgatgtt ccgtcacccg
      241 gaaaacgagt acgactggct gagctcagac gccgctgtgg atgaggtcct gctcatcaac
      301 gaatacacct ttaccgaagc cggtctgcgc gccggctgac ccgtcatcgt taaactatca
      361 ttcaccgatt ttctgtgagg attttatcgt gtcagacaca ctctccggga caacgcatcc
      421 cgatgacaat cacgaccatc cctggtgggg actaccctgc acagtgacgc cctgtttcgg
      481 cgtctgtctg gtgcaggagg gtaaccggtt gcattacctt gccgaccgcg ccggtatcag
      541 aggcctgttc agcgatgcag atgcgtacca cctggaccag gcctttccgc tgctgatgaa
      601 acaactggag ctcatgctca ccagtggtga actcagcccc cgctatcagc ataccgtcac
      661 gctgtatgcg aaaggactga cctgcgaagc cgataccctg ggctcctgtg gttacgttta
      721 tctggctgtt tatccgacgc ccgaaatgaa aaattaactc tccagaatag ccttctgcta
      781 cggcctggtg ttttcaccac gccactttcc catttctcat ctctgcatat caggaaaacc
      841 ttcagtatga aaacattacc cgatacacac gtacgggagg catcgcgctg cccgtctccc
      901 gtcatcatct ggcagacact gctcacccga ctgctggacc agcactacgg cctcacgctg
      961 aatgacacac cgttcgctga tgaacgtgtg attgagcagc atattgaggc aggcatttca
     1021 ctgtgtgacg cggtgaactt tctcgttgaa aaatacgcgc tggtacgtac cgaccagtcg
     1081 ggattcagcg cctgtaccca ctctcagtta ataaacagca tcgatatcct ccgggctcgc
     1141 cgggcaacag gcctgatgac tcgcgacaat taaagaacgg taaataacat taccctgggt
     1201 aaacatccag gggcgaaaca atgaaactgg ccctgacacc ggaagccgac agcgttaacg
     1261 tacaggcact gaacatgggg cgcattgtcg ttgacgtcga tggtattgag cttgctgaac
     1321 tgattaacgt ggtctgcgat aacggtcact cccttcgtgt tgttgatgaa tctgaccgag
     1381 cctcaacaga ctgcacgcca tcatttgctg ccctgactgg catacgctgc agtaccgcgc
     1441 atatcacggc aaaagacaac gcctggctgt actcgctgtc gcatcagacg aatgacaccg
     1501 gcgaatcaga atggattcat tttacaggca gcggatatct gttacgtacc gatgcgtggt
     1561 catacccggt tctgcggctt aagcgccagg gcctgtcaaa aacgttccgc cgtctggttg
     1621 tcacactcat ccgacgttat ggcgtcagtc tcattcatct ggatgccagc gctgaatgcc
     1681 tgccgggttt acccactttc gactggtaac caggaacaac atgaaatcat taaccacgga
     1741 aaccgcactg gatattctga ttgcgtggct gcaggacaat atcgactgcg aatcgggaat
     1801 tatctttgac aacgatgagg ataaaacaga ttcggcagca ctgctgccct gcattgaaca
     1861 ggcgagggag gatatccata ccctgcgcca actgcagctt ctgcaacaga accggtgagt
     1921 cttactcacc agacttcatt ccactgacgc cagcctgaat acggctggcg ttttcgttta
     1981 taaaccaaaa ggaaaaacga tgatgtcaga tatcacaatc tcccgcccgg aactggtcaa
     2041 cagacatacg gacgttatct gctcaaccag tatccgccac attttggctg tacgaaagag
     2101 tgcgctgctg caaatcgaca cacttatccg gcaactggct gaaatctcag caatgacaga
     2161 aagtattggc ggtaaaaccg ccccggcctg ggccatgaaa caggattttc gctgcggctg
     2221 ctggctgatg gagaaaccgg aaaccgcaat gaaagccatc acccggaatc ttgaccgcga
     2281 aatctggcgt gacctgatgc aacgttcagg gatgctttcc ttaatggatg cacaggccca
     2341 tgatacatgg taccagtcac tggagtacga taattttccg gaaatcagtg aagcgaacat
     2401 tctgagcaca tttgaacaac tgcaccagaa caaagatgaa gtgtttgagc gggggatgat
     2461 caacgtcttc aggggactga gctggaatta caaaaccaat tgcccctgca agtttggcag
     2521 taaaattatc gtcaacaacc tggtaaggtg ggacagatgg gggtttcatc ttatcaccgg
     2581 gcaacaggcc gatcgacttg ccgatctgga aagaatgctg cacctgttca gcggcaaacc
     2641 gatccccgac aaccgggaaa acatcaccat tcatctggat gaccacatcc agtctgttca
     2701 gtgtaaagag gactatgaag atgagatgtt cagcatcaga tactttaaga agggctccgc
     2761 acacatcacg ttcaggaaac cagaactggt tgacaggctt aatgatatta ttgccaggca
     2821 ctatcccgga gcgttagcga tgtaaaaagg aataaaactc aggaataaaa cccacaaaaa
     2881 tacacgtgtt ctgatgacaa aaatcttgat ctacttgttc tcataaaaac caccagaagg
     2941 gaccgagggt acaagagagg gtacattttt catcaaacca aataaacgag attaatatca
     3001 acatgataac aaccatattc gattccgagt ccggcaccac taattcttaa gaacccgccc
     3061 acaaggcggg tttttgcttt tggatctgac aataaccttc acgaaaaaaa ttagcttata
     3121 aagtctgggg gaattactct cgccacgtta acgagagtaa ttttattgat attaatctcc
     3181 tgatacttta cccccgtcca aactccagcc gctgcacatt caccatccca ggcttctcag
     3241 cagcactggc atcaatttgt gtcacccgca gcgcatactt ttcatccagc gcgtttaacc
     3301 atttcagcag gtcattaaac accaccggat ctatccagac ctgaatattc tccccacgat
     3361 cggctatccg cttgatgact accgagtgcg cggaagcact gtcactgatg acccgcgata
     3421 cctgcgcagg cgttgttgtg gcagattttc gcgctgcaat aatatccggc gcggcgttct
     3481 tcagtcgggt gttcatcgcc accagttgct gcaacattgt ctcctgttgc tcaatccgtt
     3541 cgctcaaagg ctgccagatg aggacgtaat atccgacgct aaacaggaac accaccgctg
     3601 ccagtaacat gcccttttca cgcggagaac gccccgccag gtgttgtgcc agccagtgtt
     3661 cgccacggct taactggcgt tcacgccatt gctgaaaata gtgaataaat ttatcgcgta
     3721 acatgttatt tcctccgcaa cgttacgccg ccggaaaccg catcaccctc tttctgtaac
     3781 gcgtcctgtt gcacaacata atcggttgcc agtgcactac gcagtttgtc gaaactggca
     3841 aagttcgcgg cccgtagctg gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca
     3901 cgcatttcga tgtcggaaag tgacgctgat ttcagggtgc tggctatcgc tgacaactcg
     3961 gcgagcagcc gggtatcgtc ggcctgtggg cgatattttt tcagcgccat cgtcacctga
     4021 gagcgtaaat tcacaatccg cttctgctcc gggaacaacg ttaagaactg ttcctccgcc
     4081 tgggtgcggc tttgcgccac ctgttcgctg acgctccata acgtcacgcc ccgctccact
     4141 gccagcgcaa ccagaatcag caatatctgc agaatcatca cccgccagcg cgcccactgt
     4201 tttcggtagc tgacgcgagg ctgccacggc cctgtcagca ggttcccttc cggtttgcca
     4261 taagtggtaa cggcgggcag agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat
     4321 gctgatagct tttccggtgc aatgccgact acggttagcg aaagcggtaa atcctgctca
     4381 ttgagctggg cgcggaacat gaccggagcc agcgccagcc cggcgctcca tccccggcat
     4441 tcatcgatgc ggcagataac ccgttgcgca tcgcaagcca taaacccaca aggaatggac
     4501 atccagtccg gcgcgacggt agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg
     4561 ttgcgcatat gctgttggtg aatcactgct acggttgcca gttgctggtc gatattcaac
     4621 ggggcgaaat gcagttcatc gatatcctgg ttcagctctt cttccagtaa ggcgggcaga
     4681 atggtcggta tctgcttacg ggcgacatca ggcagttcaa cctgccagac gctgatccat
     4741 tcgccgggaa tgtagagtcg aatcgcatcg gtttgcagcc attgctggag ctgttcgtta
     4801 gcaacgtcag accagatacc gtgctccaca tcgggtgtac ggcgctgcca atggatggga
     4861 tcagcggcgc aaagcgggaa aaaaatctca aggatggaac tcactcactt tctcctgtct
     4921 gatgccagag aaccgaaaag tgttgtgggc ccatgcggac aattaacgag ttcatcgtca
     4981 gttcagtctc attcacggtg atatctgaac gcagccagaa gtaattgctg tccacgctca
     5041 ggacggtttt tagctgtttt ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa
     5101 gaaactgatc gacatcttcc cagcccttcg ccggacgttg ttgtaataac gcccgcgcct
     5161 gaacggggct taaccacggg tcaaacagcg cctcaagaat cacactttgc gtgacgtcta
     5221 aagtattgat gttgatttgc tggcggatca tcggcagcgc acagaccagc ggtttcagtt
     5281 tttgataaag cccggcgtcc attccctgta ccacgcgcat ctcactgata tcagccagcg
     5341 gttgattcgc ggcgtagaaa ggcaccgaac gggcgagata ctcgctgtct tcacggccca
     5401 gacgtgtctg cacgctgcgg tcctcgtcaa taaactccca caggctttcg gctatcagtt
     5461 cggcccgata agcaggcaca tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa
     5521 gcggacgcga cgccgttgtc ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt
     5581 cacgcagggt gaccgcaatt tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc
     5641 acggctgtgc cagatgcacg cgcttttcat ttttcaggct cagactgagc gcgctcagcg
     5701 ccaggctttc cgcactggcg ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg
     5761 ttcgcccaag ttgttgctgc atccgcccgg aaagcgtgat ggttaccagc atcataaccg
     5821 ccagcaatac cagcaccacg accagtgcca ttccgcgttt tggtggtgag gtgatcatga
     5881 ttattgcggc ccgcgtaaca accagatgcg ttcaatctcg ccccattgcg gcgaatgcag
     5941 ggtcatgcgt accgccacag ggatcgcctg tactgacgac cagctctcct gccagcgcgt
     6001 gccgtcgtag aactgcaaac ggagcgaatc ggccggaatc aacttttgca ttgtcggctt
     6061 cacgctgcct gcggcatcgg tcagcggcca ggctaaccgt tcgagataac cgtcatgaat
     6121 gcggtaaccg acggtgagca gattactgcg cggcagacgc atcaacgggt taaccacacc
     6181 gccgcgcaca aaacgcattc cttcactctc agacgccagc acgccagcgc cagccagtaa
     6241 cgctggttca cgctggccct gatcgcctct taccggacgc ggcatcattt gggtcagatc
     6301 gtgggtcaga aaactcatcg tttgctgcat gaggtttagt ttttgatcgt gtccggcgac
     6361 ggcgctattc acgcgcgtga cgccgttagt cacctgctgc gccatcagtg ccagcgaggc
     6421 aaaaatggca attgccacca gcatttccag taacgtgaaa ccagcgcgag tccttctcac
     6481 tgttggcctc ccacggcgct aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct
     6541 tcgtgacggc tgacttcaat atccaccgcc tggagcagcg cgttgcctgt attcaatggg
     6601 attgttcgcc agtaccagcg gcttcctgcc atatcgctct caccctgatg cctgaccgcc
     6661 cgctgctcac gggtcatcag gtagagcagc gcagtctggt tttcagctac ccagcccgct
     6721 accgtttttt cctcaagaac gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc
     6781 gccgtggcag ccagcgcaaa aatcgccagc gcgagcatca cttccagcaa ggtaaatccg
     6841 cgcttcatgg catcctctca tcacattgat tgagggataa cgcgccatcg tgtgccagct
     6901 ttaccgccca acatgcgttt tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg
     6961 gttcaaaagg cgaaaaacgg atctgcggcg tcttcttttt cgcctctttt tgcagctcaa
     7021 gttcaatatc gtgcagcgtc agtcgacgac gttgcagctc cagcgcatat tccttctgcc
     7081 agatatcact tccgggttgt aacagcatct gcacctgttt cggcaccgta acctgtgccg
     7141 ataaccgggt cgaggaaacg ggcgaccact gcccctgacg acgctgcata aactggtagc
     7201 caggcgggtc gattagcaca ccgagtgttt tcccttcgat cactgccctg tccttaaact
     7261 gcgcaaagcg agtcagaaaa tcctgcgccg cttttttcgc gggcgattca gaatcggtcg
     7321 caaacgtctg tactacgccc gcactggcaa ggccgataag gaaaatcacc agcatgattt
     7381 ccagaagtgt gaatccgcgt tcaggcactg cttactgaaa ctcctgcaaa ttccagttac
     7441 cgatatctgc gccagcgcct tccccgtttt cctgaccatc cgcccccagg gtataaacat
     7501 caaacagccc tttttcaccc ggactgagat actgataatc attgccccac ggatcctttg
     7561 gcagtcgttt aatgtatcca ccagtacggt agttgcggga gtccgccata ttggctggct
     7621 gctggatcag cgcctcaagc ccctgttctg tggtcggata acgcccgtta tccagtcggt
     7681 acatatccag tgcgttttcc agcgccacga tatcgctgat ggctttttgc cgatcggctt
     7741 tctctttgtt gcccaacagg ttaggcacca ccagacttgc caggacgcca agaataacaa
     7801 tcaccaccat cacttccagc agggtaaaac ctgcccgtgg tttttgtgtg cgggataacg
     7861 aattcatgtg ataactccgt aaattacatt ccaaccatat tgttcagttg cagcatcggc
     7921 tcgaggatgg cgatgacaat aaacagcacc acgcccgcca tcatcaccac cagcgcaggc
     7981 tcaaacagcc ccaacgccag ccccacctgg gtatcaaatt cccgttcctg gttgactgcg
     8041 gcctgctcaa gcatggtttc cagttcgccg ctctgttcgc cggaggcgat catgtacagc
     8101 atcattggcg agaacagccg caactccgcc agcgcggcgc gcagactgct tccttcgcgg
     8161 acgcgatctg ccgccagcag tagttgttgt tcgacatagc gatttgccga cacggcagca
     8221 gcggtctgaa tgccttccag tagcggaaca ctgctggcgg tgaggatgct taaggtgcgg
     8281 gaaaaacgcg ccgtattcag cccgcgcgca acacgccccg tcacaggcag gcgcagcacg
     8341 gttttgtccc agcgcaggcg catagcagga tttttaagta gccgttgccc cagcaccaga
     8401 agcgccagca atcccgccag ccagtaaacg ccgctggcct gtaacgcatc actcatggca
     8461 ataagcgtgc gggtggagga gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt
     8521 ttcggcacca ctgccgtcag caaaatagtg actacgcccg ttgccaccac cagcagaacc
     8581 agcggataga gcatggcctg caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa
     8641 tctgccaggc gattgagcac cacgtcgaga tgcccggatt tttctccgac agcaaccatc
     8701 gaacaaaaca gagaatcaaa gacgcgggga tgttcgcgca ggctgtccga cagggtgtaa
     8761 ccttcctgaa tccggctgcg cagcgccatt ccgaggcttt ttacatgcag tttttcactt
     8821 tgctcactga ccgcctgtaa gcaggtttcc agcggcattg ctgcctgtac cagcgttgcc
     8881 agttggcgcg tgaacagcgc aagatctgcc gccgccacgc gacgatgtgc gtgccgccga
     8941 cgctgcaaca tcccccctga cgaagtattc atccgggctt caatatgcac ggggataagc
     9001 tctttaccgc gcaacaactg gcgggcatga cgcgcggaat ccgcctcaat catacctttg
     9061 gttttgcgac cattacgctc cagcgcctga tagtaaaaca gtgccattac gcctccatgg
     9121 ttacccgcag aacttcatcg agagaggttt ctccggcgag cactttctca atgccgttgc
     9181 tgcggatacc cgcagagtgt tgtcggacat aacgttccag ctccagctcc ccggcctgac
     9241 ggtggatcaa atcacgcaat gtggcatcca ccacgatcag ctcatggatg gcagtccgtc
     9301 cgcgaaaacc tttgtgatta caggcgggac agccctgtgg atggtacaga gtgacggtac
     9361 gggcgtcggt aattcccagc aggcgttttt cttcgtcagt ggctggtgcg ggctggcggc
     9421 agtcgggaca cagcgtgcga accagtcgct gcgccatcac gcccgtcaga ctggaagaga
     9481 gcaggaaagg ctccacgccc atatcctgca aacgtgtgat cgcccccacc gctgtgttgg
     9541 tatgcagcgt ggaaaggacc aggtgtccgg taagtgaagc ctggacggcg atttctgcgg
     9601 tttcggtatc gcggatttca ccgaccatca ccacatccgg gtcctgacgc aaaatcgcgc
     9661 gcagcccacg ggcaaaggtc atgccgacgc gggtgttaac ctgcgtctga ccgatccctt
     9721 caatcatgta ttcgatggga tcttcaaccg tgagaatgtt gcgcgaatgg ttgttcagct
     9781 cctgcaatcc ggcgtacagc gtggtgcttt tgccggaacc cgtcggcccc gtcaccagaa
     9841 agatgccgtg cggtttgtgt aacagctggc gcaactgcgc ggtcagttgc tggctaagcc
     9901 ccagacgctc cagcgtcagg cgggcctggt ttttgtccag cagtcgcagc accacgcgct
     9961 cgccccaggc agaaggcatg gtggagacac gcacgtcaat cgcccggccg cccagcaaca
    10021 gcgcaatacg tccatcctgc ggcacgcgct tttcggcaat gtccagccgc gccatcacct
    10081 tgatacgcga caccagcagc gaggccagtt tgcgccccgg acgcagcatt tcatgtaatg
    10141 tgccgtcaac acgaaaacgg atcaccagac tcttttcaaa cgtctcaatg tggatatccg
    10201 aagcgccttc tttgattgcc tctgccagca tggcgttgat cagtttgatg atcggcgcat
    10261 cgtcgtcgct ttccagcaga tcttccgttt ccggcagttc ttccgcgagg gtaaaaaagt
    10321 cctcggcaga accgagatct tccatcagct ggcgagcctc ggaagagtcc cgctgccaga
    10381 ccgcattcag ccgctgttca aattcggcct cgtcgatttg ccgcagcgta aagggcgcgt
    10441 tcagcccccg ctgcagctcc tgcaaaacag agagcgatag cggatgaacg tggaggatct
    10501 ccagcgacgc ttcgcaccat gccaccaggc taaaccgacg gctgaaactg tagggcagac
    10561 gcacggtgtt ggcggtggtt tcctgtgcta caggcaccat taacgcgttc tcccggcatt
    10621 gaggaacgcg cgaacttccg gcggtaaggc ctggttttgc gctggcagta ccggctgtgc
    10681 ggtgtgcggc atcaggctta aaccttgctc atcacgatag atctgttcgg cgcgcatata
    10741 gttatatttg cgctgcgaca cgccgtctgc cgccataccg tcacgcagaa tggtcggacg
    10801 gataaacacc atcaggttac gtttttcttt tttatccgcc gtcgatttaa acaggttacc
    10861 aatcaatggg atatcgccca gcagcggcac tttcgccacg ctttctcccg cctgatcgtc
    10921 catcagacca ccaagcacaa tcagctcacc atcgttagcc agcacggtgg ttttcagttt
    10981 gcgctcacca aacaccacgt cgaggctggt ctgtccttcc accttcgaga cttcctgctc
    11041 aatcaccatc tgtaccgcgt taccttcgtt aatctgcggc gtgactttca gcatgatgcc
    11101 gacttttttc ctttctactg tattgaaagg attgctgtta ttggagccaa cggtagagcc
    11161 ggttaatacc ggaacgtcct ggcccaccat gaagaaggct tcctggttgt ccagcgtggt
    11221 gatgctcggc gtggagagca cgttcgagct ggagtcgttt ttaaccgcct gcaccagcgc
    11281 catccagtcg cctttcacca caccaaccgc cgtaccgcta aagccggaaa gaagctgggc
    11341 gagtgtggag agatcgccgt tggtatccgg attaatggtg gtagcgccgt tttcactgat
    11401 caccgtcgag cctttctgcg gtttcgcctg agaaatggct gcccccagcg taccgatagg
    11461 gatctgcgta ccattagcaa actgcattaa tccggcatct ttcgacgccc actgcacgcc
    11521 gaagttgata ttgctacctt cggcaacttc cacgatcaac gcctcgacgt gcacctgagc
    11581 acggcgaata tccagttgtt caattacgct ttgcagcgac tgcatgatgt cctgcggcgc
    11641 ggttacaatc agggcattac tgtgtttgct ggcggcgatg gatacaacct cacgcccgct
    11701 accgaccgtg ccttccgcct cttctttagc cgccgtgagc gtgccgctga cctgtttcag
    11761 cacatcgacc agatcttcgg ctttgctgta tttgagatag aaaacctggc tgttgccgct
    11821 gcgttccatt tctgagtcca gccgacggat caggcggcgc attttgtccc gcgtggccgg
    11881 gtcaccactg acaatcacac tgttggtgcg ttcgtcggcg acaatttgag atttcagcgt
    11941 cgcaggctgg ttctcgccgc tgtttttcgt caggctttcc agcacgcggg cgatttccga
    12001 agccgaggcg ttatccagcg ggatcaccta ttcggtgcga ttacccgcgt gatccacgcg
    12061 ctggatcact tccgtcaggc gctccacaac ggaggcgcgt ccggtgagca taatcacgtt
    12121 ggagggatcg taattaacaa cgttgcctga gcctgcgctg tcgatcatct ggcgcagaat
    12181 cggtgccagc tcgcgtaccg acacattacg taccggcact actttggtga ccatttcatc
    12241 gcccgcgtag ttgtcgctgc cttcaccgac cagcggcagc ggctcgactt tcgcagcgct
    12301 ggatttcacc accttcagca cgtcgttttc catcggtacg acggcatacc cctgtgcttc
    12361 cagcaggtta aggaatagct ggtaatactg gcgttcattg agcggggtca tggtgcgaat
    12421 actcactttc ccctgtacgc ccggccccat aatgatggtt ttattaaggt tagcgccgac
    12481 ggtttcgatg aacgatttca ggtcggtatc tttaaaatta gcggtgaaag tggcttcttc
    12541 cgcccagacc ggtgaactgc ataatgccgc tgccagcacc agcggcagta aacgcttttt
    12601 tgttttgagg ccagttgtct tcttacgcca gaccgacaac gtaatatcac gccaaaacac
    12661 gatgaatgat tctcctggat taaatgcggt tagcgcagcg cgatggaaat gtcgtggcgc
    12721 gcacccttgc gtaaaaccgt aagttgaatg gaatccattg aaggtaactg ccgcatcaga
    12781 gcaatcattg ctcgtggatc agtgaaatcc tgctgattta gcgcaatggc gatatcgcct
    12841 tccttgaaac cgctggcatc gaacagagaa cgatctgccc ccggcttcac tgcataaccg
    12901 acaattccct ccttacgcac aggcgtaagc tggatatagt taaaaatttt ctgcggatct
    12961 ttcgccagtg cctgacgcac ggcagtcggg atctccactg gcgcactgac agcaggttca
    13021 gcaacagctt gctttgcttc gtcactgacg gcttttttgt tggtcacggc tacggtggaa
    13081 tgcccctctt ctgccaggct caggcgctct atttttccct gatagcgcag catcacatgg
    13141 tcgcgattga tttcctcaat caccgcattg tgcgagccaa gcgtttcacc ctgcaaatag
    13201 acctgctgtt taccgccttc ttcaataacc gcgccgggtc tggcaccaaa ggcaatcccg
    13261 cgcagcacca cattaagacg cgtttctgcc acaggcacag gttcgggttg ttttaccggc
    13321 gtggcgacgg gctgatattt gccaaaccag ttttgctggc tgattaattg cacgtcattt
    13381 ttatcgaacg tttttgcatc cgcacggggc ggtttattcg ccgatgggga aacagccgta
    13441 tattccgcag aaaaggagaa atagcgccag agtgaatgcg ccacttttgc agaaataata
    13501 agcagcatca gccagaacat cccgcgtaca atttttcgca gatgctcttt gtctgtatta
    13561 aaagattgtc tctggttaag agtgtgacga atttttgtca gccattgaat gagataaatt
    13621 cgtgcgtcac gaaaaacaac ccgcgccaat ttatatctac ccgacgttat gctttgacta
    13681 ttccacaggt ggtacgatcc agtttccgct gatactggtt acctgtacgt gtatcattaa
    13741 tcgttatgct gtaattaatt ccctcggtaa tcattaattt caccgttggg tcagcgcaca
    13801 tttgtcgttg atagctggtt aaaaaggcgt caggcgtctg cgtggtctga gtccccgctt
    13861 cgctgataat ggtcattttt accgtcgtgc cgctactttg tgccagcact aaggtatatc
    13921 ccgcagattt aatcggcagg ttttggctga tattttgcgc ctgttttttc gccagtaaac
    13981 tggcattttc attatggctg gcacagccac ttaataatcc tgtaacgctc aacaatagcg
    14041 atattaatac cctccctggc atttgtttta tcgacatagt aaataatctc taaggttatt
    14101 aataagagtt aaaatgtcac tttgataatg acgtggttat cattaaaaca atgcctgtag
    14161 ataaagtgtt gctataccgc ccagacttaa acacggtcca aaaggcagtg tggttgatcc
    14221 tctttttgta ataatggcat atatcaggcc gcagcatgat gcgattaaag caacattggg
    14281 cagtgataac ggccccaccc agccacctaa cgcagcgaac aataatacat cgcccatgcc
    14341 taatgcttct ttacgcagaa ctattccggc tatccagcgc agggagtaaa aagtgataaa
    14401 ccccaccagg acgccggtaa ctgcgtcttg tagcgttaac ggactctgtt gcgcccatgc
    14461 cgcaatcagt cctgtccaca atacgccctg agtaaaaaca tcgggcagcc attggtgatc
    14521 gaggtcgatg atgctcgcgg caatcagcca ggcggataat atcatcaccg ccagcgccca
    14581 tccactttcc ggccagacca gactcgccag caaaaaagcg agtgccgtca ataactccac
    14641 cagcggataa cgcttgctga ttttcgcctg acagtcgcgg cagcgccctt tgagcatcaa
    14701 ccaggagagc agcggaatat tgtcacgaac gcggatggtc tgctgacaat gtgggcagtg
    14761 cgaacgcggt agcgcaaggc ttatttttga ctgcgcactc gacatttcac cgtgaaactc
    14821 cgccatttgt tggcgcagca tgatggggta acgccaaatc accacattca aaaaactgcc
    14881 gatgatcaat cctccgacgg ttgccagtat gggcatcgcc gcggggtatt gctgaaaaac
    14941 atcaaaaagc atggttaaag gttgtttgtt gtaacttgct ggatgcgggc ctgcaagtgt
    15001 atgccatacg gctttcctcc aggcccgata agctggcgca acccggtacg ccttatttca
    15061 tgccggatgc ggcgtgaacg ccttatccgg catacaggat tactcgacag acatcttatg
    15121 ctcggtaacc tgattaatgg tttccggtcc ctgttccggt ttcggcagct tgagcgacgc
    15181 cagcgtgttg tatgccgact ggcccacacc gccctcgaag ctcatctcgc tcgcccccgg
    15241 cagctggtaa gcattcgcac ccggattcca tttcttaaag aactccgaaa gatccgtctg
    15301 ggcgacccag gaggcacaca gcatcagcgt gtccgcagcg ttaccgtttg actcagcaca
    15361 gtaatttctt tcgccaaact tggttttacc aacctcatcg ccgcgtgctt tacgatgcat
    15421 caactggaac agattccagc ctttcatccc ttcacgctcg ctgtagaacg ctggcagaga
    15481 gccttctgga taccatttct tgatatcaaa gtttttctct gcccattcct tcagttgtgc
    15541 gtacatcagc agacggtcac ccgcaccgcc gcgcgcccat gcctgaccgt tgctctcctc
    15601 cagatattcc ggtgcgacgg taatatcgtc agcgacacgg ttcatcttgc cgagataacg
    15661 atcctgcatg tacagcgcca gcacgttgtt cgccacttcg gtcgcgcctg gaacagtcag
    15721 cggcgtttct gcggcgttgt gccctacttc gtgccagatc agccagtcgt tcagcggcgt
    15781 cgtcggcagc gtggtgctgt tcgtcgagaa gctgctgttc attaccggat aacccgaatg
    15841 cgcatcaccg atggagatct gcacatcgtt ggcgaaacga tgcttgtggc ccgtcaagtt
    15901 tttataggta aacatccggt gcttaccgtc ttcatcatta cgaccgtaga aatcattcat
    15961 cgagctggca aaggtatcca gatcgttagc gaattgcttc agtccgccag tgtaattgct
    16021 ggcattcagg ttcttcttcg gtgcggtata aacgaaagcg tctgattcca gctcacccag
    16081 cggagccggt gagttcagat cgtttttcca tgcgccgtct ttatagaacg gtgcttttac
    16141 cacaccagta aaggtgaagc tggcagattc attgtctttg ctgtcgcctt tgatataaat
    16201 cagaccaccg taaggcacct tgaatttcac cgtaccacta gcgtccagag agtacgtttt
    16261 ggttactctt ggcggacggt tcagcgcgac ttcatgcttc tctcttccgg tcaggtcgtc
    16321 agccagcgcc acggtgacgg tcacaggaac gttcgcattg gacttaatgg tgacctcttt
    16381 ctgagccggt gcccacaggc cagttgactg catgttacct gcaaaccatt tggtcggatt
    16441 cgagtacagg ctgatggttt cagtaacgtt ctgtccctct tccgatactg ctcccggata
    16501 cttctcgaca tcaactttga tgttcagatc ccaccaggaa cggcccagca tcaggcgtgt
    16561 cagcggtttt tccatatagt tgagcgggta gctcgggttc atcatgcccg ctttgctgct
    16621 ttcttcaccg taaatcatct tgttatcgat cagcgatttt ttcagctcag cggaacactg
    16681 cgtgccaacg tatgcattgt tggtgtagca gttcagaaac tcagtgaacg ttttaaagcc
    16741 cagctcgtct tctttaccct cttcataacg atatttcgtc tcgttccaca gccagacgga
    16801 catgttttgg tataaacgtt ccagatcgac gctgttgaga cgctcaccct ggaggccatt
    16861 ggtacggaaa taaagctcat gctgatacag gcgctgaata ttggtgccta aatccgcagc
    16921 ctgcagcatc gctttcgcag tgtcagctcc aagatccagc tgcgtatact gcggaacata
    16981 catgccaccg gttaccggca cacccgtgcc tgggcggtat tccagacagt tgacctcata
    17041 gtgatagtca gaatccttac acacttccag ccctgggaac gcgtccagaa ttctctgttt
    17101 tgccgcagcc agtgactcag gcgttttgtg gtcggcttca tcaataaagg cgaattgggt
    17161 tacctgttta ccctcaactt cttcctgcca gctggcaact tccagcttcg gtttgtcatc
    17221 aggtttgttt tcttgctgat atttccagat aacttctttc gtggtgtcat caatggtata
    17281 cggtggttta ccatcgacag ccggataacg ctcatagacc caaattggcg ttgaacgtcg
    17341 ttgacgaaca cggtcagggt agccttgcgg atcgttattt actaccgact tgttaagcgc
    17401 catcgacaaa cctgcggcat caagcagacg tacaaagcca gatgcgctct cttccttaag
    17461 attgctcatc acgttttcca tgatcagcac cgatccacct ttgttcatat aggcgatcaa
    17521 atcggtcaca tcctgctggg tcagcttcgg tttgctggta tctgcgcgca gaggaatgga
    17581 gtaaggatcg ctaccccatt gtgtgacgta ttcaaagcca ttgaggatca acagaggaac
    17641 ttcatctgga ttcagattgc catagctggt cattggtttg accgtgatgc cagtgaaatc
    17701 cttgtggaac gcaaacggtg cgctatttcc cagcacctga ccatgttttt tgaaatatac
    17761 cgtgtccagg ttagtaccca cggtcatact ggattttgca tccggcaacc agcgatcatt
    17821 tgacagatag cgcagcacgt tctccatgaa gttcttcatg tcatccgggt cgctgttgag
    17881 cgtacactgt ccgtctttat taacgccccc gttccagctg taaccgttcg ggcaacgcag
    17941 gatgctgttg tagtgtgggt taccgataac catcagcttg ccatcgccca cttgccccag
    18001 cgagataaac ggcaggttga aggtggcggt ttcgcgtgtc acgttctctg gtcgcacaat
    18061 ggaaggcgct tcagtaatgt acgccagctc atttttatcc caggcgcgtt tttcgccaaa
    18121 cgccagccag tagtttttat cattacgcgc catcagaatc gggaaggccg cgttggagat
    18181 attcaccacc gcctgaccgc gcgcattacc cgtgctgcca tagaagttag tagagtcatg
    18241 gaatacatgg aacttgctga cagatttgta gttcgtatcc acgccccaca gcttgttgat
    18301 aacgccctga atctggccgt cattaacatt gcgcgtcgtc agcgagaacc agcgagcctc
    18361 gttacaaccg tcggttttcg cacaaatcgc ggtatcgatc tctttggcct gacccgtctt
    18421 aaactgctcg ataaattcgt ttggcagatt aacaacttgc tcaccttcat ccagcgtcgc
    18481 accgttggat aacgagagat tgataatctc gttgatcacg ttgggatatt cggcaaagac
    18541 cttgcgtaca tcatccggaa caacacgggt attattttgc ccggtcgtcg aatagcgatg
    18601 aataagctga tcgatattcg ccccgcgaac ttcatcaccc agttcagtca gcgcaatggt
    18661 cgacttattg ccgcgcactg aacccagttc aaaggtatcg ataccaaagg agatggtttc
    18721 gccccagcta aaagaaaatt caccattttc ccctgtcacg ccacggcctg aattggtgta
    18781 gtagttgacg ccagcaacac catatccctg gctatccacc aggcgacctt ctgagagaat
    18841 gatttccgaa ggttgatact gataaaactg ttccgcgtta gccgacacga aggaggcgtt
    18901 cagatccggt tttgttcccg gagtcgtgac ggggactacc ggtgaagtat gagtggatgg
    18961 cgctttatca gtcgcggcat tgttttccac ctcttcattg accagctttt tgaactcttc
    19021 cggtgccaga tcgatttgct tatacagcga gtcgaaacgt ttactctcga tcaccgagga
    19081 gaactccagg cacacctgtt ctgtattcgc cgggcaactg ttcatggagg tgaccagcga
    19141 gagcgcattg cttttcttgt tgtcggaacc cgccagttct tgcgcgtcct caagactaaa
    19201 cgacactttt tcaaccgcac gcaggctacg cgcagcttct gactgggtgt cgaaggtagc
    19261 aattgtcgtg ttgttccctg ccacacaggt gactttgtcg cctggtgtaa aggtaaagcc
    19321 atcgctggat tcatcattac aggtgatatc accagttacc cgcaggcttc cgcccagggt
    19381 cagataaccc gttttcgtag gaacaggttc tggctccggt tctggtgttg gctcaggatc
    19441 aggtgtcggt tccggcgtag gttctgggtc cggcgttggc tcaggcgtcg gctccgggtt
    19501 tggtgttgga tcaggtttca cttccggcaa agaccctgtt ccagaatcta caggcggcgt
    19561 atcggaggaa gatccggagc caccgccatc acaaccggct aacagggttg cactcaaaat
    19621 agccgctaaa agcgatttct tatatttaaa tttcttattc attaataacg caagtgacaa
    19681 aacaatgtta aaaaattcgt aactgggaga aatagtttta tgctttatct tctttaataa
    19741 tttcctccat tcttaagaaa acgacatcat tgatagaaaa caggtgaaat ttataagaat
    19801 aacccctatt tgatgtctgg ctggctattt ttacgaaatt tcaataaaca aaaaattaac
    19861 aacatttcac aacgcaacta tataaattcg ttggtagttt taacgattaa gattgcaaaa
    19921 gcaacagata gaacggtgtt tgctcatcaa gcgaacataa ttaatacaga cttgttttgc
    19981 gtgataaaaa aata
//
LOCUS       DAZFYC010000087        19949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFYC010000087 DAZFYC010000000
VERSION     DAZFYC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.087"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            249..749
                     /gene="tssB"
                     /locus_tag="ACHKW5_003483"
     CDS             249..749
                     /gene="tssB"
                     /locus_tag="ACHKW5_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7512818.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            784..1008
                     /locus_tag="ACHKW5_003484"
     CDS             784..1008
                     /locus_tag="ACHKW5_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512819.1"
                     /translation="MTAHKNISETYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1059..2534
                     /gene="tssC"
                     /locus_tag="ACHKW5_003485"
     CDS             1059..2534
                     /gene="tssC"
                     /locus_tag="ACHKW5_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7512820.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPWSPADNPVKSFNYHEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2541..2954
                     /gene="tssE"
                     /locus_tag="ACHKW5_003486"
     CDS             2541..2954
                     /gene="tssE"
                     /locus_tag="ACHKW5_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7512821.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2958..4808
                     /gene="tssF"
                     /locus_tag="ACHKW5_003487"
     CDS             2958..4808
                     /gene="tssF"
                     /locus_tag="ACHKW5_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7512822.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4772..5854
                     /gene="tssG"
                     /locus_tag="ACHKW5_003488"
     CDS             4772..5854
                     /gene="tssG"
                     /locus_tag="ACHKW5_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7512823.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5879..7159
                     /gene="tagH"
                     /locus_tag="ACHKW5_003489"
     CDS             5879..7159
                     /gene="tagH"
                     /locus_tag="ACHKW5_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO7512824.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7156..7680
                     /gene="tssJ"
                     /locus_tag="ACHKW5_003490"
     CDS             7156..7680
                     /gene="tssJ"
                     /locus_tag="ACHKW5_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7512825.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7683..9014
                     /gene="tssK"
                     /locus_tag="ACHKW5_003491"
     CDS             7683..9014
                     /gene="tssK"
                     /locus_tag="ACHKW5_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7512826.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9019..9780
                     /gene="icmH"
                     /locus_tag="ACHKW5_003492"
     CDS             9019..9780
                     /gene="icmH"
                     /locus_tag="ACHKW5_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO7512827.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYIPCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9789..12578
                     /gene="tssH"
                     /locus_tag="ACHKW5_003493"
                     /gene_synonym="clpV"
     CDS             9789..12578
                     /gene="tssH"
                     /locus_tag="ACHKW5_003493"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO7512828.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLTMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRL
                     AHLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGD
                     TIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGR
                     QYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHP
                     DVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPV
                     LADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDE
                     IMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVE
                     DAQNDESVTKDETVL"
     gene            12575..13318
                     /gene="vasI"
                     /locus_tag="ACHKW5_003494"
     CDS             12575..13318
                     /gene="vasI"
                     /locus_tag="ACHKW5_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO7512829.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13323..14735
                     /gene="tssA"
                     /locus_tag="ACHKW5_003495"
     CDS             13323..14735
                     /gene="tssA"
                     /locus_tag="ACHKW5_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7512830.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14844..18278
                     /gene="tssM"
                     /locus_tag="ACHKW5_003496"
     CDS             14844..18278
                     /gene="tssM"
                     /locus_tag="ACHKW5_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO7512831.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLASRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGSRGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHTLGWLKEKRARQPLNGIILTLDLPDLLTADKRRREYLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRDWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGKDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWMPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMNSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDAGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18289..19738
                     /locus_tag="ACHKW5_003497"
                     /pseudo
     CDS             18289..19738
                     /locus_tag="ACHKW5_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19665..>19949
                     /gene="tssD"
                     /locus_tag="ACHKW5_003498"
     CDS             19665..>19949
                     /gene="tssD"
                     /locus_tag="ACHKW5_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO7512832.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKA"
BASE COUNT         4682 a         5487 c         5543 g         4237 t
ORIGIN      
        1 aattattgcc acaatacacc tgatgcgaat tgcacaggtt cacacaatct atttaacgct
       61 tgcagaatac ccctctttac gatcgataaa aagtcctttt cttatttatt ttgaagcgta
      121 aaattcgggg tgatgaagga gtggtgattt tattttcacc gccattaggg cggcattgcc
      181 gatatttttt tgcggtacaa atatgccgtg ttcatggcct gtacgagata acatgacgga
      241 tcacttttat gagcaaaaaa tttgaaggca gcgtcgcgcc tcgcgagcgt attaatatat
      301 cgtatgtccc aaaaacagat ggccagacgg ctgaagtgga acttccgctt aacatgctcg
      361 ttgtcggcga taccggaaat acgcaggaaa catccccact ggatgaacgt caggcagttt
      421 ccgttaacaa gcacaatttc ggtgcagtga tggctgaagc agccattggc ctcaatttta
      481 ctgtccccgc cacgctgaag ggaagcacaa cagacgatga actgaatgtg gcgctgaata
      541 tcaaatcact ggatgatttt tcaccagata gcgtggcccg gcaggttccg gaagtgaata
      601 aattgctgga actgcgtgaa gcactgactg cactgaaggg gccgatgggt aaccttccgg
      661 cattccgtac ccagctccag gcattgctgg aaaatgaaga gtcccgcgag cagttgctca
      721 aagaaatcgg tctggtgagc aataaataac caccactatt tcattaaaca gggttatcca
      781 ggcatgacgg cacacaaaaa tatctccgag acataccacc tctctgttgc agacattctg
      841 caggtggttt accaggtatg tttcagcccg tccgttgaaa tcaaccagga tggtgtggct
      901 gcgctgatta caacactgga tcgccgtatc agtgatctat tggatgagat tatccatttt
      961 tgtgagtttc agcagagcgc gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc
     1021 ggtttcagaa acacagctaa aacagaagga ttttcagtat gtctttacag gaagaggaac
     1081 tcgtttccag tcatgccggg cagcctgagc aggcatcgtc gctgcttgat caaatcatgg
     1141 cacagacccg tattcagccc ggttcggaag gttatgacgt tgcccgccag ggtgtgacag
     1201 cctttattgc cagcatcctg caatccactg catctgccga accggtcaat aaactggccg
     1261 tggacagcat gattgcagat attgatgaac gcatcagccg ccagatggat gtcatcatcc
     1321 atgcgcctgc ctttcagcag gtcgaatcgt tctggcgctc actgaaaacc atggtggatc
     1381 gcgttgattt ccgtgaaaac atcaaggtca acgtgcttca tgtcaccaag caggaacttc
     1441 tggaagactt tgagtttgcg ccagaaatta ttcagtccgg attttataag cacgtttatt
     1501 cgtccggatt cggacagttt ggtggtgaac ccatcgctgc cgtgctgggc gcttatgagt
     1561 tcaaaaatac cgcacctgac atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg
     1621 cgcacgcacc gttcctgtcc tccgtttccc cggagtttat ggggctgaac tcgtggaccg
     1681 aactgcctaa tatcaaagat ctttatgcca tctttgaagg tccggcctac accaaatggc
     1741 gcgccctgcg tgactcggaa gattcgcgct atttagggct tactgcaccc cgcttcctgc
     1801 ttcgccagcc ctggtcacca gcagataacc ctgttaaaag ctttaactat cacgaagatg
     1861 tcagccagaa ccacgaagat tatctgtggg gtaatacggc ctggatgctg gcatgcaata
     1921 tcgcagacag ttttgccaaa taccgctggt gtccaaacat tatcggcccg caaagcggcg
     1981 gcgcggtgaa agatctgccg gtgcatctgt tcgaaacgat ggggcagatt caggccaaaa
     2041 tcccaaccga ggtgctggtc accgatcgcc gcgaatttga actggctgaa gagggtttta
     2101 tcaccctgac catgcgtaaa gactctgata atgcagcctt tttctctgca aactcggtac
     2161 aaaaaccgaa gcactttccg ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc
     2221 ttccttatct cttcatcatc aaccggttag cgcactacat caaggtgttg cagcgtgaac
     2281 aactggggtc atggaaagaa aggagcgact tagagaggga gcttaatacc tggatccgtc
     2341 agtatgttgc cgaccaggaa aacccgcctg cggacgtgcg cagccgtaaa cctctgcgcg
     2401 cagccagagt tgaggttatg gatgtggaag gcgaacccgg ctggtaccag gttgcgctaa
     2461 gcgtgaggcc tcattttaaa tttatggggg caaattttga gctttccctg gttggccggt
     2521 tagacaggga gtaacacacc atgataccgg atcgggaaag aaccacaggt tctctttttg
     2581 agcgtatgga agcctcttcg gccagaaacc ggcagggagg cagtattcat tcgctcaggc
     2641 aatccattcg ccagaaccta cgaaacattc ttaatacccg gtccggtagt tgccggggtg
     2701 cgcctgagct gggcattgat gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc
     2761 gggccatcga gcaatgtata gaacgctatg aaccccggat ctcacacgct gaagtgcagg
     2821 cagtggtttc atccgcctcc agcccgctgg atatgacgtt tcatattaca gcctgggtga
     2881 catttaacga aacgcatgag gtgcttgaat ttgatatggc accgaatggc agccagcatt
     2941 accgggtgga ctgacaaatg gaatttgaag aacgctattt ccgggaagaa ctcgattacc
     3001 tgcgccagct tagcaagctg ctggcaacgg aaaaacccca tctggcccgc ttcctggccg
     3061 aaaaagatgc ggatccggat attgaacgcc tgctggaagg ggtggctttt cttaccggca
     3121 atctccgcca gaaaattgag gatgaattcc cagaactgac gcacgggctt attaagatgc
     3181 tatggcctaa ttacctgcgt ccggttccgg caatgaccct tattgaatat acgccggata
     3241 tggataagtc ttctgtaccg gtgttaatcc cccgtaatga gcagtttaca accaacgccg
     3301 gggaaatcag agttgatgaa gtgctgccct ctgatgctaa aaaggaggag ccgcctccct
     3361 gtaccttcac tctctgccgg gatatctggc tgctgcccgt tcgcctggag cagattgaaa
     3421 accgcagtac gacccgtaat ggtgttatca acatcacctt ttcggtcgca ccgggaacgg
     3481 acttccgcac gctggatctg aacaaacttc gcttctggct cggcaatgac gacaactata
     3541 cccgtgacca gctttattta tggttctgcg aatacttgca gggtgccgac ctgactgtgg
     3601 gtgaacagca tattcgcctg cctgagttta tgctaaaagc tgtcggtttt gagccgcagg
     3661 atgccatgct gccctggccg aaaaacgtcc acagcggcta ccggatcctt caggagtatt
     3721 tctgttaccc cgatgcgttt ctcttttttg atctttgtgg ttgtccggct ttgcctgacg
     3781 gattgcaggc ggaattcttt accctgcaac tgcgtttttc gcgccctttg cccgtggaca
     3841 tccggctgcg ccgcgattcc ctgcgcctgt attgcgcacc tgccattaat ttatttatcc
     3901 accatgcaga agccatcacg ctggacaacc ggcgggcaga ctatccgctg gttcccagcc
     3961 gccattaccc acaacattac gatgtatttt ccgttaacag tgtggtgagc caggtccagg
     4021 atatgttcag gaaaaaagat ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc
     4081 cagcctttga aagtttcagc catcagatgg aatacagccg gaagcgggaa gtggtgtact
     4141 ggcatcaccg gaccaaaaca tccctgttcc atcgcggctt tgatcatacc cttgccttta
     4201 tacatgctga tggcagttat ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg
     4261 tatcgctgac ctgtaccaac cgtgagcttc cgtcacaaat acgttccggc gatatcaccg
     4321 gcacaaccgg taaaaatgcg gctgttgctt catttcgcaa cattacccgc ccgacgcaac
     4381 cactctggcc ggtcattgat ggcagcctgc actggtccct actctccgcc atgaacctga
     4441 attatctgtc attactggat acggacgcgc tgaagcaggt catcgccaac tttgatcgcc
     4501 acgcaatcca tcatccgcag acggcacggc tgtcacaaca aaagctggat gccattgagc
     4561 gtctggagac ccgccccgtt gatcgcctgt ttacgggtat tcccgtccgg ggactggcct
     4621 ccacgctgta tctgcacccg gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta
     4681 cggtgctttc gcattttctg tcgctgtacg ccagcgttaa ctcattccac atgctgaccg
     4741 ttgtgaacac agaaagccag gagacatgga aatggacgga aagaatcggg cagcatcctc
     4801 ttatctgagt ccgggaaatc ccccggcaga taaagagcag aatgatccgc tggcacaggt
     4861 tttccataat gcctgctctt acaatttttt tgcgatggcg gagctgttgc accgcctggc
     4921 aaagggtgaa aaggggacgc cagaattatc cctgcgtgac gatccggcac aggaaaccct
     4981 gcgtttcagc gccgatgcca gccttgcctt cccgtgcagt gatatcagtg cactgaaacg
     5041 ggatacgtca ggggcattca ggatgaccac cacctttatg gggttacagg ggagccagtc
     5101 gcctctcccc ggttattacc tcgatcacct ggcctggaaa gcggtacatg aacaaagtcc
     5161 ggtaggtgat tttctggata tgttcagcca ccgcctgacc cagtttgtct ggcatatctg
     5221 gcgtaagtac cgctatcaca tcagttttcg taacggcggt gtggatgcgt tctcgcagcg
     5281 catgtactcc cttgtcgggc tgggccaccg ccagcttcgc gataagctgg cgattaatca
     5341 cagcaaaatg ctggcgtatt ccgggatcct ggcaaatccg ggacgatccc cggaaataat
     5401 ctgtggcctg gtgtcccact gctttgattt gtcagaagtg acgctgcaaa actggcaacg
     5461 tcgcaaggtg gatattgaac cggatcagca aaatagtctg gggagctatt ctcttaaaaa
     5521 tggcgaaaaa ctggcgggaa gatcggtact gggcaacttt gttctgggta cacgagtgcc
     5581 cgatctcagc ggaaaattcc agctcagtat taccagcctg acccgaaaac agttcctctc
     5641 ttttctgccg tccggcgaaa acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg
     5701 cgatcagctt gcctgggact tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg
     5761 cctgggcgat aacaaaagtg cgctgctggg ctggaccagc ttcctcggca caccggaaga
     5821 acgaccctca gtcaccatca gggtacggtc gtaaattatg ataatcacgg agcagaatat
     5881 gccggaagag aaactacaga cgctttcatt gcaggtcatt aacggcagtg agctggaaag
     5941 cggacgggcg gcgcgctgtc tgttcacaca gcagggaaat gtcggccatg gccccgaatg
     6001 ccactggtcg gtacaggatc gtcagcagag cattccggcc caggctttta ccgttatcct
     6061 gcacgatggc acattttgtc tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa
     6121 agtcacagca acatcagacc taatacagtt gcgccagggc gatgagatcc agatcggacg
     6181 gctgatggtg agggttcatc tgaaccgggg agatattccc cattacgatg aggaaatggc
     6241 cactcccgaa accatcgtta ccaatcgcga tatgctcacg gataccctgc tatcaacgga
     6301 gggtacgcca cactatccgg gaatgactca ccggcaccaa cttgcagaca ccgtggtaaa
     6361 cggtttttct gccgatccac tccaggcact tcagtccgaa agcctgatta ccacgggcga
     6421 tccgctttca ggcattgcgg ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa
     6481 tggggggatc aatactccgt ttatggatct gccgcccatt tatgccagcc ccggcgatcg
     6541 taatgacgac gtctctgcgg cagaaatggc gcaacgccac cttgcggtca cccccttact
     6601 gcgcggtctt ggcggctcgc ttaccatgag caattccgac gatgcggatg attttctgga
     6661 ggaggccgga cgaacgttac aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg
     6721 taacagcctc tcagacaaac atttgcgccc gctggaagat aacccgctgc gcctgaacat
     6781 ggattacgcc accgcgctcg acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc
     6841 ggctcccgcc gccgtcagtg aaagcctgcg caatgtccgc caccacgaag aagctaaccg
     6901 ggcagcgatt gtggagtcgc ttcgtgtcct gctggatgct ttctcaccac aaaatctgct
     6961 gcgccgcttt gtgcagtacc gccgcagcca tgaactgcgc cagccgctgg atgatgccgg
     7021 agcatggcaa atgtacagcc attattacga agaactggcc tccgatcgcc agcaggggtt
     7081 tgagatgctg tttaacgagg tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca
     7141 gcgggagccg gaagcatgaa caggaaggcg tttctggcct gcgtgctgat gtgcgtcata
     7201 ttaaccggct gcgaaacggc gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag
     7261 gtcgggagcc tgaaggagca accttcggaa ataaccgtca ccctgctgac cgaaccggac
     7321 accaatacca atgcggaagg tgaatcagcc gccgtggatg tgcaactggt gtacctgact
     7381 gacgactcaa aattgctggc agccgactat gaccagatag ccagtacgcc gctaccggat
     7441 gtgctgggta aaaactatat cgaccaccag gatttcaacc tgttgccgga caccatcaaa
     7501 accctgccgc cggtaaagct ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc
     7561 tccgatgacc aggccactga atggaaacaa atcgagacgg tggaaggaac cggccaccat
     7621 taccgcctgc tggtgcatgt acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa
     7681 ttatggcaac cacccgcaac aaggtgatgt ggcaggaagg gatgctgatg cgcccacacc
     7741 atttccagca acagcagcgt tacaacgact acctggataa ccagcgtttc cgggccatga
     7801 atgatttatc ctggggattt accgaactca ccctcaacaa tgaactgctg gcgcagggta
     7861 agatcatgat tgacagcgcg tcaggcacac tgcccgacgg caccgtcttt tctatccccg
     7921 accaggacgc actgcccgat ccgctgcacc cgcaaaattt tccggacgaa agaagccgca
     7981 atatctacct cgctctgccg gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc
     8041 gaatcgggcg ttaccggctg aattatgccg atgtccggga tttgcattca gaagaaggtg
     8101 acgcgcgaac attgacgctg ggtcaactga cgccgcgcat tatgagcggt gcagaagata
     8161 tgagcgccta tattacgctc ccgctttgcc gtatcagcga tcgccatgcc gatggctccc
     8221 tgacgttgga tgacgatttt atcccctcct gccagaatat tcaggtcagt aagaaactgc
     8281 gtgtttatct caaagaggta cagggggcca ttggcggacg ggcaagcgat ctggcaaacc
     8341 gtattggctc tccggcgcag agcggcatcg cggatgtggc ggaatttatg atgttgcagt
     8401 tacttaaccg taaccagacc cggtttaccc atcgcgcccg ccgatcccag ctccacccgg
     8461 aagatttcta ccttgatctt gctgggttgc tgggtgaact gatgaccttt accgagccgt
     8521 cgcgcctgcc ctgcccgctt gatgtgtatg atcatcatga tctgaccaaa acatttaaaa
     8581 cactgttacc ggaagtcaaa cgggcgctac ataccgtact gtcgccaaga gcggtcaatc
     8641 tgccgctgca tctgcgtgac ggtatctggc aggccgatat ccatgacaca gaactgctgc
     8701 aatctgccac ctttgtgctg gccgtggcgg caaacgtgcc ggtcgatcag atccagcgtc
     8761 agtttatcca gcagtcgaaa atttcctcgc cggaaaaaat ccgcaatatg gtcagtgtgc
     8821 agatccccgg tattccattg cgtgccctta tggtggctcc ccgccagctt ccttatcatt
     8881 ccgggttcag ctatttcgaa ctcgacaaga gcggacaggc ctggacagaa atggctgccg
     8941 ccggggccgt tgcactgcat gtatccggca gtttcccgga tctgaacatg caactgtggg
     9001 cgataagagg gtaagacgat ggatgagggg tcactgtctc tgccaccatt taccggatac
     9061 gacgaaaaaa gccagcgcaa ctaccatctc gcgcttcgtg gcaacagcct gaatccgatg
     9121 atcgacgcgg ccacgccgct actcggtatg gtaatgcgcc tttcaaccat gaacagccag
     9181 acgatgccgg agcatctttt tgctcaggtg gtgacggatg ttcaggccgt ggagcaactg
     9241 cttcaggagc agggctacga accgggcgtg attatctcgt ttcgctacat cccctgcacg
     9301 tttattgatg aagcggcgct cggtaacggc tggtcaaaca agaacgagtg gatcaagcag
     9361 tcattgctgg tccatttcca caacgaagcc tggggtggag agaaagtttt catcctgctg
     9421 gagcgcctga tccgtgaacc caaacgttac caggatctgc tggaatttct ctggatctgt
     9481 ttttctctgg gatttcgcgg gcgctacaaa gtggcagcac aggatcaggg ggaatttgag
     9541 cagatctacc gccgtctgta ccacgttctg cataaactgc gcggcgatgc ccctttcccg
     9601 ctgttacacc aggacaaaaa aacccagggc gggcgttatc agctcatcag tcgcctgact
     9661 gttaaacata ttttttgtgg tggcgttgtc gtgctggcgt tgttttacct gttctatctg
     9721 ttgcggctgg acagccagac gcaggacatc ctgcaccagc taaacaaatt acttcgataa
     9781 ggacgtttat gatccagatt gatcttccca cgctggtaaa acggctgaac ctgttctccc
     9841 gccaggcgct ggagatggcc gcctctgaat gtatgagtca gcaggcagcg gaaattaccg
     9901 tcagccatgt gctcattcag atgctcacca tgccacgcag tgacctgcgg gttattaccc
     9961 gccagggcga tattggcatg gaagagttgc gccaggcgct gacggtagag aactacacaa
    10021 ccgcccgttc tgcggacagc tacccggcgt tttccccgat gctggttgag tggctgaaag
    10081 agggctggct gctggcgtcg gctgagatgc agcacagcga actgcgcggc ggcgtgttgc
    10141 tgctggccct gctgcattcg ccgctgcgtt atataccgcc tgctgccgcc cggctgttga
    10201 ccggcattaa ccgtgaccgt ctgcaacagg actttgtgca gtggacacag gagtcggcgg
    10261 aatcagtcgt gccggatgca gacggtaaag gcgcaggcac aatgacggac gcctctgaca
    10321 ccctgcttgc ccgctatgcc aaaaacatga ccgcagacgc ccgtaacggc aggcttgacc
    10381 cggtactgtg ccgcgaccac gaaatcgacc tgatgatcga cattctctgc cgccgccgta
    10441 aaaacaaccc ggtggtggtg ggcgaagcgg gcgtgggcaa aagcgcactg attgaagggc
    10501 tggcgctgcg catcgtggca ggccaggtgc cggacaagct gaaaaacacc gatatcatga
    10561 cccttgacct gggcgcattg caggccgggg cgtcggtgaa gggtgaattc gaaaaacgtt
    10621 tcaaagggct gatggcggag gtcatttcct ccccggtgcc ggtcattctg tttatcgacg
    10681 aagcacatac cctgattggc gcgggcaacc agcagggcgg gctggatatc tccaacctgc
    10741 tcaaaccggc gctggcgcgc ggcgagctga aaaccatcgc cgccaccacc tggagcgagt
    10801 acaaaaaata cttcgaaaaa gatgccgccc tgtcgcgccg cttccagttg gtgaaggtca
    10861 gcgaacccaa cgctgccgaa gccaccatta ttctgcgcgg tctgtcggcg gtctatgaac
    10921 agtctcacgg cgtgctgatt gatgatgacg ccttgcaggc cgctgcgaca ttaagcgagc
    10981 gttatctctc cgggcgtcag ttaccggaca aagcgattga tgtgctggat accgcctgcg
    11041 cccgtgtggc catcaacctg tcgtcgccgc cgaagcaaat ctcggcgctg accactctga
    11101 gccaccagca ggaggcggaa attcgccagc ttgagcgcga gcttcgcatc ggactgcgta
    11161 ccgacacatc acggatgacc gaggtgctgg tgcagtatga tgaaacgctg acggcgctgg
    11221 atgaactgga agcggcctgg caccagcagc agacgctggt ccgggagatt attgcgctgc
    11281 gccagcagtt actgggcgtg gcagaggacg atgcggcgcc gttgccggac gcagataccg
    11341 tggaggatac gcagccagag tcagagtcag agtcagagtc agagtcagag tcagaacagg
    11401 ataataccgg tgccgtaccg gctgatgagg ccgacagaga acagccggaa gagaccgctg
    11461 aaacagtttc cccggtacaa cgtctggcac atctcactgc cgaactggac gccctgcata
    11521 acgaccggtt gctggtttcc ccgcacgtcg ataaaaaaca gattgcggcg gtgattgccg
    11581 aatggaccgg cgtgccgctt aaccgcctgt cgcagaatga aatgtcggtc atcaccgacc
    11641 tgccaaaatg gctcggcgac accatcaaag gccaggacct ggcgattgcc agcctgcaca
    11701 aacacctgct gaccgcacgc gccgacctgc gccgtccggg acgcccgctg ggcgcgttcc
    11761 tgctggctgg ccccagcggc gtgggtaaaa ctgaaaccgt cctgcaactg gcagagctgc
    11821 tctacggcgg tcgccagtac ctgaccacca tcaatatgtc cgagttccag gagaaacata
    11881 ccgtctcgcg actgattggt tcgcctccgg gctacgttgg ctacggtgaa ggcggcgtac
    11941 tgaccgaagc gattcgccag aaaccctact cggtagtact gctcgatgaa gtggaaaaag
    12001 cgcacccgga tgtgctcaac ctgttctacc aggcgttcga taagggcgaa atggcagacg
    12061 gtgaaggccg cctgattgac tgcaaaaata tcgtcttctt cctgacgtcc aacctcggct
    12121 accaggtaat agtcgagcat gccgatgacc cggaaaccat gcaggaagta ctgtatccgg
    12181 tgctggccga cttcttcaaa cctgccctgc tggcgcgtat ggaagtggtg ccgtacctgc
    12241 cgctgtcgaa agagacgctc gccaccatca ttgccgggaa actggcccgc ctggataacg
    12301 tgctgcgcag ccgctttggc gcggaggtga ttatagaacc ggaagtgacg gacgaaatca
    12361 tgagccgcgt cacccgcgcg gaaaacggcg cgaggatgct ggaatcggtc atcgacggca
    12421 atatgctgcc gccgctctcc ctgctgctgt tgcagaaaat ggcggcgaat acggcggttg
    12481 cccggattcg gttgtcggca gtggacggcg catttacggc agacgtggaa gatgctcaga
    12541 acgacgagtc cgtcacaaag gatgaaacgg ttttatgagc aggttgcgcg ttttccggca
    12601 gacaggatgg tttatcgcgg gcctgatgac gggcttaccc gccactgccg cgcccgcaga
    12661 agcatcgtca atggccggtg tggcggtcgc cgtcgccacc acgaccccgc cggatgcgac
    12721 agccacgtta caggccatgc agtcctgtcg gcgggagtcc gcggcgctgg agcggcttga
    12781 ctgctatgac cgcctgctgg caccgctgtc cccgtcaggg tttgacgggg cgctggtcaa
    12841 agccggtttt gtgggcgaag cctggacccg cgccacggaa caggaaaaac accgccaggg
    12901 caacaccaca gagctgctgg tgacgcaggt gccgggggag cgcccgacgg tggtgataac
    12961 caccccggct atcggtcatg ttccgccacg cccggtgctg atgttcagtt gcgtggacaa
    13021 tatcacccgg atgcaggtgg cgctgatgca ccctctggat gtccatgata tcgccgtcac
    13081 tctgaatgcc gacaaccggg cattgcgcag ccactggttt gtgcgcgaga acggcaccct
    13141 gctggagtcc agccgggggt tatccgggat tgatgaaatt aagcaactgt tcggcgcaaa
    13201 aacgctgacg gtggataccg gcgcagacaa tgccgccgga aagctgacct ttaacattga
    13261 cgggctggca agggcgattg ctcccctgcg cgatgcctgc cactgggcgg gagaataaaa
    13321 atatggcaat ggacttacgc gatccgaatg tctggatatc gcacctgctg gaaaacctgc
    13381 cggaagagaa actggcatcg gcgctgaaag atgacaaccc gaactgggag tatatcgacg
    13441 gcgaaatcgt caagctggga tcgctggccc acagtcaact ggatatcccg gaactccagc
    13501 gccgggggct ggtcattctg gcttcggaga gtaaggattt tcgtctgctg gcgcatctgc
    13561 tgagaaccct gcaacacgcc ggtgatcctc atctcgcatt gcgcctgctg gcgctgtatg
    13621 tggagcatta ctgggcggtg gctgcgccgc agaatatggc gcataaaaaa cgctttgcca
    13681 gccaggtcat taaacgcttt gaaacaggta ttgaagtttt ttcacagaac gccgccacag
    13741 cgcagcgcga tgcgctgtca ggggaactgg caaaactggc gcaatgctgg cagtcacata
    13801 acgccccgga actggcacag gctactgatg atctttttgc cctgtaccag cgtgcgttta
    13861 atcgtgcggc accggctcct gtccccactc cggcggcctc cggtagttca ccacagacca
    13921 ccgccacgtc tgaaagcggc gtgacgcaac ccagtgctcc ggctccccaa atcgtcatcg
    13981 acagtcacga cgacaaagcc tggcgcgaca cgctgttaaa agtggcggcc attttatgtg
    14041 aacgccagcc ggattcaccg cagggctacc gtctgcgccg ccatgcgctt tggcagaaca
    14101 tcaccagtac gccccaggcg gaaagcgatg gccgtactcc actggctgcg gtctccgccg
    14161 atatggtggc agattaccat gctcagctcg gtagtgccga tatggcgctg tggcagcagg
    14221 tggagaaaag cgtactgctg gctccttact ggctggatgg tcactgcctt tctgcacaga
    14281 cggcgctgcg tctgggttac aaacaggtgg cagacgccat ccgcgatgag gtcatccgct
    14341 tccttgagcg tctgccccag cttaccgggc tgctgtttaa tgaccacaca ccgtttatca
    14401 gtgagcagac gaaacaatgg ctggcagctt cgcccgacgc caaagttgca ccggttgcgc
    14461 aaatcggtga ggaatcgaag gcagccagag cctgttttgc tgagcagggt ctggaggcgg
    14521 cgctgcgata tctggacatg ctacccgaag gcgatccccg cgatcagttt caccgccagt
    14581 accttgccgc acagttgacg gaagaggcgg ggctggtaca gcttgcgcag cagcagtacc
    14641 ggatgttgtt caggatggga ttgcagatga tggtggcaga ctgggagccg tccctgcttg
    14701 agcagctaga acaaaaattc acggcagaac aataaaagag aacaggagtt tttgtgttca
    14761 gattaccaac accccgacta ctcagcggac tcaaatcagc cctgcgaccg gcgatgcccc
    14821 ggtttaaagt ctctgctttc tggctgctga tactggcgtg gatttttctg ctggtgtgga
    14881 tctggtggaa aggcccgacg tggacgctgt atgaagaaca gtggctcaaa ccactggcga
    14941 gccgctggct ggcaacggcg gcgtggggga ttattgccct gatgtggctt accgtccggg
    15001 tgatgaagcg cctgcaacag cttgaaaaac agcaaaaaca acagcgcgag gaagccattg
    15061 atccgctcag tgtggaactg aacgcccagc agcgttacct cgaccgctgg ctgctgcgcc
    15121 tgcaacgcca tctcgacaac cgccgtttcc tgtggcaatt gccgtggtat atggtcatcg
    15181 gcccggcggg cagtggcaaa acgacactgc tgcgcgaggg gtttccgtca gacattattt
    15241 atgccccgga gggctcgcgc ggcgcagaac aacgcctgta ccttacgccc catgtcggta
    15301 aacaggcggt gatctttgat atcgacggca cactctgcgc tcccgctgat gcggatatcc
    15361 tgcatcgccg tctgtgggaa cataccctcg gctggctgaa ggaaaagcgc gcgcgccagc
    15421 cgctgaacgg gattattctg acactcgatt tacccgatct gctgaccgca gacaagcgcc
    15481 gccgtgagta tctgctacag acgctgcgca gccgcttgca ggatatacgc cagcatcttc
    15541 actgccagct accggtttac gtggtactga cccggcttga tttattgcag ggttttgccg
    15601 ccctgttcca gtccctgaac agacaggatc gtgatgcgat tctgggtgtc acgttcaccc
    15661 gccgtgccca tgaaaatgat gactggcgaa cagagttgaa tgctttctgg cagacatggg
    15721 tggatcgaat gaatctggcg ttgccggatc tgatggtcgc tcagactcac acccgcgcgt
    15781 ctttattcag tttttcccgc cagatgcagg gaagccgtga acccctggtg tcactgcttg
    15841 aaggtctgct tgacggcgaa aatatgaacg tgatgctgcg tggtgtctat ctcacctcgt
    15901 cactgcaacg cggccagatg gatgacatct ttacccagtc cgccgcccgc cagtaccggc
    15961 tgggcaataa cccactggcg tcctggcccc tggtggacac cgcgccttat ttcacccgca
    16021 gcctgttccc gcaggcatta ctcgcagagc ctaatctggc aacggagagc cgtgactggc
    16081 tgatgcgttc ccgtcgccgc ctgacggttt tctctgccac aggcggcgtg gcggcactgc
    16141 tgctcatcac cggctggcat cactattaca acggtaacta tcagtccggc atcaccgtgc
    16201 ttaagcaggc caaagccttt atggacgtgc cgcctccgca ggggaaagac gactacggca
    16261 acctgcaact gccgctgctg aacccggtac gcgatgccac actggcctat ggcgactggg
    16321 gcgaccgcag ccgtctggcc gatatgggac tgtaccaggg acgacgtatc gggccttatg
    16381 tggaacagac ctatctgcaa ctactagagc agcgttacct gccctcgctg tttaacgggc
    16441 tggtcaaagc gatgaacgcc gcgccgccgg agagtgagga aaaactcgcc gtgctgcgcg
    16501 tgatgcgaat gctggaggac aaaagcggac gcaataatga ggtggtgaag cagtatatgg
    16561 caaaacgctg gagcgaaaag ttccacggcc agcgtgatat ccaggcacaa ctgatgtccc
    16621 atcttgacta cgcgctggct catactgact ggcatgcaga gcgtcaggcg ggcgacggtg
    16681 acgccatcag ccgctggatg ccatatgaca agcccgtggt atcagcacag aaagaactga
    16741 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
    16801 tacctgctga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtctttacat
    16861 ctgccgatga caacaaactg gttgttccac agtttcttac ccgttacggc ctgcaaagct
    16921 attttgtaaa acagcgcgat gaactggttg aactgacggc aatgaattcc tgggtactca
    16981 acctcacccg cagtgtgaaa tacagtgacg ccgaccgcgc ggaaatccag cgccagttga
    17041 ccgagcagta tatcagcgac tacaccgcta cctggcgagc cgggatggac aatctgaata
    17101 tccgcaattt tgagtccatt ggacaactga ccggggcgct ggagcaggtt atcagcggcg
    17161 accagccttt gcagcgggcg ctgactgtgt tgcgtgacaa cacacagcca ggcgtctttt
    17221 ctgaaaaact ttctgccaaa gaacgggatg aggctctggc agaaccggac taccagttgc
    17281 tcacccgcct cgggcatgaa tttgccccgg aaaacagtac tctggcagta cagaaagaca
    17341 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tatctgctgg
    17401 caattcagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttgcggcttg
    17461 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaagaca ctgcctgctc
    17521 cgctcaaccg ctgggttggc agactggctg accaggcctg gcatgtggtg atggtggagg
    17581 ccgttcacta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
    17641 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
    17701 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
    17761 ttatcgacaa tgacctgagt ctggaggatg gcgataacag cgtcattatt cgcgaagata
    17821 ttattgcgca actgaaaacc gcgcagaaaa tccgtgacat cttcttcagt aaacagaacg
    17881 gtctgggaac atcctttgcc gtggaaacgg tatcgctctc cggcaacaaa cgccgcagtg
    17941 tactgaacct tgacggccag ttagtcgatt acagccaggg gcgcaactat accgcccatc
    18001 tggtctggcc taacaatatg cgcgaaggca acgaaagcaa actgacgctc atcggcacca
    18061 gcggcaacgc accgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgccttttcg
    18121 gggccggaca actgaccgga gttcaggatg gcaactttac cgtgcgcttt agcgtggacg
    18181 ctggcgcgat gacctaccgt gtgcataccg acactgagga taacccattc agcggtgggt
    18241 tattcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat ggatagtaac
    18301 ggactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagtgcc
    18361 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
    18421 gtggagtcgc tggcactggc gatttttaaa gccaacggtg tggatttaca caccgccacc
    18481 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
    18541 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
    18601 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
    18661 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
    18721 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
    18781 tttatgcaga acacgcgtaa acggcttgaa ccccagctga agagtaacac tgagaacgcc
    18841 gcacagacca cggtcagaac gctgatttat gccccggaaa cacaggcatc ttccacacca
    18901 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
    18961 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
    19021 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
    19081 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
    19141 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
    19201 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
    19261 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
    19321 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
    19381 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
    19441 caggcggagc ggggactggg gcaggaaaca cccgttgagt ctctgttgac gcagtaccag
    19501 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
    19561 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
    19621 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
    19681 atttatcttt gagtggagag acacaagggc tgatttcagc aggttgctca acgcttgatt
    19741 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
    19801 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
    19861 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
    19921 aatttatact ctaccgaacc agtaaagcg
//
LOCUS       DAZFYC010000088        19764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFYC010000088 DAZFYC010000000
VERSION     DAZFYC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.088"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..163
                     /locus_tag="ACHKW5_003499"
     CDS             <1..163
                     /locus_tag="ACHKW5_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512833.1"
                     /translation="EELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYT
                     YNYDLLNAI"
     gene            complement(558..934)
                     /gene="rnpB"
                     /locus_tag="ACHKW5_003500"
     ncRNA           complement(558..934)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKW5_003500"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(967..2112)
                     /gene="garK"
                     /locus_tag="ACHKW5_003501"
     CDS             complement(967..2112)
                     /gene="garK"
                     /locus_tag="ACHKW5_003501"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO7512834.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2209..3099)
                     /gene="garR"
                     /locus_tag="ACHKW5_003502"
     CDS             complement(2209..3099)
                     /gene="garR"
                     /locus_tag="ACHKW5_003502"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7512835.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3129..3899)
                     /gene="garL"
                     /locus_tag="ACHKW5_003503"
     CDS             complement(3129..3899)
                     /gene="garL"
                     /locus_tag="ACHKW5_003503"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO7512836.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3915..5249)
                     /gene="garP"
                     /locus_tag="ACHKW5_003504"
     CDS             complement(3915..5249)
                     /gene="garP"
                     /locus_tag="ACHKW5_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO7512837.1"
                     /translation="MILDTVDEKKKDVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAHLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5624..7195
                     /gene="garD"
                     /locus_tag="ACHKW5_003505"
     CDS             5624..7195
                     /gene="garD"
                     /locus_tag="ACHKW5_003505"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO7512838.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7344..7679
                     /gene="prlF"
                     /locus_tag="ACHKW5_003506"
     CDS             7344..7679
                     /gene="prlF"
                     /locus_tag="ACHKW5_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO7512839.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7679..8143
                     /gene="yhaV"
                     /locus_tag="ACHKW5_003507"
     CDS             7679..8143
                     /gene="yhaV"
                     /locus_tag="ACHKW5_003507"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO7512840.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8198..9007)
                     /gene="agaR"
                     /locus_tag="ACHKW5_003508"
     CDS             complement(8198..9007)
                     /gene="agaR"
                     /locus_tag="ACHKW5_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO7512841.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9256..10536
                     /gene="kbaZ"
                     /locus_tag="ACHKW5_003509"
     CDS             9256..10536
                     /gene="kbaZ"
                     /locus_tag="ACHKW5_003509"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO7512842.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYTSANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARAMLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10559..11032
                     /gene="agaV"
                     /locus_tag="ACHKW5_003510"
     CDS             10559..11032
                     /gene="agaV"
                     /locus_tag="ACHKW5_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO7512843.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11043..11822
                     /gene="agaW"
                     /locus_tag="ACHKW5_003511"
     CDS             11043..11822
                     /gene="agaW"
                     /locus_tag="ACHKW5_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO7512844.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11812..12690
                     /gene="agaE"
                     /locus_tag="ACHKW5_003512"
     CDS             11812..12690
                     /gene="agaE"
                     /locus_tag="ACHKW5_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO7512845.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12708..13142
                     /gene="agaF"
                     /locus_tag="ACHKW5_003513"
     CDS             12708..13142
                     /gene="agaF"
                     /locus_tag="ACHKW5_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO7512846.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13139..14272
                     /gene="nagA"
                     /locus_tag="ACHKW5_003514"
     CDS             13139..14272
                     /gene="nagA"
                     /locus_tag="ACHKW5_003514"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7512847.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAQPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGNTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14623..15777
                     /gene="agaS"
                     /locus_tag="ACHKW5_003515"
     CDS             14623..15777
                     /gene="agaS"
                     /locus_tag="ACHKW5_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO7512848.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15790..16650
                     /gene="kbaY"
                     /locus_tag="ACHKW5_003516"
     CDS             15790..16650
                     /gene="kbaY"
                     /locus_tag="ACHKW5_003516"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO7512849.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16817..17293
                     /gene="agaB"
                     /locus_tag="ACHKW5_003517"
     CDS             16817..17293
                     /gene="agaB"
                     /locus_tag="ACHKW5_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO7512850.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17332..18135
                     /gene="agaC"
                     /locus_tag="ACHKW5_003518"
     CDS             17332..18135
                     /gene="agaC"
                     /locus_tag="ACHKW5_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO7512851.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAAIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18125..18916
                     /gene="agaD"
                     /locus_tag="ACHKW5_003519"
     CDS             18125..18916
                     /gene="agaD"
                     /locus_tag="ACHKW5_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO7512852.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18917..19672
                     /gene="agaI"
                     /locus_tag="ACHKW5_003520"
     CDS             18917..19672
                     /gene="agaI"
                     /locus_tag="ACHKW5_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO7512853.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQQLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4817 a         5133 c         5012 g         4802 t
ORIGIN      
        1 agaagagtta ctttcagata acaccctcga gaaagacgaa aattctccgg acaatggttt
       61 tgaggtcggg gaatataaca catatgaagc atataactca gagaatcaat attttaccag
      121 agaggactat acgtataatt acgacctttt aaatgcaata tagacttaat aacttcttac
      181 aaagtctatt attaataccg tagatattta ttcatcttac cctgtgcacc aaaagcaaaa
      241 actcactgac gcgggtttga aactgatggc aaccgcaaaa atgcctgatg cgctacgctt
      301 atcaggccta cgccatctct gcaatatatt gaatttgcgt gcttttgtag gcaggataag
      361 gcgttcacgc cgcatccggc atgaataaag cgcactcaac aatctgaaac ccgccggaac
      421 ggtttattac gtacatcagg tgaaactgac cgataagccg ctttcttttg ggtatagcgt
      481 cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccgg tttacgctgg
      541 cgggttttcg ttaaatcagg tgaaactgac cgataagccg ggttctgtcg tggacagtca
      601 ttcatctagg ccagcaatcg ctcactggct caagcagcct acccgggttc agtacgggcc
      661 gtaccttatg aacccctatt tggccttgct ccgggtggag tttaccgtgc cacggactgt
      721 taccagccgc gcggtgcgct cttaccgcac cctttcaccc ttacctgatc ccgcttgcgc
      781 gggccatcgg cggtttgctc tctgttgcac tggtcgtggg tttccccccc aggcgttacc
      841 tggcaccctg ccctatggag cccggacttt cctcccctcc gcccgtctcc cccgaagagg
      901 acgacgacga agcggcgact gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg
      961 cccttgtcac cccgcgttgc gcattccaat cgccagtgtc gcggcgatat tacgtgaagc
     1021 acggcagata ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccta tgctggtcaa
     1081 tacgctgaag accgcatcga tgccatgctg atgtacaacg ccaacatcat cggtcaggct
     1141 acccgcaatg ccaatcaccg gtttatggta cttcttcgcc acgtttgcaa cgccaatcgg
     1201 taccttgccg tgaatactct ggctgtcaat acgcccttca ccggtgatca ccagcgtaca
     1261 atcgtgaata tgttcctcca gattcagcgc cgtagtgacg atttcaatac cacttttcag
     1321 ttccgcacca agaaacgcca tcagcgccgc acccataccg cccgcagctc ctgcgccggg
     1381 gacatctttc acatcaacat gcagcgcttt tttaatgacc tcggcatagt gagagaggtt
     1441 attgtccagc tcaacaatca tcgcttcact ggctcccttt tgtgggccaa agatgcgcga
     1501 tgcgccgtta tcgcccacca gcggattggt gacatcacaa gcgacgcgaa tgacgcaatc
     1561 ttttaagcgc ggatcgaggc cggaaatatc aatatcattc agagtattaa gactaccgcc
     1621 gccaaaacca atttcattgc cgttggcgtc gcataatttc gcccccagcg cctgtaccat
     1681 gcctgcgccg ccatcatttg tagcgctacc gccaatgccg ataataatgt ttgtcgcgcc
     1741 gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt gaagtggtca cgagtggatc
     1801 gcgtttttcc gcaggtacca gctccagccc actggccgcc gccatttcaa taaacgcggt
     1861 tttgccatcg ccggagatcc cccaactggc attcactttc tcgcccagcg gccctgtaac
     1921 ccaggcgtga cgttcagaac cctgggtggc tgcaatcatc gcttccaccg ttccttcgcc
     1981 accgtcggca accggaatag aaacgtactg tgcatcagga aaaatttccc gaaatccttt
     2041 ttctatcgcc tgcgcaacct cgctggcaga taaactttct ttataagagt ctggggcgat
     2101 tacgattttc atacctatgc ctgttaccac atgacgccgg agggcgtttc tcttattcgg
     2161 cctggattcc aggcccggat tgcaatacgc catccgggca cgacgtcatt aacgagtaac
     2221 ttcgactttc gccagttttt cgtagtagca cgccagggcg ctatgatccg ccgttcctaa
     2281 accatctgct cgcagtgcct gcatcatctc cataaccgca gctgtgagcg gcagttgcgc
     2341 gccgacgccg tgggaagtat ccagcgcatt tgccagatcc ttaatatgca gatcaatacg
     2401 gaagcccggc ttgaagttgc ggtccatcac catcggcgct ttggcatcca gcacggtact
     2461 gcccgccagt ccaccgcgaa ttgcctgata caccaggtcc gggttaacgc ccgctttggt
     2521 tgccagcgtt agcgcttctg acatcgcggc aatattcagc gccacaatga cctgatttgc
     2581 cagtttggtg acgttacctg caccgatttc cccggtatgc accacggaac ccgccatcgc
     2641 tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg ccgcccacca tcactgacag
     2701 cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga gcatccagca tatcaatgcc
     2761 tttcgctttc agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag aactcatatc
     2821 gatcaatacc gtacctggct tcgcgccttc aatgatgcca ttctcaccca gcgccacctc
     2881 tttcacatga ggggagtttg gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc
     2941 tttagccgta gacgctgttt ctgcacctgc agcaatcacg tcagcaatag cttctgggtt
     3001 acggtcagca accaccagcg agtaacctgc tttcagaagg tttttactca ttggtttacc
     3061 cataatcccc aggccaataa aaccaacttt catagtcata tcaatcatct ctcttgttgc
     3121 ggtggtggtt attttttaaa ggtatcagcc agtttctgag tggcagagcg gaagacgcca
     3181 agatcgctgc cgacagccac aaacgtcgcg ccccattcca gataacgacg cgcatcggct
     3241 tcgaccggcg cgagaatacc gctgggtttg ccgtgcgcac tggcacggtt aaaaatgtgc
     3301 tgaattgctt tttgtacatc cgggtgcgag gcattgccga gatggcctaa tgccgcagcc
     3361 agatcgctgg ggccgacgaa gataccgtct acaccttcgg tagcggcaat ggcatcgacg
     3421 ttatctacgc cctgctgact ttctatctgg accagaatgg tgatgttctt gttcgactga
     3481 gcgaaataat ccgccacggt gccaaacata ttggcgcggt gagaaacgga gacgccgcga
     3541 atgccttccg gtgggtaacg ggttgatgcc accgcctgct ctgcttcctc ttttgtttct
     3601 acaaaaggaa tcaggaagtt atagaaaccg atatccagaa gacgcttaat aattaccggc
     3661 tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc ctttcagggc cattaactgc
     3721 ggaataaacg tggagatatc gtttggcgca tgttcgccat ccagcaccag ccagtcaaac
     3781 ccagccaaac caagaacttc agtgctaatc gggttagaga gtgctgacca gcaaccaatt
     3841 tgtacctgtt tcgcagccag tgcggctttg aatttattcg ggaaaacatc gttattcatc
     3901 gcttatacct ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac tacgaagagg
     3961 tagcagacca tcgccatcag cgctgaacat cccacgaaaa ccagtgctgc attgaaggag
     4021 tgcagttcac ttaccaggta gccaatcacc agtggagtga caatggaggc aacattgcca
     4081 aagacgttaa agacgccgcc acagaggcca acaatctctt tcggcgcggt gtcagaaatc
     4141 accggccagc ccagagcacc aaatcctttg ccaaaaaaag ccagcgccat tagcatgacc
     4201 accagcgtgg tgttgttggt gtagttacat aagatgatgg tggaagccag caacattccc
     4261 agcacaatcg gtagcttacg tgccagggtc agggataaac cgcgtttgat cagataatcc
     4321 gagaagacac ctcccagcac gccgcccgca aaaccacaca gtgccggaat cgaggcgacc
     4381 agacccactt tcagaatcga catgcctttt tcctgcacca gataaatcgg gaaccaggtg
     4441 aggaagaacc aggtgatggt gttgataaaa tattgtccga aaaatacgcc caacatcatg
     4501 cggttagaga gcaattgctt gatgtaatgc agtttgggac cgcttgctgc cgcactgccc
     4561 ggctttttgt ggtccatatc gaccaccgcg ccattttcag agataaactt cagctcttcc
     4621 gcagacatac gtgggtgatc tgtcgggtta tgaatcaatt tgatccacag cgccgtcagc
     4681 acaaaaccaa tcacccccat aacggtaaag acgtgctccc agccccaggc gaaagtcagc
     4741 cagccaagca gaggcgaaaa gagcgccagc gagaaatatt gcgccgagtt aaagatggcg
     4801 gaggcagtac cacgttcttt cgtcgggaac caggcggcga caatacgggc gttcgccggg
     4861 aatgatggcg cttccgagaa gccgagcata aagcgcataa agaacatgga gatccctgcc
     4921 caggccagcg ggaacatatc aacaaagcct tgcaggaaag tgaatagcga ccagaaaaag
     4981 aggctatagg tataaacttt tttcgagcca aacttatcaa gcagccaacc gccggggatt
     5041 tgcatcagca agtgggccca gccaaaagca gaaaagatgt aacccatcga aaccgcactt
     5101 aactgcaact cttttgccac ttcggtacca gcaatagaca gcgttgcacg atcggcgtag
     5161 ttaacggcgg taacaataaa aataatcagt aatattaaat agcgggtatg cacgtctttc
     5221 tttttttcgt caacggtgtc cagaatcatt ttatttacct cgggtactta tgctgatttt
     5281 tattattatg gggaaggtgt tatttatgag tttcatttat gccgtaacgg caatgaactc
     5341 gggaattagt ataagcagcg cgagaataat aatcattgtg caaatgctaa tttaattaat
     5401 gctatttaaa tattattttg agcatatgca cataaggttg cgcgctaaag cacagatttg
     5461 cgctttacct tgccgggcgg cactgcaatc cctgaaatga ttgacattga tcacatttct
     5521 gcgtttaaac tcctgacatt cttatttcac ccaatgaagt catttatttt taaatgagac
     5581 caggtcctca ttttaataac ccctggctgg agaatatagt acaatggcca acatcgaaat
     5641 cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata atgtggcaat
     5701 tattgttaat gataatggcc tgaaagcagg aacgcgtttt ccggatggac tggaattaat
     5761 tgaacatatt ccccaggggc ataaagtcgc attgctggac attccggcta atggtgaaat
     5821 tattcgttat ggtgaagtga ttggttacgc cgtgcgtgca atcccacgcg gaagctggat
     5881 cgacgaatca atggttgtac taccggaagc gccgccgtta cacacgctgc cactggcaac
     5941 caaagtcccg gcccccttac cgccgctgga aggatacacc tttgagggct atcgcaatgc
     6001 cgatggcagc gtgggcacca aaaacctgct cggcatcacc accagcgtcc actgtgtggc
     6061 aggcgtggtg gactatgtag taaaaatcat tgaacgcgat ctgctaccga aatacccgaa
     6121 cgtcgatggc gtggtggggc tgaatcattt gtacggctgt ggcgtggcga ttaacgcacc
     6181 ggcggcagtt gtgcctattc gtaccatcca caatatttcg ctgaacccta actttggcgg
     6241 cgaagtaatg gtgattggtc tgggttgtga aaagttgcag cctgagcgcc tgctggtcgg
     6301 cacggatgat gtgcaagcta ttccagtaga aagcgccagc attgtcagtt tgcaggatga
     6361 aaagcatgtc ggttttcagt ccatggtcga ggatattttg caggtcgccg aacgccatct
     6421 acaaaaactg aatcaacgtc agcgagaaac ctgtccggct tcagaactgg ttgtcggcat
     6481 gcagtgcggt ggcagcgatg ccttttctgg cgtaacggca aacccggcgg ttggctatgc
     6541 gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttc tcagaagtca cggaagtgcg
     6601 tgacgcgatc catctgctga caccacgcgc agtgaacgaa gaggtcggca aacggctgct
     6661 ggaggagatg gagtggtacg ataactatct caatatggga aaaaccgacc gcagcgccaa
     6721 cccttcgccg ggcaacaaga aaggcggtct ggcaaacgtg gtagagaagg cactcggctc
     6781 cattgctaaa tcgggtaaaa gcgcaattgt tgaagtgctg tcgcccggtc aacgcccgac
     6841 taaacgcgga ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca
     6901 ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct
     6961 gatggcggta cccgtcatta aaatggcgac ccgcaccgag ctggcgaacc gctggtttga
     7021 tttaatggat attaacgcgg gcactatcgc caccggcgaa gaaaccattg aagaggtggg
     7081 ctggaagttg ttccacttta ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga
     7141 tcaatggggg ctgcataacc agctggcagt gtttaacccg gcaccggtga cctgatttct
     7201 tttggaacgt ccctcgcaaa acatggcctt agtgccatgt ttttattgtt taaagccccc
     7261 acgtccatta ataatgcatt tgcattacct tagttcaagc ttataatttg agcagaaaac
     7321 aggctgtaaa aggacagtga atcatgcccg ctaatgctcg ctctcacgct gtactgacca
     7381 ctgaatcaaa ggtcacgata cgcggacaaa caactatccc cgcgccagtg cgtgaggcct
     7441 taaaactgaa gccaggccag gacagcattc attacgaaat tctgcctggt gggcaagtct
     7501 ttatgtgccg actgggagat gaacaggagg atcatactat gaatgcattt ttgcgttttc
     7561 tggatgcaga tatccagaac aacccgcaaa aaactcgtcc attcaacatt caacaaggaa
     7621 agaaacttgt cgctggcatg gacgtcaaca ttgatgatga gattggcgac gacgaataat
     7681 ggattttcca caaagggtta atggttgggc gctatatgct catccctgtt ttcaggaaac
     7741 ctacgacgct ttagttgccg aagtcgagac attaaaggga aaagatcctg aaaattatca
     7801 gagaaaagcc gccacaaagt tattggcggt agtccataaa gtgattgagg agcatatcac
     7861 ggtcaatcca tcatcaccgg cattccgtca tggcaagtcg ttaggctctg ggaaaaataa
     7921 agactggtca cgggtaaaat ttggtgctgg tcgttatcgt ctcttctttc gttatagtga
     7981 aaaagagaaa gtcatcattc tgggatggat gaacgatgaa aacactctgc gcacctacgg
     8041 taaaaaaaca gatgcctata ccgtattcag caaaatgtta aaaagaggac atcctcctgc
     8101 cgactgggaa agcctcaccc aagagacaga agaaaaccat tgatggtgtt cacaatgccc
     8161 gccgaacgta ttcgtgttga ccagcgggca ccctttctca ctccccgacc agaatcactt
     8221 caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca tcaacaatga
     8281 tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg aatttactgg
     8341 aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggttt aaacgggctt
     8401 catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca ccaagaaaca
     8461 gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa aaagattgcg
     8521 actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct tccagcaacg
     8581 cattagccac gttcataccg ttggtcatcg caattacgtc agtgtgctta cgcatcagac
     8641 gagcaatctc aaaagtggta gtcccggaat cgaggatcac ccgatgacca ggctgaatca
     8701 actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagcgcg cttttatctt
     8761 ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca ccataggcac
     8821 gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc acagtagata
     8881 cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc agacgctgaa
     8941 tgatctgttc tcgtcgctcg ctggtgccag tcactcgctt ctcacctgaa gcgtcggtat
     9001 tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct attaacgcct
     9061 ttctattaag caaatgcaag cccaccttgc ccattgacgc aagctactct cgtttcagtg
     9121 actttcatta tgtttctttt gtgaatcaga tcagaaaact attatctttc gttttatttt
     9181 tatctcacca tgacgcagta tcaactgaaa caaaacgaaa gattaatatc gcagtaatct
     9241 gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa agcgggcaaa
     9301 acaaatggaa tttatgccgt ttgttccgca catccgctgg tgctggaggc tgcaatccgc
     9361 tacaccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca ggtagaccag
     9421 ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg tcagctcgcc
     9481 gactcgttga atttcccgca ggatgcgttg attctgggtg gtgaccatct ggggccaaac
     9541 cgctggcaaa acctgccggc cgctcaggca atggccaatg ccgatgattt gattaaaagc
     9601 tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg tcaggacgat
     9661 ccgattccct taactgatga catcgtggct gaacgcgccg cccgtctggc gaaagtggcg
     9721 gaagaaacct gtcttgaaca ctttggcgaa gccgatctgg agtatgtcat tggtaccgaa
     9781 gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac cacgccggat
     9841 gccgcccgcg ccatgctgga agcccatcgt cacgcctttg aaaagcaagg tttgaatgcc
     9901 atctggccac gcatcattgc cctggtggtt caacccggcg tcgaattcga tcacaccaac
     9961 gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga aaactacgaa
    10021 acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatcgct gcgccagctg
    10081 gtgattgacc actttgccat tctgaaagtt ggcccagcgc tgaccttcgc cctgcgtgaa
    10141 gctctgttct ctctggcggc gattgaagaa gaactggtgc cagcgaaagc ctgttctggt
    10201 ctgcgtcagg tgctggaaga cgtgatgctc gaccgcccgg aatactggca aagccactac
    10261 cacggtgacg gcaacgcgcg tcgtctggcg cgtggttata gctactcgga tcgcgtgcgc
    10321 tattactggc cggacagcca gattgatgac gctttcgctc atctggtacg taatctggcg
    10381 gattcaccaa ttccgctgcc gctgatcagc cagtatctgc cgctgcagta cgtgaaagtt
    10441 cgctccggcg agctgcagcc aacgccacgg gaactcatta tcaaccatat tcaggacatc
    10501 ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg aacacgctat
    10561 gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg tcggcgttca
    10621 atgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg ttgccgaaga
    10681 tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg tacgtttctg
    10741 gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga aaatcctgct
    10801 ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg
    10861 cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca aaacggtttc
    10921 tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg tggaatgctt
    10981 cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt gagggattca
    11041 tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt atcgctggcc
    11101 tggatatgtt taacggccta acacatatgc accgcccggt ggtcctcggc ccgttggtcg
    11161 ggctggtact tggcgatctg cataccggaa ttttaaccgg cggtacgctg gaactggtgt
    11221 ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc ggtactatcg
    11281 tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc ggtgtcgccg
    11341 tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg atgtctggcg
    11401 tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt gaacgcgtga
    11461 actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc ctgcctatct
    11521 acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga ttaatcgacg
    11581 gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg ctgaaaatca
    11641 tgatgaaaaa cgtctacatc ccctacttca tcctgggctt tgttgccgca gcctggctca
    11701 agttaccggt gctggctatt gctgccgctg cgctggcgat ggcgctgatc gacctgctgc
    11761 gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa gatggcatct
    11821 aatcaaacca ccctgccgaa cgtcagtgaa aacgaagaaa cattgctgac tggcgtcaat
    11881 gaaaacgtgt atgaagatca gagcattggc gcggagctga cgaaaaaaga tatcaaccgc
    11941 gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat gcaggcttct
    12001 ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa acgcgacctg
    12061 gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct ggtgacattt
    12121 gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag tattcagagc
    12181 accaaaattg ccgtcggtgc gccgctcggc gggattggcg atgcaatgtt ctggctaacg
    12241 ctactgccga tttgtggcgg gataggcgca agccttgcgc tacaaggctc tattcttggc
    12301 gcagtcgtat ttattgtgct gttcaacgtg gtacacctgg ggctgcgttt tggtctggcg
    12361 cattatgctt accgcatggg cgtggcggcg attccactca ttaaagcgaa taccaaaaaa
    12421 gtcggccatg ctgcgtctat cgttgggatg acggtaatcg gcgcgctggt ggcaacctat
    12481 gttcgtttaa gcaccacgct ggaaatcaca gcgggcgacg cagtggttaa gttacagact
    12541 gatgttattg acaaactgat gcctgccttc ttaccgctgg tctacacctt gaccatgttc
    12601 tggctggtac gccgcggctg gagtccgctg cgcctgattg cagttaccgt ggttctcggc
    12661 atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg ttaagtatta
    12721 ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag caaatcctcg
    12781 gcgagcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcacc gcgctgctta
    12841 catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt gtttttctga
    12901 ctgatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg
    12961 gctgtgaagt gatcaccggc accaatttac aactgctact ggagatggtg ctggagcgcg
    13021 aagggttaag cggtgaagag ttccgtgtgc aggcgctgga gtgtggacat cgtgggctga
    13081 ccagtctggt agacgagtta ggccgctgcc atgaagaatg tccggtcgag gaaggaatat
    13141 gacacccgtt ctgcgcgcca gaaggctgct gactgaagag ggatggctcg atgaccatca
    13201 gttgcgtatt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg gcgtgactga
    13261 acgcgatgcg gaactgctct gccctgctta catcgacacc catgtacacg ggggtgcggg
    13321 cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc acaaggcacg
    13381 cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata ccattcatgc
    13441 ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc aagtgctggg
    13501 gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcaac cgccggaatt
    13561 gtttcgcgag cttgaaattg ccgagctgga tcaattgatt gccgtttctc agcacacctt
    13621 acgcgtggta gcgctggcac cggaaaaaga gggggcattg caggccatcc gccatcttaa
    13681 acagcaaaat gtacgagtga tgctggggca tagcgcggcg acctggcaac aaactcgcgc
    13741 cgcgtttgat gctggtgccg acggcctggt gcattgctat aacgggatga caggtttaca
    13801 tcaccgcgaa ccgggaatgg ttggcgcggg attaacggac aagcgcgcct ggctggaact
    13861 gatagccgat ggtcatcatg tgcatccggc ggcaatgtcg ctgtgttgtt gctgtgcgaa
    13921 agagagaatc gtactgatca ccgacgcgat gcaggcagct gggatgccgg atggtcgcta
    13981 tacgttatgt ggtgaagaag tgcagatgca cggtggcgtt gtccgtaccg cgtctggtgg
    14041 gctggcgggc aatacgctgt ctgttgatgc ggcagtgcgc aatatggtcg agttgacggg
    14101 cgtaacgcct gcggaagcca tccatatggc gtcgctgcat ccggcgcgaa tgctgggtgt
    14161 tgatggtgtt ctgggatcgc ttaaaccggg caaacgcgcc agcgtcgttg cgctggatag
    14221 cgggctacat gtgcaacaaa tctggattca gggtcaatta gcttcgtttt gatggtttgc
    14281 tccattattg ggccttcact tcccccgtaa ggcctttctt tttctttctt tttgatctgt
    14341 gcagcggtgt cggatgcgac gctaacgcgt cttatgcgac ctacggtacg ccgcatccgg
    14401 caatcggtga acatagcgac gtcattacgc aattctttaa tcttcccttg tcttttcttt
    14461 caacgatcac agatttcgtt ttatttcctt tttcttcatt gaactttctg tttcttttct
    14521 atagatttta atcaacgaaa gacatcacca agtgaaatta aacgaaaggc aagtgaaagc
    14581 gacaacgccc gacgtcaagt tcatcagact aaggattgag ttatgccaga aaattacacc
    14641 cctgctgccg ccgcaaccgg tacatggact gaagaagaga tccgccatca gcctcgcgca
    14701 tggatccgtt cactcaccaa catcgacgcg ctacgttccg cgctcaataa cttccttgaa
    14761 ccgttactgc gcaaagagaa tctgcggatc atcctgaccg gagccggaac ctcggcattt
    14821 atcggtgaca tcatcgcgcc gtggctcgcc agccataccg gtaaaaactt cagcgccgta
    14881 ccgaccaccg atctggtcac taatccgatg gactacctga acccagccca tccgctgctg
    14941 ttgatctcct tcggtcgatc cggcaacagc ccggaaagcg tcgctgccgt ggaactggca
    15001 aatcaatttg taccagaatg ctatcacctg ccgatcacct gcaacgaagc gggcgctctt
    15061 taccaaaacg cgatcaacag cgataacgcg tttgccctgc tgatgcccgc agaaacgcac
    15121 gatcgcggct ttgcgatgac cagcagcatt accaccatga tggccagctg cctcgcggtt
    15181 ttcgcacctg agacgatcaa cagccaaacc ttccgcgacg tggcggatcg ttgccaggcg
    15241 atcctgacct cactgggcga tttcagcgaa ggtgtgtttg gttacgcacc gtggaaacgg
    15301 atcgtttatc tcggcagcgg tggcttacag ggcgcagcac gcgagtcggc gctgaaagtg
    15361 ctggaactga cggcgggtaa actggcggcc ttttatgatt ctccaaccgg attccgtcat
    15421 ggaccaaaat cgctggtcga taacgaaaca ctggtggtgg tatttgtctc cagccaccct
    15481 tacacccgtc agtatgatct tgatctgctg gctgaactcc gccgtgacaa ccaggcaatg
    15541 cgcgtaatcg ccatcgccgc ggaaagcacc gacatcgtcg ctgccggtcc acatattatc
    15601 ctgccgccgt cacgtcactt tatcgacgtt gagcaggcat tttgcttcct gatgtacgcc
    15661 cagacgtttg cactgatgca gtcgctgcac atgggcaata cgccggatac cccatcagcc
    15721 agtggcaccg ttaaccgcgt ggtgcaaggc gtaatcattc atccgtggca ggcataagag
    15781 gatcgcatta tgagcattat ctccactaaa tatctgttac aggacgccca ggccaatggc
    15841 tacgcggtgc ctgcttttaa cattcataac gccgagacga tccaggcgat cctcgaagtg
    15901 tgcagtgaaa tgcgatcgcc ggtgatcctc gccggaacgc cggggacctt taaacatatc
    15961 gcgctggaag agatctacgc cctgtgcagc gcctattcca caacctacaa catgccactg
    16021 gcgctgcatc tcgaccacca cgaatcgctg gatgatattc gccgtaaagt ccacgcaggt
    16081 gtgcgcagtg caatgatcga cggcagccac ttcccgtttg ccgagaacgt gaagctggtg
    16141 aaatcggttg ttgacttctg ccactcacag gattgcagcg tggaagcaga actgggccgc
    16201 ctgggcggtg ttgaagatga catgagcgtt gacgccgaaa gtgcattcct gaccgatcca
    16261 caagaagcta aacgctttgt cgaactgact ggcgtcgaca gcctagcggt agcgattggt
    16321 acggcgcacg gcttatacag caaaacgccg aagattgatt tccagcggct ggcggaaatt
    16381 cgtgaagtgg tggatgttcc tctggtgcta catggtgcca gcgatgttcc ggatgaattt
    16441 gtccgtcgca ctattgaact tggcgtcaca aaagtgaacg ttgccacaga attaaaaata
    16501 gccttcgctg gcgcggttaa agcctggttt gcggaaaatc cgcagggtaa tgatcctcgt
    16561 tattatatgc gcgtcggaat ggatgcgatg aaagaagttg tcagaaataa aattaatgtc
    16621 tgtggttcag cgaatcgaat ttcagcataa tcattagttt tttatgattt atcccaatgt
    16681 acttcccgat taatatcggg gagtgcctta atggaaaagg agatgactaa accttaataa
    16741 atacatcagt acaatatcgc aacaataata tattaaaaaa aatcatatta ttcaacttta
    16801 tggtgaggat tacacaatga gcagtccaaa tattctctta acccgtattg ataaccgtct
    16861 ggttcacggt caggttggcg tgacctggac atccaccatc ggtgcgaatc tgctggtagt
    16921 cgtggatgat gttgtcgcta acgatgatat tcaacagaaa ttaatgggta ttaccgcaga
    16981 aacctacggc tttggcattc gtttctttac tatcgaaaaa accattaacg tcatcggcaa
    17041 agctgcaccg catcagaaga tcttcctgat ttgccgtacg ccacaaacgg tacgtaaatt
    17101 ggtagaaggt ggcattgacc tgaaagatat caacgtcggc aatatgcatt tctcggaagg
    17161 gaaaaagcaa atcagcagta aagtttatgt cgatgaccag gatctcgcgg acttacgttt
    17221 tattaaacaa cgtggcgtga atgttttcat tcaggacgtc cctggcgatc aaaaagaaca
    17281 aatccctgac taaatctaaa atcgccttaa tattggtttg aggtaataaa aatgcatgaa
    17341 ataaccctac ttcagggatt atccctggcg gcgttagttt tcgttctggg gattgatttt
    17401 tggctggaag ccttattttt attccgcccg ataatcgttt gtaccctaac tggcgctatt
    17461 ctcggtgata ttcagactgg cttaattacc ggtggtctga cagagttggc tttcgccgga
    17521 ttaacccctg caggtggtgt tcagccgccc aacccgatta tggcgggtct gatgaccacc
    17581 gtcattgcat ggtctacggg cgttgatgcc aaaacagcaa ttggtcttgg cctgccgttt
    17641 agtttgttaa tgcagtacgt cattctgttc ttctattccg ctttctcatt atttatgacc
    17701 aaagccgata aatgcgcgaa agaggcggat acggcagcgt tttcccggct taactggaca
    17761 acgatgctca tcgtcgcttc agcgtatgcg gcgattgctt tcctctgtac ttacctggca
    17821 caaggggcga tgcaggcgct ggtgaaagcg atgcccgcct ggttgaccca cggctttgaa
    17881 gtggctggcg gtattctgcc tgccgttggt tttggcttgc tgctgcgcgt aatgttcaaa
    17941 gcgcaatata tcccttacct gatcgctggc ttcctgtttg tttgctacat ccaggtcagc
    18001 aacctgttgc cggttgccgt actgggtgca ggctttgcgg tgtatgagtt tttcaatgcg
    18061 aaatcccggc agcaagcgca accgcagccc gttgccagta aaaatgaaga agaggactac
    18121 agcaatggga tctgaaatca gtaaaaaaga tatcacccgt ctgggctttc gttcgtcgct
    18181 gctgcaagcg agctttaact acgaaaggat gcaggcgggc ggttttacct gggcgatgtt
    18241 gccgatcctg aaaaagattt ataaggacga caaaccgggc ttaagcgcgg cgatgaaaga
    18301 taacctcgaa tttattaata cccacccgaa tctggtcgga ttcctgatgg ggttattaat
    18361 ttcgatggaa gaaaaaggag aaaaccgcga cactattaaa ggcctcaaag tggcactgtt
    18421 tggcccaatt gccgggattg gcgatgcgat tttctggttt actttgttgc cgattatggc
    18481 gggaatttgc tcatcatttg ccagccaggg aaacctgctg gggccgattc tatttttcgc
    18541 cgtttacctg cttatctttt tcctgcgcgt cggctggacc cacgtcggtt attcagtcgg
    18601 cgtgaaggcg atcgataaag tgcgagagaa ctcgcagatg attgctcgct cggcaaccat
    18661 cctcgggatc acggtaatcg gcgggctgat cgcttcgtat gtgcatatta acgtggtgac
    18721 atcgtttgcc atcgacagta cccacagcgt cgcgctgcaa caggatttct tcgataaagt
    18781 cttcccgaac attttaccga tggcctacac cctgctgatg tattacttcc tgcgggtgaa
    18841 aaaagcgcat ccggtgctgt taatcggcgt gacttttgtg ctctctattg tttgttccgc
    18901 attcggcatt ttgtaaatgg aacgaggcac tgcgtctggc ggtgcctctt tacttaagga
    18961 atttcatcct gtgcaaaccc ttcagcaagt tgaaaactat acggcgttaa gtgaacgtgc
    19021 cagcgaatat ttattggccg tgatccgtag caaaccggat gccgtgattt gcctggcgac
    19081 cggagccacg ccattactga cgtatcatta tctggtagaa aaaatccacc agcagcaggt
    19141 tgatgtcagc cagctcacct tcgtgaagct cgacgaatgg gtggatctgc cattaacgat
    19201 gcctggtacc tgcgaaactt tcctgcaaca gcatatcgtg cagcagctgg ggctacgtga
    19261 agaccagctc atcagctttc gctccgaaga gataaatgag acagagtgcg aacgggtaac
    19321 gaacctgatt gcgcgcaaag gcggtctgga tttatgcgtt ctaggattgg ggaaaaacgg
    19381 tcatcttggg ctgaacgaac cgggagaaag cctacaaccg gcctgccata tcagtcaact
    19441 tgatgccaga acacagcaac atgagatgtt aaaaaccacg ggtcgccccg tgactcgtgg
    19501 gatcacctta ggcctgaagg atattctcaa tgcccgcgag gttttgttat tggtgactgg
    19561 cgaaggaaag caggatgcga cagaacgttt tctcacggct aaagtctcca ccgctatccc
    19621 ggcttcgttt ttatggctgc acagtaactt tatttgttta attaatacct aaccagatga
    19681 aaatttgctc aaaattttta aattaactta ttcaacagtc acgcattata ttaaataaca
    19741 tttgactggg ttgaacataa cgcc
//
LOCUS       DAZFYC010000089        19621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFYC010000089 DAZFYC010000000
VERSION     DAZFYC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.089"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            119..1252
                     /gene="ompN"
                     /locus_tag="ACHKW5_003521"
     CDS             119..1252
                     /gene="ompN"
                     /locus_tag="ACHKW5_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO7512854.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1393..1827
                     /gene="uspF"
                     /locus_tag="ACHKW5_003522"
     CDS             1393..1827
                     /gene="uspF"
                     /locus_tag="ACHKW5_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO7512855.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2003..2938
                     /gene="ttcA"
                     /locus_tag="ACHKW5_003523"
     CDS             2003..2938
                     /gene="ttcA"
                     /locus_tag="ACHKW5_003523"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO7512856.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3067..4440)
                     /gene="dbpA"
                     /locus_tag="ACHKW5_003524"
     CDS             complement(3067..4440)
                     /gene="dbpA"
                     /locus_tag="ACHKW5_003524"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO7512857.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4470..4643)
                     /gene="ynaL"
                     /locus_tag="ACHKW5_003525"
     CDS             complement(4470..4643)
                     /gene="ynaL"
                     /locus_tag="ACHKW5_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO7512858.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4918..5901)
                     /gene="zntB"
                     /locus_tag="ACHKW5_003526"
     CDS             complement(4918..5901)
                     /gene="zntB"
                     /locus_tag="ACHKW5_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO7512859.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5915..5962)
                     /gene="ydaN"
                     /locus_tag="ACHKW5_003527"
     CDS             complement(5915..5962)
                     /gene="ydaN"
                     /locus_tag="ACHKW5_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO7512860.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6156..7388
                     /gene="dgcM"
                     /locus_tag="ACHKW5_003528"
     CDS             6156..7388
                     /gene="dgcM"
                     /locus_tag="ACHKW5_003528"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO7512861.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7409..7972)
                     /gene="smrA"
                     /locus_tag="ACHKW5_003529"
     CDS             complement(7409..7972)
                     /gene="smrA"
                     /locus_tag="ACHKW5_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO7512862.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRALQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8302..9210)
                     /gene="abgR"
                     /locus_tag="ACHKW5_003530"
     CDS             complement(8302..9210)
                     /gene="abgR"
                     /locus_tag="ACHKW5_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7512863.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9386..10696
                     /gene="abgA"
                     /locus_tag="ACHKW5_003531"
     CDS             9386..10696
                     /gene="abgA"
                     /locus_tag="ACHKW5_003531"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO7512864.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGM
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEEASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10696..12141
                     /gene="abgB"
                     /locus_tag="ACHKW5_003532"
     CDS             10696..12141
                     /gene="abgB"
                     /locus_tag="ACHKW5_003532"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO7512865.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSARP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12175..13701
                     /gene="abgT"
                     /locus_tag="ACHKW5_003533"
     CDS             12175..13701
                     /gene="abgT"
                     /locus_tag="ACHKW5_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO7512866.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13712..14227
                     /gene="ogt"
                     /locus_tag="ACHKW5_003534"
     CDS             13712..14227
                     /gene="ogt"
                     /locus_tag="ACHKW5_003534"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO7512867.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14422..15174
                     /gene="fnr"
                     /locus_tag="ACHKW5_003535"
     CDS             14422..15174
                     /gene="fnr"
                     /locus_tag="ACHKW5_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO7512868.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15326..16276
                     /gene="uspE"
                     /locus_tag="ACHKW5_003536"
     CDS             15326..16276
                     /gene="uspE"
                     /locus_tag="ACHKW5_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO7512869.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16326..16583)
                     /gene="ynaJ"
                     /locus_tag="ACHKW5_003537"
     CDS             complement(16326..16583)
                     /gene="ynaJ"
                     /locus_tag="ACHKW5_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO7512870.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16827..17858
                     /gene="ynaI"
                     /locus_tag="ACHKW5_003538"
     CDS             16827..17858
                     /gene="ynaI"
                     /locus_tag="ACHKW5_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO7512871.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(17909..19522)
                     /gene="mppA"
                     /locus_tag="ACHKW5_003539"
     CDS             complement(17909..19522)
                     /gene="mppA"
                     /locus_tag="ACHKW5_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO7512872.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         4988 a         4847 c         4727 g         5059 t
ORIGIN      
        1 ggttttactt aaggcgtaac aaatgatatt tatcggctaa ctgaacttct cctttaggat
       61 gttttcactc cccgaaatgg ggataaaagg caaataaaat aactaaagga tttattcaat
      121 gaaaagcaaa gtactggcac ttttaattcc tgccctgctc gccgcaggtg ctgcacatgc
      181 agccgaagtt tataataaag acggcaacaa attagatctg tatggcaaag ttgatggcct
      241 gcattatttt tctgataatt cggcgaaaga tggcgaccag agctatgcgc gtctgggttt
      301 taaaggcgaa acccaaatta acgatcaact cactggctac ggtcaatggg aatacaatat
      361 tcaggcaaac aacactgaat cttcaaaaaa ccagtcatgg acccgtctgg catttgccgg
      421 gctgaaattt gcagattacg gttctttcga ttacggacgt aattatggcg taatgtacga
      481 catcgaaggc tggaccgata tgctgcctga atttggcggt gactcttata ccaatgcaga
      541 caactttatg actggtcgag ccaatggcgt cgcgacttat cgtaatactg atttcttcgg
      601 tctggtaaat ggtctgaact tcgcggtgca gtatcaaggt aacaacgaag gtgccagtaa
      661 tggtcaggaa ggcaccaaca acggacgtga tgttcgccat gaaaacggtg acggctgggg
      721 tctttccaca acatatgatt taggcatggg ctttagcgct ggtgcggcat acacctcttc
      781 tgaccgcacc aatgatcagg ttaaccatac tgcggcgggt ggtgataaag cagacgcgtg
      841 gactgctggg ctaaaatacg atgctaacaa tatttacctg gcaaccatgt attcagaaac
      901 gcgtaatatg accccgtttg gcgacagcga ttatgctgtt gcaaacaaaa cccagaactt
      961 tgaagtcact gcacagtacc agtttgattt tggtctgcgt ccggcagtct ctttcctgat
     1021 gtctaaaggc cgtgacctgc acgctgccgg tggtgcagac aacccggcag gtgttgatga
     1081 taaagatctg gttaaatacg ccgatgttgg cgcgacttac tatttcaata aaaacatgtc
     1141 cacttatgtt gactataaaa tcaacctgtt ggatgaagat gacagtttct acgctgccaa
     1201 tggcatctct accgatgata ttgtcgcttt aggtctggtt tatcagttct aaatcctcct
     1261 gcccgctgtt atggcgggct ttttctgctt attcttcctc ctttgatcta aattaaaaat
     1321 gtgaactccg tcattacaca aaaagtgtca tctggcgtta cactttatgc ggatactaaa
     1381 acaggaggtt ttatgaacag aacgattctt gtccctatcg atatttccga ttcagaatta
     1441 actcaacgcg tgattagcca cgttgaggca gaggcaaaga ttgatgatgc agaggttcat
     1501 ttcctgacgg taataccttc actgccctac tatgcctctc tgggtttagc gtattccgca
     1561 gaattaccgg caatggatga cctgaaagcg gaagccaaat cgcaactgga agagatcatt
     1621 aaaaaattta aactgccaac cgacagagtg catgtccatg ttgaggaagg ctcgcccaaa
     1681 gaccgcattc tggaaatggc gaagaagatc cccgctcata tgatcatcat tgcttcccat
     1741 cgaccggata tcaccactta tctgctcggt tccaacgccg cagctgtagt gcgtcacgca
     1801 gagtgctccg tgctggttgt gcgctgacac taacgcccgc acattgctgc gggctttttg
     1861 attcatttcg caaatgtgct gacattttcc cctctaatcc gtaccataca cgccactgtt
     1921 tttatatcag atttcttatg ctgggcattc cggcatgacg catactcttc tgatgccata
     1981 taacgaattg agtcgctttt aaatgtcgca aaatcaagaa attagtaaga aagaacaata
     2041 caacctgaac aaattacaaa aacgtctgcg tcgtaacgtg ggcgaagcca ttgctgactt
     2101 caatatgatt gaagaaggcg atcgcatcat ggtttgcctc tccgggggta aagacagcta
     2161 taccatgctg gagattctgc gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt
     2221 ggctgttaac ctcgatcaaa agcaaccggg cttcccggaa cacgttctgc ccgagtatct
     2281 tgaaaagctg ggcgttgagt acaagattgt tgaagagaat acttacggta tcgtgaaaga
     2341 gaagattcca gagggcaaaa ccacttgctc actgtgttct cgccttcgtc gcggtatcct
     2401 ttatcgtacc gcaacggaac tgggggcgac gaagatcgcg ttgggtcacc atcgtgacga
     2461 tatcctgcaa acgttgttct taaatatgtt ctacggcggc aagatgaaag gtatgcctcc
     2521 gaaactgatg agcgatgatg gcaaacatat cgttattcgt ccgctggcct actgccgcga
     2581 gaaagatatt cagcgatttg ccgatgcaaa agcgttcccg attattccgt gcaacctgtg
     2641 cggttcacag cctaacctgc aacgtcaggt gattgctgac atgttgcgtg actgggataa
     2701 acgttatcca gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca
     2761 tctgtgcgat accaacctgt tcgattttaa aggcattacc cacggttctg aagtggttaa
     2821 cgggggtgat ctggcgtttg atcgcgaaga gatcccacta caaccggcgg gctggcagcc
     2881 agaagaagat gaaaatcagt tggatgagtt acgactgaat gtggttgaag tgaaataacc
     2941 aggatagcgc ccgatgcgca agcgtatcgg gctactctta tggaggccgg ataagacgcg
     3001 gccagcgtcg catccggcaa tcccgaataa gatgtttact cttgcacccg gcaattcaac
     3061 atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa
     3121 ctgtttccat gctttatgag caacagcctg acggaccgcg acatagacat gcgccggatg
     3181 cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca gtgcacctaa
     3241 tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg ttgccatttc
     3301 tgcttccagc ggcacaatgg aactattagc tggcggcgtt tgccagttaa gttttatctg
     3361 caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggagcacaga aactgatcgc
     3421 cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc
     3481 ccacgccagc tcaaagttca ccaccagctc aagcgattta atatccagac cacgcgcagc
     3541 aacatcagta gcaaccagta cacgggcgct accgttagca aaacgtacca gagtctgatc
     3601 gcgatcgcgt tgctccagat cgccgtgtaa cgacaatgca ctttgcccta cttcattcag
     3661 cgcgtcgcag acagcctggc aatctttttt ggtattgcaa aacaccacgc aagaggatgg
     3721 ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttgctgg atgtctcata
     3781 aaattgttgt tcaatgggtg gcaaagcatc tgttgagtca atttcaatcg ccaaaggatc
     3841 gcgttgtact cgtccgctga ttgcggcgat ggcttccggc caggttgccg aaaacagaag
     3901 cgtctgtcga gatgcaggcg caaaacggat gacatcatca atggcatcgc taaatcccat
     3961 atccagcatg cggtcggcct catccatcac caaagtattc agcgcatcca gtgataccgt
     4021 gcctttttgc aagtgatcca gcaaacgacc cggcgttgcc acgataatat gcggcgcatg
     4081 ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca aaattttggt
     4141 atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg ccagttcacg
     4201 cgtaggacac agcactaaag cctgggtttg aaatagcgac gcatcaattt gctgtaacaa
     4261 gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc gaacatcttt
     4321 tccggcaagg atcgccggaa gcgcggcggc ctgcaccggc gtcatggtta aataacccaa
     4381 ctcattaagg ttcgtgagtt gggcgggagg caaaacattc agggtagaaa aagcggtcac
     4441 aatctattct cgtggtcatc gacgcaaagt tagcaggcgc gtatcctcgc agatctacgc
     4501 tcacgatgcg acaatttaat cggttcttca tcaggtggtg ggtcaggcat gggttgcggg
     4561 cgagggatcg gatcgggcac tggaacagga tcgccaggaa tcggttcagg gacaggaatt
     4621 tgcaaataaa taagtgtcgt catatttccc tccggtcatt gggtggactc ttaaagggta
     4681 gacgctgata aataacaggc aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc
     4741 aattgtgcta tgcagtaatt caaaaaagga agtaagacaa tatggagcgc aacgcccatc
     4801 gcttgacgtt gcattcacct gcaagggaga tattgccctg aatgggtaga gagtttattg
     4861 acttcgctca aactttgcgg cgtttttgta tacagacagc cggaaaaatt gcttttgtta
     4921 caaccattta ctacgatgca accataaagc aacaccacca ataagaacaa ctaacagaat
     4981 acaaaaaatt gaaaatccga attgccaccc gccgccaggg atcccaccaa ggttgacgcc
     5041 aaataaccct gtcagaaagg tactgggtaa aaagaccatt gccatcaatg acattgtata
     5101 ggtacgacga gctaaatttt cctgcatcac ctgagcgatt tcatccgcca tcacgccagt
     5161 ccgtgctata caggcgtcga tttcgtcaag accgcgccca aggcgatcgg caatatcctg
     5221 catccgacgg cgttggtcat cgctcatcca cggcaaacgt tcactggcaa gacgagcata
     5281 aacatcacgt tgcggtgcca tatagcgacg catcacgatt aattgtttgc gcagcagagc
     5341 caggaatcca cgcggtggaa tttgctgatc aaggagatta tcttcaaggt cgataatttt
     5401 atcgtgcagc tgctcgataa attcgctgga atgatcggtc aacgcatcgc acacatccac
     5461 cagccaccct ccgcaatcgg tcggacccgt gccctcttcc agatcgctca ccacatcgtc
     5521 cagcgccagc actttgcgtt gtcgggtcga aacaattaac cgcccgtcca tatatacacg
     5581 catggcgacc agttgatcgg ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat
     5641 cagcgtgcct tcaccgagac ggctgactcg gggccgagtg ctctcgcctg ccagcgcatc
     5701 acgtacgtta ttgggaagca gcggtgttgt cgccagccat tgggcgcttt catggtgtac
     5761 ataattaagg tggagccagc agggatgcgc ttcatcaatc acatctgtat tttccagcgg
     5821 tttaacgccg cctctaccat ccagcatcca ggcaaatact gcatccggga cattaacgtc
     5881 cgatccctta atcgcttcca cagtgcctcc atcatcaacg cattattttg tagtctagcc
     5941 ttctggccct gttacgcaac atctcatcac cccattaccc tgaaatgatt aataaaattc
     6001 tgtctaaatt gaatacaaaa agcaaaatgc ttttccgtat acaaaccgtg tgaagtgtta
     6061 aatagcgtct atcattatca gaattatctg accatatgac gtggcttttt tgcgatcgga
     6121 tagcaacaaa aattgataaa aataacggga tctcaatgat tacgcacaac ttcaataccc
     6181 tggacttact caccagtcct gtctggattg tttcgccctt tgaggaacag ttaatttatg
     6241 ccaatagcgc ggcgcgactg ttgatgcaag acctcacgtt tagtcagcta cgaaccggac
     6301 cctattccgt ctcctcacaa aaagaactgc cgaaatacct ctccgatctg caaaaccaac
     6361 acgatattat cgaaatcctc actgttcagc gtaaagaaga ggaaacagca ttaagctgtc
     6421 ggcttgtttt gcgagagctg acagaaacag aaccggtgat tattttcgaa ggtatcgaag
     6481 cgccggcaac gctgggttta aaagccagtc gctcggcaaa ttatcagcgc aaaaaacaag
     6541 gtttttatgc gcgctttttt ctgactaact ctgcaccaat gttgttgatt gacccctcac
     6601 gagatggcca aatcgtcgat gctaacctcg ccgcgctcaa tttctatggt tataaccatg
     6661 aaacgatgtg ccagaaacat acctgggaaa ttaatatgct cgggcgtcgc gtcatgccta
     6721 tcatgcatga aatttcgcat ttacccggcg gtcataaacc tttgaatttt gttcataaac
     6781 tggcggatgg atctactcgt catgtgcaga cctatgccgg gccgattgaa atttatggcg
     6841 acaagctcat gttatgtatt gtgcatgata ttactgagca aaaacggctg gaggagcagc
     6901 tggaacatgc tgctcaccat gacgcgatga ccggattact gaatcggcga cagttttatc
     6961 acattaccga gccaggccaa atgcagcatc tcgccatcgc tcaggattac agcttgttgc
     7021 tcatcgacac cgatcgtttt aaacacatta acgatctcta tgggcattct aaaggtgatg
     7081 aggtgttatg cgccctcgcc cgcacccttg aaagttgcgc tcgcaaaggc gatttggtgt
     7141 ttcgttgggg aggcgaagag tttgtcttat tgctaccaag aaccccactg gataccgcgc
     7201 tttcgctggc tgaaactatc cgtgtaagcg tggcaaaagt gagtatttcg ggcttaccac
     7261 gctttaccgt cagcattggt gtggcgcatc acgaaggaaa tgaaagcatc gatgaactgt
     7321 ttaaacgcgt tgatgatgct ttgtatcggg cgaaaaatga tggacgcaac cgcgtgctgg
     7381 cggcataagc cgcggatgcg tctcgagatc aacgactgcg cttagcgtgg cgctcccagt
     7441 tttcttgctt cgcctgcgcc gttttacgta gtgcgacgta acacgccccg ctgccgccat
     7501 gatgcggtag cgcagtgcaa tatgcctgaa catcatcaaa ttcggtcagc cagcgcgcca
     7561 catagctgcg gacaatattg gcatgcgatt tatcatcccg tcctttgcca tgaataatca
     7621 gcacgttacg caaaccatca gccatggctt gttgaatgaa actgaacatc attttgcggc
     7681 actcttccac cggctggcgc aaaagattca ggctcgcctg ttgcggatat ttaccactgc
     7741 gcagcttatc cagcacccca tgttgcaacc cttcccgccg aaactccagc ggctgactta
     7801 gtgggatgat gtcgagaaat ccggtggtga ggaaattatc aagctgcagc gtgtcgatac
     7861 gctgcagcgc acgttggtta cgcgttggat gccagtggac atcggtagca cgtttcagcg
     7921 gctggacatc ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc atgttacatc
     7981 ctcccgcaat taagagcgcg atatgataac cagaccgggt cggtccaaca acgtattacc
     8041 caaaattcca gtaataagtt ccaaatattg ccgatatttt aagcaaaatg cttatgcatg
     8101 attattcatt cacgatatta ataatgtaac ttatattttc gtgaaatctg tcactgaaga
     8161 aaattggcaa ctaaaggtta aaaccgttat aacacagtca ccggcgcaga ggagacaatg
     8221 ccggacttaa gacgcggatg cactgctgtg tgtactgtag agtctggcgg atgtcgacag
     8281 actctatttt tttatgcagt tttaactttg cagatagccg cattctcgcc ggaattgcgt
     8341 gattaatgac gcggtcagtg gtgtctggcg actatcacgc cgctgaatca aataataggc
     8401 cgctttcggt aaaatttcgc taaccggcaa catcaccagc ccctgtccgt gcaagggatc
     8461 gcagcccatt tcttcaggca gtatgctgag aaaatcactt tttgccacca gactgataca
     8521 ggctgagaac gtctcgcaga ctacaccgac ctgtggcgtt tgcgcctgat cgtcaagcaa
     8581 ttcactcaac tgtttgtagt aactgccgtg tggcgtcggc attgtccagc tgtaatccag
     8641 taactgtttg atcgaacggg caccaatggc ggggtgtccc gggcggcaaa agatcgcgaa
     8701 ttgcttttcc agtaatttct caaaagtaaa ttcgtggtcg tacggtccct gataataggt
     8761 attgatggtg aaatccaatt ctccctgacg caattcatta atcatcgaca ccagttgccc
     8821 ttccataatg cgtactttta cctgcggatg ctgctgatga aaacgagata tgacagctgg
     8881 catcagactg cgggaaatac tggcccccat gccgatatta atctgccctg ccagttgccc
     8941 ttgtcgttgg cgaatttcct cttgcgctgc gcgcagctct tcaagaataa gactggcgtg
     9001 ctgataaaaa ctttcaccgg catcagttaa cgtcacgcct ttactacggc gaaaaaagag
     9061 ttgcgccgct aatccttctt ccagctcctg aatagattta ctcagtgccg gttgcgacat
     9121 attcaacatt cggctcgctc cgcgaatgct gccctgacga gccacttcaa caaaagcacg
     9181 aatttgatga atttttacct gaaaagccat gacgccaccg ataaccgtta tttatcagac
     9241 caaagaaact ggcatctact ttaatgcaga tgattgtgtc agggtaattt atgaacggtt
     9301 aaaactgtga aaaatcagtt agtgataagt aaaaactatc gctacatgaa ccgggtcaca
     9361 ctttttactg atgacgggaa aggttatgga gtctttgaat caatttgtta attcgcttgc
     9421 cccaaaatta tcgcactggc gacgtgattt tcatcactat gcagagtctg gctgggtgga
     9481 attccgcact gccacccttg ttgcggaaga attgcagcag ctcggctatt cactggcgct
     9541 gggccgcgaa gtagttaatg aaagtagccg gatgggacta cctgatgaac tcactctaca
     9601 acgcgaattc gagcgcgctc gtcaacaggg tgcgctagca caatggattg cggtttttga
     9661 aggtggtttc actggtatcg tcgccaccct ggataccggt cgccccggtc cggtgatggc
     9721 tttccgtgtc gatatggacg cgctggatct cagtgaagag caggatgtca gccatcgccc
     9781 ctaccgcgac ggttttgcgt catgtaacgc cggaatgatg catgcctgtg gtcatgatgg
     9841 acataccgcc attgggcttg ggatggcgca tacccttaaa caattcgagt ccggactaca
     9901 tggcgtcatc aaactgattt ttcagcctgc agaggaaggt acgcgtggcg cgcgggcgat
     9961 ggtcgatgca ggtgtcgtag atgatgttga ttattttact gccgtgcaca ttggcactgg
    10021 cgtacctgcg ggcaccgtgg tgtgcggcag tgataatttt atggcaacca ccaaatttga
    10081 cgcgcacttc accgggaccg ccgctcacgc aggcgcaaaa ccagaagacg gtcacaatgc
    10141 cttgttggcg gcagcacaag caactcttgc actgcatgca atcgccccgc acagcgaaga
    10201 agcttccaga gtaaacgtgg gcgttatgca ggcaggaagc ggccgtaacg ttgttcctgc
    10261 ctcggcgttg ctgaaagtgg aaacacgcgg ggccagcgac gtcattaatc aatatgtttt
    10321 tgaccgtgca caacaagcga ttcagggcgc agcaaccatg tatggtgtcg gcgttgaaac
    10381 tcgtctgatg ggtgcagcta ccgccagttc tccttcgccg caatgggtcg catggttgca
    10441 aagtcaggcg gctcaggtcg cgggggtcaa tcaggccatt gaacgtgttg aagcgcctgc
    10501 gggttccgaa gatgccacat taatgatggc ccgcgtgcag caacatcaag ggcaagcctc
    10561 ctacgtggtg tttggcacac agctggcggc aggtcatcac aacgaaaaat tcgattttga
    10621 cgagcaggtt ctcgctattg ccgtcgaaac gctggcgcgc accgcgctca attttccctg
    10681 gacgcgaggt atctgatgca ggaaatctat cgttttatcg acgatgcgat tgaagccgat
    10741 cgccaacgtt ataccgatat tgccgatcaa atctgggatc atccagaaac acgttttgaa
    10801 gagttctggt cagcggagca tctggcttcg gcgctggaat ctgcaagctt caccgttacc
    10861 cgcaacgtag gcaatatccc aaatgcattt attgcttcgt ttggtcaagg caaaccggtt
    10921 atcgccctgc tgggagaata tgacgccctg gcaggtttaa gtcagcaagc tggttgcgcg
    10981 caacctacat ccgcgaggcc cggtgaaaat ggtcacggtt gcggacacaa tttgctggga
    11041 accgccgcct ttgccgctgc aatagccgtc aagaaatggc tggaacaata tgggcaaggc
    11101 ggcacagtgc gcttttatgg ttgtcctggc gaagaaggcg gctcgggtaa aacgttcatg
    11161 gtccgcgagg gggtatttga tgatgtggat gcggcactca cctggcaccc ggaagccttt
    11221 gccggcatgt tcaatacccg cacgctggca aacattcagg catcatggtg ctttaaaggg
    11281 atcgcagcac atgccgcgaa ttcccctcat ttgggacgca gcgcccttga tgccgtaacg
    11341 ttgatgacca ctggcaccaa cttcctcaac gaacatatta ttgaaaaagc gcgcgtacac
    11401 tatgccatca caaatagcgg cgggatctcg cccaacgtgg tccaggcgca ggcagaagtg
    11461 ctttatctta tccgtgcccc cgaaatgacc gacgtgcagc atatttatga tcgggtcgcc
    11521 aaaatcgccg aaggtgcggc attgatgacc gaaaccacgg ttgaatgccg cttcgacaaa
    11581 gcctgttcca gttatctccc gaatcgcacc ttagaaaatg ccatgtacca tgccctatcc
    11641 cattttggta ccccggaatg gaactccgaa gaactggctt ttgcgaaaca aattcaggct
    11701 acgctcaccc ccaacgatcg gcaaaacagt ctgaataata tcgccgcaac cggtggcgaa
    11761 aacggcaagg tttttgcact acgtcatcgt gaaacggtac tggcgaatga ggtcgctcca
    11821 tatgccgcca ccgataacgt gcttgcggca tcgactgatg tcggcgacgt cagttggaaa
    11881 ctgcctgttg cccagtgttt cagcccctgt tttgccgtcg gtacaccgct acatacgtgg
    11941 caactggtta gccaggggcg aacatctatt gctcataaag gaatgctgct ggcggcgaaa
    12001 actatggcag caaccacagt caatctcttc attgattcag ggctattgca agaatgccaa
    12061 caagagcatc agcaagttac ggacacgcaa ccgtatcact gccctatccc gaaaaacgtg
    12121 acaccgtcac ctttaaaata acaacaacgc aaacacaaca accgaggaat gcccatgagt
    12181 atgtcatcca taccgtcgtc ctcccaatcc gggaagctct atggctgggt cgaaagaatt
    12241 ggtaacaagg ttccccatcc ttttttgctc tttatctatt tgataatcgt actcatggtg
    12301 acgacggcaa ttttgtcagc ctttggcgtc agtgcgaaaa acccgaccga tggtacgccg
    12361 gtggtggtga aaaacctgct cagtgtggaa ggattacact ggtttttacc caatgtgatt
    12421 aaaaacttta gcggttttgc tccacttgga gcgatcctgg cgctggtttt aggtgccggc
    12481 ctagcggagc gcgtcggctt actgccagcg ctaatggtta aaatggcatc gcatgttaat
    12541 gcccgctacg ccagttatat ggtgctgttt attgcttttt tcagccacat ttcttccgat
    12601 gcggcgttag tgatcatgcc accgatgggt gcgctgattt ttctggcggt gggcaggcat
    12661 ccagttgcag gtttactggc tgccattgca ggcgtaggtt gcggctttac ggctaattta
    12721 ctgattgtca caaccgacgt gttgctgtcg gggatcagca cggaagcggc ggctgcgttc
    12781 aatccgcaaa tgcacgtcag tgtaattgat aactggtatt ttatggccag ctccgtagtc
    12841 gtactgacga ttgttggcgg cttgataacc gacaaaatca tcgagccacg gttaggtcaa
    12901 tggcagggaa acagcgatga gaaactgcag acattgaccg aaagtcagcg ttttggttta
    12961 cgcatagcag gtgtcgtatc gctacttttt attgctgcga tcgcgctgat ggtgatcccg
    13021 gaaaacggga tattgcgcga tccgattaat cacaccgtga tgccatcacc ctttattaaa
    13081 ggtatcgtgc cactaatcat tctttttttc tttgtggttt cgctggctta tggcatcgct
    13141 acccgcacaa ttcgacgtca ggcggattta ccgcatttaa tgattgaacc gatgaaagag
    13201 atggcgggat ttatcgtgat ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg
    13261 agcaacatgg ggaaattcat cgccgtgggg ctgaccgata tacttgaaag ttcagggctt
    13321 agcggcatcc cggcgtttgt cggtctggcg ttgctttcct ctttcttatg catgtttatt
    13381 gccagcggtt ccgcaatctg gtcgattctg gcccccattt tcgtaccaat gtttatgcta
    13441 cttggctttc acccggcatt tgcgcaaatc ctctttcgta ttgccgactc atccgtattg
    13501 cctttagcgc cggtatctcc ttttgttcca ctgtttcttg gattcctgca acgctacaaa
    13561 ccagacgcga aactgggtac ttactattcg ttagtcttgc cctatccgct tatctttttg
    13621 gtggtatggc tgctgatgtt gctggcgtgg tatcttgttg gtctgccgat aggtccgggg
    13681 atttacccac gtttgtctta agagagaacg gatgctgaga ttacttgaag aaaaaattgc
    13741 cacgccactg ggtccactgt gggtgatttg cgatgagcaa tttcgcctgc gggcggttga
    13801 atgggaagag tacagcgaac gcatggtgca gctgctggac atccattatc gcaaagaagg
    13861 ctatgagcgc atttctgcca ccaacccagg cggtttaagc gacaagcttc gtgattattt
    13921 tgccggtaat cttagcatta ttgatacgct tcccactgcc acagggggga cgccatttca
    13981 gcgcgaagtc tggaaaactc tacgcactat cccctgcggg caggtaatgc attacggcca
    14041 actggctgaa caattgggcc gccctggcgc ggcgcgtgcc gttggtgcgg caaacggatc
    14101 gaatcccatc agcatcgtcg taccttgcca tcgggttatt ggccgaaacg gcaccatgac
    14161 cggatatgca ggcggagttc agcgaaaaga gtggttattg cgccacgaag gttatctttt
    14221 gctgtaaaca ttaaacaatt tgtgccagct tgttcacact tttatgtaaa gttaccctta
    14281 acaacttaag ggttttcaaa tagatagaca tatatttaca tctaatatcg gaattctctg
    14341 ctgttaaggt ttgcttagac ttacttgctc cctaaaaaga tgttaaaatt gacaaatatc
    14401 aattacggct tgagcagacc tatgatcccg gaaaagcgaa ttatacggcg cattcagtct
    14461 ggcggttgtg ctatccattg ccaggattgc agcatcagcc agctttgcat cccgttcaca
    14521 ctcaacgaac atgagcttga tcagcttgat aatatcattg agcggaagaa gcctattcag
    14581 aaaggccaga cgctgtttaa ggctggtgat gaacttaaat cgctttatgc catccgctcc
    14641 ggtacgatta aaagttatac catcactgag caaggcgacg agcaaatcac tggtttccat
    14701 ttagcaggcg acctggtggg atttgacgcc atcggcagcg gccatcaccc gagcttcgcg
    14761 caggcgctgg aaacctcgat ggtatgtgaa atcccgttcg aaacgctgga cgatttgtct
    14821 ggtaaaatgc cgaatctgcg tcagcagatg atgcgtctga tgagcggtga aatcaaaggc
    14881 gatcaggaca tgatcctgct gttgtcgaag aaaaatgccg aggaacgtct ggctgcattc
    14941 atctacaacc tgtcccgtcg ttttgcccaa cgcggcttct cccctcgtga attccgcctg
    15001 acgatgactc gtggcgatat cggtaactat ctgggcctga cggtagaaac catcagccgt
    15061 ctgctgggtc gcttccagaa aagcggtatg ctggcagtca aaggtaaata catcaccatc
    15121 gaaaataacg atgcgctggc ccagcttgct ggtcatacgc gtaacgttgc ctgatttttc
    15181 cgcataactc actatccttc tgtcatatca ttaaattttt ctgatttatt gatctggcag
    15241 aaggttcatc actgtttcat tcaccagata tgggttaatc ttttaattac aaactgcgtt
    15301 gacagttgtt gtaaggagac cctgtatggc tatgtatcag aacatgctcg ttgttatcga
    15361 tcctaaccag gacgaccaac cagcattgcg gcgagctgtt tatttacatc aacggattgg
    15421 tggcaaaatt aaagcctttt tgccgatcta tgacttctca tacgaaatga ccaccctgct
    15481 ctccccggac gaacgtaccg ctatgcgtca gggcgtcatc agccagcgta cagcctggat
    15541 ccacgagcag gcaaaatatt atctcaatgc tggcgttccc attgaaatta aagtggtctg
    15601 gcataaccgt cccttcgaag ccatcattca ggaagtgatc agcggcgggc acgatttggt
    15661 gctaaaaatg gcgcaccaac atgaccgtct ggaagcggtg atttttacgc caacggactg
    15721 gcatctgtta cgcaaatgcc caagcccggt gtggatggtg aaagaccagc cgtggccgga
    15781 aggaggcaag gcgctggtgg cggtgaatct cgccagtgaa gagccgtatc ataatgcgct
    15841 caatgaaaaa ctggtcaaag agacgatcga actggcagaa caagtcaacc ataccgaagt
    15901 tcatctggtt ggcgcttatc cggtaacgcc aattaatatc gcgattgaac tgccggaatt
    15961 tgatccgagc gtttataacg atgccattcg tgggcaacat ttgctggcaa tgaaagctct
    16021 gcggcagaaa ttcggcatta atgaaaacat gacgcacgta gaaaaaggtc tgccagaaga
    16081 ggtgattcct gatttggcgg agcacttaca ggcggggatt gtggttctgg gcacggtagg
    16141 acgcaccggt atttcagcag cattcctcgg caacacggcg gaacaggtga ttgatcatct
    16201 tcgctgcgat ctgttggtca ttaaacctga ccagtatcag acacccgttg aactggatga
    16261 cgaagaagac gattaacgac tcttcaataa ctgacaaggc ccgagttatc gggcctattt
    16321 tttacttact gctcttcgcc gccaaggaaa taaattccca acgggattgc cagcaacaca
    16381 gtaaagacca ggctataaac aaaaatcatc gatgactgaa tcacatacat tgatgtcgtc
    16441 cactcagaca gcggaatatt gtactgttct ataacgccgc cgatagtcgc gcgagtcaca
    16501 accgacgccg caataatgaa aaccgccaac aaaccaaaga gaaatttctt ccctttcgct
    16561 gacttcagtt tcgccataat catgatgtaa tccttgtgca gacaaatttc ttgttaccta
    16621 aacgtaacaa taccatgaca tttcaccgtt gtctgctctg aaaacctcac ctcttagctg
    16681 gatttacaat caacgcattg attagttagc taatgttgct acttccttcc ctgactattg
    16741 atgatctgaa tgaattgtcg cggtaaaata gccccgccag gggtttccgg catgacgaca
    16801 attatctgga ggagcctgat aaaacaatga tcgctgaact gtttacaaat aatgcgctta
    16861 atctggtcat tattttcggt agctgcgcag cattgattct gatgagcttt tggtttcgcc
    16921 gcggaaatcg taaaagaaaa ggttttttat tccatgcggt gcaattttta atctacacca
    16981 taattatcag tgctgttggt agcattatta attatgttat agaaaactat aaactcaaat
    17041 ttatcacccc aggcgttatc gattttatct gtacgtccct gattgcggtt attctgacga
    17101 ttaagctatt tttgctgatt aatcagtttg aaaaacagca gattaaaaaa ggtcgcgata
    17161 tcaccagtgc gcggattatg tcgcgcatta tcaaaatcac cattattgtg gtgcttgttc
    17221 tgctttatgg cgaacatttc ggcatgagcc tttctggctt gctgaccttt ggtggtattg
    17281 gtggtctggc tgtcggtatg gccggtaaag atattctgag taatttcttt tccgggatca
    17341 tgctctattt cgaccgtcct ttcagtattg gcgactggat ccgttcaccg gacagaaata
    17401 tcgaaggtac agtagcggaa attggctggc gaattaccaa aattacgacc tttgataatc
    17461 gtccattgta cgtaccgaac tcgctgtttt cgtcgatcag cgtagaaaac ccaggacgaa
    17521 tgaccaaccg ccgcattacc acgaccattg gtttacgtta tgaggatgcg gcaaaagtgg
    17581 gcgttattgt cgaagccgta cgtgagatgc tgaaaaatca cccggccatc gaccagcgac
    17641 aaaccttact ggtttatttc aaccagtttg ccgactcgtc attgaatatt atggtttatt
    17701 gctttaccaa aaccacggta tgggctgaat ggcttgctgc acagcaagac gtttatttga
    17761 agattatcga tattgtgcag tcacacggcg cggactttgc cttccccagc cagacgctgt
    17821 atatggataa cattacaccg caggaacagg ggcgctaaat cagacatcac tggcgttatc
    17881 cacaagggta acgccagttc ccatcacatc aatgcttcac aatatacata gtccggctgt
    17941 acgccacatc ttccgggtta ttaatgggat aacctttcag ccacggcttg attaatcgtc
    18001 cattggtata ttgataaatt ggtgcaatcg gtgcttgctc catgaggatt ttttctgccg
    18061 cgttgtaatc ggcattacgc gctttaacgg tattttccgt cgatgcctgg gccagaactt
    18121 tgtcgtatgc cggattgtta aagcgtgaaa tatttcctga atgcgttgac gttaataatg
    18181 tcaggaaagt ggagggttca ttataatccc ccacccatga ggcgcggata acatcaaaat
    18241 tgccggtgtt acggctatcg atataggttt tccattcctg attttgcaat ttaacatcta
    18301 cgccaaggtt ctttttccac atcgatgcta cagcaatcgc aattttttga tggttttctg
    18361 aagtgttata caaaagcgtc agcttcagcg gtttttgcgg accataacca gctgcgctca
    18421 acaaagtttt tgcctgcgca ttcagttctt cctgactcat ttgttcaaac ggcgaaggtt
    18481 ccggcgtaaa tccggcggta acatctggtg taaaatgcca cgctggcttt tcgcccgtcc
    18541 ctaatacttt ttcggtcatc aggcggcgat ctatcgtcat acttaatgcc agacgaacgc
    18601 gctgatctgc cgtcggccct ttttgcgtgt taaacgcata ataataggtc ccgagctgcg
    18661 gcggcgtata aacctgcccc ggaatatcct tcaacagctt ctgatacata tttttcggga
    18721 aggattcggt gatatcaata tcccccgcga ggtaacgctt agtggctgcg gattcctgat
    18781 taattggcag gaaggtcact ttttgcagta ccgttttggc gttatcccaa taatgggtat
    18841 tcggtaccac gaccagtttt tcattgacta cgcgttcttt aagaacataa gcgccattgc
    18901 cgatcagatt tccgggtttc gtccactctt taccgctttc tacgttggct ttttgcaccg
    18961 ggaagaaggc aaagttagcg gttaaattca caaaccacgg caacggttta tcaagctgaa
    19021 ttttcaaggt atgggcatca actgcggtga cgccaagctg gtcaggcgta gctttaccat
    19081 caataattgc ctgtgcgttg ttgattcccg ccagcgcggc aaaccatgca aatggcgata
    19141 gcgttttcgg gtccaccaga cgttgccagc tgtagacaaa atcttgcgcc gttaccggtg
    19201 tgccatccgc ccattttgcg ttatcgcgca gggtaaaagt ccagatacgg ttgtcattac
    19261 ttttccactg agtcgcaacg ccggggacaa tctccccttt ctcgttctga ttcaccagtc
    19321 cttcaaacag atcgcgaatg acctgaatct ctggcaggcc cacggcttta gcgggatcca
    19381 gcgacgcagg ctcatcttta atgtggcgca ccagctcctg cttctctgcc agtactgtgc
    19441 cgctcggaac ttctgcagca tacgaaagag aaatgctgct gaccaacagc gcacaacacg
    19501 tgactgaaac agagtgcttc ataagtttcc ctctaaccga cattaaaatt gatgcgtaat
    19561 tatttgtttc gccttaaaga aatgcaaaca acttccatca aaaaagtgac acaggaagca
    19621 t
//
LOCUS       DAZFYC010000090        19564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFYC010000090 DAZFYC010000000
VERSION     DAZFYC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.090"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHKW5_003540"
     CDS             complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHKW5_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO7512873.1"
                     /translation="MINSINSFFSSIPRSISSVTRNSSFTASQHKSTPNTVKTSSPLS
                     PSNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
     gene            complement(727..1131)
                     /locus_tag="ACHKW5_003541"
                     /pseudo
     CDS             complement(727..1131)
                     /locus_tag="ACHKW5_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1214..1301)
                     /locus_tag="ACHKW5_003542"
     tRNA            complement(1214..1301)
                     /locus_tag="ACHKW5_003542"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Ser,seq:tga)
     gene            1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHKW5_003543"
     CDS             1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHKW5_003543"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO7512874.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGAC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHKW5_003544"
     CDS             2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHKW5_003544"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO7512875.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHKW5_003545"
     CDS             4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHKW5_003545"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO7512876.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLLLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHKW5_003546"
     CDS             5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHKW5_003546"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO7512877.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHKW5_003547"
     CDS             5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHKW5_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO7512878.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMISELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHKW5_003548"
     CDS             6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHKW5_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO7512879.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQV
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7329..8873
                     /gene="appC"
                     /locus_tag="ACHKW5_003549"
     CDS             7329..8873
                     /gene="appC"
                     /locus_tag="ACHKW5_003549"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO7512880.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8885..10021
                     /gene="appB"
                     /locus_tag="ACHKW5_003550"
     CDS             8885..10021
                     /gene="appB"
                     /locus_tag="ACHKW5_003550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7512881.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHKW5_003551"
     CDS             10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHKW5_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO7512882.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10202..11500
                     /gene="appA"
                     /locus_tag="ACHKW5_003552"
     CDS             10202..11500
                     /gene="appA"
                     /locus_tag="ACHKW5_003552"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO7512883.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSESELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSKLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHKW5_003553"
     CDS             complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHKW5_003553"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO7512884.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLDWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHKW5_003554"
                     /gene_synonym="yccY"
     CDS             complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHKW5_003554"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO7512885.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHKW5_003555"
     CDS             complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHKW5_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO7512886.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTANADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKIFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHKW5_003556"
     CDS             complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHKW5_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7512887.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHKW5_003557"
     CDS             complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHKW5_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7512888.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLHITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGTG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHKW5_003558"
     CDS             complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHKW5_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO7512889.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNIFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19029..19334)
                     /locus_tag="ACHKW5_003559"
     CDS             complement(19029..19334)
                     /locus_tag="ACHKW5_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512890.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT         4565 a         4972 c         5158 g         4869 t
ORIGIN      
        1 aggcgtttgg ccacatcagg tagaggccga tgtaaagcgt ttttttctat acttgactga
       61 gtataagatg tggaacgttg aagtcctgat tccgtataag aattttttac cttaaaaata
      121 gttgttgcac tggcaggact gttgctggga gaaagaggtg agctggtttt taccgtgtta
      181 ggtgtgcttt tgtgctgtga ggcagtgaag ctactgttac gcgtaacact tgatatactg
      241 cgaggaatgc tggaaaaaaa agaattaatg ctatttatca tatagataac cttataagaa
      301 ctttatgtga tagtctaatt tatccgtttg catgaaatct accttttgtg aaattctcgt
      361 caatatttta ctcaaaatga tcggctgtag gaataaacta tttgatatta ttgcgttttt
      421 agagttgttt tcaatcagct atagataggg cttggcgcca gaatggagaa tatttaacga
      481 gaaatagaag ctttgtatca gacgggcttc cctgaataga gaagttaaat aatcaggatg
      541 atgctaatga atatcaggaa caaggtgagt gtcttcatgt tcactgaaac agggtgtcac
      601 aaaaaatatg gctataagat gatcaatgtg atttgaaata tttataggtt tacagggggc
      661 tgggcatgca tatcttactg caatgcagaa tatgtgctgc ttggcgcagc tctttattga
      721 gaatgttcaa tcttttgttt cagtttcgtg aaggatacgg acggtttctt gtgtaatatt
      781 atacatcgcc tgtttatctt tactgcatac tcctgcaagt gttaatgcta ctccagcata
      841 aatttctgga taactatgtg taaatatatc cagtgagttt acatgtttct ctttcattcc
      901 tgcatcacaa cgaaccttat ttatacctgt cgccattgat tggggagggc tagagccttc
      961 tgcacgaaaa tagtgatgtg acagatctga gatagctttg tatccttctg cctggttcag
     1021 gcctggagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
     1081 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
     1141 ccaattggta taccaagttt ataccaagag ccaaaaaaca aaggggctac catttggtaa
     1201 ccccttgatt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     1261 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     1321 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     1381 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     1441 tttactcgtt tagcaactgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     1501 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aagtttcact
     1561 acgctttatt aacaatactt tctggcgacg tgcgccagtg cagaaggatg agctttcgtt
     1621 ttcagcatct cacgtgaagc gatggtttgc cttgctacag ggacgtcgct tgccgaccat
     1681 aagcgcccgg tgtcctgccg gtgtcgcaag gagaagagac gtgcgatatg aataacgagg
     1741 aaacatttta ccaggccatg cggcgtcagg gcgttacccg acgcagcttt ctcaaatatt
     1801 gcagtctggc tgccacgtcg ctgggattag gcgcgggaat ggcaccaaag attgcctggg
     1861 cgctggagaa caaaccgcgc attccggtgg tatggatcca cggtctggaa tgcacctgct
     1921 gtaccgaatc ttttatccgc tccgctcacc cactggcaaa ggacgtcatc ctttccctga
     1981 tttccctcga ttacgacgat actttgatgg ctgccgccgg aacccaggcg gaagaagtct
     2041 ttgaagacat catcgcgcaa tacaatggca aatatatcct cgcagtagaa ggtaatccgc
     2101 cgctgggcga gcaggggatg ttctgtatca gcagcggtcg accgtttatt gagaaactca
     2161 aacgtgccgc tgccggagcc agcgcgatta tcgcctgggg agcctgcgcg tcctggggct
     2221 gcgtgcaggc cgcgcgacct aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg
     2281 acaaacccat tatcaaagta cctggctgcc cgccgatccc ggatgtgatg agcgccatca
     2341 ttacttacat ggtgaccttt gatcgcttgc cagatgtcga cagaatgggc cgtccattga
     2401 tgttctatgg tcagcgaatc cacgataaat gctatcgccg cgcccacttc gacgccggag
     2461 agttcgtcca gagttgggat gatgacgctg cccgcaaagg ttactgtctg tacaaaatgg
     2521 gctgcaaagg gcctaccacc tataacgcct gttcctccac acgctggaat gatggcgttt
     2581 ctttcccaat ccagtctggt cacggctgcc tgggttgtgc ggaaaatggt ttctgggatc
     2641 gcggttcgtt ctacagccgc gtggtcgata ttccgcaaat gggtactcat tccaccgccg
     2701 ataccgtcgg tttaaccgcg cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg
     2761 ccagcgccgt tgaccagcgc agacgtcata accagcaacc tacagaaact gaacatcagc
     2821 caggcaatga ggataaacag gcatgagcac tcagtacgaa actcagggat acaccatcaa
     2881 taatgccgga cgccgcctgg tggtcgaccc gattacgcgc attgaaggcc acatgcgctg
     2941 cgaagtgaat attaacgatc agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt
     3001 tcgcgggctg gagatcatcc tgcaagggcg cgacccgcgc gatgcgtggg cgttcgttga
     3061 acgtatctgc ggcgtctgta ctggcgtaca cgccctggct tcggtttacg ccatcgaaga
     3121 tgccatcggt attaaagtgc cggacaacgc caatatcatc cgcaacatta tgctggcaac
     3181 gctctggtgc cacgatcatc tggtgcactt ctatcagctc gccgggatgg actggatcga
     3241 tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc gaactggcgc aaagtctctc
     3301 ctcatggccg aaatcatccc ctggctattt cttcgacgta caaaaccgcc tgaaaaaatt
     3361 tgttgaaggc gggcagttgg ggatcttccg caatggctac tgggggcacc cgcagtacaa
     3421 actgccgcca gaagccaacc tgatgggctt tgcccactat ctagaagctc tcgatttcca
     3481 gcgtgaaatt gtcaaaatcc acgcggtctt tggcggtaaa aacccgcatc caaactggat
     3541 tgtcggcggg atgccttgcg ccattaacat tgacgaaagc ggcgcggtcg gggcggtcaa
     3601 tatggaacgc ctgaacctgg tgcagtcgat tatcacccgc acggcagact tcattaacaa
     3661 cgtgatgatc cccgacgcct tagccatcgg tcagtttaac aaaccgtgga gcgaaatcgg
     3721 cacgggtctt tctgataaat gtgtcctcag ctacggcgca ttcccggata ttgccaacga
     3781 ctttggtgag aaaagtctgc tgatgcctgg cggcgcggtg attaacggcg acttcaacaa
     3841 tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag gagtttgtcg accacgcctg
     3901 gtatcgttat cccaacgatc aggtcgggcg tcatccattc gatggcatta ccgacccgtg
     3961 gtacaacccc ggcgatgtca aaggcagcga taccaacatt cagcagctga atgaacagga
     4021 acgctactcg tggatcaaag cgccgcgctg gcgcggtaac gcgatggaag tggggccgct
     4081 ggcacgcacc ttaatcgctt atcacaaagg cgatgctgcg accgttgagt cggtcgatcg
     4141 catgatgtcg gcgctgaacc tgccgctttc cggtatccag tcaacgttgg gccgcatttt
     4201 gtgccgcgcg cacgaagcac agtgggccgc aggtaagttg cagtatttct tcaacaagct
     4261 gatgaccaac ctgaaaaacg gcaatctcgc cactgcttcc acggaaaaat gggaacctgc
     4321 aacttggccg acagagtgcc gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg
     4381 ccactgggcc gccattcgcg atggcaagat tgatctctac cagtgcgtgg tgccgaccac
     4441 ctggaacgcc agcccgcgcg atcccaaagg gcagattggc gcttatgaag cagcgctgat
     4501 gaacaccaaa atggcgatcc ccgagcaacc gctggagatc ctgcgtactc tgcacagctt
     4561 tgatccgtgc ctcgcctgtt caacacacgt gctgggcgac gacggtagcg agctgatctc
     4621 cgtgcaggtg cgttaacagc gaaggagaat catcatgcaa cagaaaagcg acaacgttgt
     4681 cagccactat gtctttgaag cgccagtgcg tatctggcac tggctgacgg tgttatgcat
     4741 ggcggtgttg atggtcaccg gttactttat cggcaagccg ctaccttccg tcagcggcga
     4801 ggcgacgtat ctgttctata tgggctacat caggttaatt cacttcagcg ccgggatgat
     4861 ttttaccgtg gtcttgctga tgcggatcta ctgggctttt gttggcaatc gatactcccg
     4921 cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat
     4981 ccgctggtat ctgcttctgg caaaacgtcc gagtgccgat ataggccata accccatcgc
     5041 ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca ctggctttgc
     5101 gctgtacagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg tggtggaatt
     5161 tttctactgg acgggcggca actcaatgga cattcacagc tggcatcggc tggggatgtg
     5221 gctgattggc gcatttgtga tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc
     5281 cgacgacacg gtgatctcca ctatggtcaa cggctaccgt agccacaaat ttggcaaaat
     5341 aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg taatggggct gggcaacctg
     5401 ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac
     5461 tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt gctggggtat
     5521 gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct ggaacctgga
     5581 acgctgcgaa cctatgccgg agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc
     5641 ctgcaccaga acagtttctc cgaagtgctg gcgctggcag atatccgcgg acatctgcca
     5701 gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg acgactacgg cggcagcctg
     5761 agcgaactgg cgcgggagca actgcccgct gcggaacaat cggcgctggc gcaactggct
     5821 gcgtggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaatta tgactgtctg
     5881 tcgatggaaa attacgaagg cgttcgcttg cgccagtacc ggatgacaca ggaggagcag
     5941 ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga
     6001 cgccggtcag tgaatcccgt cttgacgact ggcttacgca agcgccagac ggcgtggtgt
     6061 tattaagcag tgacccgaaa cgtacgccag aggtcagcga taatccggta atgattagcg
     6121 aattactgcg cgagtttccc gactatacat ggcaggtggc gattgctgac cttgagcaga
     6181 gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc cgccacttta gtgtttaccg
     6241 gcggaaacta tcgcggtgtg ctgaatggta ttcacccgtg ggcggaactg ataaacctga
     6301 tgcgcgggct tgtcgaaccg cagcaggagc gtgcctcatg agcgaaactt ttttccatct
     6361 gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac tgccgatcac
     6421 ctgtcaggtg aatgatgaac cgagtatggc ggccctggag caatgtgctc acagcccgca
     6481 ggtgattgcg ctgttaaacg agttacaaca tcaactaagc gaacgccagc cgccgttggg
     6541 cgaggtgctg gcagtcgatc tgttaaatct caacgccgac gatcgtcact ttatcaatac
     6601 gcttctcggg gaaggggaag tgtcagtgcg cattcagcag gttgacgaca gtgaaagtga
     6661 aatacaggag gcgatcttct gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt
     6721 gctggaggac aaactggagg ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca
     6781 aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga tgaatggcct
     6841 accgttggcg catgagttac tggcacatgt acgtaacccc gacgcgcagc cgcacagcat
     6901 taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac gtctctgtgg
     6961 gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca acgccacggg
     7021 gttacgccat gtctggcatt tacgctgtac ggacacctta aaaggcccgt tactggaaag
     7081 ttatgaaatc tgcccaatac cggaagtggt gctggcagcg ccagaagatt tggtcgactc
     7141 tgcgcagcgg cttagcgagg tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga
     7201 cggtaagtat cgctttcagg cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga
     7261 gtttcaggat aaagagggag atctaccatt atcgggttat ttttctctct tcgcctacag
     7321 gagtgcgcat gtgggatgtc attgatttat cgcgctggca gtttgctctg accgcgctgt
     7381 atcacttttt atttgtaccc cttaccctgg ggctgatttt tttgctggct atcatggaaa
     7441 ccatttacgt ggtcaccggc aaaacgatct accgcgatat gacgcgcttc tggggtaagc
     7501 tcttcggtat caactttgct cttggcgtgg ctaccggcct gacgatggag tttcagtttg
     7561 gtactaactg gtcattctat tccaactatg tgggcgatat ttttggcgca ccactggcga
     7621 tggaagcatt aatggccttc ttcctcgaat ccacctttgt cgggctgttc ttcttcggct
     7681 ggcaacggct gaataaatac cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa
     7741 atctctctgc gttgtggata ttgaatgcca acggttggat gcaatacccg accggtgcgc
     7801 attttgatat cgacaccctg cgtatggaga tgaccagttt cagcgagttg gtctttaacc
     7861 cggtcagcca ggtgaaattt gtgcacaccg taatggcggg ctacgtgacc ggggccatgt
     7921 ttattatggc gatcagcgcc tggtatttac tgcgcggacg ggagcgcgat gtcgcattac
     7981 gctcgtttgc catcggttcc gtcttcggta ctctggcgat tatcggtacc ctgcaactcg
     8041 gagacagttc tgcgtatgaa gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag
     8101 gggagtggca aacggaacct gcacctgcac cgttccatgt ggttgcctgg ccggaacagg
     8161 atcaagagcg taacgccttt gctatcaaaa ttcccgcgct gctagggatc ctcgccactc
     8221 actcattaga taaacccgtg ccgggtctga agaatttgat ggctgaaacc tacccacgct
     8281 tgcaacgcgg acgtatggcc tggctgttaa tgcaggaaat atcgcaaggc aatcgtgagc
     8341 cgcatgtgtt gcaggcattc cgggagctgg aaggcgacct tggttacggc atgttgctct
     8401 cccgctatgc gccggatatg aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg
     8461 gcgcgatacc tcaggttgcg ccggtattct ggagtttccg catcatggtg ggctgtggtt
     8521 ccctgctgct actggtgatg ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc
     8581 agcatcgctg ggtgctgaaa atggcgctct ggagtttgcc gttgccgtgg attgcgattg
     8641 aagccgggtg gtttatgacc gagtttggtc gtcagccgtg ggcgatacag gacatcttac
     8701 cgacatactc cgcgcactcc gctttaacca caggacaact ggctttctca ctgatcatga
     8761 tcgtagggct ttacaccctg ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc
     8821 gtctggggcc gagcgcgatg cagagtgaac aaccgacgca gcaacagggg taaaggagaa
     8881 aatcatgttt gattatgaaa cattgcgctt catctggtgg ctgctgattg gcgtgatcct
     8941 ggtggtcttt atgatctccg acggatttga catggggatc ggctgtttgc tgccgctggt
     9001 ggcgcgtaat gatgatgaac gccggatagt gattaatagc gttggtgcgc actgggaagg
     9061 caatcaggtc tggttgatcc tcgcaggtgg agcattattt gccgcctggc ccagagtgta
     9121 tgcagcggcg ttttccggct tttatgtggc gatgatcctg gtgctgtgct cactgttctt
     9181 ccgcccgctg gcctttgatt atcgcggaaa aatcgccgat gcacgctggc gtaaaatgtg
     9241 ggatgccggt ctggtcatcg gcagtctggt gccgccggta gtcttcggta tcgccttcgg
     9301 caacttgctg ctcggcgtgc cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg
     9361 cagcttctgg caactgctga cgccattccc tttattgtgc ggattactca gccttgggat
     9421 ggtgattttg caaggtggcg tctggttaca actgaaaact gttggtgtga ttcatctgcg
     9481 ttcacagctg gcgactaaac gcgccgcact actggtgatg ctgtgctttt tgctggcggg
     9541 ttactggctg tgggtcggta ttgatggctt tgtactgctc gcccaggatg ctaacggtcc
     9601 ttccaatccg ttaatgaaac tggtagcagt gctacctggt gcctggatga ataatttcgt
     9661 cgaatcgccc gttttgtgga tcttcccgct gctggggttc ttctgcccat tgctgacggt
     9721 gatggcgatt tatcgtggtc gcccgggttg gggattttta atggcgtcat tgatgcaatt
     9781 tggcgtgatt ttcacggcag gcatcacgct gttccccttt gtcatgccgt caagcgtgag
     9841 tccaatctcc agcctgacgt tgtgggacag cacttccagt cagctgacgc tgagcattat
     9901 gttggtaatc gtgctgatat ttttgcccat tgtgttgctc tacactctct ggagctacta
     9961 caaaatgtgg ggacgcatga cgacagaaac tctccgccgt aacgaaaacg agctgtacta
    10021 aggagcaggg acaatgtggt atttactttg gttcgtcggc attttgttga tgtgttcgct
    10081 ctccaccctt gtgttggtat ggctggaccc gcgattgaaa agttaacgaa cgtaagcctg
    10141 atgcggcgca ttagcgtcgc atcaggcaat aatgtcggat atgaaaagcg gaaacatatc
    10201 gatgaaagcg atcttaatcc catttttatc tcttttgatt ccgttaaccc cgcaatctgc
    10261 attcgctcag agtgagtcgg agctgaagct ggaaagtgtg gtgattgtca gtcgtcatgg
    10321 tgtgcgtgcc ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc
    10381 aacctggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcttatct
    10441 cggacattac caacgccagc gtctggtggc tgacggattg ctggcgaaaa agggctgccc
    10501 gcagcctggt caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga
    10561 agccttcgcc gccgggctgg cacctgactg tgcaataacc gtacataccc aggcagatac
    10621 gtccagtccc gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataacgc
    10681 gaacgtgact gacgcgatcc tcagcagggc aggagggtca attgctgact ttaccgggca
    10741 tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat tttccgcaat caaaattgtg
    10801 ccttaaccgt gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact
    10861 caaggtgagc gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac
    10921 ggagatattt ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac
    10981 cgattcacac cagtggaaca ccttgctaag cctgcataac gcgcagtttt atttgctgca
    11041 acgcacacca gaggttgccc gcagtcgcgc caccccgttg ttggatttga tcatgacagc
    11101 gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct
    11161 gtttattgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg
    11221 gacgcttcca ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg
    11281 gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca
    11341 gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac
    11401 cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggccg gttttacgca
    11461 aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca
    11521 gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa
    11581 aaggcgttca cgccgcatcc ggtcacttta ccttcagttt tactctttct cggagtaact
    11641 ataaccgtaa tagttatagc cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt
    11701 gaggattgcg cctttaatat taacgccagc ctgttccaga cgctgcattg acaagctcac
    11761 ctctttggcg gtgttcaggc caaaacgcgc aaccagcagg ctggtgccga cagagcgtcc
    11821 cacgaccgca gcatcgctca ccgccagcat cggcggcgta tcgacaatca ccagatcgta
    11881 atggtcgttc gcccaatcca gtaattgacg catccgatcg cgcatcagca gttcagacgg
    11941 gttaggtggc acctgaccgc gagtaatcac atcaaagcct cctttgccaa aatgctggat
    12001 cactttgttg agctcatctt tacctgccag atattccgac aagccatgtt cattactcac
    12061 tgtaaacagg ttatgcgaat aaccgcggcg taagtcggca tcaataaata acactttttg
    12121 atcggactgg gcgatcaccg ctgccagagt tgaactgaca aacgttttac cactgtctgg
    12181 cgtcgcaccg gtgatcatca gaatgttatt ctccgtctcc atcatagcga agtgcagact
    12241 ggttcgtagc gcacgtacgg cttccacagc agaatccgcc gggttatcca cagccaggaa
    12301 ggggatattt ttagtacgat ggcgctgctg attagaaaat aaatttttct tacgcagacg
    12361 ggtacgttta tccagccact cggacattgg gatagtggca taaacgctga tgccgtgctc
    12421 ttccagttgt tccggggctt ctacaccacg acgcaacatc gcacgcgcca gcacggcacc
    12481 caccgaaata aacaggccaa gaataaaacc aagcaccaca ttcaacgctt ttttcggttt
    12541 cactggctgc ggctgagtga ctgccgggtc gataatccgc acgttaccaa tggcactgga
    12601 tttcgaaata ctcaactcct gctggcggtt aagtaattgc agatataccg cacggcccgc
    12661 ttctacgtca cgacttaaac gcaacacttc ctgttgggtg gaaggcatag ccgatacccg
    12721 cttattcagg cgtttgcgtt cttgctccag cgtctggcgt ttttccagca gcgcacgata
    12781 agttgggtga tctttcttat acagctggga gatctctgcc tcgcggaaag tcagctcatt
    12841 gagttgatta tcaacgttca caatctgctc aagaacggct ttggcttcca ggttaaggtc
    12901 aaccgaatcg cgctgctggc gataaacgtt gagtttttct tccgcctggt ccagctcgct
    12961 gcgcacttca ggcaactggc gctgtaagaa ttcaaggctt tgtgaatcct gcgccgcctg
    13021 gcgagcgata ttctgttgca aatagttgtt agcgatgctg ttcagaatac gagtaatcaa
    13081 ctggggatca tcaccggtca tggtaagttc cagcatcccg ctttctttac tgcgttcgct
    13141 aacggtaaag gtttcctgca acgcgttaat cgcttccagt tcggtacgtt ggctcaggac
    13201 aaactgtgtt cctggtttgg ccttaatgtc cgcgatagtc agcgcaacgc catctttttc
    13261 cagacgctgg ccgaccatac cattgacggt gaactcttca ccttccagcg tatagtggcc
    13321 gttttcccca accgtgagtg tcagttgctg atcctgacca ttcagttgtg gaatatgcat
    13381 ccagctgatc gccagctcac ctggtttctc tttggttaat ctcgcccagc cgcgacccac
    13441 aatcggaaaa tacttttgct caaccatgtc acgcagattc agttcagcaa tggttttacc
    13501 gagaatcatg cgcgattgca gcagttggat ctccggtgca gactcgggcg atgagttagg
    13561 gatcatatcg ctcaggccgc tgagaatggc gttgccctgt ttttgctcaa cctggaccag
    13621 agtatctgcc tgataaattg gtgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa
    13681 taacgcggtc acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag
    13741 cagatcgatc tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat
    13801 cgtattttat aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct
    13861 ccaacatacc gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct
    13921 ctttctgttc cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcgattg
    13981 cggtaacctg agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca
    14041 tctcagcagt gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa
    14101 catctgccgc tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga
    14161 cttttacgcc cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt
    14221 tgccggtaca aaccaccagg attgagttaa attttagttg ggccatgttt gaatccaccg
    14281 tgaagtctct gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg
    14341 gttccaacga gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa
    14401 ttccgttccg agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat
    14461 ttttccattc tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac
    14521 atcctgattc ataccctcag cgttacccag cgcttccgcc agggtcatac cgctgcggtc
    14581 cattttcagc gtgctctgtt tacccacttc acccatcacg aagattttca gatcatcgtt
    14641 gcgcggaata aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg
    14701 ctgcatcagc gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacatt
    14761 gcgccagtct gcatttgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag
    14821 aggaatattg gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt
    14881 ttgcgatcgg aacgctgcga cgctgacatc gacctgcggg ctttcaatga ctgaatcgag
    14941 acgcgctgta atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc
    15001 aatatagggg tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct
    15061 acggtattgc cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac
    15121 atcaccaatg ccgatacgat attcatagtt cgcgatttgc tgctccagct ccgggttagc
    15181 ctgcgacata atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat
    15241 attgaccatt ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact
    15301 ggtcgataaa ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt
    15361 cagtaccgaa aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat
    15421 gttatgaatt acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggata
    15481 gagctggtat ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac
    15541 caccgcgcgg ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa
    15601 acctttacta aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca
    15661 tacccctacc gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg cccctttatc
    15721 ttttgccaga tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt
    15781 ccagtaagcc gtcacgaaac ctgttggcgt ggaataatcg gtgaagcgca tcatgttgtc
    15841 ccagtcacgt tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg
    15901 gcgataaagc agctcggaac cgacaccggc gtacatcgtt tccagataac cgccatacac
    15961 ctggccatag aaaccattgc ctaagtcggc aaagtagttc gcctgcaagt tgttgagata
    16021 aacgtcattg cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc
    16081 gggtaatagc gaagacttaa acttgtcgta gttattgtaa atattggtga aaataccgcc
    16141 atcaagcagc aggtggtcag taaaccagta gcgggcgctg gacatcagcc ccaactggaa
    16201 catatagaaa tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct
    16261 aaagcgatct tcacgaatac ggtagccccg accaaaggca gaaagatctt cagcttcaac
    16321 acgttgttgt tgcagtggct ctgattgacc aggcgctgta ccggccagct gtttgcgcag
    16381 gctggctacg tcggtttcgg tagtcaccat agccatatgc tcgcgctttt gcgtcacgct
    16441 aatcttctca acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc
    16501 ttcacgagag tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag
    16561 ctgaatttct ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac
    16621 cgtggtgtac tgcaatcctt cagattcagg cgcaggttga tatgccggtt ttggcgtatc
    16681 gcgcagggca gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt
    16741 gttaccgcgt tcataactca ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc
    16801 gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc
    16861 gtcgtattcg agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa
    16921 gatggaagcc gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc
    16981 tgcgcgatga caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt
    17041 accggcgtag ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc
    17101 caccagatac tcaccgtcaa acagacccgt accagcaata tcacgtttac caaacgctac
    17161 ttgcggtagc cagtaacctt cttcccatag gcgaagttta aaatcgaaag atttgtcttt
    17221 gtatgactga tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt
    17281 ataacgaatg gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaacggta
    17341 ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg
    17401 catctgcaac aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta
    17461 ggttaatact tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata
    17521 agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt
    17581 aaatcagcat atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca
    17641 ggcggtggtt ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc
    17701 tcaggcgtaa tcaccataac gttattcttg tccgcgcctg caaggcgggg gtgatcttgc
    17761 agataatcgg taacgctacg gcctgcctgc cagggcaatt gcccagtccc agaaaccgca
    17821 cccagcagag taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccagc
    17881 gggggattgc tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga
    17941 ccagtgatat ggagattgag taactgctgg cgcacggatt taatcgttgc tgccacatcg
    18001 tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc
    18061 gctttagctt tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc
    18121 agttgcggtt gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt
    18181 tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg
    18241 taaagtacgc tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg
    18301 tcatttctac gggaataacc cctgcgccta acatttgtcg actttgacga acctggccag
    18361 tagccgaatc gacccagaat atgttttgcc actgtttttc tggcagcgtg gaagtcacct
    18421 cttcctgcca gacattacac agcacatcgc gacccgcaat attcagcgtc tctaatccag
    18481 caaaggaaaa agtggaactc agggtcgcag agcgaaaatg gttgtcttca ctccagagaa
    18541 tatcgcgcgt ccagcggctg ccgtctttta ttgccagcgc gttacgcaga gggtcctgcc
    18601 cggaatttgt cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat
    18661 tatgggtaac cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat
    18721 atcccagtac cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag
    18781 gcaacgcctg gatctgctga tccgctacgg tgatatcctg attatcgaaa aggctggcgc
    18841 gaaatgtatc gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa
    18901 tcgataaaat aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc
    18961 tctaaagccc ggtacgaata tcgcaaccgg gtattattta actttaattt cgacgcgttt
    19021 aagcagaatt agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca
    19081 ctgtcatttg ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca
    19141 actgtcgccg tgccgacagc gccagcagcc acgccaccag tggtagcagc cgtcgtggtt
    19201 gcaccggtag tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct
    19261 gcttcagggg cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa
    19321 agcttgtgtt tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc
    19381 aggattgtct actttgagta taacatcctt gtgaaatctc cctttcctca taccgcttgc
    19441 actatataca gcgggtgaga aaggcgcgca gagggaaata taagattgtt aacgatttcc
    19501 ctctggaaat acgacttgtg agcataagat aaatcttaga aaaatacaaa ttaaagataa
    19561 gaaa
//
LOCUS       DAZFYC010000091        19032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFYC010000091 DAZFYC010000000
VERSION     DAZFYC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.091"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            200..1030
                     /gene="yjiC"
                     /locus_tag="ACHKW5_003560"
     CDS             200..1030
                     /gene="yjiC"
                     /locus_tag="ACHKW5_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO7512891.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRKHERARMDTENIY
                     LPPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTETCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHTATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPSYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1171..1944)
                     /gene="uxuR"
                     /locus_tag="ACHKW5_003561"
     CDS             complement(1171..1944)
                     /gene="uxuR"
                     /locus_tag="ACHKW5_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO7512892.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2159..3619)
                     /gene="uxuB"
                     /locus_tag="ACHKW5_003562"
     CDS             complement(2159..3619)
                     /gene="uxuB"
                     /locus_tag="ACHKW5_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO7512893.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3700..4884)
                     /gene="uxuA"
                     /locus_tag="ACHKW5_003563"
     CDS             complement(3700..4884)
                     /gene="uxuA"
                     /locus_tag="ACHKW5_003563"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO7512894.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5224..6567
                     /gene="gntP"
                     /locus_tag="ACHKW5_003564"
     CDS             5224..6567
                     /gene="gntP"
                     /locus_tag="ACHKW5_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO7512895.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6741..6911)
                     /locus_tag="ACHKW5_003565"
                     /pseudo
     CDS             complement(6741..6911)
                     /locus_tag="ACHKW5_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6966..7663
                     /locus_tag="ACHKW5_003566"
     CDS             join(6966..7215,7215..7663)
                     /locus_tag="ACHKW5_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFO7512896.1"
                     /translation="MASVSISCPSCSATDGGVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(7938..8072)
                     /locus_tag="ACHKW5_003567"
                     /pseudo
     CDS             complement(7938..8072)
                     /locus_tag="ACHKW5_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(8042..8191)
                     /locus_tag="ACHKW5_003568"
                     /pseudo
     CDS             complement(8042..8191)
                     /locus_tag="ACHKW5_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8297..8473)
                     /locus_tag="ACHKW5_003569"
     CDS             complement(8297..8473)
                     /locus_tag="ACHKW5_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7512897.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTGSAALLRC
                     IEQAREGIRTLRQQ"
     gene            complement(8490..8981)
                     /locus_tag="ACHKW5_003570"
     CDS             complement(8490..8981)
                     /locus_tag="ACHKW5_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7512898.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGY
                     SLRVVDESDRTSADCTPPFVALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGTGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAGYLPGLPT
                     FDW"
     gene            complement(8978..9352)
                     /locus_tag="ACHKW5_003571"
     CDS             complement(8978..9352)
                     /locus_tag="ACHKW5_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7512899.1"
                     /translation="MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGASSQLINSIDILRARRATG
                     LMTRHNYRTVNNITLGKYPEAK"
     gene            complement(9442..9810)
                     /locus_tag="ACHKW5_003572"
     CDS             complement(9442..9810)
                     /locus_tag="ACHKW5_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7512900.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(9973..10194)
                     /locus_tag="ACHKW5_003573"
     CDS             complement(9973..10194)
                     /locus_tag="ACHKW5_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7512901.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERHKDSFGPYVRLMSVTLN"
     gene            complement(10257..10733)
                     /locus_tag="ACHKW5_003574"
     CDS             complement(10257..10733)
                     /locus_tag="ACHKW5_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7512902.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(10749..11234)
                     /locus_tag="ACHKW5_003575"
     CDS             complement(10749..11234)
                     /locus_tag="ACHKW5_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7512903.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(11289..12107)
                     /locus_tag="ACHKW5_003576"
     CDS             complement(11289..12107)
                     /locus_tag="ACHKW5_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7512904.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(12207..12440)
                     /locus_tag="ACHKW5_003577"
     CDS             complement(12207..12440)
                     /locus_tag="ACHKW5_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7512905.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(12519..12974)
                     /locus_tag="ACHKW5_003578"
     CDS             complement(12519..12974)
                     /locus_tag="ACHKW5_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO7512906.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(13050..15566)
                     /locus_tag="ACHKW5_003579"
     CDS             complement(13050..15566)
                     /locus_tag="ACHKW5_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512907.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            complement(15687..18806)
                     /gene="agn43"
                     /locus_tag="ACHKW5_003580"
                     /gene_synonym="flu"
     CDS             complement(15687..18806)
                     /gene="agn43"
                     /locus_tag="ACHKW5_003580"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO7512908.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASSD
                     NNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHGS
                     AQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGYN
                     GQATLNVTF"
BASE COUNT         4361 a         5056 c         4950 g         4665 t
ORIGIN      
        1 tgcttttaaa ttgcatgcaa ataattttaa atgagcgttt tttgatactt tagagtgttt
       61 cccaccattt aatatcactg tgtagagttg atatcactac aacagaaaca tgcatgtcat
      121 ttgtttactt ctctttttac tacagaaaaa tttatctatt tttctaagca tcagtgaaat
      181 actcataaag gtaactatta tgtctatgcc attaagcaat gcccttcaga gtcaaattat
      241 tacagataat cacttccttc atcatccaaa agtcgacagt gagttaacac gtaaacatga
      301 acgtgcacgc atggatacgg aaaatatcta tcttccgcct cttgctcgtg gcaataacca
      361 taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt gatattagta aagagtcctg
      421 gccatttaat tatgtgacag aaacatgcag agaattcaat ggtattacaa caacaggacg
      481 tatgctctat cgaaacttga aaatcacctc agctttagac gaaatatatg gtggtatatg
      541 taaaaaggcg catactgcca cagagttagc agaaggatta cggctaaatt tatttatgaa
      601 aagccctttc gatcctgttg aggactatac agttcatgaa ataacattag gccctggttg
      661 caatgttcct agttatgccg gaaccacgat aggatatatt tcaacccttc cagcttctca
      721 ggcaaaaaga tggaccaatg agcagccaag gattgatatc tatattgatc agataatgac
      781 ggtaacaggg gtagcaaatt cttccggatt cgctcttgcc gcattactta atgctaatat
      841 tgagttgggt aatgacccaa taattggtat agaagcctat cccggtaccg cggagatcca
      901 tgcaaaaatg ggatataaag ttatcccggg tgatgaaaat gcaccactca aacgtatgac
      961 tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa aatggtgaat ggaattatat
     1021 tggtaaataa ctaccaggaa gaatgagtac taacattcag gttatacaga tagctaaaca
     1081 atcccctgca atttacaaag taatactcat tgcgacttct taatttaaga aagcgcaagg
     1141 aacgtttacc cttgcgctta ttataataag tcaggcgtcg actttatcca gcggccatga
     1201 atcaaacaga tagccatcaa aataaatatc gtcaacgttc gagaattcca gcagacgttg
     1261 cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat cttttttgat
     1321 taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca ggctgtcatc
     1381 cagatggctg tgcaactgga tccacattgg attgttttcc cgccactgcc aggactgacg
     1441 gaacagctcc accagcatgc tgttatgcgt tgcttcggca atagcgagat ggaactgcat
     1501 gtcaccgctt tcgctgctgc ccggcgcact ggaagccagt tcacgctctt ccagttgcaa
     1561 tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agggcggcaa actcggcgat
     1621 gttgctctca agaagctgtc gcgcctgcaa cagctcaaaa ggacccgcat cattgcaaac
     1681 gttggcatcc ggactgtctg tgttgtggct gcctgagcta tcaagaacat agataccggc
     1741 accccggcgc acttccacca gccctttgat ctccagcatg atcagcgctt cacgtaccac
     1801 cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcaacc gttcgccagg
     1861 attgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct ggtaaggtct
     1921 ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta ggtgcggtta atcaaatttt
     1981 tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg gcaattttac ggcacccgtt
     2041 aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggca aaaaaacgaa
     2101 aactggtcaa ccaaatatca gtttgattga ccagtaagtc tgtagggtaa tcagttagtt
     2161 acagcgcagc cacacactcg cgcgcgccgc gttcgcacag ctgctgatat gccgccgtca
     2221 ccgcgccaac aaagtcggca ttctgcggca gatcatcggc aaaaataccg ctcaggccca
     2281 gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt aatcttctgg aactcggcca
     2341 gcatcgggtc gaccacgtca atggcattac cctgctcatc cacgccctgg gtgtaacgca
     2401 tccagccagc cacgcccagc gccaggtgac gccagctgcc gccgttttgc aggtgcagac
     2461 gcaccggatc cagcagacgc tgcggtaact tctggctgcc gtccatcgca atctgccagg
     2521 tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca taggcgttca
     2581 ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc agggcaaagg
     2641 ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat agtttcatag ccgccgaggt
     2701 aaccgagata tgccaggaaa gagtggctgc cgttcagcat acgcagcttc atcatttcga
     2761 acggcacaac gtctgcaacg aactgtgcgc ccactttatc ccagtccgga cgaccattaa
     2821 cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg cacgggtcat
     2881 aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc ggaacgatgc
     2941 ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat ccatgccgcc agctgcgggt
     3001 cacgcgcctg agccagcccc agtaccgcga ccttcgccac atgaccgttt tcacgcacgt
     3061 tatcgcagga catcaccgta aacgctttca gccctttttc acgacgcaga cgcagggctt
     3121 cgacgatgta accaatcgca gacttcggcg cagtcgggtt ttccagatcg tgcttgatca
     3181 gcgggttatt gagatccaac tgaccgcttg ccgcatcagc gcagtagcct ttttccgtga
     3241 ccgtcagaga gacaatcgcc gtctgcggac gcgccatcgc gttgagaata ccttcgcagc
     3301 cgtcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagt tcggtgcttt
     3361 ccgcgccttt ttccgccacg gtgtacagca gttgctgttt tttcaggttt tcaatcagca
     3421 cacggtcgtt acctggcatc aggttaactt cgcagatacc ccagtcgctg tcggtgcttt
     3481 ccagcagatg atgggtatac agcgcctggt gcgcgcggtg aaacgccccg caaccgagat
     3541 gcacaatgcg tgattccaga cgagaatgct cccatgacgg gcgggcaacc ggcagattgc
     3601 tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcagagagtg acggcaagga
     3661 agaaggaacc ggcacggcgc gcagccatgc cggtggatat taacggctaa agaaagcgcg
     3721 ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac caattgcgga
     3781 gtaacctggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt ggtccggacg
     3841 catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt caacaatcgc
     3901 tttcaccact tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt ggaaggtttt
     3961 cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg gaccgaactg
     4021 cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg tgcacatggt
     4081 gaaaccgttc gccatgctgt ttacggtatc aaccatccac tgcatatctt caatggtgga
     4141 aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa cagccatacg
     4201 gacgccaact tcttcggcaa ccggaataat cgctttcagg aagacggcaa agttttcgcg
     4261 cagtttggct ttgtcgatat ctttatatag ctccaggtgc ttacggaact ggtcgagggt
     4321 gtacccttct tccgcgcccg gaagaccagc aatgatgtta cgggtcagac gcgctttgtc
     4381 ttcgtcgctc atagtggcga agcgaacagc tgcctgagca atttcttctt cggtgtaatc
     4441 cgcttccgcg cctggacgtt tcaggatatg catttcgaat gcagcgaatt cgatctggtc
     4501 aaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac gggtccagtc
     4561 gagcaccggc atgaagttgt agcacacggt gcgaatgccg cactgcgcca ggttgcgcag
     4621 agtctgctga tagttagcga tccactgctc atagttgcca gtgtgggttt tgatatcttc
     4681 gtgaattggc acgctttcta cgacagacca taccaggcct gcgtcttcaa cgatcgcctt
     4741 gcgtttgagg atctcttcaa cggaccatac ttcgccgttc gggatatggt gcagcgcggt
     4801 aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat cgtttgggcc
     4861 gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt tccacagcaa
     4921 agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac cgcagccgtg
     4981 gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaatacat gtcaacccga
     5041 attctaaaat tggttaacca catcacaaga atttcactct cctggttgtt gctgcaaatt
     5101 gcagcaaagg gtaatcgatg gttattgatc agaggatgtg acattcatcg caacaatggt
     5161 tgaccaattt acataacatg tcgcccaaaa taacaacggt tcaaccaggt aaggtaaaac
     5221 gttatgcatg tgcttaacat tctctgggtg gtattcggca ttggtctgat gctggtactg
     5281 aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg tggcggcgct gtccgtcggg
     5341 atgctggcgg gcatggattt gatgtcgctg ctgcacacca tgaaagcggg cttcggcaac
     5401 acgctggggg aactggctat catcgtggtg ttcggtgcgg tcatcggtaa attgatggtc
     5461 gactccggcg cggctcacca gatagcacat acgctgctgg cgcgtctcgg tctgcgctat
     5521 gtacagctgt cggtgattat catcggcctg atttttggtc tggcgatgtt ctatgaagtg
     5581 gcctttatca tgttagcgcc gctggttatt gtcattgccg ccgaagctaa aattccgttc
     5641 ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact gttcccaccg
     5701 cagccgggtc ctgtggcgct ggtgaatgct tatggcgcag atatggggat ggtttatatc
     5761 tatggcgtac tggtgacgat cccaagtgta atctgcgcag gtctgatcct gccgaagttc
     5821 ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc ggtagatatg
     5881 aacaatctgc cctctttcgg cgtttcgatt ctggtgccgc tgatcccggc gatcattatg
     5941 atctccacca ccatcgccaa tatctggctg gtaaaagata cccctgcctg ggaagtggtt
     6001 aactttatcg gttcctcgcc gattgcaatg tttattgcga tggtggttgc attcgtactc
     6061 tttggcaccg cacgtggtca tgacatgcag tgggtgatga acgcttttga aagtgcggtg
     6121 aaaagcattg caatggtgat tctgatcatc ggtgcgggtg gcgtgttgaa gcaaactatc
     6181 atcgacaccg gcattggcga caccatcggc atgttgatgt cccacggcaa tatctcgccc
     6241 tacattatgg catggctgat cactgtgcta attcgtctgg cgacgggtca gggtgtcgtt
     6301 tcggcgatga ccgccgccgg gattatcagt gctgcaatcc ttgatccagc aaccggtcag
     6361 ctggttggcg tgaatccggc gctgctggtg ctggcgacgg ctgcgggttc caacaccctc
     6421 acccacatta atgatgcctc attctggctg ttcaaaggtt actttgacct gtcggtaaaa
     6481 gacacgttga aaacctgggg actgctggag ctggtcaact ccgtggttgg gctgattatt
     6541 gtgttgatta ttagcatggt agcgtaaaag aacaccagag cctgccaatg gcgagctctg
     6601 ttcggattgt cggcaaagtg cgctttgttt atgccagatg cgacgctgac gcgtcttatc
     6661 tggcctacaa agggctaacg tgcaggtttt tagcttcagg taatattgcg tacctgcatt
     6721 agcaatgccc tgtgatttct ttattgataa acaaaagtca cgccaataat cgattgcaca
     6781 ttccctgcag tcacctgccc tccggtacgt gcgtaatttg ccgttaatcc cagacttacc
     6841 gccgaagtcc ctactgctcc taacgatacc gtgttattcg ctggaataat cgtaccgttg
     6901 cgcatcaact gggtgatgct gccaacttac tgatttagtg tatgatggtg ttttgaggtg
     6961 ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac gggggggtgc
     7021 gtaacggcaa aagcactgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat
     7081 ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata
     7141 tggccatgaa tggcgttgga tgccgggcaa ctgcccgcat tatgggcgtt ggcctcaaca
     7201 cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg
     7261 cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc
     7321 gcgccagcgc tggctgtttt acgcgtatga caggcttcgg aagacggttg ttgcgcacgt
     7381 attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga
     7441 cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga agggaaagct
     7501 gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga atctgaggca
     7561 gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg agctgcatga
     7621 caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag tcattacccc
     7681 gaagttctta gattatcagc gttacatgca aacaaagtag tgaaaacaca ggtacaaaaa
     7741 aagccgccct tgagcgactc gatttgcata cgatgtggtg cgaagaccgg actcaaacat
     7801 gcgatttaac ctcatgattt aaaattgtta ataataaaca acaccatcct ataccaacac
     7861 agataccaac acgaaaaatc actggttttc ggcatcgaac caggacaatc aggtcctgat
     7921 tgagataccg agctctgtta ctcgtcatct tcctcgacag gctccattgc atcaagtggt
     7981 actcgcacca tcgcgataga accaatccgg ataaaagcat cttccccatc tatatcggtt
     8041 aactgatatc gcttacttga tgccggataa ttgtgtcgat tggcaacagt gtactctttc
     8101 gtacagccag aatgtggcag actgaggtgg aatagataac gtccgtatgc ccgctgacca
     8161 cctccgggcg ggagagtgtg gtatctgaca tcatcatttt tcctttctgt ttataaatga
     8221 aaacgccagc cgtgttcagg ctgacgtcag ggaagtgaaa tcgggtgagt gatcttcact
     8281 ggttctggtg caaaagttac tgttggcgca gggtacggat accctccctg gcctgttcga
     8341 tacagcgcaa cagtgctgcc gaacctgttt tatcctcatc gttgtcgaag ataattcccg
     8401 attcgcagtc gatattgtcc tgcagccacg taatcagaat atccagcgct gtttccgtgg
     8461 ttaatgattt catgttgtga atttccggat taccagtcga aagtgggtaa acccggcaga
     8521 tatccggcac tggcatccag atgaatgaga ctgacaccat aacgccggat gagtgtgacg
     8581 accagacgac ggaacgtttt tgacagcccc aggcgcttaa gccgcagaac cgggtatgac
     8641 cacgcatcgg tacgtaacag ataaccggta ccggtaaaat gaatccattc tgattcaccg
     8701 aagtcactgg tctggtgtga cagcgagtac agccaggcgt tgtccgtttc cgtgatatgt
     8761 gcggtactgc agcgtatgcc ggtaagggca acaaatggtg gcgtgcagtc tgctgaggtc
     8821 cggtcagatt catcaacaac acgaagggag tagccgttat cgcagaccat gttaatcagt
     8881 tcagcgagat taacaccatc aatgtcaacg acaattcgcc ccatattcag tgcctgtacg
     8941 ttaacactgt cggcttccag tgtcagggcc agtttcatca tttcgcctcc ggatacttac
     9001 ccagggtgat attgttcacc gttctgtagt tgtggcgggt catcaggcct gttgcccggc
     9061 gagcccggag aatatcaatg ctgttgatta actgcgacga ggctcctgcg ctgaatcccg
     9121 gctggtcagt tcgtaccagt gcgtattttt ccacgagaaa gttcactgca tcacacagtg
     9181 aaatgcctgc ctcaatatgc tgctcaatca cgcgttcatc cacgaacggt gtgtcattca
     9241 gcgtaaggct gtaatgctgg tccagtaacc gggtgagcag tgtctgccag atggtgacgg
     9301 gagacgggca gcacgatacc tcccgtacat gcgtgtcggg taatgttttc atactgaaga
     9361 ttttcctgat atgcagagat aaaaaatggg acagtggcgt ggtgaaaaca ccaggccgga
     9421 gcagaaggtt attctggtgg tttacttttt cgtttcaggt gtcggataaa cagccagata
     9481 aacgtaacca caggagccga gggtgtctgc ttcacaggtc agccccttcg catacagtgt
     9541 cacggtatgc tgatggcggg gattcagttc accactggtg agcattaatt tcagttgttt
     9601 catcagcagc ggaaaggcct ggtccagagg gtacgcatcc gcatctctga accggcctct
     9661 gataccggca cggtcggcaa ggtaatgcaa ccggttaccc tcctgcacca gacgggcgcc
     9721 gaaacagggc gtcactgtgc agggtagtcc ccaccaggga tggtcgtcat tgtcatcggg
     9781 atgcgttgtc ccggaaagtg tgtctgacac gataaaatcc tcacagaaaa tcggtgaatg
     9841 atagtttaac gatgacgggt cagccggtgc acagaccggc ttcggtgaag gtgtattcgt
     9901 tgatgagtag ggcctcatcc acagcggcgt ctgagctcag ccagtcgtac tcgttttccg
     9961 ggtgacggaa catcagttca gggtgacgct catcagccgg acatacgggc caaaactgtc
    10021 cttatggcgt tcagcaaaca cggccagcac accgggaata tcctgcactt cacgaccggt
    10081 atacgcttca gtgctgccgt gccagcggta tttgccggta cagaaaggaa aaagacggga
    10141 tgccggatgt tgttggtgaa tacgcatggc ttcaccacgg gtgatgattt tcatagtgtg
    10201 atacctctga agacagaaga taaaagtgaa aacaagcgtg atgtggttgt gacgggttaa
    10261 agcagaccat gttcggcaaa ggagaaaacc tggctgccac cgactatcag atggtccggc
    10321 acccggatat ccaccaggcc cagtgcctgt accagacgtt ccgtgataag gcggtctgcc
    10381 ttgctgggtg tgacttcacc ggacgggtga ttgtgtgcca gcaccacagc agcggcattg
    10441 tgatacaggg cgcgtttaat cacttcacgg ggatggactt ccgtgcggtt gatggtgccg
    10501 gtgaagaggg tttcaccggc aatcagctga ttctggttgt tcagatacag cacccggaac
    10561 tcttcacgct ccagtcccgc catgttcaga atcagccatt ctcgtgccgc acgggtggag
    10621 gtgaaggcca cgccgggttc atgaagatgg cggtccaggg ttttcagggc ccgttgaatg
    10681 aggctgcgct cgccgggcgt catctctccg ggcagaaagg aaagctgttg cattgtactt
    10741 ctctccattc agtcgatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa
    10801 tcccgcagcc ggtaataatg gaccgtcatg gcataacact ccgtacgaca ggcatgatga
    10861 ctgtacgtca tcagacaggc agcaatgccg gcagcttccg gactcatttc agcgcggtta
    10921 ccgttcatgg cattgaacag tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc
    10981 ataaatgccc cgccgttgtt cagggtgtac agattccaga taccaccgca gtagtcttca
    11041 cacagacggt ccatccagcc gaagacacgg ggctccaggg tcacccactg tggaatgagg
    11101 ccaaagtgct gcggccagaa gctgatgcgc tgttcatcag ggacgagggt ggcaaccaac
    11161 tgaggctggt cattccctga tgcagcggtt acggaaacag aagatgtggt ggaattatgt
    11221 gaaatggttt tcatgaaatg actccttaaa ggacaaataa atgcgtggtt ttcagggtaa
    11281 tggctgaatc agcgcaggct ctccagcagg gtttccgcca tcacccacaa tgcgcggttg
    11341 agcttaatgt cagtgtcaat gctgtgaatg gcacgggtat ggatacgttt tcctttagcg
    11401 ctgcgacctg aaattccgcc tttcagcata ttctcctgaa tggtctgata agcactccac
    11461 aggtccttac cgtaatcctc ccggcgtcgt ggcgtcagaa tgtcggcggt ggtgacgggc
    11521 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
    11581 ggcggcagaa tcagcgactg catggcatca cgcttttcct caatccggtc aaacacgccc
    11641 accacctcgt aagccccttc aatgactctg tccaccacat ttccccggtg cggaacacgc
    11701 acttccccca gagactgacc acagacgcat ccgttctggc agacgaacct gaagtaaccc
    11761 ggcagcatct ggtagctgga ggtaccgtca tgagagttga gcagaataat ttcagggaca
    11821 tgttctccgt ttatctctcc ggcccgccgc agacgcagca tatgttttgt gtatccccgg
    11881 cggcccgggt cgcgcacacg ggtctggcag gcgaagaatg gctgaaagcc ttcccgctgc
    11941 aggctttcca gtacggtgat ggtggggatg tacgtatagt tctggcttcg ggacgtgtgt
    12001 ttatcttccc cgaaaatccc cggcacatag tgcatcagtt cttcgtgtgt cagcggacgg
    12061 tcacggcgta tctggttcgc ataaccaaaa cgactggcta gtcgcataat ttgctcctta
    12121 tcggtggtta agatttactg gtgtaataaa tgaaaaagcc acgtctcccg gagaagacgc
    12181 ggcctgatgg gtgaaatgaa tgacgtttat tgtctgagaa gaccgtcact ggcgagccgc
    12241 gtattaagca cctttccggc atcgggttca gaattccggc agcgccagcg tcgctgacct
    12301 tccgcgttgc ggataacccg acggaaatgc attcccgggt catcctggat aaacacattg
    12361 cggtaagcgg ttatgacagc aaccgcctgc ccgtatgaga aagatccttc agccaggaca
    12421 tactctgtgt aacccggcat atctgttttc tcctgataaa taaaaagcct ctgccacgcg
    12481 ttgtgacaga ggctgaagca tgaaactgac ttcagggatc agttaacgtt ttttccggaa
    12541 acggtaatca gcagtggatg gtagtcctgg ggattgaaaa ccgataacgg cagactgaca
    12601 cgatggccgt tactttcttc agttgctttg atgatttcgg ttgtggcgac attttccacg
    12661 cactccgttt ccagaaatgc gtctgtggtt cgcgtggcat tactgtcacc aaaagcttcc
    12721 gtttccattt ttctggtcac cagcgtctga ccatatttgt ctttgagttg cagagtgatg
    12781 gtgagggggc caaatccttc atcgtttccg ccattatcca gccggaactg gtaagcacaa
    12841 atatttcccg ggagccatat cgtatctgta ttgcgtatac tgatgtaacg ttgatcctgt
    12901 gcccggagtg gggcagacca cgttaacccc agaatgaagg cggtaatcat gcaggttttg
    12961 aacaggtgaa tcatggtatt tacctctctg agtcatgacg atgacactga caaatcaggt
    13021 gataaagcgt aaaaggcgca gaatagccgt tatgccggta actccggggg taatgcttct
    13081 tccagtcggt taaccatatt gccgagatgg gatgcatcat attccatgac ggggcgttgc
    13141 ctgatgatac tgaccaccag tggtttgatt aacatattgg tcgcggcccg ttgttgtata
    13201 ccggcggcga aaatgatctc cagagcattc tgatcgataa cacgggccag attctcgttc
    13261 agattttcaa tctcatgtcc ggtcagcagg aaactgccgg caagccatgt cacctgctcc
    13321 tgaatcattg ccagaatttt gacgcgatct tcttcaacca gcgctccggc aatttttcct
    13381 gatacagcgg aggcaatcat tccccccaga acaccgccaa ccacacgccc tgtcagtgca
    13441 ccaaatggtc ccagtggact gaacagtatt ccgcccgcca ctgacccgac agcaccacct
    13501 gccacaccgg aagatgccac ggcaagattc ctgatgaact gcgtaccgga gatccgtccc
    13561 cgcaacattt tgatcatgtc agggccggtt gtgacagcta ccagtgccag agatgtcacc
    13621 agcgtaccct tcatcacttt gttcagggca ctgatatttc ctgcaccggt ccccttctga
    13681 agagccagtc gaacagtcgg gctggcagtc gaaaaatcaa tgtgcttcag cataccctga
    13741 acaacactga gccggtggag ttgctgggtt gtgacgtaga cagccagtgt gcgggtgaag
    13801 gtttttcctg cctggacagc agctgtctgc agggcagcat cgcgatcgcc ggtgctgagc
    13861 cagaagaccg atgccgtcag ggcaaaactg atccctccgg ccgccagact gacaaccgaa
    13921 ccctcggcaa tatcataagt gaccgattcg atggtcccga accgggtgat attacgggcc
    13981 tgagtataag tcagatgtcc ccgacgaatc agccgggatg cttctgcggg atcggttaca
    14041 ccgggtactt taccttcgcg gatcttattc ctcatggttt caacagcgcc ggcatactga
    14101 tcacggggga cttccagttg catggggcca ttatttccca tgtaacgata ctgtccgttt
    14161 tgtccgtcga atgccgcacc gacgctacgg gctgcagttg aacaatattt cgtctggatt
    14221 tctgttccgc tgaccagtct gtctgctcca tttctggcat tattatcacc gaggatccgt
    14281 gcgtttttca gacggactct gtcgacaata tggtttcccc gttcagcggc aaacccatgt
    14341 ccctgccctc cggaagcaaa cattttctca tttgtactcc aggtttccgg cagtttcatg
    14401 agaatcagac cattcgccgt tgcatcggaa ataaagctgt tgtaagggcg ttcataaatt
    14461 tcctgacaca gtgtttctgt ttcgcgacaa agagaggagc agtaccattc cggatccggc
    14521 ttatgatttt ccggggcatg atgtacttct gtcagcccgc aggaggcaca gatacgaagc
    14581 tcatccacgt gatggaatga tacaaaacgg cgaccttctg aaaagggata ccagtcaccg
    14641 ccgtccaggc aacgatcgag aaaatcgtca ttcgcttccg gtgataaatt caggcggttc
    14701 cggaagagcg ttttcagttc atcaggtgtg aatgacaggt tctctttcaa ctgttcgtta
    14761 aaccctgcca tgtttctttc ataccaggca ttgttcagta accagtttcc gtctgccagt
    14821 tcaaattccc tcagctctcc ccgggaaatc cgttgagaaa tgagcgtcag ccagatgcgc
    14881 tccgtcagtg agtcaggaag gctgtaatcg ttttgcaaac tggtcagcca cgatttactg
    14941 ttgagcaccg gttgttcttc cagtgtctgg ctgatggcac aggtcagttt tttcagcgcc
    15001 tggatttggc tggccgccca tagcagcgtc actaatgcag ccagccagta caacaacccg
    15061 ccccaccaca tcagtgacgg tgaaatatcg tactgtaacg cgatcaatgg tgcgcatgca
    15121 gaacacgcca taaggaaaaa ggcagtataa aaacagcagt aatacccgtt aatttccttg
    15181 ctgatatctt ctggtgtgat cccgttctgc tgaatgtttt tgatctgcag gggaacagcc
    15241 agcagaccac cggcgagagc ggtggtcaga ataaatttca cagagtcaaa ggtgtgagcc
    15301 tccagcgcga tatgtttacc atcaagtccc cacatgaagc tgatgatcat tccggcaatc
    15361 gtcagaaata cgcctgccag ccagcgaata tggaacaacc gggttatgcg ttcggtgata
    15421 tagccagcca gtaaggccgg gccgaacaga cacagcaata tgagccctgc cagcgcagaa
    15481 gccgtgctga ttaatgctct gaacaaaagg cgaagaatgg caaatcctgc gatcagcagg
    15541 atcagcgcgc cgactatctg taacatagtc actccgtgat gaatatcatg ttattgttaa
    15601 tgccagcgaa tgtggcgttg aatatccgtt gcgccggtcc gggtcccgat catgatgacc
    15661 gggaccacag agaggcgatg gttctgtcag aaggtcacat tcagcgtggc ctgaccgtta
    15721 tagccttcag cgctgctgcc gctgacgctg tggacataac ctgcctgaac gcccagggtg
    15781 atattttccc ggacacgggc ttccagtccg gcctgcaggt ccagtgacgt accattctgt
    15841 gacggtgaga acgtcatgtt actgccggct gcggctgtac ccatgctcat gtccccccgg
    15901 gagctgaagg tgcggataac agaaggctgt acccaccagt tcaccggcag ttcacgcaca
    15961 ctgtgtttgg cactgccgcg cagggtgtca cgggatgagg tgcctttacc aaagttcata
    16021 tcgtggtggc tgcccagacg gaagccggca cgcacatgtt gtgcactgcc atgcccgaac
    16081 ttcacataac tggcgttatc ctggccgtca tccagggaga gtccctgcca ggtgtactgc
    16141 agttgcggct ccagcatcag attgtcagtg atactgaagg gcagaccggt ttccagcgaa
    16201 cccagccagc cccagccccg gacgcggaag tcgttattgt ccgatgaggc tttcatgctg
    16261 tggcgggttc cctgggccac aatgtcagcc cacaggccgg aggcgttgtg tatcagattc
    16321 aggtatccgc ccaggctgcc ggcatcatcc cggaccgtgc cggcacggga gccgtcatca
    16381 tccttaacat caacggaaga atggccagca gcaccatata cccccgcggt cacagacata
    16441 ccggcaacct ctgttctgag caggtcaccc tccagacgga cgaagccata gctgccgctg
    16501 ctttccggcg tggctccacg ggcaataccg ccgttgttat cgtgaccgag atgaccgccc
    16561 tgaatgctga gacggacgct gttattttca ccgcttacac cggtctgatg gctgcgggag
    16621 cctgccagaa tccggtcata gtccattgcc tgtgtcagca tggaggcata cagggggact
    16681 tctgcacgat aagcattttc actgcgcaga taccagctct catcactgtc ccggttgagg
    16741 gagtagttaa aggcaccggc ctgcagctta ttcccctgga taaaggcccc ttcctccgtg
    16801 gtggcaccgt taatggcttc caccacctga atacccttac cgctggtcgc cagccccgac
    16861 gcactgttgc cggcgttcac caggttcagg atggtttttc cggttgccct gccaccgtca
    16921 atgaccagtc tgtcagcatt gttctgtgcc atatccgggc gtacacgcag gctgatggtg
    16981 ccattctgtc cgttcaggtt tttcactttc agggttgccg gtacgaactt ccctgtgcgg
    17041 gtggaggtga aatgaatctg tccggcatgg ctgaggtcat ccaccaccga ctgcaccgtg
    17101 gcgttatcgg gaatattcca ggtggcacca gaggcgagag tgacattcgt ggggtcaatg
    17161 gcaccgttca gcacagtgct gccggtcagc ttcagggctg taccgcgctg agcaatgaca
    17221 tccgtggtga cggtactgtc gttcagcgtc agcgtgcctt cattcaggtt gacggctttc
    17281 tgggtgagtg tggtgttgct gacagtgagt gtgccacttc ctgatttttc cacgctgccg
    17341 ttaccggtga gagagcctcc ctgcaggagt gcatcgcctt cacggatggt cagggtatcg
    17401 ttgttcaccg tcttcccgga aagggtaagt atggcgccgt tattcagcgt ggtggtgccc
    17461 gccagtgtgc cgcccctggc ggtgaacagt ccgccattta ccgtggtatc cgtggccgta
    17521 tcaccggcgt tcagcgtgaa tgaactgcct ttttccagca tcagggcatc cgcctgaccg
    17581 cctccgatac tgaatgcctt tccgtcgctc cgggtaccac tgacagcggc accggaatcg
    17641 gccagcagta caccgccatt tcccatggat acggtggtgg cggtgccacc gttgcggaca
    17701 tccagcgttc cgccatcatc cacgcgggta ttagtggctg tgtgtccggt cagcacatcc
    17761 aggcgtccgc cattttccag tacgacatta tcagctttac cctccacaac agagaatgct
    17821 cccaggcggt ttatgccggt aacggttgca gccgtactgg taaccagtgc gccgccctgc
    17881 ttcagggtga cattcgtggc tgtaccaccg gcgtccacct gcagtctgcc cttctgatta
    17941 acggtggtat tcccggccac ccccccgttt ttgacaatct gccagccgtc actgtttaca
    18001 acagtgccag acgccgtacc gccgttgtgc acatactggt accccccatt cagcgtggta
    18061 tccagtgcgt gaccatgtac agtctggtcg ccgccggcat aaaccacagt ggtatttgcc
    18121 gttccttcag cagccacaat ctgacgacca tttttattga tggtggtgcg tacggcatct
    18181 ccgtagacgg tctgcccggt atcaccattt tccgcatccg gtcccccttc cgcgccggta
    18241 ttcacaaccg tgtcggttgc cactgcgccg gatttgatgg cctgccagcc cttctcatta
    18301 atgacggtac ccgttgcaat cccgccttca tgtacccact gctcaccgcc gttcagagtg
    18361 gtgttcactg cctgcccctg aaggctctgt ccgcctccgg cactgataac cgtgtctgaa
    18421 acgcttcctc cggcattcac tctctgaaga ccaccaccgg tgacagtagt gttgttggcg
    18481 ataccgccat tttgtatcca ttgtccgccg gtattggcct cgttatccgg cccatactcc
    18541 agaccggtac tgatggtcat tccgttggcc gtaccgagga caatctggtt gtcatgattt
    18601 gtcagtgttc cgtcgttcac ggtttctccc gcctgtacaa ccttgtcagc agccagtgcc
    18661 gggactgatg tgacagcagc aagagacagc gcaaccgcca caccagcgcg ttttccccgt
    18721 gagcgggcca gttcggaggc caccaccagg gtgcccgtaa tgtgattcca taccagcctg
    18781 tagctggtgt tcagatgtcg tttcatcagc ttttccttac agagggtgaa taaaaaagcc
    18841 ggtacccaca aatgtggata ccggcaggta caaaacagca tgctcagata aagtggtttt
    18901 tcccggacat aaatgcttcc cctctctcat ccgccagaga agtatcagtc cgttgtgtgc
    18961 aattccgttc tgcatgatga agagattcag ctgccttttc gcagaccatt aaccactata
    19021 aacgatcgat ag
//
LOCUS       DAZFYC010000092        18590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFYC010000092 DAZFYC010000000
VERSION     DAZFYC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.092"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..886
                     /locus_tag="ACHKW5_003581"
     CDS             <1..886
                     /locus_tag="ACHKW5_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFO7512909.1"
                     /translation="RTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKAS
                     DKDKERVTKNINIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTD
                     RSIIRAMMEGGTAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVS
                     DTPLDDKEKGFISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQ
                     ARAMVATGNYDEAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLS
                     ARMLSRYQNNYHFGGSTL"
     gene            883..3704
                     /gene="traG"
                     /locus_tag="ACHKW5_003582"
                     /pseudo
     CDS             883..3704
                     /gene="traG"
                     /locus_tag="ACHKW5_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490585.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
     gene            3719..4222
                     /locus_tag="ACHKW5_003583"
     CDS             3719..4222
                     /locus_tag="ACHKW5_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO7512910.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            4254..4985
                     /gene="traT"
                     /locus_tag="ACHKW5_003584"
     CDS             4254..4985
                     /gene="traT"
                     /locus_tag="ACHKW5_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO7512911.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            5237..7390
                     /gene="traD"
                     /locus_tag="ACHKW5_003585"
     CDS             5237..7390
                     /gene="traD"
                     /locus_tag="ACHKW5_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7512912.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDLSGEFVKSYYDPSIDKILNPLDAHCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            7390..12660
                     /gene="traI"
                     /locus_tag="ACHKW5_003586"
     CDS             7390..12660
                     /gene="traI"
                     /locus_tag="ACHKW5_003586"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO7512913.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMTE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLNELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGNISGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFADLGREINTQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPGSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREV
                     VRENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            12680..13426
                     /gene="traX"
                     /locus_tag="ACHKW5_003587"
     CDS             12680..13426
                     /gene="traX"
                     /locus_tag="ACHKW5_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO7512914.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSR
                     VGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            13485..14345
                     /locus_tag="ACHKW5_003588"
     CDS             13485..14345
                     /locus_tag="ACHKW5_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7512915.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            14448..15008
                     /locus_tag="ACHKW5_003589"
     CDS             14448..15008
                     /locus_tag="ACHKW5_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO7512916.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDETVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            15141..15353
                     /locus_tag="ACHKW5_003590"
     CDS             15141..15353
                     /locus_tag="ACHKW5_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7512917.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            15598..16059
                     /locus_tag="ACHKW5_003591"
     CDS             15598..16059
                     /locus_tag="ACHKW5_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO7512918.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            16105..16314
                     /gene="hha"
                     /locus_tag="ACHKW5_003592"
     CDS             16105..16314
                     /gene="hha"
                     /locus_tag="ACHKW5_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO7512919.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            16352..16504
                     /locus_tag="ACHKW5_003593"
                     /pseudo
     CDS             16352..16504
                     /locus_tag="ACHKW5_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16505..16911)
                     /locus_tag="ACHKW5_003594"
                     /pseudo
     CDS             complement(16505..16911)
                     /locus_tag="ACHKW5_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17033..17470)
                     /locus_tag="ACHKW5_003595"
     CDS             complement(17033..17470)
                     /locus_tag="ACHKW5_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512920.1"
                     /translation="MYFIGYHGTSEASAQNILSVGVQERFLPPTGQIGRGFYIAKMNG
                     TLPQWGAENATEFARRNQPYFTRMVSLLTGASNNPWLDKAAQRTILKIYTVRPLLHCK
                     WNIMNPGDLEFLKATLYDNGGPGLDLKRDCKWLYHQRNSSTCG"
     gene            complement(17719..17898)
                     /locus_tag="ACHKW5_003596"
     CDS             complement(17719..17898)
                     /locus_tag="ACHKW5_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512921.1"
                     /translation="MGNILFDEKNNRFWPIDFSNSYEHYYSIDKDSKLFVNKMADREF
                     CSILKTLRENAISNI"
     gene            18120..18473
                     /locus_tag="ACHKW5_003597"
     CDS             18120..18473
                     /locus_tag="ACHKW5_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512922.1"
                     /translation="MRQVLNINVGLGSDIPAGHSPSIFEACLHAITASKALNDGGNSQ
                     LSSEVWGYSGASVSFREAFWLATGGDGKILDLPIGKLRKDYFVDTIVIDTNGHNLDII
                     IYDDTTEDILQKLII"
BASE COUNT         4665 a         4255 c         5364 g         4306 t
ORIGIN      
        1 ccggacgcag gtgtcccagc agaaagtctg tcaggacatt gccggtgaga gcaatatttt
       61 tgctgactgg gcggcttccc ggcagggatg taccgttggc gggaaatctg acagtgtcag
      121 ggataaagcc agcgacaagg ataaggagcg ggtgaccaaa aacatcaaca tcatgtggaa
      181 tgcgctttcc aaaaacagaa tgtttgacgg caacaaagag ctgaaagagt ttgtgatgac
      241 gctgaccggc tcactggtgt ttggtcctaa cggcgaaatc acacccttgt cagccagaac
      301 cactgaccgc tcaattatcc gggccatgat ggaaggcggc acagcaaaaa tttatcactg
      361 caacgattct gataaatgcc tgaaagtggt ggcagacaca ccggtgacca tcagccggga
      421 taatgcactg aagtctcaga ttactaaact tctggccagc attcagaaca aggcggtcag
      481 tgacacgcct ctggatgaca aggaaaaagg ctttatttcc agtaccacca tccccgtctt
      541 caaatacctg gttgacccgc agatgctcgg tgtttccaac agtatgattt accagctgac
      601 ggactatatc ggttacgaca tcctgctgca gtacattcag gagctgatac agcaggcccg
      661 ggcgatggtg gccacaggaa attatgacga agcagttatc gaacatatta acgacaacat
      721 gaatgatgcc acccggcaga ttgcggcgtt tcagtcacag gtgcaggtac agcaggatgc
      781 gctgctggtt gtcgatcgtc agatgagcta catgcgtcag cagctttccg cccgcatgct
      841 cagtcgttac cagaacaact atcacttcgg agggagcacg ctgtgaatga agtttatgtg
      901 attgccggtg gtgagtggtt gcggaataac ctgaacgcca ttgccgcctt tatgggaacc
      961 aggacgtggg attccattga aaaaattgcg ctcacattgt ctgttctcgc ggtggccgtg
     1021 atgtgggtac agcggcacaa cgtgatggat ttgctgggct gggtggccgt gtttgtgctt
     1081 atcagcctgc tggttaatgt ccgcacatcg gtgcagatta ttgataacag tgacctggtc
     1141 aaagttcacc gggtggataa tgtaccggtc ggactggcga tgccactttc actgacgacc
     1201 cgtatcgggc atgcaatggt ggccagttac gagatgatct tcacgcaacc ggacagtgtc
     1261 acctatagca aaacggggat gctgtttggc gcagaactgg tatcaaaaag caccgacttt
     1321 ttgtcccgca atccggaaat cgccaatctt ttccaggatt atgtgcagaa ctgcgtgatg
     1381 ggggatattt acctgaacac aaatacacgc tggaggagct tatggcctcc gctgatccct
     1441 acacgctgat tttttcccgc cccagtccgc tacggggcgt ttatgacagc aataataatt
     1501 tcgtgacctg taaggacgcg tcggtttcgc tgaaagacaa gctgaatctg gatacacaga
     1561 gtggcggaaa aacctggcat tattatgccc agcagctatt tggtggacgc cctgatccga
     1621 acctgttgtt cagtacgctg attggcgaca gttacagtta cttctatggc tcaagtaaat
     1681 cagccagcca gatcatccgc cagaatgtca ccatcaatgc gctgaaggag gggatcacca
     1741 gttatgccgc gcgtaatggt gattcagcca gcctggtgaa tctggccacc acgtcatcga
     1801 tggagaagca acgtctggca catgtctcca tcggtcatgt ggcgatgcgg acactgccca
     1861 tgacgcagac catcctgacg gggatcgcca tcggtatttt cccgcttctg gtactggcgg
     1921 ctgtcttcaa caaactgacg ctgtccgtcc tgaaaggcta tgtgtttgcc ctgatgtggt
     1981 tgcagtcatg gccaatgttg tacgccattc tgaacagcgc catgacattt tatgcgaagc
     2041 agaatggtgc accggtggtg ctctcggaaa tttcacagat acagctgaaa tactccgatc
     2101 tggcttccac tgccgggtat ctttccatga tgatcccgcc attatcctgg atgatggtca
     2161 ggggactcgg ggccggattt tccagtgtgt acagccattt cgcttcctct gctatcagcc
     2221 cgactgccag tgcggcaggt agtgtggttg acggtaatta ctcctacggc aacatgcaga
     2281 cggaaaacgt gaacggattc agctggagca ccaacagcac cacgtcgttt ggtcagatga
     2341 tgtaccagac cggcagtggc gcaaccgcta cacagacccg tgacggtaat atggtgatgg
     2401 atgcgagcgg ggcgatgtcc cgtttaccgg tcggtatcaa tgcaacgcgt cagattgcgg
     2461 cggcacaaca ggaaatggcc cgggaggcgt cgaacagagc agaaagcgcc ctgcatgggt
     2521 tcagcagcag tattgccagt gcctggaact cgctcagtca gtttggttct aaccggggga
     2581 gcagtgattc tgtcaccggt ggtgctgaca gcacgatgag cgcacaggac tccatgatgg
     2641 ccagccgtat gcgcagcgca gtggaaagct atgcgaaggc gcataatatc agtaatgagc
     2701 aggcgacaag agaactggca tcaagaagta cgagaggttc tgcggggatg tatggcgatg
     2761 ctcatgctga atggggggtt aaacctaaaa ttcttggtgt tggtggtggc gtaggtgtta
     2821 gaggtggtgg tcgggcaagt attgactgga gtgatgagga tgcgcatcag gccagcagtg
     2881 gttcacgggc cagccatgat gctcgccatg atatcgatgc cagggctaca caagacttta
     2941 aagaggccag cgattacttt accagtcgca aggtcagtga atccggcagc catacggaca
     3001 ataatgccga ttcccgtgtg gaccagctgt ctgcagccct gaactcagca aaacagagtt
     3061 acgaccagta cacgacgaat atgacccgca gccatgaata tgctgaaatg gcatcccgta
     3121 ctgaaagcat gagtgggcag atgagtgaag acctgtcgca acagtttgca cagtatgtga
     3181 tgaaacacgc gccgcaggat gccgaagcta ttctgacaaa caccagttca ccggagattg
     3241 cagaacgccg tcgggctatg gcgtggtctt ttgttcagga acaggtgcag cctggtgttg
     3301 ataatgcctg gcgtgaatcc cgtggggaca tcggtaaagg aatggagagc gtaccttcgg
     3361 ggggtggcag ccaggatatt attgctgatc atcaggggca tcaggccatt attgagcaaa
     3421 gaacgcagga cagtaatatc cgtaatgatg taaaacatca ggttgataat atggtcacag
     3481 aataccgagg caatatcgga gatactcaga gcagcatacg tggcgaagaa aatattgtag
     3541 gaagacagta ttctgaacta caaaatcacc atataacata ggcattatct cagaataata
     3601 aatataatga agaaaaatcg ggtcaggaaa gaatgcccgg ggctgacagt ccacaggaac
     3661 tgatgaaaag agcaaaggag tatcaggata agcacaagca ataatattgt atggggagat
     3721 gataatgaaa aatttagctc atattgctct ggtgacggtg atacagttca ttgcctgtta
     3781 tctggctgat tgggggagtg ccgaaacagt ttttatcctg tgctttattg tactatggca
     3841 gggattgttt atatggttat tttctcaaat acgaaaaaaa cggcatgtgt cggatgagtt
     3901 taaattcagt aaaggcgttt ggtatatact gatgccagta tcttccttac tctctccgtt
     3961 attaagcctg atggttttta tcattggtac actatacgaa ttaaggcgaa ttagtggttg
     4021 tgtaagtgta agagaatgga tgcaatctca ggtaaatgaa caaactaatg aagatttgca
     4081 tctggatttt gatgatgtga attttgatta tgtggatttt tatcgtacta atccggcaac
     4141 aggattagct atgcatggag gatttgattc agcaggaaat acttttggga cttgttggca
     4201 ggactatgat gacaggcgat aagaaaactg tggtttgcaa taaaggtaat ttgatgaaaa
     4261 caaaaaaatt gatgatggtt gcactggtca gttccactct ggccctttca gggtgtggtg
     4321 cgatgagcac agcaatcaag aagcgtaacc ttgaggtgaa gactcagatg agtgagacca
     4381 tctggcttga acccgccagc gaacgcacgg tatttctgca gatcaaaaac acgtctgata
     4441 aagacatgag tgggctgcag ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg
     4501 tggtgacttc tccggataaa gcctactact ggattcaggc gaatgtgctg aaggccgata
     4561 agatggatct gcgggagtct cagggatggc tgaaccgtgg ttatgagggc gcagcagttg
     4621 gtgcagcgtt aggtgccggt atcaccggat ataattccag ttctgccggt gccacactcg
     4681 gtgtgggcct tgctgccggt ctggtgggta tggctgcaga tgcgatggtg gaagatgtga
     4741 actataccat gatcacggat gtgcagattg cagagcgtac taaggcaacg gtgacaacgg
     4801 ataatgttgc cgccctgcgt cagggcacat caggtgcgaa aattcagacc agtactgaaa
     4861 caggtaacca gcataaatac cagacccgtg tggtctcaaa tgcgaacaag gttaacctga
     4921 aatttgaaga ggcgaagcct gttctcgaag accagctggc caaatcaatc gcaaatattc
     4981 tctgattatc ttccggaggc tggtctgacc ggcctcctga ctgacgcctc gctttgctcg
     5041 ttgtccaacc ccggaaagaa ataaaataaa ttttatttct ccccggcgtt tccgaaataa
     5101 atatgaaaac actgagcggt tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga
     5161 agccggaatt taacggttat taatcttttc atcattaata atcagtccga ctgacttttt
     5221 tcttcggaat atcatcatga gttttaacgc aaaggatatg acccagggcg gtcagattgc
     5281 gtccatgcgt atccgcatgt tcagccagat cgccaatatc atgctttact gcctgtttat
     5341 ttttttctgg atactcgttg gtctggtttt atgggtaaaa ataagctggc agacgtttgt
     5401 gaacggctgt atttactggt ggtgtaccac gctggaaggc atgcgggatt taatcaagtc
     5461 ccagccggta tatgagatcc agtattacgg caaaaccttc cggatgaacg ctgctcaggt
     5521 actgcatgat aaatatatga tctggtgcgg agagcagcta tggtccgcat tcgttctggc
     5581 tacagttgtg gcactggtta tttgcctgat taccttcttt gttgtctcct ggattctggg
     5641 gcgtcagggt aaacaacaga gcgaaaatga agtcacaggt ggtcgtcagc tgacggagaa
     5701 cccgaaagac gttgcccgga tgctgaaaaa agacggcaag gactccgata tccggattgg
     5761 cgacctgccg attatccggg attccgaaat ccagaacttc tgtctgcacg gcacggtcgg
     5821 ggccggtaag tcggaagtta tccgtcgtct ggccaactac gcccgtcagc gtggagatat
     5881 ggtggtgatt tatgaccttt caggggaatt tgttaaaagt tactatgatc cctccatcga
     5941 taaaatcctg aatccgctgg atgcccactg tgccgcctgg gatttatgga aggagtgtct
     6001 gacacagccg gattttgata atacggcaaa taccctgatc ccgatgggca caaaagagga
     6061 cccgttctgg cagggttcag gacgtaccat ttttgcggaa gcggcgtacc tgatgcgtaa
     6121 tgaccccaac cgcagctaca gcaaactggt ggacacactg ctttccatca aaatcgaaaa
     6181 actgcgtacc ttcctgcgta attcaccggc ggccaacctg gtggaagaga aaattgagaa
     6241 aacggcgatt tccatccgtg ctgtgctgac caactacgtg aaagccatcc gttacctgca
     6301 ggggattgag cataacggtg agtcttttac catccgtgac tggatgcggg gtgtccggga
     6361 agatcagaaa aacggctggc tgtttatctc gtcgaatgcc gacacccatg cctccctgaa
     6421 gccggtgatc tccatgtggc tgtccattgc cattcgtggt ctgctggcaa tgggagaaaa
     6481 ccgtaaccgt cgtgtgtggt ttttctgtga tgagttaccc acgttacaca aactgccgga
     6541 cctggtggag atcctgccgg aagcccgtaa gttcggtggc tgttatgtgt ttggtatcca
     6601 gtcctatgcc cagctggaag atatctacgg tgagaaagcg gctgccacgc tgtttgacgt
     6661 catgaacacc cgtgcctttt tccgttctcc cagccataag attgcagagt tcgctgccgg
     6721 tgagattggt gagaaagagc acctgaaagc cagcgagcag tactcctacg gtgctgatcc
     6781 ggtacgtgac ggggtatcga ccggtaagga tatggagcgc cagacgctgg tcagttattc
     6841 cgacattcag tctctgccgg atttgacctg ttatgtcacc ctgcccggac cgtatccggc
     6901 agtaaaactc tcactgaaat atcaggcacg tccgaaggtt gcccctgagt ttattccgcg
     6961 tgacatcaac ccggaaatgg agaaccgcct gagcgccgta cttgccgcaa gggaagcaga
     7021 aggtcgtcag atggccagcc tcttcgaacc ggatgtgccg gaagttgttt ccggagaaga
     7081 cgtgactcag gctgaacagc cgcaacagcc ggtgtctcct gccatcaacg ataagaagtc
     7141 agattcaggt gtgaatgttc cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga
     7201 agaagagatg gaacagcaac tgccacccgg gatcagtgaa tccggtgaag tggtggatat
     7261 ggccgcttat gaggcatggc aacaggaaaa tcatccggac atccagcagc agatgcagcg
     7321 tcgtgaagag gtgaacatta atgtgcaccg ggagcgcggg gaggatgttg agccgggaga
     7381 tgatttctga tgatgagtat cgcgcaggtc agatcggccg gaagtgccgg taactattat
     7441 accgacaagg ataattacta tgtgctgggc agcatgggag aacgctgggc cggcaggggg
     7501 gctgaacagc tgggactgca gggcagtgtc gataaggatg tttttacccg tcttctggag
     7561 ggaaggctgc cggacggagc ggatctaagc cgcatgcagg atggcagtaa caggcatcgt
     7621 cccggctacg acctgacctt ctccgctccc aaaagtgtct ccatgatggc catgttaggt
     7681 ggcgataagc gcctgattga tgcacataac caggccgtgg actttgctgt tcgtcaggtg
     7741 gaggcgctgg cctccacacg ggtgatgacg gacggacagt cagaaacggt gctgaccggt
     7801 aatctggtga tggcactgtt taaccacgac accagtcgcg atcaggaacc acagttacac
     7861 acgcatgcgg tggtggctaa tgtcacgcag cataacggcg aatggaagac actgagcagt
     7921 gacaaagtgg ggaaaacggg gttcattgag aatgtgtacg ctaatcagat tgcctttggc
     7981 aggctctacc gggaaaaact gaaagagcag gttgaggcgc tgggctatga aaccgaagtg
     8041 gtgggtaagc acggtatgtg ggagatgccg ggcgtaccgg tggaggcctt ttccggacgc
     8101 tcacaggcaa tccgggaggc cgtcggggaa gatgcctcgc tgaaatcccg ggatgtggcg
     8161 gcactggata cgcgaaaatc caaacagcac gtcgatcctg aagtcagaat gactgaatgg
     8221 atgcagacgc tgaaggaaac cgggttcgac atccgggcat atcgtgacgc ggcggatcag
     8281 cgtgcggaga cccgcacgca ggcgcccggg gctgtttcac aggaggggcc ggatgtgcag
     8341 caggcggtga cacaggccat tgccggatta agtgaacgaa aagtgcagtt tacgtacacg
     8401 gacgtgctgg ccagaacggt cggcatactg ccgccggaaa atggtgtgat tgaacgggca
     8461 cgcgccggta tcgatgaggc catcagccgt gagcagctta tccccctcga tcgtgagaag
     8521 gggctgttca cgtccgggat tcatgtgctc aatgagctgt cagtccgggc actcagtcgt
     8581 gacatcatga aacagaaccg ggtgaccgta catccggaga aaagtgtccc ccggacggct
     8641 ggttacagcg atgcggtcag tgttctggca caggaccgtc cgtcgctggc cattgtgtcc
     8701 gggcagggcg gtgcagccgg gcagcgtgag cgggtggctg aactggtcat gatggcccgg
     8761 gagcaggggc gggaggtgca gattatcgct gctgaccgtc gctcgcagat gaacctgaag
     8821 caggatgaac ggttgtccgg tgagctgata accggacgtc gtcagctgct ggaaggcatg
     8881 gccttcacgc cgggcagtac cgttatcgtt gaccagggcg aaaaactctc cctgaaagag
     8941 acgttaaccc tgctggacgg tgccgcacgt cataacgtac aggtcctgat aaccgacagc
     9001 gggcagcgaa ccggtacagg cagtgcgctg atggccatga aggatgccgg ggtgaacaca
     9061 tatcgctggc aggggggaga acagcgaccg gccaccatca tcagtgaacc ggaccgtaat
     9121 gtccgctatg cccggctggc cggagatttt gcggccagcg tgaaagccgg agaagagagc
     9181 gtggcacagg tcagcggagt acgggaacag gccatactga cacaggccat tcgcagtgag
     9241 ctgaaaacac agggcgtgct cggacaccag gaggtgacca tgacggcact gtcaccggtc
     9301 tggctggaca gccggagccg ttatctgcgg gatatgtacc gtccggggat ggtgatggag
     9361 cagtggaacc cggagacacg cagtcatgac cgctatgtta tcgaccgggt gacggcgcag
     9421 agtcacagcc tgaccctgcg ggatgcgcag ggtgaaacgc aggtggtgcg tatttcctcc
     9481 ctggacagca gctggtcgct gttccggccg gaaaaaatgc cggtggcaga cggcgagcga
     9541 ctgagggtga cagggaacat ttccggactc cgcgtctccg gcggtgaccg cctgcaggtg
     9601 gcatccgtca gtgaagatgc gatgacggtt gttgtaccgg ggcgggctga accggcctcc
     9661 ctgcctgtga gcgattcacc attcacggca ctgaagctgg agaacggctg ggtggaaacg
     9721 cccgggcatt ccgtcagcga cagtgcgaag gtttttgcct ccgtcacaca gatggcgatg
     9781 gacaacgcga ccctgaacgg tctggcccgc agtggtcgtg atgtccggct gtattcctca
     9841 ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc cctcctttac ggtggtttct
     9901 gagcagataa aggcgcgtgc cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa
     9961 gccgggcttc acacgccggc acagcaggcc attcatcttg cccttccggt ggtggaaagt
    10021 aaaaacctgg ccttcagcat ggtggacctg ctgacagagg caaagtcgtt tgctgcagaa
    10081 ggaaccagtt ttgctgacct gggacgggaa atcaatacgc agataaaacg cggtgattta
    10141 ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc tggtttcccg tgcgtcgtat
    10201 gaggcagaaa agagcattct tcgccatatt ctcgaaggta aggaggcggt cacgccgctg
    10261 atggagagag tacctggcga actcatggag aaactgacat caggacagcg tgccgccacc
    10321 cgcatgatac tggaaacgtc cgaccgtttc acggtggtgc agggctatgc cggtgtgggt
    10381 aagaccacac agttccgggc ggtgatgtca gccgtgaacc tgctgccgga gagtgagcgt
    10441 ccccgtgtcg ttgggctggg tcccacacac cgtgcggtcg gggagatgcg cagcgccggc
    10501 gtggatgcgc agacactggc gtcctttctg catgacacgc agctgcagca gcgcagcgga
    10561 gaaacgccgg atttcagcaa cacgctgttc ctgctcgatg agagctcaat ggtgggcaat
    10621 accgacatgg cacgggcata cgccctgatt gcggccggtg gcggtcgtgc cgtggccagt
    10681 ggtgacacgg accagctgca ggccatcgcg cccggtcagc ctttccgtct ccagcagacg
    10741 cgcagtgctg ccgatgtggc tatcatgaaa gagattgtgc gtcagacgcc ggaactgcgg
    10801 gaggcggtat acagcctgat taaccgggat gtggaaaggg cattgtccgg gcttgagagt
    10861 gtgaaaccgt ctcaggtgcc tcgtcaggag ggcgcatggg caccggagca ctctgtgacg
    10921 gagttcagtc acagccagga agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa
    10981 ggcgaggctt tcccggatgt ccccatgaca ctgtatgaag ccattgtccg cgactatacc
    11041 ggcaggacac cggaagcacg ggagcagacg ctgattgtca cacacctgaa tgaggaccgg
    11101 cgcgtactga acagcatgat tcatgatgca cgggaaaagg ccggtgagct ggggaaagag
    11161 caggtcatgg tgcctgtcct gaacacagcg aatatacgtg acggagaact gcgtcgtctc
    11221 tccacctggg agaataaccc ggatgccctt gccctggtgg atagtgtgta tcaccggatt
    11281 gccggtatca gcaaggatga cgggctgata accctggagg atgcggaagg taacacgcgg
    11341 ctgatttcgc cccgggaggc ggtggctgaa ggcgtcacac tgtacacccc ggacaccatc
    11401 cgggtgggaa ccggtgaccg gatgcgcttc acgaagagtg accgggagcg cggttatgtg
    11461 gccaacagcg tctggacggt gacagcggtt tccggtgaca gtgtcacgct gtcggacgga
    11521 cagcagaccc gggtgattcg ccccggtcag gagcgggcag agcaacatat tgacctggcc
    11581 tatgccatca ccgcccacgg tgcgcagggg gcaagtgaaa cctttgccat tgcgcttgaa
    11641 ggcacggaag gcgggcggaa acagatggcc ggctttgagt cggcctacgt ggccctgtcg
    11701 cgtatgaagc agcatgtgca ggtgtacacc gataaccgtc agggctggac ggatgccatc
    11761 aacaatgccg tacagaaagg aactgcccac gatgtgctgg agccggggtc tgaccgggag
    11821 gtcatgaatg cagagcggct gttcagtacg gcgcgggagc tgcgggacgt ggtggccggc
    11881 cgtgccgttc tccgtcaggc agggctggcc gggggagaca gtcctgcacg gtttattgca
    11941 cccgggcgca aatatccgca gccgtatgtg gcactgccgg cttttgaccg taacggcaag
    12001 tcagctggta tctggctgaa cccgctgacc acggatgacg gaaacgggct gcggggcttc
    12061 agtggtgaag gccgggtgaa aggcagcggg gatgcgcagt tcgtggccct gcagggcagc
    12121 cgtaacggag aaagcctgct ggctgataat atgcaggatg gtgtccggat tgcccgtgat
    12181 aatcctgaca gcggtgtggt ggtgagaatt gccggtgaag gtcgtccgtg gaatcccggt
    12241 gccatcaccg gtggtcgcgt gtggggggat attccggata acagcgttca gccgggtgcc
    12301 ggaaacggtg aaccggtcac ggcagaggta ctggcacagc ggcaggctga agaggccatc
    12361 cgccgtgaaa cggaacgccg cgcagatgaa attgtccgta aaatggcaga gaacaaacct
    12421 gacctgccgg acggcagaac agagcaggct gtcagggaga ttgccgggca ggagcgtgaa
    12481 cgggctgtca cttctgaacg ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa
    12541 cgggagcggg aggcggtccg tgaggttgtc cgggaaaacc tgctgcagga gcgactgcag
    12601 cagatggagc gggatatggt tcgtgacctg cagaaagaga aaaccctggg cggagactga
    12661 tacaggaaga taaacgctga tgacaacgga taacacgaac acgacacgta acgattcact
    12721 ggctgcccgg accgatacct ggttgcagtc gttgctggtc tggtcacccg gacagcggga
    12781 tatcatcaaa acggtggcac tggtgctgat ggtactggac catgcaaacc gcattctgca
    12841 tctggaccag acgtggatgt ttctggccgg gcgtggagcc tttccgttgt ttgctctggt
    12901 gtgggggctg aatctgtccc gccatgcgca tatcaggcag tcggccatta accggctgtg
    12961 gggatgggga attattgccc agttcgcgta ttatctggca ggctttccgt ggtatgaggg
    13021 gaatatcctg tttgcctttg cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag
    13081 tgggtggcgt acagcagcgg ccattctcct gatggcactg tgggggcctt tgtccggcac
    13141 cagttacggc attgccgggc tgctgatgct ggcagtcagt caccggctgt accgggcgga
    13201 agacagaacg gaacgtctgg tgctggttgc atgcctgctg gccgtgattc cggcacttaa
    13261 ccttgcctcc agtgatgcgg cggctgtagc tggtcttgtg atgacggtgc tgaccgttgg
    13321 tctggtgtcg cgtgtaggaa aatcattacc ccgtttctgg cccggagatt ttttcccgac
    13381 gttctatggc tgtcatctgg ctgtgctggg cattctggcg ttgtgactgg tatgatactg
    13441 acgctgattc tgcttattaa taaacaacat aacacaggat atttatgaaa attacggatc
    13501 acaaactctc tgaaggtatt gccctgacat ttcgtgttcc tgaaggaaat ataaaacatc
    13561 ctttgattat cttatgccat gggttttgcg gtatcaggaa tgttctgctc ccttgctttg
    13621 cgaatgcctt cacagaagca ggattcgcaa cgattacttt tgattatcga ggattcggtg
    13681 agagtgatgg agaacgaggg cgtctggttc ctgcaatgca gaccgaagat attatttcag
    13741 ttatcaattg ggcagaaaag caggaatgta ttgataatca gcgtattggg ttatggggaa
    13801 catcgctggg aggggggcat gtcttcagtg cagctgcgca ggaccaacgc gtaaaatgta
    13861 ttgtcagcca gctggctttt gctgacggtg atgttctggt taccggcgag atgaatgaat
    13921 cggagagagc gtcttttcta tccacgctga ataaaatggc agagaagaag aaaaacaccg
    13981 ggaaagagat gtttgtgggg gttacacggg tactaagtga tgatgaatcg aaggtattct
    14041 tcgagaaaat caaagcccgg catcccgaaa tggatataaa aatacctttc cttacggtca
    14101 tggaaacgtt gcaatataaa ccggcagaga gtgcagccag cgtccagtgc ccagttttgg
    14161 ttgttattgc cggccaggat agcgttaatc caccggaaca gggaagagca ttatatgatg
    14221 ctgttgcttc tggtactaag gaattatacg aggaagctga tgcatgccat tatgatattt
    14281 atgagggcgc gttttttgaa cgtgtagtag ctgtgcagac acaatggttt aaacaatatc
    14341 tttaattgtc agtatttata cgttaaaggt cgttgaaatt aacttcctgt tacgatgagt
    14401 tgtttcaacg gcctgcaggg catattttca cgaaggtcag agtaagcatg gcagagcaaa
    14461 aacgaccggt actgacactg aagcggaaaa cggaaggaga gacgcctgtc cgcagccgga
    14521 aaaccatcat caatgtcacc acgccaccaa aatggaaggt gaaaaagcag aagctggccg
    14581 agaaagcagc ccgggaagcg gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
    14641 ccatttatct gaacctgccc acgctggatg agaccgtgaa caccctgaaa ccctggtggc
    14701 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
    14761 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaagctgcgc agggcgctga
    14821 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
    14881 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcggcagcgc
    14941 gtctggataa aatccgccgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
    15001 agaaataaaa aaagcctccc tcagagagag gaggcaggga aataaggctt aaaaggaaat
    15061 aacttccgta cccgaaagga agctaattca gtagattatt taacaccata atactgacgg
    15121 gttaaagagg agtgataaag atgaatggat tcagaaacag ttcacggaac ggtcaggtct
    15181 ggcgttacca gcgtgccgga agccgggccg ttattctgga agtcagtgga cgctggatgg
    15241 aagcggcaga agcatggcga cgggctgccg gtgttgcacc ccggacagac tggcagcagt
    15301 ttgcccgaaa aagggctgaa cactgccacc ggcgctgccg aggaaggggg taacgaaaaa
    15361 agcagccttc attactccag ggattcagaa ggctgaataa tgcatgagat ttgctttttt
    15421 taatatccgg tcgggcagta tgaccggaca gttctgagta gcacagagtt ttttccgtat
    15481 taagtcgtga ttttatattt tgtgattaat ttcacaaaat aaggtgttat tcagtgtgtg
    15541 ctgcaatatt caggatgcct gaaatacagg tgctatttat ctgatacgga gaataacatg
    15601 aggaaatata ttccactggt attatttatc ttttcatggc cggtattgtg tgcagatatt
    15661 catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga ctcacggaag
    15721 gcggtgcgga tcaggctggt aaatattgat gcgccggaaa agaaacagga ttatggccgg
    15781 tggtcgacag acatgatgaa atcactggtt gcaggaaaaa cggttaccgt cacatatttt
    15841 cagcgagatc gttatggtcg tattctcggg caggtttatg caccagacgg aatgaatgtt
    15901 aatcagttta tggtccgggc cggggccgcc tgggtatatg agcagtacaa cacagatccc
    15961 gtgctgcctg tccttcagaa cgaagccaga caacaaaaac gtgggctctg gtcagatgct
    16021 gatccggttc ccccctggat atggagacac cgtaaataaa gcgatggcaa tcttatcatt
    16081 catctgttct cagcgggaat aaaaatggaa aaaacaaaac aggaatggct gtatcaatta
    16141 cggcgatgtt catcagtcaa tacgctggaa aaaattattc ataaaaacag agacagttta
    16201 tcgaccagtg aacgtgaatc atttaattca gcagcagacc accggttagc agaattaatc
    16261 accggaaagt tatatgacag gataccaaaa gaaatatgga aatatgtaag gtaataattc
    16321 agcataatta attaccagga gcagataaac aatgaagctg attattttta ttctcattgt
    16381 tctgattatt gcagcgttac ttatccggat tatcctcaga agtgtaaatc agcattctcc
    16441 tttgctgatg caattgcatg cggcaggtat acggaccggt gatgcagagc gaatactgtc
    16501 tgccgtctgg tttttcagga cggtttttcc agtatctgta gctgctgcga tgaaccccga
    16561 acacatggca gagtgtgacc acaggataat gcgctctgag tttcccgatt atcgagaact
    16621 gttcagggag tctgacatca agagcgcggt agcctttttt aatatttcat tctccatttc
    16681 aatgcgttgt agctttttcc tcagctcacg tatttcgatt tgttctggtg ttatcggaga
    16741 ggcttttggt gttttgccct gacgctcatc acgcagttgt ttgacccatc ttgtcattgt
    16801 ggaaaggccg atatccatag ctttggcggc atctgccacc gtgtagttct ggtcaacaac
    16861 cagttgagcg gattcgcgtt taaactctgc gctgaaattt ctttttttca ttggagcacc
    16921 tgtgttgttc tgaggtgagc atatcacctc tgttcaggtg gccaaattca gtgtgccact
    16981 acatctttcg aaaaccagtt ctgcggcttt tcaaagatgc catcatctct cttcacccgc
    17041 aggtactgga gttcctctgg tggtacaacc atttgcaatc gcgcttaaga tccagacctg
    17101 ggccaccgtt gtcatagagt gtggccttca agaattctaa gtcgccgggg ttcatgatgt
    17161 tccatttaca atgcaatagc ggacggacag tgtaaatttt taaaattgtt cgctgagcag
    17221 ctttatcaag ccatggatta tttgacgcgc cggtcaataa gctgaccatg cgagtgaaat
    17281 acggctgatt tctgcgggcg aattcggttg cgttttcagc gccccactgt ggtagcgtac
    17341 cgttcatctt cgcaatgtag aaacctctgc cgatttgtcc cgttggagga aggaagcgct
    17401 cctgaactcc gacggaaaga atattctggg ctgacgcctc actcgtgcca tgataaccga
    17461 tgaaatacat atttgatctc aaattattaa gttatattag aagtttatgc taatactcag
    17521 gagatatata ctaaaagcgc tcaatgttaa taaagtgtta tcacaagcta ctgcccacac
    17581 caagattgtt ggccgttttg gtaggtggac gacctcttgg actctcaata tatgagcaac
    17641 attcactcct tctctcctga gttttagttg aaatgtgccg caagaatatc agtgaacaat
    17701 gccaacaata tcatttttct atatattgct tatggcattt tcccgcaaag tttttaatat
    17761 tgagcaaaac tctctgtctg ccatcttatt tacaaataat ttgctatctt tgtcaattga
    17821 ataataatgc tcgtaactgt ttgaaaaatc aatcggccaa aaacgattat tcttttcatc
    17881 aaataagata tttcccatat gtaagtctcc atgaatgatt acactgtgac gttgtcgttg
    17941 agatatggtg ttaccgatag tcaggcatta tataattatg atctgatgac acgaaaaacc
    18001 attcttacat accgcaatca tatcagtgat gatcatatgc ctcttataga ctcagcatct
    18061 gccggaattt ctcacagtcc tctttctaat ttatattttt gcaaacagat atatttatct
    18121 tgcgtcaggt tctgaatata aatgttggtc ttgggagtga tatcccagca ggacatagcc
    18181 catcgatatt tgaggcgtgt ctccatgcaa taacagcaag taaagccctt aacgatgggg
    18241 gcaactctca gttatcttct gaggtctggg gatactccgg agcatctgtt tcattcaggg
    18301 aggctttctg gcttgcaaca ggcggtgatg gaaaaattct ggatttacca ataggaaaac
    18361 ttcggaaaga ttattttgtt gatactatag taattgatac taatggtcat aatttagata
    18421 tcataatata tgatgataca actgaggaca ttctgcagaa gttaataata taacacgcag
    18481 cgtaataata tatgcaaggt atgtgttaat ggtgagaata tttatcaaag aggtagtcag
    18541 agaagggtag aaatccttct ctgacagttt atcaataaca atgtttttag
//
LOCUS       DAZFYC010000093        18517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFYC010000093 DAZFYC010000000
VERSION     DAZFYC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.093"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..891)
                     /locus_tag="ACHKW5_003598"
                     /pseudo
     CDS             complement(1..891)
                     /locus_tag="ACHKW5_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1471..2472)
                     /locus_tag="ACHKW5_003599"
     CDS             complement(1471..2472)
                     /locus_tag="ACHKW5_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7512923.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            2580..2876
                     /locus_tag="ACHKW5_003600"
     CDS             2580..2876
                     /locus_tag="ACHKW5_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7512924.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2999..3424)
                     /gene="arsC"
                     /locus_tag="ACHKW5_003601"
     CDS             complement(2999..3424)
                     /gene="arsC"
                     /locus_tag="ACHKW5_003601"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO7512925.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(3437..4726)
                     /gene="arsB"
                     /locus_tag="ACHKW5_003602"
     CDS             complement(3437..4726)
                     /gene="arsB"
                     /locus_tag="ACHKW5_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO7512926.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(4780..5133)
                     /gene="arsR"
                     /locus_tag="ACHKW5_003603"
     CDS             complement(4780..5133)
                     /gene="arsR"
                     /locus_tag="ACHKW5_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO7512927.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            5642..5725
                     /gene="dinQ"
                     /locus_tag="ACHKW5_003604"
     CDS             5642..5725
                     /gene="dinQ"
                     /locus_tag="ACHKW5_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO7512928.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5779..7131)
                     /gene="gorA"
                     /locus_tag="ACHKW5_003605"
     CDS             complement(5779..7131)
                     /gene="gorA"
                     /locus_tag="ACHKW5_003605"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO7512929.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7203..8045)
                     /gene="rlmJ"
                     /locus_tag="ACHKW5_003606"
     CDS             complement(7203..8045)
                     /gene="rlmJ"
                     /locus_tag="ACHKW5_003606"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO7512930.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8248..10290
                     /gene="prlC"
                     /locus_tag="ACHKW5_003607"
     CDS             8248..10290
                     /gene="prlC"
                     /locus_tag="ACHKW5_003607"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO7512931.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10296..11048
                     /gene="rsmJ"
                     /locus_tag="ACHKW5_003608"
     CDS             10296..11048
                     /gene="rsmJ"
                     /locus_tag="ACHKW5_003608"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO7512932.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11097..12566)
                     /gene="dtpB"
                     /locus_tag="ACHKW5_003609"
     CDS             complement(11097..12566)
                     /gene="dtpB"
                     /locus_tag="ACHKW5_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO7512933.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            12732..12785
                     /locus_tag="ACHKW5_003610"
     CDS             12732..12785
                     /locus_tag="ACHKW5_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512934.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(12884..13318)
                     /gene="uspA"
                     /locus_tag="ACHKW5_003611"
     CDS             complement(12884..13318)
                     /gene="uspA"
                     /locus_tag="ACHKW5_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO7512935.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13709..14044
                     /gene="uspB"
                     /locus_tag="ACHKW5_003612"
     CDS             13709..14044
                     /gene="uspB"
                     /locus_tag="ACHKW5_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO7512936.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14115..15614)
                     /gene="pitA"
                     /locus_tag="ACHKW5_003613"
     CDS             complement(14115..15614)
                     /gene="pitA"
                     /locus_tag="ACHKW5_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO7512937.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15846..17048
                     /gene="yhiN"
                     /locus_tag="ACHKW5_003614"
     CDS             15846..17048
                     /gene="yhiN"
                     /locus_tag="ACHKW5_003614"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO7512938.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(17364..18416)
                     /gene="yhiM"
                     /locus_tag="ACHKW5_003615"
     CDS             complement(17364..18416)
                     /gene="yhiM"
                     /locus_tag="ACHKW5_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO7512939.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4849 a         4539 c         4691 g         4438 t
ORIGIN      
        1 tcgaagcaat ttccgctttt gctcgtaaaa agttgtcatt aatggttaat aatgttgaaa
       61 ataaaccttc gatttgtttg tcaagatatt gtccatcttt atataatttg ggggtagggt
      121 gttctttatg taaaaaatag tgtcacctaa atttgcgtca gtgaaattta tattaagtag
      181 atcttcgaaa taaaaccttg acaaggttag aacgaacccg gacaggttct gtcctgtcat
      241 attgaaatca ataaagttgg ttccggcaaa gactttcatc ctgtttttat tttcactgtg
      301 ctcaatatat aacttcgtca ttttttgata caggctttga atatctttta tattagttgg
      361 taagtcagct cgttgttcag caataaaatt agtatctgaa aacatggttt ggacactgaa
      421 tgttatgggt attccctctt ctacgctaat tttagttatg attgccttgt cgggtttagg
      481 attagcgaca acaatgattc gacattcagc acttttgttt ttttcatatc taatgttatt
      541 aatctgatta agtattgatt gtgtctgttg cgtaagtggt gtcgaactta tcgtttcaat
      601 gttattaata agacttacaa gttgaaccac aacttttgag tagtctgcat ttgtatatgt
      661 ggtgttcgtg tggcatgcat taatttgttg gagttgttgt tgcgctaaat caaattgttc
      721 agggttgtat ccttctccag agaatatgga attaatgggt tgttgttctt cccaattatc
      781 acaaggccac ttcggtgtgt ctggcgtttt aacattatcg gtatttgttg tgcagttata
      841 aatatcgccg tgatatgtat ttattatacc tggggtaaag tcaattgaca taacattcct
      901 tattcatcat ctgttttatg ctaaggtttt taaattgaat aattagaaca ggaatggatt
      961 ttcttctaca cacaataaat ataatgcgtt atatcttcct gatataatta cctttaaatt
     1021 ccctttcatt tccacccggt aatatttgtg tataaaaaaa ggcagtgata gtatcgtaaa
     1081 ggaaaacgtt aacaactatc aaaaagcgct cattttaaaa agttattatg ttctccgata
     1141 gtgtgtgaag taatattttt gtgatgtttt agatgtgtaa taatgaacca ggttaaagtg
     1201 tatttaaaaa tgtgttgtat tagcgactga aatctgcctt ttaaaaagga ggagggtact
     1261 gagacaaaga gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt
     1321 gtctgttgat gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt
     1381 gtgattttta aggattatcc tgtctatgat tttctactta gaaggatatg gcactaacta
     1441 tgcatcttat agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat
     1501 caagatagcc atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat
     1561 tgatttaagt aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat
     1621 taatgctccc agacccattc cttttgtcat cagaacagat gccagaggga taactgcctc
     1681 agcccggata tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc
     1741 tgctcctgca tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat
     1801 taatactcct aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt
     1861 ggctagtttt atgggattaa tacttactac agctttggta ctgcaacagg taactacggg
     1921 gttctcaatg gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt
     1981 tcccggcgaa gtttttgcta gagcacaaca atttgctgat gagcttttac tggcaatgat
     2041 atgacgttca aaccccagag aatccaggat aatactggca agaacggaga cgccggcggc
     2101 gataatcgcg tacaacaagg tgactttcca accaaaggtc atccacatta gtccgacgat
     2161 aatgggatta agtaatggtg aaacaaataa aaaagtgaga gtcggaccaa acccggcttt
     2221 cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg gggtaacggc
     2281 tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca tctgctggat
     2341 tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca ctataaacag
     2401 caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca gcatcgcaag
     2461 ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa tcgaaatgaa
     2521 attcagatca agatatttct ggtattatcg aaatatcaac cagacaaagg gggtgtcata
     2581 tgcagttgga agaagttgct aaagcgctga aagagctagg tcacccgacc cgtttgttca
     2641 tattcaaaca cctggtcaaa gcgggggagc aaggtttgcc ggtcggtgag ttgcaaaagc
     2701 agcttgggat acccggctct acactcagtc accatatttc agcactggta tcggtagggc
     2761 tggttaaaca aaaccgtgag agtcggacgc tgatgtgtgt ttcgcaatac gcaatgctgg
     2821 aagcgataat tgaattctta cgcgaggagt gctgcgtaaa tagcaaaatt gcctgatgcg
     2881 ctacgcgcta cagatttcat gccatgcacg gttttgtagg tcggataaga cgcgttagcg
     2941 tcgcatccga cataaacaaa gcgcacttta ccaactatgg gcagtggata tcgccgcttt
     3001 atttcaaccg cttacccgct tcatcaacca ctttctcgcc atcttccttg gagaatgcgc
     3061 ctttttgcgc atctggcaga atttccagca ccacttctga agggcggcac aggcgagttc
     3121 ccagcggcgt caccacaatc gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga
     3181 ttaaccgatc gtcagtaaat ttatcttctg caaggcccag ttcctcatac ggctcgacgt
     3241 ttttacgcaa cagcgcgcgt acggaaatcc ccatatcggc aatgagtttg accagctcat
     3301 cgcgcgttgg cggtgtttcc agataataga taatggtcgg ttctgtaccg ctgttgcgga
     3361 tcatctccag cgtattacgc gacgtaccgc aggccgggtt gtgataaatg gtaatgttgc
     3421 tcatatcaat atctcattac aaagtgaaag agagacgtag cgccagcgca gccagcgtca
     3481 caaaaagcac cggcagggtc atgataatcc ctgtacggaa gtaatatccc cagctgatag
     3541 tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt agccaggcta ccaattgggg
     3601 taattttcgg tcccaaatcg cagccaatca cattggcata aaccatcgct tctttgataa
     3661 cgccagatgc cgtgctgcca tcaatggaca acgcgccaac cagtaccgtc ggcatattgt
     3721 tcataataga agagaggaag gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc
     3781 cgttatccgc cagcacgttg agtacgccag aaagatattc cgttaatccg gcattgcgca
     3841 ggccataaac caccagatac atgccgagcg agaagatgac aatctgccag ggggcaccgc
     3901 gcaggacttt acccgtatta atcgcatgac cgcgtttagc gacgacaaat aatatcagcg
     3961 cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca
     4021 ccagcagaag cagtaaaaca acccagccag ttttgaacgt agcaggatct ttgatcgctt
     4081 ctgcgggaga tttcagcagc gccatatcgt agttctgcgg aatatctttg cgaaaataga
     4141 gatgtaacat caccagcgtg gcaacaatcg cggcgatatc caccggcacc atcaccgagg
     4201 cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac gatattcacc aggttggaga
     4261 caataagcgg caggctggcg gtatcggcaa tgaatccggc cgccatcacg aacgccagcg
     4321 tagtgccttt actgaaccct aaagccagca gcatggcgat gacaatcggt gtcaaaataa
     4381 gcgccgcgcc atcattggca aacagggcgg caacggcagc accgagcagg acaatccagg
     4441 taaacagcaa gcgaccacga ccattacccc agcgtgagac gtgcagcgcc gcccattcaa
     4501 aaaagccgga ctcatccagc agcaggctga tgataatgac ggcgataaac gcagccgtcg
     4561 cgttccagac gatattccac accaccggaa tatcacccgg atggaccacg cccgtaacta
     4621 acgccagtac tgcgccgagc gttgcactcc agccgatgcc taaacctttc ggctgccaga
     4681 taaccaatac gatggtcagg acaaagatag cgcctgccag taacataatg cctcccggat
     4741 aaaacacatc tgaaaattca tatgtgttta gctaaatttt taactgcaaa tgttcttact
     4801 gtccccggaa cagttttgtc gagccaggtt gcggacaatc gcctgaacct tttcctgttc
     4861 acatcgccag gcctcatcaa taattttcgc cgcccatgct ggaatatgcg gtgataagcg
     4921 gtaatgaacc cacttacctt gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc
     4981 caggtggcgg gagatcttgg gctgcgactg gtcgagagca gtgcagagat cgcagacgca
     5041 taactctccc agttcgctga gcagtaaaac gatgcccaga cgggtttcat cagcaagaat
     5101 tttgaacaat tggatgggta acagaaatga catattgcgc tcctgattgt tgcaggtagt
     5161 gtctctcttc gaagcggata agtcaaaaac atatatgact taacgaatgt gtaagtgcag
     5221 aggaaggtaa taggtgtgaa ttttgagttg gctattcatt tgaaaggagg tctgaatcag
     5281 gacgtaaaaa aaccgacttt gcgtcggttt tttactttcc agccctgagt tggtggcact
     5341 ggctggagtg agaatgccat tatctaacgc atcaatgcta ataccatact gaaactattg
     5401 caaggacatg ctggttttat aacctgaatg tactgtatga ttatccagtt agctctgagg
     5461 cattttcact ctggtaatgc gcataaacgc tttcaaagtc ctggtcagaa gtacgggtgg
     5521 tgccgttaac tgatgctctg gccggagtga gaatgccatt atctaacaat agggcatgcg
     5581 ccgtgacagg cagtggatga gtaagcggat gcattctcac tccatcgcat ggagaaaacg
     5641 ggtgattgat aaagcaatca tcgttcttgg ggcgttaatt gcgctgctgg aactgatccg
     5701 ctttctgctt cagcttctga actgatagcg gaaacgtaat taagggctaa gagcacacta
     5761 ctcttagccc tttaacattt aacgcattgt cacgaactct tctgccgccg ttgggtgaat
     5821 ggcgacggta ttgtcgaagt cttttttggt tgcacccatc ttcagcgcca ccgcgaagcc
     5881 ctgcaacatt tcgtccatac caaagccaat gccgtgaata ccgacaatct tctcttccga
     5941 tccaacgcac accagcttca tgcggcacgg ctggcggtga gtggtgacgg cggtatacat
     6001 cgcggtgaaa gaggatttat acactttcac ctgatcgtcg ccatactgct cgcgcgcctg
     6061 cggttccgtt aaaccaacag taccaatcgg cggatggctg aagaccacgg tcggaatgtt
     6121 gctgtaatcc agatgctcat ccggcttgtt attaaacagg cgttcagaga gacggcgacc
     6181 cgctgcaact gccaccggtg tcagctccac tgcacccgtg ttatcgccca ccgcgtaaat
     6241 accttcaata ttggtgtttt gatatttatc gacgacgata tagccttttt cgttagtttt
     6301 aacgccagcg gcttccaggt tgatgttgtc attggcaggc tcgcgaccaa tcgcccaaat
     6361 caggcaatcc accgtttcac tgcgaccatc ttccagctcc agcgtcaggc taccatcggc
     6421 atttttcact accgctttcg ggatggcgtt ggtgtgcagc tgcgggcctt cggcgttcat
     6481 cacttcgacc agcgtttcgg aaatcatcgg gtcgaagctg cgcagcggcg catgtttacg
     6541 cacaaacaga tgcgttttcg cgccgaggcc gttaatcacg cccgccagct caacggcgat
     6601 gtaacccgcg ccaacaaccg ccacgcgctc tggcaaagca ggaagggcga agaagccatc
     6661 agaatcaata ccgtattcca cgcccggaat atccgggtgg ctcggacgac cgcctgtggc
     6721 gatcagaata tgatcggccg tgatggtttc gccgtttacc tccagcgttt tggcatcaac
     6781 gaagcgggca aagcctttga ttacatcaac gttattttta ccgagcacgt tttcatagga
     6841 agtatgaata cggtcgatat aggcggtacg gctggcgatc aacgtttccc agttgaattt
     6901 attgatagtg gtatcaaaac cataatccgg gccgtacata tggatcgctt cacggatttg
     6961 cgccgcgtgc cacatcactt ttttcggcac acagccgacg ttcacgcagg tgccgcccag
     7021 ctctttggct tcaatcagtg cgcatttctg gccgtacata gccgcgcggt tgatggaggc
     7081 tataccgccg ctgccgccgc cgatggcgat gtaatcatag tgtttagtca tgacagagtg
     7141 tccttatcgt tgattaccgc gattgtagcg cgagacgtaa taggtgccag caatggctgc
     7201 aattactccg gcacgatcca gcttacggtg gcgtgcccgg tgcctgccgg aaccagtttg
     7261 ctgtgcagcc acggcagcac gttattcatc tgttgttcca gtttccacgg cgggttaatc
     7321 acaatcatgc cggaagcggt catgccacgg cgatcgctgt ctggcagtac cgccagttca
     7381 atttgcagaa ttttgcgaat accggtcgct tccagatcgt ggatcatgcg cttaatttgc
     7441 tgacgcagca ccaccggata ccacagtgcg taagtaccag tggcgaaacg tttgtaacct
     7501 tctgctatcc cgctgaccac cgcctgatag tcggttttca tttcatacgg cgggtcgata
     7561 aggattaaac cacggcggga aaccggcggc agtttggcct taagctgctg gaaaccatcg
     7621 gctttttcga cgcgcgcacg gctgtctttc tgaaattcag aacgcagcaa cgggtaatcg
     7681 ctcgggtgca gttcggtcag ttgcaggctg tcttgttcgc gcagtagctg gcgagcaatc
     7741 aacggcgaac cggggtagta acgcaactga ccgctacggt tgaagtgttt taccacattg
     7801 atgtacgcct ccagttctgc gggcaaatcg tcctgctgcc agatacgggc gatgccttcg
     7861 agatattcgc cggtacgctc ggcatgttcg ctgcctaact gataacgccc ggcccctgcg
     7921 tgggtgtcga gatagagaaa cggtttatct ttctctttca gcgactcgat gatcaggctc
     7981 tgaacggtat gtttaaggac gtcggcgtgg ttgccagcgt gaaagctgtg gcgataactg
     8041 agcatgggta aaggtgttcc ggtaagtaaa aatcggcccg tattcggggc gcacaaaagc
     8101 gtatcaggac agtataccga aaagaggccg ccgccgcgaa agcgtaacgt ttctcattga
     8161 aattcactac acttaacccc atgctacaca cattatgtaa agcgcctgtt gagcgcttcc
     8221 ttaacctctt taaccaggac tgcgcgaatg acgaatccgt tactgactcc ctttgaattg
     8281 cctccgtttt ctaaaattct cccggaacat gtcgttccag ccgtgactaa ggcattgaac
     8341 gactgccgcg aaaacgtgga gcgcgtagta gcgcaagggg caccgtacac ctgggaaaat
     8401 ctctgccagc cgttggcgga agtggacgat gtgttggggc gtatcttctc cccggtcagc
     8461 cacctgaact cggtgaaaaa tagcccggaa ctgcgtgaag cgtacgaaca aaccctgccg
     8521 ctgctgtcgg aatacagcac ctgggtaggg caacatgaag ggctgtataa agcgtaccgc
     8581 gacctgcgcg atggcgatca ttacgccacg ctgaacacgg cgcagaagaa agcggttgat
     8641 aacgccctgc gtgattttga actctctggc ataggtctgc cgaaagagaa acagcagcgt
     8701 tacggcgaaa ttgctacccg tctttctgaa ctgggcaacc agtacagcaa caacgtcctc
     8761 gatgcgacaa tgggctggac caaactcgtt accgacgaag cggaactggc ggggatgcca
     8821 gaaagcgcgc tggctgcggc aaaagcccag gcggaagcga aagaacttga aggctacctg
     8881 ctgacgctgg atatcccaag ctacctgccg gtaatgacct actgcgacaa ccaggctctg
     8941 cgtgaagaga tgtatcgcgc ttacagcacc cgcgcctccg atcaaggtcc gaacgccggt
     9001 aagtgggaca acagcaaggt gatggaagag atcctcgcgc tgcgtcacga actggcgcaa
     9061 ctgctgggct ttgaaaacta cgcctttaaa tcccttgcca ctaaaatggc agaaaacccg
     9121 cagcaggtgc tggatttctt aaccgatctg gcaaaacgcg cgcgtccgca aggcgaaaaa
     9181 gagctggcac aactgcgcgc ctttgccaaa gccgaatttg gcgtcgatga gttgcagccg
     9241 tgggatatcg cgtactacag cgaaaaacaa aaacagcacc tctacagcat cagtgacgaa
     9301 cagctgcgtc cgtacttccc ggaaaacaaa gcggttaacg gtttgtttga agtggtgaaa
     9361 cgtatttacg gcatcaccgc taaagagcgt aaagatgttg atgtctggca cccggatgta
     9421 cgtttcttcg aactgtatga cgagaacaac gaactgcgcg gtagcttcta cctcgacctg
     9481 tatgcccgtg aaaacaagcg cggcggggcg tggatggatg actgcgtagg ccagatgcgc
     9541 aaagctgacg gttctctgca aaaaccggtc gcgtatctga cctgtaactt taaccgcccg
     9601 gtaaatggta aaccggcgct gtttacccat gacgaagtga tcaccctgtt ccacgagttc
     9661 ggtcatggtc tgcatcatat gctgacccgc atcgaaaccg ccggtgtatc cgggatcagt
     9721 ggggtgccgt gggatgcggt cgaactgccg agtcagttta tggaaaactg gtgctgggag
     9781 cctgaggcgc tggcgtttat ctctggtcac tacgaaaccg gcgaaccgct gccgaaagag
     9841 ttgctggata aaatgctggc ggcgaagaac taccaggcgg cgctgtttat tctgcgccag
     9901 ctggagttcg gcctgttcga tttccgcctc catgccgagt tccgcccgga tcagggagcg
     9961 aaaatcctcg aaactctggc agaaatcaag aaactggttg ccgtagtacc gtctccatcc
    10021 tggggccgtt tcccgcacgc tttcagccat attttcgccg gtggttatgc cgcaggttac
    10081 tacagctacc tgtgggctga cgtactggcg gcagatgctt tctcgcgctt tgaggaagag
    10141 ggcattttca accgtgaaac cgggcagtcg ttcctcgaca acattctgag ccgtggcggt
    10201 tcagaggagc cgatggatct gttcaaacgc ttccgtggtc gtgagccgca gctggatgcg
    10261 atgctggagc attacgggat caaaggttag tttttgtgca gatttgctta attgatgaaa
    10321 caggcaccgg agacggtgcc ttatctgttc tggcggcccg ctgggggctg gagcacgatg
    10381 aagacaacct gatggcgctg gtgttaacgc cggaacatct ggaattgcgc aagcgtgatg
    10441 agccaaaact tggcggcatc tttgttgatt ttgttggcgg agcgatggcg caccgacgca
    10501 aattcggcgg tggtcgcggt gaggcggtgg cgaaagcggt gggcattaaa ggcgattatt
    10561 tgccggatgt ggtggacgcc accgcagggc tggggcgcga tgcctttgtg ctggcttcag
    10621 tcggttgccg cgtgcggatg ctggagcgta atccagtcgt tgccgccttg ctcgacgacg
    10681 gcctggcgcg tggttatgcg gatgcggaaa tcggcggctg gttgcaggag cggttgcagt
    10741 taattcacgc ctccagcctg acggcgctga ctgatattac cccgcgcccg caggtggttt
    10801 atctcgaccc gatgttcccg cataagcaga aaagcgcgct ggtgaaaaaa gagatgcgtg
    10861 tctttcagtc gctggtgggg ccggatcttg atgccgatgg attgctggag cctgcgcggc
    10921 tgctggcgac caaacgcgtg gtggtaaagc gcccggacta cgcgccaccg ctggcgaatg
    10981 tcgccacgcc aaacgcggta gtcactaaag ggcataggtt tgatatttat gccggaacgc
    11041 cggtgtaaaa gtaaagcagg gtagcgataa cgctaccctg ctttctgcaa gaataattaa
    11101 tggctttccg gcgtcgcaat catgcgtttc agccacggca ccatcaacag catcactact
    11161 gcaacgccca gcgtgaccag accaatctta ccaaacacgt tggtatagac gggcaacgtc
    11221 tcaagcggat cggtaatgtt gtccggcacc gcggtaaatg ttgccacata gccgcccagc
    11281 aagaacgcgg cagcctgcgt caggaaccac atcccgagaa taaagcccat caaatgctgc
    11341 ggcaccaggg cagcaatcat cgccagaccc agggcgctaa taaacaattc acccaggctc
    11401 tggaacaagt aaaccagcac gataaaccac ggcgatgtca gtccttgtgc atcagcaaac
    11461 cacattcccg cagctgccgc cgtcaaaaag cccagtgagc acataaacat gccgagagta
    11521 aatttcatcg gcatcgagag gtctttgcct ttgttaccca gatgcgtgta aatgcctgcc
    11581 agtattgggc tggcgagtac cacccagaac gggttaagcg cctggaagct gaccgggttg
    11641 atggaaaagc cgagaatttc atgatgcacg ttgttgatgg caaagaagtt cagcgacgtt
    11701 ggcatctggg cgtagagaat gtaaaacacc accgcttcga gcatcaggac aaaggcgaca
    11761 aacattttat tgcgcccggt tttatccagc ttgaatgcct gacgaaagaa gatgatggtg
    11821 acgacgatgg agagaacaat cagcaccaga ttggcgactt ctacgttgtg catcagccat
    11881 gcacagacga agatcatcac cacgctgcca agtaacacgt acaacagttt gctgaagctc
    11941 attggccgga agtcgggttc agaaccaatg tctttcacca ttccacgaca ggcgatgtaa
    12001 accagtaatg cgataattaa ccccgccccg cacaggttgt aggtgactga ataaccgaat
    12061 ctatcagcga tcacaggggc cagcgataac gctatcaacg agccgatatt gatcgacata
    12121 tagaacaggg tgaatgcgcc atcaagccgc ggatctttcg gcggatagca cttcgaaagc
    12181 aagctggctg ggttagcttt aaacaggccg ttaccgacag cgatagtccc cagggcgatg
    12241 aaaatcaggt caggcttaag tagcgacatg ccggtcatga agtagccaat cgccagcaca
    12301 agtgctccga gaacaatggt gcgtttggtc cccagcaggt ggtcgccgac atagccgcca
    12361 atggaaatga ggccatagac cagcgcagca aaagcaccaa aagtgacaaa agcctgctct
    12421 tgcgagaatc caagctgttt aacgaagaaa accgccagta cgccctgcac gccgtagtag
    12481 ccgaatcgct cccataactc gacaaaaaag atcatgaaaa atgggcgagg ttgctgcagc
    12541 atccccatgg gtgttgttgt attcatattt attaaccttc caataccatc ctgaaaagta
    12601 tgaacgcgta agggataacg cgcgaaaaag tttacatctg tcagagattc ttaatattga
    12661 tgtggtgaat agattaatac actattacaa cagaaaataa ccagctgatc acactaatga
    12721 tggaaaatat gatggtgtta aatgttgtat tggcaggaga ttttgattgc gtacgcagat
    12781 cgtaggccag ataaggggtt tacgctgcat caggtaacaa ggcgcgccaa aaagcaaaac
    12841 gcccgcatat gcgggcgttc aggaacacgt cgtgggggga tgattattct tcttcgtcgc
    12901 gcagcggaac aatcagcata tcaacgtgaa cggtgttgat cagctgacgt gcggaagaca
    12961 ttagtttgct ccagaagtcc tggtggtgac cacaaaccac cagatccata tcgtatttct
    13021 tgattgcatc gaccagaacc tggcccaggt cgccgctgcc gctcagggtt tcagtgattg
    13081 ggtagcctgc attagtggaa agctcggtca gcgcatgatg tgtctcttca gagatgcgtt
    13141 tctgcatatc acccagattc acatcaataa gcccggtgta taggtcagag tagtttacat
    13201 ctacgtggat cagagaaact ttcgcattgt aggggcgagc catagagact gctttctcta
    13261 ccagaacttt gctttccggg gagagatcga ccgcgatgag aatgtgttta taagccatag
    13321 tgttactcct tccataaagt tgtcgatgac tggccagcta gcgtttcttg tgccgttacg
    13381 tcacccgcgt cctgcgaact acgtgcgcgg ggctaaccca gccgtaaaga ttcagtgtta
    13441 agaccatcct taccttatag cgacccggat gatccgtcaa tccgccttgc ttacccgtta
    13501 gataaacaaa ttttcgacat gaatatattg atagtggtta accttctgga aaaaaaacaa
    13561 cctgatctcc tacactatct atagagccgc tcggatgttg gtcaccacaa aagcggtttc
    13621 tccaggacct ggtgttaact gattggcagc gtatcgggag cggtagcccc ggggtggaac
    13681 tccgcgggca ggtcgccggg gaggaggtat gataagcacc gtcgcattat tttgggcttt
    13741 atgtgtcgtt tgcattgtta acatggcgcg ctatttctca tcactacgcg cgttgttagt
    13801 ggtactgcgt aactgcgatc cattgctcta tcaatatgtt gatggagggg gcttttttac
    13861 ctcacatggc caacccaaca aacaggtgcg tctcgtttgg tatatctatg cccaacgtta
    13921 tcgcgatcat cacgatgatg aatttattcg ccgctgtgag cgggtgcgtc ggcagtttat
    13981 tctgaccagc gcattgtgtg gtctggtggt ggtcagcctg attgcattga tgatttggca
    14041 ttaagcactt cagcaataat aacgcataaa aaaagcgggc cagtaaactg acccgctcgt
    14101 tttggtgtgt acgattacag gaactgcaag gagagccagt acagtccgcc ggaaagcagt
    14161 accgcagccg gaagggtaaa cacccaggcc atcagaatac tggttacggt tttacgctgt
    14221 aagccgccgc catctaccac catcgtcccc gcgacagaag aggagagtac gtgagtagtg
    14281 gaaaccggca tcccggtata actcgccagg ccgatagaca ctgccgccgt catctgggca
    14341 gacatcccct gagcgtaggt catgcctttc ttaccgattt tctcaccgat agtcgttgcc
    14401 acacggcgcc agccaatcat cgtaccgata cctaacgcca gcgcgaccgc catgatgatc
    14461 cacaccggtg catactcgat ggtgctaagc atgtcggact tcagtttctt caacaggcgc
    14521 tgatcgtcag cactcacgcc aggcatcttc accactttgt cgatagtgtc agaaacgcac
    14581 agcataatgc ggcgcatctg gctacgttga tcaagcgaca gcttgtcgta gctttccaca
    14641 tcggtggtca acataccttt caggcggttg agcgcgttaa tggtattcga cggatggcag
    14701 tggaactccg caggttgcgt tgcgccagct tccggagccg gtactaactg atcagcaccg
    14761 gtagcctgtt tgagcagcgc aggatgctgc tcaaagtaag cttcgacgtt gttgatggca
    14821 tcacgggtac gggtgatttc gtagccagtg gcattcatgt tcaccacgaa gcctgctggc
    14881 gcgacgccaa tcaataccaa cataaccaga ccaatgcctt tctgaccatc gttcgcgccg
    14941 tgcgaaaacg ccacgccgat agcggaaagg atcagcgcga tacgcgtcca gaacggcggc
    15001 tttttcttgc cgtctttctt ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc
    15061 ttggtgccgc tccagtagcg acgcagcaag aaaatcagac cgccagcaaa caccaggccg
    15121 acaatagggg aaacgatcag agaaccgaaa atacttaata ctttcgggat attgagtgca
    15181 tccaccactg acgtcccggt catcaacgca ttggttaaac caatcccgat gatcgcgcca
    15241 atcagcgtat gagagctgga tgcaggtaaa ccaaagtacc aggtacccag gttccagata
    15301 atcgccgcca gcaacataga gaacaccatg gcaaggccat gagacgatcc catattaagc
    15361 agcagatccg tcggcagcat atgcacaatg gcataggcaa cactcagacc acccagcaaa
    15421 acacccaaaa agttgaacac cgccgccata accacggcga gctgagaacg catcgcgcgg
    15481 gtatagataa cggttgccac ggcgttggct gtgtcatgga aaccattgat ggcttcgtag
    15541 aacagcacaa aagccagtgc aagcaataat aacagcccgg tatgcaaatc caggccagca
    15601 aacaaatgta gcataggacg ttacgccatt ttgaggacat gaacgcggcg cattatcagt
    15661 gactttcgcg gcgcgggcaa agtgaaatat agactttttt tgatttgcct cctgtatgga
    15721 tttcactcaa aaaataatta tcttatataa ttcaggcaaa tacttccttt tagtaatatt
    15781 gatgctggtg cgaccactga ggaatcttta caattcacgc ccgttttttc taagaggagc
    15841 gcaacgtgga aaggtttgat gccattatta taggcgctgg tgcggcgggt atgttctgtt
    15901 ctgcgctggc aggtcaggca ggacgccggg ttctgctgat cgataatggt aaaaaaccag
    15961 ggcgcaaaat ccttatgtct ggcggtgggc gctgcaactt taccaacctt tatgtcgaac
    16021 caggcgctta tctgagccag aatccgcatt tttgtaagtc tgcactcgcg cgttttaccc
    16081 agtgggattt cattgatctg gtcaataaac acggcatcgc ctggcacgag aaaacgttag
    16141 gacaactctt ctgcgatgac tccgcgcagc agattgtcga catgctggtg gatgagtgcg
    16201 agaagggcaa tgtaaccttc agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa
    16261 caggcttcac gcttgaactg aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga
    16321 ccggtgggct gtcaatgccg gggctgggcg cgtcgccgtt tggttataag attgccgaac
    16381 aatttggcct caacgtgctg ccgacccgcg cgggtctggt gccattcact ctgcataaac
    16441 cgttgctcga agagttacag gtgctggcgg gcgtggcggt gccttccgtg attaccgctg
    16501 aaaacggcat cgttttccgt gagaacttac tcttcaccca tcgcggcttg tctggaccgg
    16561 cggtgttgca gatttccagc tactggcaac cgggtgaatt tgtcagtatc aatctgctac
    16621 cggatgtgga tctcgaaacc ttcctgaatg agcagcgtaa cgcacatccg aatcagagcc
    16681 tgaaaaacac actggcggtt catctaccga agcggttggt tgaacgctta cagcaactcg
    16741 ggcaaatccc ggatgtttcg ctaaaacagc tcaacgtgcg tgaccaacag gcactgatta
    16801 gcacattgac cgactggcgc gtacaaccca acggcactga aggctatcgc actgccgaag
    16861 tgacgctcgg cggcgtggac accaacgaac tctcttcacg gacgatggaa gcgcgcaaag
    16921 tgcctgggct gtacttcatc ggcgaagtga tggacgttac cggctggctg gggggctata
    16981 acttccagtg ggcgtggtcg agtgcgtggg cttgtgcgca ggatttgatt gcagcgaagt
    17041 cgtcctgacc tgttacctaa agatgaaaat tattagagcc gtattaaaaa tgagctaatt
    17101 ttgatagtgg ctatcttgtg attattttct aatgagcccg tgaactgaaa ccctccaggc
    17161 ttaatataag gtggaaggaa aggtgattga aaactcaccc agtggccaga actttataaa
    17221 atatgaacag gttgaaaaaa acagtaactt tgttgttttt ttatccttaa ccactatgca
    17281 ttaatgctgc gttatttcat gatgcctaag aaaaaccagc gttacgcaaa tggtcaacgc
    17341 tggttttatc cggtacgttg caattatttt ttagcagaac ctgcttctaa tattgaaacg
    17401 attgagaaca acgtaaagca taccgctcct aaaccgacca aaacccgcga aggaataaaa
    17461 tatcccatgt cggtctgcgc catttccgca agaaacgatg ccaggaaaag gcaaaacagg
    17521 caggtgaaga cgggaatcat cggtattctg ttggctaacg aacaggtacg tctccatacc
    17581 agtgccagta gccagacttt tgagaatatg ctgtaacaga tcattccgag gcaaataaga
    17641 ataatgccgg gagccagtcg ggcgcttgca tcggaactga ctaagacgta tatcccctgc
    17701 agtaccgtga ttgagccgag aaaaataacc caataacacc acagccagtg ttctttagtt
    17761 gaaaacgtat tacgtgtttg atggacaatt gtggcaacaa ggccaattag acaggcacag
    17821 attgcggtta acccaagcaa tacgtgaccc gcgacataat gcggagttat gtcagcacta
    17881 cgtaacagcg taatagacca aatgaaaccg agcagggtga gtaaaacggg aacggcaatg
    17941 aggcaattac cgattaatga agaataagcc tgtaccggcg ttccatcgct cttgctcccc
    18001 gctgcatttt tgggaatgag cagaaagtga ccggatgacg ctgccaccgt cgatacacag
    18061 gcggcaatca taccaacgcc gaaaataaca tggccggcga caaactcgtc tgccatcaca
    18121 tcattgcctg ctaacagtgc ccaaccccat atcatggtga taattgaccc cgcatagcca
    18181 ataatgggga acaatgtatt gtaaaacgta ttaacgccgc gcgtgagctg cgaaatgata
    18241 ataaatgcgg tagtgaataa tgccaggcat atggccgcca gagaaatcag cacatgaccc
    18301 gcgacgaaat actcgctttg ccctgaactt tccagcacaa agccacctaa cgcgatgcaa
    18361 attaatccca taatcaaagg gattaatttg aaaagccacc cgatatatat gttcacttta
    18421 aactcctcag gtatccctgc taataatgaa atgtgcatta atgataattt cagcctgtta
    18481 atataacgac gagatatcat taaagacaaa tgcatag
//
LOCUS       DAZFYC010000094        18376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFYC010000094 DAZFYC010000000
VERSION     DAZFYC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.094"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..217)
                     /locus_tag="ACHKW5_003616"
     CDS             complement(65..217)
                     /locus_tag="ACHKW5_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7512940.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(482..1216)
                     /gene="cysH"
                     /locus_tag="ACHKW5_003617"
     CDS             complement(482..1216)
                     /gene="cysH"
                     /locus_tag="ACHKW5_003617"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7512941.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1290..3002)
                     /gene="cysI"
                     /locus_tag="ACHKW5_003618"
     CDS             complement(1290..3002)
                     /gene="cysI"
                     /locus_tag="ACHKW5_003618"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO7512942.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3002..4801)
                     /gene="cysJ"
                     /locus_tag="ACHKW5_003619"
     CDS             complement(3002..4801)
                     /gene="cysJ"
                     /locus_tag="ACHKW5_003619"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO7512943.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLVELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5120..5482
                     /gene="queD"
                     /locus_tag="ACHKW5_003620"
     CDS             5120..5482
                     /gene="queD"
                     /locus_tag="ACHKW5_003620"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO7512944.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5560..6831
                     /gene="ygcN"
                     /locus_tag="ACHKW5_003621"
     CDS             5560..6831
                     /gene="ygcN"
                     /locus_tag="ACHKW5_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7512945.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6822..7082
                     /gene="ygcO"
                     /locus_tag="ACHKW5_003622"
     CDS             6822..7082
                     /gene="ygcO"
                     /locus_tag="ACHKW5_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7512946.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7099..7674
                     /gene="ygcP"
                     /locus_tag="ACHKW5_003623"
     CDS             7099..7674
                     /gene="ygcP"
                     /locus_tag="ACHKW5_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO7512947.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7822..8682)
                     /gene="ygcQ"
                     /locus_tag="ACHKW5_003624"
     CDS             complement(7822..8682)
                     /gene="ygcQ"
                     /locus_tag="ACHKW5_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7512948.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8679..9458)
                     /gene="ygcR"
                     /locus_tag="ACHKW5_003625"
     CDS             complement(8679..9458)
                     /gene="ygcR"
                     /locus_tag="ACHKW5_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO7512949.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9436..10845)
                     /gene="ygcS"
                     /locus_tag="ACHKW5_003626"
     CDS             complement(9436..10845)
                     /gene="ygcS"
                     /locus_tag="ACHKW5_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7512950.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGISLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10867..12321)
                     /gene="ygcU"
                     /locus_tag="ACHKW5_003627"
     CDS             complement(10867..12321)
                     /gene="ygcU"
                     /locus_tag="ACHKW5_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO7512951.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12391..13176)
                     /gene="ygcW"
                     /locus_tag="ACHKW5_003628"
     CDS             complement(12391..13176)
                     /gene="ygcW"
                     /locus_tag="ACHKW5_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7512952.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13495..14772
                     /gene="yqcE"
                     /locus_tag="ACHKW5_003629"
     CDS             13495..14772
                     /gene="yqcE"
                     /locus_tag="ACHKW5_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO7512953.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14799..16277
                     /gene="ygcE"
                     /locus_tag="ACHKW5_003630"
     CDS             14799..16277
                     /gene="ygcE"
                     /locus_tag="ACHKW5_003630"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO7512954.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     repeat_region   16977..17249
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17038..17066
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
     gene            complement(17589..18260)
                     /gene="queE"
                     /locus_tag="ACHKW5_003631"
     CDS             complement(17589..18260)
                     /gene="queE"
                     /locus_tag="ACHKW5_003631"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO7512955.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT         4478 a         4770 c         4540 g         4588 t
ORIGIN      
        1 aaaaagggtg caggagacca aaccatcaca attcagtacg cggggtttta cccccgcgtg
       61 gtggctactt cttcgattcg taagcgagaa ctgccttaaa ctccatacca cgttctctga
      121 tactcaactc acacaacgag tcacctacca tcagcgtgaa tcccagtact gtacaacaca
      181 gtacgatgat tgccacaagg gcatattttg tcagcatgtc ttgcctccct gcaaagaaga
      241 gactaacatt cgaattgttt gggttcgaac gtaagcctca ggttgataga aatatcgcct
      301 ggggcttttg tccatctgga acctcgcgaa tgcttaacgc cagacagcct caagcacccg
      361 acgccattct atacctgata attcttccag cggcgttcac gtttaaaatc aggaatactg
      421 ctcgcaaaat aaaaacgccc ccatcatttc tgacagaggc gtttaattta tccggcaata
      481 tttacccttc gtgcaatcca cattcccttt ttaagccaaa gaaacgtgtt tcttcttccg
      541 acatgccagg ttcccattta cgggttgtat gggtatcgcc caccgataaa tatccttcgt
      601 cccataatgg gtgatatttc aggccatgtt tttgcagata ctgatatatc gttcggttat
      661 cccagtcgat aatcggcagc actttaaata cgccacgctg aattgccagc accggtaaat
      721 tggcgcggct gccggattgt tcgcggcgca ggccagcaaa ccacgtttgc gcattcagtt
      781 ctttcagcgc ccggttcatc ggttcgactt tgttgatttc attgtacttt tcaatgcctt
      841 caacgccctg ttcccacagt ttgccgtagc gtgcttcctg ccaggcggcg ctttcggtag
      901 cgcggtacac tttcaggttg agcttgagtt tgtccgttaa ctcgtcaata aagcggtagg
      961 tttccgggaa cagatagccg gtgtcggtga ggatcaccgg aatatccggg tgaatctgat
     1021 tcaccagatg caggctcacc gccgcctgaa tgccaaagct ggaagaaagt acatattcac
     1081 cgggcagatt atccagcgcc caggcaacgc ggccttcagc gtccagtttt tccagttcgg
     1141 cgttagtttc tgccagcgcc agaatgcgat ctaccttcgg cagttcgttc agggcgttta
     1201 gatcgagttt ggacatagat tcctcactgt ttgccttgcc tgatgcgacg ctcacgcgtc
     1261 ttatcaggcc tacaagaccg ggctgatggt taatcccaca aatcgcgcgc cggatcgagc
     1321 accgggcgaa tgatgcccgc acgcaccgta aagtcgccga agccttcacc cgcttcgcgc
     1381 tctttcgccc agcgccctat cagttcatca agcgacgcca ggatttccgg ctcggtgatg
     1441 ttttctttat acatccgtgg gatacgtgtc ccaatgcggt tgccgccaag atgcagattg
     1501 tagcgacccg gtgctttacc caccaggccc acttccgcca gcatcgcgcg accacaaccg
     1561 ttcgggcagc ctgttacacg catcacgata tgctcatcgc tgacaccatg tttcgccatt
     1621 aaattatcga tgttgtcgat aaaagacggc aggaaacgct ctgcttccgc catcgccagc
     1681 gggcaagtcg ggaatgacac gcaggccatc gagttttcac gctgcggcgt gacggcattc
     1741 attaaaccgc tctctttggc gatcttctcg atcttcgctt tctcgctttc cggtacaccg
     1801 gcgatgatca gattctggtt cgccgtaatg cggaaatcgc ctttgtggat cttcgcgatc
     1861 tccagcaggc cggttttcag cggacgcccc ggataatcaa ggatgcgacc attttcgata
     1921 aacagcgtca ggtgccagtt atcatcaatg cccttaaccc agccaatacg atcgcctcgt
     1981 ccggtgaact catatggacg gatcggttca aatttgatcc ccgcgcgacg ctccacttcc
     2041 gctttaaacg tctcaacccc cacgcgctcc agcgtgtatt tggttttggc atttttacga
     2101 tcggttcggt taccccagtc acgctgagtt gtcacgacgg cttccgccac cgccagcgta
     2161 tgctccagcg gcagatagcc aaactcgctc gccgtgcggg cgtaggtttt cttgttgccg
     2221 tgttcgatag aaagcccacc gcccaccagc aggttgaagc ctaccagctt gccgttttcg
     2281 gcgattgcca cgaagttcat gtcgttggca tgcagatcga tatcgttctg cggcgggatc
     2341 actaccgtgg ttttgaactt acgcggcagg taggtctgac caaggatcgg ttcttcatca
     2401 gtagtggcga ctttttcctg gtcgagccag atctccgcat acgcgcgggt acgaggcaac
     2461 agatgctcag aaatcttctt cgcccactcg tacgcttccg cgtgcagctg cgactcgtaa
     2521 gggttcgagg tgcagagtac gttacggttc atgtcgttag ctgtcgccag cgcatcaaga
     2581 ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct ttttcagaat gccgtggaac
     2641 tgaaacgtct ggcggttggt caggcgaatg ctgccataga tggtgttttc accggcaaat
     2701 ttgtcgatcg cctgccactg tttagtggta atcaccccac ccggcagacg acagcgaagc
     2761 agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt cggcgcggat gtcgcggtca
     2821 tcctgctgat acatgccgtg gaagcggatc agcaggaagt tatcgccctt aaagccgccg
     2881 gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca ggtagttgct ttcaagcttc
     2941 atgcgctcgg cgtctgtcag ttttccttcg accactaaag gccctggatg tttttcgctc
     3001 attagtagac atctcgctga taacggcgct ctacgcgcag ctcacttaaa aattcatccg
     3061 ccgcttcggt gtccatgcca ccaaattcgg caatcacttc cagaagtgcc tgctcaacgt
     3121 ctttcgccat gcgattagcg tcgccgcaga cataaatgtg ggcaccatca ttgatccagc
     3181 gccacagctc cgcgccctgt tcgcgcagtt tgtcttgtac gtaaactttt tctttttggt
     3241 cgcgcgacca ggcaagatcg atacgtgtca gcacgccatc tttgacgtag cgctgccact
     3301 ccacctggta caggaagtct tccgtaaagt gcggattacc aaagaacagc cagtttttac
     3361 ctggcgcttc gtcggcggcg cgttgctgca taaaggcgcg gaacggcgca ataccggtgc
     3421 ctgggccaat cataatcacc ggggtttccg ggttagcggg caggcgaaag ttatcgttat
     3481 gttcgataaa tacgcggact tcgccctctt cttccacgcg atccgcgagg aagctggagg
     3541 caccaccggc acgggctcgg ccttccacgt cgtaacgcac cacaccaacg gtgacgtgta
     3601 cttcgttctc gacttccgcc tgcgaggagg cgatggaata caggcgcggg gtcagcgggc
     3661 gcagcagatt aatcagcgct tcggcatcca gttgcgccgg agagaaacgc accatgtcga
     3721 caatcggcgt cgtcgcggcg taatgctgta acttcgcttt atcgcccacc agcggcagca
     3781 gtgtttcact gcgggtaagc gtggcgtaat tctcaacaat gttggcggtg ttgacggtca
     3841 gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt tttgccctcg acggtgacag
     3901 gttcatcgcc tttcagccac agcagttcga caagttcttt caccagtgcc ggatcgttct
     3961 gataccagac gcccagcgcg tcacccggct ggtaacgcag gcccgagtca cctaagtcaa
     4021 tttcgatatg gcgaacgtct ttttcagagt tacgtccggt aattttctgg ttaaccgaaa
     4081 ggctcgccac cagcggagcg tctttgctgt acgggctgga gtggatttca tttaccgcgc
     4141 cagtagcgac ggattgcgaa ggtgccgcga caggcgcacg cgatttaagc gcatcaacca
     4201 cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac atcggcatcg acacggtcga
     4261 gcaggcgttc accgcccagt tccaccagct tgctgtcaaa atctttcccg gactggcaga
     4321 aaaattcata agagctatcg ccgaggctaa acacggcaaa tgcggtgttt tccagcttcg
     4381 gcgctttttt ggagaacagg aacttatgca gcgcgacggc ttcttccggc ggttcccctt
     4441 ctccttgcgt tgacgtcacc acgataagca gtttttcgct ggcgatttgt ttgaatttat
     4501 agtcgcccgc gttcaccagc ttaacgttca gttttgctgc taataaatca tcgcgtaatg
     4561 cttcagcaac ccggcgcgca ttgccggttt gcgaggcgga gataatagtt atacccggca
     4621 tttctgcggc tggcgctggc gtcgctgcaa gcgcagcagg ctgctgattg agtacgcccc
     4681 agaaatagcc agaaacccag gcaagctggg tgggagttaa atcggtcgtg gccgcctgaa
     4741 ggcgtgccag ttgctccggg ttcaacggaa gcaacgcgga aggtgggacc tgtgtcgtca
     4801 tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc ctgtcagcgc ccataaaaca
     4861 gaagagaagg taaggttaac ggggtgaacg gtgtggatta aagacgggat agcgataact
     4921 aataaccaaa tcgactaacc tgttttagca atagtcttta acaacaaaat agattaacca
     4981 acctaatgaa aaacaaatga atttagccaa tcattaagat aaatcagcga ttttgcgcaa
     5041 caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg tactctacgg cggttttatc
     5101 gtcctgtaga gaaattatga tgtccaccac gttatttaaa gatttcacct tcgaagccgc
     5161 tcaccgctta ccacacgtcc cggaagggca taaatgtggt cgcctgcacg ggcattcctt
     5221 tatggtgcga ctggaaatta ccggggaagt cgatccgcat acgggctgga ttatcgattt
     5281 cgctgaacta aaagcggcgt ttaagccaac ttatgagcgc ctcgatcacc attatctcaa
     5341 tgatattcca ggtctggaaa acccaaccag cgaagtttta gcaaaatgga tttgggatca
     5401 ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta aaagaaacct gcaccgctgg
     5461 ttgtatctat cgcggcgaat gataagagtg tgtcggcggt caatttccct taagtaacgc
     5521 tatgttaggg tgttgtgttc tggatatctg gggcatgaca tggaagacga ctgcgacatt
     5581 attattattg gtgccggtat tgcaggcacc gcttgcgcgt tacgctgcgc gcgagcgggt
     5641 ttatccgttt tgttactgga acgcgctgaa atccccggca gcaaaaatct ttccggcggg
     5701 cggttatata cccatgcact cgcggaactc ctcccgcaat ttcatctgac cgcgcctctt
     5761 gaacgacgca tcactcacga aagcctttcc ctgttaacgc ccgatggcgc aacgacgttt
     5821 tccagcttac agcccggcgg tgaatcctgg agtgtattac gtgcacggtt cgatccgtgg
     5881 ctggttgccg aagccgaaga agaaggtgtc gaatgcatcc ccggagcgac ggtggatgca
     5941 ctgtatgaag aaaacggcag ggtgtgtggt gtcatttgtg gtgacgatat tctccgtgcc
     6001 cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg ctgagcgtca cgggttagtg
     6061 actcgtcctg ctggtgaagc gatggcgttg gggatcaaag aagtgctgtc gctggaacca
     6121 tccgctattg aagaacgttt tcatctggag aataacgaag gcgcagcgtt gctgttcagc
     6181 ggcaggatct gtgatgactt acccggcggc gcatttcttt atactaatca acaaacgctc
     6241 tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa gtcgtgttcc ggcaagcgag
     6301 ctgctgactc gctttaaagc gcatccggca gtgcgcccgc ttatcaaaaa cacggaatca
     6361 ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc acagtatgcc ggtgcaatac
     6421 gccggtaacg gctggctgct ggtgggcgat gcgttgcgca gttgcgtcaa taccggaatt
     6481 tccgtgcgcg gcatggatat ggcgctgact ggcgcgcagg cggcggcaca aacgctgata
     6541 agcgcctgcc agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtagag
     6601 cgcagcctgc tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc
     6661 cctggctggt atcgggcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat
     6721 cagggtgata aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcat
     6781 ggcctgtggc atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccataatc
     6841 tctggcgcgt tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg
     6901 tacaacggtt gattaacgcc tgtccggcag gtcttttttc gctcacaccg gaaggtgact
     6961 tacgcgttga ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat
     7021 ccacactaca acagtggcgc tatccgcctt ccggattcgg catcacctac cgctttggat
     7081 aaataataag gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct
     7141 gccgttaaag ataatgccag cctgcaactg gcaatcgact ctgaatgcca gtttatttcc
     7201 gtgttgtacg gcaatatctg caccatcagt aatatcgtta aaaagattaa gaacgccggg
     7261 aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt
     7321 cagtttttga aactggtgac cgaggcggac ggcattatca gtaccaaagc ctcaatgttg
     7381 aaagcggcaa gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcaatt
     7441 tcgtttcaca acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg
     7501 ccaggctgta tgcccaaagt gctgggctgg gtgacagaga aaatccgcca accgctgatt
     7561 gccggtgggc tggtgtgcga tgaagaagat gcgcgtaatg cgattaacgc gggtgtcgtg
     7621 gcgctttcca ccacgaatac cggggtctgg acgttagcga aaaaattact ttgacgggat
     7681 aatcgtaacc aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc
     7741 gcctgatgcg acgcttgacg cgtcttatca ggcctacaag tccccaggcc agaaccgtag
     7801 gcaggatcat ccggcaacac gttattggca atctgcgtga atgtttgtca ccagtgcctc
     7861 cagcaccact ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct
     7921 ggcgtcatga ttaatcgcca ccacaaactt gctattgcgt acgcccgcca ttaacgctgc
     7981 tgcgccagaa gcgccaacaa caatacacac ctcaggtgcc agcagatgtc cggaaatacc
     8041 aatcactttt tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc
     8101 ggctcccagc ttttctgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc
     8161 caccaccagc acccgtctgg cctcagctaa tgggtcgcga gtaacatttt tcagatcctc
     8221 tgtgctaacc agccagtcgg gaagcgcacc cgggacaatg ttcagttgtt gcattccaga
     8281 gggcaacgtg gcgtttttcg ctgctcccgc ctgacgcgcc agggaaaggc atagcgggcg
     8341 tttttctgtt tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac
     8401 ggttggaata tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag
     8461 ccgggtcgac agttcatcgc caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt
     8521 tcgctgccac tgttccacca acgcatccag cacctgttcc gccactacag gctgaggttc
     8581 aatctgccag tgcgccaggg tacaaccgct gaaatcctgc gccgccagcc agcttgctat
     8641 tgcggcattc tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt
     8701 aaatagtcct gccatagctt ttgagctttt tctgctaccg tttgcccatc aatcagcgtc
     8761 gctccgcgtc gttgctctgc tcttgccagt tgcagacact gcatcgctgg catttcggca
     8821 gcaacagttt tacggataat ttctgctttc cccgcagcca ttcgctggcg cattcccggt
     8881 actggcaagg ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg
     8941 caacaacgca acccatgttc agtacgctgt tcgagggtga taaacagcgc gtcgagagtg
     9001 aaacgctcca cctgggtgaa gcagggccag cccagcattt ccgccagcaa aaatggcgtt
     9061 tgcccattct gcccttcgct gctttggcag ccggtaatta tcagatccag cggattctga
     9121 tgctgccatt cggcaatatg gcgagcgaca aattccggcg caaagcgcag atctgccgcc
     9181 gtctccagca atacagcttc ctcaaaccca agagccatga gatagcgcag ccagtgcaat
     9241 gcccgttcat cccccatgct caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc
     9301 gccaacagca gcgcggcagc ggcctgttca tcagcaccga gtaaacttcg cagtagcgaa
     9361 atatccggtc cgcttttacc ctgagccgcc gcctgccact ctttttccgc cagcattccg
     9421 gcatccggtt cggctttaaa cgctaacaga atgttcattc gcacctccta catttcccgc
     9481 cgccaccagc gggagggctt tagtttctgg tgcccatagc caggtcacaa caaaaccaac
     9541 caacaacact gtcgccagga gcaataaggt gacttgcatt ccccactgcg ccagcaccca
     9601 cggcagcagg ccagtactta ccgccgcgcc aagtcgactc atggcggtgg caaaaccgac
     9661 gcccagcgag cgaatgtcag taggaaaact ttccgcaggc aaaatgccca ccagattact
     9721 gactgccgaa atggtggtgc tgaagagaac aaaaagcagc agcgttaatg aactgccgga
     9781 aggcaaacag gccatgacta ccagcgttgc cgccagcagc aaaaaacttc ccagcaaaaa
     9841 tttgcgatgt gccagcaggt gcgtcagaac taatcccaac agcgcgccca caattaacaa
     9901 cgcattaagc atcaggctgg cagtcagcgc atcttccaga ccaatagtct gggcgatagt
     9961 tggcagccag gtataaatca caaaccatgg gattacgagg cagacaaaga agacgctgtt
    10021 aaacgccgtg cgccgccagt aacgcgaaga gaacaaggtt ttgatgtgtt tatgggtcgc
    10081 cgttaccact tcatcgccca gtaaaacatg gggaccaaaa tagcgatgca cgatagcgtg
    10141 agcttctgca aaacgcccct ggcgcagtag ccagcgtggt gattctggcg ttccccagcg
    10201 taataacgta atcaacaacg cgggcagagc tgccgatgcc agtagccagc gccaggcctc
    10261 cgggttttcg gaaataaagt gatgtccggc aatacttgcc agcacatagc ctacggtcca
    10321 caccacgctg aatgcgccca gcaaaatacc gcgatggcgg cgcggggaaa attcagccag
    10381 caaggtgtga cctactgaat aatcgcctcc cagactaatg ccaatcaaaa tgcgcagtcc
    10441 aataagatgc tctggcgtgg tggcaaaaaa ttgtaaaaac gaagcaagcg taatcagcaa
    10501 aaagctgaag gtgaagattt tttgccgacc aatatggtcg gagatccacc caagaaccag
    10561 gctgccaagg aacaaaccaa ggagcgccga gccgccgatc attcccgcca taaacggcgt
    10621 cagttgcatg gcgggcgtaa gctgaataat ggcgtaacca atgacgccga gaacataacc
    10681 gtcggtcagg tgtgcgccga aagtgagcgc agcaatgcgg cagtgaaaac ggttaagcgg
    10741 taaatcatcc attcgcaccg gtgaagtgtt catctttacc ccttcattgt ccggtaataa
    10801 gaacggcttc tcgcctgaga agccgaaaag gcaacgcccc gtcatcgcgg aacgctgcct
    10861 gacacattat ttttcaatcg gatagatagt acccgtattc ataatgccat taggatcgaa
    10921 ctgctttttc agcccttcca gcaacgccca cgcgctgccg tgttccagtt tgctccagtg
    10981 aacgcgatgt ttaccgatac cgtggtggtg caccatcgaa ccgccgaggc gaatggtttc
    11041 ttcacagatg atcttgttga gcggattgtg gtacttgtcg atttcctctt ccggcttaca
    11101 gtcaacgacg ttgtaatcgt agacgaagta catgttggtg ccgttctgat agctatgaga
    11161 ggaatgaccg cccagcatgg tgatgtcgtc ggcgtgcggg aactcagtac gaatacggtt
    11221 aataacgctt tcgtagattt cgtggatgca gctccagcag ccggacactt cagtggtaaa
    11281 gcccatgttg ccggttttga ggatctgcac acgttcggca gccactttat ccggtcccca
    11341 gttcaggttg ttaaaccagg tttcgatcag cttgctgtcc acgcgctggc attgcgggta
    11401 gcgggcaacg atttccgcaa tcccttcgcc cgtcgccttc gcaatgcgag ggttaccttc
    11461 agccataaag atcagcacgc attttccgtc tgcaaaatgg gtgaagtgtt gggtgccatc
    11521 ttcagcgtca tacaaacgag cgatcgacgg acgataccct tccaccatga cttcacgcag
    11581 gatgttgaag ccggttttca tgtcttccag gatatagccg tagaagaggt tgttttccgg
    11641 ggtgaattta aagattttca ctgttacttc agtgatatag caaagtgcac cttcgttacc
    11701 gatgatgatg tggcgaatgt ccgggccagc cgcgcggcgt ggcacgtttt taatgcgtgt
    11761 gacggtgcca tcagccaata ctgcttccag accaacgacc atatcttcga ttgcgccgta
    11821 gagtgtggag aactgcccga tactgcgggt tgctaccagg ccgcccatct gagccagcgg
    11881 ctttgactgc ggagaatgcc ccgtggtgta acctttttca cgcaacgcat tttccagcac
    11941 ctccagcgga acgccacatt gcgccgtcgc ctgcatattc tcaatatcaa tattaatgat
    12001 ttgattcatg gcggagccgt cgagcaccac cgagttttcc acaacggttt ccagcccacc
    12061 ttcagtggcg gaagcgccgg tacgcggcac accgttaatt ttgtgcgcat tcataaaatt
    12121 cagcacacgg gagacttgtt ctgtggaacc gagttttacg accgctgccg gaatcggcaa
    12181 agtataaata ccatgaatat ccggaaattt acgaaaacgg tcaatactgt tcttctttaa
    12241 tactgtttca tcggtaatta cgcgatctgc accaacaatt tcctttagct ggtcgacaat
    12301 cgctgcgcga gataaagaca tagtaattcc ttcctgataa taaaaatgga ataatcatta
    12361 ataagagaaa acagaaacca ccgttatgga ttagcgcact aaataaccac catcgaccac
    12421 taataaatgc ccattgacat aatttgaagc cggacttgcg aggaatacag ctgcgcccat
    12481 taaatcctga gtatcgcccc aacggtttgc cggaatatga tcaagaacgc gctgattggt
    12541 ttctggatta ctgcgtgtcg ccagcgtaat atctgttgca taatagccag gggcgatacc
    12601 atttacctga atattatatt gacctagctc atcacaataa gctttggtga acccggcaag
    12661 agcatgttta gtggcagaat atgcaggtga ccattgtcca cctaagtaag agaacaatga
    12721 acagatatta ataattttgc cgcttttctg cgggatcata atttttgcag cttcatagct
    12781 taactcgaat gcggcggtca ggttcacatc aatcatcgga tcccagtcgg cacgaccgaa
    12841 gtccagcacc ttattcagct tacaaatacc ggcattgttt accagaatat caactgtacc
    12901 gaaacgctca cagcaagcag cgataatctt ctgcggcgcg ccttctgcgg tgatatccac
    12961 ctgcatgaag tccacctcaa caccctgttt ttcaatcatt tcctttgttt cgccgttatc
    13021 tttgacgaaa ctaggaataa agatatttgc gccagctttg gccaacgcca tggcaaatgc
    13081 ctggcctaaa ccgctattcc caccggtaac aattgcggtt ttacctttca gtgagaaaaa
    13141 atccattgag aacgcattga gagattcgat tgacatagtt agctccaatt tccgtaaggc
    13201 aaaaaaaaag aaaggtaact tcccccgcga gagggaagtt acctttctca ttatctctgg
    13261 taactggaga taatttttag cataacggca taactgctaa tgtgatcgta atcacagtgt
    13321 gatattcgta ttgtcaaata gttttaaaaa gcagaagcgt gattactctc acacaacacc
    13381 tctgctttcc atgttatttc ttattgcaat tactagagac ctgagaaaag taatccacct
    13441 gaaagggcgg cttacttttc tctttttttt gtctattaca aatgggaata cgatatgcaa
    13501 cacaactcat atcgccgttg gataaccctc gcgataatta gttttagcgg cggcgttagt
    13561 ttcgacctgg cttatttacg ttatatttat caaattccca tggcgaaatt tatgggattc
    13621 agcaataccg agataggttt aataatgagt acctttggta ttgcggctat tattctttat
    13681 gcccccagcg gcgttattgc cgataaattt tcacaccgca aaatgattac ttccgcgatg
    13741 atcattaccg gattactggg tctgttaatg gcaacgtatc caccgctgtg ggtaatgctc
    13801 tgtattcagg tcgcctttgc gataacgacg attttaatgc tgtggtcggt gtcgattaaa
    13861 gccgcctcgt tgctgggcga tcatagtgag caagggaaaa ttatgggctg gatggaaggg
    13921 ctgcgcggcg tcggtgtaat gtcgctggcg gtgtttacca tgtgggtctt ttctcgcttt
    13981 gcaccggatg acagcgccag cctgaaaaca gtcattatca tctacagtgt ggtttacatc
    14041 ttgttgggga ttctgtgctg gttttttgtt agcgataaca acaacctgcg cagtgccaat
    14101 aacgaagaaa aacagtcatt ccagcttagc gacatccttg ccgttttgcg tatcagcacc
    14161 acctggtatt gcagcatggt gatttttggt gtcttcacaa tctacgccat tctgagttac
    14221 tccaccaact atctgaccga aatgtacggc atgtcgctgg tggcagcgag ctacatgggg
    14281 attgtgatca acaaaatctt ccgcgcgctg tgcggcccac ttggcggcat tatcaccacc
    14341 tacagtaaag tgaaatcccc tacccgcgtg atccaaatcc tttccgtact cggcctgctg
    14401 gcgttaactg ccctgctcgt cacgaactct aacccgcaat cggtcgcgat ggggattggc
    14461 ctgattttac tgctgggatt cacctgttac gcctcacgcg ggctgtactg ggcctgccct
    14521 ggcgaagcga gaacaccgtc ttacattatg ggcaccacgg taggtatttg ttcggtgatt
    14581 ggattcctgc cggatgtctt cgtttacccg attatcggcc actggcaaga caccctgccc
    14641 gcagcagaag cctaccgcaa tatgtggctg atgggaatgg cggcgcttgc catggtgatt
    14701 gtctttacct ttttgctgtt ccaaaaaatt cgtactgctg atagcgcccc cgcaatggct
    14761 agcagcaagt aagccaggaa aattacagga gaaaaatcat gtcgaaaaaa tacatcatag
    14821 ggattgatgg cggaagtcag agcacaaaag tggtgatgta cgatctggaa ggtaacgtgg
    14881 tttgcgaagg caaaggctta ttacagccga tgcacacgcc agatgccgat actgcagaac
    14941 atcctgacga cgatttatgg gcatcattat gttttgccgg tcacgatttg atgagtcagt
    15001 ttgctgggaa taaagaagat attgtcggta ttggtctggg atccatccgt tgctgtcgtg
    15061 cgttattgaa agccgatggc acgcctgctg cgccgttgat tagctggcag gatgcacgcg
    15121 ttacacgccc ttacgaacac actaatcctg acgtggcata tgtcacctct ttttcgggtt
    15181 atctgacgca tcgcttaacc ggcgagttta aagacaatat cgccaactat tttggtcagt
    15241 ggccggtgga ttataagagc tggacatgga gcgaagatgc tgcggtaatg gataagttta
    15301 atatcccccg tcatatgctg tttgatgtgc aaatgcctgg caccgtcctc ggacatatca
    15361 caccacaagc cgcactggcg acacatttcc cggcaggact gccggttgtt tgtaccacca
    15421 gtgataaacc ggtagaagct ctgggggccg gattactgga tgatgaaact gcggtaattt
    15481 ctcttggcac ttacatcgca ttgatgatga acggcaaagc actgccgaaa gatccggtgg
    15541 cgtactggcc gattatgtct tctattccgc aaacattgct gtatgaaggt tacggtattc
    15601 gcaaaggtat gtggacggtg agctggctgc gtgacatgtt aggcgagtcg ttaattcagg
    15661 atgccagggc gcaggatctt tcaccggaag atttactcaa caagaaagcc tctagtgtgc
    15721 cacctggctg taatgggctg atgacggtgc tggactggct gaccaatccg tgggaaccgt
    15781 acaaacgcgg gattatgatc ggctttgatt ccagcatgga ttacgcatgg atatatcgtt
    15841 cgatattgga aagcgtggcg ctaacgctga agaacaatta cgacaatatg tgtaatgaaa
    15901 tgaatcactt tgcgaagcat gtgatcatta ctggcggcgg ttcgaacagc gatctgttta
    15961 tgcaaatttt tgccgacgtg ttcaaccttc cggcacgccg taacgccatt aacggttgtg
    16021 caagtctggg agcagcgatt aatacagcgg taggtctggg gctatacccg gattacgcaa
    16081 cggctgttga taagatggtt cgcgtgaaag atatctttat accgattgag agcaatgcca
    16141 aacgctacga cgcgatgaat aaaggcattt tcaaagacct aaccaaacat actgatgtga
    16201 tcctgaaaaa atcgtatgaa gtgatgcatg gggaattggg gaatgtggat tcgatccaga
    16261 gctggtcgaa tgcgtaagtg aggaaggccg ggcgggaaac tgcccggcct gaacatacct
    16321 gaatggttat ccccgctgac gcggggaaca taagttcgac aacttaaaaa atgataacaa
    16381 gtccggttta tccccgctga cgcggggaac ataagttcga caacttaaaa aatgataaca
    16441 agtccggttt atccccgctg atgcagggaa cataataaaa actttaacta gttcggttta
    16501 tccctgtttt tacaaggaat attaactaca ctcgatgtct ttagaataaa agacaatacg
    16561 aatcgcactt tatcaatttt cacttaaagt atgaactgga tatctttatt cataagaact
    16621 attcatcaac agcgtataga ggcggttatg taaaaccact cattagcctc aaaacaaaga
    16681 caaacttcca gatttattac ttaaccaatg aataacaagc ttaaaaaaca tactgttatt
    16741 ctaaagaaaa aattaacagg cattaattca atattaaaaa aataatagat taaaatttct
    16801 taacgattta agaatcatac aaataacact ttgattaaat ttaatttttt gtatcgataa
    16861 ttgtgaacct ctctggcatg gagaactatt ttgaacatga ggtgttacgt ggatatgttg
    16921 cttattacaa gtactgctaa tataaaaact tgagaaagag ataacgggtt atatggtggt
    16981 ttatccccgc tggcgcgggg aactcgcctg acattgcaga cgttacaaat tgagaggcgg
    17041 tttatccccg ctggcgcggg gaactctaat cacgttttag cgcgccctcg tccggtttcg
    17101 gtttatcccc gctggcgcgg ggaactcatc acgataacgc tgctgtgatt cgtccccgtc
    17161 ggtttatccc cgctggcgcg gggaactcaa gacgacgtga tccgcaaagt cgaaggcacg
    17221 cggtttatcc ccgctggcgc ggggaactct gtaaacataa cctattatta attaatgatt
    17281 ttttaagcca gtcacaatct accaacttta tagtatcaca caaacaacac atccattatg
    17341 ttaaagagca cttaatccat tgattaaaaa ggtaaatatt taaaataact ctatacaaac
    17401 taaaatctac caaactttac cgcaataatt ttcactccag cgaaaaatta atgccacaga
    17461 atttgtagag aatgctaatg attagccctg gatgggtaaa atggaggagt tttcagaggc
    17521 gacaaacaat ataaatgagt agaagtcttt acaggttcca cccatttata acgcttataa
    17581 atgtttaatc aggcaatatt tagatattta tgtgtttgca tcgacaaacg ccaattacgc
    17641 gcaatgcagg tttcaatgca caaacgtgtg gcatcatcct tttggctaat cggctgcagt
    17701 gcaatgactc gcggtttatc atcggtcagc gttgccagta gttcatccag tgcttcaata
    17761 tcacgtacgc gccccactgg atgcttgatt tcgttggctc gctccagtgc ctgtgacaac
    17821 acgtcatagc cgccgcgcat gttcagcttt ggcgatacgg taacccaggt attcggtgtg
    17881 cagcgtactt catgagttcc actggtttcg atctggcagc taaaaccgtt cttttcgagc
    17941 aggtcagtca gtggcagcaa atcatgaatg caaggctcac cacccgtaat caccacatgc
    18001 cgcgcggtat atccctgccg accaatgaca gccagcaaat cttcactgct cgccgccccc
    18061 cacttatcac tctctttggt cttcgccaga atgctgaaaa gggagacttc ccgatcctca
    18121 agcttttccc aggtgtgttt ggtgtcgcac caggcacagc caaccgggca tccctgtaaa
    18181 cgaataaaaa tggcggggac gccggtaaag taaccctcac cttgcagggt ctggaacatc
    18241 tcgttaatcg ggtactgcat agcattctct gtgaagtgga taattgttaa ttattgcaga
    18301 tcctgccgca acaatcatgt cttattaaca ttctgttaca ggcagattta aagaaaaatg
    18361 cgaaacatat cgttaa
//
LOCUS       DAZFYC010000095        18368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFYC010000095 DAZFYC010000000
VERSION     DAZFYC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.095"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..1213
                     /gene="arnB"
                     /locus_tag="ACHKW5_003632"
     CDS             74..1213
                     /gene="arnB"
                     /locus_tag="ACHKW5_003632"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO7512956.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1217..2185
                     /gene="arnC"
                     /locus_tag="ACHKW5_003633"
     CDS             1217..2185
                     /gene="arnC"
                     /locus_tag="ACHKW5_003633"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO7512957.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2185..4167
                     /gene="arnA"
                     /locus_tag="ACHKW5_003634"
     CDS             2185..4167
                     /gene="arnA"
                     /locus_tag="ACHKW5_003634"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO7512958.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4164..5054
                     /gene="arnD"
                     /locus_tag="ACHKW5_003635"
     CDS             4164..5054
                     /gene="arnD"
                     /locus_tag="ACHKW5_003635"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO7512959.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5054..6706
                     /gene="arnT"
                     /locus_tag="ACHKW5_003636"
     CDS             5054..6706
                     /gene="arnT"
                     /locus_tag="ACHKW5_003636"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO7512960.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPQLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6703..7038
                     /gene="arnE"
                     /locus_tag="ACHKW5_003637"
     CDS             6703..7038
                     /gene="arnE"
                     /locus_tag="ACHKW5_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO7512961.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7038..7424
                     /gene="arnF"
                     /locus_tag="ACHKW5_003638"
     CDS             7038..7424
                     /gene="arnF"
                     /locus_tag="ACHKW5_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO7512962.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7418..7684)
                     /gene="pmrD"
                     /locus_tag="ACHKW5_003639"
     CDS             complement(7418..7684)
                     /gene="pmrD"
                     /locus_tag="ACHKW5_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO7512963.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7794..9149)
                     /gene="menE"
                     /locus_tag="ACHKW5_003640"
     CDS             complement(7794..9149)
                     /gene="menE"
                     /locus_tag="ACHKW5_003640"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO7512964.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9146..10108)
                     /gene="menC"
                     /locus_tag="ACHKW5_003641"
     CDS             complement(9146..10108)
                     /gene="menC"
                     /locus_tag="ACHKW5_003641"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO7512965.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10108..10965)
                     /gene="menB"
                     /locus_tag="ACHKW5_003642"
     CDS             complement(10108..10965)
                     /gene="menB"
                     /locus_tag="ACHKW5_003642"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO7512966.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10980..11738)
                     /gene="menH"
                     /locus_tag="ACHKW5_003643"
     CDS             complement(10980..11738)
                     /gene="menH"
                     /locus_tag="ACHKW5_003643"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO7512967.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11735..13405)
                     /gene="menD"
                     /locus_tag="ACHKW5_003644"
     CDS             complement(11735..13405)
                     /gene="menD"
                     /locus_tag="ACHKW5_003644"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO7512968.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13494..14789)
                     /gene="menF"
                     /locus_tag="ACHKW5_003645"
     CDS             complement(13494..14789)
                     /gene="menF"
                     /locus_tag="ACHKW5_003645"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO7512969.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="ACHKW5_003646"
     CDS             complement(14868..15173)
                     /gene="elaB"
                     /locus_tag="ACHKW5_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO7512970.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15228..15689)
                     /locus_tag="ACHKW5_003647"
     CDS             complement(15228..15689)
                     /locus_tag="ACHKW5_003647"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7512971.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15754..16671
                     /gene="rbn"
                     /locus_tag="ACHKW5_003648"
     CDS             15754..16671
                     /gene="rbn"
                     /locus_tag="ACHKW5_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO7512972.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16859..18079
                     /gene="elaD"
                     /locus_tag="ACHKW5_003649"
     CDS             16859..18079
                     /gene="elaD"
                     /locus_tag="ACHKW5_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFO7512973.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLTRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            complement(18167..>18368)
                     /locus_tag="ACHKW5_003650"
     CDS             complement(18167..>18368)
                     /locus_tag="ACHKW5_003650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512974.1"
                     /translation="YSGFAILKRNSYKEKYNYVIYWQIELTGGRTLRVRKVSNNNPLH
                     QQTSNRVPRYAGNTRRLNWRMV"
BASE COUNT         4414 a         4885 c         4570 g         4499 t
ORIGIN      
        1 aatggcaaaa tatacacaac actctttata gcaaatataa gtggacaggt attcaatggc
       61 ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga ccagcaatgg gcgtggagga
      121 actcgctgca gttaaagagg ttctcgaatc cggttggctc acaaccggtc cgaagaatca
      181 ggcgcttgag caagcctttt gccagttgac gggaaatcag catgccatcg cggtcagttc
      241 agccaccgcc ggaatgcata tcacgctaat ggcgttggaa attggcaagg gcgatgaagt
      301 gattacgcct tccctgacct gggtttcaac cctcaatatg atttccttgt tgggtgcaac
      361 gccggtaatg gtggatgtcg accgcgatac gctgatggtc acgcctgaag cgatcgagtc
      421 agccataacg ccacgcacta aagccatcat tccggtgcat tatgccggtg cgccagcaga
      481 tattgacgcc attcgcgcca ttggcgaacg ttacggcatc gcagttatcg aagatgctgc
      541 ccatgccgtc ggtacgtatt acaaagggcg acatattggc gcaaaaggta ccgctatttt
      601 ttcatttcat gccattaaaa atattacctg tgcggaaggt ggcctgattg taactgataa
      661 tgaaaacctt gcccgccagc tacggatgct gaaatttcac ggtctgggtg tcgatgccta
      721 tgacagacaa acctggggcc gtgcaccgca ggctgaagtc ttaacaccgg gctataagta
      781 caatctgacc gatattaacg ccgcaattgc cctgacacag ttagcaaaat tagagcacct
      841 caacacccgt cggcgcgaaa ttgcccagca atatcagcaa gcactggcag ctctcccctt
      901 tcagccatta agccttcccg cctggccgca cgttcacgcc tggcatctgt ttattattcg
      961 tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg atggaagcgt taaaagaaag
     1021 aggcattggt accgggttac atttccgcgc cgctcacaca caaaaatatt atcgcgagcg
     1081 ttttcccacg ctgtcgttac cgaataccga atggaatagc gaacgcatct gttctttgcc
     1141 gctgttcccg gatatgacta ccgccgatgc cgaccgtgtc atcacagccc ttcagcaact
     1201 cgcaggacaa taagccatgt ttgaaatcca ccctgttaag aaagtctcgg tggttattcc
     1261 cgtttataac gagcaggaaa gcttaccgga attaatcagg cgcaccacca cagcctgtga
     1321 atcgttgggg aaagagtatg agatcctgct gattgatgac ggcagtagcg ataattccgc
     1381 gcatatgctg gtcgaagcct cacaagcgga gaacagccat attgtgtcta ttttgcttaa
     1441 ccgcaattac gggcaacatt cagcgattat ggcgggattc agtcacgtta ccggcgactt
     1501 aattattacc cttgatgccg atctccagaa tccgccagaa gaaatccccc gcctggtggc
     1561 aaaagccgat gaaggttacg acgtggtagg gactgtacgc cagaaccgcc aggacagctg
     1621 gtttcgtaaa acagcttcga agatgattaa ccggcttatt cagcgcacca ccggcaaagc
     1681 gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt catattgtcg atgcgatgtt
     1741 gcactgccat gaacgtagca cctttatccc gattctggcg aatatcttcg cccgccgtgc
     1801 cattgaaatt ccagtacatc attccgagcg tgagtttggt gaatccaaat acagttttat
     1861 gcgcctgatt aatttgatgt acgacctggt gacctgcctt accacaacgc cgctacgtat
     1921 gctgagtctg ctcggcagca ttattgcgat tggaggtttt agcattgcgg tgctgctggt
     1981 gattttacgc ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta tgctatttgc
     2041 cgtgctgttt acttttattg gcgctcagtt tatcggcatg ggattactcg gtgaatatat
     2101 cggcaggatc tacaccgatg tccgcgcccg cccccgctat tttgttcagc aagttatccg
     2161 tccatccagc aaggaaaatg aataatgaaa accgtcgttt ttgcctacca cgatatggga
     2221 tgcctcggta ttgaagctct gctggctgcc ggttacgaaa ttagcgccat ttttacccat
     2281 accgataatc ccggtgaaaa agccttttat ggttcggtgg ctcgtctggc agcggaaaga
     2341 ggcattccgg tttatgcgcc ggatgacgtt aatcatccgc tgtgggtgga acgcattgcc
     2401 caactgtcgc cagatgtgat tttctctttt tattatcgcc atcttatttg cgacgaaatt
     2461 ttgcagctcg ctcccgcagg tgcatttaat ctgcatggtt cgctgttacc aaaatatcgt
     2521 ggtcgcgcgc cgctgaactg ggtgctggtc aacggtgaaa cggaaactgg cgttacattg
     2581 caccgaatgg tgaaacgtgc cgatgccggg gccattgtgg cgcaactgcg cattgccatt
     2641 gcgccagacg atatcgctat tacgctgcat cataaattgt gccatgccgc gcgccagcta
     2701 ctggaacaga cattacccgc cattaaacac ggtaatattc tggaaatcgc ccagcgcgaa
     2761 aacgaagcca cctgttttgg tcgcagaacg ccggatgaca gtttccttga atggcataaa
     2821 ccggcatccg tactgcacaa catggtacgt gccgttgccg atccgtggcc gggtgccttc
     2881 agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc gtgttcatcc tcatgccagc
     2941 aaagcacagc cggggagcgt gatttctgtt gcgccactgc tgattgcctg tggcgatggc
     3001 gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg ctcgcaatta
     3061 gcgcagacgc tgggcctggt gcaaggttca cgcttgaata gccagcctgc ctgcaccgcc
     3121 cgacgccgta cccgggtact catcctcggg gtgaatggct ttattggcaa ccatctgaca
     3181 gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc tggatattgg cagcgatgcg
     3241 ataagccgtt ttctgaatca tccgcatttt cactttgttg aaggcgatat cagtattcat
     3301 tccgaatgga ttgagtatca tgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata
     3361 gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga ttttgaagag
     3421 aatctgcgca ttatccgcta ctgcgtgaag taccgtaagc gaatcatctt cccgtcaact
     3481 tcagaagttt atgggatgtg tagcgataaa tacttcgatg aggaccattc taatttaatc
     3541 gtcggcccgg tgaataaacc acgctggatt tattcggtat caaaacaatt acttgatcgg
     3601 gtgatctggg cctatggcga aaaagagggt ttacagttca ccctcttccg cccgtttaac
     3661 tggatgggac cgcgactgga taaccttaat gcggcacgaa tcggcagctc ccgcgctatt
     3721 acgcaactca ttctcaatct ggtagaaggt tcaccgatta agctgattga tggcggaaaa
     3781 caaaaacgct gctttactga tattcgcgat ggtatcgagg cgttataccg cattatcgaa
     3841 aatgcgggaa atcgctgcga cggtgaaatt atcaacattg gcaatcctga gaacgaagcg
     3901 agcattgagg aactgggcga gatgctgctg gcgagcttcg aaaaacatcc gctgcgccat
     3961 catttcccac cgtttgcggg ctttcgcgtt gtcgaaagta gcagctacta cggcaaagga
     4021 tatcaggacg tagagcatcg taaaccgagc atccgcaatg cccaccgctg cctggactgg
     4081 gagccgaaaa ttgatatgca ggaaaccatc gacgaaacgc tggatttctt cctgcgcacc
     4141 gttgatctta cggataaacc atcatgacca aagtaggctt acgcattgat gtcgatacct
     4201 ttcgtggcac ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag cataatattc
     4261 aggccagcat ttttttcagc gtcggcccgg acaatatggg ccgccatctc tggcgactgg
     4321 tgaagccaca gtttttgtgg aagatgctgc gctcaaatgc ggcatcgctt tatggctggg
     4381 atattttact ggcaggtacg gcctggccag gtaaagagat tggtcatgcc aatgccgata
     4441 tcattcgtga agcggctaaa catcacgaag tcggcctgca cgcctgggat caccatgcct
     4501 ggcaagcccg tagcggtaac tgggatcggc aaacaatgat cgacgatatt gcacgcggtc
     4561 ttcgcactct ggaagagatt atcggtcaac cggtaacctg ttctgccgct gcgggctggc
     4621 gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca tttgcgctac aacagcgatt
     4681 gtcgtggggc catgccgttc cgtccattgc tcgaatcagg aaaccctggc actgcgcaaa
     4741 ttccggtgac cttacccacc tgggatgaag tgattggtcg ggatgtgaaa gcagaagatt
     4801 ttaacggttg gttactcaac cgcatcctgc gagataaagg cacgccggtt tataccattc
     4861 atgcagaagt tgaaggctgc gcttatcagc ataattttgt ggatctcctc aaacgcgcag
     4921 ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt atcagagacg ttgccgctcg
     4981 gacaagttgt tcgcggaaat attgccggac gtgaaggctg gctgggttgc caacaaattg
     5041 cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg
     5101 ctattacctg ttaccgatca gcacgcgtct gctctggcaa cccgatgaaa cgcgttatgc
     5161 ggaaatcagt cgagaaatgc tggcatccgg cgactggatt gtgccccaac tgttagggct
     5221 acgttatttc gaaaaaccca ttgccggata ctggattaac agcattgggc aatggctatt
     5281 tggcgcgaat aactttggtg tgcgggcagg cgttatcttt gcgaccctgt taactgccgc
     5341 gctggtgacc tggtttactc tgcgcttatg gcgcgataaa cgtctggctc tactcgccac
     5401 agtaatttat ctctcattgt ttattgtcta tgccatcggc acttatgccg tgctcgatcc
     5461 gtttattgca ttctggctgg tggcgggaat gtgcagcttc tggctggcaa tgcaggcaca
     5521 gacgtggaaa ggcaaaagcg caggattttt actgctggga atcacctgcg gcatgggggt
     5581 gatgaccaaa ggttttctcg cccttgccgt gccggtatta agcgtgctgc catgggtagc
     5641 gacgcaaaaa cgctggaaag atctctttat ttacggctgg ctggcggtta tcagttgcgt
     5701 actgacggtt ctcccttggg gactggcgat agcgcagcgg gagcctgact tctggcacta
     5761 ttttttctgg gttgagcata ttcaacgctt tgcactggat gatgcccaac atagagctcc
     5821 gttctggtac tacgtgccgg tcatcattgc cggtagcctg ccgtggctgg gattactccc
     5881 cggtgcactg tacacaggct ggaaaaaccg caagcattcc gcaaccgtct atttgttgag
     5941 ctggacgata atgccgctgc tgtttttctc cgtcgctaaa ggtaaattgc ccacctatat
     6001 tctttcctgc tttgcatctc tggcaatgct gatggcgcat tacgctttgc tggcagcaaa
     6061 aaataatcct ctggcgctgc ggattaatgg ctggattaac atcgcttttg gcgtcactgg
     6121 cattattgcc acatttgtgg tctccccgtg gggaccaatg aacacgccgg tgtggcaaac
     6181 cttcgagagc tataaagtct tttgtgcctg gtcgattttt tcgctatggg catttttcgg
     6241 ctggtacacc ttaacaaacg tcgaaaagac ctggcctttt gccgcgcttt gcccgctggg
     6301 gctggcgttg ctggtaggat tttcaattcc tgacagagtt atggaaggaa aacatccgca
     6361 attttttgtc gagatgacac aagaatcact tcagccaagc cgctatattc ttactgatag
     6421 cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc gatgacatca tcatgtatcg
     6481 ccagacaggt gagttgaaat acggccttaa ttatccggat gcgaaaggga gatttgtcag
     6541 cggtgatgag ttcgcaaact ggcttaatca acatcgtcag gaggggatta ttactctcgt
     6601 gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc attccgcccg cagatgccat
     6661 cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc aaatgatctg gctaacatta
     6721 gtctttgcca gcttgcttag cgttgccggg cagttgtgtc agaaacaggc aacctgcttt
     6781 gtggcgataa acaaacggcg caaacatatc gtgctgtggc tgggactggc gctggcttgt
     6841 cttggtcttg ccatggtgct ctggctgctg gtcttgcaga acgtaccggt aggcattgcc
     6901 tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac
     6961 gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg cattgtgatc
     7021 ctcgggagta cggtgtaatg ggcctgatgt ggggattatt cagcgtcatt attgcctcag
     7081 ttgcgcaatt aagcctgggt tttgcggcga gtcatctgcc gccgatgacg cacttatggg
     7141 attttatcgc ggctctgtta gcttttggcc tggatgcccg gatactgtta ctcgggctgc
     7201 tgggttatct gctttccgtt ttttgttggt ataaaacgct acataaactt gcgctaagca
     7261 aagcctatgc cttgctcagt atgagttatg tgctggtatg gattgcttca atggtacttc
     7321 ccggctggga agggactttt tcgctgaaag cactactggg agtagcctgt attatgagcg
     7381 ggttgatgct gattttcctg cccatgacaa aacaacgtta ctgagttttc cctgccactt
     7441 tacactgccg ttcccactca tcggggctat aactgcttgc cgagaggact ttcaccgtat
     7501 gcagcttttc acgattaata caataaagcg cattctgcaa aggcgagagt aaatccccca
     7561 ctttcacggc gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg ccagcatcgc
     7621 agagcataag tacatgtctg ttatcttgtt tattgcagca cgattttttg accagccatt
     7681 ccattgcatt atcctgtttg ctaagagttt tcacatcaat ttacagatta acagataaac
     7741 tctttatcaa cggcaatgga atggcagaaa tgagaagtga aacggagggt taattattgc
     7801 tgacgttgca cccactcttt tagcgcctga cgtgaaattt taataccgcc gtttttcagt
     7861 tcaggcggca acgttaacca acgcaccggt tgctgaaaac tggccagctt atctttcacc
     7921 cattcactaa gatcaacgct ctcgtggtca tactccatca ccgccaccgg tcgatgacca
     7981 aactccttgt cggcaacggg gacgataaac acctgcaaaa ccgcaggatg tgcagcaatt
     8041 acgcgctcga cttcttccgg ctgaataccc tcaccgccac tgaagaacag attgtctaaa
     8101 cgtccgacaa tggtcagctt gccattatgc atctcaccgc gatcgcgcgt agcgtaccag
     8161 ccttcgtcat taaccagtga aaccagttgc ccgttacgcc agtaaccttc tgccatactg
     8221 gcagcccgca gccacacttc attattaacg attttcactt cccgacccgg cagcggcgaa
     8281 ccaacgtctg ctaggccgtc ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga
     8341 ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg
     8401 atagccgcgc cgccaagtaa caccgctttc agggaaacgg aactgcggtt aaccagcaaa
     8461 cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat ttgctccaat
     8521 ggctgtttat cacgcaccgt catccgcgca ccagcgtata accagcgcca cataattccc
     8581 tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgatc gccaaacgga
     8641 atcaacgata acaccccttc ggcactggca agatgggcct gataagtatg gacagcggct
     8701 ttcggcaacc cggtagaacc cgaggtcaac gtcattgagc acagacgcgt cggctgccac
     8761 gtagcggcat gtgcgccttc aacccgctga atgtgcagcg acgttaacgc cggaaacgtg
     8821 ttttccccat ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc
     8881 aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccgcattg cagtaacgcc
     8941 agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca
     9001 acaacgccct gcgccgcaaa gcctgaggct aattcatcga cgcgagcaca aagctcgcgc
     9061 cagttaagtt gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccag
     9121 tgacgccacg gccagtcaga gaagatcata acaaccgctc cagtgcatca acttccacga
     9181 caggcagcgt gctacccggc cagcgacgta cctgctgcgc ctgcatcaga tccagcgtgt
     9241 ccagccctgg aatggtgtcc ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc
     9301 ctaagctcga ttcaatggaa gaactgatca ccgccgtcag ccccagcgcg tgcgccgcct
     9361 gtacctgctc gcgtactttc tccagactgc ccgtgagcgt gggtttgata actaccgcgc
     9421 gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg caagctttca tcccaggcaa
     9481 tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg cacggctctt
     9541 cgagaaacgc gatgcggtgg cgataatccg ggttaacata tttggcaaac tgctggcctt
     9601 tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca
     9661 gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta caatcccact ttgaccttcg
     9721 ccactttctc gcctggcata tcagcaagtt tgaggatcag atcgtcagga tcgccattac
     9781 acagtggtgc cgcacggtaa ttggccgcct gcgggagcgt atctgccagc tccgccaatg
     9841 cacagcttac gccaaaggca acggaaggca tctgcggtag ctcgcaatcg cctgccagcc
     9901 agttatttac ccaggcaagc agcacgcttt gcgcctcttc ccaggtttcc tggctgaagc
     9961 ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg caaacgtaca
    10021 gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct
    10081 gccagcggta tacctgcgcg ctacgcatta cggattccgt ttaaatttgc tgaaatcagg
    10141 ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct tcttccgtca tgtagaacag
    10201 catggtggcg ttgcccgcca gctcctgcag ccccgcctgc ccgtcacagt cggcgttcag
    10261 cgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg
    10321 gacggtttct ttttccagat ccgccagcgg taccacggtg ttgacaaggc ccatatccag
    10381 cgcctgtttt gcgtcgtact gacggcacag gaaccagatt tctcgcgctt ttttctgccc
    10441 gacgatacga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc
    10501 agtctgaccg aagatggcat tatctgctgc gatagtcagg tcgcacatca tgtgcagaac
    10561 gtgaccaccg ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg
    10621 gatctgacgc tggaagtcca gcacgttcaa gtgatgtacg ccggaatcat ctttatagcc
    10681 gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc
    10741 agtcagaatg atcacgccga tattgtcgtc ataacgcgca tctgccagtg cctggatcat
    10801 ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt
    10861 tgcgataccg tcggtggatt tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg
    10921 ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa atcatattgt gtcctttagt
    10981 cagaaacgca agatctgcgc cagacttgcg attacgccag cgggattttc ccgatgcgcg
    11041 ttatgtccgg cgcgaggaat gacatggcag tcggcagcca gttccgccgc cagggcgcgg
    11101 aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta
    11161 gcacgtaaat caggctggac ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg
    11221 ccattattgt tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca
    11281 aaaacaggct gttgatacca gtcggcaaat accgccgtta acggttctgt gcgaaaacgc
    11341 tgcgcccatt ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt ttgcagcccc
    11401 ggatgcccgc cttcgacaat aaccccacaa agccccgcca gcccctggca agccgccatc
    11461 atcgccaccc gtccgccaag cgagtacccc actagccaaa agtcaaggat gttgtaacta
    11521 accaaggttt tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc
    11581 gaaccaccat gacctgggag atcaacatac aaccgtgagt agtcggcaaa cgcctcgccc
    11641 acttcttgcc attcgtggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa
    11701 cctggttttc cgtgttttgc ctgcgcgtgc aggatcataa atggcttacc tgcgccagaa
    11761 gttgctggag cgtttgcgca ccatcggtgt cgttaaccac catttcaatc accgtggtgg
    11821 ttggcgtgcg ccaggcgtcg gcaaatgccg tttcaagttc ctgccagttt tgcggacgat
    11881 gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat
    11941 agaaacgctc acgctcgctt tgcggcgttg gcaacagcga gaaaatttgc ccgccgttgt
    12001 tgttcaccac aattaatacc agcggcgcag aaacctgacg caataacgcc agcgcgttga
    12061 gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct
    12121 gaacgcctgc ggcggtcgaa agcagtccgt cgataccgct ggcccctcgg ttgctgtaca
    12181 ccgggtaacc tgctggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac
    12241 caacaaacaa ttgcccctgt tcaggcagat agtcgcagat gcgatgcgcc agttgcgctt
    12301 cgccaaacgc atcacggcgg gcaataaccg cctgcattgc ctgttccgcc aggcgcggga
    12361 tttcaacgca ccagggctgg cgtttttctg ccggatgcag ctccagccag tcggcaatat
    12421 tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag tcgcccttca atgtcatcaa
    12481 caatccagta ctcttctggt tcacagcttg cctgccattg caggagccgt ttgcccgtca
    12541 ggctgcttcc cagttgcacc acaatttgcg cctgctgcag ctcgctggtc gctttggcat
    12601 tgcctaacca aagatcggca cacggcagcg gctgcccggt ttgtgacagc acatcgccaa
    12661 tcagcggcca gccaagagtt tgcgcccaca gggcaacttt tttgccctct tccgcactca
    12721 tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg cgctgttttt
    12781 cactttccag acgaggcgct tcacgcagcc acggtttgtc gtcctgccac cagtcaccca
    12841 gacgctgttg ccagctaagc ccggtatcgt ccatttcgcc atacagcggt tcagcaaacg
    12901 ggcagttgat atggactccc cccgcatgaa gcgtaccgag agcgtggtcg atggtagaaa
    12961 ccagccaacg tgcggggata tcccgggtcg ggcgcggcaa tgaaatactg tgcgtggggt
    13021 gagaggcgaa cattcccggc tggcgaattg cctgattcgc gccgcagtca attagctccg
    13081 gcgggcgatc ggcggttaac aggatcagtt tttctccggt taacccggct tcaatcagtg
    13141 caggatagag atttgctacc gccgtgccgg aggtcacaat caccgccacc ggctgcttgc
    13201 tgactttcgc cagccccagc gccagatgcc ccaacccacg ctcatcgaaa tgggtgtggt
    13261 gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagccagggg
    13321 cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc
    13381 gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata
    13441 aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc
    13501 atttgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg
    13561 gggtcggaac cacggacaat gcccgcgcca gcatatagcc gcacgacatt gccgctgatt
    13621 tttgctgaac gcagggaaac gcagaattcg ctttgttgta atgagagata gcccgcagaa
    13681 ccggcgtacc attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg
    13741 cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacatcatcc
    13801 gctttgttga gtgaagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt
    13861 acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg gcagatatct
    13921 tccaccacca gcatgttctc gcgctggttt ttatcatccg ccatcagcca ctctcctaac
    13981 tgctgcgcct gcttatcatc aggattattt gctactgttc ccgccagcgc ttcagtacgc
    14041 agcgctttgt cacgccgccg ccataaccgt tccggtgaag agccaagaaa agcattttcg
    14101 ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca gtcgacgact ggcagccatc
    14161 atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc gagcaccact
    14221 ttgtcgagct ccccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc
    14281 gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg caagggcttg
    14341 atactcacca gtgtggcgat aaattctctt gcctgaatcg catcgtgctg aagggagctt
    14401 tcgctgaata gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca ttccaggcgt
    14461 ggtaaaagta aattgccctg cgacgggtca aacgcattca gcccccaaat gcgtaagtcg
    14521 gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta
    14581 atcgcgccca ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcggg
    14641 tacgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga
    14701 atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga
    14761 ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt
    14821 atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc
    14881 agcaacagtc ctagtaccag cccgacggcc gcgcccacac caattccttg ccagggtttt
    14941 tcgtggacgt agtcatcagc acgataaaca gcctgcttcg cccgatagta ataactgtct
    15001 gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc
    15061 tcaacatatt tctgatcggc gggatcgcca gaggagcgga gcacctcttc cagtgtttca
    15121 ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa actgattaga cattctcgtt
    15181 ctccatttgc gtaaaacctg tcactaacta tagacaaggg ttgttgatta cgcctgaatt
    15241 acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc cgtcacaggg
    15301 ataaaaccga aactctggta gaagttttgc agatgcgcct gcgccccgag gtaaacaggc
    15361 ttatcaggcc agtgatgcgt gcaggtgtcg agggttttac tcatcagttg ctgccccact
    15421 ttttcgccac gcaacgcttc actgacaatc acccgaccta taacgaccgg ctcaagatca
    15481 tcatcacttt tcagaatcct cgcatacgcc accagttcat catttttcca accgagaata
    15541 tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctcg
    15601 acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca
    15661 gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac
    15721 tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttca
    15781 gccggtgtgc caacccgcac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg
    15841 acccagagcg gactttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat
    15901 accgccttta accctggaaa actggacaag atttttatca gtcaccttca tggcgatcat
    15961 ctttttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta
    16021 acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa ccgcgctgcg gattagcggc
    16081 tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat
    16141 ggcctgcgca aagtaaccgc ttatccgctg gaacacccac tggaatgtta tggctatcgt
    16201 attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg
    16261 ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa caatcacgct ggaagatgga
    16321 aggcagatta acggcgcaga ttacttagct gctccagtgc caggtaaagc gctcgctatt
    16381 ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg
    16441 gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggccatagc
    16501 tctacacgcc aggctgcgac actagcccgt gaggctggag tcggtaagct aatcattacc
    16561 cacgtcagct cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga atgcaggtca
    16621 attttcccgg cgactgaact agcgaatgat ttcaccgtgt ttaacgttta actctgctta
    16681 ttattaaaaa cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat atatccaccc
    16741 acacgggcat attgagcgat ttttgatatt tattcatctc cttacgcact atattcagag
    16801 caataagttg cctcttttta tcttcattca gcacaacctt tgcaactaaa aggagcccat
    16861 gatggttaca gttgtcagca attattgtca attatctcaa acccaactca gtcagacatt
    16921 tgcagaaaaa tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc
    16981 cggagatgaa gaaagtattg agttactgca taatattgcg ttaggttatg atgaatttgg
    17041 aaaaaaagct gaagatattc tttaccatat tgttagaaac ccaacaaatg agaccctatc
    17101 gattatccga cttattaaaa atgcctgttt aaaattatat aatttggcac ataccgcaac
    17161 caactcccac cttaaaccaa ctggtccgga taattcagat gttctcctgt tcaaaaagct
    17221 attctcccct tcgaaattaa tgacaattat cggtgatgaa attcctctta tatctgaaaa
    17281 acagtcgctt tcaaaggtgc ttttaaatga tgagaataat gaactgagtg atgatacaaa
    17341 cttctgggat aaaaatcgtc aattaaccac agatgaaata gcttgctatc ttcagaagat
    17401 cgccgctaat gcaaaaaata ctcaagtcaa ttatcctact ggtctctacg tcccctactc
    17461 caccagaact cacctggaag acgctctcaa tgaaaatatt aagagcgatc catcatggcc
    17521 gaatgaagtc cagttattcc ccataaatac tggcggacac tggatattag tttcgctaca
    17581 gaaaatagta aataaaaaaa ataataaact acaaataaaa tgcgtcatat tcaactcatt
    17641 gcgtgcacta ggccatgata aagaaaattc acttacgcgt gtcattaata gttttaattc
    17701 tgaactcatg ggagaaatgt cgaataataa tataaaagtt catttaaatg aaccagagat
    17761 aatattttta catgccgatc ttcagcaata cttaagccaa agttgcggtg cgtttgtgtg
    17821 catggcagcc caggaagtga ttgaacaaag ggaaagcaat tctgacagcg ccccctatac
    17881 gttattaaaa aactatgctg acagatttaa aaaatattca gcagaagagc agtacgaaat
    17941 tgattttcaa catcgactgg taaacagaaa ttgttattta gataaatatg gcgatgcacg
    18001 aatcaatgct tcttatacac aattagaaat aaaacactca caacccaaaa atagagcatc
    18061 cggcaaaaga gtgagttaac atcattgtta attaattcga ccagtcagca aatatgctga
    18121 ctggtcgaac atagtttgct aaacagtcat cactgactga tgtcagtcac accatccgcc
    18181 agttcaatct tcgggtattt ccagcgtatc tcggcacgcg attgctggtc tgttgatgta
    18241 gtggattgtt gttgctgact tttcttaccc tcagagttct gccccctgtc aactcaatct
    18301 gccaataaat aacataatta tatttttcct tatatgaatt cctttttaaa atagcgaagc
    18361 cactgtaa
//
LOCUS       DAZFYC010000096        18345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFYC010000096 DAZFYC010000000
VERSION     DAZFYC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.096"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..594
                     /locus_tag="ACHKW5_003651"
                     /pseudo
     CDS             1..594
                     /locus_tag="ACHKW5_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            697..1200
                     /locus_tag="ACHKW5_003652"
     CDS             697..1200
                     /locus_tag="ACHKW5_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO7512975.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1267..2724)
                     /gene="nuoN"
                     /locus_tag="ACHKW5_003653"
     CDS             complement(1267..2724)
                     /gene="nuoN"
                     /locus_tag="ACHKW5_003653"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO7512976.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2731..4260)
                     /gene="nuoM"
                     /locus_tag="ACHKW5_003654"
     CDS             complement(2731..4260)
                     /gene="nuoM"
                     /locus_tag="ACHKW5_003654"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO7512977.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4424..6265)
                     /gene="nuoL"
                     /locus_tag="ACHKW5_003655"
     CDS             complement(4424..6265)
                     /gene="nuoL"
                     /locus_tag="ACHKW5_003655"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO7512978.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6262..6564)
                     /gene="nuoK"
                     /locus_tag="ACHKW5_003656"
     CDS             complement(6262..6564)
                     /gene="nuoK"
                     /locus_tag="ACHKW5_003656"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO7512979.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6561..7115)
                     /gene="nuoJ"
                     /locus_tag="ACHKW5_003657"
     CDS             complement(6561..7115)
                     /gene="nuoJ"
                     /locus_tag="ACHKW5_003657"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO7512980.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7127..7669)
                     /gene="nuoI"
                     /locus_tag="ACHKW5_003658"
     CDS             complement(7127..7669)
                     /gene="nuoI"
                     /locus_tag="ACHKW5_003658"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO7512981.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7684..8661)
                     /gene="nuoH"
                     /locus_tag="ACHKW5_003659"
     CDS             complement(7684..8661)
                     /gene="nuoH"
                     /locus_tag="ACHKW5_003659"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO7512982.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8658..11384)
                     /gene="nuoG"
                     /locus_tag="ACHKW5_003660"
     CDS             complement(8658..11384)
                     /gene="nuoG"
                     /locus_tag="ACHKW5_003660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO7512983.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11437..12774)
                     /gene="nuoF"
                     /locus_tag="ACHKW5_003661"
     CDS             complement(11437..12774)
                     /gene="nuoF"
                     /locus_tag="ACHKW5_003661"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO7512984.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12771..13271)
                     /gene="nuoE"
                     /locus_tag="ACHKW5_003662"
     CDS             complement(12771..13271)
                     /gene="nuoE"
                     /locus_tag="ACHKW5_003662"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO7512985.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13274..15076)
                     /gene="nuoC"
                     /locus_tag="ACHKW5_003663"
     CDS             complement(13274..15076)
                     /gene="nuoC"
                     /locus_tag="ACHKW5_003663"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO7512986.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15170..15832)
                     /gene="nuoB"
                     /locus_tag="ACHKW5_003664"
     CDS             complement(15170..15832)
                     /gene="nuoB"
                     /locus_tag="ACHKW5_003664"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO7512987.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15848..16291)
                     /gene="nuoA"
                     /locus_tag="ACHKW5_003665"
     CDS             complement(15848..16291)
                     /gene="nuoA"
                     /locus_tag="ACHKW5_003665"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO7512988.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16922..17860)
                     /gene="lrhA"
                     /locus_tag="ACHKW5_003666"
     CDS             complement(16922..17860)
                     /gene="lrhA"
                     /locus_tag="ACHKW5_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO7512989.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4597 a         5087 c         4719 g         3942 t
ORIGIN      
        1 ctctgattat ttttaccaac cctgggtcac tgcattatcg ccaacgcccc gacacgtcag
       61 tcctgagcaa ttagaaaaaa cagttcgtta tcttacacaa actgtgcatc cacgtaatgc
      121 cgacaatatt gataatctga ataggtccgt agagtatata aaagagttct ttgtcagtag
      181 cggtgccaga gttaccgcgc agggcgtccc cgattggcta ttacgactct gcgcctggta
      241 gccaggatta cccttacccg caatgtcctg gctttatccc gatcgggggg actttattgc
      301 ctggtcggca gaatacagga tatcaacgcc gttcgtcagg taaaaacggc attgttatca
      361 tcccgggatt tatctgttta ttctatgaat gccccatggt ttattcccgg tattgatttc
      421 tctgaccacc tgaattattg gcaacacgat attcccgccg taatgattac tgacaccgct
      481 ttttatcgta ataaacaata ccacttgccc ggtgatacag cagacagatt gaattatcag
      541 aaaatggctc aggtggtgga tggggttata actttgttat acaacagtaa ataataaaaa
      601 tttctcgagg cgggtccaga aaaagcgaca tgcacatttt attttccctc gtttatttta
      661 agatgcgaaa cataaaattc aacggaggaa aaataaatgg gaatgattgg ctattttgca
      721 gaaattgact cagaaaaaat taaccaactg cttgagtcta ctgaaaaacc cttaatggac
      781 aatattcatg acactctttc aggattacgc cgactggata ttgataaacg ctgggatttt
      841 ttacactttg ggctgacagg cacctctgct tttgatcccg cgaagaatga tccgctgagt
      901 cgtgccgtgc ttggtgaaca cagcctcgaa gatggcatag atggcttcct gggattgacc
      961 tggaatcagg agttagccgc gacaatcgac cgactggaag ctttggatcg aagtgagctg
     1021 cgtaaaaagt tttccattaa gcgactcaat gaaatggaga tttatccggg agtgaccttt
     1081 tcagaagagt tagaggggca actctttgct tcgatcatgc ttgatatgga aaagctgatt
     1141 agcgcgtatc gacgtatgct gcgacagggg aatcatgcgc tgacggttat tgttgggtga
     1201 tggtgtcaga gcgccaaaaa aaaccgccga acatgtcggc ggctttctga attacgaact
     1261 gacagcttac atcagcggca tggctaaccg cacaatgcta atcagcggtt gtggccatac
     1321 acccagcacc agtaccaaca gtgcagagat cagcacaacg ataccgcccg cgctgtactg
     1381 ccagtttgat ggtgcatcgc gacccggttg ttccggggcg tgaagataca ggctcaccgc
     1441 cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca accacggcac ccaccagcca
     1501 ccacaagtgt gcctggacac cgaccgccag cacgtagaac ttaccgataa agcccagcgt
     1561 catcgggata ccggccagag acagcatcat caccgtcatc actgccgcga ggatcggacg
     1621 atgccagaac agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct
     1681 ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cggccaggta
     1741 aacccctacc gcttccatcg acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag
     1801 atagccgagg tgagagatag atgagtaacc gagcagacgt ttgatgttgg tctggctcaa
     1861 cgccatcagg ttaccgaaga tgatggaggc aaaggcgata atcgccagta ctacgcgaat
     1921 cgcttcgctg tcacccaccg gtgcgtagag gaacagacgc atcaccacac cgaagatagc
     1981 gattttgctc gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc
     2041 tggcgtccac aggtggaacg gcaccagaga gagtttgaag ccgaggccaa caatcatcag
     2101 gccgaaacct gccagcaaca gcggctcgtt gagcataccg tcgccgaggt ttttacccaa
     2161 cgcgacaaac gacaggtcgc cagactgcgc atacaccagc gccataccga acagcaggaa
     2221 tgaagacgct gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg
     2281 gcggaaagcg taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa
     2341 cagagacgcc agatggttgg cattcgccag caggatcccg cccagcgcgg caattaacac
     2401 caacaggtag aactcatcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt
     2461 acaggtggcg aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac
     2521 gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggccg acaaaccaga gcgaaaccag
     2581 cgccgcgtta agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc
     2641 aatggagagc atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat
     2701 caggttttgt ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac
     2761 aaaccactgc tggatattgc caatcgcgga gtgcgaggta tccagaatcg gctgcggata
     2821 gaagcccagc agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga
     2881 catccctggc agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg
     2941 taacatcgcc agcgaataaa cagatgcaaa gaccagccca aaggtagaga taacggtaat
     3001 caccgggaca acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt
     3061 gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca gccatttcat
     3121 cttgctccac agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg
     3181 accacaaaga ataaacagac ccgccgccga caagccgtgc gcaatcatct ggattaccgc
     3241 gccctggtag gccaactggc tgccggtgta gatggcaatc agcacgaagc ccatgtggga
     3301 aaccgaggtg taggcgatca gacgtttgat gtcggtctgg gcgaaggcca tccacgcacc
     3361 gtagaagatg ccgataacac ccagccacat agcgattggc gcaaactctg ccgacgcgtt
     3421 cgggaacagc ggcagggaga aacgcagcaa accgtaagcg gcagttttca gcaagatccc
     3481 cgcgaggtca acggaaccgg cggtcggagc ctgggagtgc gcatccggca gccagccatg
     3541 cagcggaacc accggcattt tgactgcgaa ggcgatgaag aagcccagca ttaacaggta
     3601 ttccacacca ctggacattg gcgtattcag cagctcttca tagttgaagg tccagacgcc
     3661 ggtcgcattg tagtgaacaa aaaccagcgc caggatggcg atcaacatca ccagaccact
     3721 cgcctgggtg taaatgaaga acttggttgc cgccgtgata cgcgttttac cgtcagaggc
     3781 tttatgcccc cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa
     3841 gaagaacagg aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat
     3901 caggttgagg tggaagaagc cctgatattt ttcgatctct ttccacgaac agagtaccgc
     3961 cagcacaccg agcagaccgg tcagcacgac catcagcagc gacagcccgt caatggcgag
     4021 atgaatagag ataccaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg
     4081 aattccggcg gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc
     4141 cagcgtcaat cccatggtga tcagcgcgat ccagcgcggc accttgacgc caaagcgttc
     4201 ggtctgccag cacaggaagc cgccgataaa gggaattaat attagccagg gtagtaacat
     4261 ggcgatcttt attccttgta aaagtcccgt caggaccgga ttttcaacga attctcacga
     4321 cagaaaattc tcttcggact gggggtagtg tcggatgcac cgcgaatgcg gcttatccga
     4381 cctacgttct tatatgccca ggggcaatcc cacaatcctt aactcaacgc agtaccatca
     4441 acagtgccag caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagatagc
     4501 cgttctcact taacagcaga cctttacctg caaagcggga aaggacagcc gggatgttca
     4561 tcattgagtt cagcggatcg cgtttcagca accaggcaat acccaggaat ggcttgacga
     4621 acactttgtc gtacagccag tcaaagcccc aggcgttgta ccaccaggtg cccagcagac
     4681 ggcccggcgc actgttggcg atggaggtca ccagagtacg tttacccagc cacagccagg
     4741 ctgccagcag aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc
     4801 tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg
     4861 cgccaacgaa ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt
     4921 tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg
     4981 tgtagagcga ggtcataaac gcacccacca gacctgccac catcagattg atatgaccat
     5041 tcgccatcgc acccgcgagg atctcatcct tactgaagaa gcccgcagtg accagcggca
     5101 gtgccgacag tgctgcgccg cccaccagga agcagagata aaccagcgga atagatttac
     5161 gcagaccgcc catcttgaag atgttctgtt cgtgatggca ggccagaatg acggaaccgg
     5221 atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat
     5281 cccatgcctg cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag
     5341 cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa accggccagc agcagcgtaa
     5401 ccgccccaac aatgcccacc agatgcagaa cttccggggt catcaggaac aggccgtggg
     5461 tacgggcgat caggtagaca cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga
     5521 caggcgtcgg gcccgccatc gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt
     5581 taccgaccgc accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccatcag
     5641 caaagtgcgc tggtgccagt tccaccattt cgcggaagtt cagggtgccc agttcgttgt
     5701 aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt
     5761 tcattgccgc tgcgccattc ttcggatcgg tgtaatagaa accgatcagc agataggagc
     5821 acaggcccac gccttcccag ccgaggtaca tcagcagcag gttgtcggca agcaccagaa
     5881 ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag ccctcttcac
     5941 cgcgcatgta ccaggaggcg tacatgtgga tcaggaaacc cacaccagtg accactgaga
     6001 gcatggtcag cgacaggccg tccaacacca ggttaaaacc gatgttaaag tcgcctaccg
     6061 acatccacgt ccacagcggc tggctgtatg cctgctcgcc gttagcgaag aaatcaacgc
     6121 cgataaaggc ggttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaaa
     6181 cgttttcaga ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa
     6241 taatggttaa ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt
     6301 ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc
     6361 gaggctgatg gcgagaatgt acatcacctg accgtcggtc tgaccccagt agcttccggc
     6421 gaccacaaag gccagcgcgg aggcgttaat catgatttcc agaccaatca gcataaacag
     6481 cagattgcga cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat
     6541 cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct
     6601 ttacgattgc tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc
     6661 agacccgcga gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc
     6721 gtaataccca ctgctttagc actgattggc gtaccgtcga taccctgatc gttaacaccg
     6781 aggatggcgt aaacaatcac caccagcatg atggccgaca aaattgccgg accaatccac
     6841 acctgcggtt tcagccactg gcgttcctgt tcgatttctg aaccgcccag gttgagcatc
     6901 atcaccacga acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca
     6961 ccggcgaagt aagcgcctag tgagaagaac accccggaaa tcgccagcag cgaaataatc
     7021 aggtacagca gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg
     7081 gctatcaggc cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc
     7141 tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg
     7201 ccatacctgc catccggtag aagttatatt ccgggtattt gcccggaccg gagatcagca
     7261 gatcctcttt ctcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg
     7321 ttaactgaat cgccgtggtc ggacaggctt cttcgcacag accacagaaa atgcagcgtg
     7381 agaagttgat acggaaaaat tccggatacc agcgaccgtc tttggtttct gccttttgca
     7441 gcgagataca gccgaccggg caggctaccg cacagaggtt acaggctacg caacgctctt
     7501 cgccgtccgg gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg
     7561 gctcttccgg gtacattcgc gtttcgcgtt ttgcgaacgc gtgcaggccg atcatccaga
     7621 tactacgaac ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc
     7681 cccttattgc gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag
     7741 cggcaggcag attttccagc cgaaggacat tacctggtca taacgcggac gcggtaacga
     7801 cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa
     7861 tggcggtaac aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat
     7921 ggtcacaatc ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc
     7981 aatgtggtaa ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg
     8041 acacaccgcc acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggatgacgtt
     8101 ccacacatgc gcctggctgt tgacgatgtc ggtcatattg aatgaaccgg cctgcgccac
     8161 cacgcccatc agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg
     8221 catcgcaccg agcagcgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta
     8281 aactgccaga cctgccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac
     8341 ccaacccgga ctgactggca caattgcaaa ggccagcagc agcgaggtaa aggcaatcat
     8401 cggtgccagg gtaaagatga cgcgatccga gaacttcggg atccagtctt ctttaaagaa
     8461 cattttgatc atatccgcaa ccaactggag cgaaccgccc cagccaacac ggttaggtcc
     8521 gtaacggttc tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccgca
     8581 ggtgacaacc accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag
     8641 ttccggtgat atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc
     8701 agcaccggag caatgccgga catacccatc ggcaagccca cctgccctgc cgtcagccct
     8761 tcggcgattt caaccggcag cgtgaccgtg ttgccatcgt aactaaagga gacgcgtgta
     8821 cctgcgttta cacccaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc
     8881 atacgactct ggaagaccgg agcacgctgt gacaattcat cactgccaaa caggtggtag
     8941 tacggcgcaa tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag
     9001 taatccagac cattttcgct ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt
     9061 ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggagcc
     9121 caggcaaacg gcacttgcga acggtgcgcg gtcggctggt tgttaccttc catcgagaag
     9181 gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc
     9241 atggcggtac ggccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg
     9301 aatgtcgcat ccggcgcagc atctttgata cctgccagtt ccgggatttt cgccacaaca
     9361 gcgtcaatca catggtcgag ctgcgtccag tccacttcac ggctcagcag ggtgctgtgc
     9421 agcgagtgta accaacgcca gctttccagc atgacagttt tgctatcgta ataagccgga
     9481 tcgtaaacct ggaagaaacg ttgggcgcga ccttcattat taattaccgt accgtcgctt
     9541 tcagcaaagc tggcagcgga aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga
     9601 tgatcaacca ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcggaa
     9661 gcgtgacgat gcagatcgtt ttccagcacc accaccgcgt cggcgcgtcc ggtttccagt
     9721 tcggtcaacg cttcttcaag cgaaccgcca cccataatgc ccagccccat gctgttgacg
     9781 gaacgggcaa tcatggtgat accaacgtca gcgccgcgac ctttcagggc tttcgcgacg
     9841 ttagccgcag cctgaatcac ctctaagcta ccggcgttcg tcccggagat aatcaacggt
     9901 ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt tgctttgcag ctcaggttcg
     9961 ataccgtcaa ccgctggtgc agagttatcc agcgcatggg caatggcaaa acctaaacgc
    10021 gcctgatctt caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgagtgtca
    10081 tcaacgttgg taacaaacag cggatgctta gcacgttgac cgatgttgag gattgccgca
    10141 atctgccagt cagccacttt ctgtgctgcc gccatttcgc gcgctttacc tttcaccgcc
    10201 tgacgcactg ccagcgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt
    10261 accgcatcgt aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc
    10321 actttcagcg ccagttgcag acgttcctgc tcaccgtgag cgataccggt gtagaagttt
    10381 tcttcgccca ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca
    10441 ataccgatca ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg
    10501 gcgttgaggg taatgaaatc atcgccacga cgctgtactg gctgacgcgg acgatccttc
    10561 aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta
    10621 ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac gttcaccggg gctgatgtta
    10681 caaccgatgg aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg
    10741 taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta
    10801 ccggaaaatt cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc
    10861 gcaccgtaaa cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg
    10921 tagcaggcga tgcagcggtt catttcgtga gagatgaatg gccccaaatc ctgattacgg
    10981 tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc
    11041 tgaagatggc agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt
    11101 aaccactcga ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag
    11161 gtgccatcgg aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg
    11221 ttttggtatt gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc
    11281 cagcaaaagt aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct
    11341 ccgttgacct cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt
    11401 ttctcggtca gggaccgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt
    11461 cggctgaatc ccattaatca aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa
    11521 ttcttcgcgg aaatatttga tggcgctctg taacggctcc actgcaccag gtgcgtgggc
    11581 acagaaagtt ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatctcc
    11641 cggctgacct tcgccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc
    11701 gcggcacggc gtacaccagc cgcaggactc acgggcgaaa aactcttcca gattacgcac
    11761 cagcgacacc atgttgatct catggtcaac cgccatcgcc agcgccgtac ccagacggct
    11821 tcccgcttta ccgatacttt cgaattccat cggcaggtca aggtgcgctt cagtcaggaa
    11881 atcagtcccc gcgccgcctg gctgccaggc tttgaatttc agaccgtcac gcataccacc
    11941 ggcgtaatct tcaaggattt cacgcgcggt agtaccgaac ggcagttccc acaggcccgg
    12001 atttttcacc cgaccagaga agcccatcag cttggtgcca gcatctttac ttttcgagat
    12061 gttctgatac cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac
    12121 gttgttgacg caggttggtt taccccatgc accggaggtt gccgggaaag gtggcttcga
    12181 gcgtgggtta gcgcgacgcc cttccaggga gttgattaac gctgtttctt ccccgcagat
    12241 gtagcgccct gccccggtat ggacgaacag ttcgaagtca aaacctgttc ccataatatt
    12301 tttgccaagc aaacctgctt cggtggcttc ggcaatggca cggcgcagat tcactgccgc
    12361 ttcgatatat tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga
    12421 gataagcata ccttccacca gcaggtgcgg cagttgctcc atcagaaggc ggtctttata
    12481 ggtgcccggc tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc
    12541 tttcggcatc aggctccatt tcaggccggt agaaaagccc gcgccgccgc gccctttcag
    12601 accagcgtct tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg
    12661 cgccccttcg taaccgtttt tgctgcgata ttcgtccagc cacactggct gtttgtcatc
    12721 gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac
    12781 cgctccagca gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc
    12841 atcatgtttg gccctttatc acagttcccc aggcagcaag ttggcagcag cgtaaagcga
    12901 ccgtcaaatg tcgtttgccc tggtttgatg tttagctttt tctcgagcgc cgcctgaata
    12961 ccctggtaac cgttgatatg acagaccacg ctgtcgcaat aacggatcac atggcggcca
    13021 accggctggc ggaagatctg gctgtagaac gttgccacgc cttcgacgtc gcttgccgga
    13081 atacccagca catcggcgat cgcgtggatc gcgccatccg gcacccagcc acgctgcttc
    13141 tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg
    13201 tgctcaatcg cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga
    13261 ttctcgtgca taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa
    13321 caatcaggtc agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat
    13381 aactcggcgt gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt
    13441 aactgttgat ccccttcgtc gcctcaatca tctggaaaga ttcattggca ggcatcaccg
    13501 gcccccacga cacttgcaag aagtgggtga tcagggtttc gatatgttgc agcgtgcgct
    13561 ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt
    13621 tgaggcactg ctcaagaatg cgcagactct ggcgcagctc ttccactttc agcattacgc
    13681 gggtgtagca gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc
    13741 cagaataagg acgcgcctta cgcacgtcga agtcgatccc ggtggcacgc aggcccgcgc
    13801 cggtggtgcc ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac
    13861 ctttcagaat ggtattttgc agcgctgctt tctcgtaaga cgccagacgt ttcggcatcc
    13921 agtcgaggaa ctcacgcagc aggcgatccc agccacgcgg caggtcgtgc gctacgccgc
    13981 caatacggaa ccacgccggg tgcatacgga aaccagtgat tgcttccacc agatcgtaaa
    14041 ttttctgacg atcggtaaag gcgaagaaca ctggcgtcat tgcgccgacg tcctgaataa
    14101 aggtcgagat atacagcagg tggctgttga tgcggaacag ctcagaaagc attacgcgaa
    14161 tgacgttaac gcgatccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag
    14221 gcatttcgtt aacgcagccg ccgaggtatt cgatacggtc ggtatacgga atgtagctgt
    14281 gccaggactg gcgttcgccc attttctccg caccacggtg gtggtagccg atgtctggta
    14341 cgcagtcgac aatctcttcg ccatcgagct gcaaaacgat acggaaagca ccgtgcgccg
    14401 acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc gttttcagta ccacgcttca
    14461 tcccccactc ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg
    14521 tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta atctttacgc agcgggtgac
    14581 ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aatgtaatgc
    14641 caaacagatc ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg
    14701 tcggtacgtg caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat
    14761 cgatagaaat cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg
    14821 tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt
    14881 tctttaagaa atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc
    14941 cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa
    15001 ttaccggatc atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg
    15061 tcatattgtt caccattgca aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa
    15121 atagatttag gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt
    15181 caggtgtacg caggttggtt acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt
    15241 gcatattggc gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt
    15301 tgccgataga ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc
    15361 agcccgggat atacacatca accgggatga atttatcgac gccctgcaca acggaataaa
    15421 tatcgtacat accaccagag ttggcacagg cacccattga gataacccat tttggttcca
    15481 gcatctggtc atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa
    15541 ccaccatcag gtcagcctga cgcggggaag cacgcaatac ttctgcgcca aaacgcgcca
    15601 cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt
    15661 tatacggcca aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa
    15721 acacgttttt gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg
    15781 ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc
    15841 ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc
    15901 gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca
    15961 gtaacacaaa aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag
    16021 aggttgacca tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca
    16081 ccagataaaa cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt
    16141 caaacggcac gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca
    16201 gcatcaggca acacaggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag
    16261 cgatgacttc agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt
    16321 cactgctctt cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct
    16381 tacaatcact gtagaaattc ttttatacag ctaattgatg tggtctttta ctcctttcta
    16441 taaccttttg tcaactttaa caaaagtttc ttcacattag tttacataat atcaacacca
    16501 ttagcattta atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt
    16561 tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt
    16621 gtcgattcct ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattcc
    16681 ataacaaaaa cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt
    16741 tttcaggcga ttatttgtgc gtacggggcg tgaatctctg gtgattgaaa aatgaacagt
    16801 tttgtacgtt ctgcacgatg tgaagaaggt tctttactaa aaaaaagccg ctggggttta
    16861 aaacaccccc agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg
    16921 attactcgat atccctttca atcaacaatg aatcatcccc ttccggagca gatattgggc
    16981 tgtattgcca cggattatgg tagctctcca tcgcctgata gatcacctga gccagctcat
    17041 tattactgga cgggtcatag cacagcaaat attcggtatc gggtagcggc ggtaagccat
    17101 cgactccact cagcacacgc aggtctggac tcatcatctc gaccggcctt gccgtcacgc
    17161 caagaccggc tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc
    17221 gccagggaat atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat
    17281 catccagcaa tacaagaggg atcggctcac ctttttgcaa tacgtattcc gctgcgcagt
    17341 accagtgtgt tggcgaggta cgcaggttca atgccttaaa cgctgaagga cgatgggtgg
    17401 ttaccatcaa atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa
    17461 cgcggacatc cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta
    17521 agatcgtatc ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga
    17581 acattaatga tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa
    17641 gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc
    17701 caacgagttg ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag
    17761 cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat
    17821 cgaggtcgag gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg
    17881 ctcatactga gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc
    17941 ctccctggca gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa
    18001 aaaagcgact cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag
    18061 ggatcgcata tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat
    18121 aaatgtaaat gtaagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac
    18181 gtacaaacag caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt
    18241 ttaccccaaa aaccttttat ttataagggt cattgcgaat tatctgatgc aaagttatgt
    18301 tatgttaggc aaagtaatct tctattatta ataagcacat caaaa
//
LOCUS       DAZFYC010000097        18279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFYC010000097 DAZFYC010000000
VERSION     DAZFYC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.097"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(55..630)
                     /locus_tag="ACHKW5_003667"
     CDS             complement(55..630)
                     /locus_tag="ACHKW5_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7512990.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKW5_003668"
     CDS             complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKW5_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO7512991.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKW5_003669"
     CDS             complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKW5_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO7512992.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2389..2844)
                     /locus_tag="ACHKW5_003670"
     CDS             complement(2389..2844)
                     /locus_tag="ACHKW5_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7512993.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2867..4024)
                     /locus_tag="ACHKW5_003671"
     CDS             complement(2867..4024)
                     /locus_tag="ACHKW5_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7512994.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4024..4605)
                     /locus_tag="ACHKW5_003672"
     CDS             complement(4024..4605)
                     /locus_tag="ACHKW5_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7512995.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            4928..5986
                     /locus_tag="ACHKW5_003673"
     CDS             4928..5986
                     /locus_tag="ACHKW5_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO7512996.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQEIIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            5996..7138
                     /locus_tag="ACHKW5_003674"
     CDS             5996..7138
                     /locus_tag="ACHKW5_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO7512997.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            7131..7904
                     /locus_tag="ACHKW5_003675"
     CDS             7131..7904
                     /locus_tag="ACHKW5_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7512998.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            7906..8985
                     /locus_tag="ACHKW5_003676"
     CDS             7906..8985
                     /locus_tag="ACHKW5_003676"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO7512999.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            8985..9941
                     /locus_tag="ACHKW5_003677"
     CDS             8985..9941
                     /locus_tag="ACHKW5_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO7513000.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            9952..11160
                     /locus_tag="ACHKW5_003678"
     CDS             9952..11160
                     /locus_tag="ACHKW5_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO7513001.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            11178..11645
                     /gene="terW"
                     /locus_tag="ACHKW5_003679"
     CDS             11178..11645
                     /gene="terW"
                     /locus_tag="ACHKW5_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO7513002.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            11906..12235
                     /locus_tag="ACHKW5_003680"
     CDS             11906..12235
                     /locus_tag="ACHKW5_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7513003.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            12222..12602
                     /gene="tnpB"
                     /locus_tag="ACHKW5_003681"
     CDS             12222..12602
                     /gene="tnpB"
                     /locus_tag="ACHKW5_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7513004.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            12645..13475
                     /locus_tag="ACHKW5_003682"
                     /pseudo
     CDS             12645..13475
                     /locus_tag="ACHKW5_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(13506..15119)
                     /locus_tag="ACHKW5_003683"
     CDS             complement(13506..15119)
                     /locus_tag="ACHKW5_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO7513005.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(15150..15500)
                     /gene="tnpB"
                     /locus_tag="ACHKW5_003684"
     CDS             complement(15150..15500)
                     /gene="tnpB"
                     /locus_tag="ACHKW5_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7513006.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(15497..15922)
                     /locus_tag="ACHKW5_003685"
     CDS             complement(15497..15922)
                     /locus_tag="ACHKW5_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7513007.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            15976..16727
                     /locus_tag="ACHKW5_003686"
                     /pseudo
     CDS             15976..16727
                     /locus_tag="ACHKW5_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         4469 a         4828 c         4667 g         4315 t
ORIGIN      
        1 cggatcgata cccaaaaaaa accccggcat tgccggggtc agactcagcg tcttttagac
       61 attgacgccg tgctgggaag caagcgctga cagaccaccg gcaaaaccct ggccaacagc
      121 tttaaacttc cactcagtac catggcgata cagttcaccg aagaccattg cggtttcggt
      181 tgaggcgtct tcagacagat cgaaacgggc aatttccgtc ccgttgtcgt tgttgtaaac
      241 gcgcatgaag ctgttgctca ccatgccgaa gttttgttta cgcgcttctg catcatagat
      301 ggtaacggca aataccagtt ttttgatgtc tgctgagact ttggtcagat cgattttgac
      361 ctgctcatcg tcgccgtcgc cttcaccggt acggttgtcg ccctggtgct ctactgcgcc
      421 atcagggctg gttttattat tgaagaaaat gaaatgggca tctgacagta ctttaccgtc
      481 ttcacctact gcgaatacgg aagcgtccag atcaaaaccc tgaccatcgg ttacacgggc
      541 atcccagccc aggccaacca tagcgacatt catggttggt gcttctttgg tcagagatac
      601 gttgccgcct tttacgagag aaactgccat ttttagctcc tgcaaacagt tgaatgaggc
      661 tgaattaaca gccccagata tgaaaagtta cttttcgatc aggacgcgtt aatgccgtac
      721 tgagcacata cagatgccag accaccagca taaccctggc ctactgcgcg gaatttccac
      781 tcaccattgt ggcgatacag ctcgccgaac agcatggcag tctcagtgga cgcatcttcg
      841 gtcagatcgt agcgagcgac ttcagtctgg ttatcgtcat taaccagacg aataaacgca
      901 ccggatacct gaccaaagct ctggcgacga gcctgagcat cgtggatggt cacaacgaag
      961 atgatcttgt caacttcaga cgggacggcg tccagtttaa ttttcagcga ttcatcatca
     1021 ccatcgccct caccggtgcg gttatcgccg gtgtgcgtta cggaaccgtc ggatgacgtc
     1081 aggttgttat agaagatgaa atctgaatcg ccgcgcactt tgccgtttga ggccagcagg
     1141 aatgctgaag catccaggtc aaagtcctga ccgtctgttg aacgcgcatc ccagccaagg
     1201 cccaccagga catttttcat tgacggagct gctttactca gggagacgtt cccgcctttg
     1261 gaaagagaaa cactcataaa ataacctctt cgattagtaa ttgttcaggt taacacttaa
     1321 ggggattaac tccccttttc ctcagattca ggtttgcccg ggaacatgac gcttgcgaga
     1381 atgcccagcg ccagtacacc cagtacaaca tacaggctgg ttgttgccgc gatgctgtaa
     1441 ccatgatgcc agatgtgatc ggtcgcattc aggccgagtt ttgccgcgat gaagaacagc
     1501 agcacgataa cggccttctc cagatgaacc aggtactgtt tcagtgcctc aaggacaaaa
     1561 tacagagtac gcaggcccag gatagcaaac atcatggcac tatagacgat aagcggttca
     1621 cgactgacgg caatgattgc cggtaccgag tcaaacgcga acattacgtc cgagagttca
     1681 accacagcca cacacaggaa cagcggggtc gcatacagcg ccgctttttt accacggcca
     1741 atggtgacat cgctgttttc tggtttcgcc agttcagcat ccacttcctt ctggttaagt
     1801 aggaaggcat gccctctgag cttcggccag atagggaaga agcgtttaac catacggtaa
     1861 gccagatgct gggagtaatc ctcaatttca tcatcgtcat caccgctttt aagcatcatg
     1921 accgccgtcc aggcaacgat aatagcgaag acaacttcaa cgtacggccc cagactcagc
     1981 aggctcgtac cgatggcgac aaagatgccc ctgaagacga tggcaccaat gatcccccag
     2041 tagagaacac ggtggcgata acgatccgga acggcgaacc aggagaagat tgccatcatg
     2101 acgaacaggt tatcgaccga cagcactttc tccagcgcat aacccgtgac aaacagactg
     2161 gcaacctcag caccgtggtg gatatagagg aaaccggcaa atgccatcgc aaccacaacc
     2221 cagaatacgg accagagcgc agcactcttc agcgagattg gcttgtcatc acggtgcata
     2281 aacaggtcaa taaagatggc accgactgaa agcgcaataa aaacaatgac cgtttcagtc
     2341 gggaagccga tgtgtgtgga aaccatattt tacccttagg gatgtggatc agaggccaaa
     2401 ttcagccggg tcgaagccca acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg
     2461 atcgaagtta ccgtcacttt tcgcaacggc aataccaaca cgtaaggcca gctgagcggc
     2521 ctcaggctga tctttcagcg ccaggatgta tttcatggtt tcgcccttgc caatttcaac
     2581 atcgaaatcg aagcttgaaa ccagtttatt gaagaactca atcacctcat tggtatcgaa
     2641 gaccttcagc tcttctgaag agcgcagaaa gcccatcatt ttctgctttt cttccgaact
     2701 tacgccgtca ctcgatacgg caatacgggc acatacagca acggtgccct gcatgaattt
     2761 tttgtttttg aaacggccaa cctggcgggt cagttcgtca cggcctgagt taatggcacc
     2821 tttaactttg tcgaaaaagc tcatacctgt ttcctttgtt gctgtgttat ttagaaccgg
     2881 cgctccagcg aaatccccag ccaaatgccc ggtccatttc gtcctggcct ttgaagtact
     2941 ggttaattcg ctcgacttta attgcgccgt tttcgtttac gagtctggca atggcgcaca
     3001 atgtgcgacg gttctcacct tcggtcaggc gggtctcaat gggcggttga tccggcacat
     3061 gaatggtgac tacaccatca gtcttgtccc agctgggaac accttcataa ataaaggcgt
     3121 aaatcagcac ttcgcggata tgcttccatt cacgtccatt gatatgcagc cactcgccgt
     3181 ctgatacatc acccgtccgg tcatcgccct tgagctgaac atacggttca tcgcgataat
     3241 caccgaatgc attaccgagc gcctgaatga ccgatttata cccgtcctga agctcaacaa
     3301 aggcccccag atccaggtcg attcctttgg agccgaacat acctgcaaag cctgatttgc
     3361 tactgccccg gtgccagttc aggttgatcc ggatttcacc gaagttatcc cgtttggtca
     3421 ggctgattgc aggcttctct tttgtcagcg ataccttgct caggctgatt tttgtctcaa
     3481 ctggcggagg ggttacaacc ggaggagttg gggctgcggg agccggttcg tcggcaatat
     3541 ttacgccgaa atgctctgcc agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc
     3601 ggaatttcca gtcattatta cgacggtaaa attcccccag aattaaggct gcttcctgac
     3661 ggccgctcag ttcgacactg ccgctaacca ggccggtagc cccttgctcc acgtcgatgg
     3721 aaagattacg aagaccggac accgtctgcc caccgtcaca ggtcacggtg aacgcaattt
     3781 tctggacatc aggtttcagc cggttgagcg cgacagtaaa ggttgagtac tggccttcac
     3841 tgaccaggct gacggtacca tcatcgttac gtggctgacc ataaaacacc atgtccgcat
     3901 caccctgcac cttcccgtcg gcatacaggc gaaacgccga agcatccacc tggccgcccg
     3961 aggtgatccg gacccgcaac tcctgggctg gaacgggggc attgcccccc ggcgtcaggt
     4021 tcattagcga ataaccgctg aaacgatatc ggagtgcata tcgtcaattg tgcggccgcg
     4081 acaggcatgg ccaagggcgg taaagtccca gttgccgtta ttacggcgca gcgaagaaat
     4141 aacgatcccg gtatgtgagc cctgctcagt cagcttgtag cgagccagct ctttgccggt
     4201 ttgatcgaca acgcggcaga aagcgttttc gacgtcattg aacgactgac cacggaagct
     4261 gtttaccgta aacgccaggt attcaacgtt tgcaggcagg cgggagaggt tgaccttaat
     4321 cacttcatcg tcaccgtcac cctcaccggt caggttatcg ccactgtgca caacagcgcc
     4381 gcaggtggat ttcagtttgc ggaaccagat ggtgtcaatt gttttacctg tgctgtccat
     4441 taatacacag ccagcatcga gatcgattga gtcgttgcct ccaaacaatc ctccaagaag
     4501 accttttttc ttaaccggat cccaaccgag accgaaatga agctggctta atgcagatga
     4561 ttctttgctg agtgataccg tctggttctt aactaatgaa accatgtttt attccttaaa
     4621 atggtaatga ccgagacaat caaaggtata tttagtaact gacgaagaaa atcatatcat
     4681 gatgaagttc tctgacaagg gaaatttttg tggttttctg taaaaacaca aaaagaaatc
     4741 cttttcaggg tgttagtttt tctattttta atttaaatat caatgtgtta taaggaatcc
     4801 aaaaaacact gacgcactcc ataaccacag gagttattaa tcatattatg attgacatgt
     4861 agcttagaca ctcatagact cacttctcaa tcataaattt gcatggcgtt ccggataatt
     4921 aaaaaaaatg aacaagaaaa tttggtttat ggaaggttta tcctcccagc gagatattat
     4981 tcagggggta aaatcatttg cacaaaaaaa taattttgcc attaccgttt ttgcctccca
     5041 ccgtaacgaa agaaatgaaa tcctttccgt tgccgattat tctttgactg aacctgaaga
     5101 tcctcaaaaa cgtcttcagt ttatccagga aatcattcag acctacggca tccaccatat
     5161 tcatactggc cgtaacagcc agtggtttga agaacaccgt tcagccattg aaccgaccgg
     5221 tgccaccctt actaccggtg caacgggcgt cgactggtta actctggctg acgaaaaagt
     5281 tacttttgct cagtttatgg agcaaaaggg tctcccggtc gtaccatcct ggcgggtgaa
     5341 tacgctggca gaattaaaga cacacctcgc ggccccgccg ttcactgaca gcccggtatg
     5401 cgtgaagccg gtgacgggta tctatggcat gggattctgg cgctttgatg acagtgcttc
     5461 gcctatggcc gtctttaatc atcccgaaca tcgtctggtc agtccgcaac agtatattgc
     5521 agcagcatca gctgctgagt cgtttaaacc ccttgttttg atgccgtacc tgccaggccc
     5581 ggaattttcc gtcgatatcc tcgcggataa gggcgaaata ctcgcagccg tgggacgccg
     5641 taaggaaggg gctatccagt atctggtaaa cgaaggaagc gcctgggaac tggcgtgtga
     5701 ctgcgcccgt gttatgaagg ccgacgggct ggtgaatgtt cagacgcgaa acgatgtgaa
     5761 tggcaacccg gtgctgcttg aaaccaacat gcgtccgtca gggggggtgg gttataccct
     5821 tcacagcggt gtgaaccttc ctgggttatt tgctgccttt aagctcggtc tgatgtctga
     5881 agatatggta cgccagagcg ctaaaaacac cttttctccg gttgcggtga gatccattac
     5941 ggatgtaatt gcatacccgg aatcactctc taaccttctg aattaaggcg tatttatgtc
     6001 tgatatcctc gaagatatga tttatcgccg taccctctca tgcggtacga ttcaggtaac
     6061 ccgtgaccag ggtgaagttt cgctcgatga cctgtttgac atcgctgaaa gacgtaaccc
     6121 gaaacgcgcc tttctgtttg tcagcaaagt actcggcagg cacattcccg tgccaccctc
     6181 agtcatgcgg caggcctaca gacagcttgc cagccagttc ccctcgacac tgacaggacc
     6241 cgtactgttt atcggtatgg ctgaaaccgc cgttgggctt ggggccggtg tatttgatga
     6301 agtgcgccac cagcatcgtg aatctgtcta tctgacctct acccgtcacc cggttgatgg
     6361 cacgttgctt tgcgagttca aggaagaaca cagccacgcc accgatcatc tgatctatct
     6421 gccagatgat gaagagaaaa gacgtcgtgt taccaacgca cgaacgctgg ttttgattga
     6481 tgacgaggca accaccggta atacctttat taacctgctt tctgccctgc gtaataccgg
     6541 caagcttcaa catattgaac aggttatcgc cgtaacgctg accgactgga gtggcaatgc
     6601 cctgtcggag cgcaccccct tgcccgtcac ttcggtttcg cttgtaagcg gtcagtgggg
     6661 atggacccca ttacctgatg cacctgtccc ggacatgccg aaagtcaacg taacttcacg
     6721 aggagaatgg gacatccagg ggaaacagtc atggggccga ctggggatgc tcgcacctgc
     6781 ggccgatctc ggccatgagg tctccgtcca caagggggaa cgtattctgg ttctcgggac
     6841 cggggaattc gtctgggagc cgttcctgct tgctgaacgg ctcgaagctg ccggagcaca
     6901 ggcattttat ggatcgacca cccgctcccc tgtcgccgtt ggttatgcca ttgagtccgc
     6961 catttccttt acggataact acgggctggg catccccaat tttgtctata acgtcgccca
     7021 ccagcagttt gaccgcattc ttgtgtgtac tgagacaccc gcagaaagta ttgacacgca
     7081 gcttcttaaa gcgctggctg aggttgcgcc cgtcgtggag attgttacct atgaataaac
     7141 ccgttgttct gagcgacctt gacgacacgc tctttcagac ccgtcgtaaa atggtggacg
     7201 agctggccct ggagccattc cgtaccggtg ccgttgatcg caccctgaat ccacgaagct
     7261 ttatgacgga agaacagtcc atgttggtgg actggctcct ggagcaggct gaactcatac
     7321 ccgttaccgc acggggaact gaagaaatca gccgcgtacg gatccctttc cactcctggg
     7381 caatcacaac gcacggggcc gtcattctca cgcctgaagg caaacccgac gaggagtgga
     7441 aggcccatat gctcggccag ctggctccct accaggaaaa gctgacatcg atgcagcgtc
     7501 tgatcactga aatgatggac gcaaaaggaa tcaatgcctg ggcaaggctg aacttcgaat
     7561 acggtgaaac ggcggtttat atggtgatga aacaccgcga cagcacccgc cttgacgagc
     7621 tcaatgccat tgcagatgag atagaaacgg tgtttccgac cgagggcttc tacatccacc
     7681 gcaacagtaa taacgtggcc tggcttccca ccccggttga gaaagggctg gcagtcagct
     7741 ggctgcttga aaaacttcgg gctgaacgag gagttttccc cgtaattggt ttgggcgaca
     7801 gcctgagcga tcatcgtttt atgaaactgt gcagctggtt tggcatcccg cgtcagagtc
     7861 agtttgcaga tgccatttca cagcgaattt ttggagaaaa ttaaaatgca gccacatgaa
     7921 acatttaccg gctcatacca gcccggtgac gtggaatttc tgctaaagcc ggttgtcatt
     7981 gagatgacgc cggttgagaa aaaagaagaa ctgattcagt ccgggaagaa acattattcg
     8041 gacatgctca gccaggagcc agcgcctaca caatggcatc ttgatttgtt tcaccgggcg
     8101 ctggatcggg gcgcagagag gcttgcaaaa gaagtcacac agctcgctat tgctctggcc
     8161 gaacgcttcg gtgatgagcc cattgtactg gccagtcttg tcagggctgg cgttccgctc
     8221 ggcgttatgc tgcaccaggc cctgcgtgac atggggaaaa cctcatggca ttacggtatc
     8281 agcattatcc gggatcgtgg aattgacggt gcagccctcg acgtcattga agagcggcat
     8341 ggtaccagcg gtattgtctt tgttgacgga tggacaggta aaggcgcaat taccggagag
     8401 cttgtacgcg cactgaaaga tcgcccgggc tatcctgagc agccgcgact ggttgtcctg
     8461 gccgatccct gtggctgctc ctggcttgca gccagtgatg atgactggct cattcctttt
     8521 ggcatcatgg gggcgccggt atcaggcctg atctcccggt cagtatggtc ctctgaagga
     8581 ttacacgggt gcatggtttg cgagcatctc agtgaattcg aatgcagccg gatgcttgcc
     8641 gataccgtcg ctcatttccg taagaagtta acaccgtcat ccctcgctcc cctgagctgg
     8701 aatacggaat cagcccgggt cttatggcag acaagtcgcg acgttatcgc gttcctggcc
     8761 gatgaattta aagtggacag cgtcaatcgt attaaacccg gtattgctga agcaacccgg
     8821 gctgtattac gtcgggtacc ggaccatgta tttgtgcgtt ctattgacga cccggacgtt
     8881 gccttgcttg tagggcttgc tcgtgaaaag ggaatagttg ttacagaaat ggggggaacc
     8941 ctcggccagt atcgggctgt aaccattatc aagaaggtac tctgatgaaa aatcgtcttt
     9001 ctccctggaa tctgggagcc acgctataca tgcctgcaac acgggaagat attgctgatg
     9061 ccgtcctgca cgggaaaatc ccgggcttgc gctctctggt gatttgcctg gaggatgctg
     9121 tcagtgaagc agacatcccc gtcgccctca aaaatctgga gcacctgctg cacgagctca
     9181 gtaacagcat gagtagcctg ggtaaaaatg actggcctct ggtgttcatt cgcccccggc
     9241 atgccgaaat gggcagatgg ttaaaagcgc attatgatct ttccgctgtc gacgggtttg
     9301 tgctgcctaa atttaccctc agttcgttag ctgaatggtg ggatatcatg gtcgggactc
     9361 acctgtgcat gatgccaacg ctggaaacag aagatgtctt tgacgtggtt cagatgcgcg
     9421 agctggctac ccgtctggag gaacatccct gccacgaccg cattattgcg cttcgaatcg
     9481 gcggcaacga tcttatgaac gttgtgtcgc ttcgccgtcc ccgggacctg acgctgtatg
     9541 acagcccgat gggctacgtc atcaaaatgc tcgtttcggt cttcggcccg cgtgattttg
     9601 ccctgaccgc gcctgtatgt gagcatattg acgatcatgc cgttatggcc agagagctgg
     9661 ctcttgatat ggcacacggg cttgttggga aaacggccat tcacccgggt cagatagagg
     9721 tcattcaaaa cgcgctgatg gtcactcagg gtgagcattc tgacgctctg cggatcctga
     9781 actctaccca ggccgtgttt aagtcgcagg gagcaatgtg tgaacccgcc acacatcgcc
     9841 gctgggcggc tggcattctg gacagggctc gtttttatgg gttacagaac gagcaaagcg
     9901 ctgatggaat cagattactt accgtgaccc agcatcatta aggaaatcat aatggggttc
     9961 aggttcagaa agtcgatcaa cattattcct ggcgttcgcc tcaacctgag taacggtgca
    10021 ccgagcctga gtgtcgggcc gagaggtgct tccgtttctt ttggtagccg ggggacctat
    10081 gccaatctgg gcttgcccgg taccgggctg agttaccgta cccggcttga ccgggccgcg
    10141 cgttccagag gtgaaaaccg gacggcaacc gacccggggc tcagacaggc gcttgagcag
    10201 aaagccgctg aactcatgtc agcggtaacc gcaatccgta atattcacga gctgacgccg
    10261 gatccaaaaa caggcatcag ctgggcagag ctggaagcag tatacctgca taacagaacg
    10321 tcgccttttc aggttccggc accggtgcgt ccagaaaagc cagactacct tgtattgccg
    10381 gaaaagcctg ccgagagcga aggcattagt tttttgggta aatggtttga atcggaatca
    10441 gctaaagctg agcgccacgc cgaaaatctt cgccggtggc agcaggagct gattgatgtg
    10501 gagcgtgaga atacccttcg acagcaccgg taccagcaac aacggacggc ctgggccgaa
    10561 cagtatgcaa actggaagtt tgaagctgaa gaacatgaaa aacggctcgc cacggctcag
    10621 gcagatgccc ggcagcagtt ccggacagac gccgcgtttt tcgaatcata cctggcgggt
    10681 gtgctggcag aaactgaatg gccgcgtgaa acgcttgttg catttgaagt aaagccggag
    10741 ctatcagcag tcctgctgga cgttgattta gctgagattg aagatttccc tgataagatt
    10801 tacggcgtta atgcccgggg aacggagctg acggaaaaag ccatgacgca aaaagccgta
    10861 cgcgaaaact atgcccacca cgtccatggc tgcttgttcc gcctggtcgg tatcgtttta
    10921 catacgctac ctttcgacaa cgtgattgtg tcaggcttta cgcaacgggt cagtaagcgg
    10981 accggctatc tggaggatga gtatatcctg tcctgcaaat gcactcgcag ccagatgtcg
    11041 tcagtaaatt ttgcaggcat aaaacacatt gatccggttg aagcgttagg cgatcaaccg
    11101 gttattcgaa agatgagcag taccttcatt tttcagccta ttgaaccact aaccctttaa
    11161 gcaggctaaa tcttacgatg caattaaata ccagacaggc ccggattttt aagcttgcca
    11221 acttactggg caccggcaag cccgtttccg ctgccgacat tataaccagt ctggaatgct
    11281 ctgagccgac attaacccgg gccctcaaag agctacgcga gtcatattct gcggagatca
    11341 aatacagcaa ggcaggtcat tcctatcatc tcgttaatcc gggccagttg gataaaaaga
    11401 ccctccgccg gatgaatgaa gcgctcgcac agaatgctga actgaaaacc ggtgagtcca
    11461 cagggaaggt cgtactcgat aaggataaaa aaacagccgt gtcattatcg ctacgcatgc
    11521 ggattctaag aaaaattgac cgtctggctg cgctgagtga ctcgacccga agtgaagcgg
    11581 tagagaagct ggctctgcac tccgttgatg aactgataaa agaatacagc gctaaaaagt
    11641 cctgacgcta aatctcagac cggggtgaaa actccggttt tgtcatattt cacctctgta
    11701 ttggcgtgat gcaaaatggc atctgaatcg atcatggttt ggcatccatt tccgatcaca
    11761 gtttggcatc acaatcgatc acgatttggc atgcttccgc tcattgattg gcatcctggt
    11821 gtcgttacgg ggaagtcatc ttcacaggga tcttcagtca gttagcatta ccgacatcca
    11881 ttcaccaact taccggagat aatcaatgtc aaaaccccga tggacgcttg atcagaaaaa
    11941 acatcacgtt gccgcatggc gcgccagcgg ccttacccgt gaacagtact gtgaacttta
    12001 cgacattccg tttaaatccc tgcgccagtg gccgcaggat gtcgcaaaag ccgaaaaacg
    12061 agccagagca ccagaaatta ttcctgtttc agtatctggc tcttccggaa tgactgatgg
    12121 cagaccattg tcggatgagc ctgtcactct cttcctgccc ggtggtatcc ggatgtgctg
    12181 ccagccttct cagttaaccg atgttttccg ggctcttcgc catgctgacg cctgataacg
    12241 tattcctggt cgtaaaaccc gtcgatatgc gccggggtat cgacacactg acacagtacg
    12301 tccagaacga acttaacgct gcatggcatg acggtgctgc ttttgtcttc accaataaag
    12361 tccgctcccg cataaaagtt ctccggtggg acaaacatgg cgtatggctg tgtacccgac
    12421 gactgcaccg gggaagcttc cgctggcccc ggaaaggtga tgccacatgg catctcacac
    12481 aggatgagtt tcactggctg gtctttgggg ttgactggca acaggtgaaa gggcatgacc
    12541 tggcaaaatg ggtataccag gaagaacccg aagatgctca gagcaacgca ataactcaat
    12601 gaaatataat gaatatccat gcgtgatgtc atataatcca ccgcatggat atctcgcttc
    12661 tcagtaccac cagtgaccct gaacagcttc gcgctctggc aattgccatg gtgcaaaaag
    12721 tcatggctga gaatgcagaa cttcagaacc gtatccgcat cctggaagag cagatgaagc
    12781 tcgcacgcca gcagcgtttc ggtaaaaaat gcgaatcgct ggccgggatg cagcgttccc
    12841 tgttcgaaga ggatgttgac gcggatatcg cagaaatctc ggcgcatctg gacaaactgc
    12901 ttcctcagac cggggacgaa gaaaaaacaa caacacgtcc tgttcgtaag cccttgcctt
    12961 cacctcttcc ccgtgctgaa aaggttatcc cgccagctga ggaacgttgt cctgactgtg
    13021 atgcccccct gcacttcatt cgcgatgaag tcagcgagaa gctggaatat attccggcac
    13081 aggtcgtggt caaccgctat atccgcccgc agtacagctg tccctgctgt gagaaagtgt
    13141 tcagcggaaa aatgccggca catatccttc cgaaaagtgc tgttgagcca tcggtcatcg
    13201 cacaggttgt catcagcaaa tataccgacc atctgcccct gtatcgccag cagcacattt
    13261 tctcccgaat gggggtggag ctccccgtca gtacaatggc ggatatggtc ggtgttgctg
    13321 gcgctgcact ggccccgctg gcaaaattgt tacgccacga gcttctgaca cgggatgtta
    13381 ttcatgcaga tgaaacgtcc ctgaggctcc ttgatacccg gaaaggcggt aaatcctgct
    13441 ccggctggct gtgggcttac gtcagcggag aaagagtaag cgtcaacgga gcaccgtatt
    13501 gacgcttatt tattggtgag tactacgttc catggcagga gttcatcaac tcggttggaa
    13561 ggccattctg gcagtacgct caggatatgg cgcagatacg cttccggatc gataccgttc
    13621 agacggcagg tgccgatcag cccgtacaac agtgctccac gctcgccgcc gtgatcgctg
    13681 ccaaagaaca taaagttttt ctttccgaga cagactgcac gaagcgctct ttccgcagca
    13741 ttattatccg cctccgccag accgtcatca ctgtaataac agagggcatc ccactgattc
    13801 agtacatagc tgcacgcttc gcccagtctg gattttttcg acagcgtacc attcttctcc
    13861 accatccatt catgcagtga cgtcagtaac actttgcttc gctgctgcct gacggcaaga
    13921 cgctctgact ccggtaatcc ccgtatttca tcctcgatgg cgtacagttc actgattcgc
    13981 ttcagggctt cttctgccgt cgcacttttg ctgctgatgt atacatcgtg gatttttcgc
    14041 cgggcatggg cccagcacgc aacttctgtc agtgcaccac cttcacgttc tgcactgaac
    14101 agccgatcgt aaccgctgaa cgcatccgcc tgcaggatac cccggaaggg acgaaggtgt
    14161 tgtaccggat gttttccctg cctgtctggt gagtaggcga accagaccgc cggtggctct
    14221 gatgagcccg cattccggtc atcccggaca tacgtccaga tgcgtcctgt ttttgccttt
    14281 tttctgcccg gtgccagcac ttttactggt gtgtcgtcag tgtgaacctt gcgggtgttc
    14341 atcacgtaac ggtacagggt atcattcagc agagtcatta actgacaaca tgcgtcaacc
    14401 cagttggaga gtaatgcccg gctcagttcg atgccctggc gggcaaagat ttcactctga
    14461 cgatacagtg gcaggtgttc gcagtatttt cccgttaaca cgcgggcaag taatccggag
    14521 cccgcgatgc cgcgctctat cgggcgggac ggcgctggcg cttcaactat acagtcacat
    14581 tttgtacagg ctttttttac ccgaacagtg cggatcactt tcagggcgct actcaccagt
    14641 tccagctgct cagcactgac ttcccccaga taatccagct cactgccaca ctccgggcaa
    14701 caactttctt caggctccag acggtgtatt tcacggggaa gatgtgccgg taacggacga
    14761 cgatgacgtg actgtcgcaa ctggcgggga acctgcgggt catcctcacg cccactgtaa
    14821 cgatcgcttt cctgttcgcg ttgtttcagt tgggcctcag cctgttcaac ctcacgctgc
    14881 agtttttcag aacgggtacc gaacaacatc cggcgcagtt tttctatctg ggccctcaga
    14941 tgttctattt cccgctcctc ctcttcgatc ttttcctcgg cacgtgccag tgcagagcgc
    15001 aggaaggcct ccgtctcttc aaccagactc agttgctgat ctttctgacg gagggcttca
    15061 gcctgctcag agagcagcct ttccagctca gtgatacgaa tgaggtattt ccgactcatg
    15121 accgttttta taatccggcc atgacatttt tacaacattg tcagtgcatt aaggcgggat
    15181 gttttgggct gacgccagtc cagtttatcg aggagcattg ccagctgcga gcgggtaatg
    15241 gataccttac cgtcacgcac cgcaggccag ataaactggc cttcctccag acgtttggtg
    15301 aacaggcaca gaccatcagc atcagcccac agaattttaa tcgtgtcacc ccgtcggccg
    15361 cgaaagataa acaggtgacc ggagaagggg ttctcatcca gcacatgttg tacctgttct
    15421 cccagaccgt tgaaggattt acgcatatca gtaacgccgg caaccagcca gattcgagtg
    15481 cctgatggga gcgagatcat cgtcccctcc cggtcagttc acggatcaac accgtgagca
    15541 gctctggtga aggattttcc agcgtcatgt taccgtggcg gaactcaact ttacaggaac
    15601 tggcactgac tgtgctttgt gaaggagtgg ataaaagcgg agtaagagcc gccataggct
    15661 ctttctgctc atcaggcgtt atctcaacag gtaataattc aacgccagcg ccagaagagg
    15721 ttgttaccgg aagacgccgc gatatacgcc tttcgttctg ccagagcctg agccatttga
    15781 acaggaggtt atcattgata tcgtgttccc gggcaatacg ggcaacagag gctcctggtt
    15841 gtgaagccag tttaaccatt tgaagtttaa actcatttga aaatgatctg cggggttctg
    15901 aggataatat tttctgttcc ataacaggtg tccactagtt gaaaaggtgg gcacctacgt
    15961 taccaatact ggcttaatgg ctacatacgg cggtcagttt acgcttacga gaaagaagcg
    16021 gtccgccggt cgtctgtttt gactcgcaga ccggacgggc cctcagatat cctgaggcct
    16081 ggctgcaggg ctggtgtggc ggtacgctgg tgagtgacgg atacagcgtg tacaaatctc
    16141 tggcggataa tgaccccggt attacatctg cctgttgctg gagccacgca aggcgcggtt
    16201 tcgcaaatct gtataaggcc agcagggagc cccgtgcagc catggcgctg agaaaaatcg
    16261 cgggtcttta tcgcattgag aagttcatcc gtgaacgtcc tgtggaaaaa atacggcaat
    16321 ggcgacaacg gtattcccgg ccgatagtga atgacctgtt tgcctggcct gaagagcagg
    16381 agccgtgctg tccgcccgac ggaccactaa acaaagcgat taattatatc ctgaaccggc
    16441 gcgatgaact gagctgcttc ctgagtgacg gagccgtgcc cctggacaac aatatctgcg
    16501 agcgtgctat ccgcccggtg gtcatggggc ggaaagcatg gttattcgcc ggttcactga
    16561 tggccgggaa ccgcgcggca cagataatga gccttctgga aacggcaaaa cgtaatggtc
    16621 tggagccgca tgcctggctg acagacgttc tgacgcgtct accggagtgg ccggaagacc
    16681 gactggaaga gttgctgccc cttgagggat ttaccttctc cgggtgagtg atacctgcca
    16741 tcaggtgttc gtgcagcggg ccataacccg cagtcgggag ttgaacgcct gacgacagga
    16801 aatgagccag agcagcgacc acgcgggctg acagtgagtc agaacctgaa tcagcccgga
    16861 gccattcccg gggctgtact tcatcacctg ccatcagtac ccgtgtcagt acttcgcgac
    16921 ctgacgcaat tacccgactc agttcttcag aacccgcctc cgataccagc ctggcggtcg
    16981 cccctgtgca acaaaataaa tcaaccatcg tcagacggtg atcaatcgta tgcgctgcca
    17041 gtggctcgcc tgaaaaatgc gtcaatatga gttcgccgga acaatacctc agttcttcag
    17101 aacccgcctc cgataccagc ctggcggtcg ccccggcgca acagaataaa tcaaccatcg
    17161 tcagactgcg atcattcgta tgcatcgctg atgatgcgcc cgaaaaacgc gtcaatatga
    17221 gttcgccgga atattactga cgacacggtt tgtagttacg ttccttcggt gagtgagtac
    17281 ctgtgcgtca gggtaacgca atcagttcag gtttagcggg gtgtcaagtc ggtttggcta
    17341 accgaccgca gaattaactg tacctttaaa ggtcgccccc gatatatctg cctgtcagac
    17401 atatcgccga ttcggtatct cactacataa aactccttaa aaaacaatgc gattctgttg
    17461 gtacgaagct ttcagtaagt cagtccggag aagcgccagt acgatacaga atgaggttga
    17521 aaaaaacgtt cacgtctggt cacttcttca aatgttaaaa aacatctcgt aaagagccag
    17581 tttatgcggg ttcgttgagt agacgtgcga gcgtcacttt gtcagcttta acgctgacga
    17641 aagtgacgcc cgcacagctt caaactccac atgcacacgc aagcgaccaa atttcaaatg
    17701 gtcttatgcc gtgcgcacag tcataagaag cgcatgcgga aagtcagaaa atatcttaag
    17761 acgcagattt taaaggcgtt tttatgccag tctgccggga atacacagta gtccggttgt
    17821 ccggaagcac cacggtaatc cgaaggaaaa atcaacgtac ctcaatcacc gtagtgatat
    17881 ccgttcccag aggctaaacg gatgtaccac cataaaggca tcctctttca ggctacgagg
    17941 atattgcaat cacccgaagg cgtttctctc agattgctga aacattggct ggcagtcacc
    18001 cgaaggcgtc cctctcagac tgctggaaca ttggctggtc gcaaaagcga cagttaatgc
    18061 aaatacaggt taacaaaaag ggaaatctgc cgtcaaccca accatatgaa catgttgatt
    18121 tcattatgaa tgtgatgttc taatataaat taatcagtaa cttcataaaa agaatataag
    18181 aaacagataa tgcatgtatt tacacacata tcagcacaat gatagattac caccccagaa
    18241 acacgataaa ggtataatta tggaattttc cgttatttt
//
LOCUS       DAZFYC010000098        18084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFYC010000098 DAZFYC010000000
VERSION     DAZFYC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.098"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(273..1865)
                     /gene="lsrK"
                     /locus_tag="ACHKW5_003687"
     CDS             complement(273..1865)
                     /gene="lsrK"
                     /locus_tag="ACHKW5_003687"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO7513008.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1944..2897)
                     /gene="lsrR"
                     /locus_tag="ACHKW5_003688"
     CDS             complement(1944..2897)
                     /gene="lsrR"
                     /locus_tag="ACHKW5_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO7513009.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3146..4681
                     /gene="lsrA"
                     /locus_tag="ACHKW5_003689"
     CDS             3146..4681
                     /gene="lsrA"
                     /locus_tag="ACHKW5_003689"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO7513010.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4675..5703
                     /gene="lsrC"
                     /locus_tag="ACHKW5_003690"
     CDS             4675..5703
                     /gene="lsrC"
                     /locus_tag="ACHKW5_003690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO7513011.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5703..6695
                     /gene="lsrD"
                     /locus_tag="ACHKW5_003691"
     CDS             5703..6695
                     /gene="lsrD"
                     /locus_tag="ACHKW5_003691"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO7513012.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6707..7729
                     /gene="lsrB"
                     /locus_tag="ACHKW5_003692"
     CDS             6707..7729
                     /gene="lsrB"
                     /locus_tag="ACHKW5_003692"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO7513013.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7756..8631
                     /gene="lsrF"
                     /locus_tag="ACHKW5_003693"
     CDS             7756..8631
                     /gene="lsrF"
                     /locus_tag="ACHKW5_003693"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO7513014.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8655..8945
                     /gene="lsrG"
                     /locus_tag="ACHKW5_003694"
     CDS             8655..8945
                     /gene="lsrG"
                     /locus_tag="ACHKW5_003694"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO7513015.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9002..9760
                     /gene="tam"
                     /locus_tag="ACHKW5_003695"
     CDS             9002..9760
                     /gene="tam"
                     /locus_tag="ACHKW5_003695"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO7513016.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9764..10678)
                     /gene="yneE"
                     /locus_tag="ACHKW5_003696"
     CDS             complement(9764..10678)
                     /gene="yneE"
                     /locus_tag="ACHKW5_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO7513017.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLHFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10885..12336)
                     /gene="uxaB"
                     /locus_tag="ACHKW5_003697"
     CDS             complement(10885..12336)
                     /gene="uxaB"
                     /locus_tag="ACHKW5_003697"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO7513018.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12563..13981)
                     /locus_tag="ACHKW5_003698"
     CDS             complement(12563..13981)
                     /locus_tag="ACHKW5_003698"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7513019.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14120..14479)
                     /gene="yneG"
                     /locus_tag="ACHKW5_003699"
     CDS             complement(14120..14479)
                     /gene="yneG"
                     /locus_tag="ACHKW5_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO7513020.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14479..15405)
                     /gene="glsB"
                     /locus_tag="ACHKW5_003700"
     CDS             complement(14479..15405)
                     /gene="glsB"
                     /locus_tag="ACHKW5_003700"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO7513021.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15469..16857)
                     /gene="sad"
                     /locus_tag="ACHKW5_003701"
     CDS             complement(15469..16857)
                     /gene="sad"
                     /locus_tag="ACHKW5_003701"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO7513022.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16958..17839
                     /gene="yneJ"
                     /locus_tag="ACHKW5_003702"
     CDS             16958..17839
                     /gene="yneJ"
                     /locus_tag="ACHKW5_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7513023.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTLPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            17916..>18084
                     /gene="yneK"
                     /locus_tag="ACHKW5_003703"
     CDS             17916..>18084
                     /gene="yneK"
                     /locus_tag="ACHKW5_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO7513024.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSAT"
BASE COUNT         4549 a         4624 c         4502 g         4409 t
ORIGIN      
        1 tatttaacaa agaatagcac taattgctaa aaatcgaagt ttattaaacc ccttttgcat
       61 ccattaatgt ctttaggaat attcgctata aaataagggt tatgcttaat gctttaagaa
      121 aaatagcaat tttcccttga atatagtact ggtgatggaa cgatgaatct gcaagctggc
      181 atttaacaag ccagctctaa aagaagggaa ataagaaaaa ctatactcaa aaactaacag
      241 ccacggtcat catgatgtgg ctgtcaatga aactataacc caggcgcttt ccataacgac
      301 gtcgtcagtc catgatcaac cagccccagc tgatcctgat aaactgcctg ccacttatcg
      361 cgtgaatcct gataaagttc atgcttttcc gggtctggtg tgtgcgtccg ttcccagcga
      421 accaggcgtt ctccggtttc tgccattgat gaaaaaattc cggcaccgac gccagctgca
      481 atggcacatc ctaatgcggt ggcttctttg actaccggaa tattgacggg taatcccgag
      541 acatcagcga gaatttgact ccataatttc ccttttgaac ctccgccagc aaaaaccagc
      601 gaggtgggat gaatattcga gaaatcagca atttgctgca agttacacgc tgatacaatc
      661 gccgcatttt cttccagcgc acggaacaat gtcgctttgt tacatttatc cgggtcaatg
      721 gacaagttaa taaaggaagg cgcagcgtga taccaggttt taaagcgcat tctgtcggag
      781 aagatcggca ttacgcccca cgacccaggc ggcacccgac tggccatctc ttccagcagc
      841 gtataggtgt cgatgcctaa acgttcggca atcagttttt cttcggcaca gaaagcatcg
      901 cggaaccagc gcatggtgag tccggtaaaa aagcttatag attcagcttg taccatgcca
      961 ggaataacat gaggattaac gcgcacgttc atttctgggt ctgtcaccgg cgcggctaaa
     1021 tttacaactt gctgccagaa tgtgccgcca agaaccgcgg tttgtgccgg acgcacaacg
     1081 cctaacccaa ggcaaccaag ctgcacgtcg cctcctccaa cgaccaccgg agtgcccgcc
     1141 ttcagaccgc agagttccgc cgcttgtgaa cttaccacgc ccagcaatgt gccggtttct
     1201 ttgacaggag aaagaatatc ggcacgcagg ccagccatat ccagcaatgc aggtttccag
     1261 tcacgggtgg ttagatcaag aagtcccgtg gtgccagcgt tagagggatc caccgccagt
     1321 tcgccgctga gcatataggc cagccagtcg ctgatcatgg tgatggttga tgcctgacgg
     1381 taaatatcgg aacgatggtg cgccagccaa agtaatctgg ggatggcact taaagccagt
     1441 gtttgtccgg tcgcgcgata aacttcgttt tcaaaggtat tgttgtgcag ttctttaagt
     1501 tcgctaactt cgcgtgccgc tctggcatcc acattggcgc aggcccagat cggggctcct
     1561 tcattattat ataaaacaat gccttcacgc atcgaacatg ccgaaacggc agcgatatac
     1621 tccggggcta tgccggcgtt gtgcagcgcc tggcgcatac actcacacgc cagttgccag
     1681 tttttgttga gatcaaattc catagaacca ggaacgtccg gtactgccag gtgccgccac
     1741 tccgcctgtc ccactgctat ttgattgcct tccaggtcga atatcacagc ccgaatactt
     1801 ccggtacctg catccagcgc catcaggtaa tactttgatt ctgaaggggt aaagagtcga
     1861 gccattagat tatcctcggc ttatgcgttc tgtctgttcc tctatacgtt ctccatcatt
     1921 cccggtaata aggtcatgca aatttaacta cgtaaaatcg ccgctgctgt gtcctgatcg
     1981 gtaaccagtg cgttgatata accgcctttc attgcagcgg caattgcttc ggctttattt
     2041 tctccccctg ccacgccaac ccggacgggt atggtcttca gcgagcttaa aggtaagcca
     2101 atcagttcgt tatgtatttt aatatccgtg acaacgtcac cttttgcatc aaaaaagtag
     2161 cctaaaatgt cgccaaccgc cccttttcga ccaatcatta actgttcgcc ctggctgata
     2221 taaccggagc gaatgatcgt cgcgtcgtcc tgttgactga cggccccaat gccgacaatc
     2281 gccacatcgg ctgcttgcgc ggctaacaga acatctttga cgcaattttc attttttagc
     2341 gtacgggcaa tgtcagcgga ggatgcccgc aacggagccg gaataatatt cacactgcac
     2401 gccgcgttaa gctgcccgat acccgtcata taagaaccga cgccaccgga gagcgtgacc
     2461 aggcgaattt gctgtgacga aataaaacca cttaagcgtt gcagcgtatt catggttgcc
     2521 tcgccaaaac caatcgccag catctgttgt ggttgaagta aactcatcaa catatgcgcc
     2581 gcgcctatcc ccagtcgccc gccgacatca gcatccgcaa gcccagggat cacccggaca
     2641 tgttgcagcg aaaactgacg acgtaattga gtttcatatt ccagacagcc ttcaaagcga
     2701 gaattaatct gtacgcgaat aatgccggac tgatgccctt tctccagcaa gcgcgacact
     2761 ttcaaacggg tcaggccgag acgatcgctg atctcgctct gggtcagccc gtcgtgatag
     2821 taaaaccacg cgatccgcgc gacctgttct tcttcacaca ttccctgttc tgaaattgcc
     2881 gaatcgttga ttgtcataat tcattcttca ctttgaacat atttaaatct ttaatgcaat
     2941 tgttcagttc ttgcgcattt atatctgtga tggcagccac agtttgactc tacgagcatg
     3001 aacaaacgca accgtgaaaa tcaaaatagc ataaattgtg atctattcgt cggaaatatg
     3061 tgcaatgtcc acctaaggtt atgaacaaat taaaagcaga aatacatttg ttcaaaactc
     3121 acctgcaaaa ctgaacgggg gaaatatgca aacgagtgat acccgcgcgt taccgctact
     3181 ttgcgcccgc tcggtttata aacagtattc aggggtcaat gtcctgaaag gcatcgattt
     3241 tacgttgcat cagggggagg tccacgccct gctcggcggc aatggtgccg gtaaatcgac
     3301 gttaatgaag attattgccg gtattacccc tgctgatagc ggtacgctgg agattggggg
     3361 caacaactac gccagattaa cgccagttca tgctcatcag ctgggcattt atctcgttcc
     3421 ccaggaaccg ctgcttttcc caagcctgtc gataaaagaa aacatcctgt ttgggatggc
     3481 aaaaaaacag ctctccatgc aaaaaatgaa gaacttgctg gcggcgctgg gctgccagtt
     3541 tgatctgcat agtctggcag gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct
     3601 ccgcgggcta atgcgcgact cgcggattct gatcctcgat gaacctaccg cctcgcttac
     3661 ccctgcggaa accgaacgct tgtttactcg cttgcaagag ctgcttgcta ctggcgtggg
     3721 tattgttttt atctcgcata agctgccgga aattcgccag attgccgatc gaattagcgt
     3781 gatgcgcgac ggaaccatcg ccttaagcgg caaaaccagc gaactgtcta ccgacgacat
     3841 tattcaggcc atcaccccag cggtacggga aaaatcgctc tctgccagcc aaaaattatg
     3901 gctggagtta cctggtaacc gcccacaaca tgctgccgga acgccggtgc tgacactgga
     3961 aaacctgacc ggcgaaggtt tcaggaatgt cagcctgacg ctcaatgccg gagaaattct
     4021 gggcctggct gggctggtgg gggccggacg cacagaactg gccgagacgc tctatggtct
     4081 gcgtactttg cgtggcggac gcattatgct gaatggtaaa gagatcaata aattatccac
     4141 tggagaacgt ttactgcgcg gtctggttta tctgccggaa gatcgccagt catccggact
     4201 gaatctcgat gcttcgctgg cctggaacgt ctgcgccctt actcataacc ttcgtggatt
     4261 ctgggcgaaa accgcgaaag ataatgccac cctggaacgt tatcgtcggg cgctgaatat
     4321 taaatttaac caaccggaac aagctgcacg gacattatcc ggtggcaacc agcaaaaaat
     4381 cctcattgcc aaatgcctgg aagcttcgcc gcaagtattg attgtcgatg agccgacgcg
     4441 cggcgtggat gtctcggccc gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca
     4501 aaatgtggca gtgctgctta tctcctccga tctggaagag atcgaactga tggcagatcg
     4561 cgtgtatgtg atgcatcagg gcgaaattgc ccactctgca ctgaccgggc gcgatattaa
     4621 tgtcgagact attatgcgcg ttgccttcgg cgatagtcag cgtcaggagg tgtcatgctg
     4681 aagtttattc agaacaaccg tgaaatcacg gcactgctgg cggtggtgct gctgtttgca
     4741 ttacccggtt ttctcgaccg ccagtattta agtgtgcaaa cgctgaccat ggtttatagc
     4801 agcgcgcaaa tcctgatcct gctggcaatg ggcgcgacgc tggtaatgct tacgcgcaat
     4861 attgatgttt cagtgggttc gattaccgga atgtgcgcag tgctgttggg gatgttactg
     4921 aacgcggggt attcactacc tgttgcttgt gtcgcgactt tactgcttgg tttgctcgcg
     4981 ggatttttca acggtgtcct ggtcgcgtgg ctaaaaatcc ctgccattgt tgccaccctt
     5041 ggcacgttag ggttgtacag aggcatcatg ttgctgtgga ctggcggtaa atggattgaa
     5101 gggttacccg ccgaactgaa acagctctcc gcaccgctgc tgtttggcgt ttccgcgatt
     5161 ggttggttga cgataattct ggtggcattt atggcctggc tgctggcaaa gacggcgttt
     5221 ggacgcagtt tttatgccac gggcgataat ttacagggcg ctcgtcaact gggtgttcgt
     5281 acggaagcca ttcgcattgt ggcattttcg ttgaacggct gcatggcggc actggcggga
     5341 attgtgtttg cttcgcagat tggttttatc cccaaccaga ccggtaccgg gctggagatg
     5401 aaagcaattg cagcctgcgt gctgggcggc attagtttgc tcggcggttc cggtgcgatc
     5461 atcggtgcgg tactcggcgc atggttcctg acgcagatcg atagcgtact ggtgctgttg
     5521 cgcattccgg catggtggaa cgattttatc gcgggcctcg tgctgctggc ggtgctggtg
     5581 tttgatggac gcctgcgttg tgcgctggaa cgtaatctac ggcggcaaaa atatgcccgc
     5641 tttatgacgc caccgccatc cgttaaaccc gcttcgtcag gtaaaaaacg ggaggccgca
     5701 taatgcgtat tcgctacggt tgggaactgg ctcttgccgc actgctcgtt attgagattg
     5761 tcgcatttgg tgcaattaac ccgcgaatgt tagatctcaa tatgttgctg ttcagcacca
     5821 gtgactttat ctgcattggc attgtcgccc taccgctgac gatggtgatt gtcagtggcg
     5881 ggatcgatat ttcgtttggt tcgaccatcg gcctctgcgc cattgcattg ggcgtactgt
     5941 ttcaaagtgg tgtgccgatg ccgctggcga ttctcctgac cttactgctc ggcgcattgt
     6001 gcgggctgat caacgccgga ttaattatct ataccaaagt taacccgctg gtgattacgc
     6061 ttggcacgct gtatctgttt gccggaagcg ccctactgct ttccgggatg gccggagcga
     6121 cggggtacga aggtattggt ggattcccga tggcgtttac agatttcgct aacctggatg
     6181 tgctgggact ccccgttccg ctgattatct tcctgatatg tctcctcgtt ttctggctct
     6241 ggctgcataa aacccatgcc ggacgtaatg tgtttttgat tgggcaaagc ccgcgcgtgg
     6301 cgctttatag cgcgattcca gttaaccgca ccttatgtgc gctctatgcc atgacggggc
     6361 tggcgtctgc ggtcgccgct gtgctgctgg tgtcgtattt tggttcagca cgttccgatc
     6421 tcggtgcgtc gtttctgatg cccgccatca ccgccgtggt gcttggcggt gccaatattt
     6481 atggtggttc cggttccatt atcggcaccg ccattgcggt tttattagtg ggatatttgc
     6541 aacaaggttt gcaaatggca ggagtgccaa atcaggtgtc cagcgccctt tccggtgcgc
     6601 tacttatcgt cgtggtcgta ggtcgttccg ttagcctgca tcgccagcaa attaaagagt
     6661 ggctggcgcg tcgggccaat aacccattgc cataaaggat atcttcatga cacttcatcg
     6721 ctttaagaaa atcgccttac ttagcgctct tggcattgcc gcaatctcta tgaatgtgca
     6781 ggccgcagag cgtattgcat ttattcccaa actggttggc gtgggatttt ttaccagcgg
     6841 tggcaacggc gcacaacaag cgggtaaaga gctgagcgtt gatgtgacct acgacgggcc
     6901 gacagaaccc agtgtttctg gtcaggtaca gttgattaat aacttcgtca atcaaggtta
     6961 taacgccatt atcgtttctg cggtttcgcc tgatggcttg tgtccggcac tgaaacgcgc
     7021 catgcaacgt ggtgtgagag tgctgacctg ggactctgat actaaaccgg agtgccgctc
     7081 ttactacatt aatcagggaa cgcccgccca gttgggaggt atgttggtgg atatggcggc
     7141 gcgtcaggtg aataaagaca aagccaaagt cgcgtttttc tactcaagcc ccaccgttac
     7201 ggaccaaaac cagtgggtga aagaagcgaa agcgaaaatc gccaaagagc atcctggctg
     7261 ggaaattgtc actacgcagt ttggctataa cgatgccact aaatcattac aaaccgcaga
     7321 aggaatatta aaagcgtata gcgatctcga cgccattatc gcccccgacg ccaacgccct
     7381 gcccgctgcc gcacaagccg cagaaaacct gaaaaatgac aaagttgcga ttgtcggatt
     7441 cagtacgcca aacgtgatgc gtccatatgt ggaacgcggc acggtgaaag aatttggcct
     7501 gtgggatgtg gttcagcaag gcaaaatttc agtgtatgtc gcggatgcat tattgaaaaa
     7561 aggatcaatg aaaacgggcg acaagctgga tatccagggc gtaggtcagg tggaagtctc
     7621 gccaaacagc gttcagggct atgactacga agcggatggt aatggcatcg tactgttacc
     7681 ggagcgcgtg atattcaaca aagagaatat cggcaaatac gatttctgat gtgcattact
     7741 taaccggagt aagttatggc agatttagac gatattaaag atggtaaaga ttttcgtacc
     7801 gatcaaccgc aacaaaatat cccttttatc ctgaaaggtt gcggtgcgct ggattgggga
     7861 atgcagtcac gcttatcgcg gatatttaat ccgaaaacgg gtaaaaccgt gatgctggcc
     7921 tttgaccatg gttattttca ggggccgact accggacttg aacgcattga tataaatatc
     7981 gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt gcgcagcgta
     8041 gttcccccag cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa ctctattctg
     8101 gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg cctgaacagt
     8161 tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc gatcaaaaat
     8221 attattcagc tggttgatgc cggaatgaaa gtgggaatgc cgaccatggc cgtgactggc
     8281 gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg aatcgccgct
     8341 gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga acggattgtt
     8401 gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga gcgcgaggcg
     8461 ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat ggggcgtaat
     8521 attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt ggttcaccat
     8581 aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta actgcggatt
     8641 taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa gacaagattg
     8701 acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa gaaggcaatt
     8761 tgcgcttcga tgtcttacag gacccagaag tgaattcgcg cttttatatc tacgaagcct
     8821 ataaagatga agacgcagtg gcattccata aaaccacgcc ccactacaaa acctgtgtcg
     8881 cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat gggttgatgc
     8941 cgtgaggcga attcatcagt tttatctaca attggggtaa cgcgctgacg ggagtaaata
     9001 aatgtctgac tggaacccct ctttatatct gcactttgcc gctgaacgat cgcgtccggc
     9061 ggtggagctg cttgccagag taccgctgga gaatatcgaa tacgttgccg atcttggctg
     9121 tggcccaggt aacagcaccg cccttctaaa ccaacgttgg cctgctgcca ggataacagg
     9181 catcgactcg tctccggcga tgattgctga agcgcgcagt gctttgccag actgccagtt
     9241 cgtggaagca gacatccgca actggcaacc ggaacaggca ctcgatctga tttttgctaa
     9301 tgcctcactg caatggctgc ccgaccacta cgaattgttt cctcatctgg tttctttact
     9361 taatccgcag ggcgtgctgg cagtacagat gccagataac tggctggagc cgacccatgt
     9421 gctcatgcgc gaagttgcct gggaacaaaa ctacccggat cgcgggcgtg agccgttggc
     9481 tggcgttcat gcttactacg atattttgag cgaagccgga tgtgaggtcg atatctggcg
     9541 aaccacctac tatcaccaga tgccgtcaca ccaggcgatt atcgattggg tgactgccac
     9601 tggattacgt ccgtggttac aggatctgac cgagagcgaa cagcagcttt ttcttaagcg
     9661 ctaccatcag atgctggaag agcagtatcc actgcaagag aacggacaga tactgctggc
     9721 atttccgcgt ctttttattg ttgcccggcg tatggagtaa atatcacgtc agctggtaat
     9781 gacgatcggg aagaattttt gctggaattt cggcttcatc gttcatctgt aacaggtcaa
     9841 tttcaatagc gttgcagatg gcatccagtg gtaaatcatt gttttcagta ccgaacggat
     9901 cttccagttc ttccgccaga caatccagcg aaataaaagt gtaggaaatc agcacagaga
     9961 taaaaggcgt catgtaatgc aggtccacga ccagcgcgaa cggcagcatg atacaaaaca
    10021 gataaacggt acgatgcaaa atcagggtgt aggcaaaggg aattggcgta taggcaatgc
    10081 gctcgcatcc tgccaggacc gctgaaatat cattaagccg atcgttgagg ctaataaaca
    10141 ggatatctga aagctgccca ttgcggcgct gaaccgccaa ccactccccc attattaaca
    10201 agatacggtt agctggagag ttcgaagcca gtacaggctg aagatcttca gtcttgagat
    10261 aatgagccag cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga
    10321 aggcgatttg caaccgggca aactccctta cacttgccga atccggcaat gtcgttttta
    10381 cctcgcgcag taacgaccgt gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa
    10441 cgtaacgggc gtacccggca ttattacgaa aaccaagaaa aatggcgatg gcgacaccga
    10501 gaatgctgaa cggtgcgagg gtgaatttga tgcccagatg cgtgtaccag ggcagcatga
    10561 aaataacagc gatagaaaag agaaaatgga gtagtaagcg cgatgatatc ttggataata
    10621 ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg
    10681 ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca ttctcaagac
    10741 gctatttaca aattacctac aagcttacaa cattaacagg cgctgcatgt ggcatctgtt
    10801 aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac ccggtcgaaa
    10861 ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc gcatcccttt
    10921 ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca cttgcgtcag
    10981 gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct gggtaccgat
    11041 aacacggtca cgatgctggc tccagagttg ctgataacgt tccagccagt gtgcatcgtc
    11101 ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca atgccgctaa
    11161 tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca gcagctgcgg
    11221 caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca gctgatgctt
    11281 aatgtacggg ttacggaaac gcccggtgac tgcactggcg aaagattcca gttcatcacg
    11341 aggcaaatcc agtaccggaa taatttcttc gtagatagct ttttcaacga atgcgcaaat
    11401 ttcagcatcg ttcatcgctt cacctacggt atccagccct gcctggaacg ccaccggcac
    11461 cagcgcggtg tgcgcaccgt tgaggattgc cactttgcgt tctttgtacg gtttaatatc
    11521 gtcaacaatc agcacgttga gcggatattt gtccagacgc agttcagtcg ctaaggattt
    11581 cggtccctga atcacaaaca ggtaaaagtg ttcagcggtg tcgagaaaac catcgtgata
    11641 acccagctct tcttccagtt tagccacttc atcgcgcgga taaccggtaa cgatacggtc
    11701 caccagcgta gaacagaagc tgttagcctg atccagccat tgaataaatg cttctggcag
    11761 tgcccactct tgcgcatagc gcagcaccag ttcacgcaac gcgtcgccat tgtagtcgat
    11821 caactcacac gggatgatga tccaaccttt atccagcgca ccgttgaaat ggctaaaacg
    11881 ttcgaacagc agacgggtca gttttgccgg ataacttacc gctggcgcgt catcgaattt
    11941 atcgcccgca tggtagctga tacccgcttc ggtggtatta gagaaaacaa agcgcatttc
    12001 cgggttgtga gccagtttca ggaattcatc gtattcactg tagacgctga tttcacgatt
    12061 aaccgagcga atcagacgcg cgtcgctgac cgcttccccc ttctcattca ggccacggat
    12121 aatggtggta tacagaccat cctgcgtgct cagtgacggt gggaatgaag tttcaatcgg
    12181 acgaacaacg accacaccag aattcagatc ggtgtgctca ttcaggagat cgatttgcca
    12241 gtcaacaaag gcgcgcagga agttaccttc accaaactga atgatacgtt ctggatactg
    12301 tgcaccggga aaatcgcgac gatttagtgt tttcacaatg ggttcccttc tgattagtca
    12361 tacaacctgt ttgaattggt acgacaggtt agcaaacttt aatacgccga acccctgttt
    12421 tgatcaactc ctgatgatta atgagcagtt ttatgagaaa agtgtggcgc ggatcatggt
    12481 ttaatcgagg aaatacgcct tttcctggat cataaagtgg tagaacacat tgcattcaaa
    12541 tcgcgcggaa tgaataaaga tgtcagacaa cttcctcacc gtaacgcata gtgctggtac
    12601 ggttgcgccc atctttcttc gagcgataca gacacgtatc ggcttcgacc atcaatttat
    12661 taaaatcatc ggtcagcgtt cggtacgatg cgcgaccact accgacgcca atacttaccg
    12721 tgagataaag cgttttttgt tgccaggtga atggttgcag ttcaacgcct ttacggattt
    12781 tttccgccat tagcagacca tcgacaggat tcaccgacgg cactgcaaca gcaaattctt
    12841 cgccgcccat gcgcgccacc agccccttat caccgacaat cttctgaata tgccgggcaa
    12901 acacgcttaa cactttatcg ccacattcat gtccatagtt atcgttaatg cttttgaaat
    12961 agtcaatatc aagcagcatg accgtcagat gttgtgtctg tttcagcgat ggacttttca
    13021 gcgcctcata aagaccggac cgtgagtaaa cctgagtcag aaaatcaaag tcggctcgca
    13081 gcgcaacttg cttcattagc gaattgatcg ctgccacgct aaaagaaacc ataattgggc
    13141 atatcgccat cgtggcaata ccgagacgtg cggagaacat ttccggaatg gagaacggcg
    13201 aaccgaccga gatatcaatc accgaatttg ccaccagtac gatttccacc gcaccggtga
    13261 caaaggtcaa cagacatgtt acctgcggcg tatagcgcac tgcacaccag attaatgcag
    13321 gcagcgggaa cgccagactc cccgctccgc caatgactac cgaggcaata accgacacaa
    13381 taagcgcgat ggcgggcatc atctgttctg ctttaaagcg cggcagcact ccaggaatag
    13441 ccaacgtcag catacaaggc acgatcaaca cgcccgttga gaattgctca ctgaaccagt
    13501 ccgcaagcaa aggccagaaa tccagactat caatactgac cgaaccaatc gccccgacaa
    13561 tagcgcataa taatgcggca atcagacagt aattaaatag ccgtaaggca cttactggct
    13621 catacttatt tttccccaga cgcttatccc gcacgaccag taaggcgacg gtaacaataa
    13681 acatcatatt ggagaaattg ataaccagtg aaaccagtcc ccattcggtg gttatggcat
    13741 cataaaccag catcgccaca taactgatcg catagtaatg cagtcgatta agccagacat
    13801 agcgggcaaa cacccccgcc attacgccat tcagcggcca gaaaagagaa agttcgtgaa
    13861 ccagccgtaa ctctgcacca ataaaataga acagcgtggt tagcgcaaaa atagcgatgc
    13921 tattacgtag cagatgacct tcctggaaca aacggaaagt tgaaatgggg tgtacatgca
    13981 ttaattatct gtccatacaa ttgcgggtgg caattatctc aacgtaataa gtaaattcac
    14041 ataaaggata gcatgttcgc taacagcggc ggcaaataaa ggtaccctgg gaatgctatt
    14101 ttatcgtttt ccgcaattct catggctgat tcatctgtac aaccagccag tgatagatta
    14161 ccgccacgat ataacgttgt tgttcttcgc ttaacgaaac accctgcgga atgttatgcc
    14221 acttagccag acaaccgcga cagcaggtcg cggtggcgtg ctgagcgata aacaccggat
    14281 gcccgcgcat gggggtttgc ttaccgtcat tagccggtaa tgccggagca agacgtttag
    14341 cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc tttctccagg caatactgac
    14401 gctctttcat gcccagacga aagcgagagc gaaattttga acgggataaa cgcgcgaaga
    14461 gtggatcgag agactgcatt aataaaccga acgccctaac tgtttcgtca attgttcaag
    14521 gacggcgata cccgcaagcg agttacctgc atcatccagt tccggactcc agactgcgat
    14581 ggccatttca tgcggaacaa tcgccacaat accgccacca acgccagatt tcgccggtag
    14641 tcccacccgc caggcaaact cccccgcgtt ctggtacata ccactggtcg ccatcagcgc
    14701 gttaatttgc cgcgcctgca ttggcgtcac cacaggttca tcaatatgaa tagctttccc
    14761 ctgattagcc agaaagacaa acgtccgggc cagctctaca cagctcattt tcagagcgca
    14821 gtaatgaaag tagttttgca gaacggttgt cacgtcatga tggaaattgc caaacgactt
    14881 catcagccag gcgatagccg catttcgcgc ggaatgttca aattcggaac gcgctaccac
    14941 cgtatcgtat gaaatatcag acacaccgct taagccgcgc acgacttcca gcatacgttg
    15001 ccgtggtgcg cttagtcgcc cttgcaacat atcgcagacc accagcgcac cggcattaat
    15061 gaacggatta cgcggaatac cctgttccat ttccagttgc actaaagaat tgaacggtga
    15121 tccagacgga tctttgccga cgcgttgcca gatttcctct tcggagtaat gacgcatggc
    15181 gacaacgaga ctcagcactt tggaaataga ctgaatggaa aaacgttctt gcgcgtctcc
    15241 ggcctgaaaa agctgtccgt caacggtaca gatagcaatc cccaatcggg aaccgtctac
    15301 tgtagccagc gccggaatat aatccgcgac tttaccctga ccaatgagcg gccgcacttg
    15361 ccgcaagatg ttttctaaaa ttgcattatc catggcgact gccactttct actcctggac
    15421 cgcaggtctg aaaagacctg cgagtatatc agagctgaat atgtcgcgtc agatccggtc
    15481 tttccacacc gtctggatat tacagaattc gtgtaagccg aaatgggaaa gctcacgacc
    15541 aaagccactc tttttcacgc caccaaaggc cactcgcgcg tcgctggcac aataaccatt
    15601 gataaacacc ccaccgcatt ccagacgtgc cgccatctgt ctggcctgtg tttcgtcggt
    15661 ggtaaaaatg gtcgctgaaa ggccgaactc actatcatta gccagttcca gcgcatgttc
    15721 tgcatctttc gcaacggtga ttgccgcaac agggccaaac atttcttccc gaaacgcggt
    15781 catttctggg gtaacattcg ccagaaccgt tggcggatag tagttacctg ccccagccat
    15841 cttttccccg cccagtaaca aacgcgcacc ctgcgccagg gttttctcca cctgatgatg
    15901 cagctcatca cgtaaatcaa aacgagccat tggtccgaga gcgttctctt catcacgggg
    15961 atcgcccatt ttcaaggctg ccgcagctgc cacaaaacgt tcggtaaatg cagaagcaat
    16021 tccctcttcg ataataaagc gttttgccgc tgcacaaacc tgtccggtat tctgataacg
    16081 tccggctacc gccgctttaa ccgccagttc cagatcggca tcgttaagca caataaatgg
    16141 atccgaaccg cccagttcca gtacgcattt tttcagtgcc gctccagcct gtgcgccaat
    16201 agccgctccc gcacgaacac ttccggtcac cgtgacagca gcaatgcgcg aatctttaat
    16261 catttgactg acaccgtcgt tgtcggcatt cagccagcca tatacgcctt gcgggattcc
    16321 cgcatcttta aacacctggg caatgagctg agcacagccc atcacattcg gcgcatgttt
    16381 aagtaagtag ccgttacctg caagaatgat gggaaccgcg ccacgcatca cctgccataa
    16441 cggaaagttc cacggcataa tcgccagaat cgtccccaac ggtcgatact caataactgc
    16501 ctgctgattt tccaccagcg taggttccgc cttcagcatt gccggaccat gttctgcata
    16561 ccagtcacac aaattcgccg atttcgccac ttcagcgcgc gcctgattga ttggtttgcc
    16621 catttcacgg gtgatcattt gcgccatttc ttcgctacgg gcgcgcagag ccttaccgat
    16681 accacgcagt ttttcagcac gataatctat atttgtctcg cgccagtcgc gaaagcctgc
    16741 tgccgccagc tgaagtgcgt tttcgatatc gttagcgcca gcccacggca gcacagaaag
    16801 ttgttcaccc gtggcaggat ttattgaaat tgcatgagtt gccggagtaa tggtcatcgg
    16861 ggtatctcct ttatgagtca tggtatgaag atacgcagat ttactcttgc tttaaaatga
    16921 ataatattaa gccacttatt cacgaatcga gaatattatg gatctgacac aactggagat
    16981 gttcaacgcg gttgccgaag ctggcagcat aacccaggca gcagcaatag tgcatcgcgt
    17041 gccgtcgaat ctcactaccc gtctacgcca gctggaaaca gaactggggg tagatctgtt
    17101 tattcgcgag aatcagcgtt tacgtctctc tcctgccggg cataactttt tacgctatag
    17161 ccaacaaatt ctcgcgttag tggatgaagc gcggagcgtt gtcgctggcg atgagccgca
    17221 aggtttattt tctcttggtt cgctggaaag caccgcagca gtgcgcattc cggccacgct
    17281 ggcggaattc aaccatcgtt atcccaaaat tcagttttca ctttccaccg gcccttccgg
    17341 cactatgctg gaaggagtgc tggagggaaa actgaatgcg gcatttattg atgggcccat
    17401 taaccatact gccattgacg ggataccggt ataccgcgag gagctgatga tcgtcacgcc
    17461 acaaggtcat gcgccagtaa tccgcgccag tcaggttaac ggcagtaaca tttatgcctt
    17521 ccgcgccaat tgttcgtatc gtcgccactt cgagagctgg tttcatgctg acggtgccgc
    17581 tccgggaact attcacgaga tggagtcgta tcacgggatg ttggcctgtg tggtcgcagg
    17641 agcaggcatt gcgcttattc cacgctctat gctggaaagt atgccggggc atcaccaggt
    17701 tgaagcgtgg ccgttagctg agcaatggcg ttggttaaca acctggctgg tctggcgtcg
    17761 cggtgcgaaa acgcttccgc ttgaggcatt tattcaacta ctggatgtgt ctgactcggc
    17821 aaaacagagt taccaatgag ctattttgat agttcttgcg gctaatatgc tctatatagt
    17881 gatgttccga tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca
    17941 attacatatc tcttcctgat gattttcccg ttcgtaatat tgcaccacag gtaaaagaag
    18001 ttttaaagga ctttattgat gcgctaagta caataatatg tgatgaagag tggcgcacgt
    18061 ctttaaacat caactcagcc acaa
//
LOCUS       DAZFYC010000099        18063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFYC010000099 DAZFYC010000000
VERSION     DAZFYC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.099"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..134)
                     /locus_tag="ACHKW5_003704"
     CDS             complement(<1..134)
                     /locus_tag="ACHKW5_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFO7513025.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     "
     gene            complement(321..704)
                     /gene="traM"
                     /locus_tag="ACHKW5_003705"
     CDS             complement(321..704)
                     /gene="traM"
                     /locus_tag="ACHKW5_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO7513026.1"
                     /translation="MAKVNMYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFLRNDDE"
     gene            1025..1627
                     /locus_tag="ACHKW5_003706"
     CDS             1025..1627
                     /locus_tag="ACHKW5_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7513027.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTEHQNQ
                     RRLAYASDVYRIYTGIKSSKGIQIPATKKSLPEINSVQNN"
     gene            complement(1748..3085)
                     /locus_tag="ACHKW5_003707"
     CDS             complement(1748..3085)
                     /locus_tag="ACHKW5_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO7513028.1"
                     /translation="MPLLNDLLDFSDHPLMPPPSAQLFAGHLPVEWIQHCLTLSAHAT
                     VRRRRLPGDMVIWMVVAMAFFRNEPITDVVRRLNLSADGEAGMNLLARSAVTQARQRV
                     GAAPVEWLFRQTAQTWGSERYLKDDWHGLQLFAIDGAPFRTPDEPELREYYGSANTST
                     ERQSAYPVMRLVALMNLGSHILLNAVTAPYRRSETVPAHSMLATIPDNSITLFDKLFY
                     SADLLLTLNQQGCNRHWLLPAWKNIAAETEESYGPGDRLLKLKVSLQARKKNPALPEF
                     WYARAVTYEVNGVEKTVQTSLPADRYKAKEVAELYHSRWEIEVGFRNLKSSLLNNALV
                     LRSRKVELLEQEVWGMLLAYNLIRREATKAAEKHKKAASEISFKFAFQFIATEMIVLG
                     NTASPGTIPKRLEHLRGALEVVFITKRPRPSRPRAVKISKTRYPVKRSTAPLK"
     gene            complement(3364..4181)
                     /locus_tag="ACHKW5_003708"
                     /pseudo
     CDS             complement(3364..4181)
                     /locus_tag="ACHKW5_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(4300..4586)
                     /locus_tag="ACHKW5_003709"
                     /pseudo
     CDS             complement(4300..4586)
                     /locus_tag="ACHKW5_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4611..4817)
                     /locus_tag="ACHKW5_003710"
     CDS             complement(4611..4817)
                     /locus_tag="ACHKW5_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7513029.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQIPAKRQNRGSRNSKDDGG"
     gene            complement(5363..5554)
                     /locus_tag="ACHKW5_003711"
     CDS             complement(5363..5554)
                     /locus_tag="ACHKW5_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513030.1"
                     /translation="MNINTETREILRNYRAVINVRRRDMGQKPLTTAQIVDEICDSVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(5551..5973)
                     /locus_tag="ACHKW5_003712"
     CDS             complement(5551..5973)
                     /locus_tag="ACHKW5_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7513031.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(6020..6421)
                     /locus_tag="ACHKW5_003713"
     CDS             complement(6020..6421)
                     /locus_tag="ACHKW5_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7513032.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLGFLYAEASVYVLAKQMMP
                     EYEGGFWHFIRLPDGDRFHLVNGANWFDRTVSADAAGIILTSLVINRQLWLYHDSGDA
                     GLTHLYRMRDAQSWSHIEVHPECNAIYAALD"
     gene            complement(6838..7614)
                     /locus_tag="ACHKW5_003714"
     CDS             complement(6838..7614)
                     /locus_tag="ACHKW5_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513033.1"
                     /translation="MNENTTLNALICRHARDLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTLRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCLEHILSSGLACDV
                     LRIPDEPPRQWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(7660..8146)
                     /locus_tag="ACHKW5_003715"
                     /pseudo
     CDS             complement(7660..8146)
                     /locus_tag="ACHKW5_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(8106..8327)
                     /locus_tag="ACHKW5_003716"
     CDS             complement(8106..8327)
                     /locus_tag="ACHKW5_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513034.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(8328..9011)
                     /locus_tag="ACHKW5_003717"
     CDS             complement(8328..9011)
                     /locus_tag="ACHKW5_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO7513035.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(9088..9393)
                     /locus_tag="ACHKW5_003718"
     CDS             complement(9088..9393)
                     /locus_tag="ACHKW5_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513036.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSSVGLTRYRTR"
     gene            complement(9397..9814)
                     /locus_tag="ACHKW5_003719"
                     /pseudo
     CDS             complement(9397..9814)
                     /locus_tag="ACHKW5_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9737..10857
                     /locus_tag="ACHKW5_003720"
                     /pseudo
     CDS             9737..10857
                     /locus_tag="ACHKW5_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527236.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(10998..11960)
                     /locus_tag="ACHKW5_003721"
     CDS             complement(10998..11960)
                     /locus_tag="ACHKW5_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO7513037.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGEIKTFTLKSGKQAKFIKT
                     VVLSGDIESKTFVDVSVNGRDQTMLSRESVSDITRTIKLQQFFPAIGREVDGRIEVLD
                     GTRRRAACIYNNVKFEILVTKDSIDIADARQLAKDIQTAREHSLRELGKRLEVTYGAS
                     MIKEEIAQAESISPAKVTRAFQAASVPDEMISVFPIINDISLSDYQFLLKLSEKATNK
                     KMSVEEFMDRVRQKLMALPDYPTIEKSKILAVFRSEGKLLTDRPVKTVETEKLKEFSD
                     RNQFARKKTDPRKRLVVYEFSRISNEVQLEIDEAIKNILRKLPE"
     gene            complement(11957..13162)
                     /locus_tag="ACHKW5_003722"
     CDS             complement(11957..13162)
                     /locus_tag="ACHKW5_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7513038.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTEYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEESGYEFNKKRVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDVHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNNLDAETLREAVIRPTMIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGFKPSEVLRKTIIDRIADDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTSKRDHETSYSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            13533..13706
                     /locus_tag="ACHKW5_003723"
     CDS             13533..13706
                     /locus_tag="ACHKW5_003723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513039.1"
                     /translation="MMEIKAQQFVTSTGRQVLTDNGQQGMGGVAEAIFANCAELDNDQ
                     LNEIIEWVRLYQR"
     gene            13880..14131
                     /locus_tag="ACHKW5_003724"
     CDS             13880..14131
                     /locus_tag="ACHKW5_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7513040.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYC
                     VPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLDKYL"
     gene            14128..14415
                     /locus_tag="ACHKW5_003725"
     CDS             14128..14415
                     /locus_tag="ACHKW5_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7513041.1"
                     /translation="MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKL
                     RGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGKRERSDVYNLASERMR"
     gene            14417..14822
                     /locus_tag="ACHKW5_003726"
                     /pseudo
     CDS             14417..14822
                     /locus_tag="ACHKW5_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15468..16445
                     /locus_tag="ACHKW5_003727"
     CDS             15468..16445
                     /locus_tag="ACHKW5_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO7513042.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVTGDKVE
                     LPFVEFAKLCGIPSNQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(16729..17469)
                     /locus_tag="ACHKW5_003728"
     CDS             complement(16729..17469)
                     /locus_tag="ACHKW5_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7513043.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(17593..17712)
                     /locus_tag="ACHKW5_003729"
                     /pseudo
     CDS             complement(17593..17712)
                     /locus_tag="ACHKW5_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            17784..18046
                     /locus_tag="ACHKW5_003730"
                     /pseudo
     CDS             17784..18046
                     /locus_tag="ACHKW5_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4561 a         4649 c         4426 g         4427 t
ORIGIN      
        1 ttgcaaaaaa taaattttcc agtttcgtcc ctgatacagg ctggatttgg ataatcctga
       61 ataactgccg tcagattttc cagcagatct atttgacgag catgtttttg ttgaatacga
      121 tccatcggat acataggaac ctcctcacaa aggattctat ggacagtcga tgcagggagt
      181 tcacgtctcc ctgcatcggc gattttcaat ttcaaatttt aacgtggcat taaccacgta
      241 attaatatac taattatctt tcgtactgtt acctttttaa atcataaaaa ctctgatatt
      301 tgaacgaagt caaatttctt ttattcatca tcatttctta gaaaaaatcg ttccatctca
      361 gatgatacct tctccctgat atcttcaacc atattggcat attcaaactt tggatttccg
      421 gagacatgag gactgagaga ctcaataccc aaaattttcg ctactgatga ttgtgttttt
      481 acaacgcatt caagaagcaa tttattaaac tcagtctgat taaatgcaga ctctttacgc
      541 tccatctgag cctcatgtac acgaagcccc agttcaagaa gcattgaagc tgttgctgaa
      601 aaactgacat ctttttccct tgccccttcc tgtcgacgct tctcaataat cgcatttatt
      661 ttttcatagg catcattgct gatatacatg ttcaccttag ccattaaaga ttaccttttt
      721 tgaacataaa ataaagttaa tttagaaaca ctaaaattca taatgcaaac agggacgcac
      781 cgctagcagt gcccctggcg gtatcctata aagaaacaca ccgcgccgct agcagcaccc
      841 ctaatataaa ataatgtttt ttataaaaat agtcagtact acccctacaa agcggtgtcg
      901 gtgcgttgtt gtagccgcgc cgacaccgct tttttaaata tcataaagag agtaagagaa
      961 actaattttt cataaaactc tatttataaa taaaaatcag caaaaacttg tttttgcgtg
     1021 gggtgtggtg cttttggtgg tgagaaccac caatctgttg agcctttttg tggagtgggt
     1081 taaattattt acggataaag tcaccagagg tggaaaaatg aaaaaatgga tgttagccat
     1141 ctgcctgatg tttataaatg agatctgcca tgccactgat tgctttgatc ttgcaggccg
     1201 ggattacaaa atagatcctg atttactgag agcaatatca tggaaagaat cccgttaccg
     1261 ggttaatgcc atcggtatta atccggtaac gggatatggc agcggactga tgcaggtaga
     1321 ttcccagcat tttaacgaac tggcccgcta tggaattaag ccggaacatc tgacaacaga
     1381 tccctgcatg aacatttata ccggtgctta ttatctggca atagccttta aaaaatgggg
     1441 cgtctcctgg gaggccgttg gtgcatacaa tgccggattc aggaagaccg aacaccagaa
     1501 ccagagacgt cttgcctacg catcagatgt ttaccggatt tataccggga taaaaagcag
     1561 taaaggtatc cagattccgg ccacgaagaa atcacttccg gaaattaaca gcgtgcagaa
     1621 caattaatct tacagacatt gtcagatacc ggttatgccg caaaagcggc atggaaggcc
     1681 acacacaaaa atatcatatg agtgacaata ctgactggcc gaagccccgc gaagcgggca
     1741 atgccgttca cttaagcgga gcggtgctgc gtttcaccgg ataacgggtt tttgatatct
     1801 taactgccct cggccttgat ggtcgggggc gttttgttat gaacacaact tccagagcac
     1861 cccgcagatg ctccagtcgt ttcgggatgg tccccggtga tgccgtattt cccagcacta
     1921 tcatttctgt cgcgataaac tggaacgcga acttaaagct gatttctgac gccgcttttt
     1981 tgtgtttctc agccgctttt gttgcctcac gccgtatcag gttgtaagcc agcaacatgc
     2041 cccacacctc ctgttccagc agctcaacct tacggcttct cagcaccagg gcattgttca
     2101 gcaggctgct tttcaggttt ctgaacccga cttcgatttc ccatcgtgaa tggtaaagtt
     2161 ccgctacctc ttttgctttg tagcggtctg ccgggagtga tgtctgcacc gttttctcca
     2221 caccgttcac ctcataagtc accgcccggg cgtaccagaa ctctggcagt gcaggattct
     2281 ttttcctcgc ctgcagcgac accttcagtt tcagaagcct gtctccggga ccgtaacttt
     2341 cttctgtttc tgccgcgata ttcttccacg caggcagtag ccagtggcgg ttacaaccct
     2401 gctggttcag cgtcagcagc aggtctgcgc tgtaaaagag cttgtcaaac agcgtaatgg
     2461 agttatccgg gatagtggcg agcatggagt gagccggcac ggtttcgctc cgccggtaag
     2521 gcgcggtcac ggcattcagc aaaatgtggc ttcccagatt catcaacgcc accagacgca
     2581 tgaccgggta ggcgctctgc cgctcagtgg atgtgttggc agagccataa tattcacgca
     2641 actcaggttc atcaggtgtc ctgaacggtg cgccatcaat ggcaaaaagc tgcaggccgt
     2701 gccagtcatc cttcaggtaa cgttccgagc cccatgtctg tgcggtctgg cggaagagcc
     2761 attccaccgg ggcggccccc acgcgctgac gcgcctgggt gacagcgctg cgggccagca
     2821 ggttcatccc cgcttcgcca tccgcgctca ggttcagacg gcgaaccaca tcggtaattg
     2881 gctcattgcg gaaaaaggcc atggccacca ccatccagat aaccatgtcc cccggtaaac
     2941 gacggcggcg aacggtcgca tgcgcagaaa gcgtcagaca gtgctgtatc cactcgacgg
     3001 gaaggtgccc tgcaaacagt tgtgcagagg gcggtggcat aagcggatgg tcactgaaat
     3061 cgagcagatc attgagaagt ggcataagaa aacggctccc tgttgtggaa gccgttatag
     3121 tgccgcagtt taaggatcgg tcaaccgatc cttaactgat cggcattgcg cgaagcggga
     3181 acccatctta tatataaccg gtaactgcgc agcctcctgc cagaccattt ttcgcgggca
     3241 caatatttac agacaaccgg aaagccagtt gatgaaattg aggtatatca gacgacacag
     3301 cgatgccgaa gaatgataat ataagtaaag ccccgaaaat ttttcggggc tttacttata
     3361 ttatcatgac ttccactttc tgaactgttc ggcaatcacc cataacgcct tgttgattct
     3421 tatatcacca tcaatacctg taatggctct tgtcctggta tttttcccgg aggcacttcg
     3481 ccccgataat ccacctttta tcatattctc ctgagcccgc tgccaggttg tccagagatc
     3541 gttctgttta ttctcccgac gacggggagt aattatctgt tcaggcgtca ctggcgtttt
     3601 attttcatct tcatacctga ccatcagtgc agctctgcca aataaatgtt gctcgtcact
     3661 gttaagatga atttctttca tcgcctccat attatcagtg accttatcaa agacaccgag
     3721 tacttcatac gctccctcga taacctgccc gacaatatca cctttatgtg gaacgcggat
     3781 ttcgccaaaa ttatttccgc acaccaggcc atttgtgcag acaaaacgaa aaattccggg
     3841 gatcatctga taactggatg aaccatcatg tgaattaagc aggataattt caggaacttc
     3901 ctgcccgtta atatgccctt ccctgcgaag acgtaacata tgtttactgt attcgcgacg
     3961 tcccaaatca cgaacccgac tctgacaggc aaagaatggc tggaaacctt catcacgtaa
     4021 cttattgatg atattgttgg aatatacgta taacgttcac tccgggactc atgtttatca
     4081 ccggaaaata ccgaaggcac gaactgcatt aattcatcat ccgttaaagg acgttcacgg
     4141 cggatggaat tataccgacc aaaacgggaa gctaatcgca taacagctac tcctttatga
     4201 aagaataaat ttaataaaaa acttttccgc ttaatatcat ccctcccggt cactattaac
     4261 aagattgcaa caatcaggag aaatattcat cacatctggt cagcagatcg cccggatatc
     4321 gaggccggca cgcttaatct gacgcaggaa ctgtttgaag tgatcggctt ccgcaatgtt
     4381 gatatggcgg aactgcgtat ccacaccccg gctgagcgcg tagctatagc tgaaggccat
     4441 cttttccggg tggcttttgc gatatttttt cagctcagtc aggctgctga attccaggac
     4501 gtcccactct tccccggtgt ttttttcttc ccggcaacaa gaagcgtgta cgacgcggtg
     4561 ttaacgtgga tatttcgggt actcatcctg ctttctcctc tctctggttt tcagccccca
     4621 tcatcttttg agtttcggga gccgcggttt tgacgttttg cggggatctg ccttttgttc
     4681 cctccggtat tcaccatcac ctttttgaga accttcgccg caaggggcat cactgccacg
     4741 gccggaaacg actgtccggg ggcagaccgc aaaagttttt gcctgctttt gggcaaaaag
     4801 ttttgcggga tgtacatttc accctggact ggcggagacg tctttgcccc cggcgacagg
     4861 ggcttaaaca ggtgaatggc accggacaca acaacgggca gggacccgcc gcaacgacgg
     4921 agagcgcgtg cgcgaaacgg cgttgcccct tacatcccgg cggccttttg ccggaatgcc
     4981 aggagcgcag gccacggcgc gacagaggcg gtgcgaccgg aacggtcgct gcaagccagg
     5041 gtttcgcgtc agggatggaa gcccgccagg gcggagacag cttcgctggc tccgttcacg
     5101 acagcccgcc ccgccagggg gacgcccgga aaagccataa acaggagagg aacggaaaga
     5161 agaggaagag gaaaaagaag aagaacaata acaagcacgg taaccataga aaaataacat
     5221 cagaaaaaaa cattccggac acatcacaaa caggaagggt aacgggcgac gcgtcagcat
     5281 ggcccgcttc ccttccggca ggcgtcagcc tgccaggggt tttcgcgcgg gctgacaggc
     5341 ggcctccacc gcctgccctg tatcacctgt ttctgctgcc ctgaaggata tagtgaccac
     5401 cgaggaaaac cgcctgctga ttcgccacag aatcgcagat ttcatcaaca atctgcgcgg
     5461 tggtgagcgg tttctgcccc atatcacgac gccgcacatt aatcacggcc ctgtaattac
     5521 gcagaatttc gcgggtttcc gtgttgatat tcatgacatg tgctccccgt ccagcgcctg
     5581 taacacaacg tccattgctt cgcgttcttc cctgacgaat gcgtcaccgt cgcccccgtt
     5641 ctcactcccg acggcataca ggcgcttcat ttcactgcct gccagcgcca tcactttttc
     5701 aagcatcact gccgggactg tcacctgacg gctggcacgc ttttcttcca tcagttcacg
     5761 gacaacgtcg tgaacaatgc tgacatccgc accatgctca aaagcatcgt ccagccgttg
     5821 catgacggtt tccagctcat cgtctgtcag actccagtcc cgggctatgt gacgcacatc
     5881 cccacgggtc agcaccacgg caaaaacgca ttcactgtcc ggcacgtctg tatccagcac
     5941 ttcgcggata atttctttaa cagtacagta catgttctcg tctcctttac aggggcggca
     6001 ttgccgcccc gtccgttaat cagtccagtg ccgcgtaaat cgcgttacat tcgggatgaa
     6061 cttcgatgtg gctccacgac tgcgcatcac gcatccggta caggtgtgtc agtcccgcat
     6121 caccgctgtc gtggtacagc cacaactggc ggttaatcac aagggaagtc agaatgatgc
     6181 ctgcggcatc ggcgctgacc gtgcggtcaa accagtttgc accgttaacc agatgaaaac
     6241 ggtcaccgtc cggcaggcgg atgaagtgcc agaagccgcc ttcatattcc ggcatcattt
     6301 gtttcgccag cacgtacaca gacgcttcgg catacagaaa acccaggcca aacaggtaag
     6361 gcagaaagga gagacggtcg cactcttcaa cattcagagt gacaggtttc gcatattgca
     6421 tgatgttttc ctccgttgtt cacatattcc gcgacaccgg gcggtgccgt gcctccataa
     6481 aagccgggcg ataaggcggg gatgcagggg cgcaagggaa gccgcagcct gacgcacacg
     6541 gctggcggga gttggagggt gagaccgcga aacctgagcg ggtgaacgac cggaaaatca
     6601 cggcagacgg gaaggaggga ccgggtgacc gcagcggcga agcgtaccct tgcatccccg
     6661 ccagcccggc taagatggag gcacggcaca gcccgcacac aacacggcgg ccacataact
     6721 gaaaacccac aaggccatgc cccgcatggc cgggtaaagc ggagccggtg gcgacttcct
     6781 ttgagctctg tccggggacc ggcaggaaaa aggcggggat ttaccccgcc aacgtcatca
     6841 tttccggatt tttgccaggg catccgccat gctgcggatt tcggcccggg cttcccggac
     6901 aacttcccgc agaacaccac ggtcaaacca ctggcggggc ggttcatccg gtatacgcag
     6961 cacatcgcag gccagcccac tggacaggat atgttcaaga caatcgctga ccagatacat
     7021 ttccaccgcc gaaaaacgtg cgccccggtt caggatggca tcgagcacca cgggcaggtt
     7081 ttcgtcgtcc tcatcgagcc agcaggggtg atcgctttcc ttcacgacta ggcgaaagcg
     7141 gcgcgtctgg cgggtcagta acgtctgacc ggtggtggtc agcgccgccc ggatacgcag
     7201 cgtatcttct gccacccggc aactgacact gcgtacagca tcacgcaccg ccgcaagtac
     7261 agccctgatt tcgtcttcgg tcagccattc accggcatac ggaaaagaca cctgcgttcc
     7321 gtctgccgga agtaccggca gcgactgcca ctgactgccg gacagtacaa gttccgcccc
     7381 ggttccggtc agtttgtgaa tagcggaggc caggagcggc ggcagtacgc cgccgtgacg
     7441 gttgataagt aacgtcgcca gccggggcgc atccagcaga acataaatgc tgatccagcc
     7501 cggatacttc gggttccgct ggtcaacatc agtctcttcc ggccagccct gcgccagcag
     7561 caggtcgcgg gcgtgacgac aaatcagtgc attcagcgtt gtgttttcgt tcatgattaa
     7621 tcgttctccc tgtatcgccg cccggaggcg gcgataacat cagtcgtttt cgtgcgtgtt
     7681 gtttttcagc aggatgcggg cctgattaac caccgcctga cggcgggtga cgaattccgg
     7741 gacggcgagg ccgttatcac gggcggccca ttcacgcttc cacaatgcct gttcagcggt
     7801 ctgaatcagg gacgccagca gttcagcagg gacggtcacc tggcggtttt cactcacgtt
     7861 gctgataatc tccatagccg caccggaaga aataccggac tcagtccgct ggtcttccgg
     7921 gatgtcctcc agccgcgcca ggacggtcct gatttcatga tcggacaggg aaatatccat
     7981 tccatcagcg aggacctgaa tctcttccgg ggaccagaca accagcatca gcggagtgtc
     8041 tcccggatac tgcgctgcca actcccggca gagcgtttcg catgttccgt acatactctc
     8101 tcccctcaat ctttgaagca ggaatccagt tccagaattt cggtatatgc ctccatcaag
     8161 aggcggtttg cgcgaaacag cgtctgacgc accagacgtt ccggcagggt gtcggcatta
     8221 aagctgtacc gctgctccat ctcattcatg gttgtacgca ggtcgcacat ggcattggcc
     8281 acctccgcca cttcggcgcg cagtttttcg tgtccggcat agttcattta cgcagcctcc
     8341 ggttcaaacc agttatcatt cgcggccccc tgctgcatgg cccgttgcac ggcggccagt
     8401 cgttgctgtc cggcacggtg atactgttca agcagctcga taccgatata ccggcgtccg
     8461 gactgaaggg cggccacgca ggttgagccg ctgcctgcaa acgggtccag cacaattgcg
     8521 ttcggatgtg tgaagctctc aatcagtggt tgcaggctgg taacgggctt ttccgtcggg
     8581 tgatggcggt tgcctgaata tttccagccc agcacgtcgg gcaatggctt ctgaggcagc
     8641 gccggacgcc cttttgccag gatgtaagcg cattcgtggc gatagcccac atatgcggcc
     8701 ttcgatgtgt aggttttggt aaataccagg tggccaacca cgctgaatcc cgcatttttc
     8761 caggcggcca taaagcgatc gacgcggttc cagccataga agctcaccat cagcgcatct
     8821 tttttcagca ctcggtacat ttcattacag gccggttgca gccattcatc ggttttatcg
     8881 ccggcgatgg tacgcccctg acggtcacgg aaaccgacga gatatggcgg gtcggtgagg
     8941 atgaaatcga cagcattgcc cggaaacgtg gccataacac gcacgcagtc accctggata
     9001 aaacgggaca taacagcact ccttctctta cagagggcac cgggagggcg cccctgtggc
     9061 gcccgttccc cgctgcctgt gggctgttca ccgcgtccgg taacgggtca ggccgaccga
     9121 ggaataccgc agccgtcgtc tttttgacgg cgaggatatg ccgcgaaagc ggcttgaagg
     9181 gcgttcatca gaaaagggcg acgggctggc gtacactgtc accaacgcca gccgggagcg
     9241 gtaaaagcgc ccgtcccgtg cccggccgga cgcgggacac tgaccacagg cagcgttcct
     9301 gccgcgccgc gtcagccggc gcagtaaaaa gaggagcccg gcgacatcct ttaccggaaa
     9361 agaaacgggc ggttgcccgc ccgtctccgt catacttcat cctccataat gcgccacatt
     9421 gtcgactatc cacgccggtc cggtgacttc cggcagctca tcgtcatctg tcggggaaga
     9481 ccattcaagt tcccccccac agcacatcaa cgagctctcc acgctcatac aactgccagc
     9541 cacagaaatc acagccctgt tcggcatacc agtgctccag tgtgcaactg aaacggcggc
     9601 ttaattctgc aataacgtcg ctttctggct gacaccacgg cgtgtcaaaa tccacctgaa
     9661 tgaagttgtc tctctgactg ctgatattca cctcataccc cacaggccac ttcacgccat
     9721 actgttcggt ataccagtga tatgcagaag gaacaccgtt cagaacacca ccgttaaagc
     9781 cgtttacttc aacatccagg cggctgggca tcaaaatggg gtaccgtggt ttcagctgaa
     9841 gtcggtgaaa ttttcggagc cggtcatcgt cttctccagt aattacccgc tatacgcatc
     9901 gatgtcgaac agggtcatgg ttcatctgga ggcgctggca ccgcgcgttg agcagtactc
     9961 gattgacgag atgtttctcg acgtgcgagg tattgacagc tgcatcgatt tcgaggattt
    10021 cgaccagcag ctgcgtgaac acgtcaggaa cgggactggc ctgactatcg gtgtcggaat
    10081 ggggccaacc aaaacgttgg caaagtcaac acaatgggct tccaaagagt ggccacagtt
    10141 tggtggcgtg ctggcattaa cgccggacaa tatccgccgt gcggaaaaac tgctgtcact
    10201 gcagctggcg cacgaaccct gtatttatca ggaagaactt caatgtggtt ctggagagaa
    10261 aggtccggga actcaatgga gaaagctgta tttcactgga ggaagaaccg cccccccaaa
    10321 cagcagatag tctgcagccg gtcctttaga gagcgcgtca cgacatatga ggccatgcgc
    10381 taggccatct gtcggtacgc tgagcgtgca gctgaaaagc tgcggggcga gtgtcagttc
    10441 tgcagacata tcgccatttt tttgaagacg tcaccgttcg cagtaaacga accgtattac
    10501 gggaatgtgg ccagtgaaaa actgctcatc cccacgcagg atacacggga tatcattgcc
    10561 gctgccgtta gaattctgga caggatatgg gtggatggcc atcgatatgc aaaagcgggt
    10621 tgcatactca acgatttcac tcctaccggg gtttcgcatt tgaacctttt cgatgatact
    10681 cagccccgga gcaacagtaa ccagccgttg aaagtcgtgg acagcattaa ccattccggt
    10741 ctggggaaag tgtggtttgc aggccgaggc attgcgccag aatggcagat aaagcgtgag
    10801 atgtcgtcac cggcatacac tacccgctgg aacgaactac cggtcgccag actttgaaaa
    10861 aaagaaattc atggtgattt cactatgaaa gaattaaaaa aaatacttta tattcagtaa
    10921 aataagcatg aaaaaagcgt ggtgaaaatt gcaaaaaaag gatgccattt ggcatcctga
    10981 tattaacccg gcatatccta ttcaggcagt tttcttagaa tgttttttat agcctcatcg
    11041 atttctaact gcacctcatt agatatgcgg gagaactcat aaacaacaag acgctttctg
    11101 gggtcagtct tctttctggc aaattggtta cgatcagaaa actctttcaa cttctcagtt
    11161 tccactgttt ttacaggccg gtctgtaagc aattttcctt cagacctgaa gaccgccagg
    11221 attttgcttt tctcaattgt tggatagtca ggtaaagcca tcagtttttg gcgaactcta
    11281 tccataaact cttccactga catttttttg ttggttgcct tttcagacag tttcaacaaa
    11341 aattgatagt ccgacagcga aatatcattg attatcggaa atacagagat catttcatcc
    11401 ggtactgatg ccgcctgaaa tgcgcgtgtt acttttgccg gagagatgct ttcagcctga
    11461 gcgatctctt cttttatcat acttgcccca taagtaactt caaggcgttt tcctagttct
    11521 cggaggctat gctctctggc tgtctgtata tcttttgcaa gttgtctcgc gtcggcaata
    11581 tcaatactgt cttttgtaac cagtatttcg aattttacgt tgttatagat acaggcagca
    11641 cgacgacgcg taccgtctaa tacttcaatt cgtccatcta cttcccgacc aatagccgga
    11701 aaaaattgct gaagtttaat cgtgcgagtt atgtcactaa ctgactcacg cgaaagcata
    11761 gtctgatcgc gtccattaac agatacatca acgaacgttt tagattctat atctccgctt
    11821 aatacaaccg ttttaatgaa ttttgcttgt ttccccgact taagagtaaa cgttttgatc
    11881 tctccgtcac cttcaagcat gcgagcgaac tctgaattgc tattgccgag aactcgccct
    11941 cgagctaaaa cctttttcat ttggatgctc cccgaatgta ttcaatgcgg tcaaaaacgg
    12001 ctttagtaaa tcgttccgct tcggtacgag cttttttcaa tgcctctgca cttcctggat
    12061 acgaaacagg gttagcactg atgatagtgt caaaagattc accacatcgt tcgaacccgt
    12121 ccagtctcgg aagcgatgag tcaagaatat tactcgcata cacttcacga gccaggctat
    12181 aggaagtttc gtgatcgcgc ttactggtca ttttggacat aaaccctatg ctcgcactca
    12241 ggcggggctc aacgccttct tcttccaaac gttctagcat ttcaggtaaa cgagtcaaat
    12301 atttgagagt tgaatggaaa tcaacttgcg ccgggggagt aggggtgaga agtaaatcac
    12361 tcgcagccag gccattgaga agaaatggat caagatgagg gccagtatca ataaaaacaa
    12421 aatcataatc atcagcgata cggtcaatga tggttttcct gagtacttca gaaggcttaa
    12481 aaccaggaag gtgttcagca accaaagact cccattggct tgctacgaag ccgtcatcaa
    12541 tagaagcagg gatgacatca acgccaggaa tcatagtggg tcggataacc gcttctcgca
    12601 gtgtttcagc atcgagatta tttagcattg cttgggcggc agtttcaagg actgtaccaa
    12661 tactatgggt atggtccaaa aacatagtgc tggacgcctg cgggtcaagg tcgatcacaa
    12721 ggatacgaag gtcatgacgc agcaaatccg gatgtactcg tagtgcatgc gccagcgtta
    12781 ctgtactgac ggtttttgaa accccacctt tcaagttgac gacaaaaatt acgtaaggtt
    12841 ctttatgaac atcgcgatat tttggtatct gtcggtgagc gtaaatatca ataacgttct
    12901 gaattgttaa tgcatactgc tcgacgttgc ctacccgctt tttattaaat tcatagccac
    12961 tttcttccat ttctttaacg gccagttcta cgatgcgtcg gctcagcttc ggcaattttg
    13021 cgaccgcatt acgagtatat gtttgatgat actctgtcag attgaattct tttctttgct
    13081 cctcaatatc ccgactcatg gcctgaagta gagcacttgc tctgagcgct atggtaccga
    13141 cactaccgta atctcgtttc atagttttcc tctgagatag attccctgcc catatattgc
    13201 gtacaaagtt caatcacatt gtacacaaat atgaaagagg tacaatatat tttgcattgc
    13261 cattaaatta tcgtacgttg caagtcacca ttcacatcaa accgcatagt tagacattgt
    13321 ctctgagcag gcgaggggtt agacaagacc gttgggcgcg tcagagtgtt atattaggat
    13381 acgggtaggt gacacccacc tctgtcacag aacggacatt cattgatgcc atgccagaat
    13441 ggacgttcag gttattccgt ccagttctgc tggcagcacg agatctcccc tggtatagtg
    13501 atgccacggc aaagtgctca aacagggata atatgatgga aatcaaggct caacagtttg
    13561 tcacatcaac ggggcggcaa gtccttactg acaacggaca acaaggtatg ggcggcgtgg
    13621 cggaagcaat ttttgcaaac tgcgcggaac tggataacga ccagcttaac gagatcatcg
    13681 agtgggttcg gctctatcag cgctgaatgc catcatcagg ctgcgcaagc ggcctttttt
    13741 acgccccttg tttaattccc gcactacctg gacattcagg tgattctgtc catctgtata
    13801 aaaaacaacc aaaagacttg ttaacaggtc atataaggag tatctttgag actggttaaa
    13861 tagtcttgaa aggtggccta tgcctaacat tattctcagt gatacaagcg ccagtgtcag
    13921 cgagctgaag aaaaatccga tggcgacagt cagcgccggt gatggtttcc cggtcgctat
    13981 cctgaaccgt aatcagcctg ctttttactg tgtacccgca gagctgtacg aaaagatgct
    14041 cgatgccctg gacgatcagg agctggttaa actggtagcc gaacgcagca accaaccgct
    14101 gcatgatgta gacctagata aatacctatg aggtatcagg taaaattcag ggaagatgcg
    14161 ctgaaagagt ggcaaaaact ggacaagact attcagcaac agtttgcgaa aaagctaaaa
    14221 aagtgctgtg acaatccgca tattccttcc gcaaaactgc gcgggataaa ggactgctac
    14281 aaaataaaat tacgtgcgtc aggttttcgc ctagtctatc aggtgattga cgaacaatta
    14341 attatcgctg ttgtagctgt gggtaaacgt gagcgcagtg acgtttataa tcttgccagt
    14401 gaaagaatga gataattcac ggcggattat cggttcagcc ctctcattaa ccgctgatgc
    14461 tgagttgatg tgtagggctt tgcgtaatgc actggaaaca cgaccgcgtg atggtcgcct
    14521 gctgtttcat tcagatcaag gtgttcagta taaaagcaat aaatacagaa agttacttta
    14581 tggggtaatg caaagtatga gtcgcagagg gaattgcctg gataatccgc cgatggaaag
    14641 aatctttcgc agccttaaaa gtgaatggct tccgaaaggt ggttatggtg attgccatgc
    14701 agtacgcgaa taaatcagtg gataaatgct tattacaacg tttatcgacc tcatacaaac
    14761 aatggtggtt tatcgccttg ccgtatgaag aaaaatggaa acaggttatt ccggtgtcct
    14821 gattttatga tccactacac attcctgacg aacgccactg cataaatcac attgcacatc
    14881 ctcagagagt acgcagtata ggaggggtat atatacatta tcggtatatc aggcgacttc
    14941 tgtctgtatg ttgtcggtgc tcgccgcaga ccggtgaccg ggcgaagccc gcgaaccggc
    15001 gaggaagcgc aatgtatctg tagttacagt gagcaggtac gccctgaagc cctttcataa
    15061 aacggttgtg tgcgctgtcc ttcgggaaga tttatccaca tatccagagg cccgatccag
    15121 taaacagatc catgaatgat caataaagga tccattaaag atccccatac cgctgcaaac
    15181 cttgccactc atgctccaga accacgatca cataagctgt ggcatgttga tgataaactg
    15241 taacatgctg atgataagct gtattcagta atacatatac tgacgtaagt taatgacata
    15301 aactatggtc agtacaccag actcagttgt taaatacaga ctgcaggttt ttcttcagtc
    15361 agttgacggg ggtctgacac acgattttct gtttattctt ttactgtcca caggcaggag
    15421 gctttctgga aaacgaaaat tcagacatca aaaaactgtt cggcgaggtg gataagtcct
    15481 ccggtgagct ggtgacactg acaccaaaca ataacaacac cgtacaacct gtggcgctga
    15541 tgcgtctggg cgtctttgta ccgaccctta aatcactgaa gaacagtaaa aaaaatacac
    15601 tgtcacgtac tgatgccacg gaagaactga cacgtctttc cctggcccgt gcagagggat
    15661 tcgataaggt tgagatcacc ggcccccggc tggatatgga taacgatttc aagacttggg
    15721 tggggatcat tcattccttt gcccgccata acgtgactgg tgacaaagtt gaactacctt
    15781 ttgtcgagtt tgcaaaactg tgtggtatac cttcaaacca gtcatcacgc aggctgcgtg
    15841 agcgcatcag cccttccctg aaacgcattg ccggtaccgt gatctccttt tcccgcaccg
    15901 atgagaagca cacccgggaa tacatcactc atctggtaca gtcagcctac tacgatactg
    15961 aacgggatat tgttcagtta caggctgatc cccgcctttt tgaactgtac cagtttgaca
    16021 gaaaagttct tctccagctt aaggcgatta atgccctgaa gcgacgggag tccgcccagg
    16081 cactctacac atttatagag agcctgcccc gggatccggc accgatatcg ctggcgcggc
    16141 tgcgtgcacg cctcaatctg aagtctccgg tattttccca gaaccagacg gtcagacggg
    16201 caatggagca attgcgtgag attggatatc ttgattacac tgagatccag cggggacgga
    16261 caaaactctt ctgtattcac taccggcgtc cccggttaaa agcgccgaat gatgagagta
    16321 aggaaaatcc gttgccacct tcacctgcgg aaaaagtcag tccggagatg gcggagaagc
    16381 ttgccctgct tgagaaactg ggcatcacgc tggatgacct ggaaaaactc ttcaaatccc
    16441 gctgaacata aactgtagtc agtgaagatc gctcctttac tgactacagc ttatattaac
    16501 cggggcagtg agtggtttgc tcactgcagt ttatattctg tttcctgcag tgctgtctgt
    16561 agctgagctg ccatctgctg tcccttacgt aagtgatccc gtaacctgat gctgaggcat
    16621 tgctcccttc ataaaacatg acttactcac tacagcttat atacatgctc cagcttatgt
    16681 tatgtctgtt ctgctgacca ctgcttatat aaggaagcgc gtatgccgtc acctcagggg
    16741 aggcagtgta cgcaggatct ccgcagcatc ccgcccgtca cctgtgaaag gcactgccag
    16801 cgtggcagcc atatccagtg caaacactct ggtataaacc tccatagaac gtggatccct
    16861 gtgaccagcc agtgcctgaa tgactttccg gggctggcgg tgataaagca tgtgcatgat
    16921 atagctgtgc cggaaggtgt gtggtgtaac cggaatcgaa aagtgtactc cgtcagcttc
    16981 ggcccgtctg acagcctgct tcagccagtt gcgcatggtt tcgtcggtca cggcccataa
    17041 tggttcacga cgacggggcc gggtggtgat catccagctt tccatctgcc tgacatagct
    17101 tatatctgtc agcggaacta ggcggacttc atcttttggc gggcgtccgc gtcgcgcacg
    17161 cactttttcg gacaggatcc gcacaaacgg tcttgcacca tccagatcaa atgattccgg
    17221 cgtcagcatc cgggcttcgc caatacgcat tccggtattc cagagggtgg cgaacagcat
    17281 atggtgacgc tgatccggca tatagaagag aagggcactc acttccgggg ccagcaggta
    17341 ggccggtgtg gcccccgtgg aagtggccat tcttctcagg gagagcgctg ttgcaaaatc
    17401 cacccccggc gcaatgggta acagggagac gcgttctggt gaattctgca ggggaatgac
    17461 attgttcatg agttattttt cacgtagcac acgcactgtt tatggataca ggtattctga
    17521 cctgaacgcc cggatccttc cacgacaata aatcgcagcg cgcggatcct tttgtttgat
    17581 tatcacgcat tgttgtgggt aaaaccaaat acgtaaaggc gcattttgcc gcaggtatcc
    17641 cgtcttaagc cagtcatggt gcgggttgag agcttgttta ccgtttttgg gagaattggg
    17701 gtaatgccag gagaggcaga catgggtaaa cagagttcag acaggaaaga agtgcaggta
    17761 cgttacgtaa atcctaacca agttttgata tgcgattcga acgcactgca ttatctgctg
    17821 tagaaaatag cttccggata ttcagtagtt ctctgggaat atcccgatgg aaaaatatgc
    17881 agcagataaa tgaatatggc aatagagtgc aatttattca ttacgggatg ctaatctaca
    17941 cataaaatat cagaaaagac tattaataag aataaacaag cccttatatt ataatagtaa
    18001 aaatatggag gtggtatttt ctggatgcaa gttactcgcg ttataaaatt tctttaattt
    18061 tgt
//
LOCUS       DAZFYC010000100        17879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFYC010000100 DAZFYC010000000
VERSION     DAZFYC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.100"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..360
                     /gene="symE"
                     /locus_tag="ACHKW5_003731"
     CDS             19..360
                     /gene="symE"
                     /locus_tag="ACHKW5_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO7513044.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(407..1570)
                     /locus_tag="ACHKW5_003732"
     CDS             complement(407..1570)
                     /locus_tag="ACHKW5_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO7513045.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRCAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPALSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(1618..2500)
                     /locus_tag="ACHKW5_003733"
                     /pseudo
     CDS             complement(1618..2500)
                     /locus_tag="ACHKW5_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2597..2761)
                     /gene="yjiS"
                     /locus_tag="ACHKW5_003734"
     CDS             complement(2597..2761)
                     /gene="yjiS"
                     /locus_tag="ACHKW5_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO7513046.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            2938..4350
                     /gene="yjiR"
                     /locus_tag="ACHKW5_003735"
     CDS             2938..4350
                     /gene="yjiR"
                     /locus_tag="ACHKW5_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7513047.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQILETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSMALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(4593..5549)
                     /locus_tag="ACHKW5_003736"
     CDS             complement(4593..5549)
                     /locus_tag="ACHKW5_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7513048.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRQQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMELIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELIRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKRDPWIEY"
     gene            6016..7248
                     /gene="mdtM"
                     /locus_tag="ACHKW5_003737"
     CDS             6016..7248
                     /gene="mdtM"
                     /locus_tag="ACHKW5_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO7513049.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            7289..8569
                     /gene="yjiN"
                     /locus_tag="ACHKW5_003738"
     CDS             7289..8569
                     /gene="yjiN"
                     /locus_tag="ACHKW5_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO7513050.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            8685..9836
                     /gene="yjiM"
                     /locus_tag="ACHKW5_003739"
     CDS             8685..9836
                     /gene="yjiM"
                     /locus_tag="ACHKW5_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO7513051.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9846..10613
                     /gene="yjiL"
                     /locus_tag="ACHKW5_003740"
     CDS             9846..10613
                     /gene="yjiL"
                     /locus_tag="ACHKW5_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO7513052.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFADAIGAAVIGQRVRTRR"
     gene            10610..10867
                     /gene="ytiA"
                     /locus_tag="ACHKW5_003741"
     CDS             10610..10867
                     /gene="ytiA"
                     /locus_tag="ACHKW5_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO7513053.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            10932..11792
                     /gene="yjiK"
                     /locus_tag="ACHKW5_003742"
     CDS             10932..11792
                     /gene="yjiK"
                     /locus_tag="ACHKW5_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO7513054.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            11860..13038
                     /gene="yjiJ"
                     /locus_tag="ACHKW5_003743"
     CDS             11860..13038
                     /gene="yjiJ"
                     /locus_tag="ACHKW5_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7513055.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMNWWLLAIL
                     YGLAGFGYIIVATFLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13051..13605)
                     /gene="kptA"
                     /locus_tag="ACHKW5_003744"
     CDS             complement(13051..13605)
                     /gene="kptA"
                     /locus_tag="ACHKW5_003744"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO7513056.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            13855..14538
                     /gene="yjiH"
                     /locus_tag="ACHKW5_003745"
     CDS             13855..14538
                     /gene="yjiH"
                     /locus_tag="ACHKW5_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO7513057.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14535..14996
                     /gene="yjiG"
                     /locus_tag="ACHKW5_003746"
     CDS             14535..14996
                     /gene="yjiG"
                     /locus_tag="ACHKW5_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO7513058.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15009..16181
                     /gene="iadA"
                     /locus_tag="ACHKW5_003747"
     CDS             15009..16181
                     /gene="iadA"
                     /locus_tag="ACHKW5_003747"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7513059.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPP
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16246..17157
                     /gene="hypT"
                     /locus_tag="ACHKW5_003748"
                     /gene_synonym="qseD"
     CDS             16246..17157
                     /gene="hypT"
                     /locus_tag="ACHKW5_003748"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO7513060.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(17150..17542)
                     /gene="iraD"
                     /locus_tag="ACHKW5_003749"
     CDS             complement(17150..17542)
                     /gene="iraD"
                     /locus_tag="ACHKW5_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO7513061.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(17539..17622)
                     /gene="idlP"
                     /locus_tag="ACHKW5_003750"
     CDS             complement(17539..17622)
                     /gene="idlP"
                     /locus_tag="ACHKW5_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO7513062.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(17738..>17879)
                     /locus_tag="ACHKW5_003751"
     CDS             complement(17738..>17879)
                     /locus_tag="ACHKW5_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513063.1"
                     /translation="ADYAKINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIFI
                     KS"
BASE COUNT         4139 a         4533 c         4492 g         4715 t
ORIGIN      
        1 ttatagggaa tagcagttat gactgatacg cattctattg cacaaccgtt cgaagcagaa
       61 gtctccccgg caaataaccg tcaattaacc gtgagttatg cgagtcgtta cccggattac
      121 agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg ttttgccacc
      181 ggcacggtgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc ccaaccaccc
      241 gccgccgccg agagcgaact gatgcagtcg ctgcgccagg tgtgcaagct gtcggcgcgt
      301 aaacaaaggc aggtgcagga gtttattggg gtgattgcgg gtaaacagaa agttgcctga
      361 tacattgacc cggtagcgcc aggactaccg ggcatttttc gctgcgttat accaacgcgc
      421 tctccatctg tttttcaaat gcctgacgaa ttaaccccat aatgtaagga agttgtgcga
      481 ggtcactgaa accaatttcc acatcaccat tgccccatcg cccaacattt gtcacatctt
      541 tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac aatcgcagtc
      601 ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca atatagagct
      661 tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataat ttcatcgcga agatgatcga
      721 ataacacccg gctatgcgaa ccatcagcaa gaaaaggata atcactcagg ctgtaactgg
      781 ttttgttttc aggctgcccc cgataaacag ctaaaacctc ttcagaaaga gcaggcgctt
      841 gccagacctg aaccgcgaga tcggccaggc gatcggcacg ggcgcgaatg gcggcttcat
      901 cccatttttc gcactgccca agaccgatat tcaaatagag cgggctatgt ttaaagccgt
      961 cttcaatatc gcgtttttcc gcgaaaggtc tgtcactgta gcgggagtta tagcgcgtta
     1021 aagtgagatt ccccaacgta tgtaacagtt ctttatgcac acgctgccag tcgcttccca
     1081 gctcttcacg ccatttagcc gacagatttt cgttctgcgg cataatgtgc tcaatcgtaa
     1141 actcttccac gcgctctctg cgtttatcgt tttccagtct tcgtaaccag tagctgcggc
     1201 tgcggaagtt atagagatcg cgaactttta attcccgttt aaattcatca tcgttgggga
     1261 aacgacgata tgaaggcagg ttcataaaat gtacctgaat actttccaga tatttttctt
     1321 tattaatgac cttataaaaa gtggcaaacg tcttgttcaa agaattcgtc ggaattgcac
     1381 atactgcaca gcggaaaaca taagattcaa ttaaacgaat tatgctcagg aaatcttcgt
     1441 gagacaaatc gtcattttta tagtcatgat aaagcgccag taagaaagga tacgccacat
     1501 caacctttaa ctcgcgcaaa tcctgaaaag ccgtagcaag cgatttgtca ctttcttttc
     1561 ccaatgccat tgcacagtaa tactccgcgt agatatgaat atcttcaacc agccgatcta
     1621 cgcctttttc agcaacactc tggctgcggg catggagttt aaatgcctca tagacttcat
     1681 ctgtccgagg gatctccccc gtttttaccg tcagataatg acgcataaaa ctgtcaaaat
     1741 attcgctgta accttgctga ccaaaggcga cttccattgg acgtcagtga tcttcataca
     1801 accgggtttg atgctctggt tcgaggccca tcagaataaa attgcgcacc agatcggcct
     1861 gacttaacgc cttaccggtg gagttcatac tttcaaaaat cagttgcgga ttatcctgac
     1921 cacgattaag cgccacatcg acaattaata actttgctaa cccacgacat aaggggatca
     1981 agtcatcacc gagtttggca atttgttcat caaagaaagt aaagttttcc attattcgat
     2041 gggaatagtt ttctggcatt ggtctttgtt ttattaacgc cagtaaactg tctttatcag
     2101 tctcggtcag tagtaattta aaacctttct cgccggactc ataaggattg agcaaataat
     2161 aattacgcaa tttcattgct gaaaagccat caaaaacttc gtcttcgcca agatggcgcg
     2221 ataaagcctc aatcagcaac attgcggtcg tcagccgttg ttgaccatca atgaccagta
     2281 acggagaaat accagaaacc tgatacaacc cctgctcaat ataaacaacc gaaccgataa
     2341 aatgcgctga tatatcgtca cgctttccgg cacgaatgat gtcgtcccaa agttgccgac
     2401 attgttgttc tgtccatgaa taagtccgtt gataaatggg aataacaaac tgctgcgaac
     2461 gttttaaaaa atcgagtagg cgggcttctg tagctttcat tctgacgtct ttccgtgaag
     2521 cgatgatttc gcaataataa ccacttcctt gtggaggtta tagagaaatc ctgtttatcc
     2581 gcgatgaatt gccccctcac tccacatcct ccctgcgtaa cccaatatct ctcaaccgct
     2641 catcactcat ctgctgtaac actcgtcggg tctgcatttg ccgccgccaa cgcctcaccg
     2701 cctgccagag ttgcacaagt ctgataaacg gcgttttggc tttgttttcg tgaaattcca
     2761 tctgctgcct cctcatcttt cagaggcttc atcatccacc ttctggcagt tcacaataca
     2821 gattcaaata ttacttttat ttaacataca gacaggttaa aacagcatct gcatccggtt
     2881 ttttagcctc atctgtaccg gtttttctct ctgtatggtc cacataagga atacagcatg
     2941 acgcgttatc aacatctggc gactctgctt gccgaacgga ttgagcaagg gctgtatcgt
     3001 cacggggaga aattgccgtc ggtgcgcagc ttaagtcagg agcacggcgt cagcatcagt
     3061 accgtgcagc aggcgtatca gatactggag acgatgaagc tcatcactcc gcagccgcgt
     3121 tcgggttatt ttgtcgcaca acgtaaagcc cagccgccag taccgccgat gacgcgtccg
     3181 gtgcagcgcc cggtggaaat tacccagtgg gatcaggtgc tggatatgct ggtggcgcat
     3241 agcgacagtt ccattgttcc gttaagcaaa agcacgccgg atgtcgaaac gcccagcctg
     3301 aaaccgctgt ggcgggagct aagccgggtg gtgcagcata atctacaaac cgttctcggt
     3361 tatgacttgt tagccggtca gcgagtgttg cgagagcaga ttgcccgcct gatgctcgac
     3421 agcggctcgg tggtcaccgc cgatgacatc atcatcacca gcggctgcca taactcgatg
     3481 tcgatggcgt taatggcggt gtgtaaaccg ggcgatattg tcgcggtcga atccccctgt
     3541 tattacggtt caatgcagat gctgcgcggc atgggcgtga aagtgattga aatcccaacc
     3601 gatccagaaa caggcatcag cgttgaagcg ctggaactgg cgctggaaca gtggccgatt
     3661 aaaggcatca ttctggtgcc aaactgtaat aatccgctgg gatttattat gccggacgcg
     3721 cgcaaacggg ccgttctctc tctcgctcaa cgtcatgata ttgtgatttt tgaagatgat
     3781 gtctacggcg aactggcaac ggagtatccg cgcccgcgga ccattcattc ctgggatatc
     3841 gacgggcgag tgctgttgtg cagctcgttc agtaaaagta ttgctccagg cctgcgcgtg
     3901 ggttgggtcg caccggggcg ttatcacgat aaactgatgc atatgaaata cgccatcagt
     3961 agctttaatg tgccgtccac gcaaatggcg gcggcaacgt ttgtgctgga aggtcactat
     4021 catcgccata tccggcggat gcggcagatc tatcagcgca atttggcgct ttatacctgc
     4081 tggatacggg agtattttcc ctgcgaaatc tgtattacgc gcccgaaagg cggattttta
     4141 ctgtggatcg aattgcctga acaggtcgat atggtctgcg tcgcgcggca gctgtaccgc
     4201 atgaaaatcc aggtggcggc aggctcgatt ttctcggctt ccggcaaata ccgtaattgt
     4261 ctgcgtatca actgcgcttt gccgctcagc gaaacctatc gcgaagcact gaagcaaatt
     4321 ggcgaggccg tgtatcaggc aatggaatga attcgaatta gtggcgaggc ccctgatttc
     4381 ctctcacgaa tcgcctgtga ctcgttttat ctttgcaggg gcatcgccac gttccagcat
     4441 ggcttaggca aagtgtgctt tgttcatacc ggatgcggcg tgaacgcctt accaggacta
     4501 caaaatcttt aaagttcaat atattgaatg ggttatgtag acctgataag cgtagcgcat
     4561 aaggctgctt tgcgtttgtc atccgtctct gattagtact cgatccatgg atcacgcttg
     4621 ctttcaggca agggctgatc taatgcctgc atttgctctg ccgaaagtcc ggtatatcgt
     4681 tgtatccatt caggatcggc cccattctgc aacaacaatc ggaggaggct ccgttcccct
     4741 tctactttcc cttctttcct ccccctctcc cttcccaaca gccatccatc attatgaatt
     4801 cgctctgcaa ttgtcattat gcgctccctg tggtgtggca ttcgcctgat tagttcgctg
     4861 ataaacgcct taaaacgcgc ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt
     4921 atctggctgt cattagcaca ctctgtaacc agtaatacta ccagttgatc gataagctcc
     4981 atcagatcgc gctggcgaat atgcttttgg atcaactcca acaaggcgac tctgcgatgc
     5041 tgcacaatct cgtcgtctgg cacgacagtg atatccacca gcgggaacgc tgcagtataa
     5101 agtttccgtg cggtagtcgg gtcggcaaat tcgtccagcc agcacaggga ccaggggtaa
     5161 ggactacggc taccgtgata aaacagcatc gggatgacca gcggtagctg ctggcgttta
     5221 tcatgctcga tatggcgctg catcaccgcc atggaatatc gcatcaggcg aaaggccata
     5281 tggatatcct cgcggctctg atgttcaatc accacataga tataaccatc gccttcgcgg
     5341 gtctttactg accacagaat atcggagtgt agcgcccgca atttttcatc gacaaagctg
     5401 gctgactcca gttttaagct gtcgagatcg cacagttcac gtaaatcttt aggtaaatgg
     5461 atctccataa aatcccgcgc ggtgtcaggg tgcatgagaa aggatttaaa taatgcgtca
     5521 tgcggcgtgc tggtcgtgaa gtttgtcatg gcgatccgtc acctgtagat aagatggccc
     5581 gactttagcg actaacaaaa ccattctcac ctggctattt tccgttcccc gaggcgcttt
     5641 ccagtctatt tttgttcctc tgcaaacgat acatttttcc ggaaacaggc tcgcgtttta
     5701 catttcctgt acccggcaga tattcccctt tccaccgcgc tcttttgatt aaccgcaaat
     5761 tttaattaca cttaaggtgt atattttcta tgcaacccat caattaaaga ggtgtaatgt
     5821 gctgatgact atttgtaatc gttatacatt ctgacctgga gtcagaaggt atttctctgt
     5881 ctgtgtgttc acaggcagtg tggttgatta catgaaattc agtacatttg tccttctcgc
     5941 ctgcctttcg tcattaccga cggtattgaa tttcgttttc cccgttgggg ttctccggac
     6001 aaggagttgt ttgttatgcc acgttttttt gcccgccatg ccgccacgct gtttttcccg
     6061 atggcgttga ttttgtatga ctttgccgcg tacctgtcga cggatctgat ccagccgggg
     6121 atcattaatg tggtccgtga ttttaatgcc gatgtcagtc tggctccggc tgccgtcagt
     6181 ctctatctcg cgggcggtat ggcgttacag tggctgctgg ggccgctttc cgacaggatt
     6241 ggccgcaggc cggtgctgat taccggggcg ctaatattta cccttgcctg cgctgcgaca
     6301 atgttcacca cgtctatgac acagtttctt atcgcgcgtg caattcaggg caccagtatc
     6361 tgttttattg ctaccgttgg ttatgtcacg gtgcaggagg cgttcggaca gacaaaaggg
     6421 atcaagttga tggcgattat cacctccatc gtactgattg cgccgattat tggcccgctt
     6481 tccggcgcag ctctgatgca ctttgtgcac tggaaagtcc tttttgccat cattgcggtt
     6541 atgggtttta tctcatttgt tggcttactg ctggcgatgc cagagacggt gaagcgcggc
     6601 gcggttccat ttagcgccaa aagcgtcttg cgcgattttc gtaatgtctt ttgcaatcgg
     6661 ctgttcctct ttggtgcagc aaccatctct ttaagctata tccccatgat gagttgggtg
     6721 gctgtctcgc cggtgatcct gattgacgca ggcggcttaa cgacctcgca gttcgcctgg
     6781 acacaggttc cggtgttcgg cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt
     6841 aaagatccga ccgaaccgcg ctttatctgg cgtgccgtac ccattcaact ggtcggcctc
     6901 gcactgttga ttatcggcaa tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc
     6961 accagtttgt atgctttcgg gattggtttg atcttcccga ctttattccg ctttacgctt
     7021 ttttccaata acttaccgaa agggaccgtc tccgcatcgc tgaatatggt gatcctgatg
     7081 gtgatgtctg tctcggtcga aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg
     7141 tttcatctgt tagccgtcgt ggcgggcgtt atcgtcgttt tcaccctggc gggattgctc
     7201 aatcgcgtgc gccaacatca ggctgccgag ctggcggagg agcggtgatt tttgcgcgat
     7261 cctgccgtca ggctctattc ttaacgttat gaataaactc attgaactca gacgcgccaa
     7321 aatgttggcg ctctctttac tgcttatcgc cgctgctacc tttgtagtta cgctgttttt
     7381 gccgcccaat ttttgggtga gcggcgtgaa ggcgattgct gaagcggcga tggtcggcgc
     7441 gctggcggac tggtttgcgg tggtggcgct gtttcgccgc gtgccgattc cgatcatttc
     7501 tcgtcatacg gcgattatcc cgcgtaataa agaccggatt ggcgaaaatc tcggtcagtt
     7561 cgtgcaggaa aaatttctcg atacccagtc gctggtggca ttgattcgac gtcacgaacc
     7621 ggcgttgttg attggcaact ggtttagtca gccggagaac gcccgccgcg ttggtcagca
     7681 tctgttgcag atcatgagcg gttttcttga actgaccgat gatgcacgta ttcagcgcct
     7741 gcttaagcgc gcggtccatc gggcgattga taaggtcgat ctttccggaa ccagtgcgtt
     7801 gatgctggag agtatgacca aaaacgatcg tcatcaggtg ctgctcgata cgctgatcgc
     7861 acagttgatc gcccttctcc agcgcgataa atcgcgcaag tttattgccc agcagattgt
     7921 tcgctggctg gagagtgagc atccactgaa agccaaaatt ctccccaccg aatggttggg
     7981 cgaacatagc gcggagttgg tttctgacgc ggtgaattct ttgcttgatg atatcagccg
     8041 tgatcgtgcg catcagatcc gtcatgcgtt tgatcgcgcc acttttgccc tgatcgacaa
     8101 gttgaaaaac gatccggaaa tggcagcgcg agccgatgcc gtaaaaagtt atctgaaaga
     8161 agatgaagct tttaaccgct atctcagtga attgtggggg gatttacggg aatggctgaa
     8221 agcggatatc aacagtgaag attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg
     8281 gtttggcgaa acgttaattg ccgatgatgc cttgcgggca tcgttaaatg gtcacctgga
     8341 acaagccgcg caccgcgtcg cgcctgagtt ttccgcattc ctgacgcgcc acatcagcga
     8401 tacagtaaaa agctgggatg ctcgggatat gtcgcggcaa atcgagttaa atatcggcaa
     8461 agatctgcag tttatccgtg tcaacggtac gctggttggc ggttgtattg ggctaatttt
     8521 atatttgttg tcgcagctcc cggccttgtt ccccctcagc aatttttaga aatccatgaa
     8581 aaactaagtg gctaaccgcg agagagatca aataaaccac actaaaagaa tggtaataat
     8641 gtggcctctt ttcattatct cccgtggtac ggggaaggaa aatcatgtca cttatcaccg
     8701 atctacccgc cattttcgat cagttctctg aagctcgcca gaaaggcttt ctcaccgtca
     8761 tggatctcaa ggagcgcggc attccgctgg ttggcactta ctgcaccttt atgccgcaag
     8821 agatcccgat ggcagccggt gcggttgtgg tttcgctctg ttctacctct gatgaaacca
     8881 ttgaagaagc ggagaaagat ctgccgcgca acctctgccc gctgattaaa agcagctacg
     8941 gctttggcaa aaccgataaa tgcccctact tctacttttc ggatctggtg gtcggtgaaa
     9001 ccacctgcga cggcaaaaag aaaatgtatg aatacatggc ggagtttaag cccgttcatg
     9061 tgatgcagtt gccgaacagc gttaaggacg atgcctcgcg tgcgttatgg aaagccgaga
     9121 tgctgcgctt gcaaaaaacg gtagaagaac gttttgggca cgagattagc gaagatgctc
     9181 tgcgcgatgc cattgcgctg aaaaaccgcg aacgtcgcgc actggctaat ttttatcatc
     9241 ttgggcagtt aaatcctccg gcgcttagcg gcagcgacat tctgaaagtg gtctacggcg
     9301 caaccttccg gttcgataaa gaggcgttga tcaatgaact ggacgcgatg acagcccgcg
     9361 ttcgtcagca gtgggaagaa ggccagcggc tggccctgcg tccacgcatt ttaatcaccg
     9421 gctgcccgat tggcggcgca gcagaaaaag tggtgcgcgc gattgaagag aatggcggct
     9481 gggttgtcgg ttatgaaaac tgcaccgggg cgaaagcgac cgagcaatgc gtggcagaaa
     9541 cgggcgatgt ctacgacgca ctggcggata aatatctggc gattggctgc tcctgtgttt
     9601 cgccgaacga tcagcgcctg caaatgctca gccagatggt ggaagaatat caggtcgatg
     9661 gcgtagttga tgtgattttg caggcgtgcc atacctacgc ggtggaatcg ctggcgatta
     9721 aacgtcatgt gcgtcagcag cacaacattc cttatatcgc tattgaaaca gactactcca
     9781 cctcagatgt cgggcagctc agtacccgtg tcgcggcctt tattgagatg ctgtaaggag
     9841 tggcagtgac atattcgatt ggcattgatt ctggttctac cgccaccaaa gggatcttac
     9901 tggcagacgg cgtgattacg cgccgtttcc tcgttccaac accctttcgt ccggcaacag
     9961 caattactga agcctgggaa actctgcgcg aagggttaga gacaacgccg tttctgacgc
    10021 tcaccggcta tggacggcaa ctggtggatt ttgccgataa acaagtgacg gaaatctcct
    10081 gtcatgggct gggcgcacgg tttcttgcgc cagcaacgcg cgcggtaatc gacatcggtg
    10141 gtcaggacag caaagtgatt cagcttgatg atgacggtaa cctgtgcgat ttcctgatga
    10201 atgacaaatg cgcggcgggc accgggcgtt tcctggaggt gatctcgcgc acgcttggca
    10261 ccagcgtcga gcaactcgac agcattaccg aaaatgtcac gccgcacgcc atcacgagta
    10321 tgtgcacagt gtttgctgaa tcagaagtga tcagcctgcg ctcagcgggc gtcgcgccag
    10381 aagcgattct cgccggagtg attaacgcga tggcgcggag gagtgccaat ttcattgctc
    10441 gtctctcctg tgaagcgccg attctgttta ctggtggcgt tagtcattgc cagacgtttg
    10501 cccggatgct ggaatctcac ctgggaatgc cggtaaatac ccatcctgat gcgcaatttg
    10561 ctgacgcaat tggcgcggcg gtaattggtc aacgagtgag gacacgccga tgaaagagtt
    10621 tttattttta tttcactcca cagtcggcgt catacaaacc cgcaaagcgt tgcaggcagc
    10681 gggcatgact tttcgcgtca gcgatattcc ccgtgattta cgcggcggct gcgggttatg
    10741 catttggctg acctgtcccc ccggcgagga aatacaatgg gtgatccctg ggctgactga
    10801 gtcaatttat tgccagcagg atggtgtctg gcgctgcatt gcacattacc gggtttctcc
    10861 gcgttaagga ttcattaagg tcgctacgta tacttctcga tatcttattg atttgcctta
    10921 aagggattac cgtgacaaaa agtatttcgt taagtaaacg catctccgtt attgtgattc
    10981 tttttgccat tgtggcagtg tgtactttct tcgtgcaatc ttgcgcccgt aaaagcaatc
    11041 atgcagcatc atttcagaat taccacgcca ccattgacgg caaagaaatt gcaggcataa
    11101 cgaataatat ctcttcgctg acctggtcgg cacaaagcaa tactcttttt agcactatca
    11161 acaaacctgc cgccattgtt gaaatgacca ccaatggtga ttttatccgc acaattcctc
    11221 tggattttgt taaggatctc gagacgatcg aatacattgg cgataatcag tttgtcatta
    11281 gcgatgagcg cgattacgcc atttatgtga tttcactgac cccaaactcg gaagtaaaaa
    11341 tcctcaaaaa aataaaaatc ccgctgcaag actccccaac caattgcggt tttgaagggt
    11401 tggcttattc tcgccaggat cacactttct ggttttttaa agagaaaaat ccgattgagg
    11461 tctacaaagt taacgggctg ttaagtagca atgaattaca catcagcaaa gacgaggcac
    11521 tacaacggca atttacttta gatgatgtgt ccggcgcaga atttaaccag caaaaaaata
    11581 cgcttctggt gctgtctcat gaatctcgcg cactccagga agtaaccctg gttggcgagg
    11641 ttatcggtga aatatcacta accaaaggta gtcgaggttt gtcgcataac atcaaacagg
    11701 ccgaagggat tgcgatggat gcctctggta atatttatat cgttagcgaa ccaaatcgtt
    11761 tttatcgctt tacccctaaa tcatcgcatt aacagactgg catatttcac ctttttgtta
    11821 ttaacttgtt aacaacaaaa atgacataca aggttaaaca tgccttcgtc ctcgcatccc
    11881 gtagaacgct tttctttcag caccgcgctt ttcgggatgc tggttctgac cttaggtatg
    11941 ggattaggtc gcttccttta tacgcctatg ttgcccgtca tgatggcgga aggggcgttt
    12001 tcatttagcc agctctcgtg gattgccagc ggcaactatg cggggtatct ggctggcagt
    12061 ctgctatttt cgtttggcgc atttcaccag ccgtcgcgcc tgcgcccatt tctgttggtt
    12121 tctgccctgg cgagcgggtt gttgatcctc gcaatggcat ggttgccgcc atttattctg
    12181 gtgttattga ttcgcttcct ggcgggtgtc gccagcgccg gtatgctgat ttttggttcg
    12241 acgctgatta tgcagcacac gcgccatcct tttgtgctgg cagctttgtt ttctggcgtt
    12301 ggcattggca ttgcactggg caacgaatat gttctggcag gcctgcattt tgccttctcc
    12361 tcgcaaacgt tatggcaagg cgccggggcg ctttctggca tgatgctgat tgcacttacg
    12421 cttttaatgc cctcgaaaaa acacgccatc gcaccaatgc cattggcaaa aacggagcaa
    12481 cagataatga actggtggtt actggctatt ctgtacggcc tggcgggatt tggttatatc
    12541 atcgtcgcga ctttcctgcc gctcatggcg aaagatgcag gctcaccatt gttaaccgcc
    12601 catctctgga cgttagtcgg cttatcaatt gtgccaggct gctttggctg gctatgggca
    12661 gcaaaacgtt ggggggcttt gccttgcctg acggcgaatt tgctggtgca ggctatcagt
    12721 gtgctgctta ctctcgccag cgactctcct cttttgctta tcattagtag tatcggtttt
    12781 ggcggtacct ttatgggcac gacatctctg gtgatgacta tcgcccgcca actgagcgtt
    12841 ccgggaaatc tcaatctttt aggctttgtg acgctcattt acggcattgg acaaatcctc
    12901 ggcccggcgc tgaccagtat gctcggcaat ggaacatcgg cgcttgccgg cgccactctc
    12961 tgcggcgcgg cggcgttgtt tattgctgcg cttatcagca cagtacaatt atttaagctg
    13021 caagtggtca cttcatgagg agtgtttccc ttaccactca agaaattcga ccgcaaccgt
    13081 tgatgtcagc cagactccgt tttccgcttg ccagaaaggg atgcctcgtt tcgccatttc
    13141 ctgtgctttg acggttaaga taaccggcga tccgtgtctt gctcccactt tgcgtgccgt
    13201 tgcttcatca gcagagagat gcacgtaatg acgctcccct gcaatcagcc cctgtttttt
    13261 tatctcgtca aggaaacggc ttgccgtacc gtggtaaaga aactgtggcg gtgttttttc
    13321 ggcaaaggaa atggcgacct gcgaagtcga atgcccctga accgcgcgga tgcacctacc
    13381 atcactggaa taactaaaac gctttttatc actggtagcg accacggtat ccagcaatgc
    13441 acgggttaac cgcttgcccg ctttttgggc acagagtata agcttatcga tatccgccca
    13501 tccttcacgg tcaagtacga taccaatcgc ctccggtttg tgtcggagga caaaacttaa
    13561 aaacttgctc gtatccgcca gctctttttc gttgtacttt gccatctcgt tttcgtccct
    13621 ggattttctg tttttattta ctgatgtcat tgtcactggg tttatgaatt ttctgcaact
    13681 caatcctgcg cagagccttt tccacacagc ataatttttc cgctatgcaa aaaacgcata
    13741 gccagaaaca tacttgcatt ttccccatac ccacatggct gataaggttt ttccgctcca
    13801 ggaattttcc tggctcaagc aacacacaaa cacaaaaaaa caatggggat tgttatgaca
    13861 caacaagggg atgctgtagc cggggagctg gcgacagaaa aagtgggcat taaagggtat
    13921 ctggcttttt ttctcaccat aatctttttt tccggtgttt tttccggaac agacagctgg
    13981 tggcgcgttt ttgatttttc cgttctgaac ggatcgtttg ggcaactgcc tggtgcgaac
    14041 ggagccacca cctcatttcg cggggcgggt ggcgcggggg ctaaagatgg ctttctcttt
    14101 gcgctggagc tggcaccgtc agtcattctc tctctgggga ttatttcaat taccgatggt
    14161 cttggtggat tacgcgccgc gcagcagtta atgacacctg tgctaaaacc gttgttgggc
    14221 attccaggga tctgctcgct ggcgctgatc gctaacctgc aaaacactga tgccgccgcc
    14281 ggtatgacca aagagctggc gcaggaaggt gaaattaccg aacgcgataa agtgattttc
    14341 gccgcatatc aaaccagcgg tagcgccatc atcaccaact acttctcttc cggcgtcgcg
    14401 gtgttcgcct ttctgggcac gtccgtaatc gtacctttag ccgtcatcct ggtgtttaaa
    14461 tttgtcggag ccaacatttt gcgcgtctgg cttaacttcg aagaacgtcg caacccaacg
    14521 caaggagcgc aagcatgaca actcaggtcc gcaaaaacgt catggatatg tttatcgatg
    14581 gcgcgcgccg tggctttact atcgccacca ccaacctgct gccaaacgtg gtgatggcgt
    14641 ttgtcatcat tcaggcgctg aaaattaccg gcctgctcga ctgggtgggg catatctgtg
    14701 aaccggtaat ggcgctgtgg ggattgccag gtgaagcggc aaccgtcctt ttagccgcat
    14761 taatgagtat gggcggcgca gttggtgttg ccgccagtct ggcgaccgct ggcgcattaa
    14821 ccggccacga tgtcaccgtt ttactgcccg cgatgtattt aatgggcaac ccggtgcaaa
    14881 atgtcggtcg ctgcctgggg acagccgaag tgaatgccaa atattacccg catatcatta
    14941 cggtctgcgt gattaacgca ttgctgtcga tctgggttat gcagcttatt gtttaacaag
    15001 gagttaccat gattgattat accgcagccg gttttaccct gctgcaggga gcgcatttgt
    15061 atgcgccgga agatcgggga atttgcgatg tcctcgtcgc taacggcaaa attatcgccg
    15121 ttgccagtaa tatccctcct gacattgtac cggactgcac ggttgtcgat ctcagcgggc
    15181 agatcctctg cccaggtttt attgatcaac acgtccattt gattggcggt ggcggcgaag
    15241 caggtcccac gacgcgcacg ccggaagtgg cgttaagtcg tctgacggaa gcaggcgtca
    15301 cgtcagtggt tggtctgctg ggcaccgact ctatctctcg ccacccggaa tccctgctcg
    15361 ccaagacccg tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc ggcgcttatc
    15421 atgtcccttc ccgcactatt acgggttccg tggaaaaaga cgtggcgatt atcgatcgcg
    15481 tgattggcgt gaaatgcgcc atctctgatc accgttctgc cgcaccggac gtttatcacc
    15541 tggctaatat ggcggcggaa tcccgcgttg gcggtttgct cggcggtaaa cctggcgtca
    15601 ccgtgttcca catgggcgac agcaaaaagg cgttacagcc tgtctatgac ctgctggaaa
    15661 actgcgatgt gccgattagc aagttgctgc cgacccacgt taaccgcaac gttccgttgt
    15721 ttgagcaggc gctggagttc gcgcgcaaag gcggcaccat cgatatcacc agcagcattg
    15781 acgaaccagt cgcccctgcc gaaggtattg cccgcgccgt tcaggcgggt attccgctgg
    15841 cacgcgtcac cctcagctcc gacggcaacg gtagccagcc gttcttcgat gacgaaggga
    15901 atttaaccca tatcggtgtt gccggttttg aaacgttgct ggaaaccgtg caggtgctgg
    15961 tcaaagatta tgatttcagt atcagcgatg ccctgcgccc gctcaccagt agcgtagccg
    16021 gtttccttaa cctgagcggg aaaggcgaaa ttctgccagg caatgacgca gacttactgg
    16081 tcatgacgcc agaattacgc attgagcagg tatacgctcg cggcaagctg atggtcaaag
    16141 acggcaaagc ctgcgtgaaa ggaacgtttg aaacggcata aacggttgtc agccgcccga
    16201 atggacgtag aatgcccgca agggcggctg acagagtaaa acgtaatgga tgactgtggt
    16261 gcggttttgc ataatattga aactaaatgg ctttatgatt ttctgaccct ggaaaaatgc
    16321 cgcaattttt cccaggcagc agtcagtcgc aacgtctcgc aaccggcatt cagccgccgc
    16381 atccgtgcgc tggaacaggc gattggcgtt gaattgttta accgccaggt gacgccgctg
    16441 caactctcgg aacaaggtaa aatattccat tcgcagatcc gccatctgtt gcaacagtta
    16501 gagagcaacc ttgcagaact ccgtggtggc agcgattacg cgcaacgcaa aattaaaata
    16561 gccgctgcac actctctttc cctcgggctg ttaccgtcca ttatcagcca gatgccgccg
    16621 ctctttacct gggcaattga agctattgat gtcgatgaag cggtcgataa actgcgtgaa
    16681 gggcaaagtg actgtatttt ttcctttcac gacgaagatt tgctggaagc gccgtttgac
    16741 cacattcgct tatttgaatc tcaattgttc cccgtttgcg ccagtgacga acacggagaa
    16801 gcacttttta acctcgcgca gccacacttt ccgttactga attacagccg caactcctac
    16861 atggggcgat tgattaatcg caccctgacg cgccacagtg agttaagttt cagcaccttt
    16921 tttgtctctt cgatgagcga gcttttaaag caggttgccc tcgacggctg tgggattgcc
    16981 tggctaccgg aatacgccat ccaacaagaa attcgcagcg ggcagctcgt tgtactgaat
    17041 cgggacgaac tggtgatccc gattcaggcc tatgcatacc ggatgaatac ccgcatgaat
    17101 ccagttgccg aacgcttctg gcgtgaactg cgcgagctgg agattgtgct tagctgacat
    17161 tctccagcgt cgcactgcgc caaccgccat tatcccagac cagatcaaac gttagcgatt
    17221 catcgtcgtg gtacagcatt atctcaaggc tgatcgccag agtgtggcag tacgcttctt
    17281 tcagattaac ctgcaacgct gcaatccgtg gctcatgaaa taacagtgca gaacgcagct
    17341 gacggcaata ccagctctgg agatgtttgt cgctcgggtt atgcacttgc cactcgggca
    17401 tgcctgaaac aagtaatgac ggtaatgccg aatgaggaga attaaaaaga gtgaggagat
    17461 cgctgcatac tgatttacgc agcggtgacg ttttattgcc tgaaatttca ggtaaaacag
    17521 cctgaagtga ttggcgcatc attttgcgca ctcctgacgt ttagcaacat atgcggcttg
    17581 ccctgaacac cgggcaccac tgtattgatg ctcattttcc atagccattc ccttttgttc
    17641 attacatcca tgtcagtctg agacaggcct gaatcctgtc agcaaagtat aaaaccttac
    17701 ttgcctatga atatctactg acttttcagg aaaaaagtca tgatttgata aaaatcatca
    17761 aactcaaaaa aaaaccgcta gtatattctg aatattccga atagttacgt agatgaaaat
    17821 acttgtcacc accgctactt aattccggtg tgaaattatt tattttcgca taatcagcc
//
LOCUS       DAZFYC010000101        17715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFYC010000101 DAZFYC010000000
VERSION     DAZFYC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.101"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            304..1923
                     /locus_tag="ACHKW5_003752"
                     /pseudo
     CDS             304..1923
                     /locus_tag="ACHKW5_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2003..2295
                     /locus_tag="ACHKW5_003753"
                     /pseudo
     CDS             2003..2295
                     /locus_tag="ACHKW5_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2335..3369)
                     /locus_tag="ACHKW5_003754"
     CDS             complement(2335..3369)
                     /locus_tag="ACHKW5_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFO7513064.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQ
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(3372..4337)
                     /locus_tag="ACHKW5_003755"
     CDS             complement(3372..4337)
                     /locus_tag="ACHKW5_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7513065.1"
                     /translation="MKKNKYSTPLWVLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKEIRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            complement(4394..5152)
                     /locus_tag="ACHKW5_003756"
     CDS             complement(4394..5152)
                     /locus_tag="ACHKW5_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFO7513066.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPDHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(5166..6380)
                     /locus_tag="ACHKW5_003757"
                     /pseudo
     CDS             complement(5166..6380)
                     /locus_tag="ACHKW5_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            complement(6576..6936)
                     /locus_tag="ACHKW5_003758"
                     /pseudo
     CDS             complement(6576..6936)
                     /locus_tag="ACHKW5_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6998..7688
                     /locus_tag="ACHKW5_003759"
                     /pseudo
     CDS             6998..7688
                     /locus_tag="ACHKW5_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7876..8376)
                     /locus_tag="ACHKW5_003760"
                     /pseudo
     CDS             complement(7876..8376)
                     /locus_tag="ACHKW5_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            9556..9744
                     /locus_tag="ACHKW5_003761"
                     /pseudo
     CDS             9556..9744
                     /locus_tag="ACHKW5_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            9723..9896
                     /locus_tag="ACHKW5_003762"
                     /pseudo
     CDS             9723..9896
                     /locus_tag="ACHKW5_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            10048..10593
                     /gene="cobU"
                     /locus_tag="ACHKW5_003763"
     CDS             10048..10593
                     /gene="cobU"
                     /locus_tag="ACHKW5_003763"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO7513067.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            10590..11333
                     /gene="cobS"
                     /locus_tag="ACHKW5_003764"
     CDS             10590..11333
                     /gene="cobS"
                     /locus_tag="ACHKW5_003764"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO7513068.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            11345..12424
                     /gene="cobT"
                     /locus_tag="ACHKW5_003765"
     CDS             11345..12424
                     /gene="cobT"
                     /locus_tag="ACHKW5_003765"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO7513069.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            12486..13421
                     /gene="ldtA"
                     /locus_tag="ACHKW5_003766"
     CDS             12486..13421
                     /gene="ldtA"
                     /locus_tag="ACHKW5_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7513070.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(13476..13551)
                     /locus_tag="ACHKW5_003767"
     tRNA            complement(13476..13551)
                     /locus_tag="ACHKW5_003767"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13516..13518),aa:Asn,seq:gtt)
     gene            13878..14795
                     /gene="nac"
                     /locus_tag="ACHKW5_003768"
     CDS             13878..14795
                     /gene="nac"
                     /locus_tag="ACHKW5_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO7513071.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            14897..15847
                     /gene="cbl"
                     /locus_tag="ACHKW5_003769"
     CDS             14897..15847
                     /gene="cbl"
                     /locus_tag="ACHKW5_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO7513072.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(15885..15960)
                     /locus_tag="ACHKW5_003770"
     tRNA            complement(15885..15960)
                     /locus_tag="ACHKW5_003770"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15925..15927),aa:Asn,seq:gtt)
     gene            16121..17608
                     /gene="yeeO"
                     /locus_tag="ACHKW5_003771"
     CDS             16121..17608
                     /gene="yeeO"
                     /locus_tag="ACHKW5_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO7513073.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
BASE COUNT         4589 a         3879 c         4424 g         4823 t
ORIGIN      
        1 actggaagga taggaactga gcggttcagt cgcggaaatc agttgcagag ctgtaatgat
       61 atctccggga gtgctgcgtt ggtaggggca tcccgggata gagaaatttt agcactttgc
      121 cctgaatgat tgtagacgtc ccgagtactc gtaccggtct gagttattac agttagatat
      181 tcactttttc tcccgcatac ttcacatctg tcaacgacgg atgaaaagtg gttcttccgc
      241 aacctgggtg gaagaaaatg gatgaaactt tctggcgaac aaggatctaa gctgttttaa
      301 gttatgggca acgcaatgca ctctcttaag acacttctac agttaccttg cggatggcga
      361 tgcagtcgac aaattattag ctctgacggt ataaccctcc atctccacgg aaaacgcaaa
      421 acagcacaat gtcctgaatg ctttaagcgt agcgactctg ttcatagttg tcgtcggcgc
      481 cggatacagc atctaccctg ctcagggcag acgctatggc ttgtattttc cgtccgccac
      541 tggtactgcc gtaacccggc ttgttcacgt aaaatttttg ccgagtcgct tgctcccttc
      601 gccagttcac accagcagtc ttcacaggcg ttacaaaatt aacaacgtca actgggatta
      661 atagctggag gtgaggctgg aaaacgggct gcaacggcag tgggtctccg ttgcagtgct
      721 gatactcttc ttcgcaggct tatcaatacc ccggagacga aacagtcagg cgcgcctcat
      781 gtcggtattg atgagtgggc gtggcatcgg ggccactgct gcggtatgtt aatagtcaat
      841 cctgatactc accgtcccct cgtcctgctt cccggccgtg atcagcgtac gctggcgacc
      901 tggttcagaa aatatccgga aatacaggtt gtctcgcgtg atcgcagtgg agtctatgcg
      961 acagcagcac gtgaaggtgc acctcaggcc agacaggtgg ccgatcgatg gcacctgcta
     1021 aaaaatattg gtgatgagcc tgaacgaatg atgtacagat atatgcctct gatacgtctt
     1081 gttgtcagag agttatcact gaagaaatca cctgagccag aaatatctgt gcctgtagca
     1141 tcgctccgtc gtctggaacg ccttaaacag cacatccgca aaaaacggca tcagcgttgg
     1201 acagaggtta tggccctgca taacaaggga tgtagtttca gggaaatatc ccgtattaca
     1261 ggcctgtcgc gagtgacagt cagtcgctgg gtgggttcag gaacattccc tgaaatgtca
     1321 actaggcctc caaagcgagg gcttctggac ccatggaggg agtggttaaa agagcaacga
     1381 gaatgtggta attataactc cggccggata tggcgggaaa tggtggccag gggggttaca
     1441 ggcagtgaaa ccatcgtcag ggatgctgtt gccaaatggc ataaaggctg gatcccaccg
     1501 gttactactg ccgcaagact tccttcagtg tcccgggtaa gccgctggtt gatgccctgg
     1561 agaataatca ggggtgaaga aaattatgcg ttccgattta ttagtctgat gtgtgaaaaa
     1621 gaaccggagt tgaaaatagc gcagcaactg gttctcgagt tctaccgtat tctgaaaacc
     1681 taaaataaat cacagctaag cagctggttc actcgggtcc acgaaagcgg ctcagcagaa
     1741 cttcggtgcg tagctgcggg gatggaagct gatgctgcgg ctatatgtga ggcaatcagc
     1801 agtcgctgga gtactggtgt tgtcgaaggt catgtaaatc gcctgaaggt gctgatacgc
     1861 cagatgtatg gtcgagccgg acttgaacta cttaggcgga gggtaatgag tccactggca
     1921 tgaaaatctt cacctaggtt gaggaagagc cacttttgca ctgttcgtga cagtatggcc
     1981 tttatgtact cactttgtta ccggtagctg gatcatcact accgtgccgc aaatggtggt
     2041 gaacagcatc aattgtggaa tgaatgttgc cgaagcgacc aatgcgcctc gtttccacca
     2101 tcagtggttg ccggacgagc tgcgtgttga aaagggttta gtccggatac gctcaagctg
     2161 ctggaagcaa aaggtcagaa agtggcgctg aatgaggcga tgggcagcac gcaaagcatt
     2221 atggttgggt cggatggtga gttgtacggc gcatccgact cgcgcttggt ggatgattta
     2281 acggcggggt attaattgac atcccccggc gcaatgtgcc gggggataat ggttttatgc
     2341 ggtcaataat cgtccgggat tatcgataca gagttttttg aggatcgttt tatcgacgcc
     2401 gcgctccgcc agataggcca gaaaaacatc tggaacaaat ccccagccat taccgccatt
     2461 tttagcccac atttgtttaa ggaagacatc gtggctgaga acaagctgat cggcgtatcc
     2521 caactcaatg agatgagcga cggcatcggc agtctcctgc acccctggcg ctattccctc
     2581 tttcgggaag gtaatgtcta ggccaatcat gtcgaattcc agccagacac ctttatcaag
     2641 cattttccgc tgatacgcca cgtcttttcc tgacggatcc gagtgtgcga gagagacttt
     2701 atttggcgag acgcccattt cacctaacac aatgtcgagt acttcatcac cgcgacgaag
     2761 ccagcccggc atgtgaatat tcatcgccac atgaggattg ttaatctgtg ccagcgaggc
     2821 agcccgcaag ctgttatgct cggcttcggt aaatgtcggt gagacaccta tttcaccgat
     2881 cattccggca cgaatatccg tatcgccaat cccttgattc aattctttat cgatcgttgt
     2941 cgctagttca tcaaccgttt tatgaattct ctggctttca aatttttcca ggtagggacc
     3001 ggaggaagca acaatattca gacctgtttt taatgcgact tctcgtaatg cctgcgcatc
     3061 tcgtccgata gactcagacc ctgtggcatc aacaatggtg cgaccaccga gcgagataaa
     3121 gttattaatt tcgaagatca catcttcaat aggtttgcgg tccatattat cagcgcagca
     3181 ataaggatcg tgttttaatg cccactggat ttctgccgtg acttttttat caacaagctg
     3241 ttgtgaaaaa ggatagcaag gagcatccac aacagaggaa agatcgttga acagatgttc
     3301 atgaggaagt gttagcccca tatcttcacg ggcgacgggg cccgtcacag tttgtagata
     3361 atctttcatg attatacctc cattttcttc attgttttcg aggctctgta ttccggaata
     3421 gtggtaacaa ttgcaccgac cagtgcaaac agtgttccga tgatcgtgac cagataaacg
     3481 gtattgccca gcgatggaat taatacgtca atcaatactg atcccagtaa ctggcccgcc
     3541 gttgaagcaa cccccagcat cagtaacccc agacccctga ccaaaatggc cattaaaccg
     3601 atagacatta gtccaagcgg gccgccaaga tacatccacc agacatctgg taattgaaca
     3661 gtgacgtgcc ctaatgcgac acgaatagct agcgctgtgc ctaaaacaca aaatccaacg
     3721 atgaaattcc aggtgattga aaccagcatg gatcccgtag cttcagctac ttttgcattt
     3781 ccagcgggct gccatcctgc aagcaaacca gctaagaacg gaagaatggc aagtaaaatg
     3841 aaagaggtag aatgccactg cggtgacaca acaaaaatcg tagcaataac cgcaaataat
     3901 gcaccgatga tgcgataagg ggtaaagtat tttttctcgt cgacaccgat accaaagcga
     3961 tcgcaaagta aaccagacag caatagcgcg gagatcagtg cggtctgaaa cgttgcgacc
     4021 ccgagtgcgc tggctgatgc gccttcagaa aataccacca tcgctccaca gagaccggca
     4081 aaccagttcc agagaggaac ttttcggttt ttaatcagag aaggaattgc cgcaaattgt
     4141 tgccggattt cttttcgcgc cataataata aaaaacatca cgactaaacc actggcaaag
     4201 gagataactg cgcatgcatt accgtcctgt aaccagtgac ctaattgacc attcaccgct
     4261 gattgcatag gggagagcat acccgcaaga atcgtggcaa gcacccataa gggagttgag
     4321 tacttattct ttttcataca tcaatccttc tattatagta aatggagtaa ctggtgttac
     4381 cccattatta ttattatctt gcaatatgag tcagtatatt tacccacaac gcggtataga
     4441 acggccagga cataaactgc tgtgtacccc agtgcggtga acagtcactc ataaagcagg
     4501 ctattttccc gttgtgatag ttaccaaaga ccagtaatgg atcgttgttt attgttaata
     4561 ccacctcagc attttcttta gcgatcgccc ggttataacc taggaaaaaa ggatattcag
     4621 agaacccttt aataaccgga tggtcaggtt gtgatggctg tgcaataaca ccctctggtt
     4681 tttcgacacg atcgtcacca tctaacatcg taaccgggag cacatcagcc agaactgtat
     4741 ttttataatt ggcttttgct tcaattccca tgaatgagag atagccgcca atcatcaata
     4801 acccaccacc gttattcaca tattctttaa tcagttcgag agcgtttggt tttatgcgaa
     4861 gctgataaaa agtatcattt tgtagcagga aagtattact accaatatca ctaataacga
     4921 tggcatcata ttgttctaac tgtgccacat cttcagggaa ggcaatttgt accgtatgtg
     4981 ccggcatata ggtgacatct acctgactat ttttcagaca ttggagtaac caggtcgcac
     5041 cttcttcata tttacttgag gtaaaactgt catatccttt tgaatgaatc atatgaatat
     5101 gccaggattc tccaataaag agcaccttta gtgatttttt ggtgttattc attctctgtc
     5161 ccttcttaat gcgtaaaaat agtttgctga taatgtatac tctgaattcg atgtatcaca
     5221 gattcatgtt caggcatgtc ggaagcattg cttgtctcca ccgctaggga tgcaaaggcg
     5281 ctggcataac acagtgctga ttccagtgat tttcctttcg caagtcctga tgccagagcg
     5341 ccgttaaaag catccccggc tcctgccgta tttttaacga ccgctggaaa cgctggcgaa
     5401 tgaatgaatt ttttgccatc aaatgcgagc gagcccttgc taccgagcgt gataacggtg
     5461 ttttttaccc ctttattgtg gatagcttct gcagctcgtt tagctgattc aagatcgtgt
     5521 acgtctatgc cggacagtaa accagcttct gtttcattgg gggtgatata atcaacatcc
     5581 tgaataagtt cgttgacgat atcattatag ggtgccgggt taataatgac cgggatactg
     5641 tttttttgcg ccaatgtgat cgcttgttgc agagcggtat aatttgtttc gagctgcaga
     5701 agtatgacat cagaattaat aatcgcttct ttctgaatag ttatttcatc ggacgacatt
     5761 gtcatgttgg caccgggata gatcgcaata acattatcgc cagtaccttc attaacaagg
     5821 atcgtggcag ttccggtttg cgtttctttc gtttgataaa ttatactttt ataaatctta
     5881 gacgaattca taaagttgat cgcataatca ctaaagtgat ctgtacctac ttttgttatg
     5941 aaataaacat cagtatcggc aaagcttgct gccagcgcct ggttacaacc ttttcccccc
     6001 ggtgaaaata taaaattact tgccagaagc gattctccga tagtgggaag cctcggcaaa
     6061 taactgatga tatcaacatt aaacgaacca ataacgcata cttgactttt catagttgag
     6121 tggccttttt ttattcccta ggctgtatca taattttcaa gcgcataagc gatttcattt
     6181 ttcgcaatag tatcaagcga gtctaggacg ataaaagcac cgccatgata tcgagtcaga
     6241 tagccaagtc tggcaagttc attaagatct gatcgaatag tttctttcgt tacattgagg
     6301 ctattggaca gatcggatac ttttacagaa ccgaatttac tgagtcgttt caatatttca
     6361 tgatggcgtt caaatttcat gcttcacctc acttcagtta cgttaatatc tgtcgatgta
     6421 tattgatgtg ataatcacca taaagtggcg gggaaaagca aaaaaaatca aaaatagccg
     6481 gagaaaaggt aagcgttaat ggagaatcat attgatgctt atttattggt gagaactacg
     6541 ttccatggca ggagttcatc aatacggttg gaaggttatt ccggcagtac gctcaggata
     6601 tggcgcagat actcttccgg atcaatgtcg ttcagaaggc aagtgccgat aagcccatac
     6661 aacagtgcac tgcgctcgcc gtcgaagaac ataaagtttt tatttccgag acagatggca
     6721 caaagggctt tttccgcggc attatgtctg cctccgcctg accgtcaaca ctgtaataac
     6781 agagtgcgcc ccactgattc agtacacagc tgaaccattc tcccagtctg gatttttgga
     6841 cagcgtggca tttttctcca ccatccacgc atgcagtgag tccagtaaca gtttgctctt
     6901 cctctgcctg accacaagat gttctggttc tggcatactg aatttggcca cctgagcata
     6961 ggtaatatgc tcaccttaga acaacacaga tgctccaatg aaaaaaggaa atttcagcgc
     7021 agagtttaaa cgcgaatcca ctcaactagt tgttaaccag aactttacgg tggcaggtac
     7081 catccaaagc tatggatgtc ggtctttcca caatgacaag ataagtcaaa caactgcgta
     7141 atgagcatca gagcaaaaca ccaaaagcct ctctgatgac accagaacaa atcgaaatac
     7201 gtgagctgag gaaaaagcgg caacgtattg aaatagagaa tgaaatatta gaaaaggcta
     7261 cggcgctctt gatgtcagac tccctaaaca gttctcgata atcgagaagc tcagagcgca
     7321 ttatctggtg atagcactct gccatgtgtg caggattcat cgaagcagct acagagattg
     7381 gaaaaaccgt cctgaaaaaa agacggtaga cgggctgtat tacgtagtca ggtaattgag
     7441 cttaatggcg gagcaaggaa catcgccaca attgcaaccc ggagagacta ccagacggga
     7501 cgcaagcttg ctggcagtct catgaaagaa caggggctgg tcagctgtca gccgttgacc
     7561 ctccggtaca ggcatggcag tcatgaactt gtagctatcc taagaacttg aagggcgctt
     7621 agctgtgaca cagctaaatc aagtatggtg acgtgaccta tatctggact ggtaaacgat
     7681 ggggataccc gcttacgcga gagcaataag tggctggcca ccattctcga tacaaaactc
     7741 tgctgaatag cagcgatttt cccagcgtgg tgcgtctttt gcattttact ggttctgagt
     7801 tatcaattat ctgttcacta atgactactc taaaaagttg tatcaattta agtgatatag
     7861 ggagtccgga gattcaccct ttttggtgat gaagttaaca ataccgccaa ttgcctgtga
     7921 accgtacagt acggaatatg gacctttcac tacctcaaca cgctccagcg cagactcatc
     7981 tatcaacagt cccacaccat aattatctcc ggcgcgctga taagttacct cctgaccatc
     8041 aatgagaatt aaaacacggg aggatgcttc gccacgaatg cggatttgtt tacggcctgc
     8101 caaggagttg tctgttatct ctactccggg gatgtcctgc aaattatcgg ctatggaggc
     8161 attggtggag ttctgcaatg tttgttggtc aataacctgt atagtcgccg ggctttccca
     8221 aaggttgctt gccgaacgat tcgtggtcac gacgagcgtc tcttcgttgc tgatttttgt
     8281 ttctgaagaa gttgcggttg cagtagaaac tatcccagaa atgcataatg acagagtgga
     8341 aaggatataa attcgacaag gtatattata taatatttca tccctcatta atacaaagta
     8401 caaatgattg ctgtaatgat aattattttc atttatgttc tcatatatct ataattattg
     8461 tcatcttcat aaattgtaaa tatcaaacat ttttgacctt aatcatgttt tttgatagat
     8521 attttacgac tgaacatatc cgtcgtttcc tctgactcgt gtcagtcggt ctctggttgt
     8581 atccacggac cgcactcttg acataagagt gcggtccgtg gaaaacggag ttgaaaaaat
     8641 ggtgtctgaa agaaggtacc atcattgtga tgacaaatac tttggcgtgg ataatgtaat
     8701 taattgctga aattgaccgt atatgcggta agactcatat cagtatcagg tccggttaat
     8761 tgctgatact aatataaact ttttacaaaa ggatgaatgc agaaggttac tctaatggcc
     8821 agagtgatga caatgatggt cagacgttga cctgcagaaa ctgcatttta attggtctat
     8881 aaagtctgca agaggaatca gggtcagatc agtgagtctt atcgcggccc gaagctatga
     8941 ctgaaagaag gctggataca gaattgcaac aatttggcaa tgtcagaaca cagaagcttt
     9001 gcaagcaggt gaaaggttat gtctagcaaa aggagtatat cagaatatat ctactgatgt
     9061 acaaaacaca acaatagctt catgatatat aaatacgtga tgacgagtgg tactccgcat
     9121 ggttatttaa cttctcagca ccactaatga tcctgagcaa cttcgtattt gggccattac
     9181 catggagtag aaagctgtgt ctgagaatgc agaatttgag aaaagaattc gctttcttgg
     9241 agagcttctg aaaatggcac gctaacaacg ttttgttaaa aaatgtgaat cacttgccgg
     9301 ggctgcattt tctattcgag gagaatgttg acgtagatat tacagaaatc tcagcgcatc
     9361 aggataaact gctaccttag gtcaaccacc aaaagaaaat acaatacgtc cagtttgtat
     9421 gcctttaata acacatcttc taaatgttga agaattatcc cgcctgctga agaacgttgc
     9481 tctgagtgtg ataaaccttt ttattcttga tgatgtcagc gagaagatgg aatatatccc
     9541 cgactgggtc gtggttaccc ggttaaacat ctggtggcgc taataaatct ggcaagtcac
     9601 attctgttga atgctgtgac agtgccttac cgagagagcg aaattgtggt gttctagtgc
     9661 atagtcgcca ccattccgga taattctatt acgttgtttt acaagttctt ttacagcgca
     9721 gatctgtcgc tgacgttgaa tcaatagcct ggatttcaga acacaatgcc cgtttgctgg
     9781 ttgttccggc agtagcatta attcattatc tgaatgaaat gtgtcaatcc acagaaaccg
     9841 ctgtcaatga cgcattttgt cggacgttaa cacgagaaag tctggctatt ccgtaatgcc
     9901 atcttatttc gtaatggatt gagaatgctg gatttttaat tatattacgc gcagtattaa
     9961 tcgcgtaaat gttttgcggt cagcaacggt gggggatcca ttgttctgtt caatgaatac
    10021 catcatacat ttgaatcagg agtcggtatg atgattttgg tgacgggggg cgcacggagc
    10081 gggaagagtc gccacgcaga ggcgcttatt ggggactctt cacaggttct gtatatcgct
    10141 acctcgcaaa tccttgatga tgagatggct gcacggatag aacatcatcg gcaaggccgc
    10201 ccggagcact ggcgcacagt ggagcgctgg caacatcttg atgaattaat tcatgcagac
    10261 attaacccga atgaggttgt gttgcttgaa tgcgttacca caatggtgac taatctgttg
    10321 tttgattatg gcggcgataa agaccctgat gaatgggatt atcaggcgat ggaacaggtg
    10381 attaatgctg agattcagtc gttgattgct gcctgccaac gttgccccgc aaaggttgta
    10441 ttagtgacta acgaagtggg aatggggatt gtgccggaga gtcgtctggc acgacatttt
    10501 cgtgatattg ccgggcgggt aaatcagcag ttggccgctg cggcaaatga agtatggctg
    10561 gtggtttcgg gtattggagt aaaaatcaaa tgagtaaatt attttgggcg atgctctcat
    10621 ttattacgcg tctgcctgta ccgcgtcgct ggtcccaggg actggatttc gagcattatt
    10681 ctcgcggtat tattactttt cctttgattg gattattgct tggcgcgatt agcgggctgg
    10741 tcttcatggt gctgcaggca tggtgtggcg caccactggc ggcactgttt agcgtacttg
    10801 tgctggtgct gatgaccggg ggattccatc tggacggcct cgctgatacc tgtgatggcg
    10861 tattttctgc acgtagccgc gatcgcatgt tggaaatcat gcgtgatagc cgtttaggca
    10921 cccacggcgg tctggcattg atttttgtgg tactggcaaa gattctggtg ttaagcgagt
    10981 tggctctgcg tggcgaaccg atccttgcat cgctggcggc agcatgtgcg gtcagccgcg
    11041 ggactgccgc attattgatg tatcgtcatc gttatgcccg ggaagaaggg cttggcaatg
    11101 tgttcattgg caaaattgat gggcgacaaa cctgcgtcac actcggctta gccgctattt
    11161 ttgctgccgt attattgccg ggtatgcatg gtgtggctgc tatggtggtg acgatggtgg
    11221 cgattttcat cctcggtcag cttctcaaac gtacgctggg cgggcaaacc ggcgatacgc
    11281 tgggagctgc tatagagctt ggtgaactgg tcttcctgct ggcactgcta taaagagacg
    11341 tgacatgcaa acacttgccg atttactgaa tacgatccct gccatcgatc ctgccgcgat
    11401 gtcgcgtgca caacggcata ttgacgggtt actcaaacct gttggtagcc tgggaaggct
    11461 ggaggcgctt gccatacaac tggcaggaat gccggggttg aatggcatac cgcatgtggg
    11521 caaaaaagcg gtactggtta tgtgtgccga tcacggcgtc tgggaggaag gggtcgctat
    11581 ttccccaaaa gaagtgacag ccattcaggc tgaaaatatg acccgcggaa caaccggcgt
    11641 gtgtgtgctg gcagcacaag cgggcgctaa cgtctacgta attgatgttg gtattgatac
    11701 tgctgagcct atccccgggc ttatcaacat gcgtgttgct cgaggtagtg gcaatattgc
    11761 ttcagctccg gcaatgagtc gccatcaggc agaaaagttg cttttggacg tcatatgtta
    11821 tacgcgggag ctggcaaaaa acggtgttac gctgtttggt gtaggtgaac tggggatggc
    11881 aaacacgaca ccggcagcgg caatagtcag cacaatcact ggccgggctc ctgaagaagt
    11941 ggttgggatt ggcgcaaacc tgccgacaga taaactggct aataaaattg atgttgtgcg
    12001 ccgggcgatt acgttgaatc aaccaaatcc gcaggatggc gttgatgtcc tggcaaaagt
    12061 gggtggattt gatttggtcg gaatggctgg cgtgatgtta ggcgctgctt cctgcggttt
    12121 acccgtgttg ctggatggat ttctttctta tgctgccgcg ctcgcagcct gccagatgtc
    12181 tcctgcaatc aaaccgtatc tcattccttc tcacctgtcg gcagaaaaag gggcgcgtat
    12241 tgcgctgtcg catttggggc tggagcctta tctcaatatg gagatgcgtt taggtgaggg
    12301 gagtggcgca gctctggcga tgcccatcat cgaagctgcc tgtgcgatat ataacaacat
    12361 gggcgaactt gctgccagta atattgttct accggggaat acgacttctg atttgaacag
    12421 ttaaaaacga aaagtctgat aaaagctata cttaacggat agctttcgcg acttaggaaa
    12481 gggacatgat gcgtcgtgta aatatttttt gctcatttgc tctgcttttt gccagccata
    12541 ctagcctggc tgtaacttat ccattacctc cagagggtag ccgtttagtg gggcagtcgt
    12601 ttactgtaac tgttcctgat cacaataccc agccgctgga gacttttgcc gcacaatacg
    12661 ggcaagggtt aagtaacatg ctggaagcga acccgggcgc tgatgttttt ttgccgaagt
    12721 ctggctcgca actcaccatt ccgcagcaac tgattttgcc cgccactgtt cgtaaaggga
    12781 ttgtggttaa cgtcgctgag atgcgtcttt attactaccc accagacagt aatactgtgg
    12841 aagtctttcc tattggtatc ggccaggctg ggcgagaaac cccgcgtaac tgggtgacta
    12901 ccgttgaacg taaacaagaa gctccaacct ggacgccaac gccgaacact cggcgcgaat
    12961 atgcgaaacg aggggagagt ttgcccgcat ttgttcctgc gggccccgat aatcccatgg
    13021 ggctgtacgc gatttatatt ggcaggttgt atgccatcca tggtaccaat gccaattttg
    13081 gtattgggct ccgggtaagt cagggctgta ttcgtctgcg caatgacgat atcaaatatc
    13141 tgtttgataa tgttcctgtt gggacgcgtg tgcaaattat cgaccagcca gtaaaataca
    13201 ccactgaacc agatggctcg aactggctgg aagttcatga gccgttgtcg cgcaatcgtg
    13261 cagaatatga gtctgaccga aaagtgccat tgccggtaac cccatctttg cgggcgttta
    13321 tcagcgggca agaagttgat gtaaatcgcg caaatgctgc gttgcaacgt cgatcgggaa
    13381 tgcctgtgca aattagttct ggttcaagac agatgtttta agagcgttgg taattagaaa
    13441 gcaaaagcct gctagaaagc aggctttttt gaatttggct cctctgactg gactcgaacc
    13501 agtgacatac ggattaacag tccgccgttc taccgactga actacagagg aatcgtgtga
    13561 acggggcgca tattaatggc agtgcatggt gatgtcaaag ggtgaaatgc atttttttgt
    13621 ttgtttgctg tcaatttcaa caaagtggtg agctttcaat cttattgggt aatgaaccat
    13681 cgtggtgcat accctccttt tatagggcag gggaacgcga cagctgatta aaggagcaaa
    13741 tgacgaatct atcataatct cttatttgac agttggttag cttgtacatc aacgccaaaa
    13801 taaaactggc aagcatcttg caatctggtt gtaagtaata gcggcacttg ggccgattct
    13861 taaaaaccgg aggcaacatg aacttcagac gcctgaaata cttcgtaaaa attgtagata
    13921 ttggtagcct gacccaggct gctgaagtat tgcatatcgc acaaccagcg ctcagccagc
    13981 aggttgccac actggaaggt gagttaaatc aacaactttt gatccgtaca aagcggggcg
    14041 ttacgccaac agacgccgga aaaattctct atacccatgc gcgggccatt ttacgtcagt
    14101 gtgaacaggc ccaactggcg gtgcataacg ttggtcaggc attatcgggg caagtctcga
    14161 ttggctttgc accaggaacc gctgcgtcat ccatcaccat gcccttatta caggcggttc
    14221 gcgctgaatt tccggagatc gttatctatc ttcatgaaaa tagtggtgca gtgcttaacg
    14281 aaaaattgat aaatcaccaa ctcgatatgg cggtgattta tgagcattcc cctgtggctg
    14341 gtgtatccag tcaggctttg ctgaaagaag atctttttct ggtaggaact caagattgcc
    14401 cggggcaaag cgttgatgtg aatgctattg cgaaaatgaa cctctttctc cccagtgatt
    14461 acagtgctat tagacttcgt gttgatgagg ctttttccct acggcgactc acggcaaaag
    14521 ttattggtga aattgagtct attgccacgc ttaccgcagc gattgccagc ggcatgggcg
    14581 ttgcagtatt acccgaatcg gccgcgcgtt cgttatgtgg cgcagtaaat gggtggatgt
    14641 cacgcattac cactccttcc atgagtctct ctttgtcatt aaatttaccc gccagagcga
    14701 acttatcgcc acaggcacag gcagtgaaag agttgttaat gtcagtgatt agttctccag
    14761 tgatggaaaa aaggcagtgg caattggtga gctaagtgtt atatcgggat ggaataagat
    14821 gcgggttttt attatttgtt atgccgggca ttagacttta acaataacgg gaaatctgaa
    14881 ctgcccggag tttaccgtga atttccaaca actaaagata atccgcgagg ctgcacgtca
    14941 ggattacaac ctgacagagg ttgcgaatat gctttttacc tcacagtcag gcgtcagccg
    15001 tcatattcgg gaactggagg atgaacttgg catcgaaata tttgttcgac gaggtaagcg
    15061 actgctgggc atgactgaac cgggcaaagc attactggtc attgcagaac gtattctgaa
    15121 tgaagccagt aatgttcgtc ggcttgcaga cctgtttacc aacgatacgt ctggcgttct
    15181 cactattgca acgacgcata ctcaggcacg ttatagcttg ccagaggtca ttaaagcttt
    15241 tcgcgaactt ttcccggagg ttcggctcga gctaatccag gggacgccac aggaaattgc
    15301 gacattgttg caaaatggcg aagctgatat tggtatcgcc agcgagcgtt tgagtaatga
    15361 cccgcagctc gtcgccttcc cgtggtttcg ttggcaccat agtttgcttg ttccacacga
    15421 tcatcccttg acgcaaattt caccattgac gctggaatca atagcgaagt ggccgttaat
    15481 cacttaccga caggggatta cggggcgctc acgtattgat gacgcatttg cccgcaaagg
    15541 tttgctggca gatattgtat taagtgcgca ggattctgat gtcattaaaa cctatgttgc
    15601 tcttgggctt gggatcggat tagttgccga acaatccagc ggcgaacaag aggaagagaa
    15661 tttaatccgt ctggatacgc ggcatctttt cgatgctaat actgtctggt tgggactgaa
    15721 gcgaggacaa cttcagcgta actatgtctg gcgctttctg gaactttgta atgcaggact
    15781 gtcagttgag gatatcaagc ggcaggtgat ggaaaacagt gaagaggaaa ttgattatca
    15841 gatatagcaa aaagcccgct ataaaagcgg gcttttcaga aatttggctc ctctgactgg
    15901 actcgaacca gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga
    15961 atcgctgttg aggcacatct taacggcgaa aaatcttttg tcaaacccga tttttaaatt
    16021 ccccaattgt ttgccgtttc tatcaacagt ttgttgcatt tgcagacaat ttgttggcga
    16081 aaatctttgc agctttgctg attctccctc attatttgaa atgtggtttc actttctgca
    16141 attaaggtcg gctttgaata tctcctctgc tttacgccag gttgttcacg gcactcgctg
    16201 gcacgctaaa cgcaagagct acaaagtgtt gttctggcgc gagataaccc cgcttgctgt
    16261 tcctatcttc atggagaatg cctgtgtcct gttgatgggt gtcctgagca cttttctggt
    16321 cagctggctg ggaaaagatg cgatggccgg cgtgggattg gcggacagct tcaatatggt
    16381 cattatggct ttttttgcag ctatcgatct tggtactact gtcgttgtgg catttagtct
    16441 cggtaagcgg gatcgacgac gagcgagggt ggcgacgcgg cagtcattgg tgatcatgac
    16501 gttgtttgcc gtactgttgg caacgcttat tcatcatttt ggcgaacaaa ttattgattt
    16561 cgtcgcgggt gatgccacga cagaagttaa agcactggcg ttgacttatc tggagctgac
    16621 ggtactcagt tatccagcag ctgccatcac tttgattggt agcggggcac ttcgtggtgc
    16681 agggaatacg aaaataccgc tactgattaa cggtagcctg aatattctta atattattat
    16741 tagcggcata ttgatttacg gccttttctc ctggccggga ctgggatttg tcggggctgg
    16801 gttgggttta accatttctc gttatattgg cgcagttgca attttgtggg tgctggcgat
    16861 tggttttaat cctgcgctaa ggatttcgtt aaagagctat tttaaaccgc tgaattttag
    16921 cattatctgg gaagtcatgg ggattggtat tccagcgagt gtcgaatcag tgttatttac
    16981 cagtggtcgg ttattaaccc aaatgttcgt tgccgggatg gggaccagtg ttattgccgg
    17041 aaattttatc gcgttttcaa ttgcggctct tatcaactta cccggaagtg cgctcggctc
    17101 tgcttctacg atcattacag gccgaaggtt gggggtaggg cagatagcgc aagcagagat
    17161 tcagttgcgg catgtgttct ggctgtccac tcttggatta acggccatcg cctggctaac
    17221 ggctcccttt gccgggctta tggcatcgtt ttacacccag gatccacagg ttaaacatgt
    17281 cgttgtgatt ctgatttggc taaatgcttt atttatgcct atttggtccg cctcatgggt
    17341 gctacccgct ggatttaaag gtgctcgtga tgcccgttac gccatgtggg tttcgatgtt
    17401 gagcatgtgg ggttgccggg ttgtcgtcgg ttatgtgctg ggcatcatgc ttggctgggg
    17461 tgtggttggt gtctggatgg gaatgtttgc cgactgggct gtgcgggccg tgctgtttta
    17521 ctggcgaatg gttactggac gttggctatg gaaataccct cgatccgagc cgcaaaagtg
    17581 tgaaaaaaag ccagctgtgt cggaataaac gacaaaatgc agattatttc agcaaacgat
    17641 ttcaaattta aaaaacaggc tttgacatta tgggtgggca tcgctaatat tcgcctcgtt
    17701 ctcacgattc ctctg
//
LOCUS       DAZFYC010000102        17544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFYC010000102 DAZFYC010000000
VERSION     DAZFYC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.102"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(203..2836)
                     /gene="mngB"
                     /locus_tag="ACHKW5_003772"
     CDS             complement(203..2836)
                     /gene="mngB"
                     /locus_tag="ACHKW5_003772"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO7513074.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2854..4768)
                     /gene="mngA"
                     /locus_tag="ACHKW5_003773"
                     /pseudo
     CDS             complement(2854..4768)
                     /gene="mngA"
                     /locus_tag="ACHKW5_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            4937..5659
                     /gene="mngR"
                     /locus_tag="ACHKW5_003774"
     CDS             4937..5659
                     /gene="mngR"
                     /locus_tag="ACHKW5_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO7513075.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5763..6632)
                     /gene="sucD"
                     /locus_tag="ACHKW5_003775"
     CDS             complement(5763..6632)
                     /gene="sucD"
                     /locus_tag="ACHKW5_003775"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO7513076.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6632..7798)
                     /gene="sucC"
                     /locus_tag="ACHKW5_003776"
     CDS             complement(6632..7798)
                     /gene="sucC"
                     /locus_tag="ACHKW5_003776"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO7513077.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7892..9109)
                     /gene="odhB"
                     /locus_tag="ACHKW5_003777"
     CDS             complement(7892..9109)
                     /gene="odhB"
                     /locus_tag="ACHKW5_003777"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO7513078.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQIIGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9124..11925)
                     /gene="sucA"
                     /locus_tag="ACHKW5_003778"
     CDS             complement(9124..11925)
                     /gene="sucA"
                     /locus_tag="ACHKW5_003778"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO7513079.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12126..12842)
                     /gene="sdhB"
                     /locus_tag="ACHKW5_003779"
     CDS             complement(12126..12842)
                     /gene="sdhB"
                     /locus_tag="ACHKW5_003779"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO7513080.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12858..14624)
                     /gene="sdhA"
                     /locus_tag="ACHKW5_003780"
     CDS             complement(12858..14624)
                     /gene="sdhA"
                     /locus_tag="ACHKW5_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO7513081.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQDALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14624..14971)
                     /gene="sdhD"
                     /locus_tag="ACHKW5_003781"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14624..14971)
                     /gene="sdhD"
                     /locus_tag="ACHKW5_003781"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO7513082.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(14965..15369)
                     /gene="sdhC"
                     /locus_tag="ACHKW5_003782"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(14965..15369)
                     /gene="sdhC"
                     /locus_tag="ACHKW5_003782"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7513083.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15750..15857
                     /locus_tag="ACHKW5_003783"
     CDS             15750..15857
                     /locus_tag="ACHKW5_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513084.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16063..17346
                     /gene="gltA"
                     /locus_tag="ACHKW5_003784"
     CDS             16063..17346
                     /gene="gltA"
                     /locus_tag="ACHKW5_003784"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO7513085.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4183 a         4754 c         4351 g         4256 t
ORIGIN      
        1 cacatatacg tggcttgaga gtgaagataa acgcaaaatt acctgatgcg ctacgcttgc
       61 cagtgtctac atcatctctg caatatattg aatttgcctg cttttgtaga ccgaataaag
      121 cgttcatgcc gtatccggca tgaacaaagc gccctttgtc aacaatctgg ccgcgcatat
      181 gtggcttcgg tttgattgct attcaggcaa gccggtaact gaacgtccgt gactggccgg
      241 gtaaaaaagg ccccgataga tttgaacctt gattttcctc ggtggtaatg tgttcatcca
      301 tcatcgtttc tgagcaagaa atcacctcgc gactgaaagc aacagtcgca tcacaggttg
      361 ctgattcagc cggattaaac agccgtaaaa tcacttcttg tcggtcttca gctttcttaa
      421 gtgcgcttat caggcatccc actgggggca ttttcaacaa actataactt tccggcacgt
      481 tgaatccggc tttgttgagc ttcatcgcat cccatgggat tttgttgtaa cactgtactg
      541 gagtcagcca tgctcgcgcc tgctgagcta caccagcggc ggttggcgta ccggtataac
      601 tcaataaact taggcgacaa gaaagcagac cacgtagttg tgagtccggg actggcattt
      661 taattcccga aggccgccca ggccttaaaa acagatcttc tttgcccagt aagcccacgc
      721 cacgcagcaa cgtaatggca aaggttttct tctcttcacc gatgacttca aattcacgta
      781 gcccttcgct aaagacagcc atgccgttac gcccttcctg taaggctgca tagttgagca
      841 tattccatac cggaaccggc gcttctttcc agccttcttg ctgccagttg ttcattgcac
      901 tgtcgttcac ggggcgcgtt agcgaaccaa actgggtatc tgccagaaca ctatcggtgt
      961 taaaagatgt agggatcagg acgcgaaggc gatgatcgtc agcctggtta tcaaggttga
     1021 tatccacatc aatacgtctg ctgttatgac tcagagtgac aaccatctcc acccctactc
     1081 tgcccgtgga ttgccggacg cttcgttcca acaaattgag cggcaccgcc atgtcatagc
     1141 ggataacagc cctgctctgc caggcctcat gagtaatctc gcattgcggt ttcgctgttg
     1201 cagaagtcat tacccactct tcttttgcgg gtgaatagtc atattcatca ccatcatcag
     1261 agctttcttc aatttgcaac acccggtcat agcgcacacc gctatcttta tctaccagtt
     1321 gtagcgttcc atcctcattg agcgcaattt gccagaaagc attttccagt atcccttcag
     1381 cgtcactttt tgctgcaatc acgttgccag gctgattcgc ttcgatataa agcgtgcgat
     1441 agcccataga agggacaatc tggttgatct gtatatcaaa ctccataaag ggatcgtaat
     1501 taccgtaatg aactatttgc cgatcgatta ggcctggatc gatctcacgg gcatggcgaa
     1561 taaaatacgg tacaggctga ccgcgatcgt ctcgtagatt aaactggctg gcgcgcaggc
     1621 gcacagtggt gttaataact tcttcccgcg gccagggcat caggttaaac agcacgagtt
     1681 tgtcggcatc actctgtggc atgttatcgg caattttgcg catgtagaaa cgcagcagat
     1741 tatccgccat gtcttcagcc agttcgaagc gggcgacaat ttcgcgatga actttgtcac
     1801 tgcaacaaca accgatactg tcgtgggcat gattttttaa gatctctttc cacattttct
     1861 ccagcaagcc gtggtggtat tcaaaaccca acgtccaggc cagcgttgcc agcggttcca
     1921 gcagattaac aatcttattt tcaatacgtg cgtgggcaat tttgatatcc atgcgcgtgg
     1981 aaccgatggt gcgatgtacg cgcatatatt tgccatcgat aaattcacct ttcagggtgg
     2041 ccagactttc acgctgcgct tcgatcttct caaatacctc ttcaaagcgg ctcatcacaa
     2101 acttacgttg agggtagatc tcacgtagct tatccatcac ttcgaagata ttttgctgca
     2161 atggcatctg gtcatgccca ttcggcagca aaatctcttt ggttacagac gctttttcca
     2221 gcacgtcaaa ataactgtcg aggcgtttac gtaatccgtt ttcgtcggca ggtaagtact
     2281 taccgatggc gtagcccagc ggcagcacct gcgccgtcac ttcgctaccg tcactgcttt
     2341 gccacaaaaa ctcggtttta tcagtaccgt ggcgctccga acatccgcgc cagaacatgg
     2401 tgcgggtaat gccaaatcca ttgtagatat gcggcagttg cccggacatg ccaaaggaat
     2461 ctggtaaata acctattttc atcggctcgc caaacgcgag gcagtcacgc attccgtaca
     2521 tcagattacg gacgatggat tccgcagaaa caatcgtggt atcggtctgg gtataccagg
     2581 ggccgataat caacttgccg gcttctacct gtttcttcac gcggtctttg ttttccggtt
     2641 tcaccgcgaa ataatcttcg aggatcgccg tttgcccgtc gagtacgtaa tatttgtatt
     2701 cgttgtcctg ttccagtcgg cacaggatct cttccatatt attgaccagc agaatgcgtg
     2761 actcttcggt ggtgaaatac cactcccgat cccaatgcat atgcggggtg atgtgaacgc
     2821 gagataccgc tttcatcgtc gtttcctttt tgtttatggc attacgccat cagtcagata
     2881 gttgccatgc ttaaccgcgt gacgccgcca gatcaggaga attgcagtcg agattgcagc
     2941 cccaccaatg ccgcaccaaa ccagccaatt gccgccataa caccgcccgc gccattatca
     3001 tgaagtaaaa agagcgagaa aatgccggca ccgggtgtcg aaaggccgat attcatcgca
     3061 ccgacaatag cgcccgttac catagagccc agcacaaacg aaccaataac ccgcagcgga
     3121 tcttcaatcg ccatcgggat cgccccttcg gtaatacctg ccagccctaa cagccaggtg
     3181 gatttcccgg tctcaatttc aaactcttta aacaggcgcg gtgcgagcat cgtggaagcg
     3241 gttacggtaa atgccgaaac cattttgacg gaggcgaaaa tggcatacgg gccgtaaacg
     3301 ccgttcgcca ttgcgcccag gcagaatgca taagcggctt tgttcactgg ccctccaagg
     3361 tcaaaggaac acataaaacc gagaatggca cccagcaaca gcgcgttact tcctgacaga
     3421 ccgttcagcc aggcggtaag cgagttattg atccaggcga caggttctcc caccacaaac
     3481 agcatcagac tgcccgctcc caacgtaccg agcaccgggt agagataaaa agtcaggaat
     3541 ccattgaatt tactgctaag acgcaagtga tttttcaccc agcgcatcaa gtaaccggct
     3601 atcaatccgc caacgaccgc gccgagaaac ccggagccga tcatgttggc ggcaagtcca
     3661 gccgcaaagc ctggcgctaa cgccggttta tctgccagag aataggcggt ataggccgcg
     3721 agcaccggta ccatcaaaat tccgagcagc ccgccgccca gcttgcggta catccacagc
     3781 caggagtttt cttcattaaa cagatcttgt agcccgaaga tttgcgacag taataccgcg
     3841 accgccagca ccgtgccccc cgcgacaatc aacggtacgg caaaagagat tccgctcaac
     3901 agtgcctgtt tcagctccgt tttgacactt ttcaccggtt gcgtatcttg ctgtacggta
     3961 cgcgtctcat cgctacgctt gagggtaagc gcttgttgga tcaacgcttc tgcatggcga
     4021 atcggctcgg caacaggcac tgaaagcgcg ggatgccatt aaaacgctca ctctccttga
     4081 tggcgacttc agccgcaaaa atacaggcgg ttgcactatt gagttgatcc gccgttaaac
     4141 gcccttcaat gccgttagcg ccttgttttt caacgtaaac atttacgccg agtttgcgtc
     4201 cggctttttc cagatattcc gcagccatat aggtgtgagc aatacccgcc ggacaggccg
     4261 ttacgcagac gatagttggc gcgttggaaa aagaggcaga aggttgcgtg cctcccttgt
     4321 catccagcgc cgagagcaac tcatcaggcg tcgtcgccga ctgtatacgc gcccgaatct
     4381 catcatccgc caggcgcgtg gtcagcgctg tcagcagttg catatgcgta gtacccgctt
     4441 cattgggggg aatcgccagc agcaccacta aatcaaccgc ttccgggcca tcaacgcctt
     4501 cccactgaag cggctcgctg agtgtggcga ccgcaaacgc cgcttctttt accgcagcag
     4561 ttttgccatg cggcacagcc aacccttcac ctaaggcggt cgggccaagg ctttcacggc
     4621 gatacacttc ttccagaaat tgctcagtac tggaaatttt ccccagagca gcaagacgtt
     4681 gagtcaacgc gtggatggcc tcttcacggc tggtaaagcg cgcattcaga cacaacgcat
     4741 cgcggtgggt cagagtcgtc agattcatca tgatcctcac ttgatcgttt ttatagtcgc
     4801 gccagtgtgc ccgataaaac aataccatta tgtgatcaat ttcacttgat tgaaattatt
     4861 tgtattgtat ttgtattatt tacaggatga atttatcggg cataatggct aaattcgtat
     4921 taatgagata ctaaaaatgg gacacaagcc cttataccgg cagattgccg atcgcattcg
     4981 tgaacaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
     5041 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
     5101 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
     5161 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
     5221 acacagtgaa gtcctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
     5281 acaaatcacc gcgcaggatc gtgtgtggca tgtgaagcgc gtgcgctatc gcaagcaaaa
     5341 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
     5401 ggtcatggaa aattcaaaat atcactttat tgaagaagtg aagaaaatgg ttatcgatcg
     5461 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
     5521 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
     5581 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
     5641 acgaaaatca tcgcgataag cacaaaaaag gccatcatac gatggccaac catgtcgaaa
     5701 ccgacagccc ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat
     5761 atttatttca gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc
     5821 acgcctgcgg cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg
     5881 atggcacccg cgtggcccat acgtttgcct ttcggcgcag tcacaccagc gatgtaaccc
     5941 acaactggct tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg
     6001 ccgatctcac cgatcattac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga
     6061 atgtcgataa agttagagcc agggatcggg tcaccgccga taccaacaca ggtcgactga
     6121 ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa
     6181 acgataccca ctttacccgg tttgtgaatg tgacccggct ggataccgat tttgcattca
     6241 cccggagtga taacgcccgg gcagttcggg ccgatcatac gaacgccggc ttcgtccagt
     6301 ttcactttca cagtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt
     6361 ttgatgcctg catcaatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtag
     6421 ataacagaag cggtagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc
     6481 aggccgaggt gggtagtacc gcctttacct ggggttacgc cgccaaccat tttagtgccg
     6541 tatgcaatgg cctgttctga gtggaaagtc ccctggctac cagtaaagcc ctggcagata
     6601 accttggtgt ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct
     6661 gctgagctgc atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt
     6721 tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac
     6781 ccacttctgc taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc
     6841 cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt
     6901 cggttacacg ttctttggtt gcgccgccgc caacgtcaag gaagttagcc ggttcgccgc
     6961 cgtgcagttt aacgatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac
     7021 cgatgttacc gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg
     7081 gatcttcctg cgactggtca cgcatttcgc gcagatcagg ctggcggaac agtgcgttgc
     7141 cgtcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg
     7201 ggttgatttc gatcaacgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga
     7261 ttttggtgaa ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc
     7321 cctgatacgg catcgggcct gtcagcggat caagcgcaac tttatggatc aggtgcggag
     7381 tttcttccgc cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc
     7441 gacgggaact acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgctg
     7501 cttcaaccag aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac
     7561 gcttgccgag ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca
     7621 caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacgggccgg
     7681 caccgatttt tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg
     7741 gtgctggtaa gccatagcgg gcaaaaagtt gttttgcctg atattcatgt aagttcatgt
     7801 gttctgtcca tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg
     7861 tcggtctaca gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggat
     7921 cttccagcaa ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac
     7981 ggtgatcgta ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg
     8041 ccatcggacg atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga
     8101 tcggcgtaga catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg
     8161 tcagatcttc aacggtcagc ttgccgtcac ggcctttgac tgccagctct ttgattttct
     8221 tctcgatgtc tgccatgccg agggtatcga catcacgcag taccggtgtc accaggccgc
     8281 gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt
     8341 cgatagacgc gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga
     8401 aggacataaa gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac
     8461 gcagatccat aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt
     8521 ttttcgcttc cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt
     8581 tttcactacg tgcagccaga gccggttgcg ccgccggatc agccgctgcc ggagcagact
     8641 ctttcgccgg ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca
     8701 caccggtgcc tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg
     8761 ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag
     8821 tggacgcttt ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca
     8881 ggcgaccaat gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca
     8941 gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca
     9001 gcacttcatc acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat
     9061 cggctacgga ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt
     9121 tatttattcg acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga
     9181 catataccct accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc
     9241 aaacggaatc acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg
     9301 ctcttcctgg caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat
     9361 cgctttatgc gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt
     9421 gttcttacga cgctgttcca gcaggtcgta ataaacctta ccagaacaca ttactacgcg
     9481 cttcacgccc ttcggatcaa gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt
     9541 cgccagttct tcgaggctgg aaaccgccag cggatgacgc agcagggatt tcggcgacat
     9601 cacgaccagc ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg
     9661 tgccggggta gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg
     9721 ttccagacgc gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat
     9781 caccagacca cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac
     9841 cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg
     9901 ttctgcggtg gcataaccgt attcaaacgc cagcactgct tcttcagaca gtacggagtc
     9961 ccaaacacgg aacgcgccct ggccgttatg gatgtgttgc agcggcgtgt aagtggaacc
    10021 gttagactgg ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc
    10081 acccgacagg cgaaccggaa tgccttcgtc aaccagcgtg gcgtaagcga ggttttccgc
    10141 gccgccccag tcgaacagtt tctcaccggc agccatcgcc tggcgatcgc cataaatctt
    10201 ggcaacgcga gactgcattt caaccgcttc cggcaccgtg ctgatgcgtt tagccagttc
    10261 ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta
    10321 cggcgaccag gtgaaagagt gcatgttcat cggacgccac tctgctacca cgcaatcgcc
    10381 agcatccagc gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcgccac
    10441 tttttcctgc tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt
    10501 ctgatacatc agcggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg
    10561 gtagcatacc aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag
    10621 acgggtcaca aaggcaacgg cttccggatc gtccgcgtta acgtggaaaa tcggtgcctg
    10681 aaccatcttg ccgatatcag tacagtacgg cgtagaacgg gcatccagcg gattagaggt
    10741 ggtgaaacca acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg
    10801 cgctttcgac atgttcaggg tttcctgaac cacgccctgt ccggtcactg cggcgtcacc
    10861 gtggatggta attggcagca ctttgttgct gctcggctca tcaagtctgt ccagacgggc
    10921 acgaacagag ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag
    10981 cgccaggtgc accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt
    11041 cacgtcaccc gtgccgaggt gttctttatg tttaccggca aactcgtcga acaagtcttg
    11101 cggtttttta cccagcacgt tcaccaacac gttcagacga ccacggtgcg ccatcccgag
    11161 aaccacttcc cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat
    11221 taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta
    11281 acgttcaagg ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt
    11341 gaaagtcgcg cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt
    11401 aatgtgcata tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cttccagcag
    11461 ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtctcctg
    11521 gaagtctgct tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg
    11581 ccacagtccc agcggatcga gattcgcatg ctggtgacca cggaagcggt atgcgttaat
    11641 gagctgcagg actttaacct gctttacatt ggtgtcaggg tcggagatcg ttgaagagta
    11701 acgtgaagcg tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc
    11761 cggtttgact cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga
    11821 gtcaggatcg gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga
    11881 gaggtaagaa gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc
    11941 atctttttta tgcttacttc gccgtggata ctaccacgca cagttatgtg cgtgccgggg
    12001 ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta
    12061 gtgtcagggg cagtttttaa agatttcctg aatagccggg ggatttcccc ggcatcaaca
    12121 tttaattacg cattacgttg caacaacatc gacttgatat ggccgatggc gcgcgtcggg
    12181 ttcagcccct tcggacatac actgacgcag ttcatgatgc tgtgacagcg gaatacactg
    12241 aatgcatcgc tcaaaccgtc gaggcggctg tcagtctcgg tatcacggct atcgatcagg
    12301 aaacgatatg ccgctaacaa gcctgccggg ccgataaact tatcgggatt ccaccagaaa
    12361 gacggacaag aggttgaaca acatgcgcag agaatacatt catacagccc gtcgagtttt
    12421 tcgcgctgct ctggcatctg taaatgctcg cgagctggcg gattttgtcc attattcaac
    12481 aggtaaggct taattttctc atattgcgca tagaattgtc ccatgtctac caccaaatcg
    12541 cggatcaccg gtaaacctgg cagcgggcga atcacaatct tcttgcccgg ctggttgagt
    12601 gccgaaatcg gggtaataca ggccagacca ttcttgccgt tcatgttcag accgtcggaa
    12661 ccgcacacac cttcacggca ggagcggcgg aacgacaggc tgggatcttt ctctttcagc
    12721 tggataagcg catccagcaa catcatgtcg cgaccttctt ccgcttccag ggtgtaatcc
    12781 tgcatacgcg gagcatcatc aacatccggg ttatagcgat aaattgaaaa ctcgagtctc
    12841 attttcctgt ctccgcatta gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc
    12901 ggttccatgt tgacgcttcg gcgcgtcatg gattccgact ctggcagata cagggagtgg
    12961 cacagccagt tttcatcatc acgatccggg aagtcgaagc ggctatgcgc gccacggctt
    13021 tcggtacgga agttggcaga aacagccgtt gcatacgccg tttccatcag gttatccagt
    13081 tccaggcact caacgcgctg ggtgttgaac tcgctggaag tgtcatccag acgggcattt
    13141 ttcagacgct cgcggataac tttcaactgc tcaagccctt tcgccatcgc atcaccttca
    13201 cggaagaccg agaagttatg ctgcatacat tcttgcagcg ctttacgaat cgccaccgga
    13261 tcttcaccgt tacgattatt gttccagcgg ttcagacgat ccagagatgc ttcaacatca
    13321 gactcgctgg catcgcgcaa tgcgtcctgc tcggcgatag actcttgcag atgcagaccc
    13381 gctgcgcgac caaagaccac caggtccagc agcgagttgc cgcccagacg gttagcgccg
    13441 tgtaccgata cacaagcgat ttcaccaacg gcaaacagcc ccggaacaac cacatcttcg
    13501 cctttctcgt tcaccgtcag cgcctgaccg gtcactttgg tcggaatacc acccatcatg
    13561 tagtgacagg ttgggataac cggaatcggc tctttcaccg gatcaacgtg agcgaaggta
    13621 cgggagagtt ccagaatacc cggcagacgg gattcgagaa cttctttacc caggtggtcg
    13681 agtttcagtt tcgcatgtgg cccccacgga ccatcacagc cgcgaccttc acggatttca
    13741 atcatgatgg aacgcgccac cacgtcacgg cccgccaggt ctttggcgtt cggcgcataa
    13801 cgctccataa agcgttcgcc atgtttgttc agcagataac cgccttcacc acggcaacct
    13861 tcggtgacca gtacgcccgc accggcaata ccggtcgggt ggaactgcca catttccata
    13921 tcctgcaccg gtacgcctgc gcggatagcc atgccgacgc cgtcgccagt gttaatgtgg
    13981 gcgttagtgg tggactgata aatacgccct gccccgccag tcgccagcac ggtagcgcgg
    14041 gctttgaaat aaaccacttc accagtttcg atgcacagtg cagtacaacc caccaccgcg
    14101 ccatcctggt ttttcaccag atccagcgca taccactcgg agaaaatagt ggtgtggttt
    14161 ttcaggttct gctgataaag ggtatgcaac agtgcgtgac cggtacggtc agctgctgcc
    14221 gcagtgcgtg ccgcctgctc gccgccgaag tttttcgact gaccgccaaa cggacgttga
    14281 tagatacgac catcatcaag acgcgagaac ggcaggccca tgtgttcgag ttccagaatc
    14341 gcttccggcc cggttttaca catatattca atcgcgtcct ggtcaccgat atagtccgaa
    14401 ccttttacgg tgtcgtacat atgccattcc cagttatctt catgggtatt acccagcgca
    14461 acggtaatgc cgccttgcgc agaaacggta tgggaacggg tcgggaagac tttagagagc
    14521 agcgcacagg tttgaccgct ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg
    14581 gcaccaatca caactgcatc aaattctctg actggcaatt tcatcacaca ccccacacca
    14641 caacgaatcc ataaatcacg taaaccacca gagcaacgac aatcaccagt tgcagcatca
    14701 ggcgcaaggc cagcggttta acgtagtcgg tcaacacctg ccacatgccg atccaggcat
    14761 ggatcaagat agaaaacagc gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga
    14821 aaccgatcca gacttcatat gtcagctcgc cactggtagc gaaaaaaccg accatataaa
    14881 tgatgtagag cgtcaggacg atagcggtag cgcgaacgag gatgaaatca tgtacgccat
    14941 tgcgtcctaa tgcggaggcg ttgcttacca tacgaggact cctgcgagaa gtgaaagcac
    15001 gacagtaata acaaaggaga ttttggcgga gcgtttaccc gcttcgaatg tttcttccag
    15061 atagccaaaa tccatcatca tgtggcgaat acctacgacg acgtgatacg ccagagcggt
    15121 aaggatgccc cacatgataa atttgacgaa gaagctgccc ataatcgcgg aagcttgctc
    15181 gaaaccttca ggggaagaga ggctggtacc cagaagccac agcaggatac ccactgcaat
    15241 aaaggtgatc acaccggaaa cgcgatggag aatggacgct atcgccgtga tggggaaccg
    15301 gatggtctgt aggtccagat taacaggtct ttgttttttc acatttctta tcatgaataa
    15361 cgcccacatg ctgttcttat tattccctgg ggactacggg cacagaggtt aactttctgt
    15421 tacctggaga cgtcgggatt tccttcctcc ggtctgcttg cgggtcagac agcgtccttt
    15481 ctataactgc gcgtcatgca aaacactgct tccagatgcg aaaacgacac gttacaacgc
    15541 tgggtggctc gggattgcag ggtgttccgg agacctggcg gcagtatagg ccgttcacaa
    15601 aatcattaca attaacctac atatagtttg tcgggtttta tcctgaacag tgatccaggt
    15661 cacgataaca acatttattt aatttttaat catctaattt gacaatcatt caacaaagtt
    15721 gttacaaaca ttaccaggaa aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac
    15781 ctaagattaa cttatgtaac agtgtggaag tattgaccaa ttcattcggg acagttatta
    15841 gtggtagaca agtttaataa ttcggattgc taagtacttg attcgccatt tattcgtcat
    15901 caatggatcc tttacctgca agcgcccaga gctctgtacc caggttttcc cctctttcac
    15961 agagcggcga gccaaataac aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa
    16021 gttccggcag tcttacgtaa taaggcgcta aggagacctt aaatggctga tacaaaagca
    16081 aaactcaccc tcaacgggga cacagctgtt gaactggatg tgctgaaagg cacgctgggt
    16141 caagatgtta ttgatatccg tactctcggt tcaaaaggtg tgttcacctt tgacccaggc
    16201 ttcacttcaa ccgcatcctg cgaatctaaa attactttta ttgatggtga tgaaggtatt
    16261 ttgctgcacc gcggtttccc gatcgatcag ctggcgaccg attctaacta cctggaagtt
    16321 tgttacatcc tgctgaatgg tgaaaaaccg actcaggaac agtatgacga atttaaaact
    16381 acggtgaccc gtcataccat gatccacgag cagattaccc gtctgttcca cgctttccgt
    16441 cgcgactcac atccaatggc agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat
    16501 cacgactcgc tggatgttaa caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg
    16561 tcgaaaatgc cgaccatggc cgcgatgtgt tacaagtatt ccattggtca gccatttgtt
    16621 tatccgcgca acgatctctc ctatgccggt aacttcctga atatgatgtt ctccacgccg
    16681 tgcgaaccgt atgaagttaa tccgattctg gaacgtgcta tggaccgtat tctgatcctg
    16741 cacgctgacc atgaacagaa cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggt
    16801 gcgaacccgt ttgcctgtat cgcagcaggt attgcttcac tgtggggacc tgcgcacggt
    16861 ggtgctaacg aagcggcgct gaaaatgctg gaagaaatta gctccgttaa acacattccg
    16921 gaatttgttc gtcgtgcgaa agataaaaat gattctttcc gcctgatggg cttcggtcac
    16981 cgcgtgtaca aaaattacga cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt
    17041 ctgaaagagc tgggcaccaa agatgacctg ctggaagtgg ctatggagct ggaaaacatc
    17101 gcgctgaacg acccgtactt tatcgagaag aaactgtacc cgaacgtcga tttctactct
    17161 ggtatcatcc tgaaagcgat gggtattccg tcttccatgt tcaccgtcat tttcgcaatg
    17221 gcacgtaccg ttggctggat cgcccactgg agcgaaatgc acagtgacgg tatgaagatt
    17281 gcccgtccgc gtcagctgta tacaggatat gaaaaacgcg actttaaaag cgatatcaag
    17341 cgttaatggt tgattgctaa gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa
    17401 tttttatatg cctaaacaca aaaaattgta aaaataaaat ccactatcgg acattcatat
    17461 attctaaaaa aaatatatcg gtataaatta taaacaaatc gatgattttt aatcatctat
    17521 cttcctctca gttacatgaa taaa
//
LOCUS       DAZFYC010000103        17226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFYC010000103 DAZFYC010000000
VERSION     DAZFYC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.103"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..566
                     /gene="lpxT"
                     /locus_tag="ACHKW5_003785"
     CDS             <1..566
                     /gene="lpxT"
                     /locus_tag="ACHKW5_003785"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO7513086.1"
                     /translation="AFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIM
                     GLVMLLTAVLLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGM
                     MLLIFSAFMWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWV
                     LLTPLSDRLITFFDKSLPGKNKHFQNK"
     gene            978..1544
                     /gene="mepS"
                     /locus_tag="ACHKW5_003786"
     CDS             978..1544
                     /gene="mepS"
                     /locus_tag="ACHKW5_003786"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO7513087.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1725..3281
                     /locus_tag="ACHKW5_003787"
     CDS             1725..3281
                     /locus_tag="ACHKW5_003787"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7513088.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            3363..5177
                     /locus_tag="ACHKW5_003788"
     CDS             3363..5177
                     /locus_tag="ACHKW5_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO7513089.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            5178..6272
                     /locus_tag="ACHKW5_003789"
     CDS             5178..6272
                     /locus_tag="ACHKW5_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO7513090.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMIWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            6272..7297
                     /locus_tag="ACHKW5_003790"
     CDS             6272..7297
                     /locus_tag="ACHKW5_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7513091.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            7299..8888
                     /gene="yejF"
                     /locus_tag="ACHKW5_003791"
     CDS             7299..8888
                     /gene="yejF"
                     /locus_tag="ACHKW5_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO7513092.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(8892..9236)
                     /locus_tag="ACHKW5_003792"
     CDS             complement(8892..9236)
                     /locus_tag="ACHKW5_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO7513093.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9569..10759)
                     /gene="bcr"
                     /locus_tag="ACHKW5_003793"
     CDS             complement(9569..10759)
                     /gene="bcr"
                     /locus_tag="ACHKW5_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO7513094.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(10787..11482)
                     /gene="rsuA"
                     /locus_tag="ACHKW5_003794"
     CDS             complement(10787..11482)
                     /gene="rsuA"
                     /locus_tag="ACHKW5_003794"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO7513095.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11631..13391
                     /locus_tag="ACHKW5_003795"
     CDS             11631..13391
                     /locus_tag="ACHKW5_003795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO7513096.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVSVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            13516..13800
                     /gene="rplY"
                     /locus_tag="ACHKW5_003796"
     CDS             13516..13800
                     /gene="rplY"
                     /locus_tag="ACHKW5_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO7513097.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(13939..14946)
                     /gene="yejK"
                     /locus_tag="ACHKW5_003797"
     CDS             complement(13939..14946)
                     /gene="yejK"
                     /locus_tag="ACHKW5_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO7513098.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            15128..15355
                     /locus_tag="ACHKW5_003798"
     CDS             15128..15355
                     /locus_tag="ACHKW5_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO7513099.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            15375..17135
                     /gene="yejM"
                     /locus_tag="ACHKW5_003799"
     CDS             15375..17135
                     /gene="yejM"
                     /locus_tag="ACHKW5_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO7513100.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
BASE COUNT         4219 a         4413 c         4261 g         4333 t
ORIGIN      
        1 aggccttttt gtggctggtt gcattgacca acaatcgcgc tttcgacggt tgttctctgc
       61 tggcgatggg aatgttgatg ctgagtttct ggctgaaaga aaacgcccct ggcagacgac
      121 gtatcgtgat tatgggtctg gtcatgctat taactgcagt gctattaaac cagctgggtc
      181 aggcattaat tcctgtaaaa cgggccagcc caacattgac ttttaccgat attaaccgcg
      241 tcagcgaact gctctctgtt cccacgaaag atgcctcacg agatagcttt cccggcgatc
      301 acggcatgat gctgcttatt ttttcggcat tcatgtggcg ttatttcggc aaagttgcag
      361 gccttatcgc ccttattatt tttgtggttt ttgcatttcc cagagtaatg attggcgcac
      421 actggtttac tgacatcatt gtcggttcga tgaccgtgat attgatcggt ttgccctggg
      481 tgttgctgac gccattaagt gatcgattaa tcaccttttt tgacaaatca ctaccgggaa
      541 aaaacaaaca tttccaaaac aaataactca cagtaattaa catcatcagg cttattttta
      601 tagtgaggat aatcctgatg atgcgcaccg tgctttcatc catcgcgcgc aaaaatcatt
      661 ctctaagtaa atgaatggat tgcatgcgtt tcactcaatc gtactttaat tgaccaatcc
      721 cgcttattaa ctttctgtat cactttttct tataaaaaat catgtaaaac cgctcgccaa
      781 gaccgcacca atcgggtaat ctcgaactcg ttttgcctcg gcggtagatt atcctcacag
      841 catataattt tgtgcgttag tccacagatt tggccttaag gaattgtttc aacatgccca
      901 ggtaattagt ctcgtgtcgc ttggcatttt tttataacga tatttgtcgt taaggacttc
      961 aagggaaaac aaacaacatg gtcaaatctc aaccgatttt gagatatatc ttgcgcggga
     1021 tccccgcgat tgcagtagcg gttctgcttt ctgcatgtag tgcaaataac accgcaaaga
     1081 atatgcatcc tgagacacgt gcagtgggta gtgaaacatc atcactgcaa gcttctcagg
     1141 atgaatttga aaacctggtt cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg
     1201 ctgactggaa aggcgtacgt tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt
     1261 ctggtttcgt acagcgtaca ttccgtgagc aatttggctt agaacttccg cgttcgactt
     1321 acgaacagca ggaaatgggt aaatctgttt cccgcagtaa tttgcgtacg ggtgatttag
     1381 ttctgttccg tgccggttca acgggacgcc atgtcggtat ttatatcggc aacaatcagt
     1441 ttgtccatgc ttccaccagc agtggtgtta ttatttccag catgaatgaa ccgtactgga
     1501 agaagcgtta caacgaagca cgccgggttc tcagccgcag ctaataaacc gtttggatgc
     1561 aatcccttgg ctatcctaac gagttaactg aaagcactgc ttaggcagtg cttttttgtt
     1621 ttcattcatc agagaaaatg atgtttccgc gtcttgatcc aggctatagt ccggtcattg
     1681 ttatctttta aatgttgtcg taatttcagg aaattaacgg aatcatgttc atacgcgctc
     1741 ccaattctgg acgtaagctc ctgcttacct gcattgttgc aggcgtgatg attgcgatac
     1801 tggtgagttg ccttcagttt ttagtggcct ggcataagca cgaagtcaaa tacgacacac
     1861 tgattaccga cgtacaaaag tatctcgata cctattttgc cgacctgaaa tccactactg
     1921 accggctcca gccgctgacc ttagatacct gccagcaggc taaccccgaa ctgaccgccc
     1981 gcgcggcgtt tagcatgaat gtccgtacgt ttgtgctggt gaaagataaa aaaacattct
     2041 gttcatctgc gaccggtgag atggacattc cactcaatga attgattccg gcgctcgaca
     2101 ttaataaaaa tgtcgatatg gcgatcttac ccggcacgcc gatggtgccg aacaaacccg
     2161 caatcgtcat ctggtatcgc aaccctttgc tgaaaaatag cggcgtcttt gccgctctga
     2221 atctcaacct gacgccttca ctcttttata gttcacggca ggaagattac gatggcgtcg
     2281 ccctcattat tggcaatact gcgctatcta ccttttcttc acgtttgatg aacgttaacg
     2341 aattaaccga catgccagtc cgtgaaacta aaattgcggg cattcctctg accgttcggc
     2401 tttatgcaga tgactggaca tggaacgatg tgtggtacgc atttttactg ggcggcatga
     2461 gtggaactgt cgttggcctg ctctgctatt acctgatgag cgtacgtatg cgccccggca
     2521 gagaaatcat gaccgccatc aagcgcgaac aattttacgt ggcgtatcaa ccggtggtgg
     2581 atacacaagc tttgcgagta acgggcctgg aagtactgct acgctggcgg catcctgtcg
     2641 cgggagaaat tcccccggat gccttcatta actttgccga atcgcaaaag atgattgtgc
     2701 cgctgactca gcatctgttt gagttaattg cccgcgatgc cgcagaatta gaaaaagtgc
     2761 tgccggtagg cgtcaaattt ggcattaaca ttgcgccgga ccatctgcac agcgaaagct
     2821 ttaaagcaga tatccagaaa ctgctcactt ccctacccgc acaccatttc cagattgtgc
     2881 tggaaattac cgagcgcgat atgctgaaag agcaagaagc cacacaactc ttcgcctggc
     2941 tgcactcggt aggcgtagaa attgctattg atgacttcgg caccgggcac agcgcgctta
     3001 tctatcttga gcgttttacg ctcgattatc tgaaaattga ccgtggattt atcaacgcca
     3061 tcggtacgga aacgatcact tcacccgtac ttgacgcggt gctgacgctg gcgaaacgcc
     3121 tcaatatgct gacggttgct gaaggggtcg aaacgccgga acaagcgcga tggctaagcg
     3181 aacgcggcgt taatttcatg caaggctact ggattagtcg cccgttaccg ctggacgatt
     3241 ttgttcgctg gctgaagaaa ccgtatacgc cgcagtggta aggtgtgctt acgtccctta
     3301 ttattcatag tgaaagcatg ccggattgcg gctaatgatg agtaaaagga aatccgttgc
     3361 agatgattgt gcgcatactg ctgctgttta tcgctctgtt cacctttggt gcgcaggcgc
     3421 aggctatcaa ggaaagctat gcctttgccg tgctgggcga accccggtac gcgtttaatt
     3481 tcaaccattt tgattatgtg aaccccgccg cgccaaaagg tgggcagata acgttgtcag
     3541 ccctcggtac cttcgataat ttcaaccgct atgcactgcg cggcaacccg ggcgcacgca
     3601 ccgagcagct gtacgacacg ctatttacga cttccgatga cgaaccaggc agttattacc
     3661 cgctgattgc tgaaagcgca cgctatgctg acgattattc ctgggtggag gtcgctatta
     3721 atccgcgcgc ccgttttcat gatggttcgc ccattactgc ccgcgatgta gagtttactt
     3781 ttcaaaaatt tatgaccgaa ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg
     3841 tcaaagccat tgcgccgtta accgtgcgca ttgagttagc taaacccggc aaagaagata
     3901 tgttgagtct gttttcgctg ccggtatttc cagaaaagta ctggaaggat cacaaactta
     3961 gcgatccgct cgccacgcct ccgcttgcca gtggtccgta ccgaattacg tcctggaaaa
     4021 tggggcaaaa tattgtctat tcccgcgtga aagattactg ggcagcaaac ttaccggtaa
     4081 accgtggacg ctggaatttc gacaccattc gctacgatta ttacctcgat gataatgtcg
     4141 cctttgaagc gtttaaagca ggtgcctttg atttgcgtat ggaaaacgac gctaaaaact
     4201 gggccacgcg ttataccggt aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa
     4261 agaacgaatc agcccaggat acgcgctggc tggcgtttaa tatccaacgt ccggtattca
     4321 gcgatcgccg ggtccgggaa gcaatcaccc tcgcctttga ctttgaatgg atgaacaagg
     4381 cgttgtttta caatgcctgg agtcgcacga acagttattt tcagaatacc gaatacgcgg
     4441 ccagaaatta ccccgacgcc gcggagctgg tgcttctggc accaatgaaa aaagatctac
     4501 cgtcagaagt cttcacacaa atctaccagc cgccggtatc taaaggcgat ggctacgatc
     4561 gtgacaacct gttaaaagcc gacaaacttc tcaacgaagc gggctgggtg ctgaagggtc
     4621 agcaacgcgt taatgccaca acgggtcagc cactcagctt tgaattattg cttcccgcaa
     4681 gcagcaatag tcagtgggta ttgccgttcc agcacagcct gcaacggctg ggtatcaaca
     4741 tggacattcg caaggtggat aactcgcaaa tcaccaaccg catgcgcagt cgcgactatg
     4801 acatgatgcc gcgcgtatgg cgggcgatgc cgtggcccag ttccgattta cagatttcct
     4861 ggtcatcgga atatatcaat tccacttata atgcccccgg cgtgcaaagt ccggttatcg
     4921 actcgctgat caatcaaatt attgccgcgc agggaaataa agaaaaatta ctgccgttag
     4981 ggcgagcact ggatcgcgta ttaacgtgga attattacat gctgccaatg tggtacatgg
     5041 cggaagaccg tctcgcctgg tgggataaat tctcccaacc cgctgtacgc cctgtttaca
     5101 gcctgggtat cgatacctgg tggtatgacg ttaataaagc ggccaaactg ccgtcagcca
     5161 gacaacaggg agagtagatg ggcgcttatc tgattcgccg tctgttgctg gtgatcccaa
     5221 cgttatgggc gattatcact atcaactttt tcatcgtgca aattgcgcct ggcggtccgg
     5281 tcgaccaggc catcgccgcc attgagtttg gtaatgccgg agtattaccc ggcgcaggcg
     5341 gtgaaggtat tcgcgccagc catgcgcaaa cgggcgtcgg caatatcagc gacagtaatt
     5401 accgtggcgg acgcggatta gatccagaag tgatcgccga gatcactcat cgctacggtt
     5461 tcgataagcc gatccacgaa cgttacttca aaatgatctg ggactacatc cgctttgatt
     5521 ttggtgatag cctttttcgc agcgcctcgg tgctgacgct gattaaagac agtctgccgg
     5581 tttccatcac cctcggattg tggagcacgc tgattatcta tctggtgtcg attccgttag
     5641 gcattcgcaa agctgtttat aatgggagcc gctttgacgt ctggagtagc gcatttatca
     5701 tcatcggcta cgccattccg gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg
     5761 gcggcagcta tttcgacctg ttccctctac gcggcctggt ttccgctaac tttgattcgc
     5821 tgccgtggta tcagaaaatc accgattatc tgtggcatat cacgctgccg gtgctggcga
     5881 cagtgattgg tggctttgcg gcgctgacca tgctgaccaa aaactcattc cttgatgaag
     5941 tgcgcaagca atacgtggtg accgcgcgtg cgaaaggggt aagtgaaaaa aatattctct
     6001 ggaaacatgt gttccgcaac gccatgctgc tggtgattgc cggttttccg gcgacgttta
     6061 tcagcatgtt ttttaccggc tcgctgctga ttgaagtgat gttttcactc aatggccttg
     6121 gcttactggg ctacgaagcg accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt
     6181 atattttcac cctgattggc ctgctgctga atattgtcag tgatatcagc tatacgctgg
     6241 tcgatccgcg tatagatttt gagggacgtt aatgtcgcga ctcagccccg tcaatcaggc
     6301 ccgttgggcg cgttttcgcc ataaccgtcg cggctactgg tcgttatgga ttttcctcgt
     6361 tttgtttggt ttgagtttgt gttctgaact tatcgccaac gataaaccgt tgctggtacg
     6421 ttatgacggc agttggtatt tcccgttgtt gaaaaactac agcgaaagcg attttggcgg
     6481 cccgctggca agtcaggctg attatcagga cccgtggctg aaacaacggc tggaaaataa
     6541 cggctgggta ctgtgggcac cgattcgctt tggtgctacc agtatcaact ttgctaccga
     6601 taagcccttc ccttctcccc cctcccggca aaactggctg ggaacggatg ccaacggcgg
     6661 cgatgtgctg gcacgcattc tctatggcac gcggatctcg gttctgtttg gcctgatgct
     6721 gactctctgt tccagcgtga tgggcgtgct ggcgggggcg ctacaaggct attacggcgg
     6781 taaagtcgat ctctggggac aacgctttat tgaagtatgg tcggggatgc cgacgctgtt
     6841 tttgattatt ttactttcca gcgtcgtaca gcctaacttc tggtggctgc tggcaattac
     6901 tgtcttgttt ggctggatga gtctggtcgg cgtggtgcgg gcggagtttt tacgtactcg
     6961 taatttcgac tacattcgtg cggcacaggc gcttggcgtc agcgatcgca gtatcatcct
     7021 gcgtcatatg ttgcctaatg ccatggtcgc gaccctcacc tttttaccgt ttattttatg
     7081 tagttcgata accaccctga cctcgctcga tttcctcggc ttcggtctac cgctcggttc
     7141 accgtcactc ggtgaactgc tgttacaagg gaaaaataac cttcaggccc cgtggcttgg
     7201 gatcaccgcc ttcttgtcgg tggcgatatt attgtctttg ctgatcttta ttggtgaagc
     7261 cgtccgcgac gcatttgatc ctaataaggc ggtgtagcat gacgcaaact ctgttagcga
     7321 ttgaaaattt gtcggtgggt tttcgccatc agcaaaccgt acgtacagta gtcaatgatg
     7381 tttcactaca gattgaggct ggcgaaacgc tggcgctggt gggtgagtca ggttcaggca
     7441 aaagcgttac cgcgctgtca attttacgcc tgctcccttc cccgccggtt gaatatctct
     7501 ccggcgatat tcgttttcat ggcgaatcgc tgcttcatgt cagcgatcaa acgttgcgcg
     7561 gtgtacgcgg taataagatc gccatgattt ttcaggaacc gatggtgtcg ttaaatccat
     7621 tgcataccct ggaaaaacag ctttatgaag tgctttcact ccaccgcggg atgcgtcggg
     7681 aagcggctcg tggcgaaatt cttaactgcc ttgatcgcgt tggtatccgc caggcggcaa
     7741 aacggctgac agattatccg catcagctct ccggcggcga acgccagcgg gtgatgattg
     7801 cgatggcgct gttaacgcga ccggaattat taattgccga tgaaccgacc accgcactgg
     7861 acgtctctgt ccaggcgcag attttacagc tgttgcgcga actgcaaggc gagctgaata
     7921 tgggcatgct gtttattact cataacctca gcattgtcag aaaactggcc caccgcgtgg
     7981 cggtaatgca aaacggtcgc tgtgtcgagc aaaattacgc cgctacgcta tttgcatcac
     8041 ccactcatcc ttacacacaa aagctactca acagtgaacc gtcaggcgat ccagtgccgt
     8101 tgccagaacc tgcctcaacg ttgctggatg ttgaacagct tcaggttgcc ttccccattc
     8161 gcaaagggat tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac atcagtttta
     8221 cgctacgagc gggtgaaaca ctgggtttag tgggcgagtc cggttccggg aaaagtacga
     8281 cgggactggc gctgctgcga ctgattaatt ctcagggcag catcatcttt gacggtcagc
     8341 cactgcaaaa tttaaatcgc cgccagctgt tacctattcg tcatcgcatt caggtggtat
     8401 ttcaggatcc aaactcctcg ctcaacccac gactcaacgt tttgcagatt attgaggaag
     8461 gcttacgggt tcaccagccg acgctttctg ccgcacaacg cgaacaacaa gtgatagccg
     8521 tgatgcatga agtgggatta gatcctgaaa cacgccaccg ttatccggcg gagttctctg
     8581 gtggtcagcg acaacgtatt gcgattgcca gggcattaat tcttaagccc tcgctgatca
     8641 tacttgatga accgacatca tcactcgaca aaacggtaca ggcgcaaata ttgacgctat
     8701 tgaaatcatt gcaacaaaag catcaactgg cctatttgtt tatcagccac gatttgcacg
     8761 ttgtccgcgc gttatgtcat caggttatcg tactgcgaca aggggaagta gtggaacaag
     8821 gaccgtgcgc gcgcgtgttt gccacaccgc agcaggagta tacgcgtcag ctactggcgt
     8881 tgagctgacg cttaaaaagg attgtagttt gaaaagggtt cggcaattgc cacaccaaaa
     8941 tttttcaatc ggcatgttgc agcaaactcg tcctggcgat ttacaaacag gcacggctca
     9001 ccttcacact ccagcaccga acatggaact tcgatgtcgc ttaacgcctg gcttagttta
     9061 tacatcaccg accatgcatt atcaccatcc gggctaagca atttaagcgc caccaggctg
     9121 ttgtcaccgc acggtccatt ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca
     9181 gaccagcgat agttctgcgg cagtcgatgg acgattgaga gttgtaatga agtcacttat
     9241 tttccccgga agtacattca cttcacaatt tgtttacatc aattttaaca catcatcaac
     9301 aatccgtctt tatacagatc gtaaagattg atgcctcgcc tgcgtggcct ctatggctct
     9361 gatttaagta atatctgcgg gcgtggtcac gtttgcgggg ctatatgtac ccgtttctgc
     9421 gatctaactc aaccttttta actacaatga tgtgactttt tacataaatt gattttacat
     9481 aaaataaaca tatatcgggg gaatgataga tttgtggttg acatgcatca acaaaggaag
     9541 ccttttagct tcctcgttgt gcaatagatc accgtttttt cggtcgactg gcgtacagac
     9601 agaagagaat ggagctgctt gcgcagaatg caattgacca aatcatcggc catgcagagt
     9661 taaaggtcgc aagagaaagc aatgcgccaa caattgcccc tatgccaaaa cggaaggttc
     9721 ctgccagcga agatgccgtt cccgccatat ggggaaactc atcaagaatg accgccatcg
     9781 cattggatga caccatcgac acgcagccca caaacgccgc aacgccaacc accagcgacc
     9841 agaatcccag ccccagcagc gcactgatga ccatccacgc tgccataata aattgtatcc
     9901 acaaccccga gcggaacata tttaacgcgc caatgcggcg aacgaagcgg ctgttaaaaa
     9961 tggtcatcac gaacaggaaa acaatgttta gtgcaaagta ataaccaaag ttttccggcg
    10021 cgacgtggtt aatttcaatg tagacaaacg gtccggcgct taagaacgag aacattccgg
    10081 caaagctgaa accgctggcc agcatataac tcaggacacg cttatgacgg aacagcgccg
    10141 caaagttacc aatagtggta cgaatgtgaa atggctgacg acgctccggt ggtaaggttt
    10201 ctttaatcag gaagaaaatc attgccgaag ccagaatcgc cgctaatgcc aggatccaga
    10261 agatgtaatg ccagctcagc cacaccagca cccaaccgcc gacaatcggt gccatcaacg
    10321 gtgcaatagt ggtcaccagc atgacaaacg acatcatccg cgagaactct tctttcgggt
    10381 aaatatcgcg catcaaagcg ttgataacca cgctggccgc agccgccgcc agcccgtgga
    10441 agaaacgcat cacaatcagc tgatcgatgg tgtttgccaa cgcacacgcc accgcggcgg
    10501 cggcaaacac cagcgtaccg ccgagcacca ccggcttacg cccgaagctg tctgccatcg
    10561 gcccgtagat taactgcccc aacgcaaagc ccagaatata agtactgagg gtcatctgcg
    10621 tactgcccgc aggtacgcca aactgcgctg aaattaccgg tagcgcgggc agatacatat
    10681 caatcgacag cggcatcaac atggccagca ggccaaggat aaaaacaata gcaaacgacg
    10741 aatgctgtcg ggtggtcaca acgggctcct gaaagtcatg gaaaagttag acgacgctgg
    10801 caatttcttc ttcagttaac ggacgatatt caccgggggc taaatcagca tccagcgtaa
    10861 taccgccaat acgttcacga tgcagctcaa ccacgtggtt acccacggcg gcgaacatgc
    10921 gtttcacctg atgataacgc ccttcgctga tggtcagacg aacctgcgtt ggggtaatca
    10981 cttccagcac cgcaggctta gtgagatctt tttcgttatg cagctgcacg cctttagcaa
    11041 attgctctgc cgtatcgtca gctacaggtg attccagtgt caccagatag gtcttctcgc
    11101 aatgatggcg cggagaagta atgcggtgcg accactgacc atcatcagtc atcagcacca
    11161 gaccggtggt atcgatatcc agacgtcctg cggcgtgcag tttccaggct accggttcat
    11221 ccagaaaata gagcaccgtt gggtgatcag ggtcgtccgt ggagcaaaca tagccctgag
    11281 gcttattgag catgaagtaa cgtggaccat gttgctgcgc cagcgggttg ccatcgtaag
    11341 cgacatcatg ttcaggaagc agtttgaacg ctgcattacg aacaatttcg ccatcgacgg
    11401 tgacacgatt gccgcggatt tcacgcccgg caatagcacg gctaacgccg agttgctgtg
    11461 cgataaattt atcaagtcgc atgtgtgtga ttttgcctgt aaaaacggag gtcgggcatt
    11521 gacccgaaaa tctgaactgt tgtctgccca gtatagcggt ctaataacat ccctcaaggg
    11581 aaaaagatcc atggcatact attagcagaa taatctacct acgcgagacc atgattttta
    11641 cactccgccc atatcagcaa gaagccgtgg atgccacgct caaccatttt cgtcgtcata
    11701 aaacccctgc cgttatcgtg ctgcccaccg gcgcaggtaa aagcctggtg attgccgaac
    11761 tggcacggct ggcgcgtggt cgtgttctgg tgctggcaca cgtcaaagaa ctggtggcgc
    11821 aaaaccatgc aaagtatcag gcgctggggc tggaagccga tatttttgcc gctgggttaa
    11881 agcgcaaaga gagccacggt aaagtggtat ttggcagcgt gcagtcggtc gctcgtaatc
    11941 ttgatgcctt tcagggtgaa ttttcgctgt tgattgtcga tgaatgccac cgtattggtg
    12001 acgatgaaga gagccagtat cagcaaatcc tcactcacct gactaaagtg aatccccact
    12061 tacgcctgct ggggctgact gccacgcctt ttcgattggg caaaggctgg atctaccagt
    12121 tccattatca cggcatggta cgcggcgatg agaaagccct tttccgtgac tgcatttatg
    12181 agctgccgct gcgttatatg attaaacacg gctatctgac gccgccagaa cgactggata
    12241 tgccagtagt gcaatacgat ttcagccgct tgcaggcaca gagtaacggg ctgttcagcg
    12301 aagctgatct caaccgtgag ctgaaaaaac agcaacgcat tacgccacat atcatcagcc
    12361 agatcatgga gttcgccgaa aagcgcaaag gggtaatgat tttcgccgcc accgttgaac
    12421 acgcaaaaga gattgtggga ttactgcctg ccgaagatgc tgcactgatt actggcgaca
    12481 cccccggcgc tgagcgcgat gtgttaattg aagattttaa agcccagcgt tttcgctatc
    12541 tggtcaacgt ctcagtactg accaccggat ttgacgcccc gcacgtcgat cttatcgcca
    12601 ttctgcgccc taccgaatca gtgagtcttt accaacaaat tgtcgggcgc ggtctgcgtc
    12661 tcgctccggg caagactgat tgcttaattc ttgattatgc gggtaatcct cacgatctct
    12721 acgcgccgga agttggtaca ccaaaaggca aaagtgacaa cgttccggta caggttttct
    12781 gccctgcctg cggttttgcc aacacctttt gggggaaaac gaccgccgac gggacattga
    12841 ttgaacactt tggtcgtcgc tgtcagggat ggtttgaaga tgacgacggt catcgcgaac
    12901 aatgtgactt ccgtttccgt tttaaaaatt gcccgcaatg taacgcggaa aacgatattg
    12961 ccgctcgccg ctgccgcgaa tgtgacaccg tactggttga tccagacgat atgttaaaag
    13021 cggcgctacg actgaaagac gcgctggtat tacgctgtag cggcatgtct ttacagcatg
    13081 ggcatgacga gaaaggcgaa tggttgaaaa tcacctatta cgatgaagac ggcgcggatg
    13141 tgagtgagcg tttccgtctg caaacgcccg cccagcgaac tgccttcgag cagcttttta
    13201 tccgcccgca tacgcgcaca ccgggcatcc ctctgcgctg gatcaccgcc gccgatatcc
    13261 tcgcccagca agccttattg cgacacccgg attttgtcgt tgcccgcatg aaaggtcagt
    13321 actggcaagt gcgtgaaaaa gtgttcgatt acgaaggtcg ttttcgtcgg gcgcacgaat
    13381 tacgcggtta atggtagttt tgattgatgt ataaagcgat tgagtataga atctcgcccg
    13441 cttttgcata cgcaaagcag atcacttacc tgttgctggg tcgcctgtag caggattaat
    13501 ttaagagaga aagaaatgtt tactatcaac gcagaagtac gtaaagagca gggtaagggt
    13561 gcgagccgcc gcctgcgtgc cgctaacaag ttcccggcaa tcatctacgg tggcaaagaa
    13621 gcgccgctgg ctatcgagct ggatcacgac aaagtcatga acatgcaagc taaagctgaa
    13681 ttctacagcg aagttctgac catcgttgtt gacggtaaag aaatcaaagt taaagctcag
    13741 gacgtacagc gtcacccgta caaaccgaag ctgcagcaca tcgacttcgt tcgcgcttaa
    13801 ttgctgaata agttgtaaaa aaccccgctc cggcggggtt ttttgtatct gcagattatg
    13861 cctgattacg gtattgctat tttttgcagg ccagataagg cgttcacgcc gcatctggca
    13921 ttcagaacac aaaaccgatt aattgccgcc agacgtccgg cgctgcaatt ggtcacgcaa
    13981 attcggcggc gtgcctttaa tggtcagggt atcggttgcc ggatcccaga agatgcgttc
    14041 acctaacagc atcgcatcaa agttaatagt caagccaccg ccgctaccgg caaacttcgt
    14101 caactgacgc agtgtgctac ggtctgccgg gaagctctct tccagttcgt agcctttttc
    14161 agccgcaaac tccgtgaaac tcacttcgct gacgcctgcc agctctttcg acagcgactc
    14221 cagttcgatc tcttcgcctg cctgcagctg ctcattacag tagctataaa cctgctggcg
    14281 cacgttctgt cgctctgctt tatccagttg tgcttcggca gtgaaatcat caaccgcttg
    14341 caacagccca cggttttgcg ctttcgcgtt taacccttcg ctggccccga ggaaatccat
    14401 aaagaaatcc gccactttgc gccctactcg ccctttcagg aaagtgagat agcgggtgga
    14461 ctctggattg gtttcccatt cggttaaatc gatacgcgca acaatatccg catgattgat
    14521 atcaagataa tgggttggat tgatgtcgag attttcgtta acgcgcatac tgctcaggtt
    14581 gctcagaact gccaccagca gatactccac cgccagataa cggtagtggc aaaacagcac
    14641 gaatccaccg tcagcaaaag gatatttcgc taattcgtca cgcaggcgcc cggttgccgc
    14701 gcggctaaat gccagaaagt cctcttcgcc ctgacgctgt aagcgcagcg tctgcgccag
    14761 ttcactctct tcgctaaaca agccgtaagc tttatttttg gcgctataga cccgatgcag
    14821 ttctgccacc atctcaacga cggtttctgt cggttccagc aatgaatcgc gcaacaccag
    14881 ctcaaggttc tgctcatcac gcttgataag ctggtgcaga gcaatctggt tgatatccag
    14941 actcatgata aactctcctt taagaccggg cggtattcaa ccaccgccgg ttaagcgacg
    15001 caataaaaga tggcggtcat tttacagaag gcagcgtaac gagaatgatt taacgcataa
    15061 aaaagcagaa aaaaaaccgt tgctacggta atatgttgcc ctttcatcaa ctaactgatt
    15121 tcgatttatg ccacaaattt cccgctacag tgatgaacag gttgaacaat tgctcgcaga
    15181 gctgctcaac gtactggaaa aacataaggc tccgaccgac ctttccctga tggtgttagg
    15241 caacatggtc actaacctta tcaacaccag cattgccccg gcccaacgcc aggcaattgc
    15301 caactctttt gcccgcgcct tacagtcctc tatcaacgaa gacaaagcgc actaagggaa
    15361 acagataaca ggttatggta actcatcgtc agcgctaccg tgaaaaagtc tcccagatgg
    15421 tcagttgggg gcactggttt gcactgttca atattctgct ttcgctcgtc attggcagcc
    15481 gttacctgtt tatcgccgac tggccgacaa cgcttgctgg tcgcatttat tcctacgtaa
    15541 gcattatcgg ccatttcagc ttcctggtgt tcgccaccta cttgctgatc ctcttcccgc
    15601 tgacctttat cgtcggctcc cagaggctga tgaggttttt gtccgtcatt ctggcaacgg
    15661 cgggaatgac gctattactg atcgatagcg aagtctttac tcgtttccat ctccatctta
    15721 atcccatcgt ctggcaactg gttatcaacc cagacgaaaa tgagatggcg cgcgactggc
    15781 agctgatgtt catcagcgtg ccggttattt tattgcttga actggtgttt gcgacgtgga
    15841 gctggcaaaa gctgcgcagc ctgacgcgtc gtcgacgctt cgcgcgcccg ctggccgcat
    15901 tcttatttat cgcctttatc gcctcgcatg tggtgtatat ctgggccgat gccaacttct
    15961 atcgcccgat caccatgcag cgcgctaacc tgccgctttc gtacccgatg acggcgcgac
    16021 gttttcttga gaagcatggt ctgcttgatg cgcaggagta tcaacgccgt cttattgagc
    16081 aaggtaatcc agacgccgtt tccgttcagt atccgttaag cgaactgcgc tatcgcgata
    16141 tgggcaccgg gcagaatgtg ctgttgatta ctgtcgatgg cctgaactac tcacgcttcg
    16201 agaagcagat gcctgcgctg gcaggttttg ctgagcaaaa tatttcgttc acgcgccata
    16261 tgagctccgg caacactaca gacaacggca tctttggcct gttctatggc atctcgccga
    16321 gctatatgga cggcattctg tcgacccgta cgcctgcggc attaattact gcgcttaatc
    16381 agcaaggcta tcagctgggg ttattctcat cagatggctt taccagcccg ctgtatcgcc
    16441 aggcattgtt gtcagatttc tcgatgccga gcgtacgcac ccaatccgac gagcagaccg
    16501 ccacgcagtg gatcaactgg ctgggccgct acgcacaaga agataaccgc tggttctcgt
    16561 gggtctcttt caatggcact aacattgacg acagcaatca gcaggcattt gcacggaaat
    16621 atagccgggc ggcaggcaat gtcgatgacc agatcaaccg cgtgctcaat gcactgcgtg
    16681 attctggcaa actggacaat acggtggtga ttatcactgc cggtcggggt attccgctga
    16741 gcgaagacga agaaaccttt gactggtccc acggtcatct gcaggtgcca ttagtgattc
    16801 actggccagg cacgccggcg cagcgtatta atgctctgac tgatcatacc gatctgatga
    16861 cgacgctgat gcaacgcctg ctacatgtca gcacacctgc cagcgaatat tcgcaaggtc
    16921 aggatttgtt caaccctcaa cgccgtcatt actgggttac tgcagcggat aacgatacgc
    16981 tggcaattac caccccgaaa aagacgctgg tgctgaacaa taacggtaaa taccgcactt
    17041 acaacttacg tggtgaaaga gtgaaagatg aaaaaccaca gttaagtttg ttattgcaag
    17101 tgctgacaga cgagaagcgt tttatcgcta actgattaat tataaatcag ttagcaaaat
    17161 atcttacttg caatcggtgt ggaaaacggt agtattagca gccacgagtc ggcacgtagc
    17221 gcagcc
//
LOCUS       DAZFYC010000104        17184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFYC010000104 DAZFYC010000000
VERSION     DAZFYC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.104"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..730
                     /gene="ais"
                     /locus_tag="ACHKW5_003800"
     CDS             128..730
                     /gene="ais"
                     /locus_tag="ACHKW5_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO7513101.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKW5_003801"
     CDS             complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKW5_003801"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO7513102.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEIMPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVAARKTLRLKGLL"
     gene            1473..2015
                     /gene="yfaZ"
                     /locus_tag="ACHKW5_003802"
     CDS             1473..2015
                     /gene="yfaZ"
                     /locus_tag="ACHKW5_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO7513103.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     GGNRDNAVADGPYVGVNASF"
     gene            2115..3317
                     /gene="yfaY"
                     /locus_tag="ACHKW5_003803"
     CDS             2115..3317
                     /gene="yfaY"
                     /locus_tag="ACHKW5_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO7513104.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3381..3842
                     /locus_tag="ACHKW5_003804"
                     /pseudo
     CDS             3381..3842
                     /locus_tag="ACHKW5_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3883..4785)
                     /gene="rpnE"
                     /locus_tag="ACHKW5_003805"
     CDS             complement(3883..4785)
                     /gene="rpnE"
                     /locus_tag="ACHKW5_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO7513105.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4979..6169)
                     /gene="glpC"
                     /locus_tag="ACHKW5_003806"
     CDS             complement(4979..6169)
                     /gene="glpC"
                     /locus_tag="ACHKW5_003806"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO7513106.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6166..7425)
                     /gene="glpB"
                     /locus_tag="ACHKW5_003807"
     CDS             complement(6166..7425)
                     /gene="glpB"
                     /locus_tag="ACHKW5_003807"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO7513107.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7415..9043)
                     /gene="glpA"
                     /locus_tag="ACHKW5_003808"
     CDS             complement(7415..9043)
                     /gene="glpA"
                     /locus_tag="ACHKW5_003808"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO7513108.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVAAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9316..10674
                     /gene="glpT"
                     /locus_tag="ACHKW5_003809"
     CDS             9316..10674
                     /gene="glpT"
                     /locus_tag="ACHKW5_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO7513109.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10679..11755
                     /gene="glpQ"
                     /locus_tag="ACHKW5_003810"
     CDS             10679..11755
                     /gene="glpQ"
                     /locus_tag="ACHKW5_003810"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7513110.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11797..12036)
                     /locus_tag="ACHKW5_003811"
                     /pseudo
     CDS             complement(11797..12036)
                     /locus_tag="ACHKW5_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12034..12138
                     /locus_tag="ACHKW5_003812"
                     /pseudo
     CDS             12034..12138
                     /locus_tag="ACHKW5_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12218..12868
                     /gene="inaA"
                     /locus_tag="ACHKW5_003813"
     CDS             12218..12868
                     /gene="inaA"
                     /locus_tag="ACHKW5_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO7513111.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12922..13176)
                     /gene="yfaE"
                     /locus_tag="ACHKW5_003814"
     CDS             complement(12922..13176)
                     /gene="yfaE"
                     /locus_tag="ACHKW5_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO7513112.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13176..14306)
                     /gene="nrdB"
                     /locus_tag="ACHKW5_003815"
     CDS             complement(13176..14306)
                     /gene="nrdB"
                     /locus_tag="ACHKW5_003815"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7513113.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14453..16738)
                     /gene="nrdA"
                     /locus_tag="ACHKW5_003816"
     CDS             complement(14453..16738)
                     /gene="nrdA"
                     /locus_tag="ACHKW5_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7513114.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYSKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16866..17009
                     /gene="ypaB"
                     /locus_tag="ACHKW5_003817"
     CDS             16866..17009
                     /gene="ypaB"
                     /locus_tag="ACHKW5_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO7513115.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4057 a         4196 c         4596 g         4335 t
ORIGIN      
        1 aaaattttga ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt
       61 atcgccagaa atgtcgctca attttacggt aattgtctgg ttgcgcttgt ctataggtgg
      121 agtttacgtg ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt
      181 actggcgctc gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt
      241 gccacgtatc gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt
      301 gtttcgccat gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg
      361 tattacggtt aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat
      421 ccctgatttc gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt
      481 ttccgcgggt aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta
      541 tagtgcaatt aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca
      601 taatcattgc ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta
      661 tctggatggt ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt
      721 taaccactaa attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc
      781 tttcaaacgt aacgtttttc gggcggcaac atttaaatca taatgcacca gatcttcagg
      841 ttttacccac gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac
      901 gcagtcaaaa atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt
      961 ggtgcgaata tcatcgctga aggtccacgg catgatttct gtcaaaagca gctgttctcc
     1021 cagttcttcg cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc
     1081 gccacccgaa agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag
     1141 caaataagca ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcataggggt
     1201 attccttata aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga
     1261 cagtaatctc ttttgcaatg ataactgcac ttattattat ttttgtgtgt atcggtaaag
     1321 tgtgtttatt ttttcggcgt ttaattattt aagaagtatt tagttacaat aagtgactag
     1381 atcccttttt tcgctcattg aatcctctgt gccaggcagt acaatcccgc agctttggga
     1441 attttgatta attgcttgtg ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg
     1501 ccggaatgct gttggtttct gcatcggtca atgcaatgag catcagcggc caggcgggta
     1561 aagaatacac caatattggt gtcggttttg ggactgaatc gacgggcctg gctttaagcg
     1621 gtaactggac acataacgac gacgacggtg acgtcgcggg cgtggggctg gggttgaatc
     1681 tgcctctcgg gccgttaatg gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt
     1741 acggcgatga aggttatgcc gcagcggtag gaggtggttt gcagtggaaa attggcaaca
     1801 gcttccgttt gtttggcgag tattactact ctccggattc gctctccagc ggtattcaaa
     1861 gttatgagga agcgaatgct ggcgcgcgtt acaccattat gcgtccagtc agtattgagg
     1921 cgggttatcg ctacctgaat ctgtcgggta aaggcggtaa ccgcgacaac gccgtggctg
     1981 acggcccgta tgttggggtt aacgccagtt tctgatccct tccggcgcgg cattctgtcg
     2041 cgccattcag tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa
     2101 agcaggagat aaccatgtta aaagtggaaa tgctatccac cggggatgaa gtgttacacg
     2161 ggcaaatcgt tgacactaac gctgcctggc tggccgattt tttctttcat caggggctgc
     2221 cattatctcg ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg
     2281 aacgtagtca gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg
     2341 atttaagcgc actcgccgct gcgacagcaa aaggtgaagg catggtgctg catgaagcct
     2401 ggctcaaaga gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc
     2461 gtaaacaagc ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct
     2521 gtggttttgc cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag
     2581 aatttaaggt gatggtcgag cacgaaatcc tgccgcgtct gcgcgagcgt ttttctttac
     2641 cacagccgcc ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac
     2701 aaagcctgga cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcttcaatgc
     2761 cgattatcga actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt
     2821 ggctggacgt taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc
     2881 ccgcgcagat cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat
     2941 tcaccggcgg tttattggct ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg
     3001 aagtggttcc ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc
     3061 gggccaacca ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca
     3121 actttgcgct agccacgcca gacggcactt tcgctctgcg tgtgcgtttc agcactacac
     3181 gctacagcct ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctgcgcc
     3241 gttggttaaa cggccaggat atcgccagtg agcatggctg gattgaggtt attgagtcca
     3301 tgaccttatc tgtctgattc cgccactctg tgccggttta ctgcaccggc acattccccc
     3361 tccggcaaaa cttgtccaac cctccctacg gtgagcgtgg tgttggggcc agtgtggcac
     3421 gggctgcgcg ctggggacgc attgagaatt acatggcgca agttaacgat tcactttgtc
     3481 tgttggtgca ggtggaaagt aaaacggcac tggataacct ggacgaaatc ctcgacgtcg
     3541 aagggattga tggcgtgttt attggacctg cggatctttc tgcgtcgttg ggctacccgg
     3601 ataacgcagg gcacccggaa gtgcagcgaa ttattgaaac cagtattcgg cggatccgtg
     3661 ctgcgggtaa agcggctggt tttctggctg tggctcctga tatggcgcag caatgcctgg
     3721 cgtggggagc gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg
     3781 atcaacgact ggcgatgttt aaatcaggca aaaatgggcc acgcataaaa ggtagttatt
     3841 gatatcaaag gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tattcgctgc
     3901 cagatcggct tcgcttagct gggtggccgc gagcacctgg tcacgatcaa tgccctgttc
     3961 cagcatgcgc aaagcaattt taatggcttg ttcatgcata ccttcctgcc agccaatttg
     4021 atgcccttcc tgccgtagcc gttcagcaat agtcattaat ctctcctttt gtagtggtga
     4081 acgttcggca atctcctcaa taaaacgggt gaaacgggag gtatcgccgc attgcagcag
     4141 ataattaaac agtgtttgta gctggctgtc attagtgaag cctctaacca aaagcgtggt
     4201 gatcctgtcg accatgccga ttaaatcgcg gtcgcgaata tgcttttgaa tcagttccag
     4261 cagagctata cgccgatgtt gcatgatctc atcgtcaggt acgatggtga tatccaccaa
     4321 cggaaaagct tcggtgtaca actgccgggc aagttgcgga tcgtcaaact catccagcca
     4381 gttaagtgag tacgggtagg gcgatgtttc gccatgataa aacagcaatg gcaccaccag
     4441 cggaactctg tcatagcctt tatccaggtg acgctgcatg gcggcagtgg catagcgcat
     4501 tagccgaaaa gccatattct tttctgcaga gctttgatgt tcaatcacgc agtagatata
     4561 gccgtcaccg tcgctggttt ccacggacca caaaacatcc gagtagtaag cgcgtaaact
     4621 tttttcaata aaactagtgg gttccaggcg taaggtttgc aggttgcaaa gcttgcgcag
     4681 tggttctggt aaatgtattt cgagaaaatc ccgtgcggtt tcgggtgtga acataaaggt
     4741 tttaaacacc gcatcatgcg gggaggatgt tgttgattcg gtcatggttg tccgtcacct
     4801 gatatttatg atgacgcgac attacgtcta tcccgggcct ttgacatctg tcagtttcag
     4861 cctttgcgag acattttcag aggaattttc aaagcgttgc aacgcataca tggtgcgtgg
     4921 aatcgcgctc gcaaaaatgc ccgataagca atgtgcttac cgggcatcaa aaggtcggtt
     4981 aagccagcgc ctgggccagt agcgtaatcg gatgttcgca gcgaaggctc gtggacatct
     5041 caatctgcca tttgcaggtt tcgcagtcgg tgatgaccag atccgcgccg ctctcttcta
     5101 tctggcggaa cagtggcgca ccgatggctt gtgaggtggg gtagttctct tttttgaaac
     5161 cgtaagtacc cgcaataccg cagcactggg aatccagcac cgttaactca agccccggga
     5221 ttttacgcaa cagctccagg gtgtagagcg tccagcccat tttttccata tggcacggag
     5281 tgtgataaac cactttcagc ggcagcggtt tcagcggtaa cgttttgcct tcgtccagct
     5341 tgcgccacag ccagcgggtt gccagttcga tatgatcgcg caagcctttg ttgtcgacat
     5401 tcagcacttc cgggtattcg tcgcgcaggg caaatgtaca ggttgaggag gtggcaatca
     5461 ccggaattcc ttttactccc acagcttcgc ggatcgactc tacattcgta attgcctgtt
     5521 tgcgtgcttt atcggtaaag ccgttggcga ttagcggtac gccgcagcat ttttctttgc
     5581 tgagcagttg tacaccggta cccattgcgt tgagcacttt aattaaatct ttacctaact
     5641 gcggatggtt gtagttaacg aagcagccgt gaaagaaagc gacctggtct ttatattgtg
     5701 cttgctgagc cgctatgctg cgataccagc gacggaacgt gccgaaggag tatttcggta
     5761 gcgtgcggcg atgatcgatt tttaacgccg catcaagcag ctgccgcacc ggtttcagcg
     5821 aggtagcggt gttgacgatt ggtgcgaacg gcgtggaaac gctacccatc aggtcggtat
     5881 gactcaacac aaaattacgc agcgacgggc gcgtggtgtc atattttgcc cgcgcgcgct
     5941 ggataatatc gccaatcttc acatcggacg ggcaggcgac ttcacaacgt ttgcagttga
     6001 tgcaatattt cagcgcctcg tcatacagtg cgccatcttt caaacgcaga cgctcgccat
     6061 ccggcccggc ttgttttggc cctggataac cgggattcac ccggctcacc gggcaggcgg
     6121 tggtgcagac ggtgcactta atgcagtttt cgaagctggt gtcgttcatt gttggcctcc
     6181 tgcgcgttgg gcaatctgtt gagcggcatg taaagcactg acggcacaaa caccgccgcc
     6241 gcatccctgg gcgatgggat caaatccgcc cagcaccgaa ccgatggcaa acaggttctc
     6301 aatggtttgc cctgcctgcg acgggcgtag cgtctcatca gtggttacac cgaactgctg
     6361 ccacggttgc ggcgcaaaaa aatcgccctt ataccattca ccccgcgtgg cggtttgtag
     6421 cacatcaagg ccgagaatcg gctcgcgaat gccgttacgt tccgctacca gtccgccact
     6481 aaagaaactg ccgctggcga gaaccgcgaa acgtggacgt agcggaatat cggcgtgatt
     6541 gcgggtccag atttcattca ctacgccatt tttacaggtc acttttttca cttcatcgcc
     6601 cggcatccac acgccaccct ggcgcacaaa ctggcgctgt aactggtttt gcagacgaat
     6661 gcccagcacg gaaggcggca gcgttggcaa aagcatcagt gaacaaggta gtttttcatt
     6721 caaccaacgc cacagtttgt catcggccag gccgaagcag gcgggcatca ggatcatttc
     6781 gcaggtattg gcgacaggaa taagcgcatc aagtaacagc ggccagtttt cttcattatc
     6841 aaggaaacgg gcgatattca cagcgcgaaa ttcggtggcg ttattgcgca ggacatccag
     6901 ttccggcagc tctatttctg cggtttcaac cgaaagatcc agttcgcgca acgaagccgc
     6961 cgcaagatgc gcctggaaat ccatcagccc gcttatcccc actacacata tcttcttcgc
     7021 gggtagcggc cagacgggaa cttctggcga acttaaccag gttgcacgca aagtgccgag
     7081 cggggtaacg cgctgatggg ccagttccac gctgccctgc aattgcgcac cgctttcagc
     7141 gatcaatgcc tgcgcctggc aagcgagatc gagcacgcgt tgtggttcga gaagactgta
     7201 aggatgggct ggtgcctgct gacgcaaaga ttccagtcca ctgtgaatgt ctgtcaccgg
     7261 ttgaccatca ggcagatggc tcagcaaatc cagcgaaccg gatgagaaat gcagcgcgct
     7321 ttgaccacgc gtgacaatgg cacagcgcag gccgtgtttt tgcagttgca ggccacagag
     7381 taatccggcg aggccgccgc ccataatgac agtatcaaag cgcatctttc tgctccttct
     7441 ccagaccaca cagtccctga taaacccagc gggtaaattc gctttcgcgc agtgcgtctc
     7501 cccaggcgat gggttgcacg cctttccagc gttcgttaag gaaggtggaa agttgctcga
     7561 tagattgcgc ggacgtcgtg acgttaaaac gttgcagcag tccggcagcg cggcaggcgc
     7621 agagttcgcc ctggcaggta cccatcccca cacgggtacg acggcgtaaa tccagcaggc
     7681 tattaacgtt taaattttct accgcgtact gaacttcacc cgcagttacc gcttcgcact
     7741 cacataccag gctacgatgc agacggcctt cgctcagcca ggcaggcgtg cgatcgccat
     7801 gacgataaac cgcagaaccg cgcagcgggg caggcaggga gatgacttta cgcaaggtaa
     7861 cttcagcggg ttcttgtgaa ccaggcagtg ccagatcggc agtcgtacag gggcgcgtgt
     7921 tgcccagttt gcggcatacc gcgtcggtag cccattcagc catcagccga taggtcatca
     7981 gtttgccacc ggtgatggtg ataaatccgt ccagaccatc gcgttcagca tggtcgagca
     8041 gcacgatgcc acggctgacg ttacgtccgc tcgggtcgtc atcgctggca accagcgggc
     8101 gcacgccaga ataggcccgc aaaatgcgcg ttttcgccat cacgggggcc agtttttccc
     8161 cttcacgcag cagaatatca acctcttctg ccgccactcg attatcgtca atctcgttgt
     8221 aatcaatacg taaagaggtg gtgccaatca gcgaaatggt atcgccaggc accagaatat
     8281 cggcgtcgga aggtttacgg cagcggttga tcacatgctg gttaatgcgg tgatccatga
     8341 tcagcagcga tcctttcgcc gggaacatgc gaatgcgcag atcggcatat tcggcaatgt
     8401 gttgccccca gatcccagcg gcattaacca cgacaggtgc atgaagggcc tgagtttcgc
     8461 cggtgagatg gttacgtaca cgaacaccgc acaccgtcgc gccttcacga atcagccccg
     8521 tgacttcatg agcggtaagg ataacggcac cgtgttcttt ggcatccagc atgtttgctg
     8581 cggtcagacg aaatggatca acggtgccat ccggaacttt caccgcgcca atcagtgccg
     8641 ggttaacggc aggttcgata atgcgcgctt gctgcgggtc tatagcttct gcgctgatcc
     8701 ctgcttcttc gcaggcgcga ataaaagtgg cctggaatga gaggtcatct tccggcaggg
     8761 tgataaacag gccgttggtt ggttcaacgc agtgacgtgc aatgcgtttc aggatctggt
     8821 tttcactaat gcattcgcgg gccgattccg catcggttac cgcatagcgc gcaccgctgt
     8881 gcagcaggcc gtggttacgc ccggtggcac cggttgcgat gtcgtggcgc tcaaccaaaa
     8941 tcacgcgcag cccgcgcagg gcacagtcgc gggcaatccc ggctcccgtt gcgccgccgc
     9001 caatgataat cacgtcactt gattgcgagt cgcgagtttt cattgttatc cctctgaagt
     9061 tcgtttttta ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc
     9121 tcataattcg aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat
     9181 ttaataatgt gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa
     9241 ctcaagaaac ggcaggttct ctcactgaat caggctgtta atcataaata agaccacggg
     9301 ccacggaggc tatcaatgtt gagtattttt aaaccagcgc cacacaaagc gcgcttacct
     9361 gccgcggaga tcgatccgac ttatcgtcga ttgcgctggc aaattttcct ggggatattc
     9421 tttggctatg cggcttacta tttggttcgt aagaactttg cgcttgctat gccttatctg
     9481 gttgagcagg gattctcacg cggtgattta ggttttgccc tttcggggat ctcgattgct
     9541 tatggatttt cgaaattcat catgggttcg gtatcggatc gctcgaatcc gcgcgttttc
     9601 ctgcccgcag gtttgattct ggcggcggca gtgatgttgt ttatgggctt tgtgccatgg
     9661 gcgacgtcga gcattgcggt gatgtttgta ctgttgttcc tctgcggttg gttccagggg
     9721 atggggtggc cgccgtgtgg tcgtactatg gtgcactggt ggtcgcagaa agaacgtggc
     9781 ggcattgtgt cagtgtggaa ctgtgcgcac aacgtcggtg gtggtattcc gccgctgctg
     9841 ttcctgctgg ggatggcctg gttcaatgac tggcatgcgg cgctctatat gcccgctttc
     9901 tgcgccattc tggtggcatt attcgccttt gcgatgatgc gcgatacccc gcaatcttgt
     9961 ggtttgccgc cgatcgaaga gtacaaaaat gattacccgg acgactataa cgaaaaagcg
    10021 gaacaggaac tgacggcgaa gcagatcttc atgcagtacg tgttgccgaa caaattgctg
    10081 tggtatatcg ccatcgccaa cgtgttcgtt tatctgctgc gttacggcat cctcgactgg
    10141 tcaccgactt acctgaaaga ggttaaacat ttcgcgctgg ataaatcttc ctgggcctac
    10201 ttcttctatg agtatgcggg tattccgggc accctgctgt gcggctggat gtcggataaa
    10261 gtcttccgtg gcaaccgtgg ggcaaccggc gttttcttta tgacactggt gaccatcgcg
    10321 actatcgttt actggatgaa cccggcaggt aacccaaccg tcgatatgat ttgtatgatt
    10381 gttatcggct tcctgatcta cggtcctgtg atgctgatcg gtctgcatgc gctggaactg
    10441 gcaccgaaaa aagcggcagg tacggcagcg ggctttaccg ggctgtttgg ttacctgggc
    10501 ggttcggtgg cggcgagcgc gattgttggc tacaccgtgg acttcttcgg ctgggatggc
    10561 ggctttatgg taatgattgg cggcagcatt ctggcggtta tcttgttgat tgttgtgatg
    10621 attggcgaaa aacgtcgcca tgaacaatta ctgcaaaaac gcaacggagg ctaatggcat
    10681 gaaattgaag ctgaaaaacc ttagcatggc gatcatgatg agcactatag tcatgggaag
    10741 cagtgcaatg gcggcggaca gcaacgaaaa aatagtcatc gcccatcgcg gtgccagtgg
    10801 atatttgccg gagcatacgc tgccagcaaa agcgatggcg tatgcgcagg gagcggatta
    10861 tctggaacag gatttggtga tgaccaaaga cgaccatctg gttgttctgc atgaccatta
    10921 tctcgatcgt gttactgatg ttgccgatcg tttcccggat cgggcgcgca aagacggtcg
    10981 ttactacgcg atagatttca cgctggatga aattaagtcg ctgaaattta ccgaaggttt
    11041 cgatattgaa aacggtaaaa aagtacagac ttatccgggg cgtttcccaa tgggtaagtc
    11101 cgacttccgg gtgcacacct ttgaagaaga gattgaattt gttcaggggt taaatcactc
    11161 taccgggaaa aatatcggta tctatccaga aattaaagcg ccgtggttcc atcatcagga
    11221 agggaaggat attgcggcaa aaacgctgga agtgctgaag aaatatggtt acaccggtaa
    11281 ggacgacaaa gtttatttgc aatgttttga tgctgatgag ctgaagcgta ttaagaatga
    11341 gctggaaccc aaaatgggca tggatctcaa tctggtacag ctgattgcct ataccgactg
    11401 gaatgaaacg cagcagaaac agccggatgg aagctgggtt aattacaact acgactggat
    11461 gtttaagccg ggtgctatga aacaggtggc ggaatatgcc gacggtatcg ggccggatta
    11521 ccatatgttg attgaggaaa catcgcagcc gggtaatatc aaactcactg gcatggtgca
    11581 agatgctcag cagaataaac tggtagtgca tccttatacc gtgcggtcag ataaactgcc
    11641 tgaatacact actgatgtga atcagttata tgatgctctg tataacaaag cgggtgtaaa
    11701 tgggctgttt actgatttcc ctgataaggc agtaaaattc cttaataaag agtaatgcta
    11761 ctgcaggatg gcgttcatgc gccatcctgt ctcacatcaa atttttccat tcaccacgag
    11821 ttgttccgta cacgtttgta gtaaaaacag tggactgcct tctaccggct tttctttagc
    11881 caacaacgaa atctgtcgat agaaggttgg ttcgtgagga acggaacaca attcacctgc
    11941 aatgcttttc agcgttaact ctggttgtaa ggcgatgcct aatccctgtc gaataaactt
    12001 catcgctgta tccgactgat taaattcaca ttttatctac tgggcgcact attagtggta
    12061 gtggctggtg gaatctatgc gctgcaaatg aagggtaaaa gcggtgtcac gctagtagtg
    12121 gcaaaagaaa ttcattaata cgacacgttt cattaagatt ttcctcaggt aaccacctat
    12181 agtcattgtc gttaataaaa atgacaggcg atagggtatg gcagtttcag caaagtacga
    12241 cgaatttaat cactggtggg caacagaggg cgactgggtt gaagagccta actatcgccg
    12301 taatggtatg agcggggtgc aatgcgtcga gcgcaacggc aaaaagctgt atgtaaagcg
    12361 catgacgcat catctgtttc attccgtacg ttatccgttc ggccgaccaa cgattgtccg
    12421 tgaggttgcg gtaattaaag aactggaacg ggccggtgtc atcgtgccga agatcgtttt
    12481 tggtgaagcg gtgaaaattg agggtgaatg gcgagcgttg ctggtgactg aagatatggc
    12541 ggggttcatc agcattgctg actggtatgc ccagcatgca gtatcgcctt attctgacga
    12601 agtacggcaa gccatgttga aagcagtggc gctggcgttt aagaaaatgc atagcattaa
    12661 tcgtcagcat ggctgttgtt atgttcgcca tatctatgtg aaaacagaag gtaatgcaga
    12721 agctggtttt cttgacctgg agaaaagtcg tcgccgcttg cgtcgcgata aagcgattaa
    12781 tcatgacttc cgccagctgg aaaaatatct ggagccaatc cccaaagcgg actgggagca
    12841 ggtgaaagcg tattactacg ccatgtaatc gttgtaggcc agataagacg cataagcgtt
    12901 gtatctggca ctacaaccaa ttcacatctc gatttcaata tcgccttttg cccggcaaca
    12961 acagggcaaa atttcccccg gctgaataaa ggctaacggt tcggcaatcc agtcaacctg
    13021 acctgcaacc agacgggtgc gacaggagcc gcagtaacct tcgcgacact gatactcaac
    13081 cgccacattg tgggattcca gcgccgccag aagggaaggg tgttcatcct ggcacagcag
    13141 ttgtgtgcca gtgatgcgca gggtaacgcg ggccatcaga gttggaagtt actcaaatcg
    13201 tcggtgtcca cttccgcgtc aatctgcccg accagataag aactgacttc cacttcctgc
    13261 ggagcaacct gcacgttatc agacaccagc caggtgttga tccatgggat cgggttggag
    13321 cgcgtctgga acggcagatc caaaccgact gcctgcatac ggatattggt gatgtattca
    13381 acgtactggc agagaatgtc tttattcaga ccaatcatcg aaccgtcgcg gaacagataa
    13441 tccgcccagt ctttctcctg ttgagctgcc tgaacaaaca ggtcatagca ctcctgctta
    13501 cactcttcgg caatttccgc catctcagga tcgtccgcgc cgctgcgcag cagattcagc
    13561 atatgctggg tgccggtcag gtgcagggct tcgtcgcggg caatcaggcg aataattttg
    13621 gcgttgcctt ccatcaattc gcgttctgca aatgcgaagg aacaagcaaa gctgacgtag
    13681 aaacgaatcg cttccagcgc gttaacgctc atcaggcaga gatacagttt tttcttcagc
    13741 tcgcgcaggc taacggtcac agttttaccg ttaacggtgt gggtaccttc gcccagcaga
    13801 tgccagtagc tggtcatttc gatcagctca tcgtaatagc tggagatccc ttccgcacgt
    13861 ttctggatct gctcgttggt gacgatatcg tcaaacacaa cagacggatc gttaacgata
    13921 ttacgaatga tatgagtata ggaacgggaa tgaatcgttt ctgagaacgc ccaggtttcg
    13981 acccaggtct ccagttccgg gatcgagatc agcggcagca gtgccacgtt cgggctacgg
    14041 ccctgaatgg aatccagcag cgtctgatat ttcaggttgc tgataaagat gtgtttttcg
    14101 tgttcaggca gtgcctggta atcgatacgg tcgcgagaaa cgtcaacttc ttccggacgc
    14161 cagaagaaag agagctgctt ttcgatcagc ttttcgaaga tgtcatattt ttgctgatcg
    14221 tagcgagcca cgttgaccgg ctgaccaaag aacatcggtt ctttgagctg atcatttttc
    14281 gtctgtgaaa aggtggtata tgccatgagt gtgtcctgtt gggagtttaa tgccggataa
    14341 ggcattttac gccgcatcct gtgcctgatg cgacgctgac gcgtcttatc aggcctacaa
    14401 atccgagccg taggccggat aaggcgttca cgctgcatcc ggcatctcaa tatcagatct
    14461 tacatgcgcc gctttcgcag ccatcgtcct ggattgacgg taccagatcg tcctgcgcgt
    14521 cttcagcgcc gtcacgggtg ttctgataat acagcgtttt gaccccgaat ttgtaggcgg
    14581 tgagcaggtc tttcagcaac tgctgcatcg gcacttttcc tgacgggaaa cgtgacggat
    14641 cgtagttggt gttagcagag atcgactgat cgataaattt ctgcatgata cctaccagtt
    14701 gcaggtagcc atcgttaccc ggcatttccc acagcagctc ataggcgtcg tgcaggtgct
    14761 cgtagtccgg caccacctgg cgcaaaatac cgtctttcga cgctttgatg ctgacgtaac
    14821 cgcgcggcgg ttcaataccg ttagtggcgt tagagatctg tgaagaagtc tctgacggca
    14881 tcagggcaga aagcgtggag ttacgcaggc cgtgcgtttt gattgattca cgcaatgctt
    14941 cccagtcgta atgcagcggc tcattagcga tggtatccag atctttctta taggtatcga
    15001 tcggcaggat ccctttcgag taagtggttt cgttaaacca cgggcacgcg ccttgctctt
    15061 ttgccagctc gttagacgct ttcagcaggt aatactgaat cgcttcgaag gttttatgcg
    15121 tcaggttgtt ggcgctgccg tcggagtaac gcacaccgtg cttcgccagg tagtaagcga
    15181 agttgatcac accaataccc agcgtacgac gacccatcgc tccacgtttg gcggccggga
    15241 tcgggtaatc ctgataatcc agcagcgcgt caagtgcacg aaccgccaga attgccagct
    15301 cttccagttc atccaggcta ttaattgcgc ccaggttgaa agcagacagc gtacacagcg
    15361 cgatttcacc gttctcgtcg ttgacgtcgt tcagcggttt ggtcggcagg gctatctcca
    15421 ggcacaggtt agactgacgc actggcgcga tggccggatc aaacgggcta tgggtattgc
    15481 agtggtcaac gttctgaata tagatacgac cggtagacgc acgttcctgc atcatcagcg
    15541 agaacagctc aacggctttc acacgctgct tgcggatgct gtcgtctttc tcatatttgg
    15601 tatacaggcg ttcaaactct tcctgatcgg cgaagaatgc gtcgtacagc cccggtacgt
    15661 cggacgggct gaacagggtg atatcttcac ctttcagcag gcgggtatac atcagtttgt
    15721 tgatctgtac cccgtagtcc atatggcgca cgcggttgcc ttccacacca cggttgtttt
    15781 tcaacaccag caggctttcc acttccagat gccacatcgg gtagaacagc gttgccgcac
    15841 cgccgcgcac gccgccctga gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg
    15901 gaatacagcc ggtatggaac gcttcaccac cgcgaatcgg gctacccagc gcacgaatac
    15961 gcccggcgtt gatgccgatc ccggcacgct gggaaacgta tttcacaatc gcgctggagg
    16021 tagcgttgat ggaatccaga ctgtcaccgc actcgatcag tacgcaggag ctgaactgac
    16081 gagtcggggt acgcacgccg gacatgattg gcgtcggcag cgaaatttta aatgtggaaa
    16141 ccgcgtcgta aaaacgcttc acatattgca ggcgcgtttc acgcgggtag ttcgagaaca
    16201 ggcacgcggc aaccagaata taaaggaact gggcgctttc gtagatttcg ccggtcacgc
    16261 ggttctgtac cagatatttg ccttccagct gcttaacggc agcataagag aaggtcatat
    16321 cacggtcgtg atcgataaac gtgtccatct gcttgaactc ttcttccgtg tagtcttcca
    16381 gcagatgatt atcgtatttg cccatctcga ccattttcac cacgtggtcg tacagcgcag
    16441 gcggctcaaa ctggccgtag gcttttttac gcaggtggaa gatcgccagg cgcgcggcga
    16501 gatactgata atccggcgca tcacgggaga tcaggtctgc ggccgccttg ataatggttt
    16561 cgtggatgtc agaggtcttg ataccgtcat aaaactgaat gtgggagcgc agctcgacct
    16621 gggaaatcga aacgttatgc agtccttctg ccgcccaatc cagaacgcga tggattttgt
    16681 cgagattgat gcgctctgtg ctaccgtcgc gctttgtcac cagcagattc tgattcatgt
    16741 atgtcgtacc tgtttttgga attctttccg caatagtttc atgcagacta cctgtagtaa
    16801 gggaagctgc ttagaacact atatataggg ggtatgtttg ggattcactg caagatagtg
    16861 tgaaaatggc cctcttgcaa gtgcataact ttgtggataa ctcaggaagg aaaaagtggc
    16921 tttcgcgcac cttaggtcag acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg
    16981 ttaaaaataa ctgttcggcg atttcttgaa cggcagaata ggttggataa attgatatag
    17041 atggtttatt aatctttttg ctaatgattg caaaaattga tctaagcacg agtcatcaca
    17101 taatctgatg tatcagttgt ggtcaatata taaacgaatg taagcgattg ttgatgtcga
    17161 aatggttgtt ttctattcgg ttat
//
LOCUS       DAZFYC010000105        17161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFYC010000105 DAZFYC010000000
VERSION     DAZFYC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.105"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..347
                     /locus_tag="ACHKW5_003818"
     CDS             78..347
                     /locus_tag="ACHKW5_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7513116.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            316..1434
                     /locus_tag="ACHKW5_003819"
     CDS             316..1434
                     /locus_tag="ACHKW5_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO7513117.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KDKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(1511..1978)
                     /locus_tag="ACHKW5_003820"
     CDS             complement(1511..1978)
                     /locus_tag="ACHKW5_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFO7513118.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVELTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(1956..2159)
                     /locus_tag="ACHKW5_003821"
     CDS             complement(1956..2159)
                     /locus_tag="ACHKW5_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513119.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            2468..3262
                     /gene="potC"
                     /locus_tag="ACHKW5_003822"
     CDS             2468..3262
                     /gene="potC"
                     /locus_tag="ACHKW5_003822"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO7513120.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            3259..4305
                     /gene="potD"
                     /locus_tag="ACHKW5_003823"
     CDS             3259..4305
                     /gene="potD"
                     /locus_tag="ACHKW5_003823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO7513121.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            4363..4824
                     /locus_tag="ACHKW5_003824"
     CDS             4363..4824
                     /locus_tag="ACHKW5_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO7513122.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVLAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            4821..5609
                     /gene="ycfZ"
                     /locus_tag="ACHKW5_003825"
     CDS             4821..5609
                     /gene="ycfZ"
                     /locus_tag="ACHKW5_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO7513123.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5748..6569)
                     /gene="cobB"
                     /locus_tag="ACHKW5_003826"
     CDS             complement(5748..6569)
                     /gene="cobB"
                     /locus_tag="ACHKW5_003826"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO7513124.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(6585..7496)
                     /gene="nagK"
                     /locus_tag="ACHKW5_003827"
     CDS             complement(6585..7496)
                     /gene="nagK"
                     /locus_tag="ACHKW5_003827"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO7513125.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(7525..8769)
                     /gene="lolE"
                     /locus_tag="ACHKW5_003828"
     CDS             complement(7525..8769)
                     /gene="lolE"
                     /locus_tag="ACHKW5_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO7513126.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8769..9470)
                     /gene="lolD"
                     /locus_tag="ACHKW5_003829"
     CDS             complement(8769..9470)
                     /gene="lolD"
                     /locus_tag="ACHKW5_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO7513127.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(9463..10662)
                     /gene="lolC"
                     /locus_tag="ACHKW5_003830"
     CDS             complement(9463..10662)
                     /gene="lolC"
                     /locus_tag="ACHKW5_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO7513128.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10924..11997
                     /gene="ycfT"
                     /locus_tag="ACHKW5_003831"
     CDS             10924..11997
                     /gene="ycfT"
                     /locus_tag="ACHKW5_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO7513129.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLTASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFTPPSLPAAVSYSR"
     gene            12125..15571
                     /gene="mfd"
                     /locus_tag="ACHKW5_003832"
     CDS             12125..15571
                     /gene="mfd"
                     /locus_tag="ACHKW5_003832"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO7513130.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEATNLLPAHEFP
                     TDKAAIELFRSQWRDIFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15715..16677
                     /gene="ldtC"
                     /locus_tag="ACHKW5_003833"
     CDS             15715..16677
                     /gene="ldtC"
                     /locus_tag="ACHKW5_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO7513131.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16760..17017)
                     /gene="bhsA"
                     /locus_tag="ACHKW5_003834"
                     /gene_synonym="ycfR"
     CDS             complement(16760..17017)
                     /gene="bhsA"
                     /locus_tag="ACHKW5_003834"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO7513132.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4372 a         4390 c         4255 g         4144 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt tgatattctg caactggcgc
       61 gttccggagt gatagccatg agcgaacagt acctgataac gctcgacgag tggaaaccaa
      121 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa ctaggataca
      181 tcgttccaag accacaaaaa attcgtgggc gttggctgat agatcgccga gcagtatttg
      241 ttgggcctgg tgaaacggga attgcgccgg aaattcatac tggcgatgat gatgcactga
      301 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacgt agccggtctt
      361 tacgaaaagt ttgatcgcag aactggcaga gtttactacc agtataaaaa tcctgtgact
      421 ggaaaatttc acggactcgg aacagacaaa gataaggcag aaaaaatcgc ttccacagcc
      481 aatcagcgga tagctgcagc agaagctgaa tatttcatgc gcaaaattga tgaaagtccg
      541 tcagcaacaa aacgtcgggg tatcagatta aaggcatggg ttgatcgata tctgaaaata
      601 caggacacgc gactgaaaaa tggagatatt gcagctacaa ctcacaaaga aaaagcccga
      661 atggctgcat acctggtttc ccgtctggga aaccacccat tgaaagaact ggaagtaaga
      721 gactttgcat taatactgga tgagtggctg gataaagaca tggtcagcac agcgagagta
      781 aatcgtggat tatgggttga tatttataaa gaagcacagc atgcagggga agttcctcct
      841 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaactag agccaggctc
      901 accctggaag actggcaaaa aatttacaac gcaacgcctg aaaaacactt tatccgtaac
      961 gcaatgcttc ttgcgattgt tactggtcag cgccgtgatg acatttgcca catgcgtttt
     1021 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     1081 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagaagt tattgatggg
     1141 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccaaag
     1201 ccgatgagta aagacaacct gagcgactac tttgccaaag cacgggatct ggctgggata
     1261 actccaccag caggaaaaac tccgccaaca tttcatgaac aacgctccct gtcagaacga
     1321 ctgtaccgcg cgcagggtat tgatacaaaa acattactgg gacataaagt ccaggcaacc
     1381 accgatcgtt ataacgatac tcgtggtcag gaatgggtta aattggttgt ttgacgaaat
     1441 aaatgcagct gaaaaatgtt gatattgctt tgctagtgta aaaaccccgg catcaaccgg
     1501 ggttcggaga ctaatcttga tcaggttcct gcttagaata atgttttcca acaataaatg
     1561 ccgtaaccaa tgcaacaaga tctattgaaa cgagagttcc ggcaagaact gtctccccca
     1621 tgacgccaaa gacagtggca gctaagatga tcaatattgc caaccaaaaa gcctttgttt
     1681 gaccatctct ggcaatatca atgctatcag ccactgtttt atggcgatga gcctgctctt
     1741 tttcggtcaa ctcaacaagt cgatttgcaa gcccaggcac cagttgatca tactttttaa
     1801 gcattgaagg tggcggaaca ggcccctgaa aatgctggca aacaatagcc cgcacctgcg
     1861 gactatccaa aaccctgttt aagacctctg gattttcgat tactcgagaa accagttcat
     1921 tatccttttg ttcttcacaa gtgagtttgg tgttctcact ttctttttga tctggcattg
     1981 ttcagctttt caacgttaac ggataccatt gctttttgga aatcgcctct gatagccgca
     2041 gcatcctgac gcaaagcgga aactggattc gctggcatgc catttgctga aacgaccgtc
     2101 gggctcaggg ctataaccga gccaatggct tgtaacaaag ccacactttt acgcttcata
     2161 gtcactcctg agttcaatgg gattcaaaac ggattcaaag tgaatcttga tagctaattc
     2221 taataccaca ttatgtggtt tttcaacacg aagaaatcac atcaaaaatc tcattcggaa
     2281 agcgatatgg gtaaacaaac tggaatactt ttctgtccta gtaagctcac ccatgtataa
     2341 aaatgctgta aaatgcgtgg tcctgttttg gggaaaagtt ttggagagat tttggagaaa
     2401 ggaaaaaatt gaataaattc aaaatcctga aagtgtcccg ccttatgaat aaaaaggtgg
     2461 aactcgaatg atcggtcgac tgcttcgcgg cggttttatg accgctatct acgcgtacct
     2521 gtatatccca atcattattt tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa
     2581 ctggcagggt tttaccacca aatggtatag cctgctgatg aacaacgaca gcctgttaca
     2641 ggcagcgcag cattcactaa caatggcggt gttttcggcg acgtttgcta cgcttatcgg
     2701 ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg
     2761 aatgctgttt gtggtgatga tgtcgccaga tattgtgatg gcgatttctc tgctggtgct
     2821 gtttatgctg ctgggtattc agcttggctt ctggtcgctg ctgttctcgc atatcacctt
     2881 ctgcctgccg tttgtggtgg tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat
     2941 gctggaagcg gcgaaagatc tcggtgccag tgaatttacc attctgcgaa aaatcattct
     3001 gccactggca atgcctgcgg tggcggcggg ctgggtgtta agctttaccc tgtcgatgga
     3061 cgatgtagta gtttcttcgt tcgtcaccgg accaagctat gaaattctgc cgttaaaaat
     3121 ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac gcgctggcaa ccatattact
     3181 ggtgctgtcg ctggtgatgg taattgccag ccagcttatt gctcgtgata aaacgaaagg
     3241 taacacaggg gacgttaaat gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg
     3301 gcactgggca tgagcgccgc tcacgccgat gacaacaata cgctgtattt ctacaactgg
     3361 accgagtacg tgccgccagg actgcttgaa cagttcacca aagaaaccgg tattaaggtt
     3421 atctattcga cttacgagtc gaacgaaacc atgtacgcca agttgaaaac gtacaaagac
     3481 ggtgcctacg acctggtggt tccttcaacc tattacgtcg ataaaatgcg taaagaaggg
     3541 atgatccaga agatcgacaa gtcgaagtta agcaatttca gcaacctcga tccagacatg
     3601 ctcaacaagc cgtttgaccc gaataacgac tactccattc cgtatatctg gggggcgacg
     3661 gcgattggcg ttaacggtga tgcggtagat ccgaaatccg ttaccagctg ggccgatctg
     3721 tggaagcctg agtacaaagg tagcctgctg ctgaccgacg atgcccgtga agtgttccag
     3781 atggcgctgc gcaaactggg ctactccggt aacactaccg atccgaaaga gattgaagct
     3841 gcatataacg agctgaaaaa actgatgcca aacgtcgcag cgtttaactc cgataacccg
     3901 gctaacccgt acatggaagg cgaagttaac ctcggcatga tctggaacgg ctctgctttc
     3961 gttgcacgcc aggcgggtac gccaattgac gtggtgtggc cgaaagaagg cggcattttc
     4021 tggatggaca gcctggcgat cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg
     4081 atcaacttcc tgctgcgccc ggatgtggca aaacaggttg ccgaaactat cggttatcca
     4141 acgccaaacc ttgcggcgcg taagctgtta agcccggaag tggcgaacga taaaacgctc
     4201 tacccggatg ctgaaaccat taaaaatggt gaatggcaga atgacgttgg cgcagccagc
     4261 agcatttatg aagagtatta tcagaagctg aaagcaggac gttaattcag caaacgggcg
     4321 gtggtattac cgcccgtttt ttagttagca aggatgtggc taatgagtca ggacagtaaa
     4381 gttttatccc gcgcttttct cggtatcggt ctgatattgc tcgttatttc tgcaatcatc
     4441 ttttattacc attttacttt taccaaaagc gcggttcata cagaaggaat cattgttgat
     4501 gccgtttggt acaacaatca ctcaaacgac gtcgatgata atggttcctg gtatccagtg
     4561 cttgcctttc gccctacgcc agattacacg ctaattttca attcgaatat cggtagtgat
     4621 ttttatgaag atagcgaagg tgacagagtc aatgtctatt atccgccagg gcatccagaa
     4681 caagccgaaa taaacaatcc ctgggttaat ttttttaaat ggggctttgt tggcatagca
     4741 ggcattattt tcggtagtgt cggtctgata atcacgctcc cctccaccaa aaaatctcgc
     4801 aggaaacgga agtcccgccc atgaaaaaat tcattatact gctcagttta ctgatactcc
     4861 tgcctctggt agcaaccagt aaaccattga ttccaataat gaagacactt tttacggatg
     4921 ttaccggtac tgtaccggat gctgaagaga tcgcacgtaa agccgagctt tttcgccaac
     4981 aaacgggaat tgcaccgttt attgtgattt tacccgacat taataatgaa gccagtctca
     5041 gacaaaatgg taaagcgatg ctggcgcatg cgtcatcttc attgagtaat gtaaaaggaa
     5101 gtgttctgtt actatttact acccgcgaac cacggttaat tatgatcacc aacggccagg
     5161 ttgaaagtgg tctggacgat aaacatctcg gccttctgat agaaaatcac acgctggctt
     5221 atttaaatgc agatctctgg tatcagggaa tcaataatgc attggctgtt ctacaagcac
     5281 agatattaaa acaaccgacg ccgccactga cgtattatcc gcatccagga caacagcatg
     5341 aaaatgctcc ccccggcagc accaacacat taggttttat cgcctgggct gcgacgttta
     5401 tcttattttc ctggatcttt tgctacacga ctcgttttat ttatgcgtta aaatttgcag
     5461 tggcgatgac tattgccaat atgggttatc aggctttatg tttgtatatc gataatagct
     5521 ttgcgattac cagaatatcg ccactctggg cagggctaat tggtgtctgt acgtttattg
     5581 ccgcattgtt attgacgagt aaacgataaa agaggccgga aaagtgcgcg tgtattatgc
     5641 cggatggggc gcgaacgcct tatccggccc acaaaacccg caaattcaat taattgcgtc
     5701 cccttgtagg cctgataagc gtagcgcatc aggcaatgct tcctgcttta taatcccttc
     5761 agcaactttt caacaaactc aggcaccacc tggcttgccg ggccgtaata tttctcggca
     5821 aattcattac cgacctgact tggttcgaga ttcagttcca cggtgtgcgc gccatgcagt
     5881 ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc cggaagtacc aatggcaatg
     5941 aaaatatcag ccatcgacaa cgccatataa atttcatcca tgccgagtgg catttcgcca
     6001 aaccaaacga cgtgcgggcg caagggtgcc gggaactggc aacaatggca tttatcttct
     6061 ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg aactttcagc
     6121 aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag gttgtctata
     6181 ttctgcgtta ccagcaaaaa gcgatcgccg agggcgtctt gcagtttagc cagcgcaaga
     6241 tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc gttataaaac
     6301 gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc ttcaacccga
     6361 tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc cgcagaaatc
     6421 cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac cactttatct
     6481 ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt acgaaaacga
     6541 cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc ggttagatgt
     6601 aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc aatgcgcgga
     6661 acacgagcta caggtaagag atgacgaggc agcctgtccg ccagttgcgt tgtgattgcc
     6721 gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat ggtcaggata
     6781 tttcccagac aaaccgctaa taaatccaga taacgctcaa cgtgcgccct tgcctgctca
     6841 tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg ataatagtgt
     6901 tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc atgctgacca
     6961 cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc aaccggcaga
     7021 cgcatatggc caaactcgcc ggtaatgtag cttttaccgg taatcggttt gccgttgaaa
     7081 atcagcccgc cgccaacgcc ggtgccgaga atcaacccca tcaccagtgg atattgagta
     7141 aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc aaggcgtaca
     7201 tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt taccgctggc agcggggaca
     7261 ttggcggcat acagcgtacc atcttctgtt tccggcattc ccggaatacc gatgccgaca
     7321 gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac tgcatctaaa
     7381 aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa ctgccgaccg
     7441 ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta atacattgcc
     7501 gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag ggtcaatatt
     7561 actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg ctgtgaccag
     7621 tacgtagaag acgtccagcc agtgcaattc cgatggcagg aaatcaataa aatagatatc
     7681 gctggagagg aactgatgac cgatcaactt ttcaatccac tcaataatcg gggtaagttg
     7741 cagtgaaacc accacaccga taatcacccc acacaggctg ccgaacagcc ctgccagcaa
     7801 tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg ttcttaatac
     7861 tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga tgttgaaaca
     7921 ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca tctggatatc
     7981 gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc tgttggtcac
     8041 ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt taagggcaat
     8101 acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg ggatcatggc
     8161 aaaactgtga tcgagttgac cactcaactg caaaataccg gcaacgtgca aacgcacacg
     8221 ttttggctgc atcagtttat gctcgggatt cgagttgggg atcataatcg acacccaatc
     8281 cccctgcttc actttcagcg catccgccac gcctttgccg atgataattt gctgttcgcc
     8341 cgctttaaaa ttgcgccacg catcgccctg gacaaacgag ggtaatgcgc tcagacgctg
     8401 ttcctgttgc gggttaacgc ccttcacctg gattgcgcga agattcgcgc cactttccac
     8461 cagcccggtg aaattgatat acggcgcagc ggcggcaata cccggcactt tttgcacgtg
     8521 atccagtgct tcctgccagt tagtccacgg ctgatccacc gcctcgattt caccatgcgg
     8581 caccaccgcc agaatacggt tgttcagttc gcgttcaaag ccgttcatcg cgcttaagcc
     8641 gacgatcaat accgccacgc cgagggcaat gccaatggta gaaatgacag agattagcga
     8701 caccatgccg ccgcgtcgcc gtccgcgact aaaacgcagg ccaattaata acgataaagg
     8761 catcgccatt actccgcccc catcaggctc agttccgccg tcagacgacc atcacgcatt
     8821 tctaactggc ggctcatacg tttcgccagt tgcaggtcgt gagtaaccac caggaaggcg
     8881 gtgccctgca agcgattcaa ttccccaagc aactggaaga tgctgtcggc gttacgcgca
     8941 tcgaggttac cggtaggttc atccgccagt accaggcgcg ggttattcac cagcgcacgg
     9001 gcaatcgcca cacgctggcg ttcgccgcca gaaagttcag atgggcggtg attcgcacga
     9061 tgctccagcc ccaccgcttt taacatctca agtgcacggc tgttgatttc agcgggcttt
     9121 ttcttgccaa taagcagcgg catagccacg ttttccaggg cggtaaaatc cggcagcagg
     9181 tggtgaaact gataaataaa gcccagcttc tggttgcgca gctctgcttt cgccgccgaa
     9241 gacagtttgc tcatcggttg accgttaaag atcacatcgc cggaggttgg cgtatccagc
     9301 ccgcccaaca agtgcagcaa ggtgctttta ccggaaccag agctaccgac gatcgccatc
     9361 atttcacctt cgccgacgct gaaactgaca ttgtgcagca catcggtttg cacactgcct
     9421 tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat aacgtaaagc
     9481 ctcagcgggt tgagtggcgg cagcgcgcca tgaagggtaa agcgtagaca gcagcgcgat
     9541 agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcga ccggcagcgc
     9601 cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc tggcaagcag
     9661 ggcgccaagc gccgctccga ggatcgcacc gataatcccg gcgctggccc cttgcaccat
     9721 aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg ctacttcgcc
     9781 ctgcttctcc attaccatca gccctagtga ggtaataatg ttaaacgccg caacggcgac
     9841 aatcaggctc agcagtaaac ccatcatatt tttttccatg cgtacggcct ggaacaactc
     9901 gcctttacga tcacgccagt cctgccattt gctgccttca ggcagttttt gctgacttaa
     9961 tgagtcgact ttcagcggct catccagcca caaacgccag ccggtaatat tgcctgccgg
    10021 ataacgcatc agacgcgagg catcctcaat attcaccagc atttcattgc catcgacttc
    10081 actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa tacgccccat
    10141 cggcgtgaac tggctggcag atggtaccat catgcggatt tgatcaccgc gattaacgcc
    10201 tagctgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct cgaggtcagt
    10261 ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcgccgggt cgataccgag
    10321 catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg
    10381 tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct gcgggttaag
    10441 agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt tgttttgcag
    10501 ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca ccccgagggt
    10561 aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg cacgcccacg
    10621 catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg tctggttgct
    10681 gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga actgtaagtg
    10741 atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc catgttttat
    10801 catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca gatacccatt
    10861 acacctcggg tcataacccg caggatcaac cgcatgtaaa ttaaaaaaga gcgtgttgtc
    10921 ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat ttgtctggtg
    10981 gtgatttatc actcggtcat taccttttat ccgcatctga ccacttttca gcatccgtta
    11041 tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc ctttcgtatg
    11101 ccggtttttt tctttatttc cggctatttg attcgccgct atatcgacag cgtaccgtgg
    11161 ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct ttggggcgtg
    11221 gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg cgatttaagc
    11281 aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg gatgatcacc
    11341 gccagcacca gcttgtggta tctgtatgcg ttaattgtct atttcgtggt atgtaaaatt
    11401 tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgttgagtgt ggcggttaat
    11461 ttcgttccca cgccgtggtg gggaatgaac agtgtgatcc gcaatttgcc ttattacagc
    11521 cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc gttgcgccgc
    11581 catctgctga cggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt gtttactatc
    11641 tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta tcaatacgag
    11701 caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa caccattgct
    11761 atctacacca cccatcgcat tctggttgaa atattcagct taactctgct tgcgcaaatg
    11821 aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta tccctttgtc
    11881 agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca gcgtgcattc
    11941 agcgatctgt tgttcacccc gccttcactg cccgctgccg tcagttactc ccgctaagcc
    12001 atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat aatgaacagc
    12061 attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga atctgacaac
    12121 cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct
    12181 gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg aacgtcacgc
    12241 cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc atgatgaaat
    12301 cagtcagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc ttccctacga
    12361 cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt accagctacc
    12421 gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc
    12481 acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga
    12541 tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg tgatggagca
    12601 cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc
    12661 ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg acgtcgacag
    12721 ccagcgcacg ctggaggaag tagaagcgac caatctgctg cccgcgcacg aatttccgac
    12781 cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gatatcttcg aagtgaagcg
    12841 cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg ggatcgagta
    12901 ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt tccctgccaa
    12961 taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc aggctgacac
    13021 gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc caccacaatc
    13081 gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc gggtgcagct
    13141 aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc agaaactgcc
    13201 agacctggcc gttcaggcac aacaaaaagc gccgctggat gcgctgcgta agttcctcga
    13261 gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg
    13321 tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg atgaagccag
    13381 cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg ataaggtgcg
    13441 taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc gccgtcgtca
    13501 ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg agctgcatat
    13561 tggtcagccg gtggtccatc tggagcacgg tgtcgggcgc tatgccggaa tgaccacgct
    13621 cgaagcgggc ggcattactg gcgagtattt gatgctcacc tatgccaacg acgccaaact
    13681 gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg cggaagaaaa
    13741 cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga
    13801 aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg ccgccaaaga
    13861 gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt
    13921 tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca tgtgtcagcc
    13981 gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat
    14041 gcgcgccgct ttcctggcag tagataacca caagcaggtg gcggtgttgg tgcctaccac
    14101 ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact ggccggtacg
    14161 tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt
    14221 ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa
    14281 gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa
    14341 agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg caacgccgat
    14401 cccacgcacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc
    14461 gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc tggtggtccg
    14521 ggaggcgatc ctgcgtgaaa ttttgcgcgg ggggcaggtt tattatctct acaatgatgt
    14581 ggaaaacatc cagaaagctg ccgaacggct ggcagaactg gtgcctgaag cacggattgc
    14641 catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg atttccatca
    14701 tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg acatcccgac
    14761 agccaacacc attatcattg aacgtgcgga tcacttcggt ctggcgcagc tgcaccagtt
    14821 acgcggtcgc gtcggacgtt cgcatcatca ggcatatgca tggttgctga caccgcatcc
    14881 aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc tggaagatct
    14941 cggggcaggt tttgcgctgg caacgcacga tctggagatt cgcggcgcgg gtgaactgct
    15001 tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata tggagttgct
    15061 ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag atctcaccag
    15121 ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt tcatccctga
    15181 cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc gccaaaacgg aaaacgaact
    15241 ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac
    15301 cctgctggat gttgcccgtc tgcgccagca agcgcagaaa ctggggatca ggaagctgga
    15361 aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta atccggcctg
    15421 gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc cgacgcgcct
    15481 gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac gccagtttat
    15541 gcgtgaactg gaagagaacg cgatcgctta atggtaaatc gggggcgttt ctgcgccccc
    15601 atctgcaaca tttacaaatt ctttgcactt ccctgcacta tccgacactg tcaccatcca
    15661 taattcaggc ttatctgttt attacaataa ccttatattt attatggatt tttggtgatg
    15721 atcaaaacgc gtttttctcg ctggctaacg ttttttacgt tcgccgctgc cgtggcgctg
    15781 gcgctaccgg caaaagccaa cacctggccg ctgccgccag cgggcagtcg tctggttggc
    15841 gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc tggaagccat cgccaaaaaa
    15901 tataacgtcg gctttctcgc tctgttacag gctaaccctg gcgttgatcc ttacgtaccg
    15961 cgcgcgggta gcgtgttaac gatcccgttg caaaccctac ttccagatgc gccgcgcgaa
    16021 ggcattgtga tcaatattgc ggagctgcgt ctctattact acccgccggg taaaaattcg
    16081 gtaaccgtgt atccaatagg tattggtcag ttaggtggtg acacgctgac accgacaatg
    16141 gtgaccaccg tttcagacaa acgtgcaaac ccaacctgga cgccaacggc aaacatccgc
    16201 gcccgttata aagcacaggg aattgagttg cctgcggtag tgccggctgg accggataac
    16261 ccaatgggcc atcatgcgat tcgtctggcg gcctatggcg gcgtttattt gcttcacggt
    16321 acgaacgccg atttcggcat tggcatgcgg gtaagttctg gctgtattcg tctgcgggat
    16381 gacgatatca aaacactctt tagccaggtc accccaggca ccaaagtgaa tatcatcaac
    16441 actccgataa aagtctctgc cgaaccaaac ggtgcgcgtc tggttgaagt acatcagccg
    16501 ctgtcagaga agattgatga cgatccgcag ctgctgccaa ttacgctgaa tagcgcaatg
    16561 caatcattta aagatgcagc acaaactgac gctgaagtga tgcaacatgt gatggatgtc
    16621 cgttccggga tgccggtgga tgtccgccgt catcaagtga gcccacaaac tctgtaaatc
    16681 ctggataaaa aaaatccccg cgacacggca tgtcgcgggg aaaaaatcag cagtagcagg
    16741 cattaatgag ggttaatgct tatttataaa ttactgctgt tccatggagg gtattcggac
    16801 cggttacaga agtaatacgg aaagattttg cgcccatctc atccgctttt tgcgccagct
    16861 gctcttccag cgatcccaga tttgtccccg cgttagcact gattgtaccg actttttgtt
    16921 ggccttctgg cgttgactga acttcgacag ccgcaaagct ggcaaatgac atggagctta
    16981 aaatcgccgc agcgatgagg gtttttacgt ttttcataat agtggcctta tgcagatgaa
    17041 tgacgtcgca agcaattact taacgatcga taactaaatg atagatgtga tctggatcac
    17101 atacaagata ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagttaa
    17161 a
//
LOCUS       DAZFYC010000106        17038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFYC010000106 DAZFYC010000000
VERSION     DAZFYC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.106"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..617
                     /gene="yfiR"
                     /locus_tag="ACHKW5_003835"
     CDS             99..617
                     /gene="yfiR"
                     /locus_tag="ACHKW5_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO7513133.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            607..1833
                     /gene="dgcN"
                     /locus_tag="ACHKW5_003836"
     CDS             607..1833
                     /gene="dgcN"
                     /locus_tag="ACHKW5_003836"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO7513134.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1849..2331
                     /gene="yfiB"
                     /locus_tag="ACHKW5_003837"
     CDS             1849..2331
                     /gene="yfiB"
                     /locus_tag="ACHKW5_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7513135.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2408..2755)
                     /gene="rplS"
                     /locus_tag="ACHKW5_003838"
     CDS             complement(2408..2755)
                     /gene="rplS"
                     /locus_tag="ACHKW5_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO7513136.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2797..3564)
                     /gene="trmD"
                     /locus_tag="ACHKW5_003839"
     CDS             complement(2797..3564)
                     /gene="trmD"
                     /locus_tag="ACHKW5_003839"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO7513137.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3595..4143)
                     /gene="rimM"
                     /locus_tag="ACHKW5_003840"
     CDS             complement(3595..4143)
                     /gene="rimM"
                     /locus_tag="ACHKW5_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO7513138.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4162..4410)
                     /gene="rpsP"
                     /locus_tag="ACHKW5_003841"
     CDS             complement(4162..4410)
                     /gene="rpsP"
                     /locus_tag="ACHKW5_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO7513139.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4547..5908)
                     /gene="ffh"
                     /locus_tag="ACHKW5_003842"
     CDS             complement(4547..5908)
                     /gene="ffh"
                     /locus_tag="ACHKW5_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO7513140.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6075..6866
                     /gene="ypjD"
                     /locus_tag="ACHKW5_003843"
     CDS             6075..6866
                     /gene="ypjD"
                     /locus_tag="ACHKW5_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO7513141.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6887..8173
                     /gene="yfjD"
                     /locus_tag="ACHKW5_003844"
     CDS             6887..8173
                     /gene="yfjD"
                     /locus_tag="ACHKW5_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO7513142.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            8296..8901
                     /locus_tag="ACHKW5_003845"
     CDS             8296..8901
                     /locus_tag="ACHKW5_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO7513143.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTLWLEVQDFLS"
     gene            complement(8936..9526)
                     /gene="grpE"
                     /locus_tag="ACHKW5_003846"
     CDS             complement(8936..9526)
                     /gene="grpE"
                     /locus_tag="ACHKW5_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO7513144.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            9649..10527
                     /gene="nadK"
                     /locus_tag="ACHKW5_003847"
     CDS             9649..10527
                     /gene="nadK"
                     /locus_tag="ACHKW5_003847"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO7513145.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10613..12274
                     /gene="recN"
                     /locus_tag="ACHKW5_003848"
     CDS             10613..12274
                     /gene="recN"
                     /locus_tag="ACHKW5_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO7513146.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12423..12764
                     /gene="bamE"
                     /locus_tag="ACHKW5_003849"
     CDS             12423..12764
                     /gene="bamE"
                     /locus_tag="ACHKW5_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO7513147.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12826..13116)
                     /gene="yfjF"
                     /locus_tag="ACHKW5_003850"
     CDS             complement(12826..13116)
                     /gene="yfjF"
                     /locus_tag="ACHKW5_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO7513148.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(13106..13543)
                     /gene="ratA"
                     /locus_tag="ACHKW5_003851"
     CDS             complement(13106..13543)
                     /gene="ratA"
                     /locus_tag="ACHKW5_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO7513149.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13714..14196
                     /gene="smpB"
                     /locus_tag="ACHKW5_003852"
     CDS             13714..14196
                     /gene="smpB"
                     /locus_tag="ACHKW5_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO7513150.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14411..14773
                     /gene="ssrA"
                     /locus_tag="ACHKW5_003853"
     tmRNA           14411..14773
                     /gene="ssrA"
                     /locus_tag="ACHKW5_003853"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            15045..15293
                     /locus_tag="ACHKW5_003854"
     CDS             15045..15293
                     /locus_tag="ACHKW5_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7513151.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(15304..15375)
                     /locus_tag="ACHKW5_003855"
                     /pseudo
     CDS             complement(15304..15375)
                     /locus_tag="ACHKW5_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            15460..15543
                     /locus_tag="ACHKW5_003856"
                     /pseudo
     CDS             15460..15543
                     /locus_tag="ACHKW5_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(15664..16677)
                     /gene="nleC"
                     /locus_tag="ACHKW5_003857"
     CDS             complement(15664..16677)
                     /gene="nleC"
                     /locus_tag="ACHKW5_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO7513152.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
BASE COUNT         4402 a         4399 c         3925 g         4312 t
ORIGIN      
        1 ttctcacatt gatgacgatc gcatgaaaaa atgatatttt aatgagcatt ctgaaaaaat
       61 atcgatgcat ttcgagcgaa gatggtgggg atccctgaat gcgtttttct caccgactgt
      121 tcttacttct gattctcctg ctgacgggag caccaatact cgctcaggag ccttctgacg
      181 ttgcgaaaaa cgtgcgtatg atggtttccg gtattgtcag ttacacccgc tggcctgcgc
      241 tatcagggcc accaaagtta tgtatctttt catcttcacg ttttagtacc gccctacaag
      301 agaatgccgc gacatctttg ccttatctgc ctgtcatcat tcacactcaa caagaggcga
      361 tgatttcagg ctgcaatggt ttttattttg gcaacgagtc accgacattc cagatggaat
      421 taaccgaaca atatccgtca aaggcgttat tattaattgc cgaacaaaat accgagtgca
      481 ttattggtag cgccttttgt ctgatcatcc acaataatga cgtcagattt gccgtaaacc
      541 tggatgcctt atcgcgtagc ggtgtaaagg tcaacccgga tgtcttaatg ctcgcacgga
      601 agaaaaatga tggataacga taattctctt aataagcgcc ccacgtttaa aagagcatta
      661 cgcaacatca gtatgaccag catatttatc actatgatgc tgatctggtt gctgctttcc
      721 gtgacctcgg tgctgaccct gaaacagtac gcgcaaaaaa acctggcact gacagcagca
      781 acaatgactt acagtctgga agcagctgtc gtttttgccg atggccctgc agcaactgaa
      841 acactggcag cgctgggcca gcaagggcaa ttttcaactg cagaagtacg tgataagcag
      901 caaaatattc tggcgtcctg gcattacacc cgtaaggatc caggcgatac tttcagcaat
      961 ttcataagcc actggctctt ccccgccccc atcattcagc cgattcgtca caatggtgaa
     1021 accattggcg aagtacgctt aaccgctcgc gacagttcaa tcagccattt tatctggttt
     1081 tcgctcgccg tactgaccgg ttgtattctg ctggcatcag gcatcgcaat taccctcacc
     1141 cgccatttgc acaatggcct ggtagaagcg ctgaaaaata tcaccgatgt cgtacatgat
     1201 gtgcgttcca accgcaattt ttcccgacga gtttcggaag aacgtatcgc tgagtttcac
     1261 cgcttcgctc tcgacttcaa cagtctgctg gatgaaatgg aagagtggca gcttcgttta
     1321 caggccaaaa atgcgcagct tctgcgtacc gcgctacatg acccattaac cgggctggct
     1381 aaccgcgcag cgtttcgtag cggcatcaat acgttgatga acaattccga tgcccgaaaa
     1441 acgtcggcgt tactatttct tgatggcgat aatttcaaat atatcaatga tacctggggt
     1501 catgcgacgg gcgatagagt cttgattgaa atcgcaaaac ggttagctga atttggcggg
     1561 ctgcgacata aagcataccg cctgggcggc gatgaattcg ctatggtgct ctatgatgta
     1621 cagtcggaat ctgaagtgca gcagatatgc tcagcactga cacaaatctt taatctccct
     1681 tttgatcttc ataatggcca tcagaccacc atgacattaa gcattggtta cgcgatgacc
     1741 attgagcacg cctctgcgga aaaattacaa gagcttgccg atcacaatat gtatcaggcc
     1801 aaacaccagc gtgccgaaaa gctggtgaga taacaaggat atatcgatat gataaagcac
     1861 ctggtagcac ccctgatttt cacctcacta atactgactg gctgccagtc ccctcaggga
     1921 aagtttactc ctgagcaagt cgccgctatg caatcttatg gatttactga atccgccggc
     1981 gactggtcgc tgggcttatc agatgccatt ctgttcgcaa aaaatgacta caaattgctc
     2041 ccggaaagcc agcaacagat ccaaaccatg gcagccaaat tggcctcgac agggctaaca
     2101 catgcccgta tggatggaca caccgataac tatggtgaag acagttacaa cgaaggctta
     2161 tcattgaaac gggcgaatgt cgtggccgat gcatgggcta tgggtggaca aattccacgc
     2221 agcaatctca ccacacaggg tttaggaaaa aaatatccca tagccagtaa caagaccgcc
     2281 cagggccgcg ccgagaaccg ccgcgtcgca gtggtgatta caacccctta aaacacgagc
     2341 aaacagacaa aaaaagggcc agccaattgg ccagcccttc ttaacaggat gtcgcttaag
     2401 cgaaatctta gttaagacgc tctttgatac gagcagcctt accagtacgc tcacgcaggt
     2461 agtacagttt agctttacga acagcaccac gacgtttgac agaaatgctg tcaactaccg
     2521 gagagtgagt ctggaagaca cgctcaacgc cttcgccgtt ggaaatttta cgaacagtga
     2581 atgcagagtg cagaccgcgg ttacgaatag cgataaccac gccctcgaat gcctgcagac
     2641 gttttttgga accttcaaca acccatactt tcacttccac ggtatcaccc ggacggaagg
     2701 aaggtacgtc ctgcttcatc tgctcttgtt caagttgctt aataatgttg ctcataattt
     2761 aatctcttat cctgggtaaa ctgatatctc gggggcttac gccatcccat catgtttatg
     2821 ttgctgttgt gcgtgttccg ttttgaactc cgccagcaac cttgcttgct cttcagtcag
     2881 agccaggttt tccagaagtt caggtcttct aagccaggta cggcccagcg actgtttcaa
     2941 acgccagcga cgtatctcgg catggttgcc cgacagtaac actggcggaa cttccatccc
     3001 ttctaacacc tcaggccgcg tatagtgcgg gcaatccagc aatccttcag caaaggaatc
     3061 ttccgttgcc gaggcttcat gtcccagtac tcccggaata aaccgggaaa cggagtcaat
     3121 cagcgtcatt gctggtaact caccaccact gagaacgtaa tcgccgattg accattcttc
     3181 gtcaatttcg gtttggatca cgcgctcatc tataccttcg tagcgaccgc acaccagaat
     3241 caatttttga ttcgttgcca gttcgctgac gcccgcttga tcaagcttgc gtccctgtgg
     3301 tgacagataa atcacctttg cgccttcacc cgccgcggct tttgctgcat gaatggcgtc
     3361 ccgcaagggt tgcaccatca ttaacatccc cggtccgccg ccgtaaggac gatcgtccac
     3421 ggtacggtgc cggtcatgcg tgaagtcgcg aggactccaa ctctggatgc tcagcaggcc
     3481 atttttaact gcccggccag ttaccccgta atcggtaatt gcgcggaaca tttcaggaaa
     3541 caggctaatt atgccaatcc acatagcgcc gtcttttacc gtttatccgg tggtttaaaa
     3601 accaggatcc caatctactt cgattgaacg agtagtgaga tcgactttct tgataacctg
     3661 cccatcgagg aacggtacga gacgttcctt gataccaaac gcatctttca ggtttgcctt
     3721 aatgacgaga acgtcattag atccggtttc catcatatcg acgactttac cgagatcgta
     3781 gccttcagtg gttactacct ggcagcccat caggtctttc cagtagtagt caccctcttc
     3841 aagctgaggc agctgcgatg aatccacgac aatttcacaa ttcgtcagca ggttcgccgc
     3901 atcacgatcg tcaacgcctt tcagcttgat gatcatgtcc tgattgtggt gcttccagct
     3961 ttccagctgg acttgctgcc actgacccgc cttctggata aaccagggct gatagtcaaa
     4021 aatgctttcg gcgtcttcgg tggaagaaaa cactctgagc cacccacgaa taccgtaaga
     4081 cgaacccatt tttcccaaaa cgatgggatc aacaggtgct tgcgcggtga gttgtttgct
     4141 catcatgacc accgtgacag attaagctgc tttgtttact tctttgatca gcgcagcaac
     4201 gcgatcagaa atagttgcgc cctggccaac ccagtgagcg atgcgatcca gatccaggcg
     4261 agtgccttct tctttttcgc tagcgattgg gttgaagaaa ccaacgcgct cgatgaagcg
     4321 accgttgcgt gcattacggc tgtcagcgac aacaacctgg tagaacggac gctttttagc
     4381 gccgtgacgt gctaaacgaa tagttaccat aacatcctct tgtgtgaata aaacaaccgg
     4441 accccatcga ggaacggagt ccggtgtcat attaaaagcc cgaaaatttt actgatttct
     4501 ggagaaaatg caatcagcag ttaataactc tgtcgcataa ggtcatttag cgaccaggaa
     4561 atcccggcgg catcatcccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
     4621 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggttc acgtcctgca
     4681 cctgcatacc gcaaccggca gcgatacgac gcttacgcga acctttgatg atttctggct
     4741 tagcgcgctc tttcatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
     4801 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
     4861 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
     4921 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
     4981 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
     5041 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
     5101 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
     5161 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
     5221 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
     5281 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
     5341 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
     5401 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
     5461 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
     5521 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
     5581 cttgcaggcc cgccatcagt acgaccgcag gcggttgcgc agccaggttc agggtctggt
     5641 tctcttcgcc catcgccgca accagttcgt tacggactat tttgacgaac tcctgccccg
     5701 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
     5761 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
     5821 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
     5881 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
     5941 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
     6001 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
     6061 ctgtcgacat tactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
     6121 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
     6181 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
     6241 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
     6301 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
     6361 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
     6421 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
     6481 ccacgctaat tatcgccgcc ctgtacgcgc tgcaactggc gtggattgat taccaactga
     6541 agaacaagaa gctggcattt aaccaggaaa tgccgccatt gatgagtatc gagcgtaaaa
     6601 tgttccacat cacgcagatt ggcgtggtgc tgctaacgct cacgctttgc actggcctgt
     6661 tctacatgca caacctattt agcatggaaa atatcgacaa ggctgtgctc tctatcgtgg
     6721 cgtggtttgt ctatattgtg ctgctgtggg gacattacca tgaaggatgg cgtggacgcc
     6781 gcgtcgtctg gtttaacgtt gcgggcgcgg taattctgac actggcctac ttcggcagcc
     6841 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
     6901 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
     6961 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
     7021 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
     7081 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
     7141 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
     7201 actggtattt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
     7261 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
     7321 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
     7381 tagcggctct ttgagcaaag aagagttgcg cactatcgtg cacgaatcac gctcacaaat
     7441 ttcccgtcgc aatcaggata tgctgctgtc ggtgctcgat ttggaaaaaa tgaccgttga
     7501 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
     7561 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
     7621 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
     7681 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgccggaagg
     7741 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
     7801 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
     7861 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
     7921 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
     7981 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
     8041 agagatccct gtcgcaggca cccgcgtgcg tattggcgag tacgatatag atattctcga
     8101 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
     8161 tgtggcggag taacgcaatg gcgaactccg gttcgccttt tttttatcgc cgtcttttag
     8221 gattccatca attcctgtca tttacttagc aatgtaataa tgtcttttca agcattcgga
     8281 aaggatattg atgttatgtc agaagattat gtaattgaat gggataagga ttttgcagac
     8341 gatctcaacg tcgtcgccag cgttttttta cagcacaccc caaccttatg gcccactatt
     8401 ttctctcaac tttcgacgca acctgaaatc tttgaagacg aagatgaaga cgaatatgga
     8461 ttgcaggacg ttctcgattg cagtggtggc gatctcggga atcgtgacct ggcgcaggca
     8521 tttttacagg ttctacgcgg tgaaggatta attcaactcg ttgactggaa aggtgaagat
     8581 gaagaaggtg agctggctaa ttttgctgca gatcgctttt atgagctcac aaaaaacctg
     8641 atagcttctg aggaattaag gagtcttctt gtcgaaataa cccaagagga tgaaatctcc
     8701 gatgtctgtg aagccggaga ccgctacctt gacgaaatct ttgaacgcat ccaaaccgaa
     8761 ctcaataaaa ggggctttca gatttttgat ctgaatgaag gatctgacac ctataacgtg
     8821 gtcgttctgc caatgaacga atataaaaaa atagatgatt tcaacacgct gtggctggaa
     8881 gtacaggatt ttttaagcta aaaaaatggc cgacttcgcg tcggccatca gatatttact
     8941 tcgccttcgc tacagtaacc atcgccgcac gaatcgtacg accattcagc gtataaccct
     9001 tctgcataat gcccagtacg ttacctggcg caacgtcatc agattccacc attgcgatag
     9061 cctgatgcac attcgggtcc agcggcacgt tagtttcggc gatcacttca acaccaaact
     9121 tacgcacaac atccagcatc gacttcagcg tcagctcaat gccttcaacc atcgcagaca
     9181 tatccgggtt agctttatca gccacttcca gcgcacgatc caggctatca atcaccggca
     9241 gcaattcgtt gatgaatttc tccagcgcga atttgtgggc tttttcaata tccagttcag
     9301 tacgtcgacg caggttttcc atttcggctt ttacacgcaa aatgccgtca cgttcacggg
     9361 tctgggcttc agccagctga gcttcgagat tcgcaatttt ttcatcgcgc ggatccacct
     9421 gctcagcaga agcttctggc tcaactgcct caatctcttc gtgctgatcc atgataattt
     9481 cttccggggc ttgcccctca ggcgttttct gttctttact actcatgaat ttctccgcgt
     9541 ttttttcgca ttcatctcgc taacttcgct tattatgggg atcagtttca gggtttcaag
     9601 ggaagcactc acattgtcat caatcttcgc aacaaggacc tcggaaaaat gaataatcat
     9661 ttcaagtgta ttggcattgt gggacaccca cggcacccca ctgcactgac aacacatgaa
     9721 atgctctacc gctggctgtg cacaaaaggt tacgaggtca tcgttgagca acaaatcgct
     9781 cacgaactgc aactgaagaa tgtgaaaact ggcacgctcg cggagattgg gcaactagct
     9841 gatctcgcgg tagtcgttgg tggcgacggt aatatgctgg gcgcggcacg cacactcgcc
     9901 cgttacgata ttaaagttat tggaatcaac cgtggcaacc tgggtttcct gactgacctt
     9961 gaccccgata acgcccagca acagttagcc gatgtgctgg aaggccacta catcagcgag
    10021 aaacgttttt tgctggaagc gcaagtctgt cagcaagatt gtcagaaacg catcagcacc
    10081 gcgataaatg aagtggtgct tcatccaggc aaagtggcgc atatgattga gttcgaagtg
    10141 tatatcgacg agatctttgc gttttctcag cgatctgatg gactaattat ttcgacgcca
    10201 acaggctcca ccgcctattc cctctctgca ggcggtccta ttctgacccc ctctctggat
    10261 gcgattaccc tggtgcccat gttcccgcat acgttgtcag cacgaccact ggtcataaac
    10321 agcagcagca cgatccgtct gcgtttttcg catcgccgta acgacctaga aatcagttgc
    10381 gacagccaga tagcactgcc gattcaggaa ggtgaagatg tcctgattcg tcgctgtgat
    10441 taccatctga atctgattca tccgaaagat tacagttatt tcaacacatt aagcaccaag
    10501 ctcggctggt caaaaaaatt attctaattt tacgccagcc tctttactgt atataaaacc
    10561 agtttatact gtacacaata acagtaatgg tttttcatac aggaaaacga ctatgttggc
    10621 acaactgacc atcagcaact ttgctatcgt tcgtgagctt gagattgatt ttcatagcgg
    10681 catgaccgta ataactggcg agaccggcgc gggtaaatct attgcaatag atgccctcgg
    10741 tctttgtctc ggtggtcgcg ctgaagccga catggtgcgt accggcgctg ctcgcgctga
    10801 cctgtgcgcc cgtttttctc tgaaagatac gccagcggct ctgcgctggc tggaagaaaa
    10861 ccagcttgaa gacgggcatg aatgtttgct tcgtcgcgtg atcagcagcg atggtcgctc
    10921 ccgtggtttc atcaacggta cagctgttcc tctgtcacaa ctgcgcgaac tgggtcagtt
    10981 gctgattcag atccatggtc agcacgctca tcagttactc accaaacccg agcaccaaaa
    11041 gttcctgctt gatggctatg ccaatgaaac ctctctactc caggaaatga cagcacgtta
    11101 tcagttgtgg catcaaagct gccgtgacct cgcgcatcat caacagttaa gtcaggaacg
    11161 cgctgcccgt gcggaactgc tgcaatacca attaaaagaa cttaacgaat ttaatccgca
    11221 gcccggagag tttgaacaaa tcgacgaaga gtacaaacgt ctggcgaaca gcggtcaatt
    11281 gctgaccacc agccagaatg cattggcatt aatggccgac ggtgaagacg caaacctgca
    11341 aagtcagctt tacacggcta aacaactggt gagcgaattg attggcatgg acagcaaact
    11401 gtccggcgta cttgatatgc tggaagaagc taccatccag attgctgaag ccagcgatga
    11461 actgcgccac tactgcgatc gtctggatct cgatcccaac cgactatttg aacttgaaca
    11521 gcgcatctca aaacagattt cgctggcacg taaacatcac gtcagccctg aggcattgcc
    11581 acagtattac cagtcgctac tggaagaaca gcagcaactg gacgatcagg ccgactcaca
    11641 agaaacgctt gcgctggcgg taacgaaaca tcatcagcag gcactggaaa tcgcgcgcgc
    11701 attacaccaa caacgccagc aatatgcaga agaacttgca cagctgatca ccgacagtat
    11761 gcatgcgctc tcaatgccgc atgggcagtt tacgatcgat gttaaatttg acgagcatca
    11821 cctgggcgct gacggtgccg atcgtattga gtttcgggta accaccaacc caggtcagcc
    11881 aatgcagcct attgccaaag tcgcatccgg tggtgaattg tcccgcatcg cactggcaat
    11941 ccaggtcatc acggcgcgta aaatggaaac cccggcactg atttttgatg aagtggatgt
    12001 agggattagc ggtccaacag cggcagttgt cggcaaactg ctgcgtcaac tcggcgaatc
    12061 aactcaggtg atgtgtgtta cccacctgcc acaagtcgcg ggatgtggtc atcaacacta
    12121 ttttgtcagc aaagaaaccg atggtgcgat gacagaaacg catatgcaat ccctgaataa
    12181 aaaagcgcgg ttacaagagc tggcgcgcct gcttggtggc agtgaagtca cacgtaatac
    12241 actggcgaat gcgaaagaac tgcttgcagc gtaaactttt ttcctgcttc acggtcagag
    12301 taaacagcaa aacgccgtaa gaccggaaag caaaaggttt taaagtgatg aaaggtctat
    12361 tatcatcggc atattacaga tgagccacgt actgctcggg cccgaaaagg aatcaaatca
    12421 ctatgcgctg taaaacgctg actgctgcag cagcagtact attgatgttg accgcaggct
    12481 gttccactct ggagcgagtg gtttaccgtc ctgacatcaa ccaggggaac tatctgaccg
    12541 ctaacgacgt atccaaaata cgtgttggca tgacgcaaca acaagttgcg tacgcattgg
    12601 gtacaccgct gatgtccgat ccatttggta cgaatacctg gttctatgtc ttccgccagc
    12661 aaccaggtca tgaaggtgta acgcagcaaa cgctgacgct gacctttaac agtagcggtg
    12721 tgttgaccaa tattgataac aaacctgcgc tgagtggtaa ctaataataa agttgctctc
    12781 aaagacgtta aaaaaggtgc tcaatgagca ccttttttct gtctgttatt tattcgctga
    12841 tttttctgct cgttgcctgc gaagctcttt cggatcggca atgagagggc gataaatctc
    12901 cacccgatcg ccatcatgca cactatcgct tagttttgcc ggacggctgt aaatgccgac
    12961 tttgttttta gttaaatcga tatcggtacg caattccagc aagccactgg cgcgaatagc
    13021 ttcttcaacc gtcgcgccct cctgcagcgt cacgcgctgc aggtactgct tctcaggtag
    13081 cgcataagcc acctcaacgg caattttacc tggcactgta aacctctttc gcacgaaccg
    13141 taaaagcctg gaccatatta gacgccagct ctttaaacac gcgaccaaag gcgagttcaa
    13201 tcaacttatt ggtaaactca aagtcgagat gaaactcgat acgacacgcc tcctggctca
    13261 gcggcgtaaa cttccatcca ccaatcaatt tcttgaacgg cccatccacc agattcataa
    13321 gaatactttg gttactggtc aactggttgc gggtagtaaa cgttttgctg atcccagcct
    13381 tagagacatc taccgccgca gtcatctgcc caggagtgga ctccagaatc cgacttccgg
    13441 tacaacccgg caaaaactga ggataagact gaacgtcatt cactaactga tacatttgct
    13501 ccgcgctgta gggtaccagt gcggtccggc taatctgagg cataacaatt tccatcaaca
    13561 aaaatccaac aaataatatc atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg
    13621 taacatcggg ttcatgctaa gatagagcct tgtcccccgc aggattgata tggggtgttt
    13681 tcgatttcag attaccgatg attcacgacg cttatgacga agaaaaaagc acataaacct
    13741 ggttcagcga ccatcgcgct taacaagcgc gcccgtcacg aatactttat cgaagaagag
    13801 ttcgaagcgg gacttgccct gcaaggctgg gaggttaaat ccctgcgcgc aggaaaagcc
    13861 aatatcagcg acagctacgt ccttctgcgt gacggagagg catttctgtt tggcgctaac
    13921 atcacgccaa tggccgtggc ctccacgcat gtggtgtgcg atcctacccg tacccgcaag
    13981 ttacttctca accaacgcga actggactca ttgtacggtc gcgtcaatcg agaaggctat
    14041 accgtagtgg cgctctccct gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc
    14101 gccaaaggta aaaaacagca cgataaacgt tcagatatca aagaacgcga atggcaggtg
    14161 gataaagcac gtatcatgaa aaacgcccac cgttaaacct gcactccaat tattgaccag
    14221 ttcctcaccg cgcctccctc tccggcggcg cgaatgaaca tcttattggc tatcacatcc
    14281 gacacaaatg ttgccatccc attgcttaat cgaataaaaa tcaggctaca tgggtgctaa
    14341 atctttaacg ataacgccat tgaggctggt catggcgctc ataaatctgg tatacttacc
    14401 tttacacatt ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg
    14461 aggggcggtt ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga aaactacgct
    14521 ttagcagctt aataacctgc ttagagccct ctctccctag cctccgctct taggacgggg
    14581 atcaagagag gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt
    14641 aaaacttaat caggctagtt tgttagtggc gtgtccgtcc gcagctggca agcgaatgta
    14701 aagactgact aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc
    14761 gccagctcca ccactttaag aaggactaca accggacagt agcaataaat acagccactt
    14821 acggacacaa gccagacagt agtcggactg aaaaaagaca aaaatataca cgtgaaatgc
    14881 acgtgcactt ctaaaaaccc ctagttatca ccgactgggg tttttcaatt tgtaactaag
    14941 ggtaacaaaa acacccccgt ttttttcatc tcactcacct tgatactgtt tatttgtaca
    15001 gttaaaaata atactgtata caaacacagt atagaggaac ttttatgcgt attgagatct
    15061 gcatagccaa agaaaaaatg actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag
    15121 aattaacccg acgcatcagt aaacgttatg acgatgtaga ggtgatcgta aaagccacca
    15181 gcaacgatgg cctttctgtt acgcgcaccg ccgataaaga ttctgcaaaa acttttgttc
    15241 aggagacgct gaaagatacc tgggagtctg ctgacgagtg gtttgttcgc taataaacac
    15301 gtaaatcggt aacggctgga aatcattcaa tactcgcact atcgaaagtt caccagccaa
    15361 ccgcgacacg ctcttacata cgaagtgccg cgctttcctt aataattttt tagcagtact
    15421 gtgtaaatga tgagcgacct aatccatgca tgacgcgtag tgcctattgt gcatctttct
    15481 gcgtctcttt ttactggacc caagccaatg atgctggcga taccattccc gccaggccca
    15541 cttacaacag taaaattcgg ggctctgcct ttacatacaa tacaatgttg cacgattgcc
    15601 catcatcaac tccgttaata tctatgagta gtaaccctat gtaactgtaa agacgaatca
    15661 gcgctaattg tgtctgtcca catccccaaa tgtaaagata taaggagcac catgatacag
    15721 ttggatctga tttacatcct gtaaattctc gtcgatagcc acccctggaa aatcatgttg
    15781 ctggataaat ccgtatccta tgttagacac agcggaatac tctgtgaaat caggactcgc
    15841 ctcatatcgg tcactgatcg tacccagtct ttcgaagaaa gccctctcat tcttttcatg
    15901 cctcatggca gcctgtcgaa gggcattcag gttacgtagt ctaagatgag cttctcgttc
    15961 tggctctgca tatcctttga agtcgggaac actccatccc agctcttgag cgacacgacg
    16021 tgcgagaatc tcggtgggtc ctagctctat attactatct ccagatggat cgctggcccc
    16081 agtaacatga tgaataatct cgtgaatcag tccttcctgc cacgatggca tctcataaga
    16141 atcagtatct ggcgcaacac taaaactgac ataaggctct tcgttctcgt tttctcctgc
    16201 ctcgcaaatg gggagaattg gttcttgccc gacatcgtat ccatataatt cgttacaggt
    16261 tagtgattga atatcatcaa ttttgatagc agattctccg ttaagctcgt attcgtttct
    16321 gtatttaatg caaccaatgt gtacctcctc attatgaatg ccatagctta cggcatcacg
    16381 aaatgtttgt gatcggctca acgcatcaag tacagtgttg ccaatcatat cgaccgtatg
    16441 ctgatcgatg agcctgctac ggctgtcatg gacggcgcga attacacttt cgtatacgtt
    16501 gcttaaatct gctgcggaaa gcggtattcg cttacctata tccaaaacgt aatccgcata
    16561 ggcattttca gcacgattag gagcaacggc agcagagtat ccagcagggg caaaaaagtt
    16621 gaagccaggt tggagaacgg gaattttcat attgatactt aatatggttt tatatatttc
    16681 agtgccacca attcactagc ttacaaccag aaactaaaaa aacgtcaggg cgttgataat
    16741 aatgaatacc aactttctgc ccatttatag taatatcttt cgttatcagg ttttgtctgg
    16801 cagcttcacg taatatttca atactctgca aagcaacatt tgatgaaatg ctggatgtta
    16861 tcaatggcat atgtcattta ccccaacaag actaaatttt agaaaaaaca actgccggga
    16921 caactttcgc cccctgtaac cccaaataag atgtcagatt tatcggcatt aatttatctc
    16981 cctgttgaat tagctcctct atcatctact taacaaatat cccccggaca ttgcaaca
//
LOCUS       DAZFYC010000107        16812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFYC010000107 DAZFYC010000000
VERSION     DAZFYC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.107"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..102)
                     /locus_tag="ACHKW5_003858"
     CDS             complement(<1..102)
                     /locus_tag="ACHKW5_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513153.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKEN"
     gene            complement(230..2191)
                     /gene="topB"
                     /locus_tag="ACHKW5_003859"
     CDS             complement(230..2191)
                     /gene="topB"
                     /locus_tag="ACHKW5_003859"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO7513154.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2196..3239)
                     /gene="selD"
                     /locus_tag="ACHKW5_003860"
     CDS             complement(2196..3239)
                     /gene="selD"
                     /locus_tag="ACHKW5_003860"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO7513155.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3356..3907)
                     /gene="ydjA"
                     /locus_tag="ACHKW5_003861"
     CDS             complement(3356..3907)
                     /gene="ydjA"
                     /locus_tag="ACHKW5_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO7513156.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4068..5924
                     /gene="sppA"
                     /locus_tag="ACHKW5_003862"
     CDS             4068..5924
                     /gene="sppA"
                     /locus_tag="ACHKW5_003862"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO7513157.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6091..7107
                     /gene="ansA"
                     /locus_tag="ACHKW5_003863"
     CDS             6091..7107
                     /gene="ansA"
                     /locus_tag="ACHKW5_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO7513158.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETICKAMSQNLRGEL
                     TPDD"
     gene            7118..7759
                     /gene="pncA"
                     /locus_tag="ACHKW5_003864"
     CDS             7118..7759
                     /gene="pncA"
                     /locus_tag="ACHKW5_003864"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO7513159.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7920..8192)
                     /gene="yeaC"
                     /locus_tag="ACHKW5_003865"
     CDS             complement(7920..8192)
                     /gene="yeaC"
                     /locus_tag="ACHKW5_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO7513160.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8234..8647)
                     /gene="msrB"
                     /locus_tag="ACHKW5_003866"
     CDS             complement(8234..8647)
                     /gene="msrB"
                     /locus_tag="ACHKW5_003866"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO7513161.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8989..9984
                     /gene="gapA"
                     /locus_tag="ACHKW5_003867"
     CDS             8989..9984
                     /gene="gapA"
                     /locus_tag="ACHKW5_003867"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7513162.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10068..10952
                     /gene="yeaD"
                     /locus_tag="ACHKW5_003868"
     CDS             10068..10952
                     /gene="yeaD"
                     /locus_tag="ACHKW5_003868"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO7513163.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(11003..11857)
                     /gene="yeaE"
                     /locus_tag="ACHKW5_003869"
     CDS             complement(11003..11857)
                     /gene="yeaE"
                     /locus_tag="ACHKW5_003869"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO7513164.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11947..12693)
                     /gene="mipA"
                     /locus_tag="ACHKW5_003870"
     CDS             complement(11947..12693)
                     /gene="mipA"
                     /locus_tag="ACHKW5_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO7513165.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13129..15063
                     /gene="yeaG"
                     /locus_tag="ACHKW5_003871"
     CDS             13129..15063
                     /gene="yeaG"
                     /locus_tag="ACHKW5_003871"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO7513166.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15176..16459
                     /gene="yeaH"
                     /locus_tag="ACHKW5_003872"
     CDS             15176..16459
                     /gene="yeaH"
                     /locus_tag="ACHKW5_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO7513167.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            16606..>16812
                     /locus_tag="ACHKW5_003873"
     CDS             16606..>16812
                     /locus_tag="ACHKW5_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7513168.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLIL"
BASE COUNT         4172 a         4323 c         4095 g         4222 t
ORIGIN      
        1 attttcttta atcatccgtt caaaattact attaaatatt tcccagccat taaaagaata
       61 cttctcgctc ccaggatggt tttgtaataa aacttttttc atcacattcc ctgttattac
      121 atactttaga ggcgataatt ttatccatgc aaaaaaaata tccaacgaat tttcttgatc
      181 cgctggatat ttcagaatat gactcgaata gcacgaaaga ttcgctcgct tacgctatcg
      241 ccccgcttcc ggcttcatct gctggcggac tttttttcgc actacgttta cgcggtgcag
      301 cctttttctt atcagcactg ccaccactgc ccggagccac aatgccgcga aactgccgca
      361 ccggcgtacg tttggcttga tcaataagct gatatagcgt ccccaccagc ggctgcataa
      421 agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg
      481 tcatgtccgg tcgcgtagcc atttctggca gcgaatggaa taacgctttt ccggcgtcgg
      541 tggagtggat atagcgccct tttttggtga ggaaaccacg cttgaacaac agttcaataa
      601 tcccggcacg cgttgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat
      661 ctttgtcctg cacaaagcgc gcaatcccgg tcatcgccga aagcagtgtt gcatcggtaa
      721 aatggcgcgg cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat
      781 cgcctttcgc caccacaggc aatggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc
      841 ctaacagcgc gcgccaacct gcttcagcaa gaaaacgcgc tttagcgaca aatttgcctt
      901 tggcaatgtc cagttcgata acacacttgc ggaacactgc atccgggcag aactgcatca
      961 gatactgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag
     1021 aactccttgc ggtcggaatg atggcgtggt gcgcatcgac ctttttgtca tcccaacagc
     1081 ggttgcgtat atctggatct accactggct gcggcaacag atctggtgca tgaacgctga
     1141 tggcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag cgacaatcgg
     1201 aacgcggata agtgattaac ttgtgtgttt cgtacagctt ctggcagata tcaagcacgt
     1261 tctgcgcgct cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaacg
     1321 gcagcggcgc ggattctgat tcccgtttat cgttatagct ggtgacaata gccggttgac
     1381 cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc ccttcttcat
     1441 cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag
     1501 gtgtcacgat atgcgctttg acttcaaaga agtctttcgc cacgaagttt tcaatctctt
     1561 catcgcggcg caccaccagc ccgagcacgg gtgtctgcac gcgtcccacg gaaagtacgc
     1621 cctgataacc ggcattgcga ccgagaatgg tatacgcacg ggtcatattg atgccgtaca
     1681 gccagtcggc acgcgctcgc gccagcgcag aaacgcacaa tggcacaaac tcactgttgg
     1741 aacgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac
     1801 gctgcacctg ctggcgcttt tccggtgcca gttgcagata gtccagcact tcatccacca
     1861 gcaattgccc ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct tcatgcagga
     1921 agcgtttgat gacgttaagt tgtttggtca cggagggtcg gggctgtaat tgccactttt
     1981 ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg
     2041 gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact
     2101 cgataaagcc atcgccttta cggtgcggtt tgggcaggac atcagcaatg gcgcgcgcca
     2161 gactcggttt ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga
     2221 ccgccgcgcg ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct
     2281 gtagttttaa cctcattttc tgcttccggc atgaccgcca gcagcaaacc gccagaagtt
     2341 tgcggatcgc acagcagatc gcgcacttca cgcggcattt cacccatcag atgaccgtag
     2401 ctggcaaagt tacgttcggt gccgccaggc actgcgccca acttaatgta ctcttcaacc
     2461 cccggcaatt ttgggatcgc gtcatagtca acgcgcgcct gcacacctgc gccctgacac
     2521 atttcgctca aatggcccag cagaccaaaa ccagtaacgt cggtcatcgc ttttacgcct
     2581 tcgatgttgg caaaggacgc acctgcgatg ttcatccggc acatcacttc cgtcgccagt
     2641 ccctgatgtt ctggtttcaa cagtgatttt ttctcagccg tggtaagaac gccgatcccc
     2701 agcggtttcg tcaggaacag tttgcatccg gcttgtgcgg tactgttttt cttcacccgc
     2761 tcggtcggta cgatccccgt taccgccaga ccaaaaatcg gctccggcgc atcgatggag
     2821 tgacccccag ccagcgcaat acccgcctga cggcatgcat agcgtccacc ttcggttact
     2881 tcgcgggcaa tttctgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata
     2941 atcggtttgc cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca
     3001 aaatcgaaag gattatcaac gatcggcata aagaagtcgg tggtactgat aacgctggtg
     3061 ccattgccca gatcgtacac cgccgcatcg tcgcgggtct cattacccac aagcaaattc
     3121 ggatcaacaa acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt
     3181 ttacagccgc aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg
     3241 gacatctcct gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga
     3301 gagtgtctga attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga
     3361 aataagttac aaacggcgtc gggtccggga cgttaatcga cgtagatgct ttcaactgcg
     3421 gcgtgccgag gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac
     3481 gcactaccgg actttcagtt aatgcgccac tgcgccagat gccgccaaat ccttgggcaa
     3541 cggctgccat ttgcatcgcc atgaccgcgc atccggcaga catttcctgt tcccatcgcg
     3601 ggactttatg attttcttcg catttcgcca ccaccgtgat gatgagcggt gcgcggaacg
     3661 gcgcattacg ggctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt
     3721 ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa tgccacggtt
     3781 gcatggactt atggtccggc gcacgcatac ccgcacgcag gatgttttgc agttgttcac
     3841 ccgttggcgc gggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg
     3901 catccatttg attaactcct gtcgtgatat ttattcacaa aattaacacg agagtggatt
     3961 ttgttacagc acagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc
     4021 acatctattg cgcctgtgac aggtgtgacc ttaagttggg agaatacatg cgaacccttt
     4081 ggcgatttat tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa
     4141 tggtacttaa cctgttcttt attttcctcg tactggttgg tgtggggatc tggatgcagg
     4201 tcagtggtgg tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg gacatttctg
     4261 gtgtgatcgt cgataaaccc gacagttctc agcggtttag taaattaagc cgccagctgc
     4321 ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc
     4381 gccaggcgaa ggacgaccgc aatatcaccg gtatcgtgat ggatctgaaa aacttcgcag
     4441 gcggcgacca gccgtctatg cagtacatcg gcaaagccct gaaagagttt cgtgacagcg
     4501 ggaaaccggt ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt
     4561 tcgccaataa aatttggctg tcaccgcaag gcgtggttga tctgcacggt tttgctacca
     4621 acggtctgta ctacaaatcg ttgctggata agctgaaagt ttccacccat gtgttccgcg
     4681 tgggtacgta taaatctgcc gttgaaccgt ttattcgtga tgatatgtca ccggcagccc
     4741 gcgaagctga cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg
     4801 ctaaccggca gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt
     4861 taaccaaaac cggtggcgat accgcgaaat atgcactgga aaacaagctg gtcgatgcac
     4921 tggcatcgag tgccgaaatc gaaaaagctc tgaccaaaga gttcggctgg agtaagactg
     4981 ataaaaatta tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg
     5041 gtgacagcat cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg
     5101 ggaatgttgg cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag
     5161 tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga
     5221 ttcgcgctga actggcagca gcccgggcag cgggtaagcc tgtggttgta tcgatgggcg
     5281 gcatggcggc atctggtggt tactggattt ccacgccagc taattacatt gtggctaacc
     5341 ccagcaccct gacaggttct atcggtatct tcggcgtgat caccaccgta gaaaatagtc
     5401 tggattcgat tggtgttcat actgatggtg tctcaacttc accgctggcg gatgtttcta
     5461 tcaccagggc actgccgccg gaagcgcagc agatgatgca attaagcatt gagaatggct
     5521 ataaacgctt tatcacgctg gttgctgatg cgcgtcattc gacgccggag caaattgata
     5581 aaatcgccca gggccacgtc tggaccggtc aggatgcaaa agctaacggg ctggtcgata
     5641 gtctcgggga tttcgatgat gcggttgcca aagcagcaga gctggcaaaa gtgaaacagt
     5701 ggcatctgga atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaacatgt
     5761 ctggttctgt ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgcttg
     5821 cctcggtagc ctctaccgtt aaaagtgaaa gcgacaagct ggccgcgttt aacgacccac
     5881 aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg tactgagtgg
     5941 ccgacagatc gtcggccaca ttatttttta cctcgacgaa tcctcttccc gctgtctcga
     6001 cccatatttc cttatccgca tagtatcagg tgcgctcctc ctgcctcacg tatatacttt
     6061 tgctctttcg atatcattca tatcaatatc atgcaaaaga aatcaattta cgtcgcctac
     6121 acgggcggga ccatcgggat gcagcgttcc gagcagggtt atattccggt gtcaggtcat
     6181 ctacaacgcc aactggcgct gatgccggaa ttccatcgcc cggagatgcc agatttcacc
     6241 attcatgaat atacgccgtt gatggattct tctgatatga cgccggaaga ctggcagcat
     6301 attgctgaag atattaaagc gcactatgac gactatgatg gttttgtcat tctacacggt
     6361 accgacacga tggcgtatac cgcctctgcg ctgtcgttca tgctcgagaa tctcggtaaa
     6421 ccggtcattg tgacagggtc acaaatcccg ctggctgagt tacgctctga cggacaaatt
     6481 aatctgctga atgcgttgta cgttgcggcg aattatccga tcaacgaagt aacgctcttt
     6541 ttcaataacc gattgtatcg cggcaaccgc actaccaaag cccatgccga tggttttgat
     6601 gcgtttgcct ctccaaacct tcctccgtta ctggaagctg gtatccatat tcgtcgtttg
     6661 aatacgccac ccgccccgca cggtgaaggg gaattaatcg ttcatccaat cacgccacaa
     6721 ccaattggcg tagtgacgat ttatccgggg atttctgctg atgtcgtgcg caattttctg
     6781 cgccaaccgg tgaaagcatt gattctgcgc tcctatggcg tgggtaatgc gccacaaaac
     6841 aaagccttcc tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt ggttaacctg
     6901 acacaatgta tgtccggtaa agtgaacatg ggtggttatg ccaccggtaa cgccctcgcc
     6961 catgccggcg taattggcgg tgcagatatg actgtagaag ctacgctaac caaactgcat
     7021 tacctgctga gccaggaact ggatactgaa accatttgca aggccatgag ccaaaacctg
     7081 cgcggcgaac tgacgccgga tgattaagga gactgcaatg ccccctcgcg ccctgttact
     7141 ggtcgattta caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg aaggtgacag
     7201 tacggtggat gtcgctaacc gccttattga ctggtgccag tcgcgcggtg aagcggttat
     7261 cgccagtcag gactggcacc cggcgaatca cggcagtttt gccagtcagc actgtgtaga
     7321 gccttatatg ccaggtcaac tcgacggttt gccacaaacc ttctggccag atcactgtgt
     7381 gcagaacagt gaaggcgcac aattacatcc gttactgaac caaaaagcga tcgcagcggt
     7441 gttccataaa ggcgaaaatc ctttagttga cagttacagt gccttttttg ataacggccg
     7501 tcggcagaaa acctctctcg atgactggtt acgcgatcat gaaatcgatg aattgatcgt
     7561 tatgggcctg gctactgact attgcgtgaa gtttaccgtg ctggacgcgt tacagttagg
     7621 ttataaagta aacgtgatta ccgatggttg tcgtggcgtg aatatccagc cccaggacag
     7681 tgcgcacgcg tttatggaga tgtcagcagc tggggcaacg ctatatacgc tggcagactg
     7741 ggaagagacg caggggtaat attagttgaa ttcatctctg gcctacattt cctcaatgac
     7801 attgtaggcc agataatact tgtcagtgtt gcatttaacc tacttgcagc taaaacacga
     7861 cgtcctaaca agtatatttg ccctccctta ataacaggct taaatattag ataactcatt
     7921 cacttaaaca tagcaatagg ctttgctgag ataccaaaat cttcttttaa ctgctgtttg
     7981 ctcttcatga ccatctgacc atttgtgtca attgtcatat gctgtgcttc ggtattatgg
     8041 cgggcttgcc acagcatcac cagttgcagg caattctctt tttgttcctc ggtcaacgca
     8101 acgccatcag gccatttccc cagttcaacg gcggtcgaca aacgctggta tacctcaggc
     8161 gtcatgctgt tgataatgtc atcaagattc ataatttttc cgctcctgtg gaataagttg
     8221 ctgaatcgtt ttttcaaccg ttgatttctt cgccgttttc gccatcggta aagcgtaaag
     8281 aggcagagtt aacacaataa cgttcgcccg ttggctgcgg cccgtcaggg aagacatgcc
     8341 ccagatgggc atcacagtta ccgcaacgaa tttctatgcg ctgcattcca tgtgacaagt
     8401 ctttgatata acgaatggat tcttcactca ccggttcgta gaaactgggc caaccacagc
     8461 cggaatcata cttggtttgg gaatgaaaca gcggggcatc gcagatcaaa cagtgatata
     8521 cgccgtcacg cttgttatgc agtaaacgac ccgtaaatgg cggctctgtc ccatgattct
     8581 gcgtcacgta aaactgcatc tcggacaaat tttttttcag ttcttctgcc gaaggtttat
     8641 tagccatttg ctcacatctc actttaatcg tgctcacatt acgtgactga ttctaacaaa
     8701 acattaacac caactggcaa aattttgtcc taaacttgat ctcgacgaaa tggctgcacc
     8761 taaatcgtga tgaaaatcac atttttatcg taattgccct ttaaaattcg gggcgccgac
     8821 cccatgtggt ctcaagccca aaggaagagt gaggcgagtc agtcgcgtaa tgcttaggca
     8881 caggattgat ttgtcgcaat gattgacacg attccgcttg acgctgcgta aggtttttgt
     8941 aattttacag gcaacctttt attcactaac aaatagctgg tggaatatat gactatcaaa
     9001 gtaggtatca acggttttgg ccgtatcggt cgcattgttt tccgtgctgc tcagaaacgt
     9061 tctgacatcg agatcgttgc aatcaacgac ctgttagacg ctgattacat ggcatacatg
     9121 ctgaaatatg actccactca cggccgtttc gacggtaccg ttgaagtgaa agacggtcat
     9181 ctgatcgtta acggtaaaaa aatccgtgtt accgctgaac gtgatccggc taacctgaaa
     9241 tgggacgaag ttggtgttga cgttgtcgct gaagcaactg gtctgttcct gactgacgaa
     9301 actgctcgta aacacatcac cgctggtgcg aagaaagtgg ttatgactgg tccgtctaaa
     9361 gacaacactc cgatgttcgt taaaggcgct aacttcgaca aatatgctgg ccaggacatc
     9421 gtttccaacg cttcctgcac caccaactgc ctggctccgc tggctaaagt tatcaacgat
     9481 aacttcggca tcatcgaagg tctgatgacc accgttcacg ctactaccgc tactcagaaa
     9541 accgttgatg gcccgtctca caaagactgg cgcggcggcc gcggcgcttc ccagaacatc
     9601 atcccgtcct ctaccggtgc tgctaaagct gtaggtaaag tactgccaga actgaatggc
     9661 aaactgactg gtatggcgtt ccgcgttccg accccgaacg tatccgtagt tgacctgacc
     9721 gttcgtctgg aaaaagctgc aacttacgag cagatcaaag ctgccgttaa agctgctgct
     9781 gaaggcgaaa tgaaaggcgt tctgggctac accgaagatg acgtagtatc taccgatttc
     9841 aacggcgaag tttgcacttc cgtgttcgat gctaaagctg gtatcgctct gaacgacaac
     9901 ttcgtgaaac tggtatcctg gtacgacaac gaaaccggtt actccaacaa agttctggac
     9961 ctgatcgctc acatctccaa ataagttgag atgacactgt gatctaaaaa gagcgacttc
    10021 ggtcgctctt ttttttacct gataaaatga agttaaagga ctgcgtcatg attaagaaaa
    10081 tttttgccct tccggtcatc gaacaaatct cccctgtcct ctcccgtcgt aaactggatg
    10141 aactggacct cattgtggtc gatcatcccc aggtaaaagc ctcttttgca ttacagggcg
    10201 cacaccttct ctcgtggaaa cctgcgggtg aagaagaagt tctgtggttg agcaacaaca
    10261 caccgttcaa aaatggcgtc gctattcgcg gtggcgtacc ggtttgctgg ccgtggtttg
    10321 gtccggcggc acaacaaggt ctgcctgcgc acggttttgc ccgcaacctg ccgtggacgc
    10381 tgaaatcaca tcgtgaagat gctgatggcg tagcgctgac ttttgaattg acgcaaagcg
    10441 aagagacgaa aaaattctgg ccgcacgact ttacgctgtt agcgcatttc cgcgtgggta
    10501 aaacctgtga aatcgatctt gaatcacatg gcgaatttga aaccacctct gccctgcata
    10561 cctactttaa cgtgggtgat atcgctaagg taagcgtcag tgggctgggc gatcgcttca
    10621 ttgataaagt aaatgacgcg aaagaagatg tactgaccga cggtattcag accttccctg
    10681 accgtaccga tcgcgtgtat ctgaatccgc aagattgcag cgtgattaat gatgaagcgc
    10741 tgaatcgtat tatcgccgta ggccaccagc atcatctgaa cgttgtcggc tggaacccgg
    10801 gaccggcgct ttcagttagc atgggcgaca tgccggatga tggctacaaa acatttgttt
    10861 gtgtagaaac ggcttacgct tcagaaacgc aaaaagtgac caaagagaaa cctgcacatc
    10921 tggcgcaatc cattcgcgtt gcgaaacgtt aatttgcgtt aatgttgtgt gccgggtgcg
    10981 atgcatccgg cacgtatcaa catcacacca tatccagcgc agtttttcct tttggtgccg
    11041 gatacgcttt atccagcatg attaattccg ctgacgaaag ttcgacctca agcacagccg
    11101 cattctgttg gacatgggca atcgtggccg cttttggaat cgccatcaca ccctgatggc
    11161 tgattaccca cgccaacaat acttgtgccg cgctgatatt gtgagcatgt gcaatttcgt
    11221 tgactaccgc gttttttaac agtccattgc gcaaccgccc ggcctgggct aatggactgt
    11281 aagccattac cggcatctgc tgttgctggc accaggggag tagatcgtac tcaatccctc
    11341 gtgaaccgag atggtaaagc acctgattag tggcacactg atttcccccc ggcagctgcc
    11401 agagttcctg catatcagca taatcaaggt tagaaacgcc ccagcggcgg atttttccct
    11461 gggcgatcaa tttttccatc gctgccacag tctcttcaaa agcgaaactg ccagaccagt
    11521 gtaataagta gagatcgaga taatcagtat tgagacggcg taaactggct tcgcatgcat
    11581 ttatcgcttt ttgcccgcca gcattccacg gatagacttt agagacgaga aagacgttat
    11641 cgcgcagatc ggttaatgct tccccaacca ccttttcggc accgccatcg gcatacattt
    11701 cggcggtatc aatgagggtt aaaccgagct caatgcccgc gcgtagtgca gcaacttctg
    11761 ttttgcgctg actggcatct tcgcccatat accatgttcc ctgccctatg gctggcagtg
    11821 agacatcgcc actaaattga atcatttttt gttgcattgt ttcctcccag gtattgcacc
    11881 accgtaatgc aaaacagggc gtgacgccct gtttttatgc acaaaatgcc ctggaaagat
    11941 gcattatcag aatttgtagg tgatcccggt agaaatcagg ccagtccagg atttatccac
    12001 catcgggctg tcagtaactt catcagacag acgggtatag cgcgcggtac cgtaaacact
    12061 ccagtcgccg aggaagttgt agctggcgct cagctccagg taagggctcc agctgtcgtt
    12121 cgggttatag ccacgcagac cgctgcgagc ggactctttg cgcgatacgc cataatagta
    12181 ttcgttctgg ttttcgctgt tccactgcac accaataccc ggagtcacgg tcaggccacc
    12241 gttggtgtaa cgatacaacc aggccatatc ccagacaatg ccgttgctgt tatccagggt
    12301 atcgccagcc agggtggtac gcaggtaacc gtactgggta aagtgagcat aagacagacc
    12361 agccatcatg gtgctcttac ggtcatccag gtgacgcatt tggtgatcgc cactgtcttt
    12421 cgctttgaag taaagcggcg accagtaagc ggtaattgaa agtttatccg ttgcgtcatt
    12481 ccacaggtag taaccaccac ctaagccacg gaaccagaag ttatcgcctt catagttgat
    12541 taccggtact gggtaaacat cggtatcgta atctttatat gggtgctcaa cgacacctac
    12601 gcctgcgccc agggaaaatt taccttcagc gtgcgctacg cctgcagacg ttgcgataag
    12661 cactccaagt gccagaagtt tgagtttggt cacaattaat cattccttaa acaaatgttt
    12721 agcgggcgac aaagtttacc cgtcaataca tcgaaaccca acctttttac gttttcattt
    12781 tttaaagtaa ctgtttaatt ttcctgacgc ggatgacacc gcgcttacag ccaaatgaat
    12841 ttagcgttac tggctaccct ggtctttaca ttaattatgc aaaatttatg gatgagttgt
    12901 tgatatgcca ttgaaattaa gaaagccgtg caggcaagtt ttccatttgc catctacgct
    12961 taattttgaa ggtgtatcac cgggcacgtt gttctcatcg tcgataaaat ggcatgagag
    13021 ttgctgtgtt ttagcaagag acgtcgttca gtttacctct tccgggagcc tctactattc
    13081 atatgaacgg ctcttaacct gtgctaaaaa acgaaaggac ggcataccat gaatatattc
    13141 gatcactatc gccagcgata tgaagctgcc aaggacgaag agttcacact gcaggagttt
    13201 cttaccactt gtcggcaaga tcgcagtgct tatgccaacg cggctgagcg gctattgatg
    13261 gctatcggtg agcctgtcat ggtcgataca gcccaggaac ccagactttc tcgactcttt
    13321 tctaaccggg tcattgcacg ttatccggcg tttgaagagt tttacggcat ggaagacgcg
    13381 attgaacaga ttgtctctta tctgaaacac gcggctcagg ggctggaaga gaagaaacaa
    13441 atcctgtatc tgctggggcc tgtgggtggg ggtaaatcat cgcttgctga gcgactgaaa
    13501 tcattaatgc agctcgtacc gatttatgta ttgagcgcca acggtgagcg tagcccggtc
    13561 aacgatcatc cgttctgtct tttcaatccg caggaagatg ctcagattct ggaaaaagag
    13621 tatggcattc ctcgccgtta tctcggcacc atcatgtcgc catgggcggc aaaacgcctg
    13681 catgaatttg gtggcgatat cactaagttc cgggtagtga aggtctggcc gtcaattctg
    13741 caacaaattg ctatcgccaa aacggaaccc ggtgatgaga acaaccagga catctccgcg
    13801 ctggttggga aagtcgatat tcgtaaactc gaacactacg cgcagaatga cccggacgcc
    13861 tacggctatt ccggtgcgct gtgccgcgcc aaccaaggga tcatggagtt cgttgagatg
    13921 tttaaagcac cgataaaagt gctgcatccg ctgttaaccg ccactcagga aggtaactac
    13981 aacgggacgg aaggtatctc cgccctgccg ttcaacggga ttatcctcgc ccactcgaac
    14041 gaatccgaat gggtcacttt ccgtaataac aaaaacaacg aagcctttct cgaccgtgtt
    14101 tacatcgtga aggtgccgta ttgcttgcgc atttccgaag agatcaaaat ctacgagaaa
    14161 ttgcttaatc acagtgaatt gactcacgcc ccatgcgccc ctggcacgct cgaaacactg
    14221 tcacgttttt ccattctttc gcgcctgaaa gagccagaaa actccagcat ttattcaaag
    14281 atgcgggttt atgatggcga aagtctgaaa gacaccgatc ccaaagccaa gtcgtatcag
    14341 gaatatcgtg actacgccgg tgtcgatgaa gggatgaacg gtctgtcgac gcgttttgcg
    14401 tttaagatcc tctcccgcgt gttcaacttc gatcatgtag aagtggcagc aaacccggtc
    14461 catctgttct acgtcctgga acagcagatc gagcgcgagc agttcccaca agagcaggca
    14521 gaacgctatc tggagttcct gaaaggttat ctgatcccga aatatgccga gtttatcggc
    14581 aaagagatcc agacggccta ccttgaatcc tattccgaat atgggcaaaa cattttcgac
    14641 cgttatgtta cctacgcgga tttctggatt caggatcagg agtatcgcga tccggatacc
    14701 gggcagctgt ttgaccgcga gtctcttaac gccgagctgg agaaaatcga gaaaccggcg
    14761 gggatcagta atccaaaaga tttccgcaac gagattgtta acttcgtact gcgcgccaga
    14821 gcgaataaca gcggacgaaa tccgaactgg accagctatg aaaaactgcg cacggttatc
    14881 gagaagaaaa tgttctccaa taccgaggag ctgttgccgg ttatctcgtt taacgccaaa
    14941 acgtcaaccg acgagcagaa gaaacacgac gactttgtcg accgtatgat ggaaaaaggc
    15001 tacacccgta aacaggtgcg tttactgtgc gaatggtatt tgcgcgtacg taaatcgtct
    15061 taacaaccct ggcccggtac acatgctacc gggcctacaa cgacagcgaa ccgtgggcca
    15121 gagaaatggc aacacaggcg tagcatacag ttggcaaatg tagtacgggg ggcatatgac
    15181 ctggtttatt gaccggcgtc tgaacggcaa aaacaaaagc atggtgaatc gccagcgttt
    15241 tttacgccgt tataaagcgc aaattaaaca gtcgatctcc gaggccatta ataagcgttc
    15301 ggtgactgac gtcgacagcg gcgaatccgt atccattccc acggaagata ttagcgaacc
    15361 gatgtttcat caggggcgtg gcggtctgcg ccaccgcgtg catccgggca atgaccattt
    15421 cgtccagaac gaccgaattg aacgtcccca gggtggcggc ggaggttccg gcagtggaca
    15481 gggccaggcc agccaggatg gtgaaggtca ggatgaattt gtctttcaga tttcgaaaga
    15541 tgagtatctt gatctgctct ttgaagattt ggctttaccg aatctgaaac aaaaccaaca
    15601 acgccagctg accgaatata aaacgcatcg ggcgggttat accgctaacg gcgttccggc
    15661 caatatcagc gttgtacgtt ccttgcagaa ctcactggcg cgtcgcacag ctatgacggc
    15721 gggcaagcga cgggaacttc atgcgctgga agagaatttg gccatcatca gcaacagtga
    15781 acctgcgcaa ctgctggaag aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa
    15841 aattgaacgc gtccctttca ttgacacctt cgatttacgt tacaagaact acgagaagcg
    15901 gcccgatccc tccagccagg cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga
    15961 tcaatccact aaagatatgg ctaagcgttt ttatattctg ctgtatctgt tcctcagcag
    16021 aacgtataag aacgtggaag tcgtatacat ccgccatcat acccaggcga aagaagtcga
    16081 tgaacatgag tttttctact cgcaggaaac aggcggcacc attgtttcca gcgccctgaa
    16141 actgatggat gaggtagtga aagagcgtta taacccggca cagtggaata tttacgctgc
    16201 acaagcatcg gacggcgata actgggccga tgactctccg ctttgccatg aaatcctggc
    16261 gaaaaaatta ttacctgttg ttcgttatta cagctatatc gaaattaccc gtcgtgcaca
    16321 tcagacattg tggcgagaat atgagcatct gcaatctact ttcgacaact ttgcgatgca
    16381 gcacatccgc gaccaggatg atatttatcc ggtattccgt gaactgtttc ataaacaaaa
    16441 tgcaacagct aaagactaaa actatcagcc gggtcattat cgcctggctg atttttagct
    16501 tactgtaaat tatctcattt attacataca ttagcttaca atcgctttaa atatgacagc
    16561 ataaccttta cataatttag ttccagaaaa caatcattcg gaaaaatgat tcagtcaaca
    16621 cgtatttcca tggggttatt ctttaaatat tttttatcgt taacgaaaat tgatcctggt
    16681 caaaactata tatctctgcc atcaataaaa tccagcactc acattgctct cctttttatg
    16741 gtttctatgg gtacacaaaa attaaaagct caaagctttt ttattttcag tttattgctg
    16801 acgttaattt ta
//
LOCUS       DAZFYC010000108        16777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFYC010000108 DAZFYC010000000
VERSION     DAZFYC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.108"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(148..2415)
                     /gene="adiA"
                     /locus_tag="ACHKW5_003874"
     CDS             complement(148..2415)
                     /gene="adiA"
                     /locus_tag="ACHKW5_003874"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO7513169.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2614..3522)
                     /gene="melR"
                     /locus_tag="ACHKW5_003875"
     CDS             complement(2614..3522)
                     /gene="melR"
                     /locus_tag="ACHKW5_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO7513170.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRAHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3805..5160
                     /gene="melA"
                     /locus_tag="ACHKW5_003876"
     CDS             3805..5160
                     /gene="melA"
                     /locus_tag="ACHKW5_003876"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO7513171.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5263..6684
                     /gene="melB"
                     /locus_tag="ACHKW5_003877"
     CDS             5263..6684
                     /gene="melB"
                     /locus_tag="ACHKW5_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO7513172.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6823..7452)
                     /gene="yjdF"
                     /locus_tag="ACHKW5_003878"
     CDS             complement(6823..7452)
                     /gene="yjdF"
                     /locus_tag="ACHKW5_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO7513173.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7575..9221)
                     /gene="fumB"
                     /locus_tag="ACHKW5_003879"
     CDS             complement(7575..9221)
                     /gene="fumB"
                     /locus_tag="ACHKW5_003879"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7513174.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9299..10639)
                     /gene="dcuB"
                     /locus_tag="ACHKW5_003880"
     CDS             complement(9299..10639)
                     /gene="dcuB"
                     /locus_tag="ACHKW5_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO7513175.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11210..11929)
                     /gene="dcuR"
                     /locus_tag="ACHKW5_003881"
     CDS             complement(11210..11929)
                     /gene="dcuR"
                     /locus_tag="ACHKW5_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO7513176.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11926..13557)
                     /gene="dcuS"
                     /locus_tag="ACHKW5_003882"
     CDS             complement(11926..13557)
                     /gene="dcuS"
                     /locus_tag="ACHKW5_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO7513177.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13738..13968
                     /gene="yjdI"
                     /locus_tag="ACHKW5_003883"
     CDS             13738..13968
                     /gene="yjdI"
                     /locus_tag="ACHKW5_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO7513178.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13980..14252
                     /gene="yjdJ"
                     /locus_tag="ACHKW5_003884"
     CDS             13980..14252
                     /gene="yjdJ"
                     /locus_tag="ACHKW5_003884"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7513179.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14479..14775
                     /gene="ghoS"
                     /locus_tag="ACHKW5_003885"
     CDS             14479..14775
                     /gene="ghoS"
                     /locus_tag="ACHKW5_003885"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7513180.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14803..14976
                     /gene="ghoT"
                     /locus_tag="ACHKW5_003886"
     CDS             14803..14976
                     /gene="ghoT"
                     /locus_tag="ACHKW5_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO7513181.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15095..16612)
                     /gene="lysS"
                     /locus_tag="ACHKW5_003887"
     CDS             complement(15095..16612)
                     /gene="lysS"
                     /locus_tag="ACHKW5_003887"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7513182.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDVEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         4105 a         4002 c         4263 g         4407 t
ORIGIN      
        1 ggcaaaaaaa agcgcggggt ggggccgcgc aaaaagtatg acatttttgt ttacagcaat
       61 ttttatgaaa acgttgccat actcaaaagg cctaaggcca ggcggcgtaa tgttatttaa
      121 acaattacgc cttcagcgga atagtggtta cgctttcacg cacataacgt ggtaaatacc
      181 gtcaataatt tcagtccctt cagtttcgtg ttcaaatcca gggaaatggt ggtcccagga
      241 ttgcagcgag cgtaaataac ttacttgcgg actgttttta tcgccgaagt tttcaccaga
      301 cagcagcatc gggattcctg gcggatacgg gataactgag tttgccgcga tgcgtcctgg
      361 cagattttca atggatacca gttcgacatt gttgtcgaca atcgcgttgt acgcttcacg
      421 cggggtgact tccgccaccg gcaggccgga ataggcttcg ttcaaccgtg cgccagggtt
      481 gttttctttc agccaggcaa acatggtgtc acccagatcg tgaatcccca tgttcgcgta
      541 agtgtcagga tattgttcaa caagttccgg catcacctgc gccagcggtg tgttggcgtc
      601 atagtggcgt ttgaaggagc aaagggtgtt aaccagagtt ccccatttcc cacgggttac
      661 gcccatagag aacaggaaca taatttggaa gtcagtggtg cgggtaggta caatgccgtg
      721 gcgaccaagc caggcagtga ccagcgccgc cggaacaccg gtttcttcca gttcaccatc
      781 ttcacccatt cccggagcaa ggatgctgac tttaatcggg tcgagcatac tccagttatc
      841 cggaatatct ttgaagccgt gccagctttc gcccggatgc attacccagc agtcctgaac
      901 ggtggtcagc agtttggttg gtgcgtcagc aaagtcatag gttttgccgg tttgtgggtc
      961 ggtgacgact tctttgttcc acggtttgaa gaaccagcta ccgtcagcgg tgaactcttt
     1021 atatagccgc gccatcgcct gacggaaatc aaccgcttcg tcaatcactt cctgtgtcag
     1081 tgacaggccg ctgttgccgt ccatcatcga caccgccacg tcgttggatg cgcagatggc
     1141 atacagcggg gaggtggtgg catgcatcat gtaggcctgg ttgaagcggg agaagttaat
     1201 cgccccacga ccttcacgta catgaatata agaagcctgt gacagcgcat tcagcagttt
     1261 gtgggtggag tgggtggcga aaacggtagg accgttgtga tcgccaggtt cgccgcgcat
     1321 ggcatagtga tcggcataga tcgggttgaa acgtgcatag ccgtaccagg cttcgtcaaa
     1381 gtgcagacga tcggaggttt tttccagcag atcctgcgct tctttagcgt tataacacac
     1441 gccgtcatag gtgcagttgg tcaccacgca gtaagacggt ttttgcccgg ctttgtcttt
     1501 ggtcagcggg ctttcactga ttttcttctg caaggtttca ggttgcattt cctgcggata
     1561 gattggcccg ataatgccgt agcggttgcg gcttggcacc atatagaccg gtttcgcgcc
     1621 tgtcagcatc aaaccttgtt cgatggattt atggcagtta cggtcaacga ccacgacatc
     1681 gttatcggtc atgcaagcct gcatgatggt gcggttagag ccggaagtac cgacgactac
     1741 cgaccaggag cgatcggcac caaatacgcg tgcggcatat ttttcgcttt cgccaaatgc
     1801 gccagtatgg tcaagcaaag aaccgaggga agttcgttcg atgcccatgt cggtgcggaa
     1861 cagattttca ccatagtagt catggtagaa acgtccggcg ggtgttttgg taaaaccaac
     1921 gccgccctgg tggcctggcg ctgcccagga atattcatgg atgtcactat atttcatcag
     1981 cgcgctgaac agtggcggca acagctgctg gcggtagcgg gtcatcgcgg caacggcgcg
     2041 tccggcgata aagtcggcgg tatcttccag aatccaggcg aattcatcga caagctccag
     2101 caggtcgcga tccattgcgg cgagggcttt ttcccgatcg cccaacagga agaccggcac
     2161 gttttgttgg cgctcatgaa gcttaccgat caattgtctg acgttttgat gttcgtccgg
     2221 atgttccatt tgatagctga acatcaggca gtcaatggct tcgtttgaag agagaatggc
     2281 aaaaccatca tcaaaggagg tggatttaat cacggtaaca ttttgctggc ttaaagcatc
     2341 tgccagacgc tcaacggcgt taccgaccca ggtgtcttga tggagaaact cgctttcaac
     2401 aattaatact ttcatcattg cttacccggt tatgaaggaa tcttcgttgc gggcaagtat
     2461 cttccggcct tttcgggctg taaagcgcgt gaaaaacaaa catttttgaa atatttattt
     2521 ttataaacaa tgcgttatgt gattatttta aattattttc actgcttatt atcgacggct
     2581 aaactatttt tttggctgat actgatatcg tctttagccg ggaaacgtct ggcggcgctg
     2641 ttggctaagt ttgcggtatt gttgcggcga catgccgaca tatttgccga acgtgctgta
     2701 aaaacgacta cttgaacgaa agcctgccgt cagggcaata tcgagaatac ttttatcggt
     2761 atcgctcagt aacgcgcgaa cgtggttgat gcgcatcgcg gtaatgtact gtttcatcgt
     2821 caattgcatg acccgctgga atatccccat tgcatagttg gcgttaagtt tgacgtgctc
     2881 agccacatcg ttgatggtca gcgcctgatc atagttttcg gcaataaagc ccagcatctg
     2941 gctaacataa aattgcgcat ggcgcgagac gctgtttttg tgtgcgcgcg aggttttatt
     3001 gaccagaatc ggttcccagc cagagaggct aaatcgcttg agcatcaggc caatttcatc
     3061 aatggcgagc tggcgaattt gctcgttcgg actgtttaat tcctgctgcc agcggcgcac
     3121 ttcaaacggg ctaagttgct gtgtggccag tgatttgatc accatgccgt gagtgacgtg
     3181 gttaatcagg tctttatcca gcggccagga gagaaacaga tgcatcggca gattaaaaat
     3241 cgccatgctc tgacaggttc cggtatctgt tagttggtgc ggtgtacagg cccagaacag
     3301 cgtgatatga ccctgattga tattcacttt ttcattgttg atcaggtatt ccacatcgcc
     3361 atcgaaaggc acattcactt cgacctgacc atgccagtgg ctggtgggca tgatatgcgg
     3421 tgcgcgaaac tcaatctcca tccgctggta ttccgaatac agcgacagcg ggctgcgggt
     3481 ctgtttttcg tcgctgctgc acataaacgt atctgtattc atggatggct ctctttcctg
     3541 gaatatcaga attatggcag gagtgaggga ggatgactgc gagtgggagc acggttttca
     3601 ccctcttccc agagaggcga ggggagcgtc cgagcatcat gaggccgaaa actctgcttt
     3661 tcaggtaact taataccata aactcagatt tactgctgct tcacgcagga tctgagttta
     3721 tgggaatgct caacctggaa gccggaggtt ttctgcagat tcgcctgcca tgatgaagtt
     3781 attcaagcaa gccaggagat ctgcatgatg actgcaccca aaattacatt tatcggcgct
     3841 ggttcgacga ttttcgttaa aaatattctt ggtgatgtgt tccatcgcga ggcgctgaaa
     3901 acggcgcata ttgccctgat ggacattgat cccacccgcc tggaagagtc gcatattgtg
     3961 gtgcgtaagc tgatggattc agcaggggcc agcggcaaaa tcacctgcca cacccaacag
     4021 aaagaagcct tacaggatgc cgattttgtc gtggtggcat ttcagattgg cggttatgaa
     4081 ccttgcacgg tgactgattt cgaggtctgt aagcggcatg gtctggaaca aaccattgcc
     4141 gatacgttgg ggccgggcgg tattatgcgc gcgctacgta ccattccgca tctgtggcaa
     4201 atttgcgagg acatgacgga agtctgcccc gatgccacca tgctcaacta tgttaaccca
     4261 atggcgatga atacctgggc gatgtatgcc cgctatccgc atatcaaaca ggtcgggctg
     4321 tgccattcgg tgcagggaac ggcggaagag ctggcgcgtg acctcaatat cgacccagct
     4381 acgctgcgtt accgttgcgc aggtatcaac catatggcgt tttacctgga gctggagcgc
     4441 aaaaccgccg acggcagtta tgtgaatctc tacccggaac tgctggcggc ttatgaagca
     4501 gggcaggcac cgaagccgaa tattcatggc aatactcgct gccagaatat tgtgcgctac
     4561 gaaatgttca aaaagctggg ctacttcgtc acggaatcgt cagaacattt tgctgagtac
     4621 acaccgtggt ttattaagcc aggtcgtgag gatttgattg agcgttataa agtaccgctg
     4681 gatgagtacc cgaaacgctg cgtcgagcag ctggcgaact ggcataaaga gctggaggag
     4741 tataaaaacg cctcccggat tgatattaaa ccgtcacggg aatatgccag cacaatcatg
     4801 aacgctatct ggactggcga gccgagtgtg atttacggca acgtccgtaa cgatggtttg
     4861 attgataacc tgccacaagg atgttgcgtg gaagtagcct gtctggttga tgctaatggc
     4921 attcagccga ccaaagtcgg tacgctacct tcgcatctgg ccgccctgat gcaaaccaac
     4981 atcaacgtac agacgctgct gaccgaagct attcttacgg aaaatcgcga ccgtgtttac
     5041 cacgccgcga tgatggaccc gcatactgct gccgtgctgg gcattgatga aatatatgct
     5101 cttgttgacg acctgattgc cgcccacggc gactggctgc caggctggtt gcaccgttaa
     5161 aacgcgacta aacgctactg cgccggggga tttattccgg cgcacacctc tgacgatacc
     5221 aataacagaa ggcgggcgtt ggtaacagcg acccgatacc ctatgagcat ttcaatgact
     5281 acaaaactca gttatggatt tggagcgttc gggaaggatt ttgcgatcgg cattgtgtat
     5341 atgtacctca tgtattacta caccgatgtc gttgggctgt ctgtgggttt ggtcggtact
     5401 ttgtttctgg tggcgaggat ctgggatgct attaacgatc cgattatggg atggattgta
     5461 aatgctacgc gatcgcgatg gggtaagttc aaaccctgga tcctgatcgg tacgttggca
     5521 aactctgtaa tcttatttct cctctttagt gcgcatctgt ttgaaggtac tactcagatt
     5581 gtctttgttt gcgtgaccta catcctctgg ggcatgactt acaccattat ggatattccc
     5641 ttctggtcgc tggttccaac catcacgctc gataaacgtg agcgcgaaca actggttcct
     5701 tatccgcgtt tttttgccag tctggcaggc tttgttacgg caggtgtgac gctaccattt
     5761 gttaattatg tcggcggtgg cgatcgggga tttggctttc agatgttcac tctggtacta
     5821 atcgcctttt ttattgtttc aaccatcatc actctgcgca atgtgcatga agtcttttcg
     5881 tcagacaatc aaccgtctgc tgaaggaagc catctgacac ttaaagccat cgttgcgcta
     5941 atttataaaa acgatcagct ttcatgcctc ttgggtatgg ctcttgctta taatgtagcc
     6001 agcaacatta ttaccggatt tgctatctat tatttctcat atgttatcgg tgatgcggat
     6061 ttgttcccct attatctgtc gtatgcggga gctgctaacc tggtgacgtt agtattcttc
     6121 ccacgcttag ttaaatcatt atcccgacgc attttatggg ccggagcatc tattcttccg
     6181 gtgttaagct gtggtgttct cctgttaatg gcattaatga gctatcacaa cgtcgtcctc
     6241 attgtgattg cgggtatttt gctgaatgtg ggaacggcgc ttttctgggt attacaggtc
     6301 atcatggtgg cagataccgt tgattacggt gaatataaac tgcacgtacg ctgtgaaagt
     6361 atcgcttact ccgtgcagac tatggtggtg aagggcggtt cagcctttgc ggcttttttc
     6421 attgcggttg tgttagggat gattggctat gtaccgaatg ttgaacagtc tacgcaagcc
     6481 ctattaggta tgcagtttat tatgattgct ctaccaactc tgtttttcat ggtaacgctg
     6541 attctctact tccgtttcta tcgcctcaat ggcgacacgc tgcgcaggat ccagattcat
     6601 ctgctggata aatatcgcaa agtaccgccc gagcctgttc atgctgatat tccggtcggt
     6661 gcagtgagtg atgtgaaagc ctgacgtgga caatagctaa cgaaaatggc ctgatgcgat
     6721 atgtttatca ggccacgtct taatgtaatg ctttggattt gcaaattttg tagaccggat
     6781 aaggaattca cgccgcatcc ggcatcaaca aagcgcacgt tgttatccgg ttatcaagcc
     6841 aaagcgccgt agctggcggc aatgaaagcg agcgaggaat atcaccgtcg ttaatgcgcc
     6901 aagcagcgcg cagaacatat cggattgcgt gtcccactgg tcgccctggg tgccgagaaa
     6961 atcatccgct ccctggccca ttgccagcgc cgcccaccac tcgattaatt catacatcgc
     7021 gcttatcgcc agcgccacgc agcagaccag gaacgccacc attttacgtc cgcgcacgta
     7081 catcccgcgc acgagaattt ctcgtgccac cagtgcaggc accagcccct ggaaaaaatg
     7141 ccccagcttg tcatacggat tacggctcaa ccccagccat tcctgcacct caaaaccgat
     7201 ggggactttc gcgtaggtgt attgcccgcc gaccatcagg atgatggcgt gaaagaaaat
     7261 gagcgtatag agcagcggcg ttaacggata acgtttagcg gtggcaagca gtagcggcac
     7321 gacaataatc accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc
     7381 ggtataaatc aggattaacg ttagcgccag tgcgccggtg ttaagaatta acggcttgag
     7441 tgtgcgggtc atggtgagtt cgcaagtcag ggaaaatact gactattcac cagcaagcgt
     7501 gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg
     7561 ctgggccgaa gaggttactt agtgcagttc gcgcactgtt tgttgacgat ctgctggaag
     7621 aagtcgttac ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc
     7681 cagatagctt ccatacccag ctccggataa gcgacgcatt ccagatgctt gatgctctgc
     7741 tgcgccagta ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatga
     7801 cacgcgtcgg taacctgctg actgcggtta cctttcgcca gcatgatcat gctgccgccg
     7861 tgggattgca gcagatccac gtaggagtcc atacggcctg cggtggttgg gccaagtgaa
     7921 cctgatggat aaccggcagg ggttttcgcc ggacccgcgt agtagatcgg gtgatctttg
     7981 atgtactgcg gaagttcttt accggcgtca atcagctctt tcagcttggc gtgtgcaata
     8041 tctcggccca cgataatggt gccggtgagc gacaaacgag tggataccgg gtattgcgaa
     8101 agctgggcga ggatctcttt catcgggcgg ttaaggtcaa ctttcaccgc ttcgccttca
     8161 ccggcctggc gcagttcttg tggaatgtac tggcctgggt tgtgttccag tttttcgatc
     8221 cagatacctt cgcggttgat tttcgcttta atgttacggt cagcggagca ggagacgccc
     8281 atgccgaccg ggcaggatgc gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg
     8341 aagtatttac cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc
     8401 tgttccagct ggacatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca
     8461 tcgtaatagt gagcgcttgc taacttgacg gttttcaggt tggtttccgc agacgtaccg
     8521 ccaatcacaa acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc
     8581 tcgacgagga agtttttcag tttgccggga gtcagcaggg ctttggtttc ctggtagaga
     8641 tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca
     8701 tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc cggtgttgac ctctttgtac
     8761 atgtccagcg ccgcattctg tgaatagcgc aggttatctt cgatataggt gttatagacg
     8821 cctttcgaca gcgcttcttc atcaccgccg ccggtccaca cgcgctggcc ttttttaccg
     8881 acgatgatcg cggtgccggt atcctggcag gtcggcagca cgcctttggc ggcgatttcg
     8941 gagtttctta agaattgcag cgccacgtac ttgtcgtttt cgctggcttc tggatcgtga
     9001 agaatagccg caacctgttt ctggtgtgcc ggacggagca tgaaagaagc gtcgtgaaag
     9061 gcttgctgcg ccagtagggt cagggcttct ggttccactt tcaggatggt ttcgccgtcg
     9121 aagtcggcaa cgctaacgta atcggaagtg agtagatagt attcggtatt gtctttcccc
     9181 atcgggaaag gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc
     9241 tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt
     9301 ataagaaccc gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctaacaccaa
     9361 tcaaccccgg cagaataaaa ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg
     9421 aacggtcaaa ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt
     9481 agcaagccgg tgctgaagcc acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg
     9541 gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa cttggaaacc agcagcagaa
     9601 caatggcata cgcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag
     9661 acatatgcgc accgaacatg gtttctgcca tccatgcgat accgtacacc gccacgatgg
     9721 cgatcatacc ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca
     9781 ggataataat cagcgccccg gtcagcagca taaacatctg aataaccagt accatcgaca
     9841 gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc accaagaagg gcgactacag
     9901 cgattgcccc gaggaaaatc cacattgcca gccagttgct tttcggcagt tttttatcga
     9961 gcagcgtcgc ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct
    10021 ggaactcttc gtctttatcc agatctttac cgcggaacca gctgaagata ccgatcgcca
    10081 ggataccgat taacgtcgat ggaatggtga ttgccagcag gtcgaggaac tcaagatggc
    10141 gaccatcaaa ggtgacatta cccagcattg caaccagaga cacgaccgca accgacaccg
    10201 gactggcgat aatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac
    10261 ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag aatggtgtaa accacatgac
    10321 ccgtaccgca aagaatggtc agtgtacagg tcacaaacgg cgcgacaatt gagacatatt
    10381 tcgggttgcg gcgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag
    10441 cttgcaaggt cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaaccggtg
    10501 gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata
    10561 aacccagcgc gataccaccc tttctggcac cataaaacag acatatcagt attatgataa
    10621 gttggatagt aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa
    10681 tagatcacag tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt
    10741 ttggtcgtta ccggctttag caaatacctc acagtaaata ttggctgatt aaagcagcgg
    10801 caaatttatg cactgtgata agcggctttt tcagagagaa aaactctctg gcgatgggtt
    10861 tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc
    10921 ggtctatttt ttgttctcag ggcagatatc attaggttta aagattaatc tgatctaccc
    10981 atttgtgggt aaaaatacac ataatgcggg tgacataata gttaattaac ttttgttatc
    11041 gttttgaaat taaaaacacc gttcacctga agagatatta atttttagcg atgatggagg
    11101 gataattata tttgatctgg cacaagtttt actgatgaag gatgtaactt gtgccagggg
    11161 tattttgcat tacggtaata attattactt acagataacc agcgatccgt tattggcaat
    11221 attgtttcag tagtgagtag tgttctgcct gaatacggta acggtaaacc ggacgccccg
    11281 tgacaccata atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt
    11341 tacggcagga aacacgcgag atgttaacct cgttagccag ctcgtcggtt gaaaactcat
    11401 agtcctgatg cgcgtcaatc cactggcaca gcgtgcgtaa cgtctgcggc gttaagcctt
    11461 ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct
    11521 cggcctggtc ataatactga tgtttttcca gcgccatttt cttttgccgc cagccggtga
    11581 gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat
    11641 gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt
    11701 tgcaacgcgc gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga
    11761 tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct
    11821 ccagcgtcga ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat
    11881 tcagctccgc gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct
    11941 ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac
    12001 ggcgatgctg ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg
    12061 ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat
    12121 ccccggtcca tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac
    12181 gctaatttcg ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc
    12241 caatgtggta atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gctggctttc
    12301 actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcgg ttaatcttgc tgattaaaaa
    12361 accagcgata accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt
    12421 attggctgtt ttgagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc
    12481 gagaatcaca tgcaatttat tcataaattc gtgggatcgt tcacgaagtg cgtcagcata
    12541 gttgaccaga ccatcgagtc gctgcatcag tttacgtact tcagttttgt ccctgaaggt
    12601 tgaaatggca ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag
    12661 ccgatcttta atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga
    12721 gacatctacc acctgtgacc atgagtggct gagcgtcgac agtttctcat cgtcctgcga
    12781 cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc
    12841 gtccacggca acgacgcctt ctttgataga ctgcaacatg gcctggcgct gctcaaacag
    12901 cgtggagatt tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat
    12961 gcaggtgcca atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg
    13021 actgtcattg atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat
    13081 ttgtttatga ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc
    13141 gcgattgata gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa
    13201 tggctgacca atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac
    13261 gacaataaac agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggattccgct
    13321 ctcctgcggt tttttctgca agccctgacg gatttccggc gagtcggcga gggtacgcgc
    13381 cactgccagt gccttgttgg ctagcccgtc tcgcgtcata tcactgattt gcgagaagta
    13441 aatcagatgc accaccaata gcaccgagaa cagtaccgca ctgaccatta agatcactgt
    13501 ggtactcaat ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag
    13561 cttccttgtg tgacaaattt cttaagcatt atctctgatg aggcgggtaa ttcaaaggga
    13621 gtaagaatga ttggctatat aggggaagag actctggcaa cggaaactgc cagtgctgta
    13681 tgaagattcc agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg
    13741 gatcaggcgc tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat
    13801 ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt
    13861 aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgtcac tgtggttaaa
    13921 gtgattgata cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa
    13981 tggaaatacg cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg
    14041 ctgaaattgt ctttgtgccg accggagaga atttagcgat tatcgaacat accgatgtcg
    14101 atgaaagcct gaaagggcaa gggattggta aacagctggt tgcgaaagtc gtggaaaaaa
    14161 tgcgtcggga aaaacgaaaa attatcccat tatgtccatt tgcgaaacac gaatttgata
    14221 aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt
    14281 cttgctctcc gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat
    14341 gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata
    14401 tctcctatat gagaatcatc aatcggggtt aataagtttt gcgtccccag agcattcaat
    14461 attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt
    14521 gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg
    14581 aatttctcct ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaactcc
    14641 ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc
    14701 ttagacttac cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt
    14761 cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact attctctaaa
    14821 atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg gtttatctca
    14881 catgagaaaa cacatattcg tttacttagt gcattcctgg tcggaataac ctggccaatg
    14941 agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag tggacccgct
    15001 ggttatcgtg aaacgaagcg tctctcctca aaactggatt tgcggaacga cgaacgaagc
    15061 agtcgcctga ctgcttcgtt cattaaagtg aaatttattt ctgtgggcgc atcgccggga
    15121 agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt cggtcgatac
    15181 caatacccag accagcggtt ggcggcagac catattccag cgcagtcacg taatcttcgt
    15241 catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc tggaaacgtt
    15301 cagcctgatc ttctacgtcg tttaattcgc taaaaccatt accgatttca cgaccaccga
    15361 tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc gccagcggtg
    15421 acacttctgc cggatattcc gtaataaagg ttggctgaat cagatgtgct tctgccactt
    15481 catcaaagat ctccgtgaca atacgtccca gcccccagct tttctctacc gtaattccga
    15541 tagattcagc taatgcttta gcagcatcaa aattatccag gtcggccata tcggtttctg
    15601 gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa
    15661 acacatgctc gccataagtg actttagtgg tacccagaac ctcttgtgcc agggtgcgga
    15721 acagggactc tgtcagttca atcaaatcgt ggtaatccgc atacgccatg tagagttcca
    15781 tcattgtgaa ctcaggatta tggcgaacag aaataccttc attacggaag ttacggttga
    15841 tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc tccggcgcga
    15901 tacgcaggta catatctaaa tccagagcgt tatgatgggt aataaacggg cgagcagatg
    15961 ccccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg cgcgcgacca
    16021 tgaactggcg gatagcggcc agaatttttg aacggacaac aaacgtttga cgggatttat
    16081 cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc tgcagaccat
    16141 ggaatttatc tggtaaagga cgtagtgctt ttgtcagcag gcgcaactca gtacagtgaa
    16201 tggaaagctc accggtttgc gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca
    16261 gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct cttgcaacgt
    16321 acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc cccatgatac
    16381 gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc agttcctggt
    16441 tatccttcgc atcaaactct tcgtgcagct ggtcagaggt atggtcgcgg cgaaaatcat
    16501 tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt
    16561 catcgttaaa atcaatagcc tcattggctc cccgtgtttc ttgttcagac attttggttc
    16621 ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca ctgtattatc
    16681 tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc tcaagttgat
    16741 atcactaata aataaccatc aaaatggttt aaatgac
//
LOCUS       DAZFYC010000109        16372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFYC010000109 DAZFYC010000000
VERSION     DAZFYC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.109"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..1722)
                     /gene="pgi"
                     /locus_tag="ACHKW5_003888"
     CDS             complement(73..1722)
                     /gene="pgi"
                     /locus_tag="ACHKW5_003888"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO7513183.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      1959..2155
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2247..3596
                     /gene="lysC"
                     /locus_tag="ACHKW5_003889"
     CDS             2247..3596
                     /gene="lysC"
                     /locus_tag="ACHKW5_003889"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO7513184.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3741..4667
                     /locus_tag="ACHKW5_003890"
     CDS             3741..4667
                     /locus_tag="ACHKW5_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO7513185.1"
                     /translation="MATLMEKDALLNGASQCITFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4703..5494)
                     /locus_tag="ACHKW5_003891"
     CDS             complement(4703..5494)
                     /locus_tag="ACHKW5_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO7513186.1"
                     /translation="MPLNGVSAAMRERVSQQLREIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5500..6627)
                     /locus_tag="ACHKW5_003892"
     CDS             complement(5500..6627)
                     /locus_tag="ACHKW5_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO7513187.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHILPWFTTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVERYCVVADLSETEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            7156..7428
                     /locus_tag="ACHKW5_003893"
     CDS             7156..7428
                     /locus_tag="ACHKW5_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO7513188.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDHARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAAAKKARQLKKKQAYKPDTEASFNWSANTPTHGRR"
     gene            complement(7429..8301)
                     /gene="rluF"
                     /locus_tag="ACHKW5_003894"
     CDS             complement(7429..8301)
                     /gene="rluF"
                     /locus_tag="ACHKW5_003894"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO7513189.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPLVKMEKTSEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            8513..9202
                     /gene="pepE"
                     /locus_tag="ACHKW5_003895"
     CDS             8513..9202
                     /gene="pepE"
                     /locus_tag="ACHKW5_003895"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO7513190.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(9293..10924)
                     /locus_tag="ACHKW5_003896"
     CDS             complement(9293..10924)
                     /locus_tag="ACHKW5_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO7513191.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(11144..14827)
                     /gene="metH"
                     /locus_tag="ACHKW5_003897"
     CDS             complement(11144..14827)
                     /gene="metH"
                     /locus_tag="ACHKW5_003897"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO7513192.1"
                     /translation="MSSKVEQLRAQLNQRILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            15027..15851
                     /gene="iclR"
                     /locus_tag="ACHKW5_003898"
     CDS             15027..15851
                     /gene="iclR"
                     /locus_tag="ACHKW5_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO7513193.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDSVTEFGAMVIKAAKEVTLAYGGMR"
     gene            16168..>16372
                     /locus_tag="ACHKW5_003899"
     CDS             16168..>16372
                     /locus_tag="ACHKW5_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513194.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDF"
BASE COUNT         3967 a         4348 c         4029 g         4028 t
ORIGIN      
        1 atcacagaca aggggattta tctgataaaa caatgccgac ataacgtcgg cattgttatt
       61 aaggttttta aattaaccgc gccacgcttt atagcggtta atcagaccat tggtcgagct
      121 atcgtggctg ctgatttctt tatcatcttt cagctctggc agaatacggt ttgccagttg
      181 tttacccagc tccacgcccc actggtcgaa ggtgaagatg ttcaggatca cgccctgagt
      241 aaagattttg tgctcataca gcgcaatcaa cgcacccagg ctgaacggag tgatttcacg
      301 cagcaggatg gagttggtcg ggcggttacc ttcgaacact ttgaacggca ccacgtagtc
      361 aagcgttgcc ggatctttac cctgatcacg atattcctgc tcaaccactt cgcgggattt
      421 accaaacgcc agcgcttcgg tctgggcgaa gaagttagac agcagtttct ggtgatgatc
      481 agagagcggg ttatgggtga tagccggagc gatgaaatcg cacggtacca ttttggttcc
      541 ctggtggatc agctggtaga acgcgtgctg accgttagtg cctggttcac cccagataat
      601 cgggccagtc tggtaatcca caacgttacc gttacggtca acatacttac cgttggactc
      661 catattgccc tgctggaagt acgccgcgaa acggtgcata tactggtcat acggcagaat
      721 cgcctcagtt tccgcaccaa agaaattgtt gtaccagatg ccaatcagcg ccagcagtac
      781 aggcaggttt ttctcggcag gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga
      841 aagcagttca acgaagttat caaagccgat ggagagaaca atcgacaggc caatcgctga
      901 ccacaaagag taacggccgc cgacccagtc ccagaactcg aacatgttgg cagtatcaat
      961 accaaactcg ccaacggctt tggcattggt tgagagcgcc gcaaagtgtt tcgcaacgtg
     1021 tttttcatca cctgccgctt tcaggaacca gtcacgcgcg ctatgggcgt tggtcatggt
     1081 ttcctgagtg gtgaaggttt tagatgctac caggaacagc gtggtttccg ggtttacttt
     1141 tttcagcact tccgcgatgt gagtcccatc gacgttagaa acaaagtgca tgttcaggtg
     1201 atttttgtac ggacgcagtg cttcggtcac catgtacggg ccgaggtcag aaccgccgat
     1261 cccgatgttc actacgtcag tgattgcttt gccggtataa cccttccact caccggagat
     1321 aatcgcttcc gagaaggttt tcatcttctc cagcaccgcg ttgacttccg gcattacgtc
     1381 tttgccatca accaaaatcg gggtattgct acggttacgc agcgctacgt gcagcacagc
     1441 gcggttttca gtgcggttga tcttctcgcc agagaacatc gacttaatcg cgcccgccag
     1501 atcgcactct ttcgccagat cttgtaattt cgccagcgtc tcttcagtga tgcggttttt
     1561 ggagtaatcc accagcatct gatcgccgaa ggttgcggag aacttagaaa aacgatcgcc
     1621 gtctttagca aaaagatcgg cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag
     1681 tgcctgccag gcagcggtct gcgttggatt gatgtttttc attagcaata ctcttctgat
     1741 tttgagaatt gtgactttgg aagattgtag cgccagtcac agaaaaatgt gatggtttta
     1801 gtgccgttag cgtaatgttg agtgtaaacc cttagcgcag tgaagcattt attagctgaa
     1861 ctactgaccg ccaggagtgg atgaaaaatc cgcatgaccc cgtcgttgac aaccgccccg
     1921 ctcacccttt atttataaat gtactacctg cgctagcgca ggccagaaga ggcgcgttgc
     1981 ccaagtaacg gtgttggagg agccagtcct gtgataacac ctgagggggt gcatcgccga
     2041 ggtgattgaa cggctggcca cgttcatcat cggctacagg ggctgaatcc cctgggttgt
     2101 caccagaagc gttcgcagtc gggcgtttcg caagtggtgg agcacttctg ggtgaaaata
     2161 gtagcgaagt atcgctctgc gcccacccgt cttccgctct tcccttgtgc caaggctgaa
     2221 aatggatccc ctgacacgag gtagttatgt ctgaaattgt tgtctccaaa tttggcggta
     2281 ccagcgtagc tgattttgac gccatgaacc gcagcgctga tattgtgctt tctgatgcca
     2341 acgtgcgttt agtagtcctc tcggcttctg ctggtatcac taatctgctg gtcgctttag
     2401 cggaagggct ggaacctggc gagcgattcg aaaaactcga cgctattcgc aacatccagt
     2461 ttgccattct ggaacgtttg cgttacccga acgttatccg tgaagagatt gaacgtctgc
     2521 tggagaacat tactgttctg gcagaagcgg cggcgctggc aacgtctccg gcgctgacag
     2581 atgagctggt cagccacggc gagctgatgt cgaccctgct gtttgtcgaa atcctgcgcg
     2641 aacgcgatgt tcaggcacag tggtttgatg tacgtaaagt gatgcgtacc aacgaccgat
     2701 ttggtcgtgc agagccagat gtagccgcgc tggcggaact ggccgcgctg cagctgctcc
     2761 cacgcctcaa tgacggctta gtgatcaccc agggatttat cggtagcgaa aataaaggtc
     2821 gtacaacgac gcttggccgt ggaggcagcg attatacggc agccttgctg gcggaggctt
     2881 tacacgcatc tcgtgttgat atctggaccg acgtcccggg catctacacc accgatccac
     2941 gcgtagtttc cgcagcaaaa cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg
     3001 caacttttgg tgcaaaagta ctgcatccgg caacgttgct acccgcagta cgcagcgata
     3061 tcccagtctt tgtcggctcc agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata
     3121 aaactgaaaa tccgccgctg ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca
     3181 ctttgcacag cctgaatatg ctgcattctc gcggcttcct cgcggaagtt ttcggcatcc
     3241 tcgcgcggca taatatttcg gtagacttaa tcaccacgtc agaagtgagc gtggcattaa
     3301 cccttgatac caccggttca acctccactg gcgatacgtt gctgacgcaa tctctgctga
     3361 tggagctttc cgcactgtgc cgggtggaag tagaagaagg tctggcgctg gtcgcgttga
     3421 ttggcaatga cctgtcaaaa gcctgcggcg ttggcaaaga ggtattcggc gtactggaac
     3481 cgttcaacat tcgcatgatt tgttacggcg catccagcca taacctgtgc ttcctggtgc
     3541 ccggcgaaga tgccgagcag gtggtgcaga agctgcattt taatttgttt gagtaatatt
     3601 tccctactgg cgtataacgc tcaccgattg ttatacgcca tcctcactga aaacatagat
     3661 taaataatat ctgaaaagca aaatcactcc tgtagaacag tattataatc accgaaaaat
     3721 atcatttaac aggagtgtta atggctactt taatggaaaa agatgcactt ttaaacggag
     3781 ccagtcagtg catcacgttt ttatcaaata tcattgataa ttgctcagtt tcgtcgcatc
     3841 aggattcagg cgatgctctc aagcgattag tcagctatcg cgattatctt tattctaccc
     3901 ccgctgaact tgttgatttt acacaaggta agattctcct tcagcaggta agaactcaat
     3961 atcagcatga attcaacaat actacgcatt cagaaaataa agcatctttt gattctattt
     4021 ggcagcgtct gacaaatcat gaagtcacac cgcaacagca tcccattggt tttgttttag
     4081 gtgggcaacc cggagcaggt aaatcatctc tgattgaatt agccaaaaga gagacaaaag
     4141 ataatataat gattattaac ggtgatgatt ttcgatttct acaccctgat tttaattata
     4201 tctaccaaaa ttatggggat gattttgtta cccatacagc gaaattttcc ggcgaaacgg
     4261 tcgaacgtgc aattgagaga gcgatagtca gtaaactcaa cattgttgtc gaaggaacat
     4321 ttcgtaatgc cgcgacgccg ttacaaacat taaaaaaact caaagatgca ggctaccaaa
     4381 cagaagtcat gataaaaaca acctcagcgg cattaagctg ggaaagcacc aatgaacggt
     4441 atagtaagga caaagaggcg ggaaatattg cccgaaaagt ggataaaaac caccatgata
     4501 tcgtcaccgg tttgctggca gaaaatgccc gcaaggtgtt tgcttcgaac ctgtcagata
     4561 agtttgcggt gtattcgcgt gagaagatga tatttagttc acaagcggca acgaatgacg
     4621 atattgcaac acttatacag aatgaaatca gcgggaatac acaataataa taagagctga
     4681 tggcgtgaaa taacgccggg ctttatgccc ggcgcattac cgtctggtac attaagctat
     4741 ccagttcctt aacgtcccct tctctgctat ccggtaacag cggtggaatt tccccacgtg
     4801 cctgttccgc acgaataaac gcatgtaaca atggacggcg cggaccatat tccgcttctc
     4861 ctgcgcgctg tttacgttcc agtaattcgt ctatttctgc acgtaacggc gcatcaagtt
     4921 cgcttcccgc cagtaactca gcaaagcgca ttggcggtac gcctttaccc gcttcgaccc
     4981 agcgcacggc cagcagcgga cgcagcacgt aaaagtattt tttcagccgc acttcgtcgc
     5041 cttgtaaata accgcgaaag tttttccgcg ccatcgaata ataatgccaa cgcgcacgca
     5101 gcggagaaaa ccacttcggc accattgcct taagcgccgt tatcgtttct tcatcttgtt
     5161 gatagaccac aggagaatcc agccattcta tcagcgtcgg attcgcacct ttcaataatc
     5221 ccagcgcctt gcgccactcc cagcctgaaa catctaactc gtcgtcaata ggcaactcaa
     5281 taacatcgcg cggcgcctct actcgcagat accattccag cgggtgaaca tacaaaaacc
     5341 gtacatcgta gtcgctgtcg ggcgaggcaa atccccagcc gcggctccct gattcacagg
     5401 cgtacagtac tttcacatca taccgccgtt caatttctct taactgctgc gatacccttt
     5461 ctcgcatcgc agcactgact ccatttaatg gcatgttact caccctttga tattaaccaa
     5521 cccatgcaga acctgatcca gaccgccaaa tacagagatg ttatcgatcc gttcagccac
     5581 tctctccagc gtttccagtt cttttaagcg taacgctacc gggttgtttt ccatgacttt
     5641 agcggtattg agtaacgaac gcgtcgctgc cgtttcttca cgtcggcgaa tcacattggc
     5701 ctgcgcggat ttctccgcct caaccagctg cgcaaggata tttttcatat cgccaggcag
     5761 gacgatatct ttcaccccta acgaggcaat ctccatacca aatggcaaca tgcgcgattt
     5821 cacctgttcg ctgacaacgt catcaatgac ctgtttatct tccagcaatt catccaacgt
     5881 acgcgtacct accgcttcac gcagggcaaa ttgtaattcc cgatacaaat gatcaatcgg
     5941 cttcgttaac tggctaaatg ccaataacac gtcgctgtag cgccagttag cggcgaggtt
     6001 aatccgcaga ttgaccttat ctttcgttaa gatttcttgc ccgctgactt ccagtacctg
     6061 caagcgggtg tcaaccactt cagcgtcaac cagatggtta attttccagt atgcggtcag
     6121 ccccggcggt aacagcgcct gcgtttcacc atcgattttc agcacgccta catgccaggc
     6181 cggaacctgc accgttagta ttgcatcgcg gcctttaact gcccctttgc gccgtggttg
     6241 caataccgca ttcatcacct ccgtttgtac ctggacttgc tgcgtattca tccgcaccag
     6301 tgtcaaatca tcctctacac gccagtacaa acggcgcgtt gatggcggca ggatctcctg
     6361 caaaataccg tccatataca acgccccggc ttcagtctcc gacaagtcgg ccaccacgca
     6421 atatctttcc acccaatcag gctgaaagcg gcgcaaataa tccgccagca cgtcgggcac
     6481 ttcgctacca tccagcgtaa tcagcaacac ttccggggta gtaaaccagg gtaaaatatg
     6541 ctcgcctgca tgtagcactt tgtagtaatc gccgtttctg gagagtaatg ccagctgatc
     6601 ttttcttacg cttattttct taatcattat ctattccttg cttaacagcc gtcgcggaag
     6661 cggacacgaa gcgacagggg aattcccttt tgcctgttac ccggcgtctt tatcggcgac
     6721 gttatcgggg atcgctgact gcgcaatccc tcatttttta tttaataagt tacaaccagt
     6781 attcatttca ggaatgaatg tgggaatcga acccataatt ggcgtcaaag gctgacggaa
     6841 cacgcccgga aaacagcctg accgttcctg gtgtgccgac ggggtttaac cccctttgaa
     6901 attcagtatc aacgcaaaag tgttattgcg agaagcgtgc cagaattgag aaaaattctt
     6961 aaccaaatga aaaataaaga gttagtgaat tacttttgtt tgtaggggga aaacttttcg
     7021 ctaaatgtgt atctgtattt atcgtcaatt atcaagaaag ataaatattt aagaagagaa
     7081 caaaggaata ttatcagttc tgcttgcaaa tgaattcccg tacactgaat ccatcggcta
     7141 actgagggca acatcatggc actcccgcgt attacccaaa aagagatgac agagcgcgag
     7201 caacgcgagc taaaaacgct gctggaccac gcgcggattg cgcatggtcg cgtactgacc
     7261 aactccgaaa ccaacagcat taaaaaagag tacatcgata agctgatggt tgagcgtgaa
     7321 gcagctgcaa aaaaagcccg ccagttgaag aaaaagcagg cttataaacc agatactgaa
     7381 gcatcgttta actggtcggc taatacacca acgcacggca ggcgctgatt aacgcccctt
     7441 ctttttacgc cccggcgatg tgaaacgttt accatttgac gccgggcggc cacctttttc
     7501 tgatgttttt tccatcttca ccaacgggcg tttgatgccc gccgttttcg gtttggcctt
     7561 cactttaggt tttgcctcgg aagaggaatt ttcgatgagc ttaaacaggt cgattaactc
     7621 atcgtcagtt aaatcgcgcc actcacccag cggaatgccg cttaagctga cgttcataat
     7681 gcgcgtacgt tccagctttt tcacttcata gccgaaatgc tcgcacatgc gccggatctg
     7741 acggttcagc ccctgcacca gggtaatgcg gaagacaaac ggcgcttctt ttttgacttt
     7801 gcactttttc gtcaccgtcc cgaggatcgg cacccccgcc cccataccac gaataaattc
     7861 atcggtaatc ggtttatcga ccgtcaccag atactctttc tcatgatcgt tacccgcacg
     7921 gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt
     7981 atccaggcgg ccaatcggga acacgcgttt gctgtggtta acgaaatcga caatgttatc
     8041 gcgctcgcca tcttcggtgg tgctgacaat accgacgggc ttgttcaggg cgataagtac
     8101 caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt actttcacaa cgtcgccggg
     8161 tttcacctga tcgccaatgg tggctcgctt gccattaagg aacacattgc cttgctcgat
     8221 atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg
     8281 gactgatgag tcgggcagca taaattctcc tgtaaaaaac ggaatatacc gtacctttgg
     8341 gttgataaaa aatagtcatg agcgaactat ttgttatttc cattggcttg aattcgttac
     8401 ctgctcaaat cactttccag aagcaatgtg cgacatcgtg agccatgtca gtaagtggtt
     8461 aaattcgcag tggtagcatg cctcccgtga tttcttacta accggagaag taatggaact
     8521 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct
     8581 aattgctgaa cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac
     8641 gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc
     8701 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg ctgagatcgt
     8761 gattgtcggc ggcgggaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
     8821 ggcaccaatt actgacgtgg ttaaacgtgg cgctctgtat attggctgga gcgcaggcgc
     8881 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg
     8941 tttcgatgcg ctaaatctgt tcccgttgca aatcaacccg cacttcacca acgcgctgcc
     9001 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
     9061 agaactgacg attattggtc taccggaagg taactggatc acagtgagta aaggtcacgc
     9121 tacgctgggt ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggtgccgct
     9181 agaagctggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
     9241 gtgcctgatg cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact
     9301 cgtcccgtcc ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca
     9361 gcgagttcag gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc
     9421 tctgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa
     9481 tgcgaaagcg atgcttacta cggcgcaaac gacgagcgct ggtgacatcg ccagagaaga
     9541 acaccgacat tgccagtttt aaattactga gcaattgctc atagagcgca tccagttcct
     9601 tcaagccatc aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctgc
     9661 ccatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg
     9721 cccagcggcg cgactcttcc tccgccaact cttcttttgg catccgcgcc agatacagct
     9781 taatggcggt gtagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc
     9841 gtggctcgcc gtgcatcact ttatttagcc cttccatcat ctgttccatg gcgtcgccaa
     9901 tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga
     9961 catccagatg tttgggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
    10021 tgcaaaaccg tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt
    10081 agaagacgtg gaaatagatc accagttccg cttttggcaa tgacaacttc cccattgtct
    10141 ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac
    10201 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
    10261 gaccggaacc gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc
    10321 ctgcggcggt cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg
    10381 cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca
    10441 cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca
    10501 gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
    10561 gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga
    10621 aggtgagaat acgcgccatt agcgccgtcc cgacatctgc acccagcaca atgaccagag
    10681 ccggtgcgag ggctaccaga tcctgagcga caaacgaggt caccagcatg gtggtggcat
    10741 tactgctctg taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt
    10801 cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc
    10861 gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta
    10921 acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga
    10981 ccggaaacac gctgactggc atctttactg tgccttttga ccttcgtttt tccggctaag
    11041 attattccag cctgattcca gtataagtgt ttcatattaa taaagagaca gggcactcat
    11101 tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc
    11161 ccagattcgg tgccagccag cgctcaactt cggtaacgct cataccttta cggcgggcat
    11221 aatcttcaac ctgatcgcgc tgaatttgtg ctacagcgta gtacttgctg tccgggtggc
    11281 tgaagtacca acccgaaacc gatgcaccgg gccacatggc gaaagattct gtgagtttca
    11341 tgccagtgtg tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg
    11401 ggcaggccgg atagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt
    11461 cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga
    11521 gatactccgc aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt
    11581 aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca
    11641 cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga
    11701 gacagtagtt agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga
    11761 tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg
    11821 ggaacaggcc caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca
    11881 tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa
    11941 tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat
    12001 tacgcagagt ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg
    12061 gcgtgtaagc ctgccagtca aaagcgaaat cgttatcgcg cgccgcttcc agcgtgaccg
    12121 gtggtgtgcg cggtttctta cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg
    12181 tacgagcgac aaaatcatca cgctgagtat cggaaagcag cgccgccacc acaccaacgg
    12241 tgcgcgatgc gttctgtaca tacaccgtcg ggccgctgta gttctgctca attttcaccg
    12301 ccgtgtgcgc ttttgaggtc gtcgcgccgc caatcaatag cgggatggtg aagccctgac
    12361 gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa
    12421 gtccaatcag atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca
    12481 taacgccgag atcgacaatt tcgtagttgt tacattgcag caccacgcca acgatatttt
    12541 taccgatgtc gtggacatcg cctttcacgg tggcgatcac catcttgccg ttggttttac
    12601 cctgctcttt gctggcttca ataaacggtt cgagatacgc cactgcctgt ttcatgacgc
    12661 gcgccgattt gaccacctgt ggcaggaaca ttttcccttc gccaaacaga tcgccgacca
    12721 cgttcatacc gtccatcagc gggccttcaa tcacttcaat cgggcgcgta gcctgctggc
    12781 gggcttcttc ggtatcctgc tcgataaact cggtaatgcc tttaaccagc gagtattcca
    12841 gacgtttatt cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg
    12901 ttttgctgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcgcgac
    12961 ggttaagaat cacatcttcc accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg
    13021 ccagttgccc ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga
    13081 acaccgcgtg aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag
    13141 atacaccgcc ggaaatcagc gcgtgcggca gttcgcgttt gatatcttca cacgcgccga
    13201 taaagtcctg cgcgtagttg ttgtgctctt caatgccggt tgcgaccgcg aagatgttcg
    13261 ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc
    13321 gacggcaaat ctcgattttc cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca
    13381 ttaccaccac tgccgcaccg taacgacgca agagcttcgc gtgatggata aaggcgtcga
    13441 cgccctcttt catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt
    13501 tttcaatgac atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt
    13561 caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat
    13621 ccatgttgat atcgataatc tgcgcaccgt tttccacctg ctggcgggcc acatccagtg
    13681 cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg
    13741 tgcgttcacc cacgttcaca aacaggctat cttcgccaat gttcagcggc tccaggccgg
    13801 acaaacggca ggctacgggg atttccggca gtttgcgcgg cgctaatcct tctactgcac
    13861 gactcatcgc tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa
    13921 aacccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact
    13981 caccaaaggc gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc
    14041 gtgacagctc ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa
    14101 aggtcagagc ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga
    14161 gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca
    14221 gcgcttcaaa ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa
    14281 cggtttcaat caggatcaga tccgcgccac cttccaccag cgctttggtg gactctcgat
    14341 aagccgccac cagcccgtca aaagtgatat tacgaaatgc cggatcattt acgtccggag
    14401 aaatagacgc cgtgcggttg gtcgggccga gaacaccggc aacgtagcgc ggtttctccg
    14461 gcgtgcgcgc ggtccactca tcagcacaag ctcgcgccag tttcgccgcc gcaaagttga
    14521 tttccgccga cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg
    14581 tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca
    14641 cttccggttt gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg
    14701 caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca
    14761 taccgccgtc cagcaccaga atacgttgat ttaactgcgc acgcagttgt tccactttgc
    14821 tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac
    14881 tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt
    14941 aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt
    15001 ccacgataca gaaaaaggag gctgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa
    15061 aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga
    15121 aattactgga gtggattgcc gaatccaatg gcagtgtggc actcacagag ctggcgcaac
    15181 aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt
    15241 tcgtgcgtca ggtcggcgaa ctgggacatt gggcaatcgg cgcacatgcc tttatggtcg
    15301 gcagcagctt tctccagagc cgtaatttgt tagcgattgt tcaccctatc ctgcgcaatt
    15361 taatggaaga gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag
    15421 cgattattat cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg
    15481 gtaaattgcc gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag
    15541 aacaggtgac gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg
    15601 tgtctcctgt acatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg
    15661 acgatgagga acatgcactg gggctacgtt gccttgcagc gtgtattttc gatgagcacc
    15721 gcgaaccgtt tgccgcaatt tcaatttccg gaccgatttc acgcattacc gatgacagcg
    15781 tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg
    15841 gaatgcgctg actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc
    15901 aatagaataa gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt
    15961 cacacacttt tattaactca gcattatttt taaacatcaa accacttaat cattaacaaa
    16021 atcgcaagat ctcattccac cgaaactttt acggttgcat tatgagattg agcgatttat
    16081 gatatttctt tagttgtaat catattaaat tttttcactg ttctgatagt taaaattcaa
    16141 gacatcaata aacaatgaga tatttaaatg attactcgta tccctcgtag ttctttctct
    16201 gcaaatatta ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat
    16261 aaagaactcg aggataaatt ttctggcaaa gagctggcga cgcctctatt aaaaagcttc
    16321 tcagagaact gtagacaaaa tggtcgtcat atctttagta acaaggattt tg
//
LOCUS       DAZFYC010000110        16356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFYC010000110 DAZFYC010000000
VERSION     DAZFYC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.110"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..956)
                     /gene="yghU"
                     /locus_tag="ACHKW5_003900"
     CDS             complement(90..956)
                     /gene="yghU"
                     /locus_tag="ACHKW5_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO7513195.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            1161..3020
                     /gene="gss"
                     /locus_tag="ACHKW5_003901"
     CDS             1161..3020
                     /gene="gss"
                     /locus_tag="ACHKW5_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO7513196.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            3312..4811
                     /gene="pitB"
                     /locus_tag="ACHKW5_003902"
     CDS             3312..4811
                     /gene="pitB"
                     /locus_tag="ACHKW5_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO7513197.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLTV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(4860..5552)
                     /gene="yghT"
                     /locus_tag="ACHKW5_003903"
     CDS             complement(4860..5552)
                     /gene="yghT"
                     /locus_tag="ACHKW5_003903"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7513198.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDVEK
                     AITDFMTARGYH"
     gene            5726..6439
                     /gene="yghS"
                     /locus_tag="ACHKW5_003904"
     CDS             5726..6439
                     /gene="yghS"
                     /locus_tag="ACHKW5_003904"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7513199.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            6471..7229
                     /locus_tag="ACHKW5_003905"
     CDS             6471..7229
                     /locus_tag="ACHKW5_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513200.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            7275..8624
                     /locus_tag="ACHKW5_003906"
     CDS             7275..8624
                     /locus_tag="ACHKW5_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO7513201.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            8624..9448
                     /locus_tag="ACHKW5_003907"
     CDS             8624..9448
                     /locus_tag="ACHKW5_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513202.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLIRKQTKGDELTTDERKQFKYMRARYRHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            9460..10020
                     /locus_tag="ACHKW5_003908"
     CDS             9460..10020
                     /locus_tag="ACHKW5_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7513203.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            10051..11121
                     /gene="lptF"
                     /locus_tag="ACHKW5_003909"
     CDS             10051..11121
                     /gene="lptF"
                     /locus_tag="ACHKW5_003909"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7513204.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALYVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            11118..12197
                     /gene="lptG"
                     /locus_tag="ACHKW5_003910"
     CDS             11118..12197
                     /gene="lptG"
                     /locus_tag="ACHKW5_003910"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7513205.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(12233..13405)
                     /locus_tag="ACHKW5_003911"
     CDS             complement(12233..13405)
                     /locus_tag="ACHKW5_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7513206.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(13405..13653)
                     /locus_tag="ACHKW5_003912"
     CDS             complement(13405..13653)
                     /locus_tag="ACHKW5_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7513207.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(13685..14599)
                     /locus_tag="ACHKW5_003913"
     CDS             complement(13685..14599)
                     /locus_tag="ACHKW5_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7513208.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(14596..16317)
                     /locus_tag="ACHKW5_003914"
     CDS             complement(14596..16317)
                     /locus_tag="ACHKW5_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO7513209.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKAL
                     PEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRC
                     VSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPF
                     GYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCY
                     GLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHG
                     IEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGY
                     LYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISD
                     EERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASL
                     HVQESLA"
BASE COUNT         3943 a         4054 c         4263 g         4096 t
ORIGIN      
        1 agcgtcgcat caggcattgt gcacgattgc cggatgcggc gtgaacgcct tatccggcct
       61 ggatggcttg ctgcgacgaa caccaaccct tacccctgac gcttatcttc cgtattcgtc
      121 tcgaaatcac tggcgtcatg gcgctcatgc aactgctcat ttagcggtcc gttggtgcgg
      181 ttaacaatac gcccacgttt caccgccgga cgttcgccta cttctttcgc ccagcgttgt
      241 acatgcttat aactgcccgc atcaagaaac tctgcggcat catacacgcc gcctaacacc
      301 acattgccaa accacggcca aatcgccata tccgcaatgg tgtactcatc gcccgcaata
      361 aacttatgct gcgccagttg cttatccagc acgtcgagca gacgtttggc ttccatggta
      421 aagcggttga tggcgtactc aattttcatc ggcgcgtaat ggtaaaagtg accaaaacca
      481 ccgccgagga acggtgccgc gccctgtaac cagaacagcc agttcatcgt ttcagtacgc
      541 tttgccaaat cctgcggcag gaagtagcca aatttctccg ccagataaag caggatcgaa
      601 ccagattcaa acacgcggat cggcggatta tgcgtatgat cgcgcagcgc cgggatcttc
      661 gagtttgggt tcacttcgac aaagccgctg gagaattgat cgccatcgcc aatacgaatc
      721 agccaggcgt cgtactctgc accagtaacg cccagcgcca gcagctcctc aagcataatc
      781 gttactttct gaccgttcgg cgttcccagc gaataaagtt gcaatgggtg tttgccaacg
      841 ggcagcgttt tttcatgcgt cggaccagaa accgggcgat tgatattggc gaacgcgccg
      901 ccagcggatt tatcccacgt ccagactttc gcgggctgat aagtattgtc tgtcatagtg
      961 agttgccttc tgagtggtag tgttgaagca gtgtagcagg tcacttgctg tacagaatgt
     1021 gaatttttta accctttgcg ccagccgctg cgatgaggtg tatgattagt ctgacctctg
     1081 tccggttaat gtcgctgagc aggtaatcag agcacaacaa ttacgccaac tgcttaggtt
     1141 gtattgttaa aggtaaagtg atgagcaaag gaacgaccag ccaggatgcc ccgttcggga
     1201 cattattggg ctacgcccca ggtggggtag caatctactc ttcagattac agttctctcg
     1261 atccgcagga atacgaagat gacgccgtat tccgtagcta tatcgacgac gaatatatgg
     1321 gccacaagtg gcaatgcgtt gaatttgctc gccgttttct ctttctgaat tacggtgtgg
     1381 tctttactga cgtgggtatg gcgtgggaga ttttctcgct gcgcttcctg cgagaagtgg
     1441 ttaatgacaa catcctgcca ttgcaggcat ttcctaacgg ctcgccgcgt gcgccggtcg
     1501 cgggtgcgct tcttatctgg gataaaggcg gtgaatttaa agacactggc catgtcgcca
     1561 tcattaccca attgcatggc aacaaagtcc gtattgcgga acagaacgtg attcattccc
     1621 cgttgccgca agggcaacag tggacgcgtg agctggagat ggtggtcgaa aacggctgct
     1681 ataccctgaa agacactttt gatgacacca ccattctggg ctggatgatc cagacggaag
     1741 atactgaata cagcttaccg cagccggaaa ttgcaggcga gctgctgaaa atcagcggag
     1801 cgcgcctgga aaacaaaggc cagtttgacg gtaaatggct ggatgaaaaa gatccgctgc
     1861 aaaacgccta tgtgcaggcc aacggtcagg tgatcaatca ggatccttat cattactaca
     1921 ccattaccga gagtgccgag caggagctaa ttaaagccac caacgagctg cacctgatgt
     1981 atcttcacgc aaccgacaag gtgctgaaag atgacaacct gctggcgctg ttcgacatcc
     2041 cgaaaatcct ctggccacgt ttgcgtctct cctggcagcg tcgccgtcac catatgatca
     2101 ccggtcgtat ggatttctgc atggatgagc gtggtctgaa ggtttacgag tacaacgccg
     2161 actctgcctc atgtcatacc gaagcgggct tgatcctcga acgttgggcg gagcagggct
     2221 ataaaggcaa cggcttcaat ccggcggaag ggctgattaa cgaactggct ggtgcctgga
     2281 aacacagtcg tgcacgtccg tttgtccata tcatgcagga caaagatatc gaggaaaact
     2341 atcacgcgca gtttatggag caggcgctgc accaggcggg ctttgaaacg cgtatcttgc
     2401 gcgggctgga tgaactgggc tgggatgctg ccgggcaact gattgatggg gaagggcgac
     2461 tggttaactg cgtgtggaaa acctgggcgt gggaaaccgc gtttgatcag attcgtgaag
     2521 ttagcgaccg tgagtttgct gcggtgccaa tccgtaccgg tcatccgcaa aacgaagtgc
     2581 gtcttatcga cgtattgctg cgcccggaag tgctggtctt tgagccgctg tggacggtga
     2641 tccccggcaa caaagcgatt ctgccgatcc tctggtcgct gttcccgcac catcgttacc
     2701 tgctggatac cgatttcact gttaatgatg aactggtgaa aacaggttac gcagtgaaac
     2761 cgatcgccgg tcgctgtggc agcaatatcg acctcgtcag ccatcatgaa gaggtgctgg
     2821 acaaaaccag cggtaaattt gccgagcaga aaaacatcta tcagcaactg tggtgtttgc
     2881 cgaaagtgga cggtaaatac attcaggtat gtaccttcac cgttggcggc aactacggtg
     2941 ggacgtgttt gcgcggtgat gaatcactgg tcatcaaaaa agagagtgat attgaaccgt
     3001 taattgtggt gaaaaagtaa tacatgttgt agcaaccccg tactgattat gaagaataat
     3061 cagtacgggg attaatcaaa atattcaata tttatatatt ccaatattta ttcatttcat
     3121 atgtgaatat attaaccagt ggaatacctg tgttttgatt atttctaaag gttttgaatg
     3181 aatgcttatt gtctgataca caagaaataa cactcttttt atcgttaaaa aatgatattt
     3241 cactttgccc atgccgttaa agtcactgat aatgcgtccg ttcgtaaatt caaaatggcg
     3301 taatctaata tatgctaaat ttatttgttg gccttgatat atacacaggg cttttgttat
     3361 tgcttgctct ggcatttgtg ttgttctacg aagcaatcaa tggttttcat gacacggcga
     3421 atgcggtggc aaccgttatt tatactcgtg ccatgcaacc acaacttgct gtggtgatgg
     3481 cggcattttt taactttttt ggcgtgttat tgggcggact tagcgttgcc tatgccattg
     3541 tccatatgtt gccaaccgat ttgttgctga atatggggtc aacccacggc ctggcgatgg
     3601 tcttttccat gctgctggcg gcgattatct ggaacctggg aacgtggttc ttcggtttac
     3661 cggcctccag ttcgcacacc ttgattggtg cgattatcgg catcggttta accaacgcgc
     3721 tgttaaccgg ctcatcggtg atggatgcgt taaacctgcg tgaagtgacc aaaattttct
     3781 cctcgctgat tgtttcccct atcgtcggcc tggtcattgc gggaggcctg atattcctgc
     3841 tgcgacgcta ctggagcggg acgaaaaagc gtgaccgtat tcaccgcatt ccggaagatc
     3901 gcaaaaagaa aaaaggcaaa cgtaaaccgc cattctggac gcgtattgcg ctgattgttt
     3961 ccgctgcggg cgtggcgttt tcgcacggcg cgaacgacgg acagaaaggg atcggcctgg
     4021 taatgctggt actggtgggg attgcccctg ctggcttcgt cgtcaatatg aatgcgtccg
     4081 gctatgaaat tacccgtacc cgcgatgccg ttaccaactt cgaacactac ctgcaacagc
     4141 atcctgaact gccgcagaag ttgattgcga tggaacctcc attgcctgca gcatcgactg
     4201 atggcacgca agtaacagag tttcactgtc atccggcaaa tacctttgat gctattgcgc
     4261 gcgttaaaac gatgctgcca ggcaatatgg aaagttacga gccgttaacc gtgagtcagc
     4321 gcagccagct gcgccgcatt atgctgtgca tctctgatac ctccgcgaag ctggcgaaac
     4381 tgccaggcgt cagtaaagaa gaccagaacc tgctgaaaaa actgcgcagc gatatgttaa
     4441 gcaccattga gtacgctccg gtgtggatca tcatggcggt agcactggcg ctcggcattg
     4501 gcaccatgat tggctggcgt cgtgtagcga tgaccatcgg tgagaagatt ggtaagcgcg
     4561 gcatgacgta tgcgcaaggc atggcggcac aaatgacggc ggcagtgtct atcggccttg
     4621 ccagttatat tgggatgcct gtctccacaa cacacgtcct ctcgtctgcg gtagcaggga
     4681 cgatggtggt ggacggcggt gggttacagc gtaaaacggt aaccagcatc ctgatggcgt
     4741 gggtgtttac tttaccggcg gcaatttttc tttctggtgg gctgtactgg atagcattgc
     4801 agttgattta accttcctga aaatgcccgg tcctggcgat cgggcatttc catttttgac
     4861 tagtgataac cacgcgcggt cataaaatcc gtaatcgctt tttctacatc aaccaacacc
     4921 tgttccagtg gctgattggc atcgatatca accagttgtg caccaccaaa ggttaactgt
     4981 ggcgttatgg caatcttcct cgccagcgat tcccgtttat ggtcgggttt acgtgcacag
     5041 gcaacttcaa ggtcaacatt gagtttgatg accagatcag gcttatggct cgccatccag
     5101 tgaaacgctt tacgttcctg gcttgccagc catgagacaa aacgaccacc ttcaacgtta
     5161 ggtgggaaca ccgtaccatc gtaagcgcca ggaatttggt cctgaggata acggtcggtt
     5221 agaacaatta acccgcgacg acgacaggca agcatatgac gaaagcgcag taagcgacgg
     5281 gcgacaaacg ctgtaattac cagcgccgga actggtccag gcaatttttt tgctgttttc
     5341 acctgatttc gttcaattgt tttatgtaag gattttccca tcaacggtaa ttttgtcact
     5401 gcacgaccga catttccggc ctgttttcct aaatgaactc tttcggcagc accatatttt
     5461 tcgacaacgg taataagatg ttcacacacc gttgacttgc ctgaaccatc gctaccaata
     5521 acggcaatta atggaggtgt tattgactgc atgaatatat cctttaattt aaatatccat
     5581 taaaaatatt tatttggtta atatgttttt atgaaagcgt aattcaggtc aatgtcacaa
     5641 ttaaccattg tcacaataag gttgaacgga tattctgagc acggaccacg taatattcaa
     5701 tacattattt actgccgggt ttttcgtgaa tcaacgtaat atgtcaataa ttaattccac
     5761 gccagtgcgt gttattgcca ttgtaggatg tgatggttca ggtaaatcga ccctcacggc
     5821 aagcctggta aatgaactgg cagcaagaat gccaacagaa cacatttatc tcgggcaatc
     5881 gtccgggcga attggcgaat ggatttcaca gctccctgtt attggcgcac cttgtgggcg
     5941 ttatctgcga agtaaagcgg cacatgtgca cgaaaaaccc tcaacaccgc ctggcaatat
     6001 tactgcgctg gttatctatc tgctttcctg ctggcgggcg tacaagtttc gcaaaatgtt
     6061 gtgtaaaagc cagcaaggct ttctgctcat caccgaccgc tacccgcagg ttgaagtgcc
     6121 gggttttcgc tttgatggcc cgcaattggc aaaaaccacg ggcggtaacg gttggataaa
     6181 aatgttaagg cagcgcgagc tgaagctgta ccaatggatg gcatcttatt tgcccgtatt
     6241 gttgattcgt cttggcattg atgaacaaac cgcgtttgcg cgtaaacctg accaccaact
     6301 ggcagcgtta caggaaaaaa tcgccgttac gccgcaactg acattcaatg gcgcaaagat
     6361 ccttgaactg gatgggcgac accccgccga tgaaattctg caagcgtcac tacgcgcaat
     6421 tcacgccgcg ctttcctgat caccaaaatg gataggggat gatttaacga atggatgcac
     6481 tacaaactca aactgttaat agtacaaccg cgccgcagcc caactacatt ccggggctga
     6541 ttgcggtggt cgggtgtgat ggcaccggta aatccacact gaccaccgac ctggtgaaat
     6601 cgctgcaaca acactggcaa accgagcggc gctatctggg gctgctctcc ggcgaagacg
     6661 gcgacaaaat caaacgattg ccgttggttg gcgtctggct ggaacggcga ctggcggcca
     6721 aatcctcgaa aacccaaagc atgaaaacca aatctccggc gctatgggcg gcggtgatta
     6781 tgtactgctt ctcgctgcga agaatggcga atctacgcaa ggttcagcga ctggcgcaaa
     6841 gtggcgttct ggtggtcagc gatcgcttcc cgcaggctga aatttcgggc ttttattatg
     6901 atggaccggg gattggcgtc gaacgtgcga ccgggaaaat cagcatgttt ctggcgcagc
     6961 gcgaacggcg tttataccaa caaatggcgc aatatcgccc ggaattaatt attcgcctgg
     7021 gcattgatat tgagactgcc atctcccgca agcctgacca tgactatgcc gagctgcagg
     7081 acaaaatcgg cgtcatgtcg acgattggct ataacggcac aaaaattctt gagattgatt
     7141 ccagagcgcc ctacagcgaa gtgctggaac aggcacagaa ggcggtttct ctggttgcca
     7201 tcgtttctga ccgccgaagt ttaacttaag gctggcggga tactttttac tatcaacgat
     7261 aaggtcaggc tgaattggcg ggttttaaca tcaaacattg gtttgcagat ggcgcgtttc
     7321 gcaccattat tcgcaatagc gcctggttag gctccagtaa tgtcgtgagc gccttgctgg
     7381 gtctgttggc gctctcgtgt gccggtaaag ggatgacgcc cgccatgttt ggcgtactgg
     7441 tgattgtgca atcgtacgcc aagtcgatca gcgattttat taagtttcag acatggcaac
     7501 tggtggttca gtacggaacg ccagccttga ccaacaataa tccgcagcaa tttcgcaatg
     7561 tcgtctcatt ttccttctcg ctggatatcg tcagcggcgc ggtggcgatt gtcggtggca
     7621 ttgccttact gccattcctt tcccattcat taggtctgga tgaccaaagc ttttggctgg
     7681 cggcgctcta ttgcacgctc attccttcaa tggcttcctc cacgccgacc ggcattctgc
     7741 gtgcggtaga tcgcttcgat ttaattgctg tacagcaggc gacgaaacct tttctgcgcg
     7801 cagcggggag cgtcgtagcc tggtattttg actttggttt tgcgggtttt gttattgcct
     7861 ggtacgtgtc gaatctggtt ggcggcacca tgtactggtg gtttgcagcg cgcgaattac
     7921 gccgcagaaa tatccataat gccttcaaat tgaatctgtt tgagtctgcc cgacacatca
     7981 aaggcgcgtg gagttttgtc tggtcaacca acattgccca ctccatctgg tcggcgcgta
     8041 actcgtgcag cacagtgctg gtggggatcg tgttaggacc cgctgccgcc gggttattta
     8101 aaatcgccat gacattcttc gacgccgccg gaacgccagc gggtttgctg ggtaaaagtt
     8161 tttacccaga ggtgatgcgt ttagatccgc gcaccaccag accgtggttg ttaggtgtga
     8221 agtccggttt actggcgggc ggaatcggta ttctggtggc gctcgcggtg ttgatcgtcg
     8281 gcaagccgct catttcgctt gtgtttggcg ttaagtatct cgaagcgtat gacctgattc
     8341 aggtgatgct gggcgcaatt gtgatttcga tgctgggttt cccgcaggag tcattgctat
     8401 tgatggcggg aaaacagcgc gcttttctcg tggcgcaaac catcgcctca atcggctaca
     8461 tcgtattgct gttcatgttc tgtcatctgt ttggtgtgct gggagcagcg tttgcttact
     8521 tcggtgggca atgtctggat gtggtgctct cgttaattcc aactttaaag gcattttttc
     8581 agcgtcattc cttgctttat aacgcagccg gagagaaatc ctgatgatga gaaaatattt
     8641 cccgcttgag gcgagcgagc gtctatttgt tgcgattgag gaagatgatg tcgttgatgc
     8701 ccaggtcagt ttgccgccga ctattgcact aagttgtacc accgaaatca tccatgataa
     8761 ttacgcgtta tgcctgcagt tctggctcaa tggcgttaat cgtcaggagt tactgcgttt
     8821 aattcgcaaa cagaccaaag gcgatgagct gacgacagac gagcgcaaac agtttaaata
     8881 catgcgagcg cgctacaggc acctgcgttt tgcgcaacgg ctgtatttaa agaagcatca
     8941 ggccggattt ttattcggca aaacgaccgt ttttctgggg cgttttcagg acggctttcg
     9001 taacggtaag aaaaatattg tgtcgtatta cggcaacctg ttacggattt atttaagctc
     9061 tccggtctgg tcgttggtga actattcatt acgccatagc cagttagaaa gcgtaagcgg
     9121 ttttattgcc tatcggcaaa agcaaatgca taccctgaaa gagattattg ccaaaccgcg
     9181 actaaccggt agggaatttc atgatgtgcg aaaaatcatc agccagcagg tttcttatta
     9241 cgacacctta agatcgcttg atcctgaaaa taaagaggcg ttacagatat cccgcttcct
     9301 cgccgccatt aatggcctga tgggagataa acacgacgac atggtggcgg atgatatgga
     9361 aaaccgccag tcgtatgatg caccggtggc gctggatagc gatattcgcc agcgcttaga
     9421 gctgctgatt tcgcgttttc cgctataacc ggagcaatga tgtcatctcg tttgattatt
     9481 gcgctgatta tcatgctgtt agccccgggt gtgcaggcac acaatttcgt cactggcaaa
     9541 acggttaccc ctgtttatat ccaggaaggc ggcgagctgc ttctgaatag cgacgacgaa
     9601 attcactacc aaaagtggaa cagcacacaa ttggcgggaa aagtccgcat tatccagtat
     9661 atcgccgggc gtaagtcggc gaagaaaaag aactcactgt taattaaagc ggttgaagcg
     9721 gcaaactttc cgcaggatcg ttttcagccc accaccattg tgaacaccga tgatgccatc
     9781 tttggtactg gctattttgt tgttggcaaa atcgaaaaaa ataaacgccg ctatccgtgg
     9841 gcgcaattcg ttattgacgg caatggacag ggacgcgttg cctggcgctt gccggagcag
     9901 agttcaacca ttctggtgct gaataaagcc gggcaaattc agtgggcaaa ggacggcagc
     9961 ctgacgccgg aagaggtaga tcatgtcatt gcgttggcgc agaaactgat caatgaataa
    10021 aaaactcaaa ggattgaggg cgttagtgag atgaagctgg ttgaacacta catcatgcgt
    10081 ggtacgcgcc gtctggtgct gattatcgtc ggttttctga tctttatttt cgccagctac
    10141 tccgcacagc gttatctgac cgaagcggca aatgggacat tagctcttta tgttgtgctg
    10201 gatatcgttt tctacaaagt actgattgca ctggagatgt tgttacctgt tggtctgtat
    10261 gtgtcagttg gcgtgacgct agggcagatg tataccgact cggaaattac cgctatctct
    10321 gcggcggggg gcagtccggg acgcttgtac aaagccgttc tttatctggc gataccgcta
    10381 agtatttttg tcacccttct gtcgatgtat ggtcgtccgt gggcttatgc gcagatttat
    10441 caactggagc aacagtcgca gtcggagctg gatgttcgcc agttgcgggc aaagaaattt
    10501 aacactaacg ataacggacg aatgatcctt tcgcagacgg ttgatcagga taataatcgc
    10561 ctgactgacg cgctgattta tacttctact gccaatcgaa cccgtatttt ccgcgcccgt
    10621 tcggttgatg tggttgaccc atcacccgag aaaccgaccg ttatgttgca caacggcact
    10681 gcctatcttc tcgatcatca agggcgtgac gacaacgaac agatctaccg taatctgcaa
    10741 ttacatctga atccgctgga tcaaagcccg aacgtcaaac gcaaagcgaa atcggtcacg
    10801 gagctggcgc gctccgtctt tcctgccgat catgccgaac tgcaatggcg acaaagccgt
    10861 ggtctgacag cattgttgat ggcgctgctg gccatttcat taagtcgggt aaaaccgcgg
    10921 caagggcgat tttcaacgtt attgccactg acgttgctgt ttattgccat tttttatggc
    10981 ggcgacgtct gccgtacgct ggtggctaac ggcgcgattc ccctcattcc tggtttgtgg
    11041 ttagtgcccg gactcatgct aatgggcctg ctgatgctgg tcgcacgcga cttctctttg
    11101 ctgcagaaat tttcccgatg aatgttttca gtcgctattt aatccgtcat ctctttctcg
    11161 gttttgccgc cgccgcaggg ctattgctgc cgctttttac caccttcaac ttgattaacg
    11221 aactggatga tgtcagcccg ggcggttatc gctggactca ggcggtgctg gtggtgctaa
    11281 tgaccttacc gcgcacactg gtcgaacttt cgccgtttat cgcgttattg ggtggtattg
    11341 ttggactggg gcagttatcg aaaaacagtg agcttaccgc cattcgcagc acggggtttt
    11401 ctatcttccg tattgcactg gtggcgctgg ttgccgggat attgtggact gtttcgttag
    11461 gcgcgataga tgagtgggtg gcgtcgccat tgcagcaaca ggcgttgcaa atcaaatcga
    11521 ccgccaccgc gttgggggag gacgatgaca ttaccggcaa tatgctgtgg gccaggcgcg
    11581 gtaatgaatt tgtgacggtg aaatcgctga acgagcaggg ccagccggtg ggcgtggaga
    11641 tatttcatta tcgcgacgat ctctcgctcg aatcttacat ttatgcacgc agtgccacca
    11701 ttgaagacga caaaacgtgg atcctgcatg gtgtgaatca taaaaaatgg cttaacggta
    11761 aagaaacgct ggaaacatca gataatcttg cctggcaatc ggccttcacc agtatggatc
    11821 ttgatgagtt atcgatgccg gggaatactt tttctgtccg tcagcttaat cattacatcc
    11881 attatttgca ggaaaccggg caacccagca gcgaataccg ccttgcactg tgggaaaaac
    11941 tggggcaacc gatcctgacc ctggcgatga ttttgctggc tgtaccgttt acctttagcg
    12001 ccccgcgctc gccagggatg ggtagccgtc tcgctgtagg cgtcatcgtg ggcttactca
    12061 cctggatcag ctatcaaatc atggttaatc tgggattgct atttgcgtta agcgcacctg
    12121 ttaccgcgct cggtttaccg gtagcgtttg tgttggtggc gttgagcctg gtgtattggt
    12181 atgacagaca acattaaacc cgccgtggcg caaacaccac ggcgggctca cattactgct
    12241 tcagagtagc aaatgcctga atgatggcat cgatttgctc acgactgtgg gcagcattta
    12301 cgctacagcg cagcagcgtg atgcctgcag gcgcggcagg cggcaggata agattcacat
    12361 acacgcccag cgagataagc tcgcgccaga tgcgtaaacc ttcctctttc gaaccgatta
    12421 ttactggcac taccgggcta atatgtgaac ttaactcata acccagtttt tgcagaccgt
    12481 catacagatg gttggcgtta tccatcagct tttgccgcag ctctggttgt gtggcaatag
    12541 ttcttaacga cgagcgcacg gtcgcaatgc aggacggaga aggagaggcg gtaaaaatat
    12601 acgggcggct gccgtagcgc agcacttcca tcgcttccga accgacagca aaaccgccaa
    12661 tcgaggccag acttttactg aaagtaccaa cgataatatc cacatcatct tcaacgccga
    12721 cagcttcagc cagtccgcgc cccgttgcgc ctaacacgcc aaacgaatgc gcttcatcga
    12781 caatcaaata accaccgaga cgacgtttga tatcaacaat ttccgccagc ggcgcaacgt
    12841 caccgagcat gctgtagatg ccctcaacga tgataatcgc ctctttcgcc cgttcaccga
    12901 ggcgaaccat gcgccgttcc agatctttag catcgttatg gcggaaacga ataatttccg
    12961 cgccaccgag agaacaggcg tcataaatac tggcgtggct gtcggcatcc agtaacacca
    13021 cggcgttatg gtcggcaagt gcgctgatga cgcctaaatt agcggtataa ccggttgaaa
    13081 aaacgatagc ggtagggcga ttgaaaaact cggcaatctc tttctctaac gccaggtgcg
    13141 gcgcataact gccgtttgcc atacgagaac cggtggtgcc ggtgccttgg gccgctaacg
    13201 ctgcctgacc ttcgctgatg gcatcatgat taaaggttaa accgagataa ttattggtcc
    13261 cggccagaat cactttttta ttgccgatcc ggccttcggt ggcggaatac acttcatcaa
    13321 tacaggtgcc aaacggtgtt aacccgttgt cactaaattg cagtcgctcg ccagcaaggc
    13381 gcgcatattt atcgtatagc cccattattt attctccagc catggaagta gagtttccat
    13441 taattgcgca ggggttttga catccagcag aatattaata ggaatggaaa tatcgaatct
    13501 gtcttccagc atcattaaga gatccatcac tttgatcgac tccaggccaa gatcgttgac
    13561 gagatcgctg tcgggcttaa tttccacacc gttttcgacc aaatcctgta aacaggaaag
    13621 gatgtaatcc attactattt cacgatttac catagaaacg ttaacacacc tttaaacaag
    13681 acaatcaaaa tagcccgttg cgtaatgcgt gttccaggct aatcccggga aaccaattaa
    13741 tatcttcaga aatacggtta ttacttgccg accagtcggc gtgggttaat tcacgaattt
    13801 tcgagcgggt cagcataggt tctttacctg ccaggcgact caacgcggta ctgatatccg
    13861 caaggcaggt gagtaccggc agaggaatac caaccattcg cacggaaccg caacggacgt
    13921 tggcggcaag ttgctgtatg cgttgccagt catagccgcc agcgacgcca tcgcataatt
    13981 cataggtttg cgtctgtata gtttcggcgc ttaaccactg acccactgct tgcgcgaaat
    14041 cggtgacgtg cagaaaagag agctgtgtat ctggcgcacc aagtcgtggc agcaggccgc
    14101 gcagcatcca gtcaaacagc ggttttaact ctttatcgcc gggaccatat acggctgtcg
    14161 ggcgaaaaac gcccagcgta atttcgtcag ccattgcagt cagccgttgt tcggcgacgt
    14221 gtttggaatt tgcgtaccag gagagttcgg gatggcgcgc cgccagcgaa gagataaaca
    14281 gaaaacgttg gcaaaaaccg ctctcttttg ctgcttgcat caggcgcagg ctgccgtcaa
    14341 cgttacagcg ggtgaaaatc tcttctttgt gcccgcgcac ttgcccggcg caatggacca
    14401 ccgcgctggc tccggcaacc agcttgctaa gtgaatgagt atcttccagc gaaccgcgca
    14461 cccaggtgag attatcattg acgtgagcgc gggcagtacg cgtcaatgcg cgaacgtgaa
    14521 agccgcgggc gagcaggtta tcgataatat atttaccgat aaacccggta gcgcccgtca
    14581 ccgcgacagt ttgattcatg ccagggattc ctgcacatga aggcttgcag cataagcctt
    14641 ctgataacgt tttttcgctt ccgcacgggc aggcttgccg gaggatgttc ggggaatact
    14701 gtggggcggc aacagatcga tagccgctgt tacgccaaat tcgctttgga tccgtgctgc
    14761 cagcgcgtgg ataagctgcc cgcgacgttc ttcgtcgctg atccgacact ggatctgcaa
    14821 aatgattttt tcctgggcgg taacaaaagc aatcgcatcg ccagaatgaa tttccggttc
    14881 ttgttccgca atatattcaa tatcctgcgg ccagatatta cggccacgaa taataatcag
    14941 atctttaata cgtccggtga catacagata accgtccagc agataaccga ggtcgccggt
    15001 gtctaaccag cccgtcgcgg caatctcgtc ttgcgaaacc tggtcgccaa agtaaccgct
    15061 catcaggctg gggccggaga tgcaaatatg gcctaccaca cgttccgcga ccggcatacc
    15121 tgcttcattg cggatttcaa tgccatgttc cggcaacgct ttgccgcagt tgacgaaagt
    15181 cgatacggcg cgtgtttcag cacccggcgc gacggctttg ccctgatatt ctaggatgtc
    15241 gcgatccact tcgttaacca caaccccaga gccttcatca gagaagctga cagccagcgc
    15301 attttctgcc agtccgtagc acggcatgaa cgttttatcg tcaaagttaa cctgacggaa
    15361 acattcagcg aattgatgga gttgttctgc ggagatcggc tcagcaccaa taccagcgac
    15421 gcgccagcag gaaagatcca gttcagcgag atctttttca ttcacgcggc gctggcacaa
    15481 ttcatagcca aacggcggcg caacggaaac ggtgccgcga tttttactga tcaatttaag
    15541 ccattgcaga ggacgcatgg caaaatcctg agtgcgcaaa taatctactg aaagttgcgt
    15601 ggcgacgggg gtcaggagaa agccgaccag tcccatatca tggtagaaag gcagccagga
    15661 gacgcagcgg tcgccagggc gtaatttaat gccatcgtgg cttatagcac gtagattagc
    15721 catcacttcg cgatgggtga taatgacgcc acggggaaaa cgggtgctgc cggaggtgta
    15781 ctggaggtag gcgatatcgt tcggaactgg acgctggagc gcaacatcgg cttccggtaa
    15841 tgccttaaac caggcgtggc ttaaaacatg taattcgggg ttgttatgcg tcgcggcatt
    15901 gaccagtggc aaccactcat caccagtgat aatggctgcg ggctggcaac ttgccagtaa
    15961 accctggagt ttggcgctcc aggaatcccg ctgaccgacg cccattggaa tcgccaacgg
    16021 gacggcgact aagccagcat actgacaggc aaaaaaagcc tctacaaacc cgctactcgt
    16081 ttcggcaatc agcgccacgc gttcgccttt tttcaggttc agcgataaca accgcttcgc
    16141 accagcttcg gcacgcgttt ttaacgtctg atattccagt tgatcttcaa gttggcaacg
    16201 tctgtcataa aaattcattc cggcgctact cagagcggcg tagtccaaag catcaaccag
    16261 cgttggaaaa tcggcatagc gcataggtag ggaatgcgta aagattttat tagacatata
    16321 caccacgaaa aaattggata tcaaaaataa aatgac
//
LOCUS       DAZFYC010000111        16074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFYC010000111 DAZFYC010000000
VERSION     DAZFYC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.111"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..113)
                     /gene="yojO"
                     /locus_tag="ACHKW5_003915"
     CDS             complement(<1..113)
                     /gene="yojO"
                     /locus_tag="ACHKW5_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO7513210.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIA"
     gene            222..716
                     /gene="napF"
                     /locus_tag="ACHKW5_003916"
     CDS             222..716
                     /gene="napF"
                     /locus_tag="ACHKW5_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO7513211.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDWQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            706..969
                     /gene="napD"
                     /locus_tag="ACHKW5_003917"
     CDS             706..969
                     /gene="napD"
                     /locus_tag="ACHKW5_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO7513212.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            966..3452
                     /gene="napA"
                     /locus_tag="ACHKW5_003918"
     CDS             966..3452
                     /gene="napA"
                     /locus_tag="ACHKW5_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO7513213.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3459..4154
                     /gene="napG"
                     /locus_tag="ACHKW5_003919"
     CDS             3459..4154
                     /gene="napG"
                     /locus_tag="ACHKW5_003919"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO7513214.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4141..5004
                     /gene="napH"
                     /locus_tag="ACHKW5_003920"
     CDS             4141..5004
                     /gene="napH"
                     /locus_tag="ACHKW5_003920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO7513215.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5001..5450
                     /gene="napB"
                     /locus_tag="ACHKW5_003921"
     CDS             5001..5450
                     /gene="napB"
                     /locus_tag="ACHKW5_003921"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO7513216.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5460..6062
                     /gene="napC"
                     /locus_tag="ACHKW5_003922"
     CDS             5460..6062
                     /gene="napC"
                     /locus_tag="ACHKW5_003922"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO7513217.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6075..6698
                     /gene="ccmA"
                     /locus_tag="ACHKW5_003923"
     CDS             6075..6698
                     /gene="ccmA"
                     /locus_tag="ACHKW5_003923"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO7513218.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6695..7357
                     /gene="ccmB"
                     /locus_tag="ACHKW5_003924"
     CDS             6695..7357
                     /gene="ccmB"
                     /locus_tag="ACHKW5_003924"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO7513219.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7399..8136
                     /gene="ccmC"
                     /locus_tag="ACHKW5_003925"
     CDS             7399..8136
                     /gene="ccmC"
                     /locus_tag="ACHKW5_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO7513220.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8133..8342
                     /gene="ccmD"
                     /locus_tag="ACHKW5_003926"
     CDS             8133..8342
                     /gene="ccmD"
                     /locus_tag="ACHKW5_003926"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO7513221.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8339..8818
                     /gene="ccmE"
                     /locus_tag="ACHKW5_003927"
     CDS             8339..8818
                     /gene="ccmE"
                     /locus_tag="ACHKW5_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO7513222.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8815..10758
                     /gene="ccmF"
                     /locus_tag="ACHKW5_003928"
     CDS             8815..10758
                     /gene="ccmF"
                     /locus_tag="ACHKW5_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO7513223.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10755..11312
                     /gene="dsbE"
                     /locus_tag="ACHKW5_003929"
     CDS             10755..11312
                     /gene="dsbE"
                     /locus_tag="ACHKW5_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO7513224.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11309..12361
                     /gene="ccmH"
                     /locus_tag="ACHKW5_003930"
     CDS             11309..12361
                     /gene="ccmH"
                     /locus_tag="ACHKW5_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO7513225.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12396..13043)
                     /gene="narP"
                     /locus_tag="ACHKW5_003931"
     CDS             complement(12396..13043)
                     /gene="narP"
                     /locus_tag="ACHKW5_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO7513226.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13363..15954
                     /gene="yejO"
                     /locus_tag="ACHKW5_003932"
                     /pseudo
     CDS             13363..15954
                     /gene="yejO"
                     /locus_tag="ACHKW5_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3569 a         3940 c         4685 g         3880 t
ORIGIN      
        1 gctatataaa tatatttata accatttgaa atgtgagcaa aagcccgttt ttgccacgct
       61 tcccgccact cttttgatcc tgctacaggt tttaccccga tcggggtatg catctttgac
      121 acatccttta atatcttagc ggctataaaa atggcttatt aattatgcgg tttatttggt
      181 cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc
      241 gtcggggcat actcactggt cgctggcgca aagccagtaa cggtatccgt ccgccctggt
      301 ccggtgatga atctcatttt ctgacccatt gcacccgctg cgacgcctgt attaatgctt
      361 gtgaaaacaa cattctgcaa cgcggcgcgg gcggctatcc gagcgttaat ttcaaaaata
      421 acgagtgcag cttctgttac gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc
      481 gccacaccag ggcctgggat tggcagttca ccattgggga cgcgtgcctg gcgtatcagt
      541 ctgttgaatg ccgccgctgt caggatagct gtgagccaat ggcaattatc tttcgcccga
      601 cgctctccgg gatctaccag ccgcaactta atagccaact ttgcaacgga tgtggagcat
      661 gtgcagccag ctgtccggta tcagccataa ctgcggagta tctccatgca cactaactgg
      721 caagtttgca gcctggtcgt gcaggccaaa agcgaacgaa tttcagacat cagcacccaa
      781 ctgaacgcct ttcccggctg tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt
      841 gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac
      901 gtagagggcg tgctggcggt gtcgctggtt tatcaccagc aggaagagca aggtgaggaa
      961 acaccatgaa actcagtcgt cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg
     1021 cggctgccgg tctcagcgtg ccgggcgttg cccgcgccgt tgttggtcag caggaagcca
     1081 tcaaatggga taaagcgccg tgccgtttct gcggtactgg ttgcggcgtt ctggtcggaa
     1141 cgcagcaggg gcgtgtggtg gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc
     1201 tgaactgcat taagggctat ttcctgccca agatcatgta cggtaaagac cgtttgacgc
     1261 agccgctgct gcgtatgaaa aacggtaaat atgacaaaga aggcgaattt accccaatca
     1321 cctgggatca ggccttcgat gtgatggaag agaaattcaa aaccgccctg aaagaaaaag
     1381 gaccggaatc gatcggtatg ttcggttctg gtcagtggac tatctgggaa ggttatgccg
     1441 cgtccaagct gttcaaagcg ggcttccgtt cgaacaacat cgacccgaac gcacgtcact
     1501 gtatggcgtc ggcagtagtt ggctttatgc gtacctttgg tatggatgag ccgatgggct
     1561 gctatgacga catcgagcag gctgacgcgt ttgtgctgtg gggcgctaac atggcggaga
     1621 tgcacccgat cctctggtca cgcatcacta accgtcgtct ctctaaccag aacgtcaccg
     1681 tggcggtgct ttctacctac cagcatcgta gcttcgagct ggcggataac ggcatcatct
     1741 ttacgccgca atctgacctg gtgatcctga actacatcgc caactatatc attcaaaaca
     1801 atgcgataaa tcaggacttc ttcagcaagc acgttaacct gcgcaaaggg gcgacggaca
     1861 tcggctacgg tttacgtccg acccatccgc tggaaaaagc agcgaagaat ccaggttctg
     1921 acgcctccga accgatgagc tttgaagatt acaaagcctt cgttgccgag tatacgctgg
     1981 aaaaaactgc cgaaatgacc ggcgtgccga aagaccagtt agaacaactg gcgcagctgt
     2041 atgccgatcc gaacaagaaa gtcatctcct actggacgat gggcttcaac cagcatactc
     2101 gtggcgtgtg ggccaacaac ctggtctaca acctgcacct gctgaccggc aaaatttccc
     2161 agccgggttg cggtccgttc tccctgaccg ggcagccttc cgcgtgtggt actgcgcgtg
     2221 aagtgggcac ctttgctcac cgtctgcctg cggacatggt ggtgactaac gagaaacacc
     2281 gtgatatttg cgagaagaag tggaatatcc cgagcggcac cattccggcg aaaatcggtc
     2341 tgcacgcggt ggcacaagac cgtgcactga aagacggcaa gctgaatgtt tactggacca
     2401 tgtgtaccaa caacatgcag gccgggccga acattaatga agagcgtatg ccgggctggc
     2461 gcgatccgcg caacttcatc atcgtctccg atccgtatcc gacagtcagt gcgctggcag
     2521 ccgacttgat cctgccgacc gcaatgtggg tagagaaaga gggcgcttac ggtaacgccg
     2581 aacgccgtac tcagttctgg cgtcagcagg tacaggcgcc aggcgaagcg aaatcggatc
     2641 tctggcagtt agtccagttc tcccgccgct tcaaaactga agaagtatgg ccggaagagc
     2701 tgctggcgaa gaaaccggaa ctgcgtggca aaacgctgta cgaagttctg tatgccaccc
     2761 ccgaagtgag caaattcccg gtatccgaac tggcggaaga tcagctgaac gatgaatccc
     2821 gcgagctggg cttctatctg caaaaagggc tgttcgaaga gtacgcatgg tttggtcgcg
     2881 gtcacggtca cgatctcgcg ccgttcgatg actaccacaa agcgcgcggt ctgcgctggc
     2941 cggtggtgaa cggtaaagaa acacagtggc gttacagcga aggtaacgac ccgtacgtga
     3001 aagcgggcga aggctataag ttctacggta aaccggatgg caaagcggtg atcttcgcac
     3061 tgccgttcga accggcggcg gaagcaccgg atgaagagta cgacctgtgg ctctctaccg
     3121 gacgcgttct ggagcactgg cacaccggca gtatgactcg ccgtgtgccg gaactgcacc
     3181 gcgccttccc ggaagcggtc ttgtttattc acccgctgga tgcgaaagcg cgcgatctgc
     3241 gccgtggcga caaagtgaaa gtggtttctc gccgtggtga agtgatctcg attgttgaaa
     3301 cgcgcggtcg taaccgtccg ccacagggcc tggtgtacat gccgttcttc gacgccgcac
     3361 agctggttaa caaactgacg ctggatgcga ccgatccgct ctcgaaagag acggacttca
     3421 agaagtgcgc agtcaaactg gagaaggtgt aagccattat gtcccggtca gcgaaacctc
     3481 aaaatggtcg ccgccgcttt ctgcgcgatg tcgttcgcac agcaggcggg ctggctgccg
     3541 tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc
     3601 cgcccggagc cataaacgag aacgcctttg ccagtgcctg tgtgcgttgc ggtcagtgtg
     3661 ttcaggcttg cccttacgac accttaaaac tggcgacgct ggcctctggt ctgtcggcgg
     3721 gcacgccata ttttgtcgca cgggatattc cttgcgaaat gtgtgaggac attccgtgcg
     3781 ccaaagtgtg cccaagcggt gcgctggatc gtgagattga atcgatcgac gacgcgcgga
     3841 tggggctggc ggtactggtg gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg
     3901 atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat caccctggag ctggagcgca
     3961 acacgcgtac cggtaagcac gcccgctttc tgccgacggt tcacagcgac gcctgtaccg
     4021 gttgcggtaa gtgcgaaaaa gtatgcgtgc tggaacaacc ggcaatcaag gtgttaccgc
     4081 tgtcactggc gaaaggggag ttaggtcacc attaccgctt cggttggctg gaggggaaca
     4141 atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg agaaaaaagg ctggtggcgc
     4201 agtcaccgtt ggctggtgtt acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg
     4261 agcggtccat ggtttggtgt gtggatcttg cacggcaact acagcagtag cctgttattc
     4321 gacaccgttc cgctgaccga tccgctgatg acgctgcaaa gtcttgccag tggtcatctg
     4381 cctgccacgg tggcgttgac cggtgcggtg attatcaccg tgctctacgc cctggcggga
     4441 aagcgattat tttgcagctg ggtctgcccg ctgaacccga ttaccgactt agcgaactgg
     4501 ttacgcaggc gctttgacct caatcagtct gcgacgatcc ctcgccacat tcgctacgtg
     4561 ctgttggtgg tcattctgct gggatcggcc ttaactggca cgctcatctg ggaatggatc
     4621 aaccccgtat ctctgatggg gcgcagcctg gtaatgggct tcggcagcgg cgcgctgctt
     4681 attctcgcgc tgtttttatt tgatctactg gtcgttgaac acggctggtg cgggcacatt
     4741 tgtccggttg gcgcgctgta tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc
     4801 accgatcggc agaaatgtaa ccgctgtatg gattgttttc atgtttgccc ggaaccgcat
     4861 gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc
     4921 atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg tatttacaat aactacacga
     4981 tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga
     5041 cggcgatgct ggccctggtg gtaagcggcg cggtttgggc ggctaatggc gtcgatttta
     5101 gccagtcgcc ggaagtatcc gggacacagg aaggggccat tcggatgcca aaagagcagg
     5161 aacggatgcc gctgaactat gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt
     5221 atcaggtaac gaccaatacc aaccgctgct tgcagtgcca cggtgtcgaa agttatcgca
     5281 ccactggcgc gccgcgtatc agtcctactc actttatgga cagcgacggc aaagtgggcg
     5341 cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca cgtaccgcag gccgataccg
     5401 cgccaatcgt ggggaatacc tttaccccat caaaaggtta cgggaaataa gaggttatta
     5461 tgggaaattc tgaccgtaag cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc
     5521 ccagccgtct ggcgctgggg acgctgctgt tgatcggttt tgttggcggc atcgtcttct
     5581 ggggtggctt taacaccggg atggaaaaag ccaataccga agagttctgc attagctgcc
     5641 acgaaatgcg caacacggtg tatcaggaat acatggattc cgtgcactac aacaaccgta
     5701 gcggcgtccg tgcgacctgt ccggattgtc acgttccgca cgagtttgtg ccgaagatga
     5761 tacgcaagct caaagcaagt aaagagctgt atggtaaaat ttttggcgtt attgacacgc
     5821 cgcagaaatt tgaagctcat cgtctgacga tggcacagaa tgagtggcgg cgcatgaagg
     5881 acaataactc gcaggagtgc cgtaactgtc acaacttcga gtatatggat acaaccgccc
     5941 agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa agatgggcaa acctgtattg
     6001 attgccataa agggatagcg cacaagctgc ccgatatgcg tgaagtcgag ccaggttttt
     6061 aacagggtta ttgcgtgggt atgcttgaag ccagagagtt actttgtgag cgggatgaac
     6121 gaaccttatt tagtggcttg tcatttacgc tgaacgcagg agagtgggta caaatcaccg
     6181 gtagcaacgg cgcggggaag acaacgcttc tccgtttgct gacggggttg tctcgccctg
     6241 acgcaggcga ggttctctgg caagggcagc ccttgcatca ggtacgcgac agctaccatc
     6301 aaaacctgtt atggataggc catcagccgg ggatcaaaac ccggctgacg gcgttagaaa
     6361 atctgcactt ttatcatcgc gatggcgata ccgcacaatg tctggaagcc ctggcgcagg
     6421 ccgggcttgc cggattcgaa gatattcctg taaatcagct ctcggccggg caacaacgcc
     6481 gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt atggatcctc gacgagcctt
     6541 ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca gcgtatggcg cagcatacgg
     6601 agcagggggg gattgtgatt ctgactaccc accagccgct caacgttgct gaaagtaaaa
     6661 ttcgccgcat ttcactgacg caaacgaggg ccgcatgatg ttctggcgca ttttccgtct
     6721 tgagctgcgt gtagcgtttc gccatagcgc cgaaatcgcc aacccgctgt ggttcttcct
     6781 gattgtaatt accctttttc cgctcagtat cggtccggag ccgcaactgc tggcgcgtat
     6841 tgcaccgggc attatctggg ttgctgcgct gctttcatcc ttgctggcgc tggaacgact
     6901 gttccgtgac gatttgcagg acggcagtct tgaacaattg atgttgttgc cgttaccctt
     6961 gcccgccgtt gtgctggcga aggtgatggc gcactggatg gtaaccggtc tgccgttact
     7021 cattctttcg ccactggtag caatgctact gggaatggat gtttatggct ggcaagtgat
     7081 ggcgctgacg ctgctgctgg gaacgcctac gcttggcttt ctcggtgcac cgggcgtggc
     7141 gctgacagtg ggacttaagc gcggtggtgt gctgctcagc atactggtgt taccgctgac
     7201 tatcccatta ctcatcttcg ccaccgccgc gatggacgcg gcttccatgc atttgcccgt
     7261 tgacgggtat ctggcaattt taggcgcgtt gctggcaggc accgcgacat taagtccttt
     7321 tgcgacggcg gcagcgttac gaatcagcat tcaataacgc tggattactg atttttgttc
     7381 gagtctggta tcgaaactat gtggaaaaca ctgcatcaac tggcgatccc accacggctg
     7441 tatcaaatct gtggctggtt tataccgtgg ctggcaattg ccagtgtggt cgtgcttacc
     7501 gtcggctgga tctggggatt cggctttgct ccggctgatt atcagcaggg aaatagctac
     7561 cgcattatct acctgcatgt gcctgcggcg atctggtcga tgggcattta tgcatcaatg
     7621 gcagtggcag cgtttattgg ccttgtctgg cagatgaaaa tggccaacct ggcggtggcg
     7681 gcgatggccc ccattggtgc cgtgtttacc tttattgccc tggttaccgg ctctgcatgg
     7741 ggaaaaccga tgtggggcac ctggtgggta tgggatgcac gtctgacttc tgaactggtg
     7801 ctgctttttt tgtatgtggg tgtgattgcc ctgtggcacg ccttcgacga ccgccgtctg
     7861 gcgggccgtg cggcaggtat cctggtgctg attggcgtgg tgaatctgcc gattattcat
     7921 tactccgtgg agtggtggaa caccctgcat cagggatcaa cgcggatgca gcaaagtatc
     7981 gatccggcga tgcgttcgcc gctgcgctgg tcgatttttg gcttcctgct cctgtctgcc
     8041 acgctgacgc tgatgcggat gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg
     8101 gtgagtgaac tgatactgaa aagaggccgt aaatgacccc tgcatttgct tcctggaatg
     8161 aatttttcgc aatgggcggt tacgcctttt ttgtctggct ggcggtggtg atgaccgtta
     8221 ttccgctggt ggttttggtc gtgcactcgg tgatgcaaca tcgcgcaatt ctgcgtggtg
     8281 tggcgcaaca gcgggcgcgt gaggcgcgtt tacgtgctgc gcaacagcag gaggctgcat
     8341 gaatattcgc cgtaaaaacc gcttgtggat tgcctgtgcc gtgttggcag ggctggcgct
     8401 gactatcggt ctggtgctat atgcgctgcg ctcgaatatc gatctctttt atacgccggg
     8461 ggaaattctc tacggcaagc gtgaaactca gcaaatgccg gaagtcggtc agcgtctgcg
     8521 cgttggcggg atggtgatgc cgggtagtgt gcagcgcgat cccaattcgc tgaaagtgac
     8581 cttcactatt tacgatgctg aaggctcagt ggatgtctct tacgaaggca ttttgccgga
     8641 tctgttccgt gaagggcagg gcgttgtggt gcagggcgag ctggaaaaag gcaatcatat
     8701 cctcgcgaaa gaagtgctgg cgaaacacga cgaaaactac acgccgccag aagttgagaa
     8761 agcgatggaa gctaaccacc gtcgcccggc gagtgtttat aaggacccag catcatgatg
     8821 ccggaaattg gaaacgggct gctgtgcctg gcgttaggga ttgcgctgct gctgtccgtg
     8881 tatccgctat ggggcgtggc gcgcggagat gcgcgcatga tggcgtcttc ccgcttgttt
     8941 gcctggctgc tgtttatgtc tgtggctggc gcatttctgg tgctggtcaa tgctttcgtg
     9001 gtcaacgact ttaccgtcac ctatgttgcc agcaactcca atacccagct tccggtgtgg
     9061 tatcgcgtgg cggctacctg gggcgcgcat gaaggctcgc tcctgctgtg ggtgctgctg
     9121 atgagcggct ggacctttgc ggtggcgatt tttagtcagc gtattccgct ggatattgtg
     9181 gcccgcgtac tggcgataat ggggatggtc agcgtcggct ttttgctgtt cattctcttt
     9241 acctctaacc cattctcacg cacgctgccg aacttcccga ttgaagggcg cgatcttaac
     9301 ccgctgttac aggatccggg gctgatcttc catccgcctc tgctttatat ggggtacgtg
     9361 ggtttctcgg tggcgtttgc ttttgccatt gcttctttgt tgtgcgggcg tctggacagc
     9421 acttatgcgc gttttactcg tccgtggacg ctggcggcgt ggatcttctt gacgctcggc
     9481 attgtgctcg gttccgcatg ggcctattac gaactcggct ggggcggctg gtggttctgg
     9541 gatccggtag aaaacgcctc gtttatgccg tggctggtgg ggactgcgct gatgcactca
     9601 ctggcggtca ctgaacaacg cgccagcttc aaagcgtgga cattactgct ggcaatcagt
     9661 gccttctcgt tgtgtctgct ggggactttc ctggtgcgtt ccggcgtgct ggtatcggta
     9721 cacgcgtttg cctctgatcc ggcacgcggt atgtttatcc tcgcctttat ggtactggtg
     9781 attggtggtt cgctgctgct gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac
     9841 aatgcgctgt ggtcgcggga atctctgctg ttagcgaaca acgttttgct ggtcgccgcg
     9901 atgctggtgg tattactggg gacgctgctg ccgctggtgc acaagcaact gggactgggc
     9961 agtatttcga ttggcgaacc gttcttcaac accatgttta cctggctgat ggtgccgttt
    10021 gcgctgctgc ttggtgtcgg tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc
    10081 cgcaatttat tgattatcgc cttcatctca acgctggtgc tgtcgctgct gctgccgtgg
    10141 ctgttcgaaa gcaaagttgt ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt
    10201 gctgtgctgg caattgcgga agctgcgctg cgtatttcac gtggcacgaa aaccaccttc
    10261 agttattggg gaatggtggc ggctcacctg gggctggcag tgacaattgt tggtattgcc
    10321 tttagccaga actatagcgt tgagcgtgat gtgcgcatga agtccggcga tagcgtcgat
    10381 attcatgaat atcgcttcac cttccgtgat gtcaaagagg tgactggccc gaactggcgt
    10441 ggcggtgtgg cgactatcgg cgtaacgcgc gatggcaagc cggaaacggt gctgtatgcg
    10501 gaaaaacgtt attacaacac tgccgggtcg atgatgaccg aagcggcaat tgacggcggc
    10561 atcacgcgtg acctgtacgc cgcgctcggt gaagagctgg aaaacggcgc gtgggccgtg
    10621 cgtctttact acaaaccatt tgttcgctgg atttgggcgg gcgggctgat gatggcgttg
    10681 ggcggactgc tgtgtctgtt tgatcctcgc tatcgtaagc gcgtgagtcc gcaaaaaact
    10741 gcgccggagg ccgtatgaag cgcaaagtat tgttaattcc gttgattatc ttcctggcga
    10801 ttgccgcggc gctgctgtgg cagctggcgc gtaatgccga aggggatgat ccgaccaatc
    10861 tggaatcggc gctcattggc aagcctgtgc cgaagtttcg tctcgaatca ctggacaatc
    10921 cggggcagtt ttatcaggcg gatgtgctga ctcagggcaa accagtgctg cttaacgtct
    10981 gggcgacctg gtgtccgacc tgccgtgcgg aacatcaata tctgaatcag ctttcagcgc
    11041 agggcattcg cgtggtcggc atgaactata aagacgatcg ccagaaggca atcagctggc
    11101 tgaaagagct gggcaatcct tacgcgctaa gcctgtttga tggcgacggc atgttagggc
    11161 tggatctcgg tgtctatggc gcgccagaaa ccttccttat tgacggcaac ggtatcattc
    11221 gctatcgcca tgcgggcgat ctcaatcctc gcgtctggga agaagagatc aagccgctgt
    11281 gggagaaata cagtaaggag gccgcacaat gaggttttta ttgggcgtgc tgatgctgat
    11341 gatctccggc tcagcactgg cgaccatcga cgtgttgcag tttaaagatg aagcacagga
    11401 acaacagttc cgtcagctca ctgaagaact gcgctgcccg aaatgccaga acaacagcat
    11461 tgccgattcc aactcgatga ttgccaccga cctgcgtcag aaagtgtatg aactgatgca
    11521 ggaaggtaaa agtaaaaaag agattgtcga ttatatggtg gcgcgttacg gcaacttcgt
    11581 cacttacgat ccgccgttaa cgccgctgac cgtgctgctg tgggtgcttc cggtagtggc
    11641 tattggcatt ggcggttggg tcatatacgc ccgctcgcgg cgtcgggtac gcgtagtgcc
    11701 ggaagcgttt cctgaacaaa gcgtgcagga aggtaagcgt gccggatata ttgtttatct
    11761 gccgggtatt gtggtggcgt taattgtggc tggcgtcagc tactaccaga ctggcaatta
    11821 tcagcaggtg aaaatctggc agcaggccac ggcacaggct ccggcgttgc tggacagggc
    11881 gctggatccg aaagccgatc cgctcaacga agaagagatg tcgcgtcttg cgctggggat
    11941 gcgtactcaa ctgcaaaaaa atccgggaga tatagaaggc tggattatgt tgggccgcgt
    12001 tggcatggcg ctgggtaacg ctagtatcgc caccgatgca tacgctactg cgtatcgcct
    12061 cgatccgaaa aacagcgatg ccgcactggg ttatgctgaa gcgttgacac gttcatctga
    12121 tcccaacgac aaccgcctcg gtggtgaact gctgcgccag ttggtgagaa gcgaccacag
    12181 caatatccgt gtgttaagca tgtatgcgtt taatgccttt gagcagcagc gatttggcga
    12241 agccgttgcc gcgtgggaga tgatgttgaa actcttacct gccaacgata ctcgccgtgc
    12301 ggtgattgaa cgtagtatcg cgcaggcgat gcaacatttg tcgccgcagg agagtaaata
    12361 aaaagaggcc cgatggtgat gccatcgggc tatttttatt gtgccccgcg ttgttgcagg
    12421 aacagaatgg tggctgccac gcgtgagcgg acattgagtt tgcgcagcag attgcgaata
    12481 tgtactttta ctgtctgctc tgaaatattc aacaccgagg caatctgttt atttgacagc
    12541 ccctgtgcca gctcgtgcag aacatccagc tcgcgctccg tcagcacgct gaagggatct
    12601 tcttccgcgc caaacatttc acgttcacgt aagtactgat tgacgcgttc gctaaagact
    12661 ttgctgcctt tcgctccagc acgaattgct tccagcaata cttccgggtc gctgtctttc
    12721 aacagataac cgtctgcgcc tgcgtctatc agtgcaaaga catcgctgga ggcatctgat
    12781 acggtcagga taataatttg cgcggtaacg ccatccctgc gcaaggcatt gagggtatcc
    12841 aggccactca tacctttcat attgagatcc agcaagatca cgtcgatatc cagtctattc
    12901 gccagatcga tcgcgctcgc gccgtcgccc gcttcggcga ccacttcaaa gccaggatca
    12961 agctccagta actgacgaac accgcgtcgc ataagtggat gatcatccac aatcatcacc
    13021 tgaaaaggcg ttgcttcagg catagtagtc tcctgaggtt ttattagaat cattatgttt
    13081 gagcgtagcg ttggtaacgc taccaggcgc gtgaatgtta ccccatttta tgtatggaca
    13141 tgtagtgaaa actacaacct tgtcgttcgc tttggcatca ttttcattat aaagacggaa
    13201 ttttgcatca gcttctcatc aagaaaaaca actaaaaaat caatcacaat acttgtgcat
    13261 ttctttaaac agtttttaaa aactgactcc gggtatggag ctatgggtat tttccgtacc
    13321 caatgctttt aacagcaatt aatttcatag gatgaaagct caattcatca atctggttct
    13381 gtttctcttt gtcgttccgc aatatctgtt ctgatggcta cagcgttata ttcacccata
    13441 gcattggcat caacagttga gtatggtgag acagttgatg gtgttgtcct ggaaaaagat
    13501 atccagctgg tttatgggac cgccaataat acgaaaatca atcctggcgg agaacagcat
    13561 ataaaagaat ttggtgtaag tagtaatact gaaattaaag gagggtatca gtacattgaa
    13621 atgaatggta ccgcagaata ctcagtatta aatgacggtt atcaaattgt tcaaatgggt
    13681 ggcgcggcaa accagactac cctcaataat ggtgtgctac aggtttatgg cgcagcgaat
    13741 gatcccacga ttaaaggcgg gcgcttaatc gttgaaaaag atgggattac cgtccttgcc
    13801 gctatcgaaa agggaggatt actggaggtt aaagaggggg gattagcgat tgcggtagat
    13861 cagaaagcag gcggtgctat taaagcaagc acgcgggcca tggaggtatt cggaacaaac
    13921 cgtctcggtc agttcgatat caagaatggt attgctaata atatgttgtt ggaaaacggc
    13981 ggaagtttgc gagttgaaga aaatgacttc gcttataata ccactgtaga tagtggcggc
    14041 ttactggagg ttatggatgg cgggactgta actggcgttg ataaaaaagc aggcggaaaa
    14101 ttaattgtct caacgaatgc gcaggaagtg agtggtccaa acagtaaagg ccaatttagt
    14161 ataaaagatg gtgtgtcaaa aaattatgaa ctggatgatg gttccgggct cattgttatg
    14221 gaggacacgc aggccattga tactatcctt gatgagcatg ccactatgca atcgctggga
    14281 aaggatactg gtacgaaagt gcaggcaaat gcggtatatg atctcggtcg atcagatcag
    14341 aatggaagta tcacgtattc ctcaaaagcc atctctgaaa atatggttat caacaatggc
    14401 cgcgctaacg tctgggctgg cacaatggtt aacgtttcag tcagagggaa tgatggcatt
    14461 cttgaggtca tgaagccgca aataaattat gcacccgcaa tgttggtggg taaggtagtg
    14521 gtttctgagg gcgcttcttt tagaacgcat ggtgccgtgg ataccagcaa agcggacgtt
    14581 tcgctcgaaa atagcgtatg gaccatcatt gccgatatca ctacgacgaa ccaaaacacc
    14641 ctcctcaact tagccaacct tgcgatgtct gacgcaaatg tgattatgat ggatgagcca
    14701 gtgactcgtt catcagtgac ggcaagtgcg gaaaatttca ttacgttgac caccaatacc
    14761 ctgtcgggaa acggcaattt ttatatgcgt accgatatgg ctaatcatca gagcgatcag
    14821 ctcaacgtca ccggtcaggc aacaggtgat ttcaaaatat tcgtgacgga caccggtgcc
    14881 agcccggcag caggagatag ccttacactg gtaacaacgg gcggcggtga tgctgcattt
    14941 acgttgggca atgccggagg cgttgttgat atcggtacgt atgaatatac cttgctggat
    15001 aatggcaacc atagctggag tttggcagag aatcgcgcgc aaattacccc ttcaaccact
    15061 gatgtgctga atatggcggc agcacaaccg ctggtatttg atgcagaact ggacaccgtg
    15121 cgtgagcgtc ttggtagcgt aaaaggcgtt aattacgata cggcgatgtg gagttcggca
    15181 attaacaccc gcaacaacgt gaccactgat gcgggcgctg gctttgagca aacattgacc
    15241 gggctgacgc tcggtatcga tagccgtttc tcccgtgaag aaaccagcac aattcgcggc
    15301 ttgttctttg gttactctca ttctgatatt ggttttgatc gcggcggcaa aggcaatgtc
    15361 gatagctata ccctgggggc ttatgccggt tgggagcatc agaacggtgc ctatgttgat
    15421 ggagtggtga aagttgaccg ttttgccaac accatccatt gcaagatgag taatggggca
    15481 acagcgtttg gcgattacaa tagtaacggc gcgggtgctc atgtcgagag cgggttccgt
    15541 tgggttgacg gattgtggag tgttagaccc tatctggcct ttaccggctt taccacagat
    15601 ggtcaggact acacgttatc aaacggcatg cgcgcggatg tgggaaatac ccggatatta
    15661 cgcgctgaag cgggaacggc ggtaagctat cacatggacc tgcaaaacgg tacgacgctg
    15721 gaaccctgac tgaaagccgc cgtgcgtcag gaatacgccg attctaacca ggtgaaagtt
    15781 aatgacgatg gcaaatttaa taatgatgtg gctggaaccc gtggcgttta tcaggctggg
    15841 ataaggtcat cgtttacccc gacgttaagc ggtcatttgt cagtcagcta tggcaatggc
    15901 gcaggggtag aatcgctgtg gaatactcag gcgggtgtgg tctggacgtt ctgataacaa
    15961 aaaataaaca ggctgtgatg tgtcacggtc tgtttatcga attaattgca gatataaaaa
    16021 aaaccaaccg taaaggttgg ttttttcttg ggatttttgg tcggcacgag agga
//
LOCUS       DAZFYC010000112        16070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFYC010000112 DAZFYC010000000
VERSION     DAZFYC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.112"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKW5_003933"
     CDS             complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKW5_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO7513227.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2333..2920
                     /gene="betI"
                     /locus_tag="ACHKW5_003934"
     CDS             2333..2920
                     /gene="betI"
                     /locus_tag="ACHKW5_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO7513228.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2934..4406
                     /gene="betB"
                     /locus_tag="ACHKW5_003935"
     CDS             2934..4406
                     /gene="betB"
                     /locus_tag="ACHKW5_003935"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO7513229.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4420..6108
                     /gene="betA"
                     /locus_tag="ACHKW5_003936"
     CDS             4420..6108
                     /gene="betA"
                     /locus_tag="ACHKW5_003936"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO7513230.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPHTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLSPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKKMSRDVN"
     gene            6305..6973
                     /locus_tag="ACHKW5_003937"
                     /pseudo
     CDS             6305..6973
                     /locus_tag="ACHKW5_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7216..7911)
                     /locus_tag="ACHKW5_003938"
     CDS             complement(7216..7911)
                     /locus_tag="ACHKW5_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO7513231.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7904..9331)
                     /locus_tag="ACHKW5_003939"
     CDS             complement(7904..9331)
                     /locus_tag="ACHKW5_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO7513232.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEANGESFRSWFKKHLAQEKKNG"
     gene            complement(9342..10061)
                     /locus_tag="ACHKW5_003940"
     CDS             complement(9342..10061)
                     /locus_tag="ACHKW5_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO7513233.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10588..11442)
                     /gene="rclR"
                     /locus_tag="ACHKW5_003941"
     CDS             complement(10588..11442)
                     /gene="rclR"
                     /locus_tag="ACHKW5_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO7513234.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11668..12993
                     /gene="rclA"
                     /locus_tag="ACHKW5_003942"
     CDS             11668..12993
                     /gene="rclA"
                     /locus_tag="ACHKW5_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO7513235.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNQEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILHDQIFTHPSMSESLNDLFSLVK"
     gene            13102..13338
                     /gene="rclB"
                     /locus_tag="ACHKW5_003943"
     CDS             13102..13338
                     /gene="rclB"
                     /locus_tag="ACHKW5_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO7513236.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13350..13943
                     /gene="rclC"
                     /locus_tag="ACHKW5_003944"
     CDS             13350..13943
                     /gene="rclC"
                     /locus_tag="ACHKW5_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO7513237.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14103..14972)
                     /locus_tag="ACHKW5_003945"
     CDS             complement(14103..14972)
                     /locus_tag="ACHKW5_003945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7513238.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLAKFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            15221..>16070
                     /locus_tag="ACHKW5_003946"
     CDS             15221..>16070
                     /locus_tag="ACHKW5_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7513239.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVR"
BASE COUNT         4261 a         4054 c         3795 g         3960 t
ORIGIN      
        1 gctaatacaa aataaaatcg ttaattaata aaatataaaa tcatgaagtg atacaagagg
       61 aattgcagga tatgacatga caatcatata taatatatgc ataatttttt acagcgagtc
      121 aatgattccc gcgccatatg cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc
      181 gggaacatca cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc
      241 tcgtactggt caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg
      301 ccctggctgc cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt
      361 gcgcggtagg taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt
      421 tgttcttcgc ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc
      481 ggcaggcttt ttagctccac gtatgcgccg cgcaaccgca gctcctgcgc cacttcttcc
      541 attgccgggt aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc
      601 atcaggcgcg agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggt
      661 gcggtatcgc ggttggcact ttcacggcgg taatcttcta ccttcagaga tttatacaac
      721 cccgccatca cgaagaagat cacaaagctg aacggcagcc ccataatcac cgtggtgttt
      781 tgcagcgcgg atatcccgtt ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc
      841 gaccagaaga cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg
      901 aaattcccca gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta
      961 atggtggcga cggaggcgct aaaggtaaac gccggatact gcgccagcag gctgtaaaag
     1021 ccgcgctccg gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca
     1081 tacagcgcgc tattgccgaa caccgagagc cataacagcg tgaaggtaaa cggaataatc
     1141 aacgtgccca gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag
     1201 ccgacaaacg gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc
     1261 cactcaactg gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca
     1321 tagtcgccaa cattcagcac cagcgcatta agcaggaacg aggtgtcgcc cataaacaat
     1381 acgaacagga tcaatcccag cgccagcgca acgttaagtt ccgataacac acgaatcccc
     1441 ttatcgacac cggaggtgac agagatcgtg gcgattatca ccgacaaggc gatcagtgcc
     1501 gcttttgccg ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc
     1561 accacaccga taccgagcgt agtggcaata ccgaagatgg tgccgatcac cgctgcaata
     1621 tccactgagt gccctatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag
     1681 cggatggtga gcggcaaatt ataacgatag ctaaagtatc cgagcgccat gcccatcagc
     1741 gcatacatcg accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc
     1801 gcggcctcaa tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg
     1861 gctacggaga agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa
     1921 ctcagcaggc tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc
     1981 gaacaagcga tacagaccac aaagacaata tagagcgttg ccgccagtag atagtaccaa
     2041 ccgaaggttt tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga
     2101 aacaggatcg ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc
     2161 ggattgattt tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt
     2221 tattgttacc atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc
     2281 aatcaataac cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa
     2341 attggggatg cagtcgatcc ggcgcagaca actgatcgac gccacactgg aagcaataaa
     2401 tgaagtgggc atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac
     2461 ggggatcatc agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga
     2521 catcaccagt cagttacgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag
     2581 tgcagagcag cgattacagg cgattgttgc cggaaacttc gatgaaactc aggtgagcag
     2641 tgcggcgatg aaagcctggc tggcgttctg ggccagcagt atgcatcagc cgatgctcta
     2701 tcgtttacag caggtcagca gccgccgctt gctgtcgaat ctggtgagcg agtttcgccg
     2761 cgaattgccg cgcgaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg
     2821 attatggctg cgcgcggcac tgagcggcaa accgctggat aaacctctcg cacactcgct
     2881 gacccgtcac tttattactc agcatttacc caccgattaa ccgaggagac gtgatgtccc
     2941 gaatggcaga acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca
     3001 ccttcgagac cattaacccg gccaacggta acgtgctggc gaccgtgcag gccgccgggc
     3061 gcgaggatgt cgatcgcgcc gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga
     3121 tgaccgctat ggaacgctcg cgtattctgc gtcgggccgt tgatattctg cgtgaacgca
     3181 atgacgaact cgcaaaactt gaaaccctcg acaccggaaa agcatattcg gaaacctcaa
     3241 ccgtcgatat cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccggctc
     3301 tggaaggcag ccagatcccg ttgcgtgaga cgttatttgt ttatacccgc cgcgaacctc
     3361 tgggtgtggt ggcggggatt ggcgcatgga actacccgat tcagattgcc ctgtggaaat
     3421 ccgccccggc gctggcggca ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc
     3481 ttaccgcgtt aaagctggct gaaatttaca gcgaagcggg cctgccggac ggcgtattta
     3541 acgtgttgcc gggggtgggc gcggagaccg ggcaatatct gaccgagcat ccgggcattg
     3601 ccaaagtgtc atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg
     3661 cctcttccct gaaagaagtg accatggaac tgggcggtaa atcaccgctg atcgttttcg
     3721 atgatgcgga tctcgatctc gccgccgata tcgccatgat ggcaaacttc ttcagctccg
     3781 gtcaggtgtg taccaatggc acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg
     3841 agcagaaaat tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa
     3901 ctaacttcgg cccgctggtc agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca
     3961 aaggcaaaga ggaaggcgcg cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct
     4021 tcgataacgg cgcatgggtt gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca
     4081 tcgtgcgcga agagatcttc ggtccggtga tgtcgcttct cacttatgaa tctgaggacg
     4141 aagtgattcg ccgcgccaat gataccgact acggcctggc ggcgggcatc gtgacggcgg
     4201 acctgaaccg cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca
     4261 cctggggcga atccccggca gagatgcccg ttggcggcta caaacactcc ggcattggtc
     4321 gcgagaacgg cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga
     4381 tggctaaatt ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt
     4441 attggtgccg gctcagccgg caacgttctc gctacccgtc tgactgaaga tccgaatacc
     4501 tccgtgctgc tgcttgaagc gggcggcccg gactatcgct ttgacttccg cacccagatg
     4561 cccgcagcgc tggcgttccc actacagggc aaacgctaca actgggccta tgaaacagag
     4621 cctgaaccgt ttatgaataa ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga
     4681 tcgtcgctga tcaacggcat gtgctacatc cgtggcaatg cgctggatct cgataactgg
     4741 gcgcaagaac ccggtctgga gaactggagt tatctcgact gcctgcccta ctaccgcaag
     4801 gccgagactc gcgatgtggg cgagaacgac taccacggtg gcgatggccc ggtgagcgtc
     4861 accacctcca aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag
     4921 gcaggctacc cgcacacgga cgatctcaac ggctatcagc aggaaggttt tggcccgatg
     4981 gatcgcaccg tcacgccgca gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag
     5041 gccaaatcgc gccctaacct gaccattcgt actcacgcta tgaccgatca catcattttt
     5101 gacggcaaac gcgcggtggg cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc
     5161 gcaacggcca acaaagaagt gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg
     5221 caacgctccg gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat
     5281 gaattacccg gcgtcggcga aaatcttcag gatcatctgg agatgtatct gcaatatgag
     5341 tgcaaagaac cggtttccct ctcccctgcc ctgcagtggt ggaaccaacc gaaaatcggt
     5401 gcggagtggc tgtttggcgg cactggcgtt ggtgccagca accactttga agcaggtgga
     5461 tttattcgca gccgtgagga atttgcgtgg ccgaatattc agtaccattt cctgccagta
     5521 gcgattaact ataacggctc gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc
     5581 tcaatgcgct cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat
     5641 ccggcgattc tgtttaacta catgtcgcac gagcaggact ggcaggagtt ccgcgacgca
     5701 attcgcatca cccgcgagat catgcatcaa cccgcgctgg atcagtatcg tggccgcgaa
     5761 atcagccccg gcacggaatg tcagacggat gaacagctcg atgagttcgt gcgtaaccac
     5821 gccgaaaccg ccttccatcc gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg
     5881 gttgacggcg aaggccgcgt acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt
     5941 atgccgcaga ttatcaccgg gaatttgaac gccacgacaa ttatgattgg cgagaaaata
     6001 gcggatatga ttcgtggaca ggaagcgctg ccgaggagca cggcgggata ttttgtggca
     6061 aatgggatgc cggtgagagc gaaaaaaatg agtcgtgatg tgaactgact caagaaccga
     6121 tcagattgag agttaccgtt ccaggcatgg ggacagaatc gttatataaa acataggggt
     6181 aactctcatg cttcatatcc agtattcatg atgctggctt tttgctgata tttaacccga
     6241 acattcctga tacagactat taatacaacc ttatctgcga cagattttat tttctggata
     6301 tattatgcgt gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa
     6361 gaaaaaggtt cgtgttatcc tttttattat agctatctgt atggcaatga tgcttttatt
     6421 tctgtatatc aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac
     6481 accagacata cttgttaact gtgatagcaa taataacttt gcctgtcaga ccgcaatgac
     6541 taaagatgtt atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag
     6601 agaaataaag ctggaatggt catgagataa gagagattta ccaactgctg aatcggaaat
     6661 atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca
     6721 agttctcgat gagatacaaa taccttcagc aattaacagt gagctttata caaaaatgat
     6781 atacctgacc aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc
     6841 accagaaata aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag
     6901 cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa
     6961 ctctgggcag taataaacaa cattctgata gagagtgcct gtataaagct gaagtgacaa
     7021 tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt ctttttcact gtaaatatcc
     7081 tggcattact aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt
     7141 aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca
     7201 gatctcatat ctctctcagc aatcctcaat aatcagatac accgctttca ccgggccatg
     7261 aaccccgacg actttgataa gctcaatatc cgccgttgaa ctagggccgc taatgatgtt
     7321 aatgcaggac ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg
     7381 cgctacacgc ggcaggattg tgcttttacg caggataaaa agagaagatt ccgggagcag
     7441 gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc acgcctcccg attcggttaa
     7501 accatattca gcatacacga cacccacttt agcctgctct gcctgcgaga tattctcggc
     7561 acctttcgcc ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cgctaatccc
     7621 caattcctct agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca
     7681 cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat
     7741 aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gtgcggtaag
     7801 ccgctcgtta gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcaacgg
     7861 gcgacccagt gcctgagcaa cgttattcaa aaattcgctc cgattatcca ttctttttct
     7921 cctgcgccag atgtttctta aaccaactac ggaaactctc tccgttagct tcaggaagat
     7981 cgcgtgcttc catccagtca ctaatcgcgc caaatttgag tggtgttttg ccgccattga
     8041 taaaccagct tgccgcatga gcaccggcca tcatcccgac tttccacaat cctggatgac
     8101 tattggcata agcgaacatt tttatcgccc gttgctctgc ttttgcggtg atcccttttt
     8161 cagccatcac ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca
     8221 cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc
     8281 cgccaagtag cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat
     8341 gaccgccaat atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc
     8401 gcagcacatc ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca
     8461 gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt
     8521 tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta
     8581 cctcggcaaa cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca
     8641 gcgtggtaca cattcgcgca ttaccttcat tggtcaccag gcataccgaa cctgtctctg
     8701 ccaccgcgaa attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt
     8761 tttgccggat gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt
     8821 cgtgtagcac tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag
     8881 atggcggatc ttgatccagc tggagaatat attcacccag atcggtttca atcacctgaa
     8941 tgccagcatc ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt
     9001 tcaccacctt ccgggcattt ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt
     9061 ctttggtttt tgcaaaccag acgtgaccgc cgttttgcgt cactttttct gagagctggt
     9121 acagataagc gtcgagatta ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc
     9181 actcctccca gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct
     9241 gcgcgtttgc caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct
     9301 taaaatctgt attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact
     9361 tcagcaatat gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc
     9421 agcaggcaac tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca
     9481 accttttctt tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca
     9541 aatccgcagc aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc
     9601 agcagcgtaa gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga
     9661 tatactgctc tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata
     9721 aaagaggtga gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcaggt
     9781 tcatccgcca gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa
     9841 ataatgggat cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct
     9901 tctttgatat aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa
     9961 tttacgcgac agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc
    10021 agggcgtcac caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt
    10081 catgcccatt tatgcccgat gtttcccccc caccggggga tcaatcatca ataaatatgg
    10141 cgttcttttt taattggcta gcaacatacc acccctcatt tgtatgacaa atgatagtct
    10201 ttcatccaaa cttcaaatgc aataactaat tttatttctt tcggcatcgc tcacatttta
    10261 tggggaaatc gacgttcatt tttggcataa gaaatccatt taaaaaggct acatccctta
    10321 tacctattca gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa
    10381 aatcaccgac aataatcacg tatcaagtaa tagtcggggt tttatttaat tgaagcaatt
    10441 tagtttgcat ttttatttaa aaactgagct agagtccatc agaaaaaaat atataaagcc
    10501 caaaccaccg ttggaaatag aaatagttga aaggctcaca actcccggaa tgcggattat
    10561 ctttgccctg atttctggcg gttttattca gggtgcaagc tgtctgaccc tttcccgata
    10621 ttctcccgga gtacaaccaa actcgcggac aaacgccttg tgaaaagatg attcactggc
    10681 atagcctact gactcagcga tcaccacaac agggagcatt tcccgggaaa acatctgggc
    10741 cgctatttgt agacgcaact ttgttaatac agccagcggc gtggttccgg aaacatcacg
    10801 gaaaagctgg gcaaaacttg cccgggacat gtgagcgatg ctggccagcg attcgacggt
    10861 ccaggcgtgt cctggcattt ccagcatttg ctgtattacc gcaccaagac gtggatgcag
    10921 aagcaaactg agaatgtttt tctcagtatt aacctgtgca atccactcac gcaccgcgag
    10981 ggtaaagaat gtagcgcaga tctggctaca cagtgcatcc actcccggca tcgccgatct
    11041 ggattcctgt tgtaaaaacg ggatcgcctc cctcagccag ttatattcca cgctgtggtt
    11101 aaccggtgct aaaaacagcg tttccggcaa agacgttaaa aaataacgcg ccgaatgttg
    11161 caaccgaaga gtgccgcaga caatacaggt cgattcatta tcgacatgac tcaggcgatg
    11221 agctgaattt tgtggtagca ggaccacatt tcccgggcgt aatgtaaaaa tctctcccgt
    11281 cggcatttcc agcttcgccg ctccttgcgt taacgcatgc caacgaataa ccgataattc
    11341 cccggcacca tgtggaagct gccagtcact tcctaacacg caattcttat cgatcgttcc
    11401 ttgtggagcg ttaagcatca acaaacggct aagggcatcc atatcagact cctgagtggt
    11461 ttttgattac aaacaccaac aatcttagta aataactaat ctaataccat caaatggtta
    11521 atcatgattt ttgttttgtg attctgatta agaaaaagaa aataccagac gataagacaa
    11581 aatatcaagc gttgtgaaga aatggtattt gctcttttgc gtctatagtc atgatgtcaa
    11641 atgaacgcgt ttcgacagga aatcatcatg aataaatatc aggcagttat tattggtttt
    11701 ggcaaggctg gaaaaacatt agccgtcacg ctggcaaaag caggttggcg agtggctctc
    11761 atcgaacaat caaatgcaat gtatggcggg acctgtatta atatcggctg catcccaacc
    11821 aaaacattgg ttcatgacgc acagcagcac acagattttg tccgtgccat acagcgtaaa
    11881 aatgaagtgg ttaatttttt acgtaataag aattttcata atcttgcgga tatgcccaat
    11941 atcgacgtga tcgacggcca ggcggagttt atcaataatc atagcctgcg tgttcatcgg
    12001 cctgagggaa atcaggaaat tcatggcgag aaaattttta ttaataccgg tgcacaaacc
    12061 gtggttccgc caattcctgg aattaccacc acgcctggtg tatatgacag caccggatta
    12121 cttaatctaa aagaattgcc tgggcattta ggtattttgg gcggcggata tattggcgtt
    12181 gagttcgcct ctatgttcgc taattttggc agcaaggtaa ccattttaga agcagcttcg
    12241 ctgtttttgc ctcgggaaga tcgggatatt gctgataata tcgcgacgat tttacgcgat
    12301 cagggcgtcg atattatcct caatgcccat gtggagcgaa tcagtcacaa tgaaaatcaa
    12361 gtgcaagtgc atagcgagca cgcccaactg gcggtggatg cactgttaat agcttccggt
    12421 cgtcaaccgg ctaccgcttc gttacatcca gaaaatgccg gtatcgcagt aaacgagcgc
    12481 ggggcaattg tcgttgacaa gcaattacat accaccgcag acaatatttg ggcgatggga
    12541 gatgttaccg gcgggctgca atttacttat atttcactgg atgattaccg cattgtgcgt
    12601 gatgagttgc tgggagaagg cagacgtagt actgatgatc ggaaaaatgt gccttattcc
    12661 gtattcatga caccgccact gtccagggtt ggtatgacag aagaacaggc cagagagagt
    12721 ggtgctgata ttcaggtggt gacattgcct gtagctgcaa ttccgcgtgc cagagtgatg
    12781 aatgatactc gtggggtatt aaaagcgatt gttgataata aaacccaacg tatattaggg
    12841 gcatcactgc tgtgtgttga ctcccacgag atgattaata tagtgaaaat ggtgatggat
    12901 gccgggctgc cttatagcat attacacgat cagatattta ctcatccgtc gatgagcgaa
    12961 tcactcaatg atctattttc attagtcaaa taaactcaaa atcagacgcc agaacaaata
    13021 ttctggcgtc tcagagaaaa gaatcttatt aattccctgt tactctataa cttcttaatc
    13081 acttcattga tggtatttta tatgttcaaa aaatctgttt tatttgcaac actattatct
    13141 ggcgttatgg cattttccac caatgcagat gataaaataa ttctgaaaca tatcagcgtc
    13201 tcgtcagtat cagcatcacc gacagttctg gaggatacca ttgctgatat agccagaaaa
    13261 tataatgctt catcctggaa agtcacatcg atgcgaattg ataataattc aaccgcaaca
    13321 gcagtattgt ataaataagg atgttcacaa tggaaaaata cctgcacctg ttaagtcggg
    13381 gagataaaat tggcctgaca ttgattcgtc tgagtattgc aattgttttt atgtggattg
    13441 ggttattaaa gtttgtccct tacgaggcag acagcattac accattcgtc gcaaacagtc
    13501 cactaatgtc gttcttttat gaacacccgg aagactataa acagtatctg actcacgaag
    13561 gcgaatacaa accagaagca agggcatggc aaacggccaa taatacctat ggtttttcca
    13621 acggtcttgg cgtcgtggag gtgattattg ctctgctggt tttggctaat cctgtcaatc
    13681 gctggttagg tttattggga ggactgatgg catttaccac gccgctggta acactctcat
    13741 ttttaatcac caccccggag gcatgggtac ccgcattggg tgacgctcat catggtttcc
    13801 cttatttatc cggtgctggt cgcctggtat tgaaagatac tctgatgctg gcaggtgctg
    13861 tcatgataat ggcagattcg gcgcgggaaa ttcttaaaca acgcagtaat gaatccagtt
    13921 caacgttaaa aactgaatat tgataatccg aacactgtct gttgttcact ttttcaggcg
    13981 tcaggccccc tttctgatga tgaagcctga cgccatatta ccggagatat gacagagtga
    14041 agatcaatat cactcccctg ttaaaagatt cttgtcgtct caggtaaaat atatgattaa
    14101 tatcaatcat gtatttttac agcaagacaa ccacgaacaa attcaggatt aaaatgtttt
    14161 accgactccc caacataacc caaatcaagc gaactaattt gtgccatttc tttctctgtc
    14221 agtgagaaat cccagatagc aaaattttct tcgatacgct gttgatgtgt cgatttcgga
    14281 ataacgacca ctccccgttg aatattccaa cgcagaatga cttgagaaat cgatttttga
    14341 tgggcatctg caatactctg aagcagatta ttttcaaagg gtttatgtcg tccaccacct
    14401 aacggagccc atgcttcagg ctgcacgtta taatacttca tggttgccag cgcctctggt
    14461 tgggcaaaat agggatgcaa ctcgacctgg ttgaccatcg gtttaactct taccgtttca
    14521 caaaatttcg ccagaacatg aggatagaaa ttggaaaccc caattgcttt taatttgccc
    14581 tcttcatagg catcttcgag tgcacgccac gcactgaaat aatcgcgcat agcctggtgc
    14641 aataaataga ggtcgaagta ctctagtccc gattttttta atgatgcttc aatacctgct
    14701 gctgccgtat cctgattcaa catatcctgc acccacaatt tagacgttat aaataactca
    14761 tctcgggtac ataaaccttc agatatagct tcacgaacgg cttcaccgac ggcatcttca
    14821 ttaccgtata ccgctgcagt gtcgatgagt cgataacctg tgcggatagc gttcaacact
    14881 gactgtttgc acacgttttt atcggtaacc tgaaaaacac caaatcccat catcggcatt
    14941 ttcagattgt tacttaatac ggaaaattcc actgtcagtc cctccattag gcatgaacaa
    15001 tgagtctacg ttaaaacgta acctcaaatt agtacgtgga ttttgatatc acttatgcaa
    15061 aaaattcatt aataatgcag gactgaaacc tctctatttt cggggacaac gaagcagacg
    15121 ctaccagtgc ttttgccttc gcccttgctg tttttgatac acttagggcc cagggcataa
    15181 cgaaaatgtg cgatatgacg aagaatcaat ggagaatgtg atgatcaggc agaagattct
    15241 acagcagctc ctggagtgga ttgagtgcaa tcttgagcac cctatttcaa tcgaagatat
    15301 cgcacagaaa tctggctaca gcagacgcaa catccagctt ctgttccgaa atttcatgca
    15361 tgtgcctttg ggagaataca ttcgcaaacg aaggctttgt cgtgccgcca ttcttgtccg
    15421 gctcaccgcg aaatctatgc ttgatattgc actctctttg cattttgatt cacagcagtc
    15481 attcagccgc gaatttaaaa agttattcgg ctgctctccc cgtgtatacc gccaccgtga
    15541 ttattgggat ctcgcaaata tcttcccttc ttttttaata cgtcaacagc aaaagaccga
    15601 gtgtagatta gtcaactttc ctgagacacc tatttttggc aattcattta aatatgatat
    15661 tgaagtgtcg aataaatcac cggatgaaga agtcaaactg cgacgtcatc atttagccag
    15721 atgtatgaat aattttaaga cggatatcta tttcgtctcc acgtttgaac cgtcaacaaa
    15781 atcggtcgat ttgctcacgg ttgaaacttt tgccggtacg gtatgcaaac aaaccgattc
    15841 agagatgccg aaagagtgga caattaaccg aggactgtat gcctctttcc gctatgaagg
    15901 tgagtgggag cactaccctg aatgggcgcg aaacctctat ctgatggaat tacccgccag
    15961 ggggttagcc agagtgaacg gcagtgatat tgagcggttt tattacaatg aaaatttcgt
    16021 cgaaattgat agcaataaca ttgtttgcga aatttttatc cccgttcgtc
//
LOCUS       DAZFYC010000113        15812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFYC010000113 DAZFYC010000000
VERSION     DAZFYC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.113"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..294)
                     /locus_tag="ACHKW5_003947"
                     /pseudo
     CDS             complement(1..294)
                     /locus_tag="ACHKW5_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(593..757)
                     /locus_tag="ACHKW5_003948"
                     /pseudo
     CDS             complement(593..757)
                     /locus_tag="ACHKW5_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(883..1284)
                     /gene="eptO"
                     /locus_tag="ACHKW5_003949"
                     /gene_synonym="gspS"
     CDS             complement(883..1284)
                     /gene="eptO"
                     /locus_tag="ACHKW5_003949"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO7513240.1"
                     /translation="MRINTLKKLNCITSLLVLVTVAGCHQVPSTRKQATVPPSEQLEQ
                     TASIAAATRYLKRRCNRSDLPDDQAILNGVNRIANGKGWQSLTQEDIRKHSDEINERL
                     ARDSTPEHIKCSEFNRLLVPFIGELLAGTSR"
     gene            complement(1378..2136)
                     /locus_tag="ACHKW5_003950"
     CDS             complement(1378..2136)
                     /locus_tag="ACHKW5_003950"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO7513241.1"
                     /translation="MGSFLNVVIHRLPVILERRWRREALMLLAHSEPESEPVFNLMIP
                     RSHCPYCFQPLSIRDNIPLLSYLFLKGKSRCCGERIPIYYPLVEITSSVLFILAASRF
                     HPGLTLVAAWLFIAMLLVLAVIDCRTAMLPDVLTLSLLWLGLLFSLQGGRVPLEEAVV
                     GAVSGYLCLWGLYWLFRFVTGREGLGYGDFKLAAALGAWMGWQVLPSVLLFASVSGLI
                     ITVLLRFVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            complement(2263..2778)
                     /gene="gspM"
                     /locus_tag="ACHKW5_003951"
     CDS             complement(2263..2778)
                     /gene="gspM"
                     /locus_tag="ACHKW5_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO7513242.1"
                     /translation="MNDLKKRLQYISPRERRLLTGCVALLVVAFVYYVFWLPWQVRED
                     KWRTVIAREKYTVEWMKQQIPSIKKWEASMPEQGEPLGLSAAVTQTSAAYGVSVTRLQ
                     PQGERLTVTLAPVEFNTLMQWLTQLHRRYRIRTVVFDVVAQGNQPGHVTVNRLVLSRD
                     DINRDTSPGHL"
     gene            complement(2765..4042)
                     /gene="gspL"
                     /locus_tag="ACHKW5_003952"
     CDS             complement(2765..4042)
                     /gene="gspL"
                     /locus_tag="ACHKW5_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7513243.1"
                     /translation="MERGAFPEHSATVWINGEGGTLNKNNKHVEAGRLFLRLPEDDTQ
                     PLLWRLVSPEETGGGSVAVGETDPLLAGLLPRYPAWVLVPSDRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQTGGRCNVAVVEKSVMHHWLTYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVRLAGQWLFRSDRYAGMTVELSWLTQFLSLTAPPVIESYSV
                     PPAQCMGLESTEWRTQPVRDLLQLAAECEGYDGADLRQGIFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAEFWQHESVRVYQELFPGETVVKDPHRQMLRHLQ
                     QSTSNNMPELGGVMHQLQQLLPETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     SAGEKYIIQPGEIRQQPHGVESRLILRMNNERS"
     gene            complement(3976..4953)
                     /gene="gspK"
                     /locus_tag="ACHKW5_003953"
     CDS             complement(3976..4953)
                     /gene="gspK"
                     /locus_tag="ACHKW5_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7513244.1"
                     /translation="MKSREQGVALLVVLLILSLMVTIAAAIAERNGRTFWRTVAQLDQ
                     LQAKWDGYAAEEMAIQILQRSRQASPRKTHLAQNWAQPELQFETGGGDVRGRIVDAQA
                     CFNLNAINYGAVDLAGVPYAARIFQQLLINLQVDIIQARQVTAALRDWIDRDDEPVKE
                     GAEDEVYMGMEPPYLAASQPMQDVSELRLIRGIDVRLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPNSLLVTKLLLRRPRTGWDSVAAFLDSPQLKGIDTSTAISV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRSIQRQYGLTVREVP"
     gene            complement(4950..5549)
                     /gene="gspJ"
                     /locus_tag="ACHKW5_003954"
     CDS             complement(4950..5549)
                     /gene="gspJ"
                     /locus_tag="ACHKW5_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7513245.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQSGIRAHELSQD
                     KVRRLAELQRGGSQIERDLMQMIPRHSRGSEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFYVDHPSGIAPDVRVVLEGVHAFRLRFFVNGT
                     WQARWDSTSILPQAVEVTLVMDDFAELTRLFLVSKETAE"
     gene            complement(5546..5911)
                     /gene="gspI"
                     /locus_tag="ACHKW5_003955"
     CDS             complement(5546..5911)
                     /gene="gspI"
                     /locus_tag="ACHKW5_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7513246.1"
                     /translation="MKRQEGMTLLEVMVALVVFALAGIALIQSTTQQAAGIGPMEEKV
                     LAGWLADNQMVQLQLEKIWPKNGWDEKTISFAGREWYLRWHGRDSDIPEQRILEVEVR
                     RTREETSALVSLRGSVVRE"
     gene            complement(5908..6459)
                     /gene="gspH"
                     /locus_tag="ACHKW5_003956"
     CDS             complement(5908..6459)
                     /gene="gspH"
                     /locus_tag="ACHKW5_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7513247.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSMQDGLLIGIQVRSDGWTFKVLQRVTAESFPASVGGDVWQGYVWQTWQ
                     PRRAAMEGKLPDDVRLDLQYLRGLQWMPEHNDGTGPDILLLPGGEVTPFSLLFRLVGG
                     EAVTGLQVDENGLMTLFEGEVEL"
     gene            complement(6456..6890)
                     /gene="gspG"
                     /locus_tag="ACHKW5_003957"
     CDS             complement(6456..6890)
                     /gene="gspG"
                     /locus_tag="ACHKW5_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7513248.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     VSDLVALESALDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSNIDIFSPGPDGIPNTEDDIGNWTPGHTQP"
     gene            complement(6921..8144)
                     /gene="gspF"
                     /locus_tag="ACHKW5_003958"
     CDS             complement(6921..8144)
                     /gene="gspF"
                     /locus_tag="ACHKW5_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7513249.1"
                     /translation="MALFHYQASDIHGRKRRGVLEADSARHARQLLREQALIPVRLEE
                     KQLHHQHSPRSILRFRPRGVSSAELALLTRQLSTLVAASLPLEEALDALSRQSEKPRQ
                     RDLIAAVRARVLEGHSLATAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALIYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGL
                     TEVCQTWGPWLLLAAATGWIAGRMMLHKPSRRLAFHHLLLRLPIVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMYISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPS
                     MMRHMIASGENSGELDSMLERAADNQDREFSAQMQLALGLFEPMLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNI"
     gene            complement(8146..9648)
                     /gene="gspE"
                     /locus_tag="ACHKW5_003959"
     CDS             complement(8146..9648)
                     /gene="gspE"
                     /locus_tag="ACHKW5_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7513250.1"
                     /translation="MSSIQNVSESRPLLPFSFSRTQRILLLREQEGNQVFCMEDTPAS
                     ALLEVRRVAEGPLNVTTLSAEAFEKQLVSCYQRDSDEARQMMADIGNEMDFYTVAGEL
                     PDSEDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRVDGVLREILR
                     LRRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIVL
                     RLLDKNSVSLDLAALGMSPQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSRLN
                     PQDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDSET
                     AQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLCP
                     RCRQSCQASAELAKDMDIPPETTIWQPAGCQHCSFTGYHGRTGLHELLLIDDRIRMAI
                     YQGEGELSITRLAGGRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            complement(9645..11615)
                     /gene="etpD"
                     /locus_tag="ACHKW5_003960"
     CDS             complement(9645..11615)
                     /gene="etpD"
                     /locus_tag="ACHKW5_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO7513251.1"
                     /translation="MFRKWLNGRLSIFVFTTVFLSAIPGWGAEFSASFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDASPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVVKRLMEIVERVDKEGNRSVATVPLTYASATDVARLVTELTKES
                     DKTAMPAWMTAKLVADERTNSVLVSGEPISQQRIISMIKQLDRKEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSNIENDAKKSSSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEVQDADGLNLGIQWVNKHSGVTQFTSTGLPITTMVQTR
                     QSDISDSDQSNTLSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFSSKSEHAAKRNLMLFIRPTIIRERDGFRHASAEKYLSFNQEQVQSRGKETMALTL
                     NEEQLKLSRDRDDTTFRKVKTAIAAFYARGMQ"
     gene            complement(11616..12491)
                     /gene="gspC"
                     /locus_tag="ACHKW5_003961"
     CDS             complement(11616..12491)
                     /gene="gspC"
                     /locus_tag="ACHKW5_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7513252.1"
                     /translation="MLFFLSSRRDRNLFIKDIALKMLTPNWVLCVILLIAGYQLVSVI
                     RHFWLTPATSASDLSHVSVSETAVTDEHTEENFVFTLFGTASPPLSEGKVQKTTSSLS
                     DDLLSGGDLDVRGILYSSVTEHSVAIFAHNNRQFSLGIGEKVPGYDATISAIFSDHIV
                     INYQGKNASLPLRYDNPAKRNAQDDNNLIVGPVTTQANFRVKNIFDIMSLSPVTVNNT
                     LSGYRLSPGKASSLFYNAGLHDNDLAVLLNGSELRDTRQAKQIMKQLTELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            complement(12575..15235)
                     /gene="stcE"
                     /locus_tag="ACHKW5_003962"
     CDS             complement(12575..15235)
                     /gene="stcE"
                     /locus_tag="ACHKW5_003962"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO7513253.1"
                     /translation="MKLKYLSCMILASLAMGAFAATAADNNSAIYFNTTQPVNDLQGG
                     LAAEVKFAQSQILSAHPKEGESQQHLTSLRKSLLLVRLVKADDKTPVQVEARDANDKI
                     LGTLTLSPPSSLPDTVYHLDGVPADGIDFTPQNGTKKIINTVAEVNKLSDASGSSIKS
                     YLANNALVEIQTANGRWIRDMYLPQGAELEGKMVRFVSYAGYNSTVFYGDRKVTLSVG
                     NTLLFKYVNGQWFRSGELENNRIAYAQHTWSAELPAHWIVPGLNLVIKQGNLSGSLND
                     INVGAPGELLLHTIDIGMLTTPRGRFDFAKDKEAHREYFQTIPVSRMIVNNYAPLHLK
                     EVMLPTGTLLTDADPGEGGWHSGTMRQSIGKELISHGIDNANYGINSTAGSGESSHPY
                     VAAQLTAHTNRGNYSNGVQVHGGSGGGGIVTLDSTLGNEFSHEVGHNFGLGHYVGGFR
                     GSVHRSADQINSTWGWDSDKKRFIPNFYPAQTNRKSCLDEQCQEPFDGHSFGFDAMAG
                     GSPFSDANRFTMYTPNSSAIIQRFFENKAVFDSLSSTGFSKWNADEHKMEPYEHTIDR
                     FEQITASVRDLSENKMAEMMAEYPIVKVHMSDGNWTRDIHAPAASAENKGRILTLDHE
                     AGYNSHLFINGGEKIISKGYKKSFVSDGQTWKESDVVNTREARKPEKFGVPVTTLVGY
                     YDPKGTLSSFIYPAMYGAYGFTYSDDSENLSSNDCQLQVDTREGQLRFKLANNRVNSS
                     VMNKFHINVPTESQPTQAILVCNNKVLDTRTLTPAPEGLTYTVNGRALPAKENEGCIV
                     SVKTAQRYCLPVGQRSGSSLPDWIAGQEVYVDSGAKAKVLLSDWDNLSYNRIGEFVGN
                     VNPADMKKVKAWNGQYLDFSRPRSMRVVNK"
BASE COUNT         3987 a         4477 c         3496 g         3852 t
ORIGIN      
        1 ctattctccg gtcatctgca gcagtttttt ggcatcgtga cttctgagca acggaatgcc
       61 agcctcataa cctcttccag actaatatcc cggcgccagc gttccagtcg aaaatgacag
      121 gtatcatcca gctctactgc tgcaacaata ctgaacgagc gccgttcaac ggacgatatc
      181 agcccgtacc tgcggacaca gataccacca tttcatatcc acgattttgg acagtccttt
      241 cataaaactg acttcccttt ctgtcagcag cgtcttctga cctcaccagt attcactctg
      301 catcaccgat gccgtataca tgaacaagac aaattggcgt gatggttatg tgaaagagga
      361 gaaaatgccg atgtttcagt taggtattga tgtcagtaaa aacaatcttg atctatgcct
      421 cttacggggg acattaaagg gaagatcaaa actcgtaagc taaaaaatac cgctgatgcg
      481 gctaaaactg tggcagagtg gcttaaaaag caatccagtg aactgaacga agttcatatc
      541 atcatggaag ccactggggt atatcatgaa cctctagctt atggccttca ctttgctggc
      601 gctcagtggc agttagtcca tcattaccgc tcccggtagc ccgctcatgc tggcgccccc
      661 agacacgtag agtttctggc gtacagctaa tctttggggc aatgaaacaa attgccgccc
      721 gttgtaagtc atattcgccc tgacgcacca gaatcatacg gaccgtccgc taacggactt
      781 ccggggaaaa acgtgtattt ttggtcatcc tgtttacctc tttctcaggg agtttagtct
      841 cctagatttc cgaggcgcgg ttcagtacct gtcagtaccc tattatctgc tagtccctgc
      901 cagtaattca ccgataaatg ggaccaacag ccgattgaac tcactgcatt ttatgtgctc
      961 aggtgtgctg tccctagcca gacgttcatt gatctcatcg ctgtgtttac gtatatcctc
     1021 ctgagttaag gactgccacc ctttcccgtt agcgatccgg ttaacgccat tcagtatcgc
     1081 ctgatcatct ggcaaatcac tgcggttaca tctccttttc agataccgtg tcgcagcggc
     1141 gatcgacgcc gtttgttcta gctgttcgct cgggggaacg gtggcctgct tacgagtgga
     1201 aggtacctga tggcaaccgg cgacagtcac caggaccagt aatgacgtga tacaatttag
     1261 ttttttcaac gtattaatcc tcatattttt aaccaggtat tgtttaaccg gtttccggtt
     1321 tactatagtc ccgttattct ctgagccatt tttattacca gccccggaaa ccgaacgcta
     1381 catcaacaaa aaatggcata ctccgctcaa ggcgagccac ggaccaaaag gcagcggccg
     1441 ggtaaaatcc gtggctgtaa caaaacgcag cagtaccgtt atgattaatc cgctgactga
     1501 ggcaaacaga agcacggagg gtaatacctg ccaccccatc catgctccca gtgccgcagc
     1561 tagcttaaaa tcaccgtagc ctaacccttc tctaccggta acgaaacgaa aaagccagta
     1621 cagcccccac agacagagat acccagagac cgcccccact accgcctcct ccaagggcac
     1681 tctccccccc tgtaagctga ataacaggcc aagccagagc agggataacg tcagcacatc
     1741 cggcagcata gccgtacgac agtcaataac cgccagaacc aacagcatgg caataaaaag
     1801 ccaggcagca actaatgtca gcccgggatg aaaccggctc gctgccagaa taaataacac
     1861 tgaactcgtt atctcgacca gcggatagta aataggaata cgctcaccac aacagcgtga
     1921 tttccccttc agaaagagat agcttaacag gggaatgtta tccctgatgc tgagcggctg
     1981 aaaacagtag ggacaatgag agcgcggtat catcaggtta aagaccggct cggactcagg
     2041 ctcagaatgt gccaacaaca tcagcgcttc acgccgccac cgcctctcca gtattaccgg
     2101 cagacgatga atcacaacgt taagaaaact accgataatg ccccctagcc cccctgtgct
     2161 gatgagccac aacaaatgaa aatcagccgc aaattccggc aacctgctca catatcctcc
     2221 tgcttatcac tcccattttc acgtcaggca ttaacctgta aatcacaagt gccccggcga
     2281 agtatccctg tttatgtcat cccgggacaa aaccagccga ttaaccgtta catgtccggg
     2341 ctggtttccc tgcgcgacca catcaaatac cacggtacga atacggtacc tgcgatgcag
     2401 ttgcgtaagc cactgcatca gcgtattaaa ttcaactggt gctaaggtaa cggtcagacg
     2461 ctccccctga ggctgcaatc gggtgactga tacaccatat gcggctgaag tttgagtcac
     2521 tgccgcagat aaacctaacg gctccccctg ttctggcata ctggcctccc atttctttat
     2581 gctgggtatc tgttgtttca tccactccac tgtgtatttt tcacgggcaa ttacagtccg
     2641 ccacttatcc tcccgtacct gccagggcag ccagaagaca taatagacaa aagcgacaac
     2701 aagtaaggca acacatccgg taagcagcct tcgctcacgg ggactaatat actgcaaacg
     2761 ttttttaaga tcgttcattg ttcattctca ggatcagacg actttccacc ccgtgcggct
     2821 gctgccgtat ctcgccaggc tggatgatat atttttctcc agcgctctgc tgaaagtgtt
     2881 ccagagcctg aaaagaggct gcctgcagat ctattttcag agtctggctg ctactgtccc
     2941 aggcgagtgc ctgtaagcgt atcgttgagg tttctggcag cagttgctga agctggtgca
     3001 tcacgccccc tagttccggc atattgttac tcgtcgactg ttgcagatgt ctcagcatct
     3061 gccggtgggg atccttcacc accgtttcgc caggaaacag ctcctgatag acgcgtacgc
     3121 tctcatgctg ccaaaattca gcctgctgcc agagccggta atgtgccagc cccgcgttgc
     3181 ttccggccag aaacacacag gccaacaggg cccctgcgac ataccgccat gggcggaatc
     3241 gcgcatacca tgttccctgg cgagcaaata ttccctgtcg cagatctgcg ccatcatatc
     3301 cctcacattc agccgccaac tgcagcagat cccgtactgg ctgagttcgc cactccgtgc
     3361 tctccaagcc catgcactgt gctggcggca cggagtaaga ctcaataacc ggcggcgcag
     3421 taagagacaa gaactgtgtc agccacgaca actccaccgt catcccggcg taacgatcac
     3481 tacgaaatag ccactgaccg gcaagcctta ctgcactcca tccttcagtt gccaacggca
     3541 gcatcagtac gtcaggcagc aacctcacct cccggacccc aagttgtcca cagtacgtca
     3601 gccagtggtg catgaccgat ttttcgacca cagccacatt acacctgcca cctgtctgat
     3661 gaagaatggc gaaatgcagc tgctcaatat ctgttgccaa ctgctcttcc agaagaaagg
     3721 ggagcacctg caaacgctga cgccacgtac ggtgtggcag cgtgacctga tggaaggcaa
     3781 ttctgtctga cggaaccagc acccaagcgg gataacgagg cagcagacct gctaacagcg
     3841 gatctgtttc tccgactgcc acgctgccgc cccctgtctc ctccggcgaa accaacctcc
     3901 acaataaagg ctgcgtgtca tcctccggca gtcgcaaaaa aagccggcct gcctcgacat
     3961 gtttgttatt cttgttcagg gtacctccct caccgttaat ccatactgtc gctgaatgct
     4021 ccggaaacgc tcctctctcc actgcattaa agtctgctgg ctgaacaggt gctccccaga
     4081 gcgcacatgt aaccggacca aaaaatagtt gctgctaaca gccagtaccg atatggctgt
     4141 actggtgtcg atgcccttaa gctgtggcga gtcaagaaac gctgccacgc tatcccaacc
     4201 ggtacggggt cgccgcagca gtaatttcgt caccaaaaga ctgttcggtt tcgtcagaaa
     4261 gagtgcagcc agcaacggcg cctgtgaatc cagcagagta ttcacattca ctgacaggtc
     4321 tgacgtgggc agtacacaaa catacggtaa aagtttgcgg taaagtctga catctattcc
     4381 ccggatgagg cgcaactcac tgacgtcctg catcggttgg ctggccgcaa gatagggggg
     4441 ctccatcccc atatacacct cgtcttcagc cccctcctta actggttcgt catcacgatc
     4501 gatccagtca cgcagagctg ctgtcacttg cctggcttgt ataatatcga cttgcaggtt
     4561 gattaacagc tgctgaaaaa ttctggcggc ataaggcaca ccggctagat ccactgctcc
     4621 ataattaatg gcattaagat taaaacaggc ctgtgcatcc actatccgtc cccgaacatc
     4681 tcctccgccg gtttcaaact gtagttctgg ctgcgcccag ttttgtgcca gatgcgtttt
     4741 ccggggcgat gcctgtctgc tcctctgtaa tatttgtatt gccatctcct ctgcagcata
     4801 gccatcccac tttgcctgca gctgatccag ctgcgccacc gtacgccaaa atgtcctgcc
     4861 gttacgctcc gcgatcgcag ccgcgatcgt caccattaac gacaagatca gcaatacaac
     4921 cagaagcgcc acgccctgtt cccgcgactt cattccgcgg tctccttact tacaagaaac
     4981 agacgggtaa gttcagcaaa atcatccata accagcgtca cctcaacagc ctgcggcagg
     5041 atgctggtac tgtcccagcg cgcctgccaa gtgccgttga caaaaaaacg cagacgaaat
     5101 gcgtgaaccc cctccagcac caccctgaca tccggagcaa taccagaggg gtgatcgaca
     5161 taaaagtagc tcaggcgttc taacttttgc tgtcgcagcc ggtaacctac ccactgtaac
     5221 tccgggcgtg gtagcatccc ggcgggatta agccaactgt ttcgcgtaaa ggaaatcccc
     5281 cagtcatcgc ttttcagcag atgaggagca gcgagcagca ggccttcact cccccggcta
     5341 tgtcttggga tcatctgcat aagatctcgc tctatctggc tgccccccct ctgcagctca
     5401 gccaaacgcc gtactttatc ttgggacagc tcgtgcgcgc gtattccgct ctgtagtacc
     5461 tgaaatgcgc tgatgctcag cgctgcaaaa acagccagtg ccaacagcat ttccagtagc
     5521 gtaaaacctt tcacccgctg ctgactcatt cacgaaccac actaccacgc aaggaaacta
     5581 aggcggatgt ctcctctctg gtacgccgta cctctacctc taggatgcgt tgctctggta
     5641 tgtcgctgtc cctcccgtgc cagcgtaaat accattctct gccagcgaag ctgatcgtct
     5701 tctcgtccca gccgtttttc ggccagattt tctcgagctg cagttgcacc atctggttgt
     5761 cagccaacca gccagccaac accttctctt ccatcggccc gatccctgcg gcctgctgag
     5821 tcgtggactg aattagagca attcctgcca gcgcaaacac aaccaaggcc accatgactt
     5881 ctagtaatgt cattccttct tgcctcttca caactcgacc tcaccttcaa atagggtcat
     5941 caggccgttc tcatcaacct gcagcccagt aacggcctcc cctcctacca gcctaaagag
     6001 aaggctgaac ggcgtcacct cacctccagg cagcaacaaa atatcgggcc ctgtcccgtc
     6061 attatgctca ggcatccact gcagacctct gagatactgc agatcaagac gcacatcatc
     6121 cggaagtttg ccctccatgg cagcacgccg cggttgccaa gtttgccaga catacccttg
     6181 ccaaacatcc ccccccactg atgccgggaa gctttcagct gttacgcgct gcaaaacctt
     6241 aaatgtccac ccgtcagacc ggacctggat cccaatcagc aagccgtcct gcatgcttct
     6301 atccaaggca taatccagct gcgcctgcag acgctcccgc tgttgttgca aatgattctg
     6361 cgctgagtca ggaaatgaca ttagtaccaa ccctgcagta atgcccatca acaaaaggat
     6421 cagcatcatc tccagtattg tgaatccgcg ctgactcacg gctgcgtgtg accgggcgtc
     6481 cagttaccaa tatcatcttc cgtgttcggt ataccatccg ggccgggaga gaagatgtca
     6541 atattgctgt actgaccagg atttaacagc tgataatccc ccccccaggg atcttgaggc
     6601 aaacgacgta tataaccatc ttgccgatag ttacggggct caggctgcac tgtaggttta
     6661 ctgaccagtg cccgtagccc ctgctccgtt gttgggtaac ggttgttatc taaccgatac
     6721 atatcgagcg cgctttccag cgcaaccaga tcgctaacca ctttctgccg atcggcctta
     6781 tccttatttc ccatcagatt cgggaccacc agactggcca gaactcccaa gatcacaatt
     6841 accaccatta tttccaacaa ggtaaaacca cgctgatgct gttttcgcat gtttttctcc
     6901 gttgactatt aaagaagaat tcaaatattc atcatattgt tgagttgcag gagcggttgc
     6961 agaattgcca gaacaataaa caagacgacg ccagccatcc ccaccaccaa cattggttca
     7021 aacaatccca gcgccagctg catctgtgcg ctgaattcac gatcctgatt atcggcggcc
     7081 cgttccagca tgctgtcgag ttcgccacta ttttcaccgc tggcaatcat gtgccgcatc
     7141 atggacggaa atagcgaggt ctgctccagc gcctgatgca ggctgacccc ttcccgaacc
     7201 aattcggctg cggtggctaa ctgatgacgg gcccagtcat tactcaacac gtctccactg
     7261 atgtacatag cctggagtag cggcaccgca ctggcattaa ggatgctcag cgtgcgggca
     7321 taacgtgcgg tattcagccc acgcgatata cgccccacga tcggcaggcg cagcagcaga
     7381 tgatgaaaag ccagacgccg ggagggcttg tgtaacatca tacgccccgc tatccagccc
     7441 gtggctgcgg ctagcagcag ccacgggccc caagtctggc agacctcagt cagccccatc
     7501 agtacgcggg tagacagagg aagggtctgt ttcatatgaa taaactgctc aaccactttc
     7561 ggcacaacag cagtaagcag aatggcaatc acccctaccg ctaccaacgt caggacacag
     7621 ggatagatca gtgcctggag cagacggttt cgcatgatct ggcgttgctc ggtatagtca
     7681 gccagacgac taagtaccac atccaagcga ccggatgttt cacccgccgc caccatcgca
     7741 caataaagac gctcaaacgt accaggaaac attcccatcg ccgtggccaa ggaatgtccc
     7801 tcaaggaccc tagcgcgcac ggcagcgatt aaatcccgct ggcgaggttt ctcactctgc
     7861 ctcgacagcg cgtccagcgc ctcttcaagc ggcagtgatg cggccaccaa ggtggataac
     7921 tgccgtgtca ataatgcaag ctcggcgctg ctcaccccac gaggacgaaa tcgaaggata
     7981 ctccgcggtg agtgctggtg atggagctgt ttctcttcca gcctaactgg aataagcgcc
     8041 tgctcacgca gcaactggcg ggcatgtcga gctgaatccg cctccagaac gccacggcgt
     8101 tttctaccgt gaatatccga tgcctgataa tgaaacaacg ccatatcatt cctcttcctg
     8161 caacctgctt tcagtaacgc gaaccacctc ctcccaactg gtctcaccgg ctagcacctt
     8221 ttgtcgcccg tcgccacgca gcgtcagata gcgcccccca gccaagcggg taatactcag
     8281 ctctccttcc ccctgatata ttgccatccg gatgcgatcg tcaatcagca acagttcgtg
     8341 gagcccggta cgcccgtgat agccagtaaa actgcagtgc tgacacccgg caggctgcca
     8401 aatcgtggtt tccgggggaa tatccatgtc cttcgccagc tcagcactgg cctgacagga
     8461 ctgacgacag cgcggacaca gcgtgcgaac tagtcgttgg gctagaacag ccaacaatga
     8521 ggtggaaagc agaaaggggg ggatgcccat atcctgcagg cgcgataacg cgcctgcagc
     8581 gctgttcgta tgtaacgttg acagcaccag atgcccagtc agtgaggcct gtacagcaat
     8641 ctgggctgtt tcactgtcac ggatctcccc tacaagcacc acatctggat cctgacgtaa
     8701 aatagcacgc aggctcctgg caaaagtcat gtccaccttc gggttgacct gggtctgact
     8761 gataccgtcc agttcatatt caacaggatc ctctactgtc atgatgttgc ggtcctgtgg
     8821 attcagccgg cttaacgcag cataaagcgt cgtgcttttc cccgacccag ttggcccagt
     8881 caccaaaata atgccgtgag gacggcggat cagcgcatca atgtgtctct gattctgcgg
     8941 cgacatgccg agtgcagcca gatcgagact aacgctgttt ttgtctagta aacgcagcac
     9001 gatgcgttca ccatggttcg aaggcaacgt tgaaacacgc acatccacag cccgtccgcc
     9061 tatacgcagc accatgcgac catcttgggg aacgcgtttt tcggcaatat ccagccgagc
     9121 cataacttta atgcgtgaaa tcagtaacga agccagattc cggcgtaaac ggaggatctc
     9181 ccggagaacg ccatcaacgc gaaagcgaac ctgtagatgg cgttcgtacg tttcaatatg
     9241 aatatctgag gctttctctt taattgcctc agtcagcatg gcgttaatca ggcggataat
     9301 aggcgcatcg tcattcgcat ccagtaagtc ctcactgtca ggcaactccc ccgcgacggt
     9361 ataaaaatcc atttcattac caatatcagc catcatttgt cgcgcttcat cagaatcacg
     9421 ctgatagcag ctcaccagct gtttttcaaa tgcctcagcc gacagagttg tgacattcag
     9481 aggaccttca gctaccctgc gaacctctag cagggcactg gcaggtgtat cctccatgca
     9541 aaacacttgg ttcccttcct gttcacgcag caacaaaatt cgctgtgtac gggaaaagga
     9601 aaaagggaga agggggcgtg attcactgac gttctgtata ctgctcattg catcccccgc
     9661 gcataaaacg cagcaatcgc cgttttcact ttccgaaaag tcgtatcgtc ccgatctcgg
     9721 gatagcttga gctgttcctc attcagcgtc agcgccattg tttctttacc acgcgactgc
     9781 acctgctcct gattaaacga caggtatttt tcggccgaag catgacgaaa tccgtcgcgt
     9841 tcacgaataa ttgttggccg aatgaacaac atcagattac gcttggctgc atgttcactt
     9901 tttgagctaa aaagatgtcc cagtactggg atatccccca gcagaggtac tttactctca
     9961 ctccctctga tcgacttatc cagtaatccg ccgaccacca ccgtttcgcc attccctacc
    10021 agcatagcat tggtcactgt ccgggtattg aaggttgccc ccaagtcagt agtggtagct
    10081 actgctgtat ctgccacacc ggacacctcc tgctctatct ccagtaacac tgaatcaccc
    10141 tcgttgatct ggggttttac cctgagtttg atccccaccg ttttacgctc gacggtgtta
    10201 aaaatattgt ccccagaggt ggtctgtgaa cccgaaagca ccggaacttc ctgaccaaca
    10261 ttgaacgtgg cctccatatt gtccagcgta acaatactgg gggttgccaa gatatcattt
    10321 ttgctacttg tggagagcgc cgtcagcagc atcgcccagt ttccctgata aaaccctgct
    10381 gcaatcccgt taaacatgct caaggtattg ctctgatcgc tgtcagaaat atcgctctga
    10441 cgggtctgaa ccatcgtggt aatgggtaaa ccagtactgg tgaactgcgt cacaccggag
    10501 tgtttattca cccactgaat ccccagattc aacccgtcag catcctgtac ttcagcaatg
    10561 atggcttcca ccaggacctg aggacgacga atatccaact gcgaaattac attttccaag
    10621 tcgcggatga cgtcaggagc ccctgtcagg atcaaggcat tggtttgttc gtgggattta
    10681 atcgtcaccc cttttcgcag agcttccgtt gacgagctct ttttagcgtc gttttcaata
    10741 ttgctgctga tgcccgtcag gacttccact aaatccttcg ccttcgcata tttcaggtaa
    10801 atcaccttcg tattaccctg aacatcctcc ttacggtcca gttgcttaat catggagatg
    10861 atacgctgtt gggagattgg ctctccgctg acgagaactg agtttgtccg ctcgtcagca
    10921 accagcttcg ctgtcatcca ggctggcatg gctgtcttat cactttcttt agtcagttcc
    10981 gtgacaagtc tggctacatc cgttgcagag gcgtaggtaa gtgggaccgt ggcaacgctg
    11041 cgattgccct ccttatccac ccgttcaaca atctccatca gccgtttcac cacggcagca
    11101 cgaccagtca tcagcagaac gttagagggt tcataatgca ctacgcttcc tgcgccagca
    11161 ttatcattga gctgacgcag caagggcgcc aagtctctgg ctgccacgtt actgacgggg
    11221 accacccggg taacaacctc atcaccagtc ccagggctgg catcactagc taccggcacc
    11281 gccgacgttt tagcatcttt tgaacgcacc actttcagta taccgttgtg catatcgacc
    11341 acggcaaaac cataaacatc cagcacgctg agaaagaact gataatattg ttcctcgttc
    11401 agcatatcgt agctgcgtac agtgatggtc ccctgcacat caggattaat tattaccgtt
    11461 ttgtgcagat ttttgctgac agtattaatg aactcctgaa tatccgtatc tttaaagctg
    11521 gccgaaaatt cagcccccca tcccggaatg gcactcagaa atactgtcgt gaatacaaat
    11581 atcgacaagc gcccgttcaa ccatttcctg aacaatcagt tctctcctac agcaataaag
    11641 gcatcataca gttgaccatc acgttcgacg gttattttta tttctttaag ttctgtcaat
    11701 tgtttcatta tctgcttcgc ctgtctggta tcacgcagtt ctgaaccatt aagcaatacg
    11761 gccagatcgt tatcatgcaa cccagcatta taaaataacg aactggcctt accggggctc
    11821 aagcgataac cgcttaatgt attattcacc gtgacaggtg aaaggctcat tatgtcaaaa
    11881 atatttttta cccgaaaatt agcttgggtt gtcacgggtc cgactattaa attattatcg
    11941 tcctgtgcat tcctttttgc tggattatca tatcgcagcg gcagtgatgc attttttccc
    12001 tgatagttaa taacaatatg atcgctaaaa atagcactaa tcgtagcatc gtaaccaggt
    12061 actttttcgc cgatgcctag actgaactgt ctgttattat gtgcaaatat ggcaacggaa
    12121 tgctctgtaa ccgaactgta aagtatacct ctcacatcaa gatccccccc tgaaagcagg
    12181 tcatcagaca gggaagatgt tgttttctgc actttccctt ccgataacgg cggggatgcc
    12241 gtgccgaata acgtaaacac aaaattttct tctgtgtgct catcggttac cgctgtttcc
    12301 gacacgctaa catgggataa atcggacgcc gatgtcgccg gagtcagcca gaaatggcgt
    12361 ataaccgaga ccagctgata tccggcaatg agtaaaatca cgcacaacac ccagtttggc
    12421 gtaagcattt taagtgcgat atcttttatg aataagttcc ggtctctccg ggatgataga
    12481 aagaacaaca taaaacaccg ggcgctattc taggttttga tgaacggaac gaatgtatta
    12541 ccgatgcgac ctggtggccg caccgtcttc cggattattt atttacaacc ctcatcgacc
    12601 tcggtctgct gaagtccagg tactgcccgt tccaagcttt aacttttttc atatcagctg
    12661 gattcacatt acccacaaac tcaccaatcc tgttatagga caggttatcc cagtcagaga
    12721 gcagcacttt cgctttggcc ccgctgtcga catagacttc ctggcctgcg atccagtctg
    12781 gcagactgga tcctgaccgc tggccaacgg ggagacagta acgctgggca gtcttcacag
    12841 aaacgataca tccctcgttt tcttttgccg gaagtgcccg accatttaca gtataagtca
    12901 gaccttctgg tgctggtgtg agtgttcggg tatccaatac tttgttatta cagactaaaa
    12961 tcgcctgagt gggctgactt tcagtcggca cgttgatatg gaacttgttc atcactgaac
    13021 tgttcacacg attattagcc agtttgaatc gtagctgccc ttctctagta tccacctgca
    13081 gctggcagtc attactggac aggttctcac tgtcatcaga ataagtgaaa ccatacgcac
    13141 cgtacattgc aggatagatg aagctagaca aagtgccttt gggatcgtaa taacccacca
    13201 gtgtcgtcac cggaacgcca aacttctctg gcttacgcgc ttcgcgggta ttcaccacgt
    13261 cactttcttt ccaggtctgc ccatcggaaa caaagctctt cttatatccc ttggaaatta
    13321 tcttttcacc accgttgata aacagatggc tgttataacc agcttcatga tcgagtgtca
    13381 gaatacggcc tttattctcc gcggaagctg caggggcatg aatatctcta gtccagttgc
    13441 cgtccgacat atgtactttg acaataggat attctgccat catctcagcc attttgtttt
    13501 cactgagatc cctgacagaa gcagtaatct gctcaaacct gtcaatggtg tgttcatacg
    13561 gttccatttt atgctcatcg gcattccact tgctgaatcc agtggaggaa aggctatcga
    13621 acacagcttt attttcaaaa aagcgctgga tgatagccga tgaatttggc gtatacattg
    13681 tgaaacggtt tgcgtcggag aaaggactgc ctcccgccat ggcgtcaaag ccaaacgagt
    13741 gaccgtcgaa cggctcctgg cactgctcgt caagacaact tttccggttt gtctgggctg
    13801 gataaaaatt aggtatgaaa cgttttttat cgctgtccca gccccaagtc gagttgatct
    13861 ggtcagcgct gcgatgcacg gaccccctga agccacccac ataatgacca agaccgaagt
    13921 tatgcccgac ttcatggctg aattcattac ccaaggtaga gtctagggta acaattcccc
    13981 ctccaccgga accaccgtgc acctgtactc cattggagta gttaccgcgg tttgtatgtg
    14041 cggtcagttg cgcagccaca taaggatggc tgctttctcc tgaaccagca gtgctgttaa
    14101 taccatagtt ggcattatca ataccatgag agatcagctc cttaccgata ctctgtcgca
    14161 tcgtgccgct atgccatcca ccctcaccgg gatctgcatc tgtcagcaat gtaccggtag
    14221 gtagcatgac ttccttcaga tgaagtggag cataattgtt gacgatcata cgactgaccg
    14281 ggatcgtctg gaaatattcc cgatgcgcct ctttgtcctt ggcaaaatca aagcgccccc
    14341 ggggagtggt cagcatcccg atatcgatgg tatgcaacaa cagctcccct ggtgctccaa
    14401 cattgatgtc atttaggcta ccactcaggt taccttgttt aatcaccaag tttaacccgg
    14461 gcacgatcca gtgcgcaggc agttcagcac tccaggtatg ctgagcataa gcgattcggt
    14521 tattctccag ctcaccggag cggaaccact gaccatttac atatttgaac agcagggtgt
    14581 tacccaccga gagcgtgacc tttcgatctc cataaaaaac cgttgaatta tagccggcat
    14641 acgaaacaaa gcgaaccatc ttaccttcaa gctcggctcc ctgcggcaaa tacatgtctc
    14701 ttatccaacg accatttgct gtctggatct ccaccagtgc attatttgcc agatagcttt
    14761 taatagaact cccgctggca tcactgagtt tgtttacttc agcaaccgta ttaatgatct
    14821 ttttcgttcc attctgagga gtaaaatcga taccatcagc aggaacacca tctaggtggt
    14881 agacggtatc cggtagtgag gagggaggcg aaagggttaa cgtaccgaga attttatcat
    14941 tagcatctcg ggcttccacc tgaacaggtg ttttatcatc agccttcacc agacggacca
    15001 acagcaaact tttccgcagg ctggtcagat gttgttgact ttccccttct ttaggatggg
    15061 cagataaaat ctggctctgt gcaaatttca cctcagcggc cagtcccccc tgcagatcat
    15121 ttacgggctg ggtggtattg aaataaatgg ctgaattatt atcagcagca gttgcagcaa
    15181 atgctcccat cgccagagag gcaaggatca tgcatgacag atactttaat ttcataagtg
    15241 tactccatct ttcattttag atttcatgca gccggatata atagaagtgt taaattttat
    15301 tttttcagga gtgattcact cattcaccag tgttgatttt tataacacac ttatatctac
    15361 ttcagaaaga taacctgata aaaaatatta actccgatca cattcttcta tatattccat
    15421 cacaatattt tgtttaaaaa atgttaaatt cactatcttc gtgataatta acaaacaggg
    15481 tacaaaacca gtaactacaa aatacaataa aaaaaataca tatcgcattt tatcgaacat
    15541 agtgaacatt agctattgct atattttatt tttttgtatt aatctcattt aaaaaaacaa
    15601 ccacaacgcg aaaacaataa ataaataaca aataaaactc atttttatta aaataaaaaa
    15661 caaaataata catccttaat cacaatatcc agatgctcct ccccacgtaa tatagacaca
    15721 ggcccaagcg aggttctggt tttcaaattg ttccggacag agaccgccac accaactgtg
    15781 ccgtcgtcac cgattgtaat cacattcgat ca
//
LOCUS       DAZFYC010000114        15682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFYC010000114 DAZFYC010000000
VERSION     DAZFYC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.114"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..760)
                     /locus_tag="ACHKW5_003963"
     CDS             complement(<1..760)
                     /locus_tag="ACHKW5_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO7513254.1"
                     /translation="MISHIPNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVPGLNRDN
                     FIYISNIFLNIKQRNEKNHTINMFREVSISNDTISVKFYRNEKIECACNFLMDKDAQG
                     YIDLSDMNLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGYCNFTTATVDNVIFRCRR
                     LHNVIFIKASGECVDFSKNILDTVDFSRSQLTHSNFRECQIRNSNFDHCYLYASHFTR
                     AEFLSDKEISFIKSNLTAVMFDHVRISTGNFKDCI"
     gene            complement(989..1909)
                     /gene="malM"
                     /locus_tag="ACHKW5_003964"
     CDS             complement(989..1909)
                     /gene="malM"
                     /locus_tag="ACHKW5_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO7513255.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAASAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(2152..3492)
                     /gene="lamB"
                     /locus_tag="ACHKW5_003965"
     CDS             complement(2152..3492)
                     /gene="lamB"
                     /locus_tag="ACHKW5_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO7513256.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAVTRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAYVDEKFSYDINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTLMEIGYDNVKSQRTGDTNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTDKAKGTPNYGKAVPANFEGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3564..4679)
                     /gene="malK"
                     /locus_tag="ACHKW5_003966"
     CDS             complement(3564..4679)
                     /gene="malK"
                     /locus_tag="ACHKW5_003966"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO7513257.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5044..6234
                     /gene="malE"
                     /locus_tag="ACHKW5_003967"
     CDS             5044..6234
                     /gene="malE"
                     /locus_tag="ACHKW5_003967"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO7513258.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6388..7932
                     /gene="malF"
                     /locus_tag="ACHKW5_003968"
     CDS             6388..7932
                     /gene="malF"
                     /locus_tag="ACHKW5_003968"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO7513259.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7947..8837
                     /gene="malG"
                     /locus_tag="ACHKW5_003969"
     CDS             7947..8837
                     /gene="malG"
                     /locus_tag="ACHKW5_003969"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO7513260.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            9209..10684
                     /gene="xylE"
                     /locus_tag="ACHKW5_003970"
     CDS             9209..10684
                     /gene="xylE"
                     /locus_tag="ACHKW5_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO7513261.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLNESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10728..11138)
                     /gene="psiE"
                     /locus_tag="ACHKW5_003971"
     CDS             complement(10728..11138)
                     /gene="psiE"
                     /locus_tag="ACHKW5_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO7513262.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11264..11392)
                     /locus_tag="ACHKW5_003972"
     CDS             complement(11264..11392)
                     /locus_tag="ACHKW5_003972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513263.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            11408..11686
                     /locus_tag="ACHKW5_003973"
     CDS             11408..11686
                     /locus_tag="ACHKW5_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513264.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11733..13829)
                     /locus_tag="ACHKW5_003974"
     CDS             complement(11733..13829)
                     /locus_tag="ACHKW5_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7513265.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13829..14566)
                     /locus_tag="ACHKW5_003975"
     CDS             complement(13829..14566)
                     /locus_tag="ACHKW5_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7513266.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAASRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14563..15201)
                     /locus_tag="ACHKW5_003976"
     CDS             complement(14563..15201)
                     /locus_tag="ACHKW5_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO7513267.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15315..15557)
                     /gene="yjbE"
                     /locus_tag="ACHKW5_003977"
     CDS             complement(15315..15557)
                     /gene="yjbE"
                     /locus_tag="ACHKW5_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO7513268.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT         3726 a         4062 c         3950 g         3944 t
ORIGIN      
        1 taatgcaatc tttaaaattc cctgtcgata ttcgcacatg atcaaacata acagctgtca
       61 aattcgattt gataaatgat atttctttgt cagaaagaaa ttctgctctg gtgaagtgcg
      121 aagcataaag ataacaatga tcgaagtttg aatttctaat ctgacattcg cgaaaattac
      181 tatgagtaag ctggctccgc gagaagtcaa ctgtatcaag aatatttttg ctaaaatcga
      241 cacattcacc actcgctttg ataaaaatca cattgtgtaa acgtcgacat ctaaatataa
      301 cattatcaac tgttgcagta gtaaaattgc aatacccaat ttctttacat tcgaatgtta
      361 catgttgtag atttgatgat aaaaaagaca ccttcgatat aacgtcacct ttaaaatgac
      421 aacttgttaa attcatatca gacaggtcga tatacccctg cgcatcttta tccatcagaa
      481 aattacaagc gcattctatt ttttcatttc tgtagaattt tacacttata gtatcattac
      541 taattgagac ttcacgaaac atatttattg tatgattttt ttcgttccgt tgttttatat
      601 ttaaaaaaat attgctgata taaataaaat tatctctatt taaacctggc accccattaa
      661 aattacgtag taaatcaaga tctactatat tatttccttg caccacatca tgtttcatca
      721 aagagttatt gccgccagaa tttgggatat gacttatcat aaaacctctg cataacagaa
      781 ataaaataac ctggaaaagt agcctgaatg tccagacact accggcgaaa ggaaaagaat
      841 ctctgataag gcattgagat aatggacatt cttagtgaag aagaagtgag ttactataaa
      901 aaatcgctca tatcccaaga aggaagcata aaaaaaggtg cgccaggaga cgcaccagtt
      961 gttgcaaaat cagcactgtg gggcgtaatt accccttgcc ttttacactg ctgataaagg
     1021 tggaacgggc agatgtcgat cccaggcgtt cagcttcatc aagcagtttt aacgccttat
     1081 caacatcacc tttcgcgaca gcgtttttga tcgcggtatt aaaataactt tccgtgtcgt
     1141 tgagcatcgg ctcgcttttc ttcaccggtg ccggagcgga tgcagccaca gctggtgccg
     1201 ccgtgttgcc taccgtaacc ggagctggag cggaagaacc aaatagtggt cctaccaaca
     1261 cgctggagct ggagttcgtt ttgactttca gtttcagtaa gccatcgctg gtatgacgag
     1321 caaccggatc gggaatatcc gggatcgagt tacccacgcc tttggcgtag gctttagccg
     1381 ggtcgagcag ttgggtcgtc tgctggagat ctttttccgt ggtaaagacc agaacataaa
     1441 gtttttgctg ccccaacgcc ggtgtcaggc gcataacgcc ttccagccga tctgcactca
     1501 tcacgcctgg ttcctggtag gtgaaataac tgctggggaa gaaggctgat ggggtcatgt
     1561 tctgatcaag aatcagcacg ttcggcgcaa atacgctggt ttgtttgttc acttcgctgg
     1621 tcagcgtcag ggtcagttcg ccaatgtttg ccgggacgct gtacgcagca accggaccac
     1681 tgatgccggg aacgttcagt tgttggccgc cggtcgccag ttgggtggtc tgggttttag
     1741 attgatcgac tggtgtccag gtgagttgtt gcagcgcggc agatggaatg gctggcgcgt
     1801 cgctggtgtt ttgcggtacg tagttaacat cggcaaggct gattccaggc gcgcttgcca
     1861 gtaaccctgc tgataaacag agggcgatga gacttttatt cattttcatt gttttcacct
     1921 caaaatctgg agctcagcgg tagccaggca atagcgcgct aaacccgata atcagagggg
     1981 cttgcgcccc tttcagataa tgacaacctg tttttatgcc ggatgcggcg taaacgcctt
     2041 atccggccta catttgacag ccgttgtagg cctgataaga cgcgcaagcg tcgcatcagg
     2101 cgttggatgc cggatgcggc gcaagcacct tatccggccc aggttttgct attaccacca
     2161 gatttccatc tgggcaccga aggtccactc gtcgctgtcg ccacgaccga agctgccgcc
     2221 ttcgaagtta gctggaacgg ctttgccgta gttcggagta cctttagctt tgtcggtgta
     2281 atcgtaaccc catttctcat cccacttggc gtaggtggca aaaacacgaa tagccggacg
     2341 cgaccagatg ctgtcgccag cctgccattg ttgagcaagt gtgattttgt actggttgtt
     2401 ggtatcacca gtacgttgag atttaacgtt gtcgtagccg atttccatca gggtactcat
     2461 gatcggcgtc cacttataca tcgggcgaat accgacggtc caccacttgg tgccgttgtc
     2521 gttatcccag ttgatatcct ggtacatacc cacgtacatc atgtcccagt tgtcgcccat
     2581 ggagatcgca ccgtggtcga ggatacgcag catgtgaccg ttgttgttaa tgtcatagga
     2641 aaatttttcg tcaacatatg caacaccaga accctgcgac agacctttac cctgcgaggt
     2701 catcgagtca gtagcgtact gaacaacaaa cttgttaaag cccttcagga cactctgagt
     2761 atgttcagca gtgaataacc agccgtcttt cgatgcgcca tcaaccagac gatagttatc
     2821 acgcaggttg gcacgaccgt agtcgacacc cagttctaat gtgccgcccg ggttgatttc
     2881 catctgcgct aaacgcacat cgaaaacgtc gttcgcggtt tcgttggtat agtcataaat
     2941 attgttgctg gcgaaagagg aagaaccacc tgcttcagag gagcgggtta ctgccagaga
     3001 gagtttaccg aagccaacat cgatgttttc cagaccggca ccaggaccag aaatatccca
     3061 gtagtagaag tcgatcatat gaacgtcatg acgttggtag aagcgcttac cagcccagat
     3121 ggtggagcct ggcagccatt cgatcagatt tttaccctgc acgtttgctt cacggaaggc
     3181 cggatcggta gcttcccagt cattttgttg tgcgacggaa taggccacat tagtgtcgaa
     3241 atagaagctc ttatcgccct ctttccacac ttcctgaccc aattttaatt cagcataagt
     3301 ttcacattcg ttgccaagac ggtatttact ttgagcaccg gtagtctgga aacactgttg
     3361 ttcaccgccg ctacctgtcc agccaatacc ggaacgtgca tagccgtgga aatcaacagc
     3421 cattgcctga gcagacatta cgcccgctgc gacggcaacc gccagaggaa gtttgcgcag
     3481 agtaatcatc attctatctc ctgagtcatt gcttttcttt tttcacatca cctgtgacag
     3541 gctttgtgtg ttttgtgggg tgcttaaacg cccggctcct tatgcagtcg acgacatgca
     3601 gtgccatcct cacggaacag atggcaacgc tctggcggca ggccgatagc gaatgtggca
     3661 ccttcttcta ccaacaccac gtcgttctgg cggtacacca ggttttgacg aatggaaggg
     3721 atctggatat ggatttgggt ttcgttgccg agttgctcga cgacctgaac ttcaccctca
     3781 aggatgacgt cagcgatatc actcggcagt agatgttccg ggcgaatacc cagcgacata
     3841 ttggctccaa cctggacatc acggctttca actggcagcc agacttgctg acgatttggc
     3901 atcggcagct ccacctgcac ttgatcgatt gcggtggcgg tcacttttac cggcaggaag
     3961 ttcatctttg gcgaaccgat aaatccggcg acaaaacggt ctgccggata gtggtacagc
     4021 tccagcggtt tcccaacctg cgccacgcga ccggcgtcca gcaccacgat tttgtcggcc
     4081 agcgtcatcg cttcgacctg atcgtgggtg acgtaaatca ttgtgcggcc caggcgttta
     4141 tgcagacggg agatttcgat acgcatttgc acacgcagtg cagcatcgag gttggagagc
     4201 ggttcatcga gcaaaaatac gcttggctcg gccaccagcg tacggccaat cgccacacgc
     4261 tgacgctgac cgccagagag cgctttcggt ttgcgatcca gcaaatgcgc cagttgcagc
     4321 acttccgcca cctggttaac gcgttggtta atcacctctt tttttgcgcc agccagtttc
     4381 aggccaaatg acatgttttc tgctactgac aggtggggat agagcgcgta agactgaaac
     4441 accataccaa cgccgcgttc tgctggcggt gtgtcattca tccgtttctc accgatgaac
     4501 aggtcgccgc tggtgatcgt ctcaagcccg gcaatcatgc gcagtaaagt cgatttaccg
     4561 cagccagacg gtccgacaaa caccacgaat tcaccttcat ggatatcgag attgatatct
     4621 ttcgatacca cgacctcgcc ccaggctttc gttacatttt gcagctgtac gctcgccatg
     4681 cccttctccc tttgtaacaa cctgtcatcg acagcaacat tcatgatggg ctgactatgc
     4741 gtcatcagga gatggcttaa atcctccacc ccctggcttt tttatggggg aggaggcggg
     4801 aggatgagaa cacggcttct gtgaactaaa ccgaggtcat gtaaggaatt tcgtgatgtt
     4861 gcttgcaaaa atcgtggcga ttttatgtgc gcatctccac attaccgcca attctgtaac
     4921 agagatcaca caaagcaacg gtggggcgta ggggcaagga ggatggaaag aggttgccgt
     4981 ataaagaaac tagagtccgt ttaggtgttt tcacgagcac ttcaccaaca aggaccatag
     5041 attatgaaaa taaaaacagg tgcacgcatc ctcgcattat ccgcattaac gacgatgatg
     5101 ttttccgcct cggctctcgc caaaatcgaa gaaggtaaac tggtaatctg gattaacggc
     5161 gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt
     5221 aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tccctcaggt tgcggcaact
     5281 ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct
     5341 ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc
     5401 tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta
     5461 tcgctgattt ataacaaaga tctgctgccg aacccaccaa aaacctggga agagatcccg
     5521 gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa
     5581 ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac
     5641 ggcaagtacg atattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc
     5701 ttcctggttg accttattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca
     5761 gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc
     5821 aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa
     5881 ccatctaaac cgttcgttgg cgtgctgagc gccggtatta acgccgccag tccgaacaaa
     5941 gagctggcga aagagttcct cgaaaactat ctgctgactg acgaaggtct ggaagcggtc
     6001 aataaagaca aaccgctggg tgcagtggcg ctgaagtctt acgaggaaga gttggcgaaa
     6061 gatccacgta ttgccgccac catggaaaac gcccagaaag gtgaaatcat gccgaacatc
     6121 ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt
     6181 cgtcagactg tcgatgaagc cctgaaagac gcgcagactc gtatcaccaa gtaatgctgt
     6241 gaaatgccgg atgcggcgtg aacgccttgt ccggcctaca aaactgaaac gcatgtatgc
     6301 ctgataagac gcatcagtgt cgcatcaggc agttgttgtc ggataaggcg tgaaagcctt
     6361 atccgtcctg gaatgaggaa gaaccccatg gatgtcatta aaaagaaaca ttggtggcaa
     6421 agcgacgcgc tgaaatggtc agtgctaggt ctgctcggcc tgctggtggg ttaccttgtt
     6481 gttttaatgt acgcacaagg ggaatacctg ttcgccatta ccacgctgat attgagttca
     6541 gcggggctgt atattttcgc caatcgtaaa gcctacgcct ggcgctatgt ttacccggga
     6601 atggctggaa tgggattatt cgtcctcttc cctctggtct gcaccatcgc cattgccttc
     6661 accaactaca gcagcactaa ccagctgact tttgaacgtg cgcaggaagt gttgttagat
     6721 cgctcctggc aagcaggcaa aacctataac tttggtcttt acccggcggg cgatgagtgg
     6781 caactggcgc tcagcgacgg cgaaaccggc aaaaattacc tctccgacgc gtttaaattt
     6841 ggcggcgagc aaaaactgca actgaaagaa acgaccgccc agcccgaagg cgaacgcgcg
     6901 aatctgcgcg tgattaccca gaatcgtcag gcgctgagtg acattaccgc cattctgccg
     6961 gatggcaaca aagtgatgat gagctccctg cgccagtttt ctggcacgca gccgctctac
     7021 acactcgacg gtgacggcac gttgacgaat aatcagagcg gcgtgaaata tcgtccgaat
     7081 aaccaaattg gcttttacca gtccattacc gccgacggca actggggtga tgaaaagcta
     7141 agccccggtt acaccgtgac caccggctgg aaaaacttta cccgcgtctt taccgacgaa
     7201 ggcattcaga aaccgttcct cgccattttc gtctggaccg tggtgttctc gctgatcact
     7261 gtctttttaa cggtggcggt cggcatggtt ctggcatgtc tggtgcagtg ggaagcgttg
     7321 cgcggcaaag cggtctatcg cgtcctgctg attctgccct acgcggtgcc atcgttcatt
     7381 tcaatcttga ttttcaaagg attgtttaac cagagcttcg gtgaaatcaa catgatgttg
     7441 agcgcgctgt ttggcgtgaa gcccgcctgg ttcagcgatc cgaccaccgc ccgcacgatg
     7501 ctgattatcg tcaatacctg gctgggttat ccgtacatga tgatcctctg catgggcttg
     7561 ctgaaagcga ttccggacga tttgtatgaa gcctcagcaa tggatggcgc aggtccgttc
     7621 cagaacttct ttaagattac gctgccgctg ctgattaaac cgctgacgcc gctgatgatc
     7681 gccagcttcg cctttaactt taacaacttc gtgctgattc agctgttaac caacggcggc
     7741 ccggatcgtc ttggcacgac cacgccagcc ggttataccg acctgcttgt taactacacc
     7801 taccgtatcg cttttgaagg cggcgggggt caggacttcg gtctggcggc agcaattgcc
     7861 acgctgatct tcctgctggt gggtgcgctg gcgatagtga acctgaaagc cacgcgaatg
     7921 aagtttgatt aagggagata acaaaaatgg caatggtcca accgaaatcg caaaaagcac
     7981 gtttgtttat tactcacctg ctactgctac tttttatcgc ggcgattatg ttcccgctgc
     8041 tgatggtcgt cgctatctcg ctgcgtcagg gaaacttcgc gaccggtagc ctgatcccgg
     8101 agcaaatctc ctgggatcac tggaaactgg cgttaggttt tagcgttgaa caggctgatg
     8161 gtcgcattac gccaccgcca ttcccggtac tgctgtggct gtggaactcg gtaaaggtcg
     8221 ccgggatttc cgcgattggc attgtggcgc tctccaccac ctgcgcctac gctttcgccc
     8281 gtatgcgctt tccaggcaaa gcgacgctgc tgaaaggaat gctgattttc cagatgttcc
     8341 cggcagtact ttcactggtc gcgttgtatg cgttgtttga tcgtctgggt gagtacattc
     8401 cattcattgg tctgaatact catggcggcg taattttcgc gtatctgggt gggattgcgc
     8461 tgcatgtctg gaccatcaaa ggctatttcg aaaccatcga cagttcgctg gaagaagcgg
     8521 cggcgctgga tggtgcgaca ccgtggcagg ccttccgcct tgtcctgttg ccgctgtcag
     8581 taccgattct ggcggtggtg ttcatcctgt cgtttatcgc tgccattact gaagttccgg
     8641 tcgcgtcgct gttactgcgt gacgtaaata gctacaccct ggccgtgggg atgcagcaat
     8701 acctcaaccc gcaaaactac ctgtggggtg actttgccgc cgctgccgtg atgtctgcat
     8761 taccgatcac catcgtcttc ttgctggctc aacgctggct ggtcaacggc ctgacggcag
     8821 gtggtgtgaa aggttaaaga tgttgttctg ccaatgttat gccgctgcac cctcaactta
     8881 cgttatccca acttgtgact attattcggc gctccacgga gcgccttttt ttctttcgtc
     8941 tgcaatctga atcgttcgcc ggttaatatt cccatcatag aacttattat ttttacgtta
     9001 tttgttttcc cacttacgat aattctcttt cgtgctctga atcacggcaa tagtattgtt
     9061 tttatcaatt ttggataatt atcacaatta agatcacaga aaagacatta cgtaaacgca
     9121 ttgtaaaaaa tgataattgc cttaactgcc tgacaattcc aacatcaatg cactgataaa
     9181 agatcagaat gatctaaggc aggtctgaat gaatacccag tataattcca gttatatatt
     9241 ttcgattacc ttagtcgcta cattaggtgg tttattattt ggctacgaca ccgccgttat
     9301 ttccggtact gttgagtcac tcaataccgt ctttgttgct ccacaaaact taaatgaatc
     9361 cgctgccaac tccctgttag ggttttgcgt agccagcgct ctgattggtt gcatcatcgg
     9421 cggtgccctc ggtggttatt gcagtaaccg cttcggtcgt cgtgattcac ttaagattgc
     9481 tgctgtcctg ttttttattt ctggtgtagg ttctgcctgg ccagaacttg gttttacctc
     9541 tataaacccg gacaacacag tgcctgttta tctggcaggt tatgtcccgg aatttgttat
     9601 ttatcgcatt attggcggta ttggcgttgg tttagcctca atgctttcac caatgtatat
     9661 tgcggaactg gctccagctc atattcgcgg gaaactggtt tcatttaacc agtttgcgat
     9721 tattttcggg caacttttag tttactgcgt aaactatttt attgcccgtt ccggtgatgc
     9781 cagctggctg aatactgacg gctggcgtta tatgtttgcc tcggaatgta tccctgcact
     9841 gctgttctta atgctgctgt ataccgtgcc agaaagtcct cgctggctga tgtcacgcgg
     9901 caagcaagaa caggcggaaa gtatcctgcg caaaattatg ggcaacacgc ttgcaactca
     9961 ggcagtacag gaaattaaac actccctgga tcatggccgc aaaaccggtg gtcgtctgct
    10021 gatgtttggc gtgggcgtga ttgtaatcgg cgttatgctc tccatcttcc agcaatttgt
    10081 cggcatcaat gtggtgttgt actacgcgcc ggaagtgttc aaaacgctgg gggccagcac
    10141 ggatatcgcg ctgttgcaga ccattattgt cggagttatc aacctcacct tcaccgttct
    10201 ggcaattatg acggtggata aatttggtcg taagccactg caaattatcg gcgcgctcgg
    10261 aatggcaatc ggtatgttta gcctcggtac cgcgttttac actcaggcac cgggtattgt
    10321 ggcgctactg tcgatgctgt tctatgttgc cgcctttgcc atgtcctggg gtccggtatg
    10381 ctgggttctg ctgtcggaaa tcttcccgaa tgccattcgt ggtaaagcgc tggcaatcgc
    10441 ggtggcggcc cagtggctgg cgaactactt cgtctcctgg accttcccga tgatggacaa
    10501 aaactcctgg ctggtggccc atttccacaa cggtttctcc tactggattt acgggtgtat
    10561 gggcgttctg gcagcactat ttatgtggaa atttgtcccg gaaaccaaag gtaaaaccct
    10621 tgaggagctg gaagccctct gggaaccgga aacgaagaaa acacaacaaa ctgctacgct
    10681 gtaatcttcc tgtccagcac gccgcgccag ttcggcgtgc tgtcttttta ctcccgcttc
    10741 agccgtttcg aattacacaa ccacagagtg atcaccagta acaggatcgc agccgagtag
    10801 atcaacacat ccagtggcga tttatgatcg acgatgatca agcgcacaat ggcggtgatc
    10861 ccaatataga caaagtaacg tagcgggaag tgaaaaccgg actgaaagta tttcacaatc
    10921 agcgcgataa attcgaaata gagaaagtaa accaccagtc cttctaccag ctcatatttg
    10981 ctggtttgtt ctggcgcgaa cagcacatct gccagatgca ccgtttcttt gccgaggaag
    11041 acaaccaaaa tcaggccgag gcacaacagg ccaagattga gtacggtctg caaaatggtg
    11101 gagataaact ccacgcgcgg acgagagagt gacgtcatag cagtacctcc ttcacagtgg
    11161 cgagcttcct gtataacgca aaaatgtgac ggagatctat attttttgtg tatgttttgt
    11221 tcaacctgga tttggagcgc gcctccacca aagccggata tacctacccg taggccggat
    11281 aagccgcgcc tggcggcgca tccggcaaga caaaccgcac caaagccggt aattcaaaaa
    11341 tcacccattc aggtgattcc cagccaccag caatgcgcga aaaatcgacc atgttcctct
    11401 ggaacaaatg aaacgtaacc tgataaaggt ggtaaaaatg aagccttatt ttgctgcttt
    11461 gatgttatca gtctctgttc ttcctgctta tgctggtccg ctcggtactg ctgataaagc
    11521 cgacctaccg caaagcaatg tctccagccc aatgatggcc cagtcgctca ggcaaccaga
    11581 tctgcaaccg atatcgacgg acaggaagac agaatgtttt cgcctttaca caccagatcg
    11641 caaaccgggg gtgaactgcg ttcctgatgg ctcaacaggc cattaagaat gagggtgcca
    11701 gtgattgcga aaactggcac ctttcccatg acttagcgga aattgacgct acggtcgctg
    11761 gtcatgtcat acaactggaa cttacgccca agttgctgac caccgtcacg cgtcagcggc
    11821 gtccagccaa tcgccgcacg gctgcgtgtc gggccagacg agaagagatc caacggtacc
    11881 gagacataca cgcctttggt gaagtcccct tcgccgtact cctcttttga aacattagtg
    11941 atcgtggcat agccacccac caccacgccg ctatcaaagc gtttggcgat ctccagcgtg
    12001 ccgcctttat cccctgccag atactgcccg acgctggctt taactaacac atcctgagcg
    12061 aaagatggcg tccagtaggc ggtcagatgt ccggttttca cgctgtagtc ggtgaatttc
    12121 atcatatctt ttgcactacg ccagtcgcgc tgtttaacgt agttggcatc cagaccaaac
    12181 gcccagttgc tgtccagcgg gcgatacaac acttctgccc ccgcaccgcc aaacatggtt
    12241 tcgagataac caccgtagac ctgaccgtag aagccgttgc ccagatgctg gaagtagttg
    12301 gcttgcaggt tattcacata gacatcgttc tgcacatact cgcgcacatg ggtacgaacg
    12361 cgcggtaagt gcgagtcctg cggtggattg gtgtagttaa acttgtcgta gttgttggca
    12421 agatttgcaa acaggctgcc ggtggtcagt agatggtccg tcagccacaa atctgccgtt
    12481 cccatcacgc ccagctgata catgtaaaag ttttccgggc caccgaccga ctggttcagc
    12541 accggatcga tatggaaatc aaagcgtgat ttgtcgatat accagccctg ctcggtggac
    12601 tgcggaacca ctggctcgac gcgtttttgc gccagcgtcg tttcgtggcc caacggctct
    12661 ccggcgagat gatttttcag gctggcgaca tcggtttccg tcgtcacctg cggcatgtta
    12721 aggcgatttt ccgtaacgcg gatcgttttg atcccatccg gcagatcatt catcacgatc
    12781 cgattagcac ggatgatccc ttcgcgcgaa tcacgatatt tcacctgctc gccggtaaca
    12841 tacagcgtat cgcctttcgc ctggatctgc ggatcggcaa gtccggcatt gtatttcaac
    12901 agcgttaact gattcgccac caccgaatgc tgcaaaatgg catcctgcgg ctgcggttga
    12961 tattgcgggc gggcgttatc gatgtaggac gggcgcagat cgttaaagtt ggtgcgcagc
    13021 gtgacgccaa acataaaggt gttgccacgt tcatagctaa ggttaacgtc ggcccaatcg
    13081 gtaacgcgat aaatcgcacc gacgttaaac ttactttttt gctccagctt cccggcaaaa
    13141 tcctgctgat aattattgcc ttcatactcc agtttcaggc gcagcggttg ccagggcgtc
    13201 tggtattcca cgccgccaaa cagtgaggca ggaccgtgga acatctggct accgtcgata
    13261 gatcccgcct gtttgtagct gttatcgcga tagcaatatt tatcactggc tgaacagagc
    13321 ggatttttca cattaccgct ggtgcccaga tatccccagc ccaggccgag cgtaaaatcg
    13381 aacggccccc aggctttgct ggcaacaaga tattccgcat caaacagccc cgtaccgccg
    13441 atatcccgcg caccaaccgc cacttgcggc agccagtaac tctcttccca caaacgcagt
    13501 ttgagatcga aggctttatc tttatacgtt tgatcgccag agaacgcttc gacgctgctg
    13561 tactgccggg tgcgcacgtc ggtgtagcgc agcgttgttt ccagccacgg gaagagttgc
    13621 actgaagctg agtaataacg gtactgatcg ttatctcgat agttcaggct caactcccct
    13681 tcccgcgcca tgcgcgcggt gggcgtttgt aataatccta cgccaccgaa atccgactgc
    13741 gacggaccaa tgggcgcagg atatgtttcg ccgtagcaag ctgtgctaat gcccagcgcc
    13801 agtaagctaa gcagatgtct ttttttcatt attggggtat ccgctgcgtc agagtctgaa
    13861 gaatgtcggc gttaagggca tcaggcgtct cactccagac ggagtccgcg aggccaacat
    13921 aaataatgct gccgggcatc ggctctacgt gacgcttgtt ccagtaagcc accggcgctt
    13981 tttgcgtgcg tccgtccggg taaaccaccc acgcgtagct gcgatccgca ccgctaagca
    14041 ggttctgccc ggagagatag ctcgccacgt cgcgacctgg agtgaatggc tgcttgccag
    14101 gacggctgat aagcccgaac agcgtgaccg tggacggtgg tggcccgacc cacagcgtgt
    14161 agttgccctg caacggtggg ttaccgcgtt cggcaacgcg gacgatatca ggatcaagat
    14221 tgattttttg cctgcccgtc actttcaacg cctgaatttg ctggcgtaag gcgttaatcg
    14281 cggcggcatc gtcggcgctg gaatctgcag ccagttctgc cagccgtgtc agcaacgcct
    14341 gctgctgccg cgatgctgcc gccgttgcca gctcttcgct aatcaccgca ccaggccacc
    14401 aactgtttgc cagccgcggc tggcctacca gatcgattaa atactctgcg cccgtcagcg
    14461 ttttggcctc actgctgcca ttgctgaata ccttgacggt tcccgccgcg aagaccgatg
    14521 acgcccccac actcaaaatc aatgcgacaa tagtttgttt aatcattgtt ttgccgcctt
    14581 gatgagagtg gttttcaccg ggaaataatc cgcgccgaga tactgttccg actggcgaat
    14641 ttgcccttcg ctgtcgatcc agtagcggtt atgccagcgc gcctggtcgg tggagacttc
    14701 ttcgtccagc acgcgaaccg gggtttcatc gctgccgacc ttgacggtat cggtgccatc
    14761 ccatttgaag actgagcgtg cggtggcgta gcgtacctgc tggtactcgg tccagcccat
    14821 cgtgcgcgtc catgttgcac catcaacaat ttgtgcgggt ttaatcagcg ggtcggcggc
    14881 aaggttattc acttcaatca ggttgtcgcc gccaagcaaa gtcttcacca gacgaccatg
    14941 ttgtgtgaca agagtggctt gatcctgagt gacccatttt tgttgtccgt cttcagcgaa
    15001 ggccagcacc acaaacaatt gcggcccgcc attaagctgc atgtactggc tggcgtaggg
    15061 catattttga atatcatcgt ccgtcagctg tacgcctggc gtgccgaaca gactgtccca
    15121 cagtgaattg cccagctctt tagtcgtggc tgaacaggcc tgtaatagca ggcagataag
    15181 aatgagtgca ggtcgcttca cgactcttct ccagaggggc agaataacca caccggagtg
    15241 tggttatggt aaatcccttc atacttcaag ccacagatgc aatgactcaa attattttgg
    15301 ggatacatac tttattactg ggtactggtg gtcgtggttg tggtggtccc ggtattagaa
    15361 ccatcaccgc caccggttgc cgcgagcgcg acacccaccg ccgagcttac ggtgctgacg
    15421 ctggtcgcgg aggaactccc cgccgaaacc gacgttgctg ccgaccctgc cgcttcgccc
    15481 acctgtacag gtgcagccca cgcagaagtc gccgcaagcg cagatatggc aaaaatgcca
    15541 tacagaactt ttttcataac aatttccctt cattgaatga atggagattt acccaaaaac
    15601 atttcgggcg ggatcgagta tacacaacta aagcatgcga attacggcgc ggggaattag
    15661 agatggggtt ttaggataat tg
//
LOCUS       DAZFYC010000115        15662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFYC010000115 DAZFYC010000000
VERSION     DAZFYC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.115"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..407
                     /gene="espX1"
                     /locus_tag="ACHKW5_003978"
     CDS             <1..407
                     /gene="espX1"
                     /locus_tag="ACHKW5_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO7513269.1"
                     /translation="LSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCVDIMPGRW
                     TTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKF
                     MHDYDKTSSEMRKYLKSINECMFLMKNIAHQN"
     gene            654..1985
                     /locus_tag="ACHKW5_003979"
                     /pseudo
     CDS             654..1985
                     /locus_tag="ACHKW5_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1998..2956
                     /locus_tag="ACHKW5_003980"
                     /pseudo
     CDS             1998..2956
                     /locus_tag="ACHKW5_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(2995..3894)
                     /gene="nhaR"
                     /locus_tag="ACHKW5_003981"
     CDS             complement(2995..3894)
                     /gene="nhaR"
                     /locus_tag="ACHKW5_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO7513270.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3960..5126)
                     /gene="nhaA"
                     /locus_tag="ACHKW5_003982"
     CDS             complement(3960..5126)
                     /gene="nhaA"
                     /locus_tag="ACHKW5_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO7513271.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5712..5864
                     /gene="mokC"
                     /locus_tag="ACHKW5_003983"
     CDS             5712..5864
                     /gene="mokC"
                     /locus_tag="ACHKW5_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO7513272.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(5968..7098)
                     /gene="dnaJ"
                     /locus_tag="ACHKW5_003984"
     CDS             complement(5968..7098)
                     /gene="dnaJ"
                     /locus_tag="ACHKW5_003984"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO7513273.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(7187..9103)
                     /gene="dnaK"
                     /locus_tag="ACHKW5_003985"
     CDS             complement(7187..9103)
                     /gene="dnaK"
                     /locus_tag="ACHKW5_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO7513274.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            9480..9884
                     /gene="yaaI"
                     /locus_tag="ACHKW5_003986"
     CDS             9480..9884
                     /gene="yaaI"
                     /locus_tag="ACHKW5_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO7513275.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            9910..10623
                     /gene="msyB"
                     /locus_tag="ACHKW5_003987"
     CDS             9910..10623
                     /gene="msyB"
                     /locus_tag="ACHKW5_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7513276.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            10772..11338
                     /gene="satP"
                     /locus_tag="ACHKW5_003988"
     CDS             10772..11338
                     /gene="satP"
                     /locus_tag="ACHKW5_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO7513277.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(11373..11960)
                     /gene="mog"
                     /locus_tag="ACHKW5_003989"
     CDS             complement(11373..11960)
                     /gene="mog"
                     /locus_tag="ACHKW5_003989"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO7513278.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(12075..13028)
                     /gene="tal"
                     /locus_tag="ACHKW5_003990"
     CDS             complement(12075..13028)
                     /gene="tal"
                     /locus_tag="ACHKW5_003990"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7513279.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            13307..14737
                     /gene="yaaJ"
                     /locus_tag="ACHKW5_003991"
     CDS             13307..14737
                     /gene="yaaJ"
                     /locus_tag="ACHKW5_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO7513280.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            14807..15583
                     /gene="yaaA"
                     /locus_tag="ACHKW5_003992"
     CDS             14807..15583
                     /gene="yaaA"
                     /locus_tag="ACHKW5_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO7513281.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT         4060 a         3840 c         3644 g         4118 t
ORIGIN      
        1 acttatcaaa caccatcttt tcaaatggag aaattaataa tctcttcatt aaaaaaagca
       61 acatatttgg agcatcattc actaatacca gaataaaaaa tttgctctgt gtagatatta
      121 tgcctggtcg atggaccact caacttgtca ataagcactt aggttacaga tacacaggag
      181 tattcaaaac cctggcaagt attgatgaca aaccgtcccg tttcgaaatt ttgataccat
      241 tggtacaaac tttagttcgc gacaatgtaa agctaaataa tgatgtgtat aaagaactta
      301 ataagtttat gcacgattac gacaaaacat catcagaaat gagaaaatac cttaaaagca
      361 tcaacgagtg tatgttcctt atgaaaaaca tagctcatca aaattaaaaa atctcaaaaa
      421 agaagtaatt tcagaatgaa attaactgtg atgattatta attcactaca gaggaataaa
      481 aaataacctg aagcatactc tgaaacatcc tactctctca ctctaataat ggcaccttgc
      541 tcttcttata aagaagggaa taattaagaa tattaaacct tcatcaactt cacatcaaga
      601 aattaaaaat tttaaacatt aatctaaata ataacaacct gatcacataa tcgggtacga
      661 ttttatccga taactataat gctataactc tgggaaatgg ttggaataca ccgctgggtg
      721 ccatctcttt ttacgctgcc cgatcgagta gtaagttaaa taatgatacc agacacgaag
      781 gtacgagcta tcaggtcgcc tataataaat atctagtaca aacagccact cgatttagcg
      841 tggccgcctg gcgttatgct tcgcaggatt acaggacatt cagcgaccat ctttacgaaa
      901 atgataaaca ctatcatcag agtgattatg atgattttta tgatattggc agaaaaaata
      961 gcctttctgc taatattatg caacctttat ccaataatct gggaaatgta tcattaagtg
     1021 cgctttggcg gaattactgg gggcgaagtg gaaatgctaa agattaccaa ttcagttact
     1081 ccaataactg gcaacacatt agttatactt tctctgccag ccaatcttat gatgaaaata
     1141 ataaagaaga ggagcgtttt aatctgttta tctccattcc tttctactgg ggggatgata
     1201 ttgccaaaac acgtcaccaa attaacttat cgaattcgac ctcattttcc aaagatggct
     1261 attcccccaa caatactgga attactggca tagccggtga acatgatcag ttaaattatg
     1321 gtatatatgt taatcagcaa caacaaaata atgatacctc gcttggtacg aatttaagct
     1381 ggagaactcc catcgccata atagatggca gctatagtca ttctaaaaac gcctggcaaa
     1441 gtggtggaag tattagtagt ggattagttg tctggcccgg tggtattaat atcactaacc
     1501 agttatccga tacatttgca attctggatg cgtctgggtt agaaggtgca catattaatg
     1561 gacaaaaata caatcgaaca aacagcaaag gccaggttgt ttacgacctg ataatacctc
     1621 atcgtgaaaa ccatctggta cttgatatag caaacagtga aagtgaaaca gaattgcagg
     1681 gcaatcgtca aattattgca ccttaccgtg gagcagtttc ttatgtgcag tttacaactg
     1741 accaacgtaa gccctggtat atacaggcac tgcgtcccga tggttcgcca ttaacctttg
     1801 gctatgacgt actggatctc caggaaaata atattggagt cgttggccag ggtagtcgcc
     1861 tttttattcg cgtagatgaa attcccactg gaataaaagt tgctctcaat gatgaacaga
     1921 acttattctg tactattact tttcaacacg ttatcgatga aaacaaaaca tatatatgcc
     1981 agtaaaagga gtttactatg aagtggcttt tattaatcac cttgagtcta tattctttta
     2041 ttgtgcaaag tgctccctgt gcattgacca atgtcggtga acaacgtgga acatatatac
     2101 tcaagccact gagtatgaaa ggtaatctaa ccgccgaaaa attgaaaaca ccggaaatat
     2161 cattgagtgt acagacttgc aagagacaac caacgccatt aaagttcatt ttttaacaca
     2221 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     2281 tgacggtaaa aaaatagagc ttccagggaa gtcatcttat aatttagaag aaatgctatc
     2341 tgcgataaat ttcaccattc catattctat cgcatctgta tcagatagtt ctaacaccgc
     2401 agacattttt cctggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     2461 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     2521 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     2581 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     2641 cgagcaaggt gaattagttt ttgagcccag tgatgttgaa tattactttg aaccagtgac
     2701 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     2761 gggtcaaata ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     2821 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     2881 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     2941 ggttatgtac aactaatcta tctcccccac cttaactttg gggggaggtg ccgattaacg
     3001 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     3061 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     3121 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     3181 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     3241 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     3301 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg gtttttctgg
     3361 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     3421 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     3481 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     3541 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     3601 accaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     3661 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     3721 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     3781 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     3841 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     3901 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     3961 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     4021 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     4081 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     4141 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     4201 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     4261 ggcagaatgg aggtcaagcc atccagcgtg acaccttgca gtgaaacgcc agcattagca
     4321 aatgcaaaca gcggcaaaat cagatacgcc acccacgggt gtaacacatg ctccagtcgc
     4381 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     4441 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacactac gccaaccaga
     4501 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     4561 acagccgcga caccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     4621 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagcgctaac
     4681 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     4741 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     4801 gccggaacaa tcatcccacc aatagcggcg ataactggaa atgcggcctg gcgtaagctg
     4861 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     4921 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     4981 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctattg
     5041 gccataatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     5101 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     5161 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     5221 tttttacgat ccattgaaga atttgcgcca tcagcggtga gatgagtttt tgacggctgt
     5281 aagcgctcac gagtgaccct aagcccagat aatcatagct taaggtgagg atttctgaga
     5341 gattaattag gaaaagtaat aattaatttg ttgtcagtcg gcgatgtttg ttaggccaat
     5401 atttcaaaaa taatccagct tgtcgtcaat aattgcgatg aagagataaa ttaaagttaa
     5461 ataattaaat cattaaaaaa ttacgccata aaaatgcgag gcgtcttcag gattagaatc
     5521 gataaaacag aatgggttaa agagagcgat ataatagcgg cgggtgcttg aggctgtctg
     5581 tctcaggcat tagctgaacg gcagatagag aaaagccccg agtgatattt taccatcaac
     5641 ccgaggcccc ctatatgctg aacacatgta gagtgcctct tactgaccgt aaggtcaagg
     5701 agaagagagc aatgaagcag cataaggcga tgattgtcgc cctgatcgtc atctgtatca
     5761 ccgccgtagt ggcggcgctg gtaacgagaa aagacctctg tgaggttcac atccgaactg
     5821 gccagacgga ggttgctgtt ttcacggctt acgaatccga gtaagagcaa cggcggggag
     5881 tgatccccgc cactttacag gtgctcgcat atcttcaacg caccctattt ttacccaggc
     5941 ctgcccacgg gcaggctttt ggggaggtta gcgggtcagg tcgtcaaaaa acttcttcac
     6001 accatcaaag aagctctttg agcgcgggct gttgtgctca ccggttgggc caccgaagct
     6061 ttcttgcagc tcttgcagca gctgtttctg cttctcgttc aggcctaccg gtgtttcgac
     6121 gacaacgcgg cacagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc
     6181 gcgcatacgg aaaagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc
     6241 atcaagggtc ggtacttcga tttcgccacc cagcgccgcc atagcgaagt tgatcgggac
     6301 ttcgcaatag aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg
     6361 aacgtacaga tcgcctgccg gtgcgccatg ctcgcccgct tcaccttcgc ccgcaagacg
     6421 gatgcggtct ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc
     6481 aacacgacca tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg
     6541 acagtgtgga caggtctgct gtacagcgaa gaatccctga cgcatctgca cctgaccaga
     6601 accatgacag gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca
     6661 aacgtcacac tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc
     6721 ttcgagggtg agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc
     6781 acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc
     6841 gccgccaaaa ccgccgccgc ccataccacc ttgctcaaac gcagcatgac catactgatc
     6901 gtatgccgca cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa
     6961 tttcgcctcg gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg
     7021 tttgtaggcc tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta
     7081 ataatcttgc ttagccatct tttttaaatt gcccctagat gaatgcacgg gcggagagga
     7141 aattcccctt cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt
     7201 tgacttcttc aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat
     7261 cagcaccggc agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg
     7321 aaacctgtgc cagctcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag
     7381 cagtttccag tgcagtcagt gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt
     7441 cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag
     7501 tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca
     7561 ttttctggat ttcatcttcg ttcagaccag aagaagcctt gatggtgatc ttctgctctt
     7621 taccgctgtt tttatctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg
     7681 taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac
     7741 ccagagattt gttatcagcc gcacgtttac gttcaccctg cagcacatgg atggttaccg
     7801 cagactggtt gtcttcagcg gtagagaaca cctggctgtg cttggtcggg atagtggtgt
     7861 ttttcgcgat cagcgtcgtc atcacaccgc ccatggtttc gatacccaga gacagcgggg
     7921 taacgtccag cagcagtacg tctttcacgt caccagtcag aacaccaccc tgaacagcag
     7981 caccgattgc tacagcttcg tccgggttaa cgtctttacg cggctcttta ccaaagaact
     8041 cagcaacttt cttctgaacc attggcatac gagtctgacc accaacgagg ataacgtcgt
     8101 cgatatcaga tacggacagg ccagcgtcct gcagtgcaac tttcagcggc tcaatggaac
     8161 ggtttaccag atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt
     8221 gtttcggacc ggtcgcatct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcgg
     8281 aagacagttc gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat
     8341 cgttgcgcag gtcaatgccc tgatctttct tgaattcttc aaccaggtag ttgatcagac
     8401 ggctgtcgaa gtcttcacca cccaggtggg tatcaccgtt ggtagccaga acttcgaagg
     8461 ttttttcgcc gtcaacttcg tcgatttcga taatagaaat atcgaaagta ccaccaccca
     8521 ggtcataaac cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag
     8581 ctgcggtcgg ttcgttgatg atacgtttta cttccagacc agcgatacgg cctgcgtctt
     8641 tggttgcctg acgctgagca tcgttaaagt atgccggtac ggtgataaca gcttcagtta
     8701 ccggttcacc caagtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaga
     8761 tctgcggcgg tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag
     8821 caataatttt gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc
     8881 ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt
     8941 tagccggctg accaaccaga gtttcaccat cctgggtata ggcaatgata gaaggcgtgg
     9001 tgcgatcgcc ttcggcgttc tccagcacgc gaggagtggt gccatccata atcgctacac
     9061 aagagttggt agtacccagg tcgataccaa ttattttacc catctaaacg tctccactat
     9121 atattcggtc atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg
     9181 cccaatttca tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac
     9241 ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat
     9301 atagcaggac aagacgtgaa actgacagca attttattca tataaagaat gaatcgcagc
     9361 caagacattc atcaaaaaat tgtaataaaa agaaaacatt acgcacctga atcttctctt
     9421 tatcagcagt aaactagtgg gtatccatcc ccctacctct ccccactaag agaatcctta
     9481 tgaaatccgt ttttacgatt tccgccagcc tggcgattag tctgatgctg tgctgcacgg
     9541 cgcaggcaaa cgaccataaa atcctcggcg tcattgcaat gccgcgtaac gaaaccaacg
     9601 atctggcgtt gaaacttcct gtttgccgaa ttgttaaacg catacaactc tctgccgacc
     9661 atggcgattt acagttaagc ggtgcatcgg tttatttcaa agccgcccgt agcgccagtc
     9721 agagcctgaa tattccttca gaaataaaag aagggcaaac cactgactgg atcaacatta
     9781 acagcgataa cgacaataaa cgctgcgtct caaaaatcac cttttccggt catacggtga
     9841 actcatcgga tatggccacg ctgaaaatta tcggcgacga ctaacgccag aatatagaag
     9901 ccacaaaaaa tgaatgttaa ttacctgaat gattcagatc tggattttct ccagcattgc
     9961 agtgaggaac agttggcaaa tttcgcccga ttgctcaccc ataatgaaaa aggcaaaact
    10021 cgcctctcca gcgtactgat gcgcaacgaa ctgtttaaat cgatggaagg gcatcccgag
    10081 caacatcgcc gcaactggca gctgattgct ggagaattac agcattttgg tggcgatagt
    10141 atcgccaaca aactgcgcgg acacggtaaa ttgtatcggg ccattttgct cgatgtttca
    10201 aaacgattga agctgaaagc cgacaaagag atgtctacgt ttgagattga gcaacagtta
    10261 ctggaacaat ttctgcgtaa cacctggaag aatatggacg aggaacataa gcaggagttt
    10321 ctgcacgcga tcgatgccag ggtgaatgag ctggaagagc tgctgccgct gctgatgaaa
    10381 gacaaattat tggcaaaagg cgtgtcgcat ttgctttcca gccaactgac ccgcatttta
    10441 cgcacccacg cagcaatgag cgtacttggg catggtttgc tgcgcggcgc ggggctggga
    10501 ggccctgtag gtgcggcact aaatggggtt aaagcggtca gcggcagcgc ctatcgcgtg
    10561 acgattccag ccgtactgca aatcgcctgc ctgcgccgga tggttagcgc cactcaggtc
    10621 tgataccgga aaccggggaa aattttcatt ttcccctcaa aagatcgtag acactgcccc
    10681 actggctgat tattatgccg cgccctgaaa acactacagt tattcaggga aattatttca
    10741 ccattcattc gatgatgatt tttgaggaat tatgggcaac actaagttgg ctaatccggc
    10801 accgctgggc ctgatgggct tcggcatgac caccattctg cttaacctgc acaacgtggg
    10861 ttatttcgct ctggacggta ttattcttgc catgggcatt ttctacggcg gcatcgcgca
    10921 aatttttgct ggtctgctgg agtacaaaaa aggcaacact ttcggtttaa ccgcattcac
    10981 ctcttacggt tctttctggc tgacgctggt tgcgattctg ctgatgccga aactgggtct
    11041 gaccgatgcg ccaaatgcac agttccttgg tgtctacctg ggtctgtggg gcgtatttac
    11101 gctgtttatg ttcttcggca cgctgaaagg cgcacgcgtt ctgcaattcg ttttctttag
    11161 cctgaccgtg ctgtttgccc tgctggcgat cggtaacatt gccggtaacg ccgcaatcat
    11221 ccactttgcc ggctggattg ggctgatctg cggtgccagc gcaatctatc tggcgatggg
    11281 tgaagtactg aacgagcagt ttggtcgcac cgttctgccg attggtgaat cccactaatt
    11341 gttttgagat ccccccgctc ggggggattt ttttattcgc taacgtcgcg tcttgcactc
    11401 ttcggtctga atgctgcaac cacttccggt gccgtttcaa cgtatggccc ttccagcaac
    11461 tgaatgcagt acggtacgct ggcaaaaata ccgtgtacca caacgttacc cgcagcgtcc
    11521 ttcacacctt ccagcgtctc tttaatagac ttcggctgac cgggtaagtt aaggatcagc
    11581 gcctgtttgc gaatcacgcc cacctgacgc gaaaggatcg cagttggtac aaaatgcagg
    11641 ctgatctggc gcatctgttc accaaagcca ggcatctcgc ggtccgctac tgccagcgtc
    11701 gcatcgggcg ttacgtcacg acgcgccggg ccagttccgc ccgtggtgag caccagatgg
    11761 caactcattt catccactag ctcacacaac gtttgctcga tgatcgcctg ctcatcgggg
    11821 attaagcggg tttccagttc aaacggcgtg gttagcgccg atgtcagcca ttcttccagc
    11881 gcagggatgc ctttatcctg ataaacgccg ctggatgcgc gatcagagat ggaaactaag
    11941 ccaatacgta aagtattcat caagattttt ccgtttaaac agtctcgtta aacagaatga
    12001 tacactgcga agggagtgac agacaggctt cagaagaggt agcgtgaccg acttcccggt
    12061 cacgctaaga atgattacag cagatcgccg atcatttttt ccagtttttc ctggtcaata
    12121 gcaaacttac ggataccttc cgccagttta tctactgcca ttggatcctg gttgtgctgc
    12181 cacaggaact cggactcagt gatacgcgcc ggacgcgctt tcacttcgcc ggtgtaagac
    12241 agtttacgtt cgatagcccc ttcgctctcc gccagctctt tcagcagtgc cggtgcgatg
    12301 gtcagacggt cgcagcctgc cagttccaga atttcgccga tgttacggaa gcttgcgccc
    12361 ataaccactg tttcataacc gtgctctttg tagtactggt agatttcaga tacagaaacc
    12421 acgcccggat cttctgccgg agcgtactct ttcttatcag tattcgcttt gtaccagtca
    12481 agaatacggc caacaaatgg cgagatcagg aacacgcctg cttccgcaca agcacgagcc
    12541 tgagcgaagg agaacagcag ggtcaggtta cagttgatgc cttctttttc cagctgttct
    12601 gcagcacgga taccctgcca ggtagaagcc agtttgatca gaatacgatc gttgctaata
    12661 ccagcatcgt tgtagagttt gatcaggcgt tttgctttcg caattgacgc ttcggtgtca
    12721 taggaaagac gcgcatcaac ttcagttgag atacggcccg gaaccagttt caggatttcc
    12781 agaccaatat ttactgccag tttgtcggtc gcgtccacga tctgctgcgc gcgatcgttg
    12841 ctctgctgtt tcgcccaagc gacagcatca tcaatcaact tacggtattc cggaatctgc
    12901 gctgcgttaa gaatgagaga agggttggtt gtggcatcct gcggttgata cagcttcatt
    12961 gccgcgatgt ccccagtgtc ggccacaacg gtggtgtact gacgaaggga ggtcaatttg
    13021 tccgtcatga tagtatttct ctttaaacag cttgttaggg ggatgtaacc ggtctgccct
    13081 gatgatatca cgacgcaacg tcagcgcaac cgccgacaag acggttatcg ccaggcgaga
    13141 ctgtttcgga tttctgacac tgcgttgata tcgcccgcca tttttataca aaacctcatg
    13201 tatgctacgc agaagttatc aagtacctcg tagcgtatat acttcttaaa caattggtaa
    13261 cgtttacaca ggaaagtcat cgcgaccggc aataagaggg atatgcatgc cagatttttt
    13321 ctcctttatt aacagcgtcc tttggggatc ggtaatgatt tacctgctct tcggcgcagg
    13381 ttgttggttc acttttcgca ccggatttgt gcagtttcgc tacatccgcc agtttggcaa
    13441 aagtcttaaa aatagcattc atccacagcc aggcggttta acctcatttc agtcattgtg
    13501 taccagtctt gcggcgcgcg tgggtagcgg caacctggcc ggcgttgcgc tggctattac
    13561 cgccggtgga cctggagccg tcttttggat gtgggttgcc gcgtttatcg gcatggcgac
    13621 ctcgtttgcc gaatgttccc ttgcacaact ttataaagaa cgtgacgtca atggacagtt
    13681 tcgtggagga ccggcatggt atatggcgcg cgggctgggg atgcgttgga tgggcgttct
    13741 gttcgccgtc tttttgctca tcgcctacgg cataattttc agcggagttc aggcgaacgc
    13801 cgttgcccga gccctgagtt tttcttttga ttttcccccg ctggtgacag gcattattct
    13861 cgctgtcttt gctctgctgg caatcactcg cggtcttcat ggcgtcgccc ggctcatgca
    13921 gggttttgtc ccgttgatgg cgataatctg ggtactgacc agcctggtga tttgcgtaat
    13981 gaatatcggg caacttcccc acgtcatttg gtctattttt gagagtgctt ttggctggca
    14041 ggaagcggca ggcggcgcgg cgggatatac cttaagccag gcgattacta acggttttca
    14101 gcgcagtatg ttttccaatg aggcgggaat gggttctaca ccaaacgcag cagcggcagc
    14161 tgcgtcctgg cctccgcatc cggcagcgca agggattgtc cagatgattg gcatttttat
    14221 cgacaccctg gtcatctgta cggcaagcgc catgctgata ttactggcgg gtaacggcac
    14281 aacctacatg ccgctggaag gtattcagct tatccagaag gcgatgcggg tgctaatggg
    14341 ttcctggggt gctgagtttg ttaccctcgt ggttattctg tttgccttca gctccatcgt
    14401 tgccaactac atttatgccg aaaacaatct cttcttttta cgcctgaaca accctaaagc
    14461 gatctggtgt ttgcggatct gcaccttcgc aacggtcatc ggcggcacct tgctaagtct
    14521 tccgctgatg tggcaactgg cagatatcat aatggcctgc atggctatta ccaatttgac
    14581 cgccatttta ctgctctcgc ctgtggttca taccattgcc agtgattatc tacgccagcg
    14641 taaactcggc gtgcgcccgg tgtttgatcc gttgcgttat ccggatatcg gtcgccagct
    14701 ttctccggac gcgtgggatg atgtttcgca ggagtaatca caactatcga tcaactcatt
    14761 ctcttttttt gctaaagtcg gcataaattt cctgcaagga ctggatatgc tgattcttat
    14821 ttcacctgcg aaaacgcttg attaccaaag cccgttgacc accatgcgct atacgctgcc
    14881 ggagctgtta gacaattccc agcagttgat ccatgaagcg cggaaactga cgcctccgca
    14941 gattagcacg ctgatgcgca tcagcgacaa actggcgggt atcaacgccg ctcgctttca
    15001 tgactggcag ccagatttca cgccggagaa tgcccgccag gcgattctgg cgtttaaagg
    15061 tgatgtctac accggcttgc aggccgaaac cttcagcgaa gacgatttcg attttgccca
    15121 acagcatttg cgaatgcttt ccggcttgta tggcgtactg cgcccgctcg atttaatgca
    15181 gccttatcgt ctggaaatgg ggatccgtct tgagaatgcc cgagggaaag atctgtatca
    15241 attctgggga gatatcatca ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa
    15301 tgtggtgatt aatctggcgt cagatgagta ttttaaatcg gtgaagccga agaaattgaa
    15361 tgccgagatt atcaagccgg tgttcctcga tgagaagaac ggcaaattta agatcatcag
    15421 cttctacgct aagaaagcac gcggtctgat gagtcgtttc attattgaaa atcggctgac
    15481 caaaccagag caactgactg gttttaatag cgaaggttac ttctttgatg aagcttcctc
    15541 cagcaatggt gaactggtgt ttaaacgcta cgagcagcgt taagtttgta gccaatgccg
    15601 gatgcggcgt gaacgcctta tccggcctac aaaagcatgc agattcaata tattgcagaa
    15661 tt
//
LOCUS       DAZFYC010000116        15659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFYC010000116 DAZFYC010000000
VERSION     DAZFYC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.116"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            141..707
                     /gene="yceJ"
                     /locus_tag="ACHKW5_003993"
     CDS             141..707
                     /gene="yceJ"
                     /locus_tag="ACHKW5_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7513282.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            711..1286
                     /gene="yceI"
                     /locus_tag="ACHKW5_003994"
     CDS             711..1286
                     /gene="yceI"
                     /locus_tag="ACHKW5_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO7513283.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1328..2380)
                     /gene="trhO"
                     /locus_tag="ACHKW5_003995"
     CDS             complement(1328..2380)
                     /gene="trhO"
                     /locus_tag="ACHKW5_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO7513284.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2605..3525
                     /gene="lpxL"
                     /locus_tag="ACHKW5_003996"
     CDS             2605..3525
                     /gene="lpxL"
                     /locus_tag="ACHKW5_003996"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO7513285.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3697..4923
                     /gene="mdtG"
                     /locus_tag="ACHKW5_003997"
     CDS             3697..4923
                     /gene="mdtG"
                     /locus_tag="ACHKW5_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO7513286.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            5006..5380
                     /gene="msyB"
                     /locus_tag="ACHKW5_003998"
     CDS             5006..5380
                     /gene="msyB"
                     /locus_tag="ACHKW5_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7513287.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5381..5608)
                     /gene="yceK"
                     /locus_tag="ACHKW5_003999"
     CDS             complement(5381..5608)
                     /gene="yceK"
                     /locus_tag="ACHKW5_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7513288.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5781..8324)
                     /gene="mdoH"
                     /locus_tag="ACHKW5_004000"
     CDS             complement(5781..8324)
                     /gene="mdoH"
                     /locus_tag="ACHKW5_004000"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO7513289.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8317..9852)
                     /gene="mdoG"
                     /locus_tag="ACHKW5_004001"
     CDS             complement(8317..9852)
                     /gene="mdoG"
                     /locus_tag="ACHKW5_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO7513290.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10246..11403
                     /gene="mdoC"
                     /locus_tag="ACHKW5_004002"
     CDS             10246..11403
                     /gene="mdoC"
                     /locus_tag="ACHKW5_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO7513291.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11411..12892)
                     /gene="clsC"
                     /locus_tag="ACHKW5_004003"
     CDS             complement(11411..12892)
                     /gene="clsC"
                     /locus_tag="ACHKW5_004003"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO7513292.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12834..13367)
                     /gene="ymdB"
                     /locus_tag="ACHKW5_004004"
     CDS             complement(12834..13367)
                     /gene="ymdB"
                     /locus_tag="ACHKW5_004004"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO7513293.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13462..13773)
                     /gene="ymdA"
                     /locus_tag="ACHKW5_004005"
     CDS             complement(13462..13773)
                     /gene="ymdA"
                     /locus_tag="ACHKW5_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO7513294.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13894..14226)
                     /gene="csgC"
                     /locus_tag="ACHKW5_004006"
     CDS             complement(13894..14226)
                     /gene="csgC"
                     /locus_tag="ACHKW5_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO7513295.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14285..14740)
                     /gene="csgA"
                     /locus_tag="ACHKW5_004007"
     CDS             complement(14285..14740)
                     /gene="csgA"
                     /locus_tag="ACHKW5_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO7513296.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14781..15236)
                     /gene="csgB"
                     /locus_tag="ACHKW5_004008"
     CDS             complement(14781..15236)
                     /gene="csgB"
                     /locus_tag="ACHKW5_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO7513297.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3905 a         4000 c         3714 g         4040 t
ORIGIN      
        1 aaacttatga agattctttc attattaatt caaaattatt gaaataaaaa taaatatcat
       61 tcaatttgtt atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca
      121 aattcgcatt ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat
      181 ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt
      241 ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa ctgcataaaa
      301 gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc
      361 caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgca aaggctggac
      421 atctcgcatt gtacctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg
      481 ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg
      541 ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag
      601 tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca
      661 ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa
      721 gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg
      781 attacaaaat tgacaaagaa ggtcagcacg cctttgttaa tttccgcatc cagcaccttg
      841 gctatagctg gttatacggc acctttaaag atttcgacgg tacttttacc tttgacgaaa
      901 aaaatccggc tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaatc
      961 acgccgaacg cgataaacat cttcgcagtg cagatttcct caataccgca aaatatccac
     1021 aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg
     1081 atctgacgct gaatggcgta accaaacccg tcacgctgga agcgaaatta attggtcagg
     1141 gcgacgaccc atggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa attaagctca
     1201 aggacttcaa tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt
     1261 cagtggaagg cgtacagcag aagtaagagt gaaatgttgc cggatggcac gcatccggca
     1321 atgatattta ttccgttgga tcaggaatgc ccagtgttgt attcagacgt ccacgagact
     1381 tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg
     1441 gtaacgcgct ttcttcgcag caaatctcac tacaacaacc tttgtatttt tccgcacata
     1501 ctggacactg aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc
     1561 acggcgcgcc gcactggtgg caatgcgcga taatctcatc cgatatacgt tcgcccatcc
     1621 gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct
     1681 tacgggcgta ttcaataatt ccgccctcga tatgccagac tttgttgaat ccgttatgtt
     1741 tcatccaggc actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt
     1801 tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg
     1861 ccggaatttc gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt
     1921 cgataaatag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact
     1981 caccaacatt gctggcatca aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc
     2041 gtaccttcat gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc
     2101 gtaaaccctc taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc
     2161 tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa
     2221 cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt
     2281 ggaaatactt gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt
     2341 ttagcgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga
     2401 tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt
     2461 ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca
     2521 gtcttcagcc acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg
     2581 aataggttga aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt
     2641 catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt cgtgcaattg
     2701 ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg
     2761 aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa
     2821 caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa
     2881 accggcatgg cgtggttctg gccggaccgc cgaatcgccc gctggacgga agtgatcggc
     2941 atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat
     3001 tttctgacac tggagctggg tgcgcggcag tttggtatgc aggaaccggg gattggcgtt
     3061 taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca
     3121 aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc
     3181 gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg
     3241 ttatttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc
     3301 ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg
     3361 attatgctgc cgccagagtg ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg
     3421 atgaacaaag tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac
     3481 cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccctgccgga
     3541 tgcttcagaa tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct
     3601 ctctggattg cgccccctgg aagtcgggcg cataattagt gtgcttatct tttcttctta
     3661 tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc
     3721 cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggtgcc
     3781 gccttcagtc tggtaatgcc cttcttaccc ctctacgttg agcagcttgg cgttaccggt
     3841 cactccgccc tgaatatgtg gtccggtatt gtcttcagca ttacattttt attttcggcc
     3901 atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact catgctatta
     3961 cgctctgccc tcggcatggg catcgtgatg gtgttgatgg gactggcaca aaatatctgg
     4021 cagtttttga tcctacgggc gcttcttggg ttacttggcg gatttgtccc caacgctaat
     4081 gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcgctggg tacgctctcc
     4141 acaggcggcg ttagtggtgc gttgctcggc ccaatggctg gcggcctgct cgccgatagc
     4201 tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg ctttttcgtc
     4261 accctgttct gcatcagaga aaaattccag ccggtcagca aaaaagagat gctgcacatg
     4321 cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt tgtcaccacg
     4381 ttaatcatcc aggtggcgac aggctcaatt gcccccattc tgacgctgta tgtccgcgaa
     4441 ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc
     4501 gtggcggctc tgctaagcgc accacgactc ggcaaacttg gcgatcgaat cggacccgaa
     4561 aagatcctga ttacagcgct gatcttttct gtactgctgt tgatcccaat gtcttacgtt
     4621 cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga tggtgcacta
     4681 ctccccgccg tacagacact gttggtttac aactcgagca accagatcgc cgggcgtatc
     4741 ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt gatgggagca
     4801 gcgatttcag cgaactacgg tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta
     4861 ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac
     4921 tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc
     4981 tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag aagccattga
     5041 cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag atgcgaatgt
     5101 gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt ggcaagttga
     5161 gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg gtgaagccgc
     5221 gcaaagtgtt tttgatggcg actatgatga gatagagata cgccaggagt ggcaggaaga
     5281 gaatacatta catgaatggg acgaggggga atttcagctt gagccaccgc tggataccga
     5341 ggaaggacgc gcagcagctg atgagtggga tgaacgttaa tcactcatac gggccatgat
     5401 gaatgtcgat cggcagcagt aaagtatcaa aaaccagaga gaatggcaga tcaaggatcg
     5461 tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa tattggttgc
     5521 catgcccctg ccccggtata gtgcgactaa taatgctgcc acagccactc agcaaggtca
     5581 ccatgatgct caccacaatt aaacgcattt gatgcctctt tcgtaaaaaa ataccggcat
     5641 aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac gccttatccg
     5701 acccacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
     5761 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
     5821 ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg ggaattccag
     5881 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
     5941 cgattcagct tctctggcgt ctcgttcagc gcctgttcaa cgtggcggtc acgggcgatt
     6001 tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttaaat
     6061 gacgggttaa acactgcgtg cataaagcca tcatcaaggg agcgttgacg attcatctca
     6121 aggaaccgat cggtatcaac cagcacctgc ggcggcgaat actcttccgg gatcaggaac
     6181 agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
     6241 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccaac
     6301 cacgccatac caacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac
     6361 gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
     6421 ccaaggaacg cgctgacgac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
     6481 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
     6541 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
     6601 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     6661 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac ctgcaatgca
     6721 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     6781 aggaataccg cacggtgaac cgggtgcata cccttcacca ggaacagacg gaagttcatc
     6841 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     6901 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     6961 aacgccgctt ccacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     7021 agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc gttatgtccc
     7081 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     7141 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     7201 gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag gccgcataaa
     7261 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     7321 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     7381 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     7441 tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc aaagtgtttg
     7501 gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa aacacggttc
     7561 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     7621 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     7681 cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc
     7741 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     7801 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     7861 taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     7921 gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     7981 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     8041 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt cagctgatcg
     8101 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     8161 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     8221 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt
     8281 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     8341 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     8401 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     8461 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     8521 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     8581 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     8641 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     8701 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     8761 gacgatgtta tcgttggttt catcgttggt tggaatttct accagctcaa cgctgccttt
     8821 accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac gatcatcgag
     8881 atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt gcgggttttc
     8941 catagagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     9001 gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac gatagttatt
     9061 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     9121 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     9181 tggcataact acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat
     9241 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     9301 ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa ggccataaac
     9361 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     9421 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     9481 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     9541 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     9601 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     9661 aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct
     9721 tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     9781 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     9841 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     9901 tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat
     9961 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
    10021 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt tatttatcaa
    10081 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
    10141 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
    10201 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
    10261 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
    10321 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
    10381 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
    10441 atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
    10501 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccttact gacattgccg
    10561 caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg gctgtcattg
    10621 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac atctgtggtt tttactggtg
    10681 ttagtggtca tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa taatttagaa
    10741 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
    10801 ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc gcccattctg
    10861 agtaatggca tgttcaattt tattgtcatg caaacgctgt tttatttgcc gttctttatc
    10921 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
    10981 ggctgtaccc ttgcagcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
    11041 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg
    11101 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
    11161 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
    11221 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
    11281 atatttgtag tagggattgc gataattctg tatgaaattc atttgcgcat cccgttactg
    11341 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
    11401 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
    11461 gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt gcatgacgat
    11521 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc
    11581 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
    11641 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
    11701 taccatcgat gctaaaggtt ttagcatgca ggctggctcc ggaattaccg gttatgccgc
    11761 gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat tccacgccat
    11821 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
    11881 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
    11941 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
    12001 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
    12061 cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc ggcaacgttc
    12121 catcaaccag atgatttata aacggactgg attccatttt gcgtaagtaa cgatgcgtca
    12181 tagcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
    12241 catccagcac ttgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
    12301 cggcaacgtc ctctaccacg ggccctattg ccataacatc taaatccgaa aaaagtggct
    12361 cttcccctgc cccaaaatag gcatcaccaa tatttcgccc ccctaccagc gtcaccacgc
    12421 catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat
    12481 aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcggg
    12541 gatggctgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
    12601 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgac
    12661 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
    12721 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
    12781 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
    12841 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
    12901 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
    12961 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
    13021 actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc atcctgcaaa
    13081 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg cactacggct
    13141 ttagcgggaa gagcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
    13201 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
    13261 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
    13321 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
    13381 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
    13441 caacgtgcgt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
    13501 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttaacat taccgctcat
    13561 taacgctttg ctggaatagg ttttgcccgg aacagaacca ttctgtgggc agttcatttc
    13621 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
    13681 gatcaccccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa gcaaactgcc
    13741 gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
    13801 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
    13861 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcggcca
    13921 ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt tcacccgatc
    13981 ttccggggaa atatttaaac tcaacttcgt caaagcaata ggttgattag caggcaatga
    14041 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
    14101 ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg tatacacatc
    14161 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
    14221 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
    14281 tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
    14341 aggagttaga tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag
    14401 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
    14461 gggtcagatc gattgagctg tcatctgagc cctgacctac atctgcacca ttaccaccgc
    14521 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
    14581 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta ttaccgccac
    14641 caccgtggtt accgccgccg ccgtactgag gaacaacacc tgccagagcg ctaccggaga
    14701 atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc catcggattg
    14761 atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg cgactgtctc
    14821 tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt attaccagaa
    14881 cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact ggcatcgttg
    14941 gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc aatctttgcc
    15001 cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc ctgccgtaac
    15061 tgagcactat tattcgtccc agcttgacca attatggctg cctgattaaa tgaagactta
    15121 ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc tgctgcggct
    15181 gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt tttcatgttg
    15241 tcaccctgga cctggtcgta catttaagaa attaaatcat ttcaacttgg ttgttaacgc
    15301 aacctgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa atatttttaa
    15361 taactcaccc gcgcgttgta tttttctttt tttgttcata ccaaagtatt aaaacccacg
    15421 taaatacggt gttatctacg caaaaaatat tttgttttct tttaaatctc cgttttccgc
    15481 tatcaaaaat caccagacag tcatttttct tgcccgtcgc tgattgctgc gatatgtctt
    15541 gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac ggaggtgcgg
    15601 agaaaaaaca agaacgtttt acatgacgaa aggactacac cgaaatattt tttatatgt
//
LOCUS       DAZFYC010000117        15658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFYC010000117 DAZFYC010000000
VERSION     DAZFYC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.117"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1097)
                     /locus_tag="ACHKW5_004009"
                     /pseudo
     CDS             complement(1..1097)
                     /locus_tag="ACHKW5_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683738.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1296..1380)
                     /locus_tag="ACHKW5_004010"
     tRNA            complement(1296..1380)
                     /locus_tag="ACHKW5_004010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1344..1346),aa:Leu,seq:caa)
     gene            1576..2595
                     /gene="ahr"
                     /locus_tag="ACHKW5_004011"
     CDS             1576..2595
                     /gene="ahr"
                     /locus_tag="ACHKW5_004011"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO7513298.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2723..4225
                     /gene="yjgR"
                     /locus_tag="ACHKW5_004012"
     CDS             2723..4225
                     /gene="yjgR"
                     /locus_tag="ACHKW5_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO7513299.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGELNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLTLEQQGATHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4386..5468)
                     /gene="lptG"
                     /locus_tag="ACHKW5_004013"
     CDS             complement(4386..5468)
                     /gene="lptG"
                     /locus_tag="ACHKW5_004013"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7513300.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5468..6568)
                     /gene="lptF"
                     /locus_tag="ACHKW5_004014"
     CDS             complement(5468..6568)
                     /gene="lptF"
                     /locus_tag="ACHKW5_004014"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7513301.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6631..6681)
                     /gene="ytgA"
                     /locus_tag="ACHKW5_004015"
     CDS             complement(6631..6681)
                     /gene="ytgA"
                     /locus_tag="ACHKW5_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO7513302.1"
                     /translation="MSIIILATISTTQENK"
     gene            6835..8346
                     /gene="pepA"
                     /locus_tag="ACHKW5_004016"
     CDS             6835..8346
                     /gene="pepA"
                     /locus_tag="ACHKW5_004016"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO7513303.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8700..9143
                     /gene="holC"
                     /locus_tag="ACHKW5_004017"
     CDS             8700..9143
                     /gene="holC"
                     /locus_tag="ACHKW5_004017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO7513304.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9143..11998
                     /gene="valS"
                     /locus_tag="ACHKW5_004018"
     CDS             9143..11998
                     /gene="valS"
                     /locus_tag="ACHKW5_004018"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO7513305.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKLLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIDGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12054..13250)
                     /gene="yjgN"
                     /locus_tag="ACHKW5_004019"
     CDS             complement(12054..13250)
                     /gene="yjgN"
                     /locus_tag="ACHKW5_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO7513306.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYASDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            13443..13946
                     /gene="yjgM"
                     /locus_tag="ACHKW5_004020"
     CDS             13443..13946
                     /gene="yjgM"
                     /locus_tag="ACHKW5_004020"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7513307.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13992..14408)
                     /gene="rraB"
                     /locus_tag="ACHKW5_004021"
     CDS             complement(13992..14408)
                     /gene="rraB"
                     /locus_tag="ACHKW5_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO7513308.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14570..15583
                     /gene="argF"
                     /locus_tag="ACHKW5_004022"
     CDS             14570..15583
                     /gene="argF"
                     /locus_tag="ACHKW5_004022"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO7513309.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPKACWPEAALVTECRALAQQNGGDITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         4013 a         3999 c         4056 g         3590 t
ORIGIN      
        1 ctgccgctct accgcatccc tcgaccataa tcccgactca atcaacgaac tacaagccat
       61 cgtcctgaag ccatgcccac aaacctcgac cttcgtgtca taccccatta cgcgcaaagc
      121 cttgttcacc gtattctcac tcatcggctt acgtggatcg tgatcgccca caaaaatcag
      181 ctctcaattt ccgctcatgc ttttgatctt ttccaaaata cttaggacct gccgcgacaa
      241 aggtacaagg tgtggtgtcc gcatcttcga gccacgctga gaatgcttga ctccttccag
      301 ttgctcacgc tcacctggga ttgtccacat agccgtttca aaatctactt ctgaccagcg
      361 ggcaaaatgc agctcgctcg aacggatgaa aaccaataaa gtgagttcaa ccgccagccg
      421 agttaacggt ctgccagaat agtgatctat gcgatgaagt aattcaggta tacggttcag
      481 ttcaagagct gcacggtgct gtcttttcgc cgtagcaacc gcacctgcaa tctcttgcgc
      541 ggggttgtag tcgattaagc cgctctgaac ggcaaagcgc ataatcgcgg tagtacgctg
      601 ctgtaaacgg gcggcaactt cgagccgccc ggatgactcg actgctttaa tgggtacaag
      661 aagatcccgc gtcttcagtt ccgcaatgtt ccgcttaccg atagcagtaa agagattatc
      721 ctccagactt ttcaaaacac gagcgctatg cgatgccgac cacttctgat tgctggcgtg
      781 ccagtctctg gcgactactt caacgttatc gcctcttgtt cctgttctac cttaacagct
      841 ttcttgtttt cactgggatc gacaccatta gctaaaagct tacgagcctc atcccggcgt
      901 gccctggcat ccgccaacga cacttcgggg tatttcccca atgccagcat cttctcttta
      961 ccgccaaagc gataacgtag ccgccagtat ttggagccgt tagggtgaac cagcaaaacc
     1021 atgccttcac cgtcggtcag cttataggct tttgcttcag gcttagccga acgaaccttc
     1081 acatcactca gagccatgat gagtatcctt tcaagggttc tgtgtgggta caaacattat
     1141 cgaaccggga aatacccgca gttgtacccg catcagtaag ttgatgtaga ttgaatcagg
     1201 ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg atactggatt ttaggcacaa
     1261 aaaaagacgt ccgttgacgt ctattgatat tccgatggtg ccgaaggccg gactcgaacc
     1321 ggcacgtatt tctacggttg attttgaatc aactgcgtct accgatttcg ccacttcggc
     1381 actgaagagg tatgcggaaa acgttgtgga ttatacctgt cgcacgccac catgcaagcg
     1441 ccagcttgca cctacctcgc taagtgctga aattttcagc attacactca gcgatatgta
     1501 acctttgtca cactccaggc accccgccct gccatgctct acacttccca aacaacacca
     1561 gagaaggacc caaaaatgtc gatgataaaa agctatgccg caaaagaagc gggcggcgaa
     1621 ctggaagttt atgagtacga tcccggtgag ctgaagccac aagatgttga agtgcaggtg
     1681 gattactgcg ggatttgtca ttccgatctg tcgatgatcg ataacgaatg gggattttca
     1741 caatatccgc tggttgccgg gcatgaggtg attgggcgcg tggtggcact cgggagcgcc
     1801 gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga ttggctggac ggcgcgtagc
     1861 tgtggtcact gcgacgcctg tattagcggt aatcagatca actgcgagca aggtgcggtg
     1921 ccgacgatta tgaatcgcgg tggctttgcc gagaagttgc gtgcggactg gcaatgggtg
     1981 attccactgc cagaaaatat tgatatcgag tccgccgggc cgctgttgtg cggcggtatc
     2041 acggtcttta aaccactgtt gatgcaccat atcactgcta ccagccgcgt tggggtaatt
     2101 ggtattggcg ggctggggca tatcgctata aaacttctgc acgcaatggg atgcgaggtg
     2161 acggccttta gttctaatcc ggcgaaagag caggaagtac tggcgatggg tgccgataaa
     2221 gtggtgaata gccgcgatcc gcaggcactg aaagccctgt cggggcagtt tgatctcatt
     2281 atcaatactg tgaacgtcag cctcgactgg cagccttatt ttgaggcgct gacctatggc
     2341 ggtaatttcc atacggtcgg tgcggttctc acgccgctgt ctgttccggc ctttacgtta
     2401 attgcgggcg accgcagcat ctctggttct gctaccggca cgccttatga gctgcgaaag
     2461 ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa ccgaactgtt cccgatgtcg
     2521 aaaattaacg acgccattca gcatgtacgc gatggtaaag ctcgctaccg agtagtcctg
     2581 aaagccgact tctgatccct ttgccgggtg ctgtcgcccg gcacttaaat ctctttggaa
     2641 ggcgtctcgc aatctgctga atttagcggt gaagagtgca cagccgttgc ctatactcca
     2701 ggcaggaact ggaggaaatc tcatgagcga acccctgtta attgcccgca cgccggacac
     2761 agaactgttt ttactgccgg gaatggctaa ccgtcacggg ctgattactg gcgcaacggg
     2821 gacgggtaaa accgttacgc tgcaaaaact ggcagagtca ttgtcggaaa tcggcgtgcc
     2881 ggtgtttatg gctgatgtga aaggcgatct gaccggtatc gcgcaggcag gaacggcgtc
     2941 ggaaaaactg ctcacaaggc ttaaaaatat cggcgtcaat gactggcaac cgcatgccaa
     3001 tccggtggtg gtgtgggata tctttggcga gaaaggccat ccggtgcggg cgacggtttc
     3061 ggatctgggg ccgctgttgc tggcgcggct gttgaatctc aacgatgtgc aatctggcga
     3121 gctaaatatc atcttccgca ttgctgacga tcaggggctg ttactgctcg actttaaaga
     3181 tttgcgggcg attacccagt acatcggcga taacgccaaa tcattccaga atcagtacgg
     3241 taatattagt agcgcatcgg ttggtgccat ccagcgcgga ttactgacgc tggaacagca
     3301 aggggcaacg catttctttg gtgagccgat gctggatatc aaagactgga tgcgtactga
     3361 tgccaacggt aaaggcgtta tcaatatcct cagcgccgag aaactttatc agatgccgaa
     3421 actgtacgcc gccagcctgc tgtggatgct ttcagagttg tatgaacaat tgccggaagc
     3481 gggcgatctg gagaaaccaa aactggtgtt tttcttcgac gaggcacatc tgctgtttaa
     3541 cgatgcaccg caggtactgc tggataagat tgagcaagtg ataaggctta ttcgctcaaa
     3601 aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat attccggata atgtgctcgg
     3661 gcagctcggt aatcgcgttc aacacgcttt gcgggctttt acgcccaaag atcagaaagc
     3721 ggtaaaagct gcggcgcaaa ccatgcgggc caatccggca tttgataccg aaaaggcaat
     3781 ccaggaactg gggaccggcg aggcgttaat ctcgtttctc gatgcaaaag gaagtccttc
     3841 tgtggtggaa cgggcgatgg tgattgcgcc ttgttcgcgg atgggaccgg tgacggaaga
     3901 tgagcgtaat ggcttgatta atcactctcc ggtgtatggc aagtacgagg atgacgtgga
     3961 ccgggaatcc gcctatgaga tgttgcaaaa aggcgtacag gcaagcaccg agcagcaaaa
     4021 taatcctgcc gtgaaaggga aagaggtggc ggtggatgac ggcattcttg gtggattgaa
     4081 ggatattttg ttcggcacta ccggaccacg cggcgggaag aaagatggtg ttgtgcaaac
     4141 aatggcgaaa agcgccgccc gccaggtgac gaatcagatt gtgcgtggaa tgttggggag
     4201 tttgctggga gggagaagaa ggtaagtcag gtgcgatttg tctttgcctg atgcgacgct
     4261 ggcgcgtctt atcatgccta caccactgca atatattgga ttttcatgat ttttcaggca
     4321 tgaacgaagc acactttgct aacaataata aagggagctt tcgctccctt tattcgttca
     4381 tcaggttacg attttctcat taacagccac aggctgatta agaagaagct ggcgcttggc
     4441 aacagtgcgc cgatgatcgg cgggatgcca taaaccaacg tcagcgggcc gaagatctgg
     4501 tccagtacgt agaagacaaa accgaagctg ataccggtga ccacacgcac gcccatcggt
     4561 acgctacgca gtgggccaaa gatgaacgac agcgccatca gcatcatcac cgccacggat
     4621 agcggctgga agattttgct ccacatattg agctgataac gtccggcatc ctgaccgctc
     4681 gacttcagat acttcacgta gttgtgcaaa ccgctgattg agagtgcatc cgggtccagc
     4741 gccaccacgc ccagtttgtc tggcgtgagg ttggttttcc aggtgccgct caccgtctgc
     4801 gaaccggtga tctgtttcgg attggtcaga tcagattcat caacctgcga cagacgccag
     4861 actttatgtt ccgggtcaaa cttcgcggta gcggcatagc gtacggattg cagacgacga
     4921 ttctcgttaa aggcatagat actgatgcca cctaactctt cgtcaccttt aacccgctca
     4981 atgtagacga agttgttgcc atctttcgcc cataagcctt gctgggtaga gagcaacgag
     5041 ccgccgtaca tcgcctgcgc acggtagtta cgcgccatct gctcgccctg cggcgcgacc
     5101 cattcgccaa tcgccatcgt cagcaagacc agtggaatgg cggttttcat caccgacagc
     5161 gccacctgca tacgggtaaa accagaagcc tgcatcacca ccagttcgct gcgctgcgcc
     5221 agcatcccaa gaccaagcaa cgccccaagc agagccgcca tcgggaagaa gatttgcaca
     5281 tctttcggca cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta actcccctgc
     5341 ccggcttttt tcagctgatc gacaaacttg ataatgcccg acagcgacac cagcatgaac
     5401 agcgtcatca tgatggtggt gaaaatagtt ttaccgatat agcggtcaag tacgccaaaa
     5461 ggttgcatca caccgctcct ttacgcgaaa aactggcgcg caggcggcgg accggcacgg
     5521 tgtcccaaag gttgagaaca atcgccaaag ccagataaat caggttaacg gtccacatcc
     5581 acagcgtcgg gtccagctta cctttaccgc cgttcgattt cagggaggtc tggatcagga
     5641 agaaaagtag atacagcagc atggctggca gcatcgacag tacgcgtccc tgacgcgggt
     5701 taaccacgct cagcggtacg accataagtg ccatcataaa cacggtgaat accaacgtga
     5761 tacgccagtt cagttctgcg cgagcacgat cggtatcagt gttccacaat gtacgcatgt
     5821 ccatctggtc ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg atcgcctgat
     5881 aatcctggaa gtccgtaatg cggaaatcac gtaacagtgc agtgccttcg aagcgcgttc
     5941 cctggttgag agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt ccggaatcgg
     6001 ccaccaccac agaaggacga gcattacctt ttggtcgaat ttgcgcgagg aacacatctt
     6061 tgaaatcgct gccgtcaacg ctttcgatga acagcaccga gctgccatta gtcgcttgct
     6121 ggaattgccc ttgcgccagc gccgccatgc cagggttcgc tttcgcttct gctaacactt
     6181 catcctgatg acgcgatgac cacggtcccg cccacatcac gttaaccgcc gcgacgattg
     6241 ccgtgaatac cgcaaggatc attgccgctt tcaccagaac cgctttgctc aggccgcagg
     6301 catgcattac cgtaatttca ctttcggtat acagtttgcc cagcgtcatc agcagcccga
     6361 ggaacaggct taatggcagg ataagctgcg ccatttccgg cacgcccaac ccgagaaggg
     6421 agagcaccag attcgccgga atatcgccgt caaccgctgc gccgaggatc ctcactaact
     6481 tttgacagaa gaagatcaaa agcaagatga agagtatcgc cagctggctt ttgagcgtct
     6541 cccgcaccag atatcttatg attatcactt taaatacgcc cgtaaaaact cgtcttttgc
     6601 aggattttag cttgtttcat ggcttaaacg tcatttattc tcttgagtcg tcgaaatcgt
     6661 cgctaagata attatactca acggattcac ctctcagatt ttgttctgac gtgccaatgc
     6721 cgtaataacg ttaagattaa cacgaagtca tcgcaacagc ggacatgagt tacgaaagct
     6781 tgcaattcta tctgtagcca ccgccgttgt ctttaagatt caggagcgta gtgcatggag
     6841 tttagtgtaa aaagcggtag cccggagaaa cagcggagtg cctgcatcgt cgtgggcgtc
     6901 ttcgaaccac gtcgcctttc tccgattgca gaacagctcg ataaaatcag cgatgggtac
     6961 atcagcgccc tgctacgtcg gggcgaactg gaaggaaaac cggggcagac attgttgctg
     7021 caccatgttc cgaatgtact ttccgagcga attctcctta ttggttgcgg caaagaacgt
     7081 gagctggatg agcgtcagta caagcaggtt attcagaaaa ccattaatac gctgaatgat
     7141 actggctcaa tggaagcggt ctgctttctg actgaactgc acgttaaagg ccgtaacaac
     7201 tactggaaag tgcgtcaggc tgtcgagacg gcaaaagaga cgctctacag tttcgatcag
     7261 ctgaaaacga acaagagcga accgcgtcgt ccgctgcgta agatggtgtt caacgtgccg
     7321 acccgccgtg aactgaccag cggtgagcgc gcgatccagc acggtctggc gattgccgcc
     7381 gggattaaag cagcaaaaga tctcggcaat atgccgccga atatctgtaa cgccgcttac
     7441 ctcgcttcac aagcgcgcca gctggctgac agctacagca agaatgtcat tacccgcgtt
     7501 atcggcgaac agcagatgaa agagctgggg atgcactctt atctggcggt cggtcagggt
     7561 tcgcaaaacg aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc ggaagatgca
     7621 cgcccaatcg tgctggtggg taaaggttta accttcgact ccggcggtat ctcgatcaag
     7681 ccttcagaag gcatggatga gatgaagtac gatatgtgcg gcgcggcggc ggtttacggc
     7741 gtgatgcgta tggtcgcgga gctacaactg ccgattaatg ttatcggcgt gctggcaggt
     7801 tgtgaaaaca tgccaggcgg tcgcgcctat cgtccgggcg atgtgttaac caccatgtct
     7861 ggtcaaaccg ttgaagtgct caacaccgac gctgaaggcc gcctggtact gtgcgacgtg
     7921 ttaacttacg ttgagcgttt tgagccagaa gcggtgattg acgtggcgac gctgaccggt
     7981 gcctgcgtga tcgcgctggg tcatcacatt actggtctga tggcaaacca caatccgctg
     8041 gcccatgaac tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg cttaccgctg
     8101 ggtgacgagt atcaggaaca actggagtcc aattttgccg atatggcgaa cattggcggt
     8161 cgtcctggtg gggcgattac cgcaggttgc ttcctgtcgc gctttacccg taagtacaac
     8221 tgggcgcacc tggatatcgc cggaaccgcc tggcgttctg gtaaagcaaa aggcgcaacc
     8281 ggtcgtccgg tagcgttgct ggcacagttc ctgttgaacc gcgctgggtt taacggcgaa
     8341 gagtaattgc gtcaggcaag gctgttattg ccggatgcgg cgtgaacgcc ttatccgacc
     8401 tacacagcac tgaactcgta ggcctgataa gacgcgcaag cgtcgcatca ggcaatgctg
     8461 ttattgtcgg atgcggcatg aacgccttat ccgacctaca cagcaccgaa ctcgtaggcc
     8521 tgataagacg cggcagcgtc gcatcaggca atgctgttat tgtcggatgc ggcgtgagcg
     8581 ccttatccga cctacacaca gcactgaact cgtaggcctg ataagacaca acagcgtcgc
     8641 atcaggcgct gcggtgtata cctgatgcgt atttaaatcc accacaagaa gccccatata
     8701 tgaaaaacgc gacgttctac cttctggaca atgacaccac cgtcgatggc ttaagcgccg
     8761 ttgagcaact ggtgtgtgaa attgccgcag aacgttggcg cagcggtaag cgcgtgctca
     8821 tcgcctgtga agatgaaaag caggcttacc ggctggatga agccctgtgg gcgcgtccgg
     8881 cagaaagctt tgttccgcat aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg
     8941 agatcgcctg gccgcaaaaa cgcagcagca gcccgcgcga tatattgatt agtctgcgaa
     9001 caagctttgc agattttgcc accgctttca cagaagtggt agacttcgtt ccttatgaag
     9061 attctctgaa acaactggcg cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc
     9121 tgaatacggc aacctggaaa taatggaaaa gacatacaac ccacaagata tcgaacagcc
     9181 gctttacgag cactgggaaa agcagggcta ctttaagcct aatggcgatg aaagccagga
     9241 aagtttctgc atcatgatcc cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc
     9301 cttccagcaa accatcatgg ataccatgat ccgctatcag cgcatgcagg gcaaaaacac
     9361 cctgtggcag gtcggtactg accacgccgg gatcgctacc cagatggtcg ttgagcgcaa
     9421 gattgccgca gaagaaggta aaacccgtca cgactacggc cgcgaagctt tcatcgacaa
     9481 aatttgggaa tggaaagcgg aatccggcgg cacgattacc cgtcagatgc gccgtctcgg
     9541 caactccgtc gactgggagc gtgaacgctt caccatggac gaaggcctgt ccaatgcggt
     9601 gaaagaagtt ttcgttcgtc tgtataaaga agacctgatt taccgtggca aacgcctggt
     9661 aaactgggat ccgaaactgc gcaccgctat ctctgacctg gaagtcgaaa accgcgaatc
     9721 gaaaggttcg atgtggcaca tccgctatcc gctggctgac ggtgcgaaaa ccgcagacgg
     9781 taaagattat ctggtggtcg cgactacccg tccagaaacc ctgctgggcg atactggcgt
     9841 agccgttaac ccggaagatc cgcgttacaa agatctgatt ggcaaatatg tcattctgcc
     9901 gctggttaac cgtcgtattc cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac
     9961 cggctgcgtg aaaatcactc cggcgcacga ctttaacgac tatgaagtgg gtaaacgtca
    10021 cgccctgccg atgatcaaca tcctgacctt tgacggcgat atccgtgaaa gcgcccaggt
    10081 gttcgatacc aaaggtaacg aatctgacgt ttattccagc gaaatccctg cagagttcca
    10141 gaaactggag cgttttgctg cacgtaaagc agtcgttgcc gcagttgacg cgcttggcct
    10201 gctggaagaa attaaaccgc acgacctgac cgttccttac ggcgaccgtg gcggcgtagt
    10261 tatcgaacca atgctgaccg accagtggta cgtgcgtgcc gatgtactgg cgaaaccggc
    10321 ggttgaagcg gttgagaacg gcgacattca gttcgtaccg aagcagtacg aaaacatgta
    10381 cttctcctgg atgcgcgata ttcaggactg gtgtatctct cgtcagctgt ggtggggtca
    10441 ccgtatccct gcatggtacg acgaagcagg taacgtttat gttggccgca acgaagaaga
    10501 agtgcgcaag gaaaataacc tcggtgccga cgttgccctg cgtcaggacg aagacgttct
    10561 cgacacctgg ttctcttctg cactgtggac cttctctacc cttggctggc cggaaaacac
    10621 cgacgccctg cgtcagttcc acccaaccag cgtgatggta tccggcttcg acatcatctt
    10681 cttctggatt gcccgcatga tcatgatgac catgcacttc atcaaagatg aaaatggcaa
    10741 accgcaggtg ccgttccaca ctgtttacat gaccggtctg attcgtgacg acgaaggcca
    10801 gaagatgtcc aaatccaagg gtaacgttat cgacccgctg gatatggttg acggtatttc
    10861 gctgccagaa ctgctggaaa aacgtaccgg caatatgatg cagccgcagc tggcggacaa
    10921 aatccgtaag cgcaccgaga aacagttccc gaacggcatt gagccgcacg gcaccgatgc
    10981 cctgcgcttc accctggcgg cgctggcgtc taccggtcgt gacatcaact gggatatgaa
    11041 gcgcctggaa ggttaccgta acttctgtaa caagctgtgg aacgccagcc gctttgtgct
    11101 gatgaataca gaaggtcagg attgcggctt caacggcggt gaaatgacgc tgtcgctggc
    11161 ggaccgctgg attctggcag agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga
    11221 cagcttccgc ttcgatatcg ccgcaggcat tctgtatgag ttcacctgga accagttctg
    11281 tgactggtat ctggagctga ctaagccggt aatgaacggt ggcaccgaag ccgaactgcg
    11341 cggtactcgc catacgctgg tgaccgtgct ggaaggtctg ctgcgcctcg cgcatccgat
    11401 cattccgttc atcaccgaaa ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc
    11461 cgacaccatc atgctgcagc cgttcccgca gtacgatgca tctcaggttg atgaagccgc
    11521 actggccgac acagagtggt tgaagcaggc gatcgttgcg gtacgtaaca tccgtgcaga
    11581 aatgaacatc gcgccgggca aactgctgga gctgctgctg cgtggttgca gcgcggatgc
    11641 agaacgtcgc gtaaatgaaa accgtggctt cctgcaaacc ctggcgcgtc tggaaagcat
    11701 caccgtgctg cctgccgatg acaaaggtcc ggtttccgtt acgaagatca tcgacggcgc
    11761 agagctgctg atcccgatgg ctggcctcat caacaaagaa gatgagctgg cgcgtctggc
    11821 gaaagaagtg gcgaagatcg acggcgaaat cagccgtatc gagaacaaac tggcgaacga
    11881 aggctttgta gcgcgcgcac cggaagcggt catcgcgaaa gagcgtgaga agctggaagg
    11941 ctatgcagaa gcgaaagcga agctgattga acagcaggct gttatcgccg cgctgtaatc
    12001 aaatcacagc gatgaaaaag aaggccggag catactccgg ccttttttat ctcttacaga
    12061 aatggtaaag aaggcataat tccccgtgaa atcctggcta agaagccttt atctggttgt
    12121 tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt attttttgcc
    12181 tgccagtcaa tcatccatat tttcagcagt ggataagcca gaccgcccgt aatcccggat
    12241 atcaccacca acgcacacat gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa
    12301 cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt
    12361 aaataactgg tgctaacagc aatcccaaaa taatagatta actgcgcgat tatcatttta
    12421 cgttgcattt ccataaattg ctgtaaattc tcaatatcat cacttgcata cacagaacta
    12481 tcatacgcat ttaatatttg atcgaagata atataaccag caacagcgag aaaaggtaat
    12541 aaagctaaaa atgcgagaat ggcatatttt atacaatacg tagtatctaa tttcaccttg
    12601 aaacgatgta taccgaaact ggtattgctc cagagaaaac tcattaccag actatataaa
    12661 gtaccattaa aaataccaat ggaaacaata ccaactattg ccatcagaac cacagatata
    12721 ataacactac ttgaactatt ggcatgtagc atctttgtcg agataaagaa aacagtcccc
    12781 atcccaatgg ccattaaaat cggcaagaaa aaggttaccc accagaaccc tttcaaagag
    12841 caattaaaac taaatcttac gccgttgaga ctggtcatca aggcctgata acgcagtcct
    12901 tttgctgcca taaatataag caaaaccaac agtgacaaag tcaaaacaca gccaacaagc
    12961 ggcatatctg ctgacactgt cattaagatt gcgaaataga aaaaaacaaa aacaagacaa
    13021 ctaacaaaaa cattcccacc ggtaattcca taagaaaatc gttgtccgtt aacttccata
    13081 ttagcataga gataacgctt acatttcatt aatgcccatg gtaaatagat acctagcgtt
    13141 ataatcgtta acaacacatt caccacacaa ataagaaaat atctctcacc tgtgccatga
    13201 aaaacaaacg aatgggacgg aacatccatt tcattaataa cttgagccat aaaaattccc
    13261 tgaattaaaa aatccattga tataatttat agatacagac agtaaaatgt tctgccacgt
    13321 tacaatactc ttatcagtaa taggctggca aaaactttat tactttataa gtcgcttgcg
    13381 cacgtaagaa gagagtggta ttaaacaaat gtataattac tttattttta ttgggttaaa
    13441 ctatgaataa cattgcgccg caatcacctg taatgcgtcg cctgacgcag caggataatc
    13501 ccgctatcgc ccgcgtcatt cgtcaggtat ccgccgaata cggtcttacc gctgataaag
    13561 gctacaccgt cgccgatccg aaccttgacg agttgtatca agtgtatagt cagcctggcc
    13621 atgcatattg ggtggttgag ttcgatggtg aagtggtcgg cggcggtggg attgcgccat
    13681 tagcaggtag tgaatcggac atttgcgaac tgcaaaagat gtattttctt ccggctattc
    13741 gcggtaaagg gctggcgaaa aaactggcct taatggcgat ggagcaggca cgagagatgg
    13801 gcttcaaacg ctgctatctg gaaacgaccg cttttttaaa ggaagccatt gcgctttatg
    13861 agcatttggg ctttgagcat atcgactatg cgcttggctg cacgggccat gtcgattgtg
    13921 aagtgcggat gctacgcgaa ctctaaaaaa atgcccgcta gtgtgtgcta tcgggcattt
    13981 taatcaatta attagtggcg aaccccgtca tcgtcttcat cgacaaaatc ttcatcgtcg
    14041 ccgtcttcgc cgttaggatc ttcaaagtaa gtgccccaac cgtcgtactc aacgtcaaat
    14101 ttctccgcca gcgtcatcag ttgttcaacc tgggcatcga tcagatcggc attcaacgcg
    14161 cactcgctga ggatgtcgca gcaaatcacg atatcaccat cttcaacttc cagctcttct
    14221 ggatcggtca cttcataacc aagtttaaac gcttcaactg ctgctttttc cagggtttcc
    14281 agatcgtctg cggaaagatg gtgttcgatg gtgtacagcg cgtccggatc gctgccatct
    14341 tccagtaatt cttcaataat caaacgtgtt tcttcacgct gttcttccag ttgttccggg
    14401 tttgccatgg ctcattcctc ttaatgtcct gccgatactc ttattgtcac acaccactga
    14461 cattgcctcc acctttgtga caaagattta tgctttagac ttgcaaatga ataaccatcc
    14521 atataaattg aattttaatt cattgaggcg ttagccacag gagggatcta tgtccgggtt
    14581 ttatcataag catttcctga aattactcga tttcacgcca gctgaactca acagcctgct
    14641 gcagttagcc gcgaagctga aagccgataa gaaaagtggt aaagaagaag ccaaactcac
    14701 tggtaaaaac atcgcgctca tcttcgaaaa agactcgact cgtacccgat gctctttcga
    14761 agttgccgca tatgaccagg gtgctcgcgt tacttatctc ggcccaagcg gcagccagat
    14821 tggtcataaa gagtcgatta aagacactgc ccgcgtgctt ggtcgcatgt atgacggtat
    14881 tcagtatcgc ggctatggtc aggagattgt cgaaacactg gcggaatacg ctggcgtgcc
    14941 agtatggaac ggcctgacca atgagttcca ccccacccag ctgctggcgg atcttctcac
    15001 catgcaagag catttgcccg gtaaaacatt caacgaaatg acgctggtct atgcaggtga
    15061 cgcacgtaac aacatgggaa actcgatgct tgaagctgcg gcgcttaccg gtctggattt
    15121 gcgtctggtc gcgccaaagg catgctggcc ggaagctgcg ctggttacgg aatgccgcgc
    15181 cctggcacag caaaatggcg gggatattac gctgacggaa gatgtagcca aaggagttga
    15241 aggtgctgac tttatctata ccgatgtatg ggtgtcgatg ggtgaagcaa aagagaaatg
    15301 ggcggaacgc attgcattgc tgcgtgatta tcaagtgaac agcaagatga tgcagttgac
    15361 cggtaacccg gaggtcaaat tcctccactg cctgcccgcg tttcatgacg accaaacgac
    15421 gcttggcaag aaaatggcgg aagagtttgg cctacatggc ggaatggaag tgactgatga
    15481 ggtcttcgaa tctgccgcca gcattgtatt tgatcaggcg gaaaaccgca tgcataccat
    15541 caaagcggtg atggtcgcga cgctcagtaa attaaataat taatatctct atcaagatgc
    15601 cgggaaatac aaaaacatcc cggcatcaca ttattttatg cagtgatagt tttttctt
//
LOCUS       DAZFYC010000118        15403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFYC010000118 DAZFYC010000000
VERSION     DAZFYC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.118"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..329
                     /locus_tag="ACHKW5_004023"
     CDS             81..329
                     /locus_tag="ACHKW5_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7513310.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHKW5_004024"
     CDS             307..1437
                     /locus_tag="ACHKW5_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO7513311.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKW5_004025"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKW5_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO7513312.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHKW5_004026"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHKW5_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKW5_004027"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKW5_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO7513313.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKW5_004028"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKW5_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO7513314.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKW5_004029"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKW5_004029"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO7513315.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKW5_004030"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKW5_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO7513316.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKW5_004031"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKW5_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO7513317.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5872..7125
                     /gene="kch"
                     /locus_tag="ACHKW5_004032"
     CDS             5872..7125
                     /gene="kch"
                     /locus_tag="ACHKW5_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO7513318.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7180..7353)
                     /gene="yciY"
                     /locus_tag="ACHKW5_004033"
     CDS             complement(7180..7353)
                     /gene="yciY"
                     /locus_tag="ACHKW5_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO7513319.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7496..8956
                     /gene="cls"
                     /locus_tag="ACHKW5_004034"
     CDS             7496..8956
                     /gene="cls"
                     /locus_tag="ACHKW5_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO7513320.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8991..9320
                     /gene="yciU"
                     /locus_tag="ACHKW5_004035"
     CDS             8991..9320
                     /gene="yciU"
                     /locus_tag="ACHKW5_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO7513321.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9373..10377)
                     /gene="oppF"
                     /locus_tag="ACHKW5_004036"
     CDS             complement(9373..10377)
                     /gene="oppF"
                     /locus_tag="ACHKW5_004036"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO7513322.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10374..11387)
                     /gene="oppD"
                     /locus_tag="ACHKW5_004037"
     CDS             complement(10374..11387)
                     /gene="oppD"
                     /locus_tag="ACHKW5_004037"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO7513323.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11399..12307)
                     /gene="oppC"
                     /locus_tag="ACHKW5_004038"
     CDS             complement(11399..12307)
                     /gene="oppC"
                     /locus_tag="ACHKW5_004038"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO7513324.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12322..13242)
                     /gene="oppB"
                     /locus_tag="ACHKW5_004039"
     CDS             complement(12322..13242)
                     /gene="oppB"
                     /locus_tag="ACHKW5_004039"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO7513325.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13328..14959)
                     /gene="oppA"
                     /locus_tag="ACHKW5_004040"
     CDS             complement(13328..14959)
                     /gene="oppA"
                     /locus_tag="ACHKW5_004040"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO7513326.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEARRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3939 a         3747 c         3641 g         4076 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcttggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtcaggcgt gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccgccggtgc
     4801 ttcttttggt ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa atcctttgat
     5761 tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag
     5821 aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac
     5881 tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca cgacattctc
     5941 gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc
     6001 aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc cttgctggca
     6061 aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc
     6121 cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttacacc
     6181 ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac gctggtgttt
     6241 ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca
     6301 tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa
     6361 ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg
     6421 tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt
     6481 tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat ttttggtccg
     6541 cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa
     6601 gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct gcaactgaat
     6661 caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt
     6721 gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta
     6781 ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga taacgatgca
     6841 gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc
     6901 gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc
     6961 tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat
     7021 aatgatatgc tcgtttcaat gttgttgaac tcaggtcatg gtattttcag cgataacgat
     7081 gaacaagaaa cgaaagctga cagtaaagaa tcggcgcaaa aatagagttt aaagacggaa
     7141 gagtaaactt attgggcacc gcaatggtgc ccagtacatt tagatattgt agatcgcaaa
     7201 caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt
     7261 tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg
     7321 ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca ataacagaat
     7381 ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag
     7441 atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac
     7501 aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg
     7561 cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt
     7621 gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca
     7681 tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct
     7741 taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc
     7801 attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca
     7861 actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg
     7921 ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc
     7981 tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc tcgactccgc
     8041 cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga
     8101 agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg
     8161 ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat
     8221 ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc
     8281 gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat
     8341 tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca
     8401 ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat
     8461 tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgaccacgcc
     8521 ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc agcgcggggt
     8581 ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg
     8641 cgcattcttt acggaacttc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt
     8701 actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct
     8761 tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt
     8821 tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc
     8881 ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt
     8941 cagtccgttg ctgtaaaaca tcgccaacag aggttaaaca ggtagtgact atggatatgg
     9001 atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg
     9061 aactggctgc cgacaatctc gatccagctg atgttctgct gttcaatctt cagtttgaag
     9121 agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat gttgatttcg
     9181 atttgaaccc agactttttt gccgaagtgg tgattggtct ggcggacagt gaagatggtg
     9241 aaatcaacga tgttttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc
     9301 atatcatctg gcgggaataa ataatcgctt ttagcgtgaa aaagggctga caactgtcag
     9361 cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc
     9421 ctccagaaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac gggtacggaa
     9481 aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt
     9541 tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta
     9601 aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag
     9661 atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcgataaa
     9721 aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg
     9781 aattgacacg tccagcgccg aaaccggctc atcgcagata atcagcttcg gttcaagaat
     9841 aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat gcggatagcg
     9901 gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac
     9961 ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat
    10021 ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg
    10081 aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc aggcaacatg
    10141 accgtcggtc gctttaacca acccaatgat ggcgcgagca aaggtggact taccgcatcc
    10201 cgattcccct accacaccta atgtttcccc ttcatacagg cgaagagtga caccatcgac
    10261 ggctttgagc gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg
    10321 cactttaaga tcggcgattt caaggaggac ttttcttcct tcagttacag cattcataac
    10381 agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc ttccagcggc
    10441 ggtgcgctac tacaaatttc catcgcatgt ggacaacgtg gctggaacgg gcaacctttc
    10501 ggtaatcgca gcaagtttgg cggattaccc gggatggtca acattgtttc accttccgca
    10561 tcaagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag
    10621 acatcgcgcg cattgccata ttccatcgtg cgcccggcgt acattaccag cactttgtcg
    10681 cagatccccg ccaccacgcc aagatcgtgg gtgatcataa tgatggcggt attaaattcc
    10741 cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgcagta
    10801 gtgggttcat ccgcaatcag tagcttaggt cgacatagca atgccatcgc aatcatgact
    10861 cgctgacgca tgccgccaga aaattcgtgc gggtacattt tcatgcgttt acgcgcttcc
    10921 ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg
    10981 ttcttatgca gcatcagcac ttccattaac tgctcaccga cgcgcatata gggattcaac
    11041 gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca
    11101 tgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg
    11161 ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca
    11221 cctacaatac ccagcgtttc tccggcacgt agggaaaaat tcaaatcatt gaccgccgtg
    11281 acgtcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtgcgtca
    11341 gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt
    11401 aacgatcttt cgggtcgagg gcatcacgca agccatcgcc gataaagttg aaacaaaaca
    11461 gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt
    11521 tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca
    11581 ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca
    11641 ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac
    11701 cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt tgcccacgca
    11761 caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc agcaggatgt
    11821 tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag gagttgagga
    11881 tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac
    11941 cataaagtgt cccgacgacc acagccacca gtgccgcagc aacaccgacc atgagtgaga
    12001 tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt
    12061 agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg
    12121 caaactggga aagcatcggt gccaggatta caaataacgc gatcagcacc agcactatca
    12181 gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc cacaagctgc
    12241 gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta
    12301 acatcatcgc gagctccagt atcagtaacg aattttcggg tcgataaccg catatagcac
    12361 atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac
    12421 taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc
    12481 ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata
    12541 agagagcacg ggtaacagag caggttttaa tgcgtgacgt aaaatgatcc gccgcatagg
    12601 taaccctttc gcccgggcag tacgaataaa attggagtgt aatacttcaa tcatagagcc
    12661 acgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat
    12721 catgaattta agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc
    12781 aaatatcatg actaataatg gtgcaaccac aaaactgggg ataacaaccc cggtcattgc
    12841 cagccccatc acggtatagt cccatttggt gttttgtttt aatgcggcaa taacgccagc
    12901 actaacaccc agtattaccg caaggaaaaa tgctgcggct cccagtttgg cagaaacggg
    12961 aaaactggat gcaaccaagt cattgaccga ataatcttta tatttaaacg atggaccgaa
    13021 atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag
    13081 atgatatttc gcttcgatat tggccatcac ttctggcggt aaagtacgtt cgccggtaaa
    13141 agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gttataagaa taaatagcgt
    13201 cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct
    13261 gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt
    13321 attgccatta gtgcttcaca atgtacatat tccgggtata ggtattatcc agcggatctt
    13381 tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag
    13441 gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgcc
    13501 gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat
    13561 aatgcgcggt attcatcgag ctgttcgaaa gcatggtgtt caggaaggaa gttggttcgt
    13621 tgtagtcagc acaccagcct gcacgggcca catcaaaagt accctggtga cgggtgtcga
    13681 ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca
    13741 atgaagaggc agcaatcgcc agctttttat gcagatcgga ggtgttatac aacaggttga
    13801 tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt
    13861 tacgtttttc ctggctccag ccaaaccatt ccggctgagt caattttgcg ccatcagtat
    13921 acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga
    13981 tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt
    14041 tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg
    14101 ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttataa gtcatgtcga
    14161 tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta
    14221 cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc
    14281 gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct
    14341 gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt
    14401 gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat
    14461 catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa
    14521 taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg ttcggatcaa
    14581 cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcca tcagaccatt
    14641 tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag
    14701 cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc
    14761 ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac tgaacttctg
    14821 aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag
    14881 ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct actaaacttc
    14941 tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca
    15001 gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta
    15061 ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca gacgatcacc
    15121 ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt
    15181 taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc ggaaagtacc
    15241 aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc tcttttattg
    15301 accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca attcaatcaa
    15361 atagagattg gctaagcatg tctatctttt ctgattacag ttc
//
LOCUS       DAZFYC010000119        15087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFYC010000119 DAZFYC010000000
VERSION     DAZFYC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.119"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(109..519)
                     /locus_tag="ACHKW5_004041"
     CDS             complement(109..519)
                     /locus_tag="ACHKW5_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO7513327.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(818..2200)
                     /locus_tag="ACHKW5_004042"
     CDS             complement(818..2200)
                     /locus_tag="ACHKW5_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO7513328.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVTAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2210..3160)
                     /locus_tag="ACHKW5_004043"
     CDS             complement(2210..3160)
                     /locus_tag="ACHKW5_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO7513329.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(3303..4721)
                     /locus_tag="ACHKW5_004044"
     CDS             complement(3303..4721)
                     /locus_tag="ACHKW5_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7513330.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(4721..6268)
                     /gene="fdrA"
                     /locus_tag="ACHKW5_004045"
     CDS             complement(4721..6268)
                     /gene="fdrA"
                     /locus_tag="ACHKW5_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7513331.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(6258..7121)
                     /locus_tag="ACHKW5_004046"
     CDS             complement(6258..7121)
                     /locus_tag="ACHKW5_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7513332.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(7161..7766)
                     /locus_tag="ACHKW5_004047"
     CDS             complement(7161..7766)
                     /locus_tag="ACHKW5_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO7513333.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8024..8521
                     /locus_tag="ACHKW5_004048"
     CDS             8024..8521
                     /locus_tag="ACHKW5_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7513334.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGSKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            8613..9545
                     /locus_tag="ACHKW5_004049"
     CDS             8613..9545
                     /locus_tag="ACHKW5_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7513335.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9587..10675)
                     /locus_tag="ACHKW5_004050"
     CDS             complement(9587..10675)
                     /locus_tag="ACHKW5_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7513336.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(10817..14800)
                     /gene="ehaA"
                     /locus_tag="ACHKW5_004051"
     CDS             complement(10817..14800)
                     /gene="ehaA"
                     /locus_tag="ACHKW5_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO7513337.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKDQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANRGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGNQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         3495 a         4082 c         3550 g         3960 t
ORIGIN      
        1 cgggcagcaa caataagtgt gacattcatc acgtaaaaaa atagcaaaat aaacaaaatt
       61 agcaatattt tatgtgtgtg agtttatttc gcgtagataa atagttatct acacaacgtc
      121 aataagggca tattcttcaa atataacgtc catttctggt gaatagcttc cttctgcctg
      181 gaaaaaacga cggtgttcat tacgccaata ttcaagactg aggtcacctt caccttcctt
      241 acgggcaagc tccacggtga catcagaaaa gcgcagtaaa tgcaggccaa tcactctgat
      301 aacacataca ggctcaccac ggctattttc tacaatctta tactcaccaa tcatcggaaa
      361 tgcgttgtct tcaatacatc ccgccagcga accacaggaa gctgttttaa caccagtcgc
      421 aatcaacgtt gcgaggtgat cggccaacgc tgctgaatcg ccaaaagtac aaaaggaagc
      481 atggggatat ttttgtgaga atttttcttt tatggtcatg gcaatatttt tgcgattacg
      541 gaaaaacaaa tcacatctta attactgttg gtaaccatca gcaagagtat cattggtttt
      601 tcgatactga atctttttta tccgaagcag gtcacattta tggcctgata aatacctttg
      661 ttaaaactca cttttgtgta tttctctcaa ataatcccct ggaaattatg ccgcgagatt
      721 ataaagttca ggatcattca ttagcaaacc attcccgccc cactccctgg ggcttaaatc
      781 tcttacaatt caccgttgca ggcgtttcaa atgcgcatca acctgccaca ctcccccaca
      841 ccagttgccc tttatggaac gttgcggttc tcggcgagat acgcgccacc gcctcggcgg
      901 aacaggaggc gtccaccagc acaaagctgg catcatcctg cgcttttggc catacacgct
      961 cgcctttttc attcagcggc aatacatcgc cagtggctag aaacagcgaa cgggagaggt
     1021 tctgttcgtt aggacgaata tagagctgcg cgtagagatt ggctttttcc agcatgtcgc
     1081 ccagaccata aggcgaccag tggtcgataa cgctgtcagt gccagtcatc acttttacgc
     1141 ctttgtcgtg caactgtttg agcggcatat gcagcgtgcc aatcggcacc gtcgaggcga
     1201 tagaaatttg ctgcgccgcc atccgattcg ccagcgcatc gacctgttgt tcgttgagcg
     1261 tagccagcgc aaaggcgtgg ctgatggtca gcttaccttt cagttgcggc gttttctcta
     1321 ccgtttcaac catataattg atggctgtca cgcccgacgg agtggtttcg tgcaggtgaa
     1381 tatcgacacc tttgtcgtag tccagcgcaa tctgaaacat ggtgtcgagg gatttttcca
     1441 tcgcgccatc gacactggtc gggtccagcc cgccgacgta atgcgcaccc gcctgcatcg
     1501 cttcgcgcat taagggttcc gatttcgaca gcagcaaacc gtgctgcggg aaggcgacga
     1561 tttcacactc gaagcccgcc tgacgtcgcg ccagcaccgc ctgcaaattc tgcagatttt
     1621 tcaggccgga aaccggttcg atattgcagt ggctgcgggc aatggtggtg cctttcgact
     1681 gcaatagatc gatcagtttt tctgcccgtt cctgcgtgta cggttgcagt tccggcagca
     1741 ttttctgctc aagcttgatc atgtcctgga tagtggtgcc tgccggacga ttgagcgagc
     1801 gccacggccc gccgtagaag gttttgtcga gatgaatatg catgtcgcga gtggtgggta
     1861 gcatcaactt accgccagcg tcatagtgcg gcagcgtggc gtccggatgc tgcttgttct
     1921 cgcgcagggc gacaattttg ccgtcctgaa tctccacggt ctggcgcgcc gtgcgggtct
     1981 gcaccgccac accgttttcg tagtcaaaac cggtttccag cagcacgtta tcgagatagt
     2041 aatgcgggtc agtgattttc atggtgttgt tcgcttcgca gtttagggtg ccagctaccg
     2101 ccgcatgggc gagcggcaca gaggtaccaa acagcgcggc ggcggtgacc atcttaccgc
     2161 tctggctcag aaattcacgg cggctattgc tttctttcat cctacgtcct tacgttttaa
     2221 taatatgggt gcccgtttca ccgcgcagcg ccgcaggcag gcattccggc gtggtgataa
     2281 tcacttcttt gccgccctgc tccaggaacg tcaggctggc gatgattttt ggcaacatgc
     2341 tgccgggcgg aaaatgccct tcctgcatat agcgagtcat ggtggcaata tccacccgat
     2401 ccagcgcctg ctgctgcggt ttaccaaagt ggatacagac tttttcgacg ccggtggtga
     2461 tcaccagaat gtcggcgtga atttcacggg ccagcagcgc ggtagagaga tctttgtcaa
     2521 taaccgcgtc cacgctttgg taatcccccg cttcagtacg cactaccgga attccaccgc
     2581 cgcccgcgcc aattacgaca aaaccttgtt ggatcagcgc cttaatggcg ggtgcttcga
     2641 caatacgttt cggttccggc gaggcgacca cgcggcgata gccccgtccg gcatcttcaa
     2701 caaaacacca gtcagggttt gccttttgta gtttgtcacg ctggctttca ctgaagaatg
     2761 caccaatggg cttggtggga tgggcaaaac ctggatcgtt tttatccact tccacctgag
     2821 ttaccacggt gacggctttt ttctcgccgt gacgcgccag ccggttattc agcgcctgtt
     2881 ggatcagata gccgatgccg ccctgcgtat ctgccacaca gttcgccagc ggcgttaagg
     2941 gcagcccttc gcgctcgtgg gcaatctccg cacggcgtaa atccagcccg acctgcggcc
     3001 cgttaccgtg ggtcagcaca atgtcgtaat cggaagccag catttccagc accgtatcgg
     3061 ccacggcttt caccgcctcc gcctgatgct caatcgactg gctggcgtta tctttgataa
     3121 tgctgttgcc accaatggca acgaccacaa gctctttcat gttgttatgt ccgctacaga
     3181 gggtgattag gttatgacca acgccagact gcctgatgcg ctacgcttat caggcctaca
     3241 attctcgggc catccgtagg ccggataagg cgttcacacc gcatccggca taaacatgta
     3301 agtcagacgc cgtaacgttc gcaccagccg agaatggctt tctcgaagca ggcgaatggt
     3361 ggatgcacaa tgcccgcgcc aatcatgccg acgcccgcat ctttatgggc gatggcggtg
     3421 ttgatcaccg gcagaatgcc gctgctaccc acgcgggtga tgtcgattgc cgacggcacg
     3481 cccataaaac cgagcagcgg aatggtgacg ttggggtttt cgccaagggt gatttcgcgc
     3541 atctgacggg agaaatcgat agcctcttcc accgtgccgc ccaccagtgc gacgattgca
     3601 ggcgcggtcg ccatggcaaa cccgccaata ccgtaggttt ctgtgatggc gctgtcgccg
     3661 atatccagcc ccgaatcttc cggcttataa ccagcaaaca tcgggccgat gacctgctgc
     3721 gctgggccgg taaaccactg ccccggcagg ccgctgaccc gcaggccgaa ctcgacgccg
     3781 ttacgcgcca tggtggtgac cacggtgctg tattcgatgc cgtgcgccgc atccatcgcc
     3841 gctttacaca tcgccatcca cgtcgggccg gagaagtagt cgctgctggc gacaaactcg
     3901 aacacttcgc gctgttgctc gacggaatag cctgcctgaa tgatccccgg cgtcagtgcc
     3961 tgaatcagca gcgtcgtccc ggcgttattg cggttatggc actcatcgcc catatgcagc
     4021 gcctgcgcca gcattaagcg cagatcgatc tcaccgataa ttttcatcgc gtcgcgcagc
     4081 attggcccct gcacatcgcg catccagttg aggcggtcga tcacgctctg atcgttggca
     4141 cccatacgca aaatcttcgc catctgctcg ctcatattgg tataagcgat gttgccgtag
     4201 gttttgtttt tcacgatgtg cataaacatc gaggccgagg taacgcccgc catcgatccc
     4261 acgcagtcgt gttcgtggca cggcgagaag gtgatctccc cggaggccgc cagttcagtc
     4321 gcctcatcga gatctttcgc cagcccttcg aacaccaacg ccccggtgac cgcgcctttc
     4381 atcgcgccgc acattttttc ccaggtgatc ggcggcccgg cgtgaagaat ggttttcggc
     4441 gtcatgcccg gcaccacgtt aatcgcctga tcaaaaccaa tcagcaccgg atgcgactgg
     4501 ataatgcgct ccagcgcctg ctggttagcg gcggcgattt tgtctgccag cggcgaatcg
     4561 gcaatgttat ccagcgcctg caccacctgc atattgccct gccccggcgg cgtccagtcg
     4621 agttgagtca cttctacatg ctgctttttc aggtcatcgc taaacatggc gataccgacg
     4681 ttaataacgt tcaacggttg gctaaacagt gactggctca tcaggcttcc tcccctttgc
     4741 agataaattc acgcgccagc aatccggtat tggtgctgct gctcgccaga atcactccgg
     4801 catcaagcag catctggctt tgttgttcta atgatggcgt atcgagatcg gtgcccagca
     4861 cataggcgag aatgatcaac tcgcgtcctt cggcggcggc gatcgctttc gcttctttga
     4921 tcgcctcgat ggtggagccg accggatctt catgcgatcc aaatccgagc acaaaatcca
     4981 tcacgatcac cgccacttct ggatcgcgcg cctcttcgat caagcgactg atgcggttgg
     5041 tggggtcaat cattggatgc ggcttgccgt tggtgaagtc gtcatcgcca aagtcgagga
     5101 aggtgtgttt gatgctacgg ttgatatctt gcaggcggaa ttccggatcg ggctgaatgt
     5161 tgctgtagac atcgccgtgt ttttccatca ccgcgaacat ggtttcgtca cacagcgtgc
     5221 cgccacagaa caggccacga atgtacttct gctgcggttg caggcgagca cgtacgtcag
     5281 caatcaacgg ctggttcagc gtatgcaggt cgagatgttc ctgtttcacg ccggagagca
     5341 tcaccgcttt caatgccgcc tctttgctgc cgcgggcaaa ctgtagcccc tgctcatcca
     5401 ctggcgtttc gccacgaccg aggaaacaga ccaccaccgg cttgcggcag gcgcgcgcgc
     5461 gttccagcac tttgcgggcc accgcaggcg cgggcggttt ggagataagc acaatgattt
     5521 cagtttgcgg atcgttttcc agcatgccga tggcgtcgag catcatcagg ccgccgattt
     5581 tctcgctcag gtcgcgcccg ccggtgccaa tcagttgcga aacgccgccg ccaaattcat
     5641 gaatgcggac gctcagctcc tggctgccgg tgccggatgc gccaacaata ccgatgttgc
     5701 cgcgacgcac ggcgttacca aaacagagcg ccgcgccgtt aataatcgcc gtgccacagt
     5761 ctggccccat catcagcagc cctttttcgt gggccagttg cttgagcgcc aattcatctt
     5821 caactgagac gttatcggaa aacagcatca cgttgagatc gttttgcagc gcctgacgcg
     5881 cttcgcgagc ggcaaacaga ccgttgaccg aaatcaccgc caggttactt tccgggatat
     5941 gctttttggc gctggcaata gtggcgtaac gcgcctcatg cgagccgctt tgcgctttgg
     6001 tgttgaacag ttcttcaatc tccaccagca actgttcgtt gtccgcgccc gctttgccgt
     6061 tgatgacaat catcaggtcg ccgtttttcg cctgttccag ctccggcgtc agcagtccta
     6121 aatttttcag tacacctttg ttcatttcgg tcgccatcgc cacaaatgcc tgctcgacgc
     6181 cgtcgagttt atttgcacgc gtggagatag acatcagcga gacagaatca aaataggtat
     6241 tcggtttaat gactatttta actgacataa ttccctccgt aggtttggct cagcgcacaa
     6301 ccatcggcca tgccatacag catgtcgcag ccggaactgg agccaatatg tttaattttt
     6361 tctattgcct gagttgcgtt cccagggatg tcataaataa tgttgtgaat aaaacgatga
     6421 atattttccc ggcaacgttg ttgtaatgcg gcttccagcg ttatagcact taataatgtg
     6481 gtgttatttc tgccgcgctg cataccgaga taaaattcct ctttgtattt ttccgacgga
     6541 tgcccgggaa taaataaaat aagcgccaga cctgttaaat aatcgtcggc tgatggcgta
     6601 aggccaatcc ctaacccaat catactggcg accgctgcgg cgatattttg tttttcgttt
     6661 actgcctgaa taactgcccg tcgtttaata tgtagttctt tatttaacgc ctgataaaac
     6721 ggattatcgc cgcgataatt aaacagggtt tcattttccc ccaggtgctg atgaataatg
     6781 tcgtgccact gttgccaggc gataaggtga aaattcacag cggtcatttg caaggttggg
     6841 gacagccaac gccgacaacg actcatctct atatgaagat gttgaccaac cgtaatacct
     6901 tgatcgtcaa actgaacctt atcaccatgc cggaacagat catcaaagtg agtgagtgcc
     6961 aaccgacaac tgtttggcgc attgtcgtac tcttcacaaa gcagcgtcag caactgctgg
     7021 cgagccggga gtgcgatatt taccgctctg gcaaagacct gttcaacctg tccttgcccc
     7081 cgctgcgcca gaaaatccgc atccgcagtt agcgcccaca cacaccgtct ttctggtgtg
     7141 ttgatagcaa gcctcccggt ttatgcacct gcggcaatca gtaactgcgc aatctcttca
     7201 aagccccgtt cccgcgccag ttccagtggc gttttgccat atttatcggt cagatgcggg
     7261 ctggcaccgt gttccagcaa taactgcaca atcgcctgct gtttaatacc accatcatta
     7321 agcacaatcg cttccagtag cggcgtccag cctacatggt tggtctggtt aacgttaatc
     7381 tccgtatgcg ccaaaagctc ttttacaata cttaaatggc ctttttcaca ggcaggcgtc
     7441 aggccgacac cgccaaaacg ggttacgcaa ttaagatcgg gtttagccgg taaaataatt
     7501 cgtagtagcg ttaaatcatc gttcagacag ctaattaaaa aaggatttaa acaggtatga
     7561 tcttgcttat taatatccgc tccggcatca attaatgcct gaacgcaagc atattgctga
     7621 tataaacttg ccagcgtaat tgccgttttc ccctgacgat cgcaggtatt aatatcgaca
     7681 cccagcgcaa ggcaggtttt tactttatcg atatcaccct gttgtgcagc caataaatag
     7741 tcggcaggta gattttttat actcattcac attcccctgg tttttgtggc tgttttcgat
     7801 gaattgagaa tagcaacgat aatctgataa aagaacccgc ttaaatttaa ttcgctgtat
     7861 ttcgcctttt tgttgaatga tgttcaacaa tcttttcgca gtgtgcggta gtgcaatatt
     7921 tgtttctttg acccgatacc ctttcactca atcaggtttt atttctctat agtctgcaca
     7981 tgtaagagta gctttcgtcc atgtctaacc agaggggaac aagatgaacg gactcacggc
     8041 aacgggtgtc accgtcggta tttgtgcagg gctttggcag ttagtgtctt cacatgtcgg
     8101 gctctctcag ggatgggaat tattaggaac cattggcttc gtcgcctttt gcagttttta
     8161 tgccgcaggc ggcagtaaat cggggtttat cagaagtctg gcggttaatt attccgggat
     8221 ggtgtgggca tttttcgccg cgctggctgc cggttggctg gcctctgtaa gtggactgtc
     8281 cgcgttctgg gcgagcgtaa ttactaccgt acccttctcg gcggtagtgg tctggcaagg
     8341 gcgtttctgg ttactgtcct ttattcccgg tggctttctc ggcatgacgc tgttttttgc
     8401 cagcgggatg aactggacgg tgacgttact gggttttctg gcgggtaact gcgtggggat
     8461 aatttcagaa tatggtggac aaaaattaag cgaagcgacg actaaacgcg acgggtatta
     8521 aaagttattc aaaaatgatt atctatcttt aaccgatacc tctacagtca tagctggaga
     8581 aattcatagt agataatggc gagaatggtt taatgaactc aatttttacc gaagagaatc
     8641 tgttggcatt tactaccgct gcacgttttg gcagcttcag taaagccgca gaagagttgg
     8701 gtttaaccac ttccgccatt agctacacca tcaagcgtat ggagacgggg ctggatgtgg
     8761 tgctgttcac tcgcagtacc cgcagcattg agttaacgga gtccgggcgt tattttttcc
     8821 gcaaagctac cgacctgctg aatgattttt atgccatcaa acgcagtatc gatactattt
     8881 cgcagggcat agaagcgcgt gtgcgcattt gtattaatca gctgctttat acgcccaaac
     8941 acaccgcacg cttgttacag gtgctgaaaa agcagtttcc tacctgccag attaccgtca
     9001 ccaccgaagt gtataacggc gtctgggatg cgattattaa taatcaggcc aatatcgcca
     9061 tcggcgcacc cgatacacta ctggacggcg gcggtattga ttacactgaa attggcgcga
     9121 ttcgctgggc ttttgctatc gcgccggatc acccgttagc ctttgtgccg gaacccatcg
     9181 ccgaaagcca actgcgtctc taccctaata ttatggtgga ggacaccgcg catacgatta
     9241 ataaaaaagt gggctggttg ctgcacgggc aggagtcgat tctggtgcca gatttcaaca
     9301 ccaaatgtca gtgccagatt ttaggtgaag gaattggttt tttgccggat tacatggtcc
     9361 gtgaggcgat gacgcaatcc ctgctggtta cccgacaaat ccataatccg cgccaggatt
     9421 cgcgcatgtt actggcgact cagcattctg ctacgggtca ggtgacgcag tggattaaaa
     9481 aacaatttgc gccaaatggc atattaacgg ggatctatca ggatttactg catcgggaga
     9541 attaatcgct gccaacctcc cgatgcgctg tgaatagcgg tttacatcaa ccacctgctt
     9601 tcattaccca ttcagagata aatttattac ccggtactgg cggagagtac aaatagccct
     9661 gcaaaaagtg aacgccttta ccgatcatta aatccgcctg ctcctgggtt tctacccctt
     9721 ccgccacgat actcagacca ggcttacgcg ctagttcgac aatattgtcc acaatatgac
     9781 cggagatttc gtcgacactc gccatttgca caaatgactt atcgatctta ataaaatcga
     9841 ccgggaacgc ctgcaagtaa cgataggtcg cataacccgt accaaagtca tccagcgcaa
     9901 aggtaatgtt gtgctgatga aggctgtcaa atatcgctct ggcttctggc gttaccggaa
     9961 tagggttacg ttccgttagc tcgagaacca gcttgatttt atcgttacct aatttcttaa
    10021 ccaggttcag acactctttt tcaaatcccg ctgccaaaaa acaacccgcc gagacgttga
    10081 tgccaatatg gaaattgtcc ggcagcaaat gttttaccgg catcagaata tccgcagtct
    10141 gtttcatcag ttggcgggtc attatgacaa taagaccgga tgactccgcc agaggaataa
    10201 actgatccgg tgggataatt cccgtttgtg gatgttccca gcggacaagt acctcacagc
    10261 ccgtcagtac cccagtctgc gcgcagaaaa ccggttggat ccacggtttg aattcgtggt
    10321 tttccaacgc cagactgatg gcttcaggcg tgacgatatg gtgacaacgg ttatcaggga
    10381 tataaaaatt atttttattt cccgtgccac tgatgacttg cggatgatat tgaaaaaaga
    10441 tatcccgcca gaaggtaata tcactctgga tgcctagttt ttcatacagc tgcttctttt
    10501 gatgtgaaat tgtctttgca ctgcgatttc tgtactgtga gatttctgta accgacattc
    10561 cctccattag cagcttgagt aaaagccgtt ctttttcgga cacgatacca ggcaacgtca
    10621 aattcaccgt tgccccgaac tcttccaaaa aaacactcaa atcatgcgaa gccattgaac
    10681 acaccttgat taaatcttgt gcgccagcta aatctgcgag tcttcactct gactgataaa
    10741 gctggctgca agaaaatgac acagagagcc gaattatctc ccgttcggct ctctctcctt
    10801 ttactcatta ttcagatcag aattgccact taatgccaac cattgcagag gtgtcattat
    10861 atcccctatc ggcaacctga acgcccacat tcccccagac attcaggttg gcatttagct
    10921 gtccttccac cccggttttt atctcagcaa tatttcgggc tccatcctga gtgacggaca
    10981 cgccatccat actggtgctg aaatccttac tgttatgcag ccagttcact tctacaaacg
    11041 gctggaactc gcgggattta ccgtcatcca ttttatggtg gctcttcagc caggttttta
    11101 cgccaagtcg ggtttgaaca ttgccatcac cgttgctatg aacgagggtt ccgttgcttt
    11161 cgcggtgctt atcggctttg actcccatcc aggtaacctg tgcttgcggc tgaacatacc
    11221 attcattacg cgttccctgg ttgccattaa attcagctaa tttgtgtttg tatccagctt
    11281 ccagtgaagc ggtaaatcct tttgatttat aggattcact ttgtaagtca tcccctttca
    11341 ctgtgttatc aaaccagctg tactgcgccc aactgtcgag atacgcgcca ttacgcgact
    11401 cgtcatcggc ataccaggtg gcatagaggc cggtgctata tccgttcaca ctcgcttttg
    11461 cacgataacc ggttcgcgag gaaatggttt tgctgtcgct gttgccatat cccgccatga
    11521 ccccaacatg ccagctgtcg ctgccgtttt ggctccactg cgcgacatcg cctcccagtt
    11581 gcagaacata gcgattgctt tgggttttca gctggccgct gccatcacgc catttattat
    11641 gaccaccttc atggcgcatc cacatagtgg tttgtttctg ctcacccgtc accatgtcgg
    11701 tatagtacgt attacccaga cgctcatgca gacgcgtggt gaacattgta ttcgctgctg
    11761 caaggttcgc aatgtagcta cccgcctccg gtcgcaggtc attatccaca ttcagatccg
    11821 gacccggcgt aggtgtcggg ttagggttcg gctctggatt cgggtttggc tccggattcg
    11881 gcatcgggtc tggctccggc tgcagttcag gagaatcact gccgctggtc agataccagt
    11941 taccacgatt tgccccttgt ccacgcgcga gagtgtagtc atacgccccc gcaacgatac
    12001 gaccagcctg aacaaattcg ccctcagact taccgtctac atggataagt tcgataccgt
    12061 taagggtttt cgcacctgta ccgccagcgt tattcaccgt aacggcagtc gtgccgctag
    12121 tatcgccctc gacaaccaat ttatcggtta ctgagtcatc gccattcagt accgtattca
    12181 ttactagttg accgttatta ccgtggtagt tccccttcac cactaacaca ttgccagctt
    12241 gctgtcctga cattccggtc atcagcgtac cgttgttggt cagattgccg cctagggtgt
    12301 aattcacaga cgcagccgct cttttttcag cgctattatt caacgccagg gtaccgttgt
    12361 tctcaacgtt tgcgctaccc aatccctgcg catgacttac caggacatta gccgaattag
    12421 caatgttcac attcgccgtc aggttgctgt tatcgcccgc gatgtccagc gtgccgccct
    12481 gaatattcag ctctccgctc cccgttaacg aaccggttga agtgcccccg ttggtaaccg
    12541 tcagactgcc gccatttaag ctcagcagtg aatcggcgct gccattgagc gtgcccacgg
    12601 tctggctgtg accattcata tccagttcag tctctgccgc cagacgcagt tcatgggtat
    12661 tacccaacac gttatcgtta ttcaacaaaa gcgtcccact gcgcagatcg gtaacaccgg
    12721 tatagtcgtt gtctttgtta gaaagcgata cggtctgatc cttctggctg ctgaatgcca
    12781 ggtcaccgct gccggtaatc tttgcgctga gatcggcggc attctcgctt ttaccgttag
    12841 agctcagcac cagagcatcg ctatcggtag cgtgtaaatc aagctgggtc aggccgtaac
    12901 cgatatacag accgtcattg tttaccccac tcgtcagacg atagtcgtaa gtgccgacgg
    12961 ccgctttttg cccattctgc tgaatatcac gttgggcact gctggagagg atttgcccat
    13021 ttttatcctg caattgcaga ttgcccgcat cgcccagaac ttccgcacct tgcgcatcaa
    13081 ccaacttaat ggtgctgtta ccatcatcga cttcagtgag cgataacgca gagtcaatat
    13141 cacgagatac tgagcttaca acgtcactgt cagaaacctg aatcacaccc tcgccgcgca
    13201 aatccagcgt tttactaacg ttgaccgtcc cttctgtcat ctgtgcacct gctgtcaggg
    13261 caccaaaatc aacggttccg ccggagaatc ccaaagtgct aatggagtga gcgcccttac
    13321 ccactgttac caggcttccc tgacccgcag tgatcccgcc tctctgcaga aactcagcat
    13381 tcttttccag gttaaatgtc gtgttctgga acgccagtga accctggaac cagtctgtat
    13441 aagcaaactc atcgaaggag aattcgtgat tccccatgtt tatttcaaca atacctctac
    13501 cttgaagatc gttatcaaat acccaatctt tattggcatt gatttgcagt ttgccatcaa
    13561 catccaggtc cgcacggccg atgttcttgt cttcagaaat aaccgcgaca gcaccagaag
    13621 cgacatcaat atatccgctg aatgcatagt tattgccggt cagtgccact gtcccactgt
    13681 cgatacttat tgtaccattg ccggaaatct tattggcaaa ggtcatgcta tcgtcagcct
    13741 tcaggtgtaa tttcccgcgg ttgttgatct ctgctgtacc tatatttttg gtgatcccta
    13801 aagcgccatt ttgctcaatt ttgactttac cactaaaggc agagttatca ccggttaagt
    13861 aagtcgtgcc attgagcgca ttgatatagc ccaggccctc gctgccactg acaatatccg
    13921 cggaaatagt cgcgttttta tccgtgtggt ttaaggtgat tttcttatcg gatgtctttg
    13981 cccacacaaa ctcaattttt tcggcatcaa taatccctgc agccttcgca gcacttcctt
    14041 cctgtgcacc taacgctaac tcagagttgg tgcttaaact aattttcggt gcggatattt
    14101 tacctccatc tgatacgact aatgttgtat cagaacgttg gtcgtaataa cccaggctaa
    14161 tatgttcacg agcattaact tcaccaccgt tattaaccga caggaactga ttaccgccat
    14221 atccgccgta aacgcccata aagaaggtcc ctgcatttac ctgactacct tcgccgctta
    14281 cattcccctg cgtataagtc agacgactgg tgcccttaac atgaattttg ccgccattgg
    14341 agacattaaa atagccttga tcatgtctca tgcctgagtt tgactcatac aaatcatcag
    14401 catataactc gctgccagca ttggagacat tcaggaaccc cgtacctttc agcaaaaagt
    14461 taagcgggcc attgattgtt acctttcctc cggtaatatc caacccacca ccagaagcga
    14521 gagtcagacg cggacgggaa gtatctgtcg tattgattac cagattggca ttgtcagtca
    14581 ccgtaatacg gtaatcgatg ctctggttct ctttgccaaa atcaatagct ttatcctggt
    14641 ttacgatgac atcatcggcg aataccagag atgaatataa acttatcgcg ctaattaaaa
    14701 cagcccggga cttttttcct tttactctgc tccccagctc cgagcagacg ctccaacagt
    14761 tacgggacct gttccacttt agccgatata ttttattcat cacaatctcc agcaatacat
    14821 ccaaataaaa ccaagattgc ataaacagca acgcattaaa tgccattctg gttactgttc
    14881 atcgcatata aaaattcaaa aattttgcat agcattggca accacatatc tgagtatgta
    14941 gttcagaggg taataaaata gcaagttatt acttaaaact tattttaagt taacactctt
    15001 aactttatca ttacattcct cccagtctct aatacctgcg cattatatgt atccaggaac
    15061 tcaaatcaac tcaaaatata aacaaaa
//
LOCUS       DAZFYC010000120        14953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFYC010000120 DAZFYC010000000
VERSION     DAZFYC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.120"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1440
                     /locus_tag="ACHKW5_004052"
     CDS             <1..1440
                     /locus_tag="ACHKW5_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO7513338.1"
                     /translation="TLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINS
                     LIIDYDNEDVQSYWEDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVL
                     EDQKDKNFIAKMIAEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQD
                     DHENDGDRIWQTLNDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWL
                     NEILKSDSPDIRCRNIDFVFGNLSEELYQRLKNNNTLDEFIKSVFDSYSNEYANSGVA
                     ALLQYCSSKMDLPSNNDTYHEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCV
                     SAACDLVPTQGNDPHHVRLSPHRLIKVLELFNASQSKALPFAEYSKYIYVMHKNQRKY
                     LSIFEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQL
                     RTGYAERYQAIASQYSSRIGVDYVSMMLP"
     gene            complement(1512..1928)
                     /locus_tag="ACHKW5_004053"
     CDS             complement(1512..1928)
                     /locus_tag="ACHKW5_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513339.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(1967..3196)
                     /locus_tag="ACHKW5_004054"
     CDS             complement(1967..3196)
                     /locus_tag="ACHKW5_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO7513340.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(3522..4694)
                     /locus_tag="ACHKW5_004055"
     CDS             complement(3522..4694)
                     /locus_tag="ACHKW5_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7513341.1"
                     /translation="MGEKKQISLSLPRGITIRQYKNCSTLVVTFTYKGVLCREPLPRL
                     EANAKGIKYAERLLGEIKNQISDDSFDFSKYFPNSRKLALFGMVKKNKSVRDYLDEYL
                     EICKNRKLSPSTINGYEKCVSALSELHKLHVVDLTPAILKKWISKRTTKLKTIRNNLS
                     FLRSAVDEAVTDGLITINPVKLVSASRYHAVNHTANTDDYEVDPFTPAEATAIYNACL
                     YQEWRNLFCFAFNTGLRSSELCALRWSDIDFIGHTVHVQVASVVGVIKGTKTKAGTRK
                     VELNNEAIMALQAQKSFTMMRSDFVFSDPKTRKPWANSAAIRKKAWLPTLKKAGVRYR
                     NPYQTRHTFATRHISQGANLFWLAGQMGHKGPEMLFRHYGSYLSEYDGKTAISEVI"
     gene            complement(4649..4864)
                     /locus_tag="ACHKW5_004056"
     CDS             complement(4649..4864)
                     /locus_tag="ACHKW5_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7513342.1"
                     /translation="MALNIDCVPITTWCRDMGETLDAVNKRLQRGVWKEGVQVLNVEG
                     SKERWIDLTEVSKWARKNKYPYHSQEE"
     gene            complement(4883..5017)
                     /locus_tag="ACHKW5_004057"
     CDS             complement(4883..5017)
                     /locus_tag="ACHKW5_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513343.1"
                     /translation="MHTAFEFWVRKTFGNRYDLPRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            complement(5021..5263)
                     /locus_tag="ACHKW5_004058"
     CDS             complement(5021..5263)
                     /locus_tag="ACHKW5_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7513344.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVHIG
                     EFNSLFEHIDRQEYRKILAGTEQEMHLKKLRAMQRR"
     gene            complement(5351..5596)
                     /locus_tag="ACHKW5_004059"
                     /pseudo
     CDS             complement(5351..5596)
                     /locus_tag="ACHKW5_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(5713..6069)
                     /locus_tag="ACHKW5_004060"
     CDS             complement(5713..6069)
                     /locus_tag="ACHKW5_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513345.1"
                     /translation="MTTITDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEPIA
                     WECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLAYSDYA
                     EGWNDCREAMLQGDKS"
     gene            complement(6403..6581)
                     /locus_tag="ACHKW5_004061"
                     /pseudo
     CDS             complement(6403..6581)
                     /locus_tag="ACHKW5_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6583..7530)
                     /locus_tag="ACHKW5_004062"
     CDS             complement(6583..7530)
                     /locus_tag="ACHKW5_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7513346.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAVKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            complement(7527..7694)
                     /locus_tag="ACHKW5_004063"
     CDS             complement(7527..7694)
                     /locus_tag="ACHKW5_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO7513347.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRKGGKQ"
     gene            complement(7705..7998)
                     /locus_tag="ACHKW5_004064"
     CDS             complement(7705..7998)
                     /locus_tag="ACHKW5_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO7513348.1"
                     /translation="MPAPLYGADDVRRCSGNSVSEVLDKFRKNYARIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(8022..8405)
                     /locus_tag="ACHKW5_004065"
     CDS             complement(8022..8405)
                     /locus_tag="ACHKW5_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513349.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGSIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNGPQQQSSNHQNTPVYDDSIPF"
     gene            complement(8405..9010)
                     /locus_tag="ACHKW5_004066"
     CDS             complement(8405..9010)
                     /locus_tag="ACHKW5_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO7513350.1"
                     /translation="MSKEFYAKLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(9021..9191)
                     /locus_tag="ACHKW5_004067"
     CDS             complement(9021..9191)
                     /locus_tag="ACHKW5_004067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513351.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQNGDREGVRVFLNAAKTEV
                     LNKRYFLGPCPF"
     gene            complement(9267..9419)
                     /gene="kil"
                     /locus_tag="ACHKW5_004068"
     CDS             complement(9267..9419)
                     /gene="kil"
                     /locus_tag="ACHKW5_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO7513352.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(9404..9535)
                     /locus_tag="ACHKW5_004069"
     CDS             complement(9404..9535)
                     /locus_tag="ACHKW5_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO7513353.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(9560..10528)
                     /locus_tag="ACHKW5_004070"
     CDS             complement(9560..10528)
                     /locus_tag="ACHKW5_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HFO7513354.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKFGASEDVAKNIITAIVKGEVFATKITY"
     gene            10835..11317
                     /locus_tag="ACHKW5_004071"
     CDS             10835..11317
                     /locus_tag="ACHKW5_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006641566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HFO7513355.1"
                     /translation="MYMIVIWVGLLLLSPDNWSEYVNERIGIPHVWHVFVFALAFSLA
                     INVHRLSAIASARYKRFKLRKRIKMQNDKVRSVIQNLTEEQSMVLCAALNEGRKYVVT
                     SKQFPYISELIELGVLNKTFSRWNGKHILFPIEDIYWTELVASYDPYNIEIKPRPISK
                     "
     gene            complement(11582..11774)
                     /locus_tag="ACHKW5_004072"
                     /pseudo
     CDS             complement(11582..11774)
                     /locus_tag="ACHKW5_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
     gene            complement(12106..12540)
                     /locus_tag="ACHKW5_004073"
     CDS             complement(12106..12540)
                     /locus_tag="ACHKW5_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HFO7513356.1"
                     /translation="MRNRIMPGVYIVIIPYVIVSICYLLFRHYIPGVSFSAHRDGLGA
                     TLSSYAGTMIAILIAALTFLIGSRTRRLAKIREYGYMTSVVIVYALSFVELGALFFCG
                     LLLLSSISGYMIPTIAIGIASASFIHICILVFQLYNLTREQE"
     gene            complement(12556..13395)
                     /locus_tag="ACHKW5_004074"
     CDS             complement(12556..13395)
                     /locus_tag="ACHKW5_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rexA"
                     /protein_id="HFO7513357.1"
                     /translation="MKNGFYATYRSKNKGKDKRSINLSVFLNSLLADNHHLQVGSNYL
                     YIHKIDGKTFLFTKTNDKSLVQKINRSKASVEDIKNSLADDESLGFPSFLFVEGDTIG
                     FARTVFGPTTSDLTDFLIGKGMSLSSGERVQIEPLMRGTTKDDVMHMHFIGRTTVKVE
                     AKLPVFGDILKVLGATDIEGELFDSLDIVIKPKFKRDIKKVAKDIIFNPSPQFSDISL
                     RAKDEAGDILTEHYLSEKGHLSAPLNKVTNAEIAEEMAYCYARMKSDILECFKRQVGK
                     VKD"
     gene            complement(13508..14221)
                     /locus_tag="ACHKW5_004075"
     CDS             complement(13508..14221)
                     /locus_tag="ACHKW5_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7513358.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            14322..14522
                     /locus_tag="ACHKW5_004076"
     CDS             14322..14522
                     /locus_tag="ACHKW5_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7513359.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            14641..14934
                     /locus_tag="ACHKW5_004077"
     CDS             14641..14934
                     /locus_tag="ACHKW5_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO7513360.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
BASE COUNT         3860 a         3341 c         3051 g         4701 t
ORIGIN      
        1 acacttaaac tattacaaga tttgaaagac tccgatcatt taaatatgat tgtaatatat
       61 actagagaga atttagaaac ggtttggatg cagatatcat cgactctcaa aggtgctctg
      121 gatatcaaca gcttgatcat tgactacgat aatgaagatg tccaaagtta ttgggaagac
      181 gttgtattac cgaacttaaa tgataatggt aataaagctc tcacaagaga tgaaacaata
      241 gcctatatta aagacagtaa gccttgtaga agaattaaaa gattaataca tgatgatgct
      301 gtgttggagg atcaaaagga taaaaacttc attgcaaaaa tgattgcaga atatgctgtg
      361 tctagaaatg caattatttc tagcaacaca tctggcaatg tcattcgggg tgatgaaagc
      421 ggagtaaaat ggattcaatg tggtaatatc tttgtctccc tatttcataa ggttcaagat
      481 gatcatgaaa acgatggaga taggatttgg caaactctca atgattctct cattgaatgg
      541 aaaccatctt attatcagtt aataaaatct gaaattcaga atgcaatcga agctgaggct
      601 ttatcttttg taaatcattt ggctaacgat cattacggtc aagctgcgtg gttaaatgag
      661 atattaaaat cagactcgcc tgatattaga tgtagaaata ttgactttgt atttggtaat
      721 ttatcagaag agctttatca aagacttaaa aataataata cgctggatga atttatcaaa
      781 agtgtttttg atagctattc aaatgaatac gctaacagcg gagttgctgc attgctccaa
      841 tattgctctt caaaaatgga tctgccatca aataatgata cttatcacga aatgtatcat
      901 gctttaaata tgaatttgtc ttcaaagaat tttgaagatg gtcatatttc tactggcact
      961 attttctttg atacagagtc gaacaaatgg tatttatgtg tatctgcggc atgtgatttg
     1021 gttcctactc agggtaacga ccctcaccat gtaagattaa gtccgcacag gctcattaaa
     1081 gttctggagc tttttaacgc cagtcagagt aaagcattgc catttgctga atattcgaaa
     1141 tatatatatg taatgcataa aaatcaaaga aaatatctct ctattttcga aggggataaa
     1201 acgcttcctg ttgttgatta tatggtggtg ttgaatcatg gaacaacagt tgatggcgaa
     1261 gaaaaaaata ttatttctgc cgtgttttta agtaatatgg atggcaacgt gcaaaatgtt
     1321 cctgtccgac tcaaacttaa atctcaactg agaactggtt atgcagaaag atatcaggct
     1381 atagcgtctc agtatagctc aaggattggt gtggattatg tatcaatgat gctaccataa
     1441 ttattatatt ttaggcgtgg tgattttttt tcgccatgcc tattttatat ttatcatcac
     1501 aatgatgttg tttatttttg tttaagtctt ctaagcttca tgcattctaa tgagaataat
     1561 aatagagttg tgctgtatat aaagccagag ttagttaata actttaaatt taatgcttca
     1621 ggattttcac tgagaaagtg aaaggcataa actaagtata cagctaaagt agccgttcca
     1681 attgttggtc caatatcgcc ttgatcatga ggttcaaggt ttacattgaa cttgaagatt
     1741 agccattcaa atccccaagt aaaaaaaaca actaatatca tggctgcgaa taatttcgcg
     1801 atgttatctg ctgatggatt catcagaaca gattgtttct gaaaaaattc acaaagtgag
     1861 agtccgaaaa aaataaaaaa aagtggtctg gaactaaatt tttcaaaaac tttgaatagg
     1921 gtttccattt tctttacctt caggattata ttagctattc tgatacttag gctaccagag
     1981 catttgatct ttgagcttga acgtaatcac tccaccattg catcaaactc tgtcgctcta
     2041 tcagatattc tgcacgattg tatgctgcga taatttcatc ttttttcgag tgggcaagcg
     2101 ctgcctcaag aacttcagct ctgaatttac cagactcctc tgccgctgtt cgtgcaatag
     2161 aacgcatacc gtgagctaca agctcgcctc cgaaccccat tcggatgata gctgcgttgg
     2221 ctgtttgttc atgcatatga ttaagaggcg cttttatgct ggggaaaacc cattctctat
     2281 gcccacttat tgatttcatt aattcaagga tgcgcaaagc ttctttactc aaaggaactt
     2341 tgtgaagctt tttcattttc atgaaatcag caggaatgtt ccaaatgctg ttggttgtat
     2401 caatatcaga ccaccttgcg cgaacggctt cacccggacg aacccatgtc aacaattgcc
     2461 attcaattag catacgtgtt tccaaccgga ttgacgcatt cgtcaaagat tccataaacc
     2521 ttggcaattc gcttggggga agggcaggca tattttgctt ttttggttta ctgaatcttt
     2581 gaccaaggtt gtcagccggg ttgaactcaa taagttcttc agtagctgcc caccggaaga
     2641 tttcattcag acgtgaaatg atacggcgta gagtttccaa tacgcctcgt tgctcaatag
     2701 gatcaaggtg ttgttttaag agcttaggtc ggatctcatt gatagggaca ttacccagac
     2761 cgggaaagac atttctctct aagctgcgcc agatgtctgc tgcatggtct tgtgagatac
     2821 ctgatgtctt taccttctca tctaaccatt tccgcgctac ggcttggaga gtgtgctcag
     2881 tagcactctt taatgccttc gccttatcgt tgttatggat ttggggatca acaccatttg
     2941 ccagaaagga gagatattca tcacgtaagg ctctggctct tgcaagggta aggtgaggat
     3001 atgtcccaag gctcattttg gttcttttct tgctcactgg tactgcatac ctgaaatacc
     3061 aatttttctt gcctcctttc gccaaaggag cgattcgtag aatcagacca tcaccgtcaa
     3121 acaagttgat ttctttatcg gctggcttgg tgcttttgat ttcagtgtca gtgagcttct
     3181 tagcgatttt tgccattttg ggaccctcgg tttttggacc cttcttagtg ggtcccattc
     3241 agggtgccat aactcgtagt tctcagcaat tctcactgga cgacaataga cgtaaaaaag
     3301 cccgcagagc ttgtgctgtg tgggcttagt agacttcatt gaacttcaaa caactaaaaa
     3361 gtggtggagc tggcgggagt tgaacccaaa cacccaagtt acataacgat atgaaaaaca
     3421 aaagatttca tattatttct agatgttttt atgtatttca ggtgtaagtt ggagtctatt
     3481 ctctgtccta ccttagtaca tattttgaaa tatttaagcg gctagattac ttctgagatc
     3541 gcagtcttgc catcatattc tgataaatat gaaccatagt gtctaaacag catttctggt
     3601 cctttgtgtc ccatttgacc ggcaagccag aataaatttg ccccctggct gatatgccgt
     3661 gtagcgaatg tatgtctagt ctgatatgga ttgcggtaac gaactccggc ctttttaaga
     3721 gtcgggagcc aggctttctt acgaatcgca gctgaattag cccacggctt gcgtgttttt
     3781 ggatcactga atacaaaatc acttctcatc atcgtaaatg atttctgggc ctgtaatgcc
     3841 attatagctt cattgtttaa ttcaactttt ctggtaccag cttttgtttt ggttcctttt
     3901 atcacaccaa cgacagaagc gacctgtacg tgaacggtat gcccaataaa atcaatatca
     3961 gaccaacgca atgcacataa ttctgagctt cgtaggcctg tattgaatgc gaaacaaaaa
     4021 agatttctcc attcctgata aagacaggcg ttgtagatag ctgttgcctc tgctggtgtg
     4081 aatggatcca cttcataatc atcagtattg gctgtatggt taaccgcatg gtatctactt
     4141 gcactaacca gttttacagg atttatcgtt ataagtccgt cagtgaccgc ttcatctact
     4201 gcacttctta aaaatgatag attgtttctt atggtcttaa gttttgttgt tctttttgag
     4261 atccattttt ttaatattgc tggtgtcagg tcaacaacat gtagcttatg tagttcagat
     4321 aatgcagata cgcatttttc atatccattt attgttgaag gtgataactt tctgtttttg
     4381 caaatttcca gatactcatc taggtaatct ctcacacttt tatttttctt taccattcca
     4441 aataatgcca gcttcctaga gtttggaaag tattttgaaa aatcgaatga atcatcactg
     4501 atttggtttt ttatttctcc tagaagccgt tcagcatact taatcccctt tgcgttagct
     4561 tccagcctgg gtaagggttc tctgcaaaga acccctttat atgtgaaggt tacaactaat
     4621 gtagagcaat tcttgtattg acgaattgtt attcctcttg ggagtgataa ggatatttgt
     4681 tttttctcgc ccattttgaa acctcagtta aatcaatcca tcgttctttg gagccttcca
     4741 catttaaaac ctgaacccct tccttccata ctccacgttg taaccgtttg ttaacggcat
     4801 ccagcgtttc tcccatatct cgacaccatg tcgttatagg tacgcaatca atgttgagag
     4861 ccatagttac ctcataaaac gttcatccac ggcagtggca ccacacgtca aacattcgtt
     4921 tcacaacttc acggcagtag aagccgtcaa catcacgggg caggtcgtag cgattgccga
     4981 atgtcttgcg aacccagaac tcaaaagccg tatgcatgta tcacctccgt tgcattgcgc
     5041 gtaatttttt caggtgcatt tcctgctcag tgcccgccag aattttgcgg tactcctggc
     5101 ggtcaatatg ttcgaacagg ctgttgaact caccaatgtg tacccgcccg gagcgtccgt
     5161 ccatgcgccg aaagaatact gagtgctgag tactgcgagt aatcaccaca gggtatccgg
     5221 ctctgtccgt gtatatctga ccacgttgaa tcagagcgaa cattccttta tccccagcgg
     5281 aaaagcgaat acagaataaa tgccaccgct attgcaactc ctactgcggt gaatgcttca
     5341 ggccaattca tcatttcacc tcctgcggcg gttctggtag ctgcatccag tgtgttacct
     5401 gctcaacacc attgcccggc ttaatcgtta catctccacg ccggaatgta cttccggtat
     5461 agcgtgcaga gcatattagc ggctcaacca gagagctatc gaaattcacc gaaataagca
     5521 cgctctgatt cttttccggc attcgctcac tacagcttat ccaaccatcc ggagttaccg
     5581 gagagttgcc agccttgcgc atggcaatct ccatgatttc aaccatatct cctggtggaa
     5641 ttttacaatg ctgaccaata tgcctctgct gcctggcata ttcgaggatg tgctccagct
     5701 tgatacgatt aatcatgatt tatctccctg aagcatggct tcgcggcagt cgttccagcc
     5761 ttcagcataa tcactatacg caagaggcca accgtttctg tattcacgcg gcaacttatc
     5821 aggcactacc agcgctggcg gcgcggagaa tagtggttta ggtgatattt ccgcacgttt
     5881 tgcgtatgct tcaactgtgt cagggttaaa caggattatg ttttcaccgc attcccacgc
     5941 tatcggttct gcttccagcg atgccagtgc aatttcataa gcacggcgct caatattgtc
     6001 tcgaacgtcc aagctgccta tgcgctcttt gatttcttta atcagttctt tgtcggtaat
     6061 agtggtcatg ccgcgtttcc ttctttctta ttaacaatta caccgtcata tatttcatta
     6121 aggtgccctc tcaactccat gcgccttaat gcagacaaca tgtaatcgca ttcaacctgc
     6181 ttatttccag taaatggctt atcgtcagga ttaccccaac agcaattacc cttgggccac
     6241 ccatgtactt tccgtactct tccgttaaca acgtgaagta atccccagcc aggtggtaaa
     6301 tcctcaattg aaataattcc cggctcacta ataaagaatc gccagtcgcc cattccaaga
     6361 gacggatttt tacgaaaacg cttttttcta tctgccaaca agtcagcacg agaacatttc
     6421 gcctctatca ggcatgatgc tgaatttctg aatcccatag catctggctg ttctccggta
     6481 ctggttacag ctataaagcg gtcatgaaaa caaaccttga acccgttgcg cttaaggaac
     6541 ttgtacgcaa tctgacagag ttcgcggtgt gttaacgcca tatcactctc ctttgatgcg
     6601 aatgccagca agccagtttc ttatgccgat atattcagcg ttcctgaaac cgctttttac
     6661 atatataaat ggcaagcgaa gattgtgacc attgactgcc aggtagtctt tacaaccctg
     6721 ttcggtgaaa cagcaggtaa cgaattcatc aatatctttc acagcaacgc gccgccattt
     6781 ttctggtggc tctcgaaagt tttcatgaag tagctcgaga cgacgactat ggcgtttatt
     6841 ggcttcattg ccatcttcgt caacccagac aatccggtca tggtcataat cagcatcaac
     6901 aacaatttcg cgcttttgat acacacaaaa catgggatct gacgttattc gattgtcctg
     6961 tgttcgaata ttttcaccga tgatgccaaa cgaatctggt gcagattttg tctgcatctc
     7021 ttcgatacgt tcagccatcg cagcacactc ttcaaagttg cttaatgctt ttcgctccca
     7081 ttcggcgcat tgtttttcca gttctgctat gcgcttactt ccatccgcga ttactccctc
     7141 gtaatattca cgctgctcgt tgagttttga ttttactgct tcaagctcaa cgcgcagctt
     7201 ccctaccgta agcgcaatat cctcgttctc ctggtcgcgg cgtttgatgt attgctggtt
     7261 tctttcccgt tcatccagaa gcgccagcgc aacatttgga ttaaaggcag caataaattc
     7321 agcgtttgcg taagcctgag catctgtttc aaccaggcag ttaacatgac attctgcaat
     7381 cacgccaccg ggttctcctt tccatttttg acaaacaaaa actcctgtta aattgccgtg
     7441 ctggttaaca gatgtatgcc ctacgatgta gcttccttta gttgctttct ctgccttttc
     7501 acgcagtgcc tgataattaa tttcgctcac tgcttgcctc ctttacgcca catcgcattc
     7561 agatatttgt tttgattcac tgatggaaaa gagtttctct taagcaattc ctctctcgat
     7621 ggcattggct ttacgcgttg gcgaataatc atttctgccg gaagaatgcc gggattgtat
     7681 gcaagtcctc tcatggtaaa ttcctcagtc attactgata gcgccatagc gtgagcggta
     7741 attacgcagg cgcgggtcaa tttcagggaa gtgggtatat gtggctttgc ggaatggtcg
     7801 gattgatgtc tggtaaattc gctcgcgctc ttctttctct gcaagccata tacagtggcg
     7861 aaattccttt tcctctttcg tttcctgcgg tagcgacatt atccgggcgt agttttttct
     7921 gaatttatcc agcacctccg atacggaatt gccggaacag cggcgcacgt cgtccgcacc
     7981 atacagaggc gctggcataa ttaaatcctt attgttctaa atcagaatgg gatggaatcg
     8041 tcgtaaacag gagtgttctg atggttacta ctttgctgct gcgggccatt tcctgaagct
     8101 gcaaatccaa tctttgcatt cagtaattca agagtgattg attgaccatt ttgcccctga
     8161 taaacatcaa ccctgatgtt ttctccggta atttctacaa tgctaccttc aacaagaaca
     8221 ctacggtagt aatccgcttg cgctcccggc ttggcaaata caacggcgct gtagtttgtc
     8281 cattctttct tttttgtctg gcgatcgtaa tactgaacgc cagcacggat gttgaatccg
     8341 atattttccc cggcctgaaa ctctcttgcg ggtttgttta gtcttactgt aatcgaatgt
     8401 gccattaagc agccgctcct tctaattcgt ctcgtctgat gttgtaaacg tcctgcgctt
     8461 tgtgctgctc cggtgtgcct tcgagcatct tccacgcttt ggcgaacgcc tgtttaagct
     8521 cttctacggt gtttttctgc attgctgcgt cagtgaatgc ttttagaacc tgttcaggtg
     8581 taggtgatgg ttttgattgc tttgctgctg cgttctgctg atgtttatgc tcgtcggtat
     8641 ctgcatcttt cgcatcatca atgccgaaca aaccattgag gcaatacttg cgtgcataag
     8701 agcttgtggc tcccgtaact tgtgcagaat ccattccttt cttgctttct tcctctcgtg
     8761 caagagcggt tgccgtatga ctgttttcgc catcggtaat agttgccgtg gctttcacgt
     8821 aataccgatc accaatcaac acaacttcat cgctgattga taaaaacaga ccgttcagta
     8881 acggcttaac gccttcaaga atgtcttcgc agcttctgta tttatattta ccgaatgagt
     8941 tgtactgatt ctttggcgca ttcaagttct cctgaatagc tgccagtttt gcgtaaaatt
     9001 ctttgctcat atgtttgttc tcagaatgga cacggcccaa ggaaataacg cttatttaat
     9061 acttcagtct ttgccgcatt taaaaatacg cgaacacctt cacgatctcc attctggcga
     9121 tacattaacg cctgctgcgt gtacatgcgt ctctgtaact tgctctcctt cactgtggtt
     9181 gcaagtgaca tgaatatctc cttcgttacc gattaaatct ttcatctgac gaatgaattc
     9241 ttcgtctgac cagttatctg taaaactcat ttcctgcgat accacggaag gttgatagct
     9301 gatttcattg ctttatttgc ttcaagccac atttttgaat caccaataaa tctggctatt
     9361 actgctttgt tttgtgccgc acgaagcatc tggtgattaa tggctatttc attgcgcata
     9421 ataagacctc aactcttttc catccgtcac gtaatttacg ggtgattcgt tcaagtaaag
     9481 attcatttaa ttggaaggca cccatgcgag cgcctcccgc gattgcgtaa atcatgggtg
     9541 gttccttatg ttggttttat tagtaggtta tttttgttgc gaatacttcg ccttttacga
     9601 tggctgttat gatattttta gcaacatctt ctgatgcacc aaacttgata aggtcagcaa
     9661 gtattttgtt atttacttct ttccggtgag ctttatcctt tgctctacgc tcttcttcgt
     9721 ccttgattct tttttcttct gctattctgg cttgctcttt tgcttcagcc tcgcgccgga
     9781 ttcgttcagc ctcctcctgt gcttttctac gttctgcttc aattgccgcc tgcttttctc
     9841 tttcggctcg ttctgctgcc tcttttgctt cgcgctgtgc tcgttgctcg gcttcaatgc
     9901 gttcacgctc tgcacgttcc gctgcggcct tagcttctgc ttctcgcctt gctgctgctt
     9961 caatttcggc ttttgccttt gcttcggctt ctgctctggc tttctcttca gcttctcttt
    10021 ttaagcgttc ttcatgctct cgcttttcct gctccgcttt gagtcttgcc tcttctcttt
    10081 ggcggtcaaa ttcgcgatcc atcaaaatcg ctatttcatg gtcagactca atttgctttg
    10141 cgagagcttc agctgctgcc ttagcttctt cttcggcttt aattcgcgcc tgttcttcct
    10201 cataatcagt aagaggctgg cgtgccttgg ctttcagttc atcaaggcga tcgcgcactg
    10261 tcttgcggtt ggcatcaatt agctttggaa tttccttcag ttcagcaaca aggtctttgc
    10321 caagaccatc gagatatgtt ttcgtctgcg caactttata cgccagagaa gcgatctcct
    10381 ttctgccctt tgccgttgtg atatcaggca caaaggacat aacttcacgt tcaacctttt
    10441 gaaggatttc ttcaatctgg tcggcagact gaaatacagt cattgcattt gctttttcaa
    10501 taacaactaa atctgttact tcactcatat atcctccgtc aaaaaaatcg ccctcacact
    10561 ggagggcaaa gaagatttcc aataatcaga acaagtcggc tcctgtttag ttacgagcga
    10621 cattgctcag tgtattcact cgttggaatg aatacacagt gcagtgttta ttagtatgcc
    10681 tgtcttttaa ccacatcagg ctcggtggtt ctcgtgtacc cctacagcga gaaatcggat
    10741 aaactattac aacccctaca gtttgatgag tatagaaatg gatccactcg ttattctcgg
    10801 acgagtgttc agtaatgaac ctctggagag aaccatgtat atgatcgtta tctgggttgg
    10861 acttctgctt ttaagcccag ataactggtc tgaatatgtt aatgagagaa tcggtattcc
    10921 tcatgtgtgg catgttttcg tctttgctct tgcattttcg ctagcaatta atgtgcatcg
    10981 attatcagct attgccagcg ccagatataa gcgatttaag ctaagaaaac gcattaagat
    11041 gcaaaacgat aaagtgcgat cagtaattca aaaccttaca gaagagcaat ctatggtttt
    11101 gtgcgcagcc cttaatgaag gcaggaagta tgtggttaca tcaaaacaat tcccatacat
    11161 tagtgagttg attgagcttg gtgtgttgaa caaaactttt tcccgatgga atgggaagca
    11221 tatattattc cctattgagg atatttactg gactgaatta gttgccagct atgatccata
    11281 taatattgag ataaagccaa ggccaatatc taagtaacta gataagagga atcgattttc
    11341 ccttaatttt ctggcgtcca ctgcatgtta tgccgcattc gccaggcttg ctgtaccatg
    11401 tgcgctgatt cttgcgctca atacgttgca ggttgctttc aatctgtttg tggtattcag
    11461 ccagcactgt aaggtctatc ggatttagtg cgctttctac tcgtgatttc ggtttgcgat
    11521 tcagcgagag aatagggcgg ttaactggtt ttgcgcttac cccaaccaac aggggatttg
    11581 ctgctttcca ttgagcctgt ttctctgcgc gacgttcgcg gcggcgtgtt tgtgcatcca
    11641 tctggattct cctgtcagtt agctttggtg gtgtgtggca gttgtagtcc tgaacgaaaa
    11701 ccccccgcga ttggcacatt ggcagctaat ccggaatcgc acttccggcc aatgcttcgt
    11761 ttcgtatcac acaccccaaa gccttctgct ttgaatgctg cccttcttca gggcttaatt
    11821 tttaagagcg tcaccttcat ggtggtcagt gcgtcctgct gatgcgctca gtattaccgc
    11881 cagtggtatt tatgtcaaca ccgccagaga taatttatca ccgcagatgg ttatctgtat
    11941 gttttttata tgaatttatt ttttgcaggg gggcattgtt tggtaggtga gagatctgaa
    12001 ttgctatgtt tagtgagttg tatctattta tttttcaata aatacaattg gttatgtgtt
    12061 ttgggggcga tcgtgaggca aagaaaaccc ggcgctgagg ccgggttatt cttgttctct
    12121 ggtcaaatta tatagttgga aaacaaggat gcatatatga atgaacgatg cagaggcaat
    12181 gccgatggcg atagtgggta tcatgtagcc gcttatgctg gaaagaagca ataacccgca
    12241 gaaaaacaaa gctccaagct caacaaaact aagggcatag acaataacta ccgatgtcat
    12301 atacccatac tctctaatct tggccagtcg gcgcgttctg cttccgatta gaaacgtcaa
    12361 ggcagcaatc aggattgcaa tcatggttcc tgcatatgat gacaatgtcg ccccaagacc
    12421 atctctatga gctgaaaaag aaacaccagg aatgtagtgg cggaaaagga gatagcaaat
    12481 gcttacgata acgtaaggaa ttattactat gtaaacacca ggcatgattc tgttccgcat
    12541 aattactcct gataattaat ccttaacttt gcccacctgc cttttaaaac attccagtat
    12601 atcacttttc attcttgcgt agcaatatgc catctcttca gctatctcag cattggtgac
    12661 cttgttcaga ggcgctgaga gatggccttt ttctgataga taatgttctg ttaaaatatc
    12721 tccggcctca tcttttgccc gcaggctaat gtctgaaaat tgaggtgacg ggttaaaaat
    12781 aatatccttg gcaacctttt ttatatccct tttaaatttt ggcttaatga ctatatccaa
    12841 tgagtcaaaa agctcccctt caatatctgt tgcccctaag acctttaata tatcgccaaa
    12901 tacaggtagc ttggcttcta ccttcaccgt tgttcggccg atgaaatgca tatgcataac
    12961 atcgtctttg gtggttcccc tcatcagtgg ctctatctga acgcgctctc cactgcttaa
    13021 tgacattcct ttcccgatta aaaaatctgt cagatcggat gtggtcggcc cgaaaacagt
    13081 tctggcaaaa ccaatggtgt cgccttcaac aaacaaaaaa gatgggaatc ccaatgattc
    13141 gtcatctgcg aggctattct taatatcttc aactgaagct ttagagcgat ttatcttctg
    13201 aaccagactc ttgtcatttg ttttggtaaa gagaaaagtt tttccatcga ttttatgaat
    13261 atacaaataa ttggagccaa cctgcaggtg atgattatca gccagcagag aattaaggaa
    13321 aacagacagg tttattgagc gcttatcttt ccctttattt ttgctgcggt aagtcgcata
    13381 aaaaccattc ttcataattc aatccattta ctatgttatg ttctgagggg agtgaaaatt
    13441 cccctaattc gatgaagatt cttgctcaat tgttatcagc tatgcgccga ccagaacacc
    13501 ttgccgatca gccaaacgtc tcttcaggcc actgactagc gataactttc cccacaacgg
    13561 aacaactctc attgcatggg atcattgggt actgtgggtt tagtggttgt aaaaacacct
    13621 gaccgctatc cctgatcagt ttcttgaagg taaactcatc acccccaagt ctggctatgc
    13681 agaaatcacc tggctcaaca gcctgctcag ggtcaacgag aattaacatt ccgtcaggaa
    13741 agcttggctt ggagcctgtt ggtgcggtca tggaattacc ttcaacctca agccagaatg
    13801 cagaatcact ggcttttttg gttgtgctta cccatctctc cgcatcacct ttggtaaagg
    13861 ttctaagctc aggtgagaac atccctgcct gaacatgaga aaaaacaggg tactcatact
    13921 cacttctaag tgacggctgc atactaaccg cttcatacat ctcgtagatt tctctggcga
    13981 ttgaagggct aaattcttca acgctaactt tgagaatttt tgcaagcaat gcggcgttat
    14041 aagcatttaa tgcattgatg ccattaaata aagcaccaac gcctgactgc cccatcccca
    14101 tcttgtctgc gacagattcc tgggataagc caagttcatt tttctttttt tcataaattg
    14161 ctttaaggcg acgtgcgtcc tcaagctgct cttgtgttaa tggtttcttt tttgtgctca
    14221 tacgttaaat ctatcaccgc aagggataaa tatctaacac cgtgcgtgtt gactatttta
    14281 cctctggcgg tgataatggt tgcatgtact aaggaggttg tatggaacaa cgcataaccc
    14341 tgaaagatta tgcaatgcgc tttgggcaaa ccaagacagc taaagatctc ggcgtatatc
    14401 aaagcgcgat caacaaggcc attcatgcag gccgaaagat ttttttaact ataaacgctg
    14461 atggaagcgt ttatgcggaa gaggtaaagc ccttcccgag taacaaaaaa acaacagcat
    14521 aaataacccc gctcttacac attccagccc tgaaaaaggg catcaaatta aaccacacct
    14581 atggtgtatg catttatttg catacattca atcaattgtt atctaaggaa atacttacat
    14641 atggttcgtg caaacaaacg caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa
    14701 atcgcaatgc ttggaactga gaagacagcg gaagctgtgg gcgttgataa gtcgcagatc
    14761 agcaggtgga agagggactg gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg
    14821 ggggtcgttg acgacgacat ggctcgattg gcgcgacaag ttgctgcgat tctcaccaat
    14881 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
    14941 tactggatct atc
//
LOCUS       DAZFYC010000121        14821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFYC010000121 DAZFYC010000000
VERSION     DAZFYC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.121"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(87..794)
                     /gene="yqgA"
                     /locus_tag="ACHKW5_004078"
     CDS             complement(87..794)
                     /gene="yqgA"
                     /locus_tag="ACHKW5_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO7513361.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            947..1030
                     /gene="yqgH"
                     /locus_tag="ACHKW5_004079"
     CDS             947..1030
                     /gene="yqgH"
                     /locus_tag="ACHKW5_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO7513362.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            1192..3327
                     /gene="speC"
                     /locus_tag="ACHKW5_004080"
     CDS             1192..3327
                     /gene="speC"
                     /locus_tag="ACHKW5_004080"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO7513363.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCQLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="ACHKW5_004081"
     CDS             complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="ACHKW5_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO7513364.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="ACHKW5_004082"
     CDS             complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="ACHKW5_004082"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO7513365.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5978..6253)
                     /gene="yggX"
                     /locus_tag="ACHKW5_004083"
     CDS             complement(5978..6253)
                     /gene="yggX"
                     /locus_tag="ACHKW5_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO7513366.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ACHKW5_004084"
     CDS             complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ACHKW5_004084"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO7513367.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7494..8213
                     /gene="trmB"
                     /locus_tag="ACHKW5_004085"
     CDS             7494..8213
                     /gene="trmB"
                     /locus_tag="ACHKW5_004085"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO7513368.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQPGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8213..8539
                     /gene="yggL"
                     /locus_tag="ACHKW5_004086"
     CDS             8213..8539
                     /gene="yggL"
                     /locus_tag="ACHKW5_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO7513369.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8723..9442
                     /gene="yggN"
                     /locus_tag="ACHKW5_004087"
     CDS             8723..9442
                     /gene="yggN"
                     /locus_tag="ACHKW5_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO7513370.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9618..10664
                     /gene="ansB"
                     /locus_tag="ACHKW5_004088"
     CDS             9618..10664
                     /gene="ansB"
                     /locus_tag="ACHKW5_004088"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO7513371.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDLATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAQALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10781..11788
                     /gene="yggM"
                     /locus_tag="ACHKW5_004089"
     CDS             10781..11788
                     /gene="yggM"
                     /locus_tag="ACHKW5_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO7513372.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(11844..13145)
                     /locus_tag="ACHKW5_004090"
     CDS             complement(11844..13145)
                     /locus_tag="ACHKW5_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO7513373.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13145..13648)
                     /locus_tag="ACHKW5_004091"
     CDS             complement(13145..13648)
                     /locus_tag="ACHKW5_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO7513374.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13693..14679)
                     /locus_tag="ACHKW5_004092"
     CDS             complement(13693..14679)
                     /locus_tag="ACHKW5_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO7513375.1"
                     /translation="MKMRSFTRSLVCASLLTLVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETATKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3942 a         3535 c         3737 g         3607 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtgg tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagatccc ggtaccgctg gcgcaaaaca ggacaataat cgcgacataa
      481 ttctgaataa aagattcatg cgttggcttc ttacgtgagt gacgaaacag attttgtgct
      541 ttggcgactg ctgtattgac acctttctcc agcaaacaaa tttcgccaat tagagcgcca
      601 agtagcgtag ctaaaaccat cgctggaagg ttggcgcatt tcacaaccag taaaatacca
      661 atccccagcg atgccagacc aaaaatagat gtcatggaga tgcggatacg ttccggtaag
      721 cgttggctga gcagtgcgcc gagaacgcca cccagtaaga ctgcactagc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttaac tgtattgttt gattttctta ttatgaactt
      841 tttgccttgt tgatggcttg tctggtcgca aagtcctggc ttgcacgctt tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccacctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt tccctttcta tgttactgct
     1021 catgcggtga agttaacgca cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggcggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt ccccgaaacg ggtttgcgct tatgaaatca
     1201 atgaatattg ccgccagtag tgaactggta tcccgacttt cttctcatcg tcgcgtggtg
     1261 gcgttgggag atactgattt tacggacgtc gcggcagtcg tcattaccgc tgcggatagt
     1321 cgcagtggca ttcttgcgtt gcttaagcgc accggttttc atctaccggt gtttttgtat
     1381 tccgaacatg ctgttgaatt acctgcgggc gttacggcgg taatcaacgg caacgagcag
     1441 cagtggctgg agctggaatc cgcagcctgt cagtatgaag agaatttgct gccaccgttt
     1501 tatgacacgc tgacgcagta cgttgagatg ggcaacagca cctttgcttg ccctggacat
     1561 caacatggtg cgttttttaa aaagcatcct gccggacgcc atttttacga tttctttggt
     1621 gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa aattgggcga tctgcttatt
     1681 catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca aagtctttca tgccgataaa
     1741 acctattttg tgctgaacgg cacatcggca gcgaataaag tggtgacgaa tgcgctgtta
     1801 acgcgtggcg atctggtgct cttcgaccgt aacaaccata agtcgaatca tcacggcgcg
     1861 ctgattcagg cgggggcgac gccggtctat ctggaagctt cacgcaaccc gtttggtttc
     1921 attggcggta ttgatgcgca ctgttttaat gaagagtatc tgcgccagca aattcgcgac
     1981 gttgcgccag aaaaagccga gctgccgcgc ccgtttcgcc tggcgattat tcagctggga
     2041 acctatgacg gaactgtcta taacgcccgt caggtgatcg ataccgttgg gcatctgtgt
     2101 gattacattc tgtttgattc cgcgtgggtc ggttacgaac agtttatccc gatgatggcg
     2161 gatagctcgc cgctgctatt agaacttaac gaaaacgatc cggggatttt tgtgacccag
     2221 tcggtgcaca aacagcaggc tggattctca cagacgtcgc agatccataa aaaagataac
     2281 catatccgcg gacaggcgcg tttttgcccg cataagcggt tgaataatgc ctttatgctc
     2341 catgcttcta ccagcccgtt ctacccgctg tttgccgcgc tggacgttaa tgccaaaatt
     2401 catgaagggg agagtgggcg tcggctgtgg gctgagtgcg ttgagttggg tattgagtcg
     2461 cgcaaggcta ttcttgcgcg ctgtcagctg ttccgcccgt ttatcccgcc cgttgttgat
     2521 ggcaaattgt ggcaggatta tccgacatct gtgttagcca gcgaccgccg ttttttcagt
     2581 tttgagccgg gggcgaagtg gcacggcttt gaaggatatg ctgcggatca gtattttgtt
     2641 gatccgtgca agctgttact cactacacca ggtatcaatg ccgaaaccgg cgaatatagc
     2701 gactttggcg ttccggcgac gattctggcg cactatctgc gtgagaacgg cattgtgccg
     2761 gagaagtgcg atctcaactc cattctgttc ttattaactc cggcggaaag ccacgagaag
     2821 ctggcacaac tggtagcgat gttggcgcag tttgaacagc atattgagga tgactcgccg
     2881 ctggctgagg tgttgccgag cgtttataac aaatatccgg tgcgctatcg cgactacacc
     2941 ctgcgccagt tgtgtcagga gatgcacgat ctgtatgtca gtttcgacgt caaagaccta
     3001 caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg tgatgaaccc ccaggatgcg
     3061 catagcgctt atattcgcgg tgaagtggag ttggtgcgga ttcgtgatgc cgaagggcga
     3121 attgcggcag aaggggcgtt gccttatcca cctggcgtgc tttgcgtggt acccggggaa
     3181 gtctggggtg gggcggttca acgttatttc cttgcgctgg aagaaggcgt gaatttgttg
     3241 ccgggatttt cgccggagtt gcaaggtgtc tatagcgaaa ccgatgcgga tggcatgaaa
     3301 cgattgtacg gttatgtgtt gaagtaagaa taaaaaaacg ggtcaccttc tggcgacccg
     3361 tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt
     3421 atatttgaac atcgccatga acgcgaaggc cagaaccacg gaataaccag cgaaaatcaa
     3481 ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct
     3541 cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc
     3601 actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa
     3661 gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg
     3721 agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat
     3781 cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt
     3841 ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacgatga agctgctggc
     3901 aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg
     3961 cagttccgcg cccagcagca ttgagaagat gaagaagatg gccatacgct tgtttttaaa
     4021 cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc
     4081 aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gagctgcgcc
     4141 aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat
     4201 gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc
     4261 ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc
     4321 aaacgagtta atcaatatca caaggaacat cgcctccggt gtcgtgacct gtgccgccat
     4381 gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca
     4441 tttgtcggcc acaatcccca gcagcgcagg cataaagacc gctgcgatac ccagtgagct
     4501 ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat aggaaccgag
     4561 ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag
     4621 cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat
     4681 ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg
     4741 gcaaataatt gcaaacattc ccaatttgca aaatgaaatt gtgatgtgga taacattttt
     4801 gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc
     4861 ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt
     4921 ctgataaaca tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt
     4981 cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg
     5041 ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc
     5101 ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga
     5161 tttcccctgc gagcggaaca catctttccc ggcagtatgt tgtaccacct gcattaatcc
     5221 cagcgcatcg gaacggctga ccgcatacgg gttaaaggaa gattcggtct gcataattgc
     5281 cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata
     5341 tttgtgcgca cgtttatcaa ggtggttcgg taccatgtta atggtgacgc tgtagatgat
     5401 acgcagcccg ttactgcggc tttgcagacg gtttttcagc agataatccg cgaagttgct
     5461 tgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa
     5521 aggttctttc gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc
     5581 gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc
     5641 ggcgatagtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata
     5701 ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt tgaatcccca
     5761 gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt
     5821 gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg
     5881 cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt
     5941 ttatgcaagt tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat
     6001 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctcaagca
     6061 gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgct
     6121 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct
     6181 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
     6241 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
     6301 gtaattgctg taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta
     6361 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
     6421 acataggcac aatatctaag tggaaatggc tgaaggtatg gcgaaacgcg gtcagctgcg
     6481 tcaggttatc ggcagcaatc tgccgttgcg ccagccaatg ccgcaaactt tcttcgtcgg
     6541 caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca
     6601 atacttcatc ttcgtgctgt aatagcaaaa agtagccggt gcgctccggc agcgtctgtt
     6661 tcggtttttt gcccggataa agcgcccagc tattgttggc ggcggcaata catccgtttt
     6721 gtagcggaca gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca cccaaatcca
     6781 tcatcgcctg attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc
     6841 ataatttatt ctcgacctct tttttcccag gccagccgct tacagcatag cagcgcgcca
     6901 gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
     6961 tcgctcctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg
     7021 ggaatttacc gccgtgtaag gtcgccactt gttgtgccgc tttatgcaga ttgcgcgcgc
     7081 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
     7141 gatcggtcac cgtcgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa
     7201 cctgagtttg ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt
     7261 gccagggcag cgtttttcgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
     7321 gcgacgcttg catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac
     7381 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
     7441 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
     7501 acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt
     7561 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc
     7621 cggtgatggg cgttgagttc agcgaagata tgctggattt ccccgcgctt tttggccgtg
     7681 aagcgccggt gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta
     7741 aagatcgccc tgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
     7801 gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg agtgatgtgt cacgatgcgg
     7861 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
     7921 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgtacaggtg ccgtttgccg
     7981 aactggtaaa aagcaaactg cagccggggg gcgtattcca tatggcgacc gactgggaac
     8041 cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag
     8101 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
     8161 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
     8221 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
     8281 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga
     8341 tgattttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct
     8401 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
     8461 gattgtgcgt aagtggctgg aagagcgcaa actggatgag gtacgcacca gcgaactttt
     8521 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc
     8581 gctacgctta tcaggcctgg aaagatgcac gatcgagtag gcgggataag gtgtttacgc
     8641 cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcaa atgctggcct
     8701 gattttttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
     8761 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
     8821 tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
     8881 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
     8941 gaaggattat caggctgaac tacgcagcac gctgccgtgg attgatgaag gcgcgaaaag
     9001 ccgcgtcgaa aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag
     9061 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg
     9121 cattatcgaa acgcgcagcg atggcctgac gtttcactat aaagccattg atcaggttcg
     9181 cgccgaaggc cagcaattag tgaatcaggc aatgggtgga attttacagg acagcattaa
     9241 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg
     9301 aagcctgggg gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
     9361 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
     9421 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
     9481 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
     9541 tgtaaattgt ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgttcac
     9601 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
     9661 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
     9721 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
     9781 aaaatctggt taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg
     9841 tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa
     9901 ttaacaccga ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg
     9961 aagaaaccgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    10021 gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg
    10081 tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca
    10141 ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacctagcg accttcaagt
    10201 ctgttaacta cggtcctctg ggatacattc acaacggtaa gattgactac caacgtaccc
    10261 cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga
    10321 aagttggcat cgtttataac tacgctaacg catccgatct tccggctcaa gcactggtag
    10381 atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg
    10441 tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg
    10501 taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg
    10561 cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa
    10621 ccaaagatcc gcagcagatc cagcagatct tcaatcagta ctaatcacct cgccccggta
    10681 tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg
    10741 ataatcaccg gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc aatggattgt
    10801 tggcacggca ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac
    10861 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc
    10921 tatcacctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga
    10981 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct
    11041 cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg
    11101 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    11161 tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga
    11221 tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc
    11281 tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    11341 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa
    11401 aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt
    11461 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    11521 gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca
    11581 gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg taacgctcga
    11641 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    11701 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tggaaaaaag ataccgaaca
    11761 actgcggcgc aatggggtga ttaagtaagt ttgatatagt gcctttgccg gataatataa
    11821 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    11881 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatggcagc
    11941 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    12001 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    12061 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    12121 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    12181 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    12241 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    12301 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    12361 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    12421 gtaaaaatac cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag
    12481 gcatccagaa acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca
    12541 cggcgtttag caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc
    12601 aaatatcccg ccataaatag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca
    12661 attaacacgt ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg
    12721 gtagaaaatg ccggatcata accttgtttg gtctgaatgg gattcagtgt gccaccaacg
    12781 gcagcagctg ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg
    12841 ttcacatgac caagtgaacc aggaacccga ccaaccatta cctgggcgag gttaatcaaa
    12901 cgtatcgcaa taccgccgct gttcatcagc gttccggcaa agatgaaaaa ggggattgcc
    12961 aatagggcaa agttatccag tccattagcc agtcgctgcg aaacagtgat tgccgcgata
    13021 tcccagggaa acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg
    13081 ggcacaccaa taaagaccaa cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta
    13141 tccattaact ggcctcctgc gcatgttgtt tcagttgttg aatgctttga ttaataaaac
    13201 acaaggcata aaacatcatc accaatccac ttaatggcag aatgatatat acgtatccca
    13261 tcggtatttg catcgcggca gatacctgtg atgttgccag cgttttatca attaatttca
    13321 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat aagaacgtta atgactatac
    13381 taagaagtaa ttgcttgcgc ttattcaggg cgagagcaaa taaatcgata gagagatgtc
    13441 tctgcgcgcc gacggtatat gcagcaccta ataaccctac ccagatcatc aaaaatcgtg
    13501 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
    13561 ctacgcatac aaccagagca agcatgacgg cgacagaaaa cgcggcaatg acgcggtcta
    13621 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagagac
    13681 tggcccggaa tattattgag cagcgttgtc gaacttctct aacaaagcag cttgttttgg
    13741 atcttttttg aagtcgtcaa acagaggctg aactgcggca cggaatggtg ctttatccac
    13801 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
    13861 ccacaatttt tgttgataag cttcggattt cttcgttgct gtttcgagga tttgttgctg
    13921 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
    13981 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca cccaggaagg
    14041 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
    14101 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
    14161 ctgaacacga attttcagcc ctttaagatc ttctggctta gtaatcggtt ttttcgcata
    14221 gaaactgcgt gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
    14281 gtccataatt tctttaccaa cttcaccaaa aacaactttg ttgaagtgag cctgatcttt
    14341 aaataagaac ggcaaattat agatagcata aatattatca aaagactcca gatcactggc
    14401 ggaacctttg gtcatatcca gagcgccatt ttgcaacaac tccatagttt ctcgcgcatt
    14461 acccatctgg ctgctgggat aaatacggat caccatatta ccttttgaca gctgttttac
    14521 ctctttcgcc agctcttcaa aactctgatg tacaacatga cttctttcca ggttgtgtgc
    14581 taactttaac gtcacttttt ctgcggcgtt tacgccagtg ctaaccagag tcagcaagga
    14641 tgcgcagact aatgagcggg taaatgacct cattttcata aaaaaactcc ctgttgaagg
    14701 atggaaattg gttgatatct aattgtgctt atcagtgctt tgtgcgacaa attggttaac
    14761 ctgtctgaca acttgtctat gtttatcaca acttgaaata aagtcagaaa ttaaatatta
    14821 a
//
LOCUS       DAZFYC010000122        14616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFYC010000122 DAZFYC010000000
VERSION     DAZFYC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.122"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..245
                     /gene="yfhL"
                     /locus_tag="ACHKW5_004093"
     CDS             <1..245
                     /gene="yfhL"
                     /locus_tag="ACHKW5_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO7513376.1"
                     /translation="KRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGHYETPTC
                     QKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(439..519)
                     /gene="shoB"
                     /locus_tag="ACHKW5_004094"
     CDS             complement(439..519)
                     /gene="shoB"
                     /locus_tag="ACHKW5_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO7513377.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(939..1319)
                     /gene="acpS"
                     /locus_tag="ACHKW5_004095"
     CDS             complement(939..1319)
                     /gene="acpS"
                     /locus_tag="ACHKW5_004095"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO7513378.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(1319..2050)
                     /gene="pdxJ"
                     /locus_tag="ACHKW5_004096"
     CDS             complement(1319..2050)
                     /gene="pdxJ"
                     /locus_tag="ACHKW5_004096"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO7513379.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(2062..2790)
                     /gene="recO"
                     /locus_tag="ACHKW5_004097"
     CDS             complement(2062..2790)
                     /gene="recO"
                     /locus_tag="ACHKW5_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO7513380.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2802..3707)
                     /gene="era"
                     /locus_tag="ACHKW5_004098"
     CDS             complement(2802..3707)
                     /gene="era"
                     /locus_tag="ACHKW5_004098"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO7513381.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3704..4384)
                     /gene="rnc"
                     /locus_tag="ACHKW5_004099"
     CDS             complement(3704..4384)
                     /gene="rnc"
                     /locus_tag="ACHKW5_004099"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO7513382.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4657..5631)
                     /gene="lepB"
                     /locus_tag="ACHKW5_004100"
     CDS             complement(4657..5631)
                     /gene="lepB"
                     /locus_tag="ACHKW5_004100"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO7513383.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5647..7446)
                     /gene="lepA"
                     /locus_tag="ACHKW5_004101"
     CDS             complement(5647..7446)
                     /gene="lepA"
                     /locus_tag="ACHKW5_004101"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO7513384.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7644..8123)
                     /gene="rseC"
                     /locus_tag="ACHKW5_004102"
     CDS             complement(7644..8123)
                     /gene="rseC"
                     /locus_tag="ACHKW5_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO7513385.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(8120..9076)
                     /gene="rseB"
                     /locus_tag="ACHKW5_004103"
     CDS             complement(8120..9076)
                     /gene="rseB"
                     /locus_tag="ACHKW5_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO7513386.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(9076..9726)
                     /gene="rseA"
                     /locus_tag="ACHKW5_004104"
     CDS             complement(9076..9726)
                     /gene="rseA"
                     /locus_tag="ACHKW5_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO7513387.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9759..10334)
                     /gene="rpoE"
                     /locus_tag="ACHKW5_004105"
     CDS             complement(9759..10334)
                     /gene="rpoE"
                     /locus_tag="ACHKW5_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO7513388.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10331..10486)
                     /gene="rseD"
                     /locus_tag="ACHKW5_004106"
     CDS             complement(10331..10486)
                     /gene="rseD"
                     /locus_tag="ACHKW5_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO7513389.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10742..12364
                     /gene="nadB"
                     /locus_tag="ACHKW5_004107"
     CDS             10742..12364
                     /gene="nadB"
                     /locus_tag="ACHKW5_004107"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO7513390.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12349..13086)
                     /gene="trmN"
                     /locus_tag="ACHKW5_004108"
     CDS             complement(12349..13086)
                     /gene="trmN"
                     /locus_tag="ACHKW5_004108"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO7513391.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            13218..14552
                     /gene="srmB"
                     /locus_tag="ACHKW5_004109"
     CDS             13218..14552
                     /gene="srmB"
                     /locus_tag="ACHKW5_004109"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO7513392.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         3546 a         3886 c         3628 g         3556 t
ORIGIN      
        1 ctaaacgctg tattaattgt gatatgtgtg aacccgaatg cccgaatgag gcgatttcaa
       61 tgggagatca tatctacgag attaacagcg ataagtgtac cgaatgcgta gggcactacg
      121 agacaccaac ctgccagaag gtgtgcccga tccccaatac tattgtgaaa gatccggcgc
      181 atgtcgagac agaagaacag ttgtgggata aatttgtgct gatgcaccac gcggataaaa
      241 tttaatattc tactctggaa gtagagtatt aattatatta ctgggaagcc ttaacgccat
      301 tatatttatt taattgatga cattagcata atcattcact aagttaattt atatagtatc
      361 tgccaagaca cttatttata gttattaaag gcgcgtccga ttggttcacc ggacgcacct
      421 taagtacgtt tccttgtgtt ataagaacag aaggatcagc tgtaaaacag caatgatgat
      481 tttgatgacc cgtttaatca ggtatcggca atcagtcatt cgtttttcct taaacaagga
      541 atgcagccgt tcagattagc ccttacatct ccccaaaact gaacgtgcga gttattgagg
      601 gtgcatgctg cactccacac cagagctttg acgacaccac tcgtttcaat gggggaattc
      661 tgtggcatgg tgtaaagcac agcaaaatct tcaataacga agccaatttt aatgtactta
      721 cgaattggcg gtcacgttaa tctttccatc aatattgctt tcttcgtaaa ggctcgagtt
      781 tttatgctaa agattgcaag ttgcttgtaa aagataagta cactgatcca taatcgctgt
      841 tgttgagggt gcatgctgca caaaattaaa gttaaaaagt aaaacccccg ttccttacca
      901 gttcgggggt tttacttttt aaagagaacg gtattatttt aactttcaat aattaccgtg
      961 gcacaagcat agtggcgttc atctgccagc gttacatgca tatttacaac gccgagcttt
     1021 tccgccagtt ttaatgcctc accccatagc cgtagccgtg gtttgccgag ctcatcattg
     1081 aatacttcaa attgattaaa cgccagacca ttgcggatcc cggtgccaaa cgcttttgct
     1141 gcggcttctt tcacagcaaa acgcttcgcc agaaaacgca ccggctggtg gtgcgttttc
     1201 cagattgccc attcgttatc gcttaatacg cggcgtgcca ggcgctcacc ggatcgggcg
     1261 atcactgctt ctatgcgagc gatctctacg atatccgtgc ctaaacctaa tattgccatt
     1321 agccacgcgc ttccagcatc agacgcttca tttctgccac cgcatctttc agtccggtca
     1381 tcactgcacg accaataatg gcatgaccga tattcagttc atgcatctca gggatggcgg
     1441 caatagcttt cacgttgtga taggtcagac cgtgaccagc gttaactttc agaccgagac
     1501 tagcggcaaa ggtcgcggct ttggcgatac gcgccagctc ttgcgcctgt tcggcgtcag
     1561 ttttggcatc agcatagcaa ccggtgtgga tctcgataaa cggtgcgcca acctctgccg
     1621 cagctttgat ctgctcttca tcggcgtcga taaagagaga aacctggatc ccggcatctg
     1681 ccagacgttt gcaggcatcg cgcattttgt cacgctgccc tgcgacgtcc aggccgcctt
     1741 cggttgttac ttcctgacgc ttttccggta ccagacagca aaaatgtggc ttcgtctcaa
     1801 cggcgatcgc cagcatctct tcggtcaccg ccatctccag attcatgcgg gtatccagtg
     1861 tctgacgcag gatgcgcaca tcacggtcag taatgtggcg gcgatcttca cgtaaatgca
     1921 cggtaatgcc gtccgctccc gcctgctcag caataaacgc cgcctgcacc ggatccgggt
     1981 aagcagtacc gcgtgcgtta cgcagcgttg caatatggtc aatgttgacg cctaacagta
     2041 attcagccat gacaatcctc atcattcata atgtgttttc accgttcgct taggcataaa
     2101 ctgccggaac agttccctgc tctttaatgg tttaccgcca agatacggct taagcgccat
     2161 gcgggtaaag cgtttcgcgg cgcgtagtgt gtctgcgtca ggaaattccc gcgcgtttaa
     2221 cgcttttaac tgccttccgg tgaacgtttt attgtcgata acgacgcttg cgataaaccc
     2281 tttttcttcg cgataacgat acgtcatggt gtcatctacc ggctcgccgc tacccgcaca
     2341 atgggtaaaa ttgacgccat agcccagatg cccgagcagt gccagttcaa agcggcgcag
     2401 cgcgggttct ggcgtaccag tggcacctgc aagagactga atgcagtgca agtaatcgaa
     2461 aaagagttca gagaagcgcg tctcgtattc cagtacgcgg gagagaagtt cgttgatgta
     2521 cagaccgctg taaagcgtga taccgcttaa tggcagcgcc agcgagacag cttcagcact
     2581 gcgcagcgtt ttgacttcgc cacgcccgcc gaaacgtagc aagagagggg tgaaaggctg
     2641 taatgcacct ttcagagtag agcgtttaga gcgtgcgcct ttggcaacca gacgcacgcg
     2701 acccgattcc tccgtgaaga cgtccagcat caggctggtt tcgctccacg ggcgactatg
     2761 caggacaaat gcgcgctgcc agccttccat cggagtgact cttaaagatc gtcaacgtaa
     2821 ccgagactgc gcagtgcgcg ttcgtcgtcg gcccaaccgg atttcacttt tacccacagc
     2881 tcaaggtgga caggcgcttc gaacatttcc tgcatgtctt tacgcgcttc aatcccgatg
     2941 gttttgatct tggccccttt gttgccaata accatcttct tctgcccttc acgctcaacg
     3001 agaatcaaac cattgatgtc ataaccaccg cgttcgttag agacgaaacg ttcgatctcc
     3061 acggtcacgg agtatggcag ttcagcgccg aggaaacgca tcagtttttc gcggatgatt
     3121 tcagacgcca taaaacgctg tgagcgatcg gtgatgtaat cttccgggaa gtgatgggtc
     3181 gcttcaggga ggtgcttacg cacgattgct gcgatagtgt cgacattcag cccggtttca
     3241 gcagagatcg gcacgatatc gaggaagttc atctgacttg ccaggaactg taagtgcggc
     3301 agcagatcgg ctttttcctg cacgttatcc actttgttca ccgcgaggat caccggcgct
     3361 ttgccatcgc gcagtttgtt gagcaccatt tcgtcgtccg gcgtccagcg ggtgccttca
     3421 acgacaaaaa tcaccagctc aacgtcgcca atagagctgc tcgccgcttt gttcatcagg
     3481 cggttaatag cgcgtttttc ttccatatgc aggccagggg tatcaacgta gatcgcctga
     3541 tacgcgcctt cagtatggat ccccacaatg cggtgacgag ttgtttgcgc tttgcgggag
     3601 gtgatggaga tcttctgccc cagcagtttg ttcaacaatg tggatttgcc aacgttcgga
     3661 cgtccgacga tggcaataaa tccgcagtaa cttttatcga tgctcattcc agctccagtt
     3721 ttttcaacgc ctgttcggcg gcagcctgct cagccttacg acggcttgaa cctgtgccaa
     3781 ccaccggttc actcaggccg ctgacctggc agtggatagt aaattcctga tcgtgcgctt
     3841 cgccacgtac ctggactacc agataagtcg gcagcggcag atggcgaccc tgcaaatatt
     3901 cttgcaagcg cgttttcgga tctttttgtt tatcgcctgg gctaatttcg tccaaacgag
     3961 tttgatacca gttgaggatt aatttctcga cggtttgaat atcactgtcg aggaatacgc
     4021 caccaattaa tgcttcgacg gtgtcggcga gaattgactc acgacgaaat ccaccgcttt
     4081 taagttcacc tggccctaaa cgtaagcact cgcctaactc aaattcgcgc gccagttccg
     4141 ccagcgtatt gccacggacc agcgtggcgc gcatccggct catatcgcct tcatccacac
     4201 gagggaaacg gtgataaagc gcattggcga taacgtagct cagaatagag tcgcctaaaa
     4261 attctaaacg ctcgttatgt ttactgctgg cactacgatg agttaatgcc tgctgcaaca
     4321 gttcctgatg attaaaagtg tagcccagct tccgttgaag ccgattaatt acgatggggt
     4381 tcatgcgata ccaataaata aatgcgtcaa caattcagca cacgaaacag acctgatata
     4441 catggctctg ctaactgctt cgctgcagtt tcagtttacc ggtatatggg gaccaacgct
     4501 gtttcgtgtg ccgtggcaac ctggaggtgc caaccttaaa cttcggggga atattctata
     4561 cacaacgacg ggggatgtcg ttagccacgg gagatttatc tcataaataa ttcacgttgt
     4621 cgccataacg gcgacaacgt gaacgaagat gagctattaa tggatgccgc caatgcgact
     4681 taagcgcaca ccagtcggcc attcgccttc ttgcttatcg aagctcatcc agatagccgt
     4741 tgcccgaccg accagattcg cttcaggcac aaacccccaa taacggctgt ccgcgctgtt
     4801 gtcgcggttg tcgcccatca tgaagtattg tcccggagga acaatccagg ttgccagttg
     4861 ttgccctggc tgctggtaat acatccccac ctgatcctgc gcaatcggca ctgtcagaat
     4921 tcggtgcgtc acatcaccca gtgtctcttt acgctcggaa agacgaattc cattttcttt
     4981 ggtttcgttt ttcggcactt caaagaatcc gctggtcgct tccccaccat tacggcgtga
     5041 gaaggtctga acgaaatcgc tcggttccac gtttgagtag gtgaccggca gcgcgttttc
     5101 acacgcctgg ccggaactgc atcccggttg aatcgtcagc tctttcgaga ccggatcata
     5161 agtaacttta tcgcccggta aacccaccgc gcgcttgatg taatcaagct ttggatcttc
     5221 cggatattta aagaccacga tatctccgcg tttcggatga ccggtttcga tcagcgtttt
     5281 ctggtagata ggatctttaa tgccataagc aaacttttct accagaataa aatcaccgat
     5341 taacagagtc ggcatcatcg aacctgacgg gatctggaac ggttcataaa taaacgaacg
     5401 cacaatcaat acgatagcca gtaccggaaa aacagaagca ccggtttcca gccagccagg
     5461 cttcggcgca acctttttca acgttgcttt atccagtgag tcaccggcag ccgcctgcgc
     5521 cgctgcctga cgttcccgcc gtttaggtgc gaaaaagaat ttatccacgc accataaaat
     5581 gcccgtcacc agtgtggcaa tcaccagaat cagggcaaac atattcgcca tgccaactcc
     5641 taagggttat ttgttgtctt tgccgacgtg cagaatggcg aggaacgctt cctgcggcag
     5701 ctcgacgtta ccgatctgct tcatgcgttt cttaccttct ttctgcttct gcagcagctt
     5761 tttcttacgg ctgatatcgc cgccataaca tttagccagt acgtttttac gcagctgttt
     5821 cacggtggat cgcgcaataa tgtgcgtacc aatcgctgcc tgaatggcga tatcaaactg
     5881 ctggcgtggg atcagatctt tcatcttctc caccaactcg cgaccgcggt tttgcgaatt
     5941 atcacggtgg gtgatcagcg ccaacgcatc aacacgttca ccgttgatta atacgtctac
     6001 acgtaccatg tcggacgcct ggaagcgttt gaagttgtaa tccagagacg cataaccacg
     6061 cgaggtagat ttcaggcgat cgaagaagtc gagcaccact tccgccatcg ggatctcgta
     6121 cgtcagcgcc acctgattac cgtggtaaac catattggtc tgcacgccgc gtttttctac
     6181 gcacagcgta ataacgttgc cgagatatgc ctgcggcagc agcatgtgac attcggcaat
     6241 cggctcgcgc agttcgtaga tgttatttac cgctggcagc ttggatggac tgtcgacgta
     6301 aataacttct cttgacgtgg tttcaacttc atacactacg gtcggcgcag tggtgatcag
     6361 atccagatcg tattcacgtt ccagacgttc ctggatgatc tccatgtgca gcaggccgag
     6421 gaagccgcag cggaaaccaa agcccagcgc gctggagctt tccggctcat agaacagtga
     6481 agcatcgttc aggctgagtt tacccagcgc gtcacggaag gcttcatagt cgtcggaact
     6541 taccgggaac agaccggcgt atacctgcgg tttgactttc ttaaagccag gcagcgcctt
     6601 ttctgccgga ttacgcgcca gcgttaaggt atcgccgact ggagcgccgt ggatatcttt
     6661 aatcgcacat accagccagc ctacttcgcc acatttcagt tcagtgcggt caacctgttt
     6721 cggcgtgaag atgcccagac ggtcggcgtt ataggtctgc ccggtactca tgactttcac
     6781 tttgtcgccc ttacgcaggg tgccgttttt aatacggata agtgaaacaa cgcccaggta
     6841 gttgtcgaac catgagtcga taattagtgc ctgcaacggg ccttccggat cgccttccgg
     6901 cggcggaatg tcgcgcacca gacgttcgag aacgtcctgc acgccaacgc cggttttcgc
     6961 tgaacagcgc accgcgtcag tggcgtcgat gccgacgata tcttcaattt cttccgccac
     7021 gcgttcagga tcggctgccg gcaggtcaat cttgttcagt actggcacca cttcgagatc
     7081 catttccatg gcggtgtagc agtttgccag ggtttgcgct tccacgccct gcccggcgtc
     7141 gaccaccagc aatgcacctt cacaggcagc cagcgaacgg gaaacttcat aggagaagtc
     7201 tacgtggccc ggggtgtcga taaagttaag ctgataggtt tcgccgtcag acgctttgta
     7261 gtccagcgtc acgctttgcg ctttgatggt aatgccacgc tcacgctcaa gatccatgga
     7321 atcgagaacc tgcgcctcca tttcacggtc agacaggcca ccgcagatct ggataatacg
     7381 gtcagacagc gtcgatttac cgtggtcaat gtgagctatg atcgaaaagt tacgtatatt
     7441 cttcataaag tatgattatt gtgccttaat gcccggttaa ccaggctttt agaagtcgct
     7501 gttctgagct taacgtctgt attaatagaa acgccgcatt ctacactaca acattgaggc
     7561 gaggaaatgt tcataccgta tggattgtgg tatctggaaa cgtcctcgca tttgttatgc
     7621 aaaatgcaac aaagccagtg aaatcactgg ctcgcgtctt ccgaagatgt ttcaaatcgc
     7681 acaaggccag gcggcaatgc cacgcttaag atgatcggtt gccattctgc ccgggcagca
     7741 aacttacgcg agtagccgcg cgcaatcagg aacccgccaa taccaccgag gatcgcacca
     7801 cataatgctg cgacgtcaga agcaaagagt agctgaaata gcgaagcgat aaggaataat
     7861 cccaccagcg gcgacatata aaccagtaat gcggagctaa gcaggctgcc ttcggcgatc
     7921 cccaattcca ctttttgccc cggcactaac ggttcatcac agggtacgac aatggtatgc
     7981 gtggtttgcg ggccaagttt attcaacacg cggctaccgc aaccggcgcg tgaagcgcag
     8041 ctgctgcatg aagctttaac atcacaactg accagcgcct gcccgttttg ccaggagacg
     8101 acggtagccc actctttgat cattgcgctg ccccgaactt aatattctcg gcaatgcgtt
     8161 tcgccgtttg cggcggcagt tcaccgacaa tggtgatttc ggcgttatca cgtacgcttg
     8221 tactgacggt tctgcgtccg gtgcgcaaca tctgatcggt gctcgatggc gtagcgcggt
     8281 taacgtttac cgagaagctg aataatccgt cggaatagag acgtgattcg ataggcatgt
     8341 tgtccatcgt cggtagcgga cgtcgactac tggaaacttc gctaaaaccc tgtggcaacc
     8401 aggttggcgt ccagctgaat ttagcttttt cacctacagg aacagaaagc aacggcggca
     8461 aatttgcctt cgccagcgtc tgcatactgc tactgatatc ctgattgacg ttaaaagcaa
     8521 tcacgcgaaa ttgttccagc gtttcaccat cgcgatcaag gagatcaacc ctcatcggta
     8581 atttcgattc gatgtccatc cacacgatgt agctgtagcg tgtaccatct cgggcaacca
     8641 cgcgaatgac ttcgcaaaga cgatcagcaa tacgcgtgcg cccgacggag ataaagtcgt
     8701 agtaaggaga aaggcgtttg aaatcggtat agatgagcga tggcagagaa tcaacaatgt
     8761 aatcgccatt aagcgtgaac ggttcaagtc ccggttcaaa atagctgatt tcattgccgc
     8821 gctgtaccac ttcccggcgc gggccatcca tttgcaacaa ttgtgcaaga ggacggttat
     8881 cgaggcgtgc atgtcgataa cgcagagact caacaccctg tttattgatg ctgatgaatg
     8941 acagctcgta attcagtgac tgactggcca ggttcatctg ctgtaataac gccccggacg
     9001 cgggagtggc cgaggcgtta gcagagaata acaggctacc tgtcactaat gacatggcaa
     9061 accaaagttg cttcattact gcgattgcgt tcctaaagtt tgaattcctg gcacctgtac
     9121 agcggcttgc tgggtttgcg cctgctcaaa ctgaagctgt tcagagtgga gtcggcgttg
     9181 cagttcgtaa tcctgcaaca ttgcattaat gcgacgacgc tgctcctgta cctgctgctg
     9241 ttgaccattg tttgcggtcg cttcagaagg tactcccagg cttaccgggc tggctttacc
     9301 catcatcggc agtgtattaa ataccggcgt ttcgggctgc tgggacgttt cagattgtcc
     9361 attatagtgc tggacgccaa cgataactgc aagcgatacg catgctgcta cgcccatttg
     9421 ggtaagctgt gccgcccacg gacgtacttt ctgccagaat ggcattttct gccattgatg
     9481 cggcgcaggc tgggcttccg ggatcaatgt cgccggttga cgtactggct cttcttcaat
     9541 ggcggccatc acgcgtgaag agatatcgaa atggagcacc tcgggagtat caccccgcat
     9601 tgagtcacgg attaagtgat agctttccca ggttttctgc atttctgggt tatgagccag
     9661 ttcgttaagc agctcactat ccagcgtttc gccatccatt aaagcggaaa gttgttcttt
     9721 ctgcatgcct aataccctta tccagtatcc cgctatcgtc aacgcctgat aagcggttga
     9781 actttgttat caatagcttc cctcgctcgg aagatacgtg aacgcaccgt acctaccgga
     9841 caatccatga tagcggctat ctcttcatag ctcaggccat ccagctcccg caaggttatt
     9901 gccatgcgca aatcttccgg gagggactca atagttcgga aaactatctg tctcagttct
     9961 tctgacaaca ttaagttctc agggttcgaa atttctttca acgcgccgcc actttcgaag
    10021 ttttcagctt caatggcatc cacatcactg gaaggtggac gacgcccctg agcaaccagg
    10081 taatttttcg ctgtatttac agcaatccga tacagccatg tataaaaagc gctatctccc
    10141 cggaacgaat ccagcgcacg ataggcttta ataaaagctt cttgtaccac atcgggaaca
    10201 tcacccgacg gcacatagcg ggaaaccaga ctcgccactt tatgctgata gcgcactacc
    10261 agtaggttaa aggctttctg atctcccttc tggacccgtt caaccaggac ctggtccgtt
    10321 aactgctcgc tcatccgagg taaagtctcc ccaaaccaaa tttccacgcg ctatcgaaac
    10381 gccactccat tagctgcaat ttgagcaagc aaagggttag agtgtctcgt ttttgtaaag
    10441 ttccgtaacg catctgtttt tgtttgtcat gctgtagacg gatcattatc tatcattata
    10501 agtctacaga atctgaacat cgcattatct gtgtagaaat gcccatttaa ctgcctgaag
    10561 agtaacccaa cggccttttt atttcaccac ctaatcctcc accagccagt aacttctctt
    10621 tttctcgccg ccctgcgtca gcgtgtttag ccactgtaac aaatattaaa atagcaggtg
    10681 tttattcgca caacatgatg ctatgctgac caaacggtgt ttagtaaatt aaacaaagaa
    10741 aatgaatact ctcccagaac attcatgtga cgtgttgatt atcggtagcg gcgcagccgg
    10801 actttcactg gcgctacgcc tggctgacca gcatcaggtc atcgttctaa gtaaaggccc
    10861 ggtaacggaa ggttcaacat tttatgccca gggcggtatt gccgccgtgt ttgatgaaac
    10921 tgacagcatt gactcgcatg tggaagacac attgattgcc ggggctggta tttgcgatcg
    10981 ccatgcagtt gaatttgtcg ccagcaatgc acgatcctgt gtgcaatggt taattgacca
    11041 aggggttttg tttgataccc acgttcaacc gaatggcgaa gaaagctacc atctgacccg
    11101 tgaaggtgga catagtcacc gtcgtattct tcatgccgcc gacgccaccg gtagagaagt
    11161 acaatccacg ctggtgagca aggcgcagaa ccatccgaat attcgtgtgc tggagcgcag
    11221 caacgcagtt gatctgattg tttctgacaa aattggcctc ccgggcacgc gacgggttgt
    11281 tggcgcgtgg gtatggaacc gaaataaaga aacggtggaa acctgccacg caaaagcagt
    11341 ggtactggca accggcggtg cgtcgaaggt ttatcagtac accaccaatc cggatatttc
    11401 ttctggcgat ggcattgcta tggcgtggcg cgcaggctgt cgggttgcca atctcgaatt
    11461 taatcagttc caccctaccg cactgtatca cccacaggca cgcaatttcc tgttaacgga
    11521 agcactgcgc ggcgaaggcg cttatctcaa gcgcccggat ggcacgcgtt ttatgcccga
    11581 ttttgatgag cgcggcgaac tggccccgcg cgatattgtc gcccgcgcca ttgaccatga
    11641 aatgaaacgc ctcggcgcag attgtatgtt cctcgacatc agccataagc ccgccgattt
    11701 tattcgccag catttcccga tgatttatga aaaattgctc gggctgggga ttgatctcac
    11761 acaagaaccg gtaccgattg tgcctgctgc acattatacc tgcggtggtg taatggttga
    11821 tgatcatggg cgtacggacg tcgagggctt gtatgccatt ggcgaggtga gttataccgg
    11881 cttacacggc gctaaccgca tggcctcgaa ttcattgctg gagtgtctgg tctatggctg
    11941 gtcggcggca gaagatatca ccagacgtat gcctgatgcc cacggcgtca gtacgttacc
    12001 gccgtgggat gaaagccgcg ttgagaaccc tgacgaacgg gtagtaattc agcataactg
    12061 gcacgagcta cggctgttta tgtgggatta cgttggcatt gtgcgcacaa cgaagcgcct
    12121 ggaacgcgcc ctgcggcgga taaccatgct ccaacaagaa atagacgaat attacgccca
    12181 tttccgcgtc tcaaataatt tgctggagct gcgtaatctg gtacaggttg ccgagttgat
    12241 tgttcgctgc gcgatgatgc gcaaagaaag ccgggggctg cattttacgc tggattaccc
    12301 ggaactgctc acgcattccg gtccatcgat cctttccccc ggcaatcatt acataaacag
    12361 ataaaaagcc tgggtcagcg ccgtatacgc ttcggaatag ttctggtctg gcccacgaat
    12421 gactaagcga tcgctaaagc attctcccgc ctgcggggag aatgccagca gcacccgatg
    12481 cggcagtcgc gcttcgtttt ccgccacatc cgtccgcaaa cgtaaatgcc agcccatgct
    12541 taatgccagt tcagtaaaac cattaccaat ctgctctggc agcaccacgc agaaaaatcc
    12601 ctcttcggta atgcactccg ccgcacaggt cagcagcgat gggtgatcaa gcgtagtggt
    12661 atagcgagcc tgttcccgtt gaggtgtcgc gcactctact ccctgctgat agtaaggtgg
    12721 gttactgatg attaaatcga agcgtactgt ctgctgtgtg atccactgct gaatatccgc
    12781 cgtatggacg ttaatccgct ctgcccacgg ggactggttg atattttcct gcgcctgcgc
    12841 ggcagcttca ctttccagct caacggcatc aattatcacg ctgtcactgg ttcgctgcgc
    12901 cagcatcagt gccagcaacc cgctacccgc gccgatatca aggcaacgtt tcaccccagc
    12961 caccggtgcc catgcgccca gcaaaatgcc atccgttccc actttcatcg cacagcgatc
    13021 gtgagcaaca aaaaactgtt taaaagtaaa tccattacga cgaagcacgg atgtagactg
    13081 tgacatgaaa ataaaacctt gcaggaaaaa cggcgatagc accgggtgag aacaataccc
    13141 gagaagcgat atccatacaa acagatgaag attgcagccg taacgtctat aatcagcgcc
    13201 ccacacagag gtagaacatg actgtaacga ctttttccga acttgaactc gacgaaagcc
    13261 tgctggaagc cctccaggat aaaggtttca ctcgcccgac cgccattcag gctgccgcca
    13321 ttccgcctgc gctcgatggc cgtgatgtac tcggttctgc gccgacaggc accggtaaaa
    13381 cggcggcgta tctgctgcca gcgttgcagc acctgctcga tttcccgcgt aagaaatccg
    13441 gtccgccgcg tattttgatc ctcaccccaa ctcgcgagct ggcgatgcag gtggccgatc
    13501 atgcccgcga actggcgaaa catacgcatc tggatatcgc caccatcacc ggcggcgtag
    13561 cctatatgaa ccacgcggaa gtgttcagcg aaaatcagga catcgtggtc gccacgaccg
    13621 gacgtctgct gcaatacata aaagaagaga acttcgattg ccgcgcggtt gaaacgctga
    13681 tcctcgacga agcagaccgt atgctggata tgggcttcgc tcaggatatc gaacatattg
    13741 ctggcgaaac gcgctggcgt aaacagaccc tgctcttttc ggcaacgctg gaaggcgatg
    13801 cgattcagga ctttgccgag cgtctgctgg aagatccggt ggaggtttct gccaatccgt
    13861 ccacccgtga gcgcaaaaaa attcatcagt ggtattaccg cgccgatgac ctggaacata
    13921 aaaccgcatt gctggtgcat ctgttaaaac agccggaagc gacccgctca attgtgtttg
    13981 tgcgtaagcg tgaacgtgtg catgagctgg caaactggct gcgcgaagcg ggcatcaaca
    14041 actgctatct cgaaggtgag atggtacaag gcaagcgtaa cgaagcgatc aagcgtttga
    14101 ccgaaggtcg cgtaaacgta ctggttgcaa ccgatgttgc cgcgcgcggt atcgacattc
    14161 ctgacgtcag ccacgtcttt aacttcgata tgccgcgcag tggcgatact tatttgcacc
    14221 gtatcggacg taccgcgcgc gccggtcgta aaggcaccgc aatttcgctg gtggaagccc
    14281 atgaccattt gctgctgggc aaggttggcc gttacattga agagccgatt aaagctcgcg
    14341 ttattgatga gttacgcccg aaaacgcgtg cgccaagcga gaagcagacc ggcaagccat
    14401 cgaagaaagt actggcaaaa cgcgccgaga agaaaaaagc taaagagaaa gagaaaccgc
    14461 gtgtgaaaaa acgccatcgc gacaccaaaa atattggtaa gcgccgtaaa ccaagcggaa
    14521 cgggcgtgcc accgcaaacg acagaagagt aatctcaatg ccgggtttaa gcctggtatt
    14581 aaaagtgctg gcaaacgcaa aactgcctga tgcgct
//
LOCUS       DAZFYC010000123        14605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFYC010000123 DAZFYC010000000
VERSION     DAZFYC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.123"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHKW5_004110"
     CDS             <1..112
                     /locus_tag="ACHKW5_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7513393.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            171..374
                     /locus_tag="ACHKW5_004111"
     CDS             171..374
                     /locus_tag="ACHKW5_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7513394.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            374..1424
                     /locus_tag="ACHKW5_004112"
                     /pseudo
     CDS             374..1424
                     /locus_tag="ACHKW5_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1452..1541)
                     /locus_tag="ACHKW5_004113"
     tRNA            complement(1452..1541)
                     /locus_tag="ACHKW5_004113"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Ser,seq:cga)
     gene            1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHKW5_004114"
     CDS             1635..2432
                     /gene="mtfA"
                     /locus_tag="ACHKW5_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO7513395.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            2533..2608
                     /locus_tag="ACHKW5_004115"
     tRNA            2533..2608
                     /locus_tag="ACHKW5_004115"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2566..2568,aa:Asn,seq:gtt)
     gene            2922..9999
                     /gene="yeeJ"
                     /locus_tag="ACHKW5_004116"
                     /pseudo
     CDS             2922..9999
                     /gene="yeeJ"
                     /locus_tag="ACHKW5_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(10261..11313)
                     /locus_tag="ACHKW5_004117"
     CDS             complement(10261..11313)
                     /locus_tag="ACHKW5_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO7513396.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQKYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            11628..12944
                     /gene="shiA"
                     /locus_tag="ACHKW5_004118"
     CDS             11628..12944
                     /gene="shiA"
                     /locus_tag="ACHKW5_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO7513397.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            13046..14500
                     /gene="amn"
                     /locus_tag="ACHKW5_004119"
     CDS             13046..14500
                     /gene="amn"
                     /locus_tag="ACHKW5_004119"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO7513398.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         3989 a         3475 c         3459 g         3682 t
ORIGIN      
        1 ggctattcca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaagcaaa
       61 atacgtttca gttatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 cctaattgcc gctaaccgta tatagttagc ggcggttatg agatatagct atgagtagct
      181 tatttttaac cgaagatgaa ttgctaatat taacgggctg caaatatgca agccaccagc
      241 gaaaatggtt aacggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg
      301 tcagccggga tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt
      361 ttggtgcaat ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaac tacctccaaa
      421 tgtataccca aataaatata gttatgtatg gaaaccaaca tccagagaat ctgtcacact
      481 aaccgccatc aaggatggtt tagctgcttt atggaaaagg tatgaggaaa ctgtaaataa
      541 tcgcgatcgc gcaatgacat tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta
      601 cagtgacctc agtccaagaa cacaaaaaga ttatctacaa catcaaaaaa agttgcttgc
      661 cgtattcggt aaggtgccgg cagattccat aaaaccagaa cacatccgtc gatacatgga
      721 caagagaggg gagcagagta aaacgcaagc caaccatgaa aaaagcagta tgtcccgcgt
      781 ttacagttgg gggtatgagc gagggtacgt gaaaggtaac ccatgtgcag gtgtaagtaa
      841 attcaaggcc aaaaaccgcg aacgatatgt aaccgacaaa gaataccagg cagtattaag
      901 cgttgcacct cttcctgttt ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt
      961 ttccgatgtg ttatcgctga aatgggagca gattggaaac gacggaatct ttatccagca
     1021 agggaaaaca ggaaaaaaac agataaaagc atggagtcca cgattacagg cggcgatcga
     1081 aaaagcaaaa cagttaccaa catccgccta tgtaatcagt aatcaatacg gcaaccgata
     1141 tatgtacaaa ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc
     1201 aggtatttta accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga
     1261 aggaagcagt cgggataagc aacttttctc tggtcacaaa accgaggggc aagtgctaat
     1321 ctatgacagg aaggttaaag tttcaccgac acttgatgtc ccgttacctg aaaatattcc
     1381 aagaaaatat tccaagtaat tccaagtgtg atttttgtca ctgacttaat gatgtataag
     1441 tgattgaatt ttggcggaga gagggggatt tgaacccccg gtggagttgc ccccactccg
     1501 gttttcgaga ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga
     1561 tgccaggaaa tttggcattt taacagtccc tgtccgtgca attttgttca agtgacgagt
     1621 ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg
     1681 cccttccctg gcaggaagca ctatcgatcc cccttttaac gtgtctgacg gaacaggaac
     1741 aaagcaaatt agtcactctt gccgaacgtt ttttacagca aaagcggctt gttcctttac
     1801 agggctttga actggattca ttaagaagct gccggatagc acttctattt tgcctacccg
     1861 ttctggagtt aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc
     1921 catttgtggt cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg
     1981 ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag
     2041 attcttttga tgcttctggt tttaacctga ttattcatga agtcgctcat aagctggaca
     2101 cccgtaacgg cgatcgcgcc agcggagttc cctttattcc gttgcgtgag gttgctggct
     2161 gggaacacga tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttggttggcg
     2221 agaatgcggc gagcattgat gcttatgctg ccagtgatcc tgctgaatgt tttgccgtac
     2281 tttctgaata tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc
     2341 aacgtttctg ccaattttat caacaagatc ctttgcagag actgcatcgc actaatgata
     2401 cagactcgtt ttcggcgacg aatgttcatt aattaacaac tttgcagatt aattaaccaa
     2461 ttgaaatgac ttatgaaatt tagtgttgac agacaaggta ccgctaagta atatgcgccc
     2521 cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
     2581 actggttcga gtccagtcag aggagccaaa ttctaaaaat tcgctttttt agcgcaatgt
     2641 cactgacctt agttgaacat tgttttttaa cggatagcgg gtttttaaca tcttaagcgc
     2701 cctcgacctt tatggttgag ggcgttttgc tatgaacgcc atcaccattt tcccctcgat
     2761 tataaaactt gagttattca gtagtctccc ctcttgcaac tcacacccaa aactgcctaa
     2821 cgaaaagtta ttaattttca atcatattgc tatcaggatt tacatttttt cgctgtgcta
     2881 gaaagagcgc atttatgtta gctcgttcag ggaaggtaag catggctacg aagaagagaa
     2941 gtggagaaga aataaatgac cgacaaatct tatgcgggat gggaattaaa ctacgccgct
     3001 taactgcggg tatctgcctg ataactcaac ttgcgttccc tatggctgcg gcagcacaag
     3061 gtgtggtaaa cgccgcaacc caacaaccag ttcctgcaca actcgccatt gcaaatgcca
     3121 atacggtgcc ctacaccctt ggagcgctgg aaatcggccc aaagcgttgc cgaacgtttc
     3181 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
     3241 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaataattta
     3301 accccgccac cgggtaatag cagcggcaac cttgagcaac agatagccag tacttcacag
     3361 caaatcgggt ctctgctcgc cgaggatatg aacagcgagc aagcggcaaa tatggcgcgt
     3421 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
     3481 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
     3541 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
     3601 actgacgagc gtacacagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
     3661 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
     3721 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
     3781 accaactggc gcagcgcacc tgaactggac aacgattatg aagcacgccc ggccaatggc
     3841 tgggatgtac gcgcagaagg ctggctaccc gcctggccgt accttggcgg taaactggtc
     3901 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
     3961 cctcatgcca taaccgctgg acttaactat acccccttcc cgctaatgac cttcagcgcg
     4021 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
     4081 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
     4141 cttgcaggca gccgttatga tctggtggat cgcaacaaca acatcgttct ggaatatcgc
     4201 aaaaaagaac tggtccgcct gaccctgaca gaccccgtga cagggaagtc aggagaagtg
     4261 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
     4321 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
     4381 ctgccgggtt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
     4441 accgctgaag atgtcaaagg caatttgtcg aatcgtgaac aaagcatggt ggtcgttcag
     4501 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
     4561 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
     4621 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgaatgg
     4681 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
     4741 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
     4801 atcatttcta tttcgtcatc ccgaactcac tcgtcaatta aaattgataa agaccgttat
     4861 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
     4921 aaggagcaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa acctggtgtc
     4981 actacagact ggaaagaaac cgcagatggc gtctataagg caacctatac cgcctatacc
     5041 agaggcagtg ggcttactgc gaagctgtta atgcaaaact ggaatgaaga tttgcatacc
     5101 gctggattta tcatcgacgc caacccgcag tcagcaaaaa ttgcgacatt atctgccagc
     5161 aataatggtg tgctcgccaa tgagaatgca gcaaacaccg tctcggtcaa tgtcgctgat
     5221 gaaggaagca acccaatcaa tgatcatacc gtcacgtttg cggtattaag cgggtcggca
     5281 acttgcttta acaatcaaaa caccgcaaaa acggatgtta atggtctggc gacttttgat
     5341 ctgaaaagta gtaagcagga agacaacacg gttgaagtca cccttgaaaa tggcgtgaaa
     5401 caaacgttaa ttgtcagttt tgtcggcgac tcgagtaccg cgcaggttga tctgcagaag
     5461 tcgaaaaatg aagtggtcgc tgacggcaat gatagcgcca caatgaccgc gacagtccgg
     5521 gatgcaaaag gcaacctgct caatgacgtc aaggtcactt tcaatgttaa ttcagcagca
     5581 gcgaaactga gccaaaccga agtgaatagc cacgacggga tcgccacagc tacgctgacc
     5641 agtttgaaaa atggtgatta tagggttacg gcctctgtga gctctggttc tcaggctaat
     5701 caacaggtga tttttatcgg tgatcaaagt actgctgccc tgaccctcag tgtgccttca
     5761 ggtgatatca ccgtcaccaa cacagctccg ctacatatga ctgcaacctt gcaggataaa
     5821 aatggcaacc cattaaaaga taaagaaatc accttctctg tgccaaacga cgtcgcaagt
     5881 cggttctcga ttagcaacag cggaaaaggc atgacggata gcaacgggac tgcaatcgcc
     5941 tccctgaccg gcacgttagc gggcacgcat atgatcacgg ctcgtctggc taacagcaat
     6001 gtcagcgata cacagccaat gacgtttgtg gcggataaag acagagcggt tgtcgttctg
     6061 caaacatcga aagcggaaat cattgggaat ggcgtggatg agacaactct gacagcaaca
     6121 gtgaaagatc cgtcgaatca tccggtggcg gggataacgg tcaacttcac catgccacag
     6181 gacgttgcgg caaactttac ccttgaaaat aacggtattg ccatcactca ggccaatggg
     6241 gaagcgcatg tcacgctgaa aggtaaaaaa gcgggcacgc atacggttac cgcaacgctg
     6301 ggtaataaca ataccagtga ttcgcagccg gtaacatttg tggcggacaa agcctcggct
     6361 caggttgtcc tgcagatatc aaaagatgag atcacaggta atggcgtcga tagcgcaacg
     6421 ctaactgcaa cggttaaaga tcagttcgac aatgaggtga ataatcttcc ggtaacattc
     6481 agctcagcct cttcaggact caccctgacc ccgggagtga gtaataccaa tgagtctggc
     6541 atcgcgcagg ccactatcgc aggcgttgcc tttggtgagc agacggtcac ggcatcactg
     6601 gctaataatg gtgccagcga caacaaaact gtgcatttta ttggcgacac agcggcggca
     6661 aaaattatcg agttgacgcc tgtcccagac agcataatcg ccggtacccc gcagaacagc
     6721 accggcagcg tcatcaccgc cacagtcgtt gataataatg gctttccggt gaaaggtgtg
     6781 actgtgaact tcaccagccg aacaaactct gccgaaatga cgaatggcgg ccaagccgta
     6841 acgaacgaac agggtaaggc aaccgtcact tataccaata cccgctcctc gatagaatca
     6901 ggagcgagac cggataccgt tgaggccagt ctggaaaatg gtaactccac gcttagcaca
     6961 tcaattaatg tcaacgcgga tgcgtctaca gcacatctca ccttgctaca tgcacttttt
     7021 gatacagtct ccgcaggcga gacaaccagt ctgtatattg aggtgaagga taattacggc
     7081 aatggtgtac cccagcatca ggtaaccctc agcgtttcac caagtgaagg cgtgaccctc
     7141 agtaataacg gtatatatac taccaactac tacggctatt tttacgcaag ctttaccgct
     7201 acaaaagccg gggtttatca agtgacggca accctcgaca atggcgattc gatgcaacaa
     7261 acagtgacct atgtgccgaa cgtcgcgaat gctgaaatca cgctggcagc ctcgaaggat
     7321 ccggtgattg ccgacaataa cgatctcaca acactaacag caacagtcgc tgatacagag
     7381 ggcaatgcga tagccaacac tgaggtaaca tttactctgc cggaagatgt gagggcgaac
     7441 ttcacgctga gcgatggcgg taaagcgatt actgatactg aaggcaaagc gaaagtcacg
     7501 ctgaaaggta taaaagcagg cgctcatact gttacagcat cgatggctgg cagtaagagt
     7561 ggacaactgg tggtgaactt tactgcggat acgctcactg cgcaggttaa tcttaacgtt
     7621 accgaggaca atttcatcgc caataacatt gggatgacca agctgcaggc aacagtgact
     7681 gatggaaacg gcaacccatt cgccaatgag gcggtgacat tcacgctacc ggcagatgtg
     7741 agcgcaagct tcactctcgg acaaggcggt tccgccatta ctgatatcaa cggcaaggct
     7801 gaagttacac tgagcggtac caaatccggc acctatcccg tgacagttag cgtgatcaat
     7861 tatggtgtca gtgatacgaa acaggtgact ttgattgccg atgctggtac cgcaacacta
     7921 gcctccttaa cctctgtata ctcattcgtc gttagcacga ccgagggcgc gaccatgact
     7981 gcaagcgtca ctgacgctaa cggcaacccg gtagaaggca taaaagttaa tttccgcgga
     8041 acctccgtca cgctaagcag caccagcgtt gaaacggatg atcagggttt cgctgaaatt
     8101 cttgtgacaa gcaccgaagt cggactgaaa acagtttcag cctctctggc agataaacct
     8161 actgaagtca tatcgcgatt actgaatgca aaagcagata ttaattctgc aacgattacc
     8221 agtctggaga tacctgaagg tcagctaatg gtcgcacaag acgtagcagt taaagctcac
     8281 gtcaacgacc agtttggcaa cccgattctt aatgaatctg taacattcag tgcagagcca
     8341 ccagagcaca tgaccatcag ccaaaatatt gtctctactg atacgcatgg tatagccgag
     8401 gtctccatga cgcccgaaag aaacggtttg tatatggtga aagcatccct ggcgaatgga
     8461 gcctcacttg agaaacaact ggaggctatt gatgaaaaac tgacactcac ggcgtccagt
     8521 ccgcttatcg gtgtctatgc ccctacaggc actactctga cggcaacgct aacctctgca
     8581 aatggcactc cagtggaggg tcaggtcatc aactttagcg taacgccaga aggggcgacg
     8641 ttaagtggcg gaaaagtgag aactaactct tcaggtcagg ctccggtcgt tctgaccagc
     8701 aataaagtcg gtacatatac ggtgactgca tcgttccata acggcgtaac aatacagaca
     8761 cagacaaccg tgaaagtcac tggcaactca agcaccgcac atgttgctag ctttatcgct
     8821 gatccatcga ctatcgccgc caccaacagt gatttaagta ccttaaaggc aacggttgag
     8881 gatggcagtg gtaacctgat cgaaggtctc actgtgtact tcgccttaaa aagcggctct
     8941 gccacattaa cgtcattaac agcggtgacc gatcaaaacg gaatcgcgac aacaagcgtg
     9001 aaaggagcga tgacaggtag cgtcacggta agcgcagtca cgaccgctgg tggaatgcaa
     9061 acagtagata taacgctggt ggcaggcccg gcagacgcct cgcagtccgt ccttaagaac
     9121 aatcggtcct cactgaaagg ggactttacc gatagtgctg agctacatct tgttttgcac
     9181 gatatatcag gcaatccgat caaagtttct gaagggatgg aatttgtgca atcaggtact
     9241 aacgtgccct atatgaaaat tagcgcaatt gattacagtc aaaatatcaa cggtgattac
     9301 aaagccacca ttacaggcgg cggagagggt atcgcaacgc tgatccctgt attgaatggt
     9361 gttcatcaag ctggtctgag taccacaata caattcactc gcgcagaaga caaaataatg
     9421 agcggtacag tatcagtcaa tggtactgac ctaccgacaa ctacattccc ttcgcagggg
     9481 ttcaccgggg cgtattatca gttgaataat gacaactttg ccccaggaaa aacggcggct
     9541 gattatgagt tttcaagctc tgcctcctgg gtcgatgttg atgctaccgg taaagtgaca
     9601 tttaaaaatg tcggcagcaa ttgggaaagg attacggcga cgccaaaatc aggaggccct
     9661 agctatgtat acgaaatccg tgtgaagagt tggtgggtga actccggcga tgctttcatg
     9721 atatacagcc ttgctgaaaa tttttgcagt agcaatggct acacacttcc ccgtgcagat
     9781 catttaaacc atagtcgttc ccgaggcatc gggtcactgt acagtgaatg gggagatatg
     9841 gggcattaca cgactgaagc tggttttcaa tcaaatatgt attggtcatc tagtcccgca
     9901 aactcaagcg aacaatacgt agtttccctg gcaacaggtg atcaaagcgt atttgaaaag
     9961 cttgggtttg cttatgcgac atgttataaa aacctgtgat tttccgttat aatttcttaa
    10021 agagtgctcc attcggagca ctctttttta cattccctct atagagcttg cccttctctc
    10081 acagaaaatg aataaataaa actgctgcac cgcaagaatc gcgttcgatg tgactatgat
    10141 cgtatccggt gagtacagct ttgatccaaa ggctggtttt ctgtgatgat tgaccagcct
    10201 cagagtattg atgacaccac cgttcataac agatcggcat tactatgcat aagtactgtc
    10261 tcatattctc gccaaaacac tttttaatgc ctttgcgaat tcatccagac ttatattgtc
    10321 aattttttgc ccaacgacga ctgactccga aacatcggcc attgcgggcc atcgcgtttt
    10381 gtaatcagca taaattgcaa caacgggagt attataggca cttgccatat gtaatattga
    10441 ggtgtcagga gttattgcga tataagcatc ttttatgatt gctgctgaac gtttaatcga
    10501 gggtgataag gataaacggt agacattatt ataacaatcc actaataccc tggctttatc
    10561 ttcccctttt ggtccatgaa cgatcactat tggtatatct gtttcacttt gaatttttgc
    10621 tattagattt tctgcaatcg ataatgaaaa tgtacgttcc tgcgagctac cttctaaatt
    10681 aagcgcaatg taagatccta acgatcgcat ttcctcgcgc acctcatcga gtacatcctc
    10741 acttagtggc aattcatata ttggcctgac tgctggaaaa cccgcctcac gcatcataaa
    10801 tgcccatgtc atgggtacag gagcccggag attctgatcc atccgggacg cgttcttgca
    10861 caaaggggag tagcacttca tggttaaacc aacaacctga aaattcgttt ttgctttcaa
    10921 ctgactgata aatatcatcg ttttcaggtt ctttttacgc atcgcctcta tacaaagatc
    10981 cggcgtaccg tatttctgtg ttatgttctt tgctaaatct tttatttctt ttaatgttgc
    11041 gtgatcctgc atagtcattg tgactaatgt taatttagtc ttttcaagtt taagtgcatt
    11101 aaacacttct aaattaattg tcgacgtaac aattaaaaga tgcttaattt tatgcaattc
    11161 aagcgcccga ataacaggaa agatggccat agcatcgcca atctggtcgg gaatatggat
    11221 gacaacaaag tctgtttttt caatattgaa attataagct ttataatcgt agtaactaaa
    11281 tgcaatacgt ctcaacaatg atgctaaaaa catacctaac ctcgcctccc tactggttat
    11341 aatgcaatgc agtctatcag actcatcagg gtgccatttt gtgcatatgc ggacttttat
    11401 gtttcatatc tctaacctgt ggctcctctg cttaatcctt aaacaacacc agcaactcct
    11461 gcgctttcat cttccatcga atttttcatg ttgccgctaa tcagccataa aatcatttgc
    11521 agatgcgctc tgtcgaggta gtctcataag gttcgtttat agatcgacgg caatgtgagt
    11581 taccttttcc atactaatta taaaaagaca gtacaaacag gatcattatg gactccacgc
    11641 tcatctccac tcgtcccgat gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt
    11701 taggcagctt cgctggtgcc gtcgtcgact ggtatgattt tttactctat ggcatcaccg
    11761 ccgcactggt gtttaatcgc gagtttttcc cgcaagtaag cccggcgatg ggaacgctcg
    11821 ccgcatttgc tacctttggc gtcggattcc ttttccgtcc gctcggcggt gtcattttcg
    11881 gtcactttgg cgaccgactg ggacgtaagc gcatgttaat gctgaccgtc tggatgatgg
    11941 gcatcgcgac agccttgatt ggtattcttc cttcattctc gaccattggg tggtgggcac
    12001 ctattttact ggtgacgctg cgtgccattc agggatttgc cgtcggcggc gaatggggag
    12061 gcgcggcgtt gctttccgtt gaaagtgcac caaaaaataa aaaagccttt tacagtagcg
    12121 gtgtacaagt tggctacggt gtaggtttac tgctttcaac cggactggtt tcattgatca
    12181 gtatgatgac gactgacgaa cagtttttaa gctggggctg gcgcattcct ttcctgttta
    12241 gcatcgtact ggtactggga gcattgtggg tgcgcaatgg catggaggag tccgcggaat
    12301 ttgaacaaca gcaacattat caagctgccg cgaaaaaacg catcccggtt atcgaagcgc
    12361 tgttacgaca tcccggtgct ttcctgaaga ttattgcgct acgactgtgc gaattgctga
    12421 cgatgtacat cgttactgcc tttgcactta attattcaac ccagaatatg gggttaccgc
    12481 gcgaactttt cctcaatatt ggtttgctgg taggtggatt aagctgcttg acaattccct
    12541 gttttgcctg gcttgccgat cgttttggtc gccgcagggt ttatatcaca ggtgcgttaa
    12601 tcggaacgtt gagcgcattt cctttcttta tggcgcttga agcacaatct attttctgga
    12661 tagttttctt ctccataatg ctggcaaaca ttgcgcatga catggtggtg tgtgtgcaac
    12721 aaccgatgtt taccgaaatg tttggtgcca gttatcgcta tagtggtgct ggagtcggtt
    12781 atcaggtcgc cagtgtggtt ggcggtggat ttacaccttt tattgccgct gcactcatca
    12841 cttactttgc cgggaactgg catagcgtcg ccatttattt gctggctgga tgtctgattt
    12901 ccgcaatgac cgctttgttg atgaaagaca atcaacgcgc ttgatagtct ggcgaagatc
    12961 atccgatctt cgccttacac ttttgtttca cattcctgtg acatactatc ggatgtgcgg
    13021 taattgtatg gaacaggaga cacacatgaa taataagggc tccggtctga ccccagctca
    13081 ggcactggat aaactcgacg cgctgtatga gcaatctgta gtcgcattac gcaacgccat
    13141 tggcaactat attacaagtg gcgaattacc tgatgaaaac gcccgcaaac aaggtctttt
    13201 tgtctatcca tcactgaccg taacctggga cggtagcaca accaatcccc ccaaaacgcg
    13261 cgcatttggt cgttttactc acgcaggcag ctacaccacc acgattactc gccctactct
    13321 ctttcgttcg tatctcaatg aacaacttac gttgctgtat caggattatg gtgcgcatat
    13381 ctcggtgcaa ccctcgcagc atgaaatccc ctatccttat gtcatcgatg gctctgaatt
    13441 gacacttgat cgctcaatga gcgctgggtt aactcgctac ttcccgacaa cagaactggc
    13501 gcaaattggc gatgaaactg cagacggcat ttatcatcca actgaattct ccccgctatc
    13561 gcattttgat gcgcgccgcg tcgatttttc cctcgcgcgg ttgcgccatt ataccggtac
    13621 gccagttgaa cattttcagc cgttcgtctt gtttaccaac tacacacgtt atgtggatga
    13681 attcgttcgt tggggatgca gccagatcct cgatcctgat agtccctaca ttgccctttc
    13741 ttgtgctggc gggaactgga tcaccgccga aaccgaagcg ccagaagaag ccatttccga
    13801 ccttgcatgg aaaaaacatc agatgccagc atggcattta attaccgccg atggtcaggg
    13861 tattactctg gtgaatattg gcgtgggacc gtcaaatgct aaaaccatct gcgatcatct
    13921 ggcagtgcta cgcccggatg tctggttgat gattggtcac tgtggcggat tacgtgaaag
    13981 tcaggccatt ggcgattatg tacttgcaca cgcttattta cgcgatgacc acgttcttga
    14041 tgcggttctg ccgcccgata ttcctattcc gagcattgct gaagtgcaac gtgcgcttta
    14101 tgacgccacc aagctggtga gcggcaggcc cggtgaggaa gtcaaacagc ggctacgtac
    14161 tggtactgtg gtaaccacag atgacaggaa ctgggaatta cgttactcag cttctgcact
    14221 tcgttttaac ttaagccggg ccgtagcaat tgatatggaa agtgcaacca ttgccgcgca
    14281 aggatatcgt ttccgcgtgc catacgggac actactgtgt gtttcagata aaccgttgca
    14341 tggcgagatt aaacttcccg gccaggctaa ccgtttttat gaaggcgcta tttccgaaca
    14401 tctgcaaatt ggcattcggg cgatcgattt gctgcgcgca gaaggcgacc gactgcattc
    14461 gcgtaaatta cgaaccttta atgagccgcc gttccgataa taagttctga accttcttat
    14521 cagaaataga tgtgaaggag taagtaagac acctggcaaa tagcctgcaa taatcgtggg
    14581 ctgtttgctt ccttgggcgg atacg
//
LOCUS       DAZFYC010000124        14490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFYC010000124 DAZFYC010000000
VERSION     DAZFYC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.124"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..605
                     /locus_tag="ACHKW5_004120"
     CDS             39..605
                     /locus_tag="ACHKW5_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7513399.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            656..3115
                     /gene="ybgQ"
                     /locus_tag="ACHKW5_004121"
                     /pseudo
     CDS             656..3115
                     /gene="ybgQ"
                     /locus_tag="ACHKW5_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3130..3861
                     /gene="ybgP"
                     /locus_tag="ACHKW5_004122"
     CDS             3130..3861
                     /gene="ybgP"
                     /locus_tag="ACHKW5_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7513400.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3858..4919
                     /gene="ybgO"
                     /locus_tag="ACHKW5_004123"
     CDS             3858..4919
                     /gene="ybgO"
                     /locus_tag="ACHKW5_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7513401.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5071..6117
                     /gene="abrB"
                     /locus_tag="ACHKW5_004124"
     CDS             5071..6117
                     /gene="abrB"
                     /locus_tag="ACHKW5_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO7513402.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6114..6905)
                     /gene="nei"
                     /locus_tag="ACHKW5_004125"
     CDS             complement(6114..6905)
                     /gene="nei"
                     /locus_tag="ACHKW5_004125"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO7513403.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="ACHKW5_004126"
     CDS             complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="ACHKW5_004126"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO7513404.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="ACHKW5_004127"
     CDS             complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="ACHKW5_004127"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO7513405.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="ACHKW5_004128"
     CDS             complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="ACHKW5_004128"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO7513406.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="ACHKW5_004129"
     CDS             complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="ACHKW5_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO7513407.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10284..11765
                     /gene="dtpD"
                     /locus_tag="ACHKW5_004130"
     CDS             10284..11765
                     /gene="dtpD"
                     /locus_tag="ACHKW5_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO7513408.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11807..13225)
                     /gene="phrB"
                     /locus_tag="ACHKW5_004131"
     CDS             complement(11807..13225)
                     /gene="phrB"
                     /locus_tag="ACHKW5_004131"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO7513409.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13222..13731)
                     /gene="ybgA"
                     /locus_tag="ACHKW5_004132"
     CDS             complement(13222..13731)
                     /gene="ybgA"
                     /locus_tag="ACHKW5_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO7513410.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13882..14490)
                     /locus_tag="ACHKW5_004133"
                     /pseudo
     CDS             complement(13882..14490)
                     /locus_tag="ACHKW5_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3503 a         3871 c         3500 g         3616 t
ORIGIN      
        1 ccgatatact tttttctttt ctgttaaagg actccgttat gtttaaagga caaaaaacat
       61 tggccgcact ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat
      121 ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg
      181 atattgataa agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc
      241 acagcgatgc cgttgctgtc gacctgcgct tagtcaactg tgatctggaa aactccagca
      301 acggttccgg tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca
      361 ccggcgcaga tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac
      421 gtttaatgaa taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc
      481 tggctccgac ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc
      541 aggctacacc tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata
      601 aataagtatt actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga
      661 caccgtgaat atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc
      721 attggcggtc gaattcaacc ttaatgttct cgataaatca atgcgcgacc gcattgatat
      781 ttcattatta aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt
      841 aaataataac caaatcagta acgggcaaaa gattaactgg cacaaaaatg acgataaaac
      901 cattccgtgc atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca
      961 gtcgttacca ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa
     1021 tttcgatcaa gccaatcagc aactaaatat caccattccg caagcctggc tggcgtggca
     1081 ctcagaaaac tgaaccccac cctccacatg gaaagaaggt gtcgccggtg tcctgatgga
     1141 ttacaacttg tttgccagca gctaccgccc acaggatggc agcagcagca ctaatcttaa
     1201 cgcctatgga acagccggaa tcaacacggg ggcatggcgc ttacgcagtg attatcagtt
     1261 gaatcagact gatagcgatg ataaccatga acagtcaggc gaaatatcac gtacctatct
     1321 ttttcgcccg ttaccgcaat taggttcaaa attaaccctc ggcgaaacgg attttagttc
     1381 caatattttc gacggttttt cttataccgg cgcggcactg gcaagtgacg atcgaatgtt
     1441 gccatgggaa ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac
     1501 ggtgacgatc agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt
     1561 tatcattgac gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga
     1621 agatggtcgg gtgaacaatt tccaggtttc ggcagcatcg acgcccttcc tgactcgtca
     1681 gggacaggtt cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac
     1741 tgaaaatgaa acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct
     1801 gtacggcggc ctgctgcttt ctggtgatga ctaccattct gccgcaatag gtattgggca
     1861 aaatatgctg tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta gccattttga
     1921 tactcagcag gacgagcggg gcttaagcta ccgttttaat tacagcaaac aagtggatgc
     1981 cactaacagc acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta
     2041 cgccaactat ctggatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat
     2101 cagcttatct gtgggccaac cgattacccc actaaacctc aatctttacg ccaacctgct
     2161 acatcaaacc tggtggaatg cagacgcctc cacgaccgcc aacatcacgg caggttttaa
     2221 tgttgatatt ggtgactgga gagatatctc tatttcgacg tcattcaata caacccatta
     2281 cgaagataaa gatcgcgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg
     2341 tggtcgggtt ggctatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg
     2401 gaacgatacg ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg
     2461 tcctgacaat ggagcccagg tgagcggtaa ctatcagcac ctgagttcag cgggtgagtg
     2521 ggatatttct ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg
     2581 ttctttcacc gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc
     2641 acgcctgatg gtcagcaccg atggcgtggc agatattccg gttcagggca atctcgacta
     2701 caccaaccat tttggcattg cggtggtgcc gttgatttcc agttaccagc cttccaccgt
     2761 ggcggtgaac atgaatgact tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga
     2821 aacgtggatt gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt
     2881 taacgtcatc attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca
     2941 ggatgacagc ggcattagcg tggggatggt tggcgaggaa ggacatgcct ggttaagcgg
     3001 agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat agccagcatt gctcgctcca
     3061 tcttcctgaa catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag
     3121 gaaatgaaaa tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc
     3181 agccacgctg cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct
     3241 accagtctgc gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc
     3301 gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt
     3361 ctggaaccga aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta
     3421 ccgggcgatc gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa
     3481 aaaaacagcg accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg
     3541 cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc
     3601 caacagggca accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac
     3661 ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt
     3721 agtacggtca acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg
     3781 gatgattacg gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa
     3841 gccgtggagg cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat
     3901 ttctgccgtt gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg
     3961 tagaaaaatc agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata
     4021 agatttggga atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca
     4081 attttgaaag tgagcatgtt tatgcctggg taaaccccta cccgggggtg caggatcgct
     4141 attatcaact cggcgtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc
     4201 gcatcgacac taaccagtgt attgacagta agaacattga catttatacc cctgagcaga
     4261 ttatcgcgat gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt
     4321 cacgtacctt tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg
     4381 attatcaaag tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta
     4441 acgaagatcc tactgcccaa aacctgaaat atcatattac cggtctggaa aacattcgcg
     4501 tgctggattg cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta
     4561 aatttaattt gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga
     4621 caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg
     4681 aagaaacgtt ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt
     4741 tgctggatga aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca
     4801 ccagcgacat gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta
     4861 cccccatcga ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat
     4921 ttgccatgat gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc
     4981 ccttttcctt gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac
     5041 accaaagaga aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt
     5101 gtgctatcac ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta
     5161 cttgggccga tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc
     5221 cgctccgctt ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact
     5281 ggttcgattc tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg
     5341 acgctgcttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga
     5401 aataccggtg cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa
     5461 gattacggcg cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc
     5521 gcgggggcag ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac
     5581 cagcaaatcg tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca
     5641 gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg
     5701 atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact cccggaatgg
     5761 ttgctggcga tggcgtatat ggcaattggt tggcggattg gtcttggttt cgataaacaa
     5821 atcttactgc gggcattgcg cccactgcca caaatcctgc tgtcgatttt tgctctgctg
     5881 gctatttgtg cgggtatggc gtgggggctg acccggttta tgcacattga ttttatgact
     5941 gcctacctcg ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc
     6001 aatgccgata tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg
     6061 acggggccag ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc
     6121 tggcagcctg gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg
     6181 ccacagcgtt cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca
     6241 tgatgcttat tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt
     6301 aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca
     6361 gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac
     6421 gcctgatcga gcagtaatcc tgcaaactga cggttacgaa agcgcggtga caataaccgt
     6481 tcctttacca cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa
     6541 aacggatgcg tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc
     6601 agaatggttt tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct
     6661 tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaag
     6721 tcgttggaaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga
     6781 ccgataagtt gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt
     6841 ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca
     6901 ggcatggtga tatccttttt tcattcttta ttaatcgggg ttatgctgcg acaacaatct
     6961 ccttttcggc aaatgtagag cgtagtcggc gggcgaacgc cagtgcgtgc tcgccgtcgc
     7021 catgcaggca gactgtttgc gccgtgaccg ttgcccattc gccggtgata cttttgactc
     7081 tgccgtgttg caccatctcc agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg
     7141 cgcctcgctg acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca
     7201 cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca
     7261 gcccaacgag aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca
     7321 gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta
     7381 cgccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt
     7441 aaacggtttc tggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac
     7501 tcgggtgagc gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatga
     7561 tttgcgcatc gcctgcatga aatccacagg caatattggc agaggaaacc agcgttaata
     7621 gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc
     7681 agccgccacg ctaattgttc gaaataacgt tgctgatctt gccgcgcttt cagtgcctct
     7741 tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac
     7801 atatcagcct caatgataca ggcaatacgc gggtaaccac cggtggtctg tgcgtcgttc
     7861 atcaacacaa tcggctgccc gttatgcggc acctgcacca cgcccggtaa caaaccgtga
     7921 gataacagtt cgcgatcggt gatgcgtttt aaaatttgcc cctgtaagcg atagcccatt
     7981 cggttacttt gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga
     8041 tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt
     8101 ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca
     8161 tccttcagta aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac
     8221 cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc
     8281 tgcgggcgtt taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag
     8341 acggcattat catccagccg cgcttcgcaa ccggcacccg tcagagcaaa ccacccatca
     8401 gtttcgaact caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca
     8461 ttacccacca gcaggttagc aatgcgtaac gcaggcatat ccagtgcgcc gcagtggctg
     8521 atacccgact ggcgaaaacc gtgacgaccg ccatcttgca cagtggtata catgcccgca
     8581 cgaataatct tcagcatact ccctcctttt gcggtacaaa gcgcacgctg tctcccggac
     8641 gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca
     8701 actgccaccc acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga
     8761 cagaacctgc cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg
     8821 gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca
     8881 cggaggagtg caattcaaca acctgttttt cgctcaaccc gcaatgcgcc gcgaccaccg
     8941 ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag
     9001 gttccagggc ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca
     9061 gcgactcagg attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca
     9121 cattcggcat atccaccaga cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg
     9181 tcaccggcgg ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc
     9241 gttgcacttt cgatccctct gattattcgt tatgcaggat taggtatatc aataaaggtc
     9301 acatcaagat cggtattttc attcagccac tcactcaatg cgcgaatacc accgcgttca
     9361 gtggcatggt gacctgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc
     9421 tgttcagaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata
     9481 aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg
     9541 ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccagtccc
     9601 ggcacaggca tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg
     9661 atccccagta acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc
     9721 aaatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc
     9781 attccgcgaa tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca
     9841 tccgcgccca gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca
     9901 attttttgca ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc
     9961 gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg
    10021 ccttccgtat tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag
    10081 ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc
    10141 atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata aataatcaga
    10201 tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg
    10261 ttgccgtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact
    10321 atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga
    10381 ttctctatct caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg
    10441 cctactgttc gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc
    10501 tcggcaatcg catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc
    10561 tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg
    10621 gctatggtct gtttaaatcc aatgtcagtt gcctgctcgg cgagctgtat gagccaaccg
    10681 atccgcgtcg tgatggcggt ttctcgctga tgtatgcagc gggtaacgtg gggtctatta
    10741 tcgcacctat cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc
    10801 tggcggcggt gggtatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca
    10861 cacatacccg cggcgttaat aaaaaagtac tgcgtgcgac aaactttctc ctgcctaact
    10921 ggggatggct gctggttctg ctggtggcaa cgcctgcgct gattaccgta ttgttctgga
    10981 aagagtggtc ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa
    11041 aaatttatcg caaagcagaa aaccagaaac agcgtaagga gctggggctg attgtgacgc
    11101 tcaccttctt cagtatgttg ttctgggcct tcgcacaaca gggcggcagc tcgattagcc
    11161 tttatatcga ccgcttcgtt aaccgcgata tgtttggtta taccgttccg actgctatgt
    11221 tccagtcgat taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta
    11281 aagaaagcgt cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggtctcg
    11341 gcctgatgag cgccgggttc tgcattctga ccttaagcgc ccgctggtcc tcaatgtatg
    11401 gtcactcttc tctgccactg atggtattag gcctggcggt gatgggcttt gcggaactgt
    11461 ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga aatccccggt gtgaccggcg
    11521 tattaaccgg catttatatg ctgctttccg gcgcgattgc gaactatctc gcgggcgtga
    11581 ttgccgatca gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca
    11641 atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg
    11701 tactgatgat ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggaat
    11761 cttaataggc cagcaccata cctgataaaa cgctcgctgc gttttatcag gctcctttcc
    11821 tcgcttcttc gtacgctgcc agcgttcgca gccttgcctc tttgtgatca actatcggtt
    11881 gcggataatc cagcatcaca cctgctttct gcgcccactt ccacggctca tgcaccgctt
    11941 tccctggtac atcgcgcagc tccggtaacc atcggcggat aaactcgccc tcacggtcaa
    12001 atttctcgcc ctgagttatc ggattgaaaa tacgaaaata cggcgcagca tcagtacccg
    12061 tagaagccgc ccactgccag ccaccgttat tagctgccaa atcaccatca atcagctgcg
    12121 acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc agaaaactgg
    12181 ctgtaatcat ccgtagccgg ttatgcatcc agccagtact gttaagctga cgcatagcgg
    12241 catcaacaat cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc
    12301 tttgccactg cacacgatcc gtccaggcaa taaacggaca atgtttacac aacgaggggt
    12361 aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc cagacactac
    12421 cggccccacc gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag
    12481 gcgataaccc gcccgttgcc aggctggcgg acaaacgact ggtgccttcc actgccggaa
    12541 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
    12601 tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa agactgacga ggataattca
    12661 gcgtaatgga tggcgcgggc tctatcgatc cgctactacg aacttttggc gcagcgacac
    12721 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
    12781 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
    12841 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
    12901 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
    12961 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
    13021 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
    13081 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atataacgcc agcacgcgtg
    13141 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcctggc
    13201 gaaaccagac cagatgggta atcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
    13261 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg ccatatagtg
    13321 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
    13381 cgccgccaac tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
    13441 catcaaaacg ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa caattacgcg
    13501 ttggtagtac tggttgtaaa aatcatccag attttgccac tggtgaatat cagccacgaa
    13561 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
    13621 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
    13681 aagggcgaag atacggatta gggtgctgtc atcaaatcgt tgctgattca ttactgctcc
    13741 tgaacggttt acaggagtaa gtgtagacgt tgagaaatga tatttgtgaa gttaatcctg
    13801 ttgtagcagg acaaatttac gaaagcctgc tccccgcaag gactgacgtc aggtagtttc
    13861 taaccatagc tgccctttct attcaaacgt gaaatcaata agctcatcag ggacatcgca
    13921 aacaatatga agacgggttt cttctctgcc gtgactcttt tcactaattg cgtaactgtc
    13981 atgcttggga ttatttaatt ctttcagcgg aaatttttcc tcgaaggctt tatttagccg
    14041 cccctggttt ccttttacag cgaataaata atcacctccc tgtttttgta tcttctctgc
    14101 aatatctttc tggcaaccca tcgcatcggt tgtgatgatt tttcctttaa tatccagcat
    14161 gttaagaagt tcagggatag ctgtgatctc attggatttc ttatccgtct tgatctgccc
    14221 gatgaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc ccctgcggcg
    14281 actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt ctttatcatt
    14341 tgatgaatgg caatcacgca tccagttaat aaagcactcg tgaaattttg caggactgat
    14401 acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa aatcaccata
    14461 ttgcttcaag aaatcgagat gtgtttcccc
//
LOCUS       DAZFYC010000125        14085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFYC010000125 DAZFYC010000000
VERSION     DAZFYC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.125"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1113)
                     /locus_tag="ACHKW5_004134"
                     /pseudo
     CDS             complement(1..1113)
                     /locus_tag="ACHKW5_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611680.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1156..1320)
                     /locus_tag="ACHKW5_004135"
                     /pseudo
     CDS             complement(1156..1320)
                     /locus_tag="ACHKW5_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(1816..2319)
                     /gene="cdiI4"
                     /locus_tag="ACHKW5_004136"
     CDS             complement(1816..2319)
                     /gene="cdiI4"
                     /locus_tag="ACHKW5_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDI system immunity protein CdiI-o11"
                     /protein_id="HFO7513411.1"
                     /translation="MSFNKDQDYWANIFVTPDFLSVETYSGLGMTGRDPLFSPRLLQP
                     DVDDKSLGEAILQALSDSRTLDVLEDRVAFFDLEKSKEQYAAWIATLMEKYGYRTKRA
                     LFKNMKKVGIHLVNDVITIRPSFHEKLEAWSGNRINESDYVVLPADSSPTEIGSGLRL
                     ALSRCKG"
     gene            complement(2319..3209)
                     /locus_tag="ACHKW5_004137"
                     /pseudo
     CDS             complement(2319..3209)
                     /locus_tag="ACHKW5_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245723.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3208..3426)
                     /locus_tag="ACHKW5_004138"
     CDS             complement(3208..3426)
                     /locus_tag="ACHKW5_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513412.1"
                     /translation="MINVLFALFSILAGGILAEIAYIFLIIIEYAMVGSFNFDLTSAW
                     LYFKAAGVEGGIMGIGISLFRYFGVKGF"
     gene            complement(3423..5453)
                     /locus_tag="ACHKW5_004139"
     CDS             complement(3423..5453)
                     /locus_tag="ACHKW5_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7513413.1"
                     /translation="MNIRIRSGMLPCMDISALNTTNDIEKLRAMALAMVQKVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDVDIAELTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPAHLPRVEKIIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNCYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGEHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELISRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPELANIIGHHAGIDDNTAAKAIAHAILGGVTAALQGNSAAAGAIGAGTG
                     EVISSAIAKSLYPDVDPSKLTEDQKQTVSTLATLSAGMAGGIASGDVAGTAAGAGVGK
                     NIAENNLVGGTESEQSKAAQTHGADVLSCNDAPGGEACKRGEAVNKAYAGALATGSVA
                     LLPGGAQAMWALGTGANAGIGYMVDSSVDPENAIIAGWVNVITMGQGWKGTVGWNAAG
                     GALGNWIDDKDPLSGALINGTGSGIGYGVGKGLSWGVNAGENWWKGGWDPKFNSELRS
                     VTKVKGDYGLSKEMKPSKVPSSFGDVGGSAFSEITGKGIEKISSSNANGDKK"
     gene            complement(5460..5852)
                     /gene="tnpB"
                     /locus_tag="ACHKW5_004140"
     CDS             complement(5460..5852)
                     /gene="tnpB"
                     /locus_tag="ACHKW5_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258751.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7513414.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDTLTQYIQDELRSTWHEGAAFVFV
                     NKDRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHGLTKWVWQNEPELRPENTQNTLLTH"
     gene            complement(5839..6168)
                     /locus_tag="ACHKW5_004141"
     CDS             complement(5839..6168)
                     /locus_tag="ACHKW5_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7513415.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            complement(6258..6714)
                     /locus_tag="ACHKW5_004142"
                     /pseudo
     CDS             complement(6258..6714)
                     /locus_tag="ACHKW5_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(6738..6994)
                     /locus_tag="ACHKW5_004143"
                     /pseudo
     CDS             complement(6738..6994)
                     /locus_tag="ACHKW5_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046881606.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(7355..8263)
                     /locus_tag="ACHKW5_004144"
     CDS             complement(7355..8263)
                     /locus_tag="ACHKW5_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7513416.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVASPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            8390..9748
                     /locus_tag="ACHKW5_004145"
     CDS             8390..9748
                     /locus_tag="ACHKW5_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7513417.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     VGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTQFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYATGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSIPDSLPHAGVV
                     NE"
     gene            9760..10788
                     /gene="gtdA"
                     /locus_tag="ACHKW5_004146"
     CDS             9760..10788
                     /gene="gtdA"
                     /locus_tag="ACHKW5_004146"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFO7513418.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEDQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRMGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFSSRVARTTD
                     STIYHVVEGSGQVTIGNEIFTFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            10804..11505
                     /locus_tag="ACHKW5_004147"
     CDS             10804..11505
                     /locus_tag="ACHKW5_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7513419.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDKQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVIPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            11514..12158
                     /gene="maiA"
                     /locus_tag="ACHKW5_004148"
     CDS             11514..12158
                     /gene="maiA"
                     /locus_tag="ACHKW5_004148"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFO7513420.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGQSLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            12173..13366
                     /locus_tag="ACHKW5_004149"
     CDS             12173..13366
                     /locus_tag="ACHKW5_004149"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFO7513421.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVRIETRQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGTQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTNRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            13716..13856
                     /locus_tag="ACHKW5_004150"
     CDS             13716..13856
                     /locus_tag="ACHKW5_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513422.1"
                     /translation="MPPHKIRQEWLALDETAEVILQHSITLQSADLIRHSFIWRNRSI
                     CL"
BASE COUNT         3284 a         3712 c         3454 g         3635 t
ORIGIN      
        1 cggtcttagt gcgctgtaat atccaacgat atagtccgtt attgcgtgag ctgcatcgct
       61 gaagcttaca tagcccgtcg ctggtaccca ttcgttcttc agactcctga agaagcgctc
      121 cattgggctg ttatcccagc agtttccacg ccgactcata ctctgcctga tccggtatcg
      181 ccacagtaac tgccggaact gcctgctcgt ataatgactg ccttgatcgc ttggaacatc
      241 accccgacgg gcttaccacg ggtttcccat gccatttcca gtgctttcat ggtaagcctg
      301 ctgtccggcg agaacgacat ggcccagccc actggttttc ttgcgaacag gtcgagaaca
      361 acgacgaggt acgcccggcg cttacccgtc cagatatagg tcacatcacc gcactacacc
      421 tgatttggct cggtcacggc gaactgccgt tcaaggtagt tagggatagc aacatgttca
      481 tgaccaccac gtttataccg gtgagtcggc tgctgacagc tgaccagccc cagctctttc
      541 atgagcctgc caacaagcca gcatcccatc tggtagcctc tccgggttgc cattgtggcg
      601 atgcttcttg ctccgaccga accgtggctg atgccatgta gcttaagtac ctgactgcgt
      661 aatacagccc gtctgccgtc tggtttttca ggacggtttt tccagtatct gtagctgctg
      721 cgatgaaccc cgaacacatg gcagagtgtg accacaggat aatgcactct gagtttcccg
      781 attatcgaga actgttcagg gagtctgaca tcaagagcgc ggtagccttt ttaatatttc
      841 attctccatt tcaatgcgtt gtagcttttt cctcagctca cgtatttcga tttgttctgg
      901 tgttatcgga gaggtttttg gtgttttgcc ctgacgctca tcacgcagtt gtttgaccca
      961 tcttgtcatt gtggaaaggc caacatccat agctttggcg gcatctgcca ccgtgtagtt
     1021 ctggtcaaca accagttgag cggattcgcg tttaaactct gcgctgaaat ttcttttttc
     1081 attggagcac ctgtgttgtt ctgaggtgag catctcacct ctgttcaggt ggccaaattc
     1141 agtaaaccac tacacatccg tttcaggctc agcaaaagtt aaggtttgtt gtagcaaggt
     1201 cgtaaattca cggtatcgct gatcccactt ggaagctttg taatttttat cttttgcaag
     1261 agtatcaatt aattctctga gagtcagctt gcctgcttcg acactatcca aaagagtcat
     1321 cgtgactttt cctctactgt aacattactt actcttgaat tgttatgtgg tctaaattgc
     1381 cacggataac cgatataata tacattgctg tcaatgtcag atgaaaagtg ctccactaac
     1441 gtcctagtat tgatgcactg ttttactcag gattagcctc tgctataact ccggcctttc
     1501 atttctgtct ggttcgattc attcctcctt tattttaacg aggcctgaga ggtgtaagat
     1561 ctggtccaga atagcttctg gtcagcatca tcgtcaaacg tttgagccca ctatgacgca
     1621 attacccgac tcagcacttc atagccagcc tcgggtacca gctaagctgt tgcccttgtg
     1681 caacaaaata aatcaaccat cgtcagacca cgatcgttcg tatgcgccgc cgatgacgcg
     1741 cgcccgaaaa acgcgtcaag atgatatgct tggaagcaca caaaaatccc gccagtgatg
     1801 ggcgggatat ctaggttaac ctttgcagcg actcaaggcc aggcgaagac ctgagccgat
     1861 ttcggttggg gaactgtctg caggaagcac cacatagtca gattcattga tcctgttacc
     1921 actccatgct tccagctttt catggaatga aggccggatt gtgataacat catttaccag
     1981 atggatacca acttttttca tgtttttaaa taatgcacgt ttagttctat aaccatactt
     2041 ctccatcaga gtagctatcc aagctgcata ttgctcttta cttttttcaa gatcgaaaaa
     2101 agctactcga tcttccaaaa catctagtgt acggctgtca gacaatgcct gtaggatcgc
     2161 ctcacctagg cttttatcat ctacatcagg ctgtaataaa cgtggtgaaa acaatggatc
     2221 tctgcctgtc attcctaatc ctgaataagt ttcaacactc aaaaaatctg gtgtaacaaa
     2281 tatattcgcc cagtaatcct gatctttatt aaaagacatt atttcacctg cgttaccgtc
     2341 actttgacgc cctgagattt accgtactca atagccctgt ttatttctgc ccactgtgtt
     2401 ttagttgtgt cagcaggaat agcgagctgt atctccctgt tagagatcat tgatgaagtt
     2461 aattctcgcc cagagagtgc atattcttta aacttagccg cagcatcgat gtttcctttt
     2521 atcgaactgt aaacctgatt cggattagcc aatttcgcca tagtttgcgt atccattgat
     2581 tttacactgg tagcagtttt ggtagcacca tcatagtaat caaaggtctt aaagttcttt
     2641 ggtaaacggg catcagcgga aagagatttc ccaacataat cctcccacag cataccctgt
     2701 ttcatatttc cctgttccca ctgcattcca acttctgttg ctttgaccgt cgcagtcgct
     2761 gtattgcgac tcatgaatga tatagccgct ttggttttat cttttaccag taaacctccg
     2821 ctgaccagca tggctgattt tcaggatcgg aagcaaacac tgcaaacttc gtccattcag
     2881 ggctgtttac atctattttt gccagatccc ggccattcca ctgctgaaca taggctttgg
     2941 cttcatcact ggtcattact tccgcaccaa tgttacccag ccaaccaggg cgctgcgtca
     3001 tatcatatcc gcccttcgca acttccttat caataatctg ggctcccagc ggattgtctt
     3061 tcagcttctg atcgatttct gcgctctgtt catcactgat tttcttccat ttatcgataa
     3121 cattctgaca gttccccccg gaattccgat agcaccttgt caaaagtcag agactgctct
     3181 tcactaaggt aattattctc aacatcatta aaatccttta actccaaaat atcgaaatag
     3241 agaaattcct attcccatta tacctccttc tactcccgcg gccttaaagt acaaccatgc
     3301 tgatgtcaag tcaaagttaa aactaccaac cattgcgtac tcaataataa ttaaaaaaat
     3361 gtaagcaatc tcagctaaaa tcccccctgc caaaattgaa aataacgcga aaagaacatt
     3421 gatcatttct tatctccatt agcatttgaa gacgaaattt tttcgattcc ttttcctgtt
     3481 atttcggaga aagcagaacc acctacgtca ccaaatgagc taggaacttt gcttggtttc
     3541 atttcttttg aaagaccata atccccttta actttagtaa cacttctcaa ctcagaatta
     3601 aacttcggat cccaaccacc tttccaccag ttttcccctg cattaacacc ccatgaaagt
     3661 cctttgccaa ctccataacc aatacccgaa ccggtaccat tgataagggc acctgacaat
     3721 ggatctttgt cgtcaatcca gttacccagc gcaccacctg ccgcattcca cccaacggtt
     3781 cctttccagc cctgccccat ggtgattaca ttgacccatc ctgcaatgat tgcattttca
     3841 ggatcaactg aactatctac catgtaacca attcctgcat ttgcacctgt gccaagagcc
     3901 cacatggcct gagcaccgcc aggtaagaga gctacactac cagtggccaa cgctccggca
     3961 tacgctttat tgactgcttc tcctcgctta caggcttcac cgcctggagc atcgttacag
     4021 gaaagtacat ctgcgccatg cgtctgagca gctttgctct gctcggactc tgtgccacca
     4081 accaagttat tctcagcaat gttcttccca acaccagccc ctgcagctgt gccagccaca
     4141 tcgccactgg caatgccgcc agccataccc gctgacagcg ttgccagcgt gcttacggtt
     4201 tgcttctgat cttctgtcag tttcgacgga tctacgtccg ggtagaggct tttcgcaatg
     4261 gctgacgaga tcacttcacc agtacccgca ccaattgcgc ctgctgccgc actgttgccc
     4321 tgaagggctg ctgtcacacc accgagaatg gcatgggcaa tggcttttgc cgctgtattg
     4381 tcatcaatac ccgcgtgatg accgatgata ttcgccagct ccggcacttt gtttgccaga
     4441 gtccggtaag ctttatgtcc gtcgacaacc agcgtcccgc cccagccctg aagccagttt
     4501 tcaggatact catggctacg tccggtccgg cagtcgaagc acacaactga cggtcccgtc
     4561 ctttctccac tgacgtatgc ccacagataa ccggagcagg atttaccgcc tttcttcgtg
     4621 ttcaggatct tcagggtagt ctcatctgca tgcaccaccg gacggcttat caattcgcga
     4681 tggagtaacg ccgcgagggg ggataatgcg gcccctgccg cgccaaccat gtcagccatc
     4741 gaactgacgg gcagtccgac atctgaacgg gcaaagacct gttgctggcg atacagaggc
     4801 aggtgctcac cgtatttatt gatgatcacc tgtgcgatga ctgatggctc aacggcactt
     4861 ttcgggagga tatgtgccgg catttcaccg ctgaacacct tctggcaaca gggacaactg
     4921 tattgcggac ggacataaca gttcaccaca aagtgagcgg gaatatactc cagcttttca
     4981 ctcaccgcat cacggatata gtgcagcggc tcgtcacatt cagggcaatg gtcagtgtcc
     5041 ggctggataa ttttttccac ccgtggaaga tgagccggta agggtttgcg tatcgggcgt
     5101 gaacgggacg ctttttcgtc ttcttcaggg gattgcggga gcagtttatc cagatgtgcg
     5161 gtgagctcgg cgatatcgac atcaacatcc tcttcgaaca gggagcgttg cataccagcc
     5221 agcgtttcac attttttacc gaagcgctgt tgacgaacca gtttcagcat ttcttccaga
     5281 agctggatgc gccggctttt ctccagtaat tcccgctctt tttcggcatt ctccgacatg
     5341 actttttgta ccatggcaag tgccatagca cgcagttttt cgatgtcatt cgtggtgttg
     5401 agagcggaga tatccataca ggggagcata ccactccgga tacgaatatt cattattttt
     5461 cagtgagtta gcagtgtgtt ttgcgtgttt tcagggcgca gttcaggttc attttgccag
     5521 acccatttcg tcaggccatg tcccttaacc tgctgccagt caacaccggc aatcagccag
     5581 ttaaactcgt cgggagtgag gtgccaggca gcatcatttg cacgtggcca gcggaagctg
     5641 cctttgtgaa gacggcgggt acacaaccac accccgtgtt tatcccaccg gagaactttg
     5701 atacgcgaac ggtctttgtt aacaaagacg aaggcggctc cctcatgcca tgtcgaccgg
     5761 agttcatcct gtatatactg cgtcagtgtg tcgatgcccc ggcgcatgtc aacaggttta
     5821 atcgcgatga agacattatc aggactgagc atatttgagc gccctgaaga catcggtaag
     5881 ttgtgacggc tgacagcaca tccgtactcc gccgggaagg aagagcgtga ctggctcatt
     5941 cgtgacgggc tgtggtacat cagcatgcag tggtggcgca atatgaacgg gaagaacggt
     6001 cggttcattt gcccggcgtt cagcttttgc gacatcctgc ggccattcgc gaagtgatgt
     6061 gaagggaata tcgttgagtt cacagtattg ctgacgtgta agaccactgg cgcgccaggc
     6121 agcaacgtgc tgccttttct gttcaatggt ccatcggggt tttgacatcg attatctccg
     6181 gtaagttggt gaatgaatgt cggtaatact aactgactga agatccctgt gaagatgact
     6241 tccccgtaac gacacagaat atcttccgca atacgctgac taccagacca gaacagaccg
     6301 tccagtgcgg tcaccagtaa tccctcctct agcgcatact taagtcattc acggagcgct
     6361 ttcactttcc gggcaatctg ctgccaaggc gatgatgtat ttcattccca accatccggc
     6421 gaatacggtg agacagatga taccggaatg tatccgcacg ccggaaagac tggccttcag
     6481 gctatacacg caaccaaagc gtttatcgtt aaacaccaca tttttctggc tgatatccca
     6541 ttcttcccaa agcacagcaa tcagctgtga cgtgcgggtt aaaagcagac gaaaaggcag
     6601 ttcaaaactg gtggtataat ccacattcag cagggcatta cgcagccgct ctcctgatcc
     6661 gacctctgcc tgccgacact cctcctgaac atcctgccac tgctggagaa ggcgtacaac
     6721 aacacgaaca ttctcagcgt atttttttga gtaaccaact gaaaaatggt gggaagatca
     6781 tttttcagtt gagaattccc caacggtcaa tccacccgct ttatcttgtt tttaactaat
     6841 gctgtctggt taaatgacgc ccgatgctgc taagcgtgag ggaagcgcca tttattacag
     6901 catttgttgc gtcaatcagt gatttttgat ggtagaaatg caaagtggcc agtatgtttt
     6961 taaggaattt ttggcataat gaacgtgcag gcatagtggt gatatccttg aaattgctag
     7021 cacaatcaat tagatcacat ctcactatgc ctgtcttgtt ttctgggaat tcctaatggg
     7081 ttgtccttcc aattggttct ttcgaggcct gctcagattc actcacatta tgacctgtat
     7141 actcactgaa acacctacag cttcttcatc aaatacatca gccgctttgt tacccacacg
     7201 actgctttga ctgctaccgg ctggaacgac agtcacctgg tgggattttc accccatgaa
     7261 agatcacgcc ttatcacagt gcacgattcc ccgaaaaatt acgcatcgtt gaattacagg
     7321 gaagtaacat cgtgcgaatg cccagttcag cacactaatt ctgctgtaaa aagtgcagaa
     7381 actgacttaa agcataagaa ggcgtttcgt tattgcgcca gaacacaccg acgctgcctt
     7441 tctgttctat ctttggcagg ttgagtatca ccagttgtcc ctggctggca taacgttgcg
     7501 ccaggcgtaa tgacaaaata gagatcatat cgctgctttg taacaggttg gtactgacgt
     7561 tcatcgacgc cgactcaatc gtgttttctg gcagcattac gccgtggtct accagcgcat
     7621 tatcaatact aatgcgaatg ggggtacccg tcggccagac gatccagcgc cagttggcaa
     7681 gatcattcca actgagggtg gcgtatttcg ccagtgggtg ctcagggctg gcgacaaagc
     7741 atactggctc ggtgtacagc acctgataat tcagtggcag ttgcaaggcg cgaccgccaa
     7801 ttcgcccaac gaccacgtcg acagaacctg ccaacagatc ttgcagcaat ggggtcatca
     7861 ccttctcttc gatattcagg tgtagagttg gcatcttcgt aagcaaatgc aaaatcgcct
     7921 gggatacgca gtcagtggcg acgggagaac agccaatttt cagactgccg accagtccac
     7981 cttgtttaaa acgggccagt tcatgttgcg atcgctccat gtcattgatt aaccgttggg
     8041 catgttgcaa taacaatttg ccgccttcag tcggacgtaa tcctttgctg tgccgctcaa
     8101 aaagtgtgat tccaatctca tcttcgagtt gtgacaacca tttcgaaagc gcaggttggg
     8161 tgatattcat cattcgtgcg acatgggtca gattgccctg ttcacccaac gccaccagca
     8221 tttcaaggtg atgtagtttt aatttttgcg cccagttcgc catttaccat aacctccagg
     8281 ttatctctct gcccgaaatg ccattgtaca tttcatctct ttaatcacaa tatcgcaaca
     8341 tatacaaaac aaaagaaaca tctaccttac ctgcactgag gcatttacta tgactcaacg
     8401 acgtgatcta caagcactta tcgatgctgc tccggtcggc aagatgcagt ggcgcgttat
     8461 catctgctgt tttctggtgg taatgctcga tggttttgac accgcggcta tcggatttat
     8521 cgcccctgat attcgcactc actggcaact caccgcaggc gatctcgccc cgctttttgg
     8581 cgcgggtctg ctggggttaa ccgcaggcgc actgctttgt ggcccgttgt ccgatcgctt
     8641 tggtcgcaag cgggtgattg agctgtgcgt ctttttgttt ggcgcgttaa gcctcgcctc
     8701 agctttctct ccggatctgc aaactctggt cttcctgcgc tttctcaccg gcttgggtct
     8761 gggcggcgcg atgccgaaca ccatcaccat gacctcggaa tatctgcctg cccgccgccg
     8821 cggcgcgctg gtgacgctga tgttttgcgg ctttaccctg ggttcggcgg ttggcgggat
     8881 tgtcagcgcg cagctggtgc cggtgattgg ctggcacggg atcctggtgt tgggtggcgt
     8941 attaccgctg atgctgtttg ttgcactgct ggtggttctg ccggaatcgc cacgctggca
     9001 agtgcgccgc cagctacccc aggcggtgat tgcgaaaacc gtcagcgcca tcacccgcga
     9061 gcgttatgtc gatacccagt tttatctcat tgaatcagcc tccgttacaa aaggcagcat
     9121 tcgtcaactg tttatggggc gtcagttacc catcacgttg atgctgtggg tggtgttctt
     9181 tatgagcttg ctgattattt acctgctctc aagctggatg ccaacgctgc tcaaccatcg
     9241 cggcatcgac ctgcaacatg cctcctgggt taccgccgcg ttccagattg gcggtacgtt
     9301 gggcgcgctg gcgctgggcg tgctgatgga taagttcaat cctttccgcg tactggcgct
     9361 gagctatgcc acaggcgcta tatgcatcgt gatgattggc ttaagccagg atggattatg
     9421 gctgatggcg ctggcgattt tcggcaccgg cattggcatc agcggatcgc aggtggggct
     9481 gaatgcgctg accgcaacac tttatccaac acaaagtcgc gccacgggcg tgagctggtc
     9541 aaacgccgtg ggccgctgcg gcgcgattgt tggctcgctg tctggcggcg tgatgatggc
     9601 gatgaatttc tctttcgaca ccctgttttt cattatcgct gtgcctgcgg cgatcagtgc
     9661 ggtgatgtta accctgctga tcaccgtggt tcgccagtcg acctctatcc ctgactcact
     9721 gcctcacgca ggtgttgtga acgaataagg agcatcccaa tgactgataa caatcaaaat
     9781 agccgtgaac aattttatca gcatatttcc gggcaaaacc tgaccccgct gtgggagtca
     9841 ctgcaccatc tggtgccgaa aacgcccaac gctaactgtg cgccagcgta ctggaattat
     9901 caggagatcc gcccgctgct gctggagagc ggcagcctga ttggcgcgaa agaggcggtg
     9961 cgccgcgtgc tggtactgga aaacccggcg ctgcgagggc agtcttccat caccgcgact
    10021 ttatatgccg gtttgcaact gatcatgccg ggcgaagtgg caccgagtca tcgtcataac
    10081 cagtcggcgc tgcgttttat tgttgaaggc aaaggggcat ttaccgccgt tgacggcgaa
    10141 cgcacgccaa tgaatgaggg cgattttatc ctgaccccgc agtggcgctg gcacgatcac
    10201 ggcaaccctg gcgacgaacc ggttatctgg ctcgatgggt tggatctgcc attagtgaac
    10261 actctgggct gcggttttgc cgaagattac ccggaagatc aacaaccggt aacgcgtaaa
    10321 gagggcgatt acctgccgcg ttacgctgcc aatatgttgc cgctgcgcca tcagacgggg
    10381 aactcctcgc cgatctttaa ctatcgttat gaccgcagcc gcgaagtgct gcacgattta
    10441 acccgcatgg gcgatgccga cgaatgggat ggctacaaaa tgcgctacgt caacccggtc
    10501 accggcggct atccgatgcc gtcgatgggc gctttcctgc aactgctacc gaaagggttc
    10561 tcctcccgcg tggcgcgcac cactgacagc actatctacc atgtagtgga aggtagcgga
    10621 caagtcacca tcggcaatga aatcttcacc tttagtgcaa aagatatctt tgtggtgccg
    10681 acctggcacg gcgtgtcatt ccagactacg caagataccg ttttattcag tttttcggac
    10741 agaccggtac aagaagccct ggggcttttc cgcgaagcgc gttattaatt tgggagagag
    10801 aaaatgactc aatatgtatt cgaaccacaa gcgcccgtta ccgtaccggt tgttggcagt
    10861 gataaacaat tccccgtgcg tcgcgtttac tgcgtcgggc gtaactatgc cgcccacgcc
    10921 cgcgaaatgg gctttgatcc ggatcgtgaa ccacccttct tcttctgcaa accagcagat
    10981 gccgttattc cggtcgccgc tggcgaaacg ctggcgctgc catatccggc gcagaccgat
    11041 aactatcatt acgaaattga actggtagtg gcgattggta aaaaaggcag cgatatcccg
    11101 ctggaacacg cccatgaata tgtctggggt tatgccacgg gtcttgatat gactcggcgt
    11161 gaccgtcaaa tggaaatgcg ccagatgggc cgcccgtggg aaatcggtaa agcgtttgat
    11221 ctctcagcgc ccatcgcgcc gctgcacaaa gcccgcgata tcggcggtat tgataatgcg
    11281 ccaatctggt tacaggttaa cggcgaagat catcagcgca gcgatattcg ccatctcatc
    11341 tggtcggtga atgaaaccat cagctatctg tcaggcttct ttgagctgca accgggcgat
    11401 ctgatcttca ccggtacgcc ggaaggggtt ggcccggtag tgaaaggtga tgtgatcaca
    11461 ggcaacgtcg agggcttaac gcctatcgcg gtgaagattg tctgaggtgc atgatgaagc
    11521 tgtacagttt ttttaatagt tcagcgtcgt accgggtacg cattgcgctg gcgctgaaag
    11581 gcattaatta ccagaccgaa ggggtgaata tccgtattgg tcagcaaaat gaactggcct
    11641 atcggcggat gaatccggtc gggctggtgc cgacgctgct gactgacgag ggccaatcac
    11701 tcggacaatc gctggcgatt attgactggc tggagaggca ctacccgcaa gttccgctgg
    11761 tgccgcagga agaaccggcc cgcaacaagg tgctggaaat tgtctatgcc atcgcttgtg
    11821 atattcatcc attaaataac ttgcgcgtgc tgcgctacct gaccgaagaa ctgaacgtca
    11881 gtgaagaaga aaaaaaacgt tggtatgcgc actggatcca gcaagggtta agcgcggtgg
    11941 aacaactgtt gcgccagaac cagtcggggc aattttgtgt gggcgagacg ccgacgctgg
    12001 cagattgctg ccttgtcccg cagtgggcaa acgcgctacg catgaactgc gatttgagcg
    12061 ggtatccacg ctgcaaagcg gtgtacgacg cctgcacaca gctgccagcg ttcattgccg
    12121 cagcgccaga aaatcaacaa gataaaattt cagcctgacc aggagaacta taatgacgaa
    12181 agtgacacgc gcagtaattg tgggaggcgg gatcggcggt gcggcaactg cattgtcact
    12241 ggcccgcctg gggatcaaag tggtgctgct ggaaaaggcg catgagatcg gtgagattgg
    12301 cgcgggcatc cagttggggc caaatgcgtt ttcggcgctg gacagcctcg gcgtcggcga
    12361 agttgcccgc cagcgcgcgg tgtttaccga tcacattacc atgatggatg cggtgaacgc
    12421 cgaagaagtg gtgcgcattg aaaccaggca ggcctttcgt gaccatttcg gcggtcctta
    12481 cgcggtgatc caccgggttg atattcacgc ctcggtgtgg gaagcggtac tgacgcatcc
    12541 ggacgtggaa tatcacacct ccaccaacgt cgtggatatt cgcgagacgg aagacgatgt
    12601 caccgtgttt gacgatcgcg gcaatagctg gaccggcgat attctgattg gctgtgacgg
    12661 cgtgaagtca gtggtgcgcc agagcttatt gggcgatgcg ccgcgtgtca ccggacatgt
    12721 tgtctaccgt gcggttatcg attgtgccga tatgccggaa gatttgcgca ttaacgcacc
    12781 ggtactgtgg gcggggccgc actgtcacct ggtgcattac cctttgcgcg gcggcacgca
    12841 gtataacctg gtggtgacat tccacagccg ccagcaggaa gagtggggcg tgaaggatgg
    12901 cagtaaagag gaagtgctct cttactttga ggggattcac ccgcgtccgc gccagatgct
    12961 ggataaaccg acttcatggc gacgctggtc aactgccgac cgtgaaccag tcgcgaaatg
    13021 gggcactaac cgcattacgc tggtgggcga tgcagcccat ccagtggcgc aatatatggc
    13081 gcaaggtgcc tgtatggcgc tggaagacgc ggtaacgctg ggtaaggcgc tggcgcagtg
    13141 tgagggtgat gcggcgcaag cctttgcgct gtatgagtcg gtacgtattc cgcgcaccgc
    13201 gcgtattgtc tggtcgaccc gtgagatggg acgggtttat cacgccgcgg gagtagaacg
    13261 ccaggttcgt aacctgctgt ggaaagggaa atcgcaggca gagttttatc gcggcatgga
    13321 gtggctgtac ggctggaaag aagataactg tctgcaacca cgctgagtac ttcttcacct
    13381 aaccaggcgc acgggtgatc tccttgccct tcggagagga gctattaagt cctgttgatt
    13441 tcaacagggc tctctcctct ccctttagcc tcctttaaaa taaggtatca tcagaaattc
    13501 ttctttcaac aaatagcacg ccacttcaag caaatcgtct ccagagctat tcagtgttac
    13561 gtcctgtttt tccagtgtaa atcatcacca gattatgtct gaaaagctcg ttacctacac
    13621 tgatcagtgt tagttgtttt ggcaaaaata tctcacaccg gttttcatca tcaacaagca
    13681 ctatacgcaa ccaacaacac aaaaaggaac acaccatgcc cccacacaaa atccgccagg
    13741 agtggctggc gctggacgag accgcagaag tcatcctaca gcacagcatc acgttgcaat
    13801 ccgccgacct tatccgccat tcttttattt ggcgcaaccg cagtatctgt ctgtgatgtt
    13861 ggtgttactg ccaagcgctt tatggttcgg gataacggca tgtatggtag caagtagctt
    13921 taagcgatgg ttataagttg ctcaaaaaat cggtcctcac tacaatacga attttatcag
    13981 gtatggaatt cggattatga ataaatctgt cataaagctg ttgaataaaa aattcattat
    14041 ttcagtaata acctcatcta catttattac tggatgcaca atggc
//
LOCUS       DAZFYC010000126        13844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFYC010000126 DAZFYC010000000
VERSION     DAZFYC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.126"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(6..317)
                     /locus_tag="ACHKW5_004151"
     CDS             complement(6..317)
                     /locus_tag="ACHKW5_004151"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7513423.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            516..1214
                     /locus_tag="ACHKW5_004152"
     CDS             516..1214
                     /locus_tag="ACHKW5_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7513424.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1259..2158)
                     /gene="eamA"
                     /locus_tag="ACHKW5_004153"
     CDS             complement(1259..2158)
                     /gene="eamA"
                     /locus_tag="ACHKW5_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO7513425.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGACTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2353..3540
                     /gene="ydeE"
                     /locus_tag="ACHKW5_004154"
     CDS             2353..3540
                     /gene="ydeE"
                     /locus_tag="ACHKW5_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO7513426.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSDAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3667..3762
                     /gene="mgtS"
                     /locus_tag="ACHKW5_004155"
     CDS             3667..3762
                     /gene="mgtS"
                     /locus_tag="ACHKW5_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO7513427.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3762..3866
                     /gene="mgtT"
                     /locus_tag="ACHKW5_004156"
     CDS             3762..3866
                     /gene="mgtT"
                     /locus_tag="ACHKW5_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO7513428.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3981..4871)
                     /gene="dgcZ"
                     /locus_tag="ACHKW5_004157"
     CDS             complement(3981..4871)
                     /gene="dgcZ"
                     /locus_tag="ACHKW5_004157"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO7513429.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTSALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5126..5518)
                     /gene="ydeI"
                     /locus_tag="ACHKW5_004158"
     CDS             complement(5126..5518)
                     /gene="ydeI"
                     /locus_tag="ACHKW5_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO7513430.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSMDKKSAPAVVRVTHLQK"
     gene            5794..6312
                     /gene="ydeJ"
                     /locus_tag="ACHKW5_004159"
     CDS             5794..6312
                     /gene="ydeJ"
                     /locus_tag="ACHKW5_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO7513431.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(6356..8401)
                     /gene="dcp"
                     /locus_tag="ACHKW5_004160"
     CDS             complement(6356..8401)
                     /gene="dcp"
                     /locus_tag="ACHKW5_004160"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO7513432.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHL
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQHRGLNI"
     gene            8538..9284
                     /gene="ydfG"
                     /locus_tag="ACHKW5_004161"
     CDS             8538..9284
                     /gene="ydfG"
                     /locus_tag="ACHKW5_004161"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO7513433.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            9373..10059
                     /gene="rspR"
                     /locus_tag="ACHKW5_004162"
                     /gene_synonym="ydfH"
     CDS             9373..10059
                     /gene="rspR"
                     /locus_tag="ACHKW5_004162"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO7513434.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            10237..10440
                     /gene="ydfZ"
                     /locus_tag="ACHKW5_004163"
     CDS             10237..10440
                     /gene="ydfZ"
                     /locus_tag="ACHKW5_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO7513435.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(10476..11936)
                     /gene="ydfI"
                     /locus_tag="ACHKW5_004164"
                     /pseudo
     CDS             complement(10476..11936)
                     /gene="ydfI"
                     /locus_tag="ACHKW5_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(12025..13308)
                     /gene="ydfJ"
                     /locus_tag="ACHKW5_004165"
                     /pseudo
     CDS             complement(12025..13308)
                     /gene="ydfJ"
                     /locus_tag="ACHKW5_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            13440..13682
                     /locus_tag="ACHKW5_004166"
     CDS             13440..13682
                     /locus_tag="ACHKW5_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7513436.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
BASE COUNT         3739 a         3409 c         3064 g         3632 t
ORIGIN      
        1 gattcttact cttgcagtag tttcatcgca aattcaagaa ctgcctgtcc gttcatggtg
       61 ccgtagtctt tcatctcgat aacggctaca ggcattttcc cctgtgcaac ggcttcaatc
      121 tctggtttct gaaagcgaac ttgcgggcca agtaaaacaa cgtcaacttc gttattttta
      181 attttccctt tagcctctgc aatcgccaat gcggaaatat taacttcaag tgaaatagcg
      241 ttagcatgat caatcatacg tttaaccagc atactggttg acatgcccgc agcgcaaact
      301 aacaatattt ttttcatatt ttcccttact ggtatataac agattacatt gtttaaatat
      361 catatcccgc agtagctata cgtcctggta cattgatcac agtaatacag tgataaagtt
      421 gttcaacatc agggtgatta ttaaacgact cggccatacg ataaaccaat cagaactcaa
      481 ctccttgttt tcaatggctc ttaaaaggtg caaaagtgat ttttcaaaag attgcccgct
      541 tacttaaatc cgaaatcaat ggcaattcat ggcatgttgg tgatttgttg ccgtcagaag
      601 cggaactcgc tgttcgttat aatgtttcgc gtaataccct gcgtaaggca ttatccttgc
      661 tggaaggcga aggaattatt cacagaaagc atggttcagg aacatacatt caaaaaaaga
      721 attttgttgc acacattgat cacatgaaca gtttcagtga aattgcgcat aaaagcggca
      781 aagaggcagg gagtcagatt atgaaatttg aagtgcagga tgcttcaccc actattgcga
      841 ctgagctgaa tttagtgact ggtgagcagg tttattacat aaagagactg cgattcattg
      901 aggataatgc agcacaatta gaagaaacgt ggatgtcagt agcacgtttt cctgatttaa
      961 ccgtgtcaca tatgcaaaaa tccaaatttt catatattga gaatgaatgc gggatcaaaa
     1021 tcattggcac ctttgaaact ttctccccta cttttcctac cccagaaatc gccagtattt
     1081 tacggatcag cccacgggat cccatactta aaattcagac ccaggctgtg gacagtaact
     1141 ctattccgct ggattattcg ttactttaca gcaatatttt cgagttccag gtaaagtact
     1201 ttttcccgcg ataaaagcag acgcacagag tgctgacatc acattctgtg cgtatggttt
     1261 agcctctaac ctttaccgct ttacgccacc gcaagccaaa tacattgata tacagcccgg
     1321 tcataatgag caccgcacct aaaaattgca gacccgttaa gcgttcatcc aacaatagtg
     1381 ccgcacttgc cagtccgact acgggcacca gtaacgataa cggtgcaacc cgccaggttt
     1441 catagcgtcc cagtaacgtc ccccagatcc cataaccaac aattgtcgcc acaaacgcca
     1501 gatacatcag cgacaagatg gtggtcatat caatagtaac cagactgtga atcatggatg
     1561 cggaaccatc gagaatcagc gaggcaacaa agaagggaat gattgggatt aaagcgctcc
     1621 agattaccag cgacatcacc gccggacgcg ttgagtgcga catgatcttt ttattgaaga
     1681 tgttgccaca cgcccaacta aatgctgcag ccagggtcaa cataaagccg agcatcgcca
     1741 catgctgacc gttcagacta tcttcgatta acaccagtac gccaaaaatc gctaaggcga
     1801 tccccgccaa ttgtttgcca tgcagtcgct ccccgaaagt acacgcgcca agcacgatag
     1861 taaaaaacgc ctgtgcctgt aacaccagcg aagccagtcc agcaggcata ccaaagttaa
     1921 tggcgcagaa cagaaaggca aactgcgcaa aactgatggt taatccatac cccaagagca
     1981 aattcagtgg tactttcggt cgtgcaacaa aaaagatggc cggaaaagcg accagcataa
     2041 agcgcaaacc ggccagcatc agcggtggca tgttatgaag ccccactttg atgaccacaa
     2101 aatttagccc ccatacgacc actaccagta gcgccaacac cccatctttt cgcgacattc
     2161 taccgcctct gaatttcatc ttttgtaagc aatcaactta gctgaattta cttttcttta
     2221 acagttgatt cgttagtcgc cggttacgac ggcattaatg cgcaaataag tcgctatact
     2281 tcggattttt gccatgctat ttctttacat ctctaaaaca aaacataacg aaacgcactg
     2341 ccggacagac aaatgaactt atccctacga cgctctacca gcgcccttct tgcctcgtcg
     2401 ttgttattaa ccatcggacg cggcgctacg ctgccattta tgaccattta cttgagtcgc
     2461 cagtacagcc tgagtgtcga tctaatcggt tatgcgatga caattgcgct cactattggc
     2521 gtcgttttta gcctcggttt tggtatcctg gcggataagt tcgacaagaa acgctatatg
     2581 ttactggcaa ttaccgcctt cgccagcggt tttattgcca ttcctttagt gaataacgtg
     2641 acgctggtgg tgctcttttt tgccctcatt aactgcgcct attctgtttt tgctaccgtg
     2701 ctgaaagcct ggtttgccga caatctttcg tccaccagca aaacgaaaat cttctcaatc
     2761 aactacacca tgctcaacat tggctggacc atcggtccgc cgctcggcac gctgttggtg
     2821 atgcagagca tcaatctgcc cttttggctg gcagctatct gttccgcgtt tcccatgctt
     2881 ttcattcaaa tttgggtaaa gcgcagcgag aaaatcatcg ccacggaaac aggcagtgtc
     2941 tggtcgccga aagttttatt acaagataaa gcactgttgt ggtttacctg ctctggtttt
     3001 ctggcttctt ttgtaagcga cgcatttgct tcatgcattt cacaatatgt gatggtgatt
     3061 gctgatgggg attttgccga aaaggtggtc gcggttgttc ttccggtgaa tgctgccatg
     3121 gtggttacgt tgcaatattc cgtgggccgc cgacttaacc cggctaacat ccgcgcgctg
     3181 atgacagcag gcaccctctg tttcgtcatc ggtctggtcg gttttatttt ttccggcaac
     3241 agcctgctat tgtggggtat gtcagctgcg gtatttactg tcggtgaaat catttatgcg
     3301 ccgggcgagt atatgttgat tgaccatatt gcgccgccag gaatgaaagc cagctatttt
     3361 tccgcccagt ctttaggctg gcttggtgcc gcgattaacc cattagtgag tggcgtagtg
     3421 ctaaccagcc tgccgccttc ctcgctgttt gtcatcttag cgttggtgat cattgctgcg
     3481 tgggtgctga tgttaaaagg gattcgagca agaccgtggg ggcagcccgc gctttgttga
     3541 tttaagtcga acacaataaa gatttaattc agccttcgtt taggttacct ctgctaatat
     3601 ctttctcatt gagatgaaaa ttaaggtaag cgaggaaaca caccacacca taaacggagg
     3661 caaataatgc tgggtaatat gaatgttttt atggccgtac tgggaataat tttattttct
     3721 ggttttctgg ccgcgtattt cagccacaaa tgggatgact aatgaacgga gataatccct
     3781 cacctaaccg gccccttgtt acagttgtgt acaaggggcc tgatttttat gacggcgaaa
     3841 aaaaaccgcc agtaaaccgg cggtgaatgc ttgcatggat agatttgtgt tttgctttta
     3901 cgctaacagg cattttcctg cactgataac ggatcgttga cacagtagca tcagttttct
     3961 caatgaatgt taaacggagc ttaaactcgg ttaatcacgt tttgttcgtc aataaacatg
     4021 cagcgatttc ttccggtttg cttaccctca tacattgccc ggtccgctct tccaataacc
     4081 acatccagag gctcttcagg aaatgcgcga ctcacacctg ctgtcacggt aatgttgata
     4141 tgcccttcag aatgtgtgat ggcatggtta tcgactaact ggcaaattct gacacctgca
     4201 cgacatgctt cttcatcatt agccgctttg acaataatga taaattcttc gcccccgtag
     4261 cgataaaccg tttcgtaatc acgcgtccaa ctggctaagt aagttgccag ggtgcgtaat
     4321 actacatcgc cgattaaatg cccgtaggta tcattaacca atttaaatcg gtcaatatcc
     4381 aacaacatta aataaagatt cagaggctca gcgttgcgta actgatgatc aaaggattca
     4441 tcaagaaccc gacgacccgg caatcccgtc aaaacatcca tattgctacg gatcgtcagc
     4501 aaataaattt tgtaatcggt taatgccgaa gtaaaagaaa gcaacccctc ctgaaaggcg
     4561 tcgaaatgcg cgtcctgcca gtgattttca acaatagcca gcattaattc ccgaccacag
     4621 ttatgcatat gttgatgggc agaatccatt agccgaacgt aaggtaattc atcgttatcg
     4681 agtggcccca gatgatcaat ccaccgacca aactggcaca gtccataaga atggttatcc
     4741 gttatttctg gcttactggc atctctcgcg accacgctgt gaaacatact caccagccac
     4801 tggtagtggg catcgatagc cttattgaga tttaacaaga tggcatcaat ttccgttgtc
     4861 ttcttgatca ttgccactcc tttttcacag ttccttgtgc gcgctattct aacgagagaa
     4921 aagcaaaatt acgtcaatat tttcatagaa atccgaagtt atgagtcatc tctgagataa
     4981 cattgtgatt taaaacaaaa tcagcggata aaaaagtgtt taattctgta aatcacctct
     5041 gcattatcgt aaataaaagg atgacaaata gcataaccca ataccctaat ggcccagtag
     5101 ttcaggccat caggctaatt tatttttatt tctgcaaatg agtgacccga acgacggccg
     5161 gcgcgctttt cttatccata ctgccgctaa tgttgatcat ctggtccggc tgaacttctc
     5221 gtccatcaaa gacggccgca ggaataacga cattaatttc accgctctta tcgcgaaaaa
     5281 cgtaacggtc ctctcctttg tgagaaatca aattaccgcg tagtgaaacc gaagcgccat
     5341 cgtgcatgtt ttttgcgaaa tcaacggtca ttttttttgc atcatcggtt ccgcgatagc
     5401 catcttctat tgcatgaggc ggcggtggcg ctgcatcctg ttttaaaccg ccctggtcat
     5461 ctgccaacgc ataaggcatg acaagaaaac ttgctaatac aatggcctga aatttcatac
     5521 tgactcctta attgcgtttg gtttgactta ttaagtctgg ttgctatttt tataattgcc
     5581 aaataagaat attgccaatt gttataaggc atttaaaatc agccaactag ctgtcaaata
     5641 tacagagaat ttaactcact aaagttaaga agattgaaaa gtcttaaaca tattttcaga
     5701 ataatcggat ttatatattt gaaaattatt atattggacg agcatacaga aaaagcaaat
     5761 cacctttaca tataaaagcg tggacaaaaa acagtgaaca ttaatagaga taaaattgta
     5821 caacttgtag ataccgatac tattgaaaac ctgacatccg cgttgagtca aagacttatc
     5881 gcggatcaat tacgcttaac taccgccgaa tcatgcaccg gcggtaagtt ggctagcgcc
     5941 ctgtgtgcag ctgaagatac acccaaattt tacggtgcag gctttgttac tttcaccgat
     6001 caggcaaaga tgaaaatcct cagcgtaagc cagcaatctc ttgaacgata ttctgcggtg
     6061 agtgagaaag tggcagcaga aatggcaacc ggtgccatag agcgtgcgga tgctgatgtc
     6121 agtattgcca ttaccggcta cggcggaccg gagggcggtg aagatggtac gccagcgggt
     6181 accgtctggt ttgcgtggca tattaaaggc cagaactaca ctgcggttat gcattttgct
     6241 ggcgactgcg aaacggtatt agctttagcg gtgaggtttg ccctcgccca gctgctgcaa
     6301 ttactgctat aaccaggctg gcctggcgat atctcaggcc agccattggt ggtgtttata
     6361 tgttcaagcc acgatgttgc agcatcggca taatcttagg tgccttaccg cgccattgtc
     6421 gatacaggcg ttccagatct tcgctgttac ctctggaaag gatcgcctcg cgaaaacgct
     6481 gcccattttc acgcgttaat ccgccctgct caacaaacca ctggtaacca tcatcggcca
     6541 acatttgcgt ccacagataa gcgtaataac ctgcagcata tccgccacca aaaatatggg
     6601 cgaaataact gctgcgatag cgtggcggta tagcaggaag atccatattt tccgccacca
     6661 gcgcccgcaa ttcaaaatca tcgacatcct gcattgcttc gttttcttcc aggcaatgcc
     6721 agcgcatatc gagaagtgcg gcgctaagca gttcgctcat ctcataccct ttgttgaaca
     6781 ggctggcatt acgcattttc tgttgcagtt cgtcaggcat tgctgccccg ctctgataat
     6841 gccgggcgta gcgagcgaat acctgcggat gcgttgccaa gtgttcgttg atttgcgacg
     6901 gaaattcgac aaaatcacgc ggcgtgttgg tgccggaaag cgtggcataa cgctggcggg
     6961 caaaaaggcc gtgcagcgta tgaccaaatt catggaataa ggttattaca tcatcccaga
     7021 gtaacaacgc aggctcaccg gcagcgggtt tctgataatt gcaaacgtta taaattaccg
     7081 gatgcgtttc attaagcgtt gattgctcaa caaaattgcc catccatgca ccgccgcttt
     7141 ttgaatcacg ggcgaagaaa tcaccgtaaa ataacgccag tcccacgcca ttatgatcaa
     7201 aaatttccca cacacgaacg tcaggatggt agacaggaat atcaaaacgt tcgacaaact
     7261 taataccgaa gagctgattc gcggtccaga atacaccttc attcaacacc gtgtttaatt
     7321 caaaatatgg cttgagctgc gcctcatcaa gatcaaattt ctcccgccgt acctgttcgg
     7381 cataaaatgc ccagtcccac ggctgcgcgc taaacccgcc ttgctgctta tcgataaccg
     7441 cctgtatgga ggctaattca tcgctagcac gttgacgcgc cgctggaaca atttcccgca
     7501 taaagttgag tgctgcttca ggtgtttttg ccatctgatc ggcgattttc catgcggcat
     7561 aatgaggaaa accaagcagt ttcgcctgct gtgcgcgaat ctctaccaga cgttgaatga
     7621 tagcgcgggt atcattgcca tcattttttt ccgctcgcgt ccagcccgca ataaacagtt
     7681 tttcacgcgt cgcacgatcg cgcatttcgg caagcgccgg ttgttgggtg gtattcagca
     7741 gcggaatcag ccatttgtta tccagacctt tctcgcgagc cgccactgcc gccagcgcaa
     7801 tctcttgctc actcattcct gccagctgcg cgatatcgtt cacaaccaga ccgccggatt
     7861 tatttgctgc aagtaaccgc tggttaaact ggctggtcag ggtcgcagct tctgtattca
     7921 gtacttttaa ttttgcttta tcagcttgcg caagtttggc tccggcaagg acaaaacgtt
     7981 gatgaataac ctccaccagg cggatggatt cactatcaag ccccagggat tcacggcgct
     8041 gccagacagc atctacccgc gcgaataatt caccgttcag atagatatca ttagccagtt
     8101 ccgccagttc agcggaaaac tgttcatcaa gacgctgtaa ttcatcattg gtatgcgccg
     8161 cagtcatcgc aaaaaagacg ctggtaacgc gggtaagtaa ttctccgctt tgttccagtg
     8221 ccagaatagt attgttgaaa tcaggcgttt gcgggttaag cgcgatggca gcaatttctg
     8281 cccgcttttg ctgcattccc tcatcgaatg ccgggcgata gtgatgattg gcaatttgat
     8341 caaaatgagg agccagatac ggcagtgtgc tttgcacaag gaaaggattc attgttgtca
     8401 ttttcttctc ctgaacgcga ggtgttccat agcgtaggct tactgataac gtagtgcaat
     8461 cttgcaaacc agtattaccc gctcttaagc atcacgtgct atgttattga cacacaaaag
     8521 cgttgaggaa cagtgagatg atcgttttag taactggagc aacggcaggt tttggtgaat
     8581 gcattactcg tcgttttatt caacaagggc ataaagttat cgccactggc cgtcgccagg
     8641 agcggttgca ggagttaaaa gacgaactgg gagataatct gtatatcgcc caactggacg
     8701 ttcgcaaccg cgccgctatt gaagagatgc tggcatcgct tcctgccgag tggtgcaata
     8761 ttgatatcct ggtaaataat gccggcttgg cgttgggcat ggagcctgcg cataaagcca
     8821 gcgttgaaga ctgggaaacg atgattgata ccaacaacaa aggcctggta tatatgacgc
     8881 gcgccgtctt accgggtatg gttgaacgta atcatggtca tattattaac attggctcaa
     8941 cggcaggtag ctggccgtat gccggtggta acgtttacgg tgcgacgaaa gcgtttgttc
     9001 gtcagtttag cctgaatctg cgtacggatc tgcatggtac ggcggtgcgc gtcaccgaca
     9061 tcgaaccggg tctggtgggt ggcaccgagt tttccaatgt ccgctttaaa ggcgatgacg
     9121 gtaaagcgga aaaaacctat caaaataccg ttgcattgac gccagaagat gtcagcgaag
     9181 ccgtctggtg ggtgtcaacg ctgcctgctc acgtcaatat caataccctg gaaatgatgc
     9241 cggttaccca aagctatgcc ggactgaatg tccaccgtca gtaattttta tacccggcgt
     9301 aactgccggg ttattgcttg tcacaaaaaa gtggtagact catgcagtta actcactcac
     9361 aagcaagaac gaatgaccgt cgaaacgcaa cttaatccca cacagcctgt caatcagcag
     9421 atttatcgta ttcttcgtcg cgacattgtc cattgcctga ttgcaccagg cacaccgttg
     9481 tcggaaaaag aagtttctgt tcgtttcaat gtgtcacgcc agccggttcg tgaagccttt
     9541 attaaactgg cggaaaacgg cctgattcaa attcgtccgc agcgtggcag ctacgtcaac
     9601 aaaatttcca tggcccaggt gcgaaacggc agttttatcc gtcaggccat tgagtgcgcg
     9661 gtggcgcgtc gggcggcgag catgattacc gaaagccagt gctaccaact ggaacaaaat
     9721 cttcaccagc aacgcattgc cattgagcgc aagcaactgg atgatttttt tgaacttgat
     9781 gacaacttcc atcaactctt gacgcagatt gccgactgtc aactggcgtg ggataccatt
     9841 gagaacctga aagcgaccgt tgatcgcgtg cgctatatga gtttcgacca tgtttctcca
     9901 ccagaaatgc tgttacgcca gcatcttgat attttctctg ccctgcaaaa acgtgatggc
     9961 gatgcggtag aacgtgcaat gacgcaacat ttgcaggaaa tcagcgaatc cgtgcgccag
    10021 atccgccagg aaaacagcga ctggtttagc gaagagtaat tcatttactc tcatcccatc
    10081 cgggatgaga ggcttttccc cccgccttac ggctcatgca tgcatcaaaa aagatgtgag
    10141 cttgatcaaa aacaaaaaat atttcactcg acaggagtat ttatattgcg cccgttacgt
    10201 gggcttcgac tgtaaatcag aaaggagaaa acacctatga cgacctacga tcgtaaccgt
    10261 aacgcaatca ccactggcag ccgtgttatg gttagcggca ccggtcacac tggcaagatc
    10321 ctgtcgattg atactgaagg tctgaccgct gagcaaatcc gccgcggaaa aaccgtagtt
    10381 gttgaaggtt gtgaagagaa actggcacct ctggacctga ttcgtctcgg catgaactaa
    10441 gtgtgtgaat gccgccgatg gcggcattgc tttttttact tcacggaata tttcgccacg
    10501 gtcgctttcg cgccatgcgc taataaagac aagtacgctt ccgtcacttt tgcagtaaac
    10561 aagctatcgt ctggcaaatc atcaccaaag attgccttaa tcgccagcaa tgactggacg
    10621 cgcgctttcc cttcggcact actttgtaca gccttctgaa taacaggtaa cagtgggtca
    10681 ctgatttcta tcggatttcc ctgttcatca acaccaccga cataacgcat ccaacccgcg
    10741 acgcccagcg ccagcagatc gaacttgctg tcatgcgcca gatgccagcg aacagaatcc
    10801 aacatccgct gtggcaattt ctggctgcca tccatcgcaa tctgccaggt tcgatgacgt
    10861 aacgccgggt tgctatagcg tgcaattaat cggttagcgt aatcctgcaa atcaacgccc
    10921 tgcactttca gcgtcggcgc ttgttcctgc aacatcaagc catacgccgc atgacgataa
    10981 tgttcatctt ccatgcagtc attaatgtgc tgatatccgg caagataccc cagatacgcc
    11041 aggaatgaat gactgccgtt gagcatgcgc aacttcatct cttcataagg cagcacatcg
    11101 ctaaccagtt cggctcccgc tttttcccat tccggacgtc cggcaacaaa gttatcttct
    11161 attacccact ggcggaaagg ttcacaggca acgcccgcag gatcgcgcac accggtaagt
    11221 tgttcgattt tcgccagcgt atcctctgtc actgcgggca caatacggtc caccattgtt
    11281 gatgggaaag tcacgttatc ttcgatccat tgtgccagtt ttacatcaac ggcttgcgcg
    11341 taggaagtga caacatcacg cataacatga ccgttttctg gcatgttgtc acatgacatg
    11401 acggtaaatg cgggaagtcc tgccgcttta cggcgagcca gcgcctcaac aatcacccct
    11461 gttgctgttt tcggctggtg gggattttgc acgtcggcag cgaccatcgg gtgatcgagc
    11521 attaactgtc cggtcgccgg agagtggaaa tacccttttt cggtgattgt cagagagaca
    11581 atcgcgattt gcggttcaca catcgcagcc aacacggttt ctaagccatc catctgtacg
    11641 tgcaaggctt ttttaacgac gccaacgacg cgagccgtcc acgcatcggc cgacatttcc
    11701 gcaacggtat aaagattatc ttgctgttgt aaatcggcaa tttgctgttc gccgccgatt
    11761 aagttgacct aataatatcc ccagtcactg aaatgttctg tagcaagaat atcggcatac
    11821 acgccctgat gcgcacggtg aaatgcacca aagcctaaat gaacaattct tggggccagg
    11881 ttattacgat cataaacagg gagtgtcgct tttgctgata acaaattatt tcccataaca
    11941 attccttaaa tttaaatata gcaagctaca tgttttgtta tatgaataaa aatcccctct
    12001 ccggtaagag aagggattga ggatttacag acttctggaa ggttgcgcag ctcttacaac
    12061 acgcggttga tcttccgcag cgtcttccag cgcacttaaa tcacggtctt tcacctctgg
    12121 cattttcagc gcagagatta aaccaatcac tgaatatgcc atgatcataa tggcgatcgg
    12181 ataccaggat tccgtcatgg tgcagaaaat acccgccagg ataggaccaa aaccggaagc
    12241 gataagacca ccaatttctt tagaaatagc catccgggta aagcggtttt tacagccgaa
    12301 catttctgcc atggtaatgt tttccagagc aaataatccc agcaccgcac agttatgaat
    12361 cacaatcagt gcaaccataa tggtgctcgg ggcatagctt ttatctacaa tgatagaaag
    12421 cattggccat gccagcacaa tcgcggaggt attcataata atatacggga tccggcgacc
    12481 aattttatcg gataaccaac caaggaacgg aatggtcata aagccgagaa tcgaactaat
    12541 catcaatgca tctgttggaa ttgctttgtt aaacaataac gtctgcacta aatagcctgc
    12601 aaggaaagtc tgaattaaac ctgagttacc agcctgacca aaacgcagcc ctgttgccag
    12661 ccagaaggat ttgctctgga acatgctacc agcaggtgca ggttttgctg tcggttggtt
    12721 gctgtcatta accttctcaa agaccgggct ttctttcaga ttcatacgca accagatagc
    12781 aaagaccatc acgacaacgc tcgccaggaa cggtatacgc catccccacg ccagcagttc
    12841 ctctttactg agaatgaaga acataaaggc ccagattgcc gttgcgctca aggttccgca
    12901 gttagttccc atagctacaa atgaggagat aattccgcgc ttaccttttg gcgcatattc
    12961 cgccagcatc gtaccggcac cggaaatttc cgcacctgca cccaacccct gaataatacg
    13021 cagcgtcacc agcaagatgg gtgcaaaaac accaatctgt gcataggtcg gtaacacacc
    13081 aattaaggtg gtacagatcc ccatcatggt gatggtaata aagagcactt ttttacgccc
    13141 tattctgtcg cccattttgc cgaaaataaa tgctccgaca atacgcgcca cataacctgc
    13201 accgtaggtt cccattgcca gaattaacgc cattgccgtt gatgattcag gaaaaaatat
    13261 ttcatgaaac actaacgctg cgccgagcga atataactgg aaatccataa ttcatagatg
    13321 ttttttacta ttctgtgggt ttttgggtgt tttctaagtt ttttcagatg gttgtatttt
    13381 ttctaaaaat ccctaatctc gattttgctg tttatttgag gcctttttat gtcccatata
    13441 tgccccacag ataccccgca gccaaaatca acaaaatgcc aaaaggttct gttcctgccc
    13501 tgcaacaaga aatgctgcga cgtgtcagta aacgttatga cgatgtagaa gtgatcatca
    13561 aatccaccag caacgatggc ctttcagtta ctcgcaccgc cgataaagat tctgcaaaaa
    13621 cttttgttca ggagacgctg aaagatacct gggagtctgc tgacgagtgg tttgttcgct
    13681 aattaacgag taaaatcagt aacggctgga aatcattcaa tactcacact atctaaaagt
    13741 tcaccagcca acagcggtaa gttctttcat acgacgtgcc gcggatccta ttattctagt
    13801 atgaaatccc taaccgattt caaattctcc agactgctca gatt
//
LOCUS       DAZFYC010000127        13831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFYC010000127 DAZFYC010000000
VERSION     DAZFYC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.127"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..6212
                     /gene="efa1"
                     /locus_tag="ACHKW5_004167"
     CDS             <1..6212
                     /gene="efa1"
                     /locus_tag="ACHKW5_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO7513437.1"
                     /translation="FKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITGVHTEENLLPA
                     PSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELPPHELLTPV
                     LGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMREGTL
                     NSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADATLSL
                     LSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVASSLHF
                     IAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALLYL
                     QTKDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKYI
                     TNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFGHADF
                     LSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPENDAGLS
                     TRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSLRLNG
                     DVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQRQ
                     ATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGETLHTT
                     SVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTLAKIN
                     LAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTMGKAL
                     SASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLTAASV
                     AFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVASPERG
                     LLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYANSIS
                     PYSSLAHGYANSKWPRTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWREAVPD
                     STSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFILRGG
                     SGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLTFDLSKQTQSVMLKTPDDEV
                     PVMTITQKGINTLVGTAAGKDRLIGNDKDNTFHTSSGGGTVISGGGNNRYIIPRDLKT
                     PLTLTLSSNSVSHEIFLPETTLAELKPVAFELSLIYWAGNNINVQPEDEAKLNHFAGN
                     FRVHTRDGMTLEAVSRENGIQLAISLCDVQRWQAVYPEENNRPDAILDRLHDMGWSLT
                     PEVRFQGGETQVSYDPLTRQLVYQLQARYSEFQLAGSRHHTTAVTGTPGSRYIIMKPV
                     TTQILPTQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLTISGI
                     EESLPETTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMALNQVM
                     TFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDLSIPP
                     YSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEAPSLI
                     LSEFRRFTISSDKTFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFRDFFT
                     EVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYARIQK
                     EETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGELFPTFHPWYTDDLSGRYKS
                     VPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILSEIPQ
                     NNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHSSGRV
                     YSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRSTVSK
                     WSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIADNAP
                     GETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6384..6734
                     /locus_tag="ACHKW5_004168"
                     /pseudo
     CDS             6384..6734
                     /locus_tag="ACHKW5_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6866..7291
                     /locus_tag="ACHKW5_004169"
     CDS             6866..7291
                     /locus_tag="ACHKW5_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7513438.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGHVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTMLIRELSGRGR"
     gene            7288..7638
                     /gene="tnpB"
                     /locus_tag="ACHKW5_004170"
     CDS             7288..7638
                     /gene="tnpB"
                     /locus_tag="ACHKW5_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7513439.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            7669..9254
                     /locus_tag="ACHKW5_004171"
                     /pseudo
     CDS             7669..9254
                     /locus_tag="ACHKW5_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            9283..9426
                     /locus_tag="ACHKW5_004172"
                     /pseudo
     CDS             9283..9426
                     /locus_tag="ACHKW5_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(9679..9834)
                     /locus_tag="ACHKW5_004173"
                     /pseudo
     CDS             complement(9679..9834)
                     /locus_tag="ACHKW5_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(10121..10546)
                     /locus_tag="ACHKW5_004174"
     CDS             complement(10121..10546)
                     /locus_tag="ACHKW5_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7513440.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(10543..10926)
                     /locus_tag="ACHKW5_004175"
     CDS             complement(10543..10926)
                     /locus_tag="ACHKW5_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7513441.1"
                     /translation="MYAKSFLALDGNGRLTGARTAHTAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERKEIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            11052..11706
                     /locus_tag="ACHKW5_004176"
                     /pseudo
     CDS             11052..11706
                     /locus_tag="ACHKW5_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12379..>13831
                     /gene="ltrA"
                     /locus_tag="ACHKW5_004177"
     CDS             12379..>13831
                     /gene="ltrA"
                     /locus_tag="ACHKW5_004177"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO7513442.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRLIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTKH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTGT"
BASE COUNT         3832 a         3316 c         3483 g         3200 t
ORIGIN      
        1 tatttaaagg gcaaacactg gatcgtcttt atcatgaagg atatgtattt tctgatatca
       61 ataccttgtc tcgttataca ctacacggac taggaataac tggtgtacat actgaagaaa
      121 acctgctacc agctccttca tcgtccttaa ttaatatatt gaaagaacat tataatgaag
      181 atgaaattag tgcgaaatta ccactagcat atgattacat tttaaataaa aaagaatcaa
      241 gctctattcc tgttgaaatt ttgaacaaac tttcagagtt accaccacat gaactactca
      301 cacctgttct tggccagagt gttaatcctc tgggcatggg ctactcatcc gataatggaa
      361 aaatcacaga gcaagtaata gtcagtggag ctgatggatt tgataatccc atatctggac
      421 ttatatatac ctatcttgaa gatctatata acatccatgt aaggatgcga gaaggtacac
      481 taaattcaca gaatcttcgt cagcttctgg aaaactctgt ttcttcatgc tttttgactg
      541 agcaaagtat taataaatta cttagtgagg cagaaaaaag gccttatcag tctttaacag
      601 aaatacatca gcatctcaca ggattaccaa ctattgccga tgcaaccctt tcattacttt
      661 ctgttggatt acctggtacg ggaaaactat tgcgcaggga gcaggactat gggcgtccac
      721 cagttacagc aattcaggat tccacatttg tactccctta taatttcaaa ggtattggtt
      781 ttaacgataa cattatatcc tctgcacctg tagcctcctc attacatttt atcgctgaac
      841 atgcgaaata tactttattg tcatggcctg agttttatcg tcatcatgca cagcgatggt
      901 tcgaaatggc taaaggatat ggaagccaga atattgattt tcaccctcag tctctattgg
      961 taacccaaga aggacgctgt atgggattag ccttacttta tttacagact aaagatactg
     1021 ctcattatag cattctccag gaaaacctaa tgactgtgag tgcacttcat cagaccagta
     1081 atcgcgataa gttgccactg tccaaagatg ataattcctt aatgacaaga acttatagtc
     1141 tgattgaaat gctacagtat cagggaaaca aatatattac caacgaatcg ctactacata
     1201 agaccgcatg gaaccaagaa agaataactt tattattcaa tgaaaaagga gttaagcgag
     1261 ccctgataag cacgcctaat catactctgg ttctgcaaca actggaggat atttaccggc
     1321 tcactgatcc aaattttggg catgcagatt tcctttcacc tatagatgct ctgaagttta
     1381 ttgaggctat gatacaatta actccaacac ttcaggaata ttatggccta ttaaacaaag
     1441 acattaataa acatatacaa gtacattatg ccgaatcaga tatggtctgg aataagcttc
     1501 tgccagaaaa tgatgctgga ctgagcacca gaattcagca caccaccacc gaccgtctgg
     1561 cgaatctggc tgaaccagtc gctgttgcag gtatctccct gccagtaaaa acactttatg
     1621 atatcggagc cacccttgac ggtcggcgca tcacctctcc tccaacatcg gagcaaatcc
     1681 cttctctgcg tctcaacggt gatgttctga atgattatct gtcccgcaca gttctgactc
     1741 cagaacaggc tgataacata agaaaaatac tgcacactca gggaatacgc agcggtaccc
     1801 gtcccataga tccggagatg attcgtggga cgcaggatga cctagtttcg tcacagactc
     1861 gtctgcaaag gcaagcaaca cgggttaaac agcaactcgc cggtgtactt gatactctgc
     1921 aacagcactt ccagaacatt ccacgttcat ccggtcgtca tctttctgta gagaatattg
     1981 agctggctga tatcggaagt gggcgtttca accttcaaat tcgagatgga gaaacattgc
     2041 ataccacttc tgtggaagta ccggaagttg tgtcccgttt tcagaaactt tctaccatgc
     2101 tttcagccct gcctgccagt ggaatcatgg atttcgacct cggcatgagt gtggtcggag
     2161 tcgtccagta tgcccgcctg ctacagcagg ggcacgaaga cagtacccta gccaagataa
     2221 atctagctat ggatatcaag caactttccg aagcaactct cggcagcatg attcagattg
     2281 ccgggaataa gtttctcaat acagaaggaa tccaggggtt cagactggaa agcgccgtcg
     2341 ctgaaggtat gcgctcagta gcaacccgta ccggaggcac aatggggaaa gccctttctg
     2401 ccagtgcccg tgttcttgaa ctgcctgtac tggaaacagt tctggggaca tggaacctgt
     2461 acaacagcgt cattcagctc cagcaagcta catcttattc tgagacaatg gctgcccggg
     2521 tacagattgc gtttgattcc atttctctgg gattaactgc cgcttcggta gctttcccgc
     2581 cactaattat tgccactggc cccattgcgg ctattggtat gggagcttcc agtattgcac
     2641 gtaatgtggc acggaaagaa gaacggcata cacaatggct ggaatataaa aaattcctga
     2701 ctgatggcag taaacacatt gttgtggcct ctccggaaag aggtctgctg gatttctccg
     2761 gaaacaaagt ttttggaaaa atggtgctgg atctgcgtca gtctcctcct ctcttgcatg
     2821 gagaaagctc ttttaacgct gaccgcaaaa tcggtcatcg tccggatctg ggggactggc
     2881 aaattcgtga gaaggtgggg tatgccaaca gtatcagtcc ctactcttct ctggcgcacg
     2941 gttacgccaa cagtaaatgg ccacgaacaa taccgaaaat tccctcggga gaatatgaca
     3001 ccataattct gggctacggt caccagtatc aggccaatac ggaaatagaa tatctgtcta
     3061 actggattgt atggcgggaa gccgtaccag acagtacttc ccgccacaaa cgtcctcctc
     3121 tggaggttct taatagtcag tgtactgtga tagctggaga gcgtaaaacc acagtacttc
     3181 ccctgagagt gctcagcgat ctgacaccgg aatgcacaga acaggctata tcgttaaaag
     3241 attataaatt catactgaga gggggaagcg gtgggctggc tgttcaggtc ggtggcgcgg
     3301 gatattatga tattgatgca aatcctgtgg caaaagaaaa tacgctctct tttcgcgggc
     3361 taccggaaga gtttccgctc acctttgatt tatcaaaaca aacacagtcg gtcatgctga
     3421 aaacaccaga cgatgaggtg ccggtaatga ccattaccca gaagggaata aacaccctgg
     3481 taggtacagc cgccggtaaa gaccgactaa tcggtaacga taaggacaat accttccata
     3541 caagctctgg cggcggtaca gtcatctccg gaggcgggaa taaccgctat attatccccc
     3601 gggatttaaa aacgccgttg acactgacgc tgtccagtaa ctcagtctct cacgaaatct
     3661 ttctgccaga aacaacccta gctgaattaa aacctgtcgc ctttgagctg agtttgattt
     3721 actgggccgg gaacaacata aatgttcaac cagaggatga agcaaaactg aaccactttg
     3781 ccggaaactt cagggtgcat acccgtgatg gcatgactct ggaggcggtt tcccgggaaa
     3841 atggtattca actggcgatt tcattatgtg atgttcaacg ctggcaggct gtttatccgg
     3901 aagaaaataa cagaccggat gccatactgg acaggctgca tgatatgggc tggagtctga
     3961 caccagaagt ccggttccaa ggaggagaaa cacaagtcag ctatgatccc ctgactcgtc
     4021 agctcgttta ccagcttcag gcgcgttact ctgaattcca gttggccggt agtcgccacc
     4081 ataccacggc tgtaaccgga actccgggaa gccgatacat tatcatgaag ccagttacaa
     4141 cacagatatt accgacacaa atcatactgg ctggtgataa tgaccatccg gaaacgattg
     4201 atttactgga agctagtcct gttctggttg aagggaaaaa agacaaaaac agcgtgatat
     4261 taacgattgc tacgattcag tattcccttc aactgacaat atccgggatc gaagaatcgc
     4321 tgcccgagac aacccgtgtg gcaattcagc ctcaggatac ccgtttactg ggtgacgtac
     4381 tccggatctt accagataat ggtaactggg tggggatttt ccggagtggt catacaccaa
     4441 cggtaaaccg gctggaaaat ttgatggcac tgaatcaggt aatgacgttc ctgccccggg
     4501 tatccggaag tgcagagcag gtattatgcc tcgaaaacct aggtggcgta aggaaaaaag
     4561 tggaggggga gttactgtca gggaagctga aaggtgcgtg gaaagccgaa ggtgaaccta
     4621 ctgttccggt aaatatctca gatctaagta tcccacccta ttcacgtctg tatctgattt
     4681 ttgaagggaa aaataatgtg ttgctacgca gtaaagtaca tgcagctccg ttgaaaataa
     4741 catccgcagg agagatgcag ttatctgaaa ggcagtggca acagcaggaa catattattg
     4801 tcaagcccga caacgaagcc ccctcattaa tactcagtga atttcgtcgt ttcactattt
     4861 catcggataa aacattttct ttaaaactga tgtgccatca gggtatggtc cgcatcgacc
     4921 gcagatcatt atcggtcaga ttgttctatc tgcgtgaaca gccaggtatc ggcagtttac
     4981 gtctgacgtt cagagatttt ttcacagaag tgatggatac aacggacagg gaaattctgg
     5041 agaaagagct gagaccaatt ctgataggag atacacaccg ctttatcaac gctgcataca
     5101 aaaatcatct gaatatccag ttaggagatg gcgttctgaa tctggcagac attgttgcgg
     5161 aatacgcccg tattcaaaag gaagaaacat caaaaatatt gtatcaatat caaggtgcca
     5221 tgaaaaaaaa aacagatgga ccatctgtgg tagaagatgc cattatgacc actactgtca
     5281 caacagattc aggtgaacta ttccctacct tccacccgtg gtatacagat gatttatcag
     5341 ggcgttataa gagcgtacct atggcaagaa aagcagatac tttgtatcac ctgacaccaa
     5401 aaggtgatct acagataata tatcaggtag ctacaaaaat ggtgaatcag gcgatgattg
     5461 tatccctgcc aaactaccga cacgagtggg aaaaatataa tttaagcatc ttatccgaaa
     5521 tccctcagaa caataatact gttgtacatt caatcctcag ggttaatggc cccacaatgc
     5581 aggtgcgcac aattgactac agaggaacgg atgaaaacaa tcccatagta tctttttcag
     5641 atacaacctt catcaatggt gaacagatgt tgagttatga ctcgcattca tcagggcgag
     5701 tctattccag agaagaatat atgatgtggg aattgcagca acgggtatca gaagcttcca
     5761 gtgcccggac acaggattac tggctgatgg atgcagcggt aagaaacgga gaatggaaga
     5821 tcacaccaga attattacgt cacacaccgg gatatatccg gagtacggta tcgaaatggt
     5881 ccagaggatg gctgaaaacc ggcacaatac tccagactcc agaagacaga aatacggatg
     5941 tatacctgac taccatacag aacaatgtat ttagtcgtca ggggggcggc taccaagtgt
     6001 attatcggat tgatggaatg gctggtgcgg atatagcgga taatgcacca ggggaaaccc
     6061 gctgcaccct caggcccgga acatgttttg aagtgacaag tgtggatgaa aggcattatg
     6121 agtggaatat catttatgtc acgctgaaaa cctgtggctg gagccgaaat ggccaaagca
     6181 aaacgccgaa tggtgacaac ctttttaact aattaaaatc tctgttaaag atgtataacc
     6241 cggcatctat gtgctcacca gcatatagat gcaatgtgat tcccctgatg gtcgtatcag
     6301 agatggtatg cgagcctgta catagatttg tgtaattgcc tgattttgat atgttcaatc
     6361 caacatcaaa agcaggttaa tttatggacg aaaaacagtt gcaggctctg gctaacgaac
     6421 tggccaaaaa tctcaaaacc cctgaagatc tcagccagtt cgatcgcctg ctgaggaaaa
     6481 tcagcgttga ggcagctctc aacgctgaaa tgtcccacca tctgggctac gataaaaacc
     6541 agcctaaacc gggtgccaac tcccgcaatg gctattccac aaagaccgtt atcatcggtg
     6601 atggccccct ggaactacgc actccgcgcg atcgtgatgg ctcttttgaa ccgcaacttg
     6661 tgaagaaaaa ccagacccgg atcaccggga tggataacca gatcctgtcg ttgtacgcca
     6721 aaggcctgac aaccgtaagc gtacagcctg aaccgtctgg tcagaatctg acgaattaga
     6781 gtaagcgtaa accgaccgcc gtatgtagcc attagacaag aattggtaac ttagacgccc
     6841 atctgacaca gacggacatc taagtatgga attacaggac tggcgaaaag aacctcgtaa
     6901 aaagtattcg aatgaattca aacttcgtat ggttgaactg gcatcacaac ctggagcttg
     6961 tgttgcacag attgcacgag aaaatggcgt caatgataat gttattttca aatggctcag
     7021 gctctggcag aacgaagggc atgtttcgcg gcgtcttccg gtaacaacct cttctgacac
     7081 tggcgttgaa ttattacctg tagaaataac gccggatgag cagaaagaac ctgtggcggc
     7141 cattgcgccg tctttatcca cttccactca gaccagagtc agtgccagtt cctgcaaggt
     7201 ggaattccgt cacggtaaca tgacgctgga aaatccatcg ccagagctgc tcacgatgtt
     7261 aatccgcgaa ctgagcggga ggggaagatg atttcactcc catcaggtac ccgtatctgg
     7321 ctcgttgccg gcgttaccga tatgtgtaaa tccttcaacg gactgggaga acaggtacaa
     7381 catgtgctga atgataatcc cttctccggt cacctgttta tcttccgtgg ccgacggggg
     7441 gacaccgtta aaattctttg ggctgatgct gatggtctgt gcctgttcac caaacgcctg
     7501 gaggaaggcc agtttatctg gcctgcggtg cgtgacggta aggtatccat tacccgctcg
     7561 caactggcaa tgctcctcga taagctggac tggcgtcagc caaaaacatc ccgccttaac
     7621 gccctgacaa tgttgtaaaa aacacctggc cgcattataa aaacggccat gagtcagaaa
     7681 tacctcattc gcattgcaga actggaaagc cagctccgtc agaaagacca gcaactgagt
     7741 ctggttgaag agacggaggc cttcctgcac tggcacgtgc cgaagaaaag atcgaagaag
     7801 atgaacggga aatagaacat ctgcgggctc agatagaaaa actgcgccgg atgctgttcg
     7861 gtacccgttc tgaaaaactg cgtcgtgaag ttgaacaggc tgaggccctg ctgaaacaac
     7921 gcgaacagga cagcgatcgt tacagtgggc gggaagacga tccacaggtt ccccgccagt
     7981 tgcgacagtc gcgccatcgt cgtccgttac cggcacacct tccccgtgaa atacagcgtc
     8041 tggaatctga agaaagctgt tgcccggagt gtggcggtga gctggattat ctgggggaag
     8101 tcagtgcaga acaactggaa ctggtgagca gcgccctgaa agtgatccgc acagaacggg
     8161 taaaaaaagc gtgtacaaaa tgtgactgca tcgttgaagc accggcgccg tcccgcccga
     8221 tagagcgtgg tatcgcgggc cccggattac ttgcccgcgt gttaacggga aaatactgcg
     8281 aacacctgcc actgtatcgt cagagtgaaa tctttgcccg acagggtgtc gaactgagcc
     8341 gtgcattact ctccaactgg gttgacgcgt gctgccagtt aatgacgccg ctgaatgatg
     8401 ccctgtaccg ttatgtgatg aatacccgca agcttcacac tgacgacaca ccggtaaagg
     8461 tactggcacc gggcctgaaa aagacgaaaa cagggcgcat ctggacgtat gtccgggatg
     8521 atcgcaatgc gggttcgtca tctcctccgg cggtctggtt cgcgtactca ccgaaccggc
     8581 aggggaaaca cccggagcaa cacctccgcc ccttccgggg tatcctgcag gcggatgcgt
     8641 tcacaggtta tgacaggctg ttcagtgcag aacgtgaagg tggtgcgctg acagaagttg
     8701 cgtgctgggc ccatgcccgg agaaaaatcc acgatgtata catcagcagc aaaagtgcga
     8761 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
     8821 gattaccaga gtcagagcgt cttgcagtca gacagcagcg aagcaaagcc ttactgacgt
     8881 cgctgcatga atggatgatg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
     8941 aagcgttcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggac
     9001 tggcggagac ggacaataac acagcggaaa gagcgcttcg tgcagtctgt cttggaaaga
     9061 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctatacgggc
     9121 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
     9181 gcgtactgcc ggaatggccc tccaaccggg ttgatgaact cctgccatgg aacgtagtac
     9241 tcaccaataa ataagcgtca atacggtgct ccgttgacgc ttaccttctt tcattcgctg
     9301 aaggtggaat gtatccacgg ggaacgcttt agcagccggg aaataatgcg ggaaacggtg
     9361 tttaattata tcgagtgtga ttacaatcgc tggcgtcgtc acagtgcctg tggcggtctc
     9421 agcccggact gtcttgcact gcaaatattc agaaatgagt ctatgcagtt caccagtaaa
     9481 atcagccatc agagagggaa tggccttatt aatgccctgc cctggtatta attttaattg
     9541 taataattta atttcaggat gtgtagctgc agcccgatac agagttgcaa ggacacactt
     9601 ttgccagagg gcgttacagg aaagcttaac atttgattct gtatacataa taaatcacct
     9661 tacagttaca acaacaggtc aaaaaccgct gtagccagag ttacgctggc ctgatgcttt
     9721 agtgtcgagc ttcgtcagat aatccagacg tgccaataag cgctgatact gctcagggaa
     9781 atcaggatcg ttaatatcca ggatgtcacg tccattagca gggaaatgaa caatgcagcc
     9841 ccctggatta actatgcaga aatcgtcctg aggcactgat aaatacgaga ggactctcgc
     9901 gtatggttga ttgacaccac agtgcgaatt cggcgaatgc gagatcacgg tacgattccg
     9961 caccacacta ccaatcaata ccccgggttt tattcaggta agcaggactg cggatatccg
    10021 gtgtcgcgcc tttgtgtcac gaacggggca ggtgcgaaac accggataaa atgcaggttg
    10081 cgatacctct gaacgccctg cgcagagcgg agatttcgga ttaagtactc gcacctccgc
    10141 agtcctcaaa caagtctggc tggtagctgt aaacagactt cgtacatacc gctctgcaat
    10201 aaatccccgt accacaggcc tcacagaact tcttcccatg aaaaaactgc tcacacatca
    10261 cacaatgcca ctccagcacg accggtaatg gcgataaaaa catcgccata tcctcaatgt
    10321 aagggtggga cttctccgga ttcagcacca cacagtccgc cttctgttgc tcgctcaggg
    10381 catgtaaatc gtgctcaaac cacgccccct gagcatctgt ctgcagaatc aaccggccat
    10441 gacaggaaag gcagaaacac tgccggatat ttctgctgag cgcagagtcg gcactaataa
    10501 tgtgctcacc cggcgtgatc cccagccccg tttttatacc gttcattctg ctcccccctc
    10561 ctcactgaag cgcccggtct ggcagtgtgt gcagtaccgc tccccataat aatcgtggtg
    10621 acattgcctg cagtgccagc tggctttacg tactacgggt aaggcatccg gtacgaattg
    10681 ctgcagacgt ttaatcagtt gtatctcctt gcgctccggt ctgacataag gacactgttg
    10741 accgtgctct gtcagcccgt cgtcagtgtg ttcaaaccag ggcagttcgg tgtcgtattg
    10801 cgggtggtat ctgagtgcac tgccacacaa gtggcaggtg tagtgggcat aaggagcagt
    10861 atgtgcggta cgggcccccg tcagacgtcc gttaccatca agtgcgagaa aggattttgc
    10921 gtacatagta tatattcctc agccgccaga cgacacgcag gcaacagtcg tcccggtacg
    10981 ggcagcgtgg tcaggtgtga accgccccgg aaatcctgga gactaaactc cctgagaaag
    11041 aggtaaacag gatgactaaa aatacacgtt tttcccccga agtccgtcaa cgggcagttc
    11101 gtatggttct ggaaagtcag ggcgaatatg actcacaatg ggcggcaatt tgttccatta
    11161 ccccaaagat tggctgtacg ccggagactc tgcgtgtctg ggtacgccag catgagcggg
    11221 ataccggagg cggtgatggt gggctcacca ccactgaacg tcagcctctg aaagagctgg
    11281 agcgtaaaaa tcgtgaactg cgccgcagta acgatatcct tcgccagact tccgcttata
    11341 tgcagtgaac tgcatattgc cccgtcaacg tattaccact gtcagcaaca gcgacatcgt
    11401 cctgataaac gcagtacccg tgcgcagcgc gatgactggc tgaagaaaga gatactgcgc
    11461 gtatacgatg agaatcatca ggtatacagt gtacgtaaag tctggcgtca gttgttacgg
    11521 gaaggtatca gagtggccag gtgcactgtg gcacgtctca tggcggttat ggggcttgcc
    11581 ggtgttctcc ggggtaaaaa ggtccgcact accgtcagcc ggaaagccat taccgcaggc
    11641 gaccgcgtaa accgtcagtt cggggcagaa cgtcctgacc agttgtgggt ggctgatttt
    11701 acttacggtg tgcccggagt tcagggcggg catggatgct taaatgaacc gcgagtctgt
    11761 ctggaatatt gaaccggtaa ctcacgatga gaaacccaac aatcccaccg ggtgtgacgg
    11821 tggagaacct gagcggcagt gacctgcggc atgcccgcag ggtgatgtaa cccgctgaca
    11881 acggggattg aggcgagatc actaagccga gatgatcctc aaggttaagt actgaaaggt
    11941 tgaagaacat gaacccgtta atccgcctct gtgggttgaa aacgtcacca cggcctacgt
    12001 gatctgacag gccgtgcagg aggaactggc agtgatacgt aagcactgcc ggtcgaaggt
    12061 gttttgacat gtatgcgaaa caccggggca gcagcgtcta tcacgcttgc gttgctgact
    12121 tctgccaact tgcggcaagc aaggataaag agtgcgacgg gcagcctcct cagtatgcct
    12181 gagcccaggc aggtaaaccg gggaaggtca gcgacggatg ttaagggggc atggctccga
    12241 tgacgcgctg gctggcggag cttccgtagt agtccgcgat ggggaaagcc cattacatgg
    12301 cgaagggaag cagtttgaat gtgtttgcga cgtgaattaa ctgacctaac gaggtgaaga
    12361 cctttgataa tcagcgaaat gcaacgcaag ctggccacat gggcagccac cgatccgtcc
    12421 ctacggattc aacggctgct gcgtctgata acacaaccag aatggctggc tgaagcggcg
    12481 cggatcacgc tttcatcaaa gggggcccat acccccggcg ttgatggcgt gaacaaaaca
    12541 atgctacagg ccagactggc tgttgagctg caaatcctca gggatgaatt actctcaggc
    12601 cactaccagc ccttgcccgc cagacgggtt tacatcccta aaagcaacgg caaactgcga
    12661 ccactgggta tccccgcgtt gcgggatcgt attgttcagc gggccatgct gatggcgatg
    12721 gagccgatat gggagagtga ttttcatacg ctctcatatg gcttccggcc tgagcgcagt
    12781 gtccaccacg cgatccgcac ggtgaaatta cagctcacag actgtggtga aacccgggga
    12841 cgctgggtga ttgaaggcga cctgtccagt tacttcgaca ccgtacatca tcgactgctg
    12901 atgaaggctg tacgccgcag gatcagtgac gcacgtttca tgactctgct gtggaaaacc
    12961 atcaaggcgg gacatatcga tgtcggtctc tttcgggcgg ccagtgaagg tgtgccacag
    13021 ggcggtgtta tatcgccgct attatcgaac atcatgctga atgagttcga tcaatacctg
    13081 catgagcgct acctgagcgg gaaagccaga aaagatcggt ggtactggaa taacagtatc
    13141 caacggggcc gaagtacggc ggtcagagaa aactggcagt ggaaacccgc ggtggcgtac
    13201 tgccgctatg ccgatgattt tgtcctcatc gtcaaaggca ccaaagcaca ggcggaagcc
    13261 atcagggagg agtgtcgggg tgtgctcgaa ggcagtctga aactcaggct gaacatggat
    13321 aagactaaaa tcacccatgt taatgacggc tttatctttc tggggcacag gctcattcgc
    13381 aaacgcagtc gttatggcga gatgcgagtg gtctcaacga tcccgcagga gaaagccaga
    13441 aacttcgccg catcgctgac agcactgtta tcaggcaact acagtgaaag caaagtcgat
    13501 atggctgaac aactcaaccg aaaactgaaa ggctgggcca tgttctatca gttcgttgat
    13561 tttaaggcca aagtcttcag ttatatcgac cgtgtcgtgt tctggaagct ggctcactgg
    13621 ctggcccgca aataccgtac aggtatcgct tccctgatga ggtggtggtg taaatcaccg
    13681 aaaccgggtc agagcaaaac gtgggtttta tttggtaaaa ccaaacacgg caagctcagc
    13741 ggcgaaatac tgtaccggtt ggtggggcaa ggcaagaagc tgttccgctg gcggctaccc
    13801 gaaggtaatc cctatctgag gacggggacc a
//
LOCUS       DAZFYC010000128        13719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFYC010000128 DAZFYC010000000
VERSION     DAZFYC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.128"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(170..658)
                     /locus_tag="ACHKW5_004178"
     CDS             complement(170..658)
                     /locus_tag="ACHKW5_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7513443.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            complement(671..3478)
                     /locus_tag="ACHKW5_004179"
     CDS             complement(671..3478)
                     /locus_tag="ACHKW5_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7513444.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARCLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETPFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLASNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEVDRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATIRSLSLVIGVLYGKLATLRLGFDILTRS
                     MNVVRVLPALWGMLTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFDPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILRD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(3465..3620)
                     /locus_tag="ACHKW5_004180"
     CDS             complement(3465..3620)
                     /locus_tag="ACHKW5_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO7513445.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(3629..4003)
                     /locus_tag="ACHKW5_004181"
     CDS             complement(3629..4003)
                     /locus_tag="ACHKW5_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7513446.1"
                     /translation="MKDIDTETRNNTVADDVTAGEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFMLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4059..4571)
                     /locus_tag="ACHKW5_004182"
     CDS             complement(4059..4571)
                     /locus_tag="ACHKW5_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO7513447.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNERPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(4571..5755)
                     /locus_tag="ACHKW5_004183"
     CDS             complement(4571..5755)
                     /locus_tag="ACHKW5_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO7513448.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            complement(6178..6261)
                     /locus_tag="ACHKW5_004184"
                     /pseudo
     CDS             complement(6178..6261)
                     /locus_tag="ACHKW5_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6222..7022
                     /locus_tag="ACHKW5_004185"
     CDS             6222..7022
                     /locus_tag="ACHKW5_004185"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO7513449.1"
                     /translation="MTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQI
                     SEMLMDIEIDHADQTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLP
                     SFAITRSSGDRHQFRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPTKPKKEKSSSR
                     EGDYMEGAEGNVFVLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEM
                     HGTVTGFKSEIDNQDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            7065..7325
                     /locus_tag="ACHKW5_004186"
     CDS             7065..7325
                     /locus_tag="ACHKW5_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO7513450.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            7515..7655
                     /locus_tag="ACHKW5_004187"
     CDS             7515..7655
                     /locus_tag="ACHKW5_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7513451.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(7845..8126)
                     /locus_tag="ACHKW5_004188"
     CDS             complement(7845..8126)
                     /locus_tag="ACHKW5_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513452.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRTGGYDVVCYESDVTDEMIRQWKQENAE"
     gene            complement(8667..8753)
                     /locus_tag="ACHKW5_004189"
     tRNA            complement(8667..8753)
                     /locus_tag="ACHKW5_004189"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8717..8719),aa:Leu,seq:taa)
     gene            complement(8766..8839)
                     /locus_tag="ACHKW5_004190"
     tRNA            complement(8766..8839)
                     /locus_tag="ACHKW5_004190"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8805..8807),aa:Cys,seq:gca)
     gene            complement(8894..8969)
                     /locus_tag="ACHKW5_004191"
     tRNA            complement(8894..8969)
                     /locus_tag="ACHKW5_004191"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8934..8936),aa:Gly,seq:gcc)
     gene            complement(9121..9669)
                     /gene="pgsA"
                     /locus_tag="ACHKW5_004192"
     CDS             complement(9121..9669)
                     /gene="pgsA"
                     /locus_tag="ACHKW5_004192"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO7513453.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9726..11558)
                     /gene="uvrC"
                     /locus_tag="ACHKW5_004193"
     CDS             complement(9726..11558)
                     /gene="uvrC"
                     /locus_tag="ACHKW5_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO7513454.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11555..12211)
                     /gene="uvrY"
                     /locus_tag="ACHKW5_004194"
     CDS             complement(11555..12211)
                     /gene="uvrY"
                     /locus_tag="ACHKW5_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO7513455.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12353..12424
                     /locus_tag="ACHKW5_004195"
                     /pseudo
     CDS             12353..12424
                     /locus_tag="ACHKW5_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            12507..12683
                     /gene="yecU"
                     /locus_tag="ACHKW5_004196"
     CDS             12507..12683
                     /gene="yecU"
                     /locus_tag="ACHKW5_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO7513456.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            12670..12894
                     /gene="yecF"
                     /locus_tag="ACHKW5_004197"
     CDS             12670..12894
                     /gene="yecF"
                     /locus_tag="ACHKW5_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO7513457.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(12961..13683)
                     /gene="sdiA"
                     /locus_tag="ACHKW5_004198"
     CDS             complement(12961..13683)
                     /gene="sdiA"
                     /locus_tag="ACHKW5_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO7513458.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         3444 a         3758 c         3258 g         3259 t
ORIGIN      
        1 agtaagacag cacgaaaatc tccttaatta ttatttcaga ccacacatgt tataaggtta
       61 agagattata aaatcctgtt atttgttatt caaaaacaat tttctgagaa ggacatacaa
      121 cagcaagtcg ccagtcacct tcatcaggaa attggcgaca tacgttaaat cagagcagcc
      181 ccttaactga gctggccgcg ctattaaggg atgatgtcac cttatctttg aagccggaca
      241 acatatcgct gaacgatgag gattgcaggc gctcccgcaa atcctcatca cagcgttcaa
      301 gagtcagtga aaattctatc tttttcgcct taccgtagcg atcaaactcg gagcgggtcg
      361 tattcgtttc agtcaggaca tacatgccgt aaatctgccc gacgccatca atcaaaggcc
      421 agggtcgtcc tgtatacgcc tgcgtggtca gcagcgacag cgacacttcg ccacctgtaa
      481 tttcaggata aagcacacca gaaagaacga tgcgatcatc acctgcaccg atatactgcc
      541 aacttgctga acggttaacg cgttcatttt tcacatgccg ccagcttttg ttttgctgta
      601 actgctgatg cggcagcgtg cgcagctcaa aaacaaacat gccgtagatc atcatcatgg
      661 ccatgactcc tcaatcttta tcgtaaaaac tgccacgccc ggcacgggca cgccgttcca
      721 tttctgccct gaccatttca ccgaccagtt tcgccagttc gcggggattc tgcgtaacaa
      781 cgttatgcag atgaacatga atttcaccac caaatccgga ggcaacaggc tcccggttac
      841 gggaagttac aggaactgat gccactggag atcgtatggc ctccgccacc gggcgggagc
      901 tggccgcaac aacagggacc agcgccggag gcagcggtgc cgggaccacg ggggtgatat
      961 taattgcggg ggcaggctta ctgacctgcg caatcttccg ctcctgccac tccccacgaa
     1021 cagcaagtgc gcggggcagg ttcttaaaga caatatcgcc ggggccaatg cgttttttcg
     1081 tctcatcaac cagcttacct gtgttatcag caattttgct gagtctgcgt agcgtcccgg
     1141 tattgctgtc tgtgagcggt ttgttgtctt tgggtttatc acctccggtg ccattgccat
     1201 tttccacagg cttcggcgga ttgattttcg ccaggtcccc ctgaagtaag gcaaccttgt
     1261 cccgaagaat ggccgcacgc tgtgcgtctt cgattttctt gcgcgccctt tcagcttcat
     1321 ccggaaggac gccaagtttt tcaagtatcc acgccagcgt atccagtagc atttttgcag
     1381 gtgtcagaac aagttgtaac gcaccgccaa gaacgttacc gaatatctcg ccagcactgg
     1441 tacatttatc cagcgtttcc ttgctggact ccatcggtga cagcagcgat ttaaaccagt
     1501 taaacacctg gctgatcccg cttccgattg cgtcaaaaac agggtcaaac cgttcaaagg
     1561 tttcgcgcaa cggggtcagc ctttccataa tcccgctgaa caccccggca aaaaatgccc
     1621 tgatgggatc ccagtatttc cagataagaa cggcagctcc ggcaagcgca gccacgataa
     1681 gaccaaccgg actgaacagc gccccgatag cgcctcccag caaagaaacg gaacccgtca
     1741 gcattcccca cagcgcaggc aacaccctga cgacattcat tgaccgggta agaatgtcaa
     1801 aaccaagacg cagggtggcc agctttccgt aaagcacccc aataaccagc gacaacgagc
     1861 gaatcgttgc agtcattgcc agcaacgcac cgcctgctat cagtagctgg cgtgtcagta
     1921 ccggatgggc ctgcgccagc gaggtgattt tttcaagcac ccgcgtgagc cactgcgtga
     1981 cagaacgcag cggaccgtca accagatcac tgatgcgaat acgaagacct tcccatgcgc
     2041 tgtcgagatt tttcaggtcc ccatcaagat tatcggccat tacttttgcg acgcgatcga
     2101 cctctcccct tgccccctgc aattctctgg tcagtttttg cagctctcct gaaccagccg
     2161 ccgcaacaag cgtctgcaaa ccaacgaacg cctcttctcc ggcgatgtcc ttgaagaagg
     2221 aaacctggtc cacctgtccg tatttttgtg tcgccttata gagatcaagc agcacatcct
     2281 ccatcgggcg cattttgccc ctggcgtcag caactgacac ccccagctct ttcagcgcat
     2341 cagccgcagc ttttggcggt gatgcaaggc gggacagact tgcgcgcatg gccgtaccag
     2401 catcgcttcc acgaagacca ttactggcaa gcatcccggc catggctgcc gcttcttcaa
     2461 gactgatacc aagttttgcg gcaaccggac cggtatactt catggtttcg cccagcgcgc
     2521 gtaaatcagt attggtccgg gtgaatgccg ctgtcagcgt atcgccaacc cggtccattt
     2581 gatcggctgt caggttgaac tgtgtgagga tattggagcc tatatcagcc gtctcgccga
     2641 gttcgacgcc acctgccagc gccatattaa gaacaccggg caatgcggcc tgaatggcct
     2701 gcggagtaaa accagccatt gccagaaagc tctgcccact ggcggcatca ctcgcagtaa
     2761 acggtgtttc agatccaagt tttaacgcct gctcacgcag cgccttaaac tgcgggctgt
     2821 ttttgtcgat tcgcgtcagt gcctgaacgc gggacatctc tttgccgaac ccgatcgcag
     2881 gctgcaaaaa acgcccggca gcatagccgc ccgccgctgc cgcaccaatt gccagtgcac
     2941 cacctgtttt cagttttccc gctgtttcct gcgcgcgcga ataccgctca cgcgcccgcg
     3001 ttacacgcgc aagcgcctgc cgttcgcgtt caagctggtt gttgtactgt tcggtgcgtc
     3061 tgatggcctg ctggatggtg ttatcgctgc ctgtcaggga aatgccgtgg cgtttcagct
     3121 ctccgccaag ctcccgcatt ttttgaattt cccgtgtgcg cgattcattc aggcgttcaa
     3181 gccgggtgct taactgctgc atcagctttt gttgtttttc gctgagcact gtacccgtac
     3241 gttgtaactg attaagggcg ttaagctggc gtcgtgcttt cacgatgcca gcatccgctt
     3301 tactgacagc gtcacgggcg cgctcaaatg aacgcgcctg acgctcgaga tttttaatcg
     3361 ccccttgcgt tcgctggatg gagtcaccaa actgccccat caggcagcgg gcgttttcgg
     3421 caggccgggt cagcctgtca acggcgctga aagcgacccg gatatcaaga gtcttcatta
     3481 tctgcattcc cgctgcgaag tgccgcccgc tcgcgccagc taaccacttc gccgggcgtc
     3541 atcatgaaga tttcggcggg cgaccagtta aaaatggcgg caatatccgc caccagatct
     3601 tcgatgtgct caaagcacac cagggtgatt acgctgccgt ctcctgcacg ctcttcgcgc
     3661 cagagtctgg ctcgctcata aaatttacag ccacagcgca caactgaata aaatcgcgtg
     3721 acgacatttt tttaatcatc acttcatcca gtcgtggcga ggtcacgcga ggcaacagca
     3781 taaacatggt atccgctttc agattcagca catcagacag cgacagacca cgcagggatc
     3841 cagcctgctc aatagccccg gtgatctcca catacgtgat tttttcgcca ccacgctcaa
     3901 ttggtcgggt cagttttacg ccacgttcga cagccatatc ctcacctgcc gtcacatcat
     3961 ccgccacggt gttattccgg gtttcagtat cgatgtcttt catcagttgt ctccttttca
     4021 gtcagaggcg acgcactgcg ccgcctgcat attacttatc agccaagccc aagcgcggaa
     4081 cggatacggt caggcacaat gtccttgccg tccttccggt agatgtggtt caacaggtcg
     4141 atttcccaca gcgggcgctc gttaacgctc agcttgtagt aggtgttttt gacagcgtaa
     4201 gtgtgtgatg tggcttcccc ctgtttggct tcccccatat caatttccgt cacacgcccg
     4261 cgcatctcga tctcatacag atcgctttct gcatcggtgt agtattcacc cgcaaaacgc
     4321 agcagcgtgc cgtcaatcgt gccgccatat ttaaggaaca gcgcacgaac agctcccccc
     4381 ataacaaaac tcgcatcaag cgcggagtcg tccagaccga gatcaatact taccgccccc
     4441 atcatgccac caccacgata gctgtcggtt ttgcgcgtca gtttgggcgg cgtgacggat
     4501 gtcactttgc ccacttcgtt ttcaccatcc acaaacaacg taaaaaagcg aagatgtttt
     4561 ggcacagcca tcaggcacct cccagcaccg caaatgcggg accaaagaat tcatcggtaa
     4621 acgtctggta aagctccatg tcttccagtg gcggaacggg cgtatatttg tagcgaatac
     4681 gcacacgtcc ctgacgtaaa tccgtggtgc cgttatccac cacgtcatac cagcactccg
     4741 caccaatcag tttcccggca gtaaccagcg aatccagttt tgccctgatg gcactgataa
     4801 catccttcac gttcgcaggc gtcagtggac tgtcgatggt ttcaaactgc gcttccgcaa
     4861 ttgaatcagc cagcacctgt gcggttcggg tatacacctc aaagatgtag gcgttcgttt
     4921 ccggtgtgcg gttgccccag aagcggaacc cgttgcgacg aataatggtc gtgatttctt
     4981 tgttattgag gctgttggca tcactgtctt cggcctgcaa cgaccagaac acatgcctgg
     5041 acattcccag cacattttta accggaacat tggacagcga tttgtgccag ccctgctcat
     5101 ggtcaatgta cgcacgaagg ccgcacgcat aagcaggcgc ggggaacgtt tcgtttttgc
     5161 cacttttcgg gttgtaggcg atgaagtcag gccataagag catcacctca cgttcgttga
     5221 atttctggcg gtaggtaatc gcctcagcca tcgtgttaca accatgacat gtggcataca
     5281 caaacgcgcg cagtttaccc gcaatcacgc acagggattt tgtcaccgcc tccgtgtcca
     5341 gctccggcgc ggccagaata cgcggacggt atccgatgct ttcatcctgc tctgcaacaa
     5401 gcagcgcata catccccgta tagctgccgt catcctcaga accaccgata accagttgat
     5461 cctgcgtctt accgtcttct tctttgtgtt cagccacgcg aacgacgatc acctttgtgc
     5521 tcacctggtc tgcgatggcc ttaagcgcac gataaagcgt ccccgttgtt ccgcattttc
     5581 ccagcacgtc attgacgcgg gtcagcagtg taggcttgtt cagcgggaac agcttcgcgt
     5641 ccgcatcatc cgccgttgcc acgataccga taacgctgga atcaacatcg ttaatcgctg
     5701 ttaccaggtc ggtattttcc gtaacacggg caccatgaaa acgagtttca ctcatagctt
     5761 cagccccttg tatccgttaa atgattcggc aacaatcatc acccaccacg cgcgtaatct
     5821 cacccctgcg ccgttctccc gacccggcga caacaaaaag cagtaacccc ctccgcacgc
     5881 acatgcgacc atgccgcaca gggagggaac agatgaccga caccaccatg caattgctca
     5941 gtcagggcac agaccccgta aaaatgccgg attttgatat tctcgcggag ggtaaaacgc
     6001 tgtccggcgt ggcagagcgc ctgatgagcc tgtcgctgac cgacaaccgg ggatttgagg
     6061 cagaccagct caccatcacg ctggatgatg cggatggtca gttgcggcta ccgccacggg
     6121 gcgcgcgcct gacggttctc attggctgga aaggcgaacc gctgacagaa aaaggctctt
     6181 acattgttga tgaaatcgca cacgaaggac cgccggacag gctgactgtt tcagccagaa
     6241 gcgcagattt tcgggatgaa tttaacgtta aacgtgaggt gtcctggcat gatgtgaccg
     6301 ttgagcgtgt ggtatccgcc atcgctcatc ggtacggtct gaaaccgcaa atcagcgaaa
     6361 tgctgatgga tatcgaaatc gaccacgccg accagaccga agaaagtgac atgtccttcc
     6421 ttacgcgcat ggcggaaatg ctgggcgcaa tcaccacggt aaaaagcggt aatctgttat
     6481 tcatcatgcc cggcggtggc gtgaacgcac agggccagcc gttgccatcg ttcgccatca
     6541 cacgcagcag cggcgatcgc catcagttcc gcattgctga ccgcgaggcg tatacggggg
     6601 tacgcgctta ctggcttgat cttaattacg ggaaaaagaa aaaagtcagc gtgaaacgcc
     6661 gcaaaccgac aaaaccgaaa aaagagaaaa gcagcagccg tgaaggtgat tatatggaag
     6721 gcgcggaagg caatgtgttt gtgttacgca agacttatca gaacgagcag gcagcaagac
     6781 gcgcagcggc ggcaaagtgg caacaactac aacgcggagc cgcatcattc tccatcacgc
     6841 tggcacgtgg acgagcagaa ctctaccccg aaatgcatgg cacggtaaca ggatttaaaa
     6901 gcgagattga taatcaggac tggattattg caaaagccga gcacaccatt gataacagcg
     6961 gctttaccac gcagcttgag cttgaggcaa aaatcccgga atggatagcg gaaacagagt
     7021 gagcaactta gaatagcggc agcaccacgt taaaggaggt cgctatgttc cgttgtccgc
     7081 tttgtggcgc atctgcccgt atccgcacca gtcgtccgga aaatgattca aacaccgtgc
     7141 ggcaaaagta ttaccagtgt aacaacctgg aatgcggcgt atgcttctca acactggaag
     7201 ctttccataa attcacatcg aaacacgcct ccggcgttca ctcatcagaa ggtatcccgt
     7261 ggcatgagct gccagcttca cacaggggaa acaatcagat gagtttgcct ttacctcaga
     7321 attaacaggc agaattgccg gagtaacaaa aaagcgatag attacgtgcg ggtgcctttc
     7381 ggctgatggt cggagggaat acccgaaggc cggatgtgga aaggccccgg aaaacatttc
     7441 tgtttaaccg aggccctaac ccgtcaacct agcaagtgat aggttagcgc ctctccaaca
     7501 aaggagcaag cgctatgtcg caaaaatcgc ttacggccat cacgttctgc gtgacggtaa
     7561 tcctcatcat ctggatgctg cacggttcgc tgtgtgaaat acggatgagc ttctggggag
     7621 cggagtttgc ggcgttctta cagtgtaagc agtaaggaaa ccgcgacggg gaggcaactc
     7681 cccgtcaatc ggttgctagg gtaaggccga taaggcaccc tatctcatag gcatgaataa
     7741 caaccccgca gcgtaaaaac tgcggggttt ctttttggaa ctctcactag ctagtgagcc
     7801 gttaactgca cataatgctc gcacaacaaa tcagttaaaa gagattactc agcattctcc
     7861 tgcttccatt gccggatcat ttcatcggta acatcacttt cataacatac cacgtcatac
     7921 cctccagtac ggctatatgc actgcgtcca ccacatctac taccattcct tgcatgatta
     7981 tacggacacg cacaattgcc tggataagat tcaatggatt ctttaattat ttctttcttg
     8041 atctgagcat ctgactttcc tgttgctgca tacccactaa aagataccaa acaaaggcag
     8101 attgccatta ataaaccaca tcgcataccg atacactcta ttactaacca atcatagccc
     8161 acatcataaa atcgagatga ggagtacaac acctttaaca agaaggtgcc atcacgataa
     8221 tatgccgtgc aattcatcaa aaaaaagatc tatatcaaag agatagagtt attatcagtg
     8281 ctgcagacat cagcattcga ggcagcagaa agacagtaaa tctaacgaat ttgtcttttt
     8341 ctcaggatgt tgtcagatta aaaaagatag agtagcgcag acaccttttg gtggatggta
     8401 ggaggaaaaa tgaagttgag tgggaagatc ccagaaaaaa ctttggttta accaaggccc
     8461 tagttcaata tgcgaaatgc agatgagttg caggagagtt ttgcttcacc cttagagttt
     8521 aaacagacgt gtactatcat tatttctgca tatcgccaca ccatcgccac tttaccgcca
     8581 tttaacaaaa tacaaataca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     8641 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     8701 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     8761 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     8821 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     8881 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     8941 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     9001 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     9061 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     9121 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     9181 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     9241 ccacagcagc caggccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     9301 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     9361 aatttcacgg gcgatcatcg ttgccgccgg tagggtcacc caccagctgt gataatgctc
     9421 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     9481 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     9541 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     9601 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     9661 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     9721 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     9781 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     9841 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga gctggtattt
     9901 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcgtgtga ttcatcgcga
     9961 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
    10021 ggctcaaaga acagtgtttc cagcccagcc ttacgatcta ctcctttggc aacgccaagt
    10081 agcagtggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
    10141 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
    10201 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
    10261 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
    10321 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
    10381 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
    10441 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
    10501 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
    10561 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
    10621 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
    10681 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
    10741 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
    10801 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
    10861 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
    10921 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
    10981 tcgacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
    11041 cgccctatct ggtattgcag acacggacgc gagcgattgc gataaacact attttcgcac
    11101 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
    11161 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
    11221 gggtgggtat caccactcag gaagataaaa ggatatgatt tatcatcgcg tagcaaaacg
    11281 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
    11341 gtgtgagtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
    11401 gaagcgaggt tgctacggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
    11461 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
    11521 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
    11581 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
    11641 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
    11701 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
    11761 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
    11821 gagcgatgtc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacaa
    11881 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
    11941 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
    12001 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
    12061 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
    12121 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
    12181 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
    12241 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
    12301 tggtgggtat tcgatttttc tctcgttttt gtcaattcga ttgtccacag ttatggtcac
    12361 gcccgtcacc aacggcttac cggatcgttt cacagtaagc cctacgatga aatgtgacaa
    12421 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
    12481 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
    12541 tattttatag cactgccgat atcacgctca aaaaacaacc actgctattt ttagcaaagc
    12601 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
    12661 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
    12721 gaagtctcct gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg acaagctgac
    12781 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
    12841 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
    12901 accggctgag agcgtgcctt tcagccggtt tttgcatctg gcacgcagaa agaaaagcga
    12961 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
    13021 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
    13081 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
    13141 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
    13201 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
    13261 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
    13321 ctgagtgaca ccgcggcgta aaccatgtgc gcgcgcggct tcccataacg gctgtgcttc
    13381 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
    13441 cggatcaatt gcgagaaagt tttttgcctg ataataacta acccacgcct cagggtaatt
    13501 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
    13561 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
    13621 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
    13681 catagtaaac cgcaacgccc ctgagagtga gtatcattt
//
LOCUS       DAZFYC010000129        13684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFYC010000129 DAZFYC010000000
VERSION     DAZFYC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.129"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..982
                     /locus_tag="ACHKW5_004199"
                     /pseudo
     CDS             1..982
                     /locus_tag="ACHKW5_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(1135..2784)
                     /gene="yjcE"
                     /locus_tag="ACHKW5_004200"
     CDS             complement(1135..2784)
                     /gene="yjcE"
                     /locus_tag="ACHKW5_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO7513459.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2935..4284)
                     /gene="ghxP"
                     /locus_tag="ACHKW5_004201"
                     /gene_synonym="yjcD"
     CDS             complement(2935..4284)
                     /gene="ghxP"
                     /locus_tag="ACHKW5_004201"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO7513460.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4830..5294)
                     /gene="soxR"
                     /locus_tag="ACHKW5_004202"
     CDS             complement(4830..5294)
                     /gene="soxR"
                     /locus_tag="ACHKW5_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO7513461.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5380..5703
                     /gene="soxS"
                     /locus_tag="ACHKW5_004203"
     CDS             5380..5703
                     /gene="soxS"
                     /locus_tag="ACHKW5_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO7513462.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5706..7292)
                     /gene="pdeC"
                     /locus_tag="ACHKW5_004204"
     CDS             complement(5706..7292)
                     /gene="pdeC"
                     /locus_tag="ACHKW5_004204"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO7513463.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7722..8003
                     /gene="yjcB"
                     /locus_tag="ACHKW5_004205"
     CDS             7722..8003
                     /gene="yjcB"
                     /locus_tag="ACHKW5_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO7513464.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8102..8638)
                     /gene="ssb1"
                     /locus_tag="ACHKW5_004206"
     CDS             complement(8102..8638)
                     /gene="ssb1"
                     /locus_tag="ACHKW5_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO7513465.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8892..11714
                     /gene="uvrA"
                     /locus_tag="ACHKW5_004207"
     CDS             8892..11714
                     /gene="uvrA"
                     /locus_tag="ACHKW5_004207"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO7513466.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11749..12105)
                     /gene="yjbR"
                     /locus_tag="ACHKW5_004208"
     CDS             complement(11749..12105)
                     /gene="yjbR"
                     /locus_tag="ACHKW5_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7513467.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12109..12525)
                     /gene="yjbQ"
                     /locus_tag="ACHKW5_004209"
     CDS             complement(12109..12525)
                     /gene="yjbQ"
                     /locus_tag="ACHKW5_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO7513468.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12636..13349)
                     /gene="aphA"
                     /locus_tag="ACHKW5_004210"
     CDS             complement(12636..13349)
                     /gene="aphA"
                     /locus_tag="ACHKW5_004210"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO7513469.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3604 a         3523 c         3297 g         3260 t
ORIGIN      
        1 acgatagaat tacgagaaga gtttacctta ataaagataa ggaggtgtca attaaggtgg
       61 tatattttat aaataatgtc accgtccata ataatactat cgatatccca caggcagaaa
      121 atggtggtta cgatttttca caccttagcc tgaaaggtat cgtgattaaa gatgaagatt
      181 tatccaattc gaattttgca ggttgcagac tacaaaactc tattttccag gactgtaata
      241 tgtatagaac gaattttatt gcgccataat ggaaaaaata ctttttgata attgtattct
      301 cgatgactca tatttcgctc atgtaaaaat gactgacggg actctaaatg catgctccgc
      361 tatgcatttt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
      421 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
      481 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
      541 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
      601 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
      661 taccggctct aatatggcta aggttgattt taatgatgcc aatttaagca actgccattt
      721 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
      781 cgatgaggct agcgtccaag gaatgggcat ttccattctc cgtggggagg aaaatatccc
      841 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
      901 tactggcatg tcacaaactg aggataatat taatgcagcc gctatgaaga ttactgcaga
      961 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
     1021 gaggataact tatttgtttt agaatcatga atgttagccc gagctaacct gttaaaggtg
     1081 caaaagattg cacctttttc agattacctc gggcctggtt acgtcgcttt cggcttactg
     1141 attttcctca attagcaacg cttcaagcaa atcgagatcg tgcagtaatt tttgcagcgt
     1201 ttcgttgctg atctcccgcg tggcgcgcag gtggtacagt tcagcacgtt cagaacgcaa
     1261 tgccgccaga cggaagcgac gctcaaggtt ctcttcctgc acggaacttt caacgtcgtt
     1321 acgtccatcg gcgcgacgac gcaggttacc aatgacgcga gaactgacct ccgtaagcag
     1381 ctggttatcg atattctctt cggtatccgc cgccagacgc tcctccattt tctggatcgc
     1441 cacaatcgcc acttctgccg ttgccgctcg cgcaatacgt tcttctttca attgttgcga
     1501 atggtccgcg acttcaatgt gttgtagcag aataggcaac atcaccacgc cgacaaacag
     1561 cgagaagaga atgacgccag ccgccaggaa caccagctca tagcgcgccg ggaagacgtt
     1621 accatccggc aagagcagcg ggattgagag cacaccggcc agagtgatcg ccccacgcac
     1681 cccggcgaaa gacgcgatca ggatttctcg tgtggtccac gaaccaaact ccatcggctt
     1741 cttcttcagg aagcggttgc tgaacttttt catcgtccac agccagccga aacggaccag
     1801 catcagcgcc gcatatatca gaataatatc ggtaaacagc atccagattt cgacgttagg
     1861 gtcgatttct gctgccatca gcgacgtctc cagaataccc ggcagctgca gacctaacag
     1921 caggaacacc atgccgttaa agacaaattc cagcatcgcc caggtgctgt ttgcacgcag
     1981 gcgcattgcc agcggcgcac ggcgcatcac accggagcgg gtgatggtca tcccggcggc
     2041 aaccgcagcg aggatgccgg aaacgccaat atgttcggca atcagataag aagcgaatgg
     2101 cagcaagaac agcagaacaa tctgcgtcgc gggttcatca ccgccccagc ggctgaggaa
     2161 tcgcagcgag cgaccataca gccagctcac cacaaaaccg gcaagaatac cgccaatggc
     2221 tactttcata aattcgaccg tcgcaccgcc gacggtgaag atcatcgtcc ccatcgccac
     2281 tgccacggca aacttcaacg acaccaggcc ggaagcgtca ttcatcaacg cttcgccctg
     2341 caaaatgccc atgatttttt tcgggatgcg cccttcgcca acaatcccgg agagcgccac
     2401 agcatcggtc ggagaaagta ccgccgccag cgcaaaggcg gggatcagcg gaatgcccgg
     2461 caccacccag taaataagga agccaatgcc gaccacggtg accaccacca gcgccagcgc
     2521 gaggccgaaa atctctcgac catgttcgag aaattcacgg gtcggcgttt tccagccatc
     2581 agcgaacagc aacggcggga taaacaagac taaaaagagt tcaggatcaa actccacatg
     2641 caaaccaaac gtcggccacg ccagtagcgc accgatggcg atttgcataa gcggaagcgg
     2701 gatctgaaag ggcatgacac gagtgaccac cccggacagc gagaccacga gggtcattat
     2761 cagtatggtg aagaagattt ccatgcgttc cctgtttgcg atgtttttta tgatctgaga
     2821 aagacgttat gcctgaattc tcattttttc agaatatcgc aaaagctcac acaatgtgcg
     2881 gagaaaataa aaacgggcag ccattgccac ccgttttcag aatccgatga aagattagat
     2941 agcccagcca cccgcataga aggtcaccag cgccacggcg ataacgaccg taccgatgtt
     3001 caacttgcgc cattcgccgg aaaccagacg accaatcacc agagtcgcga agccgatcat
     3061 gatgcctgtt acgatgttac aggtcagcac gatgaatacc gccgtaacca gacccgccat
     3121 cgcatcaaca aaatcagcaa agtcgatttt cgccacgttg ctcagcatca gcaggccaac
     3181 gtacatcagc gccggagccg ttgcataccc cggaacgagg taagagagcg gagagaggaa
     3241 cagaatcagc aggaacagca cgccaacggt gatagccgtc aaaccggttt taccgcccgc
     3301 cgccgtaccc gccgcagact cgatgtatac cgctgccgga gctgcaccca ccaggccaga
     3361 gaaaacgctg ctcatggagt cagtggtcag tgctttccca ccgtcgataa tttgcccatc
     3421 tttatccagc aggttcgcct ggccggcgac ggcacggata gttccggtgg catcaaatac
     3481 tgccgtcatt accagcgcca gaacgcttgg caggactaca ggattcagcg cgcccataat
     3541 gtccaggctg ccaatcagtg agttgccgtt ttcatcgctc aatgaaggca tggcgaaaac
     3601 gccggagaaa tggacgttag gatcgaagat caaaccgaca attgagatac cgataatggt
     3661 cagcagaatg ccaccaggga ctttcagttt ttccaggccg atgatcaccg ccagacctac
     3721 cagtgacata atcaccggga aggtcgcgaa atcacccagc gcaacgggca gaccatcaag
     3781 cgggttttta atcaccagac cgacaccgtt agcggcaatg agcagcagga acagaccgat
     3841 accaatcccc gtgccgtgcg ccacaccgtg cggcaagttg cgcaaaatcc agctacggat
     3901 acccgtggca gaaattaccg taaacagcac acccatcagg aacacggcac ccagcgcgac
     3961 aggtacgcta atatgttgcc ccagcaccag gctgaatgcg gtaaacgccg tcagggagat
     4021 ggcgcaacca atcgccaacg gcagattagc ccacagaccc atcacgatag aaccgagtcc
     4081 ggcaaccaga caggttgcaa cgaaaactgc cgcaggcggg aagcctgctt tacccaacat
     4141 acctggaacg acgatgaccg agtagaccat cgccagaaac gttgttaacc cggcaaccac
     4201 ttcctgacgg acagtgcttc cacgttgtga aattttaaac caggcgtcga gtgaaccgcc
     4261 ggtacgcgct gatggcgtag acataagaaa acatcccctg agagtttaat tttcgtcaag
     4321 atgcgtggcg gacaatcccc tgccagcgaa acgttatctt cctgctccag gtttgcgaca
     4381 atttactgcg tcgcaaaaaa gcaaacgttt agctccgcgc atacaaaacg ggtggtaaaa
     4441 aaagcaaacg attatccggc gatcatagcg gctttttcaa ccgaagtttg ctgctttttt
     4501 ctcttttatt gtcttgacga tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcggt
     4561 gcgcaagatt gcagcgttat cgagccgtat accgttcagg attgcgataa aaaatcgtct
     4621 gagatgacaa aaatattgcg gataaaaagt atacttttac actggcgaag aaatgccggt
     4681 gacatacaat taaagcatca acaccaaccg gaacctccac cacgtgctcg aatggggtgt
     4741 gttgacgtcg ggggaaaccc tcctgtgtac cagcgggata gagagaaaga caaagaccgg
     4801 aaaacaaact aaagcgccct tgtggcgctt tagttttgtt catcttccag caagcgtgcg
     4861 ccggtacctt cttctcctaa gcggtcgccc gggttacgca acgggcaatc actgcgcgaa
     4921 aggcagccac aaccaataca tccgtccagt tcgtcacgca gcgctactaa ggtatgaatg
     4981 cgccgatcca actcttctcg ccattgggac gaaagctgtt tccactcttt cgcacttaac
     5041 gtatgccctt cgggcaacac gccaaacgct tcaccaatgg tcgccagcgg aatgccaata
     5101 cgctgagcaa ttttgataat tgcaacatat cgcaacacat cacgtttata tcgccgctga
     5161 ttgccgctgt tacggatact ggtaatcaac cctttacttt catagaaatg cagcgccgat
     5221 accgccacac cgctgcgttt cgccacttcg ccgggggtta gcagcgcttt aatgcggggt
     5281 aatttctttt ccataaatcg ctttacctca agttaacttg aggaattata ctccccaaca
     5341 gatgaattaa cgaactgaac actgaaaaga ggcagattta tgtcccatca gaaaattatt
     5401 caggatctta tcgcatggat tgacgagcat attgaccagc cgcttaacat tgatgtagtc
     5461 gcaaaaaaat caggctattc aaagtggtac ttgcaacgaa tgttccgcac ggtgacgcat
     5521 cagacgcttg gcgattacat tcgccaacgt cgcctgttac tggccgccgt tgagttgcgc
     5581 accaccgagc gtccgatttt tgatatcgca atggacctgg gttatgtctc gcagcagacc
     5641 ttctcccgcg ttttccgtcg gcagtttgat cgcactccca gcgattatcg ccaccgcctg
     5701 tagttttatt gcccgcgcgt taactcccgc gcgggtaatt gctccatcca ttgcataaac
     5761 acctgcggcg gcatcgcctt cgcaaaaaac catccctggc aatagcgcac gccgcgtttg
     5821 cgcagccagt taacctgctc ctcagtttcg acgccttcag cgatcgtttt taaccccagg
     5881 ctgtgcgcca gctcgatgat gtgttccgca atcaaatgac tggttttgtg ggtggtcagc
     5941 gtttcaacaa acgatttatc gattttcaaa atatcgacat tcaatgattt aaggttatgc
     6001 aagttagagt agccaatacc aaaatcatca attgccactt cgtaaccagc ctggcggaaa
     6061 gccagaataa tcggcgtcat tttttcgaca tcaagaaatg catgctcagt cacttcaaat
     6121 ttaatttgct gcggacgcac cgcgtattgc tctgttttct gattgattcg cgctatcaac
     6181 cgtgacgtat gaaaatcgga ggccgacagg ttaatagaaa catagcgatc ggcatgtgtt
     6241 gccaagtacg cgcccagatc gcggaagaca ttatcaataa cataatcagt tatctgttct
     6301 atcatcccct ccttttctgc cagcggaata aactctgccg gattcattat ttgcccctgc
     6361 tcaccaagcc aacgtaacaa cgcttcagcg ccgatacatt tttctgtttt gatatcgatt
     6421 attggctggt aataaagaca aagttgatgt ttttcgaggg cgcgttgcaa tttacgtttg
     6481 ggagataaat agttttgtcg aatacgtagc cagagtaata gcagaacaag actccccaaa
     6541 ataccggcgg gcaacgcaaa aataagatga ttataaaaat gggttataag acgttgatat
     6601 gaagtcgcaa caatggctgc aattgggcgt tttgtcgaat aaactgtcgc atataaatag
     6661 ccatttcttt gtacggttaa atccttcaaa tgaatcagcg gagaaaacgt tgctgccgag
     6721 gcctctttgc ttaacgagaa aaaggttttc gtcaccgtat catacacacc ccattgcaat
     6781 gtcgggtcat cagacatcac ttcactccag aagagagggt tgataaccgc cacataattt
     6841 ccccgctgca tataggtcat tttatagcca gagaaaaaag gcgtatcgcg gtaataatag
     6901 atagaaacgt taggttcacg cttataatcg gccggtgcaa tcgtatagcc gtttacaggc
     6961 gctatcagcg atgagcataa aaaatggtta tcacgggcat agatcaattc attaatataa
     7021 agatagccac gaataatatt caacattcgc ttttgatggg ctggagtgca aacttgcccc
     7081 tgatagcgtt cagctgcatc gctcactaaa tctgcctggc gaatgaccag ctcggattta
     7141 tccagtgcca gttgagcaaa ggttcggagc tgattattca cttctgattt tgcccaaagg
     7201 aaggcaatcc atagcgaaag aatgatggga aagagaacta aaaagataat gcccggcaac
     7261 gccagtaatt ggtgtcgtgc acgatgactc attgctatca atttcctgcc tgtttatacc
     7321 cggccattgc aaggtgaaaa ccctgggtac cgacgtcttt agataaatct tacggtataa
     7381 atagcacttt tgcctgattt gaaattgata cctttctaat cgtcacctgg atgaaaataa
     7441 ttcattgata ttcaaaatta atttcctaca ggaatgcaga ggcggcggga agatatgccc
     7501 ttaatgccac tgaaaaacca cataatctgt acagcaataa aagtcacggc ctaatatggc
     7561 taacaaacaa tcaattgtga tatagttcac aaaattaatg aaacaaacag agtgtttcat
     7621 ttttgtgttc tgcaagcccc gcgatagcca gagtgtacca cgcctcccgt gaacaacgcc
     7681 gcgctgtcca gggcatcggc tcttttcaga ggaattgttt gatggctacc ctcaccactg
     7741 gcgtggttct tcttcgctgg caacttctta gtgccgtaat gatgtttctg gccagcacac
     7801 tcaacatccg ttttcgtcgg tctgattatg tcgggcttgc ggtgatcagc agcggtctgg
     7861 gcgttgtttc cgcctgttgg ttcgcaatgg ggttgcttgg catcacaatg gcggacatca
     7921 ccgctatctg gcacaacatc gagtcggtga tgatagaaga gatgaatcag acaccgccac
     7981 aatggccaat gattttgacc tgatacgtag aagcctccat aacggaggct tcttttttat
     8041 ggctggcaga tgctttaatc atccacctta aaacaatata acctattgtt ttaatgacaa
     8101 atcagaacgg aatgtcatca tcaaagtcca tcggcggctc gttagacggc gctgccggag
     8161 cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccaccc tgcggctgct
     8221 gaggctgacc ccaaccgccc tgcggctgac caccaccgat attaccacct gccggagcgc
     8281 caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc acgacttctg
     8341 tggtgtagcg atcctggccg gattgatcgg tccatttgcg ggtacgcagc tgaccttcga
     8401 tataaacctg agaaccttta cgcagatatt cgctggccac ttctgccagt ttgccgaaca
     8461 gcacaacgcg gtgccattca gtctgttctt tcatctcgcc ggtcgcttta tcacgccagg
     8521 attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg tagcgtactt
     8581 ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg ctggccatgt
     8641 tcgtgtctcc tgaaaaaaat cgttctgaat aagtgtaaac gcgcgattgt accattacca
     8701 atagcgcttt tactatgttg tgacctcggt tccgggaaac aaacctggcc agacattgtt
     8761 acacaacact ccggataatg cattccaata ctgtatattc attcaggtca atttgtgtca
     8821 taattaaccg tttgtgatcg ccggtagcac catgccaccg ggcaaaaaag cgtttaatcc
     8881 gggaaaggtg aatggataag atcgaagttc ggggcgcccg cacccataat ctcaaaaaca
     8941 tcaacctcgt tatcccccgc gacaagctca ttgtcgtgac cgggctttcg ggttctggca
     9001 aatcctcgct cgctttcgac accttatatg ccgaagggca gcgccgttac gttgaatccc
     9061 tttccgccta cgcgcggcag tttctgtcac tgatggaaaa gccggacgtc gatcatattg
     9121 aggggctttc tcctgccatc tcaattgagc agaaatcgac gtctcataac ccgcgttcta
     9181 cggtggggac aatcaccgaa atccacgact atttgcgttt gttgttcgcc cgcgtcggcg
     9241 aaccgcgctg tccggaccac gacgtcccgc tggcggcgca aaccgtcagc cagatggtgg
     9301 ataacgtgct gtcgcagccg gaaggcaagc gtctgatgct actcgcgcca atcattaaag
     9361 agcgcaaagg cgaacacacc aaaacgctgg agaacctggc aagccagggt tacatccgtg
     9421 ctcgtattga tggcgaagtc tgcgatcttt ccgatccgcc gaaactggaa ctgcaaaaga
     9481 aacacactat tgaagtggtg gttgatcgct tcaaggtgcg tgacgatctt acccaacgtc
     9541 ttgcggagtc gtttgaaacc gcgctggagc tttccggtgg tacagcggta gtggcggata
     9601 tggacgaccc gaaagcggaa gagctgctgt tctccgccaa cttcgcctgc ccaatttgcg
     9661 gctacagtat gcgtgaactg gagccgcgac tgttttcgtt taacaacccg gcaggtgcct
     9721 gcccaacctg tgacggcctt ggcgtacagc aatatttcga tcctgaccgc gtgatccaaa
     9781 accccgagct gtcactggct ggcggtgcga tccgtggctg ggatcgccgc aacttctatt
     9841 acttccagat gctgaaatcg ctggcagatc actataagtt cgacgtcgaa gcgccgtggg
     9901 gcagcctgag cgcgaacgtg cataaagtgg tgttgtacgg ttctggcaaa gaaaacattg
     9961 aattcaaata catgaacgat cgtggcgata cctccatccg tcgtcatccg ttcgaaggcg
    10021 tgctgcacaa tatggagcgc cgttataaag agacagaatc cagtgcggta cgtgaagaat
    10081 tagccaagtt tatcagcaat cgcccatgcg ccagctgcga agggacgcgt ctgcgtcggg
    10141 aagcgcgcca cgtttatgtc gagaatacgc cgctgcctgc tatctccgac atgagcatcg
    10201 gtcatgcgat ggaattcttc aacaatctca aactagctgg tcagcgggcg aagattgcag
    10261 aaaaaatcct taaagagatc ggcgatcgtc tgaaattcct cgttaacgtc ggcctgaatt
    10321 acctgacact ttcccgctcg gcagaaacac tttccggcgg tgaagcccag cgtatccgtc
    10381 tggcgagcca gattggtgcg ggcctggttg gcgttatgta tgtactggac gagccgtcta
    10441 tcggcctgca ccagcgcgat aacgagcgcc tgttgggtac gcttatccat ctgcgcgatc
    10501 tcggtaatac cgtgattgtg gtggagcatg acgaagacgc aattcgcgcc gctgaccatg
    10561 tgatcgatat cggtcctggt gcgggtgttc acggcggtga agtggtcgcg gaaggtccac
    10621 tggaagcgat tatggcggtg ccggagtcgt taaccggaca gtacatgagc ggtaaacgca
    10681 agattgaagt gccgaagaaa cgcgttccgg caaatccgga aaaagtgctg aagctgacag
    10741 gcgcacgcgg caacaacctg aaggacgtga cgctcacgct gccagtcggt ctgtttacct
    10801 gcatcacagg ggtttcaggt tccggtaaat cgacgctgat taacgacaca ctgttcccga
    10861 ttgcccaacg ccagttgaat ggtgcgacca tcgccgaacc ggcaccgtat cgcgatattc
    10921 aggggctgga gcatttcgat aaagtgatcg atatcgacca aagcccaatt ggtcgtactc
    10981 cacgttctaa cccggcgacc tataccggcg tgtttacgcc tgtgcgcgaa ctgtttgcgg
    11041 gcgtaccgga atcccgtgcg cgtggttata cgccaggacg tttcagcttt aacgtccgtg
    11101 gcgggcgctg cgaagcctgt cagggcgacg gtgtgatcaa agtagagatg cacttcctgc
    11161 cggatatcta cgtgccgtgc gatcagtgta aaggtaaacg ctataaccgt gaaacgctgg
    11221 aaattaagta caaaggcaaa accatccacg aagtgctgga tatgaccatc gaagaggcgc
    11281 gtgagttctt tgatgccgta cctgcactgg cgcgtaagct gcaaacgttg atggacgttg
    11341 gcctgacgta cattcgcctg gggcagtccg caaccacact ttctggtggt gaagcccagc
    11401 gcgtgaagct ggcgcgtgag ctgtcaaaac gcggcaccgg gcagacgctg tatattctcg
    11461 acgagccgac caccggtctg cacttcgccg atattcagca actgctcgac gtactgcata
    11521 aactgcgcga tcagggcaac accattgtgg tgattgagca caatctcgac gtgatcaaaa
    11581 ccgctgactg gattgtcgac ctgggaccag aaggcggcag tggcggcggc gaaatcctcg
    11641 tctccggtac gccagaaacc gtcgcggagt gcgaagcatc acacacggca cgcttcctca
    11701 agccgatgct gtaatcgtta aggccgcttt ctgagcggcc ttttcctttc agagttgtac
    11761 cagcaattta cgcttttctt ccggtagtaa attcactgcc tgctgataag acgcatccac
    11821 cagataatag atttgcgaat ccggcagcga accgtcgaga tacacggtgc tccagtgcgc
    11881 tttattcaga tggcggctag gacgcacatc gctgtgctgc tgacgtagca gctccgccag
    11941 ctccgggctg gttttcagcg aaacagctgg gcgattttct acttctttca ccatcgcaaa
    12001 cagtacatct tccactttga tctgcgtcgc tttccagtcg ttatgcacgc tctgttctgc
    12061 gcctggtttt gccatgcaat attgtagcaa ctccgaaatg gtcatttttt actccccttg
    12121 tagtgtcgcg atgatgcgac gcgatccgcc gtggatgcga tgttccccca gccagatgcc
    12181 ttgccaggtg ccggtctgaa tacgcccttt atgtaccggc aatacaagcg atgttcccag
    12241 cattgaggat ttgatatgag aaggcatatc gtctgctccc tcatagtcat gctcataatt
    12301 tccgttgtcg ggaacggtgc ggaggaaaaa acgctccatg tcgtggcgta cggtgggatc
    12361 gcagttctca ttaagtgtca gagaggcgga ggtatgttgc agcaacagat gcagtaagcc
    12421 gatgttaacg cgcggcatat cagccagctg attcagaatt tcatccgtta ccagatgaaa
    12481 cccacgagat ttggcgctaa gcgtgagcgt cttttgatac cacatgtgct gctccttgat
    12541 aaaactctct taatcagttt gcagcaagac agcgaaagga taaaggtgtg attaaaaaaa
    12601 cagcattcgg gagagcgtca cgctctcccg ctctgtcagt attctgaatt gacgatcacc
    12661 tcttcaccaa acgcacccgc ttgtggcaag ggtttgtagg tagagttgga ggcgcgcaga
    12721 atgcggatac cacgagcgcc gacatcgcgt gcggcggtaa tatcattatc agaatcgcca
    12781 taaaaaattc ggatattttt atcctgcagc cattgcgatt ttgtattttg ccctggttta
    12841 tcgcccgcaa agatcaccgg attcatgttg gtggcaggaa tatgaaaatt atccgccagc
    12901 gtttttgaaa ccgtttctgt tttcgtcgga ctacgaccag tcacaaagaa gatcgcgtca
    12961 ccgcggcgta catgcatatc aatcagctgg cgagcgacct cttttggaat gctgaattca
    13021 tcccagccat tgttcatttt ttcccagaac acaggatttt tcagataatc ttcgctttct
    13081 ggcgagaagg tttttttgcc gcgccagaag cccggactgg aaaaaagtac cgtgtcatcg
    13141 atatcaaacc ccaccgccat tggcggacgc cctgcgaggc tattttcaat ttgtgcgacc
    13201 gaaacccaat gaatgggtgc ctgttcagca agcctggcaa cgttagtccc agggttaagc
    13261 ggtgaaggag atgaggccag ggcaacagcg gaactgttta gagcaaacaa taagcaaacg
    13321 gcactgattg cctgtgtgat cttgcgcata tttttcccta aatagtcagt atgttgaaac
    13381 tttttattgt gagatttgtt gcaaaaacca tctgaccata acgccagctg ccggggaaag
    13441 gaaggattat ctgcggtttt tgtgatgaaa agcaggatga ttatatttaa cccagtaaat
    13501 acgttcctgt ctttttttgt ataaaaaatg tttattactc gatgtaagga tacattaatt
    13561 ttacgtttat tatgatgaat cctcggcaaa tgagcgaaat ttgatagaac atttatctgc
    13621 tgtacgattt aatgaactta atgatacagc attacaaaaa caatcgaagt ttataaagat
    13681 gatt
//
LOCUS       DAZFYC010000130        13474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFYC010000130 DAZFYC010000000
VERSION     DAZFYC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.130"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(123..199)
                     /locus_tag="ACHKW5_004211"
     tRNA            complement(123..199)
                     /locus_tag="ACHKW5_004211"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Met,seq:cat)
     gene            408..1505
                     /gene="mltA"
                     /locus_tag="ACHKW5_004212"
     CDS             408..1505
                     /gene="mltA"
                     /locus_tag="ACHKW5_004212"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO7513470.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1582..2388
                     /gene="tcdA"
                     /locus_tag="ACHKW5_004213"
     CDS             1582..2388
                     /gene="tcdA"
                     /locus_tag="ACHKW5_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO7513471.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2439..2882)
                     /gene="csdE"
                     /locus_tag="ACHKW5_004214"
     CDS             complement(2439..2882)
                     /gene="csdE"
                     /locus_tag="ACHKW5_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO7513472.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2882..4087)
                     /gene="csdA"
                     /locus_tag="ACHKW5_004215"
     CDS             complement(2882..4087)
                     /gene="csdA"
                     /locus_tag="ACHKW5_004215"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO7513473.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4279..4506
                     /gene="ygdI"
                     /locus_tag="ACHKW5_004216"
     CDS             4279..4506
                     /gene="ygdI"
                     /locus_tag="ACHKW5_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO7513474.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4857..5774
                     /gene="gcvA"
                     /locus_tag="ACHKW5_004217"
     CDS             4857..5774
                     /gene="gcvA"
                     /locus_tag="ACHKW5_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO7513475.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5793..6188
                     /gene="ygdD"
                     /locus_tag="ACHKW5_004218"
     CDS             5793..6188
                     /gene="ygdD"
                     /locus_tag="ACHKW5_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO7513476.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6181..7281
                     /gene="rlmM"
                     /locus_tag="ACHKW5_004219"
     CDS             6181..7281
                     /gene="rlmM"
                     /locus_tag="ACHKW5_004219"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO7513477.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7325..8056)
                     /gene="fucR"
                     /locus_tag="ACHKW5_004220"
     CDS             complement(7325..8056)
                     /gene="fucR"
                     /locus_tag="ACHKW5_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO7513478.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8114..8536)
                     /gene="fucU"
                     /locus_tag="ACHKW5_004221"
     CDS             complement(8114..8536)
                     /gene="fucU"
                     /locus_tag="ACHKW5_004221"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO7513479.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8538..9956)
                     /gene="fucK"
                     /locus_tag="ACHKW5_004222"
     CDS             complement(8538..9956)
                     /gene="fucK"
                     /locus_tag="ACHKW5_004222"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO7513480.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10065..11840)
                     /gene="fucI"
                     /locus_tag="ACHKW5_004223"
     CDS             complement(10065..11840)
                     /gene="fucI"
                     /locus_tag="ACHKW5_004223"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO7513481.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11873..13189)
                     /gene="fucP"
                     /locus_tag="ACHKW5_004224"
     CDS             complement(11873..13189)
                     /gene="fucP"
                     /locus_tag="ACHKW5_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7513482.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3278 a         3338 c         3541 g         3317 t
ORIGIN      
        1 tgattggggg ggatcaacgg tttactcatt gtttcaggct tcactgaaac ggataaggaa
       61 gcgtaaaaac gaaaaaaccg caagctaaag gtagcgttgc ggtttctgat attctaataa
      121 attggttgcg ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac
      181 caggctgctc caccccgcgt ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa
      241 cctttttttt accccgatca cggatttagc atgattttga acaaaaacaa accactaaag
      301 cctatttttt gtacaaaatt tgcgtcaggg agaggctgct catttcatta aacgcggcgg
      361 tttgttatct tcgttgcgcc ttatttttta acctgaagaa gagaacaatg aaaggacgtt
      421 gggtaaagta ccttcttatg ggcacggttg tggcaatgct tgccgcctgc tcttccaaac
      481 caaccgatcg cggacagcaa tataaagacg ggaaatttac ccagcctttc tctctggtga
      541 accagccaga tgccgttggc gcgccgatta acgccggtga ttttgccgag caaattaacc
      601 atatccgtaa ttcgtcaccg cgtctgtatg gcaaccagag taatgtttat aacgcggtgc
      661 aagagtggct gcgcgcaggc ggtgataccc gcaatatgcg ccagttcggc attgatgcct
      721 ggcagatgga aggtgccgac aactatggta acgtgcagtt tacaggctat tacacgccgg
      781 taattcaggc gcgccatacc cgccagggcg agttccagta tcctatttac cgtatgccgc
      841 caaaacgtgg tcgtctgccg tctcgtgcgg agatctacgc aggggcgctg agtgataaat
      901 atattctcgc ttacagcaac tccctgatgg ataacttcat tatggatgtg cagggtagcg
      961 ggtatatcga ctttggtgat ggcagtccgc ttaacttttt cagctatgca gggaaaaacg
     1021 gtcatgccta tcgcagcatt ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag
     1081 atatgtcgat gcaggcgatt cgtcattggg gcgaaacaca cagtgaagcc gaggttcgcg
     1141 agctgctgga acagaacccg tctttcgttt tctttaaacc gcaatctttt gcaccggtga
     1201 aaggggcaag tgcggtgccg ctggttggcc gtgcttctgt tgcctccgat cgttcaatta
     1261 ttccgccagg tactaccttg ctggcagaag tgccgctgct ggataataac ggcaaattta
     1321 acggtcagta cgaattgcgt ctgatggtgg cgctggatgt cggtggcgca atcaaaggcc
     1381 aacacttcga tatctatcaa gggatcgggc cggaagccgg acatcgcgca ggttggtaca
     1441 accactatgg acgtgtctgg gtgctgaaaa ccgccccggg cgcaggtaac gtctttagcg
     1501 gctgatgtgt tattctgtat accgttttta cggatacgcc agggtgaggg ataacctcac
     1561 cctttttaat tctgaggtgc tatgtctgtg gtaattagtg atgcatggcg tcagcgtttt
     1621 ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac tgtttgctga cgcgcatatt
     1681 tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg aagcgctggc gcgcacgggg
     1741 attggcgcaa tcacgcttat cgatatggat gatgtgtgcg tcaccaatac caatcggcaa
     1801 attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg aagtcatggc ggagcgtatt
     1861 cgccagatta acccggagtg ccgtgtaacg gtggtggatg atttcgtgac gccggataac
     1921 gtagcgcagt atatgagcgt gggttattcg tacgtgattg atgccattga tagtgtacgg
     1981 cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa tcccgctggt cacgaccggt
     2041 ggcgcgggtg ggcagattga cccgacgcag attcaggtta ccgatctggc gaaaacgatt
     2101 caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa gcgattttgg cgtagtgaaa
     2161 aacagtaaag gtaagctcgg cgtcgattgc gtgttttcta ctgaagcgct ggtgtacccg
     2221 cagtcagacg gtactgtgtg tgcgatgaaa gccacggcag aagggccgaa gcggatggat
     2281 tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca cctttgggtt tgttgcggtt
     2341 tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc agggttaaac aatcaggtcg
     2401 gatgcgacgc gaacgtctta tccgacctgg ttctgtgctt aaacctgctt cgccgcagcg
     2461 ataatcgcct cgcttaacgc attcaacccc tggctgcgtg aggcgctaag ctgtgcacgc
     2521 aatcccagct catcaaacaa tgccagcggc gactgtgcct gcaattcggc gacggttttc
     2581 ccctcaacgg cagtcaacaa caccgccaac aggccgcgca caatgcgccc ttcgctgtcg
     2641 ccaaagaagt gcattttacc gttttcagtc acggtatatc ccagccagac gcggttttcg
     2701 catccggcaa tctctttagc ctgcgctttt aactcgtctg gcaaagccgg aagctgtttc
     2761 cccagcatga tcaactggcg atatttatct tcccattgcg acaacggtgc gaaggtattg
     2821 cgtaacgttt ctgcggttac ggttgtgccg aacggatgtc cggcgaattg cgggtttgtc
     2881 attaatccac caataattcc agcgcgcggt caacggcatt caccagcgca tccacatcac
     2941 tctttgtatt atatggcgca aaagaggcgc gcagtgtgcc ggttacgcct aattctgcca
     3001 gtagcggctg agcgcaatgc tgcccggccc gcagggcaat accgtactcc gccagcagcg
     3061 tcaccatatc gctatgatga acgccagcaa aatcaaaggc cagcaggctg gaatcctggc
     3121 agcggaatga acgaaagccg ggacgtttcg ccagcgcatc ttccgccagc gttgctaagc
     3181 tacggctcca gctttcggcc tggttgatat cgtaatctgc cagccattcc agcgccgcgc
     3241 ttaatcctat gacaccagcg acatttggcg ttccagcttc cagtttccac ggcgcagatt
     3301 gagtcgtgaa gccgtcaaaa ctcacttcgt gaatcatttt gccgccaccc agccagggcg
     3361 acatcgcttc cagcagttct gatttgccat acagcacgcc gatacccgtc gggccataca
     3421 gtttgtgacc agagaaagca tagaaatcga tatctagttg ctgaacatct gcggggaaat
     3481 gcaccgcccc ctgagcacca tcaaccatca ccaccatccc ggctgaatga gcaaaggtaa
     3541 tcgctcgcgc cagatccggg caaccgccag taacgttcga catctgaccc aacgccagaa
     3601 tccgactacg gggagtaatc agttctggca acaaatcgac atccggcagt cgctgcgcat
     3661 taagcggcaa tttcaccact ttggctccag tttgttgggc gaccatcagc caggggacga
     3721 ggttagcgtg gtgttctgcc acgctgacaa taatctcatc gcccggttgc agacgcggac
     3781 gcgcatagca ttgtgccacc atgttgatgg attcagtggt gccgcgcgtc cagacgatag
     3841 ttttatcatc cggtgcattc agtaattgcg ccactttctc tcgtgcagct tcataacgcg
     3901 cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac gtttccggcg ctcaggctgt
     3961 aaaactgtcg ggtggcttca accacggctt caggtttaag cgcggtcgcg gcgctgtcga
     4021 gatagacgcc cgcatcctgt agagcgggaa actgggcgcg aaactgcgcg ggattaaaaa
     4081 cgttcatggt actcctcggc ttgatgcacc gatcgtgacg caaaattcac tttgatacaa
     4141 ggagagtgct aaccatcaga attatctgga tatcctggcg aagccgcgta agtaacatat
     4201 gctgaatagt gttaggcgtt aattttagcc tgttaatcaa tactattaag atttattaat
     4261 cgctttaagg agaagatgat gaaaaagact gccgcaatta tttctgcctg tatgctgact
     4321 tttgccctga gcgcctgttc cggttcgaac tatgtgatgc acaccaatga cggacgtacc
     4381 atcgtctctg acggcaaacc acagactgat aacgataccg gtatgatttc gtataaagac
     4441 gctaatggca acaaacagca gatcaaccgt actgacgtga aagagatggt cgaactggat
     4501 cagtaattca cgatcgcaag gtaaaaaaaa gcaccgcaat taggcggtgc tacattaatc
     4561 actatggaca gacagggtaa atgtacagga agtgaaaaaa ggtagctttg ctaccatggt
     4621 ctgaatcgca gaccaattgc aaacacaaca acacaacatc acaaccgtaa gccaaaagtt
     4681 caccagaaca cgcattccga taaaactttt cgttccggct caggaagtgc cgccactata
     4741 ggtatttgct ggtagaagct caacggacaa tttataatgg ctcagattaa aaaaactaat
     4801 aggttacaca gtgtgatata attgttaaat tcatttaaca tcaaagttta aaagccatgt
     4861 ctaaacgatt accaccgcta aatgccttac gagtttttga tgccgcagca cgccatttaa
     4921 gtttcactcg cgcagcagaa gagctttttg tgacccaggc cgcagtaagt catcaaatca
     4981 agtctcttga ggattttttg gggctaaaac tgttccgccg ccgtaatcgt tcactcctgc
     5041 tgaccgagga agggcaaagc tatttcctcg atatcaaaga gatattttcg caattaaccg
     5101 aagcgacgcg taaactccag gcccgtagcg ccaagggggc gttgacggtc agtttactcc
     5161 ccagtttcgc cattcattgg ttggttccgc gactttccag ctttaattca gcttatccgg
     5221 gaattgacgt tcgaatccag gcggttgatc gtcaggaaga taagctggcg gatgatgttg
     5281 atgtggcgat attttatggt cggggcaact ggccggggct acgggtggaa aaactgtacg
     5341 ccgaatattt attgccggtg tgttcgccgc tactgctgac aggcgaaaaa cccttgaaga
     5401 ccccggaaga tctggctaaa catacgttat tacatgatgc gtcacgccgt gactggcaga
     5461 catatacccg acagttgggg ttaaatcata tcaacgttca gcaagggcca atttttagtc
     5521 atagcgccat ggtgctgcaa gcggctattc acgggcaggg agtggcgctg gcaaataacg
     5581 tgatggcgca atctgaaatt gaagccggac gtcttgtttg cccgtttaat gatgttctgg
     5641 tcagtaaaaa cgctttttat ctggtttgtc atgacagcca ggcagaactg ggtaaaatag
     5701 ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga acaagaaaaa ttccgctttc
     5761 gttatgaaca ataatttacg tagggtacga ccatgaccag ccgttttatg ctgattttcg
     5821 ccgccattag cggcttcatt tttgtggctc tgggcgcttt tggcgcgcat gtgttaagta
     5881 aaaccatggg ggccgttgag atgggctgga tccagaccgg cctcgaatac caggcgtttc
     5941 atacgctggc gatcttaggt ctggcggtgg caatgcagcg tcgcatcagt atctggtttt
     6001 actggagtag cgttttcctc gcgttaggca cggtgctgtt cagcggcagc ctttattgcc
     6061 tggcactgtc ccatctgcgt ttgtgggcgt ttgtcactcc ggttggcggc gtgagcttcc
     6121 ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt aaagcgcaag ggcgtaagtc
     6181 atgaataagg ttgtattgct gtgccgtccg ggctttgaaa aagagtgcgc cgcagaaatt
     6241 accgataaag ccggccagcg ggaaattttc ggttttgccc gcgtgaaaga gaatgcgggt
     6301 tatgtcattt atgaatgtta tcaacctgat gatggcgata agttaatccg tgagctgccg
     6361 ttcagttcat taatttttgc ccgccagtgg tttgtggtgg gggaactcct gcagcatttg
     6421 ccgccagaag atcgtattac ccccattgtc ggcatgttac agggcgtagt agagaagggc
     6481 ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca aagagttgct gaaattctgc
     6541 cgtaaattca ccgtgccgct acgcgctgcc ttgcgcgatg ccggggtgct ggcgaactat
     6601 gaaacgccga agcgtccggt tgtgcatgta ttcttcattg caccaggttg ctgctatacc
     6661 ggttactcat acagcaacaa taattcgccg ttctatatgg gcattccgcg cctgaaattt
     6721 ccggcagatg cgccgagtcg ttccacactc aaactggaag aggcatttca tgtgtttatt
     6781 cctgcggatg agtgggatga acgcctggcg aacgggatgt gggcggtgga tttgggcgct
     6841 tgcccaggcg gctggaccta ccaactggtg aagcgcaaca tgtgggttta ttccgtcgac
     6901 aacggcccga tggcgcaaag tctgatggat accgggcagg tgacgtggct gcgggaagac
     6961 ggtttcaaat tccgtccgac acgcagcaat atctcctgga tggtatgcga tatggttgaa
     7021 aaaccggcta aagttgcggc attgatggcg cagtggctgg ttaatggctg gtgccgtgaa
     7081 accattttca atctcaaact gccgatgaaa aaacgctacg aagaagtatc acacaatctg
     7141 gcgtatattc aggcacagct tgatgaacat ggcataaatg ctcagattca ggcgcggcag
     7201 ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc gcatctgggc tgcggtgggt
     7261 ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc gtctccggcc tgctaccctt
     7321 tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc atcagaaata atgtgcgtta
     7381 cctcatccag atgcccgatg cgggcttccc ctgaacgatt aaatttactt ttatcaatca
     7441 ataacaacga ttgcgcggca cgttttaata gcatcgattt gtaatcagcg ttgatggcat
     7501 tggagtccca cagtgcgccg ctgctatcga tcccttcaca agaaaaaata aacagatcga
     7561 tttccagcga tttaagttgg gaaatcagcg agggattgac gtaacagcca tatttgcgct
     7621 caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg tttaccgagt tcatggcaaa
     7681 tcggatggct attggtgaag acctgaatgt tgatgtcagg caactggcgt gccagatacc
     7741 agcaagttga actggcgtct aaggctatca ccatcccttc ttcaatccac gcgagcgcct
     7801 cgcgcgcgat atctgcttta tgcgcataat ggcttttcag ccggatgtga aagggatcgc
     7861 cactgtcttg attttgacgg tggatatatt tagcgcgtcc atgattgcgc agaattttac
     7921 cctgcgtctg taattcattg agatcgcgac gaatggtttc tttactgacc tttagctgtt
     7981 cagagagagc ttccgtggtc aggctggtat ggttcagcag caggtcgact atcgcttgct
     8041 ggcgtgccgc tttcatattt ttcaccccat cattcgataa ccgcccgcgt catgcgggcg
     8101 taccggtatg agattacggt gttacccctt tttttaaaag aatattcccg tacttcgctc
     8161 gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg ttcataaaac gcaaaacgat
     8221 tgatgcggat gatgtcagga cacggggctt gtagtgaaag cgcattacgg taacgtcgtt
     8281 ctacttcagg atcgagagtg tcaccttcta ccgctgccat catcaccagc ggcggggcat
     8341 aactgtccag ctcaaataac gggataatcg cctggagcaa gtcgctcacc aacaggccat
     8401 cagcgcggat cacctgcggt cccatcgaat gggcgggaaa gtgagcatcg gaaaaaataa
     8461 tttcatctcc atgtcccatc tctgccagca ctttcaatag ttcgggagaa attaacggcg
     8521 aaattgtttt cagcatttca cacttcctct ataaattcag gttcagtttg cgggtagaaa
     8581 taacggtact gataatgaat ctgtgcacgg gcttcttccg ggctgttgaa ttcccctacg
     8641 ccataccaac cgaacagcgc agctcctgcg acggtcgttt cggcatcgtc gagaactttt
     8701 accggaatat caagcatatt ggctttaatc tgattccaca atgtgttgcg acttcctcca
     8761 ccgactaaca ataattcaga ggccttaaag tgcccgattt tttccagcat ctgtagattg
     8821 cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat agaaatgccc ccgcgtggta
     8881 ttaagcgtca ctccttgcca gccagcgttc tgacacgaca ataaatcaca ctgcattttt
     8941 acgccatccg cgccaggcgc gatcagacga gcttcttcaa tcaacatttg ccagggtgtt
     9001 tcagccgtcc agaacagttt tctcacccat tccagcacgc cggatgccag ccattgcata
     9061 cctgggttat acaaccctgc ctggctatcc agttcgcagg tggaaccggc gtactgactt
     9121 aacagcgaag tatcaacctg ggcgctacgg accattaaaa tttcccatgt accggaagag
     9181 agcacgggtt cattttggtc ggcaccagcg ccaaaaaggg cgaactgggt atcgtgacct
     9241 gcggaaatca ccggtatgcc aacgggtaag ccgagcattg ctgcggcgct gttctgtagc
     9301 gtaccaatct gttcacccgc ttccaccaga cgagggaaga gtcggcgtgg aataccggtg
     9361 gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat ccagcatctg gctggttccg
     9421 gccatcgtga tatcagtagt gaattcgccg gttaaacggt ggttaatcag cgacgaaata
     9481 aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat tttctttcaa ccacaccaac
     9541 ttatataacg tattgaaact aaaggctccg acgccagaaa tagcctgcaa ccgctgtgcg
     9601 gagatcaact gttcaatatt gtccataacc gctgctgttc gcggacattt ccagctaatg
     9661 atcggataga gcagattgcc ttgcttatct accagagcgc catccacacc aaaggtggtg
     9721 acggcgatac cgcggatgtg gcattcagtc agttcactat tgatttgccg acagcaatca
     9781 gcaaagcgtt gcaaaatggc gtctaaagac cactggtgcc aggtgttgtt ttccatcgcg
     9841 atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt ttccctgccg attaaccgcg
     9901 atggccctga cattggtcgc gccacagtcg agtaccagga taacttcttg tttcataatc
     9961 gctcctgcaa tatagccgga taacattgct tatccggcta accactcttg cttgttcagg
    10021 cccggtagcc ggagcgaccg ggcatcacat cagggagtaa tgtattaacg cttgtacaac
    10081 gggccgtagt tctggcaagc gcggtaatcc tggccttcaa tatccatgcc gtgcgcagcc
    10141 caggcagaag gacgatacac tttggtctct tcaacgttgt gcatacatac cgggatacgc
    10201 agcatggagg cgagagtgat aaagtctgcg ccaacgtggc cgatggtcag aaccccatgg
    10261 ttagcgcccc agttcgccat taccgagtac acatccgtaa acggcccttt accggtgagg
    10321 cgcggtgcaa accaggtggt tggccaggtt gagttggtgc gtttgttgag gatgtcatgc
    10381 acatccttcg gcaattccac gctccagcct tccgcgattt gcagtaccgg tcccaggcct
    10441 ttgatgatgt tgacacgggt catggtgaac gggacgccgc cttcggtaag gaagcgggaa
    10501 gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc accattcggt agcggcgagg
    10561 caagcgtcag cctcttgctg agagatttcc cagtgcggct tcatcgtcgg gttaccttcg
    10621 ctgtcgcgtt gtttacagga accgtccagc gcagcagaac cggagttgat caaatggatg
    10681 atgccgtgtt ctgccagtcc atcgagttta tgccccgtta cacgctcaat tgcttctggt
    10741 gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc cggtgagctg gtgacccatt
    10801 agcattgcca cgccgttaag actgtcgttt tcggtcgcca cgacaaaggg ttcgcgcacg
    10861 ccattccagt caaatgaact gttaaggatc gcttcggcgg tgtcaccatt gggatattga
    10921 tcggtccagt gacgttgccc ctggaagccc gcagcgatgg cgttgtagcc aagtgattct
    10981 tccacgcgac caatatcggc cagtttgctg ttgccttgca tcatgtcgcg gatacacatc
    11041 gccatcagta aactttcgcg cagaactgcg cggctttgct cggcattgcg ttgatactgt
    11101 ttgttatttt catcttcgcc atagcggaag tttttatcag cccaggtcag tgccatttcc
    11161 agttcggctt cgtcgtaaat cttctgatcg atacggcgac gcagttcggt catatccacc
    11221 gcctggactt tcattcccag ccaggattca aagaagttgt gatcaacaat agaaccggcg
    11281 atacccatcg aaacgccgcc cagcgacaga tagcttttac ctttcatgct ggcgacggcc
    11341 aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat cggcaggaat cgatgtgtca
    11401 tcggcatcct gaacgtcatg accgtaaatg gagaatgccg ggatgccttt ctggctgtga
    11461 gctgccagag ccgctgccag gtaaacagcg ccgggacgtt cagtgccgtt aaagccccaa
    11521 atggctttcg ggcgggttgg atccatgtcg atggtttcac tgccatagca ccagcaaggc
    11581 gttaccgtaa tggtgaggcc tacattctga ctgctgaatt tttcttcgca agcagcggct
    11641 tcagccatac ccgcgataca ggtatcggaa atgacacact cgacggcagc tccgcaggca
    11701 tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg ccatattcat tgtttgttct
    11761 tcaagcgact cacgaacacc catgcgacga ccgtcaataa ccgggcggat accaattttc
    11821 ggtaagctga tttttttcat tacagattcc tcactttatt cggaaaatat gttcagttag
    11881 ttgccgtttg agagcggaaa cgggcaaaga taaagatgac cgcgaagcag agtgcgggga
    11941 tcagttcagc agtggggatg ttgcccgccg cgtcactgac aaaacccatg accggagtga
    12001 caataccgcc gccaatgatg gtcataacga tgaaggacga accgtatttg gtgtcctggc
    12061 cgagattctt aatgcccagc gagaagattg ttgggtactg aatcgacata aaggcgctgc
    12121 ataaagtcag ggctattaag cccacatgac cgccagcgaa ggctgagatc aggcacagtg
    12181 ccatagcgat taatgcgtag gcggccagga ctttgtgtgg tgcgaagcga ctgatgagcc
    12241 aggtaccggt gaaacgacca ataaagaagc acaccatggt tccggttaaa tagttagcgg
    12301 caaagcctgc agtcatacct ggaatttctt ctacagcgta gcgaatcaaa tagctccagc
    12361 aggccgtttg tgcgccaacg tagcagaatt gcgctaatac cgcccagcgc cagtgacgaa
    12421 tacgcgccag acgagaaagc gatgcggaga acgatccttg tttggcgtca ctgtgattat
    12481 cactctgcaa tgccgggaat ttcgtcagca tgatcagcag ggcgaccagt aacacgatag
    12541 ccacgatgat catataaggt gtctgtaccg ataataccag gctgtgttta tacgcactca
    12601 attgctctgg agacatttta tcgagaacgt cttgcgattg atgtggcacg ttagacaaaa
    12661 taagactttg cccaaagaca accgcgataa ttgcgccaaa cgagttaaat gtttgcgcaa
    12721 gatttaagcg gaagtgacca ctgctttccg gccctaatac cgtaacaaaa gggtttgcgg
    12781 cagtttccag acaacctaat ccggctgcaa taataaatag gccaactaaa aataaggtgt
    12841 agttcattat ttctgcagcg ggccagaata atgcagcgcc caaggcatat aaaaataatc
    12901 cggtaataat ccctgcttta taactgagtt ttttcatcaa tatcccagca gggattggga
    12961 taatgaaata accaaagtaa aaggccgatt ggatcaggcc agcctggaaa tttgtcagcg
    13021 taaaagcctg ctggaattga ggtaataaaa tgtcgttaag gttattggct accgcccaaa
    13081 gaaaaaacag tgagcacagc agcgcgaatg gaataatgta acttctgctt tgccctgcat
    13141 ctttatctac cgcacggtaa ctctgcgttt gtattgatgt gtttcccata gcatcctctt
    13201 aggttcagaa gcttaagcac gtaatcggtt atgttttcaa aagtgcaaaa ccagcgagtt
    13261 cgggcttcat atcagtgaat atttgcctgt ctgaaatgaa ctcatggatt cattttcaaa
    13321 taaaaaacgc tctgtaatat gacggcggtc acacttatta gctggaaggt gactttatgt
    13381 gactaccatc actttaataa tcgaaatttc atgcccgttt cagaaaataa atcataaaaa
    13441 acacaaacca gatgaccgta ttaattgttt atgc
//
LOCUS       DAZFYC010000131        13371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFYC010000131 DAZFYC010000000
VERSION     DAZFYC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.131"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(2..193)
                     /locus_tag="ACHKW5_004225"
     CDS             complement(2..193)
                     /locus_tag="ACHKW5_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513483.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNK"
     gene            complement(190..612)
                     /locus_tag="ACHKW5_004226"
     CDS             complement(190..612)
                     /locus_tag="ACHKW5_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7513484.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAIVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(659..1084)
                     /locus_tag="ACHKW5_004227"
     CDS             complement(659..1084)
                     /locus_tag="ACHKW5_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7513485.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1496..2266)
                     /locus_tag="ACHKW5_004228"
     CDS             complement(1496..2266)
                     /locus_tag="ACHKW5_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513486.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDVVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDEKLPVVLDAILNRGARFSSMEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRG"
     gene            complement(2311..2829)
                     /locus_tag="ACHKW5_004229"
     CDS             complement(2311..2829)
                     /locus_tag="ACHKW5_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7513487.1"
                     /translation="MAPIACSWRHIPKFWNWMRALKIEKETRMYGTCETLCRELAAQY
                     TENTPLMLVVWSPEEIQALADGMEISLTGHEIRTVLARLEDIPEDQRIESGISSAAVM
                     EIIRNESENRLVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQEVVNQA
                     RTLLKNNTHEND"
     gene            complement(2759..2980)
                     /locus_tag="ACHKW5_004230"
     CDS             complement(2759..2980)
                     /locus_tag="ACHKW5_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513488.1"
                     /translation="MNYAGHEKLRAEVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(2981..3664)
                     /locus_tag="ACHKW5_004231"
     CDS             complement(2981..3664)
                     /locus_tag="ACHKW5_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO7513489.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCIAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(3740..4051)
                     /locus_tag="ACHKW5_004232"
     CDS             complement(3740..4051)
                     /locus_tag="ACHKW5_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513490.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(4048..4950)
                     /locus_tag="ACHKW5_004233"
     CDS             complement(4048..4950)
                     /locus_tag="ACHKW5_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7513491.1"
                     /translation="MYFSGEPAKIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(5060..5350)
                     /locus_tag="ACHKW5_004234"
     CDS             complement(5060..5350)
                     /locus_tag="ACHKW5_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513492.1"
                     /translation="MLRPFFFSDKGCCRAPLFTAPADAARQKRCLWSVSRVRRARDGR
                     FYRSRLASLTVYASQSPFSDERPSSRFRGITLPSKRRRLRYSTVGLTRYRTR"
     gene            complement(5374..5796)
                     /locus_tag="ACHKW5_004235"
     CDS             complement(5374..5796)
                     /locus_tag="ACHKW5_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7513493.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKTILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            complement(5799..6779)
                     /locus_tag="ACHKW5_004236"
     CDS             complement(5799..6779)
                     /locus_tag="ACHKW5_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO7513494.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ATIPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IIVTLPLGEYLDENDQPNLANIERKKNNVRRAVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQSRDDNEFLTRYIPNAEQREQILTVLRDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            6981..7100
                     /locus_tag="ACHKW5_004237"
                     /pseudo
     CDS             6981..7100
                     /locus_tag="ACHKW5_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(7168..7710)
                     /locus_tag="ACHKW5_004238"
     CDS             complement(7168..7710)
                     /locus_tag="ACHKW5_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513495.1"
                     /translation="MTSLKTSIKTITYLSDIGCLEIQGASLASHKTPLSFLKPSDTLY
                     IVAHGNTSVIGSGSATGPTLNPVALASLLIHKRLPKNFIDIRVLSCASGIHSRTPAFA
                     QRLKEIMKVHGYHSLVVTGYLGEVDVSRDWRLKNDDGMEFYTLRKKGIIPVKDVLSES
                     QRALCGSDLKYALSDFKKRF"
     gene            7721..8949
                     /locus_tag="ACHKW5_004239"
     CDS             join(7721..8036,8036..8949)
                     /locus_tag="ACHKW5_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7513496.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQIVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVP
                     PSKRAHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            9718..10275
                     /locus_tag="ACHKW5_004240"
     CDS             9718..10275
                     /locus_tag="ACHKW5_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7513497.1"
                     /translation="MSTHDSLIELTDTLIQQNGYQGFSYADLAKTLGIRKASIHYHFQ
                     TKTDLGIAYCEYKKAGFLALEATLQQLPAGKARLKGYMEAFLACADTGQMCGVYAMMS
                     DCNLFEPPLQEAVNRLAQTDLRILTAVLLSGKESGELFFSAPAEDVAMIVASSIKGAL
                     MLNRIPPHDACIRTMKALEQLLCRV"
     gene            10584..10874
                     /locus_tag="ACHKW5_004241"
     CDS             10584..10874
                     /locus_tag="ACHKW5_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HFO7513498.1"
                     /translation="MKKTTSTALLALTLGSVLAAASAPAMATGDNMEKCYGVSLKGKN
                     DCKAGAGTTCAGTAKMDYQGNAWKLVPAGTCVKMKSPGSPTGHGQLEAFKNA"
     gene            10906..11832
                     /locus_tag="ACHKW5_004242"
     CDS             10906..11832
                     /locus_tag="ACHKW5_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="HFO7513499.1"
                     /translation="MMTSQTHPCDPSASTASGMWNACLPARGGISLKPEHFRQLLADK
                     PDTGFLEIHAENYLCAGGPFHQALTALCECYPLSIHGVGMSIGGEQPLNTAHLQRVAA
                     LVDRYQPHSFSEHLAWSTHDDIFLNDLLPLPYTQATLNRVCEHIDQVQNTLRRQILLE
                     NPATYVEFSTSTFSETDFIREVIHRTGCGLLLDVNNLYISSVNHNRDPYQMLHQLPLD
                     RVREIHLAGYTESQDGVGARLLIDSHDSPVTVPVWQLYESALTGTGAVATLIEWDASI
                     PPLSVLLGEAAMAENRMKEGARHAKQSVPGTA"
     gene            11801..12559
                     /locus_tag="ACHKW5_004243"
     CDS             11801..12559
                     /locus_tag="ACHKW5_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HFO7513500.1"
                     /translation="MQNSLYQALLEPHSPPPVGLTVWNGSDPAVRFAVYRNNIMASLT
                     EALAENCPVLQRQVGDEFFRAMVAEFIRQHPPSSPVLAGYGHALPAWIASFSPLAEWP
                     WLADLTRLELLFIDALHAAETTSLVSEEQLVRLAQTGLVSLTAGLVPSVRLFSSPYAI
                     YSLWAAHQEEEPLFVDPLQPEQVLLFRLGDDVRITLLSPADFSFMVAVHAGNNLAQAT
                     LQAREHDCCFEPGSLLLRLHQNRLLGWLACTEEK"
     gene            12561..13088
                     /locus_tag="ACHKW5_004244"
     CDS             12561..13088
                     /locus_tag="ACHKW5_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7513501.1"
                     /translation="MALMRSFLQRGQALLQDSLIPLLARTGLAATFWLSGQSKVEGLV
                     LDILGGMPPELDVPHITRSAVALFATEYRLPFLAPETAALLAAGAEHIFPVLLVLGLA
                     TRFSALVLAGMTIVIQVFVYPDAWPVHATWLTAQMYLLAYGGGKYSLDNLLKRTYRRR
                     HQNVMQKRNNPMMTL"
BASE COUNT         3246 a         3663 c         3413 g         3049 t
ORIGIN      
        1 atcacttgtt tctgctgccc tgtaagatgt agtgaccgcc gaggaaaacc gcctgctgat
       61 tcaccacaaa atcgcagatt tcatcaacaa tctgcgcggt ggtgagcggt ttctgcccca
      121 tttcacgacg ccgcgcatta atcacggctt tgtaattgcg cagaatttcg cgggtttctg
      181 tgctgatatt catgacatgt gctccccgtc cagcgcctgt aacacaacgt ccattgcttc
      241 gcgttcttcc ctgacgaacg catcaccgtc gcccccgttc tcactcccga cggcatacag
      301 gcgcttcatt tcactgcctg ccagcgccat cactttttcc agcatcaccg ccgggactgt
      361 cacctgacgg ctggcgtgct tttcctccat cagttcacga acaacgtcgt gaacaacgct
      421 gacatccgca ccatgctcaa aggcatcgtc cagccgctgc atgacggttt ccagctcatc
      481 gtctgtcaga ctccagtcct gggctatgtg acgcacatcc ccacgggtca gcactatggc
      541 aaaaacgcat tcactgtccg gcacgtctgt atccagtaca tcgcggataa tttctttaac
      601 agtacagtac atgttctcgt ctcctttaca ggggcggcat tgccgccccg tccgttaatc
      661 agtccagcgc cgcgtaaatc gcgttgcatt cagggtgaaa ttcgatgtgg ctccacaact
      721 gtgcatcgcg catcctgtac aggtgtgtca gccctgcatc cccgctgtcg tggtacagcc
      781 acaactggcg gttaatcaca agggaggtaa ggatgatgcc tgcggcatca gcactcacgg
      841 tacggtcaaa ccagttttca ccgttcacca tgtggaaacg gtcgccatcc ggcatcatgt
      901 aaccgccacc gtccggcagg cggatgaagt gccagaatcc gccttcatat tccggcatca
      961 tttttttcgc cagcgcgcac acagacgctt cgacatacag aaaatcctgg ccaaacaggt
     1021 aaggcagaaa aaacagacgg tcgcactctt caacattcag agtgacaggt ttcgcatact
     1081 gcatgggatt tcctccgttg ttcacatatt ccgcgacacc gggcggtgcc gtgcctccat
     1141 ctttgccggg cctgcagggt tgcaagggcg caagggaagc cgcagcctga cgcacacggc
     1201 tggcgggagt tggagggtga gaccgcgaaa tctgagcggg tgaacgaccg gaaaatcacg
     1261 gcagacggga aggagggacc gggtgaccgc agcggttcac cttaccttgc aacccgcccg
     1321 cagaagcccg gctacgatgg aggcacggca cagcccgcac acgacgctga ggccacataa
     1381 ctgaaaaccc acaaggccat gccccgcatg gccgggtaaa acggagcctg cggcgacatc
     1441 cttttgctct ttccggggac cggcagaaaa aaagcgggga ttttccccgc catcatcagc
     1501 ccctgatttt tgccagtgca tccgccatgc tgcggatttc ggtccgggct tcccggacca
     1561 cctcccgcag aacaccacgg tcaaaccagc ggcggggcgg ttcatccggt atacgcagca
     1621 catcgcaggc cagcccactg gacaggatat gctcaataca atcgctgacc agatacattt
     1681 ccatcgacga aaaacgagcg ccccggttca ggatggcatc gagcaccacg ggcagttttt
     1741 cgtcgtcttc atcgagccag caggggtgat cgctttcctt cacaaccagg cgaaagcggc
     1801 gcgtctggcg ggtcagcaac gtctgaccgg ttgtggtcag cgccgccctg atacgccgcg
     1861 catcttctgc cacccggcaa ctgacactgc gtaccacatc gtgcaccgca tcaagaactg
     1921 ccctgatttc gtcttcggtc agccattctc cggcatacgg gaaagacacc tgcgttccgt
     1981 ctgccggaag taccggcagc gactgccact gactgccgga cagaacaagt tccgccccgg
     2041 ttccggtcag tttctgaatg gcagaggcca ggagcggcgg cagtacgccg ccgtgacggt
     2101 tgataagtaa cgtcgccagc cggggcgcat ccagccgcac gtaaatgctg atccagcccg
     2161 gatacttcgg gttacgctgg tcaacatccg tttcttccgg ccagccctgc gccagcaaca
     2221 ggttgctggc gtggcgataa atcagtgcat tcagcgtttc attcataatt catgaactcc
     2281 ctgtatcgcc gcccggaggc ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag
     2341 cagggtgcgg gcctgattaa ccacctcctg acggcgggtg acgcattccg ggacggcgag
     2401 gccatgatcc cgggcggccc attcacgttt ccacaatgcc tgttcagcgg tctgaatcag
     2461 ggacgccagc agttcagcag ggacggtcac caggcggttt tcgctctcgt tgcggataat
     2521 ctccatcacc gcagcggaag aaataccgga ctcaatccgc tggtcttccg ggatgtcctc
     2581 caggcgtgcc aggacggtcc tgatttcatg accggtcaga gaaatttcca taccgtcagc
     2641 cagtgcctga atctcttccg gggaccagac aaccagcatc agcggcgtgt tctccgtata
     2701 ctgcgctgcc agctcacggc agagcgtttc gcatgttccg tacattctcg tctccttctc
     2761 aatctttaaa gcacgcatcc agttccagaa tttcggtata tgcctccatg aacaggcgat
     2821 tggcgcgata cagcgtctga cgtgtcaggc gttcaacaag cacatcggaa tcaaagcgac
     2881 agcgatgctc catatcgttc agcctggccc gaaggtcaca catggtattg gccacctccg
     2941 ccacttcggc gcgcagtttt tcgtgtccgg catagttcat ttacgcagcc tccggttcaa
     3001 accagttatc attcgcggcc ccctgctgca tggcccgttg cacggcggcc agtcgttgct
     3061 gtccggcacg gtgatactgc tcaagcagct cgataccgat ataccggcgt ccggactgga
     3121 gggcggcgat gcaggttgag ccgctgcctg caaacgggtc cagcacaatc gcgttcgggt
     3181 gtgtgaagct ctcaatgagc ggttgcaggc tggtaacggg cttttccgtc gggtgatgac
     3241 gattgcctga atatttccag cccagcacgt cgggcaatgg cttctgaggc aacgccggac
     3301 ggccttttgc caggatgtag gcgcattcgt ggcgatagcc cacatatgca gccttcgatg
     3361 tgtagttttt ggtgaagacc aggtggccaa ccacgctgaa tcccgcattt ttccaggcag
     3421 acataaagcg atcgacgcga ttccagccat agaagctcac cattaacgcg tcttttttga
     3481 gtacgcggta catctcatta caggccggtt gcagccattc atcggtttta tcgccggcaa
     3541 tggtacgccc ctgacggtca cgaaaaccga cgaggtacgg cgggtcggtg aggatgaaat
     3601 caatggcgtt atcagggata cgggccataa catcgatgca gttaccgagg acaaaacggg
     3661 acatacaaaa ctccttctct tacagagggc accgggaggg cacccctgtg gtgcccgttc
     3721 cccgctgcct gtgggctgtt caccgcgtcc ggtaacgggt caggccgacc gtggaatacc
     3781 gcagccgtcg tctttttgac ggcaaggcta tgccgcgaaa gcggcttgaa gggcgttcat
     3841 cagaaaaggg cgacgggctg gcgtacactg tcaccgacgc cagccgggag cggtaaaagc
     3901 gcccgtcccg tgcccgccgg acgcgggaca ctgaccacag gcagcgttta tgccgcgccg
     3961 cgtcagccgg cgcagtaaaa agaggagccc ggcgacatcc tttaccggaa aagaaacggg
     4021 cggttgcccg cccgtctccg tcatacttca tccgccataa tgcgccacct tgtcgactat
     4081 ccatgcaggt ccggtgactt ccggcagctc atcgtcatct gtcggggaag accattccag
     4141 ttccccccac agcacatcaa caagctctcc gcgctcatac aactgccagc cacagaaatt
     4201 gcagccctgt tcggcgtacc agtgctccag cgtgcagccg aaacggcggc ttaattctgc
     4261 aacaacgtcg ctttccggct gacaccacgg cgtgtcgaaa tctacctgaa tgaagttttc
     4321 cccctgactg ctgatattca ggtcataccc acaaggccac ttcacgccat accgttctgt
     4381 ataccagtga tatgcagaag gaacaccgtt cagaacaccg ccgttaaagc cattcacttc
     4441 aatatccagg cgggtcggca aaaccgtcag caggtcaaac ggcatggttt tttccggcag
     4501 tacgttgtca cacagacggt tccaccacac cgatacatcc tcgttacccc agatgtcaca
     4561 ccagtcacct ctttttgcgg tgaaatgcac agtgatagtt tccctgacat catccggtaa
     4621 ctcttcccag cgacgctgtc cagtaccgct ctgcagccag agttcatgca gcatcaggca
     4681 gttttgctca tccagcagta caccgttctg caggtgtgtc agccagcggg tgaacgcgat
     4741 attttccggt gatacagcac cacgtccggc agccgtcact ccggggcact gctcagagcg
     4801 gatattttca gtgatctgca gaagtccggc acttccggcc agaaacagct gaataccttc
     4861 atttgtggcg cggcgataaa acggtgtgac tgcaccgctg gccagtcgtt taatctcagc
     4921 aatctttgcc ggttcaccag aaaaatacat acgattcgag caccagtttg gcatgggaat
     4981 tttcctctca cttctgcttc agcagagagc accgggaggg cgcccctgta gcgcccgttc
     5041 cccgctgcct gtgggctgtt caccgcgtcc ggtaacgggt caggccgacc gtggaatacc
     5101 gcagccgtcg tctttttgac ggcaaggtta tgccgcgaaa gcggcttgaa gggcgttcat
     5161 cagaaaaggg cgactggctg gcgtacactg tcaacgacgc cagccgggag cggtaaaagc
     5221 gcccgtcccg tgcccgccgg acgcgggaca ctgaccacag gcagcgtttc tgccgcgccg
     5281 cgtcagccgg cgcagtaaaa agaggagccc ggcaacatcc tttgtcggaa aagaaaaaag
     5341 gacgcaacag cgtcctttaa cagagttcag ttcttattca tcatccggaa acaagttctt
     5401 aatgttgttt cttgtttcca ggagattctg atcgactgga gcagatattt cttgcgtagc
     5461 agaatcaacc gcaggcagga tcgtttttgc attattaccg gcagactgca gaaatgcctc
     5521 cagcaaacga acaacttcac cattttcctg ttgtggtagc gtactactga taattcgttg
     5581 aatatcctgc atagacgtgc tgtctgtcag gaattcagct atcaggaacg gcaatcgttc
     5641 atcctgtttc atcaatgcag caccacacaa catcatggcc cgcattgccc gaccacgttc
     5701 tttctgaggc aatttttcaa gaaacctgtc agctacccga tcagctgcat ttcgttccgg
     5761 ataaatataa aaagagcact tacgattatc agccatactc atcccttcat tgccatcatt
     5821 cccaacacca gatcgaactg aggattgtca ctgacaaaga aacggttatc acccactcct
     5881 gtcgcttttt ttactgcccc agccacaagg ctggcaccac cgccaacaac cataacatga
     5941 gtaaaaccag caaagcgtcc tactgaatcc gccacacgat gctgcagttt tttttcccgg
     6001 tcacgtaata ctgtcaggat ctgctcacgc tgttctgcat tcgggatata acgagtcagg
     6061 aattcattgt catcccgact ctggattaga cgatctgcaa aataagaact ggttcgtgta
     6121 ctcacattca catccatcgc atgcttaact gcttcagtaa tgatggaaac gcctgtcttg
     6181 gggtcacaat aagtttttgt gaaaccggac attttgctgc ggacatgtgc aatatcaagg
     6241 gtcgttccac cgatatcaac gatcaacagc gactccagat cattcagatc tttcagcacg
     6301 tcaaaaccgg caggcacgga ctcaggcaag acactgactt tacgaatggt aaagttttca
     6361 cgcccctgaa ctgttacagc tcgtctgaca ttatttttct tacgttcgat attagccaga
     6421 tttggctgat cgttctcatc cagatactca cctaaaggta aagtaacaat aatatcaaca
     6481 ggttgcggag caatacctga ctgcatcaac gcatgatgaa tagcaaccac attaacgtcg
     6541 ctgtactgat aacgcgtttc tgtggtgcgc actgcatccg gactcaattg atcaaagctg
     6601 tatttctctc catcaatctc atagtttgcg gaagcgggta tagtggcatc agtcagtccc
     6661 tgcagcgtca tagaccattc cggtttaaaa ctattcggac taattaacgt tttcactgca
     6721 ccgtcttcaa cccaggccag tttgatgttg gtagaaccgt catctacaaa gatacgcatc
     6781 cagaacctca cagaataaaa tggatttttt tagaaaaata tggtgccgaa aaagattatc
     6841 acctaaaaac acgcatcagt aaagacctga tatcgcccac aaacagaaaa ataaagaaaa
     6901 ataaagataa aaatcgacta aaacagcacg caggaaacaa acaagaacaa taaaggacaa
     6961 atacagttaa aattcagaaa aaactgaaca atgtggggcc agaaaaatgg ctacgttatg
     7021 tcattgagca tatccaggac tggccggcaa accgggtacg cgatctgttg ccctggaaag
     7081 ttgatctgag ctctcagtaa atatcaatac ggttctggcg agccgcttac ttgcataacg
     7141 gctctgacgt cagtgcacta ctcctattca aaatcgtttc ttgaaatcag aaagggcgta
     7201 tttcagatca gaaccacaaa gcgctctctg ggattcactt aatacatcct taacaggaat
     7261 aatccctttt tttcttaagg tataaaactc cataccatca tcatttttta atctccagtc
     7321 tctcgataca tcaacctcac caaggtatcc ggtaacgacc agactatgat acccatgtac
     7381 tttcataatt tctttcagcc gttgtgcgaa ggctggggtt ctcgaatgta tccccgaggc
     7441 gcaggacaac actctgatat ctatgaagtt ctttggtaaa cgtttgtgta ttaatagaga
     7501 ggctaatgct acgggattca acgttggccc agtagctgaa ccagatccaa taactgatgt
     7561 gttgccatga gcaacgatgt ataacgtatc gctcggttta agaaaggata aaggtgtttt
     7621 gtgtgaagct agactggccc cctgaatctc cagacaacca atatcactta aataagtgat
     7681 agtcttaata ctagttttta gactagtcat tggagagcag atgattgatg tcttaggacc
     7741 ggagaaacgc agacggcgta ctacacagga aaagatcgct attgttcagc agagttttga
     7801 accgggaatg acggtctccc ttgttgcccg gcaacacggt gtggcagcca gccagttatt
     7861 tctctggcgc aagcaatacc aggagggaag tcttactgct gtggccgcag gagagcagat
     7921 cgttcctgcc tctgaacttg ctgctgccat gaagcagatt aaagaactcc agcgcctgct
     7981 cggcaaaaaa acgatggaaa atgaactcct taaagaagcc gttgaatacg ggcgtgcaaa
     8041 aaagtggata gcgcacgcgc ccttattgcc cggggatggg gagtaagctt agtcagccgt
     8101 tgtctccggg tgtcgcgtgc gcagttgcac gtaattctca gacgaaccga tgactggaaa
     8161 gatggtcgcc gcagccgtca ctcagatgat acggatgtgc ttctccgtat acaccatgtt
     8221 atcggagagc tgcccacgta tggttatcgt cgggtatggg cgctgcttcg cagacaggcc
     8281 gaacttgatg gtatgcctgc gatcaatgcc aaacgtgttt accggatcat gcgccagaat
     8341 gcgctgttgc ttgagcgaaa aaccgctgta ccgccatcga aacgggcaca tactggcaaa
     8401 gtggccgtga aagaaagcaa tcaacgatgg tgctctgacg ggttcgagtt ccgctgtgat
     8461 aacggagaaa aactgcgagt cacgttcgcg ctggactgct gtgaccgtga ggcactgcac
     8521 tgggcagtca ctacgggcgg cttcgacagt gaaacagtac aggacgtaat gctgggagcg
     8581 gtggaacgcc gcttcggcaa cgagcttccg gcgtctccag tagagtggct gacggataat
     8641 ggttcatgct accgggctaa tgaaacacgg cagtttgccc ggatgttggg gcttgaaccg
     8701 aagaacacgg cggtgcggag tccggagagt aacggcatag cagagagctt cgtgaaaacg
     8761 ataaagcgtg actacatcag tatcatgcct aaaccagacg ggttaacggc agcaaagaac
     8821 cttgcagagg cgttcgagca ttataacgaa tggcatccgc atagtgcact gggttatcgc
     8881 tcgccacggg aatatctgcg gcagcaggcc agtaatgggt taagtgataa caggtgtctg
     8941 gaaatatagg ggcaaatcca gaagctttca caccataaca ttctttattc ttcattttct
     9001 gatacttaac ttcggcatca agaaaagcgt cattttcttc catttgaaat gggaaaaagt
     9061 aaataatcat attacctctc gacatcagaa aaaattaaaa tactccacat gcggaaagct
     9121 ctgagcagga aacatcacca aggacgaaac ctcctctctc tgcaaaggat agttatccat
     9181 ccggggcaac ctataccttt accataactt ccggcatagc caccttgcta caatcaaggg
     9241 gtgttgaaga aacatccgtt tcgtggtgct tttttagtct tttggggatt taaattccta
     9301 tcgatgaacg gggaggagat ctggccctgg atgttaccaa ccgatccccg cagacatttg
     9361 ctttcagaac agtctgaaat atcagtaata tctgcaacag gatgccattc cgggctcagt
     9421 aaggcacttc ttcccctgac ttacgtgtct tcagcaattt gagcacccat gtatactgct
     9481 gcaaaatggc atctgaatcg atcacggttt ggccaatccg tgatcgaata gagcggaata
     9541 cgcaccggtg cggacatcgt gtaatccggg catgtgacgg aagtgctgaa acagacccag
     9601 gagcagcagt catcttcgct ttgcagtaaa ataccagtac agatttcctg cctggcggcg
     9661 tttgtcttcg ggcctgttgt tattcagaat tactatagcc ccccccggga gaaacatatg
     9721 tccacccatg acagtctgat tgaactgact gacacactta tccagcagaa cggttaccag
     9781 gggttcagct atgctgacct ggcgaagacg ctgggtatcc gtaaagccag cattcactac
     9841 catttccaga caaaaacaga cctgggaata gcctactgtg aatataaaaa agccggtttt
     9901 ctggcgctgg aagccaccct gcagcagtta cctgccggga aagcccgcct gaagggctat
     9961 atggaggcct ttctggcctg cgcggacacc ggccagatgt gcggggtgta cgcaatgatg
    10021 tcggactgta acctgtttga gcctcccctg caggaggccg ttaaccggtt ggcacagacg
    10081 gacctgcgca ttctgacggc ggtcctgctg tccggaaagg aaagcggaga actgtttttt
    10141 tctgccccgg cagaagatgt tgctatgatt gtggccagtt cgataaaagg cgccctgatg
    10201 ctgaaccgca taccgcctca cgacgcctgc atccgcacga tgaaagcgct ggagcagttg
    10261 ctctgccggg tctgactcat ccctgcggac gtgtcccggt gatgccgggg cgaccccctg
    10321 tcattttgag cggtccgact ggacgttacc ggagtgatcc ggaacctctc aaggacaggc
    10381 aactcagcat cagccatggt ctgccaggca taactgcctg tatttatttt ccagaccggg
    10441 tgcaacctgc ctcccgtatt gtgaagaaaa tctcgttttc cggacataaa gaaaaaaaac
    10501 acattgagaa cttatctatt agtagatagt tttaaaggca tcagcaatgt gctgataaat
    10561 acaacgactc agaggatatg aatatgaaaa aaacaacctc caccgcatta ctggcactga
    10621 ccctgggcag cgtactggca gcggccagtg ccccggcaat ggccactggc gataatatgg
    10681 aaaaatgtta cggcgtttcg ctgaaaggca aaaacgactg taaagccggc gccggcacaa
    10741 cctgtgcagg caccgcgaaa atggattatc agggaaatgc ctggaaactg gtaccggcgg
    10801 gcacctgcgt gaagatgaaa tccccgggtt cccctaccgg ccatggccag cttgaggcct
    10861 ttaaaaacgc ataatatcca cgacagtcca caaccaggag ccgatatgat gaccagccag
    10921 actcacccct gtgatccctc tgccagcact gcttctggta tgtggaatgc gtgcctcccc
    10981 gcccggggag gcatcagcct gaaaccggag catttccggc aacttctggc agataaaccc
    11041 gatacgggct ttctggaaat tcatgcagag aattatctct gtgccggggg gcctttccac
    11101 caggcgctga cggccctgtg tgaatgctat cccctgtcca ttcacggggt ggggatgtcc
    11161 atcggtggag aacaaccgct gaataccgca catctgcagc gggtggcggc cctggttgac
    11221 cgctatcagc cgcattcatt ttcagagcat ctggcctggt ccacgcatga tgatattttt
    11281 ctgaatgatc tcctgccgtt accgtacaca caggccacgc tgaaccgggt atgtgaacat
    11341 atcgatcagg tccagaatac gctgcgtcgc cagatattgc tcgagaatcc ggccacctat
    11401 gttgaattct ctacctccac cttcagtgag accgatttta tccgtgaggt gatccaccgt
    11461 accggctgcg gactgttgct cgatgtgaat aacctctata tcagcagcgt gaatcacaat
    11521 cgcgatccgt accagatgtt gcatcaactg cccctggacc gggtgaggga aatccatctt
    11581 gcaggctata ccgaaagcca ggacggcgtc ggtgcccgcc tgcttattga cagtcatgat
    11641 agcccggtga cggtgccagt ctggcagctt tatgagtccg ccctcaccgg cacaggtgcg
    11701 gtcgccaccc tgatcgaatg ggatgccagt atccccccgc tcagcgtcct gctcggtgag
    11761 gcagcaatgg cagaaaacag aatgaaagaa ggagccaggc atgcaaaaca gtctgtacca
    11821 ggcactgctt gagccacact cccccccccc ggtggggctt accgtctgga atggcagcga
    11881 tccggccgtc cggtttgcgg tctaccgtaa taatatcatg gcctccttaa cggaagcgct
    11941 ggcagaaaac tgcccggtcc tgcaacgaca ggttggtgac gaattttttc gggccatggt
    12001 tgctgaattt atccgtcagc atccaccctc gtcgccggta ttggcggggt atggacatgc
    12061 tctgcctgcc tggatagcgt ccttttcccc actggctgaa tggccgtggc tggccgatct
    12121 gacccgactg gaactattgt ttatcgatgc gttgcatgcg gcggagacga ccagcctcgt
    12181 atccgaagaa cagctggtgc gactggcgca gaccggactg gtcagtctga ctgcgggcct
    12241 ggttccttct gtccgacttt tcagctcgcc ttatgcgata tacagcctgt gggcagctca
    12301 tcaggaggag gagccattgt ttgttgaccc tctgcagcca gagcaggtgc tgttgttccg
    12361 tttgggggat gacgtgcgga ttacgcttct cagccctgcc gacttttcgt ttatggtggc
    12421 ggtacatgcc ggcaataacc tggcgcaggc aacgttgcag gccagagagc atgactgctg
    12481 ttttgaacct ggctctctgt tgctgcgcct gcaccagaac aggctactcg ggtggctggc
    12541 ctgcacagag gagaaatgat atggcattga tgagaagttt tctgcagcgg ggccaggcat
    12601 tactgcagga cagtctgatc ccccttctgg ctcgtacagg cctggccgcc acattctggc
    12661 tgtcagggca aagtaaggta gaggggttgg ttctggatat actgggaggg atgccaccgg
    12721 aactggatgt tccgcatatt acccgttcgg cggttgcgct gtttgccaca gagtaccgac
    12781 tgccgtttct ggcaccggag actgctgcac tgcttgcagc aggagccgaa catatttttc
    12841 cggttctgct tgtcctggga ctggcaacgc gtttttccgc actagtgctg gcaggaatga
    12901 cgatagtgat ccaggtgttt gtctatcccg atgcctggcc ggttcatgcc acctggctta
    12961 ccgcccagat gtatttactg gcgtatggcg gcggaaaata ttccctggat aacctgctga
    13021 aacgtacgta ccggcgtagg caccagaatg tgatgcagaa aaggaataac cctatgatga
    13081 cgctataatc ttaaataatg tccgggatag catataagct acttcttcag gaaaaggact
    13141 caattgggag ggcatgaaaa accctggggc aatagctatg ccgccatgaa ctatttaaaa
    13201 catcatttga tgaattaaaa aacaatccat atatgcggga acctatagag catggtgatt
    13261 tattatctga taaagagaaa ataaaaacta taataactaa tactataaat agttttgata
    13321 ttcagtttgc agaaaacatg cttaaagctc aaagcagaag ttatcgctat a
//
LOCUS       DAZFYC010000132        13366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFYC010000132 DAZFYC010000000
VERSION     DAZFYC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.132"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..1113
                     /gene="yfdF"
                     /locus_tag="ACHKW5_004245"
     CDS             55..1113
                     /gene="yfdF"
                     /locus_tag="ACHKW5_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO7513502.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIFSTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTE
                     YQNEINQLSAPGVNFDDI"
     gene            complement(1295..2050)
                     /gene="mlaA"
                     /locus_tag="ACHKW5_004246"
     CDS             complement(1295..2050)
                     /gene="mlaA"
                     /locus_tag="ACHKW5_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO7513503.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            2344..3276
                     /locus_tag="ACHKW5_004247"
     CDS             2344..3276
                     /locus_tag="ACHKW5_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO7513504.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAEGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3352..3426
                     /locus_tag="ACHKW5_004248"
     tRNA            3352..3426
                     /locus_tag="ACHKW5_004248"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3385..3387,aa:Arg,seq:cct)
     gene            complement(3868..5115)
                     /locus_tag="ACHKW5_004249"
     CDS             complement(3868..5115)
                     /locus_tag="ACHKW5_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO7513505.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(5187..6101)
                     /locus_tag="ACHKW5_004250"
     CDS             complement(5187..6101)
                     /locus_tag="ACHKW5_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO7513506.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            6317..7750
                     /gene="cscA"
                     /locus_tag="ACHKW5_004251"
     CDS             6317..7750
                     /gene="cscA"
                     /locus_tag="ACHKW5_004251"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO7513507.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(7758..8753)
                     /locus_tag="ACHKW5_004252"
     CDS             complement(7758..8753)
                     /locus_tag="ACHKW5_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7513508.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            8996..9214
                     /locus_tag="ACHKW5_004253"
                     /pseudo
     CDS             8996..9214
                     /locus_tag="ACHKW5_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            9232..10560
                     /gene="dsdA"
                     /locus_tag="ACHKW5_004254"
     CDS             9232..10560
                     /gene="dsdA"
                     /locus_tag="ACHKW5_004254"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO7513509.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(10668..12206)
                     /gene="emrY"
                     /locus_tag="ACHKW5_004255"
     CDS             complement(10668..12206)
                     /gene="emrY"
                     /locus_tag="ACHKW5_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO7513510.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(12206..>13366)
                     /gene="emrK"
                     /locus_tag="ACHKW5_004256"
     CDS             complement(12206..>13366)
                     /gene="emrK"
                     /locus_tag="ACHKW5_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO7513511.1"
                     /translation="EQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIST
                     DDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLAN
                     IVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLI
                     SSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKETWLALKRTDIRSPVTGYI
                     AQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVV
                     FHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMT
                     ATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3856 a         3163 c         2988 g         3359 t
ORIGIN      
        1 tatttcatta ggtaatatat atttgtaaca aatcaatcaa aatggaataa aatcatgcta
       61 ccatctattt caatcaacaa taccagcgca acttacccag aatccattaa tgaaaataac
      121 aatgatgaag ttaatggatt agtacaagag ctcaaaaacc tttttaatgg taaggaagga
      181 ataagcacct gtattaaaca tctacttgag cttataaaaa acgccatacg agtaaacgac
      241 gatccttata gatttaatat taataattcc tcagttactt atattgatat tggctccaat
      301 gatacagacc atattactat tggtatcgac aaccaagaac caatagaatt acctgcgaac
      361 tataaagaca aagaactcgt ccgtactatc attaatgaca acatagttga gaagactcat
      421 gatatcaata acaaggaaat gatcttcagc gcattaaaag aaatatatga tggagatcct
      481 ggttttatct tcgataagat atcacacaaa ctcagacata cggtaacgga atttgatgag
      541 agcgggaata gcgaaccaac ggacttattt acctggtacg gtaaagataa aaaaggcgac
      601 tctctcgcta ttgtaattaa aaataaaaac ggaaatgatt acttatctct cggttactac
      661 gatcaggacg actaccacat tcaaagagga attcgtatta atggtgatag tctcacccaa
      721 tattgtagtg aaaaagccag gaatgcttca gcgtggtttg aaagcagtaa agctatcatg
      781 gcagaatcat ttgcaacagg ttccgatcat caggttgtaa acgagctcaa cggggaaaga
      841 ctgagagaac ctaacgaagt ttttaaacgt ttaggtcgag caataagata taattttcaa
      901 gtggacgatg caaaatttag acgcgataat gtaaaagaaa tattttctac tttattcgct
      961 aacaaagttg acgttgacca tcctgaaaat aaatacaaag actttaagga tctcgaagat
     1021 aaagttgaaa aaaggcttca aaatcgccag acggaatatc aaaatgaaat taatcaacta
     1081 tctgcgccag gtgttaattt tgatgatatt taaaattatt tttccagttt tttgattttt
     1141 caactcctga atatcggaaa tagtcagtga cgaacatcag tatttttaca tcattacgtc
     1201 cgggtcattg tcggtttatc tccttttact tgtggtaatt gagggataac aacaaaaaag
     1261 gtgagtattg cgactcacct tttttatttg tttcttattc agaatcgata tcttttaaat
     1321 catcctgaat cgcttgcgcg ttcggatttt cctgcggttt gagttcgccg ccattagcga
     1381 tgaaatcatg acgctggaag tacgcttcgc gcaccataat ataaggatcg gacgactgac
     1441 gcagcagacc atcggaatcc aacaactgcg cacgagtttc tatcccttca agcgtccatt
     1501 tacccacaga catcggccag gtcagccagg aaagaaccgg gtaaagacca tccgccatat
     1561 caccaccgtc atcacgcagc gtgaagctac cgtagaacgg taactggacg taaggcccat
     1621 aacccacgcc ataatgacca agcgtactac cgaagcggtg aggttcagtc cgttgcagtt
     1681 tcgggttcgc catccctgca acatcaataa aaccgcccat ccccaaaatg gtgttcagga
     1741 aaaagcgggt aaagtggacc atcccctgat aagggtcgcc ctgcaagaag tagttaacca
     1801 tcaccgcagg ttcttcaagg ttgccagtaa agttgctcaa accgttacgc gccggttgcg
     1861 gaacataatc acgccaggcg acagcgaccg gtcgaacaat atacgggtct aatacattga
     1921 agttgaagtt gtacatggtg cggttgaacc cttctaacgg gtcagaacgc ccttgctgat
     1981 ctgtaccgga actcgcacac cccaccagaa gcgtagttcc cagagcaagc gccgacaggc
     2041 gaagcttcat aaatgtctcc ctgttttttt atggcttatg cagtttgcca tccatgacgg
     2101 aacgataccg tatccgcctg tttaggtgtg ggcgattgta acagcacgtc aactgatgtc
     2161 cagacgccct gatttgctga tttgatcata gcctggtaat cgccgccctg taggctactt
     2221 gattctatag aaacagaaaa aggcaaacgc agccttttct acattttcag agtaactccc
     2281 gccagttgcg agcaaaaaag ccgctacgct ttagctatac gtgctaattc aagagaagag
     2341 accatggaca acgacaaaat tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa
     2401 aaagagcgcg gcaaaaaaat agaaatagat gaagaccgac tcccctcccg ggcgatggca
     2461 attcatgagc atatccgcca ggatggtgaa aaagagctgg aacgcgacgc aatggcgcta
     2521 ctgtggtcag ccattgcggc gggtctgtcg atgggcgctt cgctactggc agaagggata
     2581 tttcatgtcg aactggaagg agtgccaggc agcttcttac tggagaatct cggttatacc
     2641 tttggtttta ttatcgtcat tatggcccgc cagcaattat ttaccgaaaa caccgtgact
     2701 gcggtactac ccgtcatgca aaaaccgaca atgagcaacg tcggcttact tatgcgatta
     2761 tggggcgtcg tgctgctggg taatattctc gggacaggta ttgctgcatg ggcatttgaa
     2821 tatatgccta tcttcaatga agaaactcgc gatgcatttg tcaaaatcgg catggatgtg
     2881 atgaagaaca cccccagcga gatgtttgcc aacgcgatca tttccggctg gctgatcgcc
     2941 actatggttt ggatgtttcc tgctgcgggt gcggcaaaga ttgtggtgat tatattgatg
     3001 acctggctta ttgcactggg tgacaccacc cacatcgttg tcggttctgt tgaaatcctc
     3061 tatctggtgt ttaacggcac gctgcactgg agcgatttca tctggccctt cgcactacct
     3121 actttagcgg ggaacatctg cggcggcacc tttatcttcg cgttaatgag tcatgcacag
     3181 attcgtaacg acatgagcaa caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg
     3241 gaaaacatta agaaaaatga taaaaaccca gcataaatgg cgagggttta agcaatcgag
     3301 cggcagcgta cttaccccgc agtccattag cgggtatact catgccgcat tgtcctctta
     3361 gttaaatgga tataacgagc ccctcctaag ggctaattgc aggttcgatt cctgcagggg
     3421 acaccagata cccttcaaac gaaatctacc ttcaccccgt aaaagatggg tttggcagca
     3481 cacttgcctt atatctactc atttttactg caacaggttg aaatctcagc actgtcagaa
     3541 agcgctgatg actaaacagc cctgggccgg gcgatgtaac catcacacag aatcctgata
     3601 gcgaaatatg gcgtgactcg atacttcact ccgcaatgca ttccttgatg aattcgcagg
     3661 accgtgatac acgggacagg tcactgaatg acgacaatgt cctggaaatc agcgaaccgc
     3721 gcatctgaag tacatttgag cgactgtacc agaacatgaa tgaggcgttt ggattaggcg
     3781 attattagca gggctaagca ttttactatt attattttcc ggttgaggga tatagagcta
     3841 tcgacaacaa ccggaaaaag tttacgtcta tattgctgaa ggtacaggcg tttccataac
     3901 tatttgctcg cgttttttac tcaagaagaa aatgccaaat agcaacatca ggcagacaat
     3961 acccgaaatt gcgaagaaaa ctgtctggta gcctgcgtgg tcaaagagta tcccagtcgg
     4021 cgttgaaagc agcacaatcc caagcgaact ggcaatttga aaaccaatca gaaagatcgt
     4081 cgacgacagg cgcttatcaa agtttgccac gctgtatttg aagacggata tgacacaaag
     4141 tggaacctca atggcatgta acaacttcac taatgaaata atccaggggt taacgaacag
     4201 cgcgcaggaa aggatacgca acgccataat cacaactccg ataagtaatg cattttttgg
     4261 ccctacccga ttcacaaaga aaggaataat cgccatgcac agcgcttcga gtaccacctg
     4321 gaatgagttg agataaccat acaggcgcgt tcctacatcg tgtgattcga ataaacctgc
     4381 ataaaagaca ggaaaaagtt gttgatcaaa aatgttatag aaagaccacg tccccacaat
     4441 aaatatgacg aaaacccaga agtttcgatc cttgaaaact gcgataaaat cctctttttt
     4501 tacccctccc gcatctgccg ctacgcactg gtgatcctta tctttaaaac gcatgttgat
     4561 catcataaat acagcgccaa atagcgagac caaccagaag ttgatatggg gactgatact
     4621 aaaaaatatg ccggcaaaga acgcgccaat agcatagcca aaagatcccc aggcgcgcgc
     4681 tgttccatat tcgaaatgaa aatttcgcgc cattttttcg gtgaagctat caagcaaacc
     4741 gcatcccgcc agatacccca agccaaaaaa tagcgccccc agaattagac ctacagaaaa
     4801 attgctttgc agtaacggtt cataaacgta aatcataaac ggtccggtca agaccaggat
     4861 gaaactcata caccagatga gcggtttctt cagaccgagt ttatcctgaa cgatgccgta
     4921 gaacatcata aatagaatgc tggtaaactg gttgaccgaa taaagtgtac ctaattccgt
     4981 ccctgtcaac cctagatgtc ctttcagcca aatagcgtat aacgaccacc acagcgacca
     5041 ggaaataaaa aagagaaatg agtaactgga tgcaaaacga tagtacgcat ttctgaatgg
     5101 aatattcagt gccataatta cctgcctgtc gttaaaaaat tcacgtccta tttagagata
     5161 agagcgactt cgccgtttac ttctcactat tccagttctt gtcgacatgg cagcgctgtc
     5221 attgcccctt tcgccgttac tgcaagcgct ccgcaacgtt gagcgagatc gataattcgt
     5281 cgcatttctc tctcatctgt agataatccc gtagaggaca gacctgtgag taacccggca
     5341 acgaacgcat ctcccgcccc cgtgctatcg acacaattca cagacattcc agcaaaatgg
     5401 tgaacttgtc ctcgataaca gaccaccaca ccttctgcac ctttagtcac caacagcatg
     5461 gcgatctcat actcttttgc cagggcgcat atatcccgat cgttctgtgt ttttccactg
     5521 ataagtcgcc attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc
     5581 aaacacaagc ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag
     5641 ctgacaaaac ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa
     5701 ggctcggcag acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc
     5761 aagtctgtcg tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt
     5821 tccccttgat cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc
     5881 agatacgtga tatcgactcc ctcagttagc agcgttcttt gcattaacgc accaaaagga
     5941 tcatccccca cccgacctat aaacccactt gttccgccta atctggcgat tcccaccgca
     6001 acgttagctg gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga
     6061 tctacgaccg catcccctaa aacccatact ttggctgaca tttttttccc ttaaattcat
     6121 ctgagttacg catagtgata aacctctttt tcgcaaaatc gtcatggatt tactaaaaca
     6181 tgcatattcg atcacaaaac gtcatagtta acgttaacat ttgtgatatt catcgcattt
     6241 atgaaagtaa gggactttat ttttataaaa gttaacgtta acaattcacc aaatttgctt
     6301 aaccaggatg attaaaatga cgcaatctcg attgcatgcg gcgcaaaacg ccctagcaaa
     6361 acttcatgag caccggggta acactttcta tccccatttt cacctcgcgc ctcctgccgg
     6421 gtggatgaac gatccaaacg gcctgatctg gtttaacgat cgttatcacg cgttttatca
     6481 acatcatccg atgagcgaac actgggggcc aatgcactgg ggacatgcca ccagcgacga
     6541 tatgatccac tggcagcatg agcctattgc gctagcgcca ggagacgata atgacaaaga
     6601 cgggtgtttt tcaggtagtg ctgtcgatga caatggtgtc ctctcactta tctacaccgg
     6661 acacgtctgg ctcgatggtg caggtaatga cgatgcaatt cgcgaagtac aatgtctggc
     6721 taccagtcgg gatggtattc atttcgagaa acagggtgtg atcctcactc caccagaagg
     6781 aatcatgcac ttccgcgatc ctaaagtgtg gcgtgaagcc gacacatggt ggatggtagt
     6841 cggggcgaaa gatccaggca acacggggca gatcctgctt tatcgcggca gttcattgcg
     6901 tgaatggacc ttcgatcgcg tactggccca cgctgatgcg ggtgaaagct atatgtggga
     6961 atgtccggac tttttcagcc ttggcgatca gcattatctg atgttttccc cgcagggaat
     7021 gaatgccgag ggatacagtt accgaaatcg ctttcaaagt ggcgtaatac ccggaatgtg
     7081 gtcgccagga cgactttttg cacaatccgg gcattttact gaacttgata acgggcatga
     7141 cttttatgca ccacaaagct ttttagcgaa ggatggtcgg cgtattgtta tcggctggat
     7201 ggatatgtgg gaatcgccaa tgccctcaaa acgtgaagga tgggcaggct gcatgacgct
     7261 ggcgcgcgag ctatcagaga gcaatggcaa acttctacaa cgcccggttc acgaagctga
     7321 gtcgttacgc cagcagcatc aatctgtctc tccccgcaca atcagcaata aatatgtttt
     7381 gcaggaaaac gcgcaagcag ttgagattca gttgcagtgg gcgctgaaga acagtgatgc
     7441 cgaacattac ggattacagc tcggcactgg aatgcggctg tatattgata accaatctga
     7501 gcgacttgtt ttgtggcggt attacccaca cgagaattta gacggctacc gtagtattcc
     7561 cctcccgcag cgtgacacgc tcgccctaag gatatttatc gatacatcat ccgtggaagt
     7621 atttattaac gacggggaag cggtgatgag tagtcgaatc tatccgcagc cagaagaacg
     7681 ggaactgtcg ctttatgcct cccacggagt ggctgtgctg caacatggag cactctggct
     7741 actgggttaa cataatatca ggtggaacaa cggatcaaca gcgggcaagg gatccgcgtc
     7801 actcttcccc cttcacgacc ttcaataata tgcaatgcag cttcccgccc gataatgtca
     7861 tgtggaagct gaattgtggt cagcggcggt aaaaacagat gcccgacgcc aaccagatta
     7921 tcaaagccca ttacggcgac atcctgcggg atacgtaccc ccttcgccag gagaacctga
     7981 taagcgacaa aggctgcgcg atcgttacca catatcagaa catcaaaatc tggtttgccc
     8041 ggtttgaagt gggcattgag taaacttgcg agatcggtgt agtgatcatc acctgttgcc
     8101 atgtgaaatt gtttcacctc agccagatct cgtccagcat cacgccaggc ctgctcaaat
     8161 ccctgccgac gataccctgt tgccaacgca ctttccggta gccagaagca taacggttga
     8221 cgatagcccg ccgcgagcaa atgctgtgtt gattcatatt gtgcagtgta atcatcaggg
     8281 atataactgg gtaacgctgg gtcatccgcc acacagttcg ccaatacaat attttcacca
     8341 tacagagact caggcagcgt gatatgtcgc agccccattg tagtatagat aatgccatcc
     8401 ggacggtggg caagcagctg acgtgccgcg cgggcagcgt catcttcaga aaaaatattg
     8461 attaaaaaac tattccagcc gaactcgctg gcggtttgct caatggcaag cagaatatca
     8521 acagagaaag gagtggtagc cgtgtcctgc gccagcacgg cgagagtcga cggcttacgt
     8581 ccttgagcgc gcatcttacg ggcggaaaga tcaggaacat aattcagggt ctggattgcc
     8641 tgcaatacgc ggtcacgcgt tgcaggacgc acagattctg cattatgcat cacccgggag
     8701 actgtcatca tcgacactcc cgccaggcgt gcgacatcct ttaatgaagc catacccaag
     8761 ccgtttgccg taaaacgggc actgtagcag aaacagacgt cactggcgag atccaacgat
     8821 attctcaacg ccctatcacc caacacggca atacaataaa aaataacaat aattgccgga
     8881 taattgtact caattccgcc tctgttctcg ccaacgagta accatatagg caagtaagta
     8941 aaacatgcca actggcatcg ccaacctgga gaagaaaata taaatatgga tcattgtgct
     9001 gccgctgtat cccgacatca gcccggaaat tattgcgatt gctatcggtt caggtgcaat
     9061 tggctgcacg atcgttacgg actcgctttt ctggctagtg aagcaatatt gcggcgctac
     9121 gctcaatgaa acatttaaat actatacgac agcgacattt atcgcttcag tcgtcgctct
     9181 ggcgggcaca ttcctgctgt catttatcat ctaagcgcaa agagacgtac tatggaaaac
     9241 gctaaaatga actcgctcat cgcccagtat ccgttggtaa aggatctggt tgctcttaaa
     9301 gaaaccacct ggtttaatcc tggcacgacc tcattggctg aaggtttacc ttatgttggc
     9361 ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc accctatctg
     9421 gcaaaagcat ttcctgaaac tgctgccact ggggggatta ttgaatcaga actggttgcc
     9481 attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag cgggcaactg
     9541 ttactgaaaa aagatagcca tttgcccatt tccggctcca taaaagcacg cggcgggatt
     9601 tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct gacgcttgat
     9661 gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca atacagcatt
     9721 gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc ccgcattggc
     9781 tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc gaaactgcgc
     9841 agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt cgaggaagga
     9901 cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa ttcccgcacg
     9961 ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc ccagcaaggc
    10021 cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt tggcggtggt
    10081 cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca ctgctttttt
    10141 gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt acacgatcag
    10201 atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct tgcagttggt
    10261 cgcgcatcag gctttgtcgg gcgggcaatg gagcgtctgc tggatggctt ctataccctt
    10321 agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg tattcgtctt
    10381 gaaccttcag cactggcggg tatggccgga cctcagcgcg tgtgtgcatc agtaagttac
    10441 caacagatgc acggtttcag cgcagaacaa ttgcgtaatg ccactcatct ggtgtgggcg
    10501 acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa aggccgttaa
    10561 taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc cgatggcgca
    10621 gacttaagat ccccggtctt acccgctata accccctcct tttatcatca cccaacgcct
    10681 ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat aaaagcaata
    10741 gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt tatttcatta
    10801 ttaatttcat taagaactcc tgaaagcgaa ccatagtatt tatccataat ttgtgacgat
    10861 gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg gtgtaacgat
    10921 tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga caaggtacga
    10981 aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc tgaaaacgaa
    11041 atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa ctgcggcaaa
    11101 atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta atagcaaacc
    11161 gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt gccataacgt
    11221 cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc aagtccggcc
    11281 catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac gatcgctcca
    11341 gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt acgggactta
    11401 aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac taaagagatc
    11461 agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa ccaatccaga
    11521 tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa cagggtcagt
    11581 cctggtagat tcattttgac cggtgaagtt tcagtttctc ttcctttaag taaggttaag
    11641 cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca accccagcta
    11701 aagttatcgc aaatataacc gcccaatatc ggcccacata tcggagcgat aatcacggtc
    11761 attgaccata atgccagagc aaatgttctt ttttctggtg gataattcct taataataaa
    11821 ctctgtgaca gtggaattaa cggccccgcc attaatccct gaacgactct aaaaaatatc
    11881 agcacatcaa gattggtcga taaactacac attaaggaag acagcgaaaa aaaagtgact
    11941 gaaagtaaaa ataaccttaa ttcgcctatt ctttgtgcca gcctgccggt aacagggatc
    12001 gcaatggcat ttgctacgcc aaacgaggtg ataacccagg tgccttcgtc tgttgatgct
    12061 cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc caacatttgc
    12121 ataaacgtcg ctaatgacaa tgcaatagta acgcaccata acgtcccacc ggttaatggt
    12181 gccggagtcg atttagtgat tgccattaaa gttgtccatt atgcgaaata atgttgctaa
    12241 tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa gccggcatgg
    12301 acgtcacggt tgaagccaga tcaggcatct cagcaatgtc ttcgttcttc gtatcaatag
    12361 ttgcagtcat cgataaacca atgcgtaagg ggtgttccat gagttctttt ggatcaagag
    12421 aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca ttttgtgcag
    12481 gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga ccatgaaaca
    12541 caacattttc accataaaga tcgctgataa tattgactga ttgaccaatc cgtacatccg
    12601 tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt acgacagcca
    12661 ttaacgattg tccgggactc actgtttcgc cgacctgaac actccgctgg gcaatatagc
    12721 cagtaaccgg actccttata tccgtacgtt taagcgccaa ccaggtttct ttagttgcat
    12781 ccgccgcttc cacgacttgt ggctgacggt ttaatggtgt gttcattact aaagctttat
    12841 tcgctttata agcctggata gcggcattca gtgccgcttt gctacttatt aacgtatctt
    12901 tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggt actcgacggt
    12961 tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg gcactgtatt
    13021 gtttatcctg taagtacagt ttattcgttt gccgaacaat atttgccaga ttatttttag
    13081 ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg tcaccttgtc
    13141 gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact tgtgcagaaa
    13201 ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct tctaattcca
    13261 ttgaccaata ggcataggca cctgaaaacg caataaataa aactaccgcc aataaagaaa
    13321 agtattttct tctgttagaa tgttttttat ttgaattaat ctgttc
//
LOCUS       DAZFYC010000133        13311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFYC010000133 DAZFYC010000000
VERSION     DAZFYC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.133"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            244..579
                     /locus_tag="ACHKW5_004257"
     CDS             244..579
                     /locus_tag="ACHKW5_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO7513512.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            739..1182
                     /gene="yjdN"
                     /locus_tag="ACHKW5_004258"
     CDS             739..1182
                     /gene="yjdN"
                     /locus_tag="ACHKW5_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO7513513.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1315..2103
                     /gene="phnC"
                     /locus_tag="ACHKW5_004259"
     CDS             1315..2103
                     /gene="phnC"
                     /locus_tag="ACHKW5_004259"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO7513514.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2128..3144
                     /gene="phnD"
                     /locus_tag="ACHKW5_004260"
     CDS             2128..3144
                     /gene="phnD"
                     /locus_tag="ACHKW5_004260"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7513515.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3250..4029
                     /gene="phnE"
                     /locus_tag="ACHKW5_004261"
     CDS             3250..4029
                     /gene="phnE"
                     /locus_tag="ACHKW5_004261"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO7513516.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4050..4775
                     /gene="phnF"
                     /locus_tag="ACHKW5_004262"
     CDS             4050..4775
                     /gene="phnF"
                     /locus_tag="ACHKW5_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO7513517.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4776..5228
                     /gene="phnG"
                     /locus_tag="ACHKW5_004263"
     CDS             4776..5228
                     /gene="phnG"
                     /locus_tag="ACHKW5_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO7513518.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5225..5809
                     /gene="phnH"
                     /locus_tag="ACHKW5_004264"
     CDS             5225..5809
                     /gene="phnH"
                     /locus_tag="ACHKW5_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO7513519.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5809..6873
                     /gene="phnI"
                     /locus_tag="ACHKW5_004265"
     CDS             5809..6873
                     /gene="phnI"
                     /locus_tag="ACHKW5_004265"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO7513520.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6866..7711
                     /gene="phnJ"
                     /locus_tag="ACHKW5_004266"
     CDS             6866..7711
                     /gene="phnJ"
                     /locus_tag="ACHKW5_004266"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO7513521.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7708..8466
                     /gene="phnK"
                     /locus_tag="ACHKW5_004267"
     CDS             7708..8466
                     /gene="phnK"
                     /locus_tag="ACHKW5_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO7513522.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8577..9257
                     /gene="phnL"
                     /locus_tag="ACHKW5_004268"
     CDS             8577..9257
                     /gene="phnL"
                     /locus_tag="ACHKW5_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO7513523.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9254..10390
                     /gene="phnM"
                     /locus_tag="ACHKW5_004269"
     CDS             9254..10390
                     /gene="phnM"
                     /locus_tag="ACHKW5_004269"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO7513524.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAL
                     CRVRQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10390..10947
                     /gene="phnN"
                     /locus_tag="ACHKW5_004270"
     CDS             10390..10947
                     /gene="phnN"
                     /locus_tag="ACHKW5_004270"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO7513525.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10934..11368
                     /gene="phnO"
                     /locus_tag="ACHKW5_004271"
     CDS             10934..11368
                     /gene="phnO"
                     /locus_tag="ACHKW5_004271"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO7513526.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11370..12128
                     /gene="phnP"
                     /locus_tag="ACHKW5_004272"
     CDS             11370..12128
                     /gene="phnP"
                     /locus_tag="ACHKW5_004272"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO7513527.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTQKFLLNNHPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIHSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(12275..12604)
                     /gene="yjdP"
                     /locus_tag="ACHKW5_004273"
     CDS             complement(12275..12604)
                     /gene="yjdP"
                     /locus_tag="ACHKW5_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO7513528.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12673..13122)
                     /gene="rpiB"
                     /locus_tag="ACHKW5_004274"
                     /gene_synonym="alsI"
     CDS             complement(12673..13122)
                     /gene="rpiB"
                     /locus_tag="ACHKW5_004274"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO7513529.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQWRN"
BASE COUNT         2946 a         3998 c         3709 g         2658 t
ORIGIN      
        1 tcagaaagat aaaaatatca tgtgaataaa aaaagaacaa gtagagcatt aacattatct
       61 taaataataa atagaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac
      121 gatcgtcgtc taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg
      181 gcctttttcg gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc
      241 cacatgtcat taccacactg cccaaaatgc aactccgaat acacttacga agataacggc
      301 atgtacatct gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc
      361 gacgagctga tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc
      421 atcattaaag atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg
      481 aaaaacatcc gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt
      541 ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc
      601 ggataaggcg ctcgcgccgc atccggcaac gatgccgact gcctgatgcg acgctgaccg
      661 cgtcttatca ggcttctctc tcatctgtat aaatttcgaa ctacacttaa ctggcttctc
      721 ttaactgagg tcaccatcat gccgttaagt ccctacctct cttttgccgg taactgtgcc
      781 gacgcaattg cttattatca acgtacgttg ggcgctgaac tgctctataa aatcagcttc
      841 ggcgaaatgc caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt
      901 cccgataccg ccatcgccca cgccaacgtg cgcattgccg gaagcgacat catgatgagc
      961 gatgctatac cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa
     1021 caggtcgaag aaggaaaacg ctggtttgac aatcttgccg ctaacggaaa aatcgaaatg
     1081 gcctggcagg aaactttctg ggcgcatggc tttggcaaag tcaccgataa atttggcgta
     1141 ccgtggatga ttaatgtcgt caaacaacaa ccaacgcaat aacccgccgg gaggcccgcc
     1201 ctcccgcact gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc
     1261 aaatcgtcac aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa
     1321 acgattatcc gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc gctgcatgcg
     1381 gttgatctga acattcatca cggtgaaatg gtggctctgc ttgggccgtc gggttccggc
     1441 aaatccaccc ttttacgtca cttaagcggt ttgattaccg gcgataaatc cgccggcagc
     1501 catatcgagc tgctgggccg cacagtccag cgcgaaggcc gtctggcgcg cgatatccgc
     1561 aaaagccgcg ccaacaccgg ctacatcttc caacaattca acctggtgaa ccgcctgagc
     1621 gtactggaga acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt
     1681 agctggttca ccggcgagca gaaacagcgc gcgttacagg cgctgacccg cgttggcatg
     1741 gtgcattttg cccatcagcg cgtttccacc ctctccggcg gacagcagca gcgtgtggcg
     1801 attgcccgcg cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg
     1861 ctggacccgg aatccgcccg catcgtgatg gacaccctgc gcgacatcaa tcagaacgac
     1921 ggcatcaccg tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc
     1981 atcgtcgccc tgcgccaggg gcacgttttc tacgacggca gcagccaaca gtttgataac
     2041 gaacgttttg accatctcta ccgcagcatt aatcgcatcg aagagaacgc gaaagctgcc
     2101 tgacatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc
     2161 accagcatgt tcagcctcag caccctgtta agcccggcac acgccgaaga gcaggaaaag
     2221 gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg
     2281 ccattcttac aggatatgga gaagaagctg ggcgtgaagg tgaacgcctt ctttgcccca
     2341 gactacgcag gcattatcca gggaatgcgc ttcaataaag tggatatcgc ctggtacggc
     2401 aacctgtcgg caatggaagc ggtggatcgc gccaacggcc aggtcttcgc ccagacggtc
     2461 gcggcggatg gatcgccagg ttactggagc gtgttgatcg tcaacaaaga tagtccgatc
     2521 aacaacctga acgatctgct ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct
     2581 aactccacct ctggcttcct cgtccccggt tactacgtct ttgccaaaaa caatatctcc
     2641 gccagcgact ttaagcgcac cgtcaacgct gggcatgaaa ccaacgcgct ggccgtcgcc
     2701 aacaagcagg tggatgttgc caccaacaac accgaaaacc tcgacaagct gaaaacctcc
     2761 gcgccagaga agctgaaaga actgaaggtg atctggaagt cgccgctgat cccaggcgat
     2821 ccgatcgtct ggcgtaaaaa cctttccgaa accaccaaag acaagatcta cgacttcttt
     2881 atgaactacg gcaaaacgcc ggaagaaaaa gcggtgctgg aacgcctggg ctgggcgccg
     2941 ttccgcgctt ccagcgacct gcaactggtg ccgattcgcc agctcgcgct gtttaaagag
     3001 atgcagggcg tgaaaagcaa taaaggactg aatgagcagg acaagctggc aaaaaccacc
     3061 gagattcagg cgcagctgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt
     3121 tcggtgagta aagcggtgca gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc
     3181 gacaatgtgc aggcgttgat gccggatgcg gtgcaagcac cttatccggc ctacagaccg
     3241 gagccaaaca tgcaaaccat caccatcgcc ccacccaagc gcagctggtt ctcgcttctg
     3301 agctgggccg ttgttctcgc cgtgttggtc gtctcgtggc agggcgcgga aatggccccg
     3361 ctcacgctga ttaaagacgg cggcaacatg gcgacgttcg ccgccgactt cttcccgccc
     3421 gatttcagcc agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgtc
     3481 tggggcacgg cgctggcggt ggttctctcc atccccttcg gcctgatgag cgccgaaaac
     3541 ctggtgccgt ggtgggttta ccagcccgtt cgccgcctga tggacgcctg ccgcgccatt
     3601 aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg gcctcggccc gttcgctggc
     3661 gtgctggcgc tgtttatcca caccaccggc gtgctctcca aactgctttc cgaagcggtg
     3721 gaagcgattg aacccggccc ggtggaaggc attcgcgcca ccggcgccaa caagctcgaa
     3781 gagatcctct acggcgtgct gccgcaggtg atgccgctgc tgatctccta ctccctctat
     3841 cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca tggtcggtgc aggcgggatc
     3901 ggcgtcaccc tgtgggaagc gattcgcggt ttccagttcc aacaaacctg cgccctgatg
     3961 gtgcttatca tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac
     4021 tttatctgat aagcgaggca ttgatatcta tgcacttgtc tacacatccg accagctacc
     4081 caacacgcta tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactaccgct
     4141 gcggcgacta tcttcccgcc gagcagcaac tggcggcgcg ctttgaggtg aaccgccaca
     4201 ccctgcgccg cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg
     4261 tcggcgtgct ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta
     4321 gccagaacct gctggaccag ggtagccatc ccaccagcga aaaactgctg tcggtattgc
     4381 gccccgcgtc cggtcacgtc gctgacgcgc tggggattac cgagggcgag aacgttatcc
     4441 atttgcgcac cctgcgtcgc gtcaacggcg tcgcgctctg tttaatcgac cactactttg
     4501 cggacctcac cctttggccg acgctgcaac gcttcgacag cggttcgctg cacgattttc
     4561 tgcgcgagca aaccggaatt gcgctgcgcc gcagccagac gcggatcagc gcccgtcgcg
     4621 cccaggccaa agagtgccag cgtcttgaaa ttccgaatat gtcgccgctg ctgtgcgtgc
     4681 gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc
     4741 gcgccgacat gattgaattc actatggagc actgaatgca cgcagatacc gcgacccgcc
     4801 agcactggat gtccgtactg gcgcacagcc aaccggctga actggcagcc cgcctgaaag
     4861 cactgaacat caccgccgac tatgaggtta tccgcgccgc cgaaaccggc ctggtgcaga
     4921 ttcaggcgcg gatgggcggc accggcgaac gcttttttgc cggcgacgcc acgctcaccc
     4981 gcgccgccgt acgcctgact gacggcacgc tcggctacag ctgggtgctg gggcgcgaca
     5041 aacagcacgc cgaacgctgc gcgctgattg acgcgctgat gcagcaatct cgctattttc
     5101 aaaacttatc agaaaccctt attgccccgc tggacgctga ccgtatggca cgcattgccg
     5161 cacgccaggc cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagaca
     5221 acgcatgacc ctggaaaccg cttttatgct tcccgtgcag gatgcccagc acagttttcg
     5281 tcgcctgtta aaggccatga gcgagccggg cgtgattgtc gccctgcatc agctcaagcg
     5341 cggctggcag ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga
     5401 cacgccggtg tggcttgccg cgccattgag taacgatatc gtcagccaga gcctgcgttt
     5461 tcataccaac gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg tgacggatga
     5521 ggcgatttcc agcgaacagc ttaacgccct ttccaccggc accgccgttg cgccggaagc
     5581 gggtgcaacg ctgattttac aggtcgccag cctgagcggc gggcgcatgt tacgtctcac
     5641 cggcgcgggc attgccgaag aacgaatgat cgctccgcag ctgccggagt gcattctgca
     5701 cgaactcacc gagcgcccgc acccgttccc gctcggcatc gacctgatcc tgacctgcgg
     5761 cgagcgcctg ctggctattc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc
     5821 gtgaaagggg gcgagaaggc gatcgacgcc gctcacgccc tgcaagagag ccgacgccgg
     5881 ggcgataccg atttgcctga actgagcgtc gcccagattg aacagcagct taatcttgcg
     5941 gtggatcgcg tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg
     6001 aaacaggcca gcggcgataa cgtcgaagca attttcctgc tgcgcgccta ccgcaccacg
     6061 ctggcgaagc tcgcggtaag cgagccgctc gacaccacag agatgcgtct cgaacggcgt
     6121 atttcggcgg tttataaaga cattcccggc ggccagctgc ttggcccaac ctacgactac
     6181 acccatcgcc tgctcgattt caccctgctg gcaaacggtg aagcgccgac gctgaccacc
     6241 gccgacagcg aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg
     6301 gcgaagtttg aagaggatag cggcgcacag ccggatgata tcacccgcac gccgccggtt
     6361 tacccctgct cacgttcttc ccgtttgcag cagttgatgc gcggcgacga aggctatttg
     6421 ctggcgctgg cctactccac ccaacgcggt tacgggcgca atcacccgtt cgcgggcgag
     6481 atccgcagtg gttacatcga cgtgtcgatt gtgccggaag agctgggatt tgcggtgaac
     6541 gtcggcgaac tgctgatgac cgaatgtgaa atggtcaacg gttttatcga cccgccggat
     6601 gagccgccgc acttcacgcg cggctacggg ctggtattcg gcatgagcga gcgcaaagcg
     6661 atggcaatgg cgctggttga ccgtgcgttg caagccccgg aatacggcga gcacgcgaca
     6721 ggcccggcgc aggatgaaga gttcgtgctg gcgcatgccg acaacgtcga agccgcaggc
     6781 tttgtctcgc acctcaaact cccgcactac gtcgatttcc aggccgaact ggagctactc
     6841 aaacgtctgc aacaggagca gaaccatggc taatctgagc ggctacaact ttgcctacct
     6901 cgacgagcag accaaacgca tgatccgccg cgccatctta aaagcggtgg ctatccccgg
     6961 ttatcaggtg ccgtttggcg ggcgcgagat gccgatgccc tacggctggg gaaccggcgg
     7021 cattcagctc accgccagcg tgattggcga aagcgacgtg ttgaaggtga ttgaccaggg
     7081 tgcggatgac accaccaacg ccgtgtcgat tcgcaacttc tttaagcgcg tgaccggggt
     7141 aaacaccact gaacgtacgg acgatgcgac gcttatccag acgcgtcacc gcatccccga
     7201 aacgccgctg accgaagatc agatcattat cttccaggtg ccaatcccgg aaccgctgcg
     7261 ctttatcgag ccgcgcgaaa cggagacccg cactatgcac gcgctggagg agtacggcgt
     7321 gatgcaggtg aaactgtatg aagatatcgc ccgcttcggt catatcgcca ccacctatgc
     7381 ttatccggtg aaggtgaacg ggcgctacgt gatggacccg tcgccgatcc cgaaattcga
     7441 taacccgaaa atggacatga tgcccgccct gcagctgttc ggagcagggc gcgagaagcg
     7501 catctatgcg gtgccgccgt ttacccgcgt ggaaagtctc gatttcgacg atcacccgtt
     7561 caccgttcag cagtgggatg agccatgcgc catctgcgga tcgacccaca gctatcttga
     7621 tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg
     7681 ccgccaacag agcgaggcga aaagccaatg aataaaccgt tactttcggt caataacctg
     7741 acccaccttt acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccggga
     7801 gaagtgctgg gcattgtcgg agaatccggc tccgggaaga ccacgctgct gaagtcgatc
     7861 tccgcgcgtc tgacgccgca gcagggggag attcactacg agaaccgttc gctgtatgca
     7921 atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtagt gcatcagcat
     7981 ccactcgacg gcctgcgccg tcaggtctcg gcaggcggca atatcggcga gcggttgatg
     8041 gcgaccgggg cgcgtcatta cggcgacatt cgcgccaccg cgcagaagtg gctggaagag
     8101 gtggagattc ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggtatgcag
     8161 cagcgtttgc agattgcccg caacctggtg acgcatccga agctggtgtt tatggatgaa
     8221 ccgaccggcg ggctggatgt gtcggtgcag gcccgcctgc tcgacctgct gcgcggcctg
     8281 gtggtggagc tgaacctcgc ggtggtgatt gtcacccatg atttaggcgt cgcccgcctg
     8341 ctggcggacc gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac
     8401 cgcgtgctcg acgacccgca tcatccgtat acacagctgc tggtgtcatc ggttttgcag
     8461 aattgagccg gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg
     8521 taggtcggat aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga
     8581 ttaacgtaca aaacgtcagt aaaaccttca tcctgcacca gcaaaacggc gtgcgcctgc
     8641 ccgtcctcaa tcgcgcctcg ctcaccgtca acgcgggcga atgcgtggtg ctccacggcc
     8701 attccggcag cggcaaatca actctgctac gctcgctgta cgccaactat ctacccgacg
     8761 aaggtcaaat ccagatcaaa cacggtgacg agtgggtaga cctggtcacc gcgccagcgc
     8821 gcaaagtggt ggaaatccgc aaaaccaccg tcggctgggt gagccagttt ctgcgcgtca
     8881 tcccgcgtat ctcagcactg gaagtggtga tgcagccgct gctcgatacc ggcgttccgc
     8941 gtgaagcctg cgccgctaaa gccgcgcgtc ttctcacccg cctgaacgtg ccggaacgcc
     9001 tgtggcacct ggcaccatcg acattttccg gtggcgaaca gcagcgcgtc aacatcgccc
     9061 gcggctttat cgtcgactac cccattctgc tgcttgacga acctaccgcc tcgctggacg
     9121 ccaaaaacag cgccgcggtg gtggaactga ttcgcgaagc caaaacccgt ggcgcagcca
     9181 tcgtaggcat cttccatgac gaagctgtac gtaatgacgt cgccgaccgc ctgcacccaa
     9241 tgggagcctc ttcatgatta tcaataacgt taagctggtg ctggaaaacg aggtggtgca
     9301 cggttcgctg gaggtgcagg atggcgaaat ccgcgccttt gccgaaagcc agagccgcct
     9361 gccagaagcg atggacggcg aaggtggctg gctactaccc ggcctgattg agctgcatac
     9421 cgacaacctc gataaattct tcaccccgcg cccgaaagtc gactggcccg cccattcggc
     9481 gatgagcagc cacgacgcgc tgatggtggc gagcggcatc accaccgtac tggatgccgt
     9541 agcaattggc gacgtgcgcg acggcggcga tcggctggaa aatctggaga agatgatcaa
     9601 cgccatcgaa gagacgcaga aacgcggcgt caaccgcgcc gagcaccgcc tgcatctgcg
     9661 ctgcgaactg ccgcatcaca ctacgctgcc gctgtttgaa aaactggtgc agcgcgagcc
     9721 ggtgacgctg gtgtcgctga tggatcactc gccgggccag cgccagttcg ccaaccgcga
     9781 gaagtatcgc gagtattatc agggcaaata ctcgctcacc gatgcgcaga tgcagcagta
     9841 cgaagaagag caactggcgc tcgccgcacg ctggtcgcag ccgaatcgcg aaactatcgc
     9901 cgccctgtgc cgcgtaagac aaattgccct cgccagccac gatgacgcca cgcacgccca
     9961 cgttgccgaa tcccaccagc ttggcagcgt gatcgccgaa tttcccacta cgttcgaagc
    10021 agcggaagcc tcgcgcaagc atggcatgaa cgtgctgatg ggcgcgccaa atatcgtgcg
    10081 tggcggttcg cactccggca acgtggcggc cagtgaactg gcgcagcttg gcctgctgga
    10141 tatcctctct tccgactact accccgccag cctgctggat gcggcgttcc gcgtcgccga
    10201 tgacgagagc aaccgcttta cgctgccgca ggcggtgaag ctggtgacca aaaatccggc
    10261 gcaagcgctt aatctccagg atcgcggggt gattggcgag ggtaaacgcg ccgacctggt
    10321 gctggcgcat cgcaagggca accacattca tatcgaccac gtctggcgtc agggtaaaag
    10381 ggtgttctga tgatgggaaa actgatttgg ttaatggggc cgtccggctc cgggaaagat
    10441 agcctgctgg cggaactccg cctgcgggaa caaactcagc tactggtggc gcatcgctac
    10501 atcacgcgcg acgccagcgc tggaagcgaa aaccatatcg ccctgagcga gcaggagttt
    10561 tttacccgcg cggggcaaaa tctgctggcc ttaagctggc acgctaacgg tctgtattat
    10621 ggcgtcggcg tcgagattga cctctggctg cacgctggat tcgacgtgct ggtcaacggc
    10681 tcacgcgccc atctgccgca ggcgcgggcg cgctatcaat cggcgctgct gcccatttgc
    10741 ttacaggttt cgccagagat cctgcgccag cgcctggaaa accgtggccg cgaaaacgcc
    10801 agtgaaatta acgcccggct ggcgcgcgcc gcccgctata ctccacagga ttgccatacg
    10861 ctcaataatg acggcagcct gcgccagtcg gtcgacacgc tgctgacgct aatccatcag
    10921 aaggagaaac accatgcctg cttgtgagct tcgcccggcc acgcagtacg acaccgacgc
    10981 ggtttacgcg ttgatttgcg agctaaaaca ggcggagttt gaccaccacg cgtttcgcgt
    11041 gggctttaac gccaacctgc gcgacccgaa catgcgctac catctggcgc tgctggatgg
    11101 cgaagttgtc ggtatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaactggat
    11161 cggcgaaatt caggagttgg tggtaatgcc gcaggcgcgc ggtctgaacg tcggcagtaa
    11221 gttactggcg tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgaactttc
    11281 gaccaacgtg aagcgccacg acgcgcaccg tttctatctg cgagaaggct acgagcagag
    11341 tcacttccgc ttcaccaagg cgctgtaaca tgagtctgac cctcacgctc accggcaccg
    11401 gcggcgcaca gggcgttccg gcatggggct gcgagtgcgc ggcctgcgac cgagcgcggc
    11461 gctcaccgca gtatcgccgc cagccatgca gcggcgtagt gaagtttaac gacgcaatca
    11521 ccctgatcga cgccgggctg cacgatctcg ccgatcgctg gtcgcccgga tcgttccagc
    11581 agtttttgct gacgcattat catatggatc acgtccaggg gctgtttccg ctgcgctggg
    11641 gcgttggcga tccgatcccg gtttacggcc cgccggatga ccagggttgc gacgatctgt
    11701 ttaaacatcc gggcctgctt gatttcagcc acacagtgga accgtttgtg gtatttgatt
    11761 tgcaggggtt gcaggtcacg ccgctgccgc ttaaccactc aaaactgacc ttcggttatc
    11821 tgctggaaac ggcacatagc cgggtggcgt ggctatctga caccgctggc ctgccggaaa
    11881 aaacacagaa atttttactc aataatcatc cgcaggtgat ggtgattgat tgcagccacc
    11941 cgccgcgcga ggatgcgccg cgtaatcact gtgatttaaa taccgtgctt gcgctcaatc
    12001 aggttatcca ctcgccacgg gtgattctga cccatatcag ccaccagttt gatgcgtggc
    12061 tgatggagaa tgcgctgccg ccgggttttg aggcggggtt tgatgggatg gagattgggg
    12121 tggcgtgatg agagggaatg tgcgcgctgg ccccctcacc ctaaccctct ccccagaggg
    12181 gcgaggggac cgattgtgct cgatattgaa tattgcgctg gttttctccc tctccccatt
    12241 ggggtgaggg gcgatgcctg ctccataccc aacctcatcg cccatactca tcttccattc
    12301 tccgctcttc atcttctagc tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca
    12361 gctgcctgcg gcgatcttca aactggcggc ggcggtcgtc atattgtctg cgccgatcgt
    12421 cgctcacttc acgctgccag ccgtcgtcgc gcgaatcttc atagtctcgc ccacggtcag
    12481 ggttataagc gtcgttaatc gcctgctgaa tattgccaat ggtgtcgtcg ataatatcgg
    12541 cctgggccgg aacggtggac agcgtgagca gggtgaataa aagaaatagc ggaaagcgtt
    12601 tcattagcca acctcaaaaa gaaactctat ccacattagt cattactcat ccatgcaagt
    12661 agtggatgaa tctcaatttc tccactgctc tattgccgta atcgcctcca cgcgttgttg
    12721 atgacgaccg ccttcgtact gtgcgcccag ccacgcatcc acaatcattt ttgccagttc
    12781 gaggccaacc actcgtgaac caaaagccag cacgttggtg tcgttatgct gccgcgaaag
    12841 ttgcgcggaa taaggttcgc tacagacgac cgcgcgaatt ccggcaaact tgttcgccgc
    12901 tatcgaaata ccgacgccag taccacaaat caaaatcccg ccatcaacct ctccgccagc
    12961 aacagccagt gcgacttgac tggcgtaatg tggataatca gtacgctctg acgaccaggt
    13021 tcctttatca atcacttcaa cgccacgctc aactaaatgt gccactattt catgttttaa
    13081 aatgaaaccg acatgatcac agccaaatgc aatctttttc attcatcttc cccgtccgtg
    13141 caaaacttca caatcaaaaa acatttgtga tgttaatgaa ttaaaaacac ccaaaatcga
    13201 tgaattacgc cttcctgatc tgttttacag agttacatct gacagcgcaa tagcgttaaa
    13261 gacactcacc atccagcaac atctatcatc taaaaaacca gaaaaacaaa t
//
LOCUS       DAZFYC010000134        13175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFYC010000134 DAZFYC010000000
VERSION     DAZFYC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.134"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(268..576)
                     /locus_tag="ACHKW5_004275"
                     /pseudo
     CDS             complement(268..576)
                     /locus_tag="ACHKW5_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(586..1283)
                     /locus_tag="ACHKW5_004276"
     CDS             complement(join(586..1034,1034..1283))
                     /locus_tag="ACHKW5_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X3 family transposase"
                     /protein_id="HFO7513530.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYGRMCRTVVARVFGERTLATLERLPGLLSAFEV
                     VVWMTDGWPLYESRLKGELHVISKRYTQRIERHNQNLRQHLARLGRKSLSFSKSVALH
                     DKVIGHYLNIKHYQ"
     gene            complement(1435..2922)
                     /gene="ltrA"
                     /locus_tag="ACHKW5_004277"
     CDS             complement(1435..2922)
                     /gene="ltrA"
                     /locus_tag="ACHKW5_004277"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO7513531.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRR
                     TQSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRW
                     MDYAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALV
                     KILNAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHE
                     WLIRFTEHRIGDRSVIRLIRKWLTAGTLEEGQWRATEEGTPQGAVISPLLANIYLHYV
                     FDLWAHQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEK
                     TRLMEFGRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLK
                     AIKDGLRRRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRR
                     RSQKDDTTWTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
     gene            3328..3759
                     /gene="umuD"
                     /locus_tag="ACHKW5_004278"
     CDS             3328..3759
                     /gene="umuD"
                     /locus_tag="ACHKW5_004278"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7513532.1"
                     /translation="MKLQLFSTGKELPPQAHPLFADLASCGFPSPAADYVESDLDLHD
                     YCIRHPSATYYLRASGDSMADGSLYNGDLLVVDSAEKPRHGDIVVASMQGEFTVKRLL
                     LTPRLTLQPMNASWSPIYPDPDELDIFGVVTHIIHRPREMY"
     gene            3759..5030
                     /locus_tag="ACHKW5_004279"
     CDS             3759..5030
                     /locus_tag="ACHKW5_004279"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO7513533.1"
                     /translation="MFALADVNSFYASCERVFRPDLKGKPIVVLSNNDGNVIARSAEA
                     KPWIKMGTPWFQIKNERYPEKIYAFSSNYELYASISARVMNCLEELAPRVEQYSIDEM
                     FLDLTGVEHCMGLEDFARQLRQQVYDCTRLTIGVGAGPTKTLAKSAQWASKEWKQFHG
                     VLALTRGNPQRTRKLLSLQPVEEIWGVGNRIARKLNVLGIKTALDLALTNPAFIRKNF
                     SVVLERTVRELNGESCLSLEEAPPTKQQIVCSRSFGVKITEYESLRQAICQHAERASE
                     KLRKEHQYCRHISVFIKTSPFAIKEPYYGNVATEKLLTPTQDTRDIIAAATTALERIW
                     RDGHRYAKAGVMLNDFTGSGVSQLQLFDERPPRPHSAELMRVLDGINNSGLGKVWFAG
                     RGMAPEWQMKREMLSPAYTTRWSDIPAAKLA"
     gene            complement(5112..6086)
                     /locus_tag="ACHKW5_004280"
     CDS             complement(5112..6086)
                     /locus_tag="ACHKW5_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO7513534.1"
                     /translation="MSNEKRKTIGRQLNTQASMLEMADTGRSQVFTLKTGRKVTFRFV
                     RVPASEVESKTFVNQENNGRDQLALTRESLKSIIQTIKFQQFFPCIGIKQSERIEILD
                     GSRRRASAIFVRTGLDVMVTEEHLSADEARQLAKDIQTAKEHNLREIGLRLIALKESG
                     FNQKEIAELEGLSQAKVTRALQAAAVPQDLISLFPVQSELSFSDYKLLLEVNEKLGEK
                     GLTPEELIQSVSAQLDAILSDGERPEDEQKASILKLISQASQALIDPSPKEKSVVSPL
                     WSFEEKDKFARKRVKGRTLTYEFSRMSKVIQDELDKAINEVLDRNLSQ"
     gene            complement(6086..7291)
                     /locus_tag="ACHKW5_004281"
     CDS             complement(6086..7291)
                     /locus_tag="ACHKW5_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7513535.1"
                     /translation="MDNIEQLRKVATRAGKLLTSLSESIRQQKEELKLTEFYQEYSKA
                     ALYKLPKLSKGSVEYAVSEMEAGGYIFKKKPSGNTMKYAMTIQNVIDLYNHRKVPKYR
                     DRFDKAFTIFVCNLKGGGSKTVSTASLSHAFRAHPQLLFEDLRILAIDFDPQASLTMF
                     LSHENSVGLVENTAAQAMLQNVSREELLSDFIVPSIIPGVDVIPASIDDAFLAEGWKG
                     LCEEHLPGKNIHAVLKENIIDKLQHDYDFIFLDSGPHLDAFLKNCIGAADLMLTPLPP
                     ATVDFHSSLKFVASLPALIDSIEMDGHTCNLIGNVGFMSKILNKSDHKICHSQAKEVF
                     GADMLDMVLPRLDGFERCGETFDTVISANPATYDGSTEALKSAKSAAEDFAKAVFDRI
                     EFIRTNGGM"
     gene            7706..7975
                     /locus_tag="ACHKW5_004282"
     CDS             7706..7975
                     /locus_tag="ACHKW5_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513536.1"
                     /translation="MDAPDYGKQVNFADGPDYGKQVNFTGGRECGEQVNFADGPDYGK
                     QVNFAGGRDCGKQVNFADGPDCGKQVNFAGGRECGEQVNFADGPD"
     gene            complement(8152..9018)
                     /locus_tag="ACHKW5_004283"
     CDS             complement(8152..9018)
                     /locus_tag="ACHKW5_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO7513537.1"
                     /translation="MGKRSSSVLNYPLVDKVQIFPVDKYRDSLQFLFSLSLWVGKNLP
                     IGIEMDTQALLPATKTFKKRASIKQSNELTEAAYYLPLQAKRVLWLCLMQAYFNDSQE
                     DDSDVLPLFKISVSDYVKYFNVATSVASRDVKAGVNALGESTVTFYPKEGEFEEVKRP
                     WLAEAGMKRGRGSWQIEFNYKVMPFLVGLTSQFTTYSLYDCGQLNSVRVIRLYESLCQ
                     FRSTGVWITTHDWLCERFMLPASQKNNIAEMKRTFLEPALKKINEKTPLKVTYTTEED
                     GRLLFNFLDKKQ"
     gene            9548..9652
                     /locus_tag="ACHKW5_004284"
     CDS             9548..9652
                     /locus_tag="ACHKW5_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513538.1"
                     /translation="MRSFTIMSLLETFLTASQGATILFLAMLIYWQKR"
     gene            9781..10038
                     /locus_tag="ACHKW5_004285"
     CDS             9781..10038
                     /locus_tag="ACHKW5_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513539.1"
                     /translation="MKLETIQIDNTVRSGLNSFTDGVIIRDTKVLITLNLPDGQKTTI
                     EGEWRHMSDANRLSERITMLQHAIYEQYEEYRADIPVSWSR"
     gene            complement(10096..10872)
                     /locus_tag="ACHKW5_004286"
     CDS             complement(10096..10872)
                     /locus_tag="ACHKW5_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7513540.1"
                     /translation="MSELISWNQGTPPAQLPVAIDYSAALALRQMAVVQDELPKYLLA
                     PEVSALLHYVPDLHRKMLLATLWNTGARINEVLALTRGDFSLAPPYPFVQLATLKQRT
                     EKAARTAGRAPAGSQAHRLVPLSDHHYVSQLQMMVATLKIPLERRNKRTGKVEKARIW
                     EITDRTVRTWLSEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMGH
                     KSISSTEVYTKVFALDVAARHRVQFQMPEADAVAMLKGNI"
     gene            complement(10884..11570)
                     /locus_tag="ACHKW5_004287"
     CDS             complement(10884..11570)
                     /locus_tag="ACHKW5_004287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513541.1"
                     /translation="MADGISVWIPVITALAGIGGALGSQYISHRFTLIREKKASEDKM
                     QRERYFIATRLVFLLERFAQRCVYSAYESGFNEPEHGHFRVNHTLPELSYDGIDGDWR
                     SLPPELMFRLSQMPVLQQEAKQSIESAFGNDNPYDGSNGLSEINKQSSRLGLRAIRLS
                     RELRQICSMPHDDLSAHHWSAWRMLSIARARSINAELCYARSQHKYHASLRLMESVDS
                     LESTGLPDEE"
     gene            complement(11634..11927)
                     /locus_tag="ACHKW5_004288"
     CDS             complement(11634..11927)
                     /locus_tag="ACHKW5_004288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513542.1"
                     /translation="MVKRKQVDIHEIYEALKCLGHTRLWLRDNGKYNRLIITWDNLVA
                     ASYDPYLKSWNFRKNACRVNYNAKNEIPPGKSSVLTELADSGHAWMQMLDGDW"
     gene            12516..12746
                     /gene="vapB"
                     /locus_tag="ACHKW5_004289"
     CDS             12516..12746
                     /gene="vapB"
                     /locus_tag="ACHKW5_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO7513543.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVR
                     PTWGSFAQLDRADPDFMAEREDVVSDEGRFEP"
     gene            12743..13159
                     /locus_tag="ACHKW5_004290"
     CDS             12743..13159
                     /locus_tag="ACHKW5_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO7513544.1"
                     /translation="MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTY
                     AEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRAAVDATTDIRVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK"
BASE COUNT         3139 a         3468 c         3356 g         3212 t
ORIGIN      
        1 cgggcgccgg tgctgtgtgg aaatccgccc accttaccgg gcgccggtgc tgtgtggaaa
       61 tccgcccatc ttgccgggcg ccggtgccgt gtgggaatcc gcccacctta ccgggcgccg
      121 gtgccgtgtg ggaatccgcc caccttaccg ggcgccggtg ccgtgtggga atccgcccac
      181 cttaccgggc gccggtgctg tgtggaaatc cgcccatctt catgacaaaa atttgtcaaa
      241 tgacaggctc atgtgtgctg atttatgtca gacgggattt tgcttatcaa ttattgagaa
      301 cagaaatttt cctgaatcat caatactata cttagcaagt aaaggtgttt tctcatttat
      361 ctgcttgaga gcaggatcaa ggaaagatcg tttcaactct gccaagttct tctgttggga
      421 ttccggcaaa aggaaacggt catttaacca agcatgagta gtaacccata agcctgaaga
      481 tttgaattga gcaagacttt cataaaggcg gatcgtccgg ggatttcgaa gactgccaca
      541 atcgcggagc gagtaggtgg tgaactggtt ggtaatgact ccaacttact gatagtgttt
      601 tatgttcaga taatgcccga tgactttgtc atgcagcgcc accgattttg agaacgacag
      661 tgacttcctg cccagccttg ccagatgctg cctcagattc tggttatgcc gctcaattcg
      721 ctgcgtatat cgcttgctga taacgtgcag ttctcccttc aggcgggatt cataaagcgg
      781 ccagccatcc gtcatccata ccacgacctc aaaggccgac agcaggcccg gaagacgctc
      841 cagcgtggcc agagtacgct caccaaagac gcgtgccacc accgtcctgc acatcctgcc
      901 ataggcgtaa aataaccagc gctgacgtga tttagccccg acgtagcccc actgttcgtc
      961 catttccgcg cagacaatga cgtcactgcc cggttgtatg cgtgaggtta ccgactgcgg
     1021 tctgagtttt ttaagtgacg taaaatcgtg ttgaggccaa cgcccataat gcgggcggtt
     1081 gcccggcatc caacgccatt catggccata tcaatgattt tctggtgcgt accgggctga
     1141 gaggcggtgt aagtgaactg tagctgccat gttttacggc agtgagagca gagatagcgc
     1201 tgatgtccgg cagtactttt accgttacgc accacgcctt cagtagctga gcaggaggga
     1261 caactgatgg agatggaagc cactggagca cctcaaagac accatcgtac actaaatcag
     1321 taagttggca ccattatccg gcaggcgatc tggattatcg tcacgctgtt aaatatgtcg
     1381 cggtagggat acccgttacc ggataccccc cgcacagatc ccggcgtgcg cgatttacgc
     1441 accgggctcc tgcctcgggt gtctggcggt gaaccgctcc acaggccatg gatgaagaac
     1501 ccgaaccctt ggtagccatg cggctgccag tttgtttgct ttcgtccagg tcgtatcatc
     1561 cttctggctc ctgcgcctga gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt
     1621 ctgcatggtg gggaagttgc ccggtaccga gtgatagttc aggtatccct gaaccactct
     1681 cctgagccat tttccctgtt cggggattga gtaatgccag cgccttcgca gaccgtcttt
     1741 gatggctttc agagttgccg tcatccgatc ccggcgggtc tttcgtatca gcatgaacct
     1801 gccgttgcga tctttcccgc tgatgtgcgt gaacccgagg aagttgaacg tttctggttt
     1861 gccttttccc ctgatggcac ggttttcggc agcgaagcgg ccgaactcca tcagacgggt
     1921 tttctccggg tgaaccgtga gtccgaactc cctcagtctg cgctgcatgg ctatacggaa
     1981 gcgccgggca tcgtatcgtt tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat
     2041 taccacattg cctgtggcat agcgacgtcg ccactgatgc gcccacagat cgaagacgta
     2101 gtggaggtat atgtttgcca gcagcggtga gatgaccgca ccctgtgggg tgccttcctc
     2161 cgttgctcgc cattgaccct cctccaacgt cccggctgtg agccacttac gtatgagcct
     2221 gattacgctc cggtcgccga tccgatgctc tgtgaacctg atcagccatt cgtggctcac
     2281 cctgtcgaag aactgactga tgtcggcatc cagtacccag tttacgttag tgcgtaccag
     2341 ccctgtggcc agtgcgtcca gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga
     2401 gaaccccata aagtcgtttt catagactgc gttcaggatt ttcaccagcg catactggac
     2461 gatcttgtcc tccagcgagg cgatgccgag cgggcgttgt tttccatccg cttttgggat
     2521 gtagtgacgc ctgccgggct gcgccctgta gctgccctga tgtagcctcc ggtgcagatc
     2581 tgttatgttg ttcttcatgt ttccggcgta gtccatccac ctgatgccat ccactccggc
     2641 ggccgctttc ctgctcaggg agaggaatgc ggcttccagt gcttcgactg tcagcaggtg
     2701 gaacaatgct gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag
     2761 cctctgtgac atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc
     2821 cttggccccg ctccttcgct ccactgactc cgctcctttc gggttgttcg cctgcttcgc
     2881 cgctactatg agcgagtccg acttctcctc tccgtacatc accggctatg actcctcgtc
     2941 ttcccggtgc gggccgtctc cgacactggc agatggtcag aggggagatc tcccggttcc
     3001 cgcgtagaga tcgtattgac atgccagggt ctcagacccc gccgggtcca tgtggcactc
     3061 gcagtatcgc accctatgat gttgccttcc gttaacagta caacgtcggc acccggtaat
     3121 ttaatataca tttcgtggct caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc
     3181 catacctcgc ggtatgcaac gcatgactcg gggaccttgt ggattgctgg tccttcaatg
     3241 gtcggggact ttcacccctt gatctctacc ggtctcccgg cgaacactgt atgtataacc
     3301 agtaaaatta gagtgtcagg aggaaagatg aaactacaac ttttcagcac cgggaaagaa
     3361 ctcccgccac aggcccatcc attgtttgcc gatctggcca gctgcggttt tcccagccct
     3421 gctgctgact atgtcgagtc cgatctggat ctccatgatt actgcatacg gcatccgagc
     3481 gcgacgtact acctgcgtgc cagcggagac agcatggccg acggcagtct gtacaatggc
     3541 gatctgctgg ttgtggacag tgctgaaaag ccgcggcacg gcgatatcgt tgtcgccagc
     3601 atgcaggggg agtttacggt gaaacggctt ctgctgacgc cgcgactgac gctgcagccc
     3661 atgaatgcct cctggtcgcc gatttatcct gatcccgacg agctggatat ctttggcgtc
     3721 gtcacgcata tcatccaccg cccgagggag atgtactgat gtttgccctg gccgatgtga
     3781 acagcttcta tgccagctgt gaacgtgttt tccgcccgga tttgaaagga aagccgatag
     3841 tcgtcctgag caacaacgac gggaatgtaa tcgctcgcag tgccgaggcg aagccctgga
     3901 tcaaaatggg aactccgtgg tttcagataa aaaacgaaag atacccggaa aaaatatatg
     3961 cgttttcaag taattatgaa ctttacgcct cgatatcggc gcgcgtgatg aactgcctgg
     4021 aggaactggc ccccagggtg gaacagtatt caattgatga gatgttcctc gacctgaccg
     4081 gcgtggagca ctgtatgggg cttgaggact tcgcccggca gctgcgtcag caggtgtatg
     4141 actgtacccg cctgaccatt ggcgtgggcg ccggaccaac gaaaaccctc gcaaaatcag
     4201 cccagtgggc ctcaaaagag tggaaacagt ttcatggcgt actcgctctg accaggggaa
     4261 atccgcaaag gacgcggaaa ctgctttcac tgcagccggt tgaagaaatc tggggggtgg
     4321 gtaaccgtat tgcacgcaag ctaaatgtgc tgggcataaa aaccgcgctc gatctggctc
     4381 tgacgaaccc ggccttcatc cgcaaaaatt tttctgtcgt ccttgagcga acggtacggg
     4441 aactgaacgg ggaaagctgc ctttcgcttg aggaagcccc tccgacgaaa cagcaaattg
     4501 tctgcagccg cagttttggc gtgaagatca cggagtacga gtcactccgt caggctatct
     4561 gtcagcacgc agagcgggcc tctgaaaaac tgcgtaaaga gcaccagtat tgccggcata
     4621 tttccgtttt catcaaaacg tctccgttcg ccattaaaga gccttactac ggcaacgtgg
     4681 ctacggaaaa acttcttaca cccactcagg acaccaggga catcattgca gccgctacaa
     4741 cggctctgga gcgtatctgg agggatgggc accggtatgc caaagccgga gttatgctga
     4801 atgacttcac cggctccgga gtttcgcagc tgcaactgtt cgacgaacgc ccgccacgcc
     4861 ctcacagtgc tgagctgatg agggttctgg acgggatcaa caactcaggg ctgggtaaag
     4921 tatggtttgc ggggcggggt atggccccgg aatggcagat gaaacgggag atgctttctc
     4981 ctgcttatac gacacgatgg tcagatattc ctgcagcgaa actggcgtga aactatggcg
     5041 atttcaccgt gaaattctca acctaaactg cctaattaca ataagttaga tgtgaaatcg
     5101 ccatggcgaa attattgact caaatttctg tcaaggactt cgttgatagc cttgtccagt
     5161 tcatcctgaa tcacttttga catacggcta aactcataag tcagcgtgcg ccctttcaca
     5221 cgcttacgcg cgaacttgtc cttttcttca aaagaccaga gtggggaaac cactgacttt
     5281 tccttgggag atggatctat caatgcctga gatgcctgag aaatcagctt cagaatactg
     5341 gctttctgtt cgtcttctgg tcgctcgcca tcactcaaaa ttgcatcgag ctgagctgat
     5401 acagactgaa tgagttcttc aggcgttaat cccttttcac cgagtttttc attaacttct
     5461 aaaagaagtt tataatcact aaacgatagc tccgactgaa ccgggaacag agaaattaag
     5521 tcttgcggta cggccgccgc ctgcagcgcc ctggtaactt tagcctgaga taatccctcc
     5581 agttcagcaa tttccttttg attaaatccg gactctttaa gagcgatcaa tctcagacca
     5641 atctcccgaa gattatgttc tttagctgtc tggatatctt tagccagttg gcgagcttca
     5701 tcagctgata aatgttcttc ggtaaccatt acatcaaggc ctgtacggac aaaaattgct
     5761 gaggctcgcc gtcttgaacc atccagtatc tcaatccttt cactttgctt aataccaata
     5821 cagggaaaga actgctgaaa cttaatcgtc tggataatgg atttcagaga ctccctggtc
     5881 agtgcaagtt gatctcttcc attgttttcc tggtttacga aggtcttact ttcaacttca
     5941 gatgcaggca cccgaacaaa cctgaacgtt accttcctgc cggtttttaa ggtaaatacc
     6001 tgacttctac cggtgtcagc catttcgagc attgacgcct gagtatttaa ctgtctgccg
     6061 atggtttttc ttttctcatt cgacatcaca tacctccgtt agtacgaatg aactcgatgc
     6121 gatcaaagac tgccttcgca aagtcttccg ctgctgattt tgcgcttttt agtgcctctg
     6181 tacttccatc ataggttgct ggatttgctg aaataactgt gtcaaaagtc tccccacaac
     6241 gttcgaaacc atccagtctt ggcagaacca tgtctagcat atcggcaccg aaaacctctt
     6301 tggcctgact atggcaaatt ttgtgatcag acttgttcag gatcttggac atgaagccga
     6361 cattaccgat gagattgcag gtgtgcccat ccatttcgat ggaatctatg agggcaggga
     6421 ggctggccac aaacttcagg gaagaatgaa aatcaaccgt cgccggtgga agaggggtca
     6481 acatcaggtc tgccgcccca atgcagtttt tcaggaacgc atcaaggtga ggaccactat
     6541 caaggaaaat aaagtcgtag tcatgctgga gcttgtcgat aatgttctct ttaagcaccg
     6601 catgaatatt tttccctggc agatgttctt cacacaatcc cttccagcct tcagcaagga
     6661 atgcatcgtc aatggaagcg gggattacat caacaccagg gataatagac ggtactataa
     6721 aatcggaaag taattcttca cgagacacgt tctggagcat ggcctgagca gccgtatttt
     6781 caaccaagcc cactgaattc tcatggctca ggaacattgt cagagatgcc tgcggatcga
     6841 aatcgatggc cagaatacgc aggtcttcga acagaagctg aggatgagct cgaaaagcat
     6901 gtgataatga agcggtagaa actgtcttgg aaccaccacc tttaagatta catacaaaaa
     6961 tcgtaaaggc tttatcgaag cgatccctgt acttcggtac tttacgatgg ttatacagat
     7021 cgatgacatt ctgaatcgtc atcgcgtatt tcatcgtatt accagaaggt ttctttttaa
     7081 aaatgtagcc accggcttcc atttcggaca cagcatactc aacactgcct ttactgagct
     7141 taggcagctt atacagagcg gctttgctgt attcctgata gaactcggta agcttcaatt
     7201 cctctttttg ctgtcggata ctttcgctca aagatgtcag aagcttacct gcacgtgtag
     7261 cgactttccg taactgctca atattgtcca tttatcacct catgacgttt tttgtcattt
     7321 tgaacaaaaa gtgtcatgtt gcaagaaaaa aatcatattg ttttgatttc gctctcaaca
     7381 gaagggggat gaagctcgac gggcgtgcta ctaaatcaca ctgcagcaac acgcagacgc
     7441 acttttgacc atcaacagca actcatacaa aagggctttg agaaaggtat tcaacttgca
     7501 agacaggaga gtcattcaga aggattctga ttttagttac gaccagtgca atgctgagcc
     7561 ttatgtatca gcgctcgccg cagccatgta accgagcgta gcgagcgaac tggcgaggaa
     7621 gcgaagagga accgttccgg cagatagctc ttacgctcag cacaagaaga gttttccatc
     7681 ctctgtggga aacaggtcaa ctttaatgga cgctccggac tatgggaaac aggtcaactt
     7741 cgcggacggc ccggactatg ggaaacaggt caacttcacg ggcggccggg agtgtgggga
     7801 acaggtcaac ttcgcggacg gcccggatta tgggaaacag gtcaacttcg cgggcggccg
     7861 ggactgtggg aaacaggtca actttgcaga cggcccggac tgtgggaaac aggtcaactt
     7921 cgcgggcggc cgggagtgtg gggaacaggt caacttcgcg gacggcccgg actaagggaa
     7981 acaggtcagc ttctcggacg gcccggacta tgggaaacag gtcaacttct cggacggtcg
     8041 ggagtgtggg aaacaggtca agttaggcaa gcactatggg aaacacagca agttcagtat
     8101 ccccccatat gtcagcaaca aactaagggg ggaggaaata aaaattacag actattgttt
     8161 tttatcaaga aaattaaaca gcaagcgacc atcttcttca gtggtatatg ttacctttaa
     8221 tggcgtcttc tcattaattt tcttcagagc aggttccagg aaggtacgct tcatttcagc
     8281 aatattgttt ttctgcgagg cagggagcat aaacctttca cataaccagt catgtgtggt
     8341 gatccagaca ccagtgctcc ggaactgaca caggctttca taaagccgga tgacacgaac
     8401 actattaagc tggccacagt catagagaga ataggtagtg aactgggagg ttaagccgac
     8461 caggaatggc atgaccttgt agttaaattc aatctgccag gaaccccggc ctcgcttcat
     8521 tcctgcttca gccagccacg gacgtttaac ttcttcaaac tcaccttctt ttggataaaa
     8581 ggtaaccgtt gattcgccca gagcatttac cccagccttc acatcacgac tggcgacaga
     8641 cgtagcgaca ttgaagtatt tgacgtaatc ggaaacgctg attttgaaga gcggcaatac
     8701 gtcagagtca tcttcctggc tgtcattaaa ataggcctgc atgaggcata accacagcac
     8761 acgttttgcc tgaagaggca ggtaatatgc agcttcggtc aattcgttag attgctttat
     8821 actggcgcgt tttttaaacg ttttagtagc tggcaagagt gcttgagtat ccatttcaat
     8881 gcctatgggt aaatttttac ccacccataa tgacagtgaa aacagaaatt gcaagctatc
     8941 gcgatactta tccaccggaa aaatctgcac tttatccaca agtggataat tgagaactga
     9001 acttgacctc tttcccatag cttgttgatg cgtatcccat agcttgttga cacaaaaccc
     9061 actgatacgt gattaatttc ccatattatg tttaccagtt gcccatacag gccccctctc
     9121 aggccgcact gtacgcggct ttcaggctcc ctaaaagatg taaaataaag aaaaagaata
     9181 aaaaagtact ttataaaaat atgcccgtgg ataagtggat aaccccgagg ggaggtgatt
     9241 tttcaggcat tgagcgtaag cagaatttat aagttttgtt ccctggtgct tcctcgctca
     9301 ctcggggctt cgccctgtcg ctcgactgcg gcgagcactg ctggctcgat catatccgtg
     9361 gccggaagat actactcctc ccccgagtcg ctctccagag ccggctacag cgagacaacc
     9421 cgcacgaaga tttctatcgt ccctgcagac atattcgaat cgtttttatt aaccctggct
     9481 tccgtcgtga cagaacacta cgtcctgtaa aagggggact aaggcccctg aatcacacac
     9541 ctgaataatg cggagtttta cgataatgtc attgctggaa actttcctga ccgcttctca
     9601 gggggcaaca atcctgttcc ttgccatgct gatctactgg caaaagcgat aaatccaagc
     9661 gggctggcct ttggccagct tcgctgtata aggggaaaaa tgcggcatat ccagacattt
     9721 cactaggcaa tttgaattca gatatctatg cttgggatcc ggtcaacagg aggtaacaag
     9781 atgaaacttg aaactataca gatagataat accgtaagga gtggtcttaa ttctttcact
     9841 gacggggtga ttattcgtga tacaaaggta ttgatcacgc tcaatctccc ggatggacag
     9901 aaaaccacta ttgaggggga atggcggcat atgtcggacg ccaacaggtt gtcagaacgt
     9961 attacgatgc ttcagcatgc gatctatgag cagtatgagg aatacagagc agatattccc
    10021 gtcagctggt ccagataacg tctgacagac ctccccggcg agcaggacgt tcgccagcat
    10081 cattctccta agtacttaaa tattcccttt cagcatggct accgcatccg cttcgggcat
    10141 ctgaaactga actcgatgcc gcgcagcaac atcaagtgcg aacaccttcg tatagacttc
    10201 cgtcgagcta atcgatttgt ggcccatcag gctctgcaaa accttcagtg gtatgccggc
    10261 gtacagcatg tgcatcgcgt aggagtgacg gaacgtatgg ggggtcacag gtactgagaa
    10321 cgtcaccccg tcggctgctg cggcctccac cgcctcgcta agccaggtgc ggacggtacg
    10381 gtctgtgatc tcccagatac gcgccttctc cactttgccg gtgcgcttgt ttcgtctttc
    10441 cagcggaatt ttcagggtgg ccaccatcat ctgcagctgg ctgacgtagt ggtgatcgga
    10501 aagcggaacc agacgatgag cctgactacc ggccggcgct cgacctgctg ttctggccgc
    10561 tttctcggtg cgctgcttga gcgtggccag ctgcacgaac ggatacggtg gcgccagcga
    10621 aaaatccccc cgggtcagcg ccagcacctc gttgatacgc gccccggtgt tccagagggt
    10681 tgccagcagc attttgcggt gcaaatcggg gacgtaatgg agcagggcgc ttacttccgg
    10741 cgccagcagg tatttcggca gttcatcctg aactacagcc atctggcgca gtgcaagcgc
    10801 ggcggaataa tcgatagcaa caggtagttg cgccggtggg gtgccttgat tccaggaaat
    10861 aagttctgac atgaaggaaa atcctattct tcgtctggca atccggtgct ctctaagcta
    10921 tcaacagatt ccatcaatct aagagaagca tgatatttgt gttgactgcg ggcataacac
    10981 agctcagcat taatgctccg cgcacgcgcg attgaaagca tacgccaggc agaccagtga
    11041 tgggcagaga ggtcatcatg cggcatagaa cagatctgcc ggagctcacg cgaaagacgg
    11101 attgccctga ggccaagccg ggatgactgt ttattaattt ctgacagacc gttactacca
    11161 tcatatggat tgtcatttcc aaacgctgac tcgattgact gcttggcttc ctgctgcaga
    11221 accggcatct gactcagacg gaacataagc tccggaggaa gagatcgcca gtcaccgtct
    11281 atgccgtcat agctcaactc cggtagagta tgattcactc tgaaatgccc gtgttcgggt
    11341 tcgttaaatc cggactcgta agctgaataa acgcatcttt gcgcaaatcg ctcaagaaga
    11401 aagaccagcc gggttgcaat aaaatatcgc tccctctgca ttttatcttc tgacgccttc
    11461 ttctcccgaa tcagggtaaa ccgatgggaa atgtactggc tccctaatgc gcctcctata
    11521 cctgccagcg ctgtaatcac aggtatccac acggaaattc cgtccgccat atttgttcct
    11581 atatagagaa atcccgccac aagtgaaaag gtatgtaaca gtggtgaaca ctgctaccaa
    11641 tctccatcaa gcatttgcat ccatgcatgg cctgaatctg caagctcagt caaaactgac
    11701 gactttcctg gaggtatttc atttttagcg ttgtaattca cccgacaggc atttttgcga
    11761 aagttccagc ttttaagata aggatcatag ctggctgcta ccaggttgtc ccaggtgata
    11821 ataaggcgat tgtacttgcc attgtcacgg agccagagac gagtatgacc taagcatttc
    11881 agcgcctcat atatttcgtg tatgtcgacc tgctttcgtt tgaccattaa tactctcctc
    11941 catcctttgc atgaatatag tcactcagac taatgatgac gcacagcaca accgggtgtg
    12001 tacaaccgcg cgtctgtcac ccggatgatt ttcccctcac gtcatcattt aaatgctgat
    12061 aattagcagg catatagaca catctttaaa ttacctgacg gccgctctgg ccggatccga
    12121 cttttatgtt attccacagt ttccggaagt gtaccacgca cacggaataa ccacagcgcc
    12181 ctttcagggt ggtgagcggc cggtcacggg gcaggggagg ggcacaacat atatggtcac
    12241 tgacagctgg aaacaccgct acatcttgtg tttccgggcc gctgcgcgcc ggcggggctg
    12301 aatcagatcc ggaactgcct tttttcggat ctgagcggga aaagtgagaa accaacatag
    12361 gtgtaacata tccatatgcc gggcatctgc ataggcaaag catacgcata tacctaccat
    12421 ctgcatatgt aacacctata caagccgggc ttgcaggcat atacttcacc tatgcgggaa
    12481 tactccctgg cacactcagc atatgaggaa aaaccatgcg cacagtttcg atatttaaaa
    12541 acggcaataa ccgcgccatc cgtctgcccc gggacctgga ttttgatggc gtcagcgagc
    12601 tggagatcgt ccgggaaggg gacagcatca ttctgcgtcc cgtccggccg acctggggct
    12661 cgttcgcgca gctggacagg gccgatccgg actttatggc ggagcgtgag gatgtggtca
    12721 gcgatgaagg acgctttgag ccatgaagaa aacctggatg ctcgacacca acatctgctc
    12781 gttcatcatg cgcgagcagc cggcagcggt gctgaagcgc ctggagcagg cggtgctgcg
    12841 cggtgatcgc attgtggtct cggccgtgac gtatgccgag atgcgcttcg gcgccaccgg
    12901 cccgaaggcc tcgccgcgcc atattcagct ggtcgacgcg ttctgcgcgc gcctcgatgc
    12961 cgtcctgccc tgggaccgcg ccgcggtgga cgccacgacg gacatccggg tggcgctgcg
    13021 cctcgccggg acgccgatcg gcccgaatga cacggccatt gccgggcacg ccatcgcggc
    13081 gggcgccatc ctggtgacga acaatgtgag agagtttgcg cgggtcccgg gcctggtgct
    13141 ggaagactgg gtgaaataat cccgtaataa tttcc
//
LOCUS       DAZFYC010000135        13169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFYC010000135 DAZFYC010000000
VERSION     DAZFYC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.135"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHKW5_004291"
     CDS             <1..759
                     /locus_tag="ACHKW5_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7513545.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1129..1953
                     /gene="gadX"
                     /locus_tag="ACHKW5_004292"
     CDS             1129..1953
                     /gene="gadX"
                     /locus_tag="ACHKW5_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO7513546.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2321..3049
                     /gene="gadW"
                     /locus_tag="ACHKW5_004293"
     CDS             2321..3049
                     /gene="gadW"
                     /locus_tag="ACHKW5_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO7513547.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3412..6525)
                     /gene="mdtF"
                     /locus_tag="ACHKW5_004294"
     CDS             complement(3412..6525)
                     /gene="mdtF"
                     /locus_tag="ACHKW5_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO7513548.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLCTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6550..7659)
                     /gene="mdtE"
                     /locus_tag="ACHKW5_004295"
     CDS             complement(6550..7659)
                     /gene="mdtE"
                     /locus_tag="ACHKW5_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO7513549.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7767..8045
                     /locus_tag="ACHKW5_004296"
     CDS             7767..8045
                     /locus_tag="ACHKW5_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513550.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8046..8573)
                     /gene="gadE"
                     /locus_tag="ACHKW5_004297"
     CDS             complement(8046..8573)
                     /gene="gadE"
                     /locus_tag="ACHKW5_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO7513551.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9372..9944)
                     /gene="hdeD"
                     /locus_tag="ACHKW5_004298"
     CDS             complement(9372..9944)
                     /gene="hdeD"
                     /locus_tag="ACHKW5_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO7513552.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10199..10531
                     /gene="hdeA"
                     /locus_tag="ACHKW5_004299"
     CDS             10199..10531
                     /gene="hdeA"
                     /locus_tag="ACHKW5_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO7513553.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10647..10973
                     /gene="hdeB"
                     /locus_tag="ACHKW5_004300"
     CDS             10647..10973
                     /gene="hdeB"
                     /locus_tag="ACHKW5_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO7513554.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11037..11684
                     /gene="yhiD"
                     /locus_tag="ACHKW5_004301"
     CDS             11037..11684
                     /gene="yhiD"
                     /locus_tag="ACHKW5_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO7513555.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11726..12256)
                     /gene="dctR"
                     /locus_tag="ACHKW5_004302"
     CDS             complement(11726..12256)
                     /gene="dctR"
                     /locus_tag="ACHKW5_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7513556.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12412..12978)
                     /gene="slp"
                     /locus_tag="ACHKW5_004303"
     CDS             complement(12412..12978)
                     /gene="slp"
                     /locus_tag="ACHKW5_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO7513557.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3583 a         2979 c         3021 g         3586 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgacacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tacttttgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa cacacctgat aacataacgt tgtaaaaacc
      781 gaatgcccag cctttaaaaa aacagctggg cattcggttg cttattaatc gcaataatat
      841 attggctgtc tattcatcgt gttgataaga tatatgttat atgaatgttt attaaatgta
      901 gcgattgcct ttaatgtcac gaaaagggat tatttgcttg ctattaattt cactgcgttc
      961 ttaaacacgt ttttgtatta taaatccagt catcctgccc gtagaacatc acacattatc
     1021 atcctgttct cccgcttcgt ttaaatttat ttatcaatca atttgactta agagggcggc
     1081 gtgctacatt aattaacagt aatatgttta tgtaatatta agtcaactat gcaatcatta
     1141 catgggaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat ggttaatggt
     1201 gaatatcgct attttaatgg cggtgacctg gtttttgcgg atgcaagcca aattcgagta
     1261 gataagtgtg ttgaaaattt tgtattagta tcaagggata cgctttcatt atttctgccg
     1321 atgctcaagg aggaggcatt aaatcttcat gcacataaaa aaatttcttc attactcgtt
     1381 catcactgta gcagagatat tcctgttttt caggaagttg cgcaactatc gcagaataag
     1441 aatcttcgct atgcagaaat gctacgtaaa agagcattaa tctttgcgtt gttatctgtt
     1501 tttcttgagg atgagcactt tataccgctg cttttgaacg ttttgcaacc gaacatgcga
     1561 acacgagttt gtacggttat caataataat atcgcccatg agtggacact agcccgaatc
     1621 gccagcgagc tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga agaagagaca
     1681 tcatattcac agttgcttac tgagtgtaga atgcaacgtg ctttgcaact tattgttata
     1741 catggctttt caattaagcg agtcgcagtg tcctgtggat atcacagcgt gtcgtatttc
     1801 atttacgtct ttcgaaatta ttatgggatg acgcccacag agtatcagga gcgatcggcg
     1861 cagggattgc cgaaccgtga ctcggcggca agtattgttg cgcaaggaaa tttttacggc
     1921 actgaccgtt ctgcggaagg aataagatta tagaatttta ctcagacata aaaaaaaccc
     1981 ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac cgttataaca
     2041 ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact tttactggaa
     2101 ataagatcag ccattttttt ataaacataa gctatacgca gtgcgaaaat atattcgtgc
     2161 tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata aattttggtc
     2221 ctaccaaatc tggcagtttt ttcgctaaga aacagtctgg caacatttca ttagtatact
     2281 gaaattgaaa taatcgcagt atgaaatata agggataatc atgactcatg tctgctcggt
     2341 gatcctcatt cgtcgttcat tcgatattta tcatgaacag cataaaatat cgctgcataa
     2401 cgagagtatc gtgctgctgg agaaaaattt ggcagacgat tttgcgtttt gttcaccgga
     2461 tacgcgacga ctggatatcg atgagctgac agtttgccat tacttacaaa atattcgtca
     2521 gctaccacgc aatttagggt tacatagcaa agaccgtcta ttaattaacc agtcaccccc
     2581 catgccgctg gtgacggcga tttttgatag cttcaatgaa tccggggtaa attcaccgat
     2641 actgagcaat atgctctacc tttcctgttt atcgatgttt tctcataaga aagaactgat
     2701 ccccttactt ttcaatagca tcagcactgt ttcaggaaaa gttgaacgcc ttattagctt
     2761 tgatatcgcc aaacgttggt atctgcgcga tatcgcggaa agaatgtata ccagcgagag
     2821 tctcatcaaa aaaaagttgc aggatgaaaa tacctgtttc agtaaaatat tactcgcttc
     2881 caggatgtcg atggccagac gattactcga gttacgtcaa attcctctgc atactattgc
     2941 agaaaaatgt ggctatagca gtacgtcgta ctttataaac acatttcgac aatattatgg
     3001 tgtaacgcca catcagtttg cgcaacattc gccaggtacc ttttcctgac atattttgca
     3061 tttgaatatt ggtcaggatc tcacacctgc ttcatgtgaa actcttccct gatgatttct
     3121 gccgggctac cggctagttc tctttcgcag tttgccaggc ggtataggta ttttcaggag
     3181 gaaaagactc ggcatgtttg ggattattaa gctgacaatt catacgatta cgggaatgtg
     3241 ggtcagtatt gtgctgttca aattgatgac gaatggctgg agtggtttct actttcagtg
     3301 ctgtgtacta agcctggtat tcctgacggt tagctggttg ttgagtggtg aatggttagc
     3361 aggaaagagt aaggctgaac cttcacgttc aaccttactc tcatttacac gttacgcttt
     3421 tttaaagcgg gcaaagagat gttccactac aacgaaaaag accggaacga agtaaattgc
     3481 cagcactgtt gcggcaaaca tcccgcccat cacgccggta cctaccgcgt tttgcgcgcc
     3541 agaaccggca ccatgactga taaccagcgg cagcacgccg agaataaagg ccagagaggt
     3601 catcaggatt gggcgtaaac gcatccgcgc cgcttcgatg attgcctcta tcggcgtttt
     3661 cccttctttc tgcatcatct caacggcaaa ttcgacaatc aggatggcgt tcttggcgga
     3721 aagcccgatg gtggtcagca aaccaacctg gaagtagacg tcattactta agccgcgcag
     3781 atcggtggcc agtaatgcgc caacgacgcc taacggaaca accaacatca ccgagaacgg
     3841 aattgaccag ctctcataga gtgcggcgag ggcgaggaac accacgacca gtgaaatggc
     3901 atacagtgca ggagcctggt ttgaggataa cgcttcctga tacgacagtc cggtccatga
     3961 gtagccgacg cctgccggaa gtttagcgac caggtctgcc ataaatttca tagcgtcacc
     4021 ggtacttttc ccggccgccg cttcacctaa aatctccatt gacgggatgc cgttgtagcg
     4081 ttccaatcgc ggtgaaccat aggtccattc ggtagacgag taggcagaaa gcggtgccat
     4141 cgtgccagag gcgttgcgta cataccattg gttgatgtta tccggcaaca tacggaacgg
     4201 cgtgcctgcc tggacataca cttttttcac ccgcccctgg ttgaggaagt cgttcacgta
     4261 gctgctgccg aaggcggtgg aaattgtctg gttgatatca gacagcgcca cacccattgc
     4321 ctcagctttc gcggcgttga cgttcacttt gaacatcggc gtatcttcca ggccgttcgg
     4381 gcgtaccccg gtgacctggt caggcgattg cgctgccagt gataacagct cgtttcgtgc
     4441 ctgggttagt ttttcgtgcc ccaggttacc gttgtccagc agttccatat caaaacctga
     4501 cgcggtaccc agttcagcca ccgcgggtaa gttgaacggg aagacgacgg ctttattgat
     4561 actgcttaac gcaatcattg cccgctgaat gatcgcggta accgagtttt cctcaccgac
     4621 acgttcagac cacggcttga gactgataaa cgccaggccg ttgttttgcc cctggccgct
     4681 gaagccaaag ccgccaacgg taaacaccga ctgaacatta tctttctctt ttgtaagata
     4741 ataatccgtc acctgttgca gcactttcgt ggtgttaacc atggtggcac cggaaggtaa
     4801 ctgcgcggtg gtcataaata ccccctgatc ctcttctggt aagaaagagg tcggtgtaca
     4861 caggaacagc accgccatcc cggcgcaaat cagcaggtag accaccatgt agcgaccggt
     4921 acaacgcaac agcgagcggg tgctatcggt atagtgctga gttgattttt caaacagcgt
     4981 gttgaagcgt gcgaacaggg cgttaggttt gtgaccgcct tccggcgcgg ctttcagaat
     5041 ggtggcgcac agggcagggg tcaggctcat tgccacaaat actgaaagca gcatggagga
     5101 gatcagcgtg atggagaact ggcggtagat ctccccggtt gcgccgctca taaaggccat
     5161 cggcataaac actgcggaaa gaacaacagc aataccgacc agcgcacgtt ggatctgccc
     5221 catcgattta tgcgtcgctt ccttcggtgg tagcttatct tccgcaatga cacgctcgac
     5281 gttctccacc accacgatgg cgtcatccac cagtagccct atcgccagca ccatcccgaa
     5341 catagttaac gtgttgatgg tgaaaccgac cgccgacaag atcgcaaacg tcccgagaat
     5401 aaccaccggt acggcaatcg tcgggatgat tgtggcacgg aaattctgca aaaacagata
     5461 catgaccagg aagacgagga tgatagcctc aaccagtgtt ttaaaaactt cctgaataga
     5521 aatttcgata aacggcgtgg tgtcgtaagg ataaaccgtc ttcagacttg cggggaaata
     5581 ggctgataag cggttcagct cctctttgac tgcccgcgag gtatccaggg cgtttgctcc
     5641 ggtagccagt ttgatggcga tcccggcagc aggtttgccg ttatagcgcg ccacggtgga
     5701 ataatcttcc gctccaagtt cgacgcgggc gacatcacgc agcagcactt gcgaaccatc
     5761 ttgctgaact ttcaacagga ttttgccaaa ttcttccggc gtttgcagac gcgtctgcac
     5821 aatgatcgag gcgtttagtt gctggtctgc cgcctgtggc atgccaccca gttgaccacc
     5881 ggaaatctgg ttgttttgca ccttaatctg ggaaataaca tcggaaggta ccagattgta
     5941 tttattgagt ttttgcgggt ccagccagat acgcatggcg tactcggaac caaagagctg
     6001 tacgctacca acgcccgcgg tacggcttag cgggtctttg atattagacg ctacatagtc
     6061 cgcgatatcg tactggttga ggctgccgtt atcagaaata aacgccgcta ccatcagaat
     6121 attactgctc gacttatcaa cgctaatccc ctgctgctgc accgcttcag gtaatgaagg
     6181 catagcgagt tgcagtttat tttgcacctg aacctgtgcg atatcaggag atgtcccagt
     6241 ctcgaaggtc agagtgatag aggcattacc cgccgcatca ctggttgaag acatgtacat
     6301 caggccatca agcccattca tattttgctc aatcacctga gtgaccgagt cttctaccgt
     6361 ttgcgcatcg gcacctggat aggtagcgct gacggtaatg gtcggtggcg caatctgcgg
     6421 atactgcgca accggtaagt tcatgatcgc cagaccacct gcaagcatca taataatggc
     6481 aagtacccag gcaaaaaccg ggcgatcaat aaaatagtta gccatgaaag tccccttaag
     6541 cctgcaacgt tattgtttcg attcggtgct ggcgttttcc tggctggagg aaattgctcg
     6601 tgctttgata cccggacgaa tgcgttgcaa accggaaacg atcacccgat cgccagcctg
     6661 caagccagag gtgacaaccc actggtcgcc gatggctttg ctggcttcaa tttcgcgtag
     6721 ctgcacaaca tcgtctttat ccagaatgag cgccgttgct ttaccctggg cgttgtgggt
     6781 gacgccttcc tgcggcacca gtaatacatt ctggcggcta ccttcatcca ctaatgccgt
     6841 gacgtacatg ccaggcagca agtcaccatt tgggttgggg aaaatcgccc gtaacgtcac
     6901 ggagcccgtg gtttcatcca ccgtcgggtc ggagaatttc agcgtgccgg tctggctgta
     6961 gcgtttacca ttttccagat tgagctgtac tggcgtactg ccctgaacct gtttgatttg
     7021 cccactggcg acctcttctt tcatgcgtaa gaaatcctgc accgactgcg tgagatcgac
     7081 ataaatcggg tccagacgtt gtacggtaac cagcgaatct gcctgattag cggtaacgag
     7141 tgcgccgacg gtcaccgacg atttcccgct gacgcccgta atcggcgagg tgacattcgc
     7201 gtattgcaga ttgatcgtcg cctgttcaac agccgctttg gcgacggtga cattggcttc
     7261 tgcttcattc aactgggtgc gcgcggtgtc gtaatcctga cggctaacgt agttggtctt
     7321 cagcaacgat gcctggcggt taaaggtgat gcgggcattg ctggcggtag agagcgcttt
     7381 cgccagcgag cctttggcgg agtttagctc ggcctgtaaa ggtgcaggat caatctgata
     7441 cagcgaatcg ccctggttca ctttatcgcc ttcgataaag ttgcgtttaa tgataatacc
     7501 gcccacctgg ggacgtatct cggcaacttc ataaggaacg gttctaccgg gcaattcgct
     7561 caacacattg accgaaccgg gggagagtgt gacgacaccg acctcaggcg tcatggcggc
     7621 ggcgttttcc gccgatttgt catcgcaggc ggtgagcatc gcgccgcaga aaaacaacgg
     7681 tattaacagc tttcttcttc tgttcatttt agtccctgaa aattcttgag aaataacggt
     7741 tctttaaatt gcgcgcgagg agagatatga gggatttaca gaccagcggc atagtggggt
     7801 tgtcagccag taaggtggga tacaggcaca gtgatcgaca tggtgaggtc aacgacaaaa
     7861 aaaatgttgc cgttctgcca acagttcctg ccagcattcg ggcaacgaag ggttctactg
     7921 gtggatacac ataccagggg aataaagtcg ttttagcctc tcctttagta atcaccgggg
     7981 gtaatgaaat cagcatctgc atccctcgtc atgccagcca tcaatttcag ttgcttatgt
     8041 cctgactaaa aataagatgt gatacccagg gtgacgatgt cgctcatacg tcttagtttc
     8101 agcttgtaca taatgttgaa tttctgaatc tttaatgttt gttgcgaaat attgagcgtc
     8161 gacgtgatat tgctttcatt tttatttttc agaatcagtt gcataatttt gaactgatta
     8221 tgtgaaaaac ataattgtga attcttatgg ggcaagtgtt taccataagt aatctcaaga
     8281 agatttttga tactttcttt gcggctaaca aaataaatag gcgctttagc ttttagttta
     8341 ctgatgtgat aaggtgccaa aacaataatt ctctcggcat ctaatttctc cagaaattta
     8401 atcgcttctt catcaaggat atgattgtga taggtatcaa tgataacctt gaagggttta
     8461 ttgcctactt ttttgatctc tgacagggaa ttgattttta tcatttcgtg attatctttc
     8521 aactgccaaa agccctgtaa aagaaaagaa tctttcgtca tgagaaaaat cataacttgc
     8581 tccttagccg ttatcgtttc gaacatgtca tccttttcat tatttacatc cttgtccgaa
     8641 tcgttgttca atatagtaaa cgcctataag aactttgccc ttcgtgaact aatacgtcta
     8701 ttaacactaa tcaatacttc agatttatcc atcacgcact ccatatattg atcctaagta
     8761 agattcctgg ttgttatcaa tttgtaaata tcaaacaaaa agttaacgtt tgcggattac
     8821 tgcccaagaa taagttatca ataaccagtt tgtgatctct gaagaatatt actaaagtta
     8881 aaatgtaatc cgatttaaat atcgagtctc cttgtttcga cttaagctgg caattggatt
     8941 gccagctttc ttttaacgca ttgaaattac ctgagtaaat aacgcattca tgtcttcagg
     9001 tttttatata gcgtctggag acacggatat ttatgcaatg taatcaacgc cagttgcaaa
     9061 aaaatgaacc cggattactt atttaaccgc ccatagcatc aacgcttacg gaactctttg
     9121 ttcactttcc cacagcactt ttaaaacatt aaacctgact acggaaaata tcagccatga
     9181 taatgtttga aatggctaat ttgccatgga gtgaaaaaaa ttagatgaaa ttcagtaggt
     9241 tgaaatgatc actagcaggt aattatttca atgatagtgc gcaattgatc tacaacactg
     9301 cgtagcggag agagtattaa tcggatcata gtcacatcaa gtgactatga tccgggtgac
     9361 aaccgggata attattgctg cttaacgaac aaactggcga agctgaacag gctggcggcg
     9421 ctaaatatca gttcaattcc caccagtgtg gaaaccagcg ttacagacac catcggcgtt
     9481 gcaccaagga atatccaggc aatgacgata tccagcacac caataacgag ctgtagccag
     9541 ctgcctttca ttgaacgctg acgataccaa ctcatcaggc gaataacccc tgcaacacag
     9601 aacaaaccgg caataaatgc cgcaatggca aaaatgccca gctccggtgc gcggatgaag
     9661 aaatagccga tcaataaata ggcgactgcg acgaggaaac cggataatac cggccagaaa
     9721 ttatgactgc ggttgctgaa taacccgaca ataagcgcaa tacccgagca gattaataat
     9781 gcacccacta ctgtgcttaa aatatcgcca gagacgaacg ggaaactgat acacagcaac
     9841 ccgacgataa acagcagcac ggcaataaac tggattgctc tgcgatgctt tttcagcatc
     9901 tccagatcaa acttcaaaat tgttgcctta tctatatata acatagaacc accctataaa
     9961 attaagaaga aaatcccctg ctatcaatct atgccaaaaa cgcgtctaag aatgcagtcg
    10021 atttaataaa aatttcctaa ttgcagtatc tgatgcatct gtaactcatt gtattgaaat
    10081 aaaaatatct gattttgata ttttccatca acatgacata tacagaaaac caggttataa
    10141 cctcagtgtc gaaattgatt cgtgacggct ctttcacttt atagttgagg atattacgat
    10201 gaaaaaagta ttaggcgtta ttcttggtgg tctgcttctt ctgccagttg tgagcaatgc
    10261 agcggatgcg caaaaagcag ctgataacaa aaaaccggtc aactcctgga cctgtgaaga
    10321 tttcctggct gtggacgaat ccttccagcc aactgcagtt ggttttgctg aagcgctgaa
    10381 caacaaagat aaaccagaag atgcggtttt agatgttcag ggtattgcaa ccgtaacccc
    10441 agctatcgtt caggcttgta ctcaggataa acaagccaac tttaaagata aagttaaagg
    10501 cgaatgggac aaaattaaga aagatatgta attccgggaa tgcgttacat cgtacttcct
    10561 tgcatattga acaggccgga atatcttctt taaaagcagc tattcctcct gttcatatat
    10621 aatctctata ttgaatgggt tacaaaatga atatttcatc tctccgtaaa gcgtttattt
    10681 ttatgggcgc tgtagcggct ttgtcactgg tgaacgcaca atctgcgctg gcagccaatg
    10741 aatccgctaa agatatgacc tgccaggaat ttattgatct gaatccaaaa gcaatgaccc
    10801 cggttgcatg gtggatgctg catgaagaaa cagtatataa aggtggcgat accgttactt
    10861 taaatgaaac cgatctcact caaattccta aagtgatcga atactgtaag aaaaacccgc
    10921 agaaaaattt gtataccttc aaaaatcaag catctaatga cttgccgaat taatgaggta
    10981 caagtaaaaa ggagtagcaa gttgagccat cttgctgctc ctttttgcat ttttatatga
    11041 cagcagaatt tattatacgt cttatactcg cggcaattgc ctgtggcgct attggcatgg
    11101 aaaggcaaat gcgcggcaaa ggagcagggt tacgcacaca tgtattaatt ggcatgggaa
    11161 gcgccctgtt tatgattgtt tcgaaatatg gttttgctga cgtgctgtct ttagatcacg
    11221 tcggactcga ccccagccgt attgctgctc aggtggtgac gggcgtcggg tttatcggtg
    11281 caggtaacat tctcgttcgt aaccaaaata ttgtaggtct gacgacggca gcggatatct
    11341 gggtgaccgc cgccataggt atggttattg gcagcggtat gtacgaactg ggtatttatg
    11401 gttcagtgat gaccttgttg gtgctggaag tcttccatca attaaccttc cgcctgatga
    11461 acaaaaatta ccatttacag ctgacattag tgaacggtaa tacggtatca atgctcgact
    11521 ggtttaagca gcaaaaaata aaaacggatt tagtttcgtt gcaggaaaat gaagatcatg
    11581 aagtggtcgc cattgatatc cagcttcatg ccacaacgtc gatagaagac ctgttgcgat
    11641 tattaaaagg tatggcaggg gtgaaaggcg tttcaattag ctaatctgtg tggcagcgta
    11701 gccagactca ccgtaagcct gaaattcaca ccagataatc aatatgctga tggcgtacca
    11761 gctcattgat ccgcttaaga ttgaggatca tcatgatatt gtgtttatgg cagtagaacg
    11821 tttttagcga tattttcagt ttctccagaa tatcttccgt tgtcatttga ttcgcaatgc
    11881 agctcagaac ctgatgttgc ttgcgcgaaa gtgacacagc cgcttcaggc acagagcagt
    11941 aactgccatt gatgagtgcg acaaaatcaa tgatagtgat agtccgcgga acaaaggtca
    12001 ccggcacttt ccccaggcag cgtttaatat taaaggggga gaaaataatc acatggacag
    12061 gttttaatcg ttcaatttga gtgagaaaat tagaatgaaa tacattattc attaatgtat
    12121 caataatgac gaaggcattc tgatgcaaca ttacgtcgat ctcttcttcg ttctgtatat
    12181 gaacgacatt acctttactc agaatgtttt tcatcgcggt gaagaacatc gtatccctgg
    12241 taattataag aaacataacc gactcctggt gcggacatta actttataat cagtttgcca
    12301 aaggtaatta acaggacagg gcttataata ataatagtta gcttactgta tattaatttt
    12361 atgtaataca tgatgcagat gctgagaaag catctgcatc tttcggtggt gttatttgac
    12421 cagctcaggt gttacctgac tcaccgcatt ggtgtagtaa ggcgcacccc agcccggttc
    12481 cggccagaat gcaccatagc cgtaatccca caggttatag gtggtattaa ccacttctct
    12541 caaatgccac acctggatgc cctgcatatt cacttccagg aagttatacg ggactttatt
    12601 gataaagcct ggttgttcac cctgaatggt accgaggatg gtaacaaagt gattacgata
    12661 gttcactgga tcaaggaagc cgctttgtct ggcgagcagt cggccctgat agttggcttc
    12721 aatatcaggc ttcgcatagc tatccaacgg taatacagcg atttctaaca acgtatccgt
    12781 tttgccattg ataacgttga taaccttccc accaaagcgc gcttgttgac caacatataa
    12841 ccccggctgg ttatgaacag caacaaaact tttttgaata tcaggttggt tattgccttt
    12901 gatattttgc ggaattgaac tacatgcggc aagcaaaaat gaaaggctga ggatgagtgc
    12961 accttttgtc atgttcatgt tactatcctt atcaacaaat attaaaacca taatagatat
    13021 ttctttatag ttttcatctg attctgaggt gatatgcctg atagtaacgt aaacctgtct
    13081 gatgtttata tcccttctgt ttagttcaat tccgttattt tggggcgtgg tatgagtatc
    13141 catcgcccaa taatataacc ctttgtttt
//
LOCUS       DAZFYC010000136        13066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFYC010000136 DAZFYC010000000
VERSION     DAZFYC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.136"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(71..217)
                     /gene="ygdT"
                     /locus_tag="ACHKW5_004304"
     CDS             complement(71..217)
                     /gene="ygdT"
                     /locus_tag="ACHKW5_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO7513558.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            403..1092
                     /gene="mutH"
                     /locus_tag="ACHKW5_004305"
     CDS             403..1092
                     /gene="mutH"
                     /locus_tag="ACHKW5_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO7513559.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDIIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1161..1874
                     /locus_tag="ACHKW5_004306"
     CDS             1161..1874
                     /locus_tag="ACHKW5_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7513560.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2012..2230
                     /gene="ygdR"
                     /locus_tag="ACHKW5_004307"
     CDS             2012..2230
                     /gene="ygdR"
                     /locus_tag="ACHKW5_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO7513561.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2338..3378
                     /locus_tag="ACHKW5_004308"
     CDS             2338..3378
                     /locus_tag="ACHKW5_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO7513562.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="ACHKW5_004309"
     CDS             complement(3410..4603)
                     /gene="lplT"
                     /locus_tag="ACHKW5_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO7513563.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4596..6755)
                     /gene="aas"
                     /locus_tag="ACHKW5_004310"
     CDS             complement(4596..6755)
                     /gene="aas"
                     /locus_tag="ACHKW5_004310"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO7513564.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7341..8372
                     /gene="galR"
                     /locus_tag="ACHKW5_004311"
     CDS             7341..8372
                     /gene="galR"
                     /locus_tag="ACHKW5_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO7513565.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="ACHKW5_004312"
     CDS             complement(8379..9641)
                     /gene="lysA"
                     /locus_tag="ACHKW5_004312"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO7513566.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGLVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9763..10698
                     /gene="lysR"
                     /locus_tag="ACHKW5_004313"
     CDS             9763..10698
                     /gene="lysR"
                     /locus_tag="ACHKW5_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO7513567.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="ACHKW5_004314"
     CDS             complement(10685..11377)
                     /gene="ygeA"
                     /locus_tag="ACHKW5_004314"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO7513568.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIDECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11506..12924)
                     /gene="araE"
                     /locus_tag="ACHKW5_004315"
     CDS             complement(11506..12924)
                     /gene="araE"
                     /locus_tag="ACHKW5_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO7513569.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         3322 a         3504 c         3210 g         3030 t
ORIGIN      
        1 gtaatttgag taatgaaaaa gagtaattcg tgacccaggt cacacctctc atttacgggt
       61 tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg tgccactaag ttaagcactg
      121 aaccactaaa aactggagtt tcatcgcacg tcaaggctgt aaatggaaac agtagtggag
      181 gtttttcaca gttatcccag ctttctgtgg ataacatggt gtaagatcct gtttattttc
      241 agtgaccaga tttggaaaac ctattgcagt gttgcgcaac tcgattaccg gcagcctaaa
      301 aagcaatata aatcagggaa ttgaacaaca catgtggaaa aagttacact gcgaatattc
      361 ggcacataat tgctgtttgt tttttaatca aggtatcatg acatgtccca acctcgccca
      421 ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc aagcacagca actttctggt
      481 tatacattgg gagaactggc ggcacttgcc gggcttgtta cgccagagaa tttaaaacgc
      541 gataagggct ggattggcgt gttactggag atctggctag gtgccagcgc agggagtaaa
      601 cctgagcaag attttgccgc tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt
      661 ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt taacgggtaa tagcggggtg
      721 acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg tactgtggat accggttgaa
      781 ggcgagcgca gcatcccgct ggcgcagcgt cgcgtaggat caccgttgct gtggagcccg
      841 aatgaagagg aagaccgaca gctacgcgaa gactgggaag aattaatgga tatcattgtt
      901 ctcggtcagg ttgagcggat caccgctcgt cacggggaat atttacagat aagaccgaaa
      961 gcagcgaatg cgaaagcgct taccgaagcc attggtgccc ggggcgaacg gattctgacg
     1021 ctgccacgcg gcttttattt gaagaagaat ttcaccagtg cactactggc ccgtcatttt
     1081 ctgatccagt agccatcgct ttgacctgcc gctttccggg catataatta ccgcttcatt
     1141 tttttggcag ggctttttag atgttatttg catggataac cgatcctaac gcctggcttg
     1201 cgctcggtac gctgacgctg ctggagatcg ttcttgggat cgacaatatt attttccttt
     1261 ctctggtggt ggcaaagctt cccacagcac aacgtgctca tgcgcgccgt ctggggttgg
     1321 cgggagccat ggttatgcgt ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga
     1381 caaatccgct ttttacaata ttcagtcagg aaatttctgc ccgtgatttg attctgcttc
     1441 tgggtggctt gttccttatc tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg
     1501 aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg cgctatcgtg cagattatgc
     1561 tgctggatat tatctttagc ctcgactcgg tgattacggc tgtgggtctg tcagatcacc
     1621 tgtttattat gatggcagcc gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct
     1681 cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat gctggcgctc tctttcctga
     1741 ttctggtggg ctttaccctg attctggaaa gtttcgacat ccacgtaccg aaaggttaca
     1801 tctacttcgc gatgttcttc tctattgcgg ttgaaagcct caacttgatt cgcaacaaaa
     1861 agaatccgct ctgatactcc gtacgctctc ctgcgggagg gcgtactcac tccttttacc
     1921 ctcacagttt aagattttcc tgctttcaag atatataacg tcggtttata aacagactat
     1981 tatcataggt aggccaaatg aggataaccg gatgaaaaaa tgggcagtaa taatttctgc
     2041 agtcggactg gcgtttgctg tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga
     2101 tggccgtatg attttgaccg atggaaaacc tgaaattgat gatgataccg ggctggtgag
     2161 ttatcacgat cagcaaggta acgcgatgca aattaaccgt gatgatgttt cgcaaattat
     2221 tgaacgttaa caaataaggt cagcatccgg ctggccttaa gatttttctc tttccctttt
     2281 cccttccctc tgccattttt atattcctta tgtcgtgatt ataaaaagga aacggctatg
     2341 caatatcacc gtatacccca cagttcgctg gaagtcagca cgctggggct tggcacgatg
     2401 acgtttggtg aacagaacag cgaagccgac gcccacgcac aactcgacta tgccgtcgct
     2461 cagggcatta accttatcga cgttgccgaa atgtacccag tacctccgcg ccccgaaacg
     2521 caagggttaa ccgaaaccta cgtcggcaac tggctggcga aacatggcag ccgcgaaaag
     2581 ttaattatcg cctccaaagt gagcggaccg tcgcgcaata atgacaaggg catccgcccg
     2641 gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc atgacagcct caagcgccta
     2701 cagactgatt acctcgatct ttatcaggtg cactggccgc agcgcccgac caactgcttc
     2761 ggcaaactcg gttatagctg gacggattct gcgcctgcgg tttcgctgct ggatacgctg
     2821 gacgcactgg cagagtacca acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa
     2881 actgcatttg gcgtaatgcg ctacctgcat ctggcggaca aacacgatct gccgcgtatt
     2941 gtcaccattc agaaccctta cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa
     3001 gtcagccagt atgaaggggt cgaactgctg gcctattcgt gcctgggttt cggcacgctg
     3061 accgggaaat atctcaatgg tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg
     3121 ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc
     3181 agacgtcatg gcctggaccc tgctcagatg gcgctcgcgt ttgtacgccg tcaaccgttt
     3241 gttgccagca ctctgctggg cgcaaccacg atggatcagc tgaaaactaa catcgaaagt
     3301 ttgcatctgg agttaagcga agacgtatta gctgaaattg aagcggtgca tcaggtttat
     3361 acttatccgg caccataaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc
     3421 gctgccagat ccacagcgcc gttattgcca gcgcaaacag cgcaccgaag ccaatgccaa
     3481 tgggcacgac cgggatgcct atcattaccg ccagcgagta aatgcccagc atcaacaaca
     3541 tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt
     3601 taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc atcacgccaa
     3661 tcagcatcaa caaggcatag gctggcagca gctcgtgttg cagggaaaaa atcagtacca
     3721 ccacgccaat caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaactaact
     3781 tcgctgccgc acctgcgcca accacgatac caatcgctac catcgcgttg agataggtgg
     3841 gcgtagcgtt atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca
     3901 gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat
     3961 tgcgccatag cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg
     4021 actgccccgg acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac
     4081 cgtaggccag tgcgcatgcg gccagggcga cgaggacatg ccagtcagcc agcacaccac
     4141 cggctacgga accgagcaaa atcgccgcta tggtagaagc ttccattaaa ccgttagctt
     4201 tcactaactt actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg
     4261 ctgcagcacc aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac
     4321 tggctgcgcc cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc
     4381 tatccgccac ctgcccgaca aacggcgcaa aaagaatgta agcacctaca aacaccattt
     4441 gcaggatggg ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa
     4501 acagtagggc attatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc
     4561 ccttcgacca caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccgcttcg
     4621 tctacccagc ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt
     4681 ttcagatagc gaatatcgcg cggtacagca agctccggca cgccgtgctc gcgggcatac
     4741 tgttgcaact tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct
     4801 ttgctggcat cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc
     4861 agttgttcca ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg
     4921 cggccctgaa tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac
     4981 cagccgcgct ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc
     5041 ttctccaccc gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc
     5101 tcttcgatac cagggaccga caacaggcgc gcatccattc ctggtagaat acgccctacc
     5161 gtaccgggtt tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcacg
     5221 ccgtagcctt caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct
     5281 tgtaattttt ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg
     5341 aaacgcgcgt agtgaccgag gaaagtcgag gtgccgaaca acacggtgca actgcggtca
     5401 tacaccaact ccggcacaat gcggtaatgc agcgggcttg gataaaggaa cacttctgca
     5461 cctgtaagca gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaac
     5521 gccgacataa agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacattc
     5581 gccagaatgc ttttatggct atggacgacg cctttcggat ggccttcaga accggaggta
     5641 aagaggatca gcgcctcttc ttccggctgc tgtttaacct gtgccagacg cggcatcagc
     5701 aaatgagcga agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga
     5761 tagacccagc gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga
     5821 aactggcggg aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagccct
     5881 tttaccccgg cagtgtagtt catcattgcg ggcatgcggc gacgggcgat ggccccaaaa
     5941 atcactgccg cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca
     6001 ctgtattttt caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag
     6061 tctggggtaa agttgacgtc ttcgacacat ttcttcccgg ctccgaagcg gtacattgca
     6121 ctcagtaaag attcgtacag cgtttcacgc gggcgcaccg ccattcgcgc ttccatcatt
     6181 atttgatgca gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc
     6241 atcgccacct gcgttggtgg caaaatatgc agagtaattt gcgggaacaa gcgacgttta
     6301 accagacctt tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata
     6361 accgttgcac cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag
     6421 cctgtagtgg tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga
     6481 cgtaccagat gtttaatagc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat
     6541 gatttcagcc aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga
     6601 cgcacaggta aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta
     6661 attagaacgc gctcgccctt cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa
     6721 acacggcaca aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag
     6781 taaaagcgaa tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg
     6841 cgaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac
     6901 cgaataatct caccaatcaa tacctctggg atcttgattg tggtctgcac gacgactctt
     6961 cgccagcgag aaaacgcaaa ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt
     7021 tactgttaca gattgatgac cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa
     7081 ttcatgtgct caacccgaag ttgacttcac ctatcaatac ctctgggatc accactttag
     7141 caacctgaag ccaaacgcca ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca
     7201 aaggtttgca atctcttttt ttgtcttact tatttgcgtt tggctcacat tcccacgatg
     7261 aaaacacgcc accccttgaa ccaacgggcg ttttccgtaa cactgaaaga atgtaagcgt
     7321 ttacccacta aggtattttc atggcgacca taaaggatgt agcccgactg gcaggcgttt
     7381 cagtcgccac cgtttcccgc gtcattaata attcacctaa agccagtgaa gcttcccggc
     7441 tggctgtgca tagtgcaatg gagtctctta gctatcaccc gaacgccaac gcccgtgcgc
     7501 tggcgcagca gaccactgaa acggtcggtc tgatcgttgg tgatgtttcc gatccgtttt
     7561 tcggtgcaat ggtgaaagcg gtcgaacagg tggcttatca caccggtaat ttcttattga
     7621 ttggcaacgg ttaccacaac gaacaaaaag agcgtcaggc tattgagcaa ctgatccgcc
     7681 atcgctgtgc tgcgttggtc gtccatgcca aaatgatccc ggatgccgat ttagcctcat
     7741 taatgaaaca aatgcccggt atggtgctga tcaaccgtat cctgcctggc tttgaaaacc
     7801 gttgtattgc tcttgacgat cgttacggtg cctggctggc aacgcgtcat ttaattcagc
     7861 aaggtcatac ccgcattggt tatctgtgct ctaaccactc tatttctgac gccgaagatc
     7921 gtctgcaagg gtattacgat gcccttgctg aaagtggtat tgcggccaat gaccggctgg
     7981 tgacatttgg cgaaccagac gaaagcggcg gcgaacaggc aatgaccgag cttttgggac
     8041 gaggaagaaa tttcactgcg gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg
     8101 gcgttctcaa tgataatggt attgatgtac cgggtgagat ttcgttaatt ggctttgatg
     8161 atgtgctggt gtcacgctat gtgcgtccgc gcctgaccac cgtgcgttac ccaatcgtga
     8221 cgatggcgac ccaggctgcc gaactggctt tggcgctggc ggataatcgc cctctcccgg
     8281 aaatcactaa tgtctttagt ccgacgctgg tacgtcgtca ttcagtgtca actccgtcgc
     8341 tggaggcaag tcatcatgca accagcgact aaccgcagtt aaagcaattc cagcgccagt
     8401 aattcttcga tggtctggcg acggcgaatc aaccgcgcct gaccattatc aaacagaact
     8461 tctggtaaca gcggacggct attgtagttg gatgacattg atgcgccata tgcccctgta
     8521 tcatgcagta ccagataatc acccgccttc acttccggca aggcgcgggt ttcaacattt
     8581 cccccttcct gctgggtaaa gacatcgccc gattcacata atggcccggc gacgacggtt
     8641 tccaccgttg gcgcgtgttc cagagaacga ccatcagctg ccagggcact gatatggtgg
     8701 taactaccgt acattgccgg gcgcatcaga tcgttgaacc cagcatcaac cagcacaaag
     8761 tggcggctac ccatttgttt gacgctccgc acctgagtaa ttaatacgcc agactgcgct
     8821 accaggaagc gacccggttc aatttccagt ttcacagggt ggcccaaatg gcgggcgatt
     8881 tgctcacgcg cggcattcca cagaccataa taatgttcgg tatcaaccgc ctcttcaccc
     8941 tgttgataag gaacagaaag cccaccgccc gcagagatag cctgtaaatc ctgaccgaat
     9001 tcgatgacct gacgcaccat agcaccacac acctgttcca gatgggcata atcaacgcca
     9061 gaaccaatgt gcatgtgaat gccgaccagc tgcagatgat gacgttgtat cacgtccagt
     9121 gcggcgggca gatcggtgta ccagataccg tgcttgctgt tttcgccacc ggtattggtt
     9181 ttttggctat gtccgtgacc aaaccccgga ttaacgcgca gccatacccg atgccctggc
     9241 gaaaccaggc ccagttggtc gagcatatca acagaacccg cattcaccgg aatttgcaat
     9301 tcactgacgc gttcaagcgt cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg
     9361 gggtgcgttt gcggattgta acccgccgcc aacgcacgct ctatttcgcc taacgagacg
     9421 gaatccactt tcacgccctg ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct
     9481 ttctgtgcaa agcgcaccac atcaaactgt ttcagcgctg caatctgccg acgaataatt
     9541 tgcgcatcgt agacccacac cgggcagcca aattcagcgg gcaaacgcag cagattttcg
     9601 gcggtgagat cggtatcggt gctgaacagt gaatgtggca taacaaactc cagataagtg
     9661 cttttttatg attacgccac atcataaaaa gaataaaaaa tatcgattta tgtcgagtct
     9721 atgcaaaaat gatatggatt accggattgc gagagagcgc taatggccgc cgttaactta
     9781 cgtcatattg aaatttttca tgcggtaatg accgccggaa gcctgactga ggcagcacac
     9841 ctgctacaca cctcacagcc aaccgtcagc cgcgaactgg cgcgctttga gaaggtgatc
     9901 gggctaaaat tgtttgagcg cgtacgtgga cgattacatc ctaccgtgca aggactgcgt
     9961 ctgtttgaag aagtgcaacg atcctggtac ggactggatc gcattgtcag cgccgcagaa
    10021 agtctgcgcg agtttcgcca gggagaactg tctattgcct gcctgccggt cttttcgcaa
    10081 tcttttttac cgcagctcct gcaacccttt ctggcacgtt atcccgatgt cagcttaaat
    10141 atcgtgcccc aggaatcacc gctacttgaa gagtggctct cggcccagcg tcatgattta
    10201 ggactcactg agacactcca tacgcctgcg ggaacagaac gtaccgaatt actctcttta
    10261 gatgaagtgt gtgtgttacc tccgggtcat ccgctggcgg taaaaaaggt attaacgccg
    10321 gatgattttc agggtgagaa ctacatcagc ctttcccgta ctgacagcta tcgccagttg
    10381 ctggatcagc tatttactga acatcaggtt aaacgacgca tgatcgtaga aacccacagc
    10441 gccgcgtcag tctgcgcaat ggtacgggcg ggggtaggtg tttcggtggt taacccgctc
    10501 accgcactgg attatgcggc aagcggttta gtggtgcggc ggttcagtat tgcggttccg
    10561 ttcaccgtca gcctgatccg ccccctgcac cgcccgtcat cagcgctggt gcaggcgttt
    10621 agtgagcatt tacaagcggg gttaccgaaa ctggtcactt ctcttgacgc tattttgtcg
    10681 tcagctacga cagcataaaa gcgacagcat cctcggcatg gatcgccgca gtatcaaaca
    10741 caggcaaaac actgcgctct tctggcacca gtaaaccaat ttctgtgcag ccaaaaatga
    10801 cgccctgtgc gccctgttct gcaaggcgag caatcacttg cgcataataa gcgcgtgacg
    10861 cttcggtaaa ttgccccaga cacagttctt caaaaataat ctgattaatt ttcgcccgtt
    10921 catccgcttc aggaataaga caattgatgg aaaattgttc cgtcagccgc ccgcgataaa
    10981 aatcctgttc catggtgtaa cgcgtaccca gcagcgccac acgagtcatt ccggccccgg
    11041 taattgcacg tccggtggca tccgcaatgt gtaagaaagg cagagtgcaa cgtgactcaa
    11101 tggcatccgc cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc
    11161 gctgtaagcc aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac
    11221 gctggcactc atctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa
    11281 gcccaccaag ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt
    11341 cccagctcat tcctcccagc aaaccaattg ttttcattcc tctctcctga tgtgtgttac
    11401 cgcaacaagg ctgaataagg aacgagacaa acgcctcaac ggccaggtgc ccgatctcta
    11461 ttaacgaaaa aagggccgga tgtacagcac atccggcccg tgaaatcaga cgccgatatt
    11521 tctcaacttc tcgcctgcca tcagtttgcg ttcgatatgt tccagcgtga catttttggt
    11581 ttccggaatg agccagaaag tgatgcccac aaacgcaatg ttcagcgcag tgtagagcca
    11641 gaacgtaccg gcagcgccaa tgctatcaag cagtgtcagg aaggtcgcgc cgataatcat
    11701 attcgacacc cagtttgtcg tcgtcgaaca ggtaataccg aaatcgcggc atttcagcgg
    11761 ctgaatttca gagcacagga tccacaccac tggcgcggcg ctcatcgcat aaccggcaat
    11821 acacatcatc gtcatgccaa cagagagcca ggacaagcca ctggaagccg taccgttatc
    11881 aaactgcatc aggcaatagc ccagcaccag agtgcctaac gccatcacgc tgaaaccaat
    11941 tttcagagcc ggtttacgac ctgctttatc taccgtaaag accgcaatga aggtcgcgaa
    12001 cataaaggtc agtcccacga ccagagtcgc aatcatctgt tgttctgtgg tcgtaaagcc
    12061 cgccattttg aagatacgcg gcgcatagta catgatgatg ttcataccgg taaactgctg
    12121 catcgcctgc aacaacatac cgagaaacac agcacgacgg acgttacggt tgatcttaaa
    12181 cagtgcccaa ccgccttgtt ttaacttcag gctttcacga atttcgttga gttcttctcg
    12241 cgctttttcc gacgtatcgc gcagcatacg caacacttct tccgcctcaa tatgacgccc
    12301 cttttccgcc agccagcgcg ggctatttgg caggaagacc accagaataa tcagcaggac
    12361 tgctggtaaa gcaagaaccc ccaacattgc gcgccagtta ccgctataac tgaacgctgt
    12421 atcggataaa aacgccagca cgatgccgag tgtgaccatc aactggtaca tactgatcat
    12481 cttaccgcga acgttttcac ttgccatttc agaaagatac agaggagcgg tgtaagacgc
    12541 gatcccgaca gcaatgccca gcaccacacg agcggcgatt aacatctcta cgctggtcgc
    12601 aaaagcggac cctatagaac cgagtacaaa caggatggcc cccgccatca ggctgtattt
    12661 acgacccagg cggaacgaca gccaaccatt aaacagcgca ccaattgctg cgccgagcat
    12721 catgctacta accacccatt cctgcaaacg actggtcagc acaaagtgat cggtaatgaa
    12781 cggcaacgct ccggcgatta cgccgatatc aagaccaaat aacaatcctg cgaccgcagc
    12841 agctaccgaa acaaacatat tcatacgccg cgtatcccgc aaagaacgtg gcgttaaagc
    12901 agattccgta ttgatagtaa ccattttttc ctgccagcag agagtaagac atagtgaaaa
    12961 aatacgtgaa caactcacgc aggtgtcagg tcggaaacag cataaatatg gattaaattg
    13021 ctgcgacatg tcgttatgtg atggatattc caattttcaa attaag
//
LOCUS       DAZFYC010000137        12918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFYC010000137 DAZFYC010000000
VERSION     DAZFYC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.137"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..658
                     /locus_tag="ACHKW5_004316"
     CDS             <1..658
                     /locus_tag="ACHKW5_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO7513570.1"
                     /translation="GVAKLATLSDGTVFEPVNSFQKNQKTPARLQRQLSRKVKFSNNW
                     QKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPG
                     RNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKEN
                     RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQEPTEMIQATA
                     "
     gene            complement(698..1912)
                     /gene="ydcO"
                     /locus_tag="ACHKW5_004317"
     CDS             complement(698..1912)
                     /gene="ydcO"
                     /locus_tag="ACHKW5_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO7513571.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIFTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAGAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1965..2501
                     /gene="sutR"
                     /locus_tag="ACHKW5_004318"
     CDS             1965..2501
                     /gene="sutR"
                     /locus_tag="ACHKW5_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO7513572.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPVTPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2574..4535
                     /gene="rlhA"
                     /locus_tag="ACHKW5_004319"
     CDS             2574..4535
                     /gene="rlhA"
                     /locus_tag="ACHKW5_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO7513573.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4627..4857)
                     /gene="yncJ"
                     /locus_tag="ACHKW5_004320"
     CDS             complement(4627..4857)
                     /gene="yncJ"
                     /locus_tag="ACHKW5_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO7513574.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            5079..5233
                     /locus_tag="ACHKW5_004321"
                     /pseudo
     CDS             5079..5233
                     /locus_tag="ACHKW5_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            5279..5695
                     /gene="hicB"
                     /locus_tag="ACHKW5_004322"
     CDS             5279..5695
                     /gene="hicB"
                     /locus_tag="ACHKW5_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO7513575.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5774..7180
                     /gene="ydcR"
                     /locus_tag="ACHKW5_004323"
     CDS             5774..7180
                     /gene="ydcR"
                     /locus_tag="ACHKW5_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7513576.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7425..8570
                     /gene="ydcS"
                     /locus_tag="ACHKW5_004324"
     CDS             7425..8570
                     /gene="ydcS"
                     /locus_tag="ACHKW5_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO7513577.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8588..9601
                     /gene="ydcT"
                     /locus_tag="ACHKW5_004325"
     CDS             8588..9601
                     /gene="ydcT"
                     /locus_tag="ACHKW5_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7513578.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9602..10543
                     /gene="ydcU"
                     /locus_tag="ACHKW5_004326"
     CDS             9602..10543
                     /gene="ydcU"
                     /locus_tag="ACHKW5_004326"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7513579.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10533..11327
                     /gene="ydcV"
                     /locus_tag="ACHKW5_004327"
     CDS             10533..11327
                     /gene="ydcV"
                     /locus_tag="ACHKW5_004327"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7513580.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLATAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11349..12773
                     /gene="patD"
                     /locus_tag="ACHKW5_004328"
     CDS             11349..12773
                     /gene="patD"
                     /locus_tag="ACHKW5_004328"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO7513581.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3141 a         3176 c         3470 g         3131 t
ORIGIN      
        1 tggcgtggct aaactcgcca cgctgtcaga tggcacagtc tttgagcctg taaacagttt
       61 tcagaaaaac cagaagacgc cggcgagact tcagcgacag ttaagccgca aggtcaaatt
      121 cagcaacaac tggcagaagc agaaacgcaa aatacagcga ctgcattcct gtatcgcaaa
      181 tatccgcagg gactaccttc acaaagtcac aacgaccgtc agcaaaaacc acgcaatgat
      241 agtcattgag gatttgaagg tcagcaacat gtcaaagtca gcagcgggta cggtcagcca
      301 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
      361 gtatgaaatg cgccgccagc ttgaatacaa gcagctctgg cgtggcggtc aggtgcttgc
      421 tgttccgcca gcgtatacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      481 tcgtctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagcga acgccgatgt
      541 aaacggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagggatggt
      601 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      661 gtagcaggga tccacgtcct tcagggcgtg gaggatgtca atatcggttt ctgtcagcga
      721 ttaaattcaa caccacgtaa caaacgcctc cggcaattaa tccccagaac gcagaaccaa
      781 tcccgaccag cgtcaatcca cttgccgtta ccagaaatgc caccaccgcc gcgtcgcgct
      841 cacgctcatt atgcagcgcc tgatacaaac tgccgccaat ggtactcaac agcgccagac
      901 ctgccagcat ctggatccaa cttacgggca gggcagccat catcccggta atggcactac
      961 caaacagacc tgcgagcaaa tagaaaatgc ctgcaccggc agctgccagc caacgttgat
     1021 ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc gcaataccga
     1081 cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat acaattaatg
     1141 gcgaaacagg agttgaatat ccggctgctt tcattgctgc gatacccggt gcgttttgcg
     1201 atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca aacgaaaaat
     1261 caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg acaacgtcac
     1321 cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca tagcgcggcg
     1381 caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta aattgaccgt
     1441 ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc attgccgccg
     1501 caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg ccgcagagga
     1561 ctattaacgc gttggtgaaa ataaaaacgc cgatggcttc gttaagtgtt agtccctgca
     1621 atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca ggtacgcgat
     1681 accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc gtcatccagc
     1741 cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt attgccgctg
     1801 aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt ggaggaacag
     1861 aaaacagacg catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc atttgtccgt
     1921 tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa aatctcgctc
     1981 gctttttatc caccacactt aaacaactac gccagcagcg cggctggagt ctttcgcgac
     2041 tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt aatgagtcca
     2101 gtccgacggt cgcgacatta tggaaaattg ccaccgggtt gaacgtacca ttttcaacat
     2161 ttatttctcc gccgcagccc gttacgcctt cagtttatga tccacaacag cacgcgatgg
     2221 tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc tcgattcaga
     2281 tggcacccgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc gaacatgtcg
     2341 tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc cttaattgtg
     2401 gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt ggggagcaaa
     2461 ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa ctatttcgca
     2521 acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag aatgtgaccg
     2581 tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt gcccgcgaag
     2641 ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt gcccgtcata
     2701 atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat cgttatggtg
     2761 caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa cccgcgcaac
     2821 ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag gatatgggga
     2881 ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc gacattcgta
     2941 cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg ctggcgcgag
     3001 agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc attgaattct
     3061 ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct catgcgcaaa
     3121 cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca tacacattga
     3181 aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg aaagataacg
     3241 atcagacagc caacctcggc gcgctgattg atgctggcgt acgctccttc aagattgaag
     3301 ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc cagatgctgg
     3361 atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt actgaacatt
     3421 tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat tttgtgaatg
     3481 cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg ccggtgggtg
     3541 aagtattgaa agtggcgaaa gatcatctcg atgttgccgt caccgagcca ctggcaaatg
     3601 gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc aatacggtcg
     3661 agaaaaccag agaaaatcag taccgcgtct ggcccaatga aatgccagca gatttgcaca
     3721 aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag caggcactga
     3781 caaaaacctc cagcgaacgt cgggtggcgg tagacattga actgggcggc tggcaggaac
     3841 aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg ctggacgggc
     3901 agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat ggtctggcaa
     3961 aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg ggggcgctgt
     4021 ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg ctggatgctg
     4081 cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt tgctgatcct gcgccggttt
     4141 atccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg cgtgaatttt
     4201 atcatcgcta tggtgtgcag ctgattgacg cggcgtatga agcacatgaa gagaagggcg
     4261 aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg tgcccgaaac
     4321 aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt aacggcgatg
     4381 aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt ggcaaaatca
     4441 aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta agtccggatg
     4501 agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg gttactcaca
     4561 actcattgaa tctgcatgat attgcctgcc gggtaaggcg tctcgccgca tccggcaaca
     4621 aatgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa aatgctcgcg
     4681 caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg cttcatgacg
     4741 cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc ctgccatgag
     4801 ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg taaacatcct
     4861 gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgagg ttattattca
     4921 gttttgcaaa ttagcgcaaa gaaattctgg aatcttcctt cctgattttg cattgcattc
     4981 tgccgttgcg gcgatttagt gctattttcg agcaaattac acacagaggt aaacactaat
     5041 gtttactttg ttgatatact cagcggcagg gaggcgatgt gaaacaaagc gagttcagac
     5101 gttggctcga atctcagggc gtcgatgtag cgaatggccg caaccatttg aaactcaggt
     5161 ttcatgggag gcgcagtgtc atgccgcgtc actcctgcgt aaagcaatcc tgaaacaact
     5221 cggtttgagt taatcgccaa ttaaaaaggt taatgacatg cgagagacag tcgaaattat
     5281 gcgttatccc gtcactctta cacccgcgcc ggaaggcggt tatatggttt cttttgtgga
     5341 tatccctgaa gcgttgacgc agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga
     5401 tgctttactg accgcatttg atttttattt tgaagataac gagcttatcc cgttaccttc
     5461 gccattaaat agtcatgatc actttattga agtacctttg agcgtcgcct ctaaagtatt
     5521 gctgttaaat gcttttttac agtcagaaat cactcagcaa gagttagcca ggcgaattgg
     5581 caaacctaaa caggagatta ctcgcctatt taacttgcac catgcgacaa aaatcgacgc
     5641 cgtccagctt gcggcaaagg cgcttggcaa agagttatcg ctggtgatgg tttaattaca
     5701 gttaacgaaa agttgtcatt tttaacaact gatatagact gccgaatcat ctgcacataa
     5761 ttacgattcg ataatgaaaa aataccagca gcttgcagaa caattacgcg agcagattgc
     5821 gtcgggtatc tggcaacccg gcgatcgttt gccttcgttg cgtgaccagg tggcgctttc
     5881 tggcatgagc tttatgactg tcagccatgc ctatcagttg ctcgaaagtc tgggatatat
     5941 tatcgcgcga ccgcagtcgg gttattacgt tgcgccacag gcaataaaaa tgccgaaagc
     6001 gccagtcatt ccagtcactc gagatgaagc agtcgatatc aacacttata tttttgatat
     6061 gttgcaggcc agtcgcgatc cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg
     6121 acttttcccc ctccaacaac taaaccgctc gctggcgcag gtaagcaaaa ccgccacagc
     6181 gatgagcgtg attgaaaact taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg
     6241 tcgctatgcc ttacagggca tcaccatttc tcctgatgaa attgtcatta ctgccggggc
     6301 gttagaggca ttaaacctca gtttgcaagc ggtaactgaa ccgggcgatt gggtgatagt
     6361 agagaatcct tgtttctacg gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt
     6421 atcggtggcg acggatgtta aagaagggat cgatcttcag gcgctggaac tggcgttgca
     6481 ggattatccg gtgaaagcgt gctggctgat gactaatagc cagaacccac tcggatttac
     6541 cttaacgccg caaaaaaaag cacaactggt ggcgttactc aatcagtaca acgtaacgct
     6601 gattgaagat gacgtttaca gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa
     6661 agcgtgggat cgccacgatg gcgttttgca ttgctcttcg ttttcgaaat gtctggtgcc
     6721 tggttttcgt attggttggg tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca
     6781 gttgatgagt acgctttcca ccagctcacc gatgcaactt gcgctggtgg attacctttc
     6841 cacgcgccga tacgacgccc atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca
     6901 acgtgcctgg caggcactgc tgcgttatct gcctgcggaa gtgaaaattc atcataatga
     6961 cagtggttac tttctctggc tggagctccc cgagccgtta gatgccggcg aattaagcct
     7021 ggcggcactg acgcatcata tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa
     7081 ctggtcacgt tttttccgtt ttaataccgc gtggcagtgg ggagagcgtg aagaacaggc
     7141 ggtaaaacaa ttaggcaaac ttattcaaga acggctgtaa tagcgtttaa tttaattcct
     7201 cttagattgg gtaatatgaa tttcgaatag cagtcatatt tcctaactcc ttgactatac
     7261 tccagaagat aaccttacag acggcataat gcgcggtagc tcacaacctg attaaatttt
     7321 ctcaggggcg aaggtgtgcc tgcaagccgc cgtctatggt taaacaagga gatattttta
     7381 cggcacggcg gctgaacaat taattacgac aggagtaaga ccttatgagc aagacatttg
     7441 cccgcagcag cctgtgtgcg ctcagcatga caataatgac cgctcacgcc gccgaaccgc
     7501 ctaccaattt agataaaccg gaagggcgac tggatattat cgcctggccg ggatacatcg
     7561 aacgcggaca aactgataaa caatacgact gggtaacgca gttcgaaaaa gagacaggct
     7621 gcgcggtgaa tgtgaaaacc gccgcgactt ccgatgaaat ggtcagtctg atgaccaaag
     7681 ggggttacga tctggttacg gcatccggcg atgcctcgct gcgtttgatt atgggtaaac
     7741 gcgtgcagcc gattaatacc gcattgattc ccaactggaa aacgctcgat ccgcgcgtgg
     7801 ttaaaggcga ctggtttaat gttggcggca aagtttacgg cacaccttac caatgggggc
     7861 cgaacctgct gatgtacaac actaaaacct tcccgacgcc gccggatagc tggcaagtgg
     7921 tttttgttga gcaaaatctg ccggacggca agagcaataa aggccgcgtt caggcttatg
     7981 atggccctat ctacattgcg gacgctgcgt tgttcgttaa agccactcag ccgcagttgg
     8041 gcatcagcga tccgtatcaa ctcaccgaag aacagtacca ggcggtgctg aaagtgctgc
     8101 gcgctcaaca cagtttgatc catcgctact ggcatgacac taccgtgcaa atgagcgatt
     8161 tcaaaaacga gggtgtggtt gcttccagtg cctggcccta tcaggccaac gccctgaaag
     8221 ccgaaggcca gcctgttgct accgttttcc cgaaggaggg tgttaccggt tgggctgata
     8281 ccaccatgct gcatagcgaa gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt
     8341 cattaacgcc aaaagtgcag ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc
     8401 cggaagggtg taaagccagt ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta
     8461 actatttcga caaaatcgcc ttctggaaaa cgcctatagc agaagggggc aagtttgttc
     8521 cctacagtcg ctggacgcag gattacattg ccattatggg cggtcgctaa cttcgctggg
     8581 gtgctttatg acgtacgcag tggagtttga caacgtctcg cggttgtacg gtgacgtgcg
     8641 cgcagtagat ggcgtcagta ttgcgataaa agatggtgag ttcttctcta tgctggggcc
     8701 gtccggctcc ggcaaaacca cctgcctgcg cctgattgct ggcttcgaac agctttccgg
     8761 cggggctatc tctatctttg gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt
     8821 gaatactgtc tttcaggact acgcgctatt tccgcatatg tcgattcttg acaatgtcgc
     8881 ctatgggctg atggtcaaag gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc
     8941 gctggagaaa gtggcgttgg ggtttgtaca tcaacgtaaa ccgtcacaac tttctggtgg
     9001 tcagcgccag cgggttgcta tcgccagagc attggtgaat gaaccgcgcg tattgctgtt
     9061 ggatgaaccg ctcggcgcac tggatctcaa attgcgtgag cagatgcagc tggaactgaa
     9121 aaaactgcaa cagtctctcg gtatcacttt tatcttcgtt acccacgatc agggcgaagc
     9181 gttatcgatg tccgatcgtg tggcggtttt caataatgga cgcattgagc aggtcgattc
     9241 cccgcgcgat ctctatatgc gcccgcgcac gccgtttgtt gccggattcg tcgggacatc
     9301 caacgttttt gatggactga tggcagagaa actttgtggc atgacgggaa gcttcgccct
     9361 gcgaccggaa catatccgcc tcaacacccc tggtgaactg caggccaatg gcacgatcca
     9421 ggcggtgcaa tatcagggcg cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa
     9481 actgcttgtg agtcaggcca atatgacagg cgaagaactg cctgccacgc tcacgcccgg
     9541 acaacaggtg atggtttcct ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg
     9601 aatggcgatg aatgtattgc aatcaccttc acgtccaggt ctgggtaagg tgtccggttt
     9661 cttctggcgt aatccggggc tggggctgtt tttactgctg cttggcccgc taatgtggtt
     9721 tggcattgtc tatttcggct cgctgctgac actgttatgg cagggatttt atacttttga
     9781 cgatttcacc atgtcggtaa cgccggaact gacgctggcg aatatccgtg cgctgtttaa
     9841 tccggcgaat tacgacatca ttctccgcac gctgactatg gctgtggcag tcactatcgc
     9901 cagcgccatt ctggcttttc caatggcgtg gtatatggcg cgctatacca gcgggaaaat
     9961 gaaagctttt ttttatattg cggtaatgtt gccgatgtgg gcgagctaca ttgttaaagc
    10021 ctatgcctgg acgttattgc tggcaaaaga tggcgtggct cagtggtttt tacaacatct
    10081 tgggctggaa ccactgctga cggcgttcct tacattacct gcggtgggcg gaaatacgct
    10141 gtcaacttcc gggctggggc gctttctggt gtttctctat atctggttgc cgttcatgat
    10201 cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca ttgttgcagg cgtcggctga
    10261 tctcggcgca cgtccacgac aaacctttcg ctatgtggtg ctgccgctgg caatcccggg
    10321 tattgccgct ggctctatct ttaccttctc actcacactg ggcgatttta tcgtcccgca
    10381 gctggttggt cctccaggat attttatcgg caatatggtt tattcccagc agggggcgat
    10441 tggcaatatg ccgatggcgg cggcattcac cctggtgccg attgttctca tcgcactgta
    10501 cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc
    10561 tcaaactggc ggcctggggc ggcgtggttt tcctacattt tcccatcctg attatcgccg
    10621 cttatgcgtt taacactgaa gatgcggcgt ttagttttcc accgcagggc ctgacgctgc
    10681 gctggtttag cgtggcagca cagcgtagtg atattcttga tgccgtgaca ctgtcactta
    10741 aagtggcggc gctggcgaca ttaatggcgc tggtgttagg gacgctggca actgccgcgc
    10801 tgtggcgacg agactttttc ggcaaaaacg ccatttcgct gttactgctg ctgcccattg
    10861 cgctgccggg cattgtcact ggtctggcgc tattaaccgc ttttaaaacc atcaatctgg
    10921 agccgggatt tttcaccatc gtggtcggtc atgcgacttt ttgcgtagtg gtggtgttta
    10981 acaatgtcat cgcccgtttt cgccgcacct cgtggagtct ggttgaggcg tcaatggatc
    11041 ttggggccaa tggctggcaa accttccgct acgtagtgtt gccgaatctc agttcggcgc
    11101 tactggcagg aggaatgctg gcgtttgcct tgtcgttcga tgaaatcatc gttacgactt
    11161 ttacggcagg tcatgaacga acgttaccgt tgtggttgct caatcagctt gggcgaccgc
    11221 gtgatgtacc ggtaactaac gtggtggcac tgctggttat gttggtaaca accttgccga
    11281 tcctgggggc ctggtggcta acccgcgaag gcgacaatgg tcaataacca ctgatacagg
    11341 aatatgctat gcaacataag ttactgatta acggagaact ggttagcggc gaaggggaaa
    11401 aacagcctgt ctataatccg gcaacggggg acgttttact ggagattgcc gaggcatccg
    11461 cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa
    11521 ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg
    11581 gtcaggtttt tgccgaactg gagtcccgta attgtggcaa accgctgcat agtgcgttca
    11641 atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc
    11701 tgaatggtct ggcggcaggt gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc
    11761 cgttgggggt cgtggcttct atcgcaccgt ggaattatcc gctgatgatg gcagcgtgga
    11821 aactggctcc ggcgctggcg gcagggaact gcgtagtgct taaaccatca gaaattaccc
    11881 cgctgaccgc gttgaagttg gcagagctgg cgaaagatat cttcccggca ggcgtgatta
    11941 acgtactgtt tggcagaggc aaaacggtgg gtgatccgct gaccggtcat cccaaagtgc
    12001 ggatggtgtc gctgacgggc tctatcgcca ccggcgagca catcatcagc cataccgcgt
    12061 cgtccattaa gcgtactcat atggaacttg gtggcaaagc gccagtgatt gtttttgatg
    12121 atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt tggctattac aatgctggac
    12181 aggattgtac tgcggcttgt cggatctacg cgcaaaaagg catttacgat acgctggtgg
    12241 aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc gccggatgac gagtctacgg
    12301 agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg
    12361 cgaaagcgac agggcacatc aaagtgatca ctggcggtga aaagcgcaag ggtaatggct
    12421 attactatgc gccgacgctg ctggctggcg cattacagga cgatgccatc gtgcaaaaag
    12481 aggtatttgg tccagtagtg agtgttacgc ccttcgacaa cgaagaacag gtggtgaact
    12541 gggcgaatga cagccagtac ggacttgcat cttcggtatg gacgaaagat gtgggcaggg
    12601 cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg ggtcaatacc catttcatgc
    12661 tggtaagtga aatgccgcac ggtgggcaga aactttctgg ttacggcaag gatatgtcac
    12721 tttatgggct ggaggattac accgtcgtcc gccacgtcat ggttaaacat taagttagat
    12781 gttagtggct gttataggac ggccactatc gttttaggtt ctgagtttaa tggcggttaa
    12841 gtattagaat gattacatta ataactagcg taaaaattag gctaaatgtt tgatggcgat
    12901 ttttcatcct gtcacagt
//
LOCUS       DAZFYC010000138        12854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFYC010000138 DAZFYC010000000
VERSION     DAZFYC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.138"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..366)
                     /gene="ypeC"
                     /locus_tag="ACHKW5_004329"
     CDS             complement(40..366)
                     /gene="ypeC"
                     /locus_tag="ACHKW5_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO7513582.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(481..1737)
                     /locus_tag="ACHKW5_004330"
     CDS             complement(481..1737)
                     /locus_tag="ACHKW5_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO7513583.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGRPMMMVNRPKQQPPHDNV"
     gene            1941..2906
                     /gene="glk"
                     /locus_tag="ACHKW5_004331"
     CDS             1941..2906
                     /gene="glk"
                     /locus_tag="ACHKW5_004331"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO7513584.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHGNPGLLGSGAHLRQTLGHIL"
     gene            3125..3451
                     /locus_tag="ACHKW5_004332"
     CDS             3125..3451
                     /locus_tag="ACHKW5_004332"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO7513585.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            3473..4720
                     /locus_tag="ACHKW5_004333"
     CDS             3473..4720
                     /locus_tag="ACHKW5_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO7513586.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            4735..5820
                     /gene="ypdF"
                     /locus_tag="ACHKW5_004334"
     CDS             4735..5820
                     /gene="ypdF"
                     /locus_tag="ACHKW5_004334"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7513587.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            5820..6857
                     /gene="ypdE"
                     /locus_tag="ACHKW5_004335"
     CDS             5820..6857
                     /gene="ypdE"
                     /locus_tag="ACHKW5_004335"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7513588.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            6882..9377
                     /gene="ptsP"
                     /locus_tag="ACHKW5_004336"
     CDS             6882..9377
                     /gene="ptsP"
                     /locus_tag="ACHKW5_004336"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7513589.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDRDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9380..10237)
                     /locus_tag="ACHKW5_004337"
     CDS             complement(9380..10237)
                     /locus_tag="ACHKW5_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7513590.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10250..10984)
                     /gene="ypdB"
                     /locus_tag="ACHKW5_004338"
     CDS             complement(10250..10984)
                     /gene="ypdB"
                     /locus_tag="ACHKW5_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO7513591.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(10999..12696)
                     /gene="ypdA"
                     /locus_tag="ACHKW5_004339"
     CDS             complement(10999..12696)
                     /gene="ypdA"
                     /locus_tag="ACHKW5_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO7513592.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTHQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3036 a         3303 c         3494 g         3021 t
ORIGIN      
        1 agcgtcgcat caggcattgt gttccattgg cgaatgtgtt taatggcggt gtccatgtcc
       61 gcggcctttt ccgcgatgat cgtcacgatc gcgccagcct tcacgatagc cacgctcata
      121 cgctttacgc ttatcccagc cacgatgata gccattatca tgacgccacc agcggttttt
      181 acgccactca taattgcgat gccagtagtc acggtcgcgc cagtgaccac cgtcccagta
      241 attaccgtaa tgatcgcgat cgccaatttg taatttgatt gatggcagta gggtgatttc
      301 acctgcgttt gctgcaagcg gggtaaatgc catcagggcg gccgccagaa acagtgacct
      361 gaacattgtt attctccttc acgatcgaag ccgtcagcgg cctgttaacg caatattacg
      421 gggaggtaaa gccccgtatc atcgccataa ctcttaaatc ataaaggaga gcattttttg
      481 ctaaacgtta tcgtggggtg gctgttgctt cggacgattg accatcatca tcggcctacc
      541 tgctaataac agccatgccg ggagcatgac gatacagagc agcggtagca atgtggtatt
      601 gggtacaacg accgccgcca taaaaagact taaccagcca tcgcgtgtta ccaccagcac
      661 gatgccgaga atagcgcaag aaacggttat tgccgctggt acggcgggta cgtgctcatg
      721 cagcatcaac cctaatgcca cgccgacaaa cactgccggg aagattcgcc caccgcgaaa
      781 gccactggcg gcagcaacga ccagggcggc aagtttaatt accgccagca aaaagtaatc
      841 gctggtgctg aaagcctgat ttgccaccat ctgctgcatc tcatccagcc ctttaaacag
      901 cgaaactggt ccaccaataa cccccagaat accgagaata aatccgccaa tacccagcac
      961 gagcaccgga tttttcattt gattcatcat cgcgtgcaac cgtggtaagc accatacggc
     1021 gaccattcct gctgcgatgg caatcgccgc gacaattgca ccgctgagaa tatcggtcat
     1081 ttccatctgt ccgtaatgcg caatgggcag tgaaaaatgg ggatggaaaa ataatccggt
     1141 agtaagtgca ccagctgctg ccgccattaa tggcgcaaaa agacgatccc atagcggaac
     1201 ttcactactg ccatttaagg tttgcgaaaa tatcaacgcc gccgcaacag gtgtaccaaa
     1261 cagcgcgccg atggttccgg cagaggctaa aatagtccac tccattcggt tgacgcgcgg
     1321 taacagacga gcgccaatgg cgacggcaag ggcgatattg acggtcatga tcggatgttc
     1381 tggccccagg ctgacgccgc cagcaagacc gagaattaat gctacgataa gtcctggtag
     1441 cgcggaaggt ggaaccggtg cgccgatcag cggttcacag gcggggtctg gtccggcatg
     1501 accctggctg aagcggataa ccaaccccac cgcaatacct gttagcgtta atacaccgat
     1561 gatccagaaa ggtgaatcct gggctatccc cagagttccc ggcagtcgtt gccagagcaa
     1621 attctgtaat accgaggcga ttttcatcac cacaatcaga ataagactgg acgcaatccc
     1681 aattgccacg gcggggagcg ataataacaa catggttctg gctcgcggat ggagcatgtt
     1741 ctattcctta tgcggggtca gatacttagt ttgcccagct tgcaaaaagg catcgctgca
     1801 attggtgctg aaacgataaa gtaattgtgt gacccagatc gatatttaca gggagcctgc
     1861 ctttccggcg ttgttgttat gcccccaggt atttacagtg tgagaaagaa ttattttgac
     1921 tttagcggag cagttgaaga atgacaaagt atgcattagt cggtgatgtg ggcggcacca
     1981 acgcacgtct tgctctgtgt gatattgcca gtggtgaaat ctcgcaggct aagacctatt
     2041 cagggcttga ttaccccagc ctcgaagcgg tcattcgcgt ttatcttgaa gaacataagg
     2101 tcgaggtgaa agacggctgt attgccatcg cttgcccgat taccggtgac tgggtggcaa
     2161 tgaccaacca tacctgggcg ttctcaattg ccgaaatgaa aaagaatctc ggttttagcc
     2221 atctggaaat tattaacgat tttaccgctg tatcgatggc gatcccgatg ctgaaaaaag
     2281 agcatctgat tcagtttggt ggcgcagaac cggtagaagg taaacctatt gcggtttacg
     2341 gtgccggaac agggcttggg gttgcgcatc tggtccatgt cgataagcgt tgggtaagct
     2401 tgccgggcga aggcggtcac gttgattttg cgccgaatag tgaagaagag gccattatcc
     2461 tcgaaatatt gcgtgcggaa attggtcatg tttcggcgga gcgcgtgctt tctggccctg
     2521 ggctggtgaa tttgtatcgc gcaattgtga aagctgacaa ccgcctgcca gaaaatctca
     2581 agccaaaaga tattaccgaa cgcgcgctgg ctgacagctg caccgattgc cgccgcgcgt
     2641 tgtcgctgtt ttgcgtcatt atgggccgtt ttggcggcaa tctggcgctc aatctcggga
     2701 catttggcgg cgtgtttatt gcgggcggta tcgtgccgcg cttccttgag ttcttcaaag
     2761 cctctggttt ccgtgccgca tttgaagata aagggcgctt taaagaatat gtccatgata
     2821 ttccggtgta tctcatcgtc catggcaatc cgggtcttct cggttccggt gcacatttac
     2881 gccagacctt aggtcacatt ctgtaaatcc ttcctgttat atcgggaggt aactctcccg
     2941 ataatctttt aaatcataca gttaattcaa tttttctttg tgtcccctca caaggccgac
     3001 ctgcgtcaca cttccataca gcgggattaa ttctccagta aatgcattat ttgtctggta
     3061 acggcgattt gttttgcacg ttcataattt cactcgtcaa ctatcactga acgaggaatt
     3121 aacgatgagt aagaaactga ttgccttatg tgcctgcccg atgggcctgg ctcacacttt
     3181 tatggccgct caggcgctgg aagaagcggc ggtagaagcc ggttatgaag tgaaaattga
     3241 aacccagggc gcggacggca tccagaatcg cctgacggcg caggatatcg ccgaagcgac
     3301 catcatcatc cattccgtgg cagttacccc ggaagataac gaacgtttcg aatcacgcga
     3361 cgtttatgaa atcactttgc aggacgcaat taaaaacgct gtaggcatca tcaaagaaat
     3421 cgaagagatg attgcctctg aacaacagta atattcgaca gggataattg ttatggccat
     3481 taaaaaacgc agtgcaaccg ttgtgcctgg cgcatccggt gcggcagcgg cagttaagaa
     3541 tccgcaggcc tctaaaagca gcttctgggg tgaactcccg cagcatgtga tgtcagggat
     3601 ttcacgcatg gtgccgacct taattatggg cggtgtgatc ctcgctttca gccagctgat
     3661 tgcttatagc tggcttaaaa ttcctgctga gatcggcatc atggatgctc ttaatagcgg
     3721 gaaattctcc ggtttcgacc tctccttact gaaatttgcc tggctgtcgc agtccttcgg
     3781 cggcgtgttg tttggctttg ctattccgat gtttgccgct tttgtggcga actctatcgg
     3841 cggcaaactg gcgttcccgg ctggttttat cggcggcttg atgtctaccc agccgacgca
     3901 actgctgaac ttcgatccca gcactatgca atgggcgacc tcttcgccgg tgccgtccac
     3961 cttcattggt gcgctgatta tatctatcgt tgctggttac cttgtgaaat ggatgaacca
     4021 gaaaatccag ttaccagatt tcctgttagc gttcaaaacc acatttttgc taccgattct
     4081 ttccgccatt tttgtcatgc tggcgatgta ctatgtcatc accccatttg gtggctggat
     4141 caacggcggt atccgtaccg tgctgactgc cgcaggtgag aagggcgcgc tgatgtatgc
     4201 aatggggatt gccgcggcga ccgcaatcga ccttggtggc ccaatcaaca aagcggcagg
     4261 tttcgttgcc ttcagcttta ccaccgacca cgtattaccg gttaccgcgc gttctatcgc
     4321 tatcgttatt ccgccgattg gtctggggct ggcgaccatt atcgaccgtc gtttaaccgg
     4381 caaacgcctg ttcaacgctc aactttatcc gcagggtaag accgccatgt tcctggcgtt
     4441 tatggggatc agtgagggtg cgattccgtt tgcgctggaa agccccatca ccgccattcc
     4501 gtcgtatatg gtcggcgcta ttgtcggctc aaccgccgct gtctggctgg gcgcagtgca
     4561 atggttccca gagtccgcta tctgggcatg gccgctggtc actaacctgg gcgtctatat
     4621 ggcggggatc gcgctggggg cagtcataac tgcgctgatg gtggtgttcc tgcgtctgat
     4681 gatgttccgt aaaggcaaat tgttaatcga tagcctgtaa gaaggaaaat ccagatgaca
     4741 ttactcgctt cgctgcgcga ctggcttaag gcgcaacaac tggatgcagt gcttctctcc
     4801 tcacggcaga acaaacagcc gcatctgggg atctccaccg gatcaggcta tgtggttatt
     4861 agccgtgaaa gtgcgcacat tctggtggat tcgcgctatt acgttgaggt ggaagcccgt
     4921 gcgcaaggct accagctgca tttgcttgac gcgacgaaca cgcttaccac tatcgtcaat
     4981 caaatcattg ccgatgagca gttgcaaacg ctcggttttg aaggccagca ggtgagttgg
     5041 gaaaccgcgc accgctggaa gtctgaactc aatgcgaaac tggttagcgc cacgccggat
     5101 gtgctgcggc aaatcaaaac gccagaggag gtggagataa tccgcctcgc ctgtgggatt
     5161 gccgatcgcg gagcggagca tattcgccgc tttattcagg cggggatgag cgagcgcgag
     5221 atagccgctg aactggagtg gtttatgcgc cagcagggcg cagaaaaagc ctcttttgac
     5281 accattgtcg ccagcggctg gcgcggggcg ctgccgcacg gcaaagccag cgacaagatt
     5341 gttgcagcgg gcgagtttgt cactctcgat tttggtgcgc tgtatcaggg ctactgctct
     5401 gatatgacgc gcaccttgct ggtgaatggc gaaggggtga gcgccgaatc tcacccgctg
     5461 tttgacgtct atcagattgt cctgcaggca cagctcgcgg caatctctgc aattcgcccc
     5521 ggcgtgcgct gccagcaggt tgacgatgcc gcgcgccaag tgattaccga ggcaggtttt
     5581 agccactatt tcggtcataa caccggtcat gctatcggca ttgaagttca tgaagatccg
     5641 cgtttttcac cgcgggacac cacgacgcta cagccaggca tgttactgac cgtggagccg
     5701 gggatttatt tgccagggca agggggcgtg cgcatcgaag atgttgtgct ggttaccccg
     5761 caaggcgcag aagtgctcta cgccatgccg aaaacagtgt tgctcacggg agaggcataa
     5821 tggatttatc gctattaaaa gcgttgagcg aggcagatgc gatcgcctcc tcggaacagg
     5881 aagtgcggca gatcctgctg gaagaagcgg atcgcctgca aaaagaagtg cgatttgatg
     5941 gtctgggatc ggtgctgatc cgcctcaatg aatcgacagg tccgaaggtg atgatctgcg
     6001 cccatatgga cgaagtggga tttatggtgc gtagcatctc ccgcgaaggg gcaattgatg
     6061 tgctgccggt tggcaacgtg cgcatggctg cccgccagct gcagccggtg cgcatcacca
     6121 cccgtgaaga gtgcaaaatt ccaggcctgc ttgacggcga ccggcagggg aatgacgtca
     6181 gcgccatgcg cgtggacatt ggtgcgcgct cgtatgacga agtgatgcag gcgggaattc
     6241 gtccaggcga tcgcgtcacc tttgatacaa cttttcaggt tctccctcac cagcgggtga
     6301 tggggaaagc ctttgatgat cgcctcggtt gctatctgct ggtgacgtta ctacgtgagc
     6361 tgcacgatgc cgaactgcct gcggaggtgt ggctggtggc aagttccagc gaagaggtgg
     6421 gattacgcgg tgggcaaacc gctacccgcg cggtgtcgcc ggacgtcgcc attgtgctgg
     6481 ataccgcctg ctgggcgaaa aactttgatt atggcgcggc taaccatcgc cagattggta
     6541 acggcccgat gctggtgtta agcgacaagt cactgattgc gccgccaaaa cttaccgcct
     6601 ggatcgaaac cgtggcggct gaaattggcg taccgttgca ggcagatatg ttcagcaacg
     6661 gcggcacgga cggcggagcg gtgcacttaa ccggtactgg cgtacccaca gtggtaatgg
     6721 ggcctgccac ccgccacgga cattgcgcgg catcgattgc cgattgccgc gacattttgc
     6781 agatggagca acttttatct gcccttattc aacgtcttac ccgtgagacg gttgttcaac
     6841 tgacggattt cagatgatct cctgattaac ccggagcggt tatgttaacg attcaatttc
     6901 tctgtccttt gcctaacggt ctgcatgctc gtccggcgtg ggaacttaaa gaacagtgca
     6961 gccagtggca aagtgaagtc acctttatta accatcgcca gaacgcaaag gccgatgcga
     7021 aaagttcgct ggcgctgatt ggcaccggca ccctatttaa tgacagttgc agcctgaaca
     7081 ttagcggcag cgatgaagag caggcgcggc gcgtgctgga agagtacatc caggtgcgct
     7141 ttatcgacag cgacagcgtc cagcctacgc aggcagaact gacggcgcat ccgctgccgc
     7201 gttcattaag ccgccttaac ccggatttac tgtacggcaa tgtgctggca agcggcgtcg
     7261 gcgtgggtac gctgaccctg ttacaaagcg acagcctcga cagttatcgg gcaatccccg
     7321 ccagtgtgca agattccacc cggctggagc acagcctggc aacgcttgcc gagcaactga
     7381 accagcaatt gcgtgagcgt gacggcgaaa gcaaaactat cctcagcgcc catttgtcgc
     7441 tgattcagga tgatgaattt gcaggcaata tccgtcgcct gatgacagaa cagcatcagg
     7501 gactgggggc ggcgatcatc agcaatatgg agcaggtttg cgccaagctt tctgcttctg
     7561 ccagcgatta tctgcgcgaa cgtgttagcg acattcgcga tattagcgaa cagttgctgc
     7621 atatcacctg gccggaactg aagccgcgca acaatctggt gctggaaaaa ccgaccattc
     7681 tggtggctga agatttaacc ccaagccagt ttttgagcct tgatttgaaa aatcttgcgg
     7741 gcatgattct ggagaaaacc gggcgcacct cgcataccct gattctggcc cgcgcctcgg
     7801 cgatcccggt actgagcggc ctgccgctgg atgcgattgc ccgttatgcc gggcaaccgg
     7861 cggtgcttga cgcccagtgc ggcgtgctgg cgattaaccc gaatgacgcg gtgagcggtt
     7921 attatcaggt tgcgcagacg ctggcggata aacgccaaaa acaacaggcg caggctgccg
     7981 cgcagctggc ctattcccgt gataacaagc gtattgatat cgcggcgaat attggcaccg
     8041 ctctggaagc accaggcgcg tttgccaacg gcgcggaagg cgtcgggctg ttccgtaccg
     8101 aaatgctgta tatggatcgt gacagcgcgc cggacgagca ggagcaattt gaagcctacc
     8161 agcaggtgct actggcagcg ggcgacaagc cgattatctt ccgcacaatg gacatcggag
     8221 gcgataaaag cattccttat ctgaatattc cccaggaaga gaacccgttc ctcggctatc
     8281 gcgcggtacg tatttacccg gaatttgctg gcctgttccg cactcaacta cgggcgattt
     8341 tgcgcgctgc cagttttggc aacgcccagt tgatgatccc gatggttcac agcctcgatc
     8401 agatcttatg ggtgaagggc gagatccaaa aagcgatcgt tgagcttaag cgcgatggcc
     8461 tgcgtcatgc agagacgatt actctgggta tcatggtgga agtgccgtcg gtgtgttaca
     8521 tcatcgacca cttctgcgat gaggtcgatt tcttcagtat cggttctaac gatatgaccc
     8581 agtatctgta tgcggtcgat cgtaataacc cgcgcgtatc gccgctgtat aacccgatta
     8641 cgccatcgtt cctgcgcatg ttgcagcaga ttgttaccac tgcgcatcag cggggcaaat
     8701 gggtaggcat ttgcggtgaa ctgggcggtg aaagccgtta tctgccgcta ctgcttgggc
     8761 tgggcctgga tgagctgagt atgagtagcc cgcgtattcc ggcggtgaaa agccagcttc
     8821 gtcaactgga tagcgaggcg tgtcgggaac tggcgcgtca ggcatgtgaa tgccgcagtg
     8881 cgcaggaaat tgaagcgtta ctcaccgcct ttacgccgga agaagacgtt cgcccactgc
     8941 tggcgctgga gaatatcttt gttgatcggg attttagcaa taaagagcag gcgatccagt
     9001 tcctgtgcgg caacctcggc gttaacgggc gcactgaaca tccgtttgag ctggaagaag
     9061 atgtctggca gcgggaagag attgttacca ccggcgttgg ttttggcgta gcgatcccgc
     9121 acaccaaatc tcagtggatc cgtcattcca gtatcagcat tgcccggctg gcgaaaccga
     9181 ttgactggca gtcagaaatg ggcgaagtcg aactggtgat catgctgacg ctgggtgcta
     9241 acgaagggat gaatcatgtg aaagtcttct cgcagctggc gcgtaaactg gtgaataaaa
     9301 acttccgcca gtcgttgttt gccgcgcaag atgcacaaag tatcctgacg ctgctggaaa
     9361 cagaattaac cttctgacgt tagccctgaa aacgggcgct gtactctccc ggcgtcagac
     9421 caaactgacg ccggaaaacg cgacaaaaat agtcgctatc cggaaaaccg caacgctgtg
     9481 ccacttcatg aattgacaga tgatatttct gcaaaatcat cctcgccttc gccattcgca
     9541 cccaacgcac atactcgata aaacgcatcg ttccatgctg agcaaacagt tttgacagat
     9601 gattgggcgt gatattaaaa aactgcgcca cgctctcgcg ggtgagcggc tgggcataat
     9661 tatcctgaac ccagttacaa atgctgtgat agagaaaatc gccgcgcggc tgcgaattgc
     9721 ccggcggcat attcaccacg ttacggcaaa gatgcagcaa acttaacacc agtggctgaa
     9781 tgatattctg ctccagcggc gaacggctaa gatgggtgag ggcggtaagc atcgcttcac
     9841 cttcaccgcg ttgcaggctg ggaagctgga tctggcgagc aggatgcaac aacgacgtgg
     9901 tgcggctatc gtaaaacgat aacccaagcc aggtcggtgc gaacaccagg cttaacagca
     9961 tcaccggttt gttgttgatc ggtaaattag ccgcgcgtgc cggaataaac agcatttctc
    10021 cttccaccat tcgctgctgc tttgcttcca gcaggttgcc gtattcgccg cgcagcacga
    10081 tatccagacg gggaaaatca atacataaac tgcccaccgg taacgaggca ggctggctgg
    10141 caaaccagac gcgccccagt agttgcgggt taagcaccag gccgctgaac agatcggcga
    10201 aaaattgttg atcggcgggc aagcctggtg ctttcatgaa agttcccgat taaagatgca
    10261 ttaactggcg aaattctttc actttgctgc ggctgaccgg cacttcaaaa tccagatctt
    10321 tcagtcgcag aatgtaggtg ttattaaacc acggttcgat ttcgcgtatt ttgttcagat
    10381 tgacacaaaa tgagcgatgg cagcggaaaa aatgcgacgg cggcagtttg ctgcaaaatt
    10441 cggtaatgtt catcggcatt acgtaggatt cacgccgcgt atagacaaag gtcattttct
    10501 cgtgcgcttc ggcgtaatag atatcgttaa ttggcgtgac gattattcgc tcatctttca
    10561 ccagattaat ggtgtcattc tcacgcgtta ccgtcgcggc aggcgtactg ctggtctgct
    10621 gttgttgcca ggccgcttcc agtttttgca gcatcccggt aatacgtgac tcctgatacg
    10681 gtttgagaat gtagtcaaac gcctccagtt caaacgcttc taccgcatgt tctttccacg
    10741 cggtgatgaa cacaataaac ggtttatggg cgaactggct gatgttttgc gccagcaaca
    10801 cgccatccag cgacggaata ttgatatcca gaaaaatggc gtcgacgcgg ttatgctgca
    10861 aaaacttcaa cacgtccaga ccgtcgtcaa aggtgccgac aatctccatc tggctgtgct
    10921 ctttaattag ccagctcagt tcctgttgtg ccaggaattc gtcttcaaca atgatgactt
    10981 tcacaatatc actccggctc aaagcaataa cgtagcctgt gaggcgactg gggtgcgttg
    11041 gttaggaatg taaaacgcaa tttccgttcc tggttccagg cggcggatat gcagcccctc
    11101 gccatacaat aacttcacgc gatgatgaac attcagcagg ccaattttat tgcccggcat
    11161 ttcattcgct tcgacccgct caatcacctt tggatcgatg ccgtgcccgg tatcccgcac
    11221 cgcaatgcgt acccgattgc cgcactctgc aacgctgatg gtgacaacgc ctttaccttt
    11281 gcaaggctga ataccgtgga caatggcatt ctccaccaac ggctggatca gcaggctggg
    11341 aatgcagcaa ttcacctctt catcaatatc atagataacc gtcagcttgt cgccaaagcg
    11401 ggcctgctca atggcaatat agtctttaat ttgatacagc tcttttttga tatcgatttg
    11461 ctcatcgtct tttaattcaa tgttatagcg cagataacgc gacagattaa agatcagctg
    11521 gcgagcagta tccggattca gacggattga tgatgaaata gcgttcaaag cgttaaacag
    11581 gaaatgggga ttaattttgc tttgcagggc gcgaagctct gccttatttg ccatttcacg
    11641 tagctgctcg gcgcgtgaaa cctccagttg ggtggagatg atttgcgaca gaccgaccgc
    11701 catttcctgt aatgacgagg tgatctgatg cgcgtggcag tagtaaattt tcagcgttcc
    11761 ggtcacaacg cctttctccc acaatgggat caccagcatg gaatgaatct ctggtgtgcg
    11821 gtgggcttca tcattgtttt taatgatgat ttttccgtaa ttcatcgcct gatgggtagt
    11881 cgggctaatg aagtcatcgc cattctgata gttatgttca cccacgccaa cataggccag
    11941 cacatgatcg gtattggtaa tcgccaccgc atcggcgtga atgtcatcgc gaataatttc
    12001 acaaactttg cgtaatgact cgctattgac atggcgaaac agaggtagcg ttttgttggc
    12061 gatatccagc gccagcttgg cctgccgcgc ggcgctggcc tctttttcgc cctcaacgct
    12121 ttgcaccaga agcacaataa agccgataca gacgctaccg aggatcattg gaatgccgat
    12181 tttagagacg atatcgatcc ccaacgcggt agttggtgcc cagacaatta ccagaatcat
    12241 ggtcaacgtc tcacacaaca tgccgccgag aataccgacg cgccagcgct gtgctttggg
    12301 gattttcagg tttatccagc ctgatataca acccgccaga atgctggtga taaagcaggg
    12361 gatcgccgtc acgccgccga tatcaattaa ataccggtga atacccgcaa tcacgccggt
    12421 aatgatgcct acccacgggc caaacagaat cccgccggac atcaccgcga taatacgtac
    12481 gttcaccagc gagccttcta cgggaacgcc ggaccaggtg ctgaacaggg cgaacagcga
    12541 gaaaatggcg gtaacggcga gcaattcttt tggggagtgc gccgacttgt gcaacagttc
    12601 gcgaaacagg cggatacgga tgagaaagaa caggcagata agcattaacg ctgcccgatc
    12661 gaagaccgcc agcagcatgt tgaatatttc gtgcacgggt gactctgaat aaggattaaa
    12721 cctctatgat aggtaacctg aaggctgatg accagcaggc cgtttttgag gaaaaaccat
    12781 aaatttaaaa ggggattgaa agataaatgg tgtattagca atgtatctct tgataatgca
    12841 cttctggctg gtaa
//
LOCUS       DAZFYC010000139        12639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFYC010000139 DAZFYC010000000
VERSION     DAZFYC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.139"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..165
                     /locus_tag="ACHKW5_004340"
                     /pseudo
     CDS             79..165
                     /locus_tag="ACHKW5_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(162..410)
                     /locus_tag="ACHKW5_004341"
     CDS             complement(162..410)
                     /locus_tag="ACHKW5_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7513593.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            630..2216
                     /gene="prfC"
                     /locus_tag="ACHKW5_004342"
     CDS             630..2216
                     /gene="prfC"
                     /locus_tag="ACHKW5_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO7513594.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            2609..3214
                     /gene="osmY"
                     /locus_tag="ACHKW5_004343"
     CDS             2609..3214
                     /gene="osmY"
                     /locus_tag="ACHKW5_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO7513595.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            3341..3502
                     /gene="ytjA"
                     /locus_tag="ACHKW5_004344"
     CDS             3341..3502
                     /gene="ytjA"
                     /locus_tag="ACHKW5_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO7513596.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            3624..4697
                     /gene="yjjU"
                     /locus_tag="ACHKW5_004345"
     CDS             3624..4697
                     /gene="yjjU"
                     /locus_tag="ACHKW5_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO7513597.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            4694..5476
                     /gene="yjjV"
                     /locus_tag="ACHKW5_004346"
     CDS             4694..5476
                     /gene="yjjV"
                     /locus_tag="ACHKW5_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7513598.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAA
                     NFARVLALAENYQPLYAALGLHPGMLEKHSDASLGQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(5706..6569)
                     /gene="yjjW"
                     /locus_tag="ACHKW5_004347"
     CDS             complement(5706..6569)
                     /gene="yjjW"
                     /locus_tag="ACHKW5_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO7513599.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIGGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGH
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(6541..8091)
                     /gene="yjjI"
                     /locus_tag="ACHKW5_004348"
     CDS             complement(6541..8091)
                     /gene="yjjI"
                     /locus_tag="ACHKW5_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO7513600.1"
                     /translation="MPTSHENALQQRCLQIVTSPVLSPEQKRHFLALEAENNLPYPAL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFARTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IVARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            8349..9128
                     /gene="deoC"
                     /locus_tag="ACHKW5_004349"
     CDS             8349..9128
                     /gene="deoC"
                     /locus_tag="ACHKW5_004349"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO7513601.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            9206..10528
                     /gene="deoA"
                     /locus_tag="ACHKW5_004350"
     CDS             9206..10528
                     /gene="deoA"
                     /locus_tag="ACHKW5_004350"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO7513602.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            10580..11803
                     /gene="deoB"
                     /locus_tag="ACHKW5_004351"
     CDS             10580..11803
                     /gene="deoB"
                     /locus_tag="ACHKW5_004351"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7513603.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            11860..12579
                     /gene="deoD"
                     /locus_tag="ACHKW5_004352"
     CDS             11860..12579
                     /gene="deoD"
                     /locus_tag="ACHKW5_004352"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO7513604.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3015 a         3240 c         3406 g         2978 t
ORIGIN      
        1 aatctgagca gtctggagaa tttgaaatcg gttagggatt tcatactaga ataataggat
       61 ccgcggcacg tcgtatgcaa gaacgtgcca cggctggctg gcgaactttc gatagtgcga
      121 gtattgaatg atttccagcc gttaccgatt ttacgtgtta attagtgaac aaaccactcg
      181 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
      241 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
      301 tcgtcataac gcttactgat gcgccgagtt aattcttcct ttaacgcatc cacagcaccg
      361 tttggcattt tagtcatttt ctctttggct atgcagatct caatacgcat aaaaatccct
      421 ctacactgtg tttgtataca gtgttatttt taactgtatg aataaacagt gtcaatagaa
      481 caaagattgc tgcatgtaca aatgttcttt ggctacaaat acacctactg atcatgttct
      541 gctatgctct gatacctcta aaatgtacaa tcagcggcat gaatttttct ttgtacatat
      601 gattgtacat aatcaaagag acaacaatta tgacaagcct tttggccgca gaggtagcga
      661 aaagaagaac ctttgcaatt atttctcacc cggacgccgg taagactacc atcaccgaga
      721 aggtgctgct gttcggacag gccattcaga ccgccggtac agtaaaaggc cgtggttcca
      781 accagcacgc taagtcggac tggatggaga tggaaaagca gcgtgggatc tccattacca
      841 cgtctgtgat gcagtttccg tatcacgact gcctggttaa cctgctcgac accccggggc
      901 acgaagactt ctcggaagat acctatcgta ctctgacggc ggtggactgc tgcctgatgg
      961 ttatcgacgc tgcaaaaggt gttgaagatc gtacccgcaa gctgatggaa gttacccgtc
     1021 tgcgcgacac gccgattctc acctttatga acaaacttga ccgtgatatc cgcgacccga
     1081 tggagctgct cgatgaagtt gagaacgagc tgaaaatcgg ctgtgcaccg atcacctggc
     1141 cgattggctg cggcaagctg tttaaaggcg tttaccacct ttataaagat gaaacctatc
     1201 tttatcagag tggtaaaggc cacaccattc aggaagtccg cattgttaaa gggctgaata
     1261 acccggatct cgacgctgcg gttggtgaag atctggcaca gcagctgcgt gacgaactgg
     1321 aactggtgaa aggcgcgtct aacgagttcg acaaagaatt gttccttgcg ggcgaaatta
     1381 ctccagtgtt ctttggtact gcgctgggta acttcggcgt tgatcatatg ctggatggcc
     1441 tggtggagtg ggccccagcg ccgatgccgc gtcagactga tacccgtacc gtagaggcga
     1501 gcgaagacaa atttaccggc ttcgtattta aaattcaggc caacatggac ccgaaacacc
     1561 gcgaccgcgt ggcgtttatg cgtgtggtgt ccggtaaata tgaaaaaggc atgaagctgc
     1621 gccaggtgcg tactgcgaaa gatgtggtga tctccgacgc gctgaccttt atggcgggtg
     1681 accgttcgca cgttgaagaa gcgtatcctg gcgatatcct tggtctgcac aaccacggca
     1741 ccattcagat cggcgacacc tttacccagg gtgagatgat gaagttcacc ggtattccga
     1801 acttcgcgcc agaactgttc cgtcgtatcc gcctgaaaga tccgctgaag caaaaacagc
     1861 tgctcaaagg gctggtacag ctttccgaag agggcgcggt gcaggtgttc cgtccgatct
     1921 ccaacaacga tttgatcgtt ggcgcggttg gtgtgctgca gtttgatgtg gtggtagcgc
     1981 gcctgaagag cgaatacaac gttgaagcag tgtatgagtc agtcaacgtt gccaccgccc
     2041 gctgggtaga atgtgcggac gcgaagaaat tcgaagagtt caagcgtaag aacgaaagcc
     2101 aactggcgct tgatggcggc gataacctcg cttacatcgc taccagcatg gtaaacctgc
     2161 gcctggcgca ggaacgttat ccggacgttc agttccacca gacccgcgag cattaattct
     2221 tgcctccagg gcgcggtagc cgctgcgccc tgtcaatttc ccttccttat tagccgctta
     2281 cggaatgttc ttaaaacatt cacttttgct tatgttttag cggatatccc gagcggtttc
     2341 aaaattgtga tctatattta acaaagtaat gacatttctg acggcgttaa ataccgttca
     2401 atgcgtagat atcagtatct aaagccgtct attgtcattc taccgatatt aataactgat
     2461 tcagaggctg taatggtcgt tattcatcac ccatcgcttt tgtgatggcg accattgact
     2521 tctgtagagg gtgaagtctc tccctattca gcaatgcaac ctcgtgttgc caggctcaaa
     2581 ttacgagcaa acatacagga ataaatcgat gactatgaca agactgaaga tttcgaaaac
     2641 tctgctggct gtaatgttga cctctgccgt cgcgaccggc tctgcctacg cggaaaacaa
     2701 cgcgcagact accaatgaaa gcgcagggca aaaagtcgat agctctatga ataaagtcgg
     2761 taatttcatg gatgacagcg ctatcaccgc gaaagtgaag gcggccctgg tggatcatga
     2821 caacatcaag agcaccgata tctctgtcaa aaccgatcaa aaagtcgtga ccctgagcgg
     2881 tttcgttgaa agccaggccc aggccgaaga ggcagtgaaa gtggcgaaag gcgttgaagg
     2941 cgtgacctct gtcagcgaca aactgcacgt tcgcgacgct aaagaaggct ctgtgaaggg
     3001 ctacgcgggt gacaccgcca ccaccagtga aatcaaagcc aaactgctgg cggacgatat
     3061 cgtcccttca cgtaaagtga aagttgaaac caccgatggc gtggtccagc tctccggtac
     3121 cgtagattct caggcacaaa gtgaccgtgc tgaaagtatc gccaaagcgg tagatggtgt
     3181 gaaaagcgtt aaaaatgatc tgaaaactaa gtaattcgtc gtaattcgtc ctcccgaact
     3241 ttgtcgggag gcgcgatgtg caccacacta aaaatatcgc attgagtgat tttcacgctc
     3301 atatcaagcg gtgacattaa ctatggtaaa ggagacgctt atgtttcgtt ggggcatcat
     3361 atttctggtt atcgcgttaa tcgccgccgc acttgggttt ggtggtctgg ccggtactgc
     3421 tgcaggcgca gctaaaattg tctttgtcgt cgggattatt ctgttcctgg tgagtttgtt
     3481 catgggccga aaacgaccct agatttcaga ccattaagaa aattttatcc aaagccagtc
     3541 cagcggactg gcttttgcgg ttttagcgaa taataaattg cgttactttg tccttctttc
     3601 acaacaaaaa caggaaggca gaggtggggc agcgaatacc tgtaacgctt ggtaatattg
     3661 cgccgttgtc gctgaggccg ttccagcctg gacggattgc tctggtgtgc gaaggcggcg
     3721 gacagcgtgg aattttcacg gctggcgtgc tggatgagtt tatgcgcgcg cagtttaatc
     3781 ctttcgatct ttatctcggc acatctgccg gggcgcagaa cctctcggcg tatatctgca
     3841 atcagcccgg ttacgcgcgc aaagtcatca tgcgctatac cacaaaacgc gaatttttcg
     3901 atccattgcg ctttgtccgt ggaggaaatc ttatcgatct cgactggctg gtggaggcca
     3961 ccgcaagcca gatgccgttg caaatggata ccgcagcgcg gctatttgac agcggcaaat
     4021 cgttttatat gtgcgcttgt cgtcaggatg actatgcgcc gaattacttt ttgccaacca
     4081 aacagaactg gctggatgtg attcgcgcct ccagtgcgat acctggcttt tatcgtagcg
     4141 gagtgtcgct ggaaggcatt aactacctgg atggcgggat cagtgatgcg attccggtta
     4201 aagaggcggc aaggcagggc gctaaaacgt tggtcgtcat ccgcaccgtg ccgtcgcaaa
     4261 tgtactacac gccgcagtgg ttcaaacgca tggaacgctg gctgggtgac agtagcctgc
     4321 agccgctggt caatctggtg cagcatcatg aaaccagcta tcgtgagatt cagcaattta
     4381 ttgagaaacc accgggccag ctgcggatat tcgaaattta tccgccgaag ccattgcata
     4441 gtattgcgct cggcagtcgg attccggcgc tgcgtgaaga ctataaactt gggcgtttat
     4501 gtgggcgtta ttttctggca acggtcggta agttattgac tgaaaaagcg ccgcttaccc
     4561 gccatctggt gccagtggtg acgccggaat cgatagtcat tccgcctgcg ccagtcgcta
     4621 acgatacgct ggttgccgaa gtgagcgacg ctccgcaggc gaacgacccg acatttaaca
     4681 atgaggatct ggcttgattt gccgttttat cgacacccac tgccattttg atttcccgcc
     4741 gtttagtggt gatgaagagg ccagcctgca acgcgcggcg caagcgggcg taggcaagat
     4801 cattgttccg gcaaccgagg cggcaaattt tgcccgtgtg ttggcattag cggaaaatta
     4861 tcaaccgctg tatgccgcat tggggttgca tccggggatg ttagaaaaac atagcgatgc
     4921 atcgctgggg cagttacagc aggcgctgga aaggcgtccg gcgaaggtgg tggcggtggg
     4981 ggagatcggt ctggatctct tcggcgacga tccgcaattt gagaggcagc agtggttact
     5041 cgacgaacaa ctgaaactgg cgaaacgcta cgatctgccg gtgatcctgc attcacggcg
     5101 cacacacgac aaactggcaa tgcatcttaa acgccacgat ttgccacgca ctggcgtggt
     5161 tcatggtttt tccggcagcc tgcaacaggc cgagcggttt gtgcagctgg gctacaaaat
     5221 cggcgtaggc ggtactatca cctatccacg cgccagtaaa acccgcgatg tcatcgcaaa
     5281 attaccgctg gcatcgttgt tgctggaaac cgacgcgccg gatatgccgc tcaacggctt
     5341 tcaggggcag gctaaccgcc cggaacaggc cgcccgtgtg tttgccgtgc tgtgcgagtt
     5401 acgcccggaa cccgcggatg agattgccga agtgttgctt aataacacgt atgcggtgtt
     5461 taacgttcgt gggtagtctg cccttcgtaa gccggataag gcgttcacac cgcatccggc
     5521 agtcgacgca caatgcctga tgcgacgctg acgcgtctta tcatgcctac aggtctgtgc
     5581 cgaaccgtag gccggataag gcgttcacgc cgcatccggc agtcggcgca caatgcctga
     5641 tgcgacgctg acgcgtcttt tcatgcctac aagtctgtgc cgaaccgtag gccacatccg
     5701 gcatgtcaca aatacagcgc cggaaatatc aaccggctca ccccgcgctc ccgtaacgca
     5761 tcagccaact gctcaacgtc ttccggcgtg gcactcgccc agctttgcgc ctcgccatac
     5821 acgccgtggg catgaaacgc gttcaggcgt accggaacat cgccaagtcc cttgataaac
     5881 gccgccagtt cttcgatgtg ttgcaaataa tccacgtggc ctgggattac cagcaaacgc
     5941 agttccgcca gcttaccgcg ctctgcaagc aaacagatgc tgcgcttaat ctgctgatta
     6001 tcgcgtccgg ttaattgctg atgacattcg ctcccccacg ctttgagatc gagcattgcg
     6061 ccgtcgcaca ccggaagcaa tttttcccag ccggtttcgc tcaacatgcc attactgtcc
     6121 accagacagg tgaggtggcg cagttgcgga tcatttttga tagcagtaaa cagcgccacc
     6181 acaaacggca gctgggtcgt ggcttcgccg ccgcttaccg ttatccctcc aataaacagc
     6241 accgctttgc ggatatggct aagcacctca tccacgctca tggattgcgc cattggtgtg
     6301 gcatgttgcg gacacatctt caggcaggta tcgcactgct cgcagactac agcgttccac
     6361 gccactttgc cgtcaacaat ctgcaacgcc tgatgcggac actgtggcac gcactccccg
     6421 caatcattgc aacgtcccat cgtccacgga ttgtgacagt ttttgcagcg cagattgcag
     6481 ccctgcaaaa acagagccag acgactgcct ggcccgtcaa cgcaggagaa ggggataatc
     6541 ttactgacta aagcgcatct gctgttcatg gcttatcacg cgcggctggc gttccagaat
     6601 acgagtgttg cgtgcggcct cttcgcccag ccaggtggta ttggtacgtg agccttcggc
     6661 acgatatttt tccagatccg acaaacgcac catataacca gtaacgcgaa ccagatcgtt
     6721 accgctgaca ttggcggtaa attcacgcat tccggcttta aaggcaccga ggcaaagctg
     6781 taccagcgcc tgcggattac gtttgatggt ttcgtcgagc gtcagaatgt cgctgattcc
     6841 agagtaataa taagcatgat gcggggcgac ggtttgcaga tgggtaatcg gatccggttc
     6901 atcaccatat ggcaagcgtg cgccaggcgt ggtgccgata tcggaactga tccccgactg
     6961 tgcgtgtagc atggcgcgtt tttgccagcc atatttcacg ggggtattgg cgacaaactc
     7021 cgccagttgt gcgctgatgc gataacccac ttcatttgca gcggcttctt taccgtaacg
     7081 tgcgacaatt ccttctttct cacacagtaa gttaactgct tccgccagtc cgtacatgcc
     7141 aaacattggc acaaaacgtt cagggttaat cagcccttct ttcaccagga agctattctc
     7201 aaagaagtgt gattgttgat agaggaattc acaccgcgca tcgatgatgg cgatctgctg
     7261 ctggcagtag tgcggtagag tgcgcgcaaa gaagtcatcc agcgattcgc tgcgctcggc
     7321 aatggctttc aggttaaggc gtaccagcgt gctgccacca cccgccagcg gcagtgagtt
     7381 gtaacagctc acaatcccgt agcccccttt tgtgaaaatt ttatcatgca cgggaccgtt
     7441 ggcgatgtgc ggtttgctac attcacagat gttcttcgcc acttccagca gcaggtcatc
     7501 aggggtgatt tcaggatcgt agataaaggt caggttcggt gaaacctgct tcaactctgc
     7561 gtctgcacgt aagattgcgc gggtaatggg cgaatcagac gggccgatat tggcgtgcat
     7621 aaaggcatct ggcagggttc tgtcgaggta acgccagaaa cgttttattc gaatatctat
     7681 ctcgtcttgt gttagaattc taacatacgg ttgcaacaac gcatccagtt gccccaggta
     7741 gaccggcatc gatgtgaccg acggtacgtg gtggtaaaga atggtcagca gagagagtgc
     7801 gtcatcaaga tctttcgcgc cttccagctc cagccattcg gaaccgttcg ccagaaaacg
     7861 ggcgtaatcg ggtaagacat agcgcggttt gtacggcgca tgaccttcaa acatatcgca
     7921 gattacaccc tcatccagcg cgcggcgggc ttcggcaggc aacgccgggt aaggcagatt
     7981 gttttctgct tccagtgcca gaaaatggcg cttctgctcc gggctaagca ctgggctggt
     8041 gacaatttgc aggcaacgtt gttgcagtgc attttcatga gaagtgggca tcttcttttc
     8101 cttttatgcc gaaggtgatg cgccattgta agaagtttcg tgatgttcac tttgatcctg
     8161 atgcgtttgc caccactgac gcattcattt gaaagtgaat tatttgaacc agatcgcatt
     8221 acagtgatgc aaacttgtaa gtagatttcc ttaattgtga tgtatatcga agtgtgttgc
     8281 ggagtagatg ttagaatact aacaaactcg caaggtgaat tttattggcg acaagccagg
     8341 agaatgaaat gactgatctg aaagcaagca gcctgcgtgc actgaaattg atggacctga
     8401 ccaccctgaa tgacgacgac accgacgaga aagtgatcgc cctgtgtcat caggccaaaa
     8461 ctccggtcgg caataccgcc gctatctgta tctatcctcg ctttatccca attgctcgca
     8521 aaaccctgaa agagcagggc accccggaaa tccgtattgc tacggtaacc aacttcccac
     8581 acggtaacga cgacatcgaa atcgcgctgg cagaaacccg tgcggcaatc gcctacggtg
     8641 ccgatgaagt tgacgtggtg ttcccgtacc gcgcgctgat ggcgggtaac gagcaggttg
     8701 gttttgacct ggtgaaagcc tgtaaagagg cttgcgctgc ggctaacgta ctgctgaaag
     8761 tgatcatcga aaccggcgaa ctgaaagacg aagcgctgat ccgcaaagcg tctgaaatct
     8821 ccatcaaagc gggtgctgac ttcatcaaaa cctctaccgg taaagtggcc gtgaacgcga
     8881 cgccggaaag cgcgcgcatt atgatggaag tgatccgtga tatgggcgta gaaaaaaccg
     8941 ttggtttcaa accggcaggc ggcgtgcgta ctgcggaaga tgcgcagaaa tatctcgcca
     9001 tcgccgatga actgttcggc gctgactggg ccgatgcgcg tcactaccgc tttggcgctt
     9061 ccagtctgct ggcaagcctg ctgaaggctc tgggccacgg cgacggtaag agcgccagca
     9121 gctactaagg cttgatttgc cggatgacgc agcgatgctc atccggttcc ccgattacct
     9181 cgtctctttt ccacgggagg ttaccttgtt tctcgcacaa gaaattattc gtaaaaaacg
     9241 tgatggtcat gcgctgagcg atgaagaaat tcgtttcttt atcaacggta ttcgcgacaa
     9301 cactatctcc gaagggcaga ttgccgccct cgcgatgacc attttcttcc acgatatgac
     9361 aatgcctgag cgtgtctcgc tgaccatggc gatgcgagat tcaggaaccg ttctcgactg
     9421 gaaaagcctg catctgaatg gcccaattgt tgataaacac tcgaccggcg gcgtcggcga
     9481 tgtgacttcg ctgatgttgg ggccgatggt cgcagcctgc ggcggctata ttccgatgat
     9541 ctccggtcgc ggcctcggtc atactggcgg tacgctcgac aaactggaat ccatccctgg
     9601 cttcgacatt ttcccggatg acaaccgttt ccgcgaaatt attaaagacg tcggcgtggc
     9661 gattatcggc cagaccagct cactggctcc ggcggataaa cgtttctacg cgacccgcga
     9721 tattaccgca accgtggact ccatcccgct gatcaccgcc tctattctgg cgaagaaact
     9781 ggcggaaggt ctggatgcgc tggtgatgga cgtgaaagtg ggaagcggtg catttatgcc
     9841 gacctacgaa ctttctgaag cccttgccga agcgattgtt ggcgtagcta acggcgctgg
     9901 cgtgcgtacc accgcgctgc tcaccgatat gaatcaggta ctggcctcca gtgcaggtaa
     9961 cgcggttgaa gttcgtgaag cggtgcagtt cctgacgggt gagtatcgta acccgcgtct
    10021 gtttgatgtc acaatggcgc tgtgcgtaga gatgcttatc tccggcaaac tggcgaaaga
    10081 tgacgccgaa gcgcgcgcga aattgcaggc ggtgctggac aacggtaaag cggcagaagt
    10141 ctttggtcgt atggtagcgg cacaaaaagg cccgactgac ttcgttgaga actacgcgaa
    10201 gtatctaccg acagcgatgc tgacgaaagc agtctatgct gataccgaag gttttgtcag
    10261 tgaaatggat acccgtgcgc tggggatggc agtggttgca atgggcggcg gacgccgtca
    10321 ggcatctgac accatcgatt acagcgtcgg ctttactgat atggcgcgtc tgggcgacca
    10381 ggtagacggt cagcgtccgc tggcggttat ccacgcgaaa gacgaaaaca gttggcagga
    10441 agcggcgaaa gcggtgaaag cggcaattaa acttgccgat aaagcaccgg aaagcacacc
    10501 aactgtctat cgtcgtatca gtgaataacg gtatactgat ctgatcattt aaatttgaag
    10561 cactgagtac ggagaacata tgaaacgtgc atttattatg gtgctggact cattcggcat
    10621 cggcgctaca gaagatgcag aacgctttgg tgacgtcggg gctgacaccc tgggtcatat
    10681 cgcagaagct tgtgccaaag gcgaagctga taacggtcgt aaaggcccgc tcaatctgcc
    10741 aaatctgacc cgtctggggc tggcgaaagc acacgaaggt tctaccggtt tcattccggc
    10801 gggaatggac ggcaacgctg aagttatcgg cgcgtacgca tgggcgcacg aaatgtcgtc
    10861 cggtaaagac accccgtctg gtcactggga aatcgccggt gtcccggttc tgtttgagtg
    10921 gggatatttc tccgatcacg aaaacagctt cccgcaagag ctactggata aactggtcga
    10981 acgcgctaat ctgccgggtt acctcggtaa ctgccactct tccggtacgg tcatcctgga
    11041 tcagctgggc gaagagcaca tgaaaaccgg caagccgatt ttctatacct ccgctgactc
    11101 cgtgttccag attgcctgcc atgaagaaac tttcggtctg gacaaactct acgaactgtg
    11161 cgaaatcgct cgtgaagagc tgaccaacgg cggctacaac atcggtcgtg ttatcgctcg
    11221 tccgtttatc ggcgacaaag ccggtaactt ccagcgtacc ggtaaccgtc acgatctggc
    11281 tgttgaaccg ccagcaccga ccgtgctgca gaaactggtt gatgaaaaac acggccaggt
    11341 ggtttctgtc ggtaaaattg cggacatcta cgctaactgc ggcatcacca agaaagtgaa
    11401 agcgactggc ctggacgcgc tgtttgacgc caccatcaaa gagatgaaag aagcgggtga
    11461 taacactatc gtcttcacca acttcgttga cttcgactct tcctggggcc accgtcgcga
    11521 cgttgccggt tatgctgcgg gtctggagct gttcgaccgt cgtctgccgg agctgatgtc
    11581 tctgctgcgc gatgacgaca tcctgatcct caccgctgac cacggttgtg atccgacctg
    11641 gaccggtact gaccacacgc gtgaacacat tccggtactg gtatacggcc cgaaagtaaa
    11701 accgggctca ctgggtcacc gtgaaacctt cgcggatatc ggccagacgc tggcaaaata
    11761 ttttggtact tctgatatgg aatatggcaa agccatgttc tgatggattt gggcggagcg
    11821 ttgactccgc ctttgttatg tcacaaaaag gataaaacaa tggctacccc acacattaat
    11881 gcagaaatgg gcgatttcgc tgacgtagtt ttgatgcctg gcgacccgct gcgtgcgaag
    11941 tatattgctg aaactttcct tgaagatgcc cgtgaagtga acaacgttcg cggtatgctg
    12001 ggcttcaccg gtacttacaa aggccgcaaa atttccgtaa tgggtcacgg tatgggtatc
    12061 ccgtcctgct ccatctacac caaagaactg atcaccgatt tcggcgtgaa gaaaattatc
    12121 cgcgtgggtt cctgtggcgc tgttctgccg cacgtaaaac tgcgcgacgt cgttatcggt
    12181 atgggtgcct gcaccgattc caaagttaac cgcatccgtt ttaaagacca tgactttgcc
    12241 gctatcgctg acttcgacat ggtgcgtaac gcggtagatg cagctaaagc actgggcatt
    12301 gatgctcgcg ttggtaacct gttctccgct gacctgtttt actctccgga cggcgaaatg
    12361 ttcgacgtga tggaaaaata cggcatcctc ggcgtggaaa tggaagcggc tggtatctac
    12421 ggcgtcgctg cagaatttgg cgcgaaagcc ctgaccatct gcaccgtgtc tgaccacatc
    12481 cgcactcacg agcagaccac tgccgctgag cgtcagacca ccttcaacga catgatcaaa
    12541 atcgcactgg aatccgttct gttgggcgat aaagagtaat tgtgtttcgc tgaaaggcga
    12601 ttgccttgtg aagccggagc gggagactgc tccggcttt
//
LOCUS       DAZFYC010000140        12565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFYC010000140 DAZFYC010000000
VERSION     DAZFYC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.140"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            162..5586
                     /locus_tag="ACHKW5_004353"
                     /pseudo
     CDS             162..5586
                     /locus_tag="ACHKW5_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5795..6061
                     /gene="hipB"
                     /locus_tag="ACHKW5_004354"
     CDS             5795..6061
                     /gene="hipB"
                     /locus_tag="ACHKW5_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO7513605.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6061..7383
                     /gene="hipA"
                     /locus_tag="ACHKW5_004355"
     CDS             6061..7383
                     /gene="hipA"
                     /locus_tag="ACHKW5_004355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO7513606.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7695..7903
                     /locus_tag="ACHKW5_004356"
                     /pseudo
     CDS             7695..7903
                     /locus_tag="ACHKW5_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8335..8439
                     /locus_tag="ACHKW5_004357"
                     /pseudo
     CDS             8335..8439
                     /locus_tag="ACHKW5_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8602..9312
                     /locus_tag="ACHKW5_004358"
     CDS             8602..9312
                     /locus_tag="ACHKW5_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7513607.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9354..12005
                     /locus_tag="ACHKW5_004359"
     CDS             9354..12005
                     /locus_tag="ACHKW5_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7513608.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPELWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSNSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            12019..12549
                     /locus_tag="ACHKW5_004360"
     CDS             12019..12549
                     /locus_tag="ACHKW5_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7513609.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
BASE COUNT         3501 a         2700 c         3183 g         3181 t
ORIGIN      
        1 taatctatca attcgaaagt attagcaaat tagccaaatt gacaccttcc cacctccaac
       61 taacacttaa tacagacctc tgacaattta ataagtttca tttttaatgt ctgacgactc
      121 atctctttcg tggctgtatt gtttgtatat ggagcgttta tatgaataga atctatcgcg
      181 tgatatggaa ttgcactcta caggtatttc aggcctgctc ggaattaact cgcagggcag
      241 gtaaaacatc gacggttaat ttgcgtaaat cctctggact gacaacgaaa ttcagtagat
      301 tgacgctggg tgttttgctg gcactaagcg gttcagcgtc tggtgcaagt ctggaagttg
      361 ataatgatca gattaccaat attgatactg atgttgctta tgatgcctac ctggttggct
      421 ggtatggcac tggagtgctt aatattttgg ctggcggtaa tgcctcctta accactatta
      481 ctaccagcgt cattggcgct aatgaggact cagagggcac cgttaatgtt ttgggtggca
      541 cctggcgatt gtatgatagc ggaaataatg caaggccttt aaatgtgggt caatccggaa
      601 cggggacgct gaatattaaa cagaagggtc acgtcgatgg aggctattta agattaggtt
      661 cttcgacagg aggcgtcggg acggtcaatg ttgagggaga ggactctgtt ctgacgaccg
      721 aattattcga aatagggagt tacggtacgg gctcattaaa tattacggat aagggttacg
      781 tcacgagttc aatcgtcgcc attttaggct atcaggcggg cagtaatggc caggttgtcg
      841 ttgaaaaggg tggcgagtgg ctaataaaaa ataacgattc ctcaattgaa tttcaaattg
      901 gtaatcaagg aactggggag gcgactattc gcgagggtgg actgattacg gctgaaaata
      961 cgattatcgg tggcaatgcc accggtgtcg gaaccctgaa tgtgcaggat caagactctg
     1021 tcatcacggt acgcagactc tataatggat atttcggtaa tggcacactc aatatttcca
     1081 ataatggact aattaataac aaagaatatt cattagtggg cgttcaggac ggttcccacg
     1141 gtgtcgtcaa cgtgaccgat aaagggcatt ggaatttcct cggaacgggc gaagctttcc
     1201 gctatatcta tatcggtgat gctggcgacg gtgaacttaa tgtctcgcgt gaaggtaaag
     1261 tagattcggg aattatcact gcggggatga aagaaacagg cacaggcaac attactgtta
     1321 aggataagaa ctccgttatc actaatctcg gaactaatct tggttatgac ggccacggcg
     1381 aaatgaatat cagtaatgaa gggcttgttg tcagcaacgg aggaagctca ctcggttatg
     1441 gagaaaccgg cgtcgggaat gtcagcatca ccacgggggg gatgtgggag gtcaataaga
     1501 atgtctatac caccattggt gttgcgggcg tcggaaacct caatattagc gatggcggta
     1561 agttcgtatc gcaaaatatt acttttttgg gcgataaagc aagcggtatc ggcacactga
     1621 acctgatgga tgcgacatca tcgttcgata ctgtgggtat caatgtcggt aattttggta
     1681 gcggtatcgt aaatgtcagt aatggtgcca cccttaattc aacgggctat ggatttatcg
     1741 gaggaaatgc ctccggtaag gggatagtta atatttcaac ggacagtctc tggaatttaa
     1801 agacatcatc tactaacgca caattgctac aggtcggtgt attaggcaag ggtgaactga
     1861 atattaccac cggaggtata gttaaagcgc gtgatacaca gatagctctc aatgacaaaa
     1921 gtaagggcga cgtgagggtg gatggatggg cagaactctc ttcttgaaac attcaatatg
     1981 tacgtaggga catctggtac gggtacgtta accctgacga atagcggcac gctgaatgtc
     2041 gaaggtggag aagtttactt aggtgttttt gaacctgctg taggaacgct aaacattggt
     2101 gcagctcacg gtgaggcggc ggcagatgcc gggtatataa ccaacgcgac gaaagtagag
     2161 tttggttctg gcgaaggtgt ttttgtcttt aatcatacta ataacagtga tgccggctac
     2221 caggtcgata tgctgattac aggtgacgat aaagacggaa aagtgatcca tgatgcaggc
     2281 catacggtgt tcaatgcagg gaatacttat agcggtaaaa cgctggtcaa tgacggcctc
     2341 ctgaccattg cgtctcatac ggcagatggg gtaacgggca tgggttcgag tgaagtcacc
     2401 attgcaagcc ccggtacgct cgacattctc gcatcaacga acagtgcagg agattacacg
     2461 ctgaccaatg cgctcaaagg cgatggcttg atgcgagtgc agctgtcatc ctacgacaag
     2521 atgtttggct ttacccatgc aacagggact gaattcgccg gtgttgccca actgaaagac
     2581 agtaccttca ctctggaacg cgacaacacc gctgcgctta ctcacgcgat gttgcagtct
     2641 gacagtgaaa ataccacatc ggtaaaagtg ggagagcaat ccattggtgg actggccatg
     2701 aatggcggta cgctcatttt cgatacggat attcctgctg cgacgcttgc agagggatat
     2761 atcagcgtcg atacgctggt tgtcggcgcg ggtgactaca cctggaaagg ccgtaactat
     2821 caggtaaacg ggacgggcga cgtgcttatc gacgtgccta aaccgtggaa tgatcccatg
     2881 gcgaataacc ctctgacgac gctcaatttg ctggaacacg acgatagcca tgtcggcgtt
     2941 caactggtga aggcgcaaac ggttattggg tcgggtggct cattaacgtt acgtgattta
     3001 cagggcgacg aggtggaagc ggacaaaacg ttacacattg cgcaaaacgg aacggtggtc
     3061 gccgagggtg attatggatt ccgcctcacg accgcaccag gtgatggttt gtacgttaac
     3121 tatgggctga aagcgctgaa catccatggt gggcaaaagc tgacgttagc cgaacatggc
     3181 ggagcctatg gcgcaacggc cgatatgtcg gcaaaaatcg gtggtgaagg ggatctggca
     3241 atcaatacgg tgcgacaggt ttcgctttcc aacggtcaga acgactatca gggggcaacc
     3301 tacgttcaga tggggacatt acgtaccgat gcggatggtg cacttggcaa cacccgggaa
     3361 ctgaacatca gcaacgcggc catcgtcgat cttaatggat cgacgcagac ggtagagaca
     3421 ttcaccgggc agatgggttc gactgttttg ttcaaagagg gggcgctgac ggtaaataaa
     3481 ggtgggatca gtcagggtga actgacaggt ggcggaaacc tgaatgttac agggggaacg
     3541 ctggctatcg aggggcttaa tgcacgctac aatgcgttaa ccagcattag cccaaatgcg
     3601 gaagtcagcc tcgataatac tcaggggtta ggcagaggaa atattgccaa tgacggtctg
     3661 ttaacgctaa aaaacgtgac tggcgaactg cgtaatagca taagcgggaa gggtatcgtg
     3721 agcgcaaccg ccaggacaga tgtagagctg gatggcgata atagccgctt tgtggggcaa
     3781 ttcaacattg atacaggcag cgcgctcagc gtcaacgagc agaaaaacct gggtgatgct
     3841 tccgttatca ataatggcct gctcaccatc tccactgagc gtagctgggc gatgacgcac
     3901 agtatcagcg gtagcggtga tgtgacaaaa ctgggtaccg ggatcctgac tcttaacaac
     3961 gattccgcgg cgtatcaggg tacgacggat atcgtggggg gggaaattgc tttcggttcc
     4021 gactctgcca ttaatatggc aagtcaacac attaatatcc ataacagcgg tgtgatgtcg
     4081 ggaaatgtca ccactgcagg tgatgtgaac gttatgcctg gggggacact gcgtgtcgct
     4141 aaaaccacaa tcggcggcaa cctggagaat ggcggcacgg ttcaaatgaa tagcgaaggg
     4201 gggaaaccgg ggaatgtact gaccgttaac ggcaactata ccggaaacaa tggcctgatg
     4261 acgttcaacg cgacgctggg cggcgataat tcgcccaccg ataaaatgaa cgtgaaaggc
     4321 gatacccaag ggaacactcg cgttcgggtt gataacattg gcggcgtcgg tgcacaaacg
     4381 gtcaacggta ttgaactcat tgaggttggc ggtaattctg caggtaactt cgcgctgacc
     4441 accggaactg tcgaagctgg ggcttacgtc tacacgctgg ctaaagggaa ggggaatgac
     4501 gagaaaaact ggtatctgac cagtaaatgg gacggcgtaa cgccaccgga tacgcccgac
     4561 cccatcaata atccccctgt tgtggatccg gaaggcccat cagtttatcg cccggaggcc
     4621 ggaagctata tcagcaacat tgccgcagcc aactcgctgt ttagccatcg tttacactac
     4681 cgtctgggtg agccgcagta tatagattca ctgcattctc aggggtcggc aagcagtatg
     4741 tggatgcgtc atgtcggagg gcacgaacgt tcaagggccg gtgacggtca gctaaatact
     4801 caggctaacc gctatgtatt gcagctaggc ggcgatttgg cgcagtggag tagcaacgcg
     4861 caggatcgct ggcatcttgg cgtgatggca ggttacgcta atcagcacag taatactcag
     4921 agtaatcgtg tgggttataa atcggatggg cgcatcagcg gttacagcgc tgggctgtac
     4981 gcgacctggt atcagaacga tgcgaataag accggcgctt atgttgacag ctgggcgctg
     5041 tataactggt ttgataacag cgtcagttcc gataaccgtt ctgctgacga ctatgattct
     5101 cgcggtgtga cggcctctgt tgagggtggg tatacctttg aagcgggaac atttagcggc
     5161 agcgaagaga cgctgaatac ctggtacgtc cagccacagg cgcaaatcac ctggatgggt
     5221 gtgaaagatt ccgaccatac ccggaaagac ggaacgcgca ttgaaacgga aggcgacgga
     5281 aatgtgcaaa cgcgacttgg ggtgaaaacc tacctgaaca gccatcacca gcgtgacgat
     5341 ggtaaacagc gtgagttcca gccttacatt gaagcgaatt ggatcaacaa cagcaaagtc
     5401 tacgccgtga agatgaatgg tcaaaccgta ggccgtgaag gtgcgcgtaa tctcggtgaa
     5461 gtacgtaccg gggttgaggc gaaagtaaat aacaacctta gcctgtgggg gaatgtcggt
     5521 gtgcaactag gtgataaagg ctatagcgat actcagggta tgctgggagt gaaatatagc
     5581 tggtaaaccg tataagccgc atgtcgagat ggcatgcggc ttaatattgc cgacttcaaa
     5641 ggctttaaag cctccctttt atccgcgatc gcggatatcg cagcgtttat cccgtacagc
     5701 ggataagatg tgtttccaga ttgactttat cctcactaaa ggataaaact gataatatcc
     5761 ccttaagcgg ataaatttgc tgtggacgta tgacatgatg agctttcaga agatctatag
     5821 cccaacgcaa ttggcgaatg caatgaaact ggttcgccag caaaatggct ggacgcagag
     5881 cgagctggcg aaaaaaattg gcattaagca ggcgacaatt tccaatttcg aaaacaaccc
     5941 tgacaatacc acgctcacga cattttttaa gattttacag tcgcttgaac tctcaatgac
     6001 actatgcgac acgaaaaatg cctcgccaga atcaacagaa cagcaagatc tggagtggta
     6061 atgcctaaac ttgtcacttg gatgaacaac cagcgggtag gcgagttaac gaagttagcc
     6121 aacggcgcgc acacctttaa gtatgcaccg gagtggttag ccagccgtta tgccagaccg
     6181 ttgtcacttt cgctgccatt gcagaggggg aatatcacct cagatgccgt atttaacttc
     6241 ttcgataacc tgttacccga tagcccgatt gtacgtgacc ggatcgttaa acgttatcat
     6301 gctaaatcca gacaaccgtt tgatttattg tcagaaatag ggcgagacag cgttggtgcc
     6361 gtaacgttat tacccgaaaa tgaaaccata acgcgcccga taatggcatg ggaaaagctt
     6421 actgaagcca ggcttgaaga agtattaacg gcttataaag cagatatccc tctgggcatg
     6481 attagagaag aaaatgactt tcgcatctcg gttgctggcg cgcaggagaa gacagcgctg
     6541 cttagaatag gcaatgactg gtgcattccg aaaggaataa cgccgacgac gcacatcatt
     6601 aaattaccga ttggcgaaat caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt
     6661 gataatgagt attactgtct gctgctggcg aaagaacttg ggttgaatgt tccggacgca
     6721 gaaatcatta aagcgggaag ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg
     6781 aatactgagc gaacggtttt acttcgcttg ccacaggagg atatgtgtca gacattcggt
     6841 ttaccttcat ctgtgaaata tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg
     6901 tttttgatgg ggtccagcga ggcgcttcgc gatcgttatg attttatgaa attccaggtc
     6961 ttccagtggt tgattggcgc aacggatggc catgcaaaaa acttctccgt atttattcag
     7021 gctggcggca gttatcgact cacgccattt tacgacatca tttcagcatt tccggtcctt
     7081 ggcggtacgg gaatacacat cagcgatctc aaactggcaa tggggcttaa cgcatccaaa
     7141 ggcaaaaaaa cggcaatcga taaaatttat ccgcgacatt ttttggcgac agcaaaggtg
     7201 ctgagattcc cggaagtgca gatgcatgaa atcctaagtg actttgccag aatgattccg
     7261 gcagcactgg ataacgtgaa gacttcatta ccgacagatt ttccggagaa cgtggtgacg
     7321 gcagttgaaa ccaatgtgtt gaggttgcat ggacggttaa gccgagaata cggtagtaag
     7381 tgagatatgg ggtttggtcg ttgttaatga gcacgacaat catgaccgcc aacgactgga
     7441 ttcttccttg gtttatactg gtgcgcataa tgtatattat gttaaatcat gtctgagtga
     7501 tgcgaagtgc ttttctggta gtcgttattc gttcagggtt caagaactta agcctctcct
     7561 ctttcataat tggcctgtgc atggagaaat agcctggtta ttccctgccc catgctaaga
     7621 gcgcagtgtc tgtcgcatag acactgcata cgaaaacacc agagggtgct gttacttatt
     7681 taaggacgga tcaagcggga atgagtccac tccggtatca gaaatggcta cgtctcaatg
     7741 aagtcaggcg accgatgctg aatgaacatt acgatgtcac cacagcagcc tatgctgtcg
     7801 gttacgaaag ctatcccatt tcagtcggga atattcgcgg atgtttggag agtcacccaa
     7861 gagagatatt accaggttaa gaaagtctgt cggtcagctt taataaaccc tgcacttatc
     7921 tgtttagagc aattcggtgt tagtttcagc aagcaaacat taaccatagc taatgattta
     7981 tagccatatt aaccattggt agcccttgac ctttcctgac acttcccttg ttcgcaataa
     8041 tattctgttt agtcacggtt tataaataac atgcataatg aaatcgtcat gttatttagc
     8101 gacataaaat gtttttgcaa atactctctt caacaagtgc acaataaact tgttgtgatt
     8161 aggtgtcctt gtgatttaaa gatattatcg ttcttcgtga aatgtaataa ccctggaaac
     8221 tatattctca gggcaacaac caatcaagaa tgattcttgt ttttaagcaa taacagaatg
     8281 acttaattgt taaccctctt tattcagcta cttaaatata aattttggag aattatgaaa
     8341 ctcaaacatg ttggtatgat tgtcgtttct gtgttggcga tgtcgtctgc tgcggtaagc
     8401 gcagccgagg gtgatgaatc agtaacgacc actgttaatt tactttgtag caaggttttc
     8461 ctattaaaag agaaccaaca tttattacaa acgcattaag tctggaaacc cggcgactcg
     8521 ccaatgtatt agcaacatta atcgattgtc tgatgtaacg gttttcccgc ttatcctctt
     8581 aacacatgga gaatgcatca gatgcaaact acgcgtaccc cctactctat ttcctttatg
     8641 gcgacagtgt tacttttatt gctctttgct tgccatagca ccgttgccaa cgcagctgtc
     8701 gcactgggtg cgacgcgggt aatttatccg gcaaaccaga aacaggtttt attgcctgtc
     8761 acgaataatg atcctgccag tgtctatctc attcagtcat ggatagaaaa tgcaggtgac
     8821 caaaaagaca cccagtttgt cattacccca ccgctttttt ccatgcaagg taaaaaagag
     8881 aatacgctgc ggataatcaa cgctactaat catcagttac caggcgaccg tgaaagcctg
     8941 ttctgggtga atgttaaagc tattcctgct atggaaaaag accagaagaa tgagaataca
     9001 ttacagctgg caattattag ccgcataaaa atgttttatc gccccacgaa cctggcaatg
     9061 gcacctgaag aagcgccagc aatgcttcgg tttcgtcgca gcggtagcaa actcactctt
     9121 attaatccaa cgccctactt cattaccgta acaaatatga aagcaggaaa tagcaatctc
     9181 ccaaacacca tggtgccgcc aaaaggtgaa gtatcggtgg atatcccgca tgctgcaacc
     9241 ggggatatca gttttcagac gatcaatgat tacggtgcat taactccgcg tattaaggca
     9301 accatgcagt aagccagtga cttaattaat aataaactca atcggaatta ataatgacag
     9361 cctttcgtgc cgcatttaag gcatatcgta tgcatcaggt gttaatacta ccacgctttg
     9421 cacggcttac cattgccctt ggcttagcaa ctgcggtttt tcccgttgat gcagagtatt
     9481 attttaatcc acggttttta agcaatgatc ttgctgaatc tgtcgattta tccgccttta
     9541 ctaagggacg cgaggcacca cctggtacat atcgggtcga catttatttg aacgacgaat
     9601 ttatgactag tcgggatatt acttttattg cagatgataa taacgcagat ctcatcccat
     9661 gtctgagtac agaccttctt gtcagtcttg gcataaaaaa atctgcgtta ttagacaata
     9721 aggaacattc tgcagaaaaa catgtgccgg acaacagcgc atgcacacca cttcaggatc
     9781 gtttggccga cgcatcaact gagtttgatg ttggtcagca acatttgtca ctctccgttc
     9841 cgcaaattta tgttggcaga atggctcgcg gctatgtttc tcctgaactg tgggaagaag
     9901 gaataaatgc tgggcttcta aattatagtt ttaatggtaa ttccattaat aatcgtagta
     9961 accataatgc aggaaaatcc aactatgcat atttgaattt acagagtggc atcaacattg
    10021 gtagttggcg actgcgcgat aactcaacgt ggagttataa cagtgggagt agcaattcat
    10081 ctaacagcaa taaatggcag catatcaata cgtcggctga acgtgacatt attcccttac
    10141 gctcacggtt aacggtaggt gatagttata ccgatggcga tatttttgat agtgtgaact
    10201 ttcgtggcct caaaataaat tcaacagaag cgatgttgcc cgatagccaa catggtttcg
    10261 ctccggtgat tcatggtatt gcccgtggca ccgcacaagt gagtgtaaaa cagaacggat
    10321 acgatgttta tcagactact gttccacccg gcccttttac tattgatgat atcaactctg
    10381 ccgccaatgg tggtgatctg caagtaacca taaaagaggc agacggcagt attcagacat
    10441 tatatgttcc ttattcgtct gttccggttc tccaacgtgc tggatatacg cgttatgcgc
    10501 ttgccatggg ggaatatcgt agtggaaata acctgcaaag ctcccccaag ttcatacaag
    10561 gtagcttgat gcatggactg gaaggaaact ggacacctta tggcggaatg caaattgcag
    10621 aagattatca ggccttcaac cttggtattg gtaaagattt aggacttttt ggtgcctttt
    10681 ctttcgatat cacgcaggcc aatacgacac ttgcagatgg cacccgtcac agcgggcaat
    10741 cggttaaatc cgtctacagc aaatccttct accagacagg aaccaatatc caggtcgcag
    10801 gatatcgcta ttctacgcaa ggtttttata acttatccga cagtgcctac agtcgaatga
    10861 gtggttacac cgtcaagcct cctaccggag acaccaatga gcagacacaa tttattgatt
    10921 attttaatct gttctacagt aagcgtggtc aggaacaaat aagcatctct cagcagcttg
    10981 gaaattacgg tacgacattt ttcagtgcca gtcgccaaag ttactggaac acgtcacgca
    11041 gcgaccagca aatatcattt ggattaaatg tgccgtttgg tgatattacg acttcgctga
    11101 attacagcta ttccaataat atatggcaaa acgatcggga tcatttactc gcttttacgc
    11161 ttaatgttcc cttcagtcat tggatgcgta cagacagtca gtcggcattt cgtaattcaa
    11221 acgccagtta cagtatgtca aacgatttga aaggcggcat gaccaatcta tcgggggttt
    11281 atggcactct gctgccggat aataacctga attatagcgt tcaggtcggt aacacccacg
    11341 gaggtaatac atcgtctggc accagtggtt acagttctct taattatcgt ggagcttacg
    11401 gtaatacgaa tgtcggttac agtcggaatg gtgacagcag ccagatttat tacggaatga
    11461 gtggtgggat tattgctcat gctgatggca tcacctttgg acagccgctg ggcgacacaa
    11521 tggttctggt taaggctcct ggcgctgata atgtcaaaat agagaaccag accggaattc
    11581 ataccgactg gcgtggctat gccatattac catttgcgac agaatataga gaaaaccgtg
    11641 ttgctcttaa cgcgaattcc cttgcagata atgttgaact ggatgaaacc gtggtcactg
    11701 tcatcccaac tcacggtgct attgccagag caacatttaa tgcacaaatc ggcgggaaag
    11761 tattaatgac gttgaagtac ggtaataaga gcgttccatt cggtgcaatt gtcacacacg
    11821 gagagaataa aaatggcagc attgtcgcag aaaacggtca ggtttatctg actggacttc
    11881 cacagtcagg gaaattacag gtttcatggg gcaaagataa aaactcaaac tgtattgtcg
    11941 agtacaagct tcctgaagtt tctcctggta ccttactgaa ccagcagaca gcaatctgtc
    12001 gctaaggaac aatagattat gaaatacaat aacattattt tcctcggttt atgtctgggg
    12061 ttaaccacct attctgcttt atccgcagat agcgttatta aaattagcgg gcgcgtcctc
    12121 gattatggct gcacagtctc atcggattcg cttaatttta ccgtagatct ccaaaaaaac
    12181 agtgccagac aatttccaac gaccggtagc acaagtccag tcgtcccttt tcagattacg
    12241 ttaagtgaat gcagcaaagg gacaacgggg gttcgggttg catttaacgg tattgaggat
    12301 gcagaaaata atactttgtt gaaactggat gaaggaagca atacggcctc cggtttgggt
    12361 atagaaatat tggacgcaaa tatgcgtccg gtgaaactga atgaccttca tgccgggatg
    12421 cagtggatcc cactggtacc agaacagaac aatattttgc cttactccgc tcgtctgaag
    12481 tcaactcaga agtccgtcaa tccgggactg gtgagggctt cggcaacctt tacccttgaa
    12541 tttcaataat cagagtcaga tatga
//
LOCUS       DAZFYC010000141        12520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFYC010000141 DAZFYC010000000
VERSION     DAZFYC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.141"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..747
                     /locus_tag="ACHKW5_004361"
     CDS             <1..747
                     /locus_tag="ACHKW5_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO7513610.1"
                     /translation="AFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAM
                     GCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGR
                     EEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADL
                     TAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDK
                     VFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1016..3253
                     /gene="nrfB"
                     /locus_tag="ACHKW5_004362"
     CDS             1016..3253
                     /gene="nrfB"
                     /locus_tag="ACHKW5_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO7513611.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3240..6212
                     /gene="nfrA"
                     /locus_tag="ACHKW5_004363"
     CDS             3240..6212
                     /gene="nfrA"
                     /locus_tag="ACHKW5_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO7513612.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTSEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6213..7103
                     /gene="ybcH"
                     /locus_tag="ACHKW5_004364"
     CDS             6213..7103
                     /gene="ybcH"
                     /locus_tag="ACHKW5_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO7513613.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7286..8047
                     /gene="envY"
                     /locus_tag="ACHKW5_004365"
     CDS             7286..8047
                     /gene="envY"
                     /locus_tag="ACHKW5_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO7513614.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8560..9513
                     /gene="ompT"
                     /locus_tag="ACHKW5_004366"
     CDS             8560..9513
                     /gene="ompT"
                     /locus_tag="ACHKW5_004366"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO7513615.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9700..11184
                     /locus_tag="ACHKW5_004367"
     CDS             9700..11184
                     /locus_tag="ACHKW5_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7513616.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHYLITPQKIENIYCPGNSEAEIQ"
     gene            complement(11368..11580)
                     /locus_tag="ACHKW5_004368"
                     /pseudo
     CDS             complement(11368..11580)
                     /locus_tag="ACHKW5_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            11730..12398
                     /locus_tag="ACHKW5_004369"
     CDS             11730..12398
                     /locus_tag="ACHKW5_004369"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7513617.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT         3459 a         3029 c         2948 g         3084 t
ORIGIN      
        1 gcgttctcaa caatgcacag tctggtcatc ggacagatca agacggatga gaaatctaat
       61 gagattacag ctatcccaga acttcttaac atgctggata ttaaaggaaa aatcatcaca
      121 actgatgcga tgggttgcca gaaagatatt gcagagaaga tacaaaaaca gggaggtgat
      181 tatttattcg cggtaaaagg aaaccagggg cggctaaata aagcctttga ggaaaaattt
      241 ccactgaaag aattaaataa tccagagcat gacagttacg caatcagtga aaagagtcac
      301 ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact tattgatttc
      361 acgtttgaat ggaaaggtct gaagaaatta tgcgtggcag tctcctttcg gtcaataata
      421 gcagaacaaa agaaagagcc cgaaatgacg gtcagatatt atatcagttc tgctgattta
      481 accgcagaga agttcgccac agcaatccga aaccactggc acgtggagaa taagctgcac
      541 tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa taagaagagg aaatgcagca
      601 gaattatttt cagggatacg gcacatcgct attaatattt tgacgaatga taaggtattc
      661 aaggcagggt taagacgtaa gatgcgaaaa gcagccatgg acagaaacta tctggcgtca
      721 gtccttgcgg ggagcgggct ttcgtaatct tgccctgcct gctcacctcc tccaggcact
      781 tccactacat gatgacctga tcggtgccac cactcctctt cactatgacg ttaacctccc
      841 ctccttgaca ccacgctttt gtctacattc tacgcaaacg gcaactttta taaccagtgt
      901 aaaaataacg tgccgcaata gtcataccta ttaatggtaa aaagctgtca caattcataa
      961 aaaaccttaa tatacgccac cctaaacata accagcgtta atgtaaggtt tttgtgtgga
     1021 ctggcttctt gatgtttttg caacctggct ctacggctta aaagtaatcg cgataacgtt
     1081 agcggtcatc atgttcatca gcggactgga cgattttttt attgatgtcg tctactgggt
     1141 acgccgcatt aaacgcaagt tgagtgttta tcgccgctac ccgcgaatga gttaccgcga
     1201 actgtataaa ccagatgaaa aaccgttagc gattatggtt ccggcgtgga atgaaacggg
     1261 cgtcatcggc aatatggccg agctggcggc gaccacgctc gactacgaaa actatcatat
     1321 ctttgttggc acctacccca acgaccccga tactcaacgt gatgttgacg aagtgtgcgc
     1381 tcgctttccg aacgtgcata aggtagtctg cgcgcgtcct ggtcccacca gcaaagccga
     1441 ctgtctgaac aacgtgctgg acgccatcac ccagtttgaa cgtagcgcca atttcgcttt
     1501 tgctggtttt attctgcatg acgccgaaga tgtgatttca ccgatggaat tgcgtctgtt
     1561 caactatctg gtcgagcgta aagatctgat tcagatcccg gtgtatccgt tcgaacgcga
     1621 atggacgcac ttcaccagca tgacttacat tgatgagttt tcagagctgc atggcaaaga
     1681 tgttccggtg cgtgaagccc tcgccggaca agtgcccagc gcaggcgtcg gcacctgttt
     1741 cagccgccgc gccgtgaccg ctctgttagc tgacggtgac ggtattgctt tcgacgtgca
     1801 gagtctgact gaagattacg acattggttt ccgcctgaaa gagaaaggta tgacggaaat
     1861 ttttgtccgt tttccggtgg tggacgaagc caaagaacgc gagcagcgta aatttttaca
     1921 gcacgcacgg acgtcaaaca tgatctgcgt gcgcgaatat ttccccgata ccttttcgac
     1981 tgcagttcga caaaaatctc gctggatcat cggcattgtt ttccagggct ttaaaaccca
     2041 caaatggacc tccagcctga cgctgaacta ctttctctgg cgcgaccgca aaggggcaat
     2101 cagtaacttt gtcagcttcc tcgcgatgct ggtgatgctc cagcttttgc tgttgctggc
     2161 gtatgaaagt ttgtggcccg atgcctggca tttcctttct atttttagcg gcagcgcatg
     2221 gttaatgacc ctgctgtggc taaactttgg cttgatggtt aaccgcatcg tgcagcgggt
     2281 gattttcgtc actggctact acggtctgac gcaggggctg ttatctgtcc tgcgtctttt
     2341 ctggggcaac ctgattaact tcatggccaa ctggcgcgcg ctaaaacagg tacttcaaca
     2401 cggcgatcca cgtcgcgtcg cgtgggataa aacaacgcat gacttcccca gcgtgactgg
     2461 cgatacccgc tcgttgcgcc cgttaggcca aattctgctg gaaaatcagg tcatcactga
     2521 agaacaactc gatacagcac tgcgtaatcg cgtcgaaggt ctacgcttgg gcggttcaat
     2581 gctgatgcag gggctgatta gcgccgagca gctggcacag gcgctggcag agcaaaacgg
     2641 cgtggcgtgg gaatccatcg atgcctggca gatcccgtcc tcgctgattg ccgaaatgcc
     2701 ggcctccgta gcgctgcatt atgcagtact gccgctgcgt ctggaaaatg atgagttaat
     2761 tgtcggcagt gaagatggta ttgacccggt ttcgctggcg gccctgacgc gtaaagtcgg
     2821 acgcaaagtg cgttacgtca ttgttctgcg gggacaaatt gtcacggggt tacgtcactg
     2881 gtatgcacgc cgacgcggtc acgatccgcg ggcaatgttg tacaatgcgg ttcagcatca
     2941 gtggctcacg gaacagcagg ccggtgaaat ctggcggcaa tatgtgccgc atcagttcct
     3001 gttcgccgaa atactgacca cgctcggtca tattaatcgt tcagcaatta acgtgttgtt
     3061 attgcgccat gaacgcagtt ctctgccgct cggcaagttt ttggtcaccg aaggcgttat
     3121 cagccaggaa acgttggatc gcgtcctgac aattcaacgc gaattacaag tttcgatgca
     3181 atcactatta ctcaaagcag gtttaaacac agaacaggtt gcgcaactgg agtccgaaaa
     3241 tgaaggagaa taaccttaat cgcgtcatcg gatggtctgg tttactgctg acgtctttat
     3301 tgagtaccag cgcactcgca gacaatatcg gcaccagcgc agaagagctg gggctgagcg
     3361 attatcgcca ttttgttatt tatccccgtc tcgataaggc gctgaaggca cagaaaaata
     3421 acgacgaagc aaccgccatc cgcgaatttg aatatataca ccagcaggtg ccggataata
     3481 ttccgctgac tttatacctt gcggaagcct atcgccattt tggtcatgat gaccgggcgc
     3541 ggctgttgct tgaggatcaa ctgaaacgtc acccaggaga tgcccgactt gagcgcagtc
     3601 tggcggctat tccggttgaa gtgaaaagcg ttacgactgt tgaagaactg cttgcccagc
     3661 aaaaagcgtg cgacgctgcg ccgaccctgc gttgtcgcag tgaagtcggg cagaatgccc
     3721 tgcggctggc acaattacct gtcgccagag cgcaactgaa cgatgcgacg tttgctgcat
     3781 cgccagaagg aaaaacgctg cgaaccgatc tgctgcaacg ggcaatctac ctgaaacaat
     3841 ggtcccaggc agatacgcta tacaatgaag cacgccagca gaacacatta agcgcggcag
     3901 aacgccgtca gtggtttgac gtgcttcttg ccgggcagct ggacgatcgg atcctggcac
     3961 tgcaatcaca ggggatcttc accgatcctc agtcatatat tacttacgcg accgcgctgg
     4021 cttatcgtgg cgaaaaagca cgcctccagc attatctcaa tgaaaataag ccgctgttta
     4081 ccacggacgc acaagagaaa agttggctct atctgttatc taaatacagc gccaaccccg
     4141 ttcaggcatt ggcgaattat acggtgcagt ttgccgataa ccgccagtat gttgttggcg
     4201 cgacgctacc ggtgctgtta aaagaaggtc agtacgacgc agcgcaaaaa ctgctcgcca
     4261 ccctccccgc caatgaaatg cttgaggagc gttatgctgt cagcgtggcg acccgtaaca
     4321 aggctgaatc tctgcgtctg gcacgattgc tgtatcagca agaaccggca aatcttaccc
     4381 gcctggatca actgacctgg caactgatgc agaacgagca gtcacgcgaa gctgccgatt
     4441 tattgctgca acgctatcct ttccagggcg atgcgcgtgt cagccagact ttaatggcgc
     4501 gactggcgtc tctgctggaa agtcatcctt acctggcaac gccggcgaag gtggcgattt
     4561 tatcgaaacc cttaccgctg gcggagcaac gtcagtggca aagtcagttg ccgggtattg
     4621 cagataattg cccggcaata gttcgcttgc tgggcgatat gtcgccttcc tacgatgccg
     4681 ccgcctggaa ccgtctggca aagtgttatc gggacacgct acccggtgtg gcgctgtatg
     4741 catggcttca ggccgaacaa cgacaaccga acgcctggca acatcgtgcg gtagcctatc
     4801 aggcgtatca ggttgaggac tacgccaccg cactggcggc ctggcagaaa atcagtcttc
     4861 acgacatgag caatgaggat ctgcttgctg ctgccaatac cgcccaggcg gcaggaaatg
     4921 gtgcagctcg cgatcgctgg ctacaacagg cagaacaacg tggactggga aacaatgccc
     4981 tctactggtg gctgcatgcg caacgttaca ttcctggtca gccggaactc gcactgagcg
     5041 atctcacgcg ctcaatcaat attgcccctt ctgccaacgc ttacgttgcg cgggcgacaa
     5101 tttatcgcca acgtcataat gtcccggcgg cggtgagtga tttgcgcgcc gcgctggaac
     5161 tggaaccgaa taatagcaac atccaggcag cgcttggtta cgccttgtgg gatagcggtg
     5221 atatcgcaca gtcgcgggaa atgctcgaac aggcgcataa agggcttccg gacgatccgg
     5281 cactgatccg acaactggcc tacgtgaacc agcgtctgga tgacatgcct gcgacgcagc
     5341 actacgcccg gctggtgatt gatgacattg ataatcaggc gctgataacc ccactgacct
     5401 cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca tgaggaggtc ggtcgccgct
     5461 ggacgttcag tttcgattct tctatcggct tgcgttccgg ggcaatgagc accgctaaca
     5521 ataatgtcgg cggcgcagcg ccagggaaaa gctatcgtag ctacgggcaa ctggaagccg
     5581 agtaccgcat cggacgcaat atgctgctgg aaggcgacct gctctcagtt tatagccgcg
     5641 tctttgccga taccggagaa aacggggtga tgatgccggt gaaaaatccg atgtccggca
     5701 ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc gaacagcagt
     5761 tgccgctgaa cggccaaaat ggcgcatccg ataccatgct gcgcgccagc gcctcattct
     5821 ttaatggcgg caaatacagc gacgaatggc acccgaacgg ttcaggctgg tttgcccaaa
     5881 acctgtacct cgatgcggcg caatatatcc gccaggatat tcaggcgtgg acggcagatt
     5941 atcgcgtcag ctggcatcag aaggtagcta acggacagac tattgagcct tacgctcacg
     6001 ttcaggacaa cggctatcgt gataaaggca ctcagggcgc gcagcttggc ggagtcgggg
     6061 tccgctggaa tatctggacc ggcgagacgc actacgacgc ctggccgcac aaagtcagtc
     6121 tcggcgtcga gtatcaacat acctttaagg cgattaatca acgtaacgga gagcgcaaca
     6181 acgcgtttct caccattgga gtgcactggt aaatgcgtaa gttcattttc gtattgctga
     6241 cactgctttt ggtcagccct ttttcctttg cgatgaaagg tattatctgg caaccacaaa
     6301 accgagatag tcaggttacc gatacccagt ggcaggggct gatgagtcag ttacgtttgc
     6361 aaggcttcga taccctggtt ttgcaatgga cccgttacgg cgatgcattc acccagccag
     6421 aacagcgcgc gttattgttt aagcgtgccg cagctgcgca acaggctggc ctgaagctga
     6481 ttgtcgggct gaacgccgat ccggaatttt ttatgcacca gaaacagtcg tccgcagcgc
     6541 tggaaagcta tcttaatcgc ctgctggctg ccgatctcca gcaagccaga ttatggagcg
     6601 ccgcgcctgg cgtaacgccg gatggctggt acatcagcgc ggaaattgac gacctgaact
     6661 ggcgcagcga agctgcccgt cagcctttgc taacatggtt aaacaacgcg cagcggctga
     6721 ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt tttcgccgga aacatgtcgc
     6781 ccgatggcta tcgccaactg ctggaacaag ttaaagcaac cggcgttaat gtctgggtac
     6841 aggatggcag cggcgtggat aaacttaccg ctgaacagcg tgaacgttat ctacaggcca
     6901 gcgccgattg ccaaagtccc gcccctgcca gcggcattgt ttatgaactg tttgtcgccg
     6961 gcaaaggcaa aacctttaca gcgaaaccga aaccggatgc agagatcgcc tcgctgttag
     7021 cgaaacgttc ctcttgcggt aaagacactc tctatttctc tctgcgctat ttgcccgtcg
     7081 cgcacggcat tctcgagtat taaatctcct ctaggtaagt cgggtacgac ctggcttacc
     7141 tctttcgctc tcgtcagaat tccatcaaga ttaattgcta aaaagcgtgc taaagaaaga
     7201 agatcgttat cggcgaacgg gccgatcact catgcaggaa gcatagatcc tttgagcgga
     7261 gtttctgata tctgaggagt gcgaaatgca attgagcagc agtgaacctt gcgtggtgat
     7321 cctgaccgaa aaagaggtag aggtaagcgt caataaccat gctacgttta cccttccgaa
     7381 aaactacctg gccgccttcg cgtgcaacaa taacgtcatt gaactctcaa cgttaaatca
     7441 cgtattaatc acccacatca accgtaacat catcaacgat tatctgttgt ttttaaataa
     7501 gaacttaacc tgtgtaaagc cctggtcgcg gctggcaacc ccggttattg cttgtcatag
     7561 ccgtacaccg gaagtgttcc ggctagccgc caaccacagc aagcagcaac ccagcaaacc
     7621 ctgcgaagcg gagttgacgc gcgtattgct ttttaccgta ttgtctaact ttcttgagca
     7681 atcgcggttt attgccctac tgatgtatat cttacgcagc agcgtccgcg acagcgtctg
     7741 ccgcattatt caaagcgata ttcagcatta ctggaatctg cgaattgtcg ccagttcgct
     7801 atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac gaaaatacca gctatagcca
     7861 gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg ttattgatgg ataacaaaaa
     7921 tatcactcag gtggcgcaat tatgtggcta tagcagcacg tcatacttta tctctgtttt
     7981 taaggcgttt tacggcctga caccgttgaa ttatctcgcc aaacagcgac aaaaagtgat
     8041 gtggtgaagg gcaaagcgga aacggataag acgggcataa atgaagaaga aatggcgcgc
     8101 cctgcaggat tcgaacctgc ggcccacgac ttagaagttc ctagaacgac attttaagtc
     8161 aacaaattac cgcgccatct ctgcgctcac acgtcccact acctcaaaac atgtaaagcc
     8221 ttgcaagcca ttgtgaggcc ttatgtgtct cagttttgtc cctctttttt gtactaaaaa
     8281 acatagcaat tgaggataaa cctcatgcta ttttcgctta tatgccttta aaggcatggc
     8341 gcttaaatag ataaaaacac cacaaaagca taaaaaacca cacagtaaaa ccgcaacata
     8401 aaacaataac agataattaa accaaaaaca gatagcgcat tgtgataatc attcaatact
     8461 aaacaaaata taaacagtgg agcaatatgt gattgactca ttaagttaga tataaaaaat
     8521 acatattcaa tcattaaaac gattgaatgg agaactttta tgcgggcgaa acttctggga
     8581 atagttctga caacccctat tgcgatcagc tcttttgctt ctaccgagac tatatcgttt
     8641 actcctgaca acataaatgc ggacattagt cttggaactc tgagcggaaa aacaaaagag
     8701 cgcgtttatc tagctgaaga aggaggccga aaagtcagtc aactcgactg gaaattcaat
     8761 aacgctgcaa ttattaaagg tgcaattaat tgggatttga tgccccagat atctattggg
     8821 gctgcgggct ggactactct cggcagccga ggtggcaata tggtcgatca ggactggatg
     8881 gattccagta accccggaac ctggacagat gaaagtaggc accctgatac acaactcaat
     8941 tatgccaacg aatttgatct gaatatcaaa ggctggctcc tcaacgaacc caattaccgc
     9001 ctgggactca tggccggata tcaggaaagc cgttatagct ttacagccag aggtggttcc
     9061 tatatctaca gttctgagga gggattcaga gatgatatcg gctccttccc gaatggagaa
     9121 agagcaatcg gctacaaaca acgttttaaa atgccctaca ttggcttgac tggaagttat
     9181 cgttatgaag attttgagct aggtggcaca tttaaataca gcggctgggt ggaagcatct
     9241 gataacgatg agcactatga cccaggaaaa agaatcactt atcgcagtaa agtcaaagac
     9301 caaaattact attctgttgc agtcaatgca ggttattacg taacacctaa cgcaaaagtt
     9361 tatgttgaag gcgcatggaa tcgggttacg aataaaaaag gaaatacttc actttatgat
     9421 cacaatgata acacttcaga ctacagcaaa aatggtgcag gcatagaaaa ctataacttc
     9481 atcactactg ctggtcttaa gtacaccttt taacgaagtt aaccagattt tctccccggt
     9541 ggtattgtat aaccccgggg aatttgttta cagttacaag taggcaatat ccactaattt
     9601 tgtaagcatt taacctatgt cataacgtgt aatctcttac cgcaacacaa gacctcaata
     9661 tattcaaatc ttatctttat tccgatattc agagacacaa tgcgaaaaat aattactcat
     9721 ttcaaagttg ttttaacgtt acttctacca gtaaccgtat ctgcccagca gatacagtgg
     9781 caatcctgta tggccagtca attcaaccac tggtttggtg aggaaaaacc gtctcctgac
     9841 ttactatgtg gttatttgtc tgttccatta aaatatacag acacaggcgg agatgcttct
     9901 tatgaaaaaa aatcacaagt caaactagcg ttgacaaaat tgccggcaaa aagcaagcat
     9961 aaaggaagta tcctgataat aagtggtggt cccgggttac caggcataaa tccttatatt
    10021 aactttgact ggccagtcac aaatcttcgt gagtcatggg atattattgg atttgatcct
    10081 cgaggcgtcg gacagtccac tccgacaata aactgccggc aatcagatac agagactcag
    10141 gaaaacataa ccgaaaagca acaagtatta aataaaatta atgcctgtat ccataatacc
    10201 ggagccgaag tcattcgcca tatcggctct aacgaggctg tatacgatat tgatcgtatt
    10261 aggcaagcct tgggggataa acaactgaca gccgtggcgt attcgtatgg aactcaaatt
    10321 gcagccttat atgcagaacg ttttccctac aacgtaagat ctatcgttct tgatggagtc
    10381 gtcgatatcg atgacctgga ggacaacttc acatggcaac tcaaacaggc acagagttat
    10441 caggaaacgt ttgatcgctt tgcatcctgg tgtgcgcgta caaaaagttg cccgctttct
    10501 tcagacagag ataaggcaat aactcagttc catgagctat tatcaaaatt acatcacaaa
    10561 cctttattag acagtaaggg agaaaatata tcttcagatg aactcatatc attaacaaca
    10621 gaccttctgc tatggcgttc atcatggcca acccttgcaa ctgccatacg ccagttctct
    10681 caggggattg tcagtaatga aattgaaact gcgctcagtg ctccgatagc ctcagaagag
    10741 tcaagcgatg cttcgggggt aatcctctgt gtagatcagg gggatgagca attaacacca
    10801 gaagagcgaa aatcccgaaa agacgctctt gcgaatgcct tcccggctat taactttgac
    10861 aatggacgtt ccgattcacc tgatttttgt gaattatggc caatacatag cgacctgaac
    10921 aaaactcgcc tgaaaaatac tgttctgccc tctggtttac tgtttgtagc acacaaatac
    10981 gacccaacaa cgccctggat taatgcccgt aagatggcag agaaattttc cagcccgtta
    11041 ctaacaataa atggtgatgg gcatacatta gctctcaccg gagttaattt atgtgtagat
    11101 aaagcagttg tacattacct gatcactcca caaaaaatag aaaatatata ctgcccagga
    11161 aattctgaag cagaaataca ataatttcaa aatattccgc tatttgctcc caatgcaaaa
    11221 catatattgc attgggagca tattcatatt tttgttatta ttgtatgaaa tcgtctcatg
    11281 tacacggtcc ttttttcgcc gaaaactgat attactttaa tcaaaagttg acgtgccaga
    11341 ttcgaagtaa atttataaaa tatcaaatca atatacattt tgcaccttcg agatagtaat
    11401 gactgttact gtaacatgag ttacaaacag cttcataccc agagctactg atgtatcaac
    11461 tcgattcaac aatacccgat atttattcca tgcctccagc aacgatcttt cttcctccgt
    11521 tgcgatttcc agatctacag catcctgcag tggcgcaata tactcactga attcctggat
    11581 gtagaactgt gtggtgacgg tcttccagcc attcagctcc tgctgtatcg aagcatacca
    11641 ggctatttca atatcgctat gctgcggcag catttaaccc cttgtaattc atcgccataa
    11701 ttgatttaat tcacaaataa aactataaca tggtgaaatt aatgaaaaaa aacacagatg
    11761 atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg gttttgggag
    11821 tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca cactttctgg
    11881 aacatttagg taacaaccat ctggatattg gtgttggaac tgggttttac cttactcacg
    11941 tacctgagag tagtctgata tctttaatgg atttgaacga agctagcctg aacgcggcat
    12001 ctacaagggc tggggaatca aaaattaaac ataaaattaa ccatgatgtt tttgaacctt
    12061 atcccgcggc gttacatggt caatttgatt ccatttccat gttttacctt cttcactgcc
    12121 tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag gccttaactg
    12181 acgatggaac tctatacgga gccacaattc ttggcgatgg agttgtgcac aatagcttcg
    12241 gtcaaaaact gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca aaagattccg
    12301 aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc aaggttcaag
    12361 gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccaaccgca gcacgttctt
    12421 gcataaggcg tgtctgcggt ttttcaacta tttagataca tcactcccat cacattcatt
    12481 cctccgcatc ataggcatat aggctatatc accttgatat
//
LOCUS       DAZFYC010000142        12458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFYC010000142 DAZFYC010000000
VERSION     DAZFYC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.142"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            277..1665
                     /gene="narU"
                     /locus_tag="ACHKW5_004370"
     CDS             277..1665
                     /gene="narU"
                     /locus_tag="ACHKW5_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO7513618.1"
                     /translation="MALQNEKNNRYLLRDWKPENPTFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1747..5487
                     /gene="narZ"
                     /locus_tag="ACHKW5_004371"
     CDS             1747..5487
                     /gene="narZ"
                     /locus_tag="ACHKW5_004371"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO7513619.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5484..7028
                     /gene="narH"
                     /locus_tag="ACHKW5_004372"
     CDS             5484..7028
                     /gene="narH"
                     /locus_tag="ACHKW5_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7513620.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7028..7723
                     /gene="narW"
                     /locus_tag="ACHKW5_004373"
     CDS             7028..7723
                     /gene="narW"
                     /locus_tag="ACHKW5_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7513621.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7720..8400
                     /gene="narI"
                     /locus_tag="ACHKW5_004374"
     CDS             7720..8400
                     /gene="narI"
                     /locus_tag="ACHKW5_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7513622.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8479..9372
                     /locus_tag="ACHKW5_004375"
     CDS             8479..9372
                     /locus_tag="ACHKW5_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO7513623.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9468..10313)
                     /gene="nhoA"
                     /locus_tag="ACHKW5_004376"
     CDS             complement(9468..10313)
                     /gene="nhoA"
                     /locus_tag="ACHKW5_004376"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO7513624.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDGWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10486..11055
                     /locus_tag="ACHKW5_004377"
     CDS             10486..11055
                     /locus_tag="ACHKW5_004377"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO7513625.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDNKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11059..11286)
                     /gene="pptA"
                     /locus_tag="ACHKW5_004378"
     CDS             complement(11059..11286)
                     /gene="pptA"
                     /locus_tag="ACHKW5_004378"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO7513626.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11385..12458)
                     /locus_tag="ACHKW5_004379"
                     /pseudo
     CDS             complement(11385..12458)
                     /locus_tag="ACHKW5_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2962 a         3227 c         3193 g         3076 t
ORIGIN      
        1 atagttaata tgtaaccctt tagattcacg atctgaaggg ttgaaacatg acatacgttc
       61 aaagattaac ccccgtttga aggttagctt gttagttaca tttagtaaca catatctggc
      121 tacttaatcc ccattctgcc cccgcggtta cgctttctta tctattcttt caatcagcac
      181 ttcacccaat tcatccttaa ttctaataac tggctattgc gccttacctc ctgttttgtc
      241 ttttttcatt accaatgtgt gcatgtgagg aacaatatgg cactgcaaaa tgagaaaaat
      301 aatcgttatc ttttacgcga ctggaaacca gaaaatccga ccttctggga aaataaagga
      361 aaacatattg ctcgaagaaa cctctggata tcagtcagtt gtctacttct tgccttctgt
      421 gtctggatgc tatttagcgc agttaccgtt aatctcaata aaatcggttt taattttact
      481 accgatcaac tctttttatt aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt
      541 ccctactcct ttatggtgcc tatattcggt ggacgccgat ggacggtttt tagtactgca
      601 atcctgatta ttccttgcgt ctggctcgga attgccgtgc aaaatccgaa tactcctttt
      661 gggatattta tcgttatcgc tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc
      721 atgggcaata tcagtttctt ctttccaaaa gccaaacaag ggagcgctct tgggattaat
      781 ggcggattag gaaacttagg tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt
      841 gtacctgtat ttgcctttct cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg
      901 tcgctggcga atgccgcatg gatttgggta ccgctactgg cgattgccac gatcgccgcc
      961 tggtcaggga tgaatgatat cgccagttca cgcgcctcaa ttgccgacca gctccctgtc
     1021 ttacaacgcc tgcatctctg gctgctgagc ctgctttacc ttgccacctt cggttcgttt
     1081 atcggttttt ctgcgggttt tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt
     1141 ctgcgcctgg cgttctttgg cccatttatc ggtgccatcg cgcggtcggt tggtggtgct
     1201 atttccgata agttcggcgg cgtgcgggtg acgttgatca actttatttt tatggcgatt
     1261 ttcagtgccc tgctgttcct taccttaccg ggcacaggct ccggtaattt catcgccttt
     1321 tacgccgtat ttatggggct gtttctgact gcgggtctgg gaagtggttc tactttccag
     1381 atgatcgccg tcatctttcg ccagataacc atttatcggg taaagatgaa aggcggtagt
     1441 gatgagcaag ctcagaaaga agccgtcacc gaaacggcgg cggctctggg ctttatctca
     1501 gccattggcg cagtgggcgg cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg
     1561 accggctctc cggtcggcgc gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg
     1621 ctgacctggc tggtttatgg tcggcggaag ttcagccaaa aataagagat cgatgcctga
     1681 tgtacaaccg tgtcgcatcc gacaaggcat cagatcccaa ttcattcctg gagcaggagt
     1741 catgtcatga gtaaactttt ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt
     1801 gccgatggtc acggacaggt gatgcatagc aaccgcgact gggaggacag ctatcgccag
     1861 cgttggcagt tcgacaaaat cgtgcgttcc acccacggtg ttaactgtac aggctcctgt
     1921 agctggaaaa tctacgttaa aaatggtctg gtgacctggg aaatccaaca gaccgactac
     1981 ccgcgcactc gccctgacct gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt
     2041 tactcctggt atctttacag cgctaaccgc ctgaaatacc cgctcattcg taaacgactg
     2101 attgaactgt ggcgcgaagc cctcaaacaa cacagcgatc cggtactggc gtgggcatcg
     2161 attatgaacg atccgcaaaa gtgcctgagc tacaaacaag tgcgtgggcg cggcgggttt
     2221 atccgctcca actggcagga actaaaccag ctgattgccg ccgctaacgt ctggaccatc
     2281 aaaacctacg gcccggatcg cgttgccggt ttctcgccga tcccggcgat gtcgatggtt
     2341 tcttacgccg ccggaacgcg ttatctgtcg ctgcttggcg gcacctgttt aagtttctac
     2401 gactggtatt gcgacctgcc gcccgcctcg ccgatgacct ggggcgagca aaccgacgta
     2461 ccggaatctg ccgactggta taactccagc tacatcatcg cctgggggtc taacgtaccg
     2521 cagacacgta cgccggacgc ccacttcttt accgaagtac gctacaaagg cactaaaacc
     2581 atcgccatta cccctgacta ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg
     2641 aaacaaggca ctgatagcgc cctggcgatg gcaatgggcc atgtgatttt aaaagagttt
     2701 catctcgata atcccagcga ctactttatc aactactgcc gccgctacag cgacatgccg
     2761 atgctggtaa tgctggagcc tcgcgacgat ggtagctacg ttcccgggcg catgatccgc
     2821 gcatctgacc tggtggatgg actgggcgaa agcaacaatc cgcagtggaa aaccgtagca
     2881 gttaataccg caggtgaatt ggtagtgccg aacggttcga ttggtttccg ctggggagaa
     2941 aaaggcaaat ggaatctgga atccattgcc gccggtacgg aaaccgaatt gtcgttaacc
     3001 ctgctcggtc aacatgacgc tgttgcaggc gtggccttcc cctactttgg cggcattgaa
     3061 aatccgcatt ttcgcagcgt aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa
     3121 aacctgacgt tagtcgatgg caacacctgt ccggtggtca gcgtttatga tttggtactg
     3181 gcgaattacg gcctcgatcg cgggctggaa gatgaaaaca gtgcgaaaga ttacgctgaa
     3241 atcaaaccgt acaccccagc ctggggtgag caaattaccg gcgtgccgcg ccagtatatt
     3301 gaaaccatcg cccgtgaatt tgccgatacc gcccataaaa cgcatgggcg ctcgatgatt
     3361 atcctcggcg caggtgttaa ccactggtat cacatggata tgaactaccg tgggatgatc
     3421 aatatgctga tcttctgcgg ttgtgtcggg caaagcggtg gcggctgggc acactatgtc
     3481 ggccaggaaa aactgcgccc acaaaccggc tggttgccac tggcctttgc gctcgactgg
     3541 aaccgaccac cgcgccaaat gaacagcacc tcgtttttct acaatcattc cagccaatgg
     3601 cgctatgaaa aagtctctgc tcaggagtta ctttcaccgc tcgccgatgc cagtaagtac
     3661 agcggtcatc tgattgattt caacgttcgc gccgaacgta tgggctggct accttctgcg
     3721 ccgcagctgg ggcgtaaccc gctcgggatt aaagctgaag ccgacaaggc cggattatcc
     3781 cccacagaat ttaccgccca ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa
     3841 ccagatagcg gcagcaatca tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc
     3901 tcctccggca aaggccacga gtatatgcag aagtatctgc tggggaccga aagcgggatt
     3961 cagggcgagg aactcggtgc cagcgacggg atcaaaccgg aagaagtcga gtggcaaact
     4021 gcggcgattg aaggcaagct cgacctgctg gtgacgctcg acttccgcat gtccagtacc
     4081 tgcctgttct ccgatatcgt tctgcccacc gccacctggt acgaaaaaga cgatatgaac
     4141 acctcggata tgcatccgtt tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa
     4201 tcacgcagcg actgggaaat ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg
     4261 ggtcatcttg gcaaagaaac cgacgtggta ttacaacccc tactgcatga ctctccggca
     4321 gagctctcac agccgtgtga agtgctcgac tggcgcaaag gcgaatgcga tctgattcca
     4381 ggcaaaaccg cgccgaatat tgtggcggtg gagcgcgact accctgctac gtatgaacgc
     4441 tttacctcgc tcgggccatt gatggacaaa cttggcaatg gcggtaaagg gatttcgtgg
     4501 aatacgcagg atgaaatcga tttcctcggc aaactcaatt acaccaagcg tgatggcccg
     4561 gcacaggggc gtccgctgat tgacaccgcc attgacgctt cagaagtgat tctggcactg
     4621 gcaccggaaa ccaacggtca tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc
     4681 gggcgcgaac atacccatct ggcgctgcac aaagaggacg agaagattcg ctttcgcgat
     4741 attcaggcgc agccgcgtaa aattatctcc agccccacct ggtccggtct ggaaagcgat
     4801 cacgtatcct ataacgcggg atacaccaac gttcatgagt taattccgtg gcgcacgctg
     4861 tcgggacgcc agcagctcta tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg
     4921 gtggcttatc gcccgcctat cgacacccgt agcgtcagtg agatgcgcca gataccgcca
     4981 aacggcttcc cggaaaaagc acttaacttc ctgacgccgc accagaaatg gggcattcac
     5041 tcaacctaca gtgaaaacct gctaatgctg acgctctctc gcggtggacc gattgtctgg
     5101 atcagcgaaa cagatgcccg agaactgacc attgtcgata acgactgggt ggaagtattc
     5161 aacgccaacg gcgcgctgac tgcccgcgcg gtggtcagcc aacgtgtacc gccgggcatg
     5221 accatgatgt atcacgccca ggaacgcatt atgaatattc ctggttcgga agtaactggc
     5281 atgcgcggcg gcattcataa ctcggttacc cgcgtttgcc cgaaaccgac gcatatgatt
     5341 ggcggttacg cgcagctggc ctggggcttt aactactacg gcaccgtcgg atcgaaccgc
     5401 gatgagttca tcatgatccg caagatgaag aacgttaact ggctggatga tgaaggtcgc
     5461 gatcaggtac aggaggcgaa aaaatgaaaa tccgttcaca agtcggcatg gtgcttaacc
     5521 tcgacaaatg tatcggctgc catacctgtt cggtgacctg taaaaacgtc tggaccgggc
     5581 gcgaaggcat ggagtacgca tggtttaaca acgtcgaaac caaaccgggc attggttatc
     5641 cgaaaaactg ggaagatcag gaagagtggc aaggcggctg ggtccgcgat gtgaatggca
     5701 agatccgtcc gcgtctgggc agcaagatgg gcgtaataac caaaatcttc gccaacccgg
     5761 tggtgccgca gattgatgat tactacgaac ctttcacttt cgactacgaa catttgcata
     5821 gcgcaccgga aggcaaacat atccctactg ctcgcccgcg ctcgctgatt gacggtaagc
     5881 ggatggacaa agtgatctgg gggccaaact gggaagaact gctgggcggc gagtttgaaa
     5941 aacgtgcccg cgaccgcaac ttcgaggcca tacaaaagga gatgtacggg cagtttgaaa
     6001 acaccttcat gatgtacctg ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg
     6061 cgacctgccc aagcggcgct atctacaaac gcgaagaaga cggcattgtg ctgattgatc
     6121 aggataaatg ccgtggctgg cgtttgtgca ttagcggttg tccgtacaaa aaaatctact
     6181 tcaactggaa aagcggcaag tcagaaaaat gtatcttctg ttatccgcga attgagtcag
     6241 gccaacccac cgtgtgctca gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc
     6301 tttacgacgc cgaccgcatc gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg
     6361 aacgtcagtg cgaagtgttc ctcgatccac acgatccctc agtgatcgag gaagccctga
     6421 aacaaggtat tccacaaaac gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg
     6481 cgatggactg gaaactggcg ctaccgctgc accctgaata tcgcaccctg ccgatggtct
     6541 ggtacgttcc tccgctgtcg ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa
     6601 gcgaaggcgt gctgcccgcc atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata
     6661 tgttgagtgc cggcgatacc ggtccggtac tgcgggcgct gaaacggatg atggcgatgc
     6721 gccactatat gcgttcacaa accgtggaag gcgttactga tactcgtgcc atcgacgaag
     6781 taggcctgag cgtcgcccag gtcgaagaga tgtatcgtta cctcgccatt gccaactatg
     6841 aagatcgttt tgtcatcccg acgagccatc gggaaatggc gggcgatgcc ttcgcagaac
     6901 gcaacggctg cggttttacc tttggcgacg gttgccacgg ttcggacagt aaattcaacc
     6961 tgttcaacag tagccgtatc gatgccatca acatcaccga agtgcgcgac aaagcggagg
     7021 gcgaataatg cagatcctca aagtgatcgg cctgttgatg gagtatccgg acgagctgtt
     7081 gtgggaatgc aaggaggacg cgctggcgtt gatccgcagc gacgcgccga tgcttacgga
     7141 tttcacccac aacctgctta acgcgccgct gctggataaa caggccgaat ggtgcgaagt
     7201 gtttgaccgt ggacgtacca catcgctgct gctgttcgaa catgttcatg ccgagtcccg
     7261 cgatcgcggc caggcaatgg tggacctgct ggcggagtat gaaaaggtcg gcctgcagct
     7321 ggattgtcgg gaactgcccg attatctacc gctgtatctg gagtatttaa gcgtactgcc
     7381 tgacgatcag gcgaaagaag gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg
     7441 tcgcttaaaa caacgcgagg caccgtggta cgcgttgttt gatgctctgc tgcaactggc
     7501 gggaagctcc ctttcaagtg acagtgtcac aaaacaagta aacagcgaag agcgcgatga
     7561 cacccgccag gcgcttgatg cagtgtggga agaggaacag gtgaagttta ttgaagataa
     7621 cgccacggcg tgtgacagct cgccgcttaa tcaatatcag cgacgcttta gccaggatgt
     7681 cgcgccgcaa tatgtcgaca tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc
     7741 tttttttacg atatctaccc gtacatttgt gcgacggtgt ttttcctcgg cagctggctg
     7801 cgctacgact acgggcagta cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc
     7861 gggatggtta tatggtcgaa tctgttccat atcggcattt tggggatctt cttcgggcac
     7921 ctgttcggca tgttaacgcc gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa
     7981 caactaatgg cgatggtgct cggcggtatt tgcggtgtgt tgacattgat tggcggcgca
     8041 ggactgctgt ggcgcaggct gacaaatcag cgcgtgcgtg caacttccac tacgccggat
     8101 attatcatca tgagcatttt gctgatccag tgtctgctgg ggctaagtac cataccgttt
     8161 tcagcacagt atcctgatgg tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt
     8221 gtcactttcc gtggtggatc gtcagaaatg cttaacggcg tagcgttcgt tttccgcctg
     8281 catctggtat tgggaatgac catcttcctg ctcttcccgt tcaccagatt ggtgcatgtg
     8341 tggagcgcgc cgtttgagta ctttactcgt cggtatcaga ttgtgcgttc gcggcgataa
     8401 tttcaccctg gccggatagc ccttcgctct ccggccactt gcaggctatg atagttttcc
     8461 agtcacagga gaacgttaat gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa
     8521 ccatttcgcg gcaattctgc cggtgttgtt ttccccgccg ataatcttag cgaagcgcaa
     8581 atgcagctta tcgcccgcga gttaggccat tcggaaactg cttttctgct gcacagcgac
     8641 gacagcgatg tgcgcatccg ctactttacg ccaacggttg aagtgccgat ttgcggtcac
     8701 gcgacagtag ctgcgcacta tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc
     8761 tggcaaacat cgctggcagg aaaacatcgc gtgactatcg aaaagcacaa cgatgattat
     8821 cgtatttcgc tggaacaagg tacgccgggc tttgagccac cgctggaagg tgaaacacgt
     8881 gaggcgatta tcaacgcgct tcatctcact gaagacgata ttttgccggg cttgccaatt
     8941 caggtcgcaa caacaggaca ctcaaaagtg atgatcccgt tgaaaccgga agtggatatc
     9001 gacgcccttt cgcctgatct tgctgcgctg agcgctatca gcaaacagat tggctgcaat
     9061 ggtttcttcc cgttccagat ccgcccaggc aaaaacgaaa ccgatggtcg tatgttctcg
     9121 cctgcgattg gtattgtgga agatccggtg actggcaatg ccaatggccc gatgggtgca
     9181 tggttggtac atcacaacgt attgccccac gatggcaacg tgttatgtgt taaaggccat
     9241 caggggcgcg cattggggcg tgacggcatg attgaagtga cggtgacaat tcgtgataac
     9301 caaccggaaa aagtcaccat ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca
     9361 attgaactct gaaaccgttg cctgatgcgc ttcgcctgtc aggtctacaa ccgagctaat
     9421 gtatgccgga taaggcgttt acgccgcatc cggcaacctg acataaatta ttttcccgcc
     9481 tccggatgcg tatcaaacgc cgccatcacc agcgctaact catccacagt aaagccatgt
     9541 ttcgcatcat ccacgcccag accaaactgt tcctgcatca cggcatataa cgacaccaca
     9601 tccggtagat ttcgctgctc caccgcgtgc ccattttcat aatgggtaaa atgaaaattg
     9661 gtcagcgtta gcttgccacc gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa
     9721 tgcgactgcg gccagtgcgc cgaccagaaa ttgcccatca cataatcgct ttgttgctgc
     9781 tcgcagagat caaaacggta catcgactgc caatgctgat gatgattaaa ctgcaacacc
     9841 caaccatcac cctcctgcaa caaccgatac tctccgtgtg gcgtggtctg cacgagatcg
     9901 gaaactaaac gaatcggcgc ggttagcgtc tgcccaccga aaccgacatc agcaatccat
     9961 ttttcctctt ccagttccac caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga
    10021 tttgataaca ctacgcgccc taacaagctg cgaacgttaa accccagctc gcgtaacacc
    10081 cgctcaaaca cgccattctg ctcaaaacag taaccgccac gacgggctat caccagtttc
    10141 tcttccagcg attgatcatc aagctgtatt tccctcggca gcaaaacgtc aaggttttca
    10201 aacggaatag tgcaattgtg tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca
    10261 gcagctcccg accagttaat acgggcaaaa tagtgattca gaatgggcgt catcaggcgt
    10321 ttcccttagt ggttttctca acttataacc tggaatagac gccgcaccgg ctattttgtg
    10381 cgatcgcgga catgcatcac gtttgcaaat cgccaatccc gttaatttcg ttacttattg
    10441 cgttacggac tacgcttaaa aaaaccttct tcgcgaggcg ataccatgag ccgattcatc
    10501 cccatagaat tacaccatgc cagccgtctg ttaaatcatg gcccaactgt catgatcacc
    10561 agtttcgatg agcaatccca gcggcgtaac attatggcag cagcctggtc aatgccggtg
    10621 gagtttgaac cgccacgcgt ggcgattgtg gtagataaat cgacatggac cagggagttg
    10681 attgaacgta acggtaaatt tggcatcgtt atcccgggcg ttgcagcaac taactggacg
    10741 tgggcagtgg gaagcgtgtc gggtcgtgaa gaagataaat ttaattgcta tggcattccg
    10801 gtggtgaaag gtccggtgct tggtttgcca cttgttgaag agaaatgcct ggcgtggatg
    10861 gagtgccgcc tgctacctgc gacttctgcg caacaaaaat acgacacgct gtttggcgag
    10921 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    10981 aataagctca atacgttgca tcatttaggt gctggcacgt ttgttactag cggcaagcgt
    11041 gttacggcag gttaagcatt acatgctgta accgggtttc tttatcaaag cctccatttg
    11101 aggcgcgatt tcggcatccc agatagcttg ccaagattct ggttgaatct gctgtagagc
    11161 aatgcttatc gaactgtctt tactgttcag atggcgaata ataacgtcgg taatatctgc
    11221 agcaagtgct gctttttgtt gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg
    11281 cggcatgatt acgctccttt tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca
    11341 tgtgtccggt aaagcgttta tacgacaata atgccagggc aagattacga aagccttctc
    11401 cccgcaagga ctgacgccag gtagtttctg tccatggctg cttttcgcat cttacgtctt
    11461 aaccctgcct tgaatacctt atcattcgtc aaaatattaa tagcaatgtg ccgtatccct
    11521 gaaaataatt ctgctgcatt tccttttctt attttgcagt cgtcttcatt cattaccacg
    11581 tccagacgcc agtgcagctt attctccacg tgccagtggt ttcggattgc tgtggcgaac
    11641 ttctctgcgg ttaaatcagc agaactgata taatatctga ccgtcatttc tggctctttc
    11701 ttttgttctg ctattatgga ccgaaaggag actgccacgc ataatttctt cagccctttc
    11761 cattcaaacg tgaaatcaat aagttcatca gggacatcgc aaacaatatg aagacggatt
    11821 tcttctctgc cgtgactctt ttcactcatt gcgtaactgt catgctctgg attatttaat
    11881 tctttcagcg gaaatttttc ctcaaaggct ttatttagcc gcccctggtt tccttttacc
    11941 gcgaataaat aatcacctcc ctgtttttgt atcttttctg caatatcttt ctggcagccc
    12001 atcgcatcag ttgtgatgat ttttccttta atatccatca tgttaagaag ttcaggaatg
    12061 gctgtaatct cattggattt ctcatccgtc ttgatctgtc cgaggaccag actatgcatt
    12121 gttgagaacg cactaatgac atgaatcgct cccctgcgac gactcttgtc ataagagtgc
    12181 cggagcgttt ttccatcaat tgcaatgacg tctttatcat ctgaagaatg gcagtcacgc
    12241 atccagttaa taaagcactc gtgaaatttt gcaggactga tacaggatac aactctggca
    12301 atgatatcgt gaacaggaat accattttca aaatcaccat atcacttcaa aacatcggga
    12361 tgtgtttccc caaaatcctc tatatcttcc cagccttctg caccagaaat aatggcacaa
    12421 atagtcaaca gtagaatatc cgataattta tgttccac
//
LOCUS       DAZFYC010000143        12250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFYC010000143 DAZFYC010000000
VERSION     DAZFYC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.143"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..357
                     /gene="grlA"
                     /locus_tag="ACHKW5_004380"
     CDS             <1..357
                     /gene="grlA"
                     /locus_tag="ACHKW5_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO7513627.1"
                     /translation="DGEPLYILVSLWCKLQEKWISRNDIAEAFGINLRRASFIITYIS
                     RRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPVSTGPKRKNYRVGNGIVG
                     QSNIWNEMIMRRKKEN"
     gene            complement(743..1198)
                     /gene="cesD"
                     /locus_tag="ACHKW5_004381"
     CDS             complement(743..1198)
                     /gene="cesD"
                     /locus_tag="ACHKW5_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO7513628.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(1212..2750)
                     /gene="escC"
                     /locus_tag="ACHKW5_004382"
     CDS             complement(1212..2750)
                     /gene="escC"
                     /locus_tag="ACHKW5_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO7513629.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISNSVNDDFSGEIKNEKPMKVLEKLSRLYHLTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            complement(2750..3205)
                     /gene="sepD"
                     /locus_tag="ACHKW5_004383"
     CDS             complement(2750..3205)
                     /gene="sepD"
                     /locus_tag="ACHKW5_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO7513630.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPTHLFSDIGRIV"
     gene            complement(3211..3783)
                     /gene="escJ"
                     /locus_tag="ACHKW5_004384"
     CDS             complement(3211..3783)
                     /gene="escJ"
                     /locus_tag="ACHKW5_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO7513631.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(3785..4162)
                     /gene="sctI"
                     /locus_tag="ACHKW5_004385"
     CDS             complement(3785..4162)
                     /gene="sctI"
                     /locus_tag="ACHKW5_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO7513632.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(4246..4542)
                     /locus_tag="ACHKW5_004386"
     CDS             complement(4246..4542)
                     /locus_tag="ACHKW5_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepZ"
                     /protein_id="HFO7513633.1"
                     /translation="MDAANLSPSGRVLPLAATINGNNPVDEKTGVMQPENGTNRTIRI
                     LGGVLISAGALAAIGAGIASLCVEDPSQKLGLGIAAGVLGGVTTLGGGLAMKYA"
     gene            4726..5079
                     /gene="cesL"
                     /locus_tag="ACHKW5_004387"
     CDS             4726..5079
                     /gene="cesL"
                     /locus_tag="ACHKW5_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO7513634.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTF
                     QQHVQIIERVRRMTS"
     gene            5076..7103
                     /gene="escV"
                     /locus_tag="ACHKW5_004388"
     CDS             5076..7103
                     /gene="escV"
                     /locus_tag="ACHKW5_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO7513635.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKKTFGTGNNKN
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            7087..8427
                     /gene="escN"
                     /locus_tag="ACHKW5_004389"
     CDS             7087..8427
                     /gene="escN"
                     /locus_tag="ACHKW5_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO7513636.1"
                     /translation="MISEHDFVLERYPRIQRVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYRIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGDEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            8487..8807
                     /locus_tag="ACHKW5_004390"
     CDS             8487..8807
                     /locus_tag="ACHKW5_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO7513637.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            8800..9216
                     /locus_tag="ACHKW5_004391"
     CDS             8800..9216
                     /locus_tag="ACHKW5_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO7513638.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNGTIYLSITLNP
                     NNKNHIFLYHKKNYICDKLKEIFPDEPIEFSIEYEN"
     gene            9179..10096
                     /locus_tag="ACHKW5_004392"
     CDS             9179..10096
                     /locus_tag="ACHKW5_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO7513639.1"
                     /translation="MNLLNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREV
                     SGNGIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHS
                     NTIFFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNL
                     FITDVQAADLLCAISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCR
                     LIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            10127..10642
                     /locus_tag="ACHKW5_004393"
     CDS             10127..10642
                     /locus_tag="ACHKW5_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO7513640.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRCVQSTENLDYSNA
                     MISNSFSNSVV"
     gene            complement(10861..11220)
                     /locus_tag="ACHKW5_004394"
     CDS             complement(10861..11220)
                     /locus_tag="ACHKW5_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO7513641.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNEPSKIDKMLEINSNRDLLGMFFLSLKEDSVLLNIAFPADKVNITEFIN
                     LMENGYLLKNEVISLLS"
     gene            11471..12082
                     /locus_tag="ACHKW5_004395"
     CDS             11471..12082
                     /locus_tag="ACHKW5_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO7513642.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGGDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT         3608 a         2157 c         2534 g         3951 t
ORIGIN      
        1 gatggtgagc ctctgtatat cttggtttct ctttggtgta aattgcagga gaaatggatt
       61 tctcgcaatg atattgccga agcattcggt ataaacctga ggagagcatc atttattata
      121 acttatatat cgagaagaaa agaaaaaatt tcatttcgtg tcagatatgt tagttatggt
      181 aacttgcatt ataagcgcct tgagattttc atttatgatg ttaacctcga ggctgttccg
      241 atagaaagtc cggtatcaac cggaccaaaa agaaaaaact accgagtcgg taatggtatt
      301 gtggggcagt ctaatatctg gaacgaaatg atcatgaggc ggaaaaagga gaattaaacg
      361 tatttttttg agtatatttt atagaagaat aaaaataaag ctctattcaa gggctttgtt
      421 ttccaggata agcctttttc tctgccaaag tttgaaagtc atttcctgac aaatacactc
      481 tagggtatag atgattgaaa cagtcagatt tctgatgcta agttaagata tatactgctt
      541 attctataag cggaaatgac attgagtaca gcagaagatg gttcaggcgc ataaaatcct
      601 ggttaatgga gtaacccact cctgtagata aatctgccag cggtcggtgc tcaacgttgg
      661 gcgcacttca ccaaagcagc tttattattt ccgatagaaa atccccagac agcctgggga
      721 ttggtatggc agatggtagt ttttaatctg tattacctaa caatttttta atattttctt
      781 tatatgtagc gtatttttct tgttcattac accacattaa acaagcctta aaggcttttt
      841 tggccatttt ccgatttccg accaataagt agctaatccc agaataataa gatgccctgg
      901 gatcttgcat aactaaagtt gcacagcgag cgaaacacag ttgtgcatct tcatgatttg
      961 ataaacgctg atgacacaac cccagagata gggtgtagtc ataattccag tgctccagat
     1021 atgttagcag atgaaataga tttcttgcgg ttataacatc accagattga tatgctgtat
     1081 aggcataaga atgaagatca ttgagatctt gttgcgtcaa atttgtcagt gatgctaacg
     1141 taccaccatc ctggtagaaa tcatagatat cctgtagaga gctaaatttc ctgctcataa
     1201 agcctcattt attattcgct agatgctgat tttatcgggg tcgctttaat caaaaagagt
     1261 cgaacaacgc tgtgtttttg ttttgttgta ctggagaata agttaccaat aacaggaata
     1321 tcacttaaca acggtatacc atctttactt gaactctctt tatcctggat aaatccgcca
     1381 agcagcagac tttctccagc gcttaatgtc gcttctgttg tcatttctga attttgtact
     1441 tcaggtaaga tgctgctggc atcctgagca ttactttggt tagtcgattg attgccatcc
     1501 tgaatatcaa gtaacaaacg aactcgttcc cgacgttttc cggtaagact attcgagttg
     1561 tcatcaagta tacgtggtgt cactctcagt aatgttccgc tggtaatact ttccagagat
     1621 gccacctttt ctccggaaac ttttgtataa aacgttacgt ttttatcaag aatcgcctgc
     1681 atattattta aggttataat tgatggctga gagagtattt ttgctttcga gttctgttgt
     1741 aacgcattca ctctgatcat gaaattattg gcgttgctta tcacagaaga actgagatta
     1801 gcttgcgccg ttgagctgtt aaaggcaata gaaccttgcc ctgcatttaa tgtaccggac
     1861 cagtttactc ccagttgctg aagatcattt gcatcaacat caataattga aacagaaatc
     1921 tcaatttgcc tttgctcaat atcaagctca gaaattaatt gccgatatat gtccatagtt
     1981 atttcacgat cttttacaac aactgcatta agacgtggat ctgcggaaat ctttacactg
     2041 ttatcaaaca ggttgccgtt tctttctaca gcacctttgc ctgtgctggg aagtgagcca
     2101 ttacttgcca tcgtcttcag gatggagacg acgccaggca cgacaacatt ttggtcgcgg
     2161 tatttatacg ttatatctgt cgctgatgca tagtttaatt taaatactct aacgacatct
     2221 ttattttttg ctttagactg tgcttcttta tcaagacttt cagataaaga tgtaatgtat
     2281 tttatgcatt caggaacgcc acgaacttca atcgagttaa atgttgtgat ttttctcacg
     2341 ttacaggagt ttttatttac tgagattgta tcacgcagat attttaacag tgagtcaata
     2401 tccagatatg ttggcgtaat tatgcttctt gatatttcat ttgttttata aatatacaaa
     2461 atgttctcat cataatacca tgtgagatga tagagcctgg acagtttttc cagtactttc
     2521 attggttttt cattttttat ttcaccagag aaatcatcat tcacagaatt actgatgaaa
     2581 acagggatgc tataattagc ggcaaaatca ttgaggattg cacgtaccgg agagcttttt
     2641 gtaataataa aatactcgtt cttgcctaat cttttttcca gagaggacgg ggcagcctga
     2701 gcactgcagc aaaatagtgc tgtaaaaata aaaaaactta tttttttcat tacacaattc
     2761 gtcctatatc agaaaacaaa tgtgttggag gcatcgttgt atctgatgta gatgcttgct
     2821 ctaacttatc aaattgcttg tcaagcaaag aggcgagtgt gtgttgcatt attgcttttg
     2881 acatgtttgc gcgatgatta ttatctttat taaacagttg gcaaagatac cattttttat
     2941 tagattgaaa aatgcccatt tgtttatatt ctttactaac ggccagaata ttacagagtc
     3001 tgatgaaggt atctgttgga acctggcgaa aatcgccatt aagctcgata tacagaaccc
     3061 agtaacttcc aaagtcgtgt aagaaggcat ttttttgata gatatgaaaa gtaagatgct
     3121 ttggtgaacc tttgacatct tttacaaaat caagagccgt tagccagtca ctatcattct
     3181 taacaatgcc attattattg ttcatacata ttacccgtcc tgtcctgagg atgacttgat
     3241 aacgacacta atattttcta gtttgagatc gtctacgcta ttcttaacta agttttttat
     3301 ctgaatgact gatggtgcta agttaacctc tggcgagcta atgactaaaa cagccgctga
     3361 ggatggttgg ctctcgttat tgttaacatt taatgaaacg ctgcaatcga taacaccggg
     3421 gattttactt agtaaccttt ctatatcttg ttctttgaga tagttgattt ttgcattttc
     3481 ttgtgatggc gatgccacta gctgactggg cggaaaaata acttcaatat ctgcaaattt
     3541 ctttttcggg aagccattgt tatttaaaat ggttatcgca cggacaaaat cctctttttc
     3601 cacacttatt gttacatttc cacttttgtc catttcttta gatacattga catcattgga
     3661 taacaacaat gcttgcatct gattggcttc tttttccgtt aatcctgtat acaattgctc
     3721 tttacatgca actgtcaaac aaataaaaac aaataaaaag gtgtttttaa tgtattttct
     3781 cattttattg cattgaaacc agttttgtaa ccgatgttga aagaagactt gctaacttgg
     3841 atacgacatc aacattgacg tttgcgttga gcacattctt ttgtaatgtg agcacatctt
     3901 ctggcgagag tccacctgca gatagggcat cgaaactgag atcggtgttc attgttgcaa
     3961 gatgtctctt tttaatttct tcaattaaaa cttgctctgg agaagataca ccagctgttt
     4021 taggaagtaa ttgatcaaga aactgagccg ctccagctgc agagggcgtt ggtgctaacg
     4081 gggtatggtc atcaacgaaa tttgtttggg atactttatt aaaatgaata ttaatatttt
     4141 ctgattgatt aatattgttc atatttacgg ttagtctctt ttcatgagag aggtaatggc
     4201 ataatgcatc catgacctct ctattttatt tatgcgatgc gatgattagg catatttcat
     4261 cgctaatccg ccgccaagag ttgttacgcc acctaataca ccggccgcga ttcctaagcc
     4321 taatttttgt gaaggatctt caacacacag tgatgcgata ccagcaccga tagcggctag
     4381 agcacctgca cttatcaaaa ctccacctag gattctaatg gtacgatttg ttccattttc
     4441 tggttgcatt actcctgtct tctcatccac tggattattt ccattgattg tggctgccag
     4501 tggtaatact ctgccagaag ggcttaaatt tgctgcatcc attaactttt ctccattgtc
     4561 taatttagac atttgtctgg ttatcaataa ccaaattatc ccatgggcaa ctattatatt
     4621 atttaaatag ttgcaatgta cataaagtac atatactgta tatattatcc aacacgcttt
     4681 ttcaagcaat aatatccaca catatactta cttcagagcc tgcatatgaa tcttttaatt
     4741 aaaagaaacg tagaagagtt tttaaggtta ttagggaatg atttttattt gtttgataat
     4801 cgcgtggaaa ttgattttaa tggtttttct ttttttattg aaataattga tagtaatgtg
     4861 tttattacgt ttgccttaga gtataacgaa aatgcgtttt tttctttttt caatgccctt
     4921 gcccctgaac ggacgcaggg agtgattgaa catatttttg tctacgataa caaattgtgc
     4981 ttaagttgtt tgttaaccaa tattgatgtg ttttctctaa tgaatacttt tcagcagcat
     5041 gtgcagatta ttgaacgagt tcgcaggatg acatcatgaa taaactctta aatatattta
     5101 aaaaagcaga gtcatatcac gatcttattc tggctctctt cttctttatg gctgtaatga
     5161 tgatgattat tccattgcca acagttgtgg tggatatcat tatcgcgatt aatatatcga
     5221 cagcattgct tttattaatg ctctcaatct atataaaaaa tcctcttgag ctaacttctt
     5281 ttcctacaat cctgttgatt acgacgttga tgcgcctgtc gctcagtgtt agtacaacca
     5341 gacttattct gctgcatcac gatgcgggtg acattattta ctccttcggt aactttgttg
     5401 ttggcggtaa tattgttgtt ggcctggtca tttttaccat tatcaccatt gtacaattta
     5461 tggttattac aaaaggggct gagcgtgttg ctgaagtgag tgctcgtttt tctcttgatg
     5521 gtatgccagg aaaacaaatg agtattgatg gtgatatgcg tgcaggcgtt attgacccgc
     5581 ttgaggcaaa agttcttcgc tcccgagtgc aaaaggaaag ccagttttat ggttcaatgg
     5641 acggggcgat gaagtttgta aaaggggacg ctattgccgg tatcatcatt gttttagtta
     5701 acctgtttgg tggcgtgctc attggaatgt ggcaatttga catgccattt agtgaagcac
     5761 ttagcctgtt ttctgtattg tctgtcggtg atgctttggt tgcccagatc cctgcgctta
     5821 ttatttccgt cactgcgggg gttgttgtta ctcgtgttcc cggtgaaagc gaaaaagaag
     5881 aaaaccttgc aggtgatatt gttcaacagg tttctgttaa tagtcgccct tttttaatca
     5941 gtgccgcact catgatcgta atggcgatta ttcctggctt tcctacattg gtcttcttgt
     6001 ttctggcggt ttgcctgttg ggtatcgcct ggaaattaca aaagaaaaag acgtttggaa
     6061 ccggaaataa taagaacgct atgggagctg atttgtctaa tagtcaaaat atctcaccgg
     6121 gtgccgagcc gttgatttta aacttaagta gtaatattta tagctcagat attacacagc
     6181 agattgaggt tatgcgttgg aatttctttg aggaaagtgg aattccattg cctaagatta
     6241 ttgttaatcc agttaaaaac aatgatagcg ctatagaatt tttgctctat caagagtcaa
     6301 tatacaaaga tactctgaca gatgatactg tttattttga ggctgggcat gcagagatat
     6361 catttgaatt tgtccaggaa aagctttcgg ctaactctat cgtgtataaa acgaatgagg
     6421 ctaaccaaca actagctcac gtctcaggta tggatgttta tgcaaaaaca aatgataaga
     6481 taacgttttt gctaaagaaa cttgtattgt ctaatgccaa agagttcatc ggcgtacaag
     6541 aaacgcgtta tctgatggac atcatggaga ggaaatataa cgaacttgtg aaagagcttc
     6601 agcgtcagct tggtctgagc aagattgtgg atattcttca gcgtctcgta gaggaaaata
     6661 tctcaattag agatctacga actatttttg aaacgcttat tttttggtcc acaaaagaaa
     6721 aagatgtggt tatcttgtgc gaatacgttc gtatcgccct acgtcggcat attttaggtc
     6781 gctatagcgt tcgcggtaca cttctgaatg tttggcttat tggctctgat attgaaaatg
     6841 agctacgaga gtcgatcaga caaacgtcat caggttcgta tttgaatatc tcaccggagc
     6901 gaagtgagca gattattggc ttcttaaaaa atattgtgaa tccaacgggg aacggcgtca
     6961 ttctgaccgc tttagatatc aggcgctacg tgaagaaaat gattgaaggt tcgttcccgt
     7021 cagtccccgt gctctctttt caggaagttg ggaataatat cgaacttaaa gtattaggaa
     7081 cggtaaatga tttcagagca tgattttgta ttggaaagat acccacgtat ccagagagtg
     7141 ctcaatagca ctgtgccgac attatcatta aattcgtcta ccagatatga aggcaagatt
     7201 accaatatcg gcgggacgat tatcaaggcg cgtttaccaa aagcgcgtat tggcgccttt
     7261 tacaggatag aacccagtca acgtttagct gaggtgatcg ctattgatga agatgaggtt
     7321 ttcctgctgc cttttgagca tatttccggc atgtattgtg gccagtggct aagttatcag
     7381 ggggatgagt tcaagattcg tgttggggac gaactccttg ggcgactggt tgatggtata
     7441 ggcagaccaa tggggagcaa tattactgca ccttatcttc cgtttgaacg cagtttgtat
     7501 gctgaacccc ccgatccttt attaaggcag gttattgatc agccatttac gcttggcgtc
     7561 agggcaatag atggtttact tacttgcggt attgggcaac gtatcggtat tttcgcgggg
     7621 tccggcgttg gtaaaagtac gcttttggga atgatttgta atggcgcatc cgcggatatt
     7681 attgtccttg cccttatcgg cgagcgtggt cgcgaagtaa atgaattcct cgcgctttta
     7741 cctcaatcca cgctttctaa atgtgtactg gtcgtcacaa cgtcagaccg tcccgcgctg
     7801 gaaagaatga aggccgcatt tacggcgaca accattgcag agttcttccg ggatcagggc
     7861 aaaaatgtat tattaatgat ggattccgta actcgttatg cccgagccgc gcgtgatgtt
     7921 gggcttgcat cgggagagcc tgatgtaagg gggggattcc ctccgagtgt tttttcctcg
     7981 ttacccaaat tattagagcg tgcggggcct gcgccaaaag gctcaataac cgcgatttat
     8041 acggtgttgt tagaaagcga taatgttaat gatcccattg gtgatgaagt ccgctctatt
     8101 cttgatgggc acattgtgct tacccgagaa cttgcagagg aaaatcattt tcctgcaatt
     8161 gatattggtt taagcgccag tcgtgttatg cataatgttg ttacatcgga gcatttgcgt
     8221 gcggcggcag aatgcaaaaa gcttatcgca acctataaaa atattgagct gcttattcgt
     8281 attggtgagt acacgatggg gcaagatcct gaagcggata aggcaataaa aaacaggaaa
     8341 gcaattcaga gctttataca acaaagcacc aaagatatca gtagttacga aaaaacgatt
     8401 gaaagcctat tcaaagtggt tgcctgaata tgttggacag aattttatct attcgtaaaa
     8461 gcagagcgaa tcgattgaga gaatcaatgg ctaagattaa ctctcagata aaagaggtgg
     8521 atggtaaact cgatgactgc gagcaagcca ttaaagagtc gattgcctct aagcaggcgt
     8581 attgtgcatc gctggtgaat ctggataaag tgtcgttgta taaataccag attaaaaaca
     8641 atgcatttga tgagcaaaag cagcgattat atgagaaaaa atcctcactt tctaaagaaa
     8701 agcgttcatt gctcgattca caaaaacgaa caaaagagga tctacaacat gttaataaat
     8761 caatagagaa gttatctttt gccataaagg aacattactt tgactagcat ttctctaaaa
     8821 agaaatttga tagagcctgt ttcttttcag ccgtcagatg attggccgca gggagaagaa
     8881 agttttaatg atatttttaa tcgcataaaa aaaagaacac ctgacacttc gttcatacga
     8941 atggcttctt tatggaaacg attgttctac gctcctggac agaggcgacg ctactttgaa
     9001 caaggagagc gttcatttag tattatttgt ggaaagttac gcggaattgt tttaacaata
     9061 aaatgtagta atggaaccat ttatctgtct attacactga accccaataa caaaaatcat
     9121 atttttttat accataaaaa gaattatatt tgtgataagt taaaagaaat ctttcctgat
     9181 gaacctattg aattctcaat tgaatatgaa aattaatgat ttttacttgc cattgttgtc
     9241 agtcatagga accggtcgcc tctatattac gcctgaaggg catgcttgcc atgcatattt
     9301 tcgcgaagtc agcggtaatg gtatccgctt cacgttaaca tgctcaggct atgaggggcg
     9361 tttttggata tcagaagaac agttcattca gtggtgccag gagttattcc cgtatagcga
     9421 aagccggctg ataccggaag atatgataaa actcatgatc ttatgggtaa tgcagactgc
     9481 tttaccagaa ggtgatgtgt ctgttgatga tgttcaattt acgatgctaa ataaagatgt
     9541 ttatccggta atagaaaata ataatggaga aaacagactt aatgtaatta tactcgaaat
     9601 aacggtacag agtttacaat atctaattaa tgaaaattgg caatttgttc cacattctaa
     9661 taccattttc tttgatgggt atattgctcc tgggtggact gattacccgg ttacagagct
     9721 gactgttggt gatagtttaa ggctctatca tgttgatgac agcaaagaaa gagagtgttg
     9781 gcttgttatt aataaccctt tagcaaccgt aaaactttgt gataataatt tatttattac
     9841 ggatgtccag gctgcagatt tgttatgtgc tatcagcaat gaggctgtaa tggatcgcat
     9901 ttattgtgtg attggaacaa ttcatgttga tatacatatg ttgcgcaatg ccaaaaaaga
     9961 tgatattatc gattcaactg gatatcatct ttttggtggg tgccggttaa tacgcaataa
    10021 tacgactata gcatacggga gcattgtaaa aatcaacgaa gatttttatt ttactgttag
    10081 tttagtatgt gattaatcag gaataagtta tatagggaga tgtatcatgt cgtcatcgtt
    10141 atcaggaata atactcaata cttcgctgac aagccaccat atgtcatggg gcaagtttag
    10201 cagaactctt catgtattga atgaagatat cgtagatgaa attcagctta aagcccgaac
    10261 acaacaacgc caccatcgag tatattcaga aatggcggat tactccgttg tctcctttcg
    10321 gggcaaagaa tattctgttc gttttattaa agattacaaa acagataatt atgtgtacaa
    10381 agtacaaaag atgactcagg aaagtgggtg tctctcaaga ttatttagct ttctttctgg
    10441 cggtgtgact aaggctttgg aaagtaaact aaatgagagg catatcactc cactttctcc
    10501 aacctggttt cctcgaactc cactggaggg ggtcttaacg gaaagaggat tatcttcttt
    10561 attacgttgc gtacagtcta ctgaaaacct tgattatagt aatgctatga tatcaaactc
    10621 tttctctaac tctgtagtgt gattatcatt tgagctttcc tcctggttta actccagaat
    10681 aattgtgatt ctggagtttt tctgtttgtt ttatgtcgat aatacattgt ttttaggaaa
    10741 atatgggatg aatattcaga gtgagtttgg ctggcagaag tcagatattc tgtgatgagg
    10801 aggggattga gctgaatgga tgaattcctt ccagctcata atttatcagg ataaaacatc
    10861 ttacgacaat aaactaatta cttcattctt taataaataa ccgttttcca ttaaattgat
    10921 aaactctgta atgttaacct tatctgcagg gaatgcaata ttcaaaagta cgctatcttc
    10981 ttttaatgat agaaaaaaca taccgagtag atctcgattg ctatttattt ctagcatttt
    11041 atcaattttt gaaggttcat tattgaataa ataacaataa aaaaacagga cctcagattg
    11101 aggcgcacac tccagtatcc attcatgacc atcgtgatca tggaaagaga gggcgttagt
    11161 ttgttcttca agagttattc cgaacgtatg tgcaagatct gtgcgaaatt gttcattcat
    11221 attgtagtcc tgtcattctt tcaatgaggg tatgcttaaa cgtaattaac tcgtgagttc
    11281 tggcacaaat aataagacga aaatataaga tgcgagtaat agtgttgcat atttatgata
    11341 gcatgtgata aatgtagtgc aactacttgt ggatagtgcc ggaggaaaat ttaaatataa
    11401 aacaattatt gtttttaatg aaaatgaaaa atattttgtt atcgttatat aatatataaa
    11461 ggtttattgt atgtttagtc caatgacaat ggtaggtaga tcgttagctc aggcggctac
    11521 acaaactctt agacctgcgg tcaccagagc tgcgacacaa gcgggggtgg gagccagtgg
    11581 tatgaagttc gtgcccatgc agtcgaactt tatgattaat catggcaaac tgactaacca
    11641 gctacttcag gccgtggcca aacaaacccg tagcggtgat actcagcaat ggttcaaaca
    11701 ggagcaaatt acttatatat ccagaacagt aaacagagct attgatgact attgcatgag
    11761 taataattcg gtaattggta aggagaccaa aagccacatt ttcaaggctg ttgaaagcgc
    11821 gataaagcag cctctcgata tgaattgtgc acaaacgtcg attggccatt tcttacagtc
    11881 caacaaatac tttaataaaa aagttgatga gcagtgtggc aaaggggggg atccaattac
    11941 acgctttaac acccagacca ggatgataga acaagtctca caagaaatct ttgaacagaa
    12001 atttggtggt ttgaaagtta gtgatatcaa agctctgact cagagcacga ttgcacagca
    12061 tgtacaggat actcggttgt aggaataacc tgttatatat gcgtgttggg cataagttaa
    12121 gcttcctggt gtatagcgta gtctttgctg atgtaagaaa aggctatata cggaaacttg
    12181 atatactata aacttcatta tttattatat tgaaaaagtt atttaattga tttgttttgt
    12241 aattttaatg
//
LOCUS       DAZFYC010000144        11743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFYC010000144 DAZFYC010000000
VERSION     DAZFYC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.144"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..196
                     /locus_tag="ACHKW5_004396"
     CDS             8..196
                     /locus_tag="ACHKW5_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513643.1"
                     /translation="MSHDGLFALAQKKMPDATLRVLYSHICQIQQRIRLPQLAHFHTC
                     PPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHKW5_004397"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHKW5_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO7513644.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /gene="yfiM"
                     /locus_tag="ACHKW5_004398"
     CDS             complement(1608..1931)
                     /gene="yfiM"
                     /locus_tag="ACHKW5_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO7513645.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHKW5_004399"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHKW5_004399"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO7513646.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHKW5_004400"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHKW5_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO7513647.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHKW5_004401"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHKW5_004401"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO7513648.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHKW5_004402"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHKW5_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO7513649.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /gene="yfiF"
                     /locus_tag="ACHKW5_004403"
     CDS             7531..8568
                     /gene="yfiF"
                     /locus_tag="ACHKW5_004403"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7513650.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHKW5_004404"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHKW5_004404"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO7513651.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHKW5_004405"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHKW5_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO7513652.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHKW5_004406"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHKW5_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO7513653.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /gene="yfiE"
                     /locus_tag="ACHKW5_004407"
     CDS             10739..11620
                     /gene="yfiE"
                     /locus_tag="ACHKW5_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7513654.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2920 a         2932 c         3037 g         2854 t
ORIGIN      
        1 aaaggccatc agtcacgatg gcctttttgc gttggcgcag aaaaaaatgc ctgatgcgac
       61 gctgcgcgtc ttatactccc acatatgcca gattcagcaa cgaatacggc ttccccaact
      121 tgctcacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataatca
      181 ttttcctgct aactaaattc atggttaagg ttgcataatg atatgcaaca aatgtataac
      241 attttattta ccaaaagaaa taaacaaaag tgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc gattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acattttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgttatcgg tgtgtatgat gtgtttcgga tcgctggtta tcgcctgcct cccaggttat
      661 gaaactatag gtacgtgggc tccggcatta ttgcttctcg ctcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac cacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgccaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggtctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagctccag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttta ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gatcagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccggattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgccgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataatc
     4861 gtcgtgcctc ttgcgaggaa tgttcgccac accagttcgt cagcagagaa acatacttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt aatatagtgc tcactgctgg cgtcatcgcg cagatcgagc gggttagaga
     5101 ttgccacatg tcctggcagt gcatcgcgca gtttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cctcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgctggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga agcgggaaag accaccattg gctgatagtc cgggttttgc
     6541 accaactcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga cgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcgccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcatgtca ggcacttttc cagagccgtc cggaagcgat tgttcgcgcc
     7921 tggtttatcc agagtgtaac gccgcgtttt aaagaagcct tgcgctggat ggcagcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagcg gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaatggca caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgtgtactg gcgctggaaa acgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggcgttgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgttggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggg aaaccgctgt tcaaaaccag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtt
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg cattttaccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gcaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccgca aattgcagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa cgaaccgcaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc tgttaagcat
    11401 tgaaagtatc aaacgttgtg tcgcggcgaa tatcggtgtc agttacttgc cccgctttgc
    11461 ggtggaaaaa gagcttgaga gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gag
//
LOCUS       DAZFYC010000145        11683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFYC010000145 DAZFYC010000000
VERSION     DAZFYC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.145"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            353..1123
                     /gene="yjjP"
                     /locus_tag="ACHKW5_004408"
     CDS             353..1123
                     /gene="yjjP"
                     /locus_tag="ACHKW5_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO7513655.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1114..1587
                     /gene="yjjB"
                     /locus_tag="ACHKW5_004409"
     CDS             1114..1587
                     /gene="yjjB"
                     /locus_tag="ACHKW5_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO7513656.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1694..2233
                     /gene="dnaT"
                     /locus_tag="ACHKW5_004410"
     CDS             1694..2233
                     /gene="dnaT"
                     /locus_tag="ACHKW5_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO7513657.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2236..2973
                     /gene="dnaC"
                     /locus_tag="ACHKW5_004411"
     CDS             2236..2973
                     /gene="dnaC"
                     /locus_tag="ACHKW5_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO7513658.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSSTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3022..3516
                     /gene="yjjA"
                     /locus_tag="ACHKW5_004412"
     CDS             3022..3516
                     /gene="yjjA"
                     /locus_tag="ACHKW5_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO7513659.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3770..6061
                     /gene="opgB"
                     /locus_tag="ACHKW5_004413"
     CDS             3770..6061
                     /gene="opgB"
                     /locus_tag="ACHKW5_004413"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO7513660.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSSQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6202..7224)
                     /gene="lgoD"
                     /locus_tag="ACHKW5_004414"
     CDS             complement(6202..7224)
                     /gene="lgoD"
                     /locus_tag="ACHKW5_004414"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO7513661.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7363..8277
                     /gene="lgoR"
                     /locus_tag="ACHKW5_004415"
     CDS             7363..8277
                     /gene="lgoR"
                     /locus_tag="ACHKW5_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO7513662.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8492..9853
                     /gene="lgoT"
                     /locus_tag="ACHKW5_004416"
     CDS             8492..9853
                     /gene="lgoT"
                     /locus_tag="ACHKW5_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7513663.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVTNLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9902..11566)
                     /gene="tsr"
                     /locus_tag="ACHKW5_004417"
     CDS             complement(9902..11566)
                     /gene="tsr"
                     /locus_tag="ACHKW5_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO7513664.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQCTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQDDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
BASE COUNT         2852 a         3078 c         2834 g         2919 t
ORIGIN      
        1 taatttataa ttaaatgata tcccttcccg cagaatgcgg gaatcacaca atcaatattt
       61 attcaataaa atctgatgat tttaacttgt taacactaaa aaacgcgccc agctatctat
      121 tatcctgacc cgtaaaggct aaaacaataa attcattata gtgataatat tcatgtgata
      181 gtttgtttgc ggcgagagat aattcgcttt ttatcaccga gcttgtcccg ataaccagga
      241 ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca tgatacaaat
      301 ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag ttatgcaaac
      361 tgagcaacag cgagccgtaa cgcggctttg tatccagtgt ggattatttc ttttgcaaca
      421 tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg cgttgggaat
      481 ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacta ttaaagatgg
      541 gcaatgcctg acatcgacac gaaaaaatca cgatcgcggc attaatatgc atgtggtgac
      601 tgaagtccag cacattgtga ttcttgcgga gcatcatctg ctggattaca aaggcgtaga
      661 gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag ccttaatggt
      721 tggcctttct tgcgcctgtt tctgtaaact caataacggt ggctgggatg gtgccgtcat
      781 caccttcttt gccagtacga ccgcgatgta tatccgccag ctgctggcac aacgtcatct
      841 tcatccacag atcaactttt gccttaccgc tttcgccgcc accaccattt ccggattgct
      901 tttgcaactc cccactttca gcgatacacc caccattgcg atggccgcca gcgttctgct
      961 gctggtgccg ggctttccgt tgattaatgc cgtcgccgat atgtttaaag gccacatcaa
     1021 taccggactg gcacgctggg cgatcgccag tctgctgacg ctggctacct gcgtcggcgt
     1081 ggtgatggca ctgacgattt gggggctacg cggatgggtg tgatcgagtt tctgtttgca
     1141 ttggcgcagg atatgatcct cgccgccatt ccagcggtcg gctttgcgat ggtgttcaac
     1201 gttcccgtgc gggcgttacg ctggtgtgcg ctgcttggcg cgattggcca tggttcacga
     1261 atgatcttaa tgaccagcgg gctgaatatt gagtggtcaa cttttatggc ttctatgctg
     1321 gtcggcacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa agtgtttacc
     1381 gtggcggccg ttatccctat gttcccgggc atatcggctt ataccgcaat gatttcggcg
     1441 gtaaaaatca gccagttagg ttatagcgaa ccgttgatga ttaccctgtt aaccaacttt
     1501 cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc tggattatgg
     1561 ttgtaccgca agcgccctcg cgtataaaat tgcctcgctt aagcacacgg atgagagaca
     1621 gcctcctctc ctccgtgtgt tactataaaa gttatctccc ttctcgttca tcattccata
     1681 tttgagaaac agtatgtctt ccagagtttt gaccccggac gtcgttggta ttgacgccct
     1741 ggtacacgat caccaaaccg ttctggcaaa agctgaaggc ggtgtggttg ccgtatttgc
     1801 taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc ctggctgaac tgctggcgct
     1861 ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg gacgatcaac tctatcagga
     1921 accgcaagcc gctcccgttg ctgtacccat ggggaaattc gccatgtatc cggactggca
     1981 acccgatgcc gactttatcc gcctggcggc gctatggggc gtggcactaa gagagccggt
     2041 gaccaccgaa gaactggcct cattcattgc ctactggcag gcggaaggta aagtctttca
     2101 ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa atcggtcgtg ccagcaacgg
     2161 cggactgccg aaacgagatg tgaatacggt cagcgaacct gacagccaaa ttccaccagg
     2221 attcagaggg taacgatgaa aaacgttggc gacctgatgc aacgcctgca aaaaatgatg
     2281 cctgcccata tcaaacctgc attcaaaacg ggtgaagagc tactggcctg gcagaaagaa
     2341 caaggggcga tccgctccgc cgctctcgaa cgtgaaaatc gggcgatgaa aatgcagcgc
     2401 acctttaacc gctccggtat tcgtccactg catcagaact gctcctttga gaactatcgc
     2461 gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc gccagtatgt cgaagagttt
     2521 gacggcaaca tcgccagctt catcttttct ggtaagccag gaaccggcaa aaaccatctg
     2581 gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat ccgtattgat catcaccgtg
     2641 gccgatatta tgtcggcgat gaaagatacc ttcaggaata gcagtaccag cgaagaacaa
     2701 ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg atgagatcgg cgtgcagacc
     2761 gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg atcgccgctc ttcttccaaa
     2821 cgcccaaccg ggatgctgac caacagtaat atggaagaga tgaccaagtt gctgggcgaa
     2881 cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg tgatcttcaa ctgggatagc
     2941 taccgtagcc gggtaacagg taaagagtat taatccccgt ttctggcggt atactgcgac
     3001 acaattccac cttgagtaat gatgatgaaa actgttaaac atcttctgtg ctgtgccatt
     3061 gccgccagcg cattaatttc caccggggtg catgctgcgt cctggaaaga tgcgctctcc
     3121 agcgccgcca gcgaacttgg caaccaaaac agcacgacac aggaaggcgg ttggtcgctc
     3181 gcgtcattaa ctaacttgct tagcagcggg aaccaggcct taagcgcaga taacatgaac
     3241 aatgccgcag gcattctgca atactgcgcg aagcaaaagc tggcgtcggt aaccgatgcc
     3301 gaaaacatca agaaccaggt gctggaaaag ctgggcctga acagtgaaga acaaaaagaa
     3361 gacaccaact atctggatgg cattcagggt ttgctgaaaa ccaaagatgg tcagcaactc
     3421 aatctggata acatcggaac gactccgctg gcagaaaagg tgaaaaccaa agcctgcgat
     3481 ctggtgttaa agcaggggct gaacttcatt tcctgaaccg cgaaacgccg cgttttagct
     3541 gttacccggc tgacgcggct ctctcctgcc cttttccctg ctcctggacg gttttccccc
     3601 tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta taaactaatt cctaacaaca
     3661 tcgcattttt ttgacactag aattgcagca atatggcgac gccattacat tgtgcttctc
     3721 aaaaagctta aacgttggct aaacggcccg gtttagtgag gatcgcccgt tgtcagaact
     3781 actctctttc gccctttttc tcgcctctgt gctgatttac gcatggaaag cgggacgtaa
     3841 cacctggtgg tttgcagcca cattaacggt gctggggcta tttgtcgttt taaatatcac
     3901 cctgtttgcc agcgactatt ttactggcga tggtattaac gacgcggttc tctatacctt
     3961 aaccaatagc ctgaccggtg ctggcgtcag caaatacatc ctgccgggta tcggcattgt
     4021 gctggggctg acggcggtgt tcggtgcgct gggctggatc ctgcgccgtc gtcgtcatca
     4081 tccgcaccat tttggttaca gcctgctggc gctcttactg gcgctgggtt cggtggacgc
     4141 cagcccggca tttcgtcaga taacggaact ggtgaaatcc cagtcacgcg acggcgaccc
     4201 ggactttgcg gcttattata aagagccgtc gaaaactatc cctgacccga aactcaacct
     4261 ggtttatatc tacggcgaaa gtctcgagcg aacctatttt gataacgaag ctttcccgga
     4321 tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg gatttcagcc acacgcagca
     4381 gctgccagga acggattaca cgattgcggg catggtggct tctcagtgcg gcataccgct
     4441 gtttgccccc tttgaaggca acgcctccgc ttctgtttcc agcttcttcc cgcagaacat
     4501 ctgtctgggc gatatcctga aaaactcggg ttatcagaac tatttcgtgc agggcgcgaa
     4561 tctgcgtttt gccggtaaag atgtgttcct gaagtcgcac ggtttcgacc atttatacgg
     4621 ctcagaagag ctgaaaagcg tggtggccga cccgcactat cgcaacgact ggggattcta
     4681 cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa gagctttccc gttctagtca
     4741 gcgattctca ctgtttaccc tgacagtcga tacccatcac ccggatggtt ttatctcgcg
     4801 tacctgtaac cgcaaaaaat atgattttga cggtaagccg aaccagtcat tcagcgcggt
     4861 aagttgcagc caggagaata tcgcggcgtt tatcaacaaa atcaaagcgt caccgtggtt
     4921 taaagatacc gtcatcgtcg tctcttctga ccatttagcg atgaacaaca cggcgtggaa
     4981 atacctcaat aagcaggatc gcaataacct gttttttgtc attcgtggcg acaagccgca
     5041 gcaagagacg ctggcagtga agcgtaacac gatggataac ggcgcgacgg tgctggacat
     5101 tctcggtggc gataactatc tcggacttgg tcgtagcagt ttatccgggc agtcaatgtc
     5161 ggaaatcttc ctcaatatca aagagaaaac attggcgtgg aagccggata tcatccgcct
     5221 gtggaaattc cctaaagaga tgaaagagtt caccatcgac cagcagaaaa acatgattgc
     5281 cttctcgggt agccatttcc gtttgccgct gctgttgcgg gtttcagaca aacgcgtgga
     5341 accgctgccg gaaagcgaat actcagcacc gctgcgtttc cagctggccg atttcgctcc
     5401 acgcgacaat ttcgtctggg ttgaccgttg ctataagatg gcacaactct gggctccgga
     5461 actggcactc tccaccgact ggtgtgtctc gcaagggcag cttggcggtc agcaaattgt
     5521 tcagcatgtt gacaaaacaa catggcaggg caaaacggca tttaaagata cggtcatcga
     5581 catggcgcgt tataaaggca atgtcgatac gctgaaaatt gttgataacg atattcgcta
     5641 caaagccgac agtttcatct tcaacgtcgc cggtgcgcca gaagaggtga aacagtttag
     5701 tgggatttcc cgtccagagt cgtggggccg ctggtccaac gcgcagctgg gcgatgaagt
     5761 aaaaatcgag tacaagcatc cgctgccgaa gaaatttgac ctggtgatta ccgccaaagc
     5821 atacggcaat aacgccagcc gtcctattcc ggtacgcgta ggcaatgaag aacaaactct
     5881 tgtgctgggc aatgaagtga ccaccaccac actgcatttc gataacccaa ccgatgccga
     5941 cacactggta attgtgccgc cggaacctgt ctcaaccaac gaagggaata tcctcggaca
     6001 ctcgccgcgt aagctcggga tcggcatggt ggaaattaaa gttgtggaac gtgaagggta
     6061 atttctgact gatgtgtagg tcgcataagg tgcgacctac acatcttctc cggcctacaa
     6121 aacatcattg agaaacagaa aaaaaaacag cgcccaacac ttccccggac gctgcatggc
     6181 aactttaata atctcgcctt atcagaaagt aattacaccc ttaatcaact cacgattgtt
     6241 aatcacatcg cgctcgtagg tttctgccag cgtggcgaac ggatagcgat gggttaacat
     6301 catgtcagca gtgatttttc cttccgccat cagtcgaccg actttagcaa aatcttccgg
     6361 cgtggcgttg cggctaccca tcatcgtcgt ttctttttta tggaattccg gatcggagaa
     6421 ctgcaactca cctttaaaca ggccgacaaa taccaccgtg ccgccgtgac gaatcaaatt
     6481 cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg atcactttct gcgccagcga
     6541 accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg gctgacgggt ccagcacggg
     6601 caactccaga cgcgttgcca catgttcacg gcgcgctgga ctggtatccg ccaccaccac
     6661 ctgtgcgcca tcagctttag cgattgccgc cgcgcccaga ccgattggcc ccgccccgac
     6721 caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc accgcatgag cgctaatagc
     6781 gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg tctgcgggca aaatgttcgc
     6841 caccggcacg ctcaaatact cactaaaacc gccatcctga tgcacgccaa tgactgaaat
     6901 tttttcacag caattggtac gcccgctttt acacgccggg cattgctgac aggcaacata
     6961 agggatcaca gcaacttgct gaccattttt aagatcagca atatttttac ccagcccaac
     7021 aatctcccca catatttcat ggcctaaaac acgtggataa ctaaaaaatg gttgatttcc
     7081 accccaggca tgaatatcgg taccgcaaat cccgacagac ttaattttta ttaatgcttc
     7141 attgtcaccc ggaataggta tctcgcgttg tttccagact aattctttcg gctgctggca
     7201 aattaaaaca ttcatcgtag acatgattcg gctctccatt ttttgttgcc ttagatatca
     7261 atgaaaatga aaaatggcag cggaacggaa aatctgtttt gtgaaaacac acgcataaaa
     7321 atgttttaaa tcgggtttta atggactcac aaggaggaaa atatgagtcg ttcacaaaat
     7381 ttacgccaca atgtgattaa ccaggtcatt gatgatatgg cgcggggtca tatcccttcc
     7441 ccacttccat cgcaaagtgc gctggcggaa atgtacaaca tcagccgcac tacagtgcgt
     7501 catattctca gccacttacg cgaatgcggc gtcttaacgc aggttggaaa cgactatgtg
     7561 attgcgcgta aacctgacca tgatgacggt ttcgcctgta ctaccgcttc aatgagtgaa
     7621 caaaacaaag tgtttgagca ggcttttttc acgatgataa accagcgcca gttacgccct
     7681 ggggaaactt tttctgaact gcaactggct cgggccgcgg gtgtcagccc ggttgtagta
     7741 agagaatatc ttttaaaatt cggccgttat aatcttattc aaagcgaaaa gcgcggccag
     7801 tggagcatga agcaattcga tcaatcctac gccgagcagc tgtttgaact acgcgaaatg
     7861 ctcgaaacac attcacttca gcactttctc aacctgcctg atcatgatcc ccgctggctg
     7921 caggcgaaaa ccatgctcga acgtcatcgg ctactgcgtg acaacatcgg caatagcttt
     7981 cgcatgttct cgcaacttga cagagatttt cactccttac tgctttcagc tgccgacaat
     8041 atcttttttg atcaatctct tgagatcatc tcagtcatct ttcattttca ctatcaatgg
     8101 gacgaaagcg atcttaaaca gcgcaatatc attgccgtcg atgagcatat gaccattctc
     8161 agcgctctga tttgccgcag tgaccttgat gccacccttg cgctccgtaa ccatttgaat
     8221 tcagctaaac aatcaatgat tcgatccatt aatgagaaca cgcgttatgc tcattaagta
     8281 cggattaaaa acagcaaact attagcgggt gcacggtttt atcaaaagat aaataccggg
     8341 aaattcccgc ttacctatgc tcacaatcag tcgactaaaa atgtttttgt gagcggtagt
     8401 aaagtcctaa aactttaacc tgccgttaat gtcaaccttg cgtaatgcac aattacgtaa
     8461 acaattaaaa ttataaatac aggagtccgg cgtggaaaaa gaaaatatca ccctcgatcc
     8521 gcgttcttca tttactccat cttcgtcggc agatattccc gtgccaccag atggattagt
     8581 tcaacgcagt acccgaatta aacgcattca aaccaccgcc atgttgttat tattttttgc
     8641 ggcggtaatc aattatctcg accgcagttc gctgtcggta acaaatttaa cgattcgtga
     8701 agaattggga ttaagtgcca ccgaaatcgg cgctttgctc tccgtgtttt cactcgctta
     8761 cgggattgcg caacttcctt gcggcccact attggatcgt aaaggcccac gcctgatgct
     8821 gggactgggg atgttcttct ggtcactgtt ccaggccatg tctggcatgg tgcacagctt
     8881 tacgcagttc gtgttggtgc gtatcggtat ggggattggt gaagcgccga tgaacccatg
     8941 tggcgtaaaa gtcattaacg actggttcaa catcaaagag cgcggacgcc cgatgggctt
     9001 cttcaacgca gcttctacca ttggcgttgc cgtaagccca ccgattctgg cggcgatgat
     9061 gctggtgatg ggctggcgtg ggatgtttat taccattggt gtgctgggga tttttctcgc
     9121 cattggctgg tatatgctct atcgcaaccg cgagcacgta gaactgactg ccgttgaaca
     9181 agcttatctc aatgcaggta gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg
     9241 gcgcagcctg ttccgtaacc gcacaatgtg gggaatgatg ctcggattca gtggcatcaa
     9301 ctacactgcg tggctgtatc tggcctggct tcctggttac ctgcaaacag cctataacct
     9361 ggatttaaaa agcacagggt tgatggcggc tatccctttc ctgtttgggg ctgccgggat
     9421 gctggtcaac ggttacgtta ctgactggct ggtcaaaggg ggaatggctc cgattaaaag
     9481 ccgtaagatc tgcattattg ccgggatgtt ctgttctgcc gcctttacgc tggtcgtacc
     9541 gcaagcgaca acatccatga cggcggttct gctgattggc atggcactgt tctgtattca
     9601 ctttgccgga acatcctgct ggggcttgat ccacgtcgca gttgcttctc gcatgacggc
     9661 gtcggtgggc agtatccaga actttgccag cttcatctgc gcctcttttg cgccgatcat
     9721 tactggtttt attgttgata ccacccactc attccgtctg gcactaatca tctgcggttg
     9781 cgtcaccgca gcgggagcac tggcgtacat cttcctggtt cgtcagccga tcaacgaccc
     9841 gcgtaaagat taaactccct atgcccgata acattttgct tatcgggcat ttttatggcg
     9901 attaaaacgt ttcccagttc tccccgctat ctgccactgc cattttacgc ggcgtagctg
     9961 gcgtcacgtt ttttaccaca gccgatgttt cacgctgctg ttgctgctgt tgctgaatcc
    10021 ggaacactgc cacagcttcg gtcaggcgac tggcctgctc ttccagcgcg gcggcggcag
    10081 cggcagattc ttccaccagc gcagcgttct gttgagttac ccggtccatc tcagcaaccg
    10141 ctaagccaac ctgatcgata ccacggctct gctcatcaga agcagaagca atttcgccca
    10201 taatgtccgt cacgcgggtc acggcactga caatctccgc cattgtttcc cctgcgcttt
    10261 cgaccagcgt agagccaaca tctactttcc ccaccgagtc ttcaatcagg cttttaattt
    10321 cacgagccgc ctgagcgctg cgctgagcca gattacgcac ttcacccgcg accaccgcaa
    10381 aaccgcgccc ttgctcaccc gcgcgcgctg cttcaaccgc cgcgttcaaa gccagaatat
    10441 tggtctggaa ggcaatgccg tcaattacgc tgataatatc ggcgattttc tgcgaactgg
    10501 tggagatatc gcgcatggtc tgtactacgt tatccaccac tttgccgccg cgttgcgccg
    10561 tttcagaagc acttaacgcc agatggctgg cctgacgcgc attctcggcg ttctgtttca
    10621 ccgttgcggt cagttgctcc atgctggctg ccgtctcttc cagcgaagcg gcctgttgct
    10681 cggtgcgcga agagagatcg ttattgccgg tggcgatttc gctggcaccg ctatagatgg
    10741 cattggcccc gttgcgcaca tcaccgacgg tacgcatcag ctctccctgc atatggcgca
    10801 aactctctgc cagttgcccc atctcattag agccatccac ctcaatcggt ttcaccagat
    10861 cgccgcctgc aatatgacga atgctgtcaa tcaggcgatt cattggcgct accagcgagg
    10921 ctttaatacc gaaccagacg gcgaagatga ccgccagtac gacgatcatc acgcccacca
    10981 gaatccacat cgcctggctg taggaggcat tgttatcgct gacggcgata tcatagagcc
    11041 gatcgtcttg ctccatgtaa gctacatact gcttctcgaa accgtcctga tatccctggg
    11101 tcggctgatc aaagaactcg ttgattttgc ctgcacctaa tagttggatc agctccgcca
    11161 gcgcattgtg ataaatatcg taattacgtt tgatctctgc cgctgcggcg gtgcactgac
    11221 gcgggtcacg cggcaacgct tcgtaatccg cccagttttt ttccgcctgt ttcagcgaaa
    11281 tactggcact ctgcatcagc tcagcaacgg ttgaaccgct accaatatta ttctgatcca
    11341 tcatgtagcg gatacccgcg cggttgaggg tgttacgcgt ctgcaacaac gcgacccagc
    11401 tgccattcag cgtggattgc tgctggcgaa tggtttgtaa aacagtgaaa ttttctttgt
    11461 cattctttaa ggcattaaag aacagaccgc ctgatgtcag ttgtaaaagg ccaaaaacag
    11521 ccaaaaccag cagtaagctg gtcacaattt tgatacgttt taacatagtt tctctttcct
    11581 gtggaccgat gcaagatttt cggcctggaa aggaaaaact ttatgaatat cccgcggaga
    11641 ttacgccgag taattttatt cacactctga atttaaaaag cca
//
LOCUS       DAZFYC010000146        11439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFYC010000146 DAZFYC010000000
VERSION     DAZFYC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.146"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..866
                     /locus_tag="ACHKW5_004418"
     CDS             <1..866
                     /locus_tag="ACHKW5_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO7513665.1"
                     /translation="TTATPTTAVSSQSNALPPRPLLQATQLPALVMTGKPGQSSVVYL
                     REARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQL
                     YGHMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQALVPPAVS
                     ASRVQSTTGTSGTSAPRTTTPAPVVTKPVAPSSQKVTPPRQWKMEKGTTLKDGLARWT
                     SSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEKPLYARTSTAQ
                     CLLRVTDRPQE"
     gene            871..1308
                     /gene="pilM"
                     /locus_tag="ACHKW5_004419"
     CDS             871..1308
                     /gene="pilM"
                     /locus_tag="ACHKW5_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO7513666.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSITLPSVIPDHVILWMN"
     gene            1339..2958
                     /locus_tag="ACHKW5_004420"
     CDS             1339..2958
                     /locus_tag="ACHKW5_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO7513667.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLAYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTPLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2979..4274
                     /gene="pilO2"
                     /locus_tag="ACHKW5_004421"
     CDS             2979..4274
                     /gene="pilO2"
                     /locus_tag="ACHKW5_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO7513668.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPRYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSVTDENGQIIQPPWKEYDLQIQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4264..4716
                     /gene="pilP"
                     /locus_tag="ACHKW5_004422"
     CDS             4264..4716
                     /gene="pilP"
                     /locus_tag="ACHKW5_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO7513669.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGGPAEAGRQQESISAVTTNTQASLSPRLTAIFGVHPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            4801..6309
                     /locus_tag="ACHKW5_004423"
     CDS             4801..6309
                     /locus_tag="ACHKW5_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO7513670.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARIREATTIAGQENGMNPVQNKMLGYIRHAATLNAS
                     DLHITPGRDNTDFTYVEARVHGELEILDIIRKDEGVELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAEKFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSSHIPTFGMLGYHPKQE
                     QTIRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQHGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERRLVETFCNADAVYLRNHDGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            6311..7411
                     /locus_tag="ACHKW5_004424"
     CDS             6311..7411
                     /locus_tag="ACHKW5_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO7513671.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRTEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            7485..8021
                     /locus_tag="ACHKW5_004425"
     CDS             7485..8021
                     /locus_tag="ACHKW5_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO7513672.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            8075..8548
                     /locus_tag="ACHKW5_004426"
     CDS             8075..8548
                     /locus_tag="ACHKW5_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO7513673.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            8567..9202
                     /locus_tag="ACHKW5_004427"
     CDS             8567..9202
                     /locus_tag="ACHKW5_004427"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO7513674.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGYYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAATGTMVCMSLFPRSNTRAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            9207..10571
                     /gene="pilV"
                     /locus_tag="ACHKW5_004428"
     CDS             9207..10571
                     /gene="pilV"
                     /locus_tag="ACHKW5_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO7513675.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHRGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVIMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWVRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCAPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            10829..10987
                     /locus_tag="ACHKW5_004429"
                     /pseudo
     CDS             10829..10987
                     /locus_tag="ACHKW5_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            11029..11319
                     /locus_tag="ACHKW5_004430"
     CDS             11029..11319
                     /locus_tag="ACHKW5_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513676.1"
                     /translation="MKHLSPRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT         2939 a         3137 c         2838 g         2525 t
ORIGIN      
        1 caacaacagc gacgcccaca accgcagtca gcagccagag taacgcactg cctccccgcc
       61 cgctccttca ggccacacag ctgcccgcac tggttatgac cggtaaaccc ggtcagtcat
      121 ccgtcgttta tttacgtgag gcacgtcagc gctccgtcag tgactggata cgccagttgc
      181 tgccacccgg ctggcagttc cggcaacaga acgcagccat accggcactg aacaaacgcc
      241 tccccgtctg gtccgttaat gaccagtggc accgggcact gaatactctg ctgaccgggc
      301 agcaactgta cggacatatg gactggaaca ggaaaatact gactgtcacg accactgcag
      361 caccgcctgt tgacctcacg gcaccgcagg gttcacagaa aacagcggac acaccacgta
      421 atccgttcag gagcacaacc ggaacggctg cagaccaggc actggtacca cctgccgtca
      481 gcgcttcccg ggttcagagc acaacaggga catccggtac atctgcgccc cgcacaacca
      541 cgccagcccc ggtggtcacg aaaccagtag caccatcatc acagaaggtc acaccgccac
      601 ggcagtggaa aatggaaaaa gggaccacgc tgaaggacgg actggcccgc tggacgtcgt
      661 ccgaaaaatg cccccacggc acctggacgc tgcgctggga aacacccgtt aactacagta
      721 tcgatgcccc tctggttttc accggcagtt tccgccaggc gctggaacag ctcttcgcac
      781 tctacacccc ggtggaaaaa ccgctgtatg cccggaccag cactgcacaa tgtctgctgc
      841 gggtgacaga tcgtccacag gaataacctt atgggctggt tcatcgtgtt catcatggtc
      901 atcacgacca caggcaattt ctggttcacc agtcagctct cccgcagcga acaccgtcat
      961 caggccgcag aaaacacaca acaggcagca acgttcattc gttatatgaa cgccatcaat
     1021 gattacctgc atcagcatcc ggaacgacgg acagccggag ggcggctgac atccgctcag
     1081 ctcgggattc ccgggacgga taccgtcagt catatcatca gccagcaacg tgtttttgtc
     1141 tgggctacag aaaccccggg cctgatggcc gcgctcaggg aacagagtaa tgactctgct
     1201 ctgctcggca gggtggaaaa cggccgactg ctggacactg ccggcagagc gctttccatc
     1261 acacttccgt cagtgatccc cgatcacgtc atcctctgga tgaattagtc acgaatctga
     1321 ataaatcacg gatctcccat gaataaaaaa attcttgcca ttatgattgc cgccgctctg
     1381 ttacagggct gtgccagtta cagacagctt gagagcacaa gaaaggaagc cgttgcggaa
     1441 caacaacggg cacgggaaat gatgtctgat ctgcgaaccc ggacgccggt tgttaaggaa
     1501 tcctcacgac aatggatcaa tccccgtccg gtcatggata aaaacaccgg caaaaccgtt
     1561 cccggttgtc ccattgtcat tcatacccgt cagagcatca cccttcagca ggtggttcag
     1621 cggatcacgg aaagctgcca tattccggta cggatcacac ctgatgtact ggcatacctg
     1681 aatacatcca caaccggcag cacacaacaa atccagggcg cgttaccccc accggatgcg
     1741 agcggcatgg ttccccttgc cgccattggc gcatccgaca cgccaccatc ccgtttgccg
     1801 ggaaataccg cagcactgtc tgaactgaat gcctccggcc tgacgcccct gctgaatgcg
     1861 gtctcctccc gtctcggtat atcctggcgc tatgacggct cgcatatcat cttctattat
     1921 ctcgagacac agaccttccc catcacctat atggacagtc aggtggccta taacgccact
     1981 gtggtcagcg gcaccatgtc ctcaaacggc tcatcaggag gcagtgcatc agatgccatt
     2041 tccggtgatg ccagcaatac ccagaccacc accgtggata tgaagtctgc gctgtatcag
     2101 gatgtgaaaa atgcggtcac atcaatgctg accccgggta tcggacgcat gttcatgtcc
     2161 acgggcttta tcaccgtcac agataatcca caggttctgg aaacggtccg ggcattcatt
     2221 gaaaaacgca atgaagagat gaaacgtcag gtggtgctga atgtggaaat tctgagtatc
     2281 cggaaaaccc gcaatgagca ggccggtatc gactggaacg cagtcttcag cgacagaacg
     2341 ctgggactga gtctcggcag cacattcacc agtgcagcca gtgatgccat cacaggtggt
     2401 gtttccattg tggacggtaa actcaccggt tcaaaagcct tcctgaaagc attgtcttcc
     2461 cagggggatg tcagtgtggt gacccagaac tctgccgtca ccaaaaacct gacgccggtg
     2521 ccaatgcaga tcgccaatca gcaaagttac atcgagagtg tgacgacgga taccacagcc
     2581 aatgtcggct catccacctc cctgaatgcg gccaccatca ccaccggttt taacatgacc
     2641 cttctgccgt tcattctccc ggactcacag acactgcagt tgctctattc catgagtctc
     2701 tccgacaaac cggttattga gaattatgag agcggtggaa gtaaagccca gctgccgaat
     2761 gtggatctga aaaccatcaa tcagaccgtg gatctgaaat ccgggcagac ggtcattatc
     2821 agtggcttcc agcagtcagg acgtcgcagc ggaaaacagg gtgtaggcac gccgggattc
     2881 ttcggtcttg gcggtgggat caacagtgaa aacgacgaca ccattctcgt ggtgctgatc
     2941 acgcccaata tcatctgatt tgcacaagga ggacgatcgt gacggatatt tcaccgacag
     3001 ggcggcaggt gtttccgctg acggcagggc gcaatgtgtt tctggccggt ctggaatgga
     3061 aaacactgcc ctcacagtac cggcatgccc gcgactttgc ccgggcacag aaagccgacc
     3121 ttttcctggc ctgtcagtat ctcagtaatg aagatgccga tacccacacc atggtagcca
     3181 ctgtcagtcg ccgcatactg cccggaaaac cccgtcagtg cttctccctc gccctgctga
     3241 tcctgccact acttgaacat ggcggctatg ccatcacgga actgacgtta cccggagaaa
     3301 caccgcgcta tagctttgtt tccgttgtcg acggtgttct cgtcagtgac ctggtgggaa
     3361 gcggggaaga ggttcgggaa gcccgggata cctttctttc tatcaacacc gaaccgactc
     3421 agggctggat acactatgaa ccagcggcat tttcagcagg cgctcagaat cagcacctgc
     3481 cgctggcctc cctgactggc tctggtaaac atccggctgc cgcacgcctg aatccggtat
     3541 cccggggacc gcagttcatc actgtctctc tgatcatcgc ccttctcggc actgcatggt
     3601 acggctggca gtattatgaa cgctggcaga cagaaaaagc cgcgcaggca gcggttgccc
     3661 gacaggccac agaggaacga atcactcccc cctggcccgg actccctgaa acagacgcgt
     3721 tcatccgggg atgtgctgat atatggactt cactgccact gtctgctgcc ggatggcgtt
     3781 attcgctggc agaatgctcg actgacggtg gcagcggcaa tcttcgcgca tcgtacacca
     3841 atactgcggg cgcgacagtg actgacttca tccggcgcat cagtgatatg acagacagcc
     3901 gaccgtttat tgtgatgccg gaaggaaaga ccggcggcat tggcctgccg gtcaccttcc
     3961 ggctgtctga aaacgccgtc acagttgatg cactaccgga aaccgctgtc ctgcaggaac
     4021 gactgacctc gctggctcag tcaatgcaac tgaaactgga ctggcaggaa gttaataaca
     4081 gcgtcactga tgagaacgga cagatcattc agcccccatg gaaagagtat gaccttcaga
     4141 tacagaccac actccctgca caccatctgg ctgaacgcct gaaagagcct tctgtccgtt
     4201 ttatctctgt tacacggcaa ctggagaacg gtcgtttcca ctatcaattt acaggtaaat
     4261 attatgcccg ataaagccaa aacccttctg ttttcagtcc tgctgttacc ggccacggca
     4321 atggccacaa ccggaaatgc agagcaacag aacacacagc aaataaggat tgaacagata
     4381 caggaagaga cagccctgta tgaagccatg ctggcaagag agaagatccg ccatgaactg
     4441 cgaacactgg agcagggggg gcccgcagag gccggacgtc agcaggaaag catttccgca
     4501 gtcacaacaa atacacaggc atcactatcc ccccgcctga cagcaatatt cggtgttcat
     4561 cctcagctga ctgcccggat agaacttccg gggggaagca tctccgatgt gaaggcgggc
     4621 cagaacatcc ccggaaccac ttatcacgtg gaaaaaatca cgccggacag tgtggtgctg
     4681 gaacagtctg gcaccagaaa aaccctgcga ccatgagacc ggccgccgta actggcggct
     4741 gtcaaaaata aaaggatgaa agacatattt tccgggcacc gctgcccgta aggaggcttt
     4801 atgctggatc cagcagacct gccgccggat gacattctgg attatgtggc catcgacacc
     4861 gataacgccg gacaactgcg gatcagggtt gttgagggaa aaaaacatct gcgtgccgtc
     4921 caggaatatc tgacccgcct gcgcgcacgc tatcagggac gagtcggaga ctttgaattc
     4981 accacactgg atgtgatagc ccggatccgg gaagcaacaa caatcgccgg acaggaaaac
     5041 ggcatgaatc ctgtgcagaa taaaatgctc gggtatatcc gacatgccgc cacccttaat
     5101 gcctccgatc ttcatatcac tccgggccgg gacaacacag acttcaccta tgtggaggcc
     5161 cgtgtacatg gggaactgga aatcctcgac attatccgga aagatgaggg agtggagctt
     5221 ctgggggcca cctactccgg gatgactgat gtcatcaagg gaacacaatt tgatcctggt
     5281 gttccccagg acgcccgact ggcggaaaag tttctgaaac ttgcggggtt gtttggcgca
     5341 cgttactccc attatccctg cgtggggggc ctttatgctg ttctgaggct gatcaaggac
     5401 gacagcagcc atattccgac cttcggtatg ctgggctatc atccaaagca ggagcagacc
     5461 atccgccgta ttctgcaacg accggaaggt atcatcaccc tgtcagggcc gaccggctcc
     5521 ggaaaatcca ccacgctgag gacggcgtct gcggcctatc ttgaacagca cggattcaac
     5581 aacaccggtg gtattctttt gccccgtcgt cgtctcttca ccattgaatc accgccggaa
     5641 ggacgtattc ccggcgcaat ccagaccgcc gtcatggaca gtgctcaggg ctgggtggat
     5701 tccataaaat cagccctgcg gcttgatcct gacggcattc tgaacggtga aatccgcgat
     5761 cacgattccg ccattacggc aataaaagcc gccatgacag gccatctgat gctgaccacc
     5821 atccatgcga acgacccgat taacatcctc gaacgccttg agatggaagg tgttcaggcc
     5881 aggatgattg cagacccgca gttgttcatc ggactgctca gtcagcgtct ggttcagctg
     5941 atatgtcccc actgccgtca tccatggaat gacgtggcat cccgtcgtac tgaggatgaa
     6001 cgacgtctgg tggaaacctt ctgtaatgct gatgcggtat atctgagaaa ccatgacggc
     6061 tgcccacact gctggcgtgg tgtcacaggg cgcacagtga ttgcagaagt catctctcct
     6121 gacgccagat tttttcagtt ataccgggaa aaaggccgta tcgaagccaa aacctactgg
     6181 cacagggaac tgggcgggat gacgcgtaat cagcatctgc tgggaaaaat caacagcgga
     6241 ctggttgacc ctctggcggc ccattacatc tctcccattg atgaggacag ctatacgctg
     6301 ctgcgctgat atgaaatttc tggaaaaact caacagtact gaactggatc tgtctgccct
     6361 gaacagctgg acagccagaa aaaccttcgg tcttaagcgt gctgagttct atgagtccct
     6421 cggcggaatg atacgtgacg gaacgccgtc actgcgcgcc ctggagttta tctgtaatgt
     6481 cgaaactgac tttggcaccc ggaaaggaac atccggcatg tactttctgg ccagagaatg
     6541 tattgcctcc ctgaaagcca gcggtatgct gtcgggggcc ctgcctacat gggttccccc
     6601 ggaagaagca gcactgatca ggaacggtga agaacgcggg aacatcgccg aagccctgtt
     6661 ccaggtcgca aaaaccgtga aagcacggcg ggaaatggtg agcagtctca tcacactctg
     6721 tctttatccc cttctgctgc tttcactatg tgtggtgaat atgtacaacg cacatacccg
     6781 catcatcccc attgtcagcg cttttgcacc ggaggagaaa tggtcattcc agatgaaagt
     6841 ccttgcagcc atgtcagagc ttgtcatcgg ctatggtctg tggctcagtg gtgccatcat
     6901 cacgcttgtc atcatcatcc gcttctcact cagtcgctat accggcccgg gacgcaggct
     6961 tcttgacaaa ctgccaccat ggtcgctcta tcagactttt catggtgtga attatctgtt
     7021 cagcatatcg tcaatgctgc agataaatat tccgctttca caggcgctgc tcagaactga
     7081 gaaaagtgcg gagcataata aatggttaaa ggagagaatt caggcaataa gaaaaaatgt
     7141 attatccggt cagaatctgg ccaccgccat gcgcaattct gactatgatt ttccttcaaa
     7201 aatatgcatc aataaacttc tcttaaattc tgaaagggaa gacagcgtat ctgcaatgaa
     7261 tacctatgcc ggacagtggc tggaagatgc aaaaaagagc gtcagaaaaa gtggattaat
     7321 tatcaccggt atttcaggag gttttgtatt cttttttatt cttaacatga ttagcgcgat
     7381 atacagtatc agcgatatgc tcaagcaata atatctgccg taacactaaa acgcccttca
     7441 atatactttc attaacgtaa taaccattaa tacaggagtc atatatgaac ttcagagcac
     7501 gtctgtcttc ttatttttct ttaaaaaaag ataaagaaga tgataaaggt ttaacattat
     7561 tagaaattgc cggcgcaatg attatcatcg caatagttgt cgcgctcgcg atgagccaga
     7621 tgtccagcac aatggcctcc agtgacaacc agcgcgaact gaaaaacctg cagacaattg
     7681 caaccaaaat gaagcaatac aagttccagg gaagatatac cggaacggat tatatcaaaa
     7741 ccctgacgga aagcggtgca ttgcctgctg atatgattgc aggaggcaac aaagccaaaa
     7801 acgcatgggg cggtgatgtc acgattgctg ccacagcaga caaatatggc tacattatca
     7861 ccagtaataa tgttcctaaa acaaactgtg ttgagctgat taacagcctg cgcagttcgt
     7921 ccatatttac caaaattatg aacacagccc cgaacacggt ggatcccgtt accgtctgca
     7981 gtagcgataa aaacaatatc aagctggaaa ccaacagcta atgccctgcc acacagcact
     8041 ccctccgggg tgctgtttcc cggagcacat aatcatgaaa tggttttatc tctcatctct
     8101 tttcttattc tgtctcccgg cgactgccgc cgtaaacagt aacacactgg atagctgctt
     8161 tattcaggca ggaaaacgtt actccatcga tcccggacta ctgaaagtaa ttgcccgtaa
     8221 agaaagcagc ctgaatcccc gggccatcaa tcataacaga aatgccagcg gaaaaattat
     8281 cagcacagat tatggcctga tgcagattaa ttccacacat attcccggac tgaaaagaat
     8341 gggaattatc cgaagtgaaa atgaactgct cagtaatgtt tgcctgaata tacagacagg
     8401 tgcatggatc cttgcccggc attttcagca gtgtggtgtg aactgggagt gtctcggtag
     8461 ctacaacgcc ggatttaata aaaataacac tcaccgcaga atgaaatatg cccggctgat
     8521 ttatgccgca tatatgcagg gacgttaata acggagaata cgagccatgt tcctcatcat
     8581 cttactcgtg atctccgcta cgctgaccat acagttactt gccatcagac tggccggata
     8641 ttatctcatg caggtgcata ccctccggcg tccacgtcac ctctccctgc tctgtgctgt
     8701 tctggcactc accgccacca ttacgcacat catccggggg acagacatta ccggactttg
     8761 ctttctgttg ctgatggccg gctgcatgac actgatgaca ctgactgatg cagcctcctg
     8821 ccgtcttccc cgtcccttca ccctcacatt cctgatgctg ggtgtggcgt ttcgtttatg
     8881 gcagggcgat gctgctattg cgcttgcctg tgctctgttc tggtttctcc tgttatctct
     8941 cctgaggcgc tacacctcat accgcaaggg agtggagacg ctgggactgg gggacgtgtt
     9001 tctgattgca ggtgtggcca tgtggtcatc tgccaacagc actccgtgga tcatcggaat
     9061 tgccgccacc ggcaccatgg tctgtatgtc gctgttcccg cgcagtaata caagggccgc
     9121 tatcccgttc ggtccgttta tctgtgcatc actgtacatt ttcacttttt ttcctggtgc
     9181 catttttcac accggagggt aacaccatgc aaaaagacga cgataaaggt tttgttctgt
     9241 ttgaaattct ggccggactg attgtgatcg gtatcgccac accaatgatt tattcagaaa
     9301 ttgaaaactg gctgaatgag cagctttacc agagtgctgc atatcatgca gatgcctaca
     9361 ataccgcagc cagaaactat attgccgaca acaatgcccg tctgcataga ggcagccttc
     9421 cggcaaattt cacggctgat gatctgattc gtcagggata tctgaaacag gggtttaacc
     9481 attctccctt tggtcagagt tatatcaccg gtatccgccg taaccagacc accgggcgtc
     9541 tggaagccct gacctgctca actggagggc agacaatcaa agaagaaggg ctgcgtagtg
     9601 tggcgggtca gttgccgggg ctgggtggat ttatcagcaa aaatggaacg gcaacaggcg
     9661 cattcggagc ctggacagat aaaccgggag actacggact tacctgttcg acaggtcaca
     9721 tcgccgtcat catgagcggt gatgacctgc aggaaagtga ccgtctctac cgttttcagg
     9781 tagcaggtcg tcctgaactg aatcagatgc ataccgcaat caatatgggc gggaataaca
     9841 taaataacac cggcaatatc aatggtcaga gtgccacact gaaaggtgat attacgagtg
     9901 aagacggctg gctaataacc agaaacaata agggatggat gaatatcaca catggtggtg
     9961 gattcaccat gacagacagc cagtgggtac gcgcggttaa tggcaaaggc atcacaacca
    10021 ccggggaaat caaaggcgga aaagtcagtg gtggtacagt ccggtctgac ggtcgtcttt
    10081 caaccgggga gtatttacag cttgataaaa cagctacggc aggcacaaag tgtgcaccgg
    10141 acggacttgt aggccgcacc agtacagggg ccatactttc gtgccaatcc ggtatgtggg
    10201 caggttttga atcctaccct gtcggaagcc ctataccgtg gccatcagcc acccctccac
    10261 aaggctacct ggtgatgaac gggcagtcat tttcatgttc ccggtatcct cagcttgccc
    10321 gtgcttatcc cgggtgtaag ctgccggatt tgcgcggtgt ttttatccgt ggctgggaca
    10381 acggacgtgg gctggatgga gatcggaaca gacatctcct gagttatcag gccgatcaat
    10441 caggtgtcta tcatgagcgt gggggatggc tgaaagggca tcacagcggt atgccttact
    10501 gggcccaggg aagcacaacg gaaatgcgcc ccaaaaacat tgcctttaac tacatcgtca
    10561 aagcaagctg atgcatttaa ggcgtattaa cttttaactt acagcaacgt attttgaccc
    10621 agatcatgct tcccgaatac cccagtttac ctctgtacgc tatgaatcct gccgcaccat
    10681 agcctgcttt gccgtcacag acggcaaagc cgtttgagcc actacccctg ttcgcataaa
    10741 ccggttttct ggagtcttct tctgcgtcat aacaggccga ataatcaaaa accagtgcat
    10801 cgctgatatt attcttccag acaccggatt gttgctgcgt gcactggccc aggctgctca
    10861 gcggggtgaa cgtgtcccca caccaggcga gccacccgca aacattgatg aacgggtcat
    10921 gatttgtccg ttaacaaaca gccagttcat gacaaaagcg aacagtattt tcatatcctc
    10981 ctcctgaaca aataattttt gttcaaaatt aacgctatga gaccctttat gaaacactta
    11041 tccccccgtg cagccactgc ggataataat cgtatcgtta caattgaaga cgtttacaca
    11101 atcgcattct cgcaaccaat tcacaccgaa tcagcgttgg ggcaaaggtg gatgttgatc
    11161 catactggtg aatatgaaag tgccagcgat gcaaaacgct tccttgatac gctcgtcacc
    11221 gggcactgtg cttgcatggt aacaccatca cctgttctgg atcatgacac ctggctgcgt
    11281 atccgcctgc tgatgggcga gataagggat gccaaataaa cattcatggc cccaacacat
    11341 aaaaataacg ccggggactc tggcagaggt accattcttt ttacatagag ccaatatata
    11401 aacaactttt ctatgataat atctttatca tgttataaa
//
LOCUS       DAZFYC010000147        11394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFYC010000147 DAZFYC010000000
VERSION     DAZFYC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.147"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..177)
                     /locus_tag="ACHKW5_004431"
     CDS             complement(<1..177)
                     /locus_tag="ACHKW5_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO7513677.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPV"
     gene            complement(334..636)
                     /locus_tag="ACHKW5_004432"
                     /pseudo
     CDS             complement(334..636)
                     /locus_tag="ACHKW5_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(786..1028)
                     /locus_tag="ACHKW5_004433"
     CDS             complement(786..1028)
                     /locus_tag="ACHKW5_004433"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO7513678.1"
                     /translation="MSAEKFSVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(1109..2803)
                     /locus_tag="ACHKW5_004434"
     CDS             complement(1109..2803)
                     /locus_tag="ACHKW5_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7513679.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQQVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            complement(2830..3451)
                     /locus_tag="ACHKW5_004435"
                     /pseudo
     CDS             complement(2830..3451)
                     /locus_tag="ACHKW5_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
     gene            complement(3718..4383)
                     /locus_tag="ACHKW5_004436"
     CDS             complement(3718..4383)
                     /locus_tag="ACHKW5_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO7513680.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATSSDIPVANTLKSLLESGNNAALYDLYRFRSALAAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(4524..5165)
                     /locus_tag="ACHKW5_004437"
     CDS             complement(4524..5165)
                     /locus_tag="ACHKW5_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO7513681.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            5868..6740
                     /gene="repA"
                     /locus_tag="ACHKW5_004438"
     CDS             5868..6740
                     /gene="repA"
                     /locus_tag="ACHKW5_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7513682.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            7435..7626
                     /locus_tag="ACHKW5_004439"
     CDS             7435..7626
                     /locus_tag="ACHKW5_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513683.1"
                     /translation="MAENGYGLAGLGMGKVKSINQYRLTPGFGGFTLVPYVATSCRLP
                     CRLRGIRTIKPALNVFAKV"
     gene            7699..7950
                     /locus_tag="ACHKW5_004440"
     CDS             7699..7950
                     /locus_tag="ACHKW5_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7513684.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            7947..8234
                     /locus_tag="ACHKW5_004441"
     CDS             7947..8234
                     /locus_tag="ACHKW5_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7513685.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLHASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            8338..8928
                     /locus_tag="ACHKW5_004442"
     CDS             8338..8928
                     /locus_tag="ACHKW5_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO7513686.1"
                     /translation="MMKNPLSYFHIATFLLSGKSRNLSEYGKAEVIMRDKFVLHRLIR
                     NGMQRQRGFLMWWRLANEMFIYGNKKQRKCAIKIKNALMERYGCDIGLGARIGKGLVL
                     PHHAGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIGAHTCIIGLNVK
                     IGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            9031..9684
                     /locus_tag="ACHKW5_004443"
     CDS             9031..9684
                     /locus_tag="ACHKW5_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7513687.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEEG
                     GYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGLD
                     GKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSTNCHTGQQFEGRLHS"
     gene            9752..10066
                     /locus_tag="ACHKW5_004444"
     CDS             9752..10066
                     /locus_tag="ACHKW5_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513688.1"
                     /translation="MITVIISEVGGWREWKHRARTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVEITGHIWKPMWNRGICWNVKGPPVIITLIQGAAWNSENK
                     PR"
     gene            10502..10726
                     /locus_tag="ACHKW5_004445"
     CDS             10502..10726
                     /locus_tag="ACHKW5_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7513689.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            10723..11109
                     /locus_tag="ACHKW5_004446"
     CDS             10723..11109
                     /locus_tag="ACHKW5_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO7513690.1"
                     /translation="MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGVHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
BASE COUNT         2977 a         2605 c         2872 g         2940 t
ORIGIN      
        1 caccggtggc ggaagcagac gtttttgcgt atgggcgata atggcagcgc tttcgcgaat
       61 ttgtccgtcg acgaacacca gagccggagg tgtggtgtca ggaacaggcg tggcattaaa
      121 tgccgtacag ccgctgagta atgacggcag aaggagtgta agtgattttt tttgcataga
      181 ataaaatccg gtatattacg ggttacggta taagacaggt ggcgggtaac tggccttcag
      241 atgaatatca tttcatcggg ttattgataa tgaacgttca tgttcgtggg gggattataa
      301 gagtgaattg catttctgaa cgggagcatt attttatttc ttgcctgtta ttgaaacatt
      361 tccggttatg atctccgaat ggcaggtatc aataaaatca cttttccccg attatgtgtc
      421 aaagtcaacg tgggtgaacc tgtgtacatc ctgccatatg gcgaaacaga caggacaggt
      481 aacaggtagt ttgacttcag ctattttttc gcagctttca accagttgct gcgccttgtt
      541 gtatctgacg ttatagccat ttgtgcaaag cgtttcatac ccatgagctc ctgtaactct
      601 atgtgtggcg attctttcgt ggactctccc attaattaat aattattcac gcgttgtgat
      661 tgttctcatg cctgtttctc tgttggtcat agcataaaat aatctgcatt cctgaaaaat
      721 atcgcggttg tgaaatattt aatttttatc ccccttttat gagggataaa aagcaggctg
      781 tcaggctata actgaacgcg gtttctggat tttggtgcta atgtcacatc tttccattca
      841 cagctgcttc cgggtatacg ctctttgcgt cgtattatac gggagttctg acacacaata
      901 attaagccat tgtctgccgt ctgaaatggc attatagcga tgtttatatc ttctgttgca
      961 ggaatataac tgactttctt ttcacatgta ttggtgtata tgacaacgct gaatttttct
     1021 gcggacattt tcaatcctct tttaaaccag aatatcccgt gagggcattc tttgtgaaaa
     1081 agatgccgga aaaattccgg cacggggatt aaacctgttg tggttctgag ttatccggag
     1141 cggttaccgg ttcgctgaca tccgggacct tttccggggc tggcatgacc agtggacgtg
     1201 tcagggctgt aagtccttca ccggataacc ccacctcctt cagcagggag tcgatgagcg
     1261 gagcctgtgt acggtaagcc agagcggcgg ttaccgcctg ttcagcgaga tttccgccac
     1321 ttatgtcggt tgttgctgtt ttaccgcggg taacaccgcc attcagacca tccacctgaa
     1381 tgattttaat gccgtcaatg gatttcattg gttctgtgga cttctctatg acagcaggta
     1441 atgcatccag cagggcaaga cggaagcgga ggctggtctg ctcatccgaa agcacgttaa
     1501 tggcatcgtt cagtgcgcgc tgtgcttctg cttctgccag acctttctga cgggtggcct
     1561 ccgccagttc aataattgct gctgcctgga gttcggctgc ttctttttca gcctgtgcgc
     1621 gaaccgttag ttcaaccgct ttcgtttcag cttcctgagc agcagcaatc agggcaacct
     1681 gtttggtacg gtcggcctcc gcagtctggc gggtggtttc cacatcctgc tgggcctgta
     1741 cggcttcagc cagtgcttta cttgcccggg cttctgcctg tgactgctct tccgattttg
     1801 ccgccacggc aatggctctg gcctggctgg cgatttcggt tgcctgctgt tgctcaattt
     1861 cccgtatggc cacttcccgt tcggcttcga cctggcgggt acgcactatc tgctgtcgtt
     1921 cgatttcggc ttcctctatc ttacgttcag ccagaattct cgcggattct gcttcacgac
     1981 gtcgttcagc ttcaatagca gcaatactgg cactctgctc tgccgtacgg gtttttacct
     2041 gttgttgttg ctccagagtc atgaaggctt cctgctgttc aatctccaga cgtcgggaga
     2101 gtgcatcccg gtttttttcc ctgatggcca cttcaacatc ctgctcaact tcgttacgtt
     2161 cgcggcgacg acgttctgtt tcctgggtca gaagggtcag gccttcagca tcaaaggcat
     2221 tgtcaggatt aaaatgttcc cgtgcagtct ggttgaaact tgtcagcgac acactttcaa
     2281 gctccagacc gtttttggat aagtcttccg caacggtgtt ctgcacaccc tgaacaaagt
     2341 tctcgcgggc atcctgtaaa tcctgcatgc tcattctggc cgccgtggcc cgcagggcat
     2401 caacaaattt atcctcgacc agactacgca gatcttcagg tgtgagcgtg cgttgtccca
     2461 gcgtctgggc tgcagtagat attccttcgg ctgatggttt tacccgaaga aaaaaggcaa
     2521 cggcaacatc aacacgcatg cggtcgcggg taatgagact ttcagccgct gcacgactga
     2581 cttccagctt cagggtgttc atgttgacgg gaattgtttc gtgaaaaatg ggcataacaa
     2641 ttgcaccgcc actcattacc actttttggc ctcccagacc tgtgcggaca aaggcctgct
     2701 ctgccgatgc ccgacggtac aggcgggcaa atatcagccc gataacgaac agggtgagga
     2761 ccagcgcaat aacggtaaac aaccatcccg gaagattaaa catagtgaga acctctgtat
     2821 atgtcgggat taaagaacat ccggccaggg attcagttca cccagccagc gggaaccgga
     2881 aagacggcag acaataatca ctttgtctcc tcgtctgaat tctttcccgg cctccggctc
     2941 cagcaacagg taatgaacct gtccaaagcg gtctgtgaag cgaccttcac agggggaacc
     3001 cgccactgcg tgataaccgg tgatggtggc catacacccg atgaatgcct cctcggtgag
     3061 cgccgttgtt tcatcacggg gtaaccaggg ggcaatcagt ctgccggtgc ggtgtacgag
     3121 aggacatgtc agcagcaggc aaagtggtgc aaggagaatg ttcggggcag gagcctgcag
     3181 cagatgaata ataagaccct gcaggagaat gccggatata ccaaaaaaaa actgccagca
     3241 tacagaggac gatcagcagg gggatccgtc cgatgttcag ccagtccagc gcctctcctg
     3301 tcagcccctc cggtaaatca ggtgtatcca tagctgaaga gagggagtgt ccgcagagca
     3361 gggcgataag ttcagcgata ccagtaagca gaacaaaagt cagggctgcc agatagggtg
     3421 cgttatacgc ggtaagtaac gtcagtgtca tttttcctcc tgtggcaggt cagtcaaaca
     3481 acattatggc gtgaagcttc cgcttcttta attgtcagaa gaagcatatc gtcagtcatc
     3541 agaagacaag gggatgtctg caggagggga tgataacaac agaagcctca cgccggaatg
     3601 cgctttatga caaccggaga ggacggataa taaaaaatgg gacatccaca tcagcaaacc
     3661 gcgatttgcg aaaaaaagtc agcggagagt ggctgccgtt gtggctgtcc cggtatttca
     3721 gtgtaacagg tgattgctgt gatctatgcc ccattcggct ttcggtaaac ctgaatgccc
     3781 cacatgaaga atgcgtgaca gcatgtttct gccgctgccg ttcaggctgc attcgcgtcc
     3841 gtgcgcgagt ataaaacctt catcccggaa tatctcttcc acttctgaag attcgaggga
     3901 tgccggatga attaatgaaa atgccaccgg tctgagcatt ctgcccgtag tggaaaaggt
     3961 gtctctggta ctgttcagct gcaggatatg acgtttacct gtttgcgcac gggaaagtgt
     4021 ctcactcagc agctggtcgt tccatgcgca cgtgccaatc tgccatccca gacttctgca
     4081 cgcggcaaga gccgagcgga agcggtacag gtcgtataat gctgcgttat tgccactttc
     4141 cagcagtgat ttcagggtgt ttgctaccgg tatgtctgaa ctggtggcca gcagttttcg
     4201 cagggtagtg aactcttcag ccattcctct gaactcgcgc atctgacggg cagtgtcaag
     4261 ccagttcagc aggtaacgcc ggcgacgccg ttcgttgttt atgttgtccg gggcggtgga
     4321 gagtgcttcg agcatattct ccagtaacag aatgacaggg ttttcctggg atagaattgt
     4381 cataaaggcg tcctgaaaaa atgcccgaca gccgctctcc gtacaccgta aggtgtcacc
     4441 ggtgccgggc attgtggaaa cagaagggaa aaaaatcccg gccataaagg ccgggaaaag
     4501 tcgtcaaatt aagacagcaa ttttcatgtc aacgctccgt catcagatac caggtgcagg
     4561 agcagggcga tcctcgactc cggcagaggc tgtgcatcaa gccggaggag gtactggtaa
     4621 cgcgcttttg tcagccagag ggtggaggca gcatccagct cctccctggc tgccggattg
     4681 agcgcaggat ccgggtacac tttcatcaaa gactgaacga tatcctccct gacggttttg
     4741 atttcaccgg ccattttcac gaagtccaga aaggcactgt gacggcgaag gtgagtgaag
     4801 ctgtggcgtt cggggtctgc tttcaggaaa aagtaaccgg gaaacacggc gtgtatatgt
     4861 cgacggtaac cgcagacttt atccgtgcgg cggacttttt tcagtatcag gggtgtccag
     4921 ggaaggacgt gttgttcgga aagccaggaa aagagatgtt cgcggttttt gccggcaggg
     4981 atgtactgag ccagatacca gttgcgttga tgtaattcac tgatattcaa aatccggaac
     5041 gctccgcgaa aatcgatccc cgcagagggg cttcgatgtt gttgtgaacg gcgaatacat
     5101 aaagcatgtg tcggaaacaa cggctgaccc tcgttcagcc cggcctgcag ttctgacagg
     5161 cacatgattt acgcactctt gtaagccttg tggacggttt cgaggcgggc gaaaaccgac
     5221 aaaacctgca aacaggatta atggctggca aatatattac ataaggatgt atctgtggta
     5281 agagcgaaga taagcagttg aatagatcgt tatatttgat aaaacagcat attatgctga
     5341 tttgtgtgcg gtgtctggat gattagatcg tttaaaaatt ttaggcgatc tcttctacta
     5401 aaaatgatga aaaacacagc ataaaagatc gtttgtggaa ttgtctattt ttttgacata
     5461 gttcaaaaaa acgacgtttt ttagggggtt aatgagaggc ttggtaaatc agagaaatcg
     5521 tgtcggcttt tcaaagcggt ggaaaagggg tatattgcgg atcgttattc agtggctttt
     5581 gggatcctcg cggtccggaa agtcagaaaa cggcaggatg cgccataagg cattcaggac
     5641 gtatggtaga aacgacggca gtttgccggt gccggaaggc tgaaaaaagt ttcagaagac
     5701 cataaaggaa aacccccatc atctttccac gaaacttggc ggaatcagag aaagataatg
     5761 ggggcctcac agaatacggg ataagtatat atgaaaccgt gtcagagatt caacccttcg
     5821 cagtgcatta acgcacggca ttctgttccc gccataagcg acagcttgtg gcggccctga
     5881 accagacctc attcactgaa agcacatacc gtcaggtaaa gaacccgaat ccggtgttca
     5941 caccccgtga agatgccgga acgctgaagt tctgcgaaaa actgatggaa aaggccgtgg
     6001 gcttcacctc ccgttttgat tttgccgcgc atgtggcgta tgcccgttcg cgcggcctgc
     6061 gccggcgtat gccgccggtg ctgcgccgcc gggcgattga tgcgctgctg caggggctgt
     6121 gtttccacta tgacccgctg gccaaccgcg tgcagtgctc catcaccacg ctggccattg
     6181 agtgcggact ggcgacggag tctgaggccg ggaaactctc catcacccgt gccacccgtg
     6241 ccctgaagtt cctgtctgag ctggggctga taacctacca gacggaatac gacccgacaa
     6301 ttggctgtaa catcccgacc gacatcacgt tcacgccggc actgtttgac tcacttgata
     6361 tctcagaaga ggcagtggct tccgcacgcc gcagccgcgt ggaatgggaa aacaggctgc
     6421 gcaaaaagca ggggatggat gcgctgggta tggatgaact gatagcgagg gcctggcgtt
     6481 ttgtgcgcga gcgtttccgc agttatcagg cagagcttaa gtcccacggc atgaaacgtg
     6541 cccgtgcgcg ccgcgatgca ggcaggaccc gtcaggacat tgtcaccctg gtgaaacggc
     6601 agctgacacg tgaaatcgcg gaagggcgtt tccggggaag cctggaggcc gtgaagcgcg
     6661 aaatcgaccg tcgcgtgaag gaacgcatga taatgtcgcg gaacaacaac tacacacggc
     6721 tggccacagc ctcaccctga gtttgacctc ctctgaataa tccggcctgc gccggaggct
     6781 tccgcacgtc tgaagcccga cgacgcacaa aaaagcagca cagcacacaa aaaacagcct
     6841 catcatctgc cttccggctc gccttgctcc ccctgttttt cacgcaaaac acgtctcaca
     6901 gacggggaat tctgtttatc cacatttaac tgcaagggac ttctccgtaa ggttacaacc
     6961 gttcatgcta taaagcgcca gccgcaggcg ttacagggtg caatgtttct tttaaacccc
     7021 agtacagtat ttcctttaaa ctacttaatt acttccattt aaaaagaaga cctattcagt
     7081 gcctgtcctg tggacaagca aatatgcacc tcccacgcta acgcgcgggc ccccaacctc
     7141 accgttctga aaccacagca aaaggacatc aggaataaaa acaccacaca aacgcagcac
     7201 cgtgcccatc cctcataact aaaaagcgag gccgcccccg cccgaagggc gggaacaaca
     7261 tcgcttttaa ttatgaatgt tgtaactaag gatcttcatt gctgtcagtc ttctggctag
     7321 atgttctcag tacacgctcg taagcggccc tgacggcccg ctaacgcgga gatacgcccc
     7381 gacttcgggt aaaccctcgt cgggaccact ccgaccgcgc acagaagctc tatcatggct
     7441 gaaaacgggt atggcttagc agggctgggg atgggtaagg tgaaatctat caatcagtac
     7501 cggctgacgc cgggcttcgg cggttttact cttgtgccat atgtagctac atcatgccgc
     7561 cttccatgcc gcttacgcgg catcagaaca ataaaaccag cattaaatgt atttgcaaaa
     7621 gtatgagtaa tacagacttg ttaaccggtc ctttcagatg tatctttaag acttgttaaa
     7681 cagtctcaaa ggtgcgttat gcctaacatc attctgtctg acaccagtgc aagtgtcagt
     7741 gaactgaaaa aaaatccgat ggctacagtc agtgccggag atggtttccc tgtcgctatt
     7801 cttaaccgga atcagccggc attctactgt gtgcctgcgg aactgtatga aaggatgctt
     7861 gatgcgctgg atgaccagga actggtgaaa ctggttactg aacgcagcaa tcagccactg
     7921 catgatgtgg acctggacag ttatttatga cttatacagt aaaattcagg gatgatgcgc
     7981 tcaaagaatg gctgaaactg gataaatcca ttcaacagca gtttgcgaaa aaactaaaga
     8041 aatgcagcga aaatcctcat attccttccg caaaactacg tggattaaaa gactgctaca
     8101 aaatcaaact tcacgcatcg ggatttcgtc tggtctatca ggtgattgat gacatgctaa
     8161 ttattgcggt tgtcgcggta ggtaaacgtg aacgtagtaa cgtttataat ctcgccagtg
     8221 agagaatgag atagttatct ttaaggctga ctgatattac ttcaaaagta tttctgatag
     8281 ttatgttatt ttattccaca atgcagaata tattctttat tctttaaggt cgtttttatg
     8341 atgaaaaatc cattatctta ttttcatatt gccacctttt tattgtcagg taaaagtcgt
     8401 aatctgtcag aatacgggaa agccgaagtc atcatgcggg ataagtttgt cttacaccgc
     8461 ctgataagaa acgggatgca gagacaacga ggctttctga tgtggtggcg tctggccaat
     8521 gagatgttta tctacggaaa caaaaaacaa aggaaatgtg cgataaagat aaaaaatgca
     8581 cttatggaac ggtatgggtg tgatatcggc ctgggggcgc ggattggtaa aggtctcgtg
     8641 ttgcctcatc atgcaggtat cgtcattcat ggtaatgttg atatcggtga gaatgtcatt
     8701 atacggcaga atacaaccat tggtgaaaag gtttctgaca gcagggaaaa ctatattgtt
     8761 attggcgata atgtcgatat tggtgcccat acatgtatta ttgggttaaa tgtgaaaatc
     8821 ggaagtaatg ttaaaatcgg ggcaatgtct tttattatgg aagatgtacc tgataactgt
     8881 acttatgtca ccaggaagga atccagagtg ataattcgcg aacagtaatg aaatacatgt
     8941 tatcattctt ctgttggtga ctttgccgac tcagatatat actatctgta tgtcgtgcat
     9001 tatatattaa cgatacgcag aggtgtgtat atgggcagtg ttacaaagaa accgtcacct
     9061 gttgttatta ttaagaaaaa aagacgaatt ctttctcatc aggatgatgc cgtacaggca
     9121 gttcctgatg atataaaacc agcgccagta atggaattta aaaccgactc attacctcct
     9181 gaaataccag ccacagtggc cggttccccg gaacaacaga aaaaaacagg acgtaagaag
     9241 aaaaaaaaga agaacaatag atggaatcat gagtttatac gacacagtct ggactgtgtt
     9301 aaaaaaacat tccctcagtt acgggctgaa gaggggggat atcatgcatt taaaataggc
     9361 atctcaaagg atgtcatggc ttttatttcg gccagtcctg agtgtggact aactgtaaaa
     9421 gaatggaatt gtgctgtaag gatgattact ggcggttaca aatatctgca gcgtatatca
     9481 gtcgcaggta ctccgcgtta tggtctggat ggcaaagttc atggaactgt aacggaggaa
     9541 gaagcgctgt atgcgcaggc aaaacttgaa aaacacacgc agcgttataa cgccaggatg
     9601 aaagagacag aagatgctcg ttgcaaccaa ccatcgacaa actgtcacac cggacagcaa
     9661 tttgaaggac gcctgcattc ataacggggc ataattatgt gaaatgctgt cccgctatgg
     9721 aatcagaatt gagtaacgac acgaggtttt gatgattact gttattattt ctgaggtggg
     9781 tggctggcgt gaatggaaac accgcgcccg aacaaaggat gcacagacag ccatcatccg
     9841 ggctatgaat aaacattttc cacgatctta caattttatc cctgatgata ttgataatgc
     9901 acctgtatta tttgcagctg taacacgtac accgaacgtg gaaataaccg gtcatatctg
     9961 gaaacctatg tggaacaggg gaatttgctg gaacgtcaaa ggtccgccag taatcatcac
    10021 gctcatccag ggggccgctt ggaattcgga aaataaacca cgttaagact attttttaga
    10081 caaatgactt tcaggaatcg ctaggatggc gggacaacag ctgtttcgct gtccgaaatc
    10141 gtacaattct gtacatccac gcaagagaat gtacggaagt aggccaaatg gttatcggac
    10201 ggtcagaaca tttcgtacag cctttacgtt ccgtgagtta cctggcctgg atgtaaccac
    10261 tcgttcaact caattctctt agcgtgggaa aatttccgaa ttccaagcgg ccccctactt
    10321 caggttaaaa agaacaataa aaaatgatgc taaaattgca acattttcta aataaactca
    10381 attaattaca taataaaggt gagactttta gaggaaatgg gcacgccccc cttactacgg
    10441 tgatttcata ttgaacagaa ctgttgtaca atttaattgt acaactcaaa gaggaagcac
    10501 tatgtttaca tgtaactata gccatgctcg cgataacctg gcctcgttgc tcgagctggc
    10561 ggttgatgaa aatcagcctg ttgagattca gcgccgtaaa ggtggcgcgg ctgtactagt
    10621 tgataaagac gcttttgaac gataccgtaa agcggctctt gatgctgagt ttgctgagat
    10681 catgcaaagt catgggacgg cctatacgga gctttctaag cgatgaaatt cgttagttca
    10741 caggaagtga ttgagtttca tgaccgcttg atttcgcgtg atggtggcgt ggcgggtatg
    10801 ccagaacccg gtcgcgctga cgcgatcatt catcgtgttt tgaacatgta tcattatgaa
    10861 ggtgtgacag atattatgga tctggctgcc gtgtatttag ttgctattgc tcgtggtcac
    10921 attttcaacg atgcaaataa gcgaacagcc ttgtttgttg cacaggtttt tttaaaacgg
    10981 aatggtgtac atattattag ttcacgtatt tcgtttgatg aaatgcaaat tatcgctttg
    11041 aatgctgcaa caggagaata taactggaag atggtttctg accatcttaa agccattatt
    11101 ctaaattaga tatagatatc caatcagcag cattttacaa tctactgagc aatgtaagaa
    11161 tcatatatct ttttactata taatttctgg cgtatgtgct caattcagac ctgacactct
    11221 tccgcttcct cgctcactaa cgttcggtcg ctgggcctgc ggcgagcgtt tgtaattttt
    11281 ttctaatact gacgacgtgc ttcctgatgg gtaatattgt gtaataatac acattattaa
    11341 tggttaatgt cttaaatgtg tagttaatgc taatgtatgc atacattagt atta
//
LOCUS       DAZFYC010000148        11276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFYC010000148 DAZFYC010000000
VERSION     DAZFYC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.148"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..176
                     /locus_tag="ACHKW5_004447"
     CDS             42..176
                     /locus_tag="ACHKW5_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO7513691.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            155..259
                     /gene="speFL"
                     /locus_tag="ACHKW5_004448"
     CDS             155..259
                     /gene="speFL"
                     /locus_tag="ACHKW5_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO7513692.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            570..2768
                     /gene="speF"
                     /locus_tag="ACHKW5_004449"
     CDS             570..2768
                     /gene="speF"
                     /locus_tag="ACHKW5_004449"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO7513693.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2765..4084
                     /gene="potE"
                     /locus_tag="ACHKW5_004450"
     CDS             2765..4084
                     /gene="potE"
                     /locus_tag="ACHKW5_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO7513694.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4140..5780)
                     /gene="pgm"
                     /locus_tag="ACHKW5_004451"
     CDS             complement(4140..5780)
                     /gene="pgm"
                     /locus_tag="ACHKW5_004451"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO7513695.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5806..6351)
                     /gene="seqA"
                     /locus_tag="ACHKW5_004452"
     CDS             complement(5806..6351)
                     /gene="seqA"
                     /locus_tag="ACHKW5_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO7513696.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6536..7300
                     /gene="ybfF"
                     /locus_tag="ACHKW5_004453"
     CDS             6536..7300
                     /gene="ybfF"
                     /locus_tag="ACHKW5_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7513697.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7440..7733
                     /gene="ybfE"
                     /locus_tag="ACHKW5_004454"
     CDS             7440..7733
                     /gene="ybfE"
                     /locus_tag="ACHKW5_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO7513698.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7873..8403
                     /gene="fldA"
                     /locus_tag="ACHKW5_004455"
     CDS             7873..8403
                     /gene="fldA"
                     /locus_tag="ACHKW5_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO7513699.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8613..8699
                     /gene="uof"
                     /locus_tag="ACHKW5_004456"
     CDS             8613..8699
                     /gene="uof"
                     /locus_tag="ACHKW5_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO7513700.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8692..9138
                     /gene="fur"
                     /locus_tag="ACHKW5_004457"
     CDS             8692..9138
                     /gene="fur"
                     /locus_tag="ACHKW5_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO7513701.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9222..9548)
                     /gene="chiQ"
                     /locus_tag="ACHKW5_004458"
     CDS             complement(9222..9548)
                     /gene="chiQ"
                     /locus_tag="ACHKW5_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO7513702.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9598..11004)
                     /gene="chiP"
                     /locus_tag="ACHKW5_004459"
     CDS             complement(9598..11004)
                     /gene="chiP"
                     /locus_tag="ACHKW5_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO7513703.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2741 a         2772 c         2813 g         2950 t
ORIGIN      
        1 aaataacaaa acagcatcta atatggcgct attcataatt aatgatttat tatttggggt
       61 atgatcgttt tttgttgatc ttcttcacag attatagcca tttcatggat agaataactc
      121 taccttcaac tgacacagca agaggtaaag gtaaatggaa aataataacc gcttaatgcc
      181 tcatataagg cggacaaccc atatcatgaa gtttgcccat cgtaatagtt ttgactttca
      241 tttctttaat gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga
      301 ctttaagcat tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt
      361 aaactctaaa taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt
      421 taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc
      481 ggtggagctt tgcttttttt ccggcgtgat cgatttctcc tttgagaaat tgaggacctg
      541 ctattacctg aaataaagag atgaacaaaa tgtcagaatt aaaaattgcc gttagtcgtt
      601 cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa tatagacgaa agtaattata
      661 ttgacgttgc cgccattatt ttatcagtca gtgatgttga acgtggaaaa ctcgatgaaa
      721 tagacgctac tggctatgac attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac
      781 ccgcagaata tctgccacgt atttctggtg tcttcgaaca ttgcgaatca cgtaaagagt
      841 tttatggacg tcagttagaa accgctgcca gccattatga aacgcaactg cgcccgccct
      901 tcttccgcgc actggtcgat tatgttaatc agggcaatag tgcttttgac tgccctggtc
      961 atcagggggg tgagtttttc cgtcgccatc cggcaggcaa tcagttcgtg gaatactttg
     1021 gcgaaatgct cttccgctct gacttgtgta atgctgacgt agcaatgggc gatctgctga
     1081 ttcatgaagg tgcgccatgt attgcacaac agcatgcggc taaagtcttt aatgccgata
     1141 aaacctattt cgttttaaac ggtacttcat cttctaataa agtggtctta aacgcattgc
     1201 tgacgccggg cgatctggtg ttgtttgacc gcaataacca taaatctaac caccacggtg
     1261 cgttgctaca ggctggcgcg acaccggttt atctggaaac cgcacgtaac ccgtatggct
     1321 ttatcggtgg cattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg
     1381 aagtcgcacc gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag
     1441 gcacctacga cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt
     1501 gtgactacat tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg
     1561 ctgactgttc gccgctgttg ctggatctta atgagaacga tccgggtatt ctggttacgc
     1621 aatccgtaca taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca
     1681 gccacatcaa agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga
     1741 tgcatgcctc caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa
     1801 tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg
     1861 cccgcaagct gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg
     1921 atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc
     1981 agtttgtacc aggtgagcac tggcactctt ttgaaggcta tgcagagaat caatactttg
     2041 tcgatccatg caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg
     2101 aagcgttcgg tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc
     2161 cggaaaaatg cgatcttaac tccatcctct tcctgctgac tccggcagaa gatatggcca
     2221 agcttcagca acttgttgcc ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc
     2281 cattagcaga agtgctgcct tccatctaca aacagcatga agagcgctac gccggttata
     2341 ccctgcgtca gttgtgtcaa gaaatgcatg atttgtatgc ccgtcacaac gtgaaacaac
     2401 tgcaaaaaga gatgttccgt aaggaacact tcccacgcgt aagcatgaat ccgcaagaag
     2461 ccaactacgc ctatttacgc ggtgaagtgg agctggttcg tctgccggat gcagaaggcc
     2521 gtatcgctgc cgaaggtgcg cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg
     2581 aaatctgggg tggtgctgtt ttgcgttact tcagcgctct ggaagaaggg atcaacctgc
     2641 tgccaggttt tgcaccggag ctgcagggtg tctatatcga agaacatgat ggtcgtaagc
     2701 aagtttggtg ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa
     2761 aattatgagt caggctaaat cgaacaaaat gggcgtcgtt cagttaacca tactgacgat
     2821 ggtcaacatg atgggctccg gtatcatcat gctgccgaca aagcttgccg aagttgggac
     2881 aatttcaatt atctcctggc tggtgacagc cgtaggctca atggcactgg catgggcatt
     2941 cgcaaaatgc ggtatgttca gccgtaaatc aggcggtatg ggcggttatg cggagtatgc
     3001 tttcggtaaa tccggtaact ttatggcgaa ctatacctac ggcgtctcgc tgctgattgc
     3061 taacgtcgcg attgctattt cggcggttgg ttacggcacc gaactgctcg gcgcgagttt
     3121 gtcgccagtg cagattggtc ttgcgaccat cggggtgctg tggatttgta ccgtggctaa
     3181 ctttggtggt gcgcgcatta ccgggcaaat cagtagcatt accgtgtggg gggtcattat
     3241 tccggtggta ggcctgtgca ttatcggctg gttctggttt agcccgacgc tttacgttga
     3301 ttcctggaat ccgcatcatg caccgttctt cagtgcagta ggttcttcca tcgctatgac
     3361 gctgtgggct tttctcggtc tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc
     3421 ggaacgtaat gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg tgatttatat
     3481 cgtctccacc aacgtgattg cagggattgt gcctaatatg gagctggcaa attcaacggc
     3541 accatttggc ctggccttcg cacagatgtt cacaccagaa gtgggtaaag tcattatggc
     3601 gctgatggtg atgtcctgct gcggttcgct gcttggctgg cagttcacca ttgcccaggt
     3661 gtttaaatct tcatctgatg aaggctactt ccctaaaatt ttctcccgtg taaccaaagt
     3721 ggatgcaccg gtgcagggaa tgttgaccat tgtgattatc cagagcgggt tggcgctgat
     3781 gacgattagc ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg ccgtggtgac
     3841 caatatcatt ccgtatattc tgtcaatggc ggcattagtc attattcaga aggtcgctaa
     3901 tgtgcccccc tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg cgatgtatag
     3961 cttttatgcg ctgtactcat ccggggaaga agccatgctg tacggttcca tcgtgacctt
     4021 ccttggttgg acactgtatg gtctggtatc accacgcttt gaactgaaaa ataaacacgg
     4081 ttaaaaaagc aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat taaatgtgtt
     4141 tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga
     4201 tgttcttcac cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga
     4261 cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccgataga agcaccatta
     4321 cccggtgccg ccgtcagacg tgcggtgatc gggtcacctg ccagggtgct ggcgctcacc
     4381 atttccggag acagcttaga caacgccgct ttttgtgcgg aagtcgcagc ggcctgcaaa
     4441 cggttgtagc tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc
     4501 ttaccggtga cagcggtgat ttccgccgcc agcagacaca taatgatgcc gtctttatcg
     4561 gtggaccacg gcgtgccgtc gaaacgcagg aaggaagccc cggcgctctc ttcgccgcca
     4621 aagccgaaac tgccgtcgaa cagaccatca acaaaccatt tgaatcctac cgggacttct
     4681 accagtttac ggcccaaggc gttgaccaca cggtcgatca tcgcagaaga aaccagcgtt
     4741 ttaccgacag caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc
     4801 accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca
     4861 taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag caggcccgcc
     4921 atcgcacact cggaggagca gtccatacgg atcgcgccgt ctttatcaag gtgcataaag
     4981 cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca
     5041 atacgcttcc agtattcgat accggaaccg cccagcggat caacgccaag cgtcaggccc
     5101 gctttctgaa tcgccgccat atcaacgata tcggccagac cttccacaaa cggctgcacc
     5161 agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagagagat acgcttcacg
     5221 cctttcaggc catcggccat cagtgcgttg gccctgtctt ccaccacttt ggtgacgttg
     5281 gtatcagccg ggccaccatt tggcgggttg tacttgatac caccatcttc cggcgggtta
     5341 tgggacggtg taatcacgat accgtctgcc agcgggccac cttttttatt gtgaaccagg
     5401 atggcattgg aaacggcagg cgtcggagtg aatccattgt tttcctgcac aatgacatca
     5461 acgccgttcg ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga
     5521 gtatctttac ccacatagca agggccagtg atgccatttt tcgcacgttc ttcagcaatt
     5581 gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg
     5641 tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca
     5701 taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct
     5761 gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgcg
     5821 aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct
     5881 gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt
     5941 cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg
     6001 tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag
     6061 tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcgtccga
     6121 aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc
     6181 gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatga
     6241 ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt
     6301 gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca
     6361 gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc cggcagcgga
     6421 tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg
     6481 gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa
     6541 attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt
     6601 ccatggtctg tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga
     6661 tcacaatatc atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat
     6721 gaattacccg gcgatggcgc aggatcttgt tgatactctg gatgcacagc agatcgacaa
     6781 agcaacattt atcggtcact ccatgggcgg taaagcggta atggcactta ctgcactggc
     6841 ccccgatcgc atcgataaac tggtggcgat cgatatcgca ccggtcgact atcacgtacg
     6901 ccgtcatgat gagattttcg cggctatcaa cgcggtcagt gaatcggacg cacaaactcg
     6961 ccagcaagca gcagcaataa tgcgccagca tcttaatgaa gaaggggtga ttcagtttct
     7021 gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta
     7081 tccgcatatt gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc
     7141 tggcggcaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc
     7201 acaggcacgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga
     7261 tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca
     7321 gcgtccgcgt cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg
     7381 gcgcgctatc catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca
     7441 tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg
     7501 gacgaccgaa aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc
     7561 agctaaaacg cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag
     7621 ctgtcgaggc gctgaacgag ctggcggagt cgcgcaatat gagccgtagc gaactgatcg
     7681 aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca
     7741 ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc
     7801 tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag
     7861 gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa
     7921 aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt
     7981 gcaaaaagca gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc
     8041 tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt
     8101 gatttcaacg gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa
     8161 tatttctgcg acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaaccatc
     8221 gttggtcact ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggcagatgac
     8281 gaccactttg tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt
     8341 gtagaaaaat gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tcttaatgcc
     8401 tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat
     8461 aattgctaca aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg
     8521 gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact
     8581 tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt
     8641 ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat
     8701 aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttccacgttt aaaaatcctg
     8761 gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg
     8821 atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac
     8881 gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca
     8941 cagcaacatc accacgatca cctgatttgc ctcgactgcg gcaaggttat cgaatttagt
     9001 gatgattcca tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact
     9061 aaccacagtc tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgagcac
     9121 gcgcacgaag gcaaataagc cagcctgaac gagaaaagcc aacctgcggg ttggcttttt
     9181 tatacaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt attactgcgt
     9241 atttcctgcc agaccttatc acaatcggct ttcaccgcct gatcatttcc ggtttgcgtt
     9301 gcacgcagac actgctgata atccagtacg cgtacacttt cctggtcggc aaactgctga
     9361 tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat tttttctggc
     9421 gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacgact gtcttcctgt
     9481 ggcgcggttg attgcgcaca agctaccagc cccgatgcca ttatggcgat gagaatcagt
     9541 tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg tcgggcatca
     9601 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
     9661 accgtaaccg ccgccccagc ttgggatgtc agagtggttg tcgtattcgg tgaagtgcag
     9721 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
     9781 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
     9841 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
     9901 ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt tggcgttgaa
     9961 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggagt
    10021 catacgttgc aggaagtatc cctgctgacc gtcagcctta acccaggtgc cttcgaggcg
    10081 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    10141 gtcataaagg tcgttgacgc tgcgatcgtc aactttatcg cgcgtaccat agaactggta
    10201 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    10261 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    10321 gtatttcgca ccgatggagt gcaggtaatc aactttggtg gttttatcgt tctgataaaa
    10381 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    10441 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    10501 tggcataaag ctccagtgcg gcgctaacag cgtttgacca gttggctgaa tgtaacctgc
    10561 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    10621 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    10681 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    10741 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    10801 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    10861 ccagtaatat ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    10921 cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg ccagcgtact
    10981 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    11041 aaggtgaaca gcgaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt ctcgctgact
    11101 acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta
    11161 agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa gtttgcggtt
    11221 gaaaggcttg cagaagaata aaagcgtgaa tttccagcaa gatgaaagcg gttaca
//
LOCUS       DAZFYC010000149        11008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFYC010000149 DAZFYC010000000
VERSION     DAZFYC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.149"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..1387
                     /gene="manD"
                     /locus_tag="ACHKW5_004460"
     CDS             173..1387
                     /gene="manD"
                     /locus_tag="ACHKW5_004460"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO7513704.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1454..2737
                     /locus_tag="ACHKW5_004461"
     CDS             1454..2737
                     /locus_tag="ACHKW5_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7513705.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2988..3905
                     /gene="oxyR"
                     /locus_tag="ACHKW5_004462"
     CDS             2988..3905
                     /gene="oxyR"
                     /locus_tag="ACHKW5_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO7513706.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3888..5288)
                     /gene="sthA"
                     /locus_tag="ACHKW5_004463"
     CDS             complement(3888..5288)
                     /gene="sthA"
                     /locus_tag="ACHKW5_004463"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO7513707.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5619..6269
                     /gene="fabR"
                     /locus_tag="ACHKW5_004464"
     CDS             5619..6269
                     /gene="fabR"
                     /locus_tag="ACHKW5_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO7513708.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6269..6628
                     /gene="yijD"
                     /locus_tag="ACHKW5_004465"
     CDS             6269..6628
                     /gene="yijD"
                     /locus_tag="ACHKW5_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO7513709.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6668..7768)
                     /gene="trmA"
                     /locus_tag="ACHKW5_004466"
     CDS             complement(6668..7768)
                     /gene="trmA"
                     /locus_tag="ACHKW5_004466"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO7513710.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7882..8072
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8137..9981
                     /gene="btuB"
                     /locus_tag="ACHKW5_004467"
     CDS             8137..9981
                     /gene="btuB"
                     /locus_tag="ACHKW5_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO7513711.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSSGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9926..10783
                     /gene="murI"
                     /locus_tag="ACHKW5_004468"
     CDS             9926..10783
                     /gene="murI"
                     /locus_tag="ACHKW5_004468"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO7513712.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2540 a         2609 c         2944 g         2915 t
ORIGIN      
        1 tttaacgata atggttaatt attcaggtgg tttttgaggg tattttttct cctcggtgga
       61 aatttttgtt tttaatgtga tcacggttga ataatctctt tttctctctt gtatggtagt
      121 aggcgtaaaa ttaagtttga aacgtctatt tacaacacag gagattgcaa taatgaaaat
      181 tattgctgca gatgtttttg ttacttgccc agggcgtaat ttcgttacgc ttaaaataac
      241 cactgaaagc ggactttgtg gattaggaga tgccacgtta aatggtcgcg aattgtcggt
      301 cgcttcttat ctgaaagatc atctttgtcc acaactaatt ggccgcgatg ccagccgtat
      361 cgaagatatc tggcaattct tttataaagg tgcttactgg cgacgtggcc ccgtcacgat
      421 gtcagccatt tctgccattg atatggcgtt gtgggatatc aaagctaaag cggcaaatat
      481 gccattgtat caactgcttg gtggtgcttc tcgcgaaggc gtgatggttt attgccacac
      541 caccgggcgg acgattgatg aagtccttga agactatgca aagcaccagc agatgggctt
      601 taaggcgatt cgtgtgcagt gcggtgtacc gggtatgcaa actacttatg gcctggcgaa
      661 gggcaaaggc ctggcgtatg aaccggcaac caaaggcctg tggccagaag agcagttgtg
      721 gtcttcagaa aagtatctcg attttacgcc gaagttattt gaagcggtgc gtaataagtt
      781 cggttttaac gaacatctgt tgcatgacat gcaccatcgc ctgaccccca tcgaagcggc
      841 gcgctttggg aagagcattg aggattaccg cctgttctgg atggaagatc cgacgcctgc
      901 agagaaccag gaatgttttc gtctgattcg ccagcatacc gtgacgccga ttgcggtcgg
      961 tgaagtgttt aacagtatct gggactgcaa gcagttaatc gaagagcagc tgattgacta
     1021 tattcgcacg acgatcaccc atgcaggcgg cattaccggg atgcggcgga ttgccgattt
     1081 tgcatcgctt tatcaggtac gtaccggttc gcatggccct tcagatttat cgcctgtttg
     1141 tcatgcggcg gcgctgcatt ttgacctatg ggtaccaaat tttggcgttc aggagtacat
     1201 gggctattcc gaacaaatgc ttgaagtttt cccgcacagc tggcgcttcg aggaaggtta
     1261 tatgcaccca ggcgatgaac cggggctggg tatttctttc gatgagaaac tggcggcgaa
     1321 atatccgtat gacccggcgt atctccccgt cgcacgactg gaagatggca cattgtggaa
     1381 ctggtaataa cggttcgctt aattattttc tgaatagtca tcaaatcgat taattccgct
     1441 caaggagcta aaaatggacg ttgatgtgtc aaccagtgtt gcgggtaata aaccgcaacg
     1501 gattcgtcgt attcagacag tgaccctggt tttattattt atggcgggga tcgttaattt
     1561 tctcgaccgt tcgtcattga gcgtggcagg tgaagcgatt cgtggcgagt taggattatc
     1621 ggcgacagag tttggtgttt tgctttctgc attttctctg tcttatggtt tttcacaact
     1681 accttccggt attttgctag accgctttgg cccacgaatt gttttaggcg caggcttaat
     1741 attctggtca ttaatgcagg cattaacagg aatggttaat tcttttagcc actttatttt
     1801 aatgcgtatc ggtctgggta ttggcgaagc gccatttatg cctgcgggag taaagtcgat
     1861 caccgactgg tatgcacaaa aagaacgcgg cacggcgctg gggattttta actcatctac
     1921 cgttatcggt caggctatcg cgcctcctgc tcttgtattg atgcagctgg catggggctg
     1981 gcggacgatg ttcgttatca tcggcgtggc agggattctg gttgggatct gttggtacgc
     2041 gtggtatcgc aaccgggcgc agtttgtcct gactgacgaa gaacggacgt atctctccgc
     2101 gccggttaaa ccgcgtccac agctgcaatt tagcgagtgg ctggcgctgt ttaagcatcg
     2161 gacaacctgg gggatgattt taggtttctc tggcgtcaat tataccggtt ggctctacat
     2221 cgcgtggtta cccggttatt tgcaagccga gcaaggtttc agtctggcga aaaccggttg
     2281 ggtggcggcg attcctttcc tcgcggcggc agtcgggatg tgggttaacg gtattgttgt
     2341 cgatcgactg gcgaaaaaag gctacgacct ggcgaagacg cgtaaaacgg ctattgtctg
     2401 cggtttgatg atgtcggcat taggcacgtt gctggtcgtg caatcttcct cgccagccca
     2461 ggcggtagcg tttatctcaa tggcgctatt ctgcgtgcat ttcgctggaa cgtctgcatg
     2521 ggggctggtg caggtgatgg tgtcagaaac aaaagtggct tccatcgccg ggattcaaaa
     2581 ctttggcagt tttgtttttg cttcctttgc tccgatcgta accggttggg tagtggatac
     2641 cacacactcg tttaatctgg cgttggttat cgcggcctgt gtgacgttca ccggggcgct
     2701 gtgttacttc tttattgtca aagatcgcat tgagtaatcg ttactatcag gctctcttgc
     2761 tgtgggcctg tagaataaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt
     2821 tagttattcg agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg
     2881 ccgctccgtt tctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg
     2941 ctattctacc tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg
     3001 atcttgagta cctggtggca ttggccgagc accgccattt tcggcgtgcg gcagattcct
     3061 gccacgttag ccagccgacg cttagcgggc aaattcgtaa gctggaagat gagttgggcg
     3121 tgatgttgct ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg
     3181 tagatcaggc gcgtaccgta ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc
     3241 agggcgagac gatgtccggg ccgctgcaca ttggtttgat tcccacagtt ggaccgtacc
     3301 tgctaccgca tattatccct atgctgcacc agacctttcc aaagctggaa atgtatctgc
     3361 atgaagcaca gacccaccag ttactggcgc aactggacag cggcaaactc gattgcgtga
     3421 tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa
     3481 tgttgctggc tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ccgatggccg
     3541 atctggcagg ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa
     3601 tgggtttctg ttttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg
     3661 agacgctgcg caacatggtg gcggcaggta gcgggatcac tttactgcca gcgctggctg
     3721 tgccgccgga gcgcaaacgc gatggggttg tttatctgcc gtgcattaag ccggaaccac
     3781 gccgcactat tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc
     3841 tggcagaggc catccgagca agaatggatg gccatttcga taaagtgtta aaacaggcgg
     3901 tttaaaccgt ttaacgcagc tacccgatag gcttccgcca tggtcgggta gttaaaggtg
     3961 gtgttgacga agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg
     4021 atatgaataa tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt
     4081 tcccgatgga acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcgcgtgcc
     4141 agatgtttaa actgggcgcg gcccacttca tatggcactt tcatcgcggt cagctgctgt
     4201 tcggttttgc ccacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca
     4261 atcagatgcg cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca
     4321 taggccgctg atgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc
     4381 tgtgcggtct gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca
     4441 atgttctgta acgccagcga atcggtatta ccggtacgac cgttggcata gagcaggcag
     4501 tcagctttca gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagccttcg
     4561 atcttctcgt actcttcgtt gtgacgaatc actacgccac tgttccagaa gtgataggag
     4621 agagaatctg acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga
     4681 tccactttta catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca
     4741 ccatagataa gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa
     4801 atgcgtggat gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata
     4861 acaaattttt cagcggttag tgtttcaacg ctgccgtccg ggcaatccag cgccaacgta
     4921 tgctcgtcaa caaagcgagc gtttccctgc aatatttcac agtgattacg ttcgtaaaat
     4981 ccctgacgca tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca
     5041 aaagaagagc ggagcagtcg ggaatggtcg ctgtaaagtg ggttttgatt gaattctata
     5101 atgcggctga cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg
     5161 ccgccaacat tttgataacg ctcgataact gcgacgcgcg caccttgctt aaccaggccc
     5221 attgcagcgc cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa
     5281 tgtggcatgg tagggcttac ctgttcttat acataaaagc aacagaatgg taacatttta
     5341 tcgcgggtaa gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct
     5401 ttgtctgttt cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg
     5461 tttaagcggc aggtttccgc tgtacgtaaa agaaccggcc aaagaattgc agtaaatatg
     5521 ttttattgcg ttaccgttca ttcacaatac tggagcaatc cagtatgttc attctctggt
     5581 atagtgccag cagtactttt ggcaaggatt cagacatcgt gatgggcgta agagcgcaac
     5641 aaaaagaaaa aacccgccgt tcgctggtgg aagccgcatt tagccaatta agtgctgaac
     5701 gcagcttcgc cagcctgagt ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct
     5761 ctttttatcg gcatttccgc gacgtagacg aactgggtct gaccatggtt gatgagagcg
     5821 gtttaatgct acgccaactc atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg
     5881 tgatccgcac ctcggtctcc acatttatgg agttcatcgg taataatcct aacgccttcc
     5941 ggttattatt gcgggaacgc tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg
     6001 aaattcagca cttcattgcg gaacttgcgg actatctgga actcgaaaac catatgccgc
     6061 gtgcgtttac tgaagcgcaa gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg
     6121 aggcgttgga cgtcggcgtc gaacaacgtc ggcaattaga agagcgactg gtactgcaac
     6181 tgcgaatgat ttcgaaaggg gcttattact ggtatcgccg tgaacaagag aaaaccgcaa
     6241 ttattccggg aaatgtgaag gacgagtaat gaaacaagca aatcaagata gaggtacgct
     6301 gctgctggcg ttggttgctg gcttatcgat taatggtact ttcgcagcgc tgtttagctc
     6361 cattgtgcca ttttctgtat tcccgattat ttccctggtg ctgacggttt actgcctgca
     6421 tcaacgttat cttaatcgca ccatgccggt aggcttgccg ggtctggcag ctgcctgttt
     6481 tattctcggc gtactgctgt acagcacggt agttcgtgcg gaatatccgg atatcggctc
     6541 taacttcttc ccggcagtac tctccgtcat tatggtgttc tggattggcg cgaagatgcg
     6601 taaccgtaag caggaagttg ctgagtaatc ggtattatgc cggatgctgg cgcatccggc
     6661 atgggtttta cttcgcggtc agtaatacgc cgcactccat atggtgcgtg taggggaact
     6721 gatcaaacag agccagacgt tcgaccttgt gcgtctggct taatgtttcc agattcttgc
     6781 ataacgtttc cggattgcag gagatataca gaatacgtgg atacgcctgc accattttct
     6841 cggtttcact gtccagaccg ctgcgcggtg ggtcgacaaa aatggtttcg cactgataac
     6901 tcttcaagtc tatcccttgc aggcgattaa actcgcgtac accattcatc gcctgagtaa
     6961 attcttctgc cgccatacga ataatttgta cgttatcaat atggttagct gcgatgttgt
     7021 attgcgcagc agcaaccgac ggcttagcga tttcggtggc taatacccga tcaaaattgc
     7081 gcgccagtgc taatgaaaag ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg
     7141 agcctttggt tacgtccagc gcccattcca gcatctgaat attcatcgcc gcgttcggct
     7201 gggtaaaact gttttctacc tgacggtaga tcatctcttt ccctgcgacc ggcagacgtt
     7261 catcgatgta atcttgatcc agctcgattt tggttttcgt tgcccgacca atcagatgca
     7321 cattcagatt ctgcgcgcgc agtgcatcgc gcagggcttc cgcttcctga cgccactcat
     7381 catccagctt tttatggtac aacagggaaa ccaccgcctg attactcagc gtggtgaggt
     7441 aatcaatctg gaacaacttg tggcgcagaa cgggattatt acgcacaccc gcaatcatcg
     7501 ccgtcatcaa ctggttgatg agttcactgg cagcggggaa gctatccacg cggatgcggc
     7561 ttttggtttg ttgatcgaaa atgatgtgat acaggtcatc gccatcgtgc cagatgcgga
     7621 actccgcacg catccggtaa tgactgaccg gcgagcgaaa cacttccgga accaggtcag
     7681 aaaacggtgc catcatactt tgcaaacgta ccactttttc ggctaactgc gcttcatact
     7741 gttctgttgg aaggtgttcg ggggtcatga tgtatcctga aaaattaaag tacgcggcga
     7801 ttgtagggat tgctcatcag atgtccagat cttgatgaat tcctatttgt gagctacgtc
     7861 tggacagtaa cttgttacaa cctgtagcat ccacttgccg gtcctgtgag ttaataggga
     7921 atccagtgcg aatctggagc tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat
     7981 gcggacactg ccatccggtg ggaagtcatc atctcttagt atcttagata cccctccaag
     8041 cccgaagacc tgccggccaa cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa
     8101 cctgcggcat ccttcttcta ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg
     8161 acggcgtgtt ctgtcacagc cttttccgct tgggcacagg ataccagccc ggatactctc
     8221 gtcgttactg ctaaccgttt tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt
     8281 gtgacccgtc aggatatcga ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt
     8341 cttccgggcg tcgatatcac ccaaaacggc ggttcaggtc agctctcatc tatttttatt
     8401 cgcggtacaa atgccagtca tgtgttggtg ttaattgatg gcgtacgcct gaatctggcg
     8461 ggggtgagtg gttctgccga ccttagccag ttccctattg cgcttgtcca gcgtgttgaa
     8521 tatatccgtg ggccgcgctc cgctgtttat ggttccgatg caataggcgg ggtggtgaat
     8581 atcatcacga cgcgcgatga acccggaacg gaaatttcag cagggtgggg aagcaatagt
     8641 tatcagaact atgatgtctc tacgcagcaa caactggggg ataagacacg ggtaacgctg
     8701 ttgggcgatt atgcccatac tcatggttat gatgttgttg cctatggtaa taccggaacg
     8761 caagcgcaga cagataacga tggtttttta agtaaaacgc tttatggcgc gctggagcat
     8821 aactttactg atgcctggag cggctttgtg cgcggctatg gctatgataa ccgtaccaat
     8881 tatgacgcgt attattctcc cggttcaccg ttgctcgata cccgtaaact ctatagccaa
     8941 agttgggacg ccgggctgcg ctataacggc gaactgatta aatcacaact cattaccagc
     9001 tatagccata gcaaagatta caactacgat ccccattttg gtcgttatga ttcgtcggcg
     9061 acgctcgatg agatgaagca atacaccgtc cagtgggcaa acaatgtcat cgttggtcac
     9121 ggtagtagtg gtgcgggtgt cgactggcag aaacagacta cgacgccggg tacaggttat
     9181 gttgagaatg gatatgatca acgtaatacc ggcatctatc tgaccgggct gcaacaagtc
     9241 ggcgatttta cctttgaagg cgcagcacgc agtgacgata actcacagtt tggtcgtcat
     9301 ggaacctggc aaaccagcgc cggttgggaa ttcatcgaag gttatcgctt cattgcttcc
     9361 tacgggacat cttataaggc accaaatctg gggcaactgt atggcttcta cggaaatccg
     9421 aatctggacc cggagaaaag caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg
     9481 gtgaactggc gtatttccgg atatcgtaac gatgtcagtg acttgatcga ttatgatgat
     9541 cacaccctga aatattacaa cgaagggaaa gcgcggatta agggcgtcga ggcgaccgcc
     9601 aattttgata ccggaccact gacgcatact gtgagttatg attatgtcga tgcgcgcaat
     9661 gcgattaccg acacgccgtt gttacgccgt gctaaacagc aggtgaaata ccagctcgac
     9721 tggcagttgt atgacttcga ctggggtatt acttatcagt atttaggcac tcgctatgat
     9781 aaggattact catcttatcc ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat
     9841 cttgcggttg cgtatccggt cacctctcac ctgacagttc gtggtaaaat agccaacctg
     9901 ttcgacaaag attatgagac agtctatggc taccaaactg caggacggga atacaccttg
     9961 tctggcagct acaccttctg aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg
    10021 tgggttgtcg gtctatgacg agatccggca tctcttaccg gatctccatt acatttatgc
    10081 tttcgataac gtcgcttttc cgtatggcga aaaaagcgaa gagtttattg ttgagcgagt
    10141 ggtggcaatt gtcaccgcag tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg
    10201 caacaccgcc agtaccgttt cacttcctgc attacgcgaa aagttcgact tcccggttgt
    10261 tggtgtcgtg ccggcgatta aacctgctgc acgtctgacg gcaaatggca ttgtcggatt
    10321 actggcaacc cgcggaactg ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc
    10381 taatgaatgc cagatagaaa tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa
    10441 gctacatggc gaagatgttt ctctggatgc actaaaacgt atcctacgcc cgtggttaag
    10501 aatgaaagag ccgccagata ccgttgtatt gggttgcacc catttccctc tactacaaga
    10561 agaactctta caggtgctgc cagagggaac ccggctggtg gattctggcg cagcgattgc
    10621 tcgcagaacg gcctggctgt tagaacatga agctccggat gcaaaatctg ccgatgcgaa
    10681 tattgccttt tgtatggcaa tgacgccaga agctgaacaa ttattgcccg ttttacagcg
    10741 ttacggcttc gaaacgctcg aaaaactggc agttttaggc tgatttggtt gaatgttgcg
    10801 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    10861 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaac
    10921 tccgacagag aaagcaaaaa taaatgcttg actctgtagc gggaaagcgt attatgcaca
    10981 ccccgcgccg ctgagaaaaa gcgaagcg
//
LOCUS       DAZFYC010000150        10996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFYC010000150 DAZFYC010000000
VERSION     DAZFYC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.150"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..688)
                     /locus_tag="ACHKW5_004469"
     CDS             complement(<1..688)
                     /locus_tag="ACHKW5_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFO7513713.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQ"
     gene            complement(694..984)
                     /locus_tag="ACHKW5_004470"
     CDS             complement(694..984)
                     /locus_tag="ACHKW5_004470"
                     /inference="COORDINATES: protein motif:HMM:NF017990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="HFO7513714.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASA
                     SSYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
     gene            complement(1096..1593)
                     /gene="dfrA12"
                     /locus_tag="ACHKW5_004471"
     CDS             complement(1096..1593)
                     /gene="dfrA12"
                     /locus_tag="ACHKW5_004471"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /protein_id="HFO7513715.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGK
                     VVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVA
                     GGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVY
                     ARRNG"
     gene            1738..2751
                     /gene="intI1"
                     /locus_tag="ACHKW5_004472"
     CDS             1738..2751
                     /gene="intI1"
                     /locus_tag="ACHKW5_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO7513716.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2954..3304
                     /locus_tag="ACHKW5_004473"
                     /pseudo
     CDS             2954..3304
                     /locus_tag="ACHKW5_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            3430..3705
                     /locus_tag="ACHKW5_004474"
                     /pseudo
     CDS             3430..3705
                     /locus_tag="ACHKW5_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3708..3899
                     /locus_tag="ACHKW5_004475"
                     /pseudo
     CDS             3708..3899
                     /locus_tag="ACHKW5_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3957..4199
                     /locus_tag="ACHKW5_004476"
     CDS             3957..4199
                     /locus_tag="ACHKW5_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO7513717.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(4231..4881)
                     /gene="tetR(A)"
                     /locus_tag="ACHKW5_004477"
     CDS             complement(4231..4881)
                     /gene="tetR(A)"
                     /locus_tag="ACHKW5_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO7513718.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4987..6186
                     /gene="tet(A)"
                     /locus_tag="ACHKW5_004478"
     CDS             4987..6186
                     /gene="tet(A)"
                     /locus_tag="ACHKW5_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO7513719.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(6218..7102)
                     /locus_tag="ACHKW5_004479"
     CDS             complement(6218..7102)
                     /locus_tag="ACHKW5_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO7513720.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(7240..7632)
                     /locus_tag="ACHKW5_004480"
     CDS             complement(7240..7632)
                     /locus_tag="ACHKW5_004480"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFO7513721.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            7636..9387
                     /locus_tag="ACHKW5_004481"
                     /pseudo
     CDS             7636..9387
                     /locus_tag="ACHKW5_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            complement(9422..10252)
                     /locus_tag="ACHKW5_004482"
                     /pseudo
     CDS             complement(9422..10252)
                     /locus_tag="ACHKW5_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10913..>10996
                     /locus_tag="ACHKW5_004483"
     CDS             10913..>10996
                     /locus_tag="ACHKW5_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO7513722.1"
                     /translation="MCGRFSQSMTREEYLSLLANEADRNIAY"
BASE COUNT         2256 a         3326 c         3269 g         2145 t
ORIGIN      
        1 cttgcttagc ttcaagtaag acgggctgat actgggcagg taggcgtttt attgcccagt
       61 cggcagcgac atccttcggc gcgattttgc cggttattgc gctgtaccaa atgcgggaca
      121 acgtaagcac tacatttcgc tcatcgccgg cccagtcggg ctgcgagttc catagcttca
      181 aggtttccct cagcgcctcg aatagatcct gttcaggaac cgggtcaaag aattcctccg
      241 ctgccggacc taccaaggca acgctatgtt ctcttgcttt tgtaagcagg atagctagat
      301 caatgtcgat catggctggc tcgaagatac ccgcaagaat gtcattgcgc tgccattctc
      361 caaattgcag ctcgcgctta gccggataac gccacgggat gatgtcgtca tgcacgacaa
      421 gggtgacttc tatagcgcgg agcgtctcgc tctcgccagg gaaagccgaa gcctccataa
      481 gatcattgag caatgctcgc cgcgtcgttt catcaagctt tacggccaca gtaaccaaca
      541 aatcaatatc gctgtatggc ttcaggccgc catccactgc ggagccgtac aaatgcacgg
      601 ccagcaacgt tgattccaga tggcgctcaa tgacgcttag cacctctgat agttggttcg
      661 aaatttcgat ggtcaccgct tccctcatga tgtctaacgg gcgaggtaag ccgaccgcag
      721 aatgcgggtc ggcttgaccg aaatgttaga accagaagcc aaaacggata acttgaattt
      781 ggcgacgggc gctaaccgtg aaaaaacgct gcgccaccga ggcggcacag cactgcaaaa
      841 acgatagctg cttgcgcttg ctacgcaagg gctagaggcc aaaaagactg aaaacctgcg
      901 cagcccatgc aggcgaagcc cggaaaaaag gcaaaacagg cactgaataa cgcctgaaaa
      961 gctaaatgcc gtttgaataa acatgagcta aataaagctg ggtttcagtg gtgctaacgt
     1021 tggacgtaac gagagccgga gcgcagcgga gggaaccaaa atgcgcagca ttttggcgtc
     1081 ccgttgacgg aatggttagc cgtttcgacg cgcataaacg gagtgggtgt acggaattac
     1141 agcttgaatg gtttcggttg agacaagctc gaattctgtt tcgttgagca ttgggaagaa
     1201 ggcgtcaccc tcgaaggttt gatgtacctc agatagaaac acgccgtggg cgtgaggtag
     1261 tgccagagtg tatatctcag ctccgcccgc gacgtagagt tcattgccga gttcggatgc
     1321 caaagcgata gcgtgcgaca gcgttgaaac aactacgcag ccagtggcgc ggtagttagc
     1381 ttggcgtgag attaccaatg tgtgacggtt cggtagaggc ttgccgatag actcaaaggt
     1441 ctttcgcccc atgacaacga cttttccctc agtgagtctg cgaaaaatct tctgctcacc
     1501 cggaattttc caggggatat taggaccatt gccaataacc cgattggctc ccatcgcagc
     1561 aacgagataa atgcgtactg attccgagtt catatggcta actttgtttt agggcgactg
     1621 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     1681 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     1741 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     1801 gagcgcatac gctacttgca ttacagctta ccaaccgaac aggcttatgt ccactgggtt
     1861 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     1921 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     1981 gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt
     2041 caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa
     2101 gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca gcttctgtat
     2161 ggaacgggca tgcggatcag tgagggtttg caactgcggg tcaaggatct ggatttcgat
     2221 cacggcacga tcatcgtgcg ggagggcaag ggctccaagg atcgggcctt gatgttaccc
     2281 gagagcttgg cacccagcct gcgcgagcag ctgtcgcgtg cacgggcatg gtggctgaag
     2341 gaccaggccg agggccgcag cggcgttgcg cttcccgacg cccttgagcg gaagtatccg
     2401 cgcgccgggc attcctggcc gtggttctgg gtttttgcgc agcacacgca ttcgaccgat
     2461 ccacggagcg gtgtcgtgcg tcgccatcac atgtatgacc agacctttca gcgcgccttc
     2521 aaacgtgccg tagaacaagc aggcatcacg aagcccgcca caccgcacac cctccgccac
     2581 tcgttcgcga cggccttgct ccgcagcggt tacgacattc gaaccgtgca ggatctgctc
     2641 ggccattccg acgtctctac gacgatgatt tacacgcatg tgctgaaagt tggcggtgcc
     2701 ggagtgcgct caccgcttga tgcgctgccg cccctcacta gtgagaggta gggcagcgca
     2761 agtcaatcct ggcggattca ctacccctgc gcgaaggcca tcggtgccgc atcgaacggc
     2821 cggttgcgga aagtcctccc tgcgtccgct gatggccggc agcagcccgt cgttgcctga
     2881 tggatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     2941 tctgaaaacg acaatggagg tggtagccga gggtgtggaa acacccgact gccttgcgtg
     3001 gttgcggcag gcgggttgcg acacggtgca gggtttcctg ttcgccaggc cgatgccggc
     3061 ggcggccttc gtcggcttcg tcaaccaatg gaggaacacc accatgaacg ccaatgaacc
     3121 gagcaccagt tgctgcgtgt gctgcaagga aatcccgctc gatgccgcct tcacgccgga
     3181 aggggccgag tacgtggagc atttctgcgg gctggagtgc tatcagcgct tccaggcgcg
     3241 ggccagcact gcgaccgaaa ccagcgtcaa accggacgct tgtgattcgc cgccgtcagg
     3301 ttgaggcata ccctaacctg atgtcagatg ccatgtgtaa attgcgtcag gataggattg
     3361 aattttgaat ttattgacat atctcgttga aggtcataga gtcttccctg acattttgca
     3421 gggaattcca tgactggaca gcgcattggg tatatcaggg tcagcacctt cgaccagaac
     3481 ccggaacggc aactggaagg cgtcaaggtt gatcgcgctt ttagcgacaa ggcatccggc
     3541 aaggatgtca agcgtccgca actggaagcg ctgataagct tcgcccgcac cggcgacacc
     3601 gtggtggtgc atagcatgga tcgcctggcg cgcaatctcg atgatttgcg ccggatcgtg
     3661 caaacgctga cacaacgcgg cgtgcatatc gaattcgtca aggaaggaac acggtgtacc
     3721 tggaacgcgc cacccagggg ttggtcgagg ccggcaagcc ggtggacggc gagctgctgc
     3781 aattcctgtc gccgctgggc tgggagcaca tcaacctaac cggcgattac gtctggcggc
     3841 agagccgcag actggaagac gggaagtttc ggcccttacg gatgcccgga aaaccttagc
     3901 gtacgatttt ttccgaattc tgcgggctcc cctatctcat ctgcgcaagg cagaacgtga
     3961 agacggccgc cctggacctc gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc
     4021 gcgccacggc ccacgagcgg acgccgcagc aggagcgcca gaaggccgcc agagaggccg
     4081 agcgcggccg tgaggcttgg acgctagggc agggcatgaa aaagcccgta gcgggctgct
     4141 acgggcgtct gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt
     4201 gagtgggttg cgctccggca gcggtcctga tcaatcgtca ccctttctcg gtccttcaac
     4261 gttcctgaca acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc
     4321 gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga
     4381 gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc
     4441 ctcaagcacg gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc
     4501 ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg
     4561 tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg
     4621 cctgaagctg cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac
     4681 cgaatgcgta tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt
     4741 gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg
     4801 tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt
     4861 attcggctgc aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc
     4921 aacttatcag tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg
     4981 ccagacgtga aacccaacag acccctgatc gtaattctga gcactgtcgc gctcgacgct
     5041 gtcggcatcg gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg
     5101 aacgacgtca ccgcccacta tggcattctg ctggcgctgt atgcgttgat gcaatttgcc
     5161 tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc
     5221 tcgctggccg gcgctgctgt cgactacgcc atcatggcga cggcgccttt cctttgggtt
     5281 ctctatatcg ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct
     5341 tatattgccg atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc
     5401 tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc
     5461 ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt
     5521 ttccttttgc cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac
     5581 ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc
     5641 ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag
     5701 gatcgctttc actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg
     5761 cattcactcg cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg
     5821 gcactcatgc tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca
     5881 cggggatgga tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg
     5941 gcgctgcaag caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc
     6001 tcactggcgg cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc
     6061 tatgcggctt ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac
     6121 ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat
     6181 cgctgatcgt ggaaacgata ggcctatgcc atgcgggtca aggcgacttc cggcaagcta
     6241 tacgcgccct aggagtgcgg ttggaacgtt ggcccagcca gatactcccg atcacgagca
     6301 ggacgccgat gatttgaagc gcactcagcg tctgatccaa gaacaaccat cctagcaaca
     6361 cggcggtccc cgggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga
     6421 tcccccggaa ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc
     6481 cgagaacatt ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa
     6541 cgagcagaag tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag
     6601 gttgccactt gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg
     6661 ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg
     6721 cagttccgca aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg
     6781 cagagatgaa cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg
     6841 gcaggcggta gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga
     6901 ggatgaagat gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca
     6961 ataaacccgc cggcaacgcc cgcagcatcg cgaccgtcat cggtgagaag ttcggcaggt
     7021 attgggtggt gacaatgtag gtgctgcccc aaatggcagg tgctatcgct gtgaacaata
     7081 aatcgggcgt gcgtaatgac atgcgattac aagacctccg ctacggcgat gggtttttga
     7141 gagttgcagg cgccgcgatg accgcaccct gacttggact ggccctgcct cgcaccaatc
     7201 cacctgcggc tatggccgcg atcatgcgag gcccgaaaat tacacgacct gtacacgtcc
     7261 gccgaacccg gaacgcaaca gagcttcgtg ggcatcccga tccgggtcgt agcagcagtc
     7321 ctggaccaaa cgcacgtcgt agtccgcatc actagcccag gcgacgcttg aaagaacaac
     7381 gccggtggtg cttatcccgg ccatgacaag cgtgcttacg ccgcgcgtcc gaaggtcggc
     7441 gtcgagcgcc gtgccataaa agacgctggc tcgcggacat gcatagaaaa ggtcgccccg
     7501 ttcgatcgca agcccttcga cgggcagacc ggtgcgaaat cgtccgctcg ggagatacgg
     7561 tgagatttgg cgattcgtcg ccggcggcgc atgttcatac tcttcgccga gcgaaaagtt
     7621 ggggaacagc acgggccggc cgcgcaaggc gtgctggcag ccgtgcagac cctgcgtgag
     7681 atgaacgccg acaacctgcg caaggtgccg gccgatgcac ccacggcctt catcaagccg
     7741 cgctggaagc cgctggtgat caccccggaa ggcctcgacc ggaaattcta cgaaatctgc
     7801 gccctgtccg agctgaagaa cgccctgcgc tccggcgaca tctgggtcaa gggctcgcgg
     7861 cagttccgcg acttcgacga ctacctgctg ccggccgaga agttcgccgc actcaagcgc
     7921 gagcaggccc tgcccctggc gatcaacccg aacagcgacc agtacctgga agagcgtttg
     7981 cagctgctgg acgagcagtt ggccaccgtc acccgcctgg ccaaggacaa cgagctgccc
     8041 gatgccatcc tcaccgagtc agggctgaaa atcaccccgc tggatgcggc ggtgccggat
     8101 cgggcgcagg cgctgatcga ccaaaccagc cagttactgc cgcgcatcaa gatcaccgaa
     8161 ctgctgatgg acgtggacga ctggacgggc ttcagccgcc acttcaccca cttgaaggac
     8221 ggggccgagg ccaaagacag gacgttgctg ctgtccgcaa tcctcggtga tgcgatcaac
     8281 ctcgggctga ccaagatggc cgagtcgagc cccggcctga cctacgccaa gctgtcctgg
     8341 ctgcaagcct ggcacatccg cgacgaaacc tattcggcgg ccttggccga gctggtcaac
     8401 caccagtatc gccacgcctt tgccgcccac tggggcgacg gcacgacctc atcctccgat
     8461 ggccagcgct tccgcgcggg tggccggggc gagagcaccg ggcacgtcaa cccgaagtac
     8521 ggtagcgagc cgggacggct gttctatacc catatctccg accagtacgc gccgttcagc
     8581 acccgcgtgg tgaatgtcgg cgtccgcgat tccacctatg tgctcgacgg cctgctgtac
     8641 cacgagtccg acctgcggat cgaggagcac tacaccgaca cggccggctt caccgatcac
     8701 gtctttgccc tgatgcacct gctaggcttc cgcttcgcgc cgcgcatccg cgacctcggc
     8761 gaaaccaagc tgtacgtgcc gcagggcgtg caagcctacc cgacgttgcg cccgctgatc
     8821 ggcggcaccc tgaacatcaa gcacgtgcgt gcccactggg acgacatcct gcgcctggcc
     8881 agctcgatca agcagggcac cgtcaccgcc tcgctgatgc tgcgcaagct cggcagctac
     8941 ccgcgccaga acggactggc cgtggccctg cgcgagctgg gccggatcga gcgcacgctg
     9001 ttcatcctgg actggctgca aagtgttgaa ctgcgccgcc gcgtgcatgc cggcctgaac
     9061 aaaggtgagg cgcgcaactc gctggccagg gcggtgttct tcaaccgcct tggggaaatc
     9121 agggatcgga gcttcgagca gcagcgctac cgggccagcg gcctcaacct ggtgacggcg
     9181 gctatcgtgc tgtggaacac ggtgtacctg gaacgcgcca cccaggggtt ggtcgaggcc
     9241 ggcaagccgg tggacggcga gctgctgcaa ttcctgtcgc cgctgggctg ggagcacatc
     9301 aacctaaccg gcgattacgt ctggcggcag agccgcagac tggaagacgg gaagtttcgg
     9361 cccttacgga tgcccggaaa accttagcgt acgatttttt ccgaattctg cgggctcccc
     9421 gtgcacggca agtgaactcc ggcccctggt cagttcttat cgtcgccgga tagcctcgaa
     9481 acagtgcaat gctgtccaga atacgcgaga cctgaacgcc tgaaatccca aaggcaacag
     9541 tgaccgtcag gcattccttt gtgaaatcat cgacgcaggt aagacacttg atcctgcgac
     9601 cggtggaaag tgcgtccatg acgaaatcca tcgaccaggt cagattgggc gccgccggac
     9661 ggagcagcgg cagacgttct gttgccagcc ctttacgacg tcttctgcgt tttacgccca
     9721 ggccactgag gtgataaagc cggtacacgc gcttatgatt aacatgaagc ccttcacggc
     9781 gcagcaactg ccagatacga cggtagccaa aacgcctgcg ctccagtgcc agctcagtga
     9841 tgcgccctga taaatgcgca tcagcagccg gacggtgagc ctcatagcgg caggtcgaca
     9901 gggataaacc tgtaagcctg caggcacgac gttgcgacag acccttagcc tcgcacatga
     9961 cttccacggc ttcccgcttc tggtctgtcg tcagtacttt cgcccaagag ccacctgaag
    10021 cgcctcttta tccagcatgg cttcggcaag cagcttcttg agtctggcgt tctcttcctc
    10081 aagcgacttc agacgcttaa cttcaggcac ctccataccg ccatacttct tacgccaggt
    10141 gtaaacgtgg catcggaaat ggcatgcttg cggcagagtt cacgggcggg taccccagct
    10201 tcggcttcgc ggagatattg atgatctgtt cgtcggaaaa acgcttcttc atggggatgt
    10261 cctcatgtgg cttatgaaga cattactaac atcggggtgt actaatcaac ggggagcagg
    10321 tcaggctctg tgaataactc aatgaggcct tcgcttacag cggccgcacg cgtccgcgtc
    10381 gctcagataa aagtagctcc catcgcttcg cgtcgggccc cagcggaaga actgcattag
    10441 ttacagcatt caaaagaaaa ttcatcataa aaccggcgcc ggtttgccgg ccaggagccc
    10501 ctccccacca ctgaaaagcg ccgccgcccc cggccgaagg ccgggagtga tgtcgctttt
    10561 aatgatgggt gttgtaacta ctcttcgtca tctctgtgag tagtgtcgcg gtaagttctg
    10621 ggatacacgc tcgtaagcgg ccctaacggc ccgctaacgc ggagatacgc cccgactgcg
    10681 gctgtcgcct tgccgggacc actccgaccg cgtacagagg catctgagcc gtttttagcg
    10741 ggtatagctt tgcagggaag ggggtgggtg aggcagaacc tgtcaaaaca gcagccggct
    10801 gcgccgggcg acggcgccat ttacccgcag tactccattc aacttcagct ggcgcctcca
    10861 tgccgctgac gcggcattat ctatacttta agtctggcca ctggaggtaa ccatgtgcgg
    10921 acgtttttct cagtctatga cgcgggaaga gtatctttct ctcctcgcca atgaagccga
    10981 tcgaaatatt gcctac
//
LOCUS       DAZFYC010000151        10974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFYC010000151 DAZFYC010000000
VERSION     DAZFYC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.151"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..104)
                     /locus_tag="ACHKW5_004484"
     tRNA            complement(20..104)
                     /locus_tag="ACHKW5_004484"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Tyr,seq:gta)
     gene            complement(264..1106)
                     /gene="purU"
                     /locus_tag="ACHKW5_004485"
     CDS             complement(264..1106)
                     /gene="purU"
                     /locus_tag="ACHKW5_004485"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO7513723.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1156..1614)
                     /gene="ychJ"
                     /locus_tag="ACHKW5_004486"
     CDS             complement(1156..1614)
                     /gene="ychJ"
                     /locus_tag="ACHKW5_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO7513724.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1727..2632
                     /gene="rssA"
                     /locus_tag="ACHKW5_004487"
     CDS             1727..2632
                     /gene="rssA"
                     /locus_tag="ACHKW5_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO7513725.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2724..3737
                     /gene="rssB"
                     /locus_tag="ACHKW5_004488"
     CDS             2724..3737
                     /gene="rssB"
                     /locus_tag="ACHKW5_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO7513726.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3939..4847
                     /gene="galU"
                     /locus_tag="ACHKW5_004489"
     CDS             3939..4847
                     /gene="galU"
                     /locus_tag="ACHKW5_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO7513727.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4991..5404)
                     /gene="hns"
                     /locus_tag="ACHKW5_004490"
     CDS             complement(4991..5404)
                     /gene="hns"
                     /locus_tag="ACHKW5_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO7513728.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6009..6626
                     /gene="tdk"
                     /locus_tag="ACHKW5_004491"
     CDS             6009..6626
                     /gene="tdk"
                     /locus_tag="ACHKW5_004491"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO7513729.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(6928..9603)
                     /gene="adhE"
                     /locus_tag="ACHKW5_004492"
                     /gene_synonym="adhC"
     CDS             complement(6928..9603)
                     /gene="adhE"
                     /locus_tag="ACHKW5_004492"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO7513730.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMESYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            9996..10049
                     /gene="ychE"
                     /locus_tag="ACHKW5_004493"
     CDS             9996..10049
                     /gene="ychE"
                     /locus_tag="ACHKW5_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO7513731.1"
                     /translation="MLPKGVLSARLFNLIYG"
     gene            10080..10727
                     /gene="ychE"
                     /locus_tag="ACHKW5_004494"
     CDS             10080..10727
                     /gene="ychE"
                     /locus_tag="ACHKW5_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO7513732.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         2823 a         2449 c         2693 g         3009 t
ORIGIN      
        1 acggactttt gaaaagtgat ggtggtgggg gaaggattcg aaccttcgaa gtcgatgacg
       61 gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccacgggg taatgctttt
      121 tactggcctg ctcccttatc gggaagcggg gcgcatcata tcaaatgacg cgccgctgta
      181 aagtgttacg ttgagaaaaa tgaactggtt gcgtaatttt catccgtaac ggattaaagg
      241 taaccagtta tttttgctgg cgattaaaga ataatcgtcc gattaccgta aacaaagacg
      301 cgctgtgcca gtactttgta tagcgcacga cttaagacgt ttttctcgac gtcacgacct
      361 gcgcgcatca tatcttcagc tgtgtaggta tgatcgacat gaataacgtc ctgcatgatg
      421 attgggcctt cgtccagatt gtcattcaca tagtgagcgg ttgcgccaat aatcttcaca
      481 ccacgttcat aggcctggtg ataaggacgt gcgccgataa acgcaggcag gaatgaatgg
      541 tgaatattga tgattttatt cgggaagcgt gccacaaatt ccggcgttaa tacccgcata
      601 tacttcgcca gcaccacata gtcaggttga taagcatcaa tggcgtccgc catcttttga
      661 tcgtgctcgt tgcggcttaa cccttcatgg cttaccagct caaacggaat atcaaaacgc
      721 tcaaccagag aacgtaaagt atcgtggtta ccaataactg ccgcgatttc gacatccagg
      781 ccgccgtaat tggctttcat caacaaatcg ccaaggcaat gcgcttcttt agtgaccaga
      841 atcactatcc ggcgacgacc ggcaggattc agctcacgca cggagccttc tggcaatgcg
      901 ctatcgagat ccgccagcag ggtggaatca ttaaaaatcc cttccagttc cgtgcgcata
      961 aaaaagcgcc cggtacggtg atcaacaaat tcattgttct gtacgatatt taactcgtgc
     1021 ttgtagcaaa tattggtaat acgtgcgatc agaccttttt ggtccggaca aatagtacgc
     1081 agaactttac gttggagtga atgcattgct ggaaaaacct tgttgagagt gtttgctaaa
     1141 ccgtaccgtc aaccattatt ggccgcagca ctttttaaat tttttacctg aaccacaagg
     1201 gcagggatcg ttgcgaccaa actgcggacg tgtaccgtca atatagtacc attgaccgtt
     1261 ttctttcaaa aatcgcgaac gttcaattat tgcacctgtt ttaccacctt cggtaaatcg
     1321 agcaacaaaa ctgacaaatc cgatattgtc ggcgtcctgc caacaatgtt caaatacggt
     1381 caatcccaac cattcagtat gcgcaaatcc ggccatcaat tcggcacgta acgccgctgc
     1441 tccacaagag ggatgccagg tctttattaa ataatctgcg tcttgcatca caaaagcgca
     1501 gtaacgcgaa cgcatgagat gttctggatc aggtgcaacc ttttcaccag acacataagg
     1561 gtggcaacat aggctatact cgacagcact accacaggga caaagctgag acacaaataa
     1621 tctccctgga aacaataacg gcgtattaac cgcctgagta gcactatgtt aaccgagcag
     1681 tagcgatgtg gctacgattg cattccaggg gaatcttgcg ggaataatga gaaagataaa
     1741 aatagggctg gcgctgggat ctggcgcggc gagaggttgg tcgcatattg gcgttattaa
     1801 tgcgctaaaa aaagtgggta ttgaaattga tatcgttgca ggatgttcaa ttggttcgct
     1861 ggtgggcgct gcctatgcat gcgatcgatt atctgcgctg gaagattggg tgacctcttt
     1921 cagttattgg gatgttttac gcctgatgga tctctcctgg cagcgcggtg ggttactgcg
     1981 cggcgagcgt gtcttcaatc aataccgcga aataatgccg gaaacagaga tcgaaaattg
     2041 ttcccgtcgc tttgcggctg ttgccaccaa tttaagtacg ggacgtgaat tatggtttac
     2101 tgaaggcgat ctccatcttg ctattcgcgc atcatgcagt attccaggac tcatggcacc
     2161 tgttgcacat aacggctact ggctggttga tggagcagtc gttaacccaa ttcctatttc
     2221 cctcacgcgt gcattggggg ctgatattgt gatagcggtt gacctgcagc acgatgctca
     2281 tttgatgcaa caagatttgc tctcctttaa tgtcagtgaa gaaaatagcg agaatggtga
     2341 ttctctgccg tggcatgcgc gtctgaaaga aaggttaggc agcataacga cacgtcgggc
     2401 ggtgacagcg ccaacggcaa cagagattat gaccacttct atccaggtgc tggagaaccg
     2461 ccttaaaagg aaccgcatgg caggtgatcc gcccgatatt ctgattcaac ctgtttgccc
     2521 gcaaatatct acgcttgatt tccatcgcgc gcacgctgcc attgcggccg gacagctggc
     2581 agtggaaagg aaaatggacg aacttttgcc gttggtacgc accaacattt gaccagaatt
     2641 tttatctaca cttaagttaa ttctgacagg cgcaggtggc aatagcatgc cactattgag
     2701 taaagccagt caggggagag aacatgacgc agccattggt cggaaaacag attctcattg
     2761 ttgaagatga gcaggtattt cgctcgcttc tggattcatg gttttcctca ttgggagcga
     2821 caacggtact ggcggctgat ggggtggatg cccttgagtt gctgggaggt ttcactccag
     2881 acctgatgat atgtgatatc gcgatgccac gaatgaacgg gcttaaactg ctggagcata
     2941 tacgtaacag aggcgaccag accccagttc tggtgatatc tgccactgaa aatatggcag
     3001 atattgccaa agcgttacgt ctgggcgttg aagatgtttt gctgaaacca gttaaagatc
     3061 tgaatcgctt gcgcgagatg gtttttgcct gtctctatcc cagcatgttt aattcgcgcg
     3121 ttgaggaaga ggaaaggctt tttcgcgact gggatgcaat ggttgataac cctgccgcag
     3181 cggcgaaatt attacaggaa ctacaaccgc cggttcagca ggtgatttcc cattgccggg
     3241 ttaattatcg tcaattggtt gccgcggaca aacccggcct ggtgcttgat attgccgcac
     3301 tttcggaaaa cgatctggca ttttattgcc ttgatgtcac tcgagctgga cataatggcg
     3361 tacttgctgc cttgttatta cgcgcattgt ttaacggatt attacaggaa cagcttgcac
     3421 accaaaatca acggttgcca gagttgggcg cgttattgaa gcaggtaaac catttactcc
     3481 gtcaggccaa tctgccgggg cagtttccgc tattagttgg ctattatcat cgcgaactga
     3541 aaaatctcat tctggtttct gcgggtctga atgcgacgtt aaataccggc gaacaccagg
     3601 tgcaaatcag taatggtgtt ccgttaggca ctttaggtaa cgcttatttg aatcaattga
     3661 gccagcgatg cgatgcctgg caatgccaaa tatggggaac cggtggtcga ctgcgcttga
     3721 tgttgtctgc agaatgagca aacgataacg cgggctaaat ttgcattacc tgctaatgtc
     3781 ggctggtggt actatcgtcg ccattcgtat aagtaattgt cttaattatg ctaactcgcc
     3841 tccttttcag aacttagccc cttcggggtg ctgatatact gggatgcgat acagaaatat
     3901 gaacacgttc aaaacacgaa cagtccagga gaatttaaat ggctgccatt aatacgaaag
     3961 tcaaaaaagc cgttatcccc gttgcgggat taggaaccag gatgttgccg gcgacgaaag
     4021 ccatcccgaa agagatgctg ccacttgtcg ataagccatt aattcaatac gtcgtgaatg
     4081 aatgtattgc ggctggcatt actgaaattg tgctggttac tcactcatct aaaaactcta
     4141 ttgaaaacca ctttgatacc agttttgaac tggaagcaat gctggaaaaa cgtgtaaaac
     4201 gtcaactgct tgatgaagtg cagtctattt gtccgccgca cgtaactatt atgcaagttc
     4261 gtcaggggct ggcgaaaggc ctgggacacg cggtattgtg tgctcacccg gtagtgggtg
     4321 atgaaccggt agctgttatt ttgcctgatg ttattctgga tgaatatgaa tccgatttgt
     4381 cacaggataa cctggcagag atgatccgcc gctttgatga aacgggtcat agccagatca
     4441 tggttgaacc ggttgctgat gtgaccgcat atggcgttgt ggattgcaaa ggcgttgaat
     4501 tagcgccggg tgaaagcgta ccgatggttg gtgtggtaga aaaaccgaaa gcggatgttg
     4561 cgccgtctaa tctcgctatt gtgggtcgtt acgtacttag cgcggatatt tggccgttgc
     4621 tggcaaaaac ccctccggga gctggtgatg aaattcagct caccgacgca attgatatgc
     4681 tgatcgaaaa agaaacggtg gaagcctatc atatgaaagg gaagagccat gactgcggta
     4741 ataaattagg ttacatgcag gccttcgttg aatacggtat tcgtcataac acccttggca
     4801 cggaatttaa agcctggctt gaagaagaga tgggcattaa gaagtaacat ccgtatcggt
     4861 gttatccacg aaacggcgtt gagcaatcga cgccgttttt ttatagctta ttcttattaa
     4921 attgtcttaa accggacaat aaaaaatccc gccgctggcg ggattttaag caagtgcaat
     4981 ctacaaaaga ttattgcttg atcaggaaat cgtcgaggga tttaccttgc tcatccattg
     5041 cttttttgat tacagctgga gtacggcctt ggccagtcca ggttttagtt tcgccgtttt
     5101 cgtcaacgta gctatatttt gccggacgct gagcacgttt agctttggtg ccagatttaa
     5161 cggcagcaag gctattcagc agttcgttcg ggtcaatacc gtcagcgatc agcatttcgc
     5221 gatattgctg cagtttacga gtgcgctctt caacttcagc agcagccgcg ctttcttctt
     5281 cgcgacgttc gttaacaaca acttctaatt tttccagcat ttcttccagc gtttcaagtg
     5341 tacattctct tgcctgcgca cgaagagtac ggatgttgtt cagaatttta agtgcttcgc
     5401 tcattgtagt aatctcaaac ttatattggg gtggtttgtt gaggtaataa tagagcctta
     5461 aattcagttg tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt attttgtgcc
     5521 gccaataaat atcttttcat aaaattagcc agaaaagacg cggcatatag ccctatttac
     5581 accgatgatt tcgcagcacg tgaggttaaa acttcttgat tcatgtcaca ttttatgggg
     5641 agattatcgt aggctgacga cctttcagtc ttctgtatta gttgtgttta cgagaattcc
     5701 ctattaagcg aatgatgaaa agtagaacag tcgcaataag agcatggact tagtattgca
     5761 ctatctcctg gaggtaaaca gagggctatt acttgcgcaa caggttaaag attgtgaata
     5821 gttaccagca gtcatttacc cgcttataac aagcgaggca gttgtaatga tagctcagaa
     5881 ggattatgca aggcttcgta agggagaacg catataccca cttctgtgca tactgttgag
     5941 ctgaaaaact gacgaattat gataaactcc agccaacttt atttcatatc attgagggcc
     6001 tgtggctgat ggcacagcta tatttctact attccgcaat gaatgcgggt aagtctacag
     6061 cattgttgca atcttcatac aattaccagg aacgcggcat gcgcactgtc gtatatacgg
     6121 cagaaattga tgatcgcttt ggtgccggga aagtcagttc gcgtataggt ttgtcatcgc
     6181 ctgcaaaatt atttaaccaa aattcatcat tatttgatga gattcgtgcg gaacatgaac
     6241 agcaggcaat tcattgcgta ctggttgatg aatgccagtt tttaaccaga caacaagtat
     6301 atgaattatc ggaggttgtc gatcaactcg atatacccgt actttgttat ggtttacgta
     6361 ccgattttcg aggtgaatta tttattggca gccaatactt actggcatgg tccgacaaac
     6421 tggttgaatt aaaaaccatc tgtttttgtg gccgtaaagc aagcatggtg ctgcgtcttg
     6481 atcaagcagg cagaccttat aacgaaggtg agcaggtggt aattggtggt aatgaacgat
     6541 acgtttctgt atgccgtaaa cactataaag aggcgttaca agtcggctca ttaacggcta
     6601 ttcaggaaag gcatcgccac gattaataag aatttcttta ctgacagggt gagcagggca
     6661 cttttatcct gtcagttcgt tttacgcact tcttccgggc tatataccct tctcggcagt
     6721 tttttaacgc cgctatacgc ctcacagggc tcttaagcac cgacgttgac ttgtgaccta
     6781 taaagtacaa tatccctgtg tttaggcgtt atacatcgtc gcaaatatga tgaaggctaa
     6841 tgctgtcggt ttatggaaaa gttgctttgg gtaaacaaaa aaacggcccc agaaggggcc
     6901 gtttatattg ccagacagcg ctactgatta agcggatttt ttcgcttttt tctcagcttt
     6961 agccggagcg gcttcttttt tcgctgcagt ttcaccttct acataatcac gaccgtagta
     7021 ggtatccagc aggatctgtt tcagctcgga gatcagcggg taacgcgggt tagcgccggt
     7081 gcactggtca tcgaacgcat cttcagacag tttatccacg ttcgccagga agtctgcttc
     7141 ctgaacgcca gcttcacgga tagatttcgg aatacccagt tcagctttca gcgtttccag
     7201 ccatgccagc agtttctcga tcttagcagc agtacggtcg cccggtgcgc tcagacccaa
     7261 gtggtcggca atttcagcat aacgacggcg agcctgcgga cggtcatact ggctgaatgc
     7321 agtctgcttg gtcgggttgt cgttcgcatt gtagcgaata acgttacaaa tcagcagggc
     7381 gtttgccaga ccgtgcggaa tatggaactg ggaacccagt ttgtgcgcca ttgagtgaca
     7441 tacacccagg aaggcgttcg caaacgcgat acccgcgata gtcgctgcac tgtgaacacg
     7501 ttcacgcgct accggatttt tagacccttc gtggtaggac gctggcagat attctttcag
     7561 cagtttcagt gcctgcagag cctgaccatc agagaactca gatgccagta cagaaacata
     7621 agattccatg gcgtgagtta ctgcgtccag accaccgaaa gcacacaggg acttcggcat
     7681 gtccataacc aggttggcgt cgacaatcgc catatccgga gtcagcgcat agtctgccag
     7741 cggatatttc tgaccagtag cgtcgtcagt tacaaccgca aacggagtga cttcagaacc
     7801 tgtaccagaa gtggtggtga cagcgatcat tttcgctttc acgcccattt tcgggaactt
     7861 gtagatacgt ttacggatat ccataaagcg cagcgccagc tcttcgaagt gagtttccgg
     7921 atgttcgtac ataacccaca tgatcttcgc ggcgtccatc ggggaaccac cacccagcgc
     7981 gataatcacg tctggtttga aggagtttgc cagttctgca cctttacgaa cgatgctcag
     8041 ggtcgggtcc gcttctactt cgaagaagac ttcagtttca acgcctgctg ctttcagtac
     8101 ggaagtgatc tgatcagcat aaccattgtt gaacaggaag cggtcagtca cgatgagcgc
     8161 acgtttgtgg ccatcagtaa tcacttcatc cagcgcgatt ggcagggagc cacggcggaa
     8221 gtagatagat ttcggaagtt tgtgccacaa catgttttca gctcgcttag caacggtttt
     8281 cttgttgatc aggtgtttcg gaccaacgtt ttcagagatg gagttaccac cccaagaacc
     8341 acaacccaga gtcagggaag gtgcgagttt gaagttatac aggtcaccga taccaccctg
     8401 agacgctggg gtgttaatca ggatacgagc cgttttcatt ttttgaccga agtaagaaac
     8461 gcgagccggt tggttatcct ggtcagtgta caggcaagag gtatgaccga taccgcccat
     8521 agcaaccagt ttctctgctt tttctaccgc gtcttcgaaa tctttagcgc ggtacattgc
     8581 cagagtcggg gacagttttt catgtgcgaa cggttcgctt tcatcaacaa cggtcacttc
     8641 accgatcaga atcttggtgt tttctggtac agagaagcct gccagttcag ctattttata
     8701 ggctggctga ccaacgatag ccgcgttcag cgcaccgttt ttcaggataa catcctgaac
     8761 agctttcagc tctttaccct gcaacagata gccgccgtgg gttgcaaaac gttcacgtac
     8821 agcgtcataa acagagtcaa caacaacaac agactgttca gaagcacaga ttacgccgtt
     8881 gtcgaaggtt ttggacatca gtacagatgc aactgcacgt ttgatatcag cagtttcatc
     8941 gataacaact ggagtgttgc ccgcgcctac accgatagct ggtttaccgg agctgtatgc
     9001 ggctttaacc atgcccggac caccagtcgc gaggatcagg ttgatgtctg ggtggtgcat
     9061 cagtgcgtta gacagttcaa cagaaggttg atcgatccag ccgatcagat ctttcggagc
     9121 accggcagcg atagcagcct gcagaacgat atcagccgct ttgttggtgg catcttttgc
     9181 acgcgggtgc ggggagaaga taatggcgtt acgggtcttc agactgatca gcgatttgaa
     9241 gatagcagtt gaagtcgggt tagtggtcgg aacgataccg caaataatac cgattggttc
     9301 agcgatagtg atggtaccaa aagtgtcgtc ttcagacaga acaccacagg ttttttcatc
     9361 tttataggcg ttgtagatat attcagaagc aaagtggttt ttgatcactt tatcttcgac
     9421 gatacccatg ccggattcgg caacggccat tttcgcgagt gggattcgag catctgcagc
     9481 agccagagcg gcggcgcgga agattttgtc tacttgctct tgagtgaaac tggcatattc
     9541 acgctgggct ttttttacac gctctacgag tgcgttaagt tcagcgacat tagtaacagc
     9601 cataatgctc tcctgataat gttaaacttt tttagtaaat catctgctcg aatacgagag
     9661 tatagtcagt gcggtgatga tttgcttaac ctatgaaaat caaaagctta ctcgcgctca
     9721 cactcactgt gatttactaa aagagtttaa acattagagt tattatctct aatgcgtcac
     9781 ttccaggtgg cgtaagcaag attactcact tctgggtact gattacgtga tccaaatcaa
     9841 atttttgcaa agctgacacc tttcagcatc gcttttcgcc attatagcta acagttaata
     9901 aattgtagta tgatttggtg gctacattag catgttttgc acaactagat aacaataacg
     9961 aatgatagca attttaagta gttaggaggt gaaaaatgct gccaaaaggc gtattgtcag
    10021 cgcgtctttt caaccttatt tatggctaac attatccggc ttttgcttcg gagctaaccg
    10081 tgattcagac cttttttgat tttcccgttt acttcaaatt tttcatcggg ttatttgcgc
    10141 tggtcaaccc ggtagggatt attcccgtct ttatcagcat gaccagttat cagacagcgg
    10201 cagcgcgaaa caaaactaac cttacagcca acctgtctgt ggccattatc ttgtggatct
    10261 cgctttttct cggcgacacg attctgcaac tttttggtat atcaattgat tcgttccgta
    10321 tcgccggggg catcctggtg gtgacgatag cgatgtcgat gatcagcggc aagcttggcg
    10381 aggataaaca gaacaagcaa gaaaaatcag aaaccgcggt acgtgaaagc attggtgtgg
    10441 tgccattggc gttgccgttg atggcggggc caggggcgat cagttctacc atcgtctggg
    10501 gtacgcgtta tcacagcatt agctatctgt ttggtttctt tgtggctatt gcattgttcg
    10561 ctttatgttg ctggggattg ttccgcatgg caccgtggct ggtacgggtt ttacgccaga
    10621 ccggcatcaa cgtgattacg cgtattatgg ggctattgct gatggcattg gggattgaat
    10681 ttatcgttac tggtattaag gggattttcc ccggcctgct taattaattc ctttcaaatg
    10741 aaacggagct gccatgctcc gtttacttcg tcattatttt tactttgttc ccgcgcagtt
    10801 atcaaaagca aaaggaatag gtaaaaatat tcttctcaaa ttacagttag ttataaggat
    10861 ttccttaact gcttctcctc accatcatgt tattttcgcc acatcataat cctgggcttg
    10921 ctgaagaata attgaaatga tattagtaat tccactgcct ttggtagagg aaag
//
LOCUS       DAZFYC010000152        10919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFYC010000152 DAZFYC010000000
VERSION     DAZFYC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.152"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            256..1398
                     /gene="yghO"
                     /locus_tag="ACHKW5_004495"
     CDS             256..1398
                     /gene="yghO"
                     /locus_tag="ACHKW5_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO7513733.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGVEPYKRYRLYEKQI"
     gene            complement(1405..2169)
                     /gene="glcC"
                     /locus_tag="ACHKW5_004496"
     CDS             complement(1405..2169)
                     /gene="glcC"
                     /locus_tag="ACHKW5_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO7513734.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTNLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2420..3919
                     /gene="glcD"
                     /locus_tag="ACHKW5_004497"
     CDS             2420..3919
                     /gene="glcD"
                     /locus_tag="ACHKW5_004497"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO7513735.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3919..4971
                     /gene="glcE"
                     /locus_tag="ACHKW5_004498"
     CDS             3919..4971
                     /gene="glcE"
                     /locus_tag="ACHKW5_004498"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO7513736.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEL
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4982..6205
                     /gene="glcF"
                     /locus_tag="ACHKW5_004499"
     CDS             4982..6205
                     /gene="glcF"
                     /locus_tag="ACHKW5_004499"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO7513737.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6210..6614
                     /locus_tag="ACHKW5_004500"
     CDS             6210..6614
                     /locus_tag="ACHKW5_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO7513738.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6636..8807
                     /gene="glcB"
                     /locus_tag="ACHKW5_004501"
     CDS             6636..8807
                     /gene="glcB"
                     /locus_tag="ACHKW5_004501"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO7513739.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMDKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9162..10844
                     /gene="glcA"
                     /locus_tag="ACHKW5_004502"
     CDS             9162..10844
                     /gene="glcA"
                     /locus_tag="ACHKW5_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO7513740.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2543 a         2729 c         3057 g         2590 t
ORIGIN      
        1 taaataatga gtttaagttt tttgttttta ttgtgaatga tatttgattt atgaatatat
       61 taagaagaag ccttgtccgg tcgccttaaa tcgacaaaag gcattgaggc gcttctgtgt
      121 agtctctaac cttatcataa tcattaataa atactattta ttctttgttt gtattcattg
      181 ggttgattat taaggtgtcg ttcgttgctg ttgcaacctg tttttcaccg ttaattattt
      241 gttatccaca gagatatgga atgcgatctg ctgatgatta aaattgaaaa agtaattaat
      301 aaaaatgacc ttaaggcatt tatcgctttt ccgtcatcac tttatcccga cgatccaaac
      361 tggatacccc ctttattcat agaacgcaac gagcatttgt ctgcgaaaaa tccggggacg
      421 gaccatatca tctggcaggc gtgggtggca aaaaaagcgg ggcagatagt tgggcgtatt
      481 accgcgcaaa tagatacctt gcaccgtgag cgttacggtg aagataccgg tcatttcggc
      541 atgattgacg ccattgatga tccgcaggtt tttgccgcgt tgtttggcgc agcggaagcg
      601 tggttgaagt cacaaggtgc aagtaagatc agcggtcctt tcagcctgaa tatcaatcag
      661 gaaagcggat tactgattga aggttttgac acaccaccct gtgcgatgat gccacacggc
      721 aaaccgtggt atgccgcgca tattgaacaa ctgggttatc acaaaggcat tgatttactg
      781 gcgtggtgga tgcagcgaac cgatctcact ttctctccgg cgctaaaaaa actgatggat
      841 caggtgcgca aaaaggtgac cattcgctgc atcaatcgtc agcggtttgc cgaagaaatg
      901 cagatcctgc gtgagatttt caactctggc tggcagcaca actggggatt tgtgccgttt
      961 accgaacatg aattcgcgac catgggcgat caacttaaat atctggtgcc ggacgatatg
     1021 atctatatcg ctgagattga ttctgcaccc tgcgcgttta ttgtcggctt gccgaacatc
     1081 aacgaggcga ttgccgatct gaacggatcg ctctttccct tcggctgggc aaaattgctg
     1141 tggcgtttga aagtcagcgg tgtgcgaact gcgcgagtac cgctgatggg cgtgcgcgac
     1201 gagtatcagt tcagccgcat cggcccggtg attgcgctgt tattgattga agccttacgc
     1261 gatccgtttg cccgccggaa gatcgatgcg ctggagatgt catggatcct cgaaaccaat
     1321 acaggcatga ataacatgct ggaacgcatt ggggtggagc cgtacaagcg ttaccgattg
     1381 tatgaaaagc agatttaata ccagctaact caggttcatc tccagcggca ccgccgagcg
     1441 aatcaaatgg tggccttcca gctcgatatc gtggagattc tttttcacgg tccgcacatg
     1501 atcgcgtgct gcgcgctggg cgacgtgcgg cagccgctgc aacaccgcgt tgtagatcct
     1561 cgcatgctgg cgatcgatct gctttttttg ctgtggtcga tggtagagat tatttacgct
     1621 ggcaaacact gaattaaaca tcagattggt caatgattgc agcgtaaaca ccagcacctg
     1681 attgtgagaa gcctgacaaa tggcgagatg gaaagcgtga tccaactgcg catgttcgat
     1741 cagcgaaatc tctttgttgt tctcactggc ggcgagcatt ttttcataac agcgggttat
     1801 cacaacaaaa tcagcctgcg ttcccagcgt tgccgccagc cttgccgatt cgccctccag
     1861 taatgcgcga acgtcgagca gatcgtacag cgttcgcggc tgcgtactga acagatggat
     1921 cagcgggctg gtgtcctgca cccgattaag ccgtgcgaca cgagaatcgc gaccctgcgc
     1981 cgtttcaata atcccgcgcc cgcgcagcac ggtcagccct tcacgcagtg cggagcgtga
     2041 gaagccgagc ttttcacaca gtcgacgttc cgagggaagt ggctgaccga ccttcagtac
     2101 gccgtcgata attaaccgtt cgatactctc tgcaaccact tcgcaaatag ggcgacgttc
     2161 atctttcatt cccggacctc gtgcacaggt aggaccaatt tttctgttct taactcgcaa
     2221 aacacgcaca tcacgtaagt gattatgtta catcaattta acattgagtt aaccaaggca
     2281 aggtcacaga gctggaaaaa aaatggtctg accggtagct aaagagatag acgaaaacga
     2341 aaagcccgct taataactgt tcacagaagc agcgcgcaaa aatcagctgc cacacaacac
     2401 aacaaagcga agcctactca tgagcatctt gtacgaagag cgtcttgatg gcgctttacc
     2461 cgatgtcgac cgcacatcgg tactgatggc actgcgtgag catgtccctg gacttgagat
     2521 cctgcatacc gatgaggaga tcattcctta cgagtgtgac gggttgagcg cgtatcgcac
     2581 gcgtccatta ctggttgttc tgcctaagca aatggaacag gtgacagcga ttcttgctgt
     2641 ctgccatcgc ctgcgtgtac cggtggtgac ccgtggtgca ggcaccgggc tttctggtgg
     2701 cgcgctgccg ctggaaaaag gtgtgttgtt ggtgatggcg cgctttaaag agatcctcga
     2761 cattaacccc gttggtcgcc gcgcgcgcgt gcagccaggc gtgcgtaacc tggcgatctc
     2821 ccaggccgtt gcaccgcata atctctacta cgcaccggac ccttcctcac aaatcgcctg
     2881 ttccattggc ggcaacgtgg cagaaaatgc cggcggcgtc cactgcctga aatatggtct
     2941 gaccgtacat aacctgctga aaattgaagt gcaaacgctg gacggcgagg cgctgacgct
     3001 gggatcggac gcgctggatt cacctggttt tgacctgctg gcgctgttca ccggatcgga
     3061 aggtatgctc ggcgtgacca ccgaagtgac ggtaaaactg ctgccgaagc cgcccgtggc
     3121 gcgggttctg ttagccagct ttgactcggt agaaaaagcc ggacttgcag ttggtgacat
     3181 catcgccaat ggcattatcc ccggcgggct ggagatgatg gataacctgt cgatccgcgc
     3241 ggcggaagat tttattcatg ccggttatcc cgtcgacgcc gaagcaattt tgttatgcga
     3301 gctggacggc gtggagtctg acgtacagga agactgcgag cgggttaacg acatcttgtt
     3361 gaaagcgggc gcgactgacg tccgtctggc acaggacgaa gcagagcgcg tacgtttctg
     3421 ggccggtcgc aaaaatgcgt tcccggcggt aggacgtatc tccccggatt actactgcat
     3481 ggatggcacc atcccgcgtc gcgccctgcc tggcgtactg gaaggcattg cccgtttatc
     3541 gcagcaatat gatttacgtg ttgccaacgt ctttcatgcc ggagatggca acatgcaccc
     3601 gttaatcctt ttcgatgcca acgaacccgg tgaatttgcc cgcgcggaag agctgggtgg
     3661 gaagatcctc gaactctgcg ttgaagttgg cggcagcatc agtggggaac atggcatcgg
     3721 gcgagaaaaa atcaatcaaa tgtgcgccca gttcaacagc gatgaaatca cgaccttcca
     3781 tgcggtcaag gcggcgtttg accccgatgg tttgctgaac cctgggaaaa acattcccac
     3841 gctacaccgc tgtgctgaat ttggtgccat gcatgtgcat cacggtcatt tacctttccc
     3901 tgaactggag cgtttctgat gctacgcgag tgtgattaca gccaggcgct gctggagcag
     3961 gtgaatcagg caattagcga taaaacgccg ctggtgattc agggcagcaa tagcaaagcc
     4021 tttttaggtc gccctgtcac cgggcaaacg ctggatgtgc gttgtcatcg cggcattgtt
     4081 aattacgacc cgaccgagct ggtgataacc gcgcgtgccg gaacgccgct ggtggcgatt
     4141 gaagcggcgc tggagagcgc cgggcaaatg ctcccctgcg agccgccgca ttatggtgaa
     4201 gaagccacct ggggcgggat ggtcgcctgc gggctggcgg ggccgcgtcg cccgtggagc
     4261 ggttcggtcc gcgattttgt cctcggcacg cgcatcatta ccggcgctgg aaaacatctg
     4321 cgttttggtg gcgaagtgat gaaaaacgtt gccggatacg atctctcacg gttaatggcc
     4381 ggaagctacg gttgtcttgg cgtgctcact gaaatctcga tgaaagtgtt accgcgaccg
     4441 cgcgcctccc tgagcctgcg tcgggaaatc agcctgcaag aagccatgaa cgaaatcgcc
     4501 cagtggcaac tccagccgct acccattagt ggcttatgtt acttcgacaa tgcgttgtgg
     4561 atccgccttg agggcggcga aggatcggta aaagcggcgc gtgaactgct ggggggcgaa
     4621 gaggttgcgg gtcagttctg gcagcaattg cgtgaacaac aactgccgtt cttctcgtta
     4681 ccaggtacct tatggcgcat ttcattaccc agcgatgcgc cgatgatgga tttacccggc
     4741 gagctactga tcgactgggg cggagcgtta cgctggctga aatcgacagc cgaggacaat
     4801 caaatccatc gcatcgcccg caacgctggc ggtcatgcga cccgctttag tgccggagat
     4861 ggtggctttg ccccgctatc ggctccttta ttccgctatc accagcagct taaacagcag
     4921 ctcgaccctt gcggcgtgtt taaccccggt cgcatgtacg cggaactttg aggagcaggc
     4981 tatgcaaacc caattaactg aagagatgcg gcagaacgcg cgcgcgctgg aagccgacag
     5041 catcctgcgc gcctgtgttc actgcggatt ttgtaccgca acctgcccaa cctatcagct
     5101 tctgggcgat gaactggacg ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga
     5161 aggcaacgaa gtcacgctta aaacacagga gcatctcgat cgctgcctca cttgccgcaa
     5221 ttgtgaaacc acctgtcctt ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga
     5281 tattgtcgag cagaaagtaa aacgcccact gccggagcga atactgcgcg aaggattgcg
     5341 ccaggtagtg ccgcgtccgg cggtcttccg tgcgctgacg caggtagggc tggtgctgcg
     5401 accgttttta ccggaacagg tcagagcaaa actgcctgct gaaacggtga aagctaaacc
     5461 gcgtccgccg ctgcgccata agcgtcgggt tttaatgctg gaaggctgcg cccagcctac
     5521 gctttcgccc aacaccaacg cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt
     5581 catgtcagct aacgaagcag gctgttgtgg cgcggtggac tatcatctta atgcgcagga
     5641 gaaagggctg gcacgggcgc gcaataatat tgatgcctgg tggcccgcga ttgaagcagg
     5701 tgccgaggca attttgcaaa ccgccagcgg ttgcggcgcg tttgtcaaag agtacgggca
     5761 gatgctgaaa aacgatgcgt tatatgccga taaagcgcgt caagtcagtg aactggcggt
     5821 cgatttagtc gagcttctgc gcgaggaacc gctggaaaaa ctggcaattc gcggcgataa
     5881 aaaactggcc ttccactgcc cgtgtaccct gcaacatgcg caaaagctga acggcgaagt
     5941 ggaaaaagtg ttgcttcgtc ttggatttac cttaacggac gttcccgaca gccatctgtg
     6001 ctgcggttca gcgggaacat atgcgttaac gcatcccgat ctggcacgcc agctgcggga
     6061 taacaaaatg aatgcgctgg aaagcggcaa accggaaatg atcgtcaccg ccaacattgg
     6121 ttgccagacg catctggcga gcgccggtcg tacctctgtg cgtcactgga ttgaaattgt
     6181 agaacaagcc cttgaaaagg aataaaaaaa tgaaaactaa agtcattctt agccagcaaa
     6241 tggcgagtgc aattattgcc gcaggtcagg aagaggcgca gaaaaataac tggtctgttt
     6301 ccattgctgt tgccgatgac ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg
     6361 cgccgattgc ggcttacatc tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg
     6421 aaactaaggg ctatgaagag atggtgaaca acggacgtac cgcgttcgtg actgcgccgt
     6481 tactgacgtc gctggaaggt ggcgtaccgg ttgttgttga tgggcaaatt attggtgccg
     6541 tgggcgtttc tggtttaacc ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg
     6601 tgttggcgaa ataagcgaaa acgaggagat aaacaatgag tcaaaccata acccagggcc
     6661 gtttacgcat tgacgccaat tttaaacgtt ttgtggatga agaagtttta ccgggaacag
     6721 ggctggacgc tgcggcgttc tggcgcaatt ttgatgaaat cgttcacgat ctggcaccag
     6781 aaaatcgtca gttgctggca gagcgcgatc gcattcaggc ggcgcttgat gagtggcatc
     6841 gcagcaatcc ggggccggta aaagataaag cggcctataa atctttcctg cgtgaactgg
     6901 gctacctggt gccgcaaccg gagcgcgtga cggtggaaac cacgggcatt gacagcgaaa
     6961 tcaccagtca ggcggggcca cagctggtgg ttccggcaat gaacgcccgc tacgcgctga
     7021 acgcggcgaa cgctcgctgg ggctcactgt acgatgcgtt atacggcagc gacatcatcc
     7081 cgcaggaagg ggcgatggtc agcggctacg atccgcaacg cggtgagcag gttatcgcct
     7141 gggttcggcg tttcctcgat gaatctctac cgctggaaaa cggcagctat caggatgtgg
     7201 tggcgtttaa ggtggtcgat aaacaattac gcatccagtt gaaaaatggt aaagaaacca
     7261 cgttacgtac cccggcgcag tttgtcggtt accgtggcga tgccgctgcg ccgacctgca
     7321 ttttgctgaa aaataacggc ctgcatattg aactgcaaat tgatgccaac gggcggattg
     7381 gcaaagacga tccggcgcac atcaacgatg ttatcgtcga agcggccatc agtaccattc
     7441 tcgactgcga agattcggtc gcggcggttg atgcggaaga taaaatcctg ctgtaccgca
     7501 atctgctggg cctgatgcag gggactctgc aagagaaaat ggataaaaac ggtcggcaaa
     7561 tcgtgcgtaa actgaatgat gatcgtcagt acaccgccgc cgatggctct gaaatttctc
     7621 tgcacggacg ctcgttgctg tttatccgca acgtgggtca tttgatgacc attcctgtga
     7681 tttgggacag cgaaggcaat gaaatcccgg aaggcattct tgatggcgtc atgactggcg
     7741 cgattgccct ctatgattta aaagtgcaga aaaactcgcg cactggcagc gtctatattg
     7801 tgaaaccgaa aatgcacggt ccgcaggaag tggcgttcgc caacaaactg tttacccgca
     7861 ttgagacaat gctcggtatg gcaccgaata ccctgaaaat gggcattatg gatgaagaac
     7921 gtcggacctc gctgaacttg cgtagctgta tcgctcaggc gcgtaaccgc gtggcgttca
     7981 tcaataccgg tttcctcgac cgtaccggcg atgaaatgca ttcggtgatg gaagctggcc
     8041 cgatgctgcg taaaaaccag atgaaatcga cgccttggat caaagcctac gaacgtaata
     8101 acgtgctttc cggcctgttc tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt
     8161 gggcaatgcc ggacctgatg gcagacatgt acagccagaa gggcgaccaa ctgcgtgccg
     8221 gggcaaacac agcctgggtt ccgtcgccaa ctgccgctac gctccatgcg atgcactacc
     8281 accaaaccaa cgtacagagc gtgcaagcca acattgccca gaccgagttc aatgctgaat
     8341 ttgaaccgct gctggacgat ctgctgacta ttccggtggc tgaaaacgct aactggtcgg
     8401 tggaagagat ccaacaagag ctggataaca acgtgcaggg gattctgggg tacgtggtgc
     8461 gctgggtgga gcaggggatt ggttgttcaa aagtgccgga tattcacaat gtggcgctga
     8521 tggaagaccg cgcaacgctg cgtatctcca gccagcatat cgccaactgg ttacgtcacg
     8581 gtattctgac caaagaacag gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc
     8641 agcaaaacgc tgacgatccg gcttatcgtc cgatggcggg gaatttcgct aactcgtgtg
     8701 cttttaaagc tgccagcgat ttaatcttcc tcggcgtgaa acagccaaac ggctataccg
     8761 aaccgttatt acacgcctgg cgtttacgcg aaaaagaaag tcattaatag cgtcaaaaaa
     8821 gccccggcaa gttttattgt cggggctatg atagtaactg cggtagtaca tttatcccgg
     8881 aaataaccgg agcgattatc agggtttaat aatattaaat acccgtagcg cattactagc
     8941 gggcctaaaa caacaatatg gacatcttaa cgatactctg cgcctgcgtg tgcggaacag
     9001 aaacccttac ccgatatact tgttatgcca gaggtagcaa ttgcgttagc ggcgtactgt
     9061 tgctgtaaag tataacgtct atattttaca gttcagtcct cttttgctta ttacaacctt
     9121 aagtatatca agcatataaa gataataaga gactgaacaa tatggttacc tggacccaaa
     9181 tgtatatgcc aatgggagga ctggggctat ccgctctggt cgccctgatc ccgataatat
     9241 tcttcttcgt tgcactcgcg gtattacgtc tgaaaggaca tgtcgctgga gcaataaccc
     9301 ttatattatc tatcctgatt gcaatattcg cctttaaaat gccgattgat atggcttttg
     9361 ctgctgcggg ctatggcttt atttatggat tatggcctat tgcgtggatt attgtcgcgg
     9421 cggtgttcct ctataaatta accgttgcca gcggtcagtt cgatattatc cgcagctcgg
     9481 ttatctccat caccgacgat cagcgtttgc aggtgttact gattggtttc tcctttggtg
     9541 cgttgctgga aggagcggct ggctttggtg cgccggtggc gattaccggt gcgctgctgg
     9601 tgggcctggg cttcaaaccg ttatacgcgg cggggctgtg tctgattgcc aacactgcgc
     9661 cagtggcgtt tggtgcgttg ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg
     9721 atccgttcca cattggcgca atggcgggac gtcagttacc gttcctgtcg gttcttgtgc
     9781 cgttctggct ggtagcaatg atggacggct ggaaaggggt gaaagagacg tggccagcgg
     9841 cgctggttgc tgggggaagc ttcgctgtca ctcagttctt tacctctaac tatattggtc
     9901 cggaactgcc ggatattact tcggcgctgg tgagtatcgt ctcactcgct ttattcctta
     9961 aagtctggcg gccgaaaaat accgaaacgg caatcagcat ggggcaatcc gcaggtgcga
    10021 tggtggtaaa taagccatct tctggcggtc ccgtgccttc agaatatagt ctggggcaaa
    10081 tcattcgtgc gtggtcaccg tttttaatct taacggtgct ggtcaccatc tggaccatga
    10141 agccgtttaa agcgttattt gctccgggcg gcgcgtttta ttcactggtg attaatttcc
    10201 agatccctca tttgcatcaa caagtgttga aagcggcacc cattgtcgcc caaccaacgc
    10261 caatggatgc ggtgtttaaa ttcgaccctc tctcggctgg cggcaccgct atttttattg
    10321 cggcgattat ctctatcttc atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg
    10381 ccgaaacgct aattagtctg aagtggccga tattgtcgat tggcatggtg ctggcgttcg
    10441 ccttcgtcac caactattcc ggcatgtcca ccacgctggc gctggtactg gcaggtacag
    10501 gcgtgatgtt cccgtttttc tcaccgttcc tcggctggct gggggtattc cttaccggct
    10561 cggacacttc ttcaaacgcc ctgtttggtt cactgcaatc gactacggcg cagcaaatca
    10621 acgtctctga cactctgctg gtggcagcaa ataccagcgg cggcgtaact ggcaagatga
    10681 tctccccgca atctatcgcc gtggcctgcg ccgcgacggg catggtgggc cgagaatctg
    10741 aactattccg ctacaccgtg aagcacagtc tgatttttgc cagcgttatc ggcgttatca
    10801 cgttgttgca ggcgtatgtg tttactggaa tgttggtctc gtaaatcaat aatgccggat
    10861 gatcgttaat atcatccggc ttcatttaca ttattctgtc ttattaattg aaaattatt
//
LOCUS       DAZFYC010000153        10871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFYC010000153 DAZFYC010000000
VERSION     DAZFYC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.153"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            64..825
                     /gene="adiY"
                     /locus_tag="ACHKW5_004503"
     CDS             64..825
                     /gene="adiY"
                     /locus_tag="ACHKW5_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO7513741.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            962..2299
                     /gene="adiC"
                     /locus_tag="ACHKW5_004504"
     CDS             962..2299
                     /gene="adiC"
                     /locus_tag="ACHKW5_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO7513742.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2403..4046
                     /gene="eptA"
                     /locus_tag="ACHKW5_004505"
     CDS             2403..4046
                     /gene="eptA"
                     /locus_tag="ACHKW5_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO7513743.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4043..4711
                     /gene="basR"
                     /locus_tag="ACHKW5_004506"
     CDS             4043..4711
                     /gene="basR"
                     /locus_tag="ACHKW5_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO7513744.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4712..5812
                     /gene="pmrB"
                     /locus_tag="ACHKW5_004507"
     CDS             4712..5812
                     /gene="pmrB"
                     /locus_tag="ACHKW5_004507"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO7513745.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5788..5877)
                     /gene="pmrR"
                     /locus_tag="ACHKW5_004508"
     CDS             complement(5788..5877)
                     /gene="pmrR"
                     /locus_tag="ACHKW5_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO7513746.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5989..7491)
                     /gene="proP"
                     /locus_tag="ACHKW5_004509"
     CDS             complement(5989..7491)
                     /gene="proP"
                     /locus_tag="ACHKW5_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO7513747.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7755..8633)
                     /gene="rdcB"
                     /locus_tag="ACHKW5_004510"
     CDS             complement(7755..8633)
                     /gene="rdcB"
                     /locus_tag="ACHKW5_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO7513748.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRKHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8630..10765)
                     /gene="rdcA"
                     /locus_tag="ACHKW5_004511"
     CDS             complement(8630..10765)
                     /gene="rdcA"
                     /locus_tag="ACHKW5_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO7513749.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHAAYANASLYALRSAA
                     HKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQVEESLQLLQLNQAQVSGEIKHEIE
                     LALTSANHFLRQQQDALNAQLAALFQDDSEPLSEMRTRCETLLQTAQNTISRDFTLRF
                     AELESTLCRVLTDVIRPIEQQVKMELSESGFRPGFHFPVFHGVVPHFNTRQLFSAVIS
                     RQDATDEQSTRLGVVRETFSRWLNQPDWGRGNEKSPTETVDYSVLQRALSAEVDLYCQ
                     QMAKVLAEQVDESVTAGMNTFFAEFASCLTELQTRLRESLALRQQNESVVRLMQQQLQ
                     QTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2717 a         2829 c         2673 g         2652 t
ORIGIN      
        1 atttcccggt atcgctttgc tttagcgaga actggtcttt tattcgcact caggagtaca
       61 tgtatgagga tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg
      121 ataagggtga atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat
      181 tgtgaaaata atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt
      241 cacgacatca tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta
      301 tggcaacgta gcgctacgcc catactcacc ctgccatgcc tgacgccaga cgtctttcgc
      361 gttgccgcgc aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc
      421 gcattattat tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg
      481 atgtatatgt tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt
      541 cacaaagact ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta
      601 aagaaaaagt tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg
      661 cgttatgccg taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc
      721 tgcggctaca acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgacg
      781 ccgctgcatt atgttagtca gcacagagaa cgcactgtcg cctgattttt aaccttaacg
      841 aagagctata ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa
      901 tgcaggcgta tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat
      961 tatgtcttcg gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc
     1021 ggggaatatt atggggtcag gtgtttttct gttacctgca aacctggcct cgactggcgg
     1081 gattgccatt tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata
     1141 cgccaaaatg tcgttcctcg atccaagtcc tggtggttct tacgcttacg cccgccgctg
     1201 ctttggcccg tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg
     1261 caatatcgcc atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa
     1321 agatccattg gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa
     1381 cattgtcggt ccgaaaatga tcacccgtgt gcaggcagtt gccaccgtgc tggcgctgat
     1441 ccccatcgtc gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc
     1501 ggcatggaac gtcagcggcc tgggcacctt cggtgcaatt caaagtaccc ttaacgttac
     1561 gctgtggtcg ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc
     1621 gaaacgcaat gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt
     1681 actttctacc accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcttc
     1741 gccattcggt gatgccgcac ggatggcgtt gggtgacacc gccggggcca ttgtttcctt
     1801 ctgcgcagct gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac
     1861 ggcgaaagcc gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc
     1921 gggtacgcca gtggcggggc tgattatcgt cggtattttg atgaccatat tccagctcag
     1981 cagcatttca ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct cggtcatctt
     2041 tacactggtg ccatatcttt acacctgtgc ggcgttactg ctgctcggac acggtcactt
     2101 tggtaaagca cgcccggcat atctggcagt tactaccatt gccttcctct actgcatctg
     2161 ggccgtggtg gggtccggag cgaaagaggt tatgtggtca tttgtcaccc tgatggtcat
     2221 caccgccatg tatgcgctga attacaaccg gctacataaa aacccgtatc ccttagatgc
     2281 accaataagc aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac
     2341 ttaaggttgg cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg
     2401 tgatgttgaa gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg
     2461 ccgcttttta tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc
     2521 tgccgctgga ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtcgctttca
     2581 gcgtgattaa tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct
     2641 gcctgtttat tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca
     2701 tcgaccgctc gatgattgcc aatattattg ataccactcc ggcagaaagt tatgcgctga
     2761 tgacaccgca aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct
     2821 gctggataaa aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca
     2881 atattctggt ttctgtacta ctgattttgc tggtcgccgc actgttttat aaagactacg
     2941 cctcgttgtt ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg
     3001 ttgccagctg gtcatggtac tcccatcagc gactggcaaa tctaccgctg gtgcgaattg
     3061 gtgaagacgc ccaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc
     3121 tgattgtcgg cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa
     3181 ctaacccgcg gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca
     3241 cggcaacggc agtttcagta ccgtgcatgt tctcggatat gccgcgtgag cattacaaag
     3301 aagagctggc acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg
     3361 tgctgtggaa tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga
     3421 acgtcaccgc gctgaatctg cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc
     3481 tgttccacgg gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac
     3541 acaccatcgg cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat
     3601 ttaccccaac ctgcgacacc aatgagatcc agacctgtag caaagagcaa ctggtgaaca
     3661 cttacgacaa cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctga
     3721 aagaacatca ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt
     3781 taggtgaaaa tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa
     3841 aacaggtgcc gatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc
     3901 agaactgcct gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca
     3961 cgctattggg attaactggc gttgagacga agtattacca ggctgcggat gatattctgc
     4021 aaacttgcag gagagtgagt gaatgaaaat tctgattgtt gaagacgata cgctgttatt
     4081 gcagggattg attctggcgg cgcaaactga aggctacgcg tgcgatggcg tgacaaccgc
     4141 gcggatggcg gaacaaagcc ttgaggccgg tcattacagc ctggtggtac tggatttagg
     4201 gttacccgac gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct
     4261 gccagtactg atcctcaccg ctcgcgatac gctgaccgac aaaatcgctg ggctggatgt
     4321 cggtgccgat gactatctgg tgaagccttt tgcgctggaa gagttacacg cccgtatccg
     4381 cgccctgcta cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac
     4441 gctgaatatg ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa
     4501 agaatatgct ctgctgtcac ggttaatgct caaagcaggc agtccggtgc atcgggaaat
     4561 tctctacaac gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca
     4621 tatccacaat ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg
     4681 ctatatgctg gtcgcgaatg aggaaaacta attgaatctg atgcattttc tgcgccgacc
     4741 aatatcgctg cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct
     4801 gatcagcgtc ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc
     4861 gctacgcgac aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc
     4921 cagcctgatt gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc
     4981 ggtgcgccgc atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcactgc
     5041 cgacaactta acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc
     5101 ggcgttaaac gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttgtttac
     5161 cgctgacgtc gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact
     5221 gctggcgaaa acgcatcaca ttgatgtagc accgttagtg gcacgacttg atcagatgat
     5281 ggagagcgtc tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa
     5341 ttatcaacat gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac
     5401 catgctcgac cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt
     5461 tcagggtgat gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg
     5521 ttacagcccg caaggcagca acattatgat taagctgcaa gaagatgacg gagcggtcat
     5581 ggcagttgaa gatgaaggac caggtattga tgagagtaaa tgcggggagt tgagtaaagc
     5641 gtttgtacgt atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg
     5701 cattacacag ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac
     5761 gcgggcctgg gtacggctga agaaagatca gaacgtggca aaccagatat aaagaaagct
     5821 gctgaccacc agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc
     5881 actctcctcc gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag
     5941 ttcaattggc ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat
     6001 tcgcggatgt tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc
     6061 aatattatcg attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc
     6121 ctgtatatct gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat
     6181 ggttacgccg gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat
     6241 cagattctgc gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag
     6301 caccgaaata ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc
     6361 tggcaaggta gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa
     6421 cccggcaaaa atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa
     6481 cacaaacagg gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag
     6541 caagcccatc accggctgga caaacagcat accgatcata atggcgataa taatcagcac
     6601 cccgtggtct tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa
     6661 catgtagtaa gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca
     6721 gtgtttggtg gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc
     6781 gccctgttcc agtttatcga catgctgctg gaacgccgga gtttcttcca gcgcatggcg
     6841 caggtaaagc ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca
     6901 gtcgaggaag ttcgcttcgc cgacaatggt cgaaattaac accactacgc cagcacccag
     6961 cacaaacccg gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc
     7021 aggtgagtat tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc
     7081 ttgtgccatc ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga
     7141 cggtattaag ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat
     7201 cttctggcga ccatatttat cgcccaacat accaaagaag agcccgccaa gcggtcgaat
     7261 cagaaaggga acggagaacg tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc
     7321 cgggaaaaaa actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcaaacca
     7381 ttccattgca ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat
     7441 aatggtgaca tcacgaaggg taatcggttt tacttttttc cttttcagca tagctttcct
     7501 cgcagagttg gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg
     7561 agcgggaatt gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg
     7621 gttaaggaat gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta
     7681 ccctaccagt gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg
     7741 atgtaactga ttatttacgc caaatttaca gagaagattt cctcgaccat tgcccccgcc
     7801 agacgctgcg ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc
     7861 actgaccagc aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa
     7921 tcagcatgat ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga
     7981 cgcgtggtcg gggccgcatt gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa
     8041 tcacccagca acaacgccac cgcttgcggg cttcccagac gacaggcatc gccaaatacc
     8101 ccccaacgca actcttcgag aacgataaaa cagcgcccag gcaacgacca ggcgttatac
     8161 gccccagcac gccagcgggt aaaaatttgc tcgctctgtt cacccgcctg caccagtagg
     8221 cctcgttgcg ttaatgcctt ttccttccag cgacaacggg ctgaaaacca ctcatacaag
     8281 gcggcaattt gctgggggag ctccgggaaa gcagagtgct tgcggtgatc cattttttgc
     8341 agcagcgcca gatgctgcgc gatctgcgtc agcgccagat gaccgggatc cagcatcgac
     8401 gccagttttt ccaccattgt cagtcgcagg ctaagcggcg catttaacat gccgccgttc
     8461 gtccatgtct gaagctgcac acgcgaaaga agttcctgca tcagtcgttc acgaaactgc
     8521 tgctgatggg cttgtggaag gcgggcatca tcgccctgcg ccagatccac taaaaagcgg
     8581 ggataaaccg actccagcac gcgaccgggg ccgtccagta acgtcttggt caatatcgtt
     8641 ctgccgtgaa aagtgtttga atatcatcgc gtaacagctg ggcgtcggtg taaatccagc
     8701 cgtgagtcat cacagtctgc tgcaattgct gctgcatcag cctgaccacc gattcatttt
     8761 gttgacgcag agccaggctt tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga
     8821 actcagcgaa aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa
     8881 ctttagccat ttgttggcaa taaagatcga cttctgcgct taatgctcgt tgcaacacac
     8941 tgtaatcaac cgtttctgtc ggggatttct catttccccg tccccagtcg ggctgattca
     9001 accagcgcga aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg tccgttgcat
     9061 cctggcgcga aatgactgca ctgaacagct ggcgagtgtt gaagtgggga actacgccgt
     9121 gaaaaacagg aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt
     9181 gttgctcaat ggggcgaata acatcggtta acacccggca aagggtggat tccagctcgg
     9241 caaaacgcag cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac
     9301 agcgggtacg catctcgctt aacggctccg aatcatcctg aaacaaggcg gctaactgcg
     9361 cattcagcgc atcttgttgt tgacgcagaa agtggttggc ggaggtcagg gccagctcga
     9421 tttcatgttt aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt
     9481 cttcgacctg atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac
     9541 gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc
     9601 gcaaagcata caacgaggcg ttagcgtaag cggcatgacg ccagcgcctg ccgagcgcgg
     9661 catgggcaaa atcttccacc cagcgttgtt gctctggcgc tggtaactta ccgttgttgg
     9721 ctaactcatg gcgcgcccga ttcgccaggt agccccacat cgacgacacc ggaaatatct
     9781 gctgtggcgt aatacagcct ttcatcagcg tcccggaaat cagtgcccgc acctggtcgg
     9841 cgtcgtcact gttacgatcc tgttgatcga acttattgac cagcacatac agcggcaccg
     9901 attgccccac cgccaaaatc gcctcacgga cctcttcatc ggagatcgat ttcagttgcg
     9961 tataatccag caccgccagt accgccgagg cgcgtgccag ctgctggtta agcatttttt
    10021 gcagatgcgg ttgcccggct tcatttggcc cgggggtatc cagtaacgtc aactgaccgg
    10081 gataactctc cagccccgcc agatggacaa actccacttc aatcacggga atatgctcaa
    10141 tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc cttcgccagt cgcactaaat
    10201 cattcaaact tttcagacaa tgaaaaatag gctgggcacc cagataatat ttttcgaaag
    10261 cgacgccatt ttcgatccgc tgcataagcg cacgcatatc tttatctatt tccagcacat
    10321 cggtcagatg cttaatatcg caatcacgca ggcgctgttg taattgttga attaaacaat
    10381 cgattggcgc gacatgtgaa aaatgcagta ccggttcctt ttgcccgggc gtatggcgaa
    10441 taagcgtcgg cagcgcagtc attgggcgat tacgattagg cagaacctcc gtaccaacaa
    10501 tggcattaat ggtggttgat ttccctgctt tcatggtacc gacaattgcc agcaccattt
    10561 ccagtcggga aattttacgc aactcattat tcagcatcgc gtgacgttcg gcgatattag
    10621 gctgactcca gggtaaagcc agttgtggcg cgtcgtctcc gggtacagag agaggcattt
    10681 tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag gcgttcagcc tcctgactta
    10741 actcatacag ggtctgtgtg tacatagaaa attcttcctt aaagcaaatt ttgttacttt
    10801 tatttagcca gattgttttt gagttctgtt ttcggctttt ataattactg caagaaataa
    10861 ttttatattt a
//
LOCUS       DAZFYC010000154        10379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFYC010000154 DAZFYC010000000
VERSION     DAZFYC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.154"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHKW5_004512"
     CDS             complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHKW5_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO7513750.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHKW5_004513"
     CDS             complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHKW5_004513"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO7513751.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHKW5_004514"
     CDS             complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHKW5_004514"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO7513752.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4774..5511
                     /gene="bamD"
                     /locus_tag="ACHKW5_004515"
     CDS             4774..5511
                     /gene="bamD"
                     /locus_tag="ACHKW5_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO7513753.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5782..6123
                     /gene="raiA"
                     /locus_tag="ACHKW5_004516"
     CDS             5782..6123
                     /gene="raiA"
                     /locus_tag="ACHKW5_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO7513754.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6226..6351
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6227..6274
                     /gene="pheL"
                     /locus_tag="ACHKW5_004517"
     CDS             6227..6274
                     /gene="pheL"
                     /locus_tag="ACHKW5_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO7513755.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6373..7533
                     /gene="pheA"
                     /locus_tag="ACHKW5_004518"
     CDS             6373..7533
                     /gene="pheA"
                     /locus_tag="ACHKW5_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO7513756.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLKSAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHKW5_004519"
     CDS             complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHKW5_004519"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO7513757.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHKW5_004520"
     CDS             complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHKW5_004520"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO7513758.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9988..10353
                     /gene="yfiL"
                     /locus_tag="ACHKW5_004521"
     CDS             9988..10353
                     /gene="yfiL"
                     /locus_tag="ACHKW5_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO7513759.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
BASE COUNT         2668 a         2741 c         2430 g         2540 t
ORIGIN      
        1 tttttgcttt ctctgccgga attctcagga gaaccccgct gacccggcgg cgtgtttgcc
       61 gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac aagcgaaaaa
      121 tataaaaact ttatcgttcg ctcatttttc aggcaaaaca tcttaaatat agtcttttcc
      181 gtctaactta tagacaaaaa cgagccccga agggctcgtt ttatcattta ctggacggcg
      241 acaatccggt cttcattaac ttccaggcga atcactttac ccggaaccaa ttcaccagac
      301 agtatttgct gtgccagcgg gttttcgatc tgctgctgga tagcacgttt caaaggacgt
      361 gcaccataga ctggatcgta accgttctcg ctcagcagtt tcagcgcctc gtcagaaatg
      421 tggatttcat aaccacgttc ttccagacgt ttgtacagac gtttcaactg aatctgcgca
      481 atcgaggcaa tgtgctgttc acccagcgga tggaagacca ccacttcatc aatacggtta
      541 atgaattccg gacggaagtt atggcttacc acaccgagca ccagctcttt catgtgcgca
      601 taatccagtt caccgaagcg ttcctgaatc aaatcggaac cgaggttaga ggtcataatg
      661 acaaccgtat tacggaagtc gaccgttctc ccctgcccgt cagtcagacg accatcatcc
      721 agtacctgca acagaatgtt gaagacatcc ggatgcgctt tttccacttc atccagcagg
      781 atgacggaat acggacgacg acgcacagct tcggtcaggt agccaccttc ttcataaccg
      841 acatatcccg gaggcgcacc aaccagacga gacaccgagt gtttctccat aaactcggac
      901 atatcgatac ggaccatcgc ctcgtcgcta tcaaacataa agttcgccag cgccttacaa
      961 agctcagttt tccccacacc cgttgggccg aggaacagga atgaaccaat cgggcgattt
     1021 ggatccgcca accccgcacg gctacggcga atagcgttag ataccgcatc aaccgcttcg
     1081 ttctgaccaa tgacgcgatg gtgcagttct tgctccatac gcagcagttt ttcgcgctcg
     1141 ctttccatca tgcgagaaac cggaatcccc gtccaacgcg ccagcacttc agcaatttcg
     1201 gcgtcggtca ctttattacg caacagacgc atagttttgc cttcgagctg cgttgcggct
     1261 tccagttgct tttccagttc cgggattttg ccgtattgca gttcagacat ccgcgccagg
     1321 tcccccacac ggcgagcctg ttcaatagcg attttcgcct gttccagttc cgctttaatg
     1381 gtctgcgtac cagaaagcga tgccttctct gctttccact cttcttctaa ctcggagtac
     1441 tgacgttctt tgtcgctcag ttcttcgttg agcatatcca ggcgtttttt actggcttca
     1501 tcagactctt tcattaacgc ctgttgttcc agtttgagct ggatgatacg acgatcgagt
     1561 cggtcgagtt cttctggttt tgagtcaatc tgcatacgaa tgctggatgc tgcttcatcg
     1621 atcaggtcga tggctttatc cggcagctga cggtcagcaa tgtagcgatg agacaacgtc
     1681 gccgctgcaa caattgccgg gtcagtaatt tgcacatggt ggtgcaattc gtaacgttct
     1741 ttcaggccac gcagaatcgc aatggtatct tcaacagaag gctcggcaac aaacactttc
     1801 tggaaacgac gttccagcgc agcatctttt tcgatgtact ggcgatattc gtcaagcgtc
     1861 gtggcaccta cgcagtgcaa ttcaccacgc gccagcgccg gtttcagcat gtttccggcg
     1921 tccattgcgc catcggcttt acccgcgccg accatggtat gtaattcgtc gataaacagg
     1981 atgacgttgc cttcctgttt ggcaagatcg ttaagcacgc cttttaaacg ttcttcaaac
     2041 tcaccgcgat atttcgcccc agccaccagc gcgcccatat ccagcgccag tacgcggcgg
     2101 cctttcaatc cttccggcac ttcgccgttg ataatacgct gcgccagacc ttcaacgatg
     2161 gcagttttac cgacgccggg ttcaccaatc agtaccgggt tatttttagt acgacgttgc
     2221 agcacctgaa tggtacggcg aatttcttca tcacgaccaa tcaccggatc gagtttgccc
     2281 tgttcggctc gttcggtaag gtcgatggta tattttttca aagcctgacg ttggtcttca
     2341 gcaccttgat cgttcacgct ttcacctcca cgcatttgtt caatcgcttg agtaatgttg
     2401 gcggtggtcg cccctgctgc tttcaggatg tcggccagcg tgccgcgaga ctcaagtgcc
     2461 gccagaacga acagttctga cgagataaag ttatcaccac gtttttgcgc cagcttgtcg
     2521 caaagattaa gaacgcgcac cagatcctgt gatggctgga catcaccacc agtaccttca
     2581 acctgcggta aacgatttaa tgcctgattg atatctgtgc gcaactggcc agcatttatg
     2641 ccagcggatg ttaataaagg actaaccgaa cccccttcct gattcagcag ggcgctcatt
     2701 aaatgaagtg gttcgataaa ttggttgtcg tgcccgagtg caagtgattg ggcatcggca
     2761 agagcaagct ggaatttatt agtaagacga tccagacgca taactcctcc cataacggat
     2821 caaagttgct actggagatt aaatgaggtc atccctcaat tattcaaggt tattgaccag
     2881 attaatgtga aaagaaaatc acgcgtaccg gatcgtcttg attctttagg ttatatcagc
     2941 caaatgaaac ttgccatacg accggtggtc ttgtcgcgac gataagagaa gaaagtctca
     3001 ttttccgtat atgtacaacg gtcgccgccg aaaatttgct caacacccac gttcgccaga
     3061 cgctgccggg caagctgata aatatccgcc agatacttat caccatgctg aatgaaagct
     3121 gcacttgctt tagcgtctac tgccataaac gcctcgcgaa cctccacccc cacttcgaac
     3181 gcgcgtggac caattgccgg ccctaaccag gcgagaatat tttccggatt atcagcaaaa
     3241 caggaaaccg tctcttccag cacgcctgcg cacagtccac gccagccagc atgagcggcg
     3301 gcgacttccg ttcccgctcg attgcaaaac agcacaggga ggcagtcggc agtcatcact
     3361 gcgcaaaccg tgccgggcgt attgctataa gaggcatctg ctcgttttga ggcataaggt
     3421 tcgccagtaa gcttaagcac atctttgccg tgtacctgct caagccagac cggtttagaa
     3481 ggcaaattgc ccgcagcaaa aagtcgcttg cgattctcct caacgtgatc cgggttatcg
     3541 ccacaatggg caccgaggtt gagcgagtca tacgggggca aactcacgcc gccgatacga
     3601 gaagagctac aggccgcaac accttttggt agcggccact gcgggacaat cagcttactc
     3661 ataaccagtc cacttcatcc ttatgctctt cgaaatcggc gcgcatcacc tcaatcagct
     3721 ccaccatatc ttgtggaata ggcgcatgcc attccatttc gatgccggag atcgggtgat
     3781 aaagacgcag catggttgca tgaagcgcct ggcggtcaaa cttacgcagc atggagataa
     3841 atgcttccga agcacctttt ggcggacgcg gacggccacc ataaaccgga tcgcccacca
     3901 gcggatgagt gatatgggcc atatgcacgc ggatctggtg cgtacgtcca gtttccagac
     3961 gcaaccgcag acgcgtgtgc acacggaagt gttccatgat gcgatagtga gtcaccgccg
     4021 gtttgcccat cggatgcacc gccatgtgag tacgtttggt cgggtggcgg ctgatgggtt
     4081 catctactgt accgccagcg gtcatatgac caatcgccac cgcttcatac tcacgagtaa
     4141 tttcacgccg ttgcaaagat tcgactaaac gcgtctgagc cggaacggtt tttgccacaa
     4201 ccatcaggcc agtggtgtct ttatccagac gatggacgat gcccgcacgt ggtacatcgg
     4261 caatgggcgg atagtaatga agcaacgcat tcagtaccgt gccatccggg ttacccgcgc
     4321 caggatgtac caccaggtcg cgcggtttat taatgacgat gatatcttca tcttcataga
     4381 cgatatccag cgggatatcc tgcggttcaa aacgcgcttc ttcttcaatc tcagcgttga
     4441 tggcaacctg ctcgccaccc aatacttttt ctttcggctt atcacaaact ttgccgttta
     4501 ccagcacgcg ctggtcgagg atccattctt ttattcgcga acgtgaataa tccgggaaca
     4561 tttcggccaa agcctgatct aagcgttgac cgagttggtt ttcggacacc gttgcagtga
     4621 gctgtactcg ttgtgccata tatactgctt cttcgtttat cgttggggtt ttacggcttt
     4681 gccgtttaat atagtgtgct attgtagctg gtcttaaccg ggagcaggaa cagagaatct
     4741 cccgtattac attttgagga aagtcaaaac gtcatgacgc gcatgaaata tctggtggca
     4801 gccgccacac taagcctgtt tttggcgggt tgctcggggt caaaggaaga agtacctgat
     4861 aatccgccaa atgaaattta cgcgactgca caacaaaagc tgcaggacgg taactggaga
     4921 caggcaataa cgcaactgga agcgttagat aatcgctatc cgtttggtcc gtattcgcag
     4981 caggtgcagc tggatctcat ctacgcctac tataaaaacg ccgatttgcc gttagcgcag
     5041 gctgccatcg atcgttttat tcgccttaac ccgacccatc cgaatatcga ttatgtcatg
     5101 tacatgcgtg gcctgaccaa tatggcgctg gatgacagtg cgctgcaagg gttctttggc
     5161 gttgaccgta gcgatcgcga tcctcaacat gcacgagctg cgtttagtga cttttccaaa
     5221 ctggtgcgcg gctatccaaa cagtcagtac accaccgatg ccaccaaacg tctggtattc
     5281 ctgaaagatc gtctggcgaa atatgaatac tccgtggccg agtactatac agaacgtggc
     5341 gcatgggttg ccgtcgttaa ccgcgtagaa ggaatgttgc gcgactaccc ggatacccag
     5401 gctacgcgtg atgcgctgcc gctgatggaa aatgcatacc gtcagatgca gatgaatgcg
     5461 caagctgaaa aagtagcgaa aatcatcgcc gcaaacagca gcaatacata acagaaacct
     5521 gaaatacaaa acggcagccc ttgagctgcc gtttttttat tctgttagtt gtgaaactga
     5581 agcgatttag tcactatcga tctcatcaaa tatggctcgc tttgagatat tcctcaagta
     5641 aaaaaacacc tcttcctgcg atttctcaca aaaaagattc gttgacaaaa agtgacaaaa
     5701 ttatgagatt cccatcacac attttgacat caggagcggt atgctgaatt caccaagacg
     5761 ggaagacaag aggtaaaatt tatgacaatg aacattacca gcaaacaaat ggaaattact
     5821 ccggccatcc gccaacatgt cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat
     5881 ctgattaatc cacatatcat tctgtccaaa gagccacaag ggtttgttgc tgacgccaca
     5941 atcaatacac ctaacggcgt tctggttgcc agtggtaaac atgaagatat gtacactgca
     6001 attaacgaat tgatcaacaa gctggaacgg cagctcaata aactgcagca caaaggcgaa
     6061 gcacgtcgtg ccgcaacatc ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag
     6121 tagtccttta tattgagtgt atcgccaacg cgccttcggg cgcgtttttt gttgacagcg
     6181 tgaaaacagt acgggtactg tactaaagtc acttaaggaa acaaacatga aacacacacc
     6241 gtttttcttc gcattctttt ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac
     6301 agaatgcgaa gacgaacaat aaggcctccc aaatcggggg gcctttttta ttgataacaa
     6361 aaaggcaaca ctatgacatc ggaaaacccg ttactggcgc tgcgagagaa aatcagcgcg
     6421 ctggatgaaa aattattagc attactggca gagcggcgcg aattggccgt cgaggtagga
     6481 aaagccaaac tgctctcgca tcgcccggta cgagatattg atcgtgaacg cgatttactg
     6541 gaaagattaa ttacgctcgg taaagcgcac catctggacg cccattacat tactcgcctg
     6601 ttccagctca tcattgaaga ttccgtatta actcagcagg ctttgctcca gcaacatctc
     6661 aataaaatta atccgcactc agcacgcatc gcttttctcg gccccaaagg ttcttattcc
     6721 catcttgcgg cgcgccagta tgctgcccgt cactttgagc aattcattga aagtggctgc
     6781 gccaaatttg ccgatatttt taatcaggtg gaaaccggcc aggccgacta tgccgtcgta
     6841 ccgattgaaa ataccagctc cggtgccata aacgacgttt acgatctgct gcaacatacc
     6901 agcttgtcga ttgttggcga gatgacgtta actatcgacc attgtttgtt ggtctccggc
     6961 acgactgatt tatccaccat taatacggtc tacagccatc cgcagccatt ccagcaatgc
     7021 agcaaattcc ttaatcgtta tcctcactgg aagattgaat ataccgaaag tacgtctgcg
     7081 gcaatggaaa aggttgcaca ggcaaaatca ccgcatgttg ctgcattggg aagcgaagct
     7141 ggcggcactt tgtacggttt gcaggtactg gagcgtattg aagcgaatca gcaacaaaac
     7201 ttcacccgat ttgtggtgtt ggcacgtaaa gccattaacg tgtctgacca ggttccggcg
     7261 aaaacgacgt tgttaatggc gaccgggcaa caagccggtg cgctggttga agcgttgctg
     7321 gtgctgcgca accacaatct gattatgacc cgtctggaat cacgcccgat tcacggtaat
     7381 ccatgggaag agatgtttta tctggatatt caggccaatc ttaaatcagc ggaaatgcaa
     7441 aaagcattga aagagttagg ggaaattacc cgttcaatga aggtattggg ctgttaccca
     7501 agtgagaacg tagtgcctgt tgatccaacc tgatgaaaag gtgccggatg atgtgaatca
     7561 tccggcactg gattattact ggcggttgtc attcgcctga cgcaataaca cgcggctttc
     7621 actctgaaaa cgctgtgcgt aatcgccgaa ccagtgctcc accttgcgga aactgtcaat
     7681 aaacgcctgc ttatcgccct gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta
     7741 acgtttgatt aacgccagat tacgctctga cgacataata atgtcggcat aaagctgcgg
     7801 atcctgagca aacagtcgcc cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag
     7861 cgccagaagt tgctcaagct gaacattttc ttctgccaga tgcagcccat aagcaaaagt
     7921 agcaaagtgg cgcagagcct gaataaacgc catattctga tcgtgctcga cagcgctaat
     7981 acgatgcagc cgagcgcccc agacctgaat ttgctccaga aaccattggt atgcttccgg
     8041 cttacgtcca tcacaccaga ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa
     8101 catcgggtgt aaccccagta ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc
     8161 atttttcact gatgccagat caaccagaat acaatctttc ggtaaaggcg gtaatttgcc
     8221 gataacttgc tcagtaacgt ggattggcac actaacaatc accattccgg catcggcaac
     8281 aatatcagcc gctcgatccc agtcatgttg ctccagaatc cgcacctgat aacccgagag
     8341 ggtcagcatc ttctcgaaca ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg
     8401 acgcagtgac ggacaaagtg ttttaaatcc tttgtcgttt tcactggagt aagattcacg
     8461 catcacccga cgcaaaacat cctcaatcag atctggcggt acacccagag cttccgcctc
     8521 tgcacgacgc gaggccaaca tagatgcctc gcgctccgga acataaatag gcagtccaaa
     8581 gcggcttttc acctcgccca cttcagcaac cagttccaga cgcttagcta ataaattcag
     8641 cagcgcttta tcgacttcat caatttgatc gcgtaatgcg gtcaattcag caaccataat
     8701 aaacctctta agccacgcga gccgtcagca gcccgttcag atcctgatga atttcacgca
     8761 gcaaggcatc ggtcatttcc cagctaatgc aggcatcggt tacggataca ccgtatttca
     8821 tttcactgcg cggttgctcg gaagactgat tgccctcgtg gatattactt tcgatcatca
     8881 gaccaataat tgagcgattg ccatctttga tttgagcaac cacggattct gccaccgcag
     8941 gctgacggcg ataatcttta ttggaattac cgtggctgca atctaccatc agagacgggc
     9001 gcagtcccgc ctgttccatc tctttttcac attgcgcaac atccgcaggg ctatagttcg
     9061 gcgctttacc accgcgcagg atgacatgcc cgtccggatt cccctgagtt tgtagcaacg
     9121 caacctgccc tgcctggtta atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag
     9181 cgttaattgc tgttgccaga ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa
     9241 gcccggaggc catttcacgg tgagtttgcg attccgttgt acgagcacca attgctgacc
     9301 agctaaacag atcgcccagg tattgcgggc tattcggatc taacgcttcc gtcgccagtg
     9361 gcagtcccat attcaccagc tcaagcagca atttacgcgc gatctgcagc ccggcttcta
     9421 catcaaaaga gccatccata tggggatcgt taattaaccc tttccagccg acagtggtac
     9481 ggggtttttc aaaatagacg cgcattacca gatagaggct atcgctgacc tctgcggcaa
     9541 gggctttaaa tcgacgagca tattccagag cagtttccgg atcatgaatg gaacaaggac
     9601 cacatactac cagcagacga ggatcgcgcc cggcgataat atctgaaatg gttttacgcg
     9661 agtcagcaat ctgggcttct tgttgcaggc tcaatggaaa agcggccttc agttgttccg
     9721 gagtcattaa aacctgttcg tcggtaatat gtacgttatt cagcgcgtct ttttgcatga
     9781 tggcgatcct gtttatgctc gtttgcgata gttgatcctc agcgaggatg acgtaacgat
     9841 aacacataaa gtaaagtttt caatccatat ttcgtacatt tttatttaca caggcaattt
     9901 agtcgcgatt tcaaccctta cttctgtata gataaattta cacttccttt gaaaacaatc
     9961 cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct
    10021 ttactggtta gcggatgtca gattgatcct tatactcacg cgccaacctt gaccagcacc
    10081 gactggtatg atgttggtat ggaagatgcg atatcgggca gcgccataaa agatgacgat
    10141 gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc cgaaggacaa
    10201 aagaaaactt gccagaccga ttttacttat gcccggggac tttccggtaa aagctttcct
    10261 gccagctgca acaatgttga aaatgccagc caactgcatg aagtatggca aaaaggggct
    10321 gatgaaaatg ccagcacaat acgattaaat taattaaaac cccacaaata aattaaaca
//
LOCUS       DAZFYC010000155        10333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFYC010000155 DAZFYC010000000
VERSION     DAZFYC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.155"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            217..1899
                     /gene="ydeN"
                     /locus_tag="ACHKW5_004522"
     CDS             217..1899
                     /gene="ydeN"
                     /locus_tag="ACHKW5_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7513760.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1951..3108
                     /gene="ydeM"
                     /locus_tag="ACHKW5_004523"
     CDS             1951..3108
                     /gene="ydeM"
                     /locus_tag="ACHKW5_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7513761.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3074..3196)
                     /locus_tag="ACHKW5_004524"
     CDS             complement(3074..3196)
                     /locus_tag="ACHKW5_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513762.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3399..5084
                     /gene="yddA"
                     /locus_tag="ACHKW5_004525"
     CDS             3399..5084
                     /gene="yddA"
                     /locus_tag="ACHKW5_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO7513763.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            5122..7494
                     /gene="yddB"
                     /locus_tag="ACHKW5_004526"
     CDS             5122..7494
                     /gene="yddB"
                     /locus_tag="ACHKW5_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO7513764.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7551..>10333
                     /gene="pqqL"
                     /locus_tag="ACHKW5_004527"
     CDS             7551..>10333
                     /gene="pqqL"
                     /locus_tag="ACHKW5_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7513765.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMFETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRTT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     YPVNTYTGVLLPK"
BASE COUNT         3037 a         2276 c         2261 g         2759 t
ORIGIN      
        1 taaaatgcaa taatttatta aatatcaata gattagttgt ttatcggcaa gaaattactt
       61 agtagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt tttagctaaa
      121 tctgctatct ctttatgtga ctaacttcac ttacacccac ttatttctct tcgtaaaatt
      181 acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt aaagaaaagt
      241 gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt tgctgctcat
      301 gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc agactttacg
      361 ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat ggatgatctt
      421 ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat ggaaaatcgt
      481 gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc acaaaaatca
      541 acgccgacgc tcctttcatt aatggatgaa ggcgtacggt ttactaacgg ctatgtggca
      601 cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt
      661 ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac tttcttgcct
      721 gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca cttgtcaaaa
      781 atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga caacttcacc
      841 acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt tatgggattc
      901 cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc
      961 cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt tgttgatcgt
     1021 gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc gcacctgcca
     1081 aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag tcaaacagca
     1141 gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat tctcgaacaa
     1201 ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga taatggtgcg
     1261 gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag tcagacctat
     1321 cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca acccggtaat
     1381 tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc agccgatatc
     1441 agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt gcaagataag
     1501 aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca ctggtttgac
     1561 gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca gtcagacgat
     1621 tacccgcata accccaacac tgaggactta agccaattct cttatacggt gagaaataac
     1681 gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa actgacggat
     1741 ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga gatgcaaggc
     1801 gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa tcaggagaag
     1861 tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca tgcggcggaa
     1921 ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc ctccagtttt
     1981 caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca gtttactcat
     2041 gaaaaatgga tggatgacag tactctgaaa gagttcatca aacaatatat cgcagcgtct
     2101 ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt
     2161 ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat ttttaatgca
     2221 ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa agaacatgaa
     2281 tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta cagacgcagt
     2341 aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat
     2401 caagttgagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta ccctcttgag
     2461 gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctcgaa
     2521 accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat
     2581 ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt
     2641 aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag ccgttttttg
     2701 gggaatgggc atacaagttg tattttccag gagtcctgca aggataatct ggttgttgaa
     2761 agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac
     2821 attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg
     2881 atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg ttgtcctaag
     2941 catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg ttataaaatc
     3001 cttttttcaa ccatggtacc ttatatgaac gccatggtgg agttagctaa gaacagagta
     3061 ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata acgctctctt
     3121 ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca taaattcctg
     3181 tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt ggtatgcgtc
     3241 agggcattag caataccctt gattatcaat agcataacca taaccactga aaatgaaata
     3301 ttcctttata tttcatattg ttaaatgata cttaaataaa tttcatcagt aaatcattgc
     3361 catagataat cattggatgg cacaatcaat tttcgctaat gataaccatt cccattacgt
     3421 tttgtatgtt gatagcaaag tatctctgtc tgctgaagcc cttttggctg cgtaagaata
     3481 ataaaacttc agtgttgtta atcattatta tcctcgccat gatccttggc gtggttaaaa
     3541 ttcaggtctg gttaaacgac tggaataatg attttttcaa tgcgctaagc cagaaagaaa
     3601 ccgataagct ttggcagctt gtcctttggt tccctgccct gctggggatc tttgtattaa
     3661 tatccgtaaa caaaacatgg ttaattaaac tactgactat tcgctggcgc gaatggttaa
     3721 cagattacta ccttaatcgg tggtttgcag ataaaaacta ctacttcacg cagatctacg
     3781 gcgagcataa aaatacagat aaccctgacc aacgtatcgc tgaagacatt ctcttactga
     3841 tcagcaaaac gctcagcctt tcatttggct tcatccagtc acttagcatg ctgatcacct
     3901 ttaccgttat tctttggcaa agcgcgggta cgctctcatt cactgttggt ggaacagaat
     3961 ggaatatcca ggggtatatg gtctataccg ttgtgctcat tgtgatcggt ggaactttat
     4021 ttacccataa ggttggtaaa cgtattcgtc cgcttaatgt ggaaaaacaa cgtagcgaag
     4081 cgacatttcg aacaaatctt gtgcagcata ataagcaggc agaactgatt gcgctaagca
     4141 atgctgagtc tctacaacgc caggagttga gcgacaattt tcataccatt aaagagaact
     4201 ggcatcgttt aatgaatcgt cagcggtggc ttgattactg gcagaatatt tattcgcgct
     4261 cgcttagcgt tcttccctac tttctgttat tgccgcagtt tattagcggg cagatcaatc
     4321 tgggcggact gatgaaatcg cgccaggcat ttatgctggt atcgaacaat ttaagctggt
     4381 ttatttataa atatgacgaa cttgctgagc tggctgcggt tatcgatcgc ttgtatgagt
     4441 tccatcaact cactgaacag cgccctacga ataagcctaa aaattgccaa catgcggtac
     4501 aagtggctaa tgcgagtatt cgtacgcctg ataataagat catattagag aacttgaact
     4561 ttcatgtttc gccaggcaaa tggctattac tgaaaggcta ctctggtgcg ggaaaaacca
     4621 cactgcttaa aacattatcc cactgctggc cgtggtttaa aggtgatatt tcttctcctg
     4681 ctgacagttg gtatgtgtca caaacaccgt taatcaaaag cggcttactg aaagagatta
     4741 tttgtaaagc acttcccctg cccgtagacg ataaatcgtt gagcgaagta ctgcatcagg
     4801 ttggtctggg gaaattggct gcgcgtattc atgaccacga tcgctgggga gatattcttt
     4861 ccagcggcga aaaacaacgt atcgccctgg cacgattaat tttacgacgt ccgaaatgga
     4921 tatttcttga cgaaactacc tctcatcttg aggaacaaga ggctatccgc ttactgcgtt
     4981 tagtgcgtga aaaactaccc acaagcggcg tcattatggt tacacatcaa cccggtgtct
     5041 ggaacctggc agatgatatt tgtgacatta gcgcggtttt ataaaaacaa taaaagccct
     5101 gctgtacgga gatataaata aatgaagcga gttcttattc ctggcgtcat tttatgtggc
     5161 gctgatgtgg cgcaggccgt cgatgacaaa aacatgtaca tgcatgtttt tgaagagatg
     5221 acggtctatg ctcctgtccc tgtacccgta aacggcaaca cgcattacac cagtgaaagc
     5281 atcgagcgtt taccgaccgg gaatggcaat atcagcgatc tgctgagaac caaccctgcg
     5341 gtacgcatgg attcaacgca aagtacctcg ttgaaccagg gagatattcg cccggagaaa
     5401 atctctattc acggtgcgtc gccctatcag aatgcctatt tgattgacgg tattagtgcc
     5461 actaataact tgaacccagc gaatgagtcc gatgccagta gtgcaaccaa tattagcggg
     5521 atgtcacagg ggtattatct tgatgtcagc ttactggaca atgtgacgct ttatgacagt
     5581 tttgtgccgg ttgaattcgg tcgcttcaat ggcggggtaa ttgatgcaaa gatcaaacgc
     5641 ttcaacgctg atgatagcag cgtgaaactg ggttatcgca ctacgcgttc ggactggtta
     5701 acatcgcata tcgatgagaa taacaagagc gcatttaatc aaggctcttc aggaagtact
     5761 tattactccc cagattttaa aaagaacttt tataccttgt cgtttaatca ggaactcgct
     5821 gataactttg gcgttaccgc cggtttatcg cgccgccagt ctgatatcac ccgcgcggat
     5881 tatgtttcga atgacggcat tgtcgccggt cgggcacagt ataaaaacgt tatcgatacc
     5941 gcattgagca aatttacctg gtttgccagc gaccgcttta cccacgattt aaccttaaaa
     6001 tataccggct ccagccgtga ttataatacc agcaccttcc cgcagtctga tcgcgaaatg
     6061 ggtaataaat cctatggtct ggcatgggat atggatacgc agctcgcatg ggccaaacta
     6121 cgtaccaccg ttggttggga tcatattagt gattataccc gtcacgatca tgacatctgg
     6181 tacaccgaac tttcatgtac atatggtgat attacagggc gttgcacccg tggcggatta
     6241 ggacacattt cccaggctgt agataattac accttcaaaa cacgcctgga ctggcaaaaa
     6301 ttcgccgtgg gtaatgtttc gcatcaaccc tacttcggcg cggaatacat ctattccgat
     6361 gcgtggactg aacgccataa ccagtctgaa tcctatgtga ttaatgctgc cggaaagaaa
     6421 actaaccata ccatttacca taaaggtaaa ggcagcctgg gaattgacaa ctacacgctg
     6481 tatatggcgg atcgcattag ctggcgtaat gtgtcattaa tgcccggcgt gcggtatgac
     6541 tatgacaact atctgtcaaa ccacaatatc tccccgcgct ttatgacgga atgggatatt
     6601 tttgctaatc aaacctcaat gattacagca ggttataacc gttactatgg cgggaatatt
     6661 cttgatatgg gattacgtga tatccgcaat agctggacgg aatcggtatc aggtaataaa
     6721 actctgacgc gttatcagga tttgaaaacg ccttataacg atgaactggc aatgggattg
     6781 cagcagaaaa tcggtaagaa cgttattgcg cgtgcaaact atgtttaccg tgaagcgcat
     6841 gatcaaataa gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat
     6901 aacaacgatg gcaaaaccaa aacgcattcg ttcagcctca gttttgaact ggccgaaccc
     6961 ctgcatatcc gccaggtaga tattaaccca caaattgtct ttagctatat caagagcaag
     7021 ggcaacttgt cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt
     7081 tataacggta atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta
     7141 aagatctcct taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg
     7201 ctggcctggc aagaagcgcg taaagctcgc attatcctgg gtaagacaaa tgcgcaatac
     7261 atcagcgaat attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc
     7321 tgggacaccc gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt
     7381 gccgatattc tcaatgtatt ggatagcaaa accgctgttg atacaacgaa taccggtgtg
     7441 gcgacctacg ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc
     7501 ggattttact taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc
     7561 tctgtttctt actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg
     7621 ccttaccgca ggatgaaaag ttaattaccg ggcaactggg caatggcttg cgatatatga
     7681 tttatccgca tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt
     7741 cattgcagga agaagacaat gagcgcggcg tagctcattt tgtagaacat atgatgttta
     7801 acggcacaaa aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc
     7861 gttttggtcg cgatgttaat gcctatacca gctatgacga aacggtgtat caggtgagtt
     7921 tgccgactac gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta
     7981 atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat
     8041 ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg
     8101 ctaatacccg taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca
     8161 caccggcaca attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta
     8221 tcgtggtcgg cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta
     8281 agcttccggc taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc
     8341 tgcgctttaa tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc
     8401 gcctgccaat ggtacaagtg aacgatgagc aaagctttat cgaacaagct gaatggagca
     8461 tgttagttca gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga
     8521 ctatttctgg cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt
     8581 tccgtgtaaa tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt
     8641 tggcaaccat tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc
     8701 gcctcacctg gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga
     8761 ccagtcgcct ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat
     8821 atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc
     8881 agcagttaag aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg
     8941 ccaaaaaagc attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa
     9001 agctggcggc ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac
     9061 aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca
     9121 atggtgccag ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattactg
     9181 ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg
     9241 caaataaagc agtaagcgga agcggcgttg gtgagctctc ctcttccagc ctgaaacgct
     9301 ggagtgcgga aaattcggta accatgagca gtaaagtcag tggcatgaat acgttgctct
     9361 ccgttagcgc gcggactaat aaccctgaac ctggttttca gttgattaac cagcgaatca
     9421 cccacagcac gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt
     9481 tgaaaacgct cgaccagcgt ccagcggaga aattcgccca gcagatgttt gagacgcgct
     9541 atgctgatga ccgcacgaaa ttactgcaag aaaatcagat tgtacagttt actgccgcag
     9601 atgcgctggc tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg
     9661 tcggtaatgt ctcagaagac aaactcgtgg cgttaattac gcgttactta ggatcaatca
     9721 aacactctga ttcgccatta gccgcaggta aaccattaac tcgcacgacg gacaacgcat
     9781 cggttactgt aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg
     9841 attcccggac acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac
     9901 tggcaaaaga tctacgtgtt aatattcgtg aacaggcatc tggagcatac agcgtttctt
     9961 ctcgcctctc ggttgatcct caggccaaag atatcagtca tttgctggct tttacttgtc
    10021 aaccagaacg acatgatgaa ctgttaacgt tagcgaatga agtgatggtt aagcgtctgg
    10081 ctaaagggat cagtgagcaa gaactgaatg aataccagca aaacgttcag cgcagcctcg
    10141 atatccaaca gcgtagcgtt caacaattag cgaacactat tgtaaatagt cttattcaat
    10201 atgacgatcc tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga
    10261 atgttaacac tgccgttaaa caatatcttt cttatccggt aaatacttat accggagtat
    10321 tattgccaaa ata
//
LOCUS       DAZFYC010000156        10276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFYC010000156 DAZFYC010000000
VERSION     DAZFYC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.156"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(57..398)
                     /locus_tag="ACHKW5_004528"
     CDS             complement(57..398)
                     /locus_tag="ACHKW5_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7513766.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(391..3633)
                     /locus_tag="ACHKW5_004529"
     CDS             complement(391..3633)
                     /locus_tag="ACHKW5_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7513767.1"
                     /translation="MATLRELIIKISANSQSFQSELQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASG
                     VTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3684..3893)
                     /locus_tag="ACHKW5_004530"
     CDS             complement(3684..3893)
                     /locus_tag="ACHKW5_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO7513768.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(3989..4363)
                     /locus_tag="ACHKW5_004531"
     CDS             complement(3989..4363)
                     /locus_tag="ACHKW5_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO7513769.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLFDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4378..5094)
                     /locus_tag="ACHKW5_004532"
     CDS             complement(4378..5094)
                     /locus_tag="ACHKW5_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO7513770.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5161..5505)
                     /locus_tag="ACHKW5_004533"
     CDS             complement(5161..5505)
                     /locus_tag="ACHKW5_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO7513771.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5502..5948)
                     /locus_tag="ACHKW5_004534"
     CDS             complement(5502..5948)
                     /locus_tag="ACHKW5_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO7513772.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(5945..6295)
                     /locus_tag="ACHKW5_004535"
     CDS             complement(5945..6295)
                     /locus_tag="ACHKW5_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7513773.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6306..6632)
                     /locus_tag="ACHKW5_004536"
     CDS             complement(6306..6632)
                     /locus_tag="ACHKW5_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7513774.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(6629..9051)
                     /locus_tag="ACHKW5_004537"
                     /pseudo
     CDS             complement(6629..9051)
                     /locus_tag="ACHKW5_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(8997..9218)
                     /locus_tag="ACHKW5_004538"
     CDS             complement(8997..9218)
                     /locus_tag="ACHKW5_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513775.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(9263..>10276)
                     /locus_tag="ACHKW5_004539"
     CDS             complement(9263..>10276)
                     /locus_tag="ACHKW5_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7513776.1"
                     /translation="RSEALEVARRQYPDDSRLHHVLKSAVGAGTTTDPQWAGSLSEYQ
                     EYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTK
                     FDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAA
                     VADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMR
                     KNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREA
                     SLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSA
                     SGG"
BASE COUNT         2103 a         3412 c         2470 g         2291 t
ORIGIN      
        1 cggtgactgc tctgatttaa ccgactcgtt caggctttcc ccgtgaatat gctgcatcag
       61 ttcaccacct gctcaatcgt acagctgaaa tcactgtacc ggacgttatc cgtgacgctc
      121 cactcccggc acaccaccct caccgtccgg ttatgtttcg gcggtcgcca caaaaaggca
      181 cggtaaccac catgccagga taaaaattca tccagccagc gccgggttga ctcatccgtc
      241 acccggaaca ccgcctgaaa cgtcttcagt tgaggattca gtcctgtggg gcgacgctgt
      301 tcataaccgt caccaaaccg taccctcgcc accgacggtt tctcactcac ctgcatccct
      361 tcacgcggga ccagatgcag cgtttttatc tcagccactc agcattcctc cgtcacgtcg
      421 catggacaac atcaccgcct gcacccgctg gtcaatcagc tgcacaagac tgcctgccgc
      481 ctccggccct atctggccat tagtcccgtc attctgaatg gcgatatggt agaccgggga
      541 atacaccagg cccgcactac cgttcatact gcccaccgct cgcacaccca gcgagccatc
      601 cgccgcccgg gtcaggggca taatggcttc aggaccggcc tcccccatca gtcccgcccc
      661 ttttgcaaag gcaaagtacg tgggcgtatc cacaatactg ttgctgtacg cactcaggtt
      721 tgccgaggta tacacgccgc cttttgcatt ggccaccgcc ccgcccagcc agtcaccaat
      781 gctgccgaga aatcctcccg caccggacat accgtttgcc gccgtcttaa ttccgttgac
      841 aatcgcggca ttcataagaa cttttgatat ttcctgcagc actgatgagg cccagctgcg
      901 ccattccact ttatttccgt tcagcatctc cgtgatgtta ttcaccatcc ctgagatacc
      961 ctccgtcgca agctgtgctg cctgtgaggc gtaatcggac gcattatcca cccagttact
     1021 gaatccctcc tgcagccctt tctgccagtc cgcacgctgc acatccgatt cggcataaaa
     1081 ggctgcctgg tccttaaggc gttcgctcag atactgcgcg ttctgtgccc gtgcctgtct
     1141 gtaaaaatcc tcactgatat ccccggtctg atactgagac tgaaggtccg catccttctg
     1201 gcggaagctg tcgcggatct gctgcaactc ccgcatgcgt tccctggctc gttctccctg
     1261 cccgtatccc agcagttcgg cttcatttga tgcacgcgca gccacattat cattcttcag
     1321 ggtctcttcc cgggatcgca actgttcccg gattttttgc tggtcaatca gggccgcgtt
     1381 acgcagcagt tcctgcttct gcatctccgt cagggttttc agttcgccct gcgcagtctg
     1441 gtacttcagc ttcgccagct ctgtattctg acccgccagt gccagttgct ctttctgctg
     1501 cttcagtagc cgggaaaaac tgtcttccgc tttttccgtc tctgattttc caccccggga
     1561 tttaggtttg ttcgcctcgt tattacgcca ggcttccagg gcattactga tataacgctg
     1621 tctcgcctcc tgatacgaat cccccacaaa accaaggtca tccgccgcat accccagccg
     1681 ggcacgctct ttttcctccc ccttcagtcg ggacagggcc agctcacgct ctgtttttgt
     1741 cagggcactc tgctgtttat catccagggt ggcctgtggc agccgtaacg gtacattcac
     1801 cagcccctga cgctgctgaa gcagttcatt ccccagcccc agcagacggt tgaattccgt
     1861 atgctgaccg ttcataacca gcatggactg gtacacctta ttctgctctg ccgcctgctg
     1921 acgaattaac gccacacgac ggtcttccag cccggcaagc acatcctgaa tggactgcgc
     1981 tttttcctgc atctgtgcca gacgggactg ctcaacggca agctgctctg ttgcctgagc
     2041 aagcccttcc gttacggtct tcaccgaggt cagatggttt atcatgaatc cgtcaccggt
     2101 cgtccagccc gggttcgcca gaacatactg atatcctgcg attttttcct gcagggattt
     2161 cacccgactg gcctgttcat caatcagccg gttctgctct gtcagcgccg cccgtgttcg
     2221 tccttcatta tctgaggctt caggcaaaga cattgacggc gttttatgcg cgatttcatc
     2281 tatcgtcagt gcatactggc gcgcagactc cctggcctgc tcctgattct ggtacagcgt
     2341 gtaccatgct gcagccccca gcatcaccag tccgggtacg ccaccaacca gccccagcgc
     2401 accgctcatc agacgtgagc ccaccgccgt tgtactgttc agcgcattct gggcggcgct
     2461 tctggcagca atatttctgt tcaggcgttc ctgtgtggcc gccagacggg cctctgcagc
     2521 aatctgcatc tccgtcccgc gggctgccgc cacggcctgc tgagcacggt acacggctgc
     2581 ccttgcccgc gctgtggcaa tctgcgttcc cctgaactgt gcttccgcca gtgcaacttc
     2641 attacgtgca gccgtcacaa gtcctgccgt ggcagacatc gctccggagg ccatattgcc
     2701 aaagtaccgg gcaaccccga cggcaaccag cgcacccaca gctgttgcca cattatcaat
     2761 ctgtccggca acaccgttca gcacgccgga gagcgttttt gtcaccccgc tggcctcatt
     2821 cactccgccc acccaggcca taaaggcgtt ttccaccttc gtgatactac cggaaaccgt
     2881 ttctggcatg gcggcatatt catcacgcaa tacccccagc tggctgatta acgcaggaac
     2941 gactttatcc gccgtcagtt gaccatcgtc cgccatcgcc ttcagatctt tacgggccac
     3001 gcccatgcct gcagccagtg cacgaatgat ccggtcacca ctttcattga ccgaattaaa
     3061 ttcctcaccg cgcaacacac cctgtgccag cgcctggctg aactgggtga tcaccgaacc
     3121 cgcctcagcc gtactggcac cggagatttt cagccccgtg gaaatggcct ccgtcacctt
     3181 cagcacatca tcagcactgt aaccatattc acgcatcgag gcagccgaac gggcaaacag
     3241 ggccgcatta tccgaaaaag cggtacctgt ccgctggctg atatccatca gcactttctg
     3301 tgatgacgaa aattcatcag atgactgcga cgcctgtttc agacgggcat tcacggaact
     3361 ccactcatca gccagtgaaa tcaggtgtcc ggtggcaaag gcaccggcaa atgcgccagc
     3421 cattccgaca gcagaaccgc gaatttccgt caactggctg ttcagttctg ccagggcacg
     3481 tcgctgctcc cgggctgccg cagcggcctg acgcccgcca ttctgcaggg tccggtaata
     3541 ttcactgccc atacgggaag cacgctggag ctccgactgg aatgactgtg aatttgccga
     3601 aattttgata atcagttcac gtaacgtcgc cattcacctt tctccgggca aaaaaacctg
     3661 ccacagcagg ttttcatcat tatttatgac attgctgcaa agctcagcgc gtcttccagc
     3721 gccgcaaacg gatctgactc cactttatcc tcatcctcgc cccagcagag catggcgtcc
     3781 tccagtgaaa cattcatccc ctgcgccccg aaaaccgctt tcacgatctg cgcattacgg
     3841 atatccccgc gctcatcgcc cagcggggac accctgtcga actccatcca catcatcgcc
     3901 tcgctcgcac tcagactgtg gcccagttcg gataaggtgc gccccagacg gagcgcaagt
     3961 cgcatcagaa agagaatttc cgggcgggct acttttttct ggccgactct gcatcagcga
     4021 tcagttccag tgcctgacgc agcaaccggg catgtaccgg accatagacg gccagcacct
     4081 gctcacggtc gtccggagtg aacacccgct gcagatccgt atcaaacagg acatcgcaga
     4141 acagcgtcac atccgcttcc aggttacggc gggttttcgc caccaccgac agggtatcgt
     4201 catcctctcc atcaccattg agcacttcct gccacagata ccaggcctct gccgaaggct
     4261 cccgcagcac cacgctgaca ttaccccatt ccggcacctt caccgtttta tgacgaaacc
     4321 ctgacagtct ggccagcgcc agcgttttca gatccttttt catgatgacc catcccctta
     4381 tccggcggct gcgctcactg tcacggtgca ttcaacagac gtcacactct gtgctttctc
     4441 tgccgaatcg gtcaccacgc aggtatattt ccccgcatca gcggactgcg cacctggctt
     4501 actgaaggtg tctgtcgtct gcccgtcaac cggctgacca tccttcttcc aggcgtattt
     4561 atacggcggc gttcccccgt tggcactgac tgacattgtc agcagcgcac ctgtattcac
     4621 ggtaagtgtt ttatccagat ttttcacaaa cgccagcggt accacaaagg acaccggttt
     4681 gcctttcata cgcagtgaaa acgttgctgc caccacgccg ttggtaccgg atgaccaggt
     4741 gtgctgacgc acttccgcca ggaacttaaa gcccttaccg gacggaaaca gcaccttaaa
     4801 cgcatacaac gcgtcattgt cataggcatc acgcagggcg ttctgggcct gattcagata
     4861 aaaattaccc gacatggaaa tctcagacga cgcccccaga ccgttgatgt tctcctgctc
     4921 ggtggagcag agcgtggtca catcaatatc ctgtttctga ccggcggtga actggacttc
     4981 cttgatggtg cagtccaggc gcagatattc cgccttatcc atagtttcag cagtcgccgg
     5041 ggcagatgaa atcatcacct gcgtcagctg tgagcgttca tacaaagcag acattctgcc
     5101 tcctgataat aaaaaacccg cacgcggcgg ggtatgggtt ttgtagaaaa aaagaaaaag
     5161 tcacaccgtg acctgaaact ccagggttgc acggtaacag cggttttccg gaatatagtc
     5221 ctgcatttca ctgacggatc ccggggccag cagcattatg gcttcacggg cgtcctgacg
     5281 tatctgacgc gcctgcgtca cagtcccggc ataaacgtct atctgcaccg acactgagga
     5341 ctccgcctgc ccgcccatca cgtccgcaga caccgatgaa atcaggctga aaaccaccca
     5401 cggaagcgcc accgacggcc tgccatccag cagggggacc acatacgggt acacctgccc
     5461 gccggcaaga tgcgccagat gaggatacaa atccgcctcc gtcatcgtct cagtacctca
     5521 tcaatggccc ggttcatccg tgcaatcgcc acttgcgctg cctgttcact gcgcacatca
     5581 aacgccggac gcacaaacgg gtgcggtggc atattcacgg tccccatttc cacaaaccgc
     5641 cagtagaaag cattgcgcgg gttatccgcc ttcatggtgt tatcgctgtt accggtgtcc
     5701 ggattaacac cccggatatg cacaccggat tccatcccgc catcgcggga gcgccgggaa
     5761 aggaccacca cattgcggcg cagttttccc ctgcgtaccg gtgcccgtga caccacttct
     5821 tctttcagca cattcgcacc cgcacgggtt gcctcacgca gcacccggtt attttctgca
     5881 ccactcagaa gctgcaaatc gcggctgatg tcctccagcc ccgaaaaatc cagcagggtt
     5941 tcgatcattt ttcacctccc agccgacaga gaatttccag acgtccgccg gtcgcatccg
     6001 gcacgggcag cccgacaacg ttcaggatcc ggtcacgcca gggaccactc agcacatgaa
     6061 gtcgtgacgc tgccgtgatt tcccgaccgg actgaccgcg cacccagatg cggatttccg
     6121 cctgcgccat ttccgcaccg gactgcatcc gctcccggct gctcctgcca cggatatccg
     6181 catgaatttt cccgcatgac acccattctt ccgtcatttc tccggcagca ttacgggtta
     6241 acaccgggtt cagaacactt atcatctgtg tcagacgacc tgcagatatt gccattcctc
     6301 cctcctcata acaccgtcgg acaacgcaaa tcgtaaatca gcacggacac agaaaacggc
     6361 agttccccct gcacgaggtc ttcccgctca gcaagatccg gattccggta cagcatcccg
     6421 gtcagtcgca tggcagcccc cttcatccgg gttaatgcct cgcccgggat cagctcaccg
     6481 tcctcacgaa tcactttatc ccggctgccc tgaatgtagg ccagcagcac ggcggtagcc
     6541 tgacgaacct tgtccatcag catgtcatca tccgcgtcat ggtcaacacg cagatgtgcc
     6601 ttgatctctt ccagtgtcag taatgccgtc attttccgcc tcctgcatcc cgtccacgtt
     6661 ttgcagccag ggtccagcct gatgaatgag cttctccggg tttatcaccg gtcatactgt
     6721 tgcagtgcca cagcgagccc ccccacgtca ccgtatcgcc ggggtggtag gtttcaccgg
     6781 ctctgaacac accgcggtag agcatcaccg gcagggaaaa tgttttttcc gtacactggc
     6841 cactgctctg ccggatcacc acagagaaca accgctcatc cgtcatgctg acgtcgatat
     6901 ccgccacccc gtcaaccagg cattcccatc cccgcatccc gtgcgttttt tcatacgccc
     6961 gccagagtcc acccaggtgt gtggcatacg tgccccgggg aaaggatttt tgatcgtcaa
     7021 tagcggggag cacttccagt gccgtggcat cacgcccgtc ctgcggagcc ggaagggcat
     7081 tcaccgcctc cagaaccgcc tgcttcagta cttccggatc gtaatcacga ccatcacgcg
     7141 gagcagggat atggcttacg gcctctttca ccatctgctc aagcatcgga cgcacatcat
     7201 cgggggtgat acttttgccg tccgccggta ccggaatatt cgcaaccgca tcattcaccg
     7261 cctgcttcag tacttccgga tcataatcac gaccgtcacg cggagcaggg atatggctta
     7321 cagcctcttt caccatctgc tcaagcatcg gacgcacatc atcgggggtg atacttttgc
     7381 cgtccgccgg taccggaata ttcgcaaccg catcattcac cgcctgctgc agtacatccg
     7441 gatcataatc acgaccatca cgcggtaccg gaatggtccc cacagcgtca tccaccatcg
     7501 cctgcagaac cggatgtacc tcatccaccg tcacatgctt ctgtaatacc gccgacaggg
     7561 aagccagttt ctcttcaaac gcttgtgcct gcgcggccat cttcccctca aatgtgcgct
     7621 gtaaatccgc cagcaccgtg gagaattctt cgcccagtgc acgaataatg gacagttccc
     7681 gttccgtcat tttctcagta tccccctgaa catcgctttc actgcatcat gctctgtttc
     7741 actgattgcc ttattaccgt cagatgcgcc gtcaggcagt tgtgctgaaa ctgttttccc
     7801 ggccgacgcg aacgggtcct cacgggcatc acgacgggac agcgcctcca gactgtagtt
     7861 ctgctgctga agatacagtg catcaccgcc ggcaaggggc ggcaggttct cacgtttacg
     7921 ggcctcattg ggcgtgagaa gcgtattttt caccgattca cccagtgttt tcatgcgccg
     7981 ttcgctgtcc attctcagca gcgtggtgac gtcaaactcc gtgctctcgt tttcccccgt
     8041 ttccagcgcc tcatccagta acagctcaat ggactcaatc agcgtctgca gacactggga
     8101 ataatactgc tgctccagcg cctccacgtt gtcactggaa ggcgggtggc caacgccaat
     8161 cttgtaggcc gggacacgga acaccgaaca gacaatttca gcggtcatct tcagttgttc
     8221 caccgtctgc gcatccacag gtgaaaacgt cgtggggttg tattttgccc cgttgctcag
     8281 aatggccgtt ttccccgcat tttcgcctgt atacccgctg tcccagttgc tcttcagttt
     8341 tttcgcattt tcttccgtaa tactgccggg gatctcaatc accccggacg gcctgccgcc
     8401 atttctgaaa aaagacgtcg aattttcctg aatatgatgc ccctgcgtgg ccgccagccc
     8461 ggcggcatac accggcggca atcctataag cggatgaaaa aaacagttaa accggtcgtg
     8521 gatcacttcc cgggcaggca ccgtcaccgc ctccgtgatc ccgcagttcc ggtccggcgt
     8581 gatgcggtag aacacctcgc cgtcatccgc caccagaggt tcaacccggc tccagtccag
     8641 aatacgcagt tctttgatct gcccccgggc attacggatt ttcagcacca ccgtattgcc
     8701 atgacgcagt ttggcgttca gccacagttc aaaaaactgg atgcggttct gctgggcgtt
     8761 gggacgacga cagaggcggg caatatcccc ccggcgcgtt tccctgcgta tcccatgcgc
     8821 atccgtctgc ataagacgca gccgcatttt ggcgatatcc tgggatatca gcgaaataca
     8881 tgcaaacacc gcatgaaagg agaggacggc ttcaggatcg gctttcacgc cctgctgcca
     8941 ggcgccggaa aagggctcag ccaccgcctg aaacaggctg gtccagcccg cctctcttac
     9001 gtcacgtcct gatttctggt tttttcgggt tcgccgtaaa aggttccaca ttcgccatgc
     9061 tccgcatcac gtttcttttt ctgacctgcc ggacgtcgca ccgtgatgta ctccgccttt
     9121 cccaggcgaa ccagcacctc cgcacacggc tgtgccacat cacggatatc cccggcccgg
     9181 gcatcatgcg tgccctgcag atatcggatc tttgccataa cctgttacgg gaggcgcacg
     9241 cctcccgtcc tccttatcag actcagccgc cggacgcact gccgtagttc actccggtga
     9301 tcaccgccac cgccgcagta cggcgacgac gccagttgat ccagcgctcc gcacggatgg
     9361 ccacgctgcc tgtctggaac atggaaacca gctccaccgg ggacggcgtg gtactgtcgc
     9421 cggtcggctc agactgcatt tccagtgatg cctcgcggga catatccact gccacgccgc
     9481 cgtcatccgc cagataaata tccggggcat tcaccagcac cagctggtca cccacgtact
     9541 gggagacaat caccggcagc ccctggaagg agccacccag cagggtcatg tccgggtatt
     9601 ccttctgacc cagcgcattt ttacgcatgg acagtgccag ggcattggtg ctggacatca
     9661 gccagaccgc accggtgggc tgcaggtttg ctgccacaaa ctgtccaaac gcagcctctg
     9721 catccgcatc cgggttaccg gttgatgccg tgcccttcac atcatgggtg atggacgccg
     9781 gggagacatc tgccactgcg gcttttttcg ggtccacaaa gtctgtatcc agacgcgcca
     9841 ccaccgcttc cgccagcgca ttacggacca gtgcatcagc agccggactg gaaaaacgga
     9901 tcaattcttc cgtcagtacc gcaatggccg acaccttcgc atgactgaag gtgatggatt
     9961 caaaatcaaa cttcgtcagg ggttttgcct taccctcacc cacccagccg gcagcaccgc
    10021 cggacacctg ggcgtgcaca cggatattga atggcacctg acgaagtgca gggatcccgc
    10081 cctgaccaaa tcgcccgata atggtctgcg gacgcaggta atcaataaag tcctgtgcgt
    10141 attcctgata ttcagacagg ctgcctgccc actgcggatc cgtggtggtc cccgcgccca
    10201 ctgccgattt caggacatga tgcagacgac tgtcatccgg atactgacga cgggccactt
    10261 ccagggcttc agatcg
//
LOCUS       DAZFYC010000157        10206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFYC010000157 DAZFYC010000000
VERSION     DAZFYC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.157"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            289..1662
                     /gene="aroP"
                     /locus_tag="ACHKW5_004540"
     CDS             289..1662
                     /gene="aroP"
                     /locus_tag="ACHKW5_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO7513777.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1705..2559)
                     /gene="ampE"
                     /locus_tag="ACHKW5_004541"
     CDS             complement(1705..2559)
                     /gene="ampE"
                     /locus_tag="ACHKW5_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO7513778.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2556..3107)
                     /gene="ampD"
                     /locus_tag="ACHKW5_004542"
     CDS             complement(2556..3107)
                     /gene="ampD"
                     /locus_tag="ACHKW5_004542"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO7513779.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3195..4088
                     /gene="nadC"
                     /locus_tag="ACHKW5_004543"
     CDS             3195..4088
                     /gene="nadC"
                     /locus_tag="ACHKW5_004543"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO7513780.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4291..4731
                     /gene="ppdD"
                     /locus_tag="ACHKW5_004544"
     CDS             4291..4731
                     /gene="ppdD"
                     /locus_tag="ACHKW5_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO7513781.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4741..6126
                     /gene="gspE"
                     /locus_tag="ACHKW5_004545"
     CDS             4741..6126
                     /gene="gspE"
                     /locus_tag="ACHKW5_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO7513782.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6116..7318
                     /gene="hofC"
                     /locus_tag="ACHKW5_004546"
     CDS             6116..7318
                     /gene="hofC"
                     /locus_tag="ACHKW5_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO7513783.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7353..8396)
                     /gene="guaC"
                     /locus_tag="ACHKW5_004547"
     CDS             complement(7353..8396)
                     /gene="guaC"
                     /locus_tag="ACHKW5_004547"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO7513784.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8552..8596)
                     /locus_tag="ACHKW5_004548"
     CDS             complement(8552..8596)
                     /locus_tag="ACHKW5_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513785.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8621..9241
                     /gene="coaE"
                     /locus_tag="ACHKW5_004549"
     CDS             8621..9241
                     /gene="coaE"
                     /locus_tag="ACHKW5_004549"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO7513786.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9241..9984
                     /gene="zapD"
                     /locus_tag="ACHKW5_004550"
     CDS             9241..9984
                     /gene="zapD"
                     /locus_tag="ACHKW5_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO7513787.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9994..10191
                     /gene="yacG"
                     /locus_tag="ACHKW5_004551"
     CDS             9994..10191
                     /gene="yacG"
                     /locus_tag="ACHKW5_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO7513788.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2517 a         2675 c         2605 g         2409 t
ORIGIN      
        1 accaattttg tgatttttaa ttcaattaaa acgaatttaa attcattcta catattgaga
       61 ggggttgagg ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca
      121 taaagcgggt gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgt
      181 aacaattcaa acttctttga tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac
      241 acacgcatca ctgcgtagat caaaaaaaca atcaccgcac gaggtttcat gatggaaggt
      301 caacagcacg gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg
      361 ctgggtggcg cgatagggac agggctattc ctgggtagcg cctccgtaat acagtccgca
      421 gggccaggga ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt
      481 cagctgggtg aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat
      541 aaatactggg gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt
      601 ttagttgcca tggctgagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa
      661 atccccacct gggtttctgc cgccgtattc tttgtggtga ttaacgccat caacctgacc
      721 aacgttaaag tgtttggcga gatggagttc tggtttgcca ttatcaaagt tatcgcggtg
      781 gtagcgatga tcatcttcgg cggctggctg ctattcagtg gcaacggcgg cccgcaggcg
      841 accgttagca acctgtggga tcagggtggt ttcctgccgc acggcttcac cgggctggtg
      901 atgatgatgg cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca
      961 gcagaagctg ataacccgga gcaaagtata ccgaaagcaa ctaaccaggt tatctaccgc
     1021 atcctgattt tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc
     1081 gttaccgccg ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg
     1141 gcgaatgcgc tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta
     1201 tattgcaaca gccgtatgct gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg
     1261 gcgtctgtcg ataaacgtgg tgtaccagta aataccattc tggtgtctgc actggtaacg
     1321 gcgttgtgcg tactgattaa ctaccttgcc ccagagtccg ctttcggact gttaatggcg
     1381 ctggtggtat ctgcactggt aatcaactgg gcgatgatta gcctggcgca tatgaaattc
     1441 cgtcgcgcca agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg
     1501 ggtaactgga tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca
     1561 ggaatggcga tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat
     1621 ctgtttaaag agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc
     1681 cgtacagggt gagggcaata atctttacac caacgccccg taaatcgtca gtagtgcaat
     1741 caccaccacg accacgaacg aggttttctt cgccattgaa accgctgcct tcggcgtctc
     1801 caccttatcg acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaacacctg
     1861 atactgcgaa gtatggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc
     1921 atgaccgatc aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg
     1981 aagcacggca tcaatgccgg actgtaaacg atgatgcggc gtctgatatc gcgccagcca
     2041 gtattgccat gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc
     2101 cacaatcagc cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt
     2161 ttgcagctca cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt
     2221 gagttcgcca gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata
     2281 agcatgataa tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca
     2341 caccagtagc gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt
     2401 cacgcccatc gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg
     2461 ccgaaagaac gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg
     2521 ctcgaaaatt aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc
     2581 acacgaaacc gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata
     2641 tcacaatggc ccgtcatgtt tttagcgata tccggatagc aatcaatcag tgcccgcgta
     2701 accgccgcaa gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc
     2761 tcaatcccaa tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca
     2821 tgccatgcac gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa
     2881 cagtgagcgg agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca
     2941 atagttccag tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta
     3001 atattgtgca ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt
     3061 gagggaacgc ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt
     3121 atatgtggtg ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta
     3181 tctggagttt taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc
     3241 tggaacgcat taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag
     3301 gcggaacagt cgatgccaac aatgatatta cggcaaaact tttaccggaa aattctcgct
     3361 ctcatgccac ggtgatcacc cgcgagaatg gcgtcttttg cggcaaacgc tgggttgaag
     3421 aggtgtttat tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg
     3481 atgtcatcaa tgccaatcaa tccttgttcg aacttgaagg cccatcccgc gtgctgttaa
     3541 cgggcgaacg cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac
     3601 gccactatgt cgaattgctg gaaggcacca acacgcagtt gttggatacg cgcaaaacct
     3661 tacccggcct gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc
     3721 gtctggggct ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag
     3781 tgcgccaggc ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag
     3841 tagagaatct ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg
     3901 ataacttcga aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac
     3961 tggaagtgtc tggcaacgtc actgacaaaa cactgcgtga atttgccgaa acgggcgtgg
     4021 actttatctc cgtcggtgcg ctaactaaac acgtacaagc actcgacctt tcaatgcgtt
     4081 ttcgctaata gttgacagat ttatccgctc catcgcgggc ggataatctc cccttcccca
     4141 actttcttcg taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc
     4201 ggaatctgct ttcccgtttc gttttttgcc cctcgcctgt gctgatggcg tttgccaaag
     4261 tagcgccaac caaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc
     4321 gaactgatgg tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat
     4381 caaaactacc tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt
     4441 accgccgtag agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc
     4501 aatggcattc cctcgcctac caccacccac tatgtttcag ccatgagtgt ggcaaaaggc
     4561 gtggtgtcgc tgaccgggca agaaagtctc aatggactaa gcgtcgtcat gacaccgggt
     4621 tgggataacg caaacggcgt caccggctgg acgcgcaact gcaatattca aagtgacagc
     4681 gcattgcatc aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca
     4741 atgaatattc cacagctcac tgccctgtgt ctgcgttatc atggagtctt gctggatgcc
     4801 agcgaagagg tggttcatgt tgcggtagtc gatgcacctt cgcatgagct actggacgca
     4861 ttgcatttcg ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa
     4921 ggtcacgcca gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc
     4981 aaagcagagt tgctaactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt
     5041 catatcgaac cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat
     5101 cctttaccgg atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg
     5161 ggaaacctgg atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg
     5221 gcaggaaacg ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg
     5281 gtattaaggt tgttacagca ggtgagccag gcactggatg tcaacacgct tggaatgcag
     5341 ccgttacaac tggcggactt tgctcatgcc ttgcaacaac cacagggact ggtgctggta
     5401 actggcccta caggcagcgg caaaacggtc acgctttata gtgccctgca aacgctgaat
     5461 accgctgaca ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta
     5521 aaccagacgc aaatccatcc gcgtgccgga ctcacctttc agggcgtgtt gcgtgcgtta
     5581 ttgcgccagg atcctgacgt catcatgatc ggagagatcc gcgatggcga aacagcagag
     5641 atcgctatta aagcggcgca aactggtcac ctggtgttgt ctaccctaca cactaattcc
     5701 acctgcgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg
     5761 gcgcttacgc tggtaatagc ccagcgtctg gtacgcaaac tttgcccaca ttgtcgccgg
     5821 cagcaagggg agcccatcca cattccagac aatgtatggc catcgccgct gccccactgg
     5881 caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc cttatttgaa
     5941 gttctgccca taacgccggt cattcgtcag cttatttccg ctaataccga cgttgaatcg
     6001 ctggaaacgc acgcacgaca ggcgggtatg cgtacgcttt ttgaaaacgg ctgcctggcc
     6061 gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc
     6121 gagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat
     6181 gctatgggca gagagccgtg ctttgctgct catggcacta cagcaacaga tggttacccc
     6241 acttagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga
     6301 agtcattcat caactggcga cgctactcaa agccgggtta acgctttctg aagggctggc
     6361 actgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca
     6421 cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag aggtatttcc
     6481 gccactctat caggcaatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg
     6541 ctttgaactg gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag tgaaatcagc
     6601 gttacgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca
     6661 ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact
     6721 aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggagctggc tgctggtgtt
     6781 gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgaccga cctggcttat
     6841 agtgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatgc ggggacaaaa
     6901 actcacgcag atttttacga ttctggcgct gacacaaagt gcaggcatta cttttttgca
     6961 gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca
     7021 aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa ataccgggga
     7081 gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct cgctggacct
     7141 catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg cggataacct
     7201 cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt
     7261 ggtggcgatg tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg
     7321 ctggcgtgtt gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct
     7381 tgttcctgca cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca
     7441 acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg
     7501 cctcgcagcg gcagcttaac ggttttacct tctgctgcgc gatattccgc aacgccgcca
     7561 acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg
     7621 ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg
     7681 acgaaatcgg caccgccgcc aaaggctttt gcgacatcgc ccggcgtggt gcagccaccg
     7741 tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat taccgcagaa
     7801 agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg
     7861 ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc accagtcact
     7921 acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc
     7981 acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa tgccgggttc
     8041 aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc
     8101 acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa
     8161 tagtgtttat gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat
     8221 gtacctacgg tgtccatatt tgcggcgata attggcacgc cggaccagct ctgacctgaa
     8281 tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt
     8341 ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc
     8401 ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg
     8461 cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa
     8521 tttacctggt aaatagtgag cgaacgtttc atcaatttcg ctttcgtgca ctcattttta
     8581 acgttttgat aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt
     8641 aacgggaggc attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat
     8701 taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct
     8761 acatgccatt gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg
     8821 ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct
     8881 gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta
     8941 tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt
     9001 gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt
     9061 aactcgcgag catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc
     9121 cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc
     9181 ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta
     9241 atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc
     9301 attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg
     9361 ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc
     9421 actgagctgt tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg
     9481 cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc
     9541 gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg
     9601 gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac
     9661 atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc
     9721 ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggctccc
     9781 ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg
     9841 ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc
     9901 cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc ggaacgtctg
     9961 gatttcgaac tggcctgttg ctaaggagtg aaaatgtcag aaactattac ggtgaattgc
    10021 ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc
    10081 aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc
    10141 agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg acatttgtct
    10201 gatgcc
//
LOCUS       DAZFYC010000158        10174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFYC010000158 DAZFYC010000000
VERSION     DAZFYC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.158"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="ACHKW5_004552"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="ACHKW5_004552"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            212..287
                     /locus_tag="ACHKW5_004553"
     tRNA            212..287
                     /locus_tag="ACHKW5_004553"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Thr,seq:ggt)
     gene            327..442
                     /gene="rrf"
                     /locus_tag="ACHKW5_004554"
     rRNA            327..442
                     /gene="rrf"
                     /locus_tag="ACHKW5_004554"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(674..1432)
                     /gene="yhdZ"
                     /locus_tag="ACHKW5_004555"
     CDS             complement(674..1432)
                     /gene="yhdZ"
                     /locus_tag="ACHKW5_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO7513789.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1440..2543)
                     /gene="yhdY"
                     /locus_tag="ACHKW5_004556"
     CDS             complement(1440..2543)
                     /gene="yhdY"
                     /locus_tag="ACHKW5_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7513790.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2553..3734)
                     /gene="yhdX"
                     /locus_tag="ACHKW5_004557"
     CDS             complement(2553..3734)
                     /gene="yhdX"
                     /locus_tag="ACHKW5_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7513791.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3802..4827)
                     /locus_tag="ACHKW5_004558"
     CDS             complement(3802..4827)
                     /locus_tag="ACHKW5_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7513792.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKLGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5258..5479)
                     /locus_tag="ACHKW5_004559"
     CDS             complement(5258..5479)
                     /locus_tag="ACHKW5_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513793.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5732..8836)
                     /gene="acrF"
                     /locus_tag="ACHKW5_004560"
     CDS             complement(5732..8836)
                     /gene="acrF"
                     /locus_tag="ACHKW5_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO7513794.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8848..10005)
                     /gene="acrE"
                     /locus_tag="ACHKW5_004561"
     CDS             complement(8848..10005)
                     /gene="acrE"
                     /locus_tag="ACHKW5_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO7513795.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2699 a         2625 c         2460 g         2390 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 gatctgataa aacagaattt gcctggcggc cttagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaatttagcg tgctgatatg gctcagttgg tagagcgcac
      241 ccttggtaag ggtgaggtcc ccagttcgac tctgggtatc agcaccactt tttaggttaa
      301 agttcggcag attagaaaag aatttgtctg gcggcagtag cgcggtggtc ccacctgacc
      361 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      421 cgagagtagg gaactgccag acatcaaata aaacaaaagg cccagtcttc cgactgagcc
      481 ttatgtttta tttgatgttt gtcggtgaac actctcccaa ccaggacaaa tccgccggga
      541 acggatttga acgttgcgaa gcaacggccc ggagggtggt gggcaggacg cccgccataa
      601 actgccagac atcaaatcaa gcaaaaaggc catccgaaag gatggccttt ttgcttttcg
      661 aactaacatt caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag
      721 gatgcgtaaa aaattcatca ggtgcagctt gctccactat ttccccacga tccataaaaa
      781 tcacccggtc agcgacggtt cgcgcaaacc ccatctcatg cgttacacac aacattgtca
      841 tacccgattg cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga
      901 gcgccgacgt tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa
      961 tggcaacgcg ttgctgctga ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg
     1021 caattctcac ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct
     1081 tgcgtaccca aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat
     1141 tgaaatgctg aaagaccatt cccacttcct gccggacgcg ctcaatattg cggatatctt
     1201 cattaagttc gatgccatct accacgatcc gtccctgttg atgttcttcc agatgattaa
     1261 tacaacgaat ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc
     1321 ccggttgcac ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga
     1381 cgttttccag cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct
     1441 caatgcggtg tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg
     1501 ctgaaacaga agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc
     1561 aaccaggtgg gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata
     1621 atgatcacca ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt
     1681 accagcttca acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc
     1741 agcgactctg ccgcttcata ttgcccttta ggcagcgcct gtaatccgcc tcgcacgact
     1801 tccgcaacat atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg
     1861 atattggtgc cttctgccat aaacaaaggc agcatgaccg aagacataaa caatacggta
     1921 atcagcggta cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc
     1981 atatgggagc ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca
     2041 acggatgcaa taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctcc
     2101 agaccaaaaa aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag
     2161 accgcaatat agcgaccgcg atgcgggagt attttccaga acattggtgc gattgacaca
     2221 aggccaatca gtaatgccag gttaattcgc cagcgttggt cgtgtgggta aagtccatac
     2281 ataaactgac caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg
     2341 gcacgcgttg agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc
     2401 agttcccaca tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac
     2461 agattttttc gcacccacac catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc
     2521 tgagacagca atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg
     2581 ttatagatat tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc
     2641 atggctatcg tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa
     2701 accatatcgg gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac
     2761 tggctggtta acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc
     2821 gtaaccgggt tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc
     2881 tggatcccag cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc
     2941 gttaaggctg ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg
     3001 gctgggacat cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca
     3061 atgatcaaca ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat
     3121 gttttattaa agcggaatag cccaacagaa aggactatag ccataacaac agccaggata
     3181 aacgcaataa atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa
     3241 aaggccagat caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca
     3301 aagtaccaga agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt
     3361 gtggatagtt ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagcct
     3421 aaaacagaag cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca
     3481 ataaaaacgc gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg
     3541 aagccagcgc cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc
     3601 gttacagtgt tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc
     3661 tggaataacc aggcgcgaac cgtagggttg gcaaaagaga gtgagccttt aacggttgag
     3721 cggcgatgag acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga
     3781 cgcccgttac ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt
     3841 ccagagatta ttttgcccaa gtttaatttt cagcgggctt tctgaaccta cgttacgctc
     3901 gaaaatttcc gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccag
     3961 cttcagatcc ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc
     4021 tggattagcc gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag
     4081 catggcgaaa agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac
     4141 cggcccaagc ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct
     4201 taatttgatg cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc
     4261 agattccagt gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat
     4321 attgtttgcc ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca
     4381 gacggtagcg ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa
     4441 gccaatgccg tcgtaataag tgacgccagt aaatgccatt cccatcccgg cgtcgcgaga
     4501 tgaagtccag gtcgtattac gggagagcaa atccacttcc cctgactgta aagccgtgaa
     4561 gcgttctttt gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc
     4621 ggcaacacca cgacaaacat caacatcaat acctgaaaac ttaccgtctg catcggcata
     4681 agagaaccca ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc
     4741 atcaagcgtc gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc
     4801 cagtgtggct atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata
     4861 ggacgtttgg gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg
     4921 ccagcttttg cgcccgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa
     4981 acagcgcccc gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta
     5041 cgcgtgtttt actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggaagtg
     5101 tgaaagttaa attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa
     5161 atcagcagag aattagccag cagaatatag caatatttta tcttatgtaa agatactgta
     5221 tcgttaaaca gagcgcgaaa ctccgcgccc tgttcagtta tttcgacaga gcgatgatcc
     5281 ccatcgtcag gccagcaaca gccgctgccc cgccggcaat agccccagcc gtttcccagt
     5341 tatcctgagt cgtgcctttc atacaggtat tggtgtgaac cagacgaccc tgatcgtcat
     5401 aaaccggaac gcagggcgaa tcgtgagcac agcccgccag caatgcggtg agcaatgcca
     5461 caggaattaa tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa
     5521 ctctcttatt aacgagctga atgccatttt atcaccggag gttataaata ttgcggaatg
     5581 aataatgaca aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaaagtt
     5641 tttaaagagt gagtggaaga atatcttatt aatttttatg ggtttcactg aaaataataa
     5701 aggcacccga aagtgccttt atgtttctga tttatccttt aaagcaacgg cggatcacca
     5761 caaagaacac cggtacgaag aagattgcca gcaacgttgc agagaccatt ccgcccatta
     5821 ccccgatacc cactgcgttc tgcgcgccac tgccggcacc gttactgata gctagcggta
     5881 atacgccgag aataaaggcg agagaggtca tcaggatagg acgcagacgc atacgtactg
     5941 ccattagtgt cgcttcaaca acacctttac cctctttctc catgagatct ttagcgaact
     6001 caacaatcaa aatagcgttt ttggccgaca agccaattgt cgttagcaag cccaccataa
     6061 agtagacgtc atttttttga ttaaagagtg tcgccgccag cagcacgccg acaatcccta
     6121 acggcacgac caacataacc gagacaggaa ttgaccagct ttcatagagt gcagcaaggc
     6181 acaggaaaac aaccacaaag gaaattgcta ccagagcggg agcctggttt cccgataagc
     6241 gttcctgata cgacataccc gtccagtcat aaccaatgcc agcaggtaat tttgacgcaa
     6301 ggttttccat caacgccatg gcatcgccgg aactggttcc tggcgcggct tccccctgaa
     6361 tctccattga cggcagaccg ttgtagcgtt ccagtcgcgg agagccatac acccaatgtg
     6421 aagtggtaaa ggccgagaat ggcaccattt cgccgttggc gctgcggaca taaagtttat
     6481 cgacatcttc tggcagcata cggaatttgg catccgcctg aacatacacc tttttcacgc
     6541 gaccacggtc gatgaagtcg ttaacgtaag tcccacccaa cgccgttgaa atggtctgat
     6601 tgatgtcaga aagtgaaaca cctaatgcct gcgccttttc ctggtcaact tccagtttaa
     6661 actgcgcggt gtcttccagg ccattaggac gcacgctgac taagctggca ggatgttgcg
     6721 ccgccatacc aagcaactgg ttacgggcct gggttagggc atcgtgaccc agcccagcct
     6781 gatcaattaa ctcaaagtcg aaacccgttg ccgtgcccag ttcaacaatg gctggcatat
     6841 tgaatggaat gacaaaaccg tcgcggatct tgccaaattc cattttggca cgatggatta
     6901 ccgcttccgc actgttttca tcaccattac gctcttccca cggtttcaga ctgacgaagg
     6961 ccataccggc attttgtgcc tggccgctga agctaaagcc gttaaccgta aagacacttt
     7021 caacgttcgc tttctcgttc ttcagatagt aatccgtcac ttgatccaac actttttgcg
     7081 tccgctcttg cgtcgcgcca gcgggtaact gaatcatggt cagaaagaca ccctgatcct
     7141 cttcaggtaa gaaggaagac ggaagacgta aaaacaacac caccattcct gcaacaatca
     7201 gcgcatagat cagtaaatat cgtcctgtgg aaccgaggat tttgccgacg ctgttggtgt
     7261 agtggttaac gctatgatcg aaggtagtat taaaccagcc gaagaaaccg cccttatttt
     7321 cgtgatgctc agcagagacg ggtttaagca gcgttgcaca taacgcaggg gtaagaatca
     7381 atgccaccag aacagaaagc gccattgccg aaacgatggt gatagagaac tggcgataaa
     7441 ttgccccagt agaaccgccg aagaatgcca tcggaataaa taccgctgac agcaccatcg
     7501 cgatacccac cagtgcgccc tggatttgcg acattgattt ttccgtcgct tcttttggcg
     7561 ggagcttatc ctccatcatc acgcgctcga cgttctccac caccactatc gcatcatcga
     7621 cgagcagccc gatggcaagc accatcccga acatcgttag tgtgttgatg gagtaaccaa
     7681 aagcggcgag gatggcaaac gtccctaata acaccacggg taccgcaatg gtggggatca
     7741 gcgttgctcg catattctgc aagaacagat acatcaccag gaacaccagc ataatggctt
     7801 caaacagcgt ttttaccact tcgtgaatag aaagctggac gaatggcgtg gtgtcataag
     7861 ggtagagaac cttcattccc tgcgggaaga atggctgtaa ttccgccagt tttgccttaa
     7921 tggctttcgc ggtatcgaga gcattcgcgc cggttgccag cttaatcccc aggcccgccg
     7981 ccggttttcc gttgatacga gcgataacgt tatagttttc accgccaagt tcaactcgtg
     8041 cgacatcttt caggcgtacc accgagccgt cactgtttac gcgcagggtt actttgccga
     8101 attcttccgg attttttaac cgcgtctgag caataattga ggcgttcaac tgttgccctg
     8161 gtaacgctgg cgttccgccc aactgtccgg cagcgatctg atcgttctgt accttcaact
     8221 ggttaatcac atcaaccggt gtcagtttat atttgtttag cagatcggcg tccagccaga
     8281 tacgcatcgc atactgtgcg ccgaaaagct gtacgtcacc gacgccattc agacggctaa
     8341 gcgtatcttt aacgttagag gccacatagt ccgagatatc gtcctgtgtg gtgtctgggt
     8401 tatcagagac aaagcccgcc accatcaaat agctgctact ggacttttca acactgatcc
     8461 cctgctgctg aacctcctgc ggcagcaacg gcgtggcgag ctggagtttg ttctgcacct
     8521 gcacttgcgc gatatcagga tcggtcccgg actggaaggt aagggtaatt gtcacgctac
     8581 cggcggaatc gctggtggag gacatataca tcaggttatc gataccgttc atattctgtt
     8641 cgataacctg cgtcaccgta tcctgcacgg tctgcgcatc agcgcccgga tagtttgctg
     8701 aaacagaaac cgcaggcggt gcaatggttg gatactgagc gacgggtaat tgtaggatcg
     8761 ccagtgcgcc cgccatcatc agaataatgg ccagcaccca tgcaaatatc ggtcgtcgaa
     8821 taaaaaagtt tgccatgtca gattacctta cttcgatgca gtatctgcgg gggtatcggt
     8881 agtggcttta acctgctctc ccggacgcgc tttttgcagg ccgctgacaa tgacttgatc
     8941 gccagatttc agaccttcgc taatcaacca tttatcgcca atcgcctgac tggcaacgac
     9001 agggcgcgct tccacctgac ttttatcgtt aacaatcagc acggttgcat caccacgcgg
     9061 tgtgcggcta acgccttgtt gcgggataag aatggcgtca ggttggacgc cttcatcaat
     9121 ccgtgcgcgc acaaacatac ccggcaaaag cgtatgttgc gggttaggga agacggcacg
     9181 tagggttatg gagccggtgc tttcatcaac ggtcacatca gagaattgca gcgtaccttt
     9241 caggggatag gtttgaccgt tttccatgac caactctacg ttgctggtgg cgttttcctt
     9301 atgcaaattt ccttgctcta cggattgctt cagcctcata aaatcgttgc tggattgggt
     9361 cacatcaacg tagataggat cgagctgctg gacagtcgcc agttcagtcg tttgcccatt
     9421 agtgacaaga gcgccttcgg tcacggtcga tttgccgata cgtccgctaa ttggcgcagt
     9481 cactttggta taagcgagat tgatgcgagc gctttcgact gtggcttttg cggcaatcac
     9541 ggcggcatcg gcctggcgag catcagcaat ggcctggtcg tactcctgct ggctgatgta
     9601 tttcgtaccc acgagcggaa cgtaacgttt taccgtcaaa tgcgcgatgg cggcggcggc
     9661 ttcacttttc gccagttcgc ctttcgcgct gtcataattt gcctgatagg tcgcgggatc
     9721 gatctggtac agggactggc ctgcttgtac atcgctgcct tcagtgaaat tacgattcag
     9781 tacgatcccg ctaacctgtg ggcgaacttc ggctatacgg taagcattgg tgcggcctgg
     9841 taattcagtc ttaacttcta acggggccgt tttcacaata tgaacggtaa cctgcggttc
     9901 accgacgtga gctttctctt ctcccttatc gttacaaccg gcgattaaag ccgcggagat
     9961 cagaataaag gagggcagga ggaaaaacct ggcatgtttc gtcattacta ttcctcaaaa
    10021 aaccaaaagc gcgttattta cccagaaagg caacgcgtta actcgcagaa agaaaaatac
    10081 agtttgctat cctacaaatt atcattcgtc gatgtaagga atagttatga atactggcgt
    10141 ctcaaggcac ataaacacaa aaaaagatta atat
//
LOCUS       DAZFYC010000159        10072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFYC010000159 DAZFYC010000000
VERSION     DAZFYC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.159"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(2..619)
                     /gene="yncG"
                     /locus_tag="ACHKW5_004562"
     CDS             complement(2..619)
                     /gene="yncG"
                     /locus_tag="ACHKW5_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO7513796.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            886..2385
                     /gene="ansP"
                     /locus_tag="ACHKW5_004563"
     CDS             886..2385
                     /gene="ansP"
                     /locus_tag="ACHKW5_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO7513797.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2498..3559)
                     /gene="yncE"
                     /locus_tag="ACHKW5_004564"
     CDS             complement(2498..3559)
                     /gene="yncE"
                     /locus_tag="ACHKW5_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO7513798.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3801..5903
                     /gene="pqqU"
                     /locus_tag="ACHKW5_004565"
     CDS             3801..5903
                     /gene="pqqU"
                     /locus_tag="ACHKW5_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO7513799.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATSRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5939..6604)
                     /gene="mcbR"
                     /locus_tag="ACHKW5_004566"
     CDS             complement(5939..6604)
                     /gene="mcbR"
                     /locus_tag="ACHKW5_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO7513800.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6802..7839)
                     /gene="curA"
                     /locus_tag="ACHKW5_004567"
     CDS             complement(6802..7839)
                     /gene="curA"
                     /locus_tag="ACHKW5_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO7513801.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8023..8541
                     /gene="mddA"
                     /locus_tag="ACHKW5_004568"
     CDS             8023..8541
                     /gene="mddA"
                     /locus_tag="ACHKW5_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO7513802.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8538..8987
                     /gene="ydcZ"
                     /locus_tag="ACHKW5_004569"
     CDS             8538..8987
                     /gene="ydcZ"
                     /locus_tag="ACHKW5_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7513803.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8988..9221)
                     /gene="ydcY"
                     /locus_tag="ACHKW5_004570"
     CDS             complement(8988..9221)
                     /gene="ydcY"
                     /locus_tag="ACHKW5_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO7513804.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9307..9480)
                     /gene="ortT"
                     /locus_tag="ACHKW5_004571"
     CDS             complement(9307..9480)
                     /gene="ortT"
                     /locus_tag="ACHKW5_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO7513805.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9675..9770
                     /gene="yncL"
                     /locus_tag="ACHKW5_004572"
     CDS             9675..9770
                     /gene="yncL"
                     /locus_tag="ACHKW5_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO7513806.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2442 a         2450 c         2455 g         2725 t
ORIGIN      
        1 ctcaaataat ttcattcctt tttaatactt cttgtaattt cggtaactga catacagcat
       61 cagcaatcgc actgatattc gtggcattat cctgaaacca ttcatgccca ggcccccatg
      121 tgcgcatagt gcaaagataa cagtccacca gcgttagttg ctcaccaaac gcataaggtt
      181 cagcggtcaa ttgtgaattc agccagatat aaagcgattt acgatattca ataacattct
      241 tctttaactg ttcaggtgcg tcaggagccc aacgctcagg gtaatcggcg aaagtgaatg
      301 ttggatagac attagcaacc agccagacca atagccgttg aaactgctga cgttcggcgc
      361 gcccaaccgg tggcgcaagg tccgggcgac gatcgagaac catcaatgca atcgccgctg
      421 tctccgtcat aatctcgtca ttttccagcg ccagtgtcgg aatctgacac aacgggttca
      481 gggttttcaa taactcgcgt gaagttccct cgtggtcaaa accactgaca tcaacgaact
      541 gataaggaat atcagccagt gttagcatca gctcactgat tgtagagccc catccgggta
      601 cgccatacac cttaatcata tgcccctcac aatcttttaa agattaagtg taggacagta
      661 cacaggttaa ccagcaaaag gatttgtcat tttttcgtca gttgtattaa tccccttcag
      721 gattgtaagg ggatgttttg catcgtttat cgataacggg taggatgctg cgccgatagt
      781 tttataccca taaagaataa tggtgataac tatcatcgcc aggatgaata aacattgttc
      841 atggcaactt atatgacttt ttcattaaag caatcaggga gagcaatgag taaacacgac
      901 accgacactt cagatcaaca cgccgcgaaa cgccgctggc ttaatgccca cgaagagggg
      961 tatcacaaag cgatgggcaa tcgccaggta cagatgatcg ccattggcgg cgcgattggc
     1021 accggcttgt ttttaggtgc aggagcccga ctgcaaatgg cgggtccagc actggcactg
     1081 gtttatttaa tttgtggctt gttttcgttt tttattctgc gtgcattggg tgagctggtg
     1141 ctacaccgcc cttccagtgg cagttttgtc tcttatgccc gtgagttttt gggtgagaaa
     1201 gccgcttatg ttgctggctg gatgtacttc atcaactggg cgatgacggg gattgttgat
     1261 attaccgccg tcgctctgta tatgcattac tggggtgcgt ttggcggcgt gccgcagtgg
     1321 gtctttgcgc tcgctgcgct gaccatcgtt ggcaccatga atatgatcgg tgtgaaatgg
     1381 tttgcggaga tggagttctg gtttgcgctt attaaagtgc tcgccattgt gacctttttg
     1441 gtcgtgggta cagtgttcct cggtagcggt cagccgctgg atggcaacac cactggcttt
     1501 catttaatca ccgataatgg cggcttcttc ccccacggtt tgctgcctgc gctggtgttg
     1561 attcagggcg tagtgtttgc ttttgcctcc attgaaatgg tgggtacagc tgccggagaa
     1621 tgtaaagatc cgcagaccat ggtgcctaaa gcgattaaca gtgtgatttg gcgtattggc
     1681 ctgttttacg tcggctccgt ggtgttgctg gttatgttat tgccgtggag cgcgtatcag
     1741 gcggggcaaa gtccgttcgt gacgtttttc tctaaactgg gtgtgccata tatcggcagc
     1801 attatgaaca ttgtggtgct gaccgctgcc ctctccagcc tgaactcagg cctgtactgc
     1861 accgggcgta ttctgcgctc gatggcgatg ggcggttccg caccgagttt tatggcgaaa
     1921 atgagtcgtc agcatgtgcc gtatgccggg attctggcga cactggttgt gtatgtcgtc
     1981 ggcgtattcc tcaactatct ggtgccgtcg cgcgtatttg agattgtgtt gaacttcgcg
     2041 tcgctgggaa tcatcgcttc atgggcgttt atcatcgtgt gccagatgcg cctgcgtaaa
     2101 gcaattaaac aaggcaaagc agcggatgtc agttttaaac tgcctggcgc gcccttcact
     2161 tcctggctga cattactgtt tttactgagt gtcctggtgc tgatggcgtt cgattacccg
     2221 aacgggactt atactatcgc ggcgctgccg attatcggta ttctgctggt tataggctgg
     2281 tttggtgtgc gcaaacgcgt tgctgaaatt cacagcactg cgccagtcgt cgaggaagat
     2341 gaagaaaaac aggaaattgt gtttaagcct gaaacggcga gttaattgca aaattgcccg
     2401 gctacgtgcc gggcaaattc agattactga cgaaggtgcc ggatgcggcg tgataaaagt
     2461 caggcaagtc tctccgtttt caccctcgcc atatctatta cagcgcaata cgaatcacat
     2521 cgtctggctg ggtagcttcc tgctgtttag tggatttttg tttcacactg acatacagcg
     2581 ttttgccatc ggcagacagc gccaggctgt ttggatgagt cggcgtatcg aacgttttca
     2641 ccactttata gcttttcgcg tcaatcacac taactttacc tgcctgacga tgcgttacgt
     2701 aggcttcatt acgcgctggg ttaaacagca cagccagtga ttccggtgcc gcaaccttcg
     2761 ccagaatatt gccattacgg gtatcgacca ctaacacttc tgcggcttta gaatcggtga
     2821 taaatgcacg ctgcctggcg gtatcaaggc taatgttgat aaagaagtgc tctttgccgt
     2881 catccagcag ctttttacgg ctgaggattt tattgtcggc ggtgtcgatg gtaatcaatt
     2941 cgccgtcagc gttagtggtg taaagacgtt tgcctttgct atccagcgcc agaccggtac
     3001 tcattttacc ggtgttctgg atggcggttt tcagtttgat attctcgcca tcaacgaccc
     3061 aaatcacgct atctttacca ataccactga tgtaaacggt gttcgtggca tcatcagcta
     3121 ccagctcacg cggttgcagc gggcgcacct cttccgtgcg cttacgatca tccagcacca
     3181 gacggccttt cacttcgccc gttttggcat ctatcgccgt gaccgcgctg tttacggtgt
     3241 taccaaacca caacgtctga gtcgtgttat tgatggtggc accaaacggc ttgagatcgt
     3301 tatggatcgc ctgcgtcact tccagagtga ccggatcaag acgataaacc acgccacctt
     3361 tatccagttt gcggctttgc gaagtggcga gccacagcgc gttttcttgc tggctataag
     3421 ccatttcgta ggcaccttta cctaccgctt tacgcagcat ttcttctgcg gcctgcgtac
     3481 tgaatgatga agcaacaagc aatgaaccta acagtaatga accacgcagg cgcgatgaaa
     3541 acagatgacg taaatgcatg acgactccct ttgatgaacc aatatttttg ctgcttcaca
     3601 atgcttcgct aagcgtaatc atcgttacgc tcttgttata aatgggaatg gtaatcatta
     3661 ttttctcaaa tgtggagcat ttcttgccgt tacgactaca attttctcaa ttaattaacg
     3721 tatcgtgccg tttcgttttc cataaattta caattttatt accatgacag cgttttccca
     3781 ttcttaacct ggcttttttc atgaagattt tttccgtccg acagaccgtt ttgcccgcac
     3841 tgcttgtcct ttcccccgtt gtttttgccg ctgatgaaca gactatgatt gtcagtgccg
     3901 caccgcaggt ggtttcagaa ctggataccc cagcagcagt aagcgtggtg gatggcgagg
     3961 agatgcgcct ggcaacatcg cgcattaact tgtccgaatc actgaccggc gtgcctggtt
     4021 tgcaggtaca aaaccggcag aactatgcgc aagatttaca gctgtcgatt cgcggatttg
     4081 gctcccgctc cacttacggt attcgcggta ttcgcctgta tgtggacggt attcccgcca
     4141 ccatgcccga cgggcaaggg caaacatcca acatcgattt aagcagtgtg caaaatgtgg
     4201 aagtgctgcg tggccccttc tctgccctgt atggcaacgc gtctggtggg gtaatgaatg
     4261 tcaccaccca gaccggacaa cagccaccaa ccattgaagc cagtagttac tacggcagtt
     4321 ttggcagctg gcgctatggg ctgaaagcaa cgggcgcaac gggagacggc acacagcctg
     4381 gcgatgtcga ttacaccgtc tcaaccacgc gttttacgac ccacggctat cgtgaccata
     4441 gtggcgcaca gaaaaattta gctaatgcca aactgggcgt acgcattgat gaagccagca
     4501 aattaagtct gattttcaat agtgtggata tcaaagcaga tgacccaggt gggctaacca
     4561 aagcagaatg gaaggctaat ccacaacaag cgcctcgtgc agaacagtac gatacgcgaa
     4621 aaaccatcaa gcaaactcag gctgggttgc gctatgagcg tagcctgagc gcgcaagatg
     4681 atatgagtgt gatgatgtat gccggagagc gagaaacgac ccagtaccag tcaataccca
     4741 tggcaccaca acttaacccg tcacatgcgg gcggcgtgat taccctgcaa cgccattacc
     4801 aggggataga cagccgctgg acacaccgtg gtgaactggg cgttccggtc acgttcacta
     4861 ccggcctgaa ctacgaaaac atgagtgaaa accgcaaggg ctacaataac ttccgcctga
     4921 atagcggcat gccggagtac gggcaaaaag gtgagttgcg tcgcgacgaa cgcaatctga
     4981 tgtggaacat cgatccctat ttacagacgc aatggcagct gagcgaaaaa ctgtcgctgg
     5041 atgctggcgt gcgctacagc tccgtgtggt ttgattccaa cgaccattac gttactccgg
     5101 gtaacggcga tgacagcggt gatgccagtt atcataaatg gctacctgcc ggttcgttaa
     5161 aatatgcaat gaccgatgcc tggaatatct atctggcagc cgggcgaggt tttgaaacgc
     5221 cgacgattaa tgagctgtct tatcgtgctg atgggcaaag cggtatgaac tttggtttaa
     5281 aaccatccac caacgataca attgagatcg gcagtaaaac gcgtattggt gatgggctgc
     5341 ttagtctcgc attgtttcag accgacactg atgatgaaat tgttgtcgat agcagtagcg
     5401 gtgggcgtac gacttacaaa aatgccggaa agacccgtcg tcaaggcgct gaactggcat
     5461 gggatcaacg tttcgcagga gattttcgcg taaacgcgtc ctggacctgg cttgatgcga
     5521 cctatcgcag caatgtttgc aatgaacagg attgtaacgg taatcggatg ccagggatcg
     5581 cccgtaatat gggctttgcg tcgataggtt atgtaccgga agatggttgg tatgcaggca
     5641 cggaagcgcg ttatatgggc gatattatgg cagatgatga aaatacggca aaagcgccgt
     5701 cttatactct cgtcggctta ttcaccgggt ataaatacaa ttaccacaat ttaactgtgg
     5761 atttatttgg tcgtgtcgat aatttattcg ataaagaata cgttggttct gtcattgtca
     5821 atgagtcaaa cgggcgatat tacgaacctt cgcccggacg aaattatggt gtcggcatga
     5881 atattgcgtg gagatttgag taattacctt gatgcccggt atttgccggg catttacttt
     5941 aacgattgta ttgctggtat aaaatagcaa tattttgttg cataatttca gcaagacaat
     6001 gtctgcatcc ctctttgtct tttgctttta acgcagcgag taacagatga tagttttcta
     6061 tatgctcccg taattccgct ggattaatcg cttcatagag ataatgtaaa ccaggcccca
     6121 tcctgaccca cagttgctca atcatctcac acaggatggg catatttgag cgatgataaa
     6181 tcgctaagcg aaatagcctg tttacattaa tgatttgttc catgtcgccc ttttcttgcg
     6241 cctgctgtaa tttctcgagc aattcctgga gttccgccag gtcttgcggg gtgaggtttt
     6301 caacagccag agcaactgcc attaattcca gttcgtagcg gatccgattg atttcgtcta
     6361 attgacgttt cctcacttcc ggaactgtaa atgcttgtgc aggtgcgaca gaaagcgcat
     6421 tcaccgaaac cagacgtaat aatgcttcac gcacaggtgt aatactcata cctaattgct
     6481 ccgccagatt tttagtaatc aggcgtgcgc caggttttaa tgcgccaata ctaagctgat
     6541 gtttcaggtc gttctcaacc tgcagagtca aactgacatg tttcattttt cccattccag
     6601 gcattttaat tccttcgcgt gttttctgtc caaccctgcc tgcacggcag aaggagttca
     6661 cctggtgtat taaagtgaat aatcataaac cacccacctt tgttgttatt agcagcagaa
     6721 caatgacgtg gttagttaat taaagctgaa aataagcttt ttgcctggat ttttatggcg
     6781 gcttacgccg ccgttgaaac tttaatcatc acccgcgacg cggatcacca ctttgccgaa
     6841 gtttttaccc ttcagcaggc cgataaacgt ctgtggcgca ttctctaaac catcagtaat
     6901 atcttcgtgg tagtggattt tatcctcttt cacccattgc cccatctcct tctgaaactc
     6961 atggatgcgg tgaccataat cctgagcgat aataaaccct tgcaagcgaa tacgtttttt
     7021 cagcaccgtt gccatcaaca ggggtaaacg atccggaccg ggtggtagct ctgtagcgtt
     7081 atagctgctc actaagccac aaacgggaat gcgcgcagat gtattaagta atggtagcac
     7141 tgcatcaaat accttaccgc ccacgttttc ataatagata tcaatacctt ttgggcacgc
     7201 tttcaccagt tgttcggcaa aatcatccgc gtggtgatca agacaaacat cgaagcccag
     7261 cacctcgata gcatggcggc atttttccgc gccaccggca acccccacca ccctgcaacc
     7321 tttaagtttg ccgatttgcc ccaccgttgc tcccacagga cctgtcgccg cagctaccac
     7381 caacgtttcg ccctctttag gctgaccgat atccagcagc cccatataag cggtaaagcc
     7441 tggcatcccc agcacaccca gcgaccacga tggattttgc ggatgatcgc caagtttcac
     7501 cagatcatca ccactggata tgtcatagtc ctgccatcca ctgtagccca gcacccagtc
     7561 gccaggctga taatcaggat gattcgactc cacaacacgg ctcaccgtgc cgccgaccat
     7621 cacgccgcca atatcaacag gcggtgaata agatggctca tcgctcatac gtccacgcat
     7681 atacgggtcc agggacaaat aaactgtgcg cagtaacacc tgtccttcac ccggtgtggc
     7741 gacatcatct tcttcaagac ggaaattctc cggaaccggt gcgccatgtg gacgcgaggc
     7801 cagaacccaa cggcggttac gctgcttttg ttgccccata atgctctcct gtgcttttgg
     7861 catgaatcca tcaatagtag caggctcgca ggattggttt tgtttgcagc cgtcacatcc
     7921 cctctgcaac acgcaataat ttcatcgagg aatccgttgc ttcgggctgt tttcgatagt
     7981 attgataaca tcatcatcat cattatttta ttgaggccag acatgtccat ccgttttgcc
     8041 cgcaaagccg actgtgctgc cattgcggaa atttataacc acgccgtgtt gtatacggcg
     8101 gctatctgga atgaccaaac ggtggatgca gataatcgca ttgcctggtt tgaagcgcgg
     8161 accatagcag gttatccggt gctggtaagt gaagaagata gcgtagtgac gggatatgcc
     8221 tcgtttggcg actggcgtag tttcgatggt tttcgccata ccgtggaaca ttcggtttat
     8281 gtccatcccg atcatcaggg caaaggtctg gggcgtaaat tgttaagccg attgattgat
     8341 gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca aaatcaggcc
     8401 tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg cacaaatgcc gcaggtgggc
     8461 actaaatttg ggcgctggct ggatctgaca tttatgcagt tgcaactcga cgaacgcact
     8521 gaaccggacg cgattggatg aatcagtcgc tcacccttgc ttttttgatt gccgccggaa
     8581 ttggtctggt agtccagaac acgctgatgg tgcgcatcac ccagacctcc tctaccattc
     8641 tcatcgccat gttgctgaac tcactggtgg ggattgtgct ctttgtttct attttatggt
     8701 ttaaacaagg catggcgggg tttggcgaac tggtgtccag cgtgcgctgg tggacactta
     8761 tacccggctt gctgggatcg ttttttgtct tcgccagtat cagcggatat cagaatgtgg
     8821 gagcggcgac aactattgca gtactggtcg ccagccagct aattggcggt ctgatgctgg
     8881 atatcttccg cagtcatggc gtaccactac gggcgttgtt cggcccaatt tgtggcgcaa
     8941 ttttacttgt ggttggcgcc tggttggtag ccagacgctc attttaatca gaggatggtg
     9001 ccgcctttgg ttaactgttc gtggcgcgct tccatatctt ctttatgctt gcgaccgtga
     9061 tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg acttaactcc
     9121 gcgtcatcgc tcagggcgat taataattcg ttcatatggg caatgacgct ctcttcaatc
     9181 gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca tactttctcc ttaaccactt
     9241 gtgaaattta tcgattcatc caaatgatat aagtctgcgt ggtgccgtca agccagtgag
     9301 taagatttaa aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc atgttgcccc
     9361 caccagaaag gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa accaggtgat
     9421 aagaaatgcg attgctgcca taaccgcata aaaaacaagc atatgttgat agagagacat
     9481 gtacgggcgt ccagccagat gaataaaacg cgcgcaaata tgcgtcattt tttgacgtgc
     9541 atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc cttatccatc
     9601 ttcagaatga gtattattgc ggattttctt aacccgtact atacacatag gggcaatgat
     9661 aaaaggtggc aaaaatgaat gtttccagta gaactgtagt actgataaat gtctttgctg
     9721 ctgttggttt gtttactctt atctctatga gatttggctg gtttatttga tgtagtgatt
     9781 ttgcacgcag cacatactgt cagagaaatc aactctctga cagtacgcaa gatcttctca
     9841 tactctccag gtttacaaca ttatccttaa tatttacttc tttttgctgg catctacatt
     9901 gaaatgagta gaaaacatcc ccctactgtc gtctatacaa acagcttact gattcaaacg
     9961 gaatcttgtt ttcacatttt tctatcatga atactttata cgcgcgggag tctataaatg
    10021 ctttatactt tcaaggaata acgtaaatat aaaaagatgg cgtaaagtat at
//
LOCUS       DAZFYC010000160         9890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFYC010000160 DAZFYC010000000
VERSION     DAZFYC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.161"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(400..1593)
                     /locus_tag="ACHKW5_004573"
     CDS             complement(400..1593)
                     /locus_tag="ACHKW5_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7513807.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1729..3453
                     /gene="iucA"
                     /locus_tag="ACHKW5_004574"
     CDS             1729..3453
                     /gene="iucA"
                     /locus_tag="ACHKW5_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO7513808.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3454..4401
                     /gene="iucB"
                     /locus_tag="ACHKW5_004575"
     CDS             3454..4401
                     /gene="iucB"
                     /locus_tag="ACHKW5_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO7513809.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4401..6143
                     /gene="iucC"
                     /locus_tag="ACHKW5_004576"
     CDS             4401..6143
                     /gene="iucC"
                     /locus_tag="ACHKW5_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO7513810.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6140..7477
                     /gene="iucD"
                     /locus_tag="ACHKW5_004577"
     CDS             6140..7477
                     /gene="iucD"
                     /locus_tag="ACHKW5_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFO7513811.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7483..9678
                     /gene="iutA"
                     /locus_tag="ACHKW5_004578"
     CDS             7483..9678
                     /gene="iutA"
                     /locus_tag="ACHKW5_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO7513812.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2337 a         2581 c         2625 g         2347 t
ORIGIN      
        1 tccagctctt tcaggtgtca acgccactct gagagagttt tatgcatccc gtattgggca
       61 ctatgatgcc ggaaatctgc gaggctcgac acctctctga ttttcccctt tatttatcac
      121 ggtgccccag atctaatgtt ggggatgcgt tcatctcatt cccaatgagt aatcgcactg
      181 ttctcattgt ctgcaggcat ccggacagag ccatgtgatg ttgcactcac aaccagcatg
      241 acttcaatgc aacttaagtg acaccggccg gttttatacc gaacataccc tgtaccatgc
      301 ggtgcatttt tgccagtgtt actgcctgac atgctttcat cagacgcagg ttctctctca
      361 tatcctctga gttactccgg taaggtgatg atcatatctt tatttgatag cacggtaatg
      421 ccagataaat acagacacaa tggtaaataa tatagttgca aataatgccg ccataatcag
      481 cgcgattgcg acacctgttt tcgataccag cggcaccagc aaagcgcaca gtccatagcc
      541 aagtgtgtga ctggtagcga gccagcccgc gcctgcgcca tcagccagct tatcattcag
      601 tagaagttga taagcgggtg ttgccagagc tgctccaaat gacagcactg cacagcccgg
      661 gtaaaacaac catagctgtt cggagagcat gatagccaga ccaccactca tcaacactcc
      721 ggcactcagg agcagggcca ccggagtcag gcgctgcgga cggagtaccc cgaactgcgc
      781 gataagcgca gctactgcgg acagacccaa cagccaggcc acctgttggc taatggcggt
      841 ggtatccgtg gcgaactggc gagtaagggc aggcgaaagt ccaagctgca tcatgctcac
      901 cgccgcagcc agtaatattg cgcaaagcag ataaggcaga caatcccgct tcagactgac
      961 gctcttgcgc tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagca gcatcagcag
     1021 cgccagcact ggggctgcca tcagcagccc cagtggcgcc agaggatgaa tggccagcat
     1081 tgctgccgcg cacagcggcc cgaatagcct gccgcaactc aggccggagc tgatggttgc
     1141 cagagcggcc atgcgattcc cttctcctgc tctctgcaat gcccagacct gacaggctgg
     1201 caccattgcc gacacggtca gcccgtaggc gatccgggcg atgattaata ttcccagtcc
     1261 caccgctgtt gttatggcgc tggtagccat cagcacgctt cccagcccta gcagggtaaa
     1321 gcttatgcca tatcctccca gagcccacaa caccacaggt ttactgccag tacgggaaat
     1381 ttgctttccc caccacgggg acgatggcag aaacagcatt gagccaagca tcagcagggc
     1441 agcccataca gacagactca gatttgtctg tatgaccaga acagggagca caaccagcag
     1501 accgttctgc ccgataccga gaagcccggc actgaacgca agtggccagc aggacagtgg
     1561 tttttggggc gtatcttcga tcccaggtga cattttaatg tttcaactcc atgtattaat
     1621 tgtgtttatt tgtaaaatta atttatctga caataacatt tcttattgat aatgagaatc
     1681 attattgaca taattgttat tattttactg tgtgggagct gtttgactat gaccctgccc
     1741 tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact gattcgtgaa
     1801 accacagact ggaaactgac agaatacccg ccagacgaat tgatcatccc gctggatgag
     1861 cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca ccgctttgca
     1921 tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac cactctctcc
     1981 cggcttatta ttgataagct acggcatcaa ctgtttctgc ccgttcccct ctgcgaaact
     2041 ttccaccagc gcgtgctgga aagccacgcc catacgcaac aggcaattga tgcccgtcat
     2101 gactggaccg ccctgcgtga aaaagcgttg aattttggcg aagctgagca ggcgctgctg
     2161 acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg gcgggaggct
     2221 gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc ggtggataaa
     2281 acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac gcgatttgcc
     2341 gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct gttcccgttg
     2401 cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact tgttgctaag
     2461 gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac ctcttcccgt
     2521 tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt acggctgacc
     2581 aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct ggcacgcctg
     2641 gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg ggtaatgcag
     2701 gaggatggct gggccgggct gctcgatctt aacggcaaca tcatgcagga aagtctgttt
     2761 gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agaccaacgt actggtctcc
     2821 ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt aaaacgcctg
     2881 agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggtcga tgcatactgt
     2941 cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt gctgctggcg
     3001 catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt tatttaccgt
     3061 gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc tattggcgag
     3121 gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc ttattacctg
     3181 ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct ggacagcgaa
     3241 tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca ggtgactcat
     3301 aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg taactttttc
     3361 tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat ctatttcgat
     3421 ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat tattcacagc
     3481 agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg gggactggac
     3541 aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg cgatgcgctg
     3601 gatcagatat ttatcgccgc accacaactg tcagcagtgg ctctcccctg ggccgaatgg
     3661 tgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat tatccatcgt
     3721 accgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc ctccggtgaa
     3781 atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg tccgcagggt
     3841 gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg cattgccgat
     3901 cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt tgagtatttc
     3961 tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca gctcaccggt
     4021 aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta ttttgaaatc
     4081 tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt tgaccgtggc
     4141 ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca aagctggctg
     4201 cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac agtactggag
     4261 ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata ccggacaatt
     4321 aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg tcatcacttc
     4381 ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac cgtcggctgg
     4441 tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa tctcagggcg
     4501 atgaccacta ctgcattaac ctgccgggag cacaatggcg ctttatcgct gaacgtggta
     4561 tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa ccagtactgg
     4621 cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc actgttgcag
     4681 agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg aaggcccgtc
     4741 gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa tgcctgctga
     4801 gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa gaggcgctgg
     4861 aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg gtaaaacgtg
     4921 aacatatgat ctggcgctgc gacaacgatc tggatattca gcagttgttg actgccgcaa
     4981 tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacggg ctggatcaca
     5041 actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgcc accgacttca
     5101 tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac cagtggctcg
     5161 cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg gggagggctg gatatcaagc
     5221 tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga tatatcgctg
     5281 ccggaccact ggcttcacgc tggctgcaac aggtttttgc gaccgacgcc accctggtgc
     5341 aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta tgtgtcccat gaaggctatg
     5401 ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc tggcgggaga
     5461 atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca ctgatggagt
     5521 gcgacgaaaa caatcagccg ctggcaggcg catatatcga ccgctccgga ctggacgctg
     5581 aaacctggct tacgcaattg ttccgggtgg ttgtggttcc gctgtatcac ctgctttgcc
     5641 gctacggtgt cgcgcttatt gcacacggac aaaatataac gctcgccatg aaagaggggg
     5701 taccacagcg tgttctgctg aaagacttcc agggcgatat gcggctggtg aaagaagcgt
     5761 ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt cacatcccgt ctgagtgcgg
     5821 actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt tttatttcgc
     5881 cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg gcagcagtgt
     5941 tgagtgatta catgaacaaa catccacaaa tgtcagagcg ttttgcgctt ttctcactct
     6001 tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg ccggaccagg
     6061 atggcggcag tcgcatgctg ccgaattacc ttgagaatct gcaaaatccg ctgtggctgg
     6121 taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtcg ggacagggcc
     6181 atttaatctc agtattgctg cattgtcaca tcagatcgaa gaactgaact gtctcttctt
     6241 tgacgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt gtcatatgca
     6301 gactgtcttt ctgaaagatc tggtcagtgc agttgcacct acaaatccct acagctttgt
     6361 taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac tacgtacagt
     6421 atcccgtgaa gagttttctg actatctccg ctgggctgct gaagatatga ataacctgta
     6481 tttcagtcat accgttgaaa atattgattt cgacaaaaaa agtcgattgt ttctggttca
     6541 gaccagtcgg ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa aacaacctta
     6601 tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc catgccagtg aaatgaatct
     6661 tcgtcggccg gacctgagtg gaaaacggat aactgtggtt ggtggaggac agagtggtgc
     6721 agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa taaactgggt
     6781 ctcacggcgt aataatttta acgcactgga tgaggctgct tttgctgatg agtattttac
     6841 acctgaatat atttcaggct tctccggact gaaggaagat attcgccatc agttactgga
     6901 tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca tttatcgtga
     6961 gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc tacccagccg
     7021 ctcggtaaca actctggaaa gtagtggtcc gggctggaag ttattgatgg aacatcatct
     7081 ggatcggggc agagagagcc tggaaagtga tgtggtgatt ttcgccacag gttaccgttc
     7141 tgcattgcca caaatacttc cctcactgat gcccttgatc accatgcacg ataagaacac
     7201 ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaaa ataacatctt
     7261 tgcggtcaac gccagcatgc aaactcatgg catcgccgaa ccccagctca gcctgatggc
     7321 ctggagatct gcacgtattc ttaatcgcgt actggggcgt gatttattcg atctcagtat
     7381 gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa aaaccgcagc cggaggctgc
     7441 tgctttaact cactatacaa caaatattca ggaataacga tgatgcgcaa aaagtatatg
     7501 ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac cagctgtcgc ccagcaaaac
     7561 gatgataatg agatcatagt gtctgccagc cgcagcaatc gaactgtagc ggagatggcg
     7621 caaaccacct gggttatcga aaatgccgaa ctggagcagc agattcaggg cggtaaagag
     7681 ctgaaagacg cactggctca gttaatcccc ggccttgatg tcagcagcca gagccgaacc
     7741 aactacggta tgaacatgcg tggccgcccg ctggttgtcc tgattgacgg tgtgcgcctc
     7801 aactcttcac gttccgacag ccgacaactg gactctgtcg atccttttaa tatcgaccat
     7861 attgaagtga tctccggcgc gacggccctg tacggtggcg ggagtaccgg agggttgatc
     7921 aacatcgtga ccaaaaaagg ccagccggaa accatgatgg agtttgaggc tggcacaaaa
     7981 agtggcttta acagcagtaa agatcacgat gagcgcattg ccggtgctgt ctccggcgga
     8041 aatgaccata tctccggacg tctttccgtg gcatatcaga aatttggcgg ctggtttgac
     8101 ggtaacggcg atgccaccct gcttgataac acccagaccg gcctgcagca ctccaatcgg
     8161 ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct tcaactgata
     8221 acgcagtact ataaaagtca gggggacgac aattacgggc ttaatctcgg gaaaggcttt
     8281 tccgccatca gcgggagcag cacaccatac gtcagtaagg ggctgaattc tgaccgcatt
     8341 cccggcactg agcggcattt gatcagcctg cagtactctg acagtgattt cctgagacag
     8401 gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc ggttctaccc gttcccgacg
     8461 gtaaatgcga ataaacaggc gacggctttc tcctcgtcac agcaggatac cgaccagtac
     8521 ggcatgaaac tgactctgaa cagccaactt atggacggct ggcaaatcac ctgggggctg
     8581 gatgctgagc atgagcgctt tacctccaac cagatgttct tcgatctggc tcaggcaagt
     8641 gcttccggag ggctgaacaa ccataagatt tacaccaccg ggcgctatcc gtcatatgac
     8701 atcaccaatc tggcggcctt cctgcaatcc agctatgaca ttaatgatat ttttaccgtc
     8761 agcggtggcg tacgctatca gtatactgag aacagggtag atgattttat cgactacacg
     8821 cagcaacaga agattgctgc cgggaaggcg atatctgccg acgccattcc tggtggttcg
     8881 gtagattacg ataactttct gttcaatgct ggtctgctga tgcacatcac cgaacgtcag
     8941 caggcatggt tcaatttttc ccagggggtg gcattgccgg atccggggaa atattatggt
     9001 cgcggcatct atggtgcagc agtgaacggc catcttcccc tgacaaagag cgtgaacgtc
     9061 agcgacagta agctggaagg cgtgaaagtc gattcttatg aactgggctg gcgctttacc
     9121 ggtgacaacc tgcggactca aatcgcagca tattactcgc tttccaataa gagcgtggaa
     9181 aggaataaag atctgaccat cagtgtgaag gacgacaggc gccgtattta cggcgtggaa
     9241 ggtgcggtgg actacctgat cccggatact gactggagta ccggtgtgaa cttcaatgtg
     9301 ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat atgacgtgaa ggaatcaagt
     9361 ccatcgaaag cgacagctta cattagctgg gcgccggaac cgtggagtct gcgtgtgcag
     9421 agcaccactt ctttcgacgt aagcgatgca gagggtaacg atattaatgg ttacactacc
     9481 gtcgatttta tcagtagttg gctgcttccg gtgggaacac tcagcttcag cgttgagaac
     9541 ctcttcgacc gtgactatac cactgtctgg ggacagcgtg cacctctgta ctacagcccg
     9601 ggttacggcc ctgcttcact gtacgactac aaaggccggg gccgaacctt tggtctgaac
     9661 tactcagtgc tgttctgacg gggattcttt tgcaacaagg gtatgctgta aacaacatgg
     9721 tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa gatattccgt
     9781 ctccggaatg gactgttcac cactgaataa caatatgtct gttttatgga tctttgactg
     9841 aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac
//
LOCUS       DAZFYC010000161         9869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFYC010000161 DAZFYC010000000
VERSION     DAZFYC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.162"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..631)
                     /locus_tag="ACHKW5_004579"
                     /pseudo
     CDS             complement(1..631)
                     /locus_tag="ACHKW5_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1026..1682
                     /locus_tag="ACHKW5_004580"
     CDS             1026..1682
                     /locus_tag="ACHKW5_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HFO7513813.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(1991..2605)
                     /locus_tag="ACHKW5_004581"
     CDS             complement(1991..2605)
                     /locus_tag="ACHKW5_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7513814.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKRE
                     ERPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATID
                     TTTAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMA
                     SMGQSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
     gene            2767..2949
                     /locus_tag="ACHKW5_004582"
                     /pseudo
     CDS             2767..2949
                     /locus_tag="ACHKW5_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            3085..3630
                     /locus_tag="ACHKW5_004583"
     CDS             3085..3630
                     /locus_tag="ACHKW5_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFO7513815.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGI
                     SYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFC
                     ISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQL
                     SRLDLASPVLEPEVIWLGRRR"
     gene            3767..4339
                     /locus_tag="ACHKW5_004584"
     CDS             3767..4339
                     /locus_tag="ACHKW5_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7513816.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            4376..5767
                     /locus_tag="ACHKW5_004585"
     CDS             4376..5767
                     /locus_tag="ACHKW5_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HFO7513817.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLTVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(5913..6749)
                     /locus_tag="ACHKW5_004586"
     CDS             complement(5913..6749)
                     /locus_tag="ACHKW5_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO7513818.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(6749..7552)
                     /locus_tag="ACHKW5_004587"
                     /note="aph(3'')-Ib"
     CDS             complement(6749..7552)
                     /locus_tag="ACHKW5_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO7513819.1"
                     /translation="MNRTNIFFGKSHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(7613..8428)
                     /gene="sul2"
                     /locus_tag="ACHKW5_004588"
     CDS             complement(7613..8428)
                     /gene="sul2"
                     /locus_tag="ACHKW5_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO7513820.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            8758..8844
                     /locus_tag="ACHKW5_004589"
                     /pseudo
     CDS             8758..8844
                     /locus_tag="ACHKW5_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(9116..>9869)
                     /locus_tag="ACHKW5_004590"
     CDS             complement(9116..>9869)
                     /locus_tag="ACHKW5_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HFO7513821.1"
                     /translation="KSNKNDFVDAEAICEAASRPSMRFVQPRTESQQAMRALHRVRES
                     LVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTLLEDSSLPLYLSQLLLKLQQHYH
                     YLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAA
                     ATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRELL
                     CRKSNFVVTCALANKLARIAWALTARQQTYEA"
BASE COUNT         2428 a         2547 c         2517 g         2377 t
ORIGIN      
        1 agcagattat gcgttctcat aatgcgatac acccgctttg cattaaccac aggcaggccg
       61 tcccgctccg actcccgtcg cagcagcgcc cacacacggc gataaccata cgtcggcagg
      121 tcagccaccg ccatgtttat ccgggacaat acggtggtat cgtcagaacg gggctgccgt
      181 ctgcgatcct gtcagtcaga tggccggtga acccgaatgc tcaactgcgc acgcgacacg
      241 ccaagacttc tgcaaacgtc gcttattcgt cgttcccagg caacgagggc gcatgcgcaa
      301 tccatttttt tgcccggccg aactccacgg cctctttgag tatctcggct tccatcgtct
      361 ttttgcccag aaggcgctgg agttcgcgaa tttgcttatt ggcggcagcg agttcggagg
      421 ccggcaccac ttcctcgccc gatgcaacag ccgtcagtga accgtcttaa tattgcttgc
      481 gccatttgaa tatctggttt gcattgatgc catgcaggcg cgcgacatga gacacggtca
      541 taccgggctc catagtctgc tgaataatgg caattttttc ctgcggtgta cgacgccgac
      601 gccgctcagg tcctgataac actccaacca tcttatcgtt ctgactggta ttaaacatag
      661 ttccaagact acctcttatt ttaagagagt cgaagtgtct ggtgatctat ggggccagtc
      721 tagccctctt ccaagtgtag ggagatatag tcaaagtttg cttctccgcc gatgagtccg
      781 ccctgcgtcg gatagtccga ttttttaccg gcgaagcacg gctctcctaa ccctatctaa
      841 agcgaataac ttttttcaac agggccgcgt gccaatgcaa gagcgatagg cgctctacgc
      901 gccaatttga ccacttaaaa caggtaaatt gaggggttgt aatgtgttga tgtaacaggc
      961 ttttatttta atgtcttggc atatgtataa tggtagtcta gccctccttt caacaaggag
     1021 tactcatgga aacctacaat catacatatc ggcaccacaa cttttcacat aaagacttaa
     1081 gtgatctcac cttcaccgct tgcacattca ttcgcagcga ctttcgacgt gctaacttgc
     1141 gtgatacgac attcgtcaac tgcaagttca ttgaacaggg tgatatcgaa ggctgccact
     1201 ttgatgtcgc agatcttcgt gatgcaagtt tccaacaatg ccaacttgcg atggcaaact
     1261 tcagtaatgc caattgctac ggtatagagt tccgtgcgtg tgatttaaaa ggtgccaact
     1321 tttcccgaac aaactttgcc catcaagtga gtaatcgtat gtacttttgc tcagcattta
     1381 tttctggatg taatctttcc tatgccaata tggagagggt ttgtttagaa aaatgtgagt
     1441 tgtttgaaaa tcgctggata ggaacgaacc tagcgggtgc atcactgaaa gagtcagact
     1501 taagtcgagg tgttttttcc gaagatgtct gggggcaatt tagcctacag ggtgccaatt
     1561 tatgccacgc cgaactcgac ggtttagatc cccgcaaagt cgatacatca ggtatcaaaa
     1621 ttgcagcctg gcagcaagaa ctgattctcg aagcactggg tattgttgtt tatcctgact
     1681 aattgctttg atgtgtgatt ttaaacgctc aaatttataa aacgaatacc ttgcaataat
     1741 agacataggg acaaccatgt ataaattttt accctcaggc gtcttcacaa tagagccata
     1801 tagcagtggg tcacttagtg ggcttaattt ttccattaaa gataatatta atattgctca
     1861 gtataaaaca tcatatggta gcccatcttg gcaagtaaac ataaggctgc catttataag
     1921 gcactgttgc aaaaatgtgg aggtgatagg ggtactacgc cggaacccca gatttttccg
     1981 tccgttcaaa ttatccacgg ttgaacagct tgatcccgtc cgcccgcagt tgaccaacag
     2041 gggaaatatg cctgtacaaa gtttgccggg ttatcccaag ttcctggcat agcgtactga
     2101 cctttgtttc tgattgtccc attgatgcca ttgccagtcg cagtttgact ggcgtcattt
     2161 tataaggtcg gccgccgttc cgcccacggg ctcttgctga tgccagacct gcagtcgttc
     2221 tttcagcaat cagttcgcgc tcaaactccg ccagtgcggc aaagataccg aagacaagct
     2281 tgccagcggc cgttgttgtg tcgatagtcg ccccgtgacc ggtcagcact ttcagaccgg
     2341 tccccctcgc ggtcaggtcg tgcactgtat taatgagatg acgaagatca cgaccgaggc
     2401 ggtccagctt ccatacgacc agcgtgtctc cgggtcgtag tgctttcaga caggctgtaa
     2461 gtccaggacg ctcttctcgt ttgcccgatg cccggtcctc ataaaaatgt gcggcatcaa
     2521 cacctgcggc gatcagtgca tcacgttgta aatcattcgt ctgcgaacca tccgcttttg
     2581 acatccgaat atatccgatg aacatatttc ctctgtcaca tatacgttcg attatgtgac
     2641 attctgattt gggtaccata atctgtcaac ggtagtcaca ttacccgtaa tttaaattat
     2701 gacacgtaaa ggttctatgt taatgataat gtgacaagcg cgtttaaata ggcataagga
     2761 tcagagatgc ctactaaaaa taagctcctc agtattcttt cggacgccga gcaggaagcg
     2821 ctctatggtc tacctgattt cgatgatgca cagcggctgg agtttctggc gctgaatgaa
     2881 tatgaactgg cgctggcctg cagtcgccgg gggcttcacg ctcaaattta ttgcatcatc
     2941 cagatcggag agcatgaaaa acctcggggc agccatcgaa tgatgccatc tacaggggtt
     3001 aaaataacca ccatcatttc ataacaatac ctgctgacta tgaagatcta cgtcatcaaa
     3061 atagcagttc atggagtcag tccgatggtc tggcgtcggc tgagaattgc tgccgacacg
     3121 tcactggccg cgcttcactt cattttccag atagtgcagg gctgggggga cgaccatctc
     3181 catcaatttc acatttatgg caaagattac ggtatctcct acgaaggagg catcggtttt
     3241 gtcgataatc cgttccgggt cgtgattgat gattttgctt ttgatgctgg cgatcgcttt
     3301 acctatgaat acaatttctt tgagcactgg cttcatgaca ttcgtgttga ggccatttat
     3361 gaaaactcta cgctgaaagc gccgttttgt ataagcggcc atggtatgcc cggagccaca
     3421 gctgcggatg agttcgataa aaccctggcg tttcttgaag ctatcgttaa tgcggatgat
     3481 gaaacaacgg tcggcgagat ccgccctttt gctgacgacc tggatgctgt caggttcaac
     3541 cgccacaaaa tcaaccggca actgagcagg ctcgaccttg catctccggt actggagcct
     3601 gaagttatct ggctgggccg tcgtcgctga ctgatgtcta taaaggtata cgttttcagc
     3661 atgcctctca gatccccgaa aaaatgtcta taaacctccc taaatcagtg tttatggatt
     3721 attctttaaa tctctatgat gtttaaagac cttccggagg ttttgcgtgc aatatgccta
     3781 catcagggta agctcggccg atcagaatac ggcccgtcag gaagaggcat tgtcaaaggc
     3841 gggttttcag cctgataaaa tttgtgttga gcatgccagc gcgaaggaca caaaccgccc
     3901 ggggttacag gaactcctgg ggcaattacg ccctggtgat acgcttctgg ttcattccat
     3961 cgatcgcctt tgccggaata tgtcggatat gtgcgctgtc accacccggc ttcgtgacca
     4021 gggcgttacc cttatttttc tgaaagagca gctgacgttc agcgccggca caaacaatcc
     4081 gatgcaggaa ctgcagctgc acatgatgtc ggcattcagc cagtttgaac gagccttact
     4141 gaaggaaagg caggccgacg gcatcgccgc gaaaaaagca cggggtgaaa aaacagggcg
     4201 ccccggcgcc gatatcaaaa agatccagga aattgacgca ctcagaagca gaggggtcag
     4261 gctcaggatc gcctgcgacc atgcgggcct tggcgtttcc acatattata agcttcgtca
     4321 ccagatgaac aaccagtagc cgggagatag tcatccacca cagcaggagg caatgatgca
     4381 actgacgctc cagatagtca tcaccgatga atcaggctcc agtcgaacgg aggaactgat
     4441 gacaatccag aaatcagggg agacccggaa cgacatcgga ttatcggtgt cagaatccaa
     4501 gctgttgctg aatacggtcc agcagtcggt ggtccagctg caggcagacg aatatactca
     4561 acaccatatc cggtgccctc actgccttgc tgcgcgcaga atcaaaggca aacagaaaat
     4621 acggtaccgg acgctgtttg gcgttattcc ggtatccggg cttcgggtgt accggtgccg
     4681 ttgtgaagag agtgatacaa agacagtcag tttgctcagc gactgggccg gcgattattc
     4741 tcacccggca ctgaaatata ttgaaacccg ctgggcctcg atgatctcct atgaaatgac
     4801 gactcgtctg ctgaaagata ttctgccggt gggccacagc ctgaatgcct caacggtgag
     4861 gaatcattta tgccaggtgg cgcagcgtct tgacgctgag gctgaagctc attcaggttt
     4921 tctttccggc tgcccccgcg actgggggaa cctgcccagg ccaggaaaac cgcttgtggt
     4981 gggtatagat ggtggttatg ttcgcgatcg ggatgacaaa aagcgtaatt ttgaaattat
     5041 tgccgggaag tctttttccg ttggtgcgcc tgctgacacc cgccgcttcg gttttgttca
     5101 gaaggatgac tgtcatccgg aacgcaggct catgacccac ctttcagcgc agggaatgca
     5161 ggccaaccag cagatatttt ttctgtccga cggcgcggat aatctcaggg acctccagtt
     5221 cggtatgtac cctgagtcaa cgcatgtgct ggactggttt catatcacca tgaggctgac
     5281 ggtgctcatg caatatgccc ggggactgct ggtatcagat ccagaggcgg ggagtaaagt
     5341 tctggcactg ctggaaagca ttaaacgata tctctggcat ggcaatgttg tcgctgcgct
     5401 ggaacatatt gataattgcg ttatgtattg cgatgaccct gaactcagct atcctagcct
     5461 gaaatccctg caaaaacatc tggatgaaat gtatacctac atccggaata ataagatgat
     5521 gatcccaaac tacggcgaaa tgcgtcggta cggagagccg gtatcaaccg cgtttgtgga
     5581 atccacgatc aatgaagtga tcgccagacg aatggccaaa aagcagcaga tgcagtggag
     5641 cagaaaaggg gctcattatt tgttacagac ccggaccgcc gttttgaata acgaactgca
     5701 ggataaattt gtctgctggt atccgggttt tcaaagtgac gggaagggac cagcgatggc
     5761 agcatagctc acgctgcccc aatatttttt atgctctctc ctgtttgggg tcgtttgcgg
     5821 gaaggggcgg aatcctacgc taaggctttg gccagcgata ttctccggtg agattgatgt
     5881 gttcccaggg gataggagaa gtcgcttgat atctagtatg acgtctgtcg cacctgcttg
     5941 atcgcggccg cgatagctag atcgcgttgc tcctcttctc catccgcgtt ccaagctgcg
     6001 gaaaggcacc cataagcgta cgcctggtcg agcaggcgac gcggatcgac gtccagcgca
     6061 cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag gtcgtctctg
     6121 tcagccggat cgtagaacat attggcggcg ccaaagccca cttcaccgac cagaccgacg
     6181 ggatctatca ccagccagcc gcgactggag aacatgatgt tttcatgatg cagatcgcca
     6241 tgtagcccac gcagttccga ggcattgctc atcatttgat cggctataat cgccgcgtgg
     6301 acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa caaagctgca
     6361 aagcgatccc ggatcgggag aagggcagaa ggcaggggtt cctcagatgc ggcatacagc
     6421 ttcgccatta gttccgctgc aatttcggtc gcctggtagt cgccgtgctc ggcaacgatg
     6481 tgagagagca ttcgctcccc ggcatattcg agcaacatca gattgttctc acgaccgagc
     6541 aaccggactg ctcccctccc attgcgccat accagatagt cggccccgcg cagttcatca
     6601 gcaatgtctt ttataggttt caatcccttg acgattgcag gagtcccgtc tggcaatgaa
     6661 actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga aacgtgccaa
     6721 tgagcaggaa aaacaggcgg catgaacatc aaccccaagt cagagggtcc aatcgcagat
     6781 agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa tacattgaat aggacagcga
     6841 aggcgcgctc tgcttcatct ggcgctgccc agttctcttc ggcgttagca atcatgagtg
     6901 ccaaatcggc atagcgatct gctgttccga gccgcccaag gtcgatcaga cccgtgcatt
     6961 gaagagtttt agggtccacc atgaagttcg gcatgcaggg atcaccatgg caaacaacca
     7021 tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg ttcgacacga gccaaaagat
     7081 cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc gggattgacg gcattgcggg
     7141 acaccacatc aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac ggacattgat
     7201 caaccgatag gctgtgaaca gcgccaagtt gctgccccat tgacggccac gctttgagca
     7261 aatccgctcc agacagatca gccgccggta ctcccggaat tgccgttatc accaagcatg
     7321 caccctcctg ttcctcctgc cagttgatga cctcggggca agccacacct cgacctttga
     7381 gccaaatgag gcggtcacgc tctccagcga gctcaccgcg gcgggaagca ggtgcgattt
     7441 tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc accagattct ccgcctctga
     7501 caggcaacca gtcagaatgc gatttaccaa aaaaaatatt agttcgattc aatggaggtt
     7561 ccttcagttt tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc agttaacgaa
     7621 ttcttgcggt ttctttcagc gccgccaata ccgccagccc gtcgcgcaag gggcgcggct
     7681 cgtgtgtgcg gatgaagtca gctccacctg cggcggcggc aagctctgca gcgagtgtcg
     7741 cggccccgac atcccccgga ccacggcctg tgagcgcgcg cagaaaggat ttgcgcgaaa
     7801 cagacagaag caccggcaaa tcgaagcgca gccgcaattc atcgaaccgc gccagcaccg
     7861 agagcgaggt ttcgggagca gcccccagaa aaaaccccat gccgggatca aggacaaggc
     7921 ggttgcgttt gataccggca cccgtcagcg ccgcgatgcg cgcgtcaaag aacgccgcaa
     7981 tgtgatccat gatgtcgcca gcgggtgcct cgcgccgatc tgcctgcccg tcttgcaccg
     8041 aatgcataac gacgagtttg gcagatgatt tcgccaattg cggatagaac gcagcgtctg
     8101 gaaaaccgcg aatatcattg agataggcca caccacgcga caaggcatag gcttgcgtcg
     8161 cgggttgata actgtcgagc gagacgggaa tgccatctgc cttgagcgcg tccagcaccg
     8221 gcgcgatacg cgcgatttct gtgtcggacg aaacaggcgc ggcgtcggga ttgctggatg
     8281 ccggaccgag gtcgatcaca tctgccccct cggccatcag cttacgcgcc tgcgcaatgg
     8341 ctgcgtctgg cgccagatac cggcctccat cggagaaact gtccgaggtt atgttgacga
     8401 tgccgaaaat gatgagcgat ttattcatgg gggcttctat aataataata atcgagcatg
     8461 agtctcatac ggatgctcgg gtcgaaaggg aatccccagg cgagtaacct gtttgcggtg
     8521 ctccattagc tgcaggagca gaatagcata catctggaag caaagccagg aaagcggcct
     8581 atggagctgt gcggcagcgc tcagtaggca atttttcaaa atattgttaa gccttttctg
     8641 agcatggtat ttttcatggt attaccaatt agcaggaaaa taagccattg aatataaaag
     8701 ataaaaatgt cttgtttaca atagagtggg tatcggagac gaagaaggac aaggtacatg
     8761 ctgaccgata ccaagctgcg caatctcaag cccagggaca aactctacaa agtgaatgac
     8821 cgggaaggtc tctatgtggg ggtcgcctca gaaaacggaa tctatggtca ctcccgtttt
     8881 tgcaacaccg attttgacga caagttggct tgcttgaatc tatccggcgt ctgaatggga
     8941 ttttattccc gcgcctcgat gagttccgcg cctgatgaac ctccagaaaa tatacggctt
     9001 caatgagcct ttccgtttta caggttcctc aacaggccgg tgggccgtta gtatcatcaa
     9061 tatcagtatt cgcaaaacca gatgaatgat tgtttaaact ggtgtatttc tgcctttatg
     9121 cttcgtaagt ttgctgtcgc gccgtcagtg cccaggctat tctggccagc ttgtttgcca
     9181 gagcacaggt gacgacaaag ttgcttttcc gacacaacaa ctccctgacc cagtcggcca
     9241 acttgccaga ctggtgttcc agtttttgta tgaataccct ggcacactga accaacaaag
     9301 ttcggatctt tttgttgccc cgcttgctaa tccctaacaa tgtcgtccga cctcccgtgc
     9361 tgtactgtcg gggtaccagc cctgttgccg ccgcaaagtc acggctgctg gcgtactgct
     9421 tcccgtcgcc aatctcagtt gaaatagtac tggcagtcag cgttccaacg cagggaatac
     9481 tcagcaagcg ctgtccaacc tcatcttcgt ccaactttcg tttcaactga gattccagat
     9541 ctttaatctg ctcaacaaga tagtgataat gctgttgtaa tttcagcagt aactggctga
     9601 gataaagagg caaactactg tcctcaagaa gggtactcag tcgactaata acggcagcac
     9661 ctcgcggaac gctgatacca aattccagca gaaaagcatg catctgatta gttgttttca
     9721 ccttatcctg aaccagggat tcacggacac gatgcagagc tcgcattgcc tgctgagatt
     9781 cggttctggg ctgcacgaaa cgcatagatg gacgtgatgc tgcttcacag atagcttcag
     9841 catcaacgaa gtcatttttg ttgctttta
//
LOCUS       DAZFYC010000162         9853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFYC010000162 DAZFYC010000000
VERSION     DAZFYC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.163"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            356..970
                     /gene="evgA"
                     /locus_tag="ACHKW5_004591"
     CDS             356..970
                     /gene="evgA"
                     /locus_tag="ACHKW5_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO7513822.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            975..4568
                     /gene="evgS"
                     /locus_tag="ACHKW5_004592"
     CDS             975..4568
                     /gene="evgS"
                     /locus_tag="ACHKW5_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO7513823.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4624..5769)
                     /gene="yfdE"
                     /locus_tag="ACHKW5_004593"
     CDS             complement(4624..5769)
                     /gene="yfdE"
                     /locus_tag="ACHKW5_004593"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO7513824.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5843..6787)
                     /gene="yfdV"
                     /locus_tag="ACHKW5_004594"
     CDS             complement(5843..6787)
                     /gene="yfdV"
                     /locus_tag="ACHKW5_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO7513825.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6857..8551)
                     /gene="oxc"
                     /locus_tag="ACHKW5_004595"
     CDS             complement(6857..8551)
                     /gene="oxc"
                     /locus_tag="ACHKW5_004595"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO7513826.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8604..>9853)
                     /gene="frc"
                     /locus_tag="ACHKW5_004596"
     CDS             complement(8604..>9853)
                     /gene="frc"
                     /locus_tag="ACHKW5_004596"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO7513827.1"
                     /translation="STPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVT
                     RHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAID
                     HMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPL
                     VSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLD
                     KLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNW
                     ENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPC
                     APVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHT
                     AAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2922 a         2232 c         2036 g         2663 t
ORIGIN      
        1 tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag gcctaattta
       61 aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata caattcttac
      121 gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat catcggtata
      181 atccctgatt ttattgttga catttcattt atgccgacta tttatatggt atacttgtcg
      241 aattatctta aaggaagctc agattttctt atttttattg agaaaatgag atgacgcctt
      301 atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat aatctatgaa
      361 cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt tattgatcaa
      421 aaacgatatt gaaatcttag cggagttgac tgaaggggga agcgccgttc agcgggtgga
      481 aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta acggtatcca
      541 ggtgttagaa acgctgagga agcgccaata tagcggaatt attattattg tctccgctaa
      601 aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggat tcgtgagtaa
      661 aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg gctactgcta
      721 tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc aaaaactcga
      781 ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca aggataataa
      841 tgacattgct gaaaaaatgt tcatcagcaa caaaactgtc agcacttata aaagtcgcct
      901 gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcgc aacgtaacaa
      961 aatcggctaa tcacatgaag tttttaccct atttttttct tctctgttgt ggtctttggt
     1021 cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt agtaacaacc
     1081 gtgtcacact tgatccagta cgtctgagca acaaggaatt acgttggtta gcgagcaaaa
     1141 aaaatcttgt gattgcagta cacaagtccc aaacggctac cttgttgcat accgattcgc
     1201 agcaacaggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga gcgttaaata
     1261 tcaaattaac actccgggaa tacgccgatc atcaaaaagc aatggacgcg cttgcagaag
     1321 gtgaagtcga tatagtgtta tcacatttag ttacttcgcc gcctcttaat agtgacattg
     1381 ctgcaaccaa accattgata attacctttc cggcgctggt aaccaccctt cacgactcaa
     1441 tgcgaccgct tacctcacca aaaccagtaa atattgctcg ggtagcaaat taccccccag
     1501 acgaggtaat tcatcaatcg tttccaaaag caacaattat ctcttttaca aatttatatc
     1561 aggcattagc atccgtatca gccggacaga atgattactt tattggtagt aacatcatta
     1621 ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa tattataact
     1681 cgccgcgtca atataatttt ttcttgacca gaaaagaatc cgtcattctt aatgaagtac
     1741 tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtttca caaaattggc
     1801 ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa catgaaaaac
     1861 agtggattaa gcaacatccc gatttaaagg tgctggaaaa tccttactct cctccctatt
     1921 ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt aatattatta
     1981 ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc catgccggaa
     2041 cacagcttaa ccccggtgga tgggatataa tacctggcgc tatttatagt gaagatcgag
     2101 aaaataatgt ttcatttgca gaagctttca taacaacgcc atacgttttt gtcatgcaaa
     2161 aagcacctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt ccatattatt
     2221 atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata caggtcgata
     2281 atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg gtcgcgacac
     2341 agttaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat cattttctta
     2401 ttcctggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa ccggaactta
     2461 aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg cgcctgacgg
     2521 aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat agcgagcaat
     2581 tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta tggggattct
     2641 acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa aaccaaatat
     2701 cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta aactggcaag
     2761 gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat tactacaaaa
     2821 atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt tctaatgcgc
     2881 atgaagtcac agcagagacg aaagaaaatc gaacaatata cacacaggta tttgaaattg
     2941 ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat cttcctgcaa
     3001 gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt gatctaatta
     3061 atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa agtcagtttc
     3121 tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc ttcctggaac
     3181 ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca cttgcctacg
     3241 ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac aaaattgaat
     3301 cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc cagaacactt
     3361 gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc agcagtacat
     3421 ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc ttatcaaatt
     3481 tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg acctccctgg
     3541 ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct ggaagtggat
     3601 tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt gcaggtcgcc
     3661 agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa aatatgcagg
     3721 gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc acaatcccgg
     3781 tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc atcacactac
     3841 ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta ttactcaaac
     3901 gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg caagcgctac
     3961 acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg ccgaatatgg
     4021 atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc atctgggggc
     4081 ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc atgaacttat
     4141 gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag ttacaccagg
     4201 ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa aataatacgg
     4261 cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa acgcataaag
     4321 atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc catcagtgta
     4381 ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt aatattagcc
     4441 atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt cagttgctga
     4501 actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc tgtcagaaaa
     4561 atgactaaat agcggctccc acaatgttca aatgtgggag ctatttacca gcacatctta
     4621 catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg ttgccgctcc
     4681 cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg gcatcattat
     4741 tcccccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaatttaa tggcctcagc
     4801 cacacttaat aacggcgcga cgggtacacc aacttcatgt attctggcta accaaacttc
     4861 agctgcctgc gtttttaacg tccgctcaat atattgttta agaatagcct ggttttgtac
     4921 gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct ccagtgcctg
     4981 gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct tatcctgagt
     5041 attgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccagacgtt gtggtgactt
     5101 cccagttgcg atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg catcaaacat
     5161 cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg cactcactat
     5221 tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa cgcgcactgg
     5281 cggagcatca ggatatcctg tttccatcat tatcccgctc attgcctgaa tgatggtatc
     5341 gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg aagcatatat
     5401 gaggcgcggg ttgatttctt gaagcgtttc ccatgaaaac cccagttttt ccattgtacc
     5461 tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa atatactttt
     5521 atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa taaaactgta
     5581 atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac catgacccgg
     5641 cggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc cgaaaggtcc
     5701 attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt tgctttcatt
     5761 atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt cggcatttat
     5821 gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc cacaagggag
     5881 ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta cgggtgtaga
     5941 cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg cctgccagta
     6001 ccatcatctg cagatgttcg ctgttcaaat gacatgccat accaacgaga agcagtgcca
     6061 gtggcatcag aatcagcttc aggaaggtgt tataagcaat ttcggcactg aactcgaatt
     6121 tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta gccttcgcaa
     6181 tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccaac accaggatcg
     6241 ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat gcgctcagat
     6301 tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga ccaataggaa
     6361 tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt gatacggaat
     6421 caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt gaacccgcaa
     6481 ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag caaccaaacc
     6541 aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga cgagtgtccg
     6601 caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca ggaagagcgt
     6661 agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt tctcgtctgc
     6721 cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca ataaaaaatg
     6781 ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag ttggcaagag
     6841 gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg gtaatatggc
     6901 cactttcagt tcctgctgca gggtcgatga ccacattaat aatggtcggt ttgcgcgact
     6961 gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta tagccaacgc
     7021 cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc gttggtgatg
     7081 gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca ttattaaaaa
     7141 taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc ccactgaaac
     7201 caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg ctagcaccga
     7261 tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga cgacgacgtg
     7321 gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct tcattaacta
     7381 aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca ttaaagtaat
     7441 ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt tgctgcttgt
     7501 ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt ttcagttctg
     7561 ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca atggggcggt
     7621 tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct gccgcccatc
     7681 cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg acaacgtcag
     7741 catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta tcttcaagga
     7801 tccctttcgc catagacatt ggcaggaatg gaatctgagt actttcaata aattcacgaa
     7861 gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat ggccgttcag
     7921 cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat aatgctggcg
     7981 acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc gtcgcggcca
     8041 ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca gagacccgga
     8101 tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat gctgctttgg
     8161 catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc tgtaggtcga
     8221 cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt accgttgcgt
     8281 tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg cagatccccg
     8341 gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc tcatgacgaa
     8401 aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc acaggaatac
     8461 ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg atgatatgca
     8521 taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt ttataaattg
     8581 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
     8641 tcgtcgctat aacccagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
     8701 gctttaatat ccggcgtaaa ggcagagaat ttcattggac aaccaacggt cagatatttt
     8761 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
     8821 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
     8881 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
     8941 atatgtggct gtcgtgcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
     9001 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
     9061 ttaggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
     9121 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
     9181 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
     9241 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
     9301 agcaaagcag caagtaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
     9361 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
     9421 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
     9481 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
     9541 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
     9601 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
     9661 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
     9721 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
     9781 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
     9841 agtggagttg aca
//
LOCUS       DAZFYC010000163         9645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFYC010000163 DAZFYC010000000
VERSION     DAZFYC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.164"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1231
                     /locus_tag="ACHKW5_004597"
     CDS             <1..1231
                     /locus_tag="ACHKW5_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7513828.1"
                     /translation="KLIPQMNYLMVVVALFFLNAIIFLFMLMKYFTNKQILPTLILSL
                     AFLSGLIYLVETIVVIHKPISDSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKD
                     NILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYT
                     KILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
                     IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDF
                     CVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTEL
                     NSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKA
                     LREAKSEGGNKVIIHHI"
     gene            1412..2902
                     /gene="dgcJ"
                     /locus_tag="ACHKW5_004598"
     CDS             1412..2902
                     /gene="dgcJ"
                     /locus_tag="ACHKW5_004598"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO7513829.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEKRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2945..3448
                     /gene="yeaK"
                     /locus_tag="ACHKW5_004599"
     CDS             2945..3448
                     /gene="yeaK"
                     /locus_tag="ACHKW5_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO7513830.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3449..3553)
                     /gene="yoaI"
                     /locus_tag="ACHKW5_004600"
     CDS             complement(3449..3553)
                     /gene="yoaI"
                     /locus_tag="ACHKW5_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO7513831.1"
                     /translation="MNDQMFVETLIISSSFFAIAVVLVLSVLLIERTG"
     gene            3723..4169
                     /gene="yeaL"
                     /locus_tag="ACHKW5_004601"
     CDS             3723..4169
                     /gene="yeaL"
                     /locus_tag="ACHKW5_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO7513832.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4126..4947)
                     /gene="nimR"
                     /locus_tag="ACHKW5_004602"
     CDS             complement(4126..4947)
                     /gene="nimR"
                     /locus_tag="ACHKW5_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO7513833.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVIMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWACFFAMSERNIARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5044..6225
                     /gene="nimT"
                     /locus_tag="ACHKW5_004603"
     CDS             5044..6225
                     /gene="nimT"
                     /locus_tag="ACHKW5_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO7513834.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6280..6627
                     /gene="yeaO"
                     /locus_tag="ACHKW5_004604"
     CDS             6280..6627
                     /gene="yeaO"
                     /locus_tag="ACHKW5_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO7513835.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6649..6903)
                     /gene="yoaF"
                     /locus_tag="ACHKW5_004605"
     CDS             complement(6649..6903)
                     /gene="yoaF"
                     /locus_tag="ACHKW5_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO7513836.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRYHPAGEK"
     gene            7086..8111
                     /gene="dgcP"
                     /locus_tag="ACHKW5_004606"
     CDS             7086..8111
                     /gene="dgcP"
                     /locus_tag="ACHKW5_004606"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO7513837.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLDDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8144..8242
                     /gene="yoaK"
                     /locus_tag="ACHKW5_004607"
     CDS             8144..8242
                     /gene="yoaK"
                     /locus_tag="ACHKW5_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO7513838.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8245..8319
                     /gene="yoaJ"
                     /locus_tag="ACHKW5_004608"
     CDS             8245..8319
                     /gene="yoaJ"
                     /locus_tag="ACHKW5_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO7513839.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8378..8626)
                     /gene="yeaQ"
                     /locus_tag="ACHKW5_004609"
     CDS             complement(8378..8626)
                     /gene="yeaQ"
                     /locus_tag="ACHKW5_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7513840.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8854..>9645)
                     /locus_tag="ACHKW5_004610"
     CDS             complement(8854..>9645)
                     /locus_tag="ACHKW5_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO7513841.1"
                     /translation="SRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLD
                     AGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSR
                     IANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSI
                     LDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCG
                     YTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         2355 a         2333 c         2266 g         2691 t
ORIGIN      
        1 aaaactcatc ccacagatga attatctgat ggtcgttgtg gctttgtttt tccttaacgc
       61 catcattttt cttttcatgt taatgaaata tttcactaac aaacaaattc taccaacact
      121 cattttaagt cttgcatttt taagtggcct tatctattta gttgaaacta ttgttgttat
      181 ccataaacct atcagcgaca gtacactgat ccagacaaag tcgaatgatg tttctatttt
      241 ctatattttc cgccaactca gttttatttg tttaacctcg ctggcgctct tttgttatgg
      301 taaagacaac atccttgaca acaataagaa aaaaactggc attctgttgc tggcgctgat
      361 tcctttttta gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc
      421 tttgtatgtc gtcgattact gtccggacaa ccatactgcg acctggggaa tcaactatac
      481 aaaaatattg gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt
      541 agccagcgaa ctatggccgt taatagcatt attatgtctg gcatcgctat gctgcaactt
      601 acttctactg actctggatg agtataatta caccatctgg tatatcagtc gcgggattga
      661 agtttccagt aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggaactgca
      721 actctccagc aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt
      781 tttcaacagc gtagagtcat tattgtcgcg acctgttgtt aaggactttt gtgtcatgct
      841 ggttgatatt aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa
      901 agtgctggtt tcaattgtcg atattatcca gcaaagcatc cgccccgatg atattttagc
      961 gcgactggag ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa
     1021 aatcattgcg gaacgtatgc gtaaaaatgt cgaactcctg accggcttta gtaacagata
     1081 tgatgttcct gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg
     1141 taatatctcg cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg
     1201 gggcaacaaa gtgattattc atcatattta agtgcaaaaa tattcagagc catgcttttt
     1261 gcgtggcttt tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc
     1321 agagatgagt ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct
     1381 cgtttcacta accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca
     1441 ttttatcgcc gcaagtgtca ttgtgctgac atcttccttc cttatttttg aacttgtcgc
     1501 cagcgacaga gcaatgagcg cctatctgcg ctatattgtg cagaaagcag actcctcctt
     1561 tctttatgat aagtatcaga atcagagtat tgccgcgcat gtgatgcgcg ctctcgctgc
     1621 tgagcagtcg gaagtgtcgc cagaaaagcg gcgcgccatc tgcgaggctt ttgagtctgc
     1681 caataatacc catggcttaa acctgactgc ccataaatac ccgggcttac gcggcacact
     1741 acaaaccgca tccactgact gcgacacaat tgtggaagct gcagcgctat tacccgcttt
     1801 tgatcaggca gtggaaggca accgccacca ggatgattac ggttcaggtc ttgggatggc
     1861 cgaagaaaaa tttcactatt atctcgatct caatgaccgc tatgtctatt tttatgagcc
     1921 ggttaatgtt gaatactttg cgatgaataa ctggtccttc ctgcagtcag gaagtattgg
     1981 catcgatcgc aaagatattg aaaaggtatt taccgggcgt accgtattgt cgagcattta
     2041 ccaggatcag cgtactaaac agaacgtgat gagtttgctg acgccggtat atgtcgcagg
     2101 gcagctaaaa gggattgtgc tgctggatat taacaaaaac aatctgcgga atatctttta
     2161 tactcatgac cgccctctcc tctggcgttt tctcaatgtc acgctaaccg ataccgattc
     2221 ggggcgcgac attatcatca accagagcga agataatctg ttccagtatg tcagttacgt
     2281 ccatgactta ccgggcggca ttcgtgtctc gttatccatt gatattcttt actttatcac
     2341 gtcttcgtgg aaaagcgttc tgttctggat tttgacggcg ttaattttgc tgaatatggt
     2401 ccggatgcac ttccgtttat accaaaatgt gtcgcgagaa aatattagtg atgcaatgac
     2461 tggactgtat aatcgcaaaa ttttaacccc tgaactggaa cagcggttgc agaaactggt
     2521 gcaatccggt tcttcggtga tgtttattgc tattgacatg gacaagttaa agcaaataaa
     2581 tgacaccctc ggtcatcagg agggggattt agcgattacg ttattagctc aggcgattaa
     2641 acagtcgatt cgtaaaagtg attatgccat ccgactcggt ggcgatgaat tctgcatcat
     2701 tcttgtcgat tcgacgccgc aaattgcagc acaactgcct gaacgtatcg aaaaacgtct
     2761 gcaacatatc gcgccgcaga aagagatcgg cttctcttcc ggtatttatg cgatgaaaga
     2821 aaacgatacg ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca
     2881 gaacaaaaac agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagca
     2941 tgaaatgact gaaatggcta aaggaagcgt gacgcatcaa cggttaatcg ctttattatc
     3001 acaagaaggt gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt
     3061 gtcagaaatt cgtggcaccg ctttaggcca gggagctaag gcactggtat gtaaggtcaa
     3121 aggcaatggc gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct
     3181 gagccaactc gccagccata taggcggatt acgcgcttcg ttggccagcc cggcggaagt
     3241 cgatgaactc acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact
     3301 caaactggtt gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg
     3361 catgctggat aaatccgtta ttttgaaaac cgcggattat ttgcgcattg cgcaaccaga
     3421 actggtcaat ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca
     3481 aaaccagcac aaccgcaatg gcaaaaaacg atgacgagat aatcagtgtc tcgacgaaca
     3541 tttgatcgtt catagtatgc ccccgggaga tagctgtttt cacgttattg ttagcgtgca
     3601 ccaatggcag tttgatgaca gttcgcctga ttttttatgc aaaaaacgta aagattttct
     3661 acttccttcc tgcagcaagc gtaaagtaag caggcttatt attttttggc aaggaaacca
     3721 cgatgtttga tgtcactttg ctgatcctgc tcgggttagc tgcgctgggc tttatcagtc
     3781 ataacaccac tgtcgccgtt tcaattctgg tgttgatcat tgtccgcgtc acaccgttaa
     3841 gcaccttttt cccgtggatt gaaaaacagg ggctaagtat cgggataatc attctgacga
     3901 ttggcgttat ggcccccatc gccagcggga cgctaccgcc ctcaaccctg attcactcgt
     3961 ttctgaactg gaaatcgctg gtggcaattg cagtaggcgt tattgtctcc tggctgggtg
     4021 ggcgcggcgt gacgttaatg ggcagccagc cgcaactggt tgctgggctg ctggtgggca
     4081 ctgttttggg cgtggcgctg tttcgcggcg taccggttgg cccgcttatt gccgcgggtc
     4141 tggtttcgct gattgtgggg aaacagtagt taatcccgca atatatcggc ctggcgtttg
     4201 ccccaggcct ttcttaaaca tagtaataaa ggccgttgtt gaatcatacc ccagcgtatg
     4261 agcaactttc tgtacagtat cgcctttcac taatccctga agcgccataa tcagctgtaa
     4321 ctgctgtcgc cactggcgaa aactcaaccc cgtctctttt actatcagtc gggcaatatt
     4381 gcgttcactc atcgcaaaaa agcaagccca ttgccccaat gacccccact cgacgggctc
     4441 tttcgccatc atctccacca tcgcgcggat tttaggatga gaagaaacgg gtaggtgcag
     4501 ctgttgttgt ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat
     4561 cggctcagca cgctgagtcg tcgtcctgtt tgccagagtt aaaatcaatt cccggcataa
     4621 gggcgatatt ttcaatgtgc aacaggttgt cggcattatc actgcgctgg gttcgataaa
     4681 cagaaaacag agttcagcgt tagctgtcac ttgattgcta tgttccaccc ctcccggtat
     4741 ccaaaccgca tattgcggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc
     4801 accatgtagc gccagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc
     4861 atccgttccg gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata
     4921 gccattgaga ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta
     4981 gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag
     5041 catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt
     5101 ctgatgattg ccacaacatt acgcgtcacc tttaccggcg cagcgccgtt actggatacg
     5161 attcgttccg cttactcgct gacgacagcg caaaccggct tattaaccac cctgccatta
     5221 ttggcctttg cgctaatctc acctttggct gccccggtgg cgcgacgttt tggtatggaa
     5281 cgtagcctgt ttgccgcgtt acttttgatc tgtgcaggta tcgcaattcg ttctctccct
     5341 tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat
     5401 gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc
     5461 gcatattccc tgacaatggg agctgcagcg gcactgggat cggctatggt cgtgccgctg
     5521 gctttgaacg gttttggctg gcaaggcgcg ttgttcatgc tgatgtgttt tcctctgctg
     5581 gctctttttt tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacctcg
     5641 cgcgccttac atactcgggg tatctggcgt tcgccgcttg cctggcaggt cacattgttt
     5701 cttgggatca actcactggt ctattacgtg attattggct ggcttccggc gatcctcatc
     5761 agtcgcggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca
     5821 gcagctcccg gtttgctgat ccctcttttc ttacatcatg tgaaagatca gcgtggtatt
     5881 gcagcgttcg ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg
     5941 cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg
     6001 ttgacgttca ttggtcttcg ggcaagttct gcgcatcagg cggcggcact ctcggggatg
     6061 gcacaatccg tcgggtattt gctggcagcc tgtgggccgc cgctgatggg taaaatacac
     6121 gatgctaacg gtgactggtc cgtaccactg atgggtgttg ccatactttc actactgatg
     6181 gcgattttcg gactttgcgc cgggagagac aaagaaattc gctaatatcc ggtgctatag
     6241 tgacgtgaca aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc
     6301 gtttatgatc cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggccg
     6361 cgcggtatca aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca
     6421 acggaactgc gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa
     6481 tatcttgcag aactggcgca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa
     6541 aaacagcccc tgaccctgct ctacgcagca aaaaacacca cgcagaacca tgcgctggtg
     6601 ctggccgact ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc
     6661 agccggatgg taccgtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt
     6721 gcaattgttg ctgacccctt gcgctgtctg cactggaatg agcgtcccgc ccttctgctg
     6781 gcaatagacc gacgccggat ttgccatacc aatctgcggc ggtttaggtg cttctggctg
     6841 agaaggggtt gaacaaccag ccaggaccag caagcaaggc agaacaaaac tgataatttt
     6901 catttattga tctcacatat ttatccagac ttagagtatc gcggtatcgt tttgttttgc
     6961 agcactattt ttattacatt cactcaaatc atattacgcc ttgtttcatc tttgttgatg
     7021 atgttttatc atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt
     7081 gtttcgtgtc agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac
     7141 tggaaagtct ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa
     7201 cctacctgac caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca
     7261 gccagaaaat gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac
     7321 gcgccattga tgaaaattgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta
     7381 ttgcggcacg caatcttggc atcaccacat ttctgagcac gccaattcac ttaccggatg
     7441 gatcattcta tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg
     7501 cggaacaggt tttacagtta ttcgctggac tgattgcaca atatattcaa aaagaggcgc
     7561 tggttgaaca gctgcgcgaa gccaatgctg cgctgattgc gcaatcgtat accgactcgt
     7621 taaccgggct accgaatcgg cgggcgattt ttgaaaatct gacgacgctg ttttctctcg
     7681 cccggcatct taaccataag ataatgatcg cgtttatcga tctggataac ttcaaattaa
     7741 tcaatgaccg ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc
     7801 ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg
     7861 ttgtttcact aaacaacgag aatgcggata tttcgtcgct gcgagaacgt attcaacagc
     7921 aaatacgtgg agaatatcac ttagatgatg ttgatttgta ttatcccggt gccagtcttg
     7981 gcatagtaga agtcgatcct gaaacaaccg atgcagacag tgccctgcat gctgccgata
     8041 ttgcgatgta tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg catccagcgc
     8101 tacattcctg aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat
     8161 ttttccggtt gtaatcttta ttacagcggt cgtattttta gcatggtttt ttattggcgg
     8221 ctatgctgcc ccgggagcat aaagatgaaa aaaacaacga ttattatgat gggtgtggcg
     8281 attattgtcg ttctcggcac tgagctggga tggtggtaac gtcacctcta aaaaatagca
     8341 aaggctgcct gtgtgcagcc tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga
     8401 taaatagcac gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat
     8461 cgactttacc aaagccaaac agtgtgctga tccatccgcc gactacggca ccgactatcc
     8521 ccagcagaat agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca
     8581 gaataccggc aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta
     8641 tctgttttgc attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca
     8701 agatttgtga cgagcatcac ggaggatttt tttgcggatg gcacagaaat tgcgccatca
     8761 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ttgacatcct
     8821 ccacgccctg aatgacgagg atccctgcta cgttcaggct gtcgcctgaa tcatttcggt
     8881 gggttcctgc ttcaacgagc ggcctgactg caccatctct ccacaggcaa gaacggcgtg
     8941 ccccgccgct aaaatgttac gagcgccgtt tacatcggcg ttcgctgtat atccacatac
     9001 ctggcatctg aattgacttt gtgacaggcg attttctttc gctgtatgac cacagcacgc
     9061 gcaacgctgg cttgtgtacg ctggcggaac agcaagcacc tgaccgccac gccagagctg
     9121 cttgtactca agctggcggc gcatttcata ccagccctga tccagtatcg aacggtttaa
     9181 acctgatttt gcccggacat tgcgccccgg ctggctgacc gtacccgctg ctgactttga
     9241 catgttgctg accttcaaat cctcaatgac tatcattgcg tggtttttgc tgacggtcgt
     9301 tgtgactttg tgaaggtagt ccctgcggat atttgcgata cgggaatgca gtcgctgtat
     9361 tttgcgtttc tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg
     9421 aagtctcgcc agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc
     9481 atctgacagc gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg
     9541 gtgaaccgga gttgatactt cactttctgt ctgaatactg atgtaccact taccgcagga
     9601 ctggctgaca gtgacatttt tcacaacacc cgtgacctga cggct
//
LOCUS       DAZFYC010000164         9530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFYC010000164 DAZFYC010000000
VERSION     DAZFYC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.165"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(69..1544)
                     /locus_tag="ACHKW5_004611"
     CDS             complement(69..1544)
                     /locus_tag="ACHKW5_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7513842.1"
                     /translation="MLWCKESLIYLLFSVLVTWCCGYTVKNTLGNVLILFLMLLLGRQ
                     NVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQTNLNEAGEFITGIPVLYFIV
                     SLIIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPKRMVTNVMTGIAETREEMMRY
                     ESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSVYGYPLPTTPWLNSAPGIFIN
                     GYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKAGYETWWISNQGSLGRHDTLI
                     SVIAANADKKYFLKEGSSSSRRIDDEDMLQYVREAVNSSSPKVIFIHMLGSHPNPCDR
                     LFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTTEHGDDFAMLYFSDHGQSVSS
                     DSTPVHHAPGLRSGYDVPLMMMACDITEHHLNHVHISARRFPEIFQWITGIQTKNLPL
                     SFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAESEK"
     gene            complement(1802..2257)
                     /locus_tag="ACHKW5_004612"
     CDS             complement(1802..2257)
                     /locus_tag="ACHKW5_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFO7513843.1"
                     /translation="MRDKSEVLAGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLSGLCLLFIYSFFKRKRVVGKLLLAIHLLYIPHYA
                     ITFVYCLYVVGAECMTTGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(2345..2968)
                     /locus_tag="ACHKW5_004613"
     CDS             complement(2345..2968)
                     /locus_tag="ACHKW5_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7513844.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKVPTARLKLALESRLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            3117..3620
                     /locus_tag="ACHKW5_004614"
                     /pseudo
     CDS             3117..3620
                     /locus_tag="ACHKW5_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242778.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            3975..8054
                     /gene="epeA"
                     /locus_tag="ACHKW5_004615"
     CDS             3975..8054
                     /gene="epeA"
                     /locus_tag="ACHKW5_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EpeA"
                     /protein_id="HFO7513845.1"
                     /translation="MNKIYSLKYSSLTGGLIAVSELSKKVKGKTGRKLMTASVALSVS
                     LSALPVEASTVSAEIPYQTFRDFAENKGVFTPGATGIEIKDKNGNLTGVLNTPMLDFS
                     SLSRDGHTTLVHPGYGVSAKHGGLQSLKTATFGYNTEYKIVNNNHASYDFTAPRYGKL
                     VTEVIPVNTGDGKYNDSKKYTAFYRAGVGDQYIKDKKGNHTLLNRYDPQNAYLTGGTV
                     GTPYYGRVSNQTVLISSPGNTFNQTHGPLASYGQLGDSGSPLYAWDNIQNKWVLAGVT
                     LYNEGVNGSRNWWLELPNSFINETIKSDSKEIVVKNKKGDITWSFDKTLGTGVINLEK
                     KDHIINGHKNNNPDHGDNIVFSGEEGVINITNDIAQGAGFIQFDNNYIVSTQNNSTWT
                     GSGIIVNDGSEVYWGINGVKDDNLHKLGSGKLIINGNGINTGGLKVGDGTVLLSQHKD
                     AEGRVQAFSSVNISSGRPVVVLSDDKQINPDNISWGYRGGTLDLNGNNITFTRIQASD
                     YGAIITNNNDKKSITNLNISTLKDTDINVSVSDVHVLGGKGKPGDLFYDRGTGRYLIL
                     KKERYSPFFQDVNNTSVWQYVGKNRNEAIAVVKNEKIKNSSKPYLFHGQLNGNMDINI
                     QSGMGVNTFAIDGSVNIPNGTLSNKLGSLIFQGHPVIHAGVGVSAEQNDWETRYFTLN
                     TLNLDGSDFHLSRNAVMTGNIHATNSSKVTLGSSSVYVDRNDGTGESVNATSGTASDI
                     NEKDLSKFTGDITADNSNIIINNKFEGGVSAVNKSAIDIHSQHAVINRWSDISDNSKL
                     TLKKNATLTVNTGLVNKGTIEIGENAELNLQGYPIADKFIPSIHDLGNVKMTASNATL
                     TAGNYAMFSGEITADDATAVRVNLGSETSTLSEFNPNPELTDLMFDKYNTSWTGKISA
                     LKGDASMVNTVWRMTGDSGLNTLKTSKSLTVFSSDNKFSTLTVNDLTTSDSTFVLRSD
                     STGSDKVVVKNKLEGKNNNLLVDYVANDGKHNSLNLELVSAPKGTAADVFNSQTQNVG
                     FSDVTPVIEQKDSGEKTTWTLKGFNAVANQQSAEKAENFMSAGYKNFLAEVNNLNKRM
                     GDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHKLDGLDLFTGFTVTHT
                     DSSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYS
                     THSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLI
                     GRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMS
                     VGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            8358..8519
                     /locus_tag="ACHKW5_004616"
     CDS             8358..8519
                     /locus_tag="ACHKW5_004616"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513846.1"
                     /translation="MKNIKTHTGLLIHKEQTRRVRLHETPTAWCHTHRECYSKTTGRR
                     AAALTVSAG"
     gene            8559..8924
                     /locus_tag="ACHKW5_004617"
     CDS             8559..8924
                     /locus_tag="ACHKW5_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513847.1"
                     /translation="MNDGRQTPPPPEAWKIRFLANGTPGTWFYTDDAGVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTKS"
     gene            8934..9125
                     /locus_tag="ACHKW5_004618"
     CDS             8934..9125
                     /locus_tag="ACHKW5_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513848.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACQDAFTAL
                     KKENVGEFALRGVCVPAGE"
     gene            9156..9479
                     /locus_tag="ACHKW5_004619"
     CDS             9156..9479
                     /locus_tag="ACHKW5_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO7513849.1"
                     /translation="MTSPDLIQADLHRMSWSQLAKAAEEATVHHDYARALILWRHAYH
                     AATLTINKNLATAKINFCAKRILMRNQMSKIIRHTDTDEILFRLSKHHEPPRKYWRLN
                     SLRKR"
BASE COUNT         2971 a         2109 c         2024 g         2426 t
ORIGIN      
        1 tcagcctgaa ataaacgctc aatgcctcat acacatgagg cattgagctc ggtcataaat
       61 aatctttatt atttttctga ttcagcattt ccggtcagta tcggctcttc tggcaaagac
      121 attaccggaa cgtcacgttc accattgaat acagaaacac tttcagcgaa atcattctgt
      181 aacaaagaaa aacttaacgg cagatttttg gtttgtatcc ctgtgatcca ttgaaaaatc
      241 tcgggaaaac gacgtgcact gatatgcaca tgattgaggt ggtgctcagt aatatcacag
      301 gccatcatca tgagaggtac atcgtatccc gaacgcaaac ctggcgcatg atgcacaggg
      361 gtgctgtctg aactgacgga ttgcccatga tcagaaaaat acagcatggc aaaatcatcg
      421 ccatgctctg tcgtcatgtt ataaattgcc tgaagaaacc gatccagctt aagcaatgtt
      481 gataaataac aggatacttt atttccgaat aattccctga acggctgtgc atcattaaaa
      541 agtctgtcac agggattagg atgggacccc agcatatgaa tgaaaatcac ttttggagaa
      601 gacgaattaa cagcttccct gacatactga agcatatctt catcatcaat gcgacgggaa
      661 gaagagctcc cttccttaag aaaatatttc ttatccgcat tggcggcgat gacagaaatc
      721 agcgtatcat gtcgccctaa agagccctga tttgatatcc accacgtctc atatccggct
      781 tttctggcaa gcgtaacaat attattaccc ggattacctg tttcagtgta atcaagagtc
      841 agtgttctgc tgagtgaacc tatcgtattc ggagctgcgg agaaataccc attaataaat
      901 atacccggag cagaatttaa ccatggtgtt gtcggcaaag gatacccata aacagaaaga
      961 taatcccgtc tgacactttc accaattacg ataattatgg ttttatattt cttctccaca
     1021 ctcacaatat cccatgagtc tttctgattg tttttcagcg attcatatcg catcatttct
     1081 tctctggttt cagctatccc tgtcattaca ttcgtaacca tacgttttgg ccatgcattt
     1141 actgacaata cgataaagaa caacagaaag ataaatcccc ggtggctttt atcacgctta
     1201 cgccagaacc agaatgtaaa agcagcaatg atcagtgaaa caataaaata aagaacaggt
     1261 atgcctgtga taaattcacc tgcctcattt agatttgtct gcattacaga cgcaataaag
     1321 ctgttgttta tttttccgta aatcatccca accggagcat agaatgcact taacaaaaga
     1381 aagatacaga cacacgttgc tgaaataaca ttctgccgcc ccaataacaa catcaggaac
     1441 aatataagca cattccccag ggtgtttttc actgtatatc cgcaacacca tgtaacaagc
     1501 acagaaaaca acagataaat cagtgactct ttacaccaca acataaaata aaactgtttt
     1561 gctctcaaaa gcatattttg atttttcttt aatacaagac acgccagtca ttttcctgta
     1621 caaacacaga cgatcatttc aacgcatgga tatttatatg aaacagttca caaaaatacc
     1681 ctaccctgtc cagcaagatt tacatattat caggttctgt gtttacagca actgctcccc
     1741 gaaccagatt attcgcataa cccgatgaag gcctcataac cataaaacac tcagaataca
     1801 tttagttaac aaaaatatgt cttgccctcc cggaaaggca aacatacgaa agagaaagca
     1861 tggctataat aatatcaatc aaaatatgct caatctttcc ggttgtcata cactcagcac
     1921 cgacaacata aagacaataa acaaaagtga tagcatagtg cggtatataa aggagatgaa
     1981 tagccagaag taattttcct accactcttt ttcgtttaaa gaaggaataa atgaatagca
     2041 aacataaccc tgacagaaaa agaaaggtag caatggagat tataaaatac tccgtcacct
     2101 gtgaattctc atccagagat gaatacagcg aataatatgt ggttgtgaga ttaagtcttg
     2161 ttatcaatat ataaacaact gataatagta taatataaag aatcagacca aatctgtttt
     2221 tctgcgctcc ggctaatact tctgatttat ctcgcataaa atatcctgtc tgtaatctgt
     2281 cccggtaaac aatggtaaat ttcagaatca gtgcgaatac ggtaacaaag attcctcctg
     2341 tgctttaaag tgtgtaagca gaaaccagtc tggtggttcc ggtcagggag tctgatacct
     2401 caacttcccg ggtgactata accatacctg actgacgcag tctctgcaca tcagctgcaa
     2461 ttcgctgagt tcccagccag aacaggccat ccagtgccgt taccagcaac ctgctttcca
     2521 gcgccagttt cagccgtgcc gtcgggactt taacctgccc ggcgatatca gtaaaacaac
     2581 gcgtcgtcat gccatagcag tccatccgcc gtattcgcgt cgcaagactg tagctgaaac
     2641 aatccggcac gccggataag tcattctgca gactgtatac acatccccgc tgccgcccgt
     2701 tgaacagggc ttttttctga ctgagcagcc attcccgacg cagagtgtca atcatctgcg
     2761 gtgtccgggt taacagcaga cgaaacggca gctcaaaact ggtcacataa tcaacattca
     2821 gtaatgctgt ccgcagtcgc tccctggcac ctgccccggt ctcccggcat tcacgcatga
     2881 catcagccca ctgacgcatc agtcgtggag actcattcct ggccctgaac tgatatttct
     2941 caatatgata atcaatacgt tcctgcatat cgccaccaga tatctgttgt ttacccagac
     3001 acagtgatta gcctacagga ataaatatcc tccgtgaagc caggtgcctc agaagataaa
     3061 aacatgtact tttttcaacg tgtaatatta acaccaccac cacaggaaaa tataaaatga
     3121 taaaatatat attcgcactc acacttctgc tttccgggtc tgtctgtgcc gatcaaattc
     3181 agggactcgt tattcgtgtg ctggacggag atactctgga gatattacag gcccggcacc
     3241 ccgtgaggat tcgtcttgtg aatattgatg cgccggaaaa gaaacagcct tttggacgct
     3301 ggtcaatgaa tcaacttaaa agccttgtgg caaatacgcc cgtaaccgtg acatacacac
     3361 agacagaccg ctacgggcgt attctgggac gggtatatac cgaagacggc atggaagtta
     3421 accgctggct ggtacagcat ggtgcagcct aggtatatcc tgatttcaac acagattaca
     3481 ccctaccggc ataccagaga gaggccagaa caatgaagcg gggtctgtgg gcagataaaa
     3541 aacctgttcc tccatggctg tggcgaaaag aaaataaaaa tgaccacaca gaattacaaa
     3601 caaacaacat cttaccgtaa tatgtatttt ttctgctgca ttattttccc tgtttgcagc
     3661 cagcagaaaa caactttccg aaatgaaata aacgcgaata ataaacctac ttaatgaatc
     3721 tattacgaaa gtgaatgctt tctcttatgg aaaacgcagc aattacaaag ggggggaaca
     3781 gataagactg agttctgaat cacaaaaggc accaataaaa tataaacttt tcttgatatt
     3841 ttctctctat gacttcttct ttttttatgg acatattatt ttgttgattt taaaatatca
     3901 aaatgagtga atgtatcaca tcactcataa agtggatatt atcacgaaac aatctattaa
     3961 atggcagata caacatgaat aaaatttact cactaaaata tagtagcctt actggtgggc
     4021 ttatagctgt atcagagtta agtaaaaaag ttaaaggaaa aacaggcaga aaattaatga
     4081 ctgcttccgt tgctttatct gtgagtcttt cagccttacc cgtagaggca tcaacggtca
     4141 gcgctgaaat accatatcag actttccgtg attttgctga aaataaaggc gtatttacac
     4201 ctggtgcaac cggaattgaa ataaaagaca aaaatggaaa cctgactggc gttctcaata
     4261 cccccatgct tgacttctcc agtttgtctc gagatggtca tacgacgctg gttcatcctg
     4321 gctatggtgt ttctgcaaaa catgggggat tgcagagtct taagacagcc acctttggtt
     4381 ataataccga atataaaata gttaataaca atcatgcatc atatgatttc acagctccac
     4441 gatatggtaa attagtaaca gaagttattc cagttaacac tggagatggg aaatataacg
     4501 acagtaaaaa atatacggca ttttatagag ccggggttgg tgaccagtat ataaaggata
     4561 agaaagggaa tcatacactc ctgaacagat atgaccccca aaatgcttat ctgacaggtg
     4621 gtactgtcgg cactccctat tatggacgtg tcagcaatca aactgttctc attagttctc
     4681 cagggaatac atttaatcaa acccatggac ctttggcaag ttatggccag ttaggagata
     4741 gtggctctcc tctttatgca tgggataaca tacagaacaa atgggttctt gctggagtta
     4801 cgttatataa tgaaggcgtt aatggctctc gcaactggtg gctggaactc cctaattctt
     4861 ttataaatga gacaataaaa agtgactcaa aagaaattgt tgtaaaaaac aagaaaggag
     4921 atatcacctg gagttttgat aaaacgctgg gcactggtgt tattaatctt gaaaaaaaag
     4981 accatataat caatggccat aaaaacaaca acccagacca cggagataat atcgttttct
     5041 ctggggagga aggcgttata aacataacga atgatattgc tcagggggct ggatttattc
     5101 agtttgataa caattatatc gtatccacac aaaataacag cacatggaca ggttctggca
     5161 tcattgttaa tgatggaagt gaagtatatt ggggaattaa tggtgtaaaa gatgataatc
     5221 ttcacaaact aggaagcggt aagctgatta ttaatggtaa tggcataaat acagggggat
     5281 taaaagtagg tgatggcact gtgttactta gtcaacacaa agatgctgag gggagagttc
     5341 aggcatttag ttctgtaaat atttccagtg gacgtcctgt agttgtgctg tcagatgata
     5401 agcagattaa tcctgataat atatcatggg gatacagagg tggtactctt gacttaaatg
     5461 ggaataatat tacatttacc cggatacagg cctccgatta tggtgccatc attactaaca
     5521 ataatgataa gaaatcaatc accaacctga atattagcac cctaaaagac actgatatca
     5581 atgtttccgt tagtgatgtt catgtgcttg gcggaaaagg aaaacctggt gatttatttt
     5641 atgacagagg gacaggcaga taccttattc tgaaaaaaga aaggtattcg ccattttttc
     5701 aggatgtaaa taataccagc gtctggcaat atgtgggaaa aaaccgtaat gaagcaatag
     5761 cagtagttaa gaatgaaaaa atcaaaaaca gtagcaaacc ttatcttttc catgggcaat
     5821 taaatggcaa catggacatt aatattcaga gcggcatggg cgttaatacc tttgcgatag
     5881 atggctcagt caatatacca aatggaactt taagtaataa gttaggcagc cttatattcc
     5941 agggacaccc tgtcattcat gccggggtcg gtgtttctgc agaacagaat gattgggaga
     6001 caaggtattt taccctcaac accctcaatc tggatggttc tgatttccat ttatcccgta
     6061 atgcagttat gactggaaat attcatgcca ccaactcaag taaggtaacg ctgggtagtt
     6121 caagtgttta tgttgatcgt aatgacggta caggagagtc agttaacgca acatctggca
     6181 cagcgtcaga tataaatgag aaggatttaa gcaaatttac tggagatatt acagcagaca
     6241 actcaaacat tatcataaac aataaattcg aaggtggtgt ttccgctgta aataaaagcg
     6301 ccattgatat tcatagccaa cacgccgtaa ttaacagatg gagtgatata tcagacaaca
     6361 gtaaactaac tttaaagaaa aatgcaaccc ttacagttaa cactggattg gttaataaag
     6421 gcactattga aatcggagaa aatgcggagt taaatcttca gggatatccg attgctgata
     6481 aattcattcc gtcaatacat gacctgggga atgtgaaaat gacggcgtca aatgccaccc
     6541 tgacggcagg taattatgca atgttcagcg gtgagatcac tgctgatgac gccactgctg
     6601 tcagagttaa cctgggctca gagacatcaa cactctctga atttaatcct aatccggaac
     6661 tgaccgacct gatgtttgat aaatataata catcatggac cggcaaaatc tctgccctga
     6721 aaggcgatgc atcaatggtt aataccgtat ggcgtatgac gggagattcc gggcttaata
     6781 ccctcaaaac cagcaaatcc ctgacggtat tcagcagtga taataaattc tccacgctga
     6841 cagttaatga cctgacaacc agtgacagca cctttgtgct gcgttctgac tctacaggct
     6901 ctgataaggt ggtagtaaaa aacaaacttg agggtaaaaa caataatctt cttgttgatt
     6961 atgttgcaaa tgatggaaaa cataattcgc taaatcttga actggtcagt gcaccgaaag
     7021 gaactgctgc cgatgtcttt aattcacaga cacaaaatgt gggcttcagt gatgtaacac
     7081 cggtgattga acagaaagat agcggagaga aaaccacatg gaccctgaag ggattcaatg
     7141 ctgtagctaa tcagcagtca gctgaaaaag cggaaaattt catgtcagca ggctataaaa
     7201 attttcttgc tgaagtcaac aacctgaaca aacgtatggg tgacctgcgt gacatcaacg
     7261 gcgaagccgg tgcatgggca cgcatcatga gcggtaccgg ctctgccagt ggtggtttca
     7321 gtgacaacta cacgcacgtt caggtcgggg tcgacaaaaa acataagctg gacggactgg
     7381 atttgtttac cggtttcact gtcacacaca ctgacagcag tgcctccgcc gatgttttca
     7441 gtggtaaaac gaagtctgtg ggggctggcc tgtatgcttc cgccatgttt gattccggtg
     7501 cctatatcga cctgattggc aagtatgttc accatgataa tgagtacact gcaacctttg
     7561 ccggactcgg aacccgtgat tacagcacgc attcatggta tgccggtgca gaagcgggct
     7621 accgctatca tgtcactgag gatgcctgga ttgagccaca ggctgagctg gtttacggtt
     7681 ctgtatccgg taaacagttt gcatggaagg accagggaat gcatctgtcc atgaaggaca
     7741 aggactacaa tccgctgatt ggccgaacgg gtgtggatgt gggtaaatcc ttctctggta
     7801 aggactggaa agtgacagcc cgtgccggtc tgggctacca gttcgacctg ctggctaatg
     7861 gcgaaaccgt attgcgggat gcatctggtg aaaaacgcat caaaggtgaa aaggacagcc
     7921 gtatgctgat gtccgttggc ctgaatgcag aaatcaggga taacgtccgc tttggactgg
     7981 agtttgagaa atccgccttt ggtaagtaca acgttgataa tgcagtcaac gctaacttcc
     8041 gttactcgtt ctgatactcc gtattcaaag ggcctgcggg ccctttatgc tcagtgcctg
     8101 ccgccctgat atgtctgaga gacatttccg ggatgtcagt cgactggttt acttaactgc
     8161 gatgactaaa gctggcagta atgccggctt ttttacggcc acatacgtct tcctctcatc
     8221 cggaactgtg atacaaatcc tgcatacagg attgaccgcc ggttcctgca aatggcacat
     8281 tttcagtaag atagcgaagg ttacattccc ccgtcatgtg tggcacaagc tgtcaccggg
     8341 aaagaaacgg aaagcacatg aaaaacatca aaactcacac cggactcctt atccacaaag
     8401 aacaaacgcg gcgcgttcgt cttcatgaaa cacccactgc ctggtgccac acgcaccggg
     8461 agtgctacag caaaacgacc ggtcgtcgcg cggcagccct gacagtctca gcaggctgat
     8521 tctgagcagc attcagccca tagaatgaga taccccctat gaatgatggc agacagacgc
     8581 ctccgccccc cgaggcatgg aaaatccggt ttctggcgaa cggtacaccc ggcacatggt
     8641 tttacaccga tgacgcaggc gtcacaaaag agtggctgcg ggcacattgt gacgatgtgg
     8701 aagtgatccc cctgtgccgt atgcatgacg gtcccgtttt ccgggaacac catattaatg
     8761 aactcagcgc cgcactggcc agaatgctcc atatgcacca catactgctg gaacgaaccc
     8821 ggtacagaga aaatatccat gatgcagaat gtattcgtga aattcacgaa gcccccctgc
     8881 aggctatcgg catcctggaa aaatacaaac gcacaaaatc ataaggcagt tctatgtgga
     8941 tactgatttt ctggctctca tcccccttta atgtctcctc cggcgatgcc cggcaaccca
     9001 cactggctgt tcacagtcag gagttccgta ctgaaaaagc ctgtcaggat gccttcaccg
     9061 cgctgaaaaa agaaaacgtg ggggaatttg ctctccgtgg cgtctgcgtc cccgccggag
     9121 aataaacaag acatgatgcg caaccggagt ccgctatgac atccccggac cttatacaag
     9181 ctgatctgca ccgaatgtcc tggtctcagc tggcaaaagc cgcggaagag gcgacagtac
     9241 atcatgatta tgcccgggcg ttaatattgt ggcggcatgc atatcatgcc gccacgctga
     9301 ctattaataa aaacctagca accgctaaaa tcaacttctg tgctaagcgc attctgatgc
     9361 gtaatcaaat gagtaaaata atcagacata cagacacaga cgaaatactc ttccgtctga
     9421 gtaaacacca tgaaccgccc cggaaatact ggagactaaa ctccctgaga aagaggtaaa
     9481 caggatgact aaaaatactc gtttttcccc cgaggtccgt caacgggcag
//
LOCUS       DAZFYC010000165         9272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFYC010000165 DAZFYC010000000
VERSION     DAZFYC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.166"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..402
                     /gene="yfdR"
                     /locus_tag="ACHKW5_004620"
     CDS             <1..402
                     /gene="yfdR"
                     /locus_tag="ACHKW5_004620"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7513850.1"
                     /translation="SVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPD
                     YKRMEEKIDAVIREKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPAT
                     EMFNVIPLAPGHAYGMFMERFNDLSELRKCA"
     gene            393..755
                     /locus_tag="ACHKW5_004621"
     CDS             393..755
                     /locus_tag="ACHKW5_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513851.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            755..1060
                     /gene="yfdT"
                     /locus_tag="ACHKW5_004622"
     CDS             755..1060
                     /gene="yfdT"
                     /locus_tag="ACHKW5_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFO7513852.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            976..1155
                     /locus_tag="ACHKW5_004623"
                     /pseudo
     CDS             976..1155
                     /locus_tag="ACHKW5_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1287..2450
                     /gene="intD"
                     /locus_tag="ACHKW5_004624"
     CDS             1287..2450
                     /gene="intD"
                     /locus_tag="ACHKW5_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO7513853.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(2466..2542)
                     /locus_tag="ACHKW5_004625"
     tRNA            complement(2466..2542)
                     /locus_tag="ACHKW5_004625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2506..2508),aa:Arg,seq:tct)
     gene            2785..3417
                     /gene="fimZ"
                     /locus_tag="ACHKW5_004626"
     CDS             2785..3417
                     /gene="fimZ"
                     /locus_tag="ACHKW5_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO7513854.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(3420..3935)
                     /gene="sfmF"
                     /locus_tag="ACHKW5_004627"
     CDS             complement(3420..3935)
                     /gene="sfmF"
                     /locus_tag="ACHKW5_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO7513855.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(3946..4953)
                     /gene="fimH"
                     /locus_tag="ACHKW5_004628"
     CDS             complement(3946..4953)
                     /gene="fimH"
                     /locus_tag="ACHKW5_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO7513856.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(4966..7574)
                     /gene="sfmD"
                     /locus_tag="ACHKW5_004629"
                     /pseudo
     CDS             complement(4966..7574)
                     /gene="sfmD"
                     /locus_tag="ACHKW5_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(7605..8297)
                     /gene="fimC"
                     /locus_tag="ACHKW5_004630"
     CDS             complement(7605..8297)
                     /gene="fimC"
                     /locus_tag="ACHKW5_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO7513857.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPTDAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(8517..9059)
                     /gene="fimA"
                     /locus_tag="ACHKW5_004631"
     CDS             complement(8517..9059)
                     /gene="fimA"
                     /locus_tag="ACHKW5_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7513858.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         2700 a         2132 c         2079 g         2361 t
ORIGIN      
        1 agcgtcgccc aacatgcggt tctttgcagc cagctggtgc cgcaggaatt tgcttttgaa
       61 gcgttaatgc atgatgcaac agaagcgtat tgccaggaca ttcccgcacc actgaaacgc
      121 cttcttcctg actataaacg gatggaagaa aaaatagacg ccgtaatccg tgagaaatac
      181 gggttacccc cagttatgag tacacccgtg aaatatgccg atctcatcat gctggcaacc
      241 gaacgccgcg atctcgggct tgatgatggc tctttctggc ctgtactgga aggcatcccg
      301 gcaacagaaa tgttcaacgt gattccactg gcaccgggtc atgcctacgg gatgtttatg
      361 gaacgtttta acgatttatc ggagttacgc aaatgcgcat gaatgttttc gaaatggaag
      421 ggtttcttcg cgggaaatgt gtaccgcgag atctgaaagt gaacgaaaca aatgctgagt
      481 acctgttacg taaattcgac gcgcttgaag ctaaatgtgc ggcactggaa aacaaaataa
      541 taccagtgtc agctgaactg ccaccagcaa atgaaagtgt tctgttattt gatgctaatg
      601 gagaaggctg gctgattggc tggcgttctc tctggtacac ctggggacaa aaagaaaccg
      661 gagaatggca gtggacattt caggtcgggg accttgaaaa cgtcaatatc actcactggg
      721 cagtaatgcc aaaagcaccg gaggctggag cataatgacc acatttacca ataaagaact
      781 gattaaagaa atcaaagaac gaatcagcag cctagaggtt cgagacgata ttgagcgccg
      841 tgcttatgaa atcgcactcg tatctctgga agtagagcca gatgaacgcg aagcctatga
      901 attattcatg gaaaagcgtt tcggtgactt agtagatcgt cggagagcaa aaaacggcga
      961 taacgaatac atggcatggg atatgactct cggttggatc gtctggcagc aacgagctgg
     1021 tatccatttt tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca
     1081 ctcgatgagg cctgtcattt tctcaaaata tccagaccga ctgccattaa ctggatacgc
     1141 acaggccgcc tacaggcaac acgtaaagat ccaaccaagc caaaatctcc ttacctcaca
     1201 acacggcaag cttgcattgc ggcgcttcag tctccgctgc atactgtcca ggtgagcgcg
     1261 ggtgatggca taacagagga aagaaaatgt cactcttccg cagaaatgaa atatggtatg
     1321 cctcgtattc gctcccgggc gggaaacgaa ttaaggaatc tcttggcaca aaggacaagc
     1381 ggcaagctca ggagttgcac gacaagcgaa aagcagaact ctggcgagta gaaaagctag
     1441 gggatttacc tgatgtcact tttgaagagg cctgcctaag atggcttgag gaaaaagctg
     1501 ataaaaaatc tctcgattca gataaaagcc ggattgagtt ctggcttgaa cattttgagg
     1561 gtataaggct taaagatatc tcggaggcaa agatttactc tgctgtaagc agaatgcata
     1621 acagaaagac gaaagaaata tggaaacaga aagttcaggc cgccatcagg aaaggtaaag
     1681 aaccgcctgt ttatgaacca aagccagtat caactcagac aaaggcaaag catcttgcca
     1741 tgataaaggc cattctccgt gctgcagaac gcgactggaa gtggctggaa aaagcgcctg
     1801 tcatcaagat accagcggtc agaaacaagc gagtcagatg gctggaaaag gaggaagcaa
     1861 aacgccttat tgatgagtgc cccgaaccac tgaaatctgt cgtcaagttt gcgctggcaa
     1921 ctggtctgag aaagtcgaac atcataaatc tggaatggca acaaatcgac atgcagcgac
     1981 gagttgcctg ggtgaatcca gaagagagca aatcaaaccg cgccattggt gtggcgctga
     2041 acgataccgc ctgtaaagtg ttgcgtgatc aaataggcaa gcatcacaaa tgggtgtttg
     2101 tacataccaa ggcggctaag cgagcagatg gaacatcaac gcctgcggtc aggaagatgc
     2161 gcatcgacag caagacatca tggctatcag cttgtcgtcg tgcaggaatt gaagatttcc
     2221 gtttccatga cctcagacac acctgggcaa gctggctgat ccagtcaggc gtcccattat
     2281 ctgtgcttca ggaaatgggc ggatgggagt ccatagaaat ggttcgtagg tatgctcacc
     2341 ttgcgcctaa tcatttgaca gagcatgcaa ggaaaataga cgacattttt ggtgataatg
     2401 tcccaaatat gtcccactgt ggaattatgg aggatataaa gaaggcgtaa ctgattgaat
     2461 tgtaatggcg cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc
     2521 tatccaactg agctaagggc gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac
     2581 ggtcaaccct tgctgagtca atggcttttg atctggttgc tgaacaagtg aacgaccgcg
     2641 tctgattttc tgatttattt cgctatagcg gcaaacaaac acacaccgct gcgcgtctga
     2701 atcaagaaaa cccgtatttt catgtatcaa agtacaattt accgccctaa cggaaaattg
     2761 tccgctccta tgagactggt aactatgaaa ccaacgtcgg tgatcattat ggatactcat
     2821 cctatcatca gaatgtctat tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc
     2881 ctgaaaacgg atgattaccg cataaccatc gattatctcc gaacccgtcc tgttgattta
     2941 atcattatgg atatagactt gcccggaaca gacggtttta ccttcctgaa aaggatcaaa
     3001 caaatccaga gcacagtgaa agtgttattt ttatcatcga aatcagaatg cttttatgct
     3061 ggcagagcga tacaagctgg tgctaacggt tttgtcagta aatgcaatga tcagaatgat
     3121 atttttcatg ccgttcagat gatcctctcc ggatacacgt tttttcccag cgaaacgctt
     3181 aactatataa aaagcaataa atgtagtacg aatagttcaa cggtcactgt gctatctaat
     3241 cgtgaagtga ccatattacg ttatctggtt agcggattat ctaataaaga aattgccgat
     3301 aagttattac ttagcaataa aacagttagt gcgcataaat ctaatattta tggcaagcta
     3361 ggtttgcatt caattgtaga gcttatcgac tacgccaaat tatacgaatt aatataatat
     3421 taattataat tgatcataaa tatcgcatcc gctttcgcca cacctggccg aacaccgctg
     3481 gctaacgctc gataattggc aaaaaagttt agtgtgacat tgccatttgc atctacttcc
     3541 tcagtcgggc tcgccgctcc cagtgcgagc cgggagcgat cgctattacg taattcgatg
     3601 gcgatggttt gcgccattgc gggatcatcc agagccagca ggttggtatc ggatgccggc
     3661 gttcccgtaa ataaaatcgc aactgaaccc ggaggacatc cctccagccg caggctaaaa
     3721 gggacgagtg ccgtggtatc gccagcgttc agtaattgtg tcgtaggcca tctgcctaaa
     3781 tctaccgtct tatcaatatc cgcagtgttt acggtacagg agaaatcaac aacgttaccg
     3841 tgcaaattga tattaatcgt tcccaaaggg tcagctgccc atccactgga aatccacagt
     3901 aacccgcaga aacagctaaa gagtgctctt ctcattctcc gtacctcatt gataatcgac
     3961 gcgtaaatac cccagcgcgc taaacggccc ttcggtcggt ttttgaccgg taatactgat
     4021 aggccaggcg cgaagtgtga cattggctgc cgcagctgca tccagacgga aaggaataac
     4081 gctattgaga tcgttaggcg tgatcggcgt atcgttctga tcggcgacaa taaaacctaa
     4141 atcctgattg tccgacacca tcgcctgacc agaaacggca ctggcttcca gacgcattgt
     4201 taaataagcc tgcgcagcaa cattcgtaca tttgaccgca atgctcttgg tttgcggcat
     4261 gacaccagca ggtcgattac ccggccctgc cgcactaaat aacgatgcgc cgatatcacc
     4321 aaaatcaaat tcaacaatct gcccggcatt taattcgcag ttttgcggta cttcaacccg
     4381 gccaccaaaa ctaatggtat aaacaggtgt aaccaacgga tcgtaaggcg ttgatgtcac
     4441 atacacggta aacatcacct gtctggggat ctccaccatg ttaataaagg gacgaataac
     4501 ctttaaacga aatattaaac gagaatccat caccccgaat ggaaggtttt gcgaaacgtt
     4561 aggatctgtt cccatcagaa tataattttg cggggggaac tgaatatcca tcacactatc
     4621 aaccagactc atcgcaccta atagataatc atttaattgc atatatttat aattatcgat
     4681 agtttcctga acaataaagt tggtgacata actacggtat gtataattca ccagcgtacc
     4741 gggtggacaa attgctgaga cacctaccca gccggatttt tccggtaaaa caacaatctg
     4801 ccctggctgg ttattggtgc tattaaagat atttgagaga tcatagtgtt catcttctgc
     4861 tacgccagta ctatttgcac aaacagtagc ccaggatatg tttgccagac acaaacatgc
     4921 catcgccaat aataatctgc agattatctt cattgataat ttcctttatg agccaggatg
     4981 tgtgcaaact gccgaaataa cagtgacggc ttgctgtaag ctttgcttcg gcaatacata
     5041 gtgcgcaatg cagcgtgagt tcgcgccgtc tccccactga accagtaatt caccagacaa
     5101 tggcgcacca cttaaataaa cttgcccgtc atcacccaca tactggttat tccgatactg
     5161 ttttcccgta ccagtgatcc aaacggcacc ggttttccgc cctgggtaac ggtaatgagc
     5221 gcccgcacgc ctatgcgggt atcaaaattg gcacgaacca acgcgccttg cgtcggcaca
     5281 acgctgctaa tatttttttc aacatcgatg gaattcccca tcgtattggt atcaagcgcg
     5341 atacggttat accgataaac cgtggcatac ggcatcaccg catagccgcg ccagtcggtt
     5401 aaaatgccag tttgattttc aatgcgtaca ccgcctgcgc caggcgcttt aatcaaaaca
     5461 ttggtatccc ctaaaggctg gctcagcgtt atgccatttt catgcccgac cacaccgcca
     5521 gacagctgcc agttaacgtc atgttgatcg cggtcgtagt tatacccgac ccccagcgta
     5581 gcgtaagcgg cctgccagtt tgccgtggcg ctgcccgtat acccattatt cgaggtatca
     5641 ccctggctta cgtgataact caggttgtgg ccttccagta aggtcccacc tacacctgcc
     5701 agccagctat tttgcccgtt gctgttacgg ttggcgttaa aggaggcata agcgcggtcg
     5761 agcgcatttt cccgggtgta gcgacgtttg gtcagaacat tgaaaggcac tgaaacatta
     5821 agtccgacaa tacgttcgtt atcggggatc cctacagatt cattccacga aaatgagagt
     5881 gaataactga tgccaaccca gctgctggta taccccacct gataccaggt atctgaatcc
     5941 gaagtattcc agtatttttg atgggtacca gagatataaa gcgagccaaa gtcattaagt
     6001 gattgtgaaa tatttaactg caaacggtct ttacggctaa agcgtaaatt gtggtaattc
     6061 acgattatcg gttcatcccg atgctcaccg tcgtaatcgt aatcatattc gtacccctcc
     6121 attcgacgat acgcaacatc atcaagggta taaaaacctt gtgtcgaata gcggtaaccc
     6181 attaactgaa aattggtgcc gaaggtgttc atcgatttcg catagaggaa gcgaatggaa
     6241 tccccctcat gacgactgtc gtcggctaac tgactgcgcg catgggtcac atccagcgac
     6301 actgcccccc agtttccgag attgcgcccc agtcctaata aaaaggcggt gtaattggca
     6361 gataattgcg tcccgccgta ggcagtaaat tcctgtggta aaccgccgag tgccgtgccc
     6421 tgaaaaaaga aaggcgatga ttgctgacta ttaccgctac gaaaatcgcc cgccgtcagg
     6481 tcaaatttga aacgcccttc gcgttgtaaa attggcactg ttgaatacgg gatagtgtaa
     6541 ttctgctgat taccatcgcg ctcgtcgatg gtgacgtcca gatcgccatt tgaagatgtc
     6601 gggtgcaaat ctgtaatttc aaaagcgccg ggggaaacat agctttgata gataataaaa
     6661 ccattttgcc gaatcgttag ctgggccgcc gtacgggcaa tcccacgtac cgttggggca
     6721 aacccttgct ggctatcagg atacatatta tcggaagaat aaagccgtac accacgaaat
     6781 ccaacaccat cgaaaatatc acttcctgta ttgccgtctc ccattaccag ttcacttttc
     6841 agcggaataa tggcgcgctg tacccaggtg ccaatattat tccactgttc tgaatgatat
     6901 ccatttccgc gaaaatagtt ccaggaacca ttgttgcgta aacgccacgg gccaatatta
     6961 atcccgctga gctcactaaa aaaatagcta tcgttaccat tacctctgtt accggtgaaa
     7021 ttataattca ggagtaaagc aggaatacct tcatcccact cttcaggcgg aatgtaaccg
     7081 tgagcgctac tcaacaacgt tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
     7141 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
     7201 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
     7261 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
     7321 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg atacactccc
     7381 gcaggttgat gattgcctct ctcaaagcgc gataaatcag caacggatgt gccattctct
     7441 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggagag taaaatggca
     7501 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
     7561 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
     7621 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
     7681 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
     7741 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
     7801 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
     7861 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
     7921 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
     7981 agagactcac gatctgctgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
     8041 cttttgggtt cactaacaaa cagtggcggt gtaatgatga atgacttttc ttttacaccg
     8101 ctgctgtttt caatccacga attgaccaga aaacgctcat tggtatggct atttctaatc
     8161 cataccgcag tctgtttagc atcagtggga taaataatac gcgtggcacc taatgcgatc
     8221 cctccggcag catgggcgct ggccgaaatg attaaataaa atataatgag catcaataac
     8281 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa aataagaaat
     8341 ataaagaaat gattgctttg gtttcttaca acacatcatt cccttttcat ttaataaagc
     8401 ggcatgatac aaagtgaaca tgatttcgat ttacattgcc accataccgc ttcagacaac
     8461 cggtacatta ctttgttata agacgtgata tataattcaa aacaacgtgg ttttgattat
     8521 tcgtatctca taataaaagt cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg
     8581 gtgcctttat aacgtgcaga aaaatgaaga acgttggttc cggggatcaa gttctggttg
     8641 gttgagaagc tattcccatc aggtttgaga attgcggatg tattatcaag tatttcaata
     8701 ccgacacccg ttgctgttgt tgtattggta ctggatgcaa tggccaataa attatcattg
     8761 attgcatcag cctgaccaga aaatgcaacg gcagcattag cggcaacaac aggatcgcag
     8821 tcgttcaact gaatggtgaa tggaattaat gcagaggtat taccaacagc attgaaaata
     8881 tcggtacgat attgaccgag tgtgacaacc tggtctgccg agtcagtatt cactgaacag
     8941 gcagcattca ccagttcgcc ttcaaagtga attgtaccgc catctacaac ggcagcataa
     9001 ctacccgtag cggcgaatag tgcggcagcc agcgcagacg aaataaatct taatttcata
     9061 tgtattcctt caatctcatt tatcgactcc acatccgtat ataaccgatt actttattta
     9121 agacactgat agtagtaaaa ttccttttta tcctctaaga atgtcttaat tgaaaatatg
     9181 cactctattc taaaaaatag agagccccgt tagatgaata cttccgcgca aaatatattc
     9241 aacaaaaata tagacctgaa gcggtaaatt ac
//
LOCUS       DAZFYC010000166         9092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFYC010000166 DAZFYC010000000
VERSION     DAZFYC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.167"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            27..1349
                     /locus_tag="ACHKW5_004632"
     CDS             27..1349
                     /locus_tag="ACHKW5_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO7513859.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1377..1688
                     /locus_tag="ACHKW5_004633"
     CDS             1377..1688
                     /locus_tag="ACHKW5_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO7513860.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1744..3417
                     /locus_tag="ACHKW5_004634"
     CDS             1744..3417
                     /locus_tag="ACHKW5_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO7513861.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3442..4881
                     /locus_tag="ACHKW5_004635"
     CDS             3442..4881
                     /locus_tag="ACHKW5_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7513862.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHTRG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4938..5156)
                     /gene="marB"
                     /locus_tag="ACHKW5_004636"
     CDS             complement(4938..5156)
                     /gene="marB"
                     /locus_tag="ACHKW5_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO7513863.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5188..5571)
                     /gene="marA"
                     /locus_tag="ACHKW5_004637"
     CDS             complement(5188..5571)
                     /gene="marA"
                     /locus_tag="ACHKW5_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO7513864.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5591..6025)
                     /gene="marR"
                     /locus_tag="ACHKW5_004638"
     CDS             complement(5591..6025)
                     /gene="marR"
                     /locus_tag="ACHKW5_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO7513865.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6237..6902
                     /gene="marC"
                     /locus_tag="ACHKW5_004639"
     CDS             6237..6902
                     /gene="marC"
                     /locus_tag="ACHKW5_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO7513866.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6927..8117)
                     /gene="ydeA"
                     /locus_tag="ACHKW5_004640"
     CDS             complement(6927..8117)
                     /gene="ydeA"
                     /locus_tag="ACHKW5_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO7513867.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8267..9078)
                     /gene="yneK"
                     /locus_tag="ACHKW5_004641"
                     /pseudo
     CDS             complement(8267..9078)
                     /gene="yneK"
                     /locus_tag="ACHKW5_004641"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2504 a         1938 c         2164 g         2486 t
ORIGIN      
        1 atatccaaaa caacaggtat aaaaaaatgg gattaatggc ctcattcgaa cgtggaatgg
       61 aacgttttct tgttccagtt gctatcaagt taaactcaca aaaacatgtt gcagcggtga
      121 gagatggatt cgtttttacg tttccaatta tcatggcaag ttcattaatt atattaatta
      181 actttgccat attatcgcca gacggtttta ttgccggatt actgcatctg aatagcattt
      241 tccccaacct tgaaaaagca caagctattt ttactccggt aatgaatggt tctgtaaata
      301 tcatgtcaat tatgattgct ttcctggtcg ccaggaatat ggcgattagc tatgagcaag
      361 atgatctttt atgcggatta acggcaatag gagcattttt tattgtttat acgccatatc
      421 agatgataga tgggcaagca ttcctgacga ccaaatatct cggcgcgcag gggttgtttg
      481 ttgctgttat cgttgcattg atcaccagtg aaatattttg tcgcttagct cgaaacccaa
      541 aaatcaccat aacgatgccg gcagctgtac cgcctgcggt agcgcgttca tttaaagttt
      601 tattgccaat attttttgtc atggtgttct tttccgcact taattattgc ctgacactga
      661 tatccccggc aggactaaac gatcttattt acacattaat ccagacgccg ctcaaacata
      721 tgggaacgaa tatctttgcg gtaattatcc tgggggctgt gggtaatttc ctgtgggtgc
      781 tggggatcca cggacctaat accacatcgg caattcgaga aaccgttttt tctgaggcta
      841 atctggagaa tctctcctgg gccgctcaac atggcactac ctggggcgcg ccatatccga
      901 ttacctggac ttctattaat gatgcattcg ccaactgcgg cggttcaggt atgacgttgg
      961 ggttattgtt ggctattttt atcgcttcta agcgtgcgga ataccgtgat ctggcaaaaa
     1021 tgtcatttat ccccggtatt ttcaatatca atgaaccgat aatgtttggc cttcctattg
     1081 tacttaaccc catcatgatg gtgccgttta ttatggttcc cattgttaac tgtgccattg
     1141 gttacttctt tgtttcgatg gaaattattc caccggttgc ttatgccgtg ccttggacta
     1201 cgcccggacc tttaattgct ttcctcggaa ccggggggaa ctggctggct ttactggttg
     1261 gttttttatg tttaggtgtg gcgacaatga tctatttacc ttttgttatt gccgccaaca
     1321 aagtcaataa catgacaact aacggataat tccatttacc caggaaggag attgtgatgt
     1381 ttgcagatga agaacttgtt atggagttac tggtcaacgc ggggcaggca cgttctgacg
     1441 cgatggaagc catacgttgt gccgggcaaa aagactggca gggcgcaact cagctaatgg
     1501 ccagttctga atctgcctgt cttcaggcac ataagattca gaccgcatta attagtcagg
     1561 atgaaggctg tggcaaaata aaagttaatt taattctgat tcatgcacag gatcatttga
     1621 tgaatgcaat attatgccag gatttagcca gggaaattat ttcattaaga aaagaactcc
     1681 atgcctgatt ttttcctcca gtgcgatagc tggaggaaat tattatactc ttaaggataa
     1741 gtcatgaata taaagacgct taacgtcagc cttttgtctt tttctattat tacagcattg
     1801 tttccattga acgcgatggc aacaaaatta accatagagc agcgccttga attgcttgaa
     1861 aatgaattgt cgcaaaataa acaagagctg aaagcaacac agaatgaact gggagtatat
     1921 aaatcccgac tttcgacctt acaaaaaagc atcacggaaa acaaatataa atcagcctcg
     1981 cttgctgaaa tatcagcgac atctcccgtc gctgataaca tcaaaaatga aaacggtgaa
     2041 cagaactcgt ttaccgcagc gcatactata aatggatcgc agcaagttgc cgttattgaa
     2101 agtaaaggcg acaaaaccac tatcgaaagc gtgaccctga aagatatcag taaatatata
     2161 aaggatgata ttgggttcag ctatcagggg tacttccgct caggctgggg tactggtaat
     2221 cacggctcac cacaaactta tgcagcgggt tctctgggac gttttggtaa tgagatgagc
     2281 ggttggtttg acctgacctt aaatcagcgt gtttataatc aggacggtaa aacggcaaat
     2341 gcggtcgtta cctatgatgg caacgtgggt gagcagtata acgatgcctg gtttggtgac
     2401 agtgccaatg aaaatatcat gcagttcagc gatatttacc tgacaacgcg aggtttttta
     2461 cccttcgcac cagaggcaga cttctgggtc ggcaaacata aactcccgca atatgagatc
     2521 cagatgctgg actggaaaac cttaaccacg gatgtcgccg cgggtgtggg gattgaaaac
     2581 tgggcacttg gtgtagggct gtttgatatg tccttaagcc gtgatgatgt cgatgtttac
     2641 tctcgtgatt tttcgcgtac cagccagatg aataccaatt ctgtggatgt tcgttatcgc
     2701 aatatcccgt tatgggatga tgcgacatta tcattaatgg ctaaatattc cgcacctaat
     2761 aaaacggatc aacaacaaga taacgaaaat gacaacagtt attttgaaat gaaagatagc
     2821 tggatgctga cttctgtttt acggcaaaac ctgcaacgcg atacgtttaa tgaatttacg
     2881 ttacaggttg ccaataattc ctatgccagc agttttgcca gtttctcaga tgccagtaat
     2941 acgatggcgc atggtcgcta ttactatggt gatcatacca atgggatcgc ctggcgttta
     3001 atctctcagg gcgagatgta tcttactgac aatattatta tggctaacgc gcttgtctat
     3061 tctcacggcg aagatgttta tagttatgaa agtggtgctc atagtgattt tgacagtatt
     3121 cgcactgtaa taagaccggc ctggatctgg aatacatgga atcagacggg gcttgaatta
     3181 ggctggttta agcaacagaa caaaactcag cagggtgtaa cactaaatga atcggcttat
     3241 aaaacgacac tctggcatgc attgaaagtg ggtgaaagca ttttaggttc acgaccagaa
     3301 attcgcttct atggaacgta tatcaatatt ctggataacg aattatcgaa ttttaagttt
     3361 aatgagaaca gcaaagacga gtttatggcc ggcatccagg cggaagtctg gtggtaatct
     3421 attttagtta aggagttgtg tatgtcagga tttaaaaaag gttttttatg gggcggcgcg
     3481 gtagccgcgc atcagttgga aggtggctgg aatgaaggag gaaaaggcat cagcatcgct
     3541 gatgtgatga ctgctggcgc tcacggggtg ccgcgtgaag tgacagaagg cgttatcgac
     3601 gggcttaatt atcccaatca tgaagcaatt gatttttatc atcgctataa aacagatatt
     3661 cagttatttg ccgagatggg attcaaatgc tttcgaactt ccattgcctg gacacgaatc
     3721 tttccgcaag gtgacgaaca ggagccgaat gaagagggtt tacaatttta tgatgatctg
     3781 ttcgatgaat gccttaagca gggaatggaa cctgtggtga cgctttcaca ttttgagatg
     3841 ccttatcatc tggtgacaaa atatggtggc tggcgaaacc gtaaactgat cgactttttt
     3901 atccgcttcg catcaacggt cttcacgcgc tataaagaaa aagtaaagta ctggatgacg
     3961 tttaacgaaa tcaataatca ggtgaatttc agcgaaagcc tgtgtccatt tactaattcc
     4021 ggtatcttgt attcgccaga ggaagatatc aatgagcgcg aacaaataat gtaccaggcg
     4081 gtacattacg agttagttgc cagtgccctg gcggtacaga ctggaaaatc gatcaatcct
     4141 gaatttaaca ttggctgtat gatcgccatg tgtcccatct atcctctgac gtgtgcaccc
     4201 aacgatatga tgatggccac gaaagcaatg catcgtcgtt actggtttac tgatgttcat
     4261 actcgtggat attatccgca acatatgctg aattactttg ccaggaaagg attcaacctc
     4321 gatatcacac cagaagataa cgcgattctt gccagtggtt gtgtcgactt tatcggcttt
     4381 agctactaca tgtcttttac gacacaattt tcgcccgata acccgcaact ggattatgtt
     4441 gaaccacgag atttggtcag caacccttat atcgatacat ccgaatgggg atggcaaatt
     4501 gatccggcag ggctacgtta ttcactcaac tggttctggg atcatttcca gttgccgctg
     4561 tttattgtcg aaaatggatt tggtgcggtt gaccagagac aagctgacgg cacggtgaac
     4621 gatcactatc gcattgatta ctttgcttcc catattcggg aaatgaaaaa agccgtagtt
     4681 gaagatggtg ttgacttaat tggctacacc ccgtggggct gcattgacct ggtttctgcc
     4741 ggaacagggg aaatgaaaaa gcgctacgga atgatttatg tcgataaaga caacgaaggg
     4801 aagggaacgc tggaacggat acgtaaagcg tcgttttact ggtatcggga tctcatcgcc
     4861 aacaatggcg aaaatatttg agacttgagg aagatattac ggagatgtca ggctctgtga
     4921 tcagagcctg acaataacta catagcgtgt tgattataat agggcacgcc gagcgcatcc
     4981 gacttatcac tgccagtacc catgtgattt aaatcaaacg gtggttgttc ctgcgatggg
     5041 ggaacaacca cgacattggc acaagaagta acaactggct gcgtggtttg ttccgcaacg
     5101 ccctgcgcgg aaaagagaat aagcgcagct gctattgcgg atgaaagtgg tttcatgatt
     5161 gcctcagtga cgttgtcacg ttatcaacta gctgttgtaa tgatttaatg gatgtaaaaa
     5221 gcgcgattcg ccctgcatat tggtcatccg gtatttatgc ggcggaacat caaagtaatt
     5281 tttgaaggtt cgggtcagag tttgttgcga ctcgaagcca tatcgttctg ccagatagag
     5341 tatcggctcg ttactttcct tcagcttttg cgcgatttcc gtcatcttac ggctgcggat
     5401 gtattggcct aatgaatgac cggtttcttt tttaaacatc cgttgcaggt gccatttaga
     5461 gtaacctgaa cgctctgaca ctttctccag tgacagtggc gattccaggt tgtcctcgat
     5521 ccagtccaaa atgctatgaa tggtaatagc gtcagtattg cgtctggaca tcgtcatacc
     5581 tcttttttgt ttacggcagg actttcttaa gcaaatgctc aagtgttgcc acttcgtccg
     5641 ccgtcaggtt ttttgttaat tcttgatgca ggtcctggcc aactaattga tggcattgtt
     5701 cgcatattgc cgcgccgctg gtggtaagtt ttaccagtac gccgcgctta tcattcgggt
     5761 tcggcaacct ttcaacccag cctttacaga ccaggcgatc cagcatacgg gtgagtgctc
     5821 ccaggtcgac cgacaacact tttttcagtt caaccggagt aatacacgcc gcgcagcgga
     5881 tagagcagag caccttaaac tgtgccgcgg taatatccag cggagacaga tactcgttaa
     5941 gcagacgatc tttcttctga ttaaccatat ggattaagcg acccaatgga ataatttcat
     6001 tgaacagatc gctggtactt ttcacattag ttgccctggc aagtaattag ttgcagggga
     6061 taatattgcc taggcaagta taagtcaaat gaatgaattg accgatgcca cgttttgcta
     6121 aatcggttca agttttggtc aatgcgatgc aaggttagct aaagagtgac actttatcct
     6181 tgtttacccc ttatactttt cgctgataac ccagatacac aggataacaa ccaccaatgt
     6241 tagatttgtt taaagcaatt ggcctggggc tggtggtgtt actgccgtta gctaacccat
     6301 taacaactgt cgcgttattt cttggcctgg caggcaacat gaacagtgcc gaacgtaatc
     6361 gtcagtcgtt gatggcctcg gtgtacgtat ttgccatcat gatggtggcg tattacgccg
     6421 ggcaactggt gatggataca tttggcattt cgattcccgg tctgcgaatt gcaggcggct
     6481 taatcgtcgc gtttatcggt tttcggatgc tctttccgca acagaaagcg attgattcac
     6541 cggaggcgaa aagcaagtcg gaagagctgg aagatgaacc cagtgccaat attgcctttg
     6601 tgccgctagc aatgccaagt accgctggtc cgggaaccat agcgatgatt attagctcgg
     6661 cgtccacagt gcgtcagagt tcaaccttcg ctgattgggt actgatggtt gcgccgccgc
     6721 tgatcttttt cctggtggcg gttattttgt ggggaagcct acgcagctcc ggcgcaatta
     6781 tgcggctggt gggcaagggt ggcattgaag cgatctcccg tctgatggga ttcctgctgg
     6841 tatgtatggg tgtgcagttt attattaacg gcatcctgga aatcattaaa acgtaccatt
     6901 aaaaaaggcc cgaatgggcc tttcaactat tgcgtctgtt cttcgagtgt cactggccag
     6961 cggcgaaata taatgattga ccaaattaac gcggcaaaag caggcaccgc gcccacataa
     7021 ccaatcatcg acattgacca gtgcaaactc acctgattac ctaccaacgc acccgccccg
     7081 attccaatat taaatatgcc ggagaatagc gccatcgcga cgtcggtagc atctggtgcc
     7141 agcgccagca ctttaacctg cataccaagc ccgatgatca tcatcgcgat cccccagaaa
     7201 atactcagca ccccgaggtg tatttcactg ttcgccgcag gtaacagcaa tgccaggcac
     7261 accagcaaca gcgcaatcgc cgtactcacc aacgcagacg catactgatt acccagttta
     7321 ccgaaaatca cgctgccaat aatgcccgaa ccaccgagta ataacagtaa tgccgtggca
     7381 aagttggcgc tgaatcccgc aatgttttgc acaaaaggct cgatatagct gtatgccgtg
     7441 taatgggcgg tgacaaccac cacagttaac aaataaatgc tcatcaatgc cgggcggcgg
     7501 aacaatagcg ggaggctttt caatgaaccg gaatgctcac tgggcagtaa gggaagtaac
     7561 ttaatcaggc acaaaagggt gataagcgcc ccaataccaa tcgcgaagaa ggtcattcgc
     7621 caaccgaaat actggcccac aatgcgcccg agaggtaaac ctaagaccat cgccagtgct
     7681 gtaccggtgg caattaaact caaggcctgt gcccgcttcc cggccggagc catacggatc
     7741 gccagagacg ccgtaatcga ccagaaaatc gcatgtgcaa aagccacacc aatgcgactg
     7801 atcaccagaa cggtaaagct ccacgataaa aacgacagta cgtggctggc aataaacacc
     7861 acaaacaggc agatcagtaa tttgcgccgt tcaacctggc tggtcattaa cataaaaggc
     7921 aatgacatta gcgccactac ccatgcataa atggtcaaca taatgccgac ctgagcggtt
     7981 tgcatatgaa aactttgcgc aatgtcagag agcaggccaa cagggacaaa ttcggtggtg
     8041 ttgaagatga aggcggcgac tgccagcgta acgacccgta gccacgccac tttgcgggaa
     8101 acagtgtttg ttgtcataac agattatcgg gtgagttgcg aaaaagtgat gaaatgatct
     8161 taaaacggtt aatgatgtga atacaatgca tttgtgatct ggatcgcgtt ttccgtttct
     8221 ctgacgctga ttctgttccg cgtcagagag tcggtaacat cacggtttaa tgacgttcag
     8281 ggaggtctgg cgttttggcg gttgcgggta taggtggtct gacaggggag cgaggcctgt
     8341 ttttcaccag gtcttcgtga gtaacttttg gcggcgtttc attttttaca aacagtttga
     8401 gggtcctgcg cgccatatgt ttgataattt tatcatcact taatgatgct ggcatcaaca
     8461 cgtcattcaa atcactttca ttgggattca taaattggtg atagtgcaat aacatattgg
     8521 cgaaaaataa aaacaagtcc tgaaaatgag gagattcttg ttctgctaaa agggataaaa
     8581 agcagatgca gacttcgggt agtacatgtt tgttagaacc taatgaatcc atcaggcttt
     8641 gcaataaatc ctcttttaat tcgctgtccg ttatgtcgag aatggcggtg atcaagttag
     8701 gatctttatt gacgaaccat ctgtgaacct cagagttatt ccataatcgg gggagtatga
     8761 caaaattacc gcgagagata aaattcagga caagcaaatc aaactggcgt aagtaatctg
     8821 cagcggtatg gcgatccgga ggcaggggtt caatatgtgg tgtatgcgct cttttgatgg
     8881 gaacgccaag gttgaacatt ggaaattgtt caataaaatc attcagaaac agaaatagca
     8941 cacgctcgac gccggtctga aatacgcgcc ccatagtgac aggctttggg ttgggagcgc
     9001 aaaaatatta atttctccat catggcgaaa attatctaaa taaatagggc ttaaatttat
     9061 tactacctga aattcaatat tttctttgtg tc
//
LOCUS       DAZFYC010000167         9034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFYC010000167 DAZFYC010000000
VERSION     DAZFYC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.168"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            148..884
                     /locus_tag="ACHKW5_004642"
                     /pseudo
     CDS             148..884
                     /locus_tag="ACHKW5_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1063..1359
                     /locus_tag="ACHKW5_004643"
     CDS             1063..1359
                     /locus_tag="ACHKW5_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7513868.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1667..1783
                     /locus_tag="ACHKW5_004644"
     CDS             1667..1783
                     /locus_tag="ACHKW5_004644"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513869.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2002..3435
                     /locus_tag="ACHKW5_004645"
     CDS             2002..3435
                     /locus_tag="ACHKW5_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO7513870.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4256..4819
                     /locus_tag="ACHKW5_004646"
     CDS             4256..4819
                     /locus_tag="ACHKW5_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7513871.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            4974..7334
                     /locus_tag="ACHKW5_004647"
     CDS             4974..7334
                     /locus_tag="ACHKW5_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7513872.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLLRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(7496..7672)
                     /locus_tag="ACHKW5_004648"
     CDS             complement(7496..7672)
                     /locus_tag="ACHKW5_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513873.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(7810..8052)
                     /locus_tag="ACHKW5_004649"
                     /pseudo
     CDS             complement(7810..8052)
                     /locus_tag="ACHKW5_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8091..>9034)
                     /locus_tag="ACHKW5_004650"
     CDS             complement(8091..>9034)
                     /locus_tag="ACHKW5_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO7513874.1"
                     /translation="QSEIYGRQGVELRRSLLSGWVDACCRLLSPLEEALHGYVMTDGK
                     LHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHL
                     ACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQ
                     LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQ
                     WPALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKL
                     NDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         2435 a         1900 c         2177 g         2522 t
ORIGIN      
        1 tgcttttctg ttaactggtt ttcatttttg aatcaatatt ttctgaacag ggtaatagta
       61 cacgtattaa cagattatgt cagaaggccc cggtgatgcc ttctgacgat agattaatgt
      121 tgtattctat tggtatctgg agaccatatg gcaacagtta cagtatattc tccccgctgt
      181 aattcctata aagtgtaccg ttatgatcac agcacctcag atcatgagcg tttccagtgc
      241 taatcctgca aacgtgtatt tcaactgaat tacatctacg aaacccgaaa accaggcatt
      301 aaagagcaaa ttgttgaaat ggttcataac ggagccgggg ttcgcgatag ctccagaacg
      361 ctgaaggtgg atatcaacac cgtcattctc actttaaaaa acgctcacca cgtaaagtaa
      421 ccatatctcc agccgccagt gtcgatgtgg cacttatctg tgaacttgat gaacaatgga
      481 gttttgtcga aaacaaagct cgtcagcagt ggcactggta cgcgtataag accaaagctg
      541 acggtgtgct ggcttacact tttggtcctc gcactgatga aacatgccgt gagttgccgg
      601 aattcctgaa gccattctcc gcaggcatga taaccagaga caaccggagc agttatacca
      661 gagaaatgcc acaggataag catcttgtcg gcaagatatt tacccggcgc attgagcgca
      721 acaatctgac acttcgtact cacatcaaac gacctgcccg gaaaacgatt tgctttttac
      781 gctccctgga aattcacgaa aagccattgg ttcatttatc gaaaacacat gtactactga
      841 caggagtcat taccagggcc tcttttgctg tttttttgcc atgaatgata atgagaatcg
      901 acagcgataa gggaaaagta aaataacact aatgctgtcg ttttctatca tattgatttt
      961 taattattta ttgtcttttt tctcaaccca tttttttacg cagacaaatt tacccgggga
     1021 atacaaactg taacgacagt tcaccacact atgggggata aaatgccaga tcaatccgga
     1081 cactgcagga ctccttcacg catcttacgg atgcctgaac tgagccaatt gttaggtata
     1141 tctcgttcga caatttacga aaaaatgaat ccgttatcga aatactacga tgcaaccttc
     1201 cccaggccgg tacgtcttgg tagtggttct gtgggctggc gttcatcagc tattgacgaa
     1261 tggcttactt tacatactgt acctgccaga tcagcagtca aaggtgttaa tgacgaagtg
     1321 agacatacgc tttcttcaga tatgaccatg agcagataag tttttgtcat cacgtgatca
     1381 cctcaggagg aaaaagaagc ataagaacaa tttagccaga agtttaattg ataaacagaa
     1441 tttccagcat tcattgttga aagagcagct attgtcgcta ttgtagcggt aattgctgct
     1501 ataaccgaaa cgtattacga aaaaggaagc atttatgcct tacatcaaac cggatatgtc
     1561 gtattcagta tatgcatgac catcaatgat agggaaggca ataattaaag ctcaatacaa
     1621 taaacaagta cctctgactc tggttgcaat gtcagtatta acagcgatgt caattgcctg
     1681 tcagaatcag gttgacgtat gcagtcccgg aaatttacgt ggggccgtta atatttacac
     1741 tatggttctg gagaaaggga aaagaccggg ggaaaggtat tagtgaaata atttccggag
     1801 gtattaagaa tgagaaatat gaatgatatt tcgtggtgaa taatacagtc ttcactgaat
     1861 taaccttatc ggtttattaa acatcagatc tacaaatgct ctgtatttct cagagcatca
     1921 ggtctgaata gcagctattt tcgctattgt agcgggattt gctgctattt tactaaccca
     1981 ttaatgaaaa gagaggcttt tatgtgctta cttgcaccag aaaatcctta tccgatatat
     2041 gcactaccac cgctggtgag aaatgcaata attgaaactc aaaaaaatac acaggcacct
     2101 ttggctatgg tggcaacatc cgcattaaca gcgatctcaa ttgcctgtca gaatcagatt
     2161 gacgtgtgca gacctggaaa tttacatggg cctgttaatc tttactctct gattctggct
     2221 gattccggtg aaaggaaaac gaccgtggat aaagtgttta tgaaagcatt ttatctcagg
     2281 gatgaagccc tggcggatga atacgcgaaa ctggttgaga attacagtac agaaaaggaa
     2341 atatgggagc aaaaacaaaa agcgctggaa tcaaaatttc ataaagagat tcgtgccggt
     2401 aaagattaca aggcaacaga atcagagctt gaaacgcatc tgaataaacc tcctgttccg
     2461 ccgcagatac gtcgaacaat ttttaatgag acaacgatag agggaatgtt aaaatattac
     2521 tccgatagca atcgctcttt tgctcttgta tccagtgaag ggggggtaat ttttgacagc
     2581 cgggccatga gtaaactggg aattattaac actttgtggg atggaggttc tcttttcatc
     2641 gacaggaaat catctcccgg aattaatttg aaggagccaa gactgacgat atcggtgatg
     2701 attcagcctg atgtttacca caaaggtttt tgtacgcgaa aaaaagaaat tgtgaaaacg
     2761 tcaggacatc atgcaaggtt tttgatgtgt caaccaacat caacgcaggg gacaaggata
     2821 ataaccggag ataattattc atcacagtat caggatttgt ttgaagagag gatcaatgag
     2881 cttattgatg aaagtctggc gatgagtggt gaacgacgtt gccttcactt ttctcctcag
     2941 gctgccagaa tctggacgga ttattacaat gatgtggaat cgaagctggg gggattgggg
     3001 cctttaagac attgccggga atatgctgct aaaaatgcag agtatatggc aagactggca
     3061 ggacttattt accattcgag cggtgaagag ggggagattt ccccttacac tgcagaaatg
     3121 gcgagagaat tagcaatatg gtacggtaat gagtatgtgc ggttgtctaa tccgttaact
     3181 tttgacaact ctgccctgac cgtacctgtg cgactcattc cagaggagct tgaacttttc
     3241 aactggataa aaagctattg tattgagaag gggatcctct gtatgaaaaa aaatgacatt
     3301 ttacagcgtg gtccgaaccg tttccggaaa aaggataaaa tcaactggtt actggattta
     3361 ctgtatgaac aaaacagagt tgtaccggtt attgagggaa aaacgttgtg tgtcgcacct
     3421 aactttgacc tctgaacgac ttaatcgttg agtacatata gtctcctctg ctacttgcgg
     3481 aggagactaa tgtctttacc atgtcagcgt tgtattagaa aactctggta caggtcagtc
     3541 agtaaatata actggtgtaa ccactattta ctattggata gagtgttact caggatattg
     3601 tcggctttag tctgaggtga ggtggggcct tcattgtttt cgtgttcaat aaataattta
     3661 ggttaattag tattattagt ggaagttatg tgatctgtta tgttgtcttg tacgcactta
     3721 acactggtta cgtaccggaa cactattaga gtatgttatg gctctgttac agtttatagg
     3781 tatgaaaaaa tataatcttt cctgtatgaa ataatattct tatggtgtgt ggggtatggt
     3841 ctgtatacca tcatttttat ggtgaagtta aatatctgta gaggcctcaa gctgggtcgc
     3901 tgtttttact taatggtaca gaagtattaa tagcggtgtt gcctgtggct acccgatact
     3961 ggcttttgtg aatgtcagat tacttcctgt aagtgattga atggtacttc cttaaactca
     4021 attctatgag gtgtgttttt accattgtaa ttcgtactgg tcctcagttt aagtgagtct
     4081 tactgtactc tgaaatatta cctctttgga gtaatgaggt aatacgaaac agttttatca
     4141 ggttgtctgt gctgctggtt acctcgttta ttaatgccgc ggtacggcgt ttctgtactc
     4201 tcagtattta tgtaattcca ccttcctgta acttttataa agtaaggact ataaaatgta
     4261 taatgctaat cccaattacg aaatggactt catgattctg aaagacgtga atgaacacat
     4321 ggaaggtctg tttcaacgtt tttctaaact gttacctttc agaattgatt ttgcctacag
     4381 gaaagacacg cccagttttg gtcattcctg taggcacagt atgtgtattg aaatgcaccg
     4441 cttacttagt gaaacccaaa cgatgctggc gggatattac tgggtaatgg aatatacgtc
     4501 aaacaaggga cttcatattc attttatcgg ctacctggat gggcaacgtc acaacaaatc
     4561 ttatcggata tccaggcaac tgggagatat ctggaggcgg atcacggaag gggagggtta
     4621 ttttcacctg tgcagggcta aagacaaata cccggtgcgt atcgatcacg taattcacta
     4681 tagtgataaa tctgcggtag acgacttacg ttacgccctt agctatctgg ccaaacagga
     4741 tcagaaagaa cacggtatta ttctgaggcg tagcaggctt cccgaaaaaa gtaaccgggg
     4801 ccgaccacga cataactgaa ttacagctgt attaggtatt agaattaagt ttctgtaagt
     4861 tgttgggagg tgcttgagtt aataggcaga agagggcggg gcgctttgtc atcagtgtag
     4921 gggacccgaa tttctgccgt ggtgcctcag tggatcggat acaggtaacg aatatggttc
     4981 ataaatctga cagtgatgaa ttagctgcat tgagggcaga aaatgcccgt ctggtctcat
     5041 tacttgaagc tcatgggatt gaatggcgac gtaaaccgca gactcctgtg cagcgcgttt
     5101 ctgtattatc caccgacgag aaggttgcat tatttcgtcg gttgtttcgt gggcgtgatg
     5161 atgtatgggc acttagatgg gaaagtaaaa ccagcggcaa atcagggtac tctcctgcct
     5221 gcgctaacga atggcaggcg agaatatgcg gtaaaccccg gataaaatgt ggggactgcg
     5281 ctcaccgtca gttgattcct gtatccgatc tcgtcatcta ccaccatctg gccggtaccc
     5341 atactgtcgg gatgtatccg ttgctggaag atgattcctg ttattttctg gcagttgatt
     5401 ttgatgaagc tgaatggcag aaggatgcat ccgcattcat gcgatcctgt gatgagctgg
     5461 gtgtacctgc tgcgctggaa atatcccgtt cacgtcaggg ggcgcatgtc tggatatttt
     5521 ttgcctcacg agtttcggcc cgcgaagctc gccgtctggg gactgctatt attagctata
     5581 cgtgtagtcg gacccgacag ctgcgattgg ggtcttatga ccgattattt cctaatcagg
     5641 atactatgcc aaaaggggga tttggtaatc tcatagcgtt acctctgcaa aaaagaccgc
     5701 gtgaattggg gggaagcgtt tttgttgata tgaatctcca gccttatcct gatcagtggg
     5761 cttttcttgt atcggtgacc ccgatgaatg tgcaggatat tgaaccgacg atattacggg
     5821 ctacagggag tatccatcct ctggatgtga attttatcaa cgaagaagac ctgggtacgc
     5881 cgtgggaaga gaaaaaatca tcaggaaaca gactgaatat ttctattgca gaaccgctga
     5941 aaatcacgct ggcaaaccag atctatttcg aaaaagcgca attacctcag gtgctgatta
     6001 accgacttat tcggctggca gcatttccga accctgagtt ttataaggct caggcaatgc
     6061 gtatgtcagt ctggaataag ccccgtgtta taggttgtgc ggagaattac ccgcaacaca
     6121 ttgcgttgcc ccggggatgt ctggacagcg tattatcttt ccttagggac aacaatattg
     6181 ctgcagaatt aatcgataaa cgatttgccg ggacggaatg taatgccgtt tttatgggaa
     6241 acctcagagc ggagcaggaa gaggccgttt cggcattact ccgttatgac actggtgtgc
     6301 tttgtgcgcc aacggctttt ggtaagacag ttaccgcagc ggcagtgatt gccaagcgga
     6361 aagtgaatac actgatactg gtacaccgga ctgaattgct gaagcagtgg caggagcgtc
     6421 tcgcggtgtt tctgcaggcc ggtgacagta ttggtattat cgggggaggt aaacataaac
     6481 cctgtggcaa tattgatatt gcggtggtgc agtccatatc cagacaagga gaagttgaac
     6541 ctctggtcag gaattatggg caaatcattg tggatgagtg ccatcatatt ggcgcggttt
     6601 cattttctgc gattctgaag gaaacgaatg ccagatatct gcttggcctg acggcaacac
     6661 caatccgacg ggatggtctg catcccatta tttttatgta ctgtggtgcc attcgccata
     6721 cagcggtccg cccgaaggaa agcccacata atctggaggt actgctccgt tcccgtttta
     6781 catctggtca tttaccatcg gatgcgagaa tccaggatat tttcagagaa attgctctgg
     6841 atcatgacag aacggtggcg atagctgaag aagccatgaa agctttcggg caggggcgaa
     6901 aagttctggt actgactgaa cgtacagatc atctggatga gatagcatca gtgatgaatt
     6961 cactgaaatt gtctcccttt attctccatg gtcgactatc gaaaaaaaag cgtgcgatgc
     7021 tgatatccgg gctgaatgct cttcctcccg attctcctcg aattttgttg tcaacaggca
     7081 gacttattgg tgagggattt gaccaccctc cgctggatac gctgattctt gccatgcctg
     7141 tgtcatggaa agggacatta cagcagtatg cagggcgtct tcacagagag cataccggta
     7201 aaagcgatgt caggatcatt gattttgtgg ataccgcgta tcctgtgctg ctcagaatgt
     7261 gggataaacg tcagcggggt tataaagcga tggggtacag gattatagct gacggcgatg
     7321 aatcagtgat ttaattttaa ggagtacttt acccttttta aataatttga gctccccggg
     7381 ttgttcgggc aacaaagagt cccagtatac aggcttcagg aatatgttgt agtatcaggc
     7441 ttttcatcgc tttaaaatga ctaccggcag tgagaacgtc atcaaaaata acaatctagg
     7501 gcctgcaccc ttccagtaat tgttcatcca gttggtagtt tgccataatc gcttctggta
     7561 agggccggtt tgttgcggta tgagaggcta ttgttgatac gttttgcaca accagttccc
     7621 agaaatccag ccatggttcg taggtttgcg caattcgcag aatttctgtc atacggtcat
     7681 cgtaagcggc atctgtacgg catttggatg gtggtacggg aacaaatgtg tattcctcaa
     7741 gattaccgat atttgatatg agcatctgag caacatactc aatagcagcc tttttatagt
     7801 ggtaatcagg ctacgaaagt acagcgtttt ataaattgtc tcaggtgata ttcgcagcgt
     7861 tttttgacgt ggttttgttc gccttaacca tcctgatatt tgctctggag accatttcat
     7921 ctccagcttt tccagaacaa gctttcgcaa tggtaaattt tgatccagta agcacggttt
     7981 tggccttttc gccattctgt tggctcggtt attagcatca acagctttgt aatagcgtct
     8041 gccccgatta cgcgtaagcg tacagcgagg gccgtattga cggggatgtg ttattcagct
     8101 ggcagtgcta tgcgccacgg aagcagttcg ctgacccggt tgaccggcca gtctgctatg
     8161 acgccaggca catggcgaag gtagctttct ggatccacgt cattcagttt gcacgtcccg
     8221 atcaggctgt acagtagcgc tccccgctca ccaccatgat cagagccgaa gaacaggaag
     8281 tttttacgac ccagactgac cgcccgcagg gcattttcag cgatgttgtt gtcgatttcc
     8341 acccagccat cgttcgcata gtacgtcagt gccggccact ggttaagtgc gtacgcgaac
     8401 gccttcgcca actctgagtg tcgcgacagg gtcttcatct tttcacgcaa ccagctttcc
     8461 agggatttca acaacggttt cgtttttcgc tgacgttcag caagccgctg ctctgccggc
     8521 attcccctta tatccgcctc tatggcgtac aactgaccga tctgctccag ggcttcttcc
     8581 gtcagtgctg acgggatgcg gacgtgcaca tcgtggatct ttcggcgggc atgagcccag
     8641 caggcagctt ccgttatccc accattgcga tacagctcgt tgaacccggc gtacgcatcc
     8701 gcttgcagca caccgctgaa gcaggcaaga tgagtctgcg gatggatgcc ttttctgtcc
     8761 gggctgtaag cgaaccacac tgcaggtgcc aacgctgacc ctgcattgcg gtcatcacga
     8821 acatacgccc acaaccgccc ggtcttcgtc ttcttattac ccggcagcag tacctggacc
     8881 ggggtatcat cggcatggag tttgccgtca gtcatgacat agccatgaag cgcctcttcc
     8941 agcggagaca gcagccggca gcatgcatcc acccagcccg acagcagtga acgcctcagc
     9001 tccacacctt gccggccgta tatttctgac tggc
//
LOCUS       DAZFYC010000168         8872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFYC010000168 DAZFYC010000000
VERSION     DAZFYC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.169"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            284..934
                     /gene="csgD"
                     /locus_tag="ACHKW5_004651"
     CDS             284..934
                     /gene="csgD"
                     /locus_tag="ACHKW5_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO7513875.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPENYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            939..1328
                     /gene="csgE"
                     /locus_tag="ACHKW5_004652"
     CDS             939..1328
                     /gene="csgE"
                     /locus_tag="ACHKW5_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO7513876.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1353..1769
                     /gene="csgF"
                     /locus_tag="ACHKW5_004653"
     CDS             1353..1769
                     /gene="csgF"
                     /locus_tag="ACHKW5_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO7513877.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1796..2629
                     /gene="csgG"
                     /locus_tag="ACHKW5_004654"
     CDS             1796..2629
                     /gene="csgG"
                     /locus_tag="ACHKW5_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO7513878.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2693..3184)
                     /gene="ycdZ"
                     /locus_tag="ACHKW5_004655"
     CDS             complement(2693..3184)
                     /gene="ycdZ"
                     /locus_tag="ACHKW5_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7513879.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3286..3840)
                     /gene="ycdY"
                     /locus_tag="ACHKW5_004656"
     CDS             complement(3286..3840)
                     /gene="ycdY"
                     /locus_tag="ACHKW5_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO7513880.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3864..4601)
                     /gene="ycdX"
                     /locus_tag="ACHKW5_004657"
     CDS             complement(3864..4601)
                     /gene="ycdX"
                     /locus_tag="ACHKW5_004657"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO7513881.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4656..5594)
                     /gene="ghrA"
                     /locus_tag="ACHKW5_004658"
     CDS             complement(4656..5594)
                     /gene="ghrA"
                     /locus_tag="ACHKW5_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO7513882.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5828..5915
                     /locus_tag="ACHKW5_004659"
     tRNA            5828..5915
                     /locus_tag="ACHKW5_004659"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5862..5864,aa:Ser,seq:gga)
     gene            complement(6065..6907)
                     /locus_tag="ACHKW5_004660"
     CDS             complement(6065..6907)
                     /locus_tag="ACHKW5_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7513883.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6992..7189)
                     /locus_tag="ACHKW5_004661"
     CDS             complement(6992..7189)
                     /locus_tag="ACHKW5_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7513884.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7201..7692)
                     /locus_tag="ACHKW5_004662"
     CDS             complement(7201..7692)
                     /locus_tag="ACHKW5_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7513885.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7689..8063)
                     /locus_tag="ACHKW5_004663"
     CDS             complement(7689..8063)
                     /locus_tag="ACHKW5_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7513886.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8110..8484)
                     /locus_tag="ACHKW5_004664"
     CDS             complement(8110..8484)
                     /locus_tag="ACHKW5_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7513887.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8647..8868)
                     /locus_tag="ACHKW5_004665"
     CDS             complement(8647..8868)
                     /locus_tag="ACHKW5_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7513888.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
BASE COUNT         2308 a         2172 c         2127 g         2265 t
ORIGIN      
        1 ataaaaacca aatgtacaac ttttctatca tttctaaact taataaaacc ttaagattaa
       61 cattttaata taacgagtta catttagtta catgtttaac acttgattta agatttgtaa
      121 tggctagatt gaaatcagat gtaatccatt agttttatat tttacccatt tagggctgat
      181 ttattactac acacagcagt gcaacatctg tcagtacttc tggtgcttct attttagagg
      241 cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc atcatgttta atgaagtcca
      301 tagtattcat ggtcatacat tattgttgat cactaaaccg tctttgcaag cgacagctct
      361 cttgcagcac cttaaacaat cgctggcaat tacaggaaaa ttacataata ttcaacgttc
      421 tctggacgat atatcttcag gctctattat tcttctggat atgatggaag cagataaaaa
      481 gcttatccat tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt
      541 gctaaatacg cctgaaaatt acccgtaccg cgacattgaa aactggcctc atatcaacgg
      601 cgttttttat gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg
      661 cggcgaatgc tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta
      721 tcgttataac agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa
      781 gctgcgtatc ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac
      841 ggttaaaacg catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc
      901 ggtttcctgg gcaaacgata acctgaggcg ataaagccat gaaacgttat ttacgctgga
      961 ttgtggcggc agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc
     1021 cgggattgtt aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta
     1081 gtgataaatg ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac
     1141 gatggggaag ctggatcact ataacggtca atcaggacgt tattttccag acttttttat
     1201 ttccgttgaa aagagacttc gagaaaactg tcgtctttgc actgattcaa actgaagaag
     1261 cactaaatcg tcgccagata aatcaggcgt tattaagtac gggcgatttg gcgcatgatg
     1321 aattctaaat aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac
     1381 tcatgcttat ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa
     1441 actttggtgg taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact
     1501 cttataaaga tccgagctat aacgatgact ttggtattga aacaccctca gcgttagata
     1561 actttactca ggccatccag tcacaaattt taggtgggct actgtcgaat attaataccg
     1621 gtaaaccggg ccgcatggtg accaacgatt atattgttga tattgctaac cgcgatggtc
     1681 aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg
     1741 gtttacaaaa taactcaacc gatttttaag ccccagcttc ataaggaaaa taatcatgca
     1801 gcgcttattt cttttggttg ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa
     1861 agaagccgcc agaccgacat taatgcctcg agctcagagc tacaaagatt tgacccatct
     1921 gccagcgccg acgggtaaga tctttgtttc ggtatacaac attcaggacg aaaccgggca
     1981 atttaaaccc tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg ccacggcaat
     2041 gctggtcacg gcattgaaag attctcgctg gtttataccg ctggagcgcc agggcttaca
     2101 aaacctgctt aacgagcgca agattattcg tgcggcacaa gaaaacggca cggttgccat
     2161 taataaccga atcccgctgc aatctttaac ggcagcaaat attatggttg aaggttcgat
     2221 tatcggttat gaaagcaacg ttaaatcggg cggtgttggg gcaagatatt ttgggatcgg
     2281 tgcagacacg caataccagc tcgatcagat tgccgtgaac ctgcgcgtcg tcaatgtgag
     2341 taccggtgag atcctctcct cggtgaacac cagtaagacg atactttcct atgaagttca
     2401 ggccggggtt ttccgcttta ttgactacca gcgcttgctg gaaggggaag tgggttacac
     2461 ctcaaacgaa cccgttatgc tgtgtctgat gtcggctatc gaaacagggg tcattttcct
     2521 gattaatgat ggtatcgacc gtggtctgtg ggatttgcag aacaaagcag aacggcagaa
     2581 cgacattctg gtgaaatacc gccatatgtc ggttccaccg gaatcctgac agataaaaaa
     2641 gccggaatta tcatccggca ttttactgtt taagcgaaag cgtgagtgcc cctcacgctt
     2701 tatttttgat ttcctgctca cggtgagccg tctttgcact acgcgccgcc agccacaggc
     2761 cgctgttttt cattgcgtaa ccaaatacca gcccaagcgc cagcgaaggt aacaccagtt
     2821 tccagtcacc ctgcccggca aacgtcgcgc atgcgcctat aaacgtcccc ggaacaaatg
     2881 aaagcagcag ctgtttggcc tgaatacaca tcagaaacgc gacaataccg gttatgacat
     2941 aaccgagaat ttccagatgt ggtgccagtg cactaccgta aataatgacc atcgcccaca
     3001 ccacgccact aagcagggtt gcagcggaga tcgccagccc tttcagccca ccttgcgggc
     3061 aggcaaagta ggccgtacag cccaggaagc ctgcccagct tagtaagccc agggaaacag
     3121 ccacccatcc ccagataccg gagagaatgc ctgttgtgat tgcaatagag agaagtatgt
     3181 tcatgccgcg catgatagca gaaaacgcgg ccatgaatga gattgagcac acattttatg
     3241 caacaacaaa ttatctgttg cagttatgag tgacgatgat cacatttact cttcagaatc
     3301 ttcttccagc tcgtcccaca ttgcactaat ggcatcacgg gttagcggtg ccatggtgcg
     3361 ccagaaaggc gtggttgcat gggcctccac tttgccgagg aacgcaccac accagggtaa
     3421 cagatactca ctgaacagtg tttccagtgc ttcgctctca tcttccgttg actgatcttc
     3481 cagccaggaa gccgcaagca gcaatgtgcc gatgtgatcg gctggcgtat ccgccagtgg
     3541 catccctcgt tcggaaagaa aagcgcgcac ttccgcttcc gttgcgtcct caacccatgc
     3601 gctacgatat ggcggcacgg cacattcatc gccgataaac aacgcattgt aatcggcaga
     3661 gacttgcgcc atatcacagc ttttctgtaa acgcgtcagt aactcatcct gctccagtgg
     3721 ccagttcgca gccagtttcc cctcacgaat cagggtaaac agcggcacca gtaaaggatc
     3781 ttgcggttgg cggtaataga gcgaacccag cacacgacag aggatagaaa actcgttcat
     3841 taatatattt ccattactag taattaaagg tctgcaaatt ccgcaatcgg tgccataccg
     3901 cgagattcaa ggaagttcag taagcggcgc ggagaaacat tcaaaatgcg ctctggcgga
     3961 aaatctaccg cgtcgaggat tttaagacac tcttcaaatt cccccatggt aaacgcggtg
     4021 tgagaatccg agcctaatgc cacccaacct ccagcatcac gtaccgctgc ggctaccgca
     4081 cgacagttgt cttcactgcc cttacgtgag tgtaaaaatg aagaattatt gatttccagc
     4141 gccacctgat gtttcgcggc tgcttcagca acggctttca catctatttc atatttggga
     4201 tttccgggat ggcttattat atgcacattg ccgctggcga tagtggcgat catcgcttgt
     4261 gtattggtcg ctttgtcatg tggtgcaaaa accggctcat gaaaaccggc aataatcaga
     4321 tccagcgagt caaacatttt accgctgcaa tcaatttcac catcaacatt tttaatgtta
     4381 gcttcgatgc cgcgcaggat ccctacccca tcaaccactc gcggccagat acgcatgtta
     4441 atgaagtgcc agtgatgcgg agcatcttcc atatccgggc catgatcggt gatcgcaaaa
     4501 agtttaatgc ccttttgttt ggcctgggca atgtaatcac tcaatgtgct atatgcatgt
     4561 gtgctggcaa cggtatgcat atgaaggtcg acgggataca taactctctc ctgtattcaa
     4621 ttcccgccaa ggatagcagg aatcctgatg ctttattagt agccgcgtgc gcggtcgact
     4681 tgcccgcaga ccctctcccc tttttcgagc tgggcaatgg tgcgagaaat gtactccaca
     4741 gcttcagcgg gacgggtaat cgcggcgaca tgtggtgtta tcgtcacgcg tggatgttgc
     4801 cagagcggac tttcaggcgg taagggttca cgattaaaaa catccaacat tgcgccttta
     4861 actttgccgc tatccagcgc cgcgagcagg tcatcttcca caacatgaac accacgcgcc
     4921 aggttgagga gatacgcgcc atccggtaat ttttcgagta attgttgatt aataatgccg
     4981 acggtttcag gggtattcgg taacaaatta atcaataccc gacattggct cagaaatgca
     5041 gacagttctt cccgtccggc aaagctttgc acgccaggcc acgatttacg ggttcgactc
     5101 cagcaacgca gcggaaagcg ccaggtttgc agactctgag caactttact gcccagtacg
     5161 cctgcgccca aaatgccgat ggtaaaatct tcccgatgat attcaggcag cggttgccaa
     5221 tgcgaactat tttgctggat gcgataatcg tcaaaacgtc gaaaccaatg cagcacctga
     5281 ctgacagcat attcctgcat ttgctcgccc ataccggtat cttccaggcg aaaaagtgga
     5341 acagaagggt tcagcatttc agggtgtgcc tgtagcttgc tcaaaataga atcaacaccg
     5401 gccccgagtg cgaacaccgc tttaagatcg cgccctgcca gcatttcaac aggaggatgc
     5461 cagactaaag cataatcagc agagtcatta tctccgcttt tccatgctct gactcttgcc
     5521 tgaggaatag ctttgcgcag tgcctcaatc caccattggg tatcgaacgt tgggtgataa
     5581 aagatgatat ccatactgac tcccgaaaag cgttgtgcga aatttattcg cacttatcgt
     5641 tatgatctac aaaggccacc agcataacaa atccgtggtc ggtggcaaaa aaagcagatt
     5701 tcgcttacta aaaccacaca ttgattgaag tttgaataaa cgcgcgattt tttcaaaaag
     5761 tttgttgacc tcaggtcatg atttccctaa attagcgccc gttccagcaa gacaggaacg
     5821 acaatttggt gaggtgtccg agtggctgaa ggagcacgcc tggaaagtgt gtatacggca
     5881 acgtatcggg ggttcgaatc cccccctcac cgccagatta taagcgcatg ttattgttta
     5941 acatgcgctt ttttattttc ccttccagac agtatgccat cgggtatgcc atctgaaacc
     6001 ggtttatatg ccccgcgatc atatcttatc ggacaatttc cgatctttga gcatactggc
     6061 aaagctatac tgccagcata tcaggatagt gttttgcgat aatatcattc agtctgtcaa
     6121 ccagttctgg ttttctgaac gtgatatgcg cggagccctt cttaaagtat ctgatgctga
     6181 acatctcatc ttcatagctc tctttaccct gaacagactg aatgtgatca tccagacgaa
     6241 gggtgatgtt tccccggttg tcggggattg gtttaccgct gaacaggtgc aacattcttt
     6301 ccagatcggc gagtcggtca gcctgttgtc cggtgataag atgaaatccc caccggtccc
     6361 atctcaccag attgttgacg ataattttac tgccaaattt acaggggcaa ttggttttgt
     6421 aattccagtt cagccctctg aagacgttga tcacccctcg ctcaaacacc tcatccttat
     6481 tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc gctgatttcc ggaaaattat
     6541 cgtactccag tgaccggtac catgtatcac gggcctgcgt atccattaat gacagcatcc
     6601 cggaacgttg catcaggtca cgccagattt cgcgatcaag attccgggta atggctttca
     6661 ttgctgtttc gggtttctcc atcagccagc agccgcagcg aaaatcctgt ttcatggccc
     6721 agtccggggc agttttacct ccgatgcttt ccgtcagcac cgagatttca gccagttgtc
     6781 ggataagtgt gtcgatttgc aacagtgtac tctttcgtac agccagaatg tggcgaatgc
     6841 tggttgagca gataacgtcc gtatgcccgt tgaccacttc cggacgggag attgtgatat
     6901 ctgacataat cgatattcct ttttgcagat aaatgaaaac gccagccctg tgcaggctgg
     6961 cgtcagagga atgaagtctg gtgagtgagc ttcaccggtt ctggtgcaga agctgcagat
     7021 gacgcagggt acggacatcc tcccgggcct gttcgataca gggcaacagt gctgctgaat
     7081 ccgttttatc ctcatcgttg tcaaagataa ttcccgatcc gcagtcgata ttgtcctgca
     7141 gccacgcaat cagaatatcc agcgctgttt ccgtggttaa tgatttcatg ttgttcctgg
     7201 ttaccagtcg aaagtgggta aacccggcag gcattcagcg ctggcatcca gatgaatgag
     7261 actgacgcca taacgccgga tgagtgtgac gaccagacga cggaacgttt ttgacagccc
     7321 caggcgttta agccgcagaa ccgggtatga ccacgcatcg gtacgtaaca gatatccgct
     7381 acctgtaaaa tgaatccatt ctgattcacc gaagtcactg gtctggtgtg acagcgagta
     7441 caaccaggcg ttgtccgttt ccgtgatatg tgcggtactg cagcgtatgc cggtaagggt
     7501 agcaaatggt ggcgtgcatt ctgctgaggt ctggtcagat tcatcaacaa cacgaaggga
     7561 gtagccgtta tcgcagacca tgttaatcag ttcagcgagc tcaataccat cgacgtcaac
     7621 gacaatgcgc cccatgttca gtgcctgtac gttaacgctg tcggcttcca gtgtcagggc
     7681 cagtttcatc atttcgcctc cggatactta cccaaggtaa tgttatttac cgttctgtaa
     7741 ttgtcgcggg tcatcaggcc ggtcgcccgg cgagcccgga ggatatcaat actgtttatt
     7801 aactgagagc ggggacaggt gctgaatccc ggctggtcgg tacgcaccag cgcgtatttt
     7861 tccacgagaa aattaactgc atcacacagt gaaatgcctg cctcaatatg ctgctcaatc
     7921 acacgttcat cggcaaacgg tgtgtcattc agtgtgaggc cataatgctg gtccagcagt
     7981 cggatgagca gtgtctgcca gatggtgacg ggagacgggc agcgagatac ctcccgtaca
     8041 tgtgtgtcgg ataatgtttt catcttaagt tctctgtaac tggtagtgct aaaggtgaga
     8101 gcttcggggt tatgaggtgg ttgcgggtgc tggtgtcgga taaacagcca gataaacgta
     8161 accacaggag ccgagggtgt cggcttcgca ggtcagccct tttgcattca gcgtgacggt
     8221 atgctgatat cggggattca gttcaccgct ggtgagcatg agttccagtt gtttcatcag
     8281 cagcggaaag gcctggtcca gatggtatgc atctgcgtcg ctgaagcggc ctctgatacc
     8341 ggcgcggtcg gcaaggtaat gcaggcggtt accctcttgc accagacgag cgccaaaaca
     8401 gggcctcact gtgcagggaa gcccccacca ggggcggtcg tgattgtcat cgggatgagt
     8461 tatcccggag agtttgtctg acacgataaa atcctctctg aaaagtggtt aagaatggtt
     8521 taacgatgac aggtcagccg gcgcgctgac cggtttcggt gaagatgtat gcattgatga
     8581 gaatggcctc atccacagcg gcgtctgagg tcatctggtc gtactcgttt cccgggtgac
     8641 ggaacatcag ttcagggtga cactcatcag ccggacatac gggccaaaac tgtccttacg
     8701 gcgttctgca aacacggcaa gaacgccggg aatatcctgc acttcacgac cagtatacgc
     8761 ttcagcactg ccgtgccagc gatacttacc ggtacagaac ggaaaaagac gggatgcagg
     8821 atgctgttgg tgaatacgca tggcttcacc acgggtgatg attctcatga tg
//
LOCUS       DAZFYC010000169         8800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFYC010000169 DAZFYC010000000
VERSION     DAZFYC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.170"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            531..875
                     /locus_tag="ACHKW5_004666"
     CDS             531..875
                     /locus_tag="ACHKW5_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513889.1"
                     /translation="MLSKLTSVQFECVSLIEMVRSYQEEAASLKAKIAEIEDFQQQAE
                     GYKLNKLDSGTLVYSKKQFIGDTEITVHLCPQCFGKKVISVLQPMEIMRFDAHFRTFC
                     PACNNKFWMNSR"
     gene            complement(1011..1230)
                     /gene="ydfC"
                     /locus_tag="ACHKW5_004667"
                     /pseudo
     CDS             complement(1011..1230)
                     /gene="ydfC"
                     /locus_tag="ACHKW5_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
     gene            complement(1390..1545)
                     /locus_tag="ACHKW5_004668"
     CDS             complement(1390..1545)
                     /locus_tag="ACHKW5_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7513890.1"
                     /translation="MDTIDLGSNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1748..2155)
                     /locus_tag="ACHKW5_004669"
     CDS             complement(1748..2155)
                     /locus_tag="ACHKW5_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7513891.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPNNQPAILTEDQKELLQLFDALPDS
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2232..2459
                     /locus_tag="ACHKW5_004670"
     CDS             2232..2459
                     /locus_tag="ACHKW5_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7513892.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            2443..2994
                     /locus_tag="ACHKW5_004671"
     CDS             2443..2994
                     /locus_tag="ACHKW5_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7513893.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFDDGAVDQRALGVNRVKIFERWKSIDTRDKREKFTALIPAIMAAIRISDFRLY
                     REITDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKRAYRQQY
                     QHYFADKQISVWGSYEYRCATMG"
     gene            2966..4006
                     /locus_tag="ACHKW5_004672"
     CDS             2966..4006
                     /locus_tag="ACHKW5_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO7513894.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVTEDEYTQKRE
                     SLKVGKIGTVNKNRTENGYVSAQNSPKNGTLCCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAVPPVFLDGLSEPIGKFPMTDNWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQVQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGAHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            3999..4460
                     /locus_tag="ACHKW5_004673"
     CDS             3999..4460
                     /locus_tag="ACHKW5_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7513895.1"
                     /translation="MTEQQIRQVCRQCMDCCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPAGPTPIELLKQEYERRKAAGFV"
     gene            4493..5239
                     /locus_tag="ACHKW5_004674"
     CDS             4493..5239
                     /locus_tag="ACHKW5_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7513896.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELRKAGFVD
                     KSVYTWRVAYNNVQQEQPAPAELSEETTTATVAKISECDLTATVEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDKLPAEPKSVPVTENDGKAFPQ
                     SAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWERVCAALRELNKHRDMVAGICRKSGQ"
     gene            5236..5400
                     /locus_tag="ACHKW5_004675"
     CDS             5236..5400
                     /locus_tag="ACHKW5_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513897.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKARVKGISLILR
                     GNFHQSTKYP"
     gene            5405..5938
                     /locus_tag="ACHKW5_004676"
                     /pseudo
     CDS             5405..5938
                     /locus_tag="ACHKW5_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324330.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6099..6857
                     /gene="paa"
                     /locus_tag="ACHKW5_004677"
     CDS             6099..6857
                     /gene="paa"
                     /locus_tag="ACHKW5_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO7513898.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPEADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            7196..7351
                     /gene="hokD"
                     /locus_tag="ACHKW5_004678"
     CDS             7196..7351
                     /gene="hokD"
                     /locus_tag="ACHKW5_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7513899.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            7572..7832
                     /locus_tag="ACHKW5_004679"
     CDS             7572..7832
                     /locus_tag="ACHKW5_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513900.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKDAGIKAGFSAFEDE"
     gene            7899..8177
                     /locus_tag="ACHKW5_004680"
     CDS             7899..8177
                     /locus_tag="ACHKW5_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513901.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHDPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            8179..8800
                     /locus_tag="ACHKW5_004681"
                     /pseudo
     CDS             8179..8800
                     /locus_tag="ACHKW5_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2372 a         1947 c         2303 g         2178 t
ORIGIN      
        1 gttccgcgtg cgcttagccg cattcaccgc atcacaaaat tcactttaaa aagggcggca
       61 gagcagtcac ggagtaaaac tgataccgcc aaatgtcacc agaaaattga taacagaggg
      121 cgttgtagcg gggttgtcac ttaagcgtat ggtcaacctg acaacccggt gcattttctg
      181 gagcaatgga ggaaacccca gccatactta ccgccgcgcc atttcgcgga ctgccacaac
      241 cggaagcgca cggtcgacga aaatttaacg acaggctatc tatgaaccag ctacctcgcc
      301 gtacgctttc gcgttgtgtg cctgctttta accacgtcag gcgaggtggt tcctgttatt
      361 ccccaacaac aagaaatttg tataatctgg atatccccaa caacgagaag agtatgtaac
      421 gtgatagttg agttctcagc agcaatgtcg gcaatcaaag aaactgcggt aatggcaaag
      481 ggtattctga atgcaaaact caaactgaga taaattccgc tgtcagcgat atgctttcaa
      541 aacttacgtc tgttcagttt gaatgcgtat cgctcattga gatggtgcgc tcttatcagg
      601 aagaagccgc atctctcaaa gcaaaaattg cagagataga agactttcag caacaggcgg
      661 aaggttacaa acttaacaag cttgattctg gaactcttgt gtattccaag aagcaattta
      721 taggcgatac cgagataaca gtgcatcttt gcccacaatg ttttggaaaa aaggtaatat
      781 ctgtactgca accaatggaa ataatgcgct ttgatgctca ttttcgtact ttttgccccg
      841 cttgcaataa taagttctgg atgaactcaa gataattaac cgtttaaata agttgggact
      901 atccagattt ttaaagaacg tgccggatgc tcacccgtgt ccggcgcacg cactccacct
      961 gacccgtaga gaactcctta attaccaacc ttatcctcgt tggttagcta ttaacgcggg
     1021 tatgtaatca ttctggcaat gcttaatgcc gctgcttttt ccaaattggt gatatcctgc
     1081 tccagagcgg acagattttt cagcctgctt agccctggct tcattggccc atttcagatc
     1141 ctgcgctgca ttaattttct ggcgcatcca ctcataaagt tcatcatcgg tatagtctgg
     1201 cgcgatgatg acgggttctc gtttatgcat actgattcct cgcggtgctg tttcgcttat
     1261 cagccgttag attttgccta actggaaagc gcctgtttaa actcactgaa gctgagagcc
     1321 tcttcgcctt cggcaaggtc ttcgaagtat tcttcgtaag ccttttccat gattgtgtcg
     1381 aaatccatat cactcacctg agtttctttc cagccagcga cgggcaccat tttcggtttt
     1441 aaacgttttg cttttggtat acgtcatcgc ggtgaacgtg ccgtcctggt ttgggaacac
     1501 gccgtacacc agagattcgt tgctgccaag atcgatagta tccatgctga cctcatttcc
     1561 ccttaacgcc ggagtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgagtg
     1621 aaatttagaa taacctaagg catatgatca agatttttat gtagaaaaac ctaagttttt
     1681 ttggtgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
     1741 aaaggggtta tttgtttgcg cttcttttgc gagctttaag tagttcttca aaaagtttgt
     1801 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gagtcaggca
     1861 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
     1921 tcgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
     1981 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
     2041 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
     2101 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
     2161 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
     2221 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
     2281 ccaatgccgc aggagttagg ttggcaagca ttgctgcatg gggggaactg gttcctgaag
     2341 gtcgcgcgat gcgcctgcaa gaggcatccg gcggggaact tcagtacgac cccaaagttt
     2401 atgacgaata tcgtaaggca aagcgggcgg ggcggttgaa caatgaaaat cacccctgaa
     2461 caggtttgtg aggctctgga tgcctgggta tgccgaccag gaatgacaca ggagcaggcg
     2521 acgatattaa tcacggaagc attctgggct ctgaaagaac gcccgaacat cgatgttcaa
     2581 cgcgtcacgt ttgatgatgg cgcggttgat caacgggcgc tgggcgttaa ccgggtgaag
     2641 atattcgaac gctggaaatc tatcgacacc agggataagc gggaaaaatt cacggcgctg
     2701 attccggcaa ttatggcggc tatccggatc agtgatttca ggttgtatcg tgaaattact
     2761 gacggaaaaa gcattacgta catgatcgcc gggttaaaca aagaatatgg cgatgtggtg
     2821 gagtcagggc tactttttgc ggatccagct gttgtggaac gtgagactga cgaacttata
     2881 gaaaaagcta ttgctttcaa gcgtgcgtat cgtcagcaat accaacatta ctttgcagat
     2941 aaacaaatat ctgtctgggg ttcgtatgag tatcgatgcg ctacgatggg ctaaaaaggt
     3001 gaaaaccggc agttcatcca gtaagtcagt attgacctgg cttgctgata tgtgcggtgc
     3061 cgatttgtgt gcatacccgt ctgtatctgc actggcagaa gtaacggaac tgaacaaaaa
     3121 gactgtgcag gacagcttac gacacctgat ggagattggg ttaattgttg ataccggtga
     3181 gagaaaaggc cggacaaaac aaattgtggt gtaccgactt atcggtgttg aagaaagtgt
     3241 taccgaggat gaatacaccc aaaaacggga gtctttaaag gtaggtaaaa ttggtactgt
     3301 taataaaaac agaaccgaaa acggttatgt ttcagcacaa aacagcccca aaaacggaac
     3361 tctttgctgc atggaaaata accaaagaca cccaaatttt ccatcaaaga cacccaaaaa
     3421 cggatcacgg aacccaaagg aacccaaaga tttaaacccc acacataacg cacgcgagag
     3481 tgctccgacc agtgagcagg aagttttgtc gttacaggca gtcccccctg tattcctgga
     3541 tggcctgagc gaacccatcg gaaaatttcc gatgaccgat aactggtatc cgtcacggga
     3601 ttttcgacga cgggctgcgt tgtgggggat ggctttgccg gagacagaat tcacacctgc
     3661 tgaacttgcc gcattccggg actactgggc agcggagggg aaagtgttta cgcaggttca
     3721 gtgggagcag aaattcgccc gtcacgtaaa tcacgtcagg gcgcaggtta aaccagtcag
     3781 caagggggta aaccatgcag cagcaccagg tggaaccgca tcacgggcag ttcaggaaat
     3841 tcgggcagca cgtgagcagt gggaacgtga aaacggattt atcagcgacg gaaacggcct
     3901 ggaagctgtg ggagctcatg ggggaggttt attcgaaccg ctggacccag aagaacgggg
     3961 ccgcaccttc gaagctctgg attgcacaga ttggcgcgat gactgagcag caaatccggc
     4021 aggtctgccg ccagtgcatg gactgctgcc gggcgggtga aacatggcct ccggacctgg
     4081 ctgagtttgt ggcgctgatt tcggaaagcg gggccaatcc atttggtctg acggtggatg
     4141 ctgtgatgga ggagtaccgc cgctggcgca atgagtcctg gcgatacgac ggaagtgata
     4201 agtacccgtg gtctcagcct gtgctgtatc acatttgcct cgagatgcgt tcaaagggga
     4261 ttgaacgcca gatgaccgaa ggggagttaa aacggcttgc agaacggcag ctgacgaaat
     4321 gggcaaagca tgttagcaac ggcctgagtg ttccgcctgt ccggcgacaa ctggcagcac
     4381 ccaaacgtcc tgcgggacca acgccaattg agttgctgaa acaggaatat gaacgccgga
     4441 aagcggctgg gtttgtctga tttgagaagt aatttttacc gggaggaaat ttatggagac
     4501 tgtttttgac gcactgaaag cgatgggaaa agccacgtcg gtagagctgg ctgcgcgact
     4561 tgatatcagt cgtgaagaag tgctgaacga gctgtgggaa ctgagaaagg ctggcttcgt
     4621 tgataaaagc gtatacacct ggcgtgtggc ttataacaac gttcagcagg aacagccagc
     4681 gccggcagaa ctgtcggaag aaaccaccac ggcaacagtc gctaaaattt cggagtgcga
     4741 tttaactgcg acggttgaac aacgcggacc acaaacggct gatgagctgg ctacgttgtt
     4801 cggtacaaca tcacgcaaag tggcttcaac gctggcaatg gcaatcagca aaggtcgtct
     4861 gattcgtgta aaccagaacg gtaaatttcg ttactgcatg ccgggcgata agttaccagc
     4921 agagccgaaa tccgtgccgg taacggaaaa tgatggtaaa gcctttcctc agtcagcagg
     4981 tgttgcgtta ccagtacagg aagctgcaac acaggaagat attaaaacag aaactgtggc
     5041 tgatattgtg caatcgttgc catcgtttac tgaaacgcga gcggatgacc tggttttacc
     5101 atcgctgcat atggcaaacc gcgaattgcg tcgggcgaaa agtcatgtcc agaagtggga
     5161 gcgagtctgc gccgcgctgc gggagctgaa caagcaccga gatatggttg ccgggatttg
     5221 tcggaagtcc gggcaatgag cggatggtgc aggcctgaaa ttatgatact aacaatgaag
     5281 gtaaaatgca tcggcagtct gattggtcgt agtgaggcgg cggtcaggat gaaagcccgg
     5341 gttaagggaa taagcctgat tctgcggggt aattttcacc agtcaacaaa atatccgtag
     5401 cgcgataacg gtcaaaaatc atggcgctga cacttttgtg ccactggaga tgactgtacc
     5461 taagttcagg ggagaagaac acgtccggtg ggatggtcgg gccagattta aagggcaggt
     5521 catggctcca gccagtacgc tggcaatgga ggctgcctgg ctggaaattg atatgggaac
     5581 cacgccactc agggatttac tgccggtcca gagaataaat tcctgttacg gttacaccac
     5641 tgtgatcttg caagtgcagg aaagtaggtc tacacggtaa cgcgagtgcg tgtaactttt
     5701 gatgttattt ccgtagaaac accggacaaa ttttcgctgg caggtcatgc tgaaggtata
     5761 aatctgcaga ttatggacaa ttacggatat ccggcaagag ccggaaaaag catgccgcct
     5821 ctaattctca gtggaagatg gacttgatta tactcattgc attgtcagaa atagttatcc
     5881 attaaaggct ggctattcca aacaggatgt tgattacaaa aatgtaatca acatgtaagg
     5941 tttatactct tcaatatgcg tataattttc cttattttgt taactttaaa taacaagcta
     6001 tgcacgaggt aaagtcggat aagtttatct ggatgtaata tatattattt gtagtgttta
     6061 taactttatt tcatgataac caataaaagg agttttttat gaggaacata atggcaggtt
     6121 ttttaatatt cctgtcttct gctgcttatg ctgatatcaa tctgtatggt cctggtggcc
     6181 cgcatacagc cttgcttgat gcagccaaac tttatgccga aaaaacaggt attatagtga
     6241 acgttcatta cggcccacag aacaaatgga atgaagatgc caaaaaaaat gcagatatct
     6301 tgtttggcgc atcagaacaa tctgctctgg ctatcattcg ggaccataaa gacagcttca
     6361 gtgaaaaaga tattcagcct ctttatctgc gaaaaagtat tttactggta aagaaaggta
     6421 atcctaaaaa tatccggagt attgacgacc tgaccagacc cgggattggc gtaattgtta
     6481 atgatggtgg tggtaccagt aatacatcag gcactggcgt ctgggaagat attgccggac
     6541 gtaaagggaa tatagaaact gtcgccgcaa tccgaaaaaa tattatttta tatgcgccca
     6601 atagcggaac tgcacgtaag gctcttgaga atcagcctga agcagatgtc tggataacct
     6661 gggctgactg ggcagccagt aatccagaaa ttggtgatgt cgtggaaata gcgccagact
     6721 acgtgatatg gcgtgatatg aacattacag tacgtcagga tgcaaatgat gaaacccgtc
     6781 gatttgcaga atggctacaa accgatgaag cggcgcctgc attcaaaaaa tatggctgga
     6841 ccaggaaagg cacttgacat cctccacgtc cttcaggacg tggattcttt ttccggatgc
     6901 cgcgccagcg gcatgtaggg gcagctcaca aaacggaaaa aattgtacgc taagcctcgc
     6961 caggtgaact gaattcattc cgatatggga attcccatat cggacgaaaa cggcttgctg
     7021 taacggcaga gttaagtaga attgctgcgg gtgcttgagg ctatctgcct cgggcatgaa
     7081 caccaacggc agatagataa aagccccacc cgactataaa tcgaagtgag gcccctatat
     7141 gctcgtcaca tatagattgc ctcttacgga ccgaaaggtc aaggagaagc aggctatgaa
     7201 gcagcaaaag gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc
     7261 actggtaacg aggaaagacc tctgcgaggt acgaatccga accggccaga cggaggtcgc
     7321 tgtcttcaca gcttacgaac ctgaggagta agagaccagg cgggggagaa atccctcgcc
     7381 acctctgatg tgtcaggcat cctcaacgca cccgcactta acccgcttcg gcgggttttt
     7441 gtttttattt tcaacgcgtt tgaagttccg gacggcgccg gaatagaatc aaaaatactt
     7501 aagtagcgcg cagggagaag agggatggac cccgaacagg ggagtgctat ttatctggaa
     7561 ggattctgtt gatgaaaatc gaagaattac gtgaaatttt tagtgaagat ggcctctata
     7621 ctgtgcgcgt tgagaatggc gctattgtca gccactgccg tattaaatgt ttacagtctc
     7681 aacaaaggaa gagtggagct gcgttaattc attttgtgga tgggcttgtg acggatggtt
     7741 ttattttgcg tgcaaatgaa tttgtcacat cgttgccgtc tctgaaagac gctgggatta
     7801 aggctggttt ttctgctttt gaagatgagt gaattcatct acaattcagc gcagggctga
     7861 acccctgttg agtaacactg tgccaccgga gaaagccgat ggcgcaaaat tccagactac
     7921 acaattctga taattcagcc gtctttgcca gcaggcacgg gcggcgttct catgcattca
     7981 aatctgactg gttccggcac gacccatgca ctgaagaaca ggccgaatgg ctgattcaga
     8041 actaccgcag acgtgggtat gagtttagga aagccctcag cctcgattat cgtcactgga
     8101 taatctccgt caggcttcct tactccgaac gcccaccgcg tccgtcccgc acattccagc
     8161 aacggatctg gaggtaacgt gcgggtatta cttcgacctg ttctggtacc ggaactcggg
     8221 ctggtggtcc ttaagccagg tcgtgaatcc atgcaggtat ttcacaatac ccgggtactg
     8281 gtggagccgg aaccgaaaag catgcgtaat ctgccgtccg gggtcgttcc tgccgttcgc
     8341 cagccgctgg tggaagacaa aacattgctg ccatttttca gcgacgaacg agtgattcgt
     8401 gctgctggtg gtgctggtgc attgtctgac tggctgttgc gccatattaa atcctgccag
     8461 tggccacacg gcgattatca ccacagcgaa actgtcattc accgttatgg taccggccaa
     8521 tggtgttgtg ctggcactgc gacaaccagc tacgggacca gacatccgaa tcactcgagc
     8581 aacttgctca tcaaaacctg tcagcatgga tgattgacgt catcggtcac gcaataagcg
     8641 gtacgcagga gcgtgaatta tctctggctg aattatcctg gtgggcggtc tgcaatcagg
     8701 tggcggacgc gctaccggag gccgtattac gtcgttctct ggggttacgt gtggaaaaaa
     8761 tccgctcctt gtaccgcgaa agcgacatcg taccgggaga
//
LOCUS       DAZFYC010000170         8710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFYC010000170 DAZFYC010000000
VERSION     DAZFYC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.171"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..631
                     /locus_tag="ACHKW5_004682"
     CDS             <1..631
                     /locus_tag="ACHKW5_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO7513902.1"
                     /translation="NMKKTFSHCPYIKDPIIRSYIHSGEDNKFDDFMRQHRFSKVDFD
                     TQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVS
                     PLFIEIEDNLRTFNTNELTRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNS
                     TCIYKSRHKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            854..1351
                     /gene="ubiC"
                     /locus_tag="ACHKW5_004683"
     CDS             854..1351
                     /gene="ubiC"
                     /locus_tag="ACHKW5_004683"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO7513903.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1364..2236
                     /gene="ubiA"
                     /locus_tag="ACHKW5_004684"
     CDS             1364..2236
                     /gene="ubiA"
                     /locus_tag="ACHKW5_004684"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO7513904.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2391..4814)
                     /gene="plsB"
                     /locus_tag="ACHKW5_004685"
     CDS             complement(2391..4814)
                     /gene="plsB"
                     /locus_tag="ACHKW5_004685"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO7513905.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4985..5353
                     /gene="dgkA"
                     /locus_tag="ACHKW5_004686"
     CDS             4985..5353
                     /gene="dgkA"
                     /locus_tag="ACHKW5_004686"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO7513906.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5463..6071
                     /gene="lexA"
                     /locus_tag="ACHKW5_004687"
     CDS             5463..6071
                     /gene="lexA"
                     /locus_tag="ACHKW5_004687"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO7513907.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6144..7469
                     /gene="dinF"
                     /locus_tag="ACHKW5_004688"
     CDS             6144..7469
                     /gene="dinF"
                     /locus_tag="ACHKW5_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO7513908.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7585..7794
                     /gene="yjbJ"
                     /locus_tag="ACHKW5_004689"
     CDS             7585..7794
                     /gene="yjbJ"
                     /locus_tag="ACHKW5_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO7513909.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7836..8351)
                     /gene="zur"
                     /locus_tag="ACHKW5_004690"
     CDS             complement(7836..8351)
                     /gene="zur"
                     /locus_tag="ACHKW5_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO7513910.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2034 a         2076 c         2336 g         2264 t
ORIGIN      
        1 gaatatgaag aaaacattta gccattgtcc ttatataaaa gatccaatta tacgcagtta
       61 tatccattct ggtgaagata acaagttcga tgattttatg cgtcaacatc gattcagcaa
      121 ggtggatttc gatacccaac agatgatcca ttttattaac aggtttaata tgaataaagg
      181 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      241 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      301 tgtttcacct ctatttatag aaattgaaga taatttaaga acctttaaca cgaatgaatt
      361 aacacgaaag gataatatct ttatcctgtt ctcctctgcc gatgatgggc cagttatggt
      421 ggtaagctcc cagcgcttac atgatatgtt gaatcctaca aaagatacca attggaattc
      481 cacgtgtatc tataaatcca gacataagat gttgcctatt aatcttactc aggaaacact
      541 ttttagctcc aaatctcatg gtaaatatgc gcttttccca atttttactg cgagttggcg
      601 agctactcgt ataaagaata taggtattta agtaaaggaa aacatcaccg ttcctggcat
      661 catgggcggt gatgccccta cggttgccct cgccagcacg ggcatcggca aagcgtaagg
      721 ttcaacagcg gtttaccact tcatgcgatt gttgcgtttt tgttgcgcat tagatcactt
      781 aatttgcttt acatctcccg taaacacttt tctgcgatac aatgccttta cgttatgtaa
      841 cggagagttc ggcatgtcac accccgcgtt aacgcaactg cgtgcgctgc gctattttaa
      901 agagatccct gccctggatc cgcaactgct cgactggctg ttgctggagg attccatgac
      961 aaaacgtttt gaacagcagg gaaaaacggt aagcgtgacg atgatccgcg aagggtttgt
     1021 cgagcagaat gaaatccccg aagaactgcc gctgctgccg aaagagtctc gttactggtt
     1081 acgtgaaatt ttgttatgtg ccgatggtga accgtggctt gccggtcgta ccgtcgttcc
     1141 tgtgtcaacg ttaagcgggc cggagctggc gttacaaaaa ttgggtaaaa cgccgttagg
     1201 acgctatctg ttcacatcat cgacattaac ccgggacttt attgagatag gccgtgatgc
     1261 cggactgtgg gggcgacgtt cccgcctgcg attaagcggt aaaccgctgt tgctaacaga
     1321 actgttttta ccggcgtcac cgttgtacta agaggaaaaa aatatggagt ggagtctgac
     1381 gcagaataag ctgctggcgt ttcatcgctt aatgcgtacg gataagccaa ttggcgcgtt
     1441 actgctgctc tggccaacat tatgggcgtt gtgggtggcg acaccgggcg ttcctcagct
     1501 ctggatcctg gcggtatttg tcgcgggtgt ctggctgatg cgcgctgctg gatgtgtggt
     1561 gaatgattat gctgaccgaa agtttgatgg tcatgttaag cgcacggcga accgaccact
     1621 tcccagcggc gcggtaacag agaaagaggc gcgcgcactg tttgtagtgc tggtactgat
     1681 ttcgttttta ctggtactga cgctgaatac gatgaccatt ctgttgtcga ttgccgctct
     1741 ggcgttggcg tgggtgtacc cgtttatgaa gcggtatacc catctcccgc aagtggtgct
     1801 gggcgcggcg tttggctggt cgattccaat ggcttttgcc gctgtgagtg agtcggtgcc
     1861 attgagttgc tggttaatgt tcctcgccaa tattctctgg gcggtggctt acgacacgca
     1921 gtatgcgatg gttgaccgcg atgatgatgt gaagattggc attaaatcca cggcaatcct
     1981 gttcggccaa tacgataaat tgattattgg tattttgcag attggcgtac tggcactgat
     2041 ggcgatcatc ggtgagttaa atggcttagg ctggggatat tactggtcaa ttgtggtggc
     2101 tggcgcgctg tttgtttatc aacaaaaact gattgccaac cgcgagcgtg aagcctgctt
     2161 taaagcattt atgaataata actatgttgg tctggtacta tttttagggc tggcaatgag
     2221 ttactggcat ttctgatgat gtaaaaaagc cggatgatca tccggctttc ttctgggttg
     2281 cctgatgcgc ggcgcttctc aggcctacac aacacatcgc aatttattga atttgcagat
     2341 tatggaaggc cggataaggc gttttcgccg catccggcaa ttctctctga ttacccttcg
     2401 ccctgcgtcg cactctcaat cgtcaaacgc acgtctgatg taatcaactc cgccagcaac
     2461 tgataaacct tcatcgtttc tgccggttcg gcgtcgccgc tatcgctgat atacccttca
     2521 tcacgcagcg tcagcaccag agaactgaac accgccttgt cgaagaactc cggcgcgttg
     2581 atgccgtgca gcacggagag acgttgcgcg acggtgcggc tctctttctc cagcgtaccg
     2641 cggttgatcg acgggttggc actcaacaac cagaaggtga tggcataacg ttgcagcgtt
     2701 tcgcgcgcgc ctgcggccag cagctgtagg gtgcgagaat gcgccgggtt gatatgcaac
     2761 tcatcatctt gcagggtaat cagcccctga cgttgcatct catttgccag cgcatcaata
     2821 acgtccggca actcgtcgcg atcccagcgc aggaacagct ccgctttcag cattgggtaa
     2881 agcacattga cgtgctccat caatacgtcg cgggagatgt ggcgatgctg ggtgacaatt
     2941 gccgccatca gcgaaggcag caccaacata tgcgcaatgt tgttgcgata gtaggtcatc
     3001 agcaccgctt gctcgcgtgg cagaatgatg atgtcgccga tagtgtcttt ctcgacttca
     3061 aacttgttca tttgcagcgc gtgatcgata agctcgctgg cgctggctga aggaacggta
     3121 gagtccgtgg agtaaggtac gttgcgcatc agatccaggt agcagttgag ttgctcggtt
     3181 aactgctcgc gggtgagtga gcgctgacgt gatgccagta gcgcagtaca gcacaggttc
     3241 atggcgtttg ccgcgcctgc gttgttaatg cgtaccatca gatcggcagc aatattattg
     3301 accgtcggcg ttaaccaggc cggacgcacc gcttcgatgg gatcgataga ttcacgccag
     3361 tctggtacgt gctggttaag gtaggtcatc aacggcattg gttcaccgaa gttgacgtaa
     3421 ccctgaccga gattacgcag cttgcttaaa ccgcgcagca tctgcggcag gctctctttc
     3481 tctttcgtcg cgccgcgcag ttctttggcg taagtaccca cttccatgac gtgctcataa
     3541 ccgatataga tcggaatcag cgtaatcgga cgcgtgccgc cacgcagcat cgcctgaatg
     3601 gtcatcgaca gcgtaccggt tttcggatcc agcaaacgcc ccgtgcggga acgaccgcct
     3661 tccacgaagt actcgacgga ataaccacgg ctgaacagtt cgccgagata ctcacggaaa
     3721 acggtggaat aaagtttatt gcctttaaaa gtacggcgaa taaagaacgc ccccagacgg
     3781 cggaaaatcg gcccggccgg ccagaagttc aggttgatcc cggcggcgat atgcggcggc
     3841 accagcccct ggtggtacag cacgtaagaa agtagcaggt agtccatgtg actgcggtgg
     3901 caaggcacat acaccagctc atgaccgtcg tgggccagct ggcgaacgcg ctcggcgtta
     3961 tggacgttga tgccctgata aagtcggttc caggtgaagc ccagaatgcg gtcagtcagg
     4021 cggatcatct cgtaagagaa attcgccgca atctcttcca tcagcgcaat cgcgttctgc
     4081 tgcgcttttt catgggagat ttttttgctg cgcgcttcat cttctaccgc tttggcaatg
     4141 gcgcgggagg cgagcagctt attaaacaga tcctgacgag caggcagacg tgggcccaca
     4201 gcagccagac gttgacgggc aaagtgcata cgcgccacgc gcgccagttt ctgtgcgata
     4261 gttttatccg tgccgtgttc atccgccata cggcgcagcg aaactgacgg cgagaaacgc
     4321 acaaaactgt cgcgaccgag ccacagtacg gcgaaaaatt tctgtacgcc gttaagcata
     4381 cgcagcggcg ggtttacttc gcctttttca cgccccggcg cgcgaccaaa catcaccgat
     4441 actggcacca tctgcacatc cagatttggg ttgctacggt gcaaatcgag atagtcgtgg
     4501 aacagcttaa tagactcttc tttcggcgtg taataggtga atacacgcgg cccgccgtga
     4561 atgaacacat agcgcggcag tagcgtgccg tcgatttcca gcggctctaa cgggtcaggc
     4621 aagtcatgag ccagacactg ggcgcgtaac gtcagcaaat ctgctttcga gttgtacggt
     4681 aaaacgtaca taattggacg agaagtatcc agccccagtt ccggggcagg atctgccgga
     4741 atagacttgc tttttaccag gatgcttaat ggtaaattca gtaatttgta gtaaattcgt
     4801 ggccagccgg acataaacga tgtaaagcct ctggttaata atgcaaatgc gcggcaagga
     4861 tagcagaaag tcatgggaaa ttctgtggta tccgctcatg tttcgcgcgg cgttacgcaa
     4921 acccgaatca tcggatttaa cggtacactg atattgacgc tcataatgta aaaaggttct
     4981 ttcaatggcc aataatacca ctggattcac ccgaattatc aaagctgctg gctattcctg
     5041 gaaaggttta cgcgctgcat ggatcaacga agcggcattc cgtcaggaag gcgtagcggt
     5101 attgttggcg gtggtcatcg cctgctggct ggatgtggac gcaattaccc gcgtgctgct
     5161 tatcagctcc gtgatgctgg tgatgattgt ggaaattctc aatagcgcca tcgaagcagt
     5221 ggttgaccga attggctctg aataccatga gctttccgga cgcgctaaag atatgggatc
     5281 cgccgcggtg ctgattgcca ttatcgtcgc cgtgattacc tggtgcattc tgttatggtc
     5341 gcattttgga taacccttcc agatttcgat aaatctctgg tttattgtgc agtttatggt
     5401 tccaaaatcg ccttttgctg tatatactca cagcataact gtatatacac ccagggggcg
     5461 gaatgaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt gatcacatca
     5521 gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc
     5581 caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt
     5641 ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg ccgctggtag
     5701 gtcgtgtggc tgccggtgaa ccgcttctgg cgcaacagca tattgaaggt cattatcagg
     5761 tcgatccttc cttgttcaag ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga
     5821 tgaaagatat cggcattatg gatggtgact tgctggcagt gcataaaact caggatgtac
     5881 gtaacggtca ggtcgttgtc gcacgtattg atgacgaagt taccgttaag cgcctgaaaa
     5941 aacagggcaa taaagtcgaa ctgttgccag aaaatagcga gtttaaacca attgtcgttg
     6001 accttcgtca gcagagcttc accattgaag ggctggcggt tggggttatt cgcaacggcg
     6061 actggctgta acatatctct gagaccgcga tgccgccagg cgtcgcggtt tgtttttcat
     6121 ctctcttcat caggcttgtc tgcatggcat tcctcacttc atctgataaa gcactctggc
     6181 atctcgcctt acccatgatt ttctccaata tcaccgttcc gttgctggga ctggtcgata
     6241 cggcggtaat tggtcatctt gatagcccgg tttatttggg cggcgtggcg gttggtgcaa
     6301 cggcgaccag ctttctcttt atgctgttgc tgtttttacg catgagcacc accgggctga
     6361 ctgcgcaggc ttatggtgcc aaaaatcctc aggcattagc ccgtgcgctg gtgcaaccgt
     6421 tgctgttggc gttgggggct ggggcgttaa ttgcgctgct gcgtacaccg attatcgatc
     6481 tggcgctgca tattgttggc ggtagtgaag cggtcctgga acaggcgcgg cgctttcttg
     6541 aaatccgctg gttaagcgct ccggcgtcgc tggcgaatct ggtattactc ggttggttac
     6601 tcggcgtgca atatgcccgt gcgccagtaa ttttgttagt ggtcggcaat atcctcaaca
     6661 ttgtgctgga tgtctggctg gtgatggggc tgcatatgaa cgtgcagggc gcggcgctgg
     6721 cgacggttat tgcggaatat gcaacattgc tgattggtct gctaatggtg cgtaaaatcc
     6781 tcaaactacg cggaatttcc ggcgaaatgc tgaaaactgc ctggcgagga aacttccgtc
     6841 gcttgctggc gcttaaccgc gatatcatgc tgcgttcgct gttgttgcaa ctctgtttcg
     6901 gcgcgatcac cgtacttggt gcgcgactgg ggagtgacat tgtcgctgtt aacgcggttc
     6961 tgatgacgct actcaccttt accgcctatg cgcttgatgg ttttgcctac gcggttgaag
     7021 cgcactccgg tcaggcatac ggtgcgcgcg acggtagcca gttgctggat gtatggcggg
     7081 cagcgtgccg ccagtcgggg attgtagcgt tactgttttc ggtggtttat ttgctggcag
     7141 gggaacacat cattgcgttg ctgacgtcgt taacccagat tcagcagctg gctgaccgct
     7201 atcttatctg gcaggtgatt ttgccgttgg ttggcgtctg gtgttatctg ctggacggca
     7261 tgtttatagg cgcaacgcgt gccgccgaaa tgcgtaacag tatggcggtg gccgccgcag
     7321 gttttgcgct gacgctcctt acgctgccgt ggctgggtaa tcatggtttg tggctggcat
     7381 taaccgtctt tctggcgttg cgcgggcttt ctctggcggc tatctggcgg cgtcactggc
     7441 gcaacggtac ctggtttgcc gcaacgtgac ggttaaaaat tctgaataaa ttatcctaag
     7501 ccaaattgct gactacactt aatctcacgt tcagaagaaa agtgaacgta ctctcattca
     7561 caacctaacg atgaggtctt gattatgaat aaagatgaag ccggcggtaa ctggaaacag
     7621 tttaaaggta aagtgaaaga gcaatggggc aaactgaccg atgatgatat gacgatcatt
     7681 gaaggtaaac gtgatcaact ggtcggtaaa atccaggaac gttatggtta tcagaaagat
     7741 caggcagaaa aagaggtcgt ggattgggaa acccgcaatg aatatcgctg gtaattaatc
     7801 cctcctgccc gacgtgtaca aggatgtacg ccctcttaac gcggtttctt tttcacctgc
     7861 acagagtgat catgctggca ctgttcagga tgacgacacg cttccacttc cacgcatgcc
     7921 gcacagagcc catgtgcttc aatcacatta tgccgcaggg caaaccccat ttttgccgcc
     7981 agcgtatgca taatgtcttc cacgccttct gcacactctt ctttcaccgc gccgcagcga
     8041 tcgcatataa acatggctga cgtatgcgtg ggctggtcga acagatgaca gagcacataa
     8101 ctgttggtgg attccacctt atgcacaaaa ccttgctcaa gcagaaaatc cagcgcgcga
     8161 taaaccgttg gcggcttggc ttgcggttca gcttcgcgca gtaaatcaag cagatcataa
     8221 gcgctgatag cgccatcctg cagactcatc aggcgcaaca cttccaggcg ctgtggggtc
     8281 aggcgcacat tacgctgcgc gcagattttt tcagcctgcg ctaataactc ctgcgttgtg
     8341 gtcttttcca tcgggcacct caaagtgggg gttaagaatg tccttacttt accatgttcc
     8401 aggaaaaaca ccgagaaccc ttattgttgc cgtaatgttg attttctgtt ttgtaggtaa
     8461 ggtgttgtgt tgccttgtcg taccattatc aacacgataa taattaataa ttcattttta
     8521 aataaattgc ttcaagttaa ctcgctattt atttaactgg gtttaaacgc atacctgtag
     8581 ttcagaaatt atgagcgatt tttgatagtg accagatggc aaaagaatta taaggaggac
     8641 acggtacgac aaaaaaggta gatgaatcat gttgaagata atacccggtg caactggata
     8701 ctttaataaa
//
LOCUS       DAZFYC010000171         8583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFYC010000171 DAZFYC010000000
VERSION     DAZFYC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.172"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(302..508)
                     /locus_tag="ACHKW5_004691"
     CDS             complement(302..508)
                     /locus_tag="ACHKW5_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO7513911.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            821..910
                     /gene="kdpF"
                     /locus_tag="ACHKW5_004692"
     CDS             821..910
                     /gene="kdpF"
                     /locus_tag="ACHKW5_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO7513912.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            910..2583
                     /gene="kdpA"
                     /locus_tag="ACHKW5_004693"
     CDS             910..2583
                     /gene="kdpA"
                     /locus_tag="ACHKW5_004693"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO7513913.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2606..4654
                     /gene="kdpB"
                     /locus_tag="ACHKW5_004694"
     CDS             2606..4654
                     /gene="kdpB"
                     /locus_tag="ACHKW5_004694"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO7513914.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4663..5235
                     /gene="kdpC"
                     /locus_tag="ACHKW5_004695"
     CDS             4663..5235
                     /gene="kdpC"
                     /locus_tag="ACHKW5_004695"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO7513915.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5228..7912
                     /gene="kdpD"
                     /locus_tag="ACHKW5_004696"
     CDS             5228..7912
                     /gene="kdpD"
                     /locus_tag="ACHKW5_004696"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO7513916.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRTAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSYDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7909..>8583
                     /gene="kdpE"
                     /locus_tag="ACHKW5_004697"
     CDS             7909..>8583
                     /gene="kdpE"
                     /locus_tag="ACHKW5_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO7513917.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1796 a         2223 c         2486 g         2078 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgctgacag gcgaaaccct acagatttat
      121 aagaaagcct taagtgagat atctgtcaga taagaacaat taggaaaata tttatatata
      181 tttcatgaat atatgtttcg attggtataa cgatgtctaa atattcctct gaacatcttc
      241 ctgatacaaa aaaccgccag cgtttccgct ggcggttttc aatgtattca aaactcagca
      301 atcaataaaa atcaccagtt gccttttcgg cctgatccat ccacaccggt ttatcgctgg
      361 ttttcgacca gacgcgatgc aggtagctgt aaaagcgggc gcggtctttc cagaaaagca
      421 tgaaaggcag cgccagaacg cccgctgcaa ccgcataagt acggcgtaaa aagataagcc
      481 atgcaggata ttctctgtag agttccatac ttttctcccc cttttgtatc tacccggtga
      541 atggcaccgg aaaaatgaat ttgtttatct gatgaaaata gtaccgcctt ttgtgtaatt
      601 ttactactca tccgaccact tatttttgct tattgatggt ttatttacat tcatcctgta
      661 attaagttac acaaaagtta aattaatact aaacattagt taaatcatgg cttttgccat
      721 ttttatactt tttttacacc ccgcccgcag atttttgcga aatctttgca gccagaattc
      781 tacccttccg gtatcacttt taggccactg gaggtgcact gtgagtgcag gcgtgataac
      841 cggcgtattg ctggtgtttt tattactggg ttatctggtt tatgccctga tcaatgcgga
      901 ggcgttctga tggctgcgca agggttctta ctgatcgcca cgtttttact ggtgttaatg
      961 gtgctggcgc gtcctttagg cagcgggctg gcgcggctga ttaatgacat tcctcttcca
     1021 ggtacagcgg gcgttgaacg tatactcttt cgtttacctg gcgtctctga ccatgagatg
     1081 aactggaagc aatatctttg tgccattctc ggcctgaaca tgctggggct ggcggtgctg
     1141 ttttttatgt tgctcggtca gcactatctg ccgcttaatc cacagcagtt gccggggctg
     1201 tcgtgggatc tggcgctgaa taccgccgtc agctttgtca cgaataccaa ctggcaatct
     1261 tacagcggtg aaaccacgtt gagctatttc agccagatgg ctggcttaac ggtgcaaaac
     1321 tttctttctg ccgccagcgg gattgcggtg atttttgccc tcatccgtgc gtttacccgc
     1381 cagagcatga gcacactcgg gaatgcctgg gtcgatctgc tacgcatcac gttatgggtg
     1441 ctcgtccctg tggcgttgtt gatcgcactg ttttttattc aacaaggtgc gctgcaaaac
     1501 tttcagcctt atcaggctgt gaataccgtt gaaggagcgc aacagctgtt acccatgggg
     1561 cctgtagctt ctcaggaagc gatcaagatg ctcggtacta acggcggtgg cttctttaat
     1621 gccaactcgt cgcatccgtt tgaaaaccca accgcactga ccaacttcgt gcagatgctg
     1681 gcgatcttct tgatcccaac ggcgctgtgc tttgcctttg gtgaagtgac gggcgatcgc
     1741 cgccaggggc gcatgttgct gtgggcgatg tcagtgattt ttgtcatctg cgtaggcgtg
     1801 gtgatgtggg cagaagttca gggtaatcct catctgctgg cgctcggcgc agacagcagc
     1861 atcaatatgg aaggtaaaga gagccgtttc ggcgtgctgg tcagtagcct gtttgcggtc
     1921 gtgactacgg cggcttcctg tggcgcggtg attgcgatgc atgattcgtt taccgctctc
     1981 ggtggcatgg tgccgatgtg gctgatgcaa attggtgaag tggtgttcgg cggtgtcggt
     2041 tctggtcttt acggcatgat gctgtttgtc ctgctggcgg tgtttattgc cgggctgatg
     2101 attggtcgta caccggaata tctgggtaaa aaaatcgacg tacgcgagat gaaactgact
     2161 gcactggcga ttctggttac cccgacgctg gtgctgatgg gcgcggcgct ggcaatgatg
     2221 accgacgccg gacgtagcgc catgctcaac cctggcccgc atggttttag cgaagtgctg
     2281 tacgccgtgt catccgccgc taacaacaac ggcagcgcct ttgccggatt aagcgccaac
     2341 tccccgttct ggaactgttt actggcgttc tgcatgtttg tcggtcgctt cggggtgatt
     2401 atcccggtga tggcaattgc cggttcgctg gtgagtaaaa agagccaacc cgccagctcc
     2461 ggcacgctgc caacgcacgg cccgctgttt gttggcctgt taatcggcac cgtgttgctg
     2521 gttggcgcac tgacctttat ccctgccctg gcgcttggtc cggtggcgga atatctctcc
     2581 tgatgatatt gagtgagcac tgaatatgag tcgtaaacaa ctggcgctat tcgaaccaac
     2641 acttgtcgtt caggcgctga aagaagcggt gaaaaaatta aacccgcagg cgcaatggcg
     2701 caatccggtg atgtttatcg tctggatcgg cagtctgctg accacctgta ttagcatcgc
     2761 gatggcaagc ggtgcgatgc ctggcaatgc gctgtttagc gcggcaatta gcggctggct
     2821 gtgggtcacc gtactgttcg ctaatttcgc cgaggcgctg gcagaaggcc gcagtaaagc
     2881 gcaggccaac agtcttaaag gggtgaaaaa aactgccttt gcccgcaagc tgcgtgagcc
     2941 gaaatatggc gctgcggcgg acaaagttcc tgccgatcaa ctgcgtaaag gtgacatcgt
     3001 gctggtagaa gctggcgata ttatcccctg cgatggtgaa gtcattgaag gcggcgcatc
     3061 ggtcgatgaa agcgccatca ccggggaatc ggcaccggtg atccgtgaat ccggcggcga
     3121 ttttgcctcc gtcaccggcg gcacgcgtat tctttctgac tggctggtga ttgagtgtag
     3181 cgttaacccc ggtgagacat ttctggatcg gatgatcgcg atggtggaag gcgcacagcg
     3241 acgcaaaaca ccgaacgaga ttgccctgac tatcctgctg attgctctga ccatcgtctt
     3301 tttactggca accgccacgc tgtggccgtt ttccgcgtgg ggcggtaatg ccgtgagtgt
     3361 aacggtactg gtggcgctgc tggtctgtct gatcccaacc actattggcg gcctgttgtc
     3421 agcgatcggc gtcgccggga tgagtcggat gctgggcgcg aatgtgattg ccaccagcgg
     3481 acgtgcagtt gaagcggcag gtgacgttga cgttctgcta ctggataaaa ccggcaccat
     3541 cacactcggt aaccgtcagg cgtcggagtt tatccctgcg cagggcgtgg atgaaaaaac
     3601 gctggctgac gccgcacaac tggcttcgct ggctgatgaa acgccggaag gccgcagtat
     3661 tgtgatcctc gccaagcaac gttttaacct gcgcgagcgc gatgtgcagt cgctccatgc
     3721 cacctttgta ccgtttactg cgcaaagccg gatgagcggg atcaacatcg acaaccgcat
     3781 gatccgtaaa ggttctgtcg atgccattcg ccgccatgtt gaggctaacg gcggtcactt
     3841 ccctaccgat gttgatcaaa aagtcgatca ggttgcgcgt cagggggcca cgccgctggt
     3901 ggtggtggaa ggttcccgtg tgctgggcgt tattgcgctg aaagatatcg tcaaaggcgg
     3961 tattaaagag cgcttcgccc agctgcgcaa aatgggcatt aaaacggtga tgattaccgg
     4021 cgataaccgt ctgactgccg ccgccattgc tgcggaagcg ggcgtcgatg attttctcgc
     4081 cgaagcgaca ccggaggcca agctggcatt gattcgtcag tatcaggcgg aaggccgttt
     4141 ggtggcgatg accggcgacg gcaccaacga tgctccagcg ctggcgcagg cagatgtcgc
     4201 ggtggcgatg aactccggta ctcaggcggc gaaagaagcg ggcaatatgg tcgatctcga
     4261 ctctaacccg accaagttga tcgaggtggt acatattggc aaacagatgc tgatgacccg
     4321 tggctcgctg accaccttca gcattgccaa cgatgtggcg aaatacttcg ccattattcc
     4381 ggcggcgttc gcggcaacgt atccgcagtt aaatgcgctg aacatcatgc gccttcattc
     4441 gcccgactcc gccattctca gtgcggtcat tttcaacgcc ttgattatcg tctttttgat
     4501 tcctctggcg ttaaaaggcg tgagttataa accgcttacc gcttctgcca tgttgcgccg
     4561 taacttatgg atttacggtc tgggtgggct gctggtgccg tttattggta tcaaagtcat
     4621 tgatttactg ctgaccgttt gcggtctggt gtgaggttta ccatgcgtgg cttacgtccg
     4681 gcattatcga catttatctt tctgttattg attactggcg gcgtttaccc gctgctgacc
     4741 accgtgctgg ggcaatggtg gtttccctgg caggccaacg gttcgttgat tcgtgaaggt
     4801 gatacggtgc ggggttcggc attaatcggg cagaatttta ccggcaacgg ctattttcag
     4861 ggtcgcccgt cggcaacggc agaaatgccc tataatccac aggcttctgg cgggagcaat
     4921 ctggcggtca gtaaccctga gctggataaa caaatagccg cacgcgttgc tgcattacgg
     4981 gccgctaacc cggatgccag tacgaacgtt ccggttgaac tggtgacggc atcggcaagc
     5041 gggctggaca ataatatcac cccgcaagcg gcggtctggc aaatcccacg cgtggcgaaa
     5101 gcgcgtaatc tcagcgttga acagctcacg caactgatcg caaaatacag ccaacaaccg
     5161 ctggtgaaat atatcggcca gccggtcgtc aacattgttg aactcaatct ggcgctggat
     5221 aaacttgatg aataacgaac ccttacgtcc cgaccccgat cgtctgctgg aacaaactgc
     5281 cgcgccgcat cgggggaagc tgaaagtttt cttcggtgcc tgtgcaggcg tcgggaagac
     5341 ctgggcgatg ctggcagaag cccagcgact gcgggcgcaa gggctggata ttgtggttgg
     5401 cgtggtagaa acccatgggc ggaaagatac cgccgccatg cttgaagggc tggctgttct
     5461 gccgcccaaa cgccaggcgt accgtgggcg gcatatcagc gagtttgatc tcgatgccgc
     5521 cctcgcccgc cgtccggcgc tgatcttaat ggacgaactg gcgcacagta atgcgccagg
     5581 ttctcgtcat cctaaacgct ggcaggatat cgaagaactg ctggaagctg gcattgatgt
     5641 tttcacaacc gtcaacgttc agcatctgga aagtctgaat gatgtggtca gcggcgtcac
     5701 cggaattcag gtacgggaaa ccgtgcccga tccttttttc gatgccgccg acgacgtagt
     5761 gctggtggac ttgcccccgg acgatctgcg ccagcggctg aaagaaggca aagtctatat
     5821 tgccgggcag gcggagcgcg ccattgaaca ttttttccgc aaaggcaatc tgatcgccct
     5881 gcgtgaactg gcactgcgcc gtactgccga tcgcgtagat gagcaaatgc gcgcctggcg
     5941 ggggcatcct ggtgaagaga aagtgtggca cacgcgcgac gcgatccttt tatgcatcgg
     6001 gcataacacc ggcagtgaga aactggtccg cacggcggcg cggttggcct cgcgcctggg
     6061 tagcgtctgg cacgcggtgt atgttgaaac ccctgccctg catcgcttac cggaaaaaaa
     6121 acgtcgggca attctcagcg ccttacgtct ggcgcaggag ctgggcgcgg agacggctac
     6181 actttctgat ccagcggaag aaaaagcagt agtgcgttat gcccgtgaac ataatctcgg
     6241 caagattatt ctcggtcgcc cggcctcgcg ccgctggtgg cgtcgggaaa cgtttgctga
     6301 ccgactggcg cgcatcgccc ccgatctcga tcaggtgctg gtcgcgcttg atgaaccacc
     6361 cgcccgcacg attaacaacg cgccggatag ccgctctttt aaagacaagt ggcgggtaca
     6421 gattcaggga tgcgtggttg ccgccgcgtt atgcgccgtt atcactttaa ttgccatgca
     6481 gtggctgatg gcgtttgatg ccgccaacct ggtgatgctg tatctgcttg gcgtggtggt
     6541 ggtggcgcta ttttatggac gctggccttc ggtggttgct accgtcatta atgtggtgag
     6601 ttacgatctc ttttttatcg ccccacgcgg cacgctcgct gtctctgatg tgcaatatct
     6661 gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc gggaacctta ctgcgggtgt
     6721 gcgttatcag gcgcgggtag cccgttaccg cgagcaacgc acacggcact tatatgaaat
     6781 gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc gccgccacca gcgaacaatt
     6841 tattgcctcc acgtttcatg cccgcagtca ggtgttgttg cccgatgaca acggtaaatt
     6901 gcagccgtta acacatccgc aaggaatgac gccatgggac gatgccatcg cgcagtggag
     6961 ttttgataaa ggcctgcctg cgggtgcggg caccgacacg ttacccggcg tgccgtacca
     7021 gattttgccg ctaaaaagcg gcgagaaaac ctacggactg gtggtggtgg aaccggggaa
     7081 tctgcgtcag ttgatgatcc cggagcagca gcgcctgcta gagacgttta cgctgttagt
     7141 cgccaatgcg tttgagcggc tgacgctgac cgccagtgaa gaacaggcgc ggatggcaag
     7201 cgaacgtgaa cagatccgca acgccctgct ggcggcgctt tcgcatgatt tacgcacgcc
     7261 gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa gcgaaggatc
     7321 gccccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta cccgactggt
     7381 gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga agaaagagtg
     7441 gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ctggaaccgg gtttatcgtc
     7501 gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg ggccactctt
     7561 tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg cgcaggccga
     7621 aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct gggataacgg
     7681 ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc gcgggaataa
     7741 agagtcggca gtaccggggg tagggcttgg actggcaatt tgtcgggcga tagtggatgt
     7801 acacgggggc actattaccg cgttcaaccg accggaaggt ggtgcctgtt ttcgtgttac
     7861 acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt gacaaacgtt
     7921 ctgattgttg aagatgaaca ggctattcgt cgctttctgc gcacggcgct ggagggcgac
     7981 gggatgcgcg tctatgaggc cgaaacgctg caacgcggct tgctggaagc ggctacccgt
     8041 aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat tgagtttatc
     8101 cgcgacctgc gccagtggag cgcggtgccg gtgattgtgc tttccgcacg cagcgaagag
     8161 agcgacaaaa tcgccgcgct ggatgccgga gcggatgatt atctgagtaa gccgtttggc
     8221 attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc caccgccgcg
     8281 cccgatccgc tggtgaaatt ttccgatgtt accgtcgatt tagccgcccg cgtgattcac
     8341 cggggtgagg aagaggtgca tctcacaccg attgagttcc gcctgctggc ggtgctgctg
     8401 aataatgccg ggaaagtgct cacccagcgc cagttactca atcaggtgtg ggggccaaac
     8461 gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca aaaactggaa
     8521 caggatcccg ctcgcccccg ccatttcatt actgaaaccg gtattgggta tcggtttatg
     8581 cca
//
LOCUS       DAZFYC010000172         8390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFYC010000172 DAZFYC010000000
VERSION     DAZFYC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.173"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..308
                     /locus_tag="ACHKW5_004698"
     CDS             <1..308
                     /locus_tag="ACHKW5_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513918.1"
                     /translation="VFHAFIDLVNKIRGKKDFMYICTLCCFAEEVKGDYSHYRTFLFD
                     IGNQYKVKLTQSGKKELSLTLEFNDTIIESQKVTGNKAKHILEDIEKFYRNKPDTYY"
     gene            complement(530..1723)
                     /gene="tyrB"
                     /locus_tag="ACHKW5_004699"
     CDS             complement(530..1723)
                     /gene="tyrB"
                     /locus_tag="ACHKW5_004699"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO7513919.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1976..3055)
                     /gene="alr"
                     /locus_tag="ACHKW5_004700"
     CDS             complement(1976..3055)
                     /gene="alr"
                     /locus_tag="ACHKW5_004700"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO7513920.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGIIKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(3108..4523)
                     /gene="dnaB"
                     /locus_tag="ACHKW5_004701"
     CDS             complement(3108..4523)
                     /gene="dnaB"
                     /locus_tag="ACHKW5_004701"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO7513921.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4606..5589
                     /gene="qorA"
                     /locus_tag="ACHKW5_004702"
     CDS             4606..5589
                     /gene="qorA"
                     /locus_tag="ACHKW5_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO7513922.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5755..5997)
                     /gene="pspG"
                     /locus_tag="ACHKW5_004703"
     CDS             complement(5755..5997)
                     /gene="pspG"
                     /locus_tag="ACHKW5_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO7513923.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(6131..7168)
                     /gene="dusA"
                     /locus_tag="ACHKW5_004704"
     CDS             complement(6131..7168)
                     /gene="dusA"
                     /locus_tag="ACHKW5_004704"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO7513924.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7531..8238)
                     /gene="yjbM"
                     /locus_tag="ACHKW5_004705"
     CDS             complement(7531..8238)
                     /gene="yjbM"
                     /locus_tag="ACHKW5_004705"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO7513925.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(8262..>8390)
                     /gene="yjbL"
                     /locus_tag="ACHKW5_004706"
     CDS             complement(8262..>8390)
                     /gene="yjbL"
                     /locus_tag="ACHKW5_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO7513926.1"
                     /translation="NIYGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         2225 a         2124 c         1950 g         2091 t
ORIGIN      
        1 aagtttttca cgcatttatt gatttagtta acaaaatcag gggtaagaaa gactttatgt
       61 atatttgcac gctttgctgt tttgccgagg aggttaaagg tgattattct cattacagga
      121 cctttttatt tgatattggt aatcaatata aggttaaact tacacaaagc ggaaaaaaag
      181 agctctcttt aactttagaa tttaacgata ctataattga atctcagaaa gtcacaggca
      241 ataaggcaaa gcatattctt gaggatatag aaaaattcta tcgtaacaaa cccgatactt
      301 attattaaat ttaataatag ttagatgtgc agaaattatt actccaccca aaaaaacagg
      361 ataaggaatt ccagataatt aggataagct taacaataaa agaactaata acgtttatta
      421 tctctcacgt agaacgatgg catcaaaaaa atcatctata aagaaagagt gaaagcgacg
      481 acagtttaat ttcactgcag gctgggtagc tccagcctgc tttcctgcat tacatcaccg
      541 cagcaaacgc ctttgccaca cgttgtacat ttgccgtatt taacccggcg acacacatgc
      601 gaccgctggc gatgagatag acaccaaatt cttcacgtag tcggtcaacc tgagcggcac
      661 ttaaaccggt ataactgaac atgccgcgct gattaagcag ataatcgaaa ttgcgttctg
      721 gcatctctgt gcttaatacc ttcaccaatt cctgacgcat tgccagaatg cgagtacgca
      781 tctcttctac ttccgccagc cagctggctt tcaatgcctc gtcattcagc actgcagcca
      841 ccacctgcgc accaaaattc ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt
      901 gccccagtac gcggccagcg gcttcggcat cttcacacag aacagaaagt ccgccgacgc
      961 gctcgccgta aagggagaaa attttcgaga acgaattgct caccagagcg ggtaatccag
     1021 cgctggcaat ggcgcgaata gcgtaggcat cctcttccat accggcacca aatccttgat
     1081 aggcaatatc gaggaatgga ataagctcgc gggctttgag aatttcaatc accgcatccc
     1141 actgatcatt agtgagatcg gcacccgttg ggttgtggca acatggatgc agcaacacaa
     1201 tactgcgggc aggtaatgtt ttcagcgtcg ccaacaggtc attaaagcgc acgccgttag
     1261 tcgcttcgtc ataccagggg taagtactca cttcgaatcc agccccggcg aatattgcta
     1321 cgtggttttc ccaggtagga tcgctgaccc agacgcctga ttccgggaag tagcgtttca
     1381 ggaaatccgc gcccactttc aatgcccctg agccgccaag ggtttgaatg gttgctacgc
     1441 gctgttgttg cagtactgga tggtccgcac caaacagcag cggcgcaatg gcatggcgat
     1501 agctgttaag cccttccatc ggtaaataaa gcgaagcgcc atgaggctgc gcattcaggc
     1561 gcgcttccgc ctccgccacg gcttgcagtt gtggaataat tccgtcttcg ttgtagtaca
     1621 gaccgatact taaattcact ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg
     1681 taagaatcgg gtcgccagcg taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt
     1741 ttacgggcag gtggttaaaa cacaataaac cggaagaagg cgaagatcga gtggatgttc
     1801 aggagcgaac ggcaattagc aacagagtga gactcatgac aaacgtacat ccgccagagg
     1861 cacgaccttt ataagagcat gaaagaacat caacttattg aatttttagg attttattgg
     1921 ccggataagg cattcacgcc gcatccggca cagacaatca aatattacag aacgattaat
     1981 ccacgtattt catcgcgacc cttgaagtca ggcgcgtaat aagttcgtaa gcgcttactt
     2041 tcgtcatttc agcgatacgt tctacgggca aaccttcgcc ccataaaatg accggatccc
     2101 cggctttgtc ctgcgcttgt ggacctaagt ctacgcagat catatccatc gccacgcgcc
     2161 cgacaatcgg tacttcgcga ccgttcacca gcactggcgt accggacggc gcggcgcgcg
     2221 gataaccatc gccatagccc atcgcgacta cgccaagacg ggtatcacgt tcgcttaccc
     2281 aggttccacc ataaccaaca ggttctccgg ctttatgctc acgcacggca atcaggctgg
     2341 aggttagtga catcactggc tgacagccaa aatcggcacc ggtggagcga tcttccagcg
     2401 gcgagacgcc ataaagaatg atgcccgggc gcacccagtc aaaatgcgac tgtggccaca
     2461 gcagaatgcc acccgatgcg gcaatggaac gttgaccagg tttgccttcg caaaaggtat
     2521 taaagatagc gagttgtttc tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat
     2581 ggctgacgat attcaccggc tgacgaacgt ttttgcactg ggtcaggcga tgataaaacg
     2641 cctcagcctg ttccggcctt acgcccagac ggtgcatacc ggtatcgagt ttcatccaga
     2701 cggtaaccgg ctcgtccagg ctagcctctt ccagcgcagc cagctgttct tcgttatgca
     2761 cggcggtatg aaaatgttgc gcagaaatcg tcggcagatc tctggcatca aaaaagcctt
     2821 cgagtaacag tacaggtttg atgattcccc ccgcacgcag tcgcagagct tcttcgagac
     2881 gggctacgcc aaaggcgtca gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac
     2941 cataagcgtt cgctttcacc accgcaacca ttttactggc aggggccagt tcacgaagac
     3001 gttgcaggtt gtgtcgcaga gcgcggcggt taatcacaac agttgccgct tgcatttgtg
     3061 ttccttgata agtgtttgct ttaattacct aattcataaa ataattatta ttcgtcgtcg
     3121 tactgcggcc ccgcatagtt gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc
     3181 gtcccgattg ggccgttacg ttgtttaccg ataataattt ccgcgatgcc ttttaaatca
     3241 ctgttttcgt gatacacctc atcacgatag ataaacatga tcaagtccgc atcctgctcg
     3301 atagagccag attcacgcag gtcggagttg accgggcgtt tgtcggcacg ttgttccaga
     3361 gaacggttca actgggacag cgccaccacc ggcacgttca gttctttcgc cagtgctttc
     3421 agcgagcgag agatttctgc aatttccagc gtacggttat cggaaagcgc cggtacgcgc
     3481 atcagttgca ggtagtcgat catgataagc ccgatgccgc cgtgttcacg ggcaatacgg
     3541 cgtgcgcggg aacgcacttc cgttggcgtc aggccggagg agtcatcgat ataaatattg
     3601 cgtttttcga gcaaaatacc catggtgccg gaaatgcgcg cccagtcttc atcatcgagc
     3661 tgcccggtac ggattttagt ctggtcaacg cgcgacagcg acgccagaga acgcatcatg
     3721 atctgttctg atggcatctc cagcgagaag ataagtactg gtttatcctg caacatcgcc
     3781 gcgttttcga cgaggttcat cgcaaatgtt gttttaccca tcgacggacg cgcggcgacg
     3841 atgatcaaat ccgacggctg caagccagcg gtttttttgt tgagatcgtc ataaccggtg
     3901 tttactccgg taacgccatc gtgtggctgc tgaaacaact gctcaatacg cgccacggtt
     3961 gcgtcgagca catcggcgat gttcttcggc ccttcgtctt tgtttgcacg actttcggca
     4021 attttaaaga cgcgggattc agcaaggtcc agcagatctt cgctggtacg cccctgcgga
     4081 tcaaaaccgg cttcagcaat ctcattcgca accgagatca tctcacggac aacggcacgt
     4141 tcacgcacga tgtcagcata agcactgatg ttcgccgcac ttggcgtatt ttttgacagc
     4201 tctgccagat aagcaaaacc accgacgcta tcgagttgcc cctggcgttc cagcgattcc
     4261 gcaagggtaa tcagatcgat aggactaccg ctttcctgca aacgcgccat ttcagtaaag
     4321 atatggcggt gtgggcgggt gtaaaaatcg tctgccacca cacgctcggc tacatcgtcc
     4381 cagcgttcgt tatccagcat taaaccgccc aacaccgact gctccgcttc gatcgagtgc
     4441 ggaggcactt tcagcccggc aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg
     4501 ttgaagggtt tatttcctgc catagtgaat ggagttaccg agataaagaa tgggtcgaaa
     4561 ctttaccata tgaagcagac cctgacgata cgttctggag gacacatggc aacacgaatt
     4621 gaatttcaca agcacggtgg cccggaagta cttcaagccg tagagttcac tcctgccgat
     4681 ccggcagaga atgaaatcca ggtcgaaaat aaagccatcg gcatcaattt tatcgacacg
     4741 tatatccgca gcggccttta cccgccgcca tcactaccca gcggattagg taccgaagcg
     4801 gcaggcatcg tgagtaaagt cggcagtggt gtaaagcata ttaaggcagg cgatcgtgta
     4861 gtctatgcgc agtcggcgtt aggcgcttac agctctgtgc ataacattaa tgcggataaa
     4921 gcggcgattc tgcctgcggc aatttctttt gagcaagctg cagcatcttt cctgaaaggc
     4981 ttaacggttt attatctgct gcgcaaaacc tatgaaatta aacccgatga gcagttcctg
     5041 ttccacgcag cagctggcgg cgttggcttg attgcctgcc agtgggcaaa agccctgggc
     5101 gcgaaactta tcggcaccgt aggaaccgcg caaaaagcgc agagcgcgct aaaagcgggc
     5161 gcgtggcagg ttattaacta tcgtgaagag gatctggtcg agcggttaaa agagatcacc
     5221 ggcggtaaga aagtgcgcgt ggtgtacgat tccgtgggca gagacacctg ggaacggtcg
     5281 ctggattgcc tgcaacgccg cggcttaatg gtcagttttg gcaactcatc aggtgcggtt
     5341 accggtgtga acttaggcat tctcaatcaa aaaggctcgt tgtatgtgac acgcccttcc
     5401 ctgcaaggct atatcaccac gcgggaggaa ttaaccgagg ccagtaatga actgttctct
     5461 ttaattgcca gcggtgtgat taaggtcgat gtcgccgagc agcagaaata tccgctgaag
     5521 gatgcgcagc gtgcgcatga gattctggaa agccgggcga cgcaaggttc cagcctgttg
     5581 attccataaa agaaataggg cttccacctg ggaagccctt tctttttata gttcggctgt
     5641 atgtagggta cagcacgatg aatctgttag aggcgcaata gtgacagatt tgattatcaa
     5701 ttcctatttt gttctaagga taaaacctta ggttgtgatc atccgcacaa tcccttagta
     5761 acgccagcgg tcataacgct gatatttcgg cacttttggt gctttaatcg ccttaataac
     5821 ccacaccacc gcaatcgcca gtagtaacca cggcagcaac ttaatcatca atgccagcat
     5881 accgccgagg aacataatgg ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga
     5941 aacgccggtg accatcagca tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat
     6001 gctctcccaa atatgaaatc tcttgctggc attacaagaa tcatgccaaa aataatctat
     6061 tgatttaaca gcaaaacgcc ccgcgacggt gcgcagggcg tggtgaattt gactactttt
     6121 tggtgaaaag ttaacgctta tccgccacca gtttgagcgc gtgttccagc acattaatgt
     6181 ctgcacccgc tttatgggca ttttcactta aataacgccg ccactgccgc gcgccaggaa
     6241 taccctggaa caagcccaac atatgccggg taatatggcc gagatacgtc ccctggctga
     6301 gttcacgctc aatgtacgga tacatggcgc gcactaccgc caccggatcg gcatcggtat
     6361 ccgaggaacc aaagatctcc cggtctactg ccgccagaat acccggattc tgatacgcct
     6421 cgcgcccgac catcacgcca tccatatgtt gcaggtgtgc tttggcctct tccagcgact
     6481 tgataccacc gttaatcgac attgtcagat gcggaaagtc acgcttcagt tgatacacac
     6541 gcggataatc gagcggcggg atttcacggt tttctttcgg gcttaacccc gaaagccagg
     6601 ctttacgtgc gtggatgata aacatctcac actcgccttt gccggaaaca gtgttgatga
     6661 aatcgcagag aaattcatag ctgtcctggt catcgatgcc aatacgcgtt ttcaccgtca
     6721 ctggaatcga caccacatcg cgcatcgctt tcacacagtc ggcaaccagc tgcgcattac
     6781 ccatcagaca cgcaccaaac atgccgttct gcacccggtc agacgggcag ccgacattca
     6841 ggttgatctc atcatatccg cgcgcttctg ccagctttgc acactgcgcc agcgccgccg
     6901 gatcgctacc gcccagttgc aacgctaccg gatgttcttc ttcactgtac gccagataat
     6961 cacctttacc gtgaataatc gcccctgtgg tcaccatttc ggtatacagc aacgtattgc
     7021 gggaaagcag acgcaagaaa tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa
     7081 cgctaaaccg accactccag tgaacgtcag ttttttcagg cattgcgctg gtttggttga
     7141 ttttttgcat ttcagaatta ccgtgcattt tcaaatgtag agattatttt atcgatatat
     7201 catggggtta tgttattcag catcactatt caggaggctc aatagcgggg tactatacca
     7261 taacaacagg aagcgcctgt ctcattgcaa aagaaaattg agatcctctc aaggcatgaa
     7321 gctctcacga agtgatggaa ataatcttat tagccgttaa ctttgttaag gccaatgata
     7381 aacaatccag gttcgacgat aaataaaaaa tcacacatta aactctggtg atatatctcc
     7441 ctgctaatag cattgataga gaaaaaagaa cccaataaat attgggtcct tttatataat
     7501 gccttccata ctatcgaaga acttcacata ttatttctcc gatttaaccc cgaacaaatc
     7561 ataaattaat ttagaagtgt ctgtaagtat tttaatctct tcctttgagg ttgggtcaaa
     7621 cgacttcgcg aagctaatta attgtggtgc agcatctctc attttgctta aaatatcagg
     7681 atcgagcgtt ccttcattca ccagatgtga catcttatcc agatagccat caaaattcat
     7741 ttctccccca tcagccagac gcttcatctc tcccaggtac ttcttcatat cacgttgaga
     7801 taatttttca aactgagctt tcaggtagct ctcattattt tcattaatat acactgtttc
     7861 tgataaatct gcgtaggcac tagaatatga aagtgaaatt aacagcgaag caaataattt
     7921 taagctgttg tcatcatgtt cctggcaggc attaacaaac gttaaaaaac cagaacctat
     7981 tgcttgaaaa tgtatattcg ctaaaggttc attatcctta gattcctcat aaaatagctg
     8041 aaccgtggag ggaaggccat tcttagtttc tacatcaaaa gtacattgtt caatgggagt
     8101 cgcatcttta ttatccccag gattacatat actattaatc gcgaaatgaa aaatagcccg
     8161 gtcaacaact gatgcaataa agtcacgatc gtttaaatcc tcatcagtgc aatcgtcaat
     8221 atacttattg acccacattt tgagatcatt tatttgctca attaaaaaca cattttttta
     8281 cgtgcatgcc ataaattatg cgtgattcgt tgaataaaca aatctatttt tgcaataatt
     8341 atatcccgat gacgcagcgc tgcataatca atagatttac catatatatt
//
LOCUS       DAZFYC010000173         8384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFYC010000173 DAZFYC010000000
VERSION     DAZFYC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.174"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..1995)
                     /gene="yjcS"
                     /locus_tag="ACHKW5_004707"
     CDS             complement(10..1995)
                     /gene="yjcS"
                     /locus_tag="ACHKW5_004707"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO7513927.1"
                     /translation="MNNSRLFRLSKIIIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(2268..3197)
                     /gene="alsK"
                     /locus_tag="ACHKW5_004708"
     CDS             complement(2268..3197)
                     /gene="alsK"
                     /locus_tag="ACHKW5_004708"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO7513928.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(3181..3876)
                     /gene="alsE"
                     /locus_tag="ACHKW5_004709"
     CDS             complement(3181..3876)
                     /gene="alsE"
                     /locus_tag="ACHKW5_004709"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO7513929.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(3887..4867)
                     /gene="alsC"
                     /locus_tag="ACHKW5_004710"
     CDS             complement(3887..4867)
                     /gene="alsC"
                     /locus_tag="ACHKW5_004710"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO7513930.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(4846..6377)
                     /gene="alsA"
                     /locus_tag="ACHKW5_004711"
                     /pseudo
     CDS             complement(4846..6377)
                     /gene="alsA"
                     /locus_tag="ACHKW5_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
     gene            complement(6504..7439)
                     /gene="alsB"
                     /locus_tag="ACHKW5_004712"
     CDS             complement(6504..7439)
                     /gene="alsB"
                     /locus_tag="ACHKW5_004712"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO7513931.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVNAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(7498..>8384)
                     /gene="alsR"
                     /locus_tag="ACHKW5_004713"
     CDS             complement(7498..>8384)
                     /gene="alsR"
                     /locus_tag="ACHKW5_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO7513932.1"
                     /translation="QSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNLSC
                     APAIKDVAEALSVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDEAP
                     QDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQHK
                     FLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKIIC
                     ITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQANI
                     NMQKTGAIVDFFSPGALK"
BASE COUNT         2037 a         2121 c         2042 g         2184 t
ORIGIN      
        1 cgagtgacat taatttgggg ttacgatatt cacccacaaa tcgaaattat ccagacaggc
       61 gataatttct tccagtttcg cgccattgcc aataattttg gctttcttcg cttttaccag
      121 atccgccatt ttggcttgcc cggtcagcac ggcgtgcaga tcttcacggc tgatgtagaa
      181 agaggcgtca gcttgggatt gcagtgtttt acggtagtta agcacgctat cgttcagcgt
      241 caggttgagg ttatcgccat tgctcatatt gaagttcagg ctgatatttt tacccgcggc
      301 tttcgcgcta tcgaggcgaa cggacataaa gtcgaacagc atttcgaccg acatcccgcg
      361 aatggtgtcc ggggaaccgg tggtgccgtg gctgaactta tgcacccctt cgcgcagctc
      421 tttcgcgccg gtcaggtaga aaccgcgcca ggtggcggac tcggcctgat agcccagctg
      481 ttcaaagtta ttcgcttgca gattcttcgc gacctggtca cccgggttgg cggcaatcac
      541 ctgtttcagc agttctgccg accagcggta atcaccttgc ttgttcgctt cttgcgccag
      601 gttgatgaca cgggcagaac cgcccagcgc ctgcacgtaa cgtttaccca tctccacctg
      661 accatacgga tgcaggttag ccgggttacc gtcgtaatag ccaagataga agttatacac
      721 cgcgcgggcg ttgtggctga cagaaccgta atagccacgg ctggcccagt tattggcaag
      781 tgcaggcggc agtttaatca tgtcgccgat ttcattcatg gtgtagccct ggttcgccag
      841 gtgcagggtc tggtcgtgaa tgtacttgat agtatcgcga tatttaccaa tataatcatt
      901 gatatgctta ttgccccaga ccggccaggt gtgcggcata aacaggactt ccgcgtcgtt
      961 accccacata tccagcgttt cgttcagata ctcggtccac ttgctggtgt cgcgggtttt
     1021 cgcgccgcgt agagtgtaga agttgtgcag ggtatgcgtg gcgttctcgg cggtacacag
     1081 cgctttcagg gccggaatat agaagtgcat ttcggctggt gcttcgctac ctggggtcat
     1141 caggaagtca aactccaggc cgtcgataat catcttctca ccggttttgg tgatggtttt
     1201 gttcggtgcg atgatgctcg ggtagcccga tgccagcgtt acgccaagac cattgccgat
     1261 gttaccctga gggttatgcg ccagcagcag gccgtaggag taaagtgcac ggcggctcat
     1321 aatattaccc gccagtacgt tttcgctgat ggcttcgtcc ataaagccag caggggcgat
     1381 aacctgcact ttaccggatt tcacatcggc ttcagagata atgcctttca cgccgccata
     1441 gtggtcggta tggctgtggg tataaataac cgcaacaatc ggtttttgcg ggcgattttg
     1501 gaagtaaagg tcaagggcgg cttttgcgct cggtggcgtc accagcgggt cgatgacaat
     1561 aatcccggta tcaccttcga taaaggtgat gttggagata tcctgaccgc gaacctggta
     1621 cattctgtcg gtgactttaa acaggccgga aataccgttt aactgcgact gacgccacag
     1681 gctagggtta acggtttcgg gcgcgggcgc attgatatca aatttataat cttccccgcg
     1741 ataatatggt ttgccattag catcgcgcag aataccttca tccagcagcg gagcgataaa
     1801 tccacgctga gcgtcgtcaa aatcctgacg atcgctaaac ggtaatgatt tggcgtaatt
     1861 ctgattaacc tgttgggtat attgagtggc ggctttcgct tcatctgttg cgtaagcggt
     1921 atttaccatc atgccgctgg cggcagttaa cgcaataata attttgctca aacggaataa
     1981 ccgagagtta ttcatataaa tctccattta tcatttatgt cgtaaatatg taatccatgc
     2041 aaaatacgtc atttaaagct ggggttatca taattgggaa tttgcgactt taataagtgg
     2101 aagtgtgagc tgaacgatcc attttattag gcatttgtgc tgaagaggaa aagttacagt
     2161 aaaactatca ttgcagaaaa taatatgagc cgttttatgt gtttattata tatattgtac
     2221 ctgtcgttgg cacagcggga cattgcgcta ctgtgccaac gctttactca tggggcttta
     2281 gcacaggact gtggcaaaaa atgttgatgc gccaatattg ctgcaccctg agcgccatta
     2341 aagtcagaag atgaggcggc aataaagcgc acgacctgat gcggcagtgg acggcgcagg
     2401 tacttttggg tcatggcaat gagagtctcg cgtgggaagg cgggcatatc catcacgcca
     2461 ccgcccagaa tcaccgcatc gggatcgaac agattaatgc tggtggcaat ggcccgtgcc
     2521 gcgttttcaa gcagactctg gacgaaaggg gcgttttccg catggacgaa aaggtcgctc
     2581 aatgggtaat ttcggggctg ttgttcgtac cagcgtctta gcgccattcc agagcaattg
     2641 gtttccaggc acccaggatt gccacacgcg cagtgttggg tcatatctcc cagggggata
     2701 tgacccagtt cgcctgccac accgtgtgca cccgtccacg gcgcaccgtt catccatact
     2761 gcgaacccca tccccgtacc gagataggcc gccagaacca gttgttgcgt aaggcggttt
     2821 tctactacgt cccaggagag ttgcaggtta acgtcgcggg aaaactcaac cggacaattc
     2881 agcgtatttt cgagcttatc ggcgagatca tataaatccg ccgctgttaa cggcaggtta
     2941 ggcgtagaaa ttatggtgcg tttatcttta ctgaccagcg ccggaaatcc catcaccaga
     3001 ccatgacagc gagcgttaaa gcgcctgagt tgctcgtcaa tcatttcgcc gatacccgac
     3061 accaggccgg gagcaatgac ttctgcggtc cgcttttttt cgcagtgtag cgtttcacct
     3121 tctgctgtcc gcagacaaaa gcggatatgc gttgccccca tatccacgcc cgctacgacg
     3181 ttatgctgtt tttgcatgag gctgtacctc gctttttgca gccagaatct gcgtggtcat
     3241 aattctccat gcttcgtcga tattttccgc atgattaaac aggccggaag tgccgacgat
     3301 aaagacatcc gcccctgccg ccatcagttt ttcgtaagtt gcctggttgc aggaaccgtc
     3361 cacctcaatt tcgtactcca gaccttctcg ttcacgccat gccttcagtt cggcaagttt
     3421 atccagcatt tcaggaatga acggttgtcc ggcaaagccg ggatcgacag tcatgaccgt
     3481 aattttatcg gccttatgga tatagtattt catggcctca actggtgtct ccgggttaag
     3541 gatcagcccc actttcatgt catgacagcg gatttcatca atcaggcgga acgcctgacc
     3601 gttgatagtt tccggatgca aagtgatgaa atcagctccc gcacgcgcca gttgagcaat
     3661 gtaatcctgc ggccgcgtca ccatcagatg acagtcgagc ggtttagttg ccagtttttt
     3721 aacctgactt acgaagaacg gtgagagtgt cagattgggg acaaagtgac catccatgat
     3781 atcgatgtga aagtaatcgg catggctgtc gataaattcg atctgttctt taaatttcag
     3841 cagatccata cacattaacg agggggagat tttcatgttc aattccttac ttactgataa
     3901 gacggtcaag ggcgacagcc gcgataatta atccgcccat caccaccagt tggtaatagg
     3961 tttgtacctg caaaatattc agaccgttgt tgatggtgcc gatgatcaac ccgccaatca
     4021 ccacagagaa aatgcgcccc ttgccgccga agaaactggt gccgccaatg atggcgctgg
     4081 caatggcata ggtttcaaaa cccataccgg caagcggttc tgcggcaccg agtcgtgcag
     4141 ttgagacgac gcctgccaga cctgcacaaa caccagagat gataaacacc accaggatgt
     4201 ggaatttcac gtcaatcccg gaatagaacg ccgaattttt gttgccgccc agtgcgtaga
     4261 tgttgcgccc gagccgcata cgcgttgtca gaaaccaaag gatgagcgcg acaattagcg
     4321 agaagataac ggggacaggt atcccaatta cgctggcggc aaagaagttc acgaagtcaa
     4381 aggagaagcc gtataccgag ttggcatcgg agatcaccag cgtgatccca cggaaaatcg
     4441 cgttggtgcc aagggtgatg atgaacgggt gtagccccgt ccagttgacc aggcagccgt
     4501 tgatcgcccc cagtgcgccg ccaaccagta caccgccaat catcgctgcg agaaacgggt
     4561 caacacctgc caacatcagt ttggcggtca ccataccgga aagcgccaga atcgcgccaa
     4621 ccgagaggtc gataccagcg accaggatag cgaaaaactc gcccatgccg atcaataccg
     4681 tcacggagct ttgaacaaaa atctgggtaa tattattggt ggtcagaaaa tattctggcg
     4741 acagcgaacc aaaaatggcg acgatgatcg ccaggataaa aaaggtgccg tatttatccc
     4801 agaacagcgc aaagttgaac ggtttcttct cgctcgcttc gctttttact cttgtggtaa
     4861 agcccatgcc ataatctcct cttcgctcat gtcatcgcga ttcgtcagga tttgcgtcag
     4921 tcgtccttcg cagaacacgg cgatgcggtc gcagacggtg ataatttcag gtagttcaga
     4981 tgacaccatc aggatgactt ttccgtcgtc cgccagttgg cgcatcactt tgtaaatttc
     5041 ggctttcgcg ccaacgtcga tgccgcgggt aggttcatcg aaaataatca cttccgggca
     5101 acagcacagc catttggaga tcaggacttt ctgctgattt cccccggaga gttcggtgat
     5161 attctggttt accgaatgac atttcagcgc cagcagttcg cgttgatttt cagcggtacg
     5221 ttgctcgtca acttcatgaa acaagcccat cgcgccttta tagccgccgt ctttcagact
     5281 gcggctgatc gccatgttct gagcgatgga aaagttggga aaaaaaccgt tatcccggcg
     5341 gctttcagtg atgtaagcca tccctttttt cactgcatcc aggggggaac gtggagagat
     5401 atctttgcca ttaagacgga tttctccgcc agcgcgttta tcaacgccaa acagacaatt
     5461 catcagttca gtacgtccgg aaccgaccag cccggcaaag cctaatattc tccccggcag
     5521 acgctaaatg agatatcccg gacctttttt ctgtcacggc tggtgacgtt gcgcacctca
     5581 aaaaccgtat cgtgcacaag gttgctgaca ttctccttca tcgcgttaaa acggttttgc
     5641 agttcgcggc ctaccatcag acggacgata tcgtcatttg acacatcgct taccatgccg
     5701 ctgcaaacgc tgctgccgtc tttcatcacc gtatagcggt cgcaaatacg gcgaatttcc
     5761 gccaacttat gcgagatata gacgatggcc gtaccctctt tacgcaactg attcatgatc
     5821 agaaacagat agtccacctc tttattggtg agtgaggagg tgggttcatc catgatgatg
     5881 actttggcat cgagcatcag cgttttggca atttccagca tctgcttgtg gctgatagat
     5941 agattcgcca ctttctcatc taaatcaact ttcaagccca cgcgtaataa catcatggcg
     6001 gcgcggacac gcatttctcg ccagtcgata atattgacgc cacagatttt tttcgtcaga
     6061 tgacgaccaa tatataaatt ttccagtacg gttaattcat caataacgct gagttcctga
     6121 taaataatcc cgataccgag ttgtgccgct aatttatgat ccagcttgtt atagctaatg
     6181 ttattaatgg taatggtgcc tttggtcggc tcatgtattc cggataaaac cttcattagc
     6241 gtggatttac ccgcgccatt ttctcctagt aatgcatgta tttcaccagg ataaaccgtt
     6301 aaattaaccg actttaatgc gtgaaccgga ccaaaggact tgccgatccc cgccatcgat
     6361 atatatggcg tggccataaa taacctctct gcacgaaata tcgtattatt gcctgatggc
     6421 tacatttatc aggtctacgt tcaggttatt ttgttggccg gataagacac ttcattagca
     6481 tcatcatccg gcatcattca gttttattga gtgaccagga ttgaatcgac cagtttaaat
     6541 tccggtgctt tatccagcgg gataaccttg ccggatttct cagcgttaac catcagcttc
     6601 agacccgttg cgccaatatc tgccgggttc tgcgcaaccg tcgcggtcat ttgtccggct
     6661 tccaccattt tgcgggcttc cggaattcca tctgtaccga cgaccaatat tttgcccgtt
     6721 tttccggcgt ttgcgactgc ctgagcaaca cccattgcca tcgtgtcatt cgcgcaatag
     6781 atcgctttaa tattcggatt acgttgcaac acgttagtgg cgacatccag tgctttaatg
     6841 cggtcccagt cagcgggctg gctggcgaca agcttgatct ggcttgcttt tttgaaggct
     6901 tcggtggcac cattacgacg cgcttcaccg gaggcgttac cggctttacc ctcaatgatt
     6961 gcgacttcac caccttcagc gcccaatttg tcaataatga acgacgcgcc tttcgccccg
     7021 acagcaacgt tatcggtggt gacaaaagct tccacattgc cgccagcttt tttcagatta
     7081 tccatgtcga ttttttcatc gagattaacc agataaatgc cttttttcca tgcgcgggcg
     7141 acaggcatga ccagattcac tgaggataat ggagcgaagg cgataccttt gtaattttta
     7201 ttactgagat cttcaaataa ctgcaattga gattgaaaat cgccttctga aggagaggca
     7261 aaaatatcaa cgctgacgcc cagtgttttt gcttcatctt caatgccttt tttcatatct
     7321 acccaaaatg ggttggagag ggttttcaat acgacagcat attcggcggc agcaaaagcg
     7381 ctggttgaca acattaagcc cacgagtgtg ccgctgaaat atttcagata tttattcata
     7441 ataatgttct cacggtaagg ggacaggtaa ctccgcgatg gcggaattac ctttttctta
     7501 tttcagcgcg cctggtgaga agaaatcaac aattgcgccg gttttttgca tattaatatt
     7561 agcttgttca atgttgagct gggcgacaga gacaaaaaaa gcgtccagca aagttagttg
     7621 taatattctt gccgaggcat tacgacctaa taacggcgtt tccggggctg gtgagcaaat
     7681 aatataatcg gccagtttcg ctatcggtga atggtagcta tgggttatac aaataatctt
     7741 tgccccgttc ttttttgcca gttctacggc cgcttttaca tcactggttc gcccggaatg
     7801 ggtcactacc agcacaacat ctccttcctg taacaacgaa gcggacatca tcatgatgtg
     7861 agcatcagga taggcctgac agcgtacgcc aatgcgcaag aacttgtgct gtacatcagc
     7921 acagatagca tttgatcctc cggcaccgta caaatcccgc tgtctggcct gatagaaaaa
     7981 gcgggcggca cggtggatct catcaacgtt gacgatcgac tgaccttcca taatggtgcg
     8041 taaagtgatg ttaaatacct tattcaccac atcctgcggc gcttcatcaa aagccaactc
     8101 ggaaggcaat acctgttctg actgagaaaa ataatcttcc agcgcactgc gtaagttacg
     8161 aaagccgcta aaccccagca gctttgatac cttaactatc atcgcttcag ataccgacag
     8221 agcttctgcg acatctttaa ttgcgggtgc acaactcagg ttaccgggtt tgagtaacca
     8281 ctctacgatg cggctttcat tttctgtcat tccttcctgc ttcattcgca ggtaaggcgc
     8341 taaccctata ccgttcggaa gcgctgaatc aaactctgac tggc
//
LOCUS       DAZFYC010000174         8352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFYC010000174 DAZFYC010000000
VERSION     DAZFYC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.175"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            348..1376
                     /gene="torT"
                     /locus_tag="ACHKW5_004714"
     CDS             348..1376
                     /gene="torT"
                     /locus_tag="ACHKW5_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO7513933.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1349..2041)
                     /gene="torR"
                     /locus_tag="ACHKW5_004715"
     CDS             complement(1349..2041)
                     /gene="torR"
                     /locus_tag="ACHKW5_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO7513934.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2161..3333
                     /gene="torC"
                     /locus_tag="ACHKW5_004716"
                     /pseudo
     CDS             2161..3333
                     /gene="torC"
                     /locus_tag="ACHKW5_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
     gene            3333..5879
                     /gene="torA"
                     /locus_tag="ACHKW5_004717"
     CDS             3333..5879
                     /gene="torA"
                     /locus_tag="ACHKW5_004717"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO7513935.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGVIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            5876..6475
                     /gene="torD"
                     /locus_tag="ACHKW5_004718"
     CDS             5876..6475
                     /gene="torD"
                     /locus_tag="ACHKW5_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO7513936.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(6568..6873)
                     /gene="cbpM"
                     /locus_tag="ACHKW5_004719"
     CDS             complement(6568..6873)
                     /gene="cbpM"
                     /locus_tag="ACHKW5_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO7513937.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(6873..7793)
                     /gene="cbpA"
                     /locus_tag="ACHKW5_004720"
     CDS             complement(6873..7793)
                     /gene="cbpA"
                     /locus_tag="ACHKW5_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO7513938.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            8054..>8352
                     /locus_tag="ACHKW5_004721"
     CDS             8054..>8352
                     /locus_tag="ACHKW5_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513939.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGV"
BASE COUNT         2065 a         2049 c         2247 g         1991 t
ORIGIN      
        1 ttgattctgt ttaggcatag ccataatgca tgatggtgtg gcgtggcaaa gcagagatat
       61 acaaagtctg ccacatgttt ttatgacaaa aaattagcgc aagaagacaa aaaatcacct
      121 tgcgctaatg ctctgttgta ggtcactaac cacatctaac tcattgatta atagtgactc
      181 tagatgtagt gttttatcgt attttgcagt ctgttattta atcatgaatt catgtaacaa
      241 tatgattttt aaaatatttg tgtgtatttg tcatccttta ggtgaaaaag gttgagtcgc
      301 aaagcggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
      361 tatttttact tctttccctt ttcatgttat cggcattttc ggctgataac ctgttgcgct
      421 ggcatgatgc gcagcattac acggtgcaag cctctacgcc gcttaaagcc aaacgcgcat
      481 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
      541 gtatgcagga ggcagctcgc cgctatggtg tggatttaaa agtgctggag gcaggcggct
      601 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
      661 ccattttgct cggtagtagc actacgtcat ttcccgacct gcaaaagcag gtagcaaatc
      721 tgccggtgat cgaactggta aatgctcttg atgatcccct ggtgaaaagc cgcgttggtg
      781 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
      841 aaccactgaa cgtgctgttg atgcccggac ccgataatgc cgggggcagt aaagagatgg
      901 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
      961 gtgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggaa cgccacccag
     1021 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     1081 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     1141 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     1201 cggttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     1261 cgattttagt tctgacgccg aaaaatgccg accgtgaaca tattcgccgc tcgctgtcac
     1321 cagggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     1381 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     1441 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgtagcagac
     1501 gttcacggct gagaatttcg cccggattcg tcacaaatgc caccaacatt tcatactctg
     1561 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     1621 aataaccggc aaagcgatag cagttgtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     1681 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     1741 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     1801 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     1861 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     1921 tttcccgcag cccggcaccg ctcgctgtaa cggaaacggt atacccctcc tgagtgaagt
     1981 aagattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg tgatgtggca
     2041 tcagagggtt ttactcattc tgttcatatt ctgccgtaag ccgttcatcc tgaccagtgc
     2101 cgctgttcat atttgctcat taagatcgct tcactaaacc ataattttac aggggctatt
     2161 atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt actggcgctg
     2221 gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt tgggatcaaa
     2281 gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa
     2341 tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt
     2401 catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag caatgatatc
     2461 taccagacct ttattgctca ctccattgat acacctgaaa aattcgaagc caaacgcgcg
     2521 gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca actcggcaac ctgccgctcc
     2581 tgccataact acgatgcgat ggatcatgcg aagcagcatc ctgaagcggc acgtcagatg
     2641 aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat tgcccaccag
     2701 ttaccggata tgagtagcgg cttccgtaag cagttcgatg agctgcgcgc cagtgctaat
     2761 gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc gaaaggcgat
     2821 aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa acgtgacggc
     2881 gactggctgc aaattgaaat taccggctgg acggaaagcg ccggacgtca gcgtgtactc
     2941 acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt gcagcagcag
     3001 gtaaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag caagttgcag
     3061 gccactgcgt gaatgaagaa aggcgacatg gtgaacgata tcaaaccgat ctgggcttat
     3121 gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga aatcgcccac
     3181 tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac cagtctcgat
     3241 aaacgtgaag aacgcacctt gttgaaatat ctgcaaatga atgcgtctga caccgcaggt
     3301 aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc tttcaggcat
     3361 cacgtcggcg ttttctggca caactcggcg gcttaaccgt cgccgggatg ctggggccgt
     3421 cattgttaac gccgcgccgt gcgactgcgg cgcaagcggc gactgacgct gtcatctcga
     3481 aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgacggtg aaggatggtc
     3541 gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg attgccggat
     3601 tgccggatca cgtacacaac gcggcgcgta ttcgttatcc gatggtacgc gtggactggc
     3661 tgcgtaagcg ccatctcagc gatacctccc agcgcggtga taaccgtttt gtgcgcgtga
     3721 gctgggatga agccctcgac atgttctatg aagaactgga acgcgtgcag aaaactcacg
     3781 ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac ggggatgttc cataacgctt
     3841 cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg ggcggagatt
     3901 actctaccgg tgctgcgcag gtgatcctgc cgcgcgtggt tggctcaatg gaagtgtatg
     3961 aacagcaaac ctcctggccg ctggtattgc agaacagcaa aaccattgtg ctgtggggct
     4021 ctgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat
     4081 attacgcgca gctaaaagcg aaagtcgccg ccggtgaaat tgaggtcatc agcatcgatc
     4141 cggttgtcac atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta
     4201 acccgcaaac tgatgtgccg ctgcaactgg cgctggcgta tacgctgtac agtgaaaacc
     4261 tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc ctgccgtacc
     4321 tgctgggtga gaaagacggt cagccgaaag atgccgcatg ggctgaaaaa ctgaccggca
     4381 ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcaaatta
     4441 ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg
     4501 ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggctttggt tttggctggc
     4561 actataacgg cgcaggcacg ccggggcgta aaggcgttat tctgagtggt ttctccggct
     4621 ctacgtcgat tccgcctgtt cacgacaaca gtgattacaa aggctacagc agcaccattc
     4681 cgattgcccg ttttatcgat gcgatcctcg aaccggggaa agtgatcaac tggaacggta
     4741 aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc cggaactaac ccattccatc
     4801 gccatcagca gatcaaccgc attattgaag gctggcgcaa gctggaaacg gttatcgcca
     4861 tagataacca gtggacctca acctgccgct ttgccgatat cgtgctgcct gcgaccacgc
     4921 agtttgagcg taacgatctc gaccagtacg gcaaccactc caaccgtggc gttatcgcca
     4981 tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga ctttgatatt ttccgcgagc
     5041 tgtgccgtcg ctttaatcgc gaagaagcct ttaccgaagg gctggacgaa atgggctggc
     5101 tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg acgcggcgtt catctgccag
     5161 cgtttgatga cttctggaat aacaaagagt acgtcgagtt tgaccatccg cagatgtttg
     5221 ttcgccacca ggcattccgc gaagatccgg atctcgaacc gctgggcacg ccgagtggcc
     5281 tgattgagat ctactcgaaa accatcgccg atatgaacta cgacgattgt caggggcatc
     5341 cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc cggctcgcag aagtatccgt
     5401 tgcatctgca atctgtgcat ccggatttcc gacttcactc gcagttatgt gagtcggaaa
     5461 ctctgcgtca gcaatatacg gtagcgggta aagagccagt gttcattaac ccgcaggatg
     5521 ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt ctttaacgct cgcggtcagg
     5581 tgttggcagg ggcagtagtt tctgaccgct atgcacccgg cgtggcacga attcacgaag
     5641 gggcatggta cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa tacggtaacc
     5701 ccaacgtgct gaccatcgac atcggtactt cgcagctggc gcaggcgacc agtgcgcaca
     5761 ctacgctggt ggaaattgag aagtacaatg gagcagtgga gcaggtgacg gcgtttaacg
     5821 gccctgtgga gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg aaatcatgac
     5881 cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg ctggcgcagt tgttctcccg
     5941 tgaactggac gatgaacaac tgacgcaaat cgccagtgcg cagatggctg aatggttttc
     6001 gttgctgaaa agcgaaccgc cgctggctgc ggcggtgaat gagctggaaa actgtattgc
     6061 cacgctgaca gtacgtgacg atgcccgtct ggaactggcc gctgactttt gcggcctgtt
     6121 tctgatgacc gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac aggacgagca
     6181 agagattaaa cgcttgttag ttgaggcagg gatggaaacc agcggcaatt tcaacgaacc
     6241 ggcagaccat ctggcgatct atctcgagtt gttgagtcat ctgcattttt cgctgggaga
     6301 ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa aaaacactga cggcgctgtg
     6361 gcaatggtta ccagagtttg ctgcgcgttg ccatcagtat gacagctttg gtttttacgc
     6421 ggcactaagc cagttattgc tggtgttagt ggagtgcgac caccaaaaca gataacgtcg
     6481 tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc attatggcgc agttgccgga
     6541 tgcgtcatca catccggcaa acacacatca cggatgagct acaaaccggg aaagccgctg
     6601 gcgtagcagg cggttttcct gcttcaggtg tgcaatatca tccattaacg tcagcgccac
     6661 cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgtaccgcgc gttgcaccac
     6721 aatggcggca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg gttcaaccac
     6781 ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca ggcaaaattc
     6841 ggtaatagta aaagtcaccg taacattagc catcatgctt tcccccaatc tttacgtgga
     6901 tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt ttcatccggt
     6961 ttcggcggca tcacgatttt cagtaccgca tacagatcgc cggtctgttt tttgctcacc
     7021 agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg gatagtcagc
     7081 aaaatgcttt ctttcagtgt tggaacggtg actttagcac ccagcgccgc ttcccacggg
     7141 ctaaccggca ccacaatttc cagatcctgg ccgacaatat cgaacagcgg atgtggcgca
     7201 atatgaatca ccagccacaa atcgccattt ggaccaccgt tttcgcccgg cgtcccctgg
     7261 cctttcagac ggatgcgttg accattgccg acgcccgccg ggatcttcac attcagcgtt
     7321 ttcgggattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt atagctgatg
     7381 gtacgcttat gctcagtcag cgtttcttcg aggaataccg ccacttcgat ttcaatatcg
     7441 tggccgcgtg cggcggggcg ttgacggctc tggcgggcat gctgaccgaa aattgacgag
     7501 aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt cgccatgatg gaactgacgg
     7561 ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg acgttgttca
     7621 tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc atccggttct
     7681 ttgctgacat caggatggta tttgcgggca agtcgacgat aggcggtctt gattgtcttg
     7741 agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc catagcgtta
     7801 tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta taagtgtagg
     7861 gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat cgcggagagg
     7921 aggtcgccat caagatgggt tactgaacat attttaaaca ggtgaaaaag ggtgagcgat
     7981 ttttgatagt tgaaccaggc actttaagtt taactggggc gtcattattt attaaatttt
     8041 atagacgcta tatatgggta gtaatataca tggaattagt tgcactgcaa ataattattt
     8101 gaaacaggcc tggaacgata taaaaaatga gtacgaaaaa aatcaaacat attcaatcac
     8161 gctttttgaa aacacactgg tgtgttttat gcggttatac aatgaactca gacgtaaagt
     8221 aaatgaagag gatactccat gtctggaatg tgaatcacta gaaaaagaat ttgaggaaat
     8281 gcagaatgat aatgatctat cattatttat gagaacattg cgtactaatg atacacaaat
     8341 ttattcaggg gt
//
LOCUS       DAZFYC010000175         8336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFYC010000175 DAZFYC010000000
VERSION     DAZFYC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.176"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(115..191)
                     /locus_tag="ACHKW5_004722"
     tRNA            complement(115..191)
                     /locus_tag="ACHKW5_004722"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Asp,seq:gtc)
     gene            complement(324..1055)
                     /gene="dnaQ"
                     /locus_tag="ACHKW5_004723"
     CDS             complement(324..1055)
                     /gene="dnaQ"
                     /locus_tag="ACHKW5_004723"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO7513940.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1120..1587
                     /gene="rnhA"
                     /locus_tag="ACHKW5_004724"
     CDS             1120..1587
                     /gene="rnhA"
                     /locus_tag="ACHKW5_004724"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO7513941.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1584..2306)
                     /locus_tag="ACHKW5_004725"
     CDS             complement(1584..2306)
                     /locus_tag="ACHKW5_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7513942.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2340..3095
                     /gene="gloB"
                     /locus_tag="ACHKW5_004726"
     CDS             2340..3095
                     /gene="gloB"
                     /locus_tag="ACHKW5_004726"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO7513943.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3167..4525
                     /gene="mltD"
                     /locus_tag="ACHKW5_004727"
     CDS             3167..4525
                     /gene="mltD"
                     /locus_tag="ACHKW5_004727"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO7513944.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4573..5343)
                     /locus_tag="ACHKW5_004728"
     CDS             complement(4573..5343)
                     /locus_tag="ACHKW5_004728"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7513945.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTLEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5421..6221)
                     /locus_tag="ACHKW5_004729"
     CDS             complement(5421..6221)
                     /locus_tag="ACHKW5_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7513946.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6462..7376
                     /gene="yafC"
                     /locus_tag="ACHKW5_004730"
     CDS             6462..7376
                     /gene="yafC"
                     /locus_tag="ACHKW5_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO7513947.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7373..8176)
                     /gene="dkgB"
                     /locus_tag="ACHKW5_004731"
     CDS             complement(7373..8176)
                     /gene="dkgB"
                     /locus_tag="ACHKW5_004731"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO7513948.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2230 a         2002 c         2019 g         2085 t
ORIGIN      
        1 acatttacag cataacaaaa ccatagaccg aagaaatagc gggatttaaa tgcaaaaaaa
       61 aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt ttcatgagtg aatttggcgg
      121 aacggacggg actcgaaccc gcgcccccct gcgtgacagg caggtattct aaccgactga
      181 actaccgctc cgcgttgtgt tccgttggga acgaggcgaa tattacggat tgcctcaccc
      241 ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc aaaaatcgcc caagccgcta
      301 ttttcagcgc cttttacagg tatttatgcc cgccagaggc agcttccgcc cttcttctcc
      361 accagatcaa gacgggcttc atgagctgca agctcttcat ctgtcgcaaa aacaacgcgt
      421 aacttacttg cctgacgtac aatgcgctga attgttgctt caccttgttg ctgctgtgtc
      481 tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag ataaacttcc
      541 gcaaggatct gtgcatcgag taatgccccg tgtagcgttc gtttactgtt atctatttcg
      601 tagcgggcac ataacgcatc gaggctgttg cgcttaccag gaaacatttt cctcgccacc
      661 gcaaggctat cggtgacctt acagaaggta ttggtcttcg gaatatcgcg cttaagcaac
      721 gaaaactcgt agtccataaa gccgatatcg aacgctgcgt tatggatcac caactccgcg
      781 ccgcggatat agtccatgaa ctcatcggct acttcggcaa acgtgggctt atcgagcaaa
      841 aattcatcgg caataccatg tacgccaaag gcttccggat ccaccagccg atcgggtttg
      901 agataaacat ggaagttatt gcccgtcagg cgacggttca ccacttcaac ggcaccaatc
      961 tcaatgatct tgtggccttc atagtgcgca ccaatctggt tcataccggt ggtttcggta
     1021 tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt cagacttatc
     1081 gttttacagt tcgattcaat tacaggaagt ctaccagaga tgcttaaaca ggtagaaatt
     1141 ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg gttacggcgc tattttacgc
     1201 tatcgcggac gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa caaccgtatg
     1261 gagttgatgg ccgctattgt cgcgctggag gcgttaaaag aacattgcga agtcattttg
     1321 agtaccgaca gccagtatgt ccgccagggg atcactcagt ggatccatag ctggaaaaaa
     1381 cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg tcgatctctg gcaacgtctt
     1441 gatgctgcat tggggcagca tcaaatcaaa tgggaatggg ttaaaggcca tgccggacac
     1501 ccggaaaacg aacgctgtga tgaactggcc cgtgctgcgg caatgaatcc cacactggaa
     1561 gatacaggct atcaagtcga agtttaagcc tgtggtttac gatattgccg ggtggctcca
     1621 accgcctggc gaattcgtgg cttgttttta ctctgtttca tcggatttag cgttaaagga
     1681 atagtccgtt tccgggcaac aataagttgt aagcaaccaa gcgcaggaat atgtgcattc
     1741 aatagttttc caccgtgttt gttccacggg agaacgtgga aacggctggc gtgcagcact
     1801 tcaaaattca acaaagagag ccagtccagt tgccgcatca gagtaaacat ccggctgtta
     1861 tagggcgagg ttttgcgcaa taccggcaca agtttgcgta atcccattaa actgatggga
     1921 ttgaagccac taatcaccag ccagccatca tcaatcaata cccgatcggc ttcacgcaat
     1981 aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca gtagacaaac atcaacggat
     2041 ttatcggcaa aaggaagatg aagtgggtcc gcctgtacct ggacgggcat tccttgcgca
     2101 gaaacattca cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc gcttaaattg
     2161 ccaatcttaa gcagatgaaa accatacatt ttagtgaacc acgggttgag ctggcgctcc
     2221 agcgccttgc gataaagctc tccccagggc aaatcgcccc agcaatcagg agccacgaca
     2281 gtttgaggga ctcttgccgg tttcatcaca accttccgtt tcacactaag aggtaatcta
     2341 tgaatcttaa cagtattccc gcctttgatg acaattacat ctgggttttg aatgatgaag
     2401 caggtcgctg cctgattgtc gatcccggag acgcagagcc agtattaaac gccattaccg
     2461 ccaataactg gcaaccggag gccatatttc tcacccacca tcatcacgat cacgttggcg
     2521 gcgtaaaaga actggtggaa aagtttccgc aaattgtggt gtatggtcca caagagacac
     2581 aagataaggg aacaacacag gtagtcaaag atggcgaaac tgccttcgtt ttggggcatg
     2641 aatttagtgt aattgccaca ccgggtcaca ctttaggaca tatctgttac ttcagtaaac
     2701 cttatctatt ttgcggcgac acgctgtttt ctggtgggtg tggtaggttg tttgaaggga
     2761 cagcatcaca aatgtatcaa tcacttaata agttaagtgc gttacctgac gatacattgg
     2821 tatgttgtgc tcatgaatat acgttatcaa atatgaagtt tgctttgagt attcttccgc
     2881 acgatttgtc cataaatgat tattatcgta aagttaagga gttacgggca aaaaatcaaa
     2941 taacactacc cgtaattctg aaaaatgagc ggcaaattaa tgttttttta agaacagaag
     3001 atattgattt aattaatgta attaatgaag aaacattatt gcaacaacct gaagaacgtt
     3061 ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc atcggctccg
     3121 ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga aggcaaaagc
     3181 gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggcaacg ttcaacagca
     3241 cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa gtcaggcacg
     3301 atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt tcattggcga
     3361 cgagctaaag atgggaattc cggaaaatga ccggattcgc gaacagaaac agaaatattt
     3421 acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata tgtactggat
     3481 agccgggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac ccatagtgga
     3541 gagcgctttt gatcctcacg caacgtctgg cgccaatgcc gcaggcatct ggcagatcat
     3601 tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgacg cgcgtcgcga
     3661 tgttgttgct tcaacaactg ccgcgctgaa catgatgcag cgtctgaaca agatgtttga
     3721 cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg tcatgaaggc
     3781 aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac cgttgccgca
     3841 ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca aaaacagcaa
     3901 gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcgc gtgtgcacct
     3961 gagcagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg tcagcaagct
     4021 gaagacattc aacgctggcg tgaaaggctc cacgctgggc gcaagtggtc cgcaatacgt
     4081 gatggtgcca aagaagcatg cagatcaact gcgtgaatct ctcgcttcag gcgaaattgc
     4141 tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt aacagccgtg tttacaccgt
     4201 acgctctggc gacacgcttt caagtatcgc ttcacgtctc ggcgtaagca ccaaagattt
     4261 gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt tgacgattgg
     4321 tgcaggcagt agcgcacagc gactggcaaa caacagcgat agcattacgt atcgtgtgcg
     4381 caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca aagatgtgat
     4441 gcgctggaac agcgacactg cgaatctgca accaggcgac aagctgacgt tgtttgtgaa
     4501 aaacaacaac atgccagatt cctgacaaac cagataataa aaaggcaccg attcccccgg
     4561 tgcctttttt atttatgctg ctttatgtgc ttctaccatg atgatatcac tggtgaaaga
     4621 gccatcattc tgcaaggcaa aatacgttct cacctctgtc gatgcgctct gttggtaaat
     4681 gcgaatagcg tctactaacg cttctagcgt acgcattctc gcgacccatg aagaaaattc
     4741 cagcggtaac ttatctgtaa ttaaattatc aactatcaga ttggcttcat tgattaacct
     4801 caaccactca ccgctggcgt agtttcgtac gtgagaggta tcgcgtaatg cttctaccgt
     4861 ctgtaaccag atgtcgcgca ctggatgacc cggagacatt acgtccatca caatcatcct
     4921 accgccaggt ttcaatatcc tattcacttc tcgcagtgct gcaccaacat catgccagtg
     4981 atgggcagaa taacggctga taacaatatc aaatgcgtta tcggcaaatg gcagactttc
     5041 ggcataaccc tggcgggtgg tgatattttt cagttgccgg gcttcggcag cttgtgccac
     5101 gacatccagc atttgggcag ataagtcata cgccaccacc gcgctcacgt tctgcgcagc
     5161 gacaaagctg gcatgccctg ctccgcagcc catatcaagt acacttgcat caggataatc
     5221 agccagacac accgccaggc gctgtaaatc tcgcccggat gcatgtacgg tactggttaa
     5281 atactcactg gcctgcgagc taaactgctt ttctacgtgg tcatggtggg attgtgttgt
     5341 cattgcgctg tccttttgtt gtttgagtat aaaaggacag cgccaaacgt tggtctgccc
     5401 tgtggcagac ctgacatacc ttatttatca ggcttgccgg gactgaattc aacgagtagc
     5461 ggattgtgat cggaagcgcg cgtaaccaat acagaagctt cgctgacgtt cagaccacgg
     5521 tagaaaacaa aatcgagcgg gcggccaaac gcccgacggc gctgatcatc ggtaaaacgc
     5581 acctggcgca gcgacatttc ccgcgcaaag cgatataacg cgttcatcct tctacggctc
     5641 caggcattga aatctcctgc cataatgacc gggccgctgt ggtgagctat ctgatcacca
     5701 ataggaagta actgcttact atagacatcc acgcccagac tgaagttgac ggcgtgtata
     5761 ttaaccacca tcaacaggcg ggtgtcaggt aatggataga ccgtcaccag tgccgacttc
     5821 gccagacgca aaatgggttc tcgttcacgt aacgggcagc aatacactgg atgcgccgcc
     5881 gaaagggtca ttacgccaga aggatgttgt ggcagtacga aagcgggtac ctgatcggcg
     5941 gcaagatagt tagcggtcgc aaactgtact aactctggcg ttgtctgcgc ttcctgcaat
     6001 aacaccagat gtgcatcttt gccgtagttc tttaataccg acaaccattc agcgcgttgc
     6061 tgtttgtata tgttccacac caggatccga atacgctctt cggtacttaa cggttctccg
     6121 ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt
     6181 tgtccggcaa catagcgcat ggcataggtg ttttttcgca cttttgactt tgaaacctct
     6241 attacgtcaa cccttccgca ctcgggaggg ttctactgtt atagggattt tagctcactc
     6301 tttcaacgag caattgtata ttgttatgta agcaagtgct tacaccggtc agaattttgc
     6361 tcattatgga gaacattaaa tcgcatgaca tatcacgcat cctgatggtt taatgacaaa
     6421 taaattcaaa atatctcttc tattttgcaa caggagcaaa aatgaaagcc acgtcggaag
     6481 aactcgccat ttttgtttcg gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat
     6541 tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa gctggagatg aaacttggcg
     6601 ttagcctgct taatcggacc acgcgacaac ttagcctgac ggaagaaggc gagcgttatt
     6661 ttcgtcgcgt acagtcaatt ttgcaggaga tggcatcggc agaatcagaa attatggaga
     6721 cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc aactccagtg gtgctgcact
     6781 ttctgatgcc gttaattaag cctttccgtg aacgctatcc ggaagtcact ttgtcgctag
     6841 tctcctccga aacgattatt aatttgatcg aaagaaaagt ggatgtcgcg atacgcgctg
     6901 gtacgttaac ggattccagc ttacgtgcca ggccgttatt taacagttat cgaaaaatta
     6961 tcgcctcccc cgattatatt tcccgctacg ggaagccaga aacgatcgac gatttaaagc
     7021 aacatatttg cctgggattc actgaacccg cttccctcaa tacctggccg atagcccgta
     7081 gcgatggaca attacatgag gtgaagtacg gtttgtcatc caatagtggg gaaacactga
     7141 aacagctttg cctgagtggc aacgggattg cgtgtttgtc cgactacatg atcgacaaag
     7201 aaatcgctcg cggagaattg gtggagttaa tggcagataa agtgttgcca gtggaaatgc
     7261 cattcagtgc agtctattac agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg
     7321 atttccttag cgagcatgta aaaacagctc ccggaggagc tgtcagagag gcttaatccc
     7381 attcaggagc cagaccttcc gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg
     7441 cttttttatc ttcggcatca agctgtaaat tttgtgcctt aagattactt tccaggtttt
     7501 cacgtttagt agaagaagga attactgagt aaccttcccc catagcccac gccagaatca
     7561 cttgtgccgg agtcgcattg tgtttagctg cgatacgagc aataacctca tctttcaggg
     7621 ccttaccata cgccagcgtc atataggaag taatatggat gccgtgctgt ttagcccaga
     7681 caaccacttt acggttttgc agataaggag agagttcaat ctggttagta gcgatgtttt
     7741 cagcgccaac agcagcaatc gttttttcca tcaacgggat cgtgaagttg gaaataccga
     7801 tctcacgtgt cagaccttgt tttttggctt ccagcagcgc ctgcataaac tcttcaacag
     7861 agacttcatc gtttggtgac ggccagtgga ttagcgtcag atcaacataa tcggtacgca
     7921 atttttgcag gctctctttc agacttggga tcaatttgtc tttgctgaga ttttcaatcc
     7981 agattttagt ggtgatgtag agttcatgac gtggcacgcc actttctgca atcgcctgac
     8041 ctactgcggc ttcgttatca tagatttgtg cggtatcaat tgcgcgataa ccaagttcaa
     8101 gcgccgtttt cacagatgaa ataacaacgt cgtctttcag acggaaagta cctaaaccaa
     8161 atgcagggat agccataata tgcctctttt aatgctattt tcgttaactg aggtgattat
     8221 gggcttcaga ctgttgcgga aaaagagcgt aaaacgcaga ggatttttgc gattctggca
     8281 ataatagaaa tgtagacgaa aaaaaacctc gagcgataac tcgaggcttc ttaata
//
LOCUS       DAZFYC010000176         8183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFYC010000176 DAZFYC010000000
VERSION     DAZFYC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.177"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..920)
                     /locus_tag="ACHKW5_004732"
     CDS             complement(<1..920)
                     /locus_tag="ACHKW5_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFO7513949.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPA"
     gene            complement(1421..4102)
                     /locus_tag="ACHKW5_004733"
     CDS             complement(1421..4102)
                     /locus_tag="ACHKW5_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO7513950.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTCP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEEIFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAET
                     YRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(4343..5389)
                     /locus_tag="ACHKW5_004734"
     CDS             complement(4343..5389)
                     /locus_tag="ACHKW5_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO7513951.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(5493..5642)
                     /locus_tag="ACHKW5_004735"
     CDS             complement(5493..5642)
                     /locus_tag="ACHKW5_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513952.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            6064..6255
                     /locus_tag="ACHKW5_004736"
     CDS             6064..6255
                     /locus_tag="ACHKW5_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7513953.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(6308..6541)
                     /locus_tag="ACHKW5_004737"
     CDS             complement(6308..6541)
                     /locus_tag="ACHKW5_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513954.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(6637..7260)
                     /locus_tag="ACHKW5_004738"
     CDS             complement(6637..7260)
                     /locus_tag="ACHKW5_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7513955.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(7349..7858)
                     /locus_tag="ACHKW5_004739"
     CDS             complement(7349..7858)
                     /locus_tag="ACHKW5_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513956.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLCSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT         2354 a         2030 c         1648 g         2151 t
ORIGIN      
        1 gctggttttg atatacgttt acgcagggca aaataggcac cgctttcatt ggcataaagc
       61 gccccgctgg cagcgcctga cacccgctca cacatcctgg cccaggtttc cggctccaga
      121 acatggtaaa gccacagccc cttacgttgt tcaggaccaa aaggttcaca gacacgcatg
      181 ttgcgtaacg gcacgtcggc acggtacatc aggtcataca gaggattgta aatggcacaa
      241 gctctggcgt tatatatcca gatatcgcgg gctttccagt catacaacgg atacatggtg
      301 taataaaacc cttcaggcga tgccgtggtc cagggcttat catcggcata tctcagtttg
      361 cgctgagaca ccagccccat aaagcgattg agggattcat ccgcacgaac accggtcagc
      421 actgccactc cacaccggtt accggcaaac caggaagaaa atgccggaac aaattcttca
      481 aacgtcatgg cataccggaa aaagggaaaa tatgtcatat ccgtaatggc ctcttccggt
      541 ggctggcgaa cccaggtcac tcctggctcc cagcatatcc actccggctg aaactgcgag
      601 acgccgttta ccgtggtcag gggaagtgcc acccagtaaa aggtttccgt cacatcatgg
      661 tacatttccc gcatcttctg aatatgttca atggtgcacc gatactgggc ctcccagtca
      721 atgaacagaa cagagaaatg acgttttctc ctgcgggcca cctctgccac aagatggaaa
      781 agcacggtgg aatcctttcc acctgaaaaa gacagacata ccgacggaaa tgtttcaaaa
      841 atccactcaa tgcgggcgat tgcagcacgc aaaacatttt ctccagtaag gattttttcg
      901 gttaaaagcg tatcactcat tttcctggct ttatgtcgaa taattctgtt tatacattca
      961 ggcaaacttc attaataact caccgctttg gggataaata ataacaacaa aaatcttcag
     1021 atattaacct ggttgttgga tttgttatca catcctttat ccacctgctt cttactggta
     1081 acacacctgg aaaatccatg cattttcaac atatactatc ctttcaactt gtaaataaga
     1141 aaatatgatg cagatggtga acaattcaaa tatcattttg caggaagtat tcactgtaat
     1201 agaattaccc cctgataaag tttattcact aaggaattat tcaacagaaa cattccctgt
     1261 tagctttgag caatagcact tatttttata tgtatcagga atacgtacgt tgatgtaacc
     1321 aaaacaaaga agcctgtgtc catggggttc gtacaattgt gtgtggcaca tgaccaaatc
     1381 aggtttgaga cctcccgctg caatattttt tattcagatt ttagcctgtc aggcctgaca
     1441 gaaacgctcc caacttccct tctatatcac tgtctatagt acgtaatctt agcaatggca
     1501 ggccgcattt cttcagtata ctgtttttaa gttcatcacg ttcagcctga acactggtca
     1561 gatgataacc tccatccact tcaataaccc ctaatggagt cttcccgacc ttatagtaaa
     1621 caacaaagtc acaactggcc cggttacgca tgaataactg ttcacggtgt gtaaaatccc
     1681 cccgctccag caacaccaac tgattcagcg ctatttgtga atgaaacatc atactccgat
     1741 aactatcctg cgacaggata tcccgcaaca ggcaggccac aatctgttct gacttaaagt
     1801 gtgaatcatt tgaattcaat ctactattca gtcgttccag tgatttgtca tactcactgt
     1861 acagcaaatc aaatgctgat atgaccgggc tctcgaaaat ctctccgtca tcagcgtagt
     1921 atttgatgta acgaatcagc gccgcaatat gtccagcgtg gcgttcaaat acatcattac
     1981 cagtcaccag cgtaaattta tttcgggccc ttgaaacagc gacattcacc agttcaggat
     2041 tatcaacaaa cgcgatgttc cgactgtgct ggctgctgcg cttcttatcc agcacagtgg
     2101 aaaacacaat ttcatcgcac tcccgtccct gaaatttatg caccgtcgat ttcacaaaat
     2161 cagcaggcag cactttttca gccagattaa cctgtgcatt atatggggca atgtatcccc
     2221 tgctactttc ttcatcccag taatgacctt ccagagattc aagctccctg agatttgaaa
     2281 aattacgggt atggtttcct tttgccgtga tgaccagaga taatgaggct tcaccggaat
     2341 caaccgttaa cggtatcaga gcattatcat aaaactgctt attacaaaat tgaatgattt
     2401 taggatgaca acgataatgc tctttaagaa gggttactgg caccatgttc ctgaacaaca
     2461 tacaaaccga atcaagtaga ctatatttct cacaattata gtattctgcc ggtggagatg
     2521 ggctgttcgg gagtaataca ggaacatgtg gaagttgctt acgatcccct acaacgatta
     2581 cattacgggc acatcccagt cccagaatgc ccggaacaat atcctgctgc gaggcttcat
     2641 caataataac ataatcaaga agggccccct tgccgataga gttaataatc gaatgcgtac
     2701 tgctaccaat aatcggaaaa cgctttataa aacggtcaaa ttcatccctg tatgtctctg
     2761 ccgagaatga acttcttgtt gaaacatttc ttcgcagatg ttgctttaaa taaagcatag
     2821 accatgatgt caggcgcccc agtaaaattt taaaatcaga ttttttaaat acttcctcat
     2881 attcattcag agaaatttgt ttttcccgga tatttttttc ataataagaa agctgaagag
     2941 aagtaaatag cgccagcctt ctctccggaa tcatcaatgg tttgactttc agtatcatga
     3001 agtttaacaa caaatcaatt ctgtttctga atccaatccg cctgtcagaa aggtaatgaa
     3061 tataagccat caggtcggtt gttttttgtg aggaaaatcg gtatttgtga gaaaaaatct
     3121 cctccggtcg aagattctga ctctgccatt gctgcaggta tttatactca atattcagct
     3181 cattaatttc tgcttttaac gaagcgacct ggttaatggc attcaggtgt tttttcactt
     3241 gttgaagtac atcatcaatc gcatttgcag acggtgagtc aggtaagact tcctcagaac
     3301 gcgaaatcga cgttgaaaaa aactgttgcc tgttctccgt actccccagt cgtgccatca
     3361 catatcccag ttgctgtttg tccatctttt cataaacatt actgacagct gagttattat
     3421 tggacaaaat tgccactgtt ttattctgaa ttaatatatt agcaacaata ttgagtatag
     3481 tctgtgtttt acctgttcca ggtggtcctt caataactga aatctgtgag gaaaatgccc
     3541 gttcgacagc cagcaactga ctttcattaa gaccaaacgg aaaaatcaaa ccccatggcg
     3601 aatcacgttt tttactctgt cctgaacagt atgcatgcaa ggcggtatcc ggatgaggaa
     3661 ctattttctt tatctgccta aggatattct catcgatatt tctgttatta ccacgggcat
     3721 aaaaaatacg ttcattagcc accctacaca agtaattaaa cacggcatgc tctgtaaatg
     3781 ctgaacactg ttgaaagtca aggccggtcg ttttcatgat gtagggtcgt tcaccctcgc
     3841 ggtaataaac agcagcatat ttatcaccat atataataac cagttcagcc cgggatgtta
     3901 cagcgttccc tttcctttcg aggaaaacat ttcgcaaatt tacagttggc gttatctgac
     3961 atacagaaag cggacaagta tattttctcc cgctgcgaaa atgacaggtc agcataacct
     4021 ctctgtcgtc atttccagcc cacagcgtcc agtcggtaat tttatgagtc ttattttcac
     4081 cgtcaacaca aatcgtgacc atattcttac tcagaaatta tcatttaact tacggaaaat
     4141 tgttaaggca ctcccgatat tagctccgga ggattttcca caactccggg actgttaaca
     4201 tatttctgcc tgccatatat acattgaatt tagtcataac agtcaatacc agaattacag
     4261 gaatgcgatg ccgatacgct ggttattgaa tactggtagc ccctgagcac taattcatca
     4321 cgatactctg tatttactgt tttcactgct ttttacaacc attcccccag gtaaaatgct
     4381 gctcctccgg tgatggctgt gtcagaaact gccatcccgg aatagccacg ctgaccgcca
     4441 gcaatgccgc acagacacca gccacaaacg gtttcagaaa cgtgggcggg gatttctgaa
     4501 gggagggtga ctgctccgcc tgcactttcc ccttgcgatt atcaaggctc agtgcatcca
     4561 gacgcaccgc gccatttgcc gcaagcagcc cttcccggtt atccacgctt cccggcagac
     4621 tcagcgaaaa atcccctgtt cccgtctgaa tcagactgcc tttacgattt gacaggcttt
     4681 gtgccgatat ctgcagtgtg tcggcattta tcgtaccgcc attactgctg aactgcccgg
     4741 cagtccgggc ggaaagcgta tcggcactca gttgcgcatt ctgtgtagac agatttccac
     4801 tggttgcatt cagctcagta tgttgtccct gtatccggct gttacctaaa tccagcccct
     4861 gctcaatgtc ccgtaaatcc agcagagaac cttcgtgtgc cgggaatgag gaatacaact
     4921 gaatatagtc atcactgtcc ggtgtcagac gcacatgtcg cacaacaccg ggaataataa
     4981 ccagccggag gataccgctt tttaaatcct gcgaaggtgc cagaacacgg gtggtgacat
     5041 aaccatgatc gaccagacgg ttctgcaatg tactcatcag cagattaatt cctttcgccc
     5101 ccaggcaatg cccgaccgcc ccgttggcga ttttttgtaa aggcagccag tgtggtaacg
     5161 catcagcccc cttcagttcc gtctgtttaa tctgaaaaca gggcgtttca acaggaaaat
     5221 taatcttatg ggcaaaagag ccaggtgcag aaaggcgaac atcaggggct gaaggcgtca
     5281 gttgttgctc aagggcttcc tgacgctgct gctgaatgat aaatgcttca tctccgctac
     5341 gccggacatc cgctgcctga agagataaag gcgataataa agcagacatc agtgtcagtg
     5401 acagcaagga cggacagcgc cggaataagc tttgttcctc agtcctctcg ttcctgcaac
     5461 ataattttcc agtcaaacga aaaacctttt attcatgtca taccaacaat caccagcctc
     5521 ctgacgatac ctcccaccat gttttgcgat ggcacgctac cgcccttccg gtatttaccc
     5581 gcatcaaccg gtaccagaat gctgagtaac aaaccagcta gaattgactt aattttgatc
     5641 attatagaat aatgatctga actacagcaa catgacagat ttatcgatcg atataacaca
     5701 aatgaatagt tctttattct gacaaatatc gcaaaaacag cacattacaa aatttataac
     5761 cataattact ggaaaagtgc agaaaattat tccactgaat gcttttaata cagttggtta
     5821 gcttaaaaaa tagcaatttt tgatttcagt cattgaaagt tgcaatgttt gacgattcaa
     5881 cagcatttaa gtgagtttca ggatatggga aggacatgaa aggaggagcg caataaaata
     5941 cacaggaaga aggggaaaat gtttcgtaaa aacagaaaat taacagcgca catcaggaaa
     6001 cgcagtctcc tgattcagtg ctaccacatg aaaatttagt taactgttac cgtactaaaa
     6061 tttatgtcag aagatgttcc attaccgaaa gtcaatcagc gatacaaaga cgatcatggt
     6121 gcgcttgtca ccctgaccag tgttgaggaa acccgggtgg tgtttatgca ggacggctat
     6181 ccgcatccct gcatgcgacc gatgtacaac tttctgggaa aattcaaacc cgaaccccgg
     6241 gaagaaacag aataaaacga aatccctgcc ggcaatcaca ctacctgata gcagcataat
     6301 ccacttatta attctcatcc ccggagacaa gcccagaaaa ccactccggg tgttcactgt
     6361 atgccgtgtt cagcgcatcc ctggaaacgt ttatataaaa gctgtcctgc aatttgcggg
     6421 taacaacttc tgccaggcga ttccggttat ccaccagtcc ggcataacct ctctctgcca
     6481 gccactcatt cagttcatgt tccagagaca gattgataat atcataatcg gaccttgcca
     6541 ttcccgtttc cttagcacat gttttcaggc acggggataa agtataaaga atgtcatact
     6601 gctgctgcca gagcaatatt ccccctgatg gttgtatcag agatggtatg ccggtacttt
     6661 gcgggttgtt cccgtcaggt tatcgtgcac ctccacggtc gtggtcacca ccggcattcc
     6721 ggcctttctc agcctcagaa catcagccgc aatacgctga ctgccaaacc agaacaggcc
     6781 gtccagtgca gtcaccagta aacccgcctc cagtgcatac ttcagccgtt cacggggcgc
     6841 tttcacttcc cgggcaacct gctgatatgg caatgatgtg ttttcattcc caaccacgcg
     6901 acgaatacgg tgagacagat gataccggta tgtatccggc acaccagaaa ggctggcctt
     6961 caggctgtac acgcagccaa accgtttatc gttaaacacc acatttttct ggctgatgcc
     7021 ccattcttca cgcagcgcgg caatcaattg cggtgtacgg gtaagcagca agcgaaaagg
     7081 cagttcaaaa ctggtgacgt aatccacatt cagcagtgca atacgcaacc gttcttctgg
     7141 cccggcctct gtctgccggc actcctccag aacatcctgc cactgccggc gaagacgggg
     7201 ggattcgttc cgttctgtaa aacagtattt atccgccaga tagtcaattc gtgtacgcat
     7261 actgaaagga attccgtata aagattcagc agacaaaact ttatcagcca gtaaaaatta
     7321 acggaagcat tgatattcct cccgacaatc accggatgat tgttgcaata acctgcgaca
     7381 taaaggactg aacagccgtt tttaacgcca cgtattgccc tgatgaacca ggccgggcca
     7441 cccgaaaaca tctttccacc cggcattgtc cgccagcact tatcaccgac caggtctgtt
     7501 gcagtaaatc caccagttgc gcacattctt catcagccat gacgccgact cgcggatggc
     7561 aaatactcgt ctggctgagc agcaacagac atcgtatctg tgctccttca tgttgttccc
     7621 gcaaccagcg taataccacc ggcgctgatg ggctgaccgt gtgattatgc tccgtaatac
     7681 gttcgtgtac ccgtcggtga aaggaactac aaaatgtctg aatctgttga ccatagattc
     7741 cttctgccga acagagcgtg aagtgaaatg ccaacagatt tggccagtgg tccaccgcct
     7801 gctgaacaaa acgccggatt tcccccggct ctgaaattaa ggcttcggtt atttgcacga
     7861 aatgttcctt ttcaacccct gttgaatttt gtcaagccgg tgcagacaca gtaaagcgcg
     7921 catcaacacc tgaaaggtac gatacaaaca caagttacaa acaataagaa cggaaggaca
     7981 attaatcagt gaggaataat aactaaatta accaaaagaa tgtaactgac aatactacca
     8041 atacttaaat agcaatatat taattaatgt aagaacagat gcccggtagg acatatacac
     8101 aatacatcca caaacacact cttttcatta aatcaatcac ctgattaaac catgaaagtg
     8161 tgatgtaagt caaaattact gat
//
LOCUS       DAZFYC010000177         8086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFYC010000177 DAZFYC010000000
VERSION     DAZFYC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.178"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1258
                     /locus_tag="ACHKW5_004740"
                     /pseudo
     CDS             1..1258
                     /locus_tag="ACHKW5_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1227..2963)
                     /gene="aceK"
                     /locus_tag="ACHKW5_004741"
     CDS             complement(1227..2963)
                     /gene="aceK"
                     /locus_tag="ACHKW5_004741"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO7513957.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3244..4548)
                     /gene="aceA"
                     /locus_tag="ACHKW5_004742"
     CDS             complement(3244..4548)
                     /gene="aceA"
                     /locus_tag="ACHKW5_004742"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO7513958.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4578..6179)
                     /gene="aceB"
                     /locus_tag="ACHKW5_004743"
     CDS             complement(4578..6179)
                     /gene="aceB"
                     /locus_tag="ACHKW5_004743"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO7513959.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLASCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6448..7377)
                     /gene="metA"
                     /locus_tag="ACHKW5_004744"
     CDS             complement(6448..7377)
                     /gene="metA"
                     /locus_tag="ACHKW5_004744"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO7513960.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7534..7977
                     /locus_tag="ACHKW5_004745"
     CDS             7534..7977
                     /locus_tag="ACHKW5_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7513961.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2046 a         2020 c         2016 g         2004 t
ORIGIN      
        1 acctaatgga aattttacct caacgctcag acactcttga taaacaagaa ttaaacctaa
       61 aatcagatag aaaagaaaaa gaattcccaa gaattaaact taatggtcaa tgttattttc
      121 cggggcgacc ccaaaaccgt atagtatgcc gacacattgc tgcacaatat attaatgata
      181 tttatcagaa tgttgattac aaaccccatc aagatgatta ctcttcagct gaaaaatttc
      241 tcacgcactt caacaaaaaa tgcaaaaacc agactttggc gttggtttcc agccgtcctg
      301 aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca tattttgaca
      361 agatggaatc aaatggcatc agtgttatgg cagccatatt actggtggat aaccatgctt
      421 tgacggtccg gctaagaata aagaacacaa ctgaaggatg tacccattac gtggtttcgg
      481 tttatgatcc taatgtaact aacgataaaa taagaattat gagcgaaagc aaagaggata
      541 ttaaacacta ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa
      601 atgatcatgt tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat
      661 tgatgttaaa aggatctgtg gatgaaataa cccctccatt atcaccagca acgatgaatt
      721 tgctaatggc aattggtcag aatcaccaac ttacgcaact gatgattcaa ctccagaaaa
      781 tgccagaact acatagaaca gaaatgttga ctgcctataa tagtggacat atgaacgtta
      841 ttaatactat ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa
      901 tatgaagccc ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat
      961 acagcataaa caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg
     1021 cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa
     1081 atactctgcc tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct
     1141 taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc
     1201 ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat
     1261 ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt
     1321 agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca
     1381 atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag
     1441 acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg
     1501 cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg
     1561 tgacgggtga caccgaagtt tttaaacagc atgtcgccag gaaaaatgtt agcagcggca
     1621 agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact
     1681 tgttccagcc agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca
     1741 atctgctcac caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat
     1801 gccggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt
     1861 cggcccacgc gatcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac
     1921 atctctttct gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc
     1981 agcgtaaaca ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta
     2041 cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag
     2101 ccgatagcca tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg
     2161 accagcgctg cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca
     2221 atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc
     2281 tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc
     2341 caggcggctt tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg
     2401 ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta
     2461 ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag
     2521 ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct
     2581 ggctgagagc taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga
     2641 cagtacacgg agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc
     2701 cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta
     2761 atgcagcgca gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt
     2821 ttcatcgcct gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa
     2881 gtcacttcga ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc
     2941 agtaattcca ggccacgcgg catttagcgc cctcatcagg agcagagaat tgccggatgc
     3001 ggcgtaaacg ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca
     3061 agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc
     3121 tacgttcggc aacggctgta ggcatgataa gacgcgcaag cgtcgcatca ggcatcgtgc
     3181 accgattgcc ggatgcggcg tgaacgcctt atccggccta cagtcagcaa cggttgttgt
     3241 tgcttagaac tgcgattctt cagtggagcc ggtcagcgcg gtgactgaag acgtgccgcc
     3301 ctgaataata gtcgtcactt tatcgaagta acctgtaccc acttcctgct ggtgagatac
     3361 gaaggtatag ccatctttcg cggcggcaaa ttccggctgc tgcactttct caacgtagtg
     3421 cttcataccc tcgccctggg cataggcgtt tgccaggtca aacatgttga accacatgct
     3481 gtggatacct gccagggtga tgaactggaa cttgtagccc atatccgaca gctgctgctg
     3541 gaagctggca atagttttgt cgtcgaggtt tttctgccag ttgaacgacg gcgagcagtt
     3601 ataagccagc agtttgcccg gatatttcgc gtggatagct tgtgcaaagc gacgcgccag
     3661 ttccagatcc ggcgtggagg tttcacacca gaccaggtca gcatatggcg catacgccag
     3721 gccacggctg atcgcttgct caatgcccgc atgagtacgg aagaagcctt cactggtacg
     3781 ctcgccagta ataaattcac tgtcatacgg gtcgcaatcg gaggtaatca gatccgccgc
     3841 atcggcatcg gtacgagcaa ccagcagggt tggaacgccc gtcacgtcag ctgcaagacg
     3901 cgccgcgacc agtttctgaa tagcttcctg agttggcacc aaaactttgc cgcccatgtg
     3961 accgcatttc ttcactgacg ccagctgatc ttcgaagtga actgccgctg caccggcttc
     4021 aatcatcgct ttcatcagtt caaaggcatt caggacaccg ccaaaaccgg cttccgcatc
     4081 ggcaacgatc ggcaggaagt aatcgacata gcgcggatcg cccggctcaa tgcctgcgga
     4141 ccattggatc tgatcggcac gacggaaggt gttgttgatc cgctccacca cagctggcac
     4201 cgagtttgcc ggatagagcg actgatccgg atacatgctg gccgccaggt tagcgtccgc
     4261 cgctacctgc catcccgaca gatagactgc ttcaataccc gctttcgcct gttgcagcgc
     4321 ctgaccgcca gtcagtgcac cgaggctgtt gatgtagcct tttttcgact cgccatgcag
     4381 cagacgccac attttcgccg cgcccagttg cgccagggtg cattcaggat tgactgaacc
     4441 gcgtaatttc accacatctt ccgcgctgta tgggcgagta atgccttccc aacgcggttg
     4501 agtccactct ttctgtaatt cttcaatttg ttgtgtacgg gttttcatgt gcagatgctc
     4561 catagttatg tggtggttta cgctaacagg cggtagcctg gcagggtcag gaaatcaatt
     4621 aactcatcgg aagtggtgat ctgttccatc aagcgtgcgg catcgtcaaa acgcccctgg
     4681 gagaaacgtt cttcgcccag ttcgctggca atgactttca tctcttcacc cagcatctgg
     4741 cggaacaagg ctttggtcac cggtttgcca ttgctcaacg ttttttgatg atggatccac
     4801 tgccagattg aggtacggga aatttcagcc gtcgccgcat cttccatcag gccataaatc
     4861 ggcacgcagc cgttgccgga gatccatgct tcgatgtact gcactgccac gcgaatgttg
     4921 gcgcgcatac cttcttcggt gcgttcacca tcacaagatg ccagcagctg atcggcagta
     4981 atcggcacgt cttgttcgcg catcacttca agctgatttt tacgggagcc gagaatgtcg
     5041 ttgaataccg ccattgccgt atccgcaagg cctgggtgag caatccatgt gccatcgtga
     5101 ccgttattgg cttccagcgc tttatccgct tttactttgt tgagcacctg gttattgcgc
     5161 tcttcatctt tgctcggaat aaacgctgcc atgccgccca tcgcaaaagc accgcgttta
     5221 tggcaggttt taatcaacag gcgtgagtaa gcattcagga atggtttatc catcgtcact
     5281 gcctgtctgt ctggcaggac gcgatcggga tagtttttca acgttttgat atagctgaag
     5341 atgtaatccc aacgaccgca gttcagacca acaatatggt cacgcagcgc gtgaaggatt
     5401 tcatccatct ggaacacggc gggcagcgtt tcaatcagca acgtcgcctt gatggtgccg
     5461 cgcggcagat taaagcgatc ttctgcatag ctgaagactt cgctccacca ggccgcttcc
     5521 tgccaggact gggttttcgg cagatagaaa tagggaccgc tgccctttgc caacagtgcc
     5581 tgatagttgt ggaagaaata gagcgcaaaa tcaaacaggc taccggggat tgcctcacca
     5641 cgccaggtga catgtttttc cggcaagtgc agaccgcgta cccgacaaat caaaaccgct
     5701 ggattgggct tgagctgata aattttgcct gcttcattgg tatagctgat ggtgccgtta
     5761 accgcatcgc gcaggttaat ttgcccgtcg atcactttgt tccagtctgg tgccagtgaa
     5821 tcttcgaaat cggccataaa gactttcaca ttggcgttca gtgcgttgat caccatcttg
     5881 cgctcaaccg ggccagttat ctcgactcga cgatcttgta agtccgcggg aataccacga
     5941 attttccagt ccgtatcgcg aatggaagct gtttccgaaa taaaatcagg caaagttccg
     6001 ttatcaatat cttgctgctg ctgaatgcgc gctgccagaa gtttattgcg ttgtggcgta
     6061 aaatgcgtca ccagctcagt cagaaattct accgcttcgg cagtaagaat ttgcttctcc
     6121 tgctcgccat acggccttat gaaagccagt tcatcggttg ttgttgcctg ttcagtcatc
     6181 gtgcagctcc tcgtcatgga tccgaaaact tccccactgt gaacgaagga tcgttgtgcg
     6241 cttttcgttc agcacaacta agactactca tttaaaatgc aaaatcaaaa acaatttcca
     6301 ttttaattaa aaatacttga taacttattg ataacaatat gaataaaatt tattcacctc
     6361 ctgaggtgcg tttcggaaat aaaaaaggca cccgaaggtg cctgaggtaa ggtgctgaat
     6421 cgcttaacga tcgactatca cagaagatta atccagcgtt ggattcatgt gccgtagatc
     6481 gtatggcgtg atctgataga cgtaatagtt gagccagttg gtaaacagca aattaccgtg
     6541 actacgccag ctcgctcgcg gtgtattttg cggatcattg tgcgggaaat agttatacgg
     6601 tacttccggg cctagtccgg cttccacatc gcggaaatat tcctgcgcca gcgtttgcgc
     6661 atcatattcg ggatggcccg tcacaaaggc aatgcgctta tctttgctgg caaacagata
     6721 tgcatcccct tcttccgtct ctgccagaat ttccagatcg gtgtaatcac gaatcaacgc
     6781 tgccggaaag tcagcatagc gcgaatgtgg tgccaggaat gaatcatcaa agccacgcgt
     6841 cagaagcgca tgaggatgga gaatatgatg ctcgtaaacg ccagagagtt tgtcggtgcg
     6901 agtttgctta ggaatgccgt agaggatatt gagcgcggcc tgtaccgccc agcagacaaa
     6961 cagcgtcgag gtgacgtgat ctttcgacca ctccagcacc tgtttgatct gcggccagta
     7021 agcgacatca ttaaactcca ccaggcccag cggcgcaccg gttacaatca gaccgtcaaa
     7081 gttctgttcc tgaatatctt caaagttaca gtagaagttg ttcagatgct ctgcgggcgt
     7141 gttgcgcgat tcacgggaat cgatgcgcaa cagctgaata tcgacctgca aaggtgagtt
     7201 tgaaagcagg cgcagaaact gattttcagt ttcaatcttc ttcggcatca ggttaaggat
     7261 cagaacctta agtggacgaa tttcctgacc agacgcacga gaagttgtca tcacaaagac
     7321 gttttcttca cgcaagaaat tgacggcggg tagctcgtcc ggcacacgaa taggcataac
     7381 ctgattacct cactacatac gcttatacgt ttagacatcc agatagctga agataaccag
     7441 aaaatttgcc aatgtcgagc ctgcatgttg aaggtgagaa agtttcaagg aaaaggctac
     7501 gttagaatat aagaatgacg aaaaggagag aaaatggtta ttagtattcg ccgctcacag
     7561 catgaggaag gggaggaact cgttgcgatt tggtgtcgat ctgtcgatgc cacccacgat
     7621 tttttatcag cagagtatcg ggccgagctg gaagagctgg ttcgttcctt tctgccggaa
     7681 gcaccgttgt gggtcgcggt taatgaacgg gatcagccgg ttgggtttat gttgttaagt
     7741 gggcagcata tggatgcgct gtttatcgat cctgatgtgc gcggctgcgg cgtgggtcgg
     7801 atgctggtgg agcatgcgct ctcgatggca ccggaactga caaccaacgt taatgagcaa
     7861 aatgagcagg cggttgggtt ctataagaag gtgggtttta aggttacggg acgctctgag
     7921 gtggacgatt tggggaaacc gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc
     7981 gcccctgccg tgcctgccca ggctggagaa gacgcgccag cgtcgcatcc gacctttttc
     8041 tgcaccagcg caaaaaggcc cagtcttccg actgggcctt tcgttt
//
LOCUS       DAZFYC010000178         7876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFYC010000178 DAZFYC010000000
VERSION     DAZFYC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.179"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            305..1621
                     /gene="ugpB"
                     /locus_tag="ACHKW5_004746"
     CDS             305..1621
                     /gene="ugpB"
                     /locus_tag="ACHKW5_004746"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO7513962.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            1719..2606
                     /gene="ugpA"
                     /locus_tag="ACHKW5_004747"
     CDS             1719..2606
                     /gene="ugpA"
                     /locus_tag="ACHKW5_004747"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO7513963.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            2603..3448
                     /gene="ugpE"
                     /locus_tag="ACHKW5_004748"
     CDS             2603..3448
                     /gene="ugpE"
                     /locus_tag="ACHKW5_004748"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO7513964.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            3450..4520
                     /gene="ugpC"
                     /locus_tag="ACHKW5_004749"
     CDS             3450..4520
                     /gene="ugpC"
                     /locus_tag="ACHKW5_004749"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO7513965.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKCRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            4517..5260
                     /gene="ugpQ"
                     /locus_tag="ACHKW5_004750"
     CDS             4517..5260
                     /gene="ugpQ"
                     /locus_tag="ACHKW5_004750"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7513966.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(5247..5687)
                     /gene="yhhA"
                     /locus_tag="ACHKW5_004751"
     CDS             complement(5247..5687)
                     /gene="yhhA"
                     /locus_tag="ACHKW5_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO7513967.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            5807..7549
                     /gene="ggt"
                     /locus_tag="ACHKW5_004752"
     CDS             5807..7549
                     /gene="ggt"
                     /locus_tag="ACHKW5_004752"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO7513968.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAINFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(7587..7871)
                     /gene="yrhB"
                     /locus_tag="ACHKW5_004753"
     CDS             complement(7587..7871)
                     /gene="yrhB"
                     /locus_tag="ACHKW5_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO7513969.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         1778 a         2030 c         2232 g         1836 t
ORIGIN      
        1 gggtgagttg caatatattg aatttgcacg atcttgtagg ccggataagg cgtttacgcc
       61 gcatccggca tgaaacgatg agtaatctgt agagtttgat tcagaccttc tattttccgc
      121 ttatccgtgc cccatctccc attttccctc acccacgccg tcaccgcctt gtcatctttc
      181 tgacacctta ctatcttaca aatgtaacaa aaaagttatt tttctgtaat tcgagcatgt
      241 catgttaccc cgcgagcata aaacgcgtga attcgcgcat tcggtacaac aagagagata
      301 aacgatgaaa ccgttacatt atacagcttc agcactggcg ctcggactgg cgttaatggg
      361 gaatgcacag gcagtgacga ccattccgtt ctggcattct atggaagggg aactgggtaa
      421 agaggtggat tctctggccc aacgttttaa cgccgaaaat ccggattaca aaattgtacc
      481 gacctataaa ggcaactacg aacagaattt aagcgcgggg attgccgcat ttcgtaccgg
      541 caacgctccg gctattttgc aggtttatga agttggcacc gccaccatga tggcatcgaa
      601 agccattaaa ccggtatatg acgtgtttaa agaggcgggg attcaattcg atgagtcgca
      661 gtttgtgccg acggtttccg gttactactc cgacagcaaa acgggccact tactctccca
      721 gccattcaac agctcgaccc ccgttctcta ttacaacaaa gacgccttca agaaagcagg
      781 attagacccg gaacaaccgc cgaaaacctg gcaggatctg gcggactatg ccgcgaaact
      841 gaaagcctcc ggtatgaagt gcggctacgc cagcggctgg cagggctgga tccaactgga
      901 aaactttagc gcctggaacg gtctgccgtt tgccagcaaa aacaacggct ttgacggcac
      961 ggacgcggtg ctggagttca acaagccgga gcaggtgaaa cacatcgcca tgctcgagga
     1021 gatgaacaag aagggcgact tcagctacgt cggtcgtaag gatgaatcca ccgagaagtt
     1081 ctataacggt gattgcgcga tgaccaccgc ctcttccggt tctctcgcta atattcgtga
     1141 gtacgccaaa tttaactatg gcgtaggcat gatgccttac gacgccgatg cgaaagatgc
     1201 accgcaaaac gccattatcg gcggggccag cctgtgggtg atgcagggca aaaataaaga
     1261 aacgtatacc ggcgtggcga agttcctcga cttcctcgcg aagccagaaa acgctgccga
     1321 gtggcatcag aaaaccggtt atctgccaat caccaaagca gcgtatgacc tgacccgtga
     1381 gcagggcttt tacgagaaaa acccaggggc ggataccgcg acgcgtcaga tgctgaataa
     1441 gccgccgttg ccgttcacca aagggctgcg tctgggcaac atgccgcaga tccgcgtgat
     1501 tgtggatgaa gagctggaga gcgtgtggac cggtaagaag acaccacagc aggcactgga
     1561 taccgccgtt gagcgtggaa atcagttgct gcgccgcttt gagaaatcga cgaagtctta
     1621 atcagtgtaa tgtcggatgc gtttcgctta tctgacctgg catcgcgtgt aggccggata
     1681 agcgaagcgc atccggcaca gttcaggaat taaccgtaat gtcatcatcc cgtccggtgt
     1741 tccgctcgcg ctggctgcct tatctgctgg tcgcgccgca gctcatcatc accgttatct
     1801 tttttatctg gcctgcgggc gaagcgttgt ggtactcgct acaaagcgtc gatccgtttg
     1861 gtttctccag ccagtttgtc ggcctggata acttcgtcac gctgttccat gacagctact
     1921 atctcgactc cttctggacg acgataaaat tcagcacctt tgtcaccgtc agcggtttgc
     1981 tggtatcgct gttctttgcg gcgctggtgg agtacatcgt gcgcggcagc cgtttctatc
     2041 aaaccttaat gttgctgcct tacgccgtgg ctcccgccgt tgccgccgta ttgtggatct
     2101 tcctgtttaa ccccggtcgc gggctgatca cccattttct cgcggagttc ggctacgact
     2161 ggaaccacgc gcaaaacagc ggtcaggcaa tgtttctggt ggtgtttgcc tcagtatgga
     2221 agcaaatcag ctacaacttc ctgttcttct atgccgcgct gcaatccatt ccccgttcgt
     2281 tgatcgaagc cgcagccatc gacggtgcag ggccgattcg ccgcttcttt aagattgcgc
     2341 tgccgcttat cgccccggtg agtttcttcc tgctggtagt gaacctggtg tatgccttct
     2401 tcgacacctt cccggtgatc gacgccgcca cgtccggcgg gccggttcag gccaccacga
     2461 cgctgattta taagatctac cgcgaaggtt ttaccggact ggatctggct tcgtctgccg
     2521 cacagtcggt ggtgttgatg ttcctcgtca tcgtgctgac ggtggtgcag ttccgctatg
     2581 ttgaaagcaa ggtgcgttac caatgattga gaaccgtccg tggctgacga tattcagcca
     2641 taccatgctg atcctcggga tcgcggtgat cctcttcccg ctgtacgtgg cgtttgtcgc
     2701 ggcgacgctg gataaacagg ccgtctatgc cgcgccgatg acgctcatcc ccggcacaca
     2761 tctgctggaa aacatccaca acatctgggt gaacggggta ggcacgaata gcgcgccgtt
     2821 ctggcggatg ttgcttaaca gctttgtgat ggcgttcagc attacgctcg gcaaaattac
     2881 cgtctcgatg ctctcggcat ttgccattgt ctggtttcgt tttccgctac gtaacctctt
     2941 cttctggatg atttttatca ccctgatgct gccggttgaa gtacgtatct tcccgacggt
     3001 ggaagtcatc gccaacctgc agatgctcga cagctacgcc ggtttaacgc tgccgctgat
     3061 ggcctcggcg accgctactt tcctgttccg ccagttcttt atgacgctgc cggatgagct
     3121 ggtggaagcc gcgcggatcg acggcgcatc gccaatgcgc ttcttttgcg acatcgtttt
     3181 tccgctctcc aaaactaatc tggcggcgct gtttgtgatc acctttatct acggctggaa
     3241 tcagtatttg tggccgttgt tgattattac cgatgtggat ctcggcacca ccgtggcagg
     3301 gatcaaaggg atgatcgcta caggcgaagg caccacggaa tggaactcag tgatggcggc
     3361 gatgttgtta acgcttatcc ctccggtggt gattgtttta gtgatgcagc gtgccttcgt
     3421 gcgcggcctg gtcgatagtg agaaataaga tggcaggact gaaattacag gcagtaacca
     3481 aaagctggga tggtaaaacc caggtcatta aaccgctgac ccttgatgtg gcggatggcg
     3541 aatttatcgt gatggtcggg ccgtctggct gcgggaaatc gacgctgctg cgcatggttg
     3601 ccgggctgga gcgggtgaca gaaggcgata tctggatcaa cgaccagcgc gtgactgaaa
     3661 tggagccaaa agatcgcggg attgcgatgg tgttccagaa ctacgcgctt tatccgcata
     3721 tgagtgtcga agaaaacatg gcgtgggggc tgaaaattcg cggcatgggc aagcagcaaa
     3781 ttgccgagcg cgttaaagaa gcggcgcgca ttctggagct ggacggtctg ctcaaatgtc
     3841 gcccgcgcga gctttccggc ggtcagcgcc agcgtgtggc gatgggccgc gcgattgtgc
     3901 gcgatccggc ggtgttcctg tttgatgagc cgctctctaa cctcgatgcc aagctgcgcg
     3961 tgcagatgcg tcttgaactg caacagttgc accgtcgcct gaaaacgact tcactctacg
     4021 ttactcacga tcaggttgaa gcgatgacgc tcgcccagcg agtaatggtg atgaacggcg
     4081 gcgttgccga acagattggc acaccagttg aagtctacga aaagcccgcc agcctgtttg
     4141 tagcgagttt tatcggcagt ccggcgatga acctgctgac aggccgcgtg aataccgaag
     4201 gcacgcattt cgaactggac ggcggtatcg tgctaccgct aaacggtggc taccgtcagt
     4261 atgccgggcg taaaatgact ctcggcattc gcccggaaca tattgcgcta agctcgcaag
     4321 cagaaggtgg cgtaccgctg gtgatggata cgctggagat cctcggcgca gataacctgg
     4381 cgcacggacg ctggggcgag cagaagctgg tggtgcgact ggcgcatcag gagcgcccga
     4441 cggcaggcag cacgctgtgg ctgcatctgg cggaaaatca gctgcatctt tttgatggtg
     4501 aaacaggaca acgagtatga gtaactggcc ttatccccgc atcgtcgctc atcgtggcgg
     4561 cggtaagctg gcccccgaaa acaccctggc ggcaatcgac gttggggcaa aatacggtca
     4621 taagatgatc gaatttgacg cgaagttatc gaaagatggc gagatcttcc tgctccatga
     4681 cgacaatctc gaacgcacca gcaacggctg gggcgtcgcg ggtgaactga actggcagga
     4741 tttactgcgc gtggatgcgg gcagttggta cagcaaagcg tttaaaggtg agccgctgcc
     4801 gttgctttcg caggtggcgg aacgctgtcg cgaacacggg atgatggcga atatcgaaat
     4861 caaacccacc actggcactg gaccattaac gggcaaaatg gtggcgctgg cggcacgcca
     4921 actgtgggcc ggtatgacgc cgccgctgct gtcatcgttt gagattgatg ctttagaagc
     4981 tgcacagctg gcggcaccgg aactgccgcg tggtttattg cttgatgagt ggcgcgacga
     5041 ctggcgcgaa ctgaccgcgc ggctgggctg cgtctctatt catctcaatc ataagttact
     5101 cgataaagtg cgagtgatgc agttgaaaga cgccggactg cggattctgg tttataccgt
     5161 caataaaccc cagcgcgcgg cagagttgct gcgctggggc gtggattgca tctgtaccga
     5221 tgcgattgac gtgattggtc cgaactttac ggcccaatag ttttcaacgg aatgtcaggc
     5281 tgcggcgtgc tgggctgatt cagcatgtcg ccgtttctcc tctccggcag catatgctgc
     5341 tgattcaacg aactatccgg attacggttg ctgtttaaca tcccgccgtt ggtgttgggc
     5401 agcatttgct gccgggcggg atttcgctcc cccggctgtg actgcaacac ccgctgagaa
     5461 ttattgttta tctggttttc taaatgctgc tgttgcaact gagtttgcgt tttcagttgc
     5521 tgattcagca tccctttttg ctggatttgc tgagtctgca tctgggtttg catccgctgc
     5581 tggctgggta tctgataccc cggctggtta gggttgttca gagtattaat gggctgtgca
     5641 aagccgacaa acggcaggag tgccgtaaga atcagaagtc gtttcatcgc gtatcctcct
     5701 ctgaagatat cctttaagtt tactcgcttc ccgacaaaac gatgattaat tcagagttat
     5761 ataccaggct tagctggggt tgccccttaa tctctggaga ataacgatga taaaaccgac
     5821 gtttttacgc cgggtggcca ttgctgctct gctctcagga agttgtttta gcgccgccgc
     5881 cgcgcctcct gcgccgcccg tctcgtatgg tgtggaggaa gatgtcttcc acccggtacg
     5941 cgcgaaacag ggaatggtag cgtctgtgga cgccactgcc actcaggtgg gggtggatat
     6001 tctcaaggag ggcgggaatg ccgttgatgc cgccgtggcg gtgggctacg cgctggcggt
     6061 aacgcatccg caggcaggga atctgggcgg tggtggtttt atgttaatcc gctcgaaaaa
     6121 tggcaatacc acggctatca atttccgcga aatggcaccc gccaaagcga cccgcgatat
     6181 gttcctcgat gatcagggca acccggacag caaaaaatca ctcacttcgc atctggcttc
     6241 cggcacaccg ggtacggtag caggtttctc gctggcgctg gataaatacg gcaccatgcc
     6301 gctgaacaaa gtcgtgcagc ccgcgtttaa actggcacgc gatggtttta tcgttaacga
     6361 cgcgctggct gacgatctca aaacctacgg tagcgaagtg ttgccgaatc acgaaaacag
     6421 taaagctatc ttctggaaag agggcgagcc gctgaaaaag ggcgacacgc tggtgcaggc
     6481 gaacctggca aagagcctgg agatgattgc tgaaaacggc ccggacgaat tctataaagg
     6541 cacgattgcg gaacagatcg cccaggagat gcagaaaaac ggtggcttga tcactaaaga
     6601 agatttagcg gcctataaag cggtcgaacg cactccgata agcggcgatt atcgcgggta
     6661 tcaggtttac tccatgccac cgccatcctc cggcgggatc catatcgtac aaatcctcaa
     6721 tattctggaa aacttcgata tgaagaaata cggctttggc agcgccgatg cgatgcaaat
     6781 catggcagag gctgagaaat atgcctacgc cgaccgctcg gaatatcttg gcgacccgga
     6841 ttttgtcaaa gtcccgtggc aggcgctgac caataaagcc tatgccaaat ctattgccga
     6901 gcaaatcgat atcaataaag cgaaaccgtc cagcgagatt cgccctggca agcttgcgcc
     6961 ttatgagagt aatcaaacta cccattactc agtggtggat aaagatggta acgcggtggc
     7021 ggtgacctat acgctgaaca ccaccttcgg tacgggtatt gtcgcgggcg agagcggtat
     7081 tctgcttaat aaccagatgg atgatttctc cgccaaaccg ggcgtaccga acgtttacgg
     7141 gctggtgggc ggtgatgcca acgcggtcgg gccgaataaa cgcccgctgt cgtcgatgtc
     7201 accaaccatt gtggtgaaag acggtaaaac ctggctggtt accggtagcc ctggcgggag
     7261 tcggattatt accaccgtgc tgcaaatggt ggtgaatagc atcgattatg gcatgaatgt
     7321 cgctgaagcg accaatgcac cgcgcttcca ccatcaatgg ttgccggacg agctgcgtgt
     7381 cgaaaaaggg tttagcccgg atacgctcaa gctgctggaa gcaaaaggtc agaaagtggc
     7441 gctgaaagag gcgatgggca gtacacaaag cattatggtt gggccggacg gtgagttgta
     7501 cggcgcatcc gacccgcgct cggtggatga tttaacggcg gggtactaag gttagcggcc
     7561 ctcttcgtgg gaagagggct attttgtcag ggcaagccga aggtagcctt ttttatttcg
     7621 taatcctgta gatattcttc cagcggcttt gccgttccca gagaatgaat ttcaccactg
     7681 tctttatcaa taataaaagg tgcgttacca gctaagcgcg cggcctcatc tccagtttcg
     7741 agaaattctc gtgcttcgaa acagaaatac cagccctggc taaagcgtcc atgtagagta
     7801 atgacgaccg ggagatctgc atcatcaagg taatggttcg ctttcgcgaa tgcgtcgtga
     7861 taagtaatca taatta
//
LOCUS       DAZFYC010000179         7863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFYC010000179 DAZFYC010000000
VERSION     DAZFYC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.180"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..596
                     /locus_tag="ACHKW5_004754"
     CDS             72..596
                     /locus_tag="ACHKW5_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO7513970.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            680..1366
                     /gene="lpfB"
                     /locus_tag="ACHKW5_004755"
     CDS             680..1366
                     /gene="lpfB"
                     /locus_tag="ACHKW5_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO7513971.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNSNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     TGEMLTKPIN"
     gene            1390..3921
                     /gene="lpfC"
                     /locus_tag="ACHKW5_004756"
     CDS             1390..3921
                     /gene="lpfC"
                     /locus_tag="ACHKW5_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO7513972.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3932..4987
                     /gene="lpfD"
                     /locus_tag="ACHKW5_004757"
     CDS             3932..4987
                     /gene="lpfD"
                     /locus_tag="ACHKW5_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO7513973.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4993..5496
                     /gene="lpfE"
                     /locus_tag="ACHKW5_004758"
     CDS             4993..5496
                     /gene="lpfE"
                     /locus_tag="ACHKW5_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO7513974.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATV
                     DFTVQYE"
     gene            5749..7440
                     /gene="eptB"
                     /locus_tag="ACHKW5_004759"
     CDS             5749..7440
                     /gene="eptB"
                     /locus_tag="ACHKW5_004759"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO7513975.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7532..7608
                     /locus_tag="ACHKW5_004760"
     tRNA            7532..7608
                     /locus_tag="ACHKW5_004760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7566..7568,aa:Pro,seq:cgg)
     gene            7643..7776
                     /locus_tag="ACHKW5_004761"
     ncRNA           7643..7776
                     /ncRNA_class="other"
                     /locus_tag="ACHKW5_004761"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2050 a         1810 c         1987 g         2016 t
ORIGIN      
        1 ccatttgtat aatctgcgcc ccgttgtatg aacaacatca agcatattta tctatataaa
       61 tggagttata aatgaaaaag gttctgtttg ccttatctgc gcttgcgctg acctctactt
      121 ccgtattcgc agctgatgcg ggcgacggta ctgttaaatt caccggtgag attgttgacg
      181 ccgcttgtgt tgtgtctcca gacactcaaa aacaagaagt tgttctgggt caggtgaaca
      241 agtctgtatt tactgctatg ggtgataaat ccacagcaac gccgttcaaa atcaaactgg
      301 aaaactgcga tatctccact ttcaaaaatg tggaaatcag ctttaacggc gttggcgatg
      361 cagacaacag caaactggtt tctgtaagca ctgaaccagg tgccgcaact ggcgtaggta
      421 tcggtattta tgataacacc aataccctgg ttgacctgaa tactggtaaa tctgctactg
      481 tcctgaaaga aggtcagacc gttctgtatt tcaccgctaa ctatgtctct accaaagatg
      541 cagtaaccac gggttacggt aacgctgaag tcgacttcaa cctgacttac aactaatcga
      601 tcctgacgga acgatcatct ctcaatggca acagagactg tgttgccatt attcacacca
      661 atgactcagg gaccgaatca tgaaacgtat tagctttgct gtgctggcac ttttaatgac
      721 cgcacaacag tgttttgctg gtggcgtagc cttaagcacg acgcgtgtta tttatgatgg
      781 cggcaagaaa gaagcgtcgc tgaccgttga aaaccataat aaaaatgaag agtttttgat
      841 ccagtcatgg attgatgatg tgaatagcaa taaaaaaaca ccgtttatta ttacaccgcc
      901 attattcaaa cttgatcctg ataaaaataa cgtcttacgt atcgtcaata ttgaagatac
      961 cttacctaaa gatcgtgaat ccgtttactg gattaatgta aaggccattc ctgcgaaaag
     1021 tgctgaaaat gaaaataaaa acgtcttaca gatagccgtt cgcacccgtt tgaagttgtt
     1081 ctatcgtcca aaaggtttgc cgggaagcaa cctcgaggcc atgaaacagt tacgtttcca
     1141 gcgtcaaaat aaccaaattg tggttaataa cgactccgct tataacctga cctttaatca
     1201 gttttccatt aatggacaaa agattgaacg ggcgggaatg gttctcgcga aaggaaagtt
     1261 aaacattaac cttccagccg gtacaggcaa cgcgcacgaa gtgaaataca gcattattaa
     1321 tgatttcggt gttactggcg aaatgctgac gaagccgatt aactgatcct tttacaaggc
     1381 gcttttacta tgcattggat gcatttacct ctctatcact acagagcgca tttttccttc
     1441 tctctgctgg cattaacgat tgccagctcg ttacctgcgt atggcggaaa atttaaccct
     1501 aaatttctcg aaaacgttca gggtatcgat caacacatcg acttgtcggt atacgactca
     1561 cctgtcggcc agcaaatccc cggtaaatac cgggtttcgg tgtttgtaaa cgaagaaaaa
     1621 atggcttccc ggacgctgga ttttagtacc gccagcgaag cgaaacgcaa agccagcggt
     1681 gagtcgctta tgccttgcct tagccgggtg caactggaag aaatgggcgt gcgcgttgac
     1741 agctttccgg cgctgaagat gtcaccacca gaagcctgcg tagcgtttga tgaaatcatt
     1801 ccccaggcca ccagccgctt tgattttaat actcagacgc tgcatctttc ctttccacag
     1861 gcggcaatga tgatgaccgc tcgcggcacg gttgatcctt ctcgctggga cgaaggaata
     1921 cctgcgctgt tgctggatta cagctttagt ggcagcaatg gacgcaatga aggtacaggt
     1981 tcatcttcag attcgaccag tgacagttat tacctgaacc tgcgcagcgg tttgaacgtt
     2041 ggtccgtggc gtttacgtaa taacagtatc tggaaccgta ccgatggcaa aaaccaatgg
     2101 gataacgttg gtacatcgtt aaaccgggca attattccac ttaaatcaca gataactctg
     2161 ggtgataccg ccacgcctgg cgagattttt gatagcgtac aaatgcgtgg ggcgttactg
     2221 gcatccgacg atgaaatgtt gccggacagc cagcgtggct ttgctccggt agtgcgcggc
     2281 atcgccaaaa gcaacgctga agtgagtatc gaacagaacg gctatgtcat ttatcgcact
     2341 tttgttcagc cgggtgcatt tgagattaac gatctctacg caacctccgg cagcggcgac
     2401 ctgacggtga tcattaaaga agccgacgga agtgaacaac gttttatcca gccgttttcg
     2461 gcggtggcga ttttccagcg tgaagggtat ctgaaataca gtctggctgc cggtgaatat
     2521 cgtgctggta attacgacag cgataaacca catttcgggc agtttaccgc catgtacggc
     2581 ttgccgtggg gtatgaccgc ctacggtggt gcattgttgt cggcagatta caacgcttta
     2641 gctctgggcc tggggaaaaa cttcggtacc attggcgcag tatcggtgga tgtcactcag
     2701 gcgaaaagtc agttacgcaa caatgaaaaa gaggaaggcc agtcttatcg cttcctctat
     2761 tcgaaaagtt ttgagggtgg cactgacctg cgtctgttgg gctacaaata ctcaaccagc
     2821 ggctactaca ccttccagga agcaactgat gttcgcagtg acgccgacag tgattaccgc
     2881 cgttatcaca agcgcagcca gatacagggc aatattaccc agcaactggg agactacggc
     2941 tcggtttatt tcaatatgac gcagcaggat tactggaacg ttgacggtaa ggaaaactcc
     3001 ttaagcgccg gttatcacgg tcacattggt cgggtgaatt acagcgtggc ctatacctgg
     3061 acgagaagtc cggaatggga agaagacgat cgcttgtggt cattcagtgt ttccatccct
     3121 ctggggggcg cgtggagcag ttatcgcatg actaccgacc agaatggcaa gacgtcgcaa
     3181 caggcctctg ttagcggcac gttactggaa gatcgtaacc tgagctataa cgttcagcag
     3241 ggttacacca gtaatggcgt aggctacagt ggcagcgtca acatggggta tatgggtggc
     3301 tctggtaaca ttgacgtagg ctataactat tctaaagata accaacaggt taactatggc
     3361 gtgcgcggcg gtgtcatcgt tcacagtgaa ggtataacgc tttcgcagcc tttaggcgaa
     3421 agtctggcga ttgtttctgc accgggcgcg cgtggtggac atgtggttaa cagcagcggc
     3481 gtagaagttg actggatggg caacgccgtg gtgccttatc tcacaccata tcgtgaaacc
     3541 attgtggaat tgcgtagcga cacgttgggg caaaacgttg aactacagga agcgttccag
     3601 aaagtcgtgc ctactcgtgg tgcagttgtg cgttcccgct ttgatacccg cgtcggttac
     3661 cgtgtgctga tgagcttaaa acaggcgaat ggcaacgcag taccgtttgg tgcaaccgca
     3721 gcgttaattg atgagagcaa acctgccagc agcattgtgg gtgaagaagg acagctttat
     3781 atcagcggga tgccagaaga gggcgaattg caggtgagct gggggaacga gcaggcgcaa
     3841 cgttgccgtg tgccattccg cttgccagag aacaaggaca atgcggctat cgtgatggtt
     3901 aatgcggtat gcgagaaata aggatattta gatgaaaatt aaaaatttgt tggcaggtat
     3961 ggtgctgtta gggacgagca cttttgctgg aaatatatgg gctgcggatt ggggaccatg
     4021 tcagacatca aatggggtgg cccatgagta tagttttgat ttcgtacaga ctatccaggt
     4081 accttcagaa aataaggctg ggaaaatatt aactcagccc tttgctttag gtacaaaata
     4141 ttccgcttat tgtgaatgcc cggatcccat tccagataat ggagttgtga cttattttaa
     4201 aggtgtaacg cttctgcctg agcctggaac tgtagatggg tactataaat tcaataatta
     4261 cattgatatt ctcaccaaaa tatatgtcta cacacaatta gatatcccgg tgccttttac
     4321 cgatcgaagc aatggcacgg cacagaaaga atgtacgccc tatactgcta acaattgggg
     4381 gacaggtggt aaaggaagta tcagtattta tatttcacat ccttttgttg ggcaaatgat
     4441 tattcctaaa acacgagttg cgtccctctt tgggacaaaa aagaaaggtg tttacaatga
     4501 tagtcagccc atggccaatg tcagtatttc cgggagtatt acagttactc aaggctgtga
     4561 acttgccgct ggcaccgtac tggatattcc attcggagaa tatcaggccc atgattttaa
     4621 agggcgtgcc ggacaaccac cacaaaatgt tcagaaggta caaaaagaat taagctttga
     4681 ctgcaataat atttcagatg gcgtcaaaat ttatctcagt attgatgcaa caccaaatac
     4741 tgcctatccc agcgcaattg atttaggcaa cgccgatgtt ggtgcagtca ttgaggacgg
     4801 taaaggtaat attcttaagc cgaatgacag taatagctta ttagaaatga acccaggctc
     4861 gctgtatgaa gatgtcaaac gcaaagcgac aaccacaatt accgcttatc ccgttagcac
     4921 taccggaaaa ttacccgctg ccggggatta tagcggtatt gctaccatac atgtcgaact
     4981 ggagtaacag tcatgaagcg aacaatactt gcgttatgcc tgctgcctct ttccttttcg
     5041 tcattcgcgg caaccacgga tttagggccg aaaggaacat tgaaattcac ccttaaaatc
     5101 agccagggtg cttgcgaact ggtgaaagac agcgttgagg ttgacatggg caccgcggtt
     5161 ctcaaaaagc cggtaagaat cggaacggaa attaatccca ctcctttttc catcggcctg
     5221 aaaaactgta gcgaagtggt cagagcctac gtgactatgg acggtacacc caatgcagac
     5281 gactcaaatc tttttgcgct ggatgcgggt ggcgctacag gcgttgggct gaaaatcaaa
     5341 accgctgctg gagtacaaca gttcccggaa aatacaacgc cgacaccggt tgagtttgca
     5401 attaacggtg atggcgcaca tcaactgaac tatatcgcca gctacgtgcc tattcgcgca
     5461 gatgcaacgg tcgactttac tgtacagtac gaataatttc acatcttaag ggccagttaa
     5521 ctggcccttt ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta
     5581 aatttgtgct tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat
     5641 tcgcgcgtgt agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa
     5701 aagccgctaa gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc
     5761 aaatcgatta cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg
     5821 aatggcgcgg ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa
     5881 ggcgtttctg ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt
     5941 cttctatcgc tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt
     6001 tccgcaggtg ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc
     6061 gcttccgtca tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt
     6121 ctctggttaa tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc
     6181 tacaccttgc tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc
     6241 gtactggcgg gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa
     6301 gtggagaggg cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat
     6361 ctgccatcaa actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct
     6421 tccgataata attcattgct taatccggcg aagaaattca cttatcaggc accgcaaaac
     6481 gttgatgaca cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggc
     6541 attttcggct atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg
     6601 ttccgtggtt actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt
     6661 caggggggcg cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt
     6721 ctgaagcagt taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac
     6781 agcaacacga tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat
     6841 cgtggcaagc cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc
     6901 aacccggatg gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc
     6961 cagcgttatc cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc
     7021 tgtaccaaag cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt
     7081 atctccagcg tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac
     7141 cacggtgagt caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg
     7201 ccggagcagt tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg
     7261 gccaatgctc aggcatttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt
     7321 catgtagagc tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga
     7381 attaacgaaa acaacaactg gtgtcacatc ccgcagacaa aagtggcagc ggctaactaa
     7441 gcggcctgct gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg
     7501 cgtatctgcg cagtaagatg cgccccgcat tcggtgattg gcgcagcctg gtagcgcact
     7561 tcgttcggga cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc
     7621 ctgctcagcg agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc
     7681 ggggaggttc gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga
     7741 gccacgaagt ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag
     7801 caggcttttt tgcatttgta gctcatgagg atgagaacct ccgggggcgg ggaggttcga
     7861 ctc
//
LOCUS       DAZFYC010000180         7771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFYC010000180 DAZFYC010000000
VERSION     DAZFYC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.181"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(94..917)
                     /locus_tag="ACHKW5_004762"
                     /pseudo
     CDS             complement(94..917)
                     /locus_tag="ACHKW5_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(971..2689)
                     /gene="proS"
                     /locus_tag="ACHKW5_004763"
     CDS             complement(971..2689)
                     /gene="proS"
                     /locus_tag="ACHKW5_004763"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO7513976.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2801..3508)
                     /gene="tsaA"
                     /locus_tag="ACHKW5_004764"
     CDS             complement(2801..3508)
                     /gene="tsaA"
                     /locus_tag="ACHKW5_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO7513977.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3505..3909)
                     /gene="rcsF"
                     /locus_tag="ACHKW5_004765"
     CDS             complement(3505..3909)
                     /gene="rcsF"
                     /locus_tag="ACHKW5_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO7513978.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4027..4842)
                     /gene="metQ"
                     /locus_tag="ACHKW5_004766"
     CDS             complement(4027..4842)
                     /gene="metQ"
                     /locus_tag="ACHKW5_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO7513979.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4882..5535)
                     /locus_tag="ACHKW5_004767"
     CDS             complement(4882..5535)
                     /locus_tag="ACHKW5_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO7513980.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5528..6559)
                     /gene="metN"
                     /locus_tag="ACHKW5_004768"
     CDS             complement(5528..6559)
                     /gene="metN"
                     /locus_tag="ACHKW5_004768"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO7513981.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6747..7319
                     /gene="gmhB"
                     /locus_tag="ACHKW5_004769"
     CDS             6747..7319
                     /gene="gmhB"
                     /locus_tag="ACHKW5_004769"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO7513982.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            7683..>7771
                     /locus_tag="ACHKW5_004770"
     rRNA            7683..>7771
                     /locus_tag="ACHKW5_004770"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1941 a         1993 c         1959 g         1878 t
ORIGIN      
        1 gcatataatg caacattttg aattagcacg attttgtagg ccggataagg cgtttacgcc
       61 gcatccggca ttttgtgcac gaggctggct ttatcactga gtcagcccaa caaagcgacc
      121 cgccttgata taaatcccag gcttcaggtg cccaacatac tgtaactgcg tttctggctt
      181 aaacgcagag acatcaccaa tacgcatatg catcagatcg gcagggctaa tccagcccga
      241 ttgcgccagt gtaagtggat gcccggcttt ggcaaaggca tccgtgacaa attccgaaca
      301 aaaccacgac tttttgtctc cttcgcccac actgcttaac tgcgctttcg ccaggccgct
      361 gacgcacttt ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc
      421 ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg
      481 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
      541 agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc
      601 gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg
      661 aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt
      721 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
      781 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg gtctggctgg
      841 ctgatatcaa cggtacaagc cgaaagtaaa agaaagctgg ggagaaacag gcggcagtac
      901 gcctttggtt tatccatttt atacaatcca tgtaaaaaaa gggccctgaa attcaggacc
      961 ctttctggca tcagccttta atctgtttca ccagatattc gacgatgtca ccagtcttaa
     1021 ttaactgttt ctcgccgttg cgacgatatt tatattcgat atcgtcgttg tcgaggttac
     1081 ggtcgcccag cacaatagtg tgcggaatac cgatcagttc catatcagca aacatcacgc
     1141 ccggacgctc tttgcggtca tccagcagca cttcgatacc ttgtgcacgc agttcgctgt
     1201 acagtttctc agcaagctct tgtacgcgga aggatttgtg catgttcatc ggcagaatcg
     1261 ccacctggaa cggcgcgata gcgtcaggcc atacgatgcc tcgttcgtcg tagttctgct
     1321 caatcgccgc agctaccaca cgcgttaccc cgataccgta gcaacccatc gtcaggattt
     1381 ggttacggcc atcttcaccc tgtacggagg ctttcagtgc ttcggagtac ttggtaccca
     1441 gctggaagat gtgaccaact tcgataccac gtttgatcag cagcgtaccc tggccatccg
     1501 ggcttggatc gccagccacc acgttacgga tatctgcaac ttccggggta gcgacatcgc
     1561 gatcccagtt gatgccgaag tagtgtttac catcgatgtt agcaccagca gcgaaatcac
     1621 tcatcgccgc aacggtacgg tcaatcacca ccggaatcgg catgtttacc ggacccagtg
     1681 aacccggacc ggctttaacc acggcacgaa tttcttcttc ggtcgcgaaa gtcagcgggc
     1741 ttgcaacctg cggcagtttt tctgctttaa cttcgttcag ctcgtgatca ccgcgcacca
     1801 gcagcgcaac cagcgggaag ctactgcctt caaccgcttt aaccagcaga gtcttaaccg
     1861 ttttctcaat cggcagattg aactgttcaa ccagttccgc gatggttttc gcgttcggcg
     1921 tatcaaccag cgtcatttcc tgggtagcag cagcgcgcgg ttctttcggc gcgatagctt
     1981 ctgccagttc aatgttcgct gcatagtcag aggtgtcgga gaagaccaca tcgtcttcac
     2041 cgctctgcgc cagcacctgg aattcgtgag aggcgctgcc gccgatagaa ccggtgtcgg
     2101 cttgtacggc gcggaaatcc agccccatgc ggctgaagat tttgctgtag gccgcataca
     2161 ttgcatcgta ggtttcctgc agggattcct gagaagtatg gaaagagtaa gcatctttca
     2221 tcaggaattc gcgggaacgc atgacgccga aacgcggacg cacttcgtcg cggaacttgg
     2281 tctggatctg atagaagttc agcggcagct gtttgtaaga gctaagctcg ttacgaatca
     2341 ggtcagtgat aacttcttca tgagttgggc cgagtacgaa cggacgctcg ccacggtcaa
     2401 caaaacgcag cagttccgga ccgtactgtt cccaacgacc actctcttgc cacaaatcag
     2461 ctggctgaac caccggcatc gacacctcga tcgcaccggc gttgttcatc tcttcacgca
     2521 cgatgttttc gacttttttc agaacgcgca cgccggtcgg cagccaggta tataacccgg
     2581 aggccagctt gcggatcatc ccggcgcgca gcatcagctg atggctgatc acctcggcgt
     2641 cggcaggtgt ctccttgaga gtggagagca ggtattggct agtacgcatg ttgttacggt
     2701 tccagttgga aggtagaaca ggctcaaggc gagcctggga caaaaaaaag tgatttagtt
     2761 taccagtgca aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt tccagcgcaa
     2821 agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgt agccagacgg
     2881 cataggtttt gcccgtttcc tcacctttac gataggccgg gcgtgggtcc tgcgccagta
     2941 cttcgcggat aaacagcgtt aactgcggat aacgcttctc cagcgtcaaa agctgctttt
     3001 cgacctctgc ggtaaaactc accgccatct ctgccgctgg cgcactttgc gcatagctgg
     3061 cactggcatc gggaagcgat tcggcaaagg ggagatacgg tttgatatcc actaccggcg
     3121 taccatcgac cagatccaga ctgccgagct tcagaatcac gctgtcttta tggcaaacaa
     3181 cctctttcag ctctaccagc gacataccaa ttgggttagg gcggaaagta gagcgtgttg
     3241 cgaaaacccc cattctggcg ttaccgccga ggcgcggtgg acgcacagtc ggacgccagc
     3301 cgccttccat cgtttgatga aagacgaaaa ggatccataa atggctgaac gcttccaggc
     3361 cgcgaacggc gtcggcctgg ttgtagggag caatgagatg cagttcgccg ttggcgcttt
     3421 ttaccagacc tggctggcgc ggaacggcga atttttcttt atagggcgag cgaataacgc
     3481 ctatttgctc gaactggaaa ctgctcattt cgccgtaatg ttaagcgcag agccgataca
     3541 tacagcctga cgatagcagc ctggcgtacc gctggtgact tcgcagctat gcagtaatac
     3601 cgcattggcc ttcattttcg aggcgttgat ttgcatccgc ttacgcgcgg ttggaatgct
     3661 cggcggagag tcctgattag aggcctggca agagtcgcca ctgacttcac cgagatcgcg
     3721 gaacggtttg ccgactaatt cttctgcatt ggtataaatt cggaccggcg tggcgcgcgg
     3781 cgctttcggt tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa cgggttcgac
     3841 aggggatctg cttaacatgg aacagccgct tagcatgagt gctactaaac agatcggtaa
     3901 agcacgcata gtatttcctc aatgtatgat caaaacgtca atattgaatc aggagcttgt
     3961 aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt ggaaacagcc
     4021 taaaaattac cagcctttaa cagcgccgcc gttaaacact ttgtttgctg cttcgtaaac
     4081 ttcatcagac tgataagcct ggacgaattt cttcacgttc tcggcgtctt tgttatcttc
     4141 acgcgttacg atcaggttta cgtacgggga gtctttatct tcgacaaaga taccgtcttt
     4201 agctggagtc aggccaatct ggctggcata ggtggtattg ataactgcca gagcgatttg
     4261 cgcgtcgtcc agagatcgag gcagctgcgg tgcttccagc tcaacaattt tcagattttt
     4321 tgggttctca acaacatcaa gaacggtcgg cagcaggcca acgccatctt tcagtttgat
     4381 caagcccact ttttgcagca gcagcagtga acgaccaaga ttagttggat cgtttggcac
     4441 ggcaacctgc gaaccatcct gcagttcatc cagtgatttg attttcttgg agtaaccagc
     4501 aatcggataa acaaaagtgt tgcctactgc gaccagtttg tagccacgat ctttcagttg
     4561 ctgatcaagg tacggtttat gctggaaggc gttggcgtcg atatcgcctt tgctcaatgc
     4621 ttcgtttggc agaacatagt cgttgaaggt taccagctca acgtccaggc catatttgtc
     4681 tttcgcaact ttctgcgcga cttctgctac ctgctgttcg gcaccaacaa tcacgccgac
     4741 tttaatgtgg tttggatctt tttcatcctg accgcagcct accagtgcca gtgatccaat
     4801 cagggctccc actgccgcaa aggttttgaa tttgaacgcc ataccttatt ccttcttcaa
     4861 ttatttatgt tgtgttgaac gttacttgcg agtgacagcc cggacgatgc ggtcgcctgc
     4921 gaactgaatt aaataaacca gaatgaccag caataccagt accgtattca tcaccgtcgc
     4981 gttatagccg atgtagccat actgatagcc aatctgacct aaaccaccgg caccgactgc
     5041 accacccatc gcggaataac cgaccagggt aatcagggtg atagttgccg cattcaccag
     5101 acccggcaat gcttccggta acagcacttt acggacgatc tgcatcggcg tggcacccat
     5161 tgcgcgggaa gcttcaatta acccggttgg gatctccagc agagcgttct cgaccatacg
     5221 ggcaataaac ggtgctgcac caacggttaa cggaacaatc gctgcctgca aaccaatcga
     5281 tgtaccaaca ataacgcggg taaacggaat catccatacc agcaagataa tgaacgggat
     5341 agaacggaaa atgttcacaa tcgcagaaac ggtacgatac agcttcgcgt tagcaataat
     5401 ttgccccgga cgcgtgacat aaagcagaac gccaaccggc agaccaatca caaagccaaa
     5461 aaaaccggat acgaaggtca ttgccagcgt ttcccatacg ccacgaacca gcagccacat
     5521 catcggctca gacataaccc agtacctcta cttttacatg gtgttcctgc agccaggcaa
     5581 tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg ccgaacttaa
     5641 cgccaccggc gtagtccatc tgcgcgctaa taatgttgtt gttgacgttg aaacgacgcg
     5701 cggtttcaga aagcagtggg gcatcgaccg actgaccggt aaactccaga cgcagcatcg
     5761 gcacgcagtc agtgaatggc tccgcttgca gacgttcctg gtaatcttcc gggatatcca
     5821 gatgcagggt cgactgaata aacttctgcg ccaacggcgt tttcggatgc gaaaacactt
     5881 cacttaccgt gtcctgctcg atcagttcgc cattgctgat gaccgccacg caatcacaaa
     5941 tacgctttac aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc agacggcggt
     6001 taatgtcttt cagcagctcg agaatagaac gtgtcgttgc cgggtccagc gcgctggtgg
     6061 cttcatcaca cagcaatact ttgggattgc tggctaacgc acgggcaatc gccacacgct
     6121 gtttctgccc accggaaaga ttcgatgggt agctatcatg cttatcgcca agaccaacca
     6181 atgacagcaa ttccgtcacg cgacgtttga tctcgtcttt cggtgtgttg tccagctcca
     6241 gcggcagagc cacgttgcca aaaacagtac gcgaagagag caggttaaaa tgctggaaaa
     6301 tcataccaat ctggcggcga gctttggtca actcggattc tgacagcgtg gtcagttcct
     6361 ggccatcgac cagcacgcta ccctcggttg ggcgctccag caggtttaca caacgtataa
     6421 gcgtactctt acccgcgcct gaggcaccga taacgccata aatttgtcca gctggcacat
     6481 gcaggctgac gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac actttggtga
     6541 tattcgaaag ttttatcatt gattatttat tatcgtcatt aagttagtcg tggcatctcg
     6601 aatgcctgaa acgggcaacg ccgtcaatga aatggatgtt aaggcatcca gacgtctaaa
     6661 tcaatcaggt ttatgcgaag agcactttct tgcaggtcga aacatgcgat actagcgtca
     6721 catgccttat taaggagcta taaaaggtgg caaagagcgt acccgcaatt tttcttgacc
     6781 gtgatggcac cattaatgtc gatcacggct atgtccatga gatcgacaac tttgaattta
     6841 tcgacggtgt tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg ctggtggtag
     6901 taaccaacca gtctggcatt gctcgcggta aatttaccga agcacagttt gaaacgctga
     6961 ccgagtggat ggactggtcg ctggcggacc gagatgtcga tctggatggt atctattatt
     7021 gcccgcatca tccgcagggt agtgttgaag agtttcgcca ggtctgcgac tgccgtaaac
     7081 cacatccggg gatgcttttg tcagcacgcg attatttgca tattgatatg gccgcttctt
     7141 atatggtggg cgataaatta gaagatatgc aggcagcggc tgcggcgaac gtgggaacaa
     7201 aagtgctggt gcgtacgggt aaacctatta cgcctgaagc agaaaacgcg gcggattggg
     7261 tgttaaatag cctggcagac ctgccgcaag cgataaaaaa gcagcaaaaa ccggcataat
     7321 gattaaaaga tgagcaattg aaataaaaat gcatttttcc acttgtcttc ctgagacgac
     7381 tccctataat gcgcctccat cgacacggcg gatgtgaatc acttcacaca aacaaccggt
     7441 tcggttgaag agaaaaatcc tgaaattcag ggttgactct gaaagaggaa agcgtaatat
     7501 acgccacctc gcgacagtgc gctaaagcgc gtcgcaactg ctctttaaca atttatcaga
     7561 caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac
     7621 caagtctcaa gagtgaacac gtaattcatt acgaagttta attctttgag catcaagctt
     7681 ttaaattgaa gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc
     7741 aagtcgaacg gtaacaggaa gaagcttgct t
//
LOCUS       DAZFYC010000181         7713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFYC010000181 DAZFYC010000000
VERSION     DAZFYC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.182"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(153..1334)
                     /locus_tag="ACHKW5_004771"
     CDS             complement(153..1334)
                     /locus_tag="ACHKW5_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFO7513983.1"
                     /translation="MCMNPFPQQNKSYQFYYDESNNVRKLYLSKQIDGYNIDHDPDKH
                     NSVNFVLAGVAHTGSSSSADFDDLRQRIQLQANAKEFKLKHLAKGDFLTMLTSKKLTA
                     FFEWLLYGDLYLHYFHLNMEYWGYVDIIDDCILFGREKGFIPEMSDEQFYGYMLANKD
                     ALHTYVKANKVPFIQFLKSYDFPYIEGREQAFIQGLLSQISAHCVNLYTATNRDEFQL
                     RLAHGLLQLLMACSDQNIDDMTLTMDIRDEPPTDDDNRLVDGFAIFYQNRAQMFEASS
                     HIFDIEKVVEADLQKAAEANPNLTLNYSFADSKLNPLVQVSDVVAGFMQHYFDYLNSH
                     SYEQIKHDRNSLNERQRLNMEFLRLLINKSHDNNPTLLHCVMSALEHEKHKTFTYPDE
                     P"
     gene            1521..1652
                     /locus_tag="ACHKW5_004772"
     CDS             1521..1652
                     /locus_tag="ACHKW5_004772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7513984.1"
                     /translation="MSHNLDHSRMVKEVLKAVARGDGNAEVVVTYQGSDAQWNENSR"
     gene            complement(1763..2623)
                     /locus_tag="ACHKW5_004773"
     CDS             complement(1763..2623)
                     /locus_tag="ACHKW5_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO7513985.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(2806..3363)
                     /locus_tag="ACHKW5_004774"
     CDS             complement(2806..3363)
                     /locus_tag="ACHKW5_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7513986.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            3665..4429
                     /locus_tag="ACHKW5_004775"
     CDS             3665..4429
                     /locus_tag="ACHKW5_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFO7513987.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4922..5506)
                     /gene="mphR(A)"
                     /locus_tag="ACHKW5_004776"
     CDS             complement(4922..5506)
                     /gene="mphR(A)"
                     /locus_tag="ACHKW5_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFO7513988.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(5506..6744)
                     /gene="mrx(A)"
                     /locus_tag="ACHKW5_004777"
                     /gene_synonym="mrx"
     CDS             complement(5506..6744)
                     /gene="mrx(A)"
                     /locus_tag="ACHKW5_004777"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFO7513989.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(6741..7646)
                     /locus_tag="ACHKW5_004778"
     CDS             complement(6741..7646)
                     /locus_tag="ACHKW5_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFO7513990.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         1845 a         2087 c         2092 g         1689 t
ORIGIN      
        1 ataagtgata ttggttgtct ggagattcag ggggcctgtc taatcatata ttcaataaaa
       61 atcaaattaa attgagatat taaataataa aacaagataa gcggtatagg taaacacaaa
      121 ataaaagggg cattaagccc cttcatagtt atttatggtt catccgggta agtaaatgtt
      181 ttatgttttt catgctctaa agctgacata acacaatgta ataatgttgg gttattatca
      241 tgagatttgt ttatcaggag ccttaaaaac tccatattta atctttgcct ttcgttaagg
      301 ctgtttctgt catgtttaat ctgttcgtag ctatggctat tgagataatc aaaatagtgc
      361 tgcataaagc cagccacaac atcactcact tgaaccagag gatttagctt tgaatcagcg
      421 aaagagtaat taagcgttag attagggttg gcttctgctg ccttttgaag atctgcttcc
      481 actaccttct ctatgtcaaa gatatggctg gaagcctcga acatttgcgc ccggttctgg
      541 tagaaaatag caaaaccatc aacaaggcgg ttgtcgtcgt ctgtaggcgg ttcatcccta
      601 atatccatag tgagtgtcat atcatctatg ttttggtcag agcaggccat caggagctgc
      661 aggaggccat gagctaaccg aagctgaaac tcatctctat tggtagctgt atagaggttt
      721 acgcaatgag cggagatctg acttaatagc ccttgtatga aagcctgttc cctcccctcg
      781 atgtatggga agtcatagga cttgaggaac tggatgaagg gaaccttgtt agccttaacg
      841 taggtgtgta gagcatcctt gtttgccaac atgtagccat agaactgttc atcactcatt
      901 tccgggatga agcccttctc acgtccgaac aggatacaat catcaatgat gtccacgtat
      961 ccccaatact ccatattcag gtggaagtag tgtaggtata gatcaccata gagaagccac
     1021 tcaaagaacg ctgtgagctt cttagacgtg agcatagtca ggaagtcgcc tttggctaag
     1081 tgtttcagct taaactcttt ggcgttggcc tgtagctgta tgcgctggcg taaatcatca
     1141 aagtcggcac tggatgagga accagtatga gcaacaccgg ctaacacgaa gtttacgctg
     1201 ttgtgcttgt ctggatcatg gtcaatgtta taaccatcaa tttgtttgga tagatatagt
     1261 ttacgaacgt tattagattc atcgtaatag aattgatagc ttttgttttg ctgcgggaaa
     1321 ggattcatac acatcacttt tttgtgggtt atgaaccata tataacaatg taggatctgg
     1381 aagtctataa acaaaaaacc cgcacagggc gggcttcttt aagagagagg atttgattta
     1441 gaaccacggc agcctggcgg ctgcaaatcg gggtgtggca gaatatgagc tttcagagta
     1501 accatccaga atgagttccc atgtcacaca acctggacca cagcagaatg gtgaaagagg
     1561 ttctgaaagc cgtcgccaga ggggatggca atgccgaagt cgttgtcaca tatcaggggt
     1621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     1921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     1981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2041 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     2101 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2161 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2221 agttggcagc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2281 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2341 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcaccac
     2401 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2461 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2521 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2581 caaaaaaggg aataagggcg acacgaaaat gttgaatact catactcttc ctttttcaat
     2641 attattgaag catttaccag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     2761 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     2821 gtcttcaaga attttataaa ccgtggagcg ggcaatactg agctgatgag caatttccgt
     2881 tgcaccagtg cccttctgat gaagcgtcag cacgacgttc ctgtccacgg tacgcctgcg
     2941 gccaaatttg attcctttca gctttgcttc ctgtcggccc tcattcgtgc gttctaggat
     3001 cctccggcgt tcagcctgtg ccacagccga caggatggtg accaccattt gccccatatc
     3061 accgtcggta ctgatcccgt catcaatgaa ccggactgcc acgccctgag cgtcaaattc
     3121 ctttatcagt tggatcatat cggcagtgtc gcggccaaga cggtcgagct tcttaaccag
     3181 aatgacatca ccttcctcca ccttcatcct cagcaaatcc agcccttccc ggtctgttga
     3241 actgccggat gccttatcgg taaatatacg gtttgctttc acacctgcgt ctttgagtgc
     3301 tctgacctga agatcaagag actgctgact ggttgagacc cgagcgtaac caaaaagtcg
     3361 cataaaaatg taccttaaat cgaatatcgg acaactcatg tctattatta caaatttacg
     3421 atttaataga catattaatg taacagtttt acgatgtccg ataatttata acatttcgta
     3481 cggttggaaa aatgttacta aatgcccgtc aggcagggag gccgatatgc ccgttgactt
     3541 tctgaccact gagcagactg aaagctatgg cagattcacc ggtgaaccgg atgagcttca
     3601 gctggctctg ttgcaaaaat cgtgaagctt gagcatgctt ggcggagatt ggacggacgg
     3661 aacgatgacg gatttcaagt ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg
     3721 ctggtattgt cgctatccga tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg
     3781 catttcggtc gaccatacga cgatctatcg ctgggtccag tgctacgccc cggagatgga
     3841 gaagcggctg cgctggttct ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac
     3901 ctacgtcaag gtgcggggca agtggaccta cctgtaccgg gcagtcgaca agcggggcga
     3961 cacgatcgat ttctacctgt cgccgacccg cagcgccaag gcagcgaagc ggttcctggg
     4021 caaggccctg cgaggcctga agcactggga aaagcctgcc acgctcaata ccgacaaagc
     4081 gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac
     4141 ggcccaccgg caggtgaagt atctcaataa cgtgatcgag gccgatcacg gaaagctcaa
     4201 gatactgatc aagccggtgc gcggtttcaa atcgatcccc acggcctatg ccacgatcaa
     4261 gggattcgaa gtcatgcgag ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc
     4321 cggcatcagg ggcgaggtgc gccttgtgga gagagctttt ggcattgggc cctcggcgct
     4381 gacggaggcc atgggcatgc tcaaccacca tttcgcagca gccgcctgat cggcgcagag
     4441 cgacagccta cctctgactg ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct
     4501 cggtgatggt ggagtgaagc caacccgcaa tcgggttatg aatctgcatc gcgattcgca
     4561 atcagctgtc tcttgagcat gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc
     4621 tacaccaact tgtccaactc atccgccaca ccgctcccca ccacctgcga tggccaggtt
     4681 gtttgctggg cgcattggcg gagtatcgtc tcgacccggg acacccacac cggcacaacc
     4741 ttacgggagt gattcactgt caaagaatcg gcccggtgct ctgacgcaag tatcgggatg
     4801 gtcaccattt gtaagccgta gacctgagtg gtgatcaaga cttcgatacc accgaccgta
     4861 ccggtactaa tcgacgacgg tcgtgttcgt cgcctgccgc agggactctg cacacctccg
     4921 tttacgcatg tgcctggagg agttggaaat cgtcgtgttc gggaaacatt aaacacagga
     4981 tggcagcgat ctgagccagc acatgatcag ctagctcacc atccggatcg acggcccact
     5041 gcatcgtcgc gccagcgatg accgagtgca ggagcaactc agctgccgca ggagcacctg
     5101 ggggcagtcg cttgcggatc ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc
     5161 gtagctccgg cacctggagc tcgtaccagg agatgagata gttcaccgag aagtcgttgc
     5221 gagtgttcat gctccgaacg agcacctgca aaaattccca gagcccttgc ggccctgcgc
     5281 ctatcggtat cgcattcagg taatgccgca cctgctcgac gccgcgctcc atcatcctca
     5341 ccagcagcgt atcgcggttg gtgaagcgct ggattaacgc tgcgcgggag agccccacct
     5401 cctttgctac tccgctgagc gtgaactcta tgggaccgca acgcttcagc actacggtgg
     5461 cggcctcgag tacctcgtca tcggacttga gcttggggcg gggcatcagt gttcaccttc
     5521 tgtatgggtt ggggcggagg ctgtggctgc cgccgccatt gtagcaaatt gaagacggag
     5581 cgagagtaga gccacgagcc ccgcaaacac ggccgataca acgaggccag ggagcggacc
     5641 agcaaggtcg acaaacgcgc cggccgcaag cataaccatg ggcgaggctg acagcatcac
     5701 cgccgagacc gtgccgagta cccggccgag aagttctggc ggcgtgcggt tgtagatggc
     5761 agcgttgaga atgggagaga ctgagccggt cagcagtccc acgagcgcgc ccaacaacat
     5821 cagcaccggc acgcctggca actgtgaaag cagaagcgag cccaccgcag agccacaaaa
     5881 tgccaccgcc agccagttct gcgctgatat ccgggcgccg accgacgcat gaatggcaat
     5941 gccaaggaga ccaccagccc ccatcattga ggagaacagc ccgagctctg ctacttggcg
     6001 tcctgcatct acaaacagcg caggcatgat gacgctgccg ttggcgccaa cgatgcccac
     6061 gaagatcatc actataccaa agagagggcg cagcaggggt tcgctccaga gaaaagcgac
     6121 gccggcgcgc atggagagag tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag
     6181 cacccacgcg ccgagcagac ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg
     6241 cgcagcgcca agcaggccga ttgcggcccc cccaagggcc gggccaccta gaatcgcgac
     6301 gttcccgatc accgctttca gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc
     6361 gagtttgggc agctcactgt cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag
     6421 caccacgcaa gcgatcagcc cagtgttcga gagggcgcca acggcatcga gcagtgggat
     6481 gctcgccatg gccacgccgc ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa
     6541 ccgatcgacg aggccaccac caacccacgc gccgatgatg gtcgcgatga cgctgctagc
     6601 ggccgtggcg cccgcccagg ccgcgctctt tgtatgagac aggacgaacc atggaagcgc
     6661 gagggccgcc accgcgttgc cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag
     6721 cgggctatat cgacgttcgc tcattccgct gcggcgagct gcgccttcgc cgcagcgagg
     6781 tactcttcgt tacccgagtc gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg
     6841 cgctccgcga tgtggtgggc gagccgcggc cacacccggc caccggccgc ttcatacgtg
     6901 aggaggagct tcgcgagccc ctcttcacca aagaccataa ggtgcgcggc catgtcgatg
     6961 gcagggtcat caacgcgggc ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg
     7021 tcgatgagca catggcccac gtagagatcg ccatgcacca ccacggagaa atctggccac
     7081 gacgaatcgt cgtcgagcca gcgctgccac cggtggaggc gcttgtcgtt caccacgaac
     7141 tcgcgtcgga cgcggtcaac gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg
     7201 atgagcatcc ccgcatccac ggcggcggaa atggggacgg catgcagggc ggcgagcgcg
     7261 gtcgcgaagc tctccgcgaa gacctccgag tcctgcggca cgacccagtc gggcgtggac
     7321 gaaccaggct ggatgaccat cgcagtcgag tcttcgagca tgggataggc aacgagctcg
     7381 gcgttggcca cgcgccagtc cggcaccgcg aacggcaggc gattcttgag cattgccagc
     7441 acccgcgcct ctggttcgac cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc
     7501 caccgacgtc catcgtcgac ggtggcgatc acgatcctat agtcgagccc aagctcattg
     7561 acagtcagcg ggccatggag cttgagccca tgtcgggctg caagtgcgta cagttgggag
     7621 gtatcggcgg tcgtgactac ggtcatgatt cactcctgag ggcttgacgg gtttagccac
     7681 ctaaatgtaa cagtcacgtc ggttatattc aat
//
LOCUS       DAZFYC010000182         7689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFYC010000182 DAZFYC010000000
VERSION     DAZFYC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.183"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            31..552
                     /gene="fecI"
                     /locus_tag="ACHKW5_004779"
     CDS             31..552
                     /gene="fecI"
                     /locus_tag="ACHKW5_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO7513991.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            549..1502
                     /gene="fecR"
                     /locus_tag="ACHKW5_004780"
     CDS             549..1502
                     /gene="fecR"
                     /locus_tag="ACHKW5_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO7513992.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1589..3913
                     /gene="fecA"
                     /locus_tag="ACHKW5_004781"
     CDS             1589..3913
                     /gene="fecA"
                     /locus_tag="ACHKW5_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO7513993.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            3958..4860
                     /gene="fecB"
                     /locus_tag="ACHKW5_004782"
     CDS             3958..4860
                     /gene="fecB"
                     /locus_tag="ACHKW5_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO7513994.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4857..5855
                     /gene="fecC"
                     /locus_tag="ACHKW5_004783"
     CDS             4857..5855
                     /gene="fecC"
                     /locus_tag="ACHKW5_004783"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO7513995.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5852..6808
                     /gene="fecD"
                     /locus_tag="ACHKW5_004784"
     CDS             5852..6808
                     /gene="fecD"
                     /locus_tag="ACHKW5_004784"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO7513996.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6809..7576
                     /gene="fecE"
                     /locus_tag="ACHKW5_004785"
     CDS             6809..7576
                     /gene="fecE"
                     /locus_tag="ACHKW5_004785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO7513997.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRAVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1609 a         2248 c         2195 g         1637 t
ORIGIN      
        1 acgtgataat taacttttga tgcactccgc atgtctgacc gcgccactac cacagcttcc
       61 ttaacgttcg agtcgcttta tggcacacat cacggctggt tgaaaagctg gctgacgcgc
      121 aaactccagt ctgcttttga tgcagatgac attgcccagg acactttttt gcgggtaatg
      181 gtcagcgaaa cgctctcgac gatccgcgat cctcgatcct tcctctgcac tatcgccaaa
      241 cgcgtgatgg tggacctgtt tcgccgaaac gcgctggaaa aagcgtatct ggagatgctg
      301 gcgcttatgc cggagggggg agcgccttca cctgaggaac gcgaaagcca actcgagacc
      361 ctacaactcc tcgacagcat gctggacggg ctaaacggca aaacacgtga agcgtttctg
      421 ctttcgcaac tggatggtct gacatacagc gagattgcgc acaaactcgg tgtttccatc
      481 agctccgtga aaaaatacgt ggcgaaagcc gtcgagcact gcctgctgtt ccgtctggag
      541 tatgggttat gaatcctttg ttaaccgatt cccgccgtca ggcgctgcgt tcagcttccc
      601 actggtatgc cgtgctaagc ggcgagcgcg tcagcccaca acaggaagcg cgctggcaac
      661 agtggtatga acaggatcag gataaccagt gggcctggca gcaggttgaa aacctgcgca
      721 accagcttgg cggtgtgcct ggcgacgttg ccagccgggc gttgcacgat acccgcctca
      781 cccgccgtca cgtgatgaaa ggattactgc tgttgctcgg cgctggcgga ggctggcagc
      841 tctggcagtc ggaaaccggc gaaggtctgc gggcagatta ccgcaccgcc aaaggcacag
      901 tcagccgtca gcaactggaa gatggctccc tgctcacgct gaatacccaa agcgcggcgg
      961 atgtgcgttt tgatgcgcat cagcgcaccg tccggctctg gtacggtgaa atcgccatta
     1021 ctaccgcgaa agatgccctg caacgcccct tccgtgtcct gacccgtcag ggccagctca
     1081 ctgctttagg gacagaattt accgtccgcc agcaggataa tttcacgcag cttgacgttc
     1141 agcagcacgc tgtggaagtg cttctcgcca gtgcccccgc gcaaaaacgc atcgtgaacg
     1201 ctggtgaaag cctgcagttc agcgcctctg agtttggcgc agtgaaaccg ctggatgacg
     1261 agagtacaag ctggacgaag gacatcctga gcttcagcga taaaccgctg ggtgaggtga
     1321 tagccacgct aacccgttac cgcaacggcg tgctgcgctg cgatcccgcc gttgccgggc
     1381 tgcgcctgag cgggacgttc ccgctgaaaa ataccgatgc gatcctgaac gttatcgcgc
     1441 aaacgcttcc cgttaaaatt cagtctatta cgcggtactg gataaacatt tcaccactgt
     1501 aaggaaaata attcttattt cgattgtcct ttttaccctt ctcgttcgac tcatagctga
     1561 acacaacaaa aatgatgatg gggaaggtat gacgccgtta cgcgtttttc gtaaaacaac
     1621 acctttggtt aacgccattc gcctgagcct gctgccgctg gccggtctct cgttttccgc
     1681 ttttgctgca caggttaata tcgcaccggg atcgctcgat aaagcgctca atcagtatgc
     1741 cgcacacagc ggatttaccc tctcggttga cgccagcctg acgcgcggca agcagagcaa
     1801 cggcctgcac ggcgattacg acgtcgagag cggcctgcaa caactgctgg acggcagcgg
     1861 actgcaggta aaaccgctgg gaaataacag ctggacgctg gagcccgcgc ccgcaccaaa
     1921 agaagatgcc ctgaccgtgg tcggcgactg gctgggtgat gcgcgtgaaa acgacgtatt
     1981 tgaacatgct ggcgcgcgtg acgtgatccg ccgtgaggat ttcgccaaaa ccggcgcaac
     2041 caccatgcgt gaggtactta accgcatccc tggcgtcagc gcgccggaaa acaacggcac
     2101 cggcagccac gacctggcga tgaactttgg catccggggc ctgaacccgc gcctcgccag
     2161 ccgctcgacc gtcctgatgg acggcatccc cgtccccttc gccccttacg gtcagccgca
     2221 gctttcactg gctcccattt cgctcggcaa catggatgcc attgacgtgg tacgcggtgg
     2281 tggtgcggtg cgttacggac cgcagagcgt gggcggcgtg gtgaactttg ttacccgtgc
     2341 cattccgcag gactttggta tcgaggcggg cgtggaaggt cagctcagcc caacctcttc
     2401 acaaaacaac ccgaaagaga cgcacaacct gatggtgggc ggcacagcgg acaacggttt
     2461 tggcaccgcg ctgctctact ccggcacgcg cggcagtgac tggcgcgagc acagcgccac
     2521 ccgcatcgac gacctgatgc tgaaaagcaa atatgcgccg gatgaggtgc acaccttcaa
     2581 cagcctgctg caatattacg acggtgaagc cgacatgccc ggtggcctgt ctcgcgcgga
     2641 ttacgacgcc gatcgctggc aatccacccg cccgtatgac cgcttctggg gtcgtcgcaa
     2701 gctggcgagc ctgggctacc agttccagcc agacagccag cataaattca acattcaggg
     2761 gttctacacc caaaccctgc gcagcggcta cctggagcaa ggcaaacgca tcaccctctc
     2821 gccgcgtaac tactgggtgc gcggtattga gccacgctac agccagatct ttatgatcgg
     2881 cccttccgcg cacgaagtgg gcgtgggcta tcgctatgtg aatgaatcaa cgcatgaaat
     2941 gcgttactac accgccacca gcagcgggca gttgccgtcc ggctcaagcc cttacgaccg
     3001 cgatacgcgt tccggcaccg aggcgcacgc ctggtatctg gatgacaaaa tcgacatcgg
     3061 caactggacc atcacgccgg gtatgcgttt cgaacatatc gagtcatacc agaacaacgc
     3121 catcacaggc acgcacgaag aagtgagcta taacgcaccg cttccggcgt tgaacgtgct
     3181 ctatcacctg actgacagct ggaatcttta tgcaaacact gaaggctcgt tcggcaccgt
     3241 acagtacagc cagattggca aggctgtgca aagcggcaat gtggaaccgg aaaaagcgcg
     3301 aacctgggaa ctcggtaccc gctacgacga cggcgcgctg acggcggaaa tggggctgtt
     3361 cctgattaac tttaacaatc agtacgactc caaccagacc aacgacaccg tcactgcacg
     3421 tggcaaaacg cgccataccg ggctggaaac gcaggcacgt tacgatctgg gtacgctaac
     3481 gccaacgctt gataacgttt ccatctacgc cagctatgcg tatgtgaacg cggaaatccg
     3541 cgagaaaggc gacacctacg gcaatctggt accattctcc ccgaaacata aaggcacgct
     3601 gggcgtggac tacaagccag gaaactggac gttcaatctg aacagcgatt tccagtccag
     3661 ccagtttgcg gataacgcca atacggtgaa agagagcgcc gacggcagta ccggccgcat
     3721 tcccggcttc atgctctggg gcgcacgcgt ggcgtatgac tttggcccgc agatggcaga
     3781 tctgaacctg gcattcggtg tgaaaaacat cttcgaccag gactacttca tccgctctta
     3841 tgacgacaac aacaaaggca tctatgcagg ccagccgcgc acgctgtata tgcaggggtc
     3901 gttgaagttc tgatgtgtta acgcccggct tgccgggctt ttagctggaa tgtgattatg
     3961 ttggcattta tccgttttct ttttgcaggc ctgctgctgg tgatcagcca cgcctttgcc
     4021 gccatggttc aggacgaaca cggcacgttt acactcgaaa aaacgccaca acggattgtg
     4081 gtgctggaac tctcgttcgc cgatgcgctg gccgccgtgg acgtcagccc gatcggtatt
     4141 gccgacgata acgatgcaaa acgcatcctg cccgaagtgc gtgcgcacct gaaaccgtgg
     4201 cagtccgtcg gaacgcgcgc gcagccgagc ctggaagcca ttgccgctct gaaaccagac
     4261 ctgatcattg ccgacagcag tcgccatgcg ggggtttaca tcgccttgca gcaaatcgcg
     4321 ccggtactgc tgcttaagtc ccgcaacgaa acctacgctg aaaatttgca atctgcggct
     4381 atcatcggcg aaatggtggg taaaaagcga gagatgcagg cacgtctgga acaacataaa
     4441 gagaggatgg cgcagtgggc cagccagctt cccaaaggga cacgcgtggc ctttggcaca
     4501 tcacgcgaac agcaattcaa cctgcatact caggagacct ggaccggcag cgtgctggcc
     4561 tctctggggc tgaacgttcc cgctgcgatg gcgggttcgt ccatgccgtc catcggcctg
     4621 gagcaactgc tggcggtcaa tcctgcctgg ctgctggttg cccactatcg cgaagagagc
     4681 attgttaaac gctggcaaca agatccgctc tggcagatgt taaccgccgc gcagaagcag
     4741 caggttgctt cggtcgacag taacacctgg gcgcggatgc gcggtatttt tgctgcagag
     4801 cgtattgccg ctgacacggt aaaaatcttc caccatcagc cgcttaccgt tgtgaaatga
     4861 ccgcgataaa acacccgatg ctgctgtggg ggcttcccgt tgcagcactt attattattt
     4921 tctggctgag tctgttttgc tactcggcca ttcctgtttc cggagcagat gcaacccgcg
     4981 ccctgctgcc tggacacacg ccaacgctac cagaagcgct ggtgcaaaac cttcgtttgc
     5041 cacgaagcct ggtcgccgtt ctgatcggcg caagcctggc gctcgcgggc acgctgctgc
     5101 aaaccctgac ccacaaccca atggcctctc cttcactgct cggcattaac agcggcgcgg
     5161 cgctggctat ggcgcttacc agcgcgctga gtccgacgcc gattgcaggc tattctctgt
     5221 cgttcatcgc ggcatgcggg ggcggcgtga gctggctgct ggtcatgacc gcaggaggcg
     5281 gatttcgtca tacccatgac agaaacaaac tgatcctcgc gggtatcgcg ctgtcggcct
     5341 tttgtatggg ccttacccgc atcaccctgc tgctggccga agatcatgct tacggcatct
     5401 tttactggct ggcaggcgga gtgtcccacg cccgctggca ggatgtctgg cagctcttgc
     5461 cggtggtggt cactgcagtc cctgtcgtgt tactgctggc gaatcaactg aacctgctca
     5521 acctcagcga cagcaccgcc catacgctgg gagtgaacct gacgaggcta cgtttggtca
     5581 tcaatatgtt agtgctgctt ctggttggtg cgtgcgtcag cgtggcaggt ccggtggcgt
     5641 ttatcggtct gctggtgcca catctggcgc gcttctgggc aggcttcgat cagcgcaacg
     5701 tactgccagt gagcatgctg ctgggggcca cgctgatgct gctggcagat gtactcgcac
     5761 gcgcgctggc cttccccgga gatctgcccg caggcgcagt gctggcgctg attggcagcc
     5821 cttgctttgt ctggcttgtg aggaggcgag gatgaaaatt gcgctggtta ttttcatcac
     5881 ccttgccctg gcgggctgtg cgctgttatc actccatatg ggagtgatcc ccgtgccgtg
     5941 gcgcgcgctg ctgaccgact ggcaggccgg acgcgagcat tattatgtat tgatggagta
     6001 ccgactgccg cgcttgctgc tggcactgtt tgtcggtgca gccctcgccg tggcgggcgt
     6061 gctgatacag gggattgtgc gcaaccctct ggcatcaccg gatattctcg gcgttaacca
     6121 tgccgccagc ctggcctctg tgggggctct acttcttatg ccgtcactgc ccgtgatggt
     6181 gctgccgctg ctggcctttg cgggcggcat ggcggggttg atattactga agatgctggc
     6241 aaagacccac cagccgatga agctggcgct caccggcgtg gcgctttctg catgctgggc
     6301 cagcctgacg gattatctga tgctctcgcg cccgcaggat gtgaacaacg ccctgctgtg
     6361 gctgaccggc agcttatggg gccgtgactg gagctttgtg aagattgcca tcccgctgat
     6421 gattttattt ctgccgctga gcctgagttt ttgccgcgat ctcgacctcc ttgcactcgg
     6481 cgatgcgcgc gccaccacgc tcggtgtgtc ggtgccccat acccgattct gggctttgtt
     6541 actagctgtc gccatgacat ctaccggcgt ggccgcctgc ggcccgatta gctttattgg
     6601 tctagtggtg ccgcatatga tgcgtagcat caccggtgga cgtcaccgca gactgctgcc
     6661 tgtttcagcc ctgacaggtg cgttgctgtt ggtggttgcc gatctgctgg cgagaattat
     6721 tcatccccca ctggagctcc cggttggcgt gctgaccgcc attatcggtg cgccgtggtt
     6781 tgtctggttg cttgtgagaa tgcgataaat gactttacga actgaaaatc tgacggtcag
     6841 ttacgggaca gacaaggtac ttaacgacgt ttcactctca ctgccaacgg ggaagatcac
     6901 cgccctgatc ggccctaacg gttgcgggaa atcgacgctg ttaaactgtt tttcgcggct
     6961 tttaatgccg cagtctggca ccgtatttct cggcgataat cccataaata tgctctcatc
     7021 acgccagttg gcccgcaggc tttcgctgct gcctcagcac catttaacgc cagaggggat
     7081 cacagtccag gagctggttt cgtatggtcg taatccctgg ctgtcactct gggggcgtct
     7141 ctccgctgaa gacaatgcac gagttaatgt cgccatgaac cagacccgga tcaatcatct
     7201 tgccgttcgt cggttaaccg agctttccgg cggtcagcgc cagcgcgcat ttctggcgat
     7261 ggtcctggcc cagaatacgc ccgttgtatt acttgatgag ccaaccacct atcttgatat
     7321 caatcaccag gtggacctga tgcggttgat gggcgaactc cggactcagg ggaaaacggt
     7381 ggtcgctgtg ctgcacgacc ttaatcaggc tagccggtac tgcgatcaac tggtggtaat
     7441 ggcaaacgga catgttatgg cgcaaggcac accagaagag gtgatgaccc caggattgct
     7501 gagagcagta ttcagcgtgg aagcggaaat acaccccgag ccggtatctg gcaggccgat
     7561 gtgcctaatg aggtagattg cacaggccgt aagaaccaaa ccacgactga atgaaactgg
     7621 actggcgcca gcaagcctgt tcagactggg gctgaacttt tccggactct gaaagattac
     7681 caatactca
//
LOCUS       DAZFYC010000183         7624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFYC010000183 DAZFYC010000000
VERSION     DAZFYC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.184"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..170)
                     /locus_tag="ACHKW5_004786"
     CDS             complement(<1..170)
                     /locus_tag="ACHKW5_004786"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7513998.1"
                     /translation="MQKNVNPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWR"
     gene            complement(525..959)
                     /locus_tag="ACHKW5_004787"
     CDS             complement(525..959)
                     /locus_tag="ACHKW5_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO7513999.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLLRL"
     gene            complement(947..1348)
                     /locus_tag="ACHKW5_004788"
     CDS             complement(947..1348)
                     /locus_tag="ACHKW5_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7514000.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1608..2177)
                     /locus_tag="ACHKW5_004789"
     CDS             complement(1608..2177)
                     /locus_tag="ACHKW5_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7514001.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            2377..2573
                     /locus_tag="ACHKW5_004790"
                     /pseudo
     CDS             2377..2573
                     /locus_tag="ACHKW5_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2918..3043)
                     /locus_tag="ACHKW5_004791"
                     /pseudo
     CDS             complement(2918..3043)
                     /locus_tag="ACHKW5_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(3395..3661)
                     /locus_tag="ACHKW5_004792"
     CDS             complement(3395..3661)
                     /locus_tag="ACHKW5_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514002.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SVVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3802..4008
                     /locus_tag="ACHKW5_004793"
     CDS             3802..4008
                     /locus_tag="ACHKW5_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7514003.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            4103..4705
                     /locus_tag="ACHKW5_004794"
     CDS             4103..4705
                     /locus_tag="ACHKW5_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514004.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(4611..4808)
                     /locus_tag="ACHKW5_004795"
     CDS             complement(4611..4808)
                     /locus_tag="ACHKW5_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514005.1"
                     /translation="MAQYDDDEHALQVSMRGREHHHFRILSHLPEATGISCGYRQPLC
                     GMPGARTDPSDPSVLRSASDG"
     gene            5906..6823
                     /locus_tag="ACHKW5_004796"
     CDS             5906..6823
                     /locus_tag="ACHKW5_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514006.1"
                     /translation="MVQKILSDKVMNERANAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWIRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCTLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            6908..>7624
                     /locus_tag="ACHKW5_004797"
     CDS             6908..>7624
                     /locus_tag="ACHKW5_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7514007.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHIQEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSACTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVR"
BASE COUNT         1845 a         2003 c         1860 g         1916 t
ORIGIN      
        1 cgccattgtt gtcgccattt gaacaacaga ttggcgttaa tgccattttc aagagcaagt
       61 tttgagatgg atatcccggg ttcacaggag gcagcaacga gctgctgttt aaattcggga
      121 ggataattag ggcagccttt tcgcctgccg ggattcacat ttttctgcat atctgacact
      181 ttggttccca ctacttattt ggtggacacc actttgtcta attcgtcaga ttctgaccag
      241 acggttcagg ctgtacgctt accactattt tatccctgtt gaatttggtt aagccggtgc
      301 agacgagccg ggaaacgcac atctgaaccg gcacaataaa atacaataca accccctgtt
      361 actggagata cgaatggaat gacgaggcag cctgtcagtc acagggggct gttggcataa
      421 aatcaacgga aaggccacca acaccggaat taagctccac agtccccgca ctggcaatcc
      481 attcccggga aaggcattac cgcaaactgc tccggaggca accattacag cctcagtaac
      541 ggattatcag ccctggcatc ttccgacggc acctcctgcc gcccccacgc cctgctgtat
      601 accccggtac cgcagcgtgg gcagcacttt tccccctcat aatcttcgtc acacatcaca
      661 caaaaccact ttctcacctt caccgtataa tccggaaccg tcatgccctg cagatgcaaa
      721 cgacgggcct ctttctctgc cgggtcaagc caggtacatc gcagcaggat tttttcagcg
      781 acagtcatct ggtcatgttc aaaccacggt ggttgtccgt cattgccccg ctgaagtgtt
      841 aacgggcatc cacaggacac acagcacaaa acgtcctgtc ggttgtgctt cgcagcttct
      901 ttcgccgtaa caaaatggcc tttgctgtca ttcgccatat aacagttcag aattcgcata
      961 tttcctcctg agttgtccgg ggaatactga atcctccggt ctggcagtgt gtgcagtact
     1021 gctccccata ataatcgtgg tgacattgtc tgcagtgcca gctggcttta cgcaccacgg
     1081 gtaaggcatc cggtacgaat tgctgcagac gtttaatcaa ctgtatttct ctgcgctccg
     1141 gcctgacata agggcactgt tgaccgtgct ctgtcagcct gtcgtcagtg tgttcaaacc
     1201 agggaagttc agtgtcgtat tgcggatggt atctgagtgc actgccacac aagtggcagg
     1261 tgtagttagc ataaggtgcg gcctgtgcag tacgggcgcc cgtcagacgt ccgttgccat
     1321 caagagcgat aaaggatttt gcgtacatag tcatatacct cagccgccag acgacacgca
     1381 ggcaacagcc gtcccggtac gggcagcgtg gtcagggtgt gaatggcggt tcagttaaaa
     1441 tctggactgg agtttgggta aagcaggctg gttgctgtta cagcactgtg gttgtcacac
     1501 aatggaacac tgacgtaaaa aacagtcagt gggcgagtgg atacatcgtt gccgccagac
     1561 aacaacccat acttttggtt tgccggatat tggcgcgcca gtcgtgctca actccggcta
     1621 cagccaaaat tgcgctcacc ggtgcagtga gctttggtat gctccttcgc cagatagtca
     1681 gcacgttcca gcacctgctg aaagccggtg tcatcattac gcgccagcca caccgccggc
     1741 caggccggaa aatgcaccag cgtggcatgg cacccgacat ccactcccag ggcactgcac
     1801 cacgcctgtt taatcatccc ggccagagac tccggagcgc ggtaatcacc ggcacgacac
     1861 caggtatccc ggttgaccag cagcatcagg tgatagtgtt ttttaccttt gcactcccca
     1921 aactcccggg cccagacgta atgcagggtt gtgggatgca cgcgtttacc ttcccgatgc
     1981 ttacgtttct ggtaagcgtc gattcgggct ttcagggcat tgatgaagcg ggatatcaca
     2041 gctgcgtccg ttgctgccgg tacatccggg agacgcaggt caacccgcaa tgccgtcagg
     2101 cggggatgaa cattcagtgc gtgccgcacc gtctcacgaa tacgttgctg ccagaagggg
     2161 ttgtatttgt aggtcatggt taaatctccg tatggttcat acggaatagc cacgtcgtaa
     2221 aaaatacgca gagtccctga agtgacctcc ggcaggacac tgtctcaggc actctgtgat
     2281 aacccgaacg gtcgtctccg gactgacgat tgaattcccg gcgttgtttt ttttaatgta
     2341 aaaaaccggt tacggataag gcgtggcgag ggagctatcg tgatacgcaa aaagaaatgc
     2401 ggggattgcg gtaattcgat tactcataat acagtgtgtt gcctgtattg tggcgctgtc
     2461 gttccttccg gttattaccg gaagaccgat cgtctgctct gtttactgag ttactgctgg
     2521 tgctgattct ggttaccgtt tcaggcgtca gtgtatttgt actgttgcag taggttactg
     2581 gggagggatt atcaggaatc ggcggagcag agcgactcgc tgcgtttgtt gtgccagaca
     2641 gtaacgcacc ggatccctgc aaaatggtac ctgaatcaat cacagttagg tattccgcca
     2701 atccgtgatc gaatcgagcg gaatacgcag ccccgacaaa tacgagcccc cttcagcaga
     2761 tacaggccgg taatacctga aaatcagcct attaccgccc acgcctccgg caccattccc
     2821 ttttgatttc gaattctttg ttgtcgctat accgcttaat gttcccggca cctccggtgc
     2881 agataacacc ggagtcatcg tagcgctgct tcaatattca ggtgacatat ttccagacgg
     2941 aagccggcgc gcgatcgtaa agtttgttca ttgtcagttc tgccagccgg ttgtcaacgg
     3001 cagagttaaa ggcttccagc tccgcaaggg taagtttata acgcgtgtgg gaattcactt
     3061 ttcccgcgtc tcctgacgta ttacgggctg ccacttacca ggtgtcccgc aatacagcct
     3121 tagtgttccg ttatcgggga tccggacgca ccgcaacaac cggggaagta tttctggttt
     3181 agagcagtgt caagagggat ttctcagccc ctcactcacc ggctcagtct cagggcagga
     3241 gcattaatac gctcagttca tgtcttatac aggaatgggc acagggggag ctggcgtgtc
     3301 actgctgatt ttacgcatca gcgttcatgt atctgcactc tgatacatga acgattcaca
     3361 gcgctctgtt accggagaga tttactctct tagcttactc tggcaaatcc tttacgttac
     3421 tctctgatga cttcttatat ttataatacc aatcagacag ccttatccca tcggtgatat
     3481 cagtctcctc ctcgccagga acgggctgac tcactgtaac caccaccgaa aacaactctg
     3541 caattatcag aacgcctgct tctctccctg tcctcacgaa aactatcccc tctttatcgc
     3601 gcgtgcgtgc ggaagcatct tttcgcaaca accacccggt ataccggtac ggctcagcca
     3661 ccgcaacctc cccgtttatc tcctggcggt cacattcccg attatttttt acgtttctcc
     3721 ccggttgtta tgccggtgaa ggtggcgcgc tgttttcatg accacaccgg ctggtttaac
     3781 aacatccgga ggaacattct catggctacc ccagtttcac tgatggatga ccagatggtc
     3841 gacatggcat ttatcactca actgaccggc ctgacagata agtggtttta caggctcatc
     3901 agggatgggg cctttccggc tcccatcaag ctgggccgca gctcccgctg gctgaaaagt
     3961 gaagtggaag cctggctgca ggcgcgtatt gcacagcccc gcccgtaatt tctgaccgtt
     4021 atccgttcac ccgcagcaga cgtaatcccc ggcctgccgg cggcattctg ctgcctgtta
     4081 tatccccgtg aggaatatga aaatgaaaca acagtatcag acccgctacg aatggctcca
     4141 cgaaagctac cagaaatggc tgaccggctt cacccggcac gccgtatcct ggggcgtgtg
     4201 tcatccgaat atctactact tccataatct gacgcccggt tgggtgtcat tcaatggtga
     4261 aaaaccggag attgccatcg taccccagag cctgcaccgg ctgatttatg gccctgacaa
     4321 acgggccacg ccgcccctgg atgatgatct gattgtgaat ttatgcacca gtgaacatct
     4381 gctggttcat catccgatgc tggaaggcat tctcctgtct gaatgtgaac gtctgaggca
     4441 gcgttctctg gcaaataaac tgatcagcct cttccgtcag tttggtggca cggagttgcg
     4501 cctcaaactg gtctggcttt gctggcttga tttaatgacc ggaaacagcc ttgaagactg
     4561 gaaagagaac ctgaaacgga aatcagaaaa agagctggag gaatggatca tcaaccgtca
     4621 gaggcagagc gcagcactga cggatctgat ggatcagtac gtgctcctgg cataccgcac
     4681 aacggttgac gatagccgca actgatgccg gttgcttccg gaaggtgtga caaaatacgg
     4741 aaatgatgat gctcccggcc ccgcatgctc acctgcagag cgtgttcgtc gtcatcatac
     4801 tgagccatga cgggcgtctt aagggcatcc tgtcattgct gagcaggcaa caccaccaga
     4861 ggagccacgg acagggatgt tctttacagc ccgatacacg ccaggttctc agcagactga
     4921 atgtgcaggt gcggttattc accagaaatg gcatgctgcg gtacataccc atcctggtga
     4981 caatatgtac gtggcgacag cgatcccggc actgtggaag agactcactc agcctgcagg
     5041 atattctttc cggcatccgg gaatgtccgc ctgaatgcaa acaggatgat atttgaatcc
     5101 gtaatactgt tctttcatca taaataattt atgcagatac agttaaccgt tacagtagcc
     5161 ggtttgtttt accgggaaat aatatccgtc agactgatgc cggaacatat ctgaaacacg
     5221 ttatgatgtt ttgttatcag aaatgttgta tattcttatc cgtggcgaca gtttaatgcc
     5281 ctctggtatc acctgataat gcatatcctg cgatgattcg ttatatttac gggacacgcc
     5341 tcttttcatt gtgctccttt tcagacttca ttttccgtaa gtgttccccg gcataaagga
     5401 ggtacctttc tggcgtttat aactctcttt atcggtgacg ccggtaatac agggaatacc
     5461 acaccgacaa acgaggtatt aacagacgat atttcagtgt ccataatttt tctgtccggt
     5521 taaataacgt cagtctgctg ccctctgact gtaatcatca aagccggaat gcataccggg
     5581 ttacaacgta aaaaaagtga tgctggcccc cggtgcagct ggcgggcgaa acatgtttac
     5641 ctttcaatac cccctgtctg cagtctccac tcttccatcc tcttaagcta cgtcggccac
     5701 atgagtgcac ttccatgata acaggtattc ctgtcagcat aacgaagccg gttaccttcc
     5761 cttcctcctg catttcctgt cagaatgact gtacagcgcg cagattgcct ttttattgac
     5821 caccttaaca taccattata aacttctttt tatcatgtta ttatcacctt attatgttta
     5881 attttcaagc aaccggagaa agaggatggt tcagaaaatt ttgtcggata aggttatgaa
     5941 tgaaagagca aatgcgtatt actcttatta tcttggagag cgtaatatct ccgtactgcc
     6001 ccttaatgtt tatgatcccc cggaacgttt tatcgcttac ataaaaaaaa acagagaaaa
     6061 tctgaacatc acactttcag attttgaact tgaacaaatc atttctggta tgcgtctgaa
     6121 ggccctggcc ttcctggtcc ccctggaaaa aatatcctgg atcgcgggca gtgaacgcgc
     6181 atgcttattt tcctggtatc tgttgatgca gtttatccag aataacaggg caaaaataag
     6241 cgcagacttg cttcaaaaga ataaactgta tcttaaagaa gagtatctgg aagggaatgc
     6301 cttcccgtcg gactcatcga cacagttcag acaaatactc agagttctcg atattctttc
     6361 tgataagaat ttgcgggatg agtggataat ccagaccaaa gacagatgga tacgggcatt
     6421 taaatcaaag agtcctttca gttacctcct cccggagaac gaacatgaat gcatatggac
     6481 atggaattat ttgaagggaa aaaatattgc actggaaaaa ctggccagtt ttcctggttc
     6541 tgctgatatt taccacgcaa ttcacctttc atttgacata tgggttacct gtacactcac
     6601 ctcacctgac gatataaaaa atttcagaaa cagtttcaat aaggccaagg cgcagcgcaa
     6661 gtacaaaaaa atgcaggaag ataaagtcaa tgtacagttt tttcttgacg cagaaaccag
     6721 ggcgcagctc aaagaattat cgagggtcag aagactcagt accggcgaaa tgctgcatga
     6781 cctgattgtt gaagagtata aaaggtaccg acattccaga taatttcaga catatattat
     6841 ctctgtgagt cccccgccac ctttccggtg tgcgggggtt ttgtcttttt tcaccgggaa
     6901 tacatgtatg aatccgtctg atgccattga ggcaattgaa aaaccgctct cctccctgcc
     6961 ttactcactt tcccgtcaca tccaggaaca tctgcgcaaa ctcaccagtc acgaacccgt
     7021 gattggcatt atgggtaaaa gcggggccgg taaatcctca ctctgtaatg cactgtttca
     7081 gggggaggtc accccggtca gtgatgttca cgccggcacc cgggaagtgc ggcgcttccg
     7141 tctgagtggc cacggtcaca gcatgattat cactgacctg cccggggtgg gcgagagccg
     7201 ggacagggat gcagagtatg aagccctgta ccgtgacatt ctgcctgaac ttgacctggt
     7261 actgtggctg attaaagccg atgaccgtgc cctgtctgtg gatgagtatt tctggcgaca
     7321 catcctgcac cggggacatc agcaggtgct gtttgtggtg acgcaggccg acaaaacgga
     7381 gccctgccat gaatgggata tggccggcat tcagccttct cccgcacagg cacagaacat
     7441 tcgtgagaaa acggaggcgg tattccggct gttccggccc gtacatccgg ttgtggccgt
     7501 atcggcctgc accggctggg aactggatac gctggtcagt gcgctcatga cagcgcttcc
     7561 cgaccatgcc gccagtcccc tgatgacccg cctgcaggac gagctgcgca cggaatctgt
     7621 ccgg
//
LOCUS       DAZFYC010000184         7561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFYC010000184 DAZFYC010000000
VERSION     DAZFYC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.186"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..695)
                     /locus_tag="ACHKW5_004798"
     CDS             complement(<1..695)
                     /locus_tag="ACHKW5_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO7514008.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRW"
     gene            complement(706..1707)
                     /gene="ltaE"
                     /locus_tag="ACHKW5_004799"
     CDS             complement(706..1707)
                     /gene="ltaE"
                     /locus_tag="ACHKW5_004799"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO7514009.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1744..3462)
                     /gene="poxB"
                     /locus_tag="ACHKW5_004800"
     CDS             complement(1744..3462)
                     /gene="poxB"
                     /locus_tag="ACHKW5_004800"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO7514010.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3595..4563)
                     /gene="hcr"
                     /locus_tag="ACHKW5_004801"
     CDS             complement(3595..4563)
                     /gene="hcr"
                     /locus_tag="ACHKW5_004801"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO7514011.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4575..6227)
                     /gene="hcp"
                     /locus_tag="ACHKW5_004802"
                     /gene_synonym="priS"
     CDS             complement(4575..6227)
                     /gene="hcp"
                     /locus_tag="ACHKW5_004802"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO7514012.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6371..7270)
                     /gene="lysO"
                     /locus_tag="ACHKW5_004803"
     CDS             complement(6371..7270)
                     /gene="lysO"
                     /locus_tag="ACHKW5_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO7514013.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         1825 a         2057 c         1992 g         1687 t
ORIGIN      
        1 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
       61 acctctggtc cggcggcttc gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc
      121 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
      181 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
      241 cctgcgccaa cgataattcc ggcccgaagt tcggtcacag gtacattcgc ttcacgaaga
      301 atgtccgccg tagcctgacg agcacgcaga tgatccgact gctcatgtgg cggggcctgc
      361 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
      421 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
      481 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
      541 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
      601 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
      661 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
      721 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
      781 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
      841 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
      901 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
      961 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
     1021 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
     1081 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
     1141 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
     1201 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
     1261 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
     1321 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
     1381 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
     1441 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
     1501 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
     1561 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
     1621 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
     1681 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
     1741 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
     1801 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
     1861 aatggctaac tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
     1921 ggcgcgttgc agggcttcat caacttcaga cgctttttct acacggatac ccgtaatgcc
     1981 gcacgcttcg gcaatgcggg caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata
     2041 gccaccagct ttcatctcca tcgccacaaa gcccagcacg ctgttgttaa agacgacaat
     2101 tttcactggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
     2161 gccatcgccg cacatggcga ccacctgacg ttctggctct gtcgcctgcg cacccagcgc
     2221 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
     2281 gttcattttt agataacgtg ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat
     2341 agcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
     2401 ctcgctcggt ttagctaaat cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc
     2461 tttatccaga aacttgcgat cggctttttc ttccaccaat ggaagcaatg cacgcagagt
     2521 cgacttgata tcgccgacca gtgccatatc caccttgctg tgagcgccga tgctggctgg
     2581 gttgatatca atctgaatga ttttggcatc ggtcgggtag aaggcgcggt agggaaattg
     2641 cgtgccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaagcc
     2701 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgtt ctttaccgcg
     2761 cagggcatga acaataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
     2821 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
     2881 cagtttgcgt aactcttctt cttccggcgt cacgactggt tgtggcgcat gataccagtg
     2941 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cctggtaaca cgacaaccga
     3001 aacgccacgg ttaagcaccg ctttgcgcat ggcaatcgcc agtacttgtg ggatctgctc
     3061 cgggctggaa accaactcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
     3121 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
     3181 cggaacgtga ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccggggcc
     3241 gcacgatccg gcgcagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa
     3301 ggccgccact tcttcgtggc gggtggacat ccactcgatg gtgcccatgc gattaagact
     3361 gtcactaaga ccgttcagag agtcgccagt gactccccag atgcgtttca cccctgccga
     3421 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
     3481 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
     3541 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
     3601 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
     3661 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
     3721 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
     3781 cgcctccagt agtgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
     3841 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
     3901 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
     3961 gcaggtcatc acggtacgtg aagctaagtc aggtacacct gccagcagtt cgcgagtgag
     4021 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
     4081 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
     4141 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
     4201 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
     4261 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
     4321 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
     4381 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
     4441 ttgcccggcg cgatatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg
     4501 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
     4561 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
     4621 gaacgcaggc caaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
     4681 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgacg
     4741 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt gacaccgcag
     4801 cccagtttct ctgccagagt gacagccaga ataatcgctg agtaagcatc gttacattga
     4861 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
     4921 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
     4981 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
     5041 agtttttcac ggctcaccag atcaatcagc gtatcagcag cgccaagcag cgtctggcga
     5101 ccaaaaccca cggtgatcag gtgcggaatt tcgctgtacg ggaagcccgc catttgttgc
     5161 gcctgggcga tgaccgcaga aaaatcttcg ccatccagat gacgcacacc cggccagcca
     5221 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
     5281 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
     5341 ccactgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
     5401 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
     5461 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
     5521 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
     5581 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
     5641 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
     5701 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
     5761 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
     5821 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca
     5881 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgcgc tttcagcgcc
     5941 tcgcgcaggg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
     6001 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
     6061 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
     6121 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
     6181 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
     6241 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
     6301 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
     6361 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
     6421 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
     6481 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
     6541 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
     6601 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
     6661 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
     6721 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
     6781 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
     6841 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
     6901 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
     6961 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
     7021 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
     7081 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
     7141 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
     7201 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
     7261 cagaaaacat gaaaacccag tccttgcaaa gatgaagtcg aaatgcgcga tgacacacta
     7321 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca taaggagcag
     7381 tagagtattc gttttcattt aaagatattc ttgcgcttta attacaaact gcaccgatgt
     7441 tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg
     7501 gctgagggcg cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata
     7561 a
//
LOCUS       DAZFYC010000185         7485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFYC010000185 DAZFYC010000000
VERSION     DAZFYC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.187"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            380..895
                     /gene="ehxC"
                     /locus_tag="ACHKW5_004804"
                     /gene_synonym="hlyC"
     CDS             380..895
                     /gene="ehxC"
                     /locus_tag="ACHKW5_004804"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO7514014.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            897..3893
                     /gene="ehxA"
                     /locus_tag="ACHKW5_004805"
     CDS             897..3893
                     /gene="ehxA"
                     /locus_tag="ACHKW5_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO7514015.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3943..6063
                     /gene="ehxB"
                     /locus_tag="ACHKW5_004806"
                     /gene_synonym="hlyB"
     CDS             3943..6063
                     /gene="ehxB"
                     /locus_tag="ACHKW5_004806"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO7514016.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            6067..>7485
                     /gene="ehxD"
                     /locus_tag="ACHKW5_004807"
     CDS             6067..>7485
                     /gene="ehxD"
                     /locus_tag="ACHKW5_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO7514017.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETIN"
BASE COUNT         2411 a         1150 c         1657 g         2267 t
ORIGIN      
        1 gtttgctttt ctgtgtaggt ttagattttg tgtttgcagg gtaaacaaaa ctgattaact
       61 cgtcatttcg gtaactgaaa tagtttgctg tattctgttt acaatctctt taataaggca
      121 tttgtcacgt ggctattcat atgaaaatca tacgttatct tacaaatcaa tcatctgagt
      181 gttataatat aacttagctg tgatatgtgt aagaatgttt aggcaatata ttattatcgc
      241 taatgttgtt ttggttggga gagattctcc ttgcggtagc tttttgtttt tagatgcttc
      301 ttgcttaaaa agatataatt tctgttcttt tatataggat tctttacatt tactgagttt
      361 tcttatttat ttaaacatta tgaaatctaa tgcttttgac gtcatgggga aggttgcatg
      421 gctatgggcc tgttctcctc tgcataaaaa atggccatta tctgtttttg ctattaatgt
      481 catacctgct attcagacaa atcaatttgc tctcttaata aaggatgagc ttcctgtagc
      541 tttttgtagc tgggccagtt tagatctgga atgtgaggta aaatatataa atgatgtaac
      601 atctttgtat gcaaaagact ggatgtcggg agaaagaaag tggtttattg actggatagc
      661 tccatttgga cacaacatgg agttatataa atacatgagg aaaaagtatc cttatgagtt
      721 attcagagca ataaggttag atgagagcag taaaacagga aaaatagctg aatttcatgg
      781 aggggggata gataaaaaac tggcatcaaa aatattcagg caataccatc atgaacttat
      841 gtctgaagta aaaaacagac aagattttaa ttttaatatt gagaaagaaa actaatatga
      901 cagtaaataa aataaagaac attttcaata atgcgacatt gactacaaaa tcagcattta
      961 atacagcatc atcaagcgta cgttccgctg gcaaaaaact catattatta atacctgata
     1021 attatgaagc tcagggcgtg ggtattaatg agttggtcaa agctgctgat gagcttggaa
     1081 tagaaataca ccgtactgaa cgagatgata cagcgattgc aaaccagttt tttggtgcag
     1141 cagaaaaagt tgtaggatta actgaacgtg gtgttgcaat attcgcacca caacttgaca
     1201 aacttctgca gaagtatcag aaagttggga gtaaaatagg aggaaccgct gaaaatgtag
     1261 gtaataatct gggaaaagcc ggaacagttc tctcagcact acagaatttt acggggattg
     1321 ctttatcagg catggctctt gatgaattgc tgagaaaaca acgggaagga gaggatataa
     1381 gtcagaatga tattgccaaa agtagtattg aacttattaa tcagcttgta gatacagtat
     1441 caagtataaa cagtaccgtt gattcatttt ctgagcagct taaccagctt ggctcatttt
     1501 tatccagtaa acctcgctta agttctgttg gtgggaaatt acaaaattta ccagacctgg
     1561 gctccctggg ggatgggctg gatgttgtct ccggaattct ttctgctgta tcagcaagct
     1621 ttattctggg aaacagtgac gcacatacag gaacaaaagc tgcagcgggt atagaactga
     1681 caactcaggt tcttggaaat gttggtaaag ctgtttcgca atatattctg gctcagagaa
     1741 tggcacaggg gttatcgaca acagctgcaa gtgcgggtct gatcacatcg gctgttatgc
     1801 tggctatcag tcctctttct ttcctggctg ctgcagataa atttgagcga gctaagcagc
     1861 ttgaatcata ttctgaacga tttaaaaaat tgaattatga aggggatgct ttactcgcag
     1921 cctttcataa agaaaccgga gctatagatg cagccctgac aacaataaat actgtcctga
     1981 gttctgtatc tgcgggagtt agtgcagcct ccagtgcatc cctcataggg gccccgataa
     2041 gcatgctggt gagtgcatta accggtacga tatctggcat tctggaagca tcaaaacagg
     2101 ctatgtttga gcacgttgca gagaaattcg ctgctcggat caatgaatgg gaaaaggagc
     2161 atggcaaaaa ttattttgag aatggctatg acgcaagaca tgctgcgttt ttagaagact
     2221 ctctgtcttt gcttgctgat ttttctcgtc agcatgcagt agaaagagca gtcgcaataa
     2281 cccagcaaca ttgggatgag aagatcggtg aacttgcagg cataacccgt aatgctgatc
     2341 gcagtcagag tggtaaggca tatattaatt atctggaaaa tggagggctt ttagaggctc
     2401 aaccgaagga gtttacacaa caagtgtttg atcctcaaaa agggaccata gacctttcaa
     2461 caggtaatgt atcaagtgtt ttgacattta taacaccaac atttacccca ggagaagaag
     2521 ttagagaaag aaaacagagt ggtaaatatg aatatatgac atctcttatt gtaaatggta
     2581 aggatacatg gtctgtaaaa ggcataaaaa atcataaagg tgtatatgat tattcaaaat
     2641 tgattcagtt tgttgaaaag aataacaaac actatcaggc gagaataatt tctgagctcg
     2701 gagataaaga cgatgtggtt tattctggag caggctcatc agaagtattt gctggtgaag
     2761 gtcatgatac cgtatcttat aataagacgg atgttggtaa actaacaatt gatgcaacag
     2821 gagcatcaaa acctggtgag tatatagttt caaaaaatat gtatggtgac gtgaaggtat
     2881 tgcaggaagt cgttaaggaa caggaggtgt cagtagggaa gcgaacagag aaaatacaat
     2941 atcgtgattt tgaattcaga accggtggaa ttccttatga tgtaatagat aatcttcatt
     3001 ctgttgaaga gctcattggc ggaaaacatg atgatgaatt caaaggcggt aagtttaatg
     3061 atatattcca tggcgcagat gggaacgatt atatcgaagg taattatggt aatgatcgac
     3121 tatacggcga tgatggggat gattatatat ccggaggaca gggagacgac cagttatttg
     3181 gtggtagtgg aaacgataaa ttgagtggag gggatggtaa taattatctg acaggaggaa
     3241 gcggtaatga tgagcttcag gcacacggag cttataatat tctgtcaggt ggtactggtg
     3301 atgataaact ttatggtggt ggtggtattg atcttctgga tggaggggaa ggtaatgact
     3361 atctgaatgg tggttttggt aatgatattt atgtttatag gcaaaactat ggtcatcata
     3421 caattgcaga tgaaggaggt aaaggagatc gtttgcactt atctgatatt agctttgatg
     3481 atatcgcatt taagagagtt ggaaatgatc ttatcatgaa taaagccatt aatggtgtac
     3541 tttcatttaa tgagtcaaat gatgtcaatg ggataacatt taaaaactgg tttgcgaaag
     3601 atgcctcagg agcagataat catcttgttg aggttataac agataaagat ggtcgagaga
     3661 taaaagttga taaaatacct cataataata atgaacggtc aggttatata aaagccagta
     3721 atatagcatc tgaaaaaaac atggttaata tcaccagtgt tgccaatgat attaataaga
     3781 ttatttcttc agtttcagga ttcgattcag gtgatgaacg attagcatct ttatataatt
     3841 tatccttaca tcaaaacaac acacactcaa caactttaac gacaactgtc tgagactaaa
     3901 agaggaggtg atggccattc caggaggaat ggtcatcgtt atatgatgag taaatgtagt
     3961 tctcataata gtctgtatgc actgatattg cttgcacaat atcataatat aactgtcaat
     4021 gctgaaacta taaggcatca gtataatacc cacacacaag attttggggt gactgaatgg
     4081 ttactggcag cgaaatccat tggcttaaaa gcaaaatatg tagaaaaaca tttttccaga
     4141 ttgtcaataa tttctttacc tgcgttgata tggcgggatg acggtaagca ttatatattg
     4201 tctcgtgtta ctaaagattc atcacgctat cttgtttatg atccagaaca acatcagtca
     4261 ctaactttta gtcgggatga gtttgaaaaa ctgtatcagg gaaaagtcat tctggttacg
     4321 tcaagagcaa cagtagtcgg agagttagct aaatttgatt tttcttggtt tatcccctct
     4381 gttgtgaaat acaggaggat tttacttgag gtgttaactg tttctgcttt tattcagttt
     4441 cttgcgttaa taacacctct tttttttcag gttgtaatgg ataaggtttt agttcaccgg
     4501 gggttttcaa cgttaaatat tatcacaata gcatttatta tagtgatact ttttgaagtg
     4561 atattaaccg gagccagaac ttatattttc tctcatacta caagtcgtat tgacgtcgaa
     4621 ctgagcgcta agttattcag acatttgctt gcattgcctg tttcatattt tgaaaatcgc
     4681 agggtcggag agaccgttgc cagagtaagg gaactggagc aaattcgtaa ttttttaacc
     4741 ggacaagcgt tgacatcagt tcttgatcta tttttttctg taatattttt ttgtgtcatg
     4801 tggtattaca gccctcaatt aacactggtt atattattgt cactaccttg ttatgttata
     4861 tggtcattgt ttatatcacc cttattacgt cgacgtcttg atgataagtt tctcaggaat
     4921 gcagaaaatc aagcttttct tgtcgaaacg gtaacagcaa taaatacaat taaatccatg
     4981 gcagtatcac cacagatgat tgctacatgg gataaacaac tggccgggta tgttgcttcc
     5041 agtttcaggg taaatctggt tgcaatgaca gggcagcagg ggatacagct gatacagaaa
     5101 agtgtaatgg taattagtct atggatggga gcacatcttg tcatatcggg agagataagc
     5161 atcgggcagt taatcgcgtt taatatgctt gcaggtcagg tcattgctcc tgttatcaga
     5221 ctggctcatc tttggcagga ttttcagcag gttggtattt ctgttgagcg ccttggtgat
     5281 gtattaaata caccggtaga gaaaaagtca ggcagaaata tactgccgga aattcagggg
     5341 gatatcgaat ttaaaaatgt cagatttcgg tattcttctg acggtaatgt tattttgaat
     5401 aatattaatt tatacatatc aaagggggat gttatcggta tagttggccg ttctggttca
     5461 ggaaaaagta cattaacaaa actgcttcag cgcttttata taccagagac cggacagatt
     5521 ttaattgatg ggcatgattt atcacttgca gatccagaat ggttacgacg ccagattggt
     5581 gttgtattgc aggaaaatat actattaaat cgtagtatta tcgataatat tacattagct
     5641 tctccggctg tatctatgga acaggctatt gaggcagcca gacttgcagg tgcccatgat
     5701 tttattacag aactaaaaga agggtacaat actattgttg gagaacaggg cgttggtctt
     5761 tcaggagggc agcgccaacg gatcgcaata gcccgggctc ttgttacaaa tcctcgaatt
     5821 cttatttttg atgaagcaac cagtgctctt gattatgagt cggaaaatat aataatgaaa
     5881 aatatgtcaa gaatatgtaa gaacagaacc gtaattatta ttgcacacag gttgtcaact
     5941 gtaaaaaatg caaacagaat aattgttatg gataatggct ttatttctga agatggcaca
     6001 cataaagagc ttatctccaa aaaagacagt ttatatgcat atttatatca gttgcaggca
     6061 taacgtatgc gcttttacat gaaaggttta tgggatttgg tttgccgtta taagacggtt
     6121 ttttctgatg tctggaaaat tcgtcacaca cttgatgctc ctgtgagaga aaaagatgag
     6181 tatgcatttc ttcctgctca ccttgaactc attgaaacac cagtatccag acgttctcat
     6241 tttgttgtat ggagtatttt attatttgta attatatctc ttctcttatc tgttcttggg
     6301 aaagttgaag tggtttcagt agcaaatggt aagtttactc atagtggaag aagcaaagaa
     6361 ataaaaccga tagagaatgc gattgtagaa aaaataatgg taaaagatgg ctcttttgta
     6421 aaaaaaaatg atccattagt tgaattgacg gtgcctggtg ttgaatctga tattttaaaa
     6481 tcagaagcat ctttgttgta tgaaaaaaca gaacaatatc gctacgcaat tctttctgaa
     6541 tcaatacaac ggaatgagct tcctgaaata aggataacag attttcctgg cggagaagat
     6601 aatgcgggag gtgaacattt tcagagggtt agctcattaa taaaagagca attcatgaca
     6661 tggcagaaca gaaaaaatca aaagcaactc acattaaata aaaaaatagt tgaacgggat
     6721 gcagcgcttg ctcgtgttag cctgtatgag catcaggtat cacaggaagg aagaaaactc
     6781 aatgatttta agtatttgtt gaataaaaaa gctgtttctc aacattcagt tatggagcag
     6841 gaaaatagct atattcaggc aaaaaatgaa catgcagtct ggcttgcaca ggtttctcaa
     6901 cttgaaaaag aaatagaact tgtgcgggaa gaactggcac tggagacgaa tatcttcaga
     6961 agtgaaatta tcgagaagca cagaaaatca acagataaca ttgtgttgct ggagcatgag
     7021 cttgaaaaaa acaggcagag aaaagcatca tcttttatta aagctcctgt gagtggtact
     7081 gttcaggagt taaatataca tacagaaggt ggtgttgtca caacagcaga aacgctgatg
     7141 attattgtcc ctgataatga tattctcgaa gtaacagcat ctgtacttaa caaggatatc
     7201 ggttttatac aacctggaca ggaggtcgtt attaaagtag atgcatatcc atatacacgt
     7261 catggttacc ttacagggaa agtaaaaaat ataactgcag attctgtttc tgttccggat
     7321 acagggcttg tatttaacgt gattatatct gttgatcgga atgatataca gggagaaaga
     7381 aaaaaaatcc ctgttacagc tggaatgact gttatggcag aaataaaaac cggggtgcgt
     7441 agtgttatca gctatcttct tagtccatta aaggaaacta ttaat
//
LOCUS       DAZFYC010000186         7323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFYC010000186 DAZFYC010000000
VERSION     DAZFYC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.188"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(204..623)
                     /locus_tag="ACHKW5_004808"
     CDS             complement(204..623)
                     /locus_tag="ACHKW5_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO7514018.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
     gene            complement(707..1144)
                     /locus_tag="ACHKW5_004809"
     CDS             complement(707..1144)
                     /locus_tag="ACHKW5_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFO7514019.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGR
                     REFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
     gene            1402..3495
                     /gene="cda"
                     /locus_tag="ACHKW5_004810"
     CDS             1402..3495
                     /gene="cda"
                     /locus_tag="ACHKW5_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFO7514020.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPA
                     AGNNVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGS
                     GVSPLYISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     KQPPVYTQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGM
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDA
                     GGLRDFIYWRPDATGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTNNVVILKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            3492..3755
                     /locus_tag="ACHKW5_004811"
     CDS             3492..3755
                     /locus_tag="ACHKW5_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFO7514021.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKNLSQDV
                     LNCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
     gene            3825..3971
                     /locus_tag="ACHKW5_004812"
     CDS             3825..3971
                     /locus_tag="ACHKW5_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO7514022.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTG
                     VAIQ"
     gene            complement(4200..4313)
                     /locus_tag="ACHKW5_004813"
     CDS             complement(4200..4313)
                     /locus_tag="ACHKW5_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO7514023.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDFTPLAAAIGN"
     gene            complement(4983..5174)
                     /locus_tag="ACHKW5_004814"
     CDS             complement(4983..5174)
                     /locus_tag="ACHKW5_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO7514024.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            5241..5564
                     /locus_tag="ACHKW5_004815"
     CDS             5241..5564
                     /locus_tag="ACHKW5_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO7514025.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5773..6207
                     /locus_tag="ACHKW5_004816"
                     /pseudo
     CDS             5773..6207
                     /locus_tag="ACHKW5_004816"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6260..6802
                     /locus_tag="ACHKW5_004817"
     CDS             6260..6802
                     /locus_tag="ACHKW5_004817"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO7514026.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRD
                     AISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVT
                     GGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSG
                     VKGENNWTVEKKNAVRLVRE"
     gene            6809..7042
                     /locus_tag="ACHKW5_004818"
     CDS             6809..7042
                     /locus_tag="ACHKW5_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO7514027.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
BASE COUNT         1815 a         1616 c         2067 g         1825 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagggatt atggtaaatc cacttactgt cttccctcgt agccatcgag ataaaccgca
      241 gaaatcgtct cagccagcag cctggatttt ttcggggtaa ggtctttgct gcggtaatca
      301 gtacccgcaa attgagttgt gccccggagt gagtttaaaa attcctggct gtgaaactgc
      361 tgaatgcgtt gctgcacatc actccgtgat tttcctgtcg ctttatcctt tgctcccata
      421 tcccagaact gccggaaaaa aggcaccatc attcgtgttg tgtcggcctt ctgggtggaa
      481 aagttaggat caaaaccatc gtctgatgca taaacgtaat gccgggtatg ttgttccaca
      541 ctgccctttg gcgtgcaggc tgtcaggacc gttgccgctg tcagcagggc gataagatac
      601 agtgtgccgc cggaacgtct catgagtgtc tccttacctt taaatctttg tactcagtga
      661 cttatattta accgctgact atcttatcat cacttgcagc cgatgctcac cggctgaaca
      721 gtctggccca gaatccccgt tttttttcct gctccagtgc ctgcctgagc tgctctatct
      781 ctttatgtag ctgttcccgt tcggcttcct ggcgccggcg ttccgtatcc tgtgcctgtt
      841 tgtcctcaag catcagtgtc aggcattgtt tcagctcatt cagctcccgg agaatgcgtt
      901 ctgtctgctg atcatgtgga ttttctgcct gtcccgcact gtgcctttct gctgtctcat
      961 cctgctttaa ttcgccgtat gcccggatca gttcactggt ttcaaactcc cgtcgtccgt
     1021 cctggccggt acggtaactg acaaggcctg cgcgcatatc acggtaaaac tgtgaacgcg
     1081 atctgcctgt cattttcagt gcgtcctgga gtgtgtgcca tgccatgaag tgacagtgtc
     1141 ccattggtgt ctcatatcat ggtttcagta aaacatactg gggccggaga acggtaatgt
     1201 ttgtgctggt ttttgtggca tcgggtggga atagcacgtg gtgtgggtgg ctgttttttt
     1261 gatcgttttc acaaaaacga aaatccacag ggttgacagg gaaaacgcag cggcgtaatt
     1321 ttaaactgta tataaaacca gtggttgcat atacagtatg tgtcttttaa ctatttgttt
     1381 ttaatgtcaa agaggttttc tatgagtgat aatgaaggta gtgtcccgac agaaggtatt
     1441 gattacgggg acacaatggt tgtgtggccg tcaactggac gaattccggg cggtgatgtg
     1501 aaacccggtg gttcatcagg tctcgctcca tccatgcctc cgggatgggg agattacagt
     1561 ccacaaggta tcgcacttgt acaaagtgtt ctttttccag gaattattcg ccggattatc
     1621 cttgataagg aacttgaaga gggggactgg tcgggatggt ctgtcagtgt gcatagtccc
     1681 tggggaaacg agaaagtttc tgctgcacga acagttcttg agaatggttt acgtagtggt
     1741 ttgccagaac catcacgccc ggctgctgtt tcttttgccc gtctggagcc tgcttccgga
     1801 aatgagcaaa aaattattcg tcttatggtt acacagcaac tggagcaggt aacggatatt
     1861 cctgtcagcc agttaccagc agcgggtaat aatgtaccgg tgaaatatcg tctgacggac
     1921 cttatgcaga acggtacgca atatattgct attatcggag gtattccgat gacagtgccg
     1981 gtagttgatg ccgttccggt tccggaccgg agccgtccgg gaacaaatat taaagatgtt
     2041 tacagtgccc ctgtatcacc aaatctaccg gacctggtat taagtgtggg gcagatgaat
     2101 actccggttc tgtctaatcc cgaaattcag gaagagggcg ttattgctga gacaggtaat
     2161 tatgttgagg caggttatac gatgtccagt aataatcatg atgtcattgt tcgttttcct
     2221 gacggcagcg gtgtttctcc attatatatt tcaactgtgg agattcttga ccgtaatggt
     2281 ctgagtcagc gtcaggaagc cgaaaataaa gcaaaggatg atttcagagt caagaaagaa
     2341 gaagcggtag cccgggccga agctgaaaaa gcaaaagccg agttatttag taaagcaggt
     2401 gtgaaacagc ctcctgtata tacacaggaa atggtggaaa gggctaattc agtaatgaat
     2461 gagcagggaa ctcttgttct gaataacact gccagttctg tacaactggc gatgaccgga
     2521 actggtgtct ggacggctgc tggtgatatt gcaggcaaca taagcaagtt tttcagtaat
     2581 gctctggaga aagtaaccat acccgaagtg ggtcccctgc ttatgcggat ttctcttggc
     2641 gctctgtggt tccattcaga agacgccgga gcaggaagtg atatcgtgcc ggggcgagat
     2701 atggaggcta tgttctcgct gaatgctcag atgttggcag gacagggcat ggtcattgaa
     2761 cctggtgcta cgagtgttaa tctgcctgtt cgtggacaat tgataaacag taacggacaa
     2821 cttgctctgg atttactgaa gacagggaat gatagtatcc ctgctgcggt tcctgttctt
     2881 aatgctgttc gtgatacagc tacaggactg gataaaatta cgctgccagc agtagtcggt
     2941 gcaccttcca ggacgattct ggtaaatccg gccccacaac cttcggcacc aacagataca
     3001 ggaaatcatc aacccgttcc ggttacccca gtgcatacag gtacggaggt aaaaccggtt
     3061 gaaatgccgg taacgacgat tactccagtc tctgatgccg gtggacttcg agattttatt
     3121 tactggcgtc ctgatgctac cgggactggt gttgaagctg tctatgtgat gcttaatgat
     3181 cctctggatt cagggcggtt ttcacgcaaa cagcttgata aaaaatataa acatgctggt
     3241 gattttggta ttagtgatac taaaaagaat cgtgaaactc ttactaaatt cagggatgct
     3301 attgaggaac atttatccga taaggataca gtagagaaag gaacataccg acgagaaaaa
     3361 ggatcaaaag tttattttaa tcctaataca aataatgtgg tgatactaaa atcaaatggt
     3421 gagttcttat ctgggtggaa aataaatcca gatgcggata atgggcgaat ttatttagaa
     3481 acaggtgaac tatgaacaag atggcgataa tcgatttggc gaagtcattt ttagcttcaa
     3541 aaataacagc aatggagttt tcagagcgga tttgtgttga gcggagaaaa ttgtatggtg
     3601 ttaagaattt gtctcaggat gtattgaatt gtggggagga gttgtttata gctgctgagc
     3661 gtttcgagcc tgattctgat agggctgatt atgaattcga tgataatgga cttaggattg
     3721 aagtccaatc tatcctggaa aaatttaaat tataaatatg agtgatttta atatctggca
     3781 tttaattata aatgtcagat actcactcat gacaaggaga tgttatgaaa aaaaagatag
     3841 gggtatcgat tattctcgtt cttgttgttt tgtgtctgac tgcttgtcaa aataactatg
     3901 taagggatgt tcagggagga acgattgctc catcatcatc ctcgaaacta accggggtag
     3961 cgattcagtg aaaaagatta aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4021 ctgcttgcaa acaaaaaaac caccgctacc aacggtggtt tgtttgccgg aacaagagct
     4081 accaactctt tttccgaagg taactggctt tagtggagca cagataccaa atgctgtcct
     4141 ttcagtgtag ccgcgataag gcttcaactt caagagttct gcgtatctaa atccactttt
     4201 cagttaccaa tggctgctgc cagtggcgta aagtcgtgtc ttaccgggtt ggactcaaga
     4261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4321 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
     4381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taatcggcag ggtcggaaca
     4441 ggagagcgca cgagggagct tccgggggga aacgcctggt atctttaagt cctgtcgggt
     4501 ttcgccacct ctgacttgag cgtcggtttt tgtgatgctc gtcagggggg cggagcctat
     4561 ggaaaaagcg ccaccggcgc ggccttttcc ccggctttgt cttgttgcac acatgttctt
     4621 tccggcgtta tcccctgatt ctgtggataa cagtattgcc gcttttgtgt gagcagacac
     4681 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagca
     4741 tcggcatctg catgttctcc ttacgcatct gtgcggcatt tcacaccgta catggtgcac
     4801 ttttcataca atcctcactg atgccacacg attaagccag tatacactcc gctatcgcta
     4861 cgtgactggg tcatggctgc gccccgacac ccgctaaaac ccgctgacgc gccctgacgg
     4921 gcttgtctgc tcccggcatc cgctcacagg caagctgtga ccgtctccgg gagctgcatg
     4981 tgtcagaggt tttcaccgtc atccccgaag cgtgcgaggc agctgcggta aagctcgtcg
     5041 gcgtggtcgt gaagggcttc gcagatgtcc gcctggtcgt cggcgtccag ttcattcagt
     5101 ttttccagca ggatcagcgt ctggcttctg ataaagcggg ccatgttaag ggctgttttt
     5161 tcctgtttag tcacagttct gttctccatg tgtggggagt ctgtcggagg tttttaatga
     5221 taccggtgaa acgcgaaagg atgctcacga tacgggtcac ggatgaggaa catgcgcggc
     5281 tgctggcacg ttgtgaggga aaacaacttg cgtcatggat gcgtaaggtc tgcctgggtg
     5341 caccgccatc gaaaacgtca ggtcttccga cactggcacc gccgctgttg cgccagttcg
     5401 cttcggtcgg caataacctg aatcagatag cccggaagat aaacagcgga cactggtcgg
     5461 ggcatgaccg ggttcatgtg gtggctgcac tgatggccat cggacgtgaa ctgagtgagc
     5521 tgagggatga ggtccgtaaa cagggagaac gggatgatag ttaagtttca tgccagggga
     5581 aaaggcggtg gcagtggtcc ggttgattac ctgctgggcc gggagcgtaa ccgggaaggg
     5641 gcgagaattc tgcggggtga tcccgaagag gtgcgggaac tcatcgatgc cacgccgttt
     5701 gcgaaaaaat acacctcggg ggttctgtct tttgcagagc agacactgcc accgggagag
     5761 cgtgaaaggg tgatggagag ctttgagcgg gtactgatgc ccggtcttga aaaaaatcag
     5821 tacagcatcc tgtgggtgga gcaccaggac aaggggcgtc ttgagctgaa ttttgtcatc
     5881 ccgaatatgg agctggtgag cggaaaacgg ctccagccct attacgaccg tgcggaccgt
     5941 ccgcgaatca atgcctggca gacactggtg aatcatcatt acgggctgca cgacccgaac
     6001 gcaccggaga acaggcggac acttgtcacc ccgaataatc tgccgaaagc gaaacaggag
     6061 gccgcagaag cgattacgag ggggctggag gctctttatc atgccggagc gctaaaaacg
     6121 cgtcaggatg tcactgaggc gcttacagcg gcaggttttg aggtggtgag gacaaccagg
     6181 agcagtatca gtatcgccga cccggagggc gggcgtaatc tcaggctgaa aggggcgctg
     6241 tatgagcaat cttttacaaa tggggacaga cttcgagaag aaactgaaag agcgtgccgt
     6301 ctctacagag aacatgctga acagcgagtt caggaagctc gaagcgtctg tagacaggga
     6361 actgaactta aacgggcaga aaatcagaga cgctatcagc gcacacacag cagcgctgaa
     6421 agaacaactg gagacgctga acagtgccgt gaacgcgcag ttcagcgcca cagagaacga
     6481 actggcccgg cggcaggaag agctgctctg gaagctgatc aaagggcgga tactgtaccc
     6541 gtcgctgacg gcgctttgcg tcacgggcgg gatttttctg gcgtcatggg ggctgattca
     6601 gtggcaggaa agccggattg cggcgaacat cctggctatc agggaccagg aagagacgct
     6661 ggcgaaactc cgggaaaaaa catggggtgt gacgtatcac gagggcagga acgggaaatt
     6721 tctggtattg ccgtccgggg tgaaggggga aaacaactgg acggtggaga agaagaacgc
     6781 agtaagactg gtcagggagt aagtgagtat gacagagctg gaaatgcact tactgaacgc
     6841 cttagagcaa ttgcagcagg actatacgca gcggctgaaa gactgggaga gcgccttcac
     6901 ggatttgcag catatgttcg agctgacacg acaggagaac gggcaactga gggaacagtg
     6961 cgacgacttg agtcagcagg tgcagagatt agccgggcag gtggaccgct tgagtcggtt
     7021 atttatcacg aacagcagat aagggatgaa caggaaaggc tggcacgcct ggaacgggag
     7081 cgtgcgcggt cctggcatga agagcaggaa cgggaagtac gggtatggcg tggcccgtca
     7141 atggggatgt gacggtgagt tgttccggga ggttgccaga tggtaccgga acgcgttaaa
     7201 aatgcatccg ggcgcgattc tggcgtgtcg tttttgttgc tgtgttttgc ctctttgcca
     7261 gatcgcgctc attcctcagc ctcatcccgc gatgtctgcg tatgtcatca actgaaaaga
     7321 tgg
//
LOCUS       DAZFYC010000187         7275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFYC010000187 DAZFYC010000000
VERSION     DAZFYC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.189"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            197..1132
                     /locus_tag="ACHKW5_004819"
     CDS             197..1132
                     /locus_tag="ACHKW5_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7514028.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1188..1730)
                     /locus_tag="ACHKW5_004820"
     CDS             complement(1188..1730)
                     /locus_tag="ACHKW5_004820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7514029.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1714..2025)
                     /locus_tag="ACHKW5_004821"
     CDS             complement(1714..2025)
                     /locus_tag="ACHKW5_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO7514030.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2210..3100)
                     /gene="cyoE"
                     /locus_tag="ACHKW5_004822"
     CDS             complement(2210..3100)
                     /gene="cyoE"
                     /locus_tag="ACHKW5_004822"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO7514031.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3112..3441)
                     /locus_tag="ACHKW5_004823"
     CDS             complement(3112..3441)
                     /locus_tag="ACHKW5_004823"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO7514032.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3441..4055)
                     /locus_tag="ACHKW5_004824"
     CDS             complement(3441..4055)
                     /locus_tag="ACHKW5_004824"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO7514033.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(4045..6036)
                     /gene="cyoB"
                     /locus_tag="ACHKW5_004825"
     CDS             complement(4045..6036)
                     /gene="cyoB"
                     /locus_tag="ACHKW5_004825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO7514034.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6058..7005)
                     /gene="cyoA"
                     /locus_tag="ACHKW5_004826"
     CDS             complement(6058..7005)
                     /gene="cyoA"
                     /locus_tag="ACHKW5_004826"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO7514035.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1987 a         1785 c         1798 g         1705 t
ORIGIN      
        1 ccatgtgcat tttttatctt acgaatatgc tagttttatc tcactaaata aacgatttgg
       61 tgacatggat gataatgttt caggctaaat gtgtgcttat tacttggacc tttatggcac
      121 tgtatttatt cattaaatgg atatttggtg gttaattatt atggaaatat atgacagtga
      181 ggacataaag gtatacatgt taactaaaag aatattattt tctgtaatgc tgatagtttc
      241 accaagcgtg gtggccagcg agaaagcgca tgaattgtat gacagcatat acggtggtaa
      301 acctgctccc gatgttataa atacactgca taaaatggct gaatcgggag atatcgacgc
      361 gcagagcctg ttaggctggg aatattatca gcctcgttat gataccaaac ctgatgttca
      421 ggaagcgatt aaatggttcg agttagcggc taaacaaggt gacagagaag ctccgttagc
      481 gctggggggt atctactacg acggtgagca ggtgcgggtg gattatgcca aagcgtatgc
      541 actatttaat caggccgcgc agcacggtgt gaatttagca tggtccaggt tgggcattat
      601 gtacgccaat ggtcagtatg ttgaggtaga ttgtaagaaa gcgaaggaat atcttgataa
      661 aggtgtccac atttatggtg gcccggaaga ctttctggct acttgtcgaa aagacatgat
      721 tgacagaaaa acggttgacg atacgttacc cgtgattacg gttacccgtt ctgggatgcg
      781 agataatttt ttagataaag gtttttcctg catggatagc ctcttcgcca ccaccaataa
      841 attaggcgaa gtggccaatc ttcgcgtgac attcagcatt cgtcgcccat ccggtaagga
      901 gattaaccaa acggttggct tcgcgccttt cggtttaaac cggctgaata ttagctttac
      961 tgattaccta tttggttcat ttaccagtaa ttcgtctctt attctttata aaccggaatt
     1021 tgagcgaaaa tcttgtgcaa ctgtaaggac cacaatcgtt gccgcaacgg caaccattaa
     1081 cggcaaggat gtggagttgc tgaaagcggg ggcaattgaa caaaagtggt aacgctgctc
     1141 agcccccggc tgaggcaaaa cttgccctgg cgggtggcga taataatcta agccgcgaga
     1201 atatgctgaa tcgccaaaaa cataggtaca gcaccaaaag catttggtgt cgctgaaagc
     1261 tgaacaaagc cgagacgagc ataaaaatta atggcggcag ggtcagcatc aagataaacc
     1321 cctttaattg gtaatgcctg gtgaattatt tttacatgtt caaaaaaatc acatagtagg
     1381 tcctgaccaa agccctgctt ttgatacttc cgtgctaccc ccaacatgac taatctgaca
     1441 acaccaattt ctgaaggttg tgaaccctga aggacgccag aaacgcgttg tttttccagc
     1501 gaatatgccg taaaagtaca gatgccgatc agttcaccac tttgtctgtc aataagtgct
     1561 ttagccgcac agtcgctatt acgcacactt ttctttagcg atgcgcgtac aaatttatcg
     1621 ataacagggt taccgcagtc aaactgtttt tgcccaggat aagtaatatc agactgataa
     1681 ttacaggaga ggactatggg cagagtaatc tcttcatgct tgtcgaccat cgctgttttt
     1741 ccttttcatt aacgccttga gggcgagcgt cggttgagca ggttcagcga tgagttggtt
     1801 catcagttcc cagctttgat tcgaaagctc acggcgacgt tggttatcaa gcacactttc
     1861 ggcgcgttcc atagcagcgc tcaaaataaa agcgcttaaa tctaccccaa cggcttgcgc
     1921 agcttcacga agtttgtttt tggcgtcggg actagttttg agttcgaccc gcgcattttt
     1981 cagcgtttta tcgacatcgg gtagagtggc tgtttttcca ggcattatga cctctttgat
     2041 taactgaatg aaatcgtagg gagtcgatct catacataat acggctatca accgtatgat
     2101 taatttatgt tcatctgctc actctgtcaa gacatacggt tattagccgt atctctgatc
     2161 tcagacaata aaaaaggtgc cgtagcacct ttttaataga gaggttttgt taccacacag
     2221 cagccagcag cgtatgcgag tccggtacca taaaatcaac ggacatcatc accgagaggg
     2281 cagtgatggc gatgatagag aagccgaaca gcttgcgcgc ccagattctg tcatcagcaa
     2341 ctttataacc gcgcagagcc atacctaacc accagacgct aaccgccgcg gcgaccacca
     2401 gatatttata tccagcgtaa ccgccaagag agagcatcag cgtggcaacg gcaaaggcga
     2461 tgatatacag cgtgatgtga ttcttcgcca ccgaaatgcc ttttaccact ggcaataccg
     2521 gaatgtttgc cgcctggtaa tccttaaagc ggaaaatggc gatggcatag gagtgaggca
     2581 tctgccacag gctgaagata gccagcagga tcgctgcgcc gctatcgaac tcgccggtta
     2641 ctgcacagta gccaatcacc ggcggcgcag cgccggagag cgaaccaatc aatgtgccgt
     2701 agactgagtg gcgtttcatg tacaggctat agacgccgac ataaaccaca aagcccatca
     2761 cccccagcca gcaggccagc ggattcgcgc caaaccacag cagcataaag ccagcaatac
     2821 ccagcaaggt ggcgtacacc agcgagacag caggagagat caggccttta accagcaccc
     2881 gattcttcgt cctttccatc tttctgtcga tatccctgtc gatgtagttg ttaaacacac
     2941 aacccgacgc cacaaccagt gacaccccaa ccagcgtgta gataaacagg ggataatcaa
     3001 tgctgccctt tgaggccagc aggaatcccc caatcaccga gatcaggttg ccaaagatga
     3061 tgcctggttt cgttacttgc aggtattgct taaacatcat aaccgccgct cttagtgcat
     3121 catcatgttg tagttgaggt tccacataat ccagatggag cctacaacca ggatagcgat
     3181 gattagcacg gtgaagacaa acgccgtcat gttccagcct tcatctgatt tggtattcat
     3241 gtgcaggaag cacaccagat gcaccagaac ctgtactact gccattgcca ggattgttcc
     3301 cagaattacg gccggagagg cagcccctgt catcaccatc cagaacggaa tcaccgtcag
     3361 aatgatcgac aggataaagc ctgtcatgta ggtttttacg ctgccatggg acgcgccgct
     3421 gtgatcggta gaatgactca ttacatcgcc cccatcagat aaacaacagt gaacacacag
     3481 atccaaacca catccaggaa gtgccagaac aggctcaggc acatgatgcg ggtacggtta
     3541 gtgctggtca ggccgcgacg agcgatttgt accatcagca ccgccatcca gataagaccg
     3601 gaagtgacgt gcagaccgtg cgtgccgacc agcgcaaaga acgctgacag gaagccgctg
     3661 cgatccggac ccatgccgtt aacaatcagg tgatggaatt cgtagatttc catcccgata
     3721 aatccggcac caaacaacca ggtcaacgcc agccaggaga taacctggct tttgttgttt
     3781 ttgtacatgg cgatagccgc catgccgtag gtgatggagc tgaacaacag caagaaagtt
     3841 tcaaccagaa cgaacggcag ttcgaaaatg tccttacctg tcgggccgcc tgcggtgccg
     3901 ttcaccagaa cggcataggt agcaaacaag attgagaaca gaatgcagtc gctcatcagg
     3961 tagatccaaa atccgaagat tttggttccg cctgcatcgt ggtgcccgtg ttcgtgcgcg
     4021 tgggcagtcg cgtgcgtcaa agtatcagtt gccatttttc agccctgcct tagtaatctc
     4081 atcgaaatgc tggttttcca gtttttcgat ttctgccacc ggcacgtagt aatccacgtc
     4141 ctcgtcgaag cttttcacga tccaggtgat gatcatgcct gcgaagccaa caatcgccag
     4201 ccaccagata tgccagatca tggcgaaacc gaagatggtg gagaaagctg caatgacgat
     4261 acctgcaccg ctgtttttcg gcatatgaat ttcttcatag tggtcaggct ttttatacgc
     4321 ttcgcctttc tctttcattt cccagaatgc atcacgctcg tgaacgtgcg gcacaacggc
     4381 aaagttatag aacggaggcg gggaagaggt tgcccactcc agcgtacggc caccccacgg
     4441 gtcgccagtc aggtcacggt tctggtcgcg gtcgcgaata gaaacgtaca tctgaataac
     4501 gaggcagaga atacccagcg caatcagtac tgcaccgcta gctgcaatca tcagcatggt
     4561 gtggaactgc gggtcaatct gctggctcaa acgacgggtc atgcccatga agcccagcgc
     4621 atacagcggc ataaaggcaa cgaagaagcc gatgatccag aaccagaacg cgcgtttacc
     4681 ccaggtttca ttcagtttga aaccgaaggc tttaggccac cagtaggtca tccctgcgaa
     4741 gcagccgaag accacgccgc cgatgatcac gttatggaag tgcgcaatca ggaacaggct
     4801 gttatgcaga acgaagtccg cgcccggtac tgccagcagc acgccagtca tcccgcccac
     4861 cgagaaagtg acgataaaac cgatggtcca cagcatcgca gaatggaaca cgatgcggcc
     4921 ctgatacatg gtgaacagcc agttgaagat cttcaccccg gtcgggatgg cgataatcat
     4981 tgtggtgata ccaaagaagg cgtttacgtt cgcgcccgca cccatcgtaa agaagtggtg
     5041 cagccaaacg atgaacgaca gcacggtgat acagacggtt gcccatacca gcgaggtata
     5101 accaaacaga cgtttacgcg agaaggttgc cgcaatttcg gagaacacac cgaaaacagg
     5161 caggatcagg atgtaaactt ccgggtggcc ccaggcccaa atcaggttga tgtacatcat
     5221 catgttgcca cccatatcgt tggtaaagaa atgggtgccc agatagcgat ccagggtcaa
     5281 caacgcgacg gtaaccgtca gaattgggaa ggaagcaata atcagtacgt tcgcgcacag
     5341 tgatgcccag gtaaatactg gcatcttgaa catggtcatg cccggtgcgc gcatcttcag
     5401 aatggtaacg aagaagttga taccggtaag cgtcgtacct ataccggata gctggagact
     5461 ccatatccag taatcgacac cgactcccgg actgtactct attcccgata gcggtggata
     5521 ggccagccag ccggtctgcg caaattcgcc cacgccgaga gaaacgttaa ccagaatcac
     5581 accaacaaca gtaaaccaga agcttaagtt gttgaggaac gggaacgcaa cgtcacgcgc
     5641 gccgatctgc agcggaacca ccaggttcat cagaccgata acgaaaggca tcgccacgaa
     5701 gaagatcata atcacgccgt gcgcggtgaa gatctgatcg tagtggtgag gtggcaggaa
     5761 gcccgcttcg cccgccgagg caagagcctg ctggctacgc atcataatgg cgtcagcaaa
     5821 accacgcagc aacatcacaa tcgccacgat gatatacatg ataccgaggc gtttatggtc
     5881 gacggaggtc agccactctt tccacaggta ggtccactta ccgaagtaag tgatcaggcc
     5941 aacgagcgcc agacctccca aaataatgcc agcgatcgta accatgacga taggttcatg
     6001 gaacgggact gcatcaagtg ataattttcc gaacatcttt attcttcctc aaccccttta
     6061 atgggcggat tccgcgtggc tcatgtccat gccttccata ccttcgtgtg cactgtgctc
     6121 accttctggc tgggtcatgt ccatgctctt accgtgagcc ataaacttgt taatcacatc
     6181 ggcaaacaag tctggtttca cgttggagaa atattccacc tggttgtatt cgctaggcgc
     6241 ggccagtttt tcgaacgcag ccatgtcaga catggtgttc ggcgactgtt tcgcttttgc
     6301 gacccattgg tcgaattcgg cgcgatccgg tgttgcaata gctttgaact tcatgcctga
     6361 gaagcccggc ccgctgtagc tggcggagat accgtcataa gtaccgggtt cgttggcgat
     6421 cagatgcagg cgagtctgca taccggccat ggcataaatc tggctaccca gacgcggaat
     6481 gaagaaggag ttcatcacgg agttggaggt cactttgaag tacaccggag tgttcgccgg
     6541 gaaagcgatt tcattcacgg tagcaatgcc ctgttccggg tagatgaaga accatttcca
     6601 gtccatggaa accacttcga tggtaatggg cttctcgtcg tgtgccagcg gcttgctagg
     6661 ctcaagagcg tgagtggttt tccaggtcag taccgcaagg aagatgatga ttaagatagg
     6721 taccgtccag accacagctt ccactttatt ggagtgtgac cagttcgggc tgtacttagc
     6781 atctttattg ctcgcacgat acttccaggc gaaaccaaca gccatcaaga ttgcgggaat
     6841 aacgacaatc aacatcaggc caaatgccgt cagtatcagt gaacgttgct ccagaccaat
     6901 ctgtcctttg ggatctaaca gcgcagaatt acagccactg agcaatacag tgcctgcaaa
     6961 taatgacaac catcccaaac ttttattgta tttcctgagt ctcatttaac gacctcaatt
     7021 ccacgggatc tggtggcgtt taaagtgtgt gggcatttta cgggaaggtt acattactgt
     7081 aaacatgatt aaattcctgt tacttggatt tggctgtcag gtcacatatg ttgcaaaaca
     7141 tatcaagttg attttaagaa gctgttgcaa aagggcgtta taacgaaagg ggagtgcaga
     7201 aggagtttgg acgggcaatt ggtataacca atctggaata aaacaattgt ttaacaaata
     7261 attatcatta tggtg
//
LOCUS       DAZFYC010000188         7259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFYC010000188 DAZFYC010000000
VERSION     DAZFYC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.190"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(156..518)
                     /locus_tag="ACHKW5_004827"
     CDS             complement(156..518)
                     /locus_tag="ACHKW5_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO7514036.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            803..1012
                     /gene="yibT"
                     /locus_tag="ACHKW5_004828"
     CDS             803..1012
                     /gene="yibT"
                     /locus_tag="ACHKW5_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO7514037.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1024..1611)
                     /gene="mtlR"
                     /locus_tag="ACHKW5_004829"
     CDS             complement(1024..1611)
                     /gene="mtlR"
                     /locus_tag="ACHKW5_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO7514038.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(1611..2759)
                     /gene="mtlD"
                     /locus_tag="ACHKW5_004830"
     CDS             complement(1611..2759)
                     /gene="mtlD"
                     /locus_tag="ACHKW5_004830"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7514039.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(2854..4767)
                     /gene="mtlA"
                     /locus_tag="ACHKW5_004831"
     CDS             complement(2854..4767)
                     /gene="mtlA"
                     /locus_tag="ACHKW5_004831"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO7514040.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5304..5666
                     /locus_tag="ACHKW5_004832"
     CDS             5304..5666
                     /locus_tag="ACHKW5_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7514041.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5669..6805
                     /locus_tag="ACHKW5_004833"
     CDS             5669..6805
                     /locus_tag="ACHKW5_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7514042.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAVALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1768 a         1820 c         1804 g         1867 t
ORIGIN      
        1 taaaaatgca ttttcagaat atcaaccagc ggtaaatcga agtaaaggga gtgaattgtt
       61 tggtaattag gcgagtagac aggagagtcc ccgcattgac cagctaaggc aagccagatg
      121 cgggttgata tgatcgaacc agtcgtctga ggcgcttaaa acgcagtctt agagaatcca
      181 gtcatctcct gcaggcccat ttcacggccc agcgcggtca ttgggtgcac aaccaccagt
      241 ccgcgaacac ttttcttcag cttgcccata tcagcctgct cttttttggt aattgcgcgc
      301 tgaaacggca tcttcatcag cttctgtgct tctttactca gtttttggct atgaacttca
      361 cgcaggcgag caatttctgc ttccagcgtg gctttctctt tttccagctc ggcatatttg
      421 tctgcggctt caaccagcga cagatcggcc tgctggtggc ggatggcgtc cagacgatcg
      481 ctgagacgtt tgatttcgtt tttttcgact tctttcatgt gatggcttct gataaagata
      541 aatttatagc ccctagtatg cgcgaccagg tgctttgttc acaactgatg acattgcaat
      601 agttctgtaa cgtaatcatt aacatgctta aaaactacgc aatttattga ttaattaaga
      661 aatctataga aaaaagataa taaacagcag cggatgatta ttgaggattc agcacgtttt
      721 ccttaatgtc atctttaccg ggaacctaaa ctaaaattgg cggccgcgca ttcccggcgc
      781 ggggtaatac ggagatatca tcatgggcaa attaggtgaa aacgttccgc ttcttatcga
      841 taaagccgta gatttcatgg catcaagcca ggcgttccgg gagtatctga aaaaacttcc
      901 tccccgtaac gcgattccgt ccggaatacc cgatgaaagc gtgccgttat atctacaacg
      961 tctggagtat tatcgtcggc tttatcggcc gaagcaggta gaggggcagt aagcagacgt
     1021 tgcttacttt tgaaaggctt tttttaagct gattctggaa atcagctcag tcagtgaaag
     1081 gaccattgtt gatcgcacag cctgttgata gcgctgaatt tgcattgcat acaaactgga
     1141 gtctgctggt tcaaactgtg gcggcggtgg taacgccgcc acgcagtgca gttcaccaaa
     1201 gggtccaagg atctcgtcgt cggtaaaggc gtactcgttg ccgtcgtgat ttagttcttc
     1261 acgcaatgcc atcagcagct ccgcatcttc gtattcctgg cggttaatga cgcccaaccc
     1321 gtaaatgagt tttaaacgca cagaaagatc gcccagcgga ccgtcgccgt cgagtaacgg
     1381 ttctacagca tacttcaccg cgtaatcgtc tttgcggaat acctgaagca ccagaagatt
     1441 taccgcctcg gtcaaaagct cgacggcggt gatcagaaag cttcgcacgg ttttgccagc
     1501 attcagacgc tcaagcacac ggttttcaaa ggcctgggtt tgttccattg ttgcctgcat
     1561 atctgacaat ctgttattcg ggcgcagggt gtcctgcgcc tggtccacca ttattgcatt
     1621 gctttataag cggttaccgc ctcggataca acctcgctgt tggcatcaag accggaaatc
     1681 tgtgccagcg ccgcctgcgg acctttgtca gcgatcagtg ctgccagttc ctgagcctgc
     1741 ggatcatctt cactgcggaa gtgcattgca ccagcaatcc cctgaatcag attcttgtgc
     1801 ggaaggctgt attccagcgt accgagcagt ggcttgatca gacggtcgcc agcactcagt
     1861 ttacgcagcg gctgacggcc tacgcgctct acatcatctt tcagatacgg gttctcaaaa
     1921 cgaccgagga ttttctggat gtacgccgca tgtttgtctg cgtcaaagcc gtagcgcttg
     1981 atcagtaccg caccactttc ttccatcgca ccttttacca ccgcgcggat tttctcgtcg
     2041 agaatagcgt cacgaatggt ctgatgacca gccagttttc cgaggtacgc ggttatagca
     2101 tgacccgtgt tcagggtgaa gagtttacgt tcgacaaatg ccatcaggtt gtcggttaac
     2161 tccatgcctg ggatgttcgg cagtgcgcct ttgaactgcg ttttatcgac aatccattcg
     2221 ctgaaagttt ctaccgtcac ttccagcgga tcgttagttg ccgaagccga aggcggtacg
     2281 atgcggtcaa cggcggaatc gacaaagcca acgtgttctt ctacccacgc tttggcgtct
     2341 tccggcaggg cgttcatcac atggcctttc agctgcgtgg taccgcgtac catgttttca
     2401 caggcgatga tgttcagcgg ggattcatta ccttgttctt tacgtttcac cagccctttg
     2461 gcgatagccg gagcaatacg ttccagcaca accgggccaa cggcggtagt gactaaatcg
     2521 acctgagcaa tcagatcaac gacatcatca ccaatgctgc tgacagcatc gacgccggaa
     2581 acggtatcta cctgctcggt ttcaccaacc acatgaacct gatagctatg acgggcattc
     2641 agggcatcaa gtaccacctg attgacatcg gcaaacgtca gttggatacc cgcgtctgcc
     2701 agcagtttac cgataaagcc acgaccgata ttacctgcgc caaaatgtaa tgctttcata
     2761 gtattaacct tcatcaatgt ttttacccga gagggctggg gtgaggcttt tccctcaccc
     2821 aaaccctctc cgtgaggaga gggtaggatt ggattactta cgacctgcca acagttccag
     2881 cacttcatcc acgctggtgg tgtgtgccag acgctcgatg acggactcat catccagtgc
     2941 attggtcagg ctggtgataa cctgaatgtg ctcgttgtta cgggctgcaa taccaatcac
     3001 cagacgggca atgtcatctt cttcttcacc gaagcgcacg ccttccgggt actggcagaa
     3061 caccacgccc gttttcagta cgcgatcttt cgcttcaacc gtaccgtgtg gcaccgcgat
     3121 agactcaccc agataagtcg gggtcagttt ttcacgatcc agcatcgcct gaacgtattc
     3181 cggctcaacg taaccgcctt tcaccagctg ctcgccagca aaacgaatcg cttcttcttt
     3241 ggttgctgct ttgcgaccga ggaagatgtt ctccgcgccc agtttgaaca ggttagcact
     3301 ggaatcgtca aagctgtctt tcagactgtc tttcactttc acttcgtttt cagtatggcg
     3361 ctgggcagca accagacgtt cggttaggct ggtgtacagg ccgctgtcga ggaagttggt
     3421 cagcgaaata tgctgcgcct gcggaacctg gcgcatagcg cgttcggtca ggtcacggtg
     3481 agtgatgacg aggtccacat ctggcggcag gttgttgatc gcgctgttag tgaccgaaat
     3541 ctgtgacaga cctgcatcct gaattttctt acgcagcacg cctgcgccca tcgcactgga
     3601 acccataccg gcgtcacagg caacgatgat tttacgtacg tggctcaggt cgttagtcac
     3661 atcgcccgca gacagcggag atgtgccttt agactcagct ttcatgtcct gcatacgacg
     3721 agttgctgct tcaatatcat cttcttcttt cactttgctg gttttcagca aaatagcaga
     3781 gacaacgaag gagacagcca tcgccgcaca cacacccgcg atgttagcga aataagcacc
     3841 ttttggtgtc atcgccagta cagcaaggat agaacccgga gatgccggag aaaccagacc
     3901 accgcccagg atagtcagcg tgaacacgcc agtcataccg ccgaggatga ctgcgaggat
     3961 cagacgcgga ttcatcagca catacgggaa gtagatttcg tggatacccc ccaggaagtg
     4021 gatgattgcc gcaccgcccg cagactgttt agcgctacca cgaccaaaga acatgtacgc
     4081 cagcagcacg cccatacctg gacctgggtt agcttcaatc aggaagaaga ttgatttacc
     4141 cagttcatgg gactgctgaa tacccagcgg cgagaagata ccgtggttaa tggcgttgtt
     4201 gaggaacagg attttcgccg gttcaacaaa gatagacgcc agcggcagca tgtcatggac
     4261 aaccatgaag ttaacgcccg cagccagcat tttggacagg gcttcaacaa tcgggccaat
     4321 gccgaggaat gccagaatag cgaggatcat cccgatgatg cctgcggaga agttattcac
     4381 cagcatctca aaaccggatt tgatcttacc gtctacccag cggtcgaagt gcttaatgca
     4441 ccagccgccc agcggacctg caatcataga accgaggaac atcggcatgt ctgcgccgac
     4501 gataacgccc atggtggtga tggcaccgac tacgccgcca cgttcgccgc ctaccagctt
     4561 accaccggta taaccgatca gcagcggcag gagataagtg atcatcggcc cgaccagctt
     4621 cgccagcgtc tcgttcggta accaccctgt tggaataaat aacgcggtga tgatacccca
     4681 cgcgataaac gcgccgatat ttggcatcac catgttgctg aggaaacgac caaagctttg
     4741 cactttgatc ttaatatcgg atgacataaa aacacccctt cttatgtttg ctgtcgcgca
     4801 ggctggaagc ccgaggtttt ttgttaatgt ggcggcagag gtagccggac cttgtagata
     4861 ctgcgaaatc tggcactgaa tcggttaact gtccagtcga cggccttttg tgtgatatca
     4921 atcacataaa tatagggggt agagaggtaa ttgacgtgac gttcatcaca aaacgcttgt
     4981 gtaaaaaagc aacaaggcgg cgattttcgc taaaaatggc atttaaatgt gagtagtgtc
     5041 acatttttgt tttgatggtt tgttgaatct ttgtgatctg aatcacaaga tattttctta
     5101 cggcgttcac ctgatgtgat ctacagcatg ttatgccttc gtcatacaat cgttatgtaa
     5161 ctaagcaact catcacctct aaaaattatt ctttggtgtt atggcgagaa tcgcgaaccc
     5221 ggattattgt caaaatatca gcctgactcc cttctggctg ccagcccaaa aacaacactg
     5281 ataaacgtga atccgaggca gatatgtttc taaactattt cgcgttggga gtgctgatct
     5341 tcgtctttct ggtgattttt tatggaatca tcgcgataca tgacatccct tatctgattg
     5401 ccaaaaagcg caaccatccc catgccgacg ctattcatac ggcgggctgg gtgagcctgt
     5461 ttactctgca tgttatctgg ccgtttctgt ggatctgggc gacgctctat caaccggagc
     5521 gtggctgggg tatgcagtca catgttgcgt cgcaggagaa agcgactgac ccggaaatcg
     5581 ccgcactttc tgaccgaatt tcccggctgg agcatcaact cgccgccgag aaaaagactg
     5641 actattccac gttcccggag atctaattat ggatctattg attgttttaa cttacgtggc
     5701 gctggcgtgg gcggtcttta aaatcttccg cattccggta aatcagtgga cgctggcgac
     5761 ggcggcgctg ggaggcgtgt ttctggtgag tggtttgatt ttgttgatga actacaacca
     5821 cccttacact tttaccgcgc aaaaggcagt gatagcgatc cctatcacgc cacaggtgac
     5881 gggaattgtt actgaagtca ctgacaagaa taatcagctt attcaaaagg gcgaggtgct
     5941 ttttaagctc gacccggttc gttaccaggc gcgagttgac agacttcagg ctgacctgat
     6001 gacggcgacg cataatataa agacgctgcg tgcgcagctc actgaagcgc aggccaacac
     6061 cacccaggtt tcagcggagc gcgaccgtct gtttaaaaat tatcaacgtt acttgaaagg
     6121 cagccaggcg gcggtgaatc cgttctcgga acgtgacatc gacgatgcgc ggcaaaattt
     6181 cctcgcgcag gatgcgctgg tgaaaggctc ggtggcggag caggcgcaga tccagagcca
     6241 gctcgacagt atggttaacg gcgagcaatc gcagattgtg agcttaagag cgcaacttac
     6301 tgaagcaaaa tataaccttg agcagactgt cattcgcgcg ccgagcaatg gctacgttac
     6361 tcaggtactg atccgcccgg gtacatacgc agttgccttg ccgctgcgtc cggtgatggt
     6421 cttcatcccc gagcaaaaac ggcaaattgt cgcccaattt cggcaaaact cgctgttacg
     6481 tctgaaaccc ggcgatgatg cggaagtggt gtttaacgcg ctacctgggc aggtgtttca
     6541 cggcaaactg actagtattt tacctgtcgt gccaggcggt tcttatcagg cgcagggggt
     6601 attgcaatca ttaacggtcg tgcccggcac ggacggtgtg ctgggaacca ttgaactgga
     6661 ccctaacgat gatatcgatg ccttacccga cggcatctac gcccaggtgg cggtttactc
     6721 cgaccatttc agccatgttt cggtgatgcg gaaagtgctg ctaagaatga ccagctggat
     6781 gcattatctt tatttggatc attgaggggg aggggggaga ttatggaact ttgctccttg
     6841 taaggactaa ttatttttgc atattacttc attagctgcg atgatttttc tttaatattc
     6901 attatgttaa taagttctgg atgagtgtgc tctcattgga ttttttatcc gtcttgttct
     6961 gccgcaggat gagactatac atcgtcgaga tcgcaatgat gatatgagtt gttcctcctg
     7021 cgacggctct aatcaaaaaa gagcaggagt gcttttccat ctattgcgat gatggcttta
     7081 ttatctgaag gatgacagtc acatttaaaa aatatttaat ctatagatca caatgtaggg
     7141 tcatggaata tatgtataca actattttct tcttcacttt taattatcgt attcattata
     7201 aaactaagtt aaaaaaactc ctggagagtg tgttattaat atatttttaa ataaaatta
//
LOCUS       DAZFYC010000189         7219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFYC010000189 DAZFYC010000000
VERSION     DAZFYC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.191"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..518
                     /locus_tag="ACHKW5_004834"
     CDS             93..518
                     /locus_tag="ACHKW5_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7514043.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            515..589
                     /locus_tag="ACHKW5_004835"
                     /pseudo
     CDS             515..589
                     /locus_tag="ACHKW5_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(818..1000)
                     /locus_tag="ACHKW5_004836"
     CDS             complement(818..1000)
                     /locus_tag="ACHKW5_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514044.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            1329..2201
                     /locus_tag="ACHKW5_004837"
     CDS             1329..2201
                     /locus_tag="ACHKW5_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7514045.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVAFVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2573..5422
                     /gene="cah"
                     /locus_tag="ACHKW5_004838"
     CDS             2573..5422
                     /gene="cah"
                     /locus_tag="ACHKW5_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO7514046.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGVARDTLIYAGGDQTVHGHALDTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGTNRLGHFSV
                     GNGMADNVVLENGGRLDVLEGHSAQNTLVDDGGTLAVSAGGKVTDVTMTSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASEQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            5533..>7219
                     /locus_tag="ACHKW5_004839"
     CDS             5533..>7219
                     /locus_tag="ACHKW5_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO7514047.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRR"
BASE COUNT         1839 a         1800 c         1989 g         1591 t
ORIGIN      
        1 gttgtcagta agcgtaaact gaccgccgta tgtagccatc agacgaaaat tggtaactta
       61 gacgcccatc tgatatagac ggacatctaa gtatggaatt acaggactgg cgaaaagaac
      121 ctcgtaaaaa ctattcgaat gaattcaaac ttcgtatggt ggaactggca tcacaaccag
      181 gagcctctgt tgcccgtatt gcccgggaac acgatatcaa tgataacctc ctgttcaaat
      241 ggctcaggct ctggcagaac gaagggcgta tatcacggcg tcttcaggta acgacctctt
      301 ctgacactgg cgttgaatta ttacctgtag aaataacgcc ggatgagcag aaagaaccgg
      361 tggcggccat tgcgccgtct ttatccactt ccactcagac cagtgtcagt gccggctcct
      421 gcaaggtgga gttccgtcac ggtaacatga cgctggaaaa tccatcgcca gagctgctca
      481 cgctgttgat ccgtgaactg accgggaggg gacgatgatc tcactcccgt caggtacccg
      541 catctggctc gttgccgacg ttaccgatat gcgtaaatcc ttcaacggat acgacatatg
      601 ctgaaggagg agcagtaatg gattaagtga taacaaatac ccagaaatag aggttcaaat
      661 ccagcttagt tagtacataa atactatttt gatgaaaatt attatatgtt tcctcagatt
      721 ttaatcaggc gcccagatta aaccaagtat tgagcgcctg attatgttta ttcattaata
      781 tctgtcattg attgtcttat tatcagctac ccccgtatta atcagagttt tcatttattt
      841 ttaatgcata atcagatgcc gcctcatcat tatcctcagt attatcatag ccgaaaactt
      901 ctcccggaca cgggcgttca cgataatagt catcgtcata gttttcatcg tcagataatg
      961 atatgtcgta ttctgaatat gattcaccac aattatacat gaaatcatct cctttggggg
     1021 gggcaataat tagtgagttt attcgaaaaa atgatgagcg ccagaccaat ccacccaact
     1081 tttgattata atcatgaaga tacttgtctc agtacaaatt gaatcaaact caatgcagca
     1141 tgattcacct ttatattctc tcagggaaac caaataccac tactcaacta ttaagaacaa
     1201 aattcgggat gtatgattat cagaagatac ctgcatccta aataatttca gacagatatt
     1261 atcgttgtga atcccccgcc acctttccgg tgcgcggggt tttgtctttt ttcaccggga
     1321 atacatgtat gaatccgtct gatgccattg aggcaattga aaaaccgctc tcctccctgc
     1381 cttactcgct ttcccgtcac atcctggaac atctgcgcaa actcaccagt cacgaacccg
     1441 tgattggcat tatgggtaaa agcggggccg gtaaatcctc actctgtaat gcactgtttc
     1501 agggggaggt caccccggtc agtgatgttc acgccggcac ccgggaagtg cggcgcttcc
     1561 gtctgagtgg ccatggtcac agcatggtta tcactgacct gcccggggtg ggcgagagcc
     1621 gggacaggga tgcagagtat gaagccctgt accgtgacat tctgcctgaa ctggacctgg
     1681 tactgtggct gattaaagcc gatgaccgtg ccctgtctgt ggatgagtat ttctggcgac
     1741 acatactgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacag
     1801 agccctgcca tgaatgggat atggcaggta ttcagccttc tcccgcacag gaacagaaca
     1861 ttcgcgaaaa aacagatgcg gtattccggc tgttccggcc cgtacatccg gttgttgccg
     1921 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
     1981 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgtgc acggaatctg
     2041 tccgggggca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggtgg
     2101 agagcgtctg tgttgccttt gttgcacgca cagttctgcg tgccgtccgt gacacggtgg
     2161 tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacagtctgc caggggaaat
     2221 gtctgattac attgcgttaa ccaactgaaa acatgataca aacacctcat gcccctcctg
     2281 tttctcttgc gtgactgctc tactgttaat agaataaaac gatcgataaa acaggtatcg
     2341 atcgtttata tcgatcgttt atagtggtta atggcctgcg aaaaggcagc tgaatctctt
     2401 catcatgcag aacggaattg cacacaacag actgatactt ctctgtctga tgaaggggga
     2461 gagcgtttac gcccgggacg tatcgctgtg cccgataact ctgttttgta cctgccggta
     2521 tccacttttg tgggtaccgg cttttttatt caccctctgt aaggaaaagc tgatgaaacg
     2581 acatctgaac accagctaca ggctggtatg gaatcacatt acgggcaccc tggtggtggc
     2641 ctccgaactg gcccgctcac ggggaaaacg cgccggtgtg gcggttgcgc tgtctcttgc
     2701 tgctgtcaca tcagtcccgg cactggctgc tgacaaggtt gtacaggcgg gagaaaccgt
     2761 gaacgatgga acactgacaa atcatgacaa ccagattgtc ttcggtacgg ccaacggaat
     2821 gaccatcagt accgggctgg aactggggcc ggacagtgaa gaaaacaccg gtgggcaatg
     2881 gatacagaat ggcgggatag ccggaaacac cactgtcacc acaaatggtc gtcaggtcgt
     2941 gctggagggg ggaacagcca gtgatacggt tattcgtgac ggcgggggac agagcctgaa
     3001 cggactggcg gtgaacacca cactgaataa cagaggcgag cagtgggtgc atgagggcgg
     3061 ggttgccacc ggtacaatta tcaaccgcga cggttaccag agcgttaaaa gtggcgggct
     3121 ggcaacagga accatcatca acaccggcgc agaaggcggc ccggattctg aaaatgtatc
     3181 cagcggtcag atggtcgggg gaacagctga atccaccacc atcaacaaaa atgggcggca
     3241 ggttatctgg tcttcagggg tggcccggga taccctcatt tacgcaggtg gtgaccagac
     3301 ggtacacggg catgcgctgg ataccacact gaatggcggt taccagtatg tgcacaagga
     3361 cggacttgcg ctgaacacgg taattaacga ggggggctgg caggttgtta aggcaggtgg
     3421 tgctgtcggt aacaccacca taaatcagaa cggtgaactg agggtacatg ccggcgggga
     3481 agccactgca gtcacccaga acacgggcgg tgcactggtt accagtactg ctgcaactgt
     3541 caccggcaca aaccgtctgg ggcatttctc cgtgggaaat ggcatggcag ataacgtcgt
     3601 tctggaaaat ggcggtcgcc tggatgtact ggaaggccat tcagcgcaga ataccctggt
     3661 ggatgacggc ggtaccctgg cagtgtctgc cggtggtaag gtaacagatg tcaccatgac
     3721 atccggtggt gccctgattg cagacagtgg tgccactgtt gaggggacca atgccagcgg
     3781 taagttcagt attgatggca catccggtca ggccagcggc ctgctgctgg aaaatggcgg
     3841 cagctttacg gttaatgccg ggggacaggc tggcaacacc actgtcggac atcgtggaac
     3901 actgacgctg gctgccgggg gaagtctgag tggcagaaca cagctcagta aaggcgccag
     3961 tatggtactg aatggtgatg tggtcagtac cggcgatatt gttaacgcag gggagattcg
     4021 ctttgataat cagacgacac cgaatgccgc gctgagccgt gctgttgcaa aaagtaactc
     4081 cccggtaacg ttccataaac tgaccaccac gaacctcacc ggccagggcg gcaccatcaa
     4141 tatgcgtgtt cgccttgatg gcagcaatgc ctctgagcag ctggtgatta atggtggtca
     4201 ggcaaccggc aaaacctggc ttgcgtttac aaatgtcgga aacagcaacc tcggggtggc
     4261 aaccaccgga cagggtatcc gggttgtgga tgcacagaat ggcgccacca cagaagaagg
     4321 tgcgtttgcc ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc tgaaccgtga
     4381 cagcgatgaa gactggtacc tgcgcagtga aaatgcttat cgtgctgaag tccccctgta
     4441 tacatccatg ttgacacagg caatggacta tgaccggatt ctggcaggct cccgcagcca
     4501 tcagaccggt gtaaacggtg aaaataacag cgtccgtctc agcattcagg gcggtcatct
     4561 cggtcacgat aacaacggcg gtattgcccg tggagccacg ccggaaagca gcggcagcta
     4621 tggcttcgtc cgtctggagg gtgacctgct cagaacagag gttgccggta tgtctctgac
     4681 gacaggggtg tatggtgctg caggccattc ttccgttgat gttaaggatg atgacggttc
     4741 ccgcgccggc acggtccggg atgatgccgg cagtctgggc ggatacctga atctggtaca
     4801 cacatcctcc ggcctgtggg ctgacattgt ggcccaggga acccgtcaca gcatgaaagc
     4861 gtcatcggac aataacgact tccgcgcccg gggctggggc tggctgggct cactggaaac
     4921 cggtctgccc ttcagtatca ctgacaatct gatgctggag ccacaactgc agtacacctg
     4981 gcagggactc tccctggatg acggccagga taacgccggt tatgtgaagt tcgggcatgg
     5041 cagtgcacaa catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg
     5101 tgaaggcacc tcatcccgtg acaccctgcg cgacagtgca aaacacagtg tgagtgaact
     5161 gccggtgaac tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggtgacat
     5221 gagcatgggg acagccgcag ccggcagtaa catgacgttc tcaccgtccc ggaatggcac
     5281 gtcactggac ctgcaggccg gactggaagc ccgtatccgg gaaaatatca ccctgggcgt
     5341 tcaggccggt tatgcccaca gcgtcagcgg cagcagcgct gaaggctata acggtcaggc
     5401 tacgctgaat atgactttct gattatacgg tatcccggaa atacgatttc aggaaaattc
     5461 atgcagtaat gggaaaggac gctcattgct gttcaccgca gttctgaatt attgaattta
     5521 taaaggtgaa tgatgcacga aaagaacatc gccctgcttt gtgatgaagc cgaccggctt
     5581 ttgcaactga atatcaatct gctccggcaa atggttgatg agccggatgt gttatctgac
     5641 agtaagaacg aaaacgggct gctttttgat aaacggaaag cgctgaaaag aattgaggag
     5701 ctggagggcg aacaaatcaa aaccgcccgc agggagatgg tgctggctgt ggtcggcacg
     5761 atgaaggcgg gcaaatcaac caccatcaac gccattgtgg ggcaggaaat tctgcccaac
     5821 cgtaaccgcc ccatgacctc ggtaccgaca ttgatccgcc acgttcccgg aaaaacggaa
     5881 ccggtcctcc atctggaaca tattcagccc gtccgcaatt tattaatcac actgcaggaa
     5941 aaactcgcca ccccggcagg gcagcaagtc gcacagtccc tgcagcaaac cggggatacc
     6001 cgcgaactac tggatattct ggcggatgat gtctggctta aaaatgaata ccacggggaa
     6061 gacgaaatct ttaccggact ggcatcgtta aatgacctgg ttcgtcttgc tgcggcaatg
     6121 gggactgaat ttccttttga cgaatacgca gaagtgcaga aactaccggt gatcgacgtg
     6181 gcgttcagcc acctggtggg aatggatgca tgtcagggaa cactcacact gctggatacc
     6241 cccggcccta atgaagccgg acaaccacag atggaaatga tgatgcggga tcaactacag
     6301 aaagcctctg cggtgctggc ggtgatggat tacacccaga tgaactcgaa agccgatgag
     6361 gaggttcgta aagagcttaa tgccattgct gacgtctcag ccggtcgtct gtttgtgctg
     6421 gtcaataaat tcgatgaaaa agatcgcaat ggtgatggtg cggatgccgt acgccagaag
     6481 gtgccagcaa tgctgaacag cgatgtgctg cccgcctccc gcgtttatcc cggctcatca
     6541 cgtcaggcgt acctggctaa ccgtgcgctt catgagttac gaaaaaacgg tgcgcttccg
     6601 gttgatgaag cctgggtcga tgattttatc agggaagctt tcggacctat ggtggaagag
     6661 gacgactgga aagacagcac taaggtaaat aaaaaggcag aaaaactctg gaatatatct
     6721 ctgatcgatc aactgataac ggaagtcata cagagctccc attccagagc ggcggcactg
     6781 gcggttgact ctgccgccgc aaaactgatg cagaatgcag aaaatgtcag cgaatatctg
     6841 tcattacgtc atcaggggct gcagcaaagc attcagtcac tacaggcgca tattaccagc
     6901 ctgctggcgg atatccagga gattgaggaa tgtcagaatc aggtgaccgg tgatgtcaga
     6961 atggccatgg aggacatcaa taccaaaacc ggggaattac tgacgaaggt ctgtgcctca
     7021 ctggaggagg agctgaacga ctatttcaga agcggtaaac gcaaagaaca gcaaatgctg
     7081 gaggaagaaa attcagcgca gccccgggag agaaacgcat tcgctttctt tcacgatatt
     7141 ttcggaaccg gaaatcagca tgacaggatg cgggactttg atccggacag ccccgaaata
     7201 aaattcagtg atcgcaggg
//
LOCUS       DAZFYC010000190         7122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFYC010000190 DAZFYC010000000
VERSION     DAZFYC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.192"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..715)
                     /gene="msrQ"
                     /locus_tag="ACHKW5_004840"
     CDS             complement(80..715)
                     /gene="msrQ"
                     /locus_tag="ACHKW5_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO7514048.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(716..1720)
                     /gene="msrP"
                     /locus_tag="ACHKW5_004841"
     CDS             complement(716..1720)
                     /gene="msrP"
                     /locus_tag="ACHKW5_004841"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO7514049.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1829..2242)
                     /gene="hiuH"
                     /locus_tag="ACHKW5_004842"
     CDS             complement(1829..2242)
                     /gene="hiuH"
                     /locus_tag="ACHKW5_004842"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO7514050.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2375..3046
                     /gene="hprR"
                     /locus_tag="ACHKW5_004843"
     CDS             2375..3046
                     /gene="hprR"
                     /locus_tag="ACHKW5_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO7514051.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3046..4404
                     /gene="hprS"
                     /locus_tag="ACHKW5_004844"
     CDS             3046..4404
                     /gene="hprS"
                     /locus_tag="ACHKW5_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO7514052.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEVDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4511..5362)
                     /gene="hchA"
                     /locus_tag="ACHKW5_004845"
     CDS             complement(4511..5362)
                     /gene="hchA"
                     /locus_tag="ACHKW5_004845"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO7514053.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(5955..7028)
                     /locus_tag="ACHKW5_004846"
     CDS             complement(5955..7028)
                     /locus_tag="ACHKW5_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7514054.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARNGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         1956 a         1521 c         1674 g         1971 t
ORIGIN      
        1 gatgtgatct gcgtaggccg gatagggcgc tcgcgccgca tccggcaggg ataacgcgta
       61 ctttgtcggc aatatgaagt tatacagata atttgttgtg aacctgcttg cgtaaccggt
      121 taaaaaggga acgcaacttc ttataccgta aggctaaaag cagtacagcc agcccagcgt
      181 agatgagcgg ttgcggtgaa ataatcttca ctgaccacag ataatgtatt ggggccagga
      241 tcgcgacaag atagacgaaa ttatgcaact gttgccagtg cttgccgagt tttcgctgca
      301 tcgcctgggt tgaagtaaac gctaaagcaa gcaaaataac ccagctgata attcctaacg
      361 ttaaataagg ccgggtaatt aactccttgc ctaataacgc cagattattc acgcccagct
      421 ccagcaatgc gtaactggtt aaatgcagtg tcgcccaggc aaagcaccat aagcctaaca
      481 ggcggcgagt gcgtatcaat aacggctgtt ttgcgtagcg tgccagaggt gtgattaaca
      541 aggtcgccag caaaaatttc agtgcagtgc gaccggtaaa atgctgaata tctttcaccg
      601 gatcggcacc cagtccaccg tgattgatcg cccagaccag ccagagaaac ggcaacaatc
      661 cggcaagatg caggctaact ttcagccatg tcacctgttt tgctgtcaga cgcattcaga
      721 aattctcccg caaatccagg ccacgataca gcgatgccac ctggtcggcg taaccattaa
      781 acagtagcgt tggctggcgc tgaacatcga ggatgccgcc tgaaccaata aatcgttcgg
      841 tagcctgtga ccagcgcggg tgatcaacat gcggattaac gttggcgtaa aaaccgtatt
      901 cgtcaggcgc tgccagattc caggtggttg gcggacgctc gcgggtcagc ttaatactga
      961 ctatcgattt aatcccttta aagccatatt tccacggtac aatcagtcgt accggcgcgc
     1021 cattttgtgg cggtaacgcc ttgccataaa cacccacggt catcagtgtg agcggatgca
     1081 ttgcttcgtc gagacgcaat ccttcgacat aaggatattt cagcccgccg ccgataaagc
     1141 ggtcctgctg gcctggcatt tgttccggtg cataaattgt ttcgaaagcg acatacttcg
     1201 cattgctggt gggttcggca agcgccagca atttgtgcag cggaaaacca atccacggca
     1261 ccaccatcga ccatgcctcc acgcagcgca tacgataaat acgctcttcc agcgggaagc
     1321 gacgggttaa atcatcgtga tcgagggtca atggttttgc cacttcgccg ctgattttca
     1381 gtgtccatgg atcggttttc aggctaccag cattagcggc gggatcggct ttatccagcc
     1441 cgaattcata gaagttgtta taaccggaga ctttatctac tggcgtcagt ggcaggttat
     1501 tttgccaggc ggcaggcttg ctgaactcca gcggttttcc ggcgggggcg ggcgggcgat
     1561 cgttcccttt aaaccagcta agcagatcgg catgcgcagc gtgaggcaaa gaaagtgcag
     1621 ctgcgctgat gcccagtgct tttaacacct gtcgacgctt cataaagaat accgactcgg
     1681 ccgtaacatc tgattctttt aaaaattgat tctttttcat aagccatcac acttttttgt
     1741 cgttagccag caagagccgt ttactgttaa tctgtcaggg agtgccctcc ctaacagtag
     1801 cagagatctg cttttacata tttatgcatt aactgccacg ataggttgaa tacccatatt
     1861 ggctaagtaa taaaggcaca tgataatgct cgttcacttt attaatatga aattcaacgg
     1921 ggatctcagg gaagaaactt tcaagatttt gtttcttgaa atagtccccg gttttaaata
     1981 cgacacggta atcgccagta gtcgcagttt gctcgggcca cagtgcctta attcgtccat
     2041 ccttatctgt tttggcggta ttaagttgta accagccgtt gtccgctttc ttttcaagag
     2101 tgactgtcac gtcggcagcg ggttttccgg tttgctggtt caaaatgtgc acgctaagaa
     2161 tgttttgttg tgcggcataa accaaagaag gtaatgaaaa tgctgccgtt actacggaga
     2221 gtactaaata acgctttaac atgtttatat ccttgtcatg tgaatgagtt ttgaggatag
     2281 cgaaaaagcg gccagggtaa ccttacagca gcattacaat tttgtaatga accaggctgt
     2341 ttctataaca tatgatttat ggcatattat tttcatgaag attttactta ttgaagataa
     2401 tcaaaggacc caggagtggg taacgcaggg gctttccgaa gcgggttatg tcattgatgc
     2461 cgtttctgat ggcagagatg ggctttatct tgcgctgaag gatgattatg cattgatcat
     2521 tctggatatt atgcttccgg gtatggatgg ctggcagatc ttacaaacgt taagaacagc
     2581 aaagcaaacc cctgttattt gccttactgc aagggattct gtcgatgaca gagtcagagg
     2641 gctggacagt ggagcaaatg attatctggt aaaacctttt tcattttctg agttgctggc
     2701 aagggttcgg gcacaattaa ggcaacatca cgctttgaat tcaacattag aaatcagtgg
     2761 cttaagaatg gactctgtaa gtcaaagtgt gagcagggac aatatcagta ttacactgac
     2821 gcgcaaggag tttcagttac tttggctact ggcctccaga gctggcgaaa ttatacccag
     2881 aacggttatt gcgagtgaaa tttggggaat caactttgat agtgatacca atacggtgga
     2941 tgtcgccatt cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa agctaatcgc
     3001 cacaatccgg gggatgggct attcattcgt agcggtaaaa aaataatgaa aagattatct
     3061 ataaccgtcc gtttaacctt gctttttata ttgctgctgt ctgttgctgg cgctggaatt
     3121 gtctggactc tctataatgg cctggcaagt gagttgaaat ggcgcgatga tacaacactc
     3181 attaaccgga cagcacagat caagcagttg ttaattgatg gggtaaatcc agatacgtta
     3241 cctgtatact ttaaccggat gatggatgtt agtcaggata tcttgatcat tcatggtgat
     3301 agcatcaata aaattgttaa ccggacaaat gtcagtgatg acatgttaaa taacatacct
     3361 gctagtgaga caatcagcgc agctggcatt tacagaagca ttattaatga tactgaggtt
     3421 gatgctttac gaattaatat tgatgaagtt tcgccatcat taacggttac tgtggctaaa
     3481 ttggcttcag ccagacataa catgcttgaa cagtataaaa ttaatagcat tataatttgc
     3541 attgtcgcca ttgtactttg ctcagtatta agtccgctgt taatcagaac gggattacga
     3601 gagatcaaaa agttgagtgg tgtaacggaa gcgctgaatt ataacgatag ccgggagcct
     3661 gttgaggtta gcgcattacc gagagaacta aaacctcttg ggcaggcgtt gaataaaatg
     3721 catcaagcct tagtcaaaga ttttgaacgc ctaagtcaat ttgctgacga tctcgctcat
     3781 gaacttagaa cgcccattaa tgcattactg ggtcagaatc aggttacgct cagtcaaacc
     3841 agaagtatcg ctgaatatca aaaaacaatt gccggtaaca ttgaagagct ggaaaatatt
     3901 tcgcggttaa cagagaacat actgtttctt gcccgggcag ataaaaacaa tgttttggtg
     3961 aaactggact cgctttctct caataaggaa gtcgaaaatt tgttggatta tcttgaatac
     4021 ctttcagacg agaaagagat ttgctttaag gtcgagtgca atcagcaaat ctttgcggat
     4081 aaaattttac tgcaacgaat gttatcgaat cttattgtta atgccattag atattcacca
     4141 gaaaaatcgc atattcatat aaccagtttt cttgatacca acggctatct taatattgat
     4201 gtcgccagtc ctggaacgaa aattcatgag cctgaaaaac tcttccgtag attttggcgg
     4261 ggagataatt cgcgtcattc cgtaggtcag ggactaggcc tttctttagt caaagcgatt
     4321 gccgaattac atgggggaag tgctacgtat cactatctca ataagcataa tgtgttccgg
     4381 attacgttac cgcaaagaaa ttaacataag gcttcagtac gccataaatt aaccgtgaaa
     4441 aaacgcagcc acgtcgtgag tactaacgcg gctgcgattg attagcgctt acattcaaac
     4501 gtaacaggga ttaacccgcg taagctgcca gcatttcctg cgccgccagt ttacccaacg
     4561 cattcgctgc aaaaggactg tcgccagtga gaactttacg gtccttatgt actcgcccgg
     4621 tgatgtcgtc attaataatg ttcatgccca ttttcttcag ctcttcgccg aagtaccagg
     4681 tgagatgacc cggcatatag cctatctctg gcgtttgttt gtctgcggcg tctgggaatg
     4741 cgcaaatgga ataaccattc agtgggttat cgccgtggcg aagcgccaga aaagccgccg
     4801 ggccgtggca aagggagata acaaaacggt catttttgat tgcccactgc aaagcagcag
     4861 ctacgtcctg gctttctggt aaaccaataa gtgcgccatg accaccagga acaaagattg
     4921 ctgcatattc gctatcagcg ttgaggctgg cgacaacatc cgcgagtttc ttcggattgc
     4981 ggaacaacga tttatgttgc tcaaagaatg gcattacttt ttcatctttg tgcggcatag
     5041 cccagtattc aaacttggtc atcagaccgg aaatggtcgc cacttcgaat tcgaaacctg
     5101 cagcatggag atgatacaac ggcagcaacg tttcaatcgg atggttaccg gtcgagaaca
     5161 gttttccgtt atcggttggc aaataacgtt cgtccgcggc gatcaccaga attttatgct
     5221 taccgcgata cggttttgga tagtccacgc catcaagatc agagacggga ctggtatatt
     5281 ggctaagcga atattctgaa gggaagaatg cattatcttc agcaatatcg acctgcggat
     5341 ttttacttgt ttgaacagtc atagtgtatt ccttattgtt gcttagttag ggtagtcact
     5401 atatttgcga ctgagctggt cgctattttt aaccagtctg gaaaattgag tcaatatatc
     5461 gaaagcggta gtcggcgggt gtaagtatgt aatgatgatg ctgcactggt gttgaatatt
     5521 attcacgcca aattttcctt attgagcaac gtcgtcgcaa gtggcctcaa tgattttaga
     5581 ccattatcaa aggtacgggg cggcggggag agatttagtg ctgtgaaata ttgtggtact
     5641 gatgaatatt tatatcgcgg attggtagag aaaagattat ttcatgcact atttttgcaa
     5701 atatttctat atgggaagcg tgtttgttac ggggtaattc atcagaatgt gcatttgggg
     5761 tgttggctgg aagagtcgct ggtagatgtt attgtggcag gaggggcagg gtaaggtgct
     5821 gtatcgatat gctttggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa
     5881 agcctgagtt cgatgaggga cgaactcagg ctagtgttat gttttacagt aacacgccgc
     5941 ggcgcgttgg ctgattagaa ctggtagacg ataccaactg caaactggtc atcagttgcg
     6001 gcatcggtaa tcgctgcatc gtctttatcc agcaggttaa atttgtatgc tgtgtagacg
     6061 ttcatgttct tattgaagta gtaccaggta ccaacttcga tataatttac gcggtcagca
     6121 tcaacacctt tatgtccatt acgagcataa agatctttgc ctttggaata tacgtagcct
     6181 aaagacggac gtaaaccgaa gtcgaactga tactgtacaa cagcttcaaa gttttgggtt
     6241 ttatttgcaa aatgatcatt agccaaaacg gtcatgttgc gggtttcacc gtacatcatt
     6301 gctgcataga tattatttgc atcatacttg gctgctaatg accacacttc agcgttatca
     6361 ccgtagccgt caccagcttg ttcattagta cgatcagatt tgctatatgc acctacaaaa
     6421 ccaaaacctt cgatgttgta gtctacagac aacgcatagc catcaccgtt ttgtttagta
     6481 acgccacgat tgctgttctt accttgatat tgcacagcaa aattcagacc atcaaccaga
     6541 ccaaagaaat cagagttacg gtaggttgca acaccgttgg tacgaccgtt catgaagttg
     6601 tcagcggaag cccaggaatc accaccccat tcaactaaca tatcggtata agctgcggca
     6661 tcataagcaa caccgtagtt acgaccatag tcgaaagatc ctaactcgtt atatttcaaa
     6721 cctgcgaagg ttaaacgggt tttttcctga ttagagcctt cgggcttgct ggcgtcgagg
     6781 ttgtattcaa actgaccaaa accagtcagt tcgctggtga tttgggtttc acctttaaca
     6841 ccgaaacgag cataggaggt atcttcgttt tcgctgttat tatcatcagt atttgaccag
     6901 atgcgttcgc caaccatctt gccgtagaaa tcaactttat tgccatcttt attgtaaatt
     6961 tcagccgcat ttgctgcgcc agcaactaat aacgccggga ccagcattgc cagaactttt
     7021 cttttcatta tgtattccct tgtgattata atcttcatga atatatcaat aagtgccgtt
     7081 atccaaaaaa aagcacattt ggatactatt ctatgaagtt ca
//
LOCUS       DAZFYC010000191         6968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFYC010000191 DAZFYC010000000
VERSION     DAZFYC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.193"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(359..844)
                     /gene="sixA"
                     /locus_tag="ACHKW5_004847"
     CDS             complement(359..844)
                     /gene="sixA"
                     /locus_tag="ACHKW5_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO7514055.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1047..3191)
                     /gene="fadJ"
                     /locus_tag="ACHKW5_004848"
     CDS             complement(1047..3191)
                     /gene="fadJ"
                     /locus_tag="ACHKW5_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO7514056.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3191..4501)
                     /gene="fadI"
                     /locus_tag="ACHKW5_004849"
     CDS             complement(3191..4501)
                     /gene="fadI"
                     /locus_tag="ACHKW5_004849"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO7514057.1"
                     /translation="MGQVLPLVIRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4681..4965)
                     /locus_tag="ACHKW5_004850"
     CDS             complement(4681..4965)
                     /locus_tag="ACHKW5_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO7514058.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5337..6677
                     /gene="fadL"
                     /locus_tag="ACHKW5_004851"
     CDS             5337..6677
                     /gene="fadL"
                     /locus_tag="ACHKW5_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO7514059.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1756 a         1865 c         1633 g         1714 t
ORIGIN      
        1 caaacaatga acatataatg cgattggcat ttatccactt ttgacaatat aatatataaa
       61 cagtatagcc agaatttaat caagtttcaa ttacatcctg cctgattatt caactctatt
      121 ttctctatta tctgtattaa taaatgacaa tgagtgtcaa acagacatta ttcatttcaa
      181 catgtaactt tgggcaaaat caagatacgc gcagaagaat aatttccgaa tgaatgtaga
      241 aagtgtttta tttatagata aggagagtct tcgatagtag tgaggttgac agatatccgc
      301 tgcctgatga gcaattatgc ccaatgaatc tacctcattg ggcataattt ggaacacatc
      361 agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat gtaccgtttc
      421 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
      481 ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc accagtaccg
      541 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
      601 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tcccctactt
      661 cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
      721 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta gtggtcagag
      781 gacgaacgga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
      841 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
      901 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
      961 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc cgccattcag
     1021 cgcggattca tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa
     1081 ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac
     1141 tgcgtggcaa gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
     1201 cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg
     1261 tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt cagcatcaac
     1321 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
     1381 agcgggtaaa tggcgggatc gacctgtttt ttgcttttac gccctttctg accataaaga
     1441 tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
     1501 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
     1561 ccggtgtcga ttcctacctc atccaaaagt tggattggac ctaccggaaa accaaatttc
     1621 actagcgcgg catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg gatagcttca
     1681 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
     1741 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg cgctgatgta
     1801 cccgcatgag gaataatctc caccagcggc attttttcca ccggactgaa gaaatgcagg
     1861 ccgataactt gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg taaagatgac
     1921 gtattcgaag caaagatggt atgagcggcg ctattttgct caacttccgc caccatctgt
     1981 tgtttcaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
     2041 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
     2101 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
     2161 ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc agtgacataa
     2221 gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa tggcgcaggc
     2281 ggtgcatcac taccgggatc tttcttcacg tccgtactgg caaaaaagat actacgcagc
     2341 gcctgcgatt gtggcgtcat cgccagttcg ccaaacgccc gagcttcggc gtcataaccg
     2401 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
     2461 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
     2521 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
     2581 ttcttcgcca gctcaacagc gacttccagc aaaatggagt gcggaacaac gtcatccacc
     2641 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat
     2701 gctgtgctga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
     2761 ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
     2821 tggcacgcca gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata
     2881 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagtgct
     2941 tccgcttctt gcgccgtttt gcagttgccg atcatgttga tgtctgcgcc agcaatgaag
     3001 ttgtccggtt tagcggagac aaacaccacg cctcgcaact ctttgttttc acggagttgc
     3061 ttaataatgg cgcgcacctg cgaggcaaac tccgccttca gggtattcat tttctcaccc
     3121 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacactgat
     3181 gccatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
     3241 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
     3301 tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa
     3361 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca cgagcaaaac
     3421 gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat
     3481 cgatcaatgt cagatcgctc attgtcaaac cggcacgttc cagcgccagc ggtgttgacc
     3541 aggccggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg tagctgcgca
     3601 gataccccag cggcaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
     3661 ccgctgcgcc atcggtcagc ggcgtactgt tcgccgccgt taccgttccg tgtttgcgat
     3721 caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga atattgttgt
     3781 cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
     3841 taccgtctga ccacgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
     3901 gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc atccgcaagc
     3961 cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa cgcaggcgag
     4021 agaagagttt cagtcgctgg ctcatggttc gagctttgtt gacatcaacc agcacgcgcg
     4081 ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca ccggcaatcc
     4141 ccgctcggat agttcccgcc atcaggcttt ctgcgacatt tgcaaccgcc tggaaactgg
     4201 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg gtaccgagaa
     4261 caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca aagaccagct
     4321 gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
     4381 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
     4441 aaccgctaac aatggcgata cgatcgccct ggcggataac cagcggtaaa acctgaccca
     4501 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt
     4561 tttttacatt tagccaagcg gagaaaaaag aaagtgggag ctatgacaca gtgaagacag
     4621 gagaaagaaa agaaaacgcc cctgccagca actgacaggg gcgaacgtac ccgagaggaa
     4681 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
     4741 atccaggcgc acaccgtcag actcttcagt gaaagtcggg gtgattttgc acggttcgga
     4801 ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggct
     4861 tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg tgccaacatc
     4921 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
     4981 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
     5041 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
     5101 aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca gctggatcgc
     5161 gtttcttaga tcatatttga aaaaagatgg aaacatactt gcaacattcc agctggtccg
     5221 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
     5281 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
     5341 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
     5401 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
     5461 attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac cccgcattga
     5521 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtga
     5581 atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
     5641 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
     5701 ctattacctc taactatggt ctggcaacag agtttaatga tacttatgca ggcggctctg
     5761 tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
     5821 ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac
     5881 gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct cctgccgggc
     5941 aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
     6001 tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc ctgtatgaac
     6061 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
     6121 gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca attcctaccg
     6181 cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
     6241 tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg gcttacacca
     6301 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
     6361 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
     6421 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gctcagaatc
     6481 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcat
     6541 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
     6601 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
     6661 ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgcata gcaggtgact
     6721 ttaactcccc cacttcaccg aaagtagtgt cctcattgct tacctcctga gttttgcaaa
     6781 cagcctgttg gcagcttgcc ctttcaaatc agtaagtggt attcacagac aaacctgaag
     6841 ggaaatgcat attttcaagc gttctgctcg tccttctcaa agagtttact tttctgaatt
     6901 tccaggatac tccccccccc ctggctattg tgcgctcata cactcaaatt aaagataggt
     6961 tctaaata
//
LOCUS       DAZFYC010000192         6812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFYC010000192 DAZFYC010000000
VERSION     DAZFYC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.194"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            187..1584
                     /locus_tag="ACHKW5_004852"
     CDS             187..1584
                     /locus_tag="ACHKW5_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514060.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            complement(1581..1781)
                     /locus_tag="ACHKW5_004853"
     CDS             complement(1581..1781)
                     /locus_tag="ACHKW5_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514061.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(1778..3169)
                     /locus_tag="ACHKW5_004854"
     CDS             complement(1778..3169)
                     /locus_tag="ACHKW5_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFO7514062.1"
                     /translation="MITFKPRPYQDLIINHQIETLRGNIWAGMGMGKTVATLTTLEDL
                     FMAGAETRPALVLAPLRVAASTWPDEALKWKHLRNIEVQPIVGNVKARSTALANSNAS
                     VFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVRR
                     WINLTGTPAPNGLVDLWGQSWFVDLGQRLGRTYGAFTSRWFKSIQFPGQSWTKLEPFA
                     HSQGEIQRALADVTLSLDATDWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELNG
                     EGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVAY
                     HWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNILV
                     FFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIAADTMDEMVMERRNSKRTVQ
                     DILLDAMKKRGIA"
     gene            3208..3795
                     /locus_tag="ACHKW5_004855"
     CDS             3208..3795
                     /locus_tag="ACHKW5_004855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514063.1"
                     /translation="MNVKDLSITTVLLAALYFMAFSFYKGYSSFYGFPVSFISIGIEE
                     IVKFSVIALGLLCTLVALLHIDTEEKNIPWGVYLFFFILASLLSYWTMYLYGGSSYLY
                     EKSIRDVATQAILLGFISPISVRAVSVFIRNEFRIKNKTHGVMFFISIAMLPSVLGWA
                     WAYLSDEPLFYSKKYDAYIIESYNGKFVLGNCKKK"
     gene            complement(3925..4194)
                     /locus_tag="ACHKW5_004856"
     CDS             complement(3925..4194)
                     /locus_tag="ACHKW5_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO7514064.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGHRSVPDRIVLLPG
                     GRIVFVECKSPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            complement(4184..4483)
                     /locus_tag="ACHKW5_004857"
     CDS             complement(4184..4483)
                     /locus_tag="ACHKW5_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO7514065.1"
                     /translation="MIVYVAGPMSGYEQFNRPAFHSAAKRLTDKGYVVLNPATLPDGL
                     TQAQYMDICLAMLRCADTIYMLKEWEYSAGARAENALAEKLELNVLFEEWDSDGL"
     gene            complement(4480..4695)
                     /locus_tag="ACHKW5_004858"
     CDS             complement(4480..4695)
                     /locus_tag="ACHKW5_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7514066.1"
                     /translation="MSFKYRDNPLYYRAAREALRLEQSGEYDRAAKVWAKANRESHHE
                     LNQEWSERRSDFCLMQNMREKRKVVEE"
     gene            complement(4701..6764)
                     /locus_tag="ACHKW5_004859"
     CDS             complement(4701..6764)
                     /locus_tag="ACHKW5_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFO7514067.1"
                     /translation="MSNILWGDLETYCEIPITNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIAAPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDVELARAAIDAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEEWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMRQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYDESVKRKSTYGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEKGNITYMGVNSYSRKWQRLKTYGGKLVENVTQAAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITEAPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
BASE COUNT         1584 a         1708 c         1703 g         1817 t
ORIGIN      
        1 aaatcagtaa gttgtatcga gtttgtatgc tttactgtca ttaacttact gtatgttgat
       61 ccagtgtatt taaccttgat aaactcagtc cagcaacaca aaaccgcaac acattgcatt
      121 ttgtcccgta gaaaagactt gtatgtgtga gcttgttttc tgcgcctacg cagataagga
      181 ttgagaatgc cgcgcactgt aacacataat ccggatagcc ccaataatga cgatgtttta
      241 gccgcatctg aaaaatggga cgcctgtaaa cccccctata ccagcgcaca catgaaaatc
      301 tgtgttgctg ccgccaaaat catcctcgct gcttccggcg tggctcgccg ttccaaatac
      361 gaaaaagaga actatctccg tatcgatttc agcaaagctg gtaaggttac attttacgcc
      421 gagtttccaa aaaagatggg cctcaagggt aaaaagctcg gcgagtggcc ggagctcgct
      481 atccagctgg cgcgcgaaaa agcgctaggt atggctgacg gtggcctgcg ggcagagtcc
      541 gtacatgcag cgctggaaat gtaccgggat gacctcaaag ccaaagtcgc ccggcagaag
      601 ctgagcccgg acagtttcac aacctacggg gtgcgtatcg accggattaa agccacgttc
      661 ggcgagcgcg aggtgttcag cgacgtaaca tacaatcggc tggtggaagt gctggacgag
      721 tggatcgcca ctcgctcgaa caataacgcc ctggagttgt ttgccgagct ccgtcggttc
      781 tggaagttct gcgcacctac tctttgcaat ggccgcaatg ttgccgccag tctgccagat
      841 gattatgttt cctcccgcgt acagaaaccc acccccacac ggctttttac cgatattgaa
      901 tcaatcgccc gactctggct caatgttgct gcctgcacct ctgtacacca gaagaatgct
      961 gttcgcttca tgatcatcac tggtgttcgt ccgattaatg tccataacct gcgctgggat
     1021 tacgttcacg aggaggctgg tgaaattgtt tatccggaag gggttatcgg catgcgaggg
     1081 gctatgaaaa cacaaaaggc tttccgcctg ccgataacgc ctgagatccg gcggattatc
     1141 gacgagcaga aagcctggcg tgattcagtt cctgagtgca acagggatta tgtatttttg
     1201 cagccacgtg atccaatgca gccattttca aaacgatcac tggataagct ggtgaaaaca
     1261 tacagcccgg acggggctgt aaaaggaata aaacatgatg ggactgttaa agggaaagac
     1321 ggtgcattta atacgatgtg ccgtaaattc cttaagagca atgttattgc cttgatgaag
     1381 gaaagaggct attcccgctc agaccgaagg gaaatcagcc tcctttgcct tcaccactcc
     1441 agcaagtcag atgacccgat ggcagaacat tacgactttt ctgatgagat tttacaggaa
     1501 gagattgcgt tgaagcgcga agctttcgag gctcacgagc ggagcatact tgcgcaggtg
     1561 gcattgctac ggcggcgagg ttaatactgg ctgcgacatt tttgaataaa agcgtcgaca
     1621 tttcggcgct cataacgaac tacttttgca ctgaaacgaa ttggtgccag gatagcccga
     1681 tgacgatgct taatattcca ctcacatagc gttttctgtg taatacctaa cttttggcat
     1741 acttcatccg gggtgagtaa atcgtcgggt ttctcgctca tgctatacct ctctttttca
     1801 tggcatcgag caggatgtcc tgcactgttc gttttgagtt gcgccgctcc atcaccattt
     1861 cgtccatagt gtcggcagca ataatgtggt gaataaatac cggacgattg tgtccggcct
     1921 gtatctgtct ggttgggccg atacgttcaa taatttgctg atactgctcc aggtcccacc
     1981 aatgtgagaa aaataccagt atgtttccgc cgtcctgcat gttcagaccg tggccagcgc
     2041 tggctgggtg tgcaaagaga acaggaatct ttccggaatt ccagtcacgt agtgtctgtg
     2101 gatcctggtc gaggtgacga ccgcgaggaa atgctttaag caagcgttca agatcgtgtt
     2161 tccagtgata agcaaccagc acaggtgcgc cagctgcttc ggtcagtatg ctgtccagcg
     2221 cctgtagttt ggtgtcatgc agttctgacc aacttccggt gtcatctgtg tatactgcgc
     2281 cgctggcgat ttgcagacac ttcagtgtct ttgccgcggc gttcggtgct tctatgcctt
     2341 cgccattcag ctcgaggaac atttcctttt ccatttcacg atactgctga cgggccttcg
     2401 gcggcatatc cacgcggatt acgttatgga tggggtcttt gatatcgaac cagtcggtcg
     2461 catccagcga gagggtcaca tcggctaacg ctcgctgtat ttcaccctgt gagtgagcaa
     2521 aaggctccag tttggtccag ctctgccccg gaaactgtat cgatttgaac cagcgtgagg
     2581 taaacgcgcc gtaagtgcgc ccgagacgtt gccccaggtc cacaaaccac gattgtcccc
     2641 acaaatctac caggccgttc ggtgctggcg taccggtgag atttatccag cgccggacat
     2701 acttgtgcgc cactttgccc agcgccgccg cgcgcttacc accacctcgc agccggaagg
     2761 attttagccg ggtgctttca tccggaatga cagtaccgaa cggccatcgt tctcccaatt
     2821 cttcaaccag ccagacaagg ttatcgtagt tgatggtgaa cacgcttgcg ttgctgttcg
     2881 ccagcgccgt agagcgcgct ttgacattac caacaatcgg ctgtacctcg atattgcgca
     2941 gatgcttcca tttcagcgct tcatccggcc atgtgcttgc tgcaacgcgt agcggcgcga
     3001 ggaccagtgc ggggcgtgtt tctgcccctg ccataaagag atcttccagc gtagtgagcg
     3061 ttgccacggt tttacccatc cccatccctg cccagatgtt gccgcgcagg gtctcgattt
     3121 gatgattgat gatgagatct tggtagggac gtggtttaaa agttatcatg aagaaaatcc
     3181 ctgattagaa aggtgctctt gaacatcatg aatgtaaaag atttatcaat tacaacggtt
     3241 ctcttggccg ccctttattt tatggctttt tctttttaca aaggatacag ctcattttat
     3301 ggattccctg taagttttat ttctattggg attgaggaaa tagttaagtt ttctgttatt
     3361 gcattaggac ttttgtgcac cttagtcgct ttgctgcata ttgatactga agagaagaat
     3421 attccatggg gggtgtattt atttttcttc attttggcct ctttgctttc atactggaca
     3481 atgtatttat atggcggtag ctcatacctt tatgaaaaat ccataaggga tgtggctacc
     3541 caagcaatac tattgggatt tattagccct atcagtgtca gggcagtatc tgtttttatt
     3601 aggaatgaat ttagaataaa aaacaaaact catggggtta tgttttttat atccatagca
     3661 atgcttcctt cagtccttgg gtgggcatgg gcatacttat cagatgagcc tcttttttat
     3721 tctaaaaaat atgatgctta tattatcgaa agctataacg gaaaatttgt cttggggaac
     3781 tgtaaaaaaa aatagctctg aatttatgag tgtcgaagga attgagggga aattaattcc
     3841 tgtaagcata aaagaaacac aacaactaaa agtatgtttt ttaaaagctt ctaagctcaa
     3901 gaaaaatcgc tcagaatgat tgtctcacaa tatcccctct aaatttttgc tatccagtac
     3961 taccacggta aagcccagct ttcgcagacg ttcatgctcg cgcaactggt caggccgtgg
     4021 tggtttgccg ggggatttac attcaacgaa aacgatacga ccaccgggta gcaggacaat
     4081 gcgatccggt accgagcggt gaccgggaga tacaaactta aaggccactc cgccagcttt
     4141 tttcacttca gcgacgaggt gcttttcgat aaggctttca cgttcatagg ccatctgaat
     4201 cccactcttc aaaaagcaca ttcaattcca gtttctctgc caacgcgttt tctgcacgtg
     4261 cgccggcaga gtactcccac tctttgagca tataaatcgt atcggcacag cgaagcattg
     4321 cgaggcagat gtccatgtac tgagcctgtg tcaaaccatc gggtaaagtt gcggggttaa
     4381 gcacaacata tcccttgtcc gttagcctct ttgctgcgga atgaaaagcc gggcggttaa
     4441 attgctcgta accgctcatc ggcccggcaa cgtaaacgat cattcttcga ccaccttacg
     4501 cttttcacgc atattctgca tcaggcaaaa atcagatctg cgttcactcc attcctgatt
     4561 caattcgtga tgtgattcgc ggttggcttt tgcccagacc ttcgctgccc ggtcatattc
     4621 gccggattgt tcaagtcgca aagcctccct tgcggccctg taataaagtg gattgtcccg
     4681 gtatttaaat gacataggag ttaatcctta cggtagtggt acgcctcaaa accgccagcg
     4741 ttcagtggga tatcgggtgc ccattcgggg ttagtggaga gaagcgcgga aagcgcttta
     4801 tcgttgaaat cttctgtgtc aggtgcttcg gtgatcacct cgtcgtgtac cgtcaacaca
     4861 atgctgtaac cggcatcttc gataagcggc atgtttccgg ccagaacgtc gcgggcggct
     4921 gcctgggtga cgttctccac cagctttccg ccgtaggttt tgagtcgttg ccatttacgc
     4981 gaataagagt taacacccat gtaggtgata ttcccttttt cgataaccgg agacgggtag
     5041 catacagcgc gtccggatgg tagctgtatg cgcagccacg cgccatcacg tcggatttta
     5101 agatagccgc aatacaatgt tttttgcggt gtggcgattg ctgtgcggac ggtgcgctcc
     5161 agttcgtacc agaaatcgca ggtcgccggg tgcgccctgc gccagagacg tttaagtgag
     5221 tcgcaggcga taaatacccg ttcagaaagc ccgtaggttg acttgcgttt aaccgattcg
     5281 tcgtaccagc ttttcgcctc gcggataaca tcgcggggaa tgtttggtag tgcggcgttc
     5341 gccagctcgt cgagatcgag accgtaaacc agtgcaaaag tgatgaaggc cgacacacca
     5401 ccaccataac ccagaccgag ttccatgact ttaccgatct gacgcatgtg tttatcaaca
     5461 tcatctggtg caatatcgaa agcttttgca tacgccagtt tatataagtc cggacccgtt
     5521 ccggcgtcgt actctctgaa tgcattcagt ttccattctt ctcccgccag ccatgccagc
     5581 atacggcctt caatgttcga caagtcactt accaccagct ttttgcctgt tggcgcgata
     5641 atgcagccac gtaacgctga actggttagt tccatgatat tgtcaaacag caggtctgca
     5701 catccggctt tcagtgcttc gatgccttcg tctatttgtt cctgttttag tgaagggcgg
     5761 ggaaggttct ggggctggaa tagccgtccg gcccaacgac cggtacgtga cgccccgcag
     5821 aactgtagcg taccgcgtaa gcgcccgtcg tggcttacgc ctttcatcag tgccttgtat
     5881 ttactggtgc tggtagtact ggcctgcagg cggatagcca gcagttcttt cacggcagat
     5941 ggtaaatcgg ggtcggcaat acgacgttcc agagtactgc gttgcatgtc tggtagctcc
     6001 acaccgtagg attcaacaat gtgcttaatc aacgcgtctc gttgtgtggc tgcctgcact
     6061 tcgccatcag tcatttcctg tgtacgcttt gccaggcgct tttgttcctg gtctaccgcg
     6121 tcgatcgcag cgcgtgcgag ttccacgtcc atgcagacgc cccggtcatt gatctgctga
     6181 tcacgatgcc agagcgccag ttctgtcccc tgataattcc acttcggcag acgtttatag
     6241 acttcgcgca ttgcctcgat atccagtccg gcgtaagcaa caaagcgccg ccattcttcc
     6301 gggtgggttt tgctggtggc ccggcgcagt ttgctgtttt tcgggcgtgg cttacagaac
     6361 agctggatca gcgctttacc ttctttgtcc ttcgctttgt cttgcgggac gcctagtact
     6421 tcgcagagtt cccccagaga ccccgggaga ccgtgcgcca gcgcctgcac catcgtgtcg
     6481 cgccaacgtt cgactggcgg tgccagtcgc ggcattgcat aacgcaggac ggtgcggtcg
     6541 aagtgagagt tatgaaaata aagcagggtt tcaggggctg cgattgcttc gtataagccg
     6601 tgtggaatac caccaccggc agtgatatcc catacgttta ctggcccgtc gttgatagcc
     6661 catgcgaaca gcatcacttc aacgccttcg gcatacgcat gggtaccgtt cgtaataggg
     6721 atttcgcaat aggtttccag gtcgccccat agtatattgg acatgtgttt accttggttt
     6781 ttattctatt aactgattaa aagattaatt at
//
LOCUS       DAZFYC010000193         6742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFYC010000193 DAZFYC010000000
VERSION     DAZFYC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.195"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(177..1106)
                     /gene="fdhE"
                     /locus_tag="ACHKW5_004860"
     CDS             complement(177..1106)
                     /gene="fdhE"
                     /locus_tag="ACHKW5_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO7514068.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1103..1738)
                     /gene="fdoI"
                     /locus_tag="ACHKW5_004861"
     CDS             complement(1103..1738)
                     /gene="fdoI"
                     /locus_tag="ACHKW5_004861"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7514069.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1735..2637)
                     /gene="fdoH"
                     /locus_tag="ACHKW5_004862"
     CDS             complement(1735..2637)
                     /gene="fdoH"
                     /locus_tag="ACHKW5_004862"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO7514070.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2650..5700)
                     /gene="fdnG"
                     /locus_tag="ACHKW5_004863"
     CDS             complement(2650..5700)
                     /gene="fdnG"
                     /locus_tag="ACHKW5_004863"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5113..5115),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7514071.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5894..6727
                     /gene="fdhD"
                     /locus_tag="ACHKW5_004864"
     CDS             5894..6727
                     /gene="fdhD"
                     /locus_tag="ACHKW5_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO7514072.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1465 a         1833 c         1835 g         1609 t
ORIGIN      
        1 ttacgcttgt catcagtgac ataccaataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacaggc actggcggca tagcttgagg tggtaacgcg gaatacggga gatggtgtcg
      121 tcacacccta tgggctgtaa tccgggcgac ctgcgccacc cggaaaacgt cagagattac
      181 tccccttcac ccgggaacag gaacgggttg atggaactgc gggcgtagcc ttcttgctcc
      241 attcgcgcgt ccagtaccag agaggcgagg tcatctgcca cggcttcaac tttcggttct
      301 ttttcctgat aaagaatctt caggtaagtg ccgcagtcat cgcagctttc ggctttaatc
      361 gcggcctgtt cgtcatccag cgaccagtaa tgcagtttgc cgctctgttc acagttgctg
      421 cattttacgc gcactacgtg ccattcggtt tcacacaggt tgcagtgcag gtaacgcaga
      481 ccctgagtgg tgccaatttg caccatgctg gacaccggca tagaaccaca aaccgggcaa
      541 tattgacgtt gctcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc
      601 gcccagtaga gcgacagtgc agcccagata aacggcgctt tatcgctgct gacggatgag
      661 aaatcagagg caaacagtgc gctggccata tcttccagct cctgagtcga tgccttctcc
      721 agattctcaa tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaatcagc
      781 gccatcagca gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc
      841 ttgccctgtg cgctggcttc tttaatgcgc gcggtcagat ccatctccag cggatggtcg
      901 tacagcacca cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccagc
      961 ggattatttt ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc
     1021 cgagggaaca ataacggcgg aatcatatcc gccgtacgtt tctcgctcga acccagctca
     1081 tcttgcggga ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac
     1141 ggtaccagcg cgggtgatgt ttcttcgccc aggcgctggt aacccatcct tccaccatgg
     1201 cggtaatcgt gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta
     1261 acgccactgc ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag
     1321 caggcgcaaa ataaggacgc cagataatca cgccgctcac cagcagcaga accaggaaaa
     1381 taatcgccgc ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt
     1441 cctcgttgac gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta
     1501 ggttgtgatg ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga
     1561 caaacgggtg cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga
     1621 aggacgggaa caaaaagccc agcccgctca ccgccgccag gatgaagcag aaggcggtga
     1681 tccagtggtt gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt
     1741 gcgctcctcg tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc
     1801 gacacccacg tagtggaaga tactggccgc aaaggtagcc gcaaagccaa cagccgcgag
     1861 cggtttccag atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa
     1921 gccatgatac agatttggct tgtcagcatg gtgcagcacg tacatgacgt gtgtaccacc
     1981 gacgcctgcc ggatcgtaca gacccgcatt gtcgtaaccg cgggttttca gctcagcaac
     2041 gcgctcgctc gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg
     2101 gcaggtcttc acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acagcgtaca
     2161 tttgtagacg cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat
     2221 gcaataaccg cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg
     2281 aataattgca ccttccgccg ggcacgcttt caggcagcct ggatcggaac agtgcataca
     2341 gccgtctttg cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat
     2401 taccgtccac gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc
     2461 gacggtatcg cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat
     2521 acaggtggtg acgtcgatga gtttcgccac ttcttcctgg aagtcccgtg cctgaggcgc
     2581 gggggtcaga ccgttagtcg cggaacgacg aatgatatct tgagattgat aagccatatg
     2641 tcgtctccgt tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt
     2701 gttcgcatca ccaacgaatg gcgttaacgt attggcaata aagccttttt tagctacgcc
     2761 ttcataaccc cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag
     2821 cgtacgaata cgtttggtca ccaccgcttt ggctttgata tagccacggt tggaggagac
     2881 tttcacggta tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac
     2941 aaactgctct ggttgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaagtg
     3001 ctcggtcaga cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc
     3061 ggcgtcgtct ttaaagatac gtgcagccgg gttcgagata acgtttggat gcagcgggtt
     3121 agttcccagc ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt
     3181 atcgagggca aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct
     3241 acccggaggc gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca
     3301 tttcagcaac tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt
     3361 atacagaatg cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc
     3421 agacggatcg gcgttatcac gacgcgccat ctggttgcct tccggcgtcc agctaccggc
     3481 gaaaatccag cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgagctaag
     3541 ttgttggcct ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc
     3601 cttaccgttg ctctccatcg ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt
     3661 ccaggtcatg ttcagcacct ggtccgggtt cgcgccaccc tgttcggcat acatcttgcg
     3721 caagcgcagg aagataccgg agaggatctc gccatcagtc agcgcaatcc ccggggcgtc
     3781 cgcacctttc cagtgccact gcaaccagcg acctgagtta acgattgaac cgttctcttc
     3841 tgcgaagcag gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc
     3901 gttcagctca ccgtggttct gccagaagtt agaggtttca gtgttcagtg ggtcgatggt
     3961 gacgaggaac ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc
     4021 aacagggtta aagccctggc agatatagcc attgaccttg ccctctttca tcatctcgaa
     4081 gtattgcagg acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt
     4141 ttccgccgtc gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta
     4201 gttgccccag tagtttacct ggccttccag cagcggtttt ggcgtgttgg cggtaaggta
     4261 ggtttgcaga tcggtctgct tctcgcttgg cagcgtcatg taacctggca ggctctgcga
     4321 cagcagcccc aggtccgtca gcccctgaat attggagtga ccgcgcaggg cgttaacgcc
     4381 gccgccggcc atccccatat tgccgagcag cagctggatc atcgccatcg tacgaatgtt
     4441 ttgcgcacca acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc
     4501 gtgagcactg gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc
     4561 acagatgttt tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca
     4621 cacgcagcgc ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc
     4681 ataagtccag gaggatttat cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc
     4741 atcttcaaag ccgtaatcct cacgcactat caggctggcg ttggtatagg cttcggtgta
     4801 ttcgcggttg aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat
     4861 gtcagtaccg gaacgaatag gggcatagta gtcagctacc gccgccgtac gcgtaaagcg
     4921 aggatcgatc acaatcagct tcgcgccgtt gtgaattttg gcttccatcg cccagcggaa
     4981 cccgaccggg tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt
     5041 gatgtcgacc cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac
     5101 cgttggtccg tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga
     5161 gaatttttgc gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt
     5221 ggagagccag cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg
     5281 gtcttctttc atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca
     5341 tttatcagaa cctggcgcac ggtattccgg aaacttcaga cggctttcgg agtggatgaa
     5401 atccaccagg ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc
     5461 gccttcgata tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat
     5521 caacagccca cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag
     5581 cagtttatac tgccgggttt ccgcgagcgc tacgctgggt gcaaaaccca gtgccgctgc
     5641 cgtggtgcct gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat
     5701 ggattgctcc ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag
     5761 ggagtgggag ttatttttct ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt
     5821 ccctccatcc aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc
     5881 tggttaagag aaagtgaaaa aaacacagca aaaagaaatc gaaaatgtga caaatatcac
     5941 aggtgttcgt caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga
     6001 ggtcgcggaa gaagttcccg ttgcgctggt ctacaacggc atttcgcatg tggtgatgat
     6061 ggcgtcgccc aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggataat
     6121 agaaagtccg cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gccttgaagt
     6181 acaaattgag ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc
     6241 gggacgtacg ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt
     6301 gcagccgcta ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca
     6361 tctcaacgat tttcagccag tggggcaact gactggttgt actcacgccg ctgcctggat
     6421 gttgccatct ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga
     6481 caaactgtta ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt
     6541 ttccagtcgt gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt
     6601 gtttgcggtg tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac
     6661 actggtaggt ttttgtaaac cgggtcgggc aacggtttat acccatccgc agcgtttgag
     6721 caattaatta aaataagtcc tg
//
LOCUS       DAZFYC010000194         6556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFYC010000194 DAZFYC010000000
VERSION     DAZFYC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.196"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            73..1095
                     /gene="istA"
                     /locus_tag="ACHKW5_004865"
     CDS             73..1095
                     /gene="istA"
                     /locus_tag="ACHKW5_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFO7514073.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1092..1874
                     /gene="istB"
                     /locus_tag="ACHKW5_004866"
     CDS             1092..1874
                     /gene="istB"
                     /locus_tag="ACHKW5_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFO7514074.1"
                     /translation="MMMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQKEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILPHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            1913..2065
                     /locus_tag="ACHKW5_004867"
     CDS             1913..2065
                     /locus_tag="ACHKW5_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514075.1"
                     /translation="MDHFSSVVDICGQSFWLAVQGMFSGYRVPQSRDIAPIVTFQIDR
                     GLKMWH"
     gene            complement(2803..3405)
                     /locus_tag="ACHKW5_004868"
     CDS             complement(2803..3405)
                     /locus_tag="ACHKW5_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514076.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(3499..3705)
                     /locus_tag="ACHKW5_004869"
     CDS             complement(3499..3705)
                     /locus_tag="ACHKW5_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7514077.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(4832..5032)
                     /locus_tag="ACHKW5_004870"
     CDS             complement(4832..5032)
                     /locus_tag="ACHKW5_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514078.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            5232..5801
                     /locus_tag="ACHKW5_004871"
     CDS             5232..5801
                     /locus_tag="ACHKW5_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7514079.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            6061..6462
                     /locus_tag="ACHKW5_004872"
     CDS             6061..6462
                     /locus_tag="ACHKW5_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7514080.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVLDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            6450..>6556
                     /locus_tag="ACHKW5_004873"
     CDS             6450..>6556
                     /locus_tag="ACHKW5_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO7514081.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVS"
BASE COUNT         1611 a         1644 c         1748 g         1553 t
ORIGIN      
        1 caatagtgat ccacacccaa cgcctgaaat cagatccagg gggtaatctg ctctcctgat
       61 tcaggagagc ttatggtcac ttttgagaca gttatggaaa ttaaaatcct gcacaagcag
      121 ggaatgagta gccgggcgat tgccagagaa ctggggatct cccgcaatac cgttaaacgt
      181 tatttgcagg caaaatctga gccgccaaaa tatacgccgc gacctgctgt tgcttcactc
      241 ctggatgaat accgggatta tattcgtcaa cgcatcgccg atgctcatcc ttacaaaatc
      301 ccggcaacgg taatcgctcg cgagatcaga gaccagggat atcgtggcgg aatgaccatt
      361 ctcagggcat tcattcgttc tctctcggtt cctcaggagc aggagcctgc cgttcggttc
      421 gaaactgaac ccggacgaca gatgcaggtt gactggggca ctatgcgtaa tggtcgctca
      481 ccgcttcacg tgttcgttgc tgttctcgga tacagccgaa tgctgtacat cgaattcact
      541 gacaatatgc gttatgacac gctggagacc tgccatcgta atgcgttccg cttctttggt
      601 ggtgtgccgc gcgaagtgtt gtatgacaat atgaaaactg tggttctgca acgtgacgca
      661 tatcagaccg gtcagcaccg gttccatcct tcgctgtggc agttcggcaa ggagatgggc
      721 ttctctcccc gactgtgtcg ccccttcagg gcacagacta aaggtaaggt ggaacggatg
      781 gtgcagtaca cccgtaacag tttttacatc ccactaatga ctcgcctgcg cccgatgggg
      841 atcactgtcg atgttgaaac agccaaccgc cacggtctgc gctggctgca cgatgtcgct
      901 aaccaacgaa agcatgaaac aatccaggca cgtccctgcg atcgctggct cgaagagcag
      961 cagtccatgc tggcactgcc tccggagaaa aaagagtatg acgtgcatct tgatgaaaat
     1021 ctggtgaact tcgacaaaca ccccctgcat catccactct ccatctacga ctcattctgc
     1081 agaggagtgg cgtgatgatg gaactgcaac atcaacgact gatggtgctc gccgggcagt
     1141 tgcaactgga aagccttata agcgcagcgc ctgcgctgtc acaacaggca gtagaccagg
     1201 aatggagtta tatggacttc ctggagcatc tgcttcatga agaaaaactg gcacgtcatc
     1261 aacgtaaaca ggcgatgtat acccgaatgg cagccttccc ggcggtgaaa acgttcgaag
     1321 agtatgactt cacattcgcc accggagcac cgcagaagca actccagtcg ttacgctcac
     1381 tcagcttcat agaacgtaat gaaaatatcg tattactggg gccatcaggt gtggggaaaa
     1441 cccatctggc aatagcgatg ggctatgaag cagtccgtgc aggtatcaaa gttcgcttca
     1501 caacagcagc agatctgtta cttcagttat ctacggcaca acgtcagggc cgttataaaa
     1561 cgacgcttca gcgtggagta atggcccccc gcctgctcat cattgatgaa ataggctatc
     1621 tgccgttcag tcagaaagaa gcaaagctgt tcttccaggt catcgctaaa cgttacgaaa
     1681 agagcgcaat gatcctgaca tccaatctgc cgttcgggca gtgggatcaa acgttcgccg
     1741 gtgatgcagc actgacctca gcgatgctgg accgtatctt accccactca catgtcgttc
     1801 aaatcaaagg agaaagctat cgactcagac agaaacgaaa ggccggggtt atagcagaag
     1861 ctaatcctga gtaaaacggt ggatcaatat tgggccgttg gtggagatat aagtggatca
     1921 cttttcatcc gtcgttgaca tttgtggcca gtctttctgg ctggcggttc aggggatgtt
     1981 ttctggttat cgtgtaccac aaagcaggga tattgccccg atagtcacat ttcagattga
     2041 tcgcggtctg aagatgtggc actgaggtaa cgtgctggtg tcactggtat tgctgaagtt
     2101 atggtttttc ggggtcaggt gagcctgttg tgtgtatgct ttttgttcag gtcagagggg
     2161 gactgtgtgg acagcggctt ttgtctccgg gcattcacgc ctgaacgatg aatgcgattt
     2221 tcagcaatat ttgtctttgt gacactctct ggttgagaag ctaatgcgcg cccctgtttc
     2281 cacagagaat gaggcttgcc atcagccgaa acatcagtat tccacgccat acagacggcg
     2341 cctgaccatg tgctgatgca ggtaaaatgt ttcactacaa ctgttcgagt tgctatgcaa
     2401 cgaaacagcg attctcacga tctttaactg caacgttttt ccgcctgaaa gctgactgcc
     2461 tcctgctgag gtgggaggct atgattcagt gcgtttatag tctccggggt atgcatgtac
     2521 cggagcatac catttctggt ggataacaag ccggcacatt cagactgcag tgaagatgga
     2581 ggcacctggt tgtgaagaac gtttctgccc gtggctgctt cgcatcatca atggtattca
     2641 tacccgctca gaaatgaccg ggtgctgaag ccctccgtta tcgcgcagta taatgacgta
     2701 tatgatctgt ggacgagtga gcggcgtgcc tgatgtacgc cgctattact gtattttacc
     2761 acagggctgg ttttctgctg ccagccctgt ggttctcaga catcaactgt gagcggcatc
     2821 aaccgatgta cggtatgcca taagcacgta ctgatccatc agatttgtca gtggttcact
     2881 ctggccctga cgtgcaatga tccattgctc cagatctttt tctgatttgt gtttcaggtt
     2941 tttcgtccag tcgtcaaggc tgtttccggt cattaagtca agccagcaga gccagaccag
     3001 tttgagacgt aactccgtgc cgccaaactg acggaagagg ctgatcagtt tgtttgccag
     3061 agaatgttgc ttcaggcgct cacattcaga cagcaggatc ccttccagca tcggatgatg
     3121 aaccagcagg tgttcgctgg tgcataaatt caccaccaga tcatcatcca gggacgggga
     3181 gctccgtttg tccggaccat aaatcagccg gtgcagactc tggggaacga tggcaatctc
     3241 cggtttttcg ccattgaatg acacccaccc gggcgtcaga ttatggaaat agtagatatt
     3301 cggatgacac acgccccatg atacggcgtg ccgggtgaag ccggtcagcc atttctggta
     3361 gttttcatgg agcagctcgt aacgggtctg atactgttgt ttcattttca tattcctcac
     3421 gggggataac aggcagcaga gcgccgccgg caggccggag attgcgtctg ctgtgggcga
     3481 agggttagtg gttaaaaatt acggacggga ctgtgcaata cgcgcctgca gccaggcttc
     3541 cacttcactt ttcagccagc gggagctgcg ccccattttg atgggcgcag gaaagccccc
     3601 atctttgatg agtttgtcaa accacttatc ggttaggccg gtcagttgag tgataaacgc
     3661 catgtcgacc atctggtcat ccatcagcga aactggggta gccatgagaa tgttcctccg
     3721 gatgttgtta aaccagccgg tgtggtcatg aaaacagcgc gccaccttca ccggcataac
     3781 aaccggggag aaacgtaaaa aataatcggg aatgcggctg tccggagata aacgggggga
     3841 ttgcgatggc agagccgtag cggaatcccg gaggggggtg ttgcgaaaag atgcttccgc
     3901 acgcacgcgc gataaagagg ggatagtttt cgtgaggaca gggagagaag caggcgttct
     3961 gataattgca gagttgtttt cggaggtggt tacagtgagt cagcccgttc ctggcgagca
     4021 ggagactgat atcaccgatg ggataaggtt gtctgattgg tattataaat ataagaagtc
     4081 atcagagagt aacgtaaagg atttgccaga ataacctaag agagtaaatc tctccggtaa
     4141 cagagcgctg tgaagcattc tgtatcagag tgcagataca tgaacgctga tgcgtaaaat
     4201 cagcagtgac acgccagctc cccctatgcc cattcctgta taagacatga actgagtgta
     4261 ttaatgcttc tgccctgaga ctgagccggt gagtgagggg ctgagaaatc cctcttgaca
     4321 cagctctgac cagaaatact tccccggttg ttgcggtgcg tccggatccc cgataacgga
     4381 acactaaggc tgtattgcgg gacaccgggt aagtagcagc ccgtaaggcg ttctgtcaac
     4441 gccagaaaaa atgatttccc acacgcgtta tagactcact cctgtggacc tggaagcccg
     4501 taactctgcg gttgcccact gactgacgga actgaccgca aattttacga gtgcgtgctg
     4561 ggcttcagtc tggtcacctg agcgttcggt aacggaggcg cgggcggtaa taggctgatt
     4621 ttcaggtatt accggcttac agcgtgtggt tgatattgag gggctgtgag tttcgcgtat
     4681 ttgtcagaaa cccagacgag ttttcctgta tgggacgaaa taaggcagcg gcaatacctg
     4741 gaaaaattta atatggtatg aaggagaacc tgtcggttac gcttaatata cccggtacac
     4801 tgtcagctca ggggatacat gaaccgttat ctcaccagct acacagtata tacacactga
     4861 cgctgacagt agtcagcagg accacaataa tcagtgccag cagtatggta acgatacggt
     4921 ctgtattccg gtaatagccg aaggggtcga cagaaccaca atacgggcaa cacactgtat
     4981 tatgagtaat cgcgtttcca cagtccctgc acttcttttt gcgtatcacc atcactccct
     5041 cctcacagcc ttacccgtaa ccggtttttt acattaaaaa aacaacgctg aaaattcaac
     5101 cgtcagtccg gagacgaccg ttcgggttat cacagagtgc ctgagacagt gtcctgccgg
     5161 aggtcacttc agggactctg cgcatttttt acgacgtggc tattccgtat gaaccatacg
     5221 gagatttaac catgacctac aaatacaacc ccttctggca gcaacgtatt cgtgagacgg
     5281 tgcggcacgc actgaatgtt catccccgcc tgacggcatt gcgggttgac ctgcgtttcc
     5341 cggatgtacc ggcagcaacg gacgcagctg tgatatcccg cttcatcaat gccctgaaag
     5401 cccgaatcga cgcttaccag aaacgtaagc atcgggaagg taaacgcgtg catcccacaa
     5461 ccctgcatta cgtctgggcc cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc
     5521 tgatgctgct ggttaaccgg gatacctggt gtcgtgccgg tgattaccgt gctccgggat
     5581 cactggccgg gatgattaaa caggcgtggt gcagtgccct gggagtggat gtcgggtgcc
     5641 atgccacgct ggtgcatttt ccggcctggc cggcggtgtg gctggaacgc gatgatgaca
     5701 ctggctttca gcaggtgctg gaacgtgctg actatctggc gaaggaacat accaaagctc
     5761 actgcaccgg tgagcgcaat tttggctgta gccggagttg agcacgactg gcgcgccaat
     5821 acccggcaaa ccaaaagtat gggttgttgt ctggcggcaa cgatgtatcc actcgcccac
     5881 tgactgtttt ttacgtcagt gttccattgt gtgacaacca cagtgctgta acagcaacca
     5941 gcctgcttta cccaaactcc agtccagatt ttaactgaac cgccattcac accctgacca
     6001 cgctgcccgt accgggacgg ctgttgcctg cgtgtcgtct ggcggctgag gaatatgact
     6061 atgtacgcaa aatcctttat cgctcttgat ggcaacggac gtctgacggg cgcccgtact
     6121 gcacaggccg caccttatgc taactacacc tgccacttgt gtggcagtgc actcagatac
     6181 catccgcaat acgacactga acttccctgg tttgaacaca ctgacgacag gctgacagag
     6241 cacggtcaac agtgccctta tgtcaggccg gagcgcagag aaatacagtt gattaaacgt
     6301 ctgcagcaat tcgtactgga tgccttaccc gtggtgcgta aagccagctg gcactgcaga
     6361 caatgtcacc acgattatta tggggagcgg tactgcacac actgccagac cggaggattc
     6421 agtattcccc ggacaactca ggaggaaata tgcgaattct gaactgttat atggcgaatg
     6481 acagcaaagg ccattttgtt acggcgaaag aagcagcgaa gcacaaccgc caggacgttt
     6541 tgtgctgtgt gtcctg
//
LOCUS       DAZFYC010000195         6385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFYC010000195 DAZFYC010000000
VERSION     DAZFYC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.197"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(168..380)
                     /gene="cspH"
                     /locus_tag="ACHKW5_004874"
     CDS             complement(168..380)
                     /gene="cspH"
                     /locus_tag="ACHKW5_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO7514082.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            666..878
                     /gene="cspG"
                     /locus_tag="ACHKW5_004875"
     CDS             666..878
                     /gene="cspG"
                     /locus_tag="ACHKW5_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO7514083.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            889..1077
                     /gene="ymcF"
                     /locus_tag="ACHKW5_004876"
     CDS             889..1077
                     /gene="ymcF"
                     /locus_tag="ACHKW5_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO7514084.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1052..1282
                     /gene="ymcE"
                     /locus_tag="ACHKW5_004877"
     CDS             1052..1282
                     /gene="ymcE"
                     /locus_tag="ACHKW5_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO7514085.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFREM
                     AYNISYLTRGPGTNTLLACCCLLRPEEVNSEY"
     gene            1272..1445
                     /gene="gnsA"
                     /locus_tag="ACHKW5_004878"
     CDS             1272..1445
                     /gene="gnsA"
                     /locus_tag="ACHKW5_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO7514086.1"
                     /translation="MNIEELKKQAETEIADFIAQKITELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1494..2567)
                     /gene="yccM"
                     /locus_tag="ACHKW5_004879"
     CDS             complement(1494..2567)
                     /gene="yccM"
                     /locus_tag="ACHKW5_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7514087.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNNWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETANSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPTIGH"
     gene            complement(2639..5383)
                     /gene="torS"
                     /locus_tag="ACHKW5_004880"
     CDS             complement(2639..5383)
                     /gene="torS"
                     /locus_tag="ACHKW5_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO7514088.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARKLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCLCLRLPLRVATAPEPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLVQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANTDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMCSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            complement(5478..>6385)
                     /locus_tag="ACHKW5_004881"
     CDS             complement(5478..>6385)
                     /locus_tag="ACHKW5_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514089.1"
                     /translation="SDSGRKSLIDRIKGGDAITLHVWLDRYETILTERGIRPKTLLDY
                     ASKIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEG
                     HVATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDL
                     CRMKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIAS
                     KHYDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLG
                     HKSDSMAARYRDSRGREWDKIEIDK"
BASE COUNT         1557 a         1594 c         1533 g         1701 t
ORIGIN      
        1 gcggtactat aactggctgt taagtggcat ttataactat tagttgcaga gatattcggt
       61 taaatggatt agtttcctga taaaaaagta acttgcgtta taatgcatgg tgttgactat
      121 tgaactattc caataaaacc agtcagcttt aaacaagcag cgtcatatta agagagataa
      181 acatttgccg ctgttggtcc tcgcaggcca tttacgcgac aaaattccac gcgtaatcct
      241 ggtataagca cttctgcgtc gcggggagtg aatgcggaaa tatggacctg gacttcttta
      301 cgaccgtcgg aggggataat gaatcctttg ccgcttttgc gatcaaaggt tttgacaatt
      361 cctgtcattt tacgggacaa acaaattcct tactgaaaat actgcgctgc actatacggg
      421 gttaataaaa taaagccagc gaaatttaag accgccggac ggctaaaata aaatttgctt
      481 aatctcaatt atcatgcgtt aatagctgcg tcggtttgaa agacagacag catacaaagt
      541 agtttactaa agcagttctc attatcaggc attatcccct tcttttgagt ctctctcctg
      601 aacactaagt agtttctgta ttaaagccct gtttgccgaa aggcccaaaa tgaaggaagt
      661 aaaatatgtc taataaaatg actggtttag taaaatggtt taacgcagat aaaggttttg
      721 gctttatcac tcctgatgat ggcagcaaag acgttttcgt ccatttcacc gccatccaga
      781 gcaatgaatt ccgcacgctg aacgaaaatc agaaagttga attttctatt gagcaggggc
      841 aacgtggccc cgcggcagcg aacgttgtta cgctctaagg ttgccgttat tactcaacat
      901 ctccatttcc gctgtccatg ttgtcatggt tcacagtacc gcacatcggc attcgatgtg
      961 acggagcgaa accctttggg cgctaagtgt attttttgta aatcgacaat gatcaccttt
     1021 gataacgtcg cgctgcaaat acgcactgac catgcgccgc tggatttcac aaaataatat
     1081 caggctccct cgtggagcct tttttatatc tgccttattt ttcttcaacg ctgtatgtat
     1141 agtaagcgat aacctgttga ttattgaatc tttcagggag atggcttata acatttctta
     1201 cctgaccagg ggaccgggaa ccaacacctt actggcgtgt tgctgtcttt taagaccaga
     1261 agaggttaac agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa atcgccgact
     1321 ttatcgcgca aaaaatcacc gagctgaaca agaatacagg gaaagaagtc tctgaaattc
     1381 gcttcaccgc acgggaaaaa atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa
     1441 tgtgattttg tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct aatttagtga
     1501 ccaatcgttg gcgcttgtgg aattaagcgt cggtacaatt cctccggcac cgggctttgc
     1561 catactcccg catacattgc gtaaccaatc accgcaaaca taatccccag aaccagtagt
     1621 gtcattaacc agccagacaa cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa
     1681 aaggtcaatg ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc
     1741 actgttcgta cctgaattaa tttatcgacc gggatccgtg atgggcaatt ttttgcgcat
     1801 ttgccacagt cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa
     1861 accacgccca tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac
     1921 aggctggcaa tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag
     1981 aaatcgagca ttttaacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc
     2041 gcctgagcgg gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg
     2101 ccgcgcagag gaatatccag ccagcgggga aggacacatt gccgaccaaa cagtttgtta
     2161 ccgagatcgc cgattaattc agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc
     2221 tttttgagta atagactgat gacgataaaa gcgaccaata acagcatcgc ggcggcgtgg
     2281 acggacggta actgacctgt ggtaaggcta tatttcagat tcatcagacc ggcaatcggt
     2341 agccagcctt cgatacctcc cggtctggcg acaaatgtcg tgctatttgc cgtttcgtaa
     2401 tagcgcaccc aataccagaa cgtgatggca atataaatat tcattgccaa cagtaataat
     2461 tgcgtcgctt tacgccaggt cgtggcattg cgccagttat tccacggtaa tttgccgccc
     2521 gtcgtgcctg gccgccgctg ccagcgggtt cttttattct ctgccatgat tttgccagtc
     2581 cgttaagttg cataccaaat gccactattc tagttgttct taactggctg atattgattc
     2641 aaatcgcgtt caggtctttc ttatgcaacc atgcttccag agcggcaaca ctgcgtgtaa
     2701 tttcttcgtg tggaaggggg gcagataatg gctgctgctc cagttgtgcg catagctggc
     2761 tggcactgca cattcccaga ctcgagcaac tgctttttag ctgatgtgcg gcacgcttta
     2821 ttttctcgct atcctgactg gcgcgggcaa tgtcgatttc atcgagaagc ggcagggcat
     2881 gttgtgtaaa taataccagc cattcgtgga tcttctccgt ccccattaac tgagcatctt
     2941 cattgagttg cgatacatcc agcggttgat cggtattggc ttgcagttgg agatagtgcg
     3001 ccagtaactg accgagcact tcacgcggca ccggtttagg gataatcccg cgaaataatg
     3061 aactggtacg ctggcgcaac gtttcgtcga tgacatgagc gctaaagcca atcaaaacca
     3121 gcgacggata ttgctgtacc agttgtcggg caagggtaat gccgtcgata tccggcagat
     3181 caaaatccac cagtgcggca gcaaacggtt cgctattttg cagtgtctct aaagcctgcg
     3241 cggcattgcc aacagcaaca acctgcgcac cacttgtgtt cagcatctca acggtaattc
     3301 gctgggttag cgggttatct tcaattaaca gcaaacgtaa accgtccaga cgcaccgcct
     3361 gattggctgt ttttggctcg ggtgcagtgg caacacgtaa cggcaagcgt aaacaaagac
     3421 agcttccaac ctccggcgtg ctggtggcgc tcagttcgcc gcccatcgcc tgggccagac
     3481 ggctactgat agtcagcccc agcccggtgc cgccgcgttt gccgcttacc tgcacaaatg
     3541 gctggaagat ttctgccagt ttcgcgggat caataccgca gccgctgtct tccacttcga
     3601 ccagccattg ctcgccatca gtgcggctac gtaaaacgat ctgcccttcg tcagtaaaac
     3661 gtagggcgtt gctcaacagg ttggttataa cctgacgaat acgtcgcgga tcgcccatta
     3721 acgcgctcgg tacatcatcg gcaattgccg ttgccaggcg aatcgggcga cctttcaccc
     3781 gtccgctcat taattgcagg gtactttcca gcagcgggcg cggttcaaag ggttcatcgc
     3841 tgaccgaaac attcttgccg cctgcttcga tagccgaata atcgagaata tcgttcagga
     3901 tggtcagcaa cgattcacca gagtcagtaa ttgcccgcaa atcatcacgc tgggcgttaa
     3961 gtgcggggtt atctgccagt agctgtgcgg tgccgagaat accgtacagc ggtgtgcgga
     4021 tctcatggct catcgccgcc agaaacgccg attttgcctg gctggctttt tctgcttccg
     4081 cccgtgcctg gcggtgttct atcaccagtt cctgcaattc agctgtacgc gctttgacct
     4141 gcgccgccag ctgttcgcgg tggcgattca gtgcatgaac attgctgcga aacgcatcca
     4201 tcagccgccc gatggtatcc agctcccgta cgccagcggt ttccgggaaa ggggagtcaa
     4261 tatcaccgtc cagcagccgt tgcagcgcct gcgtttgttc ggcaagtgga cgcgtgactg
     4321 agcgataaac cacgcgccaa aggatcagaa tcagtgcgca aagtgaaacc atccccagca
     4381 ataacaggct gtattgcccg cgtgcactgg ctttttccag atgcgccagt ccgtgctgat
     4441 tacgcagctc aatggtgtcg accagctgac tgacttcgct actaaactgc gcgaactggg
     4501 cgatgttatt ttgtgcgaga gtttgtaggt gattgctgat ttcactgtcc tgctgataca
     4561 gcgccagcaa atcgctatat tggctaacgg tagttaacgt tgttgcgacc tgcgcacgaa
     4621 cacccggatc ttcaatgcgt atttgccgac gttgcagaat tttcaccgca ttattgagct
     4681 gcttttccag cgttggtgca tttttctgga tctgctccag ccccagattc atcaccattt
     4741 gctgcacccg cagagcgcta aggcgcagtt cattcatctg gttaacatac tcaagatcga
     4801 tatcaatcag ccgatcgagt gcactttcag cagcctgacg ctgatcttgt tcgatcaaat
     4861 cgtaaatccc ggcctgggtc gctccagcgg aagttgtcgc attattcgcc tgaccttgcg
     4921 ccaggcgtgc gatctcatcg gcggcagcga ctatctgctg actgagttgc cgttgttgct
     4981 ggcgtagttg cagacgccgc cccaccagtt ccccttgctg acgtaatgaa cgggagatct
     5041 cctgctcctg ttgttcaata gcggtggtat caaaaccttg ttcccgtaac gcttgcagca
     5101 acgcattaat cttcaggctt tgtgcggtga gcattcgccc ctgcgcctgc cacatctttt
     5161 cgttatcggc actggtcagg ttctgcgcgg cgaaaagttc ccaggcgctg gcttcgctca
     5221 acttgcgcgc catattcatg gtaggaatca atgcctgagt gttgtctttt tccacctggc
     5281 tgataaagcg caggttgtac catcccacca gggtactggt cagggttaac agcgccatca
     5341 gggcaaagcc catccagagt cttcgggtca gggttaaatt cacggtcggt gcacttaagg
     5401 tgaataagtt gtatgtttaa aatctctttt actatcaatg aattagtatt ggcaggtcat
     5461 tattggtcaa aataaaatca tttgtcgatt tcaattttgt cccattcccg tccacggctg
     5521 tctctatacc gcgccgccat tgaatctgat ttatgcccga gaagacgttg agcaaattta
     5581 tcgccaatct ggttccggta tagcctcgct gacaggctac gcagttcatg gaatgttggc
     5641 gggtttccat caaatgagag tccagatgca tttctcgcct ttgtaaaata ctttgatact
     5701 gttttcgggg aaagaggatc gtaatgcttt gatgcgatta tagtttcact gctgctggcc
     5761 tccctgcatt tctgtagtgt atcagccaat gagatattga gcgcgtcaat cgttagcgtt
     5821 agcggaatgg cgagtttagc ccctgtttta ctctgttcaa tgtgaagatg gttgtcgttt
     5881 atgtctgacc atttcattct gcacaaatcg ccgactctct gccctgtaac gacggccaaa
     5941 tccatcgcca gccttagcca gatagggaga ggttcggctg catggtaaat ctcgacatac
     6001 tcattagctg tcagccttga gcgccttact tctgactttg ctgtacgggt tgctgttacc
     6061 ggattcgttg ccacatgccc ctcggctatt gcttcacgaa aaacgtcaac aagggttgac
     6121 ctgattaatt ttgcggaagc tgctttacct tctgctacgt aggtgtttag cattgctgcc
     6181 acttctttcg ttgatatgtc agtgagcggt ttgtccggca attttcttcg gattgccctg
     6241 attttgctgg cgtagtcgag tagagttttc ggcctgatcc ccctttcggt gaggattgtt
     6301 tcatatcggt caagccacac atgaagagtg attgcgtcac cgcctttaat tctgtctatt
     6361 agtgatttgc gtccgctgtc tgaga
//
LOCUS       DAZFYC010000196         6160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFYC010000196 DAZFYC010000000
VERSION     DAZFYC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.198"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..416)
                     /locus_tag="ACHKW5_004882"
     CDS             complement(<1..416)
                     /locus_tag="ACHKW5_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514090.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWL"
     gene            complement(503..709)
                     /locus_tag="ACHKW5_004883"
     CDS             complement(503..709)
                     /locus_tag="ACHKW5_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7514091.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1412..1610
                     /gene="hha"
                     /locus_tag="ACHKW5_004884"
                     /pseudo
     CDS             1412..1610
                     /gene="hha"
                     /locus_tag="ACHKW5_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
     gene            complement(2025..2225)
                     /locus_tag="ACHKW5_004885"
     CDS             complement(2025..2225)
                     /locus_tag="ACHKW5_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514092.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            2429..2998
                     /locus_tag="ACHKW5_004886"
     CDS             2429..2998
                     /locus_tag="ACHKW5_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7514093.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLVHFPVRPAVWLERDDETGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRS"
     gene            3257..3658
                     /locus_tag="ACHKW5_004887"
     CDS             3257..3658
                     /locus_tag="ACHKW5_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7514094.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTREEICEF"
     gene            3646..4080
                     /locus_tag="ACHKW5_004888"
     CDS             3646..4080
                     /locus_tag="ACHKW5_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO7514095.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRRDVLCCVSCGCPLTLQR
                     GNDGQQPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVLDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQDVPSEDARADNPLQRL"
     gene            complement(5467..>6160)
                     /locus_tag="ACHKW5_004889"
     CDS             complement(5467..>6160)
                     /locus_tag="ACHKW5_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514096.1"
                     /translation="FMTPGIVQILPMLFLLTQLDFSEETSDLVEMLMLLALTAAIVTI
                     GILPGAIHLNERRNGDKFTGFIVVLICIPVVITVFSMWYRPIPNMIINMVMSLSGISD
                     WRTHQYYIENKTHSHSMFNGLIWNTRHYTEIPERFFITGVSTFTLGDIKLVCPTEITE
                     ARKESLKFTINDINEYEQKGKQLKAVAMKCIPFNKNDIHTWDSPLSEPIYYEKVKQTI
                     DNSMLKILHTLK"
BASE COUNT         1608 a         1431 c         1655 g         1466 t
ORIGIN      
        1 agccagacca gtttgaggcg caactccgtg ccaccaaact gacggaagag gctgatcagt
       61 ttatttgcca gcgaacgctg cctcagacgt tcacattcag acaggagaat gccttccagc
      121 atcggatgat gaaccagcag atgttcactg gtgcataaat tcactatcag atcatcatcc
      181 aggggcggcg tggtccgttt gtccggacca taaatcagcc ggtgcagact ctggggtacg
      241 atggcaatct ccggtttttc accattgaat gacacccacc cgggcgtcag attatggaag
      301 tagtagatat tcggatgaca cacgccccag gatacggcgt gccgggtgaa gccggtcagc
      361 catttctggt agctttcgtg gagcagctcg taacgggtct gatactgtgg tttcattttc
      421 atattcctca cgggggataa caggcagcag aacgccgccg gcaggccgga gactgcgtct
      481 gctgcagatg aacggttaag aattacggac gggactgtgc aatacgcgcc tgcagccagg
      541 cttccacttc gcttttcagc catcgggagc tgcggcccat tttgatgggc gcaggaaagt
      601 caccatcctt gatgagtttg taaaaccact tatcggtcag gccggtcagt tgagtgataa
      661 atgccatgtc gaccatctgg tcatccatca gtgaaactgg ggtgatcatg agaatgttcc
      721 tccggatgtt gttaaaccag ccggtgtggt catgaaaaca acgcaccacc ttcaccggca
      781 taacaaccgg ggagaaacgt aaaaaataat cgggaatgtg accgccagga gataaacggg
      841 gaggttacgg tggctgagcc gtaccggtat gccgggtggt tgttacgaaa cgatgcttcc
      901 gcacgcgcgc gcgataaaga agggatgatt ttcgtgagga agaggggaaa actggcgttc
      961 tgataattgc agagttattt acggggcggt tacgatgggg cagaccgtgt ccggtgggca
     1021 ggcgacaggt atcaccgatg ggataagact gtctgattgg tattataaat atataagaga
     1081 gagcaaacac atgagatcaa atacatgagt tcatatatgt gaaataaaca cggtgagtgt
     1141 gatcagtggg catgatgaga acgcagtagc aacagactgc gctatccctg atagctggcg
     1201 tataacctgc gtatataaaa cgtaccagca acgcacgtga acagagtgca ttaagcatcc
     1261 gtcgttgaca ccgacctggt gagtgacagt ctgagagacc ggttaagtgg tggtacggga
     1321 gtaatgagtt tggggagggt accagaatgt cagtctggta cggtatgctg acagtatcac
     1381 gttaattgca gcataaagtg atgagaggct aatggaagtt aaaaccagag cagttccgtc
     1441 gttgttcatc ccgggagaca ctggaaaaag tgatttccca cacgcgttat aagcttactc
     1501 tagcggagat ggaagccttc aactctgcgg tcgatcaccg actggcagaa ctgacaatga
     1561 acaagcttta cgatcgtgtt cccgcttcag tctggaaata tgtcatctga gcgttcggta
     1621 atcacaactg catcgcgcca ggtgttatcc gctttgtgat tcttattggc tcttctcagg
     1681 ttattaaaag ctgaatatgt tcctgttgtc gggaaccagg ataataccgg gaacgttaag
     1741 cggtatagcg acaacaaaga attcgaaatc aaaagggaat ggtgccggag gcgtgggcgg
     1801 taatgggctg attttcaggt attaccggct tacagcgtgt ggttgatatt gaggggctgt
     1861 gagtttcacg tatttgtcag aaacccagac gagttttcct gtatgagacg aaataaggca
     1921 gcggcaatac atggaaaaat ttaatatgct atgaaggaga acctgtcggt tacgcttaat
     1981 ataaccggta cactgtcagc tcaggggata catgaaccgt tatctcacca gctacacagt
     2041 atatacacac tgacgctgac agtagtcagc aggaccacaa taatcagtgc cagcagtatg
     2101 gtaacgatac ggtctgtatt ccggtaatag ccgaaggggt cgacagaacc acaatacggg
     2161 caacacacag tattatgagt aatcgcgttt ccacagtccc agcacttctt tttgcgtatc
     2221 accatcactc cctcctcaca gccttacccg taaccggttt tttacattaa aaaacaacgc
     2281 tgaaaattca accgtcagtc cggagacgac cgttcgggtt atctcagagt gcctgagata
     2341 ttgcctggtt ggagatcact tcagggactc tgcgcatttt ttacggcgtg gttaatccgt
     2401 atgaacgaaa ccatacggag atttaaccat gacctacaaa tacaacccct tctggcagca
     2461 acgtattcgt gagacggtgc ggcacgcact ggatgttcat ccccgtctga cggcattgcg
     2521 ggttgatctg cgtctcccgg atgtaccggc agcaacggac gcggctgtga tatcccgctt
     2581 catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa cgtaagcatc gggaaggtaa
     2641 acgcgtgcat tcctcgaccc tgcattacgc ctgggcccgg gagttcgggg agcttaaagg
     2701 caaaaaacac tatcacctgc tgctgctggt caaccgggat acctggtgtc gtgccggcga
     2761 ttaccgcgct ccggggtcac tggccggaat gattaaacag gcctggtgca gtgccctggg
     2821 ggtggatgcc ggatgttatg ccacgctggt gcattttccg gtcaggccgg cggtgtggct
     2881 ggaacgcgat gatgaaaccg gctttcagca ggtactggaa cgtgctggct atctggcgaa
     2941 ggagcatacc aaagctcgcg gcaccggtga gcgcaacttt ggctgtagcc ggagttgaac
     3001 acgacagacg cgccaatacc cggcaaacca aaagtatggg ttgttgtctg gcggcaacga
     3061 tgtatccact cgcccactga ctgtttttta cgtcagtgtt ccattgtgtg acaaccacaa
     3121 tgctgtaaca gcaaccagcc tgctttaccc aaactccagt ccagatttta actgaaccgc
     3181 cattcacacc tgaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctggcg
     3241 gctgaggaat atgactatgt acgcaaaatc ctttctcgca cttgatggca acgggcgtct
     3301 gacgggtgcc cgtaccgcac agactgcacc ttacgatcgc tacacttgcc acctttgcgg
     3361 cagtgcactc agataccacc cgcaatacga cactgagcgt ccctggtttg aacacactga
     3421 cgacgggctg acagcgcacg gtcagcagtg tccttatgtc agaccggaac gcagggagat
     3481 acgcttgatt aagcgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc
     3541 cagctggcac tgcagacaat gtcaccacga ttattatggg gagcagtact gcacaaactg
     3601 ccagaccgga ggattcagta ttccccggac aactcgggag gaaatatgcg aattctgaac
     3661 tgttatatgg cgaatgacag caaggggcat tttgttacgg cgaaagaagc agcgaagcac
     3721 aaccgccggg acgttttgtg ctgtgtgtcc tgtggatgcc cgttaacact tcagcggggc
     3781 aatgacggac aacaaccgtg gtttgaacat gaccagatga ctgtcgctga gaaaatcctg
     3841 ctgcgatgca cctggcttga cccggcagag aaagaggccc gtcgtttgca tctgcagggt
     3901 atgacggttc tggattatac ggtgaaggtg agaaagtggt tttgtgtgat gtgtgacgaa
     3961 gattatgaag gagaaaagtg ctgcccgcgt tgtggtaccg gggtatacag cagggcgtgg
     4021 gggcggcagg atgtgccgtc ggaagatgcc agggctgata atccgttaca gaggttgtaa
     4081 tggttgcctc tggcgcagtt tgcggtgatg ccgttcccgg gaatggattg ccagtgcggg
     4141 gactgtggag cttaattccg gtgttggtgg cctttccgtt gattttgagc caacagccct
     4201 cctgtgactg acaggctgcc tcgtcattcc atacgtatct tcaggtgaac agatgcatgt
     4261 ctgagcgtac tcacgcagcc ggcccggcaa ccgcggtggt actacgctgg ctgcgagaaa
     4321 atacagaaag caccagaata cgctctcctg ttgctgatca atcaggatct catgcaccta
     4381 ccgggatcta gggctagaac tgatgaaacc gattcgcacc tggctgagtt aatgctacag
     4441 gccagggcat cactaccccc tgacgagcac tgtactgtgg tatgcagtta cagggggcgt
     4501 gtgcagccac acctgaaagc agtgagtcgt tcacggcatt caaaaacacg tgcctttcgt
     4561 tcgccgttcc tgtgatggcc ggcatcattt gctaagccgt taccgacgtt attccctccg
     4621 ggacacgcct ctggttgagg ctttgcccgg agcaatcgaa tgaaaataag ttaagtcacc
     4681 gaaagggagg atgtagtcaa ccgggggaac agtaataagc cgtcgtgctt ttatcgtaca
     4741 agtgtgttgc gttttgcacc gaaaatacga tagaggtcgt ttatggattc cgcagggagt
     4801 gctgattatt attcactggc agcagacagc gatgtgtgtg caggcattca gggggatgtt
     4861 gtcagcagaa gggtgcgtat agcagattgt tgttatacat ggcgtatgaa gtattaccag
     4921 tcatgcggag tagtgtggtg aggggctgta tgagaaatcg ccaccttctt gtcagagagg
     4981 gaacgctacc atgaaagtca aaagcgataa cgcagaccgg tacgatcggt aggtaaaact
     5041 gtagcggtgt gctgccgccg aggtcagaat aatctgcgcg tttttacgcc agcgccaccg
     5101 acgaacagca ggagattgag gggaatttgg cggcgataaa gacgtagaag agaaaaacgg
     5161 gcaatttgcc gcatgcccac agaatcagtg agatcgttag cgacagcaaa atgatcggca
     5221 gccagcggcg gcctgcaata tcgggatgaa ggagatccta cgatatcgat gctgcacctg
     5281 atatgtacga tcagtaaata gagtacgaaa cagaaaacac gacgataata acgctggcag
     5341 cggtgcctgt caggcaaacg cccacacgca ataaaataaa tcaaccatcg cagggccgtg
     5401 atcatccgca tacaccgctg atgacgcgta taaaacgcgt caatatgact tccccggaac
     5461 attacctcat ttcagggtat ggagaatttt taacatcgag ttatctatgg tctgtttgac
     5521 tttttcgtaa tagatcggtt cagataatgg cgaatcccag gtatgaatat cgttcttatt
     5581 aaaaggaatg catttcatgg ctactgcctt taactgtttc cctttttgct catactcatt
     5641 gatatcatta atggtaaatt tcaaactctc ttttcttgct tctgttattt cagttgggca
     5701 taccagcttt atatctccta aggtaaatgt actgaccccg gtgatgaaaa atctctcggg
     5761 gatttctgta tagtgccgag tattccagat taagccatta aacatactat gagaatgagt
     5821 tttattttcg atataatact gatgcgttct ccagtcacta atgcctgaga gactcattac
     5881 catattaatg atcatatttg ggatagggcg ataccacata ctgaatacag taattacaac
     5941 tggtatgcat attaaaacaa ctatgaatcc agtgaattta tcaccatttc tgcgctcatt
     6001 aagatgtatg gcaccaggca gaatgccaat agtaacaata gctgctgtta aggcaaggag
     6061 cattagcatt tcaaccaaat cacttgtctc ctcactaaaa tcaagttggg taagcagaaa
     6121 taacataggt aatatttgaa cgatcccggg agtcataaat
//
LOCUS       DAZFYC010000197         6014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFYC010000197 DAZFYC010000000
VERSION     DAZFYC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.199"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..607)
                     /locus_tag="ACHKW5_004890"
     CDS             complement(<1..607)
                     /locus_tag="ACHKW5_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO7514097.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCE"
     gene            complement(620..2722)
                     /locus_tag="ACHKW5_004891"
     CDS             complement(620..2722)
                     /locus_tag="ACHKW5_004891"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO7514098.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTHETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(2743..3369)
                     /locus_tag="ACHKW5_004892"
     CDS             complement(2743..3369)
                     /locus_tag="ACHKW5_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO7514099.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGALVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(3825..4034)
                     /gene="fumD"
                     /locus_tag="ACHKW5_004893"
     CDS             complement(3825..4034)
                     /gene="fumD"
                     /locus_tag="ACHKW5_004893"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO7514100.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            4389..4582
                     /locus_tag="ACHKW5_004894"
                     /pseudo
     CDS             4389..4582
                     /locus_tag="ACHKW5_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4590..6002
                     /gene="pykF"
                     /locus_tag="ACHKW5_004895"
     CDS             4590..6002
                     /gene="pykF"
                     /locus_tag="ACHKW5_004895"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO7514101.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMTSRLDFNNDSRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAQKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         1589 a         1517 c         1301 g         1607 t
ORIGIN      
        1 attcgcataa gccataagcc ttgccatagg tcataaatcg ctcgctaaac aagtacaaca
       61 ccgccgtcga tgactccacc tgccgtatat cggcatattc aggtgcaaca tccatcgcgg
      121 cgatcgcggc ttcaatctgc gcttcctgga agtaatatgg cgcttgcatc agcatggcga
      181 ctttgtaagg acgaggatac gtctggcact caaaacgaac ggcgtgtgca atggcgcgac
      241 aaatgtcctg ctccacaact tgcaaagaca tcgccgcgta gttttcactc atggcctgcg
      301 ttgagtaata gtaatcatcc tgacttcccg tcatacaagc gatatccgcg tagtctccgt
      361 tctgcttaat gctttccagc aaaactgaca gttcctcttc tgcaaccgaa tagggtggcg
      421 agagaaacac ggcgcgcgcc accagctgcc cagccacaga atgctggcga atgaactctg
      481 ccaggatttc agccggttct ggttcggtta ccgtatcaac ggggtgacgc atcccctgta
      541 accattgccg cttcgcctcg tcaacttcgc tgactttcgc taacggcaat tcatcccgat
      601 ggttcatctc acccattcct tacgcaggca gtagattgtg tgctgccaga tcggcggcaa
      661 tatcttccag ccccagacgc tgcaatgttt cgtgggtcgg acaaccaagc tgtgggtccc
      721 agcccatttc tttgtagaac atcgtgagcg aagcatgcat atcgtcacga tccattttgt
      781 cagtaccttc agtaaagacc gggatctgcg gatccttgtc gaatacccag gaacagataa
      841 gatcgtgttc attacgcata tctttggttt gcatcagttt taccgtgtag gcacgatgca
      901 acgtaaagat acgctctgca gctaaatcta atttttcctg agtcatctct tcgccggtga
      961 tcgctttgaa gaatttggct tcaagcgcca gatcgccccg gtaattacgg cttttcagcg
     1021 gcgaaacggt cattggccag acccagttgc acagggtgac ggcgttatgc aaacagaccc
     1081 gcaacagcga ccactttgca tatttaattt ttgcgtcatt gattggcgtg tagtttttgg
     1141 tttcatcgta ggcatcttca gaaccaaaaa gctctttcgc cacttcacgc tgcagtttca
     1201 atggcaaacc cgaaccaata aaattgatat gggtatgcgt catgcagtca cggttgaaca
     1261 tacagttaac aatggaaccg acctgcgctg acgcttcatt ggcatggtga accggatagc
     1321 caaacggcga ccagagttta ttttttgcgt agccccagta ctcttcaccc aaattccagc
     1381 gttctgcgat ggcatatgaa ccatcagcca ggtgactcag ctcacccaca cgatgcgcca
     1441 gacggtagta aaaatcttta atgaaattaa cgtcacccgc ttccagttga tcccagtgaa
     1501 tttccgcata ctcttcagct ggcagaacac gcttgaacac ccctttgctg taacaataag
     1561 taaagtcgcg atgcagctgc ccgtagttac accataggcc gtagtcatcg aacaaattca
     1621 gaccaaccag gttaccaatc acacgaccat cgtctttatc ttcaaaatct ttcggcccgt
     1681 tcgggaagat ggtggtatgg acaaagttgg ctacacaagt gttaccacct gtgctgggca
     1741 cgccaaactc tttcacccga ggaatattca tttgggtcat acaacggatc gggcaagagt
     1801 ggcagccact catttttact gtgtatttct ctgccgccgg acctaagtca aaaacggatt
     1861 tataggtacg aaagccgacc gtgttctgat tgcctggcgg aatttccccc gtttcaatcg
     1921 gaccaccttc agccgcgccc caaaacagcc ctttacgtgc tgtccaacgt gacttgggat
     1981 ctgaatactc tgcccacgat tgcggcgtac ttggcacgac atggttgtta ttcgcaccaa
     2041 taagttcagt catcatgtaa tcgttgagac gcttcatctc ctgacgatcg gcaatgttga
     2101 cccctttcgt cccttcaacc gcaatcgctt tcaggttttt cgaacccata attgcgccag
     2161 ttcccgcacc gccgctgtgg ttacggctat tcaacatgcc agagagagga acaaggtttt
     2221 ccccagcctg accaatagcc gccacacagg tttccggact ggtcaatcga caaatttctt
     2281 ccgtcgttgc gcgcgtccct tttccccata agaaatcggc tttttccagg ctaactttgt
     2341 catctttaat cttcagccat accggtgatt tcgccttccc ttcgataata atgacgtcgt
     2401 atccagcgaa tttcatttgc gctgcaaaaa agccacccat atgggcatcg acaactaaat
     2461 ttcctttggt aaaagtagaa agtgaggtga tatttacgcg agaactacag ggggcaccag
     2521 atccagttaa tgggccggta gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc
     2581 ctggcggtac ttcgtcatac ataattttgt agccgaagcc catgccaccg acaaaacttt
     2641 taaacttact ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat
     2701 tacctgtcca accgttagcc atgatttttt cctttgcaag attcacacag taatatcttt
     2761 ccactcgata atttttaacg cccctgttgg gcaggcgttt gcgcattcac cgcataacac
     2821 acatttcgag gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt
     2881 agtacaggcg ctacagccaa tacagcgttt atgatcgacg gtaatacagc cttctttctg
     2941 ctgccaggta atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg
     3001 gcaggtgtcc gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt
     3061 gtcgccaaag aaataattgc ggtggatttt gatacgggag aagaatgtcc ctactgagcc
     3121 atcgttgaag ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg
     3181 ggtcaccagc gcccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc
     3241 ctgtttgcaa cccaaaagtg acagtaacgc aggggcaatt gttagaccag caaggccttt
     3301 tcctgatata cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac
     3361 cgggttcatt tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat
     3421 attaagcatt caactaatac gctgctgact ctgtccccaa caccagatat tttccctaac
     3481 ccgatagtgt atgagaaaag atttaacgcc gataaactaa tcctttatgg ttaggagaaa
     3541 aaataatgtg attattctga gcccttaaca ttgatcgtta tcaattaaaa ttacaaagca
     3601 agaaattata agtgaactga tatttattat catttgaaat aaatttaact taagcgacaa
     3661 ttatttgtga cgttcattgc agaaaataaa aataacatta tcgctataaa ttaaaataca
     3721 taccgaaatt cgtataattt cagcagttgt tatttagaat gattaactgg accgcaactg
     3781 aagcaagaat atcaggatga agcttaccgc ctcatcctga atggtcattt taccagtgcg
     3841 ttaatgacgg tttccatcgc ctgcttttcc agctcactgc gctgaatgcg tttattggct
     3901 aatgctgtac gtaatacgcc tgcgataaca atatgctttg gttcctgccc cagatcgcgc
     3961 atttccagca caactttacc gacaactctg cacatttctc ggtacagttc atcctctttt
     4021 gtccgatttc ccattcttta tacctattta tcatgccaac tatcagcata tatcaatcta
     4081 accaattaaa caaaaatcaa acaaaatcag acaaataacg cgataaatta ttttaactgt
     4141 agcaattgag cgatgatata tttatacacc ggatgaactt tcacttatcc tcacactgac
     4201 aacttcggca ccagacgttg cgcaaacagt gaagtttttg cgtaaccttt tccctggaac
     4261 gttaaatctt tgataacaat ttattgtcta acaagttgta tattttttga aacgctgttt
     4321 ttgttttcct tttggattaa tttcagcgta taatgcgcgc caattgactc ttgaatggtt
     4381 tcagcacttt ggactgtaga actcaacgac tcaaaacagg cactcacgtt gggctgagac
     4441 acaagcacac attcctctgc acgctttttc gatgtcacct atccttagag cgaggcacca
     4501 ccactttcgt aataccggat tcgctttccg gcagtgcgcc cagaaagcaa gtttctccca
     4561 tccttctcaa cttaaagact aagactgtca tgaaaaagac caaaattgtt tgcaccatcg
     4621 gaccgaaaac cgaatctgaa gagatgttag ctaaaatgct ggacgctggc atgaacgtta
     4681 tgcgtctgaa cttctctcat ggtgactatg cagaacacgg tcagcgcatt cagaatctgc
     4741 gcaacgtgat gagcaaaact ggtaaaaccg ccgctatcct gcttgatacc aaaggtccgg
     4801 aaatccgcac catgaaactg gaaggcggta acgacgtttc tctgaaagct ggtcagacct
     4861 ttactttcac cactgataaa tctgttatcg gcaacagcga aatggttgcg gtaacgtatg
     4921 aaggtttcac tactgacctg tctgttggca acaccgtact ggttgacgat ggtctgatcg
     4981 gtatggaagt taccgccatt gaaggtaaca aagttatctg taaagtgctg aacaacggtg
     5041 acctgggcga aaacaaaggt gtgaacctgc ctggcgtttc cattgctctg ccagcactgg
     5101 ctgaaaaaga caaacaggac ctgatctttg gttgcgaaca aggcgtagac tttgttgctg
     5161 cttcctttat tcgtaagcgt tctgacgtta tcgaaatccg tgagcacctg aaagcgcacg
     5221 gcggcgaaaa catccacatc atctccaaaa tcgaaaacca ggaaggcctc aacaacttcg
     5281 acgaaatcct cgaagcgtct gacggtatca tggttgcgcg tggcgacctg ggtgttgaaa
     5341 tcccggttga agaagttatc ttcgctcaga agatgatgat cgaaaaatgt atccgtgcac
     5401 gtaaagtcgt tattactgcg actcaaatgc tggattccat gatcaaaaac ccacgtccaa
     5461 cccgcgcaga agcaggtgac gttgctaacg ccattctgga tggtactgac gcagttatgc
     5521 tgtctggtga atccgcaaaa ggtaagtatc cgctggaagc tgtttctatt atggcgacca
     5581 tttgcgaacg tactgaccgt gtaatgacca gccgtctgga tttcaacaac gacagccgta
     5641 aactgcgtat cactgaagca gtttgccgtg gtgcagttga aactgcagaa aaactggatg
     5701 ctccgctgat cgttgttgcg acccagggcg gtaaatctgc acgtgcggtg cgtaaatact
     5761 tcccggatgc aaccatcctg gcgctgacca ccaacgaaaa aactgcacat cagctggtgc
     5821 tgagcaaagg cgttgtgccg cagttggtga aagaaatcac ctctactgat gacttctacc
     5881 gtctgggtaa agaactagct ttgcagagcg gtctggctca gaaaggtgat gttgtggtca
     5941 tggtttctgg cgcactggta ccgagcggca ctactaacac cgcatctgtt cacgtcctgt
     6001 aatattgctt ttgt
//
LOCUS       DAZFYC010000198         5975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFYC010000198 DAZFYC010000000
VERSION     DAZFYC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.200"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(108..869)
                     /gene="kduD"
                     /locus_tag="ACHKW5_004896"
     CDS             complement(108..869)
                     /gene="kduD"
                     /locus_tag="ACHKW5_004896"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO7514102.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(899..1735)
                     /gene="kduI"
                     /locus_tag="ACHKW5_004897"
     CDS             complement(899..1735)
                     /gene="kduI"
                     /locus_tag="ACHKW5_004897"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO7514103.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2022..3203)
                     /gene="yqeF"
                     /locus_tag="ACHKW5_004898"
     CDS             complement(2022..3203)
                     /gene="yqeF"
                     /locus_tag="ACHKW5_004898"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO7514104.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3458..4687
                     /gene="yqeG"
                     /locus_tag="ACHKW5_004899"
     CDS             3458..4687
                     /gene="yqeG"
                     /locus_tag="ACHKW5_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7514105.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5147..5779
                     /gene="yqeH"
                     /locus_tag="ACHKW5_004900"
     CDS             5147..5779
                     /gene="yqeH"
                     /locus_tag="ACHKW5_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7514106.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         1659 a         1426 c         1257 g         1633 t
ORIGIN      
        1 attgataatt aatatggatt atttcataac catgatatgg attatgatga tctacaggta
       61 taaaaaaccc tgccatgcgg cagggtcata aaagtaagaa gaatgaatta acgcgccagc
      121 caaccgccat ccacggcaat ggtataacca ttcacataat ctgaagcgct ggaggcaagg
      181 aacactatcg gccccatcag gtcactcggc agtccccaac gaccagctgg aatgcggtcg
      241 agaatttccg cgctacgttg ttcatctgcc cgtagttgtt gagtattgtt ggtcgccatg
      301 taacccggag ctatcgcatt aacattaatg ttgtgtttag cccattcgtt cgccatcaat
      361 cgcgtcacac ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc
      421 tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag
      481 tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg
      541 tcccagtctt tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc
      601 accaggatat caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca
      661 tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc cgtgacctgc
      721 tcgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc
      781 agccccaacg ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta
      841 ccttcgagag aaaatgcact taaaatcata acaatacctt attcgtttat gcccacaact
      901 agcgcaaatc tttaacggcc acatggtcca tatcatcaaa gacctggttt tcaccgacca
      961 tgccccagat aaaggtataa gctttggttc cgacgccgga atgaatcgac cagctcgggg
     1021 agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca
     1081 tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct
     1141 cgtgagtgtg acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca
     1201 tactcaattg gcaggtttcc agcacatccg ggacaaaata tttgttaatt gtgcgacggt
     1261 tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg
     1321 tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa ctttgccgga gtggcggtat
     1381 caatactggc aaagacaact tcttttgcac ctttacccac atacagggcg tcgcggtgac
     1441 cgatttcata gcattggcca tcgacagtaa tcgtaccggc accgccaata ttgataacac
     1501 ctaactcgcg acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa
     1561 cggaaaccgt tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt
     1621 aaaccatggt gtactcatcg gcgacaaata cctcttcaac caaaaattca ttgcgcagcc
     1681 cttgggtatc cagcgttttt gcgtgcgcac tgtggatgct ctgtcttacg tccacatcaa
     1741 cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc
     1801 tctataaagg gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt
     1861 catttttaag ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg
     1921 gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat
     1981 taatgacggc agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg
     2041 gtcaatgcca cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta
     2101 cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg
     2161 tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc
     2221 ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca
     2281 gccaactgcc agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc
     2341 atcaatgccg gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa
     2401 ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg
     2461 gatgatgcat tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct
     2521 aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga
     2581 ccgttacttt ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc
     2641 gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca
     2701 tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac
     2761 aacccatcat gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg
     2821 ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata
     2881 tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgctttaag cccagaaccg
     2941 caaacgtcat taatagtgat tgcagaaacg ctattaggaa gaccaccttt aatagccgat
     3001 tgccttgccg gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc
     3061 accgcatatg caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt
     3121 tccacggcgg aatgacccgc taacgcaccg cgaaagcagc cgataggtgt ccgtaacgcc
     3181 ccgacaatca caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg
     3241 attgttaatt acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata
     3301 tgcctgagca gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca
     3361 atagaggggt agatagggcg ttaatctccc atacttaacc tggtttatgg taaattgccc
     3421 tccattttgt ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga
     3481 agaaactctg tggagtttcg cgctctacgg cacagccgtt ggtgcaggca cgctcttcct
     3541 tcctattcag ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg
     3601 gcctttaaca tattggccac ataaagcctt atgccagttc atcctctcat cgaaaacatc
     3661 agcaggtgaa gggataacgg gcgcagtaac acactactat ggcaagaaga ttggtaatct
     3721 gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat
     3781 taccaactca cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat
     3841 gttggtgagt ctgggtgttg tattaatcct gaatctcatt tttctgatgg gacgccatgc
     3901 aaccattcgg gtaatgggtt ttttggtatt cccattgatt gcctatttct tatttctttc
     3961 catttacctt gtcggtagtt ggcaacctga tctattaacg acccaggtag agttcaatca
     4021 gaataccctt caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac
     4081 gcccattatt tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga
     4141 taaatgcaaa aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt
     4201 tgtctttagc tgcctgcttt ctattccacc ttcgtatatt gaagcggcta aagaagaagg
     4261 ggtcaccatt ttatcagcgc tttctatgct gccgaacgcc ccagcatggc tgtcaatttc
     4321 cgggattatt gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt
     4381 tattgaaggt gccacggagg tcgtcaaaac aacactacag caggttggtg taaagaaaag
     4441 tcgtgcattt aaccgcgcac tatcaattat gttggtatcg ctgatcacct tcattgtttg
     4501 ttgcattaac ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat
     4561 gatacttttc atcatgccta cgttgtcaac gtatctgatc ccggcgctta aaccctggcg
     4621 ttccatcgga aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt
     4681 tagctgatat ccattgtgaa tggttcaaaa ttgctacaat tttgaaccat tccagttttc
     4741 ataatgttca ttcgttatta ttccgcacta tcacttccct catcatatcc tagtattatc
     4801 agttagttaa acacacaaag agatctgaac atcttaaata agactattta agatgcataa
     4861 cttagattcg caagatatct tccggtcatt caaaaacaat ttgcgatata gatattctca
     4921 taccatgaga tagttatctg aaccaccctc aaccaggtaa gattaaatca gtgaaacata
     4981 atcatattta ataacgagaa atgcatttta aaatgtctta atcagaatta tagcaaatac
     5041 agattaatcg caatttttcc cagcaaaatt acaaaatcat ggtgtattga catcatcaat
     5101 caccaatgaa attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag
     5161 tcgttataaa agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga
     5221 gtcaccgcgt ttgcagtact gcgcaaactc gtgtttttgt atgtggcccg aagaaagttc
     5281 atattttaat cgtggtgtgg tagaaggtat cttaacaaaa aaccacaatg cgagattaag
     5341 cggatatatt tttgtcgatt tttcagtaag ttttttacgt ctattccttg aaaaagactg
     5401 gattgactat cttgcaagta ctgacatggg gattgtttta gtcagcgacc gcaatatgca
     5461 atcgttagct aattactggc gaaaacacaa ctcagcaata tctgctgtca tatataatga
     5521 tgatggtctt gatgttgcca atgaaaagat cagacaacta tttattggtc gttatttatc
     5581 atttaccaga ggtaacacat taactcagat ggaattcacc ataatggggt atatggtttc
     5641 tggttataat ccatatcaga ttgctgaagt tctggatatg gatatccgta gcatctacgc
     5701 gtacaagcaa cgaatcgaaa agagaatggg tggtaaaata aacgaattat ttattcgttc
     5761 acattcggtc caacattgat actcaacgac cagccagaat catactctgt ttatacgggc
     5821 atttattgaa atgtccgtat tttataaata ggccgtcccc tcaagagtaa acaccatagt
     5881 atttaaaacc atcacacgaa aaattcagaa gcattacgaa atatgaattt acaaacaata
     5941 gtggcataaa tgttaaccat gttaatttac gtaaa
//
LOCUS       DAZFYC010000199         5934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFYC010000199 DAZFYC010000000
VERSION     DAZFYC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.202"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2463)
                     /locus_tag="ACHKW5_004901"
     CDS             complement(<1..2463)
                     /locus_tag="ACHKW5_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514107.1"
                     /translation="MLQIVGALLLLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNKKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHL"
     gene            complement(2586..5708)
                     /gene="agn43"
                     /locus_tag="ACHKW5_004902"
                     /gene_synonym="flu"
     CDS             complement(2586..5708)
                     /gene="agn43"
                     /locus_tag="ACHKW5_004902"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO7514108.1"
                     /translation="MKRHLNTSYRLVWNHITGAFVVASELARARGKRGGVAVALSLAV
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKADGTIINQEGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETRINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYSLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFHARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     SSQATLNVTF"
BASE COUNT         1257 a         1727 c         1548 g         1402 t
ORIGIN      
        1 gagatgggat gcatcatatt ccatgacggg gcgttgcctg atgatactga ccaccagtgg
       61 tttgattaac atattggtcg cggcccgttg ttgtataccg gcggcgaaaa tgatctccag
      121 agcattctga tcgataacac gggccagatt cgcgttcaga ttttcaatct catgtccggt
      181 cagcaggaaa ctgccggcaa gccatgtcac ctgctcctga atcattgcca gaattttgac
      241 gcgatcttct tcaaccagcg ctccggcaat ttttcctgat acagcggagg caatcattcc
      301 ccccagaaca ccgccaacca cacgccctgt cagtgcacca aatggtccca gtggactgaa
      361 caatatcccg cccgccactg acccgacagc accacctgcc acaccggaag atgccacggc
      421 aagattcctg atgaactgcg caccggagat ccgtccccgc aacattttga tcatgtcagg
      481 gccggttgtg acagctacca gtgccagaga tgtcaccagc gtacccttca tcactttgtt
      541 cagggcactg atatttcctg caccggtccc cttctgaagc gcctgccgga cagtcgggct
      601 ggccgtcgaa aaatcaatat gcttcagcat accctgaaca acactgagcc ggtgaagttg
      661 ctgggttgtg acgtagacag ccagtgtgcg ggtgaaggtt tttcctgcct ggacagcagc
      721 tgtctgcagg gcagcatcgc gatcgccggt gctgagccag aagaccgatg ccgtcagggc
      781 aaaactgatc cctccggccg ccagactgac aaccgacccc tcggcaatat cataagtgac
      841 cgattcgatg gtcccgaacc gggtgatatt acgggcctgg gtataagtca gatgtccccg
      901 acgaatcagc cgggacgctt ctgcgggatc ggttacaccg ggtactttac cttcgcggat
      961 cttattcttc atggtttcca cagcgccggc atactgatca cgggggactt ccagttgcat
     1021 gggaccatga tttcccatgt aacgatactg tccgttttgt ccgtcgaatg ccgcaccgac
     1081 gctacgggct gcagttgaac aatatttcgt ctggatttct gttccgctga ccagtctgtc
     1141 tgctccattt ctggcattat tatcaccgag gatccgtgcg tttttcagac ggactctgtc
     1201 gacaatatgg tttccacgtt cagcggcaaa cccatgtccc tgccctccgg aagcaaacat
     1261 tttctcattt gtactccagg tttccggcag tttcatgaga atcagaccat tcgccgttgc
     1321 atcggaaata aaaccggtgt aagaacgttc ataaatgtcc tgacacagtg tttctgtttc
     1381 gcgacaaaga gaggagcagt accattccgg atccggctta tgattttccg gggcatgatg
     1441 tacttctgtc agcccgcagg aggcacagat acgaagctca tccacgtggt ggaatgatac
     1501 aaaacggcgg ccttctgaaa aggggtacca gtcaccgccg tccaggcaac gatcgagaaa
     1561 atcgtcattc gcttccggtg ataaattcag gcggttccgg aagagggttt tcagttcatc
     1621 aggggtaaat gacaggctct ctctcagctt tttgttgaaa cccgccatgt ttctttcata
     1681 ccaggcattg tccagtagcc agtttccgtc tgccagttca aattccctca gttctccccg
     1741 ggaaatccgt tgtgaaatga gtgtcagcca gatgcgctcc gtcagcgttt caggaaggct
     1801 gtaatcgttt tgcaaactgc tcagccacga tttactgttg agcaccggtt gttcttccag
     1861 tgtctggcgg atggcactgg tcagtctttt cagcgcctgg atctggctgg ccgcccatag
     1921 cagcgtcact aatgcagcca gccagtacaa caacccgccc caccacatca gtgagggtga
     1981 aatatcgaac tgcaatgcga tcaatggtgc gtatgcagaa cacgccataa ggaaaaaagc
     2041 agtataaaaa cagcagtaat acccgttaat ttccttgctg atatcttcag gtgtgagccc
     2101 gttctgctga atgcttctta tctgcacggg aagagccagc agaccagcgg cgagagcggt
     2161 ggtcagaata aattttacag agtcaaaagt atgagcctcc agtgcgatat gtttaccatc
     2221 aagtccccac atgaagctga tgatcattcc ggcaatcgtt agaaatacgc ctgccagcca
     2281 gcgaatatgg aataaccggg ttatgcgttc agtgatatag ccagccagta aagccgggcc
     2341 gaacagacac agcaatacga atcctgccag cgcagacgcc gtgctggtta atgctctgaa
     2401 caaaaggcga agaatggcaa atcctgcgat cagcaggagc agcgcgccga ctatctgtaa
     2461 catagtcact ccgtgatgaa tatcatgtta ttgttaatgc cagcgaatgt ggcgttgaat
     2521 atccgttgcg ccggtccggg tcccgatcat gatgaccggg accacagaga ggcaatgcag
     2581 aattatcaga aggtcacatt cagcgtggcc tgactgctat aaccttcagc gctgctgccg
     2641 ttgatgctgt gggcataacc ggcctgaacg cccagggtga tattttcccg gacacgggct
     2701 tccagtccgg cctgcagatc cagtgaagtg ccattctgtg agggagagaa cgtcatcccg
     2761 ctgcctgcgg tggaggtccc cacacgcata tcaccccggg agctgaaggt gcggataacg
     2821 gaaggctgta cccaccagtt caccggcagt tcacgcacac tgtgttttgc actgtcacgc
     2881 aggggggcac gggatgaggt gccttcacca aaggtcatat cgttgtggct gcccagacgg
     2941 aaaccggcac gcacatgctg tgcactgcca tgcccgaact tcacataacc ggcgttgtcc
     3001 ttaccgtcat ccagcgaaag cccctgccag gtgtactgca gccgtggctc cagcatcagg
     3061 ttatcagtga tactgaaggg cagaccggtt tccagtgagc ccagccagcc ccggccccgt
     3121 gcgtggaagt cgttattgcc cgatgacgct ttcatactgt ggcgggttcc ctgtgccaca
     3181 atgtcagccc acaggccgga ggaggtgtgt gtcaggttca tatatccgcc caggctgccg
     3241 gcatcatccc ggaccgtgcc ggcgcgggag ccgtcatcat ccttaacatc aacggaagaa
     3301 tggcctgcag caccatatac ccctgcggtc acagacatac cggcaacctc tgttctcagc
     3361 aggtcaccct ccaggcggac gaagccatag ctgccgctgc tttccggcgt ggccccacgg
     3421 gcaataccgc cgttgttatc gtgaccgaga tggccaccct gaatgctgag acggacgctg
     3481 ttattttcac cgtttacacc ggtctgatgg ctgcgggagc ctgccagaat ccggtcatag
     3541 tccattgcct gtgtcagcat ggaggcgtac agggggactt cagcacgata acgttcttca
     3601 ctgcgcagat accagctctc atcactgtcc cggttgaggg agtagttaaa ggccccggcc
     3661 tgcagcatat tcccctggac aaaggcccct tcctccgtgg tggcaccgtt aatggcctca
     3721 accacctgaa tacccttacc ggtggtcgcc agccccgtac cactgttgcc ggcgttcacc
     3781 aggttaagga tggtttttcc ggttgccctg ccaccgtcaa tgaccagtct gtcagcattg
     3841 ttctgtgcca tatccgggcg tacacgcagg ctgatggtgc cattctgccc gttcaggttt
     3901 ttcacctgca gggttgtcgg aacgaacttc cctgtgcggg cggaggtgaa atgaatctgt
     3961 ccggcatggc tgaggtcatc caccaccgac tgaaccgtgg cgttatcggg gatattccag
     4021 gtggcaccgg atgtcagggt gacattcgtg gggtcaatgg caccgttcag cactgtgctg
     4081 ccggtcagct tcagggccgt gccgcgctga gcgatgacat ccgtggtgac ggtactgtcg
     4141 ttcagcgtca gcgtgccttc attcaggttg acggttttct gggtgagtgt ggtgttgctg
     4201 acagtgagtg tgccacttcc tgatttttcc accctgccgt taccggtaag agcgcctccc
     4261 tgcaggagtg catcaccttc acggatggtc agggtatcgt tattcaccgt tttcccggaa
     4321 agggtaagta tggcgccgtt attcagcgtg gtggtgcccg ccagtgtgcc gcccctggcg
     4381 gtgaacagtc cgccatttac cgtggtatcc gtggccgtat caccggcgtt cagcgtgaat
     4441 gaactgcctt tttccagcat cagggcatcc gcctgaccgc ctccgatacg gaatgccttt
     4501 ccgtcgctcc gggtaccact gacagcggca ccggaatcgg ccagcagtac accgccattc
     4561 cccatggata cggtggtggc ggcaccaccg ttgcggacat ccagcgttcc gccatcatcc
     4621 acacgggtat tagtggctgt gtgtccgctc agcacatcca gacgtccgcc attttccagt
     4681 acgacattat cagccttacc cgccacaacg gagaatgctc ccaggcggtt tgtgccggtg
     4741 acggttgcag ctgtactggt aaccagtgca ccgcccgtgt tctgggtgac atcagacgct
     4801 ttaccgccgg cattcacctg cagctttcct ttctggttga tggtggtatg cgcggcagtt
     4861 cctccttcct taatcacctg ccagccatca cggtttatcc gcgtttctgt tgccgtgcca
     4921 ccgttgtgta catactggtt acccccctcc agccgggtgt tatgtgcctc tccgtgtacc
     4981 gtctggtcac caccggcgta aatgagggtg tcccgtgcca tccccgaaga ccagataacc
     5041 tgccggccat ttttgttgat ggtggtggat tcagccgtcc ctccgaccat ctgaccgctg
     5101 gacacatttt cagactccgg accaccttct gcaccggtgt tgacgatggt tccggttgcc
     5161 agtccgccat gttttatggt ctggtaaccc tcctggttaa taattgtacc gtcagctttc
     5221 cctcccccgt gtacccactg ctcacctctg ttatccagcg tggtgttcac cgccagtccg
     5281 ttaaggctct gccctccgcc atcacgaata accgtatcac tggcagttcc tcctgcctgc
     5341 acaatctgac gaccatttgc ggtgacagtg gtgtttctgc ctgtgccacc cgcttttatc
     5401 cattgcccgc cggtgttttc gtcactgtcc ggccccagct caagcccggt actgacagtc
     5461 acgccatcag ctgttccgga tacaaactgg ttgtcatggt ttaccagtgt tccgccattc
     5521 actgtttcac ccgggtgcac aacgatgtca gcagccagca ccgggagtga cgtgaccacg
     5581 gcaagagaca gtgcaaccgc cacaccgcca cgtttacccc gtgcgcgggc cagttcggag
     5641 gcaaccacga aagcgcccgt aatgtgattc cataccagcc tgtagctggt gttcagatgt
     5701 cgtttcatca gcttttcctt acagagggtg aataaaaaag ccggtaccca caaatgtgga
     5761 taccggcagg tacaaaacag catggtcaga taaagtggtt tttcccggac ataaatgctt
     5821 ccactctctc atccgccaga gaagtatcag tccattgtgt gcaattccgt tctgcatgat
     5881 gaagagattc agctgccttt ttgcaggcca ttaaccacta taaatgatcg atat
//
LOCUS       DAZFYC010000200         5886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFYC010000200 DAZFYC010000000
VERSION     DAZFYC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.203"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..288
                     /locus_tag="ACHKW5_004903"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHKW5_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKW5_004904"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKW5_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO7514109.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHKW5_004905"
     CDS             complement(965..1639)
                     /locus_tag="ACHKW5_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO7514110.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKW5_004906"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKW5_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO7514111.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3817)
                     /gene="ureC"
                     /locus_tag="ACHKW5_004907"
     CDS             complement(2114..3817)
                     /gene="ureC"
                     /locus_tag="ACHKW5_004907"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO7514112.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(3810..4130)
                     /locus_tag="ACHKW5_004908"
     CDS             complement(3810..4130)
                     /locus_tag="ACHKW5_004908"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO7514113.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHKW5_004909"
     CDS             complement(4139..4441)
                     /locus_tag="ACHKW5_004909"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO7514114.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..5230)
                     /locus_tag="ACHKW5_004910"
     CDS             complement(4451..5230)
                     /locus_tag="ACHKW5_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO7514115.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5611..5886
                     /locus_tag="ACHKW5_004911"
     CDS             5611..5886
                     /locus_tag="ACHKW5_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514116.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1321 a         1538 c         1588 g         1439 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccggct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctgg cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag ttcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5221 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     5281 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     5341 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     5401 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     5461 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     5521 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     5581 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     5641 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     5701 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     5761 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     5821 catatcgagg gggggcctcc agagatgaat ggaataataa tatttcctga tgatataata
     5881 ccttag
//
LOCUS       DAZFYC010000201         5612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFYC010000201 DAZFYC010000000
VERSION     DAZFYC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.205"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..571
                     /locus_tag="ACHKW5_004912"
                     /pseudo
     CDS             1..571
                     /locus_tag="ACHKW5_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
     gene            588..848
                     /locus_tag="ACHKW5_004913"
                     /pseudo
     CDS             588..848
                     /locus_tag="ACHKW5_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(990..2114)
                     /locus_tag="ACHKW5_004914"
     CDS             complement(990..2114)
                     /locus_tag="ACHKW5_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO7514117.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(2844..3041)
                     /locus_tag="ACHKW5_004915"
     CDS             complement(2844..3041)
                     /locus_tag="ACHKW5_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7514118.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(3107..3377)
                     /locus_tag="ACHKW5_004916"
                     /pseudo
     CDS             complement(3107..3377)
                     /locus_tag="ACHKW5_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3606..3878)
                     /locus_tag="ACHKW5_004917"
     CDS             complement(3606..3878)
                     /locus_tag="ACHKW5_004917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514119.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWEAIYRSTSHYGKNVLLLQLA"
     gene            4198..4392
                     /locus_tag="ACHKW5_004918"
     CDS             4198..4392
                     /locus_tag="ACHKW5_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514120.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            4735..4863
                     /locus_tag="ACHKW5_004919"
     CDS             4735..4863
                     /locus_tag="ACHKW5_004919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514121.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(5173..5508)
                     /locus_tag="ACHKW5_004920"
     CDS             complement(5173..5508)
                     /locus_tag="ACHKW5_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514122.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1641 a         1283 c         1202 g         1486 t
ORIGIN      
        1 tcacttacac cgcctctcag cccggtaagc accagaaaat cattgatatg gccatgaatg
       61 gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg cctcaacacg gttttacgtc
      121 acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata caaccgggca gtgatgtgat
      181 tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac gtcagcgctg
      241 gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct tcggtgaacg
      301 cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg tcgtggtatg
      361 gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc acgttatcag
      421 caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac atctggcaag
      481 gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca aggtcatcgg
      541 gcattatctg aacataaaac actatcagta agttggagtc attacctgag aataatacct
      601 cctgggtaag tgagcagata ctttcagcgc ctccgaaaga tgtaaatatt cgacttggag
      661 tcggttcttt agaaggtaca accgtctcac atgttcagcg attgcatcat tcgttaatcg
      721 cagctggttt ggaaagtaac ctcactgtct ataccggtgg tcacgattat gcctggtggc
      781 gcggagcaat tattgatgaa ttagcaaatt ataattgcag gaaggtatca aataataact
      841 ctgtgtaaaa tatcgtttta tcgtcagata actctttctg caatattatt atgggagact
      901 atctcattta tttagtgatg tccttcattt atgtgatgaa ggacattata ttactcataa
      961 catttctcgt tgaaaatttc agcaatacgc tacaattgtt tcccgttgtt ttttaatttt
     1021 ctcatcaaaa cctctgcaat ctctgcagga ctgggttgtt cagccatttc agcggctacc
     1081 tgatctgaac agaagcgtag tgaatcatca taaagaaggc gatttaccaa atcactggtc
     1141 agtccatgct tgtccggaat aatcccacac ccacgcttcg caacaatttc tgcattaaca
     1201 gagcgatctg caccttcgcc aaacacaatc tgtggtattc cactatacag cgctgtcagg
     1261 gtattacctg cgccaccgtg atgaataaat ccatcagctc cattaaggaa tacacccata
     1321 ggtatccagt caaccagacg tacatttgat ggtagttttc gtaaaccagt acgagcattt
     1381 attgcaagtt gcaaaatgat atcagcatca acttcatttg cagaatccat aacccatgaa
     1441 atcagctcca gaccatcaac cattggtttt acagtgccaa ggctgataag taaacgtttc
     1501 cgatcagaat ccctgtccca ccatgtttcc tttacagcac ctccgttata aggaatatat
     1561 ctcattgaga taaccggttc tccagcattt ttaagaatgc tcatacttgg gggagcaaca
     1621 tcaatccatg ctaaatcaca tagtggaccg ctgactccat gccgcctgta agcattgctt
     1681 aaagaacggt ttaacatctg aatatgggca gatgtatgcg cgaatccaac agccagcatc
     1741 actgaaggaa ttctatattt agcagcaacc aatgggcctg ccggatcaag agggggatag
     1801 actattaaat ctggcctcca ttttcctgca aaatcgatga ggttatctgc catttcatcg
     1861 ctaaaaaaag agaaatgacc aataatatta ctttttttcc tcaactcttc ctgatggcga
     1921 tatccagcct ctgaatctaa acctggaact gcatcaaaaa caaccagtcc agcttctgct
     1981 gctttattcg cgaatttgcc agcgctacta attactacat catgtccaat tacacgaaag
     2041 gcctgagcca gagaaatcaa tgggtataac aaaccgtaca gcggtgggcc aataaaaaga
     2101 atacgttttc gcattaaaaa agtccgtctt ctgcttacct gtttattttt ctatctctaa
     2161 gaaaacataa ttaatattat ttttatgtga gcttatgcac aaaaataacc aagtatatta
     2221 tcttctggta gagattcagg atacagtttg taggagcttt cttttttgaa caatgatttg
     2281 caggacagtt atgctaaact agcggaatca gtatcacaac aataaatgac tgatcatacc
     2341 tgaaatttga attaacaggc atcatgaatg tcaatagtct aagcggtgac tacttatatc
     2401 gcttgcataa atactaaaca ctgactagaa aagtattgga taaccccgtc actaaaagta
     2461 acgcttcggt tatttgctcg gaatgtatta acgccaagat tgttagccgt tacgccttat
     2521 atcactggcg ctgacaaatg tttcttttct aatttactga accggcgcag acacgcagga
     2581 aagcacgccg gcaccggtgt aatgccagtt ctaatcaata agaatggaat gacgaatcag
     2641 tcaggaaggg ataacaaccc gctgctgaac ggaaggtatc agcaggaaga tgaacgggaa
     2701 tatcgccagc accaagggca gggccgggtt ccacaatacc agcgctggca ttttgcacat
     2761 tgccagcgta ccggcattac aacacgtttc atgctgctgc cccccaggat tcctcgattc
     2821 gttgctacat ccactgctct acttcacttc cactgctcca cttcactttt gtaccagcgg
     2881 ctgctgcgcc ccagtttgat gggtttaggg aaatggcctt cactgatcaa cttgtaaaac
     2941 catttgtcgg tcataccggt gaaactggtg atatagttca tgtcgatcag ggaattatcg
     3001 gcacgcagaa atacgctgtc gtggcttgtt gtagtcaaca tggtcagaat gtcctgtgag
     3061 atttatgaag attgtgcgaa tgcgggaaat ctactgagct gtgctttcag aactggcctg
     3121 ttacgggata ccagggatta ccggcggggt aacgggcttc cggatcatac acaccacgat
     3181 tatcgcggac aaaatcactg aacgcccata tcacctcttt aagtatgtct tcgcagtccg
     3241 gtaaatgacg atccagcgcc acatcccggg tggtacagct ttgatgcgcc cggtgacaca
     3301 aagcccggat tgttccagac atcctgaatc aaacgcccca gattaggggc gtcgaaatat
     3361 gcctctctga ccattatatt ccggcgtata ggtagcaggt ctgaagtgac aatgcgtcac
     3421 ctgacgttaa aagtcaccca agatgacccc agtgggggca acaagtataa aaccgttttt
     3481 tgattaagtg gtcggatatg aaaaggggaa ggtttatgtg aaaaatacct gtgttttctt
     3541 tgaaaacaca caacagtatc aggttattta tgtttataca gtgcgttcca aactgacctg
     3601 aataattacg caagttgcaa caataaaaca tttttgccgt agtgtgaggt actccggtaa
     3661 attgcttccc aggtgcagac aatgactaac gaagaactac aggcaatcgc attatccatg
     3721 cttgagcgcc agcgcgaaaa agtacaccag caacgagagt ctttgcttaa cccatctcca
     3781 ttcaccaggc caaaacgatt ggagcttgaa gcattcttgg acaaataccc acggcggctt
     3841 aaatcaggca aaaccagacc accaggctga tagctcacag tttgttctcc gattcaataa
     3901 aacggataag aaccatcagc gcggtagcgt aaggcactac agcgcattgc tttacgagta
     3961 ataacaatgg atttatcacc agaagacgca aagtttttac gagaactgca acaccgtatg
     4021 ctcagagaag ccattatcga acagcaaacg ggcatgagcg ccatagaccc attgcgatac
     4081 aaacgggcct catctccttt agttgacgac aattccaatg cggataaaac acgtccacca
     4141 cactgattac atttttcatt agcacagtgg tgtgatgcag catacgagga aaaccggatg
     4201 aatgagaaca aaataaaaag gctggagcaa ctgttacagg cacgccaacg ggagtttgcc
     4261 actaaatggg gacgtgccgc gctgccacat gaacgcctaa cctattttcc acttgaagac
     4321 ctcattaaaa ataatggcaa aaaaatcagg tctgataaga aacacttctt agaatgcaat
     4381 gaagacaaat aacaacaatc tgcatattgc caccaaccac agatgtggca tcgttatcag
     4441 cgcacttgat tttctttata tccactgacg agtttgccgg tcattctggt atcatgcaga
     4501 tatttacggc gtacaagcac actagcatga cattgtaata aggcctgagc ctaacgcttt
     4561 aatgtgtgtc atacactcag tggcgtaatc gttgacaata tggtgcatgt aatcgtaagc
     4621 gtccagcgaa atccatctgg tcataatctg aagcatccga caaagtggtg tccaccaaat
     4681 aagtgatctg cgcccaatat cctgagccgg aactttgaca aaggccaacg aaaaatggga
     4741 gcggatgtta cagagttctc ggtacagggg aaaagtggta cctgtcgcca gttctccgat
     4801 ctgtttaacc gggaaattat ctcctacagc ttgtcagaaa ggtcggtgat ggagcatcgt
     4861 taatacgatg ctatacgatg cattcacagt gcccggccag aggatgcccc gtcgctgcat
     4921 atggatcagg gttggcaata tcgaattgca ggctataggc aaagttaaag ccccatggag
     4981 tagcacaaaa tatgccgcac aaaggaaacg gtctgaataa cgcagtgatg aagaacttct
     5041 tcagcacact gctaaaacgc atagtgatcg agcgctgatt ctggcgaaca actgaactaa
     5101 tacatcagaa tctgcattat gttaaataaa tataaaaaga tggtttaaat accccgttac
     5161 ttttgactta cactatacgg tatcgcatcg tttaatattc gcaccggcca gatttttatt
     5221 tctattagtt gtcacaatac tgaatgcgta cgaccacagt attctggctc ctgtgtggtt
     5281 atgctttaat tctgcgttcc gggcagataa gcagttgctt gcaggaatcc ttcttgtgtt
     5341 aatgtcagtt ccccttttac cagtgctgat ttccacattc cgtccaacag agcttatagc
     5401 ctttccctgg attatagcat tgtccggctg aagttctttt tgaataataa tagaagcact
     5461 gctggcagat ccagtccgtt tttcataacc cactgtactg ataaccataa tctaatcagt
     5521 agaaattgag tcgaaaataa gcactactcc atacaggata attagaggtc agtttgatta
     5581 ttcacaattc atcatcagca ttttctattt ct
//
LOCUS       DAZFYC010000202         5586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFYC010000202 DAZFYC010000000
VERSION     DAZFYC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.206"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..444)
                     /gene="ridA"
                     /locus_tag="ACHKW5_004921"
     CDS             complement(58..444)
                     /gene="ridA"
                     /locus_tag="ACHKW5_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO7514123.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(517..978)
                     /gene="pyrI"
                     /locus_tag="ACHKW5_004922"
     CDS             complement(517..978)
                     /gene="pyrI"
                     /locus_tag="ACHKW5_004922"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO7514124.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(991..1926)
                     /gene="pyrB"
                     /locus_tag="ACHKW5_004923"
     CDS             complement(991..1926)
                     /gene="pyrB"
                     /locus_tag="ACHKW5_004923"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO7514125.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWFFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1930..2064)
                     /gene="pyrL"
                     /locus_tag="ACHKW5_004924"
     CDS             complement(1930..2064)
                     /gene="pyrL"
                     /locus_tag="ACHKW5_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO7514126.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2345..2740)
                     /locus_tag="ACHKW5_004925"
     CDS             complement(2345..2740)
                     /locus_tag="ACHKW5_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7514127.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(2871..3584)
                     /gene="bdcA"
                     /locus_tag="ACHKW5_004926"
     CDS             complement(2871..3584)
                     /gene="bdcA"
                     /locus_tag="ACHKW5_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7514128.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVIDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAIIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3655..4248
                     /locus_tag="ACHKW5_004927"
     CDS             3655..4248
                     /locus_tag="ACHKW5_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7514129.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEVAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            4393..4845
                     /gene="tabA"
                     /locus_tag="ACHKW5_004928"
     CDS             4393..4845
                     /gene="tabA"
                     /locus_tag="ACHKW5_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO7514130.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4968..>5586
                     /locus_tag="ACHKW5_004929"
     CDS             4968..>5586
                     /locus_tag="ACHKW5_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7514131.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCK"
BASE COUNT         1432 a         1303 c         1383 g         1468 t
ORIGIN      
        1 attttgcgtt tgtcatcaga cttaatccgg gcatgatagc ccggatttcc atcaagatta
       61 gcgacgaaca gcgatcgctt cgatctcaat ttttacgtct ttcggcagac gggcaacttc
      121 aacgcaagaa cgtgccggga aggtggcgtt gtgttcggtg aagaaggctt cgtaagtggc
      181 gtttacggtt gcgaagtcgt tcagatcttt cacaaacacg gtggttttaa cgatatcgcc
      241 cactttcagg ccagcggctt cgacaatcgc ttttacgtta tccagcgact gacgtgcctg
      301 tgcagcgacg tctgccggta cttcacccgt tttcggattt accgggatct gaccggaggt
      361 gatgatcata ttgcccagat caacgccctg tacgtaagga ccgatagctg ccggtgcatt
      421 ttccgtcgcg atagttttgc tcatgatttc tcctttatta cagcggtaaa aagtctggct
      481 cattataggg agccagactt ttattaccaa ccgcaattaa ttggccagca ccacattatg
      541 ggaaaactct ttttcacagt atttgcattt gagcgcgatg tcattggcgc gttttcgcac
      601 ggcaaagctg gaagaaaccg gttcggcatg gctgatacag ttgctgttcg ggcagaccag
      661 cacgttgtcg atgcgctccg gcagactggg gcgcgattta cccaccactt catagttgtc
      721 gatacggtta accgtggctt gcggtgcata caatgccagt tgatctacct gatcttcact
      781 caaaaaggta ttttcgattt tgatcagatc tttgcggccc atctcgccag aaggcaggtt
      841 cagaccaatg gtgatccgct gatccgtttc ggtcagcttg aacagactca acagcttaaa
      901 accgatctgg gcggggatat ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa
      961 tttattatcg tgtgtcatct ctatttcccc ttacagtacc agatcgcgat tcagaaccag
     1021 tgccagtaac gcctggcgag cgaaaatccc gttgcctgcc tgctggaaga accaggcgtg
     1081 tggcgtttta tcaacatccg tcgcaatctc atcaacacgc ggcagcggat gcagcacttt
     1141 catattggct ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt
     1201 ggcgtactcg gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca ggatgtctac
     1261 ttccgccatc acttcttcaa tagagctgtg cagactccat gcgatccctt tttcatcgag
     1321 catatccaga atgtattgcg gcattgccag cgcgtccggc gcgatgaagt aaaaacggtt
     1381 gccgtcgaac ttcgctaacg cctgagtcag ggagtgaacg gtgcggccat atttcaggtc
     1441 accaaccatt gcgacgtgga gattgtccag acgcccctgg gtttcctgaa tagtgaataa
     1501 gtccagcaag gtttgcgtcg gatgttggtt ggagccatca ccggcattca gtaccggtac
     1561 attgccggaa aactcggtgg ccaggcgcgc cgcaccttcc tgcggatgac gcatcactat
     1621 cgcatcgacg taagtgctga taaccgaaat ggtatcggcc agcgtttcgc cctttttacc
     1681 cagtgatgta ttggcgctgt cggagaagcc caccacgctg gcccccaggc ggtgcataga
     1741 tgtttcgaaa gagaggcggg tacgggtaga ggcttcgaag aaacagctgg caatgacttt
     1801 gtgcttcaac agctctggtt gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag
     1861 attaaggtca tcgcgactaa ggtcgtttat ggaaatgata tgtttctgat atagcggatt
     1921 agccatcttt tatctcctga cgcctgggca aaaaaaagcc cctcgattga ggggctggga
     1981 atgggtgatc aacgggaaga aaaacggcag gccagcgtct tttttcagac gcggtaagac
     2041 aaaatgtcga acacactgaa ccatacatcc tcccggcaaa ttgtccggca ttatactcat
     2101 cgtcagaagc ggcgcaagca tttgatgcaa tattttgtca gcgcaaacgg tttatttgaa
     2161 ttaaaagtca aggtatatgc atttttattt gatgtgattc tgcaggggaa ctgtccttcg
     2221 gtatcaataa ttgcagacat tccctgcttt ttcctttttc actttcacgc aatcagatat
     2281 gcattttatt ccattcatcc gacttatagg gcggagtgtg aaagcgaacg gctaacacta
     2341 ttgcttactg ctcagggatg cgcgctatca ctttaatttc aaaatcaaag cctgccagcc
     2401 atgtaacacc caccgccgtc cagtttggat aaggtggggc gctaaatatt tcatttttca
     2461 ccgtcatgat gtcttcaaat tggttttctg gatcggtatg gaagctcgta acatcaatga
     2521 tatcgtcaaa agtgcatccc gcagctgcca gggtcgcatg caaattatca aatgccagtc
     2581 tgacttgttg ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct acttgcccgg
     2641 aaacaaacag caaatcgccg gaacgaatag ccgcagaata acgatgctca gcatatagtg
     2701 aatgtcggcc agcagggaaa acagcggttc tttctaccat ttggttatcc tcaagattta
     2761 cgacatgaac agaagatttc tctttaccgg gagccgcttt tagcggacga cgtgagtaaa
     2821 caaaacccag acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca
     2881 aacgcgccat caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac
     2941 catgcgacca taccagcgac ctcttccggt tgcccatgtc ttttgatagc catcaaacta
     3001 tgcaacatat cgcgcattgg cccgttggcg ggattagcgt cggtatcaat tggccctggc
     3061 tggacgacgt taatggtgat cccacgcggt ccaaaatcac gggccagccc gcgcgccatg
     3121 ccttgcaggg cagatttgct ggcggcataa gcagccatgc ctgcaacagg catacgatcg
     3181 ccattcacgg agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca
     3241 acagaggcat gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca
     3301 tttaattcca gggcctcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat
     3361 gcgccgctct tacgaacgac atcaatgata gcgtctctgt cagcactatc tgtgaatact
     3421 gctgtcgctc cagtctcttg tgccaggcgt ttagcggcat ctttcgaccc cgcataggtg
     3481 aatcgtacat tggccccatc gatgacgaaa cgacgtacga tagcggcacc gataccacga
     3541 ctgccaccga ggatgagaac tgtcttacct gtaaaagcgc ccataaggac tccttgattt
     3601 attatgtaac atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc
     3661 actaaaaaac aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcaatc
     3721 tctgcggcaa aagtgctttt tcaccaaaaa ggtttcgatg ctgtcagtgt tgctgaagtt
     3781 actgattatc ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaagctggg
     3841 ttatttagcc gtgtactcaa tgaatacgtc ggtacggagg ctattccgct tgccgatatt
     3901 cttcgtgatg atcgtccagt aggcgagtgc ctggttgagg tattaaaaga agtggcgcgc
     3961 agatatagcc aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat
     4021 gatccactag cgcgtgatat tgccgttcaa tattatcacg ccgcagaaac gaccatttat
     4081 gactatatcg ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc
     4141 gtgatgtcag ggctttctgc gaaggcacgg gaggggcact caatcgaaca actctgtgca
     4201 acagctgcac tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga
     4261 tccgaaaggc gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa
     4321 ggctagcgga attgatcatc ttttcgtata acgatagaaa tgaaacgttg ttttaattaa
     4381 ggagtggaaa agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag
     4441 ttacgccagg cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag
     4501 cacgatatcg aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac
     4561 gaagcccgcc gtgcggagta ccatgcccgc tatctcgaca ttcagattgt gttaaaaggt
     4621 caggaaggca tgaccttcag cacgcaacct gcaggcacgc cggataccga ctggttagct
     4681 gataaagaca tcgcattttt gccggaaggc gttgatgaga aaacagttat ccttaatgaa
     4741 ggtgattttg ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggtgca
     4801 ccagcccagg ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta
     4861 aataactcca ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag
     4921 taaaatttgt aatgcttaga cgttcttatt aactcaagga gtccgtcatg agcaaaatat
     4981 caggttggaa tttttctcaa aacattacat cagccgacaa ttgtaaacaa aaaaatgaag
     5041 acttagatac ctggtatgtg ggaatgaatg attttgcccg aattgccgga gggcagaata
     5101 gcagaagcaa tattctttct cccagagcat ttttggagtt tttggctaag atatttaccc
     5161 tgggttatgt ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata
     5221 ttaagtcctc atcttatatc aaaaataatg atggtagtga gataatgaag tttgttatga
     5281 ataatcctga aggggagcga gcggatttat ctaaggtgga aatagaaatt acacttgcct
     5341 cagcttttaa taatgggatt cgtgaaggac atacagtcat tatatttaca caacctgatg
     5401 gttcgactaa tcgttatgaa gggaagtcct ttgaaagaaa agatgagagt tcattacacc
     5461 tgattactaa caagattctg gcgtgttacc aacgcgaagc taacaaggaa atagctcgtc
     5521 tattaaatat tccgcaggag ttaaataatt ctcaggactt gaataactcg caggtgagtt
     5581 gtaaag
//
LOCUS       DAZFYC010000203         5515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFYC010000203 DAZFYC010000000
VERSION     DAZFYC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.207"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            101..1198
                     /locus_tag="ACHKW5_004930"
     CDS             101..1198
                     /locus_tag="ACHKW5_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7514132.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1388..1771)
                     /gene="quuD"
                     /locus_tag="ACHKW5_004931"
     CDS             complement(1388..1771)
                     /gene="quuD"
                     /locus_tag="ACHKW5_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO7514133.1"
                     /translation="MCDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1789..2778)
                     /locus_tag="ACHKW5_004932"
     CDS             complement(1789..2778)
                     /locus_tag="ACHKW5_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514134.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2786..3595)
                     /locus_tag="ACHKW5_004933"
     CDS             complement(2786..3595)
                     /locus_tag="ACHKW5_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO7514135.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3615..4004)
                     /locus_tag="ACHKW5_004934"
     CDS             complement(3615..4004)
                     /locus_tag="ACHKW5_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7514136.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            complement(4001..4327)
                     /locus_tag="ACHKW5_004935"
     CDS             complement(4001..4327)
                     /locus_tag="ACHKW5_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO7514137.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(4324..4977)
                     /locus_tag="ACHKW5_004936"
     CDS             complement(4324..4977)
                     /locus_tag="ACHKW5_004936"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7514138.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTRKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(4977..5471)
                     /gene="yfdN"
                     /locus_tag="ACHKW5_004937"
     CDS             complement(4977..5471)
                     /gene="yfdN"
                     /locus_tag="ACHKW5_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7514139.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
BASE COUNT         1352 a         1523 c         1262 g         1378 t
ORIGIN      
        1 aaactaaaac ttacatcttg aaataatcac attgattaga tgaatattta tcgcgcagtg
       61 acatcatttt ttaataatag ttcaaaaaaa agggctcacg atgaaaaaat taacagtggc
      121 aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag ctgaaattta
      181 taataaagac agtaacaagc tggatctgta cgggaaagtt aatgccaagc actacttttc
      241 ctctaacgat gcagatgatg gtgatactac ttatgttcgt ctgggcttca aaggcgaaac
      301 ccaaatcaac gatcagctga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg
      361 cgctgaatct caaggttcct ccaaagacaa aacccgtctt gcatttgcag gcctgaaatt
      421 cggtgactac ggctcaatcg attacggccg taactacggt gtagcatacg acatcggtgc
      481 gtggactgac gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat
      541 gactggtcgc accactggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga
      601 tggtctgaac tttgctgctc agtaccaagg caaaaacgat cgtagcgatt tcgataacta
      661 caccgaaggt aacggtgatg gcttcggttt ctctgctacc tatgaatacg aaggattcgg
      721 tatcggtgca acttatgcga aatctgatcg taccgacact caagttaatg cagggaaagt
      781 tcttcctgaa gtatttgctt ccggtaaaaa tgcagaagtt tgggccgcag gtctgaaata
      841 tgacgctaac aacatttact tggccactac ctattctgaa acccagaata tgactgtatt
      901 tgctgatcac ttcgttgcta ataaagctca aaacttcgaa gctgttgcac aatatcagtt
      961 cgatttcggt ctgcgtccgt ccgttgctta cctgcaatct aaaggtaagg atcttggagt
     1021 atggggcgat caggacttag tcaaatatgt tgatgtaggt gcaacctatt acttcaacaa
     1081 aaatatgtct actttcgttg attacaaaat caacctgctt gacaaaaatg acttcactaa
     1141 agcactcggt gtaagcactg atgacatcgt tgctgtaggt ctggtttacc agttctaatc
     1201 tgattacgaa aaagatatgt tgcgggaggc gttgcctccc caacatataa gtggctccct
     1261 caagccactt cctttaggag cacaaccttg cttctaacta tataaacctt ctgttatata
     1321 ttacccttta tttttggggg cgttgcaacg ccccattttt aataactttc agtaaacaat
     1381 tggcatatta attagagtta ttaacaacga tatccatctc taaccggata tctaatgcca
     1441 ttaacatccc ttcaattatg ccctcagcct tctgtaacct tttcccgata taaccatcag
     1501 agcagcaatg cttacctgcc agtgacatga atgtcatacc gactacataa taatctacta
     1561 ataaatcgtg caaatcgctg ttgttctttt tcagacgggc catgcacccg caaatgatca
     1621 tcgcgtcatc gtcacaacat tgcgggcgag attttacttt tgaaggaatt aatcccttaa
     1681 aaccggcggc aatggacgac caggtcacat cttcatgatt attagccgcc cacgctcccc
     1741 aacgttcaag aaccatctga atatcacaca ttaactttct ccacaaaatc aggccagcac
     1801 accaatcgcc agtgcgcgat cgataaaacg aaatatcagc tccagctggg agccatactt
     1861 ctcttcaaat gccacggtat ccgcatgcag ttcgtcgtgg tgttttctgc acaaaggcag
     1921 cacaaaaagg tcatgcgctt ttgtacccat tccaccctga ccatggccta tcagatgatg
     1981 cggatcatca gctggctttc cacaacatgc acacggctgt gtcttaaccc agcgcgtgta
     2041 cttttcatta acccagcggc gacgttttgg gcgtaacata aaagactccg gcgactccgg
     2101 atccactttc agcgccagca cctttttcgc cttatcctgg atgatgctgg tggcaggaac
     2161 cgaaggcaca aggtcacttt cccgggtaac agacggcaca acaggcttcg gtaatctcag
     2221 tgccttacgg gctgcacttt ccggtaaggc atccgccaga tcattacgaa tcagccacca
     2281 gcacagttcc ggcattgtca caacgtgact gtcatcaaaa ccgagatccc gacgcacaac
     2341 agacaacacc cagcgggcac agttatccgt tgccattgat tccagccgtt ccgtgaactg
     2401 atcgcgcagc tggttatcgc agtgccagca cagacggatt gcacccggag cgtgtcgcat
     2461 tgtggtcatg ttctcgctgt gccagtcgga atgaggccac tggcagcctt tttcacgaag
     2521 taaccagctt tcaagacatt ccacgccacc agcacgacgg atcactgcct cattgcggaa
     2581 cacggcccga acggcaggat catccgccag cggttgtgat gccgccggaa cggcaccact
     2641 ggcgaaagat gaataacgct ccggctcagg ctccagcagg acacgcccct gcataaacag
     2701 gggcatcagc tctgaacctg gcctgaacaa tacgatcccc atacgcgggg caatttcagg
     2761 ggtcagtagt gctctcacgg tcacctcaat gaacggtatc gagcagcttt aacagctcag
     2821 ggaatcggga ttcgaagaaa tgcggctgcg tctcgcgcgg atttgcggga ctggtgatgt
     2881 tcttgccgaa catgcaacct ttcgctgtca gcgaccagaa ttttttgatg ttgttaatcg
     2941 ctgtacggct gtatcgttcg cgctgctcga cgatccccag tttcaccatc tggtgatatg
     3001 cctgattagc cgtcaggcgt ataccatact gtttcagcag tgcactcagt gacagtgtcg
     3061 ggcgacttga gccatcgtgt gcatcagcag gagcatcaat ggcatagcgc ggtgccagat
     3121 tcggtaagcc aacagcctcc tggagtttct gacaggcacc aagcactgaa gagttagaca
     3181 ggtttaactc ccggcgcata aagtccagca ggatcacgcc agcctgcatc ttgtcagcag
     3241 cctgtccgga taatttttcc ggtgcgctgg ttaccatatc gaaagtacgg atcaccttca
     3301 gatggaatga cgggctgatc cacattgcat aggcatacac cagttccttg cagacatacg
     3361 ttccccgttc atttccccca tgaatcacac tcaccgggtc aacacccaaa ttctgggtgt
     3421 tggtcaattc atgaacaagc tcaacagttt gttggctgga aagaaacttt cccggctcct
     3481 tggttctggc atttgcacca gatgctactg ctgcgcgatg cagatcgttc aggctgtaac
     3541 gcccataagc atcacgacga acttcaatac catcaatgac catcagatta ttcatacttc
     3601 gtttctcctc ttgatcaggc ggctgcaccc gccgttttct cgtacttact gatagtgatc
     3661 tcgaccttcc ctttcgggat aaccggtccc cactccacca gcattctttt cacctgactg
     3721 tcgtcttccc acacacccgc atgggtcagg gcgtcaaaca gcgccttgtt atagttgtcc
     3781 agatcgcgga tccggttatc cggaggaaac aacacgatct ccactgaagc aggtgccgac
     3841 gttggtttcg gcagacgacg taactgctca actattgctg cgcacgccgc gctctggaat
     3901 ttgcgccccg ctgcgcttat cagactctta ccagcaaacg cccctttgtt ggggtgtcgc
     3961 cagtacgtgt tcacgctggg agggaaaggc aagatcagct tcatactttc aggcccctct
     4021 catgtaacca gtgggctgca cgcagcctgg cgttttcctc accggcaagc agtgcgcgga
     4081 taatcccgac cgcctcgctg tcgtcgtcct tcaccgcggt atgaagagtt atcccccggg
     4141 ccacgccacg ctttatcgtg atgacgcctt ttttctccag tgcgcgaaga tgctccaccg
     4201 ctgcattcac cgaacggtat cccagcatgg ttgccacctc ctgattggtt ggcggaaagc
     4261 cacgttcttg ctggtaagaa atcagcatat ccagcacctg ctgctggcat tgagttaacg
     4321 tcgtcatgcc gccatctccc tgaccagttt ttccgcctgc tggcgaacct gcgccagaaa
     4381 ggcctcacca catgcctcaa gttcatcgcg cccgatgtag ctgattgccg gtcccttccg
     4441 ggtcttgtcg aaaacagcaa tagcaccagc gaagaaagcg cctgtcggca cctgcttctc
     4501 atccttcggg ataaaccagg caggcagttc aaaaccaata cgcccgcgaa taaaagcaat
     4561 atggtccgca tcttccggcc accacacttc gctggtggca gctttgatca ggaaaacata
     4621 gcgcccgccc ttatcacgca tggcactggc atgtttcatg atgtaacgca tgccggtgat
     4681 gtattgcccc tcatgctgac tggcgcggct gtatggggga ttaccaaagg cagcaccttt
     4741 aagctccgca agacgttctg accagtcatg cgccagcgcg ttatcttccg ccgtgtaata
     4801 cgcggcacat ttggcgttat caccgtcagt aaacagatcc agaacaaacg ggccaaacag
     4861 ggtgttaatt ccccagaaaa tgttgtccgg cgtgcgccac tgatcgccca cttccttcag
     4921 ttcatgggct ggtttgttcc gcagttccac cagcgcctgg caatatttat tactcattaa
     4981 gcccccacgt aattccctga cagataccac tcttcacccg atgcagcgcg cttgctgctt
     5041 ttccgtaagc accgctcacg acgcgccaga aaattgtttc gttctggctg ggagtggctt
     5101 tcacggaatg ccgccatcca caccgttgca gcacgacggt ataagcccct ggactccagt
     5161 tcttcagcct ggcgggtcag gcacaaaatc acccgtggat cgttagtgcc gacatagaaa
     5221 ttgcgcacag gtctggtttc tcgaactggt tgtggttccg gttcctgcgc tctctcagtc
     5281 aggcgcggga aatgtctgcg tgtatctcct tcacaacggt gagccacacg cccactctga
     5341 cgtaacttgc ttgctgactg cagaacgcgc tgccgtgagt aacctgcaaa agcatccgca
     5401 atgtctccgg aagtacatcc cggatgggct tcaatgaatt tctgaacttc attcaaaaga
     5461 ctcatgatca ccccctgaat cctgccggga tctggctgta gtccacgttg tcgta
//
LOCUS       DAZFYC010000204         5426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFYC010000204 DAZFYC010000000
VERSION     DAZFYC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.208"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            389..907
                     /locus_tag="ACHKW5_004938"
     CDS             389..907
                     /locus_tag="ACHKW5_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7514140.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1117..3258
                     /locus_tag="ACHKW5_004939"
     CDS             1117..3258
                     /locus_tag="ACHKW5_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7514141.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3334..>5426
                     /locus_tag="ACHKW5_004940"
     CDS             3334..>5426
                     /locus_tag="ACHKW5_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7514142.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPI
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGP
                     RSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLT
                     AETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKR
                     STIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTRKLT"
BASE COUNT         1278 a         1379 c         1693 g         1076 t
ORIGIN      
        1 aatctaatga ttgcttattt atatatttgt tttgtgattg attaagtggg tatttttaag
       61 gaatatctca aaaattatca aaatgataat tcagaaagga taaatacaat atttattcat
      121 gattcagctt tggccatttt atggaggcaa tacggacttt gaacagttgg gtatggttat
      181 ccagatatat attattaagt tattgctgaa taagagattt aatgatttta aaaatagttt
      241 ttacgacaac cgtaaattct agctcggttt gtatgtaaaa aatactgaaa aatgatgcgg
      301 ttccgtgaag tgagctgcat ctgcctgtct gggattaacg gcaggtcatt gtaagtagta
      361 attaaccacg ttaacaagga gtttacaaat gccaacaccg tgttatatct ccatcactgg
      421 tcagacccag ggaaatatta ctgcaggtgc atttaccgct gattctgtcg gcaacatcta
      481 tgtgcagggg cacgaagacg agatgctggt gcaggaattc ctgcataacg ttactgtccc
      541 gaccgatcct cagtccggtc agcctgcggg tcagcgtgct cacaagcctt tcatttttac
      601 cgtggcgctg aacaaagcgg ttccgctgat gtacaacgca ctggcctccg gcgaaatgct
      661 gccaaccact gaacttcact ggtggcgtac ctctgtggaa ggcaaacagg agcattattt
      721 cactacccgc ctgactgatt cgacgattgt cgatatgaaa ctgcatatgc cgcattgcca
      781 ggacccggcg aaacgcgagt tcacccagtt gctcgaagtt tcactggcct accgcaaaat
      841 tgaatgggaa cacgtcaaat ctggcacctc cggtgccgac gactggcgcg caccgctgga
      901 agcataagtt aagccaacag catcctgcta tcaggccgga tgctgttttt gtattacgcc
      961 gtagaaagaa aggttgtcag taaggggtta cgttcacggg agatggcggc tgccggaaac
     1021 ttcggcggta gcgtgcctga acgcaagcgt aacactgtac tgtcacagcc gctttaacag
     1081 cgaactatct gtttttcctt aaccccggag gttgctatgt caaccggatt acgtttcacg
     1141 ctggaagtgg acggcctgcc accggatgct tttgcggtgg tctcctttca tctgaaccag
     1201 tcactctctt cgcttttttc cctcgatctc tctctggtca gccagcagtt tctctccctt
     1261 gaattccagc agatcctcga caaaatggcc tacctgacga tatggcaggg cgatgacgta
     1321 cagcgccggg tgaaaggtat ggtgacctgg tttgaactgg gggagaacga caaaaaccag
     1381 atgctgtaca gcatgaaggt gtgcccaccg ctgtggcgca cagggctgcg ccagaacttc
     1441 cgtatcttcc agaatgagga catcgaaagc atcctcggca cgatattgca ggaaaacggg
     1501 gtgaccgagt ggagcccgct gttcagcgag ccacatcctt cccgtgagtt ttgtgtccag
     1561 tacggtgaga ctgattacga tttcctgtgc cggatggcgg cggaggaagg catcttcttt
     1621 tatgaggagc acgcgcaaaa aagtaccgac cagagcctgg tcctgtgcga taccgtgcgt
     1681 tatctgccgg agtcctttga gatcccctgg aatccgaaca cccgtaccga ggtgagcacc
     1741 ctctgcatca gccagtttcg ctacagcgca caaatccgcc cttcttccgt ggtgaccaaa
     1801 gactacacct ttaaacgacc cggctgggca gggcgttttg atcaggaagg ccagtaccag
     1861 gattaccagc gcacacagta tgaagtgtat gactaccccg gacgtttcaa gggtgcccac
     1921 gggcagaact ttgcccgctg gcagatggat ggctggcgca acaacgcaga agtggcgcgc
     1981 ggaacaagcc gttcgccgga gatatggccg ggacggcgaa ttgtgctgac ggggcatccg
     2041 caggcgaacc tgaaccggga atggcaggtg gtggcgagcg atcttcacgg cgaacagccg
     2101 caggcggtac cgggacgcag gggttcaggc accacgctgg ataaccactt tgcggtaatc
     2161 ccggcagacc ggacatggcg gccacagccg ttgctgaaac cgctggtgga cggcccgcag
     2221 agcgccgtcg tgacgggacc ggcaggcgag gaaatcttct gtgatgaaca tggccgcgtg
     2281 cgggtgaaat ttaactggga ccgctataac ccgtcaaacc aggaaagttc atgctggatc
     2341 cgtgtggcac aggcgtgggc aggcaccggc tttggtaacc tggcgatacc gcgtgtgggt
     2401 caggaggtca ttgtggactt cctcaacggc gatccggacc agccgatcat tatggggcgt
     2461 acctaccacc aggaaaaccg cacccccggc agcctgccgg ggacgaagac gcagatgacg
     2521 atccgctcga aaacctataa gggcagcggg tttaatgaac tgaagtttga cgatgcgaca
     2581 gggaaagaac aggtctacat ccacgcgcag aagaacatga acaccgaggt gctgaataac
     2641 cgcaccactg atgtgataaa caaccatgcc gaaaaaatag gtaacaacca ggcgatcacc
     2701 gttaccaata accagatcca gaacattggc gttaatcaga tacagacggt tggtgtcaac
     2761 caggtggaaa cggtgggcag taaccagatt atcaaagtgg gatcaaacca ggttgaaaag
     2821 gtggggatca ttcgtgcgct gacggtgggt gtggcttacc agacgacggt aggcggcatt
     2881 atgaatacct cggtggcgtt gttgcagtcc tcacaggtag ggctgcataa atcactgatg
     2941 gtgggaatgg gctacagcgt caatgtgggg aataacgtca ccttctcggt gggcaagaca
     3001 atgaaggaaa acaccggaca aacagcagtt tattctgccg gtgagcatct tgaactctgc
     3061 tgtggtaagg caaggctggt gctgacgaag gacggaagca tatttctcaa tggtacgcac
     3121 attcatctgg aaggggagtc ggatgtgaac ggtgatgcgc cagtgattaa ctggaactgt
     3181 ggtgccacac aacctgtacc ggatgcgcct gtgccgaaag atttaccccc cggaatgccg
     3241 gatatgcggc aattttgagt caggtgcaaa aaattaaaac ggaactggtg cagtgaccag
     3301 ataaaccata ttaaaagcag aaggaattga ttcatgggcg gaaaaccggc ggcgcggcag
     3361 ggtgatatga cccgcaaggg actggatatt gtgcagggtt cagcgggggt gctgataggt
     3421 gcgccgacgg gagtggcctg ttcggtatgt cccaacagcc cgatagagga gcagaagggt
     3481 aacccggtga acccgctgct gggagcaaag gtgctgccgg gcgagacgga ccttgcgctg
     3541 cccggcccgc tgccgtttat tctttcccgc gcctacagca gctaccggac cagaacaccc
     3601 gcgccggtgg gggtgtttgg tcccggctgg aaagcgccct tcgatatccg cttacagata
     3661 cgcgatgaag gcctgatact caacgacagc ggcggcagga gcattcactt tgagccgctg
     3721 tttcccggcg agataagcta cagccgcagc gagtcactct ggctggcgcg gggcggagta
     3781 gcggcgcagc acagttcgca gccgctaagc gcgctctggc aggtgctgcc ggaagatgtt
     3841 cgcctgagtc cgcatgtgta cctggcgaca aacagccttc aggggccgtg gtggatactc
     3901 agctggccgg agcgggtgcc gggggcggac gaggtgctgc cgccggagcc gcccgcatac
     3961 cgggtgctga cgggcgtggt ggacggcttc gggcggacgc tggcctttca ccgggcggcg
     4021 gagggtgatg tggcgggcgc ggtgacgggg gtgacggacg gcgcgggccg ccgttttcac
     4081 ctggtgctga ccacacaggc acagcgggcg gaagtattcc gtaaacagcg cgccacgtct
     4141 ttatcttccc ctgccggtcc ccgttctgct tcctcttctc tggttttccc cgacacgctg
     4201 cccgccggta caggatacgg cacggataac ggtatccggc tggaggcggt atggctgaca
     4261 cacgacccgg catacccgga tgaacagccg actgcgccgc tggcgcgcta cacgtacacg
     4321 gccggtggtg aactgcgggc ggtgtatgac cgcagcggaa cgcaggtgcg cgggtttact
     4381 tatgatgcgg agcacgccgg gcggatggtg gcgcaccatt atgcggggag gccggagagc
     4441 tgctaccggt atgatgatac cggtcgggtg acggagcagg tcaacccgga ggggctggac
     4501 taccgctttg agtacggaga gagccgggta atcatcacgg acagcctgaa ccggcgggag
     4561 gtgctgtaca cggaaggcga gggtggcctg aagcgggtgg tgaagaagga acatgcggac
     4621 gggagcatta cccgcagcga gtatgatgag gcggggaggc tgaaggcaca gacggatgcg
     4681 gcggggcggc ggacggagta cagcctgcat atggcgtcgg gtgcggtgac ggcggtgacg
     4741 gggccggacg gcaggacggt gcggtatggc tataacagcc agcggcaggt gacgtcagtg
     4801 acgtacccgg acgggcttcg cagcagccgg gagtatgatg agaggggaag gctgacggcg
     4861 gagacctcgc gcagcggtga gacaacgcgg tacagctatg atgacccggc gagtgagctg
     4921 ccgacaggga tacaggacgc gacgggcagt acaaaacaga tggcatggag ccgttacggt
     4981 caactgctgg ccttcacgga ctgctcgggg tacacgacgc ggtatgagta cgaccggtac
     5041 ggtcaacaaa tcgccgttca ccgggaggaa ggcatcagca cttacagcag ttataacccg
     5101 cgtgggcaac tggtcagtca gaaggatgcg caggggcgtg aaatccgtta tgagtacagc
     5161 gccgcaggcg acctgacagc aacggtatca ccggacggta aacgcagcac catcgaatat
     5221 gataaacgcg ggcgtccggt gagtgtgacg gaaggcggtc tgacgcgcag catggggtat
     5281 gacgctgccg ggcgcatcac tgtgctgacc aacgagaacg gcagccagtc cacgttccgg
     5341 tatgacccgg tcgacaggct gactgaacag cgcggttttg acggccggac gcaacgttac
     5401 cactatgacc tgaccagaaa actcac
//
LOCUS       DAZFYC010000205         5397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFYC010000205 DAZFYC010000000
VERSION     DAZFYC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.209"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(439..654)
                     /locus_tag="ACHKW5_004941"
                     /pseudo
     CDS             complement(439..654)
                     /locus_tag="ACHKW5_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1986..2168)
                     /locus_tag="ACHKW5_004942"
     CDS             complement(1986..2168)
                     /locus_tag="ACHKW5_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514143.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            2107..4197
                     /gene="iha"
                     /locus_tag="ACHKW5_004943"
     CDS             2107..4197
                     /gene="iha"
                     /locus_tag="ACHKW5_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO7514144.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQLTEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(4415..4565)
                     /locus_tag="ACHKW5_004944"
                     /pseudo
     CDS             complement(4415..4565)
                     /locus_tag="ACHKW5_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4777..5097)
                     /locus_tag="ACHKW5_004945"
                     /pseudo
     CDS             complement(4777..5097)
                     /locus_tag="ACHKW5_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
BASE COUNT         1548 a         1162 c         1246 g         1441 t
ORIGIN      
        1 tattctggga actatattct caaaatacag tagaaacgag gtatgtttct ggtggaaagg
       61 acagtgggat taaaaagtaa gaattgataa aaaaaacgcc agcaacacat agtgccggcg
      121 gagggaatac cccatggaga aaatgtgatg cctagagcat catgaatata taattaaaaa
      181 tagttagcgt tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta
      241 tgcatgacct acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca
      301 aacacaatat tcatagtatg gcgatgccta cgccagcaag aatagcgcca ataatattgg
      361 gaatataata gacaccagac gcacaggcat ctcactcctt aacaaacaac aatcaggata
      421 tttactttta ccaagccaac tgtttacacc caaagtacac acaattaacc attcaataac
      481 aaatggcaat atccatagcc atacgacttt accttgtaat gttcggtatt tctttataat
      541 tattctggga aatctaacat ttatttttaa aatcaaatta tcttgttgtt taaaaataag
      601 ttcacatact ttatcatctt cttccgccat caacatctct gcatacttaa acatttcaga
      661 acgttccttt agcacagaag agtaattata tatccagttc ccaggcaata atgcttatgg
      721 atatttaatt cataatttag agaatatttt gcaataatat ttggcagtat tcagaaatac
      781 ctgaaaaatc atactataca gccctaggga atggataaag attctaacaa agcatttcaa
      841 caatatatac ttgttaaaaa tccatatcga aatgccgttg cagcattaat atatgcctca
      901 ttcataaaat gtaaaagagc aagctcgtac cagtaggggg gaaattcatt gacatgtcct
      961 gtcaatacgt acagagccct gccatgcttg ccagcacgta acaattccgc ccccagtaac
     1021 cagcgtgttc cctgattatt ctcagggtta tatgttagta ttttatcaat gagtataacg
     1081 gcatcctgat gacgctgtaa atgaacattc gccagaattg cagcataaag agcgcaaatg
     1141 catatgccag atgagcgtga atatcaatga tgtcaggagc atgtcaggca agacgcctga
     1201 gtttgttgac gtaatttgtt tcgtttattt catcactatc gtatgcgtca agagtttttt
     1261 caaactcatc ccgaaatggg ccaagcctgg tttcaggaaa taagaaatac ccttggttat
     1321 tgttcactta aaagtataat ttgtaattca aatcctgagc ctgtaaagcg gggagtaaca
     1381 tattctattc tgaagagaat aaaagtcgtg atgcgatgta tcaagcccgg attgtaatcc
     1441 cagattaaca tagatcacaa aacaacttat tcttcactaa cgtcaagata aatatcgttg
     1501 tatgccttat cacgactacg aataccagca agatacaact gacatacgga aagataccac
     1561 gttttttact cccgaaaata acgctaaaaa gctacttccc catcgtttgt ccttagtatt
     1621 gccagcgcca acaatgtggg ctgacatgat aaagctgtct aggaaattgt tcgcctcctc
     1681 agcggacaat ccaaatggtg attgtctctg ttaaacgttt attttgaagg tcgactgaat
     1741 aaggtgatga cgctgtagaa tttttcacgt gccacagaat tttgaacgct ttctcttaca
     1801 atatttcaat gtttctatca gtattcgccg gaagaagtca tcgaccaaat catcccagtc
     1861 gtctcgcatc actgaccatt catggttgac atgtggtgga aatccgcttc tacagtaacc
     1921 attttttatt cgcaaaaccg aacacgccat acggatagct gttaactggc atgccgaggc
     1981 agtcgttatt tatatttagt tttgtcaata atctttattt tttgtaaaag gcaaatataa
     2041 attattctca ttattgtttg tatttgtgta ttgtcttgcc ggttaacatg atcggagatt
     2101 agtaatatgc gaataaccac tctggcttcc gtagtcattc cctgtctcgg attttcagcc
     2161 agcagcatag ctgctgcaga ggatgtgatg attgtctcgg catccggcta tgagaaaaag
     2221 ctgactaacg cagccgccag tgtttctgtg attagccagg aggaattgca gtccagccag
     2281 taccacgatc tggcggaggc tctgagatca gtagagggtg tggatgttga aagtggtacg
     2341 ggtaaaaccg gagggctgga aatcagcatc cgaggaatgc cagccagtta cacgctgata
     2401 ctgattgatg gtgttcgtca gggcggaagc agtgacgtga ctcccaacgg tttttctgcc
     2461 atgaataccg ggttcatgcc ccctctggcc gccattgagc gtattgaggt tatcaggggg
     2521 ccgatgtcca cactgtatgg ctctgatgcg atgggcggtg tggtgaatat cattaccaga
     2581 aagaatgcag acaaatggct ctcttccgtc aatgcagggc tgaatctgca ggaaagcaac
     2641 aaatggggta acagcagcca gtttaatttc tggagcagtg gtccccttgt ggatgattct
     2701 gtcagcctgc aggtacgcgg tagcacacaa cagcgtcagg gttcatcggt cacatcactg
     2761 agcgatacag cagccacgcg tattccttat cccacggagt cacagaatta taatcttggt
     2821 gcacgtcttg actggaaggc gtcggagcag gatgtgctct ggtttgatat ggataccacc
     2881 cggcagcgtt atgataaccg ggatgggcaa ctggggagtc tgacgggggg atatgaccgg
     2941 accctgcgct atgagcgaaa caaaatttca gctggctatg atcatacttt caccttcgga
     3001 acatggaaat cgtatctgaa ctggaacgag acagaaaata aaggtcgtga gcttgtacgc
     3061 agtgtactga agcgcgacaa atgggggctt gccggtcagc cgcgggagct taaggaatcg
     3121 aaccttatcc tgaattcatt actgcttacc cctctgggag aatctcatct ggttacggtg
     3181 gggggcgagt ttcagagctc gtccatgaaa gacggagttg tccttgccag cacaggtgaa
     3241 actttccggc agaaaagctg gtcggtattt gctgaggatg agtggcatct cacggatgca
     3301 cttgcgctga ctgcgggcag ccgctatgaa catcatgagc aattcggggg acacttcagt
     3361 ccgcgtgcat atctggtctg ggatgtggca gatgcctgga cgctgaaagg cggtgtgacc
     3421 acgggatata aggcacccag aatggggcag ctacataaag ggattagtgg tgtgtccggg
     3481 cagggaaaaa caaatctact tggtaacccc gacctgaagc cggaagagag cgtcagttat
     3541 gaggctgggg tgtattacga taaccccgcc ggtctgaatg ccaatgtcac aggttttatg
     3601 actgacttct ccaacaagat tgtctcttat tccataaatg ataacaccaa tagctatgta
     3661 aacagcggaa aggcccggtt gcacggtgtg gaatttgccg gcacattgcc gctgtggtca
     3721 gaggatgtca cgctgtcact gaattacacc tggacccgaa gtgaacaacg tgatggtgat
     3781 aacaaaggtg cgccgctgag ttatacccct gaacacatgg tgaatgcgaa actgaactgg
     3841 cagctcaccg aagaggtggc atcatggctg ggtgcccgtt atcgcgggaa aacaccacgt
     3901 ttcacccaga attattcgtc actgagcgct gtacagaaga aagtgtatga tgagaaagga
     3961 gaatacctga aagcctggac ggtggtggat gcaggtctgt cgtggaagat gacggatgcc
     4021 ctgacgctga atgctgcggt gaataacctg ctcaacaagg attacagtga cgtgagcctg
     4081 tacagtgccg gtaagagtac gctgtatgcc ggtgattact tccagacggg atcatcaaca
     4141 acaggatatg tgatacctga gcgaaattac tggatgtcgc tgaactatca gttctgataa
     4201 taacaaaacg ctatcactga cggtagaata cgttgccact gcaactcctg gcggaacagt
     4261 ggcaacgtat taggttaagt gcatttccga tccgctaatg agatttcgtt accaacaact
     4321 aatatcgtca caggaaatgc acggattatt tttaacttat catttacata cttgtccaga
     4381 gtgtaagcgc accgtgaagg acgtggggta aaaattagtt tacagagaga gtgacgttcc
     4441 agggcaacaa ctctttcatg cggttggcag gccaggtgtt gattacactg atcacgtggc
     4501 gtagccacgt ttccggctcg attccgttaa gttttgcagc taccgatcag gctgtacatc
     4561 actgccgcac tatcgctcgt catctcaaag tccggtctcg tcagcaggaa ggtatcattc
     4621 tctcccgcca tttttccagg ggcccggtca gataagtccc tttgtctatc gctgactcct
     4681 gactcataac ccggttagca gaatgcaggg tcaccacctc gccacgacca aatccaaata
     4741 agtcaattgc accttctcaa tcgccatttt gtcagtaagc gtacagcctg aactgatggt
     4801 atcttcacca tcaatgacaa cggtgatcgc aattttactg acgttcgccg gaacacgatc
     4861 cagtgctatc tcaatgctgg cctgctgcga accggtaacg agcctgactg ccccctcagg
     4921 agaagacaaa ttattataaa agataaagtc agaatcgcca ctgacctttc cctgagcatt
     4981 aagcatgaac aggcaggtat cgggttcgcc tttaaagccg gatttggcag ggtactgaag
     5041 attcagcctg atcgtagatt gctgaagggg tatgttttgt ccggattgta gattcatatt
     5101 aactctcctg atttttgatt attattaatg cgcagcgttt atatatgttc cctaggctta
     5161 gttctggacg ctggatattc ggtgaggcgt aaatatggta tgacaccatt tttcataacg
     5221 ctgaagtttc tatacctgtt gaattggaat tttcattgac caggtatctt attttccagg
     5281 gcccgttcct tcataagtcg caaaagtaac atatatccga agggcatgct gttgatatca
     5341 gacacggaat actggcttta accagcaacc acagataaac ccggggcctg cataaaa
//
LOCUS       DAZFYC010000206         5381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFYC010000206 DAZFYC010000000
VERSION     DAZFYC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.210"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            278..1612
                     /gene="cadB"
                     /locus_tag="ACHKW5_004946"
     CDS             278..1612
                     /gene="cadB"
                     /locus_tag="ACHKW5_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO7514145.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFNAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1692..3839
                     /gene="cadA"
                     /locus_tag="ACHKW5_004947"
     CDS             1692..3839
                     /gene="cadA"
                     /locus_tag="ACHKW5_004947"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO7514146.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3898..5355
                     /gene="dtpC"
                     /locus_tag="ACHKW5_004948"
     CDS             3898..5355
                     /gene="dtpC"
                     /locus_tag="ACHKW5_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO7514147.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         1272 a         1289 c         1308 g         1512 t
ORIGIN      
        1 tgccgcataa tttttatcac ataaatttaa ccagagaata tcccgcaatc cattgtaaac
       61 attgaatgtt tatcttttca tgatatcaac ttgcgatcct gatgtgttaa taaaaaacct
      121 caagttctca cttacagaaa cttttgtgtt atttcaccta atctttagga ttaatccttt
      181 tttcgtgagt attcttagcg ccagtttggt ctggtcagga aatagttata catcatgacc
      241 cggactccaa attcaaaaat gaaattagga gaagagcatg agttctgcca agaagatcgg
      301 gctatttgcc tgtaccggtg ttgttgccgg taatatgatg gggagcggta ttgcattatt
      361 acctgcgaac ctagcaagta tcggtggtat tgctatctgg ggttggatta tctctattat
      421 tggtgcaatg tcgctggcgt atgtatatgc ccgactggca acaaaaaacc cgcaacaagg
      481 tggcccaatt gcttatgccg gagaaatttc ccctgcattt ggttttcaga caggtgttct
      541 ttattaccat gctaactgga ttggtaacct ggcgattggt attaccgcgg tatcttatct
      601 ttccaccttc ttcccagtat taaatgatcc tgttccggcg ggtatcgcct gtattgctat
      661 cgtctgggta tttacctttg taaatatgct cggcggtact tgggtaagcc gtttaaccac
      721 tattggtctg gtgctggttc ttattccggt agtaatgacc gctattgttg gctggcattg
      781 gtttaatgcg gcaacttatg cagctaactg gaatactgcg gataccactg atggtcatgc
      841 gatcattaaa agtattctgc tctgcctgtg ggccttcgtg ggtgttgaat ccgcagcagt
      901 aagtactggt atggttaaaa acccgaaacg taccgttccg ctggcaacca tgctgggtac
      961 tggtttagca ggtattgttt acatcgctgc gactcaggtg ctttccggta tgtatccatc
     1021 ttctgtaatg gcagcttccg gtgctccgtt tgcaatcagt gcttcaacca tcctcggtaa
     1081 ctgggctgcg ccgctggttt ctgcattcac cgcctttgcg tgcctgactt ctctgggctc
     1141 ctggatgatg ttggtaggcc aggcaggtgt acgtgccgct aacgacggta acttcccgaa
     1201 agtttatggt gaagtcgaca gcaacggtat tccgaaaaaa ggtctgctgc tggctgcagt
     1261 gaaaatgact gccctgatga tcctcatcac tctgatgaac tctgccggtg gtaaagcatc
     1321 tgacctgttc ggtgaactga ccggtatcgc agtactgctg actatgctgc cgtacttcta
     1381 ctcttgcgtt gacctgattc gttttgaagg cgttaacatc cgcaactttg tcagcctgat
     1441 ctgctctgta ctgggttgtg tgttctgctt catcgcgctg atgggcgcaa gctccttcga
     1501 gctggcaggt accttcatcg tcagcctgat tatcctgatg ttctacgctc gcaaaatgca
     1561 cgagcgccag agccactcaa tggataacca caccgcgtct aacgcacatt aattaaaagt
     1621 attttccgag gctcctcctt tcattttgtc ccatgtgttg ggaggggcct tttttacctg
     1681 gagatatgac tatgaacgtt attgcaatat tgaatcacat gggggtttat tttaaagaag
     1741 aacccatccg tgaacttcat cgcgcgcttg aacgtctgaa cttccagatt gtttacccga
     1801 acgaccgtga cgacttatta aaactgatcg aaaacaatgc gcgtctgtgc ggcgttattt
     1861 ttgactggga taaatataat ctcgagctgt gcgaagaaat tagcaaaatg aacgagaacc
     1921 tgccgttgta cgcgttcgct aatacgtatt ccactctcga tgtaagcctg aatgacctgc
     1981 gtttacagat tagcttcttt gaatatgcgc tgggtgctgc tgaagatatt gctaataaga
     2041 tcaagcagac cactgacgaa tatatcaaca ctattctgcc tccgctgact aaagcactgt
     2101 ttaaatatgt tcgtgaaggt aaatatactt tctgtactcc tggtcacatg ggcggtactg
     2161 cattccagaa aagcccggta ggtagcctgt tctatgattt ctttggtccg aacaccatga
     2221 aatctgatat ttccatttca gtatctgaac tgggttctct gctggatcac agtggtccac
     2281 acaaagaagc agaacagtat atcgctcgcg tctttaacgc agaccgcagc tacatggtga
     2341 ccaacggtac ttccactgcg aacaaaattg ttggtatgta ctctgctcca gcaggcagca
     2401 ccattctgat tgaccgtaac tgccacaaat cgctgaccca cctgatgatg atgagcgatg
     2461 ttacgccaat ctatttccgc ccgacccgta acgcttacgg tattcttggt ggtatcccac
     2521 agagtgaatt ccagcacgct accattgcta agcgcgtgaa agaaacacca aacgcaacct
     2581 ggccggtaca tgctgtaatt accaactcta cctatgatgg tctgctgtac aacaccgact
     2641 ttatcaagaa aacactggat gtgaaatcca tccactttga ctccgcgtgg gtgccttaca
     2701 ccaacttctc accgatttac gaaggtaaat gcggtatgag cggtggccgt gtagaaggga
     2761 aagtgattta cgaaacccag tccactcaca aactgctggc ggcgttctct caggcttcca
     2821 tgatccacgt taaaggtgac gtaaacgaag aaacctttaa cgaagcctac atgatgcaca
     2881 ccaccacttc tccgcactac ggtatcgtgg cgtccactga aaccgcagcg gcgatgatga
     2941 aaggcaatgc tggtaagcgt ctgatcaacg gttccattga acgtgcgatc aaattccgta
     3001 aagagatcaa acgtctgaga acggaatctg atggctggtt ctttgatgta tggcagccgg
     3061 atcatatcga taccactgaa tgctggccgc tgcgttctga cagcacctgg cacggcttca
     3121 aaaacatcga taacgagcac atgtatcttg acccgatcaa agtcaccctg ctgactccgg
     3181 ggatggaaaa agacggcacc atgagcgact ttggtattcc ggccagcatc gtggcgaaat
     3241 acctcgacga acatggcatc gttgttgaga aaaccggtcc gtataacctg ctgttcctgt
     3301 tcagcatcgg tatcgataag accaaagcac tgagcctgct gcgtgctctg actgacttca
     3361 aacgtgcgtt cgacctgaac ctgcgtgtga aaaacatgct gccgtctctg tatcgtgaag
     3421 atcctgaatt ctatgaaaac atgcgtattc aggaactggc tcagaatatc cacaaactga
     3481 ttgttcacca caatctgccg gatctgatgt atcgcgcatt tgaagtgctg ccgacgatgg
     3541 taatgactcc gtatgctgcg ttccagaaag agctgcacgg tatgaccgaa gaagtttacc
     3601 tcgacgaaat ggtaggtcgt attaacgcca atatgatcct tccatatccg ccgggagttc
     3661 ctctggtaat gccgggtgaa atgatcaccg aagaaagccg tccggttctg gagttcctgc
     3721 agatgctgtg tgaaatcggc gctcactatc cgggctttga aaccgatatt cacggtgcat
     3781 accgtcaggc tgatggccgc tataccgtta aggtattgaa agaagaaagc aaaaaataat
     3841 tagctcgtac aagggaagtg gcttgccact tccctttttt gctcataagg agaacacatg
     3901 aaaacaccct cacagccgcg cgcgatatac tatatcgtgg cgatccaaat ctgggagtac
     3961 ttcagttttt acggcatgcg tgccttactc attctctatc tcacccatca gcttggtttt
     4021 gatgataacc atgccatcag cctgttcagc gcatatgctt ctctggttta cgttacccct
     4081 attctcggcg gctggcttgc cgaccgcctg ctcggcaacc gcactgcagt gattgccggc
     4141 gcgctgttaa tgacccttgg ccatgtggtg ctgggcattg atacaaattc aacctttagc
     4201 ctgtatctgg cgctggcaat cattatttgt ggctacggtt tattcaaatc aaacatcagc
     4261 tgtttgcttg gcgagcttta cgacgagaac gatcatcgac gtgatggcgg tttttcgctg
     4321 ctgtatgctg cgggcaatat cggttctatc gccgccccaa tcgcctgcgg cctggctgct
     4381 cagtggtatg gatggcatgt tggctttgcc cttgcgggtg gcggcatgtt tatcggtttg
     4441 ttgattttct taagcgggca tcgtcatttc cagtccacgc gtagtatgga taaaaaagcg
     4501 ctcactagcg tcaaatttgc cttaccggta tggagctggt tagtggtgat gctctgttta
     4561 gccccagtat tttttaccct gctgctggag aacgactggt ccggatattt gctggcgatc
     4621 gtttgcctca ttgccgcaca aatcattgcc cgcatgatgg tcaaattccc cgaacaccgc
     4681 cgtgctcttt ggcaaattgt attgttgatg tttgtcggga cattgttctg ggtactggca
     4741 caacagggcg gcagtaccat cagcttgttt atcgatcgct ttgtgaatcg tcaggcattc
     4801 aatattgaag tacctacagc actattccag tcggtgaatg caattgcggt gatgctcgct
     4861 ggggttgtac tggcctggct ggcgtcgcca gaaagccgcg gcaactcaac attgcgcgtc
     4921 tggctgaagt ttgcctttgg cttactgctg atggcttgtg gctttatgtt gttggcattt
     4981 gatgcccgac atgcagcggc tgacggtcaa gcgtcaatgg gcgtgatgat atccgggttg
     5041 gcgctaatgg gctttgccga actctttatt gacccggtgg cgattgcgca aatcacgcgt
     5101 ctgaaaatgt ctggcgtatt aaccggtatt tatatgctgg caacaggcgc ggtcgccaac
     5161 tggctggcag gcgtcgtggc acagcagacg acagagtcgc aaattagcgg tatggcaatt
     5221 gcagcttacc agcgattctt ttctcagatg ggagagtgga cgttggcttg tgtcgcgatc
     5281 atcgtcgtat tggcctttgc tacccgtttt ctgtttagca cgccgacgaa tatgatacag
     5341 gagagcaacg attaactgtc gtaaaaacaa ttggttatga a
//
LOCUS       DAZFYC010000207         5374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFYC010000207 DAZFYC010000000
VERSION     DAZFYC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.211"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(153..1151)
                     /gene="lpxM"
                     /locus_tag="ACHKW5_004949"
                     /gene_synonym="msbB"
     CDS             complement(153..1151)
                     /gene="lpxM"
                     /locus_tag="ACHKW5_004949"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7514148.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSAHQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            complement(1225..2946)
                     /gene="cptA"
                     /locus_tag="ACHKW5_004950"
     CDS             complement(1225..2946)
                     /gene="cptA"
                     /locus_tag="ACHKW5_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7514149.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETPRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            complement(3036..4142)
                     /locus_tag="ACHKW5_004951"
     CDS             complement(3036..4142)
                     /locus_tag="ACHKW5_004951"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7514150.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     VASAREDIECRFDINRTAQIIVSLASQAKGKCNR"
     gene            complement(4142..4963)
                     /locus_tag="ACHKW5_004952"
     CDS             complement(4142..4963)
                     /locus_tag="ACHKW5_004952"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7514151.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
BASE COUNT         1471 a         1358 c         1179 g         1366 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 ttttgtcata tgagccacct ctgactgaga gtttactcac ttagcgtgtg tccactattg
      121 ctgggtaagc tcattatcct gaaaaacctg cctcagtaag gatcctcttc cccttcttta
      181 cgcgttttca gcaacttgag gatccacgta tactgctcag gatgtggacg aacgaaattt
      241 tcaaccactt cgtttatctg tcgcgcaata tgagcatcac tcttctgtcg tatagcaagt
      301 ggcgggctga catgaatagt cagatgatgg gtctttccgt cataaacagg gaaaagcggt
      361 attatccgtg caccactgat cctgctgagc cgtccgataa ccggtaaagt tgctttatac
      421 gtcgcaaaaa aatctgcaaa ctcactgaat tccgggccat gatcctgatc aggaagataa
      481 tatccccagt atccctgccg gactgagcgg acaaaggagg caataccgtc attacgtgcg
      541 tgcagttttc ctccgaaacg acgacgaaca ctgttccaga cataatccac caccggattt
      601 ctctggttat gaaacatcgc tgccattttt ctgcccgacg cagccatcag cattgcggga
      661 atatccactc cccaggcatg tggcacaaga aaaatgacct tttcattgtt ctgtgccatc
      721 ttctccacaa tctccagacc attccagcgt atacgatgcg aaattttatc aggcccggac
      781 agtgctaatt cagccataag cacaacggcc atggaggctg ttgcaaacat ggcatcaacg
      841 atattctcct tttctttatc actgtactcc ggaaaacaaa gcgataaatt aatcaaggcc
      901 cgttgatgtg cgcttttccc cagacgccca accagcatgc ccaattttcc caacaatgga
      961 tctctcagtg cgggggggac cattgcaaac acacagataa taccgatacc tgtccacgtc
     1021 atccagtaac gggggaagca taatgtaagg ttaaagcggg gaataaactc agttctgata
     1081 tttttcatat ttaccggtat ctgcctgctt atctcattat gataacagag tacagcagaa
     1141 ggtggtacca tccctggagg ccaccggaca gcagaggaaa acacatcctc ctgccttcag
     1201 aacggaaccg aagataagaa aatactatgg ctcaccatac ggaagagtgt cgaaatcagt
     1261 gagcccattc tttttatacg gatttccaat ccagcggggg gtttccctga attgcggatt
     1321 caccagagaa cgggtcggat cgtaaccatc gtatgtcagt cccgccaaat ctgaccaggt
     1381 gtggatcagt tcagccagac tgtatttgcg gtcaacatac tgcgaaaaat ctcgcggatg
     1441 tgcagcatgc cacttttccg aggtccacag caggaacgga acagtgtaca tgggacgtgt
     1501 ggggttatct tcattccgtc cctgcgtttt atatggcgga gtatcatata cttcttcacc
     1561 atgatctgaa aaataaagca gaaagccgtc cggcgcagtt gctctgaagt cctttatcag
     1621 actggccacc acatgatcgt taaacagatt ggcattatcg taatcgttat acacttccag
     1681 ctctttcgca tttaatccag tgggaatatg ccctgtaatg ccatcaaacc gcccctgacc
     1741 ttccggataa cggtatttat acttaatgtg cgtacccagc agatgcacga tgatcagttt
     1801 ctttggtgca gggtcattca gcacttcccg gaacggcttc agcacgttag tgtcatattc
     1861 acgcgcactt tgtgttcgct gctgattcat gtagtactgc ctgtccgtct ggcgcgaaaa
     1921 tacagtgagc atggtgttac gggctgtgat tgtctgctgg ttggtaatcc agaatgtttt
     1981 atagcctgcc tgcttcatca tgttcatcag cgacggctgc gtcagataca gatcaggatt
     2041 cttttcgttg gcgaaagtaa gggcctgttg caatgcttca atggtgtacg gacgcgatgc
     2101 caccacatta ttaaacacag taagacccgg atcggtttta cgcagtgcat ccagctccgg
     2161 cgtcgtttca cgtagatacc cgtataagct catgcgttcg cgctgtgtcg actcaccaat
     2221 caccaggacc agtgtgcgtg gtctctcccc tgattcatcc ctgagattac ccagtggtgg
     2281 cagtgcgcta ttttcattca ggaaggttgt cagtgcattc agttgctgat ggtactgata
     2341 ataactggat acaaactgcc agggggcagc aggctccatt cgggatgcca gtttgcctag
     2401 agtatcattc agcggctcct gtctgattaa cgatttcaga acaaccggat gcagaagcag
     2461 agcataaagc agcaggaaag agacaattct ccgccatggc aaaggaatat atacaggacg
     2521 cagacgtgtc cacagaaaaa cggacaccgc agtatatacc agcgagataa gcaacagttt
     2581 aaggctgaaa tactggctga aatattcacc agcctctctg gcattcgttt caaacataac
     2641 gaaaagaaca ctttgagaga attcatgacc atagagaaaa taataacaaa gtgctgccag
     2701 cgacgtgccc cagagaatga aaccgacaac agctgcaatt atttttatcc gatcaggata
     2761 gagaaacacc gggatcaacc acagacaact gaataacagt gagtcccgta ttccattcgt
     2821 tccgctgtac ccactgctga agatgatgac ctgcaggaga gtggaaaaaa agccaaaata
     2881 aaaaattgcc catcccagcg cactccagct gaaagtaggc ctgttctgtc cggtatttaa
     2941 atgcattgac cgtccccgta tttaaacaat gtgataaatt actccgttac cggaaaaccg
     3001 ctgaacaaaa ttcgggctga aaagaggatc cgccgttatc tgttacattt ccccttagcc
     3061 tgactagcca gagacacaat gatctgtgcc gttctgttaa tatcaaaccg gcactcaata
     3121 tcttctctgg cgctggctac catcatccgg aagcgttccg gtcgggataa aaaatcgcgc
     3181 agtgcgccgg tccatgcaga cacatccccc acgggtaata gcgtccctgt cacattcttc
     3241 tgaatgacat cagggatccc gcccgtctca ctggcgataa cgggcacgcc agagactgac
     3301 gcttcagcca gtaccatacc aaacgcttca ttttccgaag gcatgaccac cacactggca
     3361 atccggtaga ccggtaacgc tgggaaaagg gcacctgcca ttaacacatc tccgctcatt
     3421 cccaggtgtt ctgtctgctg acgcagacgt gcttcgtatt cttcacgccc ggcgcccacc
     3481 acgagccagc gaaatgattt cccttccatc ttcagctgat acaatacacg cagcataaat
     3541 tcatgtcctt tttcgggacg tagcatcccc acctgaacga taagcggaac attgtctgct
     3601 gatgcagccc aggcgtggat atgcaggggt aacggtcgca tggcttcatt atgcaatgcg
     3661 ggccagtcga aacccggtgg aataaccgtt accggtgtcc tgacaccttc cgccatcaga
     3721 tgcgccatca tggctgagct aggcacaaca atgaaatcac acagataatt cagggaaaac
     3781 gttcgggtct tacgggtgat gtaggttttt tgtctgacaa tactgaagcg gtgacagcat
     3841 atcagacggc tcagtcctgc tatattactg tcatggccac tatggcagat gaccagatca
     3901 ggtttaaatt ccccgataat ccgtcgaagt ctgaggatgg aaggaaggtg aaggctgttc
     3961 ctgaaaggga taaaagtgac atcatgccct ctttttctgg cttccggagc aattttactt
     4021 ttttccctgc aggcaagtaa aacggaatgt ccctgctttt gaagagcagt catctgggcc
     4081 agtgcctgca gctcctggcc cccaatatct gacgaagatt cagtgaataa aattttcatc
     4141 attaattatc tggtaatctt ggccccttat gaagagccag tactgaagaa aaaagtggcg
     4201 ttgtataata aaaaaggcgt cgtttcagcc agccggaatg ttcccgctct ttggtgctga
     4261 tacgccctat gcgtaatgaa ccattttcag gacagttcat tcttctttcg gttgtatata
     4321 aataagagaa accaagctta ccggcgagat taatataatc ccgattataa tatccttcag
     4381 gccagcacag atgggaacta cagaacccca gcttttccgt cagacattgt tttcccacca
     4441 gaatatcttc tttcatgagc cggcactgtt cagcacgtga tacagacaac ctgtcccagc
     4501 gtttatgtga atgggtgtgc acatgaaatt caaccagtcc ggaatcacgc atttcccgga
     4561 cttcagacca gcgaagcatg acctcatctg aacggttatc ggcaatcaac cgttcacagt
     4621 cgcggtgaga atattcctgc tcctgcctgc tacgtacgtt tcccttacca atcagccccg
     4681 taatgagaaa aatatgtgca tgaaggttat attccttcag gaccggccat gctctgagcc
     4741 agttatcaag ataaccatca tcgaatgtga gcatgacact ctttcgtgga agcgttcccc
     4801 cctgataaaa atattcaagc tcagcagatg ttaccgtccg ccagttatta tctgcgagcc
     4861 acttcatctg ttcacaaaat gtttcaggtg agagtgtcac aagccccgga caacgactga
     4921 catgatgata catcagtacg ggaagatgcc ttgcattcaa cataaaaaat tatcgtctgt
     4981 taagcattct gaaaatatcc tcgcgtaaac aacagtgagg atccatagat atcacacatg
     5041 gtgatattat tgtgtaatcc cggaatggtc cggagattac cgacaacagg gattttttaa
     5101 tatttttatc agttaatcaa ccagaactta aatttccctt aacgcatatg ctctttttaa
     5161 tcagattttc tgtttttcag aaaaaacaga ataccgcaat ttcagtaaac acagcgagag
     5221 atatccgcct cagtaaaaaa tcagaagatt atcatactga tttattgcat aaaaccagtc
     5281 gagtagcgga attttctgca tccggatacc actccccctg aaaaatactc gcacaatatc
     5341 gcgccgcatg cactgatgga ccgggccatc gtga
//
LOCUS       DAZFYC010000208         5287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFYC010000208 DAZFYC010000000
VERSION     DAZFYC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.213"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..138)
                     /locus_tag="ACHKW5_004953"
     tRNA            complement(52..138)
                     /locus_tag="ACHKW5_004953"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Leu,seq:cag)
     gene            complement(447..1478)
                     /gene="rsmC"
                     /locus_tag="ACHKW5_004954"
     CDS             complement(447..1478)
                     /gene="rsmC"
                     /locus_tag="ACHKW5_004954"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO7514152.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1581..1994
                     /gene="holD"
                     /locus_tag="ACHKW5_004955"
     CDS             1581..1994
                     /gene="holD"
                     /locus_tag="ACHKW5_004955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO7514153.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1963..2409
                     /gene="rimI"
                     /locus_tag="ACHKW5_004956"
     CDS             1963..2409
                     /gene="rimI"
                     /locus_tag="ACHKW5_004956"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7514154.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2424..3101
                     /gene="yjjG"
                     /locus_tag="ACHKW5_004957"
     CDS             2424..3101
                     /gene="yjjG"
                     /locus_tag="ACHKW5_004957"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO7514155.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3486..4709
                     /locus_tag="ACHKW5_004958"
     CDS             3486..4709
                     /locus_tag="ACHKW5_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514156.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKAPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGEITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQVWW
                     ERVESLVTCPSSDSS"
     gene            complement(5081..>5287)
                     /locus_tag="ACHKW5_004959"
     CDS             complement(5081..>5287)
                     /locus_tag="ACHKW5_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HFO7514157.1"
                     /translation="KGNIIIEDLFNFYNYRDNIEFISPQLYRTLPNKINPIYQELKKR
                     AIVDYISGMMDTFAIREWETHHKK"
BASE COUNT         1354 a         1254 c         1335 g         1344 t
ORIGIN      
        1 tctaccaatt ccgccacctt cgcccagtgc gagcaatatc aacgtggttt ttggtgcgag
       61 gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac
      121 caattccgcc accttcgcat accatcaatt cttaaaaaga attgctacca cggaggcgca
      181 ttctagtggt tttcagcttt tcgtcaatag ttaattatcg ccggaggtgt aattgctgga
      241 aaaatgtcca tcaggaaact aacgtgcagg tttggtagtt ttgtaggtcg gataaggcgt
      301 tcacgccgca tccgacacgg tatttgggtg cagatgccag atgcgacgcg ggcgcgtctt
      361 atctggccta cgaagggcta acgtgcaggt tttgtaggtc ggataaggcg ttcacgccgc
      421 atccgacacg gtattcggcg agataattaa cctttcttcg cctggcgggt cataatggcg
      481 cgatacacct tgaaacgccc ggtttgcgca atcacttcat ggaagccaaa tgtctcatcc
      541 agcacgtccg ggtaaggcag gaaggcgttc gctacaattc gcagctcgcc gccgctatta
      601 agatgacgca ccgcgccgcg aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca
      661 tcgtggaacg gcgggttgga gatgatcata tcaaaacgac ctttcacctc ggaaaagacg
      721 ttgctggcaa agacttcacc ttcaacaccg ttggccgcaa gtgttgcgcg gctggcttct
      781 accgctggcg cagagacatc gcacaaggtg agacgaattt tcggcgaatg gcgcgcaaag
      841 gcaactgaaa gcacacccgc gccacagccg acatccagca ctttaccttt cgtgtgcggg
      901 gttaacgtcg agagcagcaa ctggctaccg acatccagac cgtcgcggct aaacacgcca
      961 ggcagcgttt tgaccgtcag gccatcgacg ctgtattcgc cccagaattt atcggcatca
     1021 aataccggct gtttttccag acgaccaaaa tagaggccac agcgacgagc gctgtcgact
     1081 ttattcaacg gcgcataatc tgccagcatc tgctcggcgc tgcgcacgcc gctgcggttc
     1141 tcgccaacga caaaaatatc tgtccccact ggcagcagag aaagtaaatt catcaactgg
     1201 aactgggctt ccggtttgtt cttcggccag tagtaaatca gcgtatcgca atctgcgacg
     1261 tcatccaccg tggcgaccag actgaaacgg gcgttatccc ccatctggcg gcttaatacc
     1321 tgccagtggt ggaattgctg ggtatgagca cggctggccg cggtatctaa acgcgcgggc
     1381 aggtcatcct gtaagtctcc ggcaaacaga atacggcttt gttcgaaatc atcactgtga
     1441 cgcagcaaga cttcacttgc cggggtaaat gcagacatgg aatgctcctc aattgatact
     1501 ggcggcgatt atagccatat gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct
     1561 gacaacagga gcgattcgct atgacatccc gacgagactg gcagttacag caactgggca
     1621 ttacccagtg gtcgctgcgt cgccctggcg cgttgcaggg ggagattgcc attgcgatcc
     1681 cggcacacgt ccgtctggtg atggtggcaa acgatcttcc cgccctgacc gatcctttag
     1741 tgagcgatgt tctgcgcgca ttaaccgtca gccccgatca agtgctgcaa ctgacgccag
     1801 aaaaaatcgc gatgctgccg caaggcagtc gctgcaacag ctggcggttg ggtactgacg
     1861 aaccgctatc actggaaggc gctcaggtgg catcaccggc gctcaccgaa ttacgggcaa
     1921 acccaacggc acgcgccgcg ttatggcaac aaatttgcac atatgaacac gatttcttcc
     1981 ctcgaaacga ctgatttacc ggcggcttac cacattgaac aacgcgccca cgcctttccg
     2041 tggagtgaaa aaacctttgc cagcaaccag ggcgagcgtt atctcaactt tcagttaacg
     2101 caaaacggca aaatggcggc gtttgcgatt acgcaagtgg tgctggatga agctacattg
     2161 ttcaatattg cggtcgatcc tgactatcag cgtcagggat tgggaagggt gctgctggaa
     2221 catctgatcg acgaactgga aaaacgcggc gtggcgacac tatggctgga agtccgtgcc
     2281 tcaaatgctg ccgccattgc cctgtacgaa agtttaggct ttaacgaggc gacgattcgc
     2341 cgcaattact accccaccac ggacggtcgc gaagacgcca tcatcatggc gttgccaatc
     2401 agtatgtaag acaaggtgga ataatgaagt gggactggat tttctttgat gccgatgaaa
     2461 cgctgtttac ctttgactca ttcaccggcc tgcagcggat gtttcttgat tacagcgtca
     2521 cctttaccgc tgaagatttt caggactatc aggccgttaa caagccactg tgggtggatt
     2581 atcaaaacgg cgcgatcact tcattacagc ttcagcacgg gcgtttcgag agctgggccg
     2641 aacggctgaa cgtcgagccg ggtaaactca acgaagcctt tattaatgcg atggcggaaa
     2701 tctgcacgcc gctgccgggc gcggtttctc tgcttaacgc cattcgtggc aacgccaaaa
     2761 tcggcatcat caccaacggc tttagtgcct tgcaacaggt gcgtctggaa cgcacgggcc
     2821 tgcgtgatta cttcgatttg ctggtgattt ccgaagaagt tggcgttgcc aaaccgaata
     2881 agaaaatttt cgattatgcg ctggaacagg cgggcaatcc tgaccgttca cgcgtgctga
     2941 tggttggcga cactgccgag tccgatattc tcggtggcat caacgccggg cttgcgacct
     3001 gctggctgaa tgcgcacaat cgcgagcaac cagaaggcat cgcgcccacc tggaccgttt
     3061 cttcgttgca cgaactggag cagctcctgt gtaaacactg attgcctccc ccccgttgat
     3121 gggtaaaata gccgcaattt ttcgttttca acaagcgcgg cgcgatgccg cttactcaag
     3181 aagaaagaat tatgacgttg tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg
     3241 ccattatttc tcaccccaat aattaagccc aaattaaagc tcttttactc tttcaaaatc
     3301 ctttcagtta attgagtttt ggtcatataa atcatttaaa tacatatatt tgtgtgactt
     3361 tttaattgaa gattctttca tgtaacttta aatcgatcag gttgctttca tcaaaaatct
     3421 gtacatatgc ttgtacataa tgtacaaagc agcagaggtg ttttgcgatt tgtacaaggt
     3481 gagtaatggc gctgtctgat gcgtggttgc gttcagtcgt tggaaaggaa cgtgataagg
     3541 ttttggttaa atccgatcgt gatggtctgt ctgtcagagt atcaccgaaa ggtcgcgtag
     3601 tattccaata tcgttatcaa tgggcaggga aaggtgagcg tcttgatatc ggaacttacc
     3661 cggcaactgg attaaaagag gccagagaag aagttatccg tctccgtggt gaactcgagt
     3721 caaaccgtaa tccacgattg gtcaagcagg ctgaaaaacg aaaagctact gaagccatga
     3781 cggtagagtc tgtgatccgt gcctggtatg aagcatattg tgtaaaaaat aaaaagggtt
     3841 ctgaacagat actccgctcg tttgagttgc acctgttctc taaaatcggg aatatccctc
     3901 acgatgcagc tacattgcat gattggttag aagtcctgga gcctcttagc actaaggctc
     3961 cagcaatagc agaccgatta ctaattaacg caaagcaggc ccatgtctgg gcgtataaga
     4021 gaaagctcat tgaaactcgc ccgctgtcgg atatcacggg taaagatatg gatatccgta
     4081 aaggtcagaa gaaacggttt ctgacacatg atgaaattaa aatcctttat gctgcgatcg
     4141 atggttctcg aatggttcct aaataccggg ccttcattaa actattgctg cattttggct
     4201 gccgtagttc agagctaatt accgccaggg tggacgattt tgatttcatt aataaggtat
     4261 ggactgtacc gccagaacga cataagacag gggagataac aggcgaacca ctaaagcggc
     4321 ccattattga accggttgaa gagcttataa aatacgttat ctctatgaac aacggttccg
     4381 atatgctttt tactaaggaa ggaagcaggg agccagttgg tcggacatca ttgcagtcgc
     4441 tgccttacaa tttaatgcag ttcgcatggc gacgtttggg gtatcaattc cctcattggt
     4501 ctcttcatga tttgaggcga acagcacgaa caaacttttc tgatcttact gcgcctcata
     4561 ttgcagaaat tatgcttggt cataagctgc caggggtatg gcaggtttat gacaagagcg
     4621 attatctaga agaacagcgt aaagcctacc aggtatggtg ggagagggtt gaatcgctcg
     4681 ttacttgtcc tagttcagat tccagttgac aatttgcgca gccagaacgc aataaaatat
     4741 gactattggc gttgagcagt gagcttaacg gagagataca acttacagcg caaagtgtat
     4801 atttaatgtt tagaaaacgt acggtttaag ttacgaaatg taaagtaaag atgattggtt
     4861 acgatttaac acactccgtt acttgatatt atttttgcat atgtcctttc tttttggtta
     4921 aaaataacga tgtgcacgga agcgtaatca atgtggaaaa tgctagctca gaactactag
     4981 atgtggctta tctgtataat cgggtgataa tggacagaat cactgttctg tcccatggga
     5041 agagtggcac tgaatctgtt gttgatgtgc gccagtttgg ttattttttg tgatgcgttt
     5101 cccattcgcg gatggcaaat gtatccatca ttccagagat gtagtcgaca attgcacgtt
     5161 ttttaagttc ttgataaata ggatttattt tatttgggag cgttctatac agttgagggg
     5221 atatgaattc tatgttatct ctataattat aaaaattaaa taaatcttcg atgataatgt
     5281 ttccttt
//
LOCUS       DAZFYC010000209         5243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFYC010000209 DAZFYC010000000
VERSION     DAZFYC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.214"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..252
                     /locus_tag="ACHKW5_004960"
     CDS             <1..252
                     /locus_tag="ACHKW5_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514158.1"
                     /translation="LKIQNKFYVKIKYKEDIFFLRKNIDLLQELSGTIDVILGSRFIK
                     ELKESRSFSRVEISESELWGISNNPVNHAHQRNPNKKWS"
     gene            441..809
                     /locus_tag="ACHKW5_004961"
     CDS             441..809
                     /locus_tag="ACHKW5_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO7514159.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
     gene            882..1046
                     /locus_tag="ACHKW5_004962"
     CDS             882..1046
                     /locus_tag="ACHKW5_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO7514160.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            1015..1158
                     /gene="kil"
                     /locus_tag="ACHKW5_004963"
     CDS             1015..1158
                     /gene="kil"
                     /locus_tag="ACHKW5_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO7514161.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            1233..1529
                     /locus_tag="ACHKW5_004964"
     CDS             1233..1529
                     /locus_tag="ACHKW5_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7514162.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIGHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1535..2320
                     /gene="bet"
                     /locus_tag="ACHKW5_004965"
     CDS             1535..2320
                     /gene="bet"
                     /locus_tag="ACHKW5_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7514163.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2317..2997
                     /locus_tag="ACHKW5_004966"
     CDS             2317..2997
                     /locus_tag="ACHKW5_004966"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7514164.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2994..3176
                     /locus_tag="ACHKW5_004967"
     CDS             2994..3176
                     /locus_tag="ACHKW5_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO7514165.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPDLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            3149..3340
                     /locus_tag="ACHKW5_004968"
     CDS             3149..3340
                     /locus_tag="ACHKW5_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7514166.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            3417..3632
                     /locus_tag="ACHKW5_004969"
     CDS             3417..3632
                     /locus_tag="ACHKW5_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7514167.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            3731..3952
                     /locus_tag="ACHKW5_004970"
     CDS             3731..3952
                     /locus_tag="ACHKW5_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7514168.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(4163..4765)
                     /locus_tag="ACHKW5_004971"
     CDS             complement(4163..4765)
                     /locus_tag="ACHKW5_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514169.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWTLNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            5008..5175
                     /locus_tag="ACHKW5_004972"
     CDS             5008..5175
                     /locus_tag="ACHKW5_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514170.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
BASE COUNT         1600 a         1161 c         1209 g         1273 t
ORIGIN      
        1 ttaaaaatac agaataaatt ctatgtaaaa attaaatata aagaagatat tttcttcctc
       61 agaaaaaaca tagatctttt acaagaactt tctggtacaa tagatgtcat cttaggttct
      121 aggtttataa aagaactgaa agaaagcagg tctttctctc gtgtagagat aagcgagtca
      181 gagttatggg gtatatcaaa taatcctgta aaccacgctc accaacgaaa cccaaacaaa
      241 aagtggtcat aacccgctca ggcggatttc attttcacgc aaacaacaga ataaacacag
      301 cgctgtgtat tcattccaac gagtgaatac acggagcaat gtcgctcgta actaaacagg
      361 agccgacttg ttctgattat tggaaatctt ctttgccctc caagatgagg gcgatttttt
      421 atctgtgagg atatgaacag atgtcaaaca tcaaaaaata catcattgat tacgactgga
      481 aagcatcaat agaaattgaa atcgaccatg acgtaatgac agaggaaaaa cttcaccaga
      541 ttaataattt ctggtcagac tctgaatacc gactcaataa acacggctct gtattaaatg
      601 ctgtattaat catgctggcg caacatgctc tgcttatagc aatttcaagc gacttaaatg
      661 catatggtgt tgtgtgtgag ttcgactgga atgatggaaa tggtcaggaa ggatggcctc
      721 caatggatgg tagtgaagga ataagaatta ccgatatcga tacatcagga atatttgatt
      781 cagatgatat gactatcaag gccgcctgag cgcggcgtta ccgcatacca attacgcttc
      841 actcgaggcg tttttcgtta tgtataaata aggagcacac catgcaatat gccattgcag
      901 ggtggcctgt tgctggctgc ccttccgaat ctttacttga acgaatcacc cgtaaattac
      961 gtgacggatg gaaacgcctt atcgacatac ttaatcagcc aggagtccca aaaaatggat
     1021 caaacaatta tggctatcca gactaaattc actatcgcca cttttattgg cgatgaaaag
     1081 atgtttcgtg aggccgtcga cgcttataaa aaatggatat taatactgaa actgagatca
     1141 agcaaaagca ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc
     1201 gatattttca cagctatttc aggagttcgg ccatgaacgc ttattacatt caggatcgtc
     1261 ttgaggctca gagctgggcg cgtcactacc agcagatagc ccgtgaagag aaagaggcag
     1321 aactggcaga cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg
     1381 gtcatttgca acgccacggg gccagcaaaa aagccattac ccgtgcgttt gatgacgatg
     1441 ttgagtttca ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc
     1501 atcaggttga tattgattca gaggtataaa acggatgagt acagcactcg caacgctggc
     1561 agggaagctg gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac
     1621 tcttcgccag acggcattta aaggcgatgc cagcgatgcg cagttcatcg cattgttgat
     1681 cgtcgccaac cagtacggcc ttaatccgtg gacgaaagaa atttacgcct tccctgataa
     1741 gcagaacggc atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa
     1801 ccagcagttt gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta
     1861 ccgcaaggac cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga
     1921 accattcaaa acccgcgaag gcagagaaat cacggggccg tggcagtcgc atcctaaacg
     1981 gatgttacgg cataaagcta tgattcagtg tgcccgtctg gccttcggat ttgctggtat
     2041 ctatgacaag gatgaagccg agcgcattgt cgaaaatact gcatacactg cagaacgcca
     2101 gccggaacgc gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct
     2161 aatcgccctg gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg
     2221 ccgcgacatt cgcgcatcgt cagaactgac acaggccgaa gcagtgaaag ctcttggatt
     2281 cctgaaacag aaagccactg agcagaaggt ggcagcatga caccggacat tatcctgcag
     2341 cgtaccggga tcgacgtgag agctgtcgaa cagggggatg atgcgtggca caaattacgg
     2401 ctcggcgtca tcacagcttc agaagttcat aacgtaatag caaaaccccg ctccggaaaa
     2461 aagtggcctg acatgaaaat gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt
     2521 gtggctccgg aagttaacgc taaagcgctg gcctggggaa aacagtacga gaacgacgcc
     2581 agagccctgt ttgagttcac ttccggcgtg aatgttactg aatcaccgat catctatcgc
     2641 gacgaaagta tgcgtaccgc ctgctctcca gatggtttat gcagtgacgg caacggcctt
     2701 gaactgaaat gcccgtttac ctcccgggat ttcatgaagt tccggctcgg tggtttcgag
     2761 gccataaagt cagcttacat ggcccaggtg cagtacagca tgtgggtgac gcgaaaagat
     2821 gcctggtact ttgccaacta tgacccgcgt atgaagcgtg aaggactgca ttatgtcgtg
     2881 gttgagcggg atgaaaagta catggcgggt tttgacgaga tggtgccgga gttcatcgaa
     2941 aaaatggacg aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgatgaagc
     3001 atcctcacga taatatccgg gtaggtgcga tcactttcgt ctactccgtt acaaagcgag
     3061 gctgggtatt tcccgacctt tctgttatcc aaaatccact gaaagcccag cggctggctg
     3121 aggagataaa taataaacga ggggctgtat gcacaaagca tctcctgttg agttaagaac
     3181 gagcattgag atggcacata gccttgctca aattggagtc aggtttgtgc caataccagt
     3241 agaaacagac gaagaatttc atacgttagc cacatccctt tcacaaaagc tggaaatgat
     3301 ggtggcgaaa gcagaagcag atgagagaga ccaggtatga caaccactga atgcattttt
     3361 ctggcagcgg gcttcatatt ctgtgtgctt atgcttgccg acatggggct tgttcagtga
     3421 cacctcagca agaaaacgcc cttcgcagca ttgcccgtca ggctaattct gaaatcaaaa
     3481 aagccagaca gcagtttccg gataaaaacg tcgatgacat ttgccgtagc gtactgaaga
     3541 agcaccgcga aacggtaacg ctgatgggat tcacaccgac tcatttaagc ctggcgatcg
     3601 gcatgttaaa cggcgtcttt aaggaacggt gagcatgaaa agcaaaatca tcagggagct
     3661 acaggctcct tttttattgt tcgcattcac cctcaagcgt attaaccaac aattcaggga
     3721 ttaatggaag atggcagaca tcattgattc agcatcagaa attgaagaat tacagcgcaa
     3781 cacagcaata aaaatgcgcc gcctgaacca ccaagctata tctgccactc attgttgtga
     3841 gtgtggcgat cccatagatg agcgaagacg cctggccgtt cagggttgtc ggacttgtgc
     3901 aagttgccag gaggatctgg aacttatcag taaacagaga ggttcgaagt gagcgaaatt
     3961 aactagaagc caaagataaa atcatcgctg agcaggagaa aatcgctaac ggagaaaaga
     4021 cagtaagtca gtatatgaaa accgcatgat atcatcagat aaaaatcgat cgtaaagcga
     4081 aatattaata ccagaataaa cgagtcgagg taaattatat tacctcgata aattaactaa
     4141 aacttgcccg ctatatacta tctcattcag tatcatcacg cgcggtctgt gcatatgtta
     4201 ctaccgcacc taatgtatta attttctttt caacatagat aatattatcg tactcataat
     4261 tgccatacgg atagcaaatg cgaatattct catgtagatc ggggtcatcc acctcagctc
     4321 cagaacaact ttttgaacta ccggaagtat accgatacgg tgcaacataa gacgatgtct
     4381 ctccaggcaa aaaataagtt agtgttgtaa ggggtataat cagaaaaaat ccagcaaata
     4441 tgcacatccc tgcataaacc ttaaggtatg ctgacagact cttccagccg ctttgtttta
     4501 ctatcccctt cttaacccaa aacagagata acagaaaagc tattcccatg ctaaacagaa
     4561 tgtaatagtg ggatatactc tgattaagaa acgtgaccct gtaaatatct gcccgccacc
     4621 agaagaaaag gaaaataaag atcagacctg aaactgtcat gcaaatcaaa taaggatacg
     4681 aatctttttt catgtttagc gtccataaaa tttttcctgc cccggacaaa tttaccatcc
     4741 attttttgcg cagaaaatag ctcattactt actgcacaat aatacacaaa attgcgtaaa
     4801 ttttttgcat ggattttagc tctttcagcc gacgtttaag gggtaaatag catttcctaa
     4861 aagcaactgc accaacccaa cagaatgagc taccgcttac gttgagagca aaaaagtgta
     4921 tagcagcaat gaacagcatc ctcgcactga cgaggatttc ttttatctga actcgctacg
     4981 gcgggttttg ttttatggag atgataaatg cacttccgag tcacaggtga atggaatgga
     5041 gaaccattca acagagttat cgaagcagag aacatcaatg actgctatga ccactggatg
     5101 atatgggcgc agatagcaca tgcagacata accaatattc gaattgaaga actgaaagaa
     5161 caccaagccg cctgatggcg gctttttctt gcgtgtaatt gcggagactt tgcgatgtac
     5221 ttgacacttc aggagtggaa cgc
//
LOCUS       DAZFYC010000210         5218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFYC010000210 DAZFYC010000000
VERSION     DAZFYC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.215"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..604)
                     /locus_tag="ACHKW5_004973"
     CDS             complement(<1..604)
                     /locus_tag="ACHKW5_004973"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7514171.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRVADLMEVINGRAYKKHEMLQTGTPLLRVGNLFTSNEWYYSDLQLDENKYIN
                     NGDLIYAWSASFGPFIWTGEKVIYHYHIWKLNLFAEEYSNK"
     gene            complement(604..2073)
                     /locus_tag="ACHKW5_004974"
     CDS             complement(604..2073)
                     /locus_tag="ACHKW5_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO7514172.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            complement(2140..4572)
                     /gene="hsdR"
                     /locus_tag="ACHKW5_004975"
     CDS             complement(2140..4572)
                     /gene="hsdR"
                     /locus_tag="ACHKW5_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO7514173.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDEEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            4850..>5218
                     /locus_tag="ACHKW5_004976"
     CDS             4850..>5218
                     /locus_tag="ACHKW5_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO7514174.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENEYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKIIPIQGIKDDLQKR
                     VLPIDVYSDETDEPRLIVRKK"
BASE COUNT         1228 a         1216 c         1247 g         1527 t
ORIGIN      
        1 atttgtttga gtactcttct gcaaataaat ttaatttcca tatatggtag tgataaatta
       61 ctttttctcc tgtccaaatg aatggcccaa aagatgcgga ccaagcgtat attaaatcac
      121 cattatttat atatttgttt tcgtctaatt ggaggtcaga gtaataccac tcatttgatg
      181 tgaatagatt accaacacgc agcaaaggag tgcctgtttg aagcatttca tgttttttat
      241 atgctcgacc gttaataacc tccatgagat ctgcaacccg cacccactcc catccctccg
      301 gcaattcaaa cggcttctct tcctcgctaa tttccggcag tggtttttgc tttttaattt
      361 taccttgctt caccagctct gctttttccg ccgcaatacg cttcagcagc tccgacgccg
      421 gttcatcgtt cgggtcttgc ggcaccagtt taccgcgcac tgccagttcc agaatcagct
      481 cacgtaattt cttaatgcca tacaaatcaa ttttaccgga actgccgcgc ccggcggtgg
      541 aacgggtttg caacgccgag gtccaggttt ccatatgatc aacgatcagc ttttccacac
      601 tcatcagtta acctccttgc cagacagcgc agcgccgaga atatcgcgca gttgattacg
      661 cagcgtctgg atctccgcct gttgctttgc atactgcgct aacagttcgt ccggatcatg
      721 gctgacggtt tccgcctgat gtgggttttt aatatccaga ttgaagttac gcgcaatcac
      781 gtcatcaatg ctgactttcc acgcttgctc attctcgacg cggctggcaa aaccatcggc
      841 ctcgttaccc caccagtcga tctccgcctg aaactcttca aacttcatcg gcttggtttt
      901 gctgtagttt ttcacgcccg ccggatacgg atgctcatag aaccaaatct ctttggttgg
      961 ctgacctttg gtaaagaaca acaggttggt tttaatgccg gtataggggt taaacacccc
     1021 attcggtaaa cgcacgatgg tatgcaggtt gcactcttcg gtaagtagct ttttgatttt
     1081 ggttttaacg ccttcgccaa atagtgtgcc atccggcaat accaccgccg cacgaccgtt
     1141 tttcgccagt acttccacaa tcagttgcag gaacagatcc gccgtttcgc gggtttgcat
     1201 ctctgccgga aagttcttct caataccgtc ttcttccgtg ccaccaaacg gcgggttggt
     1261 aacaatgaca tccagttgtt catcccagga ggaaagcggt ttgttcaggg tgttgtcgtg
     1321 acgaatttgt accggtactt caatgccgtg cagcagcata ttggtggtcg ccagcaggtg
     1381 cggaagctgt tttttctcaa caccgtggat ctgttgttgc agcgtctgat gatcggcgac
     1441 gctcttcacg tatttgttct ttacatgatc aaatgcgcag gcaagaaaac cgcccgtacc
     1501 gcaggccggg tccataatgg attcgccgag tttcggatca acgcgatcca ccataaagcg
     1561 agtgacggcg cgtggagtat agaattcgcc agcattgccc gcagattgca gatctttaag
     1621 gatctgttcg taaatatcac caaacagatg gcgttcgctg gcgctggtaa agtcaatttc
     1681 gttcagcttg ttgatcacct gacgcagtag cgtaccgttt ttcatatagt tataggcatc
     1741 gctgaacgcc tgcttcacca cgtagccgcg tgggttttta tcgataggtg cagtgaggtt
     1801 tttcaacgcc gggaacagat catcattaac gaattccagc agggaatcac cggtaatgcc
     1861 ctgagcgttt gcagcccaac tgcgccacaa ataacgctgt gggatcggat attgatagtt
     1921 atcctgctcc agttccagcg cctcttcctg ggcatcgaag attttcaaaa acagcagcca
     1981 ggagagctga ccgagacgct gcgcatcgcc gtccacaccg gcatctttgc gcataatatc
     2041 ttgtaatgat ttgattactg aactgataga catgttgtat tccatgaaaa acaaggcctt
     2101 ctgcaacatg atggttgcag aaggcgcaaa agagagttgt taagcagagc ggggcggtaa
     2161 ttggtagatt tcgttttcca gctcgctgag cgcctgatta tacccgttac ggtcgccaaa
     2221 tccggtttta ataatctccg gtaaggtgcc catgctgtcg aatggtttaa gtttcagcac
     2281 ctgaatactt tcgatctcct gtacgcccgc atcggcgtat ttatccagca gattatcgag
     2341 cacggcttgc gctgcttcag agtattttgt gaagtagttc cgcttgcgca cattctcggc
     2401 gcgctctttg cgggttaacg gcggctgacc atacactacg tggcaaagca tgtcgaatgg
     2461 gtcgagatct ttaccgactt cttctgccag tacttcccag atgatcccct gttgctccag
     2521 ctcgtgaatg atcgcttctt tgcgatcggc gtcctgccac ttgcgggtaa agtcatccag
     2581 cgaggcatat tctttgagca gtgttttgcg ggtgtaatct ttaaaggatt cggtaaccag
     2641 tttaccgtcg gcgtcgtaat actgaacacg ctgtgcaata acgcccaccg ctacaccgtt
     2701 gacgtgaaac ttgcggattt tcttctcgtc ttcttccgga agtgggccga catcatcggt
     2761 atcggtaacc tggtatggtg gcgcaggcgg ttcatcaacg cctgttacct gttcttcgtc
     2821 atgttcgctg atttcttcga gtttctcttc aaaatcagat tctggatcgg cgatatcctc
     2881 tggtgtggta tccatgactt tttcgggaat gccatcgaaa cgctcatcgg caaacagttc
     2941 ggtggctttt ttaaagtcga ggatggtaaa ccagagtttg ccgtaacgtt cgtcgatgcg
     3001 tgtaccacga ccgataattt gcttgaattt ggtcatcgac tggatgttct ggtccagtac
     3061 taccagtttg caggttttag catccacacc ggtggtcatc agctctgaag tggtcgcgat
     3121 aaccgggtac ggttttttcg ggttgatgaa gttatccaac tgagctttgc caatttcatc
     3181 atcgccggtg attttcatga cgtatttgtc gttctttttc acctgctccg ggttgagatt
     3241 aaccagggcg cggcgcatac gttctgcatg atcgatgtcg ttacagaaga cgatggtttt
     3301 atccatcggg ttggtgcgct tgaggtagtc ggtaatggtt ctggcaacca gttctgtgcg
     3361 ttcgtcgatt accatcgtgc gatcgaaatc tttctggtta tagatacgat cgtcgatcac
     3421 ctcgccgttt aagtcagttt gccctttggt tgggcgccag ccttgcagat caacatcaat
     3481 atcaacacgg acaactttat aaggggcgag gaagccgtct tcgatccctt cttttagcga
     3541 gtagacgtaa accggatcgc cgaagtaatc cgtgctggag acttcatgcg tctcttttgg
     3601 cgtggcggtt aagccaattt gggtagctga actgaaataa tcaaggatct ctcgccaggc
     3661 gctgtcttcg gatgcgctgc cgcgatggca ttcgtcgatc acgatcagat cgaagaaatc
     3721 tggtgcgact tgtttaaacg ctttttggtc ttcctccggg ccagttatag cctgatagag
     3781 cgcgaggtga atttcataag cgggatcaat ggtgcgtccg ctgactttgg tcattgccgt
     3841 accaaatggc tggaaatcat tatttttggt ttggtcgacc agaatgttgc gatcggcaag
     3901 gaacaaaatg cgttttttat tttttgattt ccacaggcgc cagatgatct ggaatgcggt
     3961 ataggttttc cctgttccgg tcgccatgac cagcagaacg cggttttgcc cgttggagac
     4021 ggcttcaatg gttttgttga ttgcctgaag ctggtaataa cgtggcgatt taccgctgcc
     4081 atcgtcgtaa tagtcctgag taatcaccgg aagctgagct tgtgtgtatc ctttccagag
     4141 gcagaatttt tgccagagtt cagcggggga ggggaagtca tccagcgtta tttgcttttc
     4201 aaggcattca ccttcggctg cggtggcatc gcggaagata aagccatccc cgttggtggc
     4261 aaaaacaaaa ggaacgtcca gcaggcgcgc atattcaatg ccctgttgca tccctttgcc
     4321 aatttcatgt ttgttggctt ttgcttcaat cacggctaag ggaatgcctg gtttgtgata
     4381 cagcacgatg tcagcagatt ttaccgttct gcgtgccgca actttaccac gtacgatgac
     4441 tttaccgtcc cggagtttga cctcctgtct gatttgcgtt gtgttgtccc agcctgcatt
     4501 gagaatggct ggcataacgc atttggtaat gatgtctgct tccgtcaggt tacttaggtt
     4561 cagttccgcc atggggtgtt ctcgctttta tcaggacgat tctgattgta cataaaatca
     4621 atagcctggc tatgatttag ggcggtgttt caacgtaaag tacgtagcag agatcacatt
     4681 gatatctctg ttatatcttg cttgaattaa tctgggcata tcagagacag ataaacagaa
     4741 gttacaggaa ccatatcaac tctaacaaaa tatataaaaa tcttatagtt gcgtttcttt
     4801 cgagcaagaa agataatgtt attgttgatt gtttgattgg gtatatgaaa tggaagctaa
     4861 agaatgtaag gtgcaggata tccttactga aaacaaaaag tttattattc cttcttatca
     4921 gcgtccttac agttggacgg tagataatgc agagcaactc attgatgaca tttataaaag
     4981 ctctcaatca gaagagaatg aatattttat tgggagtatg atttgtatca ataaagggca
     5041 aaatcagtat gaagttgtag atggacaaca acgtcttaca acgttaagca ttattgtctc
     5101 cgagctaaaa aaaatcatcc cgattcaggg gataaaagat gatttgcaaa aaagagttct
     5161 gcctattgat gtttattctg atgaaacaga tgaaccaaga ctaattgtta gaaaaaaa
//
LOCUS       DAZFYC010000211         5014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFYC010000211 DAZFYC010000000
VERSION     DAZFYC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.216"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..500
                     /gene="cesT"
                     /locus_tag="ACHKW5_004977"
     CDS             30..500
                     /gene="cesT"
                     /locus_tag="ACHKW5_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO7514175.1"
                     /translation="MSSRSELLLDRFAEKIGIGSISFNENRLCSFAIDEIYYISLSDA
                     NDEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLD
                     DATPEKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            561..3407
                     /gene="eae"
                     /locus_tag="ACHKW5_004978"
     CDS             561..3407
                     /gene="eae"
                     /locus_tag="ACHKW5_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type epsilon"
                     /protein_id="HFO7514176.1"
                     /translation="MITHGFYTRTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADNVDTITYTATVKKNGVAQANAPVTFSIVSGTATLGANSAKTDGNGKATVTL
                     KSGTPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKANGSDAITYIV
                     KVMKNNQPEANHSVTFSTNFGNLGGNSNTQIVKTDKDGRATVKLTSGVAGNAVVSAKV
                     SEVNTEVKAPEVKFFSVLSIDSNVSIIGTSANGALPNIWLRYGQFKLTAKGGDGKYQW
                     RSQDPSVASVDALTGRVTLLKKGTTTIEVVSGDNQTAMYTINTPTKFISVETQNKVVY
                     SDAEATCRMNNARLPSSTSELKDVYNKWGAANSYEGYKGKKTITAWTQQNEDDKQKGW
                     TSTFDIVTKNEIPSNGSNSKVHVNKANAFAVCVR"
     gene            complement(3681..4901)
                     /gene="escD"
                     /locus_tag="ACHKW5_004979"
     CDS             complement(3681..4901)
                     /gene="escD"
                     /locus_tag="ACHKW5_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO7514177.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT         1523 a          921 c         1080 g         1490 t
ORIGIN      
        1 aattataaat tcaattaaga gaaattataa tgtcatcaag atctgagctt ttattagata
       61 gatttgcaga aaaaattggt attggatcta tttcatttaa tgaaaataga ttgtgttctt
      121 ttgctattga tgagatttat tatatttcgt tatctgatgc taatgacgaa tatatgatga
      181 tctatggcgt ctgtgggaaa ttcccgacag ataatcctaa cttcgctctt gagattttga
      241 atgcaaactt atggtttgca gagaatggtg gaccatacct gtgttatgaa tcaggagcac
      301 aatcgctgtt gttagcgtta cgttttccgc tcgatgatgc tacacctgaa aaacttgaga
      361 atgaaataga agtcgtcgtt aagtcaatgg aaaacctgta tttggtatta cataatcagg
      421 gaataacatt agaaaacgaa catatgaaaa tagaggaaat tagttcaagc gataataaac
      481 attattacgc cggaagataa aagattattt attaatataa tttactattc attctaactc
      541 attgtggtgg agcccataac atgattactc atggttttta tacccggacc cggcacaagc
      601 ataagctaaa aaaaacattt attatgctta gtgctggttt aggattgttt ttttatgtta
      661 accagaattc atttgcaaat ggtgaaaatt attttaaatt gagttcagat tcaaaactgt
      721 taactcaaaa tgccgctcag gatcgccttt tttatacgtt aaaaacaggt gaaactgttg
      781 ccaatatttc taaatcacag gggatcagtt tatcggtaat ttggtcactg aataaacatt
      841 tatacagctc cgaaagcgaa atgatgaagg ctgggcctgg tcagcagatc attttgccac
      901 tcaaaaaact gtctgttgaa tatagtgcct tacctgtctt aggttcggca cctgttgttg
      961 ctgcaggtgg tgtcgctggt catacgaata aaatgactaa aatgtccccg gacgcgacta
     1021 aaagcaacac gaccgatgac aaggctctaa attatgcggc acaacaggcc gcgagccttg
     1081 gtagccagct ccagtcgcgc tcactgaacg gcgattacgc gaaagatacc gctcttggta
     1141 tggccagcag ccaggcttcg tcacagttgc aggcctggtt acaacattat ggaacggcag
     1201 aggttaatct gcagagtggt aataactttg acggtagttc actggacttc ttattaccgt
     1261 tctatgattc cgaaaacatg ctggcatttg gtcaggtcgg ggcgcgttac attgactccc
     1321 gctttacggc aaatttaggt gctggccagc gttttttcct tcctgaaaat atgttgggct
     1381 ataacgtctt cattgatcag gatttttctg gtgataatac ccgtttaggt attggtggcg
     1441 aatactggcg agactatttc aaaagtagcg ttaacggcta tttccgcatg agcggctggc
     1501 atgagtcata caataagaaa gactatgatg agcgcccggc aaatggtttt gatatccgct
     1561 ttaatggcta tttaccatca tatccggcat taggcgccaa actgatgtac gaacagtatt
     1621 atggtgataa tgttgctttg tttaattccg ataagttgca gtcgaatcct ggcgcggcga
     1681 ccgttggtgt aaactacact ccgattcctc tggtgacgat ggggatcgat taccgtcatg
     1741 gtacgggtaa tgaaaatgat ctcctttact caatgcagtt ccgttatcag tttgataaac
     1801 cgtggtctca gcaaatcgag ccacagtatg ttaacgagtt aagaacatta tcgggcagcc
     1861 gttacgatct ggttcagcgt aataacaata ttattctgga gtacaaaaag caggatattc
     1921 tttctctgaa tattccgcat gatattaatg gtactgaaca cagtacgcag aagattcaat
     1981 tgatcgttaa gagcaaatat ggtctggatc gtatcgtctg ggatgatagt gcattacgca
     2041 gtcagggcgg tcagattcag catagcggaa gccaaagcgc acaagactac caggctattt
     2101 tgcctgctta tgtgcaaggt ggcagcaata tttataaagt gaccgctcgc gcctatgacc
     2161 gaaatggtaa tagttctaat aatgtacagc tcactattac cgttttaccg aatgggcagg
     2221 ttgtggacca ggttggggta acggacttta cggctgataa gacatcggct aaagcggata
     2281 acgttgatac cattacttat accgcgacgg ttaaaaagaa tggtgtagct caggctaatg
     2341 cccctgtaac atttagtatt gtatccggga ctgcaactct cggggcaaat agtgccaaaa
     2401 cggatggtaa cggtaaggca accgtaacgt tgaagtcggg tacgccaggg caggtcgtcg
     2461 tgtctgctaa aaccgcggag atgacttcgc cacttaatgc cagtgcggtt atatttgttg
     2521 atcaaaccaa ggccagcatt actgagatta aggctgataa aacaacagcg aaggcaaatg
     2581 gttctgatgc gattacctat attgttaaag taatgaagaa taaccaacca gaagcaaacc
     2641 attctgttac attctcaacg aactttggta atctgggggg aaattctaat acccaaattg
     2701 tgaaaacgga taaagatggt agggctacgg taaaactgac atctggcgtt gcaggtaatg
     2761 ctgttgttag tgcaaaagtc agcgaagtta atacagaggt taaggctcct gaggtaaaat
     2821 tcttctcagt tctgagcatt gatagtaatg tgagtattat tggaacctcc gctaatggcg
     2881 ctttacctaa tatttggttg cgatatggtc agtttaagct gacagccaaa ggtggcgatg
     2941 ggaaatatca atggcgctct caagatccaa gtgttgcatc agttgatgct ttaactggtc
     3001 gagttacttt gctgaagaaa ggaacaacaa caattgaagt tgtatcgggt gataaccaaa
     3061 cagcaatgta tacaattaat acacctacaa aatttatatc tgtggagaca caaaataaag
     3121 tagtctatag tgatgctgag gcaacatgta gaatgaataa tgcacgcttg ccgtcatcta
     3181 cgagtgagct aaaggatgtg tataataaat ggggcgccgc caatagttat gaaggctata
     3241 aaggtaaaaa aacaataaca gcatggacac agcaaaatga ggatgataaa caaaaaggtt
     3301 ggactagtac atttgacata gttacaaaaa atgaaatccc tagtaatggc agtaatagta
     3361 aagtccacgt gaataaagct aacgcttttg ccgtctgtgt aagatgatct tataatcatt
     3421 aattaatgcc caactaagat agttatcttg gttgggctta atagatattt attcaacctt
     3481 aatagttgta ccttatttag gtaaatttct ttacggcgag gccattattg aattgcccaa
     3541 ctgcgcttgt ttaatattct gtataaatgc ttctctcaga actgaccata tttttccacc
     3601 gctccctatt agaaaatttg ctattacttc accctacaat aaataaaaaa ccctccgaag
     3661 agggggaaga cgataataaa ttaatacgat agcggaatat gtatatattc gtctgggcgg
     3721 gagatgttga taccatcttt aggatcaatc gcgtctatga catagcctcc tttcagaatg
     3781 ctaccaattc ccaggcttgt accgttatca agagcaattg ttggattctt catcgtgccg
     3841 ctgattctga gtatttttcc ggggaaaata tctgctgaaa tgtacggctg aatgttctga
     3901 aatagtatct tgacgttact atttttgttc atcgcattta taatggcaag gatcttactc
     3961 tcgttctgtt gagaaacctc ccccgcaaca atgatgacat cattcttttt gaaaatattg
     4021 acatagttta ttaatttatt ctttttgaac tctgatgcca gttcattgat tttattgtta
     4081 ctgttgttat gtatttccca tccagcaacc cctggcgtat tcagtaacag attttcaact
     4141 tcgctccatt tgggtgaagg gggaatgtat cccgataaca ggaaaaatcc aggcttatta
     4201 ccttttgtaa tgatgatatc cttataaccg tattccgtta aaatatcatt aatcgcggag
     4261 atgatttggt tattgcaaat gatattgttg taataaaaaa tatttttttc tttcaggtaa
     4321 ttaaaaaatg gagttaaatc agtggttgat tcgcaacgtc cataaagcgc gacatttttc
     4381 ccatgccata ccagagtgat tgcatgaagt ttgttttctt ttagctgctg gtatacatca
     4441 gctctggtaa acgctggggg ttcagctttt ggggaaagca aaacgtaact gcctatggcg
     4501 ccagacaacg caatcgcagc accaatcacg ctagccagga taagacgctt tttacgtcga
     4561 tttttggcag agaacctttc tggcacatca gacaatggca aggtgtggtc aatatcacca
     4621 atgatgaagc aacatcccgc tatatcgatg acctttccga caggtaatga ctgatctgct
     4681 tcataagggg tgggctgtcc atcaatccaa aattcaaccg gagataaaag gaaaacacct
     4741 tcctcacgta tatcaagtaa aaactgattg aggccttgtt caaggggaaa ataaacaata
     4801 tcgttatctt ctgtgcctat cgttaggttt cccatcggaa gttgtaattc ccgattacgc
     4861 atcgcaccat tgagaagttt tattttatat gaggataaca tttttaaatt ttattcatcc
     4921 tggtggttga acaatgagaa aaaatatggt gaacttacat caactaagaa tagagtagaa
     4981 aggaagctgt tgtacagtac tatttaatgg aata
//
LOCUS       DAZFYC010000212         5000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFYC010000212 DAZFYC010000000
VERSION     DAZFYC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.217"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..1219
                     /gene="livK"
                     /locus_tag="ACHKW5_004980"
     CDS             110..1219
                     /gene="livK"
                     /locus_tag="ACHKW5_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO7514178.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1267..2193
                     /gene="livH"
                     /locus_tag="ACHKW5_004981"
     CDS             1267..2193
                     /gene="livH"
                     /locus_tag="ACHKW5_004981"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO7514179.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFILYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2190..3467
                     /gene="livM"
                     /locus_tag="ACHKW5_004982"
     CDS             2190..3467
                     /gene="livM"
                     /locus_tag="ACHKW5_004982"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO7514180.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3464..4231
                     /gene="livG"
                     /locus_tag="ACHKW5_004983"
     CDS             3464..4231
                     /gene="livG"
                     /locus_tag="ACHKW5_004983"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO7514181.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4233..4946
                     /gene="livF"
                     /locus_tag="ACHKW5_004984"
                     /pseudo
     CDS             4233..4946
                     /gene="livF"
                     /locus_tag="ACHKW5_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
BASE COUNT         1059 a         1276 c         1485 g         1180 t
ORIGIN      
        1 aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt ctgatttgtt
       61 aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta tgaaacggaa
      121 tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg ctatggctga
      181 cgatattaaa gttgccgttg tcggcgcgat gtccggcccg attgcccagt ggggcgatat
      241 ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg gaattaaagg
      301 cgataagctg gtgggcgtgg aatatgacga cgcctgcgac ccgaaacaag ccgttgcggt
      361 cgccaacaaa atcgttaacg acggcattaa atacgttatt ggtcatctgt gttcttcttc
      421 tacccaacct gcgtcagata tctacgaaga cgaaggtatt ctgatgatct cgccgggagc
      481 gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg ccgggctgga
      541 ctcttcccag gggcctacgg cggcaaaata cattcttgag acggtgaagc cccagcgcat
      601 cgccatcatt cacgacaaac aacagtatgg cgaagggctg gcgcgttcgg tgcaggacgg
      661 gctaaaagcg gctaacgcca acgtcgtctt cttcgacggt attaccgccg gggagaaaga
      721 tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt actacggcgg
      781 ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc tgaaaactca
      841 gtttatgggg ccggaaggtg tgggtaacgc atcattgtcg aatattgccg gtgatgctgc
      901 cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag gatccggcaa accagggcat
      961 cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct ggatcaccta
     1021 cgcggcggtg caatctctgg cgactgccct tgagcgtact ggcagcgatg agccgctggc
     1081 gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc tgaactggga
     1141 tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg ccgacggttc
     1201 atccacggca gccaagtgat catcctaccg cccgtaaaat gcgggcgggt ttagaaaggt
     1261 taccttatgt ctgagcagtt tttgtatttc ttgcagcaga tgtttaacgg cgtcacgctg
     1321 ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat tatcggcatg
     1381 atcaacttcg cccacggcga ggtttatatg attggcagct acgtctcatt tatgatcatc
     1441 gccgcgctga tgatgatggg cattgatacc ggctggctgc tggtggcggc gggattcgtc
     1501 ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtagc ctatcgcccg
     1561 gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggcatgtc catcttcctg
     1621 caaaactacg ttagcctgac cgaaggttcg cgtgacgtgg cgctgcccag tctgtttaac
     1681 gggcagtggg tggtgggcca tagcgaaaac ttctctgcct ctattactac catgcaggcg
     1741 gtgatttgga ttgttacctt cctcgccatg ctggcgctga cgattttcat tctctattcc
     1801 cgtatggggc gcgcctgtcg tgcctgcgcg gaagatctga aaatggcgag cctgcttggc
     1861 atcaacaccg accgggtgat tgcgctgacc tttgtgattg gcgcggcgat ggcggcggtg
     1921 gcgggtgtgc tgctcggtca gttctacggt gtgattaacc cctacatcgg ctttatggcc
     1981 gggatgaaag cctttaccgc ggcggtgctc ggcgggattg gcagcattcc gggggcgatg
     2041 attggcgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct gagtacggaa
     2101 tataaagatg tggtctcatt tgccctgctg atcctggtac tgctggtgat gccgaccggt
     2161 attctggggc gcccggaggt agagaaagta tgaaaccgat gcatattgca atggcgctgc
     2221 tctctgccgc gatgttcttt atcctcgctg gcgtctttat gggcgtacag ctggagctgg
     2281 acggcaccaa actggtggtc gacacggctt cggatgtccg ttggcagtgg gtgtttatcg
     2341 gcacggcggt ggtctttttc ttccagattt tgcgacctgc tttccagaaa gggttgaaaa
     2401 gcgtttccgg gccgaagttt attctgcccg cgattgatgg ctccacggtg aagcagaaac
     2461 tgttcctcgt ggcgctgttg gtgcttgcgg tggcgtggcc gtttatggtt tcacgcggga
     2521 cggtggatat cgccaccctg accatgatct acattatcct cggcctcggg ctgaacgtgg
     2581 tggtagggct ttccggcctg ctggtgctgg ggtacggcgg tttttacgcc atcggcgctt
     2641 acacttttgc gctgctcaat cactattacg gcttgggctt ctggacctgt ctgccgattg
     2701 ccgggttaat ggcagcggcg gcgggcttcc tgctcggctt tccggtgctg cgtctgcgtg
     2761 gtgattatct ggcgatcgtt accctcggct tcggcgaaat tgtgcgcata ttgctgctca
     2821 ataacaccga aattaccggc ggcccgaacg gaatcagtca gatcccgaaa ccgactttct
     2881 tcggccttga gttcagccgt accgcccgtg aaggcggttg ggacacgttc agtaatttct
     2941 ttggcctgaa atacgatccc tccgatcgcg tcatcttcct gtatctggtg gcgttgctgc
     3001 tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg gggcgtgcgt
     3061 gggaagcgtt gcgtgaagat gagatcgcct gccgttcgct gggcttaagc ccgcgtcgta
     3121 tcaagctgac cgcttttacc atcagtgccg cgtttgccgg ttttgccgga acgctgtttg
     3181 ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg gcgtttgtac
     3241 tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg gcggcaattt
     3301 tgctggtggt gtcgcgcgag ttgatgcgtg atttcaacga atacagcatg ttaatgctcg
     3361 gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc atgacgcgcc
     3421 cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc agccattatt
     3481 atctgtgaac ggcctgatga tgcgcttcgg cggcctgctg gcggtgaaca acgtcaatct
     3541 tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg gaaaaaccac
     3601 ggtttttaac tgcctgaccg gtttctacaa acccaccggc ggcaccattt tactgcgcga
     3661 tcagcatctt gaaggtttac cggggcagca aattgcccgc atgggcgtgg tgcgcacctt
     3721 ccagcatgtg cgtctgttcc gtgaaatgac ggtaattgaa aacctgctgg tggcgcagca
     3781 tcagcaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt tccgtcgcgc
     3841 ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt tgctggaaca
     3901 cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg agattgcccg
     3961 ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag gtcttaaccc
     4021 aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc acaacaccac
     4081 tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc gaatttatgt
     4141 ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta ataacccgga
     4201 cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg tcctttgaca
     4261 aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg catatcaatc
     4321 agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc ttgctcggca
     4381 cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat aaagacatta
     4441 ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg gaagggcgtc
     4501 gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt tttgccgaac
     4561 gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gtttccacgt ctgcatgagc
     4621 gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg gcgattggtc
     4681 gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctc ggtcttgcgc
     4741 cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag gggatgacta
     4801 tcttcctcgt cgagcagaac gccaactagg cgctaaagct ggcggatcgc ggctacgtgc
     4861 tggaaaacgg ccatgtggtg ctctccgata ccggcgacgc gctgctggcg aacgaagcag
     4921 tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc aaaagactcc
     4981 tttgagacag gtgacaaatg
//
LOCUS       DAZFYC010000213         4971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFYC010000213 DAZFYC010000000
VERSION     DAZFYC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.218"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1241
                     /locus_tag="ACHKW5_004985"
     CDS             <1..1241
                     /locus_tag="ACHKW5_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFO7514182.1"
                     /translation="YKPEKPSDTELVFISNAETIRDYLLRLSVDELKLLAKYILQNVY
                     IVFVQTDDFASSFRLFNVLNSRGLPLSNADLLKNALFESASTHNKKSEQIESAWSQIE
                     DMVGVRRLDKFLTLHKLSEKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAK
                     NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITA
                     FEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDED
                     LYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQ
                     EHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGL
                     AELKMRHEKMKTQLKKLWLV"
     gene            complement(1310..2266)
                     /gene="yjiA"
                     /locus_tag="ACHKW5_004986"
     CDS             complement(1310..2266)
                     /gene="yjiA"
                     /locus_tag="ACHKW5_004986"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7514183.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEVLCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLHERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(2277..2480)
                     /locus_tag="ACHKW5_004987"
     CDS             complement(2277..2480)
                     /locus_tag="ACHKW5_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7514184.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2611..4761)
                     /gene="btsT"
                     /locus_tag="ACHKW5_004988"
     CDS             complement(2611..4761)
                     /gene="btsT"
                     /locus_tag="ACHKW5_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO7514185.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPLIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
BASE COUNT         1321 a         1193 c         1263 g         1194 t
ORIGIN      
        1 attataaacc tgaaaagcca tcggatactg agttagtatt cattagcaac gctgaaacaa
       61 ttagagatta tttgttgcgg ttatctgttg atgagttgaa gttgcttgct aaatatattt
      121 tgcaaaatgt atatatcgta tttgtgcaaa ctgatgattt cgcatcatca tttagactct
      181 ttaatgtatt aaatagtcga ggtttacctt taagtaatgc agatctttta aaaaatgcac
      241 tgtttgaatc ggcttctacg cataataaaa aatcagagca aatagaatca gcatggtcgc
      301 aaattgaaga tatggttgga gtgagacggc ttgataaatt tcttactctt cataagcttt
      361 ccgagaaaaa agatcgtgac agggtgttac aaaaaggttt tgaagctttt attgaaaatc
      421 tccagcagca atttgatgga gatgctattg ctatgtcact gatgttggtt aattctgcaa
      481 aaaattatac taagatttta gagaatgatt ttgaacaccc ttctatacga cgaaaaatag
      541 catcgctaag taatttaggg gtagatgagt ggattcctcc ggttatggca ttcatgaatc
      601 gaatggctcg tacagaagat tttaatttag atgatttttc tcaatttatt accgcctttg
      661 agaaggtata tatgcatggg tggttaaaaa aacaaattaa aagccaaaga gaaatggttt
      721 gttattcagc tctggtagca ataaataatg atatgccttt tgattctgtg ataaatcaga
      781 taaaccaaca tgcagataat tcaggtttta tagctgctct ggatgaagat ttatatgagc
      841 ctcgtccgaa tcaagtgaac ttaataaaag caattttgct tcgcttagat atggagcaac
      901 aagatgagag cgtgattaaa acctatacgg ggagaatcac aattgagcat attcttcctc
      961 aagcattagt taatgaatac tggattaata gattccaacc tcaagagcat gtctactggc
     1021 tgcataaaat aggtaattta acactcataa gtggttccaa aaattctgaa gcacaacact
     1081 atgattttat caaaaagaaa tctatttatg agaaacttaa tagtaaaagt tcttttgatt
     1141 taacaaaaga tgtttgtaat tcttcagaat ggggattggc tgaactgaaa atgagacatg
     1201 aaaaaatgaa aacgcaattg aagaagcttt ggttggtctg acatatctaa agagttccac
     1261 tcgtaataat gttgcggtgg aactctcaaa aagttgatct ttcccgaact tacttcctca
     1321 accccgcaaa cgcagcccga atctcttcct ccggcagctg gatcccgata aacaccatcg
     1381 tgctatgcgg tttttcatcg ccccacggcc tgtcccagtc ggcgctgtag aggcgctgga
     1441 cgccctggaa cagcaggcgg ttaggttcgc cgtcaatcca cagcatccct ttgtaacgta
     1501 gcagtttatc cgccgactcc agcagcaggt tttccatcac gcgggaaact tcgctgatat
     1561 ctaccgggta atccagttcc accacaatcg acgaaatatc gttttgttta tctgcgataa
     1621 agtggaaacg cggtttggtg ctgacgacgt tttcttccag cataaaaccg ttggtgttga
     1681 acagcaggcc cagatcgatg tcgccgtggg tgacggtgta gaccggtgcg cgggcgttga
     1741 tgcgcgccag gcgttcatgc agtttttctg cttcgcctgc gacgtcggtt ttggtcagca
     1801 gaatgcggtc ggcgtagcca acctgtgact gggcgatggt gaactggttc atctgctcat
     1861 cggcatgtac cgcatccacc agcgcaatca cgccgtccag cagataacgc tggcataaaa
     1921 cttcatggga gaaaaaggtc tgaataatcg ggccgggatc ggccatgccg gtgcattcaa
     1981 tgaccagacg gtcgaactga atattgccct tgtcgagatt gtccagcagg tcgagtagcg
     2041 cgtcctccag ctcgttggag cgcgaacaac agatgcagcc gttggtcagc gttttgatct
     2101 gcgtggcgcg gtcgccaatc agttgatcat caacagagac ttcgccgaat tcgttttcaa
     2161 tcacggcaat tttgtagccg tgctgttcat taagaatatg gcgcagcagg gtggttttgc
     2221 ctgcgcctaa aaaaccggtg agtagggtaa ctgcaatcgg gttcatgctc tctccattaa
     2281 caacagcgca taccgccttt accatcgccg ccgtagcgcg cgttctggcg ttcgcggaag
     2341 aattcttcat agctcatgta cggcttgtcg ggatggttgg tcttcatatg ctcgacgtag
     2401 ttgtcgtagt ctggaatgcc aatcaacatc ttcgccgcct ggccgagata tttttttgcc
     2461 tgtcctaagt taccaaacat tttgccttcc agatatgcgt aaagcccgga aggtccgggc
     2521 cggggatttg ccggatgcgg cgtaaacgcc ttatccggcc tacgatagac actttgtagg
     2581 ccggatacgc gcagcgcatc cggcaaccgg ttagtggtgc gaagagatct tcacgccgcc
     2641 ttccgggatt ggaacgtacg gtgtttcttt gtcagtacgt ttgtcgctgt ttcgcacctt
     2701 cagccaggtt ttgaaaccgt agaagatgat gctgtacacc acaatcagga acagaatact
     2761 cagacccgcg ttagtgtagt tgttcacaac gatgtggttc atgttggcaa tctgctgcgc
     2821 cgtcaggtca gtaccgttag caatcttctc tttgtactgg cttgccatgt agaagaagcc
     2881 ttccatctgc gggttggtgc tgaacagttt cagacccagc gcccaggtgg tgcagataag
     2941 cagccataca gccggaacaa cagttaccca gatgtattgg gtgcgcttca tcttaatcag
     3001 cacaacggtg cccagtacca gcgctacggc tgccagcatc tggttggaga taccgaacag
     3061 cggccacagg ctcttaacgc cgcccagcgg atcgaccacg ccctgataca gcaggtagcc
     3121 ccacagaccc acacagcccg cagtaccgat gataccggca accagagagt cggttttttt
     3181 caggaacggg atgaagttac ccagcaggtc ttgcagcata aagcggccag aacgggtacc
     3241 cgcatccagc gcggtcagga tgaacagggc ttcgaacaga ataccgaagt gataccagaa
     3301 gcccatgtca gccatcggca gcactttgtg gaacacatga gcgataccta ccgccagcgt
     3361 cggcgcacca cctgcacggt tcaggacaga aggctcacca atgtctttcg cggtttgcag
     3421 gatctgctct ggcgaaatca cgaagcccca ggagctgacg gtcgccgccg cgtgtgcggt
     3481 aacgtctttc agctgcgcca tgatgagcgg cgcgttctcg ccacccattt catgcaggtt
     3541 aggcatggtg atgccaaggc ccgcaggcgg ggtattcatc gcgaagtaaa gacccggttc
     3601 gatgatggac gcagcaacca gcgccataat cgccacgaag gactccatca gcattgcgcc
     3661 gtagccaatg aaacgcgcgt cggtttcgtt agccagcagt ttcggcgtcg taccggaaga
     3721 gatcagcgcg tggaagccag ataccgcacc acaggcgatg gtgatgaaca ggaacgggaa
     3781 cagagcgcct ttccacagcg ggccagtacc gtcaatgtac tgggtcatcg caggcatttt
     3841 cagttccggg ttcagcacca cgatacccag cgccaggccg acgataacgc cgattttcag
     3901 gaaggttgcc agatagtcgc gcggtgcgag gatcagccac accggcagca gtgcggaaac
     3961 aaacgcatag ccaatcagcg cgaaggtaat ggtggtgtct ttaaaggtca gtgccggacc
     4021 ccagtacgga tcgtgagcaa tcacgccacc gaagtagata gaggcaacca gcagcacgat
     4081 accaatgaca gagacttcac ccacacgccc cggacggata aagcgcatgt agatacccat
     4141 aaacagcgca atcggtacgg ttgagcaaac ggtgaagaca ccccacggac tttcggccag
     4201 ggctttaacc acaatcagcg ccaggacggc gaggatgatg atcatgatta agaaacagcc
     4261 aaacagcgcg atagtccccg gtactggtcc catctcttct ttgatcatct caccaagaga
     4321 tgcgccatta cggcgagagg agataaacag caccataaag tcctgaaccg caccggccag
     4381 cacgaccccc gccagcagcc acagcgtgcc aggcaggtag cccatctgcg cggcgagaac
     4441 cggacccacc agcggaccag caccggcgat agcggcgaag tggtgaccaa acaacacgta
     4501 acggttggtc ggaacgtagt tcagaccgtc gttgttaata accgcaggcg tcgcgcgcgt
     4561 ggggtcgagt ttcatcacct tctgggcgat gtacagactg tagtagcgat acgccaccag
     4621 atacaccgat acagaggcga ccacgatcca cagggcgctg acgtgctccc cccgacgtaa
     4681 tgcaactacc gcgaggcaga atgcaccgat gattccgaga atcacccagg gtatgtgctt
     4741 gaatatcttt ttagtatcca tagtaaaacc tggcatgtat tgattaatag ttggccgaag
     4801 ccgttctagg tttgcgttgc gtttgaggag gtaaattgac cgctatcctg tagtgatgtt
     4861 gccagagtta cgcgcgcgta aagtaaggta aataactgag tggttatttt agggatgtaa
     4921 gcggtcagtt ttgcggttga gcggcaaagg cgtcgtttaa gtgacgataa a
//
LOCUS       DAZFYC010000214         4928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFYC010000214 DAZFYC010000000
VERSION     DAZFYC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.219"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            158..973
                     /gene="cma"
                     /locus_tag="ACHKW5_004989"
     CDS             158..973
                     /gene="cma"
                     /locus_tag="ACHKW5_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HFO7514186.1"
                     /translation="METLTVHAPSPSTNLPSYGNGAFSLSAPHVPGAGPLLVQVVYSF
                     FQSPNMCLQALTQLEDYIKKHGASNPLTLQIISTNIGYFCNADRNLVLHPGISVYDAY
                     HFAKPAPSQYDYRSMNMKQMSGNVTTPIVALAHYLWGNGAERSVNIANIGLKISPMKI
                     NQIKDIIKSGVVGTFPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANGSWTYN
                     GVVRSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
     gene            complement(1023..1376)
                     /gene="cmi"
                     /locus_tag="ACHKW5_004990"
     CDS             complement(1023..1376)
                     /gene="cmi"
                     /locus_tag="ACHKW5_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO7514187.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GMYHCEEGYVEYAND"
     gene            complement(1553..2329)
                     /locus_tag="ACHKW5_004991"
     CDS             complement(1553..2329)
                     /locus_tag="ACHKW5_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7514188.1"
                     /translation="MQYLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNR"
     gene            complement(2331..2744)
                     /locus_tag="ACHKW5_004992"
     CDS             complement(2331..2744)
                     /locus_tag="ACHKW5_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514189.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLDGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            3021..3284
                     /locus_tag="ACHKW5_004993"
     CDS             3021..3284
                     /locus_tag="ACHKW5_004993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514190.1"
                     /translation="MTQHIVSSAAKSGTTCCADGRWRCVGERKIRDHQKPDLRDEKRV
                     VDMILYKKHKTIPGVMFVIIYKFIPDMIFTKVEINYSLYDFTY"
     gene            3312..3581
                     /locus_tag="ACHKW5_004994"
     CDS             3312..3581
                     /locus_tag="ACHKW5_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514191.1"
                     /translation="MDEKVYFRLSYETMTGDTEDFINACIERANRADCNDTDAEITRA
                     RSTIELWYCLALAGRAPDDVADRDHLRFTEMLLRAPTAEQRACQL"
     gene            3569..4165
                     /locus_tag="ACHKW5_004995"
     CDS             3569..4165
                     /locus_tag="ACHKW5_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514192.1"
                     /translation="MSAVTSRGPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSG
                     EAPVYGPNLRASCRRMEASGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAK
                     QRAAEVVVLPLVPASGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNR
                     EGKVVRREGDPLEVAQWLQACHDAGIKVRVQVNESITP"
     gene            complement(4183..4530)
                     /locus_tag="ACHKW5_004996"
     CDS             complement(4183..4530)
                     /locus_tag="ACHKW5_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO7514193.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            4641..>4928
                     /locus_tag="ACHKW5_004997"
     CDS             4641..>4928
                     /locus_tag="ACHKW5_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO7514194.1"
                     /translation="MKADISVKYFLKSLIPGFIILTVFYLGWKDNQENSRMFYAFIGC
                     IISSITFPFSMKIIQKMVIRFTGKEVWQKDFFTNPVGGSLTVISELFCFVIS"
BASE COUNT         1273 a         1262 c         1159 g         1234 t
ORIGIN      
        1 tatcttatat atggtagttc aggtgcttcc ctaccatttt taacggcata tccggcaaac
       61 aatgttatgg ggagcctctc tcatggggtt cgttcgccgg tgtaagcgtt agccagccgc
      121 gaactctgtt atcttattaa cttataagga gttatgtatg gaaaccttaa ctgttcatgc
      181 accatcacca tcaactaact taccaagtta tggcaatggt gcattttctc tttcagcacc
      241 acatgtgcct ggtgctggcc ctcttttagt ccaggttgtt tatagttttt tccagagtcc
      301 aaacatgtgt cttcaggctt taactcaact tgaggattac atcaaaaaac atggggccag
      361 caaccctctc acattgcaga tcatatcgac aaatattggt tacttctgta acgccgaccg
      421 aaatctggtt cttcaccctg gaataagcgt ttatgacgct taccacttcg caaaaccagc
      481 gccaagtcaa tatgactatc gctcaatgaa tatgaaacaa atgagcggta atgtcactac
      541 accaattgtg gcgcttgctc actatttatg gggtaatggc gctgaaagga gcgttaatat
      601 cgccaacatt ggtcttaaaa tttcccctat gaaaattaat cagataaaag acattataaa
      661 atctggtgta gtaggtacat tccctgtttc tacaaagttc acacatgcca ctggtgatta
      721 taatgttatt accggtgcat atcttggtaa tatcacactg aaaacagaag gtactttaac
      781 tatctctgcc aatggctcct ggacttacaa tggcgttgtt cgttcatatg atgataaata
      841 cgattttaac gccagcactc accgtggcat tatcggagag tcgctcacaa ggctcggggc
      901 gatgttttct ggtaaagagt accagatact gcttcctggt gaaattcaca ttaaagaaag
      961 tggtaagcga taatataagg gccggggttc cggccttttt atgcagcctt tataacagtg
     1021 tattagtcat tcgcatattc aacatatccc tcctcacaat ggtacatgcc ataatacatt
     1081 ctcttaccct ccgggccttc agctttaaat gggacaagaa gtgttttttc atcttcaaca
     1141 tctgttaaac ttctctcaat cttttcccat gtaattttcg ggatttctgt accaagaagt
     1201 tgtacatcgt tgtcccagcg tttcattctt tgcaggtaat cattttttac aaacgttctg
     1261 gcctgttcat cgctgacctg atagcatgcc ggacctttat cttcagacca gaaaaaaaca
     1321 gcagcaagag caataatcgt taaaataaaa ataaatttca tgctaattac tttcataaaa
     1381 tctccacaac ttctgtcgtt cattctatat aaaaacacat tacgaatata tccgtacaat
     1441 atgagcatac tgtgaagtgg ctttaacaat tatgaaataa tacaagagat cacatattgc
     1501 agtggtctct ttttttacag aataaatcac aattcataat tatgatttat gcctacctgt
     1561 ttttcagcat tgtgacagca tcggactcag gcatcgaaaa ctgcacccgg tgccgtgcag
     1621 ccacatccag tgcaaacacc ttcgtgtaga cctccgttga gctgatggac ttatgcccca
     1681 tcagactctg caggaccttc agcggtatac cggcatacag catatgcatc gcataggaat
     1741 gacggaacgt atgtggcgtg accgggacag agaacgtcac tccatcagcg gcagcggcgg
     1801 caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag atccgcgctt
     1861 tctctgtcct gcctgttcgt ttattacgcc gttccatggg gattttcagt gtggccacca
     1921 tcgtctgcag ctggctgacg taccaggcgt cggagagcgg aaccagccgg tgagtctgct
     1981 gaccggcggg cattcttcct gccgtcctgg cggctttttc ggtccgctgc ttcagagtgg
     2041 ccagctgcac aaacggatac gggggcgtga gcgaaaaatc cccccgcgtc agcgccagtg
     2101 cttcattaat gcgcgcaccg gtgttccaca gtgtggccag cagcatcttg cggcgcagat
     2161 ccgggacgta atggagcagg gcgctcactt caggggccag caggtatttt ggcagttcat
     2221 catgaaccat cgacatctgg cggagtgcca gcgctgccgg ataatcgatg gcaacaggaa
     2281 gctgagcggc atcccgggca tggtggatcg gtgccggcag gtactgcata tcagtgttca
     2341 ctccacgggg aaacgtcggg caggttttcc acaacacggt actttcccgg ataacggagc
     2401 tggttaccgc cacgcgtcca gacggtgaca acaaaacccc cgccctggtt gttttcgggg
     2461 agcaggcgat cgcactgctc gctcagcatg tggatcacga acggcgcatc gctgccgtct
     2521 tcaaatacca gttccagcgc atcgcggcca tccagcgttc ccttactgat gatgacgtac
     2581 tcagcccccc gcatctccct gagtaaatgt tttgccgcat ccgggacaag cagcctggct
     2641 gcgccggcat tccagctgag gtagacatat ccggcctgcg cctgtacaga ctgccagtaa
     2701 tccgttccgg cgacagcctg accttcattc tcaataaaaa acatgggtga ctcctttttt
     2761 cttttccggg cagggacggt catgcgataa cgtcatccat catcactgcc cttaccgtca
     2821 cgccgtctga taacatcatg ccgttatcat catcaccatg ataacaagca tccatataac
     2881 cacgccgtta aattaccgga gagccgtttc cgtcagatcc gactttccgg aatatcacaa
     2941 ctttccggaa gtgtaccacg ctggggaaat aatcatatcg ctgttttccg cccctgcgtg
     3001 acgcatcaca ggtcagggag atgacacaac atatagtgtc atcagcagcc aaatccggca
     3061 ccacatgttg tgctgacggg aggtggcgtt gtgtggggga gaggaagatc cgtgaccacc
     3121 agaaaccgga tctgcgtgat gagaaaagag tagtagatat gattttatat aaaaaacata
     3181 aaaccatacc aggcgtgatg tttgtcataa tatataaatt catacctgat atgatattta
     3241 ccaaagtaga gataaattat tcgctgtatg attttaccta ttaatgcaca taatcaaatg
     3301 gagcatgcag gatggatgaa aaagtatact ttcgcctcag ctatgaaacg atgaccggcg
     3361 atacggaaga cttcattaac gcatgtattg aacgggcaaa ccgtgcggac tgcaacgata
     3421 ccgatgcgga gattacccgc gcacgcagca ccattgaact ctggtactgt ctggcactcg
     3481 cggggcgtgc cccagacgac gtcgccgaca gggatcatct gcggttcacc gaaatgctcc
     3541 tgcgcgcccc gacagcagaa caacgcgcat gtcagctgta acctcccgtg ggccgtcgcc
     3601 gctgcaacga cgggtgctga ttgtgctggc tgcccttgat gaaaaacggc ctggccccgt
     3661 gctgactcgg gacatagaac gggtgctgga gcgaagcggg gaagccccgg tctacggacc
     3721 aaacctgcgc gcctcctgcc ggcgcatgga agcatcaggc tggcttcgca cactgcgcgc
     3781 cccgaatctg cagctggctg tcgaactgac ggacgccggc cgggccgtgg cacagcctct
     3841 gctgctggca gaacaggacc gcctccgggc aaaacagcgc gcggcagaag tcgtcgtact
     3901 gccgctggtc ccggcatcag gtctgcccgc agacggcact tctgccactg atctggcggt
     3961 ggagctgaac ggcatgacgt atcaggcctg ccgtggggat tttgtggtac gtctcgacgg
     4021 cagcacctgt ctgcagttat ggaacagaga aggcaaggtg gtacgccggg agggcgatcc
     4081 gctggaagtg gcgcagtggc tgcaggcctg tcatgatgcc ggaataaaag ttcgtgtaca
     4141 ggttaatgaa agtatcaccc cgtaaaaaca tgcctgcacg attcacacat catcccatgg
     4201 tggcagccgg ttaactttcc tgcgccacca gtggaaagag gccatgagca gtccgaacaa
     4261 aaaaccaccc gtgatactta taataatggc ctcaccggcc accattcctg acggccccca
     4321 gtagataaac cacattgcac atccccagga aatgccccat aagccccccg tcagcaccgt
     4381 gacctgccag aacggcataa agggtaatgg cggaagccgg atccccagcc gccagagaat
     4441 acgcagaaga ggaggggcat aattgctccg ccacatcttt ttgctgtcca tcagggcaat
     4501 ggcccgggct tttttctgct caaaagtcac aggagtacct ccgttccatg atgtaaacgt
     4561 gacacagttt attgttattg attattatta tcattatgtt atcacgcacc agtacacgat
     4621 gacaataaag gagaaaccag atgaaagcgg atatatctgt aaagtatttt ctgaaaagcc
     4681 tgataccagg tttcattatt cttacagttt tttatctggg atggaaagat aaccaggaaa
     4741 attcaagaat gttttatgcg ttcatcggat gcattatcag ctccattact tttccttttt
     4801 caatgaagat aatacagaaa atggtaataa ggtttacagg gaaagaagtc tggcaaaaag
     4861 acttctttac taatccagtt ggcggaagct taactgtaat atctgaatta ttctgtttcg
     4921 ttatatca
//
LOCUS       DAZFYC010000215         4875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFYC010000215 DAZFYC010000000
VERSION     DAZFYC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.220"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            202..278
                     /locus_tag="ACHKW5_004998"
     tRNA            202..278
                     /locus_tag="ACHKW5_004998"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Val,seq:gac)
     gene            283..359
                     /locus_tag="ACHKW5_004999"
     tRNA            283..359
                     /locus_tag="ACHKW5_004999"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:317..319,aa:Val,seq:gac)
     gene            467..772
                     /locus_tag="ACHKW5_005000"
     CDS             467..772
                     /locus_tag="ACHKW5_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO7514195.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            898..2502
                     /locus_tag="ACHKW5_005001"
     CDS             898..2502
                     /locus_tag="ACHKW5_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO7514196.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(2514..3326)
                     /gene="ydhT"
                     /locus_tag="ACHKW5_005002"
     CDS             complement(2514..3326)
                     /gene="ydhT"
                     /locus_tag="ACHKW5_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO7514197.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(3330..4115)
                     /gene="phsC"
                     /locus_tag="ACHKW5_005003"
     CDS             complement(3330..4115)
                     /gene="phsC"
                     /locus_tag="ACHKW5_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO7514198.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(4112..4780)
                     /locus_tag="ACHKW5_005004"
     CDS             complement(4112..4780)
                     /locus_tag="ACHKW5_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7514199.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
BASE COUNT         1299 a         1240 c         1120 g         1216 t
ORIGIN      
        1 aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat
       61 cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa
      121 tagcgcagaa aattacgttt tgcctcttgc caccttccca ctctgccgct aatattcgtc
      181 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
      241 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
      301 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
      361 attttcttaa tctggtcttc tcctttttcc ctctgtttct tctctgtatc caatacgtta
      421 aaagatttac actgtcttcg tatgcgttat cagaaggaga atcgctatgg caactttgtt
      481 acaacttcat tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc
      541 gcttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga
      601 aaagaaccac gaagccggtg ggatctatct ttttactgat gaaaaaagcg cccttgccta
      661 tctggaaaaa catactgccc gacttaaaaa tctcggcgtt gaggaagttg tcgccaaagt
      721 tttcgatgtc aatgagccac tgagtcaaat caatcaggca aaactcgcct gacagaattt
      781 aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc
      841 tcaaccaaac ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg
      901 aaaaaaattg ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta
      961 cagcaacaaa ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg
     1021 atgccataca gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa
     1081 ataccgccga ttaattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc
     1141 cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg
     1201 ctgggcgaat atttccgcga tcaattttta cgattagtag accaggcacg acagcaaaaa
     1261 tttgcagtgg ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc
     1321 gtcatgctcg ctacaaatca ggatttaccc agcgagacgt ttgatttagc ggtgattgcc
     1381 acgggtcacg tctggcctga tgaagaagaa gcaaccagaa cgtattttcc cagtccgtgg
     1441 tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg
     1501 agcggactgg atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat
     1561 aaacaacacg tcgtttttaa ccgcgataac gcaagtgaaa agctaaatat tacgttgatg
     1621 tcgcgcacgg gtattttacc cgaagccgat ttctattgcc ctattcccta cgagccctta
     1681 cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg
     1741 gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa
     1801 cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc
     1861 aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa
     1921 cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag
     1981 gaaattgttc ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg
     2041 gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcgcct acttgcctta
     2101 cgtgaagcgg gaatcattca tattctcgcc ctcggtgaag actacaaaat ggaaattaat
     2161 gagtcgcgca ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttattgat
     2221 gcccgcggac agcgtccgct taaagtgaaa gatattcctt tccctgggct acgcgagcaa
     2281 ttacagaaaa caggggatga aatccctgat gttggcgaag attatacgtt acagcaaccc
     2341 gaagatattc gtggacgcgt agcgttcggc gcgttgccct ggttgatgca cgaccagcct
     2401 ttcgttcagg gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta
     2461 aagcctgcat cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac
     2521 tcaacaaact gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca
     2581 ccttcaatgg cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt
     2641 ccccctggcc cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca
     2701 ccgctggcaa tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag
     2761 ctggcgattt gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa
     2821 acgcgtactc gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc
     2881 gaagaattac cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta
     2941 aagccgacac ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca
     3001 gcggttgcga ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg
     3061 gcaaactgac tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa
     3121 acttcctgat gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga
     3181 ctctccgccc agtccgtata tccttcctca atcagtgcat gatgaatatc ggcataacta
     3241 ccgccgcgtg ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct
     3301 cgcaggtctg ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt
     3361 ttaaaggttt cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa
     3421 aagaggctta taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga
     3481 aacacatctc ccacggcttg cggatagaga cacagcagcc ccgtcaatag taacaacggc
     3541 agcaatccat acataacacc aacataggcg acctgctgta agggattaaa tttagactgg
     3601 gttgttgccg ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt
     3661 tgttttgccg ctcgctccag ccacccctga cgacgaatgc gatagtggtg accattaccc
     3721 ccaacggcat tgatcagcac aaagccgagc cagcatgcga gtaacaaaaa tccgcaaact
     3781 tcatgcaccg caaccagact tttaaccgca gttgcgccca ccagcgcaaa gtgatttatc
     3841 agcccacttg ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc
     3901 gctttgcagt aaagataaat cttctcacca taatcggtga ctgatttctt cacgccacga
     3961 gcacgaagca aggcatgcag ccccagcacc aaccacatcc caaccagcaa tactccggca
     4021 atgatcaacc acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg
     4081 ctctgaaact gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt
     4141 gaccgaaccg gcgatacaga tgcggtttcc cggcaccagg tagctgatat tgatagtatt
     4201 tattttgctg caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt
     4261 gttccgggca ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc
     4321 agaaatcaca tttatctgcc actttagtga cagggttaag gtagcgaacc tggtacggac
     4381 aagcgccaat gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct
     4441 gctcatcacg ccacgacgcc cctgtcgggc atacgtcgat gcacggagca tcttcgcagt
     4501 gctgacatga ctggcgaaaa aagtgatact gcgtttcgtt atcgttatcg gtgactggaa
     4561 tgtgtgctat cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc
     4621 gggcgcaaat attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt
     4681 ccttttcttg cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg
     4741 ttcccatccc cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag
     4801 tggctgcaac tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact
     4861 gctctacctc gaacc
//
LOCUS       DAZFYC010000216         4863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFYC010000216 DAZFYC010000000
VERSION     DAZFYC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.221"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            149..1123
                     /gene="hemB"
                     /locus_tag="ACHKW5_005005"
     CDS             149..1123
                     /gene="hemB"
                     /locus_tag="ACHKW5_005005"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO7514200.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1229..2080)
                     /gene="tauD"
                     /locus_tag="ACHKW5_005006"
     CDS             complement(1229..2080)
                     /gene="tauD"
                     /locus_tag="ACHKW5_005006"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO7514201.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2077..2904)
                     /gene="tauC"
                     /locus_tag="ACHKW5_005007"
     CDS             complement(2077..2904)
                     /gene="tauC"
                     /locus_tag="ACHKW5_005007"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO7514202.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2901..3668)
                     /gene="tauB"
                     /locus_tag="ACHKW5_005008"
     CDS             complement(2901..3668)
                     /gene="tauB"
                     /locus_tag="ACHKW5_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO7514203.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3681..4643)
                     /gene="tauA"
                     /locus_tag="ACHKW5_005009"
     CDS             complement(3681..4643)
                     /gene="tauA"
                     /locus_tag="ACHKW5_005009"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7514204.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDYAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1119 a         1405 c         1244 g         1095 t
ORIGIN      
        1 aatatgaaat tattgctatt tctattacaa agatgtcctg gtctctcttt cattctcttc
       61 ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt atactgtgca
      121 ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc ctcgtcgcct
      181 gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc ttaacgacct
      241 ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg aagccatgcc
      301 aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca tcgccaacgc
      361 cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa ccggcagcga
      421 cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc agactgtgcc
      481 agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg gtcactgcgg
      541 tgtgctgtgc gagcatggcg tcgacaacga cgcaactctg gaaaatttag gcaagcaagc
      601 cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga tggacggcca
      661 ggtacaggcg attcgccagg cgctggacgc tgcgggattt aaagatacgg cgattatgtc
      721 gtattcgacc aagttcgcct cctcctttta tggcccgttc cgtgaagctg ccggaagcgc
      781 attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg aggcgattcg
      841 tgaatcactg ctggatgaag cccagggcgc agactgcctg atggttaaac ctgctggagc
      901 atacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg gcgcgtatca
      961 ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta tagatgaaga
     1021 gaaagtcgtg ctcgaaagct taggttcgat taagcgtgcg ggtgcggatc tgattttcag
     1081 ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt cctctcagaa
     1141 agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc ctctccccaa
     1201 gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt ttatccccaa
     1261 ggatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg gcatagtgct
     1321 gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa cgcacctgaa
     1381 actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc tctttctcgc
     1441 tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt ttaccgctca
     1501 ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc gcctcgcgcc
     1561 agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat ttacggaaat
     1621 catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc gcctcatagg
     1681 ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaactct ttagctgcca
     1741 gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag ttgtcgttat
     1801 ccggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct tcggcatgag
     1861 ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt tgctgctgcg
     1921 gcgtaatagt ttgatcgcgc agaaacacca cctgatggcg cagcaccgca tggtaaagct
     1981 gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt tgtgcgccga
     2041 tatacggccc cagcggggta atgctcagac gttcactcat tgtacttctc catgccaggg
     2101 cgtcagacgg cgctgtaacg cgcgcaaacc cagttctaaa agaaaggcga taatcgcaat
     2161 caccgcgatt cccgccagca ccacgtcagt cgcgagaaat tcacccgctg actgaaccat
     2221 aaatcctaaa ccgcgcgtcg cggcaatcag ctccgccgct accagcgtag accagcccac
     2281 acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca aaatgacaaa
     2341 ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc gaacctgctg
     2401 cgcgcttttc acccccgcca gcgccgacat cgccaccggt gcaaaaatcg ctaaatagat
     2461 cagtaagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg gcaaataagc
     2521 cagcggcggc acgggacgat aaagttcgat taccggatcc agaatgccgc gcaccgtcgg
     2581 gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga gtagcgccag
     2641 cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca taaatccctg
     2701 cggtccggca atggtgagca gttttgccag tacttgttgc ggcggcggca aaaacagcgg
     2761 gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg ctaacgtgcc
     2821 aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc gcgaatgcag
     2881 tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac gcggcttaaa
     2941 acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct cgactctccc
     3001 gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacacggcc agggccggat
     3061 gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg ggtaatcaac
     3121 agcacctgct tgcccgtctc ctgccagagt ttcagcagca gagtttgcat ctggtcgcgg
     3181 gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg attcgccgcc
     3241 agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca gatgtagcgt
     3301 ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc cagtcgctgc
     3361 attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac attgcgccac
     3421 ggtagtagcc cttcattctg aaaaactacg ccacgctctg ctcccggtcc ctcaatacgc
     3481 ttacccgcca gtagaatgct gccatgctga taaggcacaa aaccggcaat cagattcagc
     3541 aacgtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc gctttccagc
     3601 gtcaggttga tatcttccag tgccggtttg ccgccataat cggcgtaaag atgagagatt
     3661 tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg gctgtaatca
     3721 ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc gatgatcgct
     3781 ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata ggtattcccc
     3841 ttcaccagcc ccggaacgtc accttcaggc acgccgctta aacgcgccag tttgctgatg
     3901 ttttccggct gtttcagcca cacgtctggg ttagcaatgt acggttgctg agcatcgatg
     3961 gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcataatc tttgcgcacc
     4021 acccagacgt ccagcgttgg cgcgccccac tgcccgacct gttcagaatc ggtcaacacc
     4081 ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc atcaatatct
     4141 ccccgctgcc aggcagcgat aatcgcgggc ggctgcaggt tcacaatctc cacttgcccg
     4201 ggtttaatgc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt ggagataaac
     4261 ggtacggcga tgcgtttgcc aatcagatct tccggtttgc tgatagtttt ctttaccacc
     4321 agcgcttcgg agttacccag ttttgacgcc agcaagaaga cttcaatcgg cacctgttgg
     4381 ctggctgcaa ccgctaacgg gctggaaccg aggttgccga tttgcacatc gcctgaagcc
     4441 agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacgct cgccccgctc
     4501 tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga ggtttgatag
     4561 gccacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc ggctagaagt
     4621 gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg ggagcagtat
     4681 tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga ttttattctt
     4741 agcaaaaaaa cggctaagga aagattaaat atgcaattac tccgttctaa caattgggaa
     4801 aattactaca cttcatttta gtgatgcata aacaaccaca ttacgagatt tattgcttca
     4861 tta
//
LOCUS       DAZFYC010000217         4857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFYC010000217 DAZFYC010000000
VERSION     DAZFYC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.222"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..651
                     /locus_tag="ACHKW5_005010"
     CDS             <1..651
                     /locus_tag="ACHKW5_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO7514205.1"
                     /translation="LSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            779..1315
                     /gene="yfdR"
                     /locus_tag="ACHKW5_005011"
     CDS             779..1315
                     /gene="yfdR"
                     /locus_tag="ACHKW5_005011"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7514206.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            1306..1656
                     /locus_tag="ACHKW5_005012"
     CDS             1306..1656
                     /locus_tag="ACHKW5_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFO7514207.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKDELANEPHPIDGLSGEYAEKS
                     LEEWAEQIRKGGNQ"
     gene            1653..2297
                     /locus_tag="ACHKW5_005013"
     CDS             1653..2297
                     /locus_tag="ACHKW5_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514208.1"
                     /translation="MNNRFYMMCLRETVGNNASFHCHNGNGYSSNIDRAHVYTLEEAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTENLIEPDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADSAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLKKQNRRKKSRSGKVRWNCPHCGKITWQYNPYDFEGCSDYNCEGWRE"
     gene            2294..3466
                     /locus_tag="ACHKW5_005014"
     CDS             2294..3466
                     /locus_tag="ACHKW5_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7514209.1"
                     /translation="MTIDYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWKG
                     EPGGVIAECHVNCLIETDVQAYANAEFIAAFNPNVALALLDERERNQQYIKSRDQENE
                     DIALTVGKLRVELEAEEKTSAARLEALDRTHKMFQREQCRAEAAEKRIAELEKSEEQL
                     INERDHAESALADMYFAATGNWPEWSNCFSFSDAVDAVVDRIADLEAKQPSPVVPEEI
                     STTHTMLPAMNAQLMAFGYVKGWNACRAAMLQGSQPVSQTYKLPVNTPCQDAPAHIWL
                     QTAGVWPEDGELSELTWCSHNQHHDDTLYVRADLVNGNSPVTPDGWISCSERMPNDKQ
                     YVWCWGKSYGWTECDTFEGYYDWSRNKWWAITDDGEEPASKVTHWMTLPEPPQEVK"
     gene            3466..3681
                     /locus_tag="ACHKW5_005015"
     CDS             3466..3681
                     /locus_tag="ACHKW5_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514210.1"
                     /translation="MNNLMTTKQVADFCGVSVSTVLRWNSVNRRTGQKYRPDFPDPDI
                     KSCPNKWASRKIYKFAGVIEECEAAQI"
     gene            3912..>4857
                     /locus_tag="ACHKW5_005016"
     CDS             3912..>4857
                     /locus_tag="ACHKW5_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HFO7514211.1"
                     /translation="MIDNEVKIFAKEKEKMLCNKLSFYREHAQSSEDTRKRNEFERDY
                     ARILYSSSFRRLQGKMQLFEVDPQKFNRNRLTHSLEVAQIARSIASELELEHPVVAEL
                     AALAHDIGNPPFGHSGEKKLNDIANDFGGYEGNAQALRILRSLEIKHPNCPGLNLTHR
                     AILSVVKYPYKKENGRKKYLYNDDYKYYTELVNRYELDLQTGEKTIDAQIMDLSDEIA
                     YAAHDLEDALSRSMVSIEDIFYEFGISKYKDSIDLLNKIIEKSKYTASRASRLNSSEE
                     FAIIFRKELTSNIVNALVNDITVIQSENGFKQLGFSTTK"
BASE COUNT         1458 a         1029 c         1154 g         1216 t
ORIGIN      
        1 ctttccactg ccagcatcga tgactttacc cgttattcta aagatcttgc agatgaaggc
       61 acccgctgct ttatcgatgc cgataatatg cgtgccgtca gtgtgcttaa cctgggtact
      121 attgatgaac caggtcacgc agataacacc gccactctca agctaaaaaa gacagcaccg
      181 ttttctgctc tgttgtctgt taatggcgag cgtaactccc agaagtcact ggcagaatgg
      241 attgaagact gggccgacta ccttgtgggc tttgatgcta atggtgacgc tattcaggca
      301 acaaaagcgg ctgcggctgt ccgtaaaatc acgattgaag caaaccagac cgctgatttt
      361 gaagataatg acttcagcgg caaacgctcc ctgatggagt ctgtcgaagc gaagaccaaa
      421 gatattatgc cagtggcatt tgaatttaaa tgcgttccgt ttgaaggtct gaaagaacgt
      481 ccatttaaat tacgcctcag cattatcact ggcgatcgtc ctgtactggt tctgcgcatt
      541 attcagctgg aagcagtgca ggaagaaatg gctaacgaat ttcgtgatct gcttgttgag
      601 aaattcaaag acagcaaagt agaaaccttt attggtactt tcaccgcctg atttcattac
      661 tgcaaatgcc cctgcggggg catttatgga aacgtaatta actcaataat cgccggatgg
      721 tgagggcttc cttttaccag aattcagcgc ggtgcagcgc atatacgtgg agaacaaaat
      781 gtcatttatt aaaacttttt ccggtaagca tttttattat gacaggataa ataaagacga
      841 catcgatatt aacgatatcg cggtttccct ttcaaatatc tgtcgctttg ccggtcatct
      901 ttcgcacttc tacagcgtcg cccaacatgc ggttctttgc agccagctgg taccgcagga
      961 atttgctttt gaagcgttaa tgcatgatgc aacagaagcg tattgccagg acatccccgc
     1021 tccactgaaa cgccttcttc ctgactataa acagatggaa gaaaaaatag acgccgtaat
     1081 ccgtgagaaa tacgggttac ccccggttat gagtacgccc gtgaaatatg ccgatctcat
     1141 catgctggca accgaacgcc gcgatctcgg gcttgatgat ggctctttct ggcctgtact
     1201 ggaaggcatc ccggcaacag agatgttcaa cgtgattcca ctggcaccgg gccatgccta
     1261 cgggatgttt atggaacgtt ttaacgagtt atcggagtta cgcaaatgcg catgaatgtt
     1321 ttcgaaatgg aagggtttct tcgcgggaaa tgtgtaccgc gagatctgaa agtgaatgaa
     1381 acaaatgctg agtatctggt gcgtaaattc gatgaagtac gtgctgaggc tcgcaacgag
     1441 ggtattaact ataccgcaag ccgccttgct gctgcgttca atcacggatt tatcaataag
     1501 tctttacgtg aagttttcga cgttacacgc atgattctgt cagcgaaaga tgagttggct
     1561 aatgaaccgc acccgattga tggtctgtcc ggtgaatatg cggagaaatc ccttgaagaa
     1621 tgggcggaac agattcgcaa aggaggcaac cagtgaataa ccgcttttac atgatgtgct
     1681 tgcgtgaaac tgtgggtaat aacgcttcat tccattgcca taacggcaat ggttacagtt
     1741 ctaatatcga tcgcgctcat gtttacacgc tggaagaagc ccaaaaagcc tggaattgtg
     1801 gtcgagatat cgatcagcct gtttgcgctg atagcgtgga tgcaatggct gtgtggcacg
     1861 ttgattgcca gtacatccct acagaaaacc tgattgagcc agattgcact gcgtatgtgg
     1921 cctacaaaaa aggtagctgg aacggcaacg atgtttactg gcttcaacac ggtggattgc
     1981 caacagatga cttcagtaaa gcgaccatct ttagcgtcgc caacaaaaac gaaccaggaa
     2041 tagtttggtt gccattttcc attgctgatt cagcaaagcg ccggacgttc aatatcaata
     2101 actttaaccg cagaacaatg gttcagggcg caggtttggt catgcctgac tggttgaaaa
     2161 agcagaacag aagaaagaag tcgcgaagcg ggaaggtgcg ttggaattgt ccgcattgcg
     2221 gaaaaatcac ctggcagtac aacccatatg attttgaagg ctgtagtgat tacaactgtg
     2281 aaggatggcg agaatgacaa ttgactatca ggcactgcgt gaaaaggcag agaaagcaac
     2341 taaaggaagc tacatcgtag ggcatacatc tgttaaccaa cacggcaatt taacaggagt
     2401 ttttgtttgt caaaaatgga aaggagaacc cggtggcgtg attgcggaat gtcatgttaa
     2461 ctgcctgatt gaaacagatg ttcaggctta tgcaaacgct gaatttattg ctgcttttaa
     2521 tccaaatgtt gcgctggcac tactggatga acgggaaaga aaccagcaat acatcaaatc
     2581 ccgcgaccag gagaacgagg atattgcgct tacggttggg aagctgcgcg ttgagcttga
     2641 agcagaagag aaaacatcag cagccagact tgaggcgctc gaccgcaccc acaaaatgtt
     2701 ccaacgggaa caatgcaggg cagaagccgc agagaagcgt attgctgaac tggagaaaag
     2761 cgaagagcaa ctcattaacg agcgtgacca tgctgaatct gctttagcag atatgtactt
     2821 tgcagcaacc gggaactggc cggagtggag taactgtttc agtttttcag atgctgtcga
     2881 tgccgtagtt gacagaattg ctgatttaga agccaaacag ccatcgccag tagtgccgga
     2941 ggaaatcagt acgacccaca caatgctgcc cgccatgaat gctcagttga tggcgttcgg
     3001 ttatgtgaag gggtggaatg cctgccgcgc ggccatgctt cagggtagcc aacctgtaag
     3061 ccaaacttac aagttgccag ttaatacacc ttgccaagat gcgccagccc atatctggct
     3121 gcaaacagct ggagtatggc cagaagatgg cgagttaagc gaattaacgt ggtgcagcca
     3181 caatcagcac catgatgaca cgctatatgt tcgagctgac cttgtgaatg gcaactctcc
     3241 ggtaactccg gatggttgga taagctgtag tgagcgaatg ccgaacgata aacagtatgt
     3301 ttggtgttgg gggaagtctt acggctggac tgagtgcgat accttcgaag ggtattacga
     3361 ttggtcgaga aacaaatggt gggcaattac tgacgatggg gaagaaccgg catcgaaagt
     3421 aacccactgg atgacgctac cggagccacc gcaggaggtg aagtaatgaa caacttaatg
     3481 acaactaaac aagtcgccga cttctgtggc gtttcagtat cgaccgttct tcgctggaac
     3541 agtgtaaaca ggagaactgg ccagaaatac aggccagact ttccagatcc tgatattaaa
     3601 tcctgcccaa ataaatgggc atcacgtaag atttacaaat ttgccggagt tattgaggaa
     3661 tgtgaagcag ctcagatatg agatgacaca tcctatagca catagccaaa catagcaatc
     3721 agactgtccg ctttgtgttg aagcggacat tgccgcagcg acttgtctta gccataaata
     3781 accaaaacga tgcgctgttc atataaagtt cttttaaact tcaaatcagt aatttttgtc
     3841 taaatctttt caaactcata aaaaccagta agataagaaa tcacctttac ctgtttttat
     3901 gagaggcata aatgattgat aatgaagtta aaatttttgc aaaagaaaaa gaaaaaatgc
     3961 tgtgcaacaa actttctttt tacagggagc atgctcaatc gagtgaagat acaagaaaaa
     4021 gaaacgaatt tgaacgagac tatgcgcgta ttctttattc ctcttcgttc cgccggttac
     4081 aggggaaaat gcagctcttt gaagtcgatc ctcaaaaatt taaccgtaat agactgactc
     4141 atagcctaga agttgcccag atagcccgaa gcatcgcatc cgaactagaa cttgaacatc
     4201 ctgttgtagc agaactggct gcacttgctc acgatattgg caaccctccg tttggtcatt
     4261 ccggtgaaaa aaagctaaac gatattgcta atgatttcgg cggctacgaa ggcaatgcac
     4321 aggcacttag aatactcaga agtctcgaga taaagcatcc aaattgcccc ggtctcaacc
     4381 ttacccaccg tgccattctc tccgtcgtta aatatcctta taaaaaggaa aacggcagaa
     4441 aaaaatatct ttataatgat gactataaat actacacaga attggtaaat agatatgaat
     4501 tagatttgca gactggcgag aagaccattg atgcacagat aatggatctg tcagacgaaa
     4561 ttgcatatgc cgcgcatgac cttgaagatg cactaagccg tagcatggtc agtattgaag
     4621 atatttttta tgagtttggg atatccaagt acaaagactc aattgattta ttaaataaaa
     4681 tcattgaaaa atctaaatat acagcttcga gagcatcaag actcaattca tctgaagagt
     4741 tcgccataat attcagaaag gaattgacat ccaatattgt taatgcgctt gttaatgata
     4801 ttactgttat tcaaagtgaa aatggattca agcaacttgg atttagcact acaaaaa
//
LOCUS       DAZFYC010000218         4842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFYC010000218 DAZFYC010000000
VERSION     DAZFYC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.223"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..416
                     /locus_tag="ACHKW5_005017"
     CDS             18..416
                     /locus_tag="ACHKW5_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7514212.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            428..781
                     /locus_tag="ACHKW5_005018"
     CDS             428..781
                     /locus_tag="ACHKW5_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7514213.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            793..1371
                     /locus_tag="ACHKW5_005019"
     CDS             793..1371
                     /locus_tag="ACHKW5_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514214.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            1368..1763
                     /locus_tag="ACHKW5_005020"
     CDS             1368..1763
                     /locus_tag="ACHKW5_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7514215.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1771..2511
                     /locus_tag="ACHKW5_005021"
     CDS             1771..2511
                     /locus_tag="ACHKW5_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514216.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPSSTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            2527..2949
                     /locus_tag="ACHKW5_005022"
     CDS             2527..2949
                     /locus_tag="ACHKW5_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7514217.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            2931..3365
                     /locus_tag="ACHKW5_005023"
     CDS             2931..3365
                     /locus_tag="ACHKW5_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7514218.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            3358..4842
                     /locus_tag="ACHKW5_005024"
                     /pseudo
     CDS             3358..4842
                     /locus_tag="ACHKW5_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
BASE COUNT         1111 a         1205 c         1566 g          960 t
ORIGIN      
        1 tctctgtgga ggagtccatg acgaaagatg aactgattgc ccgtctccgc tcgctgggtg
       61 aacaactgaa ccgtgatgtc agcctgacgg ggacgaaaga agaactggcg ctccgtgtgg
      121 cagagctgga agaggagctt gatgacacgg atgaaactgc cggtcaggac acccctctca
      181 gccgggaaaa tgtgctgacc ggacatgaaa atgaggtggg atcagcgcag ccggataccg
      241 tgattctgga tacgtctgaa ctggtcacgg tcgtggcact ggtgaagctg catactgatg
      301 cacttcacgc cacgcgggat gaacctgtgg catttgtgct gccgggaacg gcgtttcgtg
      361 tctctgccgg tgtggcagcc gaaatgacag agcgcggcct ggccagaatg caataacggg
      421 aggcgctgtg gctgatttcg ataacctgtt cgatgctgcc attgcccgcg ccgatgaaac
      481 gatacgcggg tacatgggaa cgtcagccac cattacatcc ggtgagcagt caggtgcggt
      541 gatacgtggt gtttttgatg accctgaaaa tatcagctat gccggacagg gcgtgcgcgt
      601 tgaaggctcc agcccgtccc tgtttgtccg gactgatgag gtgcggcagc tgcggcgtgg
      661 agacacgctg accatcggtg aggaaaattt ctgggtagat cgggtttcgc cggatgatgg
      721 cggaagttgt catctctggc ttggacgggg cgtaccgcct gccgttaacc gtcgccgctg
      781 aaagggggat gtatggccat aaaaggtctt gagcaggccg ttgaaaacct cagccgtatc
      841 agcaaaacgg cggtgcctgg tgccgccgca atggccatta accgcgttgc ttcatccgcg
      901 atatcgcagt cggcgtcaca ggttgcccgt gagacaaagg tacgccggaa actggtaaag
      961 gaaagggcca ggctgaaaag ggccacggtc aaaaatccgc aggccagaat caaagttaac
     1021 cggggggatt tgcccgtaat caagctgggt aatgcgcggg ttgtcctttc gcgccgcagg
     1081 cgtcgtaaaa aggggcagcg ttcatccctg aaaggtggcg gcagcgtgct tgtggtgggt
     1141 aaccgtcgta ttcccggcgc gtttattcag caactgaaaa atggccggtg gcatgtcatg
     1201 cagcgtgtgg ccgggaaaaa ccgttacccc attgatgtgg tgaaaatccc gatggcggtg
     1261 ccgctgacca cggcgtttaa acaaaatatt gagcggatac ggcgtgaacg tcttccgaaa
     1321 gagctgggct atgcgctgca gcatcaactg aggatggtaa taaagcgatg aaacatactg
     1381 aactccgtgc agccgtactg gatgcactgg agaagcatga caccggggcg acgttttttg
     1441 atggtcgccc cgctgttttt gatgaggcgg attttccggc agttgccgtt tatctcaccg
     1501 gcgctgaata cacgggcgaa gagctggaca gcgatacctg gcaggcggag ctgcatatcg
     1561 aagttttcct gcctgctcag gtgccggatt cagagctgga tgcgtggatg gagtcccgga
     1621 tttatccggt gatgagcgat atcccggcac tgtcagattt gatcaccagt atggtggcca
     1681 gtggctatga ctaccggcgc gacgatgatg cgggcctgtg gagttcagcc gatctgactt
     1741 atgtcattac ctatgaaatg tgaggacgct atgcctgtac caaatcctac aatgccggtg
     1801 aaaggtgccg ggaccaccct gtgggtttat aaggggagcg gtgaccctta tgcgaatccg
     1861 ctttcagacg ttgactggtc gcgtctggca aaagttaaag acctgacgcc cggcgaactg
     1921 accgctgagt cctatgacga cagctatctc gatgatgaag atgcagactg gactgcgacc
     1981 gggcaggggc agaaatctgc cggagatacc agcttcacgc tggcgtggat gcccggagag
     2041 caggggcagc aggcgctgct ggcgtggttt aatgaaggcg atacccgtgc ctataaaatc
     2101 cgcttcccga acggcacggt cgatgtgttc cgtggctggg tcagcagtat cggtaaggcg
     2161 gtgacggcga aggaagtgat cacccgcacg gtgaaagtca ccaatgtggg acgtccgtcg
     2221 atggcagaag atcgcagcac ggtaacagcg gcaaccggca tgaccgtgac gccttccagc
     2281 acctcggtgg tgaaagggca gagcaccacg ctgaccgtgg ccttccagcc ggagggcgta
     2341 accgacaaga gctttcgtgc ggtgtctgcg gataaaacaa aagccaccgt gtcggtcagt
     2401 ggtatgacca tcaccgtgaa cggcgttgct gcaggcaagg tcaacattcc ggttgtatcc
     2461 ggtaatggtg agtttgctgc ggttgcagaa attaccgtca ccgccagtta atccggagag
     2521 tcagcgatgt tcctgaaaac cgaatcattt gaacataacg gtgtgaccgt cacgctttct
     2581 gaactgtcag ccctgcagcg cattgagcat ctcgccctga tgaaacggca ggcagaacag
     2641 gcggagtcag acagcaaccg gaagtttact gtggaagacg ccatcagaac cggcgcgttt
     2701 ctggtggcga tgtccctgtg gcataaccat ccgcagaaga cgcagatgcc gtccatgaat
     2761 gaagccgtta aacagattga gcaggaagtg cttaccacct ggcccacgga ggcaatttct
     2821 catgctgaaa acgtggtgta ccggctgtct ggtatgtatg agtttgtggt gaataatgcc
     2881 cctgaacaga cagaggacgc cgggcccgca gagcctgttt ctgcgggaaa gtgttcgacg
     2941 gtgagctgag ttttgccctg aaactggcgc gtgagatggg gcgacccgac tggcgtgcca
     3001 tgcttgccgg gatgtcatcc acggagtatg ccgactggca ccgcttttac agtacccatt
     3061 attttcatga tgttctgctg gatatgcact tttccgggct gacgtacacc gtgctcagcc
     3121 tgtttttcag cgatccggat atgcatccgc tggatttcag tctgctgaac cggcgcgagg
     3181 ctgacgaaga gcctgaagat gatgtgctga tgcagaaagc ggcagggctt gccggaggtg
     3241 tccgctttgg cccggacggg aatgaagtta tccccgcttc cccggatgtg gcggacatga
     3301 cggaggatga cgtaatgctg atgacagtat cagaagggat cgcaggagga gtccggtatg
     3361 gctgaaccgg taggcgatct ggtcgttgat ttgagtctgg atgcggccag atttgacgag
     3421 cagatggcca gagtcaggcg tcatttttcc ggtacggaaa gtgatgcgaa aaaaacagcg
     3481 gcagtcgttg aacagtcgct gagccgacag gcgctggctg cacagaaagc ggggatttcc
     3541 gtcgggcagt ataaagccgc catgcgtatg ctgcctgcac agttcaccga cgtggccacg
     3601 cagcttgcag gcgggcaaag tccgtggctg atcctgctgc aacagggggg ccacctcgct
     3661 ggcggtggcg accggtgcgc tggcgtatgc ctggtatcag ggcaactcaa ccctgtccga
     3721 tttcaacaaa acgctggtcc tttccggcaa tcaggcggga ctgacggcag atcgtatgct
     3781 ggtcctgtcc agagccgggc aggcggcagg gctgacgttt aaccagacca gcgagtcact
     3841 cagcgcactg gttaaggcgg gggtaagcgg tgaggctcag attgcgtcca tcagccagag
     3901 tgtggcgcgt ttctcctctg catccggcgt ggaggtggac aaggtcgctg aagccttcgg
     3961 gaagctgacc acagacccga cgtcggggct gacggcgatg gcacgccagt tccataacgt
     4021 gacgacggag cagattgcgt atgttgctca gttgcagcgt tccggcgatg aagccggggc
     4081 attgcaggcg gcgaacgagg ccgcaacgaa agggtttgat gaccagaccc gccgcctgaa
     4141 agagaacatg ggcacgctgg agacctgggc agacaggact gcgcgggcat tcaaatccat
     4201 gtgggatgcg gtgctggata tcggtcgtcc tgataccgcg caggagatgc tgattaaggc
     4261 agaggctgcg tttaagaaag cagacgacat ctggaatctg cgcaaggatg attattttgt
     4321 taacgatgaa gcgcgggcgc gttactggga tgatcgtgaa aaggcccgtc ttgcgcttga
     4381 agccgcccga aagaaggctg agcagcagac tcaacaggac aaaaatgcgc agcagcagag
     4441 cgataccgaa gcgtcacggc tgaaatatac cgaagaggcg cagaaggctt acgaacggct
     4501 gcagacgccg ctggagaaat ataccgcccg tcaggaagaa ctgaacaagg cactgaaaga
     4561 cgggaaaatc ctgcaggcgg attacaacac gctgatggcg gcggcgaaaa aggattatga
     4621 agcgacgctg aaaaagccga aacagtccgg cgtgaaggtg tctgcgggcg atcgtcagga
     4681 agacagtgct catgctgccc tgctgacgct tcaggcagaa ctccggacgc tggagaagca
     4741 tgccggagca aatgagaaaa tcagccagca gcgccgggat ttgtggaagg cggagagtca
     4801 gttcgcggta ctggaggagg cggcgcaacg tcgccagctg tc
//
LOCUS       DAZFYC010000219         4818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFYC010000219 DAZFYC010000000
VERSION     DAZFYC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.224"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..2354
                     /locus_tag="ACHKW5_005025"
     CDS             93..2354
                     /locus_tag="ACHKW5_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO7514219.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2496..3779)
                     /locus_tag="ACHKW5_005026"
     CDS             complement(2496..3779)
                     /locus_tag="ACHKW5_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO7514220.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3914..>4818)
                     /locus_tag="ACHKW5_005027"
     CDS             complement(3914..>4818)
                     /locus_tag="ACHKW5_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514221.1"
                     /translation="SGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINY
                     MSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEG
                     HITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDL
                     CEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIAS
                     TRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLG
                     HKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1168 a         1176 c         1231 g         1243 t
ORIGIN      
        1 cttgtcgcct tcctgcaact caaattattt agagtatatc catttattat ctttctgcgc
       61 acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt
      121 ttctcgccag taataacgaa ataattgctg aagaacattt caccggcgaa attaaaaaag
      181 aagaagccaa aaaaggcact attgcctggt ctattctctc ttcgcataat acgtccggaa
      241 atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg
      301 gtattgtaca gaccgctaaa gcgtccggta tggaacgttt cccgctgccg tatgtgctga
      361 ccaactgcca taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt
      421 ttggtttatc ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca
      481 tccatcaata tatgcgtgag atgatggcag gcggcggcaa aatgatcctc gggtcagaca
      541 gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggcggt ggtgagttgg
      601 taaaacagct gcttaatgac acctgggata tcgactatcc gggagttgtt gcggtgcatc
      661 tgaccggaaa accagcgccg tatgtggggc cgcaggatgt ggcgctggct atcattggcg
      721 cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta
      781 gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt
      841 taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg
      901 gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg
      961 ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg
     1021 aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg
     1081 aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc
     1141 tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg
     1201 cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcagttt
     1261 acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga
     1321 ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata
     1381 cgccaatcaa caacggatta agtattcgcc acaccacgcg taacttcccg aaccgcgaag
     1441 gctctaagcc agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg
     1501 ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca
     1561 acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg
     1621 tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat
     1681 tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtga
     1741 ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg
     1801 gtctggcgga gtttaccctg tcacgccgcg atcccggtta tgttggcaga agtaaagcga
     1861 ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg
     1921 cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca
     1981 gcatggtata tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc
     2041 agcgtgtgat tggcggtctg gcgaatattg ccgaagagta cgcgactaaa cgctaccgtt
     2101 ctaacgtcat caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag
     2161 tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt
     2221 ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa
     2281 gtctgactgc tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa
     2341 accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat
     2401 gcgatgcttg cgcatcttat ccgacctacg aatcgcatcg aatctgtagg ccggataagg
     2461 cgtttacgcc gcatccggca aatagttaat agctcttact tcttcgcctc tgcaaccact
     2521 ttactaccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga
     2581 ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat tacccgcaaa cggacgatta
     2641 gagatcaccg catcggccca cggtttagcc gtgttaaaac cttcgttgat ggcgctatca
     2701 cggatcacca cctgaccgtt ggtattggca tcaacatcca gcgagcggcc cagttgcgcc
     2761 acaccatcac cgaaagcatt gaaacggctg tttacggcga ggaaaccgta gtaaatgttg
     2821 gacagcgtag ccggtgcaaa cacatacgct tcttgctgag tacgtgagtt caccacgcgg
     2881 aattcggtgt tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca
     2941 atgtagctgt tggtcaccag cgtacgcggc tgacggttgg tttccagacg gttctgcaca
     3001 ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt gttaatctgt
     3061 acctggtcac catcagtacg cagtgccacc gccggatggt tacctgcatc tacgctatcg
     3121 cccagcgtgt tttcgatggt cagattttgc agttgcaggc cattgttttg tgaccagaag
     3181 accgcagagc agagaacacc gatactgtcg ctgcgtttgc tctggcagct atcgtacata
     3241 taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg
     3301 gcagggctca tgcccccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct
     3361 gtaccgtaca gagtaattcc acccggagca gcagggacat acaccgttcc ctgatactca
     3421 ccaggcatca cggcaatata ctggcgcttg ttagtgcgct tgataattgc cgcatctacc
     3481 gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca
     3541 ggttgcgcag gcagggtaat cggggaagga ttccacgctg cagcacctgg tgtcagggat
     3601 gcaaaatagt gttgagcatc gaaattctgc gcttcttttg ccgacagaat cgggcgagaa
     3661 gaggtaccag gcgcggtttg atcagaagga cgttgatcgg gtggtgttga gctacaggcg
     3721 gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca
     3781 ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa
     3841 gttggcatta taaaaaaaca ttgcttatca atttgttgca acgaacaggt cactatcagt
     3901 caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat
     3961 actgtgatgc catggtgtcc gacttatgtc cgagaagatg ttgagcaaac ttatcgctta
     4021 tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggcggatccc
     4081 cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg
     4141 atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctctttgc
     4201 atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgtaggga
     4261 tggcaatttt tacgcctgtt ttgctttgtt cgacataaag atatccatct acgatatcag
     4321 accacttcat ttcgcataaa tcaccaactc gctgcccggt aacaacagcc agttccattg
     4381 caagtctgag ccaacatggt gatgattctg ctgcttgata aattttcagg tattcgtcag
     4441 ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg agtggcagcg accgggtttg
     4501 ttgttatatg gccttcagct attgcctctc ggaatgcatc gctcagtgtt gatctgatta
     4561 acttggctga cgccgccttg ccctcgtcta tgtatccatt gagcattgcc gcaatttctt
     4621 ttgtggtgat gtcttcaagt ggagcatcag gcagccccct ccttattgct ttaattttgc
     4681 tcatgtaatt tatgagtgtc ttctgcttga ttcctctgct ggcgaggatt ttttcgtagc
     4741 gatcaagcca tgaatgtaac gtaacagaat tatcactgtt gattctcgct gtcagaggct
     4801 tgtgtttgtg tcctgaaa
//
LOCUS       DAZFYC010000220         4707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFYC010000220 DAZFYC010000000
VERSION     DAZFYC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.225"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..152)
                     /locus_tag="ACHKW5_005028"
     CDS             complement(<1..152)
                     /locus_tag="ACHKW5_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO7514222.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNL"
     gene            complement(218..580)
                     /gene="yfdP"
                     /locus_tag="ACHKW5_005029"
     CDS             complement(218..580)
                     /gene="yfdP"
                     /locus_tag="ACHKW5_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO7514223.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(1038..1691)
                     /locus_tag="ACHKW5_005030"
     CDS             complement(1038..1691)
                     /locus_tag="ACHKW5_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7514224.1"
                     /translation="MMNMSDRIRQRRKELNLTQQALADLTGVNRVTVTGWEKDDYQPN
                     GANLQALANALKCDPLWLVSGKGSPEPKINLKPEIFAVKKVPLISWVQAGSWTMTEPG
                     VRKEDAEEWVYTTALVSEMAFALRVRGDSMTNPLGSPSIPEGSIVIVEPDIIDTECIN
                     GKIVVAHINGGQEATLKKFVEDWPNRYLVPLNPNYKTIECGENCRIVGLVKQVIMDF"
     gene            1787..1984
                     /locus_tag="ACHKW5_005031"
     CDS             1787..1984
                     /locus_tag="ACHKW5_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7514225.1"
                     /translation="MRKSDVINYFGGVCKTAEALGIKHPSVSEWPEIIPEGRAYQLEK
                     ITNGKLKVDVSLYQKTNSAAA"
     gene            2012..2596
                     /locus_tag="ACHKW5_005032"
     CDS             2012..2596
                     /locus_tag="ACHKW5_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfL family putative regulatory protein"
                     /protein_id="HFO7514226.1"
                     /translation="MGKEPEWKVDKQPAWLVAAIRRTIADLPHGYEEAAEILGLYKSD
                     DITPAKDQLHNRLRSGGDQIFPLEWAMVLQDASGTRHVTDAIARRSYGVFVPLVVIDD
                     IDNGDINQRLMESIEWIGKHSQYLRKATADGVIDQAEREQIEENSYQVMAKWQEHLTL
                     LFRVFCAPEKSNARECAAPGVVASIASGCGETNA"
     gene            2742..2921
                     /locus_tag="ACHKW5_005033"
     CDS             2742..2921
                     /locus_tag="ACHKW5_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7514227.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAY"
     gene            2851..3741
                     /locus_tag="ACHKW5_005034"
     CDS             2851..3741
                     /locus_tag="ACHKW5_005034"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7514228.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSGIPGRYTVQAPYKAGAGGR
                     SPELSTAIYDAPASFLSSALTHARIMVGWAGEPKGSPVSVDAGTANPVQSATSEFRSS
                     GGGSFPQSTEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTLE
                     CSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFNQ
                     MEKQLSKPSVPSDVAHNANVLYSYISSIHQVWLQQLYPMLAKAESPLAVSLYDRINDA
                     AFLARLIHSSLNSSEVRGRK"
     gene            3738..3962
                     /locus_tag="ACHKW5_005035"
     CDS             3738..3962
                     /locus_tag="ACHKW5_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514229.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRISMPLIAFCSGKNMKHLGGAA"
     gene            3959..>4707
                     /locus_tag="ACHKW5_005036"
     CDS             3959..>4707
                     /locus_tag="ACHKW5_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514230.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVKPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSVAGR"
BASE COUNT         1208 a         1073 c         1166 g         1260 t
ORIGIN      
        1 tttaaattaa atttttccag atcatgaatg cggaaattct caggcaatgc cacagcatcg
       61 gcaccaatct tactgataat ttcattaaca ccctgagcag aaataagggc atggatttga
      121 ttaattgcgg ttgcgtctaa gttctgagac ataataagtc ctcactatat aaagatattc
      181 agtgatgaga taaataatca gttaattaag aacgatatta atgacctgct gcgcgtagtt
      241 ttccgtcagg ttcaccggca agagtcagta attgtccctg gtcttcctgc agaatagtca
      301 ggcgaccacc gcgattgaca tacatcggcg tttcggtggt gtcttcttcg gaaattttcc
      361 cgcggttagt cgggcgaaca tatgagagtt tgtgtttgat tttcacacgg ttctcatcaa
      421 acggttcgat ttccaggttg agcgagacct tacctttggt tttcgtgttc atcacaccgg
      481 aagcgacttc actgagaaca gcgccgattt tggtttcaaa tacgccgccg tccaactccc
      541 cgataaatgc ctgcacatca gtactgcgtt cgctagccat tttgctgctc ctcatcatat
      601 cgaccctgca aggtcggttg gtttctccac aaaacagaga agaacacctg cggtggcagc
      661 cgcccgggtg gattgggtta tgagcccgtc gtccggtgat gctcttctct gttttgtaaa
      721 aagagcggta ccagccggaa gcaagtgtac aaactggtac cgccaaagca gtggctgttg
      781 tggtgatcgg tgctgatctc cggcttgcgg ttatttcaga ctctcacggg catttaattg
      841 ccccgccgaa cagctctttt ccgtaatagc tgcaatgtct ttcgcgcatc agcctgcgca
      901 ttcaccacaa ctctaaaaac aaatgtagga tatccaacat gtgagtgtca agagtttatg
      961 ttggttatcc tacataaaaa gataggctca taaaaaaacc ggggataccc cggttttgcg
     1021 atagtgagga agatgtgtca aaaatccatt attacttgtt tgacaagacc aactattctg
     1081 cagttctcac cgcattcaat agttttatag ttaggattta gtgggacgag atacctgttc
     1141 ggccagtcct caacaaattt tttgagtgtc gcttcttgcc caccattgat atgggcaaca
     1201 acgatttttc cgttaataca ctctgtatca ataatatctg gctctacgat aacgatagaa
     1261 ccttctggta tcgatggtga gccgagggga ttggtcattg aatcaccacg gacccgtagt
     1321 gcaaatgcca tttctgatac aagggcggta gtataaaccc actcttcagc atcttctttc
     1381 ctgacaccag gctccgtcat tgtccatgaa cccgcctgaa cccacgagat gagggggact
     1441 tttttaactg cgaatatttc aggttttaga tttatctttg gttcaggcga gccttttccg
     1501 ctaacaagcc acagaggatc gcatttaagt gcgttggcta gggcttgaag gttggctcca
     1561 tttggttggt agtcgtcctt ttcccatcca gtaaccgtga cacggttcac accagtcaaa
     1621 tcagccagtg cttgttgtgt caggttcagt tcttttcgcc tttggcgaat acgatcactc
     1681 atgttcatca tgtaggcaat cctaccacat gactatgtag gattcttgac attggtatgt
     1741 tggatatcct acatttctgc ttaacgtaat ttaacgggag acagaaatgc ggaaatccga
     1801 cgtgattaat tatttcggcg gagtttgtaa aaccgccgaa gccctaggta ttaagcatcc
     1861 gtctgtttca gagtggcctg agattattcc tgaaggccga gcgtaccagt tagaaaaaat
     1921 tactaacggg aaactgaaag ttgacgtgtc tttatatcaa aagactaaca gtgctgcggc
     1981 ataaaaacac cacagaaatg aggaattaac cgtgggtaaa gaacctgaat ggaaagttga
     2041 taaacaacca gcatggctgg tggcagcaat acgaagaacg attgctgatt tacctcatgg
     2101 ctatgaggaa gcagcagaaa ttcttggttt gtataaatct gatgatatca ccccagcaaa
     2161 agatcaattg cataacagac tgcgtagcgg tggggatcaa atttttccac ttgagtgggc
     2221 catggtttta caggatgcca gtggtaccag gcatgtaaca gatgcaatag cccgtcgtag
     2281 ttatggggtg tttgtgccgc tggtggtcat tgatgacatt gacaatggtg acattaatca
     2341 gcggctgatg gagtcaatag aatggattgg caagcattcc cagtacttac gcaaggcaac
     2401 tgctgatgga gttattgacc aggctgagcg tgagcaaatc gaagagaaca gctaccaagt
     2461 aatggcgaag tggcaggagc atttaacact gttatttcgt gttttttgtg caccggaaaa
     2521 gagtaacgcc cgcgagtgtg cagctccggg cgtcgtggcg tcgattgctt ctggttgtgg
     2581 agaaactaac gcatgaacag tttaacggca aataaccgtt tgtcgcaaca gctggtggtc
     2641 agcgtctctg aacacctgtt gttacggcat gaatgcagat taccaaatca cctggctgta
     2701 agtaaccaca gagaacttta cctgactgtg gggggcgagt tgtgcaggaa cttaaccgct
     2761 ggtttcgtga cggaagagga ctttatgttc atgttattcg ttgggagcca gaaacacagc
     2821 gcgttatcta tcttcgcaaa gactacccac atgagtgctt tagtcctttg tggaaattca
     2881 ggcgtgattt tgttgagtgt gaaggaccac cagcatattg attctggaat tccgggacgt
     2941 tacactgttc aggcacctta taaagcgggt gccgggggtc gcagcccgga attgtcaacg
     3001 gcgatatacg acgcgccagc gtctttttta tcgtccgcgc tcacgcacgc cagaattatg
     3061 gtgggctggg caggggagcc gaaaggctcg ccggtctccg ttgacgccgg tactgcgaac
     3121 cctgttcagt ctgccaccag tgagtttcgc agctccggtg gtggaagttt tccacagtca
     3181 acggaggctg ccatcatggc tacgatccca accctcactc aacctgaaat tgccatcgtt
     3241 gatggtcagg ctgttacttc atccctggct gttgccaact tcttctccaa acgtcatgac
     3301 gatgtactga aaaagatccg cacgcttgaa tgttccgcat cattcactgc ccgcaatttt
     3361 tcggtgagtg attacaccga ttgcacaggc cgcaaactac cttgctatca aataacccgc
     3421 gacggctttg cgtttcttgc tatgggtttc actggtaaac gtgctgccca gttcaaagag
     3481 gcatacatca atgcctttaa ccagatggag aaacagcttt caaagccctc tgtaccgagc
     3541 gacgttgcac ataacgccaa cgttctctat tcctacattt catcaattca tcaggtctgg
     3601 ctgcagcagc tttatcctat gttggcaaaa gctgaatcac cgctggctgt aagtctgtat
     3661 gaccgaatta acgatgcggc atttcttgcc cgtcttattc attcgtcgct gaactcttca
     3721 gaggtaaggg ggcgtaaatg atccggaata ttttcaaacg gtttaccaat cagactttcc
     3781 gttgtcctcg ccccggtcag tggtacacca cacctgcagg gcatgttcta cgtgttagcc
     3841 tggttgaccg tgaatgtcag aaggtgattt gtgaaccgct gggccgtaat taccgcatca
     3901 gtatgccgct tatagccttt tgctccggaa aaaacatgaa gcatctcgga ggtgctgcat
     3961 gagtatggag ctgatggtta aagcgatgaa aattcgagtg ggaaatccat tgcgaaaact
     4021 ggttctgatt aagctggctg ataatgccag cgatcagggc gagtgctggc ccagctacca
     4081 gcatattgct gaccagtgcg agattagcaa acgttctgtg atgaatcata ttgcggccct
     4141 ttgtgagtcc gggctggtaa aaaaagtcac ccggaaaggt gaaaaaggta actcaagtaa
     4201 tatctatctc cttcatctgg atggtgcagg agattcacta gggggtagtg caaataattc
     4261 actatctggt gcagcaaatt caccaggtag tgcaggagtt gcaccagggg gtagtgcagg
     4321 agattcaccc agaaccagtc actcttttga accagtcaaa gaaccagtca atgaaccaat
     4381 agctgttggt gcatctgctg atgagtctgt gcgagttcgt tcaaaccgac cggaatactc
     4441 tccggagttt gagcaggcat ggctggcata ccccaaacgt gctggtggca attcaaaatc
     4501 tgcagccttc aaagcctgga aagcccgttt gaatgagggg gtaaaacccg aaaccatgct
     4561 ggaaggtgtg aaacgctacg cgggctgggt atctgcgatg ggtaacagcg gcacacaatt
     4621 tgtgaaacag gctgtcacgt tctttggtcc ggatcgtcat ttcgaagaat cctgggaagt
     4681 tcctgcggta tctgtagccg gacgtga
//
LOCUS       DAZFYC010000221         4499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFYC010000221 DAZFYC010000000
VERSION     DAZFYC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.226"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..680)
                     /locus_tag="ACHKW5_005037"
     CDS             complement(<1..680)
                     /locus_tag="ACHKW5_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7514231.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGG"
     gene            complement(687..1649)
                     /locus_tag="ACHKW5_005038"
     CDS             complement(687..1649)
                     /locus_tag="ACHKW5_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514232.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(1672..2097)
                     /locus_tag="ACHKW5_005039"
     CDS             complement(1672..2097)
                     /locus_tag="ACHKW5_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7514233.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(2081..2404)
                     /locus_tag="ACHKW5_005040"
     CDS             complement(2081..2404)
                     /locus_tag="ACHKW5_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514234.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            2529..3005
                     /gene="racR"
                     /locus_tag="ACHKW5_005041"
                     /gene_synonym="rarC"
     CDS             2529..3005
                     /gene="racR"
                     /locus_tag="ACHKW5_005041"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO7514235.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFILSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            3324..3479
                     /locus_tag="ACHKW5_005042"
     CDS             3324..3479
                     /locus_tag="ACHKW5_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7514236.1"
                     /translation="MDTIDLGNSESLVCGVFPNQYGTFTAMTYTKSKTFKTESGARRW
                     LERNSGG"
     gene            3481..3609
                     /locus_tag="ACHKW5_005043"
     CDS             3481..3609
                     /locus_tag="ACHKW5_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514237.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEAHSFNEFKQVLSSSAKSNG"
     gene            3639..3857
                     /gene="ydfC"
                     /locus_tag="ACHKW5_005044"
     CDS             3639..3857
                     /gene="ydfC"
                     /locus_tag="ACHKW5_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO7514238.1"
                     /translation="MQKREPVIIAPDYTNDELYEWMHQKINAAQDLKWANEVRAKQAE
                     NLSSLEQDITNLEKAAALTIARMITYPR"
     gene            4426..>4499
                     /gene="dicB"
                     /locus_tag="ACHKW5_005045"
                     /gene_synonym="ftsT"
     CDS             4426..>4499
                     /gene="dicB"
                     /locus_tag="ACHKW5_005045"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7514239.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPV"
BASE COUNT         1150 a         1178 c         1032 g         1139 t
ORIGIN      
        1 ccaccattca tggtcatacg gtccatcacc cgatctccga ggagtgtgct catcgctgcg
       61 tgattcaggt tcgtcagcat tccgacacta cgcatcgaag ccgttctgcg gtcgactatc
      121 tggttcagcg tgacctgctc gttgcgcgta tcccgctgca tgccaatttc atcaaggacc
      181 agaaggtcaa ctccacaaag ctcctgtaaa aatttttccc cggactggcc gttgtcgtag
      241 ccgtcatgca acacgctcat gacatcggac acggtgacga taatcacgct tctccccttc
      301 gccatcagcc gattgccaat cgctgctgcc aggtgatttt taccggtacc aggtttaccg
      361 ctgaacacga agtttgtaca tccggtcatc aattcatcgg caatggattt cgcctggctc
      421 agagcatggc gctgaccgtc gttctgcacc cggtagttcc cgaatgagca cttcctgtga
      481 agcggctgga tgcccgcacg gttcaggatt ttttcaaccc gcacctgatg attcaggcgg
      541 ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc attcccgcca ctcctccacc
      601 gtccggtacg gtggaaccgc cccctgtggt gcaagtctgc gaatacgttc aagaacccca
      661 actgccgcaa tgtttttcat gacacgtcac cccctgaatc ccggcggtat ttcagtgtcc
      721 ggttcagaaa tgtgattcac gcaacgctgc gcaggcgaac gccccaggcg gataaccagt
      781 tcatcccatt tttcccggag ttttgccgga ctcatgatgt tttttaccca gaacgaatcc
      841 cgctggagac gcccaaacat ttcacaaatt tgtctgtgag ttctgccatc cagcatccgc
      901 attgtgcgaa cgtcattggc ccatgctgtc cagttgggtt ctttcggtct agtgatctcg
      961 ccatcatcgc tggccgcctg ctcgtaaaga ctcacgattc gtccccagat ccactgtgcg
     1021 cacaccaaat cttcctgact tccccactgg cgttttttcg cactgaacac aaccgcgtca
     1081 gggtgtcggg ttaaaaaatc ctgttcagcc gtctgcgggt ccggttgcga agcgtccgga
     1141 caagaagatc ttttatctga cggatcaggt tttaatactg acggatcggg gtcaatcatc
     1201 gcccccctaa tcggcagttt tttatcaaca gttgatccat caaaatttga cgggtcaacc
     1261 gttgaggggt caatatttga cgggtcaact gttaacgggt cattttttgc cgggctaatt
     1321 tttcttttcg gtttatatga ctcacgcgcc gccgccgcag ctgcttcgag tttttccaca
     1381 ttaagccgat agatattgct tacattacgc ccaccgacct tacgctcttc cttcgtcagc
     1441 cagccctctt tcgccagttc tgcaatagcc gatttcactg tggattcact tcttgcaccg
     1501 atctgacgcc ggatagtttc aatggcaggc catgacacgc cctcgtcatt gctgtagtct
     1561 gcaagacggg ccataaccgc caccctggat aagatcatgc cggtgaaggc gcacccttcc
     1621 cagacaagac catgaagctt gctgctcata aaacccccga acaccgtgct tttagtgcat
     1681 caccacagca ttccctgccg ggccgccgcg attcatctgg tcatacaaaa caaccgctga
     1741 cgcaacaaaa tcatcgacat ccttcaccag ccgatccctc cgttcgacga tctcacggta
     1801 atattcagaa ctgtggctgc gcatacgggc caccagcaaa ggcggcatcg ccttttcgat
     1861 cgccggtaac agagcctgca ttttttcaac agcatcaggg gtgtctttat ccagccaacg
     1921 gaaaattttc tgggtattac gggccagggc ttccggatgg ctgtcgtcgt acagttcagg
     1981 gaacgtcatc cccagttcga aataagtccg ggctatttca gctgcaggaa ctttctcacc
     2041 atcagggtat gcccaggcat tcatcgccat gcggatgtgc tcatgtttga ttttcatgaa
     2101 tcatttgcct cttgatgctt cgggtatgat cgttttcgtc atttggttgc ttcatcgaca
     2161 tattctgcga ataacatgac gagcgtcgta agtatgtcca atcaacatca ggacgaagtt
     2221 cttcacacag aacaccacct tttgttgctc gttcaatcgc aggacatctc tcagcaggta
     2281 actgacgtac acctttgatc cattgattta cgcttggagg agatacacct aaaagcctag
     2341 ccattgctga ttgcccaccg acaacagcac aagctcgttt gaatgaatag ttatcttttt
     2401 tcatcgaatg aactccaaaa aacacgcaac aatattaggc ttagcctaat gaaattgtca
     2461 ataggctatg cctaatacat cgagagtagg gattgcctaa cgcgatgcgc ataggagact
     2521 attaagcaat gcttagtggt aaagacttag gccgagcgat agagcaggcc attaacaaaa
     2581 aaattgcatc aggagccgtc aaatcaaagg cggaaatcgc acgtcatttc aaagtccaac
     2641 caccatcaat ccatgactgg attaagaaag gttcgataag taaagacaaa cttccagaac
     2701 tatggcgttt cttttctgat gtggttggtc cagagcattg ggggcttaac gaatacccca
     2761 taccaacccc atccacttca gatacaaaaa gtgaactttt agacataaac agcctttatc
     2821 aagccgcctc tgatgaaaaa agagcaattg tggctttcat cttatctgga aatgctacgg
     2881 agcctagttg ggttgatcat gacgttcgcg cctacattgc cgcaatggaa atgaaggtag
     2941 ctaactatct gaaaaatcaa gaatcaaaac ggaaaagcca gaacatcacc aagacaggaa
     3001 cttaaactta tatggtccga cgggaaattc ctagttcccg ttagttaact cctactacct
     3061 cttccacaaa ccatcaccta ttaggttgcg cccaaattat taggtatagc ctattgacaa
     3121 gtaattaggc atttcctata gttttcccat accaacccat cccgtcccac acaatacagg
     3181 gcaatacctc gagttaccag gcagtggtca cgggttaagt agccagcccg aggcgtaaga
     3241 acatgacggc agggttcaac tttaataact atgcagcagg tttttgttcc gctaccccgg
     3301 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacagc gaatctctgg
     3361 tatgtggcgt gttccccaac cagtacggta cgttcaccgc aatgacgtat accaaaagca
     3421 aaacgtttaa aaccgaatct ggagcgcgtc gctggctgga aagaaattca ggtgggtgat
     3481 atggatttcg acacaatcat ggaaaaggct tacgaagaat acttcgaagg ccttgccgaa
     3541 ggcgaagaag ctcacagctt caacgaattt aaacaggtgc tttccagttc ggcaaaatct
     3601 aatggctgat aagcgaaaca gcaccgcgag gaatcagtat gcagaaacga gaacccgtca
     3661 tcatcgcgcc agactatacc aatgatgaac tttatgagtg gatgcaccag aaaattaatg
     3721 cagcgcagga tctgaaatgg gctaatgaag tcagggctaa gcaggctgaa aatctgtcct
     3781 ctctggagca ggatatcacc aatctggaaa aagcagcggc attaaccatt gccagaatga
     3841 ttacataccc gcgttaatag ctaaccaacg aagctaaggt tggtaattaa ggagttctcc
     3901 acgggtgagg tggagtgctt gcgccggaca cgggtgagca tccggcactg acagtttact
     3961 gaaaggatat ttccctgaaa agtcagacca taacgcgaaa gcgcacggcg aggtagctgg
     4021 ttcatagata gcctgtcgtt aaattttcgt cgaccgtgcg cttccggttg tggcaatccg
     4081 cgaaatggcg cggcggtaag tatggcgggg ttattccttc ccccgttgag gacaccgggt
     4141 tgtcaggttg accatacgct taagtgacaa ccccgctgca acgccctctg ttatcaattt
     4201 tctggtgacg tttggcggta tcagttttac tccgtgactg ctctgccgcc ctttttaaag
     4261 tgaattttgt gatgcggtga atgcggctaa gcgcacgcgg aacagttaaa accaaaaaca
     4321 gtgttatggt tggattctct gtatccggcg ttaattgtta actggttaac gtcacctgga
     4381 ggcaccaggc actgcatcac aaaattcatt gttgaggacg cgataatgga aacgttatta
     4441 ccaaacgtta atacgtctga aggttgtttt gaaattggtg tcactatcag taaccctgt
//
LOCUS       DAZFYC010000222         4469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFYC010000222 DAZFYC010000000
VERSION     DAZFYC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.227"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..293)
                     /locus_tag="ACHKW5_005046"
     CDS             complement(<1..293)
                     /locus_tag="ACHKW5_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7514240.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPEYYFVVVLAGQSNSMAYGEG"
     gene            544..672
                     /locus_tag="ACHKW5_005047"
                     /pseudo
     CDS             544..672
                     /locus_tag="ACHKW5_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(777..847)
                     /locus_tag="ACHKW5_005048"
     tRNA            complement(777..847)
                     /locus_tag="ACHKW5_005048"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(817..819),aa:Met,seq:cat)
     gene            complement(993..1418)
                     /locus_tag="ACHKW5_005049"
     CDS             complement(993..1418)
                     /locus_tag="ACHKW5_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514241.1"
                     /translation="MSVMKKVLIAAIGFCLVGCAGMKLPEYSQVKASPYYTDCRAFAM
                     DVYKNDGYSKIAKTTILSMDDVKARYIVTGCVVAMGKNTVEEIKADLSAKGSSFGLIS
                     GACSSAACRVDVEQQMNAYILGSYYAANKKFPDKMKAEF"
     gene            complement(1699..2451)
                     /gene="ydfT"
                     /locus_tag="ACHKW5_005050"
     CDS             complement(1699..2451)
                     /gene="ydfT"
                     /locus_tag="ACHKW5_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7514242.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVIAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPALTDYQ
                     WYKGYADIIDKLVTKCWQEEAYAETQLRKVTR"
     gene            complement(2465..3454)
                     /locus_tag="ACHKW5_005051"
     CDS             complement(2465..3454)
                     /locus_tag="ACHKW5_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514243.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNSARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(3462..4277)
                     /locus_tag="ACHKW5_005052"
     CDS             complement(3462..4277)
                     /locus_tag="ACHKW5_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO7514244.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYS
                     RTGINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT         1194 a         1182 c          948 g         1145 t
ORIGIN      
        1 ccttcaccat atgccatgct gtttgactgc cccgccagaa ccacaacaaa gtaatactcc
       61 gggtcgctgg tggcgctgat tactgcacct tctccacctg tcggcttcac cacaacaggt
      121 gtgctcacat caccttctgc gacaatcgcc tgaataagtg ctgcgccatc atccgtatac
      181 gaagaaaacg gcccgccgta tggttgccat ccttcacgaa ttttttgagc aagtgcatca
      241 gcaaggtctg acggcgacgc cgccctgaca acatcataat gtttaaatgt cattattcct
      301 cccggccggg ataatgttgt gagtcagata aggggcgggc tgaagtccgg aagttacagg
      361 acaatgacag aaggaagact acagcccgca atacgaaaaa ggccgcgcag ttgcgcagcc
      421 ttataaaccc tggttaaaat ccacacgata aaaatgacaa tgcaagtatc tcatgctgtt
      481 gcccgaacct actcgggctt tttttgcatg taaaaaggct cctgcgatga ggagcctgga
      541 tatatgccta atctctgtat acagcatgat gccgggtgcc tcccggtgaa ttcagtacca
      601 gcacctgaat ccgcgattat cccatatacc tactcgctga ttgcccctcc gcacaggggg
      661 attcaccatg ccagtttctt ttaacaaact ccccgcaaac cagacaacag tcaaccgcct
      721 gaattgtgag acatttaaaa aaaaggcccg caaaagcgag ccagggaaaa taagtgtggc
      781 gcgttgtact ggattcgaac cagcgacctg gcgattatgc gtcgctcgct ctcaccactg
      841 agctaaaggg ccgggagcag aataataacg gtccgtaatt aattccgaaa taaaaaaccc
      901 gctcagtggc gggttgctat cacagctata tatttactta ttatgccgtt actaacattt
      961 atcttcggca tataatcgaa aacaaggttt gcttaaaact ctgctttcat tttatccggg
     1021 aattttttat ttgcagcata ataactacca agtatataag cgttcatttg ctgctctaca
     1081 tcaacccgac atgcagcact agaacaagca ccactgataa gcccaaaaga actcccttta
     1141 gcagagagat cagctttaat ttcctctaca gtgtttttcc ccatagcaac tacacaccct
     1201 gtaacaatat atctagcctt cacatcatcc atgctaagga tagtggtttt cgcaattttg
     1261 ctgtatccat catttttata aacatccatg gcaaacgcac ggcaatctgt ataatacgga
     1321 cttgctttaa cttgcgaata ctcaggtaat ttcatacctg cacaaccaac taaacaaaaa
     1381 cctatcgctg ctattaatac ctttttcatt acactcataa cctagaagca tcattgaaac
     1441 taatttatta aatattcatc gagtttctgg aatacagacg ttacccatct ctccaaaatc
     1501 taaaagataa taagaaaaaa tgtttaacgt accaatccat ttcatagttt catgagacat
     1561 caggcacaaa aaaacccgct cagcggcggg ttcttaaatc ttatcaacgg tagacataca
     1621 aagcccatcg ttgggaaaat cttatccata ttttttgaaa aatgcaagca tcatgtcgcc
     1681 atcttcggcg aaaatcattt atcttgtcac ttttctcaat tgtgtctctg catatgcttc
     1741 ttcctgccag cactttgtaa ccagtttatc aatgatatct gcatatcctt tgtaccactg
     1801 ataatccgtc agggcaggta ccagcttctg gacatgatgc cgcgccagtg tggttggtaa
     1861 acggctaaac cggttaccat tgcaacgccc acaaatctta taaacaggcg taccatgaag
     1921 ccgggttctt ttttcatcca ggacaatacc tttaccctta caccctctgc acgctgtgct
     1981 gacttctccc ttaccatgac aatgctgaca tagttccttc acccactctt ccttgataac
     2041 agattccccg cttctggagt gtttcaccac ctcgcgcaat acattatgaa atccagtacc
     2101 agcacaatgc tcacagcgag ccttacttgc cgcagacctg gaataatcag caaaggcaaa
     2161 attcacaagg taagggatga tctgtaaccg ggtttcttca ctcaatttat tcaatgtcgg
     2221 gttatccagt gccatcgcgt aattgagcag accttcaatc gcaaactgag gatcctgaac
     2281 accaactttt gccaggaata aggcaaaacc cagtggtgct ttcgactgca ccatcccctg
     2341 cgcagcaatt acatccgtaa ttgttaaacc accagagcct gtcgccggtg cgtcatcact
     2401 cagttttgga gattttgggg agtaatattt tggtaaggct tcaaggttca tgctcgttct
     2461 ccacttacgc cagtacgcct attgccagcg cacgatcgat aaaacgaaat atcagctcca
     2521 gctgggagcc atacttctct tcaaatgtca cggtatccgc atgcagctcg tcgtgatgct
     2581 ttctgcacaa aggcaacaca aagagatcat gcgcttttgt acccattcca ccctgaccgt
     2641 ggcctatcag gtggtgggga tcatcagctg gctttccaca acatgcgcac ggctgcgtct
     2701 taacccagcg cgtgtacttt tcattaaccc agcggcgacg tttggggcgt aacataaaag
     2761 actccggcga ctccggatcc actttcagcg ccagcacctt tttcgcctta tcctggatga
     2821 tgctggtggc aggaaccgaa ggcacaaggt cactttcccg ggtgacagac ggcacaacag
     2881 gcttcggtaa tctcagtgcc ttacgggctg cactttccgg taaggcatcc gccagatcat
     2941 tacgaatcag ccaccagcac agttccggca ttgtcacaac atgactgtca tcaaaaccga
     3001 gatccctacg cacaacagac aacacccagc gggcactgtt atccgttgcc attgattcca
     3061 gccgttccgt gaactgatcg cgcagctggt tatcgcagtg ccagcacaaa cggattgcgc
     3121 ccggagcgtg tcgcattgtt gtcatgttct cgctgtgcca gtcggaatga ggccactggc
     3181 agcctttttc acgaagtaac cagctttcaa gacattccac gccaccagca cgacggatca
     3241 ctgcctcatt gcggaacacg gcccgaacgg caggatcatc cgccagcggt tgtgatgccg
     3301 ccggaacggc accactggcg aaagatgaat aacgttccgg ctcaggctcc agcaggacac
     3361 gcccctgcat aaacaagggc atcagctctg aacctggtct gaacaatacg atccccatac
     3421 gcggggcaat ttcaggggtc agtagtgctc tcacggtcac ctcaatgaac ggtatcgagc
     3481 agctttaaca gctcagggaa tcgggattcg aagaaatgcg gctgcgtctc gcgcggattt
     3541 gcgggactgg tgatgttctt gccgaacatg cagcctttcg ccgtcagcga ccagaatttt
     3601 ttgatgttgt taatcccggt acggctgtat cgttcgcgct gctcgacgat ccccagcttc
     3661 accatctggt gatatgcctg attagccgtc aggcggatac catactgctt cagcagtgca
     3721 ctcagcgaca gcgtagggcg gcttgaacca tctggcgcat cagcaggtgc atcaatggca
     3781 tagatcggca taagttcagg aagaccagct acctttgata atttctggta tgcaccaagt
     3841 ttcgaggagt ttgacagatt tagagtcttt gctgctgatt caagcagaat gaccccggat
     3901 ttaattttgt cggatgtggt ttcttctggt gatgaattat gaagcgcatc aaaagtacgt
     3961 atcactttta agctgaatgc cgggctgatc cacattgcat atgcatagac cagctcttta
     4021 cagacatacg tcccaccatt gcgcccctga atggtgatga caggaatact acgggaatct
     4081 cccgtagttt cttcttccaa taattccaca agagccttcg tttcaggacg acgcataaac
     4141 tcgtgaactt ccagcgaacg ggaggagcga ttctcaccag cggcaagaag agcagctttc
     4201 tgaaggtcgt taagacagta gttagattcg aagtactggc gcacagaaac gccatcaatt
     4261 acaagcaact gattcattgg tttctccaca aatttttatc cacgagcggg actgcactcc
     4321 cttttcgttg atgcaggatg aacttactgc gatttttaat agttatcaag gatacactgt
     4381 tcataaatac agtatcttta acgaggtaat acccaaattt agggtgttgc tcaattccgt
     4441 taccgagttg ctaatttgca actcgcttt
//
LOCUS       DAZFYC010000223         4406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFYC010000223 DAZFYC010000000
VERSION     DAZFYC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.228"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..614
                     /locus_tag="ACHKW5_005053"
                     /pseudo
     CDS             65..614
                     /locus_tag="ACHKW5_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            791..1012
                     /locus_tag="ACHKW5_005054"
                     /pseudo
     CDS             791..1012
                     /locus_tag="ACHKW5_005054"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1545..2915)
                     /locus_tag="ACHKW5_005055"
                     /pseudo
     CDS             complement(1545..2915)
                     /locus_tag="ACHKW5_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2935..3282)
                     /gene="tnpB"
                     /locus_tag="ACHKW5_005056"
     CDS             complement(2935..3282)
                     /gene="tnpB"
                     /locus_tag="ACHKW5_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7514245.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRDRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3279..3953)
                     /gene="tnpA"
                     /locus_tag="ACHKW5_005057"
     CDS             complement(3279..3953)
                     /gene="tnpA"
                     /locus_tag="ACHKW5_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO7514246.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            4207..4406
                     /locus_tag="ACHKW5_005058"
                     /pseudo
     CDS             4207..4406
                     /locus_tag="ACHKW5_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1008 a         1052 c         1197 g         1149 t
ORIGIN      
        1 attttcagaa atcatttatt aatgaaggag ggttaaatga aaaaaactcg ttataatttc
       61 agtaatgata ttatctgtta tgagtggttc aagactggct gataatcata cgctatcggc
      121 tggttatgct cagagtaagg tacaggactt taagaatatt aaaggagtga atctgcaata
      181 tcgatatgag tgggattctc cggtcagtgt cgtcgggagt ttttcgtata tgaaaggtga
      241 ttgggcggac agtcatcgcg atgaagctga cgatttttac agacatcagg ctgatattaa
      301 atattattca tttctcgcag gcccagctta tcgtctgaat gactatatca gtttttatgg
      361 tttggtgggg atatctcata ctaaagcgaa gggagattat gagtggagaa acagtgtggg
      421 tgctgatgag tctgatggat atttaagtga gtctgtatca aaaaaaatcc actgattttg
      481 cttatgcagc tggtgttatt attaatccgt ggggcaatat gtctgtaaat gttgggtatg
      541 agggaactaa agcagatata tatggaaaac atccggtcaa tggatttact gttggagttg
      601 gttatcgttt ttaatcatta gacgggactg gataattgca cttgcgtagt gaagtttact
      661 gaatgtttgt attttaatga agtattcgac atattccctt cagacggaat atgtcgacgg
      721 ttatggtttt actggagcat gagtatgtta tggcgaaact gataaatata tcaggtccac
      781 tgaggagaga atgacgccag gggagaggag ggtggcttca cgactggaga gctttctgaa
      841 tgatgattgc tttgtctggt acgatatccc ggtgggacgg aaaaaccgac atccggattt
      901 tgtcattatt gacccggata acgggctggt atttctggag gtcaaggact ggactgtatc
      961 cacattacgt aaagcaaatc aggagcaggt tacactggaa accgacggtc ttctgaaatc
     1021 agaaataaat ccactggtac aggttcgtcg ttatgcctgt gatacggtta atgccttacc
     1081 tgctgacccg tgcttacggc agaatgacgg tcagtataag gggcggctga acctggcctg
     1141 ggcgtatggt gtggtgttta cccgcattac ccgtcagcaa ctgaaggcac tgacagggaa
     1201 taatgagaat gcggtggaaa agatttttcc ttcagcacag acaatctgtc aggatgaaat
     1261 gacgcagtct gttttacctg aggtattccg gcaaaaaatt gccgggatgt ttaccacggg
     1321 gttcagaacc cgggtaacac cccggatgag ggatattctg cgcgcccatc tgtttcctga
     1381 agtgaccgtg aaacagaact cacaaatcaa aattatggat attcagcagg agattctggc
     1441 cagaaatatt ggtgacgggc atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat
     1501 gattcttttg ttccgttgcc tttacctggc agaaaccacg tccggtagtt acgccgcccc
     1561 agtgcaacta cccggagggc gttttcacac aggttattgt cgatctccac ccagccattg
     1621 cggcagtatt cgttcagagc gtcccattgc ttcagcagat aggtgaacgc tttcgctgta
     1681 tccgagtggc gcgacagcgt gctcatctgc ccctggagcc actcatacaa cgactgcatt
     1741 agcggtaccg ttctggcttt tctgaccgcc agtcgctctt ctgccggact gccgcggatc
     1801 tcagcctcga tagcgtacag ttccccgata cgacggagcg cttctcccgt tactgtcgtt
     1861 ggatggcgga catgtacatc gtggatcttg cgtcgggcat gtgccataca agccgcttcg
     1921 gttacctgac cgctttcgta aagagcattg taacccgcat atgcatcggc ctgcaggata
     1981 cctctgtagt ccgccagatg ttgctgtggg tggatgcctt tgcggtcggg agagtatgag
     2041 aaccacaccg ctgccggcat ggtcgaaccg gcgttgcgat cgtcacgaac atagacccac
     2101 agccgtccgg tacgggtttt accctgaccc ggctccagta catttaccgg cgtgtcatcg
     2161 gcatgcactt tacccggcat cagcacatag tgcttcagtt catcatacag cgggcgaagc
     2221 tgctctccca tgatgtcaac ccagcgcccc atcgtattgc ggtgcagctc cacgccctgg
     2281 cgggcataga tttctgactg acggtacagc ggcagatgct cggcgaactt agccatgatt
     2341 atgcgggcca gcagagccgg actggcgtaa ctgcgctcga tgggttttgg tggctgcgga
     2401 gcctgaacta tacagtcgca ccggctgcag gcccgttttg ggcgaaccgt ttcgattacc
     2461 ctgaacgcgg tgttgatgat atccagttgt tcagagatgc tttctcccag cggtttcagt
     2521 ttgccgctgc agacggggca ttcggtttct gccggggaga taacctgcct gtcacgggga
     2581 agtgttgccg gaagtgcttt gcggacggga gagtctgatg ttttcggcgc tgtctctccg
     2641 gccattgagg tgagttgcaa ctgcgcctca ccaagcatgt tctggagctc ggttatacgc
     2701 gcttctgccc gtgcgatctt cttttctatc ttctcgcggc ttttctcgct gctgcgaccg
     2761 aacaacattc tctgcagttt agcgaccagc gctctgagtg agctgatctc gcgggcatag
     2821 ccggttattt caccagacag acggacgata acagcctgct gtgcgatcag cagggccttc
     2881 agttgctcga tgtcgtcggg gagtgtgttg ttcattcccc tgttttatca cgggttatat
     2941 ccggatgcca ggccgttctg tccgtttggg atgttgccat gcgatcccct ccagtagcat
     3001 ggataactga gctggcgtca ggtgcacttt cccttcccgg gttaccggcc agacgaagcg
     3061 gccccgttcc aggcgtttgg cgaacaggca taacccgtca cgatcggccc acagtatttt
     3121 caccattttg ccactgcggt cccggaagac gaagatatgc ccggagaacg ggtcatcttt
     3181 cagcgtgttc tgcaccttcg aagccaggcc gttgaagcca caacgcatat ctgtgatgcc
     3241 agcgatgatc cagattctgg taccggttgg cagcgttatc atcgggtacc tcctttcatt
     3301 tcgcggatta gcgcccgtaa catttccgga gtgagagggt caaacagttt taccacacct
     3361 gatttaagat gcagctcgca ccgtgggacg tttccgggat cactctcagg gcactcatca
     3421 ggcttgttac gccagaaggg atttgtaact ggtctggtcg gctctggcgt atcagtcaga
     3481 gccaccggga caggcatgca ttcctgtatg tcatcatcgc tcagtaagcc gtcctcgtac
     3541 tggcttttcc atttaaacag caggttatca ttgataccgt gctctctggc gatccgggca
     3601 acaacagcac cgggctgtaa tgcctgctta gccagacgga ccttaaattc acggctgtag
     3661 ctggctcgcc gttcttttcg ccatgtgcct tcgctgattt gaggctctgt taattccttc
     3721 tttctgttgg cataaaggat ggcgtcaagc tgagctaatg aaactgaatc gggcaatggc
     3781 catgcgatac cggatgcaat aaatcgctga aaaagcgtat gtattgtgga atgactgaga
     3841 cctagacgct gagcgatggc ccggatggtc agtttatctt caaatcttaa acgcagagca
     3901 tcaggcaaat aagaacggaa gcagggaata tctttttttg tctgggaatt catcgttcgt
     3961 gtccatctat atagatgggc gcgattgttg ccagacagga caattttcac aagacgtcgc
     4021 agatggggcg cttacacaac aatggcgcga gggaaagctg ctattacctt cttcagagag
     4081 cccccagcta cttcctgtga ctctcgatgc agctgccgaa cagccagaat cgctcgcaga
     4141 ggacccggaa accctcagta tcagctgttg tcaacgacgg atgactattg caccgttcgt
     4201 gacagtgtgt tgcaagcgga tgcgtacgcc gggttcaacg agctgtatcg caatggtcgg
     4261 ataacggaag ctgcctgctg ggctcatgcc cgccgaaaga tccacgatgt gcacgtccgc
     4321 atcccgtcag cactgacgga agaagccctg gagcagatcg gtcagttgta cgccatagag
     4381 gcggatataa ggggaatgcc ggcaga
//
LOCUS       DAZFYC010000224         4398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFYC010000224 DAZFYC010000000
VERSION     DAZFYC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.229"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..282)
                     /gene="yfdP"
                     /locus_tag="ACHKW5_005059"
     CDS             complement(<1..282)
                     /gene="yfdP"
                     /locus_tag="ACHKW5_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO7514247.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRL"
     gene            complement(297..608)
                     /locus_tag="ACHKW5_005060"
                     /pseudo
     CDS             complement(297..608)
                     /locus_tag="ACHKW5_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            753..1268
                     /locus_tag="ACHKW5_005061"
     CDS             753..1268
                     /locus_tag="ACHKW5_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514248.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            complement(1588..2280)
                     /locus_tag="ACHKW5_005062"
     CDS             complement(1588..2280)
                     /locus_tag="ACHKW5_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7514249.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            2378..2638
                     /locus_tag="ACHKW5_005063"
     CDS             2378..2638
                     /locus_tag="ACHKW5_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514250.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            2631..3182
                     /gene="ymfL"
                     /locus_tag="ACHKW5_005064"
     CDS             2631..3182
                     /gene="ymfL"
                     /locus_tag="ACHKW5_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO7514251.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            3358..3537
                     /locus_tag="ACHKW5_005065"
     CDS             3358..3537
                     /locus_tag="ACHKW5_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7514252.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            3527..>4398
                     /locus_tag="ACHKW5_005066"
     CDS             3527..>4398
                     /locus_tag="ACHKW5_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514253.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKR"
BASE COUNT         1156 a         1010 c         1088 g         1144 t
ORIGIN      
        1 caggcgacca ccgcgattga catacatcgg cgtttcggtg gtgtcttctt cggaaatttt
       61 cccgcggtta gttgggcgaa catatgagag tttgtgtttg attttcacac ggttctcatc
      121 aaacggttcg atttccagat tgagtgagac cttccctttg gttttcgtgt tcatcacacc
      181 ggaagcgacc tcactgagaa ctgcgccgat tttggtttca aatacgccgc cgtccagctc
      241 cccgataaat gcctgcacat cagtactgcg ttcgctagcc attttgctgc tcctcatcat
      301 atcgaccttg caaggtcggt tggtttctcc acaaaacaga gaagaacacc tgcggtggca
      361 gccgcccgga tggattgggt tatgagcccg tcgtccggtg atgctcttct ctgttttgca
      421 aaaaaggacg gtaccagccg gaagcaaggg tacaaactgg taccgccagg actacacaca
      481 gcataaagtt gtggtgccgg gtgcctcccg gtgcctggcg aaggttgcac accaggcggg
      541 tgggtatcca cagaaggtcg actgtcagcc tcaaccttaa cccgcgtgcg ctgagccgca
      601 ttcaccacaa cgctaaggat tctctctggt tgaaaatact tagctgttat gtgcctgctt
      661 ttagccacat caggcgaggt ggacctggtt attccccaac aacaaggatt cggttaatct
      721 ggatatcccc aacaacaata agagtattca atgtgatcgc tgaattaacg gcagcaatga
      781 cggctattcg tgaaacagcc cagattgcaa aactaatgaa cgaggcaaaa actcaagctg
      841 aagtaaatgc ggctattggt gagctgaact caaagcttgc gtctattcag cgcgaatgcg
      901 tgtctctcgt tgaactggtg ggctcttatc aagaaataaa tgcttctctc aaagctaaaa
      961 ttgcagaatt cgaaaacttt gaggctcaga cggaaggcta tatccttaac caacttgagt
     1021 cgggtacttt tgtatactcg aaggaggtaa tcgtgaacgg tggcagcata accatgcatc
     1081 tttgcccaaa atgttttgga caaaagatag tatcgatact tcaaccattc ccggttagcg
     1141 aagatgagct ttttcataaa agcaggtgcc tccactgtga aaataagttt ctgatgaata
     1201 aaaatccgga ttacgtatcg cctccatcca ttgaggagtt gtccagaaaa cttaacggca
     1261 atctgtagat tactactgtt gtggatatcc agattgttaa agagctaagc gtcctgtagg
     1321 gcgctttttt gttgctaacg aatcatcctg gacttcatat gccccaggcg gctacttcgt
     1381 gggcgtcctg cctgttcgtt tttgacattt actgactgct tacgacacat gcaccgtgtt
     1441 gcaaccagat tttgttgtaa tcctgtagtt ggtctggaac aaaagataaa attaaattgc
     1501 gagatatgca agtgatattt gcgtgatatg caaatttata ggtaataaaa agccaccttt
     1561 cggtggccga tggatgggat attgaggtta attatgtctc ttaagggttt gcgactgact
     1621 gattaagacc tttccaaaga ccatgaatcg gtgttcgttt tcgctagtaa ttccccattc
     1681 acggtaaatc tggttatcag aaatcaccag cagtttgtca ggaatcattt gaagtctttt
     1741 aacgtatatt ttgtcatcaa aaccaaagac atatatacca tcaccatcaa actgattgat
     1801 gctgacatca acgaagatga gatctcctgg ctcaatggtt ggacacatac tgtccccacg
     1861 aacgttgata actttgatgt gattggctgg tcgtccgccg aacattgata cagcattatc
     1921 agttctgtat tcgatggcat gaatcacatc aatgacatca ccgccctgga taaggccatt
     1981 tcccgcactg gcactgatat ccagcatttc aatacggaac acatccttca cctgcgcaac
     2041 atcctcatta ttactgtttt tatatacagt attacttttg tgggcagagg taaagagatc
     2101 agcaatatca acacctaagc tcttggcaat attactcagt gtttgttcgg taaattgttt
     2161 ttgcttaccc gtttctaagc gcgagatgtt cgccgcatct actcctatcg cttcagcgag
     2221 atcggcgatt ttcatgttct tcgcttggcg aagttgtctg actcggtttc ctatgttcat
     2281 gcgtttatta catttcttta ttgcgtgata agcaaatcaa cttgcgcaaa ataattgcgt
     2341 gaaataacat gcataacgcg caatatttgg aggacgtatg caatcaccat tacgaaatgt
     2401 gcgtaaggcg catggtttca ctttgcagca tgttgctgcg ggtgttcaag tcaatccagc
     2461 gacgttgagt cgtattgaga ggctggagca gattccatct atcgagcttg cagaacgttt
     2521 agccaatttt tttaagggtg aagtcagcga aatgcagatt ctttatccgg cacgttttca
     2581 atctagccaa aaccagaatg ggtttaaacc acaggaacag gaggtgaacc gtgggtaagc
     2641 atcactggaa agtagaaaaa cagcctgagt ggtacgtgaa agctgtcaga aaaactatcg
     2701 cagcgttgcc gggggggtac gctgaagctg ctgactggct ggatgtaaca gagaacgcat
     2761 tatttaaccg ccttcgtgcc gatggcgatc agattttccc gctgggatgg gcaatgattt
     2821 tgcaacgtgc tggtggaact cacttcattg ctgacgctgt ggcgcagtct gcaaatggcg
     2881 tctttgtgtc tcttcctgat gtcgaggatg tggacaacgc cgatatcaac caacgcctgc
     2941 tggaggtcat tgaacagatc ggcagttatt caaaacagat tcgttcagca attgaagacg
     3001 gtgtagtgga accgcatgag aagacagcaa ttaacgacga gctgtacctc tcaatttcga
     3061 agctgcagga gcatgcagca ctggtctaca aaattttttg catttcagaa agtaatgacg
     3121 cccgcgagtg tgcagctccg ggcgccgtgg cgtgtcgtga ctgtggagaa actaacgcat
     3181 gaacagttta acaacacact accgtcgctc gcaactgatt gcgcttcctg taccgggtgg
     3241 aaaagcgaag gtggagtatt gctatgcagt taatgtacca ggtggcaggg taattgtaac
     3301 ccacagcttt gcagagtggg ctgtgggtga ttttaaccgg cagaaggaga cagtcctttg
     3361 cgacaagtta accgctggtt caaagatcac tacggagtac ccgtcagagt cattcgttgg
     3421 gagccggaaa cacaacgagt tatctacctc cgcgaaggct atgagcatga gtgcttcagc
     3481 ccgctcgaac agtttcgtcg taaattcagg gaaatagagg tcggtcatga gcactaaatt
     3541 aaccggctat gtatgggatg gttgcgctgc atcaggcatg aaattatcca gcgtggcaat
     3601 tatggcccgc ctggctgatt tcagtaatga cgaaggtgtg tgctggccat caattgaaac
     3661 tattgcccgt cagattggcg cggggatgag taccgtcagg acggctatcg cacggctgga
     3721 agcagaaggc tggttaacgc gtaaggcgcg tcgccagggt aaccgcaatg cgtcgaatgt
     3781 ttatcagctt aacgttgcga agcttcaggc agcggcattt tctcaactgt cagattctga
     3841 cccgtcaaaa tctgacgcat caaaatctga cccgtcaaaa tttgatgcgt cgaaatctgg
     3901 caaaaaagcg ggttttcacc cgtcagaatc tggcggggat ccgtcagtaa aatcaaaaca
     3961 tgatccgtca gataaaaaac cttctcgtcc ggacgcttcg caaccggaca cgcagacgga
     4021 tgaacaggat tttttaactc gccatcctga tgcggttgta ttcagcccta aaaagcgcca
     4081 gtggggaacg caggatgatt tgacctgcgc acagtggctc tggaaaaaaa tcatcgccct
     4141 gtacgagcaa gccgccgaat gtgacggcga ggtggttcgt cccaaagaac cgaactggac
     4201 agcctgggca aacgaaattc gcctgatgtg tgtgcaggat ggtcgtactc acaaacaaat
     4261 ctgcgagatg tacagccgcg tcagccgcga tccgttctgg tgccgtaacg tgctcagccc
     4321 gtcgaagttg cgggaaaaat gggatgagct ttccctgcgc ttatcaccgt ccgtcagcac
     4381 gtacaccgaa aaacgcga
//
LOCUS       DAZFYC010000225         4398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFYC010000225 DAZFYC010000000
VERSION     DAZFYC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.230"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHKW5_005067"
     CDS             complement(<1..209)
                     /locus_tag="ACHKW5_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7514254.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKW5_005068"
     tRNA            complement(324..399)
                     /locus_tag="ACHKW5_005068"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKW5_005069"
     tRNA            complement(406..480)
                     /locus_tag="ACHKW5_005069"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKW5_005070"
     tRNA            complement(597..681)
                     /locus_tag="ACHKW5_005070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKW5_005071"
     tRNA            complement(690..765)
                     /locus_tag="ACHKW5_005071"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKW5_005072"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKW5_005072"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO7514255.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKW5_005073"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKW5_005073"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO7514256.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKW5_005074"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKW5_005074"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO7514257.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1125 a         1103 c          985 g         1185 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt tatttgat
//
LOCUS       DAZFYC010000226         4386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFYC010000226 DAZFYC010000000
VERSION     DAZFYC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.231"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(249..428)
                     /locus_tag="ACHKW5_005075"
     CDS             complement(249..428)
                     /locus_tag="ACHKW5_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFO7514258.1"
                     /translation="MTTTIDTNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIARELG"
     gene            complement(507..842)
                     /locus_tag="ACHKW5_005076"
     CDS             complement(507..842)
                     /locus_tag="ACHKW5_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514259.1"
                     /translation="MTVIVYGKSTFAGNAKTRRHERRRKLAIERDAICNIIDSIFGTD
                     SEEPVQEAPRKRLSLSEKAISLGSLRCQKAEEVERKQNRIYYRKPRSEMGVTCVGRQK
                     MKFGNKPLI"
     gene            complement(1286..2062)
                     /locus_tag="ACHKW5_005077"
     CDS             complement(1286..2062)
                     /locus_tag="ACHKW5_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HFO7514260.1"
                     /translation="MGNVTCSTVISKLGFECHPMSDTLLRVISPFTYYDDCEQISVFV
                     QEMSGQYRVTDYCDTLMNIESRGIHLTKKKIDLIRSSLASQGISLNDSGEISAWADES
                     SVGQVTANVIRGGILASAQTADWYAEVKDDKFEKCVISYLKSAGLGKRLALKEKVKGI
                     SGHNITVPLTLRNESRLIPPKRGFTVSLASSKGWNTAHSTVGKIVDLSQVVPDINNRF
                     VIVDSDGLTPELQQLSLLFNDTARVLPFHTRDSWIDSLVA"
     gene            complement(2050..2592)
                     /locus_tag="ACHKW5_005078"
     CDS             complement(2050..2592)
                     /locus_tag="ACHKW5_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514261.1"
                     /translation="MAKKGDFKPTQKEVDQAITCLKKVTFSGVSWTGSEGRTPIWFKL
                     DLKAFDEIGNPITGIRFMLHWRSPIVEGVDIVKLSFVMFLHDRRIYALDPYPAHNKSH
                     RNKSIVHHPDFVEVARGPHYHMYFESAGEEIALKLETNIKPDDFFGYWNYFCEALNII
                     YEGSPPLPNQDKSGQLSWEM"
     gene            complement(2690..3397)
                     /locus_tag="ACHKW5_005079"
     CDS             complement(2690..3397)
                     /locus_tag="ACHKW5_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7514262.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTFSIVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTIEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWIKSS"
     gene            3476..3702
                     /locus_tag="ACHKW5_005080"
                     /pseudo
     CDS             3476..3702
                     /locus_tag="ACHKW5_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3809..4105
                     /locus_tag="ACHKW5_005081"
     CDS             3809..4105
                     /locus_tag="ACHKW5_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO7514263.1"
                     /translation="MTQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGITKKKSPVAAGDSKQIDMQF"
     gene            4138..>4386
                     /locus_tag="ACHKW5_005082"
     CDS             4138..>4386
                     /locus_tag="ACHKW5_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7514264.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPV"
BASE COUNT         1190 a          993 c          916 g         1287 t
ORIGIN      
        1 tgctttccag tcgtaatcaa tgatgtattt tttgatgttt gacatctatt catatcctca
       61 tagataaaaa atcgccctca cactggaggg caaagaagat ttccaataat cagaacaagt
      121 cggctcctgt ttagttacga gcgacattgc tccgtgtatt cactcgttgg aatgaataca
      181 cagtgcagtg tttattctgt tatttatgcc aaaaataaag gccactatca ggcagctttg
      241 ttgttctgtt aaccaagttc tctggcaatc attgccgtcg ttcgtattgc ccatttatcg
      301 acatatttcc catcctccat tacaggaaac atttcttcag gcttaaccat gcattccgat
      361 tgcagcttgc atccattgca tcgtttgaat tgtccacacc attgatttgt atcaatagtc
      421 gtagtcatac ggatagtcct ggtattgttc catcacatcc tgaggatgct cttcgaactc
      481 ttcaaattct tcttccatat ctcacctcaa ataagtggtt tgttgccgaa tttcattttc
      541 tggcgaccaa cacaagtcac acccatttca ctgcgtggct tgcggtagta aatacggttc
      601 tgtttacgct cgacttcttc tgccttctgg cagcgaaggc ttccgagtga tattgctttt
      661 tcagaaaggc ttaaacgttt tctcggggct tcctgaacag gttcctcact gtctgtgccg
      721 aagatcgaat cgatgatgtt gcatatagca tcacgctcga tagccagctt tctgcgccgc
      781 tcatgacggc gagttttggc atttcctgca aatgttgatt tcccgtacac gattaccgtc
      841 atgatgtttt cctcatgtga aatggctttg ggggtgatgt gccagatgct gatcttctgg
      901 ttgctgtcgt tgcagctgca attcacatca ccgccaaatc catctcgttt ggtatctgtt
      961 tgcactttgt cagcgcccca tcgaagttaa agagcctgcc aatctgttcc gtttggctgc
     1021 cagcatcctg cagatggcga taataatgaa ctaatagttc gatattatca agaactataa
     1081 gtacgaaatt ttgtaactca ttaattttat tagataagat tttgatatgt aagggaattt
     1141 atttttgcaa agattgaaga ttggtctgta ggggagaaag gactgtattg ctggcgtgtt
     1201 agttcgtggg ttagcacata tgcggatgtt aaatagggga ggcgaacgtg aggtaaagaa
     1261 aacccggcgc agaggccggg tttttctagg ctaccagaga gtcaatccaa gagtctctgg
     1321 tatggaatgg caacactcgt gcggtatcat taaatagtag tgatagttgc tgtagctcag
     1381 gtgttaaccc atcgctgtca actattacaa atctattgtt tatgtcagga acgacctgac
     1441 ttaagtcaac aatcttccca actgttgagt gggcagtatt ccatccttta ctgctggcaa
     1501 ggcttaccgt aaacccgcgt tttggtggta ttagtcgaga ctcattcctt agcgttaacg
     1561 gaacagtaat gttatgccca ctaatacctt tcactttttc ctttaaggct agtcgcttcc
     1621 caagccctgc tgattttaag taactgatta cacatttttc aaacttatcg tctttgacct
     1681 cagcgtacca atcagcagtt tgggcggatg caagaatccc accacgaata acatttgcag
     1741 ttacctgtcc aacggacgac tcatctgccc acgcagatat ctctccagag tcatttaatg
     1801 aaattccttg cgaagcgagt gatgacctga tcagatcaat tttcttttta gtcaggtgga
     1861 tgccgcgtga ttcaatattc atcaatgtat cgcagtagtc tgtaacccta tactgaccac
     1921 tcatctcttg aacgaatacg cttatctgct cacaatcatc gtagtatgtg aagggactaa
     1981 taacgcgcag caacgtgtcg ctcattgggt ggcattcaaa cccgagctta gatatgactg
     2041 ttgaacacgt tacatttccc atgatagctg acctgattta tcttgattcg gtaaaggtgg
     2101 gctgccttca tatatgatat taagcgcctc gcaaaaataa ttccagtagc caaaaaaatc
     2161 atctggcttg atgttcgttt caagcttgag tgcaatttct tccccagctg attcgaagta
     2221 catgtgatag tgaggacctc gagccacctc aacaaaatct ggatggtgca ctatagattt
     2281 attacggtgt gacttgttat gcgcagggta cgggtcaagc gcgtaaatgc gcctgtcatg
     2341 aagaaacatc acaaatgaaa gcttcactat atccacccct tcaacgatag gagaacgcca
     2401 gtgaagcata aatcttatgc ctgtgattgg gttgccaatt tcatcaaaag ctttgagatc
     2461 caatttaaac cagattgggg ttcgtccctc acttccggtc cacgaaactc cgctaaaagt
     2521 tacttttttc aagcaagtaa tagcttggtc aacctctttc tgggtaggct taaagtcgcc
     2581 ttttttagcc actgattcgt atcaccatga aagttattgt taatacctat gcttcttgtc
     2641 acccaaacgt ctcttcaggc cattggttac cagctatgtg acgatgaagt cacgaacttt
     2701 taatccattc ccttgcctcg atgtcatcta ggtggcgaga ttgcttcaaa ataccagcca
     2761 catactccac ctttgctact tgatgataag gcaacgttat tggcctgtgg tcttgattga
     2821 tgcttgtaaa ttggtattct ccgtctctgt catagccaag aaccttgatc atgttgtgcc
     2881 cttcaatggt tctgacaaac acttcatcac ctgggaatac tttggtgtta ggctcaatga
     2941 gtacatattc tcctgattta attctgggcc acatgctgtc tccttttaca cgaagaccaa
     3001 aggcatctgg atcatcgcta taaatcttga gccacccatc gcgctcttcg gtcatctcga
     3061 tggcaccatc aacaccaaga attgcctcac caaccacgcg cactaaccct ttttttaatt
     3121 tgccaacaat tgaaaaagta tcttcatcat tcgctccatt taacgaagtg ccgtgctgaa
     3181 gccaaacaac atcaacgttt agaaatttcg caagcgcatt cattttttcc tggcgtggta
     3241 aagactcagc attaaaccat ttgctaacgc ctttggacga aagagaaagg gcacgggcta
     3301 tggccattcc cctaccatgt tcatcaagac cagcttcttt acaggcttgc gctagccgct
     3361 gggcgaattc tttgcgcact ttttcattct gaaccatgag tacgatacta aagcacttgc
     3421 aaaaactttc agttcaatca taatgcgtac tgaaagtacg aaaaagggta ttcctatgca
     3481 aaatcttgat gagccgatta aaggtgtcgg catccctgaa gttgcgaagg cttgtggagt
     3541 tagcgaaagg gctgtctata agtggctcaa aaacggcttc tccctaagac tgagtttttt
     3601 gggaaaacta aatacgcatc aaaaatcgaa gagatttctg gtggcaaata tcaagcaagc
     3661 gaaatgcttg aaataagcaa aaagaacctt ctggctgcat aagtaacacc gctctttatc
     3721 aatctgcacc gccgacaacg cggtaactaa ttaagcactc atcgaaagat gagtgttagt
     3781 gattatttac ctatgggaat agtaagaaat gacacaaaca agttacagca aactatcaca
     3841 gcgcgacgtt gatcgcgcag aaacagattt actcatcaac ttgtcaacgc ttacccagcg
     3901 tggtctggca aagatgattg gctgtcatga atcgaagata agcagaacgg actggcggtt
     3961 tattgcttcg gtcttgtgtg ctttcggaat ggcatcagac atcagtccga ttagcagggc
     4021 ttttaagtat gcgcttgatg gaatcacaaa gaaaaaatcc ccggtggccg ccggggactc
     4081 taagcaaatt gatatgcaat tctgagggaa ttactggatc aatccacagg ggtcattatg
     4141 acaaatacag caaaaatact caacttcggc agaggtaact ttgccgaaca ggagcgtaat
     4201 gtggcagatc tcgatgatgg ttacgccaga ctatcaaata tgctgcttga ggcttattca
     4261 ggcgcagatc tgaccaagcg acagtttaaa gtgctgcttg ccattctgcg taaaacctat
     4321 gggtggaata aaccaatgga cagaatcacc gattctcaac ttagcgagat tacaaagtta
     4381 cccgtc
//
LOCUS       DAZFYC010000227         4317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFYC010000227 DAZFYC010000000
VERSION     DAZFYC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.232"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            295..1446
                     /locus_tag="ACHKW5_005083"
     CDS             295..1446
                     /locus_tag="ACHKW5_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514265.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            1530..2401
                     /locus_tag="ACHKW5_005084"
                     /pseudo
     CDS             1530..2401
                     /locus_tag="ACHKW5_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            2619..>4317
                     /locus_tag="ACHKW5_005085"
     CDS             2619..>4317
                     /locus_tag="ACHKW5_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO7514266.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     "
BASE COUNT         1133 a         1094 c         1130 g          960 t
ORIGIN      
        1 gagaagttta cgggatgtgt atccggcaca ctatgaccgg agaatatcag tcaagcttaa
       61 aaagacaata aatacagtga gttaaataca cagagaaaac gtagtatcag agggtacatc
      121 ttacaacaga gactgaattt ccggtttttc ataaccggcg cattccggaa agtgcaggcg
      181 gcccggaggt tttctgacgc cactacctgg tagattgttc tgtacatcat gtacggaggt
      241 ttttgtttgc ttttttaaaa tctgcaaatg tgagaaaagc aggtgaggtg tattgtggga
      301 aaggaactgg atgatatctg caccagctgt ccgtatatcg atgctgtaaa acggcacaaa
      361 caacagctcg gtgccataga ggaatataca cagtggttaa aaaaagaacc acgggcatct
      421 tatttttttc tgttccagct gtatacccgc attcacaata catttttctc ccaaaagcaa
      481 caactcccgt tcactcccgg tggcactcac tgcccggaac ctgatgtcac ccttcgggac
      541 ttaacgcttt cccctggcta ccacagtgat tatgcccctc aacccattcc ggagatggac
      601 agttctgctg ttgttccccc aactaatgaa aacacctcgc ctccggaaga tactcccgac
      661 aacactccgg ccggaggcaa taccggacaa gcggaaaaga ccagaaacgc aggactcaca
      721 cccattcctg aaaaacgttc aggaatgccc ccggaacatc tgcggtttgc cacggggttt
      781 ccatcgcaac cgaagattgc cggtcccaga gaaaagccga tgcgcaccgt gcatccggat
      841 aaaatctacc gtgaaattat atggttctgc tccagttacc tgctgaaatc cggaccagag
      901 gctacgagga cgattatcaa ctcagtattc tctgaatggg ccagcataaa caatgattac
      961 ccctccccct tttcgtgggt ggacagcagg gacagtgaac agtgtgactg gttatggaac
     1021 gccatgcagg tccggtgtgt gggaaccccg ctgaatcccc ttaccccgga gcagaaatac
     1081 tggtttgcct gcgccacgtt tgataactgg gagggctgga atgagcaaca ggtacagttt
     1141 ttactggaaa gtaatcccag acggaacaga gcgaagttta cgcaggtctc cttccaggcc
     1201 cccaggattc agcataaagc gattcttctt gatgagctga aaagtgcccg ggagcaacaa
     1261 aaaaggcgtg atgaacgtgc tgatggttcc gtccctctga aactgtccgg aaaaatccac
     1321 aaacagcttg aaagtattgc ccgaagtcgt ggtgtcctcc caaaaaaact gctgaatgaa
     1381 atgattgagc aggcgtacca tgatttggtg gcaaccaggc agaacagcca aatcgattcc
     1441 agataatttc agacagatat tatttccgtg aatcccccgc cacctttccg gtgcgcgggg
     1501 ttttgtcttt tttcaccggg aatacatgta tgaatccgtc tgatgccatt gaggcaattg
     1561 aaaaaccgct ctcctccctg ccttactcac tttcccgtca catcctggaa catctgcgca
     1621 aactcaccag tcacgaaccc gtgcctggca ttatgggtaa aagcgggccc gtaaatcctc
     1681 actctgtaat gcgctgtttc agggggaggt caccccggtc agtgatgttc acgccggcac
     1741 ccgggaagtg cagcgcttcc gtctgaatgg ccatggtcac agcatggtta tcactgacct
     1801 gcccggggtg ggcgagagcc gggacaggga tgcagagtat gaagccctgt accgtgacat
     1861 tctgctcgaa ctggacctgg tactgtggct gattaaagcc gatgaccgtg ccctgtctgt
     1921 ggatgagtat ttctggcgac acatcctgca ccggggacat cagcgggtac tgtttgtggt
     1981 gatgcaggcc gataaaacgg agccctgcca tgaatgggat atggccggta ttcagccttc
     2041 tcccgcagag gcacagaaca ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc
     2101 cgtacatcgg gttgttgccg tatcggcccg caccggctgg gaactggata cgctggtcag
     2161 tgcgctcatg acagcacttc ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga
     2221 cgagctgcgc acggagtctg tccggtctca ggcccgtgaa cagtttaccg gtgcggtgga
     2281 ccggatattt gacacggcgg agagcgtctg cattgcctct gttgcacgca cggttctgcg
     2341 cgccgtccgt gactcggtgg tctctgttgc ccgcgcggta tggaactgga ttttcttctg
     2401 aacccggtgg tgatgtacca ggctgttgtt acagattttt aactccatga aaacactatg
     2461 caaacgcagc catacccggg ttgtcccctt gcgttgtgtt tctcaagtta atagtatgca
     2521 tcgatcgtta ctatcatatc gatcgttata agcgatcgtt tgtagtaaat gacctcctcc
     2581 gtggttgtca tgaaaatgcg tcttaggaag gttaaataat gcacgaaaag aacatcgccc
     2641 tgctttgtga tgaagccgac cgacttttgc aactgaacat taatctgctc cggcaaatgg
     2701 ttgaggagcc agatgtgtta tctgacagta agaacgaaaa cagactgctt tttgataaac
     2761 agaaagcact gaaaagaatt gaggagctgg agggcgaaca aatcaaaacc gcccgcaggg
     2821 agatggtgct ggctgttgtc ggcacgatga aagcaggcaa atcaaccacc atcaacgcca
     2881 ttgtggggca ggaaattctg cctaaccgta accgccccat gacctctgta ccgacgctca
     2941 tccgccacgt tcccggaaaa actgagccgg ttctccatct ggaacatatt cagcctgtcc
     3001 gcaatttatt aatcacactg caggaaaaac tcgccacccc ggcaggacag caggtcgcac
     3061 agaccctgca gcaaaccggg gatacccgcg aactgctgga tattctgacg gatgatggct
     3121 ggctcaaaaa tgaataccac ggggaggagg aaatctttac cggactggca tcgttaaacg
     3181 atctggttcg tcttgctgcg gcaatgggga ctgaatttcc ttttgatgaa tacgcagaag
     3241 tgcagaaact gccggtgatc gacgtggaat tcagccatct ggtggggatg gatgcatgcc
     3301 agggaacact cacactgctg gatacccccg gccctaatga ggccggacaa ccgcagatgg
     3361 aagtgatgat gcgggatcaa ctgcagaaag cctctgcggt tctggctgtg atggattaca
     3421 cccagatgaa ctcaaaagcg gatgaagacg tccgtaaaga gcttaatgcc attgctgacg
     3481 tatcagccgg ccgcctgttt gtactggtca ataaatttga tgagaaagac cgcaatggcg
     3541 atggggcaga tgccgtacgc cagaaagttc cggcaatgct gaacagcgat gtgctgcccg
     3601 cctcccgcgt ttatcccgga tcctcacgcc aggcatacct ggctaaccgt gcgcttcatg
     3661 agttacggaa aaacggaacc cttcctgttg atgaagcctg ggtcgatgat tttatcagag
     3721 gtgccttcgg ttgcatgaaa aaagaatacg tctgtaaaga cagtgaactg gcaactgaag
     3781 gggcgacaga cctgtgggaa ggctcactta tcgatcaact gataacggaa gtcatacaga
     3841 gctcacattc cagagctgcg gcactggcgg ttgactctgc cgccgcaaaa ctgatgcaga
     3901 atgcagaaaa tatcagtgaa tacctgttgt tacgccatca ggggctacag caaagcattc
     3961 agtcactgca gtcgcatatc accagcctgc ttgcggatat ccgggaaatc gcggactgtc
     4021 aggagcagat gaccactgat gtcagaatgg ccatggagga gatcgatact aaaacccggg
     4081 aattactgac gggggtctgc acctcactgg aagaggagct gaatgactat ttcagaagcg
     4141 gtaaacgcaa agaacagcaa atgctggagg aagaagacgc agaacaacga aggtctcagt
     4201 ccggattatg ggggaaaatt tctcaatggt ccggtatcaa caaccagggc cgggaggatt
     4261 acaggaaacg agattttgcc ccggacagcc ccgaaataaa attcagtgat cgcaggg
//
LOCUS       DAZFYC010000228         4276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFYC010000228 DAZFYC010000000
VERSION     DAZFYC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.233"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..504
                     /locus_tag="ACHKW5_005086"
     CDS             <1..504
                     /locus_tag="ACHKW5_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7514267.1"
                     /translation="GFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKV
                     YARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGR
                     IMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGF
                     LSINKLS"
     gene            complement(524..844)
                     /locus_tag="ACHKW5_005087"
     CDS             complement(524..844)
                     /locus_tag="ACHKW5_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO7514268.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            951..1124
                     /locus_tag="ACHKW5_005088"
     CDS             951..1124
                     /locus_tag="ACHKW5_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO7514269.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            1195..2118
                     /locus_tag="ACHKW5_005089"
     CDS             1195..2118
                     /locus_tag="ACHKW5_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO7514270.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFAKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIREKVI
                     QYQEECDDVLYEYWTKGHVVNPRKAKKALLGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKSTNEFSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTR
                     DIDINEPDGPTNLSAWVRLKNKELPPSLHHY"
     gene            2173..2910
                     /locus_tag="ACHKW5_005090"
     CDS             2173..2910
                     /locus_tag="ACHKW5_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7514271.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYFPCVNISAEPEAY
                     FRIAPEDWLQAEMQGGIVALVHSHPCGLPWLSEADRRLQIKSALSWWLVCRGDIHKFR
                     CVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDEWWRNGQNLYLDNMEATGF
                     YRVPLSSAQAGDILLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTH
                     SLWRHREWHASAFTGICNDLAAASTFV"
     gene            2874..3449
                     /locus_tag="ACHKW5_005091"
     CDS             2874..3449
                     /locus_tag="ACHKW5_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514272.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLNEGWYQVRISG
                     RDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGIFQAVLGAALIATAIWMPGLSIV
                     ASNLMFSLGAGMVLGGVAQMLAPKAKTPKVSATDNGKQNTYFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVASQEISTADEGDGGQVVVIGR"
     gene            3522..3860
                     /locus_tag="ACHKW5_005092"
     CDS             3522..3860
                     /locus_tag="ACHKW5_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514273.1"
                     /translation="MNKTILFCTIIALTGCKSLDYVKSGKPVMEGNSLKNIDELSGCI
                     SRQWAGNGTPITSIPIENGVSLLVPQAMGGYDVVLDIKKAGNGSSFTLYERVPALTPK
                     IFADSVNACK"
     gene            3927..>4276
                     /locus_tag="ACHKW5_005093"
     CDS             3927..>4276
                     /locus_tag="ACHKW5_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7514274.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNANISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFG"
BASE COUNT         1062 a          965 c         1156 g         1093 t
ORIGIN      
        1 ggttttgaac tgaatggcaa aggcaccagt acgcgcccca cgctgacggt ttctaacctg
       61 tacggtatgg tcaccgggat ggcggaagat atgcagagtc tggtcggcgg aacggtggtc
      121 cggcgtaagg tttacgcccg ttttctggat gcggtgaact tcgtcaacgg aaacagtgac
      181 gccgatccgg agcaggaggt gatcagccgc tggcgcattg agcagtgcag cgaactgagc
      241 gcggtgagtg cctcttttgt actgtccacg ccgacggaaa cggacggcgc tgtttttccg
      301 ggacgtatca tgctggccaa cacctgcacc tggacctatc gcggcgatga gtgcggttat
      361 cacggtccgg ctgtcgcgga tgaatatgac cagccaacgt ccgatatcac gaaggataaa
      421 tgcagcaaat gcctgagcgg ttgtaagttc cgcaataacg tcggcaactt tggcggcttc
      481 ctttccatta acaaactttc gtagtaatgg attatgccca ccgtcaggtg ggttttttgt
      541 ttagcagttc tctcaacttt tcgttctgct ctctgaactt ttcctgtatt tctttttgca
      601 tggcgatcac ttgggcttga agttgaacta gcgcatcaac atttagcgga accgagacgc
      661 tgttgatttt gtctgctatt tccccaagag tattttctgc attcaaggca tcttccagta
      721 tctgaacaat ctctgagttc atagagcgcc cgtttcgttt ggctcgttca gctatagcat
      781 ctcgcattcc gtcaggaaaa cggaggttga acttgtcgta gtcttttact tgtttttcgg
      841 ccattgcaaa tctctcaaaa aaatcatggt gccatattgc catacgattt caatggtggc
      901 attatggcct tcatggcgtc actttggcac caaataaagg agattagata atgcaagata
      961 cacttttcac tgagcgcaaa aatatcaaac tcaaccttcg ccttccatca cggctgaatg
     1021 aagaccttcg ccgcctggcg gaaatggact gtatatctct gaactctgca attgttcgtt
     1081 tgctggcaaa aggtgttagg gaagaggtgg cgaatggtcg ctaaaaacag cgaagcccca
     1141 atggctgcaa ccattgaggc ttctaaatta ccagttaacc acgagaaaac tgatatgact
     1201 agtttagcaa ttgcagatcg cacaatcaat gttccattcc acggaacaaa tctctttttg
     1261 gttggaatta acaatgagcc ttatgttcct atgaagcctg ttgttgaagg tatggggatg
     1321 gtttgggctg ctcaatttgt taagttaaaa cagaggtttg ccaaaggtat ttcggaaatc
     1381 gaaatacctt ctgctggcgg taaacagtta atgacatgtc ttgcctttcg taaatttgcg
     1441 gcttggcttt caagtattca accaaacaaa gtccgccctg aaatccgcga gaaggtaatc
     1501 cagtatcagg aagagtgtga cgatgtgctc tacgagtact ggactaaagg ccatgtggtt
     1561 aacccgcgca aagctaaaaa ggcattgctg gggaaaatca ccactgaaca gcaggaagcc
     1621 attaaacaac tcgtcatgag tcgcggtcag tctctgccaa aagaaaaaca ggcgaaggcg
     1681 atgattacca tgtggtcgtc actgaaatct cattttgggt gttcatacaa agaaatcagc
     1741 gaggagcagt ttaccgaagc tctgtcactt gctgctcgcg ttccgcttga aggagagtta
     1801 atcggcaaac aagagaagag caccaatgag ttttcagcga aagaagcaaa cagccttgta
     1861 tggctatggg attatgccaa ccgctcacag gcattattcc gcgaactgta tccggcgcta
     1921 aagcaaattc aatcaaacta ttctggcagg tgctacgact acggccatga gttctcgtat
     1981 gttatcggaa tggcgagaga cgttttaatc aatcacacgc gagatattga tattaatgag
     2041 ccagacggac caacgaatct ttctgcatgg gtaagactta agaacaaaga attacctcct
     2101 tcactgcatc actactaaca gattgccaac gaaatgaccc agcttcggct gggtttttta
     2161 tcaggagttc tcatgctcta tagcaatata ttggcgcacg cccggcgatg tgcgccagcg
     2221 gagtcgtgcg gcttcgtggt gagaacgccg gagggggaaa gatattttcc ctgcgtgaat
     2281 atctccgcag agccggaggc gtattttcgt attgcaccgg aagactggct gcaggcagag
     2341 atgcaggggg ggattgtggc gctggtccac agtcatccct gtggtctgcc ctggctgagc
     2401 gaggcagacc ggcgtctgca gataaaaagc gcactgtcct ggtggctggt ctgccgggga
     2461 gacattcata aattccgctg tgttccacac ctgaccgggc ggcgttttga gcacggggtg
     2521 acggactgtt acacgctgtt ccgggatgca taccatctgg cggggattga gatgccggat
     2581 tttcaccgcg aggatgagtg gtggcgaaac ggtcagaacc tgtacctgga caatatggag
     2641 gcaacgggct tttaccgggt gcccctgtcc tctgcacagg cgggcgatat tctgctgtgc
     2701 tgttttggtt catcggtgcc gaatcatgcc gccatttact gcggcgacgg tgagctgctg
     2761 caccatattc ctgaacaact gagtaaacga gagaggtata ccgacaaatg gcagcgacgc
     2821 acacactccc tctggcgtca ccgggaatgg cacgcatctg cctttacggg gatttgcaac
     2881 gatttggccg ccgcatcgac cttcgtgtga aaacgggggc tgaagccatc cgcgcactgg
     2941 ccacgcagct tccggcgttt cgccagaaac tgaatgaggg ctggtatcag gtgcgcattt
     3001 ccgggcgtga tgcaggcgaa aacgaattat ctgcccgtct taatgagccg ctggcaaatg
     3061 gtgccgtgat ccacatcgta ccgcgtctgg cgggtgccaa aagtggcggt atttttcagg
     3121 cagtgctggg tgcggcgcta attgctacgg caatctggat gccggggctt agtattgttg
     3181 caagtaatct catgttctct cttggggcag gtatggtttt aggcggtgtt gcgcagatgc
     3241 tggcaccgaa agccaaaacg cctaaagtgt cagccactga taacggtaag caaaatacct
     3301 atttttcctc attggataac atggttgccc agggcaatgt cctgcctgtt ctgtacggtg
     3361 aaatgcgcgt ggggtcacgt gtggcatctc aggagatcag cacggcagat gaaggggatg
     3421 gtggtcaggt tgtggtaatt gggcggtaat attatttact catgttctaa ctgatttaat
     3481 atttatatcg aacactgata attattctgt tggttagcta tatgaacaaa acgattttat
     3541 tctgcacgat tattgcctta acaggatgta aatctttgga ttacgtaaaa tccggcaagc
     3601 ctgtaatgga aggtaattca ttaaaaaata ttgatgaatt gtcaggctgc atatccagac
     3661 aatgggctgg taatggaaca cctataacat ccattcctat tgagaatggg gtaagccttt
     3721 tagttccaca ggctatgggt gggtatgatg ttgtgcttga tatcaaaaaa gcaggaaatg
     3781 gcagtagttt tactctttat gaacgcgtac cagcattaac gccaaaaatt tttgctgata
     3841 gtgttaatgc atgtaaataa tagttaatcc tgccgtaact catgagccgc cttttgggcg
     3901 gctttgttgt ttatggagtg tgaggaatgg gtaaaggtag cagtaagggg cataccccgc
     3961 gcgaagcgaa ggacaacctg aagtccacgc agctgctgag tgtgatcgat gccatcagcg
     4021 aagggccggt tgaaggtccg gtggatggat taaaaagcgt gctgctgaac agtacgccgg
     4081 tgctggacag tgaggggaat gccaatatct ccggcgtcac ggtggtgttc cgggcaggtg
     4141 agcaggagca gacaccgcct gagggctttg aatcctccgg ctccgagacg gtgctgggta
     4201 cggaagtgaa atatgacacg ccgatcaccc ggaccatcac gtcggcaaac attgaccgtc
     4261 tgcgttttac tttcgg
//
LOCUS       DAZFYC010000229         4236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFYC010000229 DAZFYC010000000
VERSION     DAZFYC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.234"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..128
                     /gene="ykiE"
                     /locus_tag="ACHKW5_005094"
     CDS             60..128
                     /gene="ykiE"
                     /locus_tag="ACHKW5_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO7514275.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            403..501
                     /locus_tag="ACHKW5_005095"
                     /pseudo
     CDS             403..501
                     /locus_tag="ACHKW5_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            817..1098
                     /locus_tag="ACHKW5_005096"
     CDS             817..1098
                     /locus_tag="ACHKW5_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO7514276.1"
                     /translation="MTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAV
                     NALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(1436..1567)
                     /locus_tag="ACHKW5_005097"
                     /pseudo
     CDS             complement(1436..1567)
                     /locus_tag="ACHKW5_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1849..3207
                     /locus_tag="ACHKW5_005098"
     CDS             1849..3207
                     /locus_tag="ACHKW5_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO7514277.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEEEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(3329..3406)
                     /locus_tag="ACHKW5_005099"
                     /pseudo
     CDS             complement(3329..3406)
                     /locus_tag="ACHKW5_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            complement(3446..>4236)
                     /locus_tag="ACHKW5_005100"
     CDS             complement(3446..>4236)
                     /locus_tag="ACHKW5_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7514278.1"
                     /translation="QSEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALHGYVMTDGK
                     LHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVRFAYSPDRKGIHPQTHL
                     ACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQ
                     LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGERGALLYS
                     LIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         1128 a         1014 c         1036 g         1058 t
ORIGIN      
        1 tgtttccaat agacattttt aacatgttgt ttttctaagt gttataaggt aggtataaaa
       61 tgggatggag cctctgcttc tggcatgtgt cggtcagaat gactcatgat gtggtctgct
      121 attattgaca tcctcactgc cctaaaggat ggggatttcg gtaatgatct tacccagcaa
      181 tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca agacaaaaca
      241 gtttcaacca gtaaaaaaac gtaaacacca ttcgcatgaa tttcgtagtt aagatctgaa
      301 gcttgcagaa tgcatcggtg ttactgccgg agtccgtgaa cttagtctgc atgaatcacc
      361 acagctttac atctgtcgca gtaaacagca aaatcagcaa acttacttaa ttatatcgag
      421 tgcgattaca atcgctggcg atggtacagt tggtgtggcg gtctcagtcc ggaacaattt
      481 gaaaatcaga atctcactta aaacggcatc cattttacaa aagatggccg tctgtgtatt
      541 tataatgaat accttctaca tgtttagagt gcataatcca cgacaaggcg atatcctaaa
      601 aattagctag caggcaactt tgatggcata gataatgttt ttagtaaaaa agagttgact
      661 taatataata cagcctcttc ctacataaga aggaatgtgc tcacaaaaaa aaaccattag
      721 tctgcttcct gttcttacaa taatcagtat tatgtatttt atttatatag caaaatatgc
      781 ttgttacacc tgtcataatt aggcatggta aaattattga ctgaaccctg ggggagaaag
      841 atgcacagtg attattataa tatggttttt ggtgaaaaat tggccaatat tttatatgaa
      901 gctaactctc aattttttta cgagagaaat gtcatagaag aggcagtcaa cgctttattc
      961 tgtgagcgtg aaataataaa caacaagaac ataataaaga agctgatgtt ctttttgtca
     1021 gatgttaatc acaccaagaa agatgttgtt cagagcgcac ttaatattat cattgacatt
     1081 acatcaggtg atatttgaga ttttacataa aagataatct ctcaaggagt cttatctgaa
     1141 aacgaaatat atagtccctc tcgttttgta atatcgattg gctttgttga atcggttacg
     1201 taccccggag ccggtcatat tttcaggtaa tacacacgct ttcagacatt tcggctttcg
     1261 tcattttggt atcatgggca tagcttctgc tacctgacca tcataatcac gcagcttcag
     1321 tgaagcagtg accacctgtt taacccggtg tatcgttatg atcttatcca gcaataatgg
     1381 acacgcggct aagtgaaccg ccccgggttt cctggagagt gttttatctg tgaactcagg
     1441 ctgccagatc atcgtttccg atggaagcat aataagcttt ttctgcttct gccggtggga
     1501 tatggccctg cctttccagc aatcgtcgat tgttatacca gtccacccac gtgagtgtgg
     1561 tcagctcatc atactgacac gtgcattacc cctaccgcga taatgcaacc gtacaatata
     1621 gggcgtttcc taaacgcagc cctcatcact ccaatccaac aagacacagc atttttgatc
     1681 acgaagaaag acaaataatt tccgtaaatg tttccgttgt tacaaataac aacagtgttt
     1741 tacatttctt tggcctcagg cccgcgcatg ttgccattcg atgtcatttt cctgactcgt
     1801 ttcttcttta tggtgtagtg tgatcttcac tttatggatg ctaacaatat gaaaagaaaa
     1861 attattcccg tgctaatcgg ttgtacgctc tctttctctg ccctggcggc acaacctacg
     1921 gctgagcgct atgtcgtcag ttttccagaa ggcacgcacg tgaactacgc cggcgcattt
     1981 gccagtgcat tcccgaacgg ccttccggtt gggataggtt ctggcctgct gttcaccggt
     2041 aaacagggcg atgccctgac attcgcaacc attaccgatc gcggtccgaa cgcggattca
     2101 ccgaaagagg ggaaaaacga aactaaaatt ttcgttacgc cagatttcgc gccgctgctg
     2161 atgacgattc gtgtacaaaa cggcaaagcg gaggccatcg atccccgtcc tctgcatgac
     2221 gataaaggcg cgatcaacgg tctgccgctg gcaagcgacg tgattggctc caccaatgaa
     2281 gtggccttca gcgacacgtt acatagactg aagggggata atcgcgggct ggatacggaa
     2341 gggatcactc cggatggtaa aggaggttac tggctgtgcg atgagtatgg cccgttcctg
     2401 attaacatcg acagtaaagg gaaaatcctg gcaatccacg gtccgcaggc ggcggaagag
     2461 gaaaaagcga ttgcgggcgg tttgccaaat atcctcaaat ggcgtcaggc gaaccgcggc
     2521 tttgaaggcc tcacccgtat gccggacggg cgtattattg tcgccgtgca aagtacgctg
     2581 gatatcgacg ctaagagcaa gaaaaaggcg ctgtttacgc gtctggtgag cttcgatccg
     2641 gcaagcggaa aaaccgcgat gtacggttat cccatcgaca gcgcggccta cagcaaaaac
     2701 agcgacgcta aaatcggaga catcgtggcg ctcgataacc agcacatcct gctgatcgaa
     2761 cagggaagag ataaaaacaa cagaatgcgt aacctgattt acgaggtcga tctcaataag
     2821 gcgagcgacc tgtcaggctt cgacaagccg ggtgagtatc cggagtttga tgatgagaaa
     2881 accctgtcac agcgcggcat tacccttgcg cagaaaacgc aggttgttga tctgcgctcc
     2941 ttgggctggc agcaggagaa ggctgaaggg ttggcgctga tcgacagcaa aacgctggcg
     3001 gtggcgaacg ataacgactt tggcgtgaag gtggcgatgc aacatccggt cgagggcaag
     3061 acattcaagg attatcgggt gaacgcggaa ggaaagctga cgcttgacga caagcaggtt
     3121 gaaacgaccc tcagagtgaa gccactggag aaaccggagt ctgacagtga actgtggatt
     3181 gtgacgctgc cggaagcgtt gaaataagtt tataaggtac ggacagcagt atcgcttagt
     3241 ttttcaaatt catgtatgtg aacaatattg gggttccggc gtagaccccc aatgaaagcg
     3301 tcaccacgtg aaaacatgcc tgcacggttt acacgtcatc ccatggtggc agccggttaa
     3361 ctttcctgcg ccaccagtgg aaaaaggcca taagcagtcc gaacaaaagt aagcgtacag
     3421 cgagggccgt attgacgggg atgtgttatt cagctggcag tgctatgcgc cacggaagca
     3481 gttcgctgac ccggttgacc ggccagtctg ctatgacgcc aggcacatgg cgaaggtagc
     3541 tttctggatc cacgtcattc agtttgcacg tcccgatcag gctgtacagt agcgctcccc
     3601 gctcaccacc atggccagag ccgaagaaca gggtcttcat cttttcacgc aaccagcttt
     3661 ccagggattt caacagcggt ttcgtttttc gctgacgttc agcaagccgc tgctctgccg
     3721 gcattcccct tatatccgcc tctatggcgt acaactgacc gatctgctcc agggcttctt
     3781 ccgtcagtgc tgacgggatg cggacgtgca catcgtggat ctttcggcgg gcatgagccc
     3841 agcaggcagc ttccgttatc ccaccattgc gatacagctc gttgaacccg gcgtacgcat
     3901 ccgcttgcag cacaccgctg aagcaagcaa gatgagtctg cggatggatg ccttttctgt
     3961 ccgggctgta agcgaacctc actgcaggtg ccaacgctga cccggcattg cggtcatcac
     4021 gaacatacgc ccacaaccgc ccggtcttcg tcttcttatt acccggcagc agtacctgga
     4081 ccggggtatc atcggcatgg agtttgccgt cagtcatgac atagccatga agcgcctctt
     4141 ccagcggaga cagcagccgg cagcatgcat ccacccagcc cgacagcagt gaacggctca
     4201 gctccacacc ttgccggccg tatatttctg actggc
//
LOCUS       DAZFYC010000230         4210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFYC010000230 DAZFYC010000000
VERSION     DAZFYC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.235"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            288..896
                     /locus_tag="ACHKW5_005101"
     CDS             288..896
                     /locus_tag="ACHKW5_005101"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO7514279.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            994..2385
                     /gene="selA"
                     /locus_tag="ACHKW5_005102"
     CDS             994..2385
                     /gene="selA"
                     /locus_tag="ACHKW5_005102"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO7514280.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2382..>4210
                     /gene="selB"
                     /locus_tag="ACHKW5_005103"
     CDS             2382..>4210
                     /gene="selB"
                     /locus_tag="ACHKW5_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO7514281.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT          941 a         1072 c         1234 g          963 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat
      121 aagaaagcct taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac
      181 catgataaat atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc
      241 ccatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg
      301 gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa
      361 ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc
      421 cgttaggaaa agtgccggtg ctggtgaccg aagagggcga atgttggttt gattcgccga
      481 tcatcgctga atatattgaa ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc
      541 tggagtcgtt gcgggtgcgc aaaattgagg cactggcgga tggcattatg gatgccgggc
      601 tggtatcggt gcgtgaacag gcgcgtccag cggcgcagca gtctgaagat gaattgttac
      661 gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca
      721 cactcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgcc gtcggatatc
      781 tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg cccacactta gtcaaactgg
      841 tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg
      901 cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc
      961 cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat
     1021 agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat
     1081 acttatggtc acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa
     1141 gtgattcgtg gcagccagac gctgcctgcg tggtgtgaaa actgggcgca agaagtcgat
     1201 gcccggttga cgaaagaagc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc
     1261 gtgctgcata ccaaccttgg acgagcttta caggcggaag ccgcggtgga agccgttgcg
     1321 caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgccgg acgcggacat
     1381 cgcgatcggg cgctggcgca actgctgtgc cgcattacgg gggcggaaga tgcctgtatc
     1441 gttaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag
     1501 gtggtggttt ctcgcggcga actggtggag attggcggcg cgtttcgtat tcccgatgtt
     1561 atgcgtcagg caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat
     1621 gattatcgtc aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac
     1681 tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa
     1741 gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag
     1801 tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg
     1861 agtttctccg gcgacaagtt gttaggcggg ccgcaggcag gaattattgt tggtaaaaaa
     1921 gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg
     1981 accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa
     2041 aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa
     2101 cgtttacagg ccccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca
     2161 tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta
     2221 acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt
     2281 gaattgccag tgccggtgat tggtcgtatt tatgacggac gattgtggct ggatttacgc
     2341 tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg
     2401 gacacgttga ccacggcaaa acaaccttat tgcaggcgat tactggcgta aatgctgacc
     2461 gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc
     2521 agccggatgg tcgcgtgcct ggttttatcg acgtacccgg tcatgaaaag tttctttcca
     2581 acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg
     2641 gcgtgatggc acagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc
     2701 tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttaat gaggttgaac
     2761 gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg
     2821 cagcaaccga aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttgccggaac
     2881 gcgagcacgc cagccaacat agtttccgcc tcgctattga ccgcgcattt accgtaaaag
     2941 gtgccgggct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac
     3001 tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgcg caaaaccagc
     3061 caacagaaac cgccaatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa
     3121 aagagcagat taaccgtggc gactggctgc ttgccgatgt gccgccagag ccgttcacac
     3181 gggtgattgt cgagcttcaa acccatacac cgctgaccca gtggcagccg ctgcatattc
     3241 accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc
     3301 tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct
     3361 cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta
     3421 aacgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg
     3481 atgcgttatc tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc
     3541 gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg
     3601 gttatagctt gttgaatgcg ccggttgccg ctcgctggca acggaaaatt ctcgatacat
     3661 tagcgactta tcatgagcaa catcgcgatg aacctgggcc tgggcgcgaa cgtctgcgac
     3721 gtatggcgtt gccaatggaa gatgaagcgc tagtactgtt gctgattgaa aagatgcgcg
     3781 aaagcggcga tatcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct
     3841 tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt
     3901 ggtgggtgcg tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc
     3961 tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta
     4021 acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa
     4081 cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc
     4141 tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg
     4201 acgcattatt
//
LOCUS       DAZFYC010000231         4207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFYC010000231 DAZFYC010000000
VERSION     DAZFYC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.236"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..149)
                     /locus_tag="ACHKW5_005104"
     CDS             complement(<1..149)
                     /locus_tag="ACHKW5_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514282.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVV"
     gene            complement(151..429)
                     /locus_tag="ACHKW5_005105"
     CDS             complement(151..429)
                     /locus_tag="ACHKW5_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514283.1"
                     /translation="MVHYKKLNNSDNSAVFASRHGRCSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISARLPYSERPPRPSRTFQQRIWR"
     gene            complement(495..662)
                     /locus_tag="ACHKW5_005106"
     CDS             complement(495..662)
                     /locus_tag="ACHKW5_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514284.1"
                     /translation="MNDKELIAALSIPGNYEVIVLGNGEFIVMPLPSDVILISKESHA
                     DSVSYFSIKRD"
     gene            complement(941..1096)
                     /gene="hokD"
                     /locus_tag="ACHKW5_005107"
     CDS             complement(941..1096)
                     /gene="hokD"
                     /locus_tag="ACHKW5_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7514285.1"
                     /translation="MKQQKAMLVALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1276..2397)
                     /locus_tag="ACHKW5_005108"
     CDS             complement(1276..2397)
                     /locus_tag="ACHKW5_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514286.1"
                     /translation="MELFDSIGNYRGDCLKLARQAKGVTGEELATALGVTRQFINKLE
                     RGYPPSSQMLTALADYLDVKESFFFTGRKSPLDSENCHFRSKRSRTQSLTNSILARAE
                     ILEQFVKCLETEIVLPELNFPDTSGLPLNSDEDIERIAEACRRYWGLGLGPISSMVNF
                     VESIGVVVAHVSGVDDRIDAFTINSKRPLIIRNSAKKSVCRFRSDLGHELGHLVLHEG
                     IITGDKITEHHADYFSSAFLVPRVSFVNEFPKMRGSHLDWNALINFKERWKISLRMCI
                     YRATVLGLITPQQMRTGFIHLNKRGTIKGEMGDELIPEEKPRLLSCAVELLDISSWKQ
                     ILDMSGVRERFVSKMFGIRRTHDDISSNIVPLYRYKDFG"
     gene            complement(2384..3034)
                     /locus_tag="ACHKW5_005109"
     CDS             complement(2384..3034)
                     /locus_tag="ACHKW5_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514287.1"
                     /translation="MPSEQNLQPCFEINPCLDEKSVINLLQQFANVLGELWFHRHEYD
                     DNWINGCRAYGWIKNFLVKNHKKLKGVAILSTPLNFVFTLNRVPLQFVTDKLDSPKKK
                     HRLCKNDVECRQLSLFEDDVNFEHDVTWRVLAEKLPSANKKDEDIEPPTWLLALIGFD
                     SSNNVVSRYELVSKVHAPVQAANTDVLPEAVETPPLNLQRRVKNAKKADSDKYGTI"
     gene            complement(3189..3383)
                     /locus_tag="ACHKW5_005110"
                     /pseudo
     CDS             complement(3189..3383)
                     /locus_tag="ACHKW5_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3416..3838)
                     /locus_tag="ACHKW5_005111"
     CDS             complement(3416..3838)
                     /locus_tag="ACHKW5_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7514288.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRQLEEKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRANLKKTSVDDPDLIWSLPDG
                     EIRRYDRRQNIICSECRKSEVMQRALAFYQGNFQEVAQ"
     gene            complement(3854..>4207)
                     /locus_tag="ACHKW5_005112"
     CDS             complement(3854..>4207)
                     /locus_tag="ACHKW5_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7514289.1"
                     /translation="PGDNLPAEPKAASVAETDGKAFPQPACVALPVQEAATQEDIKTE
                     TVADIVQSLPSFTATRSDDLILSSLHMANRELRRAKNHVQKWERVCAALRELNKHRDI
                     VRQITDFSRRVASEK"
BASE COUNT         1132 a         1082 c          830 g         1163 t
ORIGIN      
        1 acgactccgg acggcagacc gcgcatgttt ttcggttccg gctccaccaa tattctgccg
       61 ttatggaata ctgacattga ttcacggcct ggcttaacga taaccagacc gagttccggt
      121 accagaacag gtcgaagtaa tacccgcacg ttacctccag atgcgctgct ggaatgtgcg
      181 ggacggacgc ggtgggcgtt cggaataagg gagcctggcg gagattatcc agtgacgacg
      241 atcgaagctg agatctttct gaaactcgta accacgtctg cggtagcact gaatcagcca
      301 ttcggcctgt tcttcagtgc atgggtcatg ctggaaccag tcagatttga atgcatgaga
      361 acaccgcccg tgcctgctgg caaagacggc tgaattatca gaattgttaa gtttcttgta
      421 atgcaccact actatcctcg taatggtgcg acagacgcca gttgttcagg ctggctttac
      481 tacagcatta taatctagtc tcttttaatg ctgaagtaac tgaccgaatc cgcatgcgat
      541 tctttgctga tcagaataac atcagatggc aatggcatta cgataaactc accattccca
      601 agaactatta cttcataatt acctggtata gaaagcgcag caattaattc cttatcgttc
      661 ataacaaaaa tcccatgaac tataaacacc tccccttatg ggggccatcc ctcttatccc
      721 tgcgcgctac ttaagagcct cgattctagc cgtgaagaca tgccaatcaa cagcaataaa
      781 ccacctgaat aacagaatca gccagccaca tcggtacaac cagatgcatc acgctcaccg
      841 aaaataaaca acaaaaaacc gccgaagcga gttaagtggg gtgcgttgag gatgcctgac
      901 acatcagagg tggcgaggga tttctccccc gccaggtctc ttactcctca ggttcgtaag
      961 ctgtgaagac agcgacctcc gtctggccgg ttcggattcg tacctcgcag aggtctttcc
     1021 tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg actaacatcg
     1081 ccttttgctg cttcatagcc tgcttctcct tgcctttcgg cacgtaagag gctaacctac
     1141 gtttgtgaag catagattgg gcctcagatt aatgttaagc gtcttgcagg acgcgtaatg
     1201 ttaactgggg cttttctcta tctgcctttt ggtgttcatg cctgaggcag atagcctcaa
     1261 gcacccatta tgattttaac caaagtcttt gtatctataa agaggcacaa tattactcga
     1321 tatatcatca tgtgtgcgcc ttattccaaa cattttggaa acaaagcgtt ccctcactcc
     1381 cgacatatcc agtatctgct tccaagaaga tatatccagc aattcaactg cacaggaaag
     1441 taagcgaggt ttttcctcag ggattaactc atcccccatc tctcctttaa tcgtccctct
     1501 tttatttaga tggataaatc ccgttctcat ttgttgtggc gtaatcagcc ccaaaaccgt
     1561 agcacgataa atacacatgc gaagactgat tttccatcgt tctttgaaat taattagtgc
     1621 attccagtca agatgcgatc ctcgcatttt aggaaattca ttaacgaacg aaactctagg
     1681 tacaaggaat gcgctcgaaa aataatccgc atgatgttct gtaattttat cccctgttat
     1741 aatcccttca tgcaaaacaa gatgccccag ttcatgaccg agatctgagc gaaatcgaca
     1801 cacactcttt ttcgcggaat ttctaattat caatgggcgt ttactattaa tagtaaacgc
     1861 atcaatacga tcatccacgc cagatacatg agcaacaaca accccaatac tttccacgaa
     1921 attaaccatt gaagatatcg gtcccagccc caaaccccaa taccttctac aggcctcggc
     1981 aattctttca atatcttcat cggagttcaa aggtaatccg gacgtatcag gaaagttcaa
     2041 ttctggaaga actatttctg tttccaaaca ctttacaaac tgctccaaaa tttcagctcg
     2101 agccagaata ctattagtta aggactgcgt ccttgatctc ttgcttctga aatggcagtt
     2161 ctcgctgtct aatggtgatt tccttccagt gaagaaaaac gactctttaa catccagata
     2221 atctgcaaga gctgtcaaca tctgagatga agggggataa ccacgctcta acttgtttat
     2281 aaattgccga gtcaccccca aagcagtagc aagctcttcg cccgtaaccc ctttagcctg
     2341 acgagctaat tttaagcagt caccacgata attacctata gaatcaaata gttccatatt
     2401 tatcactatc tgcttttttc gcattcttaa cacgccgctg caaattcagc ggtggtgttt
     2461 ccacggcctc tggcaatacg tcagtattag ctgcttgtac tggagcatgg actttagaca
     2521 ccaattcgta tctactcact acattatttg atgaatcaaa acctatcagg gctaaaagcc
     2581 acgttggagg ttcaatatct tcatcttttt tatttgcaga tggtaatttt tcagccagaa
     2641 cacgccatgt aacatcatgt tcaaaattaa catcatcttc aaaaagagat aactgacgac
     2701 actcaacatc atttttacat aagcgatgtt tcttttttgg tgaatccagt ttatcggtca
     2761 caaactgtaa cggcaccctg ttcagggtaa acacaaaatt cagaggtgtt gaaagaatcg
     2821 ccactccttt gagtttttta tgatttttta caagaaagtt ttttatccat ccataggccc
     2881 tacatccatt aatccagtta tcatcatact catggcgatg gaaccataat tcgccaagta
     2941 cgttagcaaa ttgctgaaga agattgataa cagatttttc atccaaacaa ggattgattt
     3001 caaaacacgg ttggagattc tgttcagacg gcataaggta tccctctctc ttaaaactcc
     3061 gctcatcttt acgcaaaatt ttatttttgt aaacctaatc tttaggggat ttcacgaatt
     3121 cgtcaaccag tcgaacaaac tttaaccaca atacatattt atctacattg atatgtgtat
     3181 cgctaacact attgtgggca ttcagctagt attaccaagc cttcaggtgc gcgaagttgt
     3241 tccgcacagt gcagcagcgc atcagtcgct tccttaagcg tcacagtgtc gccatcaccc
     3301 agtcctgcaa tttttgcgtg cctgacaaac gccgcgcaaa ggtcgttaaa cgcccctgcc
     3361 cgcacatccg ccaggaaagc gttggcagca gccaaatcct ggtagtcaat cccgctcact
     3421 gtgccacctc ctgaaaattc ccctgataaa acgccagtgc gcgctgcata acttcgctct
     3481 tccggcactc gctacagatt atgttctgac gcctgtcgta gcggcgtatt tctccgtctg
     3541 gtaatgacca gataaggtcc ggatcatcca cagatgtttt cttcagattt gcccttgaga
     3601 gttttttgcg ggcgttttgc cagtccttac gcgcctgttc agacgggaat aacccgtaac
     3661 cagagttgta tacatcgcca ctggcaacca gctctctggc gagaacactc atcagatatc
     3721 ttgtcgcacc tgttttctct tccagttgcc gtaacgtctc gcggccgctc agacgtacaa
     3781 gttcaacaac ctgcccttta attttttccc gctcttctgg tgtaaatact tttgccatgg
     3841 gcgcctccgg caatcacttt tccgatgcaa cacggcggga aaaatcagta atctgtcgaa
     3901 caatatcccg atgcttgttc agctcccgca gcgcggcgca gacacgctcc cacttctgga
     3961 catgattttt cgcccgacgc agttcacggt ttgccatatg cagcgatgac aaaatcaaat
     4021 catcagatcg cgttgcagta aacgatggca gcgactgcac aatgtccgcc acagtttctg
     4081 ttttaatatc ttcctgtgtt gcagcctcct gtactggtaa cgcaacacat gcaggctgag
     4141 gaaaggcttt accatcagtt tccgctaccg atgctgcttt cggctctgct ggtaaattat
     4201 cgcccgg
//
LOCUS       DAZFYC010000232         4064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFYC010000232 DAZFYC010000000
VERSION     DAZFYC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.237"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..1408
                     /locus_tag="ACHKW5_005113"
     CDS             263..1408
                     /locus_tag="ACHKW5_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO7514290.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPFSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
     gene            1555..2373
                     /locus_tag="ACHKW5_005114"
     CDS             1555..2373
                     /locus_tag="ACHKW5_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO7514291.1"
                     /translation="MKRSLFIAFFIAMFVTPIALILFVFDYGYKKSPQEYSLSFIKQL
                     PNTVNLSSLTYNKEDDFLYATQNSPAQLLKIARSGDIIGRAPLPFISDAETIEHIQGN
                     IFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFEGLAWKAEGRMLFVAKE
                     RRPTGMFAYQLSPDLLSAKQVTIPEELNDIHVKDISGLDFNNESLMILSDESRKLLKF
                     NLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVTLPDESIYVASEPDILAKFVPNK"
     gene            2579..3454
                     /locus_tag="ACHKW5_005115"
     CDS             2579..3454
                     /locus_tag="ACHKW5_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO7514292.1"
                     /translation="MFSIRSQLPISASVSIPAEQSQSISTIPGGRKIETVPEKEGLLV
                     FLGTKSVGEYALNILGQNVSRVTTGKKPYDILFLHEATKQDFDKKKTEFTFPGANRSY
                     LQSSNTDVAAAAAISIAATEMKTILPKDLTPEKYNKIYLPGDGSAGLPLLKCGDEFLS
                     PTDIVNRLVEHNLHEVEDIRLTSCHSANITKNNDFSPEEIEKSTKTNSGWLASALFGQ
                     KRSLAEHVYAEFEGRGINVSVSGYHGTGVFYVPEHGKPTTHLRSTTVPATPEQTVRRS
                     DYRVSLGRTQPTDID"
     gene            complement(3694..3867)
                     /locus_tag="ACHKW5_005116"
                     /pseudo
     CDS             complement(3694..3867)
                     /locus_tag="ACHKW5_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1349 a          851 c          718 g         1146 t
ORIGIN      
        1 atcagcacaa taaaaatttt ttctgaatgc tattattaat gtaaataatt acaacctttt
       61 cgtaggcaca aaaactattg ctgatgaatc aatgaaaatc tattgttcgt cattagagga
      121 gtatttcctc taatttaaaa aaataactct ctatacattt aaaactctcg ctataaatag
      181 cttatatatt attgacacta tatcatgcta tatgatatct gttgtcgaat cattcataat
      241 aaatgagata ggggaatctc tcatgataaa cggacttaat aataattccg catctttagt
      301 tttagatgct gcaattagaa ttaattctga ttttaaaaag cagtggaatg atatgtcatg
      361 cgctgaaaaa ttactaaaag ttcttagttt tggtttatgg aatccaacgt atactcgtag
      421 tgaaaggcaa acatttcaag agttgttaac cgttttagaa cctgtatctc ccgcgcctaa
      481 tgaattgggc agaatatatg ctaattttgc agatggctca tccttaagaa tttccgtcac
      541 taacagcgaa cttgttgaag ccgagattcg cacaccagat aatgaaaaga ttcttatgct
      601 cctggagtca aacgaacaaa ataggttatt acaatcttta cccatcaatc tccacatgcc
      661 atacattcag gttcatcgtg ccttatctaa gatggacctg actgatcata agtcaatgca
      721 caatctactt agttttacgt caaaactatc agcaaccttg attcctcata atactcaaac
      781 agatccgttt tccgggccta caccattcag ctctatgttt atggatacat ttcgaggact
      841 cggcaatgca aagctttcac tcaatggtgt tgatatacct gtagatgcac aaaaattgct
      901 tcgcgatgca ctaggactta aagacacaca ttcatcactt gcccggaatg ttataaataa
      961 tggtatttct cgtcatcatg cagagcaaat agcaagggaa agcagcggca gcgataaaca
     1021 gaaagctgaa gttgtggagt ttttatgcca ccccgaagcg gcaacagcca tatgctcggc
     1081 tttctatcaa tctttcaatg tgccagcctt aatgttgaca catacaagga tctctcaagc
     1141 cagagaatac aatgtggaaa gatcgttaga tgtacctaac gcgtgcatta acataagtat
     1201 ctctcaatca ccagatggaa gcattcacgt tgccagccat accgggattc tgataatggc
     1261 gccagaagac cgccccaacg agttaggaat gttgacgaac aggacctcct atgaagtgcc
     1321 acaaggtgtg aaatgtgaaa tcgatgagat ggtaagaacg ctacaaccaa ggtatggcgc
     1381 atctgaaaca tacctgaaaa acatttaaaa ataaaaaaat ggacaccaca cactttattt
     1441 atcaaaacaa acaataacta atcacactca caattattaa taatttaacc tcacagcaat
     1501 catacaaaac aaagatattt atttcgcaaa ccatttatag cgaatggaga aggaatgaaa
     1561 agaagcttat ttattgcatt ttttattgca atgtttgtca caccaattgc attaatatta
     1621 tttgtatttg attatggtta caaaaaatca ccacaggaat actcactttc atttataaaa
     1681 caattaccaa atacggtgaa cttatcatct cttacgtata ataaagagga tgatttcctg
     1741 tatgccaccc agaatagccc tgcgcagcta cttaaaatag ccagaagtgg cgacatcatc
     1801 ggccgagccc cgctgccttt tatctctgat gccgaaacca ttgagcacat acaagggaac
     1861 atttttgctg cagtagacga aaagacttct gaactattct tttttacagt tacaaaagat
     1921 atgcatatct ccttgcgaaa taaaattcag ttagaaaaat tcaataaaaa aaatagaggg
     1981 tttgaaggac tggcatggaa agcagaaggc cggatgctgt ttgtcgctaa agaacgacgc
     2041 cctacaggca tgtttgccta tcagctttct ccagatctgc tgagtgcaaa acaagtaacg
     2101 attccagagg aactaaatga tatacatgtt aaagatatat ccggcctgga ctttaataat
     2161 gaaagtctga tgattctgtc cgatgaatcg cgtaagttac tcaagtttaa cctgacagaa
     2221 atgtctttcg ttgagatgat ggacctgaca aaaggaaatc attctctgac cagtgatttg
     2281 ctccaacctg aaggcattgt aactctgcct gatgaaagca tttatgtcgc tagtgaacct
     2341 gatatcctcg ctaaatttgt ccctaataaa taagtctgtc actagcctta gtattgagcg
     2401 accaaatctg gcaatctcat gaaactcgca aaatacgaat ctaatattaa taaaaatttt
     2461 actctgaaat aaacagatta atagctgaaa aggatatcag ctattatcaa ctaaaatatt
     2521 actcactcca ttagaattat catgaactca catttaaaca aactaagaag gaggttgtat
     2581 gtttagtata agatcccagc tacctataag cgccagcgta tcaattccgg cagaacaatc
     2641 tcaatccatc tccacaattc ctggagggag aaaaatagag acagtgccag agaaagaagg
     2701 attgcttgtg tttttaggaa caaaatccgt aggtgaatat gctcttaata ttcttggtca
     2761 aaatgtttca agagtcacaa cagggaaaaa accttatgat atattattcc tgcacgaagc
     2821 tacaaaacaa gattttgata aaaagaaaac agagtttacc tttcctggtg caaacagaag
     2881 ttatctacaa tcaagtaaca ccgatgttgc tgccgcagca gctataagta ttgcggcaac
     2941 agagatgaaa actatcctac caaaggattt aacaccagaa aaatacaata aaatttatct
     3001 gccaggggat ggttctgctg gcctacctct tcttaagtgt ggagatgaat ttttatcacc
     3061 aactgatatc gtcaatcgtc tggtagaaca taatcttcat gaagtagaag acatcagatt
     3121 aacatcctgt cactcagcca acataacaaa aaacaatgat ttctctcctg aagaaataga
     3181 aaaatcaaca aaaaccaaca gcggctggtt ggctagtgca ttatttggtc aaaagcgttc
     3241 attagcagaa cacgtctatg ccgagtttga aggccgtgga attaacgttt ctgtatccgg
     3301 ttatcatggc actggtgttt tttatgttcc agagcatggc aaaccaacaa cgcatctacg
     3361 ctccacaaca gtgcctgcaa cacctgaaca gacggtaaga agaagtgact acagagtctc
     3421 ccttggcaga actcaaccca cagatattga ctaattgagt ctgcatatag gtttgtgtca
     3481 ttacctgatg cgctacgctt atcagaccta tattagatcc tgcaatatgt ttaatttgta
     3541 ggcagaatag agcgttcaca ccacatcagg tataaacaaa gtacagcttg ccaaaaacct
     3601 gaataacccc gaatttacgg ggttattccg cttaaacatg cccataacat cacttctgat
     3661 ttgtattgct tacgacggat acaatttctc tgctcagcta cgaatatcat catactcgcg
     3721 cgttttatcg aactcgtgtt tcgcaaacgg acatagcggg ataatcttcc gcttctcccg
     3781 gcgcattttt tccaccacct tcgccaccag ccgtttacca atcccctgcc ctttcaggct
     3841 ttcatcaaca tcggtatgtt cgataatggt cagttatcag ggataaccct gtgagtacaa
     3901 cgcaatctgt tgctactggg tagactggtc tgctgatcac actcacagat ttatttcaca
     3961 tatatgaact catgtatttg atctctctct tatatattta ataccaatca gacagtctta
     4021 tcccaccagt gatacctgtc cctgcccaca aggcacgggc tgcc
//
LOCUS       DAZFYC010000233         4060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFYC010000233 DAZFYC010000000
VERSION     DAZFYC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.238"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..891
                     /locus_tag="ACHKW5_005117"
     CDS             49..891
                     /locus_tag="ACHKW5_005117"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7514293.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKILSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            894..1982
                     /gene="capU"
                     /locus_tag="ACHKW5_005118"
     CDS             894..1982
                     /gene="capU"
                     /locus_tag="ACHKW5_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFO7514294.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARLAKQDIEERFDINKTALKILTLAKQK"
     gene            1987..2937
                     /gene="virK"
                     /locus_tag="ACHKW5_005119"
     CDS             1987..2937
                     /gene="virK"
                     /locus_tag="ACHKW5_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFO7514295.1"
                     /translation="MFSISNLSFIGFLKRIICSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEYQNLN"
     gene            3002..3946
                     /gene="lpxM"
                     /locus_tag="ACHKW5_005120"
                     /gene_synonym="msbB"
     CDS             3002..3946
                     /gene="lpxM"
                     /locus_tag="ACHKW5_005120"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7514296.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT         1191 a          760 c          913 g         1196 t
ORIGIN      
        1 tattaggctt tccttttatc tctacttact gggtgccaga aactttaaat tttaaacgga
       61 gggggcattt tgtttaaagc aaatcatgtt ccggtattaa tgtatcatca tgtcagccat
      121 tgcccaggac tggttacact ttctcccgag acattccgga aacaaatgaa atggttggct
      181 gaaaacaact ggaaaatact gtcatcagat gaactggagt ttttttatcg gggcggaaaa
      241 cttccccgta aaagcgttat gttgacattt gatgatggat accttgacaa ctggtttcag
      301 gtatatcctt tattaaacga gtttaatctc aaggcacata tttttttaat taccagtttt
      361 attggaaatg gtccggttcg tcactctcct ggaaaggaat attctcatcg cgattgcgaa
      421 catcaaatag caaccgggaa tgctgataat gttatgcttc gctggtctga agtgaacgaa
      481 atgttgcaat ctggtctggt cgaatttcat gttcatacac atactcatac acgttgggat
      541 aaaaagttca cttcacgaga agaacagtgt aaacatcttc ggcaggactt attgagcggg
      601 agagaatatt taaaaaaaat gacaggaaaa tgcagtaaac atttgtgttg gccggaaggt
      661 tattataaca aggattatat tcagatcgct gaagaacttg gattccatta tttatataca
      721 acagaaagaa gaatgaatgc tcctgctaaa ggaactacac ggataggaag aataagcacc
      781 aaggaacggg aaaattgcgt atggctgaaa agacgacttt tttattatac aacaccgttt
      841 ttttcatcgt tgctggccct tcataagggg ccaagattac cggatgacta ataatgaata
      901 tactatttac ggaatcatca ccgaatatag gtggtcagga attacaggct gttgctcaaa
      961 tgaaggccct gaagaaaatg gggcattcag ttctgcttgt ctgcagggag aacagcaaaa
     1021 ttgcttttga agccagtaag tttggaattg atatcacatt cgcgttattt cgaaacagtc
     1081 ttcacattcc tactgtatgg agattactcg gaatagttca tagttttcag cccgatgcaa
     1141 tcgtttgtca cagtggacat gatagtaata ttgttggttt ggtacggttt tttacctgga
     1201 aacatccatt cagaattatc aggcaaaaga catatttgac acgaaaaaca aaattttttt
     1261 caataaatca tttttgcgat gaggtgattg ttcccggaac aaatatgaag acacatctgg
     1321 agcaggaagg atgtcgaact cgggttactg ttgtgcctcc tggctttgat ttccaggaat
     1381 tatacgttga ttcccgaaac agtttgcctc cgagtgttct ttcctggctg gcgtcccgaa
     1441 gaggatgccc cgtaattgct caggtaggaa tgttgcgccc ggaaaaaggg cacgaattta
     1501 tgttgaattt actgttccat ttaaaaatga atggacgaca gttctgttgg ttgattgtgg
     1561 ggtctggttc acctgaactg cgggaacatt tacagtatca gattgacagt atgggcatgc
     1621 atgatgatgt ttttattgct gacaatgttt ttcctgccgc ccccgtatat cgggttgcca
     1681 gtctggtggt tctgccttca gaaaacgaat cttttggtat ggtgctggca gaagcatcgg
     1741 cattttctgt gcctgtagtg gccactcaga ttggtggaat ccctgaggtt attcagaaca
     1801 accagaccgg gacattgtta ccagcaggta ataagcacgc atggatgtgc gccctgaatg
     1861 atttttttaa tgaccctggg cgtttttatc agatggctcg cctggcaaaa caggatatag
     1921 aagagcggtt tgatattaat aaaactgcgt taaaaatact cacattagcg aagcaaaagt
     1981 aacaatatgt tttctataag taacttatca tttatcggtt tccttaaaag gattatttgt
     2041 tcctcagatt cactaccggg gaagtgggaa cacagaaaat ttcggttcat gtacattttg
     2101 cgatgttcta taaatccggt tgtcagtatt cgatattatt atgaactgcg ttccttgccg
     2161 tgcattgagg atattctggc aatacacccc acgttgccag caagaattca tcgcccttat
     2221 ttgcataaag gggggcgcgc ctggacccgt ggacaatata ttcttgagca ttatcgcttt
     2281 gtgcaaaatt tgccagaaaa atattctaaa tttttgtttc cacaaaaatc ggtatcatta
     2341 gtccaattca tcgggaaaga tggagagaat tttgatatcc agtgttctcc cagtggtttt
     2401 gaccgggaag gagagttgat gttgtcattg ttttacaata aaacagttat tgcccggtta
     2461 acattttcag tcattctgac tcaaaatgga cacattgctt ttattggcgg attacaggga
     2521 gcccctaaaa ataccggacc tgatgttatt cggtgcgcaa cccgggcctg ttatgggatt
     2581 ttcccaaaac ggattatttt tgaagcattt tgtgcgttga tgaaagcttg caatatctct
     2641 gagtgtctgg cagtcagtga gcacagtcat gttttccggc aattgagata ctggtatcag
     2701 aaacgcaaaa cctttgttgc ggtctatagt gatttctggg agtctgtagc aggaaaaacc
     2761 tgtggtgact ggtacagatt accaacccag gtgattcgaa agccgctaag cgatatagcc
     2821 agtaaaaaac gctctgagta ccgaaaacga tacgctttgc tggattatat ccatgaaact
     2881 actatccgct ctttggatgc atatcctgta cactcagaat atcagaattt aaattaaggt
     2941 ttgatgtatt ctctgaataa aacagtaatg ataattttgg tagaggcatt cgcactaaat
     3001 aatgaaaaaa tataaatctg agtttattcc tgaatttaag aaaaattacc tttcccctgt
     3061 ttactggtct acatggttcc ttttgggaat gattgcaggt atttcaatgt ttcccccttc
     3121 attcagagat cctgtcttgg caaaaatagg gcgttgggcg ggcagattga gcaaaaaagc
     3181 tcgtcgcagg gcgacgatta atttatcgct ttgtttccca gaaaagagtg atacagaacg
     3241 ggaaataatt gtcgacaaaa tgtttgccac agcattgcaa tccatagtga tgatggcaga
     3301 actggcaatt cgtggtccgg aaaagttcca gaaacgagtg ttctggaaag gcctcgaaat
     3361 tcttgaggaa attcgacaca ataatagaaa tgtgattttt ctggttcccc atggctggag
     3421 cgtggatatt cctgcaatgt tgctggcagc tcagggaaaa aaaatggccg ccatgtttca
     3481 tcagcaacga aatccagtga ttgattatgt ctggaattca gtacggcgta aattcggggg
     3541 gcgcttacat gcccgggagg atgggataaa accatttatt cagtcagtac gccagggata
     3601 ctggggatat tacctcccag atcaggatca tggtcctgaa tacagtgaat ttgctgattt
     3661 ttttgcgacc tataaagcga cattaccaat cattggacgt ctgatgaaca tcagtcaggc
     3721 tatgattata ccacttttcc cggtttatga tgaaaaaaaa catctactga ctattgaaat
     3781 tcggccacca atggatgctt gcattgccag cgcggacaat aaaacgattg cccgacaaat
     3841 gaacaaaaca gtggaaattt tggtggggcc acatccggaa cagtatgtct gggttttaaa
     3901 attgttaaaa acacgcaaat caaacgaagc ggacccgtac ccttgaaatt ataaagtaca
     3961 agtatttcca ctagttgttt gtaagcgtac agcctgaacc gtctggtcag aatctgacga
     4021 attagacaaa gtggtgccca ccaaataagt agtgggaacc
//
LOCUS       DAZFYC010000234         4025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFYC010000234 DAZFYC010000000
VERSION     DAZFYC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.239"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..377
                     /locus_tag="ACHKW5_005121"
                     /pseudo
     CDS             1..377
                     /locus_tag="ACHKW5_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(564..1751)
                     /locus_tag="ACHKW5_005122"
                     /pseudo
     CDS             complement(564..1751)
                     /locus_tag="ACHKW5_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element IS91 family transposase"
     gene            complement(1751..2116)
                     /locus_tag="ACHKW5_005123"
     CDS             complement(1751..2116)
                     /locus_tag="ACHKW5_005123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514297.1"
                     /translation="MARSAKPRKRKPAQQRSKLPRYVVKLHPDDFFDEEDAEVLRFDN
                     FDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECSTVHWPDGVTPPV"
     gene            2644..3153
                     /gene="def"
                     /locus_tag="ACHKW5_005124"
     CDS             2644..3153
                     /gene="def"
                     /locus_tag="ACHKW5_005124"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO7514298.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIRRIVDDMFETMYAEKG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            3168..3269
                     /gene="fmt"
                     /locus_tag="ACHKW5_005125"
                     /pseudo
     CDS             3168..3269
                     /gene="fmt"
                     /locus_tag="ACHKW5_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="modifies the free amino group of the aminoacyl
                     moiety of methionyl-tRNA(fMet) which is important in
                     translation initiation; inactivation of this gene in
                     Escherichia coli severely impairs growth; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
     gene            3466..3657
                     /locus_tag="ACHKW5_005126"
     CDS             3466..3657
                     /locus_tag="ACHKW5_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="HFO7514299.1"
                     /translation="MTLSFFITVIPGHDKPQPPGCLLDEILKRVIPAIYNVAIDDAIK
                     SVRNVGDRLEEELDMRKVV"
     gene            3654..4016
                     /locus_tag="ACHKW5_005127"
     CDS             3654..4016
                     /locus_tag="ACHKW5_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFO7514300.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVI
                     YKKHQLRKIDMRPARILPGIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIEN
                     WIKVNGGWKMQFISYEKI"
BASE COUNT         1098 a          970 c          950 g         1007 t
ORIGIN      
        1 gaacaggtcg ttccggcctc tgaacttgct gctgccatga agcagattaa agaactccag
       61 cgcctgctcg gcaaaaaaac gatggaaaat gaactcctta aagaagccgt tgaatatggg
      121 cgtgcaaaaa agtggatagc gcacgcgacc ttattgcccg gggatgggga gtaagcttag
      181 tcagccgttg tctccgggtg tcgcgtgcgc agttgcacgt cattctcaga cgaaccgatg
      241 actggaaaga tggtcgccgc agccgtcact cagatgatac ggatgtgctt ctccgtatac
      301 accatgttat cggagagctg cccacatatg gttatcgtcg ggtatgggcg ctgcttcgca
      361 gacaggccga acttgatcga taggaattta aatccccaaa agactaaaaa agcaccacaa
      421 aacggatgtt tcttcaacac cccttgtgca ccatatgagc ggaaacgact attaaacagg
      481 atgacactga ttgatacagg gtatgaatgc cggttttttg ctccgtttcc ggtaaaatgg
      541 acgcaacttc ccctctgcgg ctctcagccg cacacctgct gtcgtgccag tcgctcatgc
      601 atcaggacca gctctgccag tcggtaaccc cgcttcagcc ccgtaaaacg catctgactt
      661 ccgcacagaa tgcacttcag cgggtcaacc ttcagtaacc tctgatacat ccctctccac
      721 ctgatttgca tcgccgtttt tctcaccgtc tccgttatga cgtacaccac ttcttccagt
      781 aagcggcgct tcaccggact caggaaaccg tagtacctca ccatacggaa ccccttatct
      841 gctacatgcc aggagaacct ttccatgaac tcatctccac tcatcaacag atattcttcc
      901 cgttttgtac ggtgactgtt gtaacgcaga ccgatttcat cctgaccggc ataatgctcc
      961 agccgactca ccggcaccgg tggctttttc aggtaagagc cgaagtacac cgccacatgt
     1021 gtggcgttat ccatcaccct ggatatgttg acattccagc cgcggcggta atgcgtgtcc
     1081 aggaagcgat tccagtcccg tttgctgttt ccttccactg ccagttcatc cggtattacc
     1141 agctccgggt atttccggga cagtagccgc gttatccggt aacgccacat gctcatcacc
     1201 ttacgggcgt aaaaatgaag atttttccag gtgtggcccg acgtcacacc accggcagtt
     1261 gtcgataaat ggatatgcgg atgctactgc tggtcacgcc cccatgtgtg gatcaccgtg
     1321 aatatccccg gctcaacatc tgcctgatgg cagatttcca gtatcacatc agctgcaatg
     1381 cggctcatct ctgccagtaa ccaccggttg tggaacacca gggaccagta ctggcaggga
     1441 agtgtgaaca caatatgctg ccacgggcag tcggggacca ggctcagcag atactgtatc
     1501 cactgtgcgc cagccttcac cccgcagtgc ggacaggagc ggcttttaca ccggaagcag
     1561 acctttttgg tgtggcaaca gtccggtgac gaacagcacc attgtgtgta gcccatcagc
     1621 gtggtcccgc atgccatgat ttttgtcact gactcagtca ccaccggacg cactgaacct
     1681 tccggctgtt tctccagcca gttaagccag cggttaccct gctgaaaaat atcggcaaaa
     1741 cggggaagca tcagacgggt ggggtaacgc catccggcca gtgaactgtg ctgcattccg
     1801 ggcagtacat accaccggca ctgataccgg caagaatggt tgcaaactcc cgctccgtac
     1861 agcgggctat ttccggatac ccttcgtcat cgacacgaac aaaccagaag accagctttt
     1921 tgtttccagc atccagaaag aacggaatac ccaggtcagc gcagcactca acggcatcgt
     1981 caaaattatc aaagcgcaga acttcagcgt cttcttcgtc aaaaaaatca tccggatgaa
     2041 gtttcacaac atagcgggga agtttgcttc tttgttgtgc gggtttgcgt ttacggggtt
     2101 tagctgaacg ggccatataa ctacctgaaa gacaatgaca ttatcatttt ttataacggt
     2161 aattccaggc catgacaagc cgcagccgcc aggctgccta ctcgataaat taaccttcat
     2221 ttgatgctgg attgaacata tcaaaatcag gcaattacac aaatctatgt acaggctcgg
     2281 aaattgaaaa ccagacgata ccgctgtcca cgccttatga cacgtgagcc tttctgcttt
     2341 tcccaccact ccggtggttt ctctctctgc atggtcgatt tttataccgt gacaggttgt
     2401 tttttatact tgcaccgatt cccgggcaca cttagcctgt gtcaggccgg caattagtag
     2461 ccggtttaaa actgggataa cggaaaaatc tggggttccg gcgtagaacc ccatccttat
     2521 ctccctgcca taagcagcct tagaaatctt tgcgattggt cagtgatgct gtcaatcaga
     2581 gggggatttg tctagaatag aagaaataat ctttctaact cctgaacaca tctctggaga
     2641 cttatgtcag ttttgcaagt gttacatatt ccggacgagc ggcttcgcaa agttgctaaa
     2701 ccggtagaag aagtgaatgc agaaattcgg cgtatcgtcg atgatatgtt cgagacgatg
     2761 tacgcagaaa aaggtattgg cctggcggca acccaggtcg atatccatca acgtatcatt
     2821 gttattgatg tttcggaaaa ccgtgacgaa cggctggtgt taatcaatcc ggagctttta
     2881 gaaaaaagcg gcgaaacagg cattgaagaa ggatgcctgt cgatccctga acaacgagct
     2941 ttagtgccac gcgcagagaa agttaaaatt cgcgccctgg accgcgacgg taaaccattt
     3001 gaactggaag cagagggtct gttagccatc tgtattcagc atgagatgga tcacctggtc
     3061 ggcaaactgt ttatggatta tctgtcaccg ctgaaacaac aacgtattcg tcagaaagtt
     3121 gaaaaactgg atcgtctgaa agcccgggct taaggataag aactaacgtg tcagaatcac
     3181 tacgtattat ttttgcgggt acacctgact ttgcagcgcg tcatctcgac gcgctgttgt
     3241 cttctggtca taacgtcgtt ggcgtgttca tcgataggaa ttaaaatccc caaaagacta
     3301 aaaaagcacc acgaaaagga tgtttcttca acaccccttg tgcaccatat gagcggaaac
     3361 gactattaaa caggatgaca ctgattgata cagggtatga atggcggttt tttgctccgt
     3421 ttctggtaat ttagctgaac gggccatata actacctgaa agacaatgac attatcattt
     3481 tttataacgg taattccagg ccatgacaag ccgcagccgc caggctgcct actcgatgag
     3541 attcttaaaa gagtaatccc agccatatat aatgtggcta ttgatgatgc tataaaatca
     3601 gtaaggaatg ttggggatag attagaggag gaattagata tgaggaaggt agtgtgacta
     3661 attttaaatc agacttaaat ctaataagaa aagaatggta taatgcattt tattcaggta
     3721 atactgaaat gctagattac ttggaagctg attggtttat ttcaacaaat ggtcaaaaag
     3781 ttatctataa aaaacatcag ttacgaaaga ttgatatgcg tccagcgagg attttacctg
     3841 gcatagaaag atatgaatat gatgtcgtaa tgcgtgagtt taaaggaatt gcttgtgttt
     3901 cagggaaagc tgaaataaag caaaatgatg gaaatatatt aatggcattt attgaaaatt
     3961 ggataaaggt taatggtgga tggaaaatgc agtttatatc gtatgaaaaa atctaagggg
     4021 aagcc
//
LOCUS       DAZFYC010000235         4016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFYC010000235 DAZFYC010000000
VERSION     DAZFYC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.240"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1326)
                     /locus_tag="ACHKW5_005128"
     CDS             complement(<1..1326)
                     /locus_tag="ACHKW5_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspK/GogB family type III secretion system
                     effector"
                     /protein_id="HFO7514301.1"
                     /translation="MLSTSQLKLSGTACHHITETPASFKSEITSRQLEDARESGCLHI
                     KNCDMEFLPHLPEEITAITIENCNKLTGLTGLPVNIKSLSVINCENLKVSELPPTVRE
                     LHIELNNSSFVHTVPEGVEHLKLGHCQVSGVPDSVRSLELTGRAAETLTGVPSGLCSL
                     SIRDYNPEYQVRIDNFISPSLQTLSLTGCSNIVLPEKLPKGMVSATIYTEQKTTWNIV
                     AEGFPEEMDLDLQNVQLSPDVIKEKNITFQGYAMEAALHFRLGDIVYGLSEPRGKVVN
                     TIKLVNDFSKKDILIQNTLTSAVWDYKSPRKYKKDALIKRALSESDRGINFKQYLKNH
                     SQYNVTMADLSVCNRDKLWAKTSKAGLEFQLLVRNKMVHFCADGLVDTLKNIANKGDA
                     YGQSITASELRWIYRNQDNEQIMKNIKFYLRGKEVPAEKILGMPEWKDYH"
     gene            complement(1618..2256)
                     /locus_tag="ACHKW5_005129"
     CDS             complement(1618..2256)
                     /locus_tag="ACHKW5_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7514302.1"
                     /translation="MPVVLNISDGRALPESELEALRNIARSNQSDTLIVGSRSLRLHH
                     ISFMDAFSVEPIQGSLLDRLGGRCRRLAESLERQLNHGNTFLQAFSLYMEQVRLTPFW
                     LEGGRNAVQTRINSHAFTVNPGDFPCCEQHLSCPITLCIPKTGVFVKNALHSKVCSLY
                     DKDALSEAIRHNVFHPLSREAFSPEMIVGREECYFDLTDQRFCIISGQDVRF"
     gene            2454..3038
                     /locus_tag="ACHKW5_005130"
     CDS             2454..3038
                     /locus_tag="ACHKW5_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFO7514303.1"
                     /translation="MPANISGTPFNSFGISFIQKQSCWRKSDDILRCSMGQRTIKLST
                     NTLNNRILTSVARQSTKDINAWKRDEQTVYPSRVINQGIDKYCAENSRNISSEVRQRV
                     FKFIEKDYSLKLNIIAAQSSINHLIIGNGRFGDKINMLCKGVSREVKNQTMDVIANQL
                     ADQFFQKHISPDVDIKQLRDDIPRYIMAASVISA"
     gene            complement(3284..4015)
                     /gene="espF"
                     /locus_tag="ACHKW5_005131"
     CDS             complement(3284..4015)
                     /gene="espF"
                     /locus_tag="ACHKW5_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFO7514304.1"
                     /translation="MSQAVSTLGRHITSAASRVSSAGLSGFSVSPQAVRLNPIRAQSP
                     FSPGTSNINARTTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKDHLAA
                     YEKSKSLEASGLKPARPAPPPPTSGQVSGSSRSLPPIAQALKDHLAAYEKSKSLDTSS
                     LKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLAAYEKSKSLDTSGLKPSRPAPPPP
                     TSGQASEASRPLPPIAQALKDKNRY"
BASE COUNT         1222 a          930 c          779 g         1085 t
ORIGIN      
        1 atgataatct ttccattctg gcatgcctaa aattttctct gccggaactt ctttcccacg
       61 tagataaaac tttatatttt tcattatctg ctcattatcc tgattacgat aaatccatcg
      121 caactcactg gctgtaatac tttgcccata agcatccccc ttattggcta tattttttag
      181 agtatcgaca agcccatcgg cacaaaaatg caccattttg ttccttacta ataactgaaa
      241 ctccaggcca gccttactgg tttttgccca taacttgtca cgattacaca cagaaagatc
      301 ggccatagtg acattatatt gactatggtt cttcaaatac tgcttaaagt taattcctct
      361 atcagactca cttaatgctc tcttgataag agcgtctttc ttatatttcc gaggagattt
      421 ataatcccat acagcacttg ttaacgtatt ctgtattaaa atatcttttt tagaaaaatc
      481 attaaccagt tttattgtat taacaacttt tcctctgggt tccgaaagcc catacacaat
      541 atcccctaga cgaaaatgta aagccgcctc catggcatag ccctgaaaag taatattttt
      601 ttctttaatc acatctggag aaagctgtac attctgtaaa tcaagatcca tctcttcggg
      661 aaatccttca gcaacgatgt tccatgtcgt tttctgctct gtataaatgg ttgctgacac
      721 catccctttt gggagttttt ccggcaacac gatattactg caacccgtca gagacaacgt
      781 ctgcagtgaa ggggatataa aattatcgat tctgacctga tactccgggt tataatcccg
      841 gatactcaga gaacacaacc ctgatggtac gccggtaagc gtctccgcag ccctacccgt
      901 caactcaaga gaccggacac tatcaggcac acccgatacc tgacagtgac caagtttcaa
      961 atgctcaacg ccctctggaa cagtatgtac aaaagacgaa ttattcaatt caatatgtag
     1021 ctctcttact gtcggcggca gttcactgac tttcagattc tcacaattaa taacggacag
     1081 gctttttata ttgaccggta gccctgtaag accggtcagc ttattacagt tctctattgt
     1141 tatcgctgtg atttcttccg gtaaatgagg caagaactcc atgtcacaat tttttatatg
     1201 cagacaacca ctttcccgtg catcttccag ttgccgggac gtaatttcac ttttaaagct
     1261 tgcaggtgtt tcagtaatat gatgacaggc agtccctgac aattttaatt gtgatgtgga
     1321 aagcatcaat aaattccatt ttcagaaaat aaatcactat aaggtaagcc ccatccgagg
     1381 agcttacctg acaaatttca tacagagagc ctgtatccag ggtttcccga tggcgaaaag
     1441 gcatcattac agacgctatt tcacgctgga aaagcaagta cagttactac atgttcattc
     1501 cagaataaac ccagcaatct gacccggatt ctaacaacca gcaaaataat gttgtcaatc
     1561 gaagacgaat aaaaaagcag cagtcagtac aactcagtct gtattaatat cccccactca
     1621 aaaacgtaca tcctgcccgg aaatgatgca gaaacgctga tcagttagat caaaataaca
     1681 ctcctctctg ccaacgatca tttctggaga aaacgcttca cgactgaggg gatgaaaaac
     1741 attgtgtcgg atagcctcag acaaggcatc cttatcgtat aaagaacaga cttttgaatg
     1801 cagtgcattt ttaacaaaaa cgcctgtttt tggtatacat aatgttatcg ggcaactcag
     1861 atgttgctca cagcaaggaa aatctccagg attgacggta aatgcatgag aattaattct
     1921 ggtctgcact gcatttctgc ccccttccaa ccagaatggg gttaaacgaa cctgctccat
     1981 atataaactg aacgcctgca ggaaagtatt gccatgatta agctgtctct ccagactctc
     2041 tgcaaggcga cgacacctcc ctccaagacg gtcaagaaga ctgccctgaa tcggctccac
     2101 actaaaagca tccataaatg aaatatggtg cagcctcaga ctacgacttc ccacaatcag
     2161 agtatcactc tgattgcttc ttgctatatt acgcagagct tccagttcac tttcaggcag
     2221 cgcccttcca tctgagatat ttaatacgac gggcatatta tctccctctg taatgtattt
     2281 agcttatcca ggccaacaac aaataaacat ccacacataa aataaaacag acaaaacatt
     2341 ttggcaacat accatcaatt attgacttat cattatttac accaaagcgt tgcgatacta
     2401 aaataattat aaacttgcta cattgtaaat atagacaaat aaaaggaatt actatgccag
     2461 caaatatatc aggaacaccc ttcaactcat ttggtataag ctttatccag aaacagtcct
     2521 gctggaggaa atctgatgat atcctgcgat gttcaatggg tcagaggact atcaagttat
     2581 ccactaatac gctgaacaac cgaattttga cctccgtcgc ccgacaaagc accaaagata
     2641 ttaatgcctg gaaaagagat gaacagacag tttacccatc tcgcgtgatc aaccagggaa
     2701 ttgataaata ttgtgctgaa aatagcagaa acatttccag tgaagtacgg caaagagttt
     2761 ttaaattcat tgaaaaggat tacagtctca agttgaatat catagcagcg caaagctcta
     2821 tcaaccatct cataatagga aatggccgtt tcggtgataa aataaatatg ctttgcaagg
     2881 gagtgagtag agaagtaaag aaccagacaa tggatgtaat agccaatcaa ctcgcagacc
     2941 aattcttcca aaagcatatt agtccagatg ttgacatcaa acagctacga gatgacatcc
     3001 ccagatatat tatggctgcc agcgtcatat cggcataaat agagataccc ttaacgctaa
     3061 ccagaaaaaa aatacctata agagcctgta catagagttg tgtcgttgcc tgatgttgat
     3121 atgtcaaatc caacatcaaa tagcggtaaa tttatggata aaaaattaca ggctctggtt
     3181 aacgaaacac ccccctcttg ctctatcccc ctctttcatc atttattaca atatttattt
     3241 caataaaaac cgatattacc aatcacattt atcacaatgc aacttaatat cggtttttat
     3301 cttttaatgc ctgagcaata ggcggtaatg gccgggatgc ctcggatgcc tgtccacttg
     3361 tcggtggcgg cggtgccggg cggcttggct taagacctga agtatcaaga cttttcgatt
     3421 tttcataggc agccaaatga tcttttagcg cctgagcaat gggcggtaaa gaccgggatg
     3481 acccagatac caacccgctt gtcggcggcg gcggtgccgg gcggcttggc ttaaggcttg
     3541 aagtatcaag acttttcgac ttttcatagg cagctaaatg atcttttaat gcctgagcaa
     3601 tgggcggtaa agaccgggat gatccagata cctgcccact tgtcggcggc ggtggagccg
     3661 ggcgggccgg cttaaggcct gaagcctcaa ggcttttcga tttttcatag gcagccaaat
     3721 gatcttttaa tgcctgcgca atgggcggta aagggcggga tgccccggaa gcctgtccac
     3781 ttgtcggcgg cggtggggcc ggacgagagg gagtaaatga agtcgccggg ctactcacat
     3841 taaaagtcgt tctcgcattg atattcgacg taccgggaga aaaaggagat tgagccctaa
     3901 tcggattcag acgaacagcc tgaggggaca ctgaaaagcc agataacccc gcagagctta
     3961 cccggcttgc cgcactagta atatgccgtc ctagtgtaga aacagcttga ctaatt
//
LOCUS       DAZFYC010000236         4010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFYC010000236 DAZFYC010000000
VERSION     DAZFYC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.241"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHKW5_005132"
     CDS             <1..759
                     /locus_tag="ACHKW5_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7514305.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACHKW5_005133"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACHKW5_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO7514306.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNISFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACHKW5_005134"
     CDS             2581..3900
                     /locus_tag="ACHKW5_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO7514307.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISTPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          950 a         1023 c         1006 g         1031 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggttgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggtgtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatct ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta catagtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tttctcggct cgactggcca ccggtttcct cggttaacat tagtaccccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gccgaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta tttctatacg
     3301 gagtcaccgg gttcactgct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccatgc ttactttgta acaagccggt gctttaccca ccggcgtgtt acaccttgtg
     3961 aaatatccca ctagtaatca tgcttacata agtcaaatta accacgcaat
//
LOCUS       DAZFYC010000237         3996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFYC010000237 DAZFYC010000000
VERSION     DAZFYC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.242"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            283..399
                     /locus_tag="ACHKW5_005135"
                     /pseudo
     CDS             283..399
                     /locus_tag="ACHKW5_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(504..580)
                     /locus_tag="ACHKW5_005136"
     tRNA            complement(504..580)
                     /locus_tag="ACHKW5_005136"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(544..546),aa:Arg,seq:tct)
     gene            complement(594..670)
                     /locus_tag="ACHKW5_005137"
     tRNA            complement(594..670)
                     /locus_tag="ACHKW5_005137"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Arg,seq:tcg)
     gene            complement(678..753)
                     /locus_tag="ACHKW5_005138"
     tRNA            complement(678..753)
                     /locus_tag="ACHKW5_005138"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(718..720),aa:Ile,seq:cat)
     gene            complement(782..1495)
                     /locus_tag="ACHKW5_005139"
     CDS             complement(782..1495)
                     /locus_tag="ACHKW5_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514308.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1633..1830)
                     /locus_tag="ACHKW5_005140"
     CDS             complement(1633..1830)
                     /locus_tag="ACHKW5_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7514309.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(2117..2935)
                     /locus_tag="ACHKW5_005141"
     CDS             complement(2117..2935)
                     /locus_tag="ACHKW5_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO7514310.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(3087..3458)
                     /locus_tag="ACHKW5_005142"
     CDS             complement(3087..3458)
                     /locus_tag="ACHKW5_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7514311.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(3448..3819)
                     /locus_tag="ACHKW5_005143"
     CDS             complement(3448..3819)
                     /locus_tag="ACHKW5_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7514312.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(3832..>3996)
                     /locus_tag="ACHKW5_005144"
     CDS             complement(3832..>3996)
                     /locus_tag="ACHKW5_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514313.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT         1258 a          993 c          769 g          976 t
ORIGIN      
        1 tgtttaaatg ccatgaatcc tcccggccgg gataatattg tgagtaaaat gaggagcggg
       61 ctgaagtccg gaagttacag gacaatggca gaagagagac aacagcccgc aatacgaaaa
      121 aggccgcgct attgcgcaga gtgattactg tcggatatta ttcgccagct gaaatattac
      181 ttcacgtttt gttgtttatt ccttgccgcc cgcgtctccc tgcgcgggct ttttttgtcc
      241 ataagaaagc ccctccggag aggggctgga gagtggcgct atgtgccatt gcatggtgcc
      301 gggtgcctcc cggtgaattc agtaccagca cctgaatccg cgattatccc atatacctac
      361 tcgctgattg cccctccgca cagggggatt caccatgcca gtttctttta acaaactccc
      421 cgcaaaccag acaacagtca accgcctgaa ttgtgagaca tttaaaaaaa aggcccgcaa
      481 aagcgagcca gggaaaataa gtgtggcgcg ttgtactgga ttcgaaccag tgaccgattg
      541 cttagaaggc aattgctctg tccggctgag ctaacaacgc aggatacaga taatggaccg
      601 ccttcgggga cccgaactcc gcgcaaccag cttcgaaagc tggcgctctt tcctgatgag
      661 ctaatggcgg tatgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg
      721 agtcgctcgc tctcaccact gagctaaagg gccgggtcaa aaaataataa tcagatgaaa
      781 atcaataatc aagcccttgc ctggatacat atctgtctgg cgggaagcca taatagcggt
      841 gaaatacaga aataaagtag gaccagcttg aataaccgca tttttctgct acagcctgtc
      901 catatccatg ccgggaacat aacatattca cagcaacacg catccgctct tccagcaaca
      961 agcgactgaa catgtgccct tcatttttca gttttatctt taacaaactc tcactcatat
     1021 gcaggcgtaa cgcaatcgca ccaagcgtcc agcttgctga tatatctgtc tgaattatcg
     1081 ccctgacttt ggcacttata ctggataaac atccacttaa aaataataat atccgttcat
     1141 ctgattcaaa cagcgacagg catgccatca taagaaacat atccgtggta tctccggaaa
     1201 atctctggct ggtaattaaa gcctcagcca acgcagggtt gttgggttcc agtgacagat
     1261 aaagcggaac gtcagtcaga tgagttcttg tcagcttatg ctgaatttcc agatattgac
     1321 ttactatgga atggtttata tcgaaaattt taactttgcc ataatgcata aggaaaagcg
     1381 ccctgatgca tttggtggcc agaacgactg agccgggctt aagtgacaac gtatcctttt
     1441 caagaaaaat attaattggg gagcaaacca tgataactga acagacaaca accattataa
     1501 ttttactttc attagcaatt ggttagttca attatagccc caaaaggtaa attatcatca
     1561 acacataagc aaaggactga caggtgccgc taacacccac cagccgccca tttaccacaa
     1621 ataaaaaagc cttcaggact gaaggcgtct gtaacaaccg cactgatagt ctgccagacc
     1681 cgccataaca agctgggtca gtattaactg gcagcgttcg cgtgaaaggt aagtattctg
     1741 cgcaatctcc ccgactgtcg ccggttcggt gacgcttaat tcatcaaaca caactctggc
     1801 ggtttctgtc atatcctgct gtttcagcat gtctttttac cctttccggt taacgtgaca
     1861 caccaataac tcttgccgaa aaagccagca agctgaaaga cctgtattaa taactaccag
     1921 cacatttaac gcactgcgct actttgcggg cacaaaaaac ccgctcagag gcggggtcaa
     1981 gctatgcggc gaaataacca ctcttaacag catacctgat tttttacgta cgtaaatgct
     2041 ttgccgtgca tatttttcat gcaaatgtca cgtcctgcta tttttcagtc ttataaattt
     2101 aaaaccatag aaaaaatcaa ttatgtttta aaaatggata ggtaaagaat aacaagtgac
     2161 acagattcaa accaaaatgg aaaagggtgg caacccacaa acgcccactc cacatccatg
     2221 acaatccata cacaacacca gataacgtgg caaataatac aagtaaagca ccacctgaat
     2281 agtgataaaa accaaacaac aaagccgcca caattaatgc aaccaatgga gacgttactt
     2341 ctgatagccg tgattgaata taccctctaa ataatgattc ctctgccaga gacacaaaaa
     2401 acaaattagc caatataaac tctggcaacc actcaggaaa atgaatctct ggctttaatc
     2461 caccaaaaaa aacagccaaa aacaggataa gaggaacaga gagcgacaga gccccccact
     2521 tccacaaaga cacttctgat tttacttctt ttttaaacaa agaagatgta cacaggacta
     2581 acaaaaatgg caccagcgct ttatcaaaat taaaatacat tgtataggga gtactctgag
     2641 ggccaacagt aacagaattt agcactacag gattgtgaaa ccctggccat agatggaaag
     2701 ataatgctat ggctgataaa actatgccaa cttcatatat agatttagcc caggcgttat
     2761 atttccagtt gaacttcaaa ataataaaaa aaacgattgt agcaacaaaa aataacaccg
     2821 accaatcaat aatatcatta agcacagcca ggacaacaga caccgtcaac aatgaaaaag
     2881 ccactacctt atgccaactg agagttgaca gtgacagtac taacacaatc cacataagca
     2941 ctccttttat ttaatgatga agattgatta tcaatatttt caattcacca ggcaacattt
     3001 tatctacctt ccacaataca tgaccatcaa aaaataaaca tgttaattat aaacacagaa
     3061 aacataaccc tcatcactat atatccctac cgcatatcca tatctaaccg gacattcaga
     3121 gccataagca ttccttcaat aataccttcc gctttataaa gccttttacc aataagccca
     3181 tcagaacatc tatgcttacg tgcaagagcc ataaatgtca ttccacctac gtaataatcc
     3241 accaacaaat cgtgcaaata ctgattattc ttgtttaatc gtgccataca accacaaatt
     3301 atcatggcgt catcatcaga acactgagga cgtgatttca ctttagtcgg gattaatcct
     3361 ttaaaaccag cagctatcga gggccatgtt acatcttcat gattatttgc tgcccatgcc
     3421 ccccatcgct caagaaccat ctggatatca cgcgccatcg ttaccacctc tgatttcgta
     3481 aatcttcacg cccagccgtc cacctggcac aggctgaccg cgcacaatat tgatttcatc
     3541 aaactgctcg tcatcaatga gcacttccgc atgcgtcagc gcatccagcg gtgctttcag
     3601 aatgttgtcc aggtcacggc ggcgcttatc cggtggttct gcaataattt ttattgccag
     3661 ccgtccggac aggcttaatt tcagccgctg ctggcgaaca ataagcgcca cagcccggcg
     3721 ataacgctca ccggcttttg atacaaaata tgtgctgcca cgacgtcgcc agtaggtgtt
     3781 caccgtcggc gggtaaggca aaacaaactc tatacgcatc agtaacctct tttacccgag
     3841 cacgccggtt gcaaaagcgt gatcaagaaa acgaaaaatt aaatcaacct gggaaccatg
     3901 cttttcttcg aacgccagcg gatccgcatg aagctcgttg tgatgctccc gacacagcgg
     3961 tagcgtgaaa atatcgtggg attttgttcc cattcc
//
LOCUS       DAZFYC010000238         3951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFYC010000238 DAZFYC010000000
VERSION     DAZFYC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.243"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..894)
                     /locus_tag="ACHKW5_005145"
     CDS             complement(<1..894)
                     /locus_tag="ACHKW5_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7514314.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGNTKDTNTKENRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AVVTASKPKLDLTNTDWIYGVD"
     gene            complement(927..1220)
                     /locus_tag="ACHKW5_005146"
     CDS             complement(927..1220)
                     /locus_tag="ACHKW5_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO7514315.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(1340..1555)
                     /locus_tag="ACHKW5_005147"
     CDS             complement(1340..1555)
                     /locus_tag="ACHKW5_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7514316.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            1659..2291
                     /locus_tag="ACHKW5_005148"
     CDS             1659..2291
                     /locus_tag="ACHKW5_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7514317.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGNGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            2381..2692
                     /locus_tag="ACHKW5_005149"
     CDS             2381..2692
                     /locus_tag="ACHKW5_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514318.1"
                     /translation="MNGDRYEFTKDSTEDSVFNVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVETWSITTDKKALYSKVMNIPGMQQLTSTKSFVGDVVGTCN
                     Q"
     gene            2912..3367
                     /locus_tag="ACHKW5_005150"
     CDS             2912..3367
                     /locus_tag="ACHKW5_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO7514319.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTDRRIQ
                     MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKPKSRVESALNPIDLT
                     VLAEYHEQIESNLQRIERKNHRVWYSKPGEFGITCQGRQKVKGESIPLA"
     gene            3376..>3951
                     /locus_tag="ACHKW5_005151"
     CDS             3376..>3951
                     /locus_tag="ACHKW5_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514320.1"
                     /translation="MKKIDYKSIPKPIDSASERKKHKKEAEKLANYISFIRNNAHGDG
                     DKKLLSDARTQAFGILRKQMQYRLHPGYIIEIRPTERQLFLLNSVFDFVNVVGDLIDR
                     SVDKDPDTNSFLLTNKEYLYGKFGINGWQKYVRFLRAFVDAYKNSDMIYTYLPGGDNC
                     TLSADNRIFIPVLGLGLINAVNEKDVIIVKQW"
BASE COUNT         1086 a          858 c          889 g         1118 t
ORIGIN      
        1 atccaccccg taaatccagt ctgtgtttgt caggtcgagt tttggtttgc tggctgtcac
       61 gactgcctgt tgcttgttac ggttgatttc gagctgggtc cacttgtcgc ggagtttggc
      121 cgggctcagc acattaccgg accagaagtt gtcctggcag gcccagcgga acagcacaca
      181 catatcgcgg tggttacgtc cgtcacgttc acgcatcagg cggatatcgt tagcccaccc
      241 tgcaaaattc ggttttctgg ctgatggcgc gatagtcttc accatgtcaa acatccactc
      301 tgcggcggtc aggtcttctg ctgtccccca tttgctgccg ctctgaattg cagcatcagg
      361 tttcaccaca gaaaggtcgt tttctggctg gtcagaggat tcgccagaat tctctgacga
      421 ataatctttt ctattttctt ttgtattagt gtcttttgtg ttcccctgtt ttgagggata
      481 gcaatccccc aatttgaggg atgttttatc cctcgtttta ggggattttc cctcgttttg
      541 agggatacac cattctgaga tgtttttatt tggtccaaac atgccgcctt gctgcttgat
      601 aatattcatt ctgacgagtt ctaacttggc ttcattgcac cgtttgacag gtaactttgt
      661 aatctcgcta agttgagaat cggtgattct gtccattggt ttattccacc cataggtttt
      721 acgcagaatg gcaagcagca ctttaaactg tcgcttggtc agatctgcgc ctgaataagc
      781 ctcaagcagc atatttgata gtctggcgta accatcatcg agatctgcca cattacgctc
      841 ctgtccggca aagttacctc tgccgaagtt gagtattttt gctgtatttg tcataatgac
      901 tcctgttgat agttccagta ataacctcag aattccatct ggatttgttc agaacgctcg
      961 gttgccgccg ggcgtttttt attggtgaga atcgaagcaa cttgtcgtgc caatcgagcc
     1021 atgtcgtcgt cgacgacacc ccattcaaga acagcaagca gcattgagaa ctttggaatc
     1081 caatccctct tccacctgct gatctgcgac ttatcaactc ccacagcttc cgctgtcttc
     1141 tcagttccaa gcattgcgat tttgttaagc aacgcactct cgattcgtag agcctcgttg
     1201 cgtttgtttg cacgaaccat atgtaagtat ttccttagat aacaattgat tgaatgtatg
     1261 caaataaatg catacaccat aggtgtggtt tattttgatg ccctttttca gggctgggat
     1321 gtgtaagagc gggaatgtct taagcggctt taccgcgttt agttccgtac tgtaaccaaa
     1381 ccggatcaca gttaagcgcc atagcaatct caaacaagaa gcgcggtcgc ttggttactc
     1441 cagcttcaat cagttgaatt gattgctgtt taacaccggc tttggttgcc agttcggttt
     1501 gcgtcatttt taacgcaatt cgcctcttct tgaggcgttc agaaagagtt tgcatatcgc
     1561 ctccatcaac aaactttctt gtattttcat acaatgtatc ttgtttgtca aatacagttt
     1621 ttcttgtgaa gattgggggt aaataacaga ggtggcttat gagtatttct tccagggtaa
     1681 aaagcaaaag aattcagctt ggacttaacc aggctgaact tgctcaaaag gtggggacta
     1741 cccagcagtc tatagagcag ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag
     1801 aacttgcgtc agctcttggc gtaagtgttg actggctgct caatggcacc tctgattcga
     1861 atgttagatt tgttgggcac gttgagccca aagggaaata tccattgatt agcatggtta
     1921 gagctggttc gtggtgtgaa gcttgtgaac cctacgatat caaggacatt gatgaatggt
     1981 atgacagtga cgttaactta ttaggcaatg gattctggct gaaggttgaa ggtgattcca
     2041 tgacctcacc tgtaggtcaa agcatccctg aaggtcatat ggtgttagta gatactggac
     2101 gcgagccagt gaatggaagc cttgttgtag ccaaactgac tgacgcgaac gaagcaacat
     2161 tcaagaaact ggtcatagat ggcggtcaga agtacctgaa aggcctgaat ccttcatggc
     2221 ctatgactcc tatcaacggg aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa
     2281 aattcgtatg atcaggattg cggcgctact ctcaatactc ttaactacca gcgccaattc
     2341 tgaatgctgg attgtcacaa acctgcacgg gtacggggca atgaatggcg atcgttacga
     2401 gtttacaaaa gacagcacgg aagattccgt tttcaacgta acaataaatg gcgataaatc
     2461 atcagtttat gaatcagttt ctggcgtcta tccagagatg aaatacactg ctttgtcatc
     2521 gaacactatg gtaggagaat accagtctgg aggaggcata accgttgaaa cttggtcaat
     2581 cactacagac aaaaaagctc tttactccaa agtaatgaac atcccaggta tgcaacaact
     2641 tacatcaacc aaatcctttg ttggtgatgt agtcggaacc tgcaatcagt aatccccacc
     2701 tcaatctcga taacaaaaaa acaaactatt ttccgtttaa aaacaatgga gtttgttttt
     2761 cacgcccctt tttacaatat ttcttgttta caacatacaa tcttccttgt aattttaagc
     2821 catcagcagg acgcactgac caccattgaa ggtgaggctc ttaaaaatta agccctgaag
     2881 aagggcagca ttcaaagcag aaggctttgg tgtgtgtgat acgaaacgaa gcattggccg
     2941 gaagtgcgaa tccggattag ctgccaatgt gccattgcgg ggtgttttcg ttcaggacta
     3001 cgactcccac acacaaccaa agctaactga caggagaatc cagatggatg cacaaacacg
     3061 ccgccgcgaa cgtcgcgcag agaaacaggc tcaatggaaa gcagcaaatc ccctgttggt
     3121 tggggtaagc gctaaaccag ttaaccgccc tattctctcg ctgaatcgca aaccgaaatc
     3181 acgagtagaa agcgcactga atccgataga ccttacggtg ctggctgaat accacgaaca
     3241 gattgaaagc aacctgcaac gtattgagcg caagaatcat cgagtttggt atagcaagcc
     3301 aggtgagttc ggtataactt gtcaaggaag acaaaaggtt aaaggggaat ccattccatt
     3361 ggcatgaggt acgtaatgaa gaagattgat tacaagtcca taccaaaacc aatagactca
     3421 gcatcagagc gaaaaaaaca caaaaaagag gctgaaaaat tagcaaatta tatcagtttt
     3481 attagaaaca atgctcacgg cgatggcgac aagaagttgc tttcagatgc tcggacccaa
     3541 gcgttcggta tacttcgtaa gcagatgcaa tatcgtcttc atcctggcta cataattgaa
     3601 attcgcccta ctgaaagaca attgttctta ttaaactctg tctttgactt tgtaaacgta
     3661 gttggagatc ttatcgacag gtctgtagat aaggatcctg atacaaatag ttttcttcta
     3721 acaaataaag aatacttata tggtaaattc ggtataaacg gatggcaaaa atatgtacgc
     3781 ttcttacgtg cattcgttga tgcgtataaa aattccgaca tgatttatac atatttgcct
     3841 ggtggtgata attgtacgct ttcggcagac aatagaatat tcatacctgt acttggtctt
     3901 gggctgatta atgcagtaaa tgaaaaagat gttataattg ttaagcagtg g
//
LOCUS       DAZFYC010000239         3917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFYC010000239 DAZFYC010000000
VERSION     DAZFYC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.244"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..264
                     /locus_tag="ACHKW5_005152"
     CDS             <1..264
                     /locus_tag="ACHKW5_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7514321.1"
                     /translation="TEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAAL
                     RISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            261..467
                     /locus_tag="ACHKW5_005153"
     CDS             261..467
                     /locus_tag="ACHKW5_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO7514322.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            464..2065
                     /locus_tag="ACHKW5_005154"
     CDS             464..2065
                     /locus_tag="ACHKW5_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7514323.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTSDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            2046..3365
                     /locus_tag="ACHKW5_005155"
     CDS             2046..3365
                     /locus_tag="ACHKW5_005155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7514324.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            3375..3707
                     /locus_tag="ACHKW5_005156"
     CDS             3375..3707
                     /locus_tag="ACHKW5_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7514325.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            3763..>3917
                     /locus_tag="ACHKW5_005157"
     CDS             3763..>3917
                     /locus_tag="ACHKW5_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7514326.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMAL"
BASE COUNT          870 a         1066 c         1221 g          760 t
ORIGIN      
        1 accgaagcgc agcaactgac tgctgaagag caggtcgaaa aatgggtgga tggcaggaaa
       61 aaaatactgt gggacagcaa aaagcgacgc aatgaggcgc tcgactgctt cgtttatgcg
      121 ctggcggcgc tgcgcatcag tatttcccgc tggcagctgg atctcagtgc actgctggcg
      181 agcctgcagg aagaggatgg tgcagcaacc aacaagaaaa cactggcaga atacgcccgt
      241 gccttatccg gagaggatga atgacgcgac aggaagaact tgccgctgcc cgtgcggcac
      301 tgcatgacct gatgacagga aaacgggtgg caacggtaca gaaagacgga cggcgagtgg
      361 agtttacgac cacttccgtg tctgacctga aaaaatacat tgctgagctg gaagtgcaga
      421 ccggcatgac acagcgacgc aggggaccag caggatttta tgtatgaaaa tgtccaccat
      481 tcccaccctt ctggggccgg acggcatgac atcgctgcgt gaatatgccg gttatcacgg
      541 cggtggcagc ggatttggtg ggcagttgcg ggcgtggaac ccaccgggtg aaagtgtgga
      601 tgcagccctg ctgcccaact ttacccgtgg caatgcccgc gcagacgatc tggtacgcaa
      661 taacggctat gccgccaacg ccatccagtt gcatcaggat catatcgtcg ggtctttttt
      721 ccgactcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
      781 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaagatgact gttgctgcat
      841 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
      901 ctttaacggt gaactgttcg ttcaggccac ctgggatacc cgtccctcgc gactgttccg
      961 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccagcgacag
     1021 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     1081 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgcgagtt
     1141 acccggcggg cgcgcctcgt tcattcatgt ctttgaaccc gtggaggacg ggcagacccg
     1201 cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa
     1261 cacgcagctg cagagcgcca ttgtgaaggc gatgtatgcc gccaccattg agagtgagct
     1321 ggatacgcag tcagcgatgg attttattct gggcgcgaac agtcaggagc agcgggaaag
     1381 gctgaccggc tggattggtg aaattgccgc gtattacgcc gcagcaccgg tccgtctggg
     1441 aggcgcaaaa gtgccgcacc tgatgccggg ggactcactg aacctgcaga cggctcagga
     1501 cacggataac ggctactccg tgtttgagca gtcactgctg cggtatatcg ctgccgggct
     1561 gggtgtctcg tatgagcagc tttcccggaa ttacgcccag atgagctact ccacggcacg
     1621 ggccagtgcg aacgagtcgt gggcgtactt tatggggcgg cgaaaattcg tcgcatcccg
     1681 tcaggcgagc cagatgtttc tgtgctggct ggaagaggcc atcgttcgcc gcgtggtgac
     1741 gttaccttca aaagcgcgct tcagctttca ggaagcccgc agtgcctggg ggaactgcga
     1801 ctggataggc tccggtcgta tggccatcga tggtctgaaa gaagttcagg aagcggtgat
     1861 gctgatagaa gccggactga gcacctacga gaaagagtgc gcgaaacgcg gtgacgacta
     1921 tcaggaaatt tttgcccagc aggtccgtga aacgatggag cgccgcgcag ctggtcttaa
     1981 accgcccgcc tgggcggctg cggcatttga atccgggctg cgacaatcaa cagaggagga
     2041 gaagagtgac agcagagctg cgtaatctcc cgcatattgc cagcatggct tttaatgagc
     2101 cgctgatgct tgaacccgcc tatgcgcggg ttttcttttg tgcgcttgca ggccagcttg
     2161 ggatcagccg cctgacggat gcagtatccg gcgacagcct gactgccgga gaggcacccg
     2221 cggcgctggc gttatccggt gatgatgacg gaccacgaca ggcccggagt tatcaggtca
     2281 tgaacggcat cgccgtgctg ccggtgtccg gtacgctggt cagccggacg cgggcgctgc
     2341 agccgtattc gggaatgacc ggttacaacg gcattatcgc ccgtctgcaa caggctgcca
     2401 gcgatccgat ggtggacggc attctgctgg atatggacac accgggcggg atggtggcgg
     2461 gagcatttga ctgtgctgac atcatcgccc gtgtgcgaga cataaaaccg gtatgggcgc
     2521 tggccaacga catgaactgc agtgcaggtc agctgcttgc cagcgccgcc tcccggcgtc
     2581 tggtcacgca gaccgcccgg acaggctcca tcggcgtcat gatggctcac agtaattacg
     2641 gtgctgccct ggagaaacag ggcgtggaaa tcacgctgat ttacagcggc agccataagg
     2701 tggatggcaa cccctacagc catctaccgg gtgatgtccg ggaaacactg cagtcccgga
     2761 tggatgcaac ccgccggatg tttgcgcaga aggtgtcggc atataccggc ctgtccgtgc
     2821 aggctgtgct ggataccgag gctgcagtgt acagcggtca ggaggccatt gatgccggac
     2881 tggctgatga acttgtcaac agcaccgatg cgatcaccgt tatgcgtgat gcactggatg
     2941 cacgtaaatc ccgtctctca ggagggcgaa tgaccaaaga gactcaatca acaactgttt
     3001 cagccactgc ttcgcaggct gacgttactg gcgtggtgcc agcgacggag ggcgaaaacg
     3061 ccagcgcggc gcagccggac gtgaacgcgc agatcaccgc tgcggttgcg gcagaaaaca
     3121 gccgcattat ggggatcctc aactgtgagg aggctcacgg acgcgaagaa caggcccgcg
     3181 tgctggcaga aacccccggt atgaccgtgg aaacggcccg ccgcattctg gccgcagcac
     3241 cacagagtgc acaggcgcgc agtgatactg cgctggatcg tctgatgcag ggggcaccgg
     3301 caccgctggc tgcaggtaac ccggcatctg atgccgttaa cgatttgctg aacacaccag
     3361 tgtaagggat gtttatgacg agcaaagaaa cctttaccca ttaccagccg ctgggcaaca
     3421 gtgacccggc tcataccgca accgcgcccg gcggattgag tgcgaaagcg cctgcaatga
     3481 ccccgctgat gctggacacc tccagccgta agctggttgc gtgggatggc accaccgacg
     3541 gtgctgccgt tggcattctt gcggttgctg ctgaccagac cagcaccacg ctgacgttct
     3601 acaagtccgg cacattccgt tatgaggatg tgctctggcc ggaggctgcc agcgacgaga
     3661 cgaaaaaacg gaccgcgttt gccggaacgg caatcagcat cgtttaacct gacccttcat
     3721 cactaaaggc cgcctgtgcg gcttttttta cgggattttt ttatgtcgat gtacacaacc
     3781 gcccaactgc tggcggcaaa tgagcagaaa tttaagtttg atccgctgtt tctgcgtctc
     3841 tttttccgtg agagctatcc cttcaccacg gagaaagtct atctctcaca aattccggga
     3901 ctggtaaaca tggcgct
//
LOCUS       DAZFYC010000240         3916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFYC010000240 DAZFYC010000000
VERSION     DAZFYC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.245"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..518
                     /locus_tag="ACHKW5_005158"
                     /pseudo
     CDS             18..518
                     /locus_tag="ACHKW5_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            520..813
                     /locus_tag="ACHKW5_005159"
     CDS             520..813
                     /locus_tag="ACHKW5_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO7514327.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(874..1994)
                     /locus_tag="ACHKW5_005160"
                     /pseudo
     CDS             complement(874..1994)
                     /locus_tag="ACHKW5_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(2003..2770)
                     /gene="sctT"
                     /locus_tag="ACHKW5_005161"
     CDS             complement(2003..2770)
                     /gene="sctT"
                     /locus_tag="ACHKW5_005161"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO7514328.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(2772..3032)
                     /locus_tag="ACHKW5_005162"
     CDS             complement(2772..3032)
                     /locus_tag="ACHKW5_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7514329.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIEVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(3042..3707)
                     /locus_tag="ACHKW5_005163"
     CDS             complement(3042..3707)
                     /locus_tag="ACHKW5_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7514330.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(3697..>3916)
                     /locus_tag="ACHKW5_005164"
     CDS             complement(3697..>3916)
                     /locus_tag="ACHKW5_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO7514331.1"
                     /translation="VKIEFVLGKKIMNLYEIDDLCAKRIISLLPESEKNIEIRVNGAL
                     TGYGELVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT         1331 a          772 c          620 g         1193 t
ORIGIN      
        1 agagtacagg tgggcatatg caagtctttt ccagtgatgt ctacttcact gtaggcacta
       61 atgcattatt agcatcccaa aaagaatatt acagcgattt agttgcgttg gttgatcttg
      121 gacattcgtt cgttgttatt gatgagcatc aacatcgaaa tctcaaacca aatacaaaac
      181 ctgtaaacat ccttttgagc aacaatttca tcaggatcaa taagaatatt acattatctg
      241 atttaacgca tttcctggtt tcaaatcttc atacacaaaa tgtgtattcc acacaagaac
      301 cacttatcca tgatgagata gatatcctgc ggttatgtgt ttcttactcg ttaaaacaaa
      361 tagccattat taaaggaatt gattataaaa caatatcata tcacaaaata agagctctaa
      421 ataaacttaa cataaaaggt actgtttagt tgtttatagc actctgtgaa tgggataaac
      481 attattttaa actccaatct tgcgtaagtg aaagttgata tgtcattagt aggatataat
      541 agtaacatca taataaccat cgcgctgata tcgttgctct ctgcatgcac cagagaaaat
      601 tcattaaagg agaatcaaaa taaaagaagt ggcgaatccc aagcgatagt agatgcaaga
      661 cagttcttta gcgaacaccc ggaatatata tcatcagcag aactggagca aaagctatat
      721 cgcgaatttg cacaggttac tacagttcca gcttataaag agatgagtat gtatcaattg
      781 cttatcattt cacaagatcg tttaactcat taaataagga tctacacgct ttttgcgtgt
      841 agatccttat aaatttctga ctacgacaga tgtttaattt tttaaattat cgtctttatt
      901 ctcgattttt gtgtcttcac cctcaatgaa tttatcttct gaagagagct gttcatcttg
      961 aacaggttgc ccagcattct ccacatcttc aagccacagc agaagacgta atatttcatc
     1021 tatattttcg aagctaacat aatcgtagcg acgatgggta gcataatttt ttcgtgctaa
     1081 ttttttatct gtgataattg gtattccgat ttcctttgca tatttcctta cgcctaaggc
     1141 cacctcatta gttactctta cagaaatcaa tggaataggt gaaagatgtg gcttaaagta
     1201 tatccctatt gcaatgtgag tagggttgga aatcatcaaa cgactattac tgacatcaga
     1261 cttcaattgc tcagaaagaa tttcctgatg cgctctctgc gtttagactt aatttctgga
     1321 tttccttctt gctccttgta ttctctttta acttcttgtt tatccatttt catatctttc
     1381 ataaataaaa aatattcagc gataaaatca aaaattaaga caattatcat actgccgaga
     1441 caatacagta tgagaaggaa tagcatctct ccccaatctg ataataaaga tatgatatct
     1501 ccatcaagag ttttaaaaag cagtgattta tgattactcc aaaatacttt gatcgccaat
     1561 gcaaaaaaaa caatataaag aattgcttta acaaattctt ttacggtttt taagccaaat
     1621 atacgcttta agccatttac tggattcaat gaatcaaact taaatttaac agcttcagtt
     1681 gctaattgca ttttactttg aaaccaggag acaagtattg ttgtgaacat gcaaaccagc
     1741 aaaaaaggaa tgttcgtttt caaccaacca acccccatag ctttaaaata gtcccaaata
     1801 tctggtttag cgttatgatc aaggatgtat tcaagaatcg acataatgtg atgcacatca
     1861 aaaacatatc cgagatataa agtacccaca agcattatta cagagatggt taagtctcta
     1921 ctttttagaa tttgcccttt tttagaagca tcctggagtt tcttttgtgt tggcttttca
     1981 gttttattgg ccatgaaaca ctctatttct caatgtatag ttgcaattgt tctgggaaaa
     2041 aggataacgg cattactcgt tcagccagta ttggggcaaa ataaataatt aatattaaaa
     2101 atgccagcgc acttttgaca gtcagtgaaa tggcaaaagc atttagttgt gaagcataac
     2161 atgccagcaa tcccaggaca gcctcaccgc ccagcataac ggcaatcacg gggcttgcgt
     2221 aaacgatagt atgagtcatt atgttattaa tatatgagaa taaaggctct aatttaggaa
     2281 aattaaaatt gcctgaaggc cataaattat aactttgcca aagagtttcc agaataaagt
     2341 tcattccacc tttagtaaga taaaccgcag cagagaaaag gttaaataat cttgctaatt
     2401 ccgaggtatc gacacctgta gccggatcca aagtactact taatgttgca ccgcgttggt
     2461 tatcgataaa gctacctata gcaaggaaca tccaaaacgg ggatgctaat aagcatgcaa
     2521 taaacagccc tatgataatt tctctggcag cgaacaaagc aatatcgatt tcatttaagt
     2581 tcaataattc atagtggtaa tgctgaacta atgcacatga taccactatt attattgggt
     2641 gacgaactac tgttggtata ttcccactgg cgaaaaatgg taacaaatag aaagttggtg
     2701 ccaatcggca aaaaccgagc gctgtagcag ccagcagact atgcaactga tagagtatcg
     2761 cctcccccat gctatcctct cgcgaatgct aaattcagca tttcgatacc aaatgaatat
     2821 aatttttcac cataccaccc gctcattaaa aaaaagcaaa tacttacgca caataatttc
     2881 acgccaaatg gcaacgtctg ttcttgcaac tgcgttaccg tttgaaaaag ccctaccaat
     2941 aaaccaacaa atgtcgcaac ttcaataggt cctgcagaca taactaaaat cagatacagc
     3001 gccctattcc ccgcaaaaac aatatcgtcc atattttttc ctcagggatt aatggatagg
     3061 tcaaaatatt gtaaaatgag cccttttgat aacattgtcc aaccgtccat cgcgacaaac
     3121 aaaattaatt ttatcggtgt agatatggtt accggactca tcatcatcat acctaacgtt
     3181 aacagaacgc tggatataac cagatccact acgacaaagg gtaaatagat ataaaaacct
     3241 ataataaatg cactttttat ctcactcaat gcatatgcgg gcagtaacga gaaaattgaa
     3301 atattatcat catcgattat ctcttcattg tcttcagaac tattcacctt ctgaatcttt
     3361 tcaaaaaagc taaccaactc aggttcagaa tattttatca aatatgattt ataaccactc
     3421 atgcctgtct caacaaaatt aacaaccgat gctacattat tgaatgaaag attctcattc
     3481 tgactgttat tatatatttc cttcccaacc ggcatcatta cgaacattga caatagcaaa
     3541 gcaaccccat taagtgtcat atttgatggc acttgctgaa gtcccagcgc gtttcggaca
     3601 ataacaaaaa caatcgagaa tttaataaaa catgtcccgg atgctattat aaacggcaat
     3661 agtgtaaata atgataatat cgctatcagc gaaatgctat ttgacattat tattccctga
     3721 taaccaagaa tgaatttcta cgcccaattt atcatccact tcaacaagtt cgccatagcc
     3781 agttagcgca ccatttacac gtatctctat atttttctca gattcaggaa gcagagatat
     3841 tattcttttt gcacagagat cgtctatttc atatagattc attattttct taccaagaac
     3901 aaactcaatt tttaca
//
LOCUS       DAZFYC010000241         3894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFYC010000241 DAZFYC010000000
VERSION     DAZFYC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.246"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            35..1693
                     /gene="dpiB"
                     /locus_tag="ACHKW5_005165"
     CDS             35..1693
                     /gene="dpiB"
                     /locus_tag="ACHKW5_005165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO7514332.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSMRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1662..2342
                     /gene="dpiA"
                     /locus_tag="ACHKW5_005166"
                     /gene_synonym="citB"
     CDS             1662..2342
                     /gene="dpiA"
                     /locus_tag="ACHKW5_005166"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO7514333.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2383..3768)
                     /gene="dcuC"
                     /locus_tag="ACHKW5_005167"
     CDS             complement(2383..3768)
                     /gene="dcuC"
                     /locus_tag="ACHKW5_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7514334.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1061 a          930 c         1013 g          890 t
ORIGIN      
        1 tcaaagaggc tcgggacaac ccgcaaggaa aacaatgttg cagcttaacg agaataaaca
       61 gtttgcattt ttccaaagac tggcatttcc gctgcgtatc tttttgctga ttctggtgtt
      121 ctcaatattt gtcattgcag ccctggcgca atattttacg gccagttttg aggactattt
      181 aacgcttcat gtacgcgaca tggcaatgaa tcaggcgaaa attattgcct ccaatgacag
      241 tgtcatctct gcggtgaaaa cgcgtgacta caaacggctg gcgaccatcg ctaacaaatt
      301 acaaagagat accgattttg attatgtggt gattggggac cggcactcga tccgccttta
      361 ccatcctaat ccggagaaaa ttggttatcc tatgcagttc accaaacagg gcgcgctgga
      421 gaaaggggag agctacttca ttaccgggaa agggtcaatg gggatggcga tgcgcgccaa
      481 aacgccaatc tttgatgacg atggaaaagt catcggcgtg gtgtcgattg gctacctggt
      541 gagtaaaatc gatagctggc gggctgagtt tttattaccg atggcaggcg tgtttgtcgt
      601 gctgttaggg attctgatgt tgctgtcgtg gttcctggca gcgcatatcc gtcggcaaat
      661 gatgggcatg gagccaaagc aaatcgcacg cgtggtccgt cagcaagagg cgctgtttag
      721 ttcggtttat gaagggctga ttgcggtgga tccgcatggt tacattaccg ccatcaatcg
      781 taacgcaaga aagatgctgg ggctgagctc tcccggacgg caatggttgg gtaaacccat
      841 tgctgaagtg gtcaggcccg ccgatttctt taccgaacag attgatgaaa aacgtcagga
      901 tgtggtggct aactttaacg gtctgagcgt tattgccaat cgggaagcta ttcgttcagg
      961 tgatgatttg ctgggggcca ttatcagctt tcgtagtaaa gacgaaattt ccaccctcaa
     1021 tgcgcaactg acgcaaataa aacaatacgt cgagagcctt cgtacattgc gacacgagca
     1081 tctcaattgg atgtcgacgc tcaatggtct gttgcagatg aaagagtatg atcgcgtgct
     1141 ggcgatggtg cagggggagt ctcaggccca gcaacagctt attgacagcc tgcgtgaggc
     1201 gtttgccgat cgccaggtgg cggggctgct ttttggtaaa gtgcagcgcg cccgggaact
     1261 ggggctaaaa atgatcattg tccccggtag ccagctttcg caactgccgc caggactgga
     1321 tagcaccgag tttgcagcca ttgtgggcaa tttacttgat aacgccttcg aagccagcct
     1381 gcgtagcgat gaaggaaaca agatcgttga attattcctc agcgatgaag gcgatgatgt
     1441 ggtgattgaa gtcgccgatc agggctgcgg cgttccagag tctctacgag acaaaatatt
     1501 tgagcagggg gtcagtatgc gtgctgacga gcccggtgaa catggcattg ggttgtactt
     1561 gattgccagc tacgtaacgc gctgcggtgg tgttatcact ctcgaagata atgatccctg
     1621 cggtaccttg ttttcaatct atattccgaa agtgaaacct aatgacagct ccattaaccc
     1681 tattgatcgt tgaggacgaa acgccgctgg cagagatgca tgcggaatat attcgtcaca
     1741 ttcccggatt cagtcagata ttactggcgg gaaacctggc gcaggcccga atgatgatcg
     1801 agcgttttaa gccggggcta atcttgctcg ataactatct tcctgacggc agagggatta
     1861 atttattgca tgaactggtg caggcgcatt atcccggcga cgtggtgttt accactgcag
     1921 ccagcgatat ggaaacggtg tctgaagccg tacgttgtgg tgtatttgat tatctcatta
     1981 agcccattgc ctatgaacgg ctggggcaaa cgctaacccg tttccgccag cgtaaacata
     2041 tgctggaaag tattgatagc gccagccaga agcaaattga tgagatgttt aatgcctatg
     2101 ctcgcggtga acctaaagac gaactgccga ccggcattga tcccttgacg ctaaacgcgg
     2161 tgcgaaaact gtttaaagag cctggtgtgc aacatacggc agaaacggtg gcgcaggcac
     2221 tgaccatcag tcgcaccact gccaggcgtt atcttgaata ttgcgccagc cgccatctga
     2281 ttattgctga aattgttcac ggcaaagttg gcagaccgca acgcatatac cacagtgggt
     2341 gacatgataa agccggagga aacttgcctc cggcattact atttacttgc ctgtgaccgc
     2401 tgctgccgtt cctggcacca tcagctctgt agcaacgata acaatcacca aaccaacaag
     2461 caccggtacc gaggtgcgtt ttacgacttc aaacggcgag atcttcgcca tcccggcaac
     2521 cgcaacgact acgccagaaa cgggcgaaag ggtacggccc aggtttgacg cctgcagcat
     2581 cgggatagtc aaatacgccg ggttaatgcc ggaagagtgc gccagtttcg ggatcatctc
     2641 aacaaacgca taaaacggcg cattgcctga accggtcgtg actgccgcca gcatggtcag
     2701 aatcaccaat accagcatca ggatgatact cgccgaacca aacgaggtag cgatagaaat
     2761 cagactttga ataaagccga tggtgctaag cccctgagcg aataccccag cggcaaccag
     2821 cagcatcacc acgttagcaa acgcatctgc cattccgcga taagccactt ccagaccaga
     2881 gaaaactttc tgggtattaa agctgcggag gaactccaga atggaggcaa tcagcataca
     2941 aatcaccaga atagtgatga tgtgtaattg cggaccccat ttaccgtcaa aaatcagtac
     3001 accgatgatc ggcgtgaacg gcaaaatggc ataaaacgca ggagcagtgg tggtgatttc
     3061 actgacatct aacatttcat gagagatgtg ctctttttta tccagataac gttgccagaa
     3121 gaagtgggcg atcgccatgc cgataattgc agcaattgag ataggcagcg tcgttttgaa
     3181 ggcgaagtca atcagcgaca tttcggaagc ttgcgccgcc agcaccacat cccctgaagt
     3241 cggtgcgaga ataatcgccg ccggggaggc acaaatggca gcagctgcgc cacgactgat
     3301 accaacgttt accatcaccg gaaatagggt tgccatcagc aaaacaccca gaccggttgc
     3361 ggaagagacg gccagagaca tcagacaggc gacaaaatag gcggcaatca tcagcaggta
     3421 aggggagtta atatactgca atggttttga cgccagcttg accaccatat cattcgcgcc
     3481 gatatgggtc atgtaagcgg caaatccaca cagcatcata atcatcatgc cgaggtcgcc
     3541 gccgcggctc attagtaata ttttaacgta ttcaacgata tccgtggcgc tgtagcctgt
     3601 tgaagcctgg ctggacggta acactttgtg ccccataatg gcactgataa tcagcaataa
     3661 caggccaccg acaaataaca caccagtggc ggaataccct ttaatgatgt agcgagctac
     3721 acccacaata accacaaccc caataaggag ttcaatgaat gtcagcataa tttttcctgt
     3781 ctccaggccc caaagtaaat aataaaaaat tcctaaagct taaggaaaaa atatgcccaa
     3841 taaattggcg atgaatgctg attaaaatca agaaaaactg ccattaagac attg
//
LOCUS       DAZFYC010000242         3848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFYC010000242 DAZFYC010000000
VERSION     DAZFYC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.247"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            198..2477
                     /gene="ydeP"
                     /locus_tag="ACHKW5_005168"
     CDS             198..2477
                     /gene="ydeP"
                     /locus_tag="ACHKW5_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO7514335.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2725..2922
                     /gene="safA"
                     /locus_tag="ACHKW5_005169"
     CDS             2725..2922
                     /gene="safA"
                     /locus_tag="ACHKW5_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO7514336.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2997..3758
                     /gene="ydeO"
                     /locus_tag="ACHKW5_005170"
     CDS             2997..3758
                     /gene="ydeO"
                     /locus_tag="ACHKW5_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO7514337.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMNALAI"
BASE COUNT         1089 a          846 c          876 g         1037 t
ORIGIN      
        1 atatgttaat ttaaacacta tgagagatga tgcttaactt atcgtattta atctattgtt
       61 taacgataat agattaaaaa tagaaagaga gtagtcccct actcttatat atccatgttg
      121 gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt
      181 ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct gcaggtggtt
      241 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccaggatg
      301 ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc
      361 cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct
      421 gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg gttcaatcat
      481 tacttacctg gggagatcac gagcttgagg ctgcgggacg tctcactcag cctttgaaat
      541 atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg
      601 gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca
      661 cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
      721 ttcccgactg ctccaacatg tgccatgaac cgaccagtgt gggtttggca gcgagtatcg
      781 gcgtgggtaa agggactgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca
      841 ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga
      901 aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta
      961 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct
     1021 actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa
     1081 ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat gacgaattta
     1141 ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat tccgagtgga
     1201 aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg gctgacgcat
     1261 atgccgctgc cgaacgcacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1321 gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc
     1381 ctggtgcggg tatctgccca ctacgtggcc actctaatgt acagggcgac cgaaccgtcg
     1441 gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc tatggcttca
     1501 caccacctca agcacctgga catgctgcaa ttgccagcat gcaagcaata tgtacagggc
     1561 aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1621 cgagcgctgt accgttaacg caattagatc tggcggtaca cgtagccact aagcttaacc
     1681 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctggga cgtagcgaga
     1741 ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc
     1801 atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg
     1861 tcgcgggaat cgcgcaggca gcactacccc agagcgtggt agcctgggag tatctggtgg
     1921 aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc gccgactata
     1981 accaacgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct gaaaggcgct
     2041 ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct
     2101 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagccacgat cagtacaaca
     2161 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2221 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2281 ttgcgctcac gccagacggt aaacgtagct cacgccgcat ggatagatta aaagtggtca
     2341 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2401 cactcgataa ccacgatcca ttaagtggca ttcctggcta taaaagtatt ccggttgaat
     2461 tagaaccatc aaattaatgc ctcttctcat ttcttctgct gtcatccgca cagcagaaga
     2521 atccctcatt gactattatc gcgcaatttg ctcacatgga ttaaattaaa ctacatacta
     2581 taagatataa acctctgcct acagctgtaa gaaactccgc tcagtactga agtgccagtc
     2641 ctatttcctc ttttctccag cctgttatat taagcatact gattaacgat ttttaacgtt
     2701 atccgctaaa taaacatatt tgaaatgcat gcgaccacag tgaaaaacaa aatcacgcaa
     2761 agagacaact ataaagaaat catgtctgta attgtggttg tcttattact gacccttacg
     2821 ttgatagcca ttttttcggc aattgatcag ctgggtattt cagaaatggg tcgcattgca
     2881 agagatctta cacatttcat tatcaatagt ttgcaagact gataataaac agaaaattat
     2941 aaatctgaaa tgttaacaaa gtatcgataa aaactttatt gttttaagga gataaaatgt
     3001 cgctcgtttg ttctgtcata tttattcatc atgctttcaa cgctaacatt ttagataaag
     3061 attacgcctt ctctgacggc gagatcctga tggtagataa cgctgttcgt acacattttg
     3121 aaccttatga gcggcatttt aaagaaatcg gatttaatga aaataccatt aaaaaatatc
     3181 tacaatgcac taacatccag acagtgacga tgcctgttcc tgcgaagttt ttacgtgctt
     3241 caaatgtacc gactggattg cttaatgaaa tgattgctta tctcaactcg gaagaacgca
     3301 atcatcataa tttttcagaa cttttgcttt tttcttgcct gtctattttt gccgcatgca
     3361 aaggtttcat tacactatta actaacggtg tgctatccgt ttccgggaaa gtgagaaata
     3421 ttgtcaacat gaagctggcg cacccatgga agctgaaaga tatttgtgac tgcctgtaca
     3481 tcagtgaaag cctgttgaag aaaaaactaa agcaagagca aacgacattc tcacagattc
     3541 tattagatgc cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata
     3601 aaattgccga acaatgtggt tatgccagta catcttattt tatttatgcg ttccgcaaac
     3661 atttcggcaa cagtccgaag agagtttcta aggagtaccg ttgtcaaagt cacatgggta
     3721 tgaatacggg caacacgatg aatgctttag ctatttgatt atttgctaac gagtagtcaa
     3781 ccacgcaccc tacgtaaaaa ttaaaaatgg ggcagtcatt ccctgccccg cgttgttttt
     3841 aggtgata
//
LOCUS       DAZFYC010000243         3771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFYC010000243 DAZFYC010000000
VERSION     DAZFYC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.249"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..314
                     /locus_tag="ACHKW5_005171"
     CDS             87..314
                     /locus_tag="ACHKW5_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514338.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(391..633)
                     /gene="yfdY"
                     /locus_tag="ACHKW5_005172"
     CDS             complement(391..633)
                     /gene="yfdY"
                     /locus_tag="ACHKW5_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO7514339.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            986..1906
                     /gene="lpxP"
                     /locus_tag="ACHKW5_005173"
     CDS             986..1906
                     /gene="lpxP"
                     /locus_tag="ACHKW5_005173"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO7514340.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2262..2333
                     /gene="ypdK"
                     /locus_tag="ACHKW5_005174"
     CDS             2262..2333
                     /gene="ypdK"
                     /locus_tag="ACHKW5_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO7514341.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2398..3636)
                     /gene="alaC"
                     /locus_tag="ACHKW5_005175"
     CDS             complement(2398..3636)
                     /gene="alaC"
                     /locus_tag="ACHKW5_005175"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO7514342.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          974 a          952 c          887 g          958 t
ORIGIN      
        1 cgtaggttct cagtgaacca ttttcaggat aaatttctat gaaagtaaac ttaatacttt
       61 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
      121 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac
      181 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgcgccac
      241 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
      301 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
      361 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
      421 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacta gttcattacc
      481 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
      541 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
      601 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
      661 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
      721 gcatcaatga aaacagcatg ttaaatgcaa gactgttgtg tacggaaaaa tatttacttt
      781 gcacgattaa taatcagtag ctgaaagcag tcagcgagtg ataaagtaca gataccgcat
      841 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
      901 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
      961 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
     1021 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
     1081 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
     1141 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
     1201 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
     1261 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
     1321 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
     1381 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
     1441 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
     1501 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
     1561 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
     1621 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
     1681 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
     1741 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcctatat
     1801 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
     1861 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
     1921 tctttccctc ctcaaatcgg ccataataac cttatggttt ttatggcttt tttcttaagg
     1981 ttttttcctt cgctgaatat ctaattatta ccgtcagaaa ttaaactgtc gccgatatga
     2041 gacacttgta agttactaaa agtaaataag aaaatgtctc ttgtctcgtt tcagttttag
     2101 gcgtacatta gcgccgtctc gagcagttta gctcagaatt ctggggtgat gcccacggta
     2161 aaatacaggg cttttaaggt cgctttgccc ggtgtcaact cactgtatca ggtgtaatga
     2221 agtcattcag gcgtaacagt aattacgcgg agagatgtaa agtgaaatat ttctttatgg
     2281 gcatttcttt tatggtcatc gtttgggccg gtacttttgc cctgatgatc taaaagcaaa
     2341 ctgtgcaatg aaaaacagga gccatcaggc tcctgtttgc atttctgacg acggtgttta
     2401 ttccgcgttt tcgtgaatat gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat
     2461 cgctttaatc ccacgaatcg cctgacgaat acggtcgcgg ttttcgatca gtgcaaagcg
     2521 aacatgcgta tcaccatagt cgccaaagcc aatccccggc gagacacaaa ccttcgcttc
     2581 gttaaggagc ttcttggcaa attccagcga tcccatggcc gcatatggtt ccgggatttt
     2641 cgcccagaca tacatcgaag ccttcggcat ttcaaccatc cagcccgctt catgcagccc
     2701 tttaaccaat acatcacggc ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg
     2761 atcgccctcc agcgccgcaa tcgccgccac ctgcaacggc gtaaaggtgc cgtaatcgtg
     2821 atagctttta atacgagcca gggcgctgac cagcgttttg ttgccaacca taaagccgat
     2881 acgccagccc gccatgttgt agcttttcga cagcgtaaag aactcgactg ccacatcgcg
     2941 tgcacccggt acctgcatga ttgacggcgc tttccagcca tcgtagacga tatcggcata
     3001 ggccaggtcg tggaccacca gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa
     3061 gaactccagc tccacgcatt gcgcggttgg attcgacggg aagccgagga tcatcatctt
     3121 cggtttcgga taactttcac gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc
     3181 caccagcggc actgagcgta cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg
     3241 gtaacttgga ttcggcacca gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg
     3301 cgccaggccc tctttcgaac caatagtgac gatggcttct gattccgggt cgatttcaac
     3361 gtcgtagcga tcctgatacc agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga
     3421 agtggagtaa ccatgcgtgt ccgggcgctg ggccacagta cataattttt cgacgatatg
     3481 cggcggagtc gcaccgtccg ggttacccat gctgaaatcg ataatatctt cgccgcgccg
     3541 acgcgcagcc attttcagtt cagcggtgat gttaaaaacg tagggcggga gacgatcaat
     3601 gcgcgtaaag cgacgttcag ggcgagtgtc agccataatt tcctcagatt aacgttagcg
     3661 cccggaccgt ccgagcgacg ctgccacaat ggtggctgtt ttgaaaatag cctgattaat
     3721 ttctgtctgt ccagcgtttt agaacatttt tatcaatggc tttaaatgag g
//
LOCUS       DAZFYC010000244         3760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFYC010000244 DAZFYC010000000
VERSION     DAZFYC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.250"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..244)
                     /locus_tag="ACHKW5_005176"
     CDS             complement(29..244)
                     /locus_tag="ACHKW5_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7514343.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            387..785
                     /locus_tag="ACHKW5_005177"
     CDS             387..785
                     /locus_tag="ACHKW5_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514344.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTRLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(866..1024)
                     /locus_tag="ACHKW5_005178"
     CDS             complement(866..1024)
                     /locus_tag="ACHKW5_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7514345.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1110..1853)
                     /locus_tag="ACHKW5_005179"
     CDS             complement(1110..1853)
                     /locus_tag="ACHKW5_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514346.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(2106..2729)
                     /locus_tag="ACHKW5_005180"
     CDS             complement(2106..2729)
                     /locus_tag="ACHKW5_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7514347.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(2726..3391)
                     /locus_tag="ACHKW5_005181"
     CDS             complement(2726..3391)
                     /locus_tag="ACHKW5_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO7514348.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTQAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(3388..>3760)
                     /locus_tag="ACHKW5_005182"
     CDS             complement(3388..>3760)
                     /locus_tag="ACHKW5_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7514349.1"
                     /translation="IKQAQQAVNAFIRERDRDLPCISCGTLTSAQWDAGHYRTTAAAP
                     QLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEAVEEIESNHNRYRWTVEECR
                     AIKAEYQQKLKKLRNSRSEVA"
BASE COUNT         1069 a          931 c          724 g         1036 t
ORIGIN      
        1 gcaacggctt ttcgtaatga tggaggcatt attcacctct cgcagccttg cgcttatctt
       61 ctttaatctt gaaataaagg tttgtcagat acgtcagcag gccaaacagc agactcccca
      121 gcacacctat tgccacccac tgggacggag agactttgtc cagcagctgc agtaaccagt
      181 atcccgtccc caccgctgac gtggtgtatg acacacctgt tgtgattttt tccatctggt
      241 acataccccg tctcccgtta tccggaagct gacaacaata aaaagccacc agttaattcc
      301 tgatggccct gatgcataaa cgtcataata cctgactgtt atgattgaca ataatgataa
      361 tgtttatata gaaaggttcc cgatgtgtgt tacatatcat ttctccacgg ggaatatccc
      421 cacgccagcg cagactcttt tacccgttct cttctgcgct ggctcttttt tattatgctg
      481 ctgcatttac ctctggcacc aggctttcta tctcaacaca atacgtggta cttcttgtaa
      541 ccaatatcat aacgattaat cgacatagaa tttctcccgt gtacaggaac agagttaaaa
      601 agccggaacc ggaatcaaat cacaggatga ccatctgcca gtggcaggtc ataaaaaaaa
      661 ggccgcgcca tgcgcagcca gaactcacaa ggaaaatgat agaaggaaat aacattagtg
      721 atgtacgcat ggcgcctccc gctaagttct gcaatgatca aacagaactc gctacgtgcc
      781 cttaaaactc gatcatttag cccctccaag gaggattcac catgcggttg gttttttaat
      841 aaacagtaaa caaaaaagtc aagaattatt cattctgttc tttcatcatc ggccacagca
      901 ataccacaat gccgcagacc agagcgccat cagtcagtac caacattatc ctgctggtga
      961 aatccatcat caccatcact aaaagcagga tcacaacagc aagcagacac agtttataaa
     1021 acaatgttca gaaaacgcat tcagcatgcc taaggttcta ttcctacgaa tagccaactt
     1081 gcaacttaaa atattattta tgcagccaat taaattctgg tccttacaat atcaacctga
     1141 agattcttat cttgtgctga ttgataaatg acaaaccttt tactacctgc attgaaagaa
     1201 gtagacaaaa ccagacaatt atcataacga gcaagaacat aataccaacc atcattataa
     1261 ttaatcattt catattcttt cttaaactgt ggtttgtaat atcctgtcag aaatgaaaaa
     1321 agccaaaaat atgccacaaa agcaatcatc acaatctcaa aaaaatgttt ttttataaat
     1381 ggcttatcat agaagcatga taccgataaa aatcgcccat aagatcttat cgaaattgta
     1441 accgccagcg caatcgctgc tgacagtagc aaaagaggta cctgaatctt ctgtctcaat
     1501 atagaaaact caataattgc cggcacaaac aataattcca cagcaaaata aaggcgaaat
     1561 acatttagct cttgcataga atgttttctt ttcactgcga aaaagaatac aacaccaata
     1621 ccccaaccga taagaaatat agcaatgacg ataactgcaa aaaataaact tctggcaaca
     1681 tcatcaacac ctgcacctac aatccaccat gggaagccgt agtaaaaaga agtaccccat
     1741 ccatagaaat aagcactccc ccatccaagg catcccatgt aggcaataaa aagtgaagaa
     1801 ctcctgagca gcgcaccatc cttcataacc accccaatac aagatgataa cattggctta
     1861 caactcataa caaaagcaat tcaatgccgt caagaggtta caggctaaaa aaactctatt
     1921 acattgcagt cagcatgttt actacacaaa tacaattcag agcataaaaa ctactcggcg
     1981 gcaggttatt gagactcatc aatgacatgt aaaaaacgcc cattattggt gtcaagtttc
     2041 cccaaagtta ttcaaaaagt caatattatg ccgttaatat gttgccatcc gtggcaatca
     2101 tggcgctaac gtgtgatcgc attcaaaatg ttgtctgcga ttgactcttc cttgtggcat
     2161 tgcacaacca gagcgtcata cagcggctta acagtgcgtg accaggtggg ttgggtaagg
     2221 tttgggatta gcatcgtcac agcgcgatat gcggcgcttg ctggcattct tgaataaccg
     2281 acgcctttac atcttccgca ctctttctca gcaactatcc cccactgctc tgttttggct
     2341 atatcaaccg cacggcctgt accgtggcaa tctctgcatc ttgcgcccgg cgtagcggca
     2401 ctacggcaat aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta
     2461 tttccttcaa gctttgccac gccacggtat ttccccgata ccttgtgtgc aaattgcatc
     2521 agatagttga tagccttttg tttgtcgttc tggctgagtt cgtgcttacc acagaataca
     2581 gccattccga atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg
     2641 gaaagagagt cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa
     2701 tgaaatttag ctacgctttc gagtctcatg cgccttctcc ctgtacctga atcaatgtga
     2761 ggtttccgca gaacactgcg ccagtatcga tatacatctg gttggcaaat ttgagtggtt
     2821 tcactgcttg cgtatgacca aagatgaacg tgtccgcgcc tttgatttct ttcacgatcc
     2881 cgtcttgtga gttgctgatt cgttcgcggt tccagattac ctgctgatga tcaactggct
     2941 ttccaaactc gtatttatca caaggataat cggcgtggca gatgacatat ttttttcctt
     3001 tactcaccag ttcgatgatt aacggaagtt catctgcttt atgggcaaga gctttagcta
     3061 gaatttcttt gtcgtaatcg agattaaaga accagccacc gccattaagc agccagtgat
     3121 tgacgtttcc acgctctgat aagccatcaa tcatcatttg ctcatggttt ccacgtacag
     3181 ctctgaacca ggggaatgtg attaattcca ggcattctac gttctctgta ccgcgatcaa
     3241 ccaaatcgcc caccgagata agcaggtctt ttttggtgtc gaatcctatc gtctccagtt
     3301 ttttcatcag gttcgtgtag catccatgca gatcgccagc tacccaaata tttcggtatt
     3361 tgctgccatc aattctttcg tagatattca tgcaacctca cttctgctgt ttcgcagttt
     3421 tttaagtttc tgttgatact ccgccttgat ggccctgcac tcttcgacag tccagcgata
     3481 gcggttatgg tttgattcga tttcctctac tgcttcctgc ccgatgcggc taatcagttc
     3541 gacgcgatac ggaacgagat ttccgctttt gtgctggttg cacaccacgc attgcttgtg
     3601 aatattgcgt tcatcaaatc ggagttgagg tgccgcagca gttgtccggt aatgtccggc
     3661 atcccactga gcagacgtga gtgttccgca cgagatacat ggtaagtcgc ggtctctttc
     3721 tctgatgaag gcgtttacgg cttgttgggc ttgtttaatc
//
LOCUS       DAZFYC010000245         3710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFYC010000245 DAZFYC010000000
VERSION     DAZFYC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.251"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..264
                     /locus_tag="ACHKW5_005183"
     CDS             <1..264
                     /locus_tag="ACHKW5_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7514350.1"
                     /translation="TEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAAL
                     RISISRWQLDLSALLASLQEEDGAATNKKTQADYARALSGEDE"
     gene            261..467
                     /locus_tag="ACHKW5_005184"
     CDS             261..467
                     /locus_tag="ACHKW5_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO7514351.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            464..2065
                     /locus_tag="ACHKW5_005185"
     CDS             464..2065
                     /locus_tag="ACHKW5_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7514352.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFVQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            2046..3365
                     /locus_tag="ACHKW5_005186"
     CDS             2046..3365
                     /locus_tag="ACHKW5_005186"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7514353.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            3375..3707
                     /locus_tag="ACHKW5_005187"
     CDS             3375..3707
                     /locus_tag="ACHKW5_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7514354.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
BASE COUNT          821 a         1009 c         1177 g          703 t
ORIGIN      
        1 accgaagcgc agcagctgac ggctgaagag caggtcgaaa aatgggtgga tggcaggaaa
       61 aaaatactgt gggacagcaa aaagcgacgc aatgaggcac tcgactgctt cgtttatgcg
      121 ctggcggcgc tgcgcatcag tatttcccgc tggcagctgg atctcagtgc gctgctggcg
      181 agcctgcagg aagaggatgg tgcagcaacc aacaagaaaa cacaggcaga ttacgcccgt
      241 gccttatccg gagaggatga atgacgcgac aggaagaact tgccgctgcc cgtgcggcac
      301 tgcatgacct gatgacaggt aaacgggtgg caacagtaca gaaagacgga cgaagggtgg
      361 agtttacggc cacttccgtg tctgacctga aaaaatatat tgcagagctg gaagtgcaga
      421 ccggcatgac acagcgacgc aggggacctg caggatttta tgtatgaaaa cgcccaccat
      481 tcccaccctt ctggggccgg acggcatgac atcgctgcgc gaatatgccg gttatcacgg
      541 cggtggcagc ggatttggtg ggcagttgcg gtcgtggaac ccaccgagtg aaagtgtgga
      601 tgcagccctg ttgcccaact ttacccgtgg caatgcccgc gcagacgatc tggtacgcaa
      661 taacggctat gccgccaacg ccatccagct gcatcaggat catatcgtcg ggtctttttt
      721 ccggctcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
      781 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaggatgact gctgctgcat
      841 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
      901 ctttaacggt gaactgttcg ttcaggccac ctgggatacc agttcgtcgc ggcttttccg
      961 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccggcgacag
     1021 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     1081 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgtgagtt
     1141 acccggcggg cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg
     1201 cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa
     1261 cacgcagctg cagagcgcca ttgtgaaggc gatgtatgcc gccaccattg agagtgagct
     1321 ggatacgcag tcagcgatgg attttattct gggcgcgaac agtcaggagc agcgggaaag
     1381 gctgaccggc tggattggtg aaattgccgc gtattacgcc gcagcgccgg tccggctggg
     1441 aggcgcaaaa gtaccgcacc tgatgccggg tgactcactg aacctgcaga cggctcagga
     1501 tacggataac ggctactccg tgtttgagca gtcactgctg cggtatatcg ctgccgggct
     1561 gggtgtctcg tatgagcagc tttcccggaa ttacgcccag atgagctact ccacggcacg
     1621 ggccagtgcg aacgagtcgt gggcgtactt tatggggcgg cgaaaattcg tcgcatcccg
     1681 tcaggcgagc cagatgtttc tgtgctggct ggaagaggcc atcgttcgcc gcgtggtgac
     1741 gttaccttca aaagcgcgct tcagttttca ggaagcccgc agtgcctggg ggaactgcga
     1801 ctggataggc tccggtcgta tggccatcga tggtctgaaa gaagttcagg aagcggtgat
     1861 gctgatagaa gccggactga gtacctacga gaaagagtgc gcaaaacgcg gtgacgacta
     1921 tcaggaaatt tttgtccagc aggtccgtga aacgatggag cgccgtgcag ccggtcttaa
     1981 accgcccgcc tgggcggctg cagcatttga atccgggctg cgacaatcaa cagaggagga
     2041 gaagagtgac agcagagctg cgtaatctcc cgcatattgc cagcatggcc tttaatgagc
     2101 cgctgatgct tgaacccgcc tatgcgcggg ttttcttttg tgcgcttgca ggccagcttg
     2161 ggatcagccg cctgacggat gcggtgtccg gcgacagcct gactgcccag gaggcactcg
     2221 cgacgctggc attatccggt gatgatgacg gaccacgaca ggcccgcagt tatcaggtca
     2281 tgaacggcat cgccgtgctg ccggtgtccg gcacgctggt cagccggacg cgggcgctgc
     2341 agccgtactc ggggatgacc ggttacaacg gcattatcgc ccgtctgcaa caggctgcca
     2401 gcgatccgat ggtggacggc attctgctcg atatggacac gcccggcggg atggtggcgg
     2461 gggcatttga ctgcgctgac atcatcgccc gtgtgcgtga cataaaaccg gtatgggcgc
     2521 ttgccaacga catgaactgc agtgcaggtc agttgcttgc cagtgccgcc tcccggcgtc
     2581 tggtcacgca gaccgcccgg acaggctcca tcggcgtcat gatggctcac agtaattacg
     2641 gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat ttacagcggc agccataagg
     2701 tggatggcaa cccctacagc catcttccgg atgacgtccg ggagacactg cagtcccgga
     2761 tggacgcaac ccgccggatg tttgcgcaga aggtgtcggc atataccggc ctgtccgtgc
     2821 aggctgtgct ggataccgag gctgcagtgt acagcggtca ggaggccatt gatgccggac
     2881 tggctgatga acttgttaac agcaccgatg cgatcaccgt catgcgtgat gcactggatg
     2941 cacgtaaatc ccgtctctca ggagggcgaa tgaccaaaga gactcaatca acaactgttt
     3001 cagccactgc ttcgcaggct gacgttactg acgtggtgcc agcgacggag ggcgagaacg
     3061 ccagcgcggc gcagccggac gtgaacgcgc agatcaccgc agcggttgcg gcagaaaaca
     3121 gccgcattat ggggatcctc aactgtgagg aggctcacgg acgcgaagaa caggcacgcg
     3181 tgctggcaga aacccccggt atgaccgtga aaacggcccg ccgcattctg gccgcagcac
     3241 cacagagtgc acaggcgcgc agtgacactg cgctggatcg tctgatgcag ggggcaccgg
     3301 caccgctggc tgcaggtaac ccggcatctg atgccgttaa cgatttgctg aacacaccag
     3361 tgtaagggat gtttatgacg agcaaagaaa cctttaccca ttaccagccg cagggcaaca
     3421 gtgacccggc tcataccgca accgcgcccg gcggattgag tgcgaaagcg cctgcaatga
     3481 ccccgctgat gctggacacc tccagccgta agctggttgc gtgggatggc accaccgacg
     3541 gtgctgccgt tggcattctt gcggttgctg ctgaccagac cagcaccacg ctgacgttct
     3601 acaagtccgg cacgttccgt tatgaggatg tgctctggcc ggaggctgcc agcgacgaga
     3661 cgaaaaaacg gaccgcgttt gccggaacgg caatcagcat cgtttaactt
//
LOCUS       DAZFYC010000246         3693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFYC010000246 DAZFYC010000000
VERSION     DAZFYC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.252"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..2106
                     /locus_tag="ACHKW5_005188"
     CDS             109..2106
                     /locus_tag="ACHKW5_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO7514355.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2169..3446
                     /locus_tag="ACHKW5_005189"
     CDS             2169..3446
                     /locus_tag="ACHKW5_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFO7514356.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          892 a          840 c          931 g         1030 t
ORIGIN      
        1 attccaccat gcattacgat taacgccgca tagccagttg aactttgcta ctttgtgaga
       61 ggtagtacct tctatccagt gcgaatttaa ttaatggaat aaatgattat gagtgaaaat
      121 gatacaatcc caaagaagtc tacaagtcag attaacaaag cggtattctt tacatctgct
      181 ttgctaattt tccttcttgt cgcctttgcc gccgtattcc cggatgtcgc cgacaaaaat
      241 tttaaactac ttcagcaaca aatcttcacg aatgccagct ggttctacat ccttgctgtg
      301 gccctgattt tactgagtgt cacgttcctt ggactctcac gctacggtga tatcaagctg
      361 ggcccggacc atgcgcagcc tgatttcagc taccactcct ggtttgcgat gcttttttcg
      421 gcagggatgg ggatcggcct gatgttcttt ggcgttgccg aacctgtaat gcattatctt
      481 tcgccacccg ttggcactcc agaaaccgtt gcggcagcta aggaagcaat gcgtctgacc
      541 tttttccact ggggactgca cgcatgggca atttatgcca ttgtggcgct gattctggcg
      601 ttcttcagtt accgtcacgg tctgccttta actctgcgct ccgcactcta tcccattatt
      661 ggcgatcgca tatacggacc tgtaggacat gcggttgata ttttcgctgt tataggcacg
      721 gtctttggcg ttgcgacatc actgggttac ggtgttttgc aggtgaatgc cggtttgaac
      781 catcttttcg gggtgcccat caatgaaacg gtgcaggtaa ttctgatcgt ggtcatcacg
      841 gggttagcga cgatttcagt ggtgtccggt ctggataagg gaatacgtat cctgtctgaa
      901 ctcaatctgg gtctggcttt gttgctcctg gcgctggtcc tgtgtctggg accaaccgtg
      961 cttctgctga agtcatttgt ggaaaatacg ggcggttatc tttcggaact ggtgagtaaa
     1021 acgttcaacc tttacgcgta tgagcccaag tcgagcaact ggctgggggg ctggacatta
     1081 ctgtactggg gatggtggct ttcatggtcg ccgtttgtgg ggatgttcat cgcacgggtc
     1141 tcccgcgggc gaaccattcg cgagtttgtc accggcgtgc tgtttgttcc cgcgggtttt
     1201 acgctaatgt ggatgacggt gtttggtaac agcgcgatct atctcattat gaaccagggg
     1261 gccacagacc tcgccaatac tgttcagcag gatgtggcgc tggccctgtt taatttcctg
     1321 gagcatttcc cgttctcttc tgtgctgtca ttcattgcaa tggcgatggt catcgtcttc
     1381 tttgtaacgt ctgctgattc gggggcaatg gttgtggata ctctggcatc aggtggagtg
     1441 gcaaacacac ccgtctggca gcgaatattc tgggcctcgc tcatgggcat tgttgcaatt
     1501 gcgcttctcc ttgccggagg gctaagtgcg ctgcaaacgg tgacaatagc gagtgcattg
     1561 cccttctcag tgatcttact aatatctata tacggacttt taaaagcttt gcgccgggat
     1621 ttgaccaagc gtgaaagcct gagcatggcg acaattgctc ctacggctgc acgtaaccca
     1681 attccttggc agagaaggtt acgcaatatc gcgtatctgc cgaagcgatc tcttgtgaaa
     1741 cgttttatgg acgacgttat ccagcctgcc atgacgctgg ttcaggagga actgaacaag
     1801 caggggacga taagccacat tagtgatgca gtcgacgatc gtattcgtct tgaagtcgat
     1861 ttgggcaacg agctgaattt tatatatgaa gtgaggcttc gcgggtatat ctcaccgacc
     1921 ttcgcgctcg ccgcaatgga taatgatgag cagcagactg aacaacatcg atattatcgc
     1981 gctgaggttt atctcaaaga aggcggtcaa aattatgatg tgatgggctg gaaccaggaa
     2041 cagctgatta atgacatact ggaccagtac gaaaaacacc tgcacttcct gcacctggtt
     2101 cgttaatagc aacatgccgt cctgggggcg gcaattatta tcccggccgc aatatgaggg
     2161 aatgcagaat gatttcacgc tggaaatgga tgctgaagca gacaattaaa aaactatggt
     2221 tcagggcaac gttattcgca attgtcgcga taataacggc ccttttatca attcttttta
     2281 aatcaatgat acctgagtcg gtttccgtga aggttggtgc ggaagcagtc gataacattc
     2341 tgaacatact ggcatcgagt atgctggcag tgaccacatt ttcgctgagt atcatggtca
     2401 cagcctacgg ttcagccact actaatgtga ctcccagagc tacgcgttta gttgttgaag
     2461 acgtcaccac acaaaatgta ctggccacct tcatcggttc ttttctcttc agtctggtag
     2521 ggattattgc cctcaatatg ggagcttatg gagaaagggg gagagtcatt ttattcattg
     2581 tcacactggt tgtcattgcc ttaatcctca tcacattgct tcgctggata cagcatttga
     2641 cctctctggg gagggttggt gagacaacgg cacaagtaga acaggcggcc atcgaaacat
     2701 ttattgcgag agcaagaaat ccctgtctcg gtggatatcc atggcttgag aacaatgaac
     2761 agccgaaagg aacggttgca gtttatccga agaagattgg ctatgttgaa tatattgata
     2821 tggtgaaact tagcaagctg ctgaccaatg atccccgtca tgtatacctc gtggcgcagc
     2881 caggcagttt catacatccg tccatgccag ttttgtacct gagtcagggc caggagtcgt
     2941 caatcaacac cgatttactt gagacgatta ttgtctcgga tgcacgttca tttgctcagg
     3001 atcctcgatt ttgtcttagc gtcatggccg aaatagcctg ccgggccctt tcccctgcag
     3061 tgaacgatcc tggaaccgcc attgatgtca ttggcagagg tgttcgtata ctttccactt
     3121 acgcgcagaa taaatctgat gaaatagaag tgaaatatcc ttcagtacat gttgcaccac
     3181 ttcagaataa cgatctactg gaagactttt tctcacctgt cgcgcgcgat ggtgccagta
     3241 tgagggagat tcagataaga gtccttaaag gactgtcgat gctgagtaaa ggttggcctg
     3301 agatatttgc tgaagccgca cataccctgg catttgaaac attagagcat gcaactcgtg
     3361 ctgaccatat agattctgat agatatctaa taaaatcaac ttattataat ttatttagtg
     3421 gcgaatattc taataaaaaa acatagctgc agagcaagca attcggttgt tggcaagtca
     3481 agggcaaagc attcgcgtga gaatgggcgt atgctttgtg ccaaaagcaa acgttgctaa
     3541 ttctgcactg ttcagcgatg gcgcagtaga gacaggcact gccactgtcc agtcggcaaa
     3601 agcgaacact ttgccactgc ggggataaac tgattgaagc tctcgcgcaa aatcacttat
     3661 tcttttgcgt taataaaatg taggagatgg gtc
//
LOCUS       DAZFYC010000247         3684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFYC010000247 DAZFYC010000000
VERSION     DAZFYC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.253"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..91)
                     /locus_tag="ACHKW5_005190"
     CDS             complement(<1..91)
                     /locus_tag="ACHKW5_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7514357.1"
                     /translation="MDTIDLGNNESLVYGVFPNLDGTFTAMTYT"
     gene            complement(257..664)
                     /locus_tag="ACHKW5_005191"
     CDS             complement(257..664)
                     /locus_tag="ACHKW5_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514358.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKALKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            748..978
                     /gene="dicC"
                     /locus_tag="ACHKW5_005192"
     CDS             748..978
                     /gene="dicC"
                     /locus_tag="ACHKW5_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HFO7514359.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            962..1483
                     /locus_tag="ACHKW5_005193"
     CDS             962..1483
                     /locus_tag="ACHKW5_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7514360.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKHAYRQQY
                     QQKAGWNYESSFC"
     gene            1464..2429
                     /locus_tag="ACHKW5_005194"
     CDS             1464..2429
                     /locus_tag="ACHKW5_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514361.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTGSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     ESELLDGGKVSESIRSKCAAPSGQNDTMEEAKMARSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTSRPEASQPDMQTAEQDFLNRHPDAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVSLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRVQRDPFWV
                     KNVMSPSKLREKWDELVIRLGRSPVQRCVKHISEPDTEIPPGFRG"
     gene            2436..3176
                     /locus_tag="ACHKW5_005195"
     CDS             2436..3176
                     /locus_tag="ACHKW5_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7514362.1"
                     /translation="MKNIATGGVLERIRKLAPQHVTAPYRTVDEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASLRSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            3202..>3684
                     /locus_tag="ACHKW5_005196"
     CDS             3202..>3684
                     /locus_tag="ACHKW5_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7514363.1"
                     /translation="METVLHALKAMGKANSVELAARIDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESRVTEERPVKSETQDMLTEEVAPKVSANMMIEFIGQDGAKTCEEI
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     F"
BASE COUNT         1002 a          765 c         1017 g          900 t
ORIGIN      
        1 tggtatacgt catcgcggta aacgtgccgt ccaggttggg gaatactccg tataccagag
       61 attcgttgtt gccaagatcg atagtatcca tgttgacctc atttcccctt aacgccgggt
      121 agcggaactg tttgctgaga acaccgtgcg gtgtcttgat gcaagcaaga ttagtcatgg
      181 ctaacaaatt ggtcaagcat ttttgtttgc catgactaac attttgggca accaaaaaga
      241 taacgcattg attgcgttat cttttgtttg ttcgttgacg ggcttttaat aactcttcaa
      301 agagtttgtt gaaatttttt actcgggcgc gcatctcggt gagctgagca tcctgttctg
      361 attcaggcaa tgcattaaaa agctcaagga gctcatgttc tttgggggat agagcaactg
      421 gctcctcaat aggtggtgat ggctgcttgt cttcatcgcc aaatagaatc catgttggcg
      481 agcactgcag tactttgctg agggcaaaaa gattctttcc tgtaggttcg ctatcatccc
      541 gctcccattg tgaaaccgat acgtgagaaa ttttcagggc tttagcaaga gacctttggg
      601 tgtatttgag gttttttcgg cgatatctaa tgcgttcgcc aatggttaaa ttttttgtat
      661 ccatagttag ctaatgctaa atcttattga ctatgttttt gttaacatct attttgttag
      721 tcatggctaa caattaaggt gctttaaatg cttaaaactg acgcactttt gtatttcggt
      781 tcaaaaacaa aacttgcaca agctgctggt attcgtttgg cttcgcttta tagctggaaa
      841 ggggagctag tacctgaagg tcgcgcgatg cgcctgcaag aggcatccgg cggggaactt
      901 cagtacgacc ccaaagttta tgacgaatat cgtaaggcaa agcgggcggg gcggttgaac
      961 aatgaaaatc acccctgaac aggtttgtga ggctctggat gcctgggtat gtcgaccagg
     1021 aatgacacag gagcaggcga cgatattaat cacggaagca ttctgggctc tgaaagaacg
     1081 cccgaacatc gatgttcaac gcgtcacgtt taatgatggc gaggttgatc aacgggcgct
     1141 gggcgttaac cgggtgaaga tattcgaacg ctggaaagct atcgacacca gggataagcg
     1201 ggaaaaattc acggcgctga ttccggcaat tatggaggct atccggatca gcgatttcag
     1261 attgtattgt gaaattactg acggaaaaag cattacgtac atgatcgccg ggttaaacaa
     1321 agaatatggc gatgtggtgg agtccgggct gctttttgcg gatccagctg ttgtggaacg
     1381 tgaaactgac gagcttatag aaaaagctat tgctttcaag catgcgtatc gtcagcaata
     1441 tcaacaaaaa gccggatgga attatgagtc ttctttttgc tgaacgccca ctggttatta
     1501 acactcagct ggcgatgaaa ataggtctga acgaagccat tgtgttacag cagctgcatt
     1561 actggttgag agataccggt tccggcatgg aatgtgatgg tgttcgctgg atttataaca
     1621 caacagaaca atggctggaa cagttcccgt tctggtcaga gtcaacgtta aaacgcgcat
     1681 ttgcaagtct gaaaacgctg gggcttttgc gttgcgaaaa gctcaacaaa tcaaagcgtg
     1741 atatgactaa tttttacacg attaattacg agagcgagct tttagatggt ggcaaagtga
     1801 gcgaatccat caggtcaaaa tgcgccgctc catcaggtca aaatgacacg atggaagagg
     1861 ccaaaatggc acgctccatt ggttcaaaac gacccaatgt catcgggtca aaatggcctg
     1921 atgatcttac agagaataca acagagatta ctacagagaa taaaaacact tctcgtccgg
     1981 aagcttcgca accggacatg cagacggctg aacaggattt tttaaaccga caccctgacg
     2041 cggttgtgtt cagtgcgaaa aaacgccagt ggggcagcca ggaggattta gcgtgtgcgc
     2101 agtggatctg gggacgaatc gtgagtcttt acgagcaggc tgccagcgat gatggcgaaa
     2161 tcatgcgacc gaaagaacca aactggactg catgggccaa tgatgtgcgc acaatgcgga
     2221 tgctggatgg cagaactcac agacaaattt gcgaaatgtt tggtcgggta cagcgggatc
     2281 cattctgggt aaaaaacgtc atgagcccgt caaagcttcg cgaaaaatgg gatgagctgg
     2341 ttattcgtct ggggcgttcg cctgtacagc gttgtgtaaa acatatttct gaaccggaca
     2401 ccgaaattcc gccgggattc agggggtaac gaagcatgaa aaatattgcg acaggcggtg
     2461 ttcttgaacg catccgtaag ctggctccgc agcatgtaac cgcgccatac cgaacagtgg
     2521 acgagtggcg cgagtggcaa cttgcagaag gccagaagcg ttgtgaggag atcaaccgcc
     2581 agaatcgtca gttgcgggtg gaaaaaattc tgaatcgctc cggcatccag ccgttgcacc
     2641 gcaagtgctc gtttgcgaat taccaggtgc agaatgacgg ccagcgatac gcgttgagcc
     2701 aggcgaaatc catcgccgat gaactgatga ccggatgcac aaatttcgca ttcagcggaa
     2761 aacctggtac cgggaaaaac cacttagcgg cggctatcgg gaatcgcctg ctgaaagatg
     2821 gccagacagt gattgtggtt accgtggctg atgttatgag tgctctacac gccagctatg
     2881 acgacgggca atcaggcgaa aaatttttgc gggaactgtg cgaagtggat ctgctggttc
     2941 ttgatgaaat tggcattcag cgcgagacga aaaacgagca ggtggtactg caccagattg
     3001 ttgaccgccg gacggcatca ctgcgcagtg tcgggatgct gacaaacctg aatcatgccg
     3061 caatgagcac gcttcttggt gagaggatta tggaccggat gaccatgaac ggtggtcgtt
     3121 gggtgaattt taactgggag agctggcggt caaacgttgg acgtcagggt atgtgagaat
     3181 ttttaacgag gtgaattttc gatggaaacc gtattacatg cactgaaagc gatgggaaaa
     3241 gccaattctg ttgaactggc ggcacgaatt gatatcagcc gtgaagaagt tctcaacgaa
     3301 ctgtgggagc tcaaaaaaaa tggcgttgtt gataaaacgg gtcacacctg gtttctggct
     3361 gtcgaaggtg aatcccgggt aaccgaagag cggccagtaa aatctgaaac acaggatatg
     3421 ctgaccgaag aggtcgctcc aaaagttagc gctaacatga tgattgagtt tatcggtcag
     3481 gatggggcta aaacctgtga agaaatagcg ggtaagttcg gtgtcagtac tcgcaaggtt
     3541 gcttccacgc tggcggtggt aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt
     3601 aaatttcgtt actgcatgcc gggcgataat ttaccagcag agccgaaagc cgcgctggta
     3661 acggaaagtg atggtaaggc cttt
//
LOCUS       DAZFYC010000248         3637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFYC010000248 DAZFYC010000000
VERSION     DAZFYC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.254"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(210..605)
                     /locus_tag="ACHKW5_005197"
     CDS             complement(210..605)
                     /locus_tag="ACHKW5_005197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514364.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELRYQKDNILQKLKAIRLDLETSPDHH"
     gene            781..1707
                     /locus_tag="ACHKW5_005198"
     CDS             781..1707
                     /locus_tag="ACHKW5_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFO7514365.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            1710..2273
                     /locus_tag="ACHKW5_005199"
     CDS             1710..2273
                     /locus_tag="ACHKW5_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514366.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLLKFIFFRAKMPFHTLA
                     LQDDLQTLLSPGTDPLFWSLICPGSFY"
     gene            2252..2569
                     /gene="sugE"
                     /locus_tag="ACHKW5_005200"
     CDS             2252..2569
                     /gene="sugE"
                     /locus_tag="ACHKW5_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7514367.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            complement(2566..2649)
                     /locus_tag="ACHKW5_005201"
                     /pseudo
     CDS             complement(2566..2649)
                     /locus_tag="ACHKW5_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          852 a          874 c         1027 g          884 t
ORIGIN      
        1 aaaaaaaaaa ggatctgcat catgtgagag tgatcccacc gctcgccgca gccccagcga
       61 ccgaacgtag tgagcgagtg ggcgaggaag cggaagtgaa ccggaaacca cattgagcac
      121 gtacgcacct atgccagatc cgcgcgtagc gcgtcgcgaa atcgaaaaca ttgaacaccg
      181 gcaggcaaac gcaccaccac gcagcggaat taatgatgat ctgggcttgt ttcaagatcg
      241 agcctgattg ccttaagttt ctgtaatata ttgtcttttt ggtatctcag ttcgcctgtt
      301 tttgtgtcca ctagtccggc ctcgatcagt aaccccgccg caagaaacag gcggtgattg
      361 cgttcccgtc gttgtgtttt cccctccatc cgtttttggt tggcctgttt gcgtgcgttc
      421 tcctgtgcct gtagctcgtt tttttctgcg gttgccagga cgtggagtag tttgcgctcc
      481 tgttctgtta gtccggcttc gttatcgagc aggtcaatga tctgcctctg tcgtgcggtc
      541 tgttctccga gcttcctgat aaagccgatt cgtttcttca accagtcgat gttgctgatg
      601 cgcatgtttg ggatgctccc tggtgttttt tgcggtgtgt tggggttgtg cagtatatcg
      661 cttgcggtcg gtgggtgcag ggggtatctt ccgtgctaat tcgtaaaatt agaaggaagc
      721 gcacttatat gtttctctcc ttcgtcgaga aacagtgcgc tcctgcggag ggctgaaacc
      781 gtggcaacct atcacctcag cgttaaattt ggtggcaagg ggcaggctgc aaaccacgcc
      841 gattacatcg agcgcaaaga gaaataccgc gaccgtcagg atttggaata ttccgcacac
      901 ggaaacatgc cggaatgggc gcgagataat ccctcacatt tctggcaggc ggctgaccag
      961 tttgaacgtg cgaacggttc gacctatcgg gaacttgaaa ttgcattacc ccgtgaactg
     1021 acagcggaac agcgccttga actggtgcag gattttgtac gtcaggaagc tggcgaacgg
     1081 cacgcctgga gttttgccat tcacaatccg aaagcgagca ttgacggcgg cgaacagcct
     1141 catgcgcata tcatgatgag ccagcgtgtg aatgatggca ttgaacgtac accggaacag
     1201 tatttccggc gctataacgc caggtatccc gagcggggag gtgcgaaaaa ggattccgga
     1261 agcctgactc tcactcagca aaaagagcag ttgcgggaat tgcgaaaacg ctgggaggtg
     1321 aagcacaacg aacatatgag aaagcatggc tttgaaaggg gcttcattga ctgccggacg
     1381 ctgaaagaac agggcattga acgacgtccc gaagtgcatc tgggttttga agctgccgga
     1441 cggcttgatg atgcccagcg tcatgacatc atgcaaaaac ggtctgaacc ggactattcc
     1501 cgccatcttg aacaggcgcg aacggtcggg catgacatca gggaatggct ggacactccg
     1561 gttaagcaac atgaactggt cagaaggctg caacatgatg ttgcgcggga gcatggcttt
     1621 gacatgagag acagagtgag cagacagaac cgaactgaac ctgaacgggt taaatcaccg
     1681 tcacgtggat tttctatggg attctgaaca tggccagcac agaagaaaaa ttactcacgc
     1741 tcctgttatc ggtggagcat ctgcaacata ccgcaatgga gcaacagaaa gctgttgctg
     1801 attctctggc tgtggcaagg gaaacacttc gcggatatcg ggatatttct gacggtgtgg
     1861 caaaacaggt cagggatgaa gtcagaaacg aggtgcagaa gctggatgtt gcagggctga
     1921 taggtgagcg catcagccag gattttcagc ggctggaaga gtcagtcgca aacatgaagg
     1981 caaacgcgga tgccctgaac agggatattg cgacaattca cagcaatatc gtgcaggaat
     2041 acaacaccct taaagggtat agctggaaat ggctgggtgg ggtgtcagtg gcttttctgg
     2101 ttatgttctg tgtgcttgga tggggaatga aaacaatgct cttgaaattc attttcttcc
     2161 gggcaaaaat gccatttcac acattagctt tgcaggacga cctgcaaacg cttctttcac
     2221 cggggacgga cccattgttc tggagcctga tatgtcctgg atcgttttat taattgcagg
     2281 tttgctcgaa gttgtctggg cgattggcct gaaatacacc cacggtttta cgcgtcttac
     2341 gccaagcatt atcactattg cggcgatgat cgtcagtatc gccatgctct cttgggcaat
     2401 gcgcacgttg cctgtaggaa ccgcttatgc ggtctggacc ggtattggcg ctgttggggc
     2461 ggccattaca gggattttgc tgctgggtga gtctgccagc ccggcacgtt tgctgagcct
     2521 tgggctgatc gttgctggca ttattggtct gaagctgagc actcactaag cgccaggctg
     2581 tttcacccaa atcagtttat tcacttcaaa cccttcccgg gtcgcgatgg ctaacatttg
     2641 ctgtttcagg aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga
     2701 taaactggcg gagaaaccag caacagggaa gagccggaaa aactgacgcg cttcgcttgt
     2761 ccaacgcctc acgggacgcg aaaaaattcc gcgtcccgtt caccgttttc tgatgagaaa
     2821 gtttgccgtt ttgacccgtt cggtgctcag atttggcgaa attgattccg aatcactttt
     2881 gtgaatagtt atgcaagccc ctggaaacgt ttctgacgcg ttttttgagg taggggtggg
     2941 ggaaagcatg gttttttgat gttttgagcg taaaagcgtg aaaaatcgcg ttattgtgct
     3001 tgcgatcttt cgtcagggag cgtagaacga aaaaagcccg ccgttaggcg agcttcttcg
     3061 ggttcattga ggttttcgac ctcctcaatg ttcctgtgga gtctgttgca gtgacaacgg
     3121 aaaccagttc attcagtttg catgaacctt ctgtattctt caagcattac cgaccgcaca
     3181 gcctccccag aatcccgaaa aaatcctgtt tttgcagatg attgcgtgtt tgcaaagacc
     3241 gtttgcggtt gggggagctg tgacgacggc aaccgcacgc gtgagagttc cgctaccggc
     3301 gcagagagtt tcatcgacag cggacacctg atgagttgcc acaaaaaccg tatgtagcgc
     3361 ggctagattg gttgagttag accttggcgg cagttcccgg tatgtggtgg cgtgacgggt
     3421 gagatggtgc ggcgaagacg catacgtcac gcaggcgcgg gttcttccac cgacgcgccc
     3481 cgtgatcgat ggccttggct ccgtccggca tatcgagagc ggcaaactac ccgtcccttc
     3541 actgggaggg gtgagttgtt gccgttccct ccgaccttcc accatccagc atatcgcacg
     3601 atcccatgag caccgcaaca actgataact ccagagc
//
LOCUS       DAZFYC010000249         3618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFYC010000249 DAZFYC010000000
VERSION     DAZFYC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.255"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..316)
                     /locus_tag="ACHKW5_005202"
     CDS             complement(20..316)
                     /locus_tag="ACHKW5_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO7514368.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            complement(455..655)
                     /locus_tag="ACHKW5_005203"
     CDS             complement(455..655)
                     /locus_tag="ACHKW5_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7514369.1"
                     /translation="MEQRITLKDYAIRFGQTKAAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEIKPFPSNKKTTA"
     gene            756..1469
                     /locus_tag="ACHKW5_005204"
     CDS             756..1469
                     /locus_tag="ACHKW5_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7514370.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            1479..1820
                     /locus_tag="ACHKW5_005205"
     CDS             1479..1820
                     /locus_tag="ACHKW5_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HFO7514371.1"
                     /translation="MAFTDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            1888..2349
                     /locus_tag="ACHKW5_005206"
     CDS             1888..2349
                     /locus_tag="ACHKW5_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514372.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTTETNEAVLAALSAEVRGC"
     gene            2343..3389
                     /locus_tag="ACHKW5_005207"
     CDS             2343..3389
                     /locus_tag="ACHKW5_005207"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HFO7514373.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQYAAQCESNIYIAMPFYHNGSLNQLMKKN
                     NLTSREIIRYSIQFLSGLYHIHSKGLMHFDIKPNNIMISNRNEAMLSDFGLSQLVNEE
                     SRAAPEFGYHFHVPPEYFSLSTNDYNFTYDIYQAGLTIYRMCVGHDNFERERSAFSTI
                     EQLRESIINGCYPLKEYPPHIHKKLITIVNKCIHVDPNERYQSVLDVLNDLSAISDGV
                     LDWRLQMTKPTNGTCEWQKKSGDAILSIVFDAENSSTTGFRLYDDGRKRRATNLTISS
                     GCTPTKLYRLLKDN"
     gene            3392..3556
                     /locus_tag="ACHKW5_005208"
     CDS             3392..3556
                     /locus_tag="ACHKW5_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514374.1"
                     /translation="MKKREEVSKLPRRRDAALAVPYKKDEFISPSDDKKFSKAKSFTS
                     TSLKDKYFKI"
BASE COUNT         1136 a          699 c          769 g         1014 t
ORIGIN      
        1 tattgatcca gtaattccct cagaattgca tatcaatttg cttagagtcc ccggcggcca
       61 ccggggattt tttctttgtg atttcatcaa gcgcatactt aaaagccctg ctaatcggac
      121 tgatgtctga tgccattccg aaagcacaca agaccgaagc aataaatctc cagtccgttc
      181 tgcttatctt cgattcatga cagccaatca tctttgccag accgcgctgg gtaagcgttg
      241 acaggttgat gagtaaatca gtttcagcgc gatcaatttc tcgctgtgat agtttgctgt
      301 aacttgtttg ttccatttct taagatttcc aatagtgaat agctagttga aaggtatgcg
      361 tggaaacgca tatggcctta gttggtcaga tatattggga ctcgctttgt cagcgacgta
      421 ggacgaatgt ccattgtgaa aagagcggtg ttacttatgc agttgttttt ttgttacttg
      481 ggaagggctt tatttcttcc gcataaacgc ttccatcagc gtttatagtt aaaaaaatct
      541 ttcggcctgc atgaatggcc ttgttaatcg cgctttgata tacgccgaga tctttagccg
      601 ccttggtttg cccaaagcgt attgcataat ctttcagggt tatgcgttgt tccatacaac
      661 ctccttagta caggcaatca ttatcaccgc tagaggtata atagtcaaca cgcacggtgt
      721 tagatattta tcccttgcgg tgatagattt aatgcatgag cgcaaaaaag aaaccgttaa
      781 cacaagagca gcttgaggac gcacgtcgcc ttaaagctat ttatgaaaaa aagaaaaatg
      841 agcttggctt atcccaagaa tctgtcgcag acaagatggg gatggggcag tcaggtgttg
      901 gtgctttatt taatggcatc aatgcattaa atgcttataa cgcagcattg cttgcaaaaa
      961 ttctcaacgt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc tacgagatgt
     1021 atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct gttttttctc
     1081 atgttcaggc cgggatgttc tcgcctgagc ttagaacctt taccaaaggt gatgcggaga
     1141 gatgggtaag cacaaccaaa aaagccagtg attctgcatt ttggcttgag gttgaaggta
     1201 actcaatgac cgcaccaaca ggttccaaac ccagttttcc tgacgggatg ttaattcttg
     1261 ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt gggggtgatg
     1321 agtttacctt caagaaactg atcagggata gcggtcaggt gtttctacag ccactaaacc
     1381 cacaataccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa gttatcgcca
     1441 gccagtggcc tgaagagacg tttgggtgag gaggatagat ggcgttcact gaccttgaat
     1501 atcaagcggt caaaaaagaa gttcaccaat tcattgaaag cataaggccg cctgaacata
     1561 tccgcaatga actggatatt gtttatagca tcaatgacca aacgatagat atcggcgaac
     1621 agcgccccgt gtggcagggc aacccaggtg aaacaaacat cctgccatca gcaagaatca
     1681 agtacatacg ttctctggat agatggaaaa tctattggat gcggaaggat atgaaatggc
     1741 atcagtacag tactgaactt tcgctgactg atgcgcttga gcttgtgcgt gctgacccgg
     1801 attgctgctt cttcggatga gtgaagagac gtttggatga tggatggtcg cagagatgcg
     1861 gcctgattct aaaataggat ataaaaaatg agaatactag gtgttagagc ggcgcccaaa
     1921 gttacatctt ttgttgtata ttgcactaat gagtctgcac tcaaatgtgt tgatgtcatt
     1981 aaaatacctt cgaccttaga cacaccagaa aaattaaagt atgtgagaaa taacatcctc
     2041 gacattctta atttatataa tgttgaatta gctgccatac gcgttactga atcaaactct
     2101 gataatctta gcattgaccg cctttatata gaagctgtta ttcaagaagc attttcaagc
     2161 agtgatgtaa gaaaatatta cactattaga aaatctggca tgaaatcatc attgaaccta
     2221 acagagatcg agtataaaga aatattgaag tcacaccgca atataaatgg aatcgataat
     2281 tctggtttta caactgaaac aaatgaagct gttttggctg cactatctgc ggaggtaagg
     2341 ggatgctaac tccatacaaa agagctgatg tagaattcga atggattagt gatctagaag
     2401 aacagggttg tttttcaaaa gtatatctgg ctcatgacag acacctagct catgacttgg
     2461 tgattaaaga aatagaaaaa aaagaaaaca ctaaccacga cgactacttt aatgaagcaa
     2521 ggcttctcta taaacatgca catccaaata ttgtgcaagt tcagtatgct gctcaatgtg
     2581 agagcaatat ctatatagcc atgccatttt atcataatgg ttcgctaaac caattaatga
     2641 aaaaaaataa tcttacaagc agggagataa tacggtattc cattcaattt ttaagtggac
     2701 tttatcatat acactcaaaa ggtcttatgc attttgatat aaaacctaat aacattatga
     2761 tatcaaacag aaatgaggcc atgctatctg actttggatt atctcagtta gtcaatgagg
     2821 aatcgagagc tgcgcctgag tttggatatc attttcatgt gccaccggaa tatttttctt
     2881 tatcaacaaa tgattataat ttcacatatg acatatatca ggcaggatta accatatata
     2941 gaatgtgtgt tggacatgat aattttgaaa gagaaagatc tgcatttagc acgattgaac
     3001 aactcagaga gtcgataatt aatggctgct atccattaaa agagtatcct ccccatatac
     3061 ataaaaaatt aataacaata gtgaacaaat gcattcatgt agatccaaat gaaagatatc
     3121 aatccgtact agatgtacta aacgatctct cagctataag tgatggcgtt cttgactggc
     3181 gtctacagat gacgaaacca actaacggca catgcgaatg gcaaaaaaag tctggggacg
     3241 ctatactgtc tatagttttt gacgcagaaa attcgtctac tactggtttt cgtttatacg
     3301 atgatgggcg gaaaaggcgt gctacgaact taacaatatc ctcaggatgt acccctacaa
     3361 aactgtatag gttattaaag gataactgat catgaaaaag cgcgaggaag taagcaagct
     3421 gcctcgcaga cgtgatgcag cattagcggt tccctacaaa aaagatgagt tcataagccc
     3481 ttctgatgac aaaaaatttt caaaggcgaa aagttttaca tctacatctc taaaagataa
     3541 atactttaaa atctagcccg gcctcagcgc cgggttttct ttgcctcgcg atcccccacc
     3601 taaaaaaaca taactcat
//
LOCUS       DAZFYC010000250         3600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFYC010000250 DAZFYC010000000
VERSION     DAZFYC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.256"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(111..446)
                     /locus_tag="ACHKW5_005209"
                     /pseudo
     CDS             complement(111..446)
                     /locus_tag="ACHKW5_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(451..666)
                     /locus_tag="ACHKW5_005210"
     CDS             complement(451..666)
                     /locus_tag="ACHKW5_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7514375.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDKVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(742..1011)
                     /locus_tag="ACHKW5_005211"
     CDS             complement(742..1011)
                     /locus_tag="ACHKW5_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7514376.1"
                     /translation="MSEIKSLVTAEAVKDVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGGPAAPEPEDGAGDSAVSDGVVSQPDGSSEPQPDGEMMM"
     gene            complement(1049..1231)
                     /locus_tag="ACHKW5_005212"
     CDS             complement(1049..1231)
                     /locus_tag="ACHKW5_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7514377.1"
                     /translation="MRFVQLILLYFCTVVCTLYLVSGGYKVIRNYIRKKIDAAAAEKI
                     SASQSAGTKPEEPLIS"
     gene            complement(1379..3316)
                     /locus_tag="ACHKW5_005213"
     CDS             complement(1379..3316)
                     /locus_tag="ACHKW5_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7514378.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVPGTGDGGYPGVVTTEPDYYYVIPLAGQSNGMAYGEGLPLPQTY
                     DRPDPRIKQLARRSTVTPDGAPCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDVTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFDGTPANHTARFTEVVEQYRTDLAD
                     MVGQCAGGSADGVPWICGDTTYFWKQKSESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     ANVPTNKPEEDPDIPASGYYGAASRTSANWTSADRASHFSSWARRGIISDRLASAILL
                     HAGRTAELVGGEQVVMPPDEKPSPDTPSTPSTDGKSVTTLLYYRATESGGLLNPQGWG
                     AEGGRALVVDDAGAAGGKALRWTKQTGSSSWFMQHDAGNGADLLEKGGLISCRFKVDG
                     TLTANQYALALYWPVSSLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNAGQNT
                     KLGSFGAFDNEWHTLGFRFAGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTD
                     ITSGATYPVLIESITVEVNNP"
     gene            3293..3436
                     /locus_tag="ACHKW5_005214"
                     /pseudo
     CDS             3293..3436
                     /locus_tag="ACHKW5_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3482..>3600
                     /locus_tag="ACHKW5_005215"
     CDS             3482..>3600
                     /locus_tag="ACHKW5_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514379.1"
                     /translation="MAKHGAGCLPVNSVPAPESAIIPYTWLLIAPPHRGIHHAV"
BASE COUNT          817 a         1055 c          822 g          906 t
ORIGIN      
        1 gctgcaagca cctcattttt tgttggggtg ctttttttca attcaataac acagcactcc
       61 agtttttcaa tacgtgattc aacatcatct ttttctgacc gcagtgttga cggcggcatc
      121 ttcagagaac aagtaattct tcccggtagc tttcctttgt aggttatcaa cacatcctgc
      181 gcctctaaaa ttacggggcg attttccggc aacggaccat ccccttcaca taacccggca
      241 gcaacatcca tgaaaaactg cttcgcctgc tttttcgcct cagcttcgta aaactccagc
      301 gtggcacctt cagtacggtc aagactaatc gccacatctg gcaacaacag tgacggatac
      361 ccaccaattt ccagtgccac agtaacagta atcttatccg ggtaattatt tatcccttta
      421 acaaccagtt cgtatttttt cttcatcgct ttactctccc cgcgccgcct tacgacggtc
      481 ctctctgatt ttgaaataca ggttagtcag atatgtcagc agcccaaaca gcagactccc
      541 cagcacgcct attgccgccc actgagacgg agagactttg tccagcagct gcaggaacca
      601 gtagcccgtt cccaccgctg acgtggtgta tgacacacct gttgtgattt tttccatctg
      661 gtacataccc cgtctctcgc aatccggaag ctcacaacaa caggaggggc atcagctcac
      721 accgacagcc cctgcgcatg gttacatcat catttcgccg tcaggctgag gctcactgct
      781 accgtcaggc tgagacacga cgccatctga aacagcactg tcacccgcgc cgtcttcagg
      841 ctcaggagca gccggtcccc ccagcagctc atccagaatg gcatccactt cagcatcaag
      901 acgcgcctca agattctggc ggagttgctg tttcagtgcg cttctgactt cttcagagcg
      961 caggacgtcc ttcactgctt cagcagtgac cagagatttt atttctgaca tgggattttc
     1021 tcgttgaaag gtgttgttaa gaaagttgct acgaaatgag aggctcttcg ggttttgttc
     1081 cggctgactg gctggcgctg attttttccg ccgccgcggc atcaatcttt ttgcgtatat
     1141 agttccggat aaccttatac ccgccactta ccagatataa cgtgcacacc accgtgcaga
     1201 aatacaataa aataagctgt acaaatctca ttatccctcc cggttattga tatggtgttg
     1261 acatcgttaa tacctgttgg ttaaaaaagt gtcctgcatg ttttgctttg gatataacga
     1321 catttgccgc cggttctggc tccttgtttt ccctgccccg gcggcctttt tttcctgctt
     1381 acgggttatt cacttccact gttatacttt caatcagcac cggatatgtc gcaccgctag
     1441 tgatatcggt cacgcgcaat ttgtctgccg taaacgtgcc gaccggtgac tgtgacagca
     1501 tgaacggcgt cccgtcctta ccatcaatga ccggcgtcac ctcaatactg ttgttaccgg
     1561 caaaacggaa gcccagcgta tgccattcgt tatcaaatgc gccgaatgac cccagcttcg
     1621 tgttctgacc agcatttccc ttgtggtaca tcacattaag gtctgtggca tcgctctgta
     1681 cgtaaaacga cgccagcagg ttatgaccgg cattaccttc cagtgtgacg ccctgaggca
     1741 gtgaagaaac cggccagtac agcgccagtg cgtactgatt agctgtcagt gtgccatcaa
     1801 ctttaaaacg acaactgata agcccgccct tctccagcag gtctgcgcca ttaccggcat
     1861 catgctgcat aaaccacgag gaacttcctg tctgtttggt ccacctcagc gccttacctc
     1921 ctgcagcacc tgcatcatca actaccaatg cacgccctcc ttcagctccc catccctgcg
     1981 gattcagtaa accacctgac tctgttgcac ggtaataaag cagcgttgtc actgatttcc
     2041 cgtccgttga cggtgttgat ggtgtgtccg gtgacggctt ctcatccggc ggcatcacaa
     2101 cctgttcccc acccaccagt tcagccgtcc gtcctgcatg gagaagaatc gctgaggcaa
     2161 gacggtcaga aataatcccc ctgcgtgccc atgagctgaa atggctcgca cggtctgctg
     2221 acgtccagtt tgccgacgtc cgggaggccg caccgtaata tcctgatgcc ggaatatccg
     2281 ggtcttcttc cggtttgttc gtcgggacat ttgctccgtt ctcatcggtc atgaacggca
     2341 caaagtgaat attcttttcc gttttgtttt tgtaactgcc gtacaccgtc tggtaagtgg
     2401 attcgctctt ctgcttccag aaataagttg tgtctccaca tatccaggga acaccgtcag
     2461 cagaaccacc agcgcactgt cccaccatat ctgcaaggtc cgtacgatat tgttccacta
     2521 cttctgtaaa acgggctgtg tgatttgctg gcgttccgtc aaagtcaaat tccccctgca
     2581 tccacaccac ggcaagcagc acatttttcg ggttctttgc cagcgcggct tttgtacgac
     2641 cgatgagatc cttatacagc ggcctgccca caccccagcg ggtagaactc tctgaggcac
     2701 cggtcacgtc actgtatgtt ccatctgccc cggtggtgaa agctgaacca ccacggcagc
     2761 acggaaccag aagaataccc gcattcgccg gtataaacgg cagcagcttt ttggcaatat
     2821 gcagcccctg ccccacggtt ccgtactgac ctttcgacag gtcagctttc ggatggttaa
     2881 gacggctcat gtcctgtaca tcatgcagac agtggtctgc cggaataatg tcgttatatt
     2941 tacagggagc gccatccggc gtcactgtgc tgcgacgagc cagttgcttt atacgcgggt
     3001 caggacggtc atatgtctgc ggcagaggaa gcccctcacc gtaagccatg ccgttcgact
     3061 gcccggccag tggaataacg tagtaataat ctggctccgt ggtgaccact cccggatagc
     3121 caccatcccc cgtgccgggc acaaccactg gcgtggtcac atccccctcc gcggcaatcg
     3181 cctgcatcag ggtataaggg gttatggcca ccggactacc aaacggctgc cagccctctt
     3241 tcagtttgtg tgtcagcttt tccgcaagat ctgacggcga cgccgccctg acaacatcat
     3301 aatgtttaat cgacatcgaa tttctcccgt gtacaggaac agagttaaaa agccggaacc
     3361 ggaatcaaat cacaggatga ccatctgcca gtggctggtc gtaaaaaaaa ggccgcgcca
     3421 tgcgcagccg gaaataaagg gataacgatg atagtttgag aaaaacagaa ataacacttt
     3481 tgtggcaaag catggtgccg ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc
     3541 gattatccca tatacctggt tgctgattgc ccctccgcac agggggattc accatgcagt
//
LOCUS       DAZFYC010000251         3533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFYC010000251 DAZFYC010000000
VERSION     DAZFYC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.257"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            155..523
                     /locus_tag="ACHKW5_005216"
     CDS             155..523
                     /locus_tag="ACHKW5_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7514380.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            613..987
                     /locus_tag="ACHKW5_005217"
     CDS             613..987
                     /locus_tag="ACHKW5_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7514381.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            984..1472
                     /locus_tag="ACHKW5_005218"
     CDS             984..1472
                     /locus_tag="ACHKW5_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7514382.1"
                     /translation="MKLSLMLEANRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            1484..1681
                     /locus_tag="ACHKW5_005219"
     CDS             1484..1681
                     /locus_tag="ACHKW5_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7514383.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            1778..2347
                     /locus_tag="ACHKW5_005220"
     CDS             1778..2347
                     /locus_tag="ACHKW5_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7514384.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRYRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(3214..>3533)
                     /locus_tag="ACHKW5_005221"
     CDS             complement(3214..>3533)
                     /locus_tag="ACHKW5_005221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514385.1"
                     /translation="VHISKVISAGDKIMPLSIGSCFSCHTGRRPEISDPIIDKPSTKN
                     CELISTTCNVDGITVISRSSYGFDMKPPGAGERAPRLKLSASEAQWMAAIIDAEGHNI
                     SNT"
BASE COUNT          941 a          910 c          776 g          906 t
ORIGIN      
        1 tcacccggaa acgagtacga ccagatggac tcagacgccg ctgtggatga ggccattctc
       61 gtcaatgcat acatcttcac cgaaaccggt cagcgcgccg gctgacctgt catcgttaaa
      121 ccattcttaa ccacttttca gagaggattt tatcgtgtca gacaaactct ccgggataac
      181 tcatcccgac gacaatcacg accgcccctg gtgggggctt ccctgcacag tgaggccctg
      241 tttcggggca cgtctggtgc aggagggtaa ccgcctgcat taccttgccg accgcgccgg
      301 tatcagaggc ctgttcagcg atgcagatgc gtaccacctg gaccaggcct ttccgctgct
      361 gatgaaacaa ctggaactca tgctcaccag cggtgaactg aatccccgcc atcagcatac
      421 cgtcacgctg tatgcgaaag gtctgacctg caaagctgac accctcggta gcggtggtta
      481 cgtttatctg gctgtttatc cgacgcccga aacgaaaaag taaaccaccg gaataacctt
      541 ctgctccggc ctggtgcttt caccacgcca cttttccatt tttcatctct gcatatcagg
      601 aaaaccttca gtatgaaaac attacccgat acacacgtac gggaggcatc gagctgcccg
      661 tctcccgtca ccatctggca gactctgctc tcccgactac tgggccagca ttacggcctt
      721 acactgaatg acacaccgtt cgccgatgaa cgtgtgattg agcagcatat tgaagctggc
      781 atttcactgt gtgatgcggt gaattttctc gtggaaaaat acgcgctggt gcgtaccgac
      841 cagccgggat tcagcacttg tccccgctct cagttaataa acagtattga tatcctccgg
      901 gctcgccggg cgaccggcct gatgacccgc gacaattaca gaacggtaaa taacattacc
      961 ctgggtaagc atccggagaa acgatgaaac tttccctgat gctggaagcc aacagaatta
     1021 atgtgcaggc actgaacatg gggcgaattg tcgttgacgt cgatggtgtt aatctcactg
     1081 aactgattaa caaggtcgct gaaaacggtt attcactccg cgtggtggag gaatccgacc
     1141 aacagtcaac ctgcacacta ccaccgtttg caacccttgc cggcatacgc tgcagtaccg
     1201 cacatatcac ggaaaaggat aacgcctggc tgtactcgct gtcacaccag accagtgact
     1261 tcggtgaatc agaatggatt catttcacag gtagcggata tctgttacgt accgatgcgt
     1321 ggtcatatcc ggttctgcgg cttaaacgcc tggggctgtc aaaaacgttc cgtcgtctgg
     1381 ttatcacact tacccgacgt tatggcgtca gtctcattca tctggatgcc agcgctgaat
     1441 gcctgccggg tttacccact ttcaactggt aaccaggaac aacatgaaat cattaaccac
     1501 ggaaaccgca ctggatattc tgattgcgtg gctgcaggac aatatcgact gcgaatcggg
     1561 aattatcttt gacaacaatg aggataaaac ggattcagca gcactgttgc cctgtatcga
     1621 acaggccaga gaggatatcc gtaccctgcg ccaactgcag cttcagcacc agaaccggtg
     1681 agtctcactc atcatctcac tcaccagact tcattccact gacgccagcc tgaacacggc
     1741 tggcgttttc atttatctgc aaaaaggaat atcgattatg tctgaaatca cagtctcccg
     1801 tccggaagtg gtcaacgaga atacggacgt tatctgctcc acctcagtca ggtacaggtc
     1861 actggaatat gataattttc cggaaatcag cgaagcgaac attctgagca catttgaaca
     1921 actgcaccag aacaaagatg aagtgtttga acggggagtg atcaacgtct tcaaagggct
     1981 gagctgggat tacaaaacca actcaccctg taaatttggc agtaaaatta tcgtcaacaa
     2041 tctggtgaga tgggaccagt ggggatttca tcttatcagt ggaatgcagg cagatcgcct
     2101 ggctgacctg gaaagaatgt tgcatctgct cagcggtaaa ccgatccccg acaaccgagg
     2161 gaatatcacc attaatctgg atgaccacat acagtccgtt cagggtaaag gacgctatga
     2221 agatgagatg ttcatcatta aatactttaa gaagggatct gcacacatca ctttcaaaag
     2281 gctggagctg attgacagaa ttaacgatat aatagccagg cactttcctt ctgtgctctc
     2341 agcctgaccc cgagtttgat tccctttcga tatcaaaagg gactgcgggt acaaaagagg
     2401 gtacatattt caccaaacca aacaaaataa actaatatca acatgataga agcattcttc
     2461 gattccgagt ccggcaccaa attcataagg cctcgctgaa aagcggggct ttttgctttt
     2521 ctggctacgg tagatttctg gtcgttgcaa tacgttattt gtttgttgta tttctcacta
     2581 ttttcgccga tgttcgttac ttttagacac cccaacatcc tatgatgtgt gccttgtcct
     2641 tactctacta ctacggtctt tctacggttc ccttttctca gaacattcat aatgaagtag
     2701 aaaacacggg gaaacaggag agttatcatt tcgccttaat ccgcaggttt taacgtgatt
     2761 ttgtcgcgga gaataaggtg ggaaaatctt caataacaag cgggaaaacc tgatacgcca
     2821 ttgacatggg gctcttcacc actccgtcca cctgcaacag actcttcttt ccttctcgct
     2881 tcttaccctt accagaatcc tctgtcctcc acgagatcat aaaatgatgt atcagaagta
     2941 atacgacttg aacagtacaa aatagtggta catttggaat cgaagtgtaa aggtaactta
     3001 ctgttatttg gtgatttcat tgccgtgtca atgaatcctt aattcgattc cgaatcaggc
     3061 accagactca tatcaacgga cctccatgga ggtccgtttt tcatccataa tcgccttcat
     3121 ttgatgctga gctgaacata tcaacatcag gcaacggcac aatctgtgta caaactccgc
     3181 cagccctcag ataacacatc actgagtaaa tggtcaggta ttgcttatat tatgcccctc
     3241 tgcatctatt attgctgcca tccactgagc ttctgaggcc gacaatttta gccgtggagc
     3301 tcgctccccc gcaccaggtg gcttcatatc aaaaccataa gagcttctgg atataaccgt
     3361 tataccatcc acattacatg ttgttgatat cagttcacaa tttttagttg atggcttatc
     3421 aattattgga tcagatattt ctggtcgacg tcctgtgtga catgaaaaac aacttccgat
     3481 tgacaatggc ataattttat ctcctgccga tattacttta ctgatatgca ctg
//
LOCUS       DAZFYC010000252         3529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFYC010000252 DAZFYC010000000
VERSION     DAZFYC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.258"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            96..746
                     /gene="zinT"
                     /locus_tag="ACHKW5_005222"
     CDS             96..746
                     /gene="zinT"
                     /locus_tag="ACHKW5_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO7514386.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1089..1619
                     /gene="yodB"
                     /locus_tag="ACHKW5_005223"
     CDS             1089..1619
                     /gene="yodB"
                     /locus_tag="ACHKW5_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7514387.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            2460..2548
                     /locus_tag="ACHKW5_005224"
                     /pseudo
     CDS             2460..2548
                     /locus_tag="ACHKW5_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(2964..>3529)
                     /gene="nleF"
                     /locus_tag="ACHKW5_005225"
     CDS             complement(2964..>3529)
                     /gene="nleF"
                     /locus_tag="ACHKW5_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO7514388.1"
                     /translation="PTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLTPELQDKL
                     DAMISIYSNARNSNELEEIYQELSAFVSGLMDKRNSVFEVRNENTDEVVGALREGMTI
                     DDRDGYIRELFFLYSLKVKIEESRQGKEDSKCKVYGLLCPHHSSELYGDLRAMKCLVE
                     GCSDDFDPFDIIRVPDLTYNKGALQCG"
BASE COUNT         1097 a          760 c          640 g         1032 t
ORIGIN      
        1 aaactatgct ctcatctcct aagagttgtt gcattttgcc atatgttaca atataacatt
       61 acacatcata tacaatgact ctggaggaaa ctgttttggc gattcgtctt cacaaactgg
      121 ctgttgcttt aggtgtcttt attgttagcg ctcctgcctt ttcgcatggt catcactcac
      181 acggcaaacc cttaacagag gtcgaacaaa aagctgctaa tggtgttttt gatgatgcca
      241 atgtacaaaa ccgaactctc agtgactggg atggagtctg gcaatccgtt tatcctttac
      301 tgcaaagtgg caaacttgac cccgtctttc agaagaaagc ggatgcagat aaaactaaaa
      361 catttgctga aattaaagat tattatcaca aaggttatgc aacagatatc gagatgattg
      421 gcattgagga cggcattgtt gaattccata gaaataatga aacaacatcc tgtaaatatg
      481 attacgatgg atacaaaata ctcacctata aatcaggcaa gaaaggcgtt cgttacttat
      541 tcgaatgtaa agatcctgaa agcaaagccc ctaaatatat acaatttagc gatcatataa
      601 ttgcaccacg aaaatcatct cattttcaca tttttatggg taatgattca cagcaatctt
      661 tattaaatga aatggaaaac tggccaacgt attatccata tcagttgagt agcgaagaag
      721 tggtcgagga aatgatgtct cattgagtat tctcatgata acgcctcgat gccgctttag
      781 taagtcatca taactgccac tggtccatcc acaaacgcca ctgaacgcaa gctagctaca
      841 gacacgctca tcactatgac gtgtctgtat attaataagc taacccgcat tgagttaacc
      901 aataacggat tccatacaca atacggccaa cagagaaaga tacctgtgct cacgccattg
      961 cttatattgg ctggttacaa tgtgcactat caatttttta aataacaaat tcttaaaacc
     1021 acatctgtat aaggattttt taaggttcgt gggatagctt gactgtaaaa attacaggag
     1081 ctacaaaaat gaaccgattc tcaaaaactc aaatttattt acattggata acgctgcttt
     1141 tcgttgcaat aacctatgcc gcgatggaac tccgtggctg gtttcctaaa ggtagtagta
     1201 cttatctgct gatgcgagaa acacattaca atgcgggtat attcgtttgg gtgttaatgt
     1261 tttcacgcct gattataaaa caccgttata gtgatccttc tattgtgcca ccgccacctg
     1321 cctggcaaat gaaagcggct tcgctaatgc acatcatgct ttatataacc ttccttgcac
     1381 ttcctctgct ggggattgct ttgatggctt acagtggaaa atcgtggagt ttccttggtt
     1441 tcaatgtgtc tccctttgtt accccaaaca gcgaaattaa agcactgata aaaaatattc
     1501 acgaaacctg ggcaaatata ggttactttt taatcgcagc tcacgctggc gcagcactct
     1561 ttcatcacta cattcagaaa gataatactc tgttacgaat gatgcctcgc cgcaaataag
     1621 tcgatcccct gacaaggaaa tgtgaaccat ggacatcaac gcgtcataaa tgtttacgca
     1681 agtgcacaat gcaccctttc cactatcaac aaggaggagc agaaactgtg taaatcgtac
     1741 aatggtaaga cttatcaaaa atacaggtta attttggtat ttaaaccttt tttacttgtt
     1801 agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg aaattatgga
     1861 ctttgtcccg cggctttaat ctgacggccg cgttactttt ttgactcaat tattgacccc
     1921 ttctctacgc aacttcagtt cccaccacca actttgcggc agtttcatag gatgaatgtc
     1981 taaaagaata atggtgagcg ataagaaaac gactgaataa ctgcacattt tcgctcgaaa
     2041 gcttcctgtc agatccatag cgaatcaagt gctgaatgtc acagtatcga acagaaaaca
     2101 gtgacgatct aacccttcaa gaatattcta cgattgttct gtttaggaaa agcaaggcgg
     2161 gaagtcggga gataagtcat tgataaagtg gcggagagag ggggatttga acccccggta
     2221 gagttgcccc tactccggtt ttcgagaccg atgataccga atcgcaaaat caaaaggtta
     2281 taaatttttc ttggaataaa tgtcttaaaa acatacacga aaaatcaata aactacgttg
     2341 aattcaataa gaatattcca agccttcttt gagttatttt tgaccagttt gctgtttttc
     2401 tttctcataa aatatttcct gatatccctt aacattactg atactcaagt agtgaattta
     2461 tgagcaatcc taatccatgc atgacgtgtg gtgcctgttg tgcatttttc gcgtctcttt
     2521 ttactgggct gaagcagatg atgctggcga aaggtttatc agaaaaccgt agcgccttct
     2581 tgcatacgac gtgtccgcag ttttatataa tcccgaccag aaacctctta taaaaagtcg
     2641 atacacaaat atcatggata atcaccacct tctgacgaag aatatctgat gaaattaatc
     2701 gcccctctcc tgagaccatt gcgctggggt aaatttgtaa ctacgtgtgt acagtgaggt
     2761 gtcgtatagc acgccgtcag gaaatgcaag gtgcccccga tatccccgac tacaagtcag
     2821 caaacaaagt gattggctgg caatccacat cacagaaatg gacaatgcta ctttataatc
     2881 gagcttggta gccaccttga tggtcacttt taaaaaaagc gtttacacaa aatgataagc
     2941 aggctcacag gacctccctc taatcatcca cattgtaaag ctcctttgtt gtaagtaaga
     3001 tccggcaccc taataatatc aaaaggatca aaatcatcac tgcatccttc cacaagacat
     3061 ttcattgctc gtagatcacc atatagctct gaagagtgat gcggacataa cagaccataa
     3121 actttacatt tagaatcttc tttaccttgt ctactttcct caatttttac tttcaatgaa
     3181 tacagaaaaa aaagctccct gatataacca tccctgtcat ctatcgtcat cccctccctc
     3241 agtgctccga caacctcatc agtattttca tttctcacct caaatacact atttctctta
     3301 tccatcagcc cacttacaaa agcacttagc tcttgataaa tttcctcaag ctcattacta
     3361 tttctggcat ttgagtatat agagatcata gcatcaagtt tatcttgtaa ttcaggagta
     3421 agaaaatgat tatgattaag ttcacttact gattccggag tcgaagggtt acctcttcct
     3481 gatacataca tccatgaata aagatttgct gaagagccac ttgttggta
//
LOCUS       DAZFYC010000253         3414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFYC010000253 DAZFYC010000000
VERSION     DAZFYC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.259"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..342
                     /locus_tag="ACHKW5_005226"
     CDS             <1..342
                     /locus_tag="ACHKW5_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO7514389.1"
                     /translation="KKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPNVAISLDRT
                     EGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPGI
                     ITGSLKTPPLA"
     gene            393..926
                     /locus_tag="ACHKW5_005227"
     CDS             393..926
                     /locus_tag="ACHKW5_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7514390.1"
                     /translation="MNTKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GTGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1197..1751
                     /locus_tag="ACHKW5_005228"
     CDS             1197..1751
                     /locus_tag="ACHKW5_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO7514391.1"
                     /translation="MNMMAVPFYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKLRQRFA
                     STITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEECDDVLY
                     EYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVSKIRTL
                     VNEANMLIDFVLADTGKGKITKAD"
     gene            1751..1897
                     /locus_tag="ACHKW5_005229"
     CDS             1751..1897
                     /locus_tag="ACHKW5_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514392.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            2125..2310
                     /locus_tag="ACHKW5_005230"
     CDS             2125..2310
                     /locus_tag="ACHKW5_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7514393.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(2735..2962)
                     /locus_tag="ACHKW5_005231"
     CDS             complement(2735..2962)
                     /locus_tag="ACHKW5_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7514394.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            3004..3369
                     /locus_tag="ACHKW5_005232"
     CDS             3004..3369
                     /locus_tag="ACHKW5_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7514395.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT          879 a          745 c          951 g          839 t
ORIGIN      
        1 aagaaaaaat acgaactggt tgttaaaggg ataaataatt acccggataa gattactgtt
       61 actgtggcgc tggaaattgg tgggtatccg tcactgttgt tgccaaatgt ggcgattagt
      121 cttgaccgta ctgaaggtgc cacgctggag ttttacgaag cggaggcgaa aaagcaggcg
      181 aagcagtttt tcatggatgt tgctgccggg ttatgtgaag gggatggtcc gttgccggaa
      241 aagcggccca tcattttaga ggcgcaggat gtgttgataa cctacagagg aaaactaccg
      301 ggaataatta ctggttctct gaagactcca ccgctggcct gaagacttaa catatccagg
      361 gatttgaaat cgataaaccc tgataaatat ccatgaacac caaaatcaaa tacggcctgt
      421 cggctgccgt tctggcgctg attggtgcag gggcgtctgc gcctgaaatc ctcgaccagt
      481 ttctggatga aaaggaaggt aaccacacca cagcataccg tgatggtacg ggtatctgga
      541 ccatctgccg cggtgccatc ctggtggatg gtaaacctgt cgtcccgggc atgaagttgt
      601 cgaaggaaaa atgcgaccag gttaacgcca ttgagcgtga taaggcgctg gcatgggtgg
      661 agaaaaacat caaagtgccg ctgaccgaac cccagaaagc ggggatcgcg tcattctgtc
      721 cgtacaacat tggtcccggt aagtgtttcc cgtcgacgtt ttacagacga attaatgcag
      781 gtgatcgcag gggagcatgt gaggcgattc gctggtggat taaggacggt ggcagagact
      841 gccgtattcg ctcaaacaac tgctacggtc aggtctcacg gcgtgaccag gagagcgcgc
      901 tggcgtgctg gggaattgac agataagaag aatattttgc tgaaaaatga ggtttgctta
      961 catggacgga taacacgaaa tcctgcaaat tggcaaaatg taagtgaata aagtcaaaac
     1021 agttgtttaa cactcaggca ccgtaatgat gcctttgtca tttctgcgca tctcacgcgc
     1081 atctcacaac acagaacctt tcaggatgac ccttgaggat accggtttgg ctgtcggtgc
     1141 ctttctgtgg gctggattcc tgtgagacaa ggttcatcac taaaaggaaa taaccgatga
     1201 atatgatggc cgtgccgttt tatgtagtta accataatgg cgaaccatac gttcccatga
     1261 aacctgtcgt tgcggggatg gggctggcct ggcaatcaca gttggctaag ttaagacagc
     1321 gttttgcgtc aactataacg gaaatcgtta tggttgctga ggatgggaaa caacgcaata
     1381 tggtgtccat gccacttcga aaacttgccg gctggctaca aaccattaat cccaacaaag
     1441 taaaacccga aatccgcgat aaggtcatcc ggtatcagga agagtgcgac gatgttcttt
     1501 acgagtactg gacgaagggt tttgtcgtta atccccgtaa aatgagcgtg atggaagaac
     1561 tcaaccaggc ttgtgctgac atgaaacggg ataaaaacat tgccagtgtg tttgctaccg
     1621 ggctgaatga gtggaaacag gttaaagccg cgcatgtatc aaaaatccgt acgctggtaa
     1681 atgaagcgaa tatgctgatt gattttgtcc tggctgatac aggcaaaggg aaaataacaa
     1741 aggcggattg atggggtggc taatgatatc agataaactc ataacgctgg tgaagagcct
     1801 ctgtgtactt gtcggcattt catttttagt catgctggtt gccattttct tttccaccgc
     1861 ctggcgagtc ctgacgttat cgggactggt ggggtgaaag agagatgaac cgtgttctgt
     1921 gtgtggtgat tattgtcctg gcggttggct gtggtgcgct gtggctggca acaaaccatt
     1981 accgtgacaa cgcgctcacc tacaaagcgc agcgcgataa aaaagccaga gagctggaac
     2041 aggcgaatgc caccattact gacatgcagg tgcgccagcg tgatgttgct gcgctcgatg
     2101 caaaatactc gagggagtta gccgatgcga gagctgaaaa tgaaactctg cgtgctgatg
     2161 ttgccgctgg tcgtaagcgc ctgcggatca acgccacctg ctccggtacc gtgcgtgaag
     2221 ccaccggcac ctccggcgtg gataatgcaa ccggcccccg actggcagac accgctgaac
     2281 gggattattt catcctcaga gaacggttga tgacaatgca gaagcagctg gaaggggcac
     2341 aggactatat ccgcactcag tgcctgaact aagttttgct gatgcgccgt atcgtcgccg
     2401 tattcctgca ttaacagaga ccgcagcccg acagggagac tcctctgcga gagtgtgcgg
     2461 ggataatcaa aaacgataca caccggggtt taccgcgtaa acggagcgcg gcgttctccc
     2521 ctcatggtcg cccgtccggt gcgatggtgg aagaaactgg aatctgttca ataaaaaaac
     2581 tgccgtgttg gagtcacagc agtaatgtac tgattgggta gaagattatt attgttatgc
     2641 tttattttta ttctatatgg ctgattattt caattcggaa ttaatacagc taatgtctgt
     2701 gagtttttat aaattcagca atataaagaa atagttatat gaacagccat cgcagagcat
     2761 actgtgtatc attctttttt atagtcaact gacgggcata ttttatgtct gctgccagct
     2821 cccggcggca agattcaatg acccacgcag aaaaattttc tgaacctttc tggtcaagag
     2881 cgatgttaat ttgttcaatc atctggtttg gaaatcggat gttgcgggtt gttgttctgc
     2941 ggggccggtt tttcgatgac attttctttc ctctggtgac aagctatatg gcgaggattt
     3001 tacatggctg tgcttcgtac gttaccgggc agaatcaaaa ctctgaacac ccggcgggtg
     3061 aatattctga agggtgaaca gcgtcgtgtc agtggcagtg cacgtgtttc cctcaagcgt
     3121 catatctggc tcagggatgc cgggcagtgc tgtctctgtg gtcgtgtggt tgacctctgt
     3181 gacagtgaac tcgatcaccg aattgcactt cagttcggtg gtggtaatga ggagacgaat
     3241 ctctggacgc tctgtaccga atgccatcga caaaagtctg ctcgtgaagc ggcgggtggt
     3301 atgccggacc cgacgctgcc ggaggtgtcc ggaggtcatg gcagggcaga cgatatcatc
     3361 ggactgtgac ccgccccggg gggggggatc atccggcgaa aaaaacgatc gctc
//
LOCUS       DAZFYC010000254         3414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFYC010000254 DAZFYC010000000
VERSION     DAZFYC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.260"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..835)
                     /locus_tag="ACHKW5_005233"
     CDS             complement(<1..835)
                     /locus_tag="ACHKW5_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7514396.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSVSHILAVRKSTLLQIDTLIRQ
                     LAEISVMTESIGGKTALDWAMKQDFRCGCWLMEKTETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VL"
     gene            complement(920..1117)
                     /locus_tag="ACHKW5_005234"
     CDS             complement(920..1117)
                     /locus_tag="ACHKW5_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7514397.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLPDQNR"
     gene            complement(1129..1617)
                     /locus_tag="ACHKW5_005235"
     CDS             complement(1129..1617)
                     /locus_tag="ACHKW5_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7514398.1"
                     /translation="MNLSLTLEADSLNVQALNMGRIVVDVDGIELAELINNVTENGYS
                     VRVVEDSGQQSTCTPPPLTILAGIRCSTAHITETDNAWLYSLSHQSSNFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(1614..1991)
                     /locus_tag="ACHKW5_005236"
     CDS             complement(1614..1991)
                     /locus_tag="ACHKW5_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7514399.1"
                     /translation="MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRSHYRTVNDITLGKRTEAKR"
     gene            complement(2038..2412)
                     /locus_tag="ACHKW5_005237"
     CDS             complement(2038..2412)
                     /locus_tag="ACHKW5_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7514400.1"
                     /translation="MSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            complement(2487..2708)
                     /locus_tag="ACHKW5_005238"
     CDS             complement(2487..2708)
                     /locus_tag="ACHKW5_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7514401.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            complement(2777..3253)
                     /gene="radC"
                     /locus_tag="ACHKW5_005239"
     CDS             complement(2777..3253)
                     /gene="radC"
                     /locus_tag="ACHKW5_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7514402.1"
                     /translation="MQQLSFLPGEMTPGERSLIHRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPGKTDRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(3269..>3414)
                     /locus_tag="ACHKW5_005240"
     CDS             complement(3269..>3414)
                     /locus_tag="ACHKW5_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7514403.1"
                     /translation="AGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMR
                     IID"
BASE COUNT          773 a          855 c          939 g          847 t
ORIGIN      
        1 agagcacaga aggaaagtac ctggctatta tatcgttaat tctgtcaatc agttccagcc
       61 ttttgaaagt gatgtgtgca gatcccttct taaagtattt aatgatgaac atctcgtctt
      121 catagcgctc tttaccctga acggactgga tgtgaccatc cagacgaata gtgatgtttt
      181 cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc aggtcgacga
      241 gtcggtcagt ctgttgcccg gtgataagat gaaaccccca ccggtcccac ctcactaggt
      301 tgttgacgat aattttactg ccaaatttac aggggcaatt ggttttgtaa ttccagctca
      361 gccctctgaa gaggttgatc acacctcgct caaatacttc gtccttattc tggtgcagtt
      421 gttcaaacgt actcaggata ttcgcctcgc tgatttccgg aaaattatcg tactccagtg
      481 atcggtacca tgtatcacgt gcctgtgcat ccattaatga cagcatccct gaacgttgca
      541 tcaggtcacg ccagatttcg cggtcaaggt tgcgggtgat agctttcatt gcggtttccg
      601 ttttctccat cagccagcag ccgcagcgaa aatcctgttt catggcccag tccagtgcag
      661 ttttaccgcc aatgctttcc gtcataactg agatttcagc cagttgccgg ataagtgtgt
      721 cgatttgcag cagtgtactc tttcgtacag ccagaatgtg gctgactgag gttgagcaaa
      781 taacgtccgt atgcccgttg accacttccg gacgggaggc tgtgatatca gacataatcg
      841 atattccttt ttacagataa acgaaaacgc cagccgtatt caggctggcg tcagtggaat
      901 gaagagtggt gaatgagact caccggttct ggtctggaag ctgcagctgg cgcagggtac
      961 ggatatcctc cctggcctgt tcaatacagg gcaacagtgc tgccgaatcc gttttatctt
     1021 catcgttgtc aaagataatt cccgattcgc agtcgatatt gtcctgcagc catgcaatca
     1081 gaatatccag cgctgtttcc gtggttaatg atttcatgtt gtttctggtt accagtcgaa
     1141 agtgggtaaa tccggcagac attcggcgct ggcatccaga tgaatgagac tgacgccata
     1201 acgtcgggta agtgtgacaa ccagacggcg gaacgttctg gacaggccca ggcgtttaag
     1261 ccgcagaacc gggtatgacc acgcatcagt acgtaacaga tatccgctac ctgtaaaatg
     1321 aatccattct gattcgccga agttactgct ctgatgcgac agcgagtaca gccaggcgtt
     1381 gtctgtttcc gtgatatgtg cggtgctgca gcgtatgccg gcaaggattg ttaacggtgg
     1441 tggtgtgcag gttgactgtt gcccggagtc ctccaccacg cggactgaat aaccgttttc
     1501 agtgacattg ttaatcagtt cagcgagctc aataccatcg acgtcaacga caatgcgtcc
     1561 catgttcagt gcctgtacgt taaggctgtc ggcttcgagc gtcagggaaa ggttcatcgt
     1621 tttgcctccg tgcgtttacc cagggtaatg tcatttaccg ttctgtagtg gctgcgggtc
     1681 atcaggccgg ttgcccggcg ggcccggaga atatcaatgc tgttgattaa ctgcgacggg
     1741 gctcctgcgc tgaatcccgg ctggtcagtt cgtaccagcg cgtatttttc aacgagaaag
     1801 ttcaccgcat cacacagtga aatccctgcc tcaatatgct gctcaatcac acgttcatca
     1861 gcgaacggtg tgtcattcag cgtgaggcca tagtgctggt ccagcagtcg ggtgagcagt
     1921 gtctgccaga tggtgacggg agacgggcag cccgatgcct cccgtacgtg tgtgtcaggt
     1981 aatgttttca tcttaaattc tctgtaactg gtggtgctaa aggtgagata ttcggggtta
     2041 tgaggtggtt gcgggtgcca gtgtcggata aacagccata taaacgtaac cacagctgcc
     2101 gagggtgtcg gcttcgcagg tcagccctct ggcatacagc gtgacggtat gctgatggcg
     2161 gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca gcggaaaggc
     2221 ctggtccaga tgatacgcat ccgcgtcgct aaactgcccc ctgataccgg cgcggtcggc
     2281 aaggtaatgt aattgattgc cctcctgcac cagacgtgcc ccgaaacagg gcgtcacggt
     2341 gcaggggagc ccccaccagg ggcggtcgtt gttgtcgttg ggataatttg tctcatggag
     2401 tgtgcctgac acgataaaat cctcacaaaa aatcagttaa gaatggtccg gtggcagcga
     2461 taattctgct catcatttct cctgactcag ttcagtgtga cgctcatcag ccggacatac
     2521 gagccaaaac tgtccttacg gcgttctgca aacacggcaa gaacgccggg aatatcctgt
     2581 acttcgcgac cggtatacgc ctcagcgcta ccgtgccagc gatacttacc ggtacagaac
     2641 ggaaaaagac gggatgcagg atgctgttgg tgaatacgca tggcttcacc acgggtgatg
     2701 attctcatga tgtgatacct ctgaagacag aagataaaag cgaaaacagg tatgatgtgg
     2761 tggtgacggt gacgggttaa agcagaccgt gttccgcaaa ggagaaaacc tggctgccac
     2821 cgactatcag atggtccggc acccggatgt caaccagggc cagcgcctgt accagacgtt
     2881 cagtgataag ccggtcagtc ttaccaggtg tgacttcacc ggacgggtga ttgtgtgcca
     2941 gcaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg ggatggactt
     3001 ccgtgcggtt gatggtgccg gtgaagaggg tttcaccggc aatcagctga ttctggttgt
     3061 tcagatacag cacccggaat tcttcacgct ccagtcctgc catgttcaga atcagccatt
     3121 cccgtgccgc acgggtggag gtgaaggcca cgccgggttc atgaagatgg cggtccaggg
     3181 ttttcagggc ccgatgaatg aggctgcgct cgccgggcgt catctctccg ggcagaaagg
     3241 aaagctgctg cattgtccct gcctccattc agtcgatgat gcgcataatg gcgctgcatt
     3301 ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg gaccgtcatg gcataattct
     3361 ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc ggcaataccg gcgg
//
LOCUS       DAZFYC010000255         3406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFYC010000255 DAZFYC010000000
VERSION     DAZFYC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.261"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2805
                     /locus_tag="ACHKW5_005241"
     CDS             <1..2805
                     /locus_tag="ACHKW5_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7514404.1"
                     /translation="LASENPDEAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDS
                     QPGTLNDFLGAMTEDDARPEALRRFEQMVEEVARNASVVAQNTAAAKKSASDAGTSAR
                     EAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEAEKSAAAAESSKSAA
                     ATSAAAAKTSETNAAASQQSAATSASTATTKASEAATSARDASASKEAAKSSETNASS
                     SASSAASSATAAANSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAASTSA
                     GQASASATAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETS
                     AESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAA
                     QSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESLAATPK
                     AVKAAYELANGKYTAQDATTAQKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYT
                     AQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSARKVNGKALSADI
                     TLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGI
                     SELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWD
                     YTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYAL
                     MQGQTFDKSAYPKLATAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASA
                     SSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGS
                     VHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNA
                     AGNAENTVKNIAFNYIVRLA"
     gene            2805..3380
                     /locus_tag="ACHKW5_005242"
     CDS             2805..3380
                     /locus_tag="ACHKW5_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7514405.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSID
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
BASE COUNT          912 a          884 c          955 g          655 t
ORIGIN      
        1 ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga gtacgggcag
       61 tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac catcaccgtg
      121 tatgaagatt ctcaacccgg tacgctgaat gattttctcg gtgccatgac ggaggatgat
      181 gcccgtccgg aggcactgcg ccgttttgaa cagatggtgg aagaggtggc gcgtaacgcg
      241 tccgtggtgg cacagaacac ggcagccgcg aagaagtcag ccagtgatgc cggcacatct
      301 gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc agccagcacg
      361 tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg aacggcatca
      421 acaaaggcca ctgaagcgga aaaaagtgct gccgctgcag agtcctcaaa aagcgcggca
      481 gctaccagtg ccgctgccgc gaaaacgtca gaaacgaatg ctgcagcgtc acaacaatca
      541 gccgccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac ctcagcccgg
      601 gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc ctcgagcgcc
      661 agtagcgcag cttcctcggc aacggcggca gcaaattccg cgaaggcggc aaaaacgtcc
      721 gagacgaacg ccaggtcttc tgagacggca gcgggacaga gcgcctcagc tgcggcaggc
      781 tcaaaaacag cggctgcgtc gtctgccagt gccgcgtcaa caagtgccgg gcaggcctca
      841 gccagtgcca ccgctgccgg aaaatcggca gaaagcgccg catcgtccgc ttcaacagcc
      901 acaacgaagg ctggcgaagc cactgaacag gccagcgcag cagcgaggtc tgcttccgca
      961 gcgaagacat ccgagacgaa cgcgaaagcg tcggaaacca gcgcagaatc ctcaaaaacg
     1021 gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catctgcgtc tgcttcaaaa
     1081 gatgaggcga ccagacaggc gtcagcagca aagggcagcg ccacgacggc atccacgaag
     1141 gcgacagagg cagctggcag tgcgacggcg gcagctcaga gcaaaagtac ggcggaatcc
     1201 gcggcaacgc gcgccgagac agcggcaaaa cgggcagagg atattgcatc cgccgtggcg
     1261 cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagcagtgc gactaacagc
     1321 acttccgagt cactggcggc aacgccaaaa gccgttaagg ccgcgtatga gctggctaac
     1381 gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggatagttca gctcagcagc
     1441 gcgaccaaca gcacatctga aatgctggcg gcaacgccaa agtcggtaaa ggcagcctat
     1501 gaccttgcta acgggaaata tactgctcag gacgctacga cagcacaaaa aggaattgtc
     1561 cagctcagta gtgcaaccaa cagcgcatct gaaacgcttg ccgcgacacc gaaagcagtg
     1621 aaagcagcta atgataatgc gaatggtcgg gtaccttctg cccgtaaggt gaatggtaag
     1681 gcgctttcag cggatataac actgacgccg aaagatattg gtacgcttaa ctcaacaaca
     1741 atgtcattca gcggtggtgc tggttggttc aaattagcaa cggtaaccat gccacaggcg
     1801 agttctgttg tttcaattac gttgattggt ggcgcgggat ttaacgtggg gtcacctcaa
     1861 caggcaggta tatctgaact tgttttgcgt gcaggtaatg gtaatccgaa ggggattact
     1921 ggtgctttat ggcagcgcac atcgacaggg tttacaaatt ttgcctgggt caatacatct
     1981 ggtgatactt acgatattta cgttgcaatc ggaaattatg cgactggtgt aaatattcaa
     2041 tgggattata ccagtaatgc cagcgtgact attcatacgt caccagcata ttctgctaat
     2101 aagccggaag ggttaacgga cggtacagtt tattcactct atacaccatc agagcagttt
     2161 tatccgcctg gcgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg
     2221 atgcaggggc agacttttga caaatccgct tacccgaaac ttgcaaccgc ttatccgtca
     2281 ggcgtgatcc ctgatatgcg tggctggacg attaagggca aacctgccag tggtcgggcc
     2341 gtattgtctc aggaacagga cggcattaaa tcgcacaccc acagcgccag tgcatccagt
     2401 acggatttgg ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac
     2461 accggggcgc atacgcacag tgtgagcggt acagccgcaa gtgccggaaa ccatactcat
     2521 agtgtcacag gcgcatcagc agtcagccag tggtcacaaa atgggtcagt acataaggta
     2581 gtgtctgcgg ccagtgtgaa tacaagtgct gcaggagcgc acactcatag tgtcagcggc
     2641 acagctgcat ctgcaggtgc tcacgcacat actgtcggta ttggtgctca tacgcactct
     2701 gttgcgattg gctcacatgg acacaccatc accgttaacg ctgctggtaa cgcggaaaac
     2761 actgtcaaaa acatcgcatt taactacatt gtgaggcttg cataatggca ttcagaatga
     2821 gtgaacaacc acggaccata aaaatttata atctgctggc aggaactaac gaatttattg
     2881 gtgaaggtga cgcatatatt ccgcctcata caggtctgcc agcaaacagt atcgatattg
     2941 caccgccaga tattccggca ggcttcgtgg ctgttttcaa cagtgatgag gcatcgtggc
     3001 atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat
     3061 ttatttctga actcggtccg ttaccggaaa atgttacctg gttatcgccg ggaggggaat
     3121 atcagaagtg gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgga
     3181 tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catattgcgc
     3241 cgcttcagga tgctgtggat ctggagatcg caacggagga agaaaccttg ttgctggaag
     3301 cctggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca ccggatattg
     3361 aatggccagc cctaccgtag ggtaagatat ataaattcta taatta
//
LOCUS       DAZFYC010000256         3350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFYC010000256 DAZFYC010000000
VERSION     DAZFYC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.262"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2051
                     /locus_tag="ACHKW5_005243"
     CDS             <1..2051
                     /locus_tag="ACHKW5_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO7514406.1"
                     /translation="KSRKSVKSVEPEITELANSWLKSYRLDYKLQNASLNGEIDNALD
                     AYFSKNGGAGGNRPDVKLLVKDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDGTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDARPTLEDFKKTVGDYLAWEVSQLLKKQGENNFAGK
                     "
     gene            2093..2554
                     /locus_tag="ACHKW5_005244"
                     /pseudo
     CDS             2093..2554
                     /locus_tag="ACHKW5_005244"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            2645..3205
                     /locus_tag="ACHKW5_005245"
     CDS             2645..3205
                     /locus_tag="ACHKW5_005245"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7514407.1"
                     /translation="MEFSGFPVTELFSVCNTGNILSRDIIENSGDVPYLCASRDNNSV
                     SSYIAYDDSLLEKGNCIFIGGKTFVVTYQERDFFSNDSHNLRLYVNNEDGRTKFAYLG
                     IISCIYRSISHKYSWGDSVSNRKIKNDVIWLPVKNKSPDFCYINDLISVVHKLVIKDV
                     VQYAERKMNAYRQAITSRGYDDSGVS"
BASE COUNT         1033 a          594 c          785 g          938 t
ORIGIN      
        1 gtaaatcaag aaagtctgta aaatctgttg agccggaaat taccgaactg gcgaactcat
       61 ggcttaaatc ttacaggctt gattacaagt tgcagaatgc atcacttaac ggagagatcg
      121 ataacgcact ggacgcttat ttttcgaaaa atggcggcgc aggcggtaat cgtcccgacg
      181 taaaactcct tgttaaggac aaatacggca agcagtatcc cgttctcatc gagtataaag
      241 gctacaagga caggcttatc agacttggca gcaacggtat tattgagaac aaagatgcca
      301 gaaaagaatg gaattttaaa aatattaatg gatacgcggt gaatggcgca gtccattatg
      361 ccaacgcact tcttcagttc accaattacc ctgatattat tgctattggt atgaccggct
      421 ggcgcgatga tggtacaggc gaactgcatc atgaaatcgg cgtgtggtac gtatcaaaga
      481 ataatttagg agccggacag aaggttggtg aattcaccga cttatcgttc cttgctgata
      541 aaaatgttga tggatttctc aacaagataa agctgcttaa tcttcctccc gaagaactgg
      601 aaaaaatcaa ggccagcaaa gaggaagaga tagacacgcg actttcccgt ctgaataacg
      661 atatttacca gaatgaaaag gggcttggtg aaagtgatcg tgtttacctt gttgtggcta
      721 ccgttattgc aacgttgggg attccgggaa agttagcacc gctggataaa aaagaactga
      781 cctcttcaac ggaggaggac ttgcgtgatg gtgatattat tttcaggaaa ataaggaatt
      841 ttttaaggct gaaagctgtt cctgaaacaa aaagagaaat gattttgcgc tcgttgcaaa
      901 atacgctgtg gactgaaaac attaacaaac cagtcaacgg tgaaagccag ttaaagcgcg
      961 tgtttgttaa agttgttgat gatcttgggg agtattacaa aattggactg accaccgatt
     1021 ttacgggcaa actgtttaat gagatgtatc gctggcttgg atttacgcag gacaagctca
     1081 atgacgtcgt tctgacacca ccttatgttg ccacattgct ggcacgactc gccagagtaa
     1141 acaaagattc ctatgtgtgg gattttgcta caggttctgc gggattgctt gtggctgcaa
     1201 tgaatgaaat gctcattgac gccagagaaa atattcactc accgaacgaa ctacaactta
     1261 aggaagcgca aatcaaggct gaacagcttc ttggccttga ggtgttatcc agtatttata
     1321 tgctggccat cctgaatatg attttgatgg gggatggtag ctcgaatatc ctcaataaag
     1381 actcgctggc cgattttgac ggcaagtatg gatttgggaa aacaggagaa aaattccctg
     1441 cggacgcatt tattctcaat cctccgtatt cagctaaagg taacggcatg attttcgtgc
     1501 agaaggcgct gtcgatgatg gataaaggct atgctgcagt gattatccag agttcagctg
     1561 gcacaggaaa ggctacggag tacaacaaaa aaatactcaa ggaaaatacc ttgctggcaa
     1621 gcatcaaaat gcctgcggat ctgtttattg gtaaatcaag cgttcagacg tacatttacg
     1681 tttttcaggt aaaaattccg cataacgcga aacaggctgt taagtttatc gacttttcca
     1741 atgacggcta cgctcgctct aatcgcaaaa aggcaagaaa caatcttgtt gatgccgatc
     1801 gtgcaaaaga gcgttatcag gaggttgtgg atctggttca cttcgggaaa ggttgcctta
     1861 atatctttac ggaggatgaa tattttgagg gaacaatcga tcctgatagt ggtgaggact
     1921 ggaatcagac caggccagta gatgctaggc caacgctgga ggatttcaaa aagacggtag
     1981 gtgattacct tgcatgggag gtctcgcaat tactgaaaaa acaaggtgaa aacaattttg
     2041 cgggaaagta aattccccgc tcagcgataa attaagaagc gttgagtggg gagagttcag
     2101 gattggcgat ttgttctatg tgagcacagg ctcgcttatc tcctcaggaa gattaaaacc
     2161 aggcaaaatc aaacgtgttt cagcaaagag cgatcaaaac ggtatcatcg gagagtttga
     2221 taccgagttt atggatgatg caaggcatta tgaaaatttt ataagcgtta atttttttgg
     2281 agatgctttt tatcatcctt attgtgccag cgtagaaatg aaggtgcatg ttcttacatt
     2341 aaaatcaagt aagtccgact ttaatgcaaa gcgcggactg tatgttgcca gtatgattaa
     2401 taaagcttta aaggggcgtt ttaattatgg tcaacagtta tcgagttcca aactcagaga
     2461 tggagaatat ttcatctcat taccagtgaa aaataatgaa attgactggt tttttatgga
     2521 taattatata gaagagctgg aagcggccca tatagaagag ctggaagcat acctgatagc
     2581 tgctgggctg agtgattacc aactaacgga aaaagattat cttatcctgg agcattttaa
     2641 aaatattgaa ttttcaggct ttcctgttac tgagttattc tcagtgtgca atacaggaaa
     2701 catattgtct agagacatca tagaaaatag tggggatgtt ccttatcttt gtgctagccg
     2761 tgataataac tctgttagtt cttatatcgc ctatgatgac tcactcttgg aaaaagggaa
     2821 ctgtattttt attggtggta agacattcgt tgtaacttat caggagcgag actttttttc
     2881 aaacgacagt cataatttgc gactctatgt taacaatgag gatggaagga caaagtttgc
     2941 ttatttgggc attatttcat gtatttatag gtctatttca cataaatatt catgggggga
     3001 tagtgttagt aatagaaaaa ttaagaacga tgttatttgg ttgccagtga aaaataagtc
     3061 acctgacttt tgttatataa atgaccttat ttcagttgtt cataagcttg tcattaaaga
     3121 tgttgtccag tacgcagaac gcaaaatgaa cgcatacagg caagctataa caagtcgcgg
     3181 ttatgatgat tctggtgtca gttaaaaaac attttacaat cgtaaaaatc ctcatatcat
     3241 gataagaatg gttacattgc cacgctgctt aacccgccga tgcgcgggtt ttttgtatcc
     3301 ggaattctgt gggttaccct gcccccctta gaaaggtgcg acagcaatta
//
LOCUS       DAZFYC010000257         3332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFYC010000257 DAZFYC010000000
VERSION     DAZFYC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.263"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..528
                     /locus_tag="ACHKW5_005246"
     CDS             <1..528
                     /locus_tag="ACHKW5_005246"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO7514408.1"
                     /translation="AVKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQAALNEF
                     ATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEV
                     APSIIKENLSLHLTHTVKWTESFDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVVQQR
                     VMHCRKAFSDAKVFK"
     gene            525..1511
                     /gene="ycdU"
                     /locus_tag="ACHKW5_005247"
     CDS             525..1511
                     /gene="ycdU"
                     /locus_tag="ACHKW5_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO7514409.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            1878..3080
                     /locus_tag="ACHKW5_005248"
     CDS             1878..3080
                     /locus_tag="ACHKW5_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514410.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          860 a          651 c          829 g          992 t
ORIGIN      
        1 gcggttaaag gcatcaaccg cgaacaactg cagacgatga ttacggaatc aggcattgcg
       61 ctggatatta gctgtgataa cacgccgcgc cagcaggtta ttgggggaac gcaggctgcg
      121 ttgaatgaat ttgccacgct gctgatggcc gctggatacg aaccggttaa actcggtgtg
      181 agcggtgcct ggcacacgcg tttgatggaa gacggcgttc aggcaatgcg cgattatctt
      241 gccggactgg atatcgcttc gccagagcat caggttctga tgaacgtaac agcaaaaagc
      301 gaggtcgctc cgtcaattat caaagaaaat ctctcattac acttaactca tacggtgaaa
      361 tggactgaat ctttcgatac atttctgaat atgccaaccc cggtagcttt ccttgaaata
      421 agtaataagc cctatttggg gaatatgtta aatgattttg ctggcgtggt tcagcaacga
      481 gttatgcatt gtcgtaaagc atttagtgat gcaaaggtat tcaaatgatt cctgattatt
      541 taacttttat tcgctttcag gataaacgaa atctgatata catttatgct attggactta
      601 ttctgatagg cttttattgg aagaatgcag ggtttacttt tccatcagag gatattggtg
      661 tagttagtgg gattctggcc ctggtgctgt ataattttat ttttgatctc aaggcgtact
      721 gggcttataa atgcgtcacg aagaatatcg atttttcgtg gtttaagaaa aagcagaacc
      781 acaaaataga attatttctt acacaacctc tggtggcagg atttctgtcg ttaatcatgt
      841 tgagtgcaat gagttggggg ctataccagc ttctaccctc gttatatgcg ctgttcctga
      901 tttcgttact tgggccgttg gtcatctttc tgctgtttcg gatgatccgc accagttatg
      961 tcaagcaggt cgctatttca gtagcgaaaa aagtaaaata taaaagtctg actcgctatg
     1021 tgctgctttc ggtgtgcatc tcaacggttg ttaacctgct tactatcagc ccgttgcgta
     1081 acagtgattc ttttgtgaca gaggggcagt ggttaacgtt taaatcgata attgcattgc
     1141 tcattctttg tggcgtagtg ttggcgatta atctgttttt tctgcgcttc tccaagcggt
     1201 acgcttttct gggcaggctt tttttgcagg aaatcgatct gtttttctcc agtgaaaatg
     1261 cgttgtcgac cttttttgcc aagccgcttt ggcttcggtt attcatattg ctggttattg
     1321 aaatgatgtg gattacgctg gtgtcggtat tggcaacgct tgtggaatgg cggatttggt
     1381 ttgaagccta ttttttactc tgctatgtac cgtgcttaat ttactatttt ttccattgtc
     1441 gattcctctg gcataacgat tttatgatgg catgtgacat gtatttccgt tgggggcatt
     1501 ttaataagtg aggaagttac aggaagtgac cagataacgc atatatgttc tctactatct
     1561 tgcgcatttt gattgttgac tgagtaacca gacagttgat gtgcacaatt tcccctcgcc
     1621 ctaacagacg tgggcggggg cacccggtgt gtgcaatata cgaaaaaaaa gcccgtactt
     1681 tcgtacgggc tcttctttaa atatggcggt gagggggacc gcctgaaata aatctgactt
     1741 aatccagggg tgttatataa tttcattata aacaatgtat tacacttttt gttgtccgat
     1801 attatctaat gtattcttag tgaagcgaga gactaatggc atactggatg gcataccaga
     1861 gtgacgggaa ttagcgtatg gcagtattga cggatacgaa agcaagacat atcaaacctg
     1921 atgacaaacc attgccccat gggggaatta ccggactgac ccttcatcct tcttcagtaa
     1981 aggggcgggg gaaatgggtt tttcgttatg taagtccggt gacccaaaaa agacgtaatg
     2041 ctggattggg aacttaccca gaggtcagta ttgctgaagc tgcacgtact gcccggataa
     2101 tgcgagagca acttgctgca ggtgatgatc ctctggagat taaaaaggct gaatctgaga
     2161 aagtcgctat cccaacattt gccgatgcag ccaggcgtgt acatgcagaa ctgtctcctg
     2221 gatgggaaaa tccaaagcat gtaaggcagt ggttatcgac gcttgagaat tacgcgtttc
     2281 ctcaactggg agcaaaaacg ctggattcga ttacggctgc ggacgtggca gaaacactgc
     2341 gtccagtctg gttaaccttg tcagaaacgg caagccgggt taaacagcgc attcatgttg
     2401 ttatgcagtg gggatgggcg cacggttttt gtgtagcaaa tcctgttgat gtggttgacc
     2461 atttgcttcc tcagcagact agaggacgtg atgaacacca acccgcaatg ccctggaggc
     2521 agttaccgct ttttgtggcg accagtgtgt ataccgatga accttataat gttacccgcg
     2581 cactgttatt aatggtgata cttacagcaa ctcgctcggg cgaagccagg ggaatgcgct
     2641 gggctgaaat tgattttcat aagcgggtat ggactatacc tgcagaaaga atgaaagcca
     2701 ggatacagca tcgtgttcct ttatcccggc aggctattta cattctggaa aatatacgtg
     2761 gcctgcatga tgaactggtg ttcccttcac ccagaaagca gcagatcctt tccgatatgg
     2821 tgttgacaag ttttctgcgt aaaaagaaag ccgtcagtga cattccgggg cgagttgcca
     2881 cggcacatgg ttttcgctca acattcaggg actggtgtag cgaacagggg tattcgcggg
     2941 atctggcgga aagggcgctc gctcatacgc tgaaaaataa ggttgaggcg gcatatcatc
     3001 gtactgatct actggagcag cgtgtaccga tgatgcaggc atgggcggat tatgtgatgt
     3061 ctcaaattgt gaataaataa tagtgaaaat gtatattgta cgatcatgat ttgatatgtg
     3121 atcgtagcag tagtaattaa gaaaccgctt ttatgtcaaa tgtattgagc tgataattat
     3181 cagatcgtca ggaaagcgta ttttgattaa caggggatta cttaacaatc ccctttttat
     3241 aaaaaataat ttgcctgttt aactcttcac tcctgctgag actgaattat tcctagtgcg
     3301 tcataaatca gtcttctgcg tagtttattg tt
//
LOCUS       DAZFYC010000258         3266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFYC010000258 DAZFYC010000000
VERSION     DAZFYC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.264"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..199
                     /locus_tag="ACHKW5_005249"
     CDS             <1..199
                     /locus_tag="ACHKW5_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514411.1"
                     /translation="ASYTMHLYCDCRQCTNGKYQSPDFGEYIGTSWAGCAKEARKDGW
                     RISKDKTRAFSPGHKVLRINK"
     gene            196..327
                     /locus_tag="ACHKW5_005250"
                     /pseudo
     CDS             196..327
                     /locus_tag="ACHKW5_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            519..779
                     /locus_tag="ACHKW5_005251"
                     /pseudo
     CDS             519..779
                     /locus_tag="ACHKW5_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1130..2155
                     /locus_tag="ACHKW5_005252"
     CDS             1130..2155
                     /locus_tag="ACHKW5_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010433107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HFO7514412.1"
                     /translation="MSNQNNSPQAKGGRARAEKMSSDERVAVAKNAAKQRWQRIKSGL
                     PSAQFEGVLRINDTELDVAVLTNGKRIISQSSVFKALGRPSRGVRATLDGEIILPAFM
                     DAANLVPYINQDLMGVIKRERYLSNSGVELEGYDASILPLVCDAYLKAREDGALKTNQ
                     IEIAKKAEILVRSLAKVGIIALVDEATGYQEVRPKDALQAYLDKIISKELAAWAKKFP
                     DEFYENIYKLRNWPWTGMSKNRFSVVAHYTRDLVYERLGDSILQELEKKTPKQSNGQR
                     KNKLHQWLTDDVGNPMLAQHLHSLVMMQRLAIANGYGWNRFIKMVDQVMPRKGGTFEL
                     ELNDTSL"
     gene            2178..2300
                     /locus_tag="ACHKW5_005253"
     CDS             2178..2300
                     /locus_tag="ACHKW5_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7514413.1"
                     /translation="MIICITIIVTALVTRKDLCEVRIRTGQTEVAVFTAYESEE"
     gene            2522..2771
                     /gene="rem"
                     /locus_tag="ACHKW5_005254"
                     /pseudo
     CDS             2522..2771
                     /gene="rem"
                     /locus_tag="ACHKW5_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707429.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
     gene            2838..3116
                     /locus_tag="ACHKW5_005255"
     CDS             2838..3116
                     /locus_tag="ACHKW5_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514414.1"
                     /translation="MARNVKYYNSDNSPVLACTHERYSHAFKSELFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3118..>3266
                     /locus_tag="ACHKW5_005256"
     CDS             3118..>3266
                     /locus_tag="ACHKW5_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514415.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKSMRG
                     LPSGVV"
BASE COUNT          938 a          641 c          838 g          849 t
ORIGIN      
        1 cgcaagttac accatgcatc tctactgtga ttgtcgccag tgtacaaatg gtaaatatca
       61 gtcaccagac ttcggtgagt atataggtac gtcatgggct ggctgtgcaa aagaggcgcg
      121 caaggatggc tggcgaataa gcaaagacaa aacgcgtgct ttttcacccg ggcataaagt
      181 tttgaggatt aacaaatgac aacatttacc gacgacgaca agaaactaat taaagaaatc
      241 aaagagcgta tcagcagcct ggaggttcga gacgatattg agcgccgggc ttatgaaata
      301 gcgttagcct cgctggaagc agagccggtg gcatggctgc attcagacaa tggcttaggt
      361 attccggcaa taacgaggag taaaaacatt gctgacagtt atcaaagagc tggtatgttc
      421 agccgctata tatagccaag ccagtgccgg tggtgccaga tgctcgtccg tctttaaata
      481 atggcatagt cggctttgat gaaggctgga acgcctgccg cgccgccatg cttcagtccg
      541 gaaactttcg ggaaaacaag aattcgtcaa ccaataattt tcgggaaatc tcggaaacgt
      601 caaccaactc tccggtaatt cctggtgagg tgttgtccgc aatcctgaag tttgccagag
      661 ttcgtgctga tttcgatgat tttgacggtg acaggcgagg tatcggtgat tgtctggatg
      721 aggccgagca agagcttatc gttaccatta acaaatatgc cagtcagttg gcagtagagc
      781 cggtagtacc tgatgacgtt ctggaacaga cagtagcgtc gcccgcgcca ggtaaccagg
      841 ttagcgaatt aacaatgtgg gttaaacgac tggctcactg cttaaagtac gccaatagct
      901 taagcagctt gcctgataag gcgatggagt atctgaatca gaatgggctg ataagcgcgg
      961 aggatatcat gcggtgaagt gtcctacgta actaattaac ggacagaata tttgctagat
     1021 aggatactga ttaacaatgt taatggttta agtttagcaa gtattggggt tgtttatgct
     1081 tgctaaatga tacatgatat ggaaatacat gccaataaat gaggtcttta tgtcaaatca
     1141 aaataattca ccacaggcaa aaggcggcag ggctagagca gagaagatgt catctgatga
     1201 aagggtggca gtcgcgaaaa atgccgcgaa acagcgttgg caacggatca aaagtggtct
     1261 tccatcagcg cagtttgaag gggttttaag gattaatgat actgaactgg atgttgctgt
     1321 acttactaac gggaaaagaa ttatatccca atcatcggtt tttaaagcct tgggtagacc
     1381 aagtcggggt gtaagagcta ccttggatgg tgagatcata cttcctgctt ttatggatgc
     1441 agccaatctt gttccatata taaatcaaga tcttatgggg gtgatcaaac gagagcggta
     1501 tttgagcaat tctggagttg aacttgaagg gtatgatgcg tccattctcc cattagtctg
     1561 tgatgcatat ttaaaagcta gagaggatgg tgcgttaaaa accaatcaga tagaaattgc
     1621 taaaaaggct gaaattttgg tacgttcatt ggctaaagtt ggcatcatcg ctttagtgga
     1681 tgaagcaaca ggatatcagg aagttaggcc aaaagatgca ttgcaagcat atcttgataa
     1741 aataataagc aaggagcttg ctgcttgggc caagaaattc cctgatgaat tttatgagaa
     1801 tatttataag ttaagaaatt ggccctggac gggcatgagt aagaatcgtt ttagtgtagt
     1861 agcgcattat acgagggatc ttgtttacga gcgattaggt gactcaatac ttcaggagtt
     1921 agaaaagaaa acaccgaaac aatctaatgg gcaaagaaag aacaaattgc atcaatggct
     1981 tactgatgat gttggtaatc caatgttagc gcaacatcta cattctttag ttatgatgca
     2041 acgtttggca attgctaacg gttatggatg gaatcgtttt ataaaaatgg tagatcaagt
     2101 aatgcctcgt aagggaggca catttgaact tgaattgaac gatacttctt tatagtttcg
     2161 gagaggttta aaccctaatc atcatatgca tcactattat agtgacggca ctggtaacga
     2221 ggaaagacct ctgcgaggta cgaatccgaa ccggccagac ggaggtcgct gtcttcacag
     2281 cttacgaatc tgaggagtaa gagtgacctg gcgagggaga aatccctcgc cacctctgat
     2341 gtgtcaggca tcctcaacgc acccgcactt aacccgcttc gtcgggtttt tgttttgatt
     2401 ttcaacgcat ttgaagtttt ggacggtgcc ggaatagaat caaaaatact taagtagcgc
     2461 gcagggataa gagggatggt cccctgaagg ggagtgcgaa ttatccggaa ggactctgat
     2521 tatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt
     2581 tgaaaatgga aatattgtta gtcattaccg cattgtgtgt ttgcaaaaaa aatggggctg
     2641 cgttaattaa ttttgtggat ggtcgagtga cagacggatt tatcttgcgc gacggtgagt
     2701 ttgtcacttc attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt
     2761 cagaagaata aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt
     2821 gccaccggag aaaaccgatg gcacgcaacg taaaatatta caattctgat aattcgcccg
     2881 ttcttgcctg cacgcacgag cggtattctc acgcattcaa gtctgaattg ttccagcacc
     2941 ctccatgcac tgaagagcag gctgaatgga taattcagtg ttaccgcagg cgcggatacg
     3001 aggttaagaa agctcttagt ctcgactacc gtcactggat aatctcagtc agattgcctt
     3061 actccgaacg cccaccgcgt ccgtcccgta cattccagca acgcatctgg aggtaacgtg
     3121 cgggtattac ttcgacctgt tctggtaccg gaactcgggc tggtggtcct taagcccggt
     3181 cgtgaatcat tgccagtttt tcatcgcggc agggtgctgg tggagcctga accgaaaagc
     3241 atgcgcggtc tgccgtccgg agtcgt
//
LOCUS       DAZFYC010000259         3250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFYC010000259 DAZFYC010000000
VERSION     DAZFYC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.265"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..210)
                     /locus_tag="ACHKW5_005257"
                     /pseudo
     CDS             complement(56..210)
                     /locus_tag="ACHKW5_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            725..955
                     /locus_tag="ACHKW5_005258"
     CDS             725..955
                     /locus_tag="ACHKW5_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514416.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1007..2368
                     /locus_tag="ACHKW5_005259"
     CDS             1007..2368
                     /locus_tag="ACHKW5_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO7514417.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIVDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFDR
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     DGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVSVYITDMKITEI
                     PQK"
     gene            2415..2978
                     /locus_tag="ACHKW5_005260"
     CDS             2415..2978
                     /locus_tag="ACHKW5_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO7514418.1"
                     /translation="MHSQLRERIRLLRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVESNSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDIREELPRGTFAYTHVPTMIIRLRA"
     gene            complement(3064..>3250)
                     /locus_tag="ACHKW5_005261"
     CDS             complement(3064..>3250)
                     /locus_tag="ACHKW5_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HFO7514419.1"
                     /translation="SNQSSRKKKNSSSSSSSSSSSSSSSSSSSSRNNHIISNRQMTGL
                     KRQKPTAERPRNERGGA"
BASE COUNT          731 a          917 c          953 g          649 t
ORIGIN      
        1 cagtctccgc cgaacccgct ggcgcggttt cgggctgtcg ctacggtccg atgcgtgaaa
       61 accccgggct tgcagcgacc ggaacggtcg cgccaccctt gtcgcgccgt ggcctgcgct
      121 cccggcatcc cggcaaaagg ccgccgggat gtaaggcgca acgccgtttc gcgcacgcgc
      181 tctccgtcgt tgcggcgggt ccctgctcat tgcctgccgg gtgccacaca cccgtatgag
      241 tccctgtcgc cgggggctga tacagccaca acctgccgca ctgactgtcc agggtaaatg
      301 aacagcccac aaaatttttt acccagaacc cggtaaaaaa ttttgcgttc tgcccaggac
      361 aggtgcgtca ggccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa cgggtgaagt
      421 tcataccaca gggaatacag cagcaggttc ccgcgaaacg tcaaaaccgc ggctcccgaa
      481 actcaacggg tgatgttctt taaaaatcag gatttatttc aggctccggc aatacacatt
      541 tatgtgttct gtcagtcatc cggctggcag gtaatgagaa cgcaggaaca gaaggaggaa
      601 tacaccggag aacgtcagac gcagatatga aaaaagcagg ccggggagga cctgcttaat
      661 ccacaccgca aaaactgttc gagcagtttc tgcggttcca ttcaacagtc ataaggatgc
      721 atttatgaca gtaagtattg tttcaccttc cgcagcggct gtaaagcccc gccgtcatcc
      781 ccgtttcaga cgcgaagaca tccccgcgcc ggaaattgac cccgtgctga aagccttcgg
      841 gcgacacata gcccggagtt ttcaccgggg gcgtggtgta cacatccccg ccatgaaaaa
      901 cacggctttc gggcaggtgc tccgcacact cgaactgaaa cgcgctttta actgatgagg
      961 tgagccgccc cgcgaggggc ggcaacagac ggaaggagaa cattttatga cattagcagc
     1021 aatcaccatg accgccccgg aagctgccag ccctgtgcag atgtaccgcg cgacctactc
     1081 accggatgac aacaaactgc gcctgtatgc cgcgtcacgc ctcgacccgg agacgtataa
     1141 aaaagtccat gatgccggtt tccgctgggc accaaaacag gcgctgtttg tcgcgccagc
     1201 ctggacaccg ggccgggaag atgtgctcct ctcacttgcc ggagagattg tggatgaaga
     1261 cagcacgctt gctgaacgtc aggaagcacg ggcggagcgg tttaccggat acagtgggaa
     1321 gcgggccagt gaatctgcac aggcacttga tgaagtggaa agactggccg cgatgatccc
     1381 gcccggtcag cccattcttg tggggcatca cagtgaacgc cgcgcccgcc gtgatgcgca
     1441 gcgtattgaa aacggcatga aacgtgccgt gatgctcttt gatcgtgcgg aatactggga
     1501 agagcgggcg cggtcggcac tgcttcacgc gaagtataaa gaacgtccgg acgttcgctg
     1561 gcgtcgtatc aaaaaaatcg aagctgattt gcgcaaggct gaaaagacca tcgcgcagtc
     1621 gcagaaatat ctgacgatgt ggcgggctga atcgctggat ctgaatatgg caaaactcat
     1681 cagcagtcat gaccatatca gcgcctgttt cccgctggat acgtatccgc gcccggcaga
     1741 aaaaagccag tatgaaggga gccggtcgtt atggtcggcc ctggatgatg acatcatcac
     1801 cacggagcag gcccgcgaaa ttgcgatccg ctgtcatgaa cggcagattc agcatcagca
     1861 acgctgggtt aaccactatc agaaccgcct gatctatgag cgtgccatgc tggacgaaag
     1921 cggcggcgtg gttacccgga cacaggattt tgagccgggc ggacaggttt tcagccgggg
     1981 cgagtggctg actatcatcc gcgtgaacaa aagcaacggg gcggtgagtt cagtcacaac
     2041 gccgaattac agttttctcg ggtacagcgg cacgatgaaa gtgacgcccg atcgcatcac
     2101 ggactacaaa gcaccatcgg cagaagaagc tgccgtcgcc agccaggccg cgaagcgtcc
     2161 gccggtagtc aactatccgg gggacggttt ccgggaaatg acaaaggcac agtgggccgc
     2221 cctgccccgg gactgtaagg ccgtgcgcag tgtggcagag acagaagacc acggggcata
     2281 ccgctaccgc cgcacaatgg ataataattt ccgcctggtg agtgtgtata tcaccgacat
     2341 gaaaatcacg gaaatcccac agaaataagg tatatccccc cgggaaatcc ccgggggatg
     2401 acggagataa tgacatgcac agtcagttaa gagaacgtat ccggctgctg cgcgcaaggc
     2461 tggataacgc cgcgccggtt gctgaaatcc gggctgaatc tcagcttttt gtgacacctg
     2521 ccccggtctg tgatcgcctg gtgacgctgg cggaaatcag caaccgtgat cacattctgg
     2581 aaccctctgc cggcaccggg gccatactgc gggcgattcg ggataccgcg ccggaggcca
     2641 tgtgtgatgc ggtggagagc aacagcgggc tggtccggta tctgcgggaa aattttaacg
     2701 gtgtcagggt acagtgtggc gacttcatgg aatggcagcc ggtgcagtat tacagccgga
     2761 ttataatgaa tccgccgttc agccacgggc aggatatccg gcatatcctg cgagcctttt
     2821 ccctgctgcg tccgggtggc gtgctggttg ccgtctgtct caacgggcca cgccagcagg
     2881 agaagctgtt accgttttct gacatccgcg aggagctgcc gcgcggcacg tttgcttata
     2941 cccatgttcc gacgatgatt attcgtctgc gcgcctgatg acacagggca ggcgggagga
     3001 taaatgcccg tctgcccgtg caggaaaaag accgcgcgcc ggtggcgctg aaaggggccg
     3061 cgctcacgcg ccgccccttt cattgcgcgg acgttctgct gtgggcttct gtcttttcag
     3121 acctgtcatt tgtctgttac tgatgatgtg gttgttcctg ctactgctac tgctactgct
     3181 actgctactg ctactgctac tgctactgct actgctactg ttcttctttt ttctgctgct
     3241 ctgattactg
//
LOCUS       DAZFYC010000260         3243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFYC010000260 DAZFYC010000000
VERSION     DAZFYC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.266"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..593
                     /locus_tag="ACHKW5_005262"
     CDS             <1..593
                     /locus_tag="ACHKW5_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFO7514420.1"
                     /translation="YSLIKTEQTGKLLNRKITTTPTPAKLLSYRNADLIKENYITEKV
                     LSIFNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVMDWYNPQKNAFGIMMGTPRR
                     SADIAKEESRNALNFMIMEKDTFNEKILNSNDNLQKKFGTTENSSWVSASVGSLLDKG
                     AKVYPDISCSLRLGEPFIITLPETVRLDVDIHPLKK"
     gene            complement(606..1304)
                     /locus_tag="ACHKW5_005263"
     CDS             complement(606..1304)
                     /locus_tag="ACHKW5_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514421.1"
                     /translation="MNEIRSNPVKFLEEHLILNPQRYAHHDETSLITVNITREDGLLR
                     LKETEDDMAGSDYILFTGMRDTDSPDRFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQSYSMIHMRPRDSNDFVDEFTPALYANTQEILLER
                     DIQRTLANTFPNEHPEALILVPSAEKFIVDGNSIQLIGIGNELGEFDFYRQIYPVTGG
                     EHLVEALMWTHLPA"
     gene            complement(1505..2086)
                     /locus_tag="ACHKW5_005264"
     CDS             complement(1505..2086)
                     /locus_tag="ACHKW5_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO7514422.1"
                     /translation="MPLTSDINSSSFHLGMEVLRAQVAATGRGEFTMGGETVRIEYSP
                     TDGRFLASDGTGGLFTELLLLGFNNGPQALGERMLSILSGSDAGETQSQVTPQDKIYQ
                     CKFSVNTESLQCPSDATRCPIILETPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSV
                     HPLTRAPITPSMIVKPEECKYDPARGSFIIKDS"
     gene            complement(2155..2292)
                     /locus_tag="ACHKW5_005265"
                     /pseudo
     CDS             complement(2155..2292)
                     /locus_tag="ACHKW5_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            complement(2513..2662)
                     /locus_tag="ACHKW5_005266"
                     /pseudo
     CDS             complement(2513..2662)
                     /locus_tag="ACHKW5_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2980..>3243)
                     /locus_tag="ACHKW5_005267"
     CDS             complement(2980..>3243)
                     /locus_tag="ACHKW5_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514423.1"
                     /translation="ILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENGII
                     PEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          974 a          793 c          564 g          912 t
ORIGIN      
        1 catattctct aataaaaaca gagcaaacag gaaaactgtt aaacagaaaa ataaccacca
       61 ctcccacacc agcgaaacta ttatcatatc gaaatgcaga tttaataaaa gaaaactaca
      121 ttacagaaaa agttttatct attttcaata taaaacggga tttcgttgct gtaagaatcc
      181 aaagcaatca gtttactgat ttaaaaaaca agacaattca gggtcataaa gacactgtcg
      241 caaaagtcat ggactggtat aacccacaaa aaaacgcatt tggtatcatg atgggcacac
      301 caagacgttc tgctgatata gcaaaggaag aatccagaaa cgctctaaat ttcatgataa
      361 tggaaaagga tacatttaat gaaaagatac ttaacagcaa tgataattta caaaaaaaat
      421 tcggcacaac agaaaatagt agttgggtat cggcttctgt tggttctctt ctggacaaag
      481 gagccaaagt atatccagat atatcatgct ctctgagact gggagaacca ttcattatta
      541 cacttccaga aacagtcagg ctggatgttg atatccaccc actcaaaaaa tgaacctgaa
      601 cgttttcatg caggcaaatg cgtccacatc aaagcttcaa ccaggtgctc tcctcctgtc
      661 acaggataaa tttgtctgta aaaatcaaac tctcccaact cattaccaat accaatcagt
      721 tgaatgctgt tgccatctac aataaatttt tccgctgacg gaaccaaaat taaggcttcc
      781 ggatgttcat taggaaaggt gttagcaaga gtacgctgaa tatccctctc cagcaaaatc
      841 tcctgagtat tagcgtaaag cgctggcgta aactcatcga cgaaatcgtt actatccctt
      901 gggcgcatat gtatcattga atacgactgt tctccagatt ctgataatcc atggcgaaca
      961 ataagaacac tacagccgct ttgctgattg gttagccata acggtgaact ttgctggata
     1021 gtttgagaaa tatcatttcg ccggaggctt atacactgat tactcaagct tacaactgca
     1081 ggtgcatcaa agcggtccgg actatcagta tcacgcatac ctgtaaacag aatataatca
     1141 ctaccagcca tatcatcttc cgtttctttt agtcttaaca agccatcctc tcttgtaata
     1201 ttaactgtaa ttaaagatgt ctcatcatga tgggcatagc gctgtggatt aagaattaaa
     1261 tgctcttcca gaaattttac agggttacta cgtatttcat tcattataca ccgaagctct
     1321 gaacgatgcg gcaccagaaa gtgcccggta ttttgtgaaa taatactact taccttgatt
     1381 gttttctctc cagctcatga aaaataactc gcgtattata ttgttttacc aaccacaggc
     1441 cccgaatgcc tgtggtgttt ttatataaat tatcggaagt taatattgtt tcatttgaaa
     1501 catcttaact atcttttata ataaagcttc cccttgcagg gtcatattta cactcttctg
     1561 gtttaacaat cattgatggg gttattggag ctcgtgtcag agggtgaact gaaccgtcat
     1621 taaccacgcg agaaagtgca tcaacatcaa ataaagtgca tactgctgaa ctatctgaat
     1681 ttttgacaaa taccccttct tctggtgttt ccagtataat tgggcatcga gttgcatcag
     1741 agggacactg gaggctctct gtattaacag aaaacttaca ttgatatatt ttatcctgag
     1801 gagtcacttg cgattgtgtt tcacctgcat ctgatcctga aagaatactt aacattctct
     1861 cgccaagagc ttgaggccca ttattgaacc ctaaaagcaa taattcagta aataatcccc
     1921 cagtgccatc gctggccaga aagcgcccat ctgttgggct atattcaatt ctgacagttt
     1981 caccacccat tgtaaattct ccacgcccag tggctgcaac ttgagcacga agaacctcca
     2041 ttccaagatg gaacgaagat gaattaatat ctgaggttaa tggcataata aagctccctg
     2101 ttttaatttg aactccagac ttaaaggtcc acaacaaatg tcatccacaa atttttccct
     2161 cgtcccatca gcacaccgaa tttgcagcgg cctcaccacg cactgtatcg gctttttatc
     2221 cgcacccagt atcaccacct gcgtgattac cctgtcctgc tccggaataa tgccctattc
     2281 gcttgttctc agaaattgcc agcaacctta cactacttct tttgagccca tttttcgcca
     2341 gccctgaata caggaaagtg ttaactgttt ttatgtttac cctcatctca ctggcgagat
     2401 gggacggacg caactgacgg tagatatatc caaacatcac caccatctct gacatcgtca
     2461 gtgcccgtgc ttttttgttc caggtccaca atctgttacg cacagcatta tgtatatcat
     2521 gtcccccttt caacgcttcc agatccagct gaataaaacg gcgtccctga aacatgctgc
     2581 gcattaaatg gcccaactga ctatcacaga ttattaacca ggattcttta ctatccagaa
     2641 taaccctgcg attttccggc ataaaaaatt cttgagccag aattacacgg catcctttca
     2701 gtaacaaacg caaaccatat tccagataat aattacgcgt cgcaagaatg agcttcatcg
     2761 ccctacttcc ctgaactaca gtagtgttcc atgcctcggt cgcaatcaga atttatgatt
     2821 ccccctccag ggaacaagat ggacactccc ctgctcctta aaggcatcct tcactccatc
     2881 gtattaacat gtttattact tcacactcta ataacaaaca tcccccatac atgccaacaa
     2941 aaaccggagc cggcctccgg ttttttgtga acccgtcggc tatttcatcc cgccaatatt
     3001 ttcccacgtc ccgtcagcac gcaggatttg cagcggtctt accacacact gtatctgctt
     3061 tttatctgca tccagtatca ccacctgtgt gattaccctg tcctgctccg gaataatacc
     3121 attctcatcg gactccagga tgtctgccgg ccccagtcgc agttgtgctg taagcgactg
     3181 cacgtgttca cggccatcat gctttccgca accacacaga cgctgcataa gtttttttag
     3241 tat
//
LOCUS       DAZFYC010000261         3231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFYC010000261 DAZFYC010000000
VERSION     DAZFYC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.267"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHKW5_005268"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHKW5_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO7514424.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHKW5_005269"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHKW5_005269"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO7514425.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="ACHKW5_005270"
     CDS             1253..1522
                     /locus_tag="ACHKW5_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO7514426.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="ACHKW5_005271"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="ACHKW5_005271"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO7514427.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHKW5_005272"
     CDS             2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHKW5_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO7514428.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          813 a          776 c          794 g          848 t
ORIGIN      
        1 aggcccagtc ggaagactgg gcctttttgc gctggtgcag aaaaaggtcg gatgcgacac
       61 tggctgcgtc tgatccagcc tactcttgct tatccgttct gcttttgcat ccactccacc
      121 acaaaatcag ccagcccctc aacatcatta atatccagta atgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc aggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaactgtag atccagctct
      361 tcttcgtctg gtgtttccgt catcaaggcc catcgttgct ggctggcaac gatggtttgt
      421 gccgcgccag ccttgcgcag ctcatagcta tctttgcctg gcttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcagt
      541 tgtttcaaaa gcgtagtttt tccggtacca ctccacgcgg caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgttaata aatgcatctt
      661 tattatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc attacccggc
      721 gttctcctgc ttgcagatat tccagtaata aaggctcaat tgcgcggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      841 gattaagccg ggctgctaaa tcctcgggaa tataaggagt atcgcacgga caaaacaaaa
      901 accactcgcc tgcttcctgc tgcattactg aaagcattcc tgccagaggg cctgggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttgcctg atagatttcc tgatggcgat
     1021 tggcattaac cacgacgtga gagagctgcg tcataagcgc gtcagcgaca tgttgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcctgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1321 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca tctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagacttc aaaacggcgc tgttacgcgc
     1501 tcgcggcgta attaaagagt aaaagcttgt aagcggcgcc accaaaatca tcgtgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg atgctctatt tgagcaaggg atccgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 atcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1801 tcgaagaaca tcaatttgcg ttgcagctgg taaatgatga agttccggtc gcagcacctg
     1861 tggcctttaa cggtcagact ttattgaatc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2041 gtctgaatga atatctcatt gagcctcgca agctgtttga ggacgctaca ctgatacctt
     2101 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gatcgccgcc gttacagcac
     2161 actggcggga agacttcacc gttctgcggc tacatggaga ctgccacgcc gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagctattc
     2281 aggatttgtg gatgttgctc aatggcgata aagccgagca gtggatgcaa ctggaaacta
     2341 ttattgaagc ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttt ccgtggttaa ccggggaaga ttactggctg cgacagacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttgcaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccggtagctg gcgcgccgca agtgctggag tttttctctt tcttctgccc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aatgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg ggtggtgacc tgggcaaaga tctgactcag
     2881 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tgactgttcc gctgtttgaa
     2941 ggcgtacaga aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt gaaatctctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggtgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggcatggata ccagcaatat ggatgttttt
     3181 gttcagcagt atgctgatac agtgaaatat ctgtccgaga aaaaataatt a
//
LOCUS       DAZFYC010000262         3193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFYC010000262 DAZFYC010000000
VERSION     DAZFYC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.268"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            36..230
                     /locus_tag="ACHKW5_005273"
     CDS             36..230
                     /locus_tag="ACHKW5_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO7514429.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            223..657
                     /locus_tag="ACHKW5_005274"
     CDS             223..657
                     /locus_tag="ACHKW5_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7514430.1"
                     /translation="MRDIRQVLECWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGGTFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1164..2111
                     /gene="stxA1a"
                     /locus_tag="ACHKW5_005275"
     CDS             1164..2111
                     /gene="stxA1a"
                     /locus_tag="ACHKW5_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO7514431.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAP
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2121..2390
                     /gene="stxB1a"
                     /locus_tag="ACHKW5_005276"
     CDS             2121..2390
                     /gene="stxB1a"
                     /locus_tag="ACHKW5_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO7514432.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2901..>3193
                     /locus_tag="ACHKW5_005277"
     CDS             2901..>3193
                     /locus_tag="ACHKW5_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7514433.1"
                     /translation="MAFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKLTGGEGAVISATSDPGYYFVVVLAGQSNGMSYGEG"
BASE COUNT          849 a          616 c          827 g          901 t
ORIGIN      
        1 cttaaagacc tgcgaaacag cagaagtgag gccgcatgac gttcacagta aaaaccattc
       61 ctgacatgct cgttgaggca tatgaaaatc agaccgaggt agccagaata ctgaactgta
      121 gtcgcaacac ggtcagaaaa tacactggcg ataaagaagg aaaaagacac gctattgtca
      181 acggtgttct tatggttcat cgcggatggg gtaaagatac tgatgcgtga tatccggcag
      241 gttcttgagt gctggggggc atgggcggca aataaccatg aggatgtgac ctggtcaccc
      301 attgccgccg gatttaaggg actgatcccc gaaaaagtaa aatcacgccc gcagtgctgt
      361 gacgatgatg cgatggtgat atgcgggtgt atagcccgcc tttaccggaa caatcgcgat
      421 ctgcatgact tgctggttga ttattacgtg ctggggggga cgttcatggc gctggcacgg
      481 aaacatgggt gctctgacac ctgtataggt aaacgccttc acaaagcgga ggggattgtt
      541 gaaggcatgc tgatgatgct gggagtgagg cttgagatgg atcggtatgt tgagcgtgaa
      601 ttgccgggag ggagaacctc tgtattttat cagcgaaaaa atagtttacg atcgtaaaaa
      661 tctgcatatc atgataagag tggttacatt gccacgctgc ttaacccgcc gatgcgcggg
      721 tttttttgta cccagaatcc tgtgagctat acggaaagta cacagaaagg aaggtgcgac
      781 cacaattaat aacaaaatct taaaaattgc acatggcact attagttttc taaatattgt
      841 gtattttttg tattgcagga tgaccctgta acgaagtttg cgtaacagca ttttgctcaa
      901 cgagtttgcc agcctccccc agtggctggc ttttttatgt ccgtaacatc ctgtgtatca
      961 ataaatgttg ttgtctacgt acgtcaagta gtcgcatgag atctgaccag atatgttaag
     1021 gttgcagctc tctttgaata taattatcat tttcattacg ttattgttac gtttatccgg
     1081 tgcgccgtaa aacgccgtcc ttcagggggt ggaggatgtc aagaatatag ttatcgtatg
     1141 gtgctcaagg agtattgtgt aatatgaaaa taattatttt tagagtgcta acttttttct
     1201 ttgttatctt ttcagttaat gtggttgcga aggaatttac cttagacttc tcgactgcaa
     1261 agacgtatgt agattcgctg aatgtcattc gctctgcaat aggtactcca ttacagacta
     1321 tttcatcagg aggtacgtct ttactgatga ttgatagtgg cacaggggat aatttgtttg
     1381 cagttgatgt cagagggata gatccagagg aagggcggtt taataatcta cggcttattg
     1441 ttgaacgaaa taatttatat gtgacaggat ttgttaacag gacaaataat gttttttatc
     1501 gctttgctga tttttcacat gttacctttc caggtacaac agcggttaca ttgtctggtg
     1561 acagtagcta taccacgtta cagcgtgttg cagggatcag tcgtacgggg atgcagataa
     1621 atcgccattc gttgactact tcttatctgg atttaatgtc gcatagtgga acctcactga
     1681 cgcagtctgt ggcaagagcg atgttacggt ttgttactgt gacagctgaa gctttacgtt
     1741 ttcggcaaat acagagggga tttcgtacaa cactggatga tctcagtggg cgttcttatg
     1801 taatgactgc tgaagatgtt gatcttacat tgaactgggg aaggttgagt agtgtcctgc
     1861 ctgattatca tggacaagac tctgttcgtg taggaagaat ttcttttgga agcattaatg
     1921 caattctggg aagcgtggca ttaatactga attgtcatca tcatgcatcg cgagttgcca
     1981 gaatggcacc tgatgagttt ccttctatgt gtccggcaga tggaagagtc cgtgggatta
     2041 cgcacaataa aatattgtgg gattcatcca ctctgggggc aattctgatg cgcagaacta
     2101 ttagcagttg agggggtaaa atgaaaaaaa cattattaat agctgcatcg ctttcatttt
     2161 tttcagcaag tgcgctggcg acgcctgatt gtgtaactgg aaaggtggag tatacaaaat
     2221 ataatgatga cgataccttt acagttaaag tgggtgataa agaattattt accaacagat
     2281 ggaatcttca gtctcttctt ctcagtgcgc aaattacggg gatgactgta accattaaaa
     2341 ctaatgcctg tcataatgga gggggattca gcgaagttat ttttcgttga cttagaatag
     2401 ctcagtgaaa atagcaggcg gagattcata aatgttaaat acatctcaat tcagtcagtt
     2461 gttgccggtc tgataataga tgtgttagaa aatttctgca tggtgaatcc ccctgtgcgg
     2521 aggggcgact ggtgaacggt atgatctctt tgatgatcgt aagcgagaat acgcgggttt
     2581 ggtggcacca ggccgaactc accgggaggc acccggcacc atgcaatggc acatagcgcc
     2641 actctccagc ccctctccgg aggggctttc ttatggacaa aaaaagcccg cgcagggaga
     2701 cgctggcggc aaggaataaa caacaaaacg tgaagtaata tttcagctgg cgaataatat
     2761 ccgacagtaa tcactctgcg caatagcgcg gcctttttcg tattgcgggc tgttgtctct
     2821 cttctgccat tgtcctgtaa cttccggatt tcagcccgct catcatttta ctcacaatat
     2881 tatcccggcc gggaggattc atggcattta aacactacga tgtggtcagg gcggcatcgc
     2941 cgtcagacct tgctgatgca cttgcgcaaa aaattcgtga aggatggcaa ccatacggtg
     3001 ggccgttttc ttcgtatacg gatgatggcg cagcacttat tcaggcgatt gtcgcagaag
     3061 gtgatgtgag cacacctgtt gtggtgaagc tgacaggtgg agaaggtgca gtaatcagcg
     3121 ccaccagcga ccccgggtat tactttgttg tggttctggc agggcagtca aacggcatgt
     3181 cgtatggtga agg
//
LOCUS       DAZFYC010000263         3095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFYC010000263 DAZFYC010000000
VERSION     DAZFYC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.269"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..1984)
                     /locus_tag="ACHKW5_005278"
                     /pseudo
     CDS             complement(77..1984)
                     /locus_tag="ACHKW5_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(2004..2465)
                     /locus_tag="ACHKW5_005279"
     CDS             complement(2004..2465)
                     /locus_tag="ACHKW5_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO7514434.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(2493..>3095)
                     /locus_tag="ACHKW5_005280"
     CDS             complement(2493..>3095)
                     /locus_tag="ACHKW5_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514435.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRSTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT          585 a          902 c          828 g          780 t
ORIGIN      
        1 aataaggaat atctcaattt ttaaacataa aatgcgaatt atttagtaca aaaaaccggc
       61 gttacatgat ctttccctgg ctccacgtct ggtcgttatc ggaccagcgg atgatccggt
      121 ccgcagcgtc gtactcataa aacaggtggt agtccagccg cgcatccgct tcggtcagcc
      181 gcccccgcgc atcctgttcg taggtcgcca gcgtctgtat gccgttatcc gtgcggtgaa
      241 tggctttcag gtagccgctg gcgtggtacg tcagtttaag ccggatcccg tcggtgtgac
      301 tgacagccgt gatccggtac tggctgtcgc gctcaaacgt gatgcggttg ctggcggtgt
      361 cgctcatccc gcccagcatg tacatccggt cattggggct gtgtgccagg cggtcatcgc
      421 tggtatcgtc agtcaccggg tggcgcagac gcattcccgg aaaggcgtca taaaagaagc
      481 ggctgctcag tgcatccctg tcgaacagct ccagataatc atcgcggcgg cgcagaatgt
      541 actgggcgtg atacgggtta accgtcgtat cactgtcacc gtcgaaggca aatctcagcg
      601 tggctccctg gctgatgata tccacccggt ttccctgttc acgcacccag gcgtattcgc
      661 tccaggagtc gctccagccc acgcccgtca ggccatgcag cggcagtaca gaactgtgga
      721 aacgggtgaa ggagaggggg agggtctggc ccagggtgaa gtcggtacgt tcgtcgcagt
      781 acgcgccggt taccggatcg acaggatctg ttacccatct gatgacttta ttggcaatac
      841 tttttaacga cttacctgtt ttccccagca acgcgccgag ctttgcgccc agcagcttcc
      901 cgcttttccc cagcaggttt cccagtttgc ccagtttcgc cagattcccc agttttccca
      961 gcagtcctgc tggcggcaga agatagctgg ctatcgttat tcctcccagg atcattctct
     1021 gccatggcgg gaactccggc tggatatcca gataggtgac ctgctcaccc ccaataaaga
     1081 cgtccggcga accttccacg atcgccgcac tgcactccag tttgtcgccc ttgcgcgcgg
     1141 caggctgatt gttgataaag acgttgctac tgccctgggc tatcctcggt ggtttgttct
     1201 ccttgtcgca gagcgccgtg tcctcaccgg cttccgggaa atccacctct tttttgattt
     1261 cgccacgccc gcccaccatg gtggcaaact ccattaatac cggaccggcg ggaaacagaa
     1321 gcgatgcgcc ggcgctgacg cgatccataa aaccggcatt gctttccgtc agggtgataa
     1381 cagcgttccc ccaggccttc gccagtggta ctgctttttc cgctgccgat tcaagcaggt
     1441 cagtggcaat atcaccccat gagggttccc cctgtgggat ttcaggtatt gctccaggtg
     1501 ggggaggagg gggaagggtg acggcggccc ttgcagcggg ctttccgtta atgtttacat
     1561 tttcagaacc ggtattgatg gcgccgtccg tgtcacaaaa gctgtttatc catgattcaa
     1621 gctggttctg tttctcctcc acataatcac tcgccttata gctggcatac atcagcgcag
     1681 gcaccagaac cagcgcataa gggccggtgg cgcaggcggc cataatcagg caaccttctg
     1741 cgatggtcac tatcgccccc agcacggccc ctgccagtgc ccccagaaaa cttttatgct
     1801 gaatttcatc gcccagcctg gcggcaggtg ggccggactt ctttgctgcc agcatctgtg
     1861 caatggcttt gtcgttggct gcccgtttca tctggctggc gaccatcgcc tgctgtgaca
     1921 gcattcgcat tgccagcgta ccgcctgcgg tcgctccctg tggcccgcct ggtccttcac
     1981 tcatgtgtta tccctcgttt atgtcagcgc ggctggtcct tttggatgtc attctccggt
     2041 ggcaacgggg tgaagctctt tatcacctcc agcagcatcg tcttttgact gttcttcagg
     2101 gataaggagg tgctgaaagt gaaggtcagc agcgtatctc cacgccgctg gaatacggct
     2161 atctggctaa tccgcccctg attcccctca tcttccggac cgttccagga ataatccagc
     2221 agccaggccg gggcctctgc cagagtgatt tcccggcagg catgttcggt gtacccatca
     2281 agtttgccct ggtacagcgc cagcgtttcc tggtacagct ctggtgtcag ggttccctgt
     2341 ggaacggctt cgcgggtaat gaccaggttg atgccactgt tatctgtgga caccaggacc
     2401 tgcattgact gatcctgcca ttgagacggc agttgaatgt ggccttcatt aaaagtgaac
     2461 tgcataaaat actccctgat aatctgagtg aatcagttga tgaaaacaga accgcccacg
     2521 atggtgatgc catccttcga cagcctgatt accgtcgatt ccccgacctg tagctgaatg
     2581 ccatccggcg tcagcgcaat tttgctttcc tccacggcca gcaaaatttc cttaatcgcc
     2641 gtataagcct gtataccgtc gccttcgcca ccattgttta cagcgtcaac atgcaccgaa
     2701 ccggcaatcg tgcttctgtc ctgaaagaca tcctcagata aagacccttt cagtactccg
     2761 gtgatcttgt ggcctttttc caccgtaaga aggcggttac cttcctggat cgtgaccgtc
     2821 tggtcatttt ttacggtttc gcggtgatcg tgttttacgg tggtggtacg gtcgttgagc
     2881 acttcggtat ccatgttctt ctgcgcgtgg atgtagacct gttctctccc tgtcgcatca
     2941 tcaaacttca gctcattaaa tccgctgccc atgtacgttt tggagcggat ggtcatctgc
     3001 gtctttgttc ccggcaggct gccgggggtg cggttttcct ggtggtaggt gcgccccata
     3061 atgatcggct ggtccggatc gccgttgagg aagtc
//
LOCUS       DAZFYC010000264         3004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFYC010000264 DAZFYC010000000
VERSION     DAZFYC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.270"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..237
                     /locus_tag="ACHKW5_005281"
     CDS             <1..237
                     /locus_tag="ACHKW5_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514436.1"
                     /translation="CDGREYISTEEFNAWMEENNLQYVLCFRDEGCADLDACDADISA
                     WEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAEA"
     gene            365..583
                     /locus_tag="ACHKW5_005282"
     CDS             365..583
                     /locus_tag="ACHKW5_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO7514437.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            585..1142
                     /locus_tag="ACHKW5_005283"
     CDS             585..1142
                     /locus_tag="ACHKW5_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7514438.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1433..1588
                     /locus_tag="ACHKW5_005284"
     CDS             1433..1588
                     /locus_tag="ACHKW5_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7514439.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1708..2052
                     /locus_tag="ACHKW5_005285"
     CDS             1708..2052
                     /locus_tag="ACHKW5_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO7514440.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            2174..2446
                     /locus_tag="ACHKW5_005286"
     CDS             2174..2446
                     /locus_tag="ACHKW5_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514441.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2448..>3004
                     /locus_tag="ACHKW5_005287"
     CDS             2448..>3004
                     /locus_tag="ACHKW5_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514442.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          796 a          666 c          813 g          729 t
ORIGIN      
        1 tgtgatggtc gggaatatat ttcaacggaa gagtttaacg cctggatgga ggaaaataat
       61 cttcaatacg tcctctgctt cagagatgaa ggatgtgctg accttgatgc gtgtgatgct
      121 gatatttctg catgggaacc ggaacgacca gagggcgatg gctggtttat tggttccatt
      181 catgatacgg aagatggccc ggtttgtgtc tggttgcgaa ataaggctga agcataaagg
      241 cgataaacca actaacaact aaatactgaa gatttaaatc agaaacgatt tttattaaat
      301 ccttaaccgg agggattcct gcaccctcag aacatcagga ggccgcccga aagggcggta
      361 agaaatgaaa cattatttag aaaaaaatta cccacgaaag agcagaacaa cagagtttct
      421 gtttttcatt ctgtttatag tgttgatgat accgatatcc ccgctattac tggtctggat
      481 aattggaagg acatttgaac cagttattga gctatatacc gatgtgacat gggaatcatt
      541 cagcgcactg cacaataaaa ttaatccgta taaggaaaac tgatatgagc actattacca
      601 gagaacgcgc ggagattaaa tcatacatca caggcttcct gagcgactcg gcgcacgata
      661 acaagtcttc agacagcctg ctggctaatg tgtttcgtat cgcgctggca tcactggaag
      721 cagagccgat agcaatggta gtgcctgatg aaatggattt gcttacctgc catctcgacg
      781 gtgtaactaa aacatatgct gatggctgga acgcctgccg cgtcgccatg cttcaggccg
      841 gaaactttcg ggaaaataag aattcgtcaa ccaacaattt tcgggaaatc tcggaaacgt
      901 caaccagatc tccgataact ctggatggct ggataagctg tactgagcga atgcctgaaa
      961 agagccagaa cgtgcttatt tcgatgaata tcgatagcga ggctgggcca ttaatatatt
     1021 ccgcacgcta tctcggaggc acgttccggc gcggaggtat agcagttagt ccgggtaatg
     1081 atcttaggca agcaacccac tggatgtcgc taccagaacc gccgcaggag gtgaatcaat
     1141 gacctggcct gaagcattca caacggtagg aattgcgatg gcggtggcgc tggtggtgta
     1201 ttcgatttgc cgctggggat aaaaacggtt tgcgggaaaa ggatagttaa gtagaattgc
     1261 agcgggtgct tgaggctatc tgcctcgggc atgaacacca acggcagata gagaaaagcc
     1321 ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggctc aatccatgct
     1381 gaacacatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca
     1441 gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag tgacggcact
     1501 ggtaacgagg aaagacctct gcgaggtgcg aatccgaacc ggtcagacgg aggtcgctgt
     1561 cttcgtagac tacgaatctg agaagtaaga gaccaggcgg gggagtaatc tcccgccacc
     1621 tctgatgtgt caggcatcct caacgcaccc gcgctttacc atactgaaaa tgctgtttga
     1681 atgttcatct ctgaaagagg actatgaatg aaaaaggtat tgattgcagc acttatttcc
     1741 ggtgtgtctt ttggcgcttt tgcacagcag ggtggtttcc aggggccaga agcagagcgt
     1801 tcaacagtag cgcaggcaaa agaactgaag gatgatgcat gggttatcct tgaagggagc
     1861 atcgttaaaa aagtgggtga tgaacgttat gagtttcgtg acaatagcgg gacaattgtc
     1921 acggatattg atgacagcgt atgggccggg cagaatgttt ctccgaaaga caaagtaaga
     1981 attgagggtg aaattgataa agacctgagc agtgttgaag ttgatgtaaa ggcactgaaa
     2041 ttattaaagt aaccgcccct gcttgttaag cccgtcttac tgacgggttt tctgtttgta
     2101 cattccggcg tattgcctta caattcgcgc agtcagcctg aacaactgac acctgctgtc
     2161 accggagaat ccgatgacac aacacataaa atcccacaat tctgaagccg acccggaaat
     2221 taagcagggg aggcgttttc gtgcgcctca gtatggctgg tttcactatc tgttctgtac
     2281 gatcgatgag gcagatatgc ttcaagaggc gtatctgcgt cgcggtgtcc gtgtggagcg
     2341 gagtctgaac gctgatcgtc tgacctggac cgtttctgta tatcttcctg ttcgtgcaca
     2401 tctgccacgg acacatgcct gctaccgtca gcgcgtctgg aggtaatgtg cgggtattac
     2461 ttcgacctgt tctggttccg gaactcgggc tggtggtcct taagcccggt cgtgaatcat
     2521 tgccagtttt tcatcgcggc agggtgctgg tggagccgga accgaaaaac atgcgggcgc
     2581 tgccatctgg agcggttcct gctgttcgcc agccgctggc ggaagataaa tcactgctgc
     2641 catttttcag cgatgagcgg gtgattcgtg cagctggcgg cgctggtgca ctgtctgact
     2701 ggttattacg tcacgtgaaa tcctgccagt ggccacacgg cgattatcat cacagcgaaa
     2761 ccgttattca cagttacggt gctggcgcaa tggtgttgtg ctggcactgc gacaaccagc
     2821 tgcgcgacca gacctccgaa tcacttgagc aacttactca acaaaatctg acagcctgga
     2881 tgattgacgt catacgccat gtaatgaatg gcacgcagga gcgggaatta tcgctggctg
     2941 aattatcctg gtgggcagtc tgcaatcagg tggtggacgc attacctgag gcagtatcgc
     3001 gtcg
//
LOCUS       DAZFYC010000265         2987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFYC010000265 DAZFYC010000000
VERSION     DAZFYC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.271"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..91)
                     /locus_tag="ACHKW5_005288"
     CDS             complement(<1..91)
                     /locus_tag="ACHKW5_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7514443.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYT"
     gene            complement(261..644)
                     /locus_tag="ACHKW5_005289"
     CDS             complement(261..644)
                     /locus_tag="ACHKW5_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514444.1"
                     /translation="MRKKQYDTPLAERLDTISQQHHLSGSDLARIAGVGRSSVNAWKK
                     RGTISKDSAAKIAEATNVSLSWLLTGKEDTNREALDDDEKALLDVYRNLPPVERRNML
                     AAFQMRLQKLTEFYSEYVDPITRQK"
     gene            754..1029
                     /locus_tag="ACHKW5_005290"
     CDS             754..1029
                     /locus_tag="ACHKW5_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7514445.1"
                     /translation="MTALDKAIKIAGGIRPLGRAIGAWPSQIHKWANEYNGRVPTGER
                     VRQIYIATGVTPHELRPDLYPNPTDGLPVGCKANTQNTPELIHENQA"
     gene            1013..1435
                     /gene="ydaT"
                     /locus_tag="ACHKW5_005291"
     CDS             1013..1435
                     /gene="ydaT"
                     /locus_tag="ACHKW5_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7514446.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLLPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAYFSGGGSPGDSVVVH"
     gene            1513..2301
                     /locus_tag="ACHKW5_005292"
     CDS             1513..2301
                     /locus_tag="ACHKW5_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514447.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKCNAASVTQTDQKALPEEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIDLPDWLPETLWHEWVRFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            2308..>2987
                     /locus_tag="ACHKW5_005293"
     CDS             2308..>2987
                     /locus_tag="ACHKW5_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7514448.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLTEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGYT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASLRSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGG"
BASE COUNT          789 a          666 c          802 g          730 t
ORIGIN      
        1 tggtatacgt catcgcggtg aacgtaccgt cctggttggg gaacacgcca cataccagag
       61 attcgttgtt gccaagattg agcgtatcca tgttgacctc atttgccctt aacgccgggg
      121 gggcggaact aagacctgtc gcaccgttgt gctttgatgg gatatattgt tctcttcaaa
      181 tgaacatatg tcaacattgt gaatgcgcaa gaggcaaaaa aaaaccgcct tccggcggta
      241 ttgttttgta aaataaagaa ttatttttgt cgcgttattg ggtcaacgta ttctgagtaa
      301 aattcggtca gtttttgaag gcgcatttga aaagctgcca gcatatttct acgctctaca
      361 ggtggcaggt ttctgtaaac atcaagcagg gctttctcat catcatcaag cgcctctctg
      421 tttgtatctt cttttcctgt cagtagccag gaaagggaaa catttgtcgc ttctgcaatt
      481 ttggctgcgg aatctttact gattgtccct ctttttttcc aggcgttgac tgatgagcgt
      541 cctacaccag cgatgcgcgc taaatctgaa ccgcttaaat gatgctgttg cgagatcgta
      601 tctaaccttt ctgccagcgg ggtgtcgtat tgcttttttc tcatgtccat ttagcaatta
      661 taaccgttca gtgaacacta acaattcctc gtagtgttga cttatgttct ctttaagtga
      721 acaattgttg tggctgattg aattggaggt tgtatgacag cattggacaa ggcaataaag
      781 attgctggtg gtattcgtcc gctagggcgt gcaatagggg cttggccgtc tcaaatacac
      841 aaatgggcaa atgaatacaa tggtcgagtg ccgacagggg agcgggttcg tcaaatttat
      901 attgcaactg gggtgactcc tcatgaattg cgaccagatt tatatccgaa tccaaccgac
      961 ggtttacctg ttggatgtaa ggctaacaca caaaatacac cggagttgat tcatgaaaat
     1021 caggcatgag cacatcgaat cagtgctgtt agccctggca gccgaaaaag ggcaggcgtg
     1081 ggtcgctaac gcaattactg aagaatatct gcgccagggg ggcggcgaat tgtccctggt
     1141 accaggcaag gactggaata atcagcagaa catctatcac cgctggttga aaggtgaaac
     1201 aaaagcgcaa agggaaaaaa ttcagaaact gctccctgcg attctggcaa ttcttccgcg
     1261 cgagctgcgt caccgactct gcatcttcga taccctggaa cgccgtgcat tactggcggc
     1321 gcaggaagcg ttgagtacgg caattgatgc gcatgatgat gcagtccagg ccgtttaccg
     1381 gaaagcatat ttcagcggtg gtgggtcgcc cggcgattct gtcgtagtgc attgattgaa
     1441 attaatcgtg ccggattgtt ttgttcggta tcagttaaat gtaacgctgc gagcgttaca
     1501 aggtgaaaac aaatggcttc aaactggata aagctcgagg ttattacgcc ggataagccg
     1561 gaaatattca ggcttgctga gattctgaat attgatccag atgccgcatt agggaaggtt
     1621 attcgcttct gggcatgggc ggatcaacaa atgatagacg gtaatgcaga ttgtaacgct
     1681 cgcggcgtta caaaaagtgc aatagatcgc atcactttta tggctggttt tgctgatgcg
     1741 ttaattcagg ttggatggct ggtcgaaaat gacggtgggc tttctctacc taactttgaa
     1801 cgtcataacg gaaaaagctc taaaaaacgg gcggttacaa acgagcgagt tacaaaaata
     1861 cgcgaactga aacgaaaatg taacgctgcc agcgttacac aaacggatca aaaagcgtta
     1921 ccagaggaag aggaagagga agatctaaat actgatctcc ccctaaatcc ccctcgccaa
     1981 aaacgagcgt ctaaaaaatt cgagccggag gctattgatc tgcccgattg gttgccggaa
     2041 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
     2101 cagggcgcta acggggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct
     2161 gagcaggtga ttcgacacag catcgccaat gaataccagg gcttgttcgc accgaaaggt
     2221 gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc
     2281 ccgccaggct tcagggggta acagaccatg aaaaatattg cgacaggcgg cgttctggag
     2341 cgtatccgca gactgacccc accacatgta accgccccat tcagaacggt tgcggagtgg
     2401 cgtgagtggc aacttactga aggccagaaa cgttgcgagg agatcaaccg tctgaatcgt
     2461 cagttgcggg tggaaaaaat tctgaatcgc tctggcatcc agccgttgca ccgtaaatgc
     2521 tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa
     2581 tccatcgctg atgaactgat gaccgggtat acaaattttg cgttcagcgg aaaacctggt
     2641 accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca
     2701 gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgacggg
     2761 cagtcaggcg aaaaattttt gcgtgaactg tgcgaagtgg acctcctggt tcttgacgaa
     2821 attggcatcc agcgtgagac gaaaaacgag caggtggtac tgcaccagat tgttgaccgc
     2881 cggacggcat cactgcgcag tgtcgggatg ctgacaaacc tgaatcatgc cgcaatgagc
     2941 acgcttcttg gtgagaggat tatggatcgc atgaccatga acggcgg
//
LOCUS       DAZFYC010000266         2904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFYC010000266 DAZFYC010000000
VERSION     DAZFYC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.272"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..821
                     /locus_tag="ACHKW5_005294"
     CDS             <1..821
                     /locus_tag="ACHKW5_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514449.1"
                     /translation="GAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHC
                     DNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERELSLAELSWWAACNQVVDAL
                     PEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQRTKNIALPLHVHQQQNPPQK
                     KTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQG
                     GVGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIELIFRFLDHAFATGVLG"
     gene            834..1208
                     /locus_tag="ACHKW5_005295"
     CDS             834..1208
                     /locus_tag="ACHKW5_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7514450.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVVLIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRAQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            1205..2026
                     /locus_tag="ACHKW5_005296"
     CDS             1205..2026
                     /locus_tag="ACHKW5_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7514451.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRKRATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            2253..2450
                     /locus_tag="ACHKW5_005297"
     CDS             2253..2450
                     /locus_tag="ACHKW5_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7514452.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            2587..>2904
                     /locus_tag="ACHKW5_005298"
     CDS             2587..>2904
                     /locus_tag="ACHKW5_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7514453.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDM"
BASE COUNT          760 a          629 c          794 g          721 t
ORIGIN      
        1 gtggtgcagg tgcactgtct gactggttat tacgtcacgt gaaatcctgc cagtggccac
       61 acggcgatta tcatcacagc gaaacagtca ttcaccgtta tggtaccggc gcgatggtgt
      121 tgtgctggca ctgtgacaac cagctgcgcg accagacatc agaatcactc gatcaacttg
      181 ctcagcagaa tctggttgcc tggatgattg atgtcatccg tcacgcaata agcggtacgc
      241 aggagaggga gttatcgctg gccgaattat cctggtgggc ggcctgcaat caggtggtgg
      301 atgcactacc tgaggcagta gcgcgtcgtt cgctgggatt accagcggaa aaaatccgct
      361 ccgtataccg tgagagtgac atcgtaccgg gagaacagac agccatcagc atactgaagc
      421 agcgcacaaa aaatattgcg ctgccacttc acgtccacca gcaacaaaat ccaccacaga
      481 aaaaaacggt tgtcagtatc gccgttgatc cggagtctcc tgaatcgttc atgaggcggc
      541 ctaaacgtcg ccgttgggtt aatgagaaat acacgcgctg ggtaaagaca cagccgtgtg
      601 cgtgttgtgg taagccagct gacgatccgc atcacctgat tggtcatggt caggggggag
      661 tgggaacaaa ggcccacgat attttcacgc taccgttgtg ccgggagcac cacaacgaac
      721 ttcatgcaga cccgctgaag tttgagaaaa agtacggctc tcagattgag ttaatttttc
      781 gttttcttga tcacgccttt gcaaccggcg tgctcgggta aaagaggtga ctgatgctca
      841 tagatttggt tttaccttac ccgccgacgg tgaacactta ctggcgacgc cgtggcagca
      901 catattttgt atcaaaagcc ggggagcgtt atcgccgggc agtggtgctt attgttcgcc
      961 agcagcgact gaaattaagc ctgtccggac ggctggcaat aaaaattatt gcagagccac
     1021 cggataagcg ccgccgtgac ctggacaata ttctgaaagc accgctggat gcgctgacgc
     1081 acgcggggtt gttaatggac gatgagcagt ttgatgaaat caatatcgtt cgtgctcagc
     1141 cagtatctgg tggacgtctg ggggtgaaga tttaccccat aatgcttgaa gggcaggtca
     1201 aaaaatgaaa ctggaagatt taccgaaata ctactcccca aaatcccccg gcctgactga
     1261 tgcatcggcc tcaacgtcaa aagatgcgct gagtatcact gatgtgatgg ccgcgcaggg
     1321 catgacacag aatcgggctg agatggggtt ttctgcgttc cttgggaaaa tgggcattag
     1381 tatgaatgac agaaagcggg caacagaatt gctgacagaa tatgcactca gtcggtgtga
     1441 tcgcgtggcg gcgttaagaa aactcccggc agaaataaaa ccggcagtga tgcgtattat
     1501 ggcttcgtat gcgtttgaag attatgcccg tagcgcggcg agcaaaaaac agtgcccctg
     1561 ctgtcacgga aaaaaattta ttgaaagcga ggtttttaca aacaagatcc agtatccgga
     1621 tggtaagccg ccagtgtggg caaagtgcac aaaaggcgtg tatccgtctt actgggagga
     1681 atggaaaaaa gtcagggagg tggtaaaagt tgcctgtccg gagtgtggag ggaagggaga
     1741 ggtatccact gcctgtaaag attgtcgtgg gcgcggtgtt gccattcatc gtgaagagtc
     1801 ggaaaaacag ggtgtgccgg ttttcagaaa ctgtcagcgt tgtggtgggc gtggctatga
     1861 aagattacct tcaacggagg catttaatgc catatgtaat gtaaccgatg ccatatctct
     1921 tgatacatgg aaaaaaacag ttaaacgttt ttacgatacg ctggtggtgc agtttgatat
     1981 tgaagaagca tgggcagaac aacaactgaa aaaggtgacc agatagcttt gttgattttt
     2041 cccgaatctg tggtaaattt gccctaacga tgggcgtttt atgcctgacg ttagaagatt
     2101 ttttacaccc cgccgcctgg cgggtttttt atgactgaaa tcgcgtcagt acagtaaacg
     2161 cgctggtggc ggtgaatacc ggtctttcag cttgctggct tttttgacaa gagttattgg
     2221 tgtgtcacgt taaccgaaaa agggaaaaag acatgctgaa acagcaggat atgacagaaa
     2281 ctgccagagc agtgtttaat gaattaagcg tcaccgaacc ggcgacagtc ggggagattg
     2341 cgcagaatac ttacctttca cgcgaacgct gccagttaat actgacccag cttgttatgg
     2401 cgggtctggc agactatcag ttcggttgtt acagacgcct tcagtcctga aggctttttt
     2461 atttgtggta aatgggcgac tggtgggggg ggcgacacct gtcagtcctt tgcttatgtg
     2521 ttgatgataa tttacctttt ggggctataa ttgagctaac caattgctaa tgaaagtaaa
     2581 attataatgg ctgttgtctg ttcagttatc atggtttgct ccccaattaa tatttttctt
     2641 gaaaaggata cgttgtcact taagccaggc tcagttgttc tggccaccaa atgcatcagg
     2701 gagcttttcc ttatgcatta tggcaaagtt aaaattgtcg atataagcga atccgtcgta
     2761 agtcaatatc tggaaagtca gcataagctg acgaggactc gtctgactga cattccgctt
     2821 tacctgttgc tggaacccaa caatcctgcg ttggctgcgg ctttaattac cagccaggga
     2881 ttttccggag aggccacgga tatg
//
LOCUS       DAZFYC010000267         2885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFYC010000267 DAZFYC010000000
VERSION     DAZFYC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.273"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHKW5_005299"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHKW5_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO7514454.1"
                     /translation="MKHREIRAAVLSALKDNISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHKW5_005300"
     CDS             complement(399..1022)
                     /locus_tag="ACHKW5_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514455.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHKW5_005301"
     CDS             complement(1025..1306)
                     /locus_tag="ACHKW5_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO7514456.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHKW5_005302"
     CDS             complement(1299..1625)
                     /locus_tag="ACHKW5_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO7514457.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..>2885)
                     /locus_tag="ACHKW5_005303"
     CDS             complement(1713..>2885)
                     /locus_tag="ACHKW5_005303"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO7514458.1"
                     /translation="AQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNF
                     VGDGIRQAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALT
                     HSTSDFGNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQV
                     REGAEYKYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIG
                     DLVYKVLTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRP
                     AFMLVPVALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLA
                     AAQGTDTIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
BASE COUNT          608 a          917 c          700 g          660 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatattgt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcgccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcgtaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtccg cgccggtgga tgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcgtcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccgcccc ctttcagggc cgcacgctgt
      721 cctttctttc tccgttttct gcgggaaagt cgaacgcggg cctcccccag tttgatggcg
      781 ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct tatccggtcg tgccggactt
      841 aaccggatgc gggcacggat aagacgacgg ggaacggcca gctccctggc aactgaagag
      901 gccgttttcg caataatggc ccccgccact cggttcagtg tcgtggcaga ggcccgggga
      961 acggcacggc gatcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc acccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg ggcattttcc caggctaccc ccgctgccac cgcaccgtct
     1381 gcagccagct gcacaacccc gtccttcaga taaaccaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggccgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtcccc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagtcgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgc
//
LOCUS       DAZFYC010000268         2853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFYC010000268 DAZFYC010000000
VERSION     DAZFYC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.274"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..469
                     /locus_tag="ACHKW5_005304"
     CDS             <1..469
                     /locus_tag="ACHKW5_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7514459.1"
                     /translation="FNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIYAYAFDYHE
                     KGNVTDAEIYYKFLCIYAFENHEYLEGFASVCQSKKKYQQAYDLYKLSYNYFPYDDYS
                     VIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
     gene            803..2179
                     /gene="ygeH"
                     /locus_tag="ACHKW5_005305"
     CDS             803..2179
                     /gene="ygeH"
                     /locus_tag="ACHKW5_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO7514460.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTLESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            2347..2565
                     /gene="ygeI"
                     /locus_tag="ACHKW5_005306"
     CDS             2347..2565
                     /gene="ygeI"
                     /locus_tag="ACHKW5_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO7514461.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            2648..>2853
                     /locus_tag="ACHKW5_005307"
     CDS             2648..>2853
                     /locus_tag="ACHKW5_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7514462.1"
                     /translation="MRSPKVKFLTIFTFCIFITKMSFASNSCSNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNI"
BASE COUNT         1049 a          476 c          466 g          862 t
ORIGIN      
        1 attcaataat agtgatgaat gggcaaatca actaaaacac ctattatcga aaggagaaaa
       61 tctggcatta ctacatggtt taactcctga tatccttgat agaatatatg catatgcatt
      121 cgactaccat gaaaagggta atgtaacgga cgcagaaatt tattataaat ttttgtgcat
      181 ttatgcgttc gaaaatcatg agtatctaga aggttttgca tcagtatgtc agtcaaaaaa
      241 gaaatatcaa caagcatatg acctttacaa actaagttac aattacttcc cgtatgatga
      301 ctattcagtt atttatcgta tgggtcaatg tcagattggg gctaaaaata tcgataacgc
      361 aatgcaatgt ttctatcaca ttattaacaa ttgtgaggat gatagtgtta agagtaaagc
      421 gcaggcatat attgaactct taaacgataa ttcagaagat aatggctaag cattacaatc
      481 atttggttaa atttgctggt tatagtgcag ttaaccacta taaccagcac ctcttatgtt
      541 ttcttaaatc agcatcttaa aacagttaag agcatactat aaatcattat ttttcaatgc
      601 gttagagaac aatctacttt atcaaaattg aataaggcta tttcagatta catcactgaa
      661 ttcctaagat aaattagtga acacaaccat agaatgtcga atgtaatttc ctgtagcgtt
      721 aatgacacat aaatatgaat agccataatt tctattgctt aagcaggatg caagaaacca
      781 attttttcat agaggttaac taatggactt agaaaataaa ttctcatatc attttcttga
      841 gggattaacg ctcacggaag atggaattct tactcaagga aatgagcaag tttatattcc
      901 acagaaagag ttaggtgtat taatagtatt acttgaatcc gctggtcatg ttgtactgaa
      961 agatatgatc atcgaatcag tatggaaaaa tattattgtt agtgacgagt ccctgacaag
     1021 atgtatctat tctttgcgct gcatttttga aaaaattggc tatgatcgtt gcatagaaac
     1081 aatctaccgg aaaggttatc gtttcagcgg gcaggttttc aaaactaaaa ttaatgaaga
     1141 taatacttca gactattcca tagctatatt ccctttcact acttcgttga atacactgga
     1201 tccattaata cttaatcagg aattagtgca aatcatttca aataaaaaaa tcgatggtct
     1261 ctatacctat ccgatggctg cgacaaattt ttgtaatgat cacatatctc aaaattcatt
     1321 cttgagcaga ttcaagccag attatttcgt tacaggaaga ataaaccaga ataatgcagt
     1381 gaacacttta tacattgagt tgatcgacgc taaaaacctt ttcctcatcg ccagtaatca
     1441 tctccctgtt gatgaactac ataatacatc acaatttatt atagataata tccttcaaac
     1501 ggtacataaa ccagaacgat ctgtaagatt agctaagcag gaccaaggat ataagaatca
     1561 ttatttatca gatgaaatgt tagccggaaa gaaagaactt tacgacttca cccttgaaag
     1621 catttacagg gccatgacta tatttgatag attacaaaat aaaagtgata tacagacgct
     1681 aaaaacagaa tgttattgcc ttctagcgga atgccatatg tctttggcac ttcatggaaa
     1741 aagtgaactt gaacttgctg ctcaaaaagc attagagctt ttagattatg tatcagacat
     1801 aaccactgtc gatggaaaaa ttttagctat tatgggactg ataactggtc tgtctggaca
     1861 agcaaaagta tctcatatct tatttgaaca ggctaagata cactcaactg atatagcctc
     1921 tctctactac tatagggcac tagtccactt tcataatgaa aaaattgaag aggcaaggat
     1981 ttgtatagac aaatcactac aactcgaacc cagaagacga aaagcagttg tgataaaaga
     2041 atgtgtagat atgtatgtgc ctaacccgct caaaaacaac attaaactct actataaaga
     2101 aactgagagt gaaagccatc gagttataat tgacaacatt ttgaaattaa agcagctgac
     2161 gagaatttgt atgcgataaa tttttattga cgcaatttta ttatttagat gtatgcaact
     2221 tgttatttaa acaacagaga taaaaatatc taagatattc accttattgc aagatattta
     2281 aaatgctcta gagttaaaag tatgatgtaa agaccattgg cctggacaat taaaaaaggg
     2341 agctttatga ctaatccaat cggtatcaat aatttatctc aaagttcaaa tatagcgaat
     2401 gcaacgggtg atgaagtcgt atcattagat aaacacatca acaccagtgc tactgataca
     2461 gatcaaatac aagcatttat agtttcaaca tggatggcgc cttttcaaaa tgatatgtat
     2521 tcagaagata atcctatctc accttactat aaaattgaat ggtagacgat tgaccaaata
     2581 aatcataacg agatactttt ataatagtat ctcgtccctt tcattcaatc ctcacacatg
     2641 aaaaaatatg cgctcaccaa aagttaaatt cttaaccatc ttcacgtttt gtatatttat
     2701 tacaaaaatg tctttcgcca gcaactcttg ctctaatgaa gcaggaacaa tgttcagaat
     2761 cgaaccaaat ttaattaaag ctatcgctct tgttgaaagc aatctaaaaa aggatagcat
     2821 aggcaagaac agagataaga ataacaatat aaa
//
LOCUS       DAZFYC010000269         2849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFYC010000269 DAZFYC010000000
VERSION     DAZFYC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.275"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..310
                     /locus_tag="ACHKW5_005308"
     CDS             <1..310
                     /locus_tag="ACHKW5_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO7514463.1"
                     /translation="AAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSYRKDK
                     RVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS
                     "
     gene            338..1296
                     /locus_tag="ACHKW5_005309"
                     /pseudo
     CDS             338..1296
                     /locus_tag="ACHKW5_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1308..1769
                     /gene="yibG"
                     /locus_tag="ACHKW5_005310"
     CDS             1308..1769
                     /gene="yibG"
                     /locus_tag="ACHKW5_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO7514464.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2404..>2849
                     /locus_tag="ACHKW5_005311"
     CDS             2404..>2849
                     /locus_tag="ACHKW5_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO7514465.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDKN"
BASE COUNT          922 a          495 c          639 g          793 t
ORIGIN      
        1 ggccgcattt gcaataaata tcaataaata tgataatagt gatattaggg attgttttgt
       61 ggagatgctt caggataaaa atgaggaagt ccgtattgaa gcaataatcg gactttccta
      121 cagaaaagat aaaagggttt tatctgtttt atgcgatgag ttaaaaaaaa atactgttta
      181 tgatgatatc attgaagctg cgggtgaatt aggtgataaa acgctacttc ctgttttaga
      241 tactatgttg tacaagtttg atgacaatga aattataact tccgctattg ataagctgaa
      301 gcgttcatag cgtgagttgc ctatgcacag tgggggattc ccgccggcac ggtgcagatg
      361 ctcgaccggc tggaaagtga aatcctggct gaccgggtga gtgaggaaag ccgccgctgg
      421 ctggcatcgt gcggcctgac cgtggagcag atgaaaaacc agatggaccc ggtgtacacg
      481 ccggcgcgaa aaatccacct gtaccactgc gaccatcgcg gcctgccgct ggcgctcatc
      541 agcacggaag gggcaacagc gtggtgcgca gaatatgatg aatggggcaa cctgctgagt
      601 gatgagaacc cgcatcatct gcagcagctc attcgcctgc cgggtcagca gtatgatgag
      661 gagtccggcc tgtattacaa ccgccaccgc tattatgacc cgctgcaggg gcggtatatc
      721 actcaggatc cgattgggct gaagggggga tggaattttt atcagtatcc gttgaatccg
      781 gtcataaatg tagatccgca aggtttggtt gatataaatt tataccccga aagtgatctt
      841 atccattctg tagctgatga gattaatatc ccaggcgttt tcacaatcgg ggggcatggt
      901 acccccacat ctattgaatc cgcaacgcgc agtatcatga cagctaaaga tctagcatat
      961 ctaattaaat ttgatgggaa ttataaagat gggatgacag tttggttatt ttcttgtaat
     1021 acaggtaaag gacaaaattc atttgctagc caattagcta aagagttaca tacaaatgta
     1081 ataggacctg acacgctatg gacgtggtgg gggcgaggaa ctaatggtaa gttaaaaatg
     1141 gatacagtgc taacagcacc aacgaacctt aattcaaata aggatctaat ggctataaca
     1201 acaaaagacc ttggtaattg gataacatat gggccatctg ggcaccccat ttctaatatg
     1261 caaggtacgc cagaaaaacc cagtgatata agataggttg tagatgtatg aaagcatgct
     1321 tgttactatt tttttatttt tctcttattg gtcaattgca tggtgctgat gtgaaaataa
     1381 aagaaaacga aagtgtgatg ggatctacag caatgaccta tgatttaagt gaagaaaagc
     1441 tgatgaaact gaagtataaa tcacaacatg gtgattcaga ggcatcattt cgactatatc
     1501 aatattactg ctttactaaa aataatattg ataaacaatt gcgattcttg gaaagatcag
     1561 catctcaggg gaatgttaca gcgcagttta attatggggt ctttttatca gatacaaatc
     1621 caacattatc agaatattat aatttgaata gagctattta ttggatggaa ttcgctgtaa
     1681 ataacggtaa tattgatgca aagagcaaac ttcaagagct caaaaagcta aagcgaatgg
     1741 atagaaggaa gaataaggag aatccgtaaa agcaatagtt catcagactg ccgcagcagt
     1801 agtatgatga tgaatcaggg ctgtactata actattatcg gtaacattaa ccgcagcagg
     1861 ggcggtataa cactcaggct ccgattgggc tggaaggtag gtggaatttt tatcagtatc
     1921 cgttgaaccc gatttctgga atcgatcccc ttgggattag ccacctgttt atacagtata
     1981 actttaggta tgctttcgtg tgtttcagat ataccatgtg atgataattt ttatgatgtg
     2041 cttagtattc cagtcgcttc tggtaataat atgcaatgta aaaataatcc ccgttgtaca
     2101 catttataaa tagatggcca atacctcaag gcctatggag ttagaatgtt aatgacccgg
     2161 gagcaagcaa tagaaaacct gacggcatac gattagtacc gtcagttaat acaggaactt
     2221 ataatcgcaa tggattttca atacatagtt gtttaaacgc ttttggtccg tctttaggac
     2281 ctcgattctg ttccgagggt tgtataactg gtttatctaa tgacatgcaa aaattaaatg
     2341 agttaatatt ttcagagcca gatagcacat taacagttac ggactaaaaa atatggcaag
     2401 gttattaaca attgtatttt tttctctatt tctgcgagtg cagttactgc aagacaattt
     2461 cagagagagt tagatttggc agacaaagca atattatggg cactaatatc agccagtact
     2521 aaagagggac gaaaagcgtg ttcactaagc tattttgcct gtaaggccgc agaggctgaa
     2581 ctcggactgg catatatggc tgcgaatgat aacaaggaat ttctcacatc cttatctaat
     2641 ataatgaggt acaaaataga tgctggactt tccgaatcct atacatgcta tttattaagt
     2701 aagggaaaga tcataagacc atatctgaaa aatctaaatc cactccaact tgccgctgat
     2761 tgcattgaaa cagtaaataa aataaaagat aagaataaaa aaatcattga tataaattca
     2821 gttaatattt gtagtgatga taaaaatat
//
LOCUS       DAZFYC010000270         2828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFYC010000270 DAZFYC010000000
VERSION     DAZFYC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.276"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            130..2340
                     /gene="katP"
                     /locus_tag="ACHKW5_005312"
     CDS             130..2340
                     /gene="katP"
                     /locus_tag="ACHKW5_005312"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO7514466.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2384..2773
                     /locus_tag="ACHKW5_005313"
     CDS             2384..2773
                     /locus_tag="ACHKW5_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO7514467.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
BASE COUNT          837 a          539 c          712 g          740 t
ORIGIN      
        1 ttgcggcttg gaacccctta gtattttttg tctgtagtat ctatcccagc aataggtata
       61 tcctgttgca tcaataaagt tgacttttgt atacaacatg cgaatttccc ttaatccgga
      121 gctattcgta tgataaaaaa aactcttcct gttctgattc ttctggcgct atcggggagc
      181 ttttctaccg ctgtagccgc tgataaaaaa gagactcaaa atttctacta tccagaaaca
      241 ctggatttaa ctcctctgag attacacagc cctgaatcaa atccctgggg ggctgatttt
      301 gattatgcca ccagatttca acagctggat atggaggctc tgaaaaaaga tatcaaagat
      361 ttgctgacaa cttcccagga ttggtggcct gcggattatg gtcattatgg tcctttcttt
      421 attcgtatgg cttggcacgg tgccggaaca tacaggacat atgatggccg gggaggcgcc
      481 agtggtggtc agcaacgttt tgaaccgctg aacagctggc cggataacgt taatctggat
      541 aaagcccgtc gattgctgtg gccagtcaag aaaaaatacg gctccagtat ttcctgggga
      601 gacctgatgg tcctgactgg taatgttgcc cttgaatcca tgggatttaa aacgctggga
      661 tttgctggcg gaagagaaga tgactgggag tcggacctgg tatactgggg gcctgacaac
      721 aagcctcttg cagataaccg ggataaaaac gggaaacttc agaaacctct tgccgccacg
      781 cagatgggac ttatttatgt caatcctgaa ggccccggtg gaaaaccaga tcctctggct
      841 tccgcgaaag atatcaggga agctttttca cgtatggcca tggatgatga ggagactgtg
      901 gccctgatcg cgggagggca tacatttggt aaagcacatg gtgcagcgtc tcctgaaaaa
      961 tgtattggcg cagggcctga tggtgcacct gtggaggagc agggactggg atggaaaaat
     1021 aaatgtggta caggaaacgg caaatatacc atcaccagtg gcctggaagg agcctggtcg
     1081 acatcgccaa cccagttcac aatgcagtat ctgaagaatt tatataaata tgaatgggag
     1141 ctgcacaaga gtcctgccgg tgcttatcag tggaagccta aaaaagcggc aaatatagtt
     1201 caggacgcgc atgatccgtc tgtcctgcat ccgttgatga tgtttacgac ggatattgct
     1261 cttaaagttg atcctgaata taagaaaata accacccgtt tcctgaatga tccaaaagct
     1321 tttgagcagg cattcgcaag agcatggttt aaactgaccc accgggatat gggaccggca
     1381 gcccgatatc ttggtaatga agttcctgca gaatcattta tctggcagga tcctcttcct
     1441 gcggcggatt atacaatgat tgatggtaaa gacattaagt cgctgaaaga gcaggttatg
     1501 gatttgggta tccctgcatc tgagctgata aagacagcct gggcttcagc ttccacattt
     1561 cgtgtgactg attatcgtgg gggaaataat ggtgcccgca tcaggttaca gcccgaaatt
     1621 aactgggaag ttaatgagcc tgaaaaactg aagaaagtac tggcatccct gacctcatta
     1681 cagcgtgaat ttaacaaaaa acagtctgac ggaaagaaag tgtcgttggc tgatttaatt
     1741 gttctttcgg gtaatgctgc aatcgaagat gcggccagaa aagccggggt ggaacttgag
     1801 attcccttta ctccgggaag aactgacgcc tctcaggagc agacggatgt tgcctcattc
     1861 agtgtactgg agccgacagc agatggattc agaaattatt actcaaaaag cagaagtcat
     1921 atatcgccgg ttgaaagcct cattgataaa gccagtcagc tggatctcac cgttcctgaa
     1981 atgacggcat tactgggtgg tctgcgggta atggatatta atacaaataa ttcttcgttg
     2041 ggagtgttta ccgatacccc tggtgttctg gataacaagt tttttgttaa tctgctggat
     2101 atgtcaacac gatggagtaa agcagataaa gaagatacat acaatggatt cgatcgtaaa
     2161 acgggagcat taaaatggaa agcatcctct gttgatttaa tcttcagttc aaatcctgaa
     2221 ttacgtgcgg tggcagaagt atatgcctcg gatgatgcga gaaataagtt tattcatgat
     2281 tttgttaaat cgtggaataa agttatgaat agcgatcggt ttgatttaaa caataaatga
     2341 taaaactgat gagggagggc acctccctca tgaaggagaa attatgcaga aaagcaataa
     2401 agttataatc agctgtggta tggcattata ttttttgtgt ggggggatta cagtggcaga
     2461 agaacttgat gaaagcatgc aagttcttgc aaggaatatg agtgtacttc agtcatcaat
     2521 ggataaaaag gagcttcttg aagctcttga cagaatggat gatgctgtag atgagtcaat
     2581 gaagcgtctt cctgagagca tttcaccggc tgatgaagaa gggaagaatg actatataaa
     2641 agaacttcaa aagcttgaaa aagagattat gctggcgaaa caaaaagtct tatcgggaca
     2701 gttatcagat atacccgatt cggtaaacaa gatgaataaa atcagagttg aggggcatga
     2761 taaattcaga taaatcagtt ggttgattcc tgcgggacga atacatacgt caggaacata
     2821 actttatg
//
LOCUS       DAZFYC010000271         2819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFYC010000271 DAZFYC010000000
VERSION     DAZFYC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.277"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..693
                     /gene="comR"
                     /locus_tag="ACHKW5_005314"
     CDS             61..693
                     /gene="comR"
                     /locus_tag="ACHKW5_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO7514468.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(755..1294)
                     /gene="ycfJ"
                     /locus_tag="ACHKW5_005315"
     CDS             complement(755..1294)
                     /gene="ycfJ"
                     /locus_tag="ACHKW5_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7514469.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHKW5_005316"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHKW5_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO7514470.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADKGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          677 a          773 c          695 g          674 t
ORIGIN      
        1 aacggaggtg attattttta taataacctt tcattcacag tcaccaggta caacggcatc
       61 atggcaactg actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac
      121 agggatgccg cgcttgataa ggccatgaaa ttgttctggc aacacggtta tgaagcgact
      181 tctcttgcgg acctcgtcga agcgaccgga gccaaagcgc ccacgctata cgcggaattt
      241 accaacaaag aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg ttttgccgct
      301 aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat
      361 tttgctgcca tcgccaactg ctttaccagc aaagacaccc cggcaggctg cttcatgatc
      421 aacaactgca ccaccctctc cccagattca ggagatatcg ccaatacgtt gaaatcacgc
      481 catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc gcgcggggaa
      541 atcccgaccc actgtgacgt gacacatctg gcagaattcc ttaattgtat tattcagggg
      601 atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac taatgcagat tgccggaacg
      661 actttgcgtt tatggcccga actggtgaaa taagtcgcaa aaaaagcccc tcagcctgag
      721 cgaatgaggg gccaaattgc agagtacagc cttgttatac tttgttattc aaaatcagtt
      781 gcccattact atctagcggg atctgcgtac ccggatcgcg gtccatgcgg attttgccct
      841 gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac ttgtcataca
      901 ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga gagccctgga
      961 tctggttacc ggcatatcca ccacccagcg cccccacaac agtggcgaca tctttaccgc
     1021 gaccaccgcc aaactgatgc cctatcacgc cgccagcaac agcgccgagc accgacccgg
     1081 taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg cgacactcct
     1141 gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga gcgtattgcg
     1201 ggccacgttc aaacacgttc agactggcca ctgccgctac gcccagcgca gctgcgacac
     1261 caatcccgat acccgccaac attgatttat tcacgggaac acctccttct ttgcagttat
     1321 gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa
     1381 tggctggaaa tacttaagga ttcggaggag tctgaaggaa tgcctgatgc gacgcggctg
     1441 ccgatcgcgc tgttcctcaa gggcggataa ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccat tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttta tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaactag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccct
//
LOCUS       DAZFYC010000272         2815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFYC010000272 DAZFYC010000000
VERSION     DAZFYC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.278"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..924)
                     /locus_tag="ACHKW5_005317"
     CDS             complement(<1..924)
                     /locus_tag="ACHKW5_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO7514471.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKDTDQ
                     EDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARPEQ
                     SHQLQQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAF
                     SAWKARLREGVPPEAILDGVRRYARYLAATGK"
     gene            complement(996..1421)
                     /locus_tag="ACHKW5_005318"
     CDS             complement(996..1421)
                     /locus_tag="ACHKW5_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7514472.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(1405..1647)
                     /locus_tag="ACHKW5_005319"
     CDS             complement(1405..1647)
                     /locus_tag="ACHKW5_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7514473.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            2039..2377
                     /locus_tag="ACHKW5_005320"
     CDS             2039..2377
                     /locus_tag="ACHKW5_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7514474.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPNRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            2670..>2815
                     /locus_tag="ACHKW5_005321"
     CDS             2670..>2815
                     /locus_tag="ACHKW5_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7514475.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNS"
BASE COUNT          679 a          750 c          625 g          761 t
ORIGIN      
        1 tttcccggtc gccgccaggt atcttgcgta acgcctcaca ccatccagta tggcctctgg
       61 tggcaccccc tcgcgtaatc tggccttcca ggcactgaaa gcggatttct tcgggtttgc
      121 cccagcacgc aacgggtatt cccgccagac ctgttcgaac acatccggat aatccactcg
      181 tcccacaggc tgcccggtgt tttccgggac tacccgatcg gcttcccgct gaatggcgga
      241 atcggcttca ggctgctgca gttggtgtga ttgctccggc cttgcggtca tcacctgctg
      301 cacagcgccc gaatcggctt tcagcgcata cgctgaatcg gcttccggtg tcgtgcctgc
      361 tggctgacca ggattgacgg tctgaacatc ccctgcctgg ttcgtggcgt tttttacgcc
      421 atggaccata gtgttttgat cttcttgatc tgtatcttta tctgtatctt tatctgtatc
      481 tttatctgtc gtgactcgtc gtgacatgtg cgtgacattt cgtgacgcgc cgtgacaatc
      541 gccattttgt tcccgctttc tttccctctc tcgctgcgcc ctcttgcgct ctgcaggaga
      601 ttttgcggtt tgcgaaatat tgccgttgtc ctctttaagc acctggcgtt tttcccatcc
      661 agtgattaaa tcaccatcaa gtacccgccc ctgcatcgtc tgcaaaattg aatcaattac
      721 ctcttctgtc acgtcgagcg cacttgccaa atcttctgtc gtgacatcaa tgtgacctcg
      781 cgtgacattt cgtgacgcgc tcaccaggag gtggatatac actgccatca ctgttgcaat
      841 tggctgccct gacaccctgg caattgttcg ccacttaggg tcatttggca tgtcatgcca
      901 taatctgagc caggcgttag ccatactcac ctcttctgat accgaatctt tttactcacg
      961 agttgccgga agcgattcga tacggctatt gtcagtcaat gtactgccac agcatttcct
     1021 gccgggccac cacggttcat ctgattgaaa ccggcgattg ccactgcgac aaaatcatca
     1081 gcgtctctca ccagtcgctc ccgcgtctcc accagctccc gaaaataagc tgaactgtgg
     1141 ctgcgcattc tggccaccag caaaggtggc attgcctttt cgatcgctgg taacaacgcc
     1201 tgaatttttt caactgcatc aggggtgtct ttctctaccc agcggaaaat tttctgggta
     1261 ttgcgagcca gggcttccgg atggctgtcg tcatacagtt caggaaacgt catacccaac
     1321 tcaaaataag cctgggttat tccagctgcc ggaacttttt cgccatcagg acgcgcccag
     1381 gcattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatccccc cttggttaga
     1441 aggcggatta tgatcagaac cgggaatgac aaccgtcggt atgtgtaact catatttgag
     1501 cgccccggca gtgactgcct gaattagcaa cgcccatttc cacggaacct cttcccccca
     1561 catgctgact gtggtttttg acgttcctag agctgcggct gttttaacaa ctccgccaaa
     1621 atagcctaat acttctgatt ttttcatgag tcgctccata aaactgaacg ccaaaagttt
     1681 aataatcaaa accaaagaaa gtcaagaaac aaaaccatct gtgttttaaa atcaaaacat
     1741 gagcaagcaa acaatatctg aacgcataac ccaacgtatg catgcgctaa acctgaaagg
     1801 caaagacctt gtcaatgcca ctggcgcatc aaaaggctcc gtaagtcaat ggatgaacgg
     1861 tggaggagcg ccgtcctcgc gttacataag ttcactggca aaaatattga aagtaaacga
     1921 aaattggctt cttaatggag gagagttaaa tacaggtgat tcgcttgatc tatctttacc
     1981 gccgataaaa acggttccgc tactatcact tcagcaggca gcaagctgga gtgattatat
     2041 gaaaaattcc tcaataacct cttgtgtgca gcttgtcgga gaaatcccgg ccaatacctt
     2101 tgcagttgtt ctagagagtg acagtatgtc aacatctggt gggggagttt ccatcccaaa
     2161 tggttcaaca gtttttgttg atcccaatcg aaccgtacaa ccaggaaata ttgtccttgc
     2221 cttacccaaa gggaccacaa cacctgtcat tcgtaaactg gagatagaag ggccggatat
     2281 tcttttagtc cccacgaatc ctcgctaccc ttcaattatg ctggatgatc tatcttgcat
     2341 attgggcgta tgctttaaaa ttcaacaaga tatttaaccg acctcatcta tttgattaac
     2401 tgtatgccat cgtggtgatg gcttaacagc tgcctgctta aaatgttttg ataaaaaaac
     2461 attgacctga aaagttcatt tttctaaact tcattcattc cctctcccca ccccacagaa
     2521 tgcagggcaa tacttcgagt taccaggcag tggtcagggg ttaagtagcc agcccgaggc
     2581 gtaagaacat gacggcaggg ttcaacttta ataactatgc agcaggtttt tgttccgcta
     2641 ccccggcgtt aaggggaaat gaggtcagca tggatactat cgatcttggc aacagcgaat
     2701 ctctggtatg tggcgtgttt cccaaccagg acggtacgtt taccgcgatg acgtatacca
     2761 aaagtaaaac gtttaaaacc gaagctggcg cgcatcgctg gttagcaaga aactc
//
LOCUS       DAZFYC010000273         2809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFYC010000273 DAZFYC010000000
VERSION     DAZFYC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.279"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            210..935
                     /locus_tag="ACHKW5_005322"
     CDS             210..935
                     /locus_tag="ACHKW5_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7514476.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            893..1570
                     /gene="bglJ"
                     /locus_tag="ACHKW5_005323"
     CDS             893..1570
                     /gene="bglJ"
                     /locus_tag="ACHKW5_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO7514477.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIDARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1608..2396)
                     /gene="fhuF"
                     /locus_tag="ACHKW5_005324"
     CDS             complement(1608..2396)
                     /gene="fhuF"
                     /locus_tag="ACHKW5_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO7514478.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2537..2773
                     /locus_tag="ACHKW5_005325"
     CDS             2537..2773
                     /locus_tag="ACHKW5_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO7514479.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYILLPSCLALSAGIMLMLMNLNQG"
BASE COUNT          743 a          603 c          705 g          758 t
ORIGIN      
        1 aatgtctcca ggctatttca ccaggttatc atttgatgct aaccttctga cacatgcagt
       61 ggagttgttg tgcagcagga gtatgctgat atgaaagtga atgctaagga taatttattc
      121 gcttaatcta ttaatttgct ggaaacattt aaggaatgaa tatcctgaat aaaccatcat
      181 aatttttaga cagtggatgt ggaggaaata tgttgccagg atgctgcaaa aatggaattg
      241 ttatcagtaa aatacctgtt atgcaagcag ggttaaaaga ggtcatgagg actcacttcc
      301 ctgaatatga aataatatcc agcgcctctg cggaggacct taccttatta caattacgtc
      361 gttccggatt agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg
      421 aacattatta ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac
      481 gctcctggta ttcccaggca gtagaactgc tcatgtgccc tacggcgacg ttattgtctg
      541 atgttgaacc cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc
      601 gtatcagcgc catgctgact tctccggcaa tgactgaaac tcatgatttt agctatcgct
      661 ccgtcattct cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca
      721 tcaaccagat agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca
      781 gtgcgatgcg tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg
      841 cgcagggtgc aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca
      901 cagccgaatt aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg
      961 tattgagagt ttattcagaa agtttgcggg taacccttat aagctccata cctataccag
     1021 tcaggagtca tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt
     1081 ttctgccatg agaagtgagc gcagagaggg attatcttgc ctgactgaac tggcgattaa
     1141 gtttccgcgt acccggcgtt tagttattgc ggatgatgat attgacgctc ggctgattgg
     1201 ttcattgtcg ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt
     1261 tcatcaggaa ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa
     1321 tcagtggtac attaaccaaa gccggacgct aagccccacg gagagagaaa tattgcgctt
     1381 tatgtcgcgt ggctactcaa tgacacaaat tgccgagcag cttaaacgca atatcaaaac
     1441 gatccgtgca cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt
     1501 gttggaggcc gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca
     1561 tccctattaa tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta
     1621 caatcgccac attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc
     1681 agcaggccat cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc
     1741 gttttctcaa aaaagagggc atggcgcagc gattcaaccg tcgcctcgcc gagcagctgc
     1801 ttcatctcag tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg
     1861 ttgatctctc cggtcgcttc tagtgcctgc acaaccggaa ccagcgcctg gctgattaac
     1921 gtttccattc gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc
     1981 cagaaacagg cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca
     2041 tctaatgcct tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata
     2101 taccattgtg cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg
     2161 cgatagatat gatcggaata gaccgcgagt agagaactta gcgcattcgg tgatgaccat
     2221 tgcgccagcg tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctcgagcaaa
     2281 tgttcacgat gtttggcgat cgtcgcacgt acggcttgtg caagcccagc atcctgcggc
     2341 tggagatgcg ttcgccagat gatatcttca tagagcggtg cggaacgata ggccataatc
     2401 gggatagtaa tctaaatgat aatgattgct aatcctatcg ataggtttac ccgatagcaa
     2461 gggatttctc tggcttgcaa atgataaaaa ttatcatatg atattggtta tcattatcaa
     2521 tgaaagagat gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc
     2581 gggattgctg attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt
     2641 cattctgatg ggattgatat tgacgccggt gatgctgtat cacaaacagt tacggcatta
     2701 cattttgcta ccatcgtgcc tggcgcttag tgctggcatc atgctgatgc taatgaattt
     2761 gaatcaggga tgaaaaatca aggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DAZFYC010000274         2791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFYC010000274 DAZFYC010000000
VERSION     DAZFYC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.280"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..810
                     /locus_tag="ACHKW5_005326"
     CDS             <1..810
                     /locus_tag="ACHKW5_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO7514480.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            877..>2791
                     /gene="rhsD"
                     /locus_tag="ACHKW5_005327"
     CDS             877..>2791
                     /gene="rhsD"
                     /locus_tag="ACHKW5_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO7514481.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRS"
BASE COUNT          630 a          768 c          922 g          471 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt
      961 ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg
     1021 gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc
     1081 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg
     1141 gtgggagtgt ttggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgcgat
     1201 gatgcactgg tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg
     1261 ggggaggcgg tgtacagccg cagcgagtca atgtggctgg tgcgcggtgg taaggcagcg
     1321 cagccggacg gccacacgct ggcgcggctg tggggggcgc tgccgccgga tatccggtta
     1381 agcccgcatc tttacctggc gaccaacagc gcacaggggc cgtggtggat actggggtgg
     1441 tcagagcggg tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg
     1501 cttaccgggc tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc
     1561 gacctggccg gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta
     1621 ctgaccacac aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac
     1681 agttcccgcc ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc
     1741 cccgacaggg gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa
     1801 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
     1861 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     1921 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
     1981 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
     2041 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2101 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
     2161 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
     2221 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2281 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
     2341 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
     2401 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
     2461 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
     2521 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
     2581 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
     2641 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
     2701 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     2761 agcaccacgc agggcgggct gacgcgcagt a
//
LOCUS       DAZFYC010000275         2749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFYC010000275 DAZFYC010000000
VERSION     DAZFYC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.281"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..608
                     /locus_tag="ACHKW5_005328"
     CDS             <1..608
                     /locus_tag="ACHKW5_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO7514482.1"
                     /translation="RDHVIYINTNILDEAVWVKQALEKNQPGKPVRVINPDDESIRIF
                     SWLADNFPDLQYFKLQLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISI
                     AVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQ
                     WGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQE"
     gene            622..862
                     /locus_tag="ACHKW5_005329"
                     /pseudo
     CDS             622..862
                     /locus_tag="ACHKW5_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            882..1216
                     /gene="sctI"
                     /locus_tag="ACHKW5_005330"
                     /pseudo
     CDS             882..1216
                     /gene="sctI"
                     /locus_tag="ACHKW5_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            1213..1947
                     /locus_tag="ACHKW5_005331"
                     /pseudo
     CDS             1213..1947
                     /locus_tag="ACHKW5_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            1963..2544
                     /locus_tag="ACHKW5_005332"
     CDS             1963..2544
                     /locus_tag="ACHKW5_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO7514483.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSSYIRQQPLAMQQRFNLLFPDFVEHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
BASE COUNT          943 a          440 c          523 g          843 t
ORIGIN      
        1 gtagagatca tgttatctat attaatacca atattttgga tgaagcagtc tgggtcaaac
       61 aagcactgga aaaaaatcaa ccaggaaagc cagtacgggt gataaatcct gatgatgaat
      121 cgatacgaat tttttcatgg cttgctgata atttccctga tttacaatat tttaaacttc
      181 aactattaga tgccagtaat ctaagattga cagtgagtaa gcaacggaac gctatcacac
      241 agcaactaat cgataatctt attaaaggat tactgcaaac tatgccatat gccagcaata
      301 taagtattgc ggtattagat gataatgtat tggaaagtca ggctattgaa acgttgtcag
      361 cgataggtct ttcttatgaa aaatataaaa cagctaacaa tgtgtatttc aatatcattg
      421 gtacgttaag tgacagtgaa ttaaataaaa ttaataacta tgttgacgaa tattataaac
      481 aatggggtaa acaatatgta agatttaatg tgaatttgaa aaatcaggac acaaataata
      541 gttcatttag ctacggagat aaccgattcg agaagtctca aggtagcaac tggacatttc
      601 aggaataatt gaggaggact tatggcagat tggaatggtt atattatgga tatcagcaat
      661 caatttgatc agggcgttga tgatctgaac cagcaagttg aaaaagcgtt ggaggattta
      721 gccaaccaat ccctccgaca cgaaatttct tgccgaatat caaagtgcat tagctgagta
      781 tacattatat cgaaatgcgc aatctaacgt tgtaaaagcg tataaagatc ttgattcagc
      841 aatcatacaa aacttccgct aaactggata aggattttat tatgtcagta agcaatatgc
      901 cacctattga tagagctgag caatcaactg ctcatgaaat acagcaagcg aaagtgattg
      961 atttaaatga ccgggttctc aatcttgaca acccggatga tgataaaatg atctccgctt
     1021 ttgccaacta tgctgtacaa actgaaaatt ggcagcaaaa tgcgttacaa gcattaaggt
     1081 ctgataaaga ggggttaact cctgaaaaat tattagtctt gcaggatcat gtgcttaatt
     1141 ataatgtcga ggtttcattg gtggaacatt agcaagaaag attgtcgctg cagttgagac
     1201 gttgacccgc tcatgaaata tatatcatta ttattgttta tcctgctgtt gtgcggctgc
     1261 aagcagcagg aattattgaa ccatcttgat caacagcaag ctaatgatgt ccttgctgtt
     1321 ttacaaagac ataatattaa tgctgaaaag aaggattaag gcaaaacagg attttcaata
     1381 tttgtcgaac caacggattt tgcttctgcg gtggattggc taaaaatcta caatctaccg
     1441 ggtaagcctg atattcaaat atcgcagatg ttccctgcag atgcactcgt ttcatctcca
     1501 cgagcggaaa aagcaaggct atattctgct attgagcagc ggttagagca atctctcaaa
     1561 attatggatg gcattatttc ttcgagagta catgttagtt acgatgttga taatggcgat
     1621 agtggtaaaa ctgctttacc aattcatatt tctgtacttg cagtatatga aaaagatatc
     1681 aacccagaaa taaaaataaa tgatatcaag agatttattg ttaatagctc tgcaagcgta
     1741 caatatgaaa atatttctgt tgtattgtca aaacgaagag acattattga gcaagcacca
     1801 acatatgaaa ttagcgaacc tgtttttgct tatgataagg ctatgccggt ttctatctta
     1861 cttgcattaa tatccgtagc gacttgctgg ctgttatgga agtatagggc cattctaact
     1921 aatttagtca ggttgaaaat taagtagaca tatgagagcg ttatgaatct tgccctaaga
     1981 aagattatat atgatccaat tagttatatt catcctcaac gagtaagctt gaataataca
     2041 cctattaaca atccggtgct tagatctatt accaatgaaa tgattgttct gcagtacaat
     2101 ctttcagttg aacattttaa tctgaatagt tcacttattt actacataaa caattggaat
     2161 ttatttccat tgttctgttt attcagtggt tatcactttt atcgagaaag gtttgcagaa
     2221 cgcggatttt tttataaggt tcctgctgtg ctaagggatt atttatcagc tataccggtg
     2281 aaaataaatg aaaaggcacg atacaaaccc ggcatcgcta gctatcacaa tatcatcaca
     2341 tgtggttttt caacgttgtc atcctatata aggcaacaac cattggcaat gcagcaacga
     2401 tttaatttat tatttcctga ttttgttgaa cacatacaat tgccgttacc acttgcatca
     2461 actcttttag agagaattac attttatgcg aaaaaaaata gagatgagct tgataaaatc
     2521 tcctgcaaat ggtgttgtga ttaaaagaaa aatttctgat ggattgaaat aaatagtttc
     2581 tcttaaagaa agtatccttc ttgaaaccag cgcaaaaatt cagagcattg aggtaaagag
     2641 agaagaaaaa tttattcaag gttattatga tggctataca aaaggcatta ttgatgtgat
     2701 ggataatttt atacccttga taagcttgtt gagctcagaa ctagaaaaa
//
LOCUS       DAZFYC010000276         2697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFYC010000276 DAZFYC010000000
VERSION     DAZFYC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.282"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..1152)
                     /locus_tag="ACHKW5_005333"
     CDS             complement(10..1152)
                     /locus_tag="ACHKW5_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO7514484.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSAAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            complement(1165..1743)
                     /gene="espA"
                     /locus_tag="ACHKW5_005334"
     CDS             complement(1165..1743)
                     /gene="espA"
                     /locus_tag="ACHKW5_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO7514485.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNEVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIAEVQSSSNKDAKAK
                     LPQEVIDYIKDPRNGITVSDVKDITVELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARADIQSLQYRTISAISLGK"
     gene            complement(1801..>2697)
                     /locus_tag="ACHKW5_005335"
     CDS             complement(1801..>2697)
                     /locus_tag="ACHKW5_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO7514486.1"
                     /translation="SSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLALTDS
                     DKLKELSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLL
                     DLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDLP
                     EREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISINK
                     DKDVILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFID
                     REQRDNVLLMIGKVIDYKEDVI"
BASE COUNT          755 a          643 c          484 g          815 t
ORIGIN      
        1 ctttaataat taaactcgac cactaacaat acggctattt actcgtgctg agtcggatat
       61 taactgagaa gcattttgta aatagcttgc ctggtttcgc aactcggctg cagctttatc
      121 cagctcaacc ttcgcactct cacccaggtt ttcactctgt cgagttaagt tttgcaccgc
      181 gtaagcagat aactgagcca atatgctgta ttgcgcagaa tcattattca gcacagttgt
      241 cccaatagaa cgtgcagagg tcgtaaatcc atttgccgtt gccgcaactt ttgaaacaac
      301 gttctcaaca aatacatcgg ccagtttagt aactgttttc acgatgtttg aaccaacttt
      361 gttagcgaca tcgccaactt tagacataag tgaagacacc ccgccaatgc cggctgtcag
      421 aatacttgca gcaatagata ctccgccaaa gacttgcgct gctgtcttta acgcctgcgg
      481 ggccttatcg cccattacat caactgcagt ttgcagtgcc attgctgttg cgctaacggc
      541 aacaacagcc cagagtgccg ggttaaaaat agctgcaata gcagttaccg cgacgcccaa
      601 ccaaccaaag acctgaccaa caattttact tttttgtgct ttctcttcgg ctttctgttg
      661 ttcttcgagc tgctttttaa actccagcgt tttattatcc agcgccttat tttgcccatc
      721 cgtgtagatc tcgttggagt ttttcatact cgcaactttc tgcgctgaag catccaggga
      781 taacagggtg accatcatca tcatggcctg agggtcaacg ttattcacct gagaaagata
      841 aggatagcca gagcttgcat tctgagtgct gcgctgtggt tcggttccct ctgttcgact
      901 cctaacttca ccactaatac caccaaacag tttactcagg acatcctcag cagcagaggg
      961 cgtcactaat gagtggcctg ccggtggcgt tggtaaagcg gtactttctg tccaacttgc
     1021 tgatggggcc gtggatttaa ccagttgtaa atccagcgcg gaagtcactt caggctgatt
     1081 aatacctgaa gtagccgtag cagcgctggc cccagacctc atcgactgga tatcgctatt
     1141 tacattgagc atagttatct ccggttattt accaagggat attgctgaaa tagttctgta
     1201 ttgcagtgac tgaatatcag cacgtgcact cgttaataag tttaacgtat ttgacatttg
     1261 ctgtatttca agctggctat tattcactac cgtggtcaag ttattcgatt tggccgaaat
     1321 agctgccttc accgtttgca aatcaccagc gcctaattca acagtaatgt cttttacatc
     1381 actgaccgta atgccattac gaggatcttt tatatagtcg atcacttctt gaggaagttt
     1441 ggctttcgca tccttgttag aactgctctg aacctcagca attttagcat ccacaagatt
     1501 agccattttt tgggcagtgg ttgactcctt agatgcctca ttcatatcag caaactttgc
     1561 aatcgacaga tcgctttgtg cctgatacat ataggcaaac atgagaagcg cagcctgaaa
     1621 aacaccgact tcattaaata gctgaactac ttcgtctttc gacatgctgc ctaagtcata
     1681 gactgtcgat gtcgaagtac tcgcgttcgc actggcaact gatgttgcag ttgatgtatc
     1741 catgtatata cctcttaata atctttctta gcaagaccaa aaacaattaa aaaatcaata
     1801 tcaaataaca tcctccttat aatcaattac tttaccaatc attaataata cattatctct
     1861 ctgttcccta tcaataaaac aagcgtcagg taatgatgca aaaaagtctt tcaataggtg
     1921 gaaataataa acatgtttat cttcgacaat cgatacccga gaagggcttt ccaggagcca
     1981 gtcttctgtt aaccatacat gttcaataat agacaatact tcatttaaaa tgacatcttt
     2041 atctttatta atagagattg caataatatc tgtatagtta tcaagcgata aaaaaatgat
     2101 tacccggcat aaatcgctaa taattgaaga tgttttcacc ttatcttcaa aagaagacat
     2161 ataagacaga tcgaacgata gcgccctaat aataaccttt aagtgcttct cacgctctgg
     2221 aagatcctta atctccataa accagtcaag taaagaggct cccttttcag aatcctcatc
     2281 cttggccttt tcataaagct ttttgatctt ctggatacct tcctgattta actcacccac
     2341 ccctaaccag gaaagcagag ccaactccca atcatcttcg cctaataaat ctaataataa
     2401 tttggcgtat ttctttttta attcaatctt aacaaactta gattgtaata aagaagatat
     2461 aagtgcaaca atctcccctt ttggcatatt accaaacata gccaaattag gatcatgctg
     2521 ttccagcagc ccactgttct ttaatgatag ttcttttaat ttatcgctat cggtcagagc
     2581 aagcagctct tgcatatcat taagcagctt ctccgttgtc gttccttcct cttctttcct
     2641 tgcactgcct ttgcgatatc ccaggcttag cccttcaatc gtttcagaaa gcgatga
//
LOCUS       DAZFYC010000277         2683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFYC010000277 DAZFYC010000000
VERSION     DAZFYC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.283"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            694..1662
                     /locus_tag="ACHKW5_005336"
     CDS             694..1662
                     /locus_tag="ACHKW5_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFO7514487.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVP
                     LRCPDYTSVSKRAKSVNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSNTHEIICANLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTQLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRLTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            complement(1802..2188)
                     /gene="stbA"
                     /locus_tag="ACHKW5_005337"
     CDS             complement(1802..2188)
                     /gene="stbA"
                     /locus_tag="ACHKW5_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="HFO7514488.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEY
                     SFSVTAKGLEMALYRTRQNRKNVLHNTHDKSSAKGAAESVLHNTQPSEPEVQESKKAE
                     SPGIIDKEFFKKIGENFDPKKFNKKF"
BASE COUNT          662 a          676 c          669 g          676 t
ORIGIN      
        1 cggcaaggtg ggcggattcc cacacggcac cggcgcgcgg caaggtgggc ggatttccac
       61 acagcaccgg cgcccggcaa ggtgggcgga ttcccacacg gcaccggcgc gcggcaaggt
      121 gggcggattt ccacacggca ccggcgcccg gcaaggtggg ccgattccca tatcgacatg
      181 tatgtagctt gtgttatctg tggattgtgc agctcagcgg gtcgctggtc gtatggcgga
      241 gtgtcccccg taaccggccg cgtgcggccg ctaacgcgca gtacggcgcc gcgtcccgca
      301 gacgggccgc cgttcccgcg cgcagcactg cgcacccccg tgggggacgt acggcagctg
      361 cgtggcggtg agcgggatta gggctttgca gctaccgtgt taagttccct ggtgtcgctg
      421 aaatagttga tgaacatccc cctaagcgtc agcagcaagc tgccgccgct taggccccgg
      481 aaatcacgcc ctttacgtcg taaacgccgt aaagcccgct caccggggtt aaaagggatt
      541 actttgactt cttcattgct gttcgcgagt taaggctaat cagggcaggg ggtagggtgg
      601 cagaatttat agcccggcaa ccggctacgc cgggctttcg gcgccatttt acaacacaga
      661 cggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc
      721 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa taaccaactg gcccacctac
      781 aataaagccc tcatcaaccg tggctccata actttctggc tggatgatga agctattcag
      841 gcctggtatg agtcggcaac gccttcatca cggggaagac ctcagcgcta ttctgatctc
      901 gccatcacca ccgttctggt cattaaacgc gtgttcaggt tgaccctgcg ggctgcacag
      961 ggttttattg attccatttt tacactgatg aatgttccgt tgcgctgccc ggattacacc
     1021 agtgtcagca agcgcgcaaa gtcggttaat gtcagtttca aaacgttcac ccggggtgaa
     1081 atcgcgcatc tggtgattga ttccaccggg ctgaaggtct ttggtgaagg cgaatggaaa
     1141 gtcaaaaagc atggccagga acgccgtcgt atatggcgaa agttgcatct ggcagttgac
     1201 agcaacacac atgaaatcat ctgtgcaaac ctgtcgctga acaatgtgac ggactcagaa
     1261 gccttcccgg gtcttatccg gcagactcac agaaaaatca gggcagcatc ggcagacggc
     1321 gcttacgaca cccaactctg tcacgatgaa ctgcggcgta agaaaatcag cgcgcttatc
     1381 ccgccccgaa aaggcgcggg ttactggccc ggtgaatatg cagaccgtaa ccgtgctgtt
     1441 gcgaatcagc ggctgaccgg gagtaatgcg cggtggaaat ggacaacaga ttacaaccgt
     1501 cgctcgatag cggaaacggc gatgtaccgg gtaaaacagc tgttcggagg ttcactgaca
     1561 ctgcgtgact acgatggtca ggttgcagag gctatggccc tggtacgagc gctgaacaaa
     1621 atgacgaaag caggtatgcc tgaaagcgtg cgtattgcct gaaaacacaa cccgctacgg
     1681 gggagactta cccgaaatct gatttattca acaaagccgc gcggggctaa caggtaagat
     1741 gaaattaagg ttttacgaac actgccactg taattaatta ccgctacttt cataaatcac
     1801 ctcagaattt tttgttgaac ttcttagggt cgaaattctc accgattttc ttgaagaatt
     1861 ctttatcaat aatgccggga ctctctgctt ttttactttc ctgcacttca ggctcagacg
     1921 gttgtgtatt gtgcaataca ctttccgctg cacccttcgc gctactctta tcatgcgtat
     1981 tgtgcaatac attttttcgg ttttgccgcg ttctatacag tgccatctca agccctttgg
     2041 ctgttaccga aaagctgtat tcgctgtcga ggatttcagc aatttcatgc cgcgttagcc
     2101 ctctgtccag ccagttctcg atttcattta ggcgcaatct gacagcagcc gataagttgc
     2161 ggggtttcag atctgctggt ttattcataa ccccctccgt tgtatgaaaa tgactctaca
     2221 acactcgtaa gaaggggtca atcgagtttt gtagtttatc tgtagtttta ttgtagtcta
     2281 tttgtagtct tacaacacta aggcaaaacg ttttttgtag tttatctgta gtttatctgt
     2341 agtttttggt tgctcagatg gtcatgcaag catgaccatc aaagaatagg acgcttttta
     2401 gccaaatgcc ccttaaccta cggagatcgc cctaccaaaa aaagatgatt ttttatccct
     2461 taacctgcta tacacctaac gcagcgtgat aaatcacgct atcgcactga tttaaaagaa
     2521 aaaaaacaaa atttagccat gctgcggtat tacccaggta ttacaaatca aatctaaatt
     2581 actgatttaa ataacttttt atttattact ttggtaatac tatgatttca ccgtattaga
     2641 cacccttttt tcgtttaaaa aatgcacaaa ttgaggactg aat
//
LOCUS       DAZFYC010000278         2609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFYC010000278 DAZFYC010000000
VERSION     DAZFYC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.284"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(287..861)
                     /locus_tag="ACHKW5_005338"
                     /pseudo
     CDS             complement(287..861)
                     /locus_tag="ACHKW5_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            1018..1272
                     /locus_tag="ACHKW5_005339"
                     /pseudo
     CDS             1018..1272
                     /locus_tag="ACHKW5_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1413..2279)
                     /gene="cif"
                     /locus_tag="ACHKW5_005340"
                     /pseudo
     CDS             complement(1413..2279)
                     /gene="cif"
                     /locus_tag="ACHKW5_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428038.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
     gene            complement(2496..>2609)
                     /locus_tag="ACHKW5_005341"
     CDS             complement(2496..>2609)
                     /locus_tag="ACHKW5_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFO7514489.1"
                     /translation="IDISSYNISERSWSENQIMQSYHGGKQDLISVVLSKI"
BASE COUNT          799 a          587 c          431 g          792 t
ORIGIN      
        1 taataaggat taatatattt tttctaaaat accccacaaa ataaacacac cataaaaatt
       61 ctaaatacac acacctaatc tcaaaaaaaa acaaacttac tatttgactt ggattctgtt
      121 tttaacccaa tattttccga cagcatctcc acagaaaaat gcatttacgt aaagacgccc
      181 tcaagtcatc tgaaacctgt caaccacagg catactcttg tattcttgat gcctgtggcg
      241 tttttacaaa ttactgacag ctaatattcg ctcagagaaa aatattttaa ctatctttta
      301 taacgaagct acccttacca tggtcataaa aacatttatc agcacttaca accattgatg
      361 ccgttagttt ctctcgtgtc agtgggtgag gtgatttttc actaactaac cgagaaaatg
      421 cagtaacatc aaataaacta catacaactg aatcccctga attcttaata aacacccctt
      481 cctctggtat ctccaatgta atgggacatt gagcagactc tggcgggcac tgaagtctct
      541 ctggacaaac agaaaactta tattgagata ttttgctctg aataccctct tgcgattgtg
      601 cttcacctga gtccgaaagc atacttagca ttctctcact aagggctcga ggaccattat
      661 taaatcccaa aatcaataac tagaaagcaa tccgccatta ccgccactgg atgaaaaact
      721 cccatcagta gcatcataca caatactgac agtttcacca cctactgtaa tatctccgcg
      781 cccattggct gcaattcggg cacgaaaaac ctccagccca agattaaatg aatgtgatcc
      841 aatatctgag gttaatggca taacaaaact ccctatttta atttgaactc cagacttaaa
      901 tagctgtaac aaacatctgc cttatatggc aacacaaaaa ccggagccgg actccgtttt
      961 ttgtgtgtcg ggctgttcat tctatccttt caacattccg tctgattggc atttgtaggt
     1021 ccagcaacag tcagtcacga tgttagttat cttggcagtg ttctggatgc agccaaacct
     1081 atatacggaa ttaattacac atcaaacccg gcgaaaagtg ctcgtccata tctacttaaa
     1141 cttgctttga ttggtaaatc aaaccgtcgt aatcgtagac cagcagttga tgaacttgac
     1201 atgctcattg aagcccttca acaacgatct actcataaat gttcaaaaat tccgttcgtt
     1261 gatatcctca aaagttcagc ctaggctatg ctgaaccggt tttcctgaat cattttaccg
     1321 tatggttaaa ttttaacgtc ataatactta ttattatgtt tacaaataat tgcaatttta
     1381 acaaaagcta actacatagt gattttatta tctcaatgtt ttttttcaat attatcggtg
     1441 tcatactcaa acaactggaa actaaaattg tcatgctttt gatattgaat attttccttt
     1501 cgtaacattt taacattccc atcaatatca aataacgttg caatctgctc caaatctgtc
     1561 ttattttgag acatgcagga gaagtaatta caaatatcat ccagcaaaat cggagtcaat
     1621 gctttatgcg tcatccagca aaatcggagt caatgcttta tgcgtcatcc agtcctcaaa
     1681 tcttaacttt ctggtgacgc catctcctaa atctgactga tacaagaatg caggacgaca
     1741 agagttttct cctttgggaa tatcgacaat gtaagcatga ccaagcgcac catcattaac
     1801 acgtaataca aagctcttac attcaggcat acaggacagg cgttcctgaa gctgctcgag
     1861 cgatagtgtc tgttcatttt caaagcagta actgaagcca tccttgtcca gaaatagttt
     1921 cataatagca ttagcggtaa ctccgcagga gggttccaca atgcctttct ctcgccctat
     1981 aataacctca acctcctcaa tcgacatccc ccagtctgtt gccatctgtt tgatgttaga
     2041 gtcgatattt tgcattattg cagcaggatt tttctgatgt ataggtactt cgtggtcatc
     2101 actatttagc aatacattag ctttgaggtt ctgtgagcac agggaagcaa aatctcttac
     2161 atgtaaggct gaagtgtgtt gcatgggaga taatacagct tcagtattta cagatatggc
     2221 ccctgtcaga caggacgcgg acgtcggggg ggggaagggt aatgtctttc atatctcatt
     2281 cattcactct atgtttatct ataaatacag gcgaggtgca aaaggaactt ctggcatggg
     2341 cattatgtca tgtctgaaat ttttctggtg cctgtatgcc attcgccagt ctctcagcgg
     2401 cattgtccat ttctgtgacg cggcctggat tgccagccac accacttttt accaactcca
     2461 tccatgcaac aaatccacta cactggataa aattactaaa ttttacttaa taccacacta
     2521 ataagatctt gctttcctcc atgataagat tgcattattt gattttcact ccatgaacgt
     2581 tcagaaatat tataagatga tatatctat
//
LOCUS       DAZFYC010000279         2601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFYC010000279 DAZFYC010000000
VERSION     DAZFYC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.285"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..339)
                     /locus_tag="ACHKW5_005342"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHKW5_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(655..1299)
                     /locus_tag="ACHKW5_005343"
     CDS             complement(655..1299)
                     /locus_tag="ACHKW5_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514490.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1320..1589)
                     /locus_tag="ACHKW5_005344"
     CDS             complement(1320..1589)
                     /locus_tag="ACHKW5_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7514491.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1668..2306)
                     /locus_tag="ACHKW5_005345"
     CDS             complement(1668..2306)
                     /locus_tag="ACHKW5_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO7514492.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2291..>2601)
                     /locus_tag="ACHKW5_005346"
     CDS             complement(2291..>2601)
                     /locus_tag="ACHKW5_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFO7514493.1"
                     /translation="HTWRSYAMFLLDVMPERTAEHYRNKIAVYLRWYQTRGFPDDIPD
                     EQENDLGSRDIPSWRRICKTLIKNDFWYRTLSFSPNKPRHYERYLQRMKERRKEWGIL
                     "
BASE COUNT          628 a          713 c          528 g          732 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgttt tattaacata tagtaaaaat cacctcgccg cattttattc
      661 ttttgaagta aaatcattta ccagttcact taaagctctc ttttgttcat catcatacag
      721 agattgaatc cggaatttaa tctcttctgc aaccagtctt gcatcgggct cataaaatat
      781 ttttgttatt cgctgtttct gatgttcagg ctggtcttcc atatttcgta aaaccagatg
      841 aaataatata tcataaagtg cgcaatgcca tgaataacca tcattggtta aaataagctc
      901 ccaaacccgc tcctgctcgg caataagctg cagaacaaag gactcaacct gttctgtttg
      961 ttcgggggca acacggtata gcggagcaat cacagcaaaa gatagttcat ttaaccagga
     1021 ttccgcttcc ttctcgctcc tgaacggtgg acagttttgt attcttcgtt cgtattcacg
     1081 ggtaatctgc tccagaatat cctccggcgg tgtcagccac ttattcctga gcaatacact
     1141 ggttgatgga tgacagctgg ctatttcagc aataatttca cgtaatgtct ccgggctgaa
     1201 cttcttcagc tgattgtagt gctcccgggt aaaaccatgt tcatcgcata aaagttgcca
     1261 tgtcattgat atactgtttg ccattccttg cttcatcatt aatacctgtt acctgaatat
     1321 caacagaccg gtgtaccttt tctgaatacc tccccggacg gagtggcaat ccccattcgc
     1381 ctcagcgtgg catctgcagc tttcgccacc tcgctgatat ccagcgtttc aggtagcggc
     1441 ggggacgata tctgcgccag acaataattc cggcgcaaca acaaccactc ccgttgtgct
     1501 tctgtacatt caacatttcc cataacaaaa agccagcggt cagaagctct gcgccagcaa
     1561 ccagcagcct ccagtttctg cgcaaccatg tcttccacaa cttccggggt actctttttc
     1621 ctgctcaaaa cattaactca ctgaaataat atacatatct gacggtctca gcgaaccgtc
     1681 catgccgggc tgaaatcctc ttcctgaaac agctccgtca gcccgctaat ctgtttcaat
     1741 ctgagaactt catcctggtc catacccagc tccgtaccaa tgcgttcatt cgtccagccc
     1801 aggcgcgaca ggtccctgac aatatcagac attgaggtta tctgatgttt gcccctggcc
     1861 cggttatgtc ggatggtcgc agcaatacgt gaggcctgtc cctttctctc cggattaata
     1921 cagaccaccg gcagccagcc cttcagacgt tttcccgtac ctgcttctct gcccagcacc
     1981 tggcgatgaa aaccatccac caccaggtaa tggttctttt cctcagacac caccaccggt
     2041 tgtgtgaaac catctttctc cagtgactgc ttcagcagtt tcttctcacc cggcgccatg
     2101 acattaggat tgtaatcatt ggccaccacc tcatctgctt ttacccacag cacacagtcc
     2161 actggctcat gtgcaaaagg actgacttca tgaagcactc tgcgcaggtg attaagggcc
     2221 tgtatggtct cttcttctgg tcgttcctgg ctgaagtact gacgaatcat gttgccaatt
     2281 atctccggtg tcacagaatc ccccattcct tcctcctttc tttcatacgc tgcagataac
     2341 gttcatagtg ccggggtttg ttcggactga aggagagggt ccggtaccag aaatcattct
     2401 ttataagtgt cttacagata cgccgccagg acggaatatc ccggctcccc aggtcattct
     2461 cctgttcatc cgggatgtca tccgggaagc cccgcgtctg ataccagcgc aggtagacag
     2521 caattttatt acggtaatgt tctgccgttc tttccggcat cacatccagc aggaacatcg
     2581 catagctgcg ccaggtatga t
//
LOCUS       DAZFYC010000280         2559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFYC010000280 DAZFYC010000000
VERSION     DAZFYC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.286"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..226)
                     /locus_tag="ACHKW5_005347"
     CDS             complement(<1..226)
                     /locus_tag="ACHKW5_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7514494.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPR"
     gene            complement(226..948)
                     /locus_tag="ACHKW5_005348"
     CDS             complement(226..948)
                     /locus_tag="ACHKW5_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO7514495.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGESSGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(1023..1700)
                     /locus_tag="ACHKW5_005349"
     CDS             complement(1023..1700)
                     /locus_tag="ACHKW5_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO7514496.1"
                     /translation="MAVQISVENLSPVTYNQIPVITTELLAHLYGTKIKNISDNFLNN
                     TTRFMPGKHYFKIEKNELREFKNRPETIGLVGKNARSLILWTERGAARHAKMLETDQA
                     WDVFEKLEDCYFSQCEKNTGKQEKKPNGLSAKETDSLVWLWDYANRSQALFRELYPAL
                     KLIQSGYSGICYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLLAWERLKNKEL
                     PPSLHRY"
     gene            complement(1976..2158)
                     /locus_tag="ACHKW5_005350"
     CDS             complement(1976..2158)
                     /locus_tag="ACHKW5_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7514497.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(2155..>2559)
                     /locus_tag="ACHKW5_005351"
     CDS             complement(2155..>2559)
                     /locus_tag="ACHKW5_005351"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7514498.1"
                     /translation="ALCAHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQC
                     IQQRQTVVMLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSML
                     LIWRPFISPRRMFTTVSKAALMAIGQGVRMAA"
BASE COUNT          555 a          654 c          588 g          762 t
ORIGIN      
        1 tacggggctt taaggcgagt tttcgaatct tcagtttatc tttctgtttc tgctcctctc
       61 gtcgtcgttt cttctctgct gctttttccg ctttttcgcg ttctttactt cgtcgttcga
      121 gtgctaattg agttccgtgt tccgggcagc accaccactg atttgagaat gccgggtgaa
      181 accattcctt gcatattttg catttccttc gcgctggttt agccattaag cagcctcccc
      241 tgttactttc agcattccgt tatcgagcag ctttctggtc agccactgtt gaccacgccc
      301 ggtgattttt gtggtgaacg atatctgtat tccgtgattt gtgttgaccg ctgtttcttt
      361 cactgtgaaa tagccacgat ccatatattc ctgcattggc acattgcgcc gggagcctga
      421 agcaataagg attttgtgat cgcgcatcca cgcaaacagt ttgtttggac caataccaac
      481 aacctttgca aagtttccaa tcaaaattcc gctggactcg ccaacgcgat cggcaaactc
      541 aactttaggt gctgcgagag caagctgttt ctccagttca gccttctggt cttcaaggtc
      601 ggccgcaagg cgcaatgcct cagaaaaggt ttgtggtatt ttcgcggttg ccccttcgag
      661 ttctcgccag cggtcaacaa ggcgagcggt gaattccggc gacaactggg caacgacaat
      721 aatgctgtcg cgcttacctt gttcgccctc aaaaacgtaa gcctctacgc cacgaagtaa
      781 tcctaagtta ttgatttttt cgaaaaccac cattggggga tttcggatca cacctcgaac
      841 cgccagtcgt tcaatggatt gtttcacctt gtcatgacga cttcccacca actcagcgat
      901 ttcaatgctt gtcattttga tggcattgct atttatcagc tcattcattg tcatgtcctc
      961 tcacattgaa aattcagcaa taacaaaccc agccgaagct gggtttgtta agttgtcaat
     1021 tgtcagtagc gatgcagtga aggcggcaac tctttgttct taagcctttc ccatgccaga
     1081 aggttcgttg gtccgtctgg ctcattgata tcaacatctc gtgtgtgatt aattaaaacg
     1141 tctctcgcca ttccaataac atacgagaac tcatgaccgt agtcgtagca tatgccggaa
     1201 tagccagact gaatcagttt taatgcggga tacaactcac ggaacaatgc ctgtgagcgg
     1261 ttggcataat cccacagcca tacaaggctg tctgtttctt ttgcggaaag cccgttgggc
     1321 ttcttctctt gtttgccagt atttttctcg cactggctga aatagcagtc ttccagtttt
     1381 tcgaacacat cccacgcctg atcggtttcg agcattttgg cgtgacgggc tgctccgcgt
     1441 tctgtccaga ggatgaggga gcgggcattt ttaccaacta acccgattgt ttcgggtctg
     1501 ttcttaaact cgcgtaattc gtttttttca attttaaagt aatgctttcc gggcatgaat
     1561 cgcgtcgtgt tgttcagaaa gttatcagaa atgtttttga tttttgttcc gtaaaggtga
     1621 gccaacagtt cagtagtaat tacggggatc tggttatagg taacagggga aaggttttcg
     1681 acagaaattt gaacagccat aatgacctcg cgtttcgata atttttacct cgccaccgtc
     1741 aggtgctaat catcgtggtg gcgaactgtg cggggttagc actaccggtc gaaacatccg
     1801 gcgagccttt cggctccccc acacagcccg ccataaatcg cgaatgtgac tgtgcttagc
     1861 gcataaaaaa accgccagcg cggttatgca ccgtttcgat atccggggtg ctaatcccga
     1921 cgccagattt tgctggcgcg tgaggaatat agccccgaat aaatcatcgc gtcaatcacc
     1981 ttgttttcct cgcacgatgt cttagccacc ggatatccca caggtgagcc gtgtagttga
     2041 aggtttttac gtcagattct tttgggattg gcttgcgttt atttctggag cgtttcgttg
     2101 gaaggtattt gcagttttcg cagatgatgt cggtgatact tcttcgctgt cgcctcatgc
     2161 cgccatcctg acgccctgcc cgatcgccat caatgccgct ttggatacag tagtaaacat
     2221 tcgtcgagga ctgatgaacg gtcgccaaat cagcagcatg gagcctttgc tgtttccctt
     2281 cttctccagc cctgtcgatg gttcgataaa attaatccgt ccatcagtga taatgcgaac
     2341 ttcgtcgaca ctctccagag ccttgctgaa ccatccgact gacatatcct ctggcacaag
     2401 catcactacc gtctgtcgct gttgtatgca ctgctcagcg gctttttcca cccacggcct
     2461 gatattgctg tacggtgggt tattccagat tgcaccgtgg cttatccact cagaatttag
     2521 cgcgtcgtcg gcctcagtta gccagtgagc gcacagagc
//
LOCUS       DAZFYC010000281         2527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFYC010000281 DAZFYC010000000
VERSION     DAZFYC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.287"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..240
                     /locus_tag="ACHKW5_005352"
     CDS             <1..240
                     /locus_tag="ACHKW5_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514499.1"
                     /translation="MILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPM
                     PVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            481..>2527
                     /locus_tag="ACHKW5_005353"
     CDS             481..>2527
                     /locus_tag="ACHKW5_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7514500.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     "
BASE COUNT          587 a          670 c          816 g          454 t
ORIGIN      
        1 atgatactgg gcggtgtggc tcagatgctg gccccgaagg caaagacgcc ggagtacaaa
       61 agtacggata acggtaaaca gaacacgtac ttttcgtcgc tggataacat gattgcccag
      121 gggaacccga tgccggtgcc ttacggtgaa atgctggttg gctcccggcg aatctcccag
      181 gacatcagta cccgtgatga gggcggagac gggaaagtgg tggttatcgg gcggggatga
      241 aaataaaaaa atcccgcaga gttagcggag ctgcgggaga gaacgatgaa gattaacgtt
      301 atggagttat ttttcaggca tcaaaaaagt aatgcagcgt cattattgcg gctacaggca
      361 attgccggaa atgtgaagag tttcagaaat tttattccgt catgacacag gcaccctccg
      421 gggtgcctgt tgttttctgg cataaacaga ttcagacatc agacaggaga gggggacaga
      481 gtgggtaaag ggggcggcaa ggggcacacg ccggtagagg caaaggacaa tcttaagtcc
      541 acgcagatga tgagcgtgat tgatgcgatt ggtgagggac cgatagaagg cccggtgaag
      601 ggactgcaga gtatcctggt gaacaaaacc ccgctgacgg acacggacgg taatcccgtg
      661 atacacggtg tgactgcggt ctggcgtgcc ggggagcagg agcagacacc accggaaggc
      721 tttgagtcct ccggagctga aaccggactg ggcgtggaag tgacgaaggc aaaaccggtg
      781 acgcgcacca ttacgtccgc gaacattgac cgcctgcggg ttaccttcgg ggtgcagtca
      841 ctggtggaga ccacctcaaa gggtgaccgt aacccggcat ccgtccgcct gctgattcag
      901 ttacagcgta acggtaactg ggtgacagaa aaggacgtca ccattaacgg caagaccacc
      961 tcacagttcc tggcctcggt gattctggat aatctgcctc cccggccctt taacatccgg
     1021 atggtcaggg agacggcgga cagcaccacg gaccagctgc agaataagac gctgtggtcg
     1081 tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggccat tgtggggctg
     1141 caggtggatg cggagcagtt tggcggtcag cagatgacgg tgaactacca tatccgcggt
     1201 cgcatcattc aggtaccgtc aaactatgac ccggaaaaac gcacgtacag cggcatctgg
     1261 gacggcagcc tgaaaccggc atacagcaac aacccggcct ggtgcctgtg ggacatgctg
     1321 actcacccgc gctacggcat gggaaaacgt ctgggggcgg cagacgtgga caagtgggcg
     1381 ctgtatgcca ttgcgcagta ctgcgaccag acggtgccgg atggtttcgg ggggaccgag
     1441 ccgcggatga cctttaatgc gtacctgtca caacagcgta aggcatggga tgtgctcagt
     1501 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg gccagacgct gacgttcgtt
     1561 caggaccgcc cgtcggatgt ggtgtggccg tacaccaaca gcgatgtggt ggtggatgat
     1621 aacggcgtgg ggtttcgcta cagcttcagc gccctgaagg accgccacac ggcggtggag
     1681 gtgaattaca ccgacccgca gaacggctgg cagacctcca cggaactggt ggaagacccg
     1741 gaagccatac tgcgctacgg gcgcaacctg ctgaagatgg atgcgttcgg ctgcaccagt
     1801 cgcggtcagg cccaccgtgc cgggctgtgg gtgataaaga ccggactgct ggaaacgcag
     1861 acggtggatt tcacgctcgg gtcacagggg ctgcgtcaca cacccggtga cattattgaa
     1921 atctgtgata acgactatgc cgggaccatg accggcggac gtgtcctgtc catcgatgcc
     1981 gccagccgca ccctgacgct ggaccgtgag gtgacactgc cggagaccgg tgccgccacg
     2041 gtgaacctga ttaacggcag cggtaagccg gtgagtgtgg ccatcaccgc acaccccgcg
     2101 ccggaccgga tacaggtcag caccctgcct gatggtgtgg agacatacgg tgtatgggga
     2161 ctatccctgc cgtcactgcg ccgtcgcctg ttccgctgtg tctccatccg ggaaaacacg
     2221 gacggcacct ttgccatcac ggcggtgcag cacgtaccgg aaaaggaagc catcgtggat
     2281 aacggggcca gctttgagcc gcagtcaggc accctgaaca gcgtcatccc tccggcagtg
     2341 cagcacctga cggtggaggt gagcgcagct gacggtcagt atctggcaca ggcgaaatgg
     2401 gacacgccgc gggtggtgaa gggtgtgcgc ttcagtctgc gcctgaccag cggaagcgga
     2461 gaagacagcc gtctggtgac caccgccatc actgcggata cagagcatcg tttcagtggt
     2521 ctgccgc
//
LOCUS       DAZFYC010000282         2520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFYC010000282 DAZFYC010000000
VERSION     DAZFYC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.288"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..450
                     /locus_tag="ACHKW5_005354"
     CDS             <1..450
                     /locus_tag="ACHKW5_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7514501.1"
                     /translation="YVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQAVWDRRPSGTI
                     HHCDKGSLYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRNSWKN
                     RTEVELATLTWVDWYNNRRLLGRLDHIPPAEAEKAYYASIRNDDLAA"
     gene            1491..2363
                     /locus_tag="ACHKW5_005355"
     CDS             1491..2363
                     /locus_tag="ACHKW5_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514502.1"
                     /translation="MVKVVEDERSRIRYLERRLNENGFYLPSSLADKDYLSYQKRILN
                     TLISQGIDTLKINNFLAETDQRYFDSLPSENDLNWYRNDARASLWLTCELYEMIKING
                     YENTLTCLSPESLPSHHSVRVDAIRRCIDNWPFILYTPSNYLNQKSIEWTTLLEKDDI
                     FREVKARNVDICSWLKKYIQEKTNISLNYVCGESSEEIMAWCYASYFTWKKNNQNSPD
                     SVELFTRKFKSAWATQKNRIKNRVDKKLRPLNVNISQEAYDKLRKLSMNEGISNDRVI
                     ESALDMIYRSKIKK"
BASE COUNT          816 a          515 c          541 g          648 t
ORIGIN      
        1 tatgtggcgt tcatcattga tgtgttcgcc ggatacatcg tgggatggcg ggtctcatcg
       61 tccatggaaa cgacattcgt gctggatgca ctggagcagg cagtatggga ccgtcgtccg
      121 tctggcacta tccatcactg tgataaaggt tctctgtatg tatcgctggc ctacacgcag
      181 cggcttaagg aagcaggatt actggcatca acagggagca caggcgactc gtatgacaac
      241 gcgatggcgg agagcatcaa tggtctttac aaagcggagg taatacaccg taatagctgg
      301 aaaaaccgga cagaagtgga actggccaca ctaacgtggg tggactggta taacaatcga
      361 cgattgctgg gaaggctgga tcatatccct ccggcagaag cagaaaaagc ttattatgct
      421 tccatcagaa acgatgatct ggcagcctga gttcacaaat aaaacaatct ccaggaaacc
      481 cggggcggtt caaatttcgc agagggctgg cttctgatgc tgacgctaac gtgcttttcc
      541 cacctgctaa ccagaaagta atctgcccag cataacagta accccatcag cttaaaatca
      601 ataacgccct gtaaaacctg aagtaacagt ccgggaactc gtatcatgct aacatatcag
      661 tgttgtgaag cagtcgacaa cgagcttcag acggtacaca cgtcagttca tgcagaaata
      721 gcggccatat caggtctggc tgtcagcgaa ccacgcagcc tgagtacatt gttgtaaatt
      781 aaacgactgg tgatttttaa attttttctt tgccaccacc gtgaactaat accgaaaaaa
      841 cagtagtctc aggaagcgag ttctgaaagt cagctttatt aaaataagct attagaccgg
      901 gtaagtaatg tcaacgaatg aaatgttatg ataattctgg ataccatatc tgtaagagtg
      961 attaatgagg aaagtgatga cttttaatta ttttatctcc ggtaacgtaa aaccagactt
     1021 atcgtaaatg aaattatgaa gtcctctgtt aacgtcatgg agagacgcat caaaatacag
     1081 gatacaatga ttctcacaat gaaactcatc gtctctggtg aaattcatat caacatttac
     1141 cttaataacc tgacgcggga ttaactgtgt tgtttaaggc aaagggggga gttgcccgaa
     1201 ggcgaaaacg aaagtaagtt aataataaca ggatgagagg agaaagcgct cttcccggaa
     1261 aataaaaaga gcggttcact cctgtgaatg cacttcaacc gtcctggcaa agaatgtact
     1321 gttataaaaa actcccccag tcagcagaga caaaaagata acatgaggta cctcccaccc
     1381 ccttaattta tacacgtgaa atgaactgaa atttatgaga aaatcatgct aaagatgctt
     1441 tgattttact taaaatcacg tgtgattatc ctgactgaag gagagatact atggttaagg
     1501 ttgtggaaga tgaaagaagc agaatacgat atcttgaaag acgcttaaat gaaaatgggt
     1561 tttatttacc atcaagcctg gccgataaag attatttatc gtatcagaaa cggattttaa
     1621 acactcttat ttcacagggt attgacaccc taaaaataaa taattttctg gctgaaacag
     1681 accagcgtta cttcgattcc ttaccatctg agaatgatct taactggtat cgtaatgatg
     1741 cccgcgccag tttatggctg acttgtgaat tatatgaaat gattaagata aacggttatg
     1801 aaaataccct tacctgttta tctccggagt cactaccttc tcaccacagc gtgagggtag
     1861 atgccatacg caggtgtatt gataactggc ctttcatttt atacacacca tcaaattacc
     1921 tgaaccagaa aagtattgaa tggaccacac tgctcgaaaa agatgatatt ttcagagagg
     1981 ttaaggccag aaatgttgat atctgctcat ggctcaaaaa atatattcag gaaaagacga
     2041 atatttccct gaactatgtc tgtggagaat catctgaaga gattatggcc tggtgttatg
     2101 ccagttactt cacatggaaa aaaaacaacc agaattcacc agactcagta gaactgttta
     2161 cccggaagtt taaaagcgcc tgggctaccc aaaaaaacag aataaaaaac agggttgata
     2221 aaaagctgcg ccccctgaac gtaaatattt cacaggaggc atatgacaaa ctgagaaaat
     2281 tatccatgaa cgagggtatc tcaaatgatc gggttattga aagtgctctg gacatgattt
     2341 accgtagtaa aattaaaaaa taaaaccatt caaaataatt tcagacagat attatctccg
     2401 tgaatccccc gccacctttc cggtgtgcgg ggtcttgtct tttttcaccg ggaatacatg
     2461 tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc
//
LOCUS       DAZFYC010000283         2515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFYC010000283 DAZFYC010000000
VERSION     DAZFYC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.289"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..393
                     /locus_tag="ACHKW5_005356"
     CDS             <1..393
                     /locus_tag="ACHKW5_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514503.1"
                     /translation="MSRHIRKMQKKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNII
                     EIGYNIINTDLVIGEKIHFRTLSNRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNNET
                     KSTIDNYISKIKDNHNTPWLLNNENVQE"
     gene            complement(1263..1403)
                     /locus_tag="ACHKW5_005357"
     CDS             complement(1263..1403)
                     /locus_tag="ACHKW5_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7514504.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1705..1968)
                     /locus_tag="ACHKW5_005358"
     CDS             complement(1705..1968)
                     /locus_tag="ACHKW5_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO7514505.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2392..>2515
                     /locus_tag="ACHKW5_005359"
     CDS             2392..>2515
                     /locus_tag="ACHKW5_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO7514506.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT          798 a          574 c          452 g          691 t
ORIGIN      
        1 atgtcacgac atataagaaa gatgcaaaaa aaactaaaac agacgtctgt gcctgatttt
       61 atttattatt caatagaaaa acacaacaaa tatcccatat atgtattaca catgccatca
      121 aacaatataa ttgaaatagg ctacaatata ataaacaccg atctggtcat tggcgaaaaa
      181 attcatttca ggacactaag caatcgttct ttatatttta acctcaccca acccccatta
      241 atcgccacaa taaaagatga tgtgttttgc actcttcatg attactataa ccacaataat
      301 gaaacgaaat ccactattga caattacata tcaaaaataa aggataatca taatacccca
      361 tggttattaa acaacgaaaa tgttcaggaa taaaaaaaag agacaattcc cgagataaaa
      421 caaaagagca taaacatatg aaccggaaat tctatttcaa aaatatcctg tggggaatgt
      481 gccccattat aatatccata ttgcatctga tatatgacac accaacagaa gcatcgataa
      541 aagttgactt atttctgata ctcagtggcc tggcccttcc ttgttcaata tacctgacaa
      601 aaacaatcac actaaaatac acatccatat cattctggaa aaaacctctt ttctggtctt
      661 atggtatata tcattttcat cttgtttata taatgacatg tatagtttta gcaatacctt
      721 ctgcaattat cgttttattc tttcactttt atcaaaacat caccagaaac tggaagtgac
      781 aaccgcaata atgaacataa aaaggcaaat cagaatggca gtaaatcaat aaagtaccat
      841 ttcatatgaa tcatcagaaa aacggtggac gcgacatcca gcacgaggca aaacacagca
      901 acaaaataag ccggatatcg cgccctgtac ttttctaact caactcccgg cattgtgcaa
      961 gttttaatcg caggtggatt cagagccacg cccccattga ttcagcctga agacacgtat
     1021 atccttggag aaagcaggaa tctcattgct gtgaaattcc ttacagaatc ttcagcccca
     1081 ttttatgggg ctttttttgg tgccccacgc tgttaccaca ctttcgggat gcgaataaaa
     1141 aagcccacag tttttacact gcggactttt gttttttctt tgtgatttgt gtgcaagggt
     1201 gcgactttcg accgaacctt tggcaaccgg atagcgggga ctgttgtccc cgctgcggtt
     1261 tcttacggtt tacactgcaa gaacgccgca atttccgacc acatgaagct taccctgaat
     1321 tcacacggtg atgcgtgtaa cacccagacg gtcagcaaca tggtcaggca taacatgccc
     1381 acaatgatcg ttttttgcgg catagcactt gctcctttta cagagaggcg ctaaccttcc
     1441 acttgctgaa ggtagatggt cagggcctcg ggttaacgtt taagttgact cggggccttt
     1501 ccacatccgg ccttcaggta atccttccag ccgtcagccg aaaggcaccc gcgcgtaatg
     1561 tactggtttt cagagaggtc cgcaatcttc cggtgacgag atcatctgca cacgccccgg
     1621 tgggacgggc taacggtata ttcaggtgca ggatctctct gtctggtgcg tttttaaatt
     1681 gtcaaacgca ccctggcggc aaaatcagcc accttttttc gcatcaacaa aacgcccaaa
     1741 acgctgggta aagcgtgcaa cattaccttc tgaagcgaca gttctcagtt tacctgtata
     1801 aaacggatgc gacttagaag aaacgtcaat tgtaacatat gggtaagtta caccatccag
     1861 ttcaattacc cggtcagttt tgatcgtcga accgatcctg aagtactcat caatactggt
     1921 atcgtgaaat actactgtac ggtattccgg atggatattc ggtttcacaa cagttttccc
     1981 cctttaagtg aaaattccgc ataaaggtac atattcagac ggtcagattc aactgtctgt
     2041 tcttataacc tcactctctt tcagtattac aatatgttat ataattataa actggacttc
     2101 ccgaaaaaat ccattaaata agaagcgagc cgttgcggag gcttgctctc tcctttaacc
     2161 tcatgtgcaa tatccgtaac gcgctcacat aaatcatcat ctgataatat aacaacaccc
     2221 attattttat tctgacctaa aaacttctta tgaaataaac gtttacccga cgtatctgat
     2281 cccgctgttt ttacctgtat cctttaacat acttgagaag cccgctgaaa aaatataccg
     2341 taaaggcggt tctctgtctt ccataaacct gtcggataag aagggcatta aatgaaaacc
     2401 aaaaaagtta tgatgattgc actggtcagc tctgcgctgg ctctttcagg atgtggagcc
     2461 atgggcacag cgatcaaaaa acgaaatctg gaagtaaaaa cccagatgag tgaaa
//
LOCUS       DAZFYC010000284         2411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFYC010000284 DAZFYC010000000
VERSION     DAZFYC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.290"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..1123
                     /locus_tag="ACHKW5_005360"
     CDS             92..1123
                     /locus_tag="ACHKW5_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFO7514507.1"
                     /translation="MFKNKVLLITGGTGSFGNAVLQRFLDSDIGEIRIFSRDEKKQDD
                     MRKKYASDKLKFYIGDVRDYSSVLTATRGVDFIYHAAALKQVPSCEFYPMEAVKTNVI
                     GTDNVLEAAIANKVSRIVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNLPKDITICA
                     TRYGNVMASRGSVIPLFINQILEGRPITITDPCMTRFMMTLDDAVDLVLHAFEHGTNG
                     DIFVQKAPAATIDTLTKSLLKLTKQHSHPINIIGTRHGEKLFEVLCSREEMLVAEDQG
                     NYYRIPSDKRDLNYEKFFDKGTKEIQFVEDYNSHNTRRLDVDEMVALLRKLDYINKIE
                     AGEKADPDA"
     gene            1116..1985
                     /locus_tag="ACHKW5_005361"
     CDS             1116..1985
                     /locus_tag="ACHKW5_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase family protein"
                     /protein_id="HFO7514508.1"
                     /translation="MRKKILIVGANGMLGSSLLRYFSSIGDYEVLGTTRSMVVAKQLE
                     QKHNVKIIDNVDVIDFKRLETVVVEHKPNIVFNCVGIIKQLDAAKNNILSIEINSLLP
                     HKLAQLCSAHSAKLIHFSTDCIFKGTKGNYVEDDESDAIDLYGKSKFLGEVEYNGHLT
                     LRTSIIGHELGSNHSLVDWFLSQKKSVKGFTNAIFSGLPTCYMAEVIHKYVLPNNLAG
                     LFHLSVEPISKYDLLNIIKIVYGVSTDIEPTNEFKIDRSLNSTLFRNKTNFVPESWDK
                     LIEKMKDEYNKYF"
     gene            1963..>2411
                     /locus_tag="ACHKW5_005362"
     CDS             1963..>2411
                     /locus_tag="ACHKW5_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFO7514509.1"
                     /translation="MNTINISNSKIRVVTVVGTRPEIIRLSRVIAVLDEYTEHFLVHT
                     GQNYDYELNEVFFNELEIRKPDFFMNAAGQNAAETIGNVIIEADKIFDKLQPEALLIL
                     GDTNSALVSIAAKRRKIPIFHMEAGNRCFDYRVPEEINRKIVDHISD"
BASE COUNT          795 a          363 c          473 g          780 t
ORIGIN      
        1 taatacttta tctgaaagtg aaaatagcta tatgtattgt ccgaaaacaa gctgggcaga
       61 tttcttcgag ttaatctaat tgaggtatac gatgtttaaa aataaggtac ttctcattac
      121 tggtggcaca ggttcctttg ggaatgccgt tttacaacgc tttttagatt cagatattgg
      181 cgaaattcga atctttagtc gtgatgagaa aaaacaagat gatatgcgta aaaaatacgc
      241 aagtgataag ttaaaatttt atatagggga tgtgagggac tatagcagtg ttttaacagc
      301 gactcgtggg gttgatttta tttatcatgc agcagcatta aagcaagtac cttcatgtga
      361 gttttatcca atggaagctg tcaagactaa cgttattgga actgataacg tacttgaagc
      421 tgcaattgca aacaaagttt cacgtattgt ttgtttgagt accgataaag ctgtttatcc
      481 aattaatgct atgggtacat ctaaggctat gatggaaaaa gttatcgtag ctaagtcaag
      541 aaacttacct aaagatatta ctatttgtgc gacgcgatat ggaaatgtaa tggcttcccg
      601 tggttcggtc atccctctgt ttattaatca aatacttgaa ggacgcccaa tcactatcac
      661 cgatccgtgt atgactcgat tcatgatgac tttggatgat gctgtagatc ttgtattaca
      721 tgcttttgaa catggtacta atggtgatat ttttgttcaa aaggcaccag ctgcaacaat
      781 agatacactt accaagtcat tacttaagtt aactaaacaa cattctcacc caattaatat
      841 cattggcact cgccatggtg aaaaactatt tgaggtactc tgcagtcggg aagaaatgtt
      901 ggttgcagaa gatcaaggta attactatcg tatacctagt gataaacgtg atctaaatta
      961 tgaaaaattc ttcgacaaag ggactaaaga aattcagttt gttgaagact ataattctca
     1021 taatacccgt cgtttagatg ttgatgaaat ggttgctctt cttagaaaat tagattacat
     1081 caataaaatt gaggctggtg aaaaggcaga tcccgatgcg taaaaaaatt ctaattgttg
     1141 gtgcaaatgg catgttaggt agtagtttac tacgctactt ttcatcaatt ggagattatg
     1201 aagttcttgg tacgacaaga agtatggtcg ttgcaaaaca acttgagcaa aagcacaatg
     1261 tgaaaattat tgacaacgtt gatgttattg attttaaacg attagagact gtagtagtag
     1321 agcataagcc aaatattgtt ttcaactgcg ttgggataat taaacaactt gatgcagcaa
     1381 aaaacaatat attatctatt gaaattaact cattacttcc acataaatta gctcaattat
     1441 gttcagctca tagtgctaaa ctcatacatt tttcaacaga ttgcattttt aaaggtacca
     1501 aaggtaatta tgttgaggat gatgagtctg atgcaattga tttatatggt aaatctaagt
     1561 tcttgggtga agttgaatat aatgggcatt taactttgcg gacttctatt attggccatg
     1621 agttgggatc aaatcatagt cttgttgact ggtttttatc acagaaaaaa tcggtgaaag
     1681 gatttactaa tgcgatattc tcaggcctcc caacttgtta tatggcagag gttatccata
     1741 aatatgttct tcccaacaat cttgctggtt tatttcattt aagtgtagag ccaattagta
     1801 aatatgattt gttaaatatt attaaaatag tatatggagt aagtactgat atagagccaa
     1861 ccaacgaatt taaaatagac cgcagcttga attctacgct atttcgtaat aagacaaatt
     1921 ttgtcccaga atcttgggat aaattaattg aaaagatgaa agatgaatac aataaatatt
     1981 tctaattcaa aaatacgggt tgttactgta gttggcacta ggcctgaaat cattcgttta
     2041 tcgagggtaa ttgctgttct cgatgagtac actgaacatt ttcttgttca taccggacaa
     2101 aactatgatt acgagcttaa tgaggtattc tttaatgagc ttgaaatcag aaaacctgat
     2161 tttttcatga atgccgcggg ccagaatgct gctgagacca tcggaaatgt aattattgaa
     2221 gctgataaga tatttgataa attgcaacct gaagctttac ttattcttgg tgatacaaat
     2281 agtgctctag tttccattgc agctaaaaga cgaaaaattc ctatctttca tatggaagcg
     2341 ggtaaccgat gctttgatta cagagttccg gaggaaataa ataggaagat tgttgatcat
     2401 atttcagata t
//
LOCUS       DAZFYC010000285         2403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFYC010000285 DAZFYC010000000
VERSION     DAZFYC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.291"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..287
                     /locus_tag="ACHKW5_005363"
     CDS             <1..287
                     /locus_tag="ACHKW5_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO7514510.1"
                     /translation="NIHIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSFDRLKSREI
                     TFQQYRENLAKAGVFRWVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            578..871
                     /gene="bor"
                     /locus_tag="ACHKW5_005364"
     CDS             578..871
                     /gene="bor"
                     /locus_tag="ACHKW5_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO7514511.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(962..1144)
                     /gene="rzoD"
                     /locus_tag="ACHKW5_005365"
     CDS             complement(962..1144)
                     /gene="rzoD"
                     /locus_tag="ACHKW5_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO7514512.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPRPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1361..1858)
                     /gene="rrrD"
                     /locus_tag="ACHKW5_005366"
     CDS             complement(1361..1858)
                     /gene="rrrD"
                     /locus_tag="ACHKW5_005366"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7514513.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(1858..2073)
                     /gene="essD"
                     /locus_tag="ACHKW5_005367"
     CDS             complement(1858..2073)
                     /gene="essD"
                     /locus_tag="ACHKW5_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO7514514.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          692 a          557 c          494 g          660 t
ORIGIN      
        1 ataatattca cattgtgtta gaaaacgata acactgtgtt aataaaagga cttaaaaagg
       61 ttgtaaatgt taaattctca agaaatacgc atcttataga aacgtccttt gataggttga
      121 aatcaagaga aatcacattt cagcaataca gggaaaatct tgctaaagca ggagttttcc
      181 gatgggttac aaatatccac gaacataaaa gatattacta tacctttgat aattcattac
      241 tatttactga gagcattcag aacactacac aaatctttcc acgctaaatc ataacgtccg
      301 gtttcttccg tgtcagcacc ggggcgttgg cataatgcaa tacgtgtacg cgctaaaccc
      361 tgtgtgcatc gtttttaatt attcccggac actcccgcag agaagttccc cgtcagggct
      421 gtggacatag ttaatccggg aatacaatga cgattcatcg cacctgacat acattaataa
      481 atattaacaa tatgaaattt caactcattg tttagggttt gtttaatttt ctacacatac
      541 gattctgcga acttcaaaaa gcatcgggaa taacaccatg aaaaaaatgc tactcgctac
      601 tgcgctggcc ctgcttatta caggatgtgc tcaacagacg tttactgttc aaaacaaaca
      661 gacagcagta gcaccaaagg aaaccatcac ccatcatttc ttcgtttctg gaattgggca
      721 gaagaaaact gtcgatgcag ccaaaatttg tggcggcgca gaaaatgttg ttaaaacaga
      781 aacccagcaa acattcgtaa atggattgct cggttttatt actttaggca tttatactcc
      841 gctggaagcg cgtgtgtatt gctcaaaata attgcatgag ttgcccatcg atatggtcag
      901 ctctatctgc actgctcatt aatatacttc tgggttcctt ccagttgttt ttgcatagtg
      961 atcagcctct ctctgagggt gaaataatcc cgttcagcgg tgtctgccag tcggggggag
     1021 gctgcattat ccacgccgga ggccgtggtg gcttcacgca ctgactgaca gactgctttg
     1081 atgtgcaacc gacgacgacc agcggcaaca tcatcacgca gagcatcatt ttcagctttc
     1141 gcatcagcta actccttcgt gtattttgca tcgagcgcag caacatcacg ctgacgcatc
     1201 tgcatgtcag taattgccgc gttcgctagc ttcagttctc tggcattttt gtcgcgctgg
     1261 gctttgtagg cgattgcgtt atcacggtaa tgattgaccg cccatgacag gctgacgatg
     1321 atgcagataa tcagagcgga tataatcgcg gttactctgc tcactgttgc ccccacaaac
     1381 agacttcacg ctcaatctca cgacgagtca tcaggccttt ccattgctta ccgccagcgt
     1441 atgtccagcg acgcagctga tcacatgcgc ctttgatatc gccctggttt attttgcgaa
     1501 gaagcgtcga tgttctaaaa ttgccagcac ccacattgta aacgaatgag taaagagcgc
     1561 cgcgcgttgt ttccggtata tcaactttga tatacgggtt aatttgtctg gcgacagtgg
     1621 caaggtcttt attcaagagt gctttgcatt ctgctttggt atacgtttta ccgagcatga
     1681 tgtcttttcc ggtgtgtccg tgacatacag tccatacacc aacaatatct ttgtatggta
     1741 tgtagctgac accttccaga ccatcgttac cacttgggcc agtgattaac actgatgcta
     1801 tagcaattgc tccgccacca atagcagcag caacggcttt tcgtaatgat ggaggcatta
     1861 ttcacctctc gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta
     1921 cgtcagcagg ccaaatacca ggctacccag cacacctatt gctgcccact gtgagggagt
     1981 gactttatcg agcagctgta aaaaccagta accggcacta cctgctgagg tgccataggc
     2041 gactccagtt gttaacttat ccatgaattt cataacccca cctcgcagac aaagcgggtg
     2101 taaattgagg gaatacaacg tatcgcaaaa aagcagaaac gtaactgact ccgagtcagt
     2161 gaataactca ggtattgagt tatcagctaa tatcgagact caaaaaatgg aaaaaccagc
     2221 tcgacggcgg gtttaagctg tgtgacgaag taaccactct taacagcata accaattttt
     2281 tacgtacgta aaccactgaa tgatatttat gagaatgcta ccgagtgttc aaaacatcac
     2341 cacaaataca taagaaaacc tcaacaaata accaataaat aatttccagt gttattttta
     2401 gcc
//
LOCUS       DAZFYC010000286         2390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFYC010000286 DAZFYC010000000
VERSION     DAZFYC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.292"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..802)
                     /locus_tag="ACHKW5_005368"
     CDS             complement(113..802)
                     /locus_tag="ACHKW5_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO7514515.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHKW5_005369"
     CDS             complement(817..939)
                     /locus_tag="ACHKW5_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO7514516.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            1278..2237
                     /locus_tag="ACHKW5_005370"
     CDS             1278..2237
                     /locus_tag="ACHKW5_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO7514517.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          779 a          513 c          430 g          668 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagttc
     1021 ttatattgct atatagaata gccattacta atgcatttaa atttaataaa ataaaaatta
     1081 taaaaaacat aaaacaccac gtaaacacac ttaataaaaa caccgttaca ttaaaagata
     1141 ataaaaaccg caataaaaaa cgaataaatc aattgtctca cgcaattata aaacatcata
     1201 ttgattacgc accttgtatt acaaactcat gtatgtaaaa tacgcgcacc attcaaaaaa
     1261 aaggaagaca ataacatatg aaaaaaagtg tcatcgctgg cgtctttatt gctctgtcat
     1321 ttaccacgtg ttcagctatc gcgaacagcc ttgcattatc attagcaaat gatgatgcag
     1381 ggaagtttca accaatactt aatgatattt atggcaataa acatgaaaac agagatgatt
     1441 actcacaagg cttatttctg ggatatagcc acgatatctc agactcgagc caattatctc
     1501 tccatattgc gcaagatatt tactctccat caggcagtaa taaaagacac aacacagctg
     1561 taactggaga cagagctttt agtgcataca ctcacactgg tattgaatgg aactcccttg
     1621 cgaatgactg gattcgctat cgattaggta ctgacatagg tgttgttggc cccgacgcag
     1681 gcggtcagaa agtacaaaat aaagctcatg agattattgg ggcagaaaaa tatcatgcat
     1741 gggatgatca aatagagaat cgctacggtt atactgtaaa agggatgcta tccatgacac
     1801 caagtatgga tattttaggt gctaatgttg gattataccc tgaagtttct gctgttactg
     1861 gaaacttatt tcaatatgta gcatatggcg caaccattgc cattggtaat gataaaacct
     1921 tcaattcgga taatggcttt ggtctgctgg ctccccgtgg tttaatgcat atgtccgata
     1981 caagcggatt caaatacaag atttttgcag gtatggaaag acgagatgtc aatcgcaact
     2041 atactctcga aggaaaaaca atacagacga aacaaacaac agtatcgcta aacaaaactg
     2101 ttgatgaata tcaagttggc gcaacaattg ggtatgcacc tgtagccttc acactagcat
     2161 ttaataaagt aacatcagaa ttcaagacag gggatgacta ttcatttata aatggagcaa
     2221 tcaccttctt tttttaactg aattgaattc aatcaaaata acataagtcc aacaaaaaca
     2281 taaagtgcga aatgaatgcc agctccattt atttcgcact ataaaagatt aaaagttgca
     2341 ataaaataat aaaatgactc agttacgaaa accaataaac tgtggccagt
//
LOCUS       DAZFYC010000287         2375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFYC010000287 DAZFYC010000000
VERSION     DAZFYC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.293"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(3..926)
                     /locus_tag="ACHKW5_005371"
     CDS             complement(3..926)
                     /locus_tag="ACHKW5_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO7514518.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1037..2221)
                     /locus_tag="ACHKW5_005372"
     CDS             complement(1037..2221)
                     /locus_tag="ACHKW5_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO7514519.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANSQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT          733 a          558 c          544 g          540 t
ORIGIN      
        1 tctcactcat gccgcgccgg acgatttatt tttctggcgc ggcgacggga atccattgaa
       61 atcaaaatca ccgatgacag aatcagcagc gttcccagcc agtccggtaa tgtgaacgtg
      121 atccccagca acagcaaaga gagtagcgcg ctacttaatg gttctgcaca gctcaaaatg
      181 ctggcttttg gaccgccaat taattgtgct cctttcaggt acagactaaa tgtcagggac
      241 gtaccaatga ccaccaaata aaaaaacgcc agaatcaaac tgccgttaac gacaaagttt
      301 gttccttgtc tggcataaaa aggcaacaga atcagaccgc caatcagcat actccagccg
      361 acgactggta acgtgccata gcgggcaatt agcgttgagg gataggtggt atagaatgca
      421 gcagcaaatg ccgaggcaat gccccagaac aacgcggcag gagagatcga taatgacgtc
      481 ggattaccgt gtgtcaccaa taaaaaagtc ccgaccagcg atgtcaaaat agcgcagaaa
      541 accagaatgc ccgggcgcga tttacgcacc agtgagaacc aggcgacgat aatcgtcggt
      601 gagaggaatt gcagcaccgt tgccgtggct gcgttcgatt tttcgatggt tagcaaaaaa
      661 gtgagctgta cagttagcgc gccaaccacg gaaaaaatca gcaggctaat ggcatcttta
      721 tgattgttaa taatagaaaa gattttatcg ccatgaacaa atgacagcgt cagtagaatc
      781 aaaccggcga atatcaaacg cgtcatagtc aaaaactgcg acgacatctg gctttgctcc
      841 atgatgtatt gcgcgcagac ccctgaactt ccccacaaca cggcggcaat cagaacgttc
      901 agcatcccct ttctggtgga acccattttt ccctctgcat actgttttgt cgtttatgta
      961 tgcaggcatg atagcaaaaa tggcgaggat ggctctgttt gcgccacgcc gatttttctt
     1021 agcccagact ggtgagctaa atatctttaa taaacagcag gcaaatcatc gcaataccca
     1081 acatccctat cgccagccag aacaacgcat ggtagctcca gatttcaacc ataattccgt
     1141 caacagagcc ggcgataatc cagccgacgc gtgaagtatt tgcatataac gttgtcgcag
     1201 agcctatttt ttcaggcatc aagtcctgaa aataaagcat gccgatacca cagagaatac
     1261 caaggaagat ggcattaaga atttgcaatt ccagctcaac cgccggagtc gtcgccatga
     1321 gtacgctggc gtaaaaacac attccactca cgatagcaat gagcattaat agccgcttgc
     1381 caatacgttt catgtaatag cctgcgatta acatcatcgg aatttccaga ccggcagcga
     1441 taccaatcat ttctccagcc agtttatcgg ttagatgcag ttcatcaata ataaatagcg
     1501 gcatatttat catgtagaga ttattcgccg cccacatcat tgaacagacc acaaaaagta
     1561 gccgcgtatc ccgcttcctg tgggtggagg gtaaaatttc tacgggttgg gtaacgacag
     1621 gaatatttct ttgtattgat ggcaaaaaca accagactat cagtccgcaa acaacaaatg
     1681 cgatggcagc ggtgagatac atcactttaa aactaaatcc cattgccagt tcataagcaa
     1741 gcggtggccc gataacccag gcaagcgaga tctgagcacg taaaaatgta ctgaacataa
     1801 ccgtctcacg gcctgttctg tcggcgtgtt cacgggcgag ggcgaacatt tgcgagtttg
     1861 cggtagaagc aaaactactc agaagtacgc ccgttgagag cagaatgaag tagttgcgat
     1921 tccacgcaaa aagcgtgcag gccagcactc caaataagca acatagcaga atcagtaatt
     1981 tacggtcgcc ttgtttatcg gagtgccttg ccagaaattg actgaccaga attcccataa
     2041 tagcgctacc ggtgaagaaa aaacctacca ttataggacg ggctttcagt tcatctgcga
     2101 ggaatatact tagggtagga gtctgaagag cgcccgcaat acccgtcaga aaggcgacaa
     2161 gtaaaaatgc cgcggcagtg agatcaagta tttttgatgg agtggtagcc gttttttgca
     2221 ttcttctgaa tcaccgggcg aaaaaatata actgagttac aaataagtac cagtcgaatg
     2281 agtattgtat taagaatttt tgagtatcgc tgatacaacc agaaagcaat gctagcgcaa
     2341 tattataggg ccagcaattg agattattag caaaa
//
LOCUS       DAZFYC010000288         2371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFYC010000288 DAZFYC010000000
VERSION     DAZFYC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.294"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..276
                     /locus_tag="ACHKW5_005373"
     CDS             <1..276
                     /locus_tag="ACHKW5_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7514520.1"
                     /translation="VNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGGATVTPKLNG
                     VAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLMLEINAPAA"
     gene            569..784
                     /locus_tag="ACHKW5_005374"
     CDS             569..784
                     /locus_tag="ACHKW5_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7514521.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            789..1133
                     /locus_tag="ACHKW5_005375"
     CDS             789..1133
                     /locus_tag="ACHKW5_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO7514522.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1184..1717
                     /locus_tag="ACHKW5_005376"
     CDS             1184..1717
                     /locus_tag="ACHKW5_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7514523.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGTTRVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDLKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1873..2055
                     /locus_tag="ACHKW5_005377"
     CDS             1873..2055
                     /locus_tag="ACHKW5_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514524.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            2068..2199
                     /locus_tag="ACHKW5_005378"
     CDS             2068..2199
                     /locus_tag="ACHKW5_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514525.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            2207..>2371
                     /locus_tag="ACHKW5_005379"
     CDS             2207..>2371
                     /locus_tag="ACHKW5_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7514526.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     ELANVTITDMQ"
BASE COUNT          607 a          485 c          695 g          584 t
ORIGIN      
        1 gtgaatctga tgcatcacag gaaagccgga aacacgaagc tgggggagtt cggcgattac
       61 ggtaacgact ggcagacgct ggagctggtg ttcaccgccg gcggtgccac ggttactccg
      121 aaactgaatg gagtggctgg cccggcattc caggttataa aagacagtct gacactggga
      181 ctgaatgcgc tgacgctgac ggatgttaca aaaaatgcag cgtatggcgt tgagatagaa
      241 agtctgatgc tggagataaa tgcaccggca gcataataaa aaaacagcca gtactgactt
      301 tcgtcggaga agtactggct aagaaggata gttgggtttc acatgatact tatatctggc
      361 agtacatttt ctgacagaca gtgatgggtg ttgtcaagat attgtgtcat ttataacctg
      421 aatcaggggg gggagccgga atgttatctg gcatttttag cagagcctga atgccataat
      481 cacggctccc ggcgttggcc gtcagtgggc gacactggcg gctttttgtt ttcctttact
      541 ttcattttct gtcggcggtg acggagacat acatcagatg gaaaaaatca caacaggtgt
      601 gtcatacacc acgtcagcgg tggggacggg atactggtta ctgcagctgc tggacaaagt
      661 ctctccgtct cagtgggtgg caatcggtgt gctggggagt ctgctgtttg gcctgctgac
      721 gtatctgact aacctgtatt tcaaaatcag agaggaccgt cgtaaggcgg cgcgggggga
      781 gtaaagcgat gaagaaaaaa tacgaactgg gtgttaaagg gataaataat tacccggata
      841 agattactgt tactgtggca ctggaaattg gtgggtatcc gtcactgttg ttgccagatg
      901 tggcgattag tcttgaccgt actgaaggtg ccacgctgga gttttacgaa gctgaggcga
      961 aaaagcaggc gaagcagttt ttcatggatg ttgctgccgg gttatgtgaa ggggatggtc
     1021 cgttgccgga aaagcgcccc gtaattttag aggcgcagga tgtgttgata acctacagag
     1081 gaaaactacc gggaataatt actggttctc tgaagactcc accgctggcc tgaagactta
     1141 acatatccag ggatttgaaa tcgataaacc ctgataaata tccatgaacg caaaaatcag
     1201 atacggcctg tcggctgccg ttctggcgct gattggtgca ggggcgtctg cgcctgaaat
     1261 cctcgaccag tttcttgacg aaaaagaagg taaccacacc acggcatacc gtgatggtgc
     1321 ggggatctgg accatctgcc gcggtaccac ccgagtggat ggtaaacctg tcgtcccggg
     1381 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgaacgtg ataaggcgct
     1441 ggagtgggtg gagcgcaata ttaaagtacc gctgaccgaa ccccagaagg cgggtatcgc
     1501 gtcattttgt ccttataaca ttggccccgg taagtgtttc ccgtctacgt tttacagacg
     1561 gattaatgca ggtgatctaa aaggtgcctg cgaagcgatt cgctggtgga ttaaggacgg
     1621 tggcagagac tgccgtattc gctcaaacaa ctgctacggt caggtttcac ggcgtgacca
     1681 ggagagcgcg ctggcgtgct ggggaattga cagataagca gaatattttg ctgaaaaatg
     1741 cggtttgctc acactggcgg ataacacgaa atcctgcgaa ctggcaaaaa ctaagtgaat
     1801 aaaagtaaaa ccccgtttgt tggccgcaaa cggggttttg tgtttctgac cttgagtaag
     1861 gcaagggaga acatgggaaa gtataaacga attctgttga gattgactat gaaaaacggc
     1921 cttgaactga aagcgcctgt aactgatgac gtcagcagag cgctggcttt tgctattaag
     1981 tgggtggcgg tcggtattgc tgtgtctccg atgctgtatg ggctggcaaa actggtcatt
     2041 gcgttgaaat cgtgaagagg attaagcatg tcagacaagc tcataacgct ggcgaagatc
     2101 ctctgtgtaa tcgtcggcat ttcattttca ctaatgctgg ttgctatttg cttttccact
     2161 gcctggcgcg tcttaacttt gtcatggctg gtggggtgag ggggatatga accgtgttct
     2221 gtgtgtggtg attattgtcc tgctggtagc ctgtggtgtg cttagtctgg ggctgaatca
     2281 ttaccgcgat aacgccatta cctacaaagc gcaacgcgat aaaaaagcca gtgagctgga
     2341 gctggcgaac gtgacaatta ctgatatgca g
//
LOCUS       DAZFYC010000289         2367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFYC010000289 DAZFYC010000000
VERSION     DAZFYC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.295"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2262
                     /locus_tag="ACHKW5_005380"
     CDS             <1..2262
                     /locus_tag="ACHKW5_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7514527.1"
                     /translation="LTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSE
                     DEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDK
                     GRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGM
                     KLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRA
                     YGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPE
                     LHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQ
                     HRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEMTWYGWD
                     GDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHG
                     VVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFY
                     HCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYY
                     NRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDS
                     YGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAI
                     TYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVM
                     TLACRTWQECWVN"
BASE COUNT          578 a          597 c          745 g          447 t
ORIGIN      
        1 ctgactaacg agaacggcag ccacagcgac ttcagctacg atgcgctgga ccggttggta
       61 cagcagggcg gctttgacgg gcggacgcaa cgttatcatt atgacctgac cggaaaactc
      121 acgcagagtg aagatgaggg gcttgtcacc ctctggtact acgatgaatc ggaccgtatc
      181 actcaccgca cggtgaacgg cgaaccggca gagcagtggc agtatgatga ccacggctgg
      241 ctgacagaca tcagccacct gagcgaaggc caccgtgtcg ctgtccacta cggctacgac
      301 gataagggcc gcctgaccgg cgaacggcag acggtggaga acccggagac gggggaactg
      361 ctgtggcagc atgagacaac acacgcctac aacgaacagg ggctggcaaa ccgcgtcacg
      421 ccggacagcc tgccgccggt ggagtggctg acctacggca gcggttatct ggcgggcatg
      481 aagcttggcg acacgccgct gctggagtac acgcgcgacc gcatgcaccg ggagacggtg
      541 cgcagctttg gcagcatggc aggcagtaat gccgcatata aactgaccag cacatacacc
      601 cccgcaggcc agttacagag ccagcacctg aacagcctgg tgtatgaccg tgcctacggg
      661 tggaatgaca acggcgacct ggtgcgcatc agcggcccgc gacagacgcg ggaatacggc
      721 tacagcgcca cgggcaggct ggagagcgtg cgtaccctcg caccggacct ggacatccgc
      781 atcccgtatg ccaccgaccc ggcgggaaac cgcctgccgg acccggagct gcacccggac
      841 agtaccctca cggcgtggcc ggataaccgc atcgcagagg atgcgcacta tgtttaccac
      901 tatgatgaat acggcagact gacggagaag acggacctta tcccggcggg tgttatccgg
      961 acggacgacg agcggaccca ccactaccac tacgacagcc agcaccgtct ggtgttctac
     1021 acgcggatac agcatggcga gccactggtc gagagccgct acctctacga cccgctggga
     1081 cggcgaatgg cgaaacgggt atggcggcgg gagcgtgacc tgacggggtg gatgtcgctg
     1141 tcgcgtaaac cggagatgac gtggtacggg tgggacggcg accggctgac gacggtacag
     1201 accgacacca cgcgtatcca gacggtatat cagccgggga gcttcgcgcc gctcatccgc
     1261 atcgaaacag acaacggcga gcgggagaaa gcgcagcgcc gcagcctggc ggagaagctg
     1321 cagcaggaag ggagcgagga cgggcacggc gtggtatttc cggctgaact ggtgcggctg
     1381 ctggacaggc tggaggaaga aatccgggca gaccgcgtga gcagtgaaag ccgggcgtgg
     1441 cttgcgcagt gcgggctgac ggtggagcaa ctggccagac aggtggagcc ggaatacaca
     1501 ccggcgcgaa aagttcattt ttaccactgc gaccaccggg gcctgccgct ggcgctcatc
     1561 agcgaagacg gcaatacggc gtggcgcggg gagtatgatg aatggggcaa ccagcttaat
     1621 gaagagaacc cgtattacct gcaccagcca taccgtctgc cggggcagca gcatgatgag
     1681 gaatcagggc tgtactataa ccggaaccgg tactatgacc cgctacaggg gaggtatatt
     1741 acacaagacc ccattgggct ggcgggggga tggaacctgt ataattaccc actgaatccg
     1801 ataataagga tggatccttt gggtttgtat aatttatatc aattattata tgatgtttgg
     1861 catgatgatt catatggaac atcatcaatt gatattactg gcagtggaga tctaatatca
     1921 ttaggtggtc atgcaggact tggcgttgcg tttgctaaaa agaaaggtga aatgttatct
     1981 gatatttgta tttatgctac agcatgcgga catgcaggaa ttggtggtgg gataaatgcg
     2041 gctatcacat attctgagac caagtcttta cctacatcgg gagtcagcaa ttcagtaggt
     2101 gtaacggttg gcggcggagt tggggggcat tttgcgtata cttatgtagt ggatgttgat
     2161 aatccagaat catcgacaga atctgttggt atcggtgcag gtgttgacgc ttcagttatg
     2221 actctggctt gtagaacgtg gcaagaatgc tgggtcaatt aacaggagga aatatgaaaa
     2281 aatatatatt tttagtttta tttttattgt gtggttgttc tccagtggac atagagccag
     2341 ctagacaaga agttaatatc tttcgtg
//
LOCUS       DAZFYC010000290         2363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFYC010000290 DAZFYC010000000
VERSION     DAZFYC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.296"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(85..444)
                     /locus_tag="ACHKW5_005381"
     CDS             complement(85..444)
                     /locus_tag="ACHKW5_005381"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO7514528.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
     gene            1130..1420
                     /locus_tag="ACHKW5_005382"
     CDS             1130..1420
                     /locus_tag="ACHKW5_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7514529.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            1444..1695
                     /locus_tag="ACHKW5_005383"
     CDS             1444..1695
                     /locus_tag="ACHKW5_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7514530.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1743..>2363)
                     /gene="traT"
                     /locus_tag="ACHKW5_005384"
     CDS             complement(1743..>2363)
                     /gene="traT"
                     /locus_tag="ACHKW5_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO7514531.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          645 a          487 c          514 g          717 t
ORIGIN      
        1 atttttctga ggataagagg tgaatacctc ttatccttaa gtaaatatta accaggtaac
       61 tgtgaataat ttagatgttg ggcgttatga aagtaataaa actccccata taatgatagc
      121 aactaaaatc gtaatcaaca ctgcggctga acccatatct tttgctctgc ctgataattc
      181 gtgatgttct gaaccaattc tgtcaacaac tgcttcaatt gcactgttaa ttatttcaac
      241 aataattacc atcattgccg aaccaatgag taatactcga gtaacagagt caacatccag
      301 ccaaaaggat attatcagtg ctgacagaac cagaatgcac tcctgacgaa aggctgcttc
      361 atagcgccat gccgcacata agcctttcca tgaataaccc gctgctttaa taattctggt
      421 aacaccagtg gtcgtattag ccataaatac ctcttattta attatatgtc tgaatatgca
      481 ttataaaata tcagttaaca ttaacaggat tttgtaataa gccacattta ttctttcggg
      541 aattaaaaaa cataacgaaa ttttgcctca ttctgcattt cattgaaata gtatgtttct
      601 catcgaaagc aagtttgtca atataaattt gtaacagaaa tgactttccg gggataaaca
      661 aaactgttca tggcaatggc tttctctgtc gccaacatgg atacattgtt ccggataaag
      721 cactgatgtc attgactgac gcagactatc aggccatctg atgctttctt ttccttcacc
      781 ggtattgatg atatgcctta tgaaggggga cttattcctg taacccaatc acaggcactg
      841 tgattactgg tttatgattg atgctgattt gagcagatat gcacagcagt tgtggattca
      901 ggggatattt tctgccagat attcatacca gattgatgac ctctttgctc agatgctctt
      961 cgttctgagc cagccaggat tttgtggcca catacattgt tcgccgatgg ggaatcgaga
     1021 caggcgattg gctgtgatgc gattttgccg ctaactgagg atgagtccgc ccctcccggg
     1081 gaattcctgt aagttaagcc gtacctgttg attagcacta aactgggtaa tgaatttatc
     1141 tgccagtatg aatgatgccg gggacggttt tgaactcctg atgtcgggta atatgcagga
     1201 ttttaatatt tgttgtcgtc gggataatca ttatcctgaa cataaactga tacctttatc
     1261 agagttattt gacgttaatt atggactgaa tcttgagctg aataaactgg agaaagattc
     1321 ttcaggaatt aattttgttt caagaacgtc caaaaataat ggcgtctccg ccagggttaa
     1381 attaaaagat ggtattagtc catgccagca gctgtgttaa ctgtagcagc aggaggttct
     1441 gttatggaat cgtttgtaca ggattcacca ttttatagtg gaagagatct ttattggtta
     1501 cgtcctaaag ttgaactgac tctggaagag aaattgtact actgctcttg tataagaaga
     1561 aacagacaca aatacagcta cgggcgtcag gcaaaccgaa cgctgaagaa tttactggta
     1621 ccgtcattag attctgttcc ggcatgggta tatggtgtga cagggaaaat aatatctgaa
     1681 ttgtctgaac gttgagacta agagggccgg aggccaggtg aatggccacc gggatattta
     1741 tatcagagta tattggcgat tgattttgcc agctggtctt ccagatgagg tttagcttct
     1801 tcaaacttca gattaacctg gttggcactg gatacaaccc gggtctggta tttatgctgg
     1861 ttgccggttt ctgtgcttgt ctgaattttg gagcctgatg ttccctgacg caaagctgca
     1921 acattatccg ttctgacgct ggttctggtt cgctcggcaa tctgaacatc tgtaatcatt
     1981 gtgtaattta tatcttccac cattgcatcc gcggccatgc caatcagacc ggtagccagt
     2041 ccaacaccga gagtggtacc tgccgacccg gtattgtacg ctgtgattcc acctccaaga
     2101 gcagctccga ctgctgcacc ttcataaccg cgactcagcc atccctgaga ttcacgcaga
     2161 tccattttgt ctgctttgag aacgttagcc tggatccagt agtaagcttt atccggggaa
     2221 gtcatgatct gataacctct ggctttgact gcatcggcaa tttttccctg tagtccactc
     2281 atgtctttat ccgacgtgtt tttgatctgc aggaaaacgg tgcgttcgct cgcaggttca
     2341 agccagatgg ttgaggtgta ctg
//
LOCUS       DAZFYC010000291         2360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFYC010000291 DAZFYC010000000
VERSION     DAZFYC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.297"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(367..549)
                     /locus_tag="ACHKW5_005385"
     CDS             complement(367..549)
                     /locus_tag="ACHKW5_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7514532.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLSHCARKTR"
     gene            complement(546..956)
                     /locus_tag="ACHKW5_005386"
     CDS             complement(546..956)
                     /locus_tag="ACHKW5_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7514533.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLYQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(1156..1476)
                     /locus_tag="ACHKW5_005387"
     CDS             complement(1156..1476)
                     /locus_tag="ACHKW5_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514534.1"
                     /translation="MDESRKKFEESWLRRGGESSDLIRYPENHHEIGSGNIGGQYVMD
                     DVQGHWQTWQASRSAIEITAPKFIDSREALAKGFTVDYSNGFGDAMDAYEENIRAAGI
                     KVKE"
     gene            complement(1494..1700)
                     /locus_tag="ACHKW5_005388"
     CDS             complement(1494..1700)
                     /locus_tag="ACHKW5_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514535.1"
                     /translation="MVELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLAGVIFG
                     SILLWVIIGVAPVAVAKMAWRFVS"
     gene            complement(1773..2063)
                     /locus_tag="ACHKW5_005389"
     CDS             complement(1773..2063)
                     /locus_tag="ACHKW5_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7514536.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(2060..>2360)
                     /locus_tag="ACHKW5_005390"
     CDS             complement(2060..>2360)
                     /locus_tag="ACHKW5_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7514537.1"
                     /translation="RKRGLYPDAESYPWKSNAHYWLVTNLYQNMRANALTDAELRRKA
                     ADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAKIAELKAKFGLKGASV"
BASE COUNT          592 a          642 c          467 g          659 t
ORIGIN      
        1 actcacaaca aaaaacctca taacacatta acaatcaatt cttttcatct ttataaattc
       61 tcatatcagg cttgcacccg ataaaccgac gaaaactatt taaaacccat cgagtgaagt
      121 aatctctaaa accaaagaaa taccaagtga aaatattcac gataaaatgc ccggtcaaag
      181 cccctcctgt accgcatgca agaacagtaa aaaatcagat gttttcataa atatcagtcc
      241 tcatcgtttt gcctggcatg tcctttacca gcaatcttct gtatgcacta agcctagata
      301 gaatccactc agtgtacact gaagcccgct cgacgctttc ttgttcgtaa cttcgatttt
      361 ggtcaatcac cttgttttcc tcgcacaatg acttagccac cggatatccc acaggtgagc
      421 cgtgtagttg aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga
      481 gcgtttcgtt ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg
      541 tcgtctcatt cgtacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt
      601 gtgtactcga tcagacttgc catacgcttc acgctcatct gcgcgctgct ttcgcgaatg
      661 ttgacgtatt cgccttcaag gccgggcaaa acatcagctt cctgctttgt tgccactgca
      721 tgaccgctta tcaacaaaac cttccattgt tctggtttta accatttatc gcaccattga
      781 acctgacgtg cgatatccgc cagcatcgcg tgaaattttg cgttctggta aaggttgcgc
      841 ttgtagtcag taatgcggat ggtgactggc ttgtctttat cgagtggtgt tgcgaggatg
      901 gcatttattg cagcttgctg ttgttgctta gttcggagga agattgtttg cttcactgaa
      961 tactccttta ttttttatgc ctgtaacccc attcttccag caaccttgcg gcgtaccacc
     1021 caagaaacaa aggaaagaac attacaatga gatattcccc gccacggtca atgttcgaaa
     1081 ttgaccagat tacgatgtaa ccagtgtagg acaggaatat tacaaacccc aaaaagctac
     1141 ttcgtcgact catgctcact ccttcacttt gattccagca gcgcggatgt tttcctcata
     1201 agcatccatt gcatcaccga agccattgga ataatcaaca gtaaaccctt tggctaatgc
     1261 ttctctgctg tcgataaact ttggcgcggt tatttcaata gctgatcgcg atgcctgcca
     1321 cgtttgccag tggccttgaa catcgtccat cacgtattga ccaccaatat taccgctgcc
     1381 aatttcatgg tgattttcag ggtaacggat aaggtctgag gattcgcctc cacgtcgcaa
     1441 ccaactttct tcaaacttct ttcttgattc gtccatcgat acttaccctc agttcaactc
     1501 acaaaacgcc acgccatttt tgctacagcg acaggcgcaa caccgataat cacccacagg
     1561 agaatgctac cgaaaatcac acccgccagg tctttacctt cgcctaccaa ccggacaaaa
     1621 ctgctggcaa ccacaatgaa cgtcgccacc atccacatgg caccgagaat cctcaatgca
     1681 gaaaaaatca actcaaccac gatttactct cccccaaata aaaaggcctg cgattaccag
     1741 caggcctgtt attagctcag tgatgtagat ggtcatcttt taactccata taccgccaat
     1801 acccgtttca tcgcggcact ctggcgacac tccttaaaaa tcaggttcgt gctcatcttt
     1861 ccttcccgtt cttccctggt agcaaaccgg taatacaccg ttcgccagac cttaccttcg
     1921 ataaccagaa gacctgcccg tgccatttta gccgcggcct gatttatgct ggttactgtt
     1981 gcgcctgtta gcgcggcaac gtccggcgca cagaagctat tatgcgtccc caggtaatga
     2041 ataattgcct ctttgcccgt catacacttg ctcctttcag tccgaactta gctttgagtt
     2101 ctgcgatctt cgccagagcc tgtgcacgat ttagaggtct accgcccatg acaggaagtt
     2161 gttttactgg ttcagggatc gcctcaccac ggttaattct cgcagtcata tggacaagct
     2221 catctgcggc cttacggcgt aattccgcat cagtaagcgc attggcccgc atgttctgat
     2281 acaggttggt aaccagccag tagtgcgcgt ttgatttcca cggataagac tccgcatccg
     2341 gatacaggcc tcgcttccgg
//
LOCUS       DAZFYC010000292         2339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFYC010000292 DAZFYC010000000
VERSION     DAZFYC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.298"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..333
                     /locus_tag="ACHKW5_005391"
     CDS             <1..333
                     /locus_tag="ACHKW5_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514538.1"
                     /translation="NMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGG
                     LLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLAL
                     RNAMARIK"
     gene            673..1491
                     /locus_tag="ACHKW5_005392"
     CDS             673..1491
                     /locus_tag="ACHKW5_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7514539.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTTDILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1582..2067
                     /locus_tag="ACHKW5_005393"
     CDS             1582..2067
                     /locus_tag="ACHKW5_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7514540.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            2083..>2339
                     /locus_tag="ACHKW5_005394"
     CDS             2083..>2339
                     /locus_tag="ACHKW5_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO7514541.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEV"
BASE COUNT          557 a          619 c          627 g          536 t
ORIGIN      
        1 aatatggtga ttgtgacgca tctgagtgac ggttctttat gggatcgcca ggcttttccg
       61 gacaccacaa tccttgagat ccgtccccgg aaaaggctga aatatgcagg tgatggtggc
      121 aacagcggcg gtctgctcag ttttacatcg gcacataccg acgcctggcg tcagcagggc
      181 tatgaagaca cgatgctggc gatggagcat atccggaaac cgctggcagc acgtcaggca
      241 ctgacccggt cagaggcggt attgcagaaa agcctggata taacggaaga ggcagatttg
      301 gcactgagaa acgcgatggc ccggattaaa taaagacgcc ccaaagaaac gccaccgcct
      361 gtggtggtgt ttttcctgtc tggtgttatt gaaaatattt tcagatatat attatctggg
      421 tgatggttaa gatgaattta tttaattttg ggggaagata caaaaacaac cagtgaacat
      481 atttccggcg ggttatccgc aatgctgaag gtcagttgca ctggcgatgc cggaatacag
      541 cttaatacgt ggttcgccag tgaagggctt ctcagacaat aaacctcatt catttcaccc
      601 atcaggccgc gtcttctccg ggagacgcgg ctttttcatt tatatcgctt aatcattcat
      661 aaggagcaaa tcatgagatt agccagtcgt tttggttatg cgaaccagat acgtcgtgat
      721 cgtccgctga cacacgaaga actgatgcgc catgtaccga gtatttttgg ggaaaaccga
      781 catacctctc gcagtgaaca ctatgcgtac attcccacta tcaccgtcct ggaaaatctg
      841 cagcaggaag gctttcagcc gttctttgcc tgccagaccc gcgtgcgcga ccagagccgc
      901 cgggaataca caaaacacat gctgcgtctg cggcgggccg gacagataac cggtcagcat
      961 gtccctgaaa ttattctgct caactcccat gacggttcat ccagctacca gatgttaccc
     1021 ggatattttc gtgccatttg taccaatgga ctggtctgcg gtcagtctct gggagaattg
     1081 cgtgttccac accggggaaa tgtggtggac agagtcattg aaggggctta cgaggtggtg
     1141 ggcgtttttg accggataga ggagaagcgt gatgccatgc agtcgctggt cctgccgcca
     1201 ccggcacgcc aggcgctggc acaggcggca ctgacttacc gttatggtga cgaacatcag
     1261 cccgtcacca ccaccgacat tctgacacca cgacgccggg aggattacgg taaggacctg
     1321 tggagtgctt atcagaccat ccaggagaat atgctgaaag gcgggatttc cggtcgcagt
     1381 gcaaaaggaa aacgtatcca tacccgggcc attcacagca ttgataccga cattaagctc
     1441 aatcgcgcat tgtgggtgat ggcagaaacg ctgctggaga gcatgcgctg atgccgtttc
     1501 actgaaagtg cagtcctgac tcacatctgt tcctgccccg gggcttcttc cattcattta
     1561 ctttttataa ggaataacct catgacaacc gtttcgcata attccaccac accttctgtt
     1621 tccgtaacca ctgcatcagg gaataaccca cctcagctgg ttgccactcc cgtccctgat
     1681 gaacagcgca tcagcttctg gccgcagcat tttggcctca ttccacagtg ggtgaccctg
     1741 gagccccgtg tcttcggctg gatggaccgt ctttgcgaag actactgcgg tggtatctgg
     1801 aatctgtaca ccctgaacaa cggcggggca tttatggcac ccgaaccgga tgacgatgat
     1861 gacgaaacat gggtactgtt caatgccatg aacggcaacc gcgctgaaat gagcccggaa
     1921 gccgccggta ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg tacggagtgt
     1981 tatgccatga cggtccacta ttaccggctg cgggattacg ccctgcagca tccggaatgc
     2041 agcgccatta tgcgcatcat tgactgaatg gaggcaggga caatgcagca gctttccttt
     2101 ctgcccggag agatgacgcc cggcgagcgc agtctcattc tgcgggccct gcaaaccctg
     2161 gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc acgggaatgg
     2221 ctgattctga acatggcggg actggagcgg gaagagttcc gggtgctgta tctgaacaac
     2281 cagaatcagc tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg tacggaagt
//
LOCUS       DAZFYC010000293         2265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFYC010000293 DAZFYC010000000
VERSION     DAZFYC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.299"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..404)
                     /locus_tag="ACHKW5_005395"
     CDS             complement(<1..404)
                     /locus_tag="ACHKW5_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7514542.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEV"
     gene            complement(465..1112)
                     /locus_tag="ACHKW5_005396"
     CDS             complement(465..1112)
                     /locus_tag="ACHKW5_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514543.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPVFRQKLNEGWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(1010..1753)
                     /locus_tag="ACHKW5_005397"
     CDS             complement(1010..1753)
                     /locus_tag="ACHKW5_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7514544.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLVAVSTFV"
     gene            complement(1759..>2265)
                     /locus_tag="ACHKW5_005398"
     CDS             complement(1759..>2265)
                     /locus_tag="ACHKW5_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7514545.1"
                     /translation="GFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKV
                     YARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGR
                     IMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGF
                     LSINKLSQ"
BASE COUNT          475 a          759 c          565 g          466 t
ORIGIN      
        1 acttccgacg gattccggtc cccctttgag gtggtttcca ccagtgcctg cacaccgaag
       61 gtaaagcgca gtcggtcgat gtttgccgac gtgatggcgc gggtgatcgg cgtgtcatat
      121 ttcacttctg taccgagcac cgtctcggag ccggaggatt caaatccctc cggcggtgtc
      181 tgctcctgct caccggcccg gaacaccacc gtgacgccgg atatattggt attcccctca
      241 ctgtccagca ccggcgtact gttcagcagc acgcttttta atccatccac cggaccttca
      301 accggccctt cgctgatggc atcgatcaca ctcagcagct gcgtggactt caggttgtcc
      361 ttcgcttcgc gcggggtatg ccccttactg ctgcctttac ccattcctca cgctccataa
      421 acgataaaac cgcccggagg cggtttcaca taaaacattt tgcatcagcg accaatcacc
      481 acaacctgac caccatcccc ttcgtctgcc gtgctgatct cctgagaaac cacacgtgac
      541 cccacgcgca tttcaccgta cagaacaggc agaacattgc cctgggcaac catgttatcc
      601 agtgaggaga aataggtgtt ctgtttgccg ttatctgtac tggctgccgt gggcgtcctg
      661 gctttcggtg ccagcatctg cgccactccg cccaggatca tactggcccc tgccgcatac
      721 atgcccgata cagccgcggc ccccagccag cccacagggt tccaccatgc caccgcaatc
      781 agcgccgccc caagcaccac ctgaaacaca ccgccacttt tagctcccgc cagacgcggc
      841 acgatgtgga tcacggcacc atttgccagc ggctcattaa gacgggcaga taattcattt
      901 tcgcctgcat cacgcccggc aatgcgcacc tgataccagc cctcattcag tttctgacga
      961 aacaccggga gctgtgtggc cagtgcccgg atggcttcag cccccgtttt cacacgaagg
     1021 tcgatacggc gaccaaatcg ttgcaaatcc ccgtaaaggc agatgcgtgc catgcccggt
     1081 gacgccagag ggagtgtgtg cgtcgctgcc atttgtcggt gtacctctct cgtttactca
     1141 gttgttcagg aatatggtgc agcagctcgc cgtcgccaca gtaaatggcg gcatgattcg
     1201 gcaccgatga accaaaacag cacagcagca catcgcccgg ctgggccgct gacaacggca
     1261 cctgatacaa ccccgttgcc tccatattat ccagatagag attctggccg tgacgccacc
     1321 agtcatcccc gcgatgaaaa tccggcatct caattcccgc cagatggtaa gcgtcccgga
     1381 acagcgtgta acagtccgtc accccgtgct caaagcgccg cccggtgaga tgcggcacac
     1441 agcggaattt atgaatcgcc ccccggcaga ccagccacca cggcaaatca ctctgcacct
     1501 gcagccgccg gtcggcctca ctcagccagg gcagaccacc ggggtggctg tggaccagcg
     1561 ccacaatctc tccctgcatt tctgcactca gccagtcctc cggcgacatc cggaaatacg
     1621 cctccggctc accagagata ttcacgcagg gaaaatatct ttccccctcc ggtgttctca
     1681 ccacgaagcc gcacgactcc gctggcgcac atcgccgggc gtgcgccaga atcgctgatt
     1741 ctgtctctgt catgggattc actgcgaaag tttgttaatg gaaaggaagc cgccaaagtt
     1801 gccgacgtta ttgcgaaact tacagccgct caggcatttg ctgcatttat ccttcgtgat
     1861 atcggacgtc ggctgatcat attcatccgc gaccgccgga ccgtgataac cgcactcgtc
     1921 accgcgatag gtccaggtgc aggtgttggc cagcatgatg cgccccggaa aaacggcacc
     1981 gtccgtttcc gtcggcgtgg acagtacaaa agaggcactc accgcgctca gttcgctgca
     2041 ctgctcgatg cgccagcggc tgatcacctc ctgctccgga tcggcgtaac tgtttccgtt
     2101 gacgaagttc accgcatcca gaaaacgggc gtaaacctta cgccggacca ccgttccgcc
     2161 gaccagactc tgcatatctt ccgccatccc ggtgaccata ccgtacaggt tagaaaccgt
     2221 cagcgtgggg cgcgtactgg tgcctttgcc attcagttca aaacc
//
LOCUS       DAZFYC010000294         2258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFYC010000294 DAZFYC010000000
VERSION     DAZFYC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.300"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(28..525)
                     /locus_tag="ACHKW5_005399"
     CDS             complement(28..525)
                     /locus_tag="ACHKW5_005399"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO7514546.1"
                     /translation="MKVSFVSQDDFENLIQEAFASVRLRSHLLLHESPNDAVQRIMIG
                     LVKGTYIPPHFHEFQHQWEHFHVFQGEVELMLFDSNGCLNKKVILGGQSKNIIAQISP
                     LTPHTLVCRSPTAVIMEIKEGPFDEKCAKVIPSWSYSEDYSILSRDRIIAMMGQLSIG
                     DRFSL"
     gene            complement(544..1737)
                     /locus_tag="ACHKW5_005400"
     CDS             complement(544..1737)
                     /locus_tag="ACHKW5_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7514547.1"
                     /translation="MRIALICDDYLPDSTRVSAKMMHELACELLEKGHEPIVICPCNK
                     IQTLEILNLDGVVVYKFPNGAIKNVSKISRAINESMLSFNAWRFIGKYIRETKIDGVV
                     YYSPSIFFGKLANKIKENWHCKSYLILRDSFPQWLVDQGIIKAGGLAERYFRYFEQIN
                     YDAADYIGLMSDRNKDIFINKYQNKYKVQTLFNWADFKGIDNIPSATLRSKLALQNKV
                     IFFYGGNIGHAQDMMNLMRLVRSASYRDDVHFLLIGQGDEVSLVKQFIIDNYLKNCTY
                     LPSITQSEFKSVLKIVDVGLFSLAKNHTVHNFPGKLLGYMANKLPILGSVNASNDVME
                     IINGAKAGFVFVNGNDEALLNAAINLADDTQLRKNLGCNAYSLLQEKFSVEMAAEKIL
                     SSLFS"
     gene            complement(1737..>2258)
                     /locus_tag="ACHKW5_005401"
     CDS             complement(1737..>2258)
                     /locus_tag="ACHKW5_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFO7514548.1"
                     /translation="HREENVDSPEKLRSLIETLNIVSEKYKLPVIVSTHPRTRNRIDA
                     LGITVSNNIIFSKPFGFLDYIKLQQNARVVLSDSGTITEESSVLNFPALNLREVHERP
                     EGFEEAVVMFVGLDRNRIIQGIDILQGQKRGDNDRDLHMVTDYQADNVSIKILRIIMS
                     YTNFINQKVWKKF"
BASE COUNT          741 a          451 c          333 g          733 t
ORIGIN      
        1 agattacttc gctaattgct gtgaagttta gagactaaat ctatctccaa ttgataattg
       61 gcccatcatt gcgatgatcc tatctctgga taaaatggaa taatcttcac tatatgacca
      121 tgaggggatg actttagcgc atttttcatc aaaaggacct tctttaatct ccatgataac
      181 tgctgtagga gatctacaaa ctagtgtatg tggagtgagt ggtgaaattt gtgcaataat
      241 attcttactc tgcccaccaa gaataacctt tttatttaaa cagccgttac tatcaaatag
      301 cattaattca acctcacctt gaaacacatg gaaatgttcc cattgatgct gaaattcatg
      361 aaaatgaggc gggatatacg ttcctttgac aagtcctatc attattctct ggacagcatc
      421 attagggctt tcatggagga gtaagtgaga tctcaagcga acggatgcaa atgcttcctg
      481 aattaaattc tcgaaatcat cctgacttac aaaagacact ttcataaaac caccatatta
      541 ctgttaggaa aataagctgc ttaatatctt ttcagcagcc atttccacag agaatttttc
      601 ttgtaataaa gagtaagcat tacatcctag gttttttctt agctgagtat catccgcaag
      661 atttattgca gcattaagta aagcctcgtc atttccgtta acaaaaacaa acccggcctt
      721 tgccccattg ataatctcca taacatcatt gcttgcatta acactaccta atatagggag
      781 tttatttgcc atatacccta acaatttacc agggaagtta tgaacagtat gatttttagc
      841 aaggctaaac aaaccaacat caacaatctt taatacagac ttaaactctg attgtgttat
      901 ggatggtaaa tacgtacagt tttttaaata attatctata ataaactgct ttacaaggga
      961 aacttcatca ccttgtccaa tcaataaaaa atgcacatca tcacgataag atgctgatct
     1021 aaccaacctc attaaattca tcatatcctg agcatgacca atattgcctc cataaaaaaa
     1081 aattactttg ttttgaagtg ctaattttga tcgtagagtc gcactgggaa tattatctat
     1141 acctttaaag tcagcccaat tgaataaagt ttgcaccttg tatttattct gatatttatt
     1201 aatgaaaata tctttgtttc tatccgacat taaaccaata taatctgcag catcataatt
     1261 gatctgctcg aagtacctaa aatatcgttc cgcaagccct cccgccttaa ttatcccctg
     1321 atctaccagc cactgaggaa aagaatccct taagattaaa tatgatttac aatgccaatt
     1381 ttctttgatt ttatttgcta attttccaaa aaaaatagat ggtgagtagt acacaacacc
     1441 atcaatttta gtttctcgaa tatattttcc aataaatcgc catgcgttga atgatagcat
     1501 ggattcgttt attgctcttg atattttaga tacattcttt atagcaccat tagggaactt
     1561 ataaacgacg acaccatcca aattcaaaat ttcgagcgtc tgtattttat tgcaaggaca
     1621 aataacgatt ggctcatgtc ctttttctaa aagttcacaa gccaattcat gcatcatctt
     1681 agcactaaca cgagtactat caggcaaata gtcatcacat attaacgcaa tacgcattag
     1741 aattttttcc aaaccttttg attaataaag tttgtatagc tcataataat tctaagaatt
     1801 ttaattgaaa cattgtctgc ctgataatct gtaaccatat gaaggtcacg atcattatca
     1861 ccacgttttt gaccctgcaa aatatcgatt ccctgaataa ttctgtttct atctagcccg
     1921 acaaacataa caacagcttc ttcaaatcct tctggtctct catgtacttc tcgcaggttt
     1981 aaggctggaa aattcaaaac tgatgattct tcagttatag ttccactatc agagagcacg
     2041 acacgagcat tttgctgcag ttttatataa tctaaaaatc cgaatggctt agaaaaaatg
     2101 atattattac ttaccgtgat acctaaagca tcaattctat tacgagtacg aggatgcgtt
     2161 gatactatta ctggcagttt atatttctca gatactatat ttaacgtctc aataagagag
     2221 cgcaacttct caggcgaatc aacattctct tctcgatg
//
LOCUS       DAZFYC010000295         2252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFYC010000295 DAZFYC010000000
VERSION     DAZFYC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.301"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1374)
                     /locus_tag="ACHKW5_005402"
     CDS             complement(<1..1374)
                     /locus_tag="ACHKW5_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7514549.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEYYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMS"
     gene            complement(1349..1894)
                     /locus_tag="ACHKW5_005403"
     CDS             complement(1349..1894)
                     /locus_tag="ACHKW5_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7514550.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            2008..>2252
                     /locus_tag="ACHKW5_005404"
     CDS             2008..>2252
                     /locus_tag="ACHKW5_005404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514551.1"
                     /translation="MFEKYRHDFPRKIFINSENPRGRRPVTGRIAGKDPQNDNNYHLH
                     VTTCIYAIKPRQIINYDAGIVLIDLHQINVKATSDNT"
BASE COUNT          548 a          636 c          522 g          546 t
ORIGIN      
        1 cgacatttct gcaccattcc ggcgggtata ggttttattg atggcctcat ccacacgcag
       61 cagcgtctgt tcatcgtcgt ggcggcccat aataatctgc cggtcaatca gccagctttc
      121 ctcacccggc ccccatcccc atacgcgcat ttcgtagcgg tccagctggg agtcgatacc
      181 ggcggtcagg taagccacac gatcaggaac gggcgctgaa taatactctt tccgctctgc
      241 catcacttca gcatccggac gttcgccgat tttcgcttcc cacgtctcac cgagcgtggt
      301 gttcacgaag gttttacgtt ttcccgtatc ccctttcgtc ttcatccagt ctttgacaat
      361 ctgcacccag gtggtgaacg ggctgtacgc cgtccagatg tgaaaggtca cactgtcagg
      421 cggttcaatc tcttcaccgg atgacgaaaa ccagagaatg ccatcacggg tccagatccc
      481 ggtcttttcg cagatataac gggcatcagt aaagtccagc tcctgctggc ggatgacgca
      541 ggcattatgc tcgcagagat aaaacacgct ggaggggtca tccggcgtcc atttgaggcc
      601 aaacggcgtc tctttgtcgc caaatttaag atactgctcc tccccgcagt gcgggcaggc
      661 aacatgaaaa cgcataaaat gcggggattc actggctgca cgctcaatct ggcaggtgcc
      721 tctcactttg ggcgtggagc cacggatgga ctttggccag accgagcctt caatacgctt
      781 gtcacccagg aacgtcggag agccttcctg ttcaatatcc tcatcaaagg cagcaagttc
      841 atcataaccc gccacatcaa ccgacttttc acggtagttt tttgccgctt taccgcccag
      901 gcaccagaag ccacgaccat tggtgaaacg cttcatagtg agcgtgttat cccggtgctt
      961 tttgccatac cacggggcca gagacagcag cgacggaata tcgcggatgg taggctcgac
     1021 gtgggttttc ataaaattct cggcatcacc atccgtcggc aaccagataa gggtgttacg
     1081 ctgcttatgc tctatgaagt aagcataaac acccagcagc atttttgaat aaccaacacg
     1141 ggcagacttc accacattca cctcgcggat gtagtcgctg cccatcgcat tcatgatagc
     1201 ccgctgaaag ggcagtgttt cccagcgccc ttcctggtat gcggattctt tcgggagata
     1261 gtaactggca tccgcccatt caacggcagt ctgtggctcc ggcctgaaca gggctcgcag
     1321 cccggcgcgt acatcacgcc gcagaatatc aatctgactg ttcgatatat tcactcagca
     1381 accccggtat cagttcatcc agcgcggctg ctttgttcat ggctttgatg atatcccgtt
     1441 tcaggaaatc aacatgtcgg ttttccagtt ccggaaaacg ccgctgtact gagagaggga
     1501 tcccgtcaag aatactggca atttcacctg cgatccgtga cagcacgaaa gtacagaatg
     1561 cggtttccac cacttctgcg gagtctctgg cattcttcag ctcctgggcg tcagcctgcg
     1621 cacgcgtaag tcgatggcgt tcgtactcaa tagtcccagg ctggagatct gcctcgctgg
     1681 cagccctgta atcctcaacc tctttacgga gtttttcatt ttcgatatca gcttccctct
     1741 gcgcatacca ctgaattgcc gtggcggtat caaatacaga ttcaacgccc ttaccacctc
     1801 cggagacgca agggagcccc tgagactgcc agcgttcaat cgttcgcgga tccacgttga
     1861 aaatttcggc aagtttcttt ttattaacct tcatgaaaca gtctcacaac aaatacagga
     1921 tccgacatga aagtgcccga aaatgacttt ttcaggcgtt ttcatgtcgg acctttacgg
     1981 attcgatatt agaaaaaaca aatagttatg ttcgagaagt accgacatga ttttccccgg
     2041 aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtaaccgg tcggatcgcc
     2101 ggaaaggacc cgcaaaatga taataattat catctacatg tcacaacgtg catctacgcc
     2161 atcaaaccac gtcaaataat caattatgac gcaggtatcg tattaattga tctgcatcaa
     2221 attaacgtaa aagcaacttc agataataca aa
//
LOCUS       DAZFYC010000296         2212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFYC010000296 DAZFYC010000000
VERSION     DAZFYC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.302"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..106)
                     /locus_tag="ACHKW5_005405"
     CDS             complement(<1..106)
                     /locus_tag="ACHKW5_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514552.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVF"
     gene            complement(90..>2212)
                     /locus_tag="ACHKW5_005406"
     CDS             complement(90..>2212)
                     /locus_tag="ACHKW5_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7514553.1"
                     /translation="HYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSHTSEGHRVSVH
                     YGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYGS
                     GYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLNS
                     LVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGN
                     RLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHH
                     YHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEE
                     TWYGWDGDRLTTVQTDTTRIQTVYQPGSFTPLIRVETENGEREKAQRRSLAEKLQQEG
                     SEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPE
                     RKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDK
                     ESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSG
                     GLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFG
                     STIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQL
                     FGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          404 a          698 c          559 g          551 t
ORIGIN      
        1 aaaatacgat gtcagaattt gaattgttat caaacccaag gtaaacaaca aataaacaaa
       61 gggtaattaa aaaaatgaca agtttgcctc taataatttg aagcatctga actcccttgc
      121 tttgctttta ttggattatc tgcttttata ccatctaacc cgaagagttg cccagtgtta
      181 tatttttcaa tacatgcatc gctacatttg gcagaaaaat cgccacaagc aataccgaca
      241 gtgttatttt taagatccat gattctttct ttagctggct gaccttgtct atcccctgcc
      301 gcctcatgat ttttacctat cacgtcagct attgttgatc caaatgtctt agtcattcga
      361 cacatcaagt aacaatgtct cattgcatcg gcggccccat tatgagctcc gccaacaata
      421 gcatccatat cataaccgaa ctggtaagca ctgttagtaa tgctgtttgc agcaatcaat
      481 ccaggtacac cagatacggc ataaattagt ccacctgatg tcatattctc aatcgcatct
      541 aaccccattg ggtctatcac ttgtatggga ttcaacggat actgatacat attccatcca
      601 cctctcaacc caatcgggtc cggggtgata taccgcccct gcaacggatc gtagtaccgg
      661 tgacggttat agtacagccc cgactcctta tcatactgct gccccggcag ccggtacggc
      721 tggtgcaggt gatgcgggtt ctcctcatta agctggttgc cccattcatc atattccgcg
      781 ctccacgccg tattgccgtc ttcactgata agcgccagcg gcaggccccg gtggtcacag
      841 tgataaagat gtatttttcg ctcaggcaaa taaaccggct ctatctgtgc cgccagccgc
      901 tccaccgtca gcccgcactg cgcaagccac tggcggcttt cactgctcac gcagtttgcc
      961 cggatttccc cctccagcct gtccagcagc cccaccagtt cagccgggaa caccacaccg
     1021 tgaccgtctt cgctcccttc ctgctgtaac ttctccgcca ggctgcggcg ctgcgctttc
     1081 tcccgctcgc cgttctctgt ctcgacccgg atgagcggcg tgaagctccc cggctggtat
     1141 accgtctgga tacgcgtggt gtcggtctgt accgtcgtca gcctgtcgcc gtcccagccg
     1201 taccatgtct cctccggttt acgcgacagc gacatccacc ccgtcaggtc acgctcccgc
     1261 cgccataccc gtttcgccat tcgccgtccc agcgggtcgt agaggtagcg gctctcgacc
     1321 agtggctcgc catgctgtat ccgcgtgtgg aacaccaggc ggtgctggct gtcgtagtgg
     1381 tagtggtggg tccgctcgtc gtccgtccgt atcacacccg tcgggatacg gtccgtcttc
     1441 tccgtcagtc tgccgtattc atcatagtgg taaacatagt gcgcatcctc tgcgatgcgg
     1501 ttatccggcc acgccgtgag cgtgctgtcc gggtgtagct ccgggtccgg caggcggttt
     1561 cccgccgggt cggtggcata cgggatgcgg atgtccaggt ccggtgcgag ggtgcgcacg
     1621 ctctccagcc tgcccgtggc gctgtagccg tattcccgcg tctgtcgcgg accgctgatg
     1681 cgcaccaggt cgccgttgtc attccacccg tagtcacggt catataccag gctgttcagg
     1741 tgctggctct gtaactggcc tgcgggggtg tatgtgctgg tcagttcgta tgcggcatta
     1801 ctgcctgccc tgctgccgaa gctgcgcacc gtctcacggt gcagcctgtc ccgcgtatac
     1861 tcgaccagcg gcgtcccgcc cagcttcatg cccgccaggt aaccgctgcc gtaggtcagc
     1921 cactccaccg gcggcaggct gtccggcgtg acgcggtttg ccagcccctg ttcgttgtag
     1981 gcgtgtttcg tctcatgctg ccacagcagt tcccccgtct ccgggttctc caccgtctgc
     2041 cgttcgcccg tcaggcggcc cttatcgtcg tagccgtagt ggaccgacac gcggtggcct
     2101 tcactggtat ggctgagggt ggttaaccac ccgtgctcat catactgcca ctgctctgcc
     2161 gggtcgccgt tcaccgtccg gtgcgtgatg cggtccgacg catcgtagtg cc
//
LOCUS       DAZFYC010000297         2206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFYC010000297 DAZFYC010000000
VERSION     DAZFYC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.303"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            100..1083
                     /locus_tag="ACHKW5_005407"
     CDS             100..1083
                     /locus_tag="ACHKW5_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7514554.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKAGVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            1213..2206
                     /locus_tag="ACHKW5_005408"
                     /pseudo
     CDS             1213..2206
                     /locus_tag="ACHKW5_005408"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
BASE COUNT          538 a          484 c          611 g          573 t
ORIGIN      
        1 gctgtttatt ctgcataaaa atattcgaaa attcttattc tctccagcgc gctaatttgg
       61 gcgaacactt cctgactacc ctgcaatgag gctgaaatga tgaataaacg ttttgttatc
      121 aatatggtgt cttcgctgtt attaggcgct gcgctaattt ctgcgccgct ccaggcagcg
      181 gaaaaagtcg tggtgaatat ttctaaagtc gatggtatgc cgtggtttaa tcgcatgggc
      241 gaaggtgtgg ttgaggcggg taaagccttt ggtgttaatg cctcacaggt ggggccttca
      301 agtactgatg ctccgcaaca agttaaaatt attgaggatt taatcgcccg taaggttaat
      361 gcaattacta tcgtgccaaa cgatgccaac gtgctggaac cggtattcaa aaaagcgcga
      421 gatgccggaa ttgtggttct gaccaacgaa tcccccggtc agccaagtgc taactgggat
      481 attgaaatca ttgataacga aaaatttgcc gctgaatatg ttgagcatat ggcgaaacgc
      541 atggggggca agggcggcta tgtgatttac gtgggtagtt tgaccgtgcc gcagcataac
      601 ttatgggctg acttgctggt gaaataccag aaagagcatt acccggatat gcatgaagtt
      661 acccgccgca tgccggttgc cgagagtgtg gatgactcac gtcgtaccac actggacctg
      721 atgaaaacct atcctgactt gaaagcggtt gtctcctttg gttcaaacgg tccgattggc
      781 gcgggacgtg cggtgaaaga gaaacgggcg aaaaataaag tcgcagtcta tggcatgatg
      841 atcccttcac aggcggcatc actgattaaa agtggtgata tcaccgaagg gattacttac
      901 gatccggcct ctgcaggtta cgcactcgca gccgtcgcca gcacgttgct gaagggtgaa
      961 gagatcaaac ctggattaga aatgcagaat ctgggtaagg ccggcgtaga tatggataag
     1021 cgaattatcc gtttccataa ggtattactg gtcaacaaag acaatatcga ctcactttat
     1081 taatccattt cttcggggcg aaagccccga ttcttattgc caggggatgt tatggaaact
     1141 ttcctttccc ttcgtcatat caacaaaacg tttcatgcca cgcgagcgct gcgcgatgtt
     1201 tctctcgact ttatgtcggg tgaagtgcac tgccttgccg ggcaaaacgg ctgtggtaaa
     1261 tcgacgttga ttaaaattat gtccggtgtt tatcgcccgg atgagggagc ggaaattacg
     1321 cttggtggga aaaactgatc aaagctgaca cccgccgctt cggtggcgca ggggattcag
     1381 gtgatttatc aggacctctc tttatttcct aacctgagtg tctgggagaa tatcgccgtg
     1441 aatcactatc accacggtct gtttgttaac cgccgtcgtc tgcgtgaggt ggcgcaggcg
     1501 gcaatgacca gtattaacgt cacattgcca ctggatacac tcgtttctga gctttcaatt
     1561 gcccgctgtc agctggtggc tatttgccgt gcactggcgc aggacgcacg gttgatcgtg
     1621 atggatgaac caaccgcttc gctgacccat caggaagtgc aagggttatt acaggtggtg
     1681 catcaattgc gtgaacgcgg gatctgcgtg gtcttcgtca gtcatcgtct ggaagaagtg
     1741 atggaagttt ccgaccgtat ttcagtgctg aaagatggtg agctggtcgg gacttttccg
     1801 gctgcagaga tgaccacaaa acaactcggt ttcctgatga ccggccagga gtttgaatat
     1861 caggtgcgag agttgtggca gggaaaatcc agcaccccgg tgctggaggt gcgtaattta
     1921 agtcgtcatg gggaatatct aaatattaac ttgcgggtgg aagcgggcga agtggtatcg
     1981 attgttggcc tgcttggtgc ggggcgcacg gaattatgtc tgagtctgtt tggtatgacc
     2041 cgaccggatg ctggcgagat cctcatcaac ggccagccgg ttacactgca tagtaaccag
     2101 gacgctatcc gccacggtat tggttatgtt tcggaagatc gcatgtcgcg cggtttggtg
     2161 atggcgcaat ctattgaaga caacatcatt agcactgttt ttcaca
//
LOCUS       DAZFYC010000298         2187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFYC010000298 DAZFYC010000000
VERSION     DAZFYC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.304"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..1590)
                     /gene="cadC"
                     /locus_tag="ACHKW5_005409"
     CDS             complement(52..1590)
                     /gene="cadC"
                     /locus_tag="ACHKW5_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO7514555.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1850..1964)
                     /locus_tag="ACHKW5_005410"
                     /pseudo
     CDS             complement(1850..1964)
                     /locus_tag="ACHKW5_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          636 a          442 c          464 g          645 t
ORIGIN      
        1 acaaaaactc aacaacaaat attcccgagc ataaatcaac ccgggagtta cttattctga
       61 agcgagaaat ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt
      121 ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc
      181 agccgcttcc cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag
      241 ccaggacatt tcaagatcaa tgccagtatt tatcgcctgg taagactcat ctgttttacc
      301 ttttaccaga gcactgaccg cttttatttg atatataatg gacaggttat tcaattccgg
      361 cagtgtaaca atgttgtcta tttctgtgtt cagtgctgct aattgttttt catctaaagg
      421 atgttgagaa tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc
      481 tggggatgat tgaacaatct cacctaataa ttcactggca cgattcaatg atttatcatc
      541 gccatgcagt aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgagg
      601 caatatttgc tggagcgtct cctgcattcg ttgtggccac ggttggttta acgcttttga
      661 taaactctcc agtaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg
      721 tttatccagc atggttgaac catctgcatt gtctaccaat tttatcgaca taaagcattg
      781 ttgagcacgg tattggcgct gattaacaaa cgcaatagat aatgttttac cggaactgct
      841 cggttcatca atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag
      901 tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag agctgatacg gggaactcca
      961 gctgttacaa cttttattta ccatattaat gtcaatatcg cgtggattga gcaaaatacg
     1021 cgatttgctc ataggaagac gtgtatcaag agttgaaaac gctaccagtg ctacacagat
     1081 acctaacgac aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac
     1141 tggggattgt tcaggtggcg ttgcggtgtt ttgaatgtta agactgtggg agggagaatc
     1201 tgtggcagga accgcctctg gtataggggg aggcgaagat agcattattt cctctccctc
     1261 ttcttcgctg taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc
     1321 gatatagaca ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact
     1381 ctgcgtcaca acatgattgg tgacaatact tctcttccag acattatcga taagttcatc
     1441 cctgctaagt acttcgccac tgtgttgagc aaagaaaacc agaagatcga ttaatctcgg
     1501 ctcaagggta agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca
     1561 ttcgccaacg cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatgatg
     1621 caatgataat tgccacaacc tatttttacc atctatagat gggtttattt acatattatt
     1681 ggtgaatgca agacgttatt tttaccagcc ataaacttct tgattacata gtattacgaa
     1741 aggattttac tgagaaccag aagtaatatt ccttaccatc aaaattcatc atctttgcca
     1801 aagaaaaatg ttcagaaaat aatccatgga aaattgtccg gagcgcttac tattttaatg
     1861 gattgttagt ctttgcatga gcaagcggac tgatacattt ctctttgttc tcattcagaa
     1921 aatctcatca gtcggcgttc agaccggtgt tgtgctttat ggatgcgtta cgctcctgat
     1981 gacgtcattt gacgttcaac aacatcacgg ggccgcacga catttcacgt cagttagtgc
     2041 tatcgctcag gaacaaattt tcccgaattg ggatatgccc gcaaattgct ggtgatgtgg
     2101 ggagaatctg gttgagttcg ggagaattga tttggaggca gaacgcttaa attgtggcgt
     2161 ccagaaacga aaaacggacc tccgtgg
//
LOCUS       DAZFYC010000299         2161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFYC010000299 DAZFYC010000000
VERSION     DAZFYC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.305"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..107)
                     /locus_tag="ACHKW5_005411"
     CDS             complement(<1..107)
                     /locus_tag="ACHKW5_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514556.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHAL"
     gene            complement(104..667)
                     /locus_tag="ACHKW5_005412"
     CDS             complement(104..667)
                     /locus_tag="ACHKW5_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7514557.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(957..1322)
                     /locus_tag="ACHKW5_005413"
     CDS             complement(957..1322)
                     /locus_tag="ACHKW5_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7514558.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELEHRIALQFGGGNEETNLWTLCTECHRQKSAREAASGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            1364..1549
                     /locus_tag="ACHKW5_005414"
     CDS             1364..1549
                     /locus_tag="ACHKW5_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7514559.1"
                     /translation="MSSKNRTRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(1679..1819)
                     /locus_tag="ACHKW5_005415"
     CDS             complement(1679..1819)
                     /locus_tag="ACHKW5_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514560.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1961..>2161)
                     /locus_tag="ACHKW5_005416"
     CDS             complement(1961..>2161)
                     /locus_tag="ACHKW5_005416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514561.1"
                     /translation="HARYCRRIPALTETAARPGESSARVCGDNQKRYTPGFTALTERG
                     VVPSWSLVRCDGGRSRMFITIN"
BASE COUNT          560 a          605 c          478 g          518 t
ORIGIN      
        1 agtgcatgtg cagcctggtc cggataaatc ggagccggaa taatcgacag cccctttttc
       61 aggcgctctg cccagtccgg gcaggccgtg ctccacacag gtatcatccg ttgccctcat
      121 tatcattatt caccaccagg cggggtggtg gtggcaccgc aaaacggtta gccgcttttt
      181 tcgccgcgtc accttttgcc gattttttac cggcatcccc ttttttatgg tgcgtgaact
      241 gcgccagctt ataagccgca tccagcgcca gcctggggtc ggtattaatg ttctccacca
      301 gaagacgccc catcgctttc accggatcgg gaagaccatc ctccatatat tcaataccag
      361 gagacatcac cgcggacggt ggcatctccg gattgttttc gtccggctgt ggtattgcag
      421 ccgcctcacg gcgacggggt ttatcctcct gctctgattt tttctgccgg taaacaggaa
      481 cctcatccac ctccaccgtc tcgcattgtt tacgggctat aaacgcaagc acctccggat
      541 cttttgccag ctgcgagcct ttaaccctgg cggtcttcgc cgaataacca gcggcaatgg
      601 ctgacgctgt tttgtttttc ccggacatga gcgccagcgc aaattttcgt ttttgcgttg
      661 tcagcacagc ctcctcccgg gtccagaacg cactcagccg ggtatggttc agcccatttt
      721 tcccggcgtc tcatgccgca aatgttaact gctgcctggt taacatttgc tgaaaaagcc
      781 agttaacatt ttttccgcac aacaaactga ataataaaga taaaaaccgc aaaaatgccc
      841 ggacagccag ttaacatgtt aactgccctg aaacgggaat tttttctctg cgtgagaggg
      901 ggggcggtgt ccaaagcgat ctttttttac gccggatgat acccccccgg gtcgggttac
      961 agtccgatga tgtcgtccgc tctgccacta cctccggaca cctccggcag cgtcgggtct
     1021 ggcataccac tcgccgcttc acgagcagac ttttgtcgat ggcattcggt acagagcgtc
     1081 cagagattcg tctcctcatt accaccaccg aactgaagtg caatgcggtg ctcaagttca
     1141 ctgtcacaaa ggtcaaccac acgcccacag agacagcact gtccggcatc cctcagccag
     1201 atacgacgct tgagggaaac ccgggcacta ccactgaccc gacgctgttc acccttcagg
     1261 acattcaccc gccgggtgtt cagagttttg attctgcccg gtaacgtacg aagcacagcc
     1321 atgtaaaatc ctctccatat agcttgtcac cagaggaaag aaaatgtcat cgaaaaaccg
     1381 gacccgcaga accacaatcc gcaatatccg tttccccaat cacatgattg aacagatcaa
     1441 tatcgccctt gaacataaag gttccggtaa cttttcagcg tgggttattg aagcctgcag
     1501 aagaaggctg tcaacagagc gttcgggtat gaattacata attaagtaac atggtgttca
     1561 cagaacacac agttaccgga cacatcagtt ttccattcgt cccccggcag tacaggcttc
     1621 ccgtctgacg ggatagcctg aaaaaacaca gaaaattatt tgttataatt aatataactt
     1681 actcaaaaaa aagcgacgag aaaatcagca tcaacgagca ataagcgcca atacgtgata
     1741 acaaatggca gccatattta tctgcagtat aagcaatgga caggataacc acaccagaaa
     1801 ccgtcagcat aaaatccatt tgaacttccc cggacaaaat cgactcatct aaagatttac
     1861 agctcttttt attatcaata tgttaaaagt aaaataaaca gatgttcaat aatacgaata
     1921 caaaaacgtg ctgaaaatca atgaatccat ttctgtgtta tcaattaata gtgataaaca
     1981 tccggcttct tccaccatcg caccggacca gcgaccatga ggggacaacg ccgcgctccg
     2041 ttaacgcggt aaaccccggt gtgtatcgtt tttgattatc cccgcacact cgcgcagagg
     2101 attctcccgg tcgggctgcg gtctctgtta atgcaggaat acggcgacaa taccgcgcat
     2161 g
//
LOCUS       DAZFYC010000300         2146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFYC010000300 DAZFYC010000000
VERSION     DAZFYC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.306"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(286..569)
                     /locus_tag="ACHKW5_005417"
                     /pseudo
     CDS             complement(286..569)
                     /locus_tag="ACHKW5_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(715..2091)
                     /gene="nleA"
                     /locus_tag="ACHKW5_005418"
     CDS             complement(715..2091)
                     /gene="nleA"
                     /locus_tag="ACHKW5_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO7514562.1"
                     /translation="MNIQPIVQSGITTQNNQHNQAEQAPPAQIPQSELPNGCEAGFVV
                     HIPDDIQQHAPECGERTALLSLIQDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     TQMALEIRSTVPGISGKYGVQMNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASAATSAATSAASTAVSAITSAATSATTSTVTTEPKDLIPW
                     FGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGT
                     SPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGAAGGFAVSKIPGVDSMLAPH
                     IGAMVGQAAGHAIQCNTPGLKPDTILWWAGTTLGLADLNKAETGEARFTDYPRIWWHA
                     REGAIFPNKEDIARATGADIRAMEEGVPVDHRHPHPEDVVIDIESSPHNNPSNYVDTV
                     DIIRETRV"
BASE COUNT          574 a          456 c          452 g          664 t
ORIGIN      
        1 tcgatttgta aatcgcttac aactggtttg attctgttcg ccatatcact tgacggttta
       61 ttatttgcgt tttcatcatt tggcccagta gtgaggaagc cagaaataca tctttgactg
      121 ggttcgggct ttgctgcaaa catatctgct attccttatt ttataaatgc atttcccaat
      181 tctagtttgc atacatttct tgtggctaaa ataaacactt gtcatccatt ttgcaaccac
      241 atgttaacaa aataagttgc tataccgata aagggatact tttgcttagt taaccacacc
      301 attttcttcg gacacaggaa attccctgaa ggtggacgag cgattctgat gaatgcagca
      361 gcaggcattg actcccggtt ctccgtccaa ggccacaaat cgaggatttt tctggttagt
      421 gccctgcatg cttctatggt tcggagagat ttgctcagtc agggttacgg ggatgatgcc
      481 accagcatca tctgcttcag cctggtaaaa agagacgcgg aaaatgcaca acaggcacca
      541 cacgtcatgc atggattagg attgttcata aattcactac gcaagtatta gtaacgttaa
      601 gggataacag gtgtctgaaa atatagagtc aaatccaaga aagaatatat tgttccttat
      661 taataatagc tgtcatccat ttcagctatt attttaaaat aaacaagtta aagcttagac
      721 tcttgtttct cggattatat caaccgtatc aacataattt gatggattat tatgtggaga
      781 gctttcgata tcgatgacca catcttcagg atgtggatgt cgatgatcaa cagggacacc
      841 ttcttccatc gcacgtatat cagcccctgt tgcacgggca atatcttctt tattggggaa
      901 gatggctcct tctcttgcat gccaccatat acgaggatag tcagtgaatc tggcttctcc
      961 ggtctcggct ttgtttaaat ctgctagccc cagagtcgta ccagcccacc ataaaatagt
     1021 gtctggcttt aatccggggg tattacactg tatggcatgc cctgctgctt gcccaaccat
     1081 tgcgccgata tgaggggcta acattgagtc tactccgggg attttggata ctgcaaaccc
     1141 tcctgcggcg ccaagcgcat ttgcggcagc attggtaaga acggcatatt tagtattagg
     1201 gttatctaaa tcccatttga aatgtgcgcc aaactccagc cctagagttg cctgtagtgg
     1261 agatgtacca tatttaaaat ctgcttctcc cggtctgata acccactcct ccgtgtaatt
     1321 tgcttttggc ccatcaggag taagaggcgt ttctccaaca agcttccatc cattttcagt
     1381 tttgactata ggaagttcca caccatggtt acgaactact tgagctgtta atccaaacca
     1441 tggtattagg tcttttggct cggtggttac cgtcgaagta gtggctgatg ttgcagccga
     1501 tgtaatagcc gacactgcag tggatgcagc ggatgttgca gcagatgttg cagcagatgc
     1561 aatagtcgac actgcagtgg ttgatatatt actgttgctt aatgcatttt ttaacgggcc
     1621 aggaaggtct ttaaaattaa aacctatttc atcatgcaga gggaatatct gtaaaaaata
     1681 attacctgat gtaggatgaa tatctggttt tacaatgttc atctgaacac catatttacc
     1741 agagatacca ggaacggtgc tgcgtatttc taatgccatc tgagtgacat tgaataaacc
     1801 aaacatatcc aacgcttttt tccctgcaga accttcttca agatgaggtg caagatattt
     1861 atccagccca gagagcagac cttcatcttg tatcaaactc aatagtgccg ttctttcacc
     1921 acattccgga gcatgttgct gtatatcatc tggaatatga acaacaaatc ccgcttcgca
     1981 tccattaggt aattcggatt gcggtatttg tgcagggggc gcttgttctg cctgattatg
     2041 ttgattgttt tgtgtggtga ttccggattg tacgatcggt tgaatgttca tgataaatcc
     2101 ttatgacgta atttatatcc attaataata tcaatccatt gatatt
//
LOCUS       DAZFYC010000301         2097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFYC010000301 DAZFYC010000000
VERSION     DAZFYC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.307"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            579..1352
                     /locus_tag="ACHKW5_005419"
     CDS             579..1352
                     /locus_tag="ACHKW5_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7514563.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFSIVQFPELNHQETFSAALWNSIIHFSI"
     gene            complement(1704..>2097)
                     /locus_tag="ACHKW5_005420"
     CDS             complement(1704..>2097)
                     /locus_tag="ACHKW5_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7514564.1"
                     /translation="EEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMSVLARELV
                     ASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDGEIRRYDR
                     QLNIICSECRNGEVMQRVLIFYTGVMME"
BASE COUNT          637 a          450 c          350 g          660 t
ORIGIN      
        1 cgtaagaggc taacctagat ttgccgttca tagattgagc ctcagattaa tgttaagcgt
       61 cttgcaggac gcgtaatgtt aactggggct tttctctatc tgccgttggt gttcatgccc
      121 gaggcagata gcctcaagca cccgcagcta ttctacttaa cttcccatta cctcgccaat
      181 atgaaatcgg tcagaaagac agacgtccca taaggacaac aacggaaaaa gagattttgc
      241 ttgatgctat ttacctctgc accatgaagt tgcgccaaaa tcccagtggt gataacagga
      301 atttggttgt tggtgattgg ggaaagaagt acgttagact gtgggttagt tcattgacat
      361 gatagttacc tgccttcaat ttcctaagca agcgatgtaa acgataatca atatcaaatg
      421 tattgacagg taaaataata acgatgtacc tttgctgata acatatatca gtcttaaatc
      481 ataatggagg tagataaaat aactaaacac acacaacaaa ttttttatca gtgacatagc
      541 atatcttaag catcctttaa tgctcatggg gagaacgcat gactaaacat atggacattc
      601 attgtcaaac acgcatattt aactcaatta accatgacac caaatataaa atatgtattt
      661 ttactccaaa aaacattaaa aaaaataatg caactcttta tatacttgat gggaatagtg
      721 ccaacaatta catttctgat attctgcctg ttattgatgc actacccaat ccaccagtgt
      781 tagttacact tggttatgaa tcttggaata accttagcat tcatcgccgt gcttacgatt
      841 atactcctga tggtgaaaat gctattgttg ataactctaa accagcatgg atctacttta
      901 ctggtggagg aagccagtct ttccgagaat tattactaac tcagatcatg ccatgggtta
      961 gtactattgc tccgaacagt tccagaatcg gtatatgggg gcactcgcta ggtgctattt
     1021 ttgtgcttga ttgcttaaaa aataattcat gctttaacta ttattatata tctgcaccat
     1081 cacttctatg gcagaatgaa agaatcatca agataattaa agatgatata tcagaatcaa
     1141 agcacactaa aagtatatgc ttgcttaacg gaaatcttag ccttgattac tccgcatctt
     1201 tatatcctga agccatcaaa gcggaatctg ttttgagaaa tattttgaca gaaaaataca
     1261 gtaatttttc tattgtacaa tttccagagc tcaatcatca ggagactttc tctgctgcac
     1321 tctggaatag cattattcat ttcagcatat aacagaaaat atgtaatcac tttatcaatc
     1381 tagctaacat aactgatatc aatcctaatg ggtctttgaa catattaaaa gataacttct
     1441 catttgtaca ttgttgtgtt cttagaccca tttcaaaaat actatttttt agatactaat
     1501 aattacatcc atttagctat cttctaaata ctcctctcag tttcttcatt ggagctgatt
     1561 caactccggc aatgcatact aggatcattt tataagcata caaacaacat ccagactgtg
     1621 aattattgct attcattttc tatgtatctg ctcttttccc atccactgaa acaccagaat
     1681 ccaaaggttt catgttattt tcactattcc atcattactc ctgtgtaaaa tatcaaaact
     1741 cgctgcatca cttcaccgtt acggcactca ctacaaatta tgttcaattg cctgtcatag
     1801 cggcgtattt ctccgtctgg taatgaccag ataaggtcag gatcaaccac aaccggtttc
     1861 ttcagttttg ccctcgatag ttttttgcgg gcgttttgcc agtccttacg agcctgttca
     1921 gatgggaata acccgtaacc agagttgtat acatcgccac tggcaaccag ctctctggca
     1981 agaacgctca tcagatatct tgtcgcacct gtcttggctt ccagttgccg taacgtctcg
     2041 cgcccactcc ggcgtactag ctcaacaacc tgccctttaa ttttttcccg ctcttcc
//
LOCUS       DAZFYC010000302         2072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFYC010000302 DAZFYC010000000
VERSION     DAZFYC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.308"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..74)
                     /locus_tag="ACHKW5_005421"
                     /pseudo
     CDS             complement(1..74)
                     /locus_tag="ACHKW5_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(76..258)
                     /locus_tag="ACHKW5_005422"
                     /pseudo
     CDS             complement(76..258)
                     /locus_tag="ACHKW5_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            complement(239..652)
                     /locus_tag="ACHKW5_005423"
     CDS             complement(239..652)
                     /locus_tag="ACHKW5_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7514565.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREKAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVADVSEDDVALMMASAGIPGGVRYVPAGW"
     gene            complement(679..1110)
                     /locus_tag="ACHKW5_005424"
     CDS             complement(679..1110)
                     /locus_tag="ACHKW5_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7514566.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSEAVRPDTDSTEVAKYNALTDDDFSAGKSSTAS"
     gene            complement(1124..1876)
                     /locus_tag="ACHKW5_005425"
     CDS             complement(1124..1876)
                     /locus_tag="ACHKW5_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514567.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWLRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRVVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(1884..>2072)
                     /gene="gpU"
                     /locus_tag="ACHKW5_005426"
     CDS             complement(1884..>2072)
                     /gene="gpU"
                     /locus_tag="ACHKW5_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO7514568.1"
                     /translation="AQVPDSELDSWMESRIYPAMTAIPALADLITTMVTQGYEYRRDD
                     DMALWSSADLTYSITYEM"
BASE COUNT          450 a          657 c          477 g          488 t
ORIGIN      
        1 attcgccgtg agccaaccag catttcaccg taaggcaccg gcatcgggtt cccctgggca
       61 atcatgttat ccagcggaaa tcccggcttt ttgtgcagcc agcgcctgac gactcaggtt
      121 ctgctcaacg acaccgacgg tttttctggc gtcagtctcc agtccggaaa aatgacgcct
      181 tacccggctc atctgctcat cgaaacggac cgcatccaga ctcaggtcaa taacaagatc
      241 accaaccggc tgggacatat ctcacacctc ccggaatccc cgctgaagcc atcattaatg
      301 cgacatcatc ctcgctgaca tccgccacat ccgcagacga taaaatctca cgccctccgt
      361 ccccaccaaa ccgtacgcct ccggcaagtc ctgccgcttt ctgcatcagc attttttcct
      421 catccggcat ctccgtctgc gctttctcac gccggggggc aagcagactg aaatcagagg
      481 gatgcatatc cggatcgcaa aaaaacaggc tgagtacggc gtacgtcagc ccggaaaaat
      541 gcatatccag ttgggtatcg tgaaaataat gcgtacggta aaaatgtcgc cagtcggcat
      601 attcggtgga tgtcatcccg gcaagcatgg cgcgccagtc aggcctcccc atctcacgcg
      661 ccagtctgag ggcaaaattc agctcgccgt cgaagacttt cccgcagaaa aatcatcatc
      721 agtcagcgcg ttatatttcg ccacttcggt gctgtcagta tccggacgaa cagcttcgct
      781 catcccggac agacgcaaca ccacatcttc cgcccgggca atggcatccg caggccaggt
      841 ggtgagcacc tcctgttcga tctgcatcac agcctcattc attgacggtg atgccgtttt
      901 ctgcggatgg ttatgccaca gggacatcgc caccagaaac gcgccggttc tgacgagatc
      961 ttccacgctt acctgcaggt tgccgctgga ttctgcctgt tctgcacgcc gtttcaggag
     1021 ggcaagatgc tcaatacgct gcagcgcaga caattcggaa agcgtgacgg acacaccgtt
     1081 atattcaaat tgttctgttt tcagaaacat gtattacctc cgtttaccct gcagcgcccg
     1141 cttcagtaac ggtgacttca gccactgcgg cgaactgacc atttccgctc accacaggga
     1201 tctgcacctt acctgtcgcc acgccgttta ccgtaattgt catatctttc acactaatgg
     1261 tggctttcga cggatcggcg gaaaccactc tgaacgtctt gtcggttgca ctttccggct
     1321 caaaagaaac cgtcagggtg gttgttttcc cttttgccac cgtaccggat gtcggcgtca
     1381 ccttaatcgc agtgaccggc gtaattttgc tgcgttcttc cgctacagaa ggtttgccca
     1441 cgttggtcac tttcaccgtg cgggtgatca cttctttcgc cgtcacggcc ttaccgatac
     1501 tgctgaccca gccacgaaac acatccaccg tgccgttcgg aaaacggatt ttataggccc
     1561 gcacatcccc gctttcaaac cagcctataa gccctttctg accttcttct cccggtttcc
     1621 aggccagcgt aaaactggta tctcctgcag acttctgccc ctgcccggtc gcggtccagt
     1681 ccgcgtcttc atcatccagg tagttatcat cgtagggttc tgccgtcatc tcgcccggcg
     1741 tcagatcctt caccttagcc agtcgcagcc agtcatcgtc tgacaacggg tttgcatagg
     1801 cgtcaccctg accgttataa acccacaggg tggtaccggc accttttacc ggctcaaggg
     1861 gatttggtgt tgccatatcg tcctcacatc tcgtatgtaa tggaataagt cagatccgca
     1921 gaactccata acgccatatc gtcatcacga cgatactcat agccctgcgt aaccatcgtc
     1981 gtaatcaggt ctgccagtgc cgggatcgca gtcatcgccg gataaatccg gctttccatc
     2041 cacgaatcca gctccgaatc cggtacctgt gc
//
LOCUS       DAZFYC010000303         2045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFYC010000303 DAZFYC010000000
VERSION     DAZFYC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.309"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..93
                     /gene="dicB"
                     /locus_tag="ACHKW5_005427"
     CDS             <1..93
                     /gene="dicB"
                     /locus_tag="ACHKW5_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7514569.1"
                     /translation="KRKHERELLNKICILSMLARLRPIQKGCWQ"
     gene            90..281
                     /locus_tag="ACHKW5_005428"
     CDS             90..281
                     /locus_tag="ACHKW5_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7514570.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            374..>2045
                     /locus_tag="ACHKW5_005429"
     CDS             374..>2045
                     /locus_tag="ACHKW5_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO7514571.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDECRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESYPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NTMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGSVKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQS"
BASE COUNT          633 a          478 c          494 g          440 t
ORIGIN      
        1 aagagaaaac acgaacggga gctattaaat aaaatatgca ttctttcaat gctggcccgt
       61 ttacgtccga tacaaaaagg atgctggcaa tgaatacagc atttgcactt gttctgacag
      121 tttttcttgt ttccggagag ccagttgata ttgcagtcag tgttcacagg acaatgcagg
      181 agtgtgtaac tgcagcaacc gaacagaaaa ttcccggtaa ctgttacccg gtcgataaag
      241 ttattcacca ggataataac gaaatcccgg caggtcttta aaacagttcc gtaataaaca
      301 tccgatttca ttcttatatg ccagcaatgg cagggatttg ttcaccctta aatctgtaat
      361 gaggtaaaac aaaatgagta aagtctttat ttgcgccgcc attccggacg aacaggcaat
      421 aaaggaagaa ggtgcagtcg ctgtagccac tgccattgaa gccggtgatg aatgtcgcgc
      481 ccgcgcaaaa tttcactggc aattcctgga acattatccg gctgctcagg actgcgctta
      541 taaatttatt gtctgcgagg ataaacctgg cataccccgc cctgccctcg attcatggga
      601 tgctgaatat atgcaggaaa accgctggga tgaggagtct gcttcttttg tcccggttga
      661 gactgaatcc tatccgatga acgtcacttt tgacaagctg gcccctgaag tacagaacgc
      721 tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt gatatggtga ttagcgcaca
      781 ggaattgttg caggaagaca tggcaacatt cgacggacat atcgttgaag cgttgatgaa
      841 aatgccagaa gttaacacca tgtatccgga gcttaagttg cacgccattg ggtgggttaa
      901 gcataaatgt attcctggtg ctaaatggcc cgaaattcag gcagagatgc gcatctggaa
      961 aaaacgtcgc gaaggtgaac gcaaggaaac cggaaaatac acgtctgttg ttgatctcgc
     1021 ccgcgccaga gccaatcaac agtacactga aaattcaaca ggaaaaatca gcccggtcat
     1081 tgctgccatt catcgcgaat acaagcagac atggaaaaca ctggatgacg aactggccta
     1141 cgctctctgg cctggtgatg tggatgccgg aaacattgac ggcagcatcc atcgctgggc
     1201 aaaaaatgaa gttatcgaca acggccgcga agactggaag cgtatctcgg catcaatgcg
     1261 caaacagcct gatgcccttc gctacgaccg ccagactatt tttggccttg tccgtgaacg
     1321 tccgatcgac attcacaaag atcccgtagc actgaacaaa tatatctgcg aatacctgac
     1381 gacaaagggc gtgtttgaga atgaagaaac agacctgggc actgttgatg ttctccagtc
     1441 atcagaaaca caaactgatg cagtggaaac tgaggtatct gatatcccaa aaaatgaaac
     1501 cgcgccggaa gctgaaccat ctgtagagcg tgaggggccg ttctacttcc tcttcaccga
     1561 caaggatggc gaaaaatacg gtcgcgcaaa caaactttct ggtctggata aggcgctgtc
     1621 tgctggggct actgaaatca cgaaagaaga atatttcgcc cgcaaaaacg gtacatactc
     1681 aggttcacaa caaaatactg gtgcatctga cacgaccgca caaccagggt cagtaaaagt
     1741 taccgctgac gaagtaaaca aaattatgca ggcagccaat atcagccagc ctgacgccga
     1801 tgaactgctt gcagtatcac gtggtgaatt tgttgaaggg attagcgacc cgaatgatcc
     1861 gaaatgggtt aaggggatcc agacccgcga ttctgtgaac cagaaccagc aagaaacgga
     1921 acagaacgac cagaaagcgg aacaaaacag cccaaatacg caacaaaacg agccagaaac
     1981 gaaacaacct gaaccagtag tgcaacagga accggaaaag atctgcaccg cctgcggtca
     2041 gagcg
//
LOCUS       DAZFYC010000304         2037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFYC010000304 DAZFYC010000000
VERSION     DAZFYC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.310"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..689
                     /locus_tag="ACHKW5_005430"
     CDS             144..689
                     /locus_tag="ACHKW5_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7514572.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            664..>2037
                     /locus_tag="ACHKW5_005431"
     CDS             664..>2037
                     /locus_tag="ACHKW5_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7514573.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMS"
BASE COUNT          495 a          488 c          592 g          462 t
ORIGIN      
        1 ttaaggcgtt tccgttcttc ttcgtcgtaa cttaatgttt ttatttaaaa taccccctga
       61 aaagaaagga aacgacaggt gctgaaaacg aacttttggg cctttgtcgt ttcctttctc
      121 tgtttttggc cgtggaatga acaatggaag tcaacaaaaa gcagctggct gacattttcg
      181 gtgcgagtat ccgtaccatt cagaactggc aggaacaggg aatgcccgtt ctgcgaggcg
      241 gtggcaaggg taatgaggtg ctttatgact ctgccgccgt cataaaatgg tatgccgaaa
      301 gggatgctga aattgagaac gaaaagctgc gccgggaggt tgaagaactg cggcaggcca
      361 gcgaggcaga tctccagcca ggaactattg agtacgaacg ccatcgactt acgcgtgcgc
      421 aggccgacgc acaggaactg aagaatgcca gagactccgc tgaagtggtg gaaaccgcat
      481 tctgtacttt cgtgttgtcg cggatcgcag gtgaaattgc cagtattctc gacgggctcc
      541 ccctgtcggt gcagcggcgt tttccggaac tggaaaaccg acatgttgat ttcctgaaac
      601 gggatatcat caaagccatg aacaaagcag ccgcgctgga tgaactgata ccggggttgc
      661 tgagtgaata tatcgaacag tcaggttaac aggctgcggc attttgtccg cgccgggctt
      721 cgctcactgt tcaggccgga gccacagacc gccgttgaat gggcggatgc taattactat
      781 ctcccgaaag aatccgcata ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc
      841 atcatgaatg cgatgggcag cgactacatc cgtgaggtga atgtggtgaa gtctgcccgt
      901 gtcggttatt ccaaaatgct gctgggtgtt tatgcctact ttatagagca taagcagcgc
      961 aacaccctta tctggttgcc gacggatggt gatgccgaga actttatgaa aacccacgtt
     1021 gagccgacta ttcgtgatat tccgtcgctg ctggcgctgg ccccgtggta tggcaaaaag
     1081 caccgggata acacgctcac catgaagcgt ttcactaatg ggcgtggctt ctggtgcctg
     1141 ggcggtaaag cggcaaaaaa ctaccgtgaa aagtcggtgg atgtggcggg ttatgatgaa
     1201 cttgctgctt ttgatgatga tattgaacag gaaggctctc cgacgttcct gggtgacaag
     1261 cgtattgaag gctcggtctg gccaaagtcc atccgtggct ccacgccaaa agtgagaggc
     1321 acctgtcaga ttgagcgtgc agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc
     1381 tgcccgcatt gcggggagga gcagtatctt aaatttggcg acaaagagac gccgtttggc
     1441 ctcaaatgga cgccggatga cccctccagc gtgttttatc tctgcgagca taatgcctgc
     1501 gtcatccgcc agcaggagct ggactttact gatgcccgtt atatctgcga aaagaccggg
     1561 atctggaccc gtgatggcat tctctggttt tcgtcatccg gtgaagagat tgagccacct
     1621 gacagtgtga cctttcacat ctggacagcg tacagcccgt tcaccacctg ggtgcagatt
     1681 gtcaaagact ggatgaaaac gaaaggggat acgggaaaac gtaaaacctt cgtaaacacc
     1741 acgctcggtg agacgtggga ggcgaaaatt ggcgaacgtc cggatgctga agtgatggca
     1801 gagcggaaag agcattattc agcgcccgtt cctgaccgtg tggcttacct gaccgccggt
     1861 atcgactccc agctggaccg ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa
     1921 agctggctga ttgaccggca gattattatg ggccgccacg acgatgaaca gacgctgctg
     1981 cgtgtggatg aggccatcaa taaaacctat acccgccgga atggtgcaga aatgtcg
//
LOCUS       DAZFYC010000305         2036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFYC010000305 DAZFYC010000000
VERSION     DAZFYC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.311"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..141
                     /gene="dicB"
                     /locus_tag="ACHKW5_005432"
     CDS             <1..141
                     /gene="dicB"
                     /locus_tag="ACHKW5_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7514574.1"
                     /translation="IGVLLSNREFTEDAIKMRKYEPYLLNDNSILSRIALLELGIIGE
                     QQ"
     gene            138..326
                     /locus_tag="ACHKW5_005433"
     CDS             138..326
                     /locus_tag="ACHKW5_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7514575.1"
                     /translation="MTSAFALIMTVFLITGEPQNVITGIYYSKSSCIQVRDEQKIPGE
                     CLPLKKVSLYLNNETPAG"
     gene            419..>2036
                     /locus_tag="ACHKW5_005434"
     CDS             419..>2036
                     /locus_tag="ACHKW5_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO7514576.1"
                     /translation="MNADKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VAAMDDAGFDEEDFKKPIRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMILIAPS
                     VPDKPASTDNTNVNGEDMAKIEENMLLPVSGQILPVRWLAQHNSEKPLTHVSLDKLRA
                     LHNAQDEKLPAVTALAISNKAVQLEPLELRDLYKLVRDTDKVFPAPVNSDLGLMTSFI
                     EAYLGADYTDRGLLTKEWMKGNRVSRISRTPSGANAGGGILTDRGEGFVHDDASVARD
                     VATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFFSKFITMPGHKDYSRAIVV
                     ASVKEAPIGIEATPHRVTEYLNKVLTETDHATPDPEIVDIACGRSSAPMPQRVTKEGK
                     QDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAELH
                     EAKKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDDDDQNQDKAEQNS
                     PNALQNEPKTKQPEPVVQQEPEKVCTTCGQT"
BASE COUNT          621 a          487 c          475 g          453 t
ORIGIN      
        1 attggagttc tgctcagtaa ccgggagttt actgaagatg ccattaagat gagaaaatat
       61 gaaccttatc ttcttaatga taattccata ctttccagaa ttgcccttct tgaacttggt
      121 attatcggag aacagcagtg acttcagcgt ttgcactgat aatgaccgtt tttcttataa
      181 cgggtgagcc gcagaatgtg attaccggaa tttattacag taagtcatcc tgcattcagg
      241 taagggacga acaaaaaatc cccggtgaat gcctcccgtt aaaaaaagta tcgctgtacc
      301 tgaataacga aacaccggct ggataacccg ccagccatat taacgccata ccaacggatt
      361 aaaaatgcca gcaatggcag gaatttgttc atccttaaaa tggttatgag gtttatcaat
      421 gaacgctgat aaagaagaaa ttgcactata ttacgaagcc aaaaatgaca aagtcagaaa
      481 acgccttggg attaaaggtg gtttttactg gcgcacagca aaaaaattat cggttgcaat
      541 atcacgtggt gttgctgcaa tggacgatgc cggatttgac gaagaggatt ttaaaaaacc
      601 catccgcgtc catttacccg ttgtgaatga ccttccaccg gaaggtgtgt ttgatactga
      661 attctgcaac cgatacgaaa aaggcggaga agatggtatc acaatgatac ttatcgcgcc
      721 ctctgttccg gacaaaccag ccagtactga caataccaac gtcaacggcg aagacatggc
      781 taagattgag gagaatatgc ttcttccggt ttcaggtcag attctgcctg ttcgctggct
      841 ggcgcaacac aacagcgaaa aaccgctcac gcacgtttca ctggacaaac tgcgcgcatt
      901 acataacgca caggatgaaa aacttcccgc cgttaccgcg ctggccatct caaataaagc
      961 agtgcaactc gaaccgctgg agcttcggga tttgtacaaa ctggtgcgag acactgacaa
     1021 agttttcccc gcccccgtaa attcggacct gggactgatg acctctttta tcgaagcata
     1081 cctgggcgct gactacaccg atcgcggtct gcttacaaaa gagtggatga aaggaaatcg
     1141 tgtttcacgc atcagccgca cgccatccgg cgctaacgct ggcggcggca ttcttaccga
     1201 tcgcggcgaa ggttttgtcc acgatgatgc gtcagtggcg cgtgacgtag ccactggcgt
     1261 actggcccgt tcaatggacg tggatattta taaccttcat cccgcgcacg ctaaacgcat
     1321 tgaggaaatt atcgctgaaa ataaaccgcc attttctgtg tttttcagca aattcatcac
     1381 catgccgggc cacaaggatt actcccgcgc catcgtggtt gcgtccgtga aagaagcacc
     1441 aattggtata gaggctactc cccaccgtgt caccgaatac ctgaataagg tgctgactga
     1501 aaccgatcat gccacccctg atccggaaat cgtggatatt gcctgtggtc gctcctctgc
     1561 cccgatgccg cagcgtgtaa caaaagaagg aaaacaggat gatgaagaaa aaccgcagcc
     1621 atctggcgca atggcagatg aacaggcaac gactgaagca gtggaaccgg atacaactga
     1681 acataatcag gacacgcagt cgatggatgc tcagccacag ataaattctg ttgatgcgaa
     1741 atatcagaaa ctgcgtgcag aactccatga agccaagaaa aacattccac ccaaaaatcc
     1801 tgtcgatgca gataaattac tggctgcttc tcgaggagaa tttgttgaag ggattagcga
     1861 cccgaatgat ccaaaatggg tgaaggggat ccagagtcgc gatgatgatg accagaatca
     1921 ggataaagcg gaacaaaaca gcccaaatgc gttacaaaac gagccaaaaa caaaacaacc
     1981 tgaaccagta gtgcaacaag aaccagaaaa agtttgcacc acatgtggtc agaccg
//
LOCUS       DAZFYC010000306         2017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFYC010000306 DAZFYC010000000
VERSION     DAZFYC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.312"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..146)
                     /gene="hokD"
                     /locus_tag="ACHKW5_005435"
     CDS             complement(<1..146)
                     /gene="hokD"
                     /locus_tag="ACHKW5_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7514577.1"
                     /translation="MKQQKAMLIALIVICITVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEP"
     gene            complement(392..496)
                     /locus_tag="ACHKW5_005436"
     CDS             complement(392..496)
                     /locus_tag="ACHKW5_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514578.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(612..1196)
                     /locus_tag="ACHKW5_005437"
     CDS             complement(612..1196)
                     /locus_tag="ACHKW5_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7514579.1"
                     /translation="MITFTKEQLISHVSENVKAMKFAVKQTVFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     NKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(1253..1648)
                     /locus_tag="ACHKW5_005438"
     CDS             complement(1253..1648)
                     /locus_tag="ACHKW5_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7514580.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKATDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(1664..>2017)
                     /locus_tag="ACHKW5_005439"
     CDS             complement(1664..>2017)
                     /locus_tag="ACHKW5_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7514581.1"
                     /translation="SGGNLPADPKAAPVTKNDGKAFPQPAGAALPVREAATQEEIKTE
                     TVADIVQPLLSFTETQADELIFPSLRRANLALRRAKSDVQKWERVCAALRELNKHRDI
                     VRQITDSSRRVVSEK"
BASE COUNT          479 a          562 c          429 g          547 t
ORIGIN      
        1 ggttcgtaag ctgtgaagac agcgacctcc gtctggccgg ttcggattcg tacctcgcag
       61 aggtctttcc tcgttaccag tgccgtcact atgacggtga tacagatgac gatcagggcg
      121 attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcgg cgcgtaagag
      181 gctaacctac atatgtctag catgaaattg gcctcagatt aatgttaggc gtcttgcagg
      241 acgcgtaatg ttaactgggg cttttctctg tctgccttac ggtggcatgc ccgaggcaga
      301 cagcctcaag cacccgcagc aattctactt aactctcgct ttaccgcaaa ccgtttttac
      361 ccgatatggg aattcccata tcgtaatgaa ttcagttccc tagtcgatcc atcaaaaaca
      421 caaccaggca gtaaacgccc acaacagcaa taacagccag agcaccttcc attgccagtg
      481 aaatatcatc cgacatattc cctcctttgg tgtgaatccc ggcgaacgtt tttaccccca
      541 ccgacaaata acatatacta aaaaatcaat agctatagca acgcctgtaa ttgcaaaggc
      601 ttcaggccag atcattggcg cacctcctgc ggcggttctg gtagcggcat ccagtcggtt
      661 acattgcggc tctgtgtttc gaaaaattca tcaccattac ggactacatc aaaaaactca
      721 ccgtctcgat attgcgcata aagaacgaat gcgccatcac ataaaataat tacgtgctga
      781 ccatcatccg gcattcgctc actacagctt atccaaccat ccggagttac cggatagttg
      841 gttgacgttt ccgagatttc ccgaaaatta ttggttgacg aacccttatt ttcccgaaag
      901 tttccagcct gaagcatggc ggcgctgtct ggcggggcag catatagcgg cacgtatatt
      961 tccggttcct tatcagcacc gggttgctct tccagtgaga atgtctttcc ggtaaatcga
     1021 ttcatataaa gcacgggctc tgcttccagc gaagcctgag caacaagggc cagtgctaaa
     1081 tccaactcaa ttgcctcgag agaatttttg aatactgtct gttttactgc aaatttcatc
     1141 gcctttacat tttcactaac atgactgatt aactgctctt ttgtaaaagt gatcatctca
     1201 ttctcctttg atgcgaatgc cagcgacaat tgaagcctga tagctaattc actcacagta
     1261 ccgcctcctg aaaattgccc tgatagaacg ccagtacacg cagcataact tcactcttcc
     1321 ggcactcgcc acagattatg ttctgttgtc tgtcgtagcg gcgtatttct ccgtctggta
     1381 actttcgaat caatgtctgg tcggttgctt tctccgctgc cttacgccat acgcgataca
     1441 cctgttctga tgtaaaaaca ccgtatttac cgggcatgta taaatcgcca caagccagta
     1501 catccacaag gcaacgtctg actgaatgcc agcctgctcc cgtcgctctc tccagttgtg
     1561 atatcgtcat gcgttcattt ttgcgtacca gcccgataat tcgggccttc agttcttcac
     1621 gctgttcgtg tgtaaaaggt ttcgccataa gcgcctccgg ctatcacttt tccgatacaa
     1681 cacggcggga agaatcagta atctgtcgaa caatatcccg gtgcttgttc agctcccgca
     1741 gcgcggcgca gactcgctcc cacttctgaa catcactttt cgccctgcgc agcgccaggt
     1801 ttgccctgcg cagggacgga aaaatcagct catctgcttg cgtttcggta aacgatagca
     1861 acggctgcac aatgtccgcc acagtttctg ttttaatttc ttcctgtgtt gcggcttccc
     1921 ggactggtaa cgcagcacct gctggctgag gaaaggcctt accatcattt ttcgttaccg
     1981 gcgcggcttt cggatctgct ggtaaattac cccccga
//
LOCUS       DAZFYC010000307         1997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFYC010000307 DAZFYC010000000
VERSION     DAZFYC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.313"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1997
                     /locus_tag="ACHKW5_005440"
     CDS             <1..>1997
                     /locus_tag="ACHKW5_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO7514582.1"
                     /translation="EYYSHTVDIEKNIHAIWVAGSPPESISDYIKTFLKTYKEFTYYL
                     WVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNEIRKKYNNNPSGQQ
                     EYLNKLRELYATYQKISTPLKHMFNSFFLENMIKLQDNFFNYCIVKGVTEINDELRIN
                     YLKNVIKLSDDDIGNYQKTINDNKDRVKKLILDLQKQFGENRISIKDVNSLTSLSKSE
                     NNHNYQTEMLLRWNYPAASDLLRMYILKEHGGIYTDTDMMPAYSKQVIFKIMMQTNGD
                     NRFLEDLKLRRAISDGVLRYVNNQNIDEVNYNEISDADKNIIKKILTEISKMPEDSIF
                     TKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELR
                     RIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQ
                     ISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTTGTGKYDWLTPESIGV
                     NDVTPADESTWCIGKGRCVDDFLFKDVSTLKTENLPELFLTKIDTDTFFSQWSTKTKK
                     DLQKKIQDLTVRYNELIDSSTIDFKNLYEIDQMLHMIMLEMNDDIAKRSLFSLQVQIA
                     EKIRRMTIPVDNIINIYPDLHKKNDNDLSMSIKGFLASNPH"
BASE COUNT          745 a          335 c          348 g          569 t
ORIGIN      
        1 ggagtactat tcgcatacag ttgatattga gaaaaatatt catgccatat gggttgcggg
       61 ttccccacct gaaagcatct cggactatat taagactttt ctcaaaacct acaaagagtt
      121 tacttactat ctttgggttg atgaaaaagc atttggagcc gcgaaattta ccagtgtctt
      181 aaaacaaatt gcatttgatt tagcatgcag aactatacag cagaatactc cacaaaaaaa
      241 tattgatttt attaatctat ataatgaaat aaggaagaaa tacaacaaca acccatcggg
      301 acaacaagag tacttaaaca aactcagaga gctttatgct acttatcaaa aaatatccac
      361 ccctctgaaa cacatgttta attcattttt tttagaaaac atgattaaac ttcaggataa
      421 tttcttcaac tattgcattg tcaaaggtgt tacagagatt aatgatgagt taagaataaa
      481 ctaccttaaa aatgtaataa aactgtcaga cgatgacatt ggtaattatc agaaaacaat
      541 taacgacaat aaagatagag taaaaaaact aattcttgat ttacagaaac aatttggtga
      601 aaaccgcatt tcaattaaag atgttaactc tttaacctct ctttcaaaat cagaaaataa
      661 tcacaattat caaactgaaa tgttgcttcg atggaactat cctgccgcct cagacctgct
      721 caggatgtat atccttaaag agcatggtgg tatttataca gacacagata tgatgcctgc
      781 atactctaaa caagtaattt ttaaaattat gatgcagaca aacggagata atcgtttctt
      841 ggaagatttg aaactacgtc gtgcaatatc tgatggtgta ttaagatatg ttaataacca
      901 aaatattgat gaagttaact ataatgaaat cagtgatgca gataaaaaca ttatcaagaa
      961 gatattaaca gaaatatcta aaatgccaga agatagtatt tttactaaga tcaatacaag
     1021 gattcctcga gacacaatgc ccatccttcg tcgttatcac ctatggcctg atggatggaa
     1081 tattcgtggg ctcaatggat tcatgctatc acataaaggt agtgaagtga ttgatgctgt
     1141 tatcgcaggc cagaatcagg cttacaggga actaagaaga ataagagata atattcatag
     1201 tgaaatatac ttcaaacaaa ctgatgaatt gtcctcactt ccagatacag acaaaattgg
     1261 agggattctg gtaaaaaaat acctttcagg aagtcttttt tcaaaattca gacaagatac
     1321 tattatcccg gaggcattga gcaccctcca gatatcaggt cctgacttga ttcaaagaaa
     1381 gatgttgcaa tttttcagga gtagaggggt gttaggtgaa gagttcatta atgaaagaaa
     1441 actgagtgat aaagcttata ttggtgtcta caaaacaact ggcacaggga aatatgactg
     1501 gttaacccct gaatcgatcg gcgttaatga tgtcacgccc gcagatgaaa gtacctggtg
     1561 tataggaaaa ggccggtgtg ttgatgactt cctgttcaaa gatgtttcaa cactaaaaac
     1621 agaaaatctt ccagaattat tcttaacaaa aatagatact gatacgtttt tctctcagtg
     1681 gtcaaccaaa accaagaaag atctgcaaaa aaaaatacaa gaccttactg tacgttataa
     1741 tgagctaatt gactcgtcga ctatcgactt taaaaatcta tatgaaatag atcaaatgct
     1801 ccatatgatt atgctagaga tgaatgatga tatagccaaa aggtcattgt tttcattgca
     1861 agttcaaata gccgaaaaaa ttcggaggat gaccattcct gtagacaata taattaacat
     1921 ctatcctgat ctacataaaa aaaatgacaa tgatctgagt atgtccataa aaggctttct
     1981 tgcgagtaat ccacata
//
LOCUS       DAZFYC010000308         1973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFYC010000308 DAZFYC010000000
VERSION     DAZFYC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.314"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1376
                     /locus_tag="ACHKW5_005441"
     CDS             <1..1376
                     /locus_tag="ACHKW5_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7514583.1"
                     /translation="GRLTEKTDLIPEGGIRTDDERTHRYHYDSQHRLVHYTRTQYAEP
                     LVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRI
                     QTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRL
                     ESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVST
                     EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPD
                     IAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGM
                     DTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQR
                     TSRGIRK"
     gene            1373..1942
                     /gene="ybfC"
                     /locus_tag="ACHKW5_005442"
     CDS             1373..1942
                     /gene="ybfC"
                     /locus_tag="ACHKW5_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO7514584.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIEIQHELIRSEEK"
BASE COUNT          579 a          447 c          520 g          427 t
ORIGIN      
        1 acggcaggct gacggagaaa accgacctca tcccggaagg gggtatccgt acggatgatg
       61 agcgcaccca ccggtaccat tacgacagtc agcaccggct ggtgcactac acgcggacac
      121 aatatgcaga gccgctggtc gaaagtcgct atctttacga cccgctgggc cgcagggtgg
      181 caaaacgggt gtggcggcgt gaacgggacc tgacgggctg gatgtcgctg tcacggaaac
      241 cgcaagtgac ctggtacggc tgggacggcg accggctgac cacgatacag aacgacagga
      301 gccgcattca gacgatttat cagccgggga gcttcacgcc actcatcagg gttgaaaccg
      361 ccaccggtga gcaggcgaaa acgcagcgcc gcagcctggc ggataccctt cagcagtccg
      421 gcggcgaaga cggtggcagt gtggtgttcc cgccggtgct ggtgcagatg ctcgaccggc
      481 tggaaagtga aatcctggct gaccgggtga gtgaggaaag ccgccgctgg ctggcatcgt
      541 gcggcctgac ggtggagcag atgcaaaacc agatggaccc ggtgtacacg ccggcgcgaa
      601 aaatccacct gtaccactgc gaccatcgcg gcctgccgct ggcgcttgtc agcacggaag
      661 gggcaacaga atggtgcgca gaatacgatg aatggggcaa cctgctgaat gaagagaacc
      721 cgcatcagct gcagcagctt atccgcctgc cggggcagca gtatgatgag gagtccggcc
      781 tgtattacaa ccgccaccgc tattatgacc cgctgcaggg gaggtatatc actcaggatc
      841 cgattgggct gaagggggga tggaattttt atcagtatcc gctgaatccg gttcagtata
      901 tagattcaat gggactggca tcaaaatatg gacacttaaa taatggcgga tatggagcga
      961 gacccaacaa accgcctacg cccgatccaa gtaaattgcc ggacatagcg aaacaattaa
     1021 gactgccata tcctattgac caggccagta gtgcgcctaa tgttttcaaa acattcttca
     1081 gagcattaag cccttacgac tacacactgt attgcaggaa gtgggtaaaa ccaaatctga
     1141 cttgtacgcc acaggatgat tcccagtatc cagggatgga tacaaagaca gcaagtgatt
     1201 acctgccaca gacaaattgg ccaacaactc aattaccacc aggatatact tgtgcagaac
     1261 cctatttatt cccagacatt aataaacccg atgggccagc aacagcaggg atagatgatt
     1321 tgggtgaaat tttagctaag atgaaacaga gaacatcgag aggaataaga aaatgaaaag
     1381 agttttgttc tttttgctga tgatatttgt tagttttggt gttatagctg attgcgaaat
     1441 acaagctaaa gatcatgatt gttttactat tttcgctaag ggtactattt tttccgcgtt
     1501 tcctgtctta aataataaag ccatgtggag atggtatcag aatgaggaca tcggtgagta
     1561 ttattggcaa acagaactgg gtacatgtaa aaataataaa ttcacaccaa gcggggcgag
     1621 actcttaatt cgcgtcgggt cactacgcct taatgaaaat catgccatca aaggaacgtt
     1681 gcaggagttg ataaatacag cagaaaaaac ggcgtttctc ggcgatcggt ttagaagtta
     1741 tataagagcg ggtatctatc aaaaaaagag cagtgatcca gttcagttgt tggcagtcct
     1801 tgataattca atcatggtaa aatattttaa agatgagaaa ccaacttatg ctcgaatgac
     1861 agcacatctc ccgaacaaaa atgagtctta cgaatgttta atagagatac agcatgaact
     1921 aattcgtagt gaagagaaat aaaatctctt cctgagaaaa caaattaatc att
//
LOCUS       DAZFYC010000309         1946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFYC010000309 DAZFYC010000000
VERSION     DAZFYC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.315"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..409
                     /gene="rrrD"
                     /locus_tag="ACHKW5_005443"
     CDS             <1..409
                     /gene="rrrD"
                     /locus_tag="ACHKW5_005443"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7514585.1"
                     /translation="NDGLEGVSYIPYKDIVGVWTVCHGHTGKDIMPGKTYTEAECKAL
                     LNKDLATVARQINPYINVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKSAC
                     DQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            406..843
                     /locus_tag="ACHKW5_005444"
     CDS             406..843
                     /locus_tag="ACHKW5_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7514586.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDRITAQVNYLQEYIRTQCLK"
     gene            993..1610
                     /locus_tag="ACHKW5_005445"
     CDS             993..1610
                     /locus_tag="ACHKW5_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7514587.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAIMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(1798..>1946)
                     /locus_tag="ACHKW5_005446"
     CDS             complement(1798..>1946)
                     /locus_tag="ACHKW5_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7514588.1"
                     /translation="FPNQMIEQINIALEQKGSGNFSAWVIEACRRRLTSEKRAYTSIQ
                     SDDG"
BASE COUNT          535 a          451 c          491 g          469 t
ORIGIN      
        1 taacgatggt ctggaaggtg tcagctacat accatacaaa gatatcgttg gcgtatggac
       61 tgtatgtcac ggacacaccg gaaaagacat catgcccggt aaaacgtata ccgaagcaga
      121 atgcaaagcc ctcctgaata aagaccttgc cacggtcgcc agacaaatta acccgtacat
      181 caacgtcgat ataccggaaa caacgcgcgg cgctctttac tcgttcgttt acaacgtggg
      241 cgctggcaat ttcagaacat cgacgcttct tcgcaaaata aaccagggcg atatcaaaag
      301 cgcatgtgat cagctacggc gctggacata cgctggcggt aagcaatgga aagggctgat
      361 gactcgccgt gagattgagc gtgaagtctg tttgtggggg caacaatgag cagagtaacc
      421 gcgattatct acgttctggt catctgcctc atcgtctgcc tttcatgggc tgttaatcat
      481 taccgtgata acgccatcgc ctacaaagag cagcgcgata aagccacatc catcatcgct
      541 gatatgcaga agcggcaacg tgatgtagca gaacttgacg ccagatacac aaaggagctt
      601 gctgatgcta atgcgactat cgaaagtctc cgtgctgatg tttctgctgg gcgtaagcgc
      661 ctgcaagtct ccgccacctg tgcaaagtca acgaccggag ccagcagcat gggcgatgga
      721 gaaagcccag gacttacagc agatgctgaa ctcaattatt accgtctccg aggtggaatc
      781 gacaggataa ccgcgcaggt taactacctg caggaataca tcaggacgca gtgcttaaaa
      841 taattttaat ttcactgaaa tttaacaagt gactttcagg aaaatgcctc gcagatgcgg
      901 ggcatttttg taccggtatt tcaccgcgca ccgcagcgca caataaacac cgaacctgac
      961 cctttggaat gggcctttga ggataccagt tagtgctggc gagcctcggt gggctggttt
     1021 cctgtgcggc aaaggttcat ttcaaagaag caggcaacgc catgaatgaa ttaattgcga
     1081 atcatgactt cgactttcgc cagttagtta ccgcagcaga aggtcaaccg gtaactgaca
     1141 ccttccagat tgccagggca tttggtaaac gccatcagca tgtgattagg gctattaaat
     1201 gtttgagatg ttctgaggaa ttctcgacaa cccatttttg ggccgtcgag aaaatcaatg
     1261 acttagggat ttttgacaag aaacagattt actaccgcat ggactttagt ggcttcgtta
     1321 tgctggttat gggatttaac ggggcaaaag ccgatgctgt taaagaagcc tatatcaatg
     1381 cgtttaactg gatgtcagca gaactccgta agtacagcga aagttatgaa gcagaacgca
     1441 acgccataat gctggagtat atgaaagaga aggatgtcgc cagcatgtca ggccgtctgc
     1501 tcaatcgctg ggggagaacg aaaaaacctc aattgcttgc aaagctggaa cgtctggaga
     1561 gacagggaca gtttttatta ccgggattcg ataaaggtat tcaagcctga cacattatgc
     1621 gctgtatcgt cgccgtattc ccgcattaac catgaccgta gcccgacggg gaattccttc
     1681 tgcgtgagtg tgcgggaata atcaaaaacg atgcacaccg ggttttactg tgctgacaga
     1741 cgcagggtta ccctcatagt cgcttttccg gtgcgatggt ggaagaaacc gggatgttca
     1801 tccatcatca ctttggattg atgtatatgc tctcttttct gacgttagtc tccgacggca
     1861 ggcttcaatg acccaggctg agaaattccc ggaccctttt tgctcaagag cgatgttaat
     1921 ttgttcaatc atctggtttg gaaatc
//
LOCUS       DAZFYC010000310         1870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFYC010000310 DAZFYC010000000
VERSION     DAZFYC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.316"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..373
                     /locus_tag="ACHKW5_005447"
     CDS             <1..373
                     /locus_tag="ACHKW5_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7514589.1"
                     /translation="EEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIRRLSGALA
                     ASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQNIICRECR
                     QSEVMQRVLAFYRGNFQEVME"
     gene            370..666
                     /locus_tag="ACHKW5_005448"
     CDS             370..666
                     /locus_tag="ACHKW5_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO7514590.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            663..1277
                     /locus_tag="ACHKW5_005449"
     CDS             663..1277
                     /locus_tag="ACHKW5_005449"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7514591.1"
                     /translation="MREVNYEALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLELLDELEAQSKRIAELETNL
                     AALAAENAGLNKFIVQSCYVFDGEQDELSDAYICAIDGRMPQIPATDAFLADVRAGAF
                     NDLCAAFVRHAKIAGLDDGDLVTVKEATDALLHCAEQLREGFKQ"
     gene            1274..1558
                     /locus_tag="ACHKW5_005450"
     CDS             1274..1558
                     /locus_tag="ACHKW5_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514592.1"
                     /translation="MSESKCQINGNQIEPCAALSKSLEYGNPTFKSKGIFIPERVNIN
                     TGESGLDIAQIHSGKYIGRGVAMCFCPFCGESLKTWEETSEQYKKSWHEI"
     gene            1524..>1870
                     /locus_tag="ACHKW5_005451"
     CDS             1524..>1870
                     /locus_tag="ACHKW5_005451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514593.1"
                     /translation="MSNTKNHGMKFKGTPGPWHVSFHGSANCWVVDSERDKAIAKVTE
                     YNNDWQMQKANFQLLAAAPELLEALQAVVRVADRKTDEFEMAHSAINKALGVILMNVI
                     KEIPVMRDEYGCWT"
BASE COUNT          522 a          363 c          557 g          428 t
ORIGIN      
        1 agaagagcgg gaagaagtga aggcgcgcat tgtggaattc gtgcgcctga gcggacgaga
       61 aacttttcga caactggcag ataaaacggg tgtcagtaag accgctattc gtcgtttatc
      121 tggtgcgctt gcggccagtg gtgatgtctg gctctctggt tgcggggtat ttccatcaga
      181 gcaggcgtat cgcgtatggc gtaagacacc ggagaaggct gctgacccga cactgattcg
      241 aaagttacct gacggagaaa tacgtcgtta caacagacgg cagaacataa tttgtcgtga
      301 gtgccgccag agcgaagtta tgcagcgtgt gctggcgttc tatcggggaa actttcagga
      361 ggtgatggag tgagggtgag agtttatatt gccggtccaa tgacgggata tgaaaatttc
      421 aaccgtgagg cgtttcacaa ggcggaagag gaactgaaac gggaagggca taccgtctta
      481 aacccggcag tacttccgga cgggctgaca cagccgcact acatggatat ttgcatggca
      541 atgattcgtt gcgtggatgc gatttacatg ctgaatggct ggcagcggtc agcgggcgct
      601 aaggcagagc tggcactggc ggagaaactg ggacatgcgg tgatttatca ggaggtggct
      661 caatgagaga ggttaactat gaggcgcttc gtgaagcagc agagaaagca actaaaggat
      721 gctacatcgt agggcataca tcgggcaatc agcatgggaa tataacagga gtttttgttt
      781 gtcaaaaatg gaaaggagaa cccggtggcg tgattgcgga atgtcatgtt aactgcctga
      841 ttgaatcaga tgctcaggct tatgcaaacg ctgaattcat agcagaggct aacccggcta
      901 ccgtgctgga attactggat gaactggaag cccaaagcaa acgcattgca gagctggaga
      961 ctaatcttgc tgcgcttgcg gcggagaatg cggggctgaa taaatttatc gtacagagtt
     1021 gctacgtgtt tgatggcgag caggatgaac tatctgatgc gtatatctgt gcaatagacg
     1081 gaaggatgcc gcaaatccca gccaccgacg ctttcctggc ggatgtgcgg gctggggcgt
     1141 ttaacgacct ttgcgcggcg tttgtcaggc acgcaaaaat tgcaggactg gacgatggcg
     1201 atctcgttac ggtgaaagaa gcgactgatg cgctgctgca ctgtgcagaa cagcttcggg
     1261 agggattcaa gcaatgagtg aatcaaaatg tcagattaat ggcaatcaga tagagccgtg
     1321 cgcggcgttg tcaaaatctc tcgaatatgg aaatccaacg ttcaagagta aagggatatt
     1381 tatcccggag cgtgtgaaca taaacactgg cgagtcaggc ttggatattg ctcaaattca
     1441 ctctgggaaa tatatcggtc gtggcgttgc aatgtgcttt tgcccgttct gtggtgaaag
     1501 tttgaaaacg tgggaggaaa cgagtgagca atacaaaaaa tcatggcatg aaatttaaag
     1561 gaacaccagg tccatggcat gtaagtttcc acggttcagc taattgctgg gtagtggata
     1621 gtgaacgcga taaggcaatt gctaaggtga cagaatacaa caatgactgg cagatgcaga
     1681 aagctaattt tcagctttta gcggcagcac cagaattact tgaagccctg caagcggtgg
     1741 ttcgtgttgc tgacagaaaa accgatgaat tcgaaatggc ccattctgca attaacaaag
     1801 cattaggagt gatactgatg aacgtaatta aagaaatacc agtaatgcgt gatgaatatg
     1861 gctgctggac
//
LOCUS       DAZFYC010000311         1853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFYC010000311 DAZFYC010000000
VERSION     DAZFYC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.317"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..386
                     /gene="bssS"
                     /locus_tag="ACHKW5_005452"
     CDS             132..386
                     /gene="bssS"
                     /locus_tag="ACHKW5_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO7514594.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            501..1619
                     /gene="solA"
                     /locus_tag="ACHKW5_005453"
     CDS             501..1619
                     /gene="solA"
                     /locus_tag="ACHKW5_005453"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO7514595.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1667..1780
                     /locus_tag="ACHKW5_005454"
     CDS             1667..1780
                     /locus_tag="ACHKW5_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO7514596.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          479 a          444 c          442 g          488 t
ORIGIN      
        1 agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact
       61 gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt
      121 agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg
      181 acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa
      241 ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg
      301 ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc
      361 cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac
      421 cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc
      481 atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag
      541 gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc
      601 atatgccacc gcatcaacac ggcagccacc atggcgatac gcgattaatt cgccatgctt
      661 atggtgaagg cgaaaagtat gtcccgctgg tcctccgcgc gcaaacgctg tgggatgaac
      721 tttcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc
      781 cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg
      841 ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca
      901 actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa
      961 cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg
     1021 caattcgtca tgacgatgat ggcgtaacta ttgaaaccgt tgacggtgag tatcaggcga
     1081 aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc
     1141 agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata
     1201 aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag
     1261 aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg
     1321 aacgtgttcc gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca
     1381 atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc
     1441 ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg ctcattaccg
     1501 gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg
     1561 cgcaagacaa aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat
     1621 catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt
     1681 gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc tctggggatt
     1741 gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac tgacataaac
     1801 tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat tat
//
LOCUS       DAZFYC010000312         1835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFYC010000312 DAZFYC010000000
VERSION     DAZFYC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.318"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..915
                     /gene="espB"
                     /locus_tag="ACHKW5_005455"
     CDS             <1..915
                     /gene="espB"
                     /locus_tag="ACHKW5_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO7514597.1"
                     /translation="MTVNSVSENTTGSNAITASAINSSLLTDGKVDVSKLMLEIQKLL
                     GKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGAISSVLGILGS
                     FAAINSATKGASDIAQQTASTSSKAIDAASDTATKTLTKATESVADAVEDASSVMQQA
                     MTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAAVDKITGSTPF
                     IAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQEVRRAQDDIA
                     SRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            922..1329
                     /gene="cesD2"
                     /locus_tag="ACHKW5_005456"
     CDS             922..1329
                     /gene="cesD2"
                     /locus_tag="ACHKW5_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO7514598.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            1366..1587
                     /gene="escF"
                     /locus_tag="ACHKW5_005457"
     CDS             1366..1587
                     /gene="escF"
                     /locus_tag="ACHKW5_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO7514599.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            1593..>1835
                     /locus_tag="ACHKW5_005458"
     CDS             1593..>1835
                     /locus_tag="ACHKW5_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO7514600.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCSLV"
BASE COUNT          539 a          351 c          429 g          516 t
ORIGIN      
        1 atgacggtta attctgtttc ggagaatact accggctcta atgcaattac cgcatctgct
       61 attaattcat ctttgcttac cgatggtaag gtcgatgttt ctaaactgat gctggaaatt
      121 caaaaactcc tgggcaagat ggtgcgtata ttgcaggatt accaacagca acagttgtcg
      181 cagagctatc agatccaact ggccgttttt gagagccaga ataaagccat tgatgaaaaa
      241 aaggccgctg caacagccgc tctggttggt ggggctattt catcagtatt ggggatctta
      301 ggctcttttg cagcaattaa cagtgctacg aaaggcgcga gtgatattgc tcaacaaacc
      361 gcctctacat cttctaaggc tattgatgcg gcttctgata ctgcgactaa aacgttgact
      421 aaggcaacgg aaagcgttgc tgatgctgtt gaagatgcat ccagcgtgat gcagcaagcg
      481 atgactacag caacgagagc ggccagccgt acatctgacg ttgcagatga cattgccgat
      541 tctgctcaga gagcttctca gctggctgaa aacgctgcag atgccgctca gaaggcaagt
      601 cgggcaagcc gctttatggc tgcagtagat aagattactg gctctacacc atttattgcc
      661 gttaccagtc ttgccgaagg cacgaagaca ttgccaacaa cggtatctga atcagtcaaa
      721 tctaaccatg agattagcga acagcgttat aagtctgtgg agaacttcca gcagggtaat
      781 ttggatctgt ataagcaaga agttcgcaga gcgcaggatg atatcgctag ccgtctgcgt
      841 gatatgacaa cagccgctcg cgatctcact gatcttcaga atcgtatggg tcaatcggtt
      901 cgcttagctg ggtaattgat catggtcgat acgtttaatg atgaagtgct taatcactat
      961 cttgaacaaa aagggtacac aatacagaag gagtttcttt gtggcagtgc cttttttatc
     1021 ggatggcgga ttgagacctc ttttttctca ttagcgtaca gacttgatga acaagaactg
     1081 attttgtgct cttttgaagc acgtaaccaa acagggctta acggccctgt tttatcactg
     1141 actcgcttgc tcgaagagtt gtaccaccat ttttcgggta ttaagaaaat cagtgcgatg
     1201 aaatctaaga ttggttcaga ttcagaacgt caaaagcgcg aagagttgtt taattacttc
     1261 atcagaaagg gcgctgtgca gcaagaaaca gaagacggaa tttggttcgt aatgaatgta
     1321 aatagttaga aatttttatt tctattaact attgaggaaa atttaatgaa tttatctgaa
     1381 attactcaac aaatgggtga agtaggtaaa acgctgagtg attctgtgcc agagttactt
     1441 aatagcaccg atttggttaa tgaccctgaa aaaatgttag agctgcagtt cgcggttcag
     1501 caatattctg cttatgttaa cgtagaaagt ggaatgctga aaacgataaa agatttggtc
     1561 tcaaccattt ccaaccgtag cttttaattt ttatggttaa tgatgtttct gccaacaaga
     1621 tactggtgtg ggcggctgta gctgcggcaa atcataagct tcccaaatat gcagaatcta
     1681 tcctgaatgt attgccgcaa attatacccg ataaaaaaga tatcgcacat ttagaattta
     1741 ttattttata tggattaaat agaaaaaatg atgcggtaaa ggctctggag gactttatgg
     1801 atgatgagac aagccagttg ttatgcagtc tggtc
//
LOCUS       DAZFYC010000313         1807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFYC010000313 DAZFYC010000000
VERSION     DAZFYC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.319"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1807
                     /gene="rhsB"
                     /locus_tag="ACHKW5_005459"
     CDS             <1..>1807
                     /gene="rhsB"
                     /locus_tag="ACHKW5_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO7514601.1"
                     /translation="GKQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNP
                     AGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRL
                     KAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKY
                     DEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSFTDCSG
                     YVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTV
                     IAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDR
                     LIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWRYD
                     ERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQG
                     LANRCIPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAY
                     TPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTSVHTTAANL
                     DIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIAR"
BASE COUNT          436 a          562 c          541 g          268 t
ORIGIN      
        1 gtggcaaaca ggtgcgcagc tttacttacg atgataaata ccggggccgg atggtggcgc
       61 accgtcacac gggccggccg gaaatccgtt accgttacga cagcgacggg cgggtgacag
      121 aacagcttaa cccggcaggc ttaagctaca cgtatcagta tgagaaagac cacatcacca
      181 tcaccgacag cctggaccgc cgtgaagtgc tgcacacgca gggcgaagcc gggctgaagc
      241 gggtggtgaa aaaggaacac gcggacggca gcgtcacgca gagtcagttt gatgcggtgg
      301 gcaggcttaa ggcacagacg gatgccgcag gccggacaac agagtacagc ccggatgtgg
      361 tgacgggcct catcacgcgc atcaccacgc cggatggcag ggcatcggcg ttttactata
      421 accaccacag ccagttaacg tcagccaccg ggccagacgg gctggaaatg cgccgcaaat
      481 atgatgagta cggacgtctg attcaggaaa ccgcccctga cggcgatatc acccgctacc
      541 gttatgataa tccacacagt gacctcccct gcgcaacgga cgatgccacc ggcagccgga
      601 aaaccatgac gtggagccgt tacggtcagt tgctgagctt cactgactgt tccggttatg
      661 taacccgcta tgaccatgac cgttttggtc aggtgacggc ggtgcaccgc gaggaagggc
      721 tgagtcagta ccgcgcatac gacagccgtg gacagttaat tgccgtgaaa gacacgcagg
      781 gccatgaaac gcggtatgaa tacaacgccg ccggtgacct gaccaccgtc attgccccgg
      841 acggcagcag aaacgggaca cagtacgatg cgtggggaaa agccatctgt accacgcagg
      901 gcggtctgac gcgcagtatg gaatacgatg ctgccggacg ggtcatccgc ctgaccagtg
      961 aaaacggcag ccacaccacc ttccgttacg atgtactcga ccggctgata caggaaaccg
     1021 gctttgacgg ccgcacacag cgttatcacc acgacctgac cggcaaactt atccgcagcg
     1081 aggatgaggg gctggtcacc cactggcact atgacgaagc agaccgcctc acgcaccgca
     1141 ccgtgaaggg tgaaaccgca gagcggtggc ggtacgacga acgcggctgg ctgacagaca
     1201 tcagccatat cagcgaaggg caccgggtga cggtgcatta cgggtatgat gagaaaggcc
     1261 gcctgaccgg tgagcgtcag acggtgcatc acccgcagac ggaagcactg ctctggcagc
     1321 atgagaccag acatgcgtac aacgcgcagg ggctggcgaa ccgctgtata ccggacagcc
     1381 tgcccgccgt ggaatggctg acctacggca gcggctggct gtcaggcatg aaactcggcg
     1441 acacaccgct ggtggagtac acccgcgacc gcctgcaccg ggaaacgctg cgcagcttcg
     1501 gccgttatga actcaccacc gcttacaccc ctgccgggca gttacagagc cagcacctga
     1561 acagcctgct gtctgaccgc gattacacct ggaacgacaa cggcgaactc atccgcatca
     1621 gcagcccgcg ccagacccgg agttacagct acagcaccac cggcaggctg accagcgttc
     1681 acaccaccgc agcgaatctg gatatccgca tcccgtatgc cacagacccg gcaggtaacc
     1741 gcctgcccga cccggagctg cacccggaca gcaccctcag catgtggccg gataaccgta
     1801 tcgcccg
//
LOCUS       DAZFYC010000314         1807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFYC010000314 DAZFYC010000000
VERSION     DAZFYC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.320"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(440..1417)
                     /locus_tag="ACHKW5_005460"
                     /pseudo
     CDS             complement(440..1417)
                     /locus_tag="ACHKW5_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          454 a          414 c          398 g          541 t
ORIGIN      
        1 taatgtgata aaaaaaatat ttatctcgat gctacccaac atacgactca tttatttttc
       61 tcggtttctc cttgtatata tatgttgctt tataatttta ctacgcacgt caggcgtatc
      121 tgtaacatag ctccgcatta tgcaatgcga ctttgcatac aaataggaat aaatctaata
      181 gctgtagatt tgctcctgta tttcctgaac caagcgagcc agtacatgat gcatatgtct
      241 ggaaaataat tcagagtaag gataagtcca gctttgttga ataaatcagg tttgggataa
      301 gccccctcag ttggtagcta ctgcaggtaa tacgcacgta ttatgacatt cgtgccctcg
      361 tcattttgtt tagtacacgc actgtagtgg cacagaactt ctgcgacctg accatcctaa
      421 atagctgcgc cgaataccgc tacactttta acagcccata tttaccaccg gggaatgggc
      481 tcacggtttc cataaaataa tgaacctttt taagcagtta ccacattgaa cggcactgat
      541 gattgcgcgt tatcgtatcg tgaagcgcct gctataaccg ctcaacatga ttcacccacg
      601 gcgagtaaac tggctgataa atcactctga acttcggatt ttctttcagc cagcgctgtg
      661 tttcccgact tttatggatg atgtagttgt ccactatgag cgtgataggt ttatcccgtc
      721 ggtatgtcaa tttcagatgt ttcaacagac tgataaataa ggctgagctt ttgctgttgc
      781 cacccacata gctgactttg cccgttccac tatgtaaagc accagccaga tagtattttt
      841 cattctgccc cggtgttacc acatgttttt gctgtctgcg aagttgccag tccgcaccgg
      901 ttttgggatt aagatagata tacacttcat cttcataaaa gaccggatgc tccgcactac
      961 attcgtccag tgctttgtga attgctgctt tatgcgggtt acgggtacgc agggttggcg
     1021 cagccctgcg ccacacaagc ccgactaatg gcaaccagcg gcgaacggtt ccggcatgta
     1081 actggcactc ggttatctcg ttgattttta ttgccagcag ttctgtactc cagcgtgaac
     1141 gctgatagcc aaaatatccg ggagagtgtt ttatcagttc acgaaacaat gtgcagatat
     1201 gctcaaacgg ccagcggcgg gaacgtcctg cgggtaatga tttcagacct tcaacactcg
     1261 acaacgtgaa ccagttaatc cagcgcctga cggaggaacg ggcgcagcag agcgttctgg
     1321 caacgtcact gacgcgagcg ctccggtgca gcatcagcat ggcggtcagc ctgcgggcat
     1381 aatttttatc gtgagtttta tagattgctt tttgcatcag gcgtcattcg tcgcggggaa
     1441 ttggtgctat gatcggcatc gctcagtccg gttagggggg gatttttggc gattgatcag
     1501 atcgcacaat ccgggctgag ttccctttca ttgatctact attcggcgca gctatttagt
     1561 tggtgatttt gaacttctgc tttgctacta agcagcctgc gttttcagga aaatacgtga
     1621 tgtgatcctg caactcagca acagttcggt tgattcaaca aagcgttttt taacaataat
     1681 tcttcattgt aaaaagcatc ttcagttgtg atcaaatatt attattctta ttggtgttct
     1741 catataatga aatagaatta attttagccc ccctacacaa gtggctgagc ttctctactc
     1801 aatttta
//
LOCUS       DAZFYC010000315         1784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFYC010000315 DAZFYC010000000
VERSION     DAZFYC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.321"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1713)
                     /gene="nleL"
                     /locus_tag="ACHKW5_005461"
     CDS             complement(<1..1713)
                     /gene="nleL"
                     /locus_tag="ACHKW5_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO7514602.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMEN
                     GSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVT
                     QQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWM
                     SFFLY"
BASE COUNT          603 a          366 c          271 g          544 t
ORIGIN      
        1 atataaaaaa aaggacatcc agttagttga tgtattagga gataacatat caaccatatg
       61 actcaaagaa cacatcattg ccataccatc atcctgtgat gataacaaaa cccagttata
      121 agccaaatcg gaatcatcat cccagtcagg cttcccagag ccatcgcaca atccaaaatc
      181 actctgctgt gtgacaggat aaactttttc attttttaaa tatacttcat agagttccct
      241 aaatcgttcc tgcattccat cacgccgtga accattcatt acaatttgta taaaggaact
      301 attataagat accatcgcac ctggatgtcg ctcaaaatac atccctgcct gcaataaact
      361 accattctcc atatacatca ttattgaatt attaaaccaa gacattatag tatcaaaaaa
      421 gtcatcacat ataggctcga gccattgctg tatatttgcg ttgttcaaat aaggatgaat
      481 actaaatgaa tcagccaaag acatcttaac atagggccgg aacgccgcta attcgtcaag
      541 agatattttt ttgataagtt cttcagcggc ccttacttta tcatggttat acttatcatc
      601 agatgtattc atagtcaata aaattgattt atttgatttt gtatagttat ccaacacaga
      661 taatatattt tcctgtatgt cagatagatc tatgtaacac cctcctagat taatggtatt
      721 acttttttca ctaaagagct cctttacatc acctaagata gcaccactta aaaccattcc
      781 aggaattata atagctcctg ttaaatcagc ctcgttcaaa attggaggat attgtatata
      841 tgaagatcct ttaaatgatg catttttaag attggaattg ctgaaattag agttttcaaa
      901 caaagcccta ataatacaat tagataaatt agcataacaa aaattacatt cattcaaaat
      961 cgagttttta aaagaacagt tttccaatac agcctgagac aaatcactca tcctgagatc
     1021 tgtatcatca agcactgctc cagaaaaatt tactgatgat aaattcagcc ctacaagact
     1081 tagacctgac aagtcacatc cagagtaatt caattcttca gcagactctt cacccgttcg
     1141 gttagccgac aaccaaatta agtcagcagt aagctcagct ttgctcaggc aggcacggcc
     1201 ttgactgaca tcatatgcaa ggaattgaca ttgcgattca tttaatgaat tacagccatt
     1261 taaatatacg gaatttctga gtgcctcagg aaaggaacct tctatatgtt tcaaagaact
     1321 acaatggcat aaagataaat taatgacatt ttcaggtatg ctgcaattta taacctctaa
     1381 tgatgagcat ccaaccatac ttaatgaaga taagttgggg ggtaagcagt ttattgattt
     1441 aagctctgta catccattca aaaccagttc tttcagagaa cctggaagca agtctggtaa
     1501 tgttgttatt ggctcactga ttgataaagt ctctccatta gtacttataa catcaagtat
     1561 ttttgatgca acctcatgac gattttcgct aaattctccc tcagcgcacc acttttcgag
     1621 ggcaacttca acatcctcgt tagatggtga atctacagga ctttcaaaag atattactcc
     1681 agaatttaca gagatatttg tagtgggcag catttatctc actccttttt acttagtcca
     1741 ctaaagtaaa attagaaaac acaactgccg ggacaacttt ctgt
//
LOCUS       DAZFYC010000316         1783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFYC010000316 DAZFYC010000000
VERSION     DAZFYC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.322"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..646)
                     /locus_tag="ACHKW5_005462"
                     /pseudo
     CDS             complement(1..646)
                     /locus_tag="ACHKW5_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
     gene            complement(810..920)
                     /locus_tag="ACHKW5_005463"
                     /pseudo
     CDS             complement(810..920)
                     /locus_tag="ACHKW5_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(967..1254)
                     /gene="nleC"
                     /locus_tag="ACHKW5_005464"
                     /pseudo
     CDS             complement(967..1254)
                     /gene="nleC"
                     /locus_tag="ACHKW5_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
     gene            complement(1288..>1783)
                     /gene="nleB2"
                     /locus_tag="ACHKW5_005465"
     CDS             complement(1288..>1783)
                     /gene="nleB2"
                     /locus_tag="ACHKW5_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO7514603.1"
                     /translation="SKEQDSLISMYAKKRNHAWFDFFRNLALLKAGEIFRCTYNTKNH
                     GISFGEGCIYLDMDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNRSNHPAL
                     LEGLSFMHSKVDAHPYYDGLGKGVKKYFNFTPLHNYNHFCDFIEFKHPNMIMNTSQYT
                     CSSW"
BASE COUNT          553 a          401 c          287 g          542 t
ORIGIN      
        1 atttatttta tccattctaa taccaataat atctctatta tcgccatata ttttctctgc
       61 actcccggca ccataatact ggttgaaaca acgaacttcg cttgtcacct cttcatggct
      121 ttgagatata gtaaacatct tcaacacttt tgttgtattt tccatatctt catacacgac
      181 agcattacca cctttaccaa taacgttacc tagcacgggt gatttgttgt tgctaagcct
      241 cagcaacgct gactgagtat aatccgctgg cggtaactcc ggctgcgcat aatccactgg
      301 aggtaaatca ggtctgttcg aacgaacaga tcctctttct atagtcgtgc tcactgtcga
      361 tgaattcagc atagcctcaa tttttctggt gatctctccc ttaggccatc ccagtctata
      421 cagcatatta gtaaaacatc cactatgact ctctcttgtc acgcaaaacc tattatcact
      481 taaaacaacg cgatatgttc tgttgctaac ctttacttgc gccccattat cagagtgagc
      541 ggcagcatcc cttacagagg gataaaacac gattatcagg caaagtcagg tttctggtta
      601 aagaattcca tgaacacccc aaatttacag aagatggtga tagcatacat ttcaaccttc
      661 aaaatgaatc aatctttact ttcttaacaa acatcaccat gacatgacaa caaaaaactg
      721 gaaattaact attgtttttg agtatctggc aactcattca tccttccagc atcccgtcag
      781 acgaacgttt acgaactata aatcaggtat tactggccct cgaaatctgg agatactgac
      841 gtatcaacac ggttcagtaa cacccgatac ctcttccaga ctgtcaacaa cgatgtctct
      901 tcatccgttg cgatctgcag catcctgaat atgaatacca tagcttacgg catcacgaaa
      961 agtttatgag cggctcaaag catcaagtat agtattatta atcatatcga ccgtatgctg
     1021 atcaatgagt ctgccacgac tgtcatggac ggcacgaatt acactttcgt atacgttgct
     1081 taaatctgct gcggaaagtg gtattcgctt gcctatatcc aaaacgtaat ccgcataggc
     1141 attttcagca cgattaggag caatgagagc agagtatcct gccggggcgg aaaagttgaa
     1201 gttggactgt aatgagggaa ttttcatgtt gatacctaat accatgcaaa tcatttatta
     1261 caacagaata aactttatca aattcattta ccatgaactg catgtatact gacttgtgtt
     1321 catgatcata ttagggtgtt taaactcaat aaagtcacaa aaatgattat aattatgtaa
     1381 tggtgtaaag ttaaaatatt tcttaactcc tttccccaaa ccatcataat atggatgagc
     1441 atctacttta ctatgcataa aagaaagtcc ctcaagtaga gcaggatgat tactacggtt
     1501 aacaattatt gcactatttt caatatttac actatcatta cgacgatcca catgcattga
     1561 aattccatca ggagcatata ttgtaccaag cttacctgta agtatcatat ccatatcaag
     1621 atagatacac ccctccccga atgaaatacc gtgattcttt gtattatatg tgcacctgaa
     1681 tatctctcct gcttttaata aggctaaatt tctgaagaaa tcaaaccatg catgatttct
     1741 tttctttgca tacatagaaa tcaaagaatc ctgctccttt gaa
//
LOCUS       DAZFYC010000317         1782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFYC010000317 DAZFYC010000000
VERSION     DAZFYC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.323"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..508
                     /locus_tag="ACHKW5_005466"
     CDS             53..508
                     /locus_tag="ACHKW5_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO7514604.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFME
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            609..817
                     /locus_tag="ACHKW5_005467"
                     /pseudo
     CDS             609..817
                     /locus_tag="ACHKW5_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            918..1739
                     /locus_tag="ACHKW5_005468"
     CDS             918..1739
                     /locus_tag="ACHKW5_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7514605.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT          457 a          463 c          472 g          390 t
ORIGIN      
        1 tttatcacct gatttgtcag tgtcatcgtc atgactcaga gaggtaaata ccatgattca
       61 cctgttcaaa acctgcatga ttaccgcctt cattctgggg ttaacgtggt ctgccccact
      121 ccgggcacag gatcaacgtt acatcagtat acgcaataca gatacgatat ggctcccggg
      181 aaatatttgt gcctaccagt tccggctgga taatggcgga aacgatgaag gatttggccc
      241 cctcaccatc actctgcaac tcaaagacaa atatggtcag acgctggtga ccagaaaaat
      301 ggaaacggaa gcctttggtg acagtaatgc cacgcgaacc acagacgcat ttatggaaac
      361 ggagtgcgtg gaaaatgtcg ccacaaccga aatcattaaa gcaactgaag aaagtaacgg
      421 ccatcgtgtc agtctgccgt tatcggtttt cgatccccag gactaccatc cactgctgat
      481 taccgtttcc ggaaaaaatg ttaactgatc cctgaagtca gtttcatgct tcagcctctg
      541 ccacaacgta tggcagaggc tatttatcag gagaaaccag atatgccggg ttacacacag
      601 agtatgtcct ggctgaagga tctttctcat acgggcagac ggaggctgtc acaacagctt
      661 accttaatgt gcttattgaa gatgaccagg tgcgcacttc cggctggtta tccgcaacgc
      721 ggaaggtcag cgacgctggc gctgccggaa tactgaaccc tgtgccggaa agcagcttaa
      781 tgcgtggctc gccagtgaag ggcttctcag acaataaacg tcattgattt cgtccatcag
      841 gccgcgtctt ctccgggaga cgcggctttt ttcatttatt acaccagtaa atcttaacca
      901 ccgataagga gcaaagtatg cgattagcca gtcgttttgg ttatgctgca aaccagatac
      961 gccgtgaccg tccactgaca catgaagagc tgatgcacca tgtgccgggg attttcgggg
     1021 aagataaaca cacgtcccga agccagaact atacgtacat ccccaccatc accgtgctgg
     1081 aaagtctgca gcgggaaggc ttccagccat tcttcgcctg ccagacccgt gtgcgcgacc
     1141 cgggccgtcg gggatacaca aaacacatgc tgcgtctgcg gcgggccgga gagataaacg
     1201 gagaacatgt ccctgaaatt attctgctca actctcatga cggtacctcc agctaccaga
     1261 tgctgccggg ttacttcagg ttcgtctgcc agaacgggtg cgtctgtggt cagtctctgg
     1321 gggaagtgcg tgttccgcac cggggaaatg tagtggagaa agttatcgaa ggggcttacg
     1381 aggtggtggg ggtttttgac cggatagagg agaagcgtga tgccatgcag tcgctggtcc
     1441 tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt tatggtgacg
     1501 aacatcagcc cgtcaccacc gccgacattc tgacgccacg acgccgggag gattacggaa
     1561 aggacctgtg gagtgcttat cagaccatcc aggagaatat gctgaaaggg gggatttccg
     1621 gtcgcagtgc cagaggaaaa cgtatccaca cccgtgccat tcacagcatc gacaccgata
     1681 ttaagctcaa ccgcgcattg tgggtaatgg cagaaacgct gctggagagt atgcgctgat
     1741 gaaacatatc cctgaaggca tgattcaggt accagaatca cc
//
LOCUS       DAZFYC010000318         1767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFYC010000318 DAZFYC010000000
VERSION     DAZFYC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.324"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..150)
                     /locus_tag="ACHKW5_005469"
     CDS             complement(<1..150)
                     /locus_tag="ACHKW5_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514606.1"
                     /translation="MAIKGLDQTIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKV"
     gene            complement(165..518)
                     /locus_tag="ACHKW5_005470"
     CDS             complement(165..518)
                     /locus_tag="ACHKW5_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7514607.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(511..885)
                     /gene="gpFI"
                     /locus_tag="ACHKW5_005471"
     CDS             complement(511..885)
                     /gene="gpFI"
                     /locus_tag="ACHKW5_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7514608.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEIQS
                     SGEDVQVGDTVIRERETAAHDVREDTSGALTRIRVLTCLHLCGIDGETGESVEIADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(937..>1767)
                     /locus_tag="ACHKW5_005472"
     CDS             complement(937..>1767)
                     /locus_tag="ACHKW5_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7514609.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEIQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNITQGSGK
                     EWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPEGMLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
BASE COUNT          415 a          561 c          389 g          402 t
ORIGIN      
        1 aacctttgtc tcccgggcaa cctgtgatga agactgatta atcgccgttg tggccacgcg
       61 gttaatggcc attgctgaag ccgccggaat ggcgttttta cgaacccggc tcagattttc
      121 aatcgtctga tcaagccctt ttatcgccat aattcaccct gcgtttatcg tcgccggtta
      181 acggcgggtg gttgaccacg gttgagccag agataacagc tgcccccgtc atccggagaa
      241 acacgatcca cccagaatat ctcaccatta atggtcagcg tgtcaccacg ccgcacggca
      301 cgaaccgtat ccgtccgcac aaataatgac gggctgcttc cttcaatacg gaccccgcca
      361 ccggcaaacc ccagcgactc cggatcgtca aaaaccccct gaacttcgct gccacactgt
      421 gcccccgagg tgaactgcgc acagagcccc atcacttcaa caatcgtgct gtccaccccg
      481 gcaagggcag catcaaaggc attctgaaaa tcacgcataa acagccattc cgccatcaac
      541 gtgtgttttt gcatctgagg acataatcag aatcacccga ccaacatccg caatctcaac
      601 ggattcccct gtttcaccat caatgccaca gagatggagg caggtcagaa ctctgatgcg
      661 cgttaacgca ccggatgtat cctcacgaac atcatgagcc gcggtttccc gctcccggat
      721 caccgtatcc ccaacctgaa catcctcgcc ggatgactgt atttcctctt cccattccgc
      781 cacccgctgc gctatctctg cggcactccc ggatatatcc ggctcacgcc ccagaatcag
      841 ggccagttca tcaagccgtt tcagattttg ctctttcgtt gccatatcag ccccctgtga
      901 aaaaagacac gggggcattt cgcccccgct cacggattat ttcacctgta ccaccacaaa
      961 ctcatccggg tccggcaaca ccatcagcgg cgcggactgc gtcatggtaa attcacgggc
     1021 gggatccccc accgtcagcc agtgtttcgg ataacgggaa gaggccacca caccttcgga
     1081 caacgcctgc gcatcctgaa tggcaccgta acaacggatc ccatctgcag cagtattccc
     1141 cagaaccagc atgccctccg gcagataacg tttttcgata ccgtcttctg ctatataaga
     1201 cgttttcgcc accacaatgg ccagatcgcc gtaatacccc ttgaaggaca ccactgcgcc
     1261 cagatctttc actgccgttt cgagttgaga atttgaaccg cgacgggtat ccagtttttc
     1321 gcggaacagc ttaaaaccat tcagaagacg ccagacggta ccgtccataa tggcaatatt
     1381 cacaagaccg ctggcctggt cgcagtagag gtcaagatca tgtgtaggat cgaacgtgtc
     1441 acgatcctgt tttgaccact ccttaccact accctgagtg atgttattct tcgtcgacct
     1501 gccaaaatcg acctcaattt tctcgaactg gtctccttcc atcgtatatt tgccatacaa
     1561 cacagcattt accgcctgta tttcttccac ctggacaatc gcgtgctctt cctgtttgag
     1621 gttatcggta atgatacgca gacggcggta agccggatcg ttcagttgag ccggatcttc
     1681 accaggaagg cgctcaaccg cctgctggta attaaattcg tgtttcggct tgacgtagcc
     1741 cggacgtaac acgcgggttt caccacc
//
LOCUS       DAZFYC010000319         1764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFYC010000319 DAZFYC010000000
VERSION     DAZFYC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.325"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1764
                     /locus_tag="ACHKW5_005473"
     rRNA            <1..>1764
                     /locus_tag="ACHKW5_005473"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          478 a          384 c          552 g          350 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac
      181 tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag
      241 gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa
      301 cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc
      361 gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg
      421 ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg
      481 ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga
      541 ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg
      601 gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg
      661 gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat
      721 agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag
      781 ctgcggcagc gacgctgatg cgttgttggg taggggagcg ttctgtaagc ctgtgaaggt
      841 gtgctgtgag gcatgctgga ggtatcagaa gtgcgaatgc tgacataagt aacgataaag
      901 cgggtgaaaa gcccgctcgc cggaagacca agggttcctg tccaacgtta atcggggcag
      961 ggtgagtcga cccctaaggc gaggccgaaa ggcgtagtcg atgggaaaca ggttaatatt
     1021 cctgtacttg gtgttactgc gaagggggga cggagaaggc tatgttggcc gggcgacggt
     1081 tgtcccggtt taagcgtgta ggctggtttt ccaggcaaat ccggaaaatc aaggctgagg
     1141 cgtgatgacg aggcactacg gtgctgaagc aacaaatgcc ctgcttccag gaaaagcctc
     1201 taagcatcag gtaacatcaa atcgtacccc aaaccgacac aggtggtcag gtagagaata
     1261 ccaaggcgct tgagagaact cgggtgaagg aactaggcaa aatggtgccg taacttcggg
     1321 agaaggcacg ctgatatgta ggtgaagtcc ctcgcggatg gagctgaaat cagtcgaaga
     1381 taccagctgg ctgcaactgt ttattaaaaa cacagcactg tgcaaacacg aaagtggacg
     1441 tatacggtgt gacgcctgcc cggtgccgga aggttaattg atggggtcag cgcaagcgaa
     1501 gctcttgatc gaagccccgg taaacggcgg ccgtaactat aacggtccta aggtagcgaa
     1561 attccttgtc gggtaagttc cgacctgcac gaatggcgta atgatggcca ggctgtctcc
     1621 acccgagact cagtgaaatt gaactcgctg tgaagatgca gtgtacccgc ggcaagacgg
     1681 aaagaccccg tgaaccttta ctatagcttg acactgaaca ttgagccttg atgtgtagga
     1741 taggtgggag gctttgaagt gtgg
//
LOCUS       DAZFYC010000320         1734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFYC010000320 DAZFYC010000000
VERSION     DAZFYC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.326"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(185..352)
                     /locus_tag="ACHKW5_005474"
     CDS             complement(185..352)
                     /locus_tag="ACHKW5_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7514610.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(349..777)
                     /locus_tag="ACHKW5_005475"
                     /pseudo
     CDS             complement(349..777)
                     /locus_tag="ACHKW5_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            complement(958..1479)
                     /locus_tag="ACHKW5_005476"
     CDS             complement(958..1479)
                     /locus_tag="ACHKW5_005476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514611.1"
                     /translation="MFIFQSLPEVRSVITYSDIQHAYDEMRRHNARVHDSLRREVKLL
                     KKCYACSLSGESSALDMERLVRIERMTESGRYEECSPSCLGMNKDGVIEFRICTLINE
                     TPTGNMFVPAYITMEYCDDLIKIGMSLSNFRSDKRAELYVSKGEEAERYVEASEYIKS
                     MMIRFIADQKFSG"
     gene            complement(1561..1637)
                     /locus_tag="ACHKW5_005477"
     tRNA            complement(1561..1637)
                     /locus_tag="ACHKW5_005477"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Arg,seq:tct)
     gene            complement(1651..1727)
                     /locus_tag="ACHKW5_005478"
     tRNA            complement(1651..1727)
                     /locus_tag="ACHKW5_005478"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1691..1693),aa:Arg,seq:tcg)
BASE COUNT          460 a          502 c          349 g          423 t
ORIGIN      
        1 cccctccgga gaggggcatg tttgcatgca cattcttttt cttgcatggt gccgggtgcc
       61 tcccggtgaa ttcagtatca gcacctgaat ccgcgattat cccatatacc tggttgctga
      121 tcgcccctcc gcacaggggg attcaccatg cagaagtgtt tttaataaac agcaaacaaa
      181 aaaatcaagc attatgcagg ctgtttcttt ttatcaccgg ccacagcaat accacaatgc
      241 cgcagaccag caccccatcc gccagcaccg acatgattct gctggtgaaa tccaccatca
      301 ccaccagaaa cagcaggagt gcagccacag ctaggcgcag ttttaccgtc acaggtgatt
      361 ctccagacga agacccagaa caccggcaat ctcttccagc accttgcgct cttccggctc
      421 aatttcgccg tctgcctccg caatggccac cgccacatcc agcacatctt ccgcttcacg
      481 cgtatcgtgt ttcacatcct caatctcgcg taacgcggca cgacgaccaa ttttaaaatt
      541 ggtatccagc tgaccgataa tagttgcgct aatcgcatta atttccgagg taaacgcgta
      601 cagcgcaggc tgattacgca gtacctgttc gatcttcgct ttctaggaag cctcacattc
      661 accatctgca taggccacca ggtatgcggc gttaatcacc acctgtgcca gatcgcgttt
      721 ttcaaacttt ttaatttccg ttgccgctct gcgggctttt tttaccaaaa ataccaaaca
      781 tcgtgacgtt cctttgggtg ggtgagccaa cgcccgggag cgatctgccc acagagaaag
      841 tcacactgac cactccgtaa gctcaccccc gaaaggctct gtggttgata tgcgccgggc
      901 gtgacgcgga tacaaaaaag gcccgcaaaa gcgagccata acatcaaaaa ataactatta
      961 tcctgagaac ttctgatccg caatgaagcg aatcatcatg ctcttaatat attcagacgc
     1021 ttctacgtat cgttcagcct cttcaccttt ggaaacatat aactcagctc gtttatcact
     1081 tctgaagttg gaaagcgaca tccctatttt tataaggtca tcgcaatact ccatagttat
     1141 atatgctggc acaaacatat ttcccgtcgg agtttcatta attaatgtac atattctgaa
     1201 ctcaatcaca ccatccttat tcatcccaag acatgagggt gaacattcct cataccttcc
     1261 gctctcggtc atccgctcga tcctgaccaa tcgctccata tccagcgcag agctttcacc
     1321 actaagcgaa cacgcataac acttcttcag aagtttaact tcccgcctca gggaatcatg
     1381 aacacgcgcg ttatgccttc tcatctcatc atatgcatgt tgaatatcac tatacgtaat
     1441 caccgaacgg acctccggaa gcgactgaaa aataaacatt ctatacctcc ttccagaaag
     1501 agtcatgtcc tggtcacgac acaaaaaaat cccgcaaaag cgaacccggg aaaataagtg
     1561 tggcgcgttg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     1621 gactgagcta acaacgcatg atgctgataa tggaccgcca tcggggacct gaaccccgcg
     1681 cagccagctt cgaaggctgg cgctctgtcc cgatgagcta atggcggtat ctga
//
LOCUS       DAZFYC010000321         1704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFYC010000321 DAZFYC010000000
VERSION     DAZFYC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.327"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..241
                     /locus_tag="ACHKW5_005479"
     CDS             5..241
                     /locus_tag="ACHKW5_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO7514612.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
     gene            198..644
                     /locus_tag="ACHKW5_005480"
     CDS             198..644
                     /locus_tag="ACHKW5_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7514613.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEGWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKANMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            641..1168
                     /locus_tag="ACHKW5_005481"
     CDS             641..1168
                     /locus_tag="ACHKW5_005481"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7514614.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEVDDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1165..1347
                     /locus_tag="ACHKW5_005482"
     CDS             1165..1347
                     /locus_tag="ACHKW5_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7514615.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            1344..1514
                     /locus_tag="ACHKW5_005483"
     CDS             1344..1514
                     /locus_tag="ACHKW5_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO7514616.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEGDG"
     gene            1507..>1704
                     /locus_tag="ACHKW5_005484"
     CDS             1507..>1704
                     /locus_tag="ACHKW5_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7514617.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRNKE
                     REKAEKTAEKKRRREEQKQKDK"
BASE COUNT          503 a          388 c          483 g          330 t
ORIGIN      
        1 gagcatgagc gacctatcat taacccagcc aaagctaaaa gaatgtccgt tttgcggcgg
       61 taatgctcgt ctgtgggttg aggccggaat aaatattgat gtgtggggct atgcagaatg
      121 tgacctctgt gaagccaggg tggcatgggc accatcagtt gctgcggcta ctgaaaaatg
      181 gaaccggaga gcaggagatg aagcaaacct ttctgcttcg caacgaagca atcagaaata
      241 acgccataga cgccattctc tcactaccca tcgacgacaa gtcaccccac gaagtccacg
      301 ttaaagaacc caagcgcagc aaagcgcaga atgaccgcat gtggccgatg ctgaacgatg
      361 tttcgcgtca ggtgctatgg catggtcaac ggctggcgcc ggaaggctgg aaagacctgt
      421 tcactgccct gtggcttaag accaaaaaac tggagcaaag aagtgcgcct ggtatcgacg
      481 gtggcgttgt catgcttggc gtgcgtacca gcaaaatgcg aaaggccaac atgactgagc
      541 ttatcgaaat catgttctgg ttcggctcag agcgcaacgt gcggtggagt gatgactccc
      601 ggcgagagta tgaatggtca caacgaaaag gtagggctgc atgactatca aatcaaatac
      661 gccagcacac gacaaggact gctggcaaac gccgctttgg ctttttgatg cactggatat
      721 tgagtttgga ttctggctgg attcagctgc gagcgacaaa aacgctctgt gcgctcactg
      781 gctaactgag gtcgacgacg cgctcaattc tgagtgggta agccacggtg caatctggaa
      841 taacccaccg tacagcaata tcaggccgtg ggtggaaaaa gccgctgagc agtgcataca
      901 acagcgacag acggtagtga tgcttgtgcc agaggatatg tcagtcggat ggttcagcaa
      961 ggctctggag agtgtcgacg aagttcgcat catcactgat ggacggatta attttatcga
     1021 accatcgaca gggctggaga agaagggaaa cagcaaaggc tccatgctgc tgatttggcg
     1081 accgttcatc agtcctcgac gaatgtttac tactgtatcc aaagcggcat tgatggcgat
     1141 cgggcagggc gtcaggaggg cggcatgaga cgacagcgac gaagtttcac cgacatcatc
     1201 tgcgaaaact gcaaatacct tccaacgaaa cgctccagaa ataaacgcaa gccaatccca
     1261 aaagaatctg acgtaaaaac cttcaactac acggctcacc tgtgggatat ccggtggcta
     1321 agacattgtg cgaggaaaac aaggtgattg accaaaatcg aagttacgaa caagaaagcg
     1381 tcgagcgagc tttaacgtgc gctaactgcg gtcagaagct gcatgtgctg gaagttcacg
     1441 tgtgtgagca ctgctgcgca gagctgatga gcgattcgaa tagctcgatg cacgaggaag
     1501 aaggtgatgg ctaaaccagc gcgaagacga tgtaaaaacg atgaatgtcg ggaatggttt
     1561 caccctgcat tcgctaatca gtggtggtgc tctccagagt gtggaaccaa gatagcactc
     1621 gaacgacgaa ataaagaacg cgaaaaggcg gaaaaaacag cagagaagaa acgacgacga
     1681 gaggagcaga aacagaaaga taaa
//
LOCUS       DAZFYC010000322         1663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFYC010000322 DAZFYC010000000
VERSION     DAZFYC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.328"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..>1663
                     /gene="tir"
                     /locus_tag="ACHKW5_005485"
     CDS             52..>1663
                     /gene="tir"
                     /locus_tag="ACHKW5_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO7514618.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSDQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPTTTDPDTAASTAEAATKDRLT
                     QEAFQDPDKQKVNIDENGNAIPSGELIDDVVAQIAEQAKAAGEQARQEAIESNSQAQK
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARIPEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANASPTPGPARF"
BASE COUNT          462 a          368 c          436 g          397 t
ORIGIN      
        1 gcaatataaa ggattattat ctatttacat aaaataaaaa ggagacattt tatgcctatt
       61 ggtaatcttg gccacaatcc caatgtgaga gctttaattc cacctgcacc gccattacct
      121 tcacaaaccg acggtgcagg aggtgcccgt aatcagctca ttaactcaaa tggcccgatg
      181 gggtctcgtt tgctatttac gcctataagg aattctgttg ctgatgctgc tgattctcgt
      241 gccagtgata ttcccggact tcctacaaat ccactgcgct ttgctgcgtc cgaggtatct
      301 ttgcatggtg cgcttgaagt tcttcatgat aaaggggggc ttgatactct taactctgct
      361 attggatctt cgttattccg tgttgaaact cgggatgatg gcagccatgt tgctatcggg
      421 caaaaaaatg gcctcgagac cactgttgtt ttaagtgatc aagagttttc tagcttacag
      481 tcccttgatc ctgaaggtaa aaacaaattt gtatttactg gaggccgcgg tggcgcaggg
      541 catgctatgg tcacggttgc ttcagatatc gccgaagccc gtcagaggat aatagataaa
      601 ttagaaccaa aggatacaaa ggagacgaag gagccagggg atccaaatag tggcgaggga
      661 aaaatcattg aaattcatac ctcaacctca acttctagcc tccgtgcaga tcctaaactt
      721 tggttgtcat tggggactat tgctgcaggt ctgataggga tggctgcgac gggtattgca
      781 caggctgttg cgttgactcc agagccggat gatccaacta ctaccgatcc tgatactgca
      841 gcaagcacag ctgaagctgc gacaaaagat cggttaacgc aggaggcatt ccaggatcca
      901 gataaacaaa aagttaatat cgatgagaac ggaaatgcaa ttccgtcagg ggaattaata
      961 gatgatgttg ttgcgcaaat agcagaacaa gctaaagcgg cgggggaaca ggccagacag
     1021 gaagctattg aaagtaattc tcaggcgcag aaaaaatatg atgagcagca tgctaaacgc
     1081 gaacaggaaa tgtctctttc atcgggggtt ggctacggta ttagtggggc gctgattctt
     1141 ggcgggggaa ttggtgccgg tgttactgct gctcttcatc ggaaaaacca accggcagaa
     1201 caaacaatca ctacacgtac agtagtcgat aatcagccta cgaataacgc atctgcacag
     1261 ggcaatactg acacaagtgg gccagaagag tccccggcga gcaggcgcaa ttcgaatgcc
     1321 agccttgcat cgaacgggtc tgatacctcc agtacgggca cggtagagaa tccgtatgct
     1381 gacgttggaa tgcccagaaa tgattcactg gctcgtattc cagaggaacc tatttatgat
     1441 gaggtcgctg cagatcctaa ttatagcgtc attcaacatt tttcagggaa cagcccagtt
     1501 accggaaggt tagtgggaac cccagggcaa ggcatccaaa gtacttatgc gcttctggca
     1561 agcagcggcg gattgcgttt aggtatggga ggattaacgg gggggggcga gagcgcagta
     1621 agtactgcca atgcctcacc aacgccggga cccgcacgtt tcg
//
LOCUS       DAZFYC010000323         1636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFYC010000323 DAZFYC010000000
VERSION     DAZFYC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.329"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..563)
                     /locus_tag="ACHKW5_005486"
     CDS             complement(<1..563)
                     /locus_tag="ACHKW5_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514619.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLG"
     gene            682..1636
                     /locus_tag="ACHKW5_005487"
                     /pseudo
     CDS             682..1636
                     /locus_tag="ACHKW5_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          535 a          315 c          288 g          498 t
ORIGIN      
        1 ccaagttttg gaggtttgat aggcgtttta atttcactgc gcaacgcatc taagtttgta
       61 agatcactgt ctataatttt aagatagttt aaatctaacg aaccatcagt tttatcaagc
      121 ttagttttca gccttttaac agcctccaag gaagcatcaa gaactggtgt ctgattcttc
      181 aaagcttctg aaaaactttt ttgtgttgat tttcgagctt gttgttgagc agattttctt
      241 aactcttttt cacgcttaac ttgttctaaa agctctttac ggtcatcaga acgtgaacca
      301 aaaaatctat cagcaagttc agttaacaaa ttagatataa tagttttcag ttctcttgca
      361 gcctggtttc ttatgaatcc ctctctcccc gacttatctt tcagctcttt attactggat
      421 tgagtaattc caatataacc aaaaatcctt ctattagacc aataatatcg ccctgcattc
      481 catgaacgtc tttcttctat ctggaagaaa tcattatcta ctcgaccata aggtaatact
      541 ctcaagctat ccctaaaaat cattagtcct gcatactttt tggccttaag atcaaagtgg
      601 gaatgttcac gttcagtatg tgatgtattt tgtgaaagga attcaaacgt tcctatctga
      661 agctcaaatg ggccgacccc tgaatgggaa aacctccatg cattaaaaga aaagcgttta
      721 aatggaatgc ctaaaacaac ccccaaaagt gaccaggtta ttatgctgca aaatcaatac
      781 gttcgtgaaa tgcgaaaata tggcgtacga ggtttacgtt acgatgcagc aaaacactca
      841 aaacatgaac aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta
      901 cacaatacta atttatttaa cccaaaatat catgaaaaag tcgttatgaa ttacatggaa
      961 tatctggtaa cttgtcagtt ggatgaagaa caaatgtcat cgctgcttta tgaaagagat
     1021 gatttaagcg ccattgattt ttcattgctc atgaagacga taaaagcttt ttcatttggt
     1081 ggcgatctcc aaacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcaaaa
     1141 agacggatat tgattaacat taaacacgat tttcctaaca atggcaatat tttcaatgac
     1201 tttctattta accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg
     1261 ttcagcaggc ctttagttta ctgggatggc caagtattaa aatcaacgac tgaaattaaa
     1321 aattatgatg ggtcgacgcg tgttggcgat gaggcgtggc ttaataaagg ttgctctacc
     1381 tatcagcagc tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc
     1441 gcatttgagg gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg
     1501 aacattaatc actctcctca tgatggacta attataataa ataaaggaaa cgaagaagtt
     1561 gaaggtgcct ggcctaacaa attgcaacct ggaaaatata aaaacatggg gagtaatagc
     1621 gttaacatta ttatta
//
LOCUS       DAZFYC010000324         1587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFYC010000324 DAZFYC010000000
VERSION     DAZFYC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.331"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1587
                     /locus_tag="ACHKW5_005488"
     CDS             <1..>1587
                     /locus_tag="ACHKW5_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO7514620.1"
                     /translation="KTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCPECFKRSDS
                     VHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGSHQQSSQAL
                     QNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHVGIDEWAWH
                     RGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVYATAAREGA
                     PQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSVPAASLRRP
                     ERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGTFPEMSTRP
                     PKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKWRKGWNPPV
                     TTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLALEFYRILK
                     TQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVEGHVNRLKM
                     LKRQMYGRAGFELLRQRVMS"
BASE COUNT          412 a          389 c          426 g          360 t
ORIGIN      
        1 ttaagacact tctacagtta ccttgcggat ggcgatgcag tcgacaaatt attagctctg
       61 acggtataac cctccatctc cacggaaaac gcaaaaaagc acaatgtcct gaatgcttta
      121 agcgtagcga ctctgttcat agttgtcgtc ggcgccggat acagcatcta ccctgctcag
      181 gacatacgct atggcttgta ttttccgtcc gccactggta ctgccgtaac ccggcttgtt
      241 cacgtaaaat ttttgccggg tcgcttgctc ccttcgccgg ttcacaccaa cagtcttcac
      301 aggcgttaca aaatttacaa cgtcaactgg gattaatcgc cggaggtgag gccgggaaac
      361 gggcagcaac ggcagtgggg ctccgttgca gtgctgatac tctccttcgc agggttatca
      421 ataccccgga gacgaaacag tcaggcgcgc ctcatgtcgg tattgatgag tgggcgtggc
      481 atcggggcca ccgttacggt acgttaatcg tcaatcttga tactcaccgt cccctcgtcc
      541 tgcttcccgg ccgtgatcag cgtacgctgg cgacctggtt cagaaaatat ccggaaatac
      601 aaattgtctc gcgtgatcgc agtggagtct atgcgacagc agcacgtgaa ggtgcacctc
      661 aggccagaca ggtggccgat cgatggcatc tgctaaaaaa tattggcgat gcgcttgaac
      721 gaatgatgta cagacatata cctctgatac gtcttgttgc cagtgagttg tcaccaaaga
      781 aatcacctga tccagaacca tctgtgcctg cagcatcgct ccgtcgtccg gaacgcctaa
      841 aacaacaaac ccgcaaaaaa cggcatcagc gttggacaga ggttatggcc ctgcataaca
      901 aggaatgtag tttcagggaa atatcccgta ttacaggcct gtcgcgtgtg acagtcagtc
      961 gctgggtgcg ttcaggaaca ttccctgaaa tgtcaacccg acctccaaag cgagggcttc
     1021 tggacccatg gagggagtgg ttaaaagagc aacgagaaag tagtaattat aacgccagcc
     1081 ggatatggcg ggaaatggtg gcccggggat ttacaggcag tgaaaccatc gtcagggatg
     1141 cggttgccaa atggcgtaaa ggctggaacc caccggttac tactgccgtc agacttcctt
     1201 cagtgtcccg ggtaagccgc tggttgatgc cctggagaat aaccagggat gaagaaaatt
     1261 atgcttcccg atttattagt ctgatgtgtg aaaaagaacc tgagctgaaa atagcgcagc
     1321 aactggcact cgagttctac cgtattctga aaacccaaaa taaatcacag ctaagcagtt
     1381 ggttcaatcg agtccacgaa agcggttcag cagaatttcg gcgcgtggcg gcggggatgg
     1441 aagctgatgc tgcagctata tgtgaggcaa tcagtagtcg ctggagtaat ggtgttgtcg
     1501 aaggtcatgt aaatcgcctg aagatgctga aacgccagat gtatggtcga gcaggatttg
     1561 aactactcag gcagagggta atgagtc
//
LOCUS       DAZFYC010000325         1555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFYC010000325 DAZFYC010000000
VERSION     DAZFYC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.332"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..363)
                     /locus_tag="ACHKW5_005489"
     CDS             complement(<1..363)
                     /locus_tag="ACHKW5_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7514621.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRG"
     gene            complement(353..>1555)
                     /locus_tag="ACHKW5_005490"
     CDS             complement(353..>1555)
                     /locus_tag="ACHKW5_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7514622.1"
                     /translation="FTEFIREGVGVHAFNGEIFVQPVWDTETTQLFRTRFKAVSPKRV
                     DTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPAMLH
                     IFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESDLDTEKAFE
                     YIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGDDLKLQTAQDSDNGF
                     SALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIASRLAT
                     QMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRI
                     EAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAESRRPE
                     EETTPRET"
BASE COUNT          353 a          498 c          376 g          328 t
ORIGIN      
        1 gccccgcacc tggctatctg ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc
       61 agtcattccg gaaaatggcc gcatcccccc cagccggtgc accagcgtgc cggtcaccgg
      121 cagtaccgca ataccgttca ccacccggta aacacgggcc ggtcgtttac ctccggccat
      181 gtactcgtcc gtttcagcca gcattccggg agcatcaagc tgtacctgct gttgtggtac
      241 cgaaagactt gctgccccca tctcgcgccc gagcgcgcaa aagaaaaccc gcgcataggc
      301 gggctccaga agcagcggtt cattgaatgc tgcggcaata atgtgtgaaa gattacgtct
      361 cacgtggtgt tgtctcctct tccggcctgc gactctccgc tatctgctgc tgatacgcct
      421 gcgctatcca caccggacgt gagagtccgg ctttttcccg ctctgcagat tccctgacct
      481 gctggcggaa aatgtcctga taatcctcgc ccatcagcgc cagctctttc tcatacgtgc
      541 tcagtccggc ctcaatgcgc atcactgatt cctgaacctc cttgagcccg tcaatggcca
      601 ttcttccggc tccaatccac tcagcccgtg accaggctga tcgcgcctga taaaaatcaa
      661 aacgtgcccg tggcggacga ataatccccc gaagaagtgc ctcttccagc cagcaggaaa
      721 acatctgcgt ggccagccgg gacgcaataa attttcgccg ccccataaaa tagcgccacg
      781 actcattggc ggatgcgcgg gcacttgaat aactgacctt cgagtaatca cgggacaact
      841 gttcgtagga aacgccaaga ccggcggcga tataccgcag cagcgcctgt tcaagcgccg
      901 aaaatccatt gtctgaatcc tgcgcggtct gaagtttcag atcatcaccg gggaaaaggt
      961 gcggaatttt gacaccgccc agcgtcacgc tattcgtgtc ataccaggtg gagaacttat
     1021 ccagaatatt aataagcgga ttatccttct gcccctgcgg cgcaccggcg atatattcaa
     1081 aggccttttc ggtatcaagg tcactttcaa tcgtcgctgc atacatcgcc ttcactatgg
     1141 ccgactgaag ctgtgttgcc tgcagggaat cgagcatctt cagccgttcc atgacgctgt
     1201 aaaactgatt ggccccacgg gtctgcccgt cctccaccgg ctcgaaaata tgcagcatgg
     1261 ccggacgccc ggtgggaagt tcacgcggga tccgttccca tcgtccactc ccagagaacg
     1321 gaaaatcatc ctcacagata tggtacgcaa cggcacgacc atatcgatcg acctccacac
     1381 cggcccgcag aaaacggttc cccataccgt gtcctggcgt gtccacccgt ttcggactca
     1441 cggctttaaa acgcgtacgg aataactgcg tggtttccgt atcccagacc ggctgcacaa
     1501 agatttcgcc gttaaacgca tgaacgccaa caccttcacg gataaactcc gtaaa
//
LOCUS       DAZFYC010000326         1553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFYC010000326 DAZFYC010000000
VERSION     DAZFYC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.333"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..499
                     /locus_tag="ACHKW5_005491"
     CDS             <1..499
                     /locus_tag="ACHKW5_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7514623.1"
                     /translation="RLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTKEA
                     NVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEGTA
                     ENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALINV
                     IYTWQ"
     gene            558..1472
                     /locus_tag="ACHKW5_005492"
     CDS             558..1472
                     /locus_tag="ACHKW5_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7514624.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
BASE COUNT          445 a          328 c          353 g          427 t
ORIGIN      
        1 aagacttcac aagaggttcc tgttagctac gttttgcgcg ttattcacag caactctcca
       61 ggccgccgat gtcactatca ctgttaatgg tcgggtagtc gctaaaccct gcactattca
      121 aaccaaagaa gctaacgtta atctcgggga tctttatacg cgcaatctgc aacaacctgg
      181 ttctgcatct ggctggcaca atattacttt gtcattaacc gattgtccgg ttgaaacaag
      241 tgcagtgacg gcaatcgtga caggttcaac tgacaatacg ggttattaca aaaatgaagg
      301 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
      361 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      421 aagagctatc acggtgaatg gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt
      481 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      541 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      601 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      661 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      721 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      781 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      841 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      901 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      961 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgctcgt attcatatgt
     1021 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
     1081 ataacaatgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
     1141 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
     1201 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
     1261 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt ctctctgatg cgtaatggaa
     1321 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1381 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt tgcagccgga accgtccagt
     1441 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgatcttag tctatgcgct
     1501 ccattcacga aaattgcgcg gataattatt ttgtgaaggc tattatccta cac
//
LOCUS       DAZFYC010000327         1551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFYC010000327 DAZFYC010000000
VERSION     DAZFYC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.334"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1476
                     /locus_tag="ACHKW5_005493"
     CDS             <1..1476
                     /locus_tag="ACHKW5_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7514625.1"
                     /translation="DITDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYF
                     QPVMDNMTGYRNNPDFVAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAAD
                     GSITGTPRNSRIASFWMEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDI
                     GRPYLPRSATEQRKSELLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGY
                     GEQGERWIVDRYNIRHSLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDV
                     RMRLMAMAVDTGGEAGVTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDN
                     TGRSARRARASGDVALWLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTY
                     EERGSDGKWRKPGRGANEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQE
                     RSGETVSPELTAGAEKRRRRKKKLWTERAEDNPWITSGGWL"
     gene            1473..>1551
                     /locus_tag="ACHKW5_005494"
     CDS             1473..>1551
                     /locus_tag="ACHKW5_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514626.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSV"
BASE COUNT          350 a          378 c          514 g          309 t
ORIGIN      
        1 gatatcaccg ataccaaatg gcgttgtggt ggcgcacatg aggcaccgcc aacaacgggg
       61 atcctgtcac tgtataaccg gggagaccgc cgccggtggt actggccgtg tccgcactgc
      121 ggggaatatt ttcagccggt gatggataac atgaccggat accggaataa cccggatttt
      181 gtggctgccg gtcaggctgc ccgtctgatg tgtccgcatt gtcgcgggct gatcgcccct
      241 gagcagaaac gcgaactgaa taaccagggg atctggcttc gtgaaggtga acgggcggcg
      301 gcggacggca gtatcaccgg aacgccacga aactcccgga ttgcgtcatt ttggatggag
      361 gggccagctg cggcgtttca gacctgggaa caactgattt ttaaactgct ggcggcagaa
      421 gaagagtatg agcgaaccgg cagtgaagag accctgaaag cggtggtgaa caccgatatc
      481 ggacgaccct atctgccccg ttcagccacg gaacagcgta aaagtgaact gcttgaacag
      541 cgtgccgagc cgtttccccg gcgatctgtg ccggatggtg tgcgttttat tgaggcaacg
      601 gttgacgtac agggcggtaa aaatcgccgt tttgttgtgc agatcaccgg atacggagag
      661 cagggggaac gctggattgt tgatcgctac aacatccggc attcactgcg ctgcagtccc
      721 aacggtgaaa gtctgccggt tgatccggcg gcatatccgg aggactggga tttgttgctg
      781 acggatgtgt tccataaaac atggccgctg gcttctgatc cggatgtgcg catgcgtctg
      841 atggccatgg cggtggatac gggaggggaa gccggggtga cagataacgc ctatcgtttc
      901 tggcgtcgtt gccggagtga cggactgggc aacagggtgt ttctgttcaa gggggatgga
      961 cttcgccgtg acaggctgat taaccgaacc ttcccggata ataccggcag aagtgcccgc
     1021 cgtgccagag ccagtggcga tgtcgcgctg tggctggttc agacggatgc gtttaaggat
     1081 cgtgtaaata atgccctgtg gcgtgacaca ccagggccga actatatcca ctttcccgac
     1141 tggctggggc ggtggtttta cgatgagctg acctatgaag agcgcggcag tgacggaaaa
     1201 tggcgaaaac cgggcagggg cgctaacgaa gcgtttgacc tgctggttta tgcggatgcg
     1261 cttgccgttc tgcatggtta cgaaaagatc cgctggccct ccgcaccgga ctgggcacag
     1321 cgggaaacgt ggctcgtctt cccgcaggag cgttctggtg aaacggtatc cccggaactg
     1381 acggccgggg cagaaaaacg ccgtcgccgg aagaaaaaac tgtggacgga gcgtgcggaa
     1441 gataatccat ggataacatc aggaggctgg ttgtgagcac agaagaagcc agagaaatga
     1501 tacagcggta ccgtgaagcg gaaatggccg tactggaggg aaagtctgtc a
//
LOCUS       DAZFYC010000328         1527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFYC010000328 DAZFYC010000000
VERSION     DAZFYC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.335"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..67)
                     /locus_tag="ACHKW5_005495"
     CDS             complement(<1..67)
                     /locus_tag="ACHKW5_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514627.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPI"
     gene            complement(120..524)
                     /gene="ycgX"
                     /locus_tag="ACHKW5_005496"
     CDS             complement(120..524)
                     /gene="ycgX"
                     /locus_tag="ACHKW5_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO7514628.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(745..1476)
                     /gene="bluR"
                     /locus_tag="ACHKW5_005497"
     CDS             complement(745..1476)
                     /gene="bluR"
                     /locus_tag="ACHKW5_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO7514629.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          401 a          341 c          330 g          455 t
ORIGIN      
        1 ttatgggtag caataaaagg acagtattta tcatccatag ggatagtctc tgtactttta
       61 ttcccattat gctaatgcct tactgaatta tgaagcattt cttaagcatc caactttagc
      121 tagattaatg gtttattatt ttctacatct tcaatatata aaagcgtatt atcaatggcg
      181 tagtaactgc gtttgttatg attaacatca gtaacccacc ggaaaacgcc cgcgcctgct
      241 agtgttgaac agtattcccg aaatgtagat tttccgcaaa tatgaagcaa tgcggcctct
      301 tttattttag cagggttctt ggtcgtacta acttttaaca ggttcctggt tcctcttaat
      361 aacaaaaccg tgtcatcgtg agtaataatt ctgatgttat ccgtagccag ataataaatg
      421 taatgtgcaa tacggtgatg ttttaattct gaataaaacc aggagaagtt ttgctctttt
      481 ctcacttgct caaacatctt ttgaaaaaca acgacctgat ccatcaggat aataacctct
      541 tgttagttgt gagactgcgt agtgtgcacg atcggtttta ccacttcaat ctggtctgtc
      601 ctttggctgt gatatgtaca gagtgtgata gagggaatat ctgaattctc ccggtgagca
      661 ttttgcaacg gaccagctcc ggtacaaacg ctgttgtggg ttcagattat aacattctgt
      721 ctaaggggcg ggataaaggt gaaattaggg ggcatgaaag atgactttat aaccttgctc
      781 accccagtgt tgtaaaagtt cgttttgcct tctcgttggt gccatgcctg tccagacaat
      841 caatgtttgc gtcgggaaca gttccgggcg cggcgattca atgggatcag caagaacaga
      901 aatgtgccat cctgacaatg ataaacgcca tgcttccagc cacaatcgtg ctctctcttc
      961 aacattccac gccatcagaa tggcttcttt accgggctta cggcgcattt cgaaaagcga
     1021 ggttgctgcg tattcaatta atgcgccgtc aaacatactg ctcataatgc gggaggtgtt
     1081 gtgatcaagc acgagacgct ggcgaacagg aaggtaaaca tgattaatca attgatctac
     1141 tgggtactct cgacccagtg aaataattct cgcgcgtagt ttggcaggat tagccatgcg
     1201 aagaattgac atcatctctt cttgcaggcg gctccagtca tcttccgtat cctggctggt
     1261 ggtttccagt aatgctttaa ctttgcctac agggacgcca ttacttatcc aacgcttgat
     1321 ctcttcgatg cgttgtatgt cttcttcatc aaagagtcgg tgtccgcctt cactgcgctg
     1381 tggttttaac aaaccgtagc ggcgttgcca ggcccggaga gtgacaggat taatcccgca
     1441 acgttcagca acatcaccaa tgctgtaata agccacaatt cctccttgcg gtcacaaatc
     1501 tccgtcgcct gtacacgacc caataat
//
LOCUS       DAZFYC010000329         1522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFYC010000329 DAZFYC010000000
VERSION     DAZFYC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.336"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..363)
                     /locus_tag="ACHKW5_005498"
     CDS             complement(<1..363)
                     /locus_tag="ACHKW5_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7514630.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRG"
     gene            complement(353..>1522)
                     /locus_tag="ACHKW5_005499"
     CDS             complement(353..>1522)
                     /locus_tag="ACHKW5_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7514631.1"
                     /translation="HAFNGEIFVQPVWDTETTQLFRTRFKAVSPKRVDTPGHGMGNRF
                     LRAGVEVDRYGRAVAYHICEDDFPRSGSGRWERIPRELPTGRPAMLHIFEPVEDGQTR
                     GANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAGAPQEQKD
                     NPLINILEKFSSWYDTNNVTLGGVKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYI
                     AAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIAARLATQMFSCWLEEAL
                     LRGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKEL
                     ALMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAYQQQIAESRRPEEETTPRET"
BASE COUNT          338 a          487 c          372 g          325 t
ORIGIN      
        1 gccccgcacc tggctatccg ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc
       61 tgtcattccg gaaaatggcc gcataccccc cagccggtgc accagcgtgc cggtcaccgg
      121 cagtacagca ataccgttca ccacccggta aacacgggcc ggtcgtttac ctccggccat
      181 gtactcgtcc gtttcagcca gcattccggg agcatcaagc tgtacctgct gctgtggtac
      241 cgaaagactt gctgccccca tctcgcgccc gagcgcgcaa aagaaaaccc gcgcataggc
      301 gggctccaga agcagcggtt cattgaatgc tgcggcaata atgtgtgaaa gattacgtct
      361 cacggggtgt tgtctcctct tccggcctgc gactctccgc tatctgctgc tgatacgcct
      421 gcgctatcca caccggacgt gagagtccgg ctttttgccg ctctgcagat tccctgacct
      481 gctggcggaa aatgtcctga taatcctcgc ccatcagcgc cagctctttc tcatacgtgc
      541 tcagtccggc ctcaatgcgc atcaccgatt cctgaacctc cttgagcccg tcaatggcca
      601 ttcttccggc accaatccac tctgcccgtg accaggctga tcgcgcctga taaaaatcaa
      661 aacgcgcccg tggcggacgg ataatccccc gaagaagtgc ctcttccagc cagcaggaaa
      721 acatctgcgt ggccagccgg gccgcaataa attttcgccg ccccataaaa tagcgccacg
      781 actcattggc agaggccctg gcacttgaat aactgacctt cgagtaatca cgggacaact
      841 gttcgtagga aacgccaaga ccggcggcga tataccgcag cagcgcctgt tcaagcgcag
      901 aaaatccatt gtctgaatcc tgcgcagtct gtagtttcag atcatcacca gggaaaaggt
      961 gcggaatttt gacaccgccc agtgtcacgt tattcgtgtc ataccagctg gagaacttct
     1021 ccagaatatt aataagcgga ttatccttct gctcctgtgg cgcgccggcg atatattcaa
     1081 aggccttttc ggtatcaagt tcactttcaa tcgtcgctgc atacatggct ttcacaatgg
     1141 ccgactgaag ctgtgttgcc tgcagggaat ccagcatctt cagccgttcc atgacgctgt
     1201 aaaactgatt agccccacgg gtctgcccgt cctccaccgg ctcgaaaata tgcagcatgg
     1261 ccggacgccc ggtgggaagt tcacgcggga tccgttccca tcgtccactc ccggagcgag
     1321 gaaaatcatc ctcacagata tggtacgcaa cggcacggcc atatcgatcg acctccaccc
     1381 cggcccgcag aaaacggttc cccataccgt gtcctggcgt gtccacccgt ttcggactca
     1441 cggctttaaa acgcgtacgg aataactgcg tggtttccgt atcccagacc ggctgcacaa
     1501 agatttcgcc gttaaacgca tg
//
LOCUS       DAZFYC010000330         1514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFYC010000330 DAZFYC010000000
VERSION     DAZFYC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.337"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..222
                     /locus_tag="ACHKW5_005500"
     CDS             7..222
                     /locus_tag="ACHKW5_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7514632.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            227..760
                     /locus_tag="ACHKW5_005501"
     CDS             227..760
                     /locus_tag="ACHKW5_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7514633.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            977..1162
                     /locus_tag="ACHKW5_005502"
     CDS             977..1162
                     /locus_tag="ACHKW5_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7514634.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            1248..1472
                     /locus_tag="ACHKW5_005503"
     CDS             1248..1472
                     /locus_tag="ACHKW5_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514635.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          370 a          351 c          438 g          355 t
ORIGIN      
        1 cggggtatga accagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg
       61 ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca gtgggcggca
      121 ataggcgtgc tggggagtct gctgtttggt ctgctgacgt acctgacgaa cctgtatttc
      181 aaaatcagag aggacaggcg taaggcggca cggggagagt aagctgatga gcaggaaact
      241 ccgctatggt ttatcggctg ccgttctggc gctgattgcc gcaggtgctt ctgcgcctga
      301 aatcctcgac cagtttctgg atgaaaagga aggcaaccac accacggcat accgtgatgg
      361 tgcgggtatc tggaccatct gccgaggcgc catcatggtg gatggtaagc ctgtgattcc
      421 tggcatgaag ctgtcgaagg aaaaatgcga ccgggttaac gctatcgaac gggataaggc
      481 gctggcatgg gtggagcgta atataaaagt tccactgacc gagccgcaaa aagcaggtat
      541 cgcgtcattc tgtccgtaca acattggtcc cggtaagtgt ttcccgtcta cgttttacag
      601 acgaattaat gcaggtgatc gaaaaggtgc ctgcgaggcg attcgctggt ggattaagga
      661 cggtggcaga gactgccgta ttcgttcaaa taactgctac ggacaggtta ttcgtcgtga
      721 ccaggaaagc gcattagctt gttgggggat agatcagtga gaagagtaac cgcgattatt
      781 tacgctctgg ttatctgcat catcgtctgt ctgttatggg ctgttaatca ttaccgcgat
      841 aacgccatca cctacaaagc gcagcgcgat aaaaaagcca gagagctgga acaggcgaat
      901 gccaccatta ctgacatgca ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac
      961 tcgaaggaat tagccgatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgct
     1021 ggtcgtaagc gcctgcggat caacgccacc tgctccggta ccgtgcgtga agccaccggc
     1081 acctccggcg tggataatgc aaccggcccc cgactggcag acaccgctga acgggattat
     1141 ttcatcctca gagaacggtt gatgatgatg cagaagcagc tggaaggggc gcaggaatat
     1201 atccgcactc agtgtattaa ctagtatttt tgttatccgg agaatgcatg aagaaattac
     1261 tggtaaccgt aaagcctttt cagggaacaa ttccgttccg tgttttgcag cgtggtcgtg
     1321 ttcttgttga aggttcgttc agtggtaaat gtacgcaatt acactcccgg acctttcagg
     1381 tgaatgccac gaatgaagag ctaaccgttg agtgtacgat gaatgccgct aaatgccgca
     1441 tggtatccgc tgcattacag ccagtgtgtt gagcgacctt attatccatg cgcggtattg
     1501 tcgccgtatt cccg
//
LOCUS       DAZFYC010000331         1492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFYC010000331 DAZFYC010000000
VERSION     DAZFYC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.338"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..203)
                     /locus_tag="ACHKW5_005504"
     CDS             complement(<1..203)
                     /locus_tag="ACHKW5_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscE/YscE/SsaE family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO7514636.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRIIEIMLI"
     gene            complement(218..589)
                     /gene="ler"
                     /locus_tag="ACHKW5_005505"
     CDS             complement(218..589)
                     /gene="ler"
                     /locus_tag="ACHKW5_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO7514637.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
BASE COUNT          441 a          319 c          250 g          482 t
ORIGIN      
        1 ctgattagca ttatctctat gatcctgatt gccgcatcgc aggcaatgag cagttccttt
       61 gcttcattgt aggtaacttt atccaagggg tgtttcattt tttcatggat ttcgttcttt
      121 ttgtcgttta ttttttcaat gaaaatattt gcggcttctt tattttttat tatttcgtct
      181 tccagttcag ttatcgttat catttaatga tttcatgtta aatatttttc agcggtatta
      241 tttcgtcttc ggtgtccttc acaagaaaat cttctttctt cattccattc aacagtgctt
      301 ctttaagcca gcgtggctgt cggccaactc ccgaccaggt ctgcccttct tcattgcgat
      361 aataaactcc tttcgatgag ttccggcgag caaggccatc atcaggcaca ttagtatatc
      421 ccagctcttg tagggttata ttgtaataag taatctgctt ttgaatcgac tcaattacac
      481 tttgaatttc ctgctctcgc agtcgctttg cttcctgttg taacacttca atttgctcaa
      541 taagctgaat gtatgggctt gttgtgtgcg aattagtttc catattcata ataaataatc
      601 tccgcatgct ttaatatatt aagctattag caatcttatc cttatcagga aggatcaaca
      661 attaatcgaa tgatataaat tatgtgagat aacgttttat ctatcaaatt agaacacatc
      721 tacttcatca aacaaaatcc ttataaaagt tattaatcac cgatgtataa tgtgtaaaac
      781 acattatcat taaatgtcaa caaaaaaatc atatttctta aaaatcattc aaaatcaata
      841 aagtagaaga acttcatctc tttagcaaac gccattaaca attgcctttc catttagtta
      901 atcgttttgt tttctttaag attagtccta ttagacaaat agcaaaatgg aataactaca
      961 gcactaagac gttccgctat atagctatta cctgttcccc tgacataagg agcagatata
     1021 cctcaacccc ctattcccca gtgctgaccg ggagcggcgg cggtataaaa aggaacaagg
     1081 aacaaatggc agaacaagca accatgtttg tcccccccgc ataaccgtac cattcgcatt
     1141 aagaaatatt cccatatcta tcccgcaaaa gaggacccgt catacatcaa gtccgtttta
     1201 ctgggcatca gattatcgcc gtaattagct cagtgaaagc cgggtaaacg gttagcaagc
     1261 tcagtggctc aaaagtgcat agcgctaaga tgggcctggt tacacctgcc ctttgctgca
     1321 gatcgtgttg atcggtgtga tgccgccaca cgtttaagtc aatctggaaa actcacaatg
     1381 cgctcttcta taaagaggac tctttgataa agtaacccga aaagggaaag ctaactgata
     1441 tcaatgtacc accttagtaa tcatccctta tatacagaca aaaagaagtt gc
//
LOCUS       DAZFYC010000332         1492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFYC010000332 DAZFYC010000000
VERSION     DAZFYC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.339"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..210
                     /gene="ydcD"
                     /locus_tag="ACHKW5_005506"
     CDS             <1..210
                     /gene="ydcD"
                     /locus_tag="ACHKW5_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFO7514638.1"
                     /translation="FNDILYSVRLKNISKLENMEFCAKKQRVYFSDKNKKASYKIINY
                     GDYYDVDYYDNNLKNEVFDWIGKWS"
     gene            complement(125..310)
                     /locus_tag="ACHKW5_005507"
     CDS             complement(125..310)
                     /locus_tag="ACHKW5_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514639.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            391..1492
                     /locus_tag="ACHKW5_005508"
                     /pseudo
     CDS             391..1492
                     /locus_tag="ACHKW5_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          554 a          198 c          344 g          396 t
ORIGIN      
        1 ttcaatgata ttctttatag cgtgcgatta aaaaacatta gtaaactgga gaatatggag
       61 ttctgcgcta aaaaacaacg ggtgtatttt agtgataaaa acaagaaggc tagttataaa
      121 ataattaatt atggagacta ttacgatgtt gattattatg ataacaattt gaaaaatgaa
      181 gtttttgact ggattggtaa atggtcataa tgaacaacac cagaaagaga aaaatataaa
      241 atacatacag aaagacatga gagcaaggga cggggcaggg caagatcatg aaaattgtga
      301 tgtaaatcac gattttcatc ttggctttaa cgcctacagg tgatcaaaat ttaacctttg
      361 gaataactaa aaagataaaa aaggacccag gtgagtattc aaagtttgct tgattatatt
      421 tcagtgatcc ctgatatacg acaacaagga aaggttaaac ataaattatc tgctattttg
      481 tttctcaccg tatgtgcagt aattgcaggt gccgatgagt ggcaggaaat tgaagatttt
      541 ggacatgaaa gacttgaatg gctaaagaaa tatggtgatt ttgataatgg cattccggtc
      601 gatgacacca ttgcacgcgt tgtgagtaac attgacagtt tggcctttga aaagatgttt
      661 attgaatgga tgcaggagtg ccatgaaatc actgatggtg aaattatagc aatagatgga
      721 aagaccataa gaggctcctt tgataaggga aaaagaaaag gagcaatcca tatggtgagt
      781 gcattctcga acgaaaatgg tgttgtactg gggcaggtga aaacggaagc caaaagtaat
      841 gagattacag ccattccaga gttgcttaac ctactgtatt taaagaaaaa tttgataacc
      901 attgatgcta tgggctgtca gaaagatatc gcttcgaaga tcaaagataa aaaagcagat
      961 tatcttctgg cagtaaaagg caatcagggg aaattacatc atgcattcga ggaaaaattt
     1021 cctgtaaatg tgttttctaa ttataaaggc gattcgttta gtacgcagga gataagtcat
     1081 ggaagaaaag aaacacgttt gcatattgtc agtaacgtaa cgcctgaact tttgtgattt
     1141 tgaattcgaa tggaagggat taaaaaagct ttgtgtagca ttgtcattca ggcagaagaa
     1201 agaagataaa tcagcagaag gtgtaagcat ccgatattat atttcatcaa aggatatgga
     1261 tgctaaagaa tttgcacatg ctatcagagc gcactggctg atcgagcaca gtcttcattg
     1321 ggtgttagat gtaaaaatga atgaagatgc cagccggata agaagaggaa acgcagccta
     1381 aataatatct ggaataaaga agatggcact gaatttatta agagattgca aagacattaa
     1441 gggtgaagag gaaaagaaag aaggctgcgt taaacacagg gaaagatcat ca
//
LOCUS       DAZFYC010000333         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFYC010000333 DAZFYC010000000
VERSION     DAZFYC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.340"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(430..603)
                     /locus_tag="ACHKW5_005509"
     CDS             complement(430..603)
                     /locus_tag="ACHKW5_005509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514640.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            781..1491
                     /locus_tag="ACHKW5_005510"
                     /pseudo
     CDS             781..1491
                     /locus_tag="ACHKW5_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          357 a          418 c          321 g          395 t
ORIGIN      
        1 acacactgac aaaaaaccaa gcatggtaat aaaccgaact ccccacaaac ttctgttgcc
       61 tcagatactc cccccctttc ctcaggcaca aatgttgttc cataacgaac cagccctgtt
      121 cctttaaact ctgcatcaat ggcataatgt atactcacca gactgagttt gctcctgcat
      181 cattatttcg atacgttaat gcattgttca ttgcatccgg cattccccat cgaatattta
      241 ctttgttata tcagtcagcc cataaccagg taaattttta aggcttactg actgaatatt
      301 caaaatgtgt tacatgttat caaaccttta aagaataacc tgctcttctt cattggccgg
      361 atttagctca atcttgtcag caaaactaat ataaagccgt tcataaagag aaggatgtag
      421 cttcgttatt tatcttttac atgtaacagt gccaggggat attccgcaca tctctgtggc
      481 tatcatcccc accacgtctc cagtggcagc acctgcggca ctggcacggg cgttatttcc
      541 ctgtaccacc gccagtgcag cattcaccac ggcatgggct gccacatttg cagtcttatt
      601 caccacacct gtaacctgtc tcttatacac aaatccccac ccggggattt gtgatgtctg
      661 tcagtctgct ttagggggac tctttccatc cggtagtttc cttcctcccc ggcagcgatt
      721 ttttgaactt tttcttcacc cgttgatctt ctcttatatt tcattttctt cgggtctgtt
      781 atggtacttt ctgattgtta ttcactggct aacgatcagt ctggacatgc ccgtctcggt
      841 gatccgcgtc gtacacgacg ccttgtgtca ctgacctctt ctctcgctca gcatgcaggt
      901 ctttctattg tgaaatcatc ccacttcact gctcaggttg aaggtgcgta ccgcctgata
      961 cgtaacccgt cggtgtcccc gtaagccatt gccgaagccg gatttactgc cactgcaagg
     1021 gcatgtgagg cacatccgct gctcctcgcg ctggaagata ccaccaccat taacttcggt
     1081 cacagtacgg catttgatga cctggggaat accaccacta acccgaagac gcacgggctg
     1141 ctggctcact ctgtcctgat gtacgccccc gacagcgccc tgccagtcga tcttatcgaa
     1201 cagcagtgct ggagtcgtgc cacggacacc tacggtgtta agcaccagcg aaaagaacgt
     1261 ccctgtgaag aaaaagaaag ttaccgctgg cagcaggctt cggagcgcat ggccgaacgg
     1321 ctgggggaga tacagaaaca ggtaatcact gtctgtgacc gggaagcgga tatctggcat
     1381 tacctgcatt ataaagtcag ccgcgggaag ccacacgctg aatgtgatgc aaaaaggcgg
     1441 gcgggcagcc cgccaggccc ggatgctcat cagttacagc gaagtcagca t
//
LOCUS       DAZFYC010000334         1479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFYC010000334 DAZFYC010000000
VERSION     DAZFYC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.341"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..528)
                     /gene="wzy"
                     /locus_tag="ACHKW5_005511"
     CDS             complement(<1..528)
                     /gene="wzy"
                     /locus_tag="ACHKW5_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158642092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O123/O186 family O-antigen polymerase"
                     /protein_id="HFO7514641.1"
                     /translation="MYLTTNYCDFLSRTRIVRRIFLLVYLISNVYAYIVFTETGVLIG
                     DYQGVLIIYKDQVVYLLMLTIMSYYIVCGPVFNCLSKVKIRLRTFKGLNSFAYILFFF
                     QILFAVFNISTGSNAAGTDFQTGGVIRLLWLFLPVDYLFYIYYFVGREKKVSKIYLAN
                     VVIFILSMLSRGWLGW"
     gene            complement(512..>1479)
                     /locus_tag="ACHKW5_005512"
     CDS             complement(512..>1479)
                     /locus_tag="ACHKW5_005512"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7514642.1"
                     /translation="NYIRQCLDGVFSQKTNFEYEVIIRDDCSTDNTYLTIMEYIDTLD
                     EEKKKNIKITVLDGTKNIGANNNFIETFKTSVGQWLAICEGDDYWCDQGKLQKQYDYA
                     ISHSDCSLVVHPALVSENNVIRKTSWACMNKTINQLSDVIRAKGQFSPTGSYFFKREI
                     LNVLPLWFSTAPVGDYYMEIFATSLGSCHTIPDAMSVYRINSTGSWSDLLKKDRNGQR
                     IINTYLSQLEYLDKLAEIFPSCVDDITIKRSHTQYAAAMGYLANGDYTNFRILMDKSD
                     TNGWYDNMHSIFYYLRNSRALSMLMFRFKPAIKSAVMWGRRVFNN"
BASE COUNT          509 a          281 c          217 g          472 t
ORIGIN      
        1 ccacccaagc catcctctac ttaacattga tagaataaaa ataaccacat tggccagata
       61 tattttactt acttttttct ctcggcctac gaaataatat atataaaata gatagtcaac
      121 tggtaaaaaa agccataata gtcttatgac tccaccagtt tgaaaatcag tgcccgcggc
      181 attcgagcct gttgaaatat taaaaacagc aaataggatt tggaaaaaaa acaggatata
      241 agcaaaagag tttaagcctt taaatgtcct tagtctaatt tttacttttg atagacagtt
      301 aaaaacagga ccacaaacaa tataatatga cattattgtt aacatcaata aatacacaac
      361 ctgatcttta tatattatga gtactccttg ataatcccct attaatactc ctgtttcagt
      421 aaacactata taggcgtaaa catttgaaat caaataaact aacaaaaata tgcgcctaac
      481 gattcgagtt cgggacaaaa agtcgcaata attagttgtt aaatacacga cgcccccaca
      541 tgacagcaga tttaattgca ggcttgaatc tgaacattaa catggaaagc gccctgctat
      601 ttcttaaata ataaaaaata gaatgcatat tatcatacca gccgtttgta tctgacttat
      661 ccattaatat acgaaaatta gtataatcac catttgcaag ataacccatt gctgctgcat
      721 attgtgtatg cgatcttttt attgtgatat catcgacaca agaaggaaag atttcggcaa
      781 gtttatccaa atactccaat tgagaaagat aagtgttaat aatccgttgg ccatttctat
      841 ctttttttaa caggtcagac caagaccccg ttgaattgat tctataaact gacatagcat
      901 caggaattgt atgacaagat ccaagcgatg tagcaaagat ttccatgtaa tagtcaccaa
      961 caggagctgt tgaaaaccac aatggtagaa catttaagat ttctcgtttg aaaaaatagg
     1021 agccagttgg tgagaactgt ccttttgcac gtattacatc gctaagctga tttattgttt
     1081 tattcataca cgcccaagat gttttacgta ttacattatt ttcacttacc agagcgggat
     1141 gcacgactaa tgagcaatca ctatgtgaaa tagcgtagtc gtactgtttt tgtaatttcc
     1201 cctgatcaca ccaataatca tctccctcac atatagctag ccattgacct actgaagtct
     1261 taaatgtttc gataaaatta ttatttgctc caatattttt cgtgccatca agtaccgtta
     1321 tttttatgtt cttttttttc tcttcatcta aagtgtcaat atattccatt attgttaaat
     1381 atgtattatc ggtactgcaa tcatcacgta taataacctc atactcaaaa tttgttttct
     1441 gagaaaagac accatccaag cactgtcgta tatagtttt
//
LOCUS       DAZFYC010000335         1473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFYC010000335 DAZFYC010000000
VERSION     DAZFYC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.342"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..327
                     /locus_tag="ACHKW5_005513"
     CDS             <1..327
                     /locus_tag="ACHKW5_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7514643.1"
                     /translation="TVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQRLKLSLSGRLA
                     IKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVRGQPVSGGRLGVKI
                     YKIESE"
     gene            336..866
                     /locus_tag="ACHKW5_005514"
     CDS             336..866
                     /locus_tag="ACHKW5_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO7514644.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKRLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            1120..1326
                     /locus_tag="ACHKW5_005515"
                     /pseudo
     CDS             1120..1326
                     /locus_tag="ACHKW5_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1323..1454
                     /locus_tag="ACHKW5_005516"
     CDS             1323..1454
                     /locus_tag="ACHKW5_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7514645.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLL"
BASE COUNT          389 a          280 c          433 g          371 t
ORIGIN      
        1 acggtgaaca cttactggcg acgccgtggc agcacatatt ttgtatcaaa agccggggag
       61 cgttatcgcc gggcagtggc gcttattgtt cgccagcagc gactgaaatt aagcctgtcc
      121 ggacggctgg caataaaaat tattgcagag ccaccggata agcgtcgtcg tgacctggac
      181 aatattctga aagcaccgct ggatgcgctg acgcatgcgg gagtgctcat tgatgacgag
      241 cagtttgatg aaatcaatat tgtacgtggt cagccagtat ctggtggacg gctgggtgtg
      301 aagatttaca aaattgagag tgagtgagcg taaatatgat atatccggaa attacaggca
      361 aaagcggcga acatttacgc ctgaacacgc tggaagcagt ctggatccag gggaaattac
      421 ggatgtgggg gcggtggtcg tatatcggtg ggggtaaatc cggaaatatg tttaaccggt
      481 tactggtttc gaaaaggctg acgaaaacag cagttaatga ggttttacgc agcatgaaga
      541 aatccgggct ggaaaaaccg gaacttgagg cattttttcg ggatatgacc agagggaagc
      601 agaagagctg gttgtcacat tgtacagaca cagaggcgtt gattattgat cgcgttatca
      661 gtgaggtgct tggggaatat cccgggctaa tcaatattct ccggcaaagg tacgaaggac
      721 ggggaatgag taagagaaaa atggcagaat gtttaaatcg tactcacccg gaatggtgtt
      781 tcagcacatg tgagaaacgt attgcaggtt ggttagccgt ggctgaacac atgctttatg
      841 tacctatgca cgattcattt cgataaaaaa aagcttgctt ttttacgcag aaacagcttg
      901 aattcctgta agctccgcaa agctgtatcg cgaggcgaaa tgcaagtttt ttcgcacaag
      961 gaagccaccg gaaggtggtt tttttgtgtc cgtaatatac agcagcgcaa taaattcgct
     1021 ggtggttatt aataccgttc tttcagcttg ctggcttttt cgacaagagt tattggtgtg
     1081 tcacgttaac cggaaaaagg aaagtttgag aaacgcgatc tggcacaggc ggttattaat
     1141 gctgcctacc tggtggcctg tgcagatggt gaatgtgagg cttcctagaa atcgaagatc
     1201 gaacaggtac tgcgtaatca gcctgcgctg tccgcgttta cgtcagaaat aattgagccg
     1261 gaagagcgca aggtgctgga agagattgcc ggtgttctgg gtcttcgtct ggagaatcac
     1321 ctgtgacggt aaaactgcgc ctggctgtgg ctgcactcct gctgtttctg gtggtgatgg
     1381 tggatttcac cagcagaatc atgtcggtgc tggcggatgg ggtgctggtc tgcggcattg
     1441 tggtattgct gtagccggtg ataaaaagaa aca
//
LOCUS       DAZFYC010000336         1454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFYC010000336 DAZFYC010000000
VERSION     DAZFYC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.343"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..309
                     /locus_tag="ACHKW5_005517"
     CDS             <1..309
                     /locus_tag="ACHKW5_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514646.1"
                     /translation="IIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHEPV
                     TDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKKSTAGISIS
                     "
     gene            378..1001
                     /locus_tag="ACHKW5_005518"
     CDS             378..1001
                     /locus_tag="ACHKW5_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7514647.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQFSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSASGDWRTDGFIVGIGYKF"
     gene            1066..>1454
                     /locus_tag="ACHKW5_005519"
     CDS             1066..>1454
                     /locus_tag="ACHKW5_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7514648.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARHAEEAKKNAGEAETS"
BASE COUNT          335 a          335 c          446 g          338 t
ORIGIN      
        1 atcattcccc cggtgctgtt tcgcgggacg aaacaccaga atttcaacag cccgaatcag
       61 cagtcgtact ggtattccac ctgtaagctg caggtgctga agaacggggt tgagattttc
      121 catgaaccgg taacggatgt cagccgggtg ttctcatcgg tgatagatat gccggcaggg
      181 cggggtcatg tcaccctgac gtttaatgtg tcgtcggccg gtgcgaacaa ctggacgccg
      241 acaacgtaca tcagtgattt actggttgtg gtcatgaaaa aatccactgc agggatcagt
      301 atcagctgac ggtttattaa cccggacggg cacctcagga ggtgcctttt ttattgactg
      361 aaaacaaaga ggtaatcatg cggcatttat acgcaacgat attattgttt actaccctgc
      421 tggcaggaat tgcctttcct gcacaggctg aaagcggaca cggtgcattt tccgtgggat
      481 atgctcaggt tcacccgggc ggcgtaccgg cattgtccgg taccggtgcg cgtgcaggtg
      541 atttaaaagg gattaacgtg aaataccgtt atgagttcac ggatcacctg ggcggcattg
      601 tcgcgctgag ttatgcatcg gtgaagaaaa gtgacacgat gaagacgggt gaaaatacct
      661 tccattatga aagcctgcgc ggtcgttatg tcagtctgat ggccggccct gtctggcagt
      721 tcagtgagcg ggtcagtctc tatggcatgg ccgggatggc gtacacccgc tggtctgaca
      781 gtgttcagga ttaccggcgt gatgaagtga aaccggggta tgtgaaggag accaccaccg
      841 ccagtgatgg tcatactgcg cgtcatctgt cgccggcctg gaatgccggg attcagttca
      901 gtcccgtaga gacggtggtt attgaccttg cttatgaagg ttccgccagt ggcgactggc
      961 gcactgacgg cttcatcgtg ggtatcggct ataaattctg attagccagg taacacagtg
     1021 ttatgacagc ccgccggttc aggcgggctt ttttgtgggg tgaatatggc agtaaagatt
     1081 tcaggtgtac tgaaagacgg cacaggaaaa ccggtagaga actgtaccat tcaactgaaa
     1141 gccagacgga ccagcagcac ggtggtggtg aacacggtgg cctctgaaaa tccggatgaa
     1201 gccgggcgtt acagcatgga cgttgagtac ggtcagtaca gcgtcattct gttggtggaa
     1261 ggattcccgc cgtcacatgc cgggaccatc accgtgtatg aagattctca acccggtacg
     1321 ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc tctgcgccgt
     1381 tttgaactga tggtggaaga ggtggcgcgt cacgctgagg aggcgaagaa gaatgccgga
     1441 gaggcggaga cgtc
//
LOCUS       DAZFYC010000337         1446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFYC010000337 DAZFYC010000000
VERSION     DAZFYC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.344"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..1438
                     /gene="dgcT"
                     /locus_tag="ACHKW5_005520"
     CDS             80..1438
                     /gene="dgcT"
                     /locus_tag="ACHKW5_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7514649.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          413 a          229 c          323 g          481 t
ORIGIN      
        1 tttcatattg acaacagtac agttcttata tctattaata atagaaaggg atctacaacc
       61 tacagattgg tgtagcttta tggaaaaaga ctatttgaga attagtagta ctgtattagt
      121 gagcttattg tttgggcttg ctttggtttt ggtgaatagt tggtttaatc agccaggcgt
      181 tgaggaagtt gttccacgat caacctatct gatggtaatg attgctttat tttttatcga
      241 tactgttgca tttattttta tgcagttgta tttcatttat gaccgtaggc aattttcaaa
      301 ctgtatactt agtttggctt ttctgagttg tttgatttac tttgttaaaa ccgtcattat
      361 cattcagcaa attattgagg ggcgtttgac aagcagtgtt gtccaaaatg acattgcaat
      421 ctattatttg tttcgtcaga tgagtttgtg catattaata tttctggcat tggtgaataa
      481 agttagtgaa aacacaaaac agcgcaattt attttccaaa aaaatgactt tgtgcataag
      541 tttgtttttt gtttttgggg gggcgatagt tgctcacata ctttctagtc attatgagag
      601 ctacaattta cacattgccg agttaaccaa tgaaaatggt caggtggttt ggaaagcctc
      661 atatgttacc ataatgattt tcatgtggtt aaccttgctt tcggttaatc tatactttaa
      721 tggtttacgc tatgatatct ggaatggagt gacggtgatt gctttttgtg ccgtactgta
      781 caatatctct ctgttattta tgagtaggta tagcgtgtcc acttggtata ttagtcgcac
      841 tattgaagta gtcagtaagt taaccgttat ggtaatattt atgtgccata tattcagtgc
      901 gctacgagta acaaagaaca ttgcacatcg cgatccctta accaatatat ttaacagaaa
      961 ttattttttt aatgaactga cagttcaatc agcatcagcc aaaaaaacgc cttattgcgt
     1021 catgattatg gatatcgacc acttcaaaaa agtcaacgac acctgggggc atccggttgg
     1081 cgatcaggtg ataaaaacag tggtgaatat cattgggaaa agcatacgac cagatgacct
     1141 tttagcgcgc gtcggcggcg aagagtttgg cgtcttgctg acggacatcg atactgaacg
     1201 tgcgaaagct ttagcggaaa ggattcggga aaatgttgag cgtttaactg gcgataatcc
     1261 tgaatacgct ataccacaaa aagtgacgat tagtattggc gctgttgtga ctcaggaaaa
     1321 tgcgttaaac ccgaatgaga tctatcgact ggctgataac gcactttacg aggcgaaaga
     1381 gaccgggcgt aataaggtgg ttgtgaggga tgtggtgaat ttttgtgagt caccataaag
     1441 cggcat
//
LOCUS       DAZFYC010000338         1435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFYC010000338 DAZFYC010000000
VERSION     DAZFYC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.345"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..114
                     /locus_tag="ACHKW5_005521"
     CDS             <1..114
                     /locus_tag="ACHKW5_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO7514650.1"
                     /translation="TLLGERIMDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            136..852
                     /locus_tag="ACHKW5_005522"
     CDS             136..852
                     /locus_tag="ACHKW5_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7514651.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            885..1166
                     /locus_tag="ACHKW5_005523"
     CDS             885..1166
                     /locus_tag="ACHKW5_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7514652.1"
                     /translation="MILRQCAGTRKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            1163..1390
                     /locus_tag="ACHKW5_005524"
     CDS             1163..1390
                     /locus_tag="ACHKW5_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514653.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
BASE COUNT          376 a          292 c          461 g          306 t
ORIGIN      
        1 acgcttcttg gtgagaggat tatggaccgc atgaccatga acggtggtcg gtgggtgacg
       61 tttaactggg atagctggcg tccaaatgtc agcaatcaga gggttgtaaa gtaatttttg
      121 ttggaggacg ttttaatgga aactgtattt gacgcactga aagcactgaa aaaagcctct
      181 tcacaggtag tggcatcgcg ccttggaatc agccgcgaag atgctgtcaa cgaactgtgg
      241 aaactgaagc gccgtggtga agcggataac aagggttcga tgtggtggct gattcaggct
      301 ggtgaaagtg aaccagtgtc accggtaccg aaagtgacag cgcaaatgct gactgaggcg
      361 attgaacatc atggcccaca aacggcggat gagctggcac tgatgttcgg gattacctcc
      421 cgccgggcga attcatcact ggccatggca atcagcaaag ggcgtctgat tcgcgtgaat
      481 cagggcggta aatttcggta ctgcataccg ggcgctgatt taccggcaga gccgaaagcc
      541 gcatccatag cggaaacgga tggtaaagcc tttcctcagc cagcaggtgt tgcgttacca
      601 gtcggggaag cggaaacaca ggaagaaata aaaacggaaa gtgtggcggt cacagtgcag
      661 tcacagccgt cgttcaccag aaagcatccg gatggtctga ttttaccatc gctgcatgtg
      721 gctaaccgcg agctgcgccg ggcaaaaggt caggttcaga agtgggagcg agtctgcgcc
      781 gcgctgcggg agctgaacaa gtgccgggat attctccggg atattaccgc caccagagaa
      841 cagcagcggt gagtgggtgg aagacgtggt gccgggcgga aatcatgata ctccggcagt
      901 gtgcgggaac gaggaaggta aaaagcgttg gcgcacttat cggacgaact gaagcggcag
      961 tgagaacgaa ggcacgggag ctgggcatca gcatgatgtt acgtggtgat tttcacccgt
     1021 cggcaaaata ttctcagcgt gatattgagc tggcgcggca actgcatcag agaggcatgc
     1081 aaagaaggga aattgccaga aaattaggca tgccgctgcg catagtgaat aactacgttt
     1141 atttcgacag gagggtgtct gcgtgaaaat cctgtatcag gattacggcc cggtggggca
     1201 ggtggttatc agcagtactg taatggagtt tcggaagcat aaccgtgtgg tggatgctgt
     1261 gctgttaacc tgtccgggga tatcggcgag tcgtgcaggt gtgtttatta tgaagacgaa
     1321 attatatggc agtaaggcgt ggataaagaa ggcgtatcgt gtagcgttgc aggaggttaa
     1381 cagtgagtaa aattaaagaa atgccggtag ttcgtgacgg atatggctac tggac
//
LOCUS       DAZFYC010000339         1428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFYC010000339 DAZFYC010000000
VERSION     DAZFYC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.346"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..881)
                     /locus_tag="ACHKW5_005525"
     CDS             complement(<1..881)
                     /locus_tag="ACHKW5_005525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO7514654.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIG"
     gene            complement(945..>1428)
                     /locus_tag="ACHKW5_005526"
     CDS             complement(945..>1428)
                     /locus_tag="ACHKW5_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO7514655.1"
                     /translation="RKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPL
                     MAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI
                     "
BASE COUNT          294 a          471 c          353 g          310 t
ORIGIN      
        1 ccaatcccct tatccagttt ctgctccaca cggatcaccg gcgcagaagc caccgcggcc
       61 acattcccgt taccggcctg tttcaccggc tgcgccgtgg cggccttacc ggcttccagt
      121 tcacgcaggc gcttcaggtg cgcatccacc tgacggattt ccgctgcggt gttgtcgtaa
      181 tgctcttcct cctccacatc cagcgtgcgc ccttcctctg cggctttggt catgacctcc
      241 tcaagggagg ctgccagcgc tgcacgcttg ttttcaaaac ttttaatctg ttcgccaata
      301 ttcattatgg tcttttcctt atgaaaaacg gttgttgact gtgccgcagc gccggcagaa
      361 gatgcgattt tcaccaccgg tttccggttg ccggacgcgg cagaaaacgg gcggtcgtaa
      421 gatttaatgg tccggatggt gcattccgca ttcgcgggca cggtgacggc agacacctcc
      481 atcagttccc agcgcagaaa atgcagtccg cctccgtcca gaaaggtgta ttcatgggga
      541 cggaagccca cggacagccc cctgaccagc ccggtcttaa tggcagccca ggcctcatcc
      601 agccgggctg ccatctggga gggcatcccc ggctccggct tcaccagcat tgccgtgatt
      661 tccagccctt ccctgacccg acgcaccgta cactggccta cagggcggga atggtcatgc
      721 tgccagagaa acgggatcgt actgccaaac tcagccccct ccggctccag gatgtcacca
      781 tcccgatccg gagaaggcgt tgacgcaatc ccggtgatca cccgttcatc ctcactgaag
      841 gatttcaccg tcagcaggga acaggcccgt ttaagagtca catcagcctc ctgaaaataa
      901 aaaaaccgcc gcagcggttc atgatggtta cagggtgagc agggttatat gaaaaaaacc
      961 tcatacgctt tctttttcgg ttccggattc agggacatca gggacaccgc attgaagagc
     1021 gccatcagcg ggtcaatttt tccccgtccg ctggcctgtt tggtgataag aatggcgtta
     1081 cctttaggct ccacccgggc attaccgaca caccaggcca tcaggggctg atcaccgtga
     1141 atcagcaccc cttcagccag tttgcgctcg gtggttttga tggccccgcc cagcttccag
     1201 ccctggctta tccccaccac actctcatcg gggatcccgg cttccgccag tgaatccaga
     1261 atctgcccca cacctgacgg gtcaataccg atatgatcca gtaactcagc ctcatgaata
     1321 cgacgcacat actccgccac ttccgccgtg tcatccccga cccgacggac aatcgtcatg
     1381 tctccacagg ccacaaaatc ctgaaaccgg gatgcctcac tcttccgt
//
LOCUS       DAZFYC010000340         1413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFYC010000340 DAZFYC010000000
VERSION     DAZFYC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.347"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1413)
                     /locus_tag="ACHKW5_005527"
     CDS             complement(<1..>1413)
                     /locus_tag="ACHKW5_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7514656.1"
                     /translation="VAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKE
                     AIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFML
                     RLTVAADDGSERLVSTARTAETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIA
                     APAAPSQIELTPGYFQITATPHLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTAL
                     YWIAASINIKPDHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGQITESHLG
                     KELLEKVDLTEDNASRLEEFSKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMED
                     AEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVR"
BASE COUNT          273 a          434 c          361 g          345 t
ORIGIN      
        1 cctgacggta atggtccctg atggccaccg ctccggtgcc cgggagtccc gcggaaaagc
       61 cttacccact gttttgacta tatcgccttc aatctggttg gctgacagtt tccccttaat
      121 ctgacagttc tcattaattg tgacattgtt gagcgtcccg gcgttcgcat tcacactgcc
      181 actgatatct gcatttttag cggtcagctt tccgtccggt gtcagggaaa atgccggagg
      241 attaccgccg ctggtaatgg tgggagccgt caggcgcttc aggaacacgt cgttcatgaa
      301 tatctggttg ccctgcgcca caaacatcgg cgtttcattc ccgtttgccg ggtcaataaa
      361 cgcgatacga ttggcggcaa ccagaaactg gctcagtttg ccttcctccg cgtcctccat
      421 gctgaggcca atacccgcga cataatgttt gccgtctttg gtctgctcaa ttttgacgcc
      481 ccacatggca ttccacttat cgttggcgtc cttccactct ttcgaaaact cctccagtct
      541 gctggcgtta tcctccgtca ggtcgacttt ttccagcagc tccttgccga gatgggattc
      601 ggttatctgg cctttgaaaa aatccagata gccggatgca tcatcactcg gctggccaac
      661 agcctccaca aatgccgatt tgccaacggt gttcacactg cggatgtaaa aataataatc
      721 atggtccggt ttgatattga tactggcggc tatccagtac agcgccgtgc caagatagcg
      781 ggctgtggtt tcaacctgcc tgatatcggt aatccgcgtt tccgagaacc agaactcaaa
      841 ctgtaccgtc gggtcataaa ccgcaagatg cggcgtggcg gttatctgaa aatagcccgg
      901 cgtcagctca atctgtgacg gcgctgccgg tgcggcaatc ctgaacgata ccgatgccgg
      961 atcgccctgc tgcccccacg catttactgc ccggactgtc agcctgtagt tccccggcgc
     1021 cagttgtgtg aagcggtatg tggtttccgc cgtccgggct gtgctgacca gccgctcact
     1081 gccgtcatcc gcggccacgg tcaggcgaag catgaaactc acgcccttca ccaccttcgg
     1141 cgtatcccag cgggccagta cctgatactc cccgctgtct gcggtgactt cggcagtcag
     1201 gtgctgcact gctggcggcg tgacaccatt caccgtgccg ctctggtcgc cgtcaaagtg
     1261 cgccccgtta tccacgatgg cttctttttc cggtacatgc tgcacggcgg tgatagcata
     1321 cgtgccgtca tcgttctcac ggatactcac acagcggaac aggcgctggc gcaacgtcgg
     1381 caacttcagc ccccacacgc tgtattcagc aac
//
LOCUS       DAZFYC010000341         1411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFYC010000341 DAZFYC010000000
VERSION     DAZFYC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.348"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..87)
                     /locus_tag="ACHKW5_005528"
                     /pseudo
     CDS             complement(1..87)
                     /locus_tag="ACHKW5_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            64..>1411
                     /locus_tag="ACHKW5_005529"
     CDS             64..>1411
                     /locus_tag="ACHKW5_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7514657.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSADGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAIL
                     RHAGRVALNAGASSTVSEVRPSSPSGAEATGITTLLSYLASESEGSLKVQGWSASGG"
BASE COUNT          334 a          351 c          435 g          291 t
ORIGIN      
        1 catcctgtaa tttgattccg gttccggctt tttaactctg ttcctgtaca cgggagaaat
       61 tcgatgtcga ttaaacatta tgatgttgtc agggcggcgt cgccgtcaga ccttgcggaa
      121 aagctgacac acaaactgaa ggaggggtgg cagccatttg gcagcccggt ggccatcacg
      181 ccttatactc tgatgcaggc cattgcggcg gaaggtgatg tcaccacacc tgtgttggtg
      241 aagccgtcgg atggagaagg cacagtaatc agcgccacca gagacccgga gtattacttt
      301 gttgtggttc tggcggggca gtcaaacagc atggcatatg gtgaaggcct tccgctgccg
      361 gagacatatg accgtccgga cccgcgtatt aagcagctgg cgcgccgcag tacggtgaca
      421 ccgggcggtg tcgcctgtaa atataacgac atcattccgg cggaccattg tctgcatgat
      481 gtgcaggaca tgagccgcct taaccatccg aaagcggacc tgtcaaaggg gcagtacgga
      541 accgtggggc aggggctgca tatcgccaaa aaactgctgc cgtttatacc ggcgaatgcg
      601 ggcattctgc tggttccgtg ctgtcgtggt ggttcagcgt tcaccaccgg agctgatggc
      661 acatacagtg acgcgagtgg tgcctcggag aattcaaccc gctggggtgt ggacaagccg
      721 ctgtataagg accttatcgg tcgaacaaaa gcagcactga agaagaaccc gaaaaatgtg
      781 ctgtttgccg tggtgtggat gcagggggaa tttgattttg gcggtacgcc ggcaaatcac
      841 gcagcacagt ttggtgcgct ggttgataaa ttccgtgcag acctggcgga tatggcaggt
      901 cagtgcgtcg gtggctctgc tgacggtgtt ccctggatat gcggggacac gacgtatttc
      961 tggaagcaga agaacgaatc ctcgtaccag acggtgtacg gcagctacaa aaacaaaacg
     1021 gaaaaaaata tccatttcgt accgttcatg acggatgaga acggggtgaa tgtgccgacg
     1081 aacaaaccgg aagaagaccc ggacattccg ggtatcggat attacggttc gaaatggcgt
     1141 gacagctcag ccacctggac gtcacaggac agggcgagcc atttcagcgc ctgggcacgc
     1201 cgtgggatta tttccgaccg tctggcaacg gcgattttgc gccatgcggg aagagtggcg
     1261 ctaaacgcgg gggcatcatc gacagtatca gaggtgcgcc cgtcatcgcc ttccggtgca
     1321 gaagccacag gcatcacaac actgctctct taccttgcca gcgagtcaga gggaagcctg
     1381 aaagtacagg gatggtcagc cagtggcggc a
//
LOCUS       DAZFYC010000342         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFYC010000342 DAZFYC010000000
VERSION     DAZFYC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.349"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(147..491)
                     /locus_tag="ACHKW5_005530"
     CDS             complement(147..491)
                     /locus_tag="ACHKW5_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514658.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFNKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(594..773)
                     /locus_tag="ACHKW5_005531"
     CDS             complement(594..773)
                     /locus_tag="ACHKW5_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HFO7514659.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSDDYVCCDCGYNDSKDAFGERGKN
                     DFVKINKEREGNEKS"
     gene            complement(870..>1408)
                     /locus_tag="ACHKW5_005532"
     CDS             complement(870..>1408)
                     /locus_tag="ACHKW5_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO7514660.1"
                     /translation="DMHFLLWDAQRRAGISDAEITAAMEDKLKINMERQWPEPKDGEP
                     RLHIKEPGNSPVIPDGLSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQQKLVHR
                     DVLPFSLPVTPDGWISCSELMPDDGQHVIILCDGAFVLYAQYRDGEFFDIVRNGDEFF
                     ETQSRNVTDWMQLPEPPL"
BASE COUNT          379 a          333 c          290 g          406 t
ORIGIN      
        1 gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca cgcaagaaaa
       61 agccgcattg atgcggcgat ggtaggtctg gatatcattg agtaatgaac aggcctcacc
      121 gagtgtgagg cggtgttatt tcatggttag tccttgcgta gctcgctgat tcttctgtaa
      181 gtctctggtg ctttgtttcc gtgtatcttc atttcagact tcaacagagc aacgagggaa
      241 tcccattcgt tgaggattcc tttgaatgcc ggaacgcgct ttgcaacctt gttgaatgaa
      301 tctctgattt ctggaatctg ctcaacaagt gcaacgcatc gtctgaaatc ggctgcgtca
      361 tggggagcgc cgaagtgatg accatagata ttctttttca gtccacatgc gattgaggca
      421 agagttgcgc tactgatgcc aacatcgcca gtcgattgcc atttcaaaac cttcatagcc
      481 aaatctgaca tttcttgtct ccaataaaaa aaaccgccat caggcggctt ggtgttcttt
      541 cagttcttca attcgaatat tggttacatt gttttcatat atgaataaat aaattagctt
      601 ttttcgttgc cttcgcgttc tttattaatt ttgacaaaat cgtttttacc acgctctcca
      661 aatgcgtctt tagagtcgtt gtatccgcaa tcgcagcaca cataatcatc agaccatcca
      721 cgcattgttt tttcttttgc aatatttcca gaaccgcatt ttggacaaga catatcacta
      781 cctccaaagc atgagagaga tgacaacgta acattgattg gagattaaca atagattgct
      841 gatgtaaaag atatgtataa gcttagctat caaagaggag gttctggtag ttgcatccag
      901 tcggttacat tgcgactctg tgtttcgaaa aattcatcac cattgcggac aatatcgaaa
      961 aactctccgt ctcgatattg cgcataaaga acgaatgcgc catcacataa aataattacg
     1021 tgctgaccat catctggcat tagctcacta cagcttatcc aaccatccgg agttaccgga
     1081 agcgagaacg gcagcacatc tctgtgaaca agtttttgct gtgacaggtt atccagaact
     1141 ttctgtactg ctgcatcacc gaatacacca agcgcatctg ccataactcc tacaacctga
     1201 taagcctcag cgcataccgt ggataaacca tccggaatta ccggagagtt gccgggttct
     1261 ttaatgtgca agcgaggctc accatctttt ggttcaggcc actggcgctc catgttgatc
     1321 ttcaatttat cttccatagc agcggtaatt tcagcatcgc tgatgccagc acggcgctgt
     1381 gcatcccaca acaggaaatg catatcag
//
LOCUS       DAZFYC010000343         1398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFYC010000343 DAZFYC010000000
VERSION     DAZFYC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.350"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..961
                     /gene="nleD"
                     /locus_tag="ACHKW5_005533"
     CDS             263..961
                     /gene="nleD"
                     /locus_tag="ACHKW5_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO7514661.1"
                     /translation="MRPTSLNLALHQSSTSSSMSDAGIESLVKTSSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVIAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRPESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSQGFLQVRLHPLL"
BASE COUNT          418 a          269 c          327 g          384 t
ORIGIN      
        1 atatttgaca gttcaaaggg aaacttcatt ataaaagatg gttaaaatgt tttacatgaa
       61 gcaatattga ttttcggtag tttatataaa acaccacaga cattcaaaat gtcagaggat
      121 gtcagtggtg gatgtacctg gagcggaagt tgtggaagta agttatgtta tatcgcaata
      181 aaccgaatac atctgtgatg ttacattggt ctgagcttca gtgtgtggtg aatgaaactc
      241 gtaacatttt cagaggtcct tgatgcgccc tacgtccctc aacttggcat tacatcagtc
      301 atcaacgtcg agttcaatgt cagatgcagg tatcgagtct cttgtaaaaa catcgagcgt
      361 tcaatggata aaaaacaatc cgcaacttcg tttccagggg actgatcata atatatatca
      421 acagattgaa gcagcgctcg ataagattgg ctctacagag acagggcgtg tactcctgaa
      481 tgctattgaa tcaatatccc gacttaaatc agaaacagtg gtaatacacc tcaattcttc
      541 cagactagga gttattgcac atagagatat agatgctgag aaccatcggg gaactggttc
      601 cgattttcac tgtaatctga atgcagttga atatccctgt ggggagggga ttagcgtggt
      661 ggactttcat gcgactattg tttttcatga gttgctccat gtgttccaca atttaaatgg
      721 ggagcgcctg aaagttgaga gttcccgacc agaatcacaa aaatactctc cacttttact
      781 cgaagaagcc agaactgttg ggttgggggc tttttcagag gaggtgcttt cagaaaataa
      841 attccgcgaa gagattggga tgccccgtag aacatcctac cctcacgact cagctctcat
      901 tcatgatgac aatacagtga gtcagggatt cctacaggta agactgcatc cattgcttta
      961 ggtgtgcttc ttgtggtgaa tatatattgg tgggataaaa ctgagataca taaggtctca
     1021 caatggcttg caaggctttg tgcttctctg taggggcgac atgtttttat ccggtgcgcc
     1081 gtaaaacccc gtccttcagg gcgtggagga tgtcaaataa taaggaatag tggatatgtt
     1141 tgcagcaaag cccgagagca gtcaaagaag tatttctgga ttattcagtc ctaggtcaaa
     1201 tggtgaaaac gcaagtaata aaccgtcaag tggtatggcg aacagaatca aaccagttgt
     1261 aagcgattca caaatcggta gatatacaac tgacagtaat actctgaagg atggattgtg
     1321 tagccaagcc cacataggaa ctcaacctta tcggccaggt gctgactcgc cgctaactgt
     1381 gagatcattc ctacaaat
//
LOCUS       DAZFYC010000344         1389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFYC010000344 DAZFYC010000000
VERSION     DAZFYC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.351"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1389
                     /locus_tag="ACHKW5_005534"
     CDS             <1..>1389
                     /locus_tag="ACHKW5_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7514662.1"
                     /translation="ENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNID
                     GSIHRWAKNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVAL
                     NKYITEYLTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVE
                     REGPFYFLFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNSTYSGSQQNTG
                     ASDTIAQPEPVKVTADEVNKIMQAANISQPDANKLLAVSRGEFVAGISDPNDPKWVKG
                     IETRDSVNQNQQETEQNGQKAEQNSPNALQNEPETKQPEPVAQQEPEKVCTACGQSGG
                     GNCPACGAVMGDATYQETFDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSET
                     GETADPLITMNGHHVITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGP
                     EFSKTIDLETAGG"
BASE COUNT          446 a          334 c          351 g          258 t
ORIGIN      
        1 tgaaaattca acaggaaaaa tcagcccggt cattgctgcc attcatcgcg aatacaagca
       61 gacatggaaa acactggatg acgaactggc ctacgctctc tggcctggtg atgtggatgc
      121 cggaaacatt gacggcagca tccatcgctg ggcaaaaaat gaagttatcg acaacgaccg
      181 cgaagactgg aagcgtatct cggcatcaat gcgcaaacag cctgatgccc ttcgctacga
      241 ccgccagact atttttggcc ttgtccgtga acgtccgatc gacattcaca aagaccctgt
      301 ggcactgaac aaatacatta ctgaatacct gactacaaag ggcgtgtttg aagatgaagg
      361 aagaaatcag agcgcaactg atactctctc gtcgccagta ccagaaactg atgcagtgga
      421 aacggcaatt ccggacaacg aaaaaaccga atgcaaagtg gaagtcgaac catctgtaga
      481 gcgtgaaggg ccgttctact tcctcttcac cgacaaggat ggcgaaaaat acggtcgcgc
      541 aaacaaactt tctggtctgg ataaggcgct ggctgccggg gctactgaaa tcacgaaaga
      601 agaatatttc gcccgcaaaa acagtacata ctcaggttca caacaaaata ctggtgcatc
      661 tgacacgatc gcacaaccag agccggtaaa agttaccgct gacgaagtaa acaaaattat
      721 gcaggcagcc aatatcagcc agcctgacgc caataagttg cttgctgtat cacgtggtga
      781 atttgttgca gggattagcg acccgaatga tccgaaatgg gtgaagggga ttgaaacccg
      841 cgattcagtg aatcagaacc aacaagaaac ggaacagaac ggccagaaag cggaacaaaa
      901 cagcccaaat gcgttacaaa acgagccaga aacgaaacaa cctgagccag tagcgcaaca
      961 ggaaccggaa aaagtctgca ccgcctgcgg tcaaagcggt ggcggcaact gccctgcttg
     1021 tggcgcggtg atgggtgacg caacatacca ggaaacattc gatgaagaga atcaggttga
     1081 agttcaggaa aatgatccga aggaaatgga aggcgctgaa catccacaca aggagaatgc
     1141 tggtagcgct caggatcacg ccagcgatag tgaaactggc gagacggcag atcccttaat
     1201 tacgatgaac ggtcatcacg ttatcacatc caccagcagg acgtgtgacc atctaatgat
     1261 cgaccttgaa accatgggaa aaaatcctga tgccccgatc atctcaatag gtgcaatatt
     1321 tttcgatccg caaaccggag atatggggcc ggaatttagt aagactatcg atctggaaac
     1381 tgctggcgg
//
LOCUS       DAZFYC010000345         1380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFYC010000345 DAZFYC010000000
VERSION     DAZFYC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.352"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..320)
                     /locus_tag="ACHKW5_005535"
     CDS             complement(30..320)
                     /locus_tag="ACHKW5_005535"
                     /inference="COORDINATES: protein motif:HMM:NF013211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO7514663.1"
                     /translation="MNQKRAEEKTRCTEGNDFADWSTEFGKVVWTDNWRPFFVKLESL
                     AAGRAASSVTAWTFSVMPGTLVGILGFAIIMAAVSALVNDKFIEQNNKLIGI"
     gene            668..856
                     /locus_tag="ACHKW5_005536"
     CDS             668..856
                     /locus_tag="ACHKW5_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514664.1"
                     /translation="MKKQSKEVKTSGSEHREIMIEIDASKSTNGTGGGSIIEGSYTVY
                     RCDGGSVPSRPFGPPFCP"
     gene            984..>1380
                     /locus_tag="ACHKW5_005537"
     CDS             984..>1380
                     /locus_tag="ACHKW5_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514665.1"
                     /translation="MSLGDKFTSGYRGINAFNSGVIKLLVARENDWIEEKLSAILTQS
                     PRIDFEPLIASIVFGRTEGKTSLFKNVECIVPGCSFKIDHTHNGASVSEYIDWNAISY
                     KTLPHDPAEILFQSVSEVVNRNQGGYFVVR"
BASE COUNT          408 a          279 c          279 g          414 t
ORIGIN      
        1 tcaggaaaat gaaagggtat cattatatgt cagataccaa taagtttatt gttctgctca
       61 ataaacttat cattaacaag cgcactcaca gcagccataa taattgcaaa acccagaata
      121 cctacaaggg tccccggcat gacggaaaac gtccatgcgg taacagatga agcagctctt
      181 cctgctgcca gtgattcaag ttttacaaaa aatggacgcc agttatctgt ccagacaacc
      241 ttaccaaact cagtgctcca gtcagcaaaa tcgttgcctt cagtacaccg tgttttctct
      301 tcagctctct tctggttgat gtcggcaagc tggcaacaaa aattctcttt ttacgtcaag
      361 aacgtcattt gcatgttttt gctcatttgt tctggcactg acgttatttc tcgccgaatt
      421 taccgcagat tcagcaaagg tgattttatt gtaactgcaa cacctaccag atgacggaaa
      481 acgggtgaac ggattagagt acgatttttc catttcgtga tcttcacctc agaaccccac
      541 cattgatgat gaaaatggtt atcattcgca ttgacacttt gaaataagta gttatatttt
      601 tcatggttat tgagcaattg gtaaccaatg taggttgtag gtgtttcatt ttcattaagg
      661 aatatgtatg aaaaaacaat ctaaagaagt gaaaacctct ggctctgaac atcgtgaaat
      721 tatgatagaa attgatgcct ctaaatcgac gaacggtacc ggtggaggct ccatcattga
      781 agggtcatac actgtctatc gctgcgatgg cggaagtgtc ccctcgcgtc cttttggccc
      841 cccattctgt ccttaaattt aaaggctagt catgattgaa atattcactt ctctcaaacg
      901 agagcaatga atacgataac acaggtatat tcgataaaaa ggacgtcttt tgtggcgtcc
      961 ttaccttaag gagattatta cgaatgtctt tgggggataa atttacatct ggataccgtg
     1021 ggattaatgc cttcaatagt ggcgtgataa aacttcttgt tgccagagaa aatgactgga
     1081 ttgaagaaaa acttagtgcc attttaactc aatcacctcg tattgatttc gaaccactca
     1141 ttgcgagtat agtttttggg cgtactgagg ggaaaacttc attatttaaa aatgtggaat
     1201 gtattgttcc tggttgcagt tttaaaattg accacacgca caatggtgca tccgtaagtg
     1261 aatatattga ctggaatgca atttcatata agacattacc gcacgacccg gcagagattc
     1321 tttttcagtc tgtatctgaa gtcgtcaaca gaaatcaagg cggatacttt gttgtgcgcc
//
LOCUS       DAZFYC010000346         1378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFYC010000346 DAZFYC010000000
VERSION     DAZFYC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.353"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..403
                     /gene="yqeJ"
                     /locus_tag="ACHKW5_005538"
     CDS             <1..403
                     /gene="yqeJ"
                     /locus_tag="ACHKW5_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO7514666.1"
                     /translation="AEKMIYNETCTANWVIFNDQGRANLTIDFMFNKKNKTGTVALSG
                     TWQQGNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIMDQVDDDRLVQLIPDFYVF
                     PEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(436..516)
                     /locus_tag="ACHKW5_005539"
     CDS             complement(436..516)
                     /locus_tag="ACHKW5_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514667.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(552..976)
                     /gene="yqeK"
                     /locus_tag="ACHKW5_005540"
                     /pseudo
     CDS             complement(552..976)
                     /gene="yqeK"
                     /locus_tag="ACHKW5_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1170..>1378
                     /locus_tag="ACHKW5_005541"
     CDS             1170..>1378
                     /locus_tag="ACHKW5_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514668.1"
                     /translation="MKPRNINNSLSLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLIHDLSIKIADISEE"
BASE COUNT          511 a          224 c          220 g          423 t
ORIGIN      
        1 tgcagaaaaa atgatatata acgaaacatg cactgcaaat tgggtaattt ttaatgacca
       61 gggacgagca aatcttacca tcgactttat gtttaacaaa aaaaataaaa caggaactgt
      121 tgcgttgagt ggaacctggc aacaaggtaa cagagaaagt aaatcaatac ggcgaaatat
      181 tgaatacaca tggattgaaa actatgacac agcccattta acatcaaaga aagttaataa
      241 gtttgagatt atggatcaag ttgatgatga tagacttgta caacttattc ctgattttta
      301 tgtctttcca gaaaaaagtg taagctataa tatactaaag caaggtaagc atgcttttat
      361 tttgagcatt ggtaacagag caataatgca ttgtgcaagg taaactaaaa tagataacaa
      421 aaagatggaa cctcgttaat cataaaacaa taaaattaaa tattctcgca gtatatggca
      481 gtctaaagca tcaaagtttt gatcaacatc tttcatttta gacatctcct tgcaatgtaa
      541 aatatcatgt atcaacaaca tctggtgcaa atccatgagt cgaactcgac attcatctta
      601 gctcgattat tattccttcg tacagtcgat gtaaacaata cagaaagagg attattaata
      661 accagtttac tcagtgaatc aatagaggaa aggattaacg tttctttaaa agaattaatt
      721 tcatcatctg ttaaactaaa ctcatcattg acagatcgtg agatataact gtttttaact
      781 ttactcttta cgttgacttt attgacagaa ttaacattca catatcttga atttaatgtc
      841 catgatgttg tttgcgtgag aaccttctca gcattaatga attttttaac ggaatccttg
      901 gtttcttttt cgagtccgaa tttacaatat catgcatata gaccatttca tgaatctgcg
      961 aaaactcaat gtccattgta tacctcacat ttttaccgtg actcgatgtt actgttcaat
     1021 aatcaccttc catcaatact aaaattaata cccctaatgt gccgataaca aatatagtca
     1081 ttctacgtaa cgtctccata aggtgatatt tgacattatc agaagctgcg aattcggatt
     1141 ttgctctaat caaatgagag aaatgtaaaa tgaagccacg aaatattaat aatagcctat
     1201 cactgcaacc attagttcct gatcaggaga acaaaaataa gaaaaatgaa gagaaatccg
     1261 ttaatccagt taaaatcaca atggggtctg gtttaaatta tattgaacaa gaatctcttg
     1321 gaggaaaata tctaatacat gatttgtcaa taaagatagc ggatatttct gaagagat
//
LOCUS       DAZFYC010000347         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFYC010000347 DAZFYC010000000
VERSION     DAZFYC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.354"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..490)
                     /locus_tag="ACHKW5_005542"
     CDS             complement(<1..490)
                     /locus_tag="ACHKW5_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514669.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRN
                     IDL"
     gene            complement(512..1051)
                     /gene="yhaB"
                     /locus_tag="ACHKW5_005543"
     CDS             complement(512..1051)
                     /gene="yhaB"
                     /locus_tag="ACHKW5_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO7514670.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1307..>1372)
                     /locus_tag="ACHKW5_005544"
     CDS             complement(1307..>1372)
                     /locus_tag="ACHKW5_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO7514671.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          425 a          244 c          253 g          450 t
ORIGIN      
        1 ctaaatcgat attccgtaag tccagagagg atagatcaac tatacctcca gtcccacaac
       61 taaaaactaa gttcaacctc ctgatttcat tgtaaatgtc tttcaatggg aaattaacat
      121 tgatattgcc tgtggatatt tcctggtttt ttaacggttc attaattgta agttcacagg
      181 tattgtctga tcgttgaata attagggcgt tataaccaaa aaaatccata cgaagagacg
      241 aatcattata aataattaca ttttgtaagt acgctaattc tttttgaaaa tcaccagtga
      301 taatgcattt tccacctggt tgtaacgcgt taagtaaagt gaccatttga atagaggtct
      361 gttttagttt ttctatacct ggtgctagtg cttcgaggtt attaactatg gggctttcag
      421 gaagatcatt catttttcga cggactaaat cactactaat atcattacca attgatgaaa
      481 cagggaacat tattttatcc tttataaata atcaataaaa atcattatta aaatgatcgg
      541 gtgaaaaaaa gagaatttgc caacaatctg aagtaatgct ggttttatta aagtgcgcag
      601 gaggtgttaa gatggcttct tttcgggtga attttgcaca atacttagaa atcgtctcct
      661 taatacattc cagctctttt ttattgacgg ctgagcaagt acattgttca ccaaaaggtg
      721 caaaagtgcc tacatctaat gtttgtatga tataatcgtc atgttctcct gtagaaatat
      781 ctatgatgaa tggactcata ttttccggtt ggattcggat catgacagaa ggaacatcgc
      841 cttgatagaa attcaatgca gtaattgcag ataatactct ccgcagttca ttacttatac
      901 tgtaaatttt attaacgtca ttggtggaga caatttctgc aaactttctt tttaccgtcc
      961 aataatcaat atttctattg tgattgttaa ccactgcgtg cattggcggg attgaagggt
     1021 gaaaaatatg tgcaattggg aagcctttca ttgatgcctg ccttgtcgtc atgacaaaca
     1081 ataatagatg cttattttcc tcgcagggtt ctttcgcact atcaaaaaac gctcatttac
     1141 ccggcgttac agtaaacgaa gcaacattct gtggcctacg acgatcaaaa gctctgtgaa
     1201 aatttccctt gttgtctttg tctgacctta tcgcggcgat cagcaattat tcgttttgtg
     1261 atcctcaaca caaaatgcta ttcgtcgcct atgaggggag aagaatttat ggaaaacgat
     1321 tgcattcaca aaatgacgtc ctgtcagaag agaaaaagtt gacgttaatc aa
//
LOCUS       DAZFYC010000348         1327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFYC010000348 DAZFYC010000000
VERSION     DAZFYC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.355"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1327
                     /locus_tag="ACHKW5_005545"
     CDS             <1..>1327
                     /locus_tag="ACHKW5_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7514672.1"
                     /translation="DGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALKKNPKNVLF
                     AVVWMQGEFDFGGTPANHAAQFGAQVDKFRADLADMAGQCVGGSADGVPWICGDTTYF
                     WKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSK
                     WRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHAGRVALNAGASSIVSEVRPSS
                     PSGAEATGVTTLLSYLASESEGSLKVQGWSASGGRAEVVSDAAGTGGKAVKLTKEAGK
                     SSWVLEYAAGNGAALLQKGGQIRCRFKVSGVLAANQYVMAFYWPVSSLPQGVALTGDG
                     GNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     TPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITKGATYPVLIDSIAVEVN"
BASE COUNT          328 a          314 c          423 g          262 t
ORIGIN      
        1 gatggcacat acagtgacgc gagtggtgcc tcggagaatt caacccgctg gggtgtggac
       61 aagccgctgt ataaggacct tatcggtcga acaaaagcag cactgaagaa gaatccgaaa
      121 aatgtgctgt ttgccgtggt gtggatgcag ggggaatttg attttggcgg tacgccggca
      181 aatcacgcag cacagtttgg tgcgcaggtt gataaattcc gtgcagacct ggcggatatg
      241 gcaggtcagt gcgtcggtgg ctctgctgac ggtgttccct ggatatgcgg ggacacgacg
      301 tatttctgga agcagaagaa cgaatcctcg taccagacgg tgtacggcag ctacaaaaac
      361 aaaacggaaa agaatatcca tttcgtaccg ttcatgacgg atgagaatgg ggtgaatgtg
      421 ccgacgaaca aaccggaaga agacccggac attccgggta tcggatatta cggttcgaaa
      481 tggcgtgaca gctcagccac ctggacgtca caggacaggg cgagccattt cagcgcctgg
      541 gcacgccgcg ggattatttc cgaccgtctg gcaacggcga ttttgcgcca tgcgggaaga
      601 gtggcgctaa acgcgggggc atcatcgata gtatcagagg tgcgcccgtc atcgccttcc
      661 ggtgcagaag ccacaggcgt cacaacactg ctctcttacc ttgccagcga gtcagaggga
      721 agcctgaaag tacagggatg gtcagccagt ggcggcaggg cagaagtggt cagcgatgcg
      781 gcgggaaccg gaggtaaggc agtgaagctg accaaggaag ccggtaaaag cagctgggtg
      841 ctggagtacg ccgcgggcaa cggtgcggct ctgttacaga aaggggggca gattcgctgc
      901 cgctttaagg tttcgggagt gctggctgcg aaccagtatg ttatggcgtt ttactggccg
      961 gtatcttcac tgccacaggg cgttgccctg accggagacg gggggaataa cctgctggca
     1021 gcgttctaca tccagacaga tgcaaaagac ctgaatgtga tgtaccacaa tgcgaaagtg
     1081 gcgacaaaca acctgaaact gggaaccttt ggcgcatttg ataacgaatg gcatacgctg
     1141 gccttccgct ttgccgggaa taacagcctt caggtgacgc cggttattga tggtcaggat
     1201 ggcacaccgt tcacgctgac gcagtcaccg gtcagtgcat ttgcggcgga taaactgcat
     1261 gtgacagaca ttaccaaagg tgcgacttac ccggtgctga ttgacagcat tgcggtggaa
     1321 gtgaaca
//
LOCUS       DAZFYC010000349         1319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFYC010000349 DAZFYC010000000
VERSION     DAZFYC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.356"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..253
                     /gene="excA"
                     /locus_tag="ACHKW5_005546"
     CDS             <1..253
                     /gene="excA"
                     /locus_tag="ACHKW5_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO7514673.1"
                     /translation="IGLDIHNFTRTVAEDGKLEIHTTYVSLPMIPLEISGISARTLAN
                     TMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(567..719)
                     /locus_tag="ACHKW5_005547"
     CDS             complement(567..719)
                     /locus_tag="ACHKW5_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7514674.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            1052..1294
                     /locus_tag="ACHKW5_005548"
     CDS             1052..1294
                     /locus_tag="ACHKW5_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514675.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
BASE COUNT          370 a          327 c          294 g          328 t
ORIGIN      
        1 gattggtctg gatattcaca acttcacccg aacagttgct gaagacggca agctggaaat
       61 ccacaccacc tatgtgagtc tgccgatgat ccctcttgaa atctccggca tctcggcaag
      121 gacgctggcc aacaccatgc acaccatggc tgcacgtgga tatgagtaca aagagcgttt
      181 cccgcagatg atccggtatc gggttaaaga gtgggaaaaa gttgccggtg tgcctgttgc
      241 agaagtattt taaggtagcg accaactgtt agacgataat ccgtcagccg gttttctctg
      301 aaccggcttt ttcttttcag gcagaatgcc tgtctgccgt ccctgtatca gactgtaatt
      361 agcacaatac ggcaatttgc cgttcagtat attcaggaag tacaaatcat gccatctgca
      421 acacaaactg cccgactgga agcaaggatc agcacagaac tgcatgctat gctgaaacgc
      481 gctgcagaaa tccaggtaaa aaagggcgca ttgaggtttg ctttcacaac aatccggcgg
      541 cagatttcct gccgccgtgt tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca
      601 agcactgtgt ttccctgctc gacacggaat ccgcaaagcg aatccctcac catccagaca
      661 aaacacagta tcgtcacaca gacgacaatt aacattgtta aaaacgttcg ttgtggcatc
      721 gcagcccctc cattgccttt cggccgtcat cgggctacta taatgttgtc taggcattac
      781 agtgggccct gggttgattg ataaaaaaat cgcctggggc tttctgctgc atgccgtcca
      841 caaaacgctt tcagcataca gcctcaagcg cccgccgcta ttctaatcga ttttcagtct
      901 gccacaatcg ttttccacct aatgacatgt tatgcacaac agtattttta cgggaaacta
      961 cacggcaagg attcccggaa cacgttcatt atttgatggt tacagacatc atgttaaatc
     1021 cggaagtaat aatgacctga aaggaaatac tatgagtaac gaatatcaaa gcaccagtat
     1081 aaaccctgat gtttttgaac aatttcttac cctcctgaat acacactgta aatcatcaga
     1141 agctgcactt gccatgctga gtacactctg ctacgaactg atgaggagga gtgggatcca
     1201 ttcactggac agcctgatgc tcaacggaga aggtatattg atacaattac agggggctga
     1261 tattcgcgaa aaaacaggtc agcatattca ctgatccagg tatgggctga aaacagtgt
//
LOCUS       DAZFYC010000350         1304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFYC010000350 DAZFYC010000000
VERSION     DAZFYC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.357"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..413
                     /locus_tag="ACHKW5_005549"
     CDS             18..413
                     /locus_tag="ACHKW5_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7514676.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDTAGQDTPLSQENVLTGHENEVVSAQTDTVTDTAALVTVVALVTLHTVALHATRDEP
                     VAFVLPGTAFRVSASVAAEMTEHGLARMQ"
     gene            425..778
                     /locus_tag="ACHKW5_005550"
     CDS             425..778
                     /locus_tag="ACHKW5_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7514677.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGMSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            793..>1304
                     /locus_tag="ACHKW5_005551"
     CDS             793..>1304
                     /locus_tag="ACHKW5_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514678.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVKRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENIDRIRRERLP
                     GELAYALKQQL"
BASE COUNT          316 a          323 c          397 g          268 t
ORIGIN      
        1 tctctgtgga gaagtccatg acgaaagatg aactgattgc ccgtctccgc tcgctgggtg
       61 aacaactgaa ccgtgatgtc agcctgacgg ggacgaaaga agaactggcg ctccgtgtgg
      121 cagagctgga agaagagctt gatgacacgg atgacactgc cggtcaggac acccctctca
      181 gccaggaaaa tgtgctgacc gggcatgaaa atgaggtggt atcagcacag acggataccg
      241 tgactgatac ggctgctctg gtcacggtcg tggcactggt gacgctgcac actgttgcac
      301 ttcacgccac gcgggatgaa ccagtggcat tcgtactgcc gggaacggcg ttccgtgtct
      361 ctgccagtgt ggcagccgaa atgacagaac atggcctggc cagaatgcaa taacgggagg
      421 cgctgtggct gatttcgata acctgttcga tgctgccatt gcccgcgccg atgaaacgat
      481 acgcgggtac atgggaatgt cagccaccat gacatccggt gagcagtcag gtgctgtgat
      541 acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga
      601 aggctccagc ccgtccctgt ttgtccggac tgatgatgtg cggcaactgc ggcgtggtga
      661 cacgctgacc attaacggcg agatgttctg ggtggaccgt gtttctccgg atgacggagg
      721 gagttgttat ctctggctca accgtgggca accaccggca gttaaccggc gacgataaac
      781 gcagggtgaa ttatggcgat aaaagggctt gatcaggcga ttgaaaatct gagccgggtt
      841 cgtaaaaacg ccattccggc ggcttcagca atggccatta accgcgtggc cacaacggcg
      901 attaatcagt cttcatcaca ggttgcccgg gagacaaagg tacgccggaa actggtaaag
      961 gaacggtcca gactgaaacg ggccacggtc agaaatccga atgccagaat tatcgttaaa
     1021 cgcggtgatc tccctgtgat taagctgggg atcaggatgc tgggccgtcg tccggacagc
     1081 atacttaaag ctggtcagca tcggtatcag cgggcattta tccagcgatt aaaaaatggt
     1141 cgctggcatg tcatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     1201 atcccgatgg cggccccact gaaacaggcg tttgatgaga atattgaccg tatccggcgt
     1261 gaacgtctgc ccggagaact ggcatacgcg ctgaaacaac aact
//
LOCUS       DAZFYC010000351         1303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFYC010000351 DAZFYC010000000
VERSION     DAZFYC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.358"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..589
                     /locus_tag="ACHKW5_005552"
     CDS             <1..589
                     /locus_tag="ACHKW5_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO7514679.1"
                     /translation="YITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            561..944
                     /gene="yhhH"
                     /locus_tag="ACHKW5_005553"
     CDS             561..944
                     /gene="yhhH"
                     /locus_tag="ACHKW5_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO7514680.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1049..>1303
                     /locus_tag="ACHKW5_005554"
     CDS             1049..>1303
                     /locus_tag="ACHKW5_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO7514681.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICK"
BASE COUNT          472 a          187 c          287 g          357 t
ORIGIN      
        1 atatatcact caggatccga ttggactgaa ggggggatgg aacctgtatg gatatcaatt
       61 gaatccgata tcagacatcg accccctggg tttatctatg tgggaggatg caaaatcggg
      121 ggcatgtact aatggtcttt gcggcacact atccgctatg ataggtccag ataaatttga
      181 ttctatagat agcaccgcat atgacgcctt aaataaaata aatagccaat ctatttgcga
      241 agataaagag ttcgctggtt taatatgtaa ggataatagt ggcagatatt tctcaacagc
      301 acctaaccga ggagaaagaa aaggatcata tccattcaat agcccttgcc ctaatggtac
      361 tgagaaagta tcagcttatc atactcatgg tgcagatagt catggagaat attgggacga
      421 aatattttca ggtaaagatg agaaaatagt taaaagtaaa gataacaata tcaagtcatt
      481 ttatttaggt acgcccagtg gtaattttaa agcaatagat aaccacggga aggaaataac
      541 aaacagaaaa ggattaccta atgtctgcag agttcatggt aatatgtaaa aaaatattgt
      601 ttaggaactg tgtcattgta tctttgtttg tttttacata caacacatgg gcgcagtgta
      661 ataataatat taaaataatg cgcaagtatg aaagtgaagg taaatatacc gttagaaatt
      721 tggttaaaaa taaagctata gcattggaat tagctgagat atatgttaag aatcgttatg
      781 gacaggatgc cgcagaagaa gaaaaaccat acgaaatcac tgagttaaca acaagttggg
      841 ttgttgaagg taccattcac tcagaccaaa ttgctggtgg ggtttttatt atagaaatag
      901 gcaaaaatga tgggagaatt ctgaattttg gccacggaaa ataagtatgt aaggataatc
      961 aaacatcgtg tgcgctgatg gcagagtagg tggaggactc cagacagtca aacgatagaa
     1021 aaagatagcc tttatggagg ttcctgcaat gtcaaataca taccagaaaa gaaaggcaag
     1081 taaagagtat ggtttatata atcaatgtaa gaaactaaat gatgatgaat tatttcgctt
     1141 acttgatgat cacaattcct tgaaaaggat ttcatctgcc agagtattac agttaagagg
     1201 tgggcaagac gctgttagat tggcaattga gttctgctct gataaaaatt atatccgtag
     1261 agatatcgga gcatttatac tcgggcaaat aaaaatttgc aaa
//
LOCUS       DAZFYC010000352         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFYC010000352 DAZFYC010000000
VERSION     DAZFYC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.359"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..831
                     /locus_tag="ACHKW5_005555"
     CDS             <1..831
                     /locus_tag="ACHKW5_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7514682.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGK
                     KWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
     gene            883..1266
                     /gene="gpFI"
                     /locus_tag="ACHKW5_005556"
     CDS             883..1266
                     /gene="gpFI"
                     /locus_tag="ACHKW5_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7514683.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRQCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
BASE COUNT          295 a          298 c          410 g          288 t
ORIGIN      
        1 ggtggtgaaa cccgcgtgtt acgtccgggc tacgtcaagc cgaaacacga atttaattac
       61 cagcaggcgg ttgagcgtct tcccggtgaa gatccggctc aactgaacga cccggcctac
      121 cgtcgtctgc gtatcatcac cgataacctc aaacaggaag agcatgccat tgtccaggtg
      181 gaagaaatgc aggcggtgaa tgccgtgctg tatggcaaat acaccatgga aggggatcag
      241 tttgatactg tcgaggtgga ttttggacgc tctgaaggaa ataacattga gcaggccgac
      301 ggtaaaaaat ggtctgagca ggaccgtgat acgtttgatc cgacgcatga tattgacctc
      361 tactgcgatc aggccagcgg tcttgtgaat attgccatta tggacggtac tgtctggcgt
      421 ctgctgaatg gttttaagct gttccgcgaa aaactggata cccgtcgcgg ctcaaattca
      481 caactcgaaa cggcagtgaa agacctgggg gcggtggtgt ctttcaaagg gtattacggc
      541 gatctggcca ttgtggtggc gaaaacgtct tatgtggcag aggacggtac cgaaaaacgt
      601 tatctgccgg agggcacgct ggtcctggga aatacggctg cagatgggat ccgttgttac
      661 ggtgccattc aggatgcgca ggcgttgtcc gaaggtgtgg tggcttcttc ccgttacccg
      721 aaacactggc tgaccgtggg cgatccggcc cgtgaattta ccatgacgca gtccgcaccg
      781 ctgatggtgc tgccggatcc ggatgagttt gtggtggtgc aggtgaaata atccgtgagc
      841 gggggcgaaa tgcccccgtg tcttttttca cagggggctg atatggcaac aaaagaagaa
      901 aatcagaaac gtcttcgtca actggctggc ctgctggggc gcgaggcgga tatgtcgggg
      961 agtgctgcgg atattgcgca acgtgtgtct gagtgggaag aggagcttgc tgcttcccgg
     1021 gagggcatta tgcctggtga tgagagcggg cctgagcaaa atcacacaga cgatggtgag
     1081 cagttgcaca acactgatgc tacggatgat gttaaagcgg tccgtgtgcg gcaatgcctg
     1141 catgtgatgg ggtattgccc ggagacaggc cgtcccgttg aactgacgta ccggggcatg
     1201 cgtgttctgg tgccatcacc actggcgaca gccatgatac agcacggaac ggctgagcat
     1261 gcgtgatttt cagaatgcct ttgatgcagc c
//
LOCUS       DAZFYC010000353         1280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFYC010000353 DAZFYC010000000
VERSION     DAZFYC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.360"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            97..1280
                     /locus_tag="ACHKW5_005557"
                     /pseudo
     CDS             97..1280
                     /locus_tag="ACHKW5_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          372 a          312 c          337 g          259 t
ORIGIN      
        1 atttataaac aataatatat tatcatatct tttaaaaacg gctcactaag aaccttgagg
       61 tggcacacta aatttagcta cctgaacaga ggtgatatgc tcacctcaga acaacacagg
      121 tgccataatg aaaaaagaaa tttcagcgca gagtttaaac gcgaatccgc tcaactggtc
      181 gttgaccaga aatacaccgt ggcagatgca gccagcgcta tggatgtcgg cctttccaca
      241 atgacgcgat gggtgaaaca attacgtgat gagcggcagg gaaaaacacc aaaagcctcc
      301 cccattaccc cggaacaaat tgaaatccgt gagctcagga aaaagctaca acgtattgaa
      361 atggaaaatg aaatattaaa aaggctaccg cgctcttgat gtcagactcc ctgaacagtt
      421 ttcgataatc gggaaactca gggcgcgtta tcctgtggcc actctctgcc atgtgttcgg
      481 ggtccatcgc agcagctaca aatactggaa aaaccgtcct gaaaaaccag acggcagacg
      541 ggctgtatta cgcagccagg tacttgaact gcatggcatc agccacggct ctgccggagc
      601 aagaagcatc gccacaatgg caacccagag aggctaccag atggggcgct ggcttgctgg
      661 cagactcatg aaagagctgg ggctggtcag ttgccagcag ccgactcacc ggtataagcg
      721 tggcggtcat gagcacgttg ctatcccgaa tcatcttgag cgacagttcg ccgtaacgga
      781 accaaatcag gtgtggtgcg gtgatgtgac ctatatctgg acgggtaagc gctgggcgta
      841 cctcgccgtt gttctcgacc tgttcgcaag aaaaccagtg ggctgggcca tctcgttctc
      901 gccggacagc aggcttacca tgaaagcact ggaaatggca tgggaaaccc gtggtaagcc
      961 cgtcggggtg atgttccaag cgatcaaggc agtcattata cgagcaggca gttccggcag
     1021 ttactgtggc gataccagat cagacagagt atgagccggc gtggaaactg ctgggataac
     1081 agcccaatgg agcgcttctt caggagtctg aagaactaat gggtgccagc gacgggctat
     1141 gtaagcttca gcgatgcagc tcacgcaata acggactata tcgttggata ttacagcgca
     1201 ctaagaccgc acgaatataa tggtgggtta ccaccaaacg aatcagaaaa ccgatactgg
     1261 aaaaactcta acgcggaggc
//
LOCUS       DAZFYC010000354         1280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFYC010000354 DAZFYC010000000
VERSION     DAZFYC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.361"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1280
                     /locus_tag="ACHKW5_005558"
     CDS             <1..>1280
                     /locus_tag="ACHKW5_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7514684.1"
                     /translation="PVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSILVNKTPLTD
                     TDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTRTITSANIDRL
                     RVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTTSQFLASVILD
                     NLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFG
                     GQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYG
                     MGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCS
                     AMRCMPVWNGQTLTFVQDRPSDVVWPYTNCDVVVDDNGVGFRYSFSALKDRHTAVEVN
                     YTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFG"
BASE COUNT          291 a          352 c          407 g          230 t
ORIGIN      
        1 ccggtagagg caaaggacaa tcttaagtcc acgcagatga tgagtgtgat tgatgccatt
       61 ggtgaagggc cgattgaagg tccggtgaag gggctgcaga gtatcctggt gaacaaaacc
      121 ccactgacgg acacggacgg caatcccgtg atacacggtg tgaccgcggt ctggcgcgcc
      181 ggggagcagg agcagacacc gccggaaggt tttgagtcat ccggctctga aaccgcactg
      241 ggcgtggaag tgacgaaggc aaagccggtg acgcgcacca ttacgtccgc gaacattgac
      301 cgcctgcggg tcaccttcgg ggtgcagtca ctggtgcaga ccacctcaca gggtgaccgt
      361 aacccggcat ccgtccgcct gctgattcag ctgcagcgta acggtaactg ggtgacggaa
      421 aaggatgtca ccattaacgg caagaccacc tcacagttcc tcgcttcggt gattctggat
      481 aatctgcctc cccgcccctt taacatccgg atggtcaggg agacggcgga cagcaccacg
      541 gaccagctgc agaataagac gctctggtcg tcatacaccg aaatcatcga tgtgaaacag
      601 tgctacccga acacggccat tgtggggctg caggtggatg cggagcagtt cggcggccag
      661 cagatgacgg tgaactacca tatccgcggt cgcattattc aggtgccgtc aaactatgac
      721 ccggaaaaac gcacgtacag cggcatctgg gacggcagtc tgaaaccggc atacagcaac
      781 aaccctgcct ggtgcctgtg ggacatgctg actcacccgc gctacggcat gggaaaacgt
      841 ctgggggcgg cagacgtgga caaatgggcg ctgtatgcca ttgcgcagta ctgcgaccag
      901 acggtcccgg atggtttcgg gggcacagag ccgcggatga cctttaatgc gtacctgtca
      961 caacagcgta aggcgtggga cgttctcagt gatttctgct cggcgatgcg atgtatgccg
     1021 gtatggaacg gccagacgct gacgttcgtt caggaccgcc cgtcggatgt ggtgtggccg
     1081 tacaccaact gcgatgtggt ggtggatgat aacggcgtgg ggtttcgcta cagcttcagc
     1141 gccctgaagg accgccacac ggcggtggag gtgaattaca ccgacccgca gaacggctgg
     1201 cagacctcca cggaactggt ggaagacccg gaagccatac tgcgctacgg acgcaacctg
     1261 ctgaagatgg atgcgttcgg
//
LOCUS       DAZFYC010000355         1276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFYC010000355 DAZFYC010000000
VERSION     DAZFYC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.362"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(469..1101)
                     /locus_tag="ACHKW5_005559"
     CDS             complement(469..1101)
                     /locus_tag="ACHKW5_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO7514685.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          343 a          265 c          263 g          405 t
ORIGIN      
        1 atttaaggcg taatatcttc tataaataac ccgcaaaata tcgtcgtagc tgcactcgat
       61 atttttaaaa tcctgtatca agtgctgaaa atcatttcat cgccacttcc aattcctttc
      121 tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc gtggtaatca
      181 gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc ggtgatttac
      241 aagtaagggt atgattacgc tttttaaagc gtaccataaa acataacata ataaatgatt
      301 tgtgatgaca cgggattatt gggatttact ttgaggaaga taaaatgcgt gtttatagaa
      361 tcgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt
      421 cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt agttcacaaa
      481 cagtgcttcg ctgtcgacga tgattccatc ttccgcacct ttaagcgcca ggtttgcctc
      541 gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac gagtctgttt
      601 aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa gttcttccat
      661 tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg cggcttcttt
      721 ttcgggagtt gctacataac tctcagaaat tccgacagat gcattaatga caatatattg
      781 gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt cggtactgtc
      841 atctgacagc aaagcggaag cttcattggt cagttctttt gccttttctg gatcgccatg
      901 aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt gttcagagat
      961 acgctctgct gctgccgttt tctgggtttg ctgaacttgc tgagtttgct gttgtgccgc
     1021 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
     1081 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
     1141 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
     1201 cttacagttg tagttattat cgcaagaagt attttagtct tacaattgta ggaatctgct
     1261 tcctgtcgca gagaac
//
LOCUS       DAZFYC010000356         1275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFYC010000356 DAZFYC010000000
VERSION     DAZFYC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.363"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..945
                     /locus_tag="ACHKW5_005560"
     CDS             <1..945
                     /locus_tag="ACHKW5_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
                     /protein_id="HFO7514686.1"
                     /translation="TLSNNNYKSLEHLQTRAENYLRHRKAEEKILRSTVEGLTNPESP
                     VFKQAAWMGHLERGLWKTETRWNGNDRDQLGKEALGSEEPKPGSPFYGSRGLKLSDSA
                     RSAFSMMLCGSAGPFTKEQTLSGFELAQTGQVLAGRLKIQERMTFRANNRVDAQRNGT
                     HSTRTQTGMDLSQDIGTIMRDKAGLPVMSGTSGSSSDATLATRYAAEHFGKTWAAPGL
                     SQAEGCKAISDLSHHYFRAEGSSPPQSMATGINKVRCDAGMEEKHVNALDIFTHSYPE
                     IYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
BASE COUNT          381 a          289 c          300 g          305 t
ORIGIN      
        1 acactttcca acaataatta caaatcatta gagcatctgc aaacacgagc agagaattat
       61 ctgagacatc gcaaagcaga agagaagatt ctgcgttcga ccgttgaggg actaactaat
      121 ccagaatctc ctgtattcaa acaagcagca tggatggggc atctggagcg tggcctttgg
      181 aaaacggaaa cacgctggaa tggaaacgat cgtgatcagc taggcaaaga ggctcttggt
      241 tcagaagaac caaaacctgg ttcaccattt tacggtagtc gtggactaaa attatctgat
      301 agtgcccgtt ctgccttttc aatgatgctg tgtggttctg caggaccatt cacgaaggag
      361 caaacactct ccggcttcga gctcgcacag actggccaag tgttggctgg aaggctaaaa
      421 attcaggagc gaatgacatt ccgcgcaaac aatcgcgttg atgctcagcg taacggtaca
      481 cactccaccc gcacacaaac aggcatggat ctttctcaag atatcggcac aatcatgcgt
      541 gataaagctg ggctaccggt catgagcgga acctctggct cctcctctga tgccactctt
      601 gcaacacgtt acgctgccga gcattttggt aaaacatggg ccgcgcctgg cttgagccaa
      661 gcagaaggat gcaaagctat ctcggatctg tcacatcact attttcgtgc agaaggctcc
      721 agtcctccac aatcaatggc aacgggaata aataaggttc gttgtgatgc aggaatggaa
      781 gagaaacatg taaatgcctt ggatatattc acacatagct atccagaaat ttatgctggg
      841 gttgcattaa cgattgctgg ggctggcggt aatgatgagc aggcaatgta taatgttacg
      901 caagaagccg ctcgaattct gcacgaagct gaaaccaaag attgaaaatg ttctaagtga
      961 agaagcccgc cccagaagaa tataatccac attccaggca ttattgatga attttacaat
     1021 caatgcgaaa aaactggcat tgattgtata ctgattacag attctcctga aggagagtct
     1081 gccctgtccc ttctgccagg aagtgttacg caacgatatc tcaccaatct ttcgcaccac
     1141 tattaatcaa tgagttagag gtgattagtg acctgagaca gagcattagc gcaaggtgat
     1201 tttttgtccg taagcgtaaa ccgatcgccg tatgtagcca ttagacaaga attggtaact
     1261 tagacgccca tctga
//
LOCUS       DAZFYC010000357         1271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFYC010000357 DAZFYC010000000
VERSION     DAZFYC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.364"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..147)
                     /locus_tag="ACHKW5_005561"
     CDS             complement(<1..147)
                     /locus_tag="ACHKW5_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7514687.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVM"
     gene            complement(372..926)
                     /locus_tag="ACHKW5_005562"
     CDS             complement(372..926)
                     /locus_tag="ACHKW5_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO7514688.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(935..>1271)
                     /locus_tag="ACHKW5_005563"
     CDS             complement(935..>1271)
                     /locus_tag="ACHKW5_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7514689.1"
                     /translation="YPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRLKLNLSG
                     RLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQLVSGGRLG
                     VKIYKIESE"
BASE COUNT          306 a          355 c          251 g          359 t
ORIGIN      
        1 cataaccagc tgggtcagta ttaactggca gcgttcgcgt gaaaggtaag tattctgcgc
       61 aatctccccg actgtcgccg gttcggtaac gcttaattca ttaaacacca ctctggcggt
      121 ttctgtcata tcctgctgtt ttagcatgtc tttttccctt ttccggttaa cgtgacatac
      181 caataactct tgtcgaaaaa gccagcaagt tgaaagaccg gtattagcaa ccaccagcgc
      241 gtttaacgcc ccgtgccgtt tttcagtcat aaaaaaaccc gcaaaaagcg ggctctttca
      301 aatgtccatg tctgctattc gcctcgcggt acagctttgc gaagcttacc ggaattgaag
      361 cagtttttac gtcaaaaagc aataactttt ttctctatac caaaagccat aaccattggt
      421 ttgtacaaaa taaattctgc cacctttagc caatgctcaa tgcgtctttc acaggttctt
      481 aaactccatt ccggatgtgc atcattcagc aattcagcca ttttgcgctt agtcatcccc
      541 cgccccacat aacgctgact cagaacattg agcagcccgg gataacctgc cagtacttca
      601 ccaataaccc tgtcgattat taacgcctct gaatcggtac aatgtgccag ccagcttttt
      661 tgattgccgt tgatcatatc ccgcaaaaaa gcctcaagtt caggtttgtc cagacccgct
      721 tttttcatcc tccggagcgc ctcgttaatt gccgtttttg tcagcttttt agaggtcagt
      781 aattggttga acatatttcc cgtcttaccg tcgccaatat acgaccaacg cccccacata
      841 cgcagtttcc cctggatcca gacactttcc agcgttttca ggcgtaaatg ctcaccgctt
      901 ttgcctgtaa tttccgggta tatcatattt atgctcactc actttcaatt ttgtaaatct
      961 tcacgcccag ccgcccacca gaaacgagct ggccgcgcac aatattgatt tcatcaaact
     1021 gctcgtcgtc tatgagaagt ccggcatgcg tcagcgcatc cagtggtgct ttcaggatat
     1081 tgtccaggtc acgacggcgc ttatccggtg gctctgcaat aatctttatc gccagccttc
     1141 cggacaggtt taatttcagc cgctgctggc gaacaataag tgccacatcc cggcgataac
     1201 gctcaccggc ttttgataca aaatatgtgc tgccacgacg tcgccagtag gtgttcaccg
     1261 tcggcgggta a
//
LOCUS       DAZFYC010000358         1251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFYC010000358 DAZFYC010000000
VERSION     DAZFYC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.365"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..1163
                     /locus_tag="ACHKW5_005564"
     CDS             183..1163
                     /locus_tag="ACHKW5_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO7514690.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          306 a          309 c          371 g          265 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca
      481 agactgatac cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag
      541 cctgggaagc gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg
      601 cgctgacaga tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga
      661 gaccgagcat tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc
      721 agataaaaga cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa
      781 gcatcccggg tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg
      841 accgcttcgc ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg
      901 aatcaggtag cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc
      961 gcagggcgtt gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt
     1021 tccgcgaccg tctggcggct aatggcaaga aaggaaaggt gattctcggc gcgatgatgc
     1081 gcaagctggc acaggtggcg tatggagtgc tgaagtcagg cgtgccgttc gatgcgtcac
     1141 ggcataatcc ggtagcggcg taaaaatcgc ggaagggatg aaaaaaacag cgcctgacgg
     1201 cgctgtgtct ggcatgcctg caatccggga aaccggacca ggaaaaaact t
//
LOCUS       DAZFYC010000359         1240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFYC010000359 DAZFYC010000000
VERSION     DAZFYC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.366"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1090
                     /locus_tag="ACHKW5_005565"
     CDS             <1..1090
                     /locus_tag="ACHKW5_005565"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7514691.1"
                     /translation="SQGNGIAMLHMTKEKMEQQIIALPPINEQQQIVRKIRELTVLCD
                     QLEQQSLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDTLK
                     QTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPF
                     ELPEGWEWCRIDDLTFVSGGIQKQPKRRPVKNHFPYLRVANVQRGDINIDKLERFEVE
                     PHELAFWSLEKNDILIVEGNGSADEIGRCAIWHAPIEKCVYQNHLIRVRGIIEGYQEF
                     IALYLNSPSGIKEMQRLAVTTSGLYNLSVGKIRGITIPLPPLNQQNLILSRIREYILA
                     CENLKTSTQSAQQTQLHLADALTDAAIN"
BASE COUNT          416 a          253 c          244 g          327 t
ORIGIN      
        1 atcgcaaggt aacggtattg caatgctcca tatgactaaa gaaaaaatgg aacaacagat
       61 aatcgcttta ccgcctataa atgaacaaca acaaattgtc agaaaaatca gagaacttac
      121 agttctctgt gaccaactgg aacagcaatc cctgaccagt ctggatgcac atcagcaact
      181 ggttgaaacc ctgttgggaa cgcttacaga cagccaaaac gccgaggaac tggctgaaaa
      241 ctgggcgcgt attagcgagc atttcgacac actttttacc accgaagcca gcgtggatac
      301 gttaaaacag accattctgc aactggccgt aatgggtaaa cttgtgccgc aggatccgaa
      361 tgacgaaccc gcctctgaac tgctcaaacg aattgcgcag gaaaaagctc aactggtgaa
      421 agaagggaaa ataaaaaaac aaaaaccgtt gcccccgatt agcgatgagg aaaaaccgtt
      481 tgaacttccg gaagggtggg agtggtgtcg tattgatgac ttaacgtttg tttctggagg
      541 aatacaaaaa caaccgaaaa gacgacctgt taaaaaccat ttcccttatt tgcgggttgc
      601 taatgtccaa agaggtgata ttaatattga taaacttgaa agattcgaag tagaacctca
      661 tgagttagct ttttggtcgt tagaaaaaaa cgatattcta attgttgaag gtaatggtag
      721 tgctgatgaa attggtcgtt gtgctatttg gcacgctcca atcgaaaaat gtgtttatca
      781 aaatcacctg ataagagttc gcgggataat tgaaggttat caagagttta ttgcattata
      841 tcttaattcg ccatctggta taaaagaaat gcaacggctc gctgttacca ctagcggtct
      901 atataatctc agtgtaggta aaatcagagg aataacaatt ccattaccac cccttaatca
      961 acagaacttg attttatcaa gaattagaga atatatttta gcctgtgaaa acctaaaaac
     1021 atctacccaa tccgcccagc aaacccaact gcaccttgca gacgcactga ctgacgcggc
     1081 gataaactaa ttttctcgcc ttcaaaaaac caataaagcg gctaaatagc cgctttattc
     1141 acatcagcaa aatttatatt tcacgctaat tttcatctta ttttcattaa tttttagctg
     1201 gactttcctt gtatttactg atgatatatc caggtattta
//
LOCUS       DAZFYC010000360         1237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFYC010000360 DAZFYC010000000
VERSION     DAZFYC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.367"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..577
                     /gene="nleB"
                     /locus_tag="ACHKW5_005566"
     CDS             <1..577
                     /gene="nleB"
                     /locus_tag="ACHKW5_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO7514692.1"
                     /translation="IHKQESYPASERDNYLLGLLREELKNIPEGKDSLIESYAEKREH
                     TWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGIA
                     VHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNYS
                     SLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            626..>1237
                     /gene="nleE"
                     /locus_tag="ACHKW5_005567"
     CDS             626..>1237
                     /gene="nleE"
                     /locus_tag="ACHKW5_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO7514693.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSY"
BASE COUNT          420 a          187 c          255 g          375 t
ORIGIN      
        1 tatccataaa caagaatcat accctgccag tgagagggat aattatctgt taggcttatt
       61 aagagaagag ttaaaaaata tcccagaagg taaggactct ttgattgagt catatgcaga
      121 aaaaagagaa catacttggt ttgatttttt caggaatttg gccatgttga aggctggaac
      181 tttgtttaca gagacgggaa aaactggatg ccataacata tcgccctgta gcggatgtat
      241 atatcttgat gccgacatga ttattaccga taaattagga gtcctgtatg ctcctgatgg
      301 tatcgctgtg catgtagatt gtaatgatga gataaaaagt cttgaaaatg gtgcgatagt
      361 tgtcaatcgt agtaatcatc cagcattact tgcaggcctc gatattatga agagtaaagt
      421 tgacgctcat ccatattatg atggtctagg aaagggtatc aagcggcatt ttaactattc
      481 atcgttacac gattataatg ctttttgtga ttttattgaa tttaagcatg aaaatattat
      541 accgaatacc agtatgtata ccagcagttc atggtaagtc ttccggttgt ggttgaaaaa
      601 tatcgttctt taggtataga ttcagatgat taatcctgtt actaatactc agggcgtgtc
      661 ccctataaat actaaatatg ctgaacatgt ggtgaaaaat atttacccgg aaattaaaca
      721 tgattacttt aatgaatcac ccaatatata tgataagaag tatatatccg gtataaccag
      781 aggagtagct gaactaaaac aggaagaatt tgttaacgag aaagccagac ggttttctta
      841 tatgaagact atgtattctg tatgtccaga agcgtttgaa cctatttcca gaaatgaagc
      901 cagtacaccg gaaggaagct ggctaacagt tatatccgga aaacgcccaa tggggcagtt
      961 ttctgtagat agtttataca atcctgattt acatgcatta tgtgagcttc cggacatttg
     1021 ttgtaagatc ttccctaaag aaaataatga ttttttatac atagttgttg tgtacagaaa
     1081 tgacagccct ctaggagaac aacgggcaaa tagatttata gaattatata atataaaaag
     1141 agatatcatg caggaattaa attatgagtt accagagtta aaggcagtaa aatctgaaat
     1201 gattatcgca cgtgaaatgg gagaaatctt tagctac
//
LOCUS       DAZFYC010000361         1223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFYC010000361 DAZFYC010000000
VERSION     DAZFYC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.368"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..605)
                     /locus_tag="ACHKW5_005568"
     CDS             complement(<1..605)
                     /locus_tag="ACHKW5_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7514694.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTT"
     gene            complement(602..1078)
                     /locus_tag="ACHKW5_005569"
     CDS             complement(602..1078)
                     /locus_tag="ACHKW5_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO7514695.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT          304 a          339 c          284 g          296 t
ORIGIN      
        1 aaggtggtgg tacgctttga ggccagcgtg aaggcatccc cttccccgtc cacgttttca
       61 gggaaacggt cataatccgt cagcgccaca cgacggtaat ccgaagagga aaagacggtg
      121 atcgacggcc agccaatctt caggaaggag ccgtcaagaa acattttatc gtggacgttg
      181 ttgtcattac gggaaggact gaggcgcttg ctgacctccg gactgtggcg aaacgtcctg
      241 gaaagacgcg ttctggaatg ctcacgcgcc ttcgtctcag tcatctgcac caccagcata
      301 tccgccggat cacagatgat gccgtacaca atccagccat caatcagccc ttcggttttc
      361 ccggttcgcg caggtcccac aaacaccacc gcgtcatatt cacgggctga taatgtatta
      421 atggggtcaa tcatataggg cgtcagcgat gactcccacg gaccggaagt attggctccc
      481 cgtggaaccc gcatataacg cctgatggct tccgctactg gtaaccggct gggtgggcga
      541 aacagcgagg ccacttcgcg ccagatatcg gatgcgcggc tatggctctc gttcacctga
      601 ttcacatatc ggcctcatca caacagtcaa tgactgcctt ttccagtgtg tcgcggatct
      661 catcaaccac aatctgtact tcattcagtt gtgatgcggt ccaccccctg tccctctcca
      721 gccggtcagg ccaggtttcc agtacctgaa ctatcgcttt caccacgaca gaaaaggacc
      781 gcctgacatc actgactggc acaagctgac cagtttcctg ctcaaatttc agtcggtcac
      841 gttctgactg gtaccatgct ttacgcgcat gaggatccat ttcctcgtta tctacaggca
      901 gaggagcttt catcagctcg gcaaggatat ctgtcagtcg gtacagtttg agattgctct
      961 catgaccacc ggctgggctt atgtttttta cccgagccgc aacagtctgt cgatgagcac
     1021 cggataatgc ggccagttgg gaaatattca gatgcagatt ttttaattca cgatccataa
     1081 ctccccctga aaattatgta aacacacacc agtgatgaac aaaaaacaac cagattcgac
     1141 actaaaaatt tttatttttc tatatatcaa caacttacac tggtggtgat ggtgccataa
     1201 aaatcaaaaa atgcgccttt ttc
//
LOCUS       DAZFYC010000362         1219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFYC010000362 DAZFYC010000000
VERSION     DAZFYC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.369"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..406)
                     /locus_tag="ACHKW5_005570"
     CDS             complement(5..406)
                     /locus_tag="ACHKW5_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HFO7514696.1"
                     /translation="MMERFKLGDTATYTQTITDADIKSFAGISGDNNPVHMSDEYAEG
                     SRFKKRIAHGLISASFFSALFGTKLPGPGCVYVNQSLKFLRPVYINDTVTARVVLTDI
                     DVVKRRLFFDTICEVNRKKVITGKAEIYLPE"
     gene            complement(403..969)
                     /locus_tag="ACHKW5_005571"
     CDS             complement(403..969)
                     /locus_tag="ACHKW5_005571"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFO7514697.1"
                     /translation="MNIIGKTVKLRAVEIDDLELLNKWANDPEIWYMLGGWHFPYSKN
                     NTEKWIKNIDNNDSKNQIFAIETEEHGLIGTANLVNIDWKNKNAFHGIMLGNVETRGK
                     GYAQDVVMSLMRYAFDELGLNRLDGDMIEYNKLSINFYIKRCGWKIEGIKKEWFFRKG
                     QYFDKVVVGITKKEYLEHIEKSKYWETK"
     gene            complement(969..>1219)
                     /locus_tag="ACHKW5_005572"
     CDS             complement(969..>1219)
                     /locus_tag="ACHKW5_005572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514698.1"
                     /translation="IKIPEIVNGPLCYPLLSKNNKLKDILIKNEIYVPTYWKDVLNRV
                     DINSPEFEFVSNLIPLPCDQRYSSIQMKKIINIVLEEN"
BASE COUNT          370 a          250 c          149 g          450 t
ORIGIN      
        1 cgctttattc tggcaagtaa atttcagcct tccccgtgat aacttttttt cggttaactt
       61 cacaaatcgt atcaaaaaag agtcttcttt ttacaacatc aatgtctgtt aagactacac
      121 gcgctgtaac cgtatcatta atataaacag gtctaagaaa tttcaagctt tgatttacat
      181 atacacatcc aggcccaggt aattttgtac caaatagagc tgaaaaaaaa ctagcagata
      241 tcagaccatg agctatacgc tttttaaacc ttgaaccttc tgcatattcg tcactcatat
      301 gtactggatt attatcccca gatatacctg cgaagctttt aatatctgca tcagttatag
      361 tttgagtata agttgctgtg tcccccaatt taaaacgttc catcattttg tctcccaata
      421 cttgcttttc tcaatatgtt ctaaatattc ttttttggtt attccaacaa ctactttatc
      481 aaaatattgc ccctttctaa agaaccactc tttcttaatt ccctcgattt tccaaccaca
      541 tcttttgatg taaaaattaa ttgataattt attatattca atcatatcac catcaagtcg
      601 atttaatcct aattcatcaa aggcatatct catcagactc attacaacgt cttgcgcgta
      661 ccctttacct cgtgtttcaa catttcccaa cattatccca tgaaatgcat ttttattttt
      721 ccaatcaata tttactaagt tagcagtgcc aatcaatcca tgctcttcag tttcaatagc
      781 gaatatttga tttttcgaat cattattatc aatatttttt atccattttt cagtattatt
      841 tttggaatag gggaaatgcc aaccaccaag catataccaa atttccggat cgttagccca
      901 tttatttaac aattctaaat catcaatttc cacagcacgc aacttaactg tttttcctat
      961 gatattcatt agttttcctc aagtacaata ttgattattt tcttcatctg gatagatgag
     1021 taacgttgat cacatggcag aggtattaag tttgagacaa attcaaattc tggcgagttt
     1081 atatctactc gattcaatac atctttccaa tatgtaggca cataaatttc attcttgatt
     1141 aaaatatctt tcagtttgtt gttttttgaa agcaatggat agcataatgg accattgact
     1201 atctccggaa tttttattc
//
LOCUS       DAZFYC010000363         1219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFYC010000363 DAZFYC010000000
VERSION     DAZFYC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.370"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..67
                     /locus_tag="ACHKW5_005573"
     CDS             <1..67
                     /locus_tag="ACHKW5_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7514699.1"
                     /translation="AIKAKYQQKLKDLRNSRSEAA"
     gene            64..264
                     /locus_tag="ACHKW5_005574"
     CDS             64..264
                     /locus_tag="ACHKW5_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO7514700.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYIGDKEGKR
                     HAVVNGVLMVHRGWDKGKDSDA"
     gene            257..682
                     /locus_tag="ACHKW5_005575"
     CDS             257..682
                     /locus_tag="ACHKW5_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7514701.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVSWSPIAAGFKGLIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMSLGRKHGRSDCWAGRVLQKA
                     EGVIEGMLIMQGVKLEMDRYVEREPSGSQASQFAGRAGN"
     gene            882..1037
                     /locus_tag="ACHKW5_005576"
     CDS             882..1037
                     /locus_tag="ACHKW5_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7514702.1"
                     /translation="MKQQKAMLIALIVICITVVVIALVTRKDFCEVRIRTGQTEVAVF
                     VDYESEK"
BASE COUNT          333 a          257 c          344 g          285 t
ORIGIN      
        1 ggccatcaag gcgaagtatc agcagaaact taaagacctg cgaaacagca gaagtgaggc
       61 cgcatgacgt tcacagtaaa aaccattcct gacatgctcg ttgaggcata tgaaaatcag
      121 accgaggtag ccagaatact gaactgtagt cgcaacacgg tcagaaaata tattggcgat
      181 aaagaaggca aacggcacgc cgtcgtcaac ggtgtcctta tggttcaccg tggatgggat
      241 aaggggaaag actctgatgc gtgatattca gcaggtgctt gaacgctggg gcgcatgggt
      301 ggccaataat catgaggatg tgagctggtc gcccattgcc gccggattta aggggctgat
      361 cccgtcaaag gttaaatcac ggcctcagtg taccgatgac gatgcgctga ttatcagcaa
      421 ttgcatggcg caactgaacg tcaataacag cgatttgcat gattttctgt atgattatta
      481 tgtgttcggg atgacgctta tgtcactggg ccgtaagcat gggcgctctg attgctgggc
      541 cgggcgggta ctgcaaaaag cagaaggtgt tattgagggg atgttgatca tgcagggagt
      601 aaagctggaa atggacaggt acgttgagcg tgaaccatca ggatcacagg ccagtcagtt
      661 tgccggacgt gcgggaaatt gaaagcgcga gtttttactg tagaatagct gcgggtgctt
      721 gaggctgtct gcctcgggca tgcagccgta aggcagacag agaaaagccc cagttaacat
      781 tacgcgtctt gcaggacgct taacattaat ctgaggccaa atctatgact tacaaactta
      841 ggttagcctc ttacgtgccg aaaggcaagg agaagtaggc tatgaagcag caaaaggcga
      901 tgttaatcgc cctgatcgtc atctgtatta ccgtcgtagt gatagcactg gtaacgagga
      961 aagacttctg cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcgtagact
     1021 acgaatctga gaagtaagag accaggcagg ggagtaatct cccgccacct ctgatgtgtc
     1081 tggcatcctc aacgcacccg tactttattt gtcatatgct ccgcaaaatg aaggttgtcg
     1141 ttgcaaccaa tgcggtaatt ttttgtgtgc gagtgctcaa aaaacgttga ttttcataaa
     1201 aggatgattt tatgctgcc
//
LOCUS       DAZFYC010000364         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFYC010000364 DAZFYC010000000
VERSION     DAZFYC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.371"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..>1212
                     /locus_tag="ACHKW5_005577"
     CDS             join(52..310,310..>1212)
                     /locus_tag="ACHKW5_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7514703.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKT"
BASE COUNT          328 a          313 c          327 g          244 t
ORIGIN      
        1 gccacctgat gaaaggtgat attctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gaaccttcac ccctgagttc aggctggaat gtgcacagct gattgttgat
      121 aagggctact catatcgaca ggccagtgaa gcgatgaatg tcggttcaac cacgcttgag
      181 agctgggtac gccagctcag gcgagagcgc cagggtatta cgccctctgc cacacccatt
      241 actccagacc agcaacgtat ccgcgagctg gaaaagcaag ttcgccgtct ggaggaacaa
      301 aatacgatat taaaaaaggc taccgcgctc ttaatgtccg actcgctgaa cggttcacga
      361 tagccgccag actaagtgac agccacacgg ttgtcagcct gtgttctgct ctggaaatac
      421 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcacgagtca
      481 ggttgtgcag cgaaatacgc cgggcgtgga accaaagtcg gggctctgcg ggcgcgcgca
      541 ctctggctga aatgctgact caaaacggcg tcccgatgag ccgttaccgt gccggacgtc
      601 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtat aaaaatgccc
      661 gtcaggagca taccagcctg ccgaatctgc ttgagcgtca gttcgcagta ccggagccag
      721 accgggtatg gtgtggagat attacgtatc tctgggcagg aaatcgctgg tgctatctgg
      781 cggttgttat ggatcttttt gcccgcaggg ttatcggctg gagtctgtca gcgcatgccg
      841 ataccgcact gataagcagt gccctgcgga tggcctatga gacgcgtggc caaccccggg
      901 atgtcgtgtt ccatagcgac cagggaagcc agtatacagg ccttaaatat caacaacttc
      961 tctggcgttg cagaataaat caaagcgtca gtcggcgggg aaactgctgg gataacagcc
     1021 ccatggaacg cttcttccgc agtctgaaaa cagaatgggt gccaacgaat ggttacgcag
     1081 gcaaggacga ggcccggcag caaattaatg attacatatt gaactactac aacagcgtca
     1141 gacctcacca ttataacggt gggctgacgc cggaagagtc agagaacaga taccattttt
     1201 actgtaaaac cg
//
LOCUS       DAZFYC010000365         1199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFYC010000365 DAZFYC010000000
VERSION     DAZFYC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.372"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1199
                     /locus_tag="ACHKW5_005578"
     CDS             <1..>1199
                     /locus_tag="ACHKW5_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514704.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPE
                     VSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRY
                     AVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYKGQPVTRLAVMLN
                     LLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQA
                     VAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQEVCGHGFRAMAC
                     SALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREKF
                     ISPFEYA"
BASE COUNT          325 a          290 c          287 g          297 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggtt catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa ggagaagact ctggcacttg
      121 gtgtgtatcc agaagtttct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcaga ggggattgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc ttttgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaggta ctcaaaagcc tcgaaacaca cgttttcccc tttatcggca
      361 accgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aatatctgct gtaatgcgtt
      481 atgctgtaca gtctggcatc atcagatata acccggctct ggatatggct ggtgcattga
      541 ccacggtaaa acgccagcat cgccctgctc tggatctttc acgcctgcct gaacttctgt
      601 cgcgtattga cggttataaa ggccagcctg tcacccgact ggcagttatg ctgaatttac
      661 tggtttttat tcgttccagt gaactcagat acgcccgctg gtctgaaatt gatattgaca
      721 atgccatgtg gactattcca gccgaacgcg aacctctgcc aggcgtaaaa ttctcacacc
      781 ggggctccaa gatgcgaaca ccacatcttg tgccactcag caaacaggct gtagccatac
      841 tgacagaact tcagacatgg gcaggtgaaa atggtctgat atttacgggt gcacatgacc
      901 cgcgtaaacc aatcagtgaa aatactgtaa ataaagccct gagggtgatg gggtatgaca
      961 caacccagga agtctgtggc catggattcc gggcgatggc gtgcagtgca ttgattgaat
     1021 caggtttgtg gtcccgcgat gctgtggaac gtcagatgag ccatcaggaa cgtaacggtg
     1081 tacgtgctgc ttacattcat aaagcagaac atctggaaga acgccgcttg atgctacaat
     1141 ggtgggccga ttttctggat gcaaacagag aaaaatttat cagtccattt gaatatgca
//
LOCUS       DAZFYC010000366         1197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFYC010000366 DAZFYC010000000
VERSION     DAZFYC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.373"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..978
                     /locus_tag="ACHKW5_005579"
     CDS             1..978
                     /locus_tag="ACHKW5_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514705.1"
                     /translation="MSAFHNGRILVEPEPKSMRALPSGVVPAVHQPLAEDKSLLPFFS
                     DERVSRAAGGAGALSDWLLRHVKSCQWLHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPPQEKTVVS
                     IAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTK
                     SHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            991..>1197
                     /locus_tag="ACHKW5_005580"
     CDS             991..>1197
                     /locus_tag="ACHKW5_005580"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7514706.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRD"
BASE COUNT          282 a          314 c          336 g          265 t
ORIGIN      
        1 atgtcagcat tccataacgg cagaatactg gtggagccgg aaccaaaaag catgcgagct
       61 ctgccgtccg gggttgtacc tgccgttcac cagccgctgg cggaagataa atcactactg
      121 ccatttttca gcgatgagcg ggtgagccgt gctgcgggtg gcgctggtgc actgtctgac
      181 tggttattac gtcacgtgaa atcctgccag tggctacacg gtgattatca tcacagcgaa
      241 accgtcattc accgttacgg taccggcgca atggtgttgt gctggcactg cgacaaccag
      301 ctgcgtgacc agacatccga atcactcgag caacttgctc atcaaaacct gtcagcatgg
      361 atgattgacg tcatcggtca cgcaataagc ggtacgcagg agcgtgaatt atctctggct
      421 gaattatcct ggtgggcggt ccgcaatcag gtggcggacg cgctaccgga agcggtatta
      481 cgtcgttcgc tggggttgcg tgcggaaaaa atccgctcca tgtaccgtga aagcgacatc
      541 gtaccgggag agcagaccgc caccagcata ctgaaacagc gcacaaaaaa tcttgcgccg
      601 ctgcctcacg cccaccagca acagaaccca ccacaggaaa agacggtggt cagcattgcc
      661 gttgatcctg agtctccgga atctttcatg aaacgaccta aacgtcgccg ctgggttaac
      721 gagaaataca ctcgctgggt gaagacacag ccgtgtgcgt gttgtggtaa gccagccgac
      781 gatccccatc acctgattgg tcatggtcag ggcggaatgg ggacaaaatc tcacgatatt
      841 ttcacgctac cgctgtgtcg ggagcatcac aacgagcttc atgcggatcc gctggcgttc
      901 gaagaaaagc atggttctca ggttgattta atttttcgtt ttcttgatca cgcctttgca
      961 actggcgtgc ttgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg
     1021 ccgacggtga acacttactg gcgacgccgt ggcagcacat attttatctc ggaggaggga
     1081 aagcgttatc gccgggctgt ggcgcttatt gttcgccagc agcggctgaa attaagcctg
     1141 tccggaaggc tggcgataaa gattattgcc gagccaccgg ataagcgccg ccgtgac
//
LOCUS       DAZFYC010000367         1194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFYC010000367 DAZFYC010000000
VERSION     DAZFYC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.374"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..145
                     /locus_tag="ACHKW5_005581"
     CDS             <1..145
                     /locus_tag="ACHKW5_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO7514707.1"
                     /translation="IDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQD
                     RVQ"
     gene            189..1103
                     /locus_tag="ACHKW5_005582"
     CDS             189..1103
                     /locus_tag="ACHKW5_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514708.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            1113..>1194
                     /locus_tag="ACHKW5_005583"
     CDS             1113..>1194
                     /locus_tag="ACHKW5_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514709.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNAS"
BASE COUNT          371 a          214 c          242 g          367 t
ORIGIN      
        1 tattgatttt gtcacaccgc atgctgtcct ggaaaaagaa ctggaaccac aggagagaat
       61 tgcctttatt tatgaagata tttcctggga gcatacgtta gccggtacga atgcaatgag
      121 taaatggcag gatcgtgtac agtaacttac tgtaataaca taataaatta ctattgcgga
      181 gagataaaat ggctggttta aggaataaca acaacacaca gaatgcacag tgggctgatt
      241 atgtgggtga cattcttcgg ggggctcagc cgataaacca gctagtacct cagcaccctt
      301 atcttaacga cgtcccactc attgatgagt taaggcatca gaacactcat catgttattc
      361 tcttgacact tgatgttgca aaaaaaatac tcagcccaat aacatcattt gattacattc
      421 attttatcac tacacatcct tcaggtataa aagatacact cgcctggtta gttaacgcgg
      481 gtaaattaat gactgagttt gatgacaatg ggaaaattat atttaacctt aatgccttaa
      541 agtatacaaa agcaagttac tttgaaatac ttggagagaa atatataaaa atcacaacat
      601 ccagcccctg gcttttagaa aagctcggta aatatatttt ctcttcacga gcgcctcaag
      661 ttctggaact cgctattggc tggcgaggag ctttatctga atcaataaaa ggagttaagt
      721 tctgtatctg gttttcagta gcctggagaa ccatagaatt tattatgagt tcagagcgag
      781 atctggttaa ttttcttggc gatttttcaa tggatgtggc caaagcagtt atagccggtg
      841 gtgttgcaac cgctattgga agcctggctt cttttgcctg tgttagcttt ggctttccag
      901 taattcttgt cggaggagca attttactga cagggatagt gtgtacagtt gttttaaatg
      961 aaatcgatgc tcaatgccat ttatcagaaa aattaaaata tgcaattaga gatggactaa
     1021 aacggcaaca ggaacttgat aaatggaaaa gggaaaacat gactccattt atgtatgttc
     1081 ttaacactcc acccgtgata tgaggattaa ccatgcgttt acatgttaaa ttaaaagagt
     1141 ttctttcgat gttttttatg gccatattat ttttccctgc attcaatgct tctt
//
LOCUS       DAZFYC010000368         1189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFYC010000368 DAZFYC010000000
VERSION     DAZFYC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.375"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(152..849)
                     /locus_tag="ACHKW5_005584"
     CDS             complement(join(152..600,600..849))
                     /locus_tag="ACHKW5_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7514710.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVLGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARPGRKSLSFSKSVELH
                     DKDIGHYLNIKHYQ"
     gene            complement(1110..>1189)
                     /locus_tag="ACHKW5_005585"
     CDS             complement(1110..>1189)
                     /locus_tag="ACHKW5_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514711.1"
                     /translation="QIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT          309 a          312 c          271 g          297 t
ORIGIN      
        1 aagattacaa gatattttta cataaaataa tattacgcgc taccacctca gaaaccacgt
       61 aagtacatat gctattacat agataaccgc catgatcagg gaagccccat acaagcacag
      121 tgtagagagg cctttaggta atgactccaa cttactgata gtgttttatg ttcagataat
      181 gcccgatgtc cttgtcatgc agctccaccg attttgagaa cgacagtgac ttccgtcccg
      241 gccttgccag gtgttgtctc agattcagat tatgtcgctc aatgcgctga gtgtaacgct
      301 tgctgataac gtgcagcttt cccttcaggc gtgattcata cagcggccag ccatccgtca
      361 tccataccac gacctcaaag gccgacagca ggctcagaag acgctccagt gtggccagag
      421 tgcgttcacc gaggacgtgc gccacaaccg tcctccgtat cctgtcatac gcgtaaaaca
      481 gccagcgctg acgtgattta gcaccgacgt agccccactg ttcgtccatt tcagcgcaga
      541 caatcacatc actgcccggt tgtatgcgcg aggttaccga ctgcggcctg agttttttaa
      601 gtgacgtaaa accgtgttga ggccaacgcc cataatgcgt gcactggcgc gacatccgac
      661 gccattcatg gccatatcaa tgattttctg gtgcgtaccg ggctgagagg cggtgtaagt
      721 gaactgtagt tgccatgttt tacggcaagg agagcagaga tagcgctgat gtccggcagt
      781 gcttttgccg ttacgcacca cgccttcagt agcggagcag gaaggacatc tgatggaaat
      841 ggaagccacg caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat
      901 taccgtgatt gttttgtcgg aactgatacg ctggcgctaa taggtagtag ggttcttatg
      961 ctaaacatac aacctctttc ttaattctcg aacctgttta ggattctgtg taaattcaaa
     1021 ataaacctgt ctgaacattc ctaacaaaca tccaccggac atgacaacaa aaaaccggag
     1081 ccggactccg gtttttgtga agctgtcggc tatttcattc cgccaatatt ttcccacctc
     1141 ccgtcagcac gcaggatttg cagcggtctt accacgcact gtatctgct
//
LOCUS       DAZFYC010000369         1187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFYC010000369 DAZFYC010000000
VERSION     DAZFYC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.376"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1187
                     /locus_tag="ACHKW5_005586"
     CDS             <1..>1187
                     /locus_tag="ACHKW5_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7514712.1"
                     /translation="ASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLT
                     TKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLF
                     TDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNSTYSGSQQNTGASDTTAQPE
                     PVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQ
                     NQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAV
                     MGDATYQETFDEEYQVEVQEDDPEKMEGAEHPHKENTGGNQHHDSDNETGETADHSIK
                     VNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLE
                     TAGG"
BASE COUNT          385 a          291 c          295 g          216 t
ORIGIN      
        1 gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat ttttggcctt
       61 gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa atacattact
      121 gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag cgcaactgat
      181 actctctcgt cgccagtacc agaaactgat gcagtggaaa cggcaattcc ggacaacgaa
      241 aaaaccgaat gcaaagtgga agtcgaacca tctgtagagc gtgaggggcc gttctacttc
      301 ctcttcaccg acaaggatgg cgaaaaatac ggtcgcgcaa acaaactttc tggtctggat
      361 aaggcactgg ctgccggggc tactgaaatc acgaaagaag aatatttcgc ccgcaaaaac
      421 agtacatact caggttcaca acaaaatact ggtgcatctg acacgaccgc acaaccagag
      481 ccggtaaaag ttaccgctga cgaagtaaac aaaattatgc aggcagccaa tatcagccag
      541 cctgacgccg ataagttgct tgctgcctct cgcggagaat ttgttgcagg gattagcgac
      601 ccgaatgatc cgaaatgggt taaggggatc cagacccgcg attctgtaaa ccagaaccag
      661 catgaatcgg aacggaacta ccaaaaagcg gaacaaaaca gcccaaatgc gttacaaaac
      721 gagccagaaa cgaaacagcc tgaaccagtg gcgcaacagg aagtggaaaa agtctgcacc
      781 gcctgcggtc agaccggcgg cggcaactgc cctgattgtg gcgcagtgat gggcgacgca
      841 acataccagg aaacattcga tgaagagtat caggttgaag ttcaggaaga tgatccggag
      901 aaaatggaag gcgctgaaca tccacacaag gagaacactg gcggcaatca gcatcacgat
      961 agcgataatg aaactggcga gacggcagat cactcaatta aggtgaacgg tcatcaagaa
     1021 atcacatcca ccagcaggac gtgtgaccat ctaatgatcg accttgaaac catgggaaaa
     1081 aatcctgatg ccccgatcat ctcaataggt gcaatatttt tcgatccgca aaccggagat
     1141 atgggaccgg aatttagtaa gactatcgat ctggaaactg ctggcgg
//
LOCUS       DAZFYC010000370         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFYC010000370 DAZFYC010000000
VERSION     DAZFYC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.377"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            344..475
                     /locus_tag="ACHKW5_005587"
     CDS             344..475
                     /locus_tag="ACHKW5_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514713.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLG"
     gene            698..904
                     /locus_tag="ACHKW5_005588"
     CDS             698..904
                     /locus_tag="ACHKW5_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7514714.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            969..>1177
                     /locus_tag="ACHKW5_005589"
     CDS             969..>1177
                     /locus_tag="ACHKW5_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514715.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAA"
BASE COUNT          289 a          258 c          315 g          315 t
ORIGIN      
        1 tcccggcgag atcaggaaag tgcgctggcg tgctggggta tcgacagata agcagaatat
       61 tttgcttaaa aatgacgttg gccaacgcgg gcagataaca cgaaatcctg cgaactggca
      121 aaacccaagt gaataaaagt aaaaaccccg tttgttggtt gcaaacgggg ttttatgttt
      181 atggcagtaa gctatgggag gctgccttga ttgattttag caaactgatt agggagttgc
      241 gactcatgat tagtcaatta ccaaactgga aatttttgct ggtctggagc atcccttttt
      301 tatgggtagt atcccagtga attgtggcaa ttaaggggta gctatgtcag acaaactcat
      361 aatgctggcg aagatcctct gtgtaatcgt cggcatttca ttttcactaa tgctggttgc
      421 tctttttctt tccatgggct ggatgatgtt gtcttcgtcg gggctgctgg ggtgagcata
      481 aaccgaatgc tttccgcgtt taccgttatt ctgctggtgg tctgtggtgc gcttagtctg
      541 gggctgaatc attaccgcga taacgccatc acctacaaag cgcagcgcga taaaaaagcc
      601 agtgagctgg atctggcaaa cgcaaccatt actgatatgc aggtgcgcca gcgcgatgtt
      661 gctgcgctcg atgcaaaata ctcgagggaa ttagccgatg cgagagctga aaatgaaact
      721 ctgcgtgctg atgttgccgc tggtcgtaag cgcctgcgga tcaacgccac ctgccccggt
      781 accgtgcgtg aagccaccgg cacctccggc gtgggcaatg atgccgccgt cgaactctct
      841 ccggttgctg gacgaaacgt tctcggtatc agagacggaa tcatcagcga ccaggcagca
      901 ttgagaatgc ttcaggaata tatccgcact cagtgtatta actagtattt ttgttattcg
      961 gagaatgcat gaagaaatta ttggtaaccg taaagccttt tcagggaaca attccgttcc
     1021 gtattttgca gcgtggtcgt gttcttgttg aaggttcgtt cagtggtaaa tgtacgcaat
     1081 tacactcccg gacctttcag gtgaatgcca cgaatgaaga gctaaccgtt gagtgtacga
     1141 tgaatgccgc taaatgccgc atggtatccg ctgcatt
//
LOCUS       DAZFYC010000371         1149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFYC010000371 DAZFYC010000000
VERSION     DAZFYC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.378"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1149
                     /gene="nleA"
                     /locus_tag="ACHKW5_005590"
     CDS             <1..>1149
                     /gene="nleA"
                     /locus_tag="ACHKW5_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO7514716.1"
                     /translation="LAHPPHPAPNQIPESELPPGCETGFVVNIPEDIQQHTPECGETT
                     ALLGLIRDKGLLSGLDAYLAPHLEEGSAGKKALDMFGLFNVTQMALEIPSTVPGISGK
                     YGVQMNIVKPDIHPTTGNYFLQLFPLHDEVGINFGALPGPLQKFLSQNPNPWFGVTAQ
                     VVRNHGIELPIVKTDNGWKLVGETPLTPEGPKANYTEEWVIRPGEFDLKYGSTPLQST
                     LGLEFGAHFKWDLDNPNTKYAVLTNALANAAGAAGGYTLSQYTGTDAMLSPHVGSMAG
                     QALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIF
                     PNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNDLPHHNPS"
BASE COUNT          347 a          251 c          263 g          288 t
ORIGIN      
        1 tcttgcacac ccaccacatc ctgcgccaaa tcagataccg gaatcagaat tgccacctgg
       61 atgtgagact ggatttgttg ttaatattcc agaggatata cagcaacaca ctccggaatg
      121 cggtgaaaca acagctctgt tgggtttaat aagagataaa ggtctgctct cagggttaga
      181 cgcatatctt gctcctcacc ttgaagaagg ctcagcagga aaaaaagcat tggatatgtt
      241 tggtttattc aatgttactc agatggcatt agaaataccc agcaccgttc ctggtatctc
      301 tggtaaatat ggtgttcaga tgaacattgt aaaaccagat attcacccca caaccgggaa
      361 ttatttttta cagttattcc ccctacatga tgaggtggga ataaacttcg gtgcgctccc
      421 aggaccactc cagaagtttc tctctcaaaa tcccaatcca tggtttggag taacagctca
      481 agttgttcgt aaccacggca tagaactacc tatagtcaaa acagataatg gatggaaact
      541 agttggagaa acaccactta cacctgaagg gcctaaagca aattatacgg aagaatgggt
      601 catcagacct ggtgagtttg acttaaagta tggcagcact ccgttgcaga gcactttagg
      661 tttagaattt ggagctcatt ttaaatggga tttagataat cctaatacca aatatgccgt
      721 tcttaccaat gcacttgcga atgcagctgg tgctgcaggg ggatatactc tatctcaata
      781 tacaggaaca gacgcaatgc tgagccccca tgttggctcg atggccggac aggctttggg
      841 gcatgctgta caatgctaca ctccaggatt aaagccagac actattttat ggtgggcagg
      901 tacgactctg gggctggcag atttaaacaa agccgagaca ggtgaaggca gattcactga
      961 ctatcctcgt atatggtggc acgcaagaga aggtgccatc ttccctaata aagaagatat
     1021 tgcccgtgca acaggggctg atatacgagc tatggaagaa ggtatccctg ttggacagcg
     1081 gcatccaaat ccagaggatg tggtaatcga tatcgaaagc aatgacttac cacatcataa
     1141 tccatcaaa
//
LOCUS       DAZFYC010000372         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFYC010000372 DAZFYC010000000
VERSION     DAZFYC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.379"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..467)
                     /locus_tag="ACHKW5_005591"
     CDS             complement(<1..467)
                     /locus_tag="ACHKW5_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO7514717.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYD"
BASE COUNT          343 a          268 c          250 g          286 t
ORIGIN      
        1 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
       61 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
      121 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
      181 tgcaccttca tgctgtacag catctggttt ttgtcgttct cccccagttc aaaccaggtc
      241 accacacctt tcacccggcg ctgtacgtca tcgccctgcc ataccgtcag gtaggccatt
      301 ttgtccagca cctgcgcaaa ttcaagggaa agaaactgct gactgaccag agaaaggtcg
      361 agggaaaaaa gtgaagagag tgactgggtg agatgaaagg aaaccaccgc aaacgcgtcc
      421 ggtggcaggc cgtccacttc aagtgtgaaa cgtaatccgg ttgacatagc aacctccgtg
      481 aaaatgaata ataatccgca catattgcat gtacaacatg tcgggcggat gagataagcg
      541 agtcgacatc tggcgttacc ggggaatatc agaaaaatgt catggttatg tgatgattta
      601 gtctgggatt ttacttcaga gaaacgggtg tctgtttagt caacaaaata atcacaaacg
      661 caacggatgg gttctcgacg ttattcacga caggctcgta cattacagcg agtaataaaa
      721 acctgatatg caaagtcaaa ccaatattca taatgtcttt ttattcacaa caataaataa
      781 ttcaataaca atcattgaaa tgaatgatga tttgtataag gaatatacgc taccgcccca
      841 ccctgataac atgatctgca cattgccatc ttaacgtcgt taaacactgg cttaatagtg
      901 gatatttcaa attaggaatt tattaaattc acctgatgaa tatcataagc aaatagcata
      961 gaaaataatg acaattcccc cgaggggacc atttccaaag cactgcacca ccacctggtg
     1021 cccggatgat cttgcttcag tggtaagagt acctgattca ttgttcccaa aatgatgttt
     1081 tccccggccc cgccaataat gtgcagctct cacgtgataa tctattgatt aggttatatt
     1141 aagaatc
//
LOCUS       DAZFYC010000373         1145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFYC010000373 DAZFYC010000000
VERSION     DAZFYC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.380"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHKW5_005592"
     CDS             <1..159
                     /locus_tag="ACHKW5_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514718.1"
                     /translation="GTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDH
                     AFATGVLG"
     gene            172..546
                     /locus_tag="ACHKW5_005593"
     CDS             172..546
                     /locus_tag="ACHKW5_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7514719.1"
                     /translation="MRIEFVLLYLPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRAQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            543..>1145
                     /locus_tag="ACHKW5_005594"
     CDS             543..>1145
                     /locus_tag="ACHKW5_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7514720.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVK"
BASE COUNT          295 a          242 c          336 g          272 t
ORIGIN      
        1 ggaacaaaat cccacgatat tttcacgcta ccgctgtgtc gggagcatca caacgagctt
       61 catgcggatc cgctggcgtt cgaagaaaag catggttccc aggttgattt aatttttcgt
      121 tttcttgatc acgcctttgc aaccggcgtg ctcgggtaaa agaggttact gatgcgtata
      181 gagtttgttt tgctttacct gccgacggtg aacacctact ggcgacgtcg tggcagcaca
      241 tattttgtat caaaagccgg tgagcgttat cgccgggctg tggcgcttat tgttcgccag
      301 cagcggctga aattaagcct gtccggaagg ctggcgataa agattattgc cgagccaccg
      361 gataagcgcc gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc gctgacgcat
      421 gcggggttgc taatggacga tgagcagttt gatgaaatca atatcgttcg tgctcagcca
      481 gtatctggtg gacgtctggg ggtgaagatt taccccataa tgcttgaagg gcaggtcaaa
      541 aaatgaaact ggaagattta ccgaaatact actccccaaa atcccccggc ctgactgatg
      601 catcggcctc aacgtcgaaa gatacgctga gtatcactga tgtgatggct gcgcagggca
      661 tgacacagaa ttgggctgag atggggtttt ctgcgttcct tgggaaaatg ggcattagta
      721 tgaatgacag agagcgggca acagaattgc tgacagaata tgcactcagt cggtgtgatc
      781 gcgtggcggc gttaagaaaa ctcccggcag aaataaaacc ggcagtgatg cgtattatgg
      841 cttcgtatgc gtttgaagat tatgcccgta gcgcggcgag caaaaaacag tgcccctgtt
      901 gtcacggaaa aaaatttatt gaaagcgagg tttttacaaa caagatccag tatccggatg
      961 gtaagccgcc agtgtgggca aagtgcacaa aaggcgtgta tccgtcttac tgggaggaat
     1021 ggaaaaaagt cagggaggtg gtaaaagttg cctgtccgga gtgtggaggg aagggggagg
     1081 tttccaccgc ctgtaaagat tgtcgtgggc gcggtgttgc cattcatcgt gaagagtcgg
     1141 taaaa
//
LOCUS       DAZFYC010000374         1135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFYC010000374 DAZFYC010000000
VERSION     DAZFYC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.381"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..377)
                     /locus_tag="ACHKW5_005595"
     CDS             complement(<1..377)
                     /locus_tag="ACHKW5_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514721.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLMDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTS"
     gene            511..1038
                     /gene="sodC"
                     /locus_tag="ACHKW5_005596"
     CDS             511..1038
                     /gene="sodC"
                     /locus_tag="ACHKW5_005596"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7514722.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          260 a          331 c          276 g          268 t
ORIGIN      
        1 gaggtggtct ccaccagtga ctgcaccccg aaggtaaccc gcaggcggtc aatgttcgcg
       61 gacgtaatgg tgcgcgtcac cggctttgcc ttcgtcactt ccacgcccag tccggtttca
      121 gctccggagg actcaaagcc ttccggtggt gtctgctcct gctccccggc acgccagacc
      181 gcagtcacac cgtgtatcac gggattaccg tccgtgtcca tcagcggggt tttgttcacc
      241 aggatactct gcagcccctt caccgggcct tctatcggtc cctcaccaat cgcatcaatc
      301 acactcatca tctgcgtgga tttgagatta tccttcgcct ctcgaggcgt gtgcgccctg
      361 ccgccacctt tgcccataat gttcctctca attggtatta ttaatcgcag tgataggata
      421 ttgcacagct attgcgcgat atcatcagaa cgctgtttgt taccctgtaa ccagcaagct
      481 cagtctgtta acggaattaa tgagggtttt atgaaatgta aaatcattgc tgccattgcc
      541 atgctgacag cagcatcatg cggatacgca gcagaacagg aagtcccaat gaaccttgtc
      601 agtgctgacg gaaaagaagt cagcattgga aaaataacca ttcaggagac cccctacggt
      661 ctgctgttca caccagccct tcactctctg tctgaaggca ttcatggttt tcatgtgcac
      721 gaaaaaggaa attgcgcccc ggcactgaaa gacggaaaac cggtcgcagc attatcggct
      781 ggcggtcact ttgacccgaa aaacaccggc aaacatcttg gcccctggtc tccggatgga
      841 cacctgggcg acctccctgc gctgttcgtg acgcatgacg gaaaagcgaa ctacccggtc
      901 ctggccccga gactgaactc attaaaagag attaaagggc gttctctcat gcttcatgct
      961 ggcggtgata accatcatga ccatccggag cccctgggcg gtggtggtgc gagaatggcc
     1021 tgcggcatca ttcaataatc agtcaggtaa ggggcgggcc ccttaccttt attcctcagg
     1081 acgataaatc ctttctccct gaaaagaacg gcacatcctc cctctctgag ttaat
//
LOCUS       DAZFYC010000375         1125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFYC010000375 DAZFYC010000000
VERSION     DAZFYC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.382"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..268
                     /locus_tag="ACHKW5_005597"
                     /pseudo
     CDS             1..268
                     /locus_tag="ACHKW5_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          334 a          258 c          293 g          240 t
ORIGIN      
        1 caaaaggggt aagctgaaat cgtttatgga ttacatcgac atatgcctgg cagagaatgt
       61 ccggaatgac gaggttatct ttgcggagat taaggcaatg gtatacgggc cgacgttcca
      121 tgccgcgtta ctacattcag cccggacgta aaatgcgccc gtcaaaaaga acggttcgct
      181 tcgaaactca gccaggatac cacctctccg ccacacccgg gaagaagttg atatggtggt
      241 tgccggacaa cagcgctcct ggattgactg ctgcatcact caaccacgct gaatatcaaa
      301 ggagagcgtt accggttaaa agagaaacgt aaagctgtgg tgatgaccca aaacgcaacg
      361 cctgtcagtg aggatgaaat ggtgaaaagc ggacagcatc agtaacggaa gtatcttagc
      421 gggtacgaaa atggcaggat cgtgacattg acagaatatc tcctgaccat cctgaatgac
      481 tgcagttgct gtagaaaagt aagctgtctc cacccacggc accacttaac aggcattccg
      541 taacttcccc cagacttact atgacgcatt atcggtgcac tgaaaacggg agagtgcgct
      601 taaaaccgca gaccgcatgc gggtgttact gcatgacagt ctggccgtat tgtgagatgt
      661 cggcaggaac cgatgtccgc gcagggggga acttcctgca gtacctgacg cttcaaatgg
      721 tattaaccgt agggtgttgt tggttccgga tcgcggaaat gggagcacag gtctgctacg
      781 gggtgtacct ctgacagtat atccggctcg ctcatctgac agcctctctc ccggaaaaga
      841 ccaaaaatga gatcgtctcc tcaacagcag agaagaggtc ttgacagaca ggctacattg
      901 catacgattt atgaagaaaa agacagcaaa tatggaggtt atagcggaga ataccctacg
      961 aaattcctat gaaaaacgat tgaaaaaaat atcaaattcg attcgttttt atatgctttt
     1021 tgggagcgtg gcgatgacga actgttgcat gtgcagagga gaatgcaccc ggaagcacca
     1081 gaatcatcca gaaaggcaga acgattacgc atcaaaccag gagac
//
LOCUS       DAZFYC010000376         1123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFYC010000376 DAZFYC010000000
VERSION     DAZFYC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.383"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..174
                     /locus_tag="ACHKW5_005598"
     CDS             <1..174
                     /locus_tag="ACHKW5_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514723.1"
                     /translation="YCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFT
                     GICNDLAAASTFV"
     gene            138..713
                     /locus_tag="ACHKW5_005599"
     CDS             138..713
                     /locus_tag="ACHKW5_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514724.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPVFRQKLSDGWYQVRIAG
                     RDAGETELSSRLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIATAIWMPGISIA
                     FSDILFSMGAAMTLGGVAQMLAPKASTATTTSTDNGKQNTYFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVISQEISTADEGDGGEIVVIGR"
     gene            774..>1123
                     /locus_tag="ACHKW5_005600"
     CDS             774..>1123
                     /locus_tag="ACHKW5_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7514725.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEENTNISGVTVVFRSGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFG"
BASE COUNT          256 a          273 c          361 g          233 t
ORIGIN      
        1 tactgtggtg acggcgagct gctgcaccat attcctgaac aactgagcaa acgagagagg
       61 tataccgaca aatggcagcg acgcacacac tccctctggc gtcaccgggc atggcgcgca
      121 tctgccttta cggggatttg caacgatttg gccgccgcat cgaccttcgt gtgaaaacgg
      181 gggctgaagc catccgcgca ctggccacac agctcccggt gtttcgtcag aaactgagcg
      241 acggctggta tcaggtacgg attgccgggc gtgatgcagg cgaaaccgaa ttatcatccc
      301 gtcttaatga gccgctggca aatggtgcag tgatccacat cgtgccgcgt cttgcgggag
      361 caaaaagtgg cggtgtgttt caggcggtgc tgggtgcggc gttgattgct acggcaatct
      421 ggatgccggg gatcagtatc gctttcagtg acattctctt ttcaatgggt gcggcgatga
      481 cgcttggcgg tgttgcacag atgctggcac cgaaagcgag taccgcaaca acgaccagca
      541 cggataacgg taagcagaac acgtatttct catcactgga taacatggtt gcccagggca
      601 atgttctgcc tgttctgtac ggtgaaatgc gtgtggggtc acgggtaatt tcgcaggaaa
      661 tcagcacggc agacgaaggg gatggtggtg aaatcgtggt gattggtcgc tgatgcagaa
      721 tgttttatgt gaaaccgcct ccgggcggtt ttgtcgttta tggagcgtga ggaatgggta
      781 aaggcagcag taaggggcat accccgcgcg aagcgaagga caacctgaag tccacgcagc
      841 tgctgagtgt gatcgatgcc atcagcgaag ggccgataga aggtccggtg gatggattaa
      901 aaagcgtgct gctgaacagt acgccggtgc tggacagtga ggagaatacc aatatctccg
      961 gcgtcacggt ggtgttccgg tcaggtgagc aggagcagac accgccggag gggtttgaat
     1021 cctccggctc cgagacggtg ctgggtacgg aagtgaaata tgacacgccg ataacccgga
     1081 ccatcacgtc ggcaaacatt gaccgtctgc gcctgacctt cgg
//
LOCUS       DAZFYC010000377         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFYC010000377 DAZFYC010000000
VERSION     DAZFYC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.384"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1118
                     /locus_tag="ACHKW5_005601"
     CDS             <1..>1118
                     /locus_tag="ACHKW5_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7514726.1"
                     /translation="LSVVDTDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLPRMNFGSRSEKVSRRIAQMEADLNRLHKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTTEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIARPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPA"
BASE COUNT          241 a          320 c          344 g          213 t
ORIGIN      
        1 ctttccgtcg ttgacactga cgacatcttc ctgctgaaac agcgcctggc cgaacaggaa
       61 gcgctgatcc acgccctgca ggaaaagctg agcaaccggg agcgcgaaat agaccatctg
      121 caggcgcagc tggataaact cccccggatg aacttcggca gtcgttccga aaaagtctcc
      181 cgccgtatcg cacaaatgga agccgatctg aaccggcttc ataaagagag cgatacgctg
      241 actggtaggg tgtatgaccc ggcagtacag cgtccgttgc gtcagacccg cacccgtaag
      301 ccgttccctg aatcactacc ccgtgacgaa aagcgactgt tgcctgcggc gccgtgctgc
      361 ccgaactgcg gcggttcact gagctatctg ggcgaggata ccaccgaaca gctggagttg
      421 atgcgtagcg cctttcgggt tatccggacg gtacgggaaa aacatgcctg tactcagtgc
      481 gatgccatcg tgcaggcacc tgcaccttcg cggcccatcg agcggggtat cgccagaccg
      541 gggctgctgg cccgtgtgct gacctcgaag tatgcagagc acaccccgct gtatcgccag
      601 tcagaaatat acggccggca aggtgtggag ctgagccgtt cactgctgtc gggctgggtg
      661 gatgcatgct gccggctgct gtctccgctg gaagaggcgc ttcatggcta tgtcatgact
      721 gacggcaaac tccatgccga tgataccccg gtccaggtac tgctgccggg taataagaag
      781 acgaagaccg ggcggttgtg ggcgtatgtt cgtgatgacc gcaatgccgg gtcagcgttg
      841 gcacctgcag tgtggttcgc ttacagcccg gacagaaaag gcatccatcc gcagactcat
      901 cttgcttgct tcagcggtgt gctgcaagcg gatgcgtacg ccgggttcaa cgagctgtat
      961 cgcaatggtg ggataacgga agctgcctgc tgggctcatg cccgccgaaa gatccacgat
     1021 gtgcacgtcc gcatcccgtc agcactgacg gaagaagccc tggagcagat cggtcagttg
     1081 tacgccatag aggcggatat aaggggaatg ccggcaga
//
LOCUS       DAZFYC010000378         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFYC010000378 DAZFYC010000000
VERSION     DAZFYC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.385"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..219
                     /locus_tag="ACHKW5_005602"
     CDS             <1..219
                     /locus_tag="ACHKW5_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7514727.1"
                     /translation="ALFPGRIMLANTCMWTYRSDECGYNGPAVADEFDNPTTDIRKDR
                     CSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            230..973
                     /locus_tag="ACHKW5_005603"
     CDS             230..973
                     /locus_tag="ACHKW5_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7514728.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPG
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFCRVSLSRAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            871..>1112
                     /locus_tag="ACHKW5_005604"
     CDS             871..>1112
                     /locus_tag="ACHKW5_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514729.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SLQMPGFRRQMNEGWYQIRIRDEDTAPEAVYARLHE"
BASE COUNT          221 a          291 c          356 g          244 t
ORIGIN      
        1 gcgctgtttc ctggtcgcat catgctggcg aacacctgta tgtggaccta ccgctctgat
       61 gaatgcgggt ataacggtcc tgcggtggcg gatgagttcg acaaccccac cacggatatc
      121 cgtaaggaca gatgcagcaa gtgcatgcgc gggtgtgaga tgcgcggcat ggtggctaat
      181 tttggcggtt tcctttccat taataaactt tcgcagtaaa tcccggttta tgacacagac
      241 tgaatcagcg attctggcgc atgcccggcg gtgtgcgcct gcggagtcgt gcggcttcgt
      301 ggtgagaacg ccggaggggg agcggtatat cccttgtgtg aatatctctg cagagccggg
      361 ggcgtatttt cgtattgcac cggaagactg gctgcgggca gagatgcagg gggagattgt
      421 ggcactggtc cacagtcatc ccggtggtct gccctggctg agcgaggccg accggcggct
      481 gcagataaaa agtgcactgt cctggtggct ggtctgccgg ggggaaattc ataaattccg
      541 ctgtgtgcca catctgacag gacggcgctt tgagcacggg gtgacggact gttacacgct
      601 gttccgggat gcataccatc tggcgggaat tgatatgccg gattttgagc gtgaggatga
      661 ctggtggcgc aacggtcaga acctgtacct ggacaatatg gaggccaccg gcttttgccg
      721 ggtgtccctg tcccgtgcac aggcgggcga tatcctgctg tgctgctttg gcgcatcggt
      781 ggccaatcat gccgccattt actgcggcaa cggtgaactg cttcaccatc tgcctgaaca
      841 actgagtaaa cgggagaggt attctgaaaa atggcaacga cgaacgcatt ctgtctggcg
      901 tcaccgccac tggcacgcat ctgccttcac ggggatttgc aacgatttgg ccgccgcctc
      961 agcctgtacg tgaacacggc agcggaagcc atccgtgccc tgtcgctgca gatgccggga
     1021 ttccgccgtc agatgaacga aggctggtac cagatacgta ttcgcgatga ggacacggca
     1081 ccggaggcgg tgtacgcccg tcttcacgaa ca
//
LOCUS       DAZFYC010000379         1101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFYC010000379 DAZFYC010000000
VERSION     DAZFYC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.386"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1101)
                     /locus_tag="ACHKW5_005605"
     CDS             complement(<1..>1101)
                     /locus_tag="ACHKW5_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7514730.1"
                     /translation="LGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQ
                     ITATPHLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYY
                     FYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDL
                     AEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGR
                     LYIAGIGAGIENAPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKY
                     LTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSA
                     NQIEGDLVKTVGKAFPRDSRAPERWPSGTITVR"
BASE COUNT          232 a          320 c          273 g          276 t
ORIGIN      
        1 cctgacggta atggtccctg atggccaccg ttccggtgca cgggagtccc gggggaaagc
       61 tttgcccact gttttaacga gatcgccttc aatctggttc gcggacagtt ttcccagaac
      121 ccggcagttc tcgttaatcg tgacgttgtt gagcgtcccg gagtttgcat tcacgttacc
      181 gctgatatcg gcatttttcg ccgtcagccg cccgtccggt gtcagggaaa atgccggagg
      241 attaccgccg ctggtaatgg tgggagccgt cagatatttc aggaacactt cgttcatgaa
      301 tatctgatcg ccctgcccaa caaacatcgg ctttgtgttg ccattcgcag gattaatcat
      361 cgcaatcctg tccgccgcca gcagcacctg actctgcatt ccgtcaggag cattctcaat
      421 accggcacca atacccgcga tataaaggcg tccgtcctgc atctgctgca gcttcacagc
      481 ccacatgctg ttcaggttat tatttgtatc aacctgaacc ttctgtatct gctggatcgc
      541 tgcactctgg tcttccagtt tcttattgac ggtctgcgtg atttcattgc tgacatccgt
      601 aatggacgtc ctgatttcag ccaggtcagg cgcaagctga ccgttatcaa tctgcgtcca
      661 cagctcctga gccagatggg ttttccctat ctcgcctttg aaaaaatcca gatagccgga
      721 tgcatcatca ctcggctggc caacagcctc cacgaatgcc gatttgccaa cggtgttcac
      781 actgcggatg taaaaataat aatcatggcc cggtttgata ttgatactgg cggctatcca
      841 gtacagcgcc gtgccaagat agcgggctgt ggtttcaacc tgcctgatat cggtaatccg
      901 cgtttccgag aaccagaact caaactgtac cgtcggatca taaaccgcaa gatgcggcgt
      961 ggcagttatc tgaaaatagc ccggcgtcag ctcaatccgc gacggcgctg ccggtgcggc
     1021 aatccggaac gataccgatg ccggatcgcc ctgctgtccc caggcatttg ccgcccggac
     1081 tgtcagcctg tagtttccca a
//
LOCUS       DAZFYC010000380         1096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFYC010000380 DAZFYC010000000
VERSION     DAZFYC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.387"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..746
                     /locus_tag="ACHKW5_005606"
     CDS             30..746
                     /locus_tag="ACHKW5_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7514731.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(791..>1096)
                     /locus_tag="ACHKW5_005607"
     CDS             complement(791..>1096)
                     /locus_tag="ACHKW5_005607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514732.1"
                     /translation="SVFRALNRVSRFSEQVAPNRHHDSQGQKLCSMKYRTKIKRKMPY
                     AGCRYRVFIGKLCVIATLPSVIRKRRTGILINIISLGTGIFYNQYDELNIIFFIRVI"
BASE COUNT          356 a          198 c          225 g          317 t
ORIGIN      
        1 gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat attgttgcta
       61 aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt gtagaaatct
      121 atgctgctgc taaacaaggc gaacccgatc cagaattaaa cacatcttta aaattcgtta
      181 ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca attgataaag
      241 ccaaaggcgg cggagatgaa acgttcgtgc agggacgtta tgaaggcttt ggtcctaatg
      301 gctcaatgat tatcgctgag acattgactt caaatgttaa ccgtacgatt gctaacgttc
      361 gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc agctatatgt
      421 ttgacaatac gggtgtgatt gtatttaaag ggacagaccc tgaccatatt tttgaaattt
      481 tacttgaagc tgaagttgat gttcgtgatg tgactgaaga agaaggtaac attgttattt
      541 atactgaacc tactgacctt cataaaggaa tcgcggctct aaaagcagct ggaatcactg
      601 agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt tccccagaag
      661 atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat gtacaaaaag
      721 tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt tacggcagat
      781 ttctttaatc tcatataacc cttataaaaa atataatgtt caactcgtca tattgattat
      841 aaaaaatccc cgttcccaga gaaataatat ttattaaaat tccagttctt ctttttctga
      901 ttacagaagg caaagtggca ataacgcata gtttcccgat aaagacgcga tagcgacatc
      961 ccgcataagg catttttctc tttatctttg tacggtactt catggaacag agtttttgac
     1021 cttgcgaatc gtgatgtctg ttgggggcaa cttgctcact gaagcgtgag actcgattaa
     1081 gcgcacgaaa cacaga
//
LOCUS       DAZFYC010000381         1086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFYC010000381 DAZFYC010000000
VERSION     DAZFYC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.388"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..472)
                     /locus_tag="ACHKW5_005608"
     CDS             complement(<1..472)
                     /locus_tag="ACHKW5_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7514733.1"
                     /translation="MTQTESVILAHTRRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFVREDDWWRNGQNLYLDNM"
     gene            complement(478..>1086)
                     /locus_tag="ACHKW5_005609"
     CDS             complement(478..>1086)
                     /locus_tag="ACHKW5_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7514734.1"
                     /translation="QGGERYFFCNELNEKGEAVTWQGRKYQAYPIEGSGFEMNGKGSS
                     ARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELS
                     DRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWTYRSDECGYTGGAVA
                     DEFDKPTTDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
BASE COUNT          231 a          381 c          258 g          216 t
ORIGIN      
        1 ccatattgtc caggtaaagg ttctgaccgt tgcgccacca gtcatcctca cgcacaaaat
       61 ccggcatatc aattcccgcc agatggtatg catccctgaa cagcgtgtaa cagtccgtca
      121 ccccgtgctc aaagcgccgt cctgtcaggt gcggaacgca gcggaattta tgaatgtccc
      181 cccggcagac cagccaccag gacagtgcac tttttatctg cagccgccgg tcggcctcgc
      241 tcagccaggg cagaccaccg ggatgactgt ggaccagtgc cacaatctcc ccctgcatct
      301 ctgcccgcag ccagtcttcc ggtgcaatac gaaaatacgc ctccggctct gcagagatat
      361 tcacacaagg gatataccgc tccccctccg gcgttctcac cacgaagccg cacgactccg
      421 caggcacaca ccgccgtgtg tgtgccagaa tcactgattc tgtctgtgtc attggattta
      481 ctgcgaaagt ttattaatgg aaaggaaacc gccaaaattg ccgacattcc tgcgcagttc
      541 acacccgcgc atgcacttgc tgcatctgtc cttacggata tccgtggtgg gtttatcgaa
      601 ctcatccgcc acagccccgc ccgtgtaacc acactcatca gagcggtagg tccacataca
      661 ggtgttcgcc agcatgatac gaccgggaaa cagcgccccg tccgtctcgg tcggtgtggc
      721 cagcacaaac gaggctgtca tggctgtcag ctctgacatc tgctccacca cccagcggtc
      781 gctcagctcc tgctccgggt ccgcttccgg attgcccgcc acaaaattca ccgcatccag
      841 aaaacgggca tacacccggc ggcggaccac cgtggccccc accaggctct gcaggtcctc
      901 cgccatcccg gtgacaagac cgaacagatt ggacaccgtc agcgacgggc gggcactgct
      961 gccctttccg ttcatctcaa agccgctgcc ctcaatcggg tacgcctggt acttccgccc
     1021 ctgccaggtg accgcctccc ctttttcatt cagctcatta cagaaaaaat accgctcacc
     1081 gccctg
//
LOCUS       DAZFYC010000382         1054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFYC010000382 DAZFYC010000000
VERSION     DAZFYC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.389"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..315)
                     /locus_tag="ACHKW5_005610"
     CDS             complement(<1..315)
                     /locus_tag="ACHKW5_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO7514735.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFG"
     gene            496..648
                     /locus_tag="ACHKW5_005611"
                     /pseudo
     CDS             496..648
                     /locus_tag="ACHKW5_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(673..>1054)
                     /locus_tag="ACHKW5_005612"
     CDS             complement(673..>1054)
                     /locus_tag="ACHKW5_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514736.1"
                     /translation="KSSIRNIFYPSRLERLSRFQFIIYTLTSWSILITLNDINLALSF
                     ILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLN
                     VAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          341 a          179 c          202 g          332 t
ORIGIN      
        1 tccaaaaaag gaaaacattt ttgctagttc tttcatttct gcaaataaaa tatcagtgca
       61 ttcacgttgt gatatttttg ttccggaact tgaggcacta aaagcaacat taatagaacc
      121 tgggccaggg aaatggtatt taataagtct gtaaggatca acttttgctg agatatcgct
      181 taggccgagt gagagaaagt cttgttctac catatcacca tattgcatat catcagcaga
      241 aaaatcatcg aataaactaa tagtttcgaa aagtgtacag ggaatattaa ttggcggaag
      301 taatatgtta ttcatttgca tttgccttgg ttatatttaa gctcaatatc aattcgagaa
      361 caaatgctag ggtaaaaaaa tatttaaaaa agaagtaaac ttcacatttt ttcattacat
      421 ctctgaagag aacctcgtta atgattggtc ttgctcattg ttaataacaa aatgttgaat
      481 ttaccgatta ggaaactttt tcctacgcgg attgcctcca gctatggcat gaactttgag
      541 tttatgccag aactgaagtg gggcttcggc taccctggcg cgattatctt tatgatcctc
      601 gcgggcctga catcgtatct gtactttaag cgtaagaact ggctgtaact atacgatgtc
      661 gctgtgagta agttagtgtg ctgacagcga cattttagcc gagggaatcg cagataacat
      721 aattgttgtg aataatatcc atgccacgtt gagttggacg cttaatgact cacatctcac
      781 cccttcaatt gctgcagaga taatagaaaa aagtgtcaag cccaatgaaa tcaatacaaa
      841 gtagattgcc ttacatccag tatctcttat tctttttgct ccaatattga tatttacata
      901 ggtattatta ataaccagaa taaaactcaa agccaggttg atatcattca aagttattaa
      961 tattgaccat gatgttaatg tatagattat gaactgaaag cgtgataaac gctcaagcct
     1021 ggatgggtag aatatatttc tgatggatga tttg
//
LOCUS       DAZFYC010000383         1048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFYC010000383 DAZFYC010000000
VERSION     DAZFYC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.390"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..110)
                     /locus_tag="ACHKW5_005613"
     CDS             complement(<1..110)
                     /locus_tag="ACHKW5_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7514737.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNE"
     gene            complement(58..624)
                     /locus_tag="ACHKW5_005614"
     CDS             complement(58..624)
                     /locus_tag="ACHKW5_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7514738.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          329 a          230 c          164 g          325 t
ORIGIN      
        1 atctcattac accaatattg attttgatat tttggtataa accattcatt gcaacattta
       61 catttccttc gatagattcg cataagtgct cctttcgttg ccggaaaaat caccgtaata
      121 cttatctcgg gcttcttcag caactagtac cgctaactcc agatcatcaa agcatccgaa
      181 gtgtttactc ttgccatgga atcctagcct aacattccat tttttctgtc gtttgtgcca
      241 agtaacccct ctgcaacctg atttgctatt ctttcggatc cttatatttc ttgaattttc
      301 tattggcagg cattctctta aattttctgg cctattgtcg gtcctaattc cattaacgtg
      361 gtcaatttga ccagcaggcc aacgattatg agttatgtaa aaaactaaga cgtgagtttt
      421 atatctacgc ccatctatca tgatcattga ataaccgttg gaatcaaaag ttccagcaac
      481 actatttaat gctatccttc cctgagtggg aactttccat ctaaataccc cggtagattt
      541 atcgaaactt agtaactcaa atatcctttt aacagttaaa tcttctcttt tacggttaca
      601 tcgtcttcgc gctggtttag ccatcgcctt cttcctccgt aatggttttc tgaatttggc
      661 cacctgaaca gagctcacca aagctatgga tgtcggtatt tccacaatac caagatggcg
      721 aaaataactg catgataagc ctcagggaaa agggaagaca ctacccccga taattcagaa
      781 acaaatcgaa atacatgaac taaagaaaaa aatcacaaca aatagaaata gaaatagaaa
      841 tagaaataga aatagaaata ttaaaacaga ccaccacatt cctgatgtca tactcactaa
      901 caatttttcg acaacatggt agctcatagc acgttatcgt gtagacaccc tctgctacgt
      961 attcggtgtt cagtgcaata tctacaaata ctggaaaaat ctaaaatcga ggcaagtttt
     1021 tagtgataac tatagttaga ctatattg
//
LOCUS       DAZFYC010000384         1030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFYC010000384 DAZFYC010000000
VERSION     DAZFYC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.391"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..112)
                     /locus_tag="ACHKW5_005615"
     CDS             complement(<1..112)
                     /locus_tag="ACHKW5_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514739.1"
                     /translation="MCLHGDLQRFGRRLSLYVNTAAEAIRALSMQMPGFRR"
     gene            complement(58..801)
                     /locus_tag="ACHKW5_005616"
     CDS             complement(58..801)
                     /locus_tag="ACHKW5_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7514740.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVTGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFEREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            complement(812..>1030)
                     /locus_tag="ACHKW5_005617"
     CDS             complement(812..>1030)
                     /locus_tag="ACHKW5_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7514741.1"
                     /translation="ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDR
                     CSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          226 a          329 c          276 g          199 t
ORIGIN      
        1 gacggcggaa tcccggcatc tgcatcgaca gggcacggat ggcttccgct gccgtgttca
       61 catacaggct gaggcggcgg ccaaatcgtt gtaaatcccc gtgaaggcag atacgtgcca
      121 gtggcggtga cgccaggctg aatgcgttcg tcgttgccat ttttcggaat acctctcccg
      181 tttactcagt tgttcaggca gatggtgaag cagttcaccg ttgccgcagt atatggcggc
      241 atgattggcc accgatgcgc caaagcagca cagcaggata tcgcccgcct gtgcagagga
      301 caggggcacc cggtaaaaac cagtcgccgc catattgtcc aggtaaaggt tctgaccgtt
      361 gcgccaccag tcatcctcac gctcaaaatc cggcagcgtt atccccgcca gatggtatgc
      421 atcccggaac agggtgtaac agtccgtcac cccgtgctca aaacgacgcc cggtcaggtg
      481 cggcacacag cggaatttat gaatttcccc ccggcagacc agccaccagg gcagggcact
      541 ctttatctgc agccgccggt cggcctcgct cagccagggc agaccaccag ggtggctgtg
      601 aaccagcgcc acaatctccc cctgcatctc tgcctgcagc cagtcttccg gcgcaatacg
      661 aaaatacgcc tccggctctg cggagatatt cacgcagggc tggtaccgtt cgccctccgg
      721 ggtgcctgtc acgaagccgc acgactccgc aggcgcacac cgccgggcat gcaccagaat
      781 cgctgattca gtctgtgtca taaaacagga tttactgcga aagtttattg atggaaagga
      841 aaccgccaaa attagccacc atgccgcgca tctcacaccc gcgcatgcac ttgctgcatc
      901 tgtccttacg gatatccgtg gtggggttgt cgaactcatc cgccaccgca ggaccgttat
      961 acccgcattc atctccccgg taatcccaca tacaggtatt cgccagcata atgcgaccgg
     1021 gaaacagcgc
//
LOCUS       DAZFYC010000385         1028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFYC010000385 DAZFYC010000000
VERSION     DAZFYC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.392"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1028)
                     /locus_tag="ACHKW5_005618"
     CDS             complement(<1..>1028)
                     /locus_tag="ACHKW5_005618"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7514742.1"
                     /translation="FFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFR
                     QTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQ
                     SCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCK
                     RIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHGPTYYL
                     RYPESHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDTV
                     LLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSKKIDLNCLSEHA
                     LNRTVSHD"
BASE COUNT          323 a          192 c          163 g          350 t
ORIGIN      
        1 tatcatgact tactgttctg tttagagcat gttctgacaa acaattcaaa tcaatttttt
       61 tagaggaaac gaaccccgga gacatccagg taataagagg cacatgcgtt tgctcagctg
      121 gagcaacatt atatggtgtt ccatgtaagt ataatccatt ttctcccaga gattctccat
      181 ggtcagagac atataataaa acagtatcat actcatcttt atattcgata agcttgtcaa
      241 ttaattttga tattatataa tcagtatagc gaattgtatt atcataggta ttaatcaatt
      301 gctcatgcgt acaattttcg atatcactac gatcacatgt tggtttaaaa tatttatgtg
      361 actcaggata acggagatag taggtaggac catgactacc catcaaatga aatacgataa
      421 gtttatcctc tgagttatta tttatatact catcaatatt ttttagcata acctcatcat
      481 aacatgagtt tttcttgcat aatgtattat ctgaatttga tggctcaata ttaattgtag
      541 ggattcgctt acatacacct ttacatcccc cgtcattttc aatccagagt aaatttaccc
      601 ccgttctgta cagaatatcc agtacatttt ccgaatttac cgctttgcgg gaattgaact
      661 cttttcgctt catatctgaa aacatgcatg gaacagatat ggctgtggat gtgccacacg
      721 actgcacatt atgaaaggat ataagttcat tatatttctt tgtaaaatca ttggtaccac
      781 gagaatatcc attcagagca tagttttgac ttcttgctgt ctccccgaca actaaaaaca
      841 taattaccgg cttttgtttt tcatttattg tgcgggatgc atccgttcca gtctgacgaa
      901 actcgccagg agaaacaaaa aaatccctta catatttaaa cccactgtag atataattag
      961 taggtattat ttctttattt aatgaagagt tgtttctgcc aactgatgca taatccttat
     1021 aaaagaaa
//
LOCUS       DAZFYC010000386         1028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFYC010000386 DAZFYC010000000
VERSION     DAZFYC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.393"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            185..970
                     /locus_tag="ACHKW5_005619"
     CDS             185..970
                     /locus_tag="ACHKW5_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514743.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          334 a          194 c          209 g          291 t
ORIGIN      
        1 tttaatcata ctatcgaatc attcaattta aatatatttg aatgttatga ttttcatttt
       61 tctattgatt taatgaattt atatatactg ttatttttgt ttgttctgat tcaagtggag
      121 tatataatta aaaatcgtta gctaaacatt accatgatta tgacaggcga gggagtgtcc
      181 aattatgaat tcaaaaaagc tttgttgcat atgtgtgtta ttctcgctgc ttgcaggatg
      241 tgcctctgaa tcttctattg atgaaaagaa gaaaaaagct caagtcacac aaagtaatat
      301 taataaaaac actccccagc aactgacaga caaagattta ttcggtaatg aaaccactct
      361 ggccgtatcc gaagaggata ttcaagctgc gcttgatgga gatgagttcc gcgttcccct
      421 taattctcct gtaatacttg ttcaatccgg caaccgcgca ccggaaacca ttatgcagga
      481 ggagatgcga aaatattata ctgtttccac attctctggt atcccggaca ggcaaaagcc
      541 tctgacttgt aacaaaaaca aagataaaaa tgagaacgag gatgttgcca gtgctgagaa
      601 tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa gggcatcaga aagcgattat
      661 tgttttccag gatacgttgc agacaggaaa atatgactct gcgctgaaat caacagtatg
      721 gtccgactat aaaaatgaaa aactcaccga cgctatctcc ctgcgctacc tggtacgttt
      781 cacgctggtg gatgtggcaa caggtgagtg ggctacctgg tcgccggtga attatgaata
      841 taaagtgctt ccaccattgc ccgacaagaa tgaagccagt acgactgata tgacagagca
      901 gcaaatcatg caacttaagc aaaagaccta taaagcgatg gtaaaagatt tggttaaccg
      961 ctatcaataa taaattatat ctgccgccag gaatttctgg cggcaataat acaaaattct
     1021 ttgcataa
//
LOCUS       DAZFYC010000387         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFYC010000387 DAZFYC010000000
VERSION     DAZFYC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.394"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..453
                     /gene="etgA"
                     /locus_tag="ACHKW5_005620"
     CDS             <1..453
                     /gene="etgA"
                     /locus_tag="ACHKW5_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO7514744.1"
                     /translation="KIILSIILISNSCYASDCFEITGKAYNIDPLILKAIAWNESKNK
                     NGIKSKTNKNGTYDIGIMQINSSHLDLLSKFNISEDDLLNDACINISVAGYILASNIK
                     SRGNTWDAVGAYNAGYFNTPNAVELRRQYAMKIYKTYTKLKNNEQIID"
     gene            complement(450..>1026)
                     /gene="escU"
                     /locus_tag="ACHKW5_005621"
     CDS             complement(450..>1026)
                     /gene="escU"
                     /locus_tag="ACHKW5_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO7514745.1"
                     /translation="FMGHSYANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFS
                     VFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKS
                     TVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARS
                     LYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
BASE COUNT          362 a          176 c          166 g          322 t
ORIGIN      
        1 aaaattatat tgagtatcat tctcatttca aattcttgtt atgcttctga ttgttttgaa
       61 attactggaa aagcatataa tatagaccca ttaatattaa aagcaattgc atggaacgaa
      121 tcaaaaaaca aaaacggaat aaaaagtaaa acaaacaaaa acggaacgta tgatataggt
      181 ataatgcaaa taaattcatc tcatttagat ttattaagca aatttaatat atctgaagat
      241 gatttattga atgatgcctg tataaacata tctgttgctg gttatatctt agcctctaac
      301 ataaaatcca gggggaatac atgggatgca gttggtgcgt ataatgcagg ctactttaac
      361 acaccaaatg cagtagaact cagaagacaa tatgcaatga aaatatataa aacatacact
      421 aaattaaaaa acaatgaaca aattatcgat taataatcaa gatctattgc tatacgaatc
      481 aattgtgcta caggttcaaa aaagtcttct gttatgtatt gtcctttatg tatgttctta
      541 taaagacttc ttgccaaagg aatatcttca atcactggaa tatcatagag ttcagccagt
      601 ttaatgatct gtagagcttt ggcatctttt cctgtttcaa taactaaagg caatggagtc
      661 tccccgagtt tatagtatag gcaaatcgca atgtgagtcg ggtttttgac gatcacggtt
      721 gattttttga tgttattagc cagacttcca ctttgtatct cagaatgaag gcgacggcgc
      781 tcccctttaa tttcagggtt accatcagta tccttggctt ctcgtttcac ctcatcttta
      841 ctcattttca ttttcttcag tccctcatgc ttctggaacc agaaatcaaa tactgaaaag
      901 agtagatatc cgaataacac gcctttccat aaaagaaaaa caaaaaaggc aacaacgaca
      961 agagtttgat atgcgttcag tcttgtgaaa tttgcaaact catttgcata tgaatgcccc
     1021 ataaag
//
LOCUS       DAZFYC010000388         1022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFYC010000388 DAZFYC010000000
VERSION     DAZFYC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.395"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..757
                     /locus_tag="ACHKW5_005622"
     CDS             <1..757
                     /locus_tag="ACHKW5_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7514746.1"
                     /translation="DRLFEGINLDSAFSLSEEERSYFEEKAKSLNALAQLGISVEIIS
                     HELEEMDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRI
                     TGKNIMDYVLKFFGERFERQRITIEFSEEFKQIAITDIPSRIYPVFTNIINNAMYWVS
                     LSNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAV
                     AHHKIWYLEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            759..>1022
                     /locus_tag="ACHKW5_005623"
     CDS             759..>1022
                     /locus_tag="ACHKW5_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO7514747.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSD"
BASE COUNT          334 a          163 c          198 g          327 t
ORIGIN      
        1 cgatagatta tttgaaggta taaacttaga ttcagcattc tcattatctg aagaagaacg
       61 ctcatatttt gaagaaaaag ctaaaagttt aaacgcgctt gcacagttag gtattagtgt
      121 tgagataata tctcatgaac ttgaagaaat ggattctatg gtaaccagag gactaaactc
      181 tcttcctact tctgtaaaag aacaccctgg tttttcattg gcgttaaatg ctcacagatc
      241 gcttactcaa caaatacgtt tcttatcacc tttgaaaata tcaggttatc aatccaggca
      301 gagaataact ggaaaaaata tcatggatta tgtcctgaag ttctttgggg agcgtttcga
      361 acggcaacga ataactattg aatttagtga agagtttaag caaatcgcaa taacagatat
      421 accatcaagg atctatcctg tttttactaa tattatcaac aatgcaatgt attgggtcag
      481 tctgtcaaat aataggctca taaagattgg ttttgtgaat tctttggtta tcatagcaaa
      541 ttctggtccg gcaattgata ccgatgatat cccgcgacta tttgaactat tttatagcaa
      601 aagagcaaat ggacatgggg taggtctgta tctatgtcga gaaaaccttg ctgttgcaca
      661 tcataaaata tggtatttag aacctgatga aggcgataac tatttaataa aagatggcgc
      721 taattttgtg atccagttca atggagtgga gttctaatat gacagtggca aattataatt
      781 ctcttgtcca gaaaactttc tgcgaaaatg caattcgttc cgttgtcatg attgatgacg
      841 attttctgac gtattctgaa tcaatcaggg cgttgaataa cgaagttgat ttagactaca
      901 acaaaattga ctcatctaaa cgagccgcta ctcttgagag cttttttcaa tctaaaaata
      961 tgatttgtga tgttgacaat ggttctgtta atttcgatgt ggatcggatt agaaaatcag
     1021 at
//
LOCUS       DAZFYC010000389         1010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFYC010000389 DAZFYC010000000
VERSION     DAZFYC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.396"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..302)
                     /locus_tag="ACHKW5_005624"
     CDS             complement(<1..302)
                     /locus_tag="ACHKW5_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7514748.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEI"
     gene            complement(308..1006)
                     /locus_tag="ACHKW5_005625"
     CDS             complement(308..1006)
                     /locus_tag="ACHKW5_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7514749.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLAVSNLYGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
BASE COUNT          209 a          349 c          235 g          217 t
ORIGIN      
        1 tgaatttccc cccggcagac cagccaccag gacagtgcac tttttatctg cagccgccgg
       61 tcggcctcgc tcagccaggg cagaccaccg ggatgactgt ggaccagtgc cacaatctcc
      121 ccctgcatct ctgcccgcag ccagtcttcc ggtgcaatac gaaaatacgc ctccggctct
      181 gcagagatat tcacacaagg gatataccgc tccccctccg gcgttctcac cacgaagccg
      241 cacgactccg caggcacaca ccgccgggca tgcgccagaa tcgctgattc tgtctgtgtc
      301 attggattta ctgcgaaagt ttgttaatgg aaaggaaacc gccaaaatta gccaccatgc
      361 cgcgcatctc acacccgcgc atgcacttgc tgcatctgtc cttacggata tccgtggtgg
      421 ggttgtcgaa ctcatccgcc accgcaggac cgttataccc gcattcatct ccccggtaat
      481 cccacataca ggtgttcgcc agcatgatgc gaccgggaaa cagcgctccg tccgtctccg
      541 tcggtgttgc cagcacaaac gaggctgtca tggccgtcag ctctgacatc tgctccacca
      601 cccagcggtc gctcagctcc tgctccgggt ccgcttccgg attgcccgcc acaaaattca
      661 ccgcatccag aaaacgggcg taaaccttac gccggactac cgttccgccg accagactct
      721 gcatatcttc caccataccg gtgaccatgc cgtacaggtt agagactgcc agcgtgggcc
      781 gcgtactggt gcctttgcca ttcagttcaa aaccgctccc ctgaatgggg tatgcctgat
      841 actgtcgccc ctgccaggtg accggctcac ctttttcgtt ctgctcatta cagaaaaaat
      901 aacgttctcc accaacctct gtcagatcga tttcccagag caccacgctg gccgactgct
      961 ccgcacgggt gcattcattc agtgtttcct gccggatatc ctgcatcagt
//
LOCUS       DAZFYC010000390         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFYC010000390 DAZFYC010000000
VERSION     DAZFYC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.397"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            133..357
                     /locus_tag="ACHKW5_005626"
     CDS             133..357
                     /locus_tag="ACHKW5_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7514750.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLINEKHAQFVPNKDKHDQSTCSDRFT"
     gene            complement(439..753)
                     /locus_tag="ACHKW5_005627"
     CDS             complement(439..753)
                     /locus_tag="ACHKW5_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7514751.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          326 a          249 c          157 g          272 t
ORIGIN      
        1 tgtgtgtacg ccatcaaacc acgagaaata atcaattatt acgcaggtat cgtattaatt
       61 gatctgcatc aacttaacgt aaaaacaact tcagacaata caaatcagcg acactgaata
      121 cggggcaacc tcatgtcaac gaagaacaga acccgcagaa caacaacccg caacatccgc
      181 tttcctaacc aaatgattga acaaattaac atcgctcttg atcagaaagg ttcaggtaat
      241 ttttcagcgt gggttattga agcctgcaga agaagattaa ttaatgaaaa acatgctcaa
      301 tttgtaccca acaaagacaa acacgaccag agcacctgtt cagacaggtt tacttaaacg
      361 acttatatat gacacaaaaa gcgaccacta aagtcgcttt ttcttatggt aacaggcaat
      421 aacgctctca gatatttttt agcatttttt tgaccgcgcg tttccggacg tattctgttc
      481 tcctgtccct ttatatcgtc ggaatacccg ccgctcttca aatcccattc ccaactcaga
      541 atgtagtctg ttgaccgctt gttttatttc ggtcaggttc accggtgaaa ccggagtccg
      601 gcgcgcctta cgcaaacact ctgctcgttt ctgtgccgcc acttttcttt tctggtcatc
      661 acttagctgt accatcactt ttgcccatcg ttcagctgct ctccggtaca gtcctttttt
      721 ctccagacat tctgccacgt gatcatgtag cataagtgac ctccgattat ctacagactg
      781 ccatcctgaa tttaccttcc cttaatgaaa taacaataaa aaacaaacca cgcaaaaaca
      841 ataaaacaac acacaaaaaa aactaaataa taaacaaaaa taatcacctt attttattat
      901 tttttgaggg ggcaattact gaacaaaaaa cgctgactat atactcaaaa ccaaacaact
      961 attctgccaa tcaggtatca tggcaacaca cggaattacc gtgt
//
LOCUS       DAZFYC010000391          998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFYC010000391 DAZFYC010000000
VERSION     DAZFYC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.398"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            745..879
                     /locus_tag="ACHKW5_005628"
     CDS             745..879
                     /locus_tag="ACHKW5_005628"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514752.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT          309 a          192 c          200 g          297 t
ORIGIN      
        1 gaaataatga tctggcagcc tgagttcaca gataaaatac tctccaggaa acccggggcg
       61 gttcacccct aattcaacta attggaggca ttaccatgca ttagtgcaag cgccttgtgg
      121 attcttttgc taatgacgga aacatattgt acaatattgc atttatacct caataaataa
      181 atgagttatc agattgcaaa aatctgttat caaactaatt caaataaaaa gagattgata
      241 cgaccaacgt acacaaaaat tttcgatatt cgaactaaat aacagctcct aataccgcta
      301 cactttagct atccagtctg cgcctcagaa atggacgatg ccgttgaagg actggtgtat
      361 ggcaatgagc cgctttatta tcgaattcgg tgaccgcctg gacggtcact tcggagaaaa
      421 ggcatttaca cagcatccta aacaggttca gcataggtga tataccgcct tttcattttc
      481 ttctccatta tttataaggg aggtgtatta ttatgcaata ttattggttt tacccattgt
      541 tttttcagtt catgaatgtt tgcaaaaaaa taaatgagca acaattttat gtgtttaaaa
      601 taaattaatt ttacttctgt aatggtgtca tgattagcag ctttttaaac taatatcgac
      661 tttcaataaa taaggacagg taatggacaa caaaaatatc tgaacccggg tgagattcat
      721 aagttgcaca gtgctgtctt taaagtgcca cacccggaac gtaatcactg cctgctcctt
      781 atggggtatc ttcatggtgt ccaagcatca gagttattag gtataaaact gtctgatatt
      841 gatttgcagg ccggtaacct gaatatccgg cggctctaaa cggtttgtca actcagcccc
      901 tcggctgaaa caggaggtac agtccattcg tcgctggctg aaacttcggg atgaatgggt
      961 agaagaggga tatgaatggt tatttaacct ctatggca
//
LOCUS       DAZFYC010000392          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFYC010000392 DAZFYC010000000
VERSION     DAZFYC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.399"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..261
                     /gene="yddL"
                     /locus_tag="ACHKW5_005629"
                     /pseudo
     CDS             1..261
                     /gene="yddL"
                     /locus_tag="ACHKW5_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            300..>992
                     /locus_tag="ACHKW5_005630"
     CDS             300..>992
                     /locus_tag="ACHKW5_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7514753.1"
                     /translation="MITDLILHNNPRMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLH"
BASE COUNT          348 a          196 c          166 g          282 t
ORIGIN      
        1 gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat
       61 aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact
      121 gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg
      181 caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac
      241 gatgaagcca acggttcgcg cggcaacaat ctctgattga gttaagtgcc catcacaata
      301 tgatcactga ccttatatta cacaataatc ccaggatgaa aacaatcact ttaaacgaca
      361 accatattgc acatttaaac gccaaaaaca ctacagaact ggaatattta aacttaagca
      421 ataacaattt actgccaacc aatgacattg atcaactaat atcatcaaaa catctttggc
      481 atgtattagt taacggcatc aacaatgatc cacttgcaca aatgcagtac tggactgcag
      541 taagaaatat aattgatgac actaatgaag tgaccattga tttatcagga cttaatttaa
      601 ccactcaacc accagggctg caaaacttca cctctatcaa tcttgataat aaccaactca
      661 cacattttga tgcaaccaac tacgatagac tcgtaaagct aagtctgaat agtaatactc
      721 ttgagtcaat aaattttcct caaggcagaa atgtaagtat tacacatata tctatgaata
      781 ataatgctct cagaaatatt gatatagata ggctttcatc agttacttat tttagtgcgg
      841 cacataatca actagagttt gtgcaattag aatcttgcga atggctgcaa tacctgaatc
      901 tcagccataa tcaattaact gatattgttg caggtaataa aaatgaactc ttactgctgg
      961 atctatccca taataaacta acaagtttac ac
//
LOCUS       DAZFYC010000393          976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFYC010000393 DAZFYC010000000
VERSION     DAZFYC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.400"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>976)
                     /locus_tag="ACHKW5_005631"
     CDS             complement(<1..>976)
                     /locus_tag="ACHKW5_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7514754.1"
                     /translation="LGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQ
                     ITATPHLAVYDPTVQFEFWFSEKRITDIRQVETTARYLGTGLYWIAASINIKPGHDYY
                     FYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASR
                     LDEFSKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRI
                     AFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNA
                     DISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTS"
BASE COUNT          203 a          283 c          236 g          254 t
ORIGIN      
        1 tgctcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcaccttca atctggttgg
       61 ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg agcgtccctg
      121 agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt ccgtctgatg
      181 tcagggaaaa taccggaggg ctgccaccgc tggtaatggt gggggctgtc aggcgcttca
      241 ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc
      301 cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagcttgc
      361 cttcctccgt gtcctccatg ctgagaccaa tacccgcgac ataatgtttg ccgtctttgg
      421 tctgctcaat tttgacgccc cacatggcat tccatttatc gttagcgtcc ttccactctt
      481 tcgaaaactc atccagtctg ctggcgttat cctccgtcag atcaactttt tccagcagct
      541 ctttaccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa ccttccgcat
      601 catcgctcgc ccgaccgacg gcctccacga atgccgattt gccaacggtg ttcacactgc
      661 ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct atccagtaca
      721 gccccgtgcc aaggtagcgg gctgtggttt caacctgcct gatatcggta atccgctttt
      781 ccgagaacca gaactcaaac tgtaccgtcg gatcataaac ggcaagatgc ggcgttgcgg
      841 ttatctgaaa atagcccggc gtcagctcaa tccgcgacgg tgctgccggt gcggcaatcc
      901 ggaacgatac cgacgccgga tcgccctgct gcccccaggc atttgccgcc cggactgtca
      961 gcctgtagtt ccccag
//
LOCUS       DAZFYC010000394          963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFYC010000394 DAZFYC010000000
VERSION     DAZFYC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.401"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            400..963
                     /gene="nleG7"
                     /locus_tag="ACHKW5_005632"
                     /pseudo
     CDS             400..963
                     /gene="nleG7"
                     /locus_tag="ACHKW5_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
BASE COUNT          323 a          205 c          143 g          292 t
ORIGIN      
        1 cgtcattgca atcgacctgc gctatcgctg ccgcagttct tccagtttct gcatcgataa
       61 ctccatcttc tgagcaataa acctcaccta tgcaatattt tatgtcaatt atttcaattg
      121 cattaatatc ttgaatggat tacatagagt taatgtatcc agtactccct attctcttgc
      181 atataataca tgttgcaact tacatctcag cgctatgaaa aaacaccacc tctctcttta
      241 tgaaattctg gatttaccaa gcgctaattt atcatttcaa tctaccttta agcattgcat
      301 ttatctccct acgaggtcat actttaggaa gttaaatatg aatgataata tacctacagc
      361 gcgaaatcac aaacaatcga cttgtattac agaaaaaaca tgcctatctt tttaaacttc
      421 acagcaggca gtatcttacc tgagaatgag ctagcatctt tacgttatat tgtgcagcaa
      481 aatcaaaatg atactgtaat cataaaagaa cgttataaaa tggatatccg ttatatcgaa
      541 tcagtcaatg gttttacagt aaatcctgta tgcagtaatc atttctccat atttatggcg
      601 agacaaaaca ctattgctcg caacctggaa cagcagatca acaacggacg aagttttgca
      661 caaatatctc aggattttat gcttcaatta tcttcaaata taggatggaa aaaaaggggc
      721 cgaaaacgcc cttaaaaata aaatccattc tcattcattt gttgtaaatc ctgatgaatt
      781 ctcttgcgac acacaatttc ttaagtgccc aataacatta tgcgttccag aaaaaggggt
      841 ttttgtcaag aacgcactga actccaacat atgcactctt tatgataagt ctgcgttcat
      901 gaatctcaca agagaacatc taccccaccc tctcagcagg gaaaagatag taaaagaaat
      961 gat
//
LOCUS       DAZFYC010000395          946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFYC010000395 DAZFYC010000000
VERSION     DAZFYC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.402"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHKW5_005633"
     CDS             <1..112
                     /locus_tag="ACHKW5_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514755.1"
                     /translation="LESLSQIRAGRQEWERRLAAMVSRRRGKPGFKLARF"
     gene            112..>946
                     /locus_tag="ACHKW5_005634"
     CDS             112..>946
                     /locus_tag="ACHKW5_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7514756.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DG"
BASE COUNT          210 a          203 c          319 g          214 t
ORIGIN      
        1 gctggaaagc ctttctcaga tccgcgccgg acgtcaggag tgggaacgca ggcttgccgc
       61 gatggtgagc cgcaggcggg gaaaaccggg atttaaactg gcgaggtttt aatggcaatt
      121 attgatgatg tgatcggcgt gttttccccc gggtggaaag cagccagact gcgttcaagg
      181 gcgttaatca tggcctatga ggcggtgaaa ccgacccgga cacataaagc ccggcgggaa
      241 aatcgctctg ctgatcagct cagtaaatac ggtgcggttt ccctgcggga gcaggcccgt
      301 tttctggata tcaatcatga cctggtgatt ggtgtgtttg acaagctgga agagcgggtg
      361 attggtgcca ggggaattat tgtggagcct cagccattac gaaaaaacgg ggaaatggcg
      421 gctgagctgg ctgcggatat ccgccgtttg tgggctgaat ggtccgtgag tccggatgtg
      481 acagggcagt atacccgtcc tgtgcttgaa cgtttactgc tgcggacctg gctgcgggat
      541 ggtgaagtgt ttgcgcagat ggtcagtggt gcgggaaacg gtctggaacg gacggcggga
      601 gtgccattct ggcttgaggc gatggagccg gattttgttc ccatgcgcac tgatgaatcc
      661 gccggactga atcagggggt ttttcttgat gagtggggaa gaccgaaaaa atatctggtt
      721 tataaaaatt atccggtcag cggccggcag agtgatacga aagaaatcgc tgccggaaaa
      781 atgatccacc tgaagttcac tcgtcgtctg catcagacgc gaggctcatc catgttatcg
      841 ggggtgctga tgcggatcag tgcccttaag gagtatgagg atgcggaact gacagcggcg
      901 cgtattgctg cggcgctggg actgtatatc cgtaaaggtg acggac
//
LOCUS       DAZFYC010000396          944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFYC010000396 DAZFYC010000000
VERSION     DAZFYC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.403"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..192
                     /locus_tag="ACHKW5_005635"
     CDS             <1..192
                     /locus_tag="ACHKW5_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7514757.1"
                     /translation="PGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCY
                     GQVSRRDQESALACWGIDR"
     gene            352..489
                     /locus_tag="ACHKW5_005636"
     CDS             352..489
                     /locus_tag="ACHKW5_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514758.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMVSGYFIGQIRW
                     W"
     gene            711..896
                     /locus_tag="ACHKW5_005637"
     CDS             711..896
                     /locus_tag="ACHKW5_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7514759.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          238 a          211 c          273 g          222 t
ORIGIN      
        1 cccggtaagt gtttcccgtc gacgttttat aaacgaatta atgcaggcga tcgcaggggg
       61 gcgtgtgagg cgattcgctg gtggattaag gacggtggca gagactgccg tattcgctca
      121 aacaactgtt acggtcaggt atcccgtcgc gaccaggaga gtgcgctggc gtgctgggga
      181 attgacagat aagcagaata ttttgctgaa aaatgcggtt tgctcacacg gacggataac
      241 acgaaatcct gcgaactgac aaaaactaag tgaataaaag taaaaacccc gtttgttggc
      301 tgcaagcggg gttttgtgtt tcctgactct ggaaaagtca aaggagaaag tgtgtttgat
      361 tttagcaaac tgattcggga gattcgagtg atggctgaaa aattatctac ctggaagttc
      421 attcttatct ggctggtgtt tgtgattatg gtctccggtt atttcatcgg tcagatacgc
      481 tggtggtgaa atgaaccgcg tactgtgtgt ggtcatcatt gccctgctgg tggcctgtgg
      541 tgcgcttagt ctggggctga atcattaccg tgataacgcc atcacctaca aagcgcagcg
      601 cgataaaaaa gccagagagc tggagctggc aaacgcaacc attactgata tgcaggtgcg
      661 ccagcgcgat gttgctgcgc tcgatgcaaa atactcaagg gaattagctg atgcgagagc
      721 tgaaaatgaa actctgcgtg ctgatgttgc cgctggtcgt aagcgcctgc ggatcaacgc
      781 cacctgctcc ggtaccgtgc gtgaagccac cggcacctcc ggcgtggata atgcaaccgg
      841 cccccgactg gcagacaccg ctgaacggga ttatttcatc ctcagagaac ggttgatgac
      901 aatgcagaag cagctggaag gggcacagga ctatatccgc actc
//
LOCUS       DAZFYC010000397          941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFYC010000397 DAZFYC010000000
VERSION     DAZFYC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.404"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..572
                     /gene="escR"
                     /locus_tag="ACHKW5_005638"
     CDS             <1..572
                     /gene="escR"
                     /locus_tag="ACHKW5_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO7514760.1"
                     /translation="VIGTSFLKISIVLGILKNALGIQQVPPNMALTSVSLILTMFIMS
                     PIILQINDNISQEPINYTDSDFFQKVDEKILSPYRGFLEKNTEKENVEFFERAAQKKL
                     GNETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSP
                     VTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            572..841
                     /gene="escS"
                     /locus_tag="ACHKW5_005639"
     CDS             572..841
                     /gene="escS"
                     /locus_tag="ACHKW5_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO7514761.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            841..>941
                     /locus_tag="ACHKW5_005640"
     CDS             841..>941
                     /locus_tag="ACHKW5_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7514762.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLL"
BASE COUNT          278 a          156 c          174 g          333 t
ORIGIN      
        1 ttgttattgg tacttcattc ctgaaaattt cgattgtatt ggggattctg aagaacgcat
       61 taggtattca acaggtacca ccaaatatgg cgctaacatc agtatctttg atactgacaa
      121 tgtttattat gtctccgata atattacaga taaatgataa tatttctcag gaaccaatca
      181 attacaccga ctctgatttt tttcaaaaag ttgatgaaaa aatattatca ccataccgcg
      241 gttttttaga aaaaaataca gagaaagaaa atgttgagtt ttttgaacgt gcagctcaaa
      301 aaaaattggg taatgaaact atcttaaaaa aagactctct atttatactg ttaccagctt
      361 tcacgatggg gcagcttgaa gctgcattca agataggttt tttgctctat ttacctttta
      421 ttgcgataga tttgatcatt tccaatatct tattggcctt gggtatgatg atggtatcgc
      481 cagtaacaat ttcgattcct tttaagatct tgttattcat tcttgttgga ggctggcaaa
      541 aattattcga atttctgttg gtggtgaatt gatggaaact ggatattttg ttcaattatg
      601 tgtgcaaacg ctttggataa tatttatcct ctcactgcct acagtcatag cggcctctgt
      661 tgtcggcatt attattagcc tggttcaggc tataacgcag ttacaggatc aaacattgcc
      721 ttttttgcta aaaataatag cagtgtttgc tacgcttgcc ctgacttatc actggatggg
      781 aacaacaatc atcaacttct cgtcgataat atttgaaatg attccgaagg tgaatggcta
      841 atgaatgaaa taatgacggt catagtatca tcattttttt gtatactcag gccgctgggg
      901 atgtttatta ttctaccaat attttcaaca ggtgttttgc t
//
LOCUS       DAZFYC010000398          939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFYC010000398 DAZFYC010000000
VERSION     DAZFYC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.405"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(362..>939)
                     /locus_tag="ACHKW5_005641"
     CDS             complement(362..>939)
                     /locus_tag="ACHKW5_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO7514763.1"
                     /translation="FPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSD
                     YKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVF
                     CKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVF
                     TCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          344 a          199 c          125 g          271 t
ORIGIN      
        1 atcaattatc acattcaaaa aaaatactga caacaaagat actcttcaga caaagttgtc
       61 atatgtaaat tttcccttaa aacgttgctc aaaaaaaata taaaaatcat atgccctaat
      121 caacatcctc aagcactacg atatatgcag tatcctattc atatattacc accatatcag
      181 acaaatcgaa tcacaacgag ttctatcgta ctctttcatc ttcgacactg taaattttaa
      241 gaacattcgg ctcatgccac taaaccaaga cataataaca acggagcaat aaatgttccc
      301 actgaacatg tgcaaaccta aaaagacagt gttacaataa tattcatcca tggtctctaa
      361 atcaacgcca cgcaacagga taatacaatg aaaatatttc aggaaaagat tcttttgcat
      421 gctccgttag catatcagtc agcacagcgg tacaagtaaa aacattattt cttccttgta
      481 gcctgtctaa aatatcattg taataataaa ctgagccaaa tacctgagga tctgttttat
      541 aagcttgttc aatcaatcca tttgcatatc tccgtagaat ctcaggagaa tcatactctg
      601 taccaaacat atcactcgat gagtatttac agaaaacccc ccctaaacaa aataatattt
      661 cagcctgttt tcttagtggc atatctttca atacatggct ccccataatc atatctacat
      721 gctgagcgtt cagttcccaa ccatcaagaa agggattcca gacacaggca agtttccttt
      781 gctgatcatc agcaatcatt ttataatctg atttattgga attaagtgcc tcaataaatc
      841 tctctttgag ttcattatca gaaatgagta tatccagaat accagaaacg aaattctggg
      901 aaaaagcttt atgataagga atactgaaaa ttggaaatg
//
LOCUS       DAZFYC010000399          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFYC010000399 DAZFYC010000000
VERSION     DAZFYC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.406"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..81)
                     /locus_tag="ACHKW5_005642"
     CDS             complement(<1..81)
                     /locus_tag="ACHKW5_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514764.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSEL"
     gene            367..573
                     /gene="ybcW"
                     /locus_tag="ACHKW5_005643"
     CDS             367..573
                     /gene="ybcW"
                     /locus_tag="ACHKW5_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO7514765.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(738..>931)
                     /locus_tag="ACHKW5_005644"
     CDS             complement(738..>931)
                     /locus_tag="ACHKW5_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7514766.1"
                     /translation="STKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEAC
                     RRRLTSEKIAYTSIKSDEE"
BASE COUNT          265 a          169 c          203 g          294 t
ORIGIN      
        1 tagttcagaa taaaacaatt cacagtttaa atcttttcgc acttgatcga atatttcttt
       61 aaaaatggca acctgagcca ttggtaaaac cttccatgtg atacgagggc gcgtagtttg
      121 cattatcgtt tttatcgttt caatctggtc tgatctcttt gtgttttgtt gatgatttat
      181 gtcaaatatt aggaatgttt tcaattaata gtattggttg tgtaacaaag tgcggtcctg
      241 ctggcattct ggagggaaat acaaccgaca gatgtatgta aggccaacgt gctcaaatct
      301 tcatacagaa agatttgaag taatatttta accgctagat gaagagcaag cgcatggagc
      361 gacaaaatga ataaagaaca atctgctgat gatccctccg tggatctgat tcgtgtaaaa
      421 aatatgctta atagcaccat ttctatgagt taccctgatg ttgtaattgc atgtatagaa
      481 cataaggtgt ctctggaagc attcagggca attgaggcag cgttggtgaa gcacgataat
      541 aatatgaagg attattccct ggtggttgac tgatcaccat aactgctaat cattcaaact
      601 acttaacctg tgacagagcc aacacgcagt ctgtcactgt caggaaagtg gtaaaactgc
      661 aactcaatta ctgcaatgcc ctcgtaatta agtgaattta caatatcgtc ctgttcggag
      721 ggaagaacgc gggatgttca ttcttcatca cttttaattg atgtatatgc tatcttttct
      781 gacgttagcc tccgacggca ggcttcaatg acccaggctg agaaattccc ggaccctttt
      841 tgatcaagag cgatgttaat ttgttcaatc atttggttag gaaagcggat gttgcgggtt
      901 gttgttctgc gggttctgtt cttcgttgac a
//
LOCUS       DAZFYC010000400          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFYC010000400 DAZFYC010000000
VERSION     DAZFYC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.407"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(310..582)
                     /locus_tag="ACHKW5_005645"
     CDS             complement(310..582)
                     /locus_tag="ACHKW5_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514767.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            742..>923
                     /locus_tag="ACHKW5_005646"
     CDS             742..>923
                     /locus_tag="ACHKW5_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7514768.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDG"
BASE COUNT          257 a          166 c          227 g          273 t
ORIGIN      
        1 aacctgtatt tcaaaatcag agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg
       61 aataaacaat acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag
      121 gtaagaaaag gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc
      181 gctttctctt tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac
      241 agctgtgcaa gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg
      301 attttatggc tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca
      361 tgcgtaacga gaatcccata agcagggagg acttaattct tcattaaccc atgcgttgat
      421 attatgtttc agccgttgaa gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt
      481 tttatgtgag atcttaccgt aagggtctgc aagaatgctg cttgttgctt cgttattatc
      541 tgccatcaga agaagtaact ctgatttaac gttttctgtc attagttgta aaaatcttct
      601 gcgcaaactt tctttactgt tcatttatat ggcttcattt gttgtaatct gctgcgtctc
      661 aagggatatg tttatgagag cgaccatgag tgttggatta tatacctaac atatcaaggg
      721 attagaaatc gataaatccc catgaacgaa aaaataaaat acggcctgtc ggctgccgtt
      781 ctggcgctga ttggtgcagg tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa
      841 aaggaaggta accacaccac ggcataccgt gatggtgcgg ggatctggac catctgccgt
      901 ggtgccattc tggtggatgg taa
//
LOCUS       DAZFYC010000401          921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFYC010000401 DAZFYC010000000
VERSION     DAZFYC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.408"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..651
                     /locus_tag="ACHKW5_005647"
                     /pseudo
     CDS             19..651
                     /locus_tag="ACHKW5_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(670..921)
                     /locus_tag="ACHKW5_005648"
                     /pseudo
     CDS             complement(670..921)
                     /locus_tag="ACHKW5_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
BASE COUNT          302 a          144 c          181 g          294 t
ORIGIN      
        1 tacactcagg atgaatatat gggaaaaatt aaaattgtgg tttcagatca gcagccgttt
       61 atgattgatg ggataattgg atttctcgga cattatcctg atttatataa ggttgttggg
      121 ggctataaag atctgaagaa agctatagcg gagtgtaaca aatctacagc ctaaatattt
      181 attttaggag aatttgcagg tggaatgatg gggtccgaac tcgtaaaatg ggttaaatcg
      241 cataagatag atgctcatat tattacattt gtagcaaaga tgccgtatat tgattcaata
      301 aaattgcttg aagcaggtgc gaaaggatgc gtatggaaaa ccagtcaccc ggcgaaacta
      361 aatcgtgcta ttgattcgat tagtaatggt tacacttatt ttgatagtgt acatatggat
      421 tgtgaaaaaa tatcttccag gtattcctct gataatcaac ttacaaatcg tgagtcagaa
      481 atattacaac tcatagctga tggtaaaaca aacaaagaaa tcgctaattt cttgcagtta
      541 agcagaaaaa cagtcgaaac tcatagactc aatatcatga agaaattaga tgttcacagt
      601 ggtatcgagt tgatcaaaac agctttacgt atgggtgttt gcactatata aaaaaaagag
      661 gctttaggct cattttgctg aagccttacg attgtctagt tcattaagcc tggtatatac
      721 aatatatatt ttggggcata ttgctgtctc tttttttggt tatccattgc aaaacccgca
      781 ttataagtcc ccaggcattg ccacgttact ccacaacgtg aaaaatggtt atataaaatc
      841 tcggttccta ttttaatgtt cagacaaggg ttatctaaca aatctcgttc atcttttatt
      901 attccacgtt tttttaacag g
//
LOCUS       DAZFYC010000402          918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFYC010000402 DAZFYC010000000
VERSION     DAZFYC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.409"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..783
                     /locus_tag="ACHKW5_005649"
     CDS             <1..783
                     /locus_tag="ACHKW5_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7514769.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPADEATSY
                     AIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALR
                     VSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
     gene            767..>918
                     /locus_tag="ACHKW5_005650"
     CDS             767..>918
                     /locus_tag="ACHKW5_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO7514770.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDA"
BASE COUNT          224 a          204 c          294 g          196 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga taccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat cccacgcctg cggatgaagc cacgtcgtat
      481 gccatccgtt ttcctgatga tccggagatt ttttcgcaga cagaggcgca gcaactggtg
      541 gcggaagagc tggtggagaa gtgggaaaaa ggaaagatgc gtctgctgtg ggataacaaa
      601 aagcggcgta acgaagcgct ggactgcctg gtgtatgcct acgcggcatt acgtgtgtcc
      661 gtgcaacgct ggcagcttga tctggctgta ctggcaaaat cccgggaaga agagacgacc
      721 cggccaaccc tgaaagaact ggcagcgaag ctgtccggag gagtgaatgg ttacagtcgc
      781 tgaactgcag gcgctgcgtc aggcgcgcct tgatttatta accggtaaac gggtggtgtc
      841 tgtccagaaa gatggtcgca gaattgaata tacggcggct tctctggatg agcttaaccg
      901 ggcgatcaat gatgcgga
//
LOCUS       DAZFYC010000403          912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFYC010000403 DAZFYC010000000
VERSION     DAZFYC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.410"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..117)
                     /locus_tag="ACHKW5_005651"
     CDS             complement(<1..117)
                     /locus_tag="ACHKW5_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514771.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNA"
     gene            complement(114..>912)
                     /locus_tag="ACHKW5_005652"
     CDS             complement(114..>912)
                     /locus_tag="ACHKW5_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7514772.1"
                     /translation="QRESEEQRLRDVYGDNPDALAKATSALKNTWSAEEQLRGSWMAG
                     LKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQSWRGFTRSVLSMLTE
                     IFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQAAAANFHFATGGFTGTGGKYEPA
                     GIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGISFNQNNH
                     VVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQMRDGGVFSGGRR"
BASE COUNT          183 a          337 c          207 g          185 t
ORIGIN      
        1 agcattcagc cccgccgcca tacgctgcga atacccgtca ccaaaacgca cttcacgcac
       61 cgatggctgc gagttcacct ccatatccgg cttcactttc cagcgaaatg ttttcatcgc
      121 ctgcctccgg aaaagacgcc gccatcacgc atctgcgcct gaatctcatc ctgcgccccc
      181 ttgcgggcca tgtcatacac cgccttcatc agctgcggcc ccgcctgtcc gttggtgccg
      241 tcgttctgaa tcaccacgtg attgttctga ttaaaactaa tgccttccgc ccgccgcatc
      301 tgcgccggac ttccggcacc gcccacataa ccaccttccg catacccgcg catcagacga
      361 tacaggttgc cgacgccaat ccggctggtt gcctccttcg tgaagacaaa ctccccgcgg
      421 tggacaatac cggcaggttc gtatttgcca ccggttcccg taaatcctcc ggtcgcgaaa
      481 tggaagttcg ccgccgcagc ctgaatggcc gtccccgtgg aggcagacgc cccaccaccg
      541 aaagcaccac ccatggcgct gccaatactc ccgacaatcc ccaccatggc ctgcttcaga
      601 aaaatctctg tcagcatgga gagcacagaa cgggtgaaac cacgccagct ctgttcgctg
      661 ccggtcagca tcgctgccat attctgtgca ataccgtcaa aggtctgcgt ggccacgctt
      721 ttaacctgcg aaaaactgtc cgtcgcactt tctgcccact cgccccagcc ggacttcaga
      781 ccggccatcc agcttccacg aagctgctcc tccgcagacc aggtgttctt cagtgcagat
      841 gtggccttcg ccagcgcatc cggattatca ccgtacacgt cacgaaggcg ctgctcttcc
      901 gactcccgct gc
//
LOCUS       DAZFYC010000404          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFYC010000404 DAZFYC010000000
VERSION     DAZFYC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.411"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..843
                     /locus_tag="ACHKW5_005653"
     CDS             <1..843
                     /locus_tag="ACHKW5_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514773.1"
                     /translation="STLLTMSPLLMLNGAVKTGSIVRTIGRGVSYADIPLALAILGDS
                     WYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLSLMSSLSGAIMRSLEKGTPPQ
                     MMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNPDILNTQAIALCVDLQHTKNI
                     TMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQGERAILNTSQSETCKHKIDE
                     IYEKRFSETELNTLPNEMKNFLIFLNKTHEKDISRLSMGNEVNEKIIAVIVKTLAYRE
                     SMLC"
BASE COUNT          345 a          172 c          149 g          240 t
ORIGIN      
        1 agtacattac taaccatgtc accactgtta atgcttaatg gagcagttaa aacaggtagc
       61 atagtaagaa ctataggtag gggagtgagt tatgctgata taccattggc cttagctata
      121 ttaggtgact catggtataa agcttataaa catggttctt ctgataaccc aaattctgct
      181 cagagattta tatcgcaaga agcggccttt aaaaccaccc agcgggtatt aacacaagga
      241 ttaagtctga tgtcctcttt atcgggagca atcatgcgct ctcttgagaa aggcacacca
      301 ccacaaatga tgtctttatt catcgtaaac atactaaatc tattatttca tcaaaatcca
      361 tatgaagggg catcagcaag tgctaatgct ttgaaaagat caacttactc ccataatccg
      421 gacatactaa atactcaggc aatagctctt tgtgttgacc tccagcatac aaaaaatata
      481 accatgccac ttttcaaaac aaaagacagg atatcttacg cattcaatgg acaaagaaca
      541 tccccagaag accaaaaaca aatactgaaa gaagttataa actcctgtac ccaaggagaa
      601 agagccattt taaatacatc acaatcagaa acatgcaaac ataaaatcga tgagatttat
      661 gaaaaaagat tctcagaaac agaactaaat acattaccaa acgaaatgaa aaatttcttg
      721 atatttttga acaaaactca tgagaaagat atttcgcgtt taagcatggg taacgaagtt
      781 aatgaaaaaa taattgcagt tatagtcaag acgttagcat atagagagtc tatgttgtgt
      841 tagactttaa ctctacattg atataacatt agccaaatat tcaacagtgt atgcgaccaa
      901 acacca
//
LOCUS       DAZFYC010000405          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFYC010000405 DAZFYC010000000
VERSION     DAZFYC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.412"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..>906
                     /locus_tag="ACHKW5_005654"
     CDS             32..>906
                     /locus_tag="ACHKW5_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO7514774.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHKQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNSLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNTLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEIIEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTF"
BASE COUNT          364 a          158 c          160 g          224 t
ORIGIN      
        1 cataaataat tttttaagcg gaaatgtaat tatgagactg ccagagaaag ttctttttcc
       61 tcctgtcact agtggcctgt cagggcagga aaaacaaaaa aaaccgaaga gcattaccgg
      121 atttcaggaa aattatcaac gcaatatcag gccaatcaaa acagcatcag aagcccgact
      181 acgcttcttt gataaaatgg tttcgaaaga aaactctctg gaagatgttg tttctttagg
      241 tgaaatgatt cagaaggaaa tttatgggca taaacaaaga acattttcac cagttcatca
      301 tacaggtaac tggaaatcat cattgttaca caacgcgctc cttggtctgg caaatgttta
      361 taatggctta cgggaaacag aataccctaa cactttcaac agagatggta taaaaagtac
      421 taactctttt agagataact cattgacaaa aacaagaact cccagagata attttgagga
      481 aggaataaaa catcctgaac atgcaacaat accatatgac aacgacaatg aaagtaatac
      541 attgctaaaa gcaggaaaga tagctggtaa caataacgag ctgttgatgg aaataaaaaa
      601 ggaatcccaa agcgaccatc aaatccccct gtcagataag ttcctgaaaa ggaaaaaacg
      661 atctcctgta gctgaagata aagttcaaaa ctcgttaaca ccagaaaatt ttgttcagaa
      721 aatttcactt agtgatgagc ttaaaacaaa atatgcaaat gaaattatag agataaaaag
      781 aataatggga gaatacaatc ttttaccgga taaaaacagt cgtaatggtc taaaacttct
      841 acagaagcaa gctgatttac taaaaataat catggaggat acctctgtta cagaaaatac
      901 ctttaa
//
LOCUS       DAZFYC010000406          871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFYC010000406 DAZFYC010000000
VERSION     DAZFYC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.413"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..684
                     /locus_tag="ACHKW5_005655"
     CDS             <1..684
                     /locus_tag="ACHKW5_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7514775.1"
                     /translation="CNEAKLELVRMNIIKQQGGMFGPNKNISEWCIPQNEGGSPKMRD
                     IPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSSENSGESSDQPENDL
                     SVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDG
                     RNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTN
                     TDWIYGVDL"
     gene            681..>871
                     /locus_tag="ACHKW5_005656"
     CDS             681..>871
                     /locus_tag="ACHKW5_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HFO7514776.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEV"
BASE COUNT          278 a          218 c          224 g          151 t
ORIGIN      
        1 tgcaatgaag ccaagttaga actcgtcaga atgaatatta tcaagcagca aggcggcatg
       61 tttggaccaa ataaaaacat ctcagaatgg tgcatccctc aaaacgaggg aggttcccct
      121 aaaatgaggg acatccctca aaacgaggga aaatccccta aaacgaggga taaaacatcc
      181 ctcaaattag gggattgcta tccctcaaaa cagggggaca caaaagacac tattacaaaa
      241 gaaaaaagaa aagattattc gtccgagaat tctggcgaat cctctgacca gccagaaaac
      301 gacctttctg tggtgaaacc ggatgctgca attcagagcg gcagcaagtg ggggacagca
      361 gaagacctga ccgccgcaga gtggatgttt gacatggtga agaccatcgc gccatcagcc
      421 agaaaaccga attttgcagg gtgggctaac gatatccgcc tgatgcgtga acgtgacgga
      481 cgtaaccacc gcgacatgtg cgtgctgttc cgctgggcat gccaggacaa cttctggtcc
      541 ggtaacgtgc tgagcccggc caaactccgc gataagtgga cccaactcga aatcaaccgt
      601 aacaagcaac aggcaggcgt gacagccagc aaaccaaaac tcgacctgac aaacacagac
      661 tggatttacg gggtggatct atgaaaaaca tcgccgcaca gatggttaac tttgaccgtg
      721 agcagatgcg tcggatcgcc aacaacatgc cggaacagta cgacgaaaag ccgcaggtac
      781 agcaggtagc gcagatcatc aacggtgtgt tcagccagtt actggcaact ttcccggcga
      841 gcctggctaa ccgtgaccag aacgaagtga a
//
LOCUS       DAZFYC010000407          863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFYC010000407 DAZFYC010000000
VERSION     DAZFYC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.414"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..847)
                     /locus_tag="ACHKW5_005657"
     CDS             complement(<1..847)
                     /locus_tag="ACHKW5_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514777.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENESAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYP"
BASE COUNT          245 a          129 c          174 g          315 t
ORIGIN      
        1 taggatattg atagaatatt gttggtgcat gaaataaagt tgctgatagt aaatcaccaa
       61 gtatgcgtac ctgggttata tctagtgata atacattact taagaatgct tctgtattta
      121 ttgttggaga aggtgatgta tataaagatg gttgttgaga ctgtgaaggt gatttgttat
      181 agtcgccatt aaaaaataat gcctgtatga gcaagtgaat gctgattccg cctccggctc
      241 tgaacgcata tggaagtatc tgttcgattt tattgtgtat ggccatatag gctttataaa
      301 aacatcctga ctgattatat acctgatgat attgatcgtt cagaactctc ataagatgga
      361 gaacagtttc tgcactctca ttctcttttg gtataatttc atgaatgatg ctgtccagtt
      421 gatatttttg cttgcatgtt aggatttttg taattctttc atcgatttca atgcatattg
      481 aattattttt atcggataat aacaaatcat tttcctgtgt atttgtttgt atctcttgtg
      541 ggatattaat taccacatcc gtacatgttc tatccacctc atttgtcttt atgttgtcaa
      601 gaaaatcatt taacgtcagt tgagaaccca attcattaat gtagtccaat gatgacgtag
      661 aagagctatt atcaagaaaa tcgttcagag tgagctcgga gtcgtttctt gttatcggat
      721 atacatttgt ggaagcggct aatttaatac tccggttgct ggaggtagaa gctgttggtt
      781 cagaggttga cgaacactgc atgtcaatgc atacataacc tttatttgaa gttgaatttg
      841 gaatcaagtt tcctcctgaa tta
//
LOCUS       DAZFYC010000408          862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFYC010000408 DAZFYC010000000
VERSION     DAZFYC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.415"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..103
                     /locus_tag="ACHKW5_005658"
                     /pseudo
     CDS             1..103
                     /locus_tag="ACHKW5_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            103..264
                     /locus_tag="ACHKW5_005659"
                     /pseudo
     CDS             103..264
                     /locus_tag="ACHKW5_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(314..667)
                     /gene="cmi"
                     /locus_tag="ACHKW5_005660"
     CDS             complement(314..667)
                     /gene="cmi"
                     /locus_tag="ACHKW5_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO7514778.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYNCEEGYVEYAND"
BASE COUNT          269 a          167 c          143 g          283 t
ORIGIN      
        1 acagttttta tctttaaaaa gagttaatgg aatggatagt attaaattac ttgataaagt
       61 tattatctat caacaacaga atgagcaatt atatccagca caggcgttgt tcgttcatat
      121 gatgataaat acgattttaa cgccagcact caccgtggca tcatcggaga gtcgctcaca
      181 aggctcgggg cgatgttttc tggtaaagag taccagatac tgcttcctgg tgaaattcac
      241 attaaagaaa gtggtaagcg ataatataag ggccgggatt ccggcctttt tatgcagcct
      301 ttataacagt gtattagtca ttcgcatatt caacatatcc ctcctcacaa ttgtatatgc
      361 cataatacat tctcttaccc tccgggcctt cagctttaaa tgggacaaga agtgtttttt
      421 catcttcaac atctgttaaa cttctctcaa tcttttccca tgtaattttc gggatttctg
      481 taccaagaag ttgaacatcg ttgtcccagc gtttcattct ttgcaggtaa tcatttttta
      541 caaacgttct ggcctgttca tcgctgacct gatagcatgc cggaccttta tcttcagacc
      601 agaaaaaaac agcagcaaga gcaataatcg ttaaaataaa aataaatttc atgctaatta
      661 ctttcataaa atctccacaa cttctgtcgt tcattcgata taaaaaacat tacgaatata
      721 tccgtacaat gtgagcatac tgtgaagtgg ctttaacaat tatgaaataa tacaatagat
      781 cacatattgc agtggtctct tttttataga agaaatcaca attcataatt gtgacttatt
      841 atgcatgttt gtttttcagc at
//
LOCUS       DAZFYC010000409          860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFYC010000409 DAZFYC010000000
VERSION     DAZFYC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.416"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>860
                     /locus_tag="ACHKW5_005661"
     CDS             <1..>860
                     /locus_tag="ACHKW5_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7514779.1"
                     /translation="YGGPVAITPYTLMQAVAIEGEPQVGPSSEPDWYYVIVLAGQSNA
                     MAYGEGLPLPDSYDAPDPRIKQLARRSTVTPGGAACRYNDIIPADHCLHDVQDMSTLN
                     HPRADLSKGQYGCVGQGLHIAKKLLPYIPNNAGILLVPCCRGGSAFTQGAEGTFSEST
                     GASQDSARWGVGKPLYQDLISRTKAALQKNPKNVLLAVCWMQGEFDMSAATHAQQPAL
                     FTAMLTQFRADLSVFNAQCHGGSAADVPWVCGDTTYYWKNTYATQYDTVYGGYKNRES
                     EGVYFVPFMT"
BASE COUNT          188 a          222 c          262 g          188 t
ORIGIN      
        1 tacggcggac cggttgccat tacgccgtac acactgatgc aggcggtggc tattgaagga
       61 gagccacagg tcggcccttc atctgagccg gattggtact acgtcatcgt actggccggg
      121 cagtccaatg ccatggctta cggtgaaggg cttccgctgc cggattcata cgatgctccg
      181 gatccgcgca ttaaacagct ggcgcgccgc agtacagtga cgccgggcgg ggctgcctgc
      241 agatataacg atattattcc ggctgaccac tgtctgcatg atgtgcagga tatgagtacg
      301 ctgaatcatc cgagggctga cctgagcaaa gggcagtacg gctgtgtcgg tcagggttta
      361 catattgcca aaaaactgct cccgtatatc ccgaataacg cggggatcct gctggtacca
      421 tgctgtcgtg gtggttcggc atttacccag ggcgcggagg ggacattcag cgagtccacg
      481 ggggccagtc aggattcggc acgctggggg gtgggcaagc cgttatatca ggatctgatt
      541 tcccgcacaa aagcggcatt gcagaaaaat cccaaaaacg ttctgctggc cgtctgctgg
      601 atgcagggtg agtttgacat gagcgccgcc acccacgcac agcaacctgc gctgtttaca
      661 gccatgctga cacagtttcg tgctgacctc tccgtgttta acgcgcagtg ccatggtggc
      721 agtgctgcag atgtgccgtg ggtttgtggt gacacgacgt attactggaa aaatacatac
      781 gctacccagt acgacaccgt gtacggcggg tataaaaaca gggagagtga gggcgtttat
      841 tttgtgccct tcatgacaga
//
LOCUS       DAZFYC010000410          858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFYC010000410 DAZFYC010000000
VERSION     DAZFYC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.417"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..251)
                     /locus_tag="ACHKW5_005662"
     CDS             complement(<1..251)
                     /locus_tag="ACHKW5_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7514780.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPG"
     gene            complement(316..>858)
                     /locus_tag="ACHKW5_005663"
     CDS             complement(316..>858)
                     /locus_tag="ACHKW5_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7514781.1"
                     /translation="APAWASEQQATLSAGYLHARTNAPGSDNLNGINVKYRYEFTDTL
                     GMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAY
                     SRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSDTSLAWGAGVQFNPTESVAIDIAYE
                     GSGSGDWRTDGFIVGVGYKF"
BASE COUNT          190 a          272 c          205 g          191 t
ORIGIN      
        1 cccggttgag aatcttcata cacggtgatg gtcccggcat gtgacggcgg gaagccctcc
       61 accaacagaa tgacgctgta ctgaccgtac tcaacgtcca tgctgtaacg accggcttca
      121 tccggatttt cagaggccac cgtattcacc accaccgtgc tgctggtccg tctggctttc
      181 agttgaatgg tgcagttctc taccggtttt cctgtgccgt ctttcagtac acctgaaatc
      241 tttactgcca tattcccccc acaaaaaagc ccgcctgaac cggcgggctg tcataacact
      301 gtgttacctg gctaatcaga atttataacc gacacccacg atgaaaccgt cagtgcgcca
      361 gtcgccactg ccggagcctt cataagcaat atcaatggcc acggattcgg tcgggttaaa
      421 ctgcacgcca gctccccacg ccagagaggt gtcgctgtgg cgaccgtcat cacttccggt
      481 cagcacatcg tgcgttttcc ccttgttgtc agttacgcga agataatccc cggagaaagt
      541 cgacacacgg ctgtaagcca cacccgccat cgcatacgcg ctgaaccatt cattcacgcg
      601 cacagacggc cccgccatta cgctgaacca gcggttacga acggaatctt catgccagcg
      661 ggtatcgctg taacgggtaa gctggcgatt cctgtctcct gcatagctga atgacgtcac
      721 catccccagc gtatccgtaa actcataacg gtatttcacg ttaatcccgt taagattatc
      781 gctaccggga gcgttcgtcc gggcatgaag ataccccgcg ctcagcgtgg cctgctgctc
      841 agacgcccat gcaggcgc
//
LOCUS       DAZFYC010000411          851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFYC010000411 DAZFYC010000000
VERSION     DAZFYC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.418"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..849
                     /locus_tag="ACHKW5_005664"
     CDS             <1..849
                     /locus_tag="ACHKW5_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7514782.1"
                     /translation="GEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLAD
                     PAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANN
                     ITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKLD
                     TRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNT
                     QARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEF
                     VSVQLA"
BASE COUNT          194 a          235 c          259 g          163 t
ORIGIN      
        1 ggtgaggtta tccgttcccg tggcggctcc acctctgaat ttacgccggg atatgtcaag
       61 ccgaagcatg aagtgaatcc gcagatgacc ctgcgtcgcc tgccggatga agatccgcag
      121 aatctggcgg acccggctta ccgccgccgt cgcatcatca tgcagaacat gcgtgacgaa
      181 gagctggcca ttgctcaggt cgaagagatg caggcagttt ctgccgtgct caagggcaaa
      241 tacaccatga ccggtgaagc cttcgatccg gttgaggtgg atatgggccg cagtgcggcg
      301 aacaacatca cgcagtccgg cggcacggag tggagcaagc gtgacaagtc cacgtatgac
      361 ccgaccgacg atatcgaagc ctacgcgctg aacgccagcg gcgtggtgaa tatcatcgtg
      421 tttgacccga aaggctgggc gctgttccgt tccttcaaag ccgtcaggga gaagctggat
      481 acccgtcgcg gctctcattc cgaactggag acagcggtaa aagacctggg caaagcggtg
      541 tcttataagg gaatgtatgg cgatgtggcc atcgtcgtgt attccggaca gtacgtggaa
      601 aacggcgtca aaaagaactt cctgccggac aacacgatgg tgctggggaa cactcaggca
      661 cgcggtctgc gcacctatgg ctgcattcag gatgcggacg cacagcgcga aggcattaac
      721 gcctctgccc gttacccgaa aaactgggtg accaccggcg atccggcgcg tgagttcacc
      781 atgattcagt cagcaccgct gatgctgctg gctgaccctg atgagttcgt gtccgtacaa
      841 ctggcgtaat c
//
LOCUS       DAZFYC010000412          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFYC010000412 DAZFYC010000000
VERSION     DAZFYC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.419"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..>846
                     /locus_tag="ACHKW5_005665"
     CDS             47..>846
                     /locus_tag="ACHKW5_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7514783.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDTNIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREV"
BASE COUNT          277 a          168 c          165 g          236 t
ORIGIN      
        1 caaactatgt gatctccatt tcgattgatt tagtgtttat tgacgtatgt actggattat
       61 taacgataat atcgagttct ggcctgagca ccgaaaatta atatcggtac ataacgccga
      121 tcttaacgtc gttctgacaa cgccagccag tcgatgttta tcacttctac ttgaagcttt
      181 tcctgatgtg gttgcacaac aagatttttt caccagagtc tgggaagaag aaggtatgcg
      241 tgtgcctact aacacgttat atcagaacat atccattatc agacgcggat ttcgcgctgt
      301 tggtgatact acccactcgc taattgcaac cgtgccgaga agaggattca agatccataa
      361 tgacactaac atacaaaatc atgtaataaa ctcgtcaaca gacgcacata cacacaatgc
      421 cccacctgcc ataaaagtta atgcgggata caaagagagc attggtggcg caaagaattt
      481 taataacaaa atcctcaaac atataaaaag tcatctaatt atgttgagcg catttgtcat
      541 aggcgcatat tctgcatatt ggctatggaa taataatcaa ccgaagccat ttttcaagga
      601 ttacaagact gttgcggaaa ttaatggctg ccattttaat gtaacagaag atacaattga
      661 cgggttgaaa gagttcgata aatataaaac acggatactg gattcgggga ttaactgtaa
      721 aaaacatccg tggttatact tccctcttgc aaaatcctca ccggggatga ttgttatggc
      781 atgcaataaa aattataacc aacatgaagt ggcagactgt ttaaccctgt cttaccgtga
      841 ggttaa
//
LOCUS       DAZFYC010000413          840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFYC010000413 DAZFYC010000000
VERSION     DAZFYC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.420"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            219..809
                     /locus_tag="ACHKW5_005666"
     CDS             219..809
                     /locus_tag="ACHKW5_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7514784.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT          280 a          165 c          174 g          221 t
ORIGIN      
        1 cactattctt cttccttgag tccgcccgga actcgaaaaa caaaccgagt taaggccatt
       61 tttcacaaaa tcgattttgg gtctcaccaa aattacgggg ttgcatacgc attcgtttat
      121 tttcgaacgt gtacatacaa atatgtacaa aaataatcaa aattattttc tgagatgcat
      181 tatgatatgt acaccaattt cgtatagaag atctcactat gtctcgaatt tatgcttact
      241 gtcgggtatc aactctggaa cagacaacca aaaatcaacg tcgggaaatc gaaagtgcag
      301 gttttaacat caaatctcag caaataattg aagaacaaat tagcggctca gcagcaacca
      361 gtgagcgtcc tggttttaac cagttgcttg atcgactgaa aagaggtgat gcattgattg
      421 tcacaaaact ggatcgcctc ggttgtaatc caatggatat cagtaaaacg gtggaacaac
      481 tgaccaaagc aggtatcaaa gtgcattgct tagcattggg gggcgttgac ctgaccagtc
      541 cacctggaaa aatgatgatg caagtaattt cagcagtcgc tgaatttgag cgagaccttt
      601 tacttgagcg tactcattca gggatattaa gagcacgggg cgcaggaaag cgatttggtc
      661 gcccacctgt gttaaatgat gaacaaatac agagggtact agagcaaatt aagtcaggta
      721 taaatataag tgacattgcc caagaattca aaacctcgcg gcaaaccatt ttaagagcta
      781 aagcaaaaaa tcagacacct gatatataaa aataatctcg gtgtgagatt ccttacgttt
//
LOCUS       DAZFYC010000414          839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFYC010000414 DAZFYC010000000
VERSION     DAZFYC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.421"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..824
                     /locus_tag="ACHKW5_005667"
     CDS             15..824
                     /locus_tag="ACHKW5_005667"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO7514785.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT          290 a          113 c          166 g          270 t
ORIGIN      
        1 tagaagtatt aacaatgaaa gttcaattgt acaaattcac ggaagataaa aataaaaccc
       61 ttaccttcag atggacgaaa aaacactttg agttttgtat ggataataaa atctttttaa
      121 atcataaggg gaagaagtcg tataaagaaa ggaatttatt tcttttctca aaaggtgaca
      181 agataacaat tgaagataat gttattgctg aagaatattc aacaatgcct gtgaaaaatt
      241 tctccagtgt cggcgctttt tcatttccga cctgccattt ttcagggaat atacgtatag
      301 gtcgattttg tagtattgca tcaaatgtaa aaataatggg tggaaatcat cctttgaatc
      361 gttttactac tcacatgatg acttataacg gtgaatttga taagtttgct atgagtgaat
      421 ttgaacgtag ctggacattg aaaccattca ttaccaaacc tgaaaatccg attataggaa
      481 atgatgtctg gataggaaat gatgttgtat tgaaaggtgg catcgcaata ggtgatggtg
      541 ctgttattgc tgccaattcc gttgttacca aagatgtgcc cccatatgcc atagttgcag
      601 gagttcctgc aaaaataatc agatttaggt ttgattcaaa tgttattgat gaactgttga
      661 gaataaaatg gtggaattat aactactcag atcttccgga taataacaag tgtgatgata
      721 taaattattt tgttgaagag atgaatcgat tgatatcaga tggaaatatt caagagaggg
      781 actataaaaa attcaatctt tctgaggtat tcagaggcct ataattatct gttttgcat
//
LOCUS       DAZFYC010000415          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFYC010000415 DAZFYC010000000
VERSION     DAZFYC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.422"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..761
                     /locus_tag="ACHKW5_005668"
     CDS             <1..761
                     /locus_tag="ACHKW5_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514786.1"
                     /translation="AVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELL
                     SRIGSYKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKF
                     SHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRV
                     MGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEE
                     RRLMLQWWADFLDANRERFISPFEYAKINNPLKQ"
BASE COUNT          232 a          195 c          198 g          213 t
ORIGIN      
        1 ctgccgtaat gcgttatgcc gtacagtctg gcatcatcag atataatcct gctctggata
       61 tggctggcgc attgactacg gtaaaacgcc agcatcgccc cgctcttgat ctttctcgcc
      121 tgcctgaact tttgtcgcgt attggcagtt acaaggggca acctgtcacc cagcttgccg
      181 ttatgctgaa tttactggtt tttattcgtt ccagtgaact cagatacgcc cgctggtctg
      241 aaattgatat tgacaatgcc atgtggacta ttccagccga acgcgaacct ctgccaggcg
      301 taaaattctc acaccgggga tccaagatgc gaacaccaca tcttgtgcca ctcagcaaac
      361 aggttgtagc catactggca gaacttcaga catgggctgg tgaaaatggt ctgatcttta
      421 cgggagcaca tgacccacgt aaaccaatca gtgaaaacac cgtaaataaa gccctgaggg
      481 ttatggggta tgacacaacc caggatgtct gtggtcatgg attccgggcg atggcatgta
      541 gtgcgttaat agaatcaggt ttgtggtccc gcgatgcagt tgaacgtcag atgagccatc
      601 aggaacgtaa cggtgtacgt gctgcttaca ttcataaagc agaacatctg gaagaacgtc
      661 gactgatgct acagtggtgg gccgattttc tggatgcaaa cagagaaaga tttatcagtc
      721 catttgaata tgcaaagatt aataatccat taaaacagta atcatcccgg gcaaatgccc
      781 gggaacctac tccaggatta ttttctttgt taaaaaaaca gacaaaacgg tattaact
//
LOCUS       DAZFYC010000416          836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFYC010000416 DAZFYC010000000
VERSION     DAZFYC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.423"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..697
                     /locus_tag="ACHKW5_005669"
     CDS             <1..697
                     /locus_tag="ACHKW5_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7514787.1"
                     /translation="PAESCGFVISTPEGERYLPCVNISAEPEAYFRIAPEDWLRAEMS
                     GEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSQGDIHKFRCVPHLTGRRFEHGV
                     TDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDIL
                     LCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFT
                     GIYNDLAAASACM"
     gene            595..>836
                     /locus_tag="ACHKW5_005670"
     CDS             595..>836
                     /locus_tag="ACHKW5_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514788.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SLQVPGFRRQMNEGWYQIRIAGEDTAPEAVYARLHEP"
BASE COUNT          161 a          228 c          269 g          178 t
ORIGIN      
        1 gcctgcggag tcgtgcggct tcgtgataag cacgccggag ggggagcggt atctgccgtg
       61 tgtgaatatt tccgcagagc cggaggcgta ttttcgtatc gcaccggaag actggctgcg
      121 ggcagagatg tcgggggaga ttgtggcgct ggttcacagt catcccggtg gtctgccctg
      181 gctgagcgag gctgaccggc ggctgcagat aaaaagcgca ctgccctggt ggctggtcag
      241 ccagggtgac attcacaaat tccgctgcgt tccgcacctg accggacggc gctttgaaca
      301 cggggtgacg gactgttaca cgctgttccg ggatgcatac catctggcgg gaattgatat
      361 gccggatttt gagcgtgagg atgactggtg gcgcaacggt cagaaccttt acctggacaa
      421 tatggcggtc accggctttt accgggtgcc cctgtcctct gcacaggcgg gcgatattct
      481 gctgtgctgc tttggtgctt cggtaccgaa ccatgccgcc atttactgtg gcaacggtga
      541 gctgcttcac catctgcctg aacaactgag taaacgggag aggtattccg aaaaatggca
      601 acgacgaacg cattctgtct ggcgtcaccg ccactggcac gcatctgcct tcacggggat
      661 ttacaacgat ttggccgccg cctcagcctg tatgtgaaca cggcagcgga agccatccgg
      721 gcgctgtcgt tacaggtgcc cggattccgc cgtcagatga acgaaggctg gtaccagata
      781 cgtattgccg gtgaggacac ggcaccggag gcggtgtacg cccgtcttca cgaacc
//
LOCUS       DAZFYC010000417          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFYC010000417 DAZFYC010000000
VERSION     DAZFYC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.424"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>819
                     /locus_tag="ACHKW5_005671"
     CDS             <1..>819
                     /locus_tag="ACHKW5_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7514789.1"
                     /translation="GGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNN
                     PAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYL
                     SQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRY
                     SFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAH
                     RAGLWVIKTGLLETQMVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDA"
BASE COUNT          176 a          228 c          267 g          148 t
ORIGIN      
        1 ggcggtcagc agatgacggt gaactaccat atccgcggtc gcatcatcca ggtgccgtca
       61 aactacgacc cggaaaaacg cacttacagc ggtatctggg acggcagcct gaaaccggca
      121 tacagcaaca acccggcctg gtgcctgtgg gacatgctga ctcacccgcg ctacggcatg
      181 ggaaaacgtc tgggggcggc agacgtggac aaatgggcgc tgtatgccat tgcgcagtac
      241 tgcgaccaga cggtcccgga tggcttcggg ggcacagagc cgcggatgac ctttaatgca
      301 tacctgtcac aacagcgtaa ggcgtgggac gttctcagtg atttctgctc ggcgatgcgc
      361 tgtatgccgg tatggaacgg ccagacgctg acgttcgttc aggaccgccc gtcggatgtg
      421 gtgtggccgt acaccaacag cgatgtggtg gtggatgata acggcgtggg gttccgctac
      481 agcttcagtg ccctgaagga ccggcacacg gcggtggagg tgaattacac cgacccgcag
      541 aacggctggc agacctccac ggaactggtg gaagacccgg aagccatact gcgctacggg
      601 cgcaacctgc tgaagatgga tgcgttcggt tgcaccagtc gcggtcaggc ccaccgtgcc
      661 gggctgtggg tgataaagac cggactgctg gaaacgcaga tggtggattt cacgctcggg
      721 tcacaggggc tgcgtcacac acccggtgac atcattgaaa tctgtgataa cgactatgcc
      781 gggaccatga ccggcggacg tgtcctgtcc attgatgcc
//
LOCUS       DAZFYC010000418          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFYC010000418 DAZFYC010000000
VERSION     DAZFYC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.425"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..>814)
                     /locus_tag="ACHKW5_005672"
     CDS             complement(50..>814)
                     /locus_tag="ACHKW5_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7514790.1"
                     /translation="MFTGLSVDAVTGTEAAVFEGQSGIEAGLADELVNASDAISVMAT
                     ALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLATMLAGQQGMSVAQAR
                     AILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPEMTVEKARPILAASPQ
                     ADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAAGSGKAEPVSASTTA
                     LFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          139 a          273 c          211 g          191 t
ORIGIN      
        1 ttttcgttac catattgtaa aaacctcctg ttgatgatca gcactgactt catggcatgg
       61 ccatgagcat tttcacgtcc gcatcaccgt ctgctgacgt ctgtggcacg ccaccctgta
      121 ccgctgccgg tgaatggttc gccatgatgc gttcaaacag ggcggttgtg gatgcagaga
      181 ccggttctgc cttacctgat cccgcagcca gcacagcccg ggcgctctcc acagtcattc
      241 ccgggcaggc agccagttgt tcagcctgcg cctcagcccc ttttgcctca tccagtgcca
      301 tgatctgatc acggagtgag ggtccggcat ccgcctgcgg tgaagcagcc aggatcgggc
      361 gggctttttc caccgtcatc tccggcatcg ccgccagcgt tgccgccagt tgttcacgac
      421 cgttcgcttc ttcacacgcc ataatgcgat cggcttcact ctgcgtggat gccaccggct
      481 gctgcggtgc cgccgcggcc agaatcgccc gggcctgtgc aacgctcatg ccctgttgtc
      541 ctgccagcat cgtggcaagc tgttcacgtc ctttcgcttc ctggcatgtc aggatcccca
      601 tcactcgctg gttctcctgc gcggcggctt ccgttgcagt taattgcggc atagtgcctc
      661 ctctgacatt actgttcagc gccgtggcca tcacactgat ggcatccgac gcattgacta
      721 attcatccgc cagcccggcc tcaatgccgg actgaccttc aaaaacggcg gcctctgttc
      781 ccgtgacggc atcaacagac agaccggtaa acat
//
LOCUS       DAZFYC010000419          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFYC010000419 DAZFYC010000000
VERSION     DAZFYC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.426"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..>814)
                     /locus_tag="ACHKW5_005673"
     CDS             complement(50..>814)
                     /locus_tag="ACHKW5_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7514791.1"
                     /translation="MFTGLSVDAVTGTEAAVFEGQSGIDAGLADELVNASDAISVMAT
                     ALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLATMLAGQQGMSVEQAR
                     AILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPEMTVEKARPILAASPQ
                     ADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAAGSGKAEPVSASTTA
                     LFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          139 a          273 c          212 g          190 t
ORIGIN      
        1 ttttcgttac catattgtaa aaacctcctg ttgatggtca gcactgactt catggcatgg
       61 ccatgagcat tttcacgtcc gcatcaccgt ctgctgacgt ctgtggcacg ccaccctgta
      121 ccgcagccgg tgaatggttc gccatgatgc gttcaaacag ggcggttgtg gatgcagaga
      181 ccggttctgc cttacctgat cccgcagcca gcacagcccg ggcgctctcc acagtcattc
      241 ccgggcaggc agccagctgt tcagcctgcg cctcagcccc ttttgcctca tccagtgcca
      301 tgatctgatc acggagtgag ggtccggcat ccgcctgcgg tgaagcagcc aggatcgggc
      361 gggctttttc caccgtcatc tccggcatcg ccgccagcgt tgccgccagt tgttcacgac
      421 cgttcgcttc ttcacacgcc ataatgcgat cggcttcact ctgcgtggat gccaccggct
      481 gctgcggtgc cgccgcggcc agaatcgccc gggcctgttc aacgctcatg ccctgttgtc
      541 ctgccagcat cgtggcaagc tgttcacgtc ctttcgcttc ctggcatgtc aggatcccca
      601 tcactcgctg gttctcctgc gcggcggctt ccgttgcagt taattgcggc atagtgcctc
      661 ctctgacatt actgttcagc gccgtggcca tcacgctgat ggcatccgac gcattgacta
      721 attcatccgc cagcccggca tcaatgccgg actgaccttc aaaaacggcg gcctctgttc
      781 ccgtgacggc atcaacagac agaccggtaa acat
//
LOCUS       DAZFYC010000420          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFYC010000420 DAZFYC010000000
VERSION     DAZFYC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.427"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..338)
                     /locus_tag="ACHKW5_005674"
     CDS             complement(<1..338)
                     /locus_tag="ACHKW5_005674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514792.1"
                     /translation="MREHNYAIGGYFSLELQALKNNTANEKVYLQSARACFQLLLESI
                     EVSRVWLPYYICDVVVDTINEIGIEILYYSISKDFIPQTFPVLEENDVFVYVNYFGVC
                     DEQTKLILQK"
     gene            complement(342..>803)
                     /locus_tag="ACHKW5_005675"
     CDS             complement(342..>803)
                     /locus_tag="ACHKW5_005675"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HFO7514793.1"
                     /translation="IIITIEQAYKKSVFFDEVFPVIYGVLSKEYDFISDLAITSLLSI
                     KNKLDIGAEVVLTSTNYGNNNLTSQDRVIDINVKEHASTYINSEGGRLLYDKKTFKLN
                     GVNLKFIHPEILPYKQLCNGEFVPSLSIIDVVMNNGWDTTKQLVNSFELKD"
BASE COUNT          274 a          148 c          102 g          279 t
ORIGIN      
        1 tatttttgca aaattaactt tgtttgctca tcacacaccc caaaataatt aacatagaca
       61 aaaacatcat tttcttctaa aactggaaac gtctgcggaa taaaatcttt agaaatacta
      121 taatacaaga tttcaatccc aatttcattt attgtatcaa cgacaacatc acatatataa
      181 tatggtaacc atactctact aacttcaatg ctttctagca gtaattgaaa acatgccctc
      241 gctgattgta aatatacttt ttcatttgca gtgttatttt taagcgcctg caattctagt
      301 gaaaaatatc ctccgatcgc ataattatgc tcacgcattg ttcaatcctt cagttcaaag
      361 ctatttacta actgctttgt agtatcccaa ccattattca ttactacatc tataattgat
      421 aaggatggta caaactcacc attacaaagt tgtttgtaag gtaaaatttc tggatgtata
      481 aatttcaaat ttacgccatt tagtttgaat gtctttttat cataaagtaa tctcccgcct
      541 tcagaattaa tataagtgga cgcatgctct tttacattaa tatcaatgac gcgatcttgc
      601 gaagttaaat tattgttacc atagttggtc gaagtcaaaa caacttctgc tccaatatca
      661 agtttgtttt ttattgacaa taatgacgtt attgccagat cggatatgaa atcatactct
      721 ttagataaca caccataaat gactggaaag acctcatcaa aaaaaacgga ttttttatag
      781 gcttgttcaa ttgtaataat gat
//
LOCUS       DAZFYC010000421          801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFYC010000421 DAZFYC010000000
VERSION     DAZFYC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.428"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..194
                     /gene="dicB"
                     /locus_tag="ACHKW5_005676"
     CDS             6..194
                     /gene="dicB"
                     /locus_tag="ACHKW5_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7514794.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            191..379
                     /locus_tag="ACHKW5_005677"
     CDS             191..379
                     /locus_tag="ACHKW5_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7514795.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            475..>801
                     /locus_tag="ACHKW5_005678"
     CDS             475..>801
                     /locus_tag="ACHKW5_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFO7514796.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKL"
BASE COUNT          232 a          183 c          180 g          206 t
ORIGIN      
        1 cgataatgga aacgttatta ccaaacgtta atacgtctga aggttgtttt gatattggtg
       61 ttctgctcag taaccgggag tttactgaag atgccattaa tatgaggaaa tatgagcctt
      121 atctgctcaa tgataactcc atactttccc gaattgctct tcttgaactt ggtattatcg
      181 gagaacagca gtgacttcag catttgcact ggtgatgacc gtttttctta taacgggtga
      241 gccacaaaat gtgattaccg gaatttatga cagtaagtca tcctgcattc aggtaaggga
      301 cgaacaaaaa atccccggtg aatgcctccc gttaaaaaaa gtatcgctga acctgaataa
      361 cgaaataccg gctggataac ccgccagcca tattaacgcc ataccaacgg attaaaaatg
      421 ccagcaatgg cagggattcg ttcaccctga aatctgtaat gaggttaaaa caaaatgagt
      481 aaagtcttta tttgcgccgc cattccggac gaacaggcaa taaaggaaga aggtgccgtc
      541 gctgtagcca ctgccattga agccggtgat gaacgtcgcg cccgcgcaaa atttcactgg
      601 caattcctgg aacattatcc ggctgctcag gactgcgctt ataaatttct tgtctgcgag
      661 gataaacccg gtataccccg ccctgccctc gattcctggg atgctgaata tatgcaggaa
      721 aaccgctggg atgaggaatc cgcttccttt attccggtcg aaccagaatc cgatccgatg
      781 aacgtcaatt ttgacaagct g
//
LOCUS       DAZFYC010000422          783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFYC010000422 DAZFYC010000000
VERSION     DAZFYC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.429"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..65)
                     /locus_tag="ACHKW5_005679"
     CDS             complement(<1..65)
                     /locus_tag="ACHKW5_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514797.1"
                     /translation="MQDIHGESLIESVKSEQSPRVV"
     gene            complement(65..394)
                     /locus_tag="ACHKW5_005680"
     CDS             complement(65..394)
                     /locus_tag="ACHKW5_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514798.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(391..>783)
                     /locus_tag="ACHKW5_005681"
     CDS             complement(391..>783)
                     /locus_tag="ACHKW5_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7514799.1"
                     /translation="IQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIG
                     VGNLYRLMRGYAEGGYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKA
                     VYDMARKGAQDELRLQLRDGGMLSGSRR"
BASE COUNT          154 a          286 c          175 g          168 t
ORIGIN      
        1 accacccgcg gtgactgctc tgatttaacc gactcgatca ggctttcccc gtgaatatcc
       61 tgcatcagtt caccacctgc ttaaactccg cgctgaactc aacgcgcaac atcccgaccc
      121 gcgcagacca cccggcacag gtcaccttta tctgccggta tgcatagggt ggcttccaca
      181 aaaatgcctt ccagcctccg tgctctgcca ggaatgcctc cagatgtcgg gcctcctccc
      241 gggtcacgga aagcgtcaca cggtatgttt tcaggtcagc attcagcccc gccgccatac
      301 gctgcgaata cccgtcacca aaacgcactt cacgcaccga tggctgcgag ttcacctcca
      361 tatccggctt cactttccag cgaaatgttt tcatcgcctg ctccccgata acataccgcc
      421 atcacgcaac tgcagccgga gctcatcctg cgcccccttg cgggccatgt catacaccgc
      481 cttcatcagc tgcggccccg cctgtccgtt ggtgccgtcg ttctgaatca ccacgtgatt
      541 gttctgatta aaattaatgc cttccgtccg ccgcatctgc gccggacttc cggcaccgcc
      601 cacataaccc ccttccgcat agccccgcat caggcggtac aggttgccga cgccaatccg
      661 gctggttgcc tccttcgtga agacaaactc cccgcggtgg acaataccgg caggttcgta
      721 tttgccaccg gttcccgtaa atcctccggt cgcgaaatgg aagttcgccg ccgcagcctg
      781 aat
//
LOCUS       DAZFYC010000423          782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFYC010000423 DAZFYC010000000
VERSION     DAZFYC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.430"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>782
                     /locus_tag="ACHKW5_005682"
     CDS             <1..>782
                     /locus_tag="ACHKW5_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7514800.1"
                     /translation="ALQAANDIATKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDA
                     ILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENA
                     RLGLAAATLQSDMEKAGELAARDRAERDASQLKYTGEAQKGYERLLTPLEKYTARQEE
                     LNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQ
                     AELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAAQRRQLS"
BASE COUNT          209 a          194 c          260 g          119 t
ORIGIN      
        1 gccttacagg cggcgaacga tatcgccacg aaaggctttg atgagcagac ccgtcgcctg
       61 aaagaaaaca tggggacact ggagacctgg gcggataaaa cagggaaggc attcaaatcg
      121 atgtgggatg ccattctgga tatcggtcgt cctgagtcct cagcggatat gctcgccagt
      181 gcgcagaagg catttgatga ggcggataaa aaatggcagt ggtaccagag ccggagccag
      241 cgccgcggta aaacctcttc tttccgggcc aaccttcagg gcgcatggaa tgaccgggaa
      301 aatgcccggc tggggctggc agcggccacg ctgcagtcgg atatggaaaa agccggtgaa
      361 ctggccgcca gggaccgggc cgaacgggac gcatcacagc tgaagtatac cggagaggcg
      421 cagaaggggt atgagcgtct gctgacgccg ctggagaaat ataccgcccg tcaggaagaa
      481 ctgaataagg ccctgaaaga cgggaaaatc ctgcaggcgg attacaacac gctgatggcc
      541 gcggcgaaaa aggactatga agcgacgctg aaaaagccga aacagtccgg cgtgaaggtg
      601 tctgcgggcg atcgtcagga agacagtgct catgctgccc tgctgacgct tcaggcagaa
      661 ctccggacgc tggagaagca tgccggagca aatgagaaaa tcagccagca gcgccgggat
      721 ttgtggaagg cagaaagtca gttcgcggta ctggaggagg cggcacaacg tcgccagctg
      781 tc
//
LOCUS       DAZFYC010000424          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFYC010000424 DAZFYC010000000
VERSION     DAZFYC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.431"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..364
                     /locus_tag="ACHKW5_005683"
     CDS             <1..364
                     /locus_tag="ACHKW5_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514801.1"
                     /translation="PQGPKGDTGAAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGET
                     QIRFRLGPGNIIETNSHGWFPDTDGALITGLTFLAPKDATRVQGFFQHLQVRFGDGPW
                     QDVKGLDEVGSDTGRTGE"
     gene            366..635
                     /locus_tag="ACHKW5_005684"
     CDS             366..635
                     /locus_tag="ACHKW5_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514802.1"
                     /translation="MNILKKIMQRLCGCGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT          205 a          166 c          251 g          159 t
ORIGIN      
        1 tccacagggt ccaaagggag ataccggtgc ggcaggcccg gcaggcccac agggaccgaa
       61 aggagaaaca ggtgcggctg gcccggtggg ggcaaccgga cctcagggac cgaagggcga
      121 cccgggggag acacaaatcc gttttcgtct ggggccggga aacattattg agacaaacag
      181 ccatggctgg ttcccggata cagatggcgc actcatcacc ggactgacct ttcttgcccc
      241 caaagatgcc acacgggttc agggtttttt tcagcatttg caggtcaggt ttggtgacgg
      301 gccgtggcag gatgttaagg ggctggatga agtgggcagt gatacaggca gaacaggaga
      361 atgacatgaa catattaaaa aaaattatgc agcgtctgtg cggttgcgga aagcatgatg
      421 accgtgaaga cggggagtta cttacagcac agctgcgact gggaccggca gacattctgg
      481 agtcagatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      541 tggatgcgga taaaaagcag atacagtgcg tggtaagacc gctgcaaatc ctgcgtgctg
      601 acgggaggtg ggaaaatatt ggcggaatga aatagccgac agcttcacaa aaaccggagt
      661 ccggctccgg tttttgttgt catgtccggt ggatgtttgt taggaatgtt cagacaggtt
      721 tattttgaat ttacacagaa tcttaaacag gctcggccat taatattgtc ggcgggagca
      781 t
//
LOCUS       DAZFYC010000425          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFYC010000425 DAZFYC010000000
VERSION     DAZFYC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.432"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..737)
                     /locus_tag="ACHKW5_005685"
     CDS             complement(33..737)
                     /locus_tag="ACHKW5_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO7514803.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYISSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          208 c          200 g          193 t
ORIGIN      
        1 gatgaggcag ccttttgtct tattcaaagg ccttacattt caaaaactct gcttaccagg
       61 cgcatttcgc ccaggggatc accataataa aatgctgagg cctggccttt gcgtagtgca
      121 cgcatcacct caataccttt gatggtggcg taagccgtct tcatggattt aaatcccagc
      181 gtggcgccga ttatccgttt cagtttgcca tgatcgcatt caatcacgtt gttccggtac
      241 ttaatctgtc ggtgttcaac gtcagacggg caccggcctt cgcgtttgag cagagcaagc
      301 gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc gcgggatctg ccacttcttc
      361 acgttgttga ggattttacc cagaaaccgg tatgcagctt tgctgttacg acgggaggag
      421 atataaaaat cgacagtgcg gccccggctg tcgacggccc ggtacagata cgcccagcgg
      481 ccattgacct tcacgtaggt ttcatccatg tgccacgggc aaagatcgga agggttacgc
      541 cagtaccagc gcagccgttt ttccatttca ggcgcataac gctgaaccca gcggtaaatc
      601 gtggagtgat cgacattcac tccgcgttca gccagcatct cctgcagctc acggtaactg
      661 atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg
      721 cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcacca
//
LOCUS       DAZFYC010000426          761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFYC010000426 DAZFYC010000000
VERSION     DAZFYC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.433"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>761
                     /locus_tag="ACHKW5_005686"
     CDS             <1..>761
                     /locus_tag="ACHKW5_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013026154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO7514804.1"
                     /translation="LREFIPYLEKIWENKWLTNNGPFHQQLENDLCRYLGVEYVSLFN
                     NATIALITAVQSLELTGEVITTPYSFVATTHSLMWNNLNPVFVDVSRDTFNINPSQIE
                     AAITEKTTAIMAVHCYGNPCDVIAIEKIAKKYKLKVIYDAAHAFGVNFKGESLLKYGD
                     LSVVSFHATKVFNTFEGGVIICPNAETKLKIDQLKNFGFEDELTIKSIGINGKMSEVN
                     AAFGLVQLKHVNEAISKRKEINDLYGKLLGNVKGI"
BASE COUNT          275 a          103 c          151 g          232 t
ORIGIN      
        1 actaagagag tttattccct atctggaaaa aatatgggaa aataaatggt taacaaacaa
       61 tggtccattt catcagcaat tagaaaatga tttgtgccgt tatttaggcg tggagtacgt
      121 ctccttattt aataatgcta caattgctct gattacggca gtccaatcat tagaattgac
      181 tggtgaagta attacaaccc cgtattcatt tgtggcaaca actcactcat tgatgtggaa
      241 taatctaaat ccagtatttg tcgatgtcag tagagataca tttaatatca atccgtctca
      301 aattgaagcg gcgataacag aaaaaacaac agccataatg gcagtccatt gttatggtaa
      361 tccttgtgat gtgattgcaa tagaaaaaat agccaaaaag tataaactta aagtgatata
      421 tgatgctgct catgcttttg gggtaaattt taagggggaa agtttattaa aatacggaga
      481 tttatcggtt gttagttttc atgccactaa agtatttaat acatttgaag gtggcgtgat
      541 tatttgccca aatgcagaaa ctaagctcaa aatagatcag ctaaaaaact ttggttttga
      601 agatgagcta acaataaaat caattggtat taatggaaaa atgagtgagg tcaatgcagc
      661 atttggcctt gtacagttga aacatgttaa tgaagctata agtaaaagaa aagaaattaa
      721 tgatttatat ggcaagttat tagggaatgt gaaaggcata t
//
LOCUS       DAZFYC010000427          759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFYC010000427 DAZFYC010000000
VERSION     DAZFYC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.434"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..572
                     /locus_tag="ACHKW5_005687"
     CDS             <1..572
                     /locus_tag="ACHKW5_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO7514805.1"
                     /translation="SPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEK
                     RKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSA
                     RKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEI
                     NRNKQQAGVTAGKPKLDLTNTDWIYGVDL"
     gene            569..>759
                     /locus_tag="ACHKW5_005688"
     CDS             569..>759
                     /locus_tag="ACHKW5_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HFO7514806.1"
                     /translation="MKNIAAQMVNFDREQMRRITNNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNEL"
BASE COUNT          239 a          192 c          193 g          135 t
ORIGIN      
        1 gttcccctaa aatgagggac atccctcaaa acgagggaaa atcccctaaa acgagggata
       61 aaacatccct caaattaggg gattgctatc cctcaaaaca gggggacaca aaagacacta
      121 ttacaaaaga aaaaagaaaa gattattcgt ccgagaattc tggcgaatcc tctgaccagc
      181 cagaaaacga tctttctgtg gttaaaccgg atgctgcaat tcagagcggc agcaagtggg
      241 gaacagcaga agacctgacc gccgcagagt ggatgtttga catggtgaag accatcgcac
      301 catcagccag aaaaccgaat tttgcagggt gggctaacga tatccgcctg atgcgtgaac
      361 gtgacggacg taaccaccgc gacatgtgcg tgctgttccg ctgggcatgc caggacaact
      421 tctggtccgg taacgtgcta agtccggcca aactccgcga caagtggacc caactcgaaa
      481 tcaaccgtaa caagcaacag gctggcgtga cagctggaaa accaaaactc gacctgacaa
      541 acactgactg gatttacggg gtggatttat gaaaaacatc gccgcacaga tggttaactt
      601 tgaccgtgag cagatgcgtc ggatcaccaa caacatgccg gaacagtacg acgaaaagcc
      661 gcaggtacaa caggtagcgc agatcatcaa cggtgtgttc agccagttac tggcaacttt
      721 cccggcgagt ctggctaacc gggaccagaa cgaactgaa
//
LOCUS       DAZFYC010000428          744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFYC010000428 DAZFYC010000000
VERSION     DAZFYC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.435"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..137
                     /locus_tag="ACHKW5_005689"
     CDS             <1..137
                     /locus_tag="ACHKW5_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514807.1"
                     /translation="ATTFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG
                     "
     gene            144..>744
                     /locus_tag="ACHKW5_005690"
     CDS             144..>744
                     /locus_tag="ACHKW5_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7514808.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVG"
BASE COUNT          194 a          170 c          228 g          152 t
ORIGIN      
        1 gagcgacgac gttttttggg ccggaccgga attttgaaaa cccctggttg ctcccggtaa
       61 gcggcacgaa caaccagcgt tgtgtgaatc atatttctga accggatacc gaaattccgc
      121 cgggattcag ggggtgatgt ggcatgaaaa acattgcggc agccggggtt cttgaacgta
      181 ttcgcagact tgcaccacag gcgtcggttc caccgtaccg gacggtggag gagtggcggg
      241 aatggcaact tgctgaagga cgaaaacgca gcgaggagat taaccgccag aatcaccagt
      301 tgcgggtgga aaaaatcctg aatcgttcgg gcatccagcc tctgcacagc aaatgctcgt
      361 ttgcgaatta tcaggtgcag aacgacgggc aaaaatacgc gctgagccag gccaaatcca
      421 tagctgacga actgatgacc gggtgcacga attttgtgtt cagcggtaaa accggcaccg
      481 ggaaaaatca ccttgcagcg gcgatgggta accggctgat ggcgaagggg cgcagcgtga
      541 ttatcgtcac cgtgtctgat gtcatgagcg tgttgcatga cagctacgac aacggcaaat
      601 ccggggaaaa atttttacag gagctttgca gtgttgattt gctggtcctg gatgaaatag
      661 gcgttcagcg ggagacgaaa aacgagcagg tggtattaca ccagataatt gatcgccgga
      721 cagcatcact gtgcagtgtc ggga
//
LOCUS       DAZFYC010000429          734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFYC010000429 DAZFYC010000000
VERSION     DAZFYC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.436"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>734
                     /locus_tag="ACHKW5_005691"
     CDS             <1..>734
                     /locus_tag="ACHKW5_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7514809.1"
                     /translation="EHHGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPP
                     PRSITFHIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQ
                     VLMDKVVHYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDE
                     EETLLRVDAAINKKYCHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGA
                     SVYGKPVITMPKTRNQRGVYLCEVGT"
BASE COUNT          177 a          175 c          229 g          153 t
ORIGIN      
        1 gaacatcatg gctgcgtgat ccatcagtct gaactggacc agagcaacgg gcggtggatc
       61 tgtgaaaaca cgggcatgtg gacccgtgac ggtctgacgt ttttcagcgc ccggggtgat
      121 gaaattccgc cgccgcgctc catcacgttc catatctgga cggcgtacag tccgtttacc
      181 acctgggtac agatagtcta tgactggctg gatgcactga aagatcccaa cggcctgaaa
      241 acctttgtga acaccacgct gggcgagacc tgggaagaag ccgtgggcga aaaactcgat
      301 caccaggtac tgatggataa ggtcgtgcat tacacggcgg cggtacctgc ccgggtggtt
      361 tatctgacgg cgggcattga ctcgcagcga aaccgttttg agatgtatgt ctggggatgg
      421 gcaccgggag aggaagcctt tctggtggat aaaatcatca ttatgggccg tcccgatgag
      481 gaagagacgc tgttacgtgt ggatgcggcg atcaacaaaa aatactgcca tgcagacgga
      541 accgaaatga ccatttcccg tgtctgctgg gacaccgggg ggatcgatgg tgaaattgtc
      601 tatcagaggt caaaaaaaca cggtgttttc cgggtgctgc cggtaaaagg cgcatctgtc
      661 tatggcaagc cggtgatcac catgccgaaa acccgcaatc agcggggcgt gtatctgtgt
      721 gaagtgggga cgga
//
LOCUS       DAZFYC010000430          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFYC010000430 DAZFYC010000000
VERSION     DAZFYC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.437"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..467)
                     /locus_tag="ACHKW5_005692"
     CDS             complement(<1..467)
                     /locus_tag="ACHKW5_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO7514810.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYD"
BASE COUNT          209 a          178 c          179 g          167 t
ORIGIN      
        1 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
       61 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
      121 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
      181 tgcaccttca tgctgtacag catctggttt ttgtcgttct ccccgagttc aaaccaggtc
      241 accacgcctt tcacccggcg ctgtacgtca tcgccctgcc atatcgtcag gtaggccatt
      301 ttgtccagca cctgcgcaaa ttcaagggag agaaactgct ggctgaccag ggagagatcg
      361 agggaaaaga gcgaagagag tgactggttc agatgaaagg aaaccaccgc aaaagcgtcc
      421 ggcggcaggc cgtccacttc cagtgtgaaa cgtaatccgg ttgacatagc aacctccagt
      481 aaaatgaata atgatccgta ccgtacaact gcggataatg tgataagcgg gacgacttca
      541 gacgttatta gggaaggaac agaaaatgct atggttatgt gagaattttg tttaaatatt
      601 actttacaga aacgggtgtc tgttcagtca aaaaaataat cacaagctca acctcttgtt
      661 tcttggcgtt attcaggaca ggtacgcata tcacaacgag taacaaaaac gcaaagttaa
      721 accaatattc ata
//
LOCUS       DAZFYC010000431          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFYC010000431 DAZFYC010000000
VERSION     DAZFYC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.438"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            117..>733
                     /locus_tag="ACHKW5_005693"
     CDS             117..>733
                     /locus_tag="ACHKW5_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7514811.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAENGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSGSGDWRTDGFIVGVGY"
BASE COUNT          163 a          168 c          223 g          179 t
ORIGIN      
        1 ctggttgtgg tcatgaaaaa atccacggca gggatcagta tcagctgacg gtttattaac
       61 ccggacgggc acctcaggag gtgccttttt tattgactga aaacaaagag gtaatcatgc
      121 ggcatttata cgcaacgata ttattgttta ctaccctgct ggcaggaatt gcctttcctg
      181 cacaggctga aaacggacac ggtgcatttt ccgtgggata tgctcaggtt cacccgggcg
      241 gcgtaccggc attgtccggt accggtgcgc gtgcaggtga tttaaaaggg attaatgtga
      301 aataccgtta tgagttcacg gatcacctgg gcggcattgt cgcgctgagt tatgcatcgg
      361 tgaagaaaag tgacacgatg aagacgggtg aaaatacctt ccattatgaa agcctgcgcg
      421 gtcgttatgt cagtctgatg gccggccctg tctggcagct cagtgagcgg gtcagtctct
      481 atggcatggc cgggatggcg tacacccgct ggtctgacag tgttcaggat taccggcgtg
      541 atgaagtgaa accggggtat gtgaaggaga ccaccaccgc cagtgatggc catactgcgc
      601 gtcatctgtc gccggcctgg aatgccggga ttcagttcag tcccgtagag acggtggtta
      661 ttgaccttgc ttatgaaggt tccggcagtg gcgactggcg cactgacggc ttcatcgtgg
      721 gtgtcggcta taa
//
LOCUS       DAZFYC010000432          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFYC010000432 DAZFYC010000000
VERSION     DAZFYC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.439"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>731)
                     /locus_tag="ACHKW5_005694"
     CDS             complement(<1..>731)
                     /locus_tag="ACHKW5_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HFO7514812.1"
                     /translation="ATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSL
                     MNVPLRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQE
                     RRRIWRKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTR
                     LCHDELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSI
                     AETAMYRVKQLFGGSLTLRDYDGQVA"
BASE COUNT          153 a          204 c          195 g          179 t
ORIGIN      
        1 gcaacctgac catcgtagtc acgcagcgtc agtgaacccc cgaacagctg ttttacccgg
       61 tacatcgccg tttccgctat cgagcgacgg ttgtaatctg ttgtccattt ccaccgcgca
      121 ttactcccgg tcattcgctg attcgcaaca gcacggttac ggtctgcata ttcaccgggc
      181 cagtaacccg cgccttttcg gggtgggata agcgcgctga ttttcttacg ccgcagttca
      241 tcgtgacaga gccgggtgtc gtaagcgccg tctgccgatg ctgccctgat ttttctgtga
      301 gtctgccgga taagacccgg gaaggcttct gagtccgtca cattgttcag cgacaggtct
      361 gcacagatga tttcatgtgt gttgctgtca actgccagat gcagcttacg ccagatacgg
      421 cggcgttcct gaccatgctt tttgactttc cactcgcctt caccgaagac cttcagcccg
      481 gtggaatcaa ttaccaggtg tgcgatttca ccccgggtgg gcgttttgaa actgatatta
      541 accgactttg cccgcctgct gacacagctg taatccgggc agcgtagcgg aacgttcatc
      601 agagaaaaaa tggaatcaat aaagccctgc gcagcgcgca gggtcagcct gaatacgcgt
      661 ttaatgacca gcacagtcgt gatggcaagg tcagaatagc gctgaggtct gcctcgtgaa
      721 gaaggtgttg c
//
LOCUS       DAZFYC010000433          730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFYC010000433 DAZFYC010000000
VERSION     DAZFYC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.440"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>730
                     /locus_tag="ACHKW5_005695"
     CDS             <1..>730
                     /locus_tag="ACHKW5_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7514813.1"
                     /translation="MLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVA
                     RFASASGVEVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGA
                     LQAANDIATKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLA
                     SAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEK
                     AGELAARDRAERDASQLKYTGEAQK"
BASE COUNT          172 a          181 c          253 g          124 t
ORIGIN      
        1 atgctgactc tctcaagagc cgggcaggca gcagggctga cgtttaacca ggcgagagag
       61 tcactggcag ccctggtgaa tgccggtgtg cgtggtggtg aacagtttga tgccatcaac
      121 cagagtgtcg cgcgttttgc gtctgcatcc ggtgtggagg tggataaagt cgctgaagcc
      181 ttcgggaagc tgaccactga cccgacgtcg ggactgatgg cgatggcgcg ccagttccgt
      241 aacgtgacgg cagagcagat tgcgtatgtt gcacagctgc agcgttccgg agacgaggcc
      301 ggggcattgc aggcggcgaa cgatatcgcc acgaaaggct ttgatgagca gacccgtcgc
      361 ctgaaagaaa acatgggaac actggagacc tgggcggata aaacagggaa ggcattcaaa
      421 tcgatgtggg atgccattct ggatatcggt cgtccggaat cctcagcgga tatgctcgcc
      481 agtgcgcaga aggcatttga tgaggcggat aaaaaatggc agtggtacca gagccggagc
      541 cagcgccggg gaaagacctc ctctttccgt gccaacctgc agggcgcatg ggatgaccgg
      601 gaaaatgccc gtctggggct ggcagcggcc acgctgcagt cggatatgga aaaagccggt
      661 gaactggccg ccagggaccg ggccgaacgg gacgcatcac agctgaagta taccggagag
      721 gcgcagaagg
//
LOCUS       DAZFYC010000434          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFYC010000434 DAZFYC010000000
VERSION     DAZFYC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.441"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>716
                     /locus_tag="ACHKW5_005696"
     CDS             <1..>716
                     /locus_tag="ACHKW5_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514814.1"
                     /translation="FNDIVPMILALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIY
                     HAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCK
                     ETCKHLLYLSNEDKEKIISDLDKVQVACYYLTILLAFERRTSLTSILATLYKMLISER
                     EVSEYECQLLYLTNPIDVMNILNKYIYYFPNENSPFYTLKIDSALSWDAIDAIRDYSI
                     SDIYLYPEQKTINCVVEIEN"
BASE COUNT          260 a          127 c          112 g          217 t
ORIGIN      
        1 attttaatga cattgtacca atgattctcg cactcgacat caggaacaca tatcgcccgg
       61 ataatgagat ggcattttac tatcatattc attcttttct cactcagata ccggataatg
      121 aggatgatat atatcatgct gcaaggacat atctgcgaaa ttacgttaag ttatgtttat
      181 ccggatacac gccagcgaat gcgcatttca aagatatctt tgatggcgta tatgaattca
      241 ttcgtaatat tcgcaaaaac agtacaccag gaaaaacaaa acttatcgca actatcaaca
      301 catgcaaaga aacctgtaaa catctgcttt atttaagtaa tgaagacaag gaaaaaataa
      361 tttctgactt agataaagtt caggttgcat gttattatct cactatatta ctggctttcg
      421 aaagacgaac ttcattaaca agcatcctgg caactttata taaaatgctg ataagcgaaa
      481 gagaagtttc agaatatgaa tgccagttat tatatttaac caacccaata gatgtaatga
      541 atatactgaa caaatacata tattactttc ctaatgagaa ctcaccattt tatacactga
      601 aaattgacag tgcattatcg tgggatgcca ttgacgcaat acgagactat agtatttctg
      661 atatttatct ttatcctgaa caaaaaacaa taaattgtgt cgttgagatt gaaaac
//
LOCUS       DAZFYC010000435          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFYC010000435 DAZFYC010000000
VERSION     DAZFYC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.442"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>716
                     /locus_tag="ACHKW5_005697"
     CDS             <1..>716
                     /locus_tag="ACHKW5_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO7514815.1"
                     /translation="PRNARISKELLLLAVDRQIEWAKKHFITKDVLENVVSKCDLSSI
                     FNVNKVLQNTILEFVHEINNISSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQFIW
                     GFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYN
                     ALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHIE
                     KNINDNIIYHPSNNANFYSV"
BASE COUNT          241 a           91 c          148 g          236 t
ORIGIN      
        1 cctaggaatg ccagaatatc taaagaattg cttttattag ctgttgatag acagattgag
       61 tgggcgaaaa aacattttat aacaaaagat gtattggaaa atgttgtgtc aaaatgtgat
      121 ttatcatcta tctttaatgt taataaagtg cttcagaata ctattcttga gtttgtccat
      181 gaaattaata atatatcatc tgctcgctgg atgtcaaaat cagaaaagaa taataaacaa
      241 aaagaggcaa tagaaaagtt caaaaaagaa gtatcccata tgaatggcgg gcagcagttt
      301 atttgggggt ttgataaggt tattcaagaa ggcttaagtg gattgattga gttaagtatc
      361 gatattaatg atagtacaaa tcatcgtgat aagtcttctc tttctcctga tgggagagct
      421 gtgttacatt ttttaggtac aatttggaat atggcgatgg gagctgtacc tggttataat
      481 gcattgtctg gtgtaagtag cattttacat agtgctatag taaaagaatc tagcaatatc
      541 tgtgattata ttcagggggc tgtacgtatt ggaatggact ttgttccagg cactcgctct
      601 gacttacata gccgttcgct gcagataaaa tatgaggctt tgaagcatat agaaaaaaac
      661 attaatgata atattattta tcatccgagt aataatgcta atttctattc tgtaat
//
LOCUS       DAZFYC010000436          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFYC010000436 DAZFYC010000000
VERSION     DAZFYC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.443"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..346
                     /locus_tag="ACHKW5_005698"
     CDS             <1..346
                     /locus_tag="ACHKW5_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO7514816.1"
                     /translation="AGWLQTINPNKVKPEIRGKVIQYQEECDDVLYEYWTKGFVVNPR
                     KMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVSKIRTLINEANLLIDFVL
                     ADTGKGKITKAD"
     gene            346..492
                     /locus_tag="ACHKW5_005699"
     CDS             346..492
                     /locus_tag="ACHKW5_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514817.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFSLMLVALFLSMAWMALTSA
                     GLVG"
     gene            504..>712
                     /locus_tag="ACHKW5_005700"
     CDS             504..>712
                     /locus_tag="ACHKW5_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO7514818.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRDNAITYKEQRDKKVSELELA
                     NATITDMQQRQRDVAALDARYSRELA"
BASE COUNT          196 a          145 c          190 g          181 t
ORIGIN      
        1 tgcaggctgg ttacaaacca tcaatcccaa caaagtaaaa cccgaaatcc gcggcaaggt
       61 aatccagtat caggaagagt gtgacgatgt tctctatgaa tactggacga agggttttgt
      121 cgttaatccc cgtaaaatga gtgtgatgga agaactcaat caggcttgcg ctgacatgaa
      181 acgggataaa aacattgcca gtgtgtttgc taccgggctg aatgagtgga aacaggttaa
      241 atccgcgcat gtatcaaaaa tccgtacgct gataaacgaa gcgaatctgc tgattgattt
      301 tgtcctggct gatacaggca aagggaaaat aacaaaggcg gattgatggg gtggctaatg
      361 atatcagata aactcataac gctggtgaag agcctctgtg tacttgtcgg catttcattt
      421 tcactaatgc tggttgctct ttttctttcc atggcctgga tggcgttgac ttcggcaggg
      481 ctggtggggt gagcataaac cgaatgcttt ccgcgtttac cgttattctg ctggtggtct
      541 gtggtgcgct tagtctgggg ctgaatcatt accgcgataa cgccataacc tacaaagagc
      601 agcgcgataa aaaagtcagt gagctggagc tggcaaatgc aaccattact gatatgcagc
      661 agcgccagcg tgatgttgct gcacttgatg ccagatactc gagggaatta gc
//
LOCUS       DAZFYC010000437          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFYC010000437 DAZFYC010000000
VERSION     DAZFYC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.444"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>712)
                     /gene="tssI"
                     /locus_tag="ACHKW5_005701"
     CDS             complement(<1..>712)
                     /gene="tssI"
                     /locus_tag="ACHKW5_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO7514819.1"
                     /translation="SLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSAQIRPS
                     SVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQMDGWR
                     NNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGT
                     ALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRY
                     NPADQDSSCWIRVAQAWAG"
BASE COUNT          109 a          231 c          209 g          163 t
ORIGIN      
        1 tgcctgccca cgcctgtgcc acacggatcc agcacgaact gtcttggtct gccgggttat
       61 aacggtccca gttgaacttc acccgcacgc gaccatgttc gtcgcagaag atttcctcgc
      121 ctgccggtcc tgtcacgaca gcgctctgcg ggccgtcgac cagcggtttc agcaacggct
      181 gtggtcgcca tgttctgtct gccgggatca ccgcaaaatg gttctccagc gccgtccccg
      241 ctccctgccg tcctggcacc gcctgtggct gttcgccgtg cagttcactt gccaccacct
      301 gccattcccg gttcaggttc gcctgcggat gccccgtcag cacaattcgc cgtcccggcc
      361 atatctccgg cgagcggctc attccccgcg cggtttctgc gttgtttcgc cagccgtcca
      421 tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgtccgggg tagtcataca
      481 cttcatactg cgtgcgctgg tagtcctggt gctggccttc ctgttcaaaa cgtccggccc
      541 agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagggcgg atttgtgcgc
      601 tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc ggattccagg
      661 ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg ct
//
LOCUS       DAZFYC010000438          707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFYC010000438 DAZFYC010000000
VERSION     DAZFYC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.445"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>707
                     /locus_tag="ACHKW5_005702"
     rRNA            <1..>707
                     /locus_tag="ACHKW5_005702"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          179 a          162 c          229 g          137 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccac
//
LOCUS       DAZFYC010000439          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFYC010000439 DAZFYC010000000
VERSION     DAZFYC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.446"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..>698
                     /locus_tag="ACHKW5_005703"
     CDS             6..>698
                     /locus_tag="ACHKW5_005703"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFO7514820.1"
                     /translation="MSATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFV
                     SELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMA
                     GDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIA
                     AMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNS
                     APRDTTVTIPAPA"
BASE COUNT          181 a          163 c          195 g          159 t
ORIGIN      
        1 acagtatcag cgcgacaccg aaagccagca tgaagaataa atcctggttc aggatgcaag
       61 ctggggggcc gggtgacgcg gatatttata tttatgacga gattggtttc tggggagtta
      121 ccgcgaagca gtttgtcagc gaactgaatg cactgggtga tatcacccac attaatctcc
      181 atatcaattc accgggtggc gatgtctttg aaggcatcgc catttttaat gccctgaaaa
      241 atcagggggc gaccattacc gtgtatgtgg atggcgttgc cgcctcgatg gcatctgtga
      301 ttgcgatggc cggtgatacg gtcattatgc cggaaaatgc cttcatgatg atccataagc
      361 catggggatt cagtggcggg gatgctgagg atatgcgcag ttatgccgat ttgctggata
      421 aagtcgaatc ggtactgttg ccagcctatg cgcagaaaac cggaaaaacc accgatgaaa
      481 ttgccgccat gctggcggat gaaacctgga tgtccggtgc cgaatgtctg gcacacggat
      541 ttgctgacca ggtgacaccc gctgttgagg caatggcatg tattcagtca aaacgtacag
      601 aggaatttaa aaagatgccg gaatccatcc gaaacatgat tactccgcca cgcaacagtg
      661 ccccgcgtga taccacagtg acaatccctg caccggcg
//
LOCUS       DAZFYC010000440          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFYC010000440 DAZFYC010000000
VERSION     DAZFYC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.447"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..604
                     /locus_tag="ACHKW5_005704"
     CDS             <1..604
                     /locus_tag="ACHKW5_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7514821.1"
                     /translation="QYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSP
                     VNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYLPMNPISS
                     NDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSY
                     MNGMEKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            606..>698
                     /locus_tag="ACHKW5_005705"
     CDS             606..>698
                     /locus_tag="ACHKW5_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO7514822.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSN"
BASE COUNT          254 a           97 c          153 g          194 t
ORIGIN      
        1 gcagtatgat gaggagtccg gcctgtatta caaccgccac cgctattatg acccgctgca
       61 ggggcggtat atcactcagg atccgattgg gctgaagggg ggatggaacc tgtatacata
      121 tccgctgagc ccggtgaata gcatggatcc attaggatta tatgaattta aatcaaaaaa
      181 tatagatgat attggaatat ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa
      241 taaagaatat ggtggactaa tatgtaagaa gcaaggtgaa tatttaccca tgaatccgat
      301 aagttcaaat gataatgata gtgtagactt gcgaaatata aaatgccctg aaggttcaga
      361 gagagtaggc gattatcaca ctcacggttt ttactctgac gataaaggaa ataaagtaac
      421 aaaagaaaat gatgtttatg atagtctaaa tttttcaagc aaagatttaa cgaattctta
      481 tatgaatgga atggaaaaaa aagaatacag tagttacttg ggaacaccaa ataacaccta
      541 tctaaaatat aatcccaaag ctaaagggaa tggagttaca attatcaggc aagggagtaa
      601 ttaaaatgaa ggcaatacaa tgttgttatt tccttgcttt agtctccagt catttaatgg
      661 ctggagttat gggtgatgat aatatatttt taagtaat
//
LOCUS       DAZFYC010000441          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFYC010000441 DAZFYC010000000
VERSION     DAZFYC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.448"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>692
                     /locus_tag="ACHKW5_005706"
     CDS             <1..>692
                     /locus_tag="ACHKW5_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7514823.1"
                     /translation="PTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSK
                     STLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTH
                     IRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLAS
                     RPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAH
                     LLMENLAMVNPNL"
BASE COUNT          147 a          186 c          212 g          147 t
ORIGIN      
        1 ccgacattcg gggagtcctg tgcaccgtgg gtgtttgacc tggtggcggc cctgtttggc
       61 tcctacgatg cgcagaccgg tgttcgccat atcaaggaag tgtttatcct tatccccaag
      121 aaaaacagta agtccacgct ggctgcgggg atcatgatga cggcgctgtt actgaactgg
      181 cggcaggcgg cgggctacac cattctggcc ccgaccgtgg aggtggcggc taacgccttc
      241 aaccctgcca gggatatggt acgacgggac gatgatctgg atgacctctg tcaggtgcag
      301 acacatatcc ggaccatcac ccatcgggtg acggacacca ccctgaaggt ggtggcagcc
      361 gatccgaata cggtgtccgg tatcaagtcc gtggggacac tgattgatga actgtggctg
      421 tttggcaagc agtacaaggc ggaagacatg ctacgtgaag ccatcggcgg gcttgcctcc
      481 cgtccggaag ggtttgtggt gtatacgacc acccagtcga atgaaccgcc cgccggggtg
      541 ttcagacaga aactgcagta tgcccgggat gtccgtgacg gcaaaattca tgatccgcac
      601 tttctgccgg tgatttttga gcatcctcct gaaatggtgg aaagcggggc tcacctgctg
      661 atggaaaacc tcgccatggt taacccgaat ct
//
LOCUS       DAZFYC010000442          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFYC010000442 DAZFYC010000000
VERSION     DAZFYC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.449"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..264
                     /locus_tag="ACHKW5_005707"
     CDS             <1..264
                     /locus_tag="ACHKW5_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514824.1"
                     /translation="VLKKLMQRLCGYGKHDDREHGELLTAQLRLGPADILESDENGII
                     PEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENVGGMK"
     gene            complement(391..>691)
                     /gene="tccP2"
                     /locus_tag="ACHKW5_005708"
     CDS             complement(391..>691)
                     /gene="tccP2"
                     /locus_tag="ACHKW5_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO7514825.1"
                     /translation="PARNMAEHIPPAPNWPAPTPPVQNEQSRPLPDVAQRLMQHLAEH
                     GIQPARNMAEHIPPTPNWPAPTPPVQNEQSRPLPDVAQRLMQHLAEHGINTSKRS"
BASE COUNT          175 a          143 c          205 g          168 t
ORIGIN      
        1 gtactaaaaa aacttatgca gcgtctgtgc gggtacggaa agcatgatga ccgtgaacac
       61 ggggagttac ttacagcaca actgcgtctg gggccggcag acatcctgga gtcagatgag
      121 aatggtatta ttccggagca ggccagggta atcacgcagg tggtgatact ggatgcggat
      181 aaaaagcaga tacagtgtgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg
      241 gaaaatgttg gcgggatgaa atagcccgac agcttcacaa aaaccggagt ccggctccgg
      301 ttttttgttg gttagatgta atctgacaga tacctgtata aataaccggt aactgtcagg
      361 tcagagctaa tacaggtaat tatattataa tcacgagcgc ttagatgtat taatgccatg
      421 ctctgcaaga tgctgcatca gacgctgagc cacatcaggc agaggtcttg attgttcatt
      481 ttgtaccggc ggcgttggcg caggccagtt aggtgtcgga ggaatatgtt cagccatatt
      541 ccgggctggt tgaatgccat gctctgcaag atgctgcatc agacgctgag ccacatcagg
      601 cagaggtctt gattgttcat tttgtaccgg cggcgttggc gcaggccagt taggtgccgg
      661 aggaatatgt tcagccatat tccgggctgg t
//
LOCUS       DAZFYC010000443          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFYC010000443 DAZFYC010000000
VERSION     DAZFYC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.450"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..141
                     /locus_tag="ACHKW5_005709"
     CDS             <1..141
                     /locus_tag="ACHKW5_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7514826.1"
                     /translation="GVRFGGDGGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPA
                     SG"
     gene            122..>686
                     /locus_tag="ACHKW5_005710"
     CDS             122..>686
                     /locus_tag="ACHKW5_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7514827.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSL"
BASE COUNT          132 a          168 c          235 g          151 t
ORIGIN      
        1 ggcgtccggt tcggagggga cggagggcgc gatatttcac cttctgcgga tgtggtggat
       61 gtcagcgagg atgatgttgc attaatgatg gcttcagcgg ggatttccgg aggtgtgaga
      121 tatgtcccag ccagcgggtg atctggttat tgatttgagt ctggatgcgg cccggtttga
      181 tgaacagatg gcccgggtac gccgtcattt ttccagtctg gaggcggatg ccagaaaaac
      241 cgccagtact gttgaacagg ggctgagccg acaggcgctg gcggcacaaa aagccgggat
      301 atcagtcgga cagtataagg ctgccatgcg cacactgccc gcacagttca cggatattgt
      361 cactcagctt gccggtggtc agaatccctt ccttatcatg ctgcagcagg gggggcagat
      421 cagcgattcg ttcggtggac cgctcagcct gcttaccctg ctgaaggagg aacttctcgg
      481 gatcagggat gcctctgaat catcagagga gtcgctgtca gatacggcaa atgcactggc
      541 tgaaaatgcc cggaatgccg gtgagctggg acgatttatg tcggtggccc gtgtggcggc
      601 aggtggcggg gttgccgtac tggccgcgct tgctgccgcc gcctggcagg cagagcaggc
      661 tgaccgggcc ttattgcgtt cactga
//
LOCUS       DAZFYC010000444          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFYC010000444 DAZFYC010000000
VERSION     DAZFYC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.451"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..346
                     /locus_tag="ACHKW5_005711"
     CDS             <1..346
                     /locus_tag="ACHKW5_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO7514828.1"
                     /translation="AGWLQTINPNKVKPEIRGKVIQYQEECDDVLYEYWTKGFVVNPR
                     RMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVSKIRTLINEANLLIDFVL
                     ADTDKGKITKAD"
     gene            346..495
                     /locus_tag="ACHKW5_005712"
     CDS             346..495
                     /locus_tag="ACHKW5_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514829.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            503..>684
                     /locus_tag="ACHKW5_005713"
     CDS             503..>684
                     /locus_tag="ACHKW5_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7514830.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDV"
BASE COUNT          191 a          136 c          184 g          173 t
ORIGIN      
        1 tgcaggctgg ttacaaacca tcaatcccaa caaagtaaaa cccgaaatcc gcggcaaggt
       61 aatccagtat caggaagagt gtgacgatgt tctctatgaa tactggacga agggttttgt
      121 cgttaatccc cgtcgaatga gtgtgatgga agaactcaat caggcttgcg ctgacatgaa
      181 acgggataaa aacattgcca gtgtgtttgc taccgggctg aatgagtgga aacaggttaa
      241 atccgcgcat gtatcaaaaa tccgcacatt gataaacgaa gcgaatctgc tgattgattt
      301 tgtcctggct gatacagaca aagggaaaat aacaaaggcg gattgatgga gtggtggcta
      361 atgatatcgg ataaactcat aacgctggcg aagatcctct gtgtaatcgt cggcatttca
      421 tttttagtca tgctggttgc cattttcttt tccaccgctt ggcgagtcct gacgttatcg
      481 ggactggtgg ggtgaaagag agatgaaccg tgttctgtgt gtggtgatta ttgtcctgct
      541 ggtagcctgt ggtgcgctta gtctggggct gaatcattac cgcgataacg ccataaccta
      601 caaagagcag cgcgataaaa aagtcagtga gctggagctg gcaaatgcaa ccattactga
      661 tatgcagcag cgccagcgtg atgt
//
LOCUS       DAZFYC010000445          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFYC010000445 DAZFYC010000000
VERSION     DAZFYC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.452"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..675)
                     /locus_tag="ACHKW5_005714"
                     /pseudo
     CDS             complement(1..675)
                     /locus_tag="ACHKW5_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          217 a          146 c           94 g          218 t
ORIGIN      
        1 tgctatctga aagagcagtg aggaaatttt tcacctcatt aatcctcttc tctgggtcgg
       61 tgtctaaaac aaggcgtgcc ttgttatcat taaatataaa ctcgggtaaa tgcagtatgc
      121 attttttgtc attcaagcaa ctatcaataa attatttttt aaaaaacaga aagttccatg
      181 acataagact accctccccc tgaattttgt tgtcagattg tagatcaacg ccaaataacg
      241 tctttacatc ttccctgaga ttatagaatg ccgtatcaac aagagcctcg atcgtttgtt
      301 tatcaacaat tttaataaac tcttcgtata cttgcttttg cgtcttatgg ctgcccagcg
      361 cccatgaata ctcatgttct tttaaaacca gtatggccct taacagacaa tagccatcgc
      421 catctactgg agcgtatttt aatccataag ttttattaat ttcactctga ataaaagata
      481 taccagacaa atcaatacca gaatctaaat cagatattcc cggcgtattt atctgttctg
      541 gctggatact catattttca tcatctaaac aatactcaga aaaaacatca tcacacgcag
      601 taatgttttc aatatattcc ataaagccat accaataata acctttaaaa tctgtgatag
      661 actctgaacc tctgc
//
LOCUS       DAZFYC010000446          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFYC010000446 DAZFYC010000000
VERSION     DAZFYC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.453"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..622
                     /locus_tag="ACHKW5_005715"
     CDS             <1..622
                     /locus_tag="ACHKW5_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO7514831.1"
                     /translation="FDKRKMEFTYPGANKSHLQSSNSDVVAAAAISIAATEIKTILPD
                     DLTPGKYNKIYLSGHGSAGLPLLKCGDEFLSPADIVDRIVQHNLHEIDDIRLTSCNSA
                     NIIKNKDFSPDEIDKSSNINNGWLARTLFGQKKSLAEHVYAEFESRGINVSISGYHGI
                     GVFYVPEHGKPTTHLRSTTVPATPEYTVRRSDYRATFGRTQPIDID"
BASE COUNT          226 a          135 c          126 g          184 t
ORIGIN      
        1 ttttgataaa aggaaaatgg agtttacata tcctggagca aataaaagcc atctacaatc
       61 aagtaatagc gatgttgttg ctgctgcagc tataagtatt gcagcgacag agataaaaac
      121 catcctgcca gatgatttaa caccaggaaa atacaacaaa atttatctat ctgggcatgg
      181 ttctgctggt ctacctcttc ttaagtgcgg agatgaattt ttatcaccgg cagatattgt
      241 cgaccgcatt gttcaacata atcttcatga aatagatgat atcagattaa catcctgtaa
      301 ctcagccaac ataataaaaa acaaagactt ctctcctgat gaaatagata aatcctcaaa
      361 tattaataac ggctggttgg ccaggacatt atttggtcaa aagaagtctt tagcagaaca
      421 cgtctatgcc gagtttgaaa gtcgtggaat taacgtttct atatcaggtt accatggcat
      481 tggcgttttt tatgtaccag agcatggtaa accaacaacg catctacgct ccacaactgt
      541 tcctgcaaca cctgaatata ctgtaagaag aagcgactac agagccactt ttggtagaac
      601 tcaacccata gatattgact aatttagtca gcatatacac ttgatggtta ggaaactgaa
      661 ccgccccggg a
//
LOCUS       DAZFYC010000447          638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFYC010000447 DAZFYC010000000
VERSION     DAZFYC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.454"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(423..>638)
                     /locus_tag="ACHKW5_005716"
     CDS             complement(423..>638)
                     /locus_tag="ACHKW5_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010670613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HFO7514832.1"
                     /translation="GSIAESWRQQPCTPSYCCDWEAATMLRAFPLAKKGEGRARLPSL
                     YASFGKLGETPTHEDIIDNNRSINWPV"
BASE COUNT          181 a          146 c          153 g          158 t
ORIGIN      
        1 ctgacaggtt cataatcaac gcaaaccaga gctgtacagg cttgggcgcg gctttcaaac
       61 cagtcgtgat cacggcaatc aattttgaac tctgcttaac ggacatttct gtataaccct
      121 tacggcaacg aaaaacgcga agttaaaatt ttagaaaccc aaaaacgtga catgactaag
      181 tttagatttc agggggggag atcaaaaaat ttcgctctgt gccagagcgg acattcatgg
      241 agctggttca ttaccaatga ggttgggctt ttgaggataa atcaatgatc agacgccaac
      301 gtaaatcaaa agcaaccctg gaacggaatt gctaatccag tttctgacca tcgatttttc
      361 taaaaagtgt gcgtttgcta ctacttaggt aggtgcagct ttcttaatca ccggcagcca
      421 cgttatacag gccagttgat ggatcgattg ttatcaatga tatcttcatg agtcggtgtc
      481 tcacccagct taccgaagct ggcataaagt gaaggcagac gggcccgtcc ttctcccttt
      541 ttcgccagag ggaaagcgcg aagcatggtg gcagcctccc agtcacaaca ataggatggt
      601 gtgcacggct gctgacgcca tgattcagcg atagagcc
//
LOCUS       DAZFYC010000448          637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFYC010000448 DAZFYC010000000
VERSION     DAZFYC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.455"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>637
                     /locus_tag="ACHKW5_005717"
     CDS             <1..>637
                     /locus_tag="ACHKW5_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFO7514833.1"
                     /translation="PDSRILSSVRDAVASSDNGAQVKVGNRTYRVVVTDNKFCVTRES
                     HSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRTMERGAVHSNRPDLPPVDYAPPEL
                     PSVDYNRLPVPGNVIGKGGNAVVYEDAEDATKVLKMFTASQSNEEVTNEVRCFNQYYG
                     AGSAEKIYGDNGDIIGIRMDKINGESLFNISSLPAQAEHAIYDMFDRLEQKG"
BASE COUNT          189 a          100 c          170 g          178 t
ORIGIN      
        1 tcctgacagt cgtattttat cctctgtaag ggatgcagtt gctagctcgg ataatggggc
       61 gcaagtaaag gtgggcaaca gaacatatcg tgttgttgtc actgataata agttttgcgt
      121 tacaagagaa agtcatagtg gttgctttac taatctgttg cacaggctgg gatggcctaa
      181 gggagagatt agcaggaaaa ttgaggtgat gctgaattca tcgccagtga ataggactat
      241 ggaaagaggt gctgttcatt cgaatagacc tgatttacct cctgttgatt atgcaccgcc
      301 agagttaccg agtgtggact ataacaggtt gcctgtgcct ggtaatgtta ttggcaaagg
      361 gggtaacgct gtagtatatg aagatgctga ggatgcaaca aaagtcctga agatgtttac
      421 tgcatctcaa agcaatgaag aggtaacaaa cgaagttcgt tgcttcaacc aatattatgg
      481 tgccgggagt gcagaaaaaa tatatggcga taatggcgat attattggta ttagaatgga
      541 taaaataaat ggagaatcgc tttttaatat ttcgtccttg ccggcacagg ctgagcatgc
      601 catttatgat atgtttgaca ggcttgagca aaaagga
//
LOCUS       DAZFYC010000449          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFYC010000449 DAZFYC010000000
VERSION     DAZFYC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.456"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>634
                     /gene="wzx"
                     /locus_tag="ACHKW5_005718"
     CDS             <1..>634
                     /gene="wzx"
                     /locus_tag="ACHKW5_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115209107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O123/O186 family O-antigen flippase"
                     /protein_id="HFO7514834.1"
                     /translation="LVVIGKSFSASTLGQFTQANLLSNTPAMTLTTVVQRVTYPLLSN
                     VNNAKGNIDEIYLRTLRLTAAAVFPVMFLLAIIAKPFVVLFLGQQWEPVAELMSILCI
                     GYCLYPVHAINLNLLQVKGRTDLFLKLEIIKKTLITVILIVTIPFGVKIICIGIFAQY
                     YISLLINTYYTGKLSSLSAIAQIKALLPIWLMASISSAISWFLIPREIFS"
BASE COUNT          209 a          100 c          115 g          210 t
ORIGIN      
        1 atttggtagt tatagggaag tcctttagcg ctagcacact gggccaattt acacaagcta
       61 atttattatc aaatacgccg gccatgacgt taacgacagt tgtacaaaga gttacctatc
      121 cattattaag taatgtgaat aatgctaagg gaaatattga cgagatatat cttaggacat
      181 taaggcttac tgccgcagcc gtttttccag taatgttcct attggcaata attgctaaac
      241 cttttgtcgt tctatttctt ggccaacaat gggaacctgt tgcggaatta atgagcatac
      301 tatgtatagg atactgttta tatccagtac atgctattaa tctaaactta ttacaggtaa
      361 aaggacgaac tgatttattt ttgaagttag aaataataaa aaaaactctc atcacggtta
      421 ttttaatagt aacaatacca tttggtgtta aaataatatg tataggtatt tttgcacagt
      481 attatatatc cttgttgata aatacatact atacagggaa actcagcagc ttaagtgcaa
      541 ttgcacagat aaaggcatta ttgccaattt ggttaatggc atctatcagt tcggcaatta
      601 gttggttctt aataccaaga gagatatttt cgga
//
LOCUS       DAZFYC010000450          632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFYC010000450 DAZFYC010000000
VERSION     DAZFYC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.457"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..521
                     /locus_tag="ACHKW5_005719"
     CDS             <1..521
                     /locus_tag="ACHKW5_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO7514835.1"
                     /translation="ATEMKTILPDDLTPGKYKKIYLSGDGSAGLPLLKCGDEFLSPTD
                     IVDRIVQHNLHEIDDIRLTSCNSANILKNKDFSPDEIEKSANMNNGWLARALFGQKSS
                     LAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRSDYR
                     ATLGRTQPIDIE"
BASE COUNT          202 a          132 c          131 g          167 t
ORIGIN      
        1 cagcgacaga gatgaaaacc atcctgccag atgatttaac accaggaaaa tacaaaaaaa
       61 tttatctgtc tggggatggt tctgctggtc taccacttct taagtgtgga gatgaatttt
      121 tatcgccgac agatattgtc gaccgcattg ttcaacataa tcttcatgag attgatgata
      181 tcagattaac atcctgtaac tcagccaaca tattaaaaaa caaagacttc tctcctgatg
      241 aaatagagaa atccgcaaat atgaataacg gctggttggc tagggcatta tttggtcaaa
      301 agagttcttt agcagaacac gtctatgccg agtttgaacg tcgcggaatt aacgtttcta
      361 tatcaggtta ccatggcact ggcgtttttt atgtaccaga gcatggtaaa ccaacaacac
      421 atctacgctc cacaactgtg cctgcaacac ctgaacatac tgtaagaaga agcgactaca
      481 gagccacttt gggtagaact caacccatag atattgagta attgagccag catatacact
      541 tgatggttag gaaacagaat cattgtttta aacatttcct ggccattaat attgtcggcg
      601 ggagcatatc caggactggc cggcaaaccg gg
//
LOCUS       DAZFYC010000451          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFYC010000451 DAZFYC010000000
VERSION     DAZFYC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.458"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..448
                     /locus_tag="ACHKW5_005720"
     CDS             <1..448
                     /locus_tag="ACHKW5_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514836.1"
                     /translation="WTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFIS
                     MMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKET
                     ETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            548..629
                     /locus_tag="ACHKW5_005721"
                     /pseudo
     CDS             548..629
                     /locus_tag="ACHKW5_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          200 a          115 c          109 g          205 t
ORIGIN      
        1 ctggacaaga aaacgtattc ctgaactttc agcacttcca ccttcattaa gaaagaaaaa
       61 ttttactgac gcatataatg cagcatcttc tcatatcgaa tactggatag gtgctggcgt
      121 ctcatttatc tcgatgatga ttttatccag agtatatgac tttcttttac ccgcccaaga
      181 tactttcccg ggtgatatca tcagaagttt atgtgttgtg tgtccatcca tattaatctg
      241 gtttcagttt tcggtatatg tcatgaggaa atattacaga catattctgg tgcgaggaaa
      301 agaaaccgaa accatttcag aaaggttgat acgtgaggct gatacacgag aatatgaact
      361 atggcgtccg gtccgtcgct ttttctccat tgttttcctc attgttctat taggttgcat
      421 tcattcactg ataacaacta taaaataaaa gttatggctg ttctgataca ggaaaataaa
      481 cgttcattct atggttacct cttcctgtta tatagaagaa ttttaaacga caagtaaggg
      541 aatataaatg aaaataacaa aaaataaaaa tgcaactata aaaaacagtc atcagtcggc
      601 tgcttttcaa tgtttacctt atcattttc
//
LOCUS       DAZFYC010000452          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFYC010000452 DAZFYC010000000
VERSION     DAZFYC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.459"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            290..>629
                     /locus_tag="ACHKW5_005722"
     CDS             290..>629
                     /locus_tag="ACHKW5_005722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514837.1"
                     /translation="MSTENLQPEHRDDGLPAKIAALASLQAIIARMASNSQLMKTWAI
                     TIVTGFIAIKSTFGGLGYLSYLVPILICISFSFLDSYYLSQEKIFRDVYNKLAAIPVG
                     NEMMYLDFKGE"
BASE COUNT          210 a          126 c           97 g          196 t
ORIGIN      
        1 tgaaatgtat taaaaatgat tcatcagcta gccacagtac tttcttacat acgacgtgcc
       61 ttcactttca actcccgcca actcaccaat caggacagga aagcaggcag aaatccctca
      121 aaaaccaaaa aaacaatttt tatactgaaa atcaataagt taaacaatga aaattatcct
      181 ccccatcgat tttcacctca tcaaaaagag ttttttctaa tatagaatcc tttgtttcat
      241 cagttttttt gttataaagt atacttttga tcaaagtctg aggatattca tgagtacaga
      301 aaacttacaa ccagaacatc gtgatgacgg attacccgct aaaatagcag cattagcaag
      361 tctgcaagcc ataattgcaa ggatggcttc aaattcacaa ttaatgaaaa cgtgggccat
      421 cactatcgtt actggattca tagctatcaa gtcgactttt ggtggacttg ggtatctatc
      481 ttatctagtg ccgattctta tttgcatttc attctccttt cttgactctt attatctttc
      541 tcaagaaaag atatttagag atgtttataa taaattagcg gccattcccg ttgggaacga
      601 aatgatgtac ctagatttca aaggcgaaa
//
LOCUS       DAZFYC010000453          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFYC010000453 DAZFYC010000000
VERSION     DAZFYC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.460"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..266)
                     /locus_tag="ACHKW5_005723"
     CDS             complement(<1..266)
                     /locus_tag="ACHKW5_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7514838.1"
                     /translation="MGRADWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDMHFSGLMY
                     AVLSLFFCDPDMHPADFSLFAPEAEEGQAETPDENDVLMQKAAGL"
     gene            complement(293..>628)
                     /locus_tag="ACHKW5_005724"
     CDS             complement(293..>628)
                     /locus_tag="ACHKW5_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7514839.1"
                     /translation="KFVSDAEQQKTTKHDVVRINQRYLETASLLVAMSLWHSHSLKGT
                     LASPETEMQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEPTGKTEATEPV
                     TSKKHSKAS"
BASE COUNT          134 a          182 c          158 g          154 t
ORIGIN      
        1 aggcccgccg ccttctgcat cagtacatca ttttcgtccg gcgtctccgc ctgtccttcc
       61 tctgcctccg gagcgaacag gctgaaatcc gccggatgca tatccggatc gcaaaaaaac
      121 aggctgagta cggcgtacat cagcccggaa aaatgcatat ccagctgggt atcctgaaaa
      181 taatgcgtgc agtaaaaacg tcgccagtcg gcatattcgg tggatgtcat cccggcaagc
      241 atggcgcgcc agtcggctct ccccatctca cgcgccagtt tcaggacaaa gttcagctcg
      301 ccttcgaatg cttttttgat gttaccggct cagtcgcttc tgttttcccg gttggttcag
      361 gatcggcatc gtgccggtta tccagcatac ctgaaagata aagcacccgg ttcgttgcct
      421 gattcagtgc atcagcaggc catcccagca tcacttcacg gcggatctgc tgcatctctg
      481 tctccggaga ggccagagtg cctttgaggg aatgggaatg ccatagcgac atcgccacaa
      541 gcagggatgc cgtttccaga tatcgctggt taatgcgcac gacatcatgc ttcgttgtct
      601 tctgttgttc tgcgtctgaa acaaactt
//
LOCUS       DAZFYC010000454          618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFYC010000454 DAZFYC010000000
VERSION     DAZFYC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.461"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            136..>618
                     /locus_tag="ACHKW5_005725"
     CDS             136..>618
                     /locus_tag="ACHKW5_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7514840.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     F"
BASE COUNT          167 a          122 c          190 g          139 t
ORIGIN      
        1 acacttcttg gtgagaggat tatggaccgc atgaccatga acggtggtcg atgagtgacg
       61 tttaactggg atagctggcg tccaaatgtc agcaatatga gggttgtgaa gtaattttgt
      121 ccggaggaaa ttttaatgga aaccgtatct gacgcactga aagcactgaa aaaagcctct
      181 tcacatgtgg tggcagctcg ccttggaatc agtcgtgaag aggctgtcaa cgagctgtgg
      241 gaactcaaaa taaatggcgt cgttgataaa actggtcaca cctggtttct ggctggcgaa
      301 ggtgaatccc gggtaaccga agagcggcca gtaaaatctg aagcacagga tatgctgacc
      361 ggggaggtcg aacaaaaagt taccgcagac atgatgattg agtttatcgg tcaggatggg
      421 gctaaaacgt gtgaggaact ggcgggtaag ttcggtgtca gtactcgcaa ggttgcttcc
      481 acgctggcgg tggtaaccgc aacggggcgg ctggcacgcg ttaatcagaa cggtaaattt
      541 cgttactgca tgccgggcga taatttacca gcagagccga aagccgcgct ggtaacggaa
      601 agtgatggta aggccttt
//
LOCUS       DAZFYC010000455          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFYC010000455 DAZFYC010000000
VERSION     DAZFYC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.462"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..614
                     /locus_tag="ACHKW5_005726"
                     /pseudo
     CDS             1..614
                     /locus_tag="ACHKW5_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          162 a          172 c          164 g          116 t
ORIGIN      
        1 gacaaaaaca gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag
       61 tgaagccctg aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct
      121 gtatgaatca cagctctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga
      181 acgtgaactg gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga
      241 tgaagagctg gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag
      301 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc
      361 cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt
      421 cagcagttcc gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag
      481 cgttacggtg ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta
      541 aaaaccgtgg cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc
      601 agcccggtca gcta
//
LOCUS       DAZFYC010000456          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFYC010000456 DAZFYC010000000
VERSION     DAZFYC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.463"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..>612
                     /gene="wzx"
                     /locus_tag="ACHKW5_005727"
     CDS             59..>612
                     /gene="wzx"
                     /locus_tag="ACHKW5_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115209107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O123/O186 family O-antigen flippase"
                     /protein_id="HFO7514841.1"
                     /translation="MNSNVKKNISAVNGLKWSAIERICSQGIQLLLMIVLARQLGPGA
                     FGLIGMLTIFITIGQVFIDSGFSAALIRKNERTESDYATVFYFNMTVAILFYAVLFFC
                     APFIAEFYKRNELIELTRVLGLTIIISAFIIVQRIQLSVILDFKTQAISSLSSVIISG
                     GGALLMAYNGFGVWSLVIQTITMGL"
BASE COUNT          192 a           84 c          123 g          213 t
ORIGIN      
        1 agttaattta acaactaaga aggtgagtat ctatctctaa gtgttattga gaaggtatat
       61 gaattcaaat gtaaaaaaaa atattagcgc tgtaaatgga ttgaagtgga gtgcgatcga
      121 aagaatatgt tcacaaggta tccagctact tttaatgata gtcttggcta gacaattagg
      181 gcctggtgca tttggcctta ttggaatgct gacaatattt attacaatag gtcaggtctt
      241 tattgatagt ggttttagtg ccgctctcat tcgaaaaaat gaaagaacag aatcagacta
      301 tgcaactgtt ttttacttta acatgacagt tgccattctg ttttatgcag tgttattttt
      361 ttgcgcccca ttcatagctg aattttataa gcgtaacgaa ttaattgaat taacaagagt
      421 tctgggttta acaataataa taagtgcttt tattattgtt caacgaatac aattaagtgt
      481 cattttggat ttcaaaactc aagctatatc gtcattatcc agtgtcataa tctcaggagg
      541 gggtgcacta ttaatggcat ataatggttt tggtgtatgg tcgttagtta tacagactat
      601 taccatgggg ct
//
LOCUS       DAZFYC010000457          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFYC010000457 DAZFYC010000000
VERSION     DAZFYC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.464"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..67
                     /locus_tag="ACHKW5_005728"
     CDS             <1..67
                     /locus_tag="ACHKW5_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7514842.1"
                     /translation="AIKAEYQQKLKDLRNSRSEAA"
     gene            64..>612
                     /locus_tag="ACHKW5_005729"
     CDS             64..>612
                     /locus_tag="ACHKW5_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO7514843.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFI"
BASE COUNT          196 a          127 c          152 g          137 t
ORIGIN      
        1 ggcgatcaag gcagagtacc aacagaaact caaagacctg cgaaacagca gaagtgaggc
       61 tgcatgaata tctacgaaag aattgatggc agcaaatacc gaaatatttg ggtagctggc
      121 gacctgcacg gatgctacac gaacctgatg aacaaactgg atacgattgg attcgacacc
      181 aaaaaagacc tgctcatctc ggttggcgat ttggttgatc gcggtacaga gaacgtcgaa
      241 tgtctggaat taatcacatt cccctggttc agagctgtgc gtggaaacca tgagcaaatg
      301 atgattgatg gcttatcaga gcgtggaaac gttaatcact ggctgcttaa tggcggtggc
      361 tggttcttta atctcgatta cgacaaagag actctggcta aagttcttgc ccataaagca
      421 gaagaacttc cgttaatcat cgaactggtg agcaaagata aaaaatatgt catttgccac
      481 gccgattatc cttgtgataa atacgagttt ggaaagccag ttgatcatca gcaggtaatc
      541 tggaaccgcg aacgaatcag caactcacaa gacgggatcg tgaaagaaat caaaggcgcg
      601 gacacgttca tc
//
LOCUS       DAZFYC010000458          610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFYC010000458 DAZFYC010000000
VERSION     DAZFYC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.465"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..99)
                     /locus_tag="ACHKW5_005730"
     CDS             complement(<1..99)
                     /locus_tag="ACHKW5_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7514844.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADG"
     gene            complement(96..458)
                     /locus_tag="ACHKW5_005731"
                     /pseudo
     CDS             complement(96..458)
                     /locus_tag="ACHKW5_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373978.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          149 a          198 c          123 g          140 t
ORIGIN      
        1 cccatccgcc agcaccgaca tgattctgct ggtgaaatcc accatcacca ccagaaacag
       61 caggagtgca gccacagcca ggcgcagttt taccgtcaca ggtgattctc cagacgaagc
      121 cccagaacac cggcaatctc ttccagcacc ttgcgctctt ccggctcaat ttcgccgtct
      181 gcctccgcaa tggccaccgc cacatccagc acatcttccg cttcacgcgt atcgcgtttc
      241 acatcctcga tctcacgtaa cgccgcacga cgaccaattt taaagttcgt atccagctga
      301 ccgataatgg ttgcgctaat cgcattaatt tctgacgtaa acgcggacag cgctggctga
      361 ttacgcagta cctgttcgat cttcgctttc taggaagcct cacattcacc atttgcacag
      421 gccaccaggt atgcggcgtt aatcaccgcc tgtgccagat cgcgtttctc aaactttcct
      481 ttttccggtt aacgtgacac accaataact cttgtcgaaa aagccagcaa gctgaaagac
      541 cggtattcac aaccaccagc gcgtttactg tactggcgtg atttcagtca taaaaaaacc
      601 cgcctggcga
//
LOCUS       DAZFYC010000459          609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFYC010000459 DAZFYC010000000
VERSION     DAZFYC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.466"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..576
                     /locus_tag="ACHKW5_005732"
     CDS             <1..576
                     /locus_tag="ACHKW5_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7514845.1"
                     /translation="SGNPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRV
                     LGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFS
                     GAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVS
                     AWLVNNWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          157 a          146 c          166 g          140 t
ORIGIN      
        1 tccggcaatc ccccgacgtt ttccctgacg ccggagggca ggctgaccgc aagaaatgct
       61 gatatcagcg gtaacgtgaa tgcgaattcc gggacgctca acaacgtcac gattaacgag
      121 aactgtcggg ttctgggaaa actgtccgcg aaccagattg aaggcgatct cgttaaaaca
      181 gtgggcaaag ctttcccccg ggattcccgt gcaccggagc ggtggccatc agggaccatt
      241 accgtcaggg tttatgacga tcagccgttt gaccggcaaa ttgttattcc ggcggtggca
      301 ttcagcggcg ctaaacatga gagagagcat actgatattt actcctcatg ccgtctgata
      361 gtgcggaaaa acggtgctga aatttataac cgtaccgcgc tggataatac gctgatttac
      421 agtggcgtta ttgatatgcc tgccggtcac ggtcacatga cgctggagtt ttcggtgtca
      481 gcatggctgg taaataactg gtatcccaca gcaagtatca gcgatctgct ggttgtggtg
      541 atgaaaaaat ccacagcagg tatcagtatc agctgaattt tataacccag aacgggcgtc
      601 agaaatgac
//
LOCUS       DAZFYC010000460          581 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFYC010000460 DAZFYC010000000
VERSION     DAZFYC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.467"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..206)
                     /gene="ydfK"
                     /locus_tag="ACHKW5_005733"
     CDS             complement(<1..206)
                     /gene="ydfK"
                     /locus_tag="ACHKW5_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO7514846.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLK"
     gene            complement(275..388)
                     /locus_tag="ACHKW5_005734"
     CDS             complement(275..388)
                     /locus_tag="ACHKW5_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO7514847.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT          158 a           96 c          117 g          210 t
ORIGIN      
        1 tctttaagaa ctgatatcgc tgtttgtaat aattctttgt tatccagcca tgactttttc
       61 tttatgtttc cttcaatgta atcaagcaat gttctggtat tgataggtct tccctgtttt
      121 gctacttcca ctacagcatc ccctaggata attcttactt caggaagctg cgcagggaac
      181 cactttaggg tgtcttttga tttcatgaag atattcctta aaatattatt gattttcatt
      241 gcgatattgt atgtctgatt caggatatgt tgacttatac atcggttttg tctgggttat
      301 tggatatgcc aatccctaat tttattagag catgactaaa aatgctgaat atgataagga
      361 gcgaagtgat tatcagtatg ctgttcatat agcctcgaat tagtaatgtg ttatatatga
      421 tatagttgac aatttttatc ctgggtgttc ttaaagttcg tagataaaca ttgtcgtttc
      481 aggtatacag gaatgctaac aggtggcggc aaaaatcagg cggtttatgg cgcaagctga
      541 agtggcaact gcaaactatc ttatgcagag actctacacg g
//
LOCUS       DAZFYC010000461          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFYC010000461 DAZFYC010000000
VERSION     DAZFYC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.468"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..424
                     /locus_tag="ACHKW5_005735"
     CDS             215..424
                     /locus_tag="ACHKW5_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514848.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(425..>580)
                     /locus_tag="ACHKW5_005736"
     CDS             complement(425..>580)
                     /locus_tag="ACHKW5_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514849.1"
                     /translation="RWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEH
                     GPLWIDW"
BASE COUNT          173 a          134 c          120 g          153 t
ORIGIN      
        1 gccacaaccg gaagcgcacg ttcgaagaaa tctaacgaca agccttctaa gggaaagagc
       61 ttcgccgtac gctttcgcgt tatgccctga cttttcaggg aaatatcctt tcagtaaact
      121 gtcagtaccg gattcttatc cgtgtccggc gcacgaccac acgtgacagc gtgttggtct
      181 ccatttttaa cccagaacct caatggagga taaaatgcca aacaaaaaaa gaaatccgct
      241 tattgaaaaa cagattgaat gcctggtaaa tcaactcagg caatcagggt tattaaaaac
      301 tcattcagag ttgaggctca cagaatcagc attcgacgat aaattaaata atgtccttta
      361 taatggcatt attgatttta atcgttctgt tggtcgccgc ggccctgctg gtgtttcctt
      421 ataattacca gtcaatccag agtggaccgt gttcagcgta aatataactg tacacatcca
      481 gattatattt gtggtctgtt aagaacaggc cgcaaataca tgccgaagct tccagtgcag
      541 cggctctgtt actgaataac catgtagcaa cattccagcg
//
LOCUS       DAZFYC010000462          579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFYC010000462 DAZFYC010000000
VERSION     DAZFYC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.469"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>579
                     /locus_tag="ACHKW5_005737"
     CDS             <1..>579
                     /locus_tag="ACHKW5_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO7514850.1"
                     /translation="LSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANE"
BASE COUNT          156 a          138 c          141 g          144 t
ORIGIN      
        1 ctttccactg ccagcatcga tgattttacc cgttattcta aagatcttgc agatgaaggc
       61 acccgctgct ttatcgatgc cgataatatg cgtgccgtca gtgtgcttaa cctgggtact
      121 attgatgagc caggtcacgc agataacact gccactctca aactgaaaaa gacagcaccg
      181 ttctctgctc tgttgtctgt taacggcgag cgtaactccc agaagtcact ggcagaatgg
      241 atcgaagact gggccgacta ccttgtgggc tttgatgcta atggtgacgc cattcaagca
      301 acaaaagcgg ctgcggcagt ccgtaaaatc acgattgaag caaaccagac cgctgatttt
      361 gaagataatg acttcagcgg caaacgctcc ctgatggagt ctgtcgaagc gaagaccaaa
      421 gacattatgc cagtggcatt tgaatttaaa tgcgttccgt ttgaaggtct gaaagaacgt
      481 ccgtttaaat tacgcctcag cattatcact ggcgatcgtc ctgtactggt tctgcgcatt
      541 attcagctgg aagcggtgca ggaagaaatg gctaacgaa
//
LOCUS       DAZFYC010000463          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFYC010000463 DAZFYC010000000
VERSION     DAZFYC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.470"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..514
                     /locus_tag="ACHKW5_005738"
                     /pseudo
     CDS             1..514
                     /locus_tag="ACHKW5_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            495..>576
                     /locus_tag="ACHKW5_005739"
     CDS             495..>576
                     /locus_tag="ACHKW5_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514851.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVI"
BASE COUNT          192 a           80 c          136 g          168 t
ORIGIN      
        1 caggacccga tagggctgga gggggggatg gagtctgtat gcgtacccgc tgaatccggt
       61 gaatggtatt gatccattag ggttaagtcc cgcagatgta gcgctaataa gaagaaaaga
      121 tcaactaaac catcaaagag catgggatat attatctgat acttatgaag atatgaagag
      181 attaaattta ggtgggactg atcaattttt ccattgtatg gcattttgtc gagtgtctaa
      241 attaaatgac gctggtgtta gccgatcggc gaaagggctg ggttatgaaa aagagattag
      301 agattacggg ttaaatctgt tcggtatgta cggcagaaaa gtaaagctat cccattctga
      361 aatgattgaa gataataaaa aagacttggc tgtaaatgac catgggttga catgtccatc
      421 aacaacagat tgctcagata gatgtagtga ttatattaat ccagagcata aaaaaacgat
      481 aaaggcttta caagatgctg gctatctcaa gtaatctatc aaagatgata atatttattt
      541 ttgctattat aatcattgtt gttttatgcg taatta
//
LOCUS       DAZFYC010000464          569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFYC010000464 DAZFYC010000000
VERSION     DAZFYC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.471"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..293
                     /locus_tag="ACHKW5_005740"
     CDS             <1..293
                     /locus_tag="ACHKW5_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7514852.1"
                     /translation="AGGFSATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQN
                     TYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
BASE COUNT          154 a          123 c          177 g          115 t
ORIGIN      
        1 cagccggtgg tttttctgcc accacgatgc tgttttcact gggtgccagc atgatactgg
       61 gtggtgtggc acagatgctg gccccgaagc caaaaacacc ggaatacagg gcaacggata
      121 acggtaaaca gaacacgtac ttttcgtcgc tggataacat gattgcccag gggaacccga
      181 tgccggtgcc ttacggtgaa atgctggttg gctcacggcg aatctcccag gacatcagta
      241 cccgtgatga aggcggtgac gggaaggtgg tggttatcgg gcggcagggg taaagcataa
      301 aaaaatcccg cagtgtatgg aggctgcggg aacagaaaat gaagattaac cacagggagt
      361 tttgttttta ttggcccgaa aaaactgtaa cgcccgggaa tgatatctgc cacgggggcg
      421 tacagaaaat gtgaagaaat tcagaaattt tattccgtca tgacacaggc accctccggg
      481 gtgcctgtcg tttttggggc ataaacagat tcagacatca gacaggagag ggggacagag
      541 tgggtaaagg tggcggcaag gcgcacacg
//
LOCUS       DAZFYC010000465          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFYC010000465 DAZFYC010000000
VERSION     DAZFYC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.472"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>567
                     /locus_tag="ACHKW5_005741"
     CDS             <1..>567
                     /locus_tag="ACHKW5_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7514853.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRYILHRGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVR"
BASE COUNT          108 a          167 c          178 g          114 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcggcgctt ccgtctgagt ggccacggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacagg gatgcagagt atgaagccct gtaccgtgac
      181 attctgcccg aactggacct ggtgctgtgg ctgattaaag ccgatgaccg cgccctgtct
      241 gtggatgagt atttctggcg atacatcctg caccggggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg cattcagcct
      361 tctcccgcac aggcacagaa cattcgggag aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
      541 gacgagctgc gcacggaatc tgtccgc
//
LOCUS       DAZFYC010000466          551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFYC010000466 DAZFYC010000000
VERSION     DAZFYC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.473"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            272..385
                     /locus_tag="ACHKW5_005742"
     CDS             272..385
                     /locus_tag="ACHKW5_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514854.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            387..>551
                     /locus_tag="ACHKW5_005743"
     CDS             387..>551
                     /locus_tag="ACHKW5_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7514855.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDHAIIYKEQRDKKASEL
                     ELANATITDMQ"
BASE COUNT          142 a          106 c          162 g          141 t
ORIGIN      
        1 tcacggcgtg accaggagag cgcgctggcg tgctgggaca tcgacagata gcagaatatt
       61 ttcctgaaaa atgacgttgg ccaacgcggg tggataacac gaaatcctga aaactggtaa
      121 aacctaagtg aataaaagta aaaaccccgt ttgttggcag caagcggggt tttgtgtttt
      181 ctgaccttga gtaaggcaag ggagaaatta tgggtaggga ggtactttcc ctgtgaggaa
      241 gtataaaaga ttctttctga ggttgtccat tatgaaaggc attgaagtgg agacgccagc
      301 cagtctggat ttaacaagag cggcagcttt tgccattcgt attgtggcca ttgctgttct
      361 ggtctgggca atccgttggt ggtgatatga accgtgttct gtgcgtggtt atcattgtcc
      421 tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca ttaccgtgat cacgccatca
      481 tctacaaaga gcagcgcgat aaaaaagcca gtgagctgga gctggcgaac gcgacaatta
      541 ctgatatgca g
//
LOCUS       DAZFYC010000467          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFYC010000467 DAZFYC010000000
VERSION     DAZFYC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.474"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(157..232)
                     /locus_tag="ACHKW5_005744"
     tRNA            complement(157..232)
                     /locus_tag="ACHKW5_005744"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
     gene            complement(261..>549)
                     /locus_tag="ACHKW5_005745"
     CDS             complement(261..>549)
                     /locus_tag="ACHKW5_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7514856.1"
                     /translation="TDISASWTLGAIALQLHMSESLLKTKLKNEGGMFSRLLLEERMR
                     VAVNMLCSRHGYGQAIAEKCGYSSRSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          152 a          147 c          115 g          135 t
ORIGIN      
        1 aaccactgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa caacgcagaa
       61 taccgataat ggaccgccat cggggacccg gccccgcacc aacaaccctg ttatggtgtc
      121 gtctgctctt cctgataagc taatggcggt ttgtgatggt ggcccttgct ggatttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgagccaaa
      241 aaataataat cagatgaaaa tcaataatca agcccttgcc tggatacata tctgtctggc
      301 gggaagccat aatagcggtg aaatacagaa ataaagtagg acctgcttga ataaccgcat
      361 ttttctgcta tagcctgtcc atatccatgc cgggaacata acatatttac agcaacacgc
      421 atccgctctt ccagcaacaa gcgactgaac atgccccctt catttttcag ttttgtcttt
      481 aacaaactct cactcatatg caactgtaga gcaatcgcac caagcgtcca gcttgctgat
      541 atatctgtc
//
LOCUS       DAZFYC010000468          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFYC010000468 DAZFYC010000000
VERSION     DAZFYC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.475"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>549)
                     /locus_tag="ACHKW5_005746"
     CDS             complement(<1..>549)
                     /locus_tag="ACHKW5_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO7514857.1"
                     /translation="SGDKSDIIYKVNGLSIIPENFFTLLSGNFKADWRWDVSKETFPK
                     DKFDSYVKSVFSKIDFYKHCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREK
                     GWSLLCELLKHENMASSAYIHKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFL
                     GDHTGDRFSTIFGDKYILTLLNS"
BASE COUNT          186 a          104 c           85 g          174 t
ORIGIN      
        1 agaatttagc aaagtaagga tatatttatc accaaaaata gtactaaatc tatcgcctgt
       61 atgatcccca aggaagatac aaggggttat cgcatcgtcc tttaaaaaaa catgttgatg
      121 caactcattc aatatcatgt ctctcgtatt taataaattg tagagcaatt tattattttt
      181 atgtatgtat gcagatgaag ccatgttttc gtgttttaat aattcacata ataaactcca
      241 acccttttcc ctgatgccta tatgccctga aacgagcaat gcataaagta cagcgcctac
      301 tcttccatct gtatcgccaa aataagccgt gttggcattt tgtgggttaa tcacaccaca
      361 gtgtttataa aagtctattt tagaaaacac agatttaaca tagctatcaa acttatcctt
      421 aggaaacgtc tctttagaga catcccaacg ccagtcagct ttaaaattac cgctaagaag
      481 agtaaagaaa ttttcaggta tgatagaaag gccattaact ttataaatta tgtctgattt
      541 atctccgga
//
LOCUS       DAZFYC010000469          547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFYC010000469 DAZFYC010000000
VERSION     DAZFYC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.476"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..212
                     /locus_tag="ACHKW5_005747"
     CDS             <1..212
                     /locus_tag="ACHKW5_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7514858.1"
                     /translation="LAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTD
                     ITRNATYPVLIDSIAVEVNSTDTAA"
     gene            348..527
                     /locus_tag="ACHKW5_005748"
     CDS             348..527
                     /locus_tag="ACHKW5_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7514859.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
BASE COUNT          149 a          123 c          149 g          126 t
ORIGIN      
        1 cgctggcttt ccgctttgcc gggaataaca gccttcaggt gacgccggtt attgatggtc
       61 aggatggcac accgttcacg ctgacgcagt caccggtcag tgcatttgcg gcggataaac
      121 tgcatgtgac agacattacc aggaatgcga cttacccggt actgatagac agcattgcgg
      181 tggaagtgaa cagcacagac actgcggcat gataaaaaaa ccgccagcga caggaatgga
      241 cgctggcggt ggtgatacct atggagaaaa aataaaggaa cgatactttc gtactctggt
      301 tttttaatga aaacagttct tattgtcaac aataacggaa agaaattatg acatttctga
      361 accagttaat gctgtacttc tgtacggtgg tctgtgtgct gtatctcctt tcgggtgggt
      421 acagggcagt gcgcgattgc tggcgcaggc agattgacaa aagggccgct gagaaaatca
      481 gcgccggtca gtcagccgga agcaaacccg aagagccgct catttagcgg caactttctt
      541 aatcaca
//
LOCUS       DAZFYC010000470          546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFYC010000470 DAZFYC010000000
VERSION     DAZFYC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.477"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..176)
                     /locus_tag="ACHKW5_005749"
     CDS             complement(<1..176)
                     /locus_tag="ACHKW5_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7514860.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVV"
     gene            complement(234..>546)
                     /locus_tag="ACHKW5_005750"
     CDS             complement(234..>546)
                     /locus_tag="ACHKW5_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7514861.1"
                     /translation="VRIFAGNDPAHTATGSSGISSATPALTPLMLDEASGKLVVWDGQ
                     KAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKANAFAGSALSHAAL
                     P"
BASE COUNT          122 a          160 c          135 g          129 t
ORIGIN      
        1 tcaacaaccg gggagacata ggccgccacc ggcgtttttc cggtaatttt gtccagcatc
       61 acctcttcgg tatggaaatt caccgtacgg cggaaaaaca gctccagaaa cagcgcacga
      121 aatttcactt tttgttcggt ataaccgagt aactggcggg tcgtaaacaa tcccataaat
      181 cagttccttt cattcagaaa tcagtcaggc caccgcggtg gcctgataac gtgttacggc
      241 agcgccgcgt gactcagggc actgccggca aaggcatttg cctttttgtg ttcatccaca
      301 ctttcaggcc agcggattgc ctccgtcgca aaggtccccg acttgtaata ggtcagtacc
      361 gtctctgtgc cttcaagcgg cagtaccagt atgccaactg cactaccggc tttctgtccg
      421 tcccagacca ccagtttccc gctggcttca tccagcatca ggggcgtcag tgccggtgtt
      481 gccgaggaaa tcccgctgct gcctgtggcg gtatgagccg gatcattacc ggcaaaaata
      541 cgtact
//
LOCUS       DAZFYC010000471          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFYC010000471 DAZFYC010000000
VERSION     DAZFYC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.478"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            297..>545
                     /locus_tag="ACHKW5_005751"
     CDS             297..>545
                     /locus_tag="ACHKW5_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514862.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIGTLVKNIFIRI"
BASE COUNT          135 a           81 c          142 g          187 t
ORIGIN      
        1 tcatgcgggc agcaatgcct gtgctgttgg gtttcatttc acctccgtgc ggttcatatg
       61 ttaagtctaa agagcgtgtt tgctgaacgg aaacgagtta tttcgatcgt tatgatcgag
      121 tgtgttccaa gagtgctttt aactctgttt taagcgtgaa tgaaaaggat tcggacagga
      181 cgtgatttag catgccgctg gtctggcatt atgctgcaga gcagacggag tcaggagatt
      241 gaaagtattt cgttatataa tcttttcctt ttcgggatga aatcaacgag gttcgtatga
      301 aaattaaaca gcataaagaa aaactggaga ttataaggga tattattcga gagacgctgt
      361 tgggtaatgc tgctcttatt gttctggcag ccatggttcc ggttgtactg gtgaaaccat
      421 gggattcgat gacatttatt tttatggggg ccgtgtgtgt ttcttttgca gtcattctgt
      481 tctggctggt ttatatagtt tgtttgtata taggaacgct tgtaaagaat atttttataa
      541 ggatt
//
LOCUS       DAZFYC010000472          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFYC010000472 DAZFYC010000000
VERSION     DAZFYC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.479"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>528)
                     /locus_tag="ACHKW5_005752"
     CDS             complement(<1..>528)
                     /locus_tag="ACHKW5_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7514863.1"
                     /translation="GSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGY
                     FNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKY
                     NTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKE
                     DLLKPVNSNQYRYCAK"
BASE COUNT          162 a          108 c           83 g          175 t
ORIGIN      
        1 ttttgcacaa taccgatact gattgctatt tacaggttta agtaaatctt ctttactcca
       61 cggagaagct gctttggcga tcaaaaaact acgtgacacc ggatagtcag cccactcatg
      121 ccagggtttt gtaggtccca catagtgtat aaacacggtg tcatcattga ctggattatc
      181 aactttgtct tttaattcat agttaatact atatcgggta ttatatttct ccgaaataaa
      241 cttcacttta ccattcaata atacattcag tacatcttga tcaaggtggg tgattttact
      301 tacccaatct gggtcacgca acatttcaat agcttttgac gaaatgttat ttagattcca
      361 ctcatcaata tttatcagta aaaaaccagc attgaaatat ccagaagcta actgtggtgt
      421 agttaatacc gaggctcgat tttgccacca ttcaacatct ctttcagcta caactgcggc
      481 aatttcatta gtagaaaatt gataatctaa gagttcttta atactacc
//
LOCUS       DAZFYC010000473          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFYC010000473 DAZFYC010000000
VERSION     DAZFYC010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.480"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..95)
                     /gene="yrhD"
                     /locus_tag="ACHKW5_005753"
     CDS             complement(<1..95)
                     /gene="yrhD"
                     /locus_tag="ACHKW5_005753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO7514864.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYD"
     gene            complement(190..>521)
                     /gene="yrhA"
                     /locus_tag="ACHKW5_005754"
     CDS             complement(190..>521)
                     /gene="yrhA"
                     /locus_tag="ACHKW5_005754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFO7514865.1"
                     /translation="LFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEF
                     YRNNDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLN
                     EIMDSCS"
BASE COUNT          172 a           86 c           81 g          182 t
ORIGIN      
        1 tcatcatagc cattagctat ttcggctaaa atagagacta catgtcttcg gtccatctca
       61 cttaaggagt gtagttccgt tgtaagtttt tccatagctt gcactgctaa atttcgaaca
      121 aggaattttc tgctggtaat ctctaaaaag atggcatggt ttacaatgat ttttgtttcc
      181 ttttgattat tatgaacaac tgtccatgat ttcgtttaag aatgaagaga aatcactaaa
      241 cgaactgaat atattttctg tgccaatatt atctctaatt tcaaaaaagt tacttttaat
      301 gtcataagta aatattgaaa tgctatcatc cccgatcact aaccgttctt gtagatcagg
      361 gtttatgaaa tcatcattat ttctataaaa ttcatttacg gcaaaaaggt ttttaactga
      421 aggttctgga atcgagaggc taaataatcg taatccatta acttcgaatc catccattgc
      481 tcttaaaaaa gtaagataat caggttgatt cttaaaaaga a
//
LOCUS       DAZFYC010000474          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFYC010000474 DAZFYC010000000
VERSION     DAZFYC010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.481"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>520
                     /locus_tag="ACHKW5_005755"
     CDS             <1..>520
                     /locus_tag="ACHKW5_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514866.1"
                     /translation="EKRTYSGIWDGTLKPAYSNNIAWCLWDMLTHPRYGMGKRLGAAD
                     VDKWALYVIGQYCDQSVPDGSGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWN
                     GQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWE
                     TATELVEDTQAIA"
BASE COUNT          115 a          132 c          172 g          101 t
ORIGIN      
        1 cggaaaaacg cacatacagc ggtatctggg acggaacgct taaaccggca tacagcaaca
       61 acatagcctg gtgtctgtgg gatatgctga cccatccgcg ttatggcatg gggaaacgtc
      121 ttggtgcagc ggatgtggat aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt
      181 cggtgccgga cggctctggt ggcacggagc cgcgcatcac ctgtaatgcg tacctgacca
      241 cacagcgcaa ggcgtgggat gtgctcagtg atttctgctc ggcgatgcgc tgtatgccgg
      301 tatggaacgg gcagacgctg acgttcgtgc aggaccgacc gtcggataag gtgtggacct
      361 ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg
      421 ccctgaagga ccgccataat gccgttgagg tgaactggat tgacccgaat aacggctggg
      481 agacggcgac agagcttgtg gaggacacgc aggccattgc
//
LOCUS       DAZFYC010000475          512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFYC010000475 DAZFYC010000000
VERSION     DAZFYC010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.482"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>512)
                     /locus_tag="ACHKW5_005756"
     CDS             complement(<1..>512)
                     /locus_tag="ACHKW5_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7514867.1"
                     /translation="FTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRRRRIIMQNM
                     RDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEENNITQSGGTEWSKRD
                     KSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGSNSELETAV
                     KDLGKAVSYKG"
BASE COUNT           97 a          159 c          132 g          124 t
ORIGIN      
        1 cccttatagg acaccgcttt acctaggtct ttcaccgctg tctccagctc ggaattagag
       61 ccacgacggg tatccagctt ctccttgacg gctttgaagg aacggaacag cgcccagcct
      121 ttcggatcga acacgatgat attcaccaca ccgctggcgt tcagcgcgta ggcttcgata
      181 tcgtcggtcg ggtcatacgt ggacttgtca cgcttgctcc actccgtgcc gccggactgc
      241 gtgatgttat tctcctcact gcggcccata tccacctcaa ccggatcgaa ggcttcaccg
      301 gtcatggtgt atttgccctt aagcacggca gaaactgcct gcatctcttc gacctgagca
      361 atggccagct cttcgtcacg catgttctgc atgatgatgc gacggcggcg gtaagccggg
      421 tccgccagat tctgcggatc ttcatccggc aggcgacgca gggtcatctg cggattcact
      481 tcatgcttcg gcttgacata tcccggcgta aa
//
LOCUS       DAZFYC010000476          512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFYC010000476 DAZFYC010000000
VERSION     DAZFYC010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.483"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            273..>512
                     /locus_tag="ACHKW5_005757"
     CDS             273..>512
                     /locus_tag="ACHKW5_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7514868.1"
                     /translation="MKTLSQNTTSSVSAPETDLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAF"
BASE COUNT          106 a          147 c          125 g          134 t
ORIGIN      
        1 gggtgtcgtt ccatttctga tgatgacctg tgcgcctggt gtacccgtct gctttaccgt
       61 cccggagaac tcagtctgtg cagactgagt acaggcaatg gcatctggcc atccgtctgt
      121 gaccggaacg gttagcgtac ggctgtccgg aatttcagcc gaatatcatt catccctgac
      181 attaccgggt ctcacggtca gtgatgaacg tgatgttgtc agcccccggc ctttactccc
      241 tctcatttat tcacattaca aggatttcat acatgaaaac attgtctcag aacaccacct
      301 cttcggtctc agcaccggag actgacctgc aacaactggt tgccaccata gtccctgatg
      361 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtgaccctgg
      421 agccccgtat cttcggctgg atggaccgtc tttgcgaaga ctactgcggt ggtatctgga
      481 atctgtacac cctgaacaac ggtggcgcat tt
//
LOCUS       DAZFYC010000477          504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFYC010000477 DAZFYC010000000
VERSION     DAZFYC010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.484"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..302
                     /locus_tag="ACHKW5_005758"
     CDS             <1..302
                     /locus_tag="ACHKW5_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO7514869.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>504
                     /locus_tag="ACHKW5_005759"
     CDS             299..>504
                     /locus_tag="ACHKW5_005759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO7514870.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALW"
BASE COUNT          114 a          117 c          169 g          104 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtacacag atcagcatgg tttcagggac
      421 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
      481 cgtacgcctg gaggcgctct ggga
//
LOCUS       DAZFYC010000478          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFYC010000478 DAZFYC010000000
VERSION     DAZFYC010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.485"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>499
                     /locus_tag="ACHKW5_005760"
     CDS             <1..>499
                     /locus_tag="ACHKW5_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7514871.1"
                     /translation="LEYKRQLAALGDKVTYQERLNALAQQADKFAQQQRAKRAAIDAK
                     SRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLRGNWMAGLKSGW
                     SEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQ
                     AMVGLS"
BASE COUNT          131 a          122 c          168 g           78 t
ORIGIN      
        1 ctggagtaca aacgccagct ggctgcactt ggcgacaagg ttacgtatca ggagcgcctg
       61 aacgcgctgg cgcagcaggc ggataaattc gcacagcagc aacgggcaaa acgggccgcc
      121 attgatgcga aaagccgggg gctgactgac cggcaggcag aacgggaagc cacggaacag
      181 cgcctgaagg aacagtatgg cgataatccg ctggcgctga ataacgtcat gtcagagcag
      241 aaaaagacct gggcggctga agaccagctt cgcgggaact ggatggcagg cctgaagtcc
      301 ggctggagtg agtgggaaga gagcgccacg gacagtatgt cgcaggtaaa aagtgcagcc
      361 acgcagacct ttgacggtat tgcacagaat atggcggcga tgctgaccgg cagtgagcag
      421 aactggcgca gcttcacccg ttccgtgctg tccatgatga cagaaattct gcttaagcag
      481 gcaatggtgg gattgtcgg
//
LOCUS       DAZFYC010000479          499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFYC010000479 DAZFYC010000000
VERSION     DAZFYC010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.486"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            64..472
                     /locus_tag="ACHKW5_005761"
                     /pseudo
     CDS             64..472
                     /locus_tag="ACHKW5_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          153 a          107 c           98 g          141 t
ORIGIN      
        1 tatatctatc tgtcattttg caaatgatat gcggtagctt tacctaacta tcatgaacat
       61 ttcgaaaaat catccacaaa gccccgaaga ataaagcact atcgcccgat aaagacctgt
      121 aagctcagtg gcatggatac tattgagctc agaatggcga gctgtaccgt tatttcatta
      181 ccatgctcga cgaacacagc aactactctc tggccgtact atcgcttaaa agtgatatca
      241 tcaggcattt cttctgctgt gtagcccagt tgttccttgt tggtatcaac cagatcatca
      301 ctgatgatgg gcaaagagtt ctcgggaaaa ttcgacaaaa cgctacagga agccaccgtc
      361 gggttcgtat ggacttatct ctatacgccg aaaattaaat gccataagtg agcattttaa
      421 ccgaacactg agggaacaat ttattgagtt taatgaagaa ttgctctttg aatagctgaa
      481 tatgttcaat ctgaaactg
//
LOCUS       DAZFYC010000480          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFYC010000480 DAZFYC010000000
VERSION     DAZFYC010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.487"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..189
                     /locus_tag="ACHKW5_005762"
     CDS             <1..189
                     /locus_tag="ACHKW5_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7514872.1"
                     /translation="DSRSQASGTFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAM
                     DVVTGQMRDGGLFSGGGR"
     gene            186..>498
                     /locus_tag="ACHKW5_005763"
     CDS             186..>498
                     /locus_tag="ACHKW5_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514873.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGCSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EF"
BASE COUNT          104 a          114 c          172 g          108 t
ORIGIN      
        1 gacagtcggt cgcaggcgtc cgggacgttt gagcagaata accatgtggt gattaacaac
       61 gacggcacga acggtcagat agggccacag gcgctgaagg ctgtttatga cgtagcccgt
      121 aaggcggcaa tggatgttgt gaccgggcag atgcgtgatg gtggtctgtt ctccggaggt
      181 ggacgatgaa aaccttccgc tggaaagtga aacccggtat ggatgtggct tcggcccctt
      241 ctgtaagaaa ggtgcgcttt ggtgatggct gttcccagcg agcgcctgcc gggctgaatg
      301 ccaacctgaa aacgtacagc gtgacgcttt ctgtcccccg tgaggaggcc acggtactgg
      361 agtcgtttct ggaagagcac gggggctgga aagcctttct gtggacgccg ccttatgagt
      421 ggcggcagat aaaggtgacc tgcgcaaaat ggtcgtcgcg ggtcagtatg ctgcgtgttg
      481 agttcagcgc agagtttg
//
LOCUS       DAZFYC010000481          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFYC010000481 DAZFYC010000000
VERSION     DAZFYC010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.488"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..497
                     /locus_tag="ACHKW5_005764"
     rRNA            <1..497
                     /locus_tag="ACHKW5_005764"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          122 c          148 g          106 t
ORIGIN      
        1 caggtgctgc atggctgtcg tcagctcgtg ttgtgaaatg ttgggttaag tcccgcaacg
       61 agcgcaaccc ttatcctttg ttgccagcgg tccggccggg aactcaaagg agactgccag
      121 tgataaactg gaggaaggtg gggatgacgt caagtcatca tggcccttac gaccagggct
      181 acacacgtgc tacaatggcg catacaaaga gaagcgacct cgcgagagca agcggacctc
      241 ataaagtgcg tcgtagtccg gattggagtc tgcaactcga ctccatgaag tcggaatcgc
      301 tagtaatcgt ggatcagaat gccacggtga atacgttccc gggccttgta cacaccgccc
      361 gtcacaccat gggagtgggt tgcaaaagaa gtaggtagct taaccttcgg gagggcgctt
      421 accactttgt gattcatgac tggggtgaag tcgtaacaag gtaaccgtag gggaacctgc
      481 ggttggatca cctcctt
//
LOCUS       DAZFYC010000482          495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFYC010000482 DAZFYC010000000
VERSION     DAZFYC010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.489"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>495
                     /locus_tag="ACHKW5_005765"
     CDS             <1..>495
                     /locus_tag="ACHKW5_005765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO7514874.1"
                     /translation="YALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDER
                     LKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATT
                     NSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAM
                     GERMT"
BASE COUNT          139 a          119 c          136 g          101 t
ORIGIN      
        1 atacgcgtta atcgaacaat ggcgacaaca atttcccatt gaagcgatgt gtcaggtatt
       61 tggtgtatcc aggagcggtt attacaactg ggtacagcat gaaccctcag acagaaaaca
      121 aagtgatgag cggctaaaac tggagattaa ggtggcacat atccgcactc gcgaaacata
      181 tggaacccgg cggctccaga cggagctggc agagaatggc atcatcgttg gtcgtgaccg
      241 actggcacgt cttcgtaagg agctaaggct acgctgtaag cagaaacgca agttcagagc
      301 gactacgaac tcgaaccaca atctgccagt tgcgccaaat ctgctgaacc agacgttcgc
      361 tcctacagca ccaaatcagg tctgggtggc ggacctgacg tatgttgcca cacaggaggg
      421 atggttgtac ctcgctggca tcaaagatgt ttatacgtgc gaaattgtcg gctacgccat
      481 gggagagcgc atgac
//
LOCUS       DAZFYC010000483          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFYC010000483 DAZFYC010000000
VERSION     DAZFYC010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.490"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            179..494
                     /locus_tag="ACHKW5_005766"
                     /pseudo
     CDS             179..494
                     /locus_tag="ACHKW5_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          176 a           77 c           76 g          165 t
ORIGIN      
        1 ttttacaatt atcctccagg gatggattgt tttccattat ttagtaaaag accggatagt
       61 ctcggttctt aaaatcatcg aacatataag tattattgct ttgtatcatc gtaaatatgt
      121 ctttcagaca tgtattgttt attttgttac taacaagtaa aaatattaca ggttatttat
      181 gaaagtattt cacaatatat ttaagcactt ctcttcaaac catcaagata agcattcaga
      241 taaagttaac agtcatcaac accatggcaa ggttgataaa aaatatcgat caaacacagt
      301 ttaagttaat atgctgaatg acttttcgca aatagataac gatttaggcc tgaatatcat
      361 ctatttcctg caacatggtc actggagggt aaatgaacat agtcttcaga tggagaaaat
      421 atggttttac aatagtgagc catctataga tattcaagaa tataatagtt tcgccaagaa
      481 tactactaac acgt
//
LOCUS       DAZFYC010000484          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFYC010000484 DAZFYC010000000
VERSION     DAZFYC010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.491"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>491)
                     /locus_tag="ACHKW5_005767"
     CDS             complement(<1..>491)
                     /locus_tag="ACHKW5_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7514875.1"
                     /translation="ALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQ
                     TFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGG
                     ASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYR
                     LMRG"
BASE COUNT          100 a          182 c          113 g           96 t
ORIGIN      
        1 ccgcgcatca gacggtaaag attccccaca cctatccggc tggttgcctc tttcgtgaaa
       61 acaaactccc cgcggtgaac tatccccgca ggctcatatt tgccgcccgt ccccgtaaat
      121 cctccggtcg cgaaatggaa gttcgccgcc gcagcctcaa tggccgtccc cgaggaagca
      181 gatgcaccac caccgaaagc accgccaatg gcgctgccga tacgcccgac aatgcccacc
      241 atggcctgtt taagcaggat ttctgtcatc atggacagca ccgaacgggt gaatccccgc
      301 cagtctgcct ctgcaccggt cagcatcgcc gccatattct gtgcaatacc gtcaaaggtc
      361 tgcgtggccg cgcttttaac ctgcgaaaaa ctgtccgtcg cactttccgc ccactccccc
      421 cagccggact tcagcccggc cagccagtca ccgcgcagca tgtcttcatc cgcccatgtc
      481 tgtttcagtg c
//
LOCUS       DAZFYC010000485          486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFYC010000485 DAZFYC010000000
VERSION     DAZFYC010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.492"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..341)
                     /locus_tag="ACHKW5_005768"
     CDS             complement(<1..341)
                     /locus_tag="ACHKW5_005768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514876.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRV"
BASE COUNT           94 a          151 c          113 g          128 t
ORIGIN      
        1 acccgcaggc ggtcaatgtt cgcggatgta atggtgcgcg ttaccggctt tgccttcgtc
       61 acttccacgc ccagtgcggt ttccgccccg gaggactcaa agccttcagg tggtgtctgc
      121 tcctgctccc cggcgcgcca gaccgcggtc acaccatgta tcacaggatt accgtccgtg
      181 tccgtcagcg gggttttgtt caccaggata ctctgcagcc ccttcaccgg accttcaatc
      241 ggcccttcac caatggcgtc aatcacgctc atcatctgcg tggatttgag attgtccttc
      301 gcctcgcgcg gtgtgtgcgc cttgccgcca cctttgccca ttgtctcacc ctttactgtg
      361 ataactgtta cgcacaaaaa caacaggcat cccggaggat gcctgtatca tgactgaata
      421 aaaattctga atatcttcac attttcacaa actgactgtg gcgctaataa tttctctgcg
      481 ttaatg
//
LOCUS       DAZFYC010000486          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFYC010000486 DAZFYC010000000
VERSION     DAZFYC010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.493"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..485)
                     /locus_tag="ACHKW5_005769"
     CDS             complement(<1..485)
                     /locus_tag="ACHKW5_005769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7514877.1"
                     /translation="MRVFHGGRVLVESEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     NERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVS
                     RR"
BASE COUNT          119 a          140 c          120 g          106 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acagtttcgc tgtgatgata atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcat
//
LOCUS       DAZFYC010000487          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFYC010000487 DAZFYC010000000
VERSION     DAZFYC010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.494"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..275)
                     /locus_tag="ACHKW5_005770"
     CDS             complement(<1..275)
                     /locus_tag="ACHKW5_005770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO7514878.1"
                     /translation="MATPNPLEPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEG"
     gene            complement(283..>483)
                     /gene="gpU"
                     /locus_tag="ACHKW5_005771"
     CDS             complement(283..>483)
                     /gene="gpU"
                     /locus_tag="ACHKW5_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO7514879.1"
                     /translation="VFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTMVTQGYEY
                     RRDDDMALWSSADLTYSITYEM"
BASE COUNT          101 a          152 c          109 g          121 t
ORIGIN      
        1 ccttcctctc ccggtttcca ggccagcgta aaactggtat cacctgcaga tttctgcccc
       61 tgcccggtcg cggtccagtc cgcgtcttca tcatccaggt agttatcatc gtaggattct
      121 gccgtcatct cgcccggcgt cagatccttc accttagcca gtcgctgcca gtcatcgtct
      181 gacaacgggt ttgcataagc atcacccttg ccggtgtaaa cccacagagt ggtaccggca
      241 ccttttaccg gctcaagggg atttggtgtt gccatatcgt cctcacatct cgtatgtaat
      301 ggaataagtc aaatctgcag agctccataa cgccatatcg tcatcacgac gatactcata
      361 gccctgcgta accatcgtgg taatcagtcc tgccagtgcc gggatcgcgg tcatcgccgg
      421 gtaaatccgg ctttccatcc actgatcaag ctctgaatcc ggtacctgtg ccggtaaaaa
      481 cac
//
LOCUS       DAZFYC010000488          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFYC010000488 DAZFYC010000000
VERSION     DAZFYC010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.495"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..275)
                     /locus_tag="ACHKW5_005772"
     CDS             complement(<1..275)
                     /locus_tag="ACHKW5_005772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO7514880.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEG"
     gene            complement(283..>483)
                     /locus_tag="ACHKW5_005773"
     CDS             complement(283..>483)
                     /locus_tag="ACHKW5_005773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7514881.1"
                     /translation="VFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTMVQQGYDY
                     RRDDDMALWSSADLTYSITYEM"
BASE COUNT          105 a          153 c          111 g          114 t
ORIGIN      
        1 ccttcttctc ccggtttcca ggccagcgta aaactggtat ctcctgcaga cttctgcccc
       61 tgcccggtcg cgctccagtc cgcgtcttca tcatccaggt agttatcatc gtaggattct
      121 gccgtcatct cgcccggcgt cagatccttc accttagcca gtcgctgcca gtcatcatct
      181 gacaacgggt ttgcataagc atcacccttg ccggtgtaaa cccacagagt ggtaccggca
      241 ccttttaccg gcgccagggg atttggtgtt ggcatatcgt cctcacatct cgtatgtaat
      301 ggaataagtc aaatctgcag agctccataa cgccatatcg tcatcacgac gatagtcata
      361 gccctgctga accatcgtgg taatcatccc tgccagtgca gggatcgcgg acatcgccgg
      421 ataaatccgg gactccatcc acgaatccag ctctgaatcc ggcacctgag caggcaggaa
      481 aac
//
LOCUS       DAZFYC010000489          469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFYC010000489 DAZFYC010000000
VERSION     DAZFYC010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.496"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            159..>469
                     /gene="grlR"
                     /locus_tag="ACHKW5_005774"
     CDS             159..>469
                     /gene="grlR"
                     /locus_tag="ACHKW5_005774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO7514882.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEKLFVDV
                     YA"
BASE COUNT          160 a           61 c          103 g          145 t
ORIGIN      
        1 atatgtcaac cacggaaaac aacaggttgg aatatgagta tatttaatga tatattacat
       61 tgcaatctgg agaaaaagaa aggtctccgt tattcttgat atcgcttatg gatagaacaa
      121 attgaaagga gtgaggttag tatgaaactg agtgagttat gattatgaag gatggcatct
      181 atagcattat ctttattagc aatgaagatt cctgtgggga aggtatactg attaaaaatg
      241 gaaatttgat cactggcgga gatattgctt ctgtgtatca gggggtcctg tctgaagagg
      301 aggacatcat acttcatgtc catcgctata atcatgaaat tccctcggtg ctaaacattg
      361 aacaagatta tcaattagtt atccctaaaa aagtactgag taatgatagt aatgtcacat
      421 tacattgcca tgtaagagga aatgaaaaat tgtttgttga tgtttatgc
//
LOCUS       DAZFYC010000490          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFYC010000490 DAZFYC010000000
VERSION     DAZFYC010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.497"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>467
                     /locus_tag="ACHKW5_005775"
     CDS             <1..>467
                     /locus_tag="ACHKW5_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514883.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRN"
BASE COUNT          103 a          117 c          130 g          117 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatat
//
LOCUS       DAZFYC010000491          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFYC010000491 DAZFYC010000000
VERSION     DAZFYC010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.498"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>467
                     /locus_tag="ACHKW5_005776"
     CDS             <1..>467
                     /locus_tag="ACHKW5_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7514884.1"
                     /translation="LWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETS
                     QRWLQTVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQA
                     AVTEAMSLFADVMNARLEQLDKAATPVEKIWDDVKTWTSDAWAWIGDHTLGAL"
BASE COUNT          119 a          100 c          158 g           90 t
ORIGIN      
        1 ttgtggaaaa tggccggggt gatcagcgat gaagccggtg gtggtatcag acaggcggca
       61 gaaaatctgg cccgtctggc agaaagcggg aaatataccg ccgggcagct acggatcatg
      121 ggggaaacct ctcagagatg gctgcagacg gtgggggacg atgccgggaa ggtggaaaaa
      181 gcctttgaag ggattgcagc agatccggtg aaggcgctgg cctccctgaa tcagcagtat
      241 aacttcctga gcgtttccca gttacgccat attgatgagc ttgagcgcac gaaaggtaaa
      301 caggctgcgg tgacggaggc gatgtccctg tttgcggatg tcatgaatgc acgtctggag
      361 caacttgata aagcggccac gccggtggaa aaaatctggg acgatgttaa aacctggact
      421 tctgacgcat gggcatggat aggtgatcat acactggggg cactcag
//
LOCUS       DAZFYC010000492          466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFYC010000492 DAZFYC010000000
VERSION     DAZFYC010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.499"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..>466
                     /locus_tag="ACHKW5_005777"
     CDS             47..>466
                     /locus_tag="ACHKW5_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO7514885.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHF"
BASE COUNT          159 a           70 c           91 g          146 t
ORIGIN      
        1 ttacactact ctaaactaga gcttatggaa ttagcgggtg gtattcatgg aaaataatga
       61 taaattctta tcacaagact tattggaatc ttatgccatt cgtttgttga gcggaccatt
      121 gaatggatgc gagtatgaaa tacttaatgg gcgtctactt gttatcattg gtaatgatgt
      181 ttcgttagga agaagtgatg ctttttctga gttaccagaa aacacaatag tcgttcctta
      241 tggcgagctt acaggtagtt ttgagataat aattactacc gaccctgatc tggtagtaac
      301 attcagagaa ttaacagcac aagaacctga agatagaacg ttaacattga atcagcaaat
      361 agaagtttta ggtctcaaat ttgctgtaaa agaaaaaaat gaagtttggc agtattcatt
      421 gccaggcatt attgaaaata acattatttc tacaaaacaa catttc
//
LOCUS       DAZFYC010000493          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFYC010000493 DAZFYC010000000
VERSION     DAZFYC010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.500"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>464
                     /locus_tag="ACHKW5_005778"
     CDS             <1..>464
                     /locus_tag="ACHKW5_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7514886.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPE
                     VSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRI"
BASE COUNT          135 a          116 c          102 g          111 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggta catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa agagaagact ctggcacttg
      121 gtgtgtatcc agaagtatct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcgga gggggttgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc tttcgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaagta ctcaaaagcc tcgagacaca cgtattcccc tttatcggca
      361 atcgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aata
//
LOCUS       DAZFYC010000494          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFYC010000494 DAZFYC010000000
VERSION     DAZFYC010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.501"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..422)
                     /locus_tag="ACHKW5_005779"
     CDS             complement(<1..422)
                     /locus_tag="ACHKW5_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514887.1"
                     /translation="MERKIIFNNGFLSINREVIIIFLIYFILVGLGISGVIYSRYVDE
                     GVKYLLVTPSFLNNSPLNWTTSVWAGVLGIHGTIAALSITFMGMFVSQVSNYSEHGFE
                     NICKSMLLRKTSFLKFSLNSIFSLLSGIVLLSCGGGMIT"
BASE COUNT          191 a           94 c           52 g          124 t
ORIGIN      
        1 gtaatcatgc cgccaccaca tgaaagcaat acgataccac ttaaaagact aaaaatagaa
       61 tttaaactaa actttaaaaa actagttttc cgcaacaaca tcgacttaca aatattttca
      121 aagccatgtt cagaataatt agaaacctga ctaacaaaca ttcccataaa tgtaattgat
      181 agtgcagcaa tagttccatg tatgcctaaa actcccgccc aaacactagt agtccaattc
      241 aatggggaat tattgagaaa agatggtgtt acaagtaaat actttacccc ttcatcaaca
      301 tatctagagt agattacacc ggaaataccc aacccgacca gaataaagta gatcaagaag
      361 ataattataa cttccctatt tatacttaaa aaaccattat taaaaataat ttttctttcc
      421 aaaatcagac ccacatttaa taagacaaca tgatttatta a
//
LOCUS       DAZFYC010000495          460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFYC010000495 DAZFYC010000000
VERSION     DAZFYC010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.502"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            138..>460
                     /gene="nleB"
                     /locus_tag="ACHKW5_005780"
     CDS             138..>460
                     /gene="nleB"
                     /locus_tag="ACHKW5_005780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO7514888.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSL"
BASE COUNT          166 a           70 c           87 g          137 t
ORIGIN      
        1 aaggatgtca tcgttaatga gactttttgt taatgtcaac aatgctatga atttatttta
       61 gaaaaacaat taagctgacg tagtgttccg tctaaatgac ggggcgatat taacatgatt
      121 agaacaagag gaattttatg ttatcttcat taaatgtcct tcaatccagc ttcagaggaa
      181 agacagcctt atcaaatagt acacttctcc agaaagtttc ttttgctgga aaagaatatc
      241 ctctggaacc tattgatgaa aaaaccccta ttctttttca gtggtttgaa gcaaggccag
      301 agcgatacga aaaaggagaa gtaccaatat tgaataccaa agaacatccg tatttgagca
      361 atattataaa tgctgcaaaa atagaaaatg agcgtataat cggtgtgctg gtagatggaa
      421 attttactta tgaacaaaaa aaggaatttc tcagtcttga
//
LOCUS       DAZFYC010000496          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFYC010000496 DAZFYC010000000
VERSION     DAZFYC010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.503"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          123 a           84 c          109 g          133 t
ORIGIN      
        1 gcataataaa aaaagccagc gactgacctg aaaaagaaga cgctggctaa aaggccttat
       61 atgtttgtag agacttattt ttcacagaca gcaatgatgc ctgtcaatat attatcaata
      121 tgcggattgt ttcagttaca gatgccttat taaggaaaaa aacagccagc actgactttc
      181 ggtggagagg tgctggctca gaaggataga tgtacttcac atgttgcttc tatatggcag
      241 tacattttct gacagacagt gacggatgtt gtcaagatat tgtgtcattt ataacctgaa
      301 tcaggggagg ccggaatgtt atctggcatt tttagcagag cctgaatgcc ataatcacgg
      361 ctcccggcgt tggccgtcag tgggtgacac tggcggcttt tttgtttttc tttactttca
      421 ttttctgtcg gcggtgacgg agacataca
//
LOCUS       DAZFYC010000497          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFYC010000497 DAZFYC010000000
VERSION     DAZFYC010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.504"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            240..431
                     /locus_tag="ACHKW5_005781"
     CDS             240..431
                     /locus_tag="ACHKW5_005781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514889.1"
                     /translation="MKNRKAKMLISRVYRRCYPSQWLRVSNRRVVLYLYSGIAREGIK
                     DKRSAAQNRWKNHLRTKGA"
BASE COUNT          140 a           90 c          117 g          101 t
ORIGIN      
        1 aaaaatggca gaactaacta aagaatggtt gcaacaaaca attactgata ttcagagcaa
       61 ttgaggtgag aaatgcggat ggaaaattgt ttattgccgt atctggtgcg gtttggggcg
      121 tcgtcattaa cagttattcc acatgtcatg atggctgaca atattatccc ggcaccaaag
      181 cgccataccg gtattgcagc ggcgcgacgt gcagcaaaga gacgcaggag agcaaaacga
      241 tgaaaaaccg taaagcaaag atgcttattt cccgtgtata cagacgttgc tatcccagcc
      301 agtggttgag agttagcaat cgccgtgtgg tgttgtactt atattctgga attgccagag
      361 agggaatcaa agataagcgc agcgcggcgc aaaaccgctg gaaaaaccac ttgcgcacta
      421 aaggagcttg atatggctat tgccgcaa
//
LOCUS       DAZFYC010000498          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFYC010000498 DAZFYC010000000
VERSION     DAZFYC010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.505"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(70..146)
                     /locus_tag="ACHKW5_005782"
     tRNA            complement(70..146)
                     /locus_tag="ACHKW5_005782"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Arg,seq:tcg)
     gene            complement(154..229)
                     /locus_tag="ACHKW5_005783"
     tRNA            complement(154..229)
                     /locus_tag="ACHKW5_005783"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Ile,seq:cat)
     gene            complement(270..383)
                     /locus_tag="ACHKW5_005784"
                     /pseudo
     CDS             complement(270..383)
                     /locus_tag="ACHKW5_005784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          112 a          131 c          111 g           94 t
ORIGIN      
        1 aaccactgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa caacacagtg
       61 tgcagataat ggaccgccat cgaggactcg aaccccgcgc aaccagcttc gaaggctgac
      121 gctctatccc gatgagctaa tggcggtatg tgatggtggc ccttgctgga tttgaaccag
      181 cgacctggcg attatgagtc gctcgctctc accactgagc taaagggccg ggcgcaggat
      241 aataacggta cgtaactaat tctgcaatat catccgttct gactgactac attctgaact
      301 tccctgaccg tctgctcaaa acgcccgctc tccagctcaa cgccaattgc acgacgcccc
      361 agcgccattg ctgctttgac gctacggaca taaaaaagcc agccactggg ggaggctggc
      421 aaactcgtag agcaaaatgc tgttacgc
//
LOCUS       DAZFYC010000499          436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFYC010000499 DAZFYC010000000
VERSION     DAZFYC010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.506"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>436
                     /locus_tag="ACHKW5_005785"
     CDS             <1..>436
                     /locus_tag="ACHKW5_005785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514890.1"
                     /translation="FKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRDAF
                     TGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPYAG
                     NMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNG"
BASE COUNT          154 a           81 c           92 g          109 t
ORIGIN      
        1 aatttaaaga tttacctcct tcaatccagg agattgcagc acacacactt cgtcatcgtc
       61 tgaacgaact tgaattggaa tcggtaacga aaaaagacac tgataatatg gctcgtaatg
      121 tgcgcgatgc gtttaccgga ttgtatttct gtgcgtctat aaataaacac gactcagaga
      181 gtgtggcaaa taaaattgca gaaacgacag cgcaaaacat caatacgaaa ccaacggaag
      241 aagaaattga tcagtttgct catgatgctg gtttaaaaaa caagaaagaa aaatcgccat
      301 atgcggggaa catgtttgtt tatgacaatc tcatcagaat tcgtggcgaa attccggcgg
      361 aatacctggc aagagtccat caggcattgc ttaaaaattt ggaaacagaa ttatttgatg
      421 gcaacactaa cggttt
//
LOCUS       DAZFYC010000500          432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFYC010000500 DAZFYC010000000
VERSION     DAZFYC010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.507"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>432
                     /locus_tag="ACHKW5_005786"
     CDS             <1..>432
                     /locus_tag="ACHKW5_005786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO7514891.1"
                     /translation="LSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPEN
                     YNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYD
                     QILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQ"
BASE COUNT          163 a           79 c           75 g          115 t
ORIGIN      
        1 ttatcggcta ttcatcacca gatcattgaa aaccaactgg atacagaaac aataactatt
       61 agttatgaat ttatcctctg tcaacatctt atcgcaaata ccgagttcag ctatttggca
      121 ctccctgaaa attataaccg tttgttttta ccaaattcaa aaaaccaaac aaataatcgt
      181 ttcaaaacgt taaacagcaa agctattggc aggctacttg ctgctggtgg cgtatacaat
      241 gggaacattg aaggattcag agatactgcg gaaaaactgg gtggagatgc aataaaaggc
      301 tatgatcaaa tactaaatga aaaaacagcg ggcatagcga tagcaacagc atctattctt
      361 ttaacaaagc gttctaatgt tgatacatat acagaaataa atagttactt aggcaaactt
      421 agaggtcaac aa
//
LOCUS       DAZFYC010000501          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFYC010000501 DAZFYC010000000
VERSION     DAZFYC010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.508"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..390
                     /locus_tag="ACHKW5_005787"
     CDS             <1..390
                     /locus_tag="ACHKW5_005787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO7514892.1"
                     /translation="GWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIK
                     VTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTGVKAGK
                     VSIPVISGNGQFATVAEVTVTEAGAAG"
BASE COUNT          122 a           96 c          126 g           87 t
ORIGIN      
        1 ggctgggtca gcagtatcgg taaggccgta acggcgaagg aagtgatcac ccgtacggtg
       61 aaagtgacca acgtgggtaa accttccgtg gcggaagaac gcagcgaaat tacgccggcc
      121 actgcaatta aggtgacacc gacatccggt accgtggaaa aaggaaaaac aaccaccctg
      181 actgtttctt ttgagccgga aagtgcaacc gacaagacgt tcagagcggt ttccgccgat
      241 ccgtcaacgg gaaccattgc tgtgaaagat atggtgatca ctgtgacggg ggttaaggct
      301 ggaaaagtga gtatccccgt gatttccggt aatggtcagt ttgccacggt agctgaagtc
      361 accgttactg aagcaggcgc tgcagggtaa acggaggtca tacatgtttc tgaaaacaga
      421 acaatttgaa t
//
LOCUS       DAZFYC010000502          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFYC010000502 DAZFYC010000000
VERSION     DAZFYC010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.509"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..229)
                     /locus_tag="ACHKW5_005788"
     CDS             complement(<1..229)
                     /locus_tag="ACHKW5_005788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7514893.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELT"
BASE COUNT          128 a          113 c           61 g          120 t
ORIGIN      
        1 ctgtcagttc agctctggcc cgaagcagcc tttcctccag atcgccctcg tcatcaggta
       61 ttctctgatt gtgttgtcgc cgctcgcgat ctatctccag tacagtttta acgtcataaa
      121 aaacttccct cccccgacgc tcaacaggag gaacgcccca tttatcaaat gcctgaacag
      181 atataccgat ggaggaggcc atatcacttt tattcaataa aaaggccatc tcctctccat
      241 aagccatcga taaaaagcga tacaacaacc atgtgttttt acaaaaccat ttgatatcat
      301 tgacattttt cgcattgacg acatcaaaac acatcgtaag gttgttgtat ttattttatt
      361 ttcaccttac ttatcaatta gatatgccaa acaattaaac aacaaccacc ccctcaaaaa
      421 at
//
LOCUS       DAZFYC010000503          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFYC010000503 DAZFYC010000000
VERSION     DAZFYC010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.510"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..269
                     /locus_tag="ACHKW5_005789"
                     /pseudo
     CDS             1..269
                     /locus_tag="ACHKW5_005789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          128 a           85 c          120 g           87 t
ORIGIN      
        1 gtatcagtga agaaacacaa aatgacagct gtgagaaaat actgacgccg acagagtgga
       61 aactactgtg ggttaagctg gaaggaaagc agcttccggc gcaaacaccg accctgaaat
      121 gggcctgttt gaaactggct aaactggggc gatggcatga cagcaaacgc acaagcagcc
      181 ccggttgggt agttatgtgg gatggctggt tcagacatca ggatatggct gagggttacc
      241 tggtgatgaa gtctcttgat caggagatct gctcaagaga caggtcaaag atgggggaca
      301 acgtctggtg ctgtataaca gcacaggcct cacactcttg acgcccgtgg aatatattag
      361 agaacaaaaa tttcaatatg tggtggacgc atacagagga ttgacagcat tcccatacta
//
LOCUS       DAZFYC010000504          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFYC010000504 DAZFYC010000000
VERSION     DAZFYC010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.511"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..191)
                     /locus_tag="ACHKW5_005790"
     CDS             complement(<1..191)
                     /locus_tag="ACHKW5_005790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7514894.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGG"
     gene            294..419
                     /locus_tag="ACHKW5_005791"
                     /pseudo
     CDS             294..419
                     /locus_tag="ACHKW5_005791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT           97 a          120 c           96 g          106 t
ORIGIN      
        1 cctccatcac cgcccccggt atcccgctca tgctggcgaa cccagacacg cagagtctcc
       61 ggcgtacagc caatctttgg agcaatggaa caaattgtcg cccattgtga gtcatattcg
      121 ctctgacttt ccagaaccat acggactgcc cgttgacgga cttcggggga aaaacgagta
      181 tttttagtca tcctgtttac ctctttctca ggaagtttag tctccaggat tcccggggcg
      241 gttcagtact caccaataaa taagcgtcaa tacggtgctc cgttgacgct tacgcagatc
      301 tgttacttca gttatctacg gcacaacgtc agggccgtta taaaacgacg cttcagcgtg
      361 gagtaatggc cccccgcctg ctcatcattg atgaaatagg ctatctgccg ttcagtcag
//
LOCUS       DAZFYC010000505          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFYC010000505 DAZFYC010000000
VERSION     DAZFYC010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.512"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..355)
                     /locus_tag="ACHKW5_005792"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHKW5_005792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT           97 a          110 c           86 g          121 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atct
//
LOCUS       DAZFYC010000506          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFYC010000506 DAZFYC010000000
VERSION     DAZFYC010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.513"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>409
                     /gene="nleB2"
                     /locus_tag="ACHKW5_005793"
     CDS             <1..>409
                     /gene="nleB2"
                     /locus_tag="ACHKW5_005793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO7514895.1"
                     /translation="GKEYELKVIDEKTPILFQWFEPNPERYKKDEVPIVNTKQHPYLD
                     NVTNAARIESDRMIGIFVDGDFSVNQKTAFSKLERDFENVMIIYREDVDFSMYDRKLS
                     DIYHDIICEQRLRTEDKRDEYLLNLLEKELREIS"
BASE COUNT          150 a           47 c           88 g          124 t
ORIGIN      
        1 ggaaaggaat atgagttaaa ggtcattgat gaaaaaacgc ctattctttt tcagtggttt
       61 gaacctaatc ctgaacgata taagaaagat gaggttccaa tagttaatac taagcagcat
      121 ccctatttag ataatgtcac aaatgcggca aggatagaga gtgatcgtat gataggtatt
      181 tttgttgatg gcgatttttc agtcaaccaa aagactgctt tttcaaaatt ggaacgagat
      241 tttgaaaatg taatgataat ctatcgggaa gatgttgatt tcagtatgta tgacagaaaa
      301 ctatcagata tttatcatga tattatatgt gaacaaaggt tacgaactga agacaaaaga
      361 gatgaatact tgttgaatct gttagagaaa gagttgaggg aaatttcaa
//
LOCUS       DAZFYC010000507          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFYC010000507 DAZFYC010000000
VERSION     DAZFYC010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.514"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..>405
                     /locus_tag="ACHKW5_005794"
     CDS             65..>405
                     /locus_tag="ACHKW5_005794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7514896.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDS"
BASE COUNT           99 a           90 c          126 g           90 t
ORIGIN      
        1 gaggagggaa ggaggttgtg acagttattg atggtatcag aggacatttc aggaggtgac
       61 cacgatggca ggtaattttg ccgatctgac agccgtgctg acactggatt ctgcccgttt
      121 ttctgaagag gcagcgcggg taaaaaaaga gctgggtgaa accagtgcgc ttgctgattt
      181 gatgtccggg aaagtcagtc agtctttcag aaaacaggct gatgctgctg agcagagtct
      241 gagccgacag gcgctggctg cacaaaaagc cgggatatca gtcggacagt ataaggctgc
      301 catgcgcaca ctgcccgcac agttcacgga tattgtcact cagcttgccg gtggtcagaa
      361 tcccttcctt atcatgctgc agcagggggg gcagatcagc gattc
//
LOCUS       DAZFYC010000508          400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFYC010000508 DAZFYC010000000
VERSION     DAZFYC010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.515"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>400
                     /locus_tag="ACHKW5_005795"
     CDS             <1..>400
                     /locus_tag="ACHKW5_005795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO7514897.1"
                     /translation="SSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIA
                     PSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQ
                     LEINRNKQQAAVTASKPKLDLTNTDWIYGVD"
BASE COUNT          111 a          107 c          109 g           73 t
ORIGIN      
        1 atcctctgac cagccagaaa acgatctttc tgtggttaaa ccggatgctg caattcagag
       61 cggcagcaag tgggggacag cagaagacct gaccgccgca gagtggatgt ttgacatggt
      121 gaagactatc gcaccatcag ccagaaaacc gaattttgct gggtgggcta acgatatccg
      181 cctgatgcgt gaacgtgacg gacgcaacca tcgcgacatg tgtgtactgt tccgctgggc
      241 atgccaggac aacttctggt ccggtaacgt gcttagcccg gccaaactcc gcgacaagtg
      301 gacccagctc gaaatcaacc gtaacaagca acaggcagcc gtgacagcca gcaaaccaaa
      361 actcgacttg acaaacacag actggattta cggggtggat
//
LOCUS       DAZFYC010000509          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFYC010000509 DAZFYC010000000
VERSION     DAZFYC010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.516"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>398
                     /locus_tag="ACHKW5_005796"
     CDS             <1..>398
                     /locus_tag="ACHKW5_005796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7514898.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKP"
BASE COUNT           88 a          102 c          120 g           88 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagcc
//
LOCUS       DAZFYC010000510          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFYC010000510 DAZFYC010000000
VERSION     DAZFYC010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.517"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>394
                     /gene="espL"
                     /locus_tag="ACHKW5_005797"
     CDS             <1..>394
                     /gene="espL"
                     /locus_tag="ACHKW5_005797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HFO7514899.1"
                     /translation="KIKLEKEVNKSIKWVKDIQDIELVLMQDIMNKVHSSLTNALHSL
                     DTSSRINWDDLLNEVVRETLSHNNIVGAIKITKNPDIKLDPGEANNIQLINDADAPLN
                     KIIIENEYMRITLDPLEQINILLNSFKEN"
BASE COUNT          162 a           51 c           65 g          116 t
ORIGIN      
        1 taaaataaaa ttagagaaag aagttaacaa atcgataaaa tgggttaaag atattcagga
       61 tatcgagttg gtcttaatgc aggacataat gaataaggtt cattcgtcac tgactaatgc
      121 attacattct ttagacacat catcaagaat taattgggat gatttactta atgaagtcgt
      181 aagggaaacg ctttcccata acaatattgt tggagctatt aagatcacta aaaacccaga
      241 cattaaactt gatcctggag aggctaataa tatacagctt attaatgatg cagacgcacc
      301 acttaataag ataataatag aaaatgaata tatgagaata actttagatc cgttagaaca
      361 aataaatatt ttattgaatt catttaaaga gaat
//
LOCUS       DAZFYC010000511          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFYC010000511 DAZFYC010000000
VERSION     DAZFYC010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.518"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..389
                     /locus_tag="ACHKW5_005798"
                     /pseudo
     CDS             1..389
                     /locus_tag="ACHKW5_005798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
BASE COUNT          134 a           89 c           92 g           74 t
ORIGIN      
        1 gcggtaatgg gtgacgcaac ctaccaagag acattcaacg aagaaagcca ggatgaagcc
       61 cggaaaaaaa tccggaggaa atggaaaatg ccgtgctccc gaataagcaa tgcaccgaag
      121 gcgatcaaca tgccaatggc aataatgaaa caggcgagac agcaaatccc ttaattacgg
      181 tgaacggtca tcacgttatc acatccacca gcaggacgtg tgaccatcta atgatcgacc
      241 ttaaaaccat gggaaaaaat cctgatgccc cgattatctc aataggtgca atatttttcg
      301 atccgcaaac cggagatatg ggaccggaat ttagtaagac tatcgatctg gaaactgctg
      361 gcggagtcat tgatcgggac accattaaa
//
LOCUS       DAZFYC010000512          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFYC010000512 DAZFYC010000000
VERSION     DAZFYC010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.519"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          115 a           87 c           58 g          118 t
ORIGIN      
        1 cttagcgtgg gaaaatttcc gttttccaag cggccccctc aagcggaccc cttatagtac
       61 aataattttc gatatccaaa ctgaccccaa ttacatctcc agtctctggc agcagagacc
      121 atatatttcc attgccgccg tagccacgat attctggcta ttcacaaaaa caatttagcc
      181 ccaaagcgca gtaaaaaagt acactatgaa ggtgtatgat accttcatag tgcttaagta
      241 attatgagta atataatctg tgtaaactac taattgcttc accgtcagtg attaaatgcg
      301 tagctgttcc ttgtcttata taatacctat tttcaaaatg acagacttct ccgacacttt
      361 ttatttcgat ttttaaaa
//
LOCUS       DAZFYC010000513          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFYC010000513 DAZFYC010000000
VERSION     DAZFYC010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.520"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..205
                     /locus_tag="ACHKW5_005799"
     CDS             <1..205
                     /locus_tag="ACHKW5_005799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514900.1"
                     /translation="NIIETNSNGWFPDTDGALITGLTFLDPKDATQVQGLFRHLQVRF
                     GDGPWQDVKGLDEVGSDTGRTGE"
     gene            207..>377
                     /locus_tag="ACHKW5_005800"
     CDS             207..>377
                     /locus_tag="ACHKW5_005800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514901.1"
                     /translation="MNILKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVV"
BASE COUNT          104 a           76 c          118 g           79 t
ORIGIN      
        1 aaacattatt gagacaaaca gcaatggctg gttcccggat acagatggcg cactcatcac
       61 cggactgacc tttcttgacc ccaaagatgc cacacaggtt caggggctgt ttcggcattt
      121 gcaggtcagg tttggtgacg ggccgtggca ggatgttaag gggctggatg aagtgggcag
      181 tgatacaggc agaacaggag aatgacatga atatactaaa aaaacttatg cagcgtctgt
      241 gtggttgcgg aaagcatgat gaccgtgaaa acggggagtt acttacagca caactgcggc
      301 tgggaccggc agacatcctg gagtccgatg agaatggtat tattccggag caggacaggg
      361 taatcacgca ggtggtg
//
LOCUS       DAZFYC010000514          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFYC010000514 DAZFYC010000000
VERSION     DAZFYC010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.521"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>377
                     /locus_tag="ACHKW5_005801"
     CDS             <1..>377
                     /locus_tag="ACHKW5_005801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO7514902.1"
                     /translation="TGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIK
                     PHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIK
                     MVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT           91 a           96 c          107 g           83 t
ORIGIN      
        1 actggcgttg aaatgttccg caaactgctg gacgaaggcc gtgctggtga gaacgtaggt
       61 gttctgctgc gtggtatcaa acgtgaagaa atcgaacgtg gtcaggtact ggctaagccg
      121 ggcaccatca agccgcacac caagttcgaa tctgaagtgt acattctgtc caaagatgaa
      181 ggcggtcgtc atactccgtt cttcaaaggc taccgtccgc agttctactt ccgtactact
      241 gacgtgactg gtaccatcga actgccggaa ggcgtagaga tggtaatgcc gggcgacaac
      301 atcaaaatgg ttgttaccct gatccacccg atcgcgatgg acgacggtct gcgtttcgca
      361 atccgtgaag gcggccg
//
LOCUS       DAZFYC010000515          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFYC010000515 DAZFYC010000000
VERSION     DAZFYC010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.522"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>372
                     /locus_tag="ACHKW5_005802"
     rRNA            <1..>372
                     /locus_tag="ACHKW5_005802"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           86 a           79 c          126 g           81 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gt
//
LOCUS       DAZFYC010000516          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFYC010000516 DAZFYC010000000
VERSION     DAZFYC010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.523"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..348
                     /locus_tag="ACHKW5_005803"
     CDS             <1..348
                     /locus_tag="ACHKW5_005803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO7514903.1"
                     /translation="LFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSR
                     KGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNIS
                     PAAFREKYHQMAA"
BASE COUNT          105 a           89 c           90 g           86 t
ORIGIN      
        1 ctgtttatgg cgctcaggag ccagcgccca cctgccgggc taatccacca ctctgatcga
       61 ggttcacagt actgcgcata cgattaccgg gtcatacagg agcagtttgg tctgaaaaca
      121 tcaatgtcgc gtaaaggtaa ctgttacgac aacgctccga tggaaagctt ctggggaacg
      181 ctgaaaaatg agagcctgag ccactatcgt tttaataacc gggatgaagc catctcagta
      241 atacgggaat acattgagat tttctacaat cgtcagcgtc gtcactctcg tctggggaat
      301 atctccccgg cagccttcag ggaaaaatat catcagatgg ctgcttaaaa aaagaacaaa
      361 tggtagtgtc
//
LOCUS       DAZFYC010000517          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFYC010000517 DAZFYC010000000
VERSION     DAZFYC010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.524"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            149..334
                     /locus_tag="ACHKW5_005804"
     CDS             149..334
                     /locus_tag="ACHKW5_005804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7514904.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
BASE COUNT          117 a           74 c           63 g          116 t
ORIGIN      
        1 gttgagtatc acaggcacat gtattttagc cccctgagtc tacagacaac caatattact
       61 taaataagtg ataatcttaa tgctgttttt tagaacagat gattgatatt tttgggccgg
      121 agaaacgcag acaggctttg ttgaataaat cgagcttttg ctgagtttaa ggatcagatc
      181 actcattttc ctgcaaaagc tcattgggcg aaacaatctg actcagagaa cccggattaa
      241 acgtctggcc cgcagaacca tctatttctc acgcactgta gtacgtaata aaaaagtcat
      301 cggtactttt atcaaaaaac atatattcta ttaattattg ccatcacctt tttcttattg
      361 acaacattac
//
LOCUS       DAZFYC010000518          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFYC010000518 DAZFYC010000000
VERSION     DAZFYC010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.525"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>369)
                     /locus_tag="ACHKW5_005805"
     CDS             complement(<1..>369)
                     /locus_tag="ACHKW5_005805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514905.1"
                     /translation="IDSIPVSNSVVCNPFYSLINKREVLQSIDKVHYSMNSSHHAEVF
                     VYAWLHKMLDGIKGQYKDVNEIEDVSELPSDKLITIHYMVKGEASVYRYSKGMRITDS
                     KGVLGLGSPGLYYMDFLSVFS"
BASE COUNT          127 a           75 c           52 g          115 t
ORIGIN      
        1 gaaaatacgc tcaaaaaatc catgtagtaa agaccaggag accccaaacc caaaactcct
       61 tttgaatctg ttattctcat accttttgag taacggtaaa cacttgcctc tcctttaacc
      121 atgtaatgaa ttgttataag cttatcggaa ggtagttcac ttacatcttc gatctcattt
      181 acatctttat attggccttt aattccatca agcattttat gaagccaagc atagacaaac
      241 acttcagcat gatgtgaact attcattgaa taatgcactt tatcaattga ctgcaagact
      301 tctctcttat taatcaatga atagaatgga ttgcagacaa ctgaatttga cacaggaata
      361 gaatcaata
//
LOCUS       DAZFYC010000519          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFYC010000519 DAZFYC010000000
VERSION     DAZFYC010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.526"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>368
                     /locus_tag="ACHKW5_005806"
     CDS             <1..>368
                     /locus_tag="ACHKW5_005806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514906.1"
                     /translation="LGEGTIIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALW
                     GSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNM
                     IAQGNPMPVPYGEMLVGSRR"
BASE COUNT           71 a          100 c          120 g           77 t
ORIGIN      
        1 ctgggtgagg gaacgatcat ccacattgtg ccgcgactgg ccggggccgg aaagggtgga
       61 ctgcagattg tgctgggggc ggcagccatc gtgggctctt tcttcaccgc cggcgcaacg
      121 atggcgttat ggggttcagc cctggcagcc ggtggttttt ctgccaccac gatgctgttt
      181 tcacttggag ccagcatgat actgggtggt gtggcccaga tgctggcccc gaaggcaaaa
      241 acaccggatt accgcgcaac ggataacggc agacagaaca cgtacttttc gtcactggac
      301 aacatgattg cccagggtaa cccgatgccg gtgccttacg gggaaatgct ggttggctcc
      361 cgccgtat
//
LOCUS       DAZFYC010000520          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFYC010000520 DAZFYC010000000
VERSION     DAZFYC010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.527"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..282
                     /locus_tag="ACHKW5_005807"
     CDS             4..282
                     /locus_tag="ACHKW5_005807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514907.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
BASE COUNT          120 a           65 c          103 g           80 t
ORIGIN      
        1 gaagtgaacg atagctaccg acagtttgaa aactggtggt caaaagacaa aagccagttc
       61 acgggagacg atgaattaaa agagtttgcc tgggtgatat ggcaggcatc gcgctctgct
      121 attgaactgg atatcgactg gcccgaatcg aatgacgacc tttggaaaga tggtgaagaa
      181 ggtgcttatg cgatgggtta tgaggatggg cgtgacaaaa cggtaattgc agtaatgaaa
      241 gccatcaggg ccgcaggaat caaagaaaag aatttcgatt aagcaaatat cacttcaata
      301 aatcgctttt aaggcatcac aatcgctctg tggtgaggta agcacgtgca aggcatgccg
      361 ataagcag
//
LOCUS       DAZFYC010000521          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFYC010000521 DAZFYC010000000
VERSION     DAZFYC010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.528"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>365
                     /locus_tag="ACHKW5_005808"
     CDS             <1..>365
                     /locus_tag="ACHKW5_005808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514908.1"
                     /translation="IESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAG
                     RINARLEDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVC
                     RWFGTSDLGWENYDEDVSR"
BASE COUNT           91 a           90 c          113 g           71 t
ORIGIN      
        1 aatcgaatcg accgtgacca gcctgcaccg tgaggctgaa gcacagttcc ggcctgagct
       61 ggagaaaatc gtcagcggga ttgaaacagg ttttcgtggc acggccctgt acgccacaga
      121 aaacattgcc ggtcgcatca atgcccgcct ggaggatgag ggctttaccg taaaaatcag
      181 ttttccggca gtcagccagt tacagacccg gctcgcggta aaaataaatc tgagtgcgct
      241 tatggaggaa agaacggaga ccgtcacccg tcgccgtcgg cagagtggcg tatggggaac
      301 cgtttgtcga tggtttggca ccagtgacct gggctgggaa aactatgacg aggatgtgag
      361 tcgca
//
LOCUS       DAZFYC010000522          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFYC010000522 DAZFYC010000000
VERSION     DAZFYC010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.529"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          108 a           67 c          103 g           87 t
ORIGIN      
        1 cagagcgcga gaaacagatg gttctgcgag tcagacgttt gattcaggtt ctcaggggca
       61 gattcaggga aagatcacag ggaaagatca tcggtgtgcc gtaggcacaa agatgcaaaa
      121 cgggacgttt tgcatgtaat aagcataagc ttgtgaagag atggtggcag gcccaccggg
      181 atctgctgtc ggaagtcggt cccaaacaac cccggctgct gtggtaaaga gccatggtag
      241 tgagcgcagg gggaacgatc agacgagatc tgatcctatg tgagattagg gaaagatcat
      301 caaaagttca ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc
      361 tcatt
//
LOCUS       DAZFYC010000523          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFYC010000523 DAZFYC010000000
VERSION     DAZFYC010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.530"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..197
                     /locus_tag="ACHKW5_005809"
     tRNA            122..197
                     /locus_tag="ACHKW5_005809"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT          102 a           82 c           83 g           93 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaacga aagttgttcg tgagtctctc aaattttcgc aactctgaag
//
LOCUS       DAZFYC010000524          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFYC010000524 DAZFYC010000000
VERSION     DAZFYC010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.531"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHKW5_005810"
     CDS             <1..>356
                     /locus_tag="ACHKW5_005810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7514909.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZFYC010000525          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFYC010000525 DAZFYC010000000
VERSION     DAZFYC010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.532"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>355
                     /locus_tag="ACHKW5_005811"
     CDS             <1..>355
                     /locus_tag="ACHKW5_005811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7514910.1"
                     /translation="PQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELI
                     ALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFF
                     DLLSDESIIKKLKYP"
BASE COUNT          113 a           43 c           72 g          127 t
ORIGIN      
        1 tacctcaatc aaatatctgg ccagtatcta tttattatcg tttgctttca tttgattatt
       61 tttctgcgcg attggattca ttattatatc ttgatgctga tatcgtctgt aagggttcat
      121 tgaacgagtt aatagcatta gagtttaaag atgaatatgg ggcagtggta attgatgtag
      181 atgctatgca aagtaaaagc gctgagcgtt tgtgtaatga ggattttaac ggtagctatt
      241 ttaactctgg tgtaatgtat attaatttac gggaatggtt aaaacaaaga ctaacggaaa
      301 aattctttga tctattatca gatgagtcaa ttataaaaaa attaaagtac ccgga
//
LOCUS       DAZFYC010000526          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFYC010000526 DAZFYC010000000
VERSION     DAZFYC010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.533"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>348)
                     /locus_tag="ACHKW5_005812"
     CDS             complement(<1..>348)
                     /locus_tag="ACHKW5_005812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514911.1"
                     /translation="TTKIKHARSVIDHIDFNCDQSKIAETLFFNLNKEPYKNSPELQE
                     LIWEKLVVYVNDFNLSNQEKSRLILRLFDDVKLLFNEVSVSILVNDIFLKDFFMKQPD
                     FAKWYFYQLIKKY"
BASE COUNT          119 a           62 c           49 g          118 t
ORIGIN      
        1 tcatacttct taattaattg atagaagtac cacttcgcaa aatcaggttg cttcatgaaa
       61 aaatccttca aaaaaatatc atttactaaa atgctgacag acacttcatt aaatagcaac
      121 ttaacatcat caaataacct tagtattaag cgtgattttt cttgattgct taagttaaaa
      181 tcattgacat atacaaccaa cttctcccaa atcaattcct gtaactcagg gctgttttta
      241 tagggttctt tattgaggtt gaaaaataat gtctcagcta ttttagactg atcgcagtta
      301 aaatcaatat gatcgatcac ggagcgggca tgctttatct ttgtagta
//
LOCUS       DAZFYC010000527          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFYC010000527 DAZFYC010000000
VERSION     DAZFYC010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.534"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKW5_005813"
     CDS             complement(<1..242)
                     /locus_tag="ACHKW5_005813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7514912.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          106 a           70 c           75 g           95 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcaga
//
LOCUS       DAZFYC010000528          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFYC010000528 DAZFYC010000000
VERSION     DAZFYC010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.535"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>341)
                     /locus_tag="ACHKW5_005814"
     CDS             complement(<1..>341)
                     /locus_tag="ACHKW5_005814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO7514913.1"
                     /translation="FENNNSHTGVVTEPILGKLIGQGSTAEIFEDMNDSSALYKKYDL
                     VGNQNNEVLEMARQESALFNTFYGDDASVVIQYGGDVYLRMLRVPGIPLSDIDTADIP
                     DNLESLYLQLI"
BASE COUNT          110 a           77 c           56 g           98 t
ORIGIN      
        1 catatcaact gcagataaag gctctctaga ttatcaggaa tatcagctgt atcaatatca
       61 ctaaggggaa tccctggcac gcgtagcatt cggagataca catcaccgcc atactgtata
      121 acaactgatg cgtcatcgcc ataaaaagta ttaaaaaggg cagattcttg cctagccatt
      181 tccagaactt cattgttctg gttaccaaca agatcatact ttttatataa agcagacgaa
      241 tcattcatat cttcaaagat ttctgctgtt gaaccttgcc ctattaactt accgagaata
      301 ggctccgtta ctacgccagt atgagaatta ttattctcaa a
//
LOCUS       DAZFYC010000529          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFYC010000529 DAZFYC010000000
VERSION     DAZFYC010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.536"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..>336
                     /locus_tag="ACHKW5_005815"
     CDS             17..>336
                     /locus_tag="ACHKW5_005815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514914.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFG"
BASE COUNT           95 a           62 c          105 g           74 t
ORIGIN      
        1 acgaggtaaa ttttcgatgg aaactgtatt gcatgcactg aaagcgatgg gaaaagccaa
       61 ttctgttgaa ctggcggcgc ggcttgatat cagccgtgaa gaagttctta acgaactgtg
      121 ggaactcaaa aaaaatggcg ttgttgataa aacgggtcac acctggtttc tggctgtcga
      181 aggtgaagcc ggggtaaccg aagggcaggc actacaacct gaagcgccgg atgtggtaac
      241 cgaagaggtc gctccaaaag ttaccgcaga catgatggtt gagtttatcg gtcaggatgg
      301 tgctaaaacg tgtgaggaac tggcgggtaa gttcgg
//
LOCUS       DAZFYC010000530          334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFYC010000530 DAZFYC010000000
VERSION     DAZFYC010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.537"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>334)
                     /locus_tag="ACHKW5_005816"
     CDS             complement(<1..>334)
                     /locus_tag="ACHKW5_005816"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7514915.1"
                     /translation="GGQAAGAFDCADMIYRLRQQKPVWALCNDTACSAAMLLASACSR
                     RLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQFEALPAEVRQDM
                     QQRIDAARR"
BASE COUNT           68 a          110 c           99 g           57 t
ORIGIN      
        1 cggcgcgccg catcaatgcg ctgctgcatg tcctggcgaa cctctgccgg taaggcttca
       61 aactgattgc catccacctt gtgcgcccct gagtaaatca gcgtgatatc cacaccggcc
      121 tgcgccagat gaccggcata gctgacatgg ctcatcatca cgccaatgga gccgatacgg
      181 gatgtctggg taaccagccg tcgggagcag gccgacgcca gcagcatggc tgcagaacag
      241 gccgtgtcat tgcacagtgc ccagaccggc ttctgctgac ggaggcggta aatcatgtca
      301 gcgcagtcaa acgcgccggc ggcctgcccg cccg
//
LOCUS       DAZFYC010000531          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFYC010000531 DAZFYC010000000
VERSION     DAZFYC010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.538"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..>333
                     /locus_tag="ACHKW5_005817"
     rRNA            1..>333
                     /locus_tag="ACHKW5_005817"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           96 a           72 c          103 g           62 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgcgcgat acagggtgac agc
//
LOCUS       DAZFYC010000532          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFYC010000532 DAZFYC010000000
VERSION     DAZFYC010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.539"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..182
                     /locus_tag="ACHKW5_005818"
     CDS             <1..182
                     /locus_tag="ACHKW5_005818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514916.1"
                     /translation="GWFPDTDGALITGLTFLDPKDATQVQGLFRHLQVRFGDGPWQDV
                     KGLDEVGSDTGRTGE"
     gene            184..>331
                     /locus_tag="ACHKW5_005819"
     CDS             184..>331
                     /locus_tag="ACHKW5_005819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7514917.1"
                     /translation="MNILRKLMERLCGCGKHDDSENGELLTAQLRLGPADILESDENG
                     IIPEQ"
BASE COUNT           84 a           66 c          109 g           72 t
ORIGIN      
        1 atggctggtt cccggataca gatggtgcgc tcatcaccgg actgaccttt cttgacccca
       61 aagatgccac acaggttcag gggctgtttc ggcatttgca ggtcaggttt ggcgacgggc
      121 cgtggcagga tgttaaggga ctggatgaag tgggcagtga tacaggcaga acaggagaat
      181 gacatgaata ttttgagaaa gcttatggag cgtctgtgtg gttgcggaaa gcatgatgac
      241 agtgaaaacg gggagttact tacagcacag ctgcgactgg gaccagcaga cattctggag
      301 tccgatgaga atggcattat cccggagcag g
//
LOCUS       DAZFYC010000533          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFYC010000533 DAZFYC010000000
VERSION     DAZFYC010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.540"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>328
                     /locus_tag="ACHKW5_005820"
     CDS             <1..>328
                     /locus_tag="ACHKW5_005820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514918.1"
                     /translation="WGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNG
                     AHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAA
                     DDGSERL"
BASE COUNT           65 a           92 c          119 g           52 t
ORIGIN      
        1 tgggggctga agttgccgac gttgcgccag cgcctgttcc gctgcgtgag tatccgtgag
       61 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tacccgaaaa agaagccatc
      121 gtggataacg gggcgcactt tgacggcgac cagagcggca cggtgaatgg cgtcacgccg
      181 cccgcggtgc agcacctgac tgccgaagtc accgcagaca gcggggaata tcaggtgctg
      241 gcgcgctggg acacgccgaa ggtggtgaag ggcgtgagct ttatgcttcg cctgaccgtg
      301 gcagcggatg acggcagtga gcggctgg
//
LOCUS       DAZFYC010000534          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFYC010000534 DAZFYC010000000
VERSION     DAZFYC010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.541"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>327
                     /locus_tag="ACHKW5_005821"
     CDS             <1..>327
                     /locus_tag="ACHKW5_005821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7514919.1"
                     /translation="QALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILL
                     QQGGQVKDSFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKT
                     LVLSGN"
BASE COUNT           59 a           96 c          104 g           68 t
ORIGIN      
        1 ccaggcgctg gctgcacaaa aagccgggat ttccgtcggg cagtataaag cggccatgcg
       61 aaccctgccc gcacagttta cggatatcgc cacgcagctt gccggtggtc agaatccctg
      121 gctgatcctg ctgcaacagg gcggtcaggt gaaggactcc ttcggcggga tgatccccat
      181 gttcaggggg cttgccggtg cgatcaccct gccgatggtc ggggtcacct cgctggcggt
      241 ggcgacaggt gcgctggcgt acgcctggta ccagggggat tccacgcttt cagcgtttaa
      301 taaaaccctg gttctttccg gtaatca
//
LOCUS       DAZFYC010000535          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFYC010000535 DAZFYC010000000
VERSION     DAZFYC010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.542"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..317)
                     /gene="nleC"
                     /locus_tag="ACHKW5_005822"
                     /pseudo
     CDS             complement(30..317)
                     /gene="nleC"
                     /locus_tag="ACHKW5_005822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
BASE COUNT           93 a           65 c           72 g           90 t
ORIGIN      
        1 ccatagctta cggcatcacg aaaagtttat gagcggctca aagcatcaag tatagtatta
       61 ctaatcatat cgaccgtatg ctgatcaatg agtctgccac gactgtcatg gacggcgcgt
      121 attacacttt cgtatacgtt gcttaaatct gctgcggaaa gtggtattcg cttacctata
      181 tccaaaacgt aatccgcata ggcattttca gcacgattag gagcaatggg agcagagtat
      241 cctgccgggg cggaaaagtt gaagttggac tgtaatgagg gaattttcat gtttatatct
      301 aataccctgc aaatcattta
//
LOCUS       DAZFYC010000536          317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFYC010000536 DAZFYC010000000
VERSION     DAZFYC010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.543"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>317
                     /locus_tag="ACHKW5_005823"
     CDS             <1..>317
                     /locus_tag="ACHKW5_005823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514920.1"
                     /translation="PQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIK
                     QLARRSTVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIA
                     KKLL"
BASE COUNT           70 a           86 c           93 g           68 t
ORIGIN      
        1 ccacaggtcg gcccttcatc tgagccggac tggttctacg tggttgtgct tgccggacag
       61 tccaacggca tggcctacgg tgaagggctt ccgttaccgg attcttacga tgctccggat
      121 ccgcgcatta aacagctggc gcgccgcagc acggtaactc cgggtggaga gagttgtacg
      181 tataacgaca tcattccggc tgaccactgc ctgcatgatg tgcaggatat gagtacgctg
      241 aatcatccga aggcagacct gagcaaaggg cagtacggct gtgtcggcca gggcttacat
      301 attgccaaaa aactgct
//
LOCUS       DAZFYC010000537          310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFYC010000537 DAZFYC010000000
VERSION     DAZFYC010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.544"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..236)
                     /locus_tag="ACHKW5_005824"
                     /pseudo
     CDS             complement(1..236)
                     /locus_tag="ACHKW5_005824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299329.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT           81 a           65 c           65 g           99 t
ORIGIN      
        1 aatcatacgg ttttttcccc gttgtgactc ttgaaacatt ttggccaaga atattaagag
       61 tatagtcatt aacggatttc attcctaaaa aaacaagtaa tccttctttc tcttgcgctt
      121 tttcgattgt tctccctgct aattattggg atggattgag attgttttgt cggaactgat
      181 acgctggcgc taatataaag acggcgcaat ggccgccaca ggttctttct gctcatccgg
      241 cgttatttct acaggtaata attcaacgcc agtgtcagaa gaggttgtta ccggaagacg
      301 ccgcgaaaca
//
LOCUS       DAZFYC010000538          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFYC010000538 DAZFYC010000000
VERSION     DAZFYC010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.545"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>303
                     /locus_tag="ACHKW5_005825"
     CDS             <1..>303
                     /locus_tag="ACHKW5_005825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7514921.1"
                     /translation="EHLALLKRRAEQAESSGNLQVSVEDLVRTGAFLVAMSLWHNHPQ
                     KTQSPSMNEAVMKIEQEVLTTWPADAIARAEDVVLCLSGMIEAVRPDTDITEVAKNN"
BASE COUNT           76 a           77 c           91 g           59 t
ORIGIN      
        1 gagcatcttg ccctcctgaa acggcgtgca gaacaggcag aatccagcgg caacctgcag
       61 gtaagcgtgg aagatctcgt cagaaccggc gcgtttctgg tggcgatgtc cctgtggcat
      121 aaccatccac agaaaacgca gtcaccgtca atgaatgagg ccgtgatgaa gatagagcag
      181 gaagtgctca ccacctggcc tgccgatgcc attgcccggg cggaagacgt ggtgttgtgc
      241 ctgtccggga tgatcgaagc tgttcgtccg gatactgata ttactgaagt ggcgaaaaat
      301 aac
//
LOCUS       DAZFYC010000539          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFYC010000539 DAZFYC010000000
VERSION     DAZFYC010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.546"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..>301
                     /locus_tag="ACHKW5_005826"
     CDS             44..>301
                     /locus_tag="ACHKW5_005826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7514922.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILKK"
BASE COUNT           94 a           68 c           70 g           69 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaaccca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa
      301 a
//
LOCUS       DAZFYC010000540          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFYC010000540 DAZFYC010000000
VERSION     DAZFYC010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.547"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>293)
                     /locus_tag="ACHKW5_005827"
     CDS             complement(<1..>293)
                     /locus_tag="ACHKW5_005827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7514923.1"
                     /translation="QIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRN
                     SLLFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGV"
BASE COUNT           80 a           75 c           69 g           69 t
ORIGIN      
        1 acgcccgatg aaaagcgttt cagcgtgaga gtattgtcac gatgtttacg acccagccat
       61 ggggaaaggt ctttcaggca tggcacgttc cgaatcgtcg cctccacgtg agacttcata
      121 aaatcttcag cggcagaatc cgtgggctga aaaagcagac tgtttcggga tttatgctca
      181 ataaaatacc cgaccacccc cagcaacatc tttgtatagc caacacgggc agatttaatc
      241 aggttaaccg tgcgaacctg gtcgttaccc atacagttca taatggcgat ctg
//
LOCUS       DAZFYC010000541          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFYC010000541 DAZFYC010000000
VERSION     DAZFYC010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.548"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>293
                     /locus_tag="ACHKW5_005828"
     CDS             <1..>293
                     /locus_tag="ACHKW5_005828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514924.1"
                     /translation="CTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEI
                     CDDDYAGISIGGCVLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGNPVSVEV"
BASE COUNT           60 a           76 c          101 g           56 t
ORIGIN      
        1 tgtaccagtc gggggcaggc acaccgagcc gggctgtggc tgattaaaac ggaactgctg
       61 gaaacgcaga ccgtggattt cagcgtgggc gcagaagggc ttcgccatgt gccgggcgat
      121 gttattgaaa tctgcgatga tgactatgcc ggtatcagca tcggtggttg cgtgctggcg
      181 gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc atcctccggc
      241 accacgctga taagcctggt tgacgggcag ggtaatccgg tcagcgtgga ggt
//
LOCUS       DAZFYC010000542          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFYC010000542 DAZFYC010000000
VERSION     DAZFYC010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.549"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..292
                     /locus_tag="ACHKW5_005829"
     CDS             <1..292
                     /locus_tag="ACHKW5_005829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7514925.1"
                     /translation="HTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHS
                     LKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT           80 a           60 c           83 g           69 t
ORIGIN      
        1 tcacacggac cgtggaggcc agtactgttc agcagattat caggcgcaac tgaagcggca
       61 taatctgcgt ggaagtatga gcgcaaaagg ttgctgctac gataatgcct gcgtggaaag
      121 cttctttcat tcgctgaaag tggaatgtat ccatggagaa cactttatca gccgggaaat
      181 aatgcgggca acggtgttta attatatcga atgtgattac aatcggtggc ggcggcacag
      241 ttggtgtggc ggcctcagtc cggaacaatt tgaaaacaag aacctcgctt ag
//
LOCUS       DAZFYC010000543          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFYC010000543 DAZFYC010000000
VERSION     DAZFYC010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.550"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..144)
                     /locus_tag="ACHKW5_005830"
     CDS             complement(<1..144)
                     /locus_tag="ACHKW5_005830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO7514926.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEA"
     gene            complement(122..>289)
                     /locus_tag="ACHKW5_005831"
     CDS             complement(122..>289)
                     /locus_tag="ACHKW5_005831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7514927.1"
                     /translation="LETVRRWVRECRIFPPPVKDGREYLFHESAVKVDLNRPVTGSLL
                     KRIRNGKKAKS"
BASE COUNT           63 a           81 c           55 g           90 t
ORIGIN      
        1 tgcttcagtg attgctatcc tcctgtctcg gcctaaacca aactctttac ccgtccttgg
       61 gtccctgtag cagtaatatc cattgtttct tatataaagg ttagggggta aatcccggcg
      121 ctcatgactt cgccttcttc ccatttctga tcctcttcaa aaggctacct gttactggtc
      181 gatttaagtc aacctttacc gctgattcgt ggaacagata ctctcttcca tccttaaccg
      241 gaggagggaa tatcctgcat tcgcgcaccc atcgacgaac tgtttcaag
//
LOCUS       DAZFYC010000544          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFYC010000544 DAZFYC010000000
VERSION     DAZFYC010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.551"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>285
                     /locus_tag="ACHKW5_005832"
     CDS             <1..>285
                     /locus_tag="ACHKW5_005832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7514928.1"
                     /translation="CTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEI
                     CDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPIS"
BASE COUNT           61 a           81 c           93 g           50 t
ORIGIN      
        1 tgtaccagcc gggggcaggc acaccgcgcc gggctgtggc tgattaaaac agaactgctg
       61 gaaacgcaga ccgtggactt cagcgtgggt gccgaagggc ttcgccatgt gccgggcgat
      121 gtcattgaaa tctgtgatga tgactatgcc ggtatcagca ccggcgggcg cgtgctggcg
      181 gtaaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc atcctccggt
      241 accacgctga taagcctggt tgacggaagt ggcaatccga tcagc
//
LOCUS       DAZFYC010000545          277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFYC010000545 DAZFYC010000000
VERSION     DAZFYC010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.552"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..232)
                     /locus_tag="ACHKW5_005833"
     CDS             complement(<1..232)
                     /locus_tag="ACHKW5_005833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514929.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELT"
BASE COUNT           69 a           88 c           49 g           71 t
ORIGIN      
        1 ctgtcagttc ccatctggca ataagcaatt tctcctcata gtcgtcttcg ctatcgccat
       61 caggcacatc gtttttactt ctcctcagat acgatatgta aaaatcgcgc caggcatcca
      121 gatccagttg ccctcgctta ttcgatatcg gggcacccgg caatttctgc aatctgcgaa
      181 gctggcgatc ggtcagactt aaatgcctgg caacttcagt ctgcgtagcc actcctcacc
      241 tcgcaaaaac tctcacctca caatcacaac aaaaccg
//
LOCUS       DAZFYC010000546          276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFYC010000546 DAZFYC010000000
VERSION     DAZFYC010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.553"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            199..>276
                     /locus_tag="ACHKW5_005834"
     CDS             199..>276
                     /locus_tag="ACHKW5_005834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514930.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPM"
BASE COUNT           74 a           61 c           66 g           75 t
ORIGIN      
        1 agtgaataaa gtcaacaaga ttgtttcatg aagaggcacc gtaatggtgc ctttgtcatt
       61 tctgcgcttc gcacaagcgt aaataaacca aagaaccttt caggatgagc cctggtggat
      121 aaccggcagt ggtctggtta accctctttg ggctggttat tcctgtgcgc agggttcatc
      181 actaaaagga aataaccgat gaatatgatg accgtgccgt ttcacggcga ttctctttat
      241 gtggttaacc ataacggcga accatacgtt cccatg
//
LOCUS       DAZFYC010000547          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFYC010000547 DAZFYC010000000
VERSION     DAZFYC010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.denovo.554"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..95
                     /locus_tag="ACHKW5_005835"
     tRNA            19..95
                     /locus_tag="ACHKW5_005835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHKW5_005836"
     tRNA            138..213
                     /locus_tag="ACHKW5_005836"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           71 a           62 c           73 g           67 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aag
//
LOCUS       DAZFYC010000548         9951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.guided.160, whole genome shotgun sequence.
ACCESSION   DAZFYC010000548 DAZFYC010000000
VERSION     DAZFYC010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.guided.160"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..795
                     /locus_tag="ACHKW5_005837"
                     /pseudo
     CDS             40..795
                     /locus_tag="ACHKW5_005837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
     gene            complement(1067..1153)
                     /locus_tag="ACHKW5_005838"
                     /pseudo
     CDS             complement(1067..1153)
                     /locus_tag="ACHKW5_005838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            1483..2298
                     /gene="sul2"
                     /locus_tag="ACHKW5_005839"
     CDS             1483..2298
                     /gene="sul2"
                     /locus_tag="ACHKW5_005839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO7514931.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2359..3162
                     /locus_tag="ACHKW5_005840"
                     /note="aph(3'')-Ib"
     CDS             2359..3162
                     /locus_tag="ACHKW5_005840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO7514932.1"
                     /translation="MNRTNIFFGKSHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3162..3998
                     /locus_tag="ACHKW5_005841"
     CDS             3162..3998
                     /locus_tag="ACHKW5_005841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO7514933.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4144..5535)
                     /locus_tag="ACHKW5_005842"
     CDS             complement(4144..5535)
                     /locus_tag="ACHKW5_005842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HFO7514934.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLTVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(5572..6144)
                     /locus_tag="ACHKW5_005843"
     CDS             complement(5572..6144)
                     /locus_tag="ACHKW5_005843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7514935.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            complement(6281..6826)
                     /locus_tag="ACHKW5_005844"
     CDS             complement(6281..6826)
                     /locus_tag="ACHKW5_005844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFO7514936.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGI
                     SYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFC
                     ISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQL
                     SRLDLASPVLEPEVIWLGRRR"
     gene            complement(6962..7144)
                     /locus_tag="ACHKW5_005845"
                     /pseudo
     CDS             complement(6962..7144)
                     /locus_tag="ACHKW5_005845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            7306..7920
                     /locus_tag="ACHKW5_005846"
     CDS             7306..7920
                     /locus_tag="ACHKW5_005846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7514937.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKRE
                     ERPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATID
                     TTTAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMA
                     SMGQSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
     gene            complement(8229..8885)
                     /locus_tag="ACHKW5_005847"
     CDS             complement(8229..8885)
                     /locus_tag="ACHKW5_005847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HFO7514938.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            9280..9951
                     /locus_tag="ACHKW5_005848"
                     /pseudo
     CDS             9280..9951
                     /locus_tag="ACHKW5_005848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2396 a         2538 c         2568 g         2449 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg ttaaaagcaa caaaaatgac
       61 ttcgttgatg ctgaagctat ctgtgaagca gcatcacgtc catctatgcg tttcgtgcag
      121 cccagaaccg aatctcagca ggcaatgcga gctctgcatc gtgtccgtga atccctggtt
      181 caggataagg tgaaaacaac taatcagatg catgcttttc tgctggaatt tggtatcagc
      241 gttccgcgag gtgctgccgt tattagtcga ctgagtaccc ttcttgagga cagtagtttg
      301 cctctttatc tcagccagtt actgctgaaa ttacaacagc attatcacta tcttgttgag
      361 cagattaaag atctggaatc tcagttgaaa cgaaagttgg acgaagatga ggttggacag
      421 cgcttgctga gtattccctg cgttggaacg ctgactgcca gtactatttc aactgagatt
      481 ggcgacggga agcagtacgc cagcagccgt gactttgcgg cggcaacagg gctggtaccc
      541 cgacagtaca gcacgggagg tcggacgaca ttgttaggga ttagcaagcg gggcaacaaa
      601 aagatccgaa ctttgttggt tcagtgtgcc agggtattca tacaaaaact ggaacaccag
      661 tctggcaagt tggccgactg ggtcagggag ttgttgtgtc ggaaaagcaa ctttgtcgtc
      721 acctgtgctc tggcaaacaa gctggccaga atagcctggg cactgacggc gcgacagcaa
      781 acttacgaag cataaaggca gaaatacacc agtttaaaca atcattcatc tggttttgcg
      841 aatactgata ttgatgatac taacggccca ccggcctgtt gaggaacctg taaaacggaa
      901 aggctcattg aagccgtata ttttctggag gttcatcagg cgcggaactc atcgaggcgc
      961 gggaataaaa tcccattcag acgccggata gattcaagca agccaacttg tcgtcaaaat
     1021 cggtgttgca aaaacgggag tgaccataga ttccgttttc tgaggcgacc cccacataga
     1081 gaccttcccg gtcattcact ttgtagagtt tgtccctggg cttgagattg cgcagcttgg
     1141 tatcggtcag catgtacctt gtccttcttc gtctccgata cccactctat tgtaaacaag
     1201 acatttttat cttttatatt caatggctta ttttcctgct aattggtaat accatgaaaa
     1261 ataccatgct cagaaaaggc ttaacaatat tttgaaaaat tgcctactga gcgctgccgc
     1321 acagctccat aggccgcttt cctggctttg cttccagatg tatgctattc tgctcctgca
     1381 gctaatggag caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc
     1441 gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc
     1501 attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg
     1561 ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac
     1621 ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg
     1681 cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc gctcgacagt
     1741 tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct caatgatatt
     1801 cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc caaactcgtc
     1861 gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc tggcgacatc
     1921 atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg tgccggtatc
     1981 aaacgcaacc gccttgtcct tgatcccggc atggggtttt ttctgggggc tgctcccgaa
     2041 acctcgctct cggtgctggc gcggttcgat gaattgcggc tgcgcttcga tttgccggtg
     2101 cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg tccgggggat
     2161 gtcggggccg cgacactcgc tgcagagctt gccgccgccg caggtggagc tgacttcatc
     2221 cgcacacacg agccgcgccc cttgcgcgac gggctggcgg tattggcggc gctgaaagaa
     2281 accgcaagaa ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga
     2341 aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtaaatc gcattctgac
     2401 tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg tgacgggcat
     2461 gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga gcgtgaccgc
     2521 ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg gcaggaggaa
     2581 caggagggtg catgcttggt gataacggca attccgggag taccggcggc tgatctgtct
     2641 ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc tgttcacagc
     2701 ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg acgcgccgtt
     2761 gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga caagagtacg
     2821 ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct cgaccaagag
     2881 cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat ggtggaccct
     2941 aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc agatcgctat
     3001 gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc agatgaagca
     3061 gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga ccgcgaacgc
     3121 cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg ccgcctgttt
     3181 ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt tccagcctcg
     3241 tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg aaacctataa
     3301 aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat gggaggggag
     3361 cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc ggggagcgaa
     3421 tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt gcagcggaac
     3481 taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt ctcccgatcc
     3541 gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca ggttgtcaaa
     3601 ctgactacgt ccacgcggcg attatagccg atcaaatgat gagcaatgcc tcggaactgc
     3661 gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc ggctggctgg
     3721 tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat atgttctacg
     3781 atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag atggcggacg
     3841 cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg tacgcttatg
     3901 ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat ctagctatcg
     3961 cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac ttctcctatc
     4021 ccctgggaac acatcaatct caccggagaa tatcgctggc caaagcctta gcgtaggatt
     4081 ccgccccttc ccgcaaacga ccccaaacag gagagagcat aaaaaatatt ggggcagcgt
     4141 gagctatgct gccatcgctg gtcccttccc gtcactttga aaacccggat accagcagac
     4201 aaatttatcc tgcagttcgt tattcaaaac ggcggtccgg gtctgtaaca aataatgagc
     4261 cccttttctg ctccactgca tctgctgctt tttggccatt cgtctggcga tcacttcatt
     4321 gatcgtggat tccacaaacg cggttgatac cggctctccg taccgacgca tttcgccgta
     4381 gtttgggatc atcatcttat tattccggat gtaggtatac atttcatcca gatgtttttg
     4441 cagggatttc aggctaggat agctgagttc agggtcatcg caatacataa cgcaattatc
     4501 aatatgttcc agcgcagcga caacattgcc atgccagaga tatcgtttaa tgctttccag
     4561 cagtgccaga actttactcc ccgcctctgg atctgatacc agcagtcccc gggcatattg
     4621 catgagcacc gtcagcctca tggtgatatg aaaccagtcc agcacatgcg ttgactcagg
     4681 gtacataccg aactggaggt ccctgagatt atccgcgccg tcggacagaa aaaatatctg
     4741 ctggttggcc tgcattccct gcgctgaaag gtgggtcatg agcctgcgtt ccggatgaca
     4801 gtcatccttc tgaacaaaac cgaagcggcg ggtgtcagca ggcgcaccaa cggaaaaaga
     4861 cttcccggca ataatttcaa aattacgctt tttgtcatcc cgatcgcgaa cataaccacc
     4921 atctataccc accacaagcg gttttcctgg cctgggcagg ttcccccagt cgcgggggca
     4981 gccggaaaga aaacctgaat gagcttcagc ctcagcgtca agacgctgcg ccacctggca
     5041 taaatgattc ctcaccgttg aggcattcag gctgtggccc accggcagaa tatctttcag
     5101 cagacgagtc gtcatttcat aggagatcat cgaggcccag cgggtttcaa tatatttcag
     5161 tgccgggtga gaataatcgc cggcccagtc gctgagcaaa ctgactgtct ttgtatcact
     5221 ctcttcacaa cggcaccggt acacccgaag cccggatacc ggaataacgc caaacagcgt
     5281 ccggtaccgt attttctgtt tgcctttgat tctgcgcgca gcaaggcagt gagggcaccg
     5341 gatatggtgt tgagtatatt cgtctgcctg cagctggacc accgactgct ggaccgtatt
     5401 cagcaacagc ttggattctg acaccgataa tccgatgtcg ttccgggtct cccctgattt
     5461 ctggattgtc atcagttcct ccgttcgact ggagcctgat tcatcggtga tgactatctg
     5521 gagcgtcagt tgcatcattg cctcctgctg tggtggatga ctatctcccg gctactggtt
     5581 gttcatctgg tgacgaagct tataatatgt ggaaacgcca aggcccgcat ggtcgcaggc
     5641 gatcctgagc ctgacccctc tgcttctgag tgcgtcaatt tcctggatct ttttgatatc
     5701 ggcgccgggg cgccctgttt tttcaccccg tgcttttttc gcggcgatgc cgtcggcctg
     5761 cctttccttc agtaaggctc gttcaaactg gctgaatgcc gacatcatgt gcagctgcag
     5821 ttcctgcatc ggattgtttg tgccggcgct gaacgtcagc tgctctttca gaaaaataag
     5881 ggtaacgccc tggtcacgaa gccgggtggt gacagcgcac atatccgaca tattccggca
     5941 aaggcgatcg atggaatgaa ccagaagcgt atcaccaggg cgtaattgcc ccaggagttc
     6001 ctgtaacccc gggcggtttg tgtccttcgc gctggcatgc tcaacacaaa ttttatcagg
     6061 ctgaaaaccc gcctttgaca atgcctcttc ctgacgggcc gtattctgat cggccgagct
     6121 taccctgatg taggcatatt gcacgcaaaa cctccggaag gtctttaaac atcatagaga
     6181 tttaaagaat aatccataaa cactgattta gggaggttta tagacatttt ttcggggatc
     6241 tgagaggcat gctgaaaacg tataccttta tagacatcag tcagcgacga cggcccagcc
     6301 agataacttc aggctccagt accggagatg caaggtcgag cctgctcagt tgccggttga
     6361 ttttgtggcg gttgaacctg acagcatcca ggtcgtcagc aaaagggcgg atctcgccga
     6421 ccgttgtttc atcatccgca ttaacgatag cttcaagaaa cgccagggtt ttatcgaact
     6481 catccgcagc tgtggctccg ggcataccat ggccgcttat acaaaacggc gctttcagcg
     6541 tagagttttc ataaatggcc tcaacacgaa tgtcatgaag ccagtgctca aagaaattgt
     6601 attcataggt aaagcgatcg ccagcatcaa aagcaaaatc atcaatcacg acccggaacg
     6661 gattatcgac aaaaccgatg cctccttcgt aggagatacc gtaatctttg ccataaatgt
     6721 gaaattgatg gagatggtcg tccccccagc cctgcactat ctggaaaatg aagtgaagcg
     6781 cggccagtga cgtgtcggca gcaattctca gccgacgcca gaccatcgga ctgactccat
     6841 gaactgctat tttgatgacg tagatcttca tagtcagcag gtattgttat gaaatgatgg
     6901 tggttatttt aacccctgta gatggcatca ttcgatggct gccccgaggt ttttcatgct
     6961 ctccgatctg gatgatgcaa taaatttgag cgtgaagccc ccggcgactg caggccagcg
     7021 ccagttcata ttcattcagc gccagaaact ccagccgctg tgcatcatcg aaatcaggta
     7081 gaccatagag cgcttcctgc tcggcgtccg aaagaatact gaggagctta tttttagtag
     7141 gcatctctga tccttatgcc tatttaaacg cgcttgtcac attatcatta acatagaacc
     7201 tttacgtgtc ataatttaaa ttacgggtaa tgtgactacc gttgacagat tatggtaccc
     7261 aaatcagaat gtcacataat cgaacgtata tgtgacagag gaaatatgtt catcggatat
     7321 attcggatgt caaaagcgga tggttcgcag acgaatgatt tacaacgtga tgcactgatc
     7381 gccgcaggtg ttgatgccgc acatttttat gaggaccggg catcgggcaa acgagaagag
     7441 cgtcctggac ttacagcctg tctgaaagca ctacgacccg gagacacgct ggtcgtatgg
     7501 aagctggacc gcctcggtcg tgatcttcgt catctcatta atacagtgca cgacctgacc
     7561 gcgaggggga ccggtctgaa agtgctgacc ggtcacgggg cgactatcga cacaacaacg
     7621 gccgctggca agcttgtctt cggtatcttt gccgcactgg cggagtttga gcgcgaactg
     7681 attgctgaaa gaacgactgc aggtctggca tcagcaagag cccgtgggcg gaacggcggc
     7741 cgaccttata aaatgacgcc agtcaaactg cgactggcaa tggcatcaat gggacaatca
     7801 gaaacaaagg tcagtacgct atgccaggaa cttgggataa cccggcaaac tttgtacagg
     7861 catatttccc ctgttggtca actgcgggcg gacgggatca agctgttcaa ccgtggataa
     7921 tttgaacgga cggaaaaatc tggggttccg gcgtagtacc cctatcacct ccacattttt
     7981 gcaacagtgc cttataaatg gcagccttat gtttacttgc caagatgggc taccatatga
     8041 tgttttatac tgagcaatat taatattatc tttaatggaa aaattaagcc cactaagtga
     8101 cccactgcta tatggctcta ttgtgaagac gcctgagggt aaaaatttat acatggttgt
     8161 ccctatgtct attattgcaa ggtattcgtt ttataaattt gagcgtttaa aatcacacat
     8221 caaagcaatt agtcaggata aacaacaata cccagtgctt cgagaatcag ttcttgctgc
     8281 caggctgcaa ttttgatacc tgatgtatcg actttgcggg gatctaaacc gtcgagttcg
     8341 gcgtggcata aattggcacc ctgtaggcta aattgccccc agacatcttc ggaaaaaaca
     8401 cctcgactta agtctgactc tttcagtgat gcacccgcta ggttcgttcc tatccagcga
     8461 ttttcaaaca actcacattt ttctaaacaa accctctcca tattggcata ggaaagatta
     8521 catccagaaa taaatgctga gcaaaagtac atacgattac tcacttgatg ggcaaagttt
     8581 gttcgggaaa agttggcacc ttttaaatca cacgcacgga actctatacc gtagcaattg
     8641 gcattactga agtttgccat cgcaagttgg cattgttgga aacttgcatc acgaagatct
     8701 gcgacatcaa agtggcagcc ttcgatatca ccctgttcaa tgaacttgca gttgacgaat
     8761 gtcgtatcac gcaagttagc acgtcgaaag tcgctgcgaa tgaatgtgca agcggtgaag
     8821 gtgagatcac ttaagtcttt atgtgaaaag ttgtggtgcc gatatgtatg attgtaggtt
     8881 tccatgagta ctccttgttg aaaggagggc tagactacca ttatacatat gccaagacat
     8941 taaaataaaa gcctgttaca tcaacacatt acaacccctc aatttacctg ttttaagtgg
     9001 tcaaattggc gcgtagagcg cctatcgctc ttgcattggc acgcggccct gttgaaaaaa
     9061 gttattcgct ttagataggg ttaggagagc cgtgcttcgc cggtaaaaaa tcggactatc
     9121 cgacgcaggg cggactcatc ggcggagaag caaactttga ctatatctcc ctacacttgg
     9181 aagagggcta gactggcccc atagatcacc agacacttcg actctcttaa aataagaggt
     9241 agtcttggaa ctatgtttaa taccagtcag aacgataaga tggttggagt gttatcagga
     9301 cctgagcggc gtcggcgtcg tacaccgcag gaaaaaattg ccattattca gcagactatg
     9361 gagcccggta tgaccgtgtc tcatgtcgcg cgcctgcatg gcatcaatgc aaaccagata
     9421 ttcaaatggc gcaagcaata ttaagacggt tcactgacgg ctgttgcatc gggcgaggaa
     9481 gtggtgccgg cctccgaact cgctgccgcc aataagcaaa ttcgcgaact ccagcgcctt
     9541 ctgggcaaaa agacgatgga agccgagata ctcaaagagg ccgtggagtt cggccgggca
     9601 aaaaaatgga ttgcgcatgc gccctcgttg cctgggaacg acgaataagc gacgtttgca
     9661 gaagtcttgg cgtgtcgcgt gcgcagttga gcattcgggt tcaccggcca tctgactgac
     9721 aggatcgcag acggcagccc cgttctgacg ataccaccgt attgtcccgg ataaacatgg
     9781 cggtggctga cctgccgacg tatggttatc gccgtgtgtg ggcgctgctg cgacgggagt
     9841 cggagcggga cggcctgcct gtggttaatg caaagcgggt gtatcgcatt atgagaacgc
     9901 ataatctgct tcggcactgt tgcaaagtta gcgatgaggc agccttttgt c
//
LOCUS       DAZFYC010000549         7602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.guided.185, whole genome shotgun sequence.
ACCESSION   DAZFYC010000549 DAZFYC010000000
VERSION     DAZFYC010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.guided.185"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..1014
                     /locus_tag="ACHKW5_005849"
     CDS             109..1014
                     /locus_tag="ACHKW5_005849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFO7514939.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1011..2249
                     /gene="mrx(A)"
                     /locus_tag="ACHKW5_005850"
                     /gene_synonym="mrx"
     CDS             1011..2249
                     /gene="mrx(A)"
                     /locus_tag="ACHKW5_005850"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFO7514940.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2249..2833
                     /gene="mphR(A)"
                     /locus_tag="ACHKW5_005851"
     CDS             2249..2833
                     /gene="mphR(A)"
                     /locus_tag="ACHKW5_005851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFO7514941.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3326..4090)
                     /locus_tag="ACHKW5_005852"
     CDS             complement(3326..4090)
                     /locus_tag="ACHKW5_005852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFO7514942.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            4392..4949
                     /locus_tag="ACHKW5_005853"
     CDS             4392..4949
                     /locus_tag="ACHKW5_005853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7514943.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            5132..5992
                     /locus_tag="ACHKW5_005854"
     CDS             5132..5992
                     /locus_tag="ACHKW5_005854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO7514944.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(6103..6234)
                     /locus_tag="ACHKW5_005855"
     CDS             complement(6103..6234)
                     /locus_tag="ACHKW5_005855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7514945.1"
                     /translation="MSHNLDHSRMVKEVLKAVARGDGNAEVVVTYQGSDAQWNENSR"
     gene            6421..7602
                     /locus_tag="ACHKW5_005856"
     CDS             6421..7602
                     /locus_tag="ACHKW5_005856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFO7514946.1"
                     /translation="MCMNPFPQQNKSYQFYYDESNNVRKLYLSKQIDGYNIDHDPDKH
                     NSVNFVLAGVAHTGSSSSADFDDLRQRIQLQANAKEFKLKHLAKGDFLTMLTSKKLTA
                     FFEWLLYGDLYLHYFHLNMEYWGYVDIIDDCILFGREKGFIPEMSDEQFYGYMLANKD
                     ALHTYVKANKVPFIQFLKSYDFPYIEGREQAFIQGLLSQISAHCVNLYTATNRDEFQL
                     RLAHGLLQLLMACSDQNIDDMTLTMDIRDEPPTDDDNRLVDGFAIFYQNRAQMFEASS
                     HIFDIEKVVEADLQKAAEANPNLTLNYSFADSKLNPLVQVSDVVAGFMQHYFDYLNSH
                     SYEQIKHDRNSLNERQRLNMEFLRLLINKSHDNNPTLLHCVMSALEHEKHKTFTYPDE
                     P"
BASE COUNT         1657 a         2076 c         2079 g         1790 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga atgagcgaac
     1021 gtcgatatag cccgctcgcg acgctgttcg cggcgacctt tctcttccgg atcggcaacg
     1081 cggtggcggc cctcgcgctt ccatggttcg tcctgtctca tacaaagagc gcggcctggg
     1141 cgggcgccac ggccgctagc agcgtcatcg cgaccatcat cggcgcgtgg gttggtggtg
     1201 gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat ctcgggtgtg gtgggcggcg
     1261 tggccatggc gagcatccca ctgctcgatg ccgttggcgc cctctcgaac actgggctga
     1321 tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg tatggccgcg caggacagtg
     1381 agctgcccaa actcggccac gtcgccgggc tctccgttga gcgcgtctcg tcactgaaag
     1441 cggtgatcgg gaacgtcgcg attctaggtg gcccggccct tgggggggcc gcaatcggcc
     1501 tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg ctccgtcctt gcaggtctgc
     1561 tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat gaccacgacg gcgactctct
     1621 ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct gctgcgccct ctctttggta
     1681 tagtgatgat cttcgtgggc atcgttggcg ccaacggcag cgtcatcatg cctgcgctgt
     1741 ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt ctcctcaatg atgggggctg
     1801 gtggtctcct tggcattgcc attcatgcgt cggtcggcgc ccggatatca gcgcagaact
     1861 ggctggcggt ggcattttgt ggctctgcgg tgggctcgct tctgctttca cagttgccag
     1921 gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact gctgaccggc tcagtctctc
     1981 ccattctcaa cgctgccatc tacaaccgca cgccgccaga acttctcggc cgggtactcg
     2041 gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt tatgcttgcg gccggcgcgt
     2101 ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc ggccgtgttt gcggggctcg
     2161 tggctctact ctcgctccgt cttcaatttg ctacaatggc ggcggcagcc acagcctccg
     2221 ccccaaccca tacagaaggt gaacactgat gccccgcccc aagctcaagt ccgatgacga
     2281 ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt cccatagagt tcacgctcag
     2341 cggagtagca aaggaggtgg ggctctcccg cgcagcgtta atccagcgct tcaccaaccg
     2401 cgatacgctg ctggtgagga tgatggagcg cggcgtcgag caggtgcggc attacctgaa
     2461 tgcgataccg ataggcgcag ggccgcaagg gctctgggaa tttttgcagg tgctcgttcg
     2521 gagcatgaac actcgcaacg acttctcggt gaactatctc atctcctggt acgagctcca
     2581 ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc gcggtggtgg aggggatccg
     2641 caagcgactg cccccaggtg ctcctgcggc agctgagttg ctcctgcact cggtcatcgc
     2701 tggcgcgacg atgcagtggg ccgtcgatcc ggatggtgag ctagctgatc atgtgctggc
     2761 tcagatcgct gccatcctgt gtttaatgtt tcccgaacac gacgatttcc aactcctcca
     2821 ggcacatgcg taaacggagg tgtgcagagt ccctgcggca ggcgacgaac acgaccgtcg
     2881 tcgattagta ccggtacggt cggtggtatc gaagtcttga tcaccactca ggtctacggc
     2941 ttacaaatgg tgaccatccc gatacttgcg tcagagcacc gggccgattc tttgacagtg
     3001 aatcactccc gtaaggttgt gccggtgtgg gtgtcccggg tcgagacgat actccgccaa
     3061 tgcgcccagc aaacaacctg gccatcgcag gtggtgggga gcggtgtggc ggatgagttg
     3121 gacaagttgg tgtagcagca cgagcacggc gagataacat cgcaggagtt cgacatgctc
     3181 aagagacagc tgattgcgaa tcgcgatgca gattcataac ccgattgcgg gttggcttca
     3241 ctccaccatc accgagcaga ctagcacggc gggctctgtt gcaaagattg gcggcagtca
     3301 gaggtaggct gtcgctctgc gccgatcagg cggctgctgc gaaatggtgg ttgagcatgc
     3361 ccatggcctc cgtcagcgcc gagggcccaa tgccaaaagc tctctccaca aggcgcacct
     3421 cgcccctgat gccgggctgc aggcaccagg ggcgagcctg tcctttgcgc agggctcgca
     3481 tgacttcgaa tcccttgatc gtggcatagg ccgtggggat cgatttgaaa ccgcgcaccg
     3541 gcttgatcag tatcttgagc tttccgtgat cggcctcgat cacgttattg agatacttca
     3601 cctgccggtg ggccgtctcc cggtccagct ttccttcgcg cttcaattcg gtgatcgctg
     3661 caccatagct cggcgctttg tcggtattga gcgtggcagg cttttcccag tgcttcaggc
     3721 ctcgcagggc cttgcccagg aaccgcttcg ctgccttggc gctgcgggtc ggcgacaggt
     3781 agaaatcgat cgtgtcgccc cgcttgtcga ctgcccggta caggtaggtc cacttgcccc
     3841 gcaccttgac gtaggtttca tccaggcgcc agctcggatc aaagccacgc cgccagaacc
     3901 agcgcagccg cttctccatc tccggggcgt agcactggac ccagcgatag atcgtcgtat
     3961 ggtcgaccga aatgccgcgt tccgccagca tttcctcaag gtcgcgatag ctgatcggat
     4021 agcgacaata ccagcgcacc gcccacagga tcacatcacc ctggaaatgg cgccacttga
     4081 aatccgtcat cgttccgtcc gtccaatctc cgccaagcat gctcaagctt cacgattttt
     4141 gcaacagagc cagctgaagc tcatccggtt caccggtgaa tctgccatag ctttcagtct
     4201 gctcagtggt cagaaagtca acgggcatat cggcctccct gcctgacggg catttagtaa
     4261 catttttcca accgtacgaa atgttataaa ttatcggaca tcgtaaaact gttacattaa
     4321 tatgtctatt aaatcgtaaa tttgtaataa tagacatgag ttgtccgata ttcgatttaa
     4381 ggtacatttt tatgcgactt tttggttacg ctcgggtctc aaccagtcag cagtctcttg
     4441 atcttcaggt cagagcactc aaagacgcag gtgtgaaagc aaaccgtata tttaccgata
     4501 aggcatccgg cagttcaaca gaccgggaag ggctggattt gctgaggatg aaggtggagg
     4561 aaggtgatgt cattctggtt aagaagctcg accgtcttgg ccgcgacact gccgatatga
     4621 tccaactgat aaaggaattt gacgctcagg gcgtggcagt ccggttcatt gatgacggga
     4681 tcagtaccga cggtgatatg gggcaaatgg tggtcaccat cctgtcggct gtggcacagg
     4741 ctgaacgccg gaggatccta gaacgcacga atgagggccg acaggaagca aagctgaaag
     4801 gaatcaaatt tggccgcagg cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga
     4861 agggcactgg tgcaacggaa attgctcatc agctcagtat tgcccgctcc acggtttata
     4921 aaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4981 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctggta
     5101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacattttc gtgtcgccct
     5161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5341 taaagttctg ctatgtggtg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     5401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5521 cactgctgcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5641 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     5701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5881 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6121 ccactgagcg tcagacccct gatatgtgac aacgacttcg gcattgccat cccctctggc
     6181 gacggctttc agaacctctt tcaccattct gctgtggtcc aggttgtgtg acatgggaac
     6241 tcattctgga tggttactct gaaagctcat attctgccac accccgattt gcagccgcca
     6301 ggctgccgtg gttctaaatc aaatcctctc tcttaaagaa gcccgccctg tgcgggtttt
     6361 ttgtttatag acttccagat cctacattgt tatatatggt tcataaccca caaaaaagtg
     6421 atgtgtatga atcctttccc gcagcaaaac aaaagctatc aattctatta cgatgaatct
     6481 aataacgttc gtaaactata tctatccaaa caaattgatg gttataacat tgaccatgat
     6541 ccagacaagc acaacagcgt aaacttcgtg ttagccggtg ttgctcatac tggttcctca
     6601 tccagtgccg actttgatga tttacgccag cgcatacagc tacaggccaa cgccaaagag
     6661 tttaagctga aacacttagc caaaggcgac ttcctgacta tgctcacgtc taagaagctc
     6721 acagcgttct ttgagtggct tctctatggt gatctatacc tacactactt ccacctgaat
     6781 atggagtatt ggggatacgt ggacatcatt gatgattgta tcctgttcgg acgtgagaag
     6841 ggcttcatcc cggaaatgag tgatgaacag ttctatggct acatgttggc aaacaaggat
     6901 gctctacaca cctacgttaa ggctaacaag gttcccttca tccagttcct caagtcctat
     6961 gacttcccat acatcgaggg gagggaacag gctttcatac aagggctatt aagtcagatc
     7021 tccgctcatt gcgtaaacct ctatacagct accaatagag atgagtttca gcttcggtta
     7081 gctcatggcc tcctgcagct cctgatggcc tgctctgacc aaaacataga tgatatgaca
     7141 ctcactatgg atattaggga tgaaccgcct acagacgacg acaaccgcct tgttgatggt
     7201 tttgctattt tctaccagaa ccgggcgcaa atgttcgagg cttccagcca tatctttgac
     7261 atagagaagg tagtggaagc agatcttcaa aaggcagcag aagccaaccc taatctaacg
     7321 cttaattact ctttcgctga ttcaaagcta aatcctctgg ttcaagtgag tgatgttgtg
     7381 gctggcttta tgcagcacta ttttgattat ctcaatagcc atagctacga acagattaaa
     7441 catgacagaa acagccttaa cgaaaggcaa agattaaata tggagttttt aaggctcctg
     7501 ataaacaaat ctcatgataa taacccaaca ttattacatt gtgttatgtc agctttagag
     7561 catgaaaaac ataaaacatt tacttacccg gatgaaccat aa
//
LOCUS       DAZFYC010000550         5943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.guided.201, whole genome shotgun sequence.
ACCESSION   DAZFYC010000550 DAZFYC010000000
VERSION     DAZFYC010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.guided.201"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..334
                     /locus_tag="ACHKW5_005857"
     CDS             <1..334
                     /locus_tag="ACHKW5_005857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HFO7514947.1"
                     /translation="PGSRGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKY
                     TATQLSRTELGSRVICRDKQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTL
                     KNSGGKKG"
     gene            331..945
                     /gene="prfH"
                     /locus_tag="ACHKW5_005858"
     CDS             331..945
                     /gene="prfH"
                     /locus_tag="ACHKW5_005858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO7514948.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1002..2459)
                     /gene="pepD"
                     /locus_tag="ACHKW5_005859"
     CDS             complement(1002..2459)
                     /gene="pepD"
                     /locus_tag="ACHKW5_005859"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7514949.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2720..3178
                     /gene="gpt"
                     /locus_tag="ACHKW5_005860"
     CDS             2720..3178
                     /gene="gpt"
                     /locus_tag="ACHKW5_005860"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7514950.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3270..4514
                     /gene="frsA"
                     /locus_tag="ACHKW5_005861"
     CDS             3270..4514
                     /gene="frsA"
                     /locus_tag="ACHKW5_005861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7514951.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4572..4973
                     /gene="crl"
                     /locus_tag="ACHKW5_005862"
     CDS             4572..4973
                     /gene="crl"
                     /locus_tag="ACHKW5_005862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7514952.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5012..>5943)
                     /gene="phoE"
                     /locus_tag="ACHKW5_005863"
     CDS             complement(5012..>5943)
                     /gene="phoE"
                     /locus_tag="ACHKW5_005863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO7514953.1"
                     /translation="YMSDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKA
                     ESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFM
                     TKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDF
                     AISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGG
                     FANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNM
                     SAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1440 a         1468 c         1544 g         1491 t
ORIGIN      
        1 tcccggttca cgtggtgatt actcctggat tgtacagcct gttgcgaacg aaaaaacgct
       61 ccactcgctg gcgcatgggg cagggcgtaa atgggggcgc accgagtgta aagggcgtct
      121 ggcagcgaaa tacacggcga cgcaactctc gcgtactgaa cttggcagtc gggtaatttg
      181 tcgcgataaa caactcatct ttgaagaagc gccacaagcc tataaatcgg ctgaaagcgt
      241 ggtgcaatgt ctggtgcagg ccggtttaat tattcctgtc gcgcggctgc gtccggtgct
      301 aacgctcaaa aacagtggag ggaaaaaagg atgatcttgc tacaactctc ctcagcgcag
      361 gggccggaag aatgttgtct cgcagtgaaa aaggcgctgg acaggcttat taaagaagct
      421 gcccgacaag acgtcgcggt aacggtgttg gaaacagaaa cgggccgcta ctctgacacg
      481 ctgcgttcgg cgctgatttc tctggatggc gataacgcat gggcattaag cgaaagctgg
      541 tgtggcgcta ttcagtggat ttgtccgagt ccgtatcggc ctcatcatgg gcgcaaaaac
      601 tggtttctgg gcattgggcg ttttaccgct gatgagcagg aacaatcgga tgcaatccgt
      661 tatgagacgt tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg
      721 gtacgcgcca cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgtagtcag
      781 catgctaaca agcggctggc acgattgctg attgcctgga agctggagca gcagcaacag
      841 gaaaatagcg cggtgctgaa atcgcagcgg cgaatgttcc atcaccagat tgaacgtggc
      901 aacccgagac ggacatttac agggatggct tttatcgaag gataatgaag gatgaaactg
      961 ccggaaggcg attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct
     1021 ttcagcagtt cagtcagcag cgtccagtaa tgacctacgc tttcgatgtg aacttgctca
     1081 tccggagagt gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac
     1141 ggttttttga acagaccaca ttccaggccc gcgtggataa tctggatgtt cggtgttttg
     1201 ttgaacagac gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc
     1261 tgccagccag gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa
     1321 tccagcatgc tcacgacgta gtctttaccg ctgtcgatca gtgaacggat cagacagtga
     1381 atttctacgt tattatcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca
     1441 ccttttgcca cgtctgagtt gcggatcaca ccgttcgggg tggcgttcag cagacgaata
     1501 aaggtatcgc gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac
     1561 aaggccagat ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc
     1621 atcagagatt tcaggacgtc gactttatca gctgcgacag caatggtcgc aaaggcttca
     1681 cgcgggatgg cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag atccagttct
     1741 tctgcatgac ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg
     1801 atttccccgc cggagtgacc ccctttcaga ccttttaagg ttaacttgaa ggtttcaaaa
     1861 ccagctggaa ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca
     1921 caacccatgt agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc
     1981 aaccagttgc tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc
     2041 agcacttcca gcgggccgtg aaccacgttt tcgtcagcca gaactgccag tgcagaggcc
     2101 ataccaatgc cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca
     2161 atataaggct ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc
     2221 accatatcga ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca
     2281 ggtttacgaa tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt
     2341 gcccaaccaa caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa
     2401 cagattttgg caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg
     2461 ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa
     2521 tgttgtcaac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
     2581 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
     2641 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
     2701 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
     2761 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
     2821 cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     2881 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     2941 gaaacgtgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     3001 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     3061 aaaaccggcc ggtcgcccgc tggttgatga ttatgttgtt gatatcccgc aagatacctg
     3121 gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     3181 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     3241 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     3301 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     3361 cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg tatcgcatga
     3421 ttaaccgtct gatgtggatc tggcgcggta ttgacccacg tgaaatcctc gacgtccagg
     3481 cacgtattgt gatgagcgat gctgaacgta ccgacgatga tttatacgat actgtgattg
     3541 gctaccgtgg tggcaactgg atttatgagt gggccaccca ggcgatggtg tggcaacaaa
     3601 aagcctgtgc ggaagaagat ccgcaactca gtggtcgaca ctggctgcat gcggctacgt
     3661 tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag caagcgcagg
     3721 ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct gccgggcacg atgcggcaga
     3781 tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg
     3841 atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt
     3901 acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact attgatatgc
     3961 cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagcctg ttgcatcagc
     4021 acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg
     4081 gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga
     4141 aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc
     4201 aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg tttggggatg catgatgctt
     4261 ccgatgacgc gttgcgcgtg gagctgaatc gctattcatt aaaagtgcaa ggattgctgg
     4321 gacgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg
     4381 aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta gagatcccat
     4441 ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccggc tggatcgaaa
     4501 aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg taaaacagtt gcatcacaac
     4561 aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc aaaaaattta
     4621 ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt
     4681 gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc tggggctggt
     4741 ggatggagct ggaagcgcag gaatcccgtt ttacctacag ctaccagttt ggtctgttcg
     4801 ataaagcagg cgactggaag agcgttccgg taaaagacac tgaagtggtt gaacgactgg
     4861 agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg ctgaatctga
     4921 agctggaacc ggcggatgat tttcgtgacg agccggtgaa gttaacggcg tgagtgaaat
     4981 gtgccggatg catcacatcc ggcaataatc attaaaactg ataggtcatg ccaaccgcga
     5041 caatatcatc attattaata ttcaatttgt tatcgctatc cagttggttg attttataat
     5101 caacaaacgc tgacatattt ttgttgaaat aatatgtagc cccgacatcg atataattga
     5161 ccagatcttc atcaccgata ccttcaatat ctttcccttt cgataagaca taacccagcg
     5221 atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat
     5281 tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc agaataaaaa gttgccagat
     5341 aaatattatt ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc ttgccagtgc
     5401 cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggca ccactaatgg
     5461 cgaaatcgct gccgccaaag tcatatgtca atgacgtgcc gaagccatcg ccgttttgct
     5521 ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc aggccatcga
     5581 taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt ttggtcataa
     5641 agttgtcggt ctgcgccgag gagtcgccac caaattccgg gaacatatcg gtccaggctt
     5701 ccacgtcata caacgcgccc aggttacggc catagtcgaa agaacccaaa tctttatact
     5761 tcaatccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc gctttatttc
     5821 cggcaaactc cgcttcccaa cggccatagc cagtcagttg atcgttaatt tgtgtttcgc
     5881 ctttaaaacc aaaacggata taactctggt cgccatcttt actgtcgtta tcactcatat
     5941 aat
//
LOCUS       DAZFYC010000551         5763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.guided.204, whole genome shotgun sequence.
ACCESSION   DAZFYC010000551 DAZFYC010000000
VERSION     DAZFYC010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.guided.204"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..1211
                     /locus_tag="ACHKW5_005864"
     CDS             78..1211
                     /locus_tag="ACHKW5_005864"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7514954.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1300..1833
                     /gene="blc"
                     /locus_tag="ACHKW5_005865"
     CDS             1300..1833
                     /gene="blc"
                     /locus_tag="ACHKW5_005865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7514955.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1830..2147)
                     /gene="sugE"
                     /locus_tag="ACHKW5_005866"
     CDS             complement(1830..2147)
                     /gene="sugE"
                     /locus_tag="ACHKW5_005866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7514956.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2329..2475)
                     /gene="ecnB"
                     /locus_tag="ACHKW5_005867"
     CDS             complement(2329..2475)
                     /gene="ecnB"
                     /locus_tag="ACHKW5_005867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7514957.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2586..2711)
                     /gene="ecnA"
                     /locus_tag="ACHKW5_005868"
     CDS             complement(2586..2711)
                     /gene="ecnA"
                     /locus_tag="ACHKW5_005868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7514958.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2763..3329)
                     /gene="efp"
                     /locus_tag="ACHKW5_005869"
     CDS             complement(2763..3329)
                     /gene="efp"
                     /locus_tag="ACHKW5_005869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7514959.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3371..4399
                     /gene="epmB"
                     /locus_tag="ACHKW5_005870"
     CDS             3371..4399
                     /gene="epmB"
                     /locus_tag="ACHKW5_005870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7514960.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4789..5658
                     /locus_tag="ACHKW5_005871"
     CDS             4789..5658
                     /locus_tag="ACHKW5_005871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7514961.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         1537 a         1425 c         1401 g         1400 t
ORIGIN      
        1 tatcgttaca atctaacgta tcgccaatgt aaatccggcc gcctatggcg ggccgttttg
       61 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
      121 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
      181 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
      241 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
      301 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
      361 ctcgagggga aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta
      421 aacagtggaa tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat
      481 tgcaggtgcc ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
      541 agcctgcatg ggcgccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
      601 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa acacgtgtct
      661 tccagccact caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt
      721 acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg ttagatgctg
      781 aaacttatgg tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga
      841 atccccgtga tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct
      901 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa
      961 atcctgacag catcattaac ggcagtggca ataaaattgc actggcagca caccccgtaa
     1021 aagcgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa
     1081 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc
     1141 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg
     1201 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     1261 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     1321 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     1381 gtaacagtag tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc
     1441 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
     1501 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag
     1561 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
     1621 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
     1681 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     1741 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     1801 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     1861 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     1921 acaatgccgg tgatagccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     1981 cctactggta acgatttcat cgcccaggca agtagcgcca tactgacaat catcgccgtc
     2041 acggtaataa cactcggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     2101 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     2161 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     2221 tgaacggata ttttgccagg tggcagggga atttcaacta ttaccttagt gactatcgca
     2281 gaaaaaacaa acggcacgac atacctgtcg taccgttatt gcttattgct attgctgcgc
     2341 tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa atgtcttcac caacgccacg
     2401 cgtggtgttg caggcagtta atactgttga aagcaccaga acagaaaaga tcgctgcaat
     2461 tgtcttcttc accataacgt cttcctttta ggtaatgtta tttatgtttg cctatagcaa
     2521 attaataata gacaacatcg ccaaaaatga cggaaatctg atgatttttc ggaagagaag
     2581 aaaaattagc tggcagcgcg agagatggag ttgccgagat gtttgatgtc ttcgccgaaa
     2641 ccgcgagcgg tgttacagcc cgtgagcagc gtgctggcaa gcaaaacaag aacgataagg
     2701 cgtttcatca tctctgacct gaataagtga tggtgcagcc tgcaggccgc accacaaccg
     2761 cattacttca cgcgagagac gtattcacca gagcgggtat ccactttgat gacttcgccg
     2821 atttgtacga acagcggaac cttaaccaca gcgccagtag acagggtagc cggtttgccg
     2881 ccagtacctg cggtatcacc tttcaggccc ggatcggtat caacgatttc cagttcaacg
     2941 aagttcggcg gagtaacgga gatcggctga ccattccaca gagttacgat acactctgcc
     3001 tgatccagca gccatttagc gttgtcgcca attgcttttg catcagcaga cagctgctcg
     3061 aaagtttcgt tgttcatgaa gtgccagaac tcaccgtcgt tgtacaggta agtcaggttc
     3121 atatcgacaa catcagcgcc ttcagcggaa tcagtagatt tgaaggtttt ttctacgcga
     3181 gtaccggtca gcagacgacg cagtttaacg cgagcaaatg cctggccttt acccggtttt
     3241 acgaattcac tcgcttcaac cgcgtaaggt tcgccgtcta acatgatttt aagaccagca
     3301 cgaaaatcgt tgctatagta cgttgccata aggccctctg aaatttgtta attggtagct
     3361 aagccacaaa atggcgcata ttgtaaccct aaatacccca tccagagaag attggttaac
     3421 gcaacttgcc gatgttgtga ccgatcctga tgaacttctg cgtcttttga atatagacgc
     3481 ggacgaaaaa ctgttggccg gacgcagcgc caaaaagctg tttgccctgc gtgtgccccg
     3541 ctcatttatc gatcgcatgg agaaaggcaa tccggacgat cctcttttgc gtcaggtact
     3601 tacctcgcaa gatgagtttg tcgtcgcgtc cggattctcc accgacccgc tggaagaaca
     3661 gcacagcgta gtgcctggtt tgttgcataa ataccacaac cgggcgcttt tgctggtcaa
     3721 aggcggctgc gcggtaaatt gccgctattg cttccgtcgc cacttcccct atgccgaaaa
     3781 tcagggcaac aagcgtaact ggcagactgc tcttgagtat gttgctgcgc atccggaact
     3841 ggacgagatg attttctccg gcggcgatcc gctgatggcg aaagatcacg agctggactg
     3901 gttgctcaca caactggaag ccatcccgca tatcaaacgt ctgcggattc acagccgtct
     3961 gccgattgtg atcccggcac gtatcaccga ggcgctggtt gaacgctttg cccgttctac
     4021 gctgcaaatc ttgctggtga atcacatcaa tcatgccaat gaggtagatg aaacgttccg
     4081 tcaggcgatg gctaaattgc gccgtgtcgg tgtcaccctg cttaaccaga gcgttctgtt
     4141 acgtggtgtg aacgataacg cacaaacgct ggcaaacctg agtaatgcgt tgttcgatgc
     4201 cggcgtaatg ccgtattacc tgcatgtgct cgataaagta cagggcgcgg cgcattttat
     4261 ggtgagtgat gacgaagcac ggcagattat gcgtgagttg ctgacactgg tgtcgggtta
     4321 tctggtgccg aaactggcgc gagaaatcgg cggcgaaccc agcaaaacgc cgctggatct
     4381 ccagctacgc cagcagtaac tgattaaaca gggaagttaa acattagcct gatgcgctac
     4441 gcttatcagg cctacattgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg
     4501 ataaggcgtt tacgccgcat ccggcaagag caacaaacgc aagtacagca acctgtcatt
     4561 aaactgggaa atttaatctt tgcacgttgc ataaccttta attattcata tgcattgctg
     4621 ttgttacttt ttatatagct aacaccaata attaaccttt cttttctgtt tatatagagt
     4681 gagcacggct atttatgtta ttttaacaac tattactacc aagcccattt atctggctta
     4741 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
     4801 atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc actggcgctg
     4861 aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgtcat ggaactgcgc
     4921 gacctgctga ttgcgctgga aagtcgtatg catcaaaagc ataaactgga tgctgctgca
     4981 cgccttcagt atgagcaagc tcgcgataaa gtcattaaaa aaatggcaga aaacatccca
     5041 gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa cacgctggaa
     5101 cttaccgata atcaaggcga aaatcttacc ttcgtattaa cgctacatga cggtagcaaa
     5161 tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat cattcatgca
     5221 attaataatg cggaaatgcg tgaactggca ttacgtatta cctcactgtt agatttcctg
     5281 ccgttatatg acgttgactg ccaggaaaat ggcaatctgg aatatgacac ctattcccaa
     5341 ccggaatgga aacataacct gttcgaccat tatctggcgg tgctttaccg ctttaaagat
     5401 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt
     5461 aaagaaatag aggcgatcac tcgccgaatg cttgatttca gcccacgact gaaaaaactc
     5521 gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg cgaataacgc ccaaccgcta
     5581 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
     5641 gcaccacagg cgaaataatc tcatctttgc taatgcgagg ctggagccat ccagcctgaa
     5701 atgactgata acgtgatcgt tggtcatgcc ggatgcggcg taaacgcctt atccggccta
     5761 caa
//
LOCUS       DAZFYC010000552         5341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.guided.212, whole genome shotgun sequence.
ACCESSION   DAZFYC010000552 DAZFYC010000000
VERSION     DAZFYC010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.guided.212"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..307
                     /locus_tag="ACHKW5_005872"
     CDS             65..307
                     /locus_tag="ACHKW5_005872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO7514962.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(339..989)
                     /gene="tetR(A)"
                     /locus_tag="ACHKW5_005873"
     CDS             complement(339..989)
                     /gene="tetR(A)"
                     /locus_tag="ACHKW5_005873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO7514963.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1095..2294
                     /gene="tet(A)"
                     /locus_tag="ACHKW5_005874"
     CDS             1095..2294
                     /gene="tet(A)"
                     /locus_tag="ACHKW5_005874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO7514964.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2326..3210)
                     /locus_tag="ACHKW5_005875"
     CDS             complement(2326..3210)
                     /locus_tag="ACHKW5_005875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO7514965.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(3348..3740)
                     /locus_tag="ACHKW5_005876"
     CDS             complement(3348..3740)
                     /locus_tag="ACHKW5_005876"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFO7514966.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            3744..5341
                     /locus_tag="ACHKW5_005877"
                     /pseudo
     CDS             3744..5341
                     /locus_tag="ACHKW5_005877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
BASE COUNT         1012 a         1730 c         1650 g          949 t
ORIGIN      
        1 accttagcgt acgatttttt ccgaattctg cgggctcccc tatctcatct gcgcaaggca
       61 gaacgtgaag acggccgccc tggacctcgc ccgcgagcgc caggcgcacg aggccggcgc
      121 gcggacccgc gccacggccc acgagcggac gccgcagcag gagcgccaga aggccgccag
      181 agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa agcccgtagc
      241 gggctgctac gggcgtctga cgcggtggaa agggggaggg gatgttgtct acatggctct
      301 gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt
      361 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
      421 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
      481 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
      541 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
      601 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
      661 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
      721 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
      781 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
      841 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
      901 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
      961 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     1021 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     1081 tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc actgtcgcgc
     1141 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     1201 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttgatgc
     1261 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct
     1321 tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg gcgcctttcc
     1381 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     1441 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     1501 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     1561 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     1621 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     1681 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     1741 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     1801 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     1861 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     1921 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     1981 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     2041 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     2101 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     2161 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     2221 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     2281 gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag gcgacttccg
     2341 gcaagctata cgcgccctag gagtgcggtt ggaacgttgg cccagccaga tactcccgat
     2401 cacgagcagg acgccgatga tttgaagcgc actcagcgtc tgatccaaga acaaccatcc
     2461 tagcaacacg gcggtccccg ggctgagaaa gcccagtaag gaaacaactg taggttcgag
     2521 tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca
     2581 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc
     2641 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg
     2701 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc
     2761 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc
     2821 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag
     2881 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac
     2941 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag
     3001 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat
     3061 cacgagcaat aaacccgccg gcaacgcccg cagcatcgcg accgtcatcg gtgagaagtt
     3121 cggcaggtat tgggtggtga caatgtaggt gctgccccaa atggcaggtg ctatcgctgt
     3181 gaacaataaa tcgggcgtgc gtaatgacat gcgattacaa gacctccgct acggcgatgg
     3241 gtttttgaga gttgcaggcg ccgcgatgac cgcaccctga cttggactgg ccctgcctcg
     3301 caccaatcca cctgcggcta tggccgcgat catgcgaggc ccgaaaatta cacgacctgt
     3361 acacgtccgc cgaacccgga acgcaacaga gcttcgtggg catcccgatc cgggtcgtag
     3421 cagcagtcct ggaccaaacg cacgtcgtag tccgcatcac tagcccaggc gacgcttgaa
     3481 agaacaacgc cggtggtgct tatcccggcc atgacaagcg tgcttacgcc gcgcgtccga
     3541 aggtcggcgt cgagcgccgt gccataaaag acgctggctc gcggacatgc atagaaaagg
     3601 tcgccccgtt cgatcgcaag cccttcgacg ggcagaccgg tgcgaaatcg tccgctcggg
     3661 agatacggtg agatttggcg attcgtcgcc ggcggcgcat gttcatactc ttcgccgagc
     3721 gaaaagttgg ggaacagcac gggccggccg cgcaaggcgt gctggcagcc gtgcagaccc
     3781 tgcgtgagat gaacgccgac aacctgcgca aggtgccggc cgatgcaccc acggccttca
     3841 tcaagccgcg ctggaagccg ctggtgatca ccccggaagg cctcgaccgg aaattctacg
     3901 aaatctgcgc cctgtccgag ctgaagaacg ccctgcgctc cggcgacatc tgggtcaagg
     3961 gctcgcggca gttccgcgac ttcgacgact acctgctgcc ggccgagaag ttcgccgcac
     4021 tcaagcgcga gcaggccctg cccctggcga tcaacccgaa cagcgaccag tacctggaag
     4081 agcgtttgca gctgctggac gagcagttgg ccaccgtcac ccgcctggcc aaggacaacg
     4141 agctgcccga tgccatcctc accgagtcag ggctgaaaat caccccgctg gatgcggcgg
     4201 tgccggatcg ggcgcaggcg ctgatcgacc aaaccagcca gttactgccg cgcatcaaga
     4261 tcaccgaact gctgatggac gtggacgact ggacgggctt cagccgccac ttcacccact
     4321 tgaaggacgg ggccgaggcc aaagacagga cgttgctgct gtccgcaatc ctcggtgatg
     4381 cgatcaacct cgggctgacc aagatggccg agtcgagccc cggcctgacc tacgccaagc
     4441 tgtcctggct gcaagcctgg cacatccgcg acgaaaccta ttcggcggcc ttggccgagc
     4501 tggtcaacca ccagtatcgc cacgcctttg ccgcccactg gggcgacggc acgacctcat
     4561 cctccgatgg ccagcgcttc cgcgcgggtg gccggggcga gagcaccggg cacgtcaacc
     4621 cgaagtacgg tagcgagccg ggacggctgt tctataccca tatctccgac cagtacgcgc
     4681 cgttcagcac ccgcgtggtg aatgtcggcg tccgcgattc cacctatgtg ctcgacggcc
     4741 tgctgtacca cgagtccgac ctgcggatcg aggagcacta caccgacacg gccggcttca
     4801 ccgatcacgt ctttgccctg atgcacctgc taggcttccg cttcgcgccg cgcatccgcg
     4861 acctcggcga aaccaagctg tacgtgccgc agggcgtgca agcctacccg acgttgcgcc
     4921 cgctgatcgg cggcaccctg aacatcaagc acgtgcgtgc ccactgggac gacatcctgc
     4981 gcctggccag ctcgatcaag cagggcaccg tcaccgcctc gctgatgctg cgcaagctcg
     5041 gcagctaccc gcgccagaac ggactggccg tggccctgcg cgagctgggc cggatcgagc
     5101 gcacgctgtt catcctggac tggctgcaaa gtgttgaact gcgccgccgc gtgcatgccg
     5161 gcctgaacaa aggtgaggcg cgcaactcgc tggccagggc ggtgttcttc aaccgccttg
     5221 gggaaatcag ggatcggagc ttcgagcagc agcgctaccg ggccagcggc ctcaacctgg
     5281 tgacggcggc tatcgtgctg tggaacacgg tgtacctgga acgcgccacc caggggttgg
     5341 t
//
LOCUS       DAZFYC010000553         3786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.guided.248, whole genome shotgun sequence.
ACCESSION   DAZFYC010000553 DAZFYC010000000
VERSION     DAZFYC010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.guided.248"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..316)
                     /locus_tag="ACHKW5_005878"
     CDS             complement(<1..316)
                     /locus_tag="ACHKW5_005878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7514967.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEGTRCTWNAPP
                     RGW"
     gene            complement(442..792)
                     /locus_tag="ACHKW5_005879"
                     /pseudo
     CDS             complement(442..792)
                     /locus_tag="ACHKW5_005879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(995..2008)
                     /gene="intI1"
                     /locus_tag="ACHKW5_005880"
     CDS             complement(995..2008)
                     /gene="intI1"
                     /locus_tag="ACHKW5_005880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO7514968.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2153..2650
                     /gene="dfrA12"
                     /locus_tag="ACHKW5_005881"
     CDS             2153..2650
                     /gene="dfrA12"
                     /locus_tag="ACHKW5_005881"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /protein_id="HFO7514969.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGK
                     VVVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVA
                     GGAEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVY
                     ARRNG"
     gene            2762..3052
                     /locus_tag="ACHKW5_005882"
     CDS             2762..3052
                     /locus_tag="ACHKW5_005882"
                     /inference="COORDINATES: protein motif:HMM:NF017990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="HFO7514970.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASA
                     SSYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
     gene            3058..>3786
                     /locus_tag="ACHKW5_005883"
     CDS             3058..>3786
                     /locus_tag="ACHKW5_005883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFO7514971.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQ
                     DLFEALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEAKQAALLQIVGGDKLII"
BASE COUNT          810 a         1089 c         1045 g          842 t
ORIGIN      
        1 accaacccct gggtggcgcg ttccaggtac accgtgttcc ttccttgacg aattcgatat
       61 gcacgccgcg ttgtgtcagc gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca
      121 ggcgatccat gctatgcacc accacggtgt cgccggtgcg ggcgaagctt atcagcgctt
      181 ccagttgcgg acgcttgaca tccttgccgg atgccttgtc gctaaaagcg cgatcaacct
      241 tgacgccttc cagttgccgt tccgggttct ggtcgaaggt gctgaccctg atatacccaa
      301 tgcgctgtcc agtcatggaa ttccctgcaa aatgtcaggg aagactctat gaccttcaac
      361 gagatatgtc aataaattca aaattcaatc ctatcctgac gcaatttaca catggcatct
      421 gacatcaggt tagggtatgc ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac
      481 gctggtttcg gtcgcagtgc tggcccgcgc ctggaagcgc tgatagcact ccagcccgca
      541 gaaatgctcc acgtactcgg ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt
      601 gcagcacacg cagcaactgg tgctcggttc attggcgttc atggtggtgt tcctccattg
      661 gttgacgaag ccgacgaagg ccgccgccgg catcggcctg gcgaacagga aaccctgcac
      721 cgtgtcgcaa cccgcctgcc gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc
      781 taccacctcc attgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
      841 tatagcagcg gaggggttgg atccatcagg caacgacggg ctgctgccgg ccatcagcgg
      901 acgcagggag gactttccgc aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg
      961 ggtagtgaat ccgccaggat tgacttgcgc tgccctacct ctcactagtg aggggcggca
     1021 gcgcatcaag cggtgagcgc actccggcac cgccaacttt cagcacatgc gtgtaaatca
     1081 tcgtcgtaga gacgtcggaa tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc
     1141 tgcggagcaa ggccgtcgcg aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga
     1201 tgcctgcttg ttctacggca cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat
     1261 ggcgacgcac gacaccgctc cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga
     1321 accacggcca ggaatgcccg gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa
     1381 cgccgctgcg gccctcggcc tggtccttca gccaccatgc ccgtgcacgc gacagctgct
     1441 cgcgcaggct gggtgccaag ctctcgggta acatcaaggc ccgatccttg gagcccttgc
     1501 cctcccgcac gatgatcgtg ccgtgatcga aatccagatc cttgacccgc agttgcaaac
     1561 cctcactgat ccgcatgccc gttccataca gaagctgggc gaacaaacga tgctcgcctt
     1621 ccagaaaacc gaggatgcga accacttcat ccggggtcag caccaccggc aagcgccgcg
     1681 acggccgagg tcttccgatc tcctgaagcc agggcagatc cgtgcacagc accttgccgt
     1741 agaagaacag caaggccgcc aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt
     1801 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca
     1861 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttggtaagc
     1921 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1981 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta
     2041 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2101 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttagcc atatgaactc
     2161 ggaatcagta cgcatttatc tcgttgctgc gatgggagcc aatcgggtta ttggcaatgg
     2221 tcctaatatc ccctggaaaa ttccgggtga gcagaagatt tttcgcagac tcactgaggg
     2281 aaaagtcgtt gtcatggggc gaaagacctt tgagtctatc ggcaagcctc taccgaaccg
     2341 tcacacattg gtaatctcac gccaagctaa ctaccgcgcc actggctgcg tagttgtttc
     2401 aacgctgtcg cacgctatcg ctttggcatc cgaactcggc aatgaactct acgtcgcggg
     2461 cggagctgag atatacactc tggcactacc tcacgcccac ggcgtgtttc tatctgaggt
     2521 acatcaaacc ttcgagggtg acgccttctt cccaatgctc aacgaaacag aattcgagct
     2581 tgtctcaacc gaaaccattc aagctgtaat tccgtacacc cactccgttt atgcgcgtcg
     2641 aaacggctaa ccattccgtc aacgggacgc caaaatgctg cgcattttgg ttccctccgc
     2701 tgcgctccgg ctctcgttac gtccaacgtt agcaccactg aaacccagct ttatttagct
     2761 catgtttatt caaacggcat ttagcttttc aggcgttatt cagtgcctgt tttgcctttt
     2821 ttccgggctt cgcctgcatg ggctgcgcag gttttcagtc tttttggcct ctagcccttg
     2881 cgtagcaagc gcaagcagct atcgtttttg cagtgctgtg ccgcctcggt ggcgcagcgt
     2941 tttttcacgg ttagcgcccg tcgccaaatt caagttatcc gttttggctt ctggttctaa
     3001 catttcggtc aagccgaccc gcattctgcg gtcggcttac ctcgcccgtt agacatcatg
     3061 agggaagcgg tgaccatcga aatttcgaac caactatcag aggtgctaag cgtcattgag
     3121 cgccatctgg aatcaacgtt gctggccgtg catttgtacg gctccgcagt ggatggcggc
     3181 ctgaagccat acagcgatat tgatttgttg gttactgtgg ccgtaaagct tgatgaaacg
     3241 acgcggcgag cattgctcaa tgatcttatg gaggcttcgg ctttccctgg cgagagcgag
     3301 acgctccgcg ctatagaagt cacccttgtc gtgcatgacg acatcatccc gtggcgttat
     3361 ccggctaagc gcgagctgca atttggagaa tggcagcgca atgacattct tgcgggtatc
     3421 ttcgagccag ccatgatcga cattgatcta gctatcctgc ttacaaaagc aagagaacat
     3481 agcgttgcct tggtaggtcc ggcagcggag gaattctttg acccggttcc tgaacaggat
     3541 ctattcgagg cgctgaggga aaccttgaag ctatggaact cgcagcccga ctgggccggc
     3601 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcaat aaccggcaaa
     3661 atcgcgccga aggatgtcgc tgccgactgg gcaataaaac gcctacctgc ccagtatcag
     3721 cccgtcttac ttgaagctaa gcaagcggca ctgttgcaaa tagtcggtgg tgataaactt
     3781 atcatc
//
LOCUS       DAZFYC010000554         1628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2304S59192
            SAMN43283704-rid23073063.guided.330, whole genome shotgun sequence.
ACCESSION   DAZFYC010000554 DAZFYC010000000
VERSION     DAZFYC010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283704
            Sequence Read Archive: SRR30325234
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351496.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351496.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 04:48:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,885
            CDSs (total)                      :: 5,792
            Genes (coding)                    :: 5,510
            CDSs (with protein)               :: 5,510
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 119 of 282
            Pseudo Genes (incomplete)         :: 156 of 282
            Pseudo Genes (internal stop)      :: 62 of 282
            Pseudo Genes (multiple problems)  :: 50 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283704-rid23073063.guided.330"
                     /serotype="O123:H 2"
                     /isolate="2304S59192"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHKW5_005884"
     CDS             259..1206
                     /gene="stxA1a"
                     /locus_tag="ACHKW5_005884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO7514972.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAP
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHKW5_005885"
     CDS             1216..1485
                     /gene="stxB1a"
                     /locus_tag="ACHKW5_005885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO7514973.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          436 a          277 c          394 g          521 t
ORIGIN      
        1 ttgccagcct cccccagtgg ctggcttttt tatgtccgta acatcctgtg tatcaataaa
       61 tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg accagatatg ttaaggttgc
      121 agctctcttt gaatataatt atcattttca ttacgttatt gttacgttta tccggtgcgc
      181 cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa tatagttatc gtatggtgct
      241 caaggagtat tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt
      301 atcttttcag ttaatgtggt tgcgaaggaa tttaccttag acttctcgac tgcaaagacg
      361 tatgtagatt cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca
      421 tcaggaggta cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt
      481 gatgtcagag ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa
      541 cgaaataatt tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt
      601 gctgattttt cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt
      661 agctatacca cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc
      721 cattcgttga ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag
      781 tctgtggcaa gagcgatgtt acggtttgtt actgtgacag ctgaagcttt acgttttcgg
      841 caaatacaga ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg
      901 actgctgaag atgttgatct tacattgaac tggggaaggt tgagtagtgt cctgcctgat
      961 tatcatggac aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt
     1021 ctgggaagcg tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg
     1081 gcacctgatg agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac
     1141 aataaaatat tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc
     1201 agttgagggg gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca
     1261 gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat
     1321 gatgacgata cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat
     1381 cttcagtctc ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat
     1441 gcctgtcata atggaggggg attcagcgaa gttatttttc gttgacttag aatagctcag
     1501 tgaaaatagc aggcggagat tcataaatgt taaatacatc tcaattcagt cagttgttgc
     1561 cggtctgata atagatgtgt tagaaaattt ctgcatggtg aatccccctg tgcggagggg
     1621 cgactggt
//
